LOCUS       DASWPO010000001         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2142738, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000001 DASWPO010000000
VERSION     DASWPO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142738"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VGC69_00005"
     CDS             <1..1140
                     /locus_tag="VGC69_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0293696.1"
                     /translation="TNEGIQVYPREITDFTEPLPDDDYWQRVAEHIRTRFGPATQISW
                     GVNQVPGFWVSFQIDQDLYWLVFEREQIGLTGGIEWLGWGATALLLSLVGAAISVGFV
                     NRPLSRLARAAQVLSRGETPMPLPEQGPLEIRNLNSSFNRMAKDLRQAETDRELMLAG
                     ISHDLRTPLARMRLEIEMSEVSDDTRQAIDNDLSQIDHSIGQLMEYARPAGTLPQMAT
                     DISTVLADLFERERTHTASLGGELNARIEPGLRARVTALDLKRIVGNLIENARRYGRS
                     ADGQAHLVMTVQSEGNTLAIEVSDRGPGIAPEEVERLLRPFSRGEAARTGVSGAGLGL
                     AIVERLLKHVGGSLRMLPRSGGGLTARIELPKAKFRNYQLDAEDQ"
     gene            1247..1795
                     /locus_tag="VGC69_00010"
     CDS             1247..1795
                     /locus_tag="VGC69_00010"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0293697.1"
                     /translation="MKTVGDKLEPFKVAGVKPGFNHHEENGVSAFEEITESSFPDKWK
                     IIYFYPKDFTFVCPTEIVGFDKLAKDFADRDAVLMGGSVDNEFVKLAWRREHPDLDKL
                     GHYQFGDTNGSLIDQLGVREKEAGVALRATFIVDPDNTIQHVSVNNLNVGRNPEEILR
                     LLDGLQTDELCPCNRMVGGATL"
     gene            1851..>1920
                     /locus_tag="VGC69_00015"
     CDS             1851..>1920
                     /locus_tag="VGC69_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0293698.1"
                     /translation="MEFLTAIKDQLPDWAKDIRLNLD"
BASE COUNT          380 a          684 c          557 g          299 t
ORIGIN      
        1 accaacgaag gcatccaggt ctatccgcgc gagatcaccg acttcaccga gcccctgccc
       61 gacgacgatt actggcagcg cgtggccgag cacatccgca cgcgcttcgg ccccgccacg
      121 cagatctcct ggggcgtcaa ccaggtgccc ggcttctggg tgagcttcca gatcgaccag
      181 gacctgtact ggctggtgtt cgagcgagag cagataggcc tgacgggcgg catcgagtgg
      241 ctgggctggg gcgccaccgc cctgctcctc tccctggtgg gcgcggccat cagcgtgggc
      301 ttcgtcaacc ggccgctgtc gcggctggcg cgcgccgccc aggtgctgtc gcgcggcgaa
      361 acgcccatgc ccctgcccga acagggtccg ctcgagatcc gcaacctcaa ttcgtccttc
      421 aaccgcatgg ccaaggacct gcgccaggcc gagaccgacc gcgagctgat gctggccggc
      481 atctcgcacg acctgcgcac gccgctggcg cgcatgcgcc tggaaatcga gatgagcgaa
      541 gtctccgacg acacgcgcca ggccatcgac aacgacctca gccagatcga ccacagtatc
      601 ggccagctca tggaatacgc ccgccccgcg ggcacgctgc cgcagatggc caccgacatc
      661 tccacggtgc tggccgacct cttcgagcgc gagcgcaccc atacggcctc gctgggcggc
      721 gagctcaacg cccgcatcga acccggcctg cgcgcccgcg tcacggcgct cgacctcaag
      781 cgcattgtcg gcaacctgat cgagaacgcc cgccgctacg gccgctccgc cgacggccag
      841 gcccacctgg tcatgaccgt gcaatccgag ggcaatacgc tggcaatcga agtctccgac
      901 cgcggccccg gcatcgcccc cgaagaagtc gaacgcctgc ttcgcccctt ctcccgcggc
      961 gaagccgccc gcaccggcgt cagcggcgcc ggcctggggc tggccatcgt cgagcgcctg
     1021 ctcaagcacg tgggcggttc gctgcggatg ctgccgcgca gcggcggcgg cctgacggcc
     1081 cggatagagc tgcccaaggc caaatttaga aactaccaat tagacgccga agatcaatag
     1141 cgtagaatta ctttcacccc aggttgcgca gcggcaatcg cgaagaattc ttaggcagca
     1201 cctgtctgcc tattcgttca ctccaacaac ctatcaggga gcatccatga aaaccgttgg
     1261 cgataagctc gaaccgttca aggtcgcagg cgtcaagccc ggcttcaacc atcacgaaga
     1321 aaacggcgtg tccgctttcg aagagatcac cgaaagctcc ttccccgaca agtggaagat
     1381 catctacttc tacccgaagg acttcacctt cgtgtgcccg accgaaatcg tcggcttcga
     1441 caagctggcc aaggacttcg ccgatcgcga cgccgtgctg atgggcggct cggtcgacaa
     1501 tgaattcgtc aagctggcat ggcgccgcga acatcccgac ctcgacaagc tgggccacta
     1561 ccagttcggc gacaccaacg gctcgctgat cgaccagttg ggcgtgcgtg aaaaggaagc
     1621 cggcgttgcc ctgcgcgcca ccttcatcgt cgaccccgac aacaccatcc agcacgtgtc
     1681 ggtgaacaac ctgaacgtcg gccgcaaccc cgaagaaatc ctgcgcctgc tcgacggcct
     1741 gcaaaccgac gaactgtgcc cctgcaaccg catggtcggc ggcgcgacgc tgtaagcctc
     1801 atggcccggc cccgccgggt tttttcagcc ttttaaaata ggcaaaagct atggaattcc
     1861 tcaccgcaat caaggaccag ctgccggatt gggccaagga catccgcctg aacctggacg
//
LOCUS       DASWPO010000002         6318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2678482, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000002 DASWPO010000000
VERSION     DASWPO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2678482"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1097..1303
                     /locus_tag="VGC69_00020"
                     /pseudo
     CDS             1097..1303
                     /locus_tag="VGC69_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            1313..2095
                     /gene="hpaH"
                     /locus_tag="VGC69_00025"
     CDS             1313..2095
                     /gene="hpaH"
                     /locus_tag="VGC69_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEY0293699.1"
                     /translation="MLDDQDRARAAELLMAAERAGEPTTQLAQVFPGMEIADSYAIQK
                     RIMDCKIAQGARLRGHKIGLTSKAMQSTVGIDEPDYGHLLDTMFFQDGETVSTARMIV
                     PRVEVELAFVLGKALHGPGVTLFDVLDATDYVVPALEIIDGRSKYPRTIVDNIADNAA
                     CAGIILGGRPVRPMDIDLRWVGALLLKNGVIEESGVSAAVLGHPALGIAWLANKLAQH
                     ETRLQAGHVVLAGSFTRTVAVGSGDTLHADYGKLGSISVHFS"
     gene            2105..2878
                     /locus_tag="VGC69_00030"
     CDS             2105..2878
                     /locus_tag="VGC69_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEY0293700.1"
                     /translation="MDLPVNRFKRALRGQRHQTGLWVTLGQANSTELVAASGFDWLLL
                     DTEHTPATLPTVMAQLQAAAAYPCHPVVRPSWNDKVQIKQYLDIGAQTLLLPYVQNAE
                     QAREAVSGIRYAPRGMRGVGGTTRATRYGRVADYMRSCEAELCLLVQAETGEALENLD
                     AMLAVDGVDGVFIGPADLAASLGYPGEPGHAKVVAEVEAAIARVRAAGKAPGVLTTDE
                     ALARRYMAAGSLYTAVGVDAAILARHCDQLAQRFRQEPD"
     gene            complement(2886..3617)
                     /locus_tag="VGC69_00035"
     CDS             complement(2886..3617)
                     /locus_tag="VGC69_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY0293701.1"
                     /translation="MTDSILHPADDTLATRLAALRARMAAACARAGRPPEGVALLPVS
                     KTFGVDLIRAAAGLGLTRFGENKTQEIRQKTGPLADLNLQWVLIGHLQTNKAKEAARD
                     AAEVQSLDRLELAEALQRRLDLEGRAIDVLVQVKTSPEDSKSGLPPAELPAFLRTVAR
                     DYPALRVKGLMTLAVNSDDPQAVRACFRQLRELRDDLRGAGIANVALDRLSMGMSGDF
                     ELAIEEGSTEVRIGSALFGARRYPA"
     gene            3735..4973
                     /locus_tag="VGC69_00040"
     CDS             3735..4973
                     /locus_tag="VGC69_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0293702.1"
                     /translation="MSTAAPNYVFAAPFQEIYVPPIRSLVPYASRPGTVSLAGGYPAP
                     ELFDVEGLQEASDRVMPNLRGLLQYANVEGQASLLAALIKLSGERGIACKTDEIMITG
                     GAQHGIALLARVLLAPGDNAILEDPAFPNTVQALRYTGANVHAVPSGTDGVDLDALDE
                     MAARLKPKLVSVVASFSNPGGATISRERRLKLLQLAVKHRFLLIEDDPYGELRYSGEK
                     VPPIMALADAESRPWLAYTSTMSKTMSPGMRVGWLIAPAEIRRRCVSAKAADDMANPA
                     WIQEIAAQYLTGGRYHEHVPHIRDAYGARCAALGDALAGILGDRISFVRPEGGMFFWV
                     RLAGDIDATRLLPYAIEKEVVYVPGKGFYLDPSRHADLFAMRLSFVTVNEERLKLGVQ
                     RLAQAIEACEANEPVSIALA"
     gene            complement(5026..5997)
                     /locus_tag="VGC69_00045"
     CDS             complement(5026..5997)
                     /locus_tag="VGC69_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0293703.1"
                     /translation="MNLKKWLLAAPLALACAWANADTYPSHTITMVVPFNAGGPTDTV
                     ARVLAQALSKQLGASVVVENIGGAGGTLGAEHIAKTTPDGYTLLLHHIGLATAQTLYR
                     KLPYDTRTAFSPIGLVTEAPMALMVRPGLPVHSLPDLVALMKQKPGALTFGHSGLGGA
                     DQLCGLLLQTAVGSSLQLVPYRGNAPVMTDMLGGRIDMTCGQLTNATGYIKSGQVRGL
                     AVTAAARSPVLPDLPITREAGLPQLDITVWHGLFAPAGTPAPVIDRLAAALRGAMADP
                     QLSQRFLDIGTTPVSAALASPAALKQRLDSEIDRWAPIIQAAGQYAD"
     gene            complement(6054..>6318)
                     /locus_tag="VGC69_00050"
     CDS             complement(6054..>6318)
                     /locus_tag="VGC69_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0293704.1"
                     /translation="ALAQRHPTWFKLSAPYRLGGLPAASLLPDIVASIGPRRLLWGSD
                     WPCTRHEACRNFADLCRLPALPAQPGLSLMQCCAANAAALYMT"
BASE COUNT         1202 a         1964 c         1959 g         1193 t
ORIGIN      
        1 atcaacttct atggggaaaa aatcaagtaa cttttatgaa gaaaatagtg aaagaaaaag
       61 actaaaagag catttttgca gcctagagtt taatatgttc cggcatggtt tgtgataaat
      121 aatccctaaa tccaatagac aaaaaatgat tgcaattata ttgacagaat aacctatact
      181 aaagacaaac atattgaccc cataataaat catatacaat tcagatccct aattcattac
      241 caataaacac taaatttgca ataacatggc aaactacaga gcaatttagg acttaagagt
      301 ctgttcgcca tcagcttttt ggtttagcta tttagcttat ttgcctatat acttataaaa
      361 aaaaagctta tttgcctata tacagctttt tcaagcctca ctttttctag gtataacaaa
      421 tatactttaa tgaactttca gcaaataaac aataagcaat tagaaaaaaa ctgcatccaa
      481 acacacactt agtctctatt tgggataact tattttggac aacttatttt taaaagtgag
      541 gcccgtttaa ataagtgcag ttttaataag ctttacctag aaaaaagtga ggcccgtttt
      601 atccaacttc aatcctatta tctttttctt tcctttaatc ttatttgttg ctgaaatttc
      661 ttcttaaacc cttcttctag attagaccta agttctcctc atcacaccac tttgtttacc
      721 ccattgctat caagtaagat tgggaagtga tctgatagag gtcgttgtag taagcgctga
      781 cccaaattag aaaaaatgca cttcccaatt aagtgaaacc aaaaatctat ctaacatgga
      841 cattgaaggt gaatcaagat tattcgacca agtaaaagaa ccgcccctcc aaaggaaaat
      901 ccaacaaagc atgttctgat ataaactcag aaaaatccca cctcctttca ctttcctttt
      961 tttttttata ggtaaagata tagatttttt caaaggagaa tgttgtagtg tgcataaatc
     1021 atgcacacta gagcatcatg agtaagagat aaagaagaaa taaactctat aacatgaacc
     1081 gcagttctag aaaaagttcc cattcgccga attgatccgc tacatctcga tctggaccac
     1141 gctggaaccc ggcgacgtca tttccaccgg cacgccggtg ggcgcgggcg tgcgctttac
     1201 gccgccgcgc tttctcaagc ccggcgacgt ggtggaagtg gaggtgccca gcatcggcac
     1261 gctgtccaac cctgtggccg atgatcgggc cacgaccctc tgaggatatt ccatgctcga
     1321 cgaccaggac cgtgcgcgcg ccgctgaatt gctgatggcc gccgagcgcg ccggcgaacc
     1381 gaccacgcag cttgcccaag tatttccagg catggagatt gccgattcct atgcgatcca
     1441 gaagcgcatc atggactgca agatcgccca gggcgcccgc ctgcgcggcc acaagatcgg
     1501 cctgacgtcc aaggccatgc agagcaccgt gggcatcgac gagccggact acgggcatct
     1561 gctcgacacc atgtttttcc aggacggcga gaccgtctcc accgcaagga tgatcgtgcc
     1621 gcgcgtggag gtcgaactgg ccttcgtgct gggcaaggcg ctccacggcc ccggcgtcac
     1681 gctgttcgac gtgctggacg ccacggacta cgtcgtgccg gcgctggaga tcatcgatgg
     1741 ccgcagcaaa tatccgcgca ccatcgtcga caacatcgcc gacaatgccg cctgcgccgg
     1801 catcatcctg ggcggccgtc ccgtgaggcc gatggacatc gacctgcgct gggtcggggc
     1861 cctcctgctc aagaacggcg tgatcgagga gtcgggcgtg tcggcggcgg tgctggggca
     1921 cccggcgctg ggcatcgcct ggctggccaa caagctggcg cagcatgaga cccgcctgca
     1981 ggcgggccat gtcgtcttgg ccggctcgtt cacccggacg gtggcggtgg ggtctggtga
     2041 cacgctgcat gcggactacg gcaagctggg cagcatctcc gtccattttt cttaaggaac
     2101 tttcatggat cttcccgtca atcgtttcaa gcgcgcgctg cgcggccaac gtcaccagac
     2161 cggcctgtgg gtgaccttgg gacaagccaa tagcaccgaa ctggtggcgg ccagcggctt
     2221 cgactggctg ttgctggaca cggaacatac gcccgcgacg ctgcccacgg tcatggcgca
     2281 gctgcaggcg gccgcggcct atccctgtca tccggtggtg cggccgtcct ggaacgacaa
     2341 agtgcagatc aagcagtacc tggacatcgg cgcgcagact ttgctgctgc cttatgtgca
     2401 gaacgcggag caagcgcgcg aggccgtgtc gggcatacgc tacgcgccgc gcggcatgcg
     2461 cggtgtcggc ggcaccacgc gcgcgacccg gtatggccgc gtggccgatt acatgcgcag
     2521 ctgcgaagcg gagctgtgcc tgctggtaca ggccgagacc ggagaagcgc tggaaaacct
     2581 ggatgccatg ctcgcggtcg atggcgtgga cggcgtcttt atcggcccgg ccgatctcgc
     2641 cgccagcctg ggctatcccg gagagcctgg gcatgccaag gtcgtggccg aagtggaagc
     2701 cgcgatcgcg cgcgtgcggg ccgccggcaa ggcgcccggc gtgctgacga cggacgaggc
     2761 cctggcgcgc cgctatatgg cggcgggcag cctgtacacg gcggtgggcg tggacgccgc
     2821 cattcttgcg cggcattgcg accagttggc gcagcgcttt cggcaggaac cggactgacg
     2881 catcgtcatg ccgggtagcg ccgcgcgccg aatagcgcgc tgccgatgcg tacttcggtc
     2941 gagccttctt cgatggcgag ttcgaaatcg ccgctcatgc ccatcgacag tcggtccagc
     3001 gctacgttcg cgatgcctgc gccgcgcagg tcgtcgcgca gttcgcgcag ctggcgaaag
     3061 cacgcgcgca cggcctgcgg gtcgtccgag ttgacggcca gggtcatcag gcccttgacg
     3121 cgcagggccg ggtagtcgcg ggccacggtg cgcaggaagg ccggcagttc agcgggcggc
     3181 aggccagatt tgctgtcttc cggcgaggtc ttcacctgca ccagcacgtc gatggcgcgg
     3241 ccttcgaggt cgagccggcg ttgcagggcc tcggcgagtt ccaggcggtc gagcgactgc
     3301 acttcggcgg cgtcgcgcgc ggcttccttg gccttgttgg tctgcaggtg gccgatcagc
     3361 acccattgca ggttcaggtc ggccagcggg ccggttttct ggcggatttc ctgggtcttg
     3421 ttttcgccga agcgggtcag gcccagcccg gccgccgcgc ggatgaggtc gacgccgaag
     3481 gtcttgctca cgggcaacag ggccacgccc tcgggggggc ggccggcccg cgcgcaggcg
     3541 gcggccatgc gggcgcggag ggccgccagg cgggtggcga gggtgtcgtc ggcaggatgg
     3601 aggatggaat cagtcatggc aggcgtgtcg ccgcgggcgg gaatggcccc attatcctac
     3661 gcgccgtgtc gcgttgaccg ggtagggggc ggggcataga atcgtccgat tgtcatatcc
     3721 tctgccgtca tcacatgtcc accgcagcac cgaattacgt ttttgccgcc ccgtttcagg
     3781 aaatctacgt cccccccatc cgctcgctcg tgccctacgc gtcacggccg ggcacggtgt
     3841 ccctggcggg cggctacccg gcgcccgagc tgttcgacgt cgaaggcctg caggaggctt
     3901 ccgaccgcgt catgcccaat ctgcgcggcc tgctgcagta cgccaacgtc gagggccagg
     3961 ccagcttgct ggcggcgctc atcaagctca gcggcgaacg cggcatcgcc tgcaagaccg
     4021 acgagatcat gatcaccggc ggcgcgcagc acggcatcgc gctgctggcg cgcgtgctgc
     4081 tcgcgcccgg cgacaacgcc atactcgaag acccggcctt ccccaacacc gtccaggccc
     4141 tgcgctatac cggcgccaac gtgcacgccg tgccctccgg caccgacggc gtggacctcg
     4201 acgcgctcga cgaaatggcc gcgcgcctca agcccaagct cgtgtccgtg gtggcctcgt
     4261 tctccaaccc gggcggcgcc accatcagcc gcgagcggcg gctgaagctg ctgcagctgg
     4321 cggtcaagca ccgcttcctg ctgatcgagg acgatcccta cggcgagctg cgctattccg
     4381 gcgaaaaggt tccgcccatc atggccctgg ccgatgccga gtcgcgtccc tggctggcct
     4441 atacctcgac catgtccaag accatgtcgc ccggcatgcg cgtgggctgg ctgatcgcgc
     4501 cggccgagat ccgccgccgc tgcgtcagcg ccaaggccgc cgacgacatg gccaaccccg
     4561 cctggatcca ggaaatcgcc gcgcagtacc tgaccggcgg ccgctaccac gagcacgtgc
     4621 cgcacatccg cgacgcctac ggcgcgcgct gcgccgcgct gggcgacgcg ctcgccggca
     4681 tcctgggcga ccggatcagc ttcgtcaggc ccgaaggcgg catgttcttc tgggtgcgcc
     4741 tggccggcga tatcgacgcc acccgcctct tgccctacgc catcgaaaaa gaagtggtct
     4801 acgtgccggg caagggcttc tacctcgacc cgtccaggca cgccgacctg ttcgccatgc
     4861 gcctgtcctt cgtcacggtc aacgaagaac ggctcaagct gggcgtgcag cgcctggccc
     4921 aggccatcga ggcctgcgaa gccaacgagc cggtgtcgat cgcgctggcc tgatggccgt
     4981 cgcgctacgt tgcttgcatc gccgcctgat ccggcccgcc gcgcctcaat ccgcgtattg
     5041 ccctgctgcc tggatgatcg gcgcccatcg gtcgatttcg ctgtcgagcc gctgcttcag
     5101 cgcggccggg ctcgccagcg cggcgctgac cggcgtcgtg ccgatatcga ggaagcgctg
     5161 cgacaattgc ggatcggcca tcgcgccgcg cagcgcggcc gccaggcgat cgatgacggg
     5221 cgcgggcgtg ccggccggcg cgaacagccc gtgccagacg gtgatatcga gctggggcag
     5281 cccggcttca cgggtgatcg gcagatcggg caataccggc gagcgggccg cggcggtgac
     5341 cgccaggccg cgcacctggc cggatttgat atagccggtc gcgttggtca attgcccgca
     5401 ggtcatgtcg atgcgcccgc cgagcatatc ggtcatcacc ggcgcattgc cgcggtaggg
     5461 cacgagctgc aggctgctgc cgacggcggt ttgcagcagc aggccgcaga gttgatcggc
     5521 gcctcccagg ccggaatggc cgaaggtcaa ggcgccgggc ttctgcttca tcagcgcgac
     5581 caggtcgggc aggctgtgca cgggcaggcc ggggcgcacc atcagcgcca tcggcgcttc
     5641 ggtgaccagg ccgatcgggc tgaacgcggt gcgcgtgtcg tacggcagct tgcgatacag
     5701 cgtctgcgcg gtggccagtc cgatgtgatg cagcagcagc gtatagccgt cgggggtggt
     5761 cttcgcgatg tgctcggcgc cgagcgtgcc gccggcgccg ccgatgtttt ccaccacgac
     5821 gctggcgccg agctgcttgc tcagcgcttg cgccagcacg cgcgcgacgg tatcggtcgg
     5881 gccgccggca ttgaaaggaa ccaccatcgt gatggtatgc gacggatagg tatcggcgtt
     5941 cgcccaggcg caagcgagcg ccagcggcgc ggcgagcagc catttcttca gattcattgt
     6001 cagtctccgg ttttttttag gttgcgcggc cactcgtcga ttggccgtcg ttgttatgtc
     6061 atgtacaggg cggcggcatt ggccgcgcag cattgcatca gggacaggcc gggttgcgcc
     6121 ggcagcgcgg gcaggcggca caggtcggcg aaattccggc aggcttcgtg ccgcgtgcaa
     6181 ggccagtcgc tgccccacag caggcgccgc ggcccgatgc tagcgacgat gtcgggcagc
     6241 aggctggccg ccggcaggcc gcccagccga tacggcgccg acagcttgaa ccaggtcgga
     6301 tgccgctgcg ccagcgcg
//
LOCUS       DASWPO010000003         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1607798, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000003 DASWPO010000000
VERSION     DASWPO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607798"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VGC69_00055"
     CDS             <1..823
                     /locus_tag="VGC69_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEY0293705.1"
                     /translation="QQREQLDLFRALPGDGMAPRDSQDLMAFPFFSLAKSRRTAPIDF
                     RSGNITIRVEGTAKHGIATIWDADVLIWAASQIVEARDAGIRPSRWIHATPYEILRFI
                     GRGTSLNDYQRLKAALDRLQSTSVATSIRETTGRRLHRFSWVNEWRELADASGTPLGI
                     ELILPDWFYAGVLDAALVLTIDPAYFRLKGGIERWLYRLVRKHGGRQEHGWQFDFRHL
                     HRKSGSAARFSDFAYDLRVLVARQSLPGYVLGIEPMPGERLELLTFRPVPSTARG"
     gene            1089..>1673
                     /gene="parA"
                     /locus_tag="VGC69_00060"
     CDS             1089..>1673
                     /gene="parA"
                     /locus_tag="VGC69_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HEY0293706.1"
                     /translation="MIVALLNQKGGVGKTTLATHIAGELAMRGLHVILLDADPQGSSL
                     DWTQRRSQQGLPRLFSAVGLARETLHQEAPELARRADHVIIDGPPRIAALARSALLAA
                     ERVLIPVQPSPYDVWASAEMVALIREAQVFRPALRAAFVINRRVSTTIIGREARQSLA
                     EQPLPALRAEVRQRIVFADSVAAGRLARETAPDSA"
BASE COUNT          281 a          568 c          546 g          278 t
ORIGIN      
        1 gcagcagcgg gaacagctcg acctgttccg cgcgctgccg ggcgacggca tggcgccgcg
       61 cgacagccag gacttgatgg cctttccgtt cttctcgctg gcgaagtcgc ggcgcacggc
      121 gccgatcgac ttccgcagcg ggaacatcac catccgcgtg gaaggcacgg ccaagcatgg
      181 catcgccacg atatgggacg ccgacgtttt gatttgggcg gcctcgcaga tcgtggaagc
      241 gcgcgacgca ggcattcgtc cgtcgcgctg gatacacgcc acgccatacg aaatcctgcg
      301 cttcatcggg cgcggcacgt ccctcaacga ctaccagcgc ctgaaagccg cgctcgaccg
      361 actgcaatcg accagcgtgg cgacttccat ccgcgaaacc accgggcggc gcctgcaccg
      421 cttttcgtgg gtgaacgaat ggcgcgagct ggccgacgcc agcggcacgc cgctgggcat
      481 cgagctgatc ctgcccgact ggttctatgc cggcgtgctc gatgccgctc tggtgctgac
      541 catcgacccg gcctatttcc gcttaaaggg cggcatcgag cgctggctgt accgcctggt
      601 gcgcaagcac ggcgggcggc aggaacacgg ctggcaattc gacttccggc atctgcaccg
      661 caagtccggc agcgcggcgc gcttctcgga cttcgcctac gacctgcgcg tgctggtcgc
      721 gcgtcagtcg ctgcccggct acgtcctggg catcgagccg atgccgggcg aaaggctgga
      781 actgctgacc ttccggcccg tgccgtctac ggcacgggga taactgcggg aaaaggtgtg
      841 gacggcctcg tgctatcagg agtaccgggt atcgtgctat cgggagtacg cctatcgtgc
      901 tatcaggagt acgaaaacgt cgcaaatcca gtatcggcgc gggttttcgg cccctctaac
      961 tacctaactt caaaatctct aacttttagt agaagcgcgc cgtttcggtg gacaaccacg
     1021 aaacggcacg gcgaacagcg ttttgcatgg caggcaaagc ccggctttcc aacatgggag
     1081 gccgcgtcat gatcgttgcc ttgctcaacc agaaaggcgg cgtcggcaag accacgctcg
     1141 ccacccacat cgcgggcgag ctggcgatgc gcggcctgca cgtcatcctg ctggatgccg
     1201 acccgcaagg ttcatcgctg gactggacgc agcggcgtag ccagcagggc ttgccacggc
     1261 tgttcagcgc cgtgggcctc gctcgcgaaa cgctgcatca ggaagcgccg gagctggccc
     1321 gccgcgccga tcacgtcatc atcgacggcc cgccgcgcat cgccgccttg gcgcgctccg
     1381 cgctgctggc ggccgagcgc gtgctgatcc cggtgcagcc cagtccctat gacgtgtggg
     1441 ccagcgccga gatggtggcg ctgatccgcg aggcgcaggt gttccggcct gcgctgcgcg
     1501 cggccttcgt catcaaccgg cgcgtcagca ccaccatcat cggcagggag gcgcggcaat
     1561 cgctggccga acagccattg cctgcgctgc gcgccgaggt tcggcaaagg atcgtctttg
     1621 ccgacagcgt ggccgctggc cggctcgccc gcgagacggc gcccgacagc gcc
//
LOCUS       DASWPO010000004        14798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_268956, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000004 DASWPO010000000
VERSION     DASWPO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14798
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268956"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VGC69_00065"
     CDS             complement(<1..262)
                     /locus_tag="VGC69_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0293707.1"
                     /translation="MKRLPFFDNPVLRGQLPMWRARLVLILLFLGFATLAGRAMFLQG
                     LSTDFLQQQGERRYERTLTLSASRGKILDRNGVVLASSVPARA"
     gene            complement(259..549)
                     /gene="ftsL"
                     /locus_tag="VGC69_00070"
     CDS             complement(259..549)
                     /gene="ftsL"
                     /locus_tag="VGC69_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY0293708.1"
                     /translation="MARLSFIMAAVLMASAVSLVTSRYQARQLFIELGRGDAQAHDLD
                     MDWRRLQLDRAELTRNARVDRMARDDLKMIPIVPDRTIYLNQAPVSQAGGAQ"
     gene            complement(549..1670)
                     /gene="rsmH"
                     /locus_tag="VGC69_00075"
     CDS             complement(549..1670)
                     /gene="rsmH"
                     /locus_tag="VGC69_00075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY0293709.1"
                     /translation="MEFEHRPVLLEPTVDALVLAGFGGKGASRSASRDDGAGAARLQR
                     GVYVDGTFGRGGHSRELLRRLGREARLVVFDKDPQAIAVAQGLAADDGRVSVVHGGFS
                     SVGEELAAMGITHIDGLMLDLGVSSPQIDDAERGFSFMREGPLDMRMDTSRGVTAEQW
                     LAQASIDDMREVIADYGEERFAFQVAKAIAARRAAGPLRTTLELAECVASAVRTREKG
                     QHPATRTFQALRIYLNRELEELASTLAAALELLAPGGRLAVISFHSLEDRMVKQCIAA
                     AARPGAAQARLPLRESEMPQPLFRSLGRVLASEEEIAVNPRARSAVLRAAERTTTPLA
                     SGAASFVPGLPGADRARKGSRAPGRAKRAAPARSAKGAS"
     gene            complement(1679..2107)
                     /gene="mraZ"
                     /locus_tag="VGC69_00080"
     CDS             complement(1679..2107)
                     /gene="mraZ"
                     /locus_tag="VGC69_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY0293710.1"
                     /translation="MFQGSSALTLDTKGRISIPTRYRDALAEQAGGSLTLTRHPDGCL
                     LVYPRPEWEKKREQIAGFPMTARALQRLLLGNAQDVELDGSGRVLIAPELRTAAGMTR
                     EVMLLGMGAHFELWDAASLARREAEDLAQGMPEVLNQFSF"
     gene            complement(2433..2735)
                     /locus_tag="VGC69_00085"
     CDS             complement(2433..2735)
                     /locus_tag="VGC69_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293711.1"
                     /translation="MPEPLRLLIHAPTAQALQRARRNAANLLQAEPDAQVEIVANAAA
                     VAAALAEPHPTDSHLLLCRNTLAAIGAEAPPTLRVVPAAVLHLAQRQRDGWAYLRA"
     gene            complement(2728..3471)
                     /locus_tag="VGC69_00090"
     CDS             complement(2728..3471)
                     /locus_tag="VGC69_00090"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0293712.1"
                     /translation="MAAVMRFDDFSYIHPGADAPVLRGVSLDIEAGSFVAVVGGSGVG
                     KSTLLRAAAGLAEPGSGGGVHFHAGTAPGRRPRAVVFQDSRLMPWRRVGANVAYGLDG
                     LGLPAEEKQRRVRDALALTGMSGYEARWPHQLSGGQAQRVGIARALAVKPDLLLMDEP
                     FSAVDAITRRHLQAELIRVWQASGAAVLFVTHDIEEAVFLADRVVVLGGRPAGVAGVY
                     DIGLPRPRDRSAADFARHVAGVAQLLEHA"
     gene            complement(3459..4202)
                     /locus_tag="VGC69_00095"
     CDS             complement(3459..4202)
                     /locus_tag="VGC69_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0293713.1"
                     /translation="MRRALYGVLGVAIFVALWKAAQVWEWAPRGTLPDPFALPQALLS
                     EWREGRLLPAVASSLVHYVWGLSLGTVLGCVVGLAAATIERFDMLQGYLARILRPIPP
                     LAWVVFAIAWFKVSHAGAAFVIAIGVFWVNYFATYAAVRNVDPRFYELARAFGHGGYA
                     ARAWTVTLPAIAPGALAGMRTGIGQAWMTLIAAELLGVPGMGQEMNAAAGVGAYDAVV
                     VYMLAISLVYTVCDLAYGRLEKRVLQWRP"
     gene            complement(4274..5293)
                     /locus_tag="VGC69_00100"
     CDS             complement(4274..5293)
                     /locus_tag="VGC69_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0293714.1"
                     /translation="MIRKLYTAVLLVAGLSGAAPAALAAGPALEIGYLPILSDAQLFV
                     ALEDGMLHGGGLDSPKLVSFQSGPAITQALIAGQLDVAYVGIGPALVARAKGADIKVV
                     AANNKGQVSMLALGSLAPYFGSGDPSTAFARFAKDKGRKAVIASYPKGAVPETALQYW
                     LRNVIKADLADIEFVYQGEAQIQQSLMTGAIDGAVVLEPTTTSVQRRVPDARVVANGT
                     QLFPNQPGAVLLVREKLIAEHPEVVRALVQAQIDATKVLRDDPAKAAGYVRKYVGGGR
                     LPADLVEQSIRNSHDQFEPDPRTIVQATIKLQDFQAGLGTQAERVDVGQLFDSRFFES
                     VGRAQ"
     gene            5423..6733
                     /locus_tag="VGC69_00105"
     CDS             5423..6733
                     /locus_tag="VGC69_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0293715.1"
                     /translation="MVIYLPLRAHRPSRPIARALWAALLSLAAAFPAARAQPPGPGRQ
                     PAWQAAWSTSLQAIPDLPNPPPLYKKPDVAGRTVRQIIVPALDGRDARLRVSNRYGTQ
                     PLVIERAVIARSDGGAGLVAGSAQAVTFGGRPALTLAPGAEADSDGISFSVVRDKPLA
                     VSLTLGPRQTLQAWHRVANRINFISSEGDHAADVPGTAFHGRITQFAWLTQLSVSGAR
                     AGGLAAIGDSITDGMRSTLGTRHSWPEDLARRAAAEGLPLAVHNAGISGNRLLSDSPC
                     YGERVVGRFEHDAATLPGVRAVILLAGINDINFAAMPARRGLDCDFPHTQVGANDLIN
                     GYRRVIEAAHQHGLRIFGATLTPAALPPGNEAIRAEVNHWIRAGGGFDGVVDFDAALR
                     DPQHPTRLLPAYNSGDDIHPSDAGYAAMAQAVPLTLLRDISSHP"
     gene            6801..7811
                     /locus_tag="VGC69_00110"
     CDS             6801..7811
                     /locus_tag="VGC69_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY0293716.1"
                     /translation="MRLISSRAARYALASLLLGTLPYHAAAQAAENRVIILDSAEASL
                     TLIDQDTRKVLGTMPTGKEPHHLMITPDHNSLIVANSVSNSLMFVDPHSGQLQGTLEG
                     IDDPYQLGFSPDRKWFATAALRLDRVDIYHYDGKNFTVAKRIPLSKTPSHLTFSSDSR
                     FVFVTLQDSGELAAIDLATQTLAWRMPVGKAPAGVWMTPGDKYLLVGMTGEDDVAVVD
                     WRQHQVVKRIPTGRAAHNFRSLDDGQHVAVSNRVSNTISIIDYNTLTKTADITGLLPG
                     PDDMELSADHRYLWVTFRFARHVGVIDLTTRTLVDTIKVGRSPHGIYFANHAPVLSPN
                     AN"
     gene            7827..8825
                     /locus_tag="VGC69_00115"
     CDS             7827..8825
                     /locus_tag="VGC69_00115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0293717.1"
                     /translation="MNSLLAMIDNAISAMQTWLFVDLIQPAMFQLGLMGYDEDTYDAL
                     YWVLVGVLQVVASYALLRPLEAWRPVEAWRDRAQTRPDILYTWIAKLGLINTAFFFLL
                     QPLFNHLQSLVSVHGVPNIDLDRLWPGVTDQAIVSFLIYLVVLDFAGYWYHRWQHGIS
                     IWWELHAVHHSQQQMSLWSDDRNHFLDDILQAAFFAALALFIGVAPIQYVALVAVGNF
                     LQSVQHANARLPFGPVLERVLVSPAFHRWHHALDIPLGRTGGWNFGILFPWWDMMFGT
                     ASWGHAAEPTGIREQLPAPQGAGRSYGKGLLAQQWLALGRIAGHLRPRRAQRQASP"
     gene            complement(8864..9258)
                     /gene="rnpB"
                     /locus_tag="VGC69_00120"
     ncRNA           complement(8864..9258)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGC69_00120"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9344..11161
                     /locus_tag="VGC69_00125"
     CDS             9344..11161
                     /locus_tag="VGC69_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0293718.1"
                     /translation="MAAATLITLTNVQLAYGHHPLLDHADLAIQQGERIGLIGRNGAG
                     KSSLLKLLDGRTQPDDGDISRASGLRVATVEQEPELLETATIYDVVCEPDAHDEDWSR
                     PARVQATLERLGLPGELVVSGLSGGMRKRVALARALVDEPDLLLLDEPTNHLDFDGIA
                     WLETLLRNWKGAAIIITHDRRFLDAVTSRIVELDRGKLLSFPGNFSQWQERKAQWLES
                     ERLEQARFDKLLAQEEVWIRKGIEARRTRNEGRVRRLEQLRVDRAERRERIGNVNLAL
                     ASGQRSGKLVAELKHVSKAYGERQVVRDYSTTILRGDRIGLVGPNGAGKTTLLRIILG
                     LLEPDEGTVRLGTNVSVAYFDQMREQLDEEAPLADVISPGSEWVEIGGTRKHIMSYLG
                     DFLFSPARAGSPVKSLSGGERARLLLARLFARPANVLVLDEPTNDLDIETLELLESLL
                     QEYSGTVLLVSHDRAFLNNVVTQTIASDGDGEWRDYVGGYDEWIAQRPARENNDAKTG
                     GAGPAKAEPKAPERSKPARAVRLSSWEERELSELPDAIAALEARQTELSGKLTDGSLY
                     HKAPEEADGINAELAKLESELEEKFARWETLEAKRAAAT"
     gene            complement(11311..12369)
                     /gene="pyrC"
                     /locus_tag="VGC69_00130"
     CDS             complement(11311..12369)
                     /gene="pyrC"
                     /locus_tag="VGC69_00130"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0293719.1"
                     /translation="MPAASTLTLIRPDDWHLHLRDGAALEAVVGDSARQFARAIIMPN
                     LRPPVTTTAQALDYRSRIVRALDKAGVAPGVFTPLMTLYLTDNTPADEIVRARESGLV
                     HAVKLYPAGATTNSDAGVTDLLGKCGPVLQALEKCGMPLLVHGEVTDPEIDVFDREAV
                     FIDRVMYPLRRAYPALKVVFEHITTKDGAHYVRDAEGPVAATITPQHLLYNRNAIFQG
                     GVRPHWYCLPILKRETHRQALLEAATSGSPRFFLGTDSAPHARGLKEHACGCAGCYTA
                     LHAMELYATAFDSVGKLDKLEAFASFNGPAFYGLPRNAGTLTLQRGEYSVPEEIPFGN
                     TTLVPLAAGEALEWRVVV"
     gene            complement(12436..13629)
                     /locus_tag="VGC69_00135"
     CDS             complement(12436..13629)
                     /locus_tag="VGC69_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY0293720.1"
                     /translation="MYARSALESIRLFHDELTALRRDLHAHPELGFEEVRTSGIVAGA
                     LEALGIEVHRGIGKTGVVGVIRGKRCDSGRMIGLRADMDALPMTEDNTFGHKSGKSGL
                     MHGCGHDGHTAVLIGAARYLAQTRNFDGTAVLIFQPAEEGRGGAQAMLDDGLFEMFPC
                     DAIYAMHNWPGLKPGMIGVNPGPMMAAADRFEILIEGRGGHGAHPYQTIDPVTVAGHM
                     ITALQSIVSRNVNPLDSAVVSIGAMQAGNPGAMSVIPGEARLVGTVRTFRKTVQELIE
                     SRMHQLAGSIAGAFGAKATVKYERLFPATINTPQHANLVADIATEMIGKENVVRDLVP
                     SMGSEDFSFMLQQKPGAYFRLGQGGAEEGCVLHNPRFDFNDAVIPMGSAMFCALAEHG
                     MPLAD"
     gene            13780..14058
                     /locus_tag="VGC69_00140"
     CDS             13780..14058
                     /locus_tag="VGC69_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY0293721.1"
                     /translation="MATTSKTTTARKPNAAFMKPLTPSPELAAVIGAQAVPRTEVTKK
                     IWDYIKQHNLQDAANKRNINADAKLLPIFGKDQVTMFELTKLVNAHLK"
     gene            complement(14193..14558)
                     /locus_tag="VGC69_00145"
     CDS             complement(14193..14558)
                     /locus_tag="VGC69_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293722.1"
                     /translation="MSDQTTSVPTAGPDLRLLTHVAYGLFALGFLTGGILGVATLAAV
                     VLLYVKRSDTAGTVYAGHFDWLLRTFWWSVLWLFLSGILTMIFIGWLGVLITLVWALY
                     RLIKGWLALFEGAAPTSYT"
     gene            complement(14666..14778)
                     /gene="rrf"
                     /locus_tag="VGC69_00150"
     rRNA            complement(14666..14778)
                     /gene="rrf"
                     /locus_tag="VGC69_00150"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2628 a         5287 c         4597 g         2286 t
ORIGIN      
        1 tggcgcgcgc cggcacgctg gaggccagca ccacgccgtt gcggtcgagg atcttgccgc
       61 gcgaggccga cagggtcagc gtgcgctcgt agcgccgctc gccctgctgc tgcaggaagt
      121 ccgtggacag gccctgcagg aacatcgcgc ggccagccag cgtcgcgaat cccagaaaca
      181 gcaggatcag caccagccgc gcgcgccaca tgggcaactg gccgcgcagc acggggttgt
      241 cgaagaacgg cagccgcttc attgcgcgcc tcccgcctgg ctgacgggcg cctggttcag
      301 gtagatcgtg cgatcgggca cgatggggat catcttgagg tcatcgcgcg ccatgcggtc
      361 cacgcgcgca ttgcgggtca gctccgcgcg gtcgagctgc aggcgccgcc aatccatgtc
      421 gaggtcatgc gcctgcgcat cgccgcggcc cagctcgatg aagagctgcc gcgcctgata
      481 gcggctggtc accagcgaca cggccgacgc catcagcacc gcggccatga tgaagctcag
      541 gcgagccatt acgaagcccc cttggcgctg cgcgcagggg cagcccgctt ggcgcggccc
      601 ggcgcccgcg agcctttcct ggccctgtcg gcgcccggca gtccgggcac gaacgaggcc
      661 gcgccgctcg cgagcggggt cgtggtgcgc tcggccgcgc gcagcaccgc cgagcgggcg
      721 cgcgggttca cggcgatttc ttcttcgctg gccagcacgc gccccaggga acgaaagagc
      781 ggctgcggca tttcgctctc gcgcaggggc agcctggcct gggccgcgcc cggcctggcc
      841 gccgcggcga tgcactgctt gaccatgcgg tcttcgagcg aatgaaagct gatcaccgcc
      901 aatctccccc ctggcgccag cagctctaga gctgccgcga gggtgctcgc gagctcttcg
      961 agctcccgat tgaggtaaat ccgtaaagcc tgaaaggtgc gtgtggccgg atgctgcccc
     1021 ttttcgcgag tgcggacggc actggcgacg cactcggcaa gctcgagcgt ggtgcgcagc
     1081 ggccctgctg cgcggcgagc agcaatcgcc tttgcaacct gaaaagcaaa ccgttcttcg
     1141 ccataatccg ctatgacctc ccgcatgtca tcgatactgg cttgcgccag ccattgctcc
     1201 gccgtaaccc cgcggctggt atccatccgc atgtccagcg ggccttcccg catgaacgag
     1261 aaaccacgct ctgcatcgtc gatctgaggc gaggacacgc ccagatccaa catcaatccg
     1321 tcgatgtgcg taatgcccat cgccgccagc tcttcaccca cggaggaaaa gcctccgtgc
     1381 accacgctca cccggccgtc atcggccgcc aggccctgcg ccaccgcgat ggcctgggga
     1441 tccttgtcga atacgaccag gcgggcttcc cgccccaggc ggcgcagcag ttcgcggctg
     1501 tggccgccgc ggccgaacgt gccgtccaca tagacccccc gctgcaggcg cgccgcgccg
     1561 gcgccatcgt cccgactcgc cgaccgcgaa gcgcccttgc cgccgaaacc ggccagcacc
     1621 aaagcgtcca ctgtcggctc gagcagcacg ggccgatgtt cgaattccat aaggggcttc
     1681 agaacgaaaa ctggttcaac acttccggca tgccctgtgc cagatcttcc gcctcgcggc
     1741 gggccagaga ggcggcatcc caaagctcga agtgggcgcc cataccgagc agcatcactt
     1801 cgcgcgtcat accggcagcg gtgcgcaatt caggggcgat cagcacgcgg ccggagccgt
     1861 cgagctcgac atcctgggcg ttgcccagca gcaggcgctg cagggcgcgg gccgtcatgg
     1921 ggaagccggc gatctgctcg cgcttctttt cccactcggg ccggggatag accagcaggc
     1981 agccgtcggg gtggcgggtg agcgtcaggc tgccccccgc ctgctcggcg agggcgtcac
     2041 gataccgggt cgggatcgag atccgcccct tggtatccaa cgtgagtgcg ctacttccct
     2101 gaaacaccct taaccccgcc cttggttccg ccgcctgatt gaacgtcgct acgccaatct
     2161 attccactta tttgcacaaa atcctacttt ttcccactct acgggaacgg tcttggccgg
     2221 tcaagccaca agttgcattt ttttcaatta caacaaggac ttagactgat tcttgcgggc
     2281 aatgaacagg ggagaaaaag aaaaaaccct cttgaaaatc agagggttgt agaggtaact
     2341 caaagtcttt cctaaaaaat attgcgccct cggccgaggg cgtccacgcg ccagagatgt
     2401 aaacaaaagc caagaaaggg cggaacgcgc cctcaggctc gcaggtaggc ccagccgtct
     2461 cgctggcgtt gcgccaggtg cagcacggcc gccggcacca cccgcagggt gggcggggcc
     2521 tcggcgccga tagccgccag ggtattgcgg cacagcagca ggtgggagtc ggtcgggtgc
     2581 ggttcggcca gggcggcagc gaccgccgcg gcattggcga cgatctcgac ctgggcgtcg
     2641 ggctcggcct gcagcaggtt ggcggcattg cggcgggcgc gctgcagggc ttgcgccgtg
     2701 ggcgcgtgga tcaagaggcg caggggttca ggcatgttca agcagttggg cgaccccggc
     2761 gacatgccgg gcgaaatccg ccgccgaccg atcgcgcggc cggggcaggc cgatatcgta
     2821 cacgcccgcc acgccggccg gacggccgcc cagcacgacc acgcggtcgg ccaggaaaac
     2881 ggcctcctcg atgtcgtgcg tcacgaacag cactgccgcg cccgacgcct gccagacccg
     2941 gatcagctcg gcctgcaggt gccggcgcgt gatggcgtcg accgcgctga agggttcgtc
     3001 catcagcagc aggtcgggct tgacggccag ggcgcgggcg atgcccacgc gctgcgcctg
     3061 cccgccggag agctggtgcg gccagcgcgc ctcgtacccg gacatgccgg tcagcgccag
     3121 cgcgtcgcgc acgcggcgct gcttctcctc ggccggcagg cccagcccgt ccagcccata
     3181 ggccacattg gcgcccacgc gccgccacgg catgaggcgg ctgtcctgga acaccaccgc
     3241 gcgcggccgc cgccccggcg cggtcccggc atggaagtgc acgccgccgc cgctgcccgg
     3301 ttcggccagc cccgccgcgg cgcgcagcaa ggtggacttg cccacgcccg agccgcccac
     3361 gacggcgacg aagctgcccg cctcgatgtc cagcgacacc ccgcgcagca ccggcgcgtc
     3421 cgcgcccggg tggatatagg agaaatcgtc gaaacgcatc acggccgcca ttgcagcacc
     3481 cgcttttcca ggcgtccgta ggcaagatcg cacaccgtgt agaccagcga gatcgccagc
     3541 atgtacacca ccaccgcgtc ataggcgccc acgccggcgg ccgcgttcat ttcctgcccc
     3601 atgccgggca cgcccagcag ctcggcggcg atcagggtca tccaggcctg gccgatgccg
     3661 gtgcgcatgc cggccagcgc gcccggcgcg atggccggca gcgtcaccgt ccaggcgcgc
     3721 gcggcatagc cgccatggcc gaacgcccgc gccagctcgt aaaagcgcgg atccacattg
     3781 cgcacggcgg cgtaggtggc gaagtaattg acccagaaca caccgatggc gatcacgaat
     3841 gccgcgcccg catggctgac cttgaaccag gcaatggcga acaccaccca ggccagcggc
     3901 gggatgggcc gcaggatgcg cgccagatag ccttgcagca tatcgaagcg ctcgatggtg
     3961 gcggccgcca ggccgacgac gcaacccagc accgtgccca gcgacagccc ccagacgtaa
     4021 tgcaccaggc tggaagccac cgcgggcagg agccgccctt cccgccactc gctcagcagg
     4081 gcctgcggca gcgcgaacgg gtcgggcagg gtgccgcgcg gcgcccactc ccacacctgc
     4141 gcggccttcc atagcgccac gaagatcgcg acgcccagca cgccgtacag cgcgcggcgc
     4201 atgcccgcca tgccggggcg ccgcgcccgg gcggcggcgc gcggcgcctc gccggcgatg
     4261 acggcctggc tgctcattgc gctctgccca cggattcgaa gaagcgggaa tcgaacaact
     4321 gacccacgtc gacgcgctcg gcctgcgtgc ccagcccggc ctggaagtcc tgcagcttga
     4381 tcgtggcctg cacgatggtg cgcggatcgg gctcgaactg gtcatgcgaa ttgcggatgg
     4441 actgctcgac caggtcggcc ggcaggcggc cgccgcccac gtatttgcgc acatagccgg
     4501 ccgccttggc cgggtcgtcg cgcagcacct tggtggcgtc gatctgggcc tgcaccagcg
     4561 cgcgcaccac ctcgggatgc tcggcgatca gcttctcgcg caccagcagc acggcgcccg
     4621 gctggttcgg gaaaagctgc gtgccgttgg ccaccacgcg cgcgtccggc acgcggcgct
     4681 gcacgctggt ggtggtgggc tcgagcacca ccgcgccgtc gatggcgccg gtcatgagcg
     4741 actgctggat ctgcgcctcg ccctggtaga cgaactcgat atcggccagg tcggccttga
     4801 tcacgttgcg cagccagtac tgcagcgccg tctcgggcac cgcgcccttc ggatagctgg
     4861 cgatcacggc cttgcggccc ttgtccttgg cgaagcgggc gaaggcggtg gacgggtcgc
     4921 ccgatccgaa atacggcgcc agcgatccga gcgccagcat gctcacctgc cctttgttgt
     4981 tagcggccac caccttgatg tcggcgccct tggcccgcgc caccagggcc gggccgatgc
     5041 cgacataggc cacgtcgagc tggcccgcga tcagcgcctg cgtgatcgcc gggccgctct
     5101 ggaaagacac cagcttgggc gagtccagcc cgccgccgtg cagcatgccg tcctcgaggg
     5161 cgacgaacaa ttgcgcatcg gaaagaatgg gcaggtagcc gatctccagg gcggggccgg
     5221 ccgcgagtgc ggcgggggcc gcgccggaga ggcccgccac gagcaggacg gcggtgtaca
     5281 acttgcggat catcgtttct ttttcctgta gggtctgcgc tgcgcccagg ccagtcagct
     5341 atttggaggg tctctctggc aaactacggc gcagctattt actaataatt ttaagaaact
     5401 taatttagat tctttattaa ttatggttat atacttacct ctccgggcac ataggccatc
     5461 gcgccctatc gcccgtgcgc tgtgggccgc cctgttgtcc ctggcggcgg ccttccccgc
     5521 cgcccgagcc cagcctcccg ggccgggccg gcagcccgcc tggcaggcgg cctggtccac
     5581 atcgctgcaa gccatccccg atctgcccaa tccgccgccg ctctataaaa agcccgacgt
     5641 ggccgggcgc accgtccggc agatcatcgt gcccgccctg gacgggcgcg acgcgcgcct
     5701 gagagtcagc aaccgctacg gcacccagcc gctggtcatc gaacgggccg tcatcgcccg
     5761 ttcggacggc ggcgccggcc tggtcgccgg ctcggcccag gccgtgacat tcggcggccg
     5821 ccccgccttg accctggcgc ccggcgccga ggccgacagc gatggcatct ccttcagcgt
     5881 cgtccgcgac aagccgctgg ccgtcagcct gaccctgggt ccgcgccaga ccctgcaggc
     5941 ctggcatcgc gtcgccaacc ggatcaattt catctcgagc gaaggcgacc atgccgcgga
     6001 cgtgccgggc acggcctttc acggccgcat cacccagttt gcctggctca cgcagctgtc
     6061 cgtatccggc gccagggccg gcgggctggc cgccatcggc gactccatca ccgacggcat
     6121 gcgttcgacg ctgggcacgc ggcacagctg gcccgaggac ctggccaggc gcgccgccgc
     6181 cgagggcctg cccctggccg tccacaatgc cggcatcagc ggcaatcgcc tgctgtccga
     6241 ctcgccctgc tatggcgaac gcgtcgtcgg ccgcttcgaa cacgatgccg ccaccctgcc
     6301 cggcgtgcgc gccgtcatcc tgctggccgg catcaacgac atcaacttcg ccgccatgcc
     6361 cgcccggcgc ggcctcgact gcgacttccc ccatacccag gtcggcgcga acgatttgat
     6421 caacggctat cgccgggtaa tcgaggccgc gcaccagcac ggcctgcgca tcttcggcgc
     6481 aacgctcacg cccgccgcgc tgccgccggg caacgaggcc atccgtgccg aggtcaatca
     6541 ctggatccgc gcgggcggcg ggttcgacgg cgtcgtcgac ttcgacgcgg cgctgcgcga
     6601 cccgcagcat cccaccagat tgctgccggc ctacaacagc ggcgacgaca tccatcccag
     6661 cgatgccggc tatgccgcca tggcgcaggc cgtgccgctg acacttttac gcgacatttc
     6721 gtcgcacccg taaaccggcg cgataccggc gaacggcata aaatctcgtc tgcttcactc
     6781 tttggtcatc tgcgcccatc atgcgcctca tttcatcccg ggctgccagg tatgccctgg
     6841 ccagcctgct gctgggaacc ctgccgtacc acgcggccgc ccaggcagcc gaaaaccgcg
     6901 tcatcatcct cgattcggct gaagcctccc tcaccctcat cgaccaggac acccgcaagg
     6961 tgctgggcac catgccgaca ggcaaggagc cgcaccacct catgatcacg ccggaccata
     7021 attccctgat cgtggccaac tcggtgtcca acagcctgat gttcgtcgac ccgcacagcg
     7081 ggcagctgca aggcacgctg gaaggcatcg acgaccccta tcaactgggc ttctcgcccg
     7141 accgcaaatg gttcgccacc gcggccctgc gcctggaccg ggtggatatc taccactacg
     7201 acggcaagaa cttcaccgtc gccaagcgca tccccctgag caagaccccc agccacctga
     7261 cgttttcctc cgactcgcgc ttcgtcttcg tcaccttgca ggactccggc gaactggccg
     7321 ccatcgacct ggccacccag acgctggcgt ggcgcatgcc ggtcggcaag gccccggcgg
     7381 gcgtctggat gacgccgggc gacaaatacc tgctggtcgg catgaccggc gaagacgacg
     7441 tggccgtggt cgactggcgc cagcaccagg tcgtcaagcg catcccgacc ggacgcgccg
     7501 cccacaactt ccgctcgctg gacgacggcc agcacgtcgc ggtcagcaac cgggtgtcca
     7561 acaccatcag catcatcgac tacaacacgc tgaccaagac cgccgacatc accggcctgc
     7621 tgcccgggcc ggacgacatg gaactttcgg ccgaccaccg ctatctatgg gttacgttcc
     7681 gcttcgcccg ccacgtgggc gtgatcgacc tgaccacgcg caccctggtc gacaccatca
     7741 aggtgggacg ttcgccccat ggcatctatt tcgccaacca cgccccggtc ctgagcccga
     7801 acgccaactg atcgccccgt ccgcccatga acagcctgct cgccatgatc gacaacgcca
     7861 tctctgccat gcagacctgg ctgttcgtcg acctcatcca gccggccatg ttccagctcg
     7921 ggctgatggg ctatgacgaa gacacctacg acgccctcta ctgggtcctc gtcggcgtgc
     7981 tccaggtcgt ggcgagctat gccctgctgc gtcccctgga agcctggcga cccgtggaag
     8041 cctggcgcga ccgcgcccag actcggcccg acatcctcta cacctggatc gccaagctgg
     8101 gcctgatcaa cacggccttt ttcttcctgc tgcagcccct gttcaaccac ctgcaaagcc
     8161 tggtctcggt ccatggcgtg cccaatatcg acctggaccg gctgtggccc ggcgtgaccg
     8221 accaagctat cgtctccttc ctgatctacc tggtcgtgct ggatttcgcc ggatactggt
     8281 accaccgctg gcagcacggc atcagcatat ggtgggaact gcacgcggta caccacagcc
     8341 agcagcagat gtcgctgtgg tccgacgacc gcaatcactt cctcgacgac atcctgcagg
     8401 cggccttctt cgccgcgctg gccctgttca tcggcgtggc gcccatccaa tatgtggcgc
     8461 tggtggccgt cggcaacttc ctgcaaagcg tgcagcacgc caatgcccgc ctgccgttcg
     8521 gcccggtgct cgaacgcgtg ctggtcagcc ccgcgttcca tcgctggcac cacgccctgg
     8581 acattcccct gggcaggacg ggcggatgga acttcggcat cctgttcccc tggtgggaca
     8641 tgatgttcgg caccgcctcc tggggccatg ccgccgaacc taccggcatc cgcgagcagc
     8701 tgcccgcccc gcagggcgca ggccgctcgt acggcaaggg cctgctggcc cagcaatggc
     8761 tggccctggg ccgcatcgcc ggccacctgc ggccgcgccg cgcgcagcgg caagcctcgc
     8821 cttaaggccc gggcgggcac gccgccaccg cgcccgaatt cagaagcaaa acagatctat
     8881 aggccggatt ctgtacgccg ggtcgccccg gcgggcaatc attcctctgc gccagccatt
     8941 gctggctggc tctagccatc tacccgcatg ctcgagcgag ccgcccttcc cggccgaaac
     9001 cgtgcgcatg cctatttgat gttgctccgg gtagaggtta ccgcgtttca ccctgcagcc
     9061 ccgccgcccg ttgccggacg gcgcggcacg gagatggccg tgccggtgcg cgacgtgcgc
     9121 gcccatgctg cagactcgtc tctgtggccc tattcctcgc ccggcaagcc ggacggacgg
     9181 ccgttagccg ctaccctgcc ctgtggagtc cggaccttcc tcgatgggtt gccccgccgc
     9241 gattgcccga tctgttctgc gggggcattt tagtacaccc ggccggccac agccccgcgg
     9301 cggttcctgc gacaatagcg gtctgctcga ttcttcccac gatatggctg ccgcgactct
     9361 catcaccctc accaacgtcc agctggccta cggccaccac cccctgctgg accatgccga
     9421 cctcgccatc cagcaaggcg agcgcatcgg cctgatcggc cgcaacggcg cgggcaagtc
     9481 ctcgctgctg aagctgctcg acggccgcac gcaacccgac gacggcgaca tctcgcgcgc
     9541 ctcgggcctg cgcgtggcca cggtcgaaca agagcccgag ctcctcgaga ccgcgacgat
     9601 ctacgacgtg gtctgcgaac ccgacgccca tgacgaagac tggtcccgcc cggcgcgtgt
     9661 gcaggccacc ctcgaacgcc tgggcctgcc cggcgaactc gtcgtctcgg gcctgtccgg
     9721 cggcatgcgc aagcgcgtgg ccctggcccg cgccctggtc gacgaacccg acctgctcct
     9781 gctcgacgaa cccaccaacc acctggactt cgacggcatc gcctggctcg agaccctgct
     9841 gcgcaactgg aagggcgcgg ccatcatcat cacgcacgac cggcgctttc tcgatgccgt
     9901 cacctcgcgc atcgtcgaac tcgaccgcgg caaactgctg agcttccccg gcaatttctc
     9961 gcaatggcag gagcgcaagg cgcagtggct ggaatccgaa cgcctcgagc aggcccgctt
    10021 cgacaagctg ctggcccagg aagaagtctg gatacgcaag ggcatcgaag cgcgccgcac
    10081 ccgcaacgaa gggcgggtgc gacggctgga acaactgcgc gtcgatcggg ccgaacgccg
    10141 cgaacgcatc ggcaacgtca acctggccct ggcctcgggc cagcgctcgg gcaagctcgt
    10201 ggccgaactc aagcacgtga gcaaggccta cggcgagcgc caagtggtgc gcgactactc
    10261 caccaccatc ctgcgcggcg accgcatcgg cctggtcggc cccaacggcg ccggcaagac
    10321 caccctgctg cgcatcatcc tgggcctgct cgaacccgac gaaggcacgg tccgcctggg
    10381 caccaatgtc tcggtcgcgt atttcgacca gatgcgtgaa cagctcgatg aagaggcccc
    10441 cctggccgac gtcatcagcc ccggcagcga atgggtggaa atcggcggca cccgcaagca
    10501 catcatgagc tatctgggcg acttcctgtt ctcgcccgcg cgcgccggct cgccggtcaa
    10561 gagcctctcg ggcggcgaac gcgcccgcct gctgctggcc cgcctgttcg cccgcccggc
    10621 caacgtgctg gtgctcgacg aacccaccaa cgacctcgac atcgaaaccc tggaactgct
    10681 cgaatccctg ctgcaggaat acagcggcac cgtgctgctc gtcagccacg accgcgcctt
    10741 cctcaacaat gtggtcacgc agaccatcgc cagcgacggc gacggcgagt ggcgcgacta
    10801 tgtcggcggc tacgacgaat ggatcgcgca gcgccccgcg cgcgaaaaca acgacgccaa
    10861 gaccggcggc gccggtccgg ccaaggccga acccaaggcg ccagaacgca gcaaacccgc
    10921 ccgcgccgtc cgcctgagct cctgggaaga acgtgaattg agcgaactgc ccgacgccat
    10981 cgcggcgctc gaagccaggc agaccgaact atcgggcaaa ctgaccgacg gcagcctcta
    11041 ccacaaggct cctgaagaag ccgacggcat caacgccgaa ctcgccaagc tggaaagcga
    11101 gctggaagaa aaattcgccc gctgggaaac gctggaagcc aagcgcgcag cagcaaccta
    11161 agacgtgaaa cgccaggctt gagacgtccg gctcgaaacg ccaggtttga gaccccttga
    11221 gccgaggacc ccggaggaat ccgaagcgca gggggcgtct tagcccccga gcatcggaat
    11281 tccgcagggc agcctcggcg gctcaagaac tcaaacaacc acccgccact ctagcgcctc
    11341 gcccgccgcc aacggcacca gcgtcgtatt gccaaaaggt atttcctccg gcaccgaata
    11401 ctcgccgcgc tgcaacgtca gcgtgcccgc attgcgcggc aggccgtaga aagccggccc
    11461 gttgaagctg gcaaaagctt ccagcttatc cagcttgccc acgctgtcga acgccgtggc
    11521 atacagctcc atggcatgca gcgccgtgta gcagcccgcg caaccgcagg catgctcctt
    11581 caggccgcgc gcgtgcggcg cgctgtcggt gcccaggaaa aaccgcgggc tgccgctggt
    11641 ggcggcctcc agcagggcct ggcgatgcgt ctcgcgcttg agaatgggca ggcaatacca
    11701 gtgcggccgg acgccgccct ggaaaatcgc gttgcggttg tagagcagat gctgcggcgt
    11761 gatcgtcgcc gccaccggcc cttcggcatc gcgcacataa tgcgcgccgt ccttggtcgt
    11821 gatgtgctcg aacaccacct tcagggccgg ataagcgcgg cgcaggggat acatgacgcg
    11881 gtcgatgaac accgcctcgc ggtcgaacac gtcgatctcg ggatcggtca cttcgccgtg
    11941 caccagcagg ggcatgccgc acttctccaa ggcctgcagc accggcccgc acttgcccag
    12001 cagatcggtc acgcccgcat ccgaattggt ggtggcgcct gccggataca gcttcacggc
    12061 atgcaccagg ccggactcgc gcgcgcgcac gatttcatcg gccggcgtat tgtccgtgag
    12121 atagagcgtc atcagcggcg taaacacgcc cggcgcgacg ccggccttgt ccagcgcccg
    12181 cacgatgcgg ctgcgatagt ccagcgcctg cgccgtcgtg gtcaccggcg gcctcagatt
    12241 gggcatgatg atggcccgtg caaactggcg ggcgctatcg ccaaccaccg cctccagcgc
    12301 cgcgccatcg cgcaggtgca ggtgccaatc gtcggggcgg atcagggtca gggtcgaagc
    12361 agcgggcatg ggattcgagg atatcgtcgt ggaaagtggc ccggccgtgg gggtggacag
    12421 cccaggccgg caggatcaat cggccagcgg catgccatgc tcggccaggg cgcagaacat
    12481 cgcgctgccc atcggaatca cggcatcgtt gaagtcgaag cgaggattgt gcagaacgca
    12541 gccctcttcg gcgccgccct gccccaggcg gaaatacgcg ccgggcttct gctgcagcat
    12601 gaaggaaaag tcttccgagc ccatggacgg caccaggtcg cgcaccacgt tttccttgcc
    12661 gatcatctcg gtcgcgatat cggccaccag gttggcgtgt tgcggcgtat tgatcgtggc
    12721 cgggaacagg cgctcgtatt tcaccgtggc cttggcgccg aaagcgccgg cgatcgaccc
    12781 cgccagctgg tgcatgcgcg actcgatcag ctcctgcacc gtcttgcgga aagtgcgcac
    12841 cgtgcccacc agcctcgcct cgccggggat cacgctcatc gcgcccgggt tgcccgcctg
    12901 catggccccg atcgacacca ccgcggaatc cagcggattc acattgcgcg acacgatgct
    12961 ctgcaaagcc gtgatcatgt gccccgccac cgtcaccgga tcgatcgtct ggtacggatg
    13021 ggcgccgtgg ccgccgcgcc cttcgatcag gatctcgaag cgatcggccg ccgccatcat
    13081 ggggcccgga ttcacgccga tcatgcccgg cttcaggccc ggccagttat gcatcgcata
    13141 gatggcatcg caggggaaca tctcgaacag gccgtcgtcg agcatggcct gcgcgccgcc
    13201 ccggccctct tccgccggtt gaaaaatcag cacggccgtg ccgtcgaagt tgcgcgtctg
    13261 ggccagatag cgtgccgcgc cgatcagcac ggccgtatgc ccatcgtggc cgcagccgtg
    13321 catcaacccc gacttgcccg acttgtggcc gaaggtattg tcttccgtca tgggcagcgc
    13381 atccatgtcg gcacgcaggc cgatcatgcg gccgctgtcg cagcgcttgc cgcgtatcac
    13441 gccgaccacg cccgtcttgc cgatgccgcg atgcacttcg atgcccagcg cctccagcgc
    13501 acctgccaca atgcccgatg tacgcacttc ttcgaagccg agttcggggt gggcatgcag
    13561 atcgcgccga agcgcagtga gttcgtcatg gaacaggcgg atggattcga gagctgagcg
    13621 ggcgtacata gttgaaatgc aaattacggc aagtgagagg gaagagaccc tttattttag
    13681 ttgctacaga ggataaatgg ctttgtcttt aagggtgaga cacgttatgc tgcggcccgc
    13741 aggacaaatt ttctaactac cagaaaggag agccggatca tggccactac ctccaagacc
    13801 acgaccgcgc gcaagcccaa tgccgcgttc atgaagccgt tgaccccgag ccccgaactg
    13861 gctgccgtca tcggcgcaca ggcagtgccc cgcaccgaag tcaccaagaa aatttgggac
    13921 tacatcaagc aacacaacct gcaagatgcc gccaacaagc gcaacatcaa tgccgacgcc
    13981 aagctgctcc ccatcttcgg caaagaccag gtcaccatgt tcgaactgac caaactggtc
    14041 aatgcccacc tgaaataagc aggcaaagcc tggacgcacc catgcgtccc caagaggctt
    14101 gcgcttgaaa caaaagaggc cgctcgcgcg gcctcttttg ctttcatggc caggaacggc
    14161 cgtagaaggg ccacacaggg ccccaaggcg cttcacgtgt agctcgtggg ggctgcccct
    14221 tcgaacaggg ccagccagcc cttgatcagg cgatacaggg cccacaccag cgtgatcagc
    14281 acgccgagcc agccaatgaa gatcatggtc aggatgccgc tcaggaacaa ccagagcacc
    14341 gaccaccaga aagtgcgcag cagccagtca aaatgcccgg catagaccgt gcctgccgtg
    14401 tcggagcgct tgacgtacaa gagcaccacg gccgccagcg tggcgacgcc caggatgccg
    14461 cccgtcagaa agcccagggc aaacaggccg taggccacat gcgtcaataa acgcaaatcc
    14521 ggccctgcag taggaacaga agtggtctga tcgctcacga ttgcggctcc atgaatgttt
    14581 cagggtgtcg ggtatccaca ttatcttacc gcacaaagcc aaacgcccca ggctgagtag
    14641 cctgaggcga tatgggggga taagagcctg acgatgacct actttcacag atgtccatcc
    14701 actatcatcg gcgcggtctc gtttcacggt cctgttcggg aagggaaggg gtggtaccga
    14761 gacgctatgg tcgtcaggcg tagctgggtt gaggagct
//
LOCUS       DASWPO010000005         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1161412, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000005 DASWPO010000000
VERSION     DASWPO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161412"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..661
                     /gene="leuD"
                     /locus_tag="VGC69_00155"
     CDS             56..661
                     /gene="leuD"
                     /locus_tag="VGC69_00155"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0293723.1"
                     /translation="MEPFKTLEGVAAPLNMINVDTDMIIPKQYLKTIHRTGLGKALFD
                     ELRHNPDGSEKPDFVLNKPAWRKAKILVAGDNFGCGSSREHAPWALLDFGIRCVIAPS
                     FADIFYGNCFKNGILPIKLPQEIVDKLMDDAERGANAIISIDLEKQEIRGPDGGMVTF
                     EIDPFRKQVLLNGWDDIGLTLRDEPKISSFEARQKTQTPWI"
     gene            674..1777
                     /gene="leuB"
                     /locus_tag="VGC69_00160"
     CDS             674..1777
                     /gene="leuB"
                     /locus_tag="VGC69_00160"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY0293724.1"
                     /translation="MNEYKLLLLPGDGIGPEVLDATMRVVGWYGQKGLPYQTEEALLG
                     GAAIDATGRPDPDETFEKAIAADAVLMGSVGGPKWDGLPFEKRAERGLLRVRKDMGVY
                     ANLRPALCFDALKDASTLKPEIVSGLDILIVRELMGGVYFGLPKETTTLADGQKRAVD
                     TGTYTTSEIERVCRVAFDMARLRGNKVHSAEKSNVMVTGVLWKEVVTDLHRREYSDVE
                     LHHILADNAAMQLVRNPKLFDVLVTDNLFGDVLSDEAAQLTGSIGMLPSASLGDKKAN
                     GLPSALYEPIHGSAPDIAGQGLANPIASILSFAMCLRYSFGLKNEALRLEKAVDAVLA
                     EGYRTGDIMQPGKIRVGTTAMTDAILKALDKSA"
     gene            complement(1774..2112)
                     /locus_tag="VGC69_00165"
     CDS             complement(1774..2112)
                     /locus_tag="VGC69_00165"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY0293725.1"
                     /translation="MPNGIETRLAVYGSLAPGKPNAHVLAALDGSWRPGIVRGRLVQK
                     GWGASMGFPALILDSQGDAVAVQLFESADLAAKWPQLDAFEGPGYRRVPTRVDAAGET
                     VSAYIYVLAA"
     gene            complement(2113..>2730)
                     /locus_tag="VGC69_00170"
     CDS             complement(2113..>2730)
                     /locus_tag="VGC69_00170"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049010.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY0293726.1"
                     /translation="PDRMADMLKAKIGHPLAGASCAWVPSPTAATLHALHYHQVSVTA
                     RQDELKSRPRAKLSDILTIPLADRPNWSPEEVQQELDNNAQGILGYVVRWVDQGVGCS
                     KVPDIHDVGLMEDRATLRISSQHIANWLEHGIATPEQVTETLQRMAVVVDKQNEADPF
                     YTPMAPGYGGIAFQAASDLVFKGKEQPSGYTEPLLHAARRDLKGD"
BASE COUNT          509 a          888 c          865 g          468 t
ORIGIN      
        1 cgcagggccg gggcagggtt cactttttgc cgaaaatcgc tatggtcccg gcctcatgga
       61 accgttcaaa acgcttgagg gcgtcgctgc ccctctgaac atgatcaatg tcgataccga
      121 catgatcatc cccaaacagt atctgaagac catccatcgc accggcctgg gcaaggcgct
      181 gttcgacgaa ctgcgccaca atcccgacgg cagcgagaag ccggacttcg tgctcaacaa
      241 gccggcttgg cgcaaggcca agattctggt ggcgggcgac aatttcggct gcggttcgag
      301 ccgcgaacat gccccctggg cgctgctgga tttcggcatc cgctgcgtca tcgcgcccag
      361 cttcgccgac atcttctatg gcaactgctt caagaacggc atcctgccga tcaagctgcc
      421 gcaggaaatc gtggacaagc tgatggacga tgccgaacgc ggcgccaatg ccatcatctc
      481 catcgacctg gagaagcagg aaatccgcgg tcccgacggc ggcatggtca ccttcgagat
      541 cgaccccttc cgcaagcagg tcttgctgaa cggctgggac gatatcgggc tgaccctgcg
      601 cgacgagccg aagatttcct ccttcgaggc gcggcagaaa acacagactc cctggattta
      661 ggacgcctcc acgatgaacg aatacaagct gcttctgctg ccgggcgatg gcatcggccc
      721 cgaagtgctc gacgccacca tgcgcgtggt cggctggtac ggccaaaagg gcctgcccta
      781 ccagaccgaa gaagcgctgc tgggcggcgc ggcgatcgac gccaccggcc gtcccgatcc
      841 cgacgagacc tttgaaaagg ccatcgccgc cgacgccgtg ctgatgggtt cggtgggcgg
      901 tcccaaatgg gacggcctgc ccttcgagaa gcgcgccgag cgcgggctgc tcagggtgcg
      961 caaggacatg ggcgtttacg ccaacttgcg cccggcgctg tgcttcgatg cgttgaagga
     1021 tgcctcgacc ctgaaaccgg aaatcgtctc cggtctcgat atcctgatcg tgcgcgaact
     1081 gatgggcggc gtctatttcg gcctgcccaa ggaaaccacc accctggccg acggccagaa
     1141 gcgcgcggtg gacaccggca cctataccac cagcgagatc gaacgggtct gccgcgtcgc
     1201 cttcgacatg gcgcggctgc gcggcaacaa ggtgcacagc gccgaaaaat ccaacgtcat
     1261 ggtcaccggc gtgttgtgga aggaagtcgt caccgacctg cacaggcgcg aatacagcga
     1321 cgtggaactg caccacatcc tggccgacaa tgccgccatg cagctggtgc gcaatcccaa
     1381 gctgtttgac gtgctggtca ccgacaatct gttcggcgac gtgctgtcgg acgaagcggc
     1441 gcaactgacc ggctccatcg gcatgctgcc gtcggcctcg ctgggcgaca agaaggccaa
     1501 cggcctgccg tcggcgctgt atgagccgat ccatggcagc gcgcccgaca ttgccggcca
     1561 gggcctggcc aatccgatcg cctccatcct ctccttcgcc atgtgcctgc gctattcctt
     1621 cggcctgaag aacgaggcgc tgcggctgga aaaggcggtc gatgccgtgc tggcggaagg
     1681 ctatcgcacc ggcgacatca tgcagccggg taagatccgc gtcggcacca ccgccatgac
     1741 cgacgccatc ctcaaggcgc tggacaagtc ggcctaggcc gccagcacat agatataggc
     1801 ggacacagtt tcgcccgccg catccacgcg tgtgggcacg cggcgatagc ctgggccttc
     1861 gaaggcgtcg agctgcggcc atttcgccgc cagatcggcg gattcgaaaa gctgcaccgc
     1921 gacagcgtcg ccctggctgt ccagaatgag cgcggggaag cccatgctgg cgccccagcc
     1981 tttctgcacc agccggccgc gcacgatgcc cgggcgccag ctgccatcaa gcgccgccag
     2041 aacatgcgca ttgggtttgc cgggcgccag cgagccataa accgccagcc gcgtctcaat
     2101 accgttcggc aatcagtcgc ctttgaggtc gcgccgcgcg gcatgcagca gcggttcggt
     2161 atagccgctg ggctgttcct tgcccttgaa caccagatcg ctggcggcct ggaaggcgat
     2221 gccgccatag ccgggcgcca tgggggtgta gaagggatcg gcttcattct gcttgtccac
     2281 aaccacggcc atgcgctgca gggtttcggt cacctgttcc ggcgtggcga tgccatgctc
     2341 cagccagttg gcgatatgct ggcttgagat gcgcagggtg gcgcggtctt ccatcaggcc
     2401 gacatcgtga atgtcgggca ccttggaaca gcccacgccc tgatccaccc agcgcacgac
     2461 ataacccaga ataccctggg cgttgttgtc cagctcctgc tgcacttcct ccggtgacca
     2521 gttggggcgg tcggccagcg ggatggtgag gatgtcggac agcttggcgc ggggccgcga
     2581 tttcaattcg tcctgccgcg ccgtcacgct gacctggtga tagtgcagcg catgcaatgt
     2641 ggcggcggtg ggcgagggca cccaggcgca ggaggcgccg gccaggggat ggccgatctt
     2701 ggccttcagc atgtcggcca tgcggtcggg
//
LOCUS       DASWPO010000006        12507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_269054, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000006 DASWPO010000000
VERSION     DASWPO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269054"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VGC69_00175"
     CDS             complement(<1..118)
                     /locus_tag="VGC69_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0293727.1"
                     /translation="MMEFFNDAVEYLPVLLQGVKLTILVTLGSLVLSTLLGLV"
     gene            complement(134..886)
                     /locus_tag="VGC69_00180"
     CDS             complement(134..886)
                     /locus_tag="VGC69_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0293728.1"
                     /translation="MKFKFLTIAAVMVAALASGGAQAQKVLKVGSTPTGSPFTFLDTK
                     TNTIEGVMVDITKAIGKDAGFTPQIEPMQFSTLIASLTTKRVDMISAAMFITDERKKV
                     VDFSDPIYTYGEGLVVPQGDDTPYKSFADMKGMTVGVQVGTAFVKPLQDSGMFKEVKL
                     YDTTPDLMSDVNAGRIQAAFLDFPIAAYAINQGKFPKLKMNRTYQPIIINGVGIATRK
                     DDPALLASINASLAKLKGDGTIDAILKKWGLN"
     gene            complement(938..1564)
                     /locus_tag="VGC69_00185"
     CDS             complement(938..1564)
                     /locus_tag="VGC69_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0293729.1"
                     /translation="MPAAVDVPVHAQSLVDLWLGEQLRQLRKRQRRSLADVAAVCGIS
                     LGLLSQVERGLSSPSLKVLQALAREYGVPVENLLRNAGPDKGDADGRIRRVGEHRRID
                     NPERGVYKEMYSPPASRNLELCRAVLAPGGSSGDELFATDAYEHVGVVVRGELELWLE
                     QRVMLLKAGDSFCHAGNERRRWRNPGNIETEVIWAIGRPMPGETARWS"
     gene            complement(1681..2463)
                     /gene="trmD"
                     /locus_tag="VGC69_00190"
     CDS             complement(1681..2463)
                     /gene="trmD"
                     /locus_tag="VGC69_00190"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY0293730.1"
                     /translation="MRFDAVTLFPDMFGLVRDLGVTGRAHSHAPGQGVWTMKVWNPRD
                     FTHDAHRTVDDRPYGGGPGMVMMAAPLEAAVDAAQADRAAQGLAAAPVVLLSPTGRRY
                     GQREAENLAAAGQGAIFICGRYEGVDQRFIDRRVTCEWSLGDFVLSGGEIAALAMIDA
                     AVRLLPGVLNDVDSAVQDSFNAALDGLLDSPHYTRPEVYGDVAVPEPLLSGHHARIAR
                     WRREQSLRLTAERRPDLIEQARAQGRLTREDERFLASLPAKS"
     gene            complement(2481..3095)
                     /gene="rimM"
                     /locus_tag="VGC69_00195"
     CDS             complement(2481..3095)
                     /gene="rimM"
                     /locus_tag="VGC69_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938824.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY0293731.1"
                     /translation="MSLVPADLIELGRILGAYGIKGWVKVQPHSAQAEVLRSVKQWWL
                     VRPAPQLQGGGQTAAQQPIAYPVVQARPQGATVVAQLQGIADRDQAEAARGCVIMASR
                     SAFPAPAEGEYYWIDLIGCAFYTTATDAPDAQLLGQVVEVLDNGAHAILRVQRQQAGP
                     GGELQPMLDAKERPLELLVPFVAAHIQAVDLAGRRIDSDWPADY"
     gene            complement(3107..3361)
                     /gene="rpsP"
                     /locus_tag="VGC69_00200"
     CDS             complement(3107..3361)
                     /gene="rpsP"
                     /locus_tag="VGC69_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY0293732.1"
                     /translation="MVVIRLARGGSKKRPFYNLVATDSRNRRDGRFIERVGFYNPVGN
                     EGTENLRISLDRVQYWTGNGAQLSPAVERLVKDYSAKIAA"
     gene            complement(3619..3894)
                     /locus_tag="VGC69_00205"
     CDS             complement(3619..3894)
                     /locus_tag="VGC69_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY0293733.1"
                     /translation="MSASDPPGDSSAEAGLPAWQHEVDAAGLNCPLPILRAKKALAQM
                     ASGEVLRVVTTDPNAVRDFQAFSRQTGNELLAQHWEEGSTQHFLKRR"
     gene            complement(3881..6511)
                     /gene="alaS"
                     /locus_tag="VGC69_00210"
     CDS             complement(3881..6511)
                     /gene="alaS"
                     /locus_tag="VGC69_00210"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY0293734.1"
                     /translation="MKVSEIRQKFLQFFQSKGHTIVPSSSLVPGNDPTLLFTNSGMVQ
                     FKDVFTGKESRSYTRATTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     KDAIHYAWELLTSVYKLPKEKLWVTVYHEDDEAYDIWAKEIGVPPERIIRIGDNKGAR
                     YASDNFWQMADTGPCGPCSEIFYDHGPEIWGGPPGSPEEDGDRYIEIWNLVFMQFERD
                     AAGNMPRLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFQNLIKAAARETGIQDLAENS
                     LKVIADHIRACSFLIVDGVIPSNEGRGYVLRRIVRRALRHGYKLGQTKPFFYRLVPDL
                     VAEMGEAYPELAQAAERVAQVLKQEEERFGETLENGMKILDAATAGIAKGGQLDGMTL
                     FTLYDTYGFPVDLTADICREREIEIDMPGFEAAMEHQRKQARAAGKFKMAEGLSYDGA
                     QTRFEGYEHLELSGAKVTALYVDGTQVDSVAVSKPGGRNAVVVLDATPFYAESGGQVG
                     DTGLLEGGNARFVVADTQKIQAGVFGHHGTLESGALKVGDTLLARVDVARRARTVRNH
                     SATHLMHKALRQVLGSHVQQRGSLVDPDKTRFDFAHDAPMTAEQITRVEEIVNAQVLA
                     NQATQARVMAFDDAVQDGAMALFGEKYGDTVRVLDIGFSRELCGGTHVERTGDIGLFK
                     IVSETGVAAGVRRVEAITGDNALHWAQGQNALLAKVAGQLRTPVAELPERIAQVQDQL
                     KALEKDLDQARAKLAASAGNDLADKAAEVKGIKLVAASLGEVDPKALRGMVDSLKDKL
                     KSAVVVLAAGGEGKISLAAGVTSDLTGKIKAGDLLGFVAGQVGGKGGGRPDMAMGGGT
                     DAQALPAAIASVRKWVDERL"
     gene            6659..6735
                     /locus_tag="VGC69_00215"
     tRNA            6659..6735
                     /locus_tag="VGC69_00215"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6693..6695,aa:Met,seq:cat)
     gene            complement(6947..7369)
                     /locus_tag="VGC69_00220"
     CDS             complement(6947..7369)
                     /locus_tag="VGC69_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0293735.1"
                     /translation="MVYVDTSVLVALCVNEPKSIAVAKWYAACDDELISAAWCVTEFA
                     SALGIKQRTGQIAGEQGATAWQAFERLCASDLQLWPVKPSTFHRAAVLTLDVASSLRA
                     GDALHLAAALDAKAKSMATLDDVLARNAKRVKIKPLVF"
     gene            complement(7369..7638)
                     /locus_tag="VGC69_00225"
     CDS             complement(7369..7638)
                     /locus_tag="VGC69_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0293736.1"
                     /translation="MYINTGADMNLQIAKWGNSLALRIPADYVRQAGIKEGDQVQASL
                     TIDGGICIRTAKWNRKAFAHDLEGMRAAMPMGESVMEELRHGARY"
     gene            7953..8642
                     /locus_tag="VGC69_00230"
     CDS             7953..8642
                     /locus_tag="VGC69_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0293737.1"
                     /translation="MSEFLQKIERLDLLGERVYRQLRSYLWSGNVRWGEMLRESVLAT
                     RLGVSRTPVREALMRLASEGFLEARDRGFAVPALDEEDIEDIYHLRVLLETDAVRFTA
                     SVVKRSPSLLAPIRRTIDQAALAVEKGDDEMFISANLAFRAAWLSLVKNRRLVAAVEL
                     YADLVRSLQILSLGNGQRQVIVLEGMKQICASIEKGDADAAAQTMLRYLDIARLAMLE
                     SLPAIARHNAS"
     gene            8686..9492
                     /locus_tag="VGC69_00235"
     CDS             8686..9492
                     /locus_tag="VGC69_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0293738.1"
                     /translation="MRILFQSLIEAGRTPAYFPGMQARVARIARPGVTVDFVGMPAGI
                     YGKYAPSDTVKYPYMAAYAAQLILENALRAQDEGYDVFAVGSVQDPALEEARSLVDIP
                     VVGYGESAMHFASCLGSKFVVLVFQQGFDQMMDLRVQRLGMAARALPTAVIEAGFDDI
                     GRGLSGNADALVESFAKTARRAISQGAEVLIPGQLYLSEAIVRAGVTRIDDVPVMDGL
                     SATLKMAEVMADFKQLGISVTRRGYLHAQPPRDMVEHARQFRSRADGSAP"
     gene            9513..10589
                     /locus_tag="VGC69_00240"
     CDS             9513..10589
                     /locus_tag="VGC69_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0293739.1"
                     /translation="MATTLPASPPARMPVTLVTGFLGSGKTTLVNAVLRSPGFAGSLV
                     IVNEFGDVGLDHLLIASASDQVVLLDSGCLCCAASGSLRDTLIDIYARIPSGGIPAFD
                     RIIVETSGLAHPGPLVAQLIGDSALNTRCALAQVLTLVDAQTGAATLARYAEARHQAA
                     FADRLVISKVDAAASENIDSVRQALAAINPDAALAAWHRALPAAPLFLAGEAGGQTML
                     ERPEVWLHNLMRAQPREHSPGFLQGVHDAAFGQIGSCVLRPAQAPIDWATYAHWTATF
                     NARFGPRLLRCKGLLPLGDDGRPWIVQGVQGYFAPPERLAAWPAGCERGFLVCIGDGI
                     DPAELQAVIAPGLGTPTLPSPAFS"
     gene            10602..12005
                     /locus_tag="VGC69_00245"
     CDS             10602..12005
                     /locus_tag="VGC69_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0293740.1"
                     /translation="MTNSYVLRGGTVVRGDHEPQPLDILVAGERIAALLAPGTPVAAG
                     VAERRIDGLHVFPGLVEAHMHFGFGEKITEYQTETAHAAIGGFTTVLAYFLNNEAYGE
                     VYRREQGYARPRVHTDYGFHFSTASELHLQELESYVREYGVTSFKYFMNFKGEEGRYL
                     GLDGTDDGYLYTLLGRASQIGGVTVVCHTENIELVNRLRNEQLQKGITTLREWEAIKP
                     PITEAEAAVRAMLFAEHLGATLYIPHMSSKLGLDEVRRWRQRYDRVHVETCPHYLTHT
                     SDMDLGGMGKANPPFRGRAEQDALWEGLFDGSIDVVASDHCPRKRATKDKSLWLASQG
                     FPGTATILPVLLSEGHHRRGLPLRRIAQLVCSAPARIFGIADRKGGIAPGMDADLTLV
                     DLNQERVVRHEDLLSYSDYSIYDGWTFKGWPVQTILRGKTIMQDGRLTGTPGDGRYLF
                     RNNDGTLAAPADAGAQT"
     gene            12031..>12507
                     /locus_tag="VGC69_00250"
     CDS             12031..>12507
                     /locus_tag="VGC69_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0293741.1"
                     /translation="MTTPWNDIISEAEQRAYAAAGFGRPSGLGKRPALLIIDIQYRTV
                     GTERKPFDEAIKEFTTSCGEAGWQAVDALVPLLKVFRERGWPVMYPHVAPKQAYDGGR
                     LAEKVPGIMGIPAKGYEFVAEVAPAAGDVLIPKKHPSAFFGTALCSHLIDKQVDTVI"
BASE COUNT         2088 a         4143 c         4068 g         2208 t
ORIGIN      
        1 acaccaggcc cagcagcgtc gagagcacca gggagccaag cgtcaccagg atggtgagct
       61 tgaccccttg gagcagcacg ggcagatact ccaccgcgtc attgaagaac tccatcatgg
      121 cgtgccggcg gctttagttc aggccccact tcttgagaat ggcgtcgatg gtgccgtcgc
      181 ccttgagctt ggccagggag gcattgatcg aggccagcag cgccggatcg tccttgcggg
      241 tggcgatgcc cacgccgttg atgatgatgg gctggtaggt gcggttcatc ttcagcttgg
      301 gaaacttgcc ctggttgatg gcgtaggccg cgatgggaaa gtccaggaag gccgcctgga
      361 tgcggccggc gttgacgtcg ctcatgaggt cgggcgtggt gtcgtagagc ttgacctcct
      421 tgaacatgcc gctgtcctgc agcggcttga cgaaggccgt gcccacctgc acgcccaccg
      481 tcatgccctt catgtcggcg aagctcttgt agggcgtgtc gtcgccctgg ggcaccacca
      541 ggccttcgcc ataggtgtag atggggtcgc tgaaatcgac gaccttcttg cgctcgtcgg
      601 tgatgaacat ggcagccgag atcatgtcga cgcgcttggt cgtcagcgag gcgatcagcg
      661 tcgagaactg catgggctcg atctggggcg taaagccggc gtccttgccg atggccttgg
      721 tgatgtcgac catcacgcct tcgatggtgt tggtcttggt gtccaggaag gtgaacgggc
      781 tgccggtggg cgtggagccc accttgagca ccttttgcgc ctgcgcgccg ccgctggcca
      841 gggcggccac catcacggcg gcgatcgtca gaaacttgaa cttcatgttt atccccttgc
      901 tggttgaata gtggaaatac aggaaagcca gccttgatca ggaccagcgc gcggtttcgc
      961 caggcatcgg acggccgatg gcccagatca cctcggtttc gatattgccg ggattgcgcc
     1021 agcggcggcg ctcgtttccg gcatggcaga agctgtcgcc cgccttgagc agcatcacgc
     1081 gctgctcgag ccagagttcg agctcgccgc gcacgaccac gcccacgtgc tcgtaggcgt
     1141 cggtcgcgaa caactcgtcg ccgctggatc cgccaggcgc gagcaccgcg cggcacagct
     1201 ccaggttgcg cgaggccggc gggctgtaca tttctttgta gaccccgcgc tcggggttgt
     1261 cgatgcggcg gtgctcgccg acgcgccgaa tgcgcccgtc ggcatcgccc ttgtcgggac
     1321 cggcattgcg cagcaggttt tcgaccggca cgccgtattc gcgcgccagc gcctgcagca
     1381 ccttgagcga cggcgaactc agtccgcgct cgacctggct gagcaagccc agcgagatgc
     1441 cgcacaccgc cgccacgtcg gccagcgaac gacgctgccg cttgcgcagc tggcgcagtt
     1501 gctcgcccag ccagaggtcg accaggcttt gggcatgaac ggggacgtcg actgcggccg
     1561 gcatgatgaa aatttcattt atatgtaatt ttcaatccta ggcggccgcg caacgcagca
     1621 acaagctggg agattcccta gaaaccggga gagcaacgcg gcgcatgcgc gcctcgcggt
     1681 tcaggacttc gcgggtagcg aggcgagaaa gcgctcgtct tcgcgcgtaa ggcggccctg
     1741 ggcgcgggcc tgctcgatca ggtcgggacg gcgctcggcc gtcaggcgca gggactgctc
     1801 gcgccgccag cgggcgatgc gggcatggtg gccgctgagc agcggctcgg gcacagccac
     1861 gtctccgtag acctcggggc gcgtgtagtg cgggctgtcc agcaggccgt cgagcgcggc
     1921 attgaaggag tcttgcacgg cggaatcgac gtcgttgagc acgccgggca aaaggcgcac
     1981 cgcggcgtcg atcatggcca gcgccgcgat ctcgccgccg gacagcacga agtcgcccag
     2041 cgaccattcg cacgtgacgc gccggtcgat gaagcgctgg tccacgcctt cgtagcggcc
     2101 gcagatgaaa atggcgccct gcccggccgc ggccaggttt tcggcctcgc gctggccgta
     2161 gcgccggccc gtgggcgaga gcagcacgac gggcgcggca gccaggccct gcgcggcgcg
     2221 gtcggcctgg gcggcatcga ccgcggcctc gagcggcgcg gccatcatca ccatgcccgg
     2281 gccgccgcca taaggccggt cgtccacggt gcggtgcgcg tcatgcgtga aatcgcgcgg
     2341 gttccagacc ttcatggtcc agacgccctg ccctggtgcg tggctgtgcg cccggccggt
     2401 aacccctagg tcgcggacca ggccgaacat gtcgggaaac agggtgacgg cgtcgaagcg
     2461 catcgcttag tgggtggact tcagtagtcg gcgggccagt cgctgtcgat gcggcggccg
     2521 gccagatcga cggcctggat gtgcgccgcg acaaaaggca ccagcaactc cagcgggcgc
     2581 tccttggcat cgagcatggg ctgcaactcg ccaccggggc cggcctgctg gcgctgcacg
     2641 cgcaagatgg catgcgcgcc gttgtccagc acctcgacca cctgccccag caactgggca
     2701 tcgggtgcgt ccgtggctgt ggtgtagaac gcacaaccga tcaggtcgat ccaatagtac
     2761 tcgccctcgg caggagcggg aaaggcgctg cgcgaggcca tgatcacgca accgcgcgcg
     2821 gcctcggcct ggtcgcggtc ggcgatgccc tgcagctgcg ccacgacggt ggcgccctgg
     2881 gggcgggctt gaacgacggg ataggcgatc ggttgctggg ctgcagtttg accgccgccc
     2941 tggagctgcg gcgcggggcg tacaagccac cactgcttga ccgaacgcag cacttcggcc
     3001 tgcgccgagt gcggctgaac cttcacccag cccttgattc cgtaggcccc taagatgcgg
     3061 cccagttcaa tcaggtcagc gggtacgagg ctcatgagct gccagatcag gcagcgatct
     3121 tggccgagta gtccttgacc agacgctcga cggcgggcga cagctgggcg ccgttgccgg
     3181 tccagtactg cacgcgatcc agcgagatgc gcaggttctc ggtgccttca ttgcccacgg
     3241 ggttgtagaa acccacgcgt tcgatgaaac ggccgtcgcg acggttgcgc gaatcggtag
     3301 caaccaggtt gtagaacggg cgcttcttcg agccgccgcg agccagacga atgaccacca
     3361 tggcaggtaa tcctttgaag tgaaagccta atgctagtga aagcctaatg ctttgatttt
     3421 ggaaagcaga agattctagc atttaggggg gaggcttgca atctccccct gctgtattta
     3481 ttagggtcga gcttggctct ttagccgtcg cggaccccgg agagagtccg atgctcggcg
     3541 cactgcgcct gtgcttcgga ctctctccgg ggtccgcgac tcacagccga gtgcagcccc
     3601 tacaaaactt atcgccaatt accgtcgttt gaggaagtgc tgggtgctgc cctcttccca
     3661 gtgctgggcc agcagttcgt tgccggtctg ccgggagaag gcctggaaat cgcgcacggc
     3721 attggggtcg gtggtgacca cgcgcagcac ttcgccgctg gccatctggg ccagggcctt
     3781 cttggcgcgc aggatgggca gggggcagtt cagccccgcc gcatccacct cgtgctgcca
     3841 ggccggcagg ccggcctcgg cggacgagtc gccgggcggg tcagaggcgc tcatcgaccc
     3901 acttccgcac gctggcgatg gcggccggca aggcctgggc atcggtgccg ccgcccatgg
     3961 ccatgtcggg gcgcccgccc cccttgccgc ccacctggcc ggccacgaag cccagcaggt
     4021 cgccggcctt gatcttgccc gtcaggtcgc tggtgacgcc ggcggccagg ctgatcttgc
     4081 cctcgccgcc cgcggccagc accaccacgg cggacttgag cttgtccttc aggctgtcga
     4141 ccatgccgcg cagggccttg ggatcgacct cgcccaggct ggcggccacg agcttgatgc
     4201 ccttgacctc ggcggccttg tcggccaggt cgttgccggc gctggcggcc agcttggcgc
     4261 gcgcctggtc caggtctttt tccagcgcct tgagctggtc ctgcacctgg gcgatgcgct
     4321 cgggcagctc ggccacgggc gtgcgcaact ggcccgccac cttggccagc aaggcgttct
     4381 ggccctgggc ccagtgcaag gcgttgtcgc ccgtgatggc ctcgacgcgg cgcacgccgg
     4441 cggccacgcc cgtttcggac acgatcttga acagtccgat gtcgcccgtg cgctcgacgt
     4501 gcgtgccgcc gcacagttca cgcgaaaagc cgatgtcgag cacgcgcacg gtgtcgccgt
     4561 atttctcgcc gaacaaggcc atggccccgt cctgcaccgc gtcgtcgaaa gccatgacgc
     4621 gcgcctgcgt ggcctggttg gccagcacct gggcgttgac gatttcttcg acccgggtaa
     4681 tttgctcggc cgtcatgggc gcgtcgtgcg cgaagtcgaa gcgggtcttg tcggggtcga
     4741 ccagcgagcc gcgctgctgc acatggctgc ccagcacctg gcgcagcgcc ttgtgcatga
     4801 ggtgggtggc cgagtggttg cgcacggtgc gggcgcggcg cgcgacgtcg acacgggcca
     4861 gcaaggtatc gcccactttc agcgcgcccg attcgagcgt gccgtggtgg ccgaacacgc
     4921 cggcctggat cttctgcgta tcggccacga caaagcgggc attgccgcct tccagcaggc
     4981 ccgtgtcgcc cacctggccg ccggattcgg cgtagaacgg cgtggcgtcg agcaccacca
     5041 ccgcgttccg gccgccgggt ttggaaaccg ccacgctgtc gacctgcgtc ccgtccacat
     5101 agagcgccgt gaccttggcg ccgctcagct ccaggtgctc gtagccttcg aagcgcgtct
     5161 gcgcgccgtc gtagctcagg ccctcggcca tcttgaactt gccggcggcg cgggcctgct
     5221 tgcgctggtg ttccatggcg gcctcgaagc cgggcatgtc gatctcgatc tcgcgctcgc
     5281 ggcagatgtc ggccgtcaga tccaccggga aaccataggt gtcgtacagc gtgaagagcg
     5341 tcatgccgtc gagctggccg cccttggcga tgccggccgt ggcggcgtcg aggatcttca
     5401 tgccgttttc cagtgtttcg ccgaagcgct cttcttcctg cttgagcacc tgggccacgc
     5461 gctcggccgc ctgggccagc tcgggatagg cctcgcccat ttcggccacc aggtcgggca
     5521 ccaaacggta gaaaaagggc ttggtctggc ccagcttgta gccgtggcgc agggcgcggc
     5581 ggacgatgcg gcgcagcaca tagccgcggc cttcattgct ggggatgacg ccgtcgacaa
     5641 tcagaaacga acaggcgcgg atgtggtcgg cgatgacctt gagcgaattc tcggccagat
     5701 cctggatgcc ggtttcgcgg gcggcggcct tgatcaggtt ctggaaaagg tcgatctcgt
     5761 agttggagtg cacgtgctgc agcacggccg cgatgcgctc caggcccatg ccggtgtcca
     5821 cgcagggctt gggcaggcgc ggcatgttgc cggcggcgtc gcgctcgaac tgcatgaaca
     5881 ccaggttcca gatctcgatg tagcggtcgc cgtcttcctc gggcgaaccg ggggggccgc
     5941 cccagatctc gggtccgtgg tcgtagaaga tttccgagca ggggccgcag gggccggtgt
     6001 cggccatctg ccagaagttg tcggacgcgt agcgggcgcc cttgttgtcg ccgatgcgga
     6061 tgatgcgctc gggcggcacg ccgatttcct tggcccagat gtcataggcc tcgtcgtctt
     6121 cgtggtagac ggtcacccac agcttttcct tgggcagctt gtagaccgag gtcaacagct
     6181 cccaggcgta gtggatggcg tctttcttga agtagtcgcc gaagctgaaa ttgcccagca
     6241 tctcgaagaa agtgtggtgg cgcgcggtat agccgacgtt ttccaggtcg ttgtgcttgc
     6301 cgccggcgcg cacgctgcgc tgcgccgtgg tggcgcgcgt gtagctgcga gattccttgc
     6361 cggtaaagac gtccttgaac tgcaccatgc ccgagttggt gaagagcagc gtggggtcgt
     6421 tgcctggcac cagcgacgag gacggcacga tggtgtgtcc cttggactgg aagaactgca
     6481 ggaatttctg gcgaatctca gagactttca tcgtgaaatg atgggggatt tgagcgaatc
     6541 ttccatttta gctcagggta ggcaggggaa gggacacaac cgcttcgcct tggaatttgc
     6601 gcctgatacg agacccatgc tagaattttt ttcttcgctt tgtacgcaca aagcgtgggg
     6661 cgcattagct cagccggtta gagcgacgga atcataatcc gcaggtcccc tgttcgaatc
     6721 agggatgcgc caccaaagaa tatcaagcgc ttaggccaac tcttcggggt tggccttttg
     6781 cttttctgcg gctatgtacc ccacagtggt cgatacaaga tgttccccac agccagccaa
     6841 gaatcctctt gtacaggtac gttgtggtca ggcggtcttg gtgctggaac accctggccg
     6901 ccgcttcgcc tgattactgc tccgtcaggc gtcggtcatg ctcggttcaa aacaccagcg
     6961 gctttatctt cacgcgcttt gcattacgcg ccagtacgtc atcgagcgtt gccatgctct
     7021 tggccttggc atcgagtgcc gccgccaggt gtagtgcatc ccccgctcgc agactgctgg
     7081 ccacatccaa cgtcagcacc gccgcacggt ggaacgtgga cggtttgact ggccacagtt
     7141 gcaggtcact ggcgcaaagt cgctcgaacg cctgccatgc cgtcgcgccc tgctcgccgg
     7201 caatctgtcc cgtcctctgc ttgataccca aggcgctggc aaattcggtc acgcaccacg
     7261 cagcagaaat aagctcgtcg tcacaagcgg cataccactt cgcgacggcg atgctcttcg
     7321 gttcgttcac gcacagcgcg accagcacgc tggtgtccac atacaccatc agtagcgtgc
     7381 cccatgccgc aattcttcca ttacggactc gcccatcggc atggcggctc gcattccttc
     7441 aaggtcatgc gcaaaggctt tgcggttcca tttggccgtg cgaatgcaga taccgccgtc
     7501 aatggtcagg ctggcctgca cctggtcgcc ttccttgatg cccgcttggc gcacatagtc
     7561 ggcgggaatg cgtagggcaa ggctgttgcc ccatttagct atttgcaggt tcatatccgc
     7621 ccccgtattg atatacattg atgtttatac attaaagcgc cttatttgac ggctcgcaac
     7681 cccgactcgg cctgctccgg ctgaaattcg acgccagcct ccagccatcc aagcctcaat
     7741 cttggcgtac cacacacatg cgcagcagat ccagggggcg gcaacgagcg ccttattgcg
     7801 ggcgatgcga agctctttca tacggccatc agccgcggtc ttgctggcga atatccctat
     7861 cgttttcccg aatactcgcg cctattgatc gacggcatcc tgaacacggt atacagtact
     7921 tcgtcgacct aactcagtat tcctttcctg ccatgtctga attcctgcaa aagatcgagc
     7981 ggctcgacct gctcggcgaa cgtgtctacc gtcaattgcg ctcgtacctc tggtcgggca
     8041 acgtgcgatg gggcgaaatg ctgcgggaaa gcgtgctggc cacccgcctg ggcgtgtctc
     8101 gcacgccggt gcgcgaggcc ctgatgcggc tggccagcga aggcttcctg gaagcgcgcg
     8161 accgcggctt tgccgtgccg gcgctcgatg aagaagacat cgaggacatc tatcacctgc
     8221 gggtgctgct cgaaaccgac gccgtccggt tcaccgcgtc ggtcgtcaag cgctcgcctt
     8281 cgctgctggc gccgatccgg cgcacgatcg accaggccgc cctggccgtc gaaaaaggcg
     8341 acgacgaaat gttcatctcg gccaacctgg ccttccgggc ggcatggctg agcctggtaa
     8401 aaaaccgccg cctggtcgcc gccgtggagc tctatgcgga cctggtgcgc tcgctgcaga
     8461 tactgagcct gggcaacggc cagcggcagg tcatcgtgct cgaaggcatg aagcagattt
     8521 gcgcaagcat cgagaaaggc gacgccgacg cggcggcgca gaccatgctg cgctacctgg
     8581 acatcgcccg cctggccatg ctggaaagcc tgccggccat cgcgcggcac aacgcctcat
     8641 agcaaacctt cggctttgac gggaccacca cagcggagca tggccatgcg catactgttt
     8701 cagagcctca tcgaagccgg acgaacgccc gcctatttcc cgggcatgca ggccagggtg
     8761 gcgcggatcg cccgccccgg cgtcacggtg gatttcgtcg gcatgcccgc gggcatctac
     8821 ggcaagtacg cgccctcgga cacggtcaag tatccctaca tggcggccta cgctgcgcag
     8881 ctcatcctgg agaacgcgct gcgcgcgcag gacgagggct atgacgtgtt cgccgtgggc
     8941 tcggtgcagg acccggcgct ggaggaagcc cgctcgctgg tggacatccc tgtggtggga
     9001 tacggcgaat cggccatgca cttcgcctcc tgcctgggca gcaagttcgt cgtgctcgtt
     9061 ttccagcagg gcttcgacca gatgatggac ctgcgcgtgc agcgcctggg catggcggcg
     9121 cgggcgctgc ccacggccgt catcgaggcc ggcttcgatg acatcggccg tggactctcc
     9181 ggaaacgccg acgccctggt ggaatccttc gccaagacag ctcggcgggc catttcccag
     9241 ggcgccgagg tattgatacc gggccagttg tatctcagcg aagccatcgt gcgcgccggc
     9301 gtcacgcgca tcgacgacgt gccggtcatg gacggcctga gcgcaacgct gaaaatggcc
     9361 gaggtgatgg ccgacttcaa gcagcttggc atcagcgtca cgcgccgggg ctacctgcat
     9421 gcccagccgc cgcgcgacat ggtggagcac gcgcgccagt tccgcagccg cgccgacggt
     9481 tcggcgccct gacccgccgc ggccccacgc acatggcgac tacgctgccc gcctctcccc
     9541 ccgcccgcat gccggtgacg ctggtcacgg gctttctagg cagcggcaag accacgctgg
     9601 tcaacgcggt gctgcgctcg ccggggttcg ccggctcgct ggtcatcgtc aatgaattcg
     9661 gcgacgtggg gctggatcac ctgctgatag ccagcgcaag cgaccaagtc gtactgctgg
     9721 actcgggctg cctgtgctgc gcggcctcgg gcagcctgcg cgacacgctc atcgacatct
     9781 acgcccgcat cccgagcggc ggcatcccgg ccttcgaccg catcatcgtc gagaccagcg
     9841 ggctcgccca cccagggccg ctggtggcgc agctcatcgg cgactccgcc ctgaacacgc
     9901 gctgcgcact ggcccaggta ttgacactgg tcgatgcgca gaccggcgcc gcaacgctcg
     9961 cgcgatacgc cgaagcgcgg caccaggctg cattcgcaga ccggctcgtg atcagcaagg
    10021 tcgatgcggc cgcctccgag aatatcgact cggttcgaca ggcgctggcc gctataaacc
    10081 ccgacgccgc acttgcggcc tggcatcggg cgctgccggc cgcgccgctg ttcctcgccg
    10141 gcgaggccgg cggccaaacc atgctggagc gtccggaagt ctggctgcac aacctgatgc
    10201 gcgcacagcc gcgcgaacat tcgccgggat tcctgcaagg cgtgcacgac gcggctttcg
    10261 gccagatcgg cagttgcgtg ctgcgccccg cgcaagcccc gatcgactgg gccacctatg
    10321 cgcactggac ggccacgttc aatgcccggt tcggcccacg gctgctgcgc tgcaagggcc
    10381 tgctgccctt gggcgatgac ggccggccct ggatcgtgca aggcgtccag ggctatttcg
    10441 ccccgcccga acgcctggcc gcctggcccg ccggctgcga gcgcggcttt ctggtgtgca
    10501 taggcgacgg catcgatccc gccgaactgc aagccgtcat cgcgccgggc ctcggcacgc
    10561 cgacccttcc ctctcctgct ttctcctgac cgacatcgac catgaccaac agttatgtac
    10621 tccgcggcgg caccgtcgtg cgcggcgacc atgaacccca gccgctcgac attctggtgg
    10681 cgggcgagcg catcgccgcg ctgctggcgc ccggcacgcc cgtggcggcc ggcgttgccg
    10741 agcgccgcat cgacggcctg cacgtctttc caggcctggt cgaggcgcac atgcacttcg
    10801 gcttcggcga aaaaatcacc gaataccaga ccgagacggc gcacgcagcc atcggcggat
    10861 tcaccaccgt gctggcgtat ttcctgaaca acgaagccta cggcgaggtc tatcgccgcg
    10921 aacagggcta tgcccggccg cgcgtgcaca ccgattacgg cttccacttc agcacggcct
    10981 ccgagctgca cctgcaggag ctggagtcgt acgtgcggga gtacggcgtc acctcgttca
    11041 agtacttcat gaatttcaaa ggcgaggaag gccgctacct gggcctggac gggacggacg
    11101 acggctatct ctacacgctg ctgggccgcg cgtcgcagat cggcggcgtg accgtggtgt
    11161 gccataccga gaacatcgag ctggtcaacc ggctgcgcaa cgaacaactg caaaaaggca
    11221 tcaccaccct gcgcgaatgg gaagccatca agccgcccat caccgaggcc gaagctgccg
    11281 tgcgcgccat gctgttcgcc gagcacctgg gggccaccct ctacatcccg cacatgagct
    11341 cgaagctggg cctggacgaa gtgcgccgct ggcgccagcg ctacgaccgc gtgcatgtgg
    11401 agacctgccc gcactacctc acgcacacca gcgacatgga cctcggcggc atgggcaagg
    11461 ccaatccgcc ctttcgcggc cgcgccgagc aggatgcgct gtgggaaggc ctcttcgacg
    11521 gcagcatcga cgtcgtggcc tcggaccact gcccgcgcaa gcgggccacg aaagacaagt
    11581 cgctatggct ggcctcgcag ggattccccg gcaccgccac catcctgccc gtgctgctca
    11641 gcgaggggca tcaccggcgc ggcctgccgc tgcggcgcat tgcgcagctg gtgtgctcgg
    11701 cgcccgcccg catcttcggc atcgccgacc gcaagggcgg catcgcgccc ggcatggacg
    11761 cggatctgac gctggtggac ctgaaccagg aacgcgtggt gcggcacgag gacctgcttt
    11821 cgtattcgga ctacagcatc tacgacggct ggaccttcaa gggctggccg gtgcagacca
    11881 tcctgcgcgg caagaccatc atgcaggacg gccggctgac gggcacgccg ggcgacgggc
    11941 gatacctctt tcgcaacaac gacggcacgc tggcggcccc cgcggacgcc ggcgcgcaaa
    12001 cctgacctat acgaccacgg agcatccatc atgacgacgc cttggaacga catcatcagc
    12061 gaagccgagc aacgcgccta tgccgccgcc ggcttcggca gacccagcgg actcggcaag
    12121 cggcccgccc tgctcatcat cgacatccag taccggaccg tcggcaccga gcgcaagccc
    12181 ttcgacgaag ccatcaagga attcaccacc tcgtgcgggg aagccggctg gcaggcggtc
    12241 gacgcactgg tcccgctgct caaggtgttc cgggagcgcg gctggcccgt gatgtacccg
    12301 cacgtcgcgc ccaagcaggc ctacgacggc ggccggctgg ccgagaaagt gcccggcatc
    12361 atgggcatcc cggccaaggg ctacgaattc gtcgcggaag tagcgcccgc agcaggcgac
    12421 gtgctcatcc ccaagaagca ccccagcgcg ttcttcggca ccgccttgtg cagccacctg
    12481 atcgacaagc aggtcgacac cgtcatc
//
LOCUS       DASWPO010000007         6087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2591028, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000007 DASWPO010000000
VERSION     DASWPO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591028"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1424
                     /locus_tag="VGC69_00255"
     CDS             120..1424
                     /locus_tag="VGC69_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0293742.1"
                     /translation="MDQTLQGTAAPAISLSEEDRIYRKVSWRLVPFLILCYVVAYLDR
                     VNVGFAKLQMLQDLNLSDAVYGLGAGIFFIGYFIFEIPSNVILHRVGAKLWIARIMIT
                     WGLVSAAMMFTNSATTFYVLRFLLGAAEAGFFPGIILYLTYWYPNARRGRMTTLFMVA
                     IPLSGLIGGPLSGWIMSSVSSHGLAGWQWLYLLEGIPSVIVGIAVLFFLDDRIEKAKW
                     LTDSEKAVLSRNIAAEDQVKEDPPITAAMGMPRVWAFALTYFCFVMGLYGVGFWMPTL
                     IKNTGVSSTLEIGLLTAIPNVFAVIGMILIARSSDRAHERRWHLAIPAVFAALGLVWT
                     TMAVNVPAAMIALTVANIGICAVLPLFWSQPTAILSGTAAAAGIALINSVGNLAGFVS
                     PYLVGWLKDSTGSTNAGMYLLAGSLVVGGLIALSQPARLVNK"
     gene            complement(1668..3383)
                     /gene="recJ"
                     /locus_tag="VGC69_00260"
     CDS             complement(1668..3383)
                     /gene="recJ"
                     /locus_tag="VGC69_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY0293743.1"
                     /translation="MVSPRLTVRPADANARRALEAAGIHPLLSRLWAARGVTHPDQTR
                     LAWPTMLPPSQLTQAGHAAAVLADAIQQGRRLLIVADYDCDGATACAVGLRALAAMGA
                     NVDFLVPNRFETGYGLSPAVVELALRHANGKPDLIITVDNGIASVDGVAAANAAGIGV
                     VVTDHHLPGDTLPAALAIVNPNQPGCGFPSKNLAGVGVIFYLMLALRAELRRRGVYPP
                     DGGPRLDALSDLVALGTVADVVKLDANNRLLVTQGLARMRSGRLQPGLRALFAVASRE
                     PREASSFDLGFALGPRINAAGRLADMSLGIACLTTDDEAQALDLARQLDQINRERRGI
                     EAEMRDQAMAAMAAELESSADAALGACVCVYDDSWHQGVVGLVASRLKEKFWRPTLAF
                     APAGDDEIRGSGRSIPDVHLRDALDLVSKRNPGLIRKFGGHAMAAGLTLGRGDFPTFS
                     SAFDAAVRELTGRDAFEPLIETDGSLESGYANADVAGLLQQQVWGAGFAAPLFLDEFY
                     VRSQRLVGGKHMKLSLERGQQRFDAIWFNHDQPSPERIQAAYRLEQNVWNGMVNVQLV
                     IEHAE"
     gene            complement(3365..4315)
                     /locus_tag="VGC69_00265"
     CDS             complement(3365..4315)
                     /locus_tag="VGC69_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293744.1"
                     /translation="MLIVLPGALPDSSVASELAKHLPQRAPTLHAWLLMAQAQAESCD
                     LRVRGCTPYEAWQLEQAGYTAQPGLPWGAGLGPLLAGEACREGEAVWLGELAHLALGT
                     DRATLLSPDLMDLRAAESQILFEAAQPVFQDSGFTAEPLAPNRWRLRLPDGLRPETAS
                     PQAVAGQPLDAWWQHDAGTRPWRRLLNEIQMAWYEHPVNEARAARGVAPVNALWLYGG
                     ATPWPAPKLARPAQVLDALLAPQREGDWAAWLDALAELDAKRLAPLAQGKYRLPAPHI
                     EVLLLGTDRQVRLGFKPRAGLLRWLPPVKYNWNTWWSRPV"
     gene            4451..5686
                     /locus_tag="VGC69_00270"
     CDS             4451..5686
                     /locus_tag="VGC69_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY0293745.1"
                     /translation="MARWWGKNSKRGDRFVSFIAASSMAGIALGVAALIVVLSVMNGF
                     QKDVRDRMLSVLPHIELYVPGAAPEQVLAQWQRFADLAKENPAVRGTAPFVAAQAMLV
                     RGQALSGVQVRGIDPALEGNVSDLPKQMVAGKLDDLKPGSFGLVLGSDLADTLRVKVG
                     DTVLMLAPQGSISPAGFAPRMRQFTVAGIFSSGDYQYDSSLAFVDTDDAARVFRDSGT
                     AGVRLRVADMQRAPEVAAELSRTLPSYVIPSDWSRNNRTWFAAVQTEKRMMFLILALI
                     VAVAAFNLLSSLVMAVKDKQSDIAILRTLGAGPGEVARIFLVQGALIGVLGTLAGVLG
                     GMLLAYNIETVVPFIERLFGVHFLPREVYFISELPSDPQARDIITIGLTSLVLSLLAT
                     LYPSWRASRLQPAQVLRHD"
     gene            5679..>6087
                     /locus_tag="VGC69_00275"
     CDS             5679..>6087
                     /locus_tag="VGC69_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0293746.1"
                     /translation="MTDAATSAAGSTTPALQAEHLTKFYDEGPARIDVLTDVSLTVAR
                     GEMVSIIGASGSGKSTLLHILGLLDVPTEGSVTVDGTAAAGLSETAKSALRNRSLGFV
                     YQFHHLLPEFSALDNVAMPLIVRRQSRAAARAAA"
BASE COUNT          893 a         1937 c         2251 g         1006 t
ORIGIN      
        1 taggctcctg acagaattgc ctatcctgaa aaatatcagg gacgcaattc acgaagcctc
       61 gggcttcggc ccgcgcaccc gaccggtgcg caggatcatt cataaatatg gagacagcaa
      121 tggatcaaac cttgcagggc acggccgcgc cggccatttc actttccgag gaggaccgca
      181 tctaccgcaa ggtgagctgg cgcctcgttc ccttcttgat actgtgttac gtggtggcct
      241 acctcgaccg ggtgaacgtg ggctttgcca agctgcagat gctgcaggac ctgaacctga
      301 gcgacgcggt ctacggactg ggcgcgggga tcttcttcat cgggtatttc attttcgaga
      361 ttcccagcaa tgtgatcctg caccgggtgg gcgccaagct gtggattgcc cgcatcatga
      421 ttacctgggg cctggtctcg gcggccatga tgttcaccaa ttcggcgacc accttctatg
      481 tgctgcgctt cctgctgggc gcggcggagg ccggcttctt tccggggatc atcctctacc
      541 tgacctactg gtaccccaat gcccgccgcg gccggatgac gaccctgttc atggtggcga
      601 tcccgctgtc gggcctgatc ggcgggccgc tgtcgggctg gatcatgtcg tcggtcagca
      661 gccacggcct ggccggctgg cagtggctgt acctgctgga ggggattcct tcggtcatcg
      721 tgggcatcgc ggtgctgttc tttctggatg accgcatcga gaaagccaag tggctgaccg
      781 acagcgagaa ggcggtgctg agccgcaata tcgcggccga agaccaggtg aaggaagatc
      841 cgccgatcac ggccgcgatg ggcatgccgc gcgtgtgggc gttcgcgctg acctatttct
      901 gcttcgtgat gggcctgtac ggggtggggt tctggatgcc gacccttatc aagaacacgg
      961 gggtgtcgag caccctggag atcggcctgc tgacggcgat tccgaatgtg ttcgcggtga
     1021 tcggcatgat cctgatcgcg cgcagttcgg accgcgccca cgagcgccgc tggcatttgg
     1081 cgattccggc ggtgtttgcg gcgctggggc tggtctggac gacgatggcg gtcaatgtgc
     1141 cggcggcgat gatcgcgctg accgtggcca atatcggcat ctgcgcggtt ctgcccttgt
     1201 tctggagcca gccgacggcc atcctgagcg gcacggcggc ggcggcgggt attgcgctga
     1261 tcaattccgt gggcaacctg gcgggctttg tcagcccgta tctggtgggc tggctgaagg
     1321 attccacggg gtcgaccaac gcggggatgt acctgttggc gggcagcctg gtggtcggcg
     1381 ggttgatcgc tttgtcgcag cctgcgcggt tggtgaataa ataagcggct ggcgcttgcc
     1441 cgcttttcag ggtccccgcg ccgcctcccg acggactcac ggcaacgcga aggcgtagca
     1501 ggcttggaga tgtcgcaagc attggtgccg gggcatgggg tgtgcggatg cgttgagcgg
     1561 cgggcagccc ccgcaggggg ccgagggcgc ctgcatgcgc ccgagccgcc gcgatgcatc
     1621 cgcgcacccc atgccccggc accaatgcgg ctcaagaact caatacgcta ttccgcgtgc
     1681 tcaatcacca actgcacgtt caccatcccg ttccacacgt tctgctccag ccggtaagcc
     1741 gcctgaatgc gctcaggcga gggctggtcg tggttgaacc agatggcgtc gaagcgctgc
     1801 tggccgcgtt ccagggagag cttcatgtgc ttgccgccga ccaggcgctg gctgcgcaca
     1861 tagaactcgt cgagaaagag cggggcggcg aagccggcgc cccagacctg ctgctgcagt
     1921 agcccggcga cgtcggcgtt ggcgtagccg gattcgagcg agccgtcggt ctcgatcagc
     1981 ggctcgaaag cgtcgcggcc ggtgagttcg cgcacggcgg cgtcgaaggc gctggagaag
     2041 gtggggaaat cgcctcggcc cagggtgagg ccggccgcca tggcgtggcc gccgaacttg
     2101 cggatgaggc cggggttgcg cttggagacg aggtccagcg catcgcgcaa gtgcacgtcg
     2161 ggaatggagc ggccggagcc gcggatttcg tcgtcgccgg cgggcgcgaa ggccagggtg
     2221 gggcgccaga atttttcttt caggcgcgag gccaccaggc cgaccacgcc ctggtgccag
     2281 gaatcgtcgt agacgcagac gcaggcgccc agcgccgcat cggcgctgct ttccaactcc
     2341 gcggccatgg cggccatggc ctggtcgcgc atctcggcct cgatgccgcg gcgttcgcgg
     2401 ttgatctggt cgagctggcg ggccaggtcc agggcctgcg cctcgtcgtc ggtggtgagg
     2461 caggcgatgc ccaggctcat gtcggccagg cgcccggcgg cgttgatgcg cgggcccagg
     2521 gcaaagccca gatcgaagct gctggcttcg cgcggttcgc gcgaggccac ggcgaagagc
     2581 gcgcgcaggc cgggctgcag gcggccgctg cgcatgcgcg ccaggccctg ggtgacgagc
     2641 aggcggttgt tggcgtcgag cttgacgacg tcggccacgg tgcccagggc gaccaggtcg
     2701 gacagcgcgt ccaggcgcgg gccgccgtcg ggcggataga cgccgcggcg gcgcagctcg
     2761 gcgcgcaggg ccagcatgag gtagaagatc acgcccacgc cggccaggtt cttggacgga
     2821 aagccgcagc cgggctggtt ggggttgacg atggccagcg ccgcggggag cgtgtcgccg
     2881 ggcaggtggt ggtcggtgac gaccacgccg atgccggcgg cgttggccgc ggccacgccg
     2941 tcgacgctgg cgatgccgtt gtcgacggtg atgatgaggt cgggcttgcc gttggcgtgg
     3001 cgcagggcca gttcgacgac ggcgggcgac aggccgtagc cggtctcgaa gcggttgggc
     3061 accaggaagt ccacgttcgc gcccatggcg gccagggcgc gcaggccgac ggcgcaggcg
     3121 gtggcgccgt cgcagtcgta gtcggcgacg atgagcaggc gcctgccttg ctggatcgca
     3181 tcggccagca cggcggcggc gtggccggcc tgggtgagct ggctgggcgg cagcatggtt
     3241 ggccaggcca ggcgggtctg gtcgggatgg gtgacgccgc gggcggccca caggcgcgag
     3301 agcagcggat ggatgccggc agcttccagc gcgcggcggg cattggcatc ggcaggacgg
     3361 acggttagac ggggcgagac caccaggtat tccagttgta tttgacgggg ggcagccagc
     3421 gcagcaggcc ggcgcgcggt ttgaagccca gccgcacctg gcgatcggtg cccagcagca
     3481 gcacctcgat gtggggggcg ggcaggcggt atttgccctg ggccagcgga gccaggcgct
     3541 tcgcgtcgag ttcggccagg gcgtcgagcc aggcggccca atcgccttcg cgctggggag
     3601 ccagcagggc gtccagcacc tgggcgggcc gggccagctt gggcgcgggc cagggcgtgg
     3661 cgccgccgta gagccacagc gcgttgacgg gcgccacgcc gcgggcggcg cgggcttcgt
     3721 tgacggggtg ttcgtaccag gccatctgga tttcgttgag caggcggcgc caggggcgcg
     3781 tgccggcgtc gtgctgccac caggcgtcca gcggctggcc ggcgacggcc tgggggctgg
     3841 cggtttcggg gcgcaggccg tcgggcaggc gcaggcgcca gcggttcggc gccagcggtt
     3901 cggccgtaaa gccgctgtcc tggaagacag gctgggcggc ttcgaacagg atctggcttt
     3961 ccgcggcgcg caggtccatc agatcgggag acagcagggt ggcgcggtcg gtgcccaggg
     4021 ccaggtgggc cagctcgccc agccagacgg cctcgccctc gcggcaggcc tcgccggcca
     4081 gcagggggcc gaggccggcg ccccagggca ggccgggctg ggcggtgtag ccggcctgtt
     4141 ccagttgcca ggcctcgtag ggggtgcagc cccggacgcg caggtcgcag gactcggcct
     4201 gagcctgggc catcagcagc caggcatgca gggtcggcgc ccgttgaggc aggtgcttgg
     4261 ccagttcgct ggcgacggag gagtcgggca gggcgcccgg aagcacgatg agcatggcgg
     4321 gattgtagtc gcctcgccaa gactgattca gtccgcttca gggtttggga ttgccggcgc
     4381 aaatatgcgc aataatgcgc acgtgctcaa aataccttac gaattctgga taggcgcccg
     4441 ctatgcgggc atggcccgct ggtggggcaa aaacagcaag cggggcgatc gcttcgtttc
     4501 cttcatcgcg gcctccagca tggccggcat cgccctgggc gtggccgcgc tgatcgtggt
     4561 gctgtcggtg atgaacggct ttcagaaaga tgtgcgcgac cgcatgctgt cggtgctgcc
     4621 gcacatcgag ctctacgtcc ccggcgctgc gcccgagcag gtgctggcgc agtggcagcg
     4681 gttcgccgac ctcgccaagg aaaaccccgc ggtgcgcggc accgcgccct tcgtggcggc
     4741 ccaggccatg ctggtgcgcg gccaggccct gagcggcgtg caggtgcgcg gcatcgatcc
     4801 cgccctcgaa ggcaatgtgt cggatcttcc caagcagatg gtcgccggca agctcgacga
     4861 cctcaagccg ggcagcttcg gcctggtgct gggcagcgac ctggccgaca ccctgagggt
     4921 gaaggtcggc gacacggtgc tgatgctggc gccgcagggc agcatcagcc cggcgggctt
     4981 cgcgccgcgc atgcggcagt tcaccgtggc gggcattttt tcttccggcg actaccaata
     5041 cgactcgtcg ctggccttcg tggacaccga cgacgcggcg cgcgtgttcc gcgacagcgg
     5101 cacggccggc gtgcggctgc gcgtggccga tatgcagcgc gcgcccgagg tggccgccga
     5161 gctgtcgcgc acgctgccgt cctacgtcat ccccagcgac tggtcgcgca acaaccgcac
     5221 ctggtttgcc gcggtgcaga ccgaaaagcg catgatgttc ctgatcctgg cgctgatcgt
     5281 ggcggtggcg gccttcaacc tgctgtcctc gctggtgatg gccgtcaagg acaagcaatc
     5341 cgacatcgcc atcctgcgca cgctgggcgc cggtccgggc gaggtggcgc ggatcttcct
     5401 ggtgcagggc gcgctgatcg gcgtgctggg cacgctggcg ggcgtgctgg gcggcatgct
     5461 gctggcctac aacatcgaaa ccgtcgtgcc tttcatcgaa cgcctgtttg gcgtgcactt
     5521 cctgccgcgc gaggtgtatt tcatcagcga actgccctcc gacccgcagg cgcgggacat
     5581 catcaccatc ggcctgacct cgctggtgct gtcgctgctg gccacgcttt accccagctg
     5641 gcgcgcctcg cgcctgcaac ccgcccaggt gctgcgccat gactgacgcc gccacgtccg
     5701 cagccgggtc cacaacgccg gcgctccagg ccgagcacct gaccaagttc tacgacgagg
     5761 gcccggcgcg catcgacgtg ctcacggacg tgtcgctgac ggtggcgcgc ggcgagatgg
     5821 tgtccatcat cggcgcctcc ggctcgggca agagcacgct gctgcacatc ctgggcctgc
     5881 tggacgtgcc caccgagggc agcgtgacgg tcgacggcac ggccgcggcc ggcctgagcg
     5941 agaccgccaa gagcgcgctg cgcaaccgca gcctgggttt cgtctaccag ttccaccact
     6001 tgctgccgga attctcggcg ctggacaacg tggccatgcc cctcatcgtg cggcggcaaa
     6061 gccgcgccgc cgcgcgcgcg gcggcgc
//
LOCUS       DASWPO010000008        13514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_270383, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000008 DASWPO010000000
VERSION     DASWPO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13514
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270383"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /gene="clpB"
                     /locus_tag="VGC69_00280"
     CDS             complement(<1..2336)
                     /gene="clpB"
                     /locus_tag="VGC69_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY0293747.1"
                     /translation="MRFDKLTTKFQQALADAQSLAARNDHPYIEPVHVLAALLGDADS
                     GASSLLARSGVAVNRVQAALDAAIKNLPQVQGGGEVQVGRELQGVFTRVDKEAARRGD
                     TYIASELFLLALADDKGDAGRILRDAGLQKKALETAIDAVRGGESVSAAEGESNREAL
                     AKYTIDLTERARLGKLDPVIGRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVNDEVPESLRGKRVLSLDLAALLAGAKFRGEFEERLKAVLKELSQDEGQDIVFI
                     DEIHTMVGAGRAEGAMDAGNMLKPALSRGELHCIGATTLDEYRKYIEKDAALERRFQK
                     VLVDEPDVESTIAILRGLQERYEIHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDL
                     IDEAAARIRMEIDSKPEVMDKLDRRIIQLKIEREAVKKETDDASKRRLAVIEEELEKL
                     QREYNDYEEIWKAEKAAVQGTQVIKEEIDRVRAEMAEMQRKGQFDKLAELQYGKLPEL
                     EARLKAADKAEQQAGGAEAGKPRLLRTQVGAEEIAEVVSRATGIPVSKMMQGERDKLL
                     KMEDFLHRRVVGQDEAVHLVSDAIRRSRAGLSDPSRPLGSFLFLGPTGVGKTELTRAL
                     ADFLFDSEDHMVRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRKPYSVILL
                     DEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNVLIIMTSNLGSEFLAMQKDGEDV
                     EEARDEVMGVVRAHFRPEFLNRIDEIILFHRLRREDMGA"
     gene            2459..2872
                     /locus_tag="VGC69_00285"
     CDS             2459..2872
                     /locus_tag="VGC69_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEY0293748.1"
                     /translation="MSLTIFVYGTLRGGEANDLALAAARAGLPPPQALGPARVPGRLV
                     DFGDWPGLLPDGPGTVLGDLYAVDPSLVALMDAIEEYTPDAPCCFVRRPVQARTAAGP
                     VDCQYYPIDPAFRGQARDIPGDDWIAWRRQRRHQA"
     gene            2926..4101
                     /locus_tag="VGC69_00290"
     CDS             2926..4101
                     /locus_tag="VGC69_00290"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0293749.1"
                     /translation="MTLANASPATAAASLTALETPALVLDAERMQANARMMLDRARSL
                     GVALRPHVKTAKCLEVALAACGGKPGPITVSTLKEAEQFFAAGYTDILYAVGIVPGKL
                     AHVARLRRAGCDLKILLDNAAVAQAVAQARKTEAVDLPTLLEIDSDGHRSGLHPADPA
                     LAAIAGQLRAGGVTVAGVMTHAGESYNCGSLAEIEAMAEQERAACVQAAQRLRAAGHA
                     CPIVSVGSTPTARYARNLQGVTELRAGVYIFFDLVMAGLGVCTLDEIALSVLVTVLGH
                     QPDRGWILTDGGWMAMSRDRGTARQAEDQGYGAVCDLAGRPIAGLKMRDANQEHGILA
                     FDAAQGSPDPQAAFPIGTLLRILPNHACATGAQHGAYQVVHGDSPHIDAVWPRFSGW"
     gene            4112..5323
                     /locus_tag="VGC69_00295"
     CDS             4112..5323
                     /locus_tag="VGC69_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293750.1"
                     /translation="MNLRARYRRLPGLWAMLLLAASASSAAQPPPAPADPYADPAPAP
                     ARSAPAWQRLELGAKGHRYAFPVYASQRLNAPGALRKVQRLVIVLHDERREAERSLEA
                     ITALYAGQAEHDADTLVIAPRFPGLVDAGFGSMPAWRRTGWMEGQPSAAAQGRPAPVG
                     AFQVIDDLLRELTAPGRLPALHAIVVAGHGAGAQMAQRYAVLNPLDESLRATGLALSY
                     VVANAESYLYLSPERPNRNGRGYTRYERGICPTYDQYRYGLESMPAALDAYQGKLDHA
                     RLAARYAQRRVVYLMGGADNNPEYPGLDKGCGAEAQGATPLARGLGYWGYEIRTPPHG
                     APAARAGGAARPSHQGFIAMGAGHSETDLYASACGAQALLGDGDWAVGAACKALPTGE
                     PPARPNGKARQ"
     gene            complement(5405..6214)
                     /locus_tag="VGC69_00300"
     CDS             complement(5405..6214)
                     /locus_tag="VGC69_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY0293751.1"
                     /translation="MSAEGSTLPHYQVYAIRYATRDARRASHFIGGDPHDAPMPMDYY
                     LWLVRGAGRQYVIDTGFGADVAKKRGRTLLRTPAAGLALLGVDVKEVGEVVITHLHYD
                     HVGTFDDFPAARFHLQDAEMAYATGRYMCHHPFSHGYEAEEVAGMVRLVYQHRVVFHD
                     GEAELAPGISLHRIGGHTDGLQCVRVHTRRGWVVLASDTCHFYEHFEKQRVFPTMFNV
                     GAALEGYGTLRRLAQTPGHVIPGHDPLVMRKYPAAAPELEGIVVRLDADPL"
     gene            complement(6290..7297)
                     /locus_tag="VGC69_00305"
     CDS             complement(6290..7297)
                     /locus_tag="VGC69_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY0293752.1"
                     /translation="MPSNLQVRFVKPPAGWVDEDCFAFHEAEIPEPAEGEIVVRNVWL
                     SIDPYQRRQMMPGVQYAQPLLLGGVMVGRTMGRVVRSRSSAYREGDWVRGSLGWQQYS
                     LGRVHDLEKIELDGIAPSAYLGVLGSPGITAWVGLREVAKARAGETVVVSAAAGAVGS
                     VAGQLARATGCRTVGIAGGALKCGLATGKFGFDACVDYKDARFGEQLAEAAPGRIDVD
                     FENVGGPIFDQVLQHLNDGARIALCGLVSQYNLVEPYGMKHIGEILNRNAALLGFRVT
                     HYPQCREAALGELKQRLKAGELYFQESVAHGLRSAPAAFVGVLRGTNIGKQVVKIADD
                     A"
     gene            complement(7319..8731)
                     /locus_tag="VGC69_00310"
     CDS             complement(7319..8731)
                     /locus_tag="VGC69_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0293753.1"
                     /translation="MSTTPLKKFDNLSQHLADWVVATGYDDLGQAVVDATTSFVVDHF
                     GVVLAGTQKASSQIIYETCKLWAPVDATSTQVGRSERSSAPFAALVNGTTGHALELDD
                     DHREGTQHPGVAVIPAAFAMAEKLGKSGREFLLAAALGYEIMIRMGEAHLGKSYLRGF
                     HPTGTCGTIGAAVAAGKLLGLNAEQMRSAIGLAASQSAGLLTFRSNGAWNKRLHAGQA
                     AMSGVICAELAARDFRGPSESLLSPEGWLQAFAYNDEFDVHFIVDRLGQKWDLIENSI
                     KVHACCRFAGPIVDATLETVFRENVLPGQVESVTVAMAKYPLTRSLIEPLERKLNPTS
                     VVDAQFSAPFAAAVSIVKRRAFVEEYSETGIRDPEVLAMARKVRWEVDPEAERLWPKR
                     YPCQVTIRLTDGRSVSSRVEWPKGDPENPVTEPELRAKFLTLATPVLGAEGAAASYAA
                     ISGVASADNLAGISAGLIKR"
     gene            complement(8775..10166)
                     /locus_tag="VGC69_00315"
     CDS             complement(8775..10166)
                     /locus_tag="VGC69_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0293754.1"
                     /translation="MSQQIPSPLQARLAEFIVATPYAALPDKFLARLKWSLLDSLGCG
                     LYGSRTEWAGIVARHAVEQGSGPARLWGSPLRVAASSAVLANATMIHSFEIDDVHYGS
                     RSHPGSVTIPVALALIDQGLRVSGQALLCALAVGYEMLARVGACQGVSSFNRGWHPTG
                     TAGVFAATATAARLMGLTQAQCLQALGIAGAMPAGLMAAQYGAMVKRFYAGHAAWVGL
                     TAASLARQGFTGMEDVFDAEYGGYPKALSDAVSLDALTDGLGARYDAGTIGYKFFSCV
                     GTNHTALEAVSELMREHGIAWREVREVRVATSEYQVLHSGWEYKPSTVMAAQMNMQYC
                     IAALLMQGAVFVDQFEERLLADPEVLALAKRVVVNTDAAQDHKDRTAKVEIELAGGRV
                     LAASCSAARGHPDNPPTYEDIERKFLLLAGKIVSPDQCRALSALVGGIESQDDLSGMG
                     ALMAAAAGVPKAS"
     gene            10255..10923
                     /locus_tag="VGC69_00320"
     CDS             10255..10923
                     /locus_tag="VGC69_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0293755.1"
                     /translation="MGFSDQKPRFTSLSETAYNRLRTMIETGELSPRSPLREVHLAQS
                     LEMSRTPVREALRRLEAEGFVDNLPRLGLMVAVLDRHRVIELYEFLEVLECTAARNTA
                     MHATPAEIETLEQYIRQEEKVLEDPAALSALNAKFHDVLYRGARNHYLLKAVHTVRDA
                     IFLLGKSTLATSGRGPRAHVEHLLIVEAIKNRDPEAAYKAMQAHVQSTKRVRLRMVDG
                     EESA"
     gene            complement(10946..11914)
                     /locus_tag="VGC69_00325"
     CDS             complement(10946..11914)
                     /locus_tag="VGC69_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0293756.1"
                     /translation="MHRFIRLSLVLAALIGLMGNAYAKDDYPSKPITLVVPFPPGGAV
                     DIVGRALAQELTLQTGDQVIVENKPGANGVVGTEYVSHAAPDGYTVLLSALGAITIIP
                     HVQKVGYDPMTDLVPVTQAVSLALVWVARQGLPAHSVQELIALDKSGQKLTVGSSGNG
                     SPNHLAIEELNQMAGTHFLHVPYRGEGPALADLMGGHIDLVVTTLVAAAGPLASGHIT
                     ALAAAGAKPPQSMPSLPTIAASGVAGYEAQAWQGVFVPAGTPQPIVDKLNQLMVKVLH
                     SAKVKDYLAKNGTESVGNSAAEFSEFVHNESDRYGKLAKAINLKLD"
     gene            complement(11978..13345)
                     /locus_tag="VGC69_00330"
     CDS             complement(11978..13345)
                     /locus_tag="VGC69_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0293757.1"
                     /translation="MTLHFSTESYTDAAARFASELSLDSVPPEIAGKARACLLYGLGI
                     GLACVDSAYGRIARHALEALDGAAAQRGGATVLATGARLPLSAAAFSNAAMMHGRCQE
                     DTCGSAHLGVAILPCLLALIEADKAPAANLLPALIAGYETAGRIEARLSRASTARGLR
                     ASPLYGTLAAAVAAARAMDLPADRMRAAIANAAAFSGGTLQAIAEGTDEWRYQMGASV
                     RRGLEAAMLARVGAIGAREPLEGALGFAQCYAQTPLDGFDSSPWVLPTVAFKLYPICN
                     RNQTAALLAAHIHGRVAPERIERIRIRINPNVLAGMLERGPFTMLGQTLMSTYFSCAT
                     ALATGTITLRALTDFDAARVAGLIGRMTIDTDAAVPYPSAEAEVQTIEGERLAFSEIR
                     TFEDYSFSREQVNTLLGGLAEETGVPPEAMRMLDRYAYGQTFDSVEPVLQAYATGRAP
                     FVENG"
BASE COUNT         2094 a         4748 c         4514 g         2158 t
ORIGIN      
        1 gcgcccatgt cctcccgccg cagacggtgg aacaggatga tctcgtcgat ccggttgagg
       61 aactcgggcc ggaagtgggc gcgcaccacg cccatcacct cgtcccgcgc ctcctcgaca
      121 tcttcgccgt ccttctgcat ggccaggaac tcggacccga ggttcgaggt catgatgatc
      181 agcacgttgc ggaaatcgac cgtgcggccc tggccgtcgg tcagccgccc gtcgtcgagc
      241 acctgcaaga gcacgttgaa gacgtcgggg tgggccttct cgacttcatc gagcagtatc
      301 acgctgtagg gcttgcggcg tacggcctcg gtgagatagc cgccctcttc gtagcccacg
      361 tatcccggcg gcgcgccgat caggcgggac accgagtgct tctccatgaa ctcgctcatg
      421 tcgatgcgga ccatgtggtc ttccgagtcg aacaagaagt cggccagcgc gcgggtgagc
      481 tcggtcttgc ccacgcccgt ggggcccagg aacaggaagg aacccagcgg gcgcgagggg
      541 tcggacaggc cggcgcgcga gcgccggatg gcgtcggaca ccaggtgcac ggcctcgtcc
      601 tggcccacca cgcggcggtg caaaaagtct tccatcttga gcagcttgtc gcgctcgccc
      661 tgcatcatct tggacacggg gatgccggtg gcgcgcgaga cgacctcggc gatctcttcg
      721 gcgccgacct gggtgcgcaa gaggcgcggc ttgccggcct cggcgccgcc cgcttgctgc
      781 tcggccttgt cggcggcctt caggcgcgct tccagctcgg gcagcttgcc gtattgcagc
      841 tcggcgagct tgtcgaactg gcccttgcgc tgcatctcgg ccatctcggc gcgcacgcgg
      901 tcgatctctt ccttgatgac ctgggtgccc tgcaccgcgg ccttctcggc cttccagatt
      961 tcctcgtagt cgttgtactc gcgctgcagc ttctcgagtt cttcctcgat gacggccagg
     1021 cggcgcttgg aggcgtcgtc ggtttccttc ttgacggcct cgcgctcgat cttgagctgg
     1081 atgatgcggc ggtcgagctt gtccatcact tccggcttgg agtcgatctc catgcggatg
     1141 cgcgcggcgg cctcgtcaat caggtcgatg gccttgtcgg gcaggaagcg gtcggtgatg
     1201 tagcggtgcg agagttcggc cgcggccacg atggccgggt cggtgatctc cacgccgtgg
     1261 tggatttcat agcgttcctg caggccgcgc aggatggcga tggtggactc gacgtcgggc
     1321 tcgtcgacca gcaccttctg gaagcggcgt tcgagcgcgg cgtccttctc gatgtacttg
     1381 cggtattcgt cgagcgtggt cgcgccgatg cagtgcagct cgccgcgcga cagcgcgggc
     1441 ttgagcatgt tgccggcgtc catggcgccc tcggcgcggc cggcgcccac catggtgtgg
     1501 atctcgtcga tgaagacgat gtcctggccc tcgtcctgcg agagctcctt gagcacggcc
     1561 ttcaggcgct cttcgaactc gccgcggaac ttggcgcccg cgagcagcgc ggccaggtcg
     1621 agcgacagca cgcgcttgcc gcgcaggctt tccggcactt cgtcgttgac gatgcgctgc
     1681 gccaggcctt cgacgatggc ggtcttgccc acgccgggtt cgccgatgag cacggggttg
     1741 ttcttggtgc ggcgctgcag gatctggatg gcgcggcgga tctcgtcgtc gcggccgatt
     1801 acggggtcga gcttgcccag gcgggcgcgc tcggtgaggt cgatggtgta cttggccagc
     1861 gcctcgcggt tggactcgcc ctcggcggcc gagacgctct cgccgccgcg cacggcgtcg
     1921 atggcggttt ccagcgcttt cttctgcagg cccgcgtcgc gcaggatgcg gccggcatcg
     1981 cccttgtcgt cggccagggc cagcaggaag agttcgctgg cgatgtaggt atcgccgcgg
     2041 cgggcggctt ccttgtcgac gcgggtgaac acgccctgca attcgcggcc gacctgcacc
     2101 tcgccgccgc cctgcacctg cggcaggttc ttgatggcgg catccagcgc ggcctggacg
     2161 cggttgaccg ccacgccgga acgggccagc aggctggacg cgccgctgtc ggcgtcgccc
     2221 agcagggcgg ccagcacgtg gacgggctcg atatagggat ggtcgttgcg ggcggcgagg
     2281 ctctgggcgt cggccagcgc ttgctggaac ttggtggtaa gtttgtcgaa tcgcatagtg
     2341 gtcggttggc tgatgttgag agccagccgc caggcagatt gcgaggcgcg gctcgatgat
     2401 ctgcatatgg ggtccgggcc acggatttca acacctgtcc atacccgacc cgtgctttat
     2461 gagcctaacc attttcgtct acggcacgct acgcggcggc gaggccaacg acctggccct
     2521 ggccgccgcc cgggcgggcc tgccgccgcc gcaagcgctg ggcccggccc gggtgccggg
     2581 caggctggtg gatttcggcg actggcccgg actcctgccc gacggtcccg gcaccgtgct
     2641 cggcgacctg tacgccgtcg accccagcct ggtcgcgctg atggacgcga tcgaggaata
     2701 caccccggac gccccctgct gcttcgtgcg ccgcccggtc caggcgcgca ccgcggccgg
     2761 tccggtcgac tgccagtact accccatcga tccggccttc cgaggacagg ctcgcgacat
     2821 acccggcgac gactggatcg cctggcggcg gcagcgcagg caccaggcct gatcaggcat
     2881 actgccgctg ttgccgttgc accgccgcac ccaaggaatt tccgcatgac gcttgccaac
     2941 gcttcccccg ccaccgcggc ggcttcgctc accgccctgg aaaccccggc cctggtgctg
     3001 gatgccgagc gcatgcaggc caatgcccgc atgatgctgg atcgcgcccg cagcctgggc
     3061 gtggcgctgc gcccccacgt caagacggcc aaatgcctgg aggtggccct ggccgcctgc
     3121 ggcggcaaac ccgggccgat cacggtctcc accctgaagg aagccgagca gttcttcgcc
     3181 gccggctata ccgacattct ctatgccgtg ggcatcgtcc ccggcaaact ggcccacgtc
     3241 gcccggctgc ggcgcgccgg ctgcgacctg aagatcctgc tcgacaacgc cgccgtggcc
     3301 caggccgtcg cgcaggcccg caagaccgaa gccgtcgacc tgcccacctt gctggagatc
     3361 gacagcgacg gccaccgcag cggcctgcat cccgccgacc cggcgctggc cgccatcgcc
     3421 ggccagctgc gcgcgggcgg cgtcacggtg gccggcgtca tgacccatgc gggcgaatcc
     3481 tacaactgcg gcagcctcgc cgagatcgag gccatggccg aacaggagcg cgccgcctgc
     3541 gtccaggcgg cccagcggct gcgcgccgcg ggccatgcct gccccatcgt cagcgtgggc
     3601 tccacgccca cggcgcgcta cgcgcgcaat ctgcagggcg tgaccgaatt gcgcgcgggc
     3661 gtctacatct tcttcgacct ggtcatggcc ggcctgggcg tctgcacgct cgacgaaatc
     3721 gccctctcgg tgctggtgac cgtgctcggc caccagcccg accgcggctg gatcctgacc
     3781 gacggcggct ggatggccat gagccgcgac cgcggcacgg ccaggcaggc cgaagaccag
     3841 ggctacggcg ccgtctgcga tctggcgggc cgccccatcg ccggcctgaa gatgcgtgac
     3901 gccaatcagg agcacggcat cctggccttc gacgcggcgc aagggtcgcc ggacccgcag
     3961 gcggcattcc ccatcggcac gctgctgcgc atcctgccca accatgcctg cgccaccggc
     4021 gcgcagcacg gcgcctacca ggtggtgcac ggcgactcgc cgcacatcga cgccgtctgg
     4081 ccgcgcttta gcggctggtg acaccaacgc catgaacctg cgcgcgcgat atcggcggct
     4141 gcccggtctg tgggccatgc tgctgctcgc cgcttcggcg tcgagcgcgg cgcagccgcc
     4201 gcccgccccg gccgacccct atgccgaccc cgcgcccgcg ccggcgcgca gcgcgccggc
     4261 ctggcagcgc ctcgagctgg gcgccaaggg ccatcgctat gccttccccg tctatgccag
     4321 ccagcgcctg aatgcgcccg gcgccctgcg caaggtgcag cgcctggtga tcgtgctgca
     4381 cgacgagcgg cgcgaggccg aacgcagcct cgaggccatt accgcgctgt atgccggcca
     4441 ggccgaacat gacgccgaca cgctggtgat cgctcccagg ttcccgggac tggtggacgc
     4501 cggcttcggc agcatgccgg cctggcgccg caccggctgg atggagggcc agcccagcgc
     4561 cgcggcacaa ggcaggccgg ccccggtagg cgccttccag gtcatcgacg acctgttgcg
     4621 cgaactcacc gctcccggcc gcctgccggc gctgcacgcc atcgtggtgg ccggccacgg
     4681 cgccggcgcc cagatggcgc agcgctacgc cgtgctcaat ccgctggacg aaagcctgcg
     4741 cgccaccggc ctggccctga gctatgtggt cgccaatgcc gagtcctacc tgtatctgtc
     4801 gccggaacgg cccaaccgca acggccgcgg ctacacgcgc tatgagcgcg gcatctgccc
     4861 gacctacgac cagtaccgct atgggctgga atcgatgccg gccgccctgg acgcctacca
     4921 gggcaagctc gaccatgccc ggctggccgc ccgctacgcc caacgccgcg tggtctacct
     4981 gatgggcggc gccgacaaca accccgaata ccccggcctg gacaagggct gcggcgccga
     5041 ggcccagggc gccacgccgc tggcccgcgg cctgggatat tggggctatg aaatacgcac
     5101 gccgccgcat ggcgcgccgg ccgcgagggc gggcggcgcg gcaaggccgt cgcaccaggg
     5161 cttcatcgcc atgggcgccg gccacagcga gaccgacctg tatgcctcgg cctgcggcgc
     5221 gcaagccctg ctgggcgatg gcgactgggc ggtgggcgca gcctgcaagg cgctacccac
     5281 cggcgaaccg cctgcccgcc cgaacggcaa ggcgcgccag taggcctggc tttcaatccc
     5341 gccgggcgcc ggtcagcggc aagggcgcgc gcaccgccgg cgcgaagcac gtctcacgct
     5401 tcgcctacaa aggatcggca tccaggcgca ccacgatgcc ctccagttcg ggcgcggcgg
     5461 cggggtactt ccgcatcacc agggggtcgt ggccggggat gacgtggccg ggcgtctgcg
     5521 ccaggcggcg cagcgtgccg tagccctcca gcgcggcccc cacgttgaac atggtgggaa
     5581 agacccgctg cttctcgaag tgctcgtaga aatggcaggt gtcggaagcc agcacgaccc
     5641 agccgcgccg ggtgtgaacc cgcacgcact gcaggccatc ggtgtggccg cctatccggt
     5701 gcaggctgat gccgggcgcc agctcggcct cgccgtcgtg aaacacgacg cggtgctgat
     5761 acaccagccg gaccatgccc gcgacttcct cggcctcgta gccgtggctg aacgggtggt
     5821 ggcacatgta ccggcccgtg gcgtaggcca tctcggcgtc ctgcagatgg aaacgcgccg
     5881 cgggaaagtc gtcgaaggtg cccacgtggt cgtagtgcag atgcgtaatg acgacctcgc
     5941 cgacctcctt cacgtccacc cccagcaacg ccaggcctgc ggccggcgtg cgcagcagcg
     6001 tacggccgcg ttttttggcc acgtcggcgc caaagcccgt gtcgatcacg tactgcctgc
     6061 ctgcgcctcg caccagccac aggtagtagt ccatcggcat gggcgcatcg tgcggatcgc
     6121 cgccgatgaa atggctggcg cgccgggcgt cacgcgtggc gtaccggatg gcgtagacct
     6181 ggtaatgggg cagcgtggag ccttcggcgg acatgggatt tccagagggt gagtggaata
     6241 ggaaatgcgg ggcttcaacg ggccaaacgc cctgattgga acgccgccgc taggcgtcgt
     6301 cggcgatctt cacgacctgc ttgccgatgt tcgtcccgcg cagcacgccg acaaacgccg
     6361 ccggcgcgct gcgcagccca tgcgccacgc tctcctggaa atacagctcc cccgctttga
     6421 ggcgctgttt caactcgccg agcgcggcct cccggcattg cggataatgg gtcacgcgga
     6481 accccagcaa ggctgcattg cggttcagga tctcgccgat gtgcttcatc ccgtagggct
     6541 cgaccaggtt gtactgcgac accaggccgc acaaggcgat gcgcgctcca tcgttgagat
     6601 gctgcagcac ctggtcgaaa atgggcccgc cgacattttc gaaatccacg tcgatcctgc
     6661 cgggcgccgc ctcggccagc tgttcgccga acctggcgtc cttgtaatca acgcacgcat
     6721 cgaagccgaa cttcccggtt gccagcccgc atttcagcgc accgccggca atgccgaccg
     6781 tgcggcagcc ggtcgccctg gccagttggc ccgccacgct gcccaccgcg cccgcggcgg
     6841 ccgacacgac caccgtctcc ccggcccgcg ccttggcgac ctcccgcagg cctacccagg
     6901 cggtgatgcc cgggctgccc agcacgccga ggtaggccga cggggcgatg ccgtcgagct
     6961 cgattttttc caggtcgtgc acgcggccca ggctgtattg ctgccagccc agggagccgc
     7021 gcacccaatc cccttcgcga taggcggacg agcggctgcg gaccacgcgg cccatcgtcc
     7081 ggccgaccat cacgccgccc agcagcagcg gctgggcata ctgcacgccg ggcatcatct
     7141 gccggcgctg ataagggtcg atcgaaagcc atacgttgcg aaccacgatc tcgccctcgg
     7201 ccggctccgg gatctcggct tcatgaaaag cgaaacaatc ctcgtcgacc cagcccgcgg
     7261 gcggcttgac gaagcgaact tgcaggttcg atggcatggc tgtctactcc tcttgcattt
     7321 atcgttttat gagccctgcc gaaatcccgg cgagattgtc ggccgaggcc acgccggaga
     7381 tcgcggcata gctggccgcg gccccttccg cgcccagcac cggcgtggcc agcgtcagga
     7441 acttggcgcg cagttccggc tcggtcaccg ggttttccgg atcgcccttg ggccactcca
     7501 cccgcgagga aacggaacgc ccgtcggtca agcggatggt cacctggcac ggatagcgct
     7561 tgggccacag gcgctcggcc tcgggatcta cctcccagcg caccttgcgg gccatggcca
     7621 gcacctccgg atcacgaatg ccggtctcgc tgtattcttc cacgaaggcg cgccgcttga
     7681 cgatggacac ggccgccgcg aagggcgcgc tgaactgcgc gtccaccacc gaggtcgggt
     7741 tgagcttgcg ctccagcggc tcgatcagcg agcgggtcaa cggatacttg gccatcgcca
     7801 ccgtcacgga ctccacctgc ccgggcagca cattctcgcg gaacaccgtc tccagcgtcg
     7861 cgtccacgat ggggccggca aaccggcagc aggcatgcac cttgatgctg ttctcgatca
     7921 ggtcccactt ctggcccagg cggtcgacga tgaaatggac gtcgaactcg tcgttgtagg
     7981 cgaaggcctg cagccagccc tcggggctga gcaggctttc gctcgggccg cggaaatcgc
     8041 gcgccgccag ctcggcgcag atgacgccgg acatggccgc ctggcccgca tgcagccgct
     8101 tgttccacgc gccgttggag cggaaggtca gcaggccggc cgactggctg gccgccaggc
     8161 cgatggcgct gcgcatctgc tcggcgttca ggcccagcag cttgcccgcg gcaacggcgg
     8221 cgccgatcgt gccgcaggtg ccggtgggat ggaaaccccg caggtacgat ttgcccaggt
     8281 gcgcctcgcc catgcggatc atgatctcgt aacccagcgc cgcggccagc aggaattcgc
     8341 ggccgctctt gcccagcttc tcggccatgg cgaatgccgc cgggatcacg gcgacgccgg
     8401 ggtgctgcgt cccttcgcga tggtcgtcgt ccagttccag cgcgtggccg gtggtgccgt
     8461 tgaccagggc ggcgaaaggc gccgagctgc gttcgctgcg accgacctgg gtcgacgtcg
     8521 cgtcgacggg cgcccacagc ttgcacgttt cgtagatgat ctgggacgag gccttctgcg
     8581 tgcccgccag cacgacgccg aagtggtcca cgacgaagct cgtggtggcg tcgaccacgg
     8641 cttgcccaag atcgtcgtat cccgtggcca cgacccaatc ggccaggtgc tgggacaggt
     8701 tgtcgaattt cttcaaaggt gtcgtgctca tcgttgtcct tgcggtaaaa gtgggggtgt
     8761 cgaaaagagg gttgtcatga agccttcgga acgccggcag ccgccgccat cagggcgccc
     8821 atgccggata ggtcgtcctg cgactcgatc ccaccgacca gcgcgctgag cgcacggcac
     8881 tgatcggggc tgacgatctt gcccgccagc agcaggaact tgcgctcgat gtcttcatag
     8941 gtgggcgggt tgtccgggtg gccgcgcgcc gcgctgcacg aagccgccag gacgcggccg
     9001 ccggccagct cgatctccac cttggcggtg cggtccttgt ggtcctgggc ggcgtcggta
     9061 ttcaccacga cgcgcttggc caacgccagc acctcgggat cggccagcag gcgctcctcg
     9121 aactgatcga cgaacacggc gccctgcatc agcagcgccg cgatgcagta ctgcatgttc
     9181 atctgggccg ccatcacggt ggacggcttg tattcccagc cggagtgcag cacctggtat
     9241 tcggaggtgg ccacgcgcac ctcgcgcacc tcgcgccagg ctatgccgtg ctcgcgcatc
     9301 agctccgaca cggcttccag ggccgtgtgg ttggtgccca cgcaggagaa aaacttgtag
     9361 ccgatggtgc ccgcgtcgta gcgcgcgccc aggccatccg tcagcgcatc cagcgacacc
     9421 gcgtcggaca gcgccttggg atagccgcca tattcggcgt cgaacacgtc ttccatgccg
     9481 gtgaagccct gcctggccag cgaggcggcc gtcaggccca cccatgcggc atggccggca
     9541 tagaagcgct tgaccatggc gccgtactgg gccgccatca ggccggccgg catggcgccg
     9601 gcgatgccga gcgcctgcag gcattgcgcc tgggtcaggc ccatcaaccg ggccgcggtg
     9661 gcggtggcgg cgaaaacacc cgcggtgccg gtgggatgcc agccgcgatt gaacgacgaa
     9721 accccttggc aggcgccgac ccgcgccagc atctcatagc cgaccgccag ggcgcacagc
     9781 aacgcctggc ccgatacccg caggccctgg tcgatcagcg ccaaggccac cgggatcgtc
     9841 acgctgcccg gatgggaacg gctgccatag tgcacgtcgt cgatttcgaa gctgtggatc
     9901 atggtggcat tggccagcac cgcggaactc gcggcgaccc gcagcggcga cccccacagc
     9961 cgggccgggc cgctgccctg ctccaccgca tggcgcgcga cgatccccgc ccactcggtg
    10021 cggctgccgt acaagccgca gcccagggaa tcgagcagcg accacttcag cctcgccagg
    10081 aacttgtccg gcagcgccgc gtagggggtg gcgacgatga actcggccag ccgcgcctgc
    10141 agcggcgacg ggatctgctg actcatggtt ttctccgatg atataatttg tatatcaatg
    10201 aatacataga tggggaaaaa gccccgcaga cgacatcggg ggtcatgctt ctacatgggc
    10261 ttttcggacc aaaaaccgcg tttcacctcg ctcagcgaga cggcctacaa ccgcctcaga
    10321 accatgatcg aaaccggtga gctgagcccg cgcagcccct tgcgggaagt tcacctggcg
    10381 cagtcgctcg aaatgagccg cacccccgtg cgcgaagcac tgcgccggct cgaggcggag
    10441 ggcttcgtcg acaacctgcc ccgcctcggc ctgatggtcg ccgtgctgga ccgccaccgc
    10501 gtcatcgagc tgtacgagtt cctggaggtg ctggaatgca ccgccgcccg gaacacggcc
    10561 atgcacgcca cgcccgccga aatcgaaacg ctcgagcaat acatccggca ggaagaaaaa
    10621 gtgctggaag accccgccgc cctgtcggcc ctcaacgcca aattccacga cgtcctctac
    10681 cggggcgccc gcaaccacta tctgctcaag gccgtgcaca cggtgcgcga cgcgatcttc
    10741 ctgctcggca agtccaccct ggccacgtcc ggccggggcc cgcgcgcgca tgtcgagcac
    10801 ctgctcatcg tcgaggccat caagaaccgc gacccggaag ccgcctacaa ggccatgcag
    10861 gcgcacgtgc agtccaccaa gcgcgtgcgc ctgcgcatgg tcgacggcga agaaagcgct
    10921 tgagccggtc atcgtcgtcg ccatgtcaat ccagcttcag gttgatggcc ttggccagct
    10981 tcccgtagcg gtcggattcg ttgtgcacga actcgctgaa ttcggccgct gaattgccca
    11041 cggactcggt gccgttcttg gccagatagt ccttcacctt ggctgagtgc agcaccttga
    11101 ccatcaactg gttgagtttg tcgacgatgg gctggggcgt gcccgcggga acgaatacgc
    11161 cctgccatgc ctgcgcctcg tagccggcca cgcccgacgc cgcgatcgtg ggcaggctgg
    11221 gcatggattg aggcggcttg gctcccgcgg cggccaaggc ggtgatgtgc ccgctggcca
    11281 gcggcccggc agccgccacc agcgtggtga cgaccaggtc gatgtgcccg cccatcaagt
    11341 ccgccagcgc cggaccctcg ccgcgatacg gcacgtgcag gaaatgcgtg cccgccatct
    11401 ggttgagctc ttcgatcgcc aagtggttgg ggctgccgtt gcccgacgag ccgaccgtca
    11461 acttctgccc ggacttgtcc agggcgatga gttcttgcac gctgtgcgcc ggcaagccct
    11521 gcctggcaac ccagaccagc gccagcgaaa cggcctgcgt caccgggacc aggtccgtca
    11581 tggggtcgta gccgaccttc tgcacgtgcg ggatgatcgt gatcgcgccc agggcgctca
    11641 gcagcaccgt atagccgtcc ggcgccgcgt gcgacacgta ctccgtgccc accaccccgt
    11701 tggcgccggg cttgttctcg acgatgacct ggtcgccggt ctgcaaggtc agttcctgcg
    11761 ccagcgcccg gcccacgatg tcaacggcgc cgcccggcgg gaacggcacg accagggtaa
    11821 tgggcttgga ggggtagtcg tccttggcgt aggcattgcc catcagcccg atgagggctg
    11881 ccaagaccaa tgaaagccga atgaagcggt gcatgttgtc tcctgtttat ggtgttgtgc
    11941 gcggttcgtg ctctgcggca cggtccgtgt gttgtcgtca gccgttctcc acgaacggcg
    12001 cccggcccgt ggcgtaggcc tgcagcaccg gctcgaccga atcgaaggtc tgtccgtagg
    12061 cgtatcgatc cagcatccgc atggcctcgg gcggcacgcc ggtctcctcg gccaggccgc
    12121 ccagcagggt gttgacctgc tcgcgggaga agctgtagtc ctcgaaggtg cggatctcgc
    12181 tgaacgccag gcgctcgccc tcgatggtct gcacctcggc ctcggccgac ggatagggaa
    12241 ccgcggcatc ggtgtcgatg gtcatgcggc cgatcaggcc ggccacccgt gcggcgtcga
    12301 aatccgtcag cgcgcgcagc gtgatcgtgc ccgtcgccag cgccgtcgcg cagctgaagt
    12361 aggtgctcat caaggtctgc cccagcatcg tgaacggccc gcgctccagc atgccggcca
    12421 gcacattggg gttgatgcgt atccgtatgc gctcgatgcg ttccggggcg acccggccgt
    12481 ggatgtgcgc cgccagcagg gcggcggtct ggttgcggtt gcagatgggg tagagcttga
    12541 acgccaccgt gggcagcacc cagggactcg aatcgaagcc gtccaatggc gtttgcgcgt
    12601 agcactgggc aaagcccagg gcgccttcaa gcggctcgcg cgcgccgatc gcgccgacgc
    12661 gggcgagcat ggccgcttcc aggccgcggc gcaccgacgc gcccatctgg tagcgccatt
    12721 cgtcggtccc ctcggcaatc gcctgcaggg tgccgcccga gaacgcggcc gcattggcga
    12781 tggccgcccg catgcggtcc gcgggcaggt ccatggcgcg cgccgccgcc acggccgcgg
    12841 ccagggtgcc gtagagcggg gaggcgcgca aaccccgggc cgtcgaggcg cggctcaagc
    12901 gcgcctcgat gcggcccgcg gtttcgtagc ccgcgatgag cgccggcagc aggttcgcag
    12961 ccggcgcttt gtccgcctcg atcagggcca gcaggcaagg cagtatggcc acccccagat
    13021 gcgccgaacc gcaggtgtct tcctggcagc gcccgtgcat catcgccgcg ttgctgaagg
    13081 ccgcggccga cagcggcaaa cgcgcgcccg tcgcgagcac cgtcgccccg ccccgctgcg
    13141 ccgccgcgcc gtcgagagcc tccagggcgt gccgggcgat ccggccgtaa gcgctgtcga
    13201 cgcaggccag gccaatgccc aggccgtaca gcaggcaggc gcgcgccttc ccggcaatct
    13261 cgggcggcac gctgtccagg gacagctcgc tcgcaaagcg ggcggccgca tcggtatagc
    13321 tttcggtgga aaagtgcagg gtcacggctg atctctattt ttgtatacat agaaatacat
    13381 ttgtgcatag tacacaccgc cccagggatg ggcaagactt ttgaaaaaag aaaaagccgg
    13441 ctgaaaagcc ggcttggacg ctacccgcgg agcaggagca agggaacggg aaacggcctc
    13501 agcgcttcgc gccc
//
LOCUS       DASWPO010000009         6395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1697809, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000009 DASWPO010000000
VERSION     DASWPO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697809"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3088)
                     /locus_tag="VGC69_00335"
     CDS             complement(<1..3088)
                     /locus_tag="VGC69_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0293758.1"
                     /translation="MKWQFWIDRGGTFTDIVARRPDGSMATAKMLSENPEQYRDAAVA
                     GIRKLLGIAPGQPVPAERVDCVKMGTTVATNALLERKGEPTLLVTTRGFRDALRIAYQ
                     NRPRLFDRNVVLPEMLYTSVVEADERLDAAGGVVRPLDETALRRDLQAAYDQGLRAVA
                     IVFMHAWTMSGHEQRAAELARAIGYAQVSVSHEVSPLIKYVSRGDTTVVDAYLSPILK
                     RYVDQVAGELPGIRLMFMQSSGGLTDAHRFRGKDAILSGPAGGIVGMVRTSELAGFDH
                     VIGFDMGGTSTDVSHYAGEFEREFETRVAGVRMRAPMMSIHTVAAGGGSVLHFDGARL
                     RVGPDSAGANPGPACYRRGGPLAVTDCNVLLGKIQPDFFPHVFGPAADQPLDREAVAR
                     RFAEMAAQVRQDTGREMTSEQLAEGFLEIAVGNMAEAIKRISVQRGHDVTAYALTVFG
                     GAGGQHACLVADALGMSTVYAHPLAGVLSAYGMGLADQTEMRQRTIEKVLSPELVAEL
                     SAGLDELAAQAEGELLRQQVPADQIRVQRRLHLKYRGTDTALEVPYDTPQAARAAFEA
                     AYRQRYSFLMPGRQLVVDTISVEATGGGETTAEALVVRTRAGALMPRRTVRMFSGGDW
                     HDTPLYVREDTVPGDAMSGPAIISEPNQTTVVEPGWEARVTELDHLVLKRVVARPARR
                     AIGTEADPVMLEVFNNLFMSIAEQMGYRLQNTAYSVTIKERLDFSCAIFDAEGNLIAN
                     APHMPVHLGSMSESIKTVMRENAGRMQPGDAYVVNDPYNGGTHLPDLTVITPVFDRAG
                     EEILFYVGSRGHHSDIGGTTPGSMPPDSRTVDEEGVLFTNFQLVRDGEFREAAVRDVL
                     ASGKWPARNPDQNIADMQAQIAANEKGVHELLKMCEHFGLDVVRAYMNHVQDNAEEAV
                     RRVISVLKNGRYEYPLDNGAVIKVAVRVDAASRSAEVDFTGTSAQLDNNFNAPSSISV
                     AAVLYVFRTLVDDEIPMNAGCLKPISIIVPEGSMLRPLPPASVVAGNVETSMCVVNA"
     gene            complement(3279..3986)
                     /locus_tag="VGC69_00340"
     CDS             complement(3279..3986)
                     /locus_tag="VGC69_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0293759.1"
                     /translation="MLKVEGLHVSYGGVQAVQDVSLEVHPGEIAALLGANGAGKSSTL
                     MAIIGSVKPKGGRVIFQGRDITGTPPEKLVTQGIAMIPEGARVFARQPVEQNLRLGAY
                     TVRDESVYRQRLAHVYRLFPRLEERREQLAGTMSGGERQMLAIGRALMSGPRLLLIDE
                     PSLGLSPLLVEQVFDALAELNRKGGLTVLLVEQNMAQALEVARRAYVMQSGRIALSGD
                     ADELAASDEVRKAYLGM"
     gene            complement(3991..4713)
                     /locus_tag="VGC69_00345"
     CDS             complement(3991..4713)
                     /locus_tag="VGC69_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0293760.1"
                     /translation="MALLDVKNLTKRFGGLVANKDISLSVEAGEIVAIIGPNGAGKST
                     LFNGLVGHHEPTSGTVEFNGLSMVGKKPEQVAAMGLVRTYQIPRNFGQMTVLENAMVG
                     AMLRHPRLEDARREAARVLELVDLAGRAGIKAAELNVAGQKRVELARALATEPRMLLL
                     DEVAGGLNPTEAIALAEILRGIHAAGVTLVIVEHVLEVVMRLAQRVLVLNFGQMIAEG
                     PPAEIVRNPTVIEAYLGRKHRV"
     gene            complement(4694..5680)
                     /locus_tag="VGC69_00350"
     CDS             complement(4694..5680)
                     /locus_tag="VGC69_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0293761.1"
                     /translation="MTTQTLARGVKDRHWPVLAAAVIAILMALVPYVLKDAFTLQVLL
                     LAIMFGALGACWNLVGGFLGRISFGHGVFIGTGAYTTLLLLHYWKITPLIGIPLGGVI
                     SALLAWIVGGPTLRLSGHYFAMATIALLQIGLLMLINWDWAGGATGLEAPIGDAAWNL
                     LFRTKVPYYRIAVLLAFLTFCATYFLVHSRVGFYWRAINGDEAAARSLGVPADRYKML
                     AFVISAGMTGVWGGFFAMYVGFIDPDSMFNLTTSVQMVLVSILGGVGTLVGPWLGAAV
                     LLPLSESTRVMLGSSGLGLDLLVFGLAILLVTMFLPGGLVTLWRRRRGTAGR"
     gene            complement(5682..>6395)
                     /locus_tag="VGC69_00355"
     CDS             complement(5682..>6395)
                     /locus_tag="VGC69_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0293762.1"
                     /translation="AYWMFTLGHVDPTLSAPLVAILLGFVGFLTYALIIKRLQKGPPM
                     AVILATFGLGLVLRQLAFIAFTPDFRNLPDTLLSGSLSLGPVSLGRPSVVTGVIALVL
                     VAVLFVLVYRTRWGHALQAVAEDRQVAALVGISPDRVNAQVWMLGSASVGLAGALLTT
                     FFYVFPSVGTVFGLLAFVAVCMGGFGSLPGAVIAGILIGLIEAMTGYFVAPALKTASV
                     FILFILVLWYRPRGLFGRW"
BASE COUNT         1115 a         2240 c         2108 g          932 t
ORIGIN      
        1 gggcgttgac cacgcacatc gaggtctcga cattgccggc caccaccgag gccggcggca
       61 gcgggcgcag catcgaccct tcgggcacga tgatgctgat gggtttgagg caccccgcgt
      121 tcatggggat ctcgtcgtcc accagcgtgc ggaacacata cagcacggcc gccaccgaaa
      181 tcgagctggg cgcgttgaag ttgttgtcca gctgcgccga ggtgccggtg aagtccacct
      241 cggccgagcg cgaggcggca tcgacccgca cggccacctt gatcaccgcg ccgttgtcca
      301 gcgggtactc gtaccggccg ttcttcagca ccgagatcac gcggcgcacc gcctcctcgg
      361 cattgtcctg cacgtggttc atataggcgc gcaccacgtc caggccgaag tgctcgcaca
      421 tcttcagcag ctcgtgcacg cccttttcgt tggcggcgat ctgcgcctgc atgtcggcga
      481 tgttctggtc gggattgcgg gccggccact tgccggaagc cagcacgtcg cgcaccgcgg
      541 cctcgcggaa ctcgccgtcg cgcaccaatt ggaagttggt gaaaagcacg ccctcctcgt
      601 ccaccgtgcg cgagtcgggc ggcatggagc ccggcgtggt gccgccgatg tccgaatggt
      661 gcccgcgtga gcccacgtag aacaggattt cttcgccggc gcggtcgaac acgggcgtga
      721 tcaccgtcag gtcgggcaag tgcgtgccgc cgttgtaggg gtcgttgacc acataggcgt
      781 cgcccggctg catgcggccg gcgttttcgc gcatcacggt cttgatcgac tcgctcatcg
      841 aacccaggtg caccggcatg tgcggcgcat tggcgatgag gttgccctcg gcatcgaaga
      901 tcgcgcagga gaagtccagg cgctccttga tggtgaccga atacgccgtg ttctgcaggc
      961 ggtagcccat ctgctcggcg atcgacatga agaggttgtt gaacacctcc agcatcaccg
     1021 ggtcggcctc ggtgccgatg gcccgccgcg ccggccgggc caccacgcgc ttgagcacca
     1081 ggtggtccag ttccgtgacg cgggcttccc agccgggttc gaccacggtg gtctggttgg
     1141 gctcggaaat aatggccgga ccggacatcg cgtcgccggg caccgtgtcc tcgcgcacgt
     1201 acaggggcgt gtcgtgccag tcgccgccgc tgaacatgcg caccgtgcgg cgcggcatca
     1261 gcgcgcctgc gcgggtacgc acgacaagcg cctcggccgt cgtctcgccg ccgccggtgg
     1321 cttcgaccga gatcgtgtcc accaccagtt ggcggccggg catcaggaag gagtaacgct
     1381 gccggtaggc cgcctcgaag gccgcgcgcg ccgcctgcgg cgtgtcgtaa ggcacttcca
     1441 gcgccgtgtc ggtgccgcgg tacttgaggt gcaggcggcg ctgcacgcgg atctggtcgg
     1501 caggcacctg ctggcgcagc agctcgccct cggcctgcgc ggccagttcg tccagacccg
     1561 ccgagagttc ggccacgagt tcgggcgaca gcaccttctc tatcgtcctc tgccgcatct
     1621 cggtctggtc ggccaggccc atgccgtagg ccgacagcac gccggccagc gggtgggcat
     1681 acaccgtgct catgcccagg gcgtcggcca ccaggcaggc atgctgcccg cccgcgccgc
     1741 cgaacaccgt cagcgcatag gcggtgacgt catggccgcg ctgcaccgag atgcgcttga
     1801 tggcctcggc catattgccc accgcgattt ccaggaagcc ctcggccagc tgctcggacg
     1861 tcatctcgcg gccggtgtcc tggcgcactt gcgcggccat ctccgcaaag cgccgcgcca
     1921 cggcctcgcg gtccagcggc tggtcggcgg cggggccgaa cacatggggg aagaagtcgg
     1981 gctggatctt gcccagcagc acgttgcagt cggtcacggc cagcggaccg ccgcgccgat
     2041 agcaggccgg gccggggttg gcgccggccg aatccggccc cacgcgcagg cgcgcgccat
     2101 cgaaatgcag gacggacccg ccgcccgccg ccacggtgtg gatgctcatc atgggcgcgc
     2161 gcatgcgcac gccggccacg cgcgtctcga actcgcgctc gaactcgccc gcgtagtgcg
     2221 acacgtcggt ggaggtgccg cccatgtcga agccgatcac gtggtcgaag cccgccagct
     2281 cgctggtgcg caccatgccc acgatgccgc ccgccggacc ggacaggatc gcgtccttgc
     2341 cgcgaaagcg atgggcgtcg gtcaagccgc cggacgactg catgaacatc agtcggatgc
     2401 cgggcaactc gcccgccacc tggtccacat agcgcttgag tatgggcgac aggtaggcgt
     2461 cgaccaccgt ggtgtcgccg cgcgacacgt acttgatgag cggactgacc tcgtgcgaga
     2521 cggacacctg cgcgtagccg atcgcgcgcg ccagttcggc ggcgcgctgt tcatggccgg
     2581 acatggtcca ggcatgcata aagacgatgg ccaccgcgcg caggccctgg tcgtaggcgg
     2641 cctgcagatc gcggcgcaag gcggtttcgt ccaacggcct caccacgccg cccgcggcat
     2701 ccaggcgctc gtcggcctcg accaccgagg tgtagagcat ctcgggcagc acgacattgc
     2761 ggtcgaacag ccgcggccgg ttctggtagg cgatgcgcag cgcatcgcgg aaaccgcgcg
     2821 tggtcaccag caaggtgggc tcgcccttgc gttccagcaa ggcgttggtg gccacggtgg
     2881 tgcccatctt cacgcagtcc acccgctcgg ccggcacagg ctgcccgggc gcgatgccca
     2941 gcagcttgcg gatgcccgcc accgccgcgt cgcggtactg ctcggggttc tccgacagca
     3001 tcttggccgt ggccatgctg ccatcggggc gccgcgccac gatgtcggtg aacgtgcccc
     3061 cacgatcaat ccagaattgc catttcataa aacacatcca aaaaggttgt aagaaactcg
     3121 agacgatgca gcattgccgc cgtccctggg ttgtagcgac cagtaggagg aagggaagcc
     3181 cgaagggccg agggcgcgtc gccttgcgcc cgagccgcgc cgacgtggtc gctacagccc
     3241 agggacggcg gcaatgcgcc tttagaactc aataaactct acatacccaa ataagccttg
     3301 cgcacctcat cagaagccgc caactcatca gcatccccgg acaaggcaat ccgcccgctc
     3361 tgcatcacat aggcgcgcct ggccacttcc agcgcctgcg ccatgttctg ctcgaccagc
     3421 agcacggtca ggccgccttt gcggttgagc tcggccagcg cgtcgaacac ctgctccacc
     3481 agcaaaggcg acaggcccag gctgggctcg tcgatcagca gcaagcgtgg cccgctcatc
     3541 agcgcccgcc cgatcgccag catctgccgc tcgccgcccg acatggtgcc ggccagttgc
     3601 tcgcggcgtt cctccaggcg cgggaacaaa cggtatacgt gggccaaccg ctggcggtag
     3661 acggactcgt cgcgcaccgt ataggcgccc agccgcaggt tctgctcgac cggctggcgg
     3721 gcaaacacgc gcgcgccttc cgggatcatg gcaatgccct gcgtgacgag cttctcgggc
     3781 ggcgtgccgg taatgtcgcg gccctggaaa atgacccgcc cgcccttggg cttgaccgag
     3841 ccgatgatgg ccatcagcgt gctggacttg cccgcgccat tggcgcccag caaggccgcg
     3901 atctcgcccg gatgcacctc cagcgacacg tcctgcacgg cctgcacgcc gccgtacgac
     3961 acatgcagcc cttcgacttt gagcatgggc tcagacacga tgcttcctcc ccagataggc
     4021 ctcgatcacg gtcggattgc gcacgatctc ggccggcggg ccttcggcga tcatctgccc
     4081 gaagttcagc accagcacgc gctgggccag gcgcatcacc acctcgagca cgtgctcgac
     4141 gatgaccagc gtcacgcccg ccgcgtggat gccgcgcagg atctcggcca gcgcgatggc
     4201 ctcggtggga ttcaagcccc ccgccacttc gtcgagcagc agcatgcgcg gctcggtggc
     4261 cagcgcgcgg gccagctcca cccgcttctg cccggccacg ttgagctcgg cggccttgat
     4321 gccggcccgg ccggccaggt ccaccagctc gagcacgcgg gccgcctcgc ggcgcgcgtc
     4381 ttccagcctg gggtggcgca gcatggcgcc gaccatggcg ttctccagca ccgtcatctg
     4441 gccgaagttg cgcgggatct ggtaggtgcg caccaggccc atggccgcca cctgctcggg
     4501 tttcttgccc accatcgaca ggccgttgaa ttccaccgtg cccgaggtgg gctcgtggtg
     4561 gccgaccagc ccgttgaaca gcgtgctctt gcccgccccg ttgggcccga tgatggcgac
     4621 gatctcgccg gcctcgaccg acagcgagat atccttgttg gccaccaggc cgccgaagcg
     4681 cttggtgagg ttcttaacgt ccagcagtgc cacggcggcg cctccacaag gtaaccagcc
     4741 cgccgggcag gaacatggta acgagcaaaa tggccaggcc gaataccagc aggtccaggc
     4801 ccagccccga gctgcccagc atcacgcggg tgctctcgga gagcggcagc agcacggcgg
     4861 cccccagcca cggcccgacc agcgtcccca ccccgcccag gatggacacc agcaccatct
     4921 gcaccgacgt ggtcaggttg aacatggagt ccgggtcgat gaagcccacg tacatggcga
     4981 agaagccgcc ccacacgccc gtcatgccgg ccgagatcac gaaggccagc atcttgtagc
     5041 ggtcggccgg cacgcccagg ctgcgggcgg ccgcctcgtc gccgttgatg gcgcgccagt
     5101 agaagccgac gcgcgaatgc accaggaagt acgtggcgca gaaggtcagg aaggccagca
     5161 gcacggcgat gcggtagtac ggcaccttgg tgcggaacag caaattccag gccgcgtcgc
     5221 cgatgggcgc ttccagcccg gtggcgccgc ccgcccagtc ccagttgatg agcatcagca
     5281 ggccgatctg cagcaaggcg atggtggcca tggcgaaata atggcccgac aggcgcaagg
     5341 tcggtccgcc cacgatccag gccagcaagg ccgagatcac gccgcccagc gggatgccga
     5401 taagcggcgt gatcttccag tagtgcagca gcagcaaggt ggtgtaggcc cccgtgccga
     5461 tgaacacgcc gtggccgaag gaaatgcggc ccagaaagcc gcccaccagg ttccagcacg
     5521 cgcccagcgc gccgaacatg atggccagga gcagcacctg cagggtgaag gcatccttga
     5581 gcacataggg caccaaggcc atcaatatcg cgatgacggc ggcggccagc accggccagt
     5641 gcctgtcctt cacgccgcga gcgagggttt gagtcgtcat gtcaccaccg cccgaacagg
     5701 ccgcgcggcc gataccagag caccaggatg aagaggatga acacgctggc cgtcttgagc
     5761 gccggggcca cgaaatagcc cgtcatggcc tcgatcaggc cgatcaggat gccggcgatg
     5821 acggcgcccg gcagcgaacc gaatccgccc atgcacaccg ccacgaaggc cagcagcccg
     5881 aacaccgtgc ccaccgacgg gaacacgtag aaaaaagtgg tgagcaaggc gccggccagg
     5941 cccaccgagg cgctgcccag catccacacc tgggcgttga cccggtccgg cgagatgccc
     6001 accagcgccg ccacctggcg atcttccgcc acggcctgca gggcgtgtcc ccagcgcgtg
     6061 cggtagacca gcacgaagag cacggccacc agcaccaggg cgatcacgcc cgtgacgacc
     6121 gaaggccggc ccagcgagac cgggcccagc gacaggctgc ccgagagcag ggtgtcgggc
     6181 aggttgcgga agtccggcgt gaaggcgatg aacgccagct ggcgcagcac cagacccagg
     6241 ccgaaagtgg ccaggatgac cgccatgggc ggccccttct gcaggcgctt gatgatcagc
     6301 gcataggtga gaaaccccac gaagcccagc aaaatggcga ccaggggcgc cgacagggtg
     6361 gggtcgacgt ggcccagggt gaacatccaa taggc
//
LOCUS       DASWPO010000010         6030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1519936, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000010 DASWPO010000000
VERSION     DASWPO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519936"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGC69_00360"
     CDS             complement(<1..713)
                     /locus_tag="VGC69_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0293763.1"
                     /translation="MSASGTQPAPKRASPLQIDIARRIARQVVEGELAPGSHLTEEAL
                     AAEFKVSRSPVRAALQLLAGRGLIQYRTNSGYFVLERQEGMEQPDFEESGMGIDGLYQ
                     QLVDARARRLLPDTVLEKDLLLAYPVSRRLLAKALMRMLADGLVEKRPGHGWAFLPSL
                     ETPEALAESYRFRMLVECGGLLEPTFSADSVQLARCRQAYEQWIASDFTDLSPGKFFT
                     LNASFHEMLARFSGNRFIL"
     gene            complement(779..1528)
                     /gene="gspN"
                     /locus_tag="VGC69_00365"
     CDS             complement(779..1528)
                     /gene="gspN"
                     /locus_tag="VGC69_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY0293764.1"
                     /translation="MRSSRIAVSLLAIVVALASAAVVLPARWLLSWVPASGPLAVVDA
                     DGTIWHGQAWIAIGYPETRRMLTDPVQWQWRGWTMALRHPWLRGPLVLAPGWDGMRLS
                     AQQVRLPAAALTALGAPFNTMGPGGFLEVSWNALSTHQPQAAGPLLDLLWRDASSAVT
                     PLASLGDFRLRVASTGQALTLDLSTEHGPLDLTGHGAWDGRALRFQGQAKTTGDATAE
                     QAAGLNGLLNLIGPRRGGAHVFGFPTLAAVP"
     gene            complement(1562..2155)
                     /gene="gspM"
                     /locus_tag="VGC69_00370"
     CDS             complement(1562..2155)
                     /gene="gspM"
                     /locus_tag="VGC69_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEY0293765.1"
                     /translation="MNLSETLLPAWRGLRARYATLHAQVTAWLQQLTLRERQLVIGLA
                     VLVPAVLVWLLLVQPALDTVGQLQAELPKLRSQAAVVADLAARSGGLQRHAARMANAA
                     PTIADLQSSLQQSGLPAAQWSLQATSADAAKPQWTLKLDQAQALVLLRWLDDVSRDLR
                     LTVASVELDRALDADKRRLPGRVDGVVTLEPTAPRAK"
     gene            complement(2152..3147)
                     /gene="gspL"
                     /locus_tag="VGC69_00375"
     CDS             complement(2152..3147)
                     /gene="gspL"
                     /locus_tag="VGC69_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEY0293766.1"
                     /translation="MKNLLRIALPALADLTASLPLAYARFDRSGRCVQRGSLPAAALA
                     NAFPRLPIQAVLHAHDAIVAAVQVPAVPKSRFPDAVRSALEALVLGELDSLAVGHGLR
                     SRDGEVTVAWADKEALRRAWLLLSQAGLEIRALIPVQALGGGQAPDAPLLEPTDPRWL
                     ADTPSWSLALPQLAPRQVSHWRPVWRWAVAAAVLWVAGLNLYAMQLRGEATRLRDTMV
                     TQVRDAFPGIPVVLDPVRQAQQGYDALATGSSSGTPGDFIALARAGAQALPFAADQVD
                     KLVYRDQALDLQLADQGGSDHLGDMPAIMQKASASGIRVEHGDNSATWRIVPVQP"
     gene            complement(3144..4178)
                     /gene="gspK"
                     /locus_tag="VGC69_00380"
     CDS             complement(3144..4178)
                     /gene="gspK"
                     /locus_tag="VGC69_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEY0293767.1"
                     /translation="MVTFQPTDPHRQRGAAVIAALIIVAIVAALCSSLFLRQTATVRQ
                     VENEEARVQARWLLTGGIDWARLILREHSSRESSTQGDQIWAVPVQDTRITQPGDDRV
                     ALFSGMLEDEQGKFNLYNLASNGVIAADQLALLQRLMANLNLPASLAQQMAALIAQSQ
                     PRGVLESAGTPAAAGVTGSVASTPDQTNAAAGLAGASAQANTAASPAAAPMPRNIDDV
                     AGFLGADEATRLVLARAMTVLPVNTVINVNTAPPEVIAALVPGLSLAQASNLTGDRDR
                     GHWFVDTGDFANRIASLVPEVPAPQVTVTSAWFMATGTVAYERARLTLRALLSSVGGG
                     TSLLWTQEIS"
     gene            complement(4172..4774)
                     /locus_tag="VGC69_00385"
     CDS             complement(4172..4774)
                     /locus_tag="VGC69_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0293768.1"
                     /translation="MTAGHALPRQQGFTLIEVLVAIALMALVSLLAWRGLDRVAQAHT
                     RIDEQAQDNDMIVSVLGQLERDVQQAYVGVPRPLGADGALPGGIQMSKGAMPSLDIVR
                     AAPDQPGRWVHVLWQLRADGLWRYVGASGDRYPLPAPSSGALIMPNATTFHLRAWVPG
                     RGWADLPASLAQHAAGLDIMLARQRQGAVQPFDRIVLLPW"
     gene            complement(4771..5166)
                     /gene="gspI"
                     /locus_tag="VGC69_00390"
     CDS             complement(4771..5166)
                     /gene="gspI"
                     /locus_tag="VGC69_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEY0293769.1"
                     /translation="MRSTEAPRARDRQRGFTLIEVLVALAIIAIAMGAAIRATGVMID
                     NNLALLDKSLALLAAENVLAQLRLEQTVPQPGRDTVPCPEGGRNLRCERVFTNSVNGG
                     FRQVTVRVHAVDRPEATLAQLTGLLSGML"
     gene            complement(5153..5641)
                     /gene="gspH"
                     /locus_tag="VGC69_00395"
     CDS             complement(5153..5641)
                     /gene="gspH"
                     /locus_tag="VGC69_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HEY0293770.1"
                     /translation="MSVRGISERGFTLIEMLVVMVIIGIGATMVTLAIAPGSDRQLRG
                     DAERLVDAFITAQSEARSDGRVILWRADDKGWQFERRARGQTLSTGSNDPVRPDRFER
                     DELLSARSWGQEWPVTLTSQPPLPLAFDTEWIAAPLVLQLQAGADSLTITRNAAGQYE
                     IH"
     gene            complement(5619..6026)
                     /gene="gspG"
                     /locus_tag="VGC69_00400"
     CDS             complement(5619..6026)
                     /gene="gspG"
                     /locus_tag="VGC69_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY0293771.1"
                     /translation="MRRQRGFTLIEIMVVIVIMGILAALIVPRVIDRPDQARAVAAKQ
                     DIASLMQALKLYRLDNGRYPSTAEGLRALAEKPQDAKSWRPYLDRMPSDPWGHPYQYL
                     NPGLKGEIDVFSFGPDGKAGGEHTDAYVGSWDL"
BASE COUNT          963 a         2043 c         2164 g          860 t
ORIGIN      
        1 tggagaatga agcgattgcc ggaaaaccgg gccagcatct cgtgaaacga tgcattcagc
       61 gtaaagaact tgcccggcga aagatcggtg aagtcgctcg cgatccattg ctcataggcc
      121 tggcggcagc gcgccagttg cacggagtcg gccgaaaagg tcggttcgag caggccgccg
      181 cattccacca gcatgcggaa gcgataactt tccgcaagcg cctcgggcgt ttccagcgaa
      241 ggcaggaagg cccagccatg gcccggccgc ttttccacca ggccatcggc cagcatgcgc
      301 atcagggcct tggccagcag gcggcgcgat acgggatagg cgagcagcag atccttctcg
      361 agcacggtgt cgggcagcag ccggcgcgcg cgcgcatcca cgagctgctg gtagagcccg
      421 tcgattccca tgccggactc ctcgaagtcc ggctgttcca tgccctcctg gcgctcgagc
      481 acgaagtagc cgctgttggt gcggtactgg atcagtccgc gacccgccaa gagctgcagc
      541 gccgcccgca ccggcgaacg cgataccttg aattccgcgg ccagcgcttc ttcggtcaga
      601 tggctgccgg gcgcaagctc gccctccacc acctgccgtg cgatgcggcg cgcgatatcg
      661 atttgcagcg gcgaggcgcg cttgggcgcc ggctgtgttc cggaagctga cataggcgtc
      721 gattctatgg cttcccggca atggtgggaa cgctggccct gccagcaaag aaaagttgtc
      781 atggaaccgc ggcaagcgtg ggaaagccga aaacgtgcgc gcccccgcgc cgcggcccga
      841 tgaggttgag caggccgttc aggccggcag cctgctcggc cgtcgcgtcg cccgtggtct
      901 tggcctgccc ctgaaagcgc aaggcgcggc cgtcccaggc gccgtgcccg gtcaggtcca
      961 gcgggccatg ttccgtgctg agatccaggg tgagggcctg gccggtgctg gccacgcgca
     1021 ggcgaaaatc gccgagcgac gcaagcggcg tcaccgccga gctggcgtcg cgccagagca
     1081 ggtcgagcag cgggccggcc gcctggggct ggtgcgtgct caaggcattc cagctcacct
     1141 ccaggaaacc gccgggcccc atggtattga agggcgcgcc cagcgcggtc agcgccgcgg
     1201 cgggcaggcg gacttgctgg gcggagaggc gcatcccgtc ccatcccggc gccaggacca
     1261 gcggaccgcg cagccaggga tggcgcagcg ccatggtcca gccgcgccat tgccactgga
     1321 ccgggtcggt gagcatgcgg cgtgtttcgg gatagccgat ggcgatccag gcctgcccgt
     1381 gccaaatcgt gccatcggca tccaccacgg ccagcgggcc ggaggccggc acccagctca
     1441 gcagccagcg ggccggcagg acgaccgcgg ccgaggcgag cgcgaccacg atggccagca
     1501 gcgacaccgc aatgcgcgag ctgcgcatga ggggatggat cgccgtcaac ccgcgcatcg
     1561 gctatttggc acgcggggcc gtgggttcga gcgtgacgac gccatcgacg cgtccgggca
     1621 ggcggcgctt gtcggcgtcc agggcgcgat cgagttcgac actggcgacg gtcaggcgca
     1681 aatcgcgcga gacgtcatcc agccagcgca gcagcaccag ggcctgggcc tggtccagct
     1741 tcaaagtcca ttgcggcttg gcggcatcgg cactggttgc ctgcaaggac cattgcgcgg
     1801 cgggcaagcc cgattgctgc aggctggact gcaaatcggc gatggtcggc gcggcattgg
     1861 ccatgcgcgc ggcatggcgt tgcaacccgc cggaacgggc ggccagatcg gccacgacgg
     1921 ccgcctgcga ccgcagcttg ggcaactcgg cctgcaactg gccgacggta tccagggcgg
     1981 gctggaccag cagcagccac accaggacgg cgggcaccag cacggccagc ccgatgacca
     2041 gttgccgctc gcgcagcgtg agctgctgca gccatgccgt cacctgggca tgcagggtgg
     2101 cgtagcgcgc ccgcaggccg cgccacgctg gaagcaaggt ctcgctgagg ttcatggctg
     2161 caccggcacg atgcgccaag tggcgctgtt atcgccatgc tcgacccgga tgcccgaggc
     2221 ggaggccttt tgcatgatgg cgggcatgtc gcccaggtga tccgagcctc cctggtcggc
     2281 aagctgcaga tccagcgcct ggtcgcggta gaccagcttg tcgacctggt ccgccgcgaa
     2341 cggcagggcc tgggccccgg cgcgggccag ggcgatgaaa tcgcccggcg tgccgctgct
     2401 gctcccggtg gccagcgcgt catagccttg ctgggcctgg cgcaccggat cgagcacgac
     2461 gggtatgccc gggaaggcgt cgcgcacttg ggtaaccatg gtgtcgcgca gccgcgtggc
     2521 ctcgccgcgc aattgcatgg cgtacaggtt gagccccgcg acccagagca cggccgccgc
     2581 cacggcccag cgccataccg gccgccagtg cgaaacctgc cgcggcgcga gctgcggcaa
     2641 ggcgagcgac caggacggcg tgtcggccag ccagcgcgga tcggtcggtt cgagcaaggg
     2701 cgcgtcgggc gcctggccgc ccccgagcgc ctggacggga atcagcgcgc ggatttccag
     2761 gcccgcttgc gagagcagca gccaggcgcg gcgcagcgcc tccttgtcgg cccaggcgac
     2821 ggtcacctcg ccatcgcgcg aacgcaggcc atggccgacg gccagggagt cgagttcgcc
     2881 gagcaccagg gcttcgagcg cgctgcgcac cgcgtcggga aagcgcgact tgggcacggc
     2941 gggcacttgt accgcggcca cgatggcgtc gtgcgcatgc agcacggcct ggatcggcag
     3001 gcgcggaaaa gcattggcca gcgcggcggc gggcaggctg ccgcgctgca cgcagcggcc
     3061 cgaacggtcg aatcgggcat aagccagcgg cagggaggcc gtcagatcgg ccagcgcggg
     3121 caaggctatg cgcaggaggt tcttcacgat atttcctgag tccagagcag gctggtgccg
     3181 ccgccgacgc tcgacagcag cgcgcgcagc gtcaagcggg cacgctcata ggcgacggtg
     3241 cccgtggcca tgaaccaggc gctggtcacg gtcacttggg gagccgggac ctcggggacc
     3301 aggctggcga tccggttggc gaaatcgccg gtgtccacga accagtgccc ccggtcgcgg
     3361 tctcccgtga ggttgctcgc ctgggcgagc gacaaaccgg gcaccagggc cgcgatgact
     3421 tcgggcggcg cggtgttgac gttgatgacc gtgttgacgg gcagcaccgt catggcgcgg
     3481 gccaacacga gccgcgtggc ctcgtcggcc cccaggaagc cggcgacgtc gtcgatgttg
     3541 cgcggcatgg gcgcggcggc ggggctggcc gcggtattgg cctgggcgga cgcaccggcg
     3601 aggccggccg cggcattggt ctggtcgggc gtgctggcca cgctgcccgt gacgcccgcg
     3661 gctgccggag tccccgcgga ctccaacacg ccgcgcggct gggactgcgc gatcagcgcc
     3721 gccatctgct gggccagcga cgccggcagg ttcaggttgg ccatcaggcg ctgcagcagg
     3781 gcaagctggt ccgccgcgat cacgccgttg gaggcgaggt tgtaaagatt gaattttccc
     3841 tgctcgtctt ccagcatgcc ggagaacagc gcgacccggt cgtcgccggg ctgcgtgatg
     3901 cgggtgtcct gcacgggcac ggcccagatc tggtcgccct gcgtggagga ttcgcggctg
     3961 ctgtgctcgc gcaggatcag ccgcgcccag tcgatgccgc ccgtcaggag ccagcgcgcc
     4021 tgcacgcgcg cctcctcgtt ctcgacctgg cgcaccgtgg cggtctggcg caggaacagg
     4081 ctgctgcaca gcgcggcgac gatcgccacg atgatgagcg cggcgatcac ggcggcgccg
     4141 cgctggcgat gcggatccgt cggctggaac gtcaccatgg caatagcacg atgcggtcga
     4201 aaggctgtac ggcgccttgc cgctggcggg ccagcatgat gtccagcccc gcggcatgct
     4261 gcgccagcga cgcgggcaga tcggcccagc cgcggcccgg cacccaggcg cgcagatgaa
     4321 acgtcgtcgc gttcggcatg atcagcgcgc cggaactcgg ggcgggcaag ggatagcggt
     4381 cgcccgaggc gcccacgtat cgccacaagc catcggcacg caattgccag aggacgtgca
     4441 cccatcgccc cggctgatcg ggcgcggcgc gcacgatgtc cagcgagggc atggccccct
     4501 tgctcatctg gatgccgcct ggcaaggcgc cgtcggcgcc gagcggccgg ggcacgccca
     4561 cataggcctg ctgcacgtcg cgttcgagct gccccagcac gctcacgatc atgtcgttgt
     4621 cctgggcctg ctcatcgatg cgggtatggg cctgcgcgac ccggtccagg ccgcgccagg
     4681 cgagcaggct gaccagcgcc atcagcgcaa tggccaccag cacctcgatc aaggtgaagc
     4741 cttgctggcg cggcagggca tgcccggcgg tcatagcatg cccgacagca ggccggtgag
     4801 ctgcgccagc gtcgcctcgg gccggtcgac ggcatggacc cggaccgtga cctggcgaaa
     4861 gccgccgttt accgaattcg taaaaacgcg ctcgcaccgc aggttccgcc caccctcggg
     4921 gcaaggcacc gtgtcccggc ccggctgggg cacggtctgt tccagccgca gctgcgccag
     4981 gacattctcg gcggccagca aggccagcga cttgtccagc agcgccaggt tgttgtcgat
     5041 catgacgccc gtggcgcgta tggccgcgcc catcgcgatg gcgatgatgg ccagggccac
     5101 caagacttcg atcagcgtga atccccgctg gcgatcccgg gcgcgcgggg cttcagtgga
     5161 tctcatactg gccggccgcg ttgcgggtga tggtcaggct gtcggcgccc gcctgcagtt
     5221 gcaggaccag cggcgcggcg atccattcgg tgtcgaaggc cagcggcaac ggcggctggc
     5281 tcgtcaaggt aacaggccac tcctgccccc aggaccgggc ggacagcagc tcgtcccgct
     5341 cgaagcggtc ggggcggacc gggtcgttgc tcccggtgga caaggtctgc ccgcgcgccc
     5401 tgcgttcgaa ctgccagccc ttgtcgtcgg cccgccacag aatcacgcgg ccgtcgctgc
     5461 gcgcctcgct ctgggccgtg atgaaggcat cgaccaggcg ctcggcgtcg ccgcgcagct
     5521 ggcgatccga gccgggtgct atcgcaagcg tcaccatcgt ggcaccgatg ccaatgatca
     5581 ccatgaccac cagcatctcg atgagcgtga agccgcgttc agagatccca cgaaccgaca
     5641 taggcatcgg tgtgctcgcc gcccgccttg ccgtcgggac cgaacgaaaa cacgtcgatc
     5701 tcacccttca ggcccgggtt gaggtattgg tagggatggc cccagggatc gctgggcatg
     5761 cggtcgagat aaggccgcca ggacttggcg tcctggggtt tctcggccag ggcgcgcagc
     5821 ccttcggcgg tgctggggta gcggccgttg tccaggcggt acagcttgag cgcctgcatg
     5881 aggctggcga tgtcctgctt ggctgcaacc gcccgggcct ggtcggggcg atcaatcacg
     5941 cgcggcacga tcagggcggc caggatgccc atgatcacga tcaccaccat gatctcgatc
     6001 agggtgaagc cccgttgacg gcgcaggggg
//
LOCUS       DASWPO010000011         8217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2055973, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000011 DASWPO010000000
VERSION     DASWPO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055973"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VGC69_00405"
     CDS             <1..1520
                     /locus_tag="VGC69_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0293772.1"
                     /translation="LVAIGLQPGERIGIWSPNNAEWVITQFATAKAGLILVNINPAYR
                     TVEVEYALNKVGCKLLVTMATFKTSDYLAMLRELAPELATARLGELQAARLPQLRRVV
                     WIDKAGQGADEPGVQRFSDLLASGDPADAKVRQAQQGLKNTDPINIQFTSGTTGFPKG
                     ATLTHRNILNNGFFIGECMKLTADDRLCIPVPLYHCFGMVLGNLACLTHGSAIVYPND
                     GFDPLLVLEAVQAERCTGLHGVPTMFIAELDHPRFAEFDLSTLRTGIMAGSPCPIEVM
                     KRVVGLMHMSEVTIAYGMTETSPVSCQSATDTPQEKRVSTVGLVHPHLEVKIIDPGTG
                     ATVPRGTPGELCTRGYSVMHGYWDDEAKTREAIDTERWMHTGDLATMDDEGYVNIVGR
                     IKDMVIRGGENLYPREIEEFLYRHPAIQDVQVVGVPDPKYGEELCAWIIVKSGQTLAE
                     DAVRDFCKGQIAHQKIPRYIRFVSEFPMTVTGKIQKFKIREAMSDELNLVEAKTA"
     gene            1581..2684
                     /locus_tag="VGC69_00410"
     CDS             1581..2684
                     /locus_tag="VGC69_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293773.1"
                     /translation="MLRLRRPKLAGDALGAAAFSWQAYKAPMLAPDQWVPDAPVPLAR
                     LDASLAWNAARQKAPRLHDGFAQRMREELKAKLEDNTIYRLFEARATDGVPAFTCGLA
                     TYFDHIDSGEALVWEAAQARVKAGKKAGQAELLKAMLPHMPLRQKTGPLDFSTRTCCI
                     GVDTLTVVGFRNRPAVFFMHERGSGQRGPAEAGNSLHVVPAGSFQPNAAKDLHHDAEY
                     SVRRNVLRECAEELFDKEDLMQASQRLSPDRIFEKDPELHVLKGMFDDGGITQWYLGS
                     FIDPLTLKVEVLTLLLVDGDRLADSFEALRDGWEGHHDQYPFTVEELDDVQAQPRLLP
                     AGQAALILGIRHYGWIQQKLAAQLAAQAPATVA"
     gene            2979..3176
                     /gene="rpmI"
                     /locus_tag="VGC69_00415"
     CDS             2979..3176
                     /gene="rpmI"
                     /locus_tag="VGC69_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY0293774.1"
                     /translation="MPKMKTKKSAAKRFQVRGSGSIKRGQAFKRHILTKKTTKNKRHL
                     RGSAAVHETNLASVKAMLLIA"
     gene            3189..3548
                     /gene="rplT"
                     /locus_tag="VGC69_00420"
     CDS             3189..3548
                     /gene="rplT"
                     /locus_tag="VGC69_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY0293775.1"
                     /translation="MPRVKRGVTARARHKKVLTAAKGYRGRRGNVFRIAKQAVMRAGQ
                     YAYRDRRNKKRTFRALWITRINAAVREQGMSYSVFIAGLKKAAIELDRKVLADLAVRD
                     KAGFAAIVQQAKAALGA"
     gene            3715..4737
                     /gene="pheS"
                     /locus_tag="VGC69_00425"
     CDS             3715..4737
                     /gene="pheS"
                     /locus_tag="VGC69_00425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY0293776.1"
                     /translation="MSTSLDDLVAQAEERFAQASDAAALENAKARFLGKEGALTVLLK
                     GLGALSAEEKRDTGARINQAKQKIEASLNARRAALAQAELDARLAAETIDVTLPGRGK
                     LPGGIHPVIRTWERVEQIFRSIGFDVASGPEIENDWTNFTALNSPPDHPARSMQDTFY
                     VDMNGSDGQPLLLRTHTSPMQVRYARMNQPPIKVIAPGRTYRVDSDATHSPMFHQVEG
                     LWIAEDISFADLKGVYTDFLRCFFESDDLVVRFRPSFFPFTEPSAEIDMMFTSGPNKG
                     RWLEISGSGQVHPQVVRNFGLDPERYIGFAFGSGLERLTMLRYGVNDLRQFFEGDLRF
                     LRQFNE"
     gene            4786..7239
                     /gene="pheT"
                     /locus_tag="VGC69_00430"
     CDS             4786..7239
                     /gene="pheT"
                     /locus_tag="VGC69_00430"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0293777.1"
                     /translation="MQFPESWLRTLVNPKLSTDELAHKLTMAGLEVDDLAPAVPPFSG
                     VVLARIEEIAPHPDADKLRVCKVDDGSGQLLQIVCGAPNAAAGITVPLARIGAALPGG
                     IQIGKAKMRGVESFGMLCSARELGLSQDHGGLMLLPGSLAPGADFRQALDLEDMLFTL
                     KLTPNRADCLSILGVAREVAALTGAPLTLPEMKPVAVTTDARLSVKVSAPDLCGRFAG
                     RVVRGVDAHAASPDWMRTRLERAGMRSISALVDISNYVMLELGRPTHVFDLDKVPGDI
                     EVRWAREGETLELLNGQTVALDGKVGVVTAAGRVESLAGIMGGESTSVTLDTRNIYIE
                     AAFWWPEAIAGRSRRFKFSSEASHRFERGVDYASIPEHLERITALILEICGGQAGPVD
                     DQVLQLPERKPVRMRLARCQRVLGVAVPGDEVADIFTRLGLPFTRESTRTGDDFIVSP
                     PSYRFDLEIEEDLIEEVARIHGFESIPDVPPMARAKMRAEPETHRGLHALRRLIAARD
                     YQEVVNYSFVEADWERDYAGNGEPVRLLNPIASQMSVMRSSLIAGLVANIRHNANRKQ
                     TRVRVFELGGVFLRDAQVQDGPLSVAGVSQPMRLAGAAWGPAVAEQWGEASRHVDFYD
                     AKMDVEALFGAAAESSQTRLRFTAARHPALHPGRSARIEFDGKTVGWIGELHPRWAQQ
                     AELAHAPVVFELDAVALQAGTMPVVGEISRQPVVQRDLALWVKADMPAQALLDTVAAA
                     VEADARLAVVQDVRLFDVWRDKQQGADAGDAKPAEKSLAFRFWLQDTEVTLDEARVSD
                     CMTRILDALSAAHGARLRA"
     gene            7275..7607
                     /locus_tag="VGC69_00435"
     CDS             7275..7607
                     /locus_tag="VGC69_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY0293778.1"
                     /translation="MLAEPRTLTKAELAELLFERVGLNKREAKDIVDTFFEEIREALA
                     RGDSVKLSGFGNFQVRDKPPRPGRNPKTGETIPIAARRVVTFHASQKLKSVVEQTPSD
                     GEPDFDDE"
     gene            7719..8114
                     /locus_tag="VGC69_00440"
     CDS             7719..8114
                     /locus_tag="VGC69_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0293779.1"
                     /translation="MTRPENTAVTLPPIPAKRYFTIGEVSDLCGVKPHVLRYWEQEFT
                     QLKPVKRRGNRRYYQHHEVLLIRRIRSLLYEQGFTISGARNRLGDTRDAVANDPDAAV
                     RLSPAELQSLRTELADIAELLDEAAKASA"
BASE COUNT         1472 a         2800 c         2637 g         1308 t
ORIGIN      
        1 ggctggtggc gatcggactg caaccgggcg agcgtatcgg catctggtcg ccgaacaacg
       61 cggaatgggt gatcacgcag ttcgccaccg ccaaggccgg gctgatcctg gtcaacatca
      121 atccggccta ccgcacggtc gaggtcgaat acgcgctcaa caaggtgggc tgcaagctgc
      181 tggtgaccat ggccaccttc aagaccagcg actacctggc catgctgcgc gagctggccc
      241 ccgagctggc cacggccagg ctgggcgagt tgcaggccgc gcgcctgccg cagctgcgcc
      301 gggtggtgtg gatcgacaag gcggggcaag gggcggacga gcccggcgtg cagcgctttt
      361 ccgatctgct ggccagcggc gacccggccg atgcgaaagt gcgccaggcg caacagggcc
      421 tgaagaacac cgacccgatc aatatccagt tcaccagcgg caccacgggg tttcccaagg
      481 gcgccacgct cacgcaccgc aatatcctca acaacggctt tttcatcggc gagtgcatga
      541 agctcacggc cgacgaccgc ctgtgcatac cggtgccgct ctaccattgc ttcggcatgg
      601 tgctgggcaa cctggcgtgt ctcacgcacg gctcggccat cgtctatccc aacgacggct
      661 tcgacccctt gctggtgctg gaagccgtgc aggccgaacg ctgcaccggc ctgcacggcg
      721 tgcccaccat gttcatcgcc gaactcgacc acccgcgctt tgccgagttc gacctgtcca
      781 cgctgcgcac cggcatcatg gccggctcgc cgtgccccat tgaggtgatg aagcgggtgg
      841 tgggcctgat gcacatgagc gaggtcacca ttgcctacgg catgaccgag accagccccg
      901 tcagttgcca gagcgccacc gacaccccgc aggaaaagcg cgtctccacg gtgggactgg
      961 tgcacccgca cctggaagtg aagatcatcg acccgggcac cggcgccacg gtgccgcgcg
     1021 gcacgccagg cgagctgtgc acgcgcggct attccgtcat gcacggctac tgggatgacg
     1081 aggccaagac gcgcgaggcc atcgacaccg agcgctggat gcacaccggc gacctggcca
     1141 cgatggacga cgaaggctat gtgaacatcg tcggccgcat caaggacatg gtcattcgcg
     1201 gcggcgaaaa cctttatccg cgcgagatcg aggagttcct ctaccgccac ccggccatcc
     1261 aggacgtgca ggtggtgggc gtgcccgacc cgaaatacgg cgaggagctt tgcgcctgga
     1321 tcatcgtcaa gtcgggccag acgcttgccg aggacgccgt gcgcgatttc tgcaagggcc
     1381 agatcgccca ccagaagatc ccgcgctaca tccgcttcgt gagcgagttc cccatgacgg
     1441 tgacgggcaa gatccagaag ttcaagatcc gcgaggccat gagcgacgag ctcaatctgg
     1501 tcgaagcgaa gacggcctga aaacaggctg gggcggcatg gcgcgcgcgc accggaaacg
     1561 gtgcgcgcgc ttcgttacaa atgctacgat tgcggcgccc caaactcgcc ggcgacgccc
     1621 tgggcgccgc ggcgttcagc tggcaggcct acaaggcccc catgctggct ccggaccagt
     1681 gggtgcccga cgcgcccgtg ccgctcgcgc gcctggacgc aagcctggcc tggaacgcgg
     1741 ccaggcagaa ggcgccgcgc ctgcacgacg gtttcgcgca gcgcatgcgc gaagagctca
     1801 aggccaagct cgaggacaac accatttacc gcctgttcga ggcgcgggcc accgacggcg
     1861 tgccggcctt cacttgcggg ctggccacgt atttcgacca tatcgattcg ggcgaggcgc
     1921 tggtctggga ggccgcgcag gcccgcgtga aggccggcaa gaaggccggg caggcggaac
     1981 tgctcaaggc catgctgccg cacatgccgc tgcgccagaa gaccgggcca ctggattttt
     2041 ccacccgcac ctgctgcatt ggcgtcgata ccctgaccgt ggtgggtttt cgcaaccgcc
     2101 cggcggtgtt cttcatgcat gagcggggct cgggccagcg cggcccggcc gaagcgggca
     2161 attcgctgca cgtcgtgccg gccggctcct tccagcccaa cgccgccaag gacctgcacc
     2221 acgacgccga atacagcgtg cgccgcaatg tgctgcgcga atgcgccgag gagctcttcg
     2281 acaaggaaga cctcatgcag gcctcgcagc gcctgtcgcc ggaccggatc ttcgagaagg
     2341 atccggagct gcacgtgctc aagggcatgt tcgacgacgg cggcattacc cagtggtacc
     2401 tgggcagctt catcgacccg ctgacgctga aggtggaggt cctgaccctg ctgctggtcg
     2461 acggcgaccg gctggccgac tctttcgagg ccctgcgcga cggctgggaa gggcatcacg
     2521 accagtaccc cttcacggta gaggaactgg acgacgtcca ggcccagccg cgcctactgc
     2581 cggcggggca ggccgcgctc atcctgggca tcaggcacta cggctggatc cagcagaaac
     2641 tggcggcgca actggccgcc caggcgcctg ccacggtggc ttgaatattg gtgacatatc
     2701 tcttccgcgg gggaggaggc gccgactggt cacagggctc gacaagttgg acaaaaatgc
     2761 catacaatca aaggctttcc cggatttgac gctgttatcc tgaggtatat cgggcatcgg
     2821 ctttccggga aaaccacggt gacacgggtc aggcaggcat gccgcaagcc ctgagcattt
     2881 tttgcccagg cacctgtgat caccgacaac tcaccggccc cagggatggt tttaagccgt
     2941 atcccgaagg gtaagaaact ggaaaaacag gaatcatcat gcccaagatg aaaaccaaga
     3001 aaagcgctgc caagcgcttt caggttcgcg gcagcggatc gatcaagcga ggtcaggcgt
     3061 tcaagcgtca catcctgacc aagaagacca ccaagaacaa gcgccacctg cgcggttccg
     3121 cggccgtcca tgaaaccaat ctcgcctccg tcaaggcgat gttgctcatc gcttgatagg
     3181 agtccactat gcctcgcgtt aaacgtggtg ttaccgcccg tgcccgtcac aagaaagtcc
     3241 tgaccgcagc caagggttat cgcggccgcc gcggtaatgt tttccgtatc gccaagcagg
     3301 cggtcatgcg tgccggccag tatgcctacc gcgaccgccg caacaagaag cgcaccttcc
     3361 gcgccctgtg gatcacccgc atcaacgccg ccgtgcgtga acagggcatg agctacagcg
     3421 tgttcatcgc cggcctgaag aaggccgcga tcgaactcga ccgcaaggtg ctggccgacc
     3481 tggccgtgcg tgacaaggcc ggcttcgccg ccatcgtgca acaggccaag gcggccctgg
     3541 gcgcctaagc caggcaagca ggccaagcaa gcctgactga taccggcgcg ctttatcgcg
     3601 taccgtgctg caaatggggc tggggttgcg ttctccagcc ccatttgcat ttccagggcc
     3661 cgaagcgtgc cgcccacggc cctgtccatc gactcgacaa gttttgcgac gcccatgagc
     3721 acatcgctgg atgacctggt tgcacaggcc gaagaacgtt tcgcgcaagc ctccgatgcg
     3781 gccgcgctgg aaaacgccaa ggcgcgtttc ctcggcaaag agggcgccct gacggtgctg
     3841 ctcaagggcc tgggtgcgct ttccgccgag gaaaagcgcg ataccggcgc ccgcatcaac
     3901 caggccaagc agaagatcga ggcctctctc aatgcgcgcc gcgccgccct ggcgcaggcc
     3961 gagctcgacg cccgcctggc cgccgagacc atcgacgtca cgctgcccgg ccgcggcaag
     4021 cttcccggcg gcatccaccc ggtgatccgc acctgggagc gggtcgagca gattttccgt
     4081 tccatcggtt tcgacgtggc cagcggcccc gagatcgaga acgactggac caacttcacc
     4141 gccctgaaca gcccgccgga ccaccccgcg cgctcgatgc aggacacctt ctacgtcgac
     4201 atgaacggca gcgacggcca gccgctcttg ctgcgcacgc acaccagccc catgcaggtg
     4261 cgctacgccc gcatgaacca gccgcccatc aaggtcatcg cgccgggccg cacctaccgc
     4321 gtcgacagcg acgccacgca ttcgcccatg ttccaccagg tcgagggcct gtggatagcc
     4381 gaggacatct cgttcgccga cctgaagggc gtgtacaccg acttcctgcg ctgcttcttc
     4441 gagagcgatg acctggtggt gcgctttcgc ccgtccttct tccccttcac cgagccctcg
     4501 gccgagatcg acatgatgtt cacgtccggc cccaacaagg gccgctggct ggagatctcg
     4561 ggttcgggcc aggtgcatcc gcaggtggtg cgcaacttcg ggctcgaccc cgaacgctat
     4621 atcggttttg ccttcggttc gggcctggag cgcctgacca tgctgcgcta cggggtcaac
     4681 gacctgcgcc agttttttga gggcgacctg cgcttcctgc gccagttcaa cgaataagtc
     4741 gatcgacgca accctcaacg tcccaacgaa aaagcacggc tgaacatgca atttcccgaa
     4801 tcctggttgc gtacgctggt caatcccaag ctgtccaccg acgagctcgc gcacaagctg
     4861 accatggccg gcctcgaggt cgacgatctc gcgcccgccg tgccgccgtt ctccggcgtg
     4921 gtgctggccc gcatcgaaga aatcgccccg caccccgacg ccgacaagct gcgcgtgtgc
     4981 aaggtcgacg acgggtccgg ccagttgctg cagatcgtct gcggcgcgcc caacgcggcc
     5041 gccggcatca ccgtgccgct ggcgcgcatc ggcgccgcgc ttccgggcgg catccagatt
     5101 ggcaaggcca agatgcgcgg cgtcgagtcc ttcggcatgc tgtgctcggc gcgcgaactg
     5161 ggcctgtcgc aggaccacgg cggcctgatg ctgctgcccg gatcgctcgc gcccggcgcg
     5221 gatttccgcc aggcgctcga cctggaagac atgctcttca cgctcaagct cacgcccaac
     5281 cgcgccgact gcctgtccat cctgggcgtg gcgcgcgagg tggccgcgct caccggcgcg
     5341 cccctgacgc tgcccgagat gaagcccgtg gccgtcacca ccgatgcccg cctgtccgtg
     5401 aaggtgagcg cgcccgacct gtgcggccgc ttcgccggcc gcgtggtgcg cggcgtcgac
     5461 gcccacgccg cctcgcccga ctggatgagg acgcgcctgg agcgcgccgg catgcgctcg
     5521 atctcggcgc tggtggacat ctccaactac gtgatgctcg agctgggccg tcccacgcac
     5581 gtgttcgacc tggacaaggt gccgggcgac atcgaggtgc gctgggcccg cgaaggcgaa
     5641 accctggaac tgctcaacgg ccagaccgtg gcgctcgacg gcaaggtggg cgtggtcacg
     5701 gccgccggcc gcgtcgaaag cctggccggc atcatgggcg gcgagtccac ctcggtgacg
     5761 ctggacacgc gcaacatcta tatcgaggcg gccttctggt ggcccgaggc catcgccggc
     5821 cgctcgcgcc gcttcaagtt cagctccgag gccagccacc gcttcgagcg cggcgtggac
     5881 tacgccagca tccccgaaca cctcgagcgc atcaccgcat tgatcctgga gatctgcggc
     5941 ggccaggccg gcccggtgga cgaccaggtc ctgcaactgc ccgagcgcaa gcccgtgcgc
     6001 atgcgcctgg cgcgttgcca gcgcgtgctg ggcgtggcgg tgccgggcga cgaagtcgcc
     6061 gacatcttca cccgcctggg tctgccgttc acgcgcgaat cgaccaggac gggcgacgat
     6121 ttcatcgtgt cgccgccgtc gtaccgtttc gacctcgaga tcgaggaaga cctcatcgaa
     6181 gaggttgccc gcatccacgg tttcgagagc attcccgacg tgccgcccat ggcgcgcgcc
     6241 aagatgcgcg ccgagcccga gacgcatcgc ggcctgcacg cgctgcgccg cctgatcgcc
     6301 gcgcgcgact accaggaagt ggtcaactac agcttcgtcg aggccgactg ggagcgcgac
     6361 tacgcgggca acggcgaacc cgtgcgtctg ctcaacccca tcgcgagcca gatgtcggtg
     6421 atgcgttcga gcctgatcgc cggcctggtg gccaatatcc gccacaacgc caaccgcaag
     6481 cagacgcgcg tgcgcgtgtt cgagctgggc ggcgtgttcc tgcgcgatgc gcaggtgcag
     6541 gacggcccgc tttccgtcgc cggcgtgagc cagcccatgc gcctggccgg cgcggcctgg
     6601 ggcccggccg tggccgagca gtggggcgag gcctcgcgcc acgtggactt ctacgacgcg
     6661 aagatggacg tcgaggcctt gttcggcgcg gccgccgagt ccagtcaaac ccgcctgcgc
     6721 ttcacggcgg ccaggcatcc cgccctgcat ccgggccgca gcgcccgcat cgagttcgac
     6781 ggcaagaccg tgggctggat cggcgaactg cacccgcgct gggcgcagca ggccgaactg
     6841 gcgcatgcgc cggtggtgtt cgaactcgac gccgtcgcgc tgcaggccgg caccatgccg
     6901 gtggtgggcg agatctcccg ccagcccgtg gtgcagcgcg atctggcgct ctgggtcaag
     6961 gccgatatgc ccgcccaggc cctgctcgac accgtcgccg cggcggtcga ggccgatgcc
     7021 agactggccg tggtgcagga cgtgcgtctt ttcgatgtat ggcgcgacaa gcagcagggc
     7081 gccgatgccg gcgatgcgaa acccgccgag aaaagtcttg ccttccgttt ctggctgcaa
     7141 gacactgaag tcacgctgga cgaagcgcgc gtgtccgatt gcatgacgcg catcctggac
     7201 gccttgtcgg ccgcgcacgg cgcgcgcctg cgggcctgac ccaaacgcgc gcgacacaag
     7261 aacaggggat caagatgctt gccgaaccgc gtacgctcac caaggccgaa ctggccgaac
     7321 tgctcttcga gcgggtcggc ctgaacaagc gcgaagccaa ggacatcgtc gacactttct
     7381 tcgaggaaat ccgcgaggcg ctggcccgtg gcgattcggt caagctgtcg ggctttggca
     7441 atttccaggt gcgcgacaag ccgccgcgcc cgggccgcaa ccccaagacg ggcgaaacca
     7501 tacccatcgc ggcgcgccgg gtggtgactt tccacgccag ccaaaagctg aaaagcgtgg
     7561 tcgagcagac tccctcggac ggcgaacccg acttcgacga cgaataaaca acccctgccc
     7621 gggggcgcgg cccgcttgtg cgcaagcccg ggcagtagtg atacgctttg tcggtaattg
     7681 tcatcggctt tcccggacat cgcggcataa ttctttccat gactcgtcct gaaaacactg
     7741 ctgtcacgct tcctcccatt cccgccaaac gctatttcac gatcggcgag gtcagtgatc
     7801 tatgcggcgt caagccgcat gtgctgcgct actgggaaca ggaattcacc caactcaaac
     7861 cggtcaagcg tcgtggcaac cggcgttatt accagcacca cgaagtgctg ttgatacgcc
     7921 gcatccgttc gcttttgtac gagcagggtt tcaccatcag cggggcgcgc aatcgcctgg
     7981 gcgatacgcg cgatgcggtg gcgaatgatc cggacgcggc ggtgcgtctc agcccggccg
     8041 agttgcagtc cttgcggacc gagctggcgg atatcgccga gttgctggac gaggcggcca
     8101 aggcgtctgc ttgattgcct agtttgttcg tttgagttct tgagtcgcgc aggcggttcg
     8161 ggcccggcgc ccggacagta ggaggaaggg cagccccgca ggggccgagg ccccgtc
//
LOCUS       DASWPO010000012         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_717292, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000012 DASWPO010000000
VERSION     DASWPO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717292"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1031
                     /locus_tag="VGC69_00445"
     CDS             216..1031
                     /locus_tag="VGC69_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0293780.1"
                     /translation="MSEEKTRKTAAGAAADGKETGTVRRVLLLLRCLVDHPGESAQSL
                     AARLNLPRSSVHRLLATLRENDFAMNEEGGSFGPGLELYRMAGRLGARMPYRKLAEPL
                     LGELSAKFRETSLLTLLARRQLKMFHAASGSPADPMRYDIQLNRLEPLVWGATARVLL
                     AHLSPAEIDQALMGQDRSPVSGRLPDEAEIQASLQAIRETGHAITHSHRTSNTVGVAV
                     PFFDAEGQVAGSMGFLIPEFRWAQADPEQIVGALSQASGRLSRQLGHSGDAGR"
     gene            complement(1077..2009)
                     /locus_tag="VGC69_00450"
     CDS             complement(1077..2009)
                     /locus_tag="VGC69_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0293781.1"
                     /translation="MSVVPPRRKSLDAPGASLLLVLCLCWSLQQIAVKVAAPDITPAL
                     QIVLRSLLTSLALGALVLRREGWRAFGDGTLVPGLLAGLLCAGEFLLMSQAVLYTTAG
                     HSIAFIYTSPIFAALGLHFLVPEEHMSGRQWCGVALAFAGIVLAFLGKRGAAPDAGNM
                     LLGDLFSLAAGALWGFTTVVIRGSSLSDAAPDKTLFYQMLVTSLAATALYLAGGAPRP
                     VWSTAALLSLAYLTLIVSLWSFLMWFWMMRRYLATQMSAMSFLAPIVGVIFGALLLDE
                     PLEANFVGGSLLALAGILVVTRTRGAAPKAAAAD"
     gene            complement(2019..2645)
                     /locus_tag="VGC69_00455"
     CDS             complement(2019..2645)
                     /locus_tag="VGC69_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY0293782.1"
                     /translation="MTAVTERRVPQKRKPSRIAEERRRNLIDAAIRNISSQGYNAVTI
                     ATICEEAGFSRGLIGHYFSGKDALLLEAVKSVAAELGQAIRDSVHAAPDPEAKLHAVV
                     KASFTPPGFTSDKVAVWVSLAGAARWSTPLGEIYREIWHDYRAGVGRLFERAAQAAKT
                     DLDPIASALTFSQMIEGFWIGWAADPKAVTPKAAAHCCHAYLDLLLRK"
     gene            complement(2751..3164)
                     /locus_tag="VGC69_00460"
     CDS             complement(2751..3164)
                     /locus_tag="VGC69_00460"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0293783.1"
                     /translation="MSLEFILPEGWSRPRGFTHGVTGQDRRVLCVAGQLAGATGADAP
                     PAGMSLPAQFGASLANVVRVVQAAGGQAGDIAALRVFVTDIQAFKQGQAEIAAAWGKL
                     LGRHFPAMTLVEVRALFEETAQVEIEAQALLPGQG"
     gene            complement(3204..>3518)
                     /locus_tag="VGC69_00465"
     CDS             complement(3204..>3518)
                     /locus_tag="VGC69_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293784.1"
                     /translation="GYIRKLMNEGPVTLRQLYEIYALARGGNFIVGTPKDIADMMEEW
                     FVGKAADGFMIGLAHVPSDAQAFVEGVVPALRKRGLFRLAYEGTTLRDHLGLARPASP
                     YA"
BASE COUNT          612 a         1127 c         1191 g          588 t
ORIGIN      
        1 ggtgcgtcct ttgaaagtca attccatgtc tcgtctctct tctgtttcgt gttgggataa
       61 gccagacttt tctttttctt gaatgtctca ttaaatgaga ctatagtccg ttgaatgata
      121 attctcgatc gatagcgcag atgtgtcaac ctgtgatttg tatcgagcgg gccgccgggt
      181 ctcctacaat ccgggcggcg agcgggaagg catagatgag cgaagagaaa actcggaaga
      241 cggcggcagg ggccgcggcg gacggcaagg aaacgggcac ggtccggcgg gtgctgctgt
      301 tgctgcgctg cctggtggac catccgggcg aaagcgcgca gagcctggct gcgcggctca
      361 acttgccgcg cagctccgtg caccgcctgc tggccaccct gcgcgagaac gatttcgcca
      421 tgaacgaaga gggcggctcg ttcggcccgg gattggagct ttaccgcatg gccggccggc
      481 tgggtgcgcg catgccctac cgcaagctgg ccgagccgct gctgggcgaa ctgtccgcga
      541 agttccgcga gacttccctg ttgacgctgc tggcgcggcg gcagctcaag atgttccacg
      601 cggccagcgg ctcgcccgcc gatcccatgc gctacgacat ccagctcaac cggctcgaac
      661 ccctggtctg gggcgccacg gcgcgcgtgt tgctggcgca cttgagcccg gcggaaatcg
      721 accaggcgct gatggggcag gaccgctcgc ccgtgtcggg ccgcctgccc gacgaagcgg
      781 aaatccaggc atcgctgcaa gccattcgcg agacgggcca cgccatcacg cattcgcacc
      841 gcacgtccaa cacggtgggg gtggccgtgc ctttcttcga cgcggaaggg caggtggcgg
      901 gcagcatggg cttcctgatc cccgagttcc gctgggccca ggcggatcca gagcagatcg
      961 tcggcgcgct cagccaggcg agcgggaggc tctcgcgcca actgggacac tcaggcgacg
     1021 cgggccggtg agcgtggcgg tgccggcggc gctgtcgatt tgcccggggc ggcaggctaa
     1081 tccgccgccg cggccttcgg ggcggcgccc ctggtcctgg tcacgaccag aatgcctgcc
     1141 agcgccagca gcgagccgcc cacaaaattg gcttccagcg gctcgtcgag cagcagcgct
     1201 ccgaagatca cgccgactat cggcgcgagg aaggacatcg ccgacatctg cgtggccagg
     1261 tagcggcgca tcatccagaa ccacatcagg aagctccaca ggctgacgat gagcgtcagg
     1321 taggccaggc tgagcagggc ggccgtcgac cagacgggac gcggcgcgcc gccggccagg
     1381 tacagggccg tcgcggccag gctggtcacc agcatctggt agaacagcgt cttgtcgggc
     1441 gccgcgtcgg acagcgacga gccgcgtata acgaccgtgg taaagcccca tagggccccc
     1501 gccgcgaggc tgaacaagtc gcccagcagc atgttgccgg cgtccggggc tgcgccccgc
     1561 ttgcccagga aagccagcac gatgccggcg aaggccaggg ctacgccgca ccattgcctg
     1621 ccgctcatgt gttcttcggg aaccaggaag tgcaggccga gcgccgcgaa aatgggcgag
     1681 gtgtagataa aggcaatgct gtgcccagcg gtggtgtaga gcacggcctg ggacatcagc
     1741 aggaattctc ccgcgcacag caggccggcc aacaagccgg ggaccagggt gccgtcgccg
     1801 aaagcgcgcc agccctcgcg ccgcagcacc agggcgccaa gcgcgaggct cgtcagcagt
     1861 gagcgcagga cgatttgcag cgcgggcgta atgtcgggcg cggcgacctt gacggcaatt
     1921 tgctgcaggc tccagcacag gcacagcacc agcagcaggc tggcgccggg cgcatcgagc
     1981 gatttacgcc gcgggggaac gacggacatt gcggcgcact acttgcgcag cagcaaatcg
     2041 agataggcgt ggcagcaatg ggcggcggcc ttgggcgtca cggccttggg gtcggcggcc
     2101 cagcctatcc agaaaccctc gatcatttgc gaaaaggtga gcgcgctggc gatgggatcc
     2161 aggtcggttt tcgcggcttg ggcagcgcgt tcgaaaagcc ggccgacgcc tgcccggtag
     2221 tcgtgccaga tctcgcggta gatttcgccc aagggcgtgg accagcgcgc ggcgccggcc
     2281 agcgacaccc acacggcaac cttgtcggag gtgaagccgg gcggcgtgaa gctggccttg
     2341 accacggcat gcagcttggc ttcgggatcc ggcgcggcat gaacggagtc gcggatggcc
     2401 tggcccaatt ccgcggccac gctcttgacc gcctcgagca gcagcgcgtc cttgcccgag
     2461 aaatagtggc cgatcaggcc ccgggaaaag ccggcttctt cgcagatcgt ggcaatggtg
     2521 acggcgttgt agccttgtga cgaaatattc cgtatggcgg catcgatcag gttgcggcgt
     2581 cgttcttccg caatgcgcga gggtttgcgt ttttgtggga cccttcgctc ggtaactgcc
     2641 gtcataagtg tctctgtcct tcttttttgg cgcgagtgtt cccgcacata ttaacccgtg
     2701 ctccgggcgc cctcatatca tgaaaatcaa gggcatatcc catatctttc tcagccttgc
     2761 ccgggcagca gcgcctgggc ttcgatttcc acttgggcgg tttcttcaaa gagcgcgcgg
     2821 acttcaacca aggtcatggc cgggaaatgc cgccccagca gcttgcccca tgcggccgcg
     2881 atttccgcct gcccctgctt gaaggcctgg atatcggtga cgaaaacccg caaggcggcg
     2941 atatcgccgg cctggccgcc ggcggcctgc acgacgcgaa ccacgttggc gagcgaggcg
     3001 ccgaattgag cgggcaggct catgcctgcc ggcggggcgt cggccccggt cgcgccggcc
     3061 agttggccgg caacgcacag cacgcgccgg tcttgccccg tgacaccgtg cgtgaacccg
     3121 cgtgggcggg accagccctc gggcagtatg aattccaggc tcatggttgt ctccttattg
     3181 gtttgccgcg gtcagggcag cgttcaagca tatggcgaag ccggcctggc caggccgagg
     3241 tgatcgcgca gcgtcgtgcc ttcgtaggcc agccggaaca gtccgcgctt gcgcagcgcc
     3301 ggcacgacgc cctcgacgaa ggcctgggcg tcgctcggca cgtgcgccag gccgatcatg
     3361 aagccgtccg cggccttgcc gacgaaccac tcctccatca tgtcggcaat gtccttcggc
     3421 gtgcccacga tgaagttgcc gccgcgcgcc agcgcataga tttcgtacag ctggcgcaag
     3481 gtgaccgggc cttcgttcat cagcttgcgg atatagcc
//
LOCUS       DASWPO010000013        11021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2324155, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000013 DASWPO010000000
VERSION     DASWPO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324155"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..733)
                     /gene="pyrH"
                     /locus_tag="VGC69_00470"
     CDS             complement(14..733)
                     /gene="pyrH"
                     /locus_tag="VGC69_00470"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0293785.1"
                     /translation="MTSSRSYKRVLLKLSGEALMGEDAYGINRGTIIRMTQEIAEVAA
                     TGIELAIVIGGGNIFRGVAPGAQGMDRATADYMGMMATIMNSLALQDALKHQGVDARV
                     QSALNIEQVVEPYIRPKALRYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEIGAEIVL
                     KATKVDGIYSADPNKDPTATRYARISFDEAIVRRLEVMDATAFALCRDQKLPIKVFSI
                     NKPGALKRAVAGEDEGTLVHV"
     gene            complement(915..1793)
                     /gene="tsf"
                     /locus_tag="VGC69_00475"
     CDS             complement(915..1793)
                     /gene="tsf"
                     /locus_tag="VGC69_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY0293786.1"
                     /translation="MAEITASLVKELREKTDAPMMECKKALTEAEGDLARAEEILRVK
                     LGNKASKAAARVTAEGLIGLFISADGKQGAVIEVNCETDFVGKNNDFVAFVNGLAKLV
                     AEKNPANVDALSALPYGEGTVETTRTALIGKIGENMSIRRFERIQTPNALASYVHGGR
                     IGVLVEFTGADEVGKDLAMHIAATKPKALSADGVPAADIATERSVAEQKAAESGKPAE
                     IVAKMVDGSIAKFLKEVTLLSQPFVKNDKQTIEQMLKEKNASISKFVLFVVGEGIEKK
                     VTDFAAEVAAAAAGQA"
     gene            complement(1941..2690)
                     /gene="rpsB"
                     /locus_tag="VGC69_00480"
     CDS             complement(1941..2690)
                     /gene="rpsB"
                     /locus_tag="VGC69_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY0293787.1"
                     /translation="MSLMREMLEAGVHFGHQTRYWNPKMAPYIFGSRNKIHIVNLEKT
                     VEKYLEATKFVKQLAARGGNVLFVGTKRAARELVATEAARAGMPFVDARWLGGMLTNF
                     KTVKTSIKRLKDMEVVVSEGGAERMIKKEGLLFQRELDKLNKSIGGIKDMTTLPEALF
                     VIDVGYHKIAVAEARTLGIPVVAVVDTNHSPDGIDYIIPGNDDSAKAIALYTKGIADA
                     VIEGRQQNLNGVVEELGEGQEEFVEVQDSQA"
     gene            2968..3792
                     /gene="map"
                     /locus_tag="VGC69_00485"
     CDS             2968..3792
                     /gene="map"
                     /locus_tag="VGC69_00485"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY0293788.1"
                     /translation="MEIVTDPIDLDKMRAACQDAARILDYLTPFVKPGITTGEIDRLC
                     KEYVLDELKVKSATAGYAPPGYPPYPGYICTSVNHVICHGIPGDKVLKNGDSINLDVT
                     IIKDGWFGDTSRMYAVGEQSILSRRLIDTTFECMWKGIQQVRPGATLGDIGHAIQKHG
                     EAQGFSIVREYCGHGVGRRFHQDPQVLHYGKPGTGLTLVPGMIFTIEPMVNAGRKEIR
                     NLADGWTVVTRDHSLSAQWEHSVLVTETGYEVLTLSPGVQQPPAFITEPLAIPAAA"
     gene            3817..6471
                     /locus_tag="VGC69_00490"
     CDS             3817..6471
                     /locus_tag="VGC69_00490"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY0293789.1"
                     /translation="MSFAELTQIKTRMQAARGAAIAGFRDHARPDTLLTELRRIVDRA
                     LRELLELHPLPEGAALAAVGGYGRGELYPHSDVDLLILLPDQAPRAALPPEGAGAAPG
                     RSGGGPAPSPADESAIETLVTSLWDLGLEPGHSVRTIEQCEQEAQADITVETALLESR
                     WLAGNRRLFKRFETAMDARLDARAFFLAKRVEMQQRHARHRDTPYALEPNCKESPGGL
                     RDLQVILWMARAANFGHSWNQIAQAGLLTPTEAHTLRNAEQVFKRLRIELHLLTNRRE
                     DRLLFDLQPGLAAVYGIADTATRRASELLMQRYYRAAGQVTQLNVILVQNIEERLFPR
                     PESEARAIDGDFRKLHERLDIVRDDAFERNPSLMLRAFLVMQQHVELTGMSARTMRAI
                     WHARGRIDAAFRKDPANHRLFIEILRQPHGIVHELRRMAMLDVLPRYLPVFKRIVGQM
                     QHDLFHVYTVDQHTLMVLRNVRRFTMPEHAQEYPLASQLIANLDKHWLLYVAALFHDI
                     AKGRGGDHSQLGAIEVRRFAKQHGLSAEDSELVEFLVRHHLLMSAVSQKRDLSDPAVI
                     EEFTRQVRDERHLTALYLLTVADIRGTSPKVWNAWKGKLLEDLYRLTLARLGGGHVDA
                     GTVLAQRQEEATRLIRLAGISDADRDQFWKQLDVAYFLRHEASEIAWHTRHLYRQHDP
                     ARPVVRARPTEQGEGLQVMVYTRDVPQLFVNTCGYFDSKSLSIQDARVHTTRHGWALD
                     SYIVLPPENGSDLRSLAALVEHELAERLASPGATQHNGPHVRQSRRSKAFPIVPQAEL
                     QPDEGSQSWRLTVTATDRPGLLHALAQVFAMNGVSLIMAKVMTLGDRVEDVFIVSGPV
                     LERPRSQMQFERGVLQALAGEEPQAQAA"
     gene            6509..7234
                     /locus_tag="VGC69_00495"
     CDS             6509..7234
                     /locus_tag="VGC69_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY0293790.1"
                     /translation="MAFYDYASAFGRSFFLAFATLLPILNPPACTPIFWTLTDGASHG
                     TRIDLSRRVAINAALLMIGSLIAGNILLSFFGISLAAVRVGGGLLVIYSGWRLVNSAD
                     ADTGRKAQMAEVFTPEMARARAFYPLTFPISVGPGCISAAITVGASLRSQHPSPLISA
                     IQYAGALPGLLLVALVLFLCLRFAESLMDKLGANGTAVFMRLSAFVLLCLGVQICWDG
                     AHDLLLDLIQDARHLPLATPSAS"
     gene            7267..7764
                     /locus_tag="VGC69_00500"
     CDS             7267..7764
                     /locus_tag="VGC69_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY0293791.1"
                     /translation="MTYTSHSRSNRPLGLIALFSFGALAVALISQYGFDMQPCAWCVF
                     QRLLYLAIGVLALIGLLGSLTGGRFLARLAALLGVLLSAGGIAAAWYQYSVASHMMSC
                     AQTFADRFMGATGLDAMVPWLFGIYATCADAMVKVLGVEYALWSLALFVLILLASLTA
                     LFKRR"
     gene            complement(7770..9293)
                     /gene="purF"
                     /locus_tag="VGC69_00505"
     CDS             complement(7770..9293)
                     /gene="purF"
                     /locus_tag="VGC69_00505"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0293792.1"
                     /translation="MCGIVGVIGRGPVNQLIYDSLLLLQHRGQDAAGIATSHGNHFNM
                     FKAHGLVRDVFRTRNMRSLPGTSGIGQVRYPTAGSSDSEEEAQPFYVNAPFGIMFAHN
                     GNLTNWRELRESLYRVDRRHINTNSDSEVLLNVLAHELQSAANGTSLDDNAIFRAVSM
                     VHKRARGAYAVVAQISGYGLLAFRDPFGIRPLCLGRLETEEGVEWMAASESVALEGSG
                     FTFVRDVQPGEAVFIDLDGHLTSRQCAENPQLVPCIFEYVYFARPDSLIDGVSVYDAR
                     LRMGEYLADKVARTMRLGDIDVVMPIPDSSRPAAMQLASRLNLNYREGLIKNRYVGRT
                     FIMPGQAMRKKSVRQKLNTIGMEFKGKNVLLIDDSIVRGTTSREIVDMARAAGANKVY
                     FASAAPPVRYPNVYGIDMPTQQELIATGRSDEEIARTIGVDGLIYQDLHDMQQSVRDL
                     NPELSRFEASCFNGEYVTGDITPEYLARLGQSRGGSSGDEETSGGLQFNMGYVANDL"
     gene            complement(9325..9813)
                     /locus_tag="VGC69_00510"
     CDS             complement(9325..9813)
                     /locus_tag="VGC69_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY0293793.1"
                     /translation="MTGFDFVVLAILGISCVLGLVRGLLKELLSLVAYVLAFVAAIWW
                     GPVVYGWLAPYIQTTLLRMGVAYAAVFIGALLAVGLVNMTLSALVRVTGLSPADHGLG
                     GLFGLARGLVIILALVVVGGYTPLPKEPWWQNAMFSQSAVQAVKHIKSWLPPSLATWL
                     PY"
     gene            complement(9810..10676)
                     /locus_tag="VGC69_00515"
     CDS             complement(9810..10676)
                     /locus_tag="VGC69_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0293794.1"
                     /translation="MGLFTRKDSAAARKGNNGVRPRPSVSGEAQAAELRARARRRLAG
                     AVILVLAAVIILPMVLDSRPIPVASDIPVRIPEKDAPYQPTVSEPQAQPPVQQPAQAA
                     PAAPVPAAPPAPLASNPAPSTAAPAAAGHVAPPAVLAPVPVPEAPAAVPRPAAKPEAK
                     PAENKPEPKPAPAARTDDGSVALALLEGRKPAGAAPAKTTPGGGNASGNAGGNYVLQI
                     AAYGTQADAQARNTQLHQSGITNAYVEPSNAGGKSQYRLRVGPFPSREAAQAAQARLR
                     ALGYDNGFIAAQ"
     gene            complement(10710..>11021)
                     /locus_tag="VGC69_00520"
     CDS             complement(10710..>11021)
                     /locus_tag="VGC69_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY0293795.1"
                     /translation="AVFGMLADKDLAGVVAKLGSRIDHWFCTGLPGPRGTSGEALAEQ
                     VRAALPNAPADGEAPGVQAFPDPVAAYAAARERAGENDRIVVFGSFLTVSAVLQALGR
                     V"
BASE COUNT         1760 a         3796 c         3577 g         1888 t
ORIGIN      
        1 agatttcctt gttttatacg tgtaccagcg tgccttcgtc ttcaccggcc acagcgcgct
       61 tgagcgcgcc cggcttgttg atcgaaaaca ccttgatggg cagtttctgg tcgcggcaca
      121 gcgcgaacgc agtggcgtcc atcacttcga ggcggcgcac gatggcttcg tcgaagctga
      181 tgcgcgcgta gcgcgtggcg gtggggtcct tgttgggatc ggcgctgtag atgccgtcga
      241 ccttggtggc cttgagcacg atctcggcgc cgatctcggc gccgcgcagc gccgcggcgg
      301 tatccgtggt gaagaagggg ttgcccgtgc cggccgcgaa gatcacgacc ttgccttcct
      361 cgagatagcg cagcgccttg ggacggatgt agggctcgac cacctgctcg atgttcaggg
      421 ccgattgcac gcgggcgtcg acaccctggt gcttgagcgc gtcctgcagg gccagcgaat
      481 tcatgatggt ggccatcatg cccatgtagt cggccgtggc gcggtccatg ccctgggcac
      541 ccggcgccac gccgcggaag atattgcccc cgccgatgac gatggccagc tcgataccgg
      601 tggccgcgac ctcggcgatt tcctgggtca tgcggatgat ggtgccgcga ttgatgccgt
      661 aggcatcctc gcccatcaag gcctcgccgg aaagcttgag tagaacccgt ttgtacgatc
      721 tgctgctggt catgggcatg cccctgggaa ctgttccgac gaaagtgtaa gacaagaaag
      781 agaaatgaaa tgccccacgc tgcgccttcg gcttgctgcc cccccgaggg ggccgttttc
      841 gccctggggc ggcaaaaagg ccggagccgg gtctttatcg cagaccccgc gccggcctcg
      901 gcatcgccag gagatcaggc ctggccggca gcggcggcag ccacttcggc ggcgaaatcg
      961 gtgaccttct tctcgatgcc ctcgcccacc acgaacagca cgaacttgct gatcgaggcg
     1021 ttcttttcct tgagcatctg ctcgatggtc tgcttgtcgt tcttgacgaa gggctgcgac
     1081 agcagcgtga cttccttcag gaacttggcg atcgagccgt cgaccatctt ggcgacgatt
     1141 tcggcgggct tgcccgattc ggcggccttc tgctcggcga ccgagcgttc ggtggcgatg
     1201 tcggcggcgg gcacgccgtc cgcgctcaag gccttgggct tggtggcggc gatgtgcatg
     1261 gccaggtcct tgcccacttc gtcggcgccg gtgaactcga ccagcacgcc aatgcgcccg
     1321 ccgtgcacgt agctggccag cgcattgggc gtctggatgc gctcgaagcg gcggatggac
     1381 atgttttcgc cgatcttgcc gatcagggcg gtgcgggtgg tctcgaccgt gccttcgcca
     1441 tagggcagcg ccgacagggc gtccacattg gcggggtttt tctcggcgac cagcttggcc
     1501 aggccgttga cgaaagccac gaagtcattg ttcttgccca cgaagtcggt ttcgcagttg
     1561 acttcgatca ccgcgccctg cttgccgtcg gcggagatga acaggccgat caggccttcg
     1621 gcggtgacgc gggcggcggc cttgctggcc ttgttgccca gcttgacgcg caggatttcc
     1681 tcggcgcggg ccaggtcgcc ttcggcctcg gtcagggcct tcttgcattc catcatgggg
     1741 gcgtcggtct tttcgcgcag ttccttgacc agggaggcgg taatttcagc catgtttgct
     1801 ccaaatttgg taagacatgc cccggtcggg ccggggcaag aaatggggtt gctgggacgg
     1861 agccgtcggc ggggttgccc ccgctcggca cccgtcccgg cggcccgcct ggacaggctg
     1921 gccgccggac atgacaggca tcaggcctgg ctgtcctgca cttcgacgaa ctcttcctgg
     1981 ccttcgccca gctcttcaac gacgccgttc aggttttgct ggcggccttc gatgacggcg
     2041 tcggcgatgc ccttggtgta cagcgcgatg gccttggccg agtcgtcgtt gccggggatg
     2101 atgtagtcga tgccgtcggg cgaatggttg gtatcgacca cggccaccac cgggatgccc
     2161 agcgtgcggg cttcagccac ggcgatcttg tggtagccca cgtcgatcac gaacagggcc
     2221 tcgggcagcg tggtcatgtc cttgatgccg ccgatcgact tgttcagctt gtcgagttcg
     2281 cgctggaaca gcaggccttc cttcttgatc atgcgctcgg cgccgccctc ggacacgacg
     2341 acttccatgt ccttcaggcg cttgatcgag gtcttgaccg tcttgaagtt ggtcagcatg
     2401 ccgccgagcc agcgagcgtc cacgaaaggc atgccggcgc gggcggcctc ggtggcaacc
     2461 agctcgcggg cggcgcgctt ggtgccgacg aagagcacgt tgccgccgcg ggcagccagc
     2521 tgcttcacga acttggtggc ttcgaggtac ttttcaaccg tcttttcgag gttgacgatg
     2581 tggattttgt tacggctgcc gaagatgtag ggagccatct tcgggttcca gtaacgggtc
     2641 tggtggccga agtggacgcc ggcttcaagc atttcgcgca tcagggacat aatattctcc
     2701 tagggtttgg actggcgctc ttgccagcac ccgcaaggct tgactcaagc cgcgggcacc
     2761 ctggttggca aggcgcgcga tttggcagcg cacgggcaaa aaagccgaac cgctgcctgc
     2821 ttccgcatca ctcccgggat gggttttctc ggaaagaaat gtcggaaagc ctataattct
     2881 actatttttc agggcaagcc ctcaagtagc ctgcccactg ccccggaaaa ccgggacaaa
     2941 accaccgaat tcacgctgga caacgccatg gaaatcgtta ccgaccccat cgacctggac
     3001 aagatgcgcg ccgcctgcca ggacgccgcc cgcatcctgg actacctcac tcccttcgtc
     3061 aaaccgggga tcaccaccgg cgagatcgac cgcctgtgca aggaatacgt cctcgacgaa
     3121 ctcaaggtga agtcggccac cgcgggctac gcccccccgg gctacccccc ctaccccggc
     3181 tacatctgca cctcggtcaa ccacgtgatc tgccatggca tcccgggcga caaagtcctc
     3241 aaaaacgggg actccatcaa tctggacgtc accatcatca aggacggctg gttcggcgac
     3301 accagccgga tgtatgccgt gggcgagcag tccatcctgt cgcgccgcct gatcgacacc
     3361 acgttcgaat gcatgtggaa aggcatccag caggtgcgcc ccggcgccac gctgggcgac
     3421 atcggccatg ccatccagaa gcacggcgaa gcccagggct tctcgatcgt gcgtgaatac
     3481 tgcggccacg gcgtgggccg ccgcttccac caggacccgc aggtgctgca ctacggcaag
     3541 ccgggcaccg gcctcacgct ggtgcccggc atgatcttca ccatcgagcc catggtcaac
     3601 gccggccgca aggagatccg caacctggcc gacggctgga cggtggtcac ccgcgaccac
     3661 agcctgtccg cgcagtggga gcacagcgtg ctggttaccg agaccggcta cgaagtgctg
     3721 accctttcgc ctggcgtgca gcagcccccg gccttcatca ccgagccgct ggccatcccg
     3781 gccgccgcct gaccccacat ccgacctatt cgctgcatga gttttgccga gctgacccag
     3841 atcaagaccc ggatgcaggc ggcgcgcggc gccgcgattg ccggcttccg cgatcacgcg
     3901 cgccccgaca cgctgctcac cgaactgcgc cgcatcgtcg accgggccct gcgcgagctg
     3961 ctcgaactgc accccctgcc cgaaggcgcc gcgctggcgg ccgtgggcgg ctatggccgc
     4021 ggcgaactgt acccgcactc cgacgtcgac ctgctcatcc tgctgccgga tcaggccccc
     4081 cgcgctgcgc tgccccccga aggggccgga gccgccccgg ggcggtccgg cggcggcccc
     4141 gcgccgtcgc ccgccgacga aagcgccatc gagacgctcg tcacctcgct ctgggacctg
     4201 ggcctggagc ccggccacag cgtgcgcacc atcgagcagt gcgaacagga agcccaggcc
     4261 gacatcacgg tggaaacggc cctgctcgaa tcgcgctggc tggccggcaa ccggcgcctc
     4321 ttcaagcgct tcgagaccgc catggatgcc cgcctggacg cgcgcgcctt cttcctggcc
     4381 aagcgcgtgg agatgcagca gcgccatgcg cgccatcgcg acacgcccta tgcgctggaa
     4441 cccaactgca aggagtcgcc cggcggcctg cgcgacctgc aggtgatcct gtggatggcg
     4501 cgcgcggcca acttcggcca cagctggaac cagatcgcgc aggccggcct gctgacgccg
     4561 accgaggcgc acacgctgcg caacgccgaa caggtcttca agcgcctgcg catcgagctg
     4621 cacctgctca ccaaccgccg cgaagaccgg ctgctgttcg acctgcagcc cggcctggcc
     4681 gccgtgtacg gcatcgccga cacggccacg cgccgcgcca gcgaactgct catgcagcgc
     4741 tactaccgcg ccgcgggaca ggtgacgcag ctcaacgtga tcctggtgca gaacatcgaa
     4801 gagcgcctct ttccccgccc ggaaagcgag gcgcgcgcca tcgacggcga cttccgcaag
     4861 ctgcacgagc gcctggacat cgtccgcgac gacgccttcg agcgcaatcc cagcctgatg
     4921 ctgcgcgcct tcctggtgat gcagcagcat gtggagctga ccggcatgtc ggcgcgcacc
     4981 atgcgcgcca tctggcacgc gcgcggccgc atcgacgcgg ccttccgcaa ggacccggcc
     5041 aaccaccgcc tgttcatcga gatcctgcgc cagccgcacg gcatcgtgca cgagctgcgc
     5101 cgcatggcca tgctcgacgt gctgccgcgc tacctgccgg tgttcaagcg catcgtgggc
     5161 cagatgcagc acgacctgtt ccatgtgtac acggtcgacc agcacacgct gatggtgctg
     5221 cgcaatgtgc gccgcttcac catgcccgag cacgcgcagg aatacccgct ggcgagccag
     5281 ctgatcgcca acctggacaa gcactggctg ctgtacgtgg cggcgctctt tcacgacatc
     5341 gccaagggcc gcggcggcga ccactcgcag ctgggcgcca tcgaagtgcg ccgcttcgcc
     5401 aagcagcacg gcctgagcgc cgaggacagc gaactggtgg agttcctggt gcgccatcac
     5461 ctgctgatgt cggccgtgtc gcaaaagcgc gacctctccg acccggccgt catcgaagaa
     5521 ttcacccggc aggtgcgcga cgaacggcac ctcaccgcgc tctacctgct gaccgtggcc
     5581 gacatccgcg gcaccagccc caaggtgtgg aacgcctgga agggcaagct gctcgaggac
     5641 ctctaccggc tgaccctggc ccgcctgggc ggcggccatg tcgacgccgg caccgtcctg
     5701 gcccagcgcc aggaagaagc cacgcgcctg atccgcctgg ccggcatttc cgacgccgac
     5761 cgcgaccagt tctggaagca gctcgacgtg gcgtacttcc tgcgccacga ggcttcggaa
     5821 atcgcctggc acacgcgcca cctgtaccgc cagcacgatc ccgccaggcc ggtggtgcgc
     5881 gcccgcccca ccgagcaagg cgaaggcctg caggtcatgg tctatacccg cgacgtgccg
     5941 cagctcttcg tcaatacctg cggatatttc gacagcaagt cgctcagcat ccaggatgcc
     6001 cgcgtgcaca ccacccggca cggctgggcg ctggacagct atatcgtcct gccccccgag
     6061 aacggcagcg acctgcgctc gctggccgcc ctggtcgagc acgaactggc cgaaaggctg
     6121 gccagcccgg gcgcaacgca gcacaacggc ccccatgtcc ggcaatcgcg ccggtccaag
     6181 gccttcccca tcgtgcccca ggccgaactg cagcccgacg aaggcagcca gtcgtggcgg
     6241 ctgacggtga ccgccaccga ccggcccggc ctgctgcatg cgctggcgca ggtcttcgcc
     6301 atgaacgggg tcagcctcat catggccaag gtcatgacgc tgggcgatcg cgtggaagac
     6361 gtcttcatcg tgtcgggccc cgtgctcgag cgcccgcgca gccagatgca gttcgagcgc
     6421 ggcgtgctgc aggccctggc cggcgaagag ccccaggcgc aggcggcctg agtacaatcg
     6481 cggaaatctt tttcccggcg ccgcctgcat ggccttctac gattacgcct ccgcgttcgg
     6541 acgcagcttt ttcctggcct tcgccaccct gctgcctatc ctgaacccgc ccgcgtgcac
     6601 gcccattttc tggacgctga ccgacggcgc ctcgcacggc acgcgcatcg acctgtcgcg
     6661 ccgcgtggcc atcaatgccg cgctgctgat gatcggctcg ctgatcgcgg gcaatatcct
     6721 gctcagcttt ttcggcattt cgctggcggc cgtgcgcgtg ggcggcggcc tgctggtgat
     6781 ctacagcggc tggcggctgg tgaactcggc cgacgccgac accggccgca aggcccagat
     6841 ggccgaggtc ttcacccccg aaatggcgcg ggcgcgcgcc ttctatccgc tgacctttcc
     6901 catcagcgtg ggcccgggct gcatttccgc cgcgatcacc gtgggcgcct cgctgcgcag
     6961 ccagcacccc tcgccgctca tcagcgccat ccagtacgcc ggcgccctgc ccggcctgct
     7021 gctggtggcg ctggtgctgt ttctctgcct gcgctttgcc gaatcgctca tggacaagct
     7081 gggcgccaac ggcacggcgg tcttcatgcg gctgtcggct ttcgtgctgc tgtgcctggg
     7141 cgtccagatc tgctgggacg gcgcccacga cctgctgctg gacctgatcc aggatgcccg
     7201 gcaccttccc ctggctactc cctcggcctc gtaacccggc cgcctaccct ttcagagcat
     7261 gcatccatga cctacacgtc tcactcccgc agcaaccgcc cgctgggcct gatcgccctg
     7321 ttttccttcg gcgccctggc cgtggcgctg atctcgcaat acggattcga catgcagccc
     7381 tgcgcctggt gcgtgttcca gcgcctgctg tatctggcga tcggcgtgct ggccctgatc
     7441 ggcctgcttg gtagtttgac tggcggccgg ttcctggccc gcttagcggc cctgctgggc
     7501 gtgctgctgt cggccggcgg cattgccgcc gcctggtatc agtacagcgt ggcctcgcac
     7561 atgatgtcgt gcgcgcagac ctttgccgac cggttcatgg gcgccacggg gctcgacgcc
     7621 atggtgccct ggctgttcgg catctacgcg acctgcgccg acgccatggt gaaggtgctg
     7681 ggcgtggaat acgccctgtg gagcctggcg ctgttcgtgc tgatcctgct ggcgtcgttg
     7741 acggcgctgt tcaagcgccg ctgatcgcat cagaggtcgt tggccacgta gcccatattg
     7801 aactgcaggc cgcccgaagt ctcttcatcg ccggagctgc cgccgcgcga ctggcccagc
     7861 cgggccaggt attcgggcgt gatgtcgccg gtgacgtact cgccgttgaa gcacgaggcc
     7921 tcgaagcgcg acagctcggg gttgagatcg cgcaccgact gctgcatgtc gtgcaggtcc
     7981 tggtagatca ggccgtccac gccgatggtg cgggcgatct cttcgtcgct gcggccggtg
     8041 gcgatgagtt cctgctgcgt gggcatgtcg atgccgtaca cattgggata gcgcaccggc
     8101 ggcgcggccg aggcgaagta caccttgttc gcgccggcgg cgcgcgccat gtcgacgatc
     8161 tcgcggctgg tggtgccgcg cacgatggag tcgtcgatca gcagcacgtt cttgcccttg
     8221 aactccatgc cgatggtgtt gagcttctgg cgcacggact tcttgcgcat ggcctggccc
     8281 ggcatgatga aggtgcggcc cacatagcgg ttcttgatga ggccttcgcg gtagttcagg
     8341 ttcaggcgcg aggccagctg catggcggcc gggcgcgatg aatcgggaat cggcatgacc
     8401 acgtcgatgt cgcccaggcg catggtgcgc gccaccttgt cggccaggta ctcgcccatg
     8461 cgcaggcggg cgtcatacac cgacacgccg tcgatcagcg aatcggggcg ggcaaagtac
     8521 acgtattcga aaatacaggg caccagctgc ggattctcgg cgcactggcg gctggtcagg
     8581 tggccgtcca ggtcgatgaa caccgcctcg cccggctgca cgtcgcgcac gaaggtaaag
     8641 ccgctgcctt ccagcgccac cgattcggag gccgccatcc actccacgcc ctcttcggtt
     8701 tccagcctgc cgaggcacag cgggcggatg ccgaaggggt cgcggaaggc cagcaggccg
     8761 tagcccgaga tctgcgccac cacggcgtag gcgccgcgcg cgcgcttgtg caccatggac
     8821 acggcgcgga agatcgcgtt gtcgtccagc gaggtgccat tggccgccga ctgcagctcg
     8881 tgcgccagca cattgagcag cacctcggag tcggagttgg tattgatgtg gcggcggtcg
     8941 acgcggtaga gcgactcgcg cagttcgcgc cagttggtca ggttgccgtt gtgggcgaac
     9001 atgatgccga agggcgcgtt cacgtagaag ggctgggcct cttcctcgct gtcgctggaa
     9061 ccggccgtgg gatagcgcac ctggccgatg ccgctggtgc cgggcagcga acgcatattg
     9121 cgggtgcgga acacgtcgcg caccaggccg tgcgccttga acatgttgaa gtgattgccg
     9181 tgcgacgtgg caatgcccgc cgcgtcctgg ccgcgatgct gcagcagcag caggctgtca
     9241 tagatcaact ggttgaccgg cccgcgcccg atgaccccga cgattccaca catggtgagt
     9301 tccttgattg aagcctgata ctgctcaata cggcagccac gtcgccagcg acggcggcaa
     9361 ccacgacttg atatgcttta ccgcctggac ggccgactgc gaaaacatgg cgttctgcca
     9421 ccagggctcc ttgggcagcg gggtataacc gcccaccacc accagcgcca ggatgatgac
     9481 caggccccgg gccagtccga acaggccgcc caggccgtga tcggcgggcg acaggcccgt
     9541 cacgcgaacc agcgccgaca gggtcatgtt gaccaggccc acggccagca aggcgccgat
     9601 gaataccgcc gcataggcca cgcccatgcg caacagcgtc gtttgtatat agggagccaa
     9661 ccagccatac acgacgggcc cccaccatat cgccgcgacg aaagccagca cgtacgccac
     9721 cagcgacagc aattccttca gcaagccgcg caccagcccc agcacgcagg agatgcccag
     9781 gatcgccagc acgacaaagt caaacccggt cactgtgcag cgatgaagcc gttgtcataa
     9841 cccagcgccc gcaggcgcgc ctgtgccgcc tgagccgcct cgcgcgaagg gaacggcccc
     9901 acgcgcaacc ggtactgcga cttgccgccc gcattcgatg gctcgacata ggcgttggtg
     9961 atgcccgact ggtgcagctg cgtattgcgc gcctgggcat cggcctgcgt gccgtacgcc
    10021 gcgatctgca gcacgtagtt gccacccgca ttgccgctgg cgttgccgcc ccccggcgtc
    10081 gtcttggccg gcgcggcgcc ggccggcttg cggccttcga gcagggccag ggcgaccgaa
    10141 ccgtcatcgg tacgcgccgc gggcgccggc ttgggttcgg gcttgttctc ggcgggcttg
    10201 gcttccggct tggcggcagg acgcggcacg gctgccggcg cctcgggcac gggcaccggc
    10261 gccaggactg ctggcggcgc gacgtggccc gctgccgccg gggcagcggt ggacggcgcg
    10321 ggattggagg caagcggcgc aggcggcgcg gccggcacgg gcgcggcggg cgctgcctgg
    10381 gcaggctgtt gaaccggagg ctgggcctgc ggctcggaga cggtgggctg gtaaggcgca
    10441 tccttctcgg ggatgcgcac cgggatgtcg ctggcgaccg ggatgggccg ggaatcgagc
    10501 accatgggca ggatgatcac ggcggccagc accaggatca ccgcaccggc caagcgccgg
    10561 cgggcgcggg cgcgcaattc ggccgcctgg gcctcgccgg acacggaggg gcgtggacgc
    10621 acgccattgt tgcctttcct ggccgcggca gaatctttgc gtgtgaataa gcccattatg
    10681 gcgcccgatg tcgttccatc attgcccgct caaacccgac ccagtgcctg caataccgcc
    10741 gacacggtca ggaaggaccc gaacaccacg attctatcat tctcccccgc cctttcccgg
    10801 gcggcggcat aggccgcgac cgggtccgga aaggcttgca cgcccggcgc ctcgccatcg
    10861 gcgggcgcgt tcggcaaggc ggcccgcact tgttcggcca gcgcctcgcc gctcgtgccg
    10921 cgcggccccg gcaggcccgt gcagaaccag tggtcgatgc ggctgcccag cttggcaacc
    10981 acgccggcga gatccttgtc ggccagcatg ccgaacaccg c
//
LOCUS       DASWPO010000014         6853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_895659, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000014 DASWPO010000000
VERSION     DASWPO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895659"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VGC69_00525"
     CDS             complement(<1..698)
                     /locus_tag="VGC69_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0293796.1"
                     /translation="MKVVYSDRHQGHDPQYFIVRGRMKRSNEQPERALLLLEAARAQG
                     HDIVMAEDFGAAPRAAIHTPEYLHFLETVWEGWQQLEDASDEVLPNVHPFPGQPFTYP
                     DSLVGRAGYHMGDMACSIGPHTWEAAIWSAHAATHAADLVLGGERAVYALCRPPGHHA
                     YADRANGFTYLNNAAIAAQYLRRRHERVAILDVDVHHGNGTQGIFWRRKDVLTVSLHG
                     DPHFCTPFFTGHAH"
     gene            complement(695..1414)
                     /locus_tag="VGC69_00530"
     CDS             complement(695..1414)
                     /locus_tag="VGC69_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0293797.1"
                     /translation="MTRSAAADAFTSIRSPDLVDIAEAQIQAAILNGQFAPGERIIEA
                     ELARRMGISRAPVREAARRLESAGLLTSRPRHGFFVKTLSVREVDDLYQVRIDLELLG
                     VRLACAQASDGQLARLVTMVDHMVEQSSRLNRAERVALDLGFHAYIAEISGNLYLQRL
                     FDNMQAEVRMFQSLNEPGYEDPHFVAESHRPIVKAFIRRDAEATQAALRFHLEAAHEQ
                     TRVCVARAGLNPSGPLQGTQT"
     gene            complement(1417..2376)
                     /locus_tag="VGC69_00535"
     CDS             complement(1417..2376)
                     /locus_tag="VGC69_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY0293798.1"
                     /translation="MPDPTIALLSSQIDTDYLLEAFRAEFPGARLVLGGRSQAEADLG
                     ELGAIDLVVCWMPQHGLMHRLPNLKMVQSVGAGIDHITADPLLPGVPICRIIDPEMAS
                     GMAAYVCWAVTHRQCSIPAYLESQRRGAWEEQPIVPARRHRVGIAGLGTLGLQCARAL
                     AAIGYAVSGWSRTPKPQLAEGIQAWHGDDQLDDFLAQCDTLVCLLPLTGRTRGILDAR
                     LFSKLPRGAHLINVGRGDHLVERDLLAALDAGQLGAATLDAFSAEPLPADHPFWTHPR
                     VTVTPHIATRTPPSTIARQTRANYEALLAGRTPAGAMDRARGY"
     gene            complement(2369..4045)
                     /locus_tag="VGC69_00540"
     CDS             complement(2369..4045)
                     /locus_tag="VGC69_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0293799.1"
                     /translation="MNARASGQAAQLSGPAGTAPVLDVRGLSIKLPDGGDRRLAVDSV
                     SFSLPPGRTLCIVGESGSGKSMIANAVMGLLPRPAVAPVAGEILFGGQDLLKLDEAGM
                     RELRGRRIGMIFQEPMTALNPVMRIGTQLAEVFDAHLTLPAAEKRRRIIAALTDVGLP
                     DPHLLVDSYPFRLSGGQRQRVMIACALLLEPHLLIADEPTTALDVTTQAQILKLIRDL
                     QQRRGTAVLFITHDFGVVAEIADHVIVMQTGNMVEAGPASQVLQQPRHAYTRKLLAAI
                     PSGRIRQPPDQRELQRALEVQDLDKTYASGGGLFRKGRRVHAADGVNIDLRRGETLGL
                     VGESGSGKSTVGRCLVGLTPYDGGRILFKGQIMPSGPGFRTLAQGKIQMVFQDPYASL
                     NPRHRVGAAIAAGPIAQGVAPDEARARALRLLELVGLGKEAAARFPHEFSGGQRQRIG
                     IARALAMEPELLVADEPVSALDVSVQAQVLELFAQVRDEFKLSMVFITHDLRVAGQMC
                     DHIAVMQRGRIVEYGPTAEVLANPQHAYTRSLIDAIPKLARPAGKEAADA"
     gene            complement(4042..4884)
                     /locus_tag="VGC69_00545"
     CDS             complement(4042..4884)
                     /locus_tag="VGC69_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0293800.1"
                     /translation="MNHSFWRRFRRNRGATLGLAFLIFIVLLAAFGPFFAANDPWDMV
                     SSPFVPPMGEPHLLMGTDTLGRDVLSGVIYGARVSLLIGVISTVVSLLIGVSLGAIAG
                     YFGGWVDASLMRFTEMFQAVPSFALAIVLVAIFEPSIYSIVAAIAVVSWQPVARLVRG
                     EFLTLRQRDFVSAALLAGESTPRVILTQILPNALSPIVVMASLMIATAILLESSLSFM
                     GLGDPNMMSWGYMIGAARTVLRNAWWMAAFPGLAIVLTVLALNLVGEGLNDGLNASAG
                     GRGR"
     gene            complement(4915..5889)
                     /locus_tag="VGC69_00550"
     CDS             complement(4915..5889)
                     /locus_tag="VGC69_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0293801.1"
                     /translation="MKRFQFMLTRLAQGIVAILAIATVNFLLVHAAPGDPVSVMAGEA
                     GTADPQFVAQLKRQFGLDQPIGVQLGHYLDHIVHLDLGYSYRQQQPVLDLILHRLPAT
                     LLLTGSAFVLSLVFGVALGAWASRKAGSWVDSAITVVALVFYATPLYWLAMMAVLLFS
                     IKLDWLPGFGYFTVGAGLSGLAQAWDIFEHLVLPSLTLALFYMAVYARMTRASMLEVA
                     QMDFVKTARAKGLRPPRIQRAHILRNALLPVVTLAGIQAGGMIGGAVLTETVFAWPGI
                     GRLMFDALLQRDYTLLLGCFLVTSAMAVLLNLVTDLVYTLVDPRIELS"
     gene            complement(5971..>6853)
                     /locus_tag="VGC69_00555"
     CDS             complement(5971..>6853)
                     /locus_tag="VGC69_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0293802.1"
                     /translation="IPDAASRAAAFESGKVDVVPGGAVEFFDVARLAKLPGAAVTTKG
                     WEFFAPHSWLCLNNRNKPLDNVKVRQAIMTAIDHESIAKIAWQGYAKPAIGPFNSHIK
                     YYTDKVAKYPHDIAKAKQLLKDGGYAGQTLRLLPLPYGESWQRQAEIVRQNLTQAGVK
                     VDMVATDVAGWNQRLSQWDYDLAFNYVYQYGDPALGVARNYTASNIAKGSPFNNVEGY
                     ANPKVDELFDSAGREVDTAKRTALYEEVQKILVDDVPVAWLHEINFPTLYRTKVYNLI
                     SSGIGLNDGLGYAKLTA"
BASE COUNT         1038 a         2165 c         2589 g         1061 t
ORIGIN      
        1 tcgtgcgcat ggccggtgaa gaagggcgtg cagaagtgcg ggtcgccgtg cagcgagacg
       61 gtcagcacgt ccttgcggcg ccagaagatg ccctgggtgc cgttgccatg gtgcacgtcg
      121 acgtccagga tggcgacgcg ctcgtgccgg cgccgcaggt attgcgcggc gatggcggcg
      181 ttgttgaggt aggtgaagcc gttggcgcgg tcggcgtagg catggtggcc gggcggacgg
      241 cacagggcgt agacggcgcg ctcgccgccc agcaccaggt cggccgcgtg ggtggcggcg
      301 tgggccgacc agatcgcggc ttcccaggtg tgcgggccga tcgagcaggc catgtcgccc
      361 atgtggtagc cggcgcggcc caccaggctg tcgggatagg taaagggctg gccggggaag
      421 ggatgcacgt tgggcagcac ttcgtcggag gcgtcctcga gctgctgcca gccttcccag
      481 acggtttcca gaaagtgcag gtattccggg gtgtggatgg cggcgcgcgg cgcggcgccg
      541 aaatcctcgg ccatgacgat gtcgtggcct tgcgcgcgcg ccgcctcgag cagcagcagg
      601 gcgcgctcgg gctgctcgtt gctgcgcttc atgcggccgc ggacgatgaa gtactgcggg
      661 tcgtgtccct ggtgccgatc gctatagacg actttcatgt ctgggttcct tggaggggtc
      721 cggatggatt caagccggcc cgggccacgc agacgcgggt ctgttcgtgc gcggcttcca
      781 ggtggaagcg cagcgcggct tgcgtggcct cggcatcgcg ccggatgaat gccttgacga
      841 tgggccggtg gctttcggcg acgaagtgcg ggtcttcgta gccgggttcg ttcagcgact
      901 ggaacatgcg cacctcggcc tgcatgttgt cgaacagccg ctgcaggtac aggttgccgg
      961 agatttccgc gatgtaggca tgaaagccca ggtccagcgc cacccgttcg gcgcggttca
     1021 ggcggctgct ttgttcgacc atgtggtcga ccatggtcac cagccgggcg agctgcccgt
     1081 cgctggcctg cgcgcaggcc agccggacgc ccagcagttc gaggtcgatg cgcacctggt
     1141 agaggtcgtc cacttcgcgc acggacagcg tcttgacgaa gaagccgtgg cgcggccgcg
     1201 aggtcagcag gcccgcgctt tccaggcggc gggccgcctc gcgcaccggc gcgcggctga
     1261 tgcccatgcg gcgcgccagc tcggcctcga tgatgcgctc gccgggcgcg aactggccgt
     1321 tgaggatggc ggcctggatc tgcgcttcgg cgatgtcgac caggtcgggc gaccggatcg
     1381 aggtgaaggc gtcggcggcg gcggagcggg tcatgttcag taccctcgtg cgcggtccat
     1441 cgcgccggcg ggcgtgcggc cggccagcag ggcctcgtag ttggcgcggg tctgccgggc
     1501 gatggtcgac ggcggggtgc gcgtggcgat gtgcggcgtc accgtgacgc gcgggtgcgt
     1561 ccagaaggga tggtccgccg gcaggggttc ggccgagaag gcgtcgagcg tggccgcgcc
     1621 cagctggccg gcgtcgagcg cggccagcag gtcgcgttcg accaggtggt cgccgcgtcc
     1681 cacgttgatc aggtgggcgc cgcgcggcag cttgctgaag agccgggcat cgaggatgcc
     1741 gcgcgtgcgg ccggtgagcg gcagcaggca gaccagggtg tcgcattgcg cgaggaagtc
     1801 gtcgagctga tcgtcgccgt gccaggcctg gatgccttcg gcaagctgcg gcttgggcgt
     1861 gcggctccag cccgacacgg cgtagccgat ggccgccagc gcgcgggcgc attgcaggcc
     1921 cagcgtgccc aggccggcga tccccacgcg gtgccggcgg gccggcacga tgggctgttc
     1981 ttcccaggcg ccgcggcgct ggctttccag gtaggcgggg atgctgcatt ggcggtgcgt
     2041 gacggcccag cacacatagg ccgccatgcc gctggccatc tcggggtcga tgatgcggca
     2101 gatgggcacg ccgggcagga gcgggtcggc ggtgatgtgg tcgatgccgg cgcccaccga
     2161 ctgcaccatt ttgagattgg gcaggcggtg catgagcccg tgctgcggca tccagcacac
     2221 cacgaggtcg atggcgccga gctcgcccag gtccgcttcg gcctggctgc ggccgcccag
     2281 caccagccgg gcgccgggga attcggcgcg gaaggcctcc agcaggtagt ccgtgtcgat
     2341 ctggctgctg agcagggcga tggtggggtc aggcatcggc ggcctccttg ccggcggggc
     2401 gcgccagttt gggaatggcg tcgatcaggg aacgggtata ggcgtgttgc gggttggcca
     2461 ggacctcggc ggtcgggccg tattcgacga tgcggccgcg ctgcatgacg gcgatgtggt
     2521 cgcacatctg tccggccacg cgcaggtcgt gcgtgatgaa caccatggac agcttgaatt
     2581 catcgcgcac ctgggcgaac agttcgagca cctgggcctg gaccgagacg tccagcgccg
     2641 acacgggctc gtcggccacc agcagttccg gctccatcgc cagggcgcgg gcgatgccga
     2701 tgcgctggcg ctggccgccg gagaactcat gcggaaagcg cgcggcggcc tctttgccca
     2761 ggccgaccag ctcgagcagg cgcaaggcgc gcgcgcgcgc ctcgtccggc gcgacgcctt
     2821 gcgcgatggg ccccgcggcg atggcggcgc cgacgcggtg gcgtgggttg agcgaggcgt
     2881 aggggtcctg gaacaccatc tggatcttgc cttgcgccag ggtccggaag cccgggccgg
     2941 acggcatgat ctgccccttg aacaggatgc ggccgccgtc gtagggcgtc aggccgacca
     3001 ggcagcggcc gaccgtggac ttgcccgagc ccgattcgcc caccaggccc agcgtttcgc
     3061 cgcgccgcag gtcgatattg acgccgtcgg ccgcgtgcac ccggcggccc ttgcggaaca
     3121 ggccgccgcc ggaggcgtag gtcttgtcca ggtcctgcac ctcgagcgcg cgctgcagtt
     3181 cgcgctggtc gggcggctgg cgtatccggc ccgaggggat ggccgccagc agcttgcggg
     3241 tgtaggcgtg ccgcggctgc tgcagcacct gcgaggccgg gccggcctcg accatattgc
     3301 ccgtctgcat cacgatgacg tggtcggcga tctcggccac gacgccgaag tcgtgcgtga
     3361 tgaagagcac ggcggtgccg cggcgctgct gcaggtcgcg gatcagcttg aggatctggg
     3421 cctgggtggt gacgtccagc gcggtggtgg gttcgtcggc gatcagcagg tgcggctcca
     3481 gcagcagggc gcaggcgatc atcacgcgct ggcgctggcc gcccgagagc cggaagggat
     3541 agctgtcgac cagcaggtgc gggtcgggaa ggccgacatc ggtcagcgcc gcgatgatgc
     3601 gccggcgctt ctcggcggcg ggcagcgtca ggtgcgcgtc gaacacctcg gccagctggg
     3661 tgccgatgcg catgacgggg ttgagcgccg tcatgggctc ctggaagatc atgccgatgc
     3721 gccggccgcg cagttcgcgc atgccggcct cgtcgagctt gagcaggtcc tggccgccga
     3781 acaggatttc gcccgccacg ggcgccaccg cggggcgggg cagcagcccc atgacggcgt
     3841 tggcgatcat ggacttgccg gagccggatt cgcccacgat gcacagcgtg cgtcccggcg
     3901 gcaggctgaa gctgaccgag tccacggcca gccgccggtc gccgccgtcg ggcagcttga
     3961 tgctcaggcc gcggacgtcc agcacggggg cggtcccggc ggggccggac aactgggcgg
     4021 cttggccgga tgcgcgcgcg ttcatcgtcc gcgtcctccg gcactggcgt tgagcccgtc
     4081 gttcaggccc tcgcccacca ggttgagcgc cagcaccgtg agcacgatgg ccaggccggg
     4141 gaaggccgcc atccaccagg cattgcgcag cacggtgcgg gccgcgccga tcatgtagcc
     4201 ccagctcatc atattgggat cgcccagccc catgaagctc agcgaggatt ccagcaggat
     4261 ggcggtggcg atcatcagcg aggccatcac cacgatgggc gacagcgcgt tgggcaggat
     4321 ctgcgtgagg atcacgcgcg gcgtcgattc gccggccagc agcgccgcgg agacgaagtc
     4381 gcgctggcgc agcgtgagga actcgccgcg caccaggcgg gccaccggct gccacgagac
     4441 caccgcgatg gccgccacga tggaatagat gctcggctcg aaaatggcca ccagcacgat
     4501 ggccagcgcg aagctgggca cggcctggaa catctcggta aagcgcatca gcgacgcgtc
     4561 gacccagccg ccgaaatagc cggcgatggc gcccaggctc acgccgatca gcagcgagac
     4621 cacggtggag atcacgccga tcagcagcga tacgcgggcg ccgtagatca ccccggagag
     4681 cacatcgcgg cccagcgtgt cggtgcccat gagcaggtgg ggctcgccca tcggcggcac
     4741 gaagggcgag gagaccatgt cccaagggtc gttggcggcg aagaaggggc cgaaggccgc
     4801 cagcagcacg atgaagatga ggaaggcgag ccccagggtc gcgccccgat tgcggcgaaa
     4861 gcgccgccag aaactatggt tcatgactgg tcttgggttg cgtgttgcat ggtgtcacga
     4921 caattcgatg cgcggatcca cgagcgtgta gacgagatcg gtaacgaggt tgaggagcac
     4981 ggccatggcc gaggtcacca ggaagcagcc cagcagcagg gtgtagtcgc gctgcagcag
     5041 cgcgtcgaac atcagccggc cgatgccggg ccaggcgaac acggtttcgg tgagcacggc
     5101 gccgcctatc atgccgccgg cctggatgcc ggccagcgtg accaccggca gcagggcgtt
     5161 gcgcaggatg tgcgcgcgct ggatgcgcgg cgggcgcagg cccttggcgc gcgccgtctt
     5221 gacgaaatcc atctgcgcca cttccagcat cgaggcgcgc gtcatgcggg cgtagacggc
     5281 catgtagaac agcgccagcg tgagcgaagg cagcaccagg tgctcgaaga tgtcccaggc
     5341 ttgcgccagg ccgctcaggc cggcgcccac cgtgaaatag ccgaagcccg gcagccagtc
     5401 gagcttgatc gagaacagca gcacggccat catcgccagc cagtacagcg gcgtggcata
     5461 gaacaccagg gccaccacgg tgatcgcgct gtcgacccag ctgccggcct tgcggctggc
     5521 ccaggcgccc agcgccacgc cgaagaccag cgacaggacg aaggcgctgc cggtcagcag
     5581 cagcgtggcg ggcaggcggt gcaggatcag gtcgagcacc ggctgctgct ggcggtagga
     5641 gtagccgagg tcgaggtgga cgatgtgatc gaggtagtgg ccaagctgca cgccgatcgg
     5701 ctggtcgagg ccgaactggc gcttgagctg ggcgacgaat tgcgggtcgg cggtgccggc
     5761 ttcgccggcc atcaccgaca ccggatcgcc cggggccgcg tgcaccagca ggaaattgac
     5821 cgtggcgatg gccaggatcg ccacgattcc ctgcgccagg cgcgtgagca tgaactggaa
     5881 ccgcttcatc tatgtactca ggaaagacgg ggtcttgcag gacggttcgg ccgccgcggg
     5941 cgcggcagcc gtcgtgccgg ggggttggcg tcaggcggtg agcttggcgt agccgaggcc
     6001 gtcgttcagg ccgatgccgg agctgatcag gttgtagacc ttggtgcggt acagggtcgg
     6061 gaagttgatt tcatgcagcc aggccaccgg cacatcgtcg accaggatct tctgcacttc
     6121 ttcgtagagg gccgtgcgct tggccgtgtc gacttcccgg cccgcgctgt cgaacagctc
     6181 gtcgaccttg ggattggcgt agccctcgac attgttgaag ggcgagccct tcgcgatgtt
     6241 gctggcggtg tagttgcgcg ccacccccag ggccgggtcg ccatactggt acacatagtt
     6301 gaaggccagg tcgtagtccc attggctcag gcgctggttc cagccggcca cgtcggtggc
     6361 caccatgtcc accttgacgc ccgcctgcgt caggttctgg cgcacgatct cggcctggcg
     6421 ctgccagctc tcgccgtagg gcaggggcag caggcgcagg gtctggccgg cgtagccgcc
     6481 atccttgagc agctgtttgg ccttggcaat gtcgtgggga tatttggcga ccttgtcggt
     6541 gtagtacttg atgtggctgt tgaacgggcc gatggcgggc ttggcatagc cttgccaggc
     6601 gatcttggcg atgctctcgt ggtcgatcgc ggtcatgatg gcctggcgca ccttgacgtt
     6661 gtccagcggc ttgttgcggt tgttcaggca cagccaggag tgcggcgcga agaactccca
     6721 gcccttggtg gtgacggccg cgccgggcag cttggccagg cgcgccacgt cgaagaactc
     6781 gaccgcgccg ccgggcacca cgtcgacctt gcccgattcg aaggcggccg cgcgcgaggc
     6841 cgcgtcgggg atc
//
LOCUS       DASWPO010000015        15949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1431337, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000015 DASWPO010000000
VERSION     DASWPO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431337"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGC69_00560"
     CDS             <1..541
                     /locus_tag="VGC69_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0293803.1"
                     /translation="TRRMRVPPHAGDFRLMDRKVVDAFVNLPERTRFMKGLYAWVGFR
                     SEPLPYVPKERLHGTSRFGFWRLFGLATAGITAFTTWPLRLVGLVGAAFAVLSFAYGV
                     FLVAQYLLYGNETSGWTTIVTALLFFSGINLLSLGVMGEYVARIFDEVKGRPLFIAGQ
                     RLGQARPRRDPPSDAPGRN"
     gene            561..2150
                     /locus_tag="VGC69_00565"
     CDS             561..2150
                     /locus_tag="VGC69_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0293804.1"
                     /translation="MIDTTAQPARRRLSGDALLLGLAAVWLLFLNWIRLLTLPDEGRY
                     TGVAWDMLRHGDLSTPLLNGLPYFHKPPLFYWLDAICMQLFGMNEWAVRVPSVLGAWL
                     ALFSTYAFVRRNRGVAAARVTVLVMATMPLFFGAAQFANLDMLVAGLISACVLAGADT
                     VLRRARGEPWRAMSILAGALAGLAILAKGLIGLVLPGAVLVFWLAMRRDGRGFLALLS
                     PPALLAFAVVAVPWCWLMQSRYPEFFHYFFIYQHFQRFAGSGFNNREPWLFYVPVLIG
                     TALPWTLWGLSLFRKPFWKADRDPDGIRRLMALWGLVVLVFFSIPASKLVGYILPALP
                     PLAFLLAEVVREAVERDGASSARYGRRASITSVAAVALCLITLTVAAISTFNRSAKPA
                     GVAMREAAGPDDTLVMLHTYAFDLPFYAYKRQPAWVVDNWDDPAILRTDSWRKELLDA
                     SEFNPAVKVLISDQEWKRRVCAGPDGARYWVWGEAQDAGRYPLLAGAQPFASTKRNVM
                     WRLEADAAFKQRVCGETPTAG"
     gene            complement(2110..2505)
                     /locus_tag="VGC69_00570"
     CDS             complement(2110..2505)
                     /locus_tag="VGC69_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0293805.1"
                     /translation="MHPLLKQLAWFVVVGCAAAATHWSVVVLLVEHAGLPPVLANVAG
                     WLIAFVVSFSGHYRLTFQHAGAPLLPAAGRFFLVSAMGFAVNEAAYAWLLHATPLPYD
                     LLLAAVLLGMAVLTFIASRLWAFRHKPAA"
     gene            2589..3377
                     /locus_tag="VGC69_00575"
     CDS             2589..3377
                     /locus_tag="VGC69_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEY0293806.1"
                     /translation="MNSAVDGGILYLAQAGRLGAVSCMALGPSFAGQAEALRGADVDA
                     GLHFDLTEGVGGQEPAIPLRHLIARAYLGRLAPTWVDGRLERQLDAFERLYGAAPAYL
                     DGHQHVHQLPGVLPRVIEILQRRYGQRMPWLRCTLPRSQPGVGAANAFKAQVIGMLGG
                     HALHRAVRRHGWRSNERFLGVYSLSGGSAHYAQLLRCWFEAARDGDLMMCHPARAVAP
                     GESDALMRQRAAEFDVLSNEDMPRWLQAQGLRVERLSRIAPLPA"
     gene            complement(3389..4279)
                     /locus_tag="VGC69_00580"
     CDS             complement(3389..4279)
                     /locus_tag="VGC69_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0293807.1"
                     /translation="MELRTLRALVEVVRQGGFSQAARVVFATQPTVSKAVKQLEDELG
                     MILLDRQSQPPKLTQAGEIVYRRALTMLAERDDLKAELDEIRGLKRGTLRLGLPPLGS
                     SSLFAPLFARFRSRYPSIEISLVEHGSRRLEEMLMTGELELAASLTPVPPDFEWQPVA
                     REPLMALLPPDHPHADAMHVSLWQLRQSPFILFEEGFALNHVLDDACRRAGVTPIVAA
                     RSGQIDFIIALVAAGLGVAFLPRLELRARSFTGVTCVPLDDPETDWDMGLVWRRGGYL
                     SHAAQAWLALARELRPGAAR"
     gene            4456..4857
                     /locus_tag="VGC69_00585"
     CDS             4456..4857
                     /locus_tag="VGC69_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEY0293808.1"
                     /translation="MTRTVSIRFRSLLRHSRLLQIGLIVLFSLVGQEIAGALGLSIPG
                     GVLGLVLVLGLLASGRLQVHAVRRGASWLLAEMLLFFVPAVMSVLDHHEFLGVLGLKL
                     LAAIVAGTAMVMVGTALTIDLGYRWMNRHSV"
     gene            4872..5567
                     /locus_tag="VGC69_00590"
     CDS             4872..5567
                     /locus_tag="VGC69_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEY0293809.1"
                     /translation="MRPEFELYFWPVATVLAYVLARWLYSRRPCLWTSTLVMAPALLL
                     ALAIWLHVGYSDYMLGSHWLMLMLGPVLVAFALPLYEQRAMILRYWPILAAGIVVGSV
                     IAGVSAWLLGSLLDLPTGLRLSLLPRSMATPFAMSVSSHVGGTPDLTAVFVIVTGVLG
                     ASIGQWLRAWLPLRSSLARGALFGMGAHGAGTAKARELAADEGAVAGLVMVMAGLFNV
                     LVTPYVVYGLMSL"
     gene            complement(5584..6219)
                     /locus_tag="VGC69_00595"
     CDS             complement(5584..6219)
                     /locus_tag="VGC69_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY0293810.1"
                     /translation="MNSLLALPLTSPALLGPLVLFALVSSITPGPNNAMLASSGLTFG
                     LQRTVPHLLGVSLGFSIMVLLVGVGLGAVFARQPLVYEALKYLGAAYLLFLAWKIANA
                     GPMREGAARARPITFLQAAMFQWVNPKAWVMAVGVIATYTPQQGFFGNLLIAALVCCL
                     VNLPCVGLWAVFGSALRRWLHRPDIVRGFNIAMALLLAASLYPVALELLGR"
     gene            6352..7794
                     /locus_tag="VGC69_00600"
     CDS             6352..7794
                     /locus_tag="VGC69_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0293811.1"
                     /translation="MTVHAAMPAAGWQPVREADATLVSQLTDDLARRIDDQGLRPGTR
                     LPSIRKMAEQAGVSRFTVVEAYDRLVARGLVQSRRGAGFFVRARDRQATAQAAQAGLQ
                     APTRIDPVWLLRSMFGPGSAPDMPGSMGLLPPDWLDAEMVAAAVRAVGRSARGHLLAY
                     GHPQGYEPLRQQIAALLQEQGLPAHPERNLLTTNGVTHGLDLVARHFVRPGDTVLVED
                     PAWFVIFGRLASMGARLIGIPRGPQGPDIAWLEKAAAEHKPKLFLINSAVHNPTGHTL
                     SAGAAYDILRVAERHDFLVVEDVTYGEMHPGSAIKLAELDRLNRVVLVGGFSKMLAAS
                     LRVGYLAADADTVQKLTDLKLMAGLTSCEVGERVVHRVLMEGQYRRHVERVRQRVDEA
                     RERCVKALLKLGFTVPHEPYAGMFVWADCGRDSEVMAREAAGRGMLLVPGSLFSPAQA
                     PSTMLRFAVSMVDDAKSWRELAALRRGRTA"
     gene            7855..8199
                     /locus_tag="VGC69_00605"
     CDS             7855..8199
                     /locus_tag="VGC69_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEY0293812.1"
                     /translation="MSAAIRPLSPTFAVAPQLAPEDMADVAAAGYKSVIINRPDYEGG
                     ADQPTAADVTRAAEALGLKVQYQPVVSGAMTMDDVTRFAELLRTLPGPVLAYCRTGTR
                     CTNLYANAQQLG"
     gene            8312..8767
                     /locus_tag="VGC69_00610"
     CDS             8312..8767
                     /locus_tag="VGC69_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0293813.1"
                     /translation="MFKKILIPTDGSPLSAQAVNAGITFARSVGAEVVGLYVTQPFAA
                     TIGFDGMAAAYAITDEDYEKASAEQAEKYLKQISDRASTAGVKATTRAVSNFNVADGI
                     VQAAADEHCDLIFIGSHGRSGLSRLLLGSVTAKVLTLSAVATLVFRVKE"
     gene            complement(8847..10052)
                     /gene="glp"
                     /locus_tag="VGC69_00615"
     CDS             complement(8847..10052)
                     /gene="glp"
                     /locus_tag="VGC69_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY0293814.1"
                     /translation="MLEFDEAQSQLTAAAPVPARTETVSLAQAAGRVLAAGLTATVDL
                     PSADNSAMDGYAIHHSDYQPGARLPIQQRCYAGEQPAPLIAGQATRLFTGSLMPQGAD
                     TVIMQEDTREADGTVEITAAPSTGQHVRKLGEDVRAGTPLLSAGALLQAAHIALLASQ
                     GLAQVEVYPRLRVGILTTGDELAVPGQPRQPGQVYNSNGPMLAALAQGLGADVVRVLH
                     ARDDMADLQAALRTLQSSCDLVVSVGGVSVGDHDLVKPAIESLGGELALWKVRMKPGK
                     PVAMARLDSKLIVCLPGNPVSAYAVFTVLVSPMIRRMQGRAQVYPPVLRAALRTEKTR
                     QDSREEFLRVQYHPLANGSADLTPYGHQGSGVISSLPWADGLARLPAGVPVNDGDIVA
                     YYDLKRWQT"
     gene            complement(10085..10612)
                     /gene="moaB"
                     /locus_tag="VGC69_00620"
     CDS             complement(10085..10612)
                     /gene="moaB"
                     /locus_tag="VGC69_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEY0293815.1"
                     /translation="MSSHTNDTISLACAILTVSDTRTAGDDTSGNLLAENVAQAGHVC
                     AKREIVRDDIYQIRRVMSDWIANPHIQVILATGGTGFSHKDSVPEAVKPLFDKEIDGF
                     GELFRQLSYAEIGSSTIQSRALAGYANNTVVFCMPGSNNACQMAWTHIIREQLDSRYK
                     PCNFATHLHRNRAPA"
     gene            complement(10609..11103)
                     /locus_tag="VGC69_00625"
     CDS             complement(10609..11103)
                     /locus_tag="VGC69_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY0293816.1"
                     /translation="MIIVQEADFDTAALTAALRERVGSQAGAIVTFTGYVRDYAPGEP
                     TETLTLEHYPGMCERELEDIAATAHLRWNLAGTVIAHRVGALRREAQIVFVAAASAHR
                     GEAFRGCEYMIDALKTRAPFWKRETLASGQAFWVEQRQADADRTQSWSATESPKETLS
                     KETP"
     gene            complement(11120..11368)
                     /gene="moaD"
                     /locus_tag="VGC69_00630"
     CDS             complement(11120..11368)
                     /gene="moaD"
                     /locus_tag="VGC69_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEY0293817.1"
                     /translation="MSAAVNLLYFARVAELVGKRTEAWPLAADTTGGHLLAELRARYP
                     RLAEARRLRLAVNQEHATPTARVQPGDEVAIFEPVTGG"
     gene            complement(11365..11733)
                     /locus_tag="VGC69_00635"
     CDS             complement(11365..11733)
                     /locus_tag="VGC69_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293818.1"
                     /translation="MCASLLALAGCVAVPVDQGGYYPAQPVYAAPEPAPALIYSPPPV
                     YIGPSDPVFVSPRRRYYGPAPRHYDPPRHYDPPRHYNAPRRSGPPEGRPRPSNPRPNN
                     NNRPQPRFVPDRGSGGSGQP"
     gene            complement(11847..12188)
                     /locus_tag="VGC69_00640"
     CDS             complement(11847..12188)
                     /locus_tag="VGC69_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEY0293819.1"
                     /translation="MKMSPFAFDTTDWSAVEKTEHQGETGMAYWRTRHVGDQANPIRV
                     RMVEYSPGYLADHWCSKGHILFCLEGELETTLADGRKFVLRPGMSYQVGDNAEAHQSR
                     THTGAKLFIVD"
     gene            complement(12185..12682)
                     /gene="moaC"
                     /locus_tag="VGC69_00645"
     CDS             complement(12185..12682)
                     /gene="moaC"
                     /locus_tag="VGC69_00645"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY0293820.1"
                     /translation="MTSPLPALSHLDESGQIRMVDVGAKADTERVAIASGRVRMNAQA
                     YGLLTAPGQGKGEVLNTARVAAVLAAKRCADLIPLCHSLPLAFVGVEFSLLDDEHAIE
                     VRATCRTHYKTGVEMEAMTACSVAALTIYDMCKAADKGIVIEDIHLEYKAGGKSGEWR
                     KRSTP"
     gene            12833..>15949
                     /locus_tag="VGC69_00650"
     CDS             12833..>15949
                     /locus_tag="VGC69_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY0293821.1"
                     /translation="MQLRMPVLKLSRRAGKILLGIMLVLLVLAGVAAWQVPRLVRNAL
                     TQNVSGLLGRQVAVGKISFNPFTLTLRAHELTIDQPQSPPLLQLGELDLSVAWRSIVL
                     FAPVVDMIHIDQPKLTLVRQDATHFNFSDIVDKLAKLSADKPVEPEPAKTALPRFSLN
                     NMSLTGGVISLDDHVTGRRQVVDELALGVPFISSFGYATHVDVQPKVHMRINGSPFDM
                     TGTARPFDKVPTSTLNVVFSGLELEKWADVWPMPLPVKVKRALLDSNLQVLFEQPPDA
                     APRIRVQGDLGLRQLDVTEASGAPLLDWDSLQVERIAVDPGAQTAYVGAVTLTGPRVD
                     VHRDAQQLNWQRVADGFAKLGGAPAAAPTPAAAAPASASAAAPAAAQPGWKVSVDDVK
                     LNAGEIHLRDDPTGLDYPLRGLAAEAENIAMPQAAGQPMHVWLNMDNDFDGSWLRAKA
                     PVVLQPLAVNAQVELSNLALAPFAAAVRHVAPMVVQDGRLGVKGKVNVAGSQVEARDV
                     QLDLQKLAVHDEAVKPGVDFSVGSLALAADRLAMDATPANFTLQADGIQKQGTLALKG
                     SFVPQPLSVKTSVDLADFDVASLAPYIASSLNATVRSVAVGARGDAEFTAAEGNAPMQ
                     VGWRGAVDVSNLDLEDRVNKAEFLNWKHLGLTNMNISMAGEKLGLGLGDILLEDFYGD
                     ILLNNDARLNVMDLMVDKGRAAGSITQDTQTRRSDAPQPAEAKPAAKAEAKPAPKPTG
                     NEPDISIASVTLKRGRMTFNDHFVRPNYRAELSAVEGTLTAVSSTKPVPAKVNITGRV
                     YGTAPLSVSGTVQPFAQYLALDIKASAKGVDLPKFTTYSSKYVGYAIQRGKLSVDLRY
                     QIKDRALQASNKVLLNQLTFGKQSNSPDAVKLPVLLAVALLKDSRGNIDIDLPISGSL
                     DDPQFSVGGIIIHVVMNLLVKAVTSPFQLLGSLFGGGDADLSYIDFPPGSAVLDDKAK
                     TSVGTLVKALTDRPALKMDVIGRADPQADEEGIRQAWVDGQLRMAKAKDTAGRGQKPD
                     PDSVTLTDA"
BASE COUNT         2386 a         5329 c         5608 g         2626 t
ORIGIN      
        1 cacgcgccgc atgcgggtgc cgccgcatgc gggcgacttc cgcctgatgg accgcaaggt
       61 ggtcgacgcg ttcgtcaacc tgcccgagcg cacgcgcttc atgaaaggcc tgtacgcctg
      121 ggtgggcttt cgttccgagc ccttgccgta tgtgcccaag gagcgcctgc acggcaccag
      181 ccgcttcggc ttctggcgct tgttcggcct ggccaccgcc ggcatcacgg cattcacgac
      241 ctggccgctg cggctggtgg gcctggtggg cgcggccttt gccgtgctgt cgtttgccta
      301 cggcgtcttc ctggtggcgc agtacctgct ctacggcaac gagacctcgg gctggacgac
      361 catcgtgacg gcgctgctgt tcttctcggg catcaacctg ctttcgctgg gggtgatggg
      421 cgagtacgtg gcgcgcatct tcgacgaggt caagggccgg ccgctgttca tcgccggcca
      481 gcggctcggg caggcgcggc cgcgccgcga tccgccgtcc gacgcgcccg gccggaactg
      541 agtaaaccca tatgagttcc ttgatcgaca ctactgcgca gccggcgcgc cggcgcctgt
      601 ccggcgacgc cctgctgctg ggcctggccg ccgtctggct gctctttctc aactggatac
      661 ggctgctgac gctgcccgac gaggggcgct acaccggcgt ggcctgggac atgctgcgcc
      721 atggcgacct ctccacgccg ctgctcaacg gcctgccgta tttccacaag ccgcccctgt
      781 tctattggct ggatgcgatc tgcatgcagc tcttcggcat gaacgagtgg gccgtgcgcg
      841 tgccttccgt gctgggcgcc tggctggcgc tgttctcgac ctacgccttc gtgcgccgca
      901 accgcggcgt ggcggcggcg cgcgtgaccg tgctggtcat ggccaccatg ccgctgttct
      961 tcggcgcggc gcagttcgcc aacctcgaca tgctggtggc cggcctgatc tcggcctgcg
     1021 tgctggccgg ggccgacacg gtgttgcggc gtgcccgcgg cgaaccctgg cgcgccatgt
     1081 cgatactggc gggcgcgctg gccgggctgg ccatcctggc caagggcctg atcggcctgg
     1141 tgctgcctgg cgccgtgctg gtgttctggc tggcgatgcg gcgcgacggg cgcggttttc
     1201 tcgcgctgtt gtcgccgccg gcgttgctgg cctttgccgt ggtggccgtg ccctggtgct
     1261 ggctgatgca gtcgcgctat ccggagttct tccactactt cttcatctac cagcacttcc
     1321 agcgctttgc tggctcgggc ttcaacaacc gggagccctg gctcttctac gtgccggtgc
     1381 tgatcggcac ggccctgccc tggacgctgt ggggcctgag cctgtttcgc aagcccttct
     1441 ggaaggccga ccgcgacccc gacggcatac gccgcctcat ggccctgtgg gggctggtgg
     1501 tgctggtgtt cttctcgatc ccggcatcca agctggtggg ctacatcctg ccggccctgc
     1561 cgcccctggc cttcctcctg gccgaggtgg tgcgcgaggc ggtcgagcgc gatggcgcgt
     1621 cttccgcgcg ctacggccgg cgcgcctcga tcacttccgt ggcggccgtg gcgctttgcc
     1681 tgatcacgct gaccgtggcc gcgatcagca cgttcaaccg cagcgccaag ccggcgggcg
     1741 tggccatgcg cgaggcggcc gggccggacg acacgctggt catgctgcac acctatgcct
     1801 tcgacttgcc gttctatgcc tacaagcgcc agccggcctg ggtggtcgac aattgggacg
     1861 atccggcgat cctgcgcacc gacagctggc gcaaggaact gctcgatgcc tccgaattca
     1921 atccggccgt caaggtgctg atctcggatc aggaatggaa gcggcgcgtc tgcgcgggcc
     1981 cggacggcgc gcgctattgg gtctggggcg aggcgcagga cgcgggccgc tatcccttgc
     2041 tggcgggcgc ccagccgttt gcatcgacca agcgcaacgt gatgtggcgc ctggaggccg
     2101 acgcggcatt caagcagcgg gtttgtggcg aaacgcccac agccggctag cgatgaaagt
     2161 caggacggcc atgccgagga gcacggccgc cagcagcagg tcgtagggca gcggcgtggc
     2221 atgcagcagc caggcatagg cggcctcgtt gacggcgaag cccatggccg agacgaggaa
     2281 gaagcgtccc gcggcgggca gcaagggcgc gccggcgtgc tggaaggtaa ggcggtagtg
     2341 gccggaaaac gagaccacga aagcgatcag ccatccggcg acattggcca gcacgggcgg
     2401 caggccggcg tgttcgacca gcagtacgac cacgctccaa tgggtcgcgg cggcggcgca
     2461 gccgacgacg acaaaccagg cgagttgttt taacagcggg tgcatcaagc gtggaaaacc
     2521 aggaaaacgg gcaacaagcc aacccacagg ggcggcgccg catcgtcatt tgcgccgacg
     2581 attttggcat gaattcggct gtggatggcg gcatcctgta tctggcgcag gccgggcgcc
     2641 tgggcgcggt gagctgcatg gcgctgggtc cgtcgtttgc cgggcaggcc gaggccctgc
     2701 gtggcgcgga tgtcgacgcg ggcctgcatt tcgatctcac cgaaggcgtg ggcgggcaag
     2761 agcccgccat tccgctcagg catctcatcg cgcgcgccta tcttgggcgc ctggcgccga
     2821 cctgggtgga cggccggctg gaacgccagc tcgatgcctt cgagcggctc tacggcgcgg
     2881 cgccggccta tctggatggg catcagcacg tgcatcaatt gccaggggtg ctgccgcggg
     2941 tgatcgagat cctgcagcgc cgctacggcc agcgcatgcc ctggctgcgc tgcaccttgc
     3001 cgcgcagcca gcccggcgtg ggggcggcca atgccttcaa ggcgcaagtc atcggcatgc
     3061 tgggtggcca cgccctgcac cgggccgtgc gccggcacgg ctggcgcagc aatgagcgct
     3121 tcctgggggt ctacagcctg agcggcgggt cggcgcatta tgcgcagctg ctgcgctgct
     3181 ggttcgaggc ggcccgggac ggcgacctga tgatgtgcca tcccgcccgg gcggtcgcgc
     3241 cgggcgagag cgacgcgctc atgcggcagc gcgccgccga gttcgacgtg ctgtcgaacg
     3301 aagacatgcc ccgctggctg caggcccagg gcctgcgcgt ggagcggctc agccgcattg
     3361 cgccgctgcc ggcctgaagc gcggcgcgtc agcgcgcggc gcccggacgc agttcgcgcg
     3421 ccagggccag ccaggcttgc gcggcgtgcg acaggtagcc gccgcggcgc cacaccaggc
     3481 ccatgtccca gtcggtttcc ggatcgtcca gcggcacgca ggtgacgccg gtgaacgagc
     3541 gcgcccgcag ttccaggcgc ggcaggaagg ccacgcccag gccggccgcc accagggcga
     3601 tgatgaagtc gatctggccg ctgcgggccg ccacgatggg cgtgacgccg gcgcgccggc
     3661 aggcgtcgtc cagtacatga ttcagcgcga agccttcttc gaagaggatg aagggcgact
     3721 gccgcagctg ccacaggctc acgtgcatgg cgtcggcatg cgggtggtcg ggcggcagca
     3781 gggccatgag cggttcgcgc gcgacgggtt gccattcgaa gtccggcggc acgggggtga
     3841 gcgaggcggc cagctccagc tccccggtca tcagcatttc ttcgaggcgc cggctgccgt
     3901 gctcgaccag gctgatctcg atggaagggt agcggctgcg aaagcgcgca aagagcgggg
     3961 cgaacagcga actgctgccc aggggcggca ggcccaggcg caaggtgccg cgcttgaggc
     4021 cgcggatttc gtcgagctcg gccttcagat cgtcgcgttc cgccaacatg gtgagcgcgc
     4081 gccggtacac gatttccccg gcctgggtga gcttgggcgg ctgggattgg cggtccagca
     4141 atatcatgcc cagctcgtct tcgagctgct tgacggcctt gctgacggtg ggctgggtgg
     4201 cgaaaaccac ccgggccgcc tgggagaagc cgccctggcg gacgacttcc accagggcgc
     4261 gcagggtacg tagttccatg agctagggct ccatgttgcg gtgcggcatc ggaatccagc
     4321 caaagctgtc ggcttgttcc ccatgcattc caaagaagaa tactaaaaat tcaaacaatt
     4381 cattttatct ataagccggg caggcgtaca acataaggcg agccaagcat ggctcaccgc
     4441 ccaggagtat cccccgtgac ccgcaccgtt tccatccgat ttcgtagctt gctgcgccac
     4501 agcaggctgc tgcagatcgg cctgatcgtc ctgttctcgc tggtcggcca ggaaatcgcc
     4561 ggtgcgctgg gcctgtcgat ccccggcggg gtgctgggcc tggtcttggt gctgggcctg
     4621 ctggccagcg gcaggctgca ggtgcatgcc gtgcgccgcg gcgccagctg gctgctggcc
     4681 gagatgctgc tgttcttcgt gccggcggta atgtccgtgc tcgatcacca tgaattcctg
     4741 ggcgtgctgg ggctcaagct gctggccgcc atcgtggcgg gcacggccat ggtcatggtg
     4801 ggcaccgcgc tcaccatcga cctgggctat cgctggatga accgccactc ggtttgagtc
     4861 caggcctgat catgcgcccc gagtttgaac tctatttctg gccggtggcc acggtgctgg
     4921 cctatgtgct ggcgcgttgg ctgtacagcc gccgcccttg cctctggacg tccaccctgg
     4981 tcatggcccc ggccctgctg ctggccctgg cgatctggct gcacgtgggc tatagcgact
     5041 acatgctggg ctcgcactgg ttgatgctga tgctgggccc ggtgctggtg gctttcgcgc
     5101 tgcccctgta cgagcagcgc gccatgatcc tgcgctattg gcccatcctg gcggcgggca
     5161 tcgtggtggg cagcgtgatc gccggcgtga gcgcctggct gctgggcagc ctgctcgacc
     5221 tgccgaccgg cctcaggctg agcctgctgc cgcgctccat ggccacgccg ttcgccatgt
     5281 cggtgtcctc gcacgtgggc ggcacccccg acctgaccgc cgtgttcgtg atcgtcaccg
     5341 gcgtgctggg cgccagcatc ggccaatggc tgcgcgcctg gctgccgctg cggtcatccc
     5401 tggcgcgcgg cgcgctgttc ggcatgggcg cgcatggcgc cggcacggcc aaggcccgcg
     5461 aactggcggc cgacgagggg gccgtggccg gcctggtcat ggtgatggcc gggctgttca
     5521 atgtgctggt cacgccctat gtggtgtacg gcctgatgtc gctctgaggg gcggggcttc
     5581 gcgtcagcgc cccagcagtt ccagcgccac cgggtagagc gaggccgcca gcagcagcgc
     5641 catcgcgata ttgaagccgc gcacgatgtc cgggcggtgc agccagcggc gcagggcgct
     5701 gccgaagacg gcccacaggc ctacgcaagg cagattgacc aggcagcaca ccagggcggc
     5761 gatcagcagg ttgccgaaga acccctgttg cggcgtatag gtggcgatca cgcccaccgc
     5821 catcacccat gccttgggat tgacccactg gaacatcgcg gcctgcagga aggtgatggg
     5881 ccgggcgcgc gccgcgcctt cccgcatggg gccggcgttg gcgatcttcc aggccaggaa
     5941 cagcaggtat gcggcgccga ggtacttgag ggcctcgtac accaggggtt ggcgcgcgaa
     6001 caccgcgccc aggccgacgc ccaccaacag caccatgatg gaaaagccca ggctcactcc
     6061 cagcaggtgc ggcacggtgc gctgcaggcc gaaggtcagg ccggacgagg ccagcatggc
     6121 gttgttgggg ccgggcgtga tcgaactgac cagggcgaac aggaccagcg ggcccagcag
     6181 ggcgggggag gtcagcggca gggcaagcag ggaattcatg atgcggcgac ggcaaggggg
     6241 caggcggatg cttgcccgtt gtgatgattt ctgccactgt atgcatagtt aacgatacag
     6301 taccggtaca attatccagt tcactttcaa cactggcccg gtagaaacgc catgacagtt
     6361 cacgccgcca tgcccgcggc cggttggcag ccggtacgcg aggccgatgc caccctggtc
     6421 agccagttga ccgacgacct ggcccgccgc atcgacgacc agggcctgcg ccccggcacg
     6481 cgcctgccct ccatccgcaa gatggccgaa caggccggcg tgagccgctt cacggtcgtc
     6541 gaagcctacg accgcctggt ggcgcgtggc ctggtgcagt cgcgccgcgg cgccgggttc
     6601 ttcgtcaggg cccgggaccg gcaggccacc gcgcaagccg cgcaggccgg tttgcaggcg
     6661 cccacgcgca tcgatccggt gtggctgctg cgcagcatgt tcggcccggg ttcggcgccc
     6721 gacatgccgg gcagcatggg cctcttgccg cccgactggc tggacgccga gatggtggct
     6781 gccgccgtgc gcgccgtggg ccgttcggcg cgcgggcact tgctggccta cggccatccg
     6841 cagggctacg agcccctgcg ccagcagatc gccgccctgc tgcaggagca gggcctgccc
     6901 gcgcaccccg agcgcaacct gctcaccacc aacggcgtca cgcatgggct ggacctggtg
     6961 gcgcgccatt tcgtgcggcc gggcgatacc gtgctggtcg aagacccggc ctggttcgtg
     7021 atcttcggcc gcctggcctc gatgggcgcg cgcctgatcg gcataccgcg cggcccgcag
     7081 ggccccgaca tcgcctggct ggagaaggcg gccgccgagc acaagcccaa gctcttcctc
     7141 atcaacagcg ccgtgcacaa ccccaccggg cacaccctgt cggcgggggc ggcctacgac
     7201 atcctgcgcg tggccgagcg gcacgacttc ctggtggtcg aggacgtcac ctacggcgaa
     7261 atgcacccgg gcagcgccat caagctggcc gagctcgacc ggctcaaccg cgtcgtcctg
     7321 gtgggcggct tttccaagat gctggccgcc agcctgcggg tgggctacct ggccgccgat
     7381 gccgacaccg tgcagaaatt gaccgacctc aagctgatgg ccgggctcac gtcctgcgaa
     7441 gtgggcgagc gcgtcgtgca ccgcgtgctg atggaggggc agtaccgccg ccatgtggag
     7501 cgcgtgcgcc agcgcgtgga cgaggcgcgc gagcgctgcg tgaaggcttt gctcaagctg
     7561 ggctttaccg tgccgcacga accctacgcg ggcatgttcg tctgggccga ttgcggccgc
     7621 gattccgagg tcatggcgcg cgaggcggcc gggcgcggca tgctgctggt gccgggctcg
     7681 ctgttctcgc cggcgcaggc gccgtcgacc atgctgcgct ttgccgtgtc catggtcgac
     7741 gatgccaaga gctggcgcga gctggcggcc ctgcggcgcg ggcggacggc ctagccggca
     7801 tatactttcc ccattcgaac gtttcccatt cgagccgcct ttcaggaagc catcatgtcc
     7861 gctgccatcc gtccgctttc cccgaccttt gccgttgccc cccagctcgc gcccgaagac
     7921 atggccgatg tcgcggcggc cgggtacaag agcgtcatca tcaaccgccc cgactacgaa
     7981 ggcggcgccg accagcccac cgcggccgac gtcacgcgcg cggccgaggc gctgggcctg
     8041 aaggttcaat accagcccgt ggtcagcggc gccatgacca tggacgacgt gacgcgtttt
     8101 gccgaactgc tgcgcacgct gcccggtccc gtcctggcct attgccgcac cggcacgcgc
     8161 tgcaccaacc tctacgccaa cgcccagcaa ctgggctgac gcggcagcgg cattcatgca
     8221 ttcctgaggc ctgttttgcg gcgtgtatca ggtgtgacgc agcgcgacct tttccggtag
     8281 catggaaaca ctgctcataa ggagttggac catgttcaag aagatactga ttcccaccga
     8341 cggttcgccg ctgtcggccc aggccgtcaa tgccggcatc actttcgcgc gctccgtggg
     8401 cgccgaggtg gtggggctgt acgtgacgca gcccttcgcg gccaccatcg gcttcgatgg
     8461 catggcggcg gcctatgcca tcaccgacga agactacgaa aaggcctcgg ccgagcaggc
     8521 cgaaaagtac ctcaagcaaa tcagcgaccg cgccagcacc gcgggcgtga aagccaccac
     8581 gcgcgccgtg tccaacttca acgtggccga cggcatcgtg caggccgcgg cggacgagca
     8641 ctgcgacctg atcttcatcg gcagccatgg ccgcagcggc ctgtcgcgcc tgctgctggg
     8701 cagcgtcacg gccaaggtgc tgacgctgtc cgccgtcgcc acgctggtgt tccgcgtcaa
     8761 ggagtgatcg gccggcgtga tcgccgccga tagcaaagaa gccgtccgga tgccggacgg
     8821 cttcttttat ttgcgcctgc gggcaatcag gtctgccagc gcttcaaatc gtagtaggcc
     8881 acgatgtcgc cgtcgttgac cggcacgccg gccggcaggc gcgccaggcc gtcggcccag
     8941 ggcagcgagc tgatcacgcc cgagccctgg tgcccgtagg gcgtcaggtc ggcgctgcca
     9001 ttggccagcg ggtggtattg cacgcgcagg aattcttcgc ggctgtcctg gcgggttttt
     9061 tccgtgcgca gcgcggcgcg cagcaccggt ggatagacct gcgcgcggcc ctgcatgcgg
     9121 cggatcatgg gcgagaccag cacggtgaac accgcgtagg ccgacaccgg attgcccggc
     9181 aggcagacga tgagcttgct gtccagccgg gccatggcca cgggcttgcc cggcttcatg
     9241 cgcaccttcc agagcgccag ttcgccgccc agcgactcga tggccggctt gaccaggtcg
     9301 tggtcgccca ccgacacgcc gcccacgctg acgacgagat cgcaggacga ttgcagcgtg
     9361 cgcagggccg cctgcaggtc ggccatgtcg tcgcgcgcgt gcagcacgcg cacgacgtcc
     9421 gcgcccaggc cttgcgccag ggcggccagc atggggccgt tggagttgta gacctggcca
     9481 ggctggcgcg gctggccagg caccgccaat tcgtcgccgg tggtcaggat gcccacgcgc
     9541 aggcgcgggt agacctcgac ctgggccagg ccctgcgaag ccagcagcgc gatatgcgcg
     9601 gcctgcagca aggcgccggc gctcagcagc ggcgtgccgg cgcgcacgtc ctcgcccagc
     9661 ttgcgcacgt gctggccggt gctcggcgcc gcggtgatct cgaccgtgcc gtcggcctcg
     9721 cgcgtgtctt cctgcatgat cacggtgtcg gcgccctgcg gcatcaggct gccggtgaac
     9781 aggcgcgtgg cctggccggc gatcagcggc gcgggttgtt cgcccgcgta gcagcgttgc
     9841 tggatgggca gcctggcgcc gggctggtag tcggaatgat ggatggcata gccgtccatg
     9901 gcgctgttgt cggcggaggg caggtcgacc gtggccgtga ggccggccgc cagcacgcgg
     9961 ccggcggcct gggccaggga aacggtttcg gtgcgggcgg gaacgggggc ggctgcggtg
    10021 agctgcgact gggcctcgtc gaattcgagc atggagagtc tggttggaga gtcaggcccg
    10081 ggcatcaggc cggggcgcgg ttgcggtgca ggtgggtggc gaaattgcag ggcttgtagc
    10141 ggctgtcgag ctgctcgcgg atgatgtgcg tccaggccat ctggcacgcg ttgttggagc
    10201 ccggcatgca gaagaccacg gtgttgttgg catagcccgc cagcgcgcgc gactggatgg
    10261 tggagctgcc gatctcggca tacgacagct ggcggaacag ttcgccgaag ccgtcgattt
    10321 ccttgtcgaa caggggcttg acggcttcgg gcacggaatc cttgtgcgag aagcccgtgc
    10381 cgcccgtggc gaggatcacc tggatgtgcg ggttggcgat ccagtcgctc atcacgcggc
    10441 ggatctggta gatgtcgtcg cgcacgatct cgcgcttggc gcagacgtgg ccggcctgcg
    10501 ccacgttttc ggccagcagg ttgcccgagg tgtcgtcgcc cgcggtgcgc gtgtcgctca
    10561 cggtgagaat ggcgcaggca agcgagatgg tgtcgttggt atggctgctc atggggtttc
    10621 cttggacagt gtttccttgg gcgactccgt ggcgctccag gattgggtgc ggtccgcgtc
    10681 ggcctgtcgc tgctcgaccc agaacgcctg ccccgaagcc agggtctcgc gcttccagaa
    10741 gggcgcccgg gtcttgagcg cgtcgatcat gtattcgcag ccgcgaaagg cctcgccgcg
    10801 atgcgcgctg gccgcggcca cgaaaacgat ctgcgcttcg cgccgcaggg cgccgacgcg
    10861 atgggcgatc acggtgccgg ccaggttcca gcgcaggtgc gccgtggcgg cgatgtcttc
    10921 gagctcgcgc tcgcacatgc ccgggtaatg ctccagggtc agcgtctcgg tcggttcgcc
    10981 gggcgcgtag tcgcgcacat agccggtaaa ggtgacgatg gcgccggcct ggctacccac
    11041 gcgctcgcgc agggcggccg tcagcgccgc ggtgtcgaaa tcggcttcct ggacgatgat
    11101 catggctcgg ctcccgcgtt caaccgccgg tcacgggttc gaagatcgcc acttcgtcgc
    11161 cgggctgcac gcgcgccgtg ggggtggcat gttcctggtt gacggccagg cgcaggcgcc
    11221 gggcctcggc caggcgcggg tagcgcgcgc gcaactcggc gagcagatgt ccgccggtgg
    11281 tgtcggcggc caagggccac gcctcggtgc gcttgcccac cagctccgcc acgcgggcga
    11341 agtacagcag gttgacggcg gcgctcatgg ctggccgctg ccgccgctcc cgcggtcggg
    11401 aacgaaacgg ggttgcggac ggttgttgtt gttgggccgc gggttgctcg ggcgcggacg
    11461 gccctcgggc gggcccgagc gtcgcggtgc gttgtagtgg cgtggcgggt cgtagtggcg
    11521 tggcgggtcg tagtggcgcg gcgccggtcc gtagtagcgg cgccgcggcg acacgaaaac
    11581 cgggtcggac gggcctatat acaccggtgg cggcgagtag atgagggcgg gcgcgggctc
    11641 aggcgcggca tagaccggct gggcagggta atagcccccc tgatcgaccg gaaccgccac
    11701 gcagccggcc agggccagca gggatgcgca caccgccgca ttgcggatga tcgccatgat
    11761 gtcaattctc cttgcgcagc ttgccgctct tcggacgagt gtaacagggc tcgatgcggg
    11821 cgccgtcgcg cgccgcggga cgcgcctcag tcgacgatga agagcttcgc gccggtatgc
    11881 gtgcgggact ggtgcgcttc ggcgttgtcg cctacctggt agctcatgcc tggccgcagc
    11941 acgaacttgc ggccatctgc aagcgtcgtt tccagttcgc cttccaggca gaaaaggatg
    12001 tggcccttgc tgcaccagtg atcggccagg tagccggggc tgtactcgac catgcgcacg
    12061 cggatgggat tggcttggtc gccgacatgg cgcgtgcgcc aataggccat gccggtttcg
    12121 ccttggtgtt cggttttctc gacggcggac caatcggtgg tgtcgaaggc gaagggggac
    12181 attttcatgg ggtgctcctt ttgcgccact cgccgctctt gccgccggcc ttgtattcca
    12241 ggtggatgtc ctcgatcacg atacctttat cggcggcctt gcacatgtca taaatggtga
    12301 gcgccgcaac gctgcaggcc gtcatcgctt ccatctcgac gccggtcttg tagtgggtgc
    12361 ggcaggtggc gcgcacctcg atggcatgtt cgtcgtcgag caggctgaac tccacgccca
    12421 cgaaagccag cggcaggctg tggcacaggg ggatgaggtc ggcgcagcgc ttggcagcca
    12481 gcacggcggc cacccgggcg gtgttgagca cctcgccctt gccctgtccg ggggcggtga
    12541 gcaggccata ggcctgggcg ttcatgcgca cccggccgct ggcgatggcg acccgttcgg
    12601 tgtcggcctt ggcgccgacg tcgaccatgc ggatctggcc ggattcgtcc aggtggctca
    12661 gcgcggggag gggggacgtc atgagtaaag aatagtgact atgtcgcaaa agcctatgat
    12721 agacgagccc ggggcgagac ggtgataatg agggccaaag ccgcccgagg cattgcggcg
    12781 gcgccaatgt gaggcgcaca cggggcgcaa aacctagtcg ggagggtgtg caatgcagct
    12841 gaggatgccg gttctgaaac tcagccgacg ggcgggaaag atactgctcg gcatcatgct
    12901 ggttctgctg gtgctggccg gcgtggccgc ctggcaggtg cccaggctgg tgcgcaacgc
    12961 gctcacccag aatgtctccg ggctgctggg ccgccaggtg gcggtgggca agatcagttt
    13021 caaccccttc acgctgacgc tgcgcgcgca cgaactcacc atcgaccagc cccagtcgcc
    13081 gcccttgctg cagctcggcg agctcgacct cagcgtggcc tggcggtcca tcgtgctgtt
    13141 cgcgccggtg gtcgacatga tccacatcga ccagcccaag ctcacgctgg tgcgccagga
    13201 cgccacgcat ttcaattttt ccgacatcgt cgacaagctt gccaagctgt cggccgacaa
    13261 gccggtcgag cccgagccgg ccaagacggc gctgccgcgc ttttccctga acaatatgtc
    13321 gctgaccggc ggcgtcatca gcctggacga ccacgtcacc ggccgccggc aggtggtcga
    13381 cgaactcgcg ctgggcgtgc cgttcatttc ctcgttcggc tatgccacgc acgtcgacgt
    13441 gcagcccaag gtgcacatgc gcatcaacgg cagccccttc gacatgaccg gcacggcgcg
    13501 gcccttcgac aaggtgccca cttccaccct gaacgtggtg ttttccgggc tggagctcga
    13561 gaagtgggcc gacgtctggc ccatgccgct gcccgtcaag gtcaagcgcg ccctgctcga
    13621 ctccaatctg caggtgctgt tcgagcagcc gcccgacgcg gcgccgcgca tccgcgtgca
    13681 aggcgacctg ggcctgcgcc agctcgacgt gaccgaggcc tcgggggcgc cgctgctgga
    13741 ctgggactcc ctgcaggtcg agcgcatcgc ggtcgatccg ggcgcgcaga cggcctatgt
    13801 cggggcggtg accctgacgg ggccgcgcgt ggacgtgcac cgcgacgccc agcagctgaa
    13861 ctggcagcgc gtggccgacg gcttcgccaa gctgggcggg gcgcccgccg ccgcgcccac
    13921 ccctgcggcc gccgcgccgg cttccgcttc tgcggcggcg cccgctgccg cccagcccgg
    13981 ctggaaagtc tccgtcgacg acgtcaagct caacgccggc gagatccacc tgcgcgacga
    14041 cccgaccggc ctggactacc cgctgcgcgg cctggccgcc gaggccgaga acatcgccat
    14101 gccgcaggcc gcaggccagc ccatgcatgt gtggctgaac atggacaacg atttcgacgg
    14161 cagctggctg cgagccaagg cgcccgtggt cttgcagccc ctggcggtga acgcgcaggt
    14221 cgaactgagc aacctggccc tggcgccctt cgcggcggcg gtgcgccacg tcgcgcccat
    14281 ggtcgtgcag gacggccggc tgggcgtgaa gggcaaggtc aacgtggcgg gttcgcaggt
    14341 cgaggcgcgc gacgtgcagc tcgacctgca aaagctcgcc gtgcatgacg aggccgtcaa
    14401 gcccggcgtg gatttttccg tcggctcgct ggcgctggcc gccgaccggc tggccatgga
    14461 tgccacgccg gccaacttca cgctgcaggc cgacggcatc cagaagcagg gcacgctggc
    14521 gctcaagggc tcgttcgtgc cgcagccgct gtcggtgaag acctcggtgg acctggccga
    14581 tttcgacgtg gcctcgctgg cgccctatat cgcctccagc ctgaacgcca cggtgcgcag
    14641 cgtggccgtg ggcgcgcgcg gcgatgccga atttaccgcg gcagagggca acgcgcccat
    14701 gcaggtgggc tggcgcggcg ccgtcgacgt cagcaacctc gatctcgaag accgcgtcaa
    14761 caaggccgag ttcctcaact ggaagcacct gggcctgacc aacatgaata tctcgatggc
    14821 gggcgaaaag ctcggcctgg ggttgggcga catcctgctg gaagacttct acggcgacat
    14881 cctgctcaac aacgacgcgc gcctgaacgt catggacctc atggtggaca agggccgggc
    14941 cgccggttcc atcacgcagg acacgcagac gcgccgcagc gacgcgcccc agcccgccga
    15001 agccaagccc gccgccaagg ccgaggccaa gccggcgccc aagcctaccg gcaacgagcc
    15061 cgacatctcg atcgccagcg tcacgctcaa gcgcggccgc atgaccttca acgaccattt
    15121 cgtgcggccc aactaccgcg ccgaactgtc ggccgtcgaa ggcacgctca ccgcggtgtc
    15181 gtcgaccaag ccggtgccgg ccaaggtcaa catcacgggc cgggtgtacg gcacggcgcc
    15241 cctgtcggtg tcgggcacgg tgcagccttt cgcgcaatac ctggcgctgg acatcaaggc
    15301 gtcggccaag ggcgtggacc tgcccaagtt caccacctat tcgtccaagt acgtgggcta
    15361 tgccatccag cgcggcaagc tgtcggtcga cctgcgatac cagatcaagg accgcgccct
    15421 gcaggccagc aacaaggtgc tgctcaacca gctcaccttc ggcaagcagt ccaacagccc
    15481 cgatgccgtc aagctgccgg tgctgctggc cgtggccttg ctgaaggaca gcaggggcaa
    15541 catcgatatc gacctgccca tttccggctc gctggacgac ccgcagttct cggtgggcgg
    15601 catcatcatc cacgtggtga tgaacctgct ggtcaaggcg gtgacctcgc ccttccaact
    15661 gctgggctcg ctcttcgggg gcggcgacgc ggacctgtcg tacatcgact tcccgcccgg
    15721 cagcgccgtg ctcgacgaca aggccaagac cagcgtcggc accctggtca aggcgctgac
    15781 cgaccgcccc gcgctgaaga tggacgtcat cggccgcgcc gatccgcagg ccgacgagga
    15841 aggcatccgc caggcctggg tggacggcca gctgcgcatg gccaaggcca aggacaccgc
    15901 cggtcgcggg cagaagcccg accccgatag cgttacgctg accgacgcc
//
LOCUS       DASWPO010000016         3673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_539026, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000016 DASWPO010000000
VERSION     DASWPO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539026"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="tmk"
                     /locus_tag="VGC69_00655"
     CDS             <1..600
                     /gene="tmk"
                     /locus_tag="VGC69_00655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY0293822.1"
                     /translation="EGVDGAGKSTHAGWLVEAIRAAGHTVLATREPGGTPIGEKLREL
                     VLHEPMRLETETLIMFAARCEHLRTVIEPALARGEWVVCDRFTDASYAYQGGGRELGA
                     APVAALEAWIGAPQPDRTWLFDVPLAVARARLAASRDPDRFEREAGAFFERVRAAYQA
                     RAQAHPERVRVIDGSQPMADVRARLEVDLRELLRQGPGA"
     gene            597..1634
                     /gene="holB"
                     /locus_tag="VGC69_00660"
     CDS             597..1634
                     /gene="holB"
                     /locus_tag="VGC69_00660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY0293823.1"
                     /translation="MSGFLPWHLETARAWLAQRDRFAHAWLLHGEAGIGKQDFAVAAA
                     ASLLCETPQQGLACGHCIACGWFASGNHPDLRRIRPEAVALTEGAQASESDEAETAAA
                     GSKRAPSKDIRIDQIRSLEPWFNTATHRGGWRVALLYPAQALNTIAANALLKVLEEPP
                     AHTVFLLVSDAPDRLLPTLVSRCRRLPLAAPPAETALQWLAGQGVKQPADWLAAAGGT
                     PLSALRLSQKQDEPCPGWLGQLLTPLAQGHRPDVGSLTETLEKVAAPEWLDALQRVFT
                     DLMLAANGLSVRYFPALAELTAHTAARVQIARLADTAHWLVSQRAVAGHPLNPKLFIH
                     ATLQRVALALA"
     gene            1642..2412
                     /locus_tag="VGC69_00665"
     CDS             1642..2412
                     /locus_tag="VGC69_00665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0293824.1"
                     /translation="MYVDSHCHLNFPDLAQDLPGVLERMRQNQVTHALLASVTMPSWP
                     GLMEIVESTPNLWGSVGVHPDEENTPEPDVDELVRLARHPKVVAIGETGLDYYRLSEP
                     LDWQRERFRRHIRAARQAKLPLIIHTRNSAADTVRILAEEGAEEIGGVMHCFTETWEI
                     AQQALDLNFYISMSGIVTFKNAQVVQDVATRTPLDRLLIETDSPYLAPVPYRGKLNDP
                     AKVVYVAEKVADLKGLGSADIAKQTSENFFKLFNKIEK"
     gene            2482..3327
                     /locus_tag="VGC69_00670"
     CDS             2482..3327
                     /locus_tag="VGC69_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0293825.1"
                     /translation="MMTQPAFRRRLRAALAALCLAAPIWAGSVAHAQATPPDWWVDIE
                     NDRADKIRSELAQGVDPNLKASNGQPALLRAVASDSWKAFDALLADPRTDVNAENPMG
                     ETPLMYVAIAGDTARAQALLARGAQVNKLGWTPLHYAVSKGQLDMAKLLLARDAMPNA
                     PAPDGITPLMMAAYAGSRPAVQLLLTAGADPLVQDAKNQDAADWARQGKAYALSDELH
                     TLIAQRLQTRRQTGQDAPVQTLPAQAPAPAAPVPQAEAPAKTEPAEGGTNYVKGLSNV
                     QLNKY"
     gene            complement(3352..>3673)
                     /locus_tag="VGC69_00675"
     CDS             complement(3352..>3673)
                     /locus_tag="VGC69_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0293826.1"
                     /translation="RPLDTPLPAAWQARVGTHWTLGNESPESVTWLLDPKQDYTLDVL
                     PGLPGYLMWCGGQLLQPLSDTRAGMTVRVPVNNGRDLNELVVDAQDGQETLWLGGSRL
                     VRRA"
BASE COUNT          590 a         1351 c         1213 g          519 t
ORIGIN      
        1 gagggcgtgg acggcgcggg caagagcacg catgccggct ggctggtcga ggccatccgc
       61 gcggccggcc acaccgtgct ggccacgcgc gagccgggcg gcacgcccat cggtgaaaag
      121 ctgcgcgagc tggtgctgca cgaacccatg cggctcgaga ccgaaaccct catcatgttc
      181 gcggcgcgct gcgagcacct gcgcaccgtg atcgagcccg ccctggcgcg cggcgagtgg
      241 gtcgtgtgcg accgtttcac cgatgccagc tatgcctacc agggcggcgg ccgggaactg
      301 ggcgccgccc ccgtggccgc gctcgaagcc tggatcggcg cgccccagcc cgaccgcacc
      361 tggctgttcg acgtgccgct ggcggtcgcg cgcgcgcgcc tggccgccag ccgcgacccg
      421 gaccgcttcg agcgcgaggc cggcgccttc ttcgaacgcg tgcgggcggc ctaccaggcg
      481 cgcgcccagg cccatcccga gcgcgtgcgg gtcatcgacg gcagccagcc catggccgac
      541 gtgcgcgccc ggctcgaggt cgacctgcgc gaactgctgc ggcaaggacc cggcgcatga
      601 gcggctttct gccctggcac ctggagacgg cccgcgcctg gctggcccag cgcgaccgtt
      661 tcgcccatgc ctggctgctg cacggcgagg ccggcatcgg caagcaggac ttcgccgtcg
      721 ccgcggccgc cagcctcctg tgcgaaaccc cgcagcaggg cctggcctgc ggccattgca
      781 tcgcttgcgg ctggttcgcc tcgggcaacc atcccgacct gcgccgcatc cgtcccgagg
      841 ccgtggcgct gaccgaaggc gcgcaagcca gcgaatcgga cgaggcggaa acggcggcgg
      901 cgggcagcaa gcgcgcgccg tccaaggaca tccgcatcga ccagatacgc agcctcgagc
      961 cctggttcaa caccgccacc caccgcggcg gctggcgcgt ggccttgctc tatccggccc
     1021 aggccttgaa caccatcgcg gccaatgcct tgctcaaggt gctggaagag ccgccggcgc
     1081 acaccgtgtt cctgctggtg agcgatgcgc ccgaccggct cctgcccacg ctggtgtcgc
     1141 gctgccggcg cctgccgctc gcggcgccgc ccgccgagac cgccttgcaa tggctggccg
     1201 ggcagggcgt caagcagccg gccgactggc tggcggcggc cggcggcacg ccgctgtcgg
     1261 ccttgcgcct gtcgcaaaag caggacgaac cttgccccgg ctggctgggc cagttgctga
     1321 cgccattggc ccagggacac cggcccgacg tgggcagcct gaccgaaaca ctggaaaaag
     1381 tggcggcgcc cgagtggctg gacgccctgc agcgtgtctt caccgacctg atgctggcgg
     1441 ccaacggcct gtcggtgcgg tatttcccgg cgctggccga attgacggcc cacaccgcgg
     1501 cgcgcgtgca aatcgcccgc ctggccgata ccgcgcactg gctggtcagc cagcgcgccg
     1561 tggccgggca cccgctcaac cccaagctct tcatccatgc cacgctgcag cgggtggcgc
     1621 ttgccctagc ctgagatttt catgtacgtc gattcgcact gccacctcaa ctttcccgac
     1681 ctggcgcagg acctgcccgg cgtgctcgag cgcatgcgcc agaaccaggt cacccacgcg
     1741 ctcctggcca gcgtcaccat gccgtcctgg ccgggcctaa tggagatcgt ggagtccacg
     1801 cccaacctgt ggggctcggt cggcgtgcat cccgacgaag aaaacacccc cgagcccgat
     1861 gtggacgagc tggtgcggct ggcccggcac cccaaggtgg tggccatcgg cgaaaccggg
     1921 ctggactact accgcctgag cgagccgctg gactggcagc gcgagcgctt tcgccgccac
     1981 atccgcgccg ccaggcaggc gaagctgccg ctcatcatcc acacccgcaa ctcggccgcc
     2041 gacaccgtgc gcatcctggc cgaggagggc gccgaggaaa tcggcggcgt gatgcattgc
     2101 tttaccgaaa cctgggagat cgcccagcag gcgctggacc tgaacttcta tatttcgatg
     2161 tcgggcatcg tcaccttcaa gaatgcccag gtggtgcagg acgtggccac ccgcacgccg
     2221 ctcgaccgcc tgctgatcga gaccgactcg ccttacttgg cgcccgtgcc gtaccggggc
     2281 aagctcaacg acccggccaa ggtggtgtac gtcgctgaaa aagtggccga cctgaaaggc
     2341 ctgggctcag ccgacatagc gaaacaaacc tccgaaaatt tcttcaaatt attcaataag
     2401 atagaaaaat aatctcatct ggtttatttg aagtaattta tttaaagtgt tttatctgaa
     2461 gcaagttgcg aggttcgtgc catgatgacc cagcccgctt tccgccgccg cctgcgtgcc
     2521 gcgctggccg ccttgtgcct ggccgcgccg atctgggcgg gttcggtcgc gcacgcccag
     2581 gcgacgccgc ccgactggtg ggtggacatc gaaaacgacc gcgccgacaa gatccgcagc
     2641 gaactcgccc agggcgtcga tcccaacctg aaggcgtcca acggccagcc cgcgctgctg
     2701 cgcgccgtgg cttccgattc gtggaaagcc ttcgacgcgc tgctcgccga cccgcgcacc
     2761 gacgtcaacg ccgagaaccc catgggcgag actccgctca tgtacgtggc catcgccggc
     2821 gacaccgcgc gcgcccaggc gctgctggcg cgcggcgccc aggtcaacaa gctgggctgg
     2881 acgccgctac attacgccgt ctccaagggc cagctcgaca tggccaagct gctgctggcg
     2941 cgcgatgcca tgcccaatgc gccggcgccc gacggcatca cgccgctgat gatggcggcc
     3001 tacgccggca gccgcccggc cgtgcaactg ctgctgaccg ccggcgccga ccccctggtg
     3061 caggacgcca agaaccagga cgccgccgat tgggccaggc agggcaaggc ctatgcgctc
     3121 tccgacgagt tgcacaccct gatcgcgcag cgcctgcaga cccgccggca aaccggccag
     3181 gatgcgccgg tgcagacctt gccggcccag gcgccggcgc cggcagcgcc ggttccccaa
     3241 gccgaggcgc ccgcgaaaac cgagcccgcc gaaggcggga cgaactacgt caagggcctc
     3301 tcgaacgtgc agttgaacaa gtattgaccg tccgggcggc agggcgccgg ctcaggcccg
     3361 gcgcaccagg cgcgagccgc ccagccacag cgtctcctgg ccgtcctgcg cgtcgacgac
     3421 cagctcgttc aggtcccggc cgttattcac gggcacgcgc accgtcatgc cggcgcgcgt
     3481 gtccgacaag ggctgcagca actgcccgcc gcaccacatg aggtagcccg gcagtccggg
     3541 cagcacgtcg agggtgtagt cctgcttcgg gtccagcagc caggtcaccg actcgggcga
     3601 ttcgttcccg agcgtccagt gcgtgcccac gcgcgcctgc caggccgcgg gcagcggcgt
     3661 gtccaggggc cgc
//
LOCUS       DASWPO010000017         5439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_182054, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000017 DASWPO010000000
VERSION     DASWPO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182054"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGC69_00680"
     CDS             complement(<1..264)
                     /locus_tag="VGC69_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY0293827.1"
                     /translation="MRSLFRLLDRFAVRSIGLSALLCLAALGSPAPARADAGAAPAAK
                     TVLVVGDSLSAEYGIARGTGWVPLLGRRLAERYPNYRVINASIS"
     gene            263..934
                     /locus_tag="VGC69_00685"
     CDS             263..934
                     /locus_tag="VGC69_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0293828.1"
                     /translation="MESINSIEVRGLGKRVADAGGELAILDGIEFTVKAGSAVAITGS
                     SGSGKSTLLGLLAGLDAPSSGSVTLAGQDLFSLDEDARAQLRARHVGFVFQSFQLLPN
                     LTALENVMLPLELAGKPAREAAEAMLERVGLSARLHHYPRTLSGGEQQRVSLARAFVA
                     QPSLFFADEPTGSLDAATGEKIIDLMFAMHKEQGATLILVTHDPQLAARCGQQLVLSG
                     GKLMP"
     gene            complement(1046..1924)
                     /locus_tag="VGC69_00690"
     CDS             complement(1046..1924)
                     /locus_tag="VGC69_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0293829.1"
                     /translation="MWGIGLLFASMWVLPFLDASGKWLVGHGVAVIFLSWMRYVVHLL
                     VMLAAMVPGRGLRVLRSRRPRDQILRGASMLGGTLMFFSTLHYLPQSEATAINFLSPL
                     ILLLVAPWVLREPSRPSRWIAAGCAFAGVLVIIRPGSGLDPVGVTLGLLSAFCFAAQY
                     IFTRRVASDDPYTSIIWSGAVGALVLTPMLLLPSADDWSALREIGPGTWLLLCSTGLT
                     GALGHLLQIAAYRNAPASALAPFTYFSVITATVVGWLVTGHFPDAVTWIGIGVIFVSG
                     VGTGIYEWRMPRKAQA"
     gene            2095..3006
                     /locus_tag="VGC69_00695"
     CDS             2095..3006
                     /locus_tag="VGC69_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0293830.1"
                     /translation="MIDLRNIETFFWVATLGGFRAAAEKLNATQPAISQRIASLESDL
                     GVRLLERDTRGVSLTAKGAELLSHAERMLQMRQDMQVAARARSVMSGTLRLGVSETIV
                     HTWLPRLMERLSDAYPALMVEIQVDTTTSLKTALGAHQIDLAFLLGPVDDPRIENLYL
                     CQYPLHWIAGPALKVGRQPVSLQRLSQWPIITYSSATDPHRAVREMMQQAGIKQPRMY
                     GSSAVSVIVRMVQDGIGTGVLATVFLSKELERGELRLLNVKAPPLPPLHYTACWMQGP
                     DSHMARMVAEVAQRIAREEDAKHQVEP"
     gene            3129..3923
                     /locus_tag="VGC69_00700"
     CDS             3129..3923
                     /locus_tag="VGC69_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEY0293831.1"
                     /translation="MASNALADLAYQARLDARSGQYTGPTANLAPGRVQANLAILPRA
                     LAADFLHFCQRNPKPCPLLAMSEPGCPALPALGRDIDIRTDIPRYRVWRHGELVAEPL
                     DVREFWRDDLVSFLIGCSFSFEEAMIENGLPVRHIEQGCNVPMYRTRVPTNPAGAFNG
                     PLVVSMRPLKAADAIRAIQVTSRFPSVHGAPVHLGNPAEIGIADIGKPDYGDPVEIRE
                     GEIPVFWACGVTPQSVVASVKPEFCITHAPGHMLVTDLLNSRMAVL"
     gene            4076..5326
                     /locus_tag="VGC69_00705"
     CDS             4076..5326
                     /locus_tag="VGC69_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0293832.1"
                     /translation="MNKKFLAGALASLALAATLPAHAADKTIRIGAIYPLTGALASTG
                     DEMKQAIDVALDIINNPHPDLAGIPLAAGSGLPNLGGAKLEVVYDDSQGKPEVALADA
                     QRLIDQEHVVALTGAYQSAVTKTASRIAEQRGIPYLNGESSSPDLTERDYKWFFRTTP
                     NDETFVENMMQFLDGIKAVPTAKIAVVYENTDFGVNTYKAIEKFAKQYHREIVANIAY
                     SANSASVTAEVQKLAGAKPDVAIFASYVSDAMLFIRTMKEAKYAPPVFMANDAGFIDT
                     RFIQEMGPQVQGVLTRDVWGTDVAGAKPALKTVNDLFKAKYGKDLNGNNARELQAILV
                     LADAINRAGSTDPEAIRKAIAATDLSAEQVVMPWAGVKFDDKGQNTKGAGLILQMKGP
                     GYQTVWPEAYRTNKALPELPFKWQ"
BASE COUNT         1025 a         1885 c         1699 g          830 t
ORIGIN      
        1 gctgatgctg gcgttgatga cacggtaatt agggtatcgt tcggctagcc ttcggcccag
       61 cagcggcacc cagcccgtgc cgcgcgcaat gccgtactcg gcggacaggc tgtcgcccac
      121 caccaacacg gtcttcgcgg ccggcgcagc gccggcatcg gcccgcgcgg gagctggcga
      181 ccccagcgcg gccaggcaga gcagggcgga caggcctatg gaccggacgg caaaacggtc
      241 aagcaaacgg aataatgagc gcatggaaag catcaattcg atcgaggtac gaggactggg
      301 taagcgcgtg gccgacgccg gcggcgagct agccatattg gatggtatcg aattcacagt
      361 aaaggccggt agcgccgtgg ccattacagg cagttccggt tcgggcaaat ccaccttgct
      421 gggcttgctt gccggcctgg acgcgcccag ttccggcagc gtcacgctgg ccgggcagga
      481 tctgttctcc ctggacgagg acgcccgcgc ccagctgcgg gcccgccatg tcggcttcgt
      541 gttccagtcc ttccagctgc tgcccaacct gaccgcgctc gaaaacgtca tgctgccgct
      601 ggaactcgcc ggcaagccgg cgcgcgaagc cgccgaagcc atgctcgaac gcgtgggcct
      661 gagcgcgcgc ctgcaccatt atccccgcac gctctccggc ggcgagcagc agcgcgtttc
      721 cctggcccgg gccttcgtcg cgcaacccag cctgttcttc gccgatgagc ccaccggcag
      781 cctggacgcc gccacggggg aaaagatcat cgatttgatg ttcgccatgc acaaggaaca
      841 aggcgcgacc ttgatcctgg tcacgcatga cccgcaactg gccgcgcggt gcgggcagca
      901 gttggtcttg tccggcggca aattgatgcc ttgagccgcg gcccccggag gcatccgacg
      961 cacagcccct ttccgggggc gcgcaccggc atgccgcagg gcagccgtgg cggctcaaga
     1021 acgcaagccg tggcggccca ggaactcaag cctgcgcctt ccggggcatc cgccactcgt
     1081 agatccccgt ccccacgccg ctgacgaaga tcaccccgat cccgatccag gtcaccgcgt
     1141 ccggaaaatg cccggtgacg agccatccca ccaccgtggc cgtgatcacc gaaaaatacg
     1201 taaaaggcgc cagggccgac gccggcgcat tgcggtaggc ggcgatctgc agcagatgcc
     1261 ccagggcccc cgtcaacccc gtcgagcaca gcagcagcca ggtccctggc ccgatctcgc
     1321 gcaaggccga ccaatcgtcg gccgaaggca gcagcagcat aggcgtcagc accaaggcgc
     1381 ccacggcccc gctccagatg atcgaggtat acggatcgtc gctggccacg cggcgggtga
     1441 agatatattg ggcggcaaag cagaatgcgc tcagcagccc cagcgtgacg cccaccgggt
     1501 ccagcccgct gcccggccgg atgatgacca gcacgcccgc gaacgcgcag cccgccgcga
     1561 tccagcgcga cggacgcgaa ggctcccgca atacccaggg cgcgaccagc agcaggatca
     1621 gcggcgacag gaaattgatg gccgtcgctt ccgactgcgg cagatagtgc agggtgctga
     1681 agaacatcag cgtgccgccc agcatgctgg ccccgcgcag gatctggtcg cgcgggcggc
     1741 ggctgcgcag cacccgcaac ccgcggccgg gcaccatggc ggccagcatg acgagcagat
     1801 gcacgacata gcgcatccac gacaggaaga tcaccgcgac gccatggccc acgagccatt
     1861 tgcccgaggc gtccagaaag ggcaggaccc acatcgacgc gaacagcagt ccgatgcccc
     1921 acatcaggcg ggtaggcggg gtgacagcgc gtgggcttgt tttttgatcc ggagcctggc
     1981 ttcccgcagt cgccaggctg ggctgggccc gttgcatgga gtctcctcga aacaggccgc
     2041 catgatacgc ccacgccaca tgggctgcgg gccctggggt atgcttgccg tgccatgatc
     2101 gacctgcgca atatcgagac ctttttctgg gtggccacgc tgggcggctt tcgcgcggcc
     2161 gccgaaaagc tcaacgccac gcagccggcc atctcgcaac gcatcgcttc cctcgaatcg
     2221 gacctggggg tgcgcctcct ggagcgcgat acgcgcggcg tatcgctcac ggccaagggc
     2281 gccgaactgc tctcgcacgc cgagcgcatg ctgcagatgc gccaggacat gcaggtcgcg
     2341 gcgcgggccc gcagcgtcat gagcggcacc ttgcgcctgg gggtttccga gaccatcgtg
     2401 catacctggc tgccgcgcct gatggagcgg ctctccgacg cctatccggc gctcatggtg
     2461 gagatccagg tcgacaccac cacctcgctc aagacggcgc tgggcgcgca ccagatcgac
     2521 ctggcctttc tgctcggccc ggtggacgac ccgcgtatcg agaacctgta cctgtgccag
     2581 tacccgctgc attggatcgc ggggccggcg ctcaaggtgg ggcgccagcc cgtctcgctg
     2641 cagcgcctgt cgcaatggcc catcatcacc tattcgtcgg ccaccgatcc gcatcgcgcg
     2701 gtgcgcgaga tgatgcagca ggccggcatc aagcagccgc gcatgtacgg cagctcggcg
     2761 gtgagcgtca tcgtgcgcat ggtgcaggac ggcatcggca ccggcgtgct ggccacggtg
     2821 ttcctgtcca aggagctgga gcgcggcgaa ttgcggctgc tcaatgtcaa ggcgccgccc
     2881 ttgccgccgc tgcactacac cgcctgctgg atgcaagggc ccgacagcca catggcccgc
     2941 atggtggccg aggtggcgca acgcattgcc cgcgaagaag acgccaagca tcaggtagaa
     3001 ccctgataaa aaattttgat aagagcctat cgcaacatgt gattggacgc gttgcgccga
     3061 ttccgttgca ataagcccca tagccgatca acggcaatga atgtaaagag agttcaaggg
     3121 gtttttcgat ggcgtcgaac gcactcgccg atcttgccta ccaggcgcgc ctggatgcac
     3181 gcagcggcca atacaccggt cccacggcca acctggcgcc aggccgcgtg caggccaacc
     3241 tggccattct gccgcgcgcg ctggcggccg atttcctgca tttctgccag cgcaatccca
     3301 agccttgtcc cttgctggcc atgtccgaac cgggctgccc ggcattgccc gcgctgggcc
     3361 gcgacatcga catccgcacc gacatcccgc gctaccgggt ctggcgccac ggcgagctgg
     3421 tagccgagcc gctggacgtg cgcgaattct ggcgcgacga cctggtgtcc ttcctgatcg
     3481 gctgctcttt ctcgttcgaa gaagccatga tcgagaacgg cctgccggtg cggcatatcg
     3541 agcagggctg caatgtgccc atgtaccgca cccgggtgcc caccaacccg gccggcgcct
     3601 tcaacggccc gctggtcgtg tcgatgcgtc cgctgaaggc ggccgatgcc atccgggcca
     3661 tccaggtcac ttcgcgtttt ccctcggtgc acggcgcgcc ggtgcacctg ggcaatccgg
     3721 ccgagatcgg catcgccgat atcggcaagc ccgactacgg cgatccggtc gagatccgcg
     3781 agggagaaat cccggttttc tgggcctgcg gcgtcacgcc gcagtcggtg gtggcctcgg
     3841 taaaaccgga gttctgcatc acgcatgcgc cgggccatat gctggtaacc gatctgctca
     3901 acagccggat ggccgtgctt taatcatcgg gtcgatccgt cgcggatcaa cgtgatcaca
     3961 tttgcttttc actggtttca cacacaggcc gcactgcttt ccacggccat ttcgcagtct
     4021 atttgtccat agccagtcca ttgtttccac aaccggattt tccaggagtc gacgcatgaa
     4081 caagaaattc ctcgctggcg cattggccag cctggccctg gccgcaacgc tgcccgcgca
     4141 cgccgcggac aagaccatcc gcatcggcgc gatctatccg ctgaccggcg cgctggcctc
     4201 gaccggtgac gagatgaagc aggccatcga cgtggccctc gacatcatca acaacccgca
     4261 tccggatctt gccggcatcc cgctggccgc gggcagcggc ctgcccaacc tgggcggcgc
     4321 caagctcgag gtcgtgtacg acgactccca gggcaagccc gaagtggcct tggccgacgc
     4381 ccagcgcctc atcgaccagg agcacgtcgt ggccctgacc ggcgcctacc agtcggccgt
     4441 caccaagacc gccagccgca tcgccgagca gcgcggcatt ccctacctga acggcgagtc
     4501 gagcagcccg gacctcaccg agcgcgacta caagtggttc ttccgcacca cgcccaacga
     4561 cgaaaccttc gtcgagaaca tgatgcagtt cctcgacggc atcaaggccg tgcccaccgc
     4621 caagatcgcc gtggtgtatg aaaacaccga cttcggcgtg aacacctaca aggccatcga
     4681 gaagttcgcc aagcagtacc accgcgagat cgtcgccaac atcgcctaca gcgccaactc
     4741 ggcctcggtg accgccgagg tgcagaagct ggccggcgcc aagccggacg tggccatctt
     4801 cgccagctac gtgtccgacg ccatgctgtt catccgcacg atgaaagagg ccaagtacgc
     4861 gccgcccgtg ttcatggcca acgacgccgg cttcatcgac acgcgcttca tccaggaaat
     4921 gggcccgcag gtgcagggcg tgctcacccg cgacgtgtgg ggcaccgacg tggccggcgc
     4981 caagcccgcg ttgaagaccg tcaacgacct gttcaaggcc aagtacggca aggacctgaa
     5041 cggcaacaac gcccgcgagc tgcaggccat cctggtgctg gccgacgcga tcaaccgtgc
     5101 cggctccacc gatcccgagg ccatccgcaa ggccatcgcc gccaccgacc tgagcgcgga
     5161 acaggtcgtc atgccctggg ctggcgtgaa gttcgacgac aagggccaga acaccaaggg
     5221 cgccggcctg atcctgcaga tgaagggtcc cggctaccag accgtctggc ctgaagccta
     5281 ccgcaccaac aaggcgctgc ccgaactgcc cttcaagtgg cagtaaaccg gtgaaggcgg
     5341 gcggtcctgc gccgggtggc gcaagcccgc ccgccttgcg aaaagcggca aacaagaacg
     5401 ggcaacaggc ccgctgcaag ggataacaaa tgctagaag
//
LOCUS       DASWPO010000018        13200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2145506, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000018 DASWPO010000000
VERSION     DASWPO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13200
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145506"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VGC69_00710"
     CDS             <1..1071
                     /locus_tag="VGC69_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0293833.1"
                     /translation="IAYSHGKKSVCLDLARPDGHAAFCKLLATADVLVHNLAPKAARK
                     LRLSREDCAAINPRLIYCHIRGYAAGPQADDLASNPVAEAATGVMESNRIDGRPSRLG
                     PSYHDQFAGAYAVIGVLAAMLQAKAMDQHGEQVEVGLYETGLHVAARDLVGVQLKTQL
                     LGRPEREPNGEFSMPGYGAYLTADKRWIYLLMLTDAHWLKFCQAMHLPQAHDEHLALL
                     RNRKKVREQVEEAVRAAIAAHTFDEVQARLKAAGVGCTEVLPLERVLDAPQARHPGKL
                     RSVRYRELDFDIPEFPRLHATDTGTPQPPPELGEHTVAVLRSAGLSPQACDALLASGA
                     ALAVKQGDFAWAPVRSQDARTP"
     gene            1146..2129
                     /locus_tag="VGC69_00715"
     CDS             1146..2129
                     /locus_tag="VGC69_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0293834.1"
                     /translation="MLASWKHRFARLSWTLLALALALPLHAAEPEGPIRLVVGFPPGG
                     GADYVARQVARYLTQELSQSVIVENKPGANGIIAATEVAHAAPDGHTLLLGVTATQSI
                     NPNLYSKLPYDPIRDFTPISEVGSTPLVLVVSPSMPVKSVPDFIHYVKQHNGPVYFAS
                     AGNGNITHMAAELFILSTDTRNLSHIPYKGSSPAITDLLGGRVSAYFDTLPSSMPFIR
                     SGQLHALGVTSARRAQALPDLPTIQEQGVPGYEATAWFGLFGPSKLDPDTTQRLYKAL
                     QHAFAQPEAQQLMTNQGLEVDIDTPQHFSAMLKADVARWHTVTTQAHIHLD"
     gene            2275..4590
                     /locus_tag="VGC69_00720"
     CDS             2275..4590
                     /locus_tag="VGC69_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY0293835.1"
                     /translation="MTPSPSGLSSTEAGLRLKRDGANAIVDVKPHPLWRAVKKLWAPV
                     PWMLEAAIVLQVGLGDYISACIVLFLLLFNAGLGLFQEGQAQATVDALKSRLALVSPV
                     LRDGAWATVPAAELVAGDIVKLSLGSVVAADVRLISGSVLLDQSMLTGESAATESGAD
                     SQAFAGALVRRGEAVAEVVATGERTKFGRTAELVRTAKVESSQQKTVLRVVRNLVLFN
                     GAVTILLGAYALLVSMPLDEIVPLLLVAILSSIPVALPSMFTLAAAVGARSLASRGVL
                     PTRLSAVDEAAGIDILCSDKTGTLTRNELAVMSVRPMPPFDEAHVLAFAALASSDGGQ
                     DPVDSAIRGASAGKPIADQPALVAFVPFDPALKRAEATLRHANGEERRVLKGAFATIA
                     ALSRPCPSAAAIVAEMEGSGFRVLAVAEGSANDLALAGLIALSDPPRSDSAALVTELR
                     DMGVRTVMVTGDARKTAETVAASVGISGTVWAATPLPGDLDVQPYSIFAGVLPEDKYQ
                     LVKSLQQRGHIVGMCGDGANDAPALRQAQMGIAVSTATDVAKSAAGVVLTEPGLGGIV
                     ALVKEGRTTFQRILTYTLRSIIHKTVQVLFLAAGLVMTGHAILTPTLMVLMMVTGDFL
                     AMSSSTDNVRPSPRPNIWRIRNLTLAGIAMGMIDLLFCVACLATGKFALGLGTDALRT
                     VAVVTLVFSGQAVFYVARERQHLWSSPPGRWLVVSSIADVAIIGVLAINGILMAALPL
                     DMIAGFLAAAVILAFILDTLKQVLFRRLAIV"
     gene            complement(4639..5289)
                     /locus_tag="VGC69_00725"
     CDS             complement(4639..5289)
                     /locus_tag="VGC69_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0293836.1"
                     /translation="MKTDTQIQRDVLEELKWEPSLPAAGIGVEVNDGVVTLAGHVGSY
                     AEKLAAQRAARRVAGVKTLAVEVDIRLIDTHTRNDTEIASAVDNALRWSTVIPDGCIN
                     VTVEKGWITLDGQVDWDYERQSATKAVRYLMGVTGVSNQIEIKPKVSVSAVKADIEAA
                     LKRRFHDETAAISVTVAGNQVTLTGRVHSWPERKLAENAAWTAPGVYHVDDQIEVV"
     gene            complement(5406..5957)
                     /locus_tag="VGC69_00730"
     CDS             complement(5406..5957)
                     /locus_tag="VGC69_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY0293837.1"
                     /translation="MTQTIYDIPVKSIDGAALTLAAYRGKVLLIVNVASKCGLTPQYE
                     GLETLYEDKRGQGLEVLGFPANNFKGQEPGSDEEIRQFCSTTYDVTFPLFSKISVVGD
                     DQHSLYQALTAAQPDATGDGPFRERLKGYGMAPNPAPQVLWNFEKFLVGRDGRVVGRF
                     APDVAADDPRLLQAIDAALLGQA"
     gene            complement(6171..7649)
                     /locus_tag="VGC69_00735"
     CDS             complement(6171..7649)
                     /locus_tag="VGC69_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0293838.1"
                     /translation="MSPHPILQLIPSCLAAGLNLLLVLLGMPNRLGLRTLGRCAAFAL
                     LTVAMLHDDIVPTQVLYHAAEPEIRFIWGMLEVAWWMLAASTVTAVVRTYYTLGGRLR
                     ERRFMLDVIGTLLYLSAVLAIITDVFHIPLRGVLATSGVLAIVLGLALQSTLSDLFSG
                     LLINATAPYRVGDSISLDGGTEGTVVEITWRATHLARANRDLIVVPNSTIAKSRIVNA
                     SVPSGPHTTVARFQAPSQYRPSDVVQALQLAANTCVGIAAYPKPVIVTKSIGFEATDY
                     EVSFFALARWQAEDVLNSFYDAAHRHLESIHTLIAPIVPARGIGTQTLEYQLVEGVRV
                     FGLLSKDDRVKLAAALVRRELTEGDQLLAAGELPRAITIVGYGILAATMNTGDPSSDI
                     LRFGPREYLGESGPIMGVPIKLAIVARTNAIVYDLPGEAVAGLLQAHPDVAHALSARL
                     ADREREGQALMQPQAEIPVTRHGLARWIAQCIQSLHRGRPLP"
     gene            complement(7840..7916)
                     /locus_tag="VGC69_00740"
     tRNA            complement(7840..7916)
                     /locus_tag="VGC69_00740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7880..7882),aa:Pro,seq:tgg)
     gene            8053..8574
                     /locus_tag="VGC69_00745"
     CDS             8053..8574
                     /locus_tag="VGC69_00745"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0293839.1"
                     /translation="MLTPDAARMLADYKKWANQETYAIVLALPPEEIYKERVSLFKNI
                     ARTLNHIYVVDLIWQAHVEGRPHGIAALNTATHEEIGALWEAQQAMDDWYIAWAAQLT
                     EAGLAEDKRYELIGGNTGSMSCAEILLHAVNHGSYHRGFVSDLLTQIPAKRPTVDLPV
                     YKRVRAAAAAAAA"
     gene            8675..9049
                     /locus_tag="VGC69_00750"
     CDS             8675..9049
                     /locus_tag="VGC69_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293840.1"
                     /translation="MVRKLTLSILGTAALAIAGHAYAAPGMASNGVLVGDKGMTLYTF
                     DKDAANTGKSVCNGDCATKWPPFAAPASAQADGAWTVVTRDDGSKQWAYQGKPVYYWF
                     KDAKPGDKTGDGVVGVWHIIKP"
     gene            9058..9582
                     /locus_tag="VGC69_00755"
     CDS             9058..9582
                     /locus_tag="VGC69_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0293841.1"
                     /translation="MPSAEGVDQILAHIPALRRYARLLAGDAASADDLVQDTLERACT
                     KWSLWKPGTALRAWLMTLMHNLYLNRVRDWGRDDNHDIWDEASGPTHEPLAHAPELLD
                     LERALARLSPMLRTVLLLVGVEEYTYAEAAEILQIPIGTVMSRLHRAREAVRLSMNGA
                     ADAAAPTPLHLVKR"
     gene            9579..10430
                     /locus_tag="VGC69_00760"
     CDS             9579..10430
                     /locus_tag="VGC69_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY0293842.1"
                     /translation="MTDTDALVTDDELHAFVDGQLDAARIAAVLQHLQQHPEAAVRAA
                     QWHAQRLALRRLRRAADPGPTPDALTQTVLRRRRSRWNPWTQAAAAMLLVAAGAAGTQ
                     AWQVATTPAAATVAATAPQFVQDATTAYAVYAPEVRHPVEVTAQEEAHLVQWLSRRLG
                     RPLVAPDLQGQGFHLLGGRLLPGEPAGTAAPASAASDAGASGNPAARAQFMYEDTHGR
                     RVTLYVAVFAPDVAPRETAFRSVSSASGVAFYWVDNGYGYALNGDLPAAELQHLAGGV
                     YDQLFPR"
     gene            complement(10473..11984)
                     /locus_tag="VGC69_00765"
     CDS             complement(10473..11984)
                     /locus_tag="VGC69_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0293843.1"
                     /translation="MLRRLTLTQRLALVFALLLAVCSGTSAWLQIRSNRMHALEVVQA
                     LSRDLAEHIARDGQLADGSGLKPDAVRQLFRQLMVVNPSVEVYLLDQDGRITGHAAPA
                     NRIMRDHVDLGPVRALLAGAPLPVLGDDPRSERGRKVFSAAPLRVDGREMGYVYVVLL
                     SEAHDRLDASEATSTALDVGLWSIGLVALLCLVAGLAAFGLITRPLRRLTDTVKGFDI
                     DGAPAALPPAAEPPGAGGDEIAVLDGAFRQMAGRLGEQWRALTRQDQDRRELIANISH
                     DLRTPLTSIHGYLETLVLKDATLAPSERRRYLGIALDQSGKVSRLAQSLFELARLEYG
                     AVRPEIEPFSLVDLVQDVFEKFELPAQSRQIALRARFTERVPPVRADLGMIERVLTNL
                     LDNALRYVPAGGEIEVALAVRDAFVDVTVSDTGPGIPAELRAGLFRQPFEVGGARRGG
                     GLGLRIVHRILALHGCGIALIDLPGRGAALRFSLPAVEGRRPAGLDARPSGTA"
     gene            complement(11987..12706)
                     /locus_tag="VGC69_00770"
     CDS             complement(11987..12706)
                     /locus_tag="VGC69_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0293844.1"
                     /translation="MDQPRRVLVVEDDMQIADLLALNLRDEGYDVTHCAHGEDGLRQL
                     EQGGWDALILDLMLPGIDGLEICRRARAMPRYIPIIILSARASEVHRILGLELGADDY
                     LAKPFSVLELIARVKALLRRVDALSQNARIEAGSLSVGGLFIDPIAREALLAGRRLDL
                     TPREFDLLYFFARQPGKVFSRMDLLNAVWGYSHDGYEHTVNTHINRLRAKIEVDPAQP
                     EHILTVWGRGYKFAVPEGKPS"
     gene            12866..>13200
                     /locus_tag="VGC69_00775"
     CDS             12866..>13200
                     /locus_tag="VGC69_00775"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY0293845.1"
                     /translation="MLTRRQFFYLGGGALLAAGVLPRILAGTASAAPEAADGPFEVTH
                     TEAEWHALLTPAQYAVLRDSGTERPYSSPLDDEHRAGTFACAGCGLPLFSSATKFDSR
                     TGWPSFWAA"
BASE COUNT         2253 a         4627 c         4230 g         2090 t
ORIGIN      
        1 atcgcctaca gccacggcaa aaaaagcgtc tgcctggacc tggcccgccc ggacggccat
       61 gcggcctttt gcaaactgct ggccacggcc gacgtgctgg tccacaacct ggcgcccaag
      121 gccgcgcgca agctgcgcct gtcgcgggaa gactgcgccg ccatcaatcc gcgcctgatc
      181 tactgccaca tccgcggcta cgccgccggc ccccaggccg atgacctcgc atcgaatccc
      241 gtggcggagg ccgccacggg cgtcatggaa agcaatcgca tcgacggccg tcccagccgg
      301 ctgggaccgt cctatcacga tcagttcgcc ggcgcctatg ccgtcatcgg cgtcctagcg
      361 gccatgctgc aggccaaagc catggaccag cacggcgagc aagtcgaggt ggggctctac
      421 gagaccgggc tgcacgtcgc cgcacgcgac ctggtcggcg tgcaactcaa gacgcaactg
      481 ctgggacgtc cggagcgcga gcccaacggc gagttcagca tgccgggcta cggcgcctat
      541 ctcaccgcgg acaagcgctg gatctacctg ctcatgctca ccgacgcgca ttggctgaag
      601 ttctgccagg ccatgcacct gccgcaagcc cacgacgaac acctggcctt gctgcgcaac
      661 cgcaagaagg tgcgggaaca ggtcgaggag gccgtgcgcg cagccatcgc ggcgcacacc
      721 ttcgacgagg tgcaggcccg cttgaaggcg gccggcgtgg gatgcaccga agtcctgccc
      781 ctggagcgcg tactggacgc gccccaggcg cgccacccgg gcaagcttcg atccgtgcgc
      841 taccgcgaac tcgatttcga catcccggaa tttccccggc tgcatgcaac cgacactgga
      901 acgccacagc cgccgcccga attgggcgag cacaccgtgg cagtcctgcg ctcggcgggc
      961 ctgtcgcccc aagcgtgcga cgcgctgctg gccagcggcg cggccctggc cgtcaagcaa
     1021 ggagacttcg cctgggcgcc cgtacggagc caggatgccc gcacgccctg agaacaccca
     1081 atagctgcac cccacactga caccacggca aggcccgaca gaggccatca tcggaggaga
     1141 ctgcaatgct tgcttcatgg aaacaccgct tcgcgcgcct gagctggacc ctgttggcct
     1201 tggcgctggc cctgcccctg catgcggccg agcccgaagg ccccatacgc ctggtggtgg
     1261 gctttccgcc cggcggcggc gcggattacg tggcccggca agtcgcccgc tacctgaccc
     1321 aggagctcag ccagagcgtc atcgtcgaaa acaagcccgg cgccaacggc atcatcgccg
     1381 ccaccgaagt ggcccatgcc gccccggacg gccataccct gctgctgggc gtcaccgcca
     1441 cgcagtcgat caaccccaat ctctactcca agctgcccta cgacccgatc cgcgacttca
     1501 cgcccatcag cgaagtcggc tccacgcccc tggtgctcgt cgtatcgcct tccatgcccg
     1561 tcaagtccgt gccggacttc atccactacg tgaaacagca caatggcccg gtgtatttcg
     1621 cgtcggcggg caacggcaac atcacccata tggcggccga actgttcatt ctttcgaccg
     1681 acacccgcaa cctgtcgcac atcccctaca aaggcagcag cccggccatc accgatctgc
     1741 tcggcggccg cgtttccgcc tacttcgaca ccttgccgtc atccatgcct ttcatacgga
     1801 gcggccaact gcatgccctg ggcgtgacca gcgcccgccg cgcccaggcg ctgccggatc
     1861 tgccgacgat ccaggagcaa ggcgtccccg gctacgaggc gaccgcctgg ttcggcctgt
     1921 tcggcccgag caagctcgat cccgacacca cacagcgcct gtacaaggcg ctgcaacacg
     1981 ccttcgccca acccgaggcg cagcaactca tgaccaacca gggactggaa gtcgacatcg
     2041 acacgccgca gcacttcagc gccatgctca aggccgacgt cgcccgctgg cataccgtca
     2101 cgacccaggc gcacatccat ctcgattgac ggccgagtaa aacggcacaa agcgcccgct
     2161 cctctgtacg atgtcgaaca gacgcggcgc cttgccatgc gttgtcattg gcgcgcggtg
     2221 ctgccagcga gcggcgcagg cagcccgctt gtccacgcgc gccaggagcc tctattgacc
     2281 ccttccccat ccggcttgag ttcaaccgaa gccggcctcc ggctgaagcg cgatggcgcg
     2341 aacgccatcg tcgacgtaaa gccgcatccg ctgtggcgcg ccgtcaagaa gctctgggcg
     2401 cccgtgccgt ggatgctcga ggccgccatc gtcctgcagg tgggcctggg cgactatatc
     2461 agcgcgtgca tcgtcctctt tctgctgctc ttcaatgccg gactcgggct cttccaggaa
     2521 ggccaggcgc aggcgaccgt cgatgcgctc aagtcgcgcc tcgccttggt ctcccccgtg
     2581 ctccgcgatg gcgcatgggc caccgtgccc gcggccgagc tggtggcggg cgacatcgtc
     2641 aagctgtcgc tgggctccgt ggtcgccgcg gacgtccgcc tgatcagcgg ctccgtgctg
     2701 ctcgaccagt cgatgctgac cggcgaatcg gccgcgaccg aatcaggcgc ggactcgcag
     2761 gcgtttgccg gcgcgctcgt gcgccgcggc gaagcggtgg ccgaggtcgt cgcgacgggc
     2821 gagcgcacca agttcggccg gaccgcggaa ctggtgcgca cggccaaggt ggaaagctcg
     2881 caacagaaaa ccgtgctgcg ggtcgtgcgc aatctggtgc tgttcaatgg cgcggtaacg
     2941 atcctgcttg gcgcctatgc gttgctggtc tcgatgccgc tcgacgagat cgtgccattg
     3001 ctgctggtgg cgattctttc ctcgattccg gtcgccctgc cctccatgtt cacgctggcg
     3061 gcggcggtgg gcgcccgctc gctggcgagc cggggcgtac tgccgacccg cctctccgcg
     3121 gtcgacgaag ccgcgggcat cgatatcctc tgctccgaca agaccggcac cctgacgcgc
     3181 aacgaactgg cggtgatgtc ggtccgcccg atgccgccgt tcgacgaggc ccacgtcctc
     3241 gccttcgccg cgctcgccag ttccgacggc ggccaggacc ccgtcgacag cgccatccgc
     3301 ggcgcatccg ccggcaagcc gatcgcggac caaccggcgc tcgtggcgtt cgtgcccttc
     3361 gatccggcgc tcaagcgcgc cgaggccacg ctgcgccatg cgaatggcga ggagcggcgc
     3421 gtgctcaagg gcgcgttcgc caccatcgcg gcattgtcca ggccgtgccc gagcgcggcc
     3481 gccatcgtgg ccgagatgga aggatcgggc ttcagggtgc tggccgtcgc cgaaggcagt
     3541 gccaacgacc tcgcgctggc cggcctgatc gcgctcagcg acccgccccg cagcgattcc
     3601 gcggcgctcg tcaccgagct gcgcgacatg ggcgtgcgca ccgtcatggt gaccggagac
     3661 gcgcgcaaga cggccgagac cgtggcggcc tccgtcggca tcagcggaac ggtctgggcc
     3721 gcgacgcccc tgcccggcga tctggacgtg caaccctatt caattttcgc gggcgtgctg
     3781 cccgaggaca agtatcagct ggtcaagtcc ctgcagcagc gcggacatat cgtcgggatg
     3841 tgcggcgacg gcgcgaacga cgcgccggcg ctgcgccagg cgcagatggg cattgccgta
     3901 tccacggcga ccgacgtggc caaatcggcg gcgggcgtgg tgctgaccga accgggcctg
     3961 ggcggcatcg tcgcgctggt caaggaaggc cgcaccactt ttcagcgcat tctcacctac
     4021 acgctgcgtt cgatcatcca caagaccgtg caggtgctgt tcctcgccgc cggcctggtc
     4081 atgacgggcc acgcgatcct cacgcccacg ctcatggtgc tgatgatggt gaccggagac
     4141 ttcctggcca tgtcttcgtc caccgacaac gtccggccct cgccgcggcc gaatatctgg
     4201 cgcatccgca acctgaccct cgccggcatc gcgatgggca tgatcgacct gctgttctgc
     4261 gtggcgtgcc tcgcgacggg aaaattcgcg ctcggcctcg gcaccgatgc gctgcgcacc
     4321 gtcgccgtcg tcacgctggt gttcagcggc caggccgtgt tctacgtcgc gcgggaacgg
     4381 cagcacctgt ggagctcgcc gccggggcgc tggctcgtcg tctcgtcgat agccgatgtg
     4441 gcgatcatcg gcgtcctcgc gatcaacggc attctcatgg cggcattgcc gctggacatg
     4501 atcgcgggct tcctggccgc cgccgtgatc ctggccttca tcctggacac gctcaagcaa
     4561 gtgctgttcc gccgtcttgc gatagtgtga ccgcccggct gcgcggtccg gatcagaaga
     4621 tgcatgcgca gcgcggtatt agacgacctc gatctggtcg tcgacatgat agacgccggg
     4681 tgccgtccac gccgcgttct ccgcgagttt gcgctccggc caactgtgta ctcgacccgt
     4741 caacgtgacc tgattgccgg cgacagtgac cgaaatagcg gccgtctcgt catggaaccg
     4801 gcgcttcagc gcggcctcga tgtccgcctt gacggcggac acggacacct tgggcttgat
     4861 ctcgatctga ttgctgaccc cggtcactcc catcaggtag cgcaccgcct tcgtcgcaga
     4921 ctgacgttcg tagtcccaat cgacttggcc atccagggtg atccagcctt tttcgaccgt
     4981 gacgttgatg cagccgtccg gaatgaccgt gctccagcgc agtgcgttgt cgaccgcgct
     5041 tgcgatttcg gtgtcattgc gcgtgtgcgt atcgatcagc ctgatgtcga cttccaccgc
     5101 gagggttttg acgcccgcca cgcgacgggc ggcacgttgc gccgcgagct tttccgcgta
     5161 actgccgaca tggccggcga gcgtgaccac cccatcgttg acctcgacgc ctatcccggc
     5221 ggctggcagc gaaggctccc atttcagttc ttcgagcacg tcccgctgaa tctgtgtgtc
     5281 ggttttcatg gttcacatcc tgataagggt ttgaagaaac accaggatgc gcccagtcat
     5341 ccgtcaggtc tgtgcgttgg cccacggagt cgcatcatgc gggcggaaat ggcatgccag
     5401 ccccttcatg cctgcccgag caacgccgcg tcgatcgcct gcagcagacg gggatcgtcg
     5461 gcggcgacgt ccggggcaaa acgccccacc acgcggccat cccggccgac caggaatttc
     5521 tcgaagttcc acaggacttg cggcgccgga ttcggcgcca tcccatatcc cttgagccgc
     5581 tcgcggaacg ggccgtcgcc ggtcgcatcg ggctgcgctg cggtcagcgc ttgatagagc
     5641 gaatgctggt cgtcgccgac gaccgatatt ttcgaaaaca acgggaacgt cacgtcatag
     5701 gtcgtgctgc agaactgccg gatctcctcg tcgctgcctg gttcctgtcc cttgaagttg
     5761 ttggcgggaa aacccagcac ctccagcccc tggccgcgct tgtcctcgta gagcgtttcc
     5821 aggccttcgt actgcggcgt cagcccgcat ttggaggcaa cgttcacgat cagcaatacc
     5881 ttgcctcggt aggctgccaa tgtcagcgcc gccccgtcaa tggatttcac cggaatgtca
     5941 tagatcgtct gggtcatggt cgtattcctc tgggaaaata agatggcctg tcggccccgg
     6001 gggcccggac cgggctgccg ggatgcgatc agtcaatcaa aaccgtacac catcagatgg
     6061 aattcggcat gcaatccact ccatgcagcg aaggttcgtt tgggggacat ggttgaatcg
     6121 gtgcatagcc ggagccggcc acaacgcatc cggccaccat tcgggtcttg ctacggcaat
     6181 ggccgcccgc ggtgcagcga ctggatgcac tgggctatcc agcgggccaa gccgtgcctg
     6241 gttacgggaa tttccgcctg gggctgcatc aatgcctggc cttcgcgctc cctgtccgca
     6301 agcctcgccg acaaggcgtg cgctacatcg ggatgcgctt gcaacagccc tgcgaccgcc
     6361 tcgcccggca ggtcgtacac aatggcgttg gtccgggcaa caatggcgag tttgatgggc
     6421 acgcccatga tcggaccgct ttctccgaga tactcacgcg gcccgaatcg cagaatgtcg
     6481 gacgacggat cccccgtgtt catcgtcgcc gccagaatcc cgtagccgac gatcgtaatc
     6541 gcccggggca gctcgccggc cgccagcaac tggtctccct cggtcagttc tcgcctgacg
     6601 agggctgccg cgagctttac ccggtcgtcc ttggaaagca ggccgaagac ccggacgcct
     6661 tcgacgagct ggtactccag cgtttgcgtg ccaatgccac gggccggcac gatcggcgcg
     6721 atcaacgtat gaatcgattc cagatgccga tgcgcggcat cgtaaaagct gttgagcacg
     6781 tcctccgcct gccagcgcgc caaggcgaag aaactgactt cgtaatcggt ggcctcgaac
     6841 ccgatcgact tggtgacgat cacgggtttg ggataggcgg caatgccgac gcaagtgttg
     6901 gcggcaagct gcaaggcctg caccacatcg gatggccggt attgcgaggg cgcctggaac
     6961 cgggcgaccg tcgtatgagg tccgctcggc acgctggcgt tcacgatccg cgacttggcg
     7021 atcgtgctgt tcgggacgac gatcaagtcg cgattcgccc tcgcgagatg cgtcgcgcgc
     7081 caggtaattt caacgaccgt tccttccgtg ccgccgtcca gggaaatcga gtcgcccacc
     7141 cgatacggcg cggtggcgtt gatcaacagc cccgagaaca ggtccgacag cgtgctctgg
     7201 agcgccagcc ccagcacgat ggccagtacg cccgaggtgg ccagaacgcc ccgcagcggg
     7261 atatggaaga cgtcggtaat gatggccagc accgcgctca gatacaaaag cgtgccgatc
     7321 acgtccaaca tgaagcggcg ctcgcgcaga cggccgccaa gcgtatagta agtacgcacc
     7381 actgccgtca cggtggacgc cgccagcatc caccaggcaa cttccagcat cccccagata
     7441 aagcggattt ccggctcggc cgcatggtag agcacctgcg tagggacgat gtcatcatga
     7501 agcatcgcca cggtcagcag cgcgaatgct gcgcaccggc ctagggtgcg cagcccgagc
     7561 cggttgggca ttcccagcag cacgagcagc aggttcaggc cggcggccag gcacgacggg
     7621 atcaattgca atatggggtg gggagacata ttggattccc tgaaaagccg acgcccgggc
     7681 ggcgtacaaa ttggaccacg cggcggcggc aatgcccatg ttacggcggg ggcgggcgcg
     7741 accgaccgtg ggaggtgcca attcaggcct gggtccaggc cttggacatc atcaaggaaa
     7801 agggcttaca gaccgggatc tgcaagccct tgattttctt ggtcggggcg gcgggattcg
     7861 aactcgcgac cctctgctcc caaagcagat gcgctaccag gctgcgctac gccccgacat
     7921 gcttcgcaaa gccccggatt gtactcgaag ccgtcccctg gcatcccgcc cggacaagcc
     7981 tggttcatac ttggcagaaa ccaaacgcgt ccaccccggt cttaacccga taaagcctac
     8041 cggagaaccc ccatgcttac ccccgatgca gcacgcatgc ttgccgacta caaaaagtgg
     8101 gcgaaccagg aaacctacgc catcgtgctg gcgctgcctc ccgaagaaat ctataaggag
     8161 cgtgtcagcc tgttcaagaa cattgcccgc acgctcaacc acatctacgt ggtcgacctg
     8221 atctggcaag cccacgtcga gggacggccg cacggcattg ccgcgctgaa cacggcgacg
     8281 cacgaggaaa tcggcgcgct atgggaagcg cagcaggcca tggacgactg gtatatagcc
     8341 tgggcggcgc agctgaccga agccggcctg gccgaggaca agcgctacga actgatcggc
     8401 ggcaataccg gcagcatgag ctgcgcggag atcctgctgc acgcggtcaa ccacggttcg
     8461 tatcaccgcg gcttcgtctc cgacctgctg acgcagattc ccgcgaaacg cccgacggtg
     8521 gacctgccgg tgtacaagcg cgtccgcgcc gcggcggcgg cggcggcggc ctgacagccg
     8581 gaatggaacg ccagcgcaca gtctcgccgc cgggaatgga ataaaccagg tgcttaccgt
     8641 gttcactccc atgaacccac cttacggagg cattatggtt cgcaaactga ctctttccat
     8701 cctgggcacc gccgccctgg cgatcgccgg acacgcctat gccgcccccg gcatggccag
     8761 caatggcgtg ctggtcggcg acaagggcat gacgctctac accttcgaca aggacgccgc
     8821 caacaccggc aagagcgtct gcaacggcga ctgcgccacc aagtggccgc ccttcgccgc
     8881 gccggccagc gcccaggccg acggcgcctg gaccgtggtc acgcgcgacg acggcagcaa
     8941 gcaatgggcc taccagggca agccggtgta ttactggttc aaggacgcga agcccggcga
     9001 caagaccggc gacggcgtcg tcggggtttg gcacatcatc aagccctgat ccgcgccatg
     9061 ccatcggccg agggcgtaga ccagatcctc gcccacatcc ccgcgctgcg ccgctatgcc
     9121 cgcctcctgg cgggcgacgc ggccagcgcg gacgaccttg tgcaggacac gctggaacgc
     9181 gcctgcacca aatggtcgct ctggaaaccg ggtacggcgc tgcgcgcctg gctgatgacg
     9241 ctgatgcaca acctctacct gaaccgggtg cgcgactggg gccgggacga caaccacgac
     9301 atctgggacg aagcctcggg ccccacccat gaaccgctgg cgcatgcccc ggaactgctg
     9361 gacctggaac gcgccctggc gcggctctcg cccatgctgc gcacggtact gctgctggtg
     9421 ggcgtggaag agtacaccta tgccgaagcc gccgagatcc tgcagatccc catcggcacg
     9481 gtcatgtccc ggctgcaccg cgcgcgcgag gcagtacggc tatcaatgaa cggcgcggcc
     9541 gatgccgccg cgccgacgcc cctgcatctg gtgaaacgat gaccgatacc gatgccttgg
     9601 tcaccgacga cgaactgcac gcgttcgtcg acggccaact cgacgccgcg cggatcgcag
     9661 ccgtgctgca gcacctgcag cagcatcccg aggccgcggt gcgcgcggcc cagtggcatg
     9721 cgcagcgcct cgccctgcgc cgcttgcggc gcgccgccga tccgggcccg acgcccgatg
     9781 cgctcacgca gacggtcctg cgccgccggc ggtcccgctg gaacccctgg acgcaggccg
     9841 ccgccgcgat gctgctggtc gccgcgggcg cggccggcac gcaggcatgg caagtggcga
     9901 ccacccctgc ggcggccacc gtcgccgcca ccgcgccgca attcgtgcag gacgcgacga
     9961 cggcctacgc ggtctatgcg ccggaggtgc gccaccccgt ggaagtcacg gcgcaggaag
    10021 aagcgcacct ggtccaatgg ctcagccgcc ggctgggccg gccattggtc gcgcccgatt
    10081 tgcagggcca gggctttcac ctgctgggcg gccggctatt gccgggcgaa ccggccggca
    10141 cggccgcgcc cgcctcggcg gcgagcgatg ccggcgcttc cggcaacccc gccgcgcgcg
    10201 cgcagttcat gtacgaagac acgcacggcc gccgcgtcac gctctacgtg gcggtctttg
    10261 cacccgatgt cgcgccgcgc gagaccgcat tccgttccgt ctcgtccgcc agcggcgtcg
    10321 ccttctactg ggtggacaac ggctatggct atgcgctgaa cggcgacctg ccggcggccg
    10381 aattgcagca cctggccggc ggggtgtatg accagttgtt cccgcggtag gcgcccgcgg
    10441 caagaagctg cgaggcctgt ggcgcgcggt acttaggcgg ttcctgaagg ccttgcatcc
    10501 agccctgccg gcctgcggcc ctccacggcc ggcagcgaaa aacgcaaggc cgcgccgcgg
    10561 cccggcaggt cgatcagcgc gatcccgcag ccgtgcagcg ccaggatgcg gtgcacgatg
    10621 cgcaagccca gcccgccgcc gcgccgcgcg ccgcccacct cgaaaggctg gcgaaacagg
    10681 cccgcgcgca actcggccgg gatgccgggc ccggtgtcgc tgaccgtgac gtccacgaac
    10741 gcatcccgca ccgccagcgc gacctcgatc tcgccgccgg ccggaacgta gcgcaatgcg
    10801 ttgtccagca ggttggtcaa cacgcgctcg atcatgccca ggtcggcgcg cacgggcggc
    10861 acccgctcgg tgaaacgggc ccgcaacgca atctggcgcg actgggccgg cagctcgaat
    10921 ttctcgaaca cgtcctgcac caggtcgacc agcgagaacg gctcgatctc gggccgcacc
    10981 gcgccgtatt cgaggcgcgc cagctcgaac agcgactgcg ccagccggct caccttgccg
    11041 ctctgatcca gcgcgatgcc gagatagcgg cggcgctcgg acggcgccag cgtagcgtct
    11101 ttcaagacca gcgtctccag atagccgtgt atcgaggtca agggcgtgcg caggtcgtgc
    11161 gagatattgg cgatcagttc gcgccggtcc tggtcctgcc gcgtcagcgc gcgccattgc
    11221 tcgcccaggc ggccggccat ctggcgaaag gccccatcga gcacggcgat ctcgtcgccc
    11281 ccggcgcccg gcggctcggc ggccggcggc agcgcggccg gcgcgccatc gatgtcgaac
    11341 cccttgaccg tatcggtcag gcgccgcagc ggccgggtga tcaacccgaa agccgccagg
    11401 cccgccacca ggcacagcaa ggccaccagc ccgatcgacc agagcccgac gtccagcgcc
    11461 gtggacgtgg cctcgctggc atcgaggcgg tcgtgcgcct cgctcagcag caccacgtag
    11521 acatagccca tctcgcgtcc gtcgacgcgc agcggcgcgg cgctgaacac tttgcgaccg
    11581 cgttcgctgc gcgggtcgtc gcccagcacc ggcaggggcg cgccggccag cagcgcgcgg
    11641 accggcccca ggtcgacatg gtcgcgcatg atccggttgg ccggcgcggc atggccggtg
    11701 atgcggccgt cctggtccag caaatagacc tcgacgctgg ggttgaccac catgagctgg
    11761 cgaaacagct ggcgcacggc gtccggcttc agtccgctgc cgtcggccag ttggccgtcg
    11821 cgcgcgatgt gctcggccag gtcgcgcgac agggcctgga cgacctccag cgcatgcatg
    11881 cgattggagc gtatctgcag ccatgccgaa gtgccgctgc acacggccaa cagcagcgca
    11941 aacaccagcg ccaaacgctg ggtgagggtc aaccggcgca gcagtttcac gagggcttgc
    12001 cctcgggcac ggcaaacttg tagccgcgcc cccataccgt caggatgtgc tcgggctgcg
    12061 ccggatccac ctcgatcttg gcgcgcagcc ggttgatgtg cgtattgacc gtgtgctcgt
    12121 agccatcgtg gctatagccc cagacggcgt tgagcaagtc catgcgcgaa aacaccttgc
    12181 cgggctggcg cgcgaagaaa tacagcaggt cgaattcgcg cggggtgaga tcgagccggc
    12241 ggcccgcaag cagggcctcg cgcgcgatcg ggtcgatgaa caggccgccc accgacaggc
    12301 tgccggcctc gatgcgggca ttctgcgaca aggcatcgac gcggcgcagc agcgccttga
    12361 cgcgcgcgat cagctccagc accgagaacg gcttggccag gtagtcgtcg gcgccgagct
    12421 ccaggccgag gatgcggtgc acctcgctgg cgcgcgcgct gaggatgatg atggggatat
    12481 agcgcggcat ggcgcgggcg cggcggcaga tctcaaggcc gtcgatgccg ggcagcatca
    12541 ggtcgaggat gagcgcgtcc caaccgccct gctcgagctg ccgcaggcca tcctcgccgt
    12601 gggcgcagtg ggtcacgtca tagccctcgt cgcgcaggtt gagcgcgagc aggtcggcga
    12661 tttgcatgtc gtcctcgacc acgaggacgc gtctgggctg atccatcggc gggaagttca
    12721 ggtgccgcgc tggtggcgcg atcgcgacat tgtgggcttt tccggcaggc caagttatca
    12781 cgatttgttt aagtctgcgt gaggactccg ggaccgccgc gccgctacgc tacgcccatc
    12841 ctgtcatgac cctcgggaga tttccatgct gacacgacga caattcttct acctgggcgg
    12901 cggcgccctg ctggcggcgg gcgtgctgcc ccggatcctg gccggcacgg cctccgcggc
    12961 cccggaagcc gcggacggcc cgttcgaggt cacgcatacc gaggccgaat ggcacgcgct
    13021 gctgacgccc gcgcaatacg ccgtcctgcg cgactcgggc acggaacggc cgtacagcag
    13081 cccgctggac gacgagcacc gggccggcac cttcgcctgc gccggctgcg gcctgccgct
    13141 gttctcgtcc gcaaccaagt tcgacagccg caccggctgg ccgagcttct gggcggcgat
//
LOCUS       DASWPO010000019         8374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_717791, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000019 DASWPO010000000
VERSION     DASWPO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717791"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGC69_00780"
     CDS             complement(<1..230)
                     /locus_tag="VGC69_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0293846.1"
                     /translation="MLETRDLTIRFGGHTAVDQVSCRYEPGTLTSIVGPNGAGKTTYF
                     NLISGQLRASAGSVHLDGRNITALGAAARMHAG"
     gene            complement(251..1423)
                     /locus_tag="VGC69_00785"
     CDS             complement(251..1423)
                     /locus_tag="VGC69_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0293847.1"
                     /translation="MKKLLLAALAALAMHAPAWSADFVIAHVYDKTGPLEAYAKQTQT
                     GFMMGLEYATHGTMEVAGRKIRVIEKDDQGKPDVGRAQLASAYADDQADIAVGPTSSG
                     VALAMLPIAEEYEKILLVEPAVADSITGDKWNRYIFRTGRNSSQDAIANAVAFDQDGT
                     SIAMLAQDYAFGRDGVKAFKSALKHAKIVHEEYLPVDTTDFTAGAQRLFDALKDKPGR
                     KIIFILWAGAGNPFKIADLDPKRYGIEIATGGNILPATAPLKALPGMEGATYYYYGMP
                     KNPINDWFVAEHQKRFGQPPDFFTAGGMSAALAIVAALQKTQGKSDTDTLIKAMEGMS
                     FDTPKGRMTFRPQDHQAMQSMYHFRISNDASMPWAVQDLVKEITPDQMDVPILNKR"
     gene            complement(1447..2337)
                     /locus_tag="VGC69_00790"
     CDS             complement(1447..2337)
                     /locus_tag="VGC69_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0293848.1"
                     /translation="MHDTPLSESTGNARAWPVSHYASCLGRELHFLEWGQPAAPAVFL
                     WHGLARTSRDFDDLAQVLAARYRVICPDTIGRGLSQWSANPVEDYRLDAHARLAAALA
                     DALHLSQLRWVGTSMGGATGLVAAATTLRGRISHLVLNDIGPELPAPAAQRISAYVGN
                     PPVFDTVTELEAWLRQAYRPYGWQSDAQWRRMAETSVRRLPDGRVTLHYDPAIVGQFQ
                     HHPRDYDRWEEYDSLRLPVLLLRGAESDLLLEDVADAMTRRGPRAQRIDFEGCGHAPA
                     LNVPPQIDAVAGFLAQPGAL"
     gene            2531..3499
                     /locus_tag="VGC69_00795"
     CDS             2531..3499
                     /locus_tag="VGC69_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0293849.1"
                     /translation="MGCGHTVPDGMPDGGRQGYEQWREALNDAFGPFEVHCGPPADFA
                     GRVRYVRRAGLQFNDLHYQGQQIERTSHNVSLLSEEFYTFGLPLSGPLEVSRSGRQFQ
                     VEPGCVYLMNQSAPYRAAAQGTQGYRSLSISFPREALERRHARIEPFYKLAIGDGSPR
                     GALLAQYLEQVFKGLHDWSDAEVAELGERLIDLIVLFLVEPGRAQASEADSSVTLAHR
                     ERIEAYIKRHLGDPALRPEAIAQACGISPSYLHRIFHGTGVEAYLYEQRLLYCRDLLA
                     SARHAHRSIAELAYRAGFVHPSHFSRLFKRHFGVSARAFRADRLPK"
     gene            complement(3573..5000)
                     /locus_tag="VGC69_00800"
     CDS             complement(3573..5000)
                     /locus_tag="VGC69_00800"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0293850.1"
                     /translation="MYESLALYINGEFIGGDGRKTEDVVNPATGEILGQLPHATQADL
                     DHALESSQRAFESWKRTSPLERSAILRKVAQLSRERAQEIGRNITLDQGKPVAEAALE
                     ITSCSEHLDWAAEECRRIYGRVIPSRNPDVRQFVVREPVGVCAAFTPWNFPYNQAIRK
                     IAAALGSGCTMVLKGPEDSPSAIMAIARMFHEAGLPKGCLNIVWGKPAEVSDYLIRSP
                     IVRKVSFTGSVPVGKQLAALAGQYMKRGTWELGGHSPVLVFDDADIDRAATMLAKFKV
                     RNAGQVCVSPTRFYVQKGAYDQFVAKFTDVLKGIKVGNGLEAGTEMGPLAHARRVPAI
                     EKFVDDSIKRGGKILTGGEAPSGKGFFFSPTVITGLPDDSMLMTEEPFGPIAPVVPFT
                     DADEVLKRANNLPFGLSSYVFTNSLQTATKVSNGLEAGMVNINHFGSALAETPFGGVK
                     DSGIGSEGGTEMFDGYVVTKFITHI"
     gene            5522..5890
                     /gene="rplN"
                     /locus_tag="VGC69_00805"
     CDS             5522..5890
                     /gene="rplN"
                     /locus_tag="VGC69_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY0293851.1"
                     /translation="MIQMQTTLDVADNTGARRVMCIKVLGGSKRRYAGIGDVIKVSVK
                     DAAPRGRVKKGEIYNAVVVRTAKGVRRKDGSLIRFGGNAAVLLNAKLEPIGTRIFGPV
                     TRELRTEKFMKIVSLAPEVL"
     gene            5902..6222
                     /gene="rplX"
                     /locus_tag="VGC69_00810"
     CDS             5902..6222
                     /gene="rplX"
                     /locus_tag="VGC69_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY0293852.1"
                     /translation="MNKIRKGDEVIVLTGRDKTRRGTVLSRIDADHVLVEGVNVVKKH
                     AKPNPMAGIPGGIVEKTLPIHVSNIALFNPATGKGDRVGFTEVDGKKVRVFRSSGAVV
                     GAKA"
     gene            6235..6774
                     /gene="rplE"
                     /locus_tag="VGC69_00815"
     CDS             6235..6774
                     /gene="rplE"
                     /locus_tag="VGC69_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY0293853.1"
                     /translation="MTRLQDFYKNQVVGDLQAKFGYKSLMEVPRITKITLNMGVSEAV
                     ADKKVIEHAVSDLTKIAGQKPVITKTKKAIAGFKIRENYPIGCMVTLRGQRMYEFLDR
                     LVAVALPRVRDFRGISGRAFDGRGNYNIGVKEQIIFPEIEYDKIDALRGLNISITTTA
                     KTDEEAKALLTAFSFPFRN"
     gene            6787..7092
                     /gene="rpsN"
                     /locus_tag="VGC69_00820"
     CDS             6787..7092
                     /gene="rpsN"
                     /locus_tag="VGC69_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY0293854.1"
                     /translation="MAKLSLINRDIKRAKLAEKFSAKRAELKATIDDQSKTDEERYQA
                     RLKLQQLPRNANPTRQRNRCVVTGRPRGVFSKFGLTRHKLREMAMKGEIPGMTKASW"
     gene            7105..7500
                     /gene="rpsH"
                     /locus_tag="VGC69_00825"
     CDS             7105..7500
                     /gene="rpsH"
                     /locus_tag="VGC69_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY0293855.1"
                     /translation="MSMSDPIADMLTRIRNAQQVDKVTVAMPSSKLKVAIAAVLKDEG
                     YIDSFQIKGDQAKPELEITLKYYAGRPVIERIERVSKPGLRIYKGRSAIPTVMNGLGV
                     AIVSTSRGVMTDRKARANGVGGEVLCYVA"
     gene            7513..8046
                     /gene="rplF"
                     /locus_tag="VGC69_00830"
     CDS             7513..8046
                     /gene="rplF"
                     /locus_tag="VGC69_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY0293856.1"
                     /translation="MSRIAKYPVELPKGVEANISAGQITVKGPLGTLSQTLTGEVAIR
                     QEDSKLTFAVLDESRHANAMSGTLRALVNNMVTGVSKGFERKLTLVGVGYRASVQGDA
                     VKLQLGFSHDVVHALPTGVKAECPTQTEIIIKGSDKQVVGQVAAEIRAYREPEPYKGK
                     GVRYADERVVIKETKKK"
     gene            8068..>8374
                     /locus_tag="VGC69_00835"
     CDS             8068..>8374
                     /locus_tag="VGC69_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY0293857.1"
                     /translation="MDKKVTRLRRAVPTRRKIAELRVHRLSIFRSNQHIYANIISPEG
                     DRVLVSASTLEAEVRAELAGKTGLGGNTAAAALVGKRVAEKAKAAGIELVAFDRSGFR
                     "
BASE COUNT         1476 a         2613 c         2854 g         1431 t
ORIGIN      
        1 ccggcatgca tgcgggccgc cgcgcccagg gcggtaatgt tgcggccgtc caggtgcacg
       61 gagcccgcgc tggcgcgcaa ctgccccgag atgaggttga aataggttgt cttgccggcg
      121 ccgttggggc cgacgatgga ggtcagcgtg ccgggctcgt accggcagct gacctggtcg
      181 acggccgtgt gcccgccgaa gcggatggtg aggtcgcggg tttccagcat ggacgatgaa
      241 agcgtgtctg tcagcgcttg ttgaggatgg ggacgtccat ctggtcgggc gtgatttcct
      301 tgaccaggtc ctgcacggcc cagggcatcg acgcatcgtt gctgatgcgg aagtggtaca
      361 tggactgcat ggcctggtgg tcctgcgggc ggaaggtcat gcggcccttg ggcgtgtcga
      421 agctcatgcc ttccatggcc ttgatcagcg tgtcggtgtc ggacttgccc tgggttttct
      481 gcagggccgc gacgatggcc agcgccgccg acatgccgcc ggcggtgaag aagtccggcg
      541 gctggccgaa gcgcttctgg tgctcggcga cgaaccagtc gttgatgggg ttcttgggca
      601 tgccgtagta gtagtacgtg gcgccttcca tgccgggcag ggccttgagc ggcgccgtgg
      661 ccggcaggat gttgccgccc gtggcgatct cgatgccgta gcgcttcggg tcgaggtcgg
      721 cgatcttgaa gggattgccc gcgccggccc acaggatgaa gatgatcttg cggcccggct
      781 tgtccttcaa ggcgtcgaag aggcgctgcg cgccggcggt gaagtcggtg gtgtcgaccg
      841 gcaggtactc ttcatggacg atcttggcgt gcttgagcgc gctcttgaac gccttgaccc
      901 cgtcgcggcc gaaggcgtag tcctgcgcca gcatggcgat ggacgtgccg tcctggtcga
      961 aggccacggc attggcgatg gcgtcctgcg aggagttgcg gccggtgcgg aagatgtagc
     1021 ggttccactt gtcgccggtg atcgaatcgg cgacggccgg ctccaccagc aggatcttct
     1081 cgtattcctc ggcgatgggc agcatggcca gtgcgacgcc cgacgaggtc gggccgacgg
     1141 cgatgtcggc ctggtcgtcg gcgtaggccg aggcgagctg ggcgcggccc acgtcgggct
     1201 tgccctggtc gtctttttca atgacgcgga tcttccggcc ggcgacctcc atggtgccgt
     1261 gggtcgcgta ttccagcccc atcatgaagc cggtctgggt ttgcttggca taggcttcga
     1321 gcgggcccgt cttgtcgtag acatgggcga tgacgaagtc ggccgaccag gccggggcgt
     1381 gcatggccag ggcggccagg gcggccagca gcagtttttt catgggtctc tccttcctcg
     1441 cggttcttag agcgcgccgg gttgggccag gaagccggcc acggcgtcga tttggggggg
     1501 gacgttcaag gccggcgcat ggccgcagcc ttcgaagtcg atgcgctgcg cgcggggacc
     1561 gcgccgcgtc atggcgtcgg ccacgtcctc gagcaggagg tcggactcgg cgccgcgcag
     1621 cagcagcacg ggcaggcgca gggagtcgta ttcctcccag cggtcgtagt cgcgcgggtg
     1681 atgctggaac tggccgacga tggccgggtc gtaatgcagc gtgacgcggc cgtcgggcag
     1741 gcggcgcacc gaggtttccg ccatgcggcg ccattgcgcg tcgctctgcc agccgtaggg
     1801 ccggtaggcc tggcgcagcc aggcctccag ctcggtcacg gtatcgaaga cgggcggatt
     1861 gccgacatag gccgagatgc gctgcgcggc cggggcgggc agctcgggac cgatgtcgtt
     1921 gagcaccagg tgcgagatcc ggccgcgcag cgtcgtggcg gcggcgacca gcccggtggc
     1981 gccgcccatg gacgtgccga cccagcgcaa ttgggacagg tgcagggcat cggccagcgc
     2041 ggcggccagg cgggcgtggg cgtccagccg gtagtcctcg acggggttgg cgctccactg
     2101 cgacaggccg cggccgatgg tgtcggggca aatgacgcgg taccgggcgg ccaggacctg
     2161 ggccaggtcg tcgaagtcgc ggctggtgcg ggccagcccg tgccacagga acacggcggg
     2221 cgccgcaggc tggccccatt cgagaaagtg cagttcccgg cccaggcagc tggcatagtg
     2281 cgaaaccggc caggcgcggg cattgccggt cgattcggac aagggggtgt cgtgcatggt
     2341 ctcctcccga aggcgcaggc gcgggcctga cgtatcgtat gtatcgttct acgggtatcc
     2401 tccgactata gacgtgcgga aaatgcgcgc gcaatacatc gctcgcctgg tgcaggctga
     2461 gtcaatagaa tgtggagcct gggtaaagtc cagacggcgc agttccccgg gagacatcgc
     2521 catgacagca atgggttgcg ggcataccgt cccagacggc atgcccgacg gcgggcggca
     2581 aggctacgaa caatggcgcg aggccttgaa cgacgccttc gggccgttcg aggtgcattg
     2641 cggtccgccg gccgattttg ccggccgcgt gcgctatgtg cgccgcgccg ggctgcagtt
     2701 caacgacctg cactaccagg gccagcagat cgagcgcacc tcgcacaacg tatcgctctt
     2761 gtcagaagag ttctacacct tcggcctgcc cctgtccgga ccgctggagg tctcccgctc
     2821 ggggcggcag ttccaggtgg agcccgggtg cgtgtacctg atgaaccaga gcgcccccta
     2881 tcgcgcggcc gcccagggca cgcagggcta ccgcagcctg agcatctcgt ttccccgcga
     2941 ggcgctggaa cggcgccatg cgcgcatcga gcctttctac aagctggcca tcggcgacgg
     3001 ttcgccgcgc ggagcgttgc tggcgcaata cctggagcag gtgttcaagg gcctgcacga
     3061 ctggtccgat gccgaggtgg ccgagctcgg cgagcgcctg atcgatctga tcgtgttgtt
     3121 cctggtcgaa ccgggccggg cccaggcttc cgaggccgac agcagcgtca cgctggccca
     3181 tcgcgaacgc atcgaagcct atatcaagcg ccacctgggc gacccggcgc tgcggcccga
     3241 ggccattgcc caggcctgcg gcatttcacc gagctatctg caccgcatat tccacggcac
     3301 gggcgtggag gcctatctct acgaacagcg cctgctgtat tgccgcgacc tgctggccag
     3361 cgcgcggcat gcgcatcgca gcatcgcgga gctggcctat cgggcggggt tcgtccaccc
     3421 ctcgcatttc agccgcctgt tcaagcgcca cttcggcgtg tcggcccggg catttcgcgc
     3481 ggaccgcctg cccaaatgat cggcggcggg ggggcgtggt tcgcggacga atcgtccatg
     3541 aatcacaggc cttccatcac cgccttgccc ggtcagatgt gcgtgatgaa cttggtcacc
     3601 acgtagccat cgaacatctc ggtgccgcct tcgctgccga tgccgctgtc cttcacgccg
     3661 ccgaaaggcg tttcggccag cgcgctgccg aagtggttga tgttcaccat gccggcctcc
     3721 aggccgttgg acaccttggt cgccgtctgc agcgagttgg tgaagacgta cgacgacagg
     3781 ccgaagggca ggttgttggc gcgcttgagc acctcgtcgg cgtcggtgaa gggcaccacc
     3841 ggcgcgatcg ggccgaaggg ctcttcggtc atcagcatcg agtcgtccgg caggccggtg
     3901 atgacggtcg ggctgaagaa gaaacccttg ccgctgggcg cctcgccgcc ggtcaggatc
     3961 ttgccgccgc gcttgatcga gtcgtcgacg aacttctcga tggcggggac gcggcgcgcg
     4021 tgggccagcg ggcccatctc ggtgccggct tccaggccat tgcccacctt gatgcccttg
     4081 agcacgtcgg tgaacttggc gacgaactgg tcgtaggcgc ccttctgcac gtagaagcgc
     4141 gtcggcgaca cgcacacctg gccggcgttg cggaccttga acttggccag catggtcgcg
     4201 gcgcggtcga tgtcggcatc gtcgaacacc agcacgggcg aatggccgcc cagctcccag
     4261 gtgccgcgct tcatgtactg gccggccagc gccgccagct gcttgccgac cggcaccgag
     4321 ccggtgaagc tgaccttgcg cacgatgggc gagcggatca ggtagtcgga cacttcggcc
     4381 ggcttgcccc agacgatgtt caggcagccc ttgggcaggc cggcctcatg gaacatgcgg
     4441 gcgatcgcca tgatggcgct gggcgaatct tccgggccct tgagcaccat ggtgcagccc
     4501 gagcccaggg cggcggcgat cttgcggatg gcctggttat aagggaagtt ccagggcgtg
     4561 aaggcggcgc acacgcccac cggctcgcgc acgacgaact ggcgcacgtc cgggttgcgc
     4621 gacgggatca cgcggccgta gatgcggcgg cactcttcgg cggcccagtc caggtgctcg
     4681 gagcaggagg tgatctcgag ggcggcttcg gccacgggct tgccctggtc gagcgtgatg
     4741 ttgcggccga tttcctgggc gcgctcgcgc gagagctggg cgaccttgcg caggatggcg
     4801 gaacgctcca gcggcgaggt gcgcttccag gattcgaagg cgcgctgcga ggattccagg
     4861 gcgtggtcca ggtcggcctg ggtggcgtgg ggcagttggc ccaggatttc cccggtggcg
     4921 gggttgacga cgtcttccgt cttgcggccg tcgccgccga tgaattcgcc gttgatatag
     4981 agggctaggg attcgtacat gatgcggtct ggctccggga aatgggcgag cgcgggacgg
     5041 gaaagccgcg ttcgaagcta agcattgcca gtctaaacca gcctgggccc gccagcccat
     5101 ggcccgctgc cggcatggcg ctcgtccggc aggcccctgg atgcggcgaa cgcggccatg
     5161 atgcgacccc tttaactgtc cgtggtgaat cgggaacagg gggcatgact tgcggaaagc
     5221 gcgaaaaaaa cgcttgcgca aacctgtaga ggcgggctag aatgcttagc tctttccccg
     5281 agaggtatcg gggagggtgt gcgggatggt tgcggcggtg tttgaacttg tatggtcgaa
     5341 tgcgccagta gcctgaaaac acaggaattt ccgcaagatt tcaacccagg ttgctggggc
     5401 ggcacaagcc gcgccagggg ccttacggga ccaaaactga tgaagggttt tacccgaatc
     5461 cactcctcct agaggggcgt tgccggggga aggccttcat taagttggaa caggaaaaat
     5521 catgatccaa atgcagacca cgctggacgt ggccgacaac actggtgcac gccgcgtgat
     5581 gtgcatcaag gtgctcggcg gctccaagcg ccgttatgcc ggcatcggcg atgtcatcaa
     5641 agtcagcgtc aaggacgccg ccccccgcgg ccgcgtcaag aaaggcgaaa tctacaacgc
     5701 cgtggtggtt cgcaccgcca agggcgtgcg ccgcaaggac ggctcgctga tccgtttcgg
     5761 cggcaacgcc gccgtgctgc tcaatgccaa gctggagccc atcggcaccc gcatcttcgg
     5821 acccgttacg cgcgaactgc gtaccgagaa gttcatgaag atcgtgtcgc tggcccccga
     5881 agtgctgtaa ggagccctac gatgaacaag atccgtaaag gcgacgaagt catcgtcctg
     5941 accggccgcg acaaaacccg ccgcggtacc gtgctgtcgc gtatcgacgc cgaccacgtg
     6001 ctggtcgagg gcgtcaacgt cgtcaagaag cacgccaagc ccaaccccat ggccggcatc
     6061 cccggcggca tcgtcgaaaa gacgctgccc atccacgtct cgaacatcgc gctgttcaac
     6121 cccgccaccg gcaagggcga ccgcgtcggt ttcacggaag tggacggcaa gaaggtgcgc
     6181 gtgttccgtt ccagtggtgc cgttgtcggc gccaaggcgt aaggagcgat agacatgact
     6241 cgtttgcaag atttctacaa gaaccaggtc gtcggcgacc tgcaggccaa gttcggctac
     6301 aagagtctca tggaggtgcc ccgcatcacc aagatcacgc tgaacatggg cgtttcggaa
     6361 gccgtggccg acaagaaggt catcgagcac gccgtgtccg acctgaccaa gatcgccggc
     6421 cagaagcccg tgatcaccaa gaccaagaag gctatcgccg gtttcaagat ccgcgaaaac
     6481 tacccgatcg gttgcatggt gaccctgcgt ggccagcgca tgtacgaatt tctcgatcgc
     6541 ctggttgcgg ttgcgctgcc tcgcgtgcgt gacttccgcg gtatctcggg ccgtgccttc
     6601 gacggccgcg gcaactacaa catcggggtg aaagagcaga tcattttccc cgaaatcgag
     6661 tacgacaaga tcgacgcgct gcgtgggctg aacatcagca tcaccaccac cgccaagacc
     6721 gacgaagaag ccaaggcgct cctgaccgcc ttcagcttcc cgttccgtaa ctaaggggct
     6781 gaggacgtgg ctaaactttc cctcatcaat cgcgacatca agcgcgccaa gctggccgag
     6841 aagttctcgg ccaagcgtgc cgagctgaaa gcgaccatcg acgatcaatc gaagaccgac
     6901 gaagagcgtt accaggctcg cctgaagctg cagcagcttc cgcgcaatgc caaccccacg
     6961 cgccagcgca atcgctgcgt ggtcaccggc cgtcctcgcg gcgtgttcag caagtttggc
     7021 cttacccgcc acaaactgcg tgaaatggcc atgaagggcg aaatccccgg catgaccaag
     7081 gccagctggt aaggagaata gaacatgagc atgagcgatc ctatcgccga tatgctgacc
     7141 cgcattcgca atgcgcagca agtggacaag gtcacggtgg ccatgccctc gtcgaagctg
     7201 aaggtggcca ttgccgccgt gctgaaagac gaaggctaca tcgacagctt ccagatcaag
     7261 ggcgaccagg ccaagcctga gctcgagatc accctgaagt actacgccgg ccgtccggtc
     7321 atcgagcgca tcgaacgcgt ctcgaagccc ggcctgcgca tctacaaggg ccgtagcgcc
     7381 atccccaccg tcatgaacgg cctgggcgtg gctatcgttt cgacctcgcg cggcgtgatg
     7441 accgaccgca aggctcgcgc caacggcgtg ggcggcgaag tcctgtgcta cgtggcctaa
     7501 ggagaaattc tcatgtcacg tatcgctaaa tatccggtcg agctgcccaa gggcgtcgag
     7561 gccaacatca gcgccggcca gatcacggtc aagggcccgc tgggcacgct gtcgcaaacg
     7621 ctgactggcg aagtcgccat tcgccaggaa gacagcaagc tgacgttcgc cgtcctggac
     7681 gagagccgtc acgccaacgc catgtcgggc accctgcgcg ccctggtcaa caacatggtc
     7741 accggcgtga gcaagggctt cgagcgcaag ctgacgctgg ttggcgtggg ttaccgcgcc
     7801 tccgtgcaag gcgacgccgt gaagctgcag ctgggcttct cgcatgacgt cgtgcatgcg
     7861 ttgcccactg gcgtgaaggc cgagtgcccc acccagacgg aaatcatcat caagggctcc
     7921 gacaagcagg tcgtcggcca ggtggctgct gagatccgcg cctaccgcga acccgaaccc
     7981 tacaagggca agggcgtgcg ctatgcggac gagcgcgtgg tcatcaaaga gaccaagaag
     8041 aaataacggc gcacgcaagg acgaatcatg gacaagaaag ttacccgttt gcgtcgtgcg
     8101 gttccgaccc gtcgcaagat cgccgagctg cgcgtacacc gcctctcgat cttccgctcg
     8161 aaccagcaca tctacgccaa cattatttcg ccggaaggcg accgtgtgct ggtcagcgcc
     8221 tcgacgctgg aagccgaagt gcgcgccgaa ctggccggca agaccggcct gggcggcaac
     8281 actgccgctg ccgcattggt gggcaagcgc gttgccgaga aggccaaggc cgccggcatc
     8341 gagctggttg ccttcgaccg ctcgggcttc cgct
//
LOCUS       DASWPO010000020        12474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2681689, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000020 DASWPO010000000
VERSION     DASWPO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12474
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2681689"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..4330)
                     /gene="rpoC"
                     /locus_tag="VGC69_00840"
     CDS             complement(77..4330)
                     /gene="rpoC"
                     /locus_tag="VGC69_00840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY0293858.1"
                     /translation="MKALLDLFKQVSQDEQFDAIKIGIASPEKIRSWSYGEVRKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTVAKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAWCVIEPGMTPLKRGQIM
                     SDDDFLAKTEEYGDDFRALMGAEAVRELLRTIDIDREVETLRAELKATSSEAKIKKIS
                     KRLKVLEGFQKSGIKPEWMVMEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIILRNEKRMLQEAVDSLLDNGRRGKAMTGANKRQLKSLADMIKGK
                     SGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPFIFNRLEMMGLAT
                     TIKAAKKLVESQEPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPTLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQLEARTLMLASNNVLFPANGEPSIVPSQDIVL
                     GLYYTTRERINGKGEGIFFTDVAEVQRAYDNNEVTLQTRITVRLKEHERDANGEWQPV
                     IRRHETTVGRALLSEILPKGLPFTVLNKALKKKEISRLINQSFRRCGLRDTVIFADKL
                     MQSGFRLATRGGISIAMEDMLIPKAKEGILAEASREVKEIDKQYSSGLVTSQERYNNV
                     VDIWGKAGDKVGKAMMEQLATEPVVNRQGENVRQESFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVTQDLVITEDDCGTSQGYFMKALVEGGEVIEPLRDRILGRVAATDIVNPDTQET
                     AIAAGTLLDEDLVDAIDKLGVDEVKVRTPLTCETRHGLCAHCYGRDLGRGYKVNTGEA
                     VGVIAAQSIGEPGTQLTMRTFHIGGAASRSALASAVETKSAGTVGFTGTMRYVTNAKG
                     ERVAISRSGELAIFDDNGRERERHKIPYGATVLVGDGEVVKAGARLASWDPLTRPIVS
                     EYGGAVRFENIEEGATVAKQVDEVTGLSTLVVITPKTRGGKVAMRPQIKLVNDAGEDV
                     KIAGTDHTVNISFPVGALITVRDGQQVAVGEVLARIPQESQKTRDITGGLPRVAELFE
                     ARSPKDAGMLAEVTGTVSFGKDTKGKQRLVITDMEGASHEFLIPKEKQVLVHDGQVVN
                     KGEMIVDGPADPHDILRLQGIEKLATYIVDEVQDVYRLQGVKINDKHIEVIVRQMLRR
                     VNIVDAGDTSFITGEQVERSELLNENDRVVAEEKRPAIFENVLLGITKASLSTDSFIS
                     AASFQETTRVLTEAAIMGKRDDLRGLKENVIVGRLIPAGTGLAYHLARKEKDAWEAEE
                     RTALLESEKAARAADTAAQTVAIFGNPDDDHGSENDSAE"
     gene            complement(4330..8442)
                     /gene="rpoB"
                     /locus_tag="VGC69_00845"
     CDS             complement(4330..8442)
                     /gene="rpoB"
                     /locus_tag="VGC69_00845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY0293859.1"
                     /translation="MPYSYTEKKRIRKSFAKREDVQNVPFLLATQLQSYLTFLQSDVP
                     SAGRASEGLQAAFSSIFPIVSHNGMARLEFVSYVLGEPVFDVKECQQRGLTYASPLRA
                     KVRLVLMDREVSKPTIKEVKEQEVYMGEIPLMTSTGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDPKDVLFFRVDRRRKMPVTILLKAIG
                     MTPETILGGFFDFDNFELQNEGAMMEVVSERWKGEIARFDISDRSGKVLVEKDKRINA
                     KHLRDMATAGIERISVPEDFLFGRVLAKNIVDAETGEIVAHANDEITESVLSAMREAK
                     VAGFQTLYTNDLDRGPYISQTLRTDETVDQMAARVAIYRMMRPGEPPTEEAVEALFQR
                     LFYSEETYDLSRVGRMKVNSRLGRGDTNVGPMTLTNDDILETIKVLVELRNGRGQIDD
                     IDHLGNRRVRCVGELAENQFRAGLVRVERAVKERLGQAETENLMPHDLINSKPISAAI
                     KEFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCP
                     IETPEGPNIGLINSMALYARLNEYGFLETPYRKIIDGKVSDQIDYLSAIEESHYVIAQ
                     ANAELDEEGRFVDDLVACREAGETMMTAPSNVHYMDVAPSQIVSVAASLIPFLEHDDA
                     NRALMGANMQRQAVPCPRPQKPLVGTGIERTVAVDSGTTVQALRGGVVDHVDAERVVI
                     RVNDDENVAGEVGVDIYNLIKYTRSNQNTNINQRPIVKRGDRVAKGDVLADGASTDLG
                     ELALGQNMLIAFMPWNGYNFEDSILISEKVVADDRYTSVHIEELTVVARDTKLGPEEI
                     TRDISNLAETQLNRLDDSGIVYIGAEVSADDVLVGKVTPKGETQLTPEEKLLRAIFGE
                     KASDVKDTSLRVPSGMVGTVIDVQVFTREGIVRDKRAQSIIDDELRRYRQDLNDQLRI
                     VENDQFDRLEKMLNGKTVNGGPKKLAKGAIVTKAYLADMDRWQWFDIRLADEQHAVVL
                     EQAKESLEQKRHQFDLAFEEKRKKLTQGDELPPGVLKMIKVYLAVKRRLQPGDKMAGR
                     HGNKGVVSRITPVEDMPHMADGTTADIVLNPLGVPSRMNVGQVLEVHLGWAAKGVGQR
                     IADLLRDERTAQVKNVRAYLEKVYNSTGTGARIDELTDDEVIEMAHNLRDGVPLATPV
                     FDGATEEEIGAMLKLAYPDEIAERMQLTPSRAQAWLFDGRTGEKFERPVTVGYMHYLK
                     LHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYTLQEMLT
                     VKSDDIAGRTKVYENIVKGDHVIDAGMPESFNVLVKEIRSLALDMDLERN"
     gene            complement(8633..9013)
                     /gene="rplL"
                     /locus_tag="VGC69_00850"
     CDS             complement(8633..9013)
                     /gene="rplL"
                     /locus_tag="VGC69_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY0293860.1"
                     /translation="MALNKAEILDAIAGLTVLELSELIKDLEEKFGVSAAAAAVAVAA
                     PAAGGAAAVAEEQTEFTVVLTETGANKVSVIKAVRELTGLGLKEAKDLVDGAPKPVKE
                     GVAKADAEAAKKKLEEAGAKVEVK"
     gene            complement(9095..9619)
                     /gene="rplJ"
                     /locus_tag="VGC69_00855"
     CDS             complement(9095..9619)
                     /gene="rplJ"
                     /locus_tag="VGC69_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY0293861.1"
                     /translation="MSLNRQEKAVVIEEVAAQVAKAQSIIVAEYRGLDVASVTVLRKT
                     ARESGVYLRVLKNSLARRAVAGTAFEPLTSQLTGPLIYGISTDPVAAAKVLAGFAKSN
                     DKLVLKGGSLPNNVLNTEGVKALASLPSREELLSKLLGTMQAPIAQFVRTLNEVPTKF
                     ARGLAAVRDQKAAA"
     gene            complement(9889..10584)
                     /gene="rplA"
                     /locus_tag="VGC69_00860"
     CDS             complement(9889..10584)
                     /gene="rplA"
                     /locus_tag="VGC69_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0293862.1"
                     /translation="MAKLSKRAVAIAAKIDRNKFYPVAEALTLVKETATAKFDESIDV
                     AVQLGIDPKKSDQLVRGSVVMPAGTGKSVRVAVFAQGDKAEAARAAGADIVGMEDLAE
                     SIKGGTFNFDVVIASPDTMRIVGTLGQILGPRGLMPNPKVGTVTPDVATAVKNAKAGQ
                     VQYRTDKAGIIHATIGRASFGVEQLQTNLAALVDALQKARPAAAKGIYLRKLAVSSTM
                     GGGARVEIASLSA"
     gene            complement(10587..11018)
                     /gene="rplK"
                     /locus_tag="VGC69_00865"
     CDS             complement(10587..11018)
                     /gene="rplK"
                     /locus_tag="VGC69_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY0293863.1"
                     /translation="MAKKIVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     KTQGLEPGLPIPVVITAFADKSFTFIMKTPPATVLIKKAAGVQKGSPKPHTDKVGTLT
                     RAQAEEIAKTKQPDLTAADLDAAVRTIAGSARSMGITVEGI"
     gene            complement(11125..11658)
                     /gene="nusG"
                     /locus_tag="VGC69_00870"
     CDS             complement(11125..11658)
                     /gene="nusG"
                     /locus_tag="VGC69_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY0293864.1"
                     /translation="MSKRWYVVHVYSGMEKSVQKALNERIERAALQTSFGRILVPSEE
                     VVEMKGGQKSISERRIFPGYVLVEMDLTDESWHLVKNTNRVTGFLGGSGNRPSPISEK
                     EVEKILNQMEEGVEKPRPKVLFEVGEMVRVKEGPFADFNGNVEEVNYEKSKVRVSVTI
                     FGRATPVELDFSQVEKT"
     gene            complement(11668..12048)
                     /gene="secE"
                     /locus_tag="VGC69_00875"
     CDS             complement(11668..12048)
                     /gene="secE"
                     /locus_tag="VGC69_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY0293865.1"
                     /translation="MSNTSVETVTSTSDRIKLGLAVLVIIAGIVGFSVLGSQPMAARV
                     GVFVGGLIIAALIAWFSEPGRRTLSFGNESYNEVRKVAWPSRKETVQMTGIVFAFVAI
                     MGAFMWVLDKGIEWVLYGLLLGWK"
     gene            complement(12130..12205)
                     /locus_tag="VGC69_00880"
     tRNA            complement(12130..12205)
                     /locus_tag="VGC69_00880"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12170..12172),aa:Trp,seq:cca)
     gene            complement(12382..>12474)
                     /locus_tag="VGC69_00885"
     CDS             complement(12382..>12474)
                     /locus_tag="VGC69_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY0293866.1"
                     /translation="APIAMEEGLRFAIREGGRTVGAGVVAKIIA"
BASE COUNT         2017 a         3816 c         4088 g         2553 t
ORIGIN      
        1 aaaagcggcg ccggaaacat cccggcgccg ccttgtctat tgctgcctat tcccaaccgg
       61 caggcaacca gccgaattac tcggcgctgt cgttctcgct gccgtgatca tcgtccgggt
      121 tgccgaaaat ggccacagtc tgggctgccg tatcggcggc acgggccgct ttttccgatt
      181 ccagcaaggc ggtacgctct tcggcttccc atgcgtcctt ttccttgcgg gccaagtggt
      241 aagccaggcc ggtacccgcc gggatgaggc ggccgacgat gacgttttcc ttcaagccgc
      301 gcaggtcgtc gcgcttgccc atgatggccg cttcggtcag cacgcgggtg gtttcctgga
      361 aggaagccgc ggagatgaac gaatcggtcg acagcgaggc cttggtgatg cccagcagca
      421 cgttctcgaa tatggccggg cgcttctcct cggccacgac gcgatcgttc tcgttgagca
      481 gttccgaacg ctcgacctgc tcgccggtga tgaacgacgt gtcgccggca tcgacgatgt
      541 tcacgcggcg cagcatctgc cgcacgatca cttcgatgtg cttgtcgttg atcttcacgc
      601 cctgcagacg gtagacgtcc tgcacttcgt cgacgatgta ggtggcgagc ttctcgatgc
      661 cctgcaggcg caggatgtcg tgcgggtcgg cggggccgtc gacgatcatt tcgcccttgt
      721 tcaccacttg gccgtcgtgc accagcacct gcttttcctt cggaatcagg aactcgtggc
      781 tggcgccttc catgtcggtg atgaccaggc gctgcttgcc cttggtgtcc ttgccgaacg
      841 agaccgtgcc ggtgacttcg gccagcatgc cggcgtcctt gggcgagcgg gcttcgaaca
      901 gttcggccac gcgcggcaga ccgccggtga tgtcgcgggt cttctgcgat tcctgcggga
      961 tacgggcaag cacttcgccc acggcgacct gctggccgtc gcgcaccgtg atcagcgcgc
     1021 ccaccgggaa cgagatgttc acggtatggt cggtgccggc gatcttgacg tcctcgcccg
     1081 catcgttgac cagcttgatc tgcggacgca tggccacctt gccgccgcgc gtcttgggcg
     1141 tgatgacgac cagggtcgac aagccggtga cttcatcgac ctgcttggcc accgtggcgc
     1201 cttcctcgat gttctcgaag cgcaccgcgc cgccgtattc ggacacgatc ggacgagtca
     1261 gcggatccca gctggccagg cgcgcgccag ccttgacgac ctcgccatcg cccaccagca
     1321 ccgtggcgcc gtaggggatc ttgtggcgtt cgcgttcgcg gccgttgtcg tcgaagatgg
     1381 ccagttcgcc cgagcgcgag atcgccacgc gttcgccctt ggcgttggtg acgtagcgca
     1441 tcgtgccggt gaagcccacg gtaccggccg acttggtttc caccgcgctg gccagggccg
     1501 aacgcgacgc cgcgccgccg atgtggaagg tacgcatcgt gagctgcgtg cccggttcgc
     1561 cgatcgactg ggccgcgatg acgccgaccg cttcgccggt gttgaccttg tagccgcggc
     1621 cgaggtcgcg accgtagcag tgcgcgcaca ggccatggcg ggtttcgcag gtcagcggcg
     1681 tgcggacctt gacttcgtcc acgcccagct tgtcgatggc gtcgaccagg tcctcgtcga
     1741 gcagggtgcc ggcggcgatc gccgtttcct gcgtgtcggg gttgacgatg tcggtggcgg
     1801 ccacgcggcc caggatgcgg tcgcgcagcg gttcgatgac ttcgccgccc tcgaccaggg
     1861 ctttcatgaa gtagccctgc gaggtgccgc aatcgtcttc ggtgatgacc aggtcctgcg
     1921 tcacgtcgac caggcggcgg gtcaggtagc ccgagttggc agtcttcagc gccgtatcgg
     1981 ccagaccctt gcgggcgccg tgcgtcgaga tgaagtactg cagcacgttc aggccttcgc
     2041 ggaagttcgc ggtgatgggc gtctcgatga tcgagccatc gggcttggcc atcaggccgc
     2101 gcatgccggc cagctggcgg atctgggcgg ccgaaccgcg cgcgcccgag tcggccatca
     2161 tgtagatgga gttgaaggac tcctggcgaa cgttctcgcc ctggcggttg accaccggct
     2221 cggtggccag ctgctccatc atggccttgc cgaccttgtc gccggccttg ccccagatgt
     2281 ccacgacgtt gttgtagcgc tcttgcgaag tgaccaggcc cgacgagtac tgcttgtcga
     2341 tttccttcac ttcgcggctg gcctcggcca ggatgccttc cttggccttg gggatcagca
     2401 tgtcttccat ggcgatcgaa atgccgccgc gcgtggccag gcggaagccc gactgcatca
     2461 gcttgtcggc gaagatcacc gtgtcgcgca ggccgcaacg gcggaacgac tggttgatca
     2521 ggcgcgagat ttccttcttc ttgagcgcct tgttgagcac cgtgaaaggc aggcccttgg
     2581 gcaggatttc ggacagcagc gcgcggccga ccgtggtttc atggcggcgg atgaccggct
     2641 gccactcgcc gttggcgtcg cgctcgtgtt ccttcaggcg cacggtgatg cgggtctgca
     2701 gggtgacttc gttgttgtcg taggcgcgct gcacctcggc cacgtcggtg aagaagatgc
     2761 cttcgccctt gccgttgatg cgctcgcggg tcgtgtagta caggcccagc acgatatcct
     2821 gcgaaggcac gatcgagggc tcgccgttgg ccgggaacag cacgttgttc gaggccagca
     2881 tcagggtgcg ggcttcgagc tgggcttcca gcgacagcgg cacgtggacg gccatctggt
     2941 cgccgtcgaa gtcggcgttg aacgccgcgc aaacgagcgg gtgcagctgg atggccttgc
     3001 cttcgatcag ggtcggctcg aacgcctgga tgcccaggcg gtgcagcgtc ggcgcgcggt
     3061 tgagcatgac cgggtgttcg cggatcacct cttcgaggat gtcccacacc accggttcct
     3121 ggctttccac cagcttcttg gcggccttga tggtcgtggc caggcccatc atctcgagac
     3181 gattgaagat gaagggcttg aacagttcca gcgccatcag cttgggcagg ccgcactgat
     3241 gcagcttgag ctgcgggccc accacgatga ccgaacggcc cgagtagtcg acgcgcttgc
     3301 ccagcaggtt ctggcggaag cggccgctct tgcccttgat catgtcggcc agcgacttga
     3361 gctggcgctt gttggcgccg gtcatggcct tgccgcggcg gccgttgtcg agcagcgagt
     3421 cgacggcttc ctgcagcatg cgcttttcat tgcgcagaat gatttcgggc gccttcagtt
     3481 ccagcaggcg cttgaggcgg ttgttgcggt tgatgacgcg gcgatacagg tcgttcaggt
     3541 cggacgtggc gaaacggccg ccgtccagcg gcaccagcgg acgcaggtcg ggcggcagca
     3601 ccggcagcac ttccatgacc atccactcgg gcttgatgcc cgacttctgg aagccttcca
     3661 gcaccttcag gcgcttggag atcttcttga tcttggcttc cgagctggtg gccttgagct
     3721 cggcgcgcag ggtctcgact tcgcggtcga tgtcgatggt gcgcagcagt tcgcgcacgg
     3781 cctcggcgcc catcagggcg cggaagtcgt cgccgtactc ctcggtcttg gcgaggaaat
     3841 cgtcatcgga catgatctgg ccgcgcttga gcggggtcat gccgggttcg atcacgcacc
     3901 aggcttcgaa gtacagcacg cgttcgatgt cgcgcagcgt catgtcgagc accatgccca
     3961 ggcgcgaggg caggctcttc aggaaccaga tgtgcgcgac ggggctggcc agctcgatgt
     4021 ggcccatgcg ctcgcggcgc accttggcga cggtgacttc gacgccgcac ttttcgcaga
     4081 tcacgccacg gtgcttgagg cgcttgtact tgccgcacag gcactcgtag tccttgatgg
     4141 gcccgaagat cttggcgcag aacaggccat cgcgctcggg cttgaaggta cggtagttga
     4201 tcgtctcggg cttgcggact tcgccgtagg accacgaacg gatcttctcg ggcgaggcga
     4261 tgccgatctt gatggcatcg aattgctcgt cttgagagac ttgcttgaag aggtcgagta
     4321 acgctttcat cagttacgct ccaaatccat gtcgagggcc agggaacgga tttccttgac
     4381 gagcacgttg aacgattccg gcatgccggc atcgatgacg tgatcgccct tgacgatgtt
     4441 ttcgtaaacc ttggtacggc cggcgatgtc gtcggatttc accgtcaaca tttcctgcag
     4501 cgtgtacgaa gcaccgtagg cttccagcgc ccacacttcc atttccccga aacgctggcc
     4561 accgaactgc gccttgccgc ccagcggctg ctgggtcacg agcgagtacg gaccggtcga
     4621 acgggcgtgc atcttgtcgt cgaccaagtg gtgcagcttc aggtagtgca tatagccgac
     4681 cgtgacggga cgctcgaact tctcgccggt gcggccgtcg aacagccagg cctgcgcgcg
     4741 cgagggcgtg agctgcatgc gctcggcgat ctcgtcgggg taggccagct tgagcatcgc
     4801 gccgatttct tcctcggtgg cgccgtcgaa gacgggcgtg gccaggggca cgccgtccct
     4861 caggttgtgc gccatttcga tgacttcgtc gtcggtcagc tcatcgatgc gcgcgccggt
     4921 gccggtcgag ttgtagacct tttccaggta ggcgcggacg ttcttgacct gcgccgtgcg
     4981 ctcgtcgcgc agcaagtcgg cgatgcgctg gcccacgccc ttggcggccc agcccaggtg
     5041 gacttccagc acctggccga cgttcatccg cgagggcacg cccagcgggt tcagaacgat
     5101 gtcggcggtg gtgccgtcgg ccatgtgggg catgtcctcg accggggtga tgcgcgagac
     5161 cacgcccttg ttgccgtggc ggccggccat cttgtcgcca ggctgcaggc ggcgcttgac
     5221 ggccaggtac accttgatca tcttgagcac gcccggcggc agttcgtcgc cctgcgtgag
     5281 cttcttgcgc ttttcttcga aggccaggtc gaactggtgg cgcttctgct cgagcgactc
     5341 cttggcctgt tccaggacga cggcgtgctg ctcgtcggcc aggcggatat cgaaccactg
     5401 ccagcggtcc atgtcggcca ggtaagcctt ggtgacgatg gcgcccttgg ccagcttctt
     5461 cggaccgcca ttgacggtct tgccgttgag catcttctcg agacggtcga actggtcgtt
     5521 ctcgacgatg cgcagctggt cgttcaggtc ctggcgatag cggcgcagtt catcgtcgat
     5581 gatggactgg gcgcgcttgt cgcgcacgat gccttcgcgg gtgaacacct gcacgtcgat
     5641 gacggtgccg accatgcccg acggtacgcg cagcgaggtg tccttcacat cggaagcctt
     5701 ctcgccgaag atcgcgcgca gcagcttttc ttccggcgtg agctgggtct cgcccttggg
     5761 cgtgaccttg cccaccagca cgtcgtcggc gctgacttcg gcgccgatgt agacgatgcc
     5821 cgaatcatcc agccggttga gctgggtttc ggccaggttg ctgatgtcgc gggtgatttc
     5881 ctcgggtccg agcttggtgt cgcgggcaac cacggtcagc tcttcgatgt gcaccgacgt
     5941 atagcggtcg tcggccacga ccttctcgga gatcaggatc gagtcttcga agttgtagcc
     6001 gttccagggc atgaacgcga tcagcatgtt ctggcccagg gccagttcgc ccaggtcggt
     6061 cgatgcgcca tcggccagca catcgccctt ggcgacacgg tcgccgcgct tgacgatggg
     6121 acgctggttg atgttggtgt tctggttgga acgggtgtac ttgatgaggt tgtagatgtc
     6181 cacgcccact tcgccggcga cgttctcgtc gtcgttgacg cggatcacca cgcgctcggc
     6241 gtcgacgtgg tcgaccacgc cgccgcgcag ggcctgcacg gtggtgccgg agtcgacggc
     6301 cacggtgcgt tcgatgccgg tgccgaccag cggcttctgc ggacgcgggc aaggcacggc
     6361 ctggcgctgc atgttggcgc ccatcaacgc gcggttggcg tcgtcgtgtt ccaggaacgg
     6421 aatcagcgag gccgccaccg agacgatctg cgacggcgcc acgtccatgt agtgcacgtt
     6481 cgacggcgcg gtcatcatgg tttcgccggc ttcgcggcaa gccacgaggt cgtcgacgaa
     6541 acggccttct tcgtcgagct cggcgttggc ctgggcgatg acgtagtggc tttcctcgat
     6601 ggccgacagg tagtcgatct ggtcgctcac cttgccgtcg atgatcttgc gatagggcgt
     6661 ttccaggaag ccgtactcgt tcaggcgcgc gtacagcgcc atggagttga tcaggccgat
     6721 gttcgggcct tcaggcgttt cgatcgggca gacgcggcca tagtgcgtgg ggtgcacgtc
     6781 gcgcacttcg aagccggcgc gctcgcgggt caggccgccg gggcccaggg cggaaacgcg
     6841 gcgcttgtgc gtgatttccg acagggggtt ggtctggtcc atgaactgcg acagctggct
     6901 cgaaccgaaa aactccttga tggcggccga gatcggcttg gagttgatca ggtcgtgcgg
     6961 catcaggttt tcggtttcgg cctggccgag acgctccttg accgcgcgtt cgacgcgcac
     7021 caggccggcg cggaactggt tctcggccag ttcgcccacg caacgcacgc ggcggttgcc
     7081 caggtgatcg atatcgtcga tctggccgcg gccgttgcgc agctcgacca gcaccttgat
     7141 ggtctcgagg atgtcgtcgt tggtcagcgt catcggaccg acgttggtgt cgccgcggcc
     7201 cagccggctg ttgaccttca tgcgtcccac gcgcgagaga tcgtaggtct cttcgctgta
     7261 gaacaggcgc tggaacagag cctcgaccgc ttcttcggtc ggcggctcgc cggggcgcat
     7321 catgcggtag atggccacgc gcgcggccat ctggtcgacg gtttcgtcgg tgcgcagggt
     7381 ctgcgagatg tagggaccgc gatccagatc gttggtgtac agggtctgga agccggcgac
     7441 cttggcttcg cgcatggccg acagcaccga ttcggtgatc tcgtcattgg cgtgggccac
     7501 gatttcaccc gtttcggcgt cgacgatgtt cttggccagc acgcggccga acaggaagtc
     7561 ctcgggcacc gaaatgcgct cgatgccggc ggtggccatg tcgcgcagat gcttggcgtt
     7621 gatgcgcttg tccttttcga cgagcacctt gccggagcgg tcggagatgt caaagcgcgc
     7681 gatctcgccc ttccagcgct cggacaccac ttccatcatg gcgccttcgt tctgcagctc
     7741 gaagttgtcg aagtcgaaga acccgcccag gatggtctcg ggcgtcatgc cgatggcctt
     7801 gagcaggatc gtgacgggca tcttgcgccg gcggtcgacg cggaagaaga gaacgtcctt
     7861 gggatcgaac tcgaagtcca gccacgaacc gcggtaggga atcacgcggg cggagaacag
     7921 cagcttgccc gagctgtggg tcttgccgcg gtcgtgctcg aagaacacgc cgggcgagcg
     7981 gtgcagctgc gacacgatga cgcgctcggt gccgttgatg acgaaggagc cggtgctggt
     8041 catgagcgga atttcgccca tgtagacttc ctgttccttc acttccttga tggtgggttt
     8101 gctgacctcg cggtccatca gcaccaggcg caccttggcg cgcaggggcg aggcgtaggt
     8161 caggccgcgt tgctggcatt ccttgacgtc gaacaccggt tcgcccagca cgtagctgac
     8221 gaactccaag cgcgccatac cgttatggct gacaatcggg aaaatcgacg aaaacgccgc
     8281 ttgcaggcct tcgctggccc gtcccgcgga gggtacgtcg gattgcagga acgttaggta
     8341 ggattgaagc tgagtcgcca gcaggaaggg cacgttctga acgtcttcac gcttggcgaa
     8401 gcttttgcgg atgcgctttt tttcggtgta cgagtaaggc atgagcactc cgactcgagg
     8461 ttgcatgggc cgtcaaccac ggccctaggt gttacgactc caggaaacgc ttctgcgcgg
     8521 ccggctcggt gggtaccggg cctaaaaaca cctttcgcaa caagggtttc ctggaaacgc
     8581 aaaagcccgg ggacggcaaa atgcgctatc cccgggcagc ttggacgaag tcttacttga
     8641 cttcgacctt ggcgccggct tcttccagct tcttcttggc ggcttcggcg tcagccttgg
     8701 caacgccttc cttcacgggc ttgggagcgc cgtcgaccag gtccttggct tccttcaggc
     8761 cgaggcccgt cagttcgcgc acggccttga tgacgctgac cttgttcgcg ccggtttcag
     8821 tcagcacgac ggtgaactcg gtctgctctt cggcaacggc ggcagcgccg ccggcggcgg
     8881 gagcggccac agccacggcg gcggcggcag ccgacacgcc aaacttctct tccagatcct
     8941 tgatcagctc ggacagctcg agcacggtca ggccagcgat ggcgtcaagg atttcagctt
     9001 tgttaagtgc catttttcag aactccaaaa tatttggtta atgccaggtt ggttgtcgct
     9061 tggtcgttca gcgaatttcc gaaccgtcgc ccattcaggc ggcagccttc tggtcgcgca
     9121 cggcagcgag gccgcgggca aacttggtcg gaacttcgtt gagcgtacgc acgaattgcg
     9181 cgatcggggc ttgcatggtg cccagcagct tcgagagcag ttcttcacgc gagggcaggg
     9241 aggccaaagc cttcacgcct tcggtgttca gcacgttgtt gggcagcgaa ccgcccttca
     9301 ggacgagctt gtcattgctc ttggcaaagc cggcgagtac cttggccgcc gcgaccgggt
     9361 cggtgctgat accgtagatc agcggaccgg tcagttgcga ggtcaacggc tcgaaagccg
     9421 tgcccgcaac ggcacgacgg gctagcgagt tcttgagaac acgcaggtaa acgcccgatt
     9481 cacgcgcagt tttgcgcagt acggtgacgg aggcgacgtc cagaccacga tactcagcga
     9541 cgatgatcga ttgggccttg gcaacttgcg ccgcgacttc ttcgattacg accgccttct
     9601 cttgacgatt gagactcacg gtttgaacac tccatcaaaa gacgcctggg gcttggtgct
     9661 gcttgtaagc cttgcgcgtc aaccgaatgc ggcgcctggt tgagttcttc gggtaaagaa
     9721 ttcttcctgg gacaccgtct acgctggatc cggaacgtcg ggttgcccct gggtttcggt
     9781 attaagcacc ggccccgata cagcctgggg aaggcgctcc agcggtcttt gacagctgca
     9841 cccggaaaat ccggatgcgg cccaaagtac gttacgtgct ggcggcgatc aggccgacag
     9901 cgaggcaatt tccacgcgcg caccgccgcc catggtggac gacacggcga gcttgcgcag
     9961 ataaatgccc ttggcagcag cggggcgagc cttttgcagg gcgtcgacca gcgcggccag
    10021 gttggtttgc agctgttcca cgccaaacga ggcgcggccg atggtggcgt gaatgatgcc
    10081 ggccttgtcg gtgcgatatt gcacctgacc ggccttggcg ttcttcacgg cagtggcgac
    10141 atcgggggtc acggtgccga ccttggggtt cggcatcagg ccgcgcgggc ccaggatctg
    10201 gcccagcgta ccgacgatac gcatggtgtc gggcgaggcg atgaccacgt cgaagttgaa
    10261 ggtgccgccc ttgatgctct cggccaggtc ttccatgccg acgatgtcgg cgccggcggc
    10321 gcgagcggcc tcggccttgt cgccctgggc gaacacggcc acgcggacgc tcttgccggt
    10381 accggcaggc atgaccaccg agccgcggac cagctggtcg gacttcttgg ggtcgatgcc
    10441 gagctgcacg gccacgtcga tggattcatc gaacttggcg gtggcggttt ccttgaccag
    10501 ggtcagggct tcggcgaccg ggtagaactt gttgcggtcg atcttcgcag cgatggcgac
    10561 ggcgcgcttg gagagttttg ccatgatcag atcccctcga cggtgatgcc catgctgcgg
    10621 gcgctgccag cgatcgtgcg gacggcggcg tcgagatcgg ccgcggtcag atcgggttgc
    10681 ttggtcttgg cgatttcttc agcctgggcg cgggtgagcg tgccgacctt gtcggtatgc
    10741 ggcttgggcg aacccttttg cacgccggcg gccttcttga tgaggaccgt cgcgggcggg
    10801 gtcttcatga tgaaggtgaa gctcttgtcg gcgaacgccg tgatcaccac gggaatcggc
    10861 aggccgggct ccaggccctg cgtcttggcg ttgaacgcct tgcagaattc catgatgttc
    10921 aggccgcgct gacccagggc cgggccgatc gggggggagg ggttcgcctt accagccggc
    10981 acttgcagct tgataaagcc gacgatcttc ttcgccatgc tttgctcctt gcgggttcta
    11041 gcgctcgccg gtcttggcgg gctccccggg gttaataaag gtactgctta atgggtacag
    11101 ccatcggggt actgctactg ccagtcaggt cttttcgacc tggctgaaat ccagttccac
    11161 cggcgtggcg cggccaaaga tggtgaccga tacacgcacc ttgctctttt cgtagttgac
    11221 ttcttcgacg ttgccgttga agtccgcaaa cgggccttcc ttcacgcgaa ccatctcgcc
    11281 cacctcgaac agcaccttgg ggcggggctt ctcgacaccc tcttccatct ggttgaggat
    11341 cttctcgact tccttttcgg aaatcggcga gggacggttg cccgagccac ccaggaagcc
    11401 ggtgacgcgg ttggtgttct tgaccaggtg ccaggactcg tcggtgaggt ccatctcgac
    11461 caggacatag cccgggaaaa tgcgacgctc gctgatcgac ttctggccgc ccttcatttc
    11521 gacgacttct tccgacggca ccaggatgcg gccgaaagac gtctgcagtg cggcgcgctc
    11581 gatgcgctcg ttaagcgcct tctggacgct tttttccatg ccggaataga catggacgac
    11641 ataccaacgc ttactcatga cggcccttta tttccagccc agcaacaggc cgtagagtac
    11701 ccactcgatg cccttgtcga gcacccacat gaacgcaccc atgattgcca cgaaggcaaa
    11761 cacgatgccg gtcatctgga cggtttcctt gcggctgggc caggcaacct tccgaacttc
    11821 gttgtaggac tcgttgccga agctcagagt gcggcgaccg ggctcgctga accaggcgat
    11881 gagcgcggcg atgatcaggc cgccgacgaa cacgccgacg cgggcggcca tgggctgcga
    11941 gcccaacacc gaaaaaccga cgatcccggc aatgatgacg agaaccgcca atccgagctt
    12001 gatccggtcg gaagtgctgg ttacggtttc tacgctggtg ttagacattt caagaacgag
    12061 tcgaggccgg aaactgctgg gccactgggt ttgcttggcg gctggcttgg caatactgct
    12121 gggaggactt ggcaggggca gtaggaatcg aacctacaac cttcggtttt ggagaccgac
    12181 gctctgccaa ttgagctata cccctacggc agttgaacct tcacaacagt caaccatgca
    12241 tggcgccttg cggaaccaag cataatacta cttttatggc caagatggaa atagggcgga
    12301 tttggccctt tttcttttgt tgcaaatgcc cggaccagcc gggcatttgc agtgcgggcc
    12361 caaggcccgc ctcatcgagg tttaggcgat gattttagcg acgacaccgg cgccgacggt
    12421 acgcccgcct tcacggatgg caaagcgcag gccttcttcc atcgcgatcg gcgc
//
LOCUS       DASWPO010000021         8042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1431692, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000021 DASWPO010000000
VERSION     DASWPO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431692"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..817
                     /locus_tag="VGC69_00890"
     CDS             50..817
                     /locus_tag="VGC69_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0293867.1"
                     /translation="MSGSALLIQSLEKGLMVLEHFRNADSMNLQEIARTCGITTGSAQ
                     RVAYTLEHAGYLHKDPRSKRYRVTVKAVGLGYNYLYREPLFQHAHVVLHQINQECGEI
                     VNLSVPDDDDNMVFVMRVATARHIPIYLPAGTRIPCVASASGRAYWAALPEAETDARF
                     ATLHTQKHTPSTTTDTARLQALVDEARRNRYAYADEEFYPGDINVAAAVHDGAGRPIA
                     AVNISVPKPRWSLERAREELAPLAIRAARAISRHEAG"
     gene            complement(830..1600)
                     /locus_tag="VGC69_00895"
     CDS             complement(830..1600)
                     /locus_tag="VGC69_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0293868.1"
                     /translation="MPESPRRKPARLVLEPAQRTELLRVQRRLLLKSGLSLGALSMLA
                     GCNMQDGDTVDKVLWAMSRWNDRVQGWLFRGARLAPTYTAAQVTDPFPFNAFYPEYNV
                     PDIDLSTYKLEVSGMVADKRDWTLEALRRLPQAGQITRLICIEGWSAIGQWSGVPLKA
                     FLQAVGADLTARYVGFKCADRYYSSLDMATALHPQTILALDFGGQALPPDFGNPLRLR
                     VPTKLGFKNPKHIMSIFVTNAFPGGYWEDQGYNWFSGI"
     gene            complement(1593..2267)
                     /locus_tag="VGC69_00900"
     CDS             complement(1593..2267)
                     /locus_tag="VGC69_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY0293869.1"
                     /translation="MGSAVSGPPASGRGRARIRVHALPVRLSHWLNVYAMVCMFMSGW
                     GIYNASPIFGFEFPVWATLGGWLGGSTSWHFAVMWLLVANALAYLLWGLASGHLRRDL
                     LAVTPRAVVRDMVAALRFKLGHEVGRYNAVQKAMYLGVLLLGALIVLSGLAIWKPVQL
                     HELTDFFGGFAAARVVHFAAMSGIALFLLVHLALVLIVPSTLPPMITGWARAHRKGDH
                     DGAGDA"
     gene            complement(2280..2897)
                     /locus_tag="VGC69_00905"
     CDS             complement(2280..2897)
                     /locus_tag="VGC69_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293870.1"
                     /translation="MHFTSRTLVAAAVMAVAASAHAQPKPPARLVVRGTISQVTATSL
                     EVAQRNGQKATVALPADAAIRVVSNAKLSDIQPDSFIGTAASPQPDGTLKALEVHVFA
                     ASLRGSGEGFRPWEGADGKAGTMTNGTVGSLVNSNGTTMTVKYKEGEKTIVVPPDVPI
                     VYMEPGDRSLLKTGVPVLIFPTKDAQGGLVANSVIAGKNGVIPPM"
     gene            complement(3011..3577)
                     /locus_tag="VGC69_00910"
     CDS             complement(3011..3577)
                     /locus_tag="VGC69_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY0293871.1"
                     /translation="MATPQIHARFTPMQRMLHWLMAICILAMLFIGVGMVSTVTAKYL
                     TLVSIHKPLGIAILILAVLRLGLRLKAGAPALPRDLPAPIRLAAKLSHVLLYALMIAM
                     PLLGWGMLSAADYPVALIGGYRLPAILPQSDALHTVLWDAHRVLAFAFFALILAHIAG
                     ALFHALVRRDGVFWAMAPVSGRSGTRRE"
     gene            complement(3577..4629)
                     /locus_tag="VGC69_00915"
     CDS             complement(3577..4629)
                     /locus_tag="VGC69_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HEY0293872.1"
                     /translation="MPQPSTRSNIGSLLVIAVVVAGGAAAFAYTAGWLSPGRVTPDRL
                     VDRLAPPTGPVLGYRRNHAKGVCFTGAFEANGAGAALSDARVFAAGRYPVMGRFNVAG
                     PIPTADDATARVRGMGLQIKTPDGQEWRSATIDVPFFPVGTVQAFYDLQSASATKDAP
                     DAVKRVVAAHPEIAVFGAWAKSAPFAPSFADDRYNSLNSFVFTDAAGNSRVVRWSLVP
                     RTQPTLVSADDMKARGPDFLKGEIAARVAAQPQRWGLFVTVAEPGDPTADPSKAWPAS
                     RRAVDVGTLVVDRVLPEPDGPCRDINFDPTVLPPGIGTSDDPFPAARSAAYAVSYNRR
                     TAEAGHYPRTMTESSK"
     gene            4796..5482
                     /locus_tag="VGC69_00920"
     CDS             4796..5482
                     /locus_tag="VGC69_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEY0293873.1"
                     /translation="MRILVVEDDTRTGEYLKKGLGESGYAVDWAQHGADGLHMALEHA
                     YDLIILDVMLPGMDGWQIMQTLRRRHDTPVLFLSARDHLDDRIRGLELGADDYLVKPF
                     SFTELALRVRTLLRRGVTREADQFHLADLHIDVLRRRVTRMNAPIALTNKEFTLLHLL
                     VRREGEVLSRTLIASQVWDMNFDSDTNVVDVAIKRLRAKIDRAFDPKLIHTVRGIGYV
                     CEVRPCDPAP"
     gene            5464..6870
                     /locus_tag="VGC69_00925"
     CDS             5464..6870
                     /locus_tag="VGC69_00925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEY0293874.1"
                     /translation="MRSRSLTLRAALLFALVAALVVSAAGVYLYASMRVTMIGRGDTG
                     LVGRVERFRALLPDTLRLDELRARPVLFENMLGNEQDVLVFSPPGQPPVVNVNPLGEP
                     LPQFAPVPIDQPLSLADVRDAPTREGGHMRVVAALARSADGTLLQIQAAHLLASETHM
                     LARYRGQIAAAVLAAFLSIALLGYVTLRRALRPLRAMAVQASRITPASLDTRLSLADT
                     PHELRQLTASFNAMLERLADGYRRLTQFSADLAHEIRTPIGALMGNFQVALYQRRAPE
                     EYENLLATGLEELERISRLIENILFLARADDAQAALAYAELDLPAELGKVAEYFEGLA
                     EERGIRIEYKARGTLRADPMLFRRALGNLVANAVRYADADSVVRLAASAEPGAVLVGV
                     DNQGPPIPSERLGKLFDRFYQGDAARSAASDSSGLGLAIVRAIMTLHDGSAAAENTAG
                     GRVRFTLRYPTPGEPHDA"
     gene            complement(6882..>8042)
                     /locus_tag="VGC69_00930"
     CDS             complement(6882..>8042)
                     /locus_tag="VGC69_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0293875.1"
                     /translation="AEARGEVDFAASFLVHAAEAGRRLQGEILPGDAPEEQIWIQRVP
                     YGVVAAMTAWNFPVALFTRKVGPALIAGNAIVIKPHELTPLTSLALAELAARAGVPAG
                     VLNVVTGLGREAGHRLATSPRADLVSMTGSVRAGREIYAAGSADIKPIRLELGGKAPF
                     IVLADADLDRAAEAAVFSKFLNGGQVCTANDRMYIERGVYDAFLDKLLARVRGLRVGN
                     PFEGADIGPRVSAPEVGKLQGILDGALAEGARGLVDFNRGGHGDFYARGNWFFPAVLA
                     VPSNGLRIMREETFGPILPVMPVDSFDEALCLANQCDYGLSAYLFTASNRNIMRCVAE
                     LAFGEIYVNRGAGESVHAFHAGYKLSGIGGEDGKHGIEGYLRKKTLYNRYAA"
BASE COUNT         1335 a         2861 c         2666 g         1180 t
ORIGIN      
        1 ccgacctgcc acatgcggtc aatgcgtatc aaccataggg taatgccgca tgagcggttc
       61 tgcattgctg atccaatcgc tggaaaaagg cttgatggtg ctcgagcact tccgtaacgc
      121 cgacagcatg aacctgcagg aaatcgcccg gacctgcggc atcaccaccg ggtcggcgca
      181 gcgcgtggcc tacacgctgg agcacgccgg ctatctgcac aaggacccgc gcagcaagcg
      241 ctaccgcgtc accgtcaagg ccgtgggcct gggctacaac tacctgtatc gcgagccgct
      301 tttccagcac gcccatgtgg tgctgcacca gatcaaccag gagtgcggcg agatcgtcaa
      361 cctgtcggtg ccggacgacg acgacaatat ggtgttcgtg atgcgggtgg ccacggcccg
      421 gcacatcccc atctacctgc ctgccggcac gcgcatcccc tgcgtggcct cggcctcggg
      481 ccgcgcctat tgggccgccc tgccggaagc ggaaaccgac gccaggttcg ccacgctcca
      541 cacgcagaaa cacacgccgt ccaccacgac cgacacggcg cgcctgcagg cgctcgtcga
      601 cgaagcgcgc cgcaatcgct acgcctatgc cgacgaggaa ttctatcccg gcgacatcaa
      661 tgtggccgcc gccgtgcacg acggcgccgg gcggcccatc gccgcggtga acatctcggt
      721 gcccaagccg cgctggtcgc tcgagcgcgc ccgcgaagag ctcgcgccgc tggccatccg
      781 cgcggcgcgg gccatcagcc ggcacgaggc cggctagggc ccgccgcgct cagatgccgc
      841 tgaaccagtt gtagccctgg tcttcccaat agcccccggg gaaggcattg gtcacgaaga
      901 tcgacatgat gtgcttgggg ttcttgaagc ccagcttggt cggcacccgc agccgcagcg
      961 ggttgccgaa gtccggcggc agcgcctggc cgccgaagtc cagcgccagg atggtctgcg
     1021 gatgcagcgc cgtggccatg tccaggctcg agtaatagcg gtcggcgcac ttgaagccca
     1081 catagcgcgc cgtcaggtcg gcgccgaccg cctgcaggaa ggccttgagc ggcacgccgc
     1141 tccactggcc gatcgcgctc cagccctcga tgcagatcag ccgcgtgatc tgcccggcct
     1201 gcggcagccg gcgcaaggcc tccagcgtcc agtcccgctt gtcggccacc atgccggata
     1261 cctccagctt gtacgtcgac aggtcgatgt cgggcacgtt gtattcggga tagaaggcat
     1321 tgaacggaaa cgggtcggtg acctgggcgg ccgtataggt cggcgccagg cgcgcgccgc
     1381 gaaacagcca gccctgcacc cggtcgttcc agcgcgacat ggcccacagc actttgtcga
     1441 ccgtgtcgcc gtcctgcata ttgcagccgg ccagcatcga cagcgcgccc agcgacaggc
     1501 cggacttcag cagcagccgg cgctgcacgc gcagcagctc ggtgcgctgg gccggctcca
     1561 ggaccaatcg cgcaggcttg cggcgtgggg attcaggcat cgccggctcc atcatgatcg
     1621 cccttgcgat gcgcgcgcgc ccagccggta atcatgggcg gcagggtcga gggcacgatc
     1681 agcaccaggg ccaggtgcac cagcaagaac agcgcaatgc cggacatcgc cgcgaaatgc
     1741 accacgcgcg ccgcggcgaa gccgccgaag aaatcggtga gttcgtgcag ttgcaccggc
     1801 ttccagatcg ccaggcccga cagcacgatc agcgcgccca gcagcagcac gcccaggtac
     1861 atggcctttt gcacggcgtt gtagcggccg acctcatggc ccagcttgaa gcgcagcgcc
     1921 gccaccatgt ccctgaccac ggcgcgcggc gtcaccgcca gcaggtcgcg gcgcaggtgg
     1981 ccgctggcca ggccccacag cagataggcc agcgcgttgg ccaccagcag ccacatcacc
     2041 gcgaaatgcc aggacgtcga gccgcccagc cagccgccca gggtggccca caccgggaac
     2101 tcgaagccga agatcggcga ggcgttgtag atgccccagc cgctcatgaa catgcacacc
     2161 atggcataga cattgagcca gtgcgacagc cgcaccggca gggcatgcac ccgaatccgg
     2221 gcccgcccgc gcccgctcgc cggcgggccg gaaaccgcgc tacccatcgt cggcctcgct
     2281 tacatcggcg gaatgacgcc gttcttgccg gcgatgacgg agttggccac caggccgccc
     2341 tgggcatcct tggtggggaa gatcagcacc ggcacgcccg tcttgagcag gctgcggtcg
     2401 cccggctcca tgtacacgat aggcacgtcg ggcggcacca cgatggtctt ctcgccttcc
     2461 ttgtacttga ccgtcatggt ggtgccgttg ctgttgacca ggctgcccac ggtgccgttg
     2521 gtcatggtgc ccgccttgcc gtcggcgcct tcccagggcc ggaagccctc gcccgaaccg
     2581 cgcaggctgg cggcgaacac gtgcacttcc aacgccttga gcgtgccgtc gggctgcggc
     2641 gaagcggccg tgccgatgaa gctgtcgggt tggatgtcgg acaatttggc gttggacacc
     2701 acgcggatcg cggcatcggc cggcagcgcc accgtggcct tctggccgtt gcgctgggcg
     2761 acttccagcg acgtggcggt aacctggctg atggtgccgc gcaccaccag gcgagcgggc
     2821 ggcttgggct gggcatgggc cgaggcggcc acggccatga cggcggcggc gaccagagtg
     2881 cgggaggtga agtgcatgga actctcctat tgaggtaaga aacctgtcgg catggtcaac
     2941 cttgagccgg gacaggggca tgacatgcga atgacaaagt tgtcatgaac gcgcgcagtg
     3001 aatcgccgga ctattcgcgc ctcgtcccgc ttcggcccga aacgggagcc atggcccaga
     3061 atacgccgtc ccggcggacc agggcatgga acaaggcccc cgcgatgtgc gcgaggatga
     3121 gcgcgaagaa ggcgaaagcc agcacacggt gcgcgtccca cagcaccgtg tgcaaggcgt
     3181 cgctctgcgg caggatggcg ggaagccgat atccgccgat gagcgccacc ggataatccg
     3241 ccgcggacag catgccccaa ccgagaagcg gcatcgcgat catcaaggcg tagagaagga
     3301 cgtgcgagag cttggccgcc agccgtatcg gtgcgggcag gtcgcgcggc aaggccggcg
     3361 cgccggcctt gagccgcagc ccgagacgca gaaccgcgag gatcaggatc gcgatgccca
     3421 gcggcttgtg gatcgaaacc agcgtcaggt acttggccgt caccgtcgag accatgccga
     3481 cgccgatgaa cagcatggcc aggatgcaga tcgccatgag ccaatgcaac atccgctgca
     3541 tcggggtgaa acgggcatgg atttggggcg tggccatcat ttggaagact ccgtcatggt
     3601 gcgcggataa tggccggcct cggccgtgcg gcggttgtag gacaccgcgt aagcggctga
     3661 gcgggctgct ggaaacggat cgtccgacgt cccgatgccg ggcggcagca ccgtcgggtc
     3721 gaagttgata tcgcggcagg ggccgtccgg ctcgggcagg acccgatcga cgacgagcgt
     3781 gccgacgtcg accgcgcgcc ggctcgcggg ccatgccttg ctcgggtcgg cggtgggatc
     3841 gcccggctcg gcgaccgtca cgaacaggcc ccagcgttgc ggctgcgccg cgacgcgcgc
     3901 ggcgatctcg cccttgagga agtccggccc ccgggccttc atgtcgtcgg cggagaccag
     3961 cgtcggctgc gtcctgggga cgagcgacca gcgcaccacg cggctattgc cggccgcgtc
     4021 ggtgaaaacg aaactgttca gcgagttata gcgatcgtcg gcaaagcttg gcgcgaaggg
     4081 agcgcttttc gcccaggcgc cgaagacggc gatctccggg tgggcggcca cgacgcgctt
     4141 gaccgcatcc ggggcgtcct tagtcgcgga ggccgactgc aaatcgtaaa aagcctgcac
     4201 cgtccccacg ggaaagaacg gcacgtcgat cgtcgcgctg cgccactcct ggccgtccgg
     4261 cgttttgatc tgcaggccca tgccgcgcac ccgcgccgtc gcgtcgtcgg ccgtcgggat
     4321 cggccccgcg acgttgaagc gtcccatgac cggatagcgg ccggccgcga agacccgcgc
     4381 atccgacagg gccgctcccg ccccgttcgc ctcgaacgcg ccggtgaagc agacgccctt
     4441 ggcatggttg cggcggtagc cgagcaccgg gcctgtcggc ggagccaggc ggtcgaccag
     4501 ccggtcgggc gtcacccgcc ctggcgagag ccagcccgcc gtgtaggcga aggccgccgc
     4561 cccgccggcc acgacgacgg cgatgacaag gagggatccg atgttggagc gcgttgacgg
     4621 ttgcggcatg ggggtccttt gctatgtcca gcgaaagcga tcggaagtgg tatttggcaa
     4681 acgccatatg ttccgccctg cccggggaca gcggcatgac atgcgaatga caatgttgtc
     4741 atgaatgttc cggagcgaat cgctagaatt ccactatacg gaaagaggga acagcgtgcg
     4801 aatactggta gtcgaagacg acacccggac cggggaatac ctcaaaaagg gcctcggcga
     4861 atccggctat gccgtcgact gggcgcagca cggcgccgac ggcctgcaca tggcgctgga
     4921 acacgcctac gacctcatca tcctggacgt catgctgccc ggcatggacg gctggcagat
     4981 catgcagacg ctgcgccgcc ggcacgacac gccggtgctc ttcctgagcg cgcgcgacca
     5041 cctcgacgac cgcatccgcg gcctggagct gggcgccgac gattacctgg tcaagccctt
     5101 ctcctttacc gagctggccc tgcgcgtgcg caccctgctg cgccgcggcg tgacgcgcga
     5161 ggccgaccag ttccacctgg ccgacctgca catcgacgtg ctgcgccgcc gcgtcacgcg
     5221 catgaacgcg cccattgcct tgaccaacaa ggagttcacc ctgctgcacc tgctggtgcg
     5281 ccgcgagggc gaggtgctgt cgcgcacgct gatcgcctcg caggtctggg acatgaattt
     5341 cgacagcgac accaatgtgg tcgatgtggc catcaagcgc ctgcgcgcca agatagaccg
     5401 ggccttcgac cccaagctca tccataccgt gcgcggcatc ggctatgtct gcgaggtgcg
     5461 cccgtgcgat cccgctccct gacgctgcgc gccgccctgc tgttcgccct ggtggccgcg
     5521 ctggtggtca gcgcggccgg cgtctacctg tatgcctcca tgcgcgtcac catgatcggg
     5581 cgcggcgaca ccgggctggt cggccgagtc gagcgcttcc gcgccctgct gccggacacc
     5641 ctgcgcctgg acgagctgcg cgcccgcccc gtcctgttcg agaacatgct cggcaacgag
     5701 caggacgtgc tggtcttcag cccgcccggc cagccgccgg tggtcaacgt caacccgctt
     5761 ggcgagcccc tgccccaatt cgcgccggtg ccgatcgacc agccgctatc gctggccgat
     5821 gtgcgcgacg cgcccacccg cgagggcgga cacatgcggg tggtcgcggc gctggcccgc
     5881 tccgccgacg gcacgctgct gcagatccag gccgcccact tgctggcgag cgagacgcac
     5941 atgctggccc gctaccgcgg ccagatcgcc gcagcggtgc tggcggcctt tctgtcgatc
     6001 gcgctgctcg gctacgtcac cttgcgccgc gcgctgcggc cgctgcgcgc catggccgtc
     6061 caggcctcgc gcatcacccc ggccagcctg gacacccgcc tgagcctggc cgacaccccg
     6121 cacgagctgc gccagctgac cgcctcgttc aacgccatgc tcgagcggct ggccgacggc
     6181 taccggcggc tgacgcagtt ctccgccgac ctggcccacg aaatccgcac gcccatcggc
     6241 gcgctcatgg gcaacttcca ggtggccctc taccagcgcc gcgcgcccga ggaatacgaa
     6301 aacctgctgg ccaccggcct ggaagagctc gagcgcatct cgcgcctgat cgagaacatc
     6361 ctgtttctcg cccgcgccga cgacgcgcag gccgcgctcg cctacgccga actggacctg
     6421 cccgccgaac tgggcaaggt ggccgagtat ttcgagggcc tggccgagga gcgcggcatc
     6481 cgcatcgaat acaaggcccg cggcacgctg cgcgccgacc ccatgctgtt tcgccgcgcg
     6541 ctgggcaacc tggtcgccaa cgccgtgcgt tatgccgatg ccgacagcgt cgtgcggctg
     6601 gccgcgagcg ccgaacccgg cgccgtgctg gtcggcgtcg acaaccaggg cccgcccatt
     6661 ccttccgagc ggctcggcaa gctgttcgac cgcttctacc agggcgacgc cgcgcgcagc
     6721 gccgcctcgg actccagcgg cctgggcctg gccatcgtgc gcgccatcat gacgctgcac
     6781 gatggctcgg cggccgccga gaacaccgcc ggcggccgcg tgcgcttcac gctgcgctat
     6841 ccgacgccgg gcgaaccgca cgacgcctga cccgcgccgc cttatgcggc gtagcggttg
     6901 tacagcgtct tcttgcgcag gtagccctcg atgccgtgct tgccgtcctc gccgccgatg
     6961 ccgctgagct tgtagccggc atggaaggcg tgcacgctct cgcccgcgcc gcggttgaca
     7021 tagatctcgc cgaacgccag ctccgccacg cagcgcatga tgttgcgatt actggcggtg
     7081 aacagatagg ccgacagccc gtaatcgcac tggttcgcca ggcacagcgc ctcgtcgaag
     7141 gaatccaccg gcatcaccgg caggatgggc ccgaaggtct cctcccgcat gatgcgcaag
     7201 ccgttggacg gcaccgccag cacggcgggg aagaaccaat tgccccgcgc gtagaaatcg
     7261 ccatggccgc cccggttgaa atccaccagg ccgcgcgcgc cttcggccag cgccccatcc
     7321 aggatgcctt gcagcttgcc gacctccggc gcgctcacgc gcggaccgat gtccgcgccc
     7381 tcgaacgggt tgccgacccg caggccccgc acccgggcca gcagcttgtc caggaaggca
     7441 tcgtagacgc cgcgctcgat gtacatgcgg tcattggccg tgcagacctg cccgccgttg
     7501 agaaacttgg aaaacaccgc cgcctcggcc gcgcggtcca ggtcggcgtc ggccagcacg
     7561 atgaacggcg ccttgccgcc cagctccagg cggatgggct tgatgtcggc gctgccggcc
     7621 gcatagatct cgcgcccggc gcgcacgctg cccgtcatcg acaccaggtc ggcgcgcgga
     7681 ctggtcgcca gccggtggcc cgcctcccgt cccagcccgg tcaccacgtt gagcaccccg
     7741 gccggcaccc cggcgcgcgc cgccagttcg gccagggcca gcgacgtcag cggcgtgagc
     7801 tcgtgcggct tgatgacgat ggcgttgccg gcgatcagcg ccggccccac cttgcgggtg
     7861 aacagcgcca ccgggaaatt ccacgccgtc atggccgcca ccacgccgta gggcacgcgc
     7921 tggatccata tttgttcctc cggcgcatcg ccgggcagga tctcgccctg cagtcggcgg
     7981 ccggcttcgg ccgcgtgcac caggaagctc gcggcgaagt ccacttcgcc gcgcgcctcg
     8041 gc
//
LOCUS       DASWPO010000022        20733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_629463, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000022 DASWPO010000000
VERSION     DASWPO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20733
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629463"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2650
                     /locus_tag="VGC69_00935"
     CDS             <1..2650
                     /locus_tag="VGC69_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0293876.1"
                     /translation="LPKKTAANRVRWWTAMLCAGPGWIVPGAAKMLAGAFLAYLALSH
                     GVPGAKAADPTQMYRVAFGYVLSNPAWVLALTTLFVVVSQVKINVTNAYAGSLAWSNF
                     FARVTHSHPGRVVWLVFNVLIAICLMEMGVFDALEHVLTVFAHVALAWIGALVADLVV
                     NKPLGLSPKGIEFRRAYLYDINPVGVGASLFSLVVSLVADLGGFGQTAHALSPFIALL
                     AAFAAAPVIARATGGRYYLARRPASLTTRQGDHGLTCVICANTFETGDMAHCPAYNGA
                     ICSLCCTLDARCHDRCKTNSRLQDQIGEALRRLLPRHLATPLFHNRLAHYLLVLGTMA
                     GGLAAILSLIYYQETVAYASIGADIGVLTDTFTKLFAALVLIGGVVAWWMVLTNESRQ
                     VAQEESDRQTNLLLHEIEAHRRTDAQLQQAKEVAEAANLAKSRFMGGMSHELRAPLNS
                     ILGYAQILQRDPTLPSARREAIDVIHRSGKHLVSLIDGLLDIARIEAGKLRLETTELR
                     LPDFLDQIVKMFHPQTALKGLRFDYLVSSDLPRIVHIDEKRLRQILINLMSNAVKFTR
                     EGGVALRVGYASDMVTMVVEDSGAGIPAEDFERIFMPFERSWAAIGQADTGTGLGLTI
                     CRMLTNIMGGELTVKSEVGRGSIFTLKLFLPEVRSSSGSLRPAGLVTGYAGPRRGVLV
                     VDDQPSQRRVLRDMLEPLGFVVHEAPDGHACLLRAADLRPDLILMDISMPNMPGDEAA
                     RMLRQQGVATPVIIVSANVYSLGPHDATTASDWLFKPVVLDELLAKIGAHLKLQWIVQ
                     PEAEESGPADLSGITLAREDAESLLELGAMGYIKGIMARLDDIRDRAPKAAPLVDHLA
                     SLAKEFRLTEYNSLLKDHVRRHFTTD"
     gene            2622..3566
                     /locus_tag="VGC69_00940"
     CDS             2622..3566
                     /locus_tag="VGC69_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0293877.1"
                     /translation="MYDAISRPTEGKLVMLVDDAPDNLKMLSDALDQAGYMVLVATDG
                     ASAIERMDFVLPDIVLMDAIMPGLDGFETCRRLKLHRSAAHVPVVFMTGLTEPENVVR
                     GFQAGGIDYVTKPIDADVVLARLDAHLRTARMMSAAMNAMDATANAVVVLDTTGRISW
                     KTAKARLWLSEYLEFDDDAARLPPELDAWAAECMARGECGEYGIPPRGRGLRELRLRL
                     TPSLKAGEYALLLDERVCEPSSETALGAAYQLTAREGEVLLWLAKGKTNRDIGDILGM
                     SPRTVNKHLEHIYVKLGVETRAAATALVLTHLSKARAN"
     gene            3670..4707
                     /locus_tag="VGC69_00945"
     CDS             3670..4707
                     /locus_tag="VGC69_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic amidase"
                     /protein_id="HEY0293878.1"
                     /translation="MRHGDISSSNDSVGVAVVNYKMPRLHTRAEVLANARRIADMVVG
                     MKTGLPGMDLVIFPEYSTHGIMYDPEEMYETASTVPGEETAIFAQACRQAKVWGVFSL
                     TGEQHEEHPNKAPYNTLILMNDKGEIVQKYRKIMPWVPIEGWYPGDRTYMTEGPKGLK
                     ISLIICDDGNYPEIWRDCAMKGAELVVRCQGYMYPAKDQQVLVAKAMAWMNNIYVAVA
                     NAAGFDGVYSYFGHSAIVGFDGRTLGECGEEEMGIQYAELSVGLIRDARKNMQSQNHL
                     FKLLHRGYTGKINSGEAPAGEAACPYSFYTDWINDPEGTRKRVESFTRSTVGTPECPI
                     EGIPNQPDAHR"
     gene            complement(4762..5187)
                     /locus_tag="VGC69_00950"
     CDS             complement(4762..5187)
                     /locus_tag="VGC69_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY0293879.1"
                     /translation="MFNPSREQVRAFFIETWRKHRANEVLTPLESMALDWMLEHPEYY
                     ADLENPEAGLAEYSVEEGRTNPFLHLSMHLAIAEQLSIDHPRGIRAAYQRLVGRSDAH
                     HAAHEIMECLGRVVWEAQRLGTPLDSDTYIDLIRQRAER"
     gene            5347..6600
                     /gene="icd"
                     /locus_tag="VGC69_00955"
     CDS             5347..6600
                     /gene="icd"
                     /locus_tag="VGC69_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0293880.1"
                     /translation="MSYQHIKVPEGGQKITVNADLSLNVPDQVVIPYIQGDGVGEDIS
                     PVMRAVVDAAVEKAYGGQRRIHWMEVYAGQKATQVYGAEAWLPEETVDAIREYAVAIK
                     GPLNTLAGGAIRSLSVALRQRLDLYVCLRPVRYIKGVPSPVRNPEKTDIVVFRENSED
                     IYAGIEYAAESDEAKRLIQFLQAELGATLRFPGTSAIGIKPVSREGSERLVRQAIQYA
                     IDHERSSVTLVHKGNIMKFTEGGFRDWGYALARNEFGARVIDNGPWCRIKNPRTGRDI
                     IVKDVLTDAFMQRVLLRPAEFDVIATLNLNGDYISDAVAAQVGGHGIAPSANMSASVA
                     IFEVTHGTAMKYAGKDYVNPGSQILAAEMMLRRMGWLEPADLIIASMEKNIVAKKVTY
                     DFARLIPGAEQVSCSGFGRAMIANM"
     gene            complement(6724..7512)
                     /locus_tag="VGC69_00960"
     CDS             complement(6724..7512)
                     /locus_tag="VGC69_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY0293881.1"
                     /translation="MLSPLYSRAARRCALPAAGVLLAALACPALADEAPSGDTPSYTL
                     TANLTLASEYRYRGLMQTAGNPAIQGGFDFSHASGFYLGNWNSNISWLSDGNGKVSAP
                     IEMDFYGGYKGKITDDVPFDLGVLRYQYPGDYPSGVVSPDTTELYAGTGYGPVTFKYS
                     YAPTNLFGFDHSQHSQYADLTANFDTGVWGLTVNTHVGYQYVRNVDHGSYWDWKLGLT
                     KDFGQGWSASVAYIDTNADRTVYTNPQGRYIGRATGLASVTKTF"
     gene            complement(7658..8347)
                     /gene="kdpE"
                     /locus_tag="VGC69_00965"
     CDS             complement(7658..8347)
                     /gene="kdpE"
                     /locus_tag="VGC69_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HEY0293882.1"
                     /translation="MFDYRPTVLLIEDDAHIRRFVRESLAAEGCEVFEADTVQRGLIE
                     AGTRQPDAVVLDLGLPDADGMTLIRELRGWTDVPVLVLSARSHETDKIAALDAGADDY
                     LSKPFGVGELLARLRVLLRRHARDAANAADVMFGDVHVDLARRTVTRAGQAVHLTQIE
                     YRLLAALLAQRGKVLTHRELLREVWGPSHVESSHYLRIYMGHLRQKLEADPAQPAYLL
                     TEIGVGYRFAA"
     gene            complement(8360..11101)
                     /locus_tag="VGC69_00970"
     CDS             complement(8360..11101)
                     /locus_tag="VGC69_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEY0293883.1"
                     /translation="MTDPGNPRPDPDQLLKHLDNAAADARRGRLRIYFGASAGVGKTY
                     AMLAAARAQRDQGVDVVAGVIETHGRRETAALLDGLPQLPPQAIAYRDRTLEEFDLPG
                     ALARRPQLLLVDELAHTNVPGARHAKRWQDVDELIGAGIDVWTTLNVQHLDSLNEAVG
                     GITGIKIWETVPDAVFDAADEIILVDLSADELLRRLQEGRVYLPEQARHARRNFFRKG
                     NLIALRELALRRTADHVDDDVRAYRHERAIEPVWRTRETVLACLVPDPQAGQVVRSAH
                     RLARQLECDLHVITVATPRIAALPQARRHLLEQAMALAEQLGARTETLAGPDPVQAAA
                     RYIRRHNISKAVVGRGDARGRPAAWLDALLGAAWPRRHGSLAESLARACPDVDVVRVA
                     TPQAQHEAAAPGAATGDEIGDTPPRPLGGYLWAAVYCVAATLVSSAAFPLLHQTNIVM
                     FYLLAVVFAALRHGRGPAALAAAVSVGLFDFFFVQPFWSFAVSDVQYLLTFAVLLGVG
                     LLVGQLTAGLRAQAQTAAKREADTRGLYEFARELSSALQAEQIVGAADSFLKAAFDAR
                     SALYVQGLDDRLHRAPTTADMTEPDAALAQWAFDHGQAAGTGTATLSNSPMLYLPLRA
                     PMRVRGVLALEAPRRALYAEPETRRQLDAYATLIAIALERLHYVEVAQQALVGIESER
                     LRNALLAAVSHDLRTPLTGLIGMSDTLARNAAALPDSTRELAEAIRDQARRMHALVVN
                     LLDMARLQSHEAPLRREWQSIEELVGAALAAMKESLDGRPVTVARLSDLPLVECDGVL
                     IERVLCNLLENAAKYTPPGSPLRIGAALQGGEMQVAVSDRGPGVPPGEHQRIFEKFTR
                     GAHESALPGVGLGLAVCRAILEAHRGRIWVDPDYAEGARFVFALPLGVPPTLDAEPQ"
     gene            complement(11176..11781)
                     /gene="kdpC"
                     /locus_tag="VGC69_00975"
     CDS             complement(11176..11781)
                     /gene="kdpC"
                     /locus_tag="VGC69_00975"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEY0293884.1"
                     /translation="MQASRANPGLLRPALALFIALSAITGLAYPLATTGVARLLFPHQ
                     AAGSLIERDGKVVGSSLIGQAFSSPGYFWSRPSATAPMPYNAAASSGSNLGPTNPGLT
                     QAVKDRIAALDAADPGNTAPVPVDLVTASASGLDPHISPAAADYQAARVARARKLPEA
                     DVRRLVRAHTEAGPWLGLLGDPTVNVLELNLALDQLAGAGH"
     gene            complement(11828..13984)
                     /gene="kdpB"
                     /locus_tag="VGC69_00980"
     CDS             complement(11828..13984)
                     /gene="kdpB"
                     /locus_tag="VGC69_00980"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY0293885.1"
                     /translation="MDTRRLSPATAAPAAAAPRSFGFWSRSLMVPALRDSLVKLAPAT
                     QWRNPVMFVVYVGSILTTVLWLMALAGHAEAPAGFILAVSAWLWFTVLFANFAEALAE
                     GRGKQQAATLRGLRTTIQARVLNLANGKAPSDQALRDATPEAWRDRTALKPSGELRKG
                     DIVLVLAGDTVPGDGQVVAGIASVDESAITGESAPVIRESGGDFSSVTGGTRVLSDWI
                     FVRIAANPGESFLDRMIAMVEGAKRQKTPNELALTILLVGLTIVFLLVCVTLLPFSAY
                     AVDAAGGGGVVSVTALVALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEA
                     AGDVDVLLLDKTGTITFGNRQASAFLPAPGVSERELAEAARLASLADETPEGRSIVAL
                     ADRQLGAPAARLPQAEFVPFTAQTRMSGVDLDGRAIRKGAVDAVAAWLRDMGAAALPA
                     PMSRDADTVARRGSTPLAVADGARALGVIELKDIVKPEIAPRFAELRRMGIKTVMITG
                     DNKLTAAAIAAEAGVDDFLAEATPEAKLKLIRTYQQEGRLVAMTGDGTNDAPALAQAD
                     VAVAMNSGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYF
                     AIIPAAFAAVYPQLGVLNIMHLATPASSILSAVIFNALIIIALIPLALKGVRYRPLGA
                     AILLRRNLLIYGLGGLLVPFAGIKVIDMMLAALGWA"
     gene            complement(14082..15860)
                     /gene="kdpA"
                     /locus_tag="VGC69_00985"
     CDS             complement(14082..15860)
                     /gene="kdpA"
                     /locus_tag="VGC69_00985"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY0293886.1"
                     /translation="MDTRFLALLAVFLVILLAIAPFLGRYIREAMENGRYRLTGWGRP
                     VERVLYRAAGVDPQAEMGWKRYALAGLLFSVLGVVAVYALQRLQNLLPLNPADMGAVS
                     PDSSFNTAVSFVTNTNWQGYAGESTMSYLTQMLALTVQNFFSAALGIAVLFALIRGLT
                     RHSARAIGNFWVDMVRATLYVLLPLSFVLALVFVGQGAIQNFAPYAQAQTIETLNYDA
                     PKLDAQGQPVLDAQGKPVAESQATQTQSIAMGPVASQEAIKMLGTNGGGFFNANSAHP
                     YENPTPLANLLQMLAIFAIPAALCFSFGEMAGSRRQGVVILATMTLMFIVFALVTAHY
                     EYQPSPALLHAGIAPDAGNMEGKEARFGIGPTALFATITTAASCGAVNGMHDSFTAMG
                     GLSPMLLIQLGEIIFGGVGSGLYGMLAFAILAVFIAGLMIGRTPEYLGKKIDAFDMKM
                     VSIVLLATPFLVLAGTAVAVLADAGRAGILNPGIHGYSEILYAFSSAANNNGSAFAGL
                     SANTPFYNILLGIAVWLGRYLPIVAILGLAGSLAAKPRLAAGPGTMPTTGPLFVVLLI
                     GSILLVGALTYVPALALGPVAEHLQH"
     gene            complement(15860..15949)
                     /gene="kdpF"
                     /locus_tag="VGC69_00990"
     CDS             complement(15860..15949)
                     /gene="kdpF"
                     /locus_tag="VGC69_00990"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEY0293887.1"
                     /translation="MTFIYGLSGLLAALLLAYLLIALFKPEKF"
     gene            16684..16950
                     /locus_tag="VGC69_00995"
     CDS             16684..16950
                     /locus_tag="VGC69_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/substrate symporter small subunit"
                     /protein_id="HEY0293888.1"
                     /translation="MSLPAPPVPPAAARAYWRRNLRYIALLLAVWLLLTLAPALFTGW
                     LSFDFIGWPFPFWLAAYGAPLAYLFIVAVYAWLMSRADRPGRDR"
     gene            16950..19013
                     /locus_tag="VGC69_01000"
     CDS             16950..19013
                     /locus_tag="VGC69_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEY0293889.1"
                     /translation="MQSARGPREFSLWLRRIYLLYTAGFCLLVGLLALAEVAGLARNW
                     IGYVFLIITVSLYASIGILCRTSDQVEYYVAGRRVPAFYNGMATAADWMSVASFIGVA
                     GTLYLTGYGGLAYVMGWTGGYVLVALLLAPYLRKFGQYTIPDFLGARFGGNVPRLAGV
                     ACAVLCSFTYLVAQIYGVGIITTRLTGIPFELGIFVALGGMLVCSFLGGMRAVTWTQV
                     GQYIILVIAYLVPVVWLSVKDTGSPIPQLSAGSVLRQVTEKELDLRNDPAEQAVRRLW
                     RAKAQEMTQRLEALPQSWAQEKERLRTRLSERIAADAPMVEVRTLERELADFPADPES
                     ARAAWSQARSVYEARGSPPVPHAQSFPGGDAAEKRNMRVNFLALVLCLMLGTAGMPHI
                     LTRSYTTPSVGDARRSVFWALLFILLLYFTAPALALLVKYEVYTQLVGSSYASLPAWV
                     HAWGTVDPNLMNVIDINHDGIVQLGEIGIGADVVVLAMPEIGGLPYVISGLVAAGALA
                     AALSTADGLLLTLSNSLSHDMLYRVVSPRMPAGRRVIASKVLLLLVAFAAAWVAARKP
                     ADILIMVSAAFSFAASSFFPVLVMGMFWKRANKWGATLGMAVGPVVTFAYMAYTDPWL
                     REWVFGVPRTDPVSLWWGIQPICAGLFGAPAAFLTIVAVSLLTRRPDTATEALVDYLR
                     EPAES"
     gene            complement(19022..19690)
                     /locus_tag="VGC69_01005"
     CDS             complement(19022..19690)
                     /locus_tag="VGC69_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0293890.1"
                     /translation="MRSKSKGGLADLKKLHQEFEQTRQERERAEREAAAARARREQAA
                     PAHDVDAFRKAMQGAAPIKAHNLVERRPAALPVDPVVAARRAHALGEKPGRADAGISD
                     GGVAHLLSENGTAYVRADAAPDTARKLKQGHWRAGAELDLHGLRVEQARHAVISFLDE
                     CLSQGIRCVRIVHGKGYGSEGMSPVLKDKARAWLAQKTDVLAFSEASPREGGSGALLV
                     LMQV"
     gene            complement(19862..>20733)
                     /gene="trxB"
                     /locus_tag="VGC69_01010"
     CDS             complement(19862..>20733)
                     /gene="trxB"
                     /locus_tag="VGC69_01010"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY0293891.1"
                     /translation="ANLKPMLITGLAQGGQLMTTTEVDNWPADVDGVQGPDLMERFQK
                     HAERFETEMVFDHIDKVDLSKRPFTLTGDTGNLYTCDALIIATGASAKYLGLESESAF
                     MGRGVSACATCDGFFYRNKDVVVVGGGNTAVEEALYLSNLCRKVTVIHRRDKFRAEPI
                     LIDRMMDKVANGNMALKLHSVLDEVLGDDGGVTGARIRNLQTGATEDVDVMGCFIAIG
                     HQPNTGLFEGQLKMKDGYIVTQSGHNGMATLTSVPGVFAAGDVQDYVYRQAITSAATG
                     CMAALDAQHWLEG"
BASE COUNT         3296 a         6642 c         7388 g         3407 t
ORIGIN      
        1 cctgccgaaa aagaccgccg ccaaccgcgt ccgctggtgg acggcgatgc tgtgcgccgg
       61 gccgggctgg atcgtgccgg gggcggccaa gatgctggcc ggcgccttcc tggcctatct
      121 ggcgctgagc cacggcgtgc cgggcgccaa ggcggccgat cccacgcaga tgtaccgggt
      181 ggctttcggc tacgtgctgt cgaaccccgc ctgggtcctg gcgctgacca cgctgttcgt
      241 ggtggtgtcg caggtcaaga tcaacgtcac caatgcctat gccggctcgc tggcctggtc
      301 gaacttcttc gcccgggtga cgcacagcca tccgggccgc gtggtgtggc tggtgttcaa
      361 tgtgctgatc gccatctgcc tgatggaaat gggcgtgttc gacgcgctcg agcatgtgct
      421 gaccgtgttc gcgcacgtgg ccctggcctg gatcggcgcg ctggtggccg atctggtggt
      481 caacaagccg ctgggcctga gccccaaggg catcgagttc aggcgcgcct acctgtacga
      541 catcaacccg gtgggggtgg gcgcctcgct gttctcgctg gtcgtgtcgc tggtcgccga
      601 cctcggcggg ttcggccaga cggcgcacgc gctgtcgccc ttcatcgcgc tgctggcggc
      661 cttcgcggcg gcgccggtca tcgcgcgcgc gaccggcggg cgctactacc tggcgcgccg
      721 gcccgcctcg ctgacgacgc gccagggcga ccatggcctg acctgcgtga tctgcgccaa
      781 caccttcgag accggcgaca tggcgcattg cccggcctac aacggcgcga tctgttcctt
      841 gtgctgcacg ctggacgcgc gctgccacga ccgctgcaag accaattcgc gcctgcagga
      901 ccagataggc gaggccctgc gccgcctgct gcccaggcac ctggccacgc cgctgttcca
      961 caaccggctg gcgcattacc tgctggtgct cggcaccatg gcgggcgggc tggcggccat
     1021 cctctcgctg atctactacc aggaaaccgt cgcctacgcg tcgatcggcg ccgatatcgg
     1081 cgtgctcacc gatacgttca cgaagctgtt cgccgcgctg gtcctgatcg gcggggtcgt
     1141 ggcttggtgg atggtgctga ccaacgaaag ccggcaggtg gcgcaggagg agtccgaccg
     1201 ccagaccaac ctgctcctgc acgaaatcga ggcccaccgc cgcaccgacg cccagctgca
     1261 gcaggccaag gaggtcgccg aggccgccaa cctggccaag agccgcttca tgggcggcat
     1321 gagccacgaa ttgcgcgcgc cgctcaacag catcctgggc tatgcgcaga tcttgcagcg
     1381 cgaccccaca ttgccgtcgg cccggcgcga ggccatcgac gtcatccatc gcagcggcaa
     1441 gcatctggta agcctgatcg acggcctgct cgacatcgcc cgcatcgagg ccggcaagct
     1501 gcgtctcgaa acgaccgaac tgcggctgcc cgatttcctg gaccagatcg tcaagatgtt
     1561 ccatccccag accgcgctca aggggctgcg cttcgactac ctcgtttcgt ccgacctgcc
     1621 gcgcatcgtg cacatcgacg agaagcggct gcgccagatt ctgatcaacc tgatgagcaa
     1681 cgccgtcaaa ttcacgcgcg agggcggcgt ggcgctgcgg gtgggctacg cctccgacat
     1741 ggtgacgatg gtggtcgagg acagcggcgc cggcatcccg gccgaagact tcgagcgtat
     1801 tttcatgcct ttcgagcgga gctgggccgc catcggccag gccgacaccg gcacgggcct
     1861 gggcctgacc atctgccgca tgctgaccaa catcatgggc ggcgagctca cggtcaagag
     1921 cgaagtcgga cggggcagca tcttcacgct caagctcttc ctgcccgagg tgcgctcgtc
     1981 gagcggcagc ctgcgcccgg ccggcctggt gacgggctat gcggggccgc ggcgcggcgt
     2041 gctggtggtc gacgaccagc cttcgcagcg gcgcgtcctg cgcgacatgc tggagcccct
     2101 ggggttcgtg gtgcacgagg cgccggacgg ccacgcctgc ctgctgcgcg cggccgacct
     2161 gcggcccgac ctgatcctga tggatatttc catgccgaat atgccgggcg acgaggccgc
     2221 gcgcatgctg cgccagcagg gcgtggccac gccggtgatc atcgtctcgg ccaatgtcta
     2281 cagcctgggc ccgcacgacg ccacgacggc gagcgactgg ctgttcaagc cggtggtgct
     2341 ggacgagctg ctggccaaga tcggcgcgca tctcaagttg caatggatcg tccagcccga
     2401 ggccgaagag agcggcccgg cggacctgag cgggatcacc ctggcgcggg aagacgccga
     2461 gtccctcctg gagctgggcg cgatgggcta catcaagggc atcatggcgc gcctggacga
     2521 tatccgcgac cgcgcgccga aagccgcgcc gctggtcgat cacctggcgt cgctggcaaa
     2581 ggaattccgt ttgaccgaat acaactctct cctgaaagat catgtacgac gccatttcac
     2641 gaccgactga aggcaaactc gtcatgctgg tcgacgacgc gccggacaac ctcaagatgt
     2701 tgtccgatgc gctcgaccag gcgggctata tggtgctggt cgccaccgac ggcgcctcgg
     2761 ccatcgagcg catggatttc gtgctgccgg acatcgtgct gatggacgcc atcatgcctg
     2821 gcctcgacgg cttcgaaacc tgccgccggc tcaagctgca ccgcagcgcc gcgcacgtgc
     2881 ccgtggtctt catgaccggc ctgaccgagc ccgagaacgt ggtgcgcggc ttccaggcgg
     2941 gcggcatcga ctacgtcacc aagcccatcg acgccgacgt ggtgctggcc cggctcgatg
     3001 cgcacctgcg cacggcgcgg atgatgtcgg ccgccatgaa tgccatggat gccacggcca
     3061 acgcggtggt ggtgctggac acgacgggcc gcatttcctg gaagaccgcc aaggcgcggt
     3121 tgtggctgtc ggaatacctg gagttcgacg acgacgcggc gcgcttgccg cccgagctgg
     3181 atgcctgggc ggccgagtgc atggcgcggg gcgagtgcgg cgagtatggc atcccgccgc
     3241 gggggcgcgg cttgcgcgaa ctgcggctgc ggctgactcc ttcgctcaag gcgggcgagt
     3301 acgcgctgct gctcgacgag cgggtgtgcg agccctcgtc cgaaaccgcg ctgggcgcgg
     3361 cctaccagct cacggcgcgc gagggcgagg tgctgctgtg gctggccaag ggcaagacca
     3421 accgcgatat cggcgacatc ctcggcatga gcccgcgcac ggtcaacaag cacctggagc
     3481 acatctatgt gaagctgggc gtggagacgc gcgccgccgc gacggcgctg gtattgacgc
     3541 acctgagcaa ggcgcgggcg aactgatccc ggcggcgtgg gggaaaaacg cggcggccct
     3601 acgttattcg acgtatggcc cggcggttcc gggatgtcca gaatggtctc ctacgcaagg
     3661 agccggatca tgaggcatgg cgatatctcc agcagcaacg acagcgtcgg cgtggccgtc
     3721 gtcaactaca agatgccgcg cctgcacacg cgcgccgagg tgctggccaa cgcgcgccgc
     3781 atcgccgaca tggtggtggg catgaagacg ggcctgccgg gcatggacct ggtgatcttc
     3841 cccgagtact ccacgcacgg catcatgtac gaccccgagg aaatgtacga gacggccagc
     3901 accgtgccgg gcgaggaaac cgcgatcttc gcccaggcct gccgccaggc caaggtgtgg
     3961 ggcgtgttct cgctcaccgg cgagcagcac gaggagcacc cgaacaaggc gccctacaac
     4021 acgctcatcc tcatgaacga caagggcgag atcgtgcaga agtaccgcaa gatcatgccc
     4081 tgggtgccca tcgagggctg gtaccccggc gaccgcacct acatgacgga aggccccaag
     4141 ggcctgaaga tcagcctcat catctgcgat gacggcaact accccgagat ctggcgcgac
     4201 tgcgccatga agggcgccga gctggtggtg cgctgccagg gctatatgta tccggcgaag
     4261 gaccagcagg tgctggtggc caaggccatg gcatggatga acaatattta tgtcgccgtg
     4321 gccaacgccg ccggcttcga tggcgtgtac tcctacttcg gccattccgc catcgtgggc
     4381 ttcgacggcc gtaccctggg cgaatgcggc gaggaagaaa tgggcatcca gtatgccgag
     4441 ctgtcggtcg gcctgatccg cgacgcgcgc aagaacatgc agtcgcagaa ccacctgttc
     4501 aagctcctgc accgcggcta taccggcaag atcaattccg gcgaggcccc cgccggcgag
     4561 gccgcctgcc cctacagctt ctatacggac tggatcaacg atcccgaggg cacccgcaag
     4621 cgggtggagt cgttcacgcg ttccaccgtg ggaacgcccg agtgcccgat cgaaggcatt
     4681 cccaaccagc cggacgcgca ccgctagccg gcatccgggg cggggcgcct gccggggcgg
     4741 ccgggcggcc cggccggggc ctcagcgctc ggcgcgctgg cggatcaggt cgatataggt
     4801 gtcgctgtcg agcggggtgc ccaggcgctg ggcttcccag accacgcggc ccaggcattc
     4861 catgatttcg tgtgcggcat ggtgagcgtc gctgcggccc accagccttt ggtaggcggc
     4921 gcggatgccg cgcgggtggt cgatggacag ctgctcggcg atggccaggt gcatcgacag
     4981 gtgcaaaaag gggttggtgc ggccttcttc gaccgagtac tcggccaggc cggcctcggg
     5041 gttttcgagg tcggcatagt attcggggtg ttcgagcatc cagtccagcg ccatggattc
     5101 cagcggcgtg agcacttcgt tggcgcgatg cttgcgccag gtttcgatga agaatgcgcg
     5161 aacttgctcg cgggagggat tgaacatggg gaaaaaggcc aatgcggcgg tgcagcgttt
     5221 attttacgcc tgcgggtgag ggaggttttt cctgccggac ggccgaggca agatagaatg
     5281 gtgccgaccc tatcttgacc gtacctggcc gggcagcaga cccgggccga cgcctgcgga
     5341 gcacccatgt cgtaccagca catcaaggtc cctgaagggg gccagaaaat caccgtcaat
     5401 gccgatctgt cgctgaacgt gccggatcag gtcgtgattc cctacatcca aggcgatggc
     5461 gtgggcgagg atatcagccc cgtgatgcgc gcggtggtcg atgccgccgt cgaaaaagcc
     5521 tacggcggac agcggcgcat ccactggatg gaagtctatg ccggccagaa ggccacgcag
     5581 gtctacggcg ccgaggcctg gctgcccgag gaaaccgtgg acgcgatccg cgagtacgcc
     5641 gtggcgatca agggccccct gaacaccctg gcgggcggcg ccatccgctc gctctccgtg
     5701 gcgttgcgcc agcgcctgga cctctacgtc tgcctgcgcc cggtgcgcta catcaagggc
     5761 gtgccgtcgc cggtgcgaaa ccccgaaaaa accgacatcg tggtatttcg cgagaactcc
     5821 gaggacatct acgccggcat cgaatacgcg gccgagtccg acgaggcgaa acggctcatc
     5881 cagttcctgc aggccgagct gggcgcgacg ctgcgctttc ccggcacctc ggccatcggc
     5941 atcaagcccg tgtcgcgcga aggatcggag cgcctggtcc gccaggccat ccagtacgcc
     6001 atcgaccatg agcgcagctc ggtcacgctg gtgcacaagg gcaacatcat gaagttcacc
     6061 gaggggggct ttcgcgactg gggctatgcc ctggcgcgca acgaattcgg cgcgcgcgtg
     6121 atcgacaatg gcccctggtg ccgcatcaag aacccccgca cgggccgcga catcatcgtc
     6181 aaggacgtgc tgaccgacgc cttcatgcag cgcgtgctgc tgcgcccggc cgagttcgac
     6241 gtgatcgcca cgctcaacct caacggcgac tacatctccg atgccgtggc ggcccaggtg
     6301 ggcggccacg gcatcgcgcc cagcgccaat atgtcggctt cggtcgccat cttcgaggtc
     6361 acgcatggca cggcgatgaa gtacgcgggc aaggactacg tcaacccggg ctcgcagatc
     6421 ctcgcggccg agatgatgct gcgccgcatg gggtggctcg aaccggcgga cctgatcatc
     6481 gccagcatgg aaaagaacat cgtggccaag aaggtcacct acgacttcgc gcgcctgatc
     6541 cccggcgccg agcaggtgtc gtgctcgggc ttcggccggg cgatgatcgc caatatgtaa
     6601 gtccggccgc cccggtcccg agaagatcga cgggcgcggg ctcgggcttc aggggcggca
     6661 gcggggcggc ccaggtcggg ggcgtgggct tggtcggaag aggacgggcc atgatggatg
     6721 tccttagaaa gtcttggtta cggatgccag accggtggcg cggccgatgt agcggccttg
     6781 ggggttggtg tagacggtgc ggtcggcgtt ggtgtcgata taggccaccg aggcgctcca
     6841 gccctggccg aagtctttgg tcagacccag cttccagtcc cagtacgaac cgtggtcgac
     6901 attgcgcaca tactggtagc ccacgtgggt gttcacggtc aggccccaga cgccggtgtc
     6961 gaaattggcc gtcaggtcgg cgtactggct gtgctggctg tggtcgaagc cgaacaggtt
     7021 ggtgggcgcg tacgagtact tgaaggtcac cgggccgtag ccggtgccgg catagagttc
     7081 ggtggtgtcg ggactgacca cgccggacgg gtaatcgccc gggtactggt agcgcaggac
     7141 gcccaggtcg aagggcacgt cgtcggtgat cttgcccttg tagccgccgt agaaatccat
     7201 ctcgatgggc gccgacacct tgccattgcc gtcggaaagc cagctgatgt tggaattcca
     7261 gttgcccagg tagaagccgc tcgcgtgcga gaagtcgaag ccgccctgga tggccgggtt
     7321 gccggccgtc tgcatgaggc cgcggtagcg gtattcgctg gccagggtga ggttggcggt
     7381 gagcgtgtag gaaggcgtgt cgccggaagg cgcctcatcg gccagggcgg ggcaggccag
     7441 cgcggcgagc aggacgcccg cggcaggaag ggcgcagcgc cgtgcggcgc gggaatacag
     7501 cggggacagc atgtcggttt tctcctgttg cgtacgccgg gaggcgcatg ccgcaacagt
     7561 agaaaaacgc gcataagccg cgcgtaaaga tcaggacgcc cggcgtaaat gccggataaa
     7621 tttttcaccg ccgagccggc gggtcggaat gcagtcttca ggccgcgaag cggtagccca
     7681 cgccgatctc ggtcagcagg taggcgggct gggcggggtc ggcttccagc ttctggcgca
     7741 agtggcccat gtagatgcgc aggtagtggc tgctttccac atgggaaggc ccccacacct
     7801 cgcgcagcag ttcgcggtgg gtcagcacct tgccgcgctg cgccagcagg gcggcgagca
     7861 ggcggtattc gatctgggtc aggtgcaccg cctggccggc ccgcgtgacg gtgcgccggg
     7921 ccaggtcgac gtggacgtcg ccgaacatca cgtcggcggc attggcggcg tcgcgcgcgt
     7981 ggcggcgcag cagcacgcgc agccgcgcca gcagctcgcc cacgccgaac ggcttgctca
     8041 ggtagtcgtc ggcgccggcg tccagggcgg cgatcttgtc ggtttcgtgg ctgcgcgcgg
     8101 acagcaccag caccggcacg tcggtccagc cgcgcagttc gcggatcagc gtcatgccgt
     8161 cggcatcggg caggcccagg tcgagcacca cggcgtcggg ctggcgcgtg ccggcttcga
     8221 tcaggccgcg ctggacggtg tcggcctcga agacttcgca gccctccgcg gcgagcgact
     8281 cgcgcacgaa gcgccggatg tgggcgtcgt cttcgatcag cagcacggtg ggtcggtagt
     8341 cgaacatggc gatcggggct tattgcggtt cggcgtcgag ggtgggcggc acgcccagcg
     8401 gcagggcgaa cacgaagcgc gcgccttcgg cgtagtccgg gtcgacccag atgcggccgc
     8461 ggtgcgcctc caggatagcc cggcagacgg ccaggcccag gccgacgccg ggcagggcgg
     8521 actcatgcgc gccccgcgtg aatttctcga aaatgcgctg atgctccccg ggcggcacgc
     8581 ccgggccgcg atcgctgacg gcgacctgca tttcgccgcc ttgcagggcc gcgccgatgc
     8641 gcagggggct gccgggcggg gtgtatttgg cggcattttc gagcagattg cacagcacgc
     8701 gttcgatgag cacgccgtcg cattccacca ggggcaggtc ggacagccgc gccacggtga
     8761 cgggccggcc gtccaggctt tccttcatgg cggccagtgc cgcgccgacc agttcttcga
     8821 tggattgcca ttcgcgccgc agcggcgctt cgtggctctg cagcctggcc atgtccagca
     8881 ggttgacgac cagggcgtgc atgcggcggg cctggtcgcg tatggcttcg gccagttccc
     8941 gcgtggaatc cggcagggcg gcggcgttgc gggccagggt gtcggacatg ccgatcaggc
     9001 cggtcagggg cgtgcgcagg tcgtgcgaca cggccgccag cagcgcgttg cgcaagcgct
     9061 cggactcgat gccgaccagg gcctgctggg ccacttcgac gtagtgcagg cgttccagcg
     9121 caatggcgat cagcgtggcg taggcatcga gctggcgccg ggtttccggt tcggcgtaga
     9181 gcgcgcggcg cggcgcctcc agggccagga cgccgcgcac gcgcatgggg gcccgcagcg
     9241 gcaggtacag catcgggctg ttgcttagcg tggctgtgcc ggtgccggcg gcctgcccat
     9301 ggtcgaaggc ccattgcgcc agcgccgcat cgggttccgt catgtcggct gtcgtgggcg
     9361 cccggtgcag ccggtcgtcc aggccctgca cgtacagggc gctgcgcgcg tcgaaggccg
     9421 ccttcaggaa gctgtccgcc gcgccgacga tttgttcggc ctgcagggcc gatgaaagtt
     9481 cgcgcgcgaa ctcgtacagg ccccgggtat cggcttcgcg cttggccgcg gtttgcgcct
     9541 gggcgcgcag tccggccgtc aactggccga ccagcaggcc cacgcccagc agcacggcga
     9601 atgtcagcag gtactgcacg tcggacaccg cgaaagacca gaacggctgc acgaagaaaa
     9661 agtcgaacag gcccacgctg accgcggcgg ccagcgccgc ggggccgcgg ccgtgacgca
     9721 gggcggcgaa caccaccgcc agcaggtaga acatgacgat attggtctgg tgcagcagcg
     9781 ggaaagcggc gctcgatact agggtggccg ccacgcaata aacggcggcc cagagatagc
     9841 cgccgagcgg acggggcggg gtgtcgccaa tctcatcgcc ggtcgcggcg ccgggcgcgg
     9901 ctgcctcgtg ctgcgcctgc ggtgtggcca cgcggacgac atcgacgtcg gggcaggccc
     9961 gcgccaggga ttcggccagg ctgccgtggc gccgcggcca ggcggcgccc agcagcgcat
    10021 cgagccaggc ggccggccgg ccgcgggcat cgccgcggcc gaccacggcc ttgctgatgt
    10081 tgtgccggcg tatgtagcgg gcggccgcct ggacggggtc ggggccggcc agggtctcgg
    10141 tgcgcgcgcc cagttgttcg gccagggcca tggcctgctc cagcaggtgc cgccgcgcct
    10201 gcggcagcgc ggcgatgcgc ggcgtggcga ccgtgatcac gtgcaggtcg cattcgagct
    10261 ggcgggccag gcggtgggcg ctgcgcacca cctggcccgc ctgcgggtcg gggacgaggc
    10321 aggccagcac ggtctcgcgc gtgcgccaca cgggctcgat ggcgcgttca tggcggtagg
    10381 cccgcacgtc gtcgtcgacg tggtcggcgg tgcggcgcaa ggccagttcg cgcagcgcga
    10441 tgaggttgcc cttgcggaag aaattgcgcc tggcatggcg cgcctgctcg ggcaggtaga
    10501 cccggccttc ctgcaggcgg cgcagcagtt cgtcggccga caggtccacc aggatgatct
    10561 cgtcggccgc gtcgaacacg gcatcgggca cggtttccca gatcttgatg ccggtgatgc
    10621 ctccgacggc ttcgttgagg ctgtcgaggt gctgcacgtt gagcgtggtc cagacgtcga
    10681 tgccggcgcc gatcagctcg tcgacatcct gccagcgctt ggcgtgccgc gcgccgggca
    10741 cattggtgtg cgcgagctcg tcgaccagca gcagttgcgg ccggcgcgcc agcgcgccgg
    10801 gcaggtcgaa ttcttccagg gtgcggtcgc ggtaggcgat ggcctgaggc ggtagctgcg
    10861 gcaggccatc gagcagtgcg gcggtttcgc gccggccatg cgtttcgatg acgccggcga
    10921 ccacgtccac gccctgatcg cgttgcgcgc gggcggcggc cagcatcgca taggtcttgc
    10981 cgacccccgc ggaggcgccg aaatagatgc gcaggcggcc gcgccgtgca tcggccgccg
    11041 cgttatccag atgcttgagc agttggtcgg gatcgggtcg cggattgccg ggatcggtca
    11101 tgtaagcagt cgcctgcaga gacggtgcct actatacgcc cgatccgtcc cggtctttgc
    11161 gcggggcggc atggctcaat gccccgcgcc ggccagctgg tccaacgcca ggttcagttc
    11221 cagcacgttc accgtggggt cgccgagcag gcccagccag gggccggcct cggtgtgggc
    11281 ccgcaccagt ctgcgcacat cggcttcggg cagcttgcgc gcgcgggcta cccgggcggc
    11341 ctggtaatcg gccgccgccg gactgatgtg cggatccagg ccgctggccg aggccgtgac
    11401 caggtcgacc ggcaccgggg ccgtgttgcc cggatcggcg gcgtcaagcg cggcgatgcg
    11461 gtccttcacg gcctgggtca ggcccgggtt ggtcggcccc aggttcgagc cgctcgacgc
    11521 ggcggcgttg tagggcatgg gcgccgtggc cgatgggcgg ctccagaaat agcccggtga
    11581 actgaaggcc tggcctatga gcgacgaacc cacgaccttg ccgtcgcgct cgatgagcga
    11641 gccggcggcc tggtgcggaa agagcagcct ggcgacgccg gtggtggcca gcgggtaggc
    11701 caggccggta atcgccgaca gggcgatgaa gagcgccagg gccgggcgca gcaggccggg
    11761 gtttgcgcgg gaagcttgca tgatggattc cttggtgcgt gaaaagaggg aggcgtgcgc
    11821 tcaggcctta ggcccagccc agggcggcaa gcatcatgtc gattaccttg atgccggcga
    11881 agggcaccag cagcccgccc aggccgtaga tcagcaggtt gcgccgcagc aggatggccg
    11941 cgcccagggg gcggtagcgc acgcccttca gggccagggg gatcagcgcg atgatgatga
    12001 gcgcgttgaa gatcacggcc gacaggatgc ttgatgccgg cgtggccagg tgcatgatgt
    12061 tgagcacgcc cagctgcggg tacaccgcgg cgaaggcggc cgggatgatg gcgaagtact
    12121 tcgccacgtc gttggcgatg ctgaaggtgg tgagcgagcc gcgcgtcatc agcatctgct
    12181 tgccgatctc gacgatctcg atcagcttgg tggggttgga atccaggtcc accatattgc
    12241 cggcctcttt ggcggcctgg gtgcccgagt tcatggccac ggccacgtcg gcctgggcca
    12301 gcgcgggcgc gtcgttggtg ccgtcgcccg tcatggccac cagccggcct tcctgctggt
    12361 aggtgcggat cagcttgagc ttggcctcgg gcgtggcttc ggccaggaaa tcgtccaccc
    12421 cggcctcggc ggcgatcgcg gcggccgtga gcttgttgtc gccggtgatc atcaccgtct
    12481 tgatgcccat gcggcgcagc tcggcaaagc gcggggcgat ctccggcttg acgatgtcct
    12541 tgagctcgat cacgcccagg gcgcgcgcgc cgtcggccac ggccagcggc gtgctgccgc
    12601 gccgcgccac ggtgtccgca tcgcgcgaca tgggcgcggg cagcgcggcg gcgcccatgt
    12661 cgcgcagcca ggcggccacg gcatcgaccg cgcccttgcg gatggcgcgg ccgtccaggt
    12721 ccacgccgct catgcgggtc tgcgccgtga agggcacgaa ttcggcctgg ggcagtctgg
    12781 ccgccggggc gcccaattgc cggtcggcca aggccacgat gctgcggccc tcgggcgtct
    12841 cgtcggccag cgaggccagg cgcgcggcct cggccagttc gcgttcgctg acgccgggcg
    12901 cgggcaggaa ggccgaggcc tggcggttgc cgaaggtgat ggtgccggtc ttgtccagca
    12961 gcagcacgtc cacgtcgccg gcggcttcca ccgcgcggcc cgaggtggcg atgacattgg
    13021 cctgcatcat gcggctcatg ccggccacgc cgatagcaga gagcaggccg ccgatggtgg
    13081 tggggatgag gcacaccagc agggcgacca gtgccgtcac cgacaccacg ccgccaccgc
    13141 cggccgcgtc caccgcatag gccgagaagg gcagcagcgt gacgcagacc agcaggaaca
    13201 cgatggtcag gcccaccagc aggatggtca gcgccagctc gttgggcgtc ttctggcgct
    13261 tggcgccctc gaccatggcg atcatgcggt cgagaaagct ctcgccggga ttggctgcaa
    13321 tgcgcacgaa gatccagtcc gacagcacgc gggtgccgcc ggtcaccgac gagaagtcgc
    13381 cgcccgactc gcggatcacc ggcgccgatt cgccggtgat ggcgctttcg tcgaccgagg
    13441 cgatgccggc cacgacctgg ccgtcgccgg gcacggtatc gccggccagc accagcacga
    13501 tgtcgccctt gcgcagttcg cccgacggct tgagcgcggt gcggtcgcgc caggcctcgg
    13561 gcgtggcgtc gcgcagggcc tgatcgctgg gcgccttgcc gttggctaga ttcagcacgc
    13621 gcgcctggat ggtggtgcgc aggccgcgca gcgtggcggc ctgctgcttg ccgcggcctt
    13681 cggccagcgc ctcggcgaag ttggcgaaca gcacggtgaa ccacagccag gccgagacgg
    13741 ccaggatgaa gccggccggg gcttcggcat ggccggccag ggccatgagc cacagcacgg
    13801 tggtgaggat gctgccgacg tagacgacga acatcacggg gttgcgccat tgggtggccg
    13861 gcgccagctt gaccaggctg tcgcgcagcg ccggaaccat gagcgagcgc gaccagaagc
    13921 cgaacgagcg gggcgccgcg gcggcggggg cggccgtggc gggggaaaga cgacgagtgt
    13981 ccatgatcat tccagcgatg tcagctccgc cgggccgccc caaggggctg acagtcccct
    14041 cggggggcag cgaacggcgt gagcgtgagg gtcatatgat ttcaatgctg caggtgttcg
    14101 gcgacggggc ccagcgccag ggccggcaca taggtcaggg cgcctaccag caggatggag
    14161 ccgatcagca gcaccacgaa cagcggcccg gtggtcggca tggtgccggg gccggccgca
    14221 aggcgcggct tggcggccag cgagccggcc aggccgagga tggccacgat gggcaggtag
    14281 cggcccagcc agacggcgat gcccagcagg atgttgtaga agggcgtgtt ggccgacagg
    14341 ccggcgaagg cgctgccgtt gttgttggcc gccgacgaga aggcgtagag gatctccgag
    14401 tagccgtgta tgcccgggtt gagaatgccc gctcggccgg cgtcggccag cacggccacc
    14461 gcggtgccgg ccagcaccag gaagggcgtg gccagcagca cgatggacac catcttcatg
    14521 tcgaaggcgt cgatcttctt gcccaggtat tcgggcgtgc ggcctatcat cagcccggcg
    14581 atgaagacgg ccaggatggc gaaggccagc atgccgtaga gccccgagcc cacgccgccg
    14641 aagatgatct cgcccagctg gatcagcagc atcggcgata gcccgcccat ggccgtgaac
    14701 gagtcgtgca tgccgttgac cgcgccgcac gaagccgccg tggtgatggt ggcaaagagc
    14761 gcggtggggc cgatgccgaa gcgcgcctcc ttgccttcca tattgccggc atccggcgcg
    14821 atgccggcgt gcagcagggc cggggacggc tgatactcgt aatgcgcggt gaccagtgcg
    14881 aagacgatga acatcagggt catcgtggcc aggatcacca cgccctgcct gcggctgccg
    14941 gccatttccc cgaagctgaa gcacagcgcc gcgggtatgg cgaagatcgc cagcatctgc
    15001 agcaggttgg ccagcggcgt ggggttctcg tagggatgcg ccgagttggc gttgaagaag
    15061 cccccgccgt tggtgcccag catcttgatg gcctcctgcg aggcgacggg gcccatggcg
    15121 atgctctggg tctgggtggc ctggctttcc gcgacgggtt tgccctgcgc gtccagcacg
    15181 ggctggcctt gcgcgtcgag cttgggcgcg tcgtagttca gcgtctcgat ggtttgcgct
    15241 tgcgcgtagg gggcgaagtt ctggatggcg ccctggccca cgaacactaa cgccagcacg
    15301 aaggacaggg gcagcagcac gtacagcgtg gcgcgcacca tgtcgaccca gaagttgccg
    15361 atggcgcggg cgctatggcg ggtcaggccg cggatcaggg cgaacagcac ggcaatgccc
    15421 agcgctgccg aaaagaagtt ctgcacggtc agcgccagca tctgcgtgag atagctcatc
    15481 gtcgattcgc cggcgtagcc ctgccagttg gtgttggtca cgaacgagac ggcggtgttg
    15541 aatgacgaat cgggcgagac ggcgcccatg tcggccgggt tgagcggcag caggttctgc
    15601 aggcgctgca aggcatagac ggccacgacg cccagcacgc tgaacagcag cccggccagg
    15661 gcatagcgct tccagcccat ttcggcctgg gggtccacgc ccgcggcccg gtagaggacg
    15721 cgttcgacgg ggcggcccca gccggtcagg cggtatcggc cgttttccat ggcctcccgg
    15781 atataccggc ccaggaacgg ggcgatcgcc agcaggatca cgaggaacac cgccagcagt
    15841 gcgagaaagc gggtatccat cagaatttct ccggcttgaa caaggcgatc agcaaatacg
    15901 ccagcagcag ggcggcgagc aggccgctga gcccataaat gaaggtcatg gccgttctcc
    15961 ttgcgagagg gccgagcaga acaccgtcag gccccagacc aggccggcca gcgcgccgaa
    16021 aatggcgaaa tacagcaagt ccacgttttt ctcccccggg gcatgcgccc cttcgtgtgc
    16081 aggcccggcg gcattgaccg ccagggcctg cgcagattac ggagacgggg gtaaaaacgg
    16141 gataaagcct ggggcgggca gtataaaaag cgtgtaaaaa cccggctttc gggcgtgccg
    16201 cgcagcgtcc tgccagggcg ccgattgatt gcaccaattt ggtgctttat tgcgggcctc
    16261 aggaaaaccc tgtttctcga gacttgccag gattttgtcg aaccgatagg ctttgaaggg
    16321 gcctaagtcc taaatagggt ggttagagcc ctaaattgca ccaatgttgt gcaaatcatg
    16381 gcccagaaaa tgcatcagac tagggcattc ccctagtgtt ataagctcgt aactggtgca
    16441 caatttgttc catgcacggt gaggagacac aataagccca aagggcaaaa ccggcggcac
    16501 cggtatataa acgacataac taaaataaag cagtaccaat aaaaacaaga aaagcgcgac
    16561 ggccggcacg tataacgtgt cggccgtttt cgcatccagg aggcataaaa acaggccttg
    16621 gggcctgccg cgccggcagc taccatggcg gttgcacatc aaggccagca agagcacaac
    16681 aacatgtcat tgcctgcccc gcccgtgccg cccgcggccg ctcgcgccta ttggcgccgc
    16741 aatctgcgct atatcgcgct gctgctggcc gtgtggctgt tgctgaccct ggcgccggcg
    16801 ctgttcacgg gctggctgtc cttcgacttc atcggctggc cgtttccctt ctggctggcg
    16861 gcctatggcg cgccgctggc ctatctgttc atcgtcgcgg tgtatgcctg gctgatgagc
    16921 cgcgccgacc gccccgggcg ggatcgctga tgcagtccgc ccgcgggccg cgcgagttca
    16981 gcctatggct gcgccgcatc tacctgctct ataccgcggg gttctgcctg ctggtgggcc
    17041 tgctcgcgct ggccgaagtg gcgggcctgg cgcgcaactg gataggctat gtgttcctga
    17101 tcatcacggt cagcctgtat gccagcatcg gcatcctttg ccgcacctcc gaccaggtgg
    17161 agtactacgt ggcgggccgg cgcgtgccgg ctttctacaa cggcatggcg acggcggccg
    17221 actggatgtc ggtggcttcc ttcatcggcg tggcgggcac gctctatctc accggctacg
    17281 gcggcctggc ctatgtcatg gggtggacgg gcggctatgt gctggtggcg ctgctgctgg
    17341 cgccgtacct gcgcaagttc ggccagtaca ccattccgga ttttctcggc gcccggttcg
    17401 gcggcaatgt gccgcggctg gcgggcgtcg cctgcgcggt gctgtgctcg ttcacgtatc
    17461 tggtggcgca gatctacggg gtgggcatca tcaccacgcg gctcaccggc atccctttcg
    17521 agctgggcat cttcgtggcg ctgggcggca tgctggtctg ttctttcctg ggcggcatgc
    17581 gcgcggtgac ctggacccag gtggggcagt acatcatcct ggtgatcgcc tacctggtgc
    17641 cggtggtgtg gttgtcggtg aaggacaccg gttcgcccat tccgcaattg tcggcggggt
    17701 cggtgctgcg ccaggtcacc gagaaagagc tggatctgcg caacgatccg gccgagcagg
    17761 ccgtgcgtcg cctatggcgc gcaaaggccc aggaaatgac gcagcgcctg gaggccttgc
    17821 cccagtcctg ggcgcaggaa aaagaacgcc tgcgcacccg gctgtccgag cgcatcgcgg
    17881 ccgatgcgcc catggtcgag gtacgcacgc tcgagcgtga actggccgat ttcccggccg
    17941 atcccgagtc ggcgcgcgcg gcctggtcgc aagccaggtc ggtgtacgag gcgcgcggtt
    18001 cgccgcccgt gccgcacgcc cagtcttttc ccggcggcga tgcggccgag aagcgcaata
    18061 tgcgcgtcaa tttcctggcg ctggtgctgt gcctgatgct gggcacggcg ggcatgccgc
    18121 acatcctcac gcggtcctac accacgccgt cggtgggcga tgcgcggcgc tccgttttct
    18181 gggcgctgct gttcatcctg ctgctgtact tcacggcgcc ggcgctggcc ctgctggtga
    18241 agtacgaggt ctacacgcag ctggtgggca gttcgtatgc cagtttgccg gcctgggttc
    18301 atgcctgggg aacggtcgat cccaatttga tgaacgtgat cgacatcaat cacgacggca
    18361 tcgtgcagtt gggcgagatc ggcatcggcg ccgacgtggt cgtgctggcc atgcccgaga
    18421 tcggcggcct gccttatgtg atttccggcc tggtggccgc gggcgctctg gcggcggcgc
    18481 tgtcgaccgc cgacggcctg ttgctgacgc tgtccaactc cctgtcgcac gacatgttgt
    18541 accgcgtggt gtcgccgcgc atgccggccg gccggcgggt gatcgcatcc aaggtattgc
    18601 tgctgctggt ggctttcgcc gcggcctggg tggcggcgag aaagcccgcc gacatcctca
    18661 tcatggtgtc ggcggccttt tcgttcgcgg cctcgtcgtt ctttccggtg ctggtcatgg
    18721 ggatgttctg gaagcgcgcc aacaagtggg gggcgacgct ggggatggcg gtggggccgg
    18781 tggtgacctt cgcctatatg gcgtataccg acccctggct acgcgagtgg gtgttcggcg
    18841 tgccgaggac ggatccggtg agcctgtggt ggggaataca gcccatttgc gcggggctgt
    18901 tcggcgcgcc ggcggcgttc ttgacgatcg tggcggtgtc gctgttgacg cgccggccgg
    18961 atacggcgac tgaggctttg gtggattatt tgagggagcc ggcggagtcg tagtgacggt
    19021 atcagacctg catcaaaacc agcagggccc ccgacccgcc ttcgcgaggg cttgcctcgg
    19081 aaaacgccag cacgtcggtt ttttgcgcca gccaggcgcg cgccttgtcc ttgagcacgg
    19141 gcgacatgcc ttccgagcca tagcccttgc cgtgcacgat gcgcacgcac cgtatgcctt
    19201 gcgagaggca ctcgtccagg aatgaaatca cggcatggcg cgcttgttcg acgcgcaggc
    19261 cgtgcaggtc gagttcggcg cctgcgcgcc aatggccttg cttgagcttg cgggccgtgt
    19321 cgggggcggc atcggcgcgc acgtaggcgg tgccattctc ggagagcaga tgcgctacgc
    19381 cgccgtcgga aatgccggca tcggcccggc ccggtttctc gcccagggca tgggcgcggc
    19441 gtgcggccac gaccgggtcg acgggcaggg cggcaggacg gcgctcgacc aggttgtgcg
    19501 ccttgatggg cgcggcgccc tgcatggctt tgcggaaggc gtcgacgtcg tgcgccgggg
    19561 cggcctgctc gcgccgggcg cgagccgcgg cggcttcgcg ctcggcgcgc tcgcgttcct
    19621 gccgcgtctg ctcgaattcc tggtgcagct ttttcaggtc ggccaagccg cctttcgact
    19681 tactacgcat caattttctc tgctagaaag gcagttttgc cgccgggccg ccccaaggca
    19741 aaacagcccc ctcggggggc agcaagccga aggcgcggcg tgggggctta tccttccgcc
    19801 gggccgccca aaggcaaaac agccccctcg gggggcagca ccgaaggcgc ggcgtggggg
    19861 cttatccttc cagccagtgc tgcgcatcca gcgcggccat gcagccggtg gcggcgctgg
    19921 tgatggcctg gcggtagacg tagtcctgca catcgccggc ggcgaacacg ccgggcaccg
    19981 aggtcagggt agccatgccg ttgtggccgc tttgcgtgac gatgtagccg tccttcatct
    20041 tgagctggcc ttcgaagagg ccggtattgg gttggtggcc gatggcgatg aagcaaccca
    20101 tcacgtccac gtcttcggtg gcgccggttt gcaggttgcg gatgcgcgcg cccgtgacgc
    20161 cgccatcgtc gcccagcact tcatcgagca cgctgtggag cttgagcgcc atattgccgt
    20221 tggcgacctt gtccatcatg cggtcgatga ggatgggctc ggcgcggaac ttgtcgcggc
    20281 ggtggatcac ggtgaccttg cggcatagat tggacaggta cagggcttcc tcgacggccg
    20341 tgttgccgcc gccgaccacc accacgtcct tgttgcggta gaagaagccg tcgcaggtgg
    20401 cgcaggccga cacgcctcgg cccatgaagg ccgattcgct ttccaggccc aggtacttgg
    20461 ccgaggcgcc ggtggcgatg atgagcgcgt cgcaggtgta caggttgccc gtgtcgccgg
    20521 tcagcgtgaa gggacgcttg gacaggtcca ccttgtcgat gtggtcgaac accatttcgg
    20581 tttcgaatcg ctcggcgtgc ttctggaagc gctccatcag gtcgggaccc tgcacgccgt
    20641 cgacgtcggc gggccaattg tcgacctcgg tggtggtcat cagctggcct ccctgggcca
    20701 ggccggtgat cagcatgggc ttgagattgg cgc
//
LOCUS       DASWPO010000023        15850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2414637, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000023 DASWPO010000000
VERSION     DASWPO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15850
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414637"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1692)
                     /locus_tag="VGC69_01015"
     CDS             complement(295..1692)
                     /locus_tag="VGC69_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0293892.1"
                     /translation="MIQPPSPPHAVAPWLRALEPDASPYYLQIADQIAQAVGAGALRP
                     GDRLPPQRQLAQHLGVDLTTITRGYAEARHRGLLQARGAQGSFVAEPRAELARMIDLS
                     MNLPPPPLGVSLDALIQRGLNEVLARHDASMLMTYHLGGGDPASREAGAAWLAPMLGR
                     VDPQRIAVCSGAQSALAALILSQTGIGDALVTEPLVYPGLRSAAAQLGRRVVAAQADA
                     DGLLPDALERACREHGARLLYLNPTLHNPTTRTMPEGRRRDIVRVARALDLQIIEDDP
                     YWLLEDDAPPPLAQLAPERSHYVSTLSKCLTPGLRTAYALLPDAGAHERFLAALRSVV
                     LMSAQLMTGLTTQWMLDGSAARLLEGVRGEARARQALARQWLPATARVPAGGIHVWQD
                     LPARWTPHTLARVARDEGFGITPSDAFCVDGPAPNSVRLSLGGVPDRAQLTIALKKLA
                     RLLERDPAASQDIVI"
     gene            1887..2786
                     /gene="cyoA"
                     /locus_tag="VGC69_01020"
     CDS             1887..2786
                     /gene="cyoA"
                     /locus_tag="VGC69_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEY0293893.1"
                     /translation="MPLWLLAGCSQMELFTPKGSIGEQEKNLILIASGLMLLVVVPVI
                     LLTLFFAWRYRASNPRATYAPTWAHSTRIEVVVWTIPCIIVAILAVLIWRTTHELDPY
                     QPIQAEAPPVRVQVVALNWKWLFIYPDYGVASVNRLELPVDTPVAFDLTAESMMNSFF
                     IPQLGSQVYAMAGMQTKLHLLANTEGTYDGMSAAFSGPGFSDMHFDAVVTGRPAFDAW
                     VAQSRTRSPALTGDVYRALALPSHKNPVASYSNVAPGLFEMIVTQYMLGTCAPGEAPP
                     APGAPRMSMPMSRQDGGERAAME"
     gene            2792..4768
                     /gene="cyoB"
                     /locus_tag="VGC69_01025"
     CDS             2792..4768
                     /gene="cyoB"
                     /locus_tag="VGC69_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEY0293894.1"
                     /translation="MFGKLSLEVIPYHEPIIMGTLAAVMLGALLILGAITYFGKWTYL
                     WREWITSVDHKKIGAMYVILALVMLLRGFADAIMMRVQQAVAYGGAEGYLPPHHYDQI
                     FTAHGVIMIFFVATPFILGLMNVIVPLQIGARDVAYPFINSLGFWLSVMGAVLVMMSM
                     FVGDFAATGWVAYPPLSELGYSPTAGVDYYIWSLQISGLGTTLSGINFIITILRLRAP
                     GMTPMKMPVFTWTALITNILIVAVFPVLTATLALLTADRYLDMHFFTNEMGGNPMMYI
                     NLIWVWGHPEVYILILPCFGAFSEIISTFSGKPLFGYKSMVYATSSIGVLSFFVWLHH
                     FFTMGSGANVNAFFGIMTSIISIPTGVKLFNWLFTMFRGRVRYHSATLWTIGFMTTFA
                     VGGMTGVLLAVPGADFVLHNSLFLVAHFHNVIIGGVIFGCLAGMNFWFPKVFGFRLNE
                     FWGKTAFWLWLVGFLLAFMPLYILGFKGMTRRMNHYDVPGWHPYLVVALAGAVLIGLG
                     ILAILMQLAVSIRDRKQNLDLTGDPWDGRSLEWSTASPAPFYNFAHVPRIDSLEQYWD
                     DKQAGRAHAQPARYEDIHMPRNTGSGFVISVFALVMCFALVWHIWWLAAIGLVGAVAT
                     LIARSYDRDADYYVPAAEVARIENARHALLSEVA"
     gene            4770..5390
                     /gene="cyoC"
                     /locus_tag="VGC69_01030"
     CDS             4770..5390
                     /gene="cyoC"
                     /locus_tag="VGC69_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEY0293895.1"
                     /translation="MSQVTTLHPAASPRAGHHEHHEDDGSRTTYGFWVYLMSDCLIFS
                     VLFATYAVLSGNTAGGPDGRALFDLPYIFVETLLLLLSSYTFGLAVLGQAAGRARVLG
                     WLFVTFLLGAGFIGMEVHEFGEIIGRVGPQGFSAFLSAYLTLVGTHGVHVTCGLIWLL
                     VTLHQIWRYGLDGMVRRRLACLSLFWHFLDLIWICVFTFVYLRGAM"
     gene            5390..5743
                     /gene="cyoD"
                     /locus_tag="VGC69_01035"
     CDS             5390..5743
                     /gene="cyoD"
                     /locus_tag="VGC69_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEY0293896.1"
                     /translation="MASISGHAAGRGRRYATGFILSLLLTVLSFGAATLRGFSHDSML
                     GLVVVLAVVQLLVQLVYFLHLGSAKDARENTVVFTCTAMLILIIVAGSLWVMHNANLN
                     MMPTSMSVESARTRN"
     gene            5773..6228
                     /locus_tag="VGC69_01040"
     CDS             5773..6228
                     /locus_tag="VGC69_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293897.1"
                     /translation="MQALRIAAGLLRTLRPQAQAYLPAASSKAQRALLETAGFALSGS
                     FYLPGQPANAAFPAMLATLCRIPPSSVVMMHTSIRKEPGGYTREQWSVLADACKAMRH
                     FPILEISSRGLYRAGRDSAAVHGMVEREAPFMLMHLGVGRIAISVCDKL"
     gene            complement(6282..6974)
                     /locus_tag="VGC69_01045"
     CDS             complement(6282..6974)
                     /locus_tag="VGC69_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY0293898.1"
                     /translation="MSNPKLTLLTPQNCQFIIIDHQPQMAFGVQSIDRQVLKNNVVAL
                     AKGAKVFSIPTLITTVETEGFSGHTYPEILDVFPDAKLLERTSMDSWDDQKVRDGLKA
                     NGRKKVVVAGLWTEVCNNSFALEAMDQGGYEIYMVADASGGTSKEAHDYAMQRMIQAG
                     VVPVTWQQVLLEWQRDWARKETYDAVMAIVREHSGAYGMGVDYAYTMVHKAPERAKNP
                     HEVLAPVPATHV"
     gene            complement(7057..7461)
                     /locus_tag="VGC69_01050"
     CDS             complement(7057..7461)
                     /locus_tag="VGC69_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0293899.1"
                     /translation="MANPRNLPASPDLGLLFLRVAGALMLFFVHGLPKAMDYTGQLAQ
                     IEDPFGLGPHLSLWAALFAEVVCPVFIALGWHARLACLPIIVVLAIAMLFVHFGWSLE
                     QGQFGWLLLIIFITIALCGPGRWRIGKALGEA"
     gene            complement(7478..9349)
                     /locus_tag="VGC69_01055"
     CDS             complement(7478..9349)
                     /locus_tag="VGC69_01055"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0293900.1"
                     /translation="MSASLILTNGHIHTMDRSQPIAEAVAIADGKFLEVGDAQDVMRH
                     RDAKTQVIDLKGRTAIPGLNDSHEHLIRGGLNYNLELRWEGVPSLAEAMRMLKEQAAR
                     TPVPQWVRVVGGWNQFQFVEKRMPTLDEINAAAPDTPVFILHLYDRALLNRAALAAVG
                     YTKDTPNPPGAEIQRDAAGNPTGMLIARPNANLLYTALSKGPKLPLEYQANSTRQYMR
                     ELNRLGVTSTIDAGGGFQNYPDDYAVIQKLADEGNLTIRIAYNLFTQNKGGELADFQK
                     WTSMVKPGDGTDMLRHNGAGEMLVFSAADFEDFLEPRPDLPDTMEDELEKVVRLLVSN
                     RWPFRLHATYDESISRMLDVFEKVNTDIPFDGLHWIFDHAETITPRNIDRVKALGGGI
                     AIQHRMAFQGEFFVDRYGAEAARHTPPVARMLAAGVPVGAGTDATRVASYNPWTALFW
                     LVSGSTIGGLRMVDSSGRLSRDVALELWTAGSAWFSSEQDRKGRIQAGQFADLVVLTS
                     DYFSVPEEDIKAIESVLTVVGGKVVYGDQEFTKLSPPAIPVLPDWSPVKTVPGHYRLF
                     QASEDTQNAARLRALPHACAGACNVHGHGHVAAYTSKVPAADYTSFWGALGCSCFAF"
     gene            complement(9414..9689)
                     /locus_tag="VGC69_01060"
     CDS             complement(9414..9689)
                     /locus_tag="VGC69_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HEY0293901.1"
                     /translation="MTYLISLAVGILVGIIYGLLNVRSPAPPAIALVGLLGMLIGEQV
                     VPMAKRLMHGEPVSVSWLHHECVPRITGVQPKKVAAMSDKDTPSSAS"
     gene            complement(9818..10687)
                     /locus_tag="VGC69_01065"
     CDS             complement(9818..10687)
                     /locus_tag="VGC69_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0293902.1"
                     /translation="MPPHAADTPQPSHAPRIVTLKTPLFTLMRATGQAHGGLVDVPQD
                     DAHAIIIQLQDFVSHRLWQAGRLVYSGGHRQRTLAVTDLNDRYTCQHLSPYDNIRAVL
                     SRSQLEALSEEIMDKRLGQLRHVQQSSDPVAHNLVQALLPALHQPAQADAMFVEHVML
                     ALASHVMRHYGHDDEAPAATTMQPWQERRAKEYMASRLTQEVTLEDVAQACSMSRSHF
                     ARTFKKSTGMAPFEWMRHERIKRAKHMLRNSDAALICIAVECGFTDQSHFTRVFTKLT
                     GMSPRAWRDALRH"
     gene            complement(10790..11620)
                     /locus_tag="VGC69_01070"
     CDS             complement(10790..11620)
                     /locus_tag="VGC69_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0293903.1"
                     /translation="MPRNLSDDAAASTGVLQRAFSILRILAAAKGQPMRLTDIAGRAG
                     QSQATAHRVLQSLIQEDMVQQAGSSKTYQLSVGWFSLAAMAGAQHSTLRGLCRPAMLR
                     LSGMLHDTLFLLVRHGFDAICLDRIDGPFPVRSHTGDIGGRVPLGLGQAGIILLAGLP
                     EAEREEIIRFNVPRLHHLGVFDEITLRMQVRDCLQNHYVMSSGPSLYPGTTGLGVPIQ
                     DGNGQTIAALSMAAPSERISPERLPFLVEVLRKEAAAIGAQINPFDPALRRPSQFLGA
                     "
     gene            11834..12898
                     /locus_tag="VGC69_01075"
     CDS             11834..12898
                     /locus_tag="VGC69_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0293904.1"
                     /translation="MAFLRLEQVSKYYGDLCVVSGFDLAVEQGEFVSLLGPSGCGKTT
                     TLQMIAGFAEVTRGRIFLDGRDITHAKPNTRGLGIVFQSYALFPHLTVADNVAFGLAM
                     RKVARAERAGRVAEALAMVKLGAYGERYPRELSGGQRQRVALARALVIRPPVLLLDEP
                     LSNLDAKLREDMQFELRNIQRQLGTTTIMVTHDQSEALSISDRVVVMQAGRTTQIDEP
                     YRMYEHPDNEFISRFVGKTNLMQGVVARAGDKAEIRCQSQGLLVDGRGLSTGELVTVS
                     LRPEKLMPVAAGQGKLPGTVQARYFLGSQWLYEVDTALGVLTVLAPNDGRQRLYGDGD
                     ALGLDWVDDVVRVVPAQEQA"
     gene            12972..13793
                     /locus_tag="VGC69_01080"
     CDS             12972..13793
                     /locus_tag="VGC69_01080"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0293905.1"
                     /translation="MLGSLPVLLLFVALVVVPLLMTLALSFSRFDYNTGIQPGHTLAY
                     YTTVLTDSYFLGIFWRTLWISALTTLICAAIGAPEAYILSRMKKPWRSVFLLVVLSPL
                     LISLVVRAFGWSLLLGPAGPIGQVAAALGLGRLLYTPGAIIVGLVHVMLPFMIIPVWT
                     SLQKLDPSIEHAALSLNASRWQTLARVVMPQAVPGMLSGSLIVFGLSASSFAIPALLG
                     GRRLKMAATVVYDQFLTDLNWPQGAAIAMLLLVANIIIMTTYNRVLERSYRRSLG"
     gene            13800..14594
                     /locus_tag="VGC69_01085"
     CDS             13800..14594
                     /locus_tag="VGC69_01085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0293906.1"
                     /translation="MQKNSVAALVFNALVVAFILAPLAIVCWVAFTPASTLTLPYSDF
                     SLRWFKAVFADDEFMQAFRNSLWAAFLSASIASLLSVPAAVAISRHAFPGRDALGGLL
                     LSPLMIPHLVLGVALLRFFAAMQVAGSFPLLVAGHVIVITPYVMRLVLGALVGFDRSI
                     EHAALSLGASRPAVFLKVTLPLILPGVAGGWLLAFISSFDELTLSVFITSPTTVTLPV
                     RMYMYATESIDPMMAAVSALVVALTALTMVVLDRLYGLDRILVGER"
     gene            14634..14921
                     /locus_tag="VGC69_01090"
     CDS             14634..14921
                     /locus_tag="VGC69_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0293907.1"
                     /translation="MAILKRVAETGRARVAFFIDGRPAEALAGDTVLTALLTQGERLR
                     LSEFGGGPRAGFCMMGACQDCWVRQEDGARMRACSTFVQAGMRLLTAGDAA"
     gene            14918..>15850
                     /locus_tag="VGC69_01095"
     CDS             14918..>15850
                     /locus_tag="VGC69_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0293908.1"
                     /translation="MSASARPPEGRAPSQGEDVAQRQEGRSASRVVIVGAGPAGIRAA
                     QALAEAGVRPLVIDEAERPGGQIYRQPPPGFVRPKAALYGFEHRKADRLHRAMQALDG
                     RIDYLPGALAWNGTAGMLDVMQAGRNRGEPYSHLILATGATDRVLPFPGWTTPGVYTL
                     GGAQVALKFQGCTVGATVAFLGTGPLLYLVAYQYARAGARVAAVLDTAGAADHRAGLP
                     GLLRSPGLLAKGLYYLAWLRAHGVPVHTGVRPVAAQGAARVTSLVCRDAAGRAMQIEC
                     DAVAFGLGLRSETQLASLLGCQFGYSEQDRNWLPQ"
BASE COUNT         2305 a         5107 c         5590 g         2848 t
ORIGIN      
        1 ttctttttcc agcagttccg cggtgcgcag ctgcacggca aagccctggc ccaggttctg
       61 cgggcgctgg ccggcgggca ggcgctcgcc cgccacgccc gccaggcggg ccttgatgag
      121 aaagcgggcg gctgcgtcgc agccggcata gggttcggtt tcgatacgga tggtcatgag
      181 aggcgccctt cgacggcaga atggcaaggc ggcgcgcggg cgcctgtctt ggcggggaaa
      241 tcgaatcaat ccggaaaatt ttagatcacg gccttgcctt ggataacgat agcttcagat
      301 gacgatgtct tgcgaggcgg ccgggtcgcg ttcgagcagg cgggccagtt tcttgagcgc
      361 gatggtcagc tgggcgcggt cgggcacgcc gcccagcgac aggcgcacgc tgttgggcgc
      421 cgggccgtcg acgcagaagg catccgaggg cgtgatgccg aagccctcgt cgcgcgccac
      481 gcgggccagg gtgtgcggcg tccagcgggc cggcaggtct tgccagacat ggatgccgcc
      541 ggccggcacg cgtgccgtgg cgggcagcca ttgccgggcc agcgcctggc gcgcgcgcgc
      601 ttcgccgcgc acgccctcga gcagccgggc ggccgagccg tccagcatcc attgcgtggt
      661 caggcccgtc atcagctgcg ccgacatcag caccaccgaa cgcagggcgg ccaggaagcg
      721 ctcgtgcgcg ccggcgtcgg gcagcagcgc ataggccgtg cgcaggcccg gggtcaggca
      781 tttggacaag gtcgagacgt agtggctgcg ttcaggcgcc agttgggcca gcggcggcgg
      841 cgcgtcgtct tccagcagcc aataggggtc gtcttcgatg atctgcaggt ccagcgcccg
      901 cgccacccgg acgatgtcgc gccgccggcc ttcgggcatg gtgcgcgtgg tggggttgtg
      961 cagggtgggg ttgaggtaga gcaggcgggc gccgtgttcg cggcaggccc gctccagcgc
     1021 gtcgggcagc aggccgtcgg catcggcctg ggcggccacg acgcggcggc ccagctgcgc
     1081 ggcggccgag cgcaggccgg ggtagaccaa cggttcggtc accagggcgt cgccgattcc
     1141 ggtctgcgac aggatgagcg cggccagggc cgattgggcg cccgagcaga cggcgatgcg
     1201 ctgcgggtcg acccggccga gcatgggcgc gagccaggcg gcgccggcct cgcggctggc
     1261 cgggtcgccg ccgcccaggt ggtaggtcat gagcatgctg gcgtcgtgcc gggccagcac
     1321 ctcgttcaag ccgcgctgga tcagggcgtc caggctcacg cccagcggcg ggggcggcag
     1381 gttcatgctg aggtcgatca tgcgcgccag ttcggcccgg ggctcggcca cgaacgagcc
     1441 ttgcgcgccg cgggcctgca gcaggccgcg gtggcgggcc tcggcgtagc cgcgcgtgat
     1501 ggtggtgagg tccaccccca ggtgctgggc cagctggcgc tgcggcggca ggcggtcgcc
     1561 ggggcgcagg gcgccggcgc cgaccgcctg ggcgatctgg tcggcgatct gcaggtagta
     1621 ggggctggca tcgggctcga gcgcccgcag ccagggcgcc acggcgtgcg gaggagaggg
     1681 cggctggatc atgcgtttgt caacatgtat ggcttgatgt actatttgta tgcattcaat
     1741 ttatacatta atccatacat aaaatccatg tatggatggg cggcgcaggt cagggcgccg
     1801 ccaccgcccg gcgcggcgga cttgcgtaca aggtcttcat cgtggtctat cgtcgatatt
     1861 tctccgcatg gcgcctgctg ggcgccatgc cgctgtggct gctggccggc tgcagccaga
     1921 tggaattgtt cacccccaag ggcagcatcg gggagcagga aaagaacctg atcctcatcg
     1981 cgtcgggcct gatgctgctg gtggtggtgc cggtcatcct gctcacgctg ttcttcgcct
     2041 ggcgctaccg ggcatcgaac ccgcgcgcca cctacgcgcc cacctgggcg cactccaccc
     2101 gcatcgaggt ggtcgtctgg accatcccct gcatcatcgt ggccatcctg gccgtgctga
     2161 tctggcgcac cacccacgag ctggacccgt accagcccat ccaggccgag gcgccgcccg
     2221 tgcgggtgca ggtggtggcg ctgaactgga agtggctgtt catctacccc gactacggcg
     2281 tggcctcggt caaccggctg gaactgccgg tggacacgcc ggtggccttc gacctgacgg
     2341 ccgagtcgat gatgaattcc ttcttcatcc cccagctggg cagccaagtg tatgccatgg
     2401 caggtatgca gacaaagctg cacttgctgg caaacacgga aggcacgtat gacgggatgt
     2461 cggccgcttt cagcgggccc ggcttctcgg acatgcactt cgatgcggta gtgaccggcc
     2521 ggcccgcctt cgatgcctgg gtcgcccagt cgcgcacccg gtcgcccgcg ctcacgggcg
     2581 atgtctaccg ggccctggcc ctgcccagcc acaagaaccc ggtggcttcg tactcgaacg
     2641 tggcacccgg cctgttcgag atgatcgtca cccaatacat gctgggcact tgcgcgcccg
     2701 gggaggcccc gccggccccc ggcgcgccgc gcatgtccat gcccatgtcc aggcaagacg
     2761 gcggcgaacg cgccgccatg gagtaaccgc catgttcgga aagctttccc tcgaagtcat
     2821 cccctatcac gagcccatca tcatgggcac gctggccgcg gtcatgctcg gcgcgctgct
     2881 gatcctgggc gccatcacgt atttcggcaa gtggacctac ctgtggcgcg agtggatcac
     2941 ctcggtcgac cacaagaaga tcggcgcgat gtacgtgatc ctggcgctgg tgatgctgct
     3001 gcgcggcttc gccgacgcca tcatgatgcg ggtccagcag gccgtcgcct acggcggggc
     3061 cgagggctac ctgccgccgc accattacga ccagatcttc acggcgcacg gcgtgatcat
     3121 gatcttcttc gtggccacgc ccttcatcct gggcctgatg aacgtcatcg tgcccctgca
     3181 gatcggcgcg cgcgacgtgg cctacccctt catcaattcg ttgggcttct ggctgtcggt
     3241 catgggcgcg gtgctggtga tgatgtccat gttcgtcggc gacttcgccg cgacgggctg
     3301 ggtggcctac ccgcccctgt ccgagctggg gtacagcccc acggcggggg tggactacta
     3361 catctggtcg ctgcagatat cggggctggg caccacgctg tcgggcatca acttcatcat
     3421 caccatcctg cgcctgcgcg cgccgggcat gacgccgatg aagatgccgg tgttcacctg
     3481 gacggcgctc atcaccaata tcctgatcgt ggcggtgttc ccggtgctga cggccacgct
     3541 ggcgctgctg acggccgacc gctatctcga tatgcacttc ttcaccaacg agatgggcgg
     3601 caacccgatg atgtacatca acctcatctg ggtctggggc catcccgagg tgtacatcct
     3661 gatcctgccg tgcttcggcg cgttctcgga gatcatctcg accttctcgg gcaagccgct
     3721 gttcggctac aagtcgatgg tgtacgccac ctcgtcgatc ggcgtgctgt cgttcttcgt
     3781 gtggctgcac cacttcttca ccatggggtc gggggccaac gtcaatgcct tcttcggcat
     3841 catgacctcc atcatctcga tccccaccgg ggtcaagctg ttcaactggc tgttcaccat
     3901 gttccgcgga cgggtgcgct accactcggc cacgctgtgg accatcggct tcatgacgac
     3961 cttcgccgtg ggcggcatga cgggcgtgct gctggccgtg ccgggcgccg acttcgtgct
     4021 gcacaacagc ctgttcctgg tggcgcactt tcacaacgtg atcatcggcg gggtgatctt
     4081 cggctgcctg gcggggatga acttctggtt tcccaaggtg ttcggcttca ggctcaacga
     4141 gttctggggc aagacggcct tctggctctg gctggtgggc tttctgctgg ccttcatgcc
     4201 gttgtacatc ctgggcttca agggcatgac gcgccgcatg aaccactacg acgtgcccgg
     4261 ctggcaccct tacctggtgg tggccctggc gggcgcggtg ctgatcggcc tgggcatcct
     4321 ggccatcttg atgcagctgg ccgtgagcat ccgcgaccgc aagcagaacc tggacctcac
     4381 cggcgacccc tgggacggcc gcagcctgga atggtcgacc gcgtcgccgg cgccgttcta
     4441 caacttcgcc cacgtgccgc gcatcgattc gctcgaacag tactgggacg acaagcaggc
     4501 cggccgcgcc cacgcgcagc ccgcgcgcta cgaagacatc cacatgccgc gcaataccgg
     4561 ttcgggcttc gtcatatcgg tgttcgcgct ggtgatgtgc ttcgcgctgg tctggcacat
     4621 ctggtggctg gccgcgatcg gcctggtcgg ggcggtcgcc accctgatcg cgcgatccta
     4681 cgaccgcgac gccgactatt acgtccccgc ggccgaagtc gcgcgcatcg aaaacgcgcg
     4741 ccatgccctg ttgagcgagg tggcctgata tgagccaagt cacgacactg cacccggcgg
     4801 cgtccccgcg cgccgggcat catgagcacc atgaagacga cggatccagg accacctacg
     4861 gtttctgggt ctacctgatg agcgactgcc tgattttctc ggtgctgttc gcgacctatg
     4921 ccgtgctgtc cggcaacacg gccggcgggc ccgacggccg cgcgctgttc gacctgccct
     4981 acattttcgt cgagaccctg ctgcttttgc tcagcagcta caccttcggc ctggccgtgc
     5041 tggggcaggc cgccggccgc gcccgggtgc tgggctggct gttcgtcact ttcctgctgg
     5101 gggcgggctt catcggcatg gaggtccatg agttcggcga gatcatcggc cgcgtcggcc
     5161 cgcaagggtt cagcgccttc ctgtcggcct acctgaccct ggtgggcacc cacggggtgc
     5221 acgtgacctg cggcctgatc tggctgctgg tcacgctgca ccagatctgg cgctacgggc
     5281 tcgacggcat ggtgcgccgc cgcctggcct gcctgagcct gttctggcac ttcctggatc
     5341 tgatctggat ctgcgtgttc accttcgtct atctacgggg agcaatgtaa tggcctcgat
     5401 ctcgggtcac gccgccggtc gcggccggcg ctatgccacc ggtttcatcc tgtcgctgct
     5461 gttgacggtg ctgtcgttcg gcgccgccac gctgcgcggg ttttcccacg actcgatgct
     5521 ggggctggtg gtggtcctgg cggtggtcca gctgctggtg cagctggttt atttcctgca
     5581 tctgggcagc gccaaggatg cgcgcgagaa caccgtcgtc ttcacttgca cggccatgct
     5641 catcctgatc attgtcgcgg ggtcgctatg ggttatgcac aacgccaatc tgaatatgat
     5701 gccgacctcg atgtcggtcg agagcgcgcg gacgcggaac tgaacgtcgt tccgacgcgc
     5761 cctttcatcg gcatgcaggc cttgcgcatc gccgcggggc tgttgcgcac gctgcgcccg
     5821 caggcgcagg cgtacctgcc ggcggccagc agcaaagcgc agcgcgccct gctggagacg
     5881 gcgggtttcg cgctgagcgg ttcgttctac ctgccggggc agccggcgaa cgcggccttt
     5941 ccggcgatgc tggcgacgct gtgccgcatt ccgccgtcca gcgtggtgat gatgcacacc
     6001 tcgatacgca aggagcccgg cggctatacc cgcgagcagt ggtccgtgct ggccgatgcc
     6061 tgcaaggcca tgcggcactt tcccattttg gagatcagca gccgcggcct gtaccgcgcg
     6121 ggccgcgaca gcgccgccgt gcacggcatg gtcgagcgcg aggcgccgtt catgctgatg
     6181 cacctgggcg tggggcgcat cgccatttcg gtttgcgaca agctctgacg tgcctggaaa
     6241 agccatcctg gccaggatgg cttgcccgaa tcgggatcgg cttacacgtg cgtggccggc
     6301 acgggggcca gcacttcgtg cgggttcttg gcgcgctcgg gggccttgtg caccatggtg
     6361 taggcatagt ccacgcccat gccgtaggcg ccggaatgct cgcgcacgat ggccatgacg
     6421 gcgtcgtagg tttccttgcg ggcccagtcg cgctgccatt cgagcagcac ctgctgccag
     6481 gtcacgggca cgacgccggc ctggatcatg cgctgcatgg cgtagtcgtg ggcctccttg
     6541 gaagtgccgc ccgaggcgtc ggccaccatg tagatctcgt agccgccctg gtccatcgct
     6601 tccagcgcga agctgttgtt gcacacttcc gtccacaggc cggccaccac caccttcttg
     6661 cggccgttgg ccttcaggcc gtcgcgcacc ttctggtcgt cccaggagtc catcgaggtg
     6721 cgctcgagca gcttcgcgtc ggggaacacg tcgaggatct cggggtaggt gtggcccgag
     6781 aagccttcgg tctccaccgt ggtgatgagg gtggggatgc tgaagacctt ggcgcccttg
     6841 gccagggcca ccacattgtt cttcaggacc tggcggtcga tggactgcac gccgaaagcc
     6901 atctgcggct ggtggtcgat gatgatgaac tggcagtttt gcggcgtcag cagcgtgagt
     6961 ttgggattcg acatggtcat gctccagtga ggtgcgagcc ccgcgacttg gggccggagt
     7021 tgccagccta tggctgtacg ggtttgcgga ttcgggtcat gcctcgccga gggccttgcc
     7081 tatgcgccag cggcccgggc cgcacagcgc gatggtgatg aagatgatca gcagcagcca
     7141 gccgaactgg ccctgctcga gggaccagcc gaagtgcacg aagagcatgg caatggccag
     7201 caccacgatg atgggcaggc aggccagccg cgcatgccag cccagcgcga tgaagacggg
     7261 gcagacgacc tcggcgaaga gcgccgccca caggctgaga tgcggcccca ggccgaaggg
     7321 gtcctcgatc tgggcgagct ggccggtgta gtccatggcc ttgggcagcc cgtgcacgaa
     7381 gaacagcatc aaggcgccgg cgacgcgcag gaacagcagc cccaggtcgg ggctggcggg
     7441 caggttgcgg gggttggcca tggcggactc ggtcggctca gaaggcgaag cagctgcagc
     7501 ccagggcgcc ccagaagctg gtgtagtcgg ccgccggcac cttggacgtg taggccgcca
     7561 cgtggccgtg gccgtgcaca ttgcaggcgc ccgcgcaggc gtgcggcagg gcgcgcagcc
     7621 tggcggcgtt ctgggtgtct tcgctggcct ggaacaggcg gtagtggccc ggcacggtct
     7681 ttaccggcga ccagtccggc agcaccggga tggccggcgg cgacagcttg gtgaactcct
     7741 ggtcgccgta gacgaccttg ccgcccacga cggtcagcac cgactcgatg gccttgatgt
     7801 cttcctcggg cacgctgaag tagtcggacg tgagcacgac cagatcggcg aactggccgg
     7861 cctggatgcg gcctttcctg tcctgttcgc tggagaacca ggcgctgccg gccgtccaca
     7921 gttccagggc gacgtcgcgc gacaggcggc ctgaggagtc gaccatgcgc aggccgccga
     7981 tggtgctgcc cgagaccagc cagaacagcg ccgtccaggg gttgtagctg gccacgcggg
     8041 tggcgtcggt gccggcgccc accggcacgc ccgctgccag catgcgggcc acgggcgggg
     8101 tgtgcctggc ggcctcggcg ccgtagcggt cgacgaagaa ctcgccctgg aaggccatgc
     8161 ggtgctggat ggcgatgcca ccgcccaggg ccttcacgcg atcgatattg cgtggggtga
     8221 tggtctcggc gtggtcgaag atccagtgca ggccgtcgaa ggggatgtct gtgttcacct
     8281 tctcgaacac gtccagcatg cggctgatgg actcgtcgta ggtggcgtgc aggcggaagg
     8341 gccagcggtt gctcaccagg aggcgcacca ccttctcgag ttcgtcttcc atggtgtccg
     8401 gcaggtcggg gcgcggctcg aggaagtcct cgaagtcggc cgccgagaac accagcattt
     8461 cgcccgcgcc gttgtggcgc agcatgtcgg tgccgtcgcc cggcttgacc atgctggtcc
     8521 atttctggaa gtcggccagt tcgccgcctt tgttctgcgt gaacagattg taggcgatgc
     8581 ggatggtgag gttgccttcg tcggccagct tctggatgac ggcgtagtcg tcgggatagt
     8641 tctggaagcc gccgcccgcg tcgatggtgc tggtcacgcc caggcggttg agctcgcgca
     8701 tgtattggcg ggtggagttg gcctggtatt cgagcggcag cttggggccc ttggacagcg
     8761 ccgtgtacag caggttggcg ttggggcggg cgatcagcat gccggtgggg ttgcccgcgg
     8821 catcgcgctg gatttccgcg cccggcgggt tgggcgtgtc cttggtgtag ccgacggcgg
     8881 ccagcgcggc gcgattgagc aaggcgcggt cgtacaggtg caggatgaac accggcgtgt
     8941 cgggcgcggc ggcgttgatc tcgtcgagcg tgggcatgcg cttctcgacg aactggaact
     9001 ggttccagcc gccgaccacg cgcacccatt gcggcacggg cgtgcgcgcg gcctgctctt
     9061 tgagcatgcg catggcttcg gccagcgagg gcacgccttc ccagcgcagc tccaggttgt
     9121 agttcaggcc gccgcggatc aggtgctcgt gcgagtcgtt caggccgggg atggcggtgc
     9181 ggcccttcag gtcgatgacc tgggtcttgg cgtcgcggtg ccgcatcacg tcctgggcgt
     9241 cgccgacctc caggaacttg ccgtcggcga tggccacggc ttctgcgatg ggctggctgc
     9301 gatccatggt gtggatgtgg ccgttggtca ggatcaggga tgcgctcatg tcggtcgtcc
     9361 tgcaggaaga agtgaaccgg caggcgccat gccggcgcct ggccggaagg gaattacgag
     9421 gcggaggaag gcgtgtcctt gtcggacatc gcggcaacct tcttgggttg gacgcccgtg
     9481 atcctgggca cgcattcgtg gtgcagccag ctcaccgaga cgggctcgcc gtgcatgagc
     9541 cgcttggcca tgggcacgac ctgttcgccg atcagcatgc ccagcagacc caccagggca
     9601 atggccggcg gcgccgggga gcgcacgttg agcaggccat agatgatgcc gacgagtatg
     9661 cctacagcca gggagatgag gtaagtcatg gcgatttcct tgagcatggc gggcgcgccg
     9721 caattgccac tatgagccat cgccgagacg gcaagatagg atttgcgtgc cgtcgttttg
     9781 gtatatccgt gcccgtcgcg ggcggcggac tgcgcgccta gtggcgcaag gcgtcgcgcc
     9841 aggcgcgcgg gctcatgccc gtcagcttgg tgaagacgcg ggtgaaatgg ctctggtcgg
     9901 tgaagccgca ttcgacggcg atgcagatca gggcggcatc gctgtttctg agcatgtgct
     9961 tggcgcgctt gatgcgttcg tggcgcatcc attcgaaggg cgccatgccg gtgcttttct
    10021 tgaaggtgcg ggcgaaatgg ctgcgcgaca tgctgcaggc ctgcgccacg tcttcgagcg
    10081 tcacttcctg ggtcaggcgg ctggccatgt attccttggc gcggcgctcc tgccagggtt
    10141 gcatggtggt cgcggcgggg gcttcgtcgt cgtggccata gtggcgcatc acgtgcgagg
    10201 ccagggccag catgacgtgt tccacgaaca tggcatcggc ctgggccggc tggtgcagcg
    10261 cgggcagcag ggcctgcacg aggttgtggg ccaccgggtc ggaggactgc tgtacgtgcc
    10321 tgagctgacc gaggcgtttg tccatgattt cctccgagag ggcttccagt tggctgcggc
    10381 tcagcacggc gcggatattg tcgtagggcg acaggtgctg gcaggtgtag cggtcgttca
    10441 gatcggtgac cgccagggtt ctttgcctgt gtccgccaga atagacgagg cgccccgctt
    10501 gccacagccg atgtgaaaca aaatcttgca gctggatgat gatggcgtgg gcgtcgtcct
    10561 gcgggacgtc gaccaggccg ccatgcgcct ggccggtcgc ccgcatgagg gtgaacagcg
    10621 gcgtcttcag ggtgacgata cgcggtgcgt gcgagggctg gggcgtatcg gcggcatgcg
    10681 ggggcatcgg gcacctcgaa gggcatacac atcggccatg gggcagggaa ccacaggccg
    10741 attataggga ggggttgctt cagggacatg cccgtcagca aacaagccgt caggcgccca
    10801 ggaactggct cggcctgcgc aacgccgggt cgaaggggtt gatctgcgcg ccgatggccg
    10861 cggcttcctt gcgcaggact tccaccagga aaggcaggcg ttccgggctg atgcgctcgg
    10921 atggcgccgc catgctcagg gcggcgatcg tctggccgtt gccgtcctgg atggggacgc
    10981 cgagcccggt ggtgccgggg tacagcgagg ggcccgagct catgacgtag tggttttgca
    11041 ggcagtcgcg gacctgcatg cgcagggtga tttcatcgaa gacgcccagg tggtgcagcc
    11101 gcggcacatt gaagcggatg atttcttcgc gctcggcctc gggcaggccg gccagcagga
    11161 tgatcccggc ctggcccagg cccaggggca cgcgcccgcc gatatccccg gtatgcgaac
    11221 gcacggggaa ggggccgtcg atgcggtcca ggcaaatggc gtcaaagcca tggcgcacca
    11281 gcaggaacag ggtgtcgtgc agcatgcccg acaggcgcag catggccggc cggcacaggc
    11341 cgcgcaaggt ggagtgctgc gcgccggcca tggccgccag cgagaaccag cccacgctga
    11401 gctggtaggt cttgctcgag ccggcctgct ggaccatgtc ttcctggatg agcgactgca
    11461 ggacgcggtg ggcggtggcc tgcgactggc cggcccggcc ggcgatatcg gtcaggcgca
    11521 tgggctgccc cttggccgcg gccaggatgc gcaggatgga aaaggcgcgc tgcagcacgc
    11581 cggtgctggc ggccgcgtcg tcggaaaggt tgcgtggcat agggttcttc gggatctggc
    11641 tggggaaata ctaaaaataa aaccatcatc aagcaattat attttcactt gatggaattt
    11701 tattaaaata aattccttta aatggaatgc ccgtattgac cgtcgtcgaa gccggttcct
    11761 acactgcctg caaataaaag ggcgtgggct tacgggcccg ccccggcaag ccgcaaggcg
    11821 attcgaggcg gaaatggctt ttcttcgact ggagcaagtc tccaagtact acggcgacct
    11881 gtgcgtggtt tccgggttcg acctggcggt ggaacagggg gagttcgtct ccctgctggg
    11941 cccgtcgggc tgcggcaaga cgaccaccct gcagatgatc gcgggcttcg ccgaggtgac
    12001 gcgcggccgc atcttcctcg acgggcgcga catcacccat gccaagccca acacgcgcgg
    12061 cctgggcatc gtcttccaga gctatgcctt gtttccgcac ctgacggtcg ccgacaacgt
    12121 ggccttcggc ctggccatgc gcaaagtggc gcgcgccgag cgcgccggac gcgtggccga
    12181 ggcgctggcc atggtcaagc tcggcgccta cggcgagcgc tacccccgcg aattgtcggg
    12241 cggacagcgg cagcgggtcg ccctggcgcg cgcgctggtc atccgtccgc ccgtgctgct
    12301 gctcgacgaa cccttgtcca atctggacgc caagctgcgc gaagacatgc agttcgaatt
    12361 gcgcaacatc cagcgccagc tgggcaccac caccatcatg gtcacgcacg accagtccga
    12421 ggccctgtcg atcagcgacc gcgtggtggt catgcaggcc gggcgcacga cccagatcga
    12481 cgagccctac cgcatgtacg agcaccccga caacgagttc atctcgcgct tcgtgggcaa
    12541 gaccaacctg atgcagggcg tggtggcgcg ggccggcgac aaggcggaga tccgctgcca
    12601 gtcgcagggc ctgctggtcg acggccgggg cctgagcacg ggcgagctcg tgacggtttc
    12661 cctgcgtccc gaaaagctca tgcccgtggc ggcggggcag ggcaaattgc cgggcacggt
    12721 ccaggcgcgc tacttcctgg gcagccagtg gctgtacgaa gtggacaccg cgctgggcgt
    12781 gctgaccgtg ctggcgccca acgacggccg gcagcggctc tatggcgacg gcgacgcgct
    12841 gggcctggat tgggtcgacg acgtggtgcg cgtggtgccg gcgcaggagc aggcctgaca
    12901 tggcgcgcgc cgcccagtcc cgccaggcgc cggtcgcggc gcaggtgccg cggcgcgaca
    12961 gcctgccggc catgctcggg tccttgcccg tcctgctcct gttcgtggcc ctggtcgtcg
    13021 tgccgctgct gatgacgctg gcgctgtcgt tcagccgctt cgactacaac accggcatcc
    13081 agcccggcca cacgctggcc tactacacca cggtgctgac ggacagttat ttcctgggca
    13141 ttttctggcg cacgctgtgg atctccgcgc tgaccacctt gatctgcgcg gcgatcggcg
    13201 cgcccgaggc ctatatcctc agccgcatga agaagccctg gcgctccgtc ttcctgctgg
    13261 tggtgctgtc gcccttgctg atctccctgg tggtgcgcgc cttcgggtgg agcctgctgc
    13321 tcgggccggc cgggcccatc gggcaggtcg ccgccgcgct gggcctggga cgattgctgt
    13381 acacgcccgg agccatcatc gtgggcctgg tgcacgtgat gctgcccttc atgatcatcc
    13441 cggtctggac ctcgctgcag aagctcgacc ccagcatcga gcacgcggcg ctgtcgctca
    13501 atgcctcgcg ctggcagacc ctggcgcgcg tggtgatgcc gcaggcggtg cccggcatgc
    13561 tgtcgggcag cctgatcgtg ttcggcctga gcgcgagttc gttcgccatt ccggccctgc
    13621 tgggcgggcg gcggctgaag atggccgcca cggtggtcta cgaccaattc ctcaccgacc
    13681 tgaactggcc gcagggcgcc gccatcgcca tgctgctgct ggtggccaac atcatcatca
    13741 tgacgacgta caaccgggtg ctcgagcgct cgtaccgtcg cagcctgggc tgaaccctca
    13801 tgcagaaaaa cagtgtcgcc gcgctggtgt tcaatgccct ggtcgtggcc ttcatcctgg
    13861 cgccgctggc catcgtctgc tgggtggcct tcacgccggc ctccaccttg acgctgccct
    13921 actcggattt ctcgctgcgc tggttcaagg cggtgttcgc cgatgacgaa ttcatgcagg
    13981 cctttcgcaa cagcctgtgg gcggccttcc tgtcggcgtc gatcgccagc ctgctgtcgg
    14041 tgccggccgc cgtggccatc tcgcgccatg ccttcccggg ccgcgatgcg ctcggcggcc
    14101 tcctgctgtc gccgctgatg atcccgcacc tggtgctggg cgtggcgctg ctgcggttct
    14161 tcgccgccat gcaggtggcc ggcagcttcc cgctgctggt ggcggggcac gtcatcgtca
    14221 tcacgcccta tgtcatgcgc ctggtgctgg gggcgctggt gggcttcgac cgctccatcg
    14281 agcatgccgc cctgtcgctg ggcgcgagcc ggcccgcggt gttcctgaag gtcacgctgc
    14341 ccctgatcct gcccggcgtg gcgggcggct ggctgctggc cttcatcagc agcttcgacg
    14401 aactgacgct gtccgtgttc atcacctcgc cgaccaccgt gacgctgccg gtgcgcatgt
    14461 acatgtacgc caccgaatcc atcgacccca tgatggccgc cgtgtcggcg ctggtcgttg
    14521 ccctgacggc cttgaccatg gtggtgctgg accggctcta cggactggac cgcatcctgg
    14581 tgggcgagcg ctgagcgggc gcggatcatg atgcaacgaa ggaagtcttt ccgatggcga
    14641 tattgaagcg ggtggcggaa accggccgcg cgcgcgtggc gtttttcatc gacggccggc
    14701 cggccgaagc cctggcgggc gatacggtgc tgacagccct gctgacgcag ggcgagcgcc
    14761 tgcgcctgtc cgagttcggc ggcggcccgc gggcgggctt ctgcatgatg ggcgcctgcc
    14821 aggactgctg ggtgcggcag gaggacggcg cccgcatgcg cgcgtgctcg accttcgtgc
    14881 aggccggcat gcggctgctg acggcggggg atgcggcatg agcgcgtccg cccggccgcc
    14941 cgaaggacgc gctccctccc agggggagga tgtcgcgcag cgacaggagg gccgttccgc
    15001 ttcccgggtc gtcatcgtgg gggcggggcc ggccggcatc cgcgcggcgc aggcgctggc
    15061 cgaggccggc gtgcggccgc tggtgatcga cgaggccgag cgcccgggcg ggcagatcta
    15121 ccgccagccg ccgccaggct tcgtgcgccc gaaggcagcg ctgtacggtt tcgaacaccg
    15181 caaggccgac cgcctgcatc gcgccatgca ggcgctggac ggccgcatcg attacctgcc
    15241 gggcgcgctg gcctggaacg gcaccgcggg catgctcgac gtgatgcagg ccggccgcaa
    15301 ccgcggcgag ccttatagcc atctcatcct ggcgacgggc gcgaccgatc gggtgctgcc
    15361 tttcccgggc tggaccacgc cgggcgtcta taccctgggg ggcgcgcagg tcgcgctcaa
    15421 attccagggc tgcacggtcg gcgccacggt ggctttcctg ggaaccgggc cgctcctgta
    15481 cctggtggcg tatcaatatg cccgcgccgg cgcccgggtg gccgcggtgc tggatacggc
    15541 cggcgcggcc gaccatcggg ccggcttgcc gggcctgctg cgctcgcccg ggctgctggc
    15601 caagggcctg tattacctgg cctggctgcg cgcccacggc gtgccggtgc acacgggcgt
    15661 gcgcccggtg gccgcgcagg gcgcggcgcg cgtgacgagc ctggtctgcc gggacgcggc
    15721 ggggcgcgcc atgcagatcg aatgcgatgc cgtggccttt ggcctgggcc tgcgttcgga
    15781 aacccagctg gccagcctgc tgggctgcca gttcggctac agcgagcagg accgcaactg
    15841 gttgccgcag
//
LOCUS       DASWPO010000024         8850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_720209, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000024 DASWPO010000000
VERSION     DASWPO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720209"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VGC69_01100"
     CDS             <1..699
                     /locus_tag="VGC69_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0293909.1"
                     /translation="RSVSFEFRLRASTEHNPRWMLGFWLGSLLTALGQGAALGRIATD
                     YQMDVGYVGFAAFVGLCAVAAYALLGACWLVMRVEGELQRRAINWARHTVRWTAVGMV
                     AIAVTLGLANSGVFSKWSDVDHLGFTASLWVVMLACFVGLEVALARLASKPDQSRSWL
                     PFVLCVLLYVLMLGGLAYSFFPYLVLDDMTLWDSAGSVASMRMALAGVIVGVPVVLVF
                     NVLAYRTVFGKARG"
     gene            complement(712..2112)
                     /locus_tag="VGC69_01105"
     CDS             complement(712..2112)
                     /locus_tag="VGC69_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0293910.1"
                     /translation="MRFLRYFVPRSLRARLILLVLGAVLLAQASTLTAVSYLRDKFLD
                     EVAIDYVVTTIRTLRAALAQIPAEDRADFVRVASQGQWHMWSRVLPPDARLQRRWDRR
                     WHEHEPPPPPPEFDDVRETMRSPVRELNQRLNDGTRVALSRGPAPEVFISLAPDPTFE
                     DMPQLREWLVIPIERLNPTFSTPLIAFWLGGMGLVLVLSVGFSWHITRPMTRLAEAAD
                     RLAAGQPQRVEPAGPHEIRVVGERFNAMLDALAESDSVRRTLLSGLPHDLKGPLSRMW
                     LRIEMADDSAMKEGLRKDLQDMQHMVDQFIGFVRGTDPAGYNYAPLSLPEWLAERVHA
                     WRDAGTDITLAEPEDAAMSINGDAVALGRLLDNLIGNSLHHGAAPIDVVLHQEDNQAV
                     LDVSDHGPGIPTDRRQEALRPFARLDDARTRSGNVGLGLALAEAIALAHHGTLELLDA
                     EGGGLLVRIKLPLTQA"
     gene            complement(2112..2810)
                     /locus_tag="VGC69_01110"
     CDS             complement(2112..2810)
                     /locus_tag="VGC69_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0293911.1"
                     /translation="MDPTHTKLLVVDDDPALRQLLADYLNRHGYDTLLAPDASDLSAR
                     ITRYAPDLLVLDRMLPGGDGADACRRLREQGEDIPVILLTARDEAVDRIIGLEAGADD
                     YLGKPFDPRELLARIEAVLRRKRGPSALTKDAPVSFGPFVFDPATRQLMRDDIQVKLT
                     GGEINLLEALVKNAGKPLSRERLLALARDDDGERNDRAIDIAILRLRRAIEDDPKQPR
                     WIQTVWGIGYRFSP"
     gene            3083..4900
                     /gene="recQ"
                     /locus_tag="VGC69_01115"
     CDS             3083..4900
                     /gene="recQ"
                     /locus_tag="VGC69_01115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEY0293912.1"
                     /translation="MSDPRALDVLRRVFGYESFRGEQQAIVDHVIGGGDALVLMPTGG
                     GKSLCYQVPSLVREGTGVVVSPLIALMQDQVDALTELGVRAAFLNSTQDWQQVREVEQ
                     AFVNGELDLLYVAPERLMTDRCLQLLERGRIALFAIDEAHCVSQWGHDFRPEYLQLSM
                     LHERWPDVPRIALTATATAMTRAEIAQRLALDTAHHFVASFDRPNIRYRIVEKNEVRR
                     QLLDLIRSEHAGDSGVVYGLSRNRVEETANFLCEHGINALPYHAGLSAAVRASNQSRF
                     LREDGIVMVATIAFGMGIDKPDVRFVAHIDLPKSVEGYYQETGRAGRDGLPATAWLAY
                     GLQDVVQQRRMIDESPGDEVFRRRLGQNLDAMLGLCETVQCRRVRLLAYFGQTITACG
                     NCDVCLEPPEAWDGTVAAQKVLSAVYRLQQRGQRYGAGHLIDILRGKSTDRTRQNDHE
                     SLSVFGVGKDLSEQTWRGVLRQLLAQGLLMVDGEGYGTFALTEASREVLKGERKIMLR
                     RDLEKKEKAPRGGTRARAPVVDLPAEAVPLFETLRAWRGEVAKNHGVPAYVIFHDATL
                     REIAMARPASLDVLGHISGVGARKLEAYGEEILRCLQAG"
     gene            5087..6154
                     /locus_tag="VGC69_01120"
     CDS             5087..6154
                     /locus_tag="VGC69_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0293913.1"
                     /translation="MFAFPRIPRRAQLAARISRRLSRQAVAAACLGGSLLAVCQPGLA
                     QAAQPLVKVRYEEVVRSVLYLPMYVALHRNLFKEAGLDVGMKTSQGTDKGMAALLSGS
                     ADIVLIGPEASVYVAGSESPVKPRIFAGLTATDGFLLMSREPAPAGGFDWSRLKGKNI
                     MSFRPGSNPDVFLETALRKHGLDPKQDVKLVNNIGPAARMGAWLAGQNDYATFLEPEA
                     GALEKDGKGYVLASVGAEAGPVDYTVFTTTDTYIRQHPEVLKAWTQVIAQADRIVQSA
                     PADQLAADVAEFFPGITQPELAHAIERYRTYHLWKATPAVTAQAIETLQDMLVTSGVL
                     EPGKRVPYDQLVVGDFTAQVQ"
     gene            6151..6939
                     /locus_tag="VGC69_01125"
     CDS             6151..6939
                     /locus_tag="VGC69_01125"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0293914.1"
                     /translation="MMRDDARIEVRNVSLRYFTQQGETLALDDISLSIAPGEFVSIIG
                     QSGCGKSTLLSLIAGIMPPSSGSVRVDGRPVDGPTPRVGYMLQQDYLYEWRSILDNAL
                     LGAQIQKRDMRQARERAVRLLQKCGMGDFLHHYPRQLSGGMRQRVALARTLATDPDVV
                     LLDEPFSALDSQTRLAIADEVVDVLREAGKSVVLVTHDIGEAISMTDRVVVLSRRPGR
                     IKSQHRMDFPSLGRRPKPFEARALPEFNSYFSSLWEELDVHVEG"
     gene            6944..7795
                     /locus_tag="VGC69_01130"
     CDS             6944..7795
                     /locus_tag="VGC69_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0293915.1"
                     /translation="MRTEIHAASAAAAGRTAARPAISRDYARWQQRQRRRRAGVLIAR
                     LLLLALFFGLWELLARMQWVNPLLTSYPSALWPTFLQLMREGHLLVHTWATLRATLVG
                     FVLSMVLGVVIAAGLWWSPFASKVLDPFLVVANAMPKIAFVPIFYIWLGSDYSIYGMA
                     VAIAVFVTIMVAYEGFQAIDPNKIKLAVTLGASRAQVLRLVVLPGSVPTLISAMKMNV
                     GLSLVGVIVGEFQSANEGLGFLIVNGSQVFKLNIVMTAIVILALLSALMYFVIARLEA
                     ALAKRYA"
     gene            7813..8478
                     /locus_tag="VGC69_01135"
     CDS             7813..8478
                     /locus_tag="VGC69_01135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY0293916.1"
                     /translation="MEFPDWVVDRVVARQGALHPYPRFDASSTAFVIVDMQRYFTQPG
                     YQGECPASRDTFAAVNRLAAALRAGGGSVVWVQTNADGADGFWSHHHAAMLTPERSAR
                     RLRELSASSPGFEIAPGLDVQPQDARVVKRCYSALVPGSSDLDAVLKARGIRTVLVGG
                     TVTNVCCESTARDAMMLDYATVMVDDALSAVTQAEHVNALHNWILFFGDVLSADETIA
                     RLG"
     gene            complement(8475..>8850)
                     /locus_tag="VGC69_01140"
     CDS             complement(8475..>8850)
                     /locus_tag="VGC69_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEY0293917.1"
                     /translation="IANRLLRRVRDYAEVKASGTIDHDVAGRALAMLEVDPQGLDLMD
                     RKLLEAVVHKFDGGPVGVDSLAAAIGEERDTIEDVIEPYLIQQGYLQRTPRGRMATQM
                     TWRHLGLTPPAARTAGDSSELF"
BASE COUNT         1323 a         2810 c         3195 g         1522 t
ORIGIN      
        1 cgcagcgtgt cgttcgagtt ccgcctgcgc gccagcaccg agcacaaccc gcgctggatg
       61 ctgggtttct ggctggggtc gctgctgacc gcgctggggc agggcgcggc gctgggccgc
      121 atcgccacgg actaccagat ggacgtgggc tatgtgggct tcgccgcgtt cgtgggcctg
      181 tgcgcggtgg cggcctatgc cctgctgggc gcctgctggc tggtgatgcg ggtcgagggc
      241 gagctgcagc gccgcgcgat caactgggca cgccacacgg tgcgctggac ggccgtgggc
      301 atggtggcga tcgcggtgac gctggggctg gcgaactcgg gggtgttctc caagtggagc
      361 gacgtggatc atctcgggtt cacggcgtcg ctgtgggtgg tgatgctggc gtgcttcgtg
      421 ggcctggagg tcgcgctggc caggctggcg tccaagcccg accagtcgcg cagttggctg
      481 ccgttcgtct tgtgcgtgct gctgtacgtg ctgatgctgg gcggcctggc ctacagcttc
      541 ttcccgtacc tggtgctcga tgacatgacg ctgtgggaca gcgcggggtc ggtggcctcg
      601 atgcgcatgg ccctggccgg cgtgatcgtc ggcgtgccgg tggtgctggt gttcaacgtg
      661 ctggcctacc gcacggtgtt cggcaaggcg cgcgggtagc cgccgccggt cctaggcttg
      721 cgtcaagggc agcttgatgc gcaccagcaa tccgccgcct tcggcatcga ggagttccag
      781 cgtgccgtga tgcgcgaggg cgatggcttc ggccagggcc aggcccaggc cgacgttgcc
      841 ggagcgggtg cgggcatcgt ccaggcgggc aaaggggcgc aaggcttcct ggcggcggtc
      901 ggtcggaatg ccggggccgt ggtcggagac gtcgagcacg gcctggttgt cttcctggtg
      961 cagaaccacg tcgatgggcg cggcgccgtg gtgcagggaa ttgccgatca gattgtcgag
     1021 caggcggccc agggcgacgg catcgccatt gatgctcatg gcggcatctt ccggctcggc
     1081 cagggtgatg tcggtgccgg catcgcgcca ggcgtgcacg cgctcggcca gccactcggg
     1141 cagcgagagc ggcgcgtagt tgtagccggc cgggtcggtg ccgcgcacga agccgatgaa
     1201 ctggtcgacc atgtgctgca tgtcctgcag gtccttgcgc aggccttcct tcatggccga
     1261 gtcgtcggcc atctcgatgc gcagccacat gcgcgacagc gggcccttga ggtcgtgcgg
     1321 caggcccgag agcagcgtgc ggcgcaccga gtcggattcg gccagggcgt cgagcatggc
     1381 gttgaagcgc tcgcccacca cgcggatctc atgcgggccg gccggttcga cgcgctgcgg
     1441 ctggccggcg gccagccggt ccgcggcctc ggccaggcgg gtcatgggcc gggtgatgtg
     1501 ccaggagaag cccaccgaca ggaccagcac cagccccatg ccgccgagcc agaaggcgat
     1561 caggggcgtg gaaaacgtgg ggttcaggcg ctcgatggga atgaccagcc actcgcgcaa
     1621 ttgcggcatg tcttcgaagg tggggtcggg cgccagcgag atgaagacct cgggcgccgg
     1681 gccgcgcgac agcgccacgc gcgtgccgtc gttcaggcgc tggttgagct cgcgcaccgg
     1741 gctgcgcatg gtctcgcgga catcgtcgaa ttcgggcggc ggcggaggcg gctcgtgttc
     1801 gtgccagcgg cggtcccagc gccgctgcag gcgggcatcc ggcggcagca cgcgcgacca
     1861 catatgccat tggccctgcg aggccacgcg cacgaaatcg gcgcgatcct cggcggggat
     1921 ctgcgcgagg gcggcgcgca gggtgcggat ggtggtgacc acgtagtcga tggccacttc
     1981 atctaggaat ttgtcgcgca ggtaggaaac ggcggtgagc gtggaggcct gggccagcag
     2041 cacggccccc agcaccagca ggatgaggcg cgcgcgcagc gaacgcggta cgaagtaacg
     2101 caggaaccgc atcagggcga gaaccggtag ccgatgcccc agacggtttg gatccagcga
     2161 ggttgcttgg ggtcgtcttc gatggcgcga cgcaggcgca ggatggcgat gtcgatggcg
     2221 cggtcgttgc gctcgccgtc gtcgtcgcgc gccagggcca gcaggcgttc gcgcgacagg
     2281 ggcttgccgg cattcttgac cagggcttcg agcaggttga tctcgccgcc ggtgagcttg
     2341 acctggatgt cgtcgcgcat gagctggcgg gtggccgggt cgaacacgaa ggggccgaag
     2401 gaaacggggg cgtccttggt gagcgccgag gggccgcgct tgcggcgcag cacggcctcg
     2461 atgcgggcca gcagttcgcg cgggtcgaag ggcttgccca ggtagtcgtc ggcgccggct
     2521 tccaggccga tgatgcggtc gacggcttcg tcgcgcgcgg tcagcaggat gacggggatg
     2581 tcctcgccct gttcgcgcag ccggcggcag gcgtcggcgc cgtcgccgcc gggcagcatg
     2641 cggtcgagca ccagcaaatc gggggcatag cgggtgatgc gggcggacag gtcgctggcg
     2701 tccggggcga gcagggtgtc gtagccgtgc cggttgaggt aatcggccag caattggcgc
     2761 agggccgggt cgtcgtcgac gaccagcagc ttggtgtggg tgggatccat acctggatag
     2821 tgccgtgggt ggggggttag ggcaagggcg gggaaatcgt ctgaaaacta ttactggcct
     2881 tatggtggag agatcggggg cgaatttggg ttctttagcc gccgcggctg ccctgcggaa
     2941 ttccgatgct cgcccccact gggggctgtg cttcggattc ctccggggtc ctcggctctc
     3001 gattgggcaa atttgtcgcg ccctgtgaac ggggcacggc cggggcagta aaatcgcgcc
     3061 actatgcatt gtgtcccctt tcatgtctga cccgcgcgcc ctcgacgttc tgcggcgcgt
     3121 cttcggttac gaatccttcc gcggcgaaca gcaggccatc gtcgaccacg tcatcggcgg
     3181 cggcgacgcc ctggtgctca tgcccacggg gggcggcaag tcgctctgct accaggtgcc
     3241 ctcgctggtg cgcgagggca cgggggtggt ggtgtcgccg ctcatcgccc tgatgcagga
     3301 ccaggtcgat gcgctcaccg agctgggcgt gcgcgcggcc ttcctgaatt ccacccagga
     3361 ctggcagcag gtgcgcgagg tggagcaggc cttcgtcaat ggcgagctgg acctgctcta
     3421 cgtggcgccc gagcggctca tgaccgatcg ctgcctgcaa ttgctcgagc gcgggcgcat
     3481 tgcgctcttc gccatcgacg aggcgcactg cgtgtcgcaa tgggggcacg acttccggcc
     3541 cgaatacctg cagctgtcca tgctgcacga gcgctggccc gacgtgccgc gcatcgcgct
     3601 gacggccacg gccaccgcca tgacgcgcgc cgagatcgcg cagcgcctgg cgctggatac
     3661 cgcgcaccat ttcgtggcga gcttcgaccg gcccaatatc cgctaccgca tcgtcgagaa
     3721 gaacgaggtc cggcgccagt tgctggacct gatccgcagc gagcacgcgg gcgactcggg
     3781 cgtggtgtat ggcctgtcgc gcaaccgggt cgaggaaacg gcgaactttc tctgcgagca
     3841 tggcatcaac gccttgccct accatgcggg gctgagcgcc gcggtgcgcg cctcgaacca
     3901 gtcgcgcttc ctgcgcgaag acggcatcgt catggtggcc accatcgctt tcggcatggg
     3961 catcgacaag ccggacgtgc gcttcgtggc gcacatcgac ctgcccaagt cggtggaggg
     4021 ctattaccag gaaaccggcc gggccggacg cgacggcctg ccggccacgg cctggctggc
     4081 ctatggcctg caggacgtgg tgcagcagcg ccgcatgatc gacgagtcgc cgggcgacga
     4141 ggtgtttcgc cgccgcctgg gccagaacct ggacgccatg ctgggcctgt gcgagacggt
     4201 gcaatgccgc cgcgtgcgcc tgctggcgta tttcgggcag acgatcacgg cctgcggcaa
     4261 ttgcgatgtg tgcctggagc cgcccgaggc ctgggacggc acggtggcgg cgcagaaggt
     4321 gctgtcggcg gtgtatcgcc tgcaacagcg cggccagcgc tacggcgccg ggcatttgat
     4381 cgacatcctg cgcggcaaga gcaccgaccg cacgcggcag aacgaccacg agtcgctgag
     4441 cgtgttcggc gtgggcaagg acttgtccga gcagacctgg cgcggcgtgc tgcggcagtt
     4501 gctggcgcag ggcctgctga tggtcgacgg cgagggctac ggcacgtttg ccctgaccga
     4561 agccagccgg gaagtgctca agggcgagcg caaaatcatg ctgcgccgcg acctggagaa
     4621 aaaggaaaag gcgccgcgcg gcggcacgcg cgccagggcg ccggtggtcg acctgccggc
     4681 cgaggccgtg ccgctgttcg aaaccctgcg cgcctggcgc ggcgaggtgg ccaagaacca
     4741 cggcgtgccg gcctatgtga tcttccacga tgccacgctg cgcgagatcg ccatggcgcg
     4801 gccggcgtcg ctggacgtgc tggggcacat cagtggggtg ggcgcgcgca agctggaagc
     4861 ctatggggaa gaaatcctgc gctgtctcca ggcaggttga aagcatgtcg ccgtgcctag
     4921 gcgttcacgt cttgtttcaa tcgtaaggta tacaattttt aggagaaatc cctaaaacag
     4981 gcgggattcg gggaaatcct gggtgctcgg atgccaaata aagacctatc attggataca
     5041 gtattcaatt ccaatcgtag attccttcgc tccgaggtct ttgcccatgt tcgcttttcc
     5101 ccgcattccg cggcgcgcgc agctcgccgc acgcatctcc aggcgcctgt ccaggcaggc
     5161 ggtcgcggcg gcctgcctgg gcggcagcct gctggcggtc tgccagcctg gcctggccca
     5221 ggcggcccag cctctggtga aggtccgtta cgaagaggtg gtgcgctccg tgctctacct
     5281 gcccatgtac gtggccctgc accggaatct gttcaaggaa gcgggcctgg atgtcggcat
     5341 gaaaacctcg caaggcacgg acaagggcat ggcggccctg ctgtccggca gcgccgatat
     5401 cgtcctgatc ggccccgagg cctcggtcta cgtcgccggc agcgagtcgc cggtcaagcc
     5461 caggattttc gccggcctga ccgccaccga cggtttcctg ctgatgtcgc gcgagcccgc
     5521 gccggccggc ggtttcgact ggtccaggct caagggcaag aacatcatgt ccttccgtcc
     5581 cgggtccaat cccgatgtgt tcctggagac ggccttgcgc aagcatggcc tggacccgaa
     5641 gcaggacgtg aaactggtca acaatatcgg cccggccgcg cgcatgggcg cctggctggc
     5701 cggccagaac gactacgcca cctttctgga gcccgaggcc ggcgcgctgg aaaaagacgg
     5761 caagggctac gtgctggcct ccgtcggggc cgaggccggc ccggtggact acacggtctt
     5821 cacgacgacc gacacctata tccggcagca tcccgaggtg ctgaaggcgt ggacgcaggt
     5881 catcgcgcag gccgatcgca tcgtgcagag cgcgccggcc gatcaactgg ccgccgatgt
     5941 ggccgaattc ttccccggca tcacccagcc ggaactggct cacgccattg agcgctaccg
     6001 gacctaccac ctgtggaagg cgacgccggc ggtcactgcg caagccatcg agacgctgca
     6061 ggacatgctg gtgaccagcg gcgtgctcga gccgggcaag cgcgtgccct acgaccaact
     6121 ggtggtgggc gacttcacgg cgcaggtgca atgatgcgcg acgacgcgcg catcgaggtg
     6181 cgcaatgtct cgctgcgcta tttcacgcag cagggcgaaa ccctggcgct ggacgatatc
     6241 tcgctgtcga tcgcgccggg cgagttcgtc agcatcatcg gccagtcggg ttgcggcaag
     6301 agcacgctgc tttcgctgat cgccggcatc atgccgcctt cttccggcag cgtgcgggtc
     6361 gacggcaggc cggtggacgg cccgacgccg cgcgtcggct acatgctgca gcaggactat
     6421 ctgtatgaat ggcgcagcat cctggacaac gccctgctgg gcgcccagat ccagaagcgg
     6481 gacatgcgcc aggcccggga gcgggcggtg cgcctgctgc agaaatgcgg catgggggac
     6541 tttctgcacc attacccgcg ccagctctcc ggcggcatgc gccagcgcgt ggcgctggcc
     6601 aggacgctgg cgaccgatcc ggacgtggtg ctgctcgacg agcctttttc cgcgctggat
     6661 tcccaaaccc gcctggctat cgccgatgag gtggtggacg tgctgcgcga ggcaggcaaa
     6721 tccgtggtgc tggtgacgca cgacatcggc gaagcgattt ccatgaccga ccgggtcgtc
     6781 gtgctgtcgc gccggcctgg ccgcatcaag tcgcagcacc gcatggattt tcccagcctg
     6841 ggccgccggc ccaagccttt cgaggcacgc gcgctgcccg agttcaattc gtacttctct
     6901 tccctctggg aagagctgga cgtccatgtc gagggttgag gcgatgcgta ccgagatcca
     6961 tgctgcttcc gccgcggccg cgggccggac cgcggccagg cccgccatca gccgggacta
     7021 cgccaggtgg cagcagcggc agcgccgccg ccgcgccggc gtgctgatcg ccaggctgct
     7081 gctgctggcg ctgttcttcg gcctgtggga actgctggcg cgcatgcagt gggtcaatcc
     7141 cctgctgacc agctatccca gcgccttgtg gccgaccttt ctgcaattga tgcgggaggg
     7201 gcatctgctg gtgcacacct gggccacgct gcgcgccacg ctggtgggtt tcgtcctgag
     7261 catggtgctg ggcgtggtca ttgccgccgg gctgtggtgg tcgcccttcg ccagcaaggt
     7321 gctcgacccg tttctggtcg tggccaatgc catgcccaag atcgccttcg tgcccatttt
     7381 ctacatttgg ctggggtcgg attattcgat ctacggcatg gcggtggcca tcgcggtatt
     7441 cgtgaccatc atggtggcct acgagggctt ccaggcaatc gatcccaaca agatcaagct
     7501 ggccgtcacc ctgggggcca gccgcgccca ggtgctgcgc ctggtcgtgc tgcccggcag
     7561 cgtgccgacg ctgatctcgg cgatgaaaat gaacgtgggc ctgtcgctgg tgggcgtcat
     7621 cgtgggcgag ttccagtccg ccaatgaagg gctgggcttc ctgatcgtca acggcagcca
     7681 ggtattcaag ctcaacattg tcatgacggc cattgtgatc ctggccctgc tgtcggcgct
     7741 gatgtatttc gtcatcgccc ggctggaggc cgcattggcc aagcgatacg cctgagaaat
     7801 actggaggaa ccgtggaatt tcccgattgg gtcgtggatc gcgtggtggc gcgccaaggc
     7861 gcgctgcatc cctacccccg ctttgacgca agcagcacgg cgttcgtgat cgtcgatatg
     7921 cagcgctact ttacccagcc cggctaccag ggcgagtgcc cggcgtcccg ggataccttc
     7981 gccgccgtga accgcctggc cgccgcgttg cgggccggcg gcggcagcgt ggtctgggtg
     8041 cagaccaacg ccgatggcgc cgacgggttc tggtcgcatc accatgccgc gatgctgacg
     8101 cccgagcgca gcgcgcgccg cctgcgcgaa ctgtccgcct cgagcccggg cttcgagatc
     8161 gcgccggggc tggacgtgca gccccaggac gcgcgcgtcg tcaagcgctg ctacagcgcg
     8221 ctggtgccgg gttcctcgga cctggacgcc gtgctcaagg cgcgcggcat ccgcacggtg
     8281 ctggtgggcg gcacggtcac caatgtgtgt tgcgaatcca ccgcccgcga cgcgatgatg
     8341 ctggattacg ccaccgtgat ggtcgacgat gcgctgtcgg ccgtcaccca ggccgagcac
     8401 gtgaacgcgc tgcacaactg gatattgttc ttcggcgacg tgctgagcgc ggacgaaacg
     8461 atcgcgcggc tgggttagaa cagttcgctg ctgtcgccgg ccgtgcgtgc ggccggcggc
     8521 gtcaggccca ggtgccgcca cgtcatctgc gtggccatgc ggccgcgcgg ggtgcgctgc
     8581 aggtagccct gctggatcag gtagggctcg atcacgtcct cgatggtgtc gcgctcttcg
     8641 ccgatggcgg cggccaggct gtcgacgccc acggggccgc cgtcgaactt gtgcaccacg
     8701 gcctcgagca gcttgcggtc catcaggtcc aggccctgcg ggtcgacttc cagcatggcc
     8761 agcgcgcggc cggccacgtc gtggtcgatg gtgccgctgg ccttgacttc ggcgtagtcg
     8821 cgcacgcggc gcagcaggcg gttggcgatg
//
LOCUS       DASWPO010000025         5888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1165706, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000025 DASWPO010000000
VERSION     DASWPO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165706"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VGC69_01145"
     CDS             <1..146
                     /locus_tag="VGC69_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY0293918.1"
                     /translation="QNSQHRADAMKSWLHHYNWHRPHQGIGRAVPISRLNLDGYNLLT
                     LHT"
     gene            complement(143..802)
                     /locus_tag="VGC69_01150"
     CDS             complement(143..802)
                     /locus_tag="VGC69_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0293919.1"
                     /translation="MLTLITANKNYSSWSLRAWLALRVAGIAFKEKRLALFTDEFARQ
                     LGGVTPAGLVPVLLDGNFAIWDSLAICEYVAERHPEARLWPSDPRARARARSLAAQMH
                     SGFAQLRRVLPMNIEAHLPGIDVSDAQQDISSVQAIWHDTRAEFGRGGPFLFGQFSIT
                     DAFYAPVVSRFVTYGIAAAGPVHDYMDAILALPAMQEWTREARAEAIFVPDDEPYRTH
                     R"
     gene            complement(844..3741)
                     /gene="uvrA"
                     /locus_tag="VGC69_01155"
     CDS             complement(844..3741)
                     /gene="uvrA"
                     /locus_tag="VGC69_01155"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0293920.1"
                     /translation="MDAIRIRGARTHNLKNVSLDLPRHKLVVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMDKPDVDLIEGLSPAISIEQKAASHNPRSTVGTIT
                     EIHDYLRLLYARVGTPYCPDHGLPLQAQSVSQMVDAAQAWPAESRLAVLAPIARGKKG
                     SFEDEFASLQAQGYVRLRVDGEMVELETMPVLKKTEKHDIDVVIDRLRIRPESTQRLA
                     ESFETALQLADGRAIALDMDSGREQMFSSRYACPVCSHSLPELEPRLFSFNNPMGACP
                     TCDGIGQVGFFDPKRVVAFPELSLAAGAIRGWDRRNSFAYSLLSSLAAHYDFDIDAPF
                     EELSETVRDKVLFGSGEEELSFLYLNEKGKSTVKHHPFEGVIPNLERRWRETDSANVR
                     EELGKFRNIKTCPDCGGSRLRSEARHVLIGNDIDDKPGSAGQRLGRAIYEVEAMPLSA
                     CLGWFQALALTGAKQEIAQRIVREIEARLSFLNNVGLTYLSLDRSADTISGGEAQRIR
                     LASQIGSGLTGVMYVLDEPSIGLHQRDNDRLIATLQHLRDLGNSVIVVEHDEDMIRSA
                     DWVVDMGPGAGEHGGHVVAQGTPEDIQADVNSVTGQYLNGQRTIAVPRRRPVADDQPW
                     LTLSGATGNNLKDVTLSIPAGRLVCVTGVSGSGKSTAVNDTLSVAVARALNHAQGEPA
                     PYAALTGLEHFDKVISVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPEARTRGYDPG
                     RFSFNVKGGRCEACQGDGVVKVEMHFLPDMYVPCDVCHGKRYNRETLEIRYRGRNISE
                     VLDLTVGQAVEYFDSVPAIARKLQTLVDVGLSYIRLGQSATTLSGGEAQRVKLSQELS
                     RRSTGRTLYILDEPTTGLHFRDIELLLEVLNQLVDSGNTVLIIEHNLDVIKTADWLVD
                     MGPEGGDGGGRVVAQGTPEDVAASPDSHTGHYLARVLQPHVKMAVQSVQAK"
     gene            3907..5088
                     /locus_tag="VGC69_01160"
     CDS             3907..5088
                     /locus_tag="VGC69_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0293921.1"
                     /translation="MPASRLKLNPTERRASIALAGLFACRMLGLFLLLPVFAVAARGL
                     PGGDDPARVGLALGMYGLTQAFMQIPFGLASDRWGRRPVVVAGLLLFIIGSVVCAQGH
                     DVFWITIGRAIQGAGAISAAVTAWLADATRDEVRARAMAMVGSSIGLSFAVSLVASPL
                     LVGEWGLHGLFWVIAALGVASLGVARWVVPRVPANDSRSMQSARPAQVLKHRDLLRLN
                     FGVYVLHFIQVSLFVVAPGLLARTGDFRTNELWKVYLPIILVSFVLMVPGVIYAEKRR
                     AHRGALLCAVAGLALVCGLLPAASLHFYGLVAALTGFFVAFNILEALQPSLVSRLAPP
                     AYKGLALGFYNTAQAAGLFSGGALGGWVAAHAGAPAVFVMAAVLALAWFLVSWRLRPL
                     S"
     gene            5227..5736
                     /gene="ssb"
                     /locus_tag="VGC69_01165"
     CDS             5227..5736
                     /gene="ssb"
                     /locus_tag="VGC69_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY0293922.1"
                     /translation="MASVNKVILVGNLGRDPEVRYSPDGAAICNVSIATTSQWKDKAS
                     GERREETEWHRVVMYNRLAEIAGEYLKKGRSVYIEGRLKTRKWQDKDTGADRFSTEIV
                     ADQMQMLGGREGGGEGSFGGGGGYEESSRSSAPRAPAARPAAAPRNAAPASAPAGANL
                     ADMDDDIPF"
BASE COUNT          863 a         1968 c         2031 g         1026 t
ORIGIN      
        1 accagaactc ccaacatcga gccgatgcca tgaaatcctg gctacaccac tacaactggc
       61 atcgaccaca ccagggcatc ggccgcgctg tccccatctc cagactcaac ctcgacggat
      121 ataacctctt gactcttcac acctagcggt gcgtgcggta gggctcgtcg tccgggacga
      181 agatggcctc ggcccgggct tcgcgcgtcc attcctgcat ggcgggcagc gccaggatgg
      241 cgtccatata gtcgtgcacc gggccggcgg cggcgatgcc gtaggtcacg aagcgcgaca
      301 ccaccggcgc atagaaggcg tcggtgatgc tgaactggcc gaacaggaag ggcccgccgc
      361 ggccgaactc ggcgcgggtg tcgtgccaga tggcctgcac gctggagatg tcctgctggg
      421 cgtcgctgac gtcgatgccc ggcaggtggg cctcgatgtt catgggcagc acgcggcgca
      481 gctgggcgaa gccgctgtgc atctgcgcgg ccagcgagcg cgcccgggcg cgggcgcgcg
      541 ggtcggacgg ccagaggcgg gcttcggggt ggcgttcggc cacgtactcg cagatggcca
      601 gcgagtccca gatcgcgaaa ttgccgtcga gcagcaccgg caccaggccg gccggggtca
      661 ccccgcccag ctgccgcgcg aattcgtcgg tgaacagggc cagccgtttt tccttgaagg
      721 cgatgccggc gacgcgcagc gccagccacg cccgcagaga ccaggacgag taattcttgt
      781 tggcggtgat gagggtgagc atggctgggc ccgtgtgccg ctgcgttgcg actgataagc
      841 agtttacttg gcttggacgc tttgcacagc catcttgaca tgcggttgca atacccgggc
      901 caggtagtgc ccggtgtggc tgtccggcga ggcggccacg tcttcgggcg tgccctgggc
      961 caccacccgg ccgccgccgt cgccgccttc cggccccatg tccaccagcc agtcggccgt
     1021 cttgatgacg tccaggttgt gctcgatgat cagcacggta ttgccgctgt cgaccagctg
     1081 gttgagcact tccaggagca gctcgatgtc gcggaaatgc aggccggtgg tgggttcgtc
     1141 caggatgtac agggtgcggc ccgtgctgcg gcgcgacagc tcctgcgaga gcttcacgcg
     1201 ctgcgcctcg ccgcccgaga gcgtggtggc gctctgcccc aggcggatgt acgacaggcc
     1261 cacgtccacc agcgtctgca gcttgcgggc aatggcgggc accgaatcga agtattccac
     1321 cgcctggccc acggtcaggt cgagcacctc gctgatgttg cggccgcggt agcggatctc
     1381 cagcgtctcg cggttgtagc gcttgccgtg gcagacgtcg cagggcacgt acatatcggg
     1441 caggaaatgc atctccacct tgaccacgcc gtcgccctgg caggcctcgc agcgcccgcc
     1501 cttgacgttg aagctgaagc ggcccgggtc gtagccgcgc gtgcgcgcct cgggcacgcc
     1561 ggcgaacagt tcgcggatgg gcgtgaacag gcccgtgtag gtggccgggt tgctgcgcgg
     1621 cgtgcggccg atggggctct ggtcgacgct gatgaccttg tcgaaatgct ccagcccggt
     1681 cagcgcggca tagggggcgg gctcgccctg cgcgtggttg agcgcgcgcg ccacggccac
     1741 cgacagcgtg tcgttgaccg cggtggactt gcccgagccc gacacgcccg tcacgcacac
     1801 cagccggccg gccgggatgc tgagggtcac gtccttgagg ttgttgccgg tggcgcccga
     1861 cagcgtcagc cagggctggt cgtcggccac cggccggcgg cgcggcacgg cgatggtgcg
     1921 ctggccgttg aggtactggc cggtgaccga gttgacgtcg gcctggatgt cctcgggcgt
     1981 gccctgggcc accacgtggc cgccgtgctc gcccgcgccg gggcccatgt ccaccaccca
     2041 gtcggccgag cggatcatgt cctcgtcgtg ctcgaccacg atcacgctgt tgcccaggtc
     2101 gcgcaaatgc tggagggtgg cgatgagccg gtcgttgtcg cgctggtgca ggccgatgga
     2161 cggctcgtcg agcacgtaca tcacgcccgt caggcccgag ccgatctggc tggccaggcg
     2221 gatgcgctgc gcctcgccgc cggagatcgt atcggcgctg cgatccagcg acagatacgt
     2281 caggcccacg ttgttcagga aactgagccg cgcctcgatc tcgcgcacga tgcgctgcgc
     2341 gatctcctgc ttggcgccgg tgagcgcgag cgcctggaac cagcccaggc aggccgacag
     2401 cggcatggcc tcgacctcgt agatggcgcg gccgagccgc tggcccgcgc tgccgggctt
     2461 gtcatcgata tcgttgccga tcagcacgtg gcgcgcctcg gaacgcaggc gcgaaccgcc
     2521 gcaatcgggg caggtcttga tattgcggaa cttgcccagc tcttcgcgca cattggccga
     2581 gtcggtctcg cgccaacgcc gctccagatt gggaatcacg ccttcgaagg gatggtgctt
     2641 gacggtgctc ttgccctttt cgttcaggta cagaaaggac agttcttcct cgcccgaacc
     2701 gaacagcacc ttgtcgcgga ccgtctcgga caattcctcg aagggcgcgt cgatgtcgaa
     2761 gtcgtagtgc gccgccaggc tcgagagcag cgaataggca aagctgttgc gccggtccca
     2821 gccgcggatg gcgccggccg ccaggctcag ctcgggaaag gccaccaccc gcttcggatc
     2881 gaaaaagccc acctggccga tgccgtcgca ggtggggcag gcgcccatgg ggttgttgaa
     2941 gctgaacagg cgcggttcga gctcgggcag gctgtggctg cacaccgggc aggcgtagcg
     3001 gctggagaac atctgctcgc ggccgctgtc catgtccagg gcaatggcgc ggccgtcggc
     3061 cagctgcagg gcggtctcga agctctcggc caggcgctgc gtgctctcgg ggcggatgcg
     3121 caggcggtcg atgaccacgt cgatgtcgtg cttctcggtt ttcttgagca ccggcatggt
     3181 ctcgagctcg accatctcgc cgtcgacgcg caggcgcaca tagccctgcg cctgcaagct
     3241 ggcgaattca tcctcgaagc tgcccttctt gccgcgcgcg atcggcgcca gcacggccag
     3301 gcgcgactcg gccggccagg cctgcgccgc gtcgaccatc tggctcacgc tctgcgcctg
     3361 cagcggcagg ccgtggtcgg ggcagtaggg cgtgcccacc cgcgcgtaca agaggcgcag
     3421 gtagtcgtgg atctcggtaa tggtgccgac ggtggagcgc gggttgtggc tggccgcctt
     3481 ctgctcgatg gaaatcgccg gcgacaggcc ctcgatcagg tccacgtcgg gcttgtccat
     3541 caactgcagg aactgccgcg cgtaggccga caggctttcc acatagcggc gctgcccttc
     3601 cgcgtacaag gtgtcgaagg ccagcgacga cttgcccgag ccggagagtc ccgtcaccac
     3661 caccagcttg tggcggggca ggtctagcga tacgttcttg aggttgtggg tgcgggcacc
     3721 ccgaatgcga atcgcgtcca tcgggctcca gcggctcgtc gagccggcgt gtaagcaagg
     3781 tttcaaggct aacttgttac tatagcgcgc ccaatcggtt cggacgaaga tgcccgtccg
     3841 gtttggcgag aaattgacga tctgcacaac cagaaagccc gccctgggtc tgcttcctcc
     3901 cactgaatgc cggcttcccg cctgaaactg aatcccactg aacgccgcgc cagcatcgcc
     3961 ctggccgggt tgttcgcctg ccgcatgctg ggcctgttcc tgctgctgcc ggtgttcgcc
     4021 gtggcggccc gcggcctgcc ggggggcgac gaccccgccc gcgtcggcct ggcgctgggc
     4081 atgtacggcc tcacccaggc cttcatgcag attcccttcg gcctggcctc cgaccggtgg
     4141 gggcgccgcc cggtggtggt ggccggcctg ctgctcttca tcatcggcag cgtggtctgc
     4201 gcccaggggc acgatgtgtt ctggatcacc atcggccggg ccatccaggg cgccggcgcc
     4261 atttcggccg ccgtcaccgc ctggctggcc gacgccaccc gcgacgaagt ccgcgcccgc
     4321 gccatggcca tggtgggcag ctccatcggg ctgtcctttg ccgtgtccct ggtggcttcg
     4381 cccctgctgg tgggcgaatg gggcctgcac ggcctgttct gggtcatcgc ggccctgggc
     4441 gtcgccagcc tgggcgtggc ccgctgggtg gtgccccggg tgccggccaa cgactcgcgc
     4501 agcatgcagt ccgcccgccc cgcccaggtg ctcaagcacc gcgacctgct gcggctcaat
     4561 ttcggggtgt acgtgctgca cttcatccag gtgtcgctct tcgtggtggc gcccggcttg
     4621 ctggcccgca ccggcgattt ccgcaccaat gagctgtgga aggtctacct gcccatcatc
     4681 ctggtgtctt tcgtgctgat ggtgccgggc gtgatctatg ccgaaaagcg ccgcgcccac
     4741 cgcggcgccc tgctgtgcgc cgtggccggc ctggcgctgg tgtgcggcct cctgccggcc
     4801 gcctccttgc atttctacgg gctggtcgcg gccttgacgg gctttttcgt cgccttcaac
     4861 attctcgagg ccctgcagcc ctcgctggtg tcgcgcctgg cgccgccggc ctacaagggg
     4921 ctggccctgg gtttctacaa cacggcccag gcggcaggcc tgttctcggg cggcgcgctg
     4981 ggcggctggg tggccgcgca tgccggggcg cccgcggtgt tcgtcatggc cgcggtcctg
     5041 gcgctggcgt ggtttctggt ttcctggagg ctgcggccgc tttcctaggg cggacagcgg
     5101 cgcccctcgg ggacaggctc attgcccacc gttcgtgaca tttttgtcgc ctggcgggtt
     5161 tgtggccggc cgggtaaatc gctgataatg acccatcact gaattttcaa atataggggc
     5221 acaactatgg catccgtcaa caaagtcatc ctcgtcggca acctcggacg cgaccccgaa
     5281 gtgcgctatt cgcccgatgg cgcggccatc tgcaacgtgt ccatcgccac cacctcgcag
     5341 tggaaggaca aggcgtcggg cgagcgccgc gaagaaaccg aatggcaccg cgtggtcatg
     5401 tacaaccgcc tggccgagat cgccggcgag tacctcaaga aggggcgctc ggtgtacatc
     5461 gagggccgcc tgaagacccg caagtggcag gacaaggata cgggcgccga ccgcttcagc
     5521 accgagatcg tggctgacca gatgcagatg ctgggcgggc gcgaaggcgg cggcgagggc
     5581 agcttcggcg gcggtggcgg ctacgaggag tcgtcgcgtt cgtcggcgcc gcgcgccccg
     5641 gccgcccgtc cggcggcggc ccccaggaac gcggcgcccg cgtccgcgcc ggcgggggcc
     5701 aacctggcgg atatggacga cgatattccg ttctagaagc tacttgagct ttgcaaatta
     5761 aagggccctc ggcattcacg ccgagggctt tttgcttgga cgatcttgga aacgggtttc
     5821 gtccgtcgat cccagcggcc atcaaccaaa ggctgcggag gcccgtaccc ggaagtgatc
     5881 gcagcaca
//
LOCUS       DASWPO010000026         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2059359, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000026 DASWPO010000000
VERSION     DASWPO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059359"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1167
                     /locus_tag="VGC69_01170"
     CDS             217..1167
                     /locus_tag="VGC69_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0293923.1"
                     /translation="MSEPVYVGIDVAKASFEVAVSGKEQTLSLGNDEAGHAELCQVLI
                     PLTPRLILLEATGGHEQHLALALAAAGLRVSVINPRQARDFARCMGKLAKTDRIDAQV
                     LRGFAALLDAQGHEPRALLDEQQRELTALVTRRRQLVAMLVAERQRLALAHPKAKASI
                     LAIMDAIAAQLHDVDGQLRQHVQAHHADLAKLLTSVKGVGPTTASTLLAQLPELGHLN
                     RKQITSLVGLAPMNRDSGTLRGQRHIFGGRADVRRVLYVAALVGTRFNPVLKAFYAKL
                     LAAGKPKKVALVACMHKLLIILNAIARSKSPWSNQIAGIA"
     gene            complement(1214..1927)
                     /locus_tag="VGC69_01175"
     CDS             complement(1214..1927)
                     /locus_tag="VGC69_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0293924.1"
                     /translation="MALELGGKNALIVLGDADLDAAINGGAWASWLHQGQICMSAGRH
                     LVHQDLAAEYAERMAEKARALPVGDPHTQPVALGPIITAQQVQRVDRIVQASIAMGAR
                     LLAGGTYQGQFYAATVLADVTPEMPVFREEIFGPVVSVTRFGSDAEAIRLANDSEYGL
                     SAGVYTRDIGRGMAMAEQLHVGLVHVNDQTVNDDGTMPMGGRGASGNGSRHGGPANWD
                     EFTQWQWTTVRNAAPMYPL"
     gene            complement(1894..2313)
                     /locus_tag="VGC69_01180"
     CDS             complement(1894..2313)
                     /locus_tag="VGC69_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0293925.1"
                     /translation="MLTQPRGLLLPSAEGCTSLARRVPHGVVGVISPFNFPFLLTSRA
                     VLPALATGNAVVLKPDPRTPVSGGVLLALLLEAAGLPKNVFHMLPGDAEAGEALVTDP
                     NTAMISFTGSTAVGRRVGELAGAISRRWRSNWAARTH"
BASE COUNT          408 a          847 c          787 g          424 t
ORIGIN      
        1 tatctttcaa atacttcaaa cttcagtggt cgaagtgcat gcaagcacgt cgatcagtga
       61 acgggttgac tcgccactgc gcagcctggg tctccttcgg gcccgagccc gcttccaagc
      121 acagggcgcg cggtggtggt ctctttgttc tgctgcatcc cgaatggttg aacgaacaca
      181 tttcgtattc ataaggctgg gagccggaga actgctatga gtgaacctgt ctacgtgggc
      241 atcgatgtcg ccaaggccag cttcgaggtg gctgtcagcg gcaaagagca aaccttgagc
      301 ctgggcaacg acgaggccgg ccatgccgag ttgtgccagg tgttgatccc tctgacacca
      361 cggctgatcc tgctggaggc cacgggcggc catgagcagc acctggcgct cgcgctggcg
      421 gcggcgggcc tgcgcgtgtc ggtcatcaac ccgcgccagg cacgcgactt cgcgcgctgc
      481 atgggcaagc tggccaagac cgatcgcatc gacgcgcagg tgttgcgcgg cttcgccgct
      541 ttgctcgacg cgcagggcca cgagccgcgt gcgctgctcg atgagcagca gcgcgaactc
      601 accgcactgg tgacgcgtcg gcgccaactg gtcgccatgc tggtggccga gcgccagcgc
      661 ctggcgctgg cccacccgaa ggccaaggcc agcatcctgg cgatcatgga tgcgatcgcc
      721 gcccaactcc acgacgtgga cgggcaactg cgccagcacg tgcaggccca ccacgccgat
      781 ctggccaaac tgctgacctc cgtcaaaggc gtcggaccca cgacagccag cacgctgctg
      841 gcgcagttgc ctgaactcgg ccacctcaac cgcaagcaga tcacctcgct ggtgggcttg
      901 gccccgatga accgcgactc gggcaccttg cgcgggcagc gccacatctt cggtggccgc
      961 gccgacgtgc gtcgtgtgct gtacgtggca gccttggtgg gtacccgctt caatcccgtg
     1021 ctcaaggcct tctacgcaaa gctgctggcc gctggcaaac ccaaaaaggt cgccctcgtg
     1081 gcctgcatgc acaagctgct gatcatcctg aacgcgatcg ctcgctccaa gtcgccttgg
     1141 agcaatcaga ttgcgggcat tgcttgagtt agacattcaa gatggttgct tgggttgttt
     1201 ttcgaaatct tcttcagagg ggatacatcg gcgcggcatt gcgaaccgtc gtccattgcc
     1261 actgggtgaa ctcgtcccag ttggcgggtc cgccgtgccg cgagccgttg cccgaggcgc
     1321 ctcggccgcc catcggcatg gtgccgtcgt cgttcacggt ctggtcgttc acatgcacca
     1381 ggcccacgtg aagctgctcg gccatcgcca tgccccggcc gatgtcgcgc gtgtagacgc
     1441 ccgcggacaa gccgtattcg ctgtcgttgg ccagacggat ggcttccgca tcgctgccga
     1501 atcgcgtgac cgacacgacg gggccgaaga tctcctcgcg aaacacgggc atctccggcg
     1561 tgacgtcggc cagcacggtc gccgcataaa actggccctg gtaggtgccg ccggccagga
     1621 gcctcgcgcc catagcgatg gacgcctgca cgatgcgatc gacccgttgc acctgttgcg
     1681 cggtgatgat gggcccgagc gccaccggtt gcgtgtgcgg atcgcccacc ggcagcgccc
     1741 tggccttttc cgccatgcgc tcggcgtact cggcagccag gtcctggtgg accaggtgcc
     1801 ggccggcgct catgcagatc tggccctggt gcagccagga cgcccaggcg ccgccattga
     1861 tcgccgcatc gaggtctgcg tcgcccagga cgatcagtgc gttcttgccg cccagttcga
     1921 gcgccacctt cttgagatgg cgcccgccaa ctcgccgacc cggcggccta ccgccgtcga
     1981 acccgtgaag gaaatcatcg ccgtattcgg gtcggtcacc agcgcctcac ccgcctcggc
     2041 gtcacccggc agcatgtgga acacgttctt cggcagcccc gcggcctcca gcaggagcgc
     2101 cagcagcacg ccgccgctca ccggcgttct cgggtccggc ttgagcacca cggcgttgcc
     2161 cgtggccagc gccggcagca cggcacggct ggtgagcagg aacgggaaat tgaacggcga
     2221 gatcacgccc accacgccgt gcggcacgcg gcgcgccagg ctcgtgcagc cctcggcact
     2281 cggcagcaac agcccccggg gctgggtgag catcgccgcc gcctcatggc agtggtgcag
     2341 caccgagtcg attctcgaac atcgccttgg gtgggatgcc gccggtctcg cgaacgatcc
     2401 agttcgcgaa gtcctgccga ttggactcga gcagcgcggc cgcccggcgg aacaccttgg
     2461 cccgct
//
LOCUS       DASWPO010000027        42575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_898661, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000027 DASWPO010000000
VERSION     DASWPO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 42575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42575
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898661"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="VGC69_01185"
     CDS             <1..113
                     /locus_tag="VGC69_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY0293926.1"
                     /translation="EKKQLGDRLEQGWRLACQLWLEHDIELAQDAPGAQA"
     gene            110..457
                     /locus_tag="VGC69_01190"
     CDS             110..457
                     /locus_tag="VGC69_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY0293927.1"
                     /translation="MKPLFHGVCNTMYVILTSKPGVYRTESNADIRLIETWDYVLEGR
                     VRARFQLGELLRETRVNVVDAGETSAVNSVPSKFLERFDTLEAGRRELRQLCHFGSLD
                     AALVAVRPDEAAP"
     gene            511..810
                     /locus_tag="VGC69_01195"
     CDS             511..810
                     /locus_tag="VGC69_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0293928.1"
                     /translation="MVNITFVSNDNKQAQAPENSNLLRVSLREKGGIPFKCGGGLCGT
                     CKCTIESGREHLDAIKPKERKLLTQDQIDSGMRLACQTFVQGDIAVSWQPVAAAK"
     gene            818..1738
                     /locus_tag="VGC69_01200"
     CDS             818..1738
                     /locus_tag="VGC69_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY0293929.1"
                     /translation="MLHISDAMIDELVDVQSAQQVLREAFLYFGKGEAAMQSRERTDA
                     GGVKLSTLGAVIPGQGVTGAKVYTTIQGKFDFVILLFSTEDGRPLATLQANAITRLRT
                     AATTLVAASHLASREARRMVLYGAGVQGQAHARQFALAYPLEWIMVSDPHVDGARLRD
                     MERACGVPVLRSPLRAPIEEADIIVTASRSQTPLFEGERLQPGTFVAAIGSSLPHTRE
                     LDDNALAKADLLAVEWLAQARQEAGDLVLADARLALERRYVELGDLVAGAHPGRQSAG
                     QITLYKAVGVGLEDIALAGLAYRRLQAQGQ"
     gene            complement(1792..2463)
                     /locus_tag="VGC69_01205"
     CDS             complement(1792..2463)
                     /locus_tag="VGC69_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0293930.1"
                     /translation="MPNAAGNAPDKTAPLGISHSPLTQLVVDALRDKILNGDIAPGER
                     LVESRLSEEFSVSRMPVREALRQLAANGLVTIEPRRGATVTAFQGTQLRELVEVRATL
                     EALNAKLAAQRHDPKQIAELNALLAEGMRLANNPDDRETLALLNRRFHDALGHIAANS
                     VLQEVMRTLRDRTAVLFAPFLAQRTQQNWEEHAAILRAVINGDAELASLLAARHVYNA
                     AQMEP"
     gene            complement(2582..2827)
                     /locus_tag="VGC69_01210"
     CDS             complement(2582..2827)
                     /locus_tag="VGC69_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293931.1"
                     /translation="MTQPVLYLPSTTQGLLSSAKPILGWNALRPSGLLARVEQAFVAW
                     QTARLQVLTPERLWAPALQDTRSFAEIRRSRRLQRQG"
     gene            3191..4729
                     /locus_tag="VGC69_01215"
     CDS             3191..4729
                     /locus_tag="VGC69_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293932.1"
                     /translation="MKMELSIGPAHWAYLAGVLAIVLTMIFRANVVVPSIIATAVVAF
                     FWSGSIITAIGSIFSASMVAAKELFNIFLIIALMTALLNSLKSIGADIRMVRPFRAVM
                     TNGHNAFVILAVATYLISLFFWPTPAVPLVAAVLLPAAIAAGLPPLAGAMAIAIAGQG
                     MALSSDYMIGVAPAISAKAAGGISAALVADRALALSLIVGLVALVLSWVKIRKFIQPG
                     SPALLDRWELAPSGAQVQHVEGAASFDKSAIAQGTAHDHDLPTEAAIDAELRRDPHAR
                     ASRLFAVVTPLAFLAIILVMLAPKFGIQGAPDLKGGDGALLIGGVAALLMIWVTFSAS
                     KGRKAFEVAADHVTDGFVFAFKAMGAVLPIAGFFFVGAKGTAGGILGISGAEHVPDLL
                     FELVQAGQHLLPQNGLLVGFGVLIVGMITGIDGSGFAGLPLTGAVSGALGPVAGVDVA
                     TLAAIGQMGAVWTGGGTLIAWSSLIAVAGFSRVPVMELARALLIPVVAGLILAVVGGV
                     LIWG"
     gene            4928..5635
                     /locus_tag="VGC69_01220"
     CDS             4928..5635
                     /locus_tag="VGC69_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0293933.1"
                     /translation="MELGSTAFFIALLEIIWVNILLSGDNAVVIALAARSLPRAQQRQ
                     AIVVGSAAAIVMRIVLTLVAAQLLLLPWLKFIGALLLVYIGVSLLLPEGDDDGKARQH
                     ASLLAAIRTIMLADLVMSLDNVVAVAAAAMGNTTLLVLGLTISIPLVIFGSTLLLKVI
                     ERFSLIVWVGAALLGFIAGELMAGDPALRDLVARLDTRLGLDPSSLSLLAGAVGALLV
                     LGLGKGLLSRRKAPRNA"
     gene            5720..6436
                     /locus_tag="VGC69_01225"
     CDS             5720..6436
                     /locus_tag="VGC69_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0293934.1"
                     /translation="MLELFQTLNWAAVFQIILIDILLGGDNAVVIALACRNLEPRQRM
                     QGILWGTAGAIFLRLVLVAFALTLLTIPFLKLAGAALLLWIGVKLMQSDEEGHEVRAG
                     ASIMGAVKTIIVADFVMSLDNVIAIAGAAESGGDTHQVGLVAFGLLVSVPIIVWGSTL
                     VLKLIDRFPIVVTLGAALLGWIAGGMAIDDAFLVSRIGEQPAAVKITAEICGAVLVVL
                     LGRWMARRRAATKEASHESA"
     gene            6423..6557
                     /locus_tag="VGC69_01230"
     CDS             6423..6557
                     /locus_tag="VGC69_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293935.1"
                     /translation="MSLRKTAQPIATPQSFNSGLSLLQGLALLAVLGAVVALVVSHFA
                     "
     gene            6595..7377
                     /locus_tag="VGC69_01235"
     CDS             6595..7377
                     /locus_tag="VGC69_01235"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0293936.1"
                     /translation="MRPIAILTRPAGRNEALADRLSTAGWQVLALPALVLDPLPDAGP
                     PPLPHDFDLVVFVSGNAARLYLEQAGLPGGWPAGTLAATVGPASAQALRDSPCFGPQG
                     RILHPPAEAATHDSEALWHLLSSGPLPRRVLLVRGEAGRDWLADRLAEAGAQVERYAL
                     YRRRAACWPPASVLQLRRMAADPAPQAPVWLLTSMAGIDAVAASVRAAGLFDWWLRGR
                     FVVTHPRLGKHLLAVAESAGQTAVVKSCMPADEAIFEALAAA"
     gene            7425..8597
                     /locus_tag="VGC69_01240"
     CDS             7425..8597
                     /locus_tag="VGC69_01240"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY0293937.1"
                     /translation="MTDTSSDLSPPAPMDAPGTGAAASGAKPTARTQRSGGGLLTAFI
                     ILLVVAVALAAALWAQRRQFLAAGREVATQIEQLNRDLAQARQTGGQALALAQSQTNQ
                     VADLEARIRDVQTQNSALQQAWQSFSSGASDEMLLNDVEHSVNLANQQLRLAGDAGNA
                     IVALETAQSRLAQADRPRLAGLQQSINGDLDRLRAVSTVDVPAQSARIERLMTLVGQA
                     PLLSPDSAAQATAPQETAAPASPAAQAVASLPADASWWMRWRAELATWPARAGATMVH
                     EFGDLIRVQRVAQPDAILLSAEQAGMVRAALRQRLMTAQLALMMRQQPVWKTEIDNVQ
                     DTLAHYYDTHAPDTMSAIELAHQLAQTEVTVRLPDVADSLSTLAALRADGAKSGQD"
     gene            8683..10284
                     /locus_tag="VGC69_01245"
     CDS             8683..10284
                     /locus_tag="VGC69_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEY0293938.1"
                     /translation="MRTWLWTLLLAVVAVALAVVLRQHPGNVLLMITPWRIELSLTLA
                     VLLLLALFVVLYVVLRLVGWLLAIPERMRAWRGRRAQARDQELIEQGWIGLLEGRYVH
                     AEKDFAKLHGQTRSATRRVLAALSAARAAHHMGEFARRDQLLDASREDAGIDHRLHEA
                     TASVAADLLLDQGQAQRALDVLAPLQDGGARHLHTLRLLLRAEKALDHHERVFTLARS
                     LLRRRAIEKSEALQLIDQAGAARLRAGMANGDGWRGIWKEIKTEERVLPNIALAGAAA
                     FDGAGEGVDAGRILEAAIVDKFNPALVTAYAHCQADQVPRRLARAETWLQQRPTDANL
                     LTALGLLCLNGQLWGQAERYLQRSLSRRDDPQCHALLGRLYDRLNRPEDAMRHWRLAT
                     EARIVLPVLASDAVLPPAETSADPDPYRVDAGGTYAPPPADTPAGAAAESAASEPMPL
                     SAPPAVDYVIEPEARERVERAKPIAEPAAQVPPRSSVDIEDYFDSAPIPAAAFEAPVS
                     QAPEAGATAPSDAVPGRNPQTEQKG"
     gene            10288..10821
                     /gene="ppa"
                     /locus_tag="VGC69_01250"
     CDS             10288..10821
                     /gene="ppa"
                     /locus_tag="VGC69_01250"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY0293939.1"
                     /translation="MSLDRVPSGKSLPDDFNVVVEIPMNADPVKYEVDKESGAVFVDR
                     FMLTAMHYPCNYGYIPQTLSEDGDPADVLVISPFPIAVGVVVRCRAIGVLEMDDESGG
                     DAKLLAVPVSKLYPVYDCIKSYEDLPAQDINRIQHFFEHYKDLEKGKWVKVKGWMGLE
                     AAREEILRSAERYKKKA"
     gene            10931..11626
                     /locus_tag="VGC69_01255"
     CDS             10931..11626
                     /locus_tag="VGC69_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0293940.1"
                     /translation="MDYVVTPTPSAALPVAGSKALFPVHRVYCVGRNYVEHAKEMGFT
                     GREDPFFFIKPNDALVAIADGETGSLAYPLKTSNLHYEMELVVALGKGGTNLSVEQAA
                     ECVWGYALGLDMTRRDLQGEAKKLGRPWEVGKAFDHSGPIGPIHPRSVTGTLDKGAIW
                     LDVNGERKQSSSLDKLIWNVAESIAYLSGLFELAAGDLIYTGTPEGVGAVVKGDLIVG
                     GVDGLGELRLKII"
     gene            11669..11809
                     /locus_tag="VGC69_01260"
     CDS             11669..11809
                     /locus_tag="VGC69_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEY0293941.1"
                     /translation="MIRKRVLFATLALAAVVLAGCHTMAGAGKDLQGAGSAITNSAEK
                     HE"
     gene            complement(11905..13230)
                     /locus_tag="VGC69_01265"
     CDS             complement(11905..13230)
                     /locus_tag="VGC69_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0293942.1"
                     /translation="MNAKSAPSPAMLRKVLISSVLGNAFEWFDFAIYGMFAVVIAKLF
                     FPTGDATASLMLTLATFGMGFVVRPIGGVLLGLYGDRVGRKKALTLTIVLMAIGTGLI
                     GILPTYAAIGVAAPVLIVLARMLQGLSAGGEFSSATTMLIEFAPRHRRGLYGSLQMCS
                     QALAFSLGAAVAYLITSSLTPEHLMSWGWRLPFLVGILVGPVGWLIRSRVDESPEFAS
                     YLDQAEHNKKARPRAPLAEVFRDYPRAMFASIGVCVTGTVSAYVFVFFVPLFSRQQFG
                     ISLADANLATFAGTLTILIFCPLAGWLSDKVGRKAVLVPAIVGYAIVGLFCFRYLVAS
                     PSFQSLLLLQVCVSFFMSFFWGPTPVVMTEIFPVDIRATGSAFTYNLSVLLFGGLAPF
                     INTWLVQATGSKFAPLYYVLFSALLGVVGALLLPGRQAAAQARPAQSPA"
     gene            complement(13267..14424)
                     /locus_tag="VGC69_01270"
     CDS             complement(13267..14424)
                     /locus_tag="VGC69_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY0293943.1"
                     /translation="MQQETYLGALTQEMRGWRRDIHQHPELGFQERRTSGLVAGLLKS
                     WGIEVTEGVGRTGVVGTLRGAGGPGMALGLRADMDALPMDELGTAAHRSVNAGVFHGC
                     GHDGHTAIMLGVAKSLATGKRDFRGVVHFIFQPAEETLGGGSAMLEDGLFERFPCDEI
                     YALHNISLLPSGTVGIRTGAILAASNGFRIRIQGVGTHAAMPHKGTDPILVGSALVQA
                     LQSIVSRNVNPLDTAVVSVCQFNAGTARNVIADHAELEGTLRTLSTEVQKLTLKRVQE
                     ICDAAAAMYGCGIALEWLAGAPPTMNAPEQADVVRRAVVNTLGADKLDPDMAPLMAAE
                     DFAYMLQARPGAYFFLGHDGQMCHHPQFDFDDETSPLGVSVFLDIVKQRLG"
     gene            14550..15050
                     /locus_tag="VGC69_01275"
     CDS             14550..15050
                     /locus_tag="VGC69_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0293944.1"
                     /translation="MQEGRPKPPGRENEGARFLTLKLDLIKTEMISRANSAYRELCGL
                     DVRQLRVLRNICSRPGVTASDVVRQSLIEKTLLSKMLAELIQRKLVRRTVHPADARHF
                     QLWPTPAGLHVRDQADVLGGRLEDDMLAPLTPAERESLNQLVNKLMAMFLDDSPRGGP
                     ADGKRA"
     gene            complement(15047..15685)
                     /locus_tag="VGC69_01280"
     CDS             complement(15047..15685)
                     /locus_tag="VGC69_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY0293945.1"
                     /translation="MSELQTAADQLAEIGPAVSLFGSARIPPDSPYYAKTLAIAAALA
                     KAGFAVIAGGGPGIMEAANKGAFEAGGCSVGLNIKLPHETTDNVYQTHSLHFHYFTAR
                     KTTFFMHSMAYVALPGGFGTLDELFEALTLLQTNKLPATPIILVGGEFWGGLIDWMRG
                     HMLSAGMISPADLSLFTIEDDPDRIVGQLVSFYGQYLEAAGKKPPSLPTTLG"
     gene            15738..18524
                     /gene="polA"
                     /locus_tag="VGC69_01285"
     CDS             15738..18524
                     /gene="polA"
                     /locus_tag="VGC69_01285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY0293946.1"
                     /translation="MNKTLLLVDGSSYLYRAFHAMPDLRNAQGEPTGAIYGVANMLRR
                     LVQDHKADYAACIFDARGKTFRDDLYPEYKSHRPPMPPDLAAQIEPIHRLVRALGWPV
                     LAVEGVEADDIIGTLARKAAEQGVDTIVSTGDKDLAQLVDSHVTLVNTMSGEVLDEAG
                     VSAKFGVPPERIVDYLMLVGDAVDNVPGVTKVGPKTAVKWLSEYGSIEQLVAAADGIK
                     GVAGENLRAAIAQFGLTRELLTVKCDCDLAGHVESLQDLAFGEANAPTLTEMYDRYGF
                     RTWLREQTGDAQRVPLGDARIARVPAGAPGDPDEPGAPPVAPVATDYRIVTDWAAFDA
                     WMDLVKPAALVALDTETTSLEEMQARLVGVSMAVAPGVACYIPLAHRGPDAASGLDLD
                     DSAVTQLPKDEVLARLRTWFEDASAAKLLHHAKYDTHVLANEGIALAGIAEDTMLQAY
                     VLESHRGVGLSDLAKRYLGRSGVTYEELCGKGAKQIGFDEVAISKAGFYSAEDADFTL
                     QLHQVLRPRLAAEAGLETIYKLEMQVSHVLTVIERNGVKVDAEELGRQSHVLGQEMLV
                     QEQRAFELAGQPFNLNSPKQLGEILFERMGLPVVRKTPGGAPSTDEEVLTKLAQDYPL
                     PQVLLDYRGLSKLKSTYTDKLPRMIDPATGRVHTHYSQAAVITGRLASSDPNLQNIPV
                     RTEAGRRVREAFIAERGVLLSSDYSQIELRIMAHVSNDANLQSAFAAGEDIHRATASE
                     VFGVPLADVTGEQRRAAKAINFGLIYGMGVFGLASSLGITRDAAQAYIGRYFDRYPGV
                     ARYMDDIKQLAHAQGYVETVFGRRLYFPDINGGSGPRRQGAERAAINAPMQGTAADLI
                     KMAMVQVQQWLDEEKLQSRMIMQVHDELVLEVPDDEVGLVREQLPRLMCGVAELRVPL
                     VAEVGVGKNWEQAH"
     gene            18654..20816
                     /locus_tag="VGC69_01290"
     CDS             18654..20816
                     /locus_tag="VGC69_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0293947.1"
                     /translation="MRNLPTLIDSSSDTAVMIADARGIVTYVNVGFTRLFGYQRDEAM
                     GQLLGTLLVGANTDVNTLYRLQHADDVGQKVADNVILLYPKTGRPLWAAMSINHDYDA
                     QGEVDNTLVMLADITTSKMYEVLQHAILDALAHEQPLGEVMTLLCKEVENLAPEVVST
                     VVRVDERQCLRPLASPSLPESYADHLDGAAIGVGRASCGTAAWLGESVTTYDIATDEN
                     WDGFRDLVLPLGLRACWSNPIKSHDGRVLGSFAFYFREPRAPDEFHRRLIDSCLHLCA
                     LAFEREEARSNIRRLAFYDQLTGLANRNTLRVEAGGMLERVRRQGGLLALLFIDLDRF
                     KTINDTQGHAMGNLVLNEVALRLGSNVRISDLVARAGGDEFVAVLPDCSIEQVARVAD
                     KMLQALNQPIDLGNGVSVITGASIGAALYPDDGDDVDTLMLHADMAMYQAKHDGRACL
                     RFFHHDMKDRTRERAALETALGESLRAGSLCLHYQPKINHLGMVYGVEALARWDHPLL
                     GKIPASRFIPLAEESHLINFLSEWAIGEACRQMAVWQSEGIAVPHMAVNVSALDFKNR
                     DLAGVLRSALTRNGLQPAQLVLEMTEGVMFDDAPDTMAMIQSVRDLGVHLSLDDFGTG
                     YSSLSYLHRLSVNEIKIDRSFVCDLVPGSKSQALVRTMLNIGRSLGLTVVAEGVETEA
                     QRDFLLLNGCLVMQGYLYSHPMSPQDFTRWMQGRVVPA"
     gene            20904..21728
                     /locus_tag="VGC69_01295"
     CDS             20904..21728
                     /locus_tag="VGC69_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY0293948.1"
                     /translation="MLLLWIFLATILGGLIAIGIAQWLAYRVFAHYLHHMVSLSVGVL
                     LSVALLHLLPEAFEGSDTNPRTMFAVLLAALLGFFVLEKISVLRHSHHHEGDGHHHHH
                     GHDSHEAGKGGRLILVGSSLHNLCDGVLVAAAFLTDPLLGVLTAASIIVHEVPHKLGD
                     FVVLINAGLARRTAVLLILFSSLCTAVGGVAGYFVLHEVQAYTPYLLVAAAGSFLYIS
                     VADLIPQMHERVHLSDAIPQVLLVGVGVAIIYGVTTFMEGVEHHDAAEAAVAHAHK"
     gene            21784..22575
                     /locus_tag="VGC69_01300"
     CDS             21784..22575
                     /locus_tag="VGC69_01300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0293949.1"
                     /translation="MSTAPAAGPVAATVIHTDTQGLAQGDFRLAVQGGAIDAYYAAPE
                     GKKDLPVVLVVQEIFGIHEHIKDLCRRLGKAGYLAVAANLYERQGDASAYTDIPKLVS
                     ELVSKVSDEQVLSDLDASLAWAGQNGGDTKRVGVTGFCWGGRLAWMYAAHNPAVKAGV
                     AWYGKLTTGHGPLIKTMAVDIADKIHGPMLGLYAENDASIPLDSVEEMKKRLAAAGNA
                     AAKASEIVVYPGAHHGFLADYRPMYDKHAAEDGWKRMLGWFGKYL"
     gene            22599..23990
                     /locus_tag="VGC69_01305"
     CDS             22599..23990
                     /locus_tag="VGC69_01305"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY0293950.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAALLFVQGLARDGLLARTA
                     QVRVELYGSLGATGKGHGTDKGVMLGLMGQAPDTVDPAAIPGLLQAVRETRSLVLSGG
                     RAVPFVEKEHVVFYRREAMAEHPNGMKFHAYDGDGQALRESRYLSVGGGFVVTAGTTN
                     SQVIAAHDSLPFPFRTARELLQMCRASGLTVAGLMLENELAWRSREDVEAGLDRIWAV
                     MQDCVSRGCGIGQPDMVCELPGPLRVRRRAPELYRRLTRQAERSLSDPLAVMDWVNLY
                     AMAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYDRFVPGASQAGVREFLLTAGAIGLL
                     YKYNASISGAEVGCQGEVGVACSMAAGALAAVLGADVEQVENAAEIGMEHNLGLTCDP
                     VGGLVQIPCIERNAMASVKAVNASRMALRGDGQHYVSLDSVIKTMRETGADMKTKYKE
                     TARGGLAVNIVEC"
     gene            complement(24023..24745)
                     /locus_tag="VGC69_01310"
     CDS             complement(24023..24745)
                     /locus_tag="VGC69_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0293951.1"
                     /translation="MPRKSSIPSLADQNAASGGVAAVDRALSLLDAFSLTAPLLTLAE
                     IAHRTQLHKSTVLRMLASLEHAHLVQKREDGRYALGAGIERLHRIHALSFSLGSVVMP
                     VLGDLVARTQESAAFHVRQGDQRLCLHRVDSPRPVRDHLAVGALLPMDRGAGGRVLSA
                     WPDANDPISQRIRREFVVTLVGDRLPELAGVASPVFGAGGELVGALTLTMPAERLESG
                     FDRIVLAHARKLTALLGGSYPQ"
     gene            24862..25839
                     /locus_tag="VGC69_01315"
     CDS             24862..25839
                     /locus_tag="VGC69_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0293952.1"
                     /translation="MIRRAFLAALACAALPLASVPSLAAPDYPDAPVRFIVPFPPGGG
                     TDNVSRVLAHGIAEDTGWNLVIENHAGAGGNIGISLVAKGKPNGLIIGMGQTSNLAIN
                     PTLYATVPYDAARDFTPVALVATQPTVLMVRAESPFHTLQDVIAAAKAKTGSLTIANS
                     GVGTVSHLSAVLLAKVAGVKLLDVPYAGASPAITSLAGGQVDLSMSAPSSVAPLVKAG
                     KLRALAVTSAQRLPILPEVPTVAESGYPGFEALDWKAVVAPAGTPADVVAALNAAVNK
                     ALTRQDVINALLADGSTVAGGTPERFGQFLASEQKRWGDTVRESGARVD"
     gene            25872..27071
                     /locus_tag="VGC69_01320"
     CDS             25872..27071
                     /locus_tag="VGC69_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0293953.1"
                     /translation="MTKPLRHVRVLDLTNVLAGPFCCHQLAHMGAEVIKVETPGTGDL
                     ARQLGADAELNRALMGVSFLAQNAGKRSITVNLKHPRGKEVLLRLVESADVLVENFRP
                     GVMQRLGLGFEVLRQRQPRLVYCAISGFGQDGPLHDLPAYDQIIQGMSGAMSVTGDAG
                     SAPLRVGYPVADTAGGLTAAFAVAAALAEPNRETARYIDVSMLESVMTTMGWAISNYL
                     VAGREPRPMGNENVTASPSGTFRTGDGLLNIAANKQEQFEALCRVVGRPGLIDDPRYR
                     ERQARLQHRYELKAEIEAALAADSAEHWWPRLTEAGVPTGPVYSVPQALAHPQVRDRG
                     LIGQFDDAPGVGRAVRVVRAGYKVDGRTPSVDTPPPTLGQHTDALLRELGYGEDEIRA
                     LREEQAL"
     gene            27068..27928
                     /locus_tag="VGC69_01325"
     CDS             27068..27928
                     /locus_tag="VGC69_01325"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEY0293954.1"
                     /translation="MSETASPSPGAAEVENASSAWWRTGVIDMQPGVIRYRGYAIEDL
                     IGNVGFAQMIWLMLRGDLPTPGQGALLEAALMSAVDHGPQAPSIAIARMAATCGVGLN
                     NAMASAVNVLGDVHGGAGEQAVALYRDVAARLDAGLPLAEAARAALRDYVELHGKFVS
                     GFGHRFHPLDPRSPRLLQLVDDAAARGEVSGRYAAIARAIEAELAAQKSRPIPMNIDG
                     ATAVIYAELDFPAPLARGLFCLSRSVGILAHAWEQTQQGGRNKGPIPRQYLPQYEGVP
                     PRELPDWAKQ"
     gene            complement(27977..28651)
                     /locus_tag="VGC69_01330"
     CDS             complement(27977..28651)
                     /locus_tag="VGC69_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEY0293955.1"
                     /translation="MTDVVRPQLALPEGRQKVLLHSCCAPCSGEVMEAMTASGIDYAI
                     FFYNPNIHPVREYEIRKNENIRFAEQHDIPFIDADYDVDNWFERVKGLEDSPERGERC
                     TKCFDMRFERTALYAHEHGFDTITSSLGISRWKDMNQINGSGARAAARYGDLVYWTYN
                     WRKGGGSQRMIEISKRENFYQQEYCGCVYSLRDSNRHRRAQGRERIEIGVKFYGKEGL
                     VNEESL"
     gene            complement(28747..29025)
                     /locus_tag="VGC69_01335"
     CDS             complement(28747..29025)
                     /locus_tag="VGC69_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0293956.1"
                     /translation="MAQATARHILVSTEAQANELKTAIQNGASFADLAKQHSSCPSSQ
                     AGGDLGSFGPGEMVPEFDKVVFSAPVNEVQGPVKTQFGYHLVEVTSRQ"
     gene            complement(29109..29582)
                     /locus_tag="VGC69_01340"
     CDS             complement(29109..29582)
                     /locus_tag="VGC69_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0293957.1"
                     /translation="MRSARNVEAIASALIELTGCLNSPRQDEVLLSVAGVSLDRALFP
                     LLVRLHAAGTTGVAQLAEQVGRDPSTVSRQLAKLEQLGFVCRPAGQNDKRVRAASVTG
                     VGVRTLARITDARRKLLGELIKDWSAEERRIFPDLVQRLANSMKKKQSGTDMKSP"
     gene            29649..30269
                     /locus_tag="VGC69_01345"
     CDS             29649..30269
                     /locus_tag="VGC69_01345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0293958.1"
                     /translation="MDRAKTVLLIMGSTRAARICPKITAWIAEIGQTCTGLAYETVDL
                     ADWLLPMDDEAALPALGPYAQPHTRAWSDKIKSADAVVFVAPQYNWGYPAVLKNAIDH
                     LYHEWRDKPAAIVTYGGHGGTRCAKQLRQVATAVKMRMVMKAPAIRLSEAVIRQGASF
                     IPERDFLPYRPQIQHALVELAAQVAGRERFLTRVGRKWKAFLFAMT"
     gene            complement(30352..31407)
                     /locus_tag="VGC69_01350"
     CDS             complement(30352..31407)
                     /locus_tag="VGC69_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0293959.1"
                     /translation="MRFSDFDQRLAALGAQPVHRGRVVRVWLNGQALDSGTRRRRSEH
                     FLPRALRDAVPALTAELDGLARIRSEHAGNDGSRLLVELADGQMVESVLLPRDGLCVS
                     TQVGCAVGCRFCMTGKSGLIRQVSSMEILAQVVLARRLRAVKKVVFMGMGEPAHNLDN
                     VLEAIDLLGTEGNIGHKNLVLSTVGDRRVFEALPRQRVKPALALSLHTTKAELREHLL
                     PRAPKIAPEELIELGEKYARDADYPIQYQWTLLKGVNDGDDELDAILRLLKGKYGVLN
                     LIPFNSLEGDDYQRPDPERIREIVHALHSRGVLTKVRNSAGQDVDGGCGQLRARAIGA
                     AQGVELRRQRTAKPEAA"
     gene            complement(31451..32218)
                     /gene="map"
                     /locus_tag="VGC69_01355"
     CDS             complement(31451..32218)
                     /gene="map"
                     /locus_tag="VGC69_01355"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY0293960.1"
                     /translation="MTKRPEEIALLAESGRLLADVFGYVDRLGLIGMTTMQVNNLVDS
                     LIVHELNARPASKGQYGYAYALNASRNNVVCHGVPSTTDLLQSGDIVNFDITLEKNGY
                     IADSSKTYLVGEVPPPAKRLVQVAYEAMWQGIKAVRPGARLGDVGHAIERHARRNGYS
                     VVREYCGHGIGRDMHEPPQVLHWGKPQTGLVLREGMVFTIEPMINQGRDAVRTEEDGW
                     TVVTRDGQLSAQFEHTVAVTRNGVRVLTLRPEEKMPN"
     gene            complement(32215..32427)
                     /locus_tag="VGC69_01360"
     CDS             complement(32215..32427)
                     /locus_tag="VGC69_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HEY0293961.1"
                     /translation="MGIVNIDDDLHDQLRKASAVSCRSINAQAAFWIRIGRLCEMNPT
                     QSFNEIMARELRAAGVVARAVDVAAT"
     gene            32661..33926
                     /locus_tag="VGC69_01365"
     CDS             32661..33926
                     /locus_tag="VGC69_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0293962.1"
                     /translation="MTTQAFSIACGIDFGTSNSAVGWHRPGLPSLLALEEGKPTIPSA
                     IFFHDEDAAVSYGRAALADYLAGYDGRLMRSMKNLLGSSLIDGHTEIRGRGVPFRVLL
                     THFIQELRTRAQQAAGREFTSVVMGRPTFFVDGDEVADRAAQDTLGEIAAAVGFTHIE
                     FQYEPLAAAFDYESQISREELVLVIDIGGGTSDFSLIRLGPERAAMADRRADILAHGG
                     VHIGGGDFDQYLSLDSFMPLLGLGSQLRSGKDVPSTQYVNLASWHTINFAYTNKALEH
                     LSYIRANAAERDKIDLLLRLVKERAGHWLALQVEKTKIDLSGAPSTQIGLERIDRDRV
                     LAVSRDDLDRAVAPLIDRIETTVATLLRDAGVPLDTIDTLFFTGGSSRVPLLRQRISA
                     LLPGARSVEGDRFGSIGTGLALDAARKFG"
     gene            34053..35243
                     /locus_tag="VGC69_01370"
     CDS             34053..35243
                     /locus_tag="VGC69_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0293963.1"
                     /translation="MHAHSAAHRGASPLRAWLAVGAVTLASFAFVTTEFLPVGLLPAI
                     AQEVGVQPGTAGLMVTIPGLLAAFAAPGMMLGAGRIDRRRILLVLSVLLVASNMISAF
                     AGSFPVMLLGRALLGASLGGFWTLALAAAGRLVRPQDRARATAAIMAGVTCATVIGVP
                     LGTFIAGLASWRVSFMATGALAAVSLVAQWVLVPSLPSSAALRVADLLELWRRPHSRK
                     SLLMVVLVFAAHFSSYTYIAPFLLTRAGFSLQSITWVLLGFGLIGFVSNAVASATASR
                     HLKATLLTIVTLLMLALLALPWLQGAAPAVTAAVLAWGIAFGAIPLCMSLWMHAAAPD
                     LPEAGSATFIATIQVAIAVGSSVGGAIVDRVGIPVDFRFGCVLAVLGLAMVASFGARP
                     KAAA"
     gene            complement(35282..38056)
                     /gene="glnE"
                     /locus_tag="VGC69_01375"
     CDS             complement(35282..38056)
                     /gene="glnE"
                     /locus_tag="VGC69_01375"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY0293964.1"
                     /translation="MPSSDLLAPALAWSGYLRRRIMARPDLPAWLAEQAQQPVTPAVL
                     SAWLAELAAGRATAAALPGDLCRQALRRLRERVFTLLTVRDLAGLADLEEVVGAMTAL
                     ADLAVATAYRSAARELAETHGIPRDPATGAPQEMLIVGMGKLGGGELNVSSDIDLVML
                     YGEEGETDGPRPISHHEFYGRLTRRMMPMLSELDADGQVFRTDLRLRPDGDAGPLAWS
                     LEAFEHYLIGQGREWERYAWLKARLIEARAWPDSQPEAQAQHFENLRRPFVYRKYFDF
                     DALAALRALRERIRQDWNRRALARNGLDTANNIKLGDGGIREIEFVVQLSQMIRGGRM
                     PALQCRGLIAALHAERDAGLIAADDADKLEAAYRFLRRTEHALQYREDEQTHLLPGEP
                     ERRAELAAALGFAPEAFEHTLAAHRAVVSEAFRNAFHAAGMGDLDDQVRANTGRPVED
                     ASTSDQNLAGQIRAQFGDQADELLRRTEALLASYRMQSLPETSRRRVDALLPAALRAA
                     SRTRAPSVSAIGLLDLIEAIAQRSAYLALLAEYPDTLARVARITAASPWAAQYLRQHP
                     LLLDSLIDWHTLLAPPDWPQIGRQLAADLDASLLPDGTPDVERQMNLMRDMQRQASFQ
                     LLAQDLEGALTVEQLGDQLSALADLLLAETLRRVWLLVNREPDAVPRFTVIAYGKLGG
                     KELGYISDLDLVFLFDDPRDDAAEVYAKLGRRMSSWLSTLTSSGRLYDVDLRLRPDGE
                     AGLLAVSVAAFEQYQRQHAWTWEHQALTRARYAAGDVAIGERFEHIRRDILAMPRDMD
                     KLRREVLAMRGKISAGHPNPSGLFDLKHDRGGMVDVEFVTQYLVLSQSAMHPELMENL
                     GNIALLQIAGEAGLIPAALAQHAGNAYRTLRRVQHELRLQGVERARVPAQSVAVERET
                     VKELWRVVLG"
     gene            38157..41795
                     /locus_tag="VGC69_01380"
     CDS             38157..41795
                     /locus_tag="VGC69_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEY0293965.1"
                     /translation="MTTKVLRCLFWGVLAVFAVALAACLGLRYLVLPKIDQWRPRIEQ
                     YASQSLGSPVRIGSIAADWSGLNPRLRLSSVQFYDGADQPEPTITLPSVAAVVAWRSV
                     LRMTPRLLSLDIEGADLTVRRDAAGLLWVAGMSFDPAAPAAKGDSPVLVWLSQQRQLS
                     LSGTTLHWLDEQRQAPELVLSHVDAAVRNGALSHRFALHATPPAALSAGVTLRGEIER
                     RIFTLNSSDPASWNGNFYMQLDDAEPYAWSPWVTMPAVQGRLAARAWVQLTHGQIGNV
                     TLDAVLRGLDTALPHQEGRLAAKQAHLRLDGMPGDLDALPADALPLPLARSASGQGMS
                     LQVQAQGLAATLPTVFDTPDLRVDDLQVDASLLHPPRQPLSLDLRQLQLKNGEAELQL
                     QGRWRDEGAGGEGTADLQGKLVRMAMPAIHRYLPLTVSQSTRHWMAQGLQAGEVRDAS
                     VVLKGPLAEFPFTQPGETGQFRVAGRYQEARVDYAPARPDRKGWPVLSAMSGPFVVDR
                     ASLTLDSAGGVVETGPSQQVVLHAVHAAIPNMEHDAQLDVTGESSGAVPAYLAMATNS
                     PLGGLLDGALDQSQGSGDWQVAMKLHVPLLHPDDAQIDGHIVFQGNTFQLLPQLPAMS
                     ALQGDLAFSDLGLQANDIRGTFLGGPARVSGKLGHGESLAFSGTLAATALDELGKLPA
                     WQRFSGKTAYQGKLSYAKGGDVDVSVQSDLSGLAIDLPAPLGKTASATLPLALQWSPA
                     TNRGHSGRRWLSGGIGENINLLFERDPSDRGAYFARGAIGVGRPATLPTRGLNLAGTL
                     DTLDTDAWNDALKSFSPAPVSAPVPAPAKGKAAPKPAPPALPGLELVNLSVKRLVAAG
                     QDFDDITLYAQRPAPQQWRVSIDAKQAAGTLEWAEASDAASGKITARFDRLALGKAGE
                     AGKDVKPDGDKESELSDIPGIDLQAKQFVLYGHDVGSLDLVGTNLERGNLWRLDKLHV
                     ANESATLDATGSWRLSGTERGLSADASIDFKDLGAMLERLGLQSVSSGHGTIKGKLFW
                     RNLPWRHDKADIEGTMHVNLEKGRFVHLSSRTSRVLELLSLQSLQRLATLSANPAGLL
                     REGFPFDSVTGDMRLTGGVMSIDGYKVNGPVAAIGLEGRTDIIKELWDLHAVVVPNLD
                     ASGAAIAAALVNPVIGLGAFITQWLLKQPLARAMTSEYHVTGSWDDPKVETVETKAPA
                     VESAPATH"
     gene            complement(41850..>42575)
                     /gene="rho"
                     /locus_tag="VGC69_01385"
     CDS             complement(41850..>42575)
                     /gene="rho"
                     /locus_tag="VGC69_01385"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY0293966.1"
                     /translation="ASPKSGKTVIMQHIAHAITTNFPDAVMIVLLVDERPEEVTEMQR
                     TVRGEVVASTFDEPATRHVQVAEMVIEKAKRLVEMKKDVIILLDSITRLARAYNTVVP
                     ASGKVLTGGVDANALQRPKRFFGAARNLEEGGSLTILGTALVETGSRMDEVIYEEFKG
                     TGNSEVHLERRLAEKRVYPAINLNKSGTRREELLIKPELLQKVWVLRKFIHDMDEIQS
                     MEFILDKMKATKTNSEFFDMMKK"
BASE COUNT         6696 a        14172 c        14568 g         7139 t
ORIGIN      
        1 aagaaaaaaa gcagctgggc gatcgattgg agcagggctg gcggctggcc tgccagttat
       61 ggctggagca cgacatcgag ctggcgcagg acgccccggg cgcccaggcg tgaaacccct
      121 cttccatgga gtctgcaaca ccatgtacgt catcctgacc agcaagccgg gcgtctatcg
      181 caccgaatcc aacgccgaca tccgcttgat cgaaacctgg gactatgtgc tggaggggcg
      241 cgtgcgcgcg cgcttccaat tgggcgagct gctgcgggaa acccgggtga acgtcgtcga
      301 cgcgggcgag acgtccgcgg tcaactcggt gccgtccaag ttcctggaac ggttcgatac
      361 gctggaggcc gggcgccgcg aattgcggca gctatgccat ttcggcagcc tggatgccgc
      421 gctggtcgcg gtgcgtccgg acgaggccgc gccttgacgc cgcacattgg ctccacgcat
      481 tgaatccagg aatcggacgc aggaataggc atggtcaaca ttactttcgt cagcaacgac
      541 aacaaacagg cacaggcgcc cgagaacagc aacctgctgc gtgtatcgtt gcgggaaaag
      601 ggcggcatcc ccttcaagtg cggcggcggg ctgtgcggca cttgcaagtg caccatcgaa
      661 agcggccgcg aacacctcga tgccatcaag cccaaggagc gcaagctgct gacgcaggac
      721 cagatcgaca gcggcatgcg gctggcgtgc cagactttcg tccagggcga tatcgccgtg
      781 tcgtggcaac ccgtggccgc ggcgaagtga ggatcttatg ctgcatatca gcgatgcgat
      841 gatcgatgaa ctggtcgacg tgcaaagcgc ccagcaggtg ctgcgcgagg ccttcctgta
      901 tttcggcaag ggcgaagcgg ccatgcagtc gcgcgagcgc accgacgcag gcggcgtgaa
      961 gctgtccacc ctgggcgcgg tgattcccgg ccagggcgtc accggggcca aggtctacac
     1021 caccatccag ggcaagttcg atttcgtcat cctgctgttt tccaccgagg acgggcgccc
     1081 cttggccacg ctgcaggcca atgccatcac caggctgcgc accgcggcga ccacgctggt
     1141 cgccgccagc cacctggcga gccgggaagc gcgccgcatg gtgctgtacg gcgcgggcgt
     1201 gcaggggcag gcgcatgcgc ggcagttcgc gctggcttat ccgctggagt ggatcatggt
     1261 gtccgacccg catgtcgatg gcgcgcgcct gcgcgacatg gagcgcgcct gcggcgtgcc
     1321 ggtattgcgc agcccgctgc gcgcgccgat cgaagaggcg gacatcatcg tgacggcctc
     1381 gcgttcgcag acgcccctgt tcgagggcga gcgcctgcag cccggcacct tcgtggcggc
     1441 catcggctcc agcctgcccc atacccggga actggacgac aatgcgctgg cgaaggccga
     1501 cctgctggcg gtggagtggc tggcccaggc gcgccaggag gccggcgacc tggtcctggc
     1561 ggacgccagg ttggcgctgg agcgccgcta cgtggaattg ggcgacctgg tggcgggggc
     1621 gcacccgggg cggcagagcg ccggccagat caccttgtac aaggccgtgg gcgtgggact
     1681 ggaggacatc gccctggccg ggctggccta tcgccggctg caggcgcaag gccaataaaa
     1741 gcgcccaggc cccgccatgg aaaaaccgcc gcgatcgcgg cggtttttcg ttcaaggctc
     1801 catctgagcg gcgttgtaga cgtgccgcgc ggccagcagg ctggcgagtt ccgcatcgcc
     1861 gttgatgacg gcgcgcagga tggcggcgtg ctcttcccag ttttgctgcg tgcgctgggc
     1921 aagaaagggc gcgaacagca cggcggtccg gtcgcgcagc gtgcgcatga cttcctgcag
     1981 cacggagttg gcggcgatgt ggcccagcgc gtcatggaag cgccggttga gcagggccag
     2041 cgtttcccgg tcgtcggggt tgttggccag gcgcatgcct tcggccagca gggcgttcag
     2101 ttcggcgatc tgtttcgggt cgtggcgctg cgcggccagc ttggcgttca gggcctccag
     2161 cgtggcgcgc acttcgacca gttcgcgcaa ctgcgtgccc tggaaagcgg tgaccgtcgc
     2221 gccacgccgc ggctcgatgg tgaccaggcc gttcgcggcc agttggcgca gggcttcgcg
     2281 cacgggcatg cgggagacgg agaactcttc cgagaggcgg ctttccacca ggcgctcgcc
     2341 gggggcgata tcgccgttga ggatcttgtc gcgcagggcg tccaccacca actgcgtcag
     2401 gggggagtgg ctgatgccaa gcggggcggt tttgtcaggg gcgttcccgg cggcgttcgg
     2461 catggaggtt gtctgtgtag ttattgctcg ggtcacagac gattgtatac gacacctggc
     2521 cccaattatt gggggccgga tgcctatgcc aggcgagcgt ccagggtgct ttcccggggg
     2581 ctcaaccctg ccgctgcagg cgccgcgacc ggcggatctc ggcgaacgag cgggtgtcct
     2641 gcagggcggg ggcccatagc cgctccggcg tcagcacttg caggcgggcg gtttgccagg
     2701 cgacaaaggc ttgctcgacg cgggcaagga ggccgctcgg gcgcagcgcg ttccagccga
     2761 ggatgggctt ggcggaagaa agcaggcctt gcgtggtgga gggcaggtaa agaacgggtt
     2821 gggtcatgat ggtctctctc gtgatgtgtt gtgtttggac atcacagacc tattgtatac
     2881 cgctgaccct agggtttcta cctatttttt ggttatttgc ctggatggaa atagaaattt
     2941 gccctgtttt gatgcaataa cgacaagaaa tagcggaaag aacacccgct ggacggggct
     3001 tatgcacctt atccgggatt ttatataccg aaataggtct gatccaggga gacatgcaag
     3061 aaaggagact gaaagagtcc aataattcgc atggttccag gggtaaacac cagcgtgacc
     3121 gtttttatgt atgggatatt gtatacaagg tggacgccat tcttccttta ttccttccat
     3181 cctggagcct ataaaaatgg agctttcaat cgggcccgcg cactgggcat atcttgccgg
     3241 tgtgctcgcc atcgttctta cgatgatctt ccgggccaat gtcgtcgtgc cgtccatcat
     3301 cgccacggcg gtcgtcgcgt tcttctggtc cggcagcatc attaccgcca tcggcagcat
     3361 tttcagcgcc agcatggtgg ccgccaagga actgttcaac attttcctca tcattgcgct
     3421 gatgaccgcg ctgctcaatt cgctcaagag catcggggcc gatatccgca tggtgcggcc
     3481 tttccgggcc gtgatgacca acggccacaa cgccttcgtc atcctggccg tggcgaccta
     3541 cctgatctcg ctgtttttct ggccgacgcc cgccgtgccg ctggtcgccg ccgtgctgct
     3601 gcccgcggcc atcgcggccg gcctgccgcc gctggcgggc gccatggcga tcgccattgc
     3661 cggccagggc atggcgctgt cgtccgacta catgatcggc gtggcgccgg ccatcagcgc
     3721 caaggcggcg ggcggcatca gcgccgcgct ggtggccgac cgggctctcg cgctgtcgtt
     3781 gatcgtgggg ctggtcgcgc tggtcctgtc gtgggtgaaa atccgcaagt tcatccagcc
     3841 cggcagcccg gcgctgctgg accgctggga gctggcgcct tccggcgcgc aggtgcagca
     3901 tgtcgagggc gccgccagct tcgacaagtc ggccatcgcc cagggcacgg cgcacgacca
     3961 tgacctgccg accgaggcgg ccatcgatgc cgaattgcgc cgcgacccgc atgccagggc
     4021 atcccggctg ttcgccgtgg tcacgcccct ggctttcctg gccatcatcc tggtgatgct
     4081 ggcgccgaag ttcggcatcc agggcgcgcc cgacctcaag gggggcgacg gcgcgttgct
     4141 gatcggcggc gtggccgcgc tgctgatgat ctgggtgacc ttttccgcct ccaagggccg
     4201 caaggccttc gaagtggcgg ccgatcatgt gaccgatggc ttcgtgttcg ccttcaaggc
     4261 catgggcgcg gtgctgccca tcgccggctt ctttttcgtg ggcgccaagg gcacggcggg
     4321 cggcattctc ggcatttccg gggcggagca cgtgccggac ctgctgttcg agctggtgca
     4381 ggccggccag cacttgctgc cgcagaacgg cctgctggtc ggcttcggcg tgctcatcgt
     4441 gggcatgatc accggcatcg acggttcggg tttcgccggc ctgccgctga cgggggccgt
     4501 atccggcgcc ctgggccccg tggcgggcgt ggatgtcgcc accctggcgg ccatcggcca
     4561 gatgggggcg gtgtggacgg gcggcggcac gctgatcgcc tggtcctcgc tgatcgccgt
     4621 ggcggggttc agccgggtgc ctgtcatgga gctggcgcgc gccttgctca tacccgtcgt
     4681 ggcggggctg atcctggccg tcgtgggcgg cgtgctgatc tggggctgac cggccgaacc
     4741 ggggtttgaa caagccggcg ctggtgacag cgccgatttc gttcctggcg ggacctggcc
     4801 gaagccgggt cccgcgccgg ccctgaagta tttctgacag caaattgaaa atataatgac
     4861 aggtatcgcg gcctggctgg cccgatcctg acgggccgat ccggccccca tcccgacctg
     4921 gaaaaacatg gaactcggct cgacggcctt cttcatcgcc ttactggaaa tcatctgggt
     4981 caatatcctc ctgtcgggcg acaacgcggt ggtgatcgcg ctggccgccc ggtccttgcc
     5041 cagggcgcag cagcgccagg ccatcgtcgt ggggtcggcg gcggccatcg tcatgcgcat
     5101 cgtcctgacc ctcgtggccg cccaactgct gctgctgccc tggctcaagt tcatcggcgc
     5161 gctgctgctg gtctacatcg gcgtgtcgct gctgctgccc gagggcgacg atgacggcaa
     5221 ggccaggcag catgcgtcgc tgctggcggc catccgcacc atcatgctcg ccgatctcgt
     5281 catgagcctg gacaacgtgg tggccgtcgc cgcggccgcc atgggcaaca ccacgctcct
     5341 ggtgctgggc ctgaccatca gcatccccct ggtgatcttc ggcagcacgc tgctgctcaa
     5401 ggtcatcgaa cgcttttccc tcattgtctg ggtgggcgcg gctctcctgg ggttcatcgc
     5461 gggcgagctg atggcgggcg atccggcctt gcgggacctg gtcgcgcgcc tcgatacccg
     5521 gctgggcctg gacccgtcct ccctgagctt gctggccggc gccgtgggcg cgctgctggt
     5581 gctgggcctg gggaaggggc tgctgtcgcg ccggaaagcg ccccggaacg cctgatccgg
     5641 tgctgtcgcg ccaacagttt tggtttctga cttattgctg aattacaatg cggcttttaa
     5701 cagtccagga gactgaacgg tgctcgagct tttccagacg ctgaattggg cggcggtttt
     5761 ccagatcatc ctgatcgaca tcctgctggg gggcgacaac gcggtggtca tcgcgctggc
     5821 ctgccgcaat ctggaaccca ggcagcgcat gcagggaatc ctgtggggga cggccggcgc
     5881 cattttcctg cgcctggtgc tggtggcctt tgcgctcacc ttgctgacca tcccgttcct
     5941 caagctggcc ggcgcggcct tgctgctctg gataggcgtc aagctcatgc agtccgacga
     6001 ggaggggcac gaggtgcgcg cgggcgcctc gatcatgggc gcggtcaaga ccatcatcgt
     6061 cgccgacttc gtcatgagcc tggacaacgt catcgccatt gccggcgctg ccgagagcgg
     6121 cggggatacg caccaggtcg gcctggtggc cttcggcttg ctggtcagcg tgcccatcat
     6181 tgtctggggc agcaccctgg tgctcaagct catcgaccgc tttcccatcg tggtgacgct
     6241 gggcgccgcg ctgctgggct ggatcgcggg cggcatggcg atcgacgacg cctttctggt
     6301 ctcccgaatt ggcgaacagc ctgcggcggt taaaatcact gcagaaatct gcggcgccgt
     6361 gctggtcgtc ctcctgggac gttggatggc acgccgccgc gccgccacca aggaagcctc
     6421 tcatgagtct gcgtaagact gcccaaccca tcgccactcc ccagtcgttc aactcgggcc
     6481 tgagcctgct gcagggcctg gccctgctgg ccgtgctggg cgccgtggtt gccctggtcg
     6541 tttcgcactt cgcctgagcg ggcccttgcc ggcctttctc tccatggcgc aggcatgcgg
     6601 cccattgcca tcctgacccg gccggcgggc cgcaacgagg cgctggccga caggttgtcc
     6661 acggcaggct ggcaggtgct ggcgctgccg gcgctggtgc tcgacccctt gcccgatgcc
     6721 gggccgccgc ccttgccgca cgacttcgac ctggtcgttt tcgtgagcgg caatgccgcc
     6781 cggctgtatc tcgaacaggc cggcctgccc ggcggctggc cggccggcac gctggcggcc
     6841 acggtggggc cggccagcgc ccaggccttg cgcgactccc cctgtttcgg gccgcaaggg
     6901 cgcattctgc acccgcccgc cgaggcggcc acccacgatt ccgaagccct gtggcacctc
     6961 ttgtcgagcg ggccgttgcc gcgccgggtg ctgctggtgc gcggcgaggc ggggcgcgac
     7021 tggctggccg accggctggc cgaggcgggc gcgcaggtgg agcgatatgc cctgtaccgc
     7081 cggcgggccg cctgctggcc gccggcatcc gtgctgcaac tgcgccggat ggccgccgat
     7141 cccgcaccgc aagcgcccgt ctggctgctg accagtatgg ccggcatcga cgccgtggcg
     7201 gcgtccgtcc gggcggccgg tttgttcgac tggtggctgc ggggccgctt tgtcgtcacc
     7261 catccccgcc tgggcaaaca tttgcttgcg gttgcggaaa gcgccgggca gactgcggtg
     7321 gtaaaaagct gcatgcctgc cgatgaggcg attttcgagg ccctcgctgc cgcctgaatc
     7381 gtcggggcgc ttccacgtac aatcgccacg tacaatcgcg tcatatgaca gacacatctt
     7441 ccgatctttc cccgccggcg ccgatggacg ctcctggtac cggggcggcg gcctccggcg
     7501 caaagccaac tgcgcggacg cagcgttcgg gcggcggcct gctgaccgcc ttcatcatcc
     7561 tgctcgtggt ggcggtggcc ctggcggccg ccttgtgggc ccagcgccgc caattcctgg
     7621 ccgccggcag ggaagtcgcc acccagatcg aacagctcaa ccgcgacctg gcgcaggcgc
     7681 gccagacggg cggacaggcg ctggcgctgg cccagtcgca aaccaatcaa gtggccgacc
     7741 tggaagcccg cattcgcgac gtgcagaccc agaacagcgc cctgcagcag gcctggcaaa
     7801 gcttttcgag cggcgccagc gatgagatgc tgctcaacga cgtcgagcac agcgtcaacc
     7861 tggccaacca gcaactgcgc ctggccggcg atgcgggcaa tgccatcgtg gcgctcgaaa
     7921 ccgcccaatc gcgcctggcc caggccgacc ggccgcgcct ggccggcctg cagcaaagca
     7981 tcaatggcga cctcgaccgc ctgcgcgccg tcagcacggt cgatgtgccg gcccagtccg
     8041 cgcgcatcga acgcctgatg acgctcgtcg gccaggcgcc tctgctgtcg cccgacagcg
     8101 ccgcgcaggc aaccgcgccg caggaaacgg cggcgcccgc atcgcccgcc gcgcaggcag
     8161 tggccagcct gcccgccgat gcgtcgtggt ggatgcgctg gcgcgccgaa ctggccacct
     8221 ggccggcccg tgccggggcg accatggtgc atgagttcgg tgatctcatc cgcgtgcagc
     8281 gcgtggccca gccggatgcc atcctgctgt cggccgaaca ggccggcatg gtgcgcgccg
     8341 ccctgcgcca gcgcctgatg acggcccaac tggccttgat gatgcgccag cagcctgtct
     8401 ggaagaccga gatcgacaat gtccaggaca ccctggcgca ttactacgac acgcatgcgc
     8461 cggacaccat gtcggccatc gaactggcgc atcaactggc gcaaaccgaa gtcaccgtgc
     8521 gcctgcccga tgttgccgac agcctgagca cgctcgcggc cctgcgggcc gacggcgcga
     8581 agagcgggca ggactgaaaa tatgaaacga ctcccacgcc cgctttgttc gctgtccacc
     8641 cagggggctt cggcctcctt cgggcggccg cgcggaggct gaatgcgtac ctggctttgg
     8701 accttgctgc tggcggtcgt cgcggtcgcc ttggccgtgg tgctgcgcca gcatcccggc
     8761 aacgtgctgc tgatgatcac gccgtggcgc atcgagctgt cgctcacgct ggcggtgctg
     8821 ctgctgctgg ccttgttcgt ggtgctgtac gtggtgctgc gtttggtggg ctggctgctg
     8881 gccattcccg agcgcatgcg ggcctggcgc ggccgccgcg cccaggcccg cgaccaggag
     8941 ctcatcgagc agggctggat aggcctgctc gaaggccgct atgtgcatgc cgagaaggat
     9001 ttcgccaagc tccacgggca gacccgttcg gcgacccgcc gcgtgctggc cgccttgtcg
     9061 gcggcccgcg cggcgcacca tatgggcgag ttcgcccgcc gcgaccagct gctcgatgcc
     9121 tcgcgcgagg acgccggcat cgatcatcgc ctgcacgagg ccacggcctc ggtggcggcc
     9181 gacctgctgc tcgaccaggg ccaggcccag cgggcgctcg acgttctcgc ccccctgcag
     9241 gacggcggtg cccgccacct gcacacgctg cgcctgctgc tgcgcgccga aaaggcgctg
     9301 gaccaccacg agcgggtctt caccctggcg cgcagcctgc tgcgccgccg cgccatcgaa
     9361 aaatccgagg ccctgcaact gatcgatcag gccggcgcgg cgcgcttgcg cgccggcatg
     9421 gccaacggcg acggctggcg cggcatctgg aaggaaatca agaccgagga gcgcgtgctg
     9481 cccaatatcg cgctggctgg cgccgcggcg ttcgacgggg cgggcgaggg cgtcgatgcc
     9541 ggccgcatcc tcgaggccgc catcgtcgac aagttcaatc ccgcgctggt gacggcctac
     9601 gcgcactgcc aggccgacca ggtgccgcgc cgcctggccc gcgccgagac ctggctgcag
     9661 cagcgtccga ccgatgccaa tctgctgacg gccctgggcc tgctgtgcct gaacggccag
     9721 ctctgggggc aggccgagcg ctatctgcag cgcagcctgt cgcgccgcga cgatccgcag
     9781 tgccatgcgc tgctgggccg cctgtacgac cgcctcaacc gccccgagga cgccatgcgc
     9841 cattggcgcc tggcaaccga ggcgcgcatc gtcttgccgg tgctggcgag cgatgccgtg
     9901 ctgccgccgg ccgagaccag cgccgacccc gatccctatc gggtggatgc cggcggcacg
     9961 tatgccccgc cgcccgccga cacgccggca ggcgcggcgg ccgaatccgc cgcgtccgag
    10021 cccatgcctc tgtcggcccc gcccgcggtc gactacgtga tagaacccga ggcgcgcgag
    10081 cgggtcgagc gcgccaagcc catcgcggaa cccgcggccc aggtgccgcc gcgctcctcg
    10141 gtcgacatcg aagactattt cgacagcgcg cccatcccgg ccgccgcttt cgaagccccc
    10201 gtatcccagg cccccgaggc cggcgcgacg gcgccgtccg acgcggtccc gggtcgcaat
    10261 ccccagactg agcaaaaagg ttgaaccatg agtcttgatc gtgtcccttc gggcaagagc
    10321 ctgccggacg atttcaatgt tgtcgtcgaa atccccatga acgccgaccc cgtcaagtat
    10381 gaagtcgaca aggagtcggg cgccgtcttc gtcgaccgct tcatgctgac ggccatgcac
    10441 tatccgtgca actacggcta catcccgcag acgctttccg aggacggcga tccggccgac
    10501 gtgctggtca tctccccgtt ccccatcgcg gtgggcgtgg tggtgcgctg ccgcgccatc
    10561 ggcgtgctcg aaatggacga cgagtcgggc ggcgacgcca agctgctggc cgtgccggtg
    10621 agcaagctct accccgtcta cgattgcatc aagtcgtacg aagacctgcc cgcccaggac
    10681 atcaaccgca tccagcactt cttcgagcac tacaaggacc tggagaaggg caagtgggtc
    10741 aaggtcaagg gctggatggg gctggaagcc gcccgcgagg aaatcctgcg cagcgccgag
    10801 cgctacaaga agaaggcgta aagcggcaag acagcctggg gcgatacact cccaggcgca
    10861 ttgattccaa gcccagccca aggccggatg caagaccggc cccatgcata caacttagag
    10921 gttctgccaa atggattacg tcgttactcc taccccgtcc gccgcattgc ccgtggcggg
    10981 cagcaaggcg ctcttcccgg tgcaccgcgt gtattgcgtg ggccgcaact acgtcgagca
    11041 tgccaaggaa atgggcttca ccggccgtga agacccgttc ttcttcatca agcccaacga
    11101 tgccctggtg gccatcgccg acggtgaaac gggctcgctg gcctatccgt tgaagacgtc
    11161 caacctgcac tacgagatgg aactcgtggt ggcgctgggc aagggcggca ccaacctgtc
    11221 ggtggagcag gcggccgaat gcgtctgggg ctacgccctg ggcctggaca tgacccgccg
    11281 cgacctgcag ggcgaggcca agaagctggg ccgtccctgg gaagtgggca aggctttcga
    11341 ccactccggt cccatcggcc ccatccatcc caggagcgtc accggcaccc tcgacaaggg
    11401 cgcgatctgg ctggacgtca atggcgagcg caagcaatcc agcagcctcg acaagctgat
    11461 ctggaacgtg gccgaaagca ttgcctacct gtcgggcctg ttcgagctgg cggcgggcga
    11521 cctgatctac actggcaccc ccgaaggcgt cggcgccgtc gtcaagggcg acctgatcgt
    11581 gggcggcgtc gacggcttgg gcgagcttcg cctgaagatc atctgatagc aaggtttcta
    11641 cgtccaacct caagcaaggg agcttgttat gattcgcaaa cgcgttctgt tcgcaacctt
    11701 ggccctggcc gccgttgtgc tggctggctg ccataccatg gccggcgccg gcaaggatct
    11761 gcaaggtgcc ggcagcgcca ttaccaactc ggccgagaag cacgagtaac cggctgcgct
    11821 ggagacatga aaaacgccac cttcgtggcg tttttcattt ctgcctggtt cacgcagcgg
    11881 ggcgctgcct cgacgcgcgc cggttcaggc aggcgattgg gcgggcctgg cctgggcggc
    11941 ggcttgtctg ccgggcagca gcagcgcgcc gacgacgccc agcagcgcgc tgaacagcac
    12001 gtagtagagc ggggcgaact tgctgcccgt ggcctgcacc agccaggtgt tgatgaaggg
    12061 ggccaggccg ccgaacagca gcaccgacag gttgtaggtg aaggccgagc ccgtggcgcg
    12121 gatgtcgacc gggaatatct cggtcatcac gaccggggtg gggccccaga agaagctcat
    12181 gaagaacgac acgcacacct gcagcagcag cagcgactgg aacgacggcg acgcgacgag
    12241 gtagcggaag cagaacaggc cgacgatggc gtagccgacg atggccggga cgagcacggc
    12301 cttgcggccg accttgtccg acagccagcc ggccagcggg cagaagatca ggatggtcag
    12361 cgtgccggcg aacgtggcca ggttggcgtc ggccagcgaa atgccgaatt gctggcgcga
    12421 gaagagcggc acgaagaaca cgaacacata ggccgacacg gtgcccgtca cgcagacgcc
    12481 gatgctggcg aacatggcgc gcgggtagtc gcggaacacc tcggccagcg gcgcgcgcgg
    12541 gcgggccttc ttgttgtgct cggcctggtc gaggtaggag gcgaattcgg gcgattcgtc
    12601 gacccgcgag cggatcagcc agcccacggg gccgaccagg atgccgacca ggaagggcag
    12661 gcgccagccc caggacatga ggtgctcggg cgtgaggctg gacgtgatca ggtaggccac
    12721 cgcggcgccc agcgagaagg ccagcgcctg cgagcacatc tgcaggctgc cgtacaggcc
    12781 gcgccggtgc cgcggggcga actcgatcag catggtggtc gcgctgctga attcgccgcc
    12841 ggccgacagg ccctggagca tgcgcgccag cacgatcagg acgggcgcgg ccacgccgat
    12901 ggcggcatag gtgggcagga tgccgatcag gccggtgcct atcgccatca gcacgatggt
    12961 gagcgtcagg gccttcttgc ggccgacgcg gtcgccgtac aggcccagca gcacgccgcc
    13021 gatggggcgc accacgaagc ccatgccgaa ggtggccagc gtcagcatca gcgaggccgt
    13081 ggcgtcgccg gtcgggaaga acagcttggc gatgaccacg gcgaacatgc cgtagatggc
    13141 gaagtcgaac cactcgaaag cattgccgag cacggaggag atcagcactt tgcgcagcat
    13201 ggccggcgat ggcgcggatt tggcgttcat ggggtttccc gggtcgatgg tggatggagg
    13261 aaagcgtcag cccagccgct gcttgacgat gtccaggaag acggacacgc ccagcggcga
    13321 tgtttcatcg tcgaaatcga attgcggatg gtggcacatc tggccgtcgt ggccgaggaa
    13381 gaaataggcg cccggcctgg cctgcagcat gtaggcgaag tcttccgcgg ccatgagcgg
    13441 ggccatgtcg gggtcgagct tgtcggctcc cagggtgttg acgacggccc ggcgcaccac
    13501 gtcggcctgc tcgggcgcgt tcatggtggg cggggcgccg gccagccatt cgagcgcgat
    13561 gccgcaacca tacatggccg cggcggcgtc gcagatttcc tgcacgcgct tgagcgtgag
    13621 cttctgcacc tcggtcgaca gcgtgcgcag ggtgccctcg agttcggcgt ggtcggcgat
    13681 gacgttgcgg gccgtgccgg cgttgaattg gcagacgctg accaccgcgg tgtcgagcgg
    13741 gttgacgttg cggctgacga tgctttgcag cgcctgcacc agcgccgagc ccacgaggat
    13801 ggggtccgtg cccttgtgcg gcatggcggc atgggtgccc acgccctgga tgcggatgcg
    13861 aaagccgttg ctggccgcca ggatcgcgcc ggtgcggatg cccaccgtgc cggacggcag
    13921 caggctgata ttgtgcagcg cgtagatttc gtcgcagggg aagcgctcga acaggccgtc
    13981 ctcgagcatg gccgagccgc cgcccagggt ttcctccgcc ggctggaaaa tgaaatggac
    14041 gacgccgcgg aagtcgcgct tgccggtggc cagcgacttg gccacgccca gcatgatggc
    14101 ggtgtgcccg tcgtggccgc agccgtggaa gacgccggca ttgaccgagc ggtgggcggc
    14161 cgtgcccagc tcgtccatgg gcagggcgtc catgtcggcg cgcaggccca gcgccatgcc
    14221 cggaccgccg gcgccgcgca gcgtgccgac cacgccggtc cggcccacgc cttccgtgac
    14281 ctcgatgccc caggatttca gcagtccggc caccaggccg gacgtgcggc gctcctggaa
    14341 gccgagttcg ggatgctggt gtatgtcgcg ccgccagcca cgcatttcct gcgtgagggc
    14401 gccaagatag gtttcttgct gcatggtttg atctggatgg ataagtgagc cgtgacgcgc
    14461 gcggcttccg gttgtcggcc acatggcgta aattagtagc gtaattcaat agttgaatat
    14521 ttcaactaat ggtttttacg gagtgctcca tgcaggaagg caggcccaag ccccccggcc
    14581 gcgagaacga aggcgccaga tttctgactc tcaagctgga cctgatcaag accgagatga
    14641 tcagccgggc caattcggcc taccgggagc tttgcggcct ggacgtgcgc cagttgcggg
    14701 tgttgcgcaa tatctgcagc cggccgggcg tgacggcctc ggacgtggtg cggcagtccc
    14761 tgatcgagaa gaccctgctg tcgaaaatgc tggccgagct gatccagcgc aagctggtgc
    14821 ggcgcacggt gcatcccgcc gatgcgcgcc acttccagct ctggcccacg ccggccggcc
    14881 tgcatgtccg cgaccaggcc gacgtgctgg gcggccggct ggaggacgac atgctggcgc
    14941 ccctgacgcc ggccgagcgc gagtcgctca accagctcgt gaacaagctg atggcgatgt
    15001 tcctggacga ctccccgcgc ggcggcccgg ccgacgggaa gcgcgcctag cccagggtgg
    15061 tgggcaggct cggcggcttt ttcccggcgg cttccaggta ctggccgtag aacgaaacca
    15121 gttgcccgac gatgcgatcc gggtcgtcct cgatggtgaa cagcgacagg tcggcgggac
    15181 tgatcatgcc cgccgacagc atgtgcccgc gcatccagtc gatcaggccg ccccagaact
    15241 ccccgcccac caggatgatg ggcgtggccg gcagcttgtt ggtctgcagc agggtcaggg
    15301 cctcgaagag ctcgtcgagc gtgccgaaac cgccgggcag ggccacgtag gccatgctgt
    15361 gcatgaagaa ggtggtcttg cgcgcggtga aataatggaa gtgcaggctg tgggtctggt
    15421 agacgttgtc ggtggtctcg tggggcagct tgatgttcaa ccctacactg cagcctcccg
    15481 cctcgaaggc gcccttgttg gccgcttcca tgatgccggg accgccgccg gcgatgacgg
    15541 cgaagccggc cttggccagc gcggccgcaa tcgccagcgt tttggcgtaa tagggcgagt
    15601 cgggggggat gcgggcgctg ccgaacaggc tgacggcggg gccgatctcg gccaactggt
    15661 cagcggccgt ttgcaactct gacataatca ccgggatctg ctccctggcg gggagctgcg
    15721 gattgacatt tctggccatg aataaaacct tattgctggt ggacggctcc agctatctgt
    15781 accgtgcttt ccatgccatg cctgatctgc gcaatgcgca gggcgagccc acgggggcga
    15841 tctatggcgt ggcaaacatg ctgcgcaggc tggttcaaga tcataaagca gactacgcgg
    15901 cctgcatttt cgacgcccgc ggcaaaacct tccgcgacga cctgtatccc gaatacaaat
    15961 cgcatcgccc gcccatgccg cccgacctgg ccgcgcagat cgagcccatc catcgcctgg
    16021 tgcgcgcgct gggctggccg gtgctggccg tcgaaggggt cgaggccgac gacatcatcg
    16081 gcacgctggc ccgcaaggcg gccgagcaag gcgtcgacac catcgtctcc accggcgaca
    16141 aggacctggc ccaactggtc gacagccacg tcaccctggt caacaccatg agcggcgaag
    16201 tgctcgacga ggccggcgtg tcggccaagt tcggcgtgcc gcccgaacgc atcgtcgatt
    16261 acctgatgct ggtgggcgac gccgtggaca atgtgccggg cgtgaccaag gtcggcccca
    16321 agacggcggt caaatggttg tccgagtatg gctccatcga gcaactggtg gccgcggccg
    16381 acggcatcaa gggcgtggcg ggcgagaacc tgcgcgcggc gatcgcccag ttcggcctca
    16441 cccgcgagct gctcaccgtc aagtgcgatt gcgacctggc cggccacgtc gagagcctgc
    16501 aggacctggc cttcggcgag gccaatgcgc cgacgctgac cgagatgtat gaccgctatg
    16561 gtttccgcac ctggttgcgc gagcagaccg gcgatgcgca gcgcgtgccg ctgggcgatg
    16621 cgcgcatcgc gcgggtgccg gcaggcgcgc cgggcgatcc ggacgaaccg ggcgcgccgc
    16681 ccgtggcgcc ggtcgctacc gattaccgca tcgtcaccga ctgggctgcc ttcgacgcct
    16741 ggatggacct ggtcaagccg gcggcgctgg tggcgctgga caccgagacc acgtcgctcg
    16801 aggaaatgca ggcgcggctg gtgggcgtgt ccatggccgt ggcgccgggc gtggcctgct
    16861 atatcccgct ggcgcatcgc ggccccgatg ctgcctccgg cctcgacctg gacgactcgg
    16921 ccgtcaccca gttgcccaag gacgaggtgc tggcgcggct gcgcacctgg ttcgaggacg
    16981 cctcggcggc caagctgctg catcacgcca agtacgacac gcacgtgctg gccaacgaag
    17041 gcatcgcgct ggccggcatc gccgaggaca ccatgctgca ggcctatgtg ctggaatcgc
    17101 accgcggcgt cggcctgtcc gacctggcca agcgctacct gggccgcagc ggcgtcacct
    17161 atgaagaact gtgcggcaag ggcgccaagc agatcggctt cgacgaagtg gccatttcca
    17221 aggccggctt ttattcggcc gaagacgccg acttcacctt gcagctgcac caggtgctgc
    17281 gcccgcgcct ggcggccgaa gccgggctgg aaaccatcta caagctggag atgcaggtct
    17341 cgcacgtgct gaccgtgatc gagcgcaatg gcgtcaaggt cgacgccgag gagctcggcc
    17401 gccagagcca cgtgctgggc caggaaatgc tggtgcagga gcagcgcgct tttgagctgg
    17461 cgggccagcc cttcaacctc aactcgccca agcagctggg cgagatcctc ttcgagcgca
    17521 tgggcctgcc ggtggtgcgc aagaccccgg gcggcgcgcc gtccaccgac gaagaagtgc
    17581 tgaccaagct ggcgcaggac taccccttgc cgcaggtgct gctggactac cgcggcctgt
    17641 ccaagctcaa gtccacctac accgacaagc tgccgcgcat gatcgatccg gccacgggcc
    17701 gggtgcacac gcactattcg caggccgcgg tgatcacggg gcgcctggcc tcgtccgatc
    17761 ccaacctgca gaacatcccc gtgcgcaccg aggcgggccg gcgcgtgcgc gaggccttca
    17821 tcgccgagcg cggcgtgctg ctgtcgtccg actattcgca aatcgagctg cgcatcatgg
    17881 cgcacgtgtc caacgacgcc aacctgcaaa gcgccttcgc cgccggcgag gacatccacc
    17941 gcgccacggc gtccgaggtg ttcggcgtac cattggccga tgtcacgggc gagcagcgcc
    18001 gcgcggccaa ggccatcaac ttcggcctga tctacggcat gggcgtgttc ggcctggcgt
    18061 ccagcctggg catcacgcgc gacgcggccc aggcctatat cggccgctac tttgaccgct
    18121 acccgggcgt ggcgcgctat atggacgaca tcaagcagct ggcgcacgcc cagggctacg
    18181 tggaaacggt cttcggccgg cgcctgtatt ttcccgacat caacggcggc tccggcccgc
    18241 gccgccaggg cgccgagcgc gccgccatca acgcgcccat gcagggcacg gcggccgatc
    18301 tcatcaagat ggccatggtg caagtgcagc agtggctgga cgaggaaaag ctgcagtcgc
    18361 gcatgatcat gcaggtgcac gacgaactgg tcctggaagt gcccgacgac gaggtcgggc
    18421 tggtgcgcga gcagttgccg cgcctgatgt gcggggtggc cgagctgcgc gtgccgctgg
    18481 tggcggaagt gggcgtcggc aagaactggg aacaggccca ctgaagcgcg tcagaacgta
    18541 aatttggtaa cattcaacct gggtttgtcg ctgaaagtac gatattcaaa aagttaagaa
    18601 ttaaaaagag aagcctgtag cagtgaaccg gtggcgggcc gacaggaggg ctcatgcgca
    18661 atctgcctac gttgatcgat tccagcagcg acacggccgt gatgattgcg gacgcgaggg
    18721 gtattgttac ctacgtcaac gtcggtttca cccgcctgtt cggctatcag cgcgacgagg
    18781 ccatggggca attgctgggc acgctgctgg tcggcgcgaa taccgacgtc aatacgctgt
    18841 accgcctgca gcacgccgac gacgtgggcc agaaagtggc cgacaacgtc atcctgctct
    18901 atcccaagac cggccggccc ctctgggcgg ccatgtcgat caaccatgac tacgatgcgc
    18961 agggcgaggt cgacaacacc ctcgtcatgc tggcggacat caccaccagc aagatgtacg
    19021 aagtcctgca gcacgccatc ctggacgccc tggcccacga gcagccgctg ggcgaggtca
    19081 tgaccctgct gtgcaaggag gtcgagaacc tggcccccga agtcgtgtcc accgtggtcc
    19141 gggtggacga gcggcaatgc ctgcgccccc tggcttcgcc cagcttgccc gagtcgtatg
    19201 ccgaccacct cgacggcgcg gccatcggcg tgggccgggc gtcctgcggc acggcggcct
    19261 ggctgggaga gtccgtcacc acctacgaca tcgccacgga tgagaattgg gacgggtttc
    19321 gcgacctggt gctgccgctc ggcctgcggg cctgctggtc caaccccatc aagtcgcacg
    19381 acggccgggt actgggcagt tttgcctttt actttcgcga gccccgggcg cccgacgagt
    19441 tccatcggcg cctgatcgat tcctgcctgc acttgtgcgc gctggccttc gagcgcgaag
    19501 aggcccggtc gaacatacgc cggctggcct tctacgacca gctcaccggc ctggccaatc
    19561 gcaatacgct cagggtcgag gccggcggca tgctcgagcg cgtccggcgc cagggcggcc
    19621 tgctggccct gctgttcatc gacctggacc ggttcaagac catcaacgac acccagggcc
    19681 acgccatggg caacctggtg ctcaacgagg tggccctgcg gctgggcagc aacgtgcgca
    19741 tctcggacct ggtggcgcgg gccggcggcg acgagttcgt ggcggtattg cccgattgct
    19801 cgatcgaaca ggtggcgcgc gtggccgaca agatgctgca ggcgttgaac cagccgatcg
    19861 atctgggcaa cggggtgagc gtgatcacgg gcgccagcat cggcgccgcg ctgtacccgg
    19921 acgatggcga cgacgtggac acgctcatgc tccacgccga catggccatg taccaggcca
    19981 agcacgacgg ccgggcctgc ctgcgcttct tccaccacga catgaaagac cgcacccgcg
    20041 agcgcgcggc gctcgagacg gcgctggggg agtcgctgcg cgcgggcagc ctgtgcctgc
    20101 actaccagcc caagatcaac cacctgggca tggtctacgg cgtcgaggcc ctggcgcgct
    20161 gggaccatcc gctgctgggc aagatcccgg cctcgcgctt cattcccctg gccgaggaaa
    20221 gccacctcat caacttcctg agcgaatggg ccatcggcga ggcctgccgg cagatggcgg
    20281 tctggcagag cgagggcatc gcggtgccgc acatggcggt gaacgtgtcg gccctggact
    20341 tcaagaaccg ggacctggcg ggcgtgctga gaagcgccct gacgcgcaac ggcctgcagc
    20401 cggcccagct ggtgctggaa atgaccgaag gcgtcatgtt cgacgatgcg ccggacacca
    20461 tggcgatgat ccagtccgtg cgggacctgg gcgtgcacct gtcgctcgat gatttcggca
    20521 caggctattc gtcgctgagc tacctgcacc gcttgtcggt caacgagatc aagatcgacc
    20581 gcagcttcgt gtgcgacctg gtgccgggca gcaagtcgca ggccctggtg cgcaccatgc
    20641 tcaacatcgg ccgcagcctg ggcttgaccg tggtggccga aggcgtggaa accgaggcgc
    20701 agcgcgattt cctgctgctc aacggctgcc tggtgatgca gggctatcta tacagccacc
    20761 ccatgtcgcc ccaggacttt acccggtgga tgcagggcag agtggtgccc gcgtaaagcc
    20821 gcaggcggct tgaaccgggg ttgaaatgct ataatgttgc acctcccggg cctgacggcc
    20881 acttcctgtt tttccccgtc ttcatgctgt tactctggat ttttctggcc accatcctgg
    20941 gcggcctgat cgccattggc attgcccaat ggctggccta ccgcgtcttt gcccactatc
    21001 tgcaccacat ggtcagcctg tcggtgggcg tgctgctgtc ggtggcgctg ctgcacctgc
    21061 tgcccgaggc cttcgagggc agcgatacca accctcgcac catgttcgcc gtgctgctgg
    21121 ccgcgctgct ggggtttttc gtgctggaaa aaatctcggt gctgcgccac agccaccatc
    21181 acgagggcga cggccaccat caccaccacg gccacgacag ccacgaggca ggcaagggcg
    21241 ggcgcctcat cctcgtcggc agctcgctgc acaatctgtg cgacggcgtg ctggtggcgg
    21301 cggctttcct gacggatccc ctgctgggcg tgttgaccgc ggcctccatc atcgtgcacg
    21361 aagtgccgca taagctgggc gatttcgtgg tgctgatcaa tgccggcctg gcgcggcgca
    21421 ccgccgtgct gctgattctc ttttccagcc tgtgcacggc cgtgggcggc gtcgcgggct
    21481 acttcgtgct gcatgaagtg caggcctaca cgccctacct gctggtggcg gcggccggca
    21541 gcttcctgta catctcggtc gccgacctca ttccgcagat gcacgagcgc gtccacctgt
    21601 cggacgcgat cccgcaagtg ctgctggtgg gggtcggcgt cgccatcatc tacggcgtga
    21661 ccaccttcat ggaaggcgtg gagcaccacg acgccgccga agcggctgtc gcgcacgctc
    21721 ataaataatt caggcaatcg tcgtatcatc ggcgggttca ccgttcagcc caggaggtct
    21781 tccatgagca ctgctcccgc cgccggcccc gtcgccgcga ccgtcatcca caccgatacc
    21841 cagggcctgg cccagggcga tttccgcctg gccgtccagg gcggcgccat cgacgcctac
    21901 tacgccgcgc ccgagggcaa gaaagaccta cccgtggtgc tcgtggtgca ggaaatcttc
    21961 ggcatccacg aacacatcaa ggacctctgc cgccgcctgg gcaaggccgg ctacctggcc
    22021 gtggccgcca atctgtacga gcgccagggc gacgcctcgg cctataccga cattcccaag
    22081 ctggtgtccg agctggtgtc caaggtcagc gacgagcagg tgctgtccga cctcgacgcc
    22141 agcctggcct gggccggcca gaacggcggc gacaccaagc gcgtgggcgt cacgggcttt
    22201 tgctggggcg gccgcctggc gtggatgtac gccgcgcaca acccggcggt gaaggccggc
    22261 gtggcctggt acggcaagct caccaccggc cacggcccgc tcatcaagac catggcggtg
    22321 gacatcgccg acaagatcca cggtccgatg ctgggcctgt acgccgagaa cgacgccagc
    22381 attccgctgg acagcgtcga ggaaatgaag aagcgcctgg ccgccgccgg caacgcggcc
    22441 gccaaggcct ccgagatcgt ggtctacccc ggcgcgcatc acggcttcct ggccgactac
    22501 cgccccatgt acgacaagca cgccgccgaa gacggctgga agcgcatgct gggctggttc
    22561 ggcaagtacc tgtaagcccg cgtccggagg gaggggctgt ggcggtttcg gtattcgacc
    22621 ttttcaagat cggcataggc ccctccagtt cccatacggt gggccccatg cgcgcggccc
    22681 tgctgttcgt ccaggggctg gcgcgcgacg gcctgctggc acgcacggcc caggtgcgcg
    22741 tcgagctgta cggctcattg ggcgcgacgg gcaagggcca cggcaccgac aagggcgtga
    22801 tgctgggcct catgggccag gcgcccgaca ccgtcgatcc tgccgccata cccggcctgc
    22861 tgcaggcggt gcgcgagacg cgcagcctgg tattgtcggg cgggcgcgcc gtgcccttcg
    22921 tcgaaaagga acacgtggtg ttctaccggc gcgaggccat ggccgagcat cccaacggca
    22981 tgaaattcca tgcctacgat ggcgacgggc aggccttgcg cgaatcccgc tacctgtcgg
    23041 tgggcggcgg tttcgtcgtc accgcgggga cgaccaacag ccaggtgatc gccgcgcatg
    23101 acagcctgcc ttttcccttc cgcacggccc gcgagctttt gcagatgtgc cgcgcaagcg
    23161 gcctgacggt ggccggcctg atgctggaga acgagctggc gtggcgttcg cgggaagacg
    23221 tcgaagcggg gctggaccgc atctgggcgg tcatgcagga ctgcgtgagc cgcggctgcg
    23281 gcatcggcca gcccgacatg gtgtgcgagc tgcccggccc gctgcgggtg cgccgccgcg
    23341 cgcccgagct gtaccggcgc ctgacgcgcc aggccgagcg cagcctgtcc gacccgctgg
    23401 ccgtgatgga ctgggtcaac ctctacgcca tggccgtcaa tgaagaaaac gccgcgggcg
    23461 gacgggtcgt gacggcgccg acgaacggcg cggccggcat catcccggcg gtgctgcact
    23521 actacgaccg tttcgtgccg ggcgccagcc aggctggcgt gcgcgagttc ctgctgacgg
    23581 caggcgccat cggcctgctg tacaagtaca acgcctcgat ctccggggcc gaggtggggt
    23641 gccagggcga ggtcggcgtg gcctgctcga tggcggccgg cgcgctggcc gccgtgctgg
    23701 gcgcggacgt cgagcaggtc gagaatgccg ccgagatcgg catggaacac aatctgggcc
    23761 tgacttgcga cccggtgggc gggctggtgc agatcccgtg catcgagcgc aacgccatgg
    23821 cctcggtcaa ggccgtcaac gcttcgcgca tggccctgcg cggcgacggg cagcactacg
    23881 tatcgctgga ttcggtcatc aagaccatgc gcgagaccgg cgccgacatg aagaccaagt
    23941 acaaggaaac ggcgcgcggc ggcctggcgg tgaatatcgt ggagtgctga ggccgcgccg
    24001 gcggcaagcg catgccggcg ttttactgcg ggtagcttcc gcccagcagc gcggtgagct
    24061 tgcgcgcatg ggccagcacg atgcggtcga agccgctttc caggcgctcg gccggcatgg
    24121 tgagggtgag cgcgcccacc agctccccgc cggcgccgaa gacgggcgag gccaccccgg
    24181 ccagctcggg caggcgatcc cccaccaggg tcacgacgaa ttcgcgccgg atgcgctgcg
    24241 agatcgggtc gttggcgtcc ggccaggcgc tgagcacgcg cccgcccgcg ccgcggtcca
    24301 tgggcagcag ggcgccgacg gccaggtggt cgcgcaccgg ccgcggcgag tcgacgcggt
    24361 gcaggcacag gcgctggtcg ccctggcgca cgtggaaggc cgcgctctcc tgcgtgcgcg
    24421 cgaccaggtc gcccagcacc ggcatcacca ccgaccccaa cgaaaaagac agcgcatgga
    24481 tgcggtgcag ccgctcgatg cccgcgccca gcgcgtagcg gccgtcctcg cgcttctgca
    24541 ccaggtgggc atgttccagc gaggccagca tgcgcagcac cgtgctcttg tgcaactggg
    24601 tgcgatgcgc gatctcggcc agcgtcagga ggggggcggt cagggaaaag gcatcgagca
    24661 gcgacagggc gcggtcgacg gcggcgacgc cgccgcttgc ggcgttctgg tcggccagcg
    24721 aggggatgga tgatttgcgg ggcatggaaa ccaggacgga tctcgggatg ttgcggggca
    24781 gcttgacatt ctaatgagga taaaacatca ttagatagaa cataattcta tctaatgaaa
    24841 ctgttcgtca ggagacaacc catgatccgt cgcgcctttc tggccgcgct ggcttgcgcc
    24901 gcgctgccgc tcgcttccgt ccccagcctc gcggcgccgg actatcccga tgcgcccgtg
    24961 cgcttcatcg tgccgttccc gcccggcggc ggcaccgaca acgtttcgcg cgtgctggcg
    25021 cacggcatcg ccgaagacac ggggtggaac ctggtgatcg agaaccacgc gggcgcgggc
    25081 ggcaacatcg gcatctcgct ggtggccaag ggcaagccca atggcctgat catcggcatg
    25141 ggccagacct ccaacctcgc catcaacccc acgctgtacg cgaccgtgcc ctacgacgcc
    25201 gcgcgcgatt tcacgccggt ggcgctggtg gccacgcagc cgacggtgct gatggtgcgc
    25261 gccgagtcgc cgttccatac gctgcaggac gtcatcgcgg cggccaaggc caagaccggc
    25321 tcgctgacca tcgccaattc cggcgtgggc acggtgagcc acctgtcggc ggtgctgctg
    25381 gccaaggtcg ccggcgtcaa gctgctggac gtgccctatg cgggcgcctc gccggcgatc
    25441 accagcctgg ccggcggcca ggtcgacctg agcatgagcg cgccgtccag cgtggcgccg
    25501 ctggtcaagg cgggcaagct gcgcgcgctg gcggtcacct ccgcccagcg cctgcccatc
    25561 ctgcccgagg tgcccacggt ggccgagtcg ggctaccccg gcttcgaggc gctggactgg
    25621 aaggccgtgg tggcccctgc cggcacgccg gccgatgtcg tcgccgcgct caatgccgcg
    25681 gtgaacaagg cgctgacgcg gcaggacgtc atcaacgcgc tgctggccga cggcagcacc
    25741 gtggcgggcg gcacgcccga gcgcttcggc cagttcctgg cgtcggaaca gaagcgctgg
    25801 ggcgacaccg tgcgcgaatc cggcgcccgg gtcgattgag ccggcagatc gaatcatcca
    25861 cggaagtccc catgaccaaa cccctgcggc acgtgcgcgt gctcgatctc accaatgtcc
    25921 tcgccggtcc gttctgctgc caccagctgg cgcacatggg cgcggaagtc atcaaggtcg
    25981 agacgcccgg caccggcgac ctggcgcgcc agctgggggc cgacgccgag ctcaatcgcg
    26041 ccctgatggg cgtgtccttc ctggcgcaga acgccggcaa gcgctcgatc acggtcaacc
    26101 tcaagcatcc gcgcggcaag gaagtgctgc tgcgcctggt cgagagcgcc gacgtgctgg
    26161 tcgagaactt ccggcccggc gtgatgcagc gcctgggcct gggcttcgag gtgctcaggc
    26221 agcgccagcc caggctggtg tattgcgcga tctccggctt cggccaggac gggccgctgc
    26281 acgacctgcc cgcctacgac cagatcatcc agggcatgtc gggcgcgatg agcgtgacgg
    26341 gcgacgccgg gagcgcgccc ttgcgcgtgg gctatcccgt ggccgacacc gcgggcgggc
    26401 tgaccgccgc attcgccgtg gcggccgcgc tggccgagcc caaccgcgag acggcgcgct
    26461 acatcgatgt gtccatgctg gagtcggtca tgaccaccat gggctgggcg atctccaatt
    26521 acctggtggc gggccgcgag cccaggccca tgggcaatga gaacgtcacc gccagcccgt
    26581 cgggcacctt ccgcacgggc gacgggctgc tcaacatcgc ggccaacaag caggagcagt
    26641 tcgaggcgct gtgccgcgtg gtcggccgcc ccgggctgat cgacgatccg cgctaccgcg
    26701 agcgccaggc tcgcctgcag caccgctacg agctgaaggc cgagatcgag gcggcgctgg
    26761 ccgcggacag cgcggagcac tggtggccgc ggctcaccga ggccggcgtg ccgaccgggc
    26821 cggtgtactc ggtgccgcag gcgctggcgc atccgcaggt gcgcgaccgc ggcctgatcg
    26881 ggcagttcga cgacgcgccc ggcgtggggc gcgccgtgcg cgtggtgcgg gcgggctaca
    26941 aggtcgacgg ccgcacgcca tcggtcgaca cgccgccgcc cacgttggga caacacaccg
    27001 atgccctgct gcgcgagctg ggctacggcg aagacgagat acgcgcgttg cgcgaggagc
    27061 aggccttatg agcgaaaccg catccccatc ccccggcgcc gccgaggtcg agaacgccag
    27121 cagcgcctgg tggcgcaccg gcgtcatcga catgcagccc ggcgtcatcc gctaccgcgg
    27181 ctatgccatc gaagacctga tcggcaacgt cggcttcgcc cagatgatct ggctgatgct
    27241 gcgcggcgac ttgcccacgc cggggcaggg cgcgctgctg gaggccgcgc tgatgtcggc
    27301 ggtggaccac ggtccgcagg cgcccagcat cgccatcgcc cgcatggccg cgacctgcgg
    27361 cgtgggcctg aacaacgcca tggcctccgc cgtgaacgtg ctgggcgacg tgcacggcgg
    27421 cgcgggcgaa caggccgtgg cgctttaccg cgacgttgcc gcgcgcctgg acgcgggcct
    27481 gccgcttgcc gaggcggcgc gcgccgcctt gcgcgactac gtcgaactgc acggcaagtt
    27541 cgtctcgggt ttcggccatc gcttccaccc gctcgacccg cgctcgccgc gcctcttgca
    27601 actggtggac gacgcggccg ccaggggcga ggtgtcgggc cgctacgccg ccattgcgag
    27661 ggccatcgag gccgagctgg ccgcgcagaa gagccggcct atccccatga acatcgacgg
    27721 cgcgacggcc gtcatctatg ccgagctgga ttttccggcg ccgctggcgc gcgggctgtt
    27781 ctgcctgtcg cgctcggtgg gcatcctggc ccatgcctgg gagcagacgc agcagggcgg
    27841 gcgcaacaag gggccgattc cgcggcagta cctgccgcag tacgagggcg tgccgccgcg
    27901 cgagctgccc gattgggcga agcagtgacg gctcaatgaa aaatgcccgc tttagtcgaa
    27961 gcgggcattt tggtctttac aggctttcct cgttgacgag gccttccttg ccatagaact
    28021 tcacgccgat ctcgatgcgt tcgcgccctt gcgcgcggcg atggcggttg ctgtcgcgca
    28081 ggctgtacac gcagccgcag tattcctgct ggtagaagtt ctcgcgcttg ctgatctcga
    28141 tcatgcgctg cgagccgccg cccttgcgcc agttgtaggt ccagtacacc aggtcgccgt
    28201 agcgggccgc ggcgcgcgcg cctgagccgt tgatctggtt catgtccttc cagcgcgaga
    28261 tgcccagcga gctggtgatg gtgtcgaagc cgtgctcgtg cgcgtacagg gcggtgcgct
    28321 cgaagcgcat gtcgaagcac ttggtgcagc gctcgccgcg ctcgggcgaa tcttcaagcc
    28381 ccttgacgcg ctcgaaccag ttgtccacgt cgtagtccgc gtcgatgaac gggatatcgt
    28441 gctgctcggc gaagcggatg ttctcgttct tgcggatctc gtactcgcgc acgggatgga
    28501 tgttggggtt gtagaagaag atggcgtagt cgatgccgga ggccgtcatg gcctccatga
    28561 cttcacccga gcagggcgcg cagcaggagt gcagcagcac tttctggcgg ccttcgggaa
    28621 gtgcgagctg ggggcggacg acgtcggtca tggctggggc agggcgtaaa acggcaggga
    28681 tatcaacatt ttacgcgatc tgcttggaga tggccgaggg cgcgcggggc acgccctgtc
    28741 cgtcatttac tggcggctgg tgacttcgac caggtggtag ccgaactggg tcttgaccgg
    28801 gccttgcacc tcgttgaccg gggcgctgaa cacgaccttg tcgaactcgg gcaccatctc
    28861 gcccgggccg aagctgccca ggtcgccgcc ggcctggctg gacgggcagc tggaatgctg
    28921 tttcgccaga tcggcgaagc tggcgccgtt ttgaatggcg gttttcagtt cattggcctg
    28981 ggcttcggtc gaaaccagga tgtggcgggc ggtggcttgt gccatggcgg ggactcctga
    29041 aatgggggtt ttgggatcgt actgcatagc gcagggggtg ggccgctttc ggccataaag
    29101 cgccgtgttc acggcgattt catatcggtg ccgctttgct tcttcttcat cgagtttgca
    29161 agacgctgca ccagatcggg aaatatccgc ctttcctcgg ctgaccagtc cttaatcagc
    29221 tcgcctagca gtttgcgtcg cgcgtcggtg atgcgcgcaa gcgtgcgtac gccgacgccg
    29281 gtcaccgagg cggcccgtac gcgcttgtcg ttctgcccgg ccgggcggca tacaaacccg
    29341 agctgttcga gcttcgcgag ctggcggctg actgtggaag ggtcacgacc cacctgctcg
    29401 gccagttgag cgacgccggt cgtgcccgcc gcgtgtaacc gaaccagcaa tggaaacaag
    29461 gcccgatcca gcgagacacc ggccacgctc aacagtacct catcctggcg tgggctgttc
    29521 aggcagccgg ttagctcgat caatgccgac gcgatggcct ctacattccg tgcactacgc
    29581 atgctttaca tcgtcctttc gctcgcccat aattcgtgca tcatacatgc aaatggatag
    29641 gtgattcgat ggaccgagcg aaaaccgtgc tgctgatcat gggaagtacg cgcgcggcca
    29701 gaatttgccc aaagatcacg gcgtggatcg cggaaatcgg gcaaacctgt acaggcttag
    29761 cctacgaaac cgttgacctg gccgactggc tgctgccgat ggacgacgag gcggcgctcc
    29821 ccgcgctggg accgtatgcg cagccgcaca cgcgcgcctg gagcgacaaa atcaagagcg
    29881 cggatgcggt ggttttcgtt gcgccgcaat acaactgggg ttatccggcg gtgctcaaga
    29941 atgccatcga tcatctctac catgagtggc gagacaagcc ggccgcgatc gtgacctatg
    30001 gcggccacgg cggcacccga tgcgctaagc agcttcgaca ggtcgcgact gcagtgaaga
    30061 tgcgcatggt catgaaagcg ccagccatca ggctatcgga ggcagtgatt cggcagggcg
    30121 cctcgtttat cccggaacgg gatttcctgc catatcgacc gcaaatccag catgcgctcg
    30181 tggaactggc tgcccaggtc gccggacgtg aacgcttcct gacgcgggta ggccggaagt
    30241 ggaaggcatt tctctttgcc atgacctgat acgccggata gggacacggg cgtatggccg
    30301 tttacctttg gaaagtgctc atgaggttac gctggggcgg atagccatga ctcaagcggc
    30361 ctccggcttt gcagtgcgct ggcggcgcag ttcgaccccc tgcgccgcgc cgatggcgcg
    30421 ggcgcgcagc tggccgcagc cgccgtccac gtcctgcccg gcgctgttcc tcaccttggt
    30481 cagcacgccg cggctgtgca gggcgtggac gatctcgcgg atacgctcgg gatcggggcg
    30541 ctggtagtcg tccccttcga gactgttgaa ggggatgagg ttgagcacgc cgtacttgcc
    30601 cttgagcagc cgcaggattg catcgagttc gtcgtcgccg tcgttgacgc ccttgagcag
    30661 cgtccactgg tactggatcg ggtagtcggc gtcgcgggcg tatttttcgc cgagctcgat
    30721 cagttcttcc ggcgcgattt tcggcgcgcg cggcagcagg tgttcgcgca gttcggcctt
    30781 ggtcgtgtgc agcgacagcg ccagcgcggg cttgacgcgc tgtcgcggca gggcttcgaa
    30841 cacgcgccgg tcgcccacgg tggagagcac gaggttcttg tggccgatat tgccttcggt
    30901 gccgagcagg tcgatggctt cgagcacgtt gtcgaggttg tgcgccggtt cgcccatgcc
    30961 catgaagacg accttcttca ccgcgcgcag acgccgcgcc agcacgacct gggcgaggat
    31021 ttccatgctc gagacctggc ggatcaggcc gctcttgccg gtcatgcaga agcggcagcc
    31081 gaccgcgcag ccgacctggg tggagacaca gagtccgtcg cgcggcagca gcacgctttc
    31141 caccatctgt ccgtcggcga gttcgacgag caggcgcgag ccgtcgttgc cggcatgttc
    31201 cgagcggatg cgggcaaggc cgtccagttc cgccgtcaag gcgggcacgg catcgcgcag
    31261 cgcccgcggc aggaagtgct cggagcgccg ccgccgcgtg ccgctgtcga gcgcttgtcc
    31321 gttcagccag acgcgcacga cccggccgcg atggaccggc tgggcgccga gggcggcgag
    31381 gcgttggtcg aaatcggaga agcgcatggg gagcggattc tactctgcgc ggctcgcacc
    31441 cgccgggccg tcagttcggc attttttcct cggggcgcaa cgtcaagact cgcacgccgt
    31501 ttcgggtcac ggctacggtg tgctcgaatt gcgcggacag ttgcccgtcg cgggtgacga
    31561 ctgtccagcc gtcttcttcg gtccgaacgg catcccgccc ttggttgatc atcggttcga
    31621 tggtgaacac cattccttcc cgcagcacca gacccgtttg cggcttgccc caatgcagca
    31681 cttggggcgg ctcgtgcatg tcgcgcccaa tgccgtgccc gcagtattcc ctgacgaccg
    31741 aatagccgtt tctccgtgcg tgccgctcga tggcgtgccc cacatcgcca agcctggcgc
    31801 ctggacgaac ggccttgatt ccttgccaca tcgcttcgta ggccacctgc accagccgtt
    31861 tggccggcgg tggcacctct ccgacaagat aggttttgct ggaatcagcg atatagccgt
    31921 ttttctccaa ggtgatatcg aaattaacga tatccccgct ttgcaataga tcggtcgtgg
    31981 acggaacccc gtggcaaacc acgttgttgc gcgaggcgtt cagtgcatag gcatacccgt
    32041 actgcccttt gcttgccgga cgcgcgttga gttcatgcac gatgaggcta tcgaccagat
    32101 tgttgacctg catggttgtc atgccgatca agcccagccg gtccacgtag ccaaacacat
    32161 ccgccagcaa cctgcctgat tccgcgagca atgcgatttc ttccggtcgc ttggtcatgt
    32221 cgccgccacg tcgacggccc gcgccacaac gcccgcggct cgcagttccc gggccatgat
    32281 ctcattgaaa ctctgcgtcg gattcatttc gcacaacctg cctatcctga tccagaaagc
    32341 cgcttgcgcg ttgatcgacc ggcacgacac ggcgctcgct ttgcgtagtt gatcgtgcag
    32401 atcatcgtcg atattcacaa tgcccatgcc gccctcaata tatgaaacgt atatgaatca
    32461 tattgtcgaa cccgatggca ttgcaagcga tccttgccgc ttcgtcgacc cggcgaatgc
    32521 ctgacatgaa tgggttccag actcgcacgt caccggccaa gacgaccggt cacgccgtcc
    32581 ccaaggcgga aagtcctaca attccgcttt gcaacaatcg ggcgcgggta ccgaaccggc
    32641 acgctacccc ggcgcaagtc atgactactc aagctttctc gattgcctgc ggcatcgact
    32701 tcggcacgtc gaattccgcc gtgggatggc accgcccggg gcttccctcc ctgctggccc
    32761 ttgaagaggg caagccgacc atcccgtcgg cgatcttctt tcacgacgaa gacgccgccg
    32821 tcagctacgg ccgggccgcg ctggcggact acctggcggg ctacgacggc cgcctgatgc
    32881 ggtccatgaa gaacctgctg ggcagctcgc tgatcgacgg ccatacggaa atccgcgggc
    32941 gcggcgtgcc gtttcgcgta ttgctgaccc atttcatcca ggaattgagg acgcgggcgc
    33001 agcaggccgc cggccgggaa ttcacgtcgg tggtcatggg ccggccgacg tttttcgtcg
    33061 acggcgacga ggtcgccgac cgcgccgcgc aggataccct gggtgaaatc gccgccgccg
    33121 tggggttcac ccatatcgaa ttccagtacg agcccctggc ggcggcgttc gactacgagt
    33181 cgcagatcag ccgcgaggaa ctggtgctgg tcatcgacat cggcggcggc acgtccgact
    33241 tctcgctgat acgcctgggc ccggagcgcg cggccatggc cgaccggcgg gcggacatcc
    33301 tggcgcacgg cggcgtgcac atcggcggcg gcgacttcga ccaatacctc agcctggact
    33361 cgttcatgcc cttgctgggc ctgggcagcc aattgcgcag cggcaaggac gtgccgtcca
    33421 cccagtacgt caacctggcc tcctggcaca cgatcaactt cgcgtatacg aacaaggcgt
    33481 tggaacacct gtcgtacatc cgcgccaatg cggccgagcg cgacaagatc gacctgctgc
    33541 tgaggctggt gaaggaacgc gccgggcatt ggctggcgct gcaggtggaa aaaaccaaga
    33601 tcgacctgtc cggcgcgccg tccacgcaaa tcggcctgga gcggatcgac cgcgaccggg
    33661 tcctggccgt ttcccgggac gacctcgacc gcgccgtcgc gccgctgatc gaccggatcg
    33721 aaaccaccgt cgccacgctg ctgcgcgacg cgggcgtgcc gttggacacg atcgacaccc
    33781 tgttcttcac gggcggatcg agccgcgtcc cgctgctgcg ccaacgcatt tccgcgctgc
    33841 ttcccggcgc ccgcagcgtc gaaggcgacc ggttcggcag cataggcacg ggcctggcgc
    33901 tggatgcggc gcggaagttc ggataagggc gggacgccgc cgtcccgcct gcgcaagccg
    33961 gaaatcccgg tgagcgcgta aggttgtcac gcgccgggcg cgcgccggca gttactatcg
    34021 cgccctctcg cttccccctc agcagctccc ccatgcatgc acactctgcc gcgcaccgcg
    34081 gcgcttctcc tttgcgcgcc tggctggccg tcggcgccgt cacgctggct tcgttcgcct
    34141 tcgtcaccac cgaattcctg ccggtcggcc tgctgcccgc cattgcccag gaggtcggcg
    34201 tccagcccgg caccgccggg ctgatggtga ccatccccgg cctgctggcc gctttcgccg
    34261 cgcccggcat gatgctgggc gccggccgca tcgaccggcg ccgcatcctg ctggtgctgt
    34321 cggtgctgct ggtggcgtcc aacatgatct cggcttttgc cggcagcttt cccgtcatgc
    34381 tgctgggccg cgcgctgctg ggcgccagcc tgggcggctt ctggacgctg gccctggccg
    34441 cggccggccg cctggtgcgt ccgcaagaca gggcccgcgc caccgccgcc atcatggccg
    34501 gcgtgacctg cgccacggtg atcggcgtgc cgctgggcac cttcatcgcc ggcctggctt
    34561 catggcgcgt ctcgttcatg gccaccggcg cgctggccgc cgtctccctg gtcgcgcagt
    34621 gggtgctggt cccctcgctg ccgtcgagcg cggccctgcg cgtggccgat ctgctcgaac
    34681 tgtggcggcg cccgcactcg cgcaagagcc tattgatggt cgtgctggtc ttcgccgcgc
    34741 atttctcgtc gtacacctat atcgcgccgt tcctgctgac gcgcgccggc ttcagcctgc
    34801 agagcattac ctgggtattg ctgggcttcg gcctgatcgg ctttgtctcc aatgccgtcg
    34861 cctcggccac ggccagccgc cacctcaagg ccacgctgct gaccatcgtg accctgctga
    34921 tgctggcctt gctggcgctg ccctggctgc aaggcgccgc gcccgcggtg acggcggccg
    34981 tgctcgcctg gggcatcgcc ttcggcgcga ttccgctgtg catgagcctg tggatgcatg
    35041 ccgcggcgcc cgacctgccc gaagcggggt cggccacctt catcgccacc atccaggtgg
    35101 cgatcgccgt cggttcgtcg gtcggcggcg ccatcgtcga ccgggtcggc attcccgtgg
    35161 acttccggtt cggctgtgtg ctggccgtgc tcggcctggc catggtggcc agtttcggcg
    35221 cgcggcccaa ggccgcggcc tgagccgctt agtcgaaatc gggcgcgaat agccaactgc
    35281 ctcaacccaa gacaacccgc cacaattcct tcaccgtttc ccgctcaacc gccacgctct
    35341 gcgcgggcac ccgcgccctc tccacccctt gcagccgcaa ctcgtgctgc acccgccgca
    35401 gggtgcggta ggcatttccc gcgtgctgcg ccaacgcggc gggaatcaac ccggcttccc
    35461 ccgcgatctg cagcagcgcg atgtttccca ggttttccat cagctcggga tgcatcgccg
    35521 actggctcag caccagatac tgcgtcacga attccacatc caccatcccg ccgcggtcgt
    35581 gcttcaggtc gaacagtcct gatgggttgg ggtgtcccgc gctgatcttg ccgcgcatgg
    35641 ccaggacttc cctgcgcagt ttgtccatgt cgcgcggcat ggcgaggatg tcgcggcgga
    35701 tgtgttcgaa gcgttctccg atcgcaacgt cgcccgccgc gtagcgcgcc cgcgtcaggg
    35761 cctggtgttc ccaggtccag gcgtgctggc gctggtattg ctcgaaggcc gccacggaca
    35821 cggccagcag gccggcctcg ccgtcggggc gcaggcgcag gtcgacgtcg tacagccggc
    35881 ccgacgaggt cagggtcgac agccaggagc tcatgcgccg gccgagcttg gcgtagacct
    35941 cggccgcgtc gtcgcgcggg tcgtcgaaga ggaagaccag gtccaggtcg gagatgtagc
    36001 ccagctcctt gccgcccagc ttgccgtagg cgatgacggt aaagcggggc acggcatcgg
    36061 gttcgcggtt gaccaggagc cagacgcgcc gcaaggtttc ggccaatagc aggtcggcca
    36121 gggccgagag ctggtcgccc aactgctcca cggtcagcgc gccttccagg tcctgcgcca
    36181 gcagctggaa gctcgcctgg cgctgcatat cgcgcatcag gttcatctgc cgttcgacat
    36241 cgggcgtgcc gtcgggcagc aggctggcgt cgaggtcggc ggccagctgg cggccgatct
    36301 gcggccagtc agggggggcg agcagggtgt gccagtcgat caggctgtcg agcagcaggg
    36361 ggtgctggcg caggtattgc gcggcccagg ggctggccgc cgtgatgcgg gccacgcgcg
    36421 ccagggtgtc gggatattcg gccagcagcg ccaggtaggc gctgcgttgg gcgatggcct
    36481 cgatcaggtc gagcaggccg atggcgctca ccgaaggcgc gcgggtccgc gacgcggcgc
    36541 gcagggccgc cggcagcagg gcgtcgacgc ggcgccggct ggtctcgggc aggctctgca
    36601 tgcggtagct ggccagcagc gcttcggtgc ggcgcaggag ttcatcggcc tggtcgccga
    36661 actgcgcgcg gatctggccg gccaggttct ggtcgctggt gctggcgtcc tcgaccggcc
    36721 ggcccgtgtt ggcccgcacc tggtcgtcca ggtcgcccat gccggcggcg tggaaggcat
    36781 tgcgaaaggc ctcggagacc acggcgcggt gcgcggccag cgtgtgttcg aaagcctcgg
    36841 gcgcgaaccc cagcgcggcg gccagctcgg cgcgccgctc gggctcgccg ggcagcaggt
    36901 gggtctgttc gtcctcgcgg tactgcagcg cgtgctcggt gcggcgcagg aatcgatagg
    36961 ccgcctccag cttgtcggcg tcgtcggcgg cgatcaggcc ggcatcgcgc tcggcgtgca
    37021 gggcggcgat caggccgcgg cattggaggg cgggcatgcg tccgccgcgg atcatctgcg
    37081 agagctgcac cacgaactcg atctcgcgga tgccgccgtc gcccagcttg atgttgttgg
    37141 cggtgtccag gccgttgcgc gccagggcgc ggcggttcca gtcctggcgg atgcgttcgc
    37201 gcagggcgcg cagcgccgcc agcgcatcga agtcgaaata cttgcggtag acgaagggcc
    37261 ggcgcaggtt ctcgaaatgc tgcgcctgcg cttcgggctg gctgtcgggc caggcccgcg
    37321 cctcgatcag gcgggccttg agccaggcat agcgctccca ctcgcggccc tggccgatca
    37381 ggtagtgctc gaaggcttcc aggctccagg ccagcgggcc ggcgtcgccg tcggggcgca
    37441 ggcgcaggtc ggtgcgaaac acctggccgt cggcgtcgag ctccgacagc atgggcatca
    37501 tgcggcgcgt cagccggccg tagaactcgt ggtggctgat ggggcgcggt ccgtcggtct
    37561 cgccttcctc gccgtagagc atcaccaggt cgatgtcgga ggacacgttc aactcgcccc
    37621 cgcccagctt gcccatgccg acgatcagca tctcctgggg cgcgccggtg gccgggtcgc
    37681 gcgggatgcc gtgcgtctcg gccagctcgc gggcggcgct gcggtaggcg gtcgcgacgg
    37741 ccaggtcggc cagggccgtc atggcgccca ccacttcttc cagatcggcc aggccggcca
    37801 ggtcgcgcac cgtcagcagc gtgaagacgc gttcgcgcag ccgccgcaag gcctgccggc
    37861 acaggtcgcc gggcagggcg gccgcggttg cccggccggc ggcgagttcg gccagccagg
    37921 ccgacaggac ggcgggcgtg acgggctgct gcgcctgctc ggccagccag gcgggcagat
    37981 cggggcgcgc catgatgcgg cggcgcagat agccggacca ggccagggcg ggggcgagca
    38041 aatcggagga cggcatggtc gtcaatgtgg ggggggggac gtcaagacgg aatcgttcgt
    38101 ttcctgtata agagggacga tactgtaaat tcgtttcttt ctgtctaccc gtgcatgtga
    38161 ccaccaaagt cctgcgttgc ctgttctggg gcgtcctggc ggtcttcgcg gtggcgttgg
    38221 cggcctgcct gggcctgcgt tatctggtgc tgcccaagat agaccagtgg cgtccccgga
    38281 tcgagcagta cgcatcgcag tccctgggct cgcccgtccg catcggttcc atcgcggccg
    38341 actggagcgg cctcaatccg aggctgcgcc tgtcctccgt gcagttctac gacggggccg
    38401 accaaccgga gcccacgatc accctgcctt cggtcgctgc cgtggtggcc tggcgcagcg
    38461 tgctgcgcat gacgccgcgc ctgctgtcgt tggacatcga aggcgccgac ctcaccgtgc
    38521 ggcgcgacgc cgcgggcctc ctgtgggtgg ccggcatgtc gttcgacccc gccgcgcccg
    38581 cggccaaggg cgactcgccg gtgctggtct ggctgtcgca gcagcgccag ctgtccttgt
    38641 ccggcaccac cttgcattgg ctcgacgagc agcgccaggc gccggaactg gtgctcagcc
    38701 acgtggatgc cgccgtgcgc aatggcgcgc tgtcgcatcg ctttgccctg catgcgacgc
    38761 cgccggccgc gctgtcggcc ggcgtgaccc tgcgcggcga aatcgagcgg cgcatcttca
    38821 cgctcaacag ctccgacccg gcgagctgga acggcaattt ctacatgcag ctcgacgacg
    38881 ccgagcccta tgcctggtcg ccctgggtga cgatgcccgc cgtgcagggc cggctggcgg
    38941 cgcgcgcctg ggtgcagctg acgcatggcc agatcggcaa cgtgacgctg gacgcggtgc
    39001 tgcgcgggct ggacacggcc ttgccgcacc aggagggccg cctggcggcg aagcaggcgc
    39061 acttgcgcct ggacggcatg ccaggcgacc tggatgcctt gcccgccgac gccttgccgc
    39121 tgcccctggc gcgcagcgct tccgggcagg gcatgtcgct gcaggtccag gcgcaggggc
    39181 tggcggccac cttgccgacg gtgttcgata cgcccgatct gcgggtcgac gatctgcaag
    39241 tcgatgccag cctgctgcat ccgcccaggc agcctttgtc gctggacctg cggcaactgc
    39301 aactgaagaa cggcgaggcc gaactccagt tgcaggggcg ctggcgcgac gaaggcgcgg
    39361 gcggcgaggg cacggccgac ctgcaaggca agctcgtgcg gatggccatg cccgccatcc
    39421 atcgctactt gcccctgacc gtctcgcagt cgacccgcca ctggatggcc cagggcctgc
    39481 aggcgggcga ggtgcgcgat gcctcggtgg tgctcaaggg gcccttggcc gagtttcctt
    39541 tcacgcagcc gggagagacc ggccagttcc gcgtggcggg ccgctaccag gaggcgcgcg
    39601 tcgattacgc gccggcgcgt cccgaccgca agggctggcc cgtcctcagc gccatgtcgg
    39661 gccccttcgt ggtggaccgg gccagcctga cgctggacag cgccggcggc gtggtggaga
    39721 ccggcccgtc gcagcaggtg gtcctgcacg ccgtgcatgc cgccatcccg aacatggagc
    39781 acgatgcgca actggacgtc acgggcgaga gttcaggcgc ggtgccggcc tatctggcga
    39841 tggcgaccaa ctcgccgctg ggcggcctgc tcgacggcgc gctggaccag tcccaggggt
    39901 cgggcgactg gcaggtggcc atgaagctgc acgtgccgct cctgcacccc gacgacgcgc
    39961 agatcgacgg ccacattgtc ttccagggca ataccttcca gctgctgccc caattgcccg
    40021 ccatgagcgc cctgcagggc gacctggcgt tttccgatct cggcctgcag gccaacgata
    40081 ttcgcggcac tttcctgggc gggcccgcgc gcgtgtcggg caagctgggc catggcgaat
    40141 ccctggcctt ctccggcacg ctggccgcga cggccctgga cgaactgggc aagctgcccg
    40201 cctggcagcg cttttccggc aagacggcct accagggcaa gctgtcctat gccaagggcg
    40261 gcgatgtgga cgtgtcggtg cagagcgacc tgtcgggcct ggccatcgac ctgcccgcgc
    40321 cgctgggcaa gacggcgtcc gccaccttgc ccctggccct gcagtggagc cccgccacca
    40381 accgcggcca tagcggccgg cgctggctgt cgggcggcat cggcgagaac atcaacctgc
    40441 tgttcgagcg cgacccctcg gaccgcggcg cgtatttcgc gcgcggcgcg atcggcgtgg
    40501 ggcggccggc caccctgccg acccgcggcc tgaacctggc gggcacgctg gacacgctcg
    40561 ataccgatgc ctggaacgat gccttgaaaa gcttttcgcc cgcgccggtg tccgcgccgg
    40621 tgcccgcgcc ggccaagggc aaggccgcgc ccaagccggc gccgccggca ttgccggggc
    40681 tggaactggt caacctgtcg gtcaagcgcc tggtcgccgc gggccaggat ttcgacgaca
    40741 tcaccctcta tgcccagcgg cccgcgcccc agcaatggcg cgtgtcgatc gacgccaagc
    40801 aggccgccgg cacgctggaa tgggccgagg cttcggatgc ggcctcgggc aagatcacgg
    40861 cccgcttcga tcgcctggcg ctgggcaagg cgggcgaggc cggcaaggac gtcaagcccg
    40921 atggcgacaa ggaaagcgag ctgtccgaca tccccggcat agacttgcag gccaagcagt
    40981 tcgtgctgta tgggcatgac gtgggcagcc tggacctggt cggcaccaac ctcgagcgcg
    41041 gcaatctatg gcggctggac aagctgcacg tcgccaatga gtcggccacg ctggacgcca
    41101 ccgggtcgtg gcgcctgagc ggcaccgagc gcggcctgag cgcggatgcc agcatcgact
    41161 tcaaggacct gggcgccatg ctcgagcggc tgggcctgca gtcggtcagc agcggccacg
    41221 gcacgatcaa gggcaagctc ttctggcgca acctgccgtg gcgccacgac aaagccgaca
    41281 tcgaaggcac catgcacgtc aacctcgaga aaggccgctt cgtgcacctg agttcgcgca
    41341 catcgcgcgt gctggagctt ttgtcgctgc agtccctgca gcgcctggcc accctgagcg
    41401 ccaatcccgc cggcctgctg cgcgagggct ttcccttcga cagcgtcacg ggcgacatgc
    41461 gcttgaccgg cggcgtgatg agcatcgacg gctacaaggt caacggcccg gtggcggcca
    41521 tcggcctgga aggcaggacc gacatcatca aggagctttg ggacctgcac gccgtggtgg
    41581 tgcccaacct ggacgccagc ggcgcggcca ttgccgcggc gctggtcaat ccggtgatcg
    41641 ggctgggcgc cttcattacc cagtggctgc tcaagcagcc gctggcccgt gccatgacct
    41701 cggaatacca tgtcaccggc agctgggacg accccaaggt cgagacggtg gaaacgaaag
    41761 cgccggccgt ggaaagcgcg ccggcgacgc actgattttt tgccttgggg cgcggcgcgg
    41821 gggcttttac cgccgcgccg ggcaggggct tacttcttca tcatgtcgaa gaattcggag
    41881 ttggtcttgg tggccttcat cttgtcgagg atgaactcca tcgattggat ttcgtccatg
    41941 tcgtggatga acttgcgcag cacccatacc ttctgcagca gttcgggctt gatgagcagc
    42001 tcttcgcggc gcgtgcccga cttgttcagg ttgatggccg ggtagacgcg cttttcggcc
    42061 aggcggcgct ccaggtgcac ttcggagttg ccggtgccct tgaattcttc gtagatcact
    42121 tcgtccatgc ggctgccggt ctcgaccagc gccgtgccca ggatggtgag cgagccgcct
    42181 tcctccaggt tgcgcgccgc gccgaagaag cgcttggggc gctgcagcgc gttggcgtcg
    42241 acgccgccgg tcagcacctt gcccgaggcc ggcaccacgg tgttgtaggc gcgcgccagg
    42301 cgggtgatgg agtcgagcag gatgatcacg tccttcttca tctcgaccag gcgcttggcc
    42361 ttctcgatga ccatttcggc cacctgcacg tggcgcgtgg cgggctcgtc gaaggtggag
    42421 gccaccactt cgccgcgcac cgtgcgctgc atttcggtca cttcttccgg gcgctcatcg
    42481 accagcagca cgatcatgac ggcatcgggg aagttggtgg tgatggcatg cgcgatgtgc
    42541 tgcataatca ccgtcttgcc cgacttgggg ctggc
//
LOCUS       DASWPO010000028         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2505128, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000028 DASWPO010000000
VERSION     DASWPO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2505128"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1324
                     /locus_tag="VGC69_01390"
     CDS             2..1324
                     /locus_tag="VGC69_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY0293967.1"
                     /translation="MRHTGRLSRKSLRLILLLIGAALVALVSLGFARMADAALEWNRR
                     WTSAHGWLAFIVLPLGLLALRWLTLRFAPYAGGSGIPQVIGALSLPPGRPQWRLVSMQ
                     TAWKVPLTFLGMLMGASVGREGPSVQVGAAVMLAWSRLCVRLGVPLRGLDLRELIAAG
                     AAGGLAAAFNAPLAGVVFAIEELGRGIALRWQRRVLIAVLTAGFLVVAVAGNNPYFGH
                     FGGAAFAHGMLAWTLLCCLVNGVLGGVFARLLEKGLAGSLPEALGAAVRRHPYWLAAG
                     MGLLMACLGMLTAGAVYGTGYAAAAGMLAGEPAPVAGFGLAKLIATVASYWAGIPGGI
                     FTPALTTGAGIGHQIWHWAGAGVDQRVLVLLSMAAFLAAATQAPLTASVVVMEMTGSQ
                     PMLFWLLAASLGASVVSRQFSPQPFYHLMAGRFRRQTLAAEAFASKPA"
     gene            1418..2623
                     /gene="pcaF"
                     /locus_tag="VGC69_01395"
     CDS             1418..2623
                     /gene="pcaF"
                     /locus_tag="VGC69_01395"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY0293968.1"
                     /translation="MTTHAFICDAIRTPFGRYGGALSSVRADDLAAIPLKALMARNPG
                     VKWEELDDVLFGCANQAGEDNRNVARMALLLAGLPIEVPGATINRLCGSGLDAMGTAA
                     RAIAAGQASLMLAGGVESMSRAPFVMGKADTPFSRNAAIYDTTIGWRFVNKLMKAQYG
                     VDSMPETAENVATDYGVSREDQDKFALASQEKTVRAQQSGFFDAEITPVSIAQKKGDP
                     IQVVKDEHPRQTSLEALGKLKPVVREGGTVTAGNASGVNDGSAALLLADEKGAARHGL
                     KPRARVVGMATAGVAPRIMGMGPVPATRKVLAQTGLTIDQLDVIELNEAFAAQGLAVL
                     RQLGIADQDPRVNPNGGAIALGHPLGASGARLATTAISQLHATGGRYALCTMCIGVGQ
                     GIAVILERV"
BASE COUNT          388 a         1014 c         1084 g          488 t
ORIGIN      
        1 gctgcggcac acgggacgcc tgtcccgcaa aagcctgcga ctgatcctcc tgctgatcgg
       61 cgccgcgctg gtggccctgg tgtcgctggg ctttgcccgc atggccgatg ccgcgctcga
      121 atggaaccgg cgctggacga gcgcgcatgg ctggctggcc ttcatcgtgc tgccgctggg
      181 cctgttggcg ctgcgctggc tcacgctgcg cttcgcgcct tatgcgggcg gcagcggcat
      241 tccccaggtg atcggcgcgc tgtccctgcc gcccggccgg ccgcaatggc gtctggtgtc
      301 catgcagacg gcctggaaag tgccgctgac cttcctgggc atgctgatgg gcgcctccgt
      361 cggccgcgag gggccgtcgg tgcaggtggg cgccgccgtc atgctggcct ggagccggct
      421 gtgcgtgcgc ctgggggtgc cgctgcgcgg cctggacctg cgcgagctga tcgccgcggg
      481 ggcggcgggc ggcctggctg ccgccttcaa tgcgccgctg gcgggcgtgg tgttcgccat
      541 cgaggaactg gggcgcggca ttgcgctgcg ctggcagcgg cgcgtgctga tcgccgtgct
      601 gacggcgggc ttcctggtgg tggccgtggc cggcaacaac ccctatttcg gccatttcgg
      661 cggcgcggcg ttcgcgcacg gcatgctggc ctggacgctg ctgtgctgcc tggtcaacgg
      721 tgtgctgggc ggcgtcttcg cccgcctgct ggagaagggc ctggcgggca gcctgccgga
      781 ggcgctgggc gctgccgtgc gccgccatcc ctactggctg gccgccggca tggggctgct
      841 gatggcctgc ctgggcatgc tcacggccgg cgccgtgtac ggcacgggct atgccgcggc
      901 ggctggcatg ctggccggcg aaccggcgcc ggtggcggga ttcgggctgg ccaagctgat
      961 cgcgaccgtg gcctcgtact gggccggcat tccggggggc atcttcacgc cggccctgac
     1021 cacgggggcg ggtatcggcc atcagatctg gcactgggcc ggcgccggcg tggaccagcg
     1081 cgtgctggtg ctgctgtcca tggcggcttt cctggcggcg gccacgcagg cgccgctgac
     1141 ggccagcgtg gtcgtcatgg aaatgacggg cagccagccc atgctgttct ggctgctggc
     1201 ggcatcgcta ggcgcctcgg tggtgtcgcg gcagttcagc ccgcagccgt tttatcatct
     1261 gatggcaggc cgcttccggc ggcagacgct ggcggcggag gcgttcgcct cgaaaccggc
     1321 ctgaggctcg ctgcggcctc gccggcacgg taagatcgtg gcgttgagcc cctcggggat
     1381 gagacaccct gtattcctac acaagagaca ccacatcatg accacgcatg ctttcatctg
     1441 tgacgccatc cgcactccct tcggccgcta tggcggcgcc ctgtcctcgg tgcgtgccga
     1501 cgatctggcc gccatccccc tgaaggccct gatggcccgc aacccgggcg tcaaatggga
     1561 agaactcgac gacgtgctgt tcggctgcgc caaccaggcc ggcgaagaca accgcaacgt
     1621 cgcccgcatg gcgctgctgc tggcgggcct gcccatcgaa gtgccgggcg ccaccatcaa
     1681 ccgcctgtgc ggttcgggcc tggacgccat gggcaccgcc gcgcgcgcca tcgccgccgg
     1741 ccaggccagc ctgatgctgg ccggcggcgt cgagagcatg agccgcgcgc ccttcgtcat
     1801 gggtaaggcc gacacgcctt tctcgcgcaa tgccgccatc tacgacacca cgatcggctg
     1861 gcgtttcgtc aacaagctga tgaaggccca gtacggcgtc gattccatgc ccgagacggc
     1921 cgagaacgtc gccaccgact acggcgtcag ccgcgaggac caggacaagt tcgccctggc
     1981 cagccaggaa aagaccgtgc gcgcccagca gtcgggcttt ttcgacgccg agatcacgcc
     2041 ggtgagcatc gcccagaaaa agggcgatcc catccaggtc gtcaaggacg agcatccgcg
     2101 ccagaccagc ctcgaggccc tgggcaagct caagcccgtg gtgcgcgaag gcggcacggt
     2161 cacggcgggc aatgcctcgg gcgtgaacga tggttcggcc gcgctgctgc tggccgacga
     2221 aaagggcgcc gcgcgccacg gcctgaagcc gcgcgcccgc gtggtgggca tggccacggc
     2281 cggcgtcgcg ccgcgcatca tgggcatggg tccggtgccg gccacccgca aggtgctggc
     2341 ccagacgggc ctgacgatcg accagctcga tgtcatcgaa ctgaacgagg cctttgccgc
     2401 ccagggtttg gcggtgctgc gccagttggg cattgccgac caggatccgc gcgtcaatcc
     2461 caatggcggc gccatcgcgc tgggccatcc cctgggcgcc agcggcgccc gcctggccac
     2521 cacggcgatc agccagctgc acgccaccgg cggccgctac gcgctgtgca ccatgtgcat
     2581 cggcgtgggg caggggattg ccgtgattct ggaacgggtc tgacgcgagt cgaggtcttg
     2641 ggccgctggt ggcatttcaa gctgctggcg gttttcaagg gttcttgaac cgcactgccg
     2701 tcccggatag gggcgccgtc tgcatcgcgg cggctcgggc gcaagacgac gcgccctcgg
     2761 cccttcgggc ttcccgccgc tcaacgcagg cggagcccct atccgggacg gccgggctca
     2821 aagtgtctta agcatgcagg ctcgtttgcg taggccggtt gtcgggcctt gtggaattca
     2881 caggtatcta gtccgtcggg ggccggggct gggtgcgggc tgcgttgagc ggcgggcagc
     2941 cccgcagggg ccgagggcgc gtcgtcttgc gccc
//
LOCUS       DASWPO010000029        28390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2952609, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000029 DASWPO010000000
VERSION     DASWPO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 28390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28390
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2952609"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="VGC69_01400"
     CDS             complement(<1..1912)
                     /locus_tag="VGC69_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY0293969.1"
                     /translation="MPPVSSSSQSRHAGPPAAPIDARRIGLPPAQGLYNPDNEHDACG
                     VGFVAHIKGHKSHTIIQQGLKILENLDHRGAVGADKLMGDGAGILIQIPDTLYREDMA
                     QRGITLPQPGEYGVAMVFLPKETASRLACEQELERSVRAEGQIVLGWRDVPVDTEMPM
                     SPAVRSVEPVIRQLFIGRGPDVMVPDALERKLYVIRKTASHAILAMELAHGKEYFVPS
                     ASVRTVVYKGLLLADQVGRYYRDLADPRTVSALALVHQRFSTNTFPAWPLAHPYRMIA
                     HNGEINTVKGNFNWLRAREGMMQSAVLGEDLKKLYPIVYEGQSDTATFDNCLELLVNS
                     GYSLAHAMMMMIPEAWEQHTQMDESRRAFYEYHAAMMEPWDGPAAIAFTDGRQIGATL
                     DRNGLRPARYIVTDDDMVILASEAGTLPIPEHRITKKWRLQPGKMFLIDLEQGRIIDD
                     AEVKSQLANSRPYRQWIERLQIKLDSLPAPQNATSPATQTSVSLLDRQQAFGWTQEDY
                     KFILEPMAATGEEIIGSMGNDAPLAVLSNRAKPFYNYFRQLFAQVTNPPIDPIREQMV
                     MSLVSFIGPKPNLLDINNVNPPLRLEVAQPVLDGAAMAQIRDIEQVTGKKFRSQELDI
                     TYPAAWGREGIEA"
     gene            2272..2964
                     /locus_tag="VGC69_01405"
     CDS             2272..2964
                     /locus_tag="VGC69_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY0293970.1"
                     /translation="MSWQQNVYRLLENHFLTYRELASVPVEDCGQAMVQLGAGFSGKV
                     LSVDDMMKQYTGERVYVRAGLVERLEKAQQALKTVMPDCDLQVVYGYRHLDIQTFKFE
                     RHMNKVKRENPALSGDELYEVVHHFSAVPAVAGHPTGGAVDLHIVDRDGAPLPQGTPI
                     HQYADDTYVFSPFISKADARNRQILRYCMQSAGFAPFDGEWWHFSYGDREWACYYSQP
                     KAIYEQLRFTDA"
     gene            complement(3000..3590)
                     /locus_tag="VGC69_01410"
     CDS             complement(3000..3590)
                     /locus_tag="VGC69_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293971.1"
                     /translation="MPVISILLAFAASSIDDFILLVCLYADRRAGIGEITGAKMICVL
                     LALGAAIACATTWLALPLSLSRAMGLAPLALGIKRLIDHRRPRAEAGAGPPGAYAHMG
                     TGTLAQRTMRYVLTLFAASLDNVVLYLPLFAQGHQTAIVSTIGIVLALTLSLCGIALL
                     CSRIPIPFHAACLKLGAAVPYLMMYIGIKALAASWL"
     gene            complement(3651..4562)
                     /locus_tag="VGC69_01415"
     CDS             complement(3651..4562)
                     /locus_tag="VGC69_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0293972.1"
                     /translation="MFAPPAPIQTRVFARLPDSLRRSGQASAWTAARSHRDVHSFLEG
                     PSFDREGNLYCTDIPFGRIFRVSPDGQFTVVAEYDGEPNGLKIHRDGRLFVADHRRGL
                     LAIDPASGRIETVLDRAYGEGFRGLNDLIFASNGDLYFTDQGQSGMQDLSGRLYRLRA
                     GGELECVLDGMPSPNGLALSADESTVYVNLTRGNAVWKAPLDKHGRASKVGVFLHLNG
                     GTGPDGLAVDESGNLAIAHPGMGSVWVFSARGEPLYRILSCAGERPTNLAFGGPDNRT
                     LFITESESGSILCADLPAPGLRLYSHQ"
     gene            complement(4626..5612)
                     /locus_tag="VGC69_01420"
     CDS             complement(4626..5612)
                     /locus_tag="VGC69_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0293973.1"
                     /translation="MKSKAIHVIAALLLIICWNVSYAQWTPNGRVELIVAAGSGGGND
                     RMARVLAKLLQTSGLVPASIIVVNKPGAGGVVAQDYLNSHRGDGRYLMVTNPALITNP
                     VTGIGTAKYTQITPVAQLCTEYVTLVTSADSPFKTGADVIARLKRDPGSLSIAVAPGL
                     GAGTQIAISLVVKAAGIDPAKLRIVPYTSSGQAMTDLLGSHVDLLSTTPMNVLPQFEA
                     GKVRVLGVAAAQPLDGDLGRAPTWRAQGVDAVFGNWRGVVGPDGLKAPELAYWDGVFK
                     KLDALPEWRKELSLEIAQSQYMDSRTSKTFLDDQNNSLTQVLTSVGLAKPAD"
     gene            5795..6568
                     /locus_tag="VGC69_01425"
     CDS             5795..6568
                     /locus_tag="VGC69_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0293974.1"
                     /translation="MPTGTQSIRRSVAILREVATYNLNGIRLVDLAANLGLERSTAHR
                     ILQCLVYEGLIHEKNPGNRYVLGPLAFELGLAARRRFDVTSIFLPHMQSLADETGDVV
                     FLSVRSSLAITCIARVDGTYPIKAYTLDVGQRRPMGFGCVGTAMLTLMSDEEVHSILA
                     MNAQALQTYGATTVAEAFERVTRARELGYALHARPTLGLKSIAVPVADAAGKPAAVLS
                     ICAIARRLEGARLETLAESLQRYGAAAAGDFSAHRQSDA"
     gene            6796..8229
                     /locus_tag="VGC69_01430"
     CDS             6796..8229
                     /locus_tag="VGC69_01430"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY0293975.1"
                     /translation="MIQLSALGADGRREVRVLSASSVCGAGFLESSLEAALALNPTFI
                     GCDAGSTDPGPTYLGSGATAFPRSAIKRDLRLMLLGARRLGIALLIGSAGTAGADSQV
                     ELMREIVLEIAREEGLGFTLALIRSEVSKPWLLERLHAGRVHALDPAPPFDESVVERS
                     AHIVGMMGAEPFMQAVRAGADVVIAGRASDTAIFAAIPLMLGIPPGIAWHAAKILECG
                     AASVVRRKTPDCMFAWLRDDHFVVQAPDPDLRCTPQSIASHSLYENADPFRLVECSGT
                     LDLTDSIYEAESERAVRVSGSTFIEAPRYTVKLEGAEKAGYQSVLLGSVRDPFIIGQI
                     DDWLARLMERIHARVHMVYGDTLGADDYLLHVRVYGKDGTMGPLEPVKEIRSHELCLV
                     FEATGPTQEIATALAGIVRHQALHLSIPQWSGLITSLACPYNALTRGEVYRFNMNQVV
                     ELDDPCEIFPLELVDVEPAQSRRSTAR"
     gene            8226..8534
                     /locus_tag="VGC69_01435"
     CDS             8226..8534
                     /locus_tag="VGC69_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEY0293976.1"
                     /translation="MTRLADLARLIRSKNAGPFQLTFDIMFDDAPTYERVKRAGSLSR
                     ASVARLYGLSEADTLFFHCDNALAIKVSIPRPYIQGDPLDSDGHGGQQYAPLMDIEIP
                     "
     gene            complement(8608..9399)
                     /locus_tag="VGC69_01440"
     CDS             complement(8608..9399)
                     /locus_tag="VGC69_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0293977.1"
                     /translation="MSDATIIKVTDLAKRFGAVEVLKGVSLALPRSQVTCIIGPSGSG
                     KSTLLRCMAFLETYDSGRIEIEGRLLGYVENQGVRRPATPREVSDVRRNVGFVFQHFN
                     LWPHMTALENVAKPLWLAGGFSKPDAKERATAALAKVGLADKLDSYPIQLSGGQQQRV
                     AIARTLAKEPHIILFDEPTSALDPEIVGEVLQVMRELADDGMTMAVVTHEMGFAAHVA
                     NRIAFIDQGIIAMEGPPKEVLLNKDNPRLARFLKNFFDRNNFWEG"
     gene            complement(9421..10086)
                     /locus_tag="VGC69_01445"
     CDS             complement(9421..10086)
                     /locus_tag="VGC69_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0293978.1"
                     /translation="MSMWDFSGFDGRWAEILAGLVLTLELWVAGTALGLLIGLALAAI
                     QVNAGARTRAIVRGYVAIFRGTPLLVQLFLLYYGGPSVGLLLTPAAAGLLGLAIYASA
                     QFIEIFRSGFESVPRGQIEAARISGLSRGQILAHVELPQMLIIIVPSLINTLVIMTKE
                     TAILSAISIPDLTAVLYGIGSETYNIIPPLFCLAIFYWALLECVTVLGRGLERRASRY
                     LNH"
     gene            complement(10090..10734)
                     /locus_tag="VGC69_01450"
     CDS             complement(10090..10734)
                     /locus_tag="VGC69_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0293979.1"
                     /translation="MAFLHEVLPILWAGLWSTVLYALGCVAVGFPIAVLLCVLRLSRM
                     RLLSGCATVYISFFRGVPLLVQLLIAFYAVPALGVDVPPWAAGLFTLALCTAAYMAEI
                     LRGGFQAIPYGQVEAAQISGLNPRQILFFIQVPQAVRLTLPSLINEAITMVKASSLIS
                     IVGVLDLTRASQNMASSTLRPLACYALAGLLYLAVTSCVAAAGHRLERTLAAGR"
     gene            complement(10749..11573)
                     /locus_tag="VGC69_01455"
     CDS             complement(10749..11573)
                     /locus_tag="VGC69_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0293980.1"
                     /translation="MRHLKTLAVACAALLSFALAGAAHAQDALARAKAKGVLVIATEE
                     QFPPFDFIKDGKHVGFNVDYFEEVGKKMGLKLEWIDLPWASVLPGLQAKKYDLVAGPA
                     SITVERQKVLRFLPPITSVEAMIQIRKGEKGISSLADIAGKKIGAERASYADNEVKKF
                     GATLPNPPTIIEYQDTAQAAADLEAGRLDGVANSSSNVKDSVKRRPDAFDLIPTGFGL
                     KTYSGFMIRKDDDSKTLFDAVSATMLDIKKEGKLTELQKQWLGEAVEVPDAAPELK"
     gene            complement(11778..12515)
                     /locus_tag="VGC69_01460"
     CDS             complement(11778..12515)
                     /locus_tag="VGC69_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0293981.1"
                     /translation="MARLPRLYAPGLPQLVQASFVQPLAAPGQPAPQDILNQLSAWLG
                     DSAQRHRVAVHSWVLATDRILLLATPADEAGLPRLMQTLGRNLAARLRGGRVFAGRYR
                     SALLEPHAWVLRAQIWLETYPARSGNASDSDSWPWSSAGSHTGASHQTPVQWQTDHAD
                     YWACGNTPFDRQANYRKLLQDGLSREESLRIEQAVSGQWALGGPAFIASLAHTASRRV
                     SPGQRGRPRKKPLSAVSAPPESGPATP"
     gene            12820..13707
                     /gene="rpoH"
                     /locus_tag="VGC69_01465"
     CDS             12820..13707
                     /gene="rpoH"
                     /locus_tag="VGC69_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY0293982.1"
                     /translation="MKQPSMTSAESASALALAVANPGALGTIDAYISTINRLPVLSAE
                     RETDLARRLRDHDDLEAARELILSHLRLVVSVARQYLGYGLPHADLIQEGNVGLMKAV
                     KRFDPERGVRLVSFAVHWIKAEIHEYIVRNWRMVKVATTKAQRKLFFNLRSMRPDGQT
                     LDPEQVEHIARELNVRREDVSEMEVRLSGRDLSLEGQDDDEDSFAPIAYLSDEGREEP
                     TRVLARQARATLQGEGLNQALEGLDPRSRRIIEARWLQDDGGLTLHDLAAEFSVSAER
                     IRQIEAAALKKMRGALSPA"
     gene            13820..16354
                     /locus_tag="VGC69_01470"
     CDS             13820..16354
                     /locus_tag="VGC69_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0293983.1"
                     /translation="MDVGLIVFGIAGLLTLVCFMPPLAGRLKLPYSVLLAIVGCLLGI
                     LMHWHGWVPPVLDDLIVTLDRFDISSDTFLTVFLPVLLFETSLSMNARRLMEDIGPIL
                     MMAIVAVVVCTIVVGLALSAASSYGIIVCLLLGAIVATTDPVAVVGIFREVGAPKRLT
                     TLVEGESLLNDAASIALYSVLVAALASGAAGAGQHLSAGGVLRDFLYTFLGGGLAGYA
                     MGRVACWLFALLRGFPTAEITLTLTLAYLSFFISEHYLGVSGVVATVIAGLVVGSTGR
                     TRMSPTTFEHLAVAWEQFGFWANSLIFLFASMLIPRMMANAGWREILLVVLVFAATLV
                     ARAVVVFGLLPLLRLMRLGTRVSNSYKAVMLWGGLRGAVSLALALAVTEQEAIPAPVR
                     QFVGVATTGFVLMTLFINGTTLRSLIRVLGLNQLSSMERALRNQALTIALEDLQERTD
                     EIARTDHIGGEVSGRIHAVFDASLASVHDAQTAQLTVDQRVLVGLATVALREQEMFFD
                     ILKAQIVDWRMAETLLARAERLEDAIRSHGLPGLEAAIEADVRYGRSFRMALRAYDLF
                     GWQGWLARELAQRFSNLMTKRTVAQQLIKFTRGELASLLGEEVAQRIAEVHQRRLGLI
                     ENALQALNLQYPSYAQWLQESYLGRVARELERRRYRTMLDQFLISGEVYADLMNQLKS
                     RWQHIDRHPQLDMEMGAADLITRVPLFEGLSSESLRAIGRLLRPRLTLPDQAILEGGG
                     YGEAMCFVASGAVAVHLPDGTAVELGSGEFFGELALLGELQVKPKVTSLGYSRLLMLS
                     ARDFHALLAKDADLRGRIEVVAKQRMRAIEVWKQFSQQPSPDGAAT"
     gene            complement(16376..17173)
                     /locus_tag="VGC69_01475"
     CDS             complement(16376..17173)
                     /locus_tag="VGC69_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEY0293984.1"
                     /translation="MHRIPLARLAPLYDALRARASEPPPALALPLVLAGRRCGWITQA
                     ARAALHAAGLAATEGDAVQLLPGLAPGPRLDASLAQVAQVLREARCLRGWRNELLDIH
                     ADGAHLGAIERAAMRPLGLLTYAVHLNAWTPDGRLWVARRALSKATDPGLWDTLVGGL
                     AGSNEPLDLALERECGEEAGLDAPHLRGRGPLRTILRLHRRLPEGYQVEDLLVSRCVL
                     APDAQPVNRDGEVMEIAAMPIDDVIGRIEAGEFTLEAALVIVEALMS"
     gene            17258..18292
                     /locus_tag="VGC69_01480"
     CDS             17258..18292
                     /locus_tag="VGC69_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY0293985.1"
                     /translation="MDRITHRYRRLVFLTWFLTLDLIMFGAFVRLEDAGLGCPDWPGC
                     YGSASPVGSLADIHQAAHAMPYGPVSLSKAWIEMVHRYVGAALGLLIIAILYMAWRHR
                     RHLGRSPALATAALVAVCLQGAFGAWTVTHRLMPVVVTAHLAIGLIVLALMTWLAARE
                     KPHLALAAAAARLRPWAAAGLALLSVQILLGGWVSTNYAALACMDFPTCQGAWLPAMD
                     FSGGFSLIRALGELPSGEVISQEALTAIHWTHRNFAFVVFVYLAVLGWRLRAEAGLRG
                     PATLLLALLLAQVCTGLTTIFFQWPLAVAVLHTGGAAAMTLALVLILVRLGTAGGPPV
                     RADWTRAGAL"
     gene            18319..19218
                     /gene="cyoE"
                     /locus_tag="VGC69_01485"
     CDS             18319..19218
                     /gene="cyoE"
                     /locus_tag="VGC69_01485"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0293986.1"
                     /translation="MTAVTASSPTGLLRQYLVLTKPKVTQLAVFCAVIGMFLAVPGVP
                     RWDRVVYGVLGIWLLAAAAFAINCLIEQEVDARMTRTARRATAQGTISSFHVLGLSGL
                     LGGAGMLVLYWLVNPLTMWLTFATFVGYALIYTVLLKPYTSQNIVIGGLAGAMPPALG
                     WAAVANAVPAQAWVLVLIIFVWTPPHFWALALYREQDYAKSGLPMLPITHGRRYTQLQ
                     ILLYSLGLLAVTLLPYAMRTSGLPYLLCALVLGGLFVRHAWRLFQSYSDAQARRLFRY
                     SILYLSLLFAALLVDHWVGLLLA"
     gene            19226..19846
                     /locus_tag="VGC69_01490"
     CDS             19226..19846
                     /locus_tag="VGC69_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0293987.1"
                     /translation="MKPTLAGRRRVLRAAASGGLVLALPLALAACGDKGPVFKGSDVT
                     GTHLGRALALTDFNGQARTLASFAGKVLVVFFGYTQCPDVCPTSLAELADVMRKLGPD
                     ADRVQVLFISVDPERDTPEILKQYTTAFDPRFLALRGTADETRQTATSFKAYYAKVSS
                     KGGSADEYSMDHTAAFYLFDGKGEARVLASHSSSADDLAQDIKALL"
     gene            complement(19888..20106)
                     /locus_tag="VGC69_01495"
     CDS             complement(19888..20106)
                     /locus_tag="VGC69_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HEY0293988.1"
                     /translation="MERITLPQLEEAINYWRNRAPAQGEESRLCPEAAALASPYALMI
                     IGHRPEIGADELAPAARAAYQAWLTSVS"
     gene            20164..20904
                     /locus_tag="VGC69_01500"
     CDS             20164..20904
                     /locus_tag="VGC69_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEY0293989.1"
                     /translation="MSDSNNANPFVLPGMGQSSDLSGNPLLASMEMMRQAWAGLAGPG
                     GLAQMLPMASPMNTEELERRIAELRAVESWLRLNLSMLDSTIQGMEVQRATIATLRSF
                     VDKVSSMDGSSLAPGDPSPLEVALGINPASAKAPSGAEASGKPAGEEKAGESFNAAAQ
                     SASQAWWNLLQQQFNNIAAATTANMPGAQPGEQSAAGQPKAAAQPAVRPTTKARTTAS
                     KPAPRKKPAPRKKAAPRKKAAPSSDQSR"
     gene            20929..22302
                     /gene="glmU"
                     /locus_tag="VGC69_01505"
     CDS             20929..22302
                     /gene="glmU"
                     /locus_tag="VGC69_01505"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY0293990.1"
                     /translation="MLNVVILAAGLGKRMKSDLPKVLHILAGSPMLTHVLGSARQLAP
                     ARIVVVVGHGAEQVRAAYEGQPDLCFALQQPQQGTGHAVRQAVPQLLEGDGAGDVTLV
                     LYGDVPLVQPGTLERLLAARGQGVAVLTEVLQDAAGYGRIVRDAHGQVLRIVEHKDAN
                     EAELTIHEVNTGMLAAPTARLKDWLGRLGNDNAQGEYYLTDIIGLAVADGVPVKAAHP
                     DAQWETMGVNSRMQQVQLERAWQREQARRQLEAGVTLADAERFDVRGSLACGRDVFID
                     VGCVFEGRVELGDGVRVGPYSVLRNVSVQAGTRIEAYSHLDDARIGRDAHIGPYARLR
                     PGASLGDRVHIGNFVEVKNSVVGNDSKANHLAYVGDTDMGERVNVGAGTITCNYDGAN
                     KWRTVIEDDVFIGSDSQLVAPVRVGRGATLGAGTTLTRDAPPDQLTISRTRQLSIEGW
                     KRPAKKK"
     gene            22299..23681
                     /locus_tag="VGC69_01510"
     CDS             22299..23681
                     /locus_tag="VGC69_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0293991.1"
                     /translation="MTEAADTPRAAPQDGLPAPRRYWAAGTILCGMSLTVLDSTIVNV
                     ALPTIADDLGVPASRVVWVVNAYSLTIVMLLLLMSSLAERLGFKRMFAFGVVVFMLSS
                     LGCALSTTLWQLNFSRVCQGVGAATLMCMFGGMVRNIYPIRLLGRGISLNSTVVALMS
                     VLGPSIGSLILSVVSWRWIFAVNVPMSVLLLFGLRVLPDIPRTKGTLDWVSALVSMAA
                     LGTFIIGVDNLAENLPKALGLIVVAALLAVILMRRARGQTAPLVPVDLLRIKPLAYAV
                     AASVFTFAAQTTSYVALPFYFLHTLDRPQLQLGMLMGAWPIGTAAIAVIAGRLSDRYS
                     PALLSGLGAGAMAIGLLWIALLPQDASTASIIGGMFLSGMGFGFFQTPNNRALLGSAP
                     RQRSGAAGGLQATTRVFGQSFGTALVAVAYGLSLGHGSTIALILASLCAVLAVGVNTV
                     RSAKLAAIQP"
     gene            23705..24484
                     /locus_tag="VGC69_01515"
     CDS             23705..24484
                     /locus_tag="VGC69_01515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0293992.1"
                     /translation="MPADTPAPAAQPRLSVVIIACNESRHIGACLDSVAFADERIVLD
                     SGSSDDTCDIARARGAIVHATPDWPGFGPQKNRALALATGDWVLSLDADERVPPELAD
                     EIRAVLRAPQAQAYRMARLSEAMGRAIRHGGWWPDHVLRLFRRGTARFSDDAVHEKVV
                     AEGEVGLLRGHLLHYTYDSIDAMLAKMNRYSTDSAQMMLQRGKRATALGAIGHGVWTF
                     IRMYVLRRGFLDGWQGLAIASTGAAGSFFRYLKLLDLRRRN"
     gene            24692..25690
                     /locus_tag="VGC69_01520"
     CDS             24692..25690
                     /locus_tag="VGC69_01520"
                     /inference="COORDINATES: protein motif:HMM:NF005757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0293993.1"
                     /translation="MDHQLFECGDFALHGGGILRGARLAYKAVGTLNAARSNAIVYPT
                     SFGTTHADIEWLVGPGKALDPQRYFIIMPSMLGNGLSSSPSHGQPGTFPELSLWDSVR
                     LQHRLVAEHYGIQRLALVLGFSMGGQQAYHWATLFPDRVERLAVMCASARTSARNRVF
                     LGEIRAALTDDPAWNGRFFEARAQRGVRAMAQVYADWALPAEFYRRETFRAIGFQSRQ
                     DFVARDWEVFLGRGDPNDLMAMMGVWERADVGGLPAFGGDTSLALRSIRAAALIMPST
                     TDQYFLASGIEAEARMISGARYCPIETIWGHRVNNPVQNPADARFVDDNLRLLLAV"
     gene            complement(25698..26162)
                     /locus_tag="VGC69_01525"
     CDS             complement(25698..26162)
                     /locus_tag="VGC69_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0293994.1"
                     /translation="MPILDDLDRRILDQMQRDSALTNQELAARVHASPPTCLRRVRRL
                     VETGVIVRQVALLDPEKLGATLTAIVEVTLDVQTAEQMAAFEDAMQLEAAVLQCYRVS
                     TGPDFLVIAQVQDMPAYHALVHRAFTAQGNVRNVRTFFSVNRSKFETRIALA"
     gene            26335..28170
                     /gene="glmS"
                     /locus_tag="VGC69_01530"
     CDS             26335..28170
                     /gene="glmS"
                     /locus_tag="VGC69_01530"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY0293995.1"
                     /translation="MCGIVGAVAQRDITPILIEGLRRLEYRGYDSCGVALYVDGHLRR
                     ARTTQRVAELADEVAKEKLAGYTGIAHTRWATHGGPSVYNAHPHFSAQGKDEPRIGLV
                     HNGIIENYEELRAELIAAGFVFESQTDTEVVAHLINHLYQGDLFEAVQQAARRLKGAY
                     ALAVFCRDEPHRVVGARHGSPLVVGLGKNENFLASDALALAGTTDQIIYLEDGDVVDL
                     QLSKVWIVDAAGKRIERKVHTVQIHAGQAELGPYRHYMQKEIFEQPRAVGDTLQVVES
                     IATTLFGARAHDVLPKINSLLILACGTSYYAGLTAKYWIESLAKIPVNVEIASEYRYR
                     DSVPDPKTLVVTITQSGETADTLAALKHAQSLGMTHTLTVCNVATSAMVRECELNYIT
                     HAGVEIGVASTKAFTTQLTALFLLALTLAKLRGRLSPEDEALHLKNLRHLPVAISAVL
                     ALEPQIIAWADRFATKENALFLGRGMHYPIALEGALKLKEISYIHAEAYPAGELKHGP
                     LALVTDQMPVVTIAPNDALLEKLKSNMQEVRARGGELYVFADADSKIIAEDDGMHVIR
                     MPEHYGMLSPILHTVPLQLLAYHTAIARGTDVDKPRNLAKSVTVE"
BASE COUNT         4545 a         9348 c         9630 g         4867 t
ORIGIN      
        1 gggcttcgat gccttcgcgg ccccaggcgg ccggataggt gatgtccagt tcctggctgc
       61 ggaatttctt gccggtgacc tgttcgatgt cgcggatctg cgccatggcg gcgccgtcca
      121 gcaccggctg cgccacttcc agacgcagcg gcgggttgac gttgttgatg tccagcagat
      181 tgggcttggg gccgatgaag gacaccagcg acatcaccat ctgttcgcgg atcggatcga
      241 ttggcgggtt ggtgacctgc gcgaagagct ggcggaagta gttgtagaag ggcttggcgc
      301 ggttcgacag cacggccagc ggggcatcgt tgcccatgga gccgatgatt tcctcgcccg
      361 tggcggccat gggctcgagg atgaacttgt agtcttcctg cgtccagccg aaggcctgct
      421 ggcggtcgag cagcgagacg ctggtctgcg tggcgggcga ggtggcgttc tggggcgcgg
      481 gcagcgagtc gagcttgatc tgcaggcgct cgatccactg gcggtagggg cggctgttgg
      541 ccagctgcga cttgacctcg gcgtcgtcga tgatgcggcc ctgctccagg tcgatcagga
      601 acatcttgcc gggctgcagg cgccacttct tggtgatgcg gtgctcgggg atgggcaggg
      661 tgccggcctc ggacgccagg atgaccatgt cgtcgtcggt gacgatgtag cgggccgggc
      721 gcaggccgtt gcggtcgagc gtggcgccga tctggcggcc gtcggtgaac gcaatggccg
      781 cggggccgtc ccagggctcc atcatggccg cgtggtactc gtagaaggcg cggcggcttt
      841 cgtccatctg ggtatgctgc tcccacgcct cggggatcat catcatcatg gcgtgagcca
      901 gcgaatagcc ggagttgacc agcagttcga ggcagttgtc gaacgttgcg gtgtcggact
      961 ggccttcgta gacgatggga tagagcttct tgaggtcctc gcccagcacg gccgactgca
     1021 tcatgccctc gcgggcgcgc agccagttga agttaccctt gacggtgttg atctcgccgt
     1081 tgtgggcgat catgcggtag gggtgggcca gcggccaggc cgggaaggtg ttggtcgaga
     1141 agcgctggtg caccagcgcc agggccgaga cggtgcgcgg gtcggccagg tcgcggtagt
     1201 agcggccgac ctggtcggcc agcagcaggc ccttgtagac cacggtgcgc accgaggccg
     1261 agggcacgaa gtattccttg ccgtgggcca gctccatggc caggatggcg tggctggcgg
     1321 tcttgcggat gacgtagagc ttgcgttcca gcgcgtcggg caccatgacg tcggggccgc
     1381 ggccgatgaa cagctggcgg atgacgggct cgaccgagcg cacggccggc gacatgggca
     1441 tctcggtgtc gacgggcacg tcgcgccagc ccagcacgat ctggccctcg gcgcgcaccg
     1501 agcgttcgag ctcctgctcg caggccaggc gcgaggcggt ttccttgggc aggaagacca
     1561 tggccacgcc gtattcgccg ggctggggca gggtgatgcc gcgctgcgcc atgtcctcgc
     1621 ggtagagcgt gtcggggatc tggatgagga tgcccgcgcc gtcgcccatg agcttgtcgg
     1681 cgccgaccgc gccgcggtga tccaggttct cgaggatctt caggccctgc tgaatgatgg
     1741 tgtggctctt gtgccccttg atatgggcga cgaaacccac gccgcaggcg tcatgctcgt
     1801 tgtcggggtt gtacaggccc tgggccggcg gcaggccgat gcggcgcgcg tcgatggggg
     1861 ccgccggcgg gccggcgtga cgggactggg aggaggacga aacagggggc atggcgcgct
     1921 ccatgggaga ctgcgggaat gttgctatgg attgaacggc gaagatagcg cgccgcagca
     1981 tgcaatgcaa gcgcaacaat aagggtgtgt ccctgtattt aaatgtcgaa caccttttat
     2041 ttataaaaat agggacgtta ttttttcaat ataaaacata gacttgtaaa ttcacattgc
     2101 ccatggacat gacaagggga ttgcggattt tctatattgc gtccccgttt aattaaagcc
     2161 ggatcggacg cggcggaagg tctgttcccc gccatgtccg gccagtccgg cgcctttcgg
     2221 ccgggctggc caggacgtat catcccggta ttcaaacgga gcgcgtcaac catgtcctgg
     2281 cagcaaaatg tctaccgcct gctcgaaaac catttcctga cctaccgcga gcttgcctcg
     2341 gtgcccgtcg aggactgcgg ccaagccatg gtgcagctcg gcgcgggctt ttccggcaag
     2401 gtgctgtccg tcgacgacat gatgaagcag tacaccggcg agcgcgtcta cgtccgcgcc
     2461 gggctggtcg agcggctgga aaaagcgcag caggccctca agacggtgat gcccgactgc
     2521 gacctgcagg tggtctacgg ctaccgccac ctggacatcc agaccttcaa gttcgagcgg
     2581 cacatgaaca aggtgaagcg ggaaaacccc gcgctttccg gcgacgagct ctacgaggtg
     2641 gtgcaccact tcagcgccgt gccggcggtg gccggccatc ccaccggggg cgccgtcgac
     2701 ctgcacatcg tcgaccgcga cggcgcgccg ctgccgcagg gcacgcccat ccaccagtac
     2761 gccgacgaca cctacgtctt ctcgcccttc atcagcaagg ccgacgcgcg caaccgccag
     2821 atcctgcgct attgcatgca gtcggcgggt tttgcgccgt tcgacgggga gtggtggcac
     2881 ttctcgtacg gcgaccgcga atgggcctgc tattacagcc agcccaaggc catctacgag
     2941 cagctgcgct ttaccgacgc gtagaaaaca tggcggccgc cggcgcgcgc gaggcgcttt
     3001 caaagccatg acgccgccag cgccttgatg ccgatataca tcatcagata cgggacggct
     3061 gcgccgagct tcaggcacgc ggcgtgaaag gggatcggta tccggctgca cagcagggcg
     3121 attccacaga gcgacaaggt cagcgcaagg acgatgccga tcgtggacac gatggcggtc
     3181 tggtgcccct gtgcgaacag cggcaggtag agcacgacat tatcgaggct ggctgcgaag
     3241 agggtaagca catagcgcat ggtgcgctgc gccagcgtgc ctgtgcccat gtgcgcgtac
     3301 gcgccaggcg gcccggcgcc ggcctcggcc cgcggcctgc gatggtcgat aagccgcttt
     3361 atgcccaatg ccagcggagc gaggcccatg gccctcgaca aggaaagagg cagcgcaagc
     3421 caggtcgtgg cgcaggcgat tgccgcgccg agcgccagca acacgcaaat catctttgcg
     3481 cccgtgattt cgccaatgcc ggcacgccgg tcggcataga ggcagaccag cagaatgaag
     3541 tcgtcaatgc tcgacgcggc gaaagccagc agaatggata tcaccggcat tgccctggcc
     3601 tacgcccacg agaagcccgg acgggtcgcc acggtgccgg ccggggtcgg ctactggtgt
     3661 gaatagagcc gcaggccggg cgcgggcagg tcggcgcaaa gaatgcttcc gctttccgat
     3721 tcggtgatga acagcgtgcg gttgtccgga ccgccgaagg cgagattggt cggacgctcg
     3781 cccgcgcacg agaggatgcg gtatagcggt tcgccgcgcg cgctgaaaac ccagacgctg
     3841 cccatgccgg gatgggcgat ggcaaggttg ccgctttcgt ccaccgcgag gccatccggt
     3901 ccggtcccgc cattcaggtg caggaacaca ccgaccttcg aggcacggcc gtgcttgtcc
     3961 agcggcgcct tccacacggc gttgccgcgc gtcagattga cgtatacggt ggactcgtcg
     4021 gccgacagcg ccaggccgtt ggggctgggc atgccgtcca ggacgcattc cagctcgccg
     4081 ccggcgcgca ggcggtacag ccgcccggaa aggtcctgca tgccgctctg gccttggtcg
     4141 gtgaaataga gatcgccatt gctggcgaaa atcaggtcgt tcagtccacg gaacccttcg
     4201 ccataggcgc ggtccagcac agtctcgatt cggccgctgg ccggatcgat ggcaagcagg
     4261 ccgcgccgat ggtcggcgac gaacagtcgg ccgtcgcgat ggatcttcag gccgttgggc
     4321 tcaccgtcgt actcggcgac gacggtgaat tgcccgtcgg gagatacacg gaaaatgcgg
     4381 ccgaaaggga tgtccgtgca atagagattg ccttcgcggt cgaatgacgg cccttccagg
     4441 aaagagtgca cgtcgcgatg cgaacgcgca gccgtccacg cgctggcctg gccgctgcgg
     4501 cgcagcgaat cgggcaggcg tgcgaagacc cgggtctgga tcggagcggg aggagcgaac
     4561 atggggaggt ttcctgtcag tggagagttg ggcgtggcgg gaccgatccg acgcacggcg
     4621 cgggctcagt cggcgggctt ggccaggcct accgaggtca gaacctgcgt caggctgttg
     4681 ttctgatcgt cgagaaaggt tttgctggtg cggctgtcca tgtattgcga ctgggcaatt
     4741 tccaggctca gctccttgcg ccattcgggc aaggcatcga gcttcttgaa taccccatcc
     4801 caataagcca gttccggcgc cttcaggcca tccggcccca cgaccccgcg ccagttgccg
     4861 aataccgcat ccacgccctg ggcacgccag gtcggggcgc ggcccaaatc gccgtccagc
     4921 ggctgggccg cggccacgcc cagcacgcgc accttgccgg cctcgaactg gggcaggaca
     4981 ttcatcggcg tagtcgatag caggtcgacg tggctaccca gcaaatccgt catggcctgc
     5041 cccgagctgg tgtagggaac gatgcgcagc tttgccggat cgatgccggc ggccttgacc
     5101 accagggaga tggcgatctg ggtgcctgct cccaagccgg gcgccacggc aatgctcagc
     5161 gagccggggt cgcgcttgag gcgggcgatg acatccgcgc cggtcttgaa cggagaatcc
     5221 gcgctggtga ccagcgtgac gtattccgtg cacagctggg ccacgggcgt aatctgcgtg
     5281 tactttgccg taccgatacc ggtgaccggg ttggtgatca atgccggatt ggtcaccatc
     5341 aggtaacggc cgtcgccgcg atgcgaattc aagtagtcct gcgcgacgac gcctccggcg
     5401 ccaggcttgt tcaccacgat gatggatgcg ggaaccaggc ccgaggtctg caacagtttg
     5461 gccaatacgc gcgccattct gtcgttgccg cctccggagc cggcggcgac gatcagttct
     5521 accctgccgt tcggcgtcca ttgcgcatag gacacgttcc agcaaataat caacaatagt
     5581 gcagcgatga catggatcgc cttgcttttc atctcctcac ctcttttgcg aaatgctcgg
     5641 ccaggcggcc gcaatatgcg tggcatgtgc gtggaattcg cggggtagta taggtaggtg
     5701 ggtgcgcgaa aagacgcgcc gcgctgcggg aatatccaca atatgggaga cagcaataat
     5761 gatgagtcca gacgtctgcg tatcggatgc tttcgtgccg acgggcacgc agagcattcg
     5821 ccgctcggta gcgattctgc gcgaagtggc gacctacaac ctcaatggca tacggctggt
     5881 ggatctggcc gccaacctgg ggctggagcg ttccaccgcg catcgcatcc tgcaatgcct
     5941 ggtatacgaa gggctgatac acgagaagaa tcccggcaac cgctatgtgc tcggcccgct
     6001 tgctttcgag ctgggtctgg cggcgaggcg gcgctttgat gtgaccagca ttttcctgcc
     6061 gcacatgcag tcgctggccg atgaaaccgg cgacgtcgtg ttcctgtcgg tacgcagctc
     6121 cctggccatc acctgcattg cgcgcgtcga cggcacctat ccgatcaagg cgtatacgct
     6181 ggatgtcggc cagcgccgac ccatgggctt cggctgcgtt ggcaccgcca tgctgaccct
     6241 catgtccgat gaagaagtgc atagcatcct ggccatgaat gcccaagcct tgcagaccta
     6301 tggcgccacg accgtggccg aggccttcga gcgcgtgacg cgcgcacgcg agctgggtta
     6361 tgcccttcac gcccgcccta ctctcggcct gaaatccatt gccgttcccg tcgccgatgc
     6421 ggccggcaag ccggctgccg tcctgtcgat ctgcgcgatc gcgcgtcgac tcgaaggcgc
     6481 gcggctggag actctggccg aaagcttgca acgctatggc gcggccgccg ccggggattt
     6541 cagcgcgcac cgccaaagtg acgcataaaa ccgaatccag cggcgcattc gacccaaagc
     6601 cgcagacgca cccggcgcgc cgggacgcaa gcccgccgtc gccggacacc ggtctcccat
     6661 aacgtgggag accggcgcgc cgcgaggacc gcacgcggcg gttgccttcc tatacttttc
     6721 tccgatcggt tcagtgccgt cgcgccggaa ggccgacgac acggccgcga gtttggtcat
     6781 tcattcggga gtcacatgat tcagctttcc gcccttggtg ccgacggccg gcgcgaggtt
     6841 cgcgtcctgt cagccagcag cgtctgcggc gccggctttc tcgagtcgtc gcttgaggcg
     6901 gcgcttgcgc tcaatcccac cttcattggc tgcgatgccg gctcgactga cccgggcccg
     6961 acctatctgg gctccggcgc gacggcattc ccgcgcagcg ccatcaaacg cgatcttcgg
     7021 ctgatgctgc tcggcgcgcg gcgcctgggc attgcgctgc tgattggatc ggccggcacg
     7081 gccggcgcgg attcacaagt tgaactgatg cgggagatcg tgcttgaaat cgctcgcgag
     7141 gaagggctcg gcttcacgtt ggcgctgata cgctcggagg tatccaagcc ctggctgctg
     7201 gaacggctgc atgccggccg cgtccatgcg ctggacccgg ctccgccttt cgacgagtcc
     7261 gtggtcgaac gcagcgcgca catcgtgggc atgatgggtg ccgagccttt catgcaggcc
     7321 gtgcgggctg gcgccgatgt ggtgatcgcc ggacgcgcca gcgatacggc gattttcgcc
     7381 gcgatcccgc tcatgctggg cattccgccc ggcatcgcct ggcatgccgc caagatactg
     7441 gaatgcggcg cggcctcggt ggtccgccgc aagacaccgg actgcatgtt cgcctggctg
     7501 cgcgacgatc atttcgtcgt gcaggcgccg gaccccgact tgcgctgcac gccgcaaagc
     7561 atcgcctcgc atagcctcta tgaaaatgcc gacccattcc ggcttgtgga atgttcgggc
     7621 accctggatt tgaccgactc gatttatgag gcggagtcgg aacgcgcggt gcgggtatcg
     7681 ggcagcactt tcatcgaggc gccgcgctac accgtcaaac tcgaaggcgc ggagaaggcg
     7741 ggttaccaga gcgtgcttct cggctctgtg cgcgacccgt tcatcattgg gcagatcgac
     7801 gattggctgg cccggcttat ggagcgcatc catgcgcgtg tgcacatggt ctacggcgat
     7861 acgctcggcg cggacgacta tctcctccat gtgcgcgtgt atggcaagga cggcaccatg
     7921 ggaccgctgg agccggtcaa ggaaatccgt tcgcatgagc tgtgcctggt attcgaagcg
     7981 accgggccga cgcaagaaat cgccacggcg ctggccggca tcgtgcgcca ccaggccttg
     8041 catttgtcca tcccgcagtg gagcggcctg atcacttcgc tcgcctgccc ttacaacgcg
     8101 ctcacacgcg gcgaagtcta tcgcttcaat atgaatcaag tcgtcgagct tgacgacccc
     8161 tgtgagattt tccccctgga actggtcgac gtcgagccgg cccaatcccg acggagcact
     8221 gcccgatgac ccgactcgcc gacctggccc gactgatacg cagcaagaat gccgggcctt
     8281 tccaactgac gttcgacatc atgttcgacg acgccccgac ttacgagcgg gtcaaacggg
     8341 ccgggtcgct gagccgcgca tcggtggccc gcctctacgg cttgagcgaa gccgacacct
     8401 tgttcttcca ctgcgataac gcgctggcca tcaaggtttc gattccgcgc ccctacatcc
     8461 agggcgaccc gctcgattcc gacggccacg gcggccagca atacgcgccg ctgatggata
     8521 ttgagattcc ctgatagcga aggggccggc cgcgtcggct gccccgggac gcacgagcac
     8581 gaccctattg catcgggcgc ggaaaactca gccttcccag aagttgttgc ggtcgaagaa
     8641 gttcttcagg aagcgggcca ggcgcggatt gtccttgttg agcaggactt ccttgggcgg
     8701 gccttccatc gcgatgatgc cctggtcgat gaaggcgatg cggttggcca cgtgggcggc
     8761 aaagcccatt tcgtgggtca ccaccgccat ggtcatgccg tcgtcggcca gctcgcgcat
     8821 cacctgcagc acttcgccga cgatttccgg gtcgagcgcc gaggtcggct cgtcgaacag
     8881 gatgatgtgc ggctccttgg ccagggtgcg ggcgatggcc acgcgctgct gctggccgcc
     8941 ggagagctgg atcgggtagc tgtcgagctt gtccgccagg ccgaccttgg ccagggcggc
     9001 cgtggcgcgc tccttggcgt ccggcttgga gaagccgccc gccagccaca ggggcttggc
     9061 cacgttttcc agcgccgtca tgtgcggcca caggttgaag tgctggaaca cgaagcccac
     9121 gttgcggcgc acgtcggaga cctcgcgcgg cgtggccggg cggcgcacgc cctggttctc
     9181 gacgtagccc agtaggcggc cttcgatctc gatgcggccg ctgtcgtagg tttccaggaa
     9241 ggccatgcag cgcagcaggg tgctcttgcc cgacccggac gggccgatga tgcaggtcac
     9301 ctgcgagcgc ggcagcgcca gcgagacgcc cttgagcacc tccacggcgc cgaagcgctt
     9361 ggcgaggtcg gtgaccttga tgatggttgc gtcggacatg gcgtgtggct ggcggtggat
     9421 tcagtggttg aggtagcggc tggcgcggcg ctcgaggcca cggcccagca cggtcacgca
     9481 ttccagcagg gcccagtaga aaatcgccag acagaagagc ggcgggatga tgttgtaggt
     9541 ttccgagccg atgccataga gcacggcggt gaggtcgggt atggatatgg ccgacaggat
     9601 ggcggtttcc ttggtcatga tgaccagggt gttgatcagc gaaggcacga tgatgatgag
     9661 catctgcggc agctcgacgt gcgccagtat ctgcccccgc gacaggcccg agatgcgcgc
     9721 cgcttcgatc tggccgcgcg gcaccgactc gaagcccgag cggaatatct cgatgaactg
     9781 cgcgctggcg taaattgcca gccccagcag gcccgccgcc gcgggggtaa ggagcagacc
     9841 caccgaaggc ccgccgtagt agagcaggaa caactgcaca agcagcgggg tgccgcgaaa
     9901 gatcgccacg tagccgcgca cgatggcgcg cgtgcgcgcg ccggcgttga cctggatggc
     9961 ggccaatgcc aggccgatca gcaggcccag ggccgtgccc gccacccata gttccagcgt
    10021 cagcacgagc cctgccagga tttcggccca gcgtccgtcg aaaccggaaa aatcccacat
    10081 ggacatgatt cagcgtcccg ccgcgagcgt gcgttccagc cgatgccccg ccgccgccac
    10141 acagctggtg acggccaggt agagcaggcc ggccagcgca tagcaagcca gcggccgcag
    10201 cgtgctggag gccatgtttt gcgaggcgcg ggtcaggtcc aggacgccga ctatcgaaat
    10261 gagcgaggag gccttcacca tggtgatggc ctcgtttatc agcgagggca gggtcaggcg
    10321 cacggcttgc ggcacctgga tgaagaacag gatctgccgg gggttcagcc ccgatatctg
    10381 ggcggcttcg acctgtccgt acgggatggc ctggaagccg ccgcgcagga tttccgccat
    10441 gtaggcggcc gtgcagagcg ccagggtaaa tagccccgcc gcccaggggg gcacgtccac
    10501 gccgagggcg ggcaccgcat agaaggcgat cagcagctgc accagcaggg gcacgccgcg
    10561 gaaaaagctg atgtagaccg tggcgcagcc ggacagcaac cgcatccggg acaggcgcaa
    10621 gacgcagagc agcacggcga tcggaaaccc gaccgccacg cagcccagcg catacagcac
    10681 ggtagaccac agacctgccc agagtatggg caggacttca tgcagaaacg ccacgcgtct
    10741 cggccttgct acttcagttc aggcgccgcg tcgggcacct cgacggcctc gccgagccat
    10801 tgcttctgca gttcggtgag cttgccttcc ttcttgatgt ccagcatggt cgccgagacg
    10861 gcgtcgaaca gcgtcttgga gtcgtcgtcc ttgcggatca tgaagccgga gtaggtcttg
    10921 aggccgaatc cggtcgggat caggtcgaag gcatccgggc gccgcttgac cgagtccttc
    10981 acgttggagc tggagttggc cacgccgtcg aggcggccgg cttccaggtc ggcggctgcc
    11041 tgggcggtgt cctggtattc gatgatcgtg ggcggattgg gcagggtggc gccgaacttc
    11101 ttgacctcgt tgtcggcata ggaggcgcgt tccgcgccga tcttcttgcc ggcgatgtcc
    11161 gcaaggctgc tgatgccctt ctcgcccttg cggatctgga tcatcgcctc gacgctggtg
    11221 atgggcggca ggaagcgcag gaccttctgg cgctcgacgg tgatcgaggc cgggccggcg
    11281 accaggtcgt atttcttggc ttgcaggccg ggcagcacgc tggcccaggg caggtctatc
    11341 cattcgagct tcaggcccat tttcttgccg acttcttcga agtagtcgac gttgaagccg
    11401 acatgcttgc cgtccttgat gaagtcgaag ggcgggaatt gctcttccgt ggcgatgacc
    11461 agcacgccct tggccttggc gcgggccagg gcatcctgcg cgtgggcggc gccggccagg
    11521 gcgaacgaga ggagggcggc gcaagcaacc gccagagtct tcagatgacg catggttcat
    11581 accccttggg atggtcggct ggctcggcga gggcgccccg ccagcctgct tgtataggca
    11641 cgtctgtaca gcggcagtga gtctgtataa aagtcatggg cccgtcaagc cggaagcgga
    11701 atcatcgcat aggttccccg gcccgcggcg ccggggaggc ggccgcaggc ctaggggaaa
    11761 gcctgggggt ggagaggcta gggcgtggcc gggccggatt ccgggggggc ggacacggcc
    11821 gacaggggct ttttgcgggg ccgtccgcgc tggccggggg agacgcggcg gctggcggta
    11881 tgggccaggc tggcgatgaa ggccggaccg cccagcgccc actggcccga gacggcctgc
    11941 tcgatgcgca gggactcttc gcgcgacagg ccgtcctgca ggagcttgcg gtagttggcc
    12001 tggcggtcga agggcgtgtt gccgcaggcc cagtagtcgg cgtggtcggt ctgccactgg
    12061 acgggcgtct ggtggctggc gccggtgtgg ctgccggccg aggaccaggg ccaggaatcg
    12121 gagtcggatg cgttgccgct gcgcgcgggg taggtctcga gccagatctg ggcgcgcagc
    12181 acccaggcat ggggttcgag cagggccgag cggtagcgcc cggcgaagac ccggccgccg
    12241 cgcaggcggg cggccaggtt gcggccgagg gtctgcatca gccgcggcag gccggcctcg
    12301 tcggccggcg tggccaggag caggatgcgg tcggtggcca ggacccagct gtgcacggcc
    12361 acccggtggc gctgggcgga atcgcccagc caggcggaga gctggttgag gatgtcttgc
    12421 ggcgcaggtt ggcctggagc cgccaaaggc tggacaaaac tggcctgcac cagttggggc
    12481 agcccagggg catacagtcg gggcagacga gccataaatt cagccttgca aggggacgca
    12541 agaacggaag ggttaccgct gactatatta aatagtgccg ctgacgtcga tgtttttggc
    12601 cacggcaaat ggtgcgcatg cgtaacagtc gacctgtaac agcccgtcgc acgcccccag
    12661 cttaccgtct tcctcgaggg acgcaatcgc gggatacccc tggaaaggtg ggcggcggcg
    12721 ggaactccta cacgctggaa gggtcgaatt agcactcggg aaagcagagt gctaatatca
    12781 aatcatgctt ttgtcctacc tacccctgga ggtcgctcta tgaaacaacc cagcatgacg
    12841 tcggccgagt ctgccagcgc gctggcgttg gcagtggcca acccgggcgc gctcggcacg
    12901 atcgacgcct acatctccac gatcaaccgc ctgccggtgc tcagcgccga gcgcgagacc
    12961 gatctggccc gccgcctgcg cgaccacgac gacctcgagg ccgcccgcga actcatcctg
    13021 tcgcacctgc ggctggtggt gtcggtagcg cgccagtacc tgggctacgg cctgccgcac
    13081 gccgacctca tccaggaagg caacgtcggc ctgatgaagg ccgtcaagcg cttcgacccc
    13141 gagcgcggcg tgcgcctggt gtcgttcgcc gtgcactgga tcaaggccga gatccacgaa
    13201 tacatcgtgc gcaactggcg catggtcaag gtcgccacca ccaaggcgca gcgcaagctg
    13261 ttcttcaacc tgcgcagcat gcggcccgac ggccagacgc tcgatcccga acaggtcgag
    13321 cacatcgccc gcgagctcaa cgtgcgccgc gaggacgtca gcgaaatgga agtgcgcctg
    13381 tccggccgcg acctgtcgct ggaaggccag gacgacgacg aagacagctt cgcgcccatc
    13441 gcctacctgt ccgacgaagg ccgtgaggag cccacgcgcg tgctggcccg ccaggcgcgc
    13501 gccacgctgc agggcgaagg cctgaaccag gccctcgaag gcctggaccc gcgttcgcgc
    13561 cgcatcatcg aggcgcgctg gctgcaggac gacggcggcc tgacgctgca cgacctggcc
    13621 gccgagttca gcgtgtcggc cgagcgcatc cgccagatcg aggcggccgc gctcaagaag
    13681 atgcgcggcg cgctttcgcc cgcctgaccg catccggttc gcgcatgagg cagggcgccg
    13741 ctggccggcg ccctgcgcat ttgtaaaaac ccgctatcct tgagccttcc attttcctga
    13801 aacaggagtc tcgaggcgta tggacgttgg gctgatcgtc tttggtatcg cgggtctgct
    13861 cacgctcgtg tgcttcatgc cgcccctggc cggccgcctc aagctgccct attcggtgct
    13921 gctggccatc gtcggctgcc tgctgggcat cctcatgcac tggcatggct gggtgccgcc
    13981 ggtgctcgac gacctgatcg tcaccctcga ccgcttcgac atctcgtccg acaccttcct
    14041 gacggtgttc ctgccggtgc tgctcttcga gacctcgctg tcgatgaacg cgcgccgcct
    14101 gatggaagac atcgggccca tcctgatgat ggccatcgtg gcggtggtgg tctgcaccat
    14161 cgtggtcggc ctggccctga gcgcggcttc gtcgtacggc atcatcgtct gcctgctgct
    14221 cggggccatc gtagccacga ccgatccggt ggccgtggtg ggcatctttc gggaagtggg
    14281 ggcgcccaag cggctgacca cgctggtcga aggcgaaagc ctgctcaacg acgcggcctc
    14341 gatcgccctg tattcggtgc tggtggcggc gctggccagc ggcgcggcgg gggcgggcca
    14401 gcatctatcg gcgggcggcg tgctgcgcga cttcctctac accttcctgg gcggcgggct
    14461 ggccggctac gccatgggcc gcgtggcctg ctggctgttc gcgctgctgc gcggttttcc
    14521 cacggccgaa atcacgctca cgctcacgct ggcgtacctg tccttcttca tttccgagca
    14581 ctacctgggc gtgtcgggcg tggtggccac ggtgatcgcc ggcctggtgg tgggctcgac
    14641 gggccgcacc cgcatgtcgc ccacgacgtt cgagcacctg gccgtggcct gggagcagtt
    14701 cggcttctgg gccaattcgc tgatcttcct gttcgcctcg atgctgatcc cgcgcatgat
    14761 ggccaatgcc ggctggcggg agatcctgct ggtggtgctg gtgtttgccg ccacgctggt
    14821 cgcgcgcgcc gtggtggtgt tcggcctgct gccgctgctc aggctgatgc gcctgggcac
    14881 ccgggtcagc aattcgtaca aggccgtgat gctgtggggc ggcctgcgcg gcgccgtgtc
    14941 gctggccctg gccctggcgg tgaccgagca ggaggccatt cccgcgccgg tgcgccagtt
    15001 cgtgggcgtg gccaccacgg gcttcgtgct gatgacgctc ttcatcaacg gcaccacgct
    15061 gcgttcgctg attcgggtgc tgggcctgaa ccagctctcg tccatggagc gcgccctgcg
    15121 caaccaggcg ctcaccatcg cgctcgaaga cctgcaggag cgcaccgacg agatcgcccg
    15181 caccgaccac atcggcggcg aggtcagcgg ccgcatccac gcggtcttcg atgccagcct
    15241 ggccagcgtg cacgatgcgc agacggccca gctgaccgtc gatcagcggg tgctggtggg
    15301 cctggccacc gtggcgctgc gcgaacagga aatgttcttc gacatcctca aggcgcagat
    15361 cgtcgactgg cgcatggccg agacgctgct ggcgcgcgcc gaacggctgg aagacgcgat
    15421 ccgctcgcat ggcctgcccg ggctggaggc ggccatcgag gccgacgtgc gctatgggcg
    15481 cagtttccgg atggccttgc gcgcgtacga cctgttcggc tggcagggct ggctggcgcg
    15541 cgagctggcg cagcgcttct ccaacctgat gaccaagcgt accgtggcgc agcagctcat
    15601 caagttcacg cgcggggagc tcgcctccct gctgggcgaa gaggtggcgc agcgtatcgc
    15661 cgaggtgcac cagcggcggc tgggtctcat cgagaacgcc ttgcaggcgc tcaacctgca
    15721 gtatccgtcc tacgcccagt ggctgcagga aagctacctg ggccgggtgg cgcgcgagct
    15781 cgaacgccgc cgctaccgca cgatgctgga ccagttcctg atcagcggcg aggtctatgc
    15841 ggacctgatg aaccagttga aaagccgctg gcagcatatc gaccgccatc cgcagctcga
    15901 catggagatg ggcgcggccg acctgatcac gcgggtgccg ctgttcgagg gcctgagctc
    15961 ggagtcgctg cgggccatcg gccgcctgct gcggccgcgc ctgaccctgc cggaccaggc
    16021 gatcctggag ggcgggggct acggcgaggc catgtgcttc gtggcctcgg gcgcggtggc
    16081 ggtgcacctg ccggatggca cggcggtgga attgggcagc ggcgagttct tcggcgagct
    16141 ggccctgctg ggcgagctgc aggtcaagcc caaagtgacc tcgctgggct acagccggct
    16201 gctgatgctg tcggcgcggg atttccacgc gctgctggcc aaggatgcgg acctgcgcgg
    16261 gcgcatcgaa gtggtggcca agcagcgcat gcgggccatt gaggtgtgga agcagttttc
    16321 gcagcagccg tcgccggatg gggcggcgac gtaatgtgta ttgttgagtt cttgctcagg
    16381 acatcaatgc ttcgacgatc actagcgcgg cttcgagcgt gaattccccg gcctcgatcc
    16441 tgccgatgac gtcgtctatc ggcatggcgg cgatctccat gacctcgccg tcgcggttga
    16501 cgggctgcgc atcgggcgcc agcacgcagc ggctcaccag caggtcttcc acctgatagc
    16561 cctcgggcag ccggcggtgc aggcgcagga tggtgcgcag ggggccgcgg cctcgcaggt
    16621 gcggggcgtc caggccggct tcttcgccgc attcgcgttc cagggccagg tccaggggct
    16681 cgttgctgcc cgccagtccg ccgaccaggg tgtcccacag gccggggtcg gtggctttgc
    16741 tcagggcgcg gcgggcgacc cacaggcggc cgtcgggcgt ccaggcgttg aggtgcaccg
    16801 cataggtgag caggcccagg gggcgcatgg cggcgcgttc gatcgcgccg agatgcgcgc
    16861 cgtcggcatg gatgtccagc agttcattgc gccagccgcg caggcagcgg gcttcgcgca
    16921 gcacttgcgc cacctgcgcg agcgaggcgt ccagccgcgg gccgggcgcg aggcccggca
    16981 gcagctgcac ggcatcgcct tcggtggcgg ccaggccggc ggcgtgcagg gccgcgcggg
    17041 cggcctgggt gatccagccg cagcggcggc cggccaggac cagcggcagc gccagggcgg
    17101 gcggaggttc gctggcgcgg gcgcgcaggg cgtcgtacag cggggcgagg cgggcgagag
    17161 gaatgcggtg catgatggcg cggattttag tgcagcgccg tgtaggcctg cccgggcgtg
    17221 acgccaacgc gtcaggtgcc tatgcaaaaa tgcggggatg gaccgcatta cccatcgcta
    17281 ccgcaggctg gttttcctca cctggtttct gacgctggac ctcatcatgt tcggcgcctt
    17341 cgtgcgcctg gaggacgccg gcctgggctg ccccgactgg ccgggctgct acggcagcgc
    17401 ctcgcccgtc ggctcgctgg ccgacatcca ccaggccgcg cacgccatgc cctacggccc
    17461 ggtttccctg tccaaggcct ggatcgagat ggtgcatcgc tatgtgggcg cggccctggg
    17521 gctgctgatc atcgccatcc tctacatggc ctggcggcat cgccgccatc tgggacgttc
    17581 acccgcgctg gccacggcgg ccctggtggc cgtgtgcctg cagggcgctt tcggcgcctg
    17641 gacggtcacg caccggctca tgcccgtggt ggtgacggcg cacctggcca tcggcctgat
    17701 cgtgctggcg ctcatgacct ggctggccgc ccgcgagaag ccgcacctgg cgctggccgc
    17761 ggcggcggcg cggctgcggc cctgggcggc cgcggggctg gcgctcctgt ccgtgcagat
    17821 tctgctgggc ggctgggtca gcaccaatta cgcggccctg gcgtgcatgg actttcccac
    17881 ctgccagggc gcctggctgc ccgcgatgga tttttccggc ggcttttcct tgatccgcgc
    17941 gctgggcgaa ctgccctcgg gcgaggtgat ctcccaggag gcgctgacgg ccatccactg
    18001 gacgcaccgc aatttcgcct tcgtggtgtt cgtctacctg gccgtcctgg gctggcgcct
    18061 gcgcgccgag gcgggcctgc gcggcccggc cacgctgctg ctggccctgt tgctggcgca
    18121 ggtgtgcacc gggctcacca ccattttctt ccagtggccc ctggccgtgg ccgtgctgca
    18181 caccggcggg gcggcggcca tgaccctggc cctggtcctg attctggtgc gcctgggcac
    18241 ggccggcggt ccgccggtgc gggcggactg gacgcgggcc ggggccctgt aacatctgcc
    18301 ggctaaaata gcaggatcat gaccgccgtt accgcttctt cgcccaccgg actgctgcgc
    18361 caatacctcg tgctgaccaa gcccaaggtc actcagctgg ccgtgttctg cgcggtcatc
    18421 ggcatgttcc tggccgtgcc cggcgtgccc cgctgggacc gcgtggtcta cggcgtgctg
    18481 ggcatctggc tgctggccgc cgccgccttc gccatcaatt gcctcatcga gcaggaagtc
    18541 gatgcgcgca tgacgcgcac ggcgcggcgc gccacggccc agggcaccat ctcgtccttc
    18601 cacgtcctgg gactgtcggg cttgctgggc ggcgcgggca tgctggtgct ctactggctg
    18661 gtcaacccgc tcaccatgtg gctgaccttc gccaccttcg tgggctacgc cctcatctac
    18721 acggtcctgc tcaagccgta cacctcgcag aacatcgtca tcggcggcct ggccggcgcc
    18781 atgccgccgg ccctgggctg ggcggccgtg gccaatgccg tgccggccca ggcctgggtg
    18841 ctggtgctca tcatcttcgt ctggacgccg ccgcatttct gggccctggc cctgtaccgc
    18901 gagcaggact acgccaagtc aggactgccc atgctgccga tcacgcacgg gcgccgctat
    18961 acccagctgc agatcctgct ctacagcctg ggcctcctgg ccgtcacgct gctgccctat
    19021 gccatgcgca cgagcggcct gccgtacctg ctgtgcgcgc tggtgctggg cggcctgttc
    19081 gtgcggcatg cctggcgctt gttccagtcg tatagcgacg cgcaggcccg ccggctgttc
    19141 cgctattcca ttctgtacct gtcgctgttg ttcgccgcgc tgctggtgga ccactgggtc
    19201 gggctgctcc tggcgtgagg ctgccatgaa accgaccctc gccggccgcc gccgggtgct
    19261 gcgcgccgcg gcgtcgggcg gcctggtcct ggccttgccg ctggcgctgg ccgcctgcgg
    19321 cgacaaggga ccggtcttca agggcagcga cgttacgggc acgcacctgg gccgcgcgct
    19381 ggcgctgacc gacttcaacg gccaggcgcg cacgctggcc agcttcgccg gcaaggtgct
    19441 ggtggtgttc ttcggctaca cgcaatgccc cgacgtctgt ccgacctcgc tggccgagct
    19501 ggccgacgtc atgcgcaagc tcggtcccga tgccgaccgg gtgcaggtgc tcttcatctc
    19561 ggtcgatccc gagcgcgaca cgcccgagat actcaagcag tacaccaccg ccttcgatcc
    19621 gcgcttcctg gccttgcgcg gcacggccga cgagacccgc cagacggcga cctcgttcaa
    19681 ggcctattac gccaaggtgt cgagcaaggg cggcagcgcc gatgaatact ccatggatca
    19741 cacggcggcg ttctacctgt tcgacggcaa gggcgaggca cgggtgctcg ccagccacag
    19801 cagcagcgcc gacgacctgg cacaggacat caaggcgctg ctgtgaccgg cgcgatagcc
    19861 gggcgtggcg gtttgccccg gcggccgtca gctgacggag gtcagccagg cctgataggc
    19921 cgcgcgcgcg gcgggcgcca gttcgtcggc gccgatctcg ggccgatggc cgatgatcat
    19981 caaggcatag ggagaggcca gcgcggcggc ttccgggcac agccgggatt cctcgccttg
    20041 ggcaggcgcc cggttgcgcc agtagttgat ggcctcttcg agctgaggca gggtgatgcg
    20101 ttccatgccg ctattgtccc agaaaccggc gtaatatcgg gcggcaggcc cacaggagac
    20161 agcatgtccg attccaacaa cgccaatcct ttcgttctgc ccggcatggg gcagtcgtcc
    20221 gacctgtccg gcaacccgct cctggccagc atggaaatga tgcgccaggc ctgggccggc
    20281 ctggcgggcc cgggcggcct ggcgcagatg ctgcccatgg cctcgcccat gaataccgaa
    20341 gaactcgagc gccgcatcgc cgaactgcgc gccgtcgaaa gctggctgcg cctgaacctc
    20401 tccatgctcg acagcaccat ccagggcatg gaagtgcagc gcgccaccat cgccaccctg
    20461 cgttctttcg tcgacaaggt gtcctccatg gatggctcgt ccctggcgcc gggcgatcct
    20521 tccccgctgg aagtcgcgct ggggatcaat cccgcatcgg ccaaggcgcc gtccggggcc
    20581 gaggcctccg gcaagccggc cggcgaagaa aaggccggcg agtcgttcaa cgcggcggcc
    20641 cagtcggcct cgcaggcctg gtggaacctg ctgcagcagc agttcaacaa tattgccgcc
    20701 gccacgacgg ccaacatgcc cggcgcccag ccgggcgagc agtccgcggc ggggcagccc
    20761 aaggcggccg cccagccggc ggtccggccg acgacaaagg cccggaccac cgcctcgaaa
    20821 cccgcaccac gcaagaaacc cgcaccacgc aagaaagccg cgccgcgcaa gaaagccgca
    20881 ccctcgtccg atcaatcccg atagccaaat cgacccatca agtccctcat gttgaatgta
    20941 gtgatccttg ccgccgggct cggcaagcgc atgaagtccg atctgcccaa ggttttgcac
    21001 atcctggcgg gcagccccat gctgacccac gtgctcggca gcgcgcgcca gctcgcgccg
    21061 gcgcgcatcg tggtggtggt gggccatggc gccgaacagg tccgcgcggc ctatgaaggc
    21121 cagcccgacc tgtgctttgc cctgcagcag ccgcagcagg gcacgggcca tgccgtgcgg
    21181 caggccgtgc cgcaattgct cgagggcgat ggggctggcg acgtcacgct ggtgctctac
    21241 ggcgacgtgc cgctggtgca gcccggcacg ctcgagcgcc tcctggccgc gcgcggccag
    21301 ggcgtggccg tgctgaccga agtcctgcag gacgccgcgg gctacggccg catcgtgcgc
    21361 gacgcccacg gccaggtgct gcgcatcgtc gagcacaagg acgccaacga ggccgagctg
    21421 accatccacg aagtcaatac cggcatgctg gcggcgccca cggcccgcct gaaagactgg
    21481 ctgggccgcc tcggcaacga caatgcccag ggcgagtact acctcaccga catcatcgga
    21541 ctggccgtgg ccgacggcgt gcccgtcaag gccgcgcatc ccgacgccca gtgggaaacc
    21601 atgggcgtga acagccgcat gcagcaggtc cagctcgagc gcgcctggca gcgcgagcag
    21661 gcgcgccgcc agctcgaggc gggcgtcacg ctggccgacg ccgagcgctt cgacgtgcgc
    21721 ggcagcctgg cgtgcggccg cgacgtcttc atcgacgtgg gctgcgtgtt cgagggccgg
    21781 gtcgaactgg gcgacggcgt gcgggtcggc ccctacagcg tgctgcgcaa tgtgtcggtg
    21841 caggccggca cccgcatcga ggcctacagc cacctggacg acgcccgcat cggccgcgac
    21901 gcccacatcg gcccgtacgc gcgcctgcgt ccgggcgcct cgctgggcga ccgcgtccac
    21961 atcggcaact tcgtcgaggt caagaacagc gtggtgggca acgacagcaa ggccaaccac
    22021 ctggcctatg tgggcgacac cgacatgggc gagcgcgtca acgtcggcgc gggcaccatc
    22081 acctgcaatt acgacggcgc caacaagtgg cgcaccgtca tcgaagacga cgtattcatc
    22141 ggctccgact cccagctggt cgcgccggtg cgcgtaggcc gcggcgccac gctgggggct
    22201 ggcaccacgc tcacgcgcga tgcgccgccc gatcaactca ccatttcacg caccaggcaa
    22261 ctcagcatcg aaggctggaa gcgtcccgcc aagaaaaaat gaccgaagca gcagatacac
    22321 cgcgggcagc tccccaggac ggattgcccg cgccgcgccg ttattgggcg gcaggcacca
    22381 tcctgtgcgg catgagcctc actgtcctgg attccaccat cgtcaacgtg gcgctgccca
    22441 ccatcgccga cgacctcggc gtgcccgcct cgcgcgtggt gtgggtggtc aacgcctaca
    22501 gcctcaccat cgtgatgctg ctgctgctca tgtcgtccct ggccgaacgg ctgggcttca
    22561 agcgcatgtt cgccttcggc gtggtggtgt tcatgctgtc gtcgctgggt tgcgcgctgt
    22621 ccacgacgct gtggcagctc aacttttcgc gggtctgcca gggcgtgggg gcggcgacgc
    22681 tgatgtgcat gttcggcggc atggtgcgca atatctatcc catccgcctg ctggggcgcg
    22741 gcatcagcct caactccacg gtggtcgcgc tgatgtcggt gctggggccg tcgatcggtt
    22801 ccctgatcct ctcggtggtg tcctggcgct ggattttcgc cgtgaacgtg ccgatgagcg
    22861 tgctgctcct gttcggcctg cgcgtgttgc ccgacattcc tcgcaccaag ggcacgctcg
    22921 attgggtgag cgccctggtg tccatggcgg ccctgggcac gttcatcatc ggggtcgaca
    22981 acctggccga gaacctgccc aaggccctgg ggctgatcgt cgtggctgcg ctgctggccg
    23041 tgatcctgat gcgccgcgcg cgcgggcaga ccgcgccgct ggtgccggta gacctgctgc
    23101 gcatcaagcc cctggcctat gccgtggcgg cctcggtgtt cacgtttgcg gcgcagacca
    23161 cgtcctatgt ggccctgccg ttttatttcc tgcacacgct cgaccgcccg caactgcaac
    23221 tgggcatgct gatgggcgcc tggcccatcg gcaccgcggc gatcgcggtg atcgccggcc
    23281 gcctctccga ccgctattcg ccggcgctgc tcagcggcct gggcgcgggg gccatggcga
    23341 tcggtctgct ctggatcgcg ctgctgccgc aggacgcgtc cactgcgtcg atcatcggcg
    23401 gcatgttcct gtccggcatg ggtttcggtt ttttccagac gcccaataac cgcgccctgc
    23461 tgggttcggc gccgcgccag cgcagcggcg cggccggcgg cctgcaggcc acgacgcggg
    23521 tgttcgggca gagtttcggc acggcgctgg tggccgtggc ctacggcctg agcctcgggc
    23581 atggttcgac catcgccctg atcctggcct cgctttgcgc ggtgctggcc gtgggggtca
    23641 ataccgtgcg ttcggccaag ctggcggcca tccagccctg acggggcatc gacgagcctg
    23701 acccatgccc gccgacacgc ccgcacccgc cgcgcagccc aggctgtcgg tcgtcatcat
    23761 cgcctgcaac gagagccgcc atatcggcgc ctgcctcgac tcggtggcct tcgccgacga
    23821 gcgcatcgtg ctcgattcgg gcagcagcga cgacacctgc gacattgccc gcgcgcgcgg
    23881 cgcgatcgtg cacgcgacgc cggactggcc cggcttcggt ccgcaaaaga accgggccct
    23941 ggccctggcc acgggcgact gggtgttgtc gctcgacgcg gacgagcgcg tgccgcccga
    24001 gctggccgac gagatccgcg cggtgctgcg cgcgccgcag gcccaggcct atcgcatggc
    24061 ccgcctgtcc gaagccatgg gccgcgccat ccggcatggc ggctggtggc ccgaccatgt
    24121 gctgcggctg ttccgccgcg gcacggcgcg gttttccgac gacgccgtgc atgaaaaggt
    24181 ggtcgccgaa ggcgaggtcg gcctgctccg ggggcatctg ctgcactaca cctacgacag
    24241 catcgacgcc atgctggcca agatgaaccg ctattccacc gacagcgcgc agatgatgct
    24301 gcagcggggc aaacgcgcga cggcgctggg cgccatcggc catggcgtgt ggaccttcat
    24361 ccgcatgtat gtgctgcgcc gcggctttct cgacggctgg cagggcctgg ccatcgccag
    24421 caccggcgcg gccggcagtt tcttccgcta tctcaagctg ctggacctgc ggcgccggaa
    24481 ctgagccgct tgccgccggc gggctctccg taccttccac acctattgcc tacatgcctg
    24541 ggtaatatag ggaggcccgc gtcgcgggct tcaccgatcg gtcggccagg tagccggtct
    24601 ggcggcacgc cgccagaccg ggacgattgc ctgattcctt tcccgcgtcc ggccgtcccg
    24661 catggcgtgg ccggtgtttt tgcgaagcat catggatcat caactcttcg agtgcggcga
    24721 cttcgcgctg cacggcggcg gcatcttgcg cggcgcccgg ctcgcctaca aggcggtggg
    24781 caccctgaac gcggcacgca gcaatgccat cgtctatccc acctcgttcg gcaccaccca
    24841 cgcggacatc gaatggctgg tcgggccggg caaggcgctc gacccgcagc gctacttcat
    24901 catcatgccc agcatgctgg gcaacggcct gtcttcctcg cccagccacg gccagccggg
    24961 cacgtttccc gagctctcgc tgtgggacag cgtgcgtctg cagcaccgcc tggtagccga
    25021 gcactacggc atccagcggc tggccctggt gctggggttc tcgatgggcg ggcagcaggc
    25081 ttatcactgg gcgacgcttt tccccgacag ggtcgagcgc ctggccgtga tgtgcgcttc
    25141 ggcccgcacg tcggcgcgca accgggtctt cctgggcgag atccgagcgg cgctgacgga
    25201 cgatccagcc tggaacggac gcttcttcga ggcgcgcgcg cagcggggcg tgcgcgccat
    25261 ggcgcaggtc tatgcggact gggctctgcc ggcggagttc tatcgccgcg agacgtttcg
    25321 cgccatcggc ttccagagcc ggcaggattt cgtggcgcgg gactgggagg tttttctcgg
    25381 gcgcggcgac cccaacgacc tgatggcgat gatgggtgtc tgggagaggg ccgacgtcgg
    25441 cggcctgccg gccttcggcg gcgatacgtc tcttgccttg cggtcgatcc gcgcggcggc
    25501 gttgatcatg ccgagcacaa ccgatcaata ttttctcgcc tccggcatcg aggcggaggc
    25561 caggatgatt tccggggcgc gctattgccc catcgagacg atctggggac atcgcgtgaa
    25621 caaccccgtg cagaacccgg ccgacgcgcg cttcgtcgat gacaatctgc gtttgctgct
    25681 tgccgtgtaa ggcgggctca ggccaaggct atccgcgtct cgaacttgct gcggttgacc
    25741 gagaagaagg tgcgcacatt gcgcacattg ccctgcgccg tgaaggcgcg gtgcaccagc
    25801 gcatggtagg ccggcatgtc ctgcacctgc gcgatcacca ggaaatccgg cccggtcgac
    25861 acgcggtagc actgcagcac ggccgcctca agctgcatgg cgtcctcgaa ggcggccatc
    25921 tgctcggccg tctgcacatc cagggtgacc tcgacgatgg ccgtcagcgt ggcgcccagt
    25981 ttttcggggt cgagcagggc cacctggcgg acgatgacgc cggtctcgac caggcggcgc
    26041 acgcggcgca gacaggtcgg cggcgaggcg tgcacgcggg cggccagttc ctggttggtg
    26101 agcgcgctgt cgcgctgcat ctggtccagg atgcggcggt ccaggtcgtc caggataggc
    26161 atttctggtt gggatttttc tttaatttca ataatcctat atttatgaaa tataatttct
    26221 ctataaaata gagaagaaat tatatttcat aataaggtta atatagaagc cttatttctc
    26281 gctcttgcga gcacaattcg cacattcttc ctccccaccc cggagattta tcttatgtgc
    26341 ggcattgttg gcgccgtcgc ccagcgcgac attaccccca ttctcatcga aggcctgcgg
    26401 cgcctggagt accgcggcta tgactcctgc ggcgtggccc tgtatgtcga cggccacctg
    26461 cgccgcgcgc gcaccacgca gcgcgtggcc gaactggccg acgaagtcgc caaggaaaag
    26521 ctcgccggct acaccggcat cgcgcacacc cgctgggcca cgcacggcgg gccgtcggtc
    26581 tacaacgccc atccgcattt ctccgcccag ggcaaggacg agccgcgcat cggcctggtg
    26641 cacaacggca tcatcgaaaa ctatgaggaa ctgcgtgccg agctgatcgc cgccggcttc
    26701 gtcttcgaaa gccagaccga caccgaagtc gtcgcgcacc tcatcaacca cctctaccag
    26761 ggcgacctgt tcgaggccgt gcagcaggcc gcgcgccgcc tgaagggcgc ctacgccctg
    26821 gccgtgttct gccgcgacga accgcaccgc gtggtgggcg cgcgccacgg ctcgccgctg
    26881 gtggtgggcc tgggcaagaa cgagaacttc ctggcctccg acgcgctggc cctggccggc
    26941 accaccgacc agatcatcta cctcgaagac ggcgacgtgg tcgacctgca gctgtccaag
    27001 gtctggatcg tcgacgccgc gggcaagcgc atcgagcgca aggtccacac cgtgcagatc
    27061 catgccggcc aggccgagct cggcccctac cgccactaca tgcagaagga aatcttcgag
    27121 cagccgcgcg ccgtgggcga cacgctgcag gtggtcgagt ccatcgccac cacgctgttc
    27181 ggcgccaggg cgcatgacgt tttgcccaag atcaactcct tgctcatcct ggcctgcggc
    27241 accagctact acgccggcct caccgccaag tactggatcg aatccctcgc caagatcccc
    27301 gtcaacgtcg agatcgccag cgaataccgc taccgcgaca gcgtgcccga tccgaagacg
    27361 ctggtggtca ccatcacgca gtcgggcgaa accgccgaca ccctggccgc gctcaagcac
    27421 gcgcagtcgc tgggcatgac gcatacgctc accgtctgca acgtggccac cagcgccatg
    27481 gtgcgcgagt gcgagctcaa ctacatcacc cacgcgggcg tcgagatcgg cgtggcctcc
    27541 accaaggcct tcaccaccca gctcacggcc ctgttcctgc tggcgctgac gctcgccaag
    27601 ctgcgcggcc gcctgagccc cgaggacgaa gccctgcacc tgaagaacct gcgccacctg
    27661 cccgtggcca tcagtgcggt gctggccctc gaaccccaga tcatcgcctg ggccgaccgc
    27721 ttcgccacca aggaaaacgc gctcttcctg ggccgcggca tgcactaccc catcgccctg
    27781 gaaggcgcgc tcaagctcaa ggaaatcagc tacatccacg ccgaggccta tccggccggc
    27841 gagctcaagc acggcccgct agccctggtg accgaccaga tgccggtggt caccatcgcg
    27901 cccaacgacg cgctactgga aaagctcaag tccaatatgc aggaagtgcg cgcgcgcggc
    27961 ggcgagctgt atgtgttcgc cgacgccgac agcaagatca tcgccgagga cgacggcatg
    28021 cacgtgatcc gcatgcccga gcactacggc atgctctcgc ccattttgca tacggttccc
    28081 ttgcagctgc tggcttatca cacggcgatt gcgcggggga ccgatgtgga caagccgcgg
    28141 aacctggcca agagcgtgac ggtggagtga tcgaccctgt cgcctagaag gccgtgtcga
    28201 cggacaataa agttctatct ccgggcttgg tagctcaaaa attggcttag attggctcac
    28261 tcgacccata ctctgtacag ccgacagggc atgtgggacg tgtggcagct cttagcagtg
    28321 tgccgagcag caatcgcgaa ctattctccg ctggagagaa tgccgtacag gtcggggcgg
    28381 cggtcgcgcc
//
LOCUS       DASWPO010000030         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1613652, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000030 DASWPO010000000
VERSION     DASWPO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613652"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1294)
                     /locus_tag="VGC69_01535"
                     /pseudo
     CDS             complement(134..1294)
                     /locus_tag="VGC69_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
     gene            complement(1324..>2428)
                     /locus_tag="VGC69_01540"
     CDS             complement(1324..>2428)
                     /locus_tag="VGC69_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0293996.1"
                     /translation="LVLARIVQGMAGAMMVPVGRVILLRIVPKENLLRAMSFLSMPAL
                     LGPVIGPPIGGFMVTYMSWHWIFLINIPFGILGIAMVMHYIQEIREDDAPRLDGVGFL
                     LSGVSLATLVSAFESLGHGTMTWTQTGILLAIGLATGWLYVLHARRAAHPIIDLSLLS
                     MPTFAISVLGGNLCRFAVGAMPFLLAMLLQVGFGLSPFQAGLITFASAAGSLLMKFVA
                     APIVRRFGFRRVLTTNALLTGGFVAICALFTSTTPSWLMILILLMGGFFRSLQFTGVN
                     TLTYADVTQESMSRASSFAAMAQQLGISLGVGVAAVTLHASMVLRGGTALATPDILVG
                     FLVVGVLCMLSILSFLRLPALAGAQLNPGKMRG"
BASE COUNT          401 a          767 c          923 g          337 t
ORIGIN      
        1 acctgcatac tgcgcaatgt gataatgtga gagcgagagc agcgcctggg ctggtcccca
       61 ctcgtaaggg tcgcacaaaa ttagatggtc atgggtgcag cgaagcgcca ggatccggtc
      121 gccgccgctt gaaggaatcg agcgtggcgc gggccaggtg ggcgcgcgtc gtgccgcgcg
      181 tcatgccgac cagggtgccg cgggcatagg ggtcccagtg cggggcgccc aggccggtga
      241 aggcggggac caggaagacg tcgtcggtgt cggccacctg cgccgcgagc gcctcgatct
      301 cgtccgagcg cttgatgatg cccagcccgt cgcgcagcca ctgcaccgag gcgccggcca
      361 tgaagacgct gccttcgagc atgtaggcgg cctgcccctg ctggacgggc gagccggtcc
      421 agcccagggt ggagagcaac tggctgcgcg agatgacggg ctgcgcgccc acgttcatga
      481 gcatgaagca gccggtgccg taggtgttct tcaccatgcc gggcgtaaag caggcctggc
      541 cgaaggtggc ggcctgctgg tcgccggcca cgccggcaat ggcgatggcg ccgccgagca
      601 gatcgggcag cgtccggccg atgacgccgc tgctgggaga gatcgccggc agcacgctgc
      661 gcgggatgtt cagcagcgcc aggatgtcgt cgttccagtc gcgcgtgtgc aggtcgtaga
      721 gcatggtgcg cgaggcgttg ctgtcgtcgg tgctgtgcac cgcgccgccc gtcagatgcc
      781 acaccagcca ggcgtcgacg gtgccgaagg ccagctcgcc gcgctcggcc gcggcgcgcg
      841 cgcccggcac cttgtccagc agccaggcca gcttggtgcc cgagaaatag gcgtcgacca
      901 ccaggccggt gcgcgcctgc agccagtcgg cgtggccgtc gtggcgcagt ttttcgcaca
      961 aggcagcggt gcggcggtcc tgccagacca gggcgcgggc cacgggccgg ccggtggcgc
     1021 gttcccacag cacggtggtc tcgcgctggt tggtgatgcc gatggcggcg aggtccgagg
     1081 cctgcgcgcc ggcgttgccc agcgcgccgc gggcggtgtc gagctgcgtg ttccagattt
     1141 cgaccgggtc gtgctcgacc tggccgggct gcggatagta ctggcggaac tcgcgctggt
     1201 cgatgccgcg cacgcggccc gcgcggtcga agacgatggc gcgggagctg gtggtgccct
     1261 ggtccagcgc gaggaggaat tgcccttgtg ccatggctgc tgcctcctgg tggcgggccg
     1321 cgctcagccg cgcatcttgc ccgggttgag ctgcgcgccg gccagcgcgg gcagccgcag
     1381 gaaggagagg atcgacagca tgcacagcac cccgacgacc agaaagccca cgagtatgtc
     1441 gggggtggcc agcgcggtgc cgccgcgcag caccatgctg gcgtgcaggg tgacggcggc
     1501 cacgcccacg cccaggctga tgcccagctg ctgcgccatc gccgcgaagc tgctggcgcg
     1561 gctcatgctt tcctgcgtga cgtcggcgta ggtgagcgtg ttgacgccgg tgaactgcag
     1621 cgagcggaag aagccgccca tgagcaggat caggatcatc agccacgacg gcgtggtgga
     1681 cgtgaagagg gcgcagatgg ccacgaagcc gccggtgagc agggcattgg tggtcagcac
     1741 gcggcgaaag ccgaagcgcc gcacgatggg cgcggccacg aacttcatca gcagcgagcc
     1801 ggcggcgctg gcgaaggtga tgaggccggc ctggaaaggc gacaggccga agcccacctg
     1861 cagcagcatg gccagcagga agggcatggc gcccacggcg aagcggcaca ggttgccgcc
     1921 cagcaccgag atggcgaagg tgggcatgga gagcagcgac aggtcgatga tggggtgcgc
     1981 cgcgcggcgc gcatgcagca cgtagagcca gccggtggcc aggccgatgg ccaacaggat
     2041 gccggtttgc gtccaggtca tggtgccgtg gcccagcgat tcgaaggcgc tcaccagggt
     2101 ggccaggctg acgccgctca ggaggaagcc gacgccgtcc agcctggggg cgtcgtcctc
     2161 gcggatttcc tggatgtagt gcatcaccat ggcgatgccc aggatgccga agggaatgtt
     2221 gatgaggaag atccagtgcc acgacatgta ggtgaccatg aagccgccga tgggcggtcc
     2281 gatcaccggt ccgaggaggg cgggcatgga caggaaggac atggcgcgca gcaggttttc
     2341 cttgggcacg atgcgcagca ggatgacccg tcccaccggc accatcatgg ccccggccat
     2401 gccctgcacg atgcgcgcca gcaccagc
//
LOCUS       DASWPO010000031         8664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1256946, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000031 DASWPO010000000
VERSION     DASWPO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256946"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /gene="yihA"
                     /locus_tag="VGC69_01545"
     CDS             complement(<1..381)
                     /gene="yihA"
                     /locus_tag="VGC69_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY0293997.1"
                     /translation="MSLLHRASFFVSAARLDQLPPPGAPEVCFVGRSNAGKSSAINVL
                     CNQKRLAFSSKTPGRTRLINLFALPDPLDPDGQLGFLVDLPGYGYASVARHEKDQWAD
                     ILGGYLRDRPSLAGIVLILDIRRGV"
     gene            605..2605
                     /locus_tag="VGC69_01550"
     CDS             605..2605
                     /locus_tag="VGC69_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY0293998.1"
                     /translation="MRFAVSLLGILCIASVVGTVLQQNRPDSTYVDQFGPFWFEVFDH
                     FSLWRVYNSGWFLLIMAFLVVSTSICLARNAPRMLRDARSFREHVRADGLRAFPHRFE
                     ASYAMAAQPAADALRKVLEGQGYAVRVRRDAAGLMLAAKKGSINRLGYVCAHAALVII
                     CLGGLVDGDLSLRLQMWLGGKRPVVDNMMIADVPASGRLSPRNPSFRANVLVPEGGQT
                     TSAVVMVGDGALVQPLPFLLRLRHFVVDYYSTGMPSRFASEVEVTDPASGKTFPATIE
                     VNHPLHYKGVTVYQSSFDDGGSHVKLVAYPLAGTQAQGATVQGTVGQSLSVDHAGAAA
                     AQGAGDALRLNITGLRAINVEDMSRGVPADHGQSLGKQLAAVTGSAAGKPNQNLHNVG
                     PSIDYELVDEAGQSHAFRNYMLPITLDGASVFLMGARGDDGTYRYVRIPADAQGGLTE
                     FTRLRAALADADLRHEAARRFAASNAPSGNATAALQAAADHALDTFAHGGLQAVADVL
                     QANTPAPELEHAADVVVRLIGASMAELRDLARQRAGLPPAPQTEAEAQWSRLAVAALS
                     DLARYPAPVLYVLDDFKLVQASVFQITRNPGEYGVVLGAILLIAGIFTMFFLRDRRVW
                     AWVQPAEGGSTVLAAMTTQRRTLDFDQEFERLREAATAPGRS"
     gene            2629..3813
                     /gene="ccsB"
                     /locus_tag="VGC69_01555"
     CDS             2629..3813
                     /gene="ccsB"
                     /locus_tag="VGC69_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY0293999.1"
                     /translation="MITTSSTSSAPWPAASLVSGDRRARRGRPDLSDLVFFLLLAAGA
                     GWALAHDGSAMDYYEQAILCALVPVLTWLGWLWRPLRVLMVASGAAAGVALLLYGHDL
                     ARADHVFLLKYLLSSQSAILWMSSLFILATICYWVGFFSPTAAWLGTVCTWSAVFAGM
                     TGMLVRWRESHLMGPDLGHIPVSNLYEVFVLFSLITALFYLYYERRYATRALGGFVLL
                     VITSAIGFLLWYALARDAWQIQPLVPALQSWWMKLHVPANFIGYGSFSLSAMVACAYL
                     VKQHAPGGRLARALPAPPVLDDLMYRSIAVGFAFFTVATVLGALWAADAWGTYWQWDP
                     KETWALIVWLNYAAWLHMRLIKGLRGSMAAWWALVGLVVTAFAFLGVNMFLSGLHSYG
                     QL"
     gene            4165..5454
                     /locus_tag="VGC69_01560"
     CDS             4165..5454
                     /locus_tag="VGC69_01560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0294000.1"
                     /translation="MHLGLFILGAGHHISGWRMPGAEAGSQNLDLIKRIVQTAERGKF
                     DMVFFADAVNSAPNMHPSMIVRLEPLTLLAALAGATERIGLTATAVTTYTEPYNLARM
                     LSSIDHISGGRVAWNIVTGAFAEAGENFSKAHPPHDERYLIAAEYLEVVKGLWDGWEE
                     GALVMDPKSGVFVDLSKRHELNHKGKYYSVRGPLNITRSPQGYPVIIQAGSSEAGRDF
                     AAAAAEVIYAVQQNLEASRDFINDIHRRTKLAGRDPSQVKVMPGVVPVIGSTEREARD
                     KLAQMGEFVNPDTALQVLAERLGHDLKGYPLDGPVPDLPASDGMRGHAESLKGLAKKH
                     NFTLRELRDYTSAGMGHRLLLGTPEQVADGLIEWFDAGAADGFNIMPPWFPSAFDDFV
                     EHVVPILQRKGKFRQDYSGKTLRDHLGLDMPRNRYQR"
     gene            5542..6366
                     /locus_tag="VGC69_01565"
     CDS             5542..6366
                     /locus_tag="VGC69_01565"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294001.1"
                     /translation="MNQKNLSALVLCTPVSFFLLIFFMLPMVYVLYLSLTDPGITLAH
                     YRKIFESATFLRVMFGTFRTSLIVTVVSILVGYPLAYVMATRKDRLSLFLMIAVGLSF
                     WSGFVVRTYAWLIILGVKGPVSALFIHLGVNPPPRVLFTTFSSVLGILHILLPYMVLS
                     LYSVMKKIDPGHMRTAASLGSRPWSAFTEVYFPLSLPGVVNGSVLVFTICLGFYITPM
                     LLGSSQDMMVSQLISQQIDELLAWGTASALSMVLLGVTLVLLWVYNRFVGLDRLWG"
     gene            6371..7144
                     /locus_tag="VGC69_01570"
     CDS             6371..7144
                     /locus_tag="VGC69_01570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294002.1"
                     /translation="MIPIILAVLAAVFLAAPLFVIVPMSFSTSRSFEFPPAHYGLTYY
                     ADYFSNWDWLTPTINSFIIGGLSAAITMVLVIPTSYAYVRYRFLGRNVVNQLVMLPLM
                     VPHVVSALGFYGFLAKLGLNGTIAGMVLAHTCLSVPAAFLVACATLKGFDHNLERAAM
                     SSGAGPLRTFFWVTLPVLRPGLLVALLLSFLQSFNEAVVAIFVAGRDASTLPKKMFES
                     IRVDTDPVIAVVSTLLTALVLLGVLLAQFGRMRAAKKTA"
     gene            7164..8228
                     /locus_tag="VGC69_01575"
     CDS             7164..8228
                     /locus_tag="VGC69_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294003.1"
                     /translation="MSVPYLRLRNISKSYGGDSLAVNDVSIDIAKGEFVSFLGPSGSG
                     KTTTLMMIAGFERPTQGTIEIAGEDISRKKAYERDIGVVFQNYALFPHMTIERNVAFP
                     LKMRHRQQWEIDTKVARVLDLMGLRAHAGKYPRELSGGQQQRVALARGLVFDPDVVLF
                     DEPLGALDKNLREQMQIEIKRIHKEVGITMIFVTHDQTEAMTMSDRVAVFNNGRIEQI
                     APPLEIYNHPATRFVGGFVGDSNFLSGRVAGENPDRVAVPGFGSVQMADRSTAGADVE
                     LLVRPERIRVAPLGQGQEVNSYELAVQDLVHYGDSVLILGVVGEVPLRVRVAGGLPAY
                     VVEGARVQVGWAPQDSHRFG"
     gene            8355..>8664
                     /locus_tag="VGC69_01580"
     CDS             8355..>8664
                     /locus_tag="VGC69_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294004.1"
                     /translation="MSKGISRRTLLSAAAVAAIAAPLAGVKAASKVVYLNTWGGMWTD
                     AEQKYFIQPFTKETGIEVRTVTPISYAKLKAQVTTGSYEWDVGSFDAGDWIRANNEGL
                     V"
BASE COUNT         1338 a         2823 c         2897 g         1606 t
ORIGIN      
        1 cacgccgcgg cggatgtcca ggatcagcac gatgccggcc agcgaggggc ggtcgcgcag
       61 gtagccgccc aggatgtcgg cccactggtc tttctcgtgg cgcgccaccg aggcgtagcc
      121 gtagcccggc aggtcgacca ggaagcccag ctgcccatcg gggtcgagcg ggtcgggcag
      181 ggcgaacagg ttgatcaggc gcgtgcggcc gggcgtcttg ctggagaagg ccaggcgctt
      241 ttggttgcac agcacattga tggccgagga cttgccggcg ttggatcggc ccacgaagca
      301 gacttccggc gcgccgggag gcggcagttg gtccagccgg gccgccgaca cgaaaaacga
      361 ggcgcgatga agaagggaca cgttgggggc gataaacgag gtttgcaacc cctgattgta
      421 cgagccgggg cggtttcccg catggcggct gccgatcgcc ctgccgcggc cgattgcgcg
      481 gccggcttgc gccggcagct tgttgtcatc tcggcgcggt atgcttttgc ccgatatgaa
      541 ccagattgcc gtccgctccg tcttccaccg ccttgccggc gcgctgttcg aactcatgag
      601 ttcgatgcgt ttcgccgtga gcctgctggg cattctgtgc atcgccagcg tggtgggcac
      661 ggtattgcag caaaaccgtc ccgacagcac ctatgtcgac cagttcgggc cgttctggtt
      721 cgaggtgttc gaccattttt ccctgtggcg cgtctataac agcggctggt ttctgctgat
      781 catggcgttc ctggtggtgt ccacctcgat ctgcctggcg cgcaatgcgc cgcgcatgct
      841 gcgcgatgcg cgctcgtttc gcgagcacgt gcgcgccgac ggcctgcgtg ccttcccgca
      901 tcgcttcgag gcgagctacg ccatggcggc ccaacccgcc gccgacgcct tgcgcaaggt
      961 gctggaaggg cagggctatg ccgtgcgcgt gcgccgcgac gccgcgggcc tgatgctggc
     1021 ggccaagaag ggcagcatca atcggctggg ctatgtgtgc gcgcacgcgg cgctggtcat
     1081 catctgcctt ggcggcctgg tcgacggcga cctgtcgctg cgcctgcaga tgtggctggg
     1141 cggcaagcgg cccgtggtcg acaacatgat gatcgccgac gtgccggcca gcggccggct
     1201 gtcgccgcgc aatccgagct ttcgcgccaa cgtgctggtg cccgagggcg ggcagacgac
     1261 ctcggcggtg gtgatggtgg gcgacggcgc gctggtgcag cccctgccct tcctgctgcg
     1321 gctcaggcat ttcgtggtgg actactactc caccggcatg cccagccgct tcgccagcga
     1381 ggtcgaggtc accgatcccg cgagcggcaa gacctttccg gccaccatcg aggtcaacca
     1441 tccgctgcac tacaagggcg tcacggtgta ccagtcgagc ttcgacgacg gcggcagcca
     1501 cgtgaagctg gtggcctacc ccctggcggg cacgcaggcg cagggcgcga cggtgcaagg
     1561 cacggtgggg cagtcgcttt cggtcgacca tgccggcgcg gccgcggcgc agggcgcagg
     1621 cgacgccttg cgcctgaaca tcaccgggct gcgcgccatc aacgtcgagg acatgtcgcg
     1681 cggcgtgccg gccgatcacg gacagtcgct gggcaagcag ctggccgcgg tcaccggcag
     1741 cgccgccggc aagcccaacc agaacctgca caatgtcggc cccagcatcg actacgaact
     1801 ggtcgacgag gcggggcagt cgcatgcgtt tcgcaattac atgctgccca tcacgctcga
     1861 cggcgcgtcg gtcttcctga tgggcgcgcg cggggacgac ggcacctatc gctatgtgcg
     1921 catcccggcc gatgctcagg gcgggctgac ggaattcacg cgcctgcgcg cggccctggc
     1981 cgatgccgac ctgcggcatg aggccgcgcg ccgcttcgcc gccagcaatg cgccgtccgg
     2041 caacgccacg gcggccttgc aggccgccgc cgaccatgcg ctcgacacct tcgcgcacgg
     2101 cggcctgcag gccgtggccg acgtgctgca ggccaacacg ccggcccccg agctggagca
     2161 cgccgccgac gtggtggtgc gcctgatcgg cgccagcatg gccgagctgc gcgacctggc
     2221 gcggcagcgc gccggcctgc cccccgcgcc gcagaccgag gccgaggcgc agtggtcgcg
     2281 gctggccgtg gcggccctgt cggacctggc gcgctacccg gcgccggtgc tctatgtgct
     2341 ggatgatttc aagctggtgc aggccagcgt tttccagatc acgcgcaacc cgggcgaata
     2401 cggcgtggtg ctgggcgcca tcctgctgat cgccggcata ttcaccatgt tcttcctgcg
     2461 cgaccggcgc gtctgggcct gggtgcagcc cgccgagggc ggcagcacgg tgctggccgc
     2521 gatgacaacg caacggcgta cactggactt cgaccaggag ttcgagcgcc tgcgcgaagc
     2581 cgccacggcg ccgggccggt cctgaccacg catcgatatc aagaagccat gatcactacc
     2641 tcctcgactt cgtccgcccc ctggccggcc gcctcattgg tcagcggcga ccgccgcgcg
     2701 cggcgcggcc ggcccgatct ttccgatctc gtgttcttcc tgctgctggc cgccggcgcg
     2761 ggctgggcgc tggcgcacga cggcagcgcc atggactact acgagcaggc catcctgtgc
     2821 gcgctggtgc cggtgctgac ctggctgggc tggctgtggc gcccgctgcg cgtgctcatg
     2881 gtcgccagcg gcgccgccgc cggcgtggcg ctgctgctct acggacacga cctggcgcgg
     2941 gccgatcatg tgttcctgct caagtacctg ctgtcttcgc aatcggccat cttgtggatg
     3001 agttcgctct tcatcctggc cacgatctgc tactgggtgg gtttcttcag cccgaccgcg
     3061 gcctggctgg gcacggtctg cacctggagc gccgtgttcg cgggcatgac cggcatgctg
     3121 gtgcgctggc gcgaaagcca cctcatgggg cccgacctgg gccacatccc ggtgagcaac
     3181 ctgtacgaag tgttcgtgct gttctcgctc atcaccgcgc tcttctacct gtactacgag
     3241 cggcgctacg ccacgcgcgc gctgggcggc ttcgtgctgc tggtgatcac ctcggccatc
     3301 ggctttctgc tctggtatgc gctggcgcgc gatgcctggc agatacagcc gctggtgccg
     3361 gccctgcaaa gctggtggat gaagctgcac gtgccggcca acttcatcgg ctacggctcg
     3421 ttctcgctgt cggccatggt ggcctgcgcc tacctggtca agcagcacgc gcccggcggg
     3481 cggctggccc gcgccctgcc ggcgccgccg gtgctcgacg acctcatgta ccgttccatc
     3541 gcggtgggct ttgccttctt caccgtggcc acggtgctgg gggcgctgtg ggcggccgac
     3601 gcctggggca cgtactggca gtgggacccg aaggaaacct gggccctgat cgtctggctg
     3661 aactacgccg cctggctgca catgcggctc atcaagggcc tgcgtggcag catggccgcc
     3721 tggtgggcgc tggtgggcct ggtggtgacg gccttcgcct tcctgggcgt gaacatgttc
     3781 ctgtcagggc tgcattcgta cgggcagctg taatgggcgt tcccgagccg gcataaagcg
     3841 ccggcacggt gagggcatgg gggcaggctg ccccgcaggc cctcatcctc tctcatcatc
     3901 ccgcatcgtt ggctcatgat ccgctgagcc gtggccgttc ggcctggccg gtctcgtcgg
     3961 ccggacctgt ctcgccctga aaatcccggc atgggccgcg gggttcggca tccgcgtttt
     4021 ccctaatcga gtgaaaaccc tagtccttaa aagggaggaa ttccttgtcc caaaattgaa
     4081 ctttttatac agtatgcaaa attgcccgga tgtccagggc aagcatgact caaggagcgc
     4141 gccgtggcgc cgacaaatca acatatgcat ctgggcttgt tcattcttgg cgcgggccac
     4201 catatctcgg gctggcgcat gccgggcgcc gaggccggtt cgcagaacct cgacctgatc
     4261 aagcgcatcg tccagacggc cgagcgcggc aagttcgaca tggtgttctt cgcggacgcg
     4321 gtgaactccg cgcccaatat gcacccgtcg atgatcgtcc gcctggagcc gctgaccctg
     4381 ctggccgcgc tggcgggagc gaccgagcgc atcgggctga ccgccaccgc cgtgacgacc
     4441 tacaccgagc cctacaacct ggcgcgcatg ctgtcgtcca tcgaccatat cagcggcggc
     4501 cgcgtcgcct ggaacatcgt gacaggcgct ttcgccgagg ccggcgagaa cttctccaag
     4561 gcgcacccgc cgcacgacga gcgctacctc atcgccgccg aatacctgga agtggtcaag
     4621 ggcctgtggg acggctggga agaaggcgcg ctggtcatgg acccgaaaag cggcgtgttc
     4681 gtcgacctgt ccaagcgcca tgaactgaac cacaagggca agtactactc ggtccgcggc
     4741 ccgctcaaca tcacgcgttc gccgcagggc taccccgtca tcatccaggc gggctcctcg
     4801 gaggccgggc gtgatttcgc cgcggctgcg gccgaggtga tctatgccgt gcagcagaat
     4861 ctcgaggcct cgcgcgactt catcaacgac atccaccgcc gcaccaagct ggcgggccgc
     4921 gacccttcgc aggtcaaggt catgccgggc gtcgtgccgg tgatcggctc gaccgaacgg
     4981 gaagcgcgcg acaagctcgc gcagatgggc gagttcgtca atcccgatac ggccttgcag
     5041 gtgctggccg agcgcctggg gcacgacctg aagggctatc cgctggacgg ccccgtgccg
     5101 gacctgccgg cttcggacgg catgcgcggg catgccgaat ccctcaaggg cctggccaag
     5161 aagcacaact tcacgctgcg cgagctgcgc gactacacgt ccgccggcat ggggcaccgg
     5221 ctgctgctgg gcacgcccga gcaggtggcc gacggcttga tcgaatggtt cgacgcgggc
     5281 gcggccgatg ggttcaacat catgccgccg tggttcccct cggccttcga cgacttcgtc
     5341 gagcatgtgg tgccgatact gcagcgcaag ggcaagttcc gccaggacta cagcggcaag
     5401 accctgcgcg accatctggg cctggatatg cccaggaacc gttatcaacg ctaggcagcg
     5461 ctgacgcgcc gggcaggctg cccgcctttc gaggcgggcg ccgtgctgcg tcgggcaatc
     5521 gatattcctc aaggggtggg aatgaaccaa aagaatcttt cggcattggt gctttgtacg
     5581 ccggtaagtt tcttcctgct gattttcttc atgctgccca tggtctacgt gctctacctg
     5641 agcctgacgg acccgggcat cacgctggcg cactatcgca agatcttcga gtccgcgaca
     5701 tttctgcggg tcatgttcgg cacgttccgg acttcgctca tcgtcacggt cgtgagcatt
     5761 ctggtgggtt atcccctggc ctacgtcatg gccacgcgca aagaccgcct gagcctgttc
     5821 ctgatgatcg ccgtgggcct gagcttctgg tccggcttcg tggtccgcac ctacgcctgg
     5881 ctgatcatcc tgggcgtgaa aggccccgtg agcgcgctgt tcatccacct gggcgtcaat
     5941 ccgccgccgc gggtcctgtt cacgaccttc agttcggtgc tcggcatcct gcacatcctg
     6001 ctgccctata tggtgctctc gctctacagc gtcatgaaga agatcgaccc gggccatatg
     6061 cgcacggccg ccagcctggg ctcgcgcccc tggtcggcct tcacggaggt ctatttcccg
     6121 ttgagcctgc ccggcgtggt caatggttcg gtgctggtgt tcacgatctg cctggggttc
     6181 tacatcacgc ccatgctgct cggatcgagc caggacatga tggtttccca gctgatcagc
     6241 cagcagatcg acgagctgct cgcctggggc acggcgtccg cgctgtcgat ggtgctgctg
     6301 ggcgttacgc tggtgctgct ctgggtctac aaccgcttcg tcggcctcga ccggctctgg
     6361 ggatgaaacc atgattccga tcatcctggc ggtgcttgcg gcggtcttcc tggccgcgcc
     6421 gcttttcgtc atcgtgccga tgtcgttcag cacgtcgcgg tcgttcgagt ttccgccggc
     6481 gcactatggc ctcacgtact acgccgatta tttcagcaac tgggactggc tcacgcccac
     6541 gatcaacagc ttcatcatcg gcgggctgtc cgcggcgatc acgatggtgc tggtcattcc
     6601 gacttcgtat gcctacgtgc gctaccgctt cctcggccgc aatgtggtga accagctggt
     6661 gatgctgccg ctgatggtgc cgcacgtggt atcggcgctg ggtttttacg gctttctggc
     6721 caagctgggc ctgaacggca cgatcgcggg gatggtcctg gcgcacacct gcctgtccgt
     6781 gccggcggct tttctggtgg cctgcgccac gctcaagggc ttcgaccaca acctcgagcg
     6841 cgccgccatg agttcggggg cggggccgct gcgcaccttt ttctgggtca cgctgccggt
     6901 gctgcgcccg ggcctgctgg tggccttgct gctttcgttc ctgcaatcct tcaacgaggc
     6961 ggtggtggcg atcttcgtcg ccggccggga tgcctcgacg ctgcccaaga agatgttcga
     7021 aagcatacgc gtcgacacgg atccggtgat cgccgtcgtc tcgaccttgt tgaccgcact
     7081 ggtgcttctg ggggtgctcc ttgcgcagtt cgggcggatg cgggccgcga aaaagaccgc
     7141 ttgaaatgga aagtcgagaa aacatgagcg ttccttatct tcgtttgcgc aatatcagca
     7201 aatcgtacgg cggcgattcc ctcgcggtga acgatgtcag catcgacatc gccaagggcg
     7261 agttcgtcag cttcctgggc cccagcggtt cgggcaagac gaccacgctg atgatgatcg
     7321 ccggcttcga gcggcccacc cagggcacca tcgaaatcgc cggggaagac atcagccgca
     7381 agaaagccta cgagcgcgac atcggcgtgg tgttccagaa ctatgccttg ttcccgcaca
     7441 tgacgatcga gcgcaatgtc gcgttcccgc tcaagatgcg gcatcgccag caatgggaaa
     7501 tcgacaccaa ggtggcccgc gtgctggacc tgatgggctt gcgcgcgcat gcgggcaagt
     7561 acccgcgtga actctccggc ggccagcagc agcgcgtggc cctggcgcgc ggcctggtgt
     7621 tcgacccgga cgtggtgctt ttcgacgagc cgctgggcgc gctcgacaag aatctgcgcg
     7681 agcagatgca gatcgaaatc aagcgcatcc ataaagaagt gggcatcacc atgatcttcg
     7741 tgacccacga ccagaccgag gccatgacca tgtccgaccg ggtggcggtc ttcaataacg
     7801 ggcgcatcga gcagatcgcc ccgccgctgg agatctacaa ccaccccgcc acgcgcttcg
     7861 tcggcggctt tgtcggcgac agcaactttt tgtccgggcg cgtggcgggc gagaatccgg
     7921 atcgggtcgc cgtgccgggc tttggctcgg tgcaaatggc ggatcggagc acggccggcg
     7981 ccgacgtcga gctgctggtg cgtcccgaac gcatacgcgt tgcccctttg gggcagggac
     8041 aggaggtcaa cagttatgag ctggccgtgc aggatctggt gcattacggc gatagcgtgc
     8101 tgatcctggg cgtggtgggc gaggtgccgt tgcgggtgcg tgtggcgggc ggcttgcctg
     8161 cctatgtggt ggaaggcgcg cgggtgcagg tgggctgggc gcctcaggac agtcaccgtt
     8221 tcgggtgacg gcgcgagtag tggcagtgca taggcgggga gcgggcccga atgggatacc
     8281 actaggatgg gacaccaccg gggagcgtct tccctatttt cggtttcatg tcttgccttg
     8341 ataaggatgc aatcatgagt aaaggaatct cgagaaggac cctgttgtcc gccgctgccg
     8401 tcgcggcgat tgccgcgccg ctggcgggtg tcaaggccgc gtccaaggtc gtgtacctga
     8461 acacctgggg cggcatgtgg acggatgccg agcagaagta tttcatccag cccttcacca
     8521 aggaaaccgg catcgaagta cgcaccgtga cgccgatttc atacgccaag ctcaaggcgc
     8581 aggtcacgac gggcagctat gaatgggacg tgggctcgtt cgacgccggg gactggatcc
     8641 gggccaacaa cgaaggcctg gtcg
//
LOCUS       DASWPO010000032        15423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_95949, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000032 DASWPO010000000
VERSION     DASWPO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15423
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95949"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VGC69_01585"
     CDS             <1..1051
                     /locus_tag="VGC69_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEY0294005.1"
                     /translation="KMAMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAV
                     LMAKQSLMDDIDMYELLERGPQNKTEALRIELYEKVNALGIGAQGLGGLTTVLDVKIN
                     MFPTHAASKPVAMIPNCAATRHAHFVLDGSGPAHLQAPALSEWPDVHWAPDYNKSKKV
                     DLDTLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYV
                     GPVDPVRDEVVGPAGPTTATRMDKFTDMMLEKTGLISMIGKAERGPVAIESIKNHKSA
                     YLMAVGGAAYLVSKAIRSAKVVGFEDLGMEAIYEFDVQDMPVTVAVDSSGTSVHNTGP
                     KAWQARIGKIPVAVA"
     gene            complement(1125..2900)
                     /locus_tag="VGC69_01590"
     CDS             complement(1125..2900)
                     /locus_tag="VGC69_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0294006.1"
                     /translation="MTPLIEALRDSAGPAVEDFYGKLSLLPKSRVILGMLDPGELQHL
                     KSRQVQNLLSMADPSLQDEDHAATALRVGRIHAIVGLDKEELAQSRSILSAAVYQRIA
                     QNVSNQDLSLLNQRLQNDLTWQLKAYQAIEDAQKTALLELMRVIWESANYTDLIHQVV
                     HILSQHDEVAGCAIGRPDEHNVFHFEVASGNIQDYLDDRQGAPETQIMVNADDPRGQG
                     PVGRAWRSGHIERMINFKTQQHASVWREQSLKLGLRSCAAVPLYAPGRATVAILLLHS
                     ALPGGFIGPHQASFLDMLQTLLGCALERLGAKDGMRETIPIKNRQHLAALVRSDSLIN
                     YYQPLLDTHTGKVRKVEALARLIDGRRLLLPEHFLPALVLDDLLEIYVRGLEYALRDK
                     AGWTGSGIELDLSINLPPAGLSDIRYYEATEAALARHHCPPGQLTLEILENETVSLSH
                     GQPAILTRFKDLGVKLAQDDLGSGHSGLTRLRQLPFDWIKLDRDMVHIKDDDNALEVL
                     RFLHQLVRLGHAMGKQVVAEGIETAELAEALGMLGVDLIQGYAVAKPMAAGRIAEWAR
                     AYSFQPGKSGHAALPALARQLVEET"
     gene            3041..4213
                     /gene="bcsZ"
                     /locus_tag="VGC69_01595"
     CDS             3041..4213
                     /gene="bcsZ"
                     /locus_tag="VGC69_01595"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HEY0294007.1"
                     /translation="MTILRGIGSINGLAGALLVALAVGARAAPVTAQACDWPDWNIFK
                     STAMSADGRVTDASENGITTSEGQSYGLFFALVANDRASFDKLLAWTENKLAGGDLAA
                     RLPAWLWGRRSAGQPADWDVLDTNSASDADLWIAYTLLEAGRLWHERRYRALGSIMAR
                     AIVRQETAQLPGLGRTLLPGADGFHPAPDLWRLNPSYVPLQLMRGLASALPHQAEWKA
                     LVDTSAHLVNDTAPRGFSPDWVLYRSGHGFEPDVQTRAESAYNAVRVYLWAGMLAPDE
                     PLRAATLRTFAPLADYVSANGFPPERIDTRTGESGPNAGNAGFSAAVAPYLAALGRDD
                     LARAQQERARAAAANAPLGYYSSVLTLFGIGYLEGRYRFDAGGRLVPAWTATCPGR"
     gene            4329..5804
                     /locus_tag="VGC69_01600"
     CDS             4329..5804
                     /locus_tag="VGC69_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY0294008.1"
                     /translation="MGVVLACDLGGTTLRVALVDAQGAVLADAMVDSPAGSSGEGWAE
                     IDAADWWRAYAEAVEALAQNAGALFDAAEALATCGVTRTQVFLDAAGRALRPAITWRD
                     ARAADVMDEVLAAVPPGHAERAALNPFHPLARLAWLRRHEPAAHARLRSVAEPKDYLN
                     LRLTGVLATDEISSARLAAVRGAPLAAVGCADAVLPAVLPPEGEVGRVAAGLPGALGR
                     LAGRPVYACAHDTWAAVVGLGALRPGHAYNISGTTEVFGVIGTQPAQAPGLMTVDWGA
                     GLHQIGGPGQNGADTVAWLLQLLARQAQGKTDVAGTLDALLARPRDPQPLLFLPYLQG
                     ERVPYWDAGLRGAFVGLNRRHGAGDLAWAVLEGVAFLNRIVLERGEQAAGSPVSEIHF
                     GGGAAANPVWCQVKADVCERPLVVGAQAQPGIVGAAAVAWTGLGRYASLDAAQQALAR
                     PSRRHEPDAARSAAYRRLFELYRRAEDALAPISRELAAHAA"
     gene            5794..6738
                     /locus_tag="VGC69_01605"
     CDS             5794..6738
                     /locus_tag="VGC69_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294009.1"
                     /translation="MLRNVLERYGTALAGLVLVLFFAWTAPNFASAGNLLNILKETSF
                     LAILSLGFTLALINAELDLSVADVASLAAVVCGALIHGGQPVPLAVAGGLGAGLGCGI
                     INGLVVTRLRVPSLIATLGMAAMARGFGFMLTEGVSFVGRWPQSFTGLGRGVLLGIPA
                     LVWWLAAVALGSLFLIKCTRTGARMTATGEASEAARLAGINTLRMKGLGLALAGLLAG
                     LTAVLLAASLSSAAPNMAGDYFLYAIAAVLLGMTMFEPGRANVPGTLAAALTLKVLGN
                     GLVLLGAPYYVQDIVLGFIIIVSVAVSAAVLKKAAFKF"
     gene            6755..7711
                     /locus_tag="VGC69_01610"
     CDS             6755..7711
                     /locus_tag="VGC69_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0294010.1"
                     /translation="MRKRSKHLIAALAAAPLLLAGAAAHAFELGIVAFQMSSETHARV
                     ANAAAAEARAKGWNVTLLNSEGALPKHAEQLETLINKKVDAIIVAMGKPVEAEAQLKE
                     AQDHGIPVIGVMSGASPYMLFDVETNEYKVGAESALYLLGQLGYQGGVLESRFDGNSG
                     TRIRGKVLDAVLSENQAVKDVGNFSMARTQSWQDDVRNGMRALIMQNTGKFKGIWVSF
                     DGQAYVIDDLLQQQGMKKGDVALVSTDGGAETYRRIADPQSLITATVMIPFETMGKTA
                     VDSLDKIVVQKQPRESVTAGPYLFADAVLVDKNNVQQFLKKE"
     gene            7708..8475
                     /locus_tag="VGC69_01615"
     CDS             7708..8475
                     /locus_tag="VGC69_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294011.1"
                     /translation="MTGRTPILSLRQVRKSYGAVEALRGVDLDVHRGEVLAICGDNGA
                     GKSSLIRVISGAQAASGGEIRLKGEVVRLRSPQDALARGIATIYQDLALAPRLSIAEN
                     VFMGSELVRRVLPGVRVLDRRAMAEQARGYLQRLSLAVDDMRRPVERMSGGQRQAVAI
                     ARALRWDAEVIIMDEPTAALGVKETALVLGLVRTLREEGRTVILISHNMADVVALADR
                     VAILKGGVKVAEREVAGLDADRLAHMVMTGRDAAVAA"
     gene            complement(8500..8751)
                     /locus_tag="VGC69_01620"
     CDS             complement(8500..8751)
                     /locus_tag="VGC69_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0294012.1"
                     /translation="MRKVDAHIIAEHEYDELPELTDEMMTRAVVNKGGRPRSASPKVL
                     LSVRYSQEVVDFFRATGDGWQSRMDGVLRNYVKRHRKPA"
     gene            complement(8753..8974)
                     /locus_tag="VGC69_01625"
     CDS             complement(8753..8974)
                     /locus_tag="VGC69_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0294013.1"
                     /translation="MDFEDAVLAFEGATLQIEDTRRDYGEKRILCYGMLAGRMIVVGY
                     VARGDSLHIFSMRKTNDREKNRLGPLLAI"
     gene            complement(9031..9498)
                     /locus_tag="VGC69_01630"
     CDS             complement(9031..9498)
                     /locus_tag="VGC69_01630"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY0294014.1"
                     /translation="MRAYIGLGSNLGDSGTTLRTALADIAALPGIQACAASPFYRSAP
                     VDASGPDFINAVAALDTTLAPLDLLDALQAIELAHGRQRPYRNAPRTLDLDLLLYGNV
                     AMATERLTLPHPRMHERAFVLRPLHDLAPHLALPSGDISDLLKQTADQQITRL"
     gene            complement(9503..10897)
                     /gene="pcnB"
                     /locus_tag="VGC69_01635"
     CDS             complement(9503..10897)
                     /gene="pcnB"
                     /locus_tag="VGC69_01635"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY0294015.1"
                     /translation="MITTTIKKFVGRLFTPTASGPQRIGRDQHGIDRRNVSRHAVKVC
                     EVLQQHGYAAYVVGGAVRDLILGLEPKDFDVATNATPEEIRALFRRARIIGRRFQLVH
                     VVFGQEIIETSTFRAPANGEQKTDEHGRILSDNVFGTQEQDAARRDFTINALYYDPHT
                     EEVIDYHNGVQDLKKRQIRMIGDPVQRYREDPVRMLRAVRFAAKLNGTIDTATREPIR
                     TMSDLIENVPASRLFDEMLKLLTCGHAIDCLKQLRDEGLHHGMLPLLDIVLEQPGGRK
                     FIEEALDRTDARVRSGRGISPSFLFAALLWPQVESRWKQLVADGESAIPALGQAADSV
                     LDEQTEKLAIQRRFSSDMREIWFMQPRFERRQGKTIYRMIEQPRFRAACDFLQLRAAA
                     GEFDSVLAQWWMDLANANDETRAGMIEDTANLPFEAGDAPPARKRRRRPRRKPAGAGA
                     AGAAGGEPSLPDEE"
     gene            complement(10977..11678)
                     /locus_tag="VGC69_01640"
     CDS             complement(10977..11678)
                     /locus_tag="VGC69_01640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0294016.1"
                     /translation="MTAARRLALFDLDHTLLPLDSDHQWAEFLARTGRAGDPGEALRR
                     NNDLMERYNRGDLTAEQAAEFMLGLLSAHSPYELALWHEEYMREVVRPAIQSVALDLV
                     RQHLEAGDLCAIVTATNTFVTAPIARAFGVPHLIGTEPEYVAGRYTGRIAGTPSFKEG
                     KVVRVQQWLSGIGLAMADFDISFFYSDSINDVPLLERVTRPIATNPSPALRTLAERQG
                     WQVLDLFDHAQDAKS"
     gene            complement(11675..12376)
                     /gene="hda"
                     /locus_tag="VGC69_01645"
     CDS             complement(11675..12376)
                     /gene="hda"
                     /locus_tag="VGC69_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEY0294017.1"
                     /translation="MNRQLLLDVLPEPAPTLGNYIAGANGEALTAARGLTAGQALYLW
                     GPAGCGRSHLLRGLAARSGGVYLDAAQDARAFLALAQADAAADLPALVAVDDVHRMDE
                     ARQAALFALYNRWRESAAAGRAFALAVAGDRAPLAMPLREDLRTRLGWGLVFRLDPLS
                     DADKLAALSSQAAERGLQLSAEVLNWMLTHHERDIRKLTALLDALDRYSLATRRAITI
                     PLLRAMLADPESNTP"
     gene            12540..13589
                     /gene="purM"
                     /locus_tag="VGC69_01650"
     CDS             12540..13589
                     /gene="purM"
                     /locus_tag="VGC69_01650"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY0294018.1"
                     /translation="MTNPTSAPLTYRDAGVDIDAGDALVDRIKPLAARTMRPGVLAGI
                     GGFGALFEVPKKYKEPVLVSGTDGVGTKLRLAFEWNRHDTVGIDLVAMSVNDILVQGA
                     EPLFFLDYFACGKLAVDTAASVVGGIARGCELAGCALIGGETAEMPGMYPEGEYDLAG
                     FAVGAVDKPAIIDGKSIRPGDVVLGLASSGAHSNGYSLVRKILERAGARPEQDFHGQK
                     LVDVVMAPTRIYVKQVLAALEKHGPAIKGLAHITGGGLLDNVPRILQPGLSARLHRDA
                     WELPLLFQWLQQQGGVADGEMHRVFNCGIGMVIVVPPVAADAIAATLREQGETVYALG
                     EIVPQADGMAQTVVV"
     gene            complement(13592..14350)
                     /locus_tag="VGC69_01655"
     CDS             complement(13592..14350)
                     /locus_tag="VGC69_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294019.1"
                     /translation="MSGKLLEGRVAVVTGASRERGIGLATARLFAEHGARVALLDLDE
                     GEAAQAARSVGPEHLGVGCDVSDPAACRAAVARVLDWAKRIDALVNNAGVTQRAGILD
                     ITGADYERVTGIILKGALQMSQSVIPPMIEQGGGALIHISSMSAQQGGGVFGGGHYCA
                     AKAGVLGLSRAIAKEFGPKGVRSNAVTPGLILTDFSRGANSDQNKHEWAREFPLARVG
                     QPADIAGACLYLASDLSAFMTGATLDVNGGAYMR"
     gene            complement(14418..>15423)
                     /locus_tag="VGC69_01660"
     CDS             complement(14418..>15423)
                     /locus_tag="VGC69_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0294020.1"
                     /translation="AGVSLGTASRALNRTGPVSTQAIAAVSRVARELGYVPNALAQSM
                     RTRSTGVVGLLVSEISNPLYAGIITAVEARLFSAGYTLLLASTHGDANREKSLIDLFR
                     RRRVDGLILGPCDSEKPEMLERLASDVPVVALDRDITAEGTGVHVDHYHGALQATRYL
                     LTLGHRRIALLTPGSALRPGRERIAGFEDAFAAQGVQPDPRLIRAESSSMEFSFSEAL
                     ALLSSANEPTAFLCLGTRILSGVLQAIRHSGRTVPDDVSVISIGDTDLSQLFTPAITS
                     LTWDLAALGTACAEVLLRNLPAREEKEPERILITTQMILRESCMPPPLAVRTRTDARV
                     "
BASE COUNT         2313 a         4976 c         5574 g         2560 t
ORIGIN      
        1 caagatggcc atgctcaatc ccagcgactc gatcgtggac tgggtgctca agaccgtgcc
       61 gaccatgggc gcgggctggt gtcccccggg catgctgggc atcggcatcg gcggcaccgc
      121 cgagaaggcc gtgctgatgg ccaagcagtc gctgatggac gacatcgaca tgtacgaact
      181 gctggaacgc ggcccgcaga acaagaccga agcactgcgc atcgagctgt acgaaaaggt
      241 caacgcgctg ggcatcggcg cccagggcct gggcggcctg accaccgtgc tggacgtgaa
      301 gatcaacatg ttccccacgc acgcggcctc caagcccgtg gccatgatcc ccaactgcgc
      361 ggccacccgc cacgcccact tcgtgctcga cggctcgggt ccggcgcacc tgcaggcgcc
      421 cgcgctgtcc gagtggcccg acgtgcactg ggcgccggac tacaacaagt ccaagaaggt
      481 cgacctcgac acgctcacca aggaagaagt cgccagctgg aagccgggcc agaccctgct
      541 gctcaacggc aagatgctca ccggccgcga cgccgcgcac aagcgcatcc aggacatgct
      601 ggccaagggc gagaagctgc ccgtggattt caccaaccgc gtcatctact acgtgggccc
      661 ggtcgacccg gtgcgcgacg aagtggtggg cccggccggc ccgaccacgg ccacccgcat
      721 ggacaagttc accgacatga tgctcgagaa gaccggcctc atctccatga tcggcaaggc
      781 cgagcggggt ccggtggcca tcgagtcgat caagaaccac aagtcggcct acctgatggc
      841 cgtgggcggc gccgcctacc tggtgtccaa ggccatccgt tcggccaagg tggtgggctt
      901 cgaagacctg ggcatggagg cgatctacga attcgatgtg caggacatgc ccgtgaccgt
      961 ggcggtcgat tccagcggca cgtcggtgca caacaccggc ccgaaggcgt ggcaggccag
     1021 gatcggcaag atcccggtcg cggtggcctg agccgccagg gaccgaacgg ccgcaaggcc
     1081 atgaaaaggc gggccgcctg cgtgggcggc ccgcctttta cctcctacgt ttcttcgacc
     1141 aactgcctgg ccagtgccgg cagggcggca tggccggact tgcccggctg gaaggagtag
     1201 gcccgcgccc attcggcgat cctgccggcg gccatgggtt tggccacggc atacccctgt
     1261 atcagatcga cgcccagcat gcccagcgcc tcggccagtt ccgcggtctc gataccctcg
     1321 gccacgacct gcttgcccat cgcgtggccc aggcggacca gctggtgtag aaaacgcagc
     1381 acctcgagcg cattgtcgtc gtccttgatg tgcaccatgt cgcggtcgag cttgatccag
     1441 tcgaagggca gctggcgcag gcgcgtcagg ccgctgtggc cggagcccag gtcgtcttgc
     1501 gcgagcttca cgcccaggtc cttgaaccgg gtgaggatcg cgggttggcc gtggctgagc
     1561 gatacggttt cgttttccag gatttccagg gtcagttgtc cgggcgggca atggtgccgc
     1621 gcgagcgcgg cctcggtggc ttcgtaatag cggatgtcgc tcagcccggc gggcgggagg
     1681 ttgatggaca ggtccagctc gatgcccgat ccggtccatc ccgccttgtc ccgcagggcg
     1741 tattccagtc cgcggacgta gatctccagc aggtcgtcga gcaccagcgc cggcaggaag
     1801 tgctccggca acagcagccg ccggccgtcg atcagccggg cgagcgcctc gaccttgcgc
     1861 accttgccgg tatgcgtatc gagcagcggc tggtagtagt tgatcaggga gtccgaacgc
     1921 accagggcgg ccaggtgctg gcggttcttg atcgggatcg tttcgcgcat gccgtctttc
     1981 gcgccgaggc gttcgagcgc gcagcccagc agcgtctgca gcatgtccag gaaagaagcc
     2041 tggtgcgggc cgatgaatcc gccgggcaag gcgctgtgca gcagcaggat ggccaccgtg
     2101 gcgcgccccg gcgcatacag gggaacggcg gcgcaggaac gcaggcccag cttcagggac
     2161 tgctcccgcc acaccgaagc gtgctgctgg gtcttgaagt tgatcatgcg ctcgatatgg
     2221 ccgctgcgcc acgcccggcc caccgggccc tggcctcgcg ggtcgtcggc attgaccatg
     2281 atctgggttt caggggcgcc ttgcctgtcg tccaggtagt cctggatatt gccgcttgcc
     2341 acttcgaagt ggaagacgtt gtgttcgtcg ggcctgccga tggcgcagcc ggccacttcg
     2401 tcgtgctggc tcaggatgtg gacgacctgg tggatcaggt ccgtgtagtt ggcgctttcc
     2461 cagatgacgc gcatgagctc gagcagcgcg gtcttctggg cgtcctcgat ggcctggtag
     2521 gccttgagct gccaggtcag gtcgttttgc agccgctggt tcagcagcga caggtcctgg
     2581 ttgctgacgt tctgggcaat gcgctggtaa accgcggcgg acaggatgct gcggctctgc
     2641 gccagctctt ccttgtccag gccgacgatg gcatggatgc ggcctacgcg cagggcggtg
     2701 gcggcgtggt cttcgtcctg caggctgggg tccgccatgg acaggagatt ctggacctgg
     2761 cgggacttca ggtgctggag ttcgccggga tccagcatgc cgaggatcac gcgggatttg
     2821 ggcagcagcg agagcttgcc gtagaaatcc tccacggcgg gtccggcgct atcacgcaac
     2881 gcttcgatca ggggtgtcac tgcaattcct gctagggatc gatgtatgcc gcatggcccg
     2941 gactttgggc gccagtcccc ccaacctgct ttggcgccgt gcctttggtg catgcgctca
     3001 ctgtaacatt gcctgtatct gttgggtttt atgtccgcct atgacaattt tgcgtggaat
     3061 tggctcgatc aatggactgg ccggcgcgtt gcttgtcgca ctggccgtcg gcgcgcgggc
     3121 ggcgcccgtc accgcgcaag cctgcgactg gcctgactgg aacattttca aaagcacggc
     3181 gatgagcgcc gatgggcgcg tcaccgatgc gagcgagaac ggcatcacga cctccgaagg
     3241 ccagagctat gggctgttct tcgcgctggt ggcaaacgac cgcgcaagct tcgacaagct
     3301 gctggcctgg actgaaaaca agctggctgg cggcgatctg gctgcccgcc tgcctgcctg
     3361 gctgtggggg cggcggtccg ctggccagcc ggcggactgg gatgtgctcg acaccaactc
     3421 ggccagcgat gccgacctgt ggatcgccta taccttgctc gaggccggcc gcctctggca
     3481 tgagcgccgc tatagggcgc tcggctcgat catggcgcgc gcgatcgtcc gccaggaaac
     3541 cgcgcaactg cccggtctgg gccgtaccct gctgcccggc gccgacggct ttcatccggc
     3601 tcccgatctc tggcgcctga atcccagcta tgtgccgctg caactgatgc gcggcttggc
     3661 gagcgcgctg ccgcaccagg ccgaatggaa agccctggtg gatacgtcgg cccatctggt
     3721 caacgacacg gcgccgcgcg gcttttcgcc ggactgggtc ctgtatcgca gtggccatgg
     3781 tttcgagccc gatgtccaga cccgggcgga aagcgcctac aacgccgtcc gcgtctacct
     3841 gtgggccggc atgctcgcgc cggacgagcc cctgcgcgcg gcgacgttgc gcaccttcgc
     3901 gccgctggcc gactatgtct ccgccaacgg tttcccgccc gagcggatcg acacccggac
     3961 aggcgagtcc ggcccgaatg ccggcaatgc gggtttttcg gcggccgtgg cgccgtactt
     4021 ggccgcgctg ggccgcgacg acctggcccg cgcccagcag gagcgggccc gcgccgccgc
     4081 cgcgaacgcg ccgctgggct attacagcag cgtgctgacc ctgttcggta tcggttatct
     4141 ggaaggccgc tatcgcttcg acgcgggcgg ccgcctcgtg cccgcatgga ccgcgacatg
     4201 tcccggccgc tgaccccgcc gctttgcata tcggcgtaga gaaagacgga ctccgggccg
     4261 ggcacgccgg cggccggtgt cgatggcctg ctgtaagatc gcccgacgac gatcaatcga
     4321 ggggcgacat gggcgtagtg ctggcatgcg atctgggcgg caccaccttg cgggtggcgc
     4381 tggtcgatgc gcaaggcgcc gtgctggccg atgccatggt cgacagcccc gccggttcat
     4441 ccggagaagg ctgggccgag atcgacgccg ccgactggtg gcgcgcctat gccgaggcgg
     4501 tcgaggccct ggcgcaaaac gcgggcgcgc tgttcgacgc ggccgaggcc ctggcgacct
     4561 gcggcgtgac gcgcacccag gtctttctcg atgcggccgg ccgggccttg cgcccggcca
     4621 ttacctggcg cgatgcccgg gcggcggatg tgatggacga ggtcctggcg gccgtgccgc
     4681 ccggccatgc cgaacgcgcc gcgctcaacc cgttccatcc cctggcgcgc ctggcctggc
     4741 tgcgccggca tgagccggcc gcccacgcgc ggctgcggtc ggtggccgag cccaaggact
     4801 atctcaatct gcgcctgacc ggcgttttgg cgaccgacga gatctcgtcg gcccggctgg
     4861 ccgccgtgcg aggcgcgccg ctggcggccg tggggtgcgc cgacgccgtg ctgcccgcgg
     4921 tgctgccgcc cgaaggcgag gtggggcgcg tcgcggcggg cctgcccggc gcgctggggc
     4981 ggctggcggg caggccggtc tacgcctgcg cccatgacac ctgggccgcc gtcgtggggc
     5041 tgggcgccct gcgccccggc catgcctaca acatttcggg aacgaccgag gtgttcgggg
     5101 tgatcggcac gcagccggcg caggcgccgg gcttgatgac ggtggattgg ggggccgggc
     5161 tccaccagat aggcggaccg ggccagaacg gcgccgatac cgtggcctgg ctgctgcagc
     5221 tgcttgccag gcaggcgcag ggcaagaccg acgtggccgg aacgctggac gcgctcctgg
     5281 cccggccgcg cgacccgcag cccctgctgt tcctgcccta cctgcaaggc gagcgcgtgc
     5341 cgtattggga cgccggcctg cgcggcgcct tcgtcggcct gaaccggcgg cacggagcgg
     5401 gcgacctggc ctgggccgtg ctggaaggcg tggccttcct gaaccggatc gtgctcgagc
     5461 gcggcgagca ggccgcgggc agcccggtgt cggagatcca tttcggcggc ggggccgcgg
     5521 ccaacccggt gtggtgccag gtcaaggccg acgtgtgcga gcgcccgctg gtggtgggcg
     5581 cccaggcgca gccgggcatc gtgggcgcgg ccgcggtcgc ctggacgggg ctgggccgtt
     5641 atgcctcgct ggacgcggcg cagcaggcgc tggcccgtcc cagccggcgc cacgagccgg
     5701 atgccgcgcg cagcgcggcc tatcggcgtc ttttcgaact gtaccggcgc gcggaagatg
     5761 ccctcgcgcc gatttcacgg gagctggccg cgcatgctgc gtaatgtcct cgagcgctac
     5821 ggaaccgccc tggcgggcct ggtgctggtg ttgttctttg cctggacggc gcccaatttc
     5881 gcctcggcgg gcaacctgct caacatcctg aaggaaacca gcttcctggc catcctgtcg
     5941 ctgggcttca ccctggccct gatcaatgcc gagctggacc tgtcggtggc cgacgtggcc
     6001 agcctggccg ccgtcgtctg cggcgccctg atccacggcg gccagccggt gccgctggcc
     6061 gtggcgggcg gcctgggcgc gggcctgggc tgcggcatca tcaacggcct ggtggtgacc
     6121 cgcctgcgcg tgccgtcgct gatcgcgacg ctgggcatgg cggccatggc gcgcggcttc
     6181 ggcttcatgc tgaccgaggg cgtgtccttc gtggggcgct ggccgcagtc cttcaccggc
     6241 ctgggccggg gcgtgctgct gggcatcccg gcgctggtgt ggtggctggc ggccgtggcg
     6301 ctgggttcgc tcttcctgat caagtgcacg cgcacggggg cccgcatgac ggccacgggc
     6361 gaggccagcg aggcggcgcg cctggccggc atcaatacgc tgcgcatgaa gggcctgggc
     6421 ctggcgctgg ccggcctgct ggccgggctg acggccgtgc tgctggcggc cagcctgtcc
     6481 tcggccgcgc ccaatatggc gggcgactac ttcctgtatg ccatcgcggc ggtcctgctg
     6541 ggcatgacca tgttcgagcc cggccgcgcc aacgtgcccg gcacgctggc ggccgcgctg
     6601 acgctcaagg tgctgggcaa tgggctggtg ctgctgggcg cgccgtatta tgtgcaggac
     6661 attgtcctgg gtttcatcat catcgtttcc gtggcggtat cggccgcggt actgaaaaaa
     6721 gccgctttca agttctgacg acaaggaccc caccatgcgt aaacgctcca aacacctgat
     6781 cgccgccctg gccgccgcgc cgctgctgct ggccggcgcc gccgcccacg ccttcgaact
     6841 gggcatcgtc gccttccaga tgtcgtccga gacgcacgcg cgggtggcca atgccgccgc
     6901 cgccgaggcc cgcgccaaag gctggaacgt gaccctgctc aattcggagg gcgccctgcc
     6961 caagcatgcc gagcagctcg agacgctcat caacaagaag gtcgacgcca tcatcgtggc
     7021 catgggcaag ccggtcgagg ccgaggccca gctcaaggag gcccaggacc acggcattcc
     7081 cgtgatcggc gtgatgtcgg gcgcgagccc ctacatgctg ttcgacgtcg agaccaatga
     7141 atacaaggtg ggcgccgagt cggcgctgta cctgctgggc cagctgggct accagggcgg
     7201 cgtcctggag tcgcgcttcg acggcaattc gggcacgcgc atccgcggca aggtgctcga
     7261 cgccgtgctg tccgaaaacc aggcggtcaa ggacgtcggc aatttctcga tggcccgcac
     7321 gcagagctgg caggacgacg tgcgcaacgg catgcgcgcg ctcatcatgc agaacaccgg
     7381 caagttcaag ggcatctggg tctcgttcga cggccaggcc tatgtcatcg acgatctgct
     7441 gcagcagcaa ggcatgaaga agggcgacgt ggcgctggtg tcgaccgacg gcggggcgga
     7501 aacctatcgc cgcatcgccg atccgcagag cctgatcacg gccaccgtga tgatcccctt
     7561 cgagaccatg ggcaagacgg ccgtggactc cctggacaag atcgtggtgc agaaacagcc
     7621 cagggaaagc gtgacggcgg gtccctacct gttcgccgac gccgtgctgg tcgacaagaa
     7681 caacgtgcag cagttcctca agaaagaatg accggccgca cgcccatcct ctcgctgcgc
     7741 caggtgcgca agtcgtacgg cgcggtcgag gccctgcgcg gcgtcgacct ggacgtgcat
     7801 cgcggcgagg tgctggccat ctgcggcgac aacggcgccg gcaagtcttc gctgatccgc
     7861 gtcatttcgg gggcgcaggc ggcgtcgggc ggcgagatcc gcctcaaggg cgaggtcgtg
     7921 cgcctgcgtt cgccgcagga cgcgctggcg cgcggcatcg ccaccatcta ccaggacctg
     7981 gcgctggcgc cgcgcctgtc gatcgccgag aacgtcttca tggggtcgga gctggtgcgc
     8041 cgcgtgctgc ctggcgtgcg ggtgctggac aggcgcgcca tggccgagca ggcgcgcggc
     8101 tacctgcagc gcctgtcgct ggcggtggac gacatgcgcc gtccggtcga gcgcatgtcg
     8161 ggcggccagc gccaggccgt ggccatcgcg cgcgcgctgc gctgggatgc cgaagtcatc
     8221 atcatggacg aacccacggc ggcgctgggc gtgaaagaaa ccgcgctggt gctggggctg
     8281 gtgcgcacct tgcgcgaaga agggcgcacg gtgatcctga tcagccacaa catggccgac
     8341 gtggtggccc tggccgaccg cgtggccatc ctcaagggcg gcgtgaaggt ggccgagcgc
     8401 gaggtggccg ggctggacgc cgatcgcctg gcgcacatgg tgatgacggg cagggacgcg
     8461 gccgtggccg cgtgagggct tgattgccct cgctggcgct caagccggtt ttctatggcg
     8521 cttgacgtag tttcgcagta cgccgtccat gcgggattgc cagccatcac cggtcgcgcg
     8581 gaaaaaatcc accacttcct ggctgtaccg cacggatagc agcaccttgg gcgaagccga
     8641 gcgcggccgt ccacctttgt tgaccacggc gcgggtcatc atttcgtccg tcagctcggg
     8701 cagttcgtca tattcgtgtt cggcaatgat gtgggcatcc accttgcgca tgtcagattg
     8761 caaggagtgg gccgaggcgg tttttctcac ggtcattggt ttttctcatg ctgaagatat
     8821 gaaggctatc gccacgagcg acatacccaa cgacgatcat gcgccctgcc agcatgccat
     8881 agcagaggat gcgtttttct ccatagtcgc gcctggtgtc ttcgatttga agggtggctc
     8941 cttcaaacgc aagaaccgca tcttcaaaat ccaggccgcg ctcggcaagc gtcgcatttc
     9001 gcttagcttg atcatacgtg gttgggaaag ctacagtcgt gtgatttgtt ggtccgctgt
     9061 ttgcttgagc agatcggaga tgtcgcctga cggcagcgcc aagtgcggcg cgaggtcgtg
     9121 cagcgggcgc agcacgaagg cccgttcatg catgcgcggg tgcggcaggg tcagccgttc
     9181 ggtcgccatg gcgacattgc cgtacaacag caggtccagg tccaaggtgc gcggcgcgtt
     9241 gcggtagggc cgctgccggc cgtgggccag ctcgatggcc tgcagcgcgt cgagcaggtc
     9301 cagcggcgcc agggtggtgt ccagcgccgc gacggcgttg atgaaatcgg ggccgctcgc
     9361 atcgaccggc gcgctgcggt agaaaggcga ggccgcgcag gcctgtatgc cgggcagggc
     9421 ggcgatgtcc gctagggcgg tgcgcagggt ggtgccgctg tctcccaggt tggaacccag
     9481 gccgatgtag gcacgcacgg ttttattcct cgtccggcag gctgggctcg ccgccggcgg
     9541 cgccggccgc gcccgcgccc gccggcttgc ggcgcggccg gcggcggcgc ttgcgggccg
     9601 gcggcgcgtc gccggcctcg aacggcaggt tcgcggtgtc ctcgatcatc ccggcgcggg
     9661 tttcgtcgtt ggcgttggcc aggtccatcc accattgcgc cagcacgctg tcgaattcgc
     9721 ccgcggcggc gcgcagctgc aggaagtcgc acgcggcgcg aaagcgcggc tgctcgatca
     9781 tgcggtagat ggtcttgccc tggcggcgct cgaagcgcgg ctgcatgaac cagatctcgc
     9841 gcatgtccga cgagaagcgg cgctggatgg cgagcttctc ggtctgctcg tcgagcaccg
     9901 agtcggcggc ctgccccagc gcgggaatgg cgctttcgcc gtcggccacg agctgcttcc
     9961 agcggctttc gacctgcggc cacagcaggg cggcgaacag gaagctgggg ctgatgccgc
    10021 ggccggaacg cacgcgggcg tcggtgcggt ccagcgcctc ttcgatgaac ttgcgcccgc
    10081 cgggctgctc cagcacgatg tccagcaggg gcagcatgcc gtggtgcagg ccttcgtcgc
    10141 gcaactgctt gaggcagtcg atggcgtggc cgcaggtcag cagcttgagc atttcgtcga
    10201 acaggcgcga ggccggcacg ttctcgatca ggtcggacat ggtgcggatc ggctcgcgcg
    10261 tggccgtgtc gatggtgccg ttgagcttgg ccgcgaaacg caccgcgcgc agcatgcgca
    10321 ccgggtcttc gcggtagcgc tgcaccgggt cgccgatcat gcggatctgg cgttttttca
    10381 ggtcctgcac gccgttgtgg tagtcgatga cttcttcggt atgcgggtcg tagtagagcg
    10441 cgttgatggt gaagtcgcgc cgcgcggcgt cctgctcctg cgtgccgaac acgttgtcgc
    10501 tgaggatgcg gccgtgctcg tcggtcttct gctcgccatt ggccggcgcg cggaaggtgg
    10561 aggtttcgat gatttcctgg ccgaacacca cgtgcaccag ctggaagcgc cggccgatga
    10621 tgcgggcgcg gcggaacagg gcgcggattt cttcgggggt ggcattggtg gccacgtcga
    10681 agtccttggg ctcgaggccg aggatcaggt cgcgcacggc gccgcccacg acataggccg
    10741 catagccgtg ttgctgcaat acctcgcaga ctttcacggc atggcgcgag acgttgcgcc
    10801 ggtcgatgcc gtgctggtcg cggccgatgc gctgcgggcc gctggcggtc ggcgtgaaca
    10861 ggcggccgac gaatttcttg atggtggttg tgatcatttc agtttctgcc cggccgcccg
    10921 aaggaggctg aagccccctt ggggggcagc gagcgtagcg agcgtggggg tggtcattac
    10981 gacttggcgt cctgtgcgtg gtcgaacagg tcgagcactt gccagccctg gcgttcggcg
    11041 agggttcgca gggctgggct gggattggtg gcgatgggcc gggtgacgcg ctccagcagg
    11101 ggaacgtcgt tgatggagtc gctgtagaag aacgagatat cgaagtccgc catggccaga
    11161 cctatgccgg acagccattg ttgcacgcgg acgactttgc cttccttgaa gctgggcgtg
    11221 ccggcgatgc ggccggtata gcggccggcc acgtattccg gttcggtgcc gatgaggtgg
    11281 ggcacgccga aggcgcgcgc gatgggggcg gtgacgaagg tgttggtggc ggtgacgatg
    11341 gcgcagaggt cgccggcctc gaggtgctgg cgcaccaggt cgagcgccac ggactggatg
    11401 gcgggccgca ccacctcgcg catgtattcc tcgtgccaca gcgccagttc gtaggggctg
    11461 tgggcggaca gcaggcccag catgaactcg gcggcctgct cggccgtcag gtcgccgcgg
    11521 ttgtagcgtt ccatcaggtc gttgttgcgg cgcagggctt ccccggggtc gccggcacgg
    11581 ccggtgcgcg ccaggaactc cgcccactgg tggtcgctgt cgagcggcag cagcgtgtgg
    11641 tccaggtcga acagggcaag gcgtcgggca gcggtcatgg tgtattcgat tcagggtccg
    11701 cgagcatggc gcgcagcagc gggatggtga tggctcggcg cgtcgccagc gaatagcggt
    11761 ccagcgcatc gagcagggcg gtcagcttgc ggatgtcgcg ctcgtggtga gtcagcatcc
    11821 agttgagcac ttccgcagac agttgcaggc cccgctcggc ggcctgggac gacagggccg
    11881 ccagcttgtc ggcgtcggac aggggatcca ggcggaacac cagcccccag cccagccggg
    11941 tgcgcaggtc ttcgcgcaag ggcatggcca gcggcgcgcg gtcgcccgcg acggccaggg
    12001 cgaaggcccg gccggcagcc gcggattctc gccagcgatt gtacaaggcg aagagcgccg
    12061 cctgccgggc ctcgtccatg cggtgcacgt cgtcgacggc caccagggca gggaggtcgg
    12121 ccgcggcatc ggcctgggcc agggccagga aggcccgggc gtcctgggcg gcgtccagat
    12181 agacgccgcc ggagcgggcg gccaggccgc gcagcaggtg gctgcggccg cagccggccg
    12241 ggccccatag atagagggcc tggccggccg tcaggccgcg ggcggcggtc agggcctcgc
    12301 cgttggcgcc ggcgatatag ttgcccaggg tcggggcggg ttcgggaagg acgtccaata
    12361 ggagctggcg gttcatcgca aacagctttc aaatgcggcg catttaaagt cgggtatcaa
    12421 atcgaaagta tcgaggccga ggggattaaa gtcgattcac cggcggggat cggcggggtt
    12481 gtaaaatccc gaccattcat ccaaatcccc taattgttac ataggccacg gtttttctca
    12541 tgacgaatcc cacctccgct cccctgactt accgcgatgc cggggtcgac atcgacgcgg
    12601 gcgacgcgct ggtcgatcgc atcaagccgc tggccgcgcg caccatgcgc cccggcgtgc
    12661 tggccggcat cggcggcttc ggcgccctgt tcgaggtgcc caagaagtac aaggaaccgg
    12721 tgctggtgtc gggaacggat ggtgtcggca ccaagctgcg cctggccttc gagtggaatc
    12781 gccatgacac ggtgggcatc gacctggtgg ccatgagcgt caacgacatc ctggtgcagg
    12841 gcgccgagcc gctgttcttc ctggattatt tcgcctgcgg caagctggcc gtggacacgg
    12901 cggcctcggt ggtgggcggc atcgcgcgcg gctgcgaact ggcgggctgc gccctgatcg
    12961 gcggcgagac ggccgagatg ccgggcatgt accccgaggg cgagtacgac ctggccggtt
    13021 tcgcggtggg cgcggtggac aagccggcca tcatcgacgg caagtccatc cggcccggcg
    13081 acgtggtgct gggcctggct tcgagcggcg cgcattccaa cggctattcg ctggtgcgca
    13141 agatcctcga gcgcgcgggc gcccgccccg agcaggactt ccatggccag aaactggtgg
    13201 acgtggtgat ggcgccgact cgcatctatg tgaagcaggt gctggccgcg ctggagaagc
    13261 acggcccggc gatcaagggg ctggcgcaca tcacgggcgg cggcctgctg gacaatgtgc
    13321 cgcgcatcct gcagcccggc ctgtcggcca ggctgcaccg cgatgcctgg gagctgcccc
    13381 tgctcttcca gtggctgcag cagcagggcg gcgtggccga tggcgagatg caccgggtgt
    13441 tcaactgcgg gatcgggatg gtgatcgtgg tgccgccggt ggcggccgac gcgatcgccg
    13501 cgaccctgcg cgagcagggc gagacggtct acgcgctggg cgagatcgtg ccgcaggcgg
    13561 acgggatggc gcagaccgtg gtggtgtgac gtcagcgcat gtaggcgccg ccattgacgt
    13621 ccagcgttgc gccggtcatg aaggcggaga ggtccgacgc caggtatagg caggccccgg
    13681 cgatgtccgc cggctggccg acgcgggcca gcggaaattc ccgggcccac tcgtgcttgt
    13741 tttggtcgga attggcgccg cgcgaaaagt cggtcaggat gagccccggc gtgacggcat
    13801 tggagcgcac gcccttgggg ccgaattcct tggcgatcgc gcgcgacagg ccgagcacgc
    13861 cggccttggc cgcgcagtag tgtccgccgc cgaagacgcc gccgccttgc tgggccgaca
    13921 tcgacgagat gtggatgagc gccccgccgc cctgctcgat cataggtgga atcacgctct
    13981 gggacatttg cagcgcgccc ttgaggatga tgccggtgac gcgttcgtag tccgcgccgg
    14041 tgatgtccag gatgcccgcg cgctgggtga cgccagcatt gttgaccagc gcgtcgatgc
    14101 gcttggccca gtccagcacc cgggccacgg cggcgcggca ggccgccggg tcggacacgt
    14161 cgcaaccgac gcccagatgc tccgggccca cggagcgagc ggcctgggcg gcttcgccct
    14221 cgtcgaggtc gagcagcgcg acgcgcgcgc catgctcggc gaacaggcgc gcggtcgcca
    14281 gcccgatgcc gcgctcgcgc gaggcgccgg tgaccaccgc aacgcggcct tcgagcagtt
    14341 ttccggacat tttcatgggt ttgtctcctt atggatcttg atggattcag gcacggtccg
    14401 ggcgcattgc gcgcgtccta gaccctcgcg tccgttctgg tccggacggc aagcggcggc
    14461 ggcatgcagg attcgcgcag gatcatctgc gtcgtgatca ggatgcgttc gggttctttc
    14521 tcctcgcgcg ccggcaggtt gcgcagcagc acttcggcgc aggccgtgcc cagggccgcc
    14581 aagtcccagg tcagggacgt gatggcgggc gtgaaaagct gcgacagatc ggtatcgccg
    14641 atactgatga cgctgacgtc gtccggcacg gtgcggcccg aatggcggat ggcctgcagg
    14701 acgcccgaca ggatgcgcgt gcccaggcaa aggaacgccg tcggctcgtt ggccgaggac
    14761 agcagcgcca gggcttcgct gaaagagaac tccatggatg agctttcggc gcggatgagc
    14821 cgggggtcgg gctgcacgcc ttgcgcggcg aaagcgtcct cgaagcccgc gatgcgttcg
    14881 cgccctgggc gcagggcgct gcccggcgtg agcagggcaa tgcggcggtg gcccagcgta
    14941 agcagatagc gcgtggcttg caaggccccg tggtaatgat ccacatgcac gcccgtgccc
    15001 tcggccgtga tgtcccggtc gagcgccacg acgggaacgt cggaggcgag ccgctccagc
    15061 atctcgggct tttccgagtc gcaagggccg aggatgaggc cgtcgacgcg tctgcggcgg
    15121 aacaggtcga tcagcgattt ctcccggttc gcatcgccat gcgtgctggc cagcagcagc
    15181 gtgtagcccg ccgagaacag ccgcgcctcg acggcggtga tgatcccggc ataaagcggg
    15241 ttggagatct cggacaccag cagccccacg acgccggtgg aacgcgtgcg catgctctgc
    15301 gccaaggcgt tgggcacata gcccagttcc cgcgcgacac gggataccgc ggcgatggct
    15361 tgtgtgctca cggggccggt gcggttcagc gcgcgcgagg cggtcccgag cgatacgccg
    15421 gcg
//
LOCUS       DASWPO010000033         8015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2328068, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000033 DASWPO010000000
VERSION     DASWPO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328068"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VGC69_01665"
     CDS             complement(<1..131)
                     /locus_tag="VGC69_01665"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY0294021.1"
                     /translation="MPSSLSLIVAYASNRVIGRDNDLPWKLRGDLAHFKRTTLGHPI"
     gene            complement(158..1129)
                     /locus_tag="VGC69_01670"
     CDS             complement(158..1129)
                     /locus_tag="VGC69_01670"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY0294022.1"
                     /translation="MKQYLDLVRTIIDQGSWQENRTGVRTLSLPGAALRFDLQQGFPA
                     VTTKRLAFKSAIGELVGFMRATRSAAEFRELGCKVWDQNANENAQWLANPYREGPDDL
                     GPVYGVQWRQWPAYKSLPAAREKQIADALSRGYAKVADIIERGQPHVLLYKAVDQLRQ
                     CLDTIIERPGDRRILFHGWNWAQLEEMALPPCHLLYQFLPNAGTREISLCLYIRSNDV
                     GLGTPFNLTEGAALLHLVGRLTGYTPRWFSYFIGDAHIYENHLPMLQEQLTRAPYAAP
                     RLQLSDRIPDHAVTGKYEPDWLERVEPSDFVLENYQHHAPLTAPMAV"
     gene            complement(1179..1604)
                     /locus_tag="VGC69_01675"
     CDS             complement(1179..1604)
                     /locus_tag="VGC69_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0294023.1"
                     /translation="MECTVEWGGPSGMLFTASTGSGHVAVMDGAVEGGGHDLAPRPME
                     MVLLGTGGCTAYDVVLILKRGRHVVTGCSVKLEAERAATDPKVFTRINFAFTVTGRSL
                     PRDAVERAVELSHEKYCSASAMLAKTAELSFTVDIAEAP"
     gene            1791..2483
                     /gene="coq7"
                     /locus_tag="VGC69_01680"
     CDS             1791..2483
                     /gene="coq7"
                     /locus_tag="VGC69_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY0294024.1"
                     /translation="MPSPFTQILAKLTLPSLPGAGPGFIDGLLDEAGRALRVLSGSAS
                     AGRPYPARAEEPAGQLSDQDKRHAAGLMRVNHVGEVCAQALYRGQAVGCRDASTRTLL
                     RGAAAEEVDHLVWCGERLDELGSRPSLLNPLWYAGSFALGVLASYAGVPRNLGFMAET
                     ERQVEAHLESHLRTLPVRDQRSRQVVVQMKNDEAGHRDSAEQAGGVPLPAPVRGAMRA
                     MSKVMTGTAYWI"
     gene            complement(3267..4832)
                     /gene="pgi"
                     /locus_tag="VGC69_01685"
     CDS             complement(3267..4832)
                     /gene="pgi"
                     /locus_tag="VGC69_01685"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY0294025.1"
                     /translation="MSASLPSSPAWQRFAQAARDASLRGEPLRIIDAAGLQIDLTTQR
                     HSTELDQACAALLAQQGFEKTRAGLFSGEPINWTEHRAAWHTALRAAQPPAAVAEQVL
                     GERQRVRQFVADANAHGAWKHVLHLGIGGSDWGPRMVVRALNGNGLQRTLRFAANVDA
                     HDILLQLDDLDPAQTLVIVASKTFTTTESLANAQVALDWLRQAGIADPLSHVAAVTAN
                     PQAARDFGVSDKHIFRFWDWVGGRYSLWSAIHLPIALALGNDTVDELLAGAEAMDQHF
                     LHAPLAANAPVQMALSVVANRSVLGYPTQAIAPYDSRLSYLVPWAQQLEMESLGKSAT
                     ADGTPPGVPTSPVVWGMTGTDSQHTFFQWMHQDAQGAPVDFIVCREADHGYERHHRIL
                     LANCLAQRAALLTGKSYDEALLETQRTESDPAQAALLAHHRVHPGGRPSTLIMLPRLT
                     AGTLGALLALYEHKVFTQGVLWGINTFDQWGVEFGKVLAKRIIGELDAPDGGAGSWQD
                     ASTRHWIGLLGKQ"
     gene            complement(4838..6232)
                     /locus_tag="VGC69_01690"
     CDS             complement(4838..6232)
                     /locus_tag="VGC69_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY0294026.1"
                     /translation="MSATPFPASVFKAYDIRGTVPDLINPDFARALGTALAARARKAG
                     IAAVVVGRDGRLSSPMLAQAMVEGLTAAGVDALDIGQVPTPLVYYASYTQGTGTGVAI
                     TGSHNPPKYNGFKMMLGGKALYGDEIKQLPAEMAEAGSGKLAAATPGKHIKLDLVGEY
                     IDRIVSDVKLARRMKIAIDAGNGVAGAVAPALLRALGCEVDELYTEVDGNFPNHHPDP
                     AEPHNLEDLIKHVTTTNCELGLAFDGDGDRLGVVTKSGEIIWPDRQLILLARDVLTRV
                     PGGTIIYDVKCSRHVGLEVEKAGGKPLMWMTGHSLVKAKLAETGAPLAGEMSGHIFFK
                     ERWFGFDDGLYTAARLLEIVSRSANPSQVLEDLPQALSTPELKIEMEEGQPHAIIRDL
                     QASGSFPGTTRVVTIDGVRAEYPDGFGLARPSNTTPVIVLRFEADNQAALTRIQDDFR
                     RELLKLAPQAKLPF"
     gene            6485..7900
                     /locus_tag="VGC69_01695"
     CDS             6485..7900
                     /locus_tag="VGC69_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0294027.1"
                     /translation="MTLLNELQSLLSVDHVLTGADAAPFLIDWRRRYQGSALAVARPG
                     STDEVAAVVRACVRHGVPLVPQGGNTGLCGAATPDETGNAVVLSTARLNRVRSIDTDN
                     DTITVEAGCVLQAVQEAAEGAGRLFPLSLAAEGSCTIGGNLATNAGGTQVLRYGNTRE
                     LTLGLEVVTAEGEIWHGLRGLRKDNTGYDLRDLYVGSEGTLGVITAATLKLYPLPVAT
                     CTALLALRSIDAAVDLLARARTGFGASLTGYELMAGDCLQLVVELFPQQRLPFEGESA
                     QAPWYALLELSDSESETHARDRFEAVLGQAIEDGVVTDAAIAANMAQSKALWHLRESI
                     PLAEAEVGKSVKHDVSIPISSIADFVHTTNAKLQSGFAGLRNVIFGHLGDGNLHYNVS
                     RNAGLSQDELLALQPRIYDLVHASVQSYGGSISAEHGIGQLKRDELPHYKSPLELALM
                     KRIKRALDPQGLLNPGKVLQM"
BASE COUNT         1290 a         2526 c         2842 g         1357 t
ORIGIN      
        1 atgatggggt ggcccagggt ggtgcgcttg aagtgggcca ggtcgccccg cagtttccag
       61 ggcaggtcgt tgtcgcgccc gatgacgcgg ttgctggcgt aggcgacgat cagcgagagg
      121 ctggacggca tgtggcgcgc ggttcgatgt aaagggttca gaccgccatg ggagcggtca
      181 gcggcgcatg atgctggtag ttctccagca cgaagtccga gggttccacg cgctccagcc
      241 agtcgggctc gtacttgccg gtgacggcat ggtcggggat gcggtccgac agttgcaggc
      301 gcggcgcggc gtagggcgcg cgcgtgagct gctcctgcag catgggcagg tggttttcgt
      361 agatgtgcgc gtcgccgatg aagtacgaga accagcgcgg cgtgtagccg gtaagccggc
      421 ccaccaggtg cagcagtgcc gcgccttcgg tgaggttgaa gggcgtgccc aggcccacgt
      481 cgttgctgcg gatgtacagg cacagcgaga tctcgcgcgt gccggcgttg ggcaggaact
      541 ggtagagcag gtggcagggc ggcagcgcca tttcctcgag ctgcgcccag ttccagccgt
      601 ggaacaggat gcgccggtcg ccgggccgct cgatgatggt gtccaggcac tggcgcagct
      661 ggtccacggc cttgtagagc agcacgtgcg gctggccgcg ctcgatgatg tcggccacct
      721 tggcgtagcc gcgcgacagg gcgtcggcaa tctgcttttc ccgcgcggcg ggcagcgact
      781 tgtaggccgg ccactggcgc cattgcacgc cgtagaccgg gcccaggtca tcggggcctt
      841 cgcgataggg gttggccagc cattgcgcgt tctcgttggc gttctggtcc cagaccttgc
      901 agcccagctc gcggaattcg gccgcgctgc gcgtcgcgcg catgaaaccc accagttcgc
      961 cgatggccga cttgaaggcc agccgcttgg tggtgacggc ggggaagcct tgctgcaagt
     1021 cgaagcgcag cgcggcgccc ggcagcgaca gggtgcggac gccggtgcgg ttttcctgcc
     1081 aactgccctg gtcgatgatg gtgcggacga ggtctaggta ctgtttcatc gggcgcgatg
     1141 catgtgggcc aagggaaggg cgcggcgaag gcgcggtgtc agggcgcttc ggcgatgtcg
     1201 accgtgaagc tcagctcggc ggtcttggcc agcatggccg aggccgagca gtatttctcg
     1261 tgcgacagtt ccacggcgcg ctcgacggcg tcgcgcggca ggctgcggcc ggtgacggtg
     1321 aaggcgaaat tgatgcgcgt gaacaccttg gggtcggtgg cggcgcgttc ggcctcgagc
     1381 ttgacgctgc agccggtcac gacgtggcgg ccgcgcttga ggatgagcac cacgtcgtag
     1441 gccgtgcagc cgccggtgcc gagcagcacc atttccatgg ggcgcggggc caggtcgtgg
     1501 ccgccgccct cgacggcgcc gtccatgacg gccacgtggc cgctacccgt gctggcggta
     1561 aagagcatgc ccgaggggcc gccccattcg actgtgcatt ccatagtgat tcctttcgcg
     1621 tttttgagcc gccgcggctg ccctgcggga actccgatgc tcgcccctga aaaggggctg
     1681 cgcttcggat tccctccggg gtccgcggct cacgatattt gccgttggag atcgatatta
     1741 ccccgttgcg taaaatcgac ggttgagtac ttatgccata gatcgagatc atgccgtcac
     1801 cgtttaccca gatccttgcc aagctgaccc tgccgtcctt gcccggcgcg ggccccggtt
     1861 tcattgacgg cctgctggac gaggccggcc gcgccctgcg ggtgctgtcc gggtcggcgt
     1921 cggccgggcg gccctatccg gcccgggccg aggagcctgc cggccagctg tccgaccagg
     1981 acaagcgcca tgccgccggc ctgatgcggg tgaaccatgt gggcgaggtc tgcgcccagg
     2041 cgctgtaccg cggccaggcc gtgggctgtc gcgacgcctc cacccggacc ctgctgcggg
     2101 gggccgcggc ggaggaggtc gaccatctgg tctggtgcgg cgagcgcctg gacgagctgg
     2161 gcagccggcc cagcctgctc aacccgctct ggtatgccgg gtccttcgcc ctgggcgtgc
     2221 tggccagcta tgccggcgtg ccgcgcaacc tgggcttcat ggccgagacc gaacgccagg
     2281 tcgaggcgca cctggaaagc cacctgcgga ccctgcccgt gcgggaccag cgttcgcgcc
     2341 aggtggtggt gcagatgaag aacgacgagg ccggccaccg cgatagcgcc gagcaggccg
     2401 gcggggtgcc cttgcctgct ccggtgcgcg gcgccatgcg ggccatgtcc aaggtcatga
     2461 cgggcacggc ctactggatt tgagtccggg cctgtccagg caaaaaatgg tgcattgcgg
     2521 cggggacggc gtgtagtttt gcgacgcggc acggttagga cgcggcacgg aaagcctata
     2581 tccccagtgg gggaaatcac ctcaaaactc aagggtttac ccttgaattg caagcagatt
     2641 ttcggagaat cgattgggat ttcaaagatt tgcaaaaatc cggcaagcta tccggcataa
     2701 aataaccgtc tattgaaccg ggccgcctca ttccaatctg gaatgtgcgt atgcaaaaaa
     2761 aatcttgcac cgcacaagaa attccagtat tattcgtaca tcggatgtcg aaacgcagcc
     2821 ggagcggtta agaagaaacg cctaaccccc tcctgcagtg atcccgaagc tgaccagcaa
     2881 gatgttagcc caggttgatc ccgatatgcc acggttgtct cctccaccct cctcctttgg
     2941 tggatttagc ccgagatctt tcgatctcgg gctttttttt gagccgaaac aatccgtccc
     3001 caataataaa aatgctgaag ccaaatctcg gaccagaagg ttggcaagca gccttccata
     3061 cttctctgcc ttgagccgag gaccccggag caatccgaag cacagcccct tttcaggggc
     3121 gagcatcgga attgcgcagg gcagcctcgg cgtctcaaga acccagataa ccccggcaag
     3181 caaaaaaacc acgaaccagc ctcacggcca gcccgcggta aatacaaaaa atcggtacaa
     3241 aaactacacg ctcaccaagc cgaggttcac tgcttcccca gcagcccgat ccaatgccgc
     3301 gtcgacgcat cctgccacga cccggctccg ccatccggcg catccagctc cccgatgatc
     3361 cgcttggcca gcaccttgcc gaactcaacg ccccattggt cgaaggtatt gatcccccac
     3421 agcaccccct gcgtgaacac cttgtgttca tacagcgcca gcagcgcgcc cagcgtgccg
     3481 gccgtcagcc tgggcagcat gatcagcgtg gaaggccgtc cgcccggatg cacgcgatgg
     3541 tgcgccagca gcgccgcctg ggccggatcg ctctcggtgc gctgggtttc gagcagggcc
     3601 tcgtcgtagg acttgccggt gagcagcgcc gcgcgctggg ccaggcaatt ggccagcagg
     3661 atgcgatggt ggcgctcata gccgtggtcg gcctcgcggc agacgatgaa atccaccggc
     3721 gccccttggg catcctggtg catccattgg aagaaggtgt gctggctgtc ggtgcccgtc
     3781 atgccccaga ccaccgggct ggtgggaacg ccgggcggcg tgccgtcggc ggtggccgat
     3841 ttgcccagcg actccatctc cagttgctgg gcccagggca ccaggtagct caggcgcgaa
     3901 tcgtagggcg cgatggcctg cgtgggataa cccagcacgc tgcggttggc cacgacggac
     3961 agcgccatct gcaccggcgc attggcggcg aggggcgcgt gcaggaaatg ctggtccatg
     4021 gcttcggcgc cggccagcag ctcgtccacg gtgtcgttgc ccagcgccag ggcgatgggc
     4081 aggtggatgg ccgaccagag cgaatagcgg ccgcccaccc agtcccagaa gcggaagatg
     4141 tgcttgtcgg acacgccgaa atcgcgcgcg gcctgcgggt tggccgtcac ggccgccaca
     4201 tggctcaagg ggtcggcgat gccggcctgg cgcagccagt cgagcgcgac ctgggcattg
     4261 gccagcgact cggtggtggt gaaggtcttg gaggcgacga tcaccagcgt ctgcgccgga
     4321 tcgagatcgt ccagctgcag caggatgtcg tgcgcgtcga cgttggccgc gaaacgcagg
     4381 gtgcgctgca ggccgttgcc gttcaaggcg cgcacgacca tgcgcgggcc ccagtcgctg
     4441 ccgccgatgc ccaggtgcag cacgtgcttc caggcgccgt gcgcattggc gtcggccacg
     4501 aactgccgca cgcgctggcg ttcgcccagc acctgttcgg ccacggcggc cggcggctgc
     4561 gcggcgcgca gcgccgtatg ccaggcggcg cggtgttccg tccagttgat gggctcgccg
     4621 ctgaacagcc cggcgcgggt tttttcgaaa ccttgctgcg ccagcagggc tgcgcaggcc
     4681 tggtcgagtt ccgttgagtg gcgctgcgtg gtcaggtcga tctgcaggcc ggccgcatcg
     4741 atgatgcgca acggttcgcc gcgcagcgac gcatcgcggg cggcctgggc aaatcgctgc
     4801 caggcggggc tggaaggaag ggaagcggac attgcgatca gaagggcagt ttggcttgcg
     4861 gtgcgagctt gagcagttcg cggcggaagt cgtcctggat gcgggtcagc gcggcctggt
     4921 tgtcggcctc gaagcgcagc acgatcaccg gcgtggtgtt ggacggacgg gccaggccga
     4981 aaccgtcggg gtattcggcg cgcacgccgt cgatggtgac cacgcgcgtg gtgccgggga
     5041 agctgccgct ggcctgcagg tcgcggatga tggcgtgcgg ctggccttct tccatctcga
     5101 tcttgagctc gggcgtggag agggcctgcg gcaggtcttc gagcacctgg gacgggttgg
     5161 cgctgcgcga gacgatctcg agcaggcggg cggccgtgta gaggccgtcg tcgaagccga
     5221 accagcgctc cttgaagaag atgtggccgc tcatctcgcc ggccaggggg gcgccggtct
     5281 cggccagctt ggccttgacg agcgagtggc cggtcatcca catgagcggc ttgccgccgg
     5341 ccttctcgac ctccaggccc acgtggcggc tgcacttgac gtcgtagatg atggtgccgc
     5401 cgggcacgcg cgtgagcacg tcgcgggcca gcaggatcag ttgccgatcc ggccagatga
     5461 tctcgccgga cttggtgacc acgcccagcc ggtcgccgtc gccgtcgaag gccaggccga
     5521 gttcgcagtt ggtggtggtg acgtgcttga tcaggtcttc gaggttgtgc ggttcggccg
     5581 ggtcggggtg atggttgggg aagttgccgt cgacctcggt gtagagctcg tcgacctcgc
     5641 agcccagggc acgcagcagc gcgggggcga ccgcgccggc cacgccgttg ccggcgtcga
     5701 tcgcgatctt catgcggcgc gcgagcttga cgtcggagac gatgcggtcg atgtattcgc
     5761 ccaccaggtc gagcttgatg tgcttgccgg gcgtggcggc ggcgagcttg cccgaacccg
     5821 cttcggccat ttcggcgggc agctgcttga tctcgtcgcc gtacagggcc ttgccgccca
     5881 gcatcatctt gaagccgttg tacttgggcg ggttgtggct gccggtgatg gccacgccgg
     5941 tgccggtgcc ttgcgtatag gatgcgtagt agaccagcgg ggtgggcacc tggccgatgt
     6001 ccagcgcgtc gacgcctgcg gcggtcagtc cttcgaccat ggcctgggcc agcatggggc
     6061 tgctcaggcg gccgtcgcgg ccgaccacga cggccgcaat gccggccttg cgggcgcggg
     6121 cggccagcgc cgtgcccagc gcgcgggcga agtccggatt gatcaggtcg ggaacggtgc
     6181 cccggatgtc gtaggccttg aagacggagg cgggaaaagg cgtggcgctc acgaaaaaat
     6241 ccttgtggaa aaactcgaac cggcacgggc cggtggtcgg gcgacagatc gcggccttgt
     6301 cgcgattatc tctgattcag cgtgtccggg ggaggacggg cgtggcatta ggaaacaggg
     6361 cgtgtccaga attatgggct ttttatgggg caatccggtt accggcccgc gcgggcgggc
     6421 cgtgcgccgg ggcgcggccg ccaccctaca atagacagat ccccttgcat aacgagctgc
     6481 tgccatgacc ttgcttaacg aattgcaatc cctgctgagt gttgaccatg tgttgacggg
     6541 ggcggacgcc gcgccctttc tcatcgactg gcgccggcgc tatcagggct cggccctggc
     6601 ggtggcgcgg ccgggcagca ccgacgaggt ggcggccgtg gtgcgcgcct gcgtccggca
     6661 cggcgtgccg ctggtgccgc agggcggcaa taccggcctg tgcggggcgg ccacgcccga
     6721 tgaaacgggc aacgcggtgg tgctgtccac ggcgcggctg aaccgcgtgc gttccatcga
     6781 caccgacaat gacaccatca cggtcgaggc cggctgcgtg ctgcaggccg tgcaggaagc
     6841 cgccgagggg gcgggccgcc tgttcccgct gagcctggcg gccgaaggca gctgcaccat
     6901 cggcggcaac ctggccacca atgccggcgg cacccaggtg ttgcgctatg gcaatacgcg
     6961 cgaactcacg ctgggcctgg aagtggtgac ggccgagggc gagatctggc acggcctgcg
     7021 cggcctgcgc aaggacaata ccggctacga cttgcgcgac ttgtacgtgg gcagcgaagg
     7081 cacgctgggc gtgatcacgg ccgccacgct caagctctat cccttgccgg tcgcgacctg
     7141 cacggcgctg ctggccctgc gcagcatcga cgccgcggtc gacctgctgg cgcgcgcgcg
     7201 caccggcttc ggcgcgtcgc tgacgggcta cgaactgatg gcgggcgact gcctgcaact
     7261 ggtggtggag ttgttcccgc agcagcgcct gcctttcgag ggcgagtcgg cgcaggcgcc
     7321 ctggtatgcg ctgctggaac tgtccgacag cgaaagcgaa acccatgcgc gcgatcgttt
     7381 cgaggccgtg ctgggccagg ccatcgagga tggcgtggtg acggacgcgg ccatcgccgc
     7441 caatatggcg cagagcaagg ccctgtggca cttgcgcgag agcatcccgc tggccgaggc
     7501 cgaggtgggc aagtcggtca agcacgatgt gtccatcccg atctcgtcca tcgccgactt
     7561 cgtgcacacc accaatgcca agctgcagtc gggctttgcc ggcctacgca atgtgatctt
     7621 cggccacctg ggcgacggca acctgcacta caacgtgtcc cgcaatgcgg gcctgtcgca
     7681 ggacgagctc ctggcgctgc agccgcgcat ctacgatctg gtgcacgcca gcgtgcagtc
     7741 gtacggcggc tcgatcagcg ccgagcatgg catcggccag ctcaagcgcg acgaactgcc
     7801 gcactacaag agtcccctcg agctggcgct gatgaagcgc atcaagcggg cgctcgatcc
     7861 gcagggcctg ctcaatccgg gcaaagtgct gcagatgtaa agggcgaggg cgttcagcct
     7921 ttgagcagcg gcgccatggc gtcgagcacg cgctgcacgg cgccggcatg gcgggccacg
     7981 tagcgcaggc cggcctggcg catggcgtcg cggcc
//
LOCUS       DASWPO010000034        28626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_720973, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000034 DASWPO010000000
VERSION     DASWPO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 28626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28626
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720973"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..2460
                     /locus_tag="VGC69_01700"
     CDS             97..2460
                     /locus_tag="VGC69_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY0294028.1"
                     /translation="MPRISNASPRASRNTRNGPSPLQSRLSALLREARWIFFAALAVW
                     LTLVLATWSPSDPGWSHSVSGGALRNGGGAVGAYLADILLYLFGFSAWWWVILLVHRV
                     RAGYRRLATQLRATGSEPETLPRVHWEQGVGFLLLLAGSLGLEALRLRSRGLHLPGAT
                     EHISGAGGVIGQLLANWLSRSLGFDGGTLALLVLVAVGLSLFLSFSWLSVAERIGGWL
                     EQGIRGIANAYTARQDRRVGEVAQAERSEQVEAKHEKMVVHEPIRIEPAMTVVPPSER
                     AAKEKQQTLFELPEGEANLPELGLLDPPLASQETVSAETIEFTSRLIEKKLADFGVSV
                     VVVAAQAGPVITRYEIEPATGVKGSQIVNLAKDLARALSLVSIRVVETIPGKNLMGLE
                     LPNPKRQMVRLSEILGSQTYHAGQSVLTMALGKDIAGHPVVADLARMPHLLVAGTTGS
                     GKSVGINAMILSLLYKADASQTRLILIDPKMLEMSVYEGIPHLLAPVVTDMRQAANAL
                     NWCVGEMEKRYRLMSKMGVRNLAGYNAKIKDAIKREEPIPNPFSLTPDQPEPLQSMPM
                     IVVVIDELADLMMVVGKKIEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIP
                     TRIAFQVSSKIDSRTILDQMGAEALLGQGDMLYMPPGTGLPVRVHGAFVHDDEVHRVV
                     EALRAQGEPNYIEGLLEGGTEGDSADGASSVTGIGGDAESDPMYDQACEVVLKNRRAS
                     ISLVQRHLRIGYNRAARLLEQMENAGLVSAMQSNGNREILVPAREEA"
     gene            2460..3095
                     /gene="lolA"
                     /locus_tag="VGC69_01705"
     CDS             2460..3095
                     /gene="lolA"
                     /locus_tag="VGC69_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEY0294029.1"
                     /translation="MHCAVRVLGRAAVAVAMACGPMLAWGATAQEQLKVFVSSVQSAT
                     GSFNQATASKQGRNPPAQQGAFAFQRPGKFRWMVQKPYEQLIVSDGKQVYQYDPDLAQ
                     VTVRQVDRAIGTSPAAILFGSGDLEQAFTVTALPDRDGLQWLRAKPRNADAGFAQVDI
                     GMKDNLPARILLLDAFGQTTQVDFSGLQPNAAVPATQFQFTPPQGVDVVKM"
     gene            3217..4563
                     /locus_tag="VGC69_01710"
     CDS             3217..4563
                     /locus_tag="VGC69_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY0294030.1"
                     /translation="MSSDLFAGDPAHRPYVPLAERLRPRTLADVVGQSHLLGPDKPLR
                     VAFASGRPHSMIFWGPPGVGKTTLARLMADGFDAQFIAISAVLGGVKDIRDAVTAAQI
                     AQGQGRRTILFVDEVHRFNKAQQDAFLPYVESGLFTFIGATTENPSFEVNSALLSRAR
                     VYVLQALTPEELALLIDRAVDAMNEGRGGEAQLPVLQITSEAREQLAAWADGDARRLI
                     SAVEVVAESALTAERHIVDAAWLENSLSQNLRRFDKGGEAFYDQISALHKSVRGSDPD
                     ASLYWFCRMIDGGADPLYLARRIVRMAWEDIGLADPRAAQVVNDAAATYERLGSPEGE
                     LALGQAVIYLACAAKSNAGYNAYNAARKYAAEHGSTPVPLHLRNAPTKLMKELGYGHA
                     YRYAHDEPHGYAAGEQYFPDGLETTFYRPTDRGLEAKIQQKLAFLKDLDRQHKKKS"
     gene            4715..6073
                     /gene="serS"
                     /locus_tag="VGC69_01715"
     CDS             4715..6073
                     /gene="serS"
                     /locus_tag="VGC69_01715"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0294031.1"
                     /translation="MLDPTLLRKDLQTVVDRLKTRKVDFDTEAFNALESRRKTVQTET
                     ETLQARRNALAKQIGQLKAKGEDASSVMAESQGIPARLKQLEDDLAAVQAQLNDLLMS
                     VPNLPHASVPVGKDSDGNVEVRRWIPGDPKVNAGPDGNPAGLGFEPRDHVAIGEPLGL
                     DFDLAARLSGARFSFMRGPVARLHRALAQFMLDLQTGEHGYTECYTPYIVNSSTLFGT
                     GQLPKFKDDMFFVTKGGGDDDPKVDEQGRPLVREDQYLISTSEITLTSAVRDTIVPAG
                     GLPMRLTAHTPCFRSEAGSGGRDTRGMIRQHQFDKVEMVQIVEPDTSYDTLEQMVGHA
                     EQVLRRLGLPYRVMLLCTGDMGFGAAKTYDLEVWLPAQNTWREISSVSNCEAFQARRM
                     QARFRNAQNKPEFVHTLNGSGLAVGRALVAVLENYQQADGSVVVPEALRPYMGGAEVL
                     KP"
     gene            6201..6446
                     /locus_tag="VGC69_01720"
     CDS             6201..6446
                     /locus_tag="VGC69_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294032.1"
                     /translation="MTMRTIGDFRVRCTIVQENPRQFSVQVCTRKVGGNAPEKCWDVP
                     GLAFDSRAAAEQGSLQVFERINGVRFNGEPEFVHASA"
     gene            6427..6600
                     /locus_tag="VGC69_01725"
     CDS             6427..6600
                     /locus_tag="VGC69_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294033.1"
                     /translation="MSTRPHNGWWKASNNASRRWLDQLKETPSADGKFAAPVVPASAV
                     PRLHVLEGRLQQS"
     gene            complement(6697..7032)
                     /locus_tag="VGC69_01730"
     CDS             complement(6697..7032)
                     /locus_tag="VGC69_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY0294034.1"
                     /translation="MKKCILAGAGAAALLLSSAALARIDVGIGVNLGAPVVVAPAPLV
                     VAPAPVYAPPAPVYVAPPAPVYMPPAPVYVAPRVVYPVPYYRGGPHYYHGPYRHPGHY
                     DPHRDPHRR"
     gene            7276..8541
                     /locus_tag="VGC69_01735"
     CDS             7276..8541
                     /locus_tag="VGC69_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0294035.1"
                     /translation="MEARSQRRKISAVCIALVVLVPLLLCTLMAMSEARRQVAGQAHL
                     VANLLQAQGESMARHEWNPDDRQDIVNLVNSVASLYACDIDASFSTGVSIHNDARSSE
                     VLHILDLAVTRQSQDKSVTVRVMMPGEKTLTLWRASLPAFLPVAVLLMALMGYSFWYM
                     DRQTPAHVLHRGMRRGEFYLKYQPIYDMARQCCAGAEVLMRWRPNGKAEVPPDVFIEQ
                     AEANDLIIPLTLHLFQLIERDLEKWPARPGFRLSVNIAAEHLSSPDFVGHIRAFRQRI
                     DRHGIILTVEITERSLVADDRQACANIETVRADGVRVAIDDFGVGHCSLSYLQRFNID
                     SLKVDKGFVNAIESAGDEAPILDAIIDLARRLNLPALVEGVETQAQRDYLAGKGVVFV
                     QGFLYSPPLPFDLLLAWLTGQEKMDKRDK"
     gene            8760..9716
                     /locus_tag="VGC69_01740"
     CDS             8760..9716
                     /locus_tag="VGC69_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0294036.1"
                     /translation="MASFDLAPLYRNCLLRSNEPLESHERVTQELSDHNLDWKSGRVD
                     TAMFQANTRQLSVFMLRYGAELEIRPKPFRHFTLMHFSLKGAAEFECDGGRLTVPEGR
                     TCIIAPRSDLRMRWQQGSEQLILKIPHSLLHDGGQGWTQLPPSSLLPQAHQAQWSLLM
                     QSLLQASGQQAGGTPARAAWLDHFERSVALFALAHHGGHTPPACPEPAREEGAGPRRL
                     DAMEAYMRSHLAAPVSLSDLAAAAGVSARTLGTLCRKHYGLTPMDLLRSWRLDAVRAR
                     LRVDASASVTATAFEFGFSHLGRFSAYYRERFGELPSQTVSH"
     gene            9853..11127
                     /gene="andAc"
                     /locus_tag="VGC69_01745"
     CDS             9853..11127
                     /gene="andAc"
                     /locus_tag="VGC69_01745"
                     /EC_number="1.14.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018618 - anthranilate 1,2-dioxygenase
                     (deaminating, decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate 1,2-dioxygenase large subunit
                     AndAc"
                     /protein_id="HEY0294037.1"
                     /translation="MSEQIVSASARPGRAVNFPQPDGAEVPYLVFSSQEVYDREQERI
                     YRGPTWNFLGLEAEIPNVHDFKSTFVGDTPVVVTRLAEGYACWVNRCGHRGAMLCRDK
                     RGNAASHSCVYHQWNFSASGDLQGVPFMRGQKGLKGSVGMPPDFKREDHGLQKLRVVN
                     YKGLLFATFSDETPPIEEYLGDDMRPWVDRIFHKPVVYLGCTRQYSKSNWKLYYENVK
                     DPYHASMLHLFHTTFNIFRVGMVARSLPDARYGLHSLITVTKPADDGGNSAAYAAQNI
                     RSMVEGFALEDPALLGQIQEYDEMTTNQIQTIFPQLVIQQIHNTLVARQILPKGPGNF
                     ELIFHFFGYADDTPELRELRLLQANLVGPAGYISMEDTEATELVQRATARDGDRHSYL
                     GLGRSTPDFTETVVTENLLRRFWVGYQKLMGY"
     gene            11132..11608
                     /gene="andAd"
                     /locus_tag="VGC69_01750"
     CDS             11132..11608
                     /gene="andAd"
                     /locus_tag="VGC69_01750"
                     /EC_number="1.14.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018618 - anthranilate 1,2-dioxygenase
                     (deaminating, decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate 1,2-dioxygenase small subunit
                     AndAd"
                     /protein_id="HEY0294038.1"
                     /translation="MDERMQLWVELSELQHRYVSALDNDQLEAWPGFFTEDGRYEIIP
                     KENFDAGLPAPIIYCRNRKMMRDRVVSLRNANIYEVHTYRHMVSGLIVQGSEGGVVKT
                     LSSYLVAITGQAGETSLYQAGAYLDEVVKVDGQWRYRSKRVVYDTLRVQTLLATPI"
     gene            11709..11921
                     /locus_tag="VGC69_01755"
     CDS             11709..11921
                     /locus_tag="VGC69_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294039.1"
                     /translation="MVFHMFIFPPWEFIIQVPWLPIHSRNDACGVFVAGDKTFQNISA
                     IHGSLLAGACEAEAVLVGLAARAGRK"
     gene            11991..13157
                     /locus_tag="VGC69_01760"
     CDS             11991..13157
                     /locus_tag="VGC69_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294040.1"
                     /translation="MSGSTKRVAFALFAGLCGLSAAHADPIKVGLALDISGPFAASGA
                     DARDGFDLAIEKLGGKLGGQPAEFIKTDYAGSPEQVTQLVNRYIQRDKISFFSGPIPS
                     NAALAAGPALFKARIPFISSNPGPSQYAGAQCSPYFFGQYQNDSYDEAAGKLANSKGY
                     KNVVIIAPDYPAGRDHMTGFKRTFTGALKGEIYTKVGQSDYAAEISQIRSDKPDAVYV
                     FLPGGMGINFIKQYVSSGLTAIPLVGNGSTADEDVIPAVGDAMLGMYNTAHWAHDLPI
                     PANTEFVAAFRKKYGGRYPSGYAAMAYDTIMAMDAAVHDAGGVGNPDALVKALANPTF
                     KSVRGDFTYGKNHYPIQDYYLRVVAKDADGKLTNKLVDTIMTKSQDAYVGECKL"
     gene            13170..14090
                     /locus_tag="VGC69_01765"
     CDS             13170..14090
                     /locus_tag="VGC69_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0294041.1"
                     /translation="MDAIFIFEQLLNGVGYGLMLFLIAAGLTLVFGIMDTMNLAHGSL
                     FMAGAYIAARVHQASGSFTGAVLIAIVATILISAVLELVIVRRLYGRDHLAQVLATFG
                     DILIADDAVQAIWGPSPIMAPTPAALAGPVQLVSGLPYPAYRLLLLGAGVLVALGMYL
                     LVNHTRIGMLVRAGASNRAMAEFMGVRVGRVFSFVFALGAALAALAGALMGPISAVQI
                     GMGEAVLIPALVVIVIGGIGSVRGAFVASLIVGLVDTSGRAFLPPLLRLLLPPAMASS
                     LGPALAGIAVYLLMAIILAVKPAGLFPARA"
     gene            14102..15109
                     /locus_tag="VGC69_01770"
     CDS             14102..15109
                     /locus_tag="VGC69_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0294042.1"
                     /translation="MTSSASLVSPSGAARAGIASRALPWALLVLLAAFPLVAPSLGLD
                     YYIDFVGKLLIVMIAATSLNFILGYGGMVALGHAGFMGVGAYTVAAMVAAGETSAWII
                     WPAALAVSAVFAAIIGAVSLRTRGVYFIMITLAFAEMLHYLAVSLRTYGGDDGYSIPT
                     PPSLGAWIDASPNSAYWAVLAIAALVFALVSRLAVSRFGYALMGVRDNETRMAALGFP
                     VFAIRLAAFTGSGALAGLAGALMACHNSFVSPSLMNWTESAILIIMVVIGGTGSRWGG
                     IIGAGLWLSLEEVLKSYTDYWHWPMGVLLIVIVFFAPKGIAALIEHAGGRQSGKQGGR
                     Q"
     gene            15106..15870
                     /locus_tag="VGC69_01775"
     CDS             15106..15870
                     /locus_tag="VGC69_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294043.1"
                     /translation="MSEALFHAEGLVKRFGGLVATNGVSIAVEKGEIHALIGPNGAGK
                     STLVNLVSGLLPLDEGRLTLDGVDLTALKPHQRVGAGLSRCFQVTSVFKADSVRKNLL
                     LAAQAHAGSSFRFLSRRAVETGLEETAFGLAQRVGLERDMDRVAGTLPHGAQRKLDVA
                     LALAARPKLLLLDEPMAGMGAEDSLRMTDLIQTLRADMAILLIEHDMDAVFRLADHIS
                     VLVYGKVLTSGNAQAIKSDPRVQEVYLGTETVEGAQ"
     gene            15867..16583
                     /locus_tag="VGC69_01780"
     CDS             15867..16583
                     /locus_tag="VGC69_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294044.1"
                     /translation="MSAPPLLSCKGVHAGYGASQVLFGIDLDIREKEVVTLLGRNGMG
                     KSTTIKTIIGALAARQGEISFGGKPLARMKPDAIARLGVAVVPEGRMCFPNLNVEEHL
                     VAFAANRNGQRDAWTLDRIYAVFPRLKERARHLGNQLSGGEQQMLAIGRALSTNPRLL
                     ILDEATEGLAPVIREEIWRCLATLRAAGQTILVVDKYVHRLVGLADRHIILERGKVVW
                     QGSSAELDADRGLWKAYLGV"
     gene            16683..17279
                     /locus_tag="VGC69_01785"
     CDS             16683..17279
                     /locus_tag="VGC69_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0294045.1"
                     /translation="MTAANSRSAEHPISDVFLKRWSPRAFTGEEISAETLATILEAAR
                     WAPSAYNFQPWRFIYARKGTAHWERLLGVLNPFNQSWAQSASALIIVLSKKAEIPPGK
                     DAEAPNRCHSFDAGAAWGYMALQAAMLGWQTHGMAGIEHDKIRAELGVPDGYEIEAGV
                     ALGRQGDKSTLPEGLQAREVPSGRRALGEITAEGKFAF"
     gene            complement(17287..17661)
                     /locus_tag="VGC69_01790"
     CDS             complement(17287..17661)
                     /locus_tag="VGC69_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294046.1"
                     /translation="MIATRRAPRYARLLGCAALLALVLSGCTQYQWYKDRATQDDYNQ
                     DTYACDMESAHAYPAVPAIGIVSPGFMTPPRTYCRARGCYTSPGYYIPPDMGTVDLNE
                     DNRSEAAKRCMYARGWQLIEVK"
     gene            complement(17670..19901)
                     /locus_tag="VGC69_01795"
     CDS             complement(17670..19901)
                     /locus_tag="VGC69_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEY0294047.1"
                     /translation="MELRIALLRRFLYSHYFFGGVRQAVGMLLPVMVLGGYFGEYRLG
                     LVATFGAQCLAIIDQPGGPQRHLPGEMLIGAGLGTVSVALTGLVSTHPVLLWLAVIAQ
                     CFFLSMFTVFGRRGSLIGFGALLLMTLTMHTPLSPDQVLMHAAASLGGALFYFCLSLL
                     SGRLLMLRAERQSLSAALFDTANYVAVRASFYDETTDLDEAYRQLILSQSAMTEKHQA
                     ARDNVLRALPTGRGRRNADKVMMWNMFVDMLQLRDTLVATHTDYILLRRALAGHDALI
                     FMRDALKKMALELDRIALDVARNQATQYRSSAKAELLAIEYEIERLRQREQDRPDPEV
                     ISLMVQVLRRMRNATRIVDRMADHTLMAPDAQPTSDLRIDKTLTRFMSRQRPNLGMIA
                     SNLRLDSPHCRYAIRVTLAAALGMLIIGSWSPSSDLSAHSYWVLLTIIIIMKPGFALT
                     RQRNFFRLVGTLIGCVLAPAMFLLTTKPPALFAMLMFACIMGNSLVQLNYMASSLFNT
                     LFVVLVFHFASPDTVSLEVIGERALDTVLGCALALACSYVLPWWESRSMQPLVLAATR
                     ANRDYLRAGLRYMRAMQAQRAGAADDKTAIAADAEDADLAWRLARKNVHNAFGNFAEA
                     FYRMMSEPRSHQSHVPELNHLLIQNHILASQITSVIPLLAGLAETPPALAQALEGMAR
                     QLDDTEPAPAGPPVQIETEGELAALAYPIKQMLRASDMIRDELAGLTDAPPRLPAQVA
                     TIG"
     gene            complement(19987..20775)
                     /locus_tag="VGC69_01800"
     CDS             complement(19987..20775)
                     /locus_tag="VGC69_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294048.1"
                     /translation="MQYRIDSKTENSLYFLEIVEEKSARITRHVLSVEITKSSQIQPS
                     SKRPVCYGHITIKVHYLSGPSQQAGTQICLMGGHVDSTSNMARLTNGNLVIENQALRG
                     LGLGSYLFNIIVTWILSFEVDFDIVPVRLFAGDAYPGNLERRNRFYEKFGLQFDYQTG
                     SGSMQTHGLSLPMKASGLVSLAMIPQGLYPHSLDWLAKEFKFILSLPLDNASSQGRNR
                     PPAFDEADPMPGPRFSLIEKLIYCLLLPAIFCLGFSLGKILSHF"
     gene            complement(20776..20967)
                     /locus_tag="VGC69_01805"
     CDS             complement(20776..20967)
                     /locus_tag="VGC69_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HEY0294049.1"
                     /translation="MPSIPYATLLAQILKETRKLQKQVEALKCKQRKTVLTSIIKSMR
                     EFGVSPEEIIEAYEKQKRY"
     gene            21261..22220
                     /locus_tag="VGC69_01810"
     CDS             21261..22220
                     /locus_tag="VGC69_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294050.1"
                     /translation="MSVELKLHQLECLVAVADHGSVRKAANYLGRSPTAVSNALRELE
                     LTVCSILVERKTEGAVVTDAGAALLAHARLILAQLQRAYDDAALLALQNGGMVRMAVT
                     PWLMQGVLPHVIKEFRAQRPDVQLNITEHFGIEYPAVRSGQLDLAFGPRPDSQHDHAL
                     EVTPLYSYSYAVVCRQGHPGESARTVSDLTGYDWLLSRPIEHIMPVVRDLIKAQTRKN
                     VCHFHYVPSLQATLAMIRSTDMLAIMPWPLLETPDLRHRFVALDMSPIMQESTTCLIT
                     RRYDPLQGPVLAFLDTFRRVARDLAASADPEVRRIFSMVESHM"
     gene            complement(22295..23524)
                     /locus_tag="VGC69_01815"
     CDS             complement(22295..23524)
                     /locus_tag="VGC69_01815"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HEY0294051.1"
                     /translation="MKIARVLFSTGISGYLHRDLAAIKAGARRNGVLYEGDPIAPGFR
                     RIVETADIVSVMLVLEDDQVAHGDCADVILAGFGGRDPAFRAADHQQALDLAIRPLLQ
                     GLDADDFRANAQRLDDCQPDGRPLHTAIRYGVSQALLHAAALSRKITITEVVAQDYGS
                     TIATEPVKLLASCQRDDAAQLDRMILKRVDLLPHANFMVPDDVGPQGEKLLDYARGIA
                     RRIQEIGEPDYHPRIHLDVYGTLGETFPDPAALLDYLERLAAAAAPYHLLLESPVIRA
                     SRSAQIDALRELRQALRSRGIDIGLIADEWCNTLEDAKLFADSQAADYLQIKTPDLGG
                     IHRSIEAVLYCRSRGMGACLGGTANETDQSARICAQVGLACGADFMLCKPGLGADEGL
                     MILKNEMMRTLALVRAR"
     gene            complement(23726..24703)
                     /locus_tag="VGC69_01820"
     CDS             complement(23726..24703)
                     /locus_tag="VGC69_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294052.1"
                     /translation="MSRLSRTLQRVALAALALCAPLAAARAAYPDHPVTWVVPYPPGG
                     TTDVVARYIAQALGKIMKASIVVENKGGAGGQIAMSYVARSAPDGYTLLVSDASLATA
                     PSLYRTMQVDPLKDLLPVALFVTVPHVLVVNPDVPARSLAELIALTKANPGKINFSSG
                     GIGSPLHLAGEALRLQTGLQWTHVPYKGAGPAIMAVVSGEAQTATPSLPAAVPQVQSG
                     KLRALAVTSPKRVAALPDVPSVVELGYPKAAVFGWVGLHAPVGTPADVLRAWEAAADQ
                     ALQDPELVQRLQAQGADLDLQSSAQYGKLVADEAARWKQVVNAAGIQPE"
     gene            complement(24700..25410)
                     /locus_tag="VGC69_01825"
     CDS             complement(24700..25410)
                     /locus_tag="VGC69_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294053.1"
                     /translation="MSALMGSGVLALWLDVSPELDRETDDWYVDEHLPERIDIGGYLR
                     ARRYRALRRAPGTPGYLSVFEATTPEALASEGYLSLVKRISPQSQRIRAGFSNVARNT
                     FRVRATAGRGLGAVLASYRLKANGPADAGALDAYALGLLRHRGVVGVHWLQAEPAVRQ
                     RMDAVRAVGQSDDLVDYALLVETTRETEADALRSTALADGPLAAQGWRAESIAVYGLL
                     YEVSSPLRDNETRLGDRT"
     gene            complement(25432..26415)
                     /locus_tag="VGC69_01830"
     CDS             complement(25432..26415)
                     /locus_tag="VGC69_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294054.1"
                     /translation="MRLFFLPRGRLAAWLAGLLLAPAAVLAQSYPSKPIHLVVPFPPG
                     GVADLIARPTAEKLGQLLGQPVIIENRGGATGTIGAAYVAHSAPDGYTLLFGTTNEIA
                     MSPTLYKSLPYDPTKAFAPVMPVAEFPNILVVGPTVSAASLADFVKQAKTQSRPLTFA
                     SSGTGSTNHLTAELFRSQADVSIVHIPYKGGGPALVDVAGGQVDALFATLPSAVSLIK
                     SGKLRALAVTGAKRTAALPDVPTAREAGMPDLVVTTWNGVLAPAGTPPAIVDTLHQAL
                     IQVVADPALKQTLALVGAETMTATPQEFAATIGKDYALWSGVIKHAGITAE"
     gene            complement(26476..27879)
                     /locus_tag="VGC69_01835"
     CDS             complement(26476..27879)
                     /locus_tag="VGC69_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0294055.1"
                     /translation="MTVKHETSVLEALARRVHEVRYDDLPGQVVDRAKALVLDTLGCA
                     YGGYGSEVGQAVLRMAASCGGAPQATLIGAGGKTSAPLATLVNGTLLRFLDSNDYYFA
                     RDPAHPSGNLAAALAVAEREHRSGKDLLAALAVAYEIHLRLCDFAGEPSLWKRGWHHG
                     TNAQFSSAALAARLAGDGPVRIAHAMAISGSHQNTLAQLQSGAVSMIKATAEAWVAKA
                     GVEAALLAGLGVTGPLPLMEGASGWAASVAGQVDFDALTAPMGERYRLLEVSIKPYPA
                     VATAVAPIRAAIALHRQGAPRVEDIERMIVKLPGFALGTPSAHPDRRYPSHIESAQHS
                     FYYCAAVALLDGACGDPQFEPGRLQAPDLRALLAKVELAEDPALNERWPAAAGGGVEL
                     RLRDGTVRRHDCPYPPGHPMMPLDAAELDAKFHGYADPVLTPPRARALREAVLNLEDC
                     PDIARLTGLFAAPEPAG"
     gene            complement(27895..>28626)
                     /locus_tag="VGC69_01840"
     CDS             complement(27895..>28626)
                     /locus_tag="VGC69_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0294056.1"
                     /translation="TALVTGASQGIGRATARMLAESGATVVGVARRKELVEQVAHGID
                     TTQGGRLIALAADFYDEDAPERVAAEAARLMGRVDILINAAGASRPVPFEAPREQWSE
                     GMVLNFFRIRELTHAVIPGMRENKWGRVVTFTGTSEPRMLNAAFTAKAAVHVWAKGLS
                     REVGASGITVNCIQPGRIHSEQMTKRYPTLESELEYARAEIPLGRFGEPEEIAGLAMF
                     LASEQAAYITGTVIPVDGGSSRFAF"
BASE COUNT         4722 a         9499 c         9544 g         4861 t
ORIGIN      
        1 ccggcggggc ctgatccgag gatcaggact ttcgcgtgtt tgggggtaga catgagatga
       61 gtatattgga gggtatgccc tcattataat gagcccatgc cgcgtatttc gaacgcctcc
      121 ccgcgcgcct cgcgcaatac ccgtaacggg ccgtcacctc tgcagtcgcg cctctccgcg
      181 ctgctgcgcg aggcccgttg gatcttcttt gccgcgttgg ccgtgtggct taccctggtg
      241 ctggccacct ggagcccatc cgaccccggt tggtcgcact cggtttccgg gggcgccctg
      301 cgcaacggcg gcggcgcggt cggcgcctac ctggccgaca tcctgctgta tttgttcggt
      361 ttttcggcct ggtggtgggt gatcctgctg gtgcatcggg tgcgggccgg ctaccgccgc
      421 ctggccacgc aattgcgcgc caccgggagc gagcccgaga ccctgccgcg cgtgcactgg
      481 gagcagggcg tgggcttcct gctgctgctg gccggttcgc tgggcctgga ggcgctgcgc
      541 ctgcgcagcc gcggcctgca cctgccgggc gccaccgagc atatcagcgg ggcgggcggc
      601 gtcatcggcc aactgctggc caactggctg tcgcgctccc tgggctttga cggcggcacg
      661 ctggcgctgc tggtgctggt ggcggtgggc ctgagcctgt tcctctcctt ctcgtggctg
      721 agcgtggccg agcgcatcgg cggctggctc gagcagggga tccgcggcat cgccaatgcc
      781 tacacggccc ggcaggaccg ccgcgtgggc gaggtggccc aggccgagcg cagcgagcag
      841 gtcgaggcca agcacgaaaa aatggtggtg cacgaaccca tccgcatcga acccgccatg
      901 accgtggtgc cgccctccga gcgcgccgcc aaggaaaagc agcagacgct cttcgagctg
      961 cccgagggcg aggccaacct gcccgagctc ggcctgctcg atccgcccct ggccagccag
     1021 gaaaccgtct cggccgagac catcgagttc acctcgcgtc tcatcgagaa aaaactggcc
     1081 gacttcggcg tgtcggtcgt cgtggtggcg gcccaggccg gcccggtcat cactcgttac
     1141 gaaatcgaac ccgccacggg cgtgaagggc agccagatcg tcaacctggc caaggacctc
     1201 gcccgcgccc tgagcctggt cagcatccgc gtggtcgaga ccatcccggg caagaacctc
     1261 atggggctgg agctgcccaa ccccaagcgc cagatggtgc gcctgtccga gatcctgggt
     1321 tcgcagacct accacgccgg ccagtcggtg ctgaccatgg ccctgggcaa ggacatcgcc
     1381 ggccatccgg tggtggccga cctggccagg atgccgcacc tgctggtggc cggcaccacc
     1441 ggctcgggca agtcggtggg gatcaacgcc atgatcctct cgctgctcta caaggccgac
     1501 gcctcgcaga cccgcctcat cctcatcgac cccaagatgc tcgaaatgag cgtctacgag
     1561 ggcatcccgc acctgctggc cccggtggtc accgacatgc gccaggccgc caacgcgctc
     1621 aactggtgcg tgggcgagat ggaaaagcgc taccgcctca tgagcaagat gggcgtgcgc
     1681 aacctggccg gctacaacgc caagatcaag gacgccatca agcgcgagga gcccatcccc
     1741 aatcccttct cgctcacgcc cgaccagccc gagcccctgc agtccatgcc catgatcgta
     1801 gtggtgatcg acgagctggc cgacctgatg atggtggtgg gcaagaagat cgaagaactc
     1861 atcgcccgcc tggcgcagaa ggcgcgcgcg gccggcatcc acctggtgct ggccacccag
     1921 cgccccagcg tggacgtcat caccggcctc atcaaggcca acatccccac gcgcatcgcc
     1981 ttccaggtct cgtccaagat cgactcgcgc accattctcg accagatggg cgccgaagcc
     2041 ctgctgggcc agggcgacat gctctacatg ccgcccggca ccggcctgcc cgtgcgggtg
     2101 cacggcgcct tcgtgcacga cgacgaggtc caccgcgtgg tcgaggcgct cagggcccag
     2161 ggcgagccca actacatcga aggcctgctc gagggcggca ccgagggcga ctcggccgat
     2221 ggcgccagca gcgtcaccgg catcggcggc gacgccgaat ccgatcccat gtacgaccag
     2281 gcctgcgagg tcgtgctcaa gaaccgccgc gcctcgatct cgctggtgca gcgccacttg
     2341 cgtatcggct acaaccgcgc cgcgcgcctg ctcgaacaga tggaaaacgc cggcctggtg
     2401 tcggccatgc agtccaacgg caaccgcgag attctggtgc cggcccggga ggaagcttga
     2461 tgcattgcgc agtgcgtgtg ttgggccgcg cggcagtcgc tgtcgcgatg gcctgcggcc
     2521 cgatgctggc ctggggcgcg acggcccagg aacagctcaa ggttttcgtg tcctcggtgc
     2581 agtcggccac gggttcgttc aaccaggcca cggccagcaa gcagggccgc aatccgccgg
     2641 cgcagcaagg cgcttttgcc ttccagcgcc cgggcaagtt ccgctggatg gtgcaaaagc
     2701 cgtacgagca gctcatcgtg tccgacggca agcaggtcta ccagtacgac cccgacctgg
     2761 cccaggtcac ggtgcgccag gtcgaccggg ccatcggcac gtcgccggcg gccatcctgt
     2821 tcggctcggg cgatctcgag caggccttca ccgtcacggc gctgcccgac cgcgacggcc
     2881 tgcaatggct gcgcgccaag ccgcgcaatg ccgacgcggg tttcgcgcag gtggacatcg
     2941 gcatgaaaga caacctgccg gcccgcatcc tgctgctcga cgccttcggc cagaccacgc
     3001 aggtggattt ttccggcctg cagcccaatg cggccgtgcc ggccacgcaa ttccagttca
     3061 cgccgcccca aggcgtggat gtcgtgaaga tgtaggtata ggcacgcatt gagatactcg
     3121 agccgaggac cccggagcca tccgaagcgc aggcgccttc tctgcccacg catcggaata
     3181 gcgcaggaca gcctcggcgg ctaaagcact caacaaatga gctccgacct tttcgccggc
     3241 gatcccgccc accgccccta cgtgccgctg gccgagcggc tgcgcccgcg cacgctggcc
     3301 gacgtggtgg gccagtcgca tctgctgggg ccggacaagc ccttgcgggt ggcgttcgcg
     3361 tcgggccggc cgcattccat gatcttctgg gggccgccgg gcgtgggcaa gaccacgctg
     3421 gcccggctca tggccgacgg cttcgacgcg caattcatcg ccatctcggc cgtcctgggc
     3481 ggggtgaagg acatccgcga cgcggtcacg gccgcgcaga tcgcgcaggg gcaggggcgc
     3541 cgcaccatcc tgttcgtcga cgaggtgcat cgcttcaaca aggcccagca ggatgccttc
     3601 ctgccctatg tggaaagcgg cctgttcacc ttcatcggcg ccaccaccga gaacccctcg
     3661 ttcgaggtca attccgcgct gctgtcgcgg gcgcgcgtct acgtgctgca ggcgctcacg
     3721 cccgaggagc tggcgctgct catcgaccgc gcggtcgacg ccatgaacga gggccgcggc
     3781 ggggaagcac agttgcccgt cctgcagatc acctccgagg cccgcgaaca actggccgcc
     3841 tgggccgacg gcgacgcgcg gcgcctgatc agcgccgtcg aagtcgtggc cgaatcggcc
     3901 ttgaccgccg aacgccatat cgtggatgcc gcctggctcg agaactcgct ctcgcagaac
     3961 ctgcggcgct tcgacaaggg cggcgaggcc ttctacgacc agatcagcgc gctgcacaag
     4021 tcggtgcgcg gctccgaccc ggatgcttcg ctgtactggt tctgccgcat gatcgacggc
     4081 ggcgccgatc ccctgtacct ggcccgccgc atcgtgcgca tggcctggga agacatcggc
     4141 ctggccgacc cgcgcgccgc gcaggtcgtc aacgacgccg cggccaccta cgaacggctg
     4201 ggctcccccg agggcgagct ggcgctgggc caggccgtca tctatctggc ctgcgccgcc
     4261 aagtccaacg cgggctacaa cgcctacaac gccgcgcgca aatacgccgc cgaacacggc
     4321 agcacgccgg tgcccctgca cctgcgcaat gccccgacca agctcatgaa agagctgggc
     4381 tacggccacg cctaccgcta cgcccacgac gagccgcacg gctacgcggc gggcgagcag
     4441 tatttccccg acggactgga aaccaccttc taccgcccga ccgatcgcgg gctagaggct
     4501 aaaatccagc agaagctggc tttcctgaaa gacctggacc gccagcacaa gaaaaagtcg
     4561 tgaccggcca gccatacgtc cggctggcgc aaacccgagc cgtggacccc ggaggaatcc
     4621 gaagcacagg cgctacctct gcctacgcat cggaattccg cagggcagcc acggcggctt
     4681 aagaacccag atagacgtgt aggccaataa ccaaatgcta gaccccaccc tgctgcgcaa
     4741 agacctgcaa accgtcgtcg atcggctcaa gacccgcaaa gtcgacttcg acaccgaggc
     4801 cttcaacgcc ctcgaatccc gccgcaagac cgtgcagacc gaaaccgaga ccctgcaggc
     4861 gcgccgcaac gcgctggcca agcagatcgg ccagctcaag gccaagggcg aggacgcctc
     4921 aagcgtcatg gccgagtccc agggcattcc ggcccgcctc aagcagctcg aggacgatct
     4981 ggccgcggtg caggcgcaac tgaacgactt gctcatgtcg gtgcccaacc tgccgcacgc
     5041 cagcgtgccg gtgggcaagg acagcgacgg gaacgtcgaa gtgcgccgct ggatcccggg
     5101 cgatcccaag gtcaacgcgg gccccgacgg caatccggcc ggcctgggtt tcgagccgcg
     5161 cgaccacgtc gccataggcg agcccctggg cctggatttc gacctggccg cgcggttgtc
     5221 gggcgcgcgc ttctcgttca tgcgcggtcc cgtcgcccgc ctgcaccgcg ccctggcgca
     5281 gttcatgctc gacctgcaga cgggcgagca cggctacacc gagtgctaca ccccctacat
     5341 cgtcaacagc tccaccttgt tcggcaccgg ccaactgccc aagttcaagg acgatatgtt
     5401 ctttgtgacc aaaggcggcg gcgacgacga tcccaaggtc gacgagcagg gcaggcccct
     5461 ggtgcgcgaa gaccagtacc tgatctccac ttccgagatc accctgacca gcgccgtgcg
     5521 cgacaccatc gtgccggcgg gcggcctgcc catgcgcctg acggcgcata ccccgtgctt
     5581 tcgttccgag gcgggcagcg gcgggcgcga cacgcgcggc atgatccgcc agcaccagtt
     5641 cgacaaggtc gagatggtgc agatcgtcga gcccgacacg tcctacgaca cgctcgagca
     5701 gatggtcggc cacgccgagc aggtgctgcg ccgcctgggc ctgccctacc gggtgatgct
     5761 gctgtgcacg ggcgacatgg gtttcggcgc ggccaagacc tacgacctcg aggtctggct
     5821 gccggcgcag aacacctggc gcgagatctc gtcggtgtcc aactgcgagg cgttccaggc
     5881 ccgccgcatg caggcgcgtt tccgcaacgc ccagaacaag ccggaattcg tccataccct
     5941 caacggctcc ggcctggccg tgggccgtgc cctggtggcg gtgctcgaga actaccagca
     6001 ggccgacggc agcgtggtcg tgcccgaggc cctgcgcccc tacatgggcg gcgccgaggt
     6061 gctcaagccc taggcgccag gccgacagcg cttgcatttt ttgtcacaaa agcccgaaat
     6121 cccttgccgg ttccgggctt tttcgacata tctctgactt gtgttgtggt tatcctgccg
     6181 tggccattga ggaagtagcc atgacaatgc gaaccatcgg tgatttccgg gtgcgatgca
     6241 cgatcgtgca agaaaacccc cgccaattca gcgtgcaagt gtgcacgcgc aaggtgggag
     6301 gcaacgctcc cgaaaaatgc tgggatgtcc cgggtctggc cttcgatagc cgcgccgcgg
     6361 ccgagcaggg gagcctgcag gtgttcgagc gcatcaatgg cgtacgtttc aacggggagc
     6421 ccgaatttgt ccacgcgtcc gcataacggc tggtggaaag cgtccaacaa cgcttcgcgc
     6481 cgctggcttg atcaactgaa agaaacgccg tccgccgacg gcaagtttgc cgcgccggtc
     6541 gttccggcgt cggctgtgcc gcgcctgcac gtcctggaag gccgcctgca gcaaagctga
     6601 cccgatgtcc ggcgcccatg tccggggcgg tttcccgggc ataaaaaacg ccgcccaagg
     6661 cttgcgcccc gggcggcgtt tttctttagc gggaaatcag cggcgatgcg gatcgcggtg
     6721 cgggtcgtag tgcccgggat gacggtaggg gccatggtag tagtgcgggc cgccacggta
     6781 gtaggggacc gggtagacca cgcgcggcgc gacgtaaacc ggcgccggcg gcatgtaaac
     6841 gggtgccggc ggggccacgt agaccggcgc cggcggcgca tagaccgggg ctggcgcgac
     6901 gaccagcgga gcgggggcaa ccacgacggg ggcgccgaga ttgacgccga tgcccacgtc
     6961 gatccgggcc agggccgcgc tggaaagcag cagggctgcg gcgccagcgc ccgccaagat
     7021 gcacttcttc atttttccac ctgccatact gccaaaggca gcaaagttgc gaatatttca
     7081 gccaatattg tctcgcacgc gcggcgtgca atgcacgtat tcccgggcat ggaatcgcga
     7141 caatctgttt atcagcgtgt gtatttgcaa agcttgaaac ttgtcccggg cggaaaagtt
     7201 tccgcggcat cgaggtttcc atggcatgca tggcgggatc gtttgcggct tccctgctac
     7261 gatcctgcca tgggcatgga ggcacggagt caaagaagaa aaatcagcgc ggtgtgcatc
     7321 gcgctggtcg tgctcgttcc tttgctgctc tgcaccctga tggccatgtc cgaggcgcgc
     7381 cgccaagtgg cggggcaggc ccatctcgtg gcgaatctgc tgcaggcgca gggcgagtcc
     7441 atggcccgtc acgagtggaa cccggacgac aggcaggata tcgtcaacct ggtcaattcc
     7501 gtcgcctccc tgtacgcctg cgatatcgac gcgagcttca gcaccggggt gtccatccac
     7561 aacgatgcgc gttcgagcga agtcctgcac atcctggacc tggccgtcac ccggcagtcg
     7621 caggacaagt cggtcacggt cagggtgatg atgccgggtg aaaaaacgct gaccctctgg
     7681 cgggcgtcgt tgccggcatt cctgcccgtg gccgtgctgc tgatggcgct gatgggctat
     7741 tcgttctggt atatggacag gcagacgccg gcccacgtac tgcaccgggg aatgcgccgc
     7801 ggcgagttct acctgaagta ccagcccatc tacgacatgg cccgccaatg ctgcgccggg
     7861 gccgaagtcc tgatgcgctg gcgcccgaat ggcaaagccg aggtgccgcc cgacgtgttc
     7921 atcgaacagg cggaggccaa cgacctgatc atcccgctga ccttgcatct tttccagctc
     7981 atcgagcgcg acctggaaaa gtggcctgcg cggcccgggt tccgcctgag cgtcaacatt
     8041 gcggccgagc atctttccag tcccgatttc gtcgggcata tccgcgcctt ccggcagcgc
     8101 atcgacaggc acggcatcat cctgacggtc gagatcacgg agcgcagcct ggtggccgac
     8161 gacaggcagg cctgcgccaa tatcgagacc gtgcgcgccg acggcgtgcg ggtggccatc
     8221 gacgatttcg gcgtgggcca ctgctcgctg tcgtatctgc agcgcttcaa catcgactcg
     8281 ctgaaggtcg acaagggctt cgtcaatgcc atcgaatcgg ccggcgacga ggcgcccatc
     8341 ctggacgcca tcatcgacct ggcccggcgg ctgaatctgc cggccctcgt cgagggggtc
     8401 gagacgcagg cccagcgcga ctacctggcc ggcaagggag tggttttcgt gcaaggattc
     8461 ctgtacagcc ctcccttgcc tttcgacctg ctgctcgcct ggctgacggg acaggaaaaa
     8521 atggataaaa gggacaaata agtcccggtt gcacgcccgc catcgtccgt gcttcatcct
     8581 ggtgcgtgat gcgtgtccgg gcgtggtgcc ggcgcatcgc ggccatggcc tgacgtgccg
     8641 atgccggcgc ccggccgggg gcgtatcggg taattcccgt ctggcacaag tcttgctaat
     8701 tccttaaaag agtttaaaag accgtgacat cttctccgca tccgccttga gacacccccg
     8761 tggcctcgtt cgacctcgcg ccgctctatc gaaactgcct cttgcgctcc aatgagccgc
     8821 tggagtcgca cgagcgcgtc acgcaggagt tgtccgatca caatctcgac tggaagtccg
     8881 gccgggtcga caccgccatg ttccaggcca atacccggca gctcagcgtg ttcatgctgc
     8941 gctacggggc cgagctggaa atccgcccga agccctttcg ccatttcacc ctgatgcact
     9001 tctcgctcaa gggcgcggct gaattcgaat gcgacggcgg ccgcctgacc gtgcccgagg
     9061 ggcgcacctg catcatcgcg ccgcgcagcg atctgcggat gcgctggcag caggggtccg
     9121 agcagctcat cctcaagatc ccccacagcc tgctgcacga cggcgggcag ggctggacgc
     9181 agctgccgcc ttcctccctg ctgccgcagg cccaccaggc ccagtggtcg ctgctgatgc
     9241 agtcgctgct gcaggcctcc gggcagcagg ccggcgggac gccggcccgt gccgcgtggc
     9301 tcgatcattt cgaacgttcc gtggccctgt tcgccctggc gcaccatggc ggccataccc
     9361 ccccggcctg ccccgagccg gcccgggaag agggcgccgg cccgcggcgg ctcgacgcca
     9421 tggaagccta tatgcgcagc cacctggccg cgccggtgtc cttgtccgac ctggccgccg
     9481 ccgccggggt cagcgcgcgc acgctgggca cgctatgccg gaagcactac ggcctgacgc
     9541 ccatggacct gctgcgcagc tggcgcctgg acgccgtcag ggcacgcctg cgggtcgacg
     9601 cttcggccag cgtcacggcc acggccttcg aattcgggtt cagccacctg ggccgcttct
     9661 cggcctacta tcgcgaacgc ttcggcgaac tgcccagcca gaccgtcagc cactgatccc
     9721 gtccggcgtc ggggatttcc ctggcttttc cagattcgga caaagtctgc gccgcgactg
     9781 ctttttccgg atagcaaggg gcacggcggc tttctagact ggcggcaacc gtttttcagg
     9841 aaggaacccg ccatgtctga gcaaatcgtc agtgcatcgg cccgcccggg ccgcgcggtc
     9901 aattttccgc agcccgacgg cgccgaggtg ccctacctcg tcttcagttc gcaagaggtc
     9961 tacgaccgcg agcaggagcg catctaccgc ggccccacct ggaacttcct gggcctggaa
    10021 gccgagatcc ccaacgtcca cgacttcaag agcaccttcg tgggcgatac cccggtggtg
    10081 gtcacccgcc tggccgaagg ctacgcctgc tgggtcaacc gctgcgggca ccgcggcgcc
    10141 atgctgtgcc gcgacaagcg cggcaatgcc gcgtcgcaca gctgcgtcta tcaccagtgg
    10201 aacttctcgg ccagcggcga cctgcagggc gtgcccttca tgcgcggcca gaagggcctg
    10261 aaagggtcgg tgggcatgcc gcccgacttc aagcgcgaag atcacggcct gcagaagctg
    10321 cgggtcgtca actacaaggg cctgctgttc gccaccttca gcgacgagac cccgccgatc
    10381 gaagaatacc tgggcgatga catgcgcccg tgggtcgacc gcatcttcca caagcccgtc
    10441 gtctacctgg gctgcacgcg ccagtactcc aagtcgaact ggaagctcta ctacgagaac
    10501 gtgaaggacc cctatcacgc gagcatgctg cacctgttcc acaccacgtt caacatcttc
    10561 cgcgtgggca tggtggcccg gtcgctgccc gacgccaggt acggcctgca cagcctcatc
    10621 accgtgacca agccggccga cgacggcggc aactccgccg cctatgccgc gcagaatatc
    10681 cgttcgatgg tcgagggatt cgccctggag gatcccgcgc tgctgggcca gatccaggaa
    10741 tacgacgaga tgaccaccaa ccagatccag acgatctttc cgcaactggt catccagcag
    10801 atccacaaca ccctggtggc gcggcagatc ctgcccaagg ggcccggcaa cttcgaactg
    10861 atcttccatt tcttcggcta cgcggacgac acgcccgagc tgcgcgagct gcgcctgctg
    10921 caggccaacc tggtggggcc ggccggctat atctcgatgg aagacaccga agccacggaa
    10981 ttggtgcagc gcgccacggc gcgcgacggc gaccggcact cctacctggg cctgggccgc
    11041 agcacgcccg acttcaccga aaccgtggtg accgaaaatc tgctccgccg cttctgggtg
    11101 ggctaccaga agctgatggg gtattgatca catggatgaa cgcatgcaac tctgggtgga
    11161 actgagcgaa ctgcagcatc gctacgtcag cgcgctcgac aacgaccagc tcgaagcctg
    11221 gcccggcttc ttcaccgaag acggccgcta cgagatcatc cccaaggaaa acttcgacgc
    11281 cggcctgccc gcgcccatca tctactgccg caaccggaag atgatgcgcg accgcgtggt
    11341 gtcgctgcgc aacgccaata tctatgaagt ccatacttac cgccacatgg tgtcgggcct
    11401 gatcgtccag ggcagcgagg gcggcgtggt caagacgctg tcgagctacc tggtggcgat
    11461 caccggccag gcgggcgaga ccagcctcta tcaggccggc gcctatctcg acgaagtcgt
    11521 caaggtcgat gggcagtggc gctaccggag caagcgcgtg gtgtacgaca ccctgcgcgt
    11581 gcagaccctg ctggccacgc ccatctgacc gattccatgc cccatgttcc ggagatcgga
    11641 ttgatcgccg ccggcaaggc cgagcgggcg tcgggcctgg gcgatcgggc tgccgtgaac
    11701 ggctcatcat ggtattccac atgtttatat ttccgccctg ggaattcatt attcaagttc
    11761 cttggctacc gatacattcc cgcaacgatg cctgcggcgt attcgtcgca ggcgataaaa
    11821 ctttccagaa tatcagcgcc atccacggca gcctgctggc cggggcgtgc gaagccgagg
    11881 ccgtcctggt cggcctcgcc gcgcgcgccg gccgcaaata acggaatgcc gtgctttaac
    11941 ccagcgcggc gttccggtct ggtttcatgt cgcacaacaa ggagagttac atgagcggtt
    12001 ccaccaagcg cgttgcgttt gccctgtttg ccggcctgtg cggcttgtcg gctgcccacg
    12061 cggacccgat caaggtcggc ctggccctgg atatctcagg ccccttcgcg gcctccggcg
    12121 ccgacgcgcg cgacggcttc gacctggcca tcgaaaagct gggcggcaag ctcggcggcc
    12181 agccggccga attcatcaag accgactacg ccggcagccc ggaacaggtc acccaactgg
    12241 tcaaccgcta catccagcgc gacaagatct cgttcttctc gggtcccatt ccgtcgaacg
    12301 cggcgctggc ggccggcccc gcgctgttca aggccaggat ccccttcatc tcgagcaacc
    12361 ccggccccag ccagtacgcg ggcgcccagt gcagccccta tttcttcggc caataccaga
    12421 acgacagcta cgacgaggcc gcgggcaagc tggccaacag caagggctac aagaatgtcg
    12481 tgatcatcgc cccggactat cccgcgggcc gcgatcacat gaccggcttc aagcgcacgt
    12541 tcaccggcgc gctcaagggc gagatctaca ccaaggtcgg ccagagcgac tacgccgccg
    12601 agatctcgca gatacgcagc gacaagcccg acgcggtcta cgtcttcctg ccgggcggca
    12661 tgggcatcaa cttcatcaag cagtatgtgt ccagcggcct gaccgccatt cccctggtgg
    12721 gcaacggctc cacggccgac gaggacgtga tccccgccgt gggcgacgcc atgctgggca
    12781 tgtacaacac ggcgcattgg gcgcacgacc tgccgatccc ggccaatacc gagttcgtcg
    12841 cggccttccg caagaagtac ggcggccgct atccctcggg ctacgccgcc atggcctacg
    12901 atacgatcat ggccatggac gccgccgtgc acgatgcggg cggcgtcggc aacccggatg
    12961 ccctggtcaa ggccctggcc aaccccacct tcaaatcggt gcgcggcgac ttcacctacg
    13021 gcaagaacca ctatcccatc caggactact atctgcgcgt ggtggccaag gatgccgacg
    13081 gcaagctcac caacaaactg gtggacacca tcatgaccaa atcccaggat gcctatgtcg
    13141 gcgaatgcaa gctgtaaatc gacattctga tggacgccat attcatcttc gagcaactgc
    13201 tcaacggcgt cgggtacggc ctcatgctgt tcctgatcgc cgccgggctc acgctggtat
    13261 tcggcatcat ggacaccatg aacctggccc acgggtccct gttcatggcg ggcgcctata
    13321 tcgccgcgcg cgtgcaccag gccagcggct ccttcaccgg cgccgtgctg atcgccatcg
    13381 tggccacgat cctgatctcc gccgtgctgg agctcgtcat cgtgcgccgg ctgtacggcc
    13441 gcgatcacct ggcccaggtg ctggccacct tcggggacat ccttatcgcc gatgacgcgg
    13501 tccaggccat ctgggggccg tcgcccatca tggcgcccac gccggccgcg ctggccggcc
    13561 cggtgcaact ggtgagcggc ctgccgtatc ccgcctaccg cctgctgctg ctgggcgcag
    13621 gcgtcctggt ggcgctgggc atgtacctgc tggtcaacca cacgcgcatc ggcatgctgg
    13681 tgcgcgccgg ggcgtccaac cgcgccatgg ccgaattcat gggcgtgcgc gtgggccgcg
    13741 tgttttcctt cgtgttcgcg ctgggcgccg cgctggccgc gctggccggg gccctgatgg
    13801 ggccgatcag cgcggtgcag atcggcatgg gcgaggccgt cctcatcccg gcgctggtgg
    13861 tcatcgtcat cggcggcatc ggttcggtgc gcggcgcatt cgtggcctcc ctcatcgtcg
    13921 gcctggtgga cacctcgggc cgcgccttcc tgccgccgct cttgcggctg ctgctgccgc
    13981 cggccatggc gtccagcctg ggcccggcgc tggccggcat cgcggtgtac ctgctcatgg
    14041 cgatcatcct cgccgtcaaa cccgcgggcc tgttccccgc gcgcgcctga aggccttgtc
    14101 catgacttct tccgcgagcc tcgtttcccc gtccggcgcc gcgcgcgccg gcatcgccag
    14161 ccgcgccttg ccctgggcgc tgctggtgct gttggcggct ttccccctgg tggcgccgag
    14221 cctcgggctg gactactaca tcgatttcgt cggcaagctg ctgatcgtca tgatcgccgc
    14281 caccagcctg aacttcatcc tgggctacgg cggcatggtg gccctggggc atgccggctt
    14341 catgggggtg ggggcctata ccgtggcggc catggtcgcg gcgggcgaga cgtccgcctg
    14401 gatcatctgg ccggcggcgc tggccgtcag cgccgtgttc gccgcgatca tcggcgcggt
    14461 gtcgctgcgc acgcgcggcg tctacttcat catgatcacg ctggcctttg ccgagatgct
    14521 gcattacctg gcggtgtcgc tgcgcacgta cggcggcgac gacggctaca gcatcccgac
    14581 cccgccgtcg ctgggcgcct ggatcgatgc ctcgcccaac agcgcctatt gggcggtgct
    14641 ggccatcgcc gcgctggtct tcgccctggt gagccggctg gccgtttccc gtttcggcta
    14701 tgccctgatg ggcgtgcgcg acaacgaaac gcgcatggcg gccctgggct ttccggtgtt
    14761 cgccatccgg ctcgcggcct ttaccggttc cggcgcgctg gccggcctgg ccggcgccct
    14821 gatggcctgc cacaacagct tcgtgagccc gtcgctgatg aactggaccg agtcggccat
    14881 cctcatcatc atggtggtga tcggcggcac gggcagccgc tggggcggca tcatcggcgc
    14941 cggcctttgg ctgtcgctcg aggaagtgct caagagctat accgactact ggcactggcc
    15001 catgggggtg ctgctgatcg tcatcgtgtt cttcgcgccc aagggcatcg ccgcgctgat
    15061 cgagcacgcg ggcggcaggc aaagcggcaa acaggggggc aggcaatgag cgaggcactc
    15121 tttcatgccg agggcctggt caagcgcttc ggcggcctgg tcgccaccaa cggcgtgtcc
    15181 atcgccgtcg aaaagggcga gatccacgcg ctcatcggcc ccaacggcgc cggcaaatcg
    15241 acgctggtca acctggtctc cggcctgctg ccgctggacg aaggcaggct cacgctcgac
    15301 ggcgtcgacc tcaccgcgct caagccgcac cagcgcgtgg gcgcgggcct gtcccgctgc
    15361 ttccaggtca ccagcgtgtt caaggccgac agcgtgcgca agaacctgct gctggccgcg
    15421 caggcgcatg ccggctccag cttccgcttc ctgtcccggc gtgcggtcga gaccgggctg
    15481 gaagaaaccg ccttcggcct ggcgcagcgc gtcggcctgg agcgcgacat ggaccgcgtc
    15541 gccggcacgc tgccgcatgg cgcgcagcgc aagctcgacg tggcgctggc cttggccgcg
    15601 cgtcccaagc tgctgctgct cgacgaaccc atggccggca tgggggccga agactccctg
    15661 cggatgaccg atctcatcca gactttgcgc gccgacatgg ccatcctgct catcgagcac
    15721 gatatggatg ccgtgttcag gctggccgac catatctcgg tgctggtcta cggcaaggtg
    15781 ctgacctccg gcaatgcgca ggccatcaag agcgatccac gcgtgcaaga ggtctacctg
    15841 ggtaccgaaa ccgtggaggg agcccaatga gcgcgccgcc gctattgtcc tgcaaggggg
    15901 tgcatgccgg ctatggcgcc agccaggtgc tgttcggcat cgacctcgac atccgcgaaa
    15961 aagaagtggt gacgctcctg ggccgcaacg gcatgggcaa gagcaccacc atcaagacca
    16021 tcatcggcgc gctggccgcg cgccagggcg agatcagctt cggtggcaag ccgctggcgc
    16081 ggatgaagcc cgacgccatc gcccgcctgg gcgtggcggt ggtgcccgaa ggccgcatgt
    16141 gctttcccaa cctgaacgtc gaggaacacc tggtggcctt cgccgccaac cgcaacggcc
    16201 agcgcgatgc ctggacgctg gatcgcatct acgccgtctt cccccggctg aaagagcgcg
    16261 cccgccatct cggcaaccag ctctccggcg gcgaacagca gatgctggcc atcggcaggg
    16321 cgctgtcgac caatccgcgc ctcctgatcc tggacgaggc caccgaaggc ctggcgcccg
    16381 tcatccgcga ggaaatctgg cgctgcctgg ccacgctacg cgcggctggg cagaccatcc
    16441 tggtcgtgga caaatacgtg catcgcctgg tcggcctggc cgaccggcac atcatcctcg
    16501 aacgcggcaa ggtcgtgtgg caaggcagct cggccgagct cgacgccgac cgcggcctct
    16561 ggaaggcgta tctgggggtg taaccgcgac gtggccgcgg acatgcgccg ttcgccgcat
    16621 ggggcacgat acactgatgc ggcttctgca agcgcttttg caaccgatct caaaggacca
    16681 tcatgaccgc agccaattcc cgcagtgccg agcacccgat ttccgacgtt ttcctcaagc
    16741 gctggtcgcc ccgtgccttc acgggcgagg aaatctctgc cgagacgctg gccaccatcc
    16801 tggaagccgc ccgctgggcg ccgtcggcct acaacttcca gccctggcgc ttcatctacg
    16861 cgcgcaaggg cacggcgcac tgggagcgcc tgctgggcgt gctcaacccc ttcaaccaga
    16921 gctgggccca atcggcttcg gccctgatca tcgtcctgtc caagaaggct gaaatcccgc
    16981 ccggcaagga cgccgaagcg ccgaaccgct gccacagctt cgacgccggc gcggcctggg
    17041 gctacatggc cctgcaggcc gccatgctgg gctggcagac gcacggcatg gccggcatcg
    17101 agcacgacaa gatccgcgcc gagctcggcg ttccggatgg ctatgaaatc gaagccggcg
    17161 tggcgctcgg ccgccagggc gacaagtcca cgctgcccga agggctgcag gcccgcgagg
    17221 ttcccagcgg ccgccgcgcg ctcggagaaa tcacggccga aggcaagttc gccttctgat
    17281 cccggcttac ttcacctcga tcaactgcca gccgcgcgca tacatacagc gcttggctgc
    17341 ctcgctgcgg ttgtcctcgt tgaggtcgac ggtgcccatg tccggcggga tgtagtagcc
    17401 cggcgaggta tagcagcccc tcgcccggca gtacgtgcgg ggcggcgtca tgaagccggg
    17461 cgatacgatc ccgatggcgg gcacggccgg ataggcgtgg gccgattcca tatcgcaggc
    17521 gtaggtatcc tggttgtagt cgtcctgcgt ggcgcggtcc ttgtaccact ggtattgcgt
    17581 gcatccggac aggaccagcg ccagcagtgc ggcacagccc agcaggcggg cgtagcgggg
    17641 cgcgcgtcgg gtagcaatca tttccggcct tatcctatcg ttgcgacctg cgccggcagc
    17701 ctgggcggcg catcggtcag gccggccagt tcatcgcgga tcatgtcgct ggcgcgcagc
    17761 atttgcttga tggggtaggc cagcgccgcc agctcgccct cggtctcgat ctgcacgggc
    17821 gggcccgcgg gggcaggttc ggtgtcgtcg agctggcgcg ccatgccttc cagggcctgt
    17881 gccagtgccg gcggtgtttc ggccaggccg gccaatagcg ggatcaccga ggtgatttgc
    17941 gaggccagga tgtggttctg gatcagcaga tggttcagct ccggcacgtg cgactggtgc
    18001 gagcgcggct cgctcatcat gcggtagaag gcttcggcga agttgccgaa ggcattgtgc
    18061 acgttcttgc gggccaggcg ccaggccagg tcggcatcct ccgcatcggc cgcgatggct
    18121 gtcttgtcat ccgccgcgcc ggcgcgctgg gcctgcatgg cgcgcatata gcgcaagccc
    18181 gcccgcaagt agtcgcggtt ggcccgggtg gcggccagca ccagcggctg catcgaacgc
    18241 gattcccacc agggcagcac gtagctgcag gccagcgcca gcgcgcagcc cagcacggtg
    18301 tccagggcgc gttcgccgat gacctccagc gacacggtgt cgggcgaggc gaagtgaaat
    18361 accagcacca cgaacaaggt gttgaacagc gagctggcca tgtagttcag ctgcaccagg
    18421 ctgttgccca tgatgcaggc gaacatcagc atggcgaaga gtgccggtgg cttggtggtg
    18481 agcaggaaca tcgccggagc cagcacgcag ccgatcagcg tgcccaccag ccggaagaaa
    18541 ttgcgctggc gcgtcagcgc gaagcccggc ttcatgatga tgatgatggt gagcagcacc
    18601 cagtagctgt gggccgacag gtcgctggac ggcgaccagc tgccgatgat gagcatgccc
    18661 agcgcggcgg ccagggtgac gcgaatggcg tagcggcagt gcggcgaatc cagccgcagg
    18721 ttgctggcga tcatgcccag gttcggtcgc tggcgcgaca tgaagcgtgt cagcgtcttg
    18781 tcgatgcgca ggtcgctggt gggctgcgcg tcgggggcca tcagggtgtg gtcggccatg
    18841 cggtcgacga tgcgggtggc gttgcgcatg cggcgcagca cctgcaccat caggctgatg
    18901 acttcggggt ccggccggtc ttgttcgcgt tggcgcagcc gctcgatctc gtattcgatg
    18961 gcgagcagct cggccttggc gctgctgcgg tactgggttg cctggttgcg cgcgacatcc
    19021 agggcgatgc ggtctagctc cagcgccatt ttcttcagtg cgtcgcgcat gaagatcagc
    19081 gcgtcgtggc cggccagggc gcggcgcagc aggatgtagt cggtatgcgt cgccaccagg
    19141 gtatcccgca gctgcagcat gtcgacgaac atattccaca tcatgacctt gtcggcgttg
    19201 cggcggccgc ggccagtggg cagcgcgcgc agcacgttgt cgcgcgcggc ctggtgcttc
    19261 tcggtcatgg ccgactggct gaggatcaac tgccggtagg cctcgtccag gtcggtggtt
    19321 tcgtcgtaga aactggcgcg cacggccacg tagttggcgg tgtcgaacag cgccgccgac
    19381 aggctctgcc gttccgcgcg cagcatcagc aggcggccgg agagcaggct caagcagaaa
    19441 taaaagagcg ctccgcccag tgaggcggcg gcatgcatca atacctggtc gggcgacagc
    19501 ggggtgtgca tggtgagcgt catcagcagc agcgcgccaa agccgatcag gctgccgcgc
    19561 cggccgaaca ccgtgaacat cgacaggaag aaacactgcg cgatgaccgc aagccacagc
    19621 agaaccgggt gggtcgagac cagcccggtc agcgcgaccg acaccgtgcc gagcccggcg
    19681 ccgatcagca tctcgccggg caggtggcgc tgcgggccgc ctggctggtc gataatggcc
    19741 aggcattgcg cgccgaaggt ggcgaccaga ccgagccggt attcaccgaa ataaccgccc
    19801 agcaccatga cgggcagcag catgcccacg gcctgccgca ctccgccgaa gaagtaatgg
    19861 ctgtagagaa aacgccgtaa gagagcgatg cgcaattcca tgcttgtcca tcctgaatgg
    19921 cgtcagtata gacaggctcg gatcatccca gctgcgccgc ttgcctcgat ttcggtttgg
    19981 cggccgtcaa aaatggctca ggattttccc cagggaaaaa cccaggcaga aaatcgccgg
    20041 gagcagcagg cagtaaatca gtttctcgat cagggaaaac cgcgggcccg gcatcgggtc
    20101 cgcctcgtcg aaagcaggcg gtcgattccg accctgactc gatgcgttat ccagcggcag
    20161 cgacaaaata aatttgaatt ccttggccag ccagtccagg gagtggggat ataagccctg
    20221 ggggatcatg gccagcgaaa caagcccgct ggccttcatg ggcagggata gtccgtgcgt
    20281 ctgcatggaa ccgcttccgg tctggtagtc gaattgcaat ccgaattttt cgtagaaccg
    20341 gtttctccgt tcgagattgc cgggataggc atcgcccgcg aaaagccgga caggcacgat
    20401 atcgaaatcc acctcgaaac tcaggatcca cgtcacgata atgttgaaga gataagagcc
    20461 caggcccagg ccgcgcaagg cctggttttc tatgaccagg ttgccgttgg tcagccgtgc
    20521 catattgctg gtcgaatcca catgtccgcc catcaggcag atttgcgtgc cggcttgctg
    20581 gctcgggccg gacaaataat gaacctttat cgtgatgtgt ccgtagcaga cgggacgttt
    20641 cgagctgggc tggatctggc tggatttcgt gatttcgacg ctcaggacat gccgtgtgat
    20701 ccgagcgctt ttttcttcga cgatctcgag aaaatacagc gagttttcgg tcttggagtc
    20761 gatgcggtac tgcattcaat accttttctg tttttcgtac gcctcgatga tctcttcggg
    20821 cgaaaccccg aattcacgca ttgacttgat gatggaagtc aggacggttt tccgttgttt
    20881 gcatttcagg gcttcgacct gtttttgcag ctttcttgtt tctttcagga tctgtgctag
    20941 cagggtggca tacggaatgc ttggcatgga tgaagtccat gatttcttga aaggctgtcg
    21001 tcatttatcg accggggaca ttgcaaatat ggcgtcttgc ttggtgaggc gattatgttt
    21061 tttcagtaac acttgggcac tgcttgattt gcatggtatt aggtcgagac cgtcattgcc
    21121 atcaagcttc gatttccatg tcaatcaggg attgacgtgg tctggtgttg acagcgccca
    21181 atcgatcgcg cgataggtat catcaggttg taccgaacat taagcgggtg cggttttatt
    21241 tatcgttttg atctgtgtca atgagcgttg aacttaagct gcatcagttg gaatgtcttg
    21301 ttgctgtcgc ggatcatggc agcgtacgaa aagcagcaaa ttacctcggt cgttcgccga
    21361 ccgcggtcag caacgcattg cgtgaacttg aattaaccgt ttgttccata ttggtggaac
    21421 gcaaaacgga aggggcggtc gtcacggatg ccggggctgc gttattggcc catgcccgcc
    21481 ttatattggc gcaattacag cgcgcctatg acgatgccgc gttgctggcg ctgcaaaatg
    21541 gcggaatggt gcgcatggcg gtcactccct ggctgatgca gggtgtattg ccccacgtta
    21601 tcaaggagtt ccgcgcccag cgtccggatg tgcagctcaa tatcaccgag cacttcggca
    21661 tcgagtatcc ggcggtgcgc agcggccagc tcgacctggc tttcggcccg cggcccgaca
    21721 gccagcatga ccacgcgctc gaggttacgc ccttatatag ttattcgtat gccgtggttt
    21781 gccgtcaggg tcatcccggt gaatcggcgc ggaccgtatc cgatctgacg ggctatgact
    21841 ggctgctcag ccggcccatc gaacacataa tgccggtcgt gcgcgacctg ataaaagccc
    21901 agacgcgcaa gaacgtctgc cattttcatt atgtgccgtc cctgcaggcc acgctggcga
    21961 tgatccggtc caccgacatg ctcgccatca tgccctggcc cctgctcgaa acgccggacc
    22021 tgcggcatcg cttcgtggcc ctggatatgt cccccatcat gcaggagtcg accacctgtc
    22081 tgatcacgcg ccgctatgac cccctgcagg ggccagtact tgcctttctg gacacgttca
    22141 ggcgggtcgc gcgcgacctc gccgcctcgg cggaccccga ggtgcggcgg atattcagca
    22201 tggtggaaag ccatatgtag cggcaataag aaaaaagcct cgcggctcat tcaaattgcg
    22261 aggctttcag tcggggcgat atgggttcgg cgcatcatcg cgcgcggacc agggccagcg
    22321 tccgcatcat ttcgttcttc aggatcatca agccttcgtc ggcgcccagg ccaggcttgc
    22381 acagcatgaa gtcggcgccg caggccaagc cgacctgcgc gcagatgcgc gcggactggt
    22441 cggtttcgtt tgccgtgccg cccaggcagg cgcccatgcc gcgggaccgg cagtacagca
    22501 ccgcctcgat ggagcgatgg atgccgccga ggtccggcgt cttgatctgg aggtagtcgg
    22561 cagcctggct gtcggcgaac agcttggcgt cttccaaggt gttgcaccac tcgtcggcga
    22621 tcaggccgat gtcgatgcca cggctgcgca gcgcctgcct cagttcgcgc agcgcgtcga
    22681 tctgggccga gcggctggcg cggatgacgg gcgattcgag cagcaggtgg tagggggccg
    22741 ccgccgcggc caggcgctcg aggtagtcca gcagcgcggc cggatcgggg aaggtctcgc
    22801 ccagcgtgcc gtagacgtcg aggtggatgc gcgggtggta gtccggttcg ccgatctcct
    22861 ggatgcgccg ggcgatgccg cgggcgtagt ccagcagctt ctcgccctgc ggaccgacgt
    22921 cgtcggggac catgaagttg gcgtgcggca gcaggtcgac ccgcttgagg atcatgcggt
    22981 cgagctgggc cgcgtcgtcg cgctggcacg aggccagcag cttgacgggt tcggtggcga
    23041 tcgtgctgcc gtagtcctgc gccaccactt cggtgatcgt gatcttgcgg gagagcgccg
    23101 cggcgtgcag cagcgcctgc gacacgccgt agcggatggc cgtgtgcagg ggccggccgt
    23161 cgggctggca gtcgtcgagc cgttgcgcgt tggcgcggaa gtcgtcggca tccaggcctt
    23221 gcagcagggg ccggatggcc aggtcgaggg cttgctggtg gtccgcggcg cgaaaggccg
    23281 ggtcgcggcc gccgaagccg gccaggatga cgtcggcgca gtcgccatgg gcaacctggt
    23341 cgtcctcgag gaccagcatc accgagacga tgtcggcggt ttccacgatg cggcgaaatc
    23401 ccggcgcaat gggatcgcct tcgtacagca cgccgttcct gcgcgcgccg gccttgatgg
    23461 cggccaggtc tcgatggaga tagccggaga tgccggtgct gaacaggacg cgggcgattt
    23521 tcatgtgggg ctctttggct tgggtgggca gttattggtg ggggcggtat cggatgcgcc
    23581 gacggttccg gctgccgtca acataccgtc cggcgccggc gcaggcaata gaccatccat
    23641 agaccacgcg agactgcaga agaagcaaaa gcccgcggaa ccccggttcc gcgggctttt
    23701 gctccggcgt gacagggatg cctggtcact ccggctggat gccggccgcg ttgacgacct
    23761 gcttccagcg cgcggcttcg tcggccacca gcttgccgta ttgcgcggaa gactggaggt
    23821 cgaggtcggc gccctgggcc tgcagccgtt ggaccagctc cgggtcttgc agggcctggt
    23881 cggcggcggc ttcccaggcg cgcagcacgt cagccggcgt gccgacgggc gcgtgcaggc
    23941 ccacccagcc gaagacggcg gccttgggat agcccagctc gacgacgctg ggcacatcgg
    24001 gcagggcggc gacccgcttc gggctggtga cggccagcgc gcgcagcttg cccgactgta
    24061 cctggggcac ggcggctggc agggacggcg tggccgtctg cgcctcgccc gagacgacgg
    24121 ccatgatggc ggggcccgcg cccttgtagg gcacatgggt ccattgcagg ccggtctgca
    24181 ggcgcagtgc ctcgccggcc aggtgcagcg gcgagccgat gccgccggag ctgaagttga
    24241 tcttgccggg attggccttg gtcagcgcga tcaattcggc cagggagcgc gccggcacgt
    24301 cggggttgac gaccaggacg tgcggcacgg tcacgaacag cgcgaccggc aggagatcct
    24361 tcagcggatc gacttgcatg gttcgataga ggctgggcgc cgtcgccagg ctggcgtcgc
    24421 tgaccagcag ggtatagccg tcaggggccg aacgggccac ataggacatg gcgatctggc
    24481 cgcccgcgcc gcccttgttc tcgaccacga tggaggcctt cattatcttg cccagggcct
    24541 gggcgatgta gcgcgccacc acgtcggtgg tgccgcctgg cgggtagggc accacccagg
    24601 tcacgggatg gtccgggtag gcggcgcggg cggccgccag cggcgcgcac agggcgagcg
    24661 cggccagggc gacgcgctgc agggttctgg aaagccggct catgttctgt ctcccaggcg
    24721 ggtttcgttg tcgcgcaagg ggctggacac ttcgtagagc aggccgtaga cggcgatgct
    24781 ttcggcgcgc cagccctgcg cggcgagcgg gccatcggca agcgccgtgc tgcgcagcgc
    24841 gtcggcctcg gtttcccgcg tcgtttcgac cagcagcgcg tagtcgacca ggtcgtcgga
    24901 ttgcccgacc gcgcgcacgg cgtccatgcg ttgccggacg gcgggctcgg cctgcagcca
    24961 gtgcacgccg accacgccgc gatggcgcag cagcccgagc gcgtaggcat ccagcgcgcc
    25021 cgcgtcggcc gggccgttgg ccttgagccg gtagctcgcc agcacggcgc ccaggccgcg
    25081 gcccgccgtg gcgcgtacgc ggaaggtgtt gcgcgccacg ttggaaaagc ccgcgcggat
    25141 gcgctgcgat tgcgggctga tgcgcttgac caggctgaga tagccctcgc tggccagcgc
    25201 ctcgggcgtg gtggcctcga acaccgacag atagccaggg gtgccggggg cgcgccgcag
    25261 cgcgcggtag cgtcgcgccc gcagatagcc gccgatgtcg atgcgttcgg gcaggtgttc
    25321 gtccacgtac cagtcgtcgg tctcgcggtc cagttccggc gacacgtcca gccacaatgc
    25381 caggacgccg cttcccatga gtgcgctcat gttttgctcc tcgtgtcgtt gttattcggc
    25441 ggtgatgccg gcgtgcttga tgacgccgga ccagagggcg tagtccttgc cgatggtggc
    25501 ggcgaattcc tggggcgtgg ccgtcatggt ttcggcgccc accagggcca gcgtctgctt
    25561 gagcgcggga tcggccacca cctggatcaa ggcctggtgc agcgtgtcga cgatggcggg
    25621 cggcgtgccg gccggggcga gcacgccgtt ccaggtcgtg acgaccaggt ccggcatgcc
    25681 ggcctcgcgg gcggtgggca cgtccggcag ggcagccgtg cgcttggcgc cggtgacggc
    25741 cagcgcgcgc agcttgccgc tcttgatcag cgacaccgcc gagggcagcg tcgcgaacag
    25801 ggcgtcgacc tggccgcccg cgacgtcgac cagggccggg ccgccaccct tgtacggaat
    25861 gtgcacgatg gacacgtcgg cctggctcct gaacagctcc gcggtcaggt ggttggtgct
    25921 gcctgtgccg gacgaggcga aggtcagggg cctggactgg gtcttggcct gcttgacgaa
    25981 gtcggccagg ctggcggcgg acaccgtggg gccgaccacc aggatgttgg gaaactccgc
    26041 caccggcatc actggcgcga aggccttggt gggatcgtag ggcagcgatt tgtagagcgt
    26101 ggggctcatc gcgatttcat tggtggtgcc gaacagcagc gtgtagccgt cgggcgccga
    26161 atgggcgacg taggccgcgc cgatggtccc ggtggcgccg ccgcggtttt cgatgatgac
    26221 gggctggccc agcaactgtc cgagcttctc ggccgtgggc cgggcgatga ggtcggccac
    26281 gccgccgggc gggaagggca cgaccaggtg gatgggcttg gacggatagg actgggccaa
    26341 tacggcggcc ggggccagga gcaggccggc cagccatgcg gccaggcggc cgcgcggtag
    26401 aaagaaaagg cgcatcgttt tgtctcctcg cgcatcgggg cgcgctatgg gtgttatgaa
    26461 ggacagcggg cggagtcagc ccgctggttc gggggcggcg aacaacccgg tcagtcgggc
    26521 gatgtccggg cagtcttcca ggttcagtac ggcctcgcgc agggcccggg cgcgaggcgg
    26581 ggtgagtacg ggatcggcat agccgtggaa tttcgcgtcg agctccgcgg cgtccagcgg
    26641 catcatggga tggccgggcg ggtagggaca atcgtgccgc cgcaccgtgc cgtcgcgcag
    26701 gcgcagctcg accccgccgc cggccgccgc cggccagcgc tcgttcagtg ccggatcttc
    26761 ggccagctcg accttggcga gcagcgcgcg caggtcgggc gcctgcagcc ggccgggctc
    26821 gaactgcgga tcgccgcagg cgccgtccag cagggcgacg gccgcgcagt agtagaagct
    26881 gtgctgcgcg ctttcgatat gggacgggta gcggcgatcg gggtgcgccg agggcgtgcc
    26941 cagcgcgaag cccggcagct tgacgatcat gcgctcgatg tcctcgaccc gcggcgcgcc
    27001 ttggcggtgc agcgcgatgg ccgcgcgtat cggcgccacc gccgtggcga ccgccggata
    27061 gggcttgatg ctgacttcca gcaggcggta gcgctcgccc atgggcgcgg tcagcgcgtc
    27121 gaagtccacc tggccggcga cgctggccgc ccagcccgac gcgccttcca tgagcggcag
    27181 cgggccggtg acgcccaggc cggccagcag cgcggcctcg acgcccgcct tggcgaccca
    27241 ggcttccgca gtggccttga tcatcgacac ggcgccgctt tgcagctgcg ccagcgtgtt
    27301 ctggtggctg cccgagatgg ccatggcgtg cgcgatgcgg acggggccgt ccccggccag
    27361 ccgcgcggcc agcgcggccg acgagaactg cgcgttggtg ccgtgatgcc agccgcgctt
    27421 ccacagcgag ggttcgcccg cgaaatcaca aaggcgcagg tggatctcgt aggcgaccgc
    27481 gagggccgcc agcagatcct tgccgctgcg gtgctcgcgt tcggccacgg ccagcgccgc
    27541 agccaggttg ccgctcgggt gggccgggtc gcgtgcgaag tagtagtcgt tgctgtccag
    27601 gaagcgcagc agcgtgccgt tgaccagcgt cgccagcgga gccgaggtct tgccgcccgc
    27661 gccgatcagc gtggcctgcg gcgcgccgcc gcagcttgcg gccatgcgca gcacggcctg
    27721 gccgacctcg ctgccgtagc cgccgtaggc gcagcccagc gtatccagga cgagggcctt
    27781 ggcccggtcg acgacctggc cgggcaggtc gtcgtagcgc acctcgtgca cccgccgggc
    27841 gagcgcttcc agcacgctgg tttcgtgctt gactgtcatg gcgctgtccc tggcctagaa
    27901 ggcgaaccgg ctggagcccc cgtccaccgg gatgactgtg ccggtgatgt aggcggcctg
    27961 ctccgaagcg aggaacatcg cgagcccggc gatttcctcg ggctcgccga agcggcccag
    28021 cgggatttcc gcgcgcgcgt attccagttc gctttccagc gtggggtagc gcttggtcat
    28081 ctgttcgctg tggatgcggc cgggctgtat gcagttcacc gtgatgccgg atgccccgac
    28141 ttcgcgcgac agccctttgg cccatacgtg cacggctgcc ttagccgtga aagcggcgtt
    28201 gagcatgcgc ggctcggacg tgccggtgaa ggtgaccacg cgtccccact tgttctcgcg
    28261 catgccgggt atgacggcgt gggtgagttc gcggatccgg aagaagttca gcaccatgcc
    28321 ctcgctccat tgctcgcgcg gggcttcgaa aggcacgggg cggctggcgc ccgcggcgtt
    28381 gatgaggatg tcgacgcggc ccatcaggcg ggcggcttcg gcggcgacgc gctcgggcgc
    28441 gtcttcgtcg tagaaatcgg ccgccagcgc gatgagccgg ccgccctggg ttgtatcgat
    28501 gccgtgcgcg acttgctcga ccagttcctt gcggcgggcc acgccgacca cggtggcgcc
    28561 gctttcggcc agcatgcgcg cggtggcgcg gccgatgccc tggctggctc cggtgaccag
    28621 ggcggt
//
LOCUS       DASWPO010000035        12144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2417737, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000035 DASWPO010000000
VERSION     DASWPO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417737"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            574..3660
                     /locus_tag="VGC69_01845"
     CDS             574..3660
                     /locus_tag="VGC69_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY0294057.1"
                     /translation="MKRMLFNATHQEELRVAIVDGQKLIDLDIETAGREQRKGNIYKG
                     VITRIEPGLEACFVNYGEDRHGFLPFKEVARTYFKEGVDVRTARIQDALKEGQELIVQ
                     VEKEERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRQELRDTMDQLDVP
                     QGMSIIARTAGIGRSVEELQWDMAYLMQLWTAIDGAARDNAAPILIYLESSLVIRAIR
                     DYFSPDIGEILVDTDEIADQAIAFMSVVMPDNVQRVKRYRDDVPLFSRFQIEHQIETA
                     YSRTVNLPSGGAIVIDHTEALVSVDVNSARSTKGSDIEETALRTNLEAADEAARQLRL
                     RDLGGLVVIDFIDMEEGKNQRAVEQRLRDALHFDRARVQMGKISRFGLMELSRQRLRP
                     ALNEGSHITCPRCNGTGVIRDAESSALHVLRLLQEESMKENTAAVHAQVPVEVATYLL
                     NEKRADISKIEARQKVNLVLIPNKHLETPHHHLERLRHDDPRLEETKTSFELVEAPST
                     IETWSPHAETEAKAKPEALVKGITPAQPAPVHTPAPTAAAAPAQAAAPVVGGWFKRLT
                     NWFTGGDTVDAPAAAPAEASEESRRGAGPNRGRRSTHDGQERRGERHGSERNRGRRNS
                     ETPVDQEAGASRPPHPRGGRRNEERTDRPPRSERTEQRAERDTAQAANRPERAPEAAV
                     GEEAATPRNGSRGRNRRGRGRREDGADQAPLSEQESMVAALAETVASALPDSKQAAAV
                     QPVADTLDEQEMETPDGLADQSAEAGADPERKRRRRRSRRGRRNADETTGLTAEGQDG
                     DIQDGDAQEQDDDLPAEPAPIAVAEPAPLVAVPVVAAPIAVAPVAAAPVVAAPVAEIE
                     PVPAAAPVAETVVVEAIVAAHAETQQAPAAFQTVQETAPVEPAPVVAAVAEPVVIEPV
                     VTAPVVAEPIVVEPVVAEPIAAAPAAPVEPVAAPAPIAIAPAPAAAEPAAPVQASTLH
                     GIVNAAGLTWVETDPARHVQTQQRIAASIVPVRLGRERKPVPQASTAPLQQVETSR"
     gene            complement(3796..4752)
                     /locus_tag="VGC69_01850"
     CDS             complement(3796..4752)
                     /locus_tag="VGC69_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY0294058.1"
                     /translation="MAVSAVALAATVAAGSAGAAGYPSQPIRIIIPFAPGGGADLPGR
                     LLAEKLSQAWGQSVVVENRPGATGSIAESFVARSAPDGYTVLLATPSSHTIGPHLLKN
                     ISFDPLEDLRGVTLFGWVPNVLVVNPSVPAGNLQELIALAKQKPGALNYSSSGQGSSV
                     QVTTETFAQQAGITLTHIPYNGVTPAVTAVISGQVQMMFAPAALALPQIKAGKLRQMT
                     PLVDTAKIDADWSRTPSQTVAQRNAANWLGFVMPARTPENVVRTWQDALATIMQSQDV
                     REKFAEWTIIPADTSGAPFDAIIKKEYQESGAMLKRLDIDPS"
     gene            5089..6219
                     /locus_tag="VGC69_01855"
     CDS             5089..6219
                     /locus_tag="VGC69_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY0294059.1"
                     /translation="MLRSVALRNRIVVSPMCQYASVDGAPTDWHLVHLGKFAIGGAGL
                     VFGEETAIDPLGRKTYECAGIWKDQHVAPYRRITDFIRQNGAVPAIQLGHAGIRASSH
                     GAMKNWIALTPENTTPDQPPWTVAGPSRPGDSPHWPRIREMTPGDIQASLATWKEATL
                     RALDAGFDVLEIHGAHGYLIHEFLSPVTNLRKDGYGGDRASRMRYALEVTETVRSAWP
                     AHLPLFFRLSAVDGKGGVWNVDDSVALAHCLKERGVDVIDVSSGGVSGPSDMPLVPRV
                     PGYQVGYASRIRNEAGVQTMAVGLITTAQQAEQILHAGQADLIAMARELMWDPQWPSR
                     AALELIGDEGFELLPEAYGYRLKRREAEARMAHNQPGTISQG"
     gene            complement(6230..7207)
                     /locus_tag="VGC69_01860"
     CDS             complement(6230..7207)
                     /locus_tag="VGC69_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294060.1"
                     /translation="MKLCLKNLAVACSALLLSAWALAAQAAFPDDGTIRFVVPFTSGG
                     NTDVVARIVAKGMSARLKAPVIVVNKPGANAMVGAEYVAHAKPDGYTMMLGTAESLAI
                     NPHIYAKVNYDALKDFAEVGVIGGFPFALVANPKLPVKTLADFVDYARKRPGQLTFSS
                     WGVGSTSQIAFEQFKQVTGIDMRHIAYQGAAPAITAVEAGDVDAFMVPLTVAVPQAQG
                     GKVKLLGITNAQREPSAPSIPTMTEQGYPVVIGGWHVLVVPRDTPPDVVRALNAALNA
                     ALEDKDVRGVLTAQGLDPAKTTPDEAAQWIRKEFDRWGSIATSAHVSGN"
     gene            complement(7261..7983)
                     /locus_tag="VGC69_01865"
     CDS             complement(7261..7983)
                     /locus_tag="VGC69_01865"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294061.1"
                     /translation="MIGWRARLGFLIPPGSPTTETEIIQMKPSGVSVHFTRMVVGATT
                     PGSLDGQEERNRSQIAHLGLNIELLSMVKPSVIALAHTATSYTLGKDEEAQLTRRVEA
                     ETGIRFITAFASVVAALRQLGVSRVAVGTPYSESTTQQCRLNLEAHGFQVVSCARLQG
                     VTNIYDETAERAYQLARQVDVAEAQAVFLSGVGMPTVDVLQTLEQDTGKPAISSTSAM
                     MWNALRTAGVQPSIPGYGRLLA"
     gene            complement(8096..8575)
                     /locus_tag="VGC69_01870"
     CDS             complement(8096..8575)
                     /locus_tag="VGC69_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY0294062.1"
                     /translation="MAQADGEGTQGGIGIHYGFGDRFSRFNLQYETPSVWTYSFGGNW
                     GRVDLTPEIGVAYWHAHSGNSPSSVWQLNAIPMFRWWTSEHFYIEAGAGPTLFSRVAF
                     AGRYYSSAFQFGDHIGVGYLIDKHNRIGVRISHFSNASIKEPNPGMTMAQVTYTYQF"
     gene            complement(8839..9717)
                     /gene="accD"
                     /locus_tag="VGC69_01875"
     CDS             complement(8839..9717)
                     /gene="accD"
                     /locus_tag="VGC69_01875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY0294063.1"
                     /translation="MSWIDKLLPPRINKASNENTARRVPEGLWVKCPSCESVLYSEDL
                     AANLHVCPKCDHHMRIGARTRLDMLLDLEGRVEIGSTTRSVDTLKFKDTRKYPERIAE
                     AIKQTGETDALVVMSGSIRSVPTVVAAFEFEFMGGSMGSVVGERFVRGVQAAIDSRTP
                     FICVAASGGARMQESLLSLMQMAKTNAMLTRLAAVGQPFISVLTDPTMGGVSASFAFM
                     GDVVIAEPKALIGFAGPRVIEQTVREKLPEGFQRSEFLLQKGAIDMVIDRRHLREELA
                     RLMALLNRQPVDVVAA"
     gene            complement(9752..10609)
                     /gene="trpA"
                     /locus_tag="VGC69_01880"
     CDS             complement(9752..10609)
                     /gene="trpA"
                     /locus_tag="VGC69_01880"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0294064.1"
                     /translation="MTQANRIDTTFQQLAAAGRAALVPYIAAGDPSPAATVPLMHALV
                     EAGADLIELGVPFSDPMADGPVVQRATERAIAQGMSLGRLLERVAEFRRDDKHTPVVL
                     MGYANPIERMGQQAFSQRAREAGVDGVLVVDYPPEEVTDFADQLGRDGIHPIFLLAPT
                     STEARIQAVARVARGYVYYVSLKGVTGSASLDTDDVAERLALIRRHVSIPVGVGFGIR
                     DAESAARVARHAEAVVIGSKLIETMEQAVAGAAPGKQDEAAVAAAGQWLRTIRAALDA
                     ARKQTSPAA"
     gene            complement(10620..11843)
                     /gene="trpB"
                     /locus_tag="VGC69_01885"
     CDS             complement(10620..11843)
                     /gene="trpB"
                     /locus_tag="VGC69_01885"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY0294065.1"
                     /translation="MPDSRRKVVKPYDLPDVHGHFGPYGGVFVAETLMHALDELREAY
                     DHARTDPAFIEAFNYELKHFVGRPSPVYHAERWSRELGGAQIWLKREDLNHTGAHKIN
                     NCIGQALVARRMGKTRVIAETGAGQHGVASATVAARYGMECVVYMGSEDIRRQAANVY
                     RMKLLGAKVVPVESGSRTLKDALNEAMRDWVTNIENTFYIIGTVAGPDPYPRMVRDFQ
                     TVIGKECLTQMPEAAGRQPDYVVAAVGGGSNAIGIFHPYIPHESVRLIGVEAAGEGIE
                     TGRHAAAIAAGRVGVLHGNRTYIMQNDDGQIIESHSVSAGLDYPGVGPEHAWLKDTGR
                     AEYVGITDDEALAAFHACCRIEGIMPALESSHAIAHAVKMAPALPRDQIILVNLSGRG
                     DKDMHTVAERSGITL"
BASE COUNT         2100 a         4105 c         3972 g         1967 t
ORIGIN      
        1 ggaaggttct ttgcgcattg cggaaagcaa ggatacaatc gggacagcct tgaggggctg
       61 aaccgcccac gccgtctata tggatagggc acggtttgac gttgagatgc gcgttgcatt
      121 gtcgaaaatg caattgagtt cgagcaaaat ccgaacccga cgcgcgctcc gtcgaacatg
      181 atgccgccat tgtactgcct gctggttcag ctccggggtc tctcggtcgg cggcgcttgt
      241 agcagccgca cgcgacctga agcacaagag aatcgtcgca atagtaatta cgcaccagat
      301 gcgtgcagtg cggagcgacc caagcccgcc cgccgcatct gacgttccca gcaactttcc
      361 tgtcatccac ttacgtgaaa ttgacctccc tgctgaaaga accgcgtgcc acctggcacg
      421 acgtgcccgc ctgacccgcg gcggatacgc cctggccgca tcactgcatg ggcccgggta
      481 cggtccgcgc tttctctgct tcagtttgcc gcccgattga tccgaggtca cgcgccgaac
      541 ccggcgtgtt cctgactacg gagaatcgca ctcatgaagc gcatgctgtt taacgcaacg
      601 caccaggaag aactgcgcgt tgccatcgtc gatgggcaaa aactcatcga cctcgacatc
      661 gaaaccgccg gacgcgaaca gcgcaaaggc aatatctaca agggcgtcat cacccgcatc
      721 gaacccggcc tcgaagcctg tttcgtcaac tacggcgaag accgccacgg cttcctgcct
      781 ttcaaggaag tcgcccgcac ctacttcaag gaaggcgtcg atgtgcgcac cgcgcgcatc
      841 caggacgcct tgaaggaagg ccaggaactc atcgtccagg tcgagaagga agagcgcggc
      901 aacaagggcg cggccctgac caccttcatt tcgctggccg gccgctacct ggtgctgatg
      961 cccaacaacc cgcgcggcgg cggcgtctcg cgccgcgtcg agggcgagga ccgccaggaa
     1021 ctgcgcgaca cgatggacca gctcgacgtg ccgcagggca tgagcatcat cgcccgcacc
     1081 gccggcatcg gccgcagcgt cgaagagctg caatgggaca tggcctacct gatgcagttg
     1141 tggacggcca tcgacggcgc cgcccgcgac aacgccgccc ccatcctgat ctacctggaa
     1201 tccagcctgg tcatccgggc catccgcgac tacttctcgc ccgatatcgg cgaaatcctg
     1261 gtcgacaccg acgagatcgc cgaccaggcc atcgccttca tgagcgtggt gatgccggac
     1321 aacgtgcagc gcgtcaagcg ctaccgcgac gacgtgccgc tgttctcgcg cttccagatc
     1381 gagcaccaga tcgagacggc ctactcgcgc accgtcaacc tgccctcggg cggcgcgatc
     1441 gtgatcgacc acaccgaggc cctggtgtcg gtcgacgtga actcggcccg ctccaccaag
     1501 ggatcggaca tcgaggaaac cgccctgcgc accaacctgg aagccgccga cgaagcggcc
     1561 cgccagttgc gcctgcgcga cctgggcggc ctggtcgtga tcgacttcat cgacatggaa
     1621 gaaggcaaga accagcgcgc cgtcgaacag cgcctgcgcg acgccctgca cttcgaccgt
     1681 gcccgcgtac agatgggcaa gatctcgcgc ttcggcctga tggagctgtc ccgccagcgc
     1741 ctgcgcccgg ccctgaacga aggctcgcac atcacctgcc cgcgctgcaa cggcaccggc
     1801 gtgatccgcg acgccgagtc cagcgccctg cacgtgctgc gcctgctgca ggaagagtcc
     1861 atgaaggaaa acaccgccgc cgtgcacgcc caagtgccgg tcgaggtggc cacctacctg
     1921 ctcaacgaaa agcgcgccga catctccaag atcgaagccc gccagaaggt caacctggtg
     1981 ctcatcccca acaagcacct ggaaaccccg caccaccacc tcgaacgcct gcgccacgac
     2041 gacccgcgcc tggaagaaac caagaccagc ttcgagctgg tcgaagcgcc cagcaccatc
     2101 gagacctggt cgccgcacgc cgaaaccgaa gccaaggcca agcccgaagc gctggtcaag
     2161 ggcatcaccc cggcccagcc ggcccccgtg cacacgcccg cccccactgc cgccgccgcg
     2221 cccgcccagg cggccgcccc cgtggtcggc ggctggttca agcgcctgac caactggttc
     2281 accggcggcg acaccgtgga cgccccggct gccgcgcctg ccgaggccag cgaagaatcg
     2341 cgccgcggcg ccggcccgaa ccgcggccgc cgtagtaccc atgacggcca ggaacgccgt
     2401 ggcgaacgcc atggttccga gcgcaatcgc ggccgccgca acagcgaaac ccctgtcgac
     2461 caggaagccg gcgccagccg tccgccgcat ccgcgcggcg gacgccgcaa cgaagagcgc
     2521 accgatcgcc ccccgcgcag cgagcgcacc gagcaacgcg ccgagcgcga cacggcccag
     2581 gccgccaatc gccccgagcg cgccccggaa gccgccgtgg gcgaggaagc tgccacgccc
     2641 cgcaacggca gccgtggccg caatcgccgc ggtcgcggtc gccgcgagga cggcgccgac
     2701 caggcgccgt tgagcgagca ggaaagcatg gtggcggccc tggccgaaac cgtggcctcc
     2761 gccctgcccg acagcaagca ggccgccgcg gtccagccgg ttgccgacac cctcgatgaa
     2821 caggagatgg aaacccccga cggactggcc gaccagtccg ccgaagccgg cgccgatccc
     2881 gaacgcaagc gccgccgccg ccgcagccgt cgcggccgcc gcaatgccga tgaaaccacg
     2941 ggcctgaccg ccgaaggcca ggacggcgac atccaggacg gcgacgccca ggaacaggat
     3001 gacgacctgc cggcagagcc cgcgccgatc gccgtcgccg aaccggcccc gctggttgcc
     3061 gtgccagttg ttgctgcgcc gattgctgtt gcccccgttg cagccgcacc ggttgttgcc
     3121 gcgccggttg ccgagatcga accggtcccg gctgccgcgc cggtcgctga aaccgtcgtg
     3181 gtcgaggcca ttgtggccgc gcacgccgag acccagcagg cgcccgccgc cttccagacc
     3241 gtgcaggaaa ccgcaccggt cgaaccggcg ccggtcgtgg cggccgtggc cgagcccgtt
     3301 gtgatcgagc ccgtggtgac cgcaccggtc gtggccgagc cgatcgtggt cgagccggtc
     3361 gtagccgagc cgatcgcggc ggcccccgcc gcaccggtcg aacccgttgc tgcgccggct
     3421 ccgatcgcca tcgcaccggc tccggccgcc gcagaaccgg ccgcgccggt ccaggccagc
     3481 acactgcacg gcatcgtcaa tgccgccggg ctgacctggg tggaaaccga tccggcccgc
     3541 cacgtccaga cccagcagcg catcgccgcc agcatcgtgc cggtgcgcct gggccgcgag
     3601 cgcaagcccg tgccgcaggc ctccaccgcg ccgctgcaac aggtcgagac gagccgctga
     3661 gcgtcattcg gcaagacagg cctggacact accgggcctg gcctgacgac aaaaaacccg
     3721 ctgggtgcat ccagcgggtt ttttcttgtg gatgacgtat ccggctgcag tagcggaggg
     3781 ccgagtggat gcgcgctacg aagggtcgat gtcgaggcgc ttgagcatcg ctccgctctc
     3841 ctggtattcc ttcttgatga tcgcgtcgaa aggcgcgccc gaggtgtcgg caggaatgat
     3901 ggtccattcg gcgaatttct ctcgcacgtc ctggctttgc atgatggtcg ccaacgcatc
     3961 ctgccatgtc ctgacgacgt tttcgggcgt cctggccggc atcacgaagc cgagccagtt
     4021 cgctgcgttg cgctgcgcca cggtctggct gggcgtcctg gaccagtcgg catcaatctt
     4081 ggcggtgtcg accaggggcg tcatttgcct gagcttgcct gccttgatct ggggcagggc
     4141 cagcgctgcg ggagcgaaca tcatctggac ctggccggag atgacggcag tcaccgcggg
     4201 agtgacgccg ttgtagggta tatgcgtcag cgtaatgccg gcctgctgcg caaacgtttc
     4261 ggtcgtaacc tgaaccgagc tgccctgccc cgacgacgaa tagttcaagg cgccgggctt
     4321 ctgtttcgcc aaggcgatca gctcctgcag attgccggcc ggcaccgatg gattgaccac
     4381 cagaacattc ggcacccaac cgaacaaggt gacgccgcgc agatcctcca aggggtcgaa
     4441 actgatgttc ttcaatagat gcgggccgat ggtatggctg ctgggcgtgg ccaggaggac
     4501 ggtgtagccg tccggtgcgg accgcgcaac gaagctctcg gcgatgctgc cggtggcgcc
     4561 gggacgattc tccaccacca ccgattgccc ccaggcctgg gatagtttct ccgcgagcaa
     4621 tcggcccggc aagtccgctc cgccccccgg agcgaaggga atgatgatcc ggatcggttg
     4681 cgaaggatag ccagccgccc cggccgagcc cgccgccacc gtcgccgcca gggcaacggc
     4741 actcaccgcc atccaacgct ttttcgtcgg cctattcgag ttctgtaacg tcaacataat
     4801 cgtccaacaa gttgcagcgc ggctgcggcg ccgatccggc gctcattgga cgatgggctg
     4861 tgatggcaag gaacaatcgt ttatctataa cgatatttcc ccggcgatgc gcatcgatgg
     4921 ccgccttcat cgcctaaata atttccgccg cttttcaaat ggcgagaatg tagatcgcaa
     4981 cgggaccttt gttgttaagg tgttcggctg tccccaaaca gcccaaggcg ccgcgacatg
     5041 ccgtgccggg cagcgagtac tcgagtgccc ctactgacaa gcccgttgat gctgcgctcg
     5101 gtcgcgctgc gcaatcggat cgtcgtttcc ccgatgtgcc agtacgcctc cgtggacggc
     5161 gcgccgaccg actggcatct cgtgcacctg ggcaaattcg ccataggcgg cgcgggcctg
     5221 gtgttcggcg aagagaccgc gatcgacccg ctaggccgca agacgtacga atgcgcgggc
     5281 atctggaaag accagcacgt tgcaccgtac cgccgcatca ccgatttcat ccgccagaac
     5341 ggcgccgtgc cagcgatcca gctcggccat gccggcatcc gcgcatcgtc tcatggcgcg
     5401 atgaagaact ggattgcgct gacgccggag aatacgaccc ccgaccaacc gccctggacc
     5461 gtggcgggcc cttcccggcc tggcgattcc ccgcactggc ccaggattcg ggaaatgacc
     5521 cctggcgaca tccaggcgag cctggccacc tggaaggaag ccacgctacg cgcgctcgac
     5581 gccggcttcg atgtattgga gattcacggc gcacacggtt atctgatcca cgagttcctc
     5641 tcgcctgtga ccaacctccg caaagacggg tatggcggcg atcgggcgtc tcgcatgcgc
     5701 tacgccctcg aagtaaccga aacggtgcgg tcagcctggc ctgcgcacct gcccttgttc
     5761 tttcgcctct cggccgtcga tggcaagggc ggcgtgtgga atgtcgacga ttccgtcgcc
     5821 ttggcgcact gcctaaaaga gcgcggcgtc gatgtgatcg acgtttcgtc cggaggcgtc
     5881 agcggtccat ccgatatgcc gcttgtaccg cgcgtgcccg gttatcaagt cggctacgcc
     5941 agccggatcc gcaatgaagc cggcgtgcag accatggcag tggggctgat caccacggcg
     6001 caacaagcgg agcagatcct gcatgcagga caggccgact tgatcgcaat ggcgcgcgaa
     6061 ctgatgtggg acccgcaatg gccgagccgg gccgccctcg aattgatcgg ggatgaaggt
     6121 ttcgagctct tgcccgaagc ctatggctac cgcctgaagc gccgcgaggc cgaggcgcgg
     6181 atggcgcaca atcagccagg cacgatcagc cagggttgac gagcgcgggc tagttgccgc
     6241 tgacgtgcgc cgaggtggcg atcgaacccc agcggtcgaa ctccttccga atccactgcg
     6301 cggcctcgtc cggcgtggtc ttggcgggat ccaggccctg ggccgtcagc acgccacgca
     6361 cgtccttgtc ttccaaggcc gcgttcagag ccgcgttcag cgcgcgcacc acgtccggcg
     6421 gcgtatcgcg cggcaccacc agcacatgcc agccgccgat gaccaccgga tagccctgct
     6481 cggtcatggt gggaatggac ggggcggaag gctcgcgctg cgcattggta atgcccagca
     6541 gcttcacctt gccgccctgg gcctgcggga ccgccacggt cagcggcacc atgaaggcat
     6601 cgacgtcgcc cgcctcgacc gccgtgatgg cgggcgccgc gccctggtag gcaatgtgcc
     6661 gcatgtcgat gcccgtgacc tgcttgaatt gctcgaaggc gatctgcgaa gtgctgccca
     6721 cgccccacga cgagaacgtg agctggcccg ggcgcttgcg ggcgtagtcg acgaaatcgg
     6781 ccagcgtctt gaccggcagt ttggggttcg ccaccagcgc gaacggaaac ccgccgatca
     6841 cgccgacctc ggcgaagtcc ttcaaggcat cgtaattgac cttggcgtag atgtgcgggt
     6901 tgattgccag cgactccgcg gtacccagca tcatcgtgta gccgtccggc ttggcatgcg
     6961 ccacgtattc ggccccgacc atggcattgg ccccgggctt gttcacgacg atgaccggcg
     7021 ccttcagccg cgccgacatg cccttggcca cgatgcgcgc aacgacgtcg gtgttgccgc
     7081 ccgaagtgaa gggcacgacg aagcggatgg tcccgtcgtc cggaaacgcg gcctgggcgg
     7141 ccagggccca ggcggacagc agcagcgccg agcaggccac tgccaggttc ttcaggcaaa
     7201 gcttcatgtc tcctccttgg cgcccgctcg atagccgggc gcgccggcct tgtttgttct
     7261 ttaagccagc agccggccgt agcccgggat ggacggctgc acgcccgccg tccgcaaagc
     7321 gttccacatc attgcagacg tgctggatat cgccggtttg ccggtgtcct gctccagggt
     7381 ctggagcacg tcgaccgtgg gcatgcccac gccgctgagg aagaccgcct gcgcctcggc
     7441 gacatccacc tggcgggcga gctggtaggc gcgttcggcc gtctcgtcat agatgttggt
     7501 cacgccttgc aggcgcgcgc agctgaccac ctggaagcca tgtgcctcca ggttgagccg
     7561 gcactgctgc gtcgtggact cgctataggg cgtgcccacc gccacccggc tgacgcccag
     7621 ttgccgcagg gccgcgacca cgctggcaaa agccgtgatg aagcgtatgc cggtctcggc
     7681 ctcgacgcgg cgcgtgagct gcgcctcttc gtccttgccc agcgtgtagc tggtggccgt
     7741 gtgcgccagg gcgatcaccg aaggcttgac catcgagagc agctcgatgt tcaagccgag
     7801 atgggcgatc tgcgaacggt tgcgttcctc ttgtccgtcc aggctgcctg gcgtggtggc
     7861 gcccaccacc atgcgcgtga aatggacgga cacgccggac ggcttcatct ggatgatttc
     7921 cgtctccgtc gtcgggctgc ctgggggaat caggaacccg agtctcgctc tccaaccgat
     7981 catgtcttct ccgtggcgcg ggccgcaatc cggttcttct tgtgtgatgg cctggattgt
     8041 aggcgccggc cgaccatgcg gtccaggcgc ggcgcagcaa gcgcccgccc cttgcctaga
     8101 actggtaggt gtaggtgacc tgcgccatcg tcatgcccgg attgggctcc ttgatgctgg
     8161 cattggaaaa gtgcgagatc cgcacgccaa tgcgattgtg cttgtcgatc aggtagccga
     8221 cgccgatgtg gtcgccgaac tggaaggccg aggagtaata ccggccggca aaggccacgc
     8281 ggctgaacag cgtgggcccc gcaccggcct cgatatagaa gtgctcgctg gtccaccagc
     8341 ggaacatggg gatggcattg agctgccaca ccgaggaagg actgttgccg ctgtgggcat
     8401 gccagtaggc cacgccgatt tcgggggtga ggtccacgcg gccccaattg ccgccaaagg
     8461 aatacgtcca gacggacggc gtttcatact gcagattgaa acgcgagaaa cgatcgccga
     8521 aaccatagtg aatgccgatg ccgccctggg tgccctcgcc gtcggcctgg gccatcgatg
     8581 caaaacctgc caacgccacc ccggcaaaac ccgccaggac ttttatcttg aaaccacgga
     8641 acatgaacac tccattcata tcaggtaatt cgtgacaaca ttaacagaaa tatatgtatg
     8701 taagccgaat acatatatgt tgaaccagcc gaacaatccc ggcatggtgc tgccgcgcca
     8761 catcgctggc caccccctcg cgccagcatg acaacggccg cccagcaggc ggccgttgca
     8821 cgtcatgcaa gggcaaagtc aggcggcgac cacgtcgacc ggctggcggt tcaggagcgc
     8881 catcaggcgg gccagttctt cgcgcaggtg gcggcgatcg atcaccatgt cgatggcgcc
     8941 cttctgcagc aggaactcgg agcgctggaa gccttcgggc agcttctcgc gcacggtctg
     9001 ttcgatcacg cgcggaccgg caaagccgat cagcgccttg ggctcggcga tgaccacgtc
     9061 gcccatgaag gcgaaactgg ccgacacgcc gcccatggtg gggtcggtga gcacgctgat
     9121 gaagggctgg ccgacggcgg ccaggcgcgt gagcatggca ttggtcttgg ccatctgcat
     9181 cagcgacagc aggctttcct gcatgcgcgc gccgcccgag gccgccacgc agatgaaggg
     9241 cgtgcggctg tcgatggcgg cctgcacgcc gcgcacgaaa cgctcgccca ccaccgagcc
     9301 catggaaccg cccatgaatt cgaattcgaa ggcggccacc acggtgggga cgctgcggat
     9361 ggaaccgctc atcaccacca gcgcatcggt ctcgcccgtc tgcttgatgg cctcggcgat
     9421 gcgctcggga tacttgcgcg tgtccttgaa cttgagcgtg tcgaccgaac gggtggtcga
     9481 gccgatctcg acgcggcctt ccaggtcgag cagcatgtcc aggcgggtcc gggcgccaat
     9541 gcgcatgtga tggtcgcact tggggcagac atgcaggttg gcggccaggt cttcgctgta
     9601 gagcaccgac tcgcacgacg ggcacttgac ccacaggcct tcgggcacgc ggcgagcggt
     9661 gttttcgttg ctggccttgt tgatgcgggg cggcaggagt ttgtcgatcc agctcattgt
     9721 gtctttttct ctagtctagg gcgcgggcgg ctcaggccgc cggcgaagtc tgtttgcggg
     9781 cggcgtccag cgccgcgcgg atggtgcgca gccactgccc cgcggccgcc acggcggcct
     9841 cgtcctgctt gccgggcgcg gcgccggcca cggcctgctc catggtttcg atgagcttgc
     9901 tgccgatcac cacggcttcg gcatggcgcg ccacgcgggc cgcgctttcg gcgtcgcgga
     9961 tgccgaagcc cacgcccacg ggaatgctca cgtggcggcg aatcagcgcc aggcgttcgg
    10021 ccacgtcgtc ggtgtccagg ctggccgagc ccgtgacgcc cttgagcgac acgtagtaga
    10081 catagccgcg cgccacgcgg gccacggcct ggatgcgcgc ctcggtcgaa gtcggcgcca
    10141 gcaggaagat ggggtggatg ccgtcgcggc cgagctggtc ggcgaaatcg gtgacttctt
    10201 cgggcggata gtcgaccacc agcacgccgt ccacgccggc ctcgcgggca cgctgcgaga
    10261 aggcctgctg ccccatgcgc tcgatggggt tggcatagcc catcagcacc accggcgtgt
    10321 gcttgtcgtc gcggcggaac tcggccacgc gctcgagcaa gcgccccagg ctcatgccct
    10381 gagcgatggc gcgctcggtg gcgcgctgga cgacggggcc gtcggccatg gggtcggaga
    10441 acggcacccc cagttcgatg aggtcggcgc cggcctcgac cagcgcgtgc atcaggggga
    10501 cggtggcggc gggcgaggga tcgcccgccg cgatgtaggg caccagcgcg gcgcggccgg
    10561 cggcggccag ttgctggaaa gtcgtatcga tgcggttcgc ttgggtcata cgggttccgt
    10621 cagagcgtga tgcccgatcg ttcggccacg gtatgcatgt ccttgtcgcc ccggcccgag
    10681 aggttgacca ggatgatctg gtcgcggggc agcgcggggg ccatcttgac cgcgtgcgcg
    10741 atggcgtgcg aggactccag cgcgggcatg atgccttcga tgcggcagca ggcatggaaa
    10801 gccgccagcg cctcgtcgtc ggtgatgccc acgtactcgg cgcggccggt gtccttcagc
    10861 caggcatgct cggggcccac gccagggtag tccaggcccg cggacacgga atgggactcg
    10921 atgatctggc cgtcgtcgtt ctgcatgatg taggtgcggt tgccgtgcag cacgcccacg
    10981 cggccggcgg cgatcgccgc ggcgtggcgc ccggtttcga tgccctcgcc cgccgcctcg
    11041 acgccgatca ggcgcacgga ctcgtgcggg atataaggat ggaagatgcc gatggcgttc
    11101 gagccgccgc ccacggccgc caccacgtag tcgggctggc ggccggcggc ctcgggcatc
    11161 tgcgtcaggc attccttgcc gatgacggtc tggaagtcgc gcaccatgcg gggatagggg
    11221 tcgggacccg ccaccgtgcc gatgatgtag aacgtgttct cgatattggt gacccagtcg
    11281 cgcatggctt cgttgagcgc gtccttcagg gtgcgcgagc cggattccac cggcacgacc
    11341 ttggcgccca gcagcttcat gcggtagacg ttggcggcct ggcggcggat gtcttcgctg
    11401 cccatgtaga ccacgcattc catgccgtag cgggccgcca ccgtggccga ggccacgccg
    11461 tgctggccgg cgccggtctc ggcgatgacg cgggtcttgc ccatgcggcg ggccaccagg
    11521 gcctggccga tgcagttgtt gatcttgtgg gcgccggtgt ggttcaagtc ttcgcgcttg
    11581 agccagatct gggcgccgcc cagctcgcgc gaccagcgct cggcgtggta gacggggctg
    11641 ggccggccga cgaagtgctt gagttcgtag ttgaacgcct cgatgaaggc cggatcggtg
    11701 cgggcgtggt cgtaggcctc gcgcagctcg tccagggcat gcatgagcgt ttcggccacg
    11761 aacacgccgc cgtagggacc gaaatgacca tggacgtcgg gcaggtcgta aggtttcaca
    11821 acttttctcc gcgaatcggg cattttaccg catggaggct ggaaccgagc tcgtgttcaa
    11881 gtaccgggat atctgagttc ttaagacgtc ctgcgggggg tggtggctcc ctgtggggct
    11941 tcgctgtgcc gggctcgggc gcatgcaggc gccctcggtc ccctgcgggg actcccccgt
    12001 cccagaggcg ccccacaggg agccaccacc ccccgcagga ctggcagcag agccagaagc
    12061 gggacgtcaa cagtagcgga tttgaagccg tcgcatggat ccggtgcagt ttcccggctg
    12121 cggggtcggc ctgttcccag gggc
//
LOCUS       DASWPO010000036         7912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1345835, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000036 DASWPO010000000
VERSION     DASWPO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345835"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VGC69_01890"
     CDS             complement(<1..741)
                     /locus_tag="VGC69_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY0294066.1"
                     /translation="MDASATQRIVKIRRDYNTWVANETLEDYALRFTPVSFRKWSEYR
                     VANTALGAVSFLALEAIGGSLALNYGFVNAFWAIMVVSLLVAMTSLPIAYYAARYGLD
                     MDLLTRGAGFGYIGSTITSLIYASFTFIFFAVEASIMALAIELSTGLPLAWGYLVCAV
                     AIIPFVTYGVTLINRLQSWTQPVWLVLLALPYICVLLRNPEELAGLTHFAGLTSDGGH
                     FNLLMFGAGAAVAASLVTQIGEQVDFLRF"
     gene            952..2214
                     /gene="urtA"
                     /locus_tag="VGC69_01895"
     CDS             952..2214
                     /gene="urtA"
                     /locus_tag="VGC69_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEY0294067.1"
                     /translation="MSRKPDSEKDLASPSRRRAALTLASLPLMGIPALGRAAGPATSA
                     VNTTGLAITDTEVTVGQLHSATGTMAISETGSIQAERLAIEQINAAGGILGRQIKIIQ
                     EDGASDWPTFAEKARKLLVSDKVATVFGCWTSASRKAVLPVFEKENGLLYYPTFYEGL
                     EQSHNVFYTGQEATQQILASLNWLAKEKGAKTFYLVGSDYIWPRTSNKIARKHIENVL
                     KGTVVGEEYYALGDTQFGSLINKIKLRKPDIVFADVVGGSNVAFYKQLKAAGVTAEKQ
                     KLLTISVTEDELLGIGGENAEGFYSCMKYFQSLKNDNNTKFVAAFKAKYGPTAVIGDV
                     SQAAYLGPWLWKMAVEKAQSFDVDKVVAASPGLEFKEAPEGYVKIHENHHLWSRTRIG
                     QIRKDGQFDVVYETPDLIEPNPFPKGYQ"
     gene            2307..3224
                     /gene="urtB"
                     /locus_tag="VGC69_01900"
     CDS             2307..3224
                     /gene="urtB"
                     /locus_tag="VGC69_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEY0294068.1"
                     /translation="MDSLSDLGAILLMQGFNGLSVFSVLLLMALGLALIFGQMGVINM
                     AHGEFLAVGAYVTYILSVLTETHAPGLMPYYFFCAIAAAFIVTFALGWLVEWLMIRHL
                     YRRPLDTLLATWGLSLVMQQALRSIFGPREVSPTLPDWLMGSVQPLADVDIPINGLTV
                     MVITVVLTAGLLIALFHSSWGLHVRATMQNRVMSGAVGIDTRKVDRVTFALGCGIAGI
                     AGAAFTTIGSTGPTSGSLYIVDTFLTVVFGGAASLYGTIASAFVIAQTNSLAGFFLSG
                     SMAKVLTLSAVVIILMLRPQGLFTIKVRK"
     gene            3244..4380
                     /gene="urtC"
                     /locus_tag="VGC69_01905"
     CDS             3244..4380
                     /gene="urtC"
                     /locus_tag="VGC69_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEY0294069.1"
                     /translation="MNSFYKNVLGGRQGLIGLVILAVLIFAVFPLALDGFRLNLVGKY
                     LTYAFVAVGLVLCWGYGGILSLGQGVFFGLGGYCMAMFLKLEASTPEATKIQSTPGIP
                     DFMDWNQITSLPWFWVPFKSFLFTLFAVPIVPMLLALVLGAAMFKRRVGDTYFAIVTQ
                     AVALILSVLIVGQQGWTGGVNGITDLKTLLGWDIRTDSAHVILYFVNGALLFACILFG
                     RFLLASKLGKLLVAMRDKEDRVRFSGYDVASFKIFVFCVAAAFSAIGGAMFTLQVGFM
                     SPSFVGIVPSIEMVIFAAVGGRLSLLGAVYGTLLVNYGKTVFSESFPQLWLFLMGGLF
                     IAVVMAFPRGLAGLYEEYVKPRLGRAAPGKAAAENGSTNSTEAA"
     gene            4377..5141
                     /gene="urtD"
                     /locus_tag="VGC69_01910"
     CDS             4377..5141
                     /gene="urtD"
                     /locus_tag="VGC69_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY0294070.1"
                     /translation="MTAINAVPKPQEFALYIEGLTVSFNGFVAVNDLNLYVDKGELRV
                     VIGPNGAGKTTVLDLICGRTKATSGSIRFKDAELTKMAEHQIVRAGVGRKFQTPSIYE
                     TLSVRENLEVSFPRGRSVFGAMTFRRTPDVVERVEKTAQDIFLSDMLDQSAELLSHGQ
                     KQWLEIGMLLMQEPELLMLDEPVAGMSVSEREKTAELLNRISKNRSMIVIEHDMEFVK
                     NIADKVTVLHQGKVLAEGSMETVQADPRVIEVYLGH"
     gene            5168..5860
                     /gene="urtE"
                     /locus_tag="VGC69_01915"
     CDS             5168..5860
                     /gene="urtE"
                     /locus_tag="VGC69_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEY0294071.1"
                     /translation="MFSVSGLASGYGQSRVIHGIDLDVAEGEIVAVMGRNGMGKTTLF
                     KTMMGILPTMQGQVRIDGRDITGLETHQRVRSGVAYVPQGRMIFPTLTVEENIRTGLP
                     GRALRDPIPDEIFSLFPVLHEMRHRRGGNLSGGQQQQLAIARALVTGPKVLMLDEPTE
                     GIQPSIIKDIARSLLEIRKLKNLAIVVSEQVLSFTMQIADRLIVIDKGRFVHEDARDA
                     VDEQTISRYLSV"
     gene            5921..7150
                     /gene="fmdA"
                     /locus_tag="VGC69_01920"
     CDS             5921..7150
                     /gene="fmdA"
                     /locus_tag="VGC69_01920"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HEY0294072.1"
                     /translation="MPKTLIEVDLKQSPYDNENIHNRWHPDIPIAAWVKPGDDFVLET
                     FDWTGGAIKNDDSADDVRDVDLSTVHFLSGPVGVEGAEPGDLLVVDFLDIGAKPDSLW
                     GFNGFFSKKNGGGFLDEHFPLAQKSIWDFEGMFTQSRHIPGVRFAGLIHPGLIGCLPD
                     QALLDTWNQREQALIDTHPTRVPPLANPPAPQTAHMGKLKGAARDKAAAEGARTVPPR
                     EHGGNCDIKDLSRGSRVFFPVYVKGGGLSVGDLHFSQGDGEITFCGAIEMAGWIHMRV
                     SLIKDGMAKYAIKNPIFKPSPITPNYKDYLIFEGISVDEKGGQHYLDVTVAYRQACLN
                     AIEYLKKFGYSGAQAYSILGTAPVQGHVSGVVDIPNACATLWLPTEIFDFDIMPSAGG
                     PVQAIGGGVDLPLSPDL"
     gene            7164..7499
                     /locus_tag="VGC69_01925"
     CDS             7164..7499
                     /locus_tag="VGC69_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0294073.1"
                     /translation="MPTYDYSCARCGAFSALRPLAQRHDAASCPQCGAAAPRVLGAAP
                     ALGALSSAMNRARAMNERNANEPRSARAGHGMHCGCCGGGKKLPGKTRATADGGKTFA
                     GSRPWMISH"
     gene            7662..>7912
                     /locus_tag="VGC69_01930"
     CDS             7662..>7912
                     /locus_tag="VGC69_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0294074.1"
                     /translation="MPTSSHAAPALRFEGTDRYVATDDLKLAVNAALTLQRPLLIKGE
                     PGTGKTMLAEEVARALDRPLLQWHIKSTTKAQQGLYEYDA"
BASE COUNT         1405 a         2788 c         2434 g         1285 t
ORIGIN      
        1 gaagcgcagg aaatccacct gctcgccgat ctgcgtcacc agggaggcgg ccaccgccgc
       61 cccggcgccg aacatcagca ggttgaaatg gccgccgtcg ctggtcagcc cggcgaaatg
      121 cgtcagcccc gccagctcct ccggattgcg cagcagcacg cagatgtagg gcagcgccag
      181 cagcaccagc cagaccggct gggtccagga ctgcaggcga ttgatcaggg tgaccccata
      241 cgtgacgaag ggaatgatgg ccacggcgca gaccaggtag ccccacgcca gcggcaggcc
      301 ggtggaaagc tcgatggcca gcgccatgat ggacgcttcc acggcgaaga agatgaaagt
      361 gaacgaggcg tagatcagcg aggtgatggt cgagccgatg tagccgaagc cggcgccgcg
      421 cgtcagcaga tccatgtcca ggccgtagcg cgccgcgtaa taggcgatgg gcaggctggt
      481 catggccacc agcagcgaca ccaccatgat ggcccagaac gcgttgacga aaccgtaatt
      541 cagcgccagc gacccgccga tggcctccag ggcgagaaag gacaccgcgc ccagtgccgt
      601 gttggccacc cggtactcgg accatttgcg gaaagacacc ggcgtgaaac gcagcgcata
      661 gtcttccagg gtctcgttgg ccacccaggt gttgtagtcg cggcgaatct tgacgatgcg
      721 ttgggtggcg ctggcgtcca cgcgtatcgc tcctgtatgc gtcgattgac gtatgccgtg
      781 tttgtactgc ctgggagagc ggcggccact acgtcaatcg acgtatgttg cagcgcgcca
      841 tgcgttccta gtattgcatc gaacaccccc ggaacgtgat gcgatccaag cgtaatcgac
      901 gcgatttcgg ggttttccct agcgtcttac atgcccccaa aggagcgttc gatgtcccgc
      961 aaacccgatt cagaaaaaga tcttgcgtcg ccgtcgcgcc gccgcgccgc gctgacgctg
     1021 gcatcgctgc cgctgatggg cattcccgcc ctcggccgcg ccgccggtcc ggccacgtcg
     1081 gccgtcaaca cgacgggcct cgccattacc gacaccgaag tcaccgtggg ccagctgcac
     1141 tcggccacgg gcaccatggc catcagcgaa acgggttcca tccaggccga gcgcctggcc
     1201 atcgagcaga tcaacgccgc gggcggcatc ctcggccgcc agatcaagat catccaggaa
     1261 gacggcgctt ccgactggcc caccttcgcc gaaaaagccc gcaagctgct ggtgagcgac
     1321 aaggtcgcca ccgtgttcgg ctgctggacc tcggcctcgc gcaaggccgt gctgccggtg
     1381 ttcgaaaaag agaacggcct gctgtactac cccactttct acgaaggcct ggaacagtcc
     1441 cacaacgtgt tctacaccgg ccaggaagcc acgcagcaga tcctggcgag cctgaactgg
     1501 ctggccaagg agaagggcgc caagacgttc tacctggtgg gctccgacta catctggccg
     1561 cgcacctcca acaagatcgc ccgcaagcac atcgaaaacg tgctcaaggg cacggtggtc
     1621 ggcgaggaat actacgcgct gggcgacacc cagttcggct cgctgatcaa caagatcaag
     1681 ctgcgcaagc ccgacatcgt gttcgccgac gtggtgggcg gcagcaacgt ggcgttctac
     1741 aagcagctca aggccgccgg cgtgaccgcc gagaagcaga agctgctgac catctcggtg
     1801 accgaggacg aactgctggg catcggcggc gagaacgccg aaggcttcta ctcgtgcatg
     1861 aagtacttcc agagcctgaa gaacgacaac aacaccaagt tcgtcgcggc cttcaaggcc
     1921 aagtacggcc cgactgccgt gatcggcgac gtgtcgcagg ccgcctacct cggcccctgg
     1981 ctgtggaaga tggccgtcga gaaggcccag agcttcgacg tggacaaggt cgtcgccgcc
     2041 tcgcccggcc tggagttcaa ggaagcgccc gaaggctacg tgaagatcca cgagaaccac
     2101 cacctctgga gccgcacccg catcggccag atccgcaagg acggccagtt cgacgtggtg
     2161 tacgaaacgc ccgacctgat cgaacccaat cccttcccca agggctacca gtaagccccc
     2221 ctggccgacc ccggccgctc ggggccggcg gcccgaccgc cgccggcccg tttttttgac
     2281 gcacccacgc tgcacgaggc acggacatgg actctctatc cgatctaggc gcgatcctgc
     2341 tgatgcaggg cttcaacggg ctttccgtat tcagcgtcct gctgctgatg gccctcggac
     2401 tggccctcat cttcgggcag atgggcgtga tcaacatggc gcacggcgaa ttcctggccg
     2461 tgggcgccta cgtcacctac atcctctccg tgctgacgga gacgcatgcc ccgggcctca
     2521 tgccctacta cttcttctgc gccatcgcgg cggccttcat cgtcaccttc gcgctgggct
     2581 ggctggtcga atggctgatg atccgccacc tgtaccgccg cccgctcgac acgctgctgg
     2641 ccacctgggg cctgtcgctg gtcatgcagc aggcgctgcg ctcgatcttc ggtccgcgcg
     2701 aagtcagccc caccctgccc gactggctga tgggctcggt gcagccgctg gccgacgtgg
     2761 acatccccat caacggcctg acggtgatgg tcatcaccgt cgtcctgacg gccggcctgc
     2821 tgatcgcgct gttccactcc agctggggcc tgcacgtgcg cgccacgatg cagaaccggg
     2881 tgatgagcgg cgccgtcggc atcgacaccc gcaaggtcga ccgcgtgacc ttcgcgctgg
     2941 gctgcggcat cgccggcatc gccggggcgg ccttcaccac catcggctcc accggcccga
     3001 ccagcggctc gctctatatc gtcgacacct tcctcaccgt ggtcttcggc ggcgcggcca
     3061 gcctgtacgg caccatcgcc tcggccttcg tcatcgccca gaccaattcg ctggccgggt
     3121 tctttctttc gggttccatg gccaaggtgc tcaccttgtc cgcggtggtc atcatcctga
     3181 tgctgcgccc gcaggggctc ttcaccatca aggtccgcaa ataagccgtt cgggagcacg
     3241 aaggtgaatt ctttctacaa gaacgtattg gggggccggc aaggactgat cggactggtc
     3301 attctcgccg tgctgatttt cgcggtcttc ccgctggcgc tggacggctt tcgcctgaac
     3361 ctggtgggca agtacctgac ctacgccttc gtggcggtgg gcctggtgct gtgctggggc
     3421 tacggcggca tcctgagcct gggccagggc gtgttcttcg ggctgggcgg ctattgcatg
     3481 gccatgttcc tcaagctcga ggcctcgacg cccgaggcca ccaagatcca gtccacgccg
     3541 ggcattcccg acttcatgga ctggaaccag atcacgtccc tgccctggtt ctgggtgccc
     3601 ttcaagagct ttctcttcac cctgttcgcc gtgcccatcg tgcccatgct gctggccctg
     3661 gtgctgggcg cggcgatgtt caaacgccgc gtgggcgaca cctatttcgc catcgtcacg
     3721 caggcggtcg ccctgatcct gtcggtgctc atcgtcgggc agcagggctg gacgggcggg
     3781 gtcaacggca tcaccgatct caagacgctg ctgggctggg acatccgcac cgattcggcg
     3841 cacgtgatcc tgtatttcgt caacggcgcg ctgctcttcg cctgcatcct gttcggccgc
     3901 ttcctgctcg cctccaagct gggcaagctg ctggtggcca tgcgcgacaa ggaagaccgg
     3961 gtgcgcttct cgggctacga cgtcgccagc ttcaagatct tcgtgttctg cgtggcggcg
     4021 gcgttctcgg ccatcggcgg cgccatgttc acgctgcagg tcggcttcat gtcgccttcc
     4081 ttcgtgggca tcgtgccttc catcgagatg gtgatcttcg ccgccgtggg cggccggctg
     4141 tcgctgctgg gcgcggtcta cggcaccctg ctggtcaact acggcaagac cgtgttctcc
     4201 gagagcttcc cgcagctctg gctgttcctg atgggcggcc tgttcatcgc ggtcgtcatg
     4261 gccttcccgc gcggcctggc cggcctgtac gaagaatacg tcaagccccg gctgggccgc
     4321 gccgcgcccg gcaaggccgc ggccgagaac ggctccacca actccacgga ggcggcatga
     4381 cagccatcaa cgcggtgccc aagccgcagg aattcgccct gtacatcgaa ggcctgaccg
     4441 tctcgttcaa cggcttcgtg gccgtcaacg acctgaacct gtacgtggac aagggcgagc
     4501 tgcgcgtggt gatcggcccc aacggcgccg gcaagaccac cgtgctcgac ctgatctgcg
     4561 gccgcaccaa ggccacctcg ggctcgatcc gcttcaagga cgccgagctc accaaaatgg
     4621 ccgagcacca gatcgtccgc gccggagtgg gccgcaagtt ccagacgccg tccatctacg
     4681 aaacgctcag cgtgcgcgag aacctcgaag tttccttccc ccggggccgc agcgtgttcg
     4741 gcgccatgac cttccggcgc acgcccgacg tggtcgagcg ggtcgagaag acggcgcagg
     4801 acatcttcct gtccgacatg ctcgaccaat ccgccgagct gctgtcgcac ggccagaagc
     4861 aatggctgga aatcggcatg ctgctcatgc aggagcccga gctgctgatg ctcgacgagc
     4921 ccgtcgccgg catgagcgtc tccgagcgcg agaaaaccgc cgaactgctc aaccgcatca
     4981 gcaagaaccg ctccatgatc gtcatcgagc acgacatgga gttcgtcaag aacatcgcgg
     5041 acaaggtcac cgtgctgcat cagggcaagg tgctggccga gggcagcatg gaaacggtgc
     5101 aggccgatcc gcgcgtcatc gaagtctatc tgggtcactg acccgcgcca tcgcaaggga
     5161 gccgcatatg ttcagcgtat cgggactggc atcgggctac ggccagagcc gcgtgatcca
     5221 cggcatcgac ctcgatgtcg ccgagggcga gatcgtggcc gtcatgggcc gcaacggcat
     5281 gggcaagacc acgctgttca agaccatgat gggcatactg cccaccatgc agggccaggt
     5341 gcggatcgac ggccgcgaca tcaccggcct ggaaacccac cagcgcgtgc gctccggggt
     5401 ggcctacgtg ccccagggcc gcatgatctt ccccaccctc acggtggaag agaacatccg
     5461 caccggcctg cccggccgcg cgctgcgcga ccccattccc gacgagatat tcagcctctt
     5521 tccggtgctc cacgagatgc ggcaccggcg cggcggcaac ctctcgggcg gccagcagca
     5581 gcagttggcc atcgcgcggg cgctggtcac gggccccaag gtgctgatgc tcgacgagcc
     5641 caccgagggc atccagccct ccatcatcaa ggacatcgcc cgttcgctgc tcgagatccg
     5701 caagctcaag aacctcgcca tcgtcgtatc ggagcaggtg ctgagcttca ccatgcagat
     5761 cgccgaccgc ctcatcgtca tcgacaaggg ccgcttcgtg cacgaagacg cgcgcgacgc
     5821 cgtcgacgag cagaccatca gccgctacct gtccgtctag ggcccgcgcg ccggcccttt
     5881 ccccgcatcc atcaccctcg caacgccaaa ggagatcgcc atgcccaaga ccctgatcga
     5941 agtcgacctc aagcaatccc cctacgacaa cgagaacatc cacaaccgct ggcaccccga
     6001 cattcccatc gcggcgtggg tcaagcccgg cgacgatttc gtgctcgaga ccttcgactg
     6061 gacgggcggc gccatcaaga acgacgacag cgccgacgac gtgcgcgacg tggacctctc
     6121 caccgtgcat ttcctgtcgg gcccggtggg cgtcgagggc gccgagccgg gcgacctgct
     6181 ggtggtcgac ttcctcgaca tcggcgccaa gcccgacagc ctgtggggct tcaacggctt
     6241 tttcagcaag aagaacggcg gcggcttcct ggacgagcac ttccccctgg cccagaaatc
     6301 gatctgggac ttcgaaggca tgttcaccca gtcgcgccac atacccggcg tgcgcttcgc
     6361 cggcctgatc catcccggcc tgatcggctg cctgcccgac caggccctgc tcgacacctg
     6421 gaaccagcgc gagcaggcgc tgatcgacac ccatcccacc cgcgtgccgc cgctggccaa
     6481 cccgcccgcc ccccagaccg cccacatggg caagctcaag ggcgccgcgc gcgacaaggc
     6541 cgccgccgaa ggcgcccgca ccgtgccgcc gcgcgagcac ggcggcaact gcgacatcaa
     6601 ggatctttcg cgcggctcgc gcgtgttctt cccggtctac gtcaagggcg gcggcctgtc
     6661 ggtgggcgac ctgcacttct cgcagggcga cggcgagatc accttctgcg gcgccatcga
     6721 aatggccggc tggatccaca tgcgcgtctc gctcatcaag gacggcatgg ccaagtacgc
     6781 catcaagaac ccgatcttca agcccagccc catcaccccc aactacaagg actacctgat
     6841 cttcgaaggc atctcggtcg acgaaaaagg cggccagcac tacctggacg tcaccgtggc
     6901 ctaccgccag gcctgcctga atgccatcga atacctcaag aagttcggct actcgggcgc
     6961 gcaggcctat tccatcctgg gcacggcgcc ggtgcagggg cacgtgagcg gggtggtcga
     7021 catcccgaac gcctgcgcca cgctctggct gccgaccgag atcttcgact tcgacatcat
     7081 gccttcggcc ggcggcccgg tgcaagccat cggcggcggc gtcgacctgc cgctgtcgcc
     7141 cgacctgtaa gcggagcgcc gccatgccca cttacgacta ttcgtgcgcg cgctgcggcg
     7201 ccttttcagc cctgcgcccg ctggcgcagc ggcacgacgc cgcgtcctgc ccgcaatgcg
     7261 gcgcggccgc gccccgcgtg ctgggcgccg cgcccgcgct gggcgcgctg tcctcggcga
     7321 tgaatcgcgc ccgcgccatg aacgagcgca acgccaacga gccgcgcagc gcgcgcgccg
     7381 gccacggcat gcattgcggc tgctgcggcg gcggaaaaaa actgcccggc aagacccgcg
     7441 ccacggccga cggcggcaag accttcgccg gctcgcggcc ctggatgatc agccactgag
     7501 gcgcaagcgc cgggcaggcc cggcgcgccg gttgcgaacg gcggcgctgc cctgccgcgg
     7561 acggcggaaa tcccggccgg gcggcaaaat gtacgataat cgctagagat tcaagcgctg
     7621 gcgggcttcg aaccgccttt tcgtacacgc ccccccagac catgcccacc tcctcccacg
     7681 ccgcgcccgc cctccgcttc gaaggcaccg accgctacgt cgccactgac gacctcaaac
     7741 tggccgtcaa cgcggccctc accctgcagc gcccgctgct gatcaagggc gagcccggca
     7801 ccggcaagac catgctggcc gaagaagtgg cccgcgccct ggaccggccg ctcttgcagt
     7861 ggcacatcaa gtccaccacc aaggcgcagc agggcctgta cgaatacgac gc
//
LOCUS       DASWPO010000037        10089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2060928, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000037 DASWPO010000000
VERSION     DASWPO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060928"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /gene="pyrF"
                     /locus_tag="VGC69_01935"
     CDS             <1..121
                     /gene="pyrF"
                     /locus_tag="VGC69_01935"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416271.1"
                     /note="type 2 subfamily; involved in last step of
                     pyrimidine biosynthesis; converts orotidine 5'-phosphate
                     to UMP and carbon dioxide; OMP decarboxylase; OMPDCase;
                     OMPdecase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HEY0294075.1"
                     /translation="AGMMINSSRAIIYASKGEDWREAAAAAAKGLRDAINAVR"
     gene            complement(139..669)
                     /locus_tag="VGC69_01940"
     CDS             complement(139..669)
                     /locus_tag="VGC69_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEY0294076.1"
                     /translation="MITPTALVLPIIQGLAEQLGAVLLDRGLMMGTAESCTGGLLAGA
                     ITSVAGSSGWFERGFVTYTNDAKVQDLDVSEDVLAHYGAVSEPVALEMAGGVLLAVER
                     AQVAVSTTGIAGPGGATAGKPVGMVCFGFALRTAEGIATRAATHIFPGDRAQVRHGAV
                     EFALRGVLEMLVQPSH"
     gene            complement(666..1175)
                     /locus_tag="VGC69_01945"
     CDS             complement(666..1175)
                     /locus_tag="VGC69_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY0294077.1"
                     /translation="MRERAKVAFPSWRWVCSRLYRLVAFGFCSGLLRPGSGTWGTLLA
                     WPLWLLLSPWVPSDRVMGVLLLALFVYGCWACTRAGNELGEPDHVGMVWDEIVAFWIV
                     LWLVPPSLGSQALAFVLFRFFDTVKPQPIRYFDARLKGGFGVMWDDILAAAYTLLVMA
                     LAVRFGVIS"
     gene            complement(1300..2259)
                     /gene="thiL"
                     /locus_tag="VGC69_01950"
     CDS             complement(1300..2259)
                     /gene="thiL"
                     /locus_tag="VGC69_01950"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY0294078.1"
                     /translation="MASEFDLIAKHFTRPAAPGQLGVGDDCALFSVPPGHQVATSTDL
                     LVEGRHFYPDVDPQALGHKTLAVNLSDLAAMGAQPIGCLLGLALPQADEAWLAAFARG
                     FHDLAGEAGCPLIGGDTTRSPKLLTLSVTVFGSVAPDKALRRGAARAGDEIWVSGELG
                     AADVAYRLLEGQLPADPRRLAAVRRAMEWPVPRLALGRALAGLAHAAIDISDGLLQDL
                     GHILDLSRVGAELRYEALPVAPALAGLDEAVRRRAVLGGGDVYELCFTADPADQARVL
                     HAAAAAGVRVSRVGRITADAGLIVLDGRGQPLPDLPQGFDHFA"
     gene            complement(2396..2899)
                     /gene="nusB"
                     /locus_tag="VGC69_01955"
     CDS             complement(2396..2899)
                     /gene="nusB"
                     /locus_tag="VGC69_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0294079.1"
                     /translation="MTTKKTSAETAAQARSNARSARHRAREFALQGVYAWLLRGGEGT
                     QEAGEIDAHLRDDEDFSEADAQWFKTLLHGVLQDAPGLRERFTPYVDRPLAELSPVEH
                     GILLIGSYELVHHVEVPYKVAINEAVELAKSFGGTDGFKFVNGVLDKLAADVRSVEVQ
                     AAAQQRR"
     gene            complement(2874..3383)
                     /gene="ribH"
                     /locus_tag="VGC69_01960"
     CDS             complement(2874..3383)
                     /gene="ribH"
                     /locus_tag="VGC69_01960"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY0294080.1"
                     /translation="MNPYILTPDLNGEGLHIGIVRARFNEEIGQAELDACLEELGKLG
                     VDERDIMIATVPGALELGVALSHMAETFEFDALIALGAVIRGETYHFEVVSNEMATAV
                     SRISLETGIPVANGVLTVDTDEQAQARAAGKGRDCAQVAVEMANLVAALEPEEEDEDE
                     DFDDEEDER"
     gene            complement(3397..4563)
                     /gene="ribBA"
                     /locus_tag="VGC69_01965"
     CDS             complement(3397..4563)
                     /gene="ribBA"
                     /locus_tag="VGC69_01965"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY0294081.1"
                     /translation="MSVHPLNAAGAAADDFQIAPVPEIIAELRSGRIVILVDEEDREN
                     EGDLVMAAEFVTPEAINFMVTHGRGLVCLTLTEERCRQLDLPMMTSRNGTRYGTNFTQ
                     SIEAAEGVETGISAADRARTIQVAVARNTKPSDLVQPGHIFPVRAVPGGVLVRAGHTE
                     AGCDLTRMAGLTPAAVICEILKPDGTMARLPDLVAFAREHGLKIGTIASLIQYRSEHE
                     SIIQRVGERPMQTPWGEFRAVAYRDDASGSPHVALVHGGIDPQRETLVRVHEPASVLD
                     VLDVGHGAHSWTVAQALTAIAASPAGVLVLMNCQAPTEHIFGQIGRWADPTAVPPPSD
                     GERFGLRTYGIGAQILRDLNVGRMRLLARERKMPSMAGFSLSITGYDFPPSVNK"
     gene            4787..6451
                     /locus_tag="VGC69_01970"
     CDS             4787..6451
                     /locus_tag="VGC69_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEY0294082.1"
                     /translation="MSEQELKLHVPAAAGATIAKELLKRGATRIRLHALYFDTPDREL
                     ARARIALRLRLEGRQWMQTLKTPGADAITRQELNHPRPGPVLDLSVYAGTELEAALSK
                     LRGPLGLRYETDVQRLLCKVRSRQGTVELAYDTGLLRAGTLELPISELEFELMSGRPA
                     AIFAVSHGWQKRHQLLLDTRSKSHRGDALATLAPQLGAAESDKARANLIAGFWAPRGA
                     SGVVLDPAMDAPAAMAAIAAECIEQIACNAAALAEVDTLGVYQAGNSEHVHQLRVGMR
                     RLRSAWRLFNGWIAPPPAALLEGMRAHFAAFGANRDQDVLEETVAPMLQKAGMPVIPA
                     QPRPGEQADAHAIAAGPAFQTWLVDLLEWSLNVQPPAQPAEMPGAAVGDEPGADAPEG
                     TAQAGAATDAAEAEPPSGPIEPIIIPLHQARPPAPLPKRLANRLHRWHERVAGQGLHF
                     GALTLEDRHELRKRGKRLRYGLAFAESLLPAARLQPYRKQLSLVQDLLGEINDLAVAR
                     DHYRAQCEVHPQAWFALGWIEARLDALVAQAQQAFTDLANTKPFWK"
     gene            complement(6524..7600)
                     /gene="ftsY"
                     /locus_tag="VGC69_01975"
     CDS             complement(6524..7600)
                     /gene="ftsY"
                     /locus_tag="VGC69_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY0294083.1"
                     /translation="MFSFFKKKPAPAEIPSVSAQPEAAEPAPAPVPEPAPEPIAEPVV
                     EPAVETPAAAPKASWLSRLRQGLSRTGQSLGSLFVGVKVDESLFEDLESALIMADAGV
                     EATQKLLDVLRARVKKDRIEDAAQVKVALRELLAEHLRPLEKTFDLKRAQPLVVMIAG
                     VNGAGKTTSIGKLAHDFQRQGASVLLAAGDTFRAAAREQLVEWGSRNNVAVIAQDGGD
                     PAAVAFDAVNAGRARGTGVVMVDTAGRLPTQLHLMEELKKIRRVIGKADAAAPHEVLL
                     VIDGNTGQNALAQIRAFDAAVNLTGLVVTKLDGTAKGGTLAAVAAGSQGVRPIPVYWI
                     GVGESMEDLQPFVAAEFAAALLAD"
     gene            7640..8212
                     /gene="rsmD"
                     /locus_tag="VGC69_01980"
     CDS             7640..8212
                     /gene="rsmD"
                     /locus_tag="VGC69_01980"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY0294084.1"
                     /translation="MASSNIRIVAGQYRRTPIPVPDVPGLRPTPDRVRETLFNWLAHL
                     WDGDFADKRVLDLFAGSGALGFEAASRGAAQVQMVERDRHAVAALRALRDKLHANAVR
                     IHAGDSLEALQRMDASRYDLVLLDPPFNQGWLPRLWPLLPAVLPENGPGLVYVEAEAP
                     CEAPDGFELLRDAKAGAVHYHLFRIAALQN"
     gene            8273..8779
                     /gene="coaD"
                     /locus_tag="VGC69_01985"
     CDS             8273..8779
                     /gene="coaD"
                     /locus_tag="VGC69_01985"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY0294085.1"
                     /translation="MITAVYPGTFDPLTRGHEDLVRRAAGLFDKVVVGIAHSRNKKPF
                     FNIDERVEIAKEVLGHYPNVDVKSFGGLLKDFVREQGATVIVRGLRAVSDFEYEFQMA
                     GMNRHLLPDVETLFMTPSDQYQFISGTIVREIAQLGGDVSKFVFPSVERWLQAKSKEH
                     REQSWPDA"
     gene            complement(8797..9186)
                     /locus_tag="VGC69_01990"
     CDS             complement(8797..9186)
                     /locus_tag="VGC69_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY0294086.1"
                     /translation="MKMSGFQPVAECGLTQQAEAAAYQRRWLVTNDAGQWLNRELFPK
                     LAEITVELRLGYLVLKAPGMLRMDIPLDVIEDDDSVRYQISLGGQAVDVVDEGQLAAA
                     WMSNFIGIPCRLFKVHPDMGDVHWPAA"
     gene            9284..10078
                     /gene="otnI"
                     /locus_tag="VGC69_01995"
     CDS             9284..10078
                     /gene="otnI"
                     /locus_tag="VGC69_01995"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEY0294087.1"
                     /translation="MSLKFAANLSFLYQDLPFLDRIGAAARDGFEGVEYLFPYDFPAQ
                     EIRRRLVDAGIDQALFNAPAGDWAQGERGTASLPGRTAEFRTGIMRALDYASVLGNTR
                     LHVMAGLLPQSADRAVHLAAYQSHVAWAAEQAARQGVAIMLEPINQRDMPGYFLSYQQ
                     DALAVIDAIGAPNLRVQFDCYHCQIMEGDIAMKLRAMFDRIGHVQVASVPGRNEPDGQ
                     ELHYPYIFRTLDELGYAGWVGCEYKPRADTRAGLGWLRAYRQLAGQ"
BASE COUNT         1638 a         3554 c         3371 g         1526 t
ORIGIN      
        1 cgcgggcatg atgatcaact cctcgcgcgc catcatctac gccagcaagg gcgaagactg
       61 gcgcgaggcg gccgcggcgg cggccaaggg attgcgcgac gcgatcaacg cggtgcgctg
      121 attcggcggc ctaatggcct aatgcgacgg ctggaccaac atttccagca cgccgcgcag
      181 ggcgaactcc acggcgccat ggcgcacctg ggcgcggtcg cccgggaaga tgtgcgtggc
      241 ggcgcgcgtg gcaatgccct cggccgtgcg cagcgcgaaa ccgaagcaca ccatgcctac
      301 cggcttgccc gccgtggcgc cgcccggccc ggcaatgccg gtggtggaca ccgccacctg
      361 ggcccgctcg acggccagca gcaccccgcc ggccatttcc agggccaccg gctcgctcac
      421 cgcgccgtaa tgcgccagca cgtcctcgga gacgtccaga tcctgcacct tggcgtcgtt
      481 ggtgtaggtg acgaagccgc gctcgaacca gccgctggaa ccggccaccg aggtgatggc
      541 gcccgccagc aggccgccgg tgcaggattc ggccgtgccc atcatcaggc cacggtcgag
      601 caggacggcg cccagctgtt cggccaagcc ttgtatgatg ggaaggacga gtgccgtggg
      661 tgtaatcatg agataactcc gaaacgcacg gccagcgcca tcaccagcag ggtataggcc
      721 gccgcgagaa tatcgtccca catcacgccg aaaccgccct tgagacgcgc atcgaagtac
      781 cggatgggct gtggcttgac ggtatcgaag aaacgaaaca gcacaaaggc cagggcttgc
      841 gagccgagcg acggcggcac cagccacagc acgatccaga aggccacgat ttcgtcccag
      901 accatgccca cgtggtcggg ctcgccgagt tcgttgccgg cccgcgtgca ggcccagcag
      961 ccgtagacga acagcgccag caacagcacg cccatgaccc ggtcggacgg cacccagggc
     1021 gagagcagca gccacagcgg ccacgccagc aaggtgcccc aggtgcccga gccgggccgc
     1081 aacaggccgc tgcagaaacc aaaggctacc aggcggtaga ggcgcgaaca cacccagcgc
     1141 caggagggaa aggccacctt cgcgcgttcg cgcatcgagg gcgagaccgg tgcggcgggg
     1201 acgggagtcg ggtcgggctg ttggtcgggc atgagaggca tgaagggcgc gcacgatgcg
     1261 cgcgggttcg acggctaggc tcacgcggct gcgcgggcct caggcaaaat gatcgaagcc
     1321 ttgcggcaag tctggcaagg gctggcccct gccgtcaagc acgatcaggc ccgcatcggc
     1381 cgtgatgcgg ccgacgcggc tcacgcgcac gcccgccgcg gccgccgcgt gcagcacgcg
     1441 cgcctggtcg gccgggtcgg cggtaaagca cagctcgtag acgtcgccgc cgcccagcac
     1501 ggcacggcgg cgcacggctt cgtccaggcc tgcgagcgcg ggcgccacgg gcaatgcctc
     1561 gtaccgcagc tcggccccga cccggctgag atcgaggatg tggcccaggt cctgcagcaa
     1621 cccgtcggaa atatcgatgg cggcatgggc caggccggcc agggcgcggc ccagggccag
     1681 cctgggcacg ggccattcca tcgcgcggcg cacggcagcc aggcgccgcg ggtcggccgg
     1741 cagctggcct tccagcagcc gataggccac gtcggcggcg cccagttcgc cggaaaccca
     1801 gatttcgtcg cccgcgcgcg cggcgccgcg ccgcagggcc ttgtccggcg ccacggaacc
     1861 gaacacggta acgctgaggg tgagcagctt gggactgcgc gtggtgtcgc cgccgatgag
     1921 cgggcagccg gcctcgcccg ccaggtcatg gaagccgcgt gcgaaggcgg ccagccaggc
     1981 ttcgtcggct tgcggcaagg ccaggcccag caggcagccg atgggttgcg cgcccatggc
     2041 ggccaagtcg gacagattga cggcgagcgt cttgtggccg agggcctgcg ggtcgacgtc
     2101 ggggtagaag tgccggcctt cgaccagcag gtcggtgctg gtggctacct ggtggccggg
     2161 cgggacggag aacagggcgc agtcgtcgcc cacgcccagt tggccggggg ccgcggggcg
     2221 ggtgaagtgc ttggcgatga ggtcgaactc ggaggccatg ggggatctta tggaccggtg
     2281 ggttgagttc ttgaggcgcc gaggctgccc tgcggaattc cgatgctcgg cgctggtagc
     2341 gcctgtgctt cggtttcctc cggggtcctc ggctcaaggc atcatcgtgc cgcgatcaac
     2401 gccgctgctg cgcggcggcc tgtacttcca ccgagcggac atcggcggcc agcttgtcga
     2461 gcacgccgtt gacgaacttg aagccatcgg tgccgccgaa ggacttggcc agttccacgg
     2521 cctcgttgat ggcgaccttg tagggcactt cgacgtggtg caccagctcg tagctgccga
     2581 tcagcaggat gccatgctcc accggcgaca gctcggccag cgggcggtcg acgtagggcg
     2641 tgaagcgctc gcgcaggccg ggcgcgtcct gcagcacgcc gtgcagcagc gtcttgaacc
     2701 actgcgcgtc ggcctcggag aagtcctcgt cgtcgcgcag gtgcgcgtcg atctcgccgg
     2761 cttcctgggt gccttcgccg ccgcgcagca gccacgcgta cacgccctgc agcgcgaact
     2821 cgcgcgcgcg gtggcgggcg ctgcgggcgt tgctgcgggc ttgcgccgcg gtctcagcgc
     2881 tcgtcttctt cgtcgtcaaa atcttcgtcc tcgtcttctt cttcgggttc gagcgcggcg
     2941 accaggttgg ccatttccac ggccacctgg gcgcagtcgc ggcccttgcc cgcggcgcgc
     3001 gcctgggctt gttcgtcggt atcgaccgtc agcacgccgt tggccacggg aatgcccgtt
     3061 tccagcgaga tacggctgac cgccgtggcc atttcattgc tgaccacttc gaaatggtag
     3121 gtttcgccgc gaatcaccgc gcccagcgcg atgagggcgt cgaactcgaa ggtctcggcc
     3181 atgtgcgaga gggccacgcc cagttcgagc gcgccgggca cggtggcgat catgatgtcg
     3241 cgttcatcca cgcccagctt gcccagttct tcgaggcagg catccagctc ggcctggccg
     3301 atctcttcgt tgaagcgggc acggacgatg ccgatgtgca ggccttcgcc gttcagatcc
     3361 ggggtaagga tgtagggatt catagctagg ccttgtttat ttgtttacgg aaggagggaa
     3421 atcgtaaccc gtaatgctca gagaaaagcc cgccatgctg ggcatcttgc gttcacgcgc
     3481 cagcaggcgc atgcggccga cgttcaagtc gcgcaggatc tgcgcgccga tgccataggt
     3541 gcgcaggccg aaacgctcgc cgtcggaggg cggcggcacg gccgtgggat cggcccagcg
     3601 gccgatctgc ccgaagatgt gctcggtggg cgcctggcag ttcatcagca ccagcacgcc
     3661 ggccggcgac gcggcgatgg ccgtcaacgc ctgggccacc gtccagctgt gcgcgccatg
     3721 gcccacgtcc agcacgtcga gcaccgaggc gggctcgtgc acgcgcacca gggtttcgcg
     3781 ctgagggtcg atgccgccgt gcaccagcgc cacgtgcggc gagccggagg cgtcgtcgcg
     3841 ataggccacg gcgcggaact cgccccaggg cgtctgcatg gggcgctcgc ccacgcgctg
     3901 gatgatggac tcgtgttcgc tgcggtactg gatgagcgag gcgatggtgc cgatcttcag
     3961 gccgtgctcg cgcgcgaagg ccaccaggtc gggcaggcgg gccatggtgc cgtcgggctt
     4021 gaggatctcg cagatcacgg ccgccggcgt caggcccgcc atgcgggtca ggtcgcagcc
     4081 ggcctcggtg tggccggcgc gcaccagcac gccgccgggc accgcgcgca ccgggaagat
     4141 gtggccgggc tgcaccaggt cggacggctt ggtgttgcgc gccacggcca cctggatggt
     4201 gcgggcgcgg tcggcggccg agatgccggt ttcgacgcct tcggccgctt cgatggactg
     4261 cgtgaagttg gtgccgtagc gcgtgccgtt gcggctggtc atcatgggca ggtcgagctg
     4321 gcggcaacgc tcttcggtga gcgtcaggca caccaggccg cggccgtgcg tgaccatgaa
     4381 attgatggcc tcgggcgtga cgaactcggc ggccatgacc aggtcgcctt cgttttcacg
     4441 gtcttcttcg tcgaccagga tgacgatgcg cccggagcgc agttcggcga tgatctcggg
     4501 taccggcgcg atctggaaat cgtcggcggc ggcgccggcg gcattgaggg gatggacaga
     4561 catggatgtg gcctgcggga gaggcaaaaa agccatttta ccgccccccg gccggctacc
     4621 gggggtaagc ccggatcgga tggcgagata tcgctttacc cgccccgggc caggggcaac
     4681 aatcgaaggc ggagaacgaa gatgaccaat ctatgactag tcatcataaa tcgtggctct
     4741 ctcctataag atccctgcct ttcccggctg tgagtcccta cggcacatgt cagaacaaga
     4801 actgaaacta cacgtgccgg cggccgccgg cgccacgatc gcaaaagaac tcctcaagcg
     4861 cggcgccacc cgcatcaggc tgcacgccct gtatttcgac acgcccgacc gcgaactggc
     4921 gcgcgcgcgc atcgcgctgc gcctgcgact ggaaggccgc cagtggatgc agacgctcaa
     4981 gacgcccggc gccgacgcca tcacgcggca ggagctcaac catccgcgcc cgggccccgt
     5041 gctcgacctg tcggtctacg ccggcaccga actcgaagcc gcgctgtcca agctgcgcgg
     5101 ccccctgggc ctgcgctatg aaaccgacgt gcagcgcctg ctgtgcaagg tccgcagccg
     5161 ccagggcacg gtcgagctgg cctacgacac gggcctcttg cgcgccggca cgctggagct
     5221 gccgatctcc gagctggaat tcgaactgat gtcgggcagg cccgccgcca tcttcgcggt
     5281 gtcccatggc tggcagaagc gccaccagtt gctgctggac acgcgcagca agtcccatcg
     5341 cggcgacgcc ctggcgacgc tggcgccgca actgggcgcg gccgagtccg acaaggctcg
     5401 cgccaacctg atagccggct tctgggcgcc gcgcggcgca tcgggcgtcg tgctcgatcc
     5461 cgccatggac gcgcccgccg ccatggccgc catcgcggcc gaatgcatcg agcagatcgc
     5521 ctgcaacgcc gccgcgctgg ccgaggtgga cacgctgggc gtctaccagg ccggcaactc
     5581 ggaacacgtg catcagctgc gcgtgggcat gcgccgcctg cgttcggcct ggcgcctgtt
     5641 caacggctgg atcgccccgc ccccggccgc gctgctcgag ggcatgcgcg cgcacttcgc
     5701 cgccttcggc gccaaccgcg accaggatgt gctcgaagaa accgtggctc ccatgctgca
     5761 gaaggccggc atgcccgtca ttccggccca gcctcgcccg ggcgaacagg ccgacgccca
     5821 tgccatcgcc gccggcccgg ccttccagac ctggctggtg gacttgctgg aatggagcct
     5881 gaacgtgcag ccgccggccc agcccgccga aatgccgggt gcggccgtcg gcgacgaacc
     5941 cggcgcggat gcgccggaag gcaccgccca ggccggcgcc gcgacggacg cggccgaagc
     6001 cgagccgccg tcgggcccca tcgaacccat catcatcccc ctgcaccagg cccgcccgcc
     6061 cgcgccgctg cccaagcgcc tggccaaccg gctgcaccgc tggcacgaga gagtggccgg
     6121 gcaaggcctg cacttcggcg cgctcaccct ggaagaccgc cacgaactgc gcaagcgcgg
     6181 caagcgcctg cgctacggcc tggccttcgc cgaatcgctg ttgccggccg cccggctgca
     6241 accctatcgc aagcagctgt cgctggtgca ggacctgctc ggcgagatca acgacctggc
     6301 cgtggcccgc gaccactacc gggcgcaatg cgaagtccat ccgcaggcct ggttcgcgct
     6361 gggctggatc gaagcgcgcc tggacgccct ggtcgcccag gcccagcagg ccttcaccga
     6421 cctggccaat accaagccgt tctggaagta accggattct ggttgagccg ccgctgagct
     6481 tgagttcttg aggcgccgag gctgccctgc ggaattccga tgttcaatcc gccagcagcg
     6541 cggcggcgaa ctccgccgcc acgaagggct gcaggtcttc catgctctcg cccacgccga
     6601 tccagtagac cgggatcggc cgcacgccct ggctgccggc cgccacggcg gccagcgtgc
     6661 cgcccttggc cgtgccgtcg agcttggtca ccaccaggcc ggtgaggttg acggccgcat
     6721 cgaatgcgcg gatctgcgcc agcgcgttct ggccggtatt gccgtcgatc accagcagca
     6781 cctcgtgcgg cgcggccgca tcggctttgc cgatcacgcg gcggatcttc ttgagttctt
     6841 ccatcaggtg cagctgcgtg ggcaggcggc cggccgtgtc caccatcacc acgcccgtgc
     6901 cgcgcgcgcg gccggcattg acggcgtcga aggccaccgc ggccgggtcg ccgccgtcct
     6961 gcgcgatcac cgccacattg ttgcggctgc cccactccac cagttgctcg cgcgccgcgg
     7021 cgcggaaagt atcgccggcc gccagcagca cgctggcccc ctggcgctgg aaatcatgcg
     7081 ccagcttgcc gatggacgtg gtcttgcccg cgccgttcac gcccgcgatc atcaccacca
     7141 gcggctgcgc gcgcttgaga tcaaaggtct tttccagcgg ccgcaaatgc tcggccagca
     7201 gttcgcgcaa ggccaccttc acctgcgcgg cgtcttcgat gcggtctttc ttgacgcggg
     7261 cgcgcagcac gtccagcagt ttctgcgtgg cctcgacgcc cgcgtcggcc atgatgagcg
     7321 ccgactccag atcctcgaac aggctctcgt cgaccttgac gcccacgaag aggctgccca
     7381 ggctctgccc ggtacgcgac aagccctgcc tgagccggct cagccacgag gccttgggcg
     7441 cggcggcggg cgtctcaacc gcgggctcga ccacaggctc ggcaataggc tcgggagccg
     7501 gctccggcac gggggcaggc gccggctcgg ccgcctcggg ctgggcagaa acggaaggga
     7561 tctcggcggg cgccggcttt ttcttgaaga agctgaacat gggcgaaagt ataaagcggc
     7621 ggccccctac aatccccgca tggccagttc caatatccgc atcgtcgccg gacaataccg
     7681 gcgcaccccc atccccgtcc ccgacgtgcc gggcctgcgc cccacgcccg accgcgtgcg
     7741 cgaaaccctc ttcaactggc tcgcccacct gtgggacggc gacttcgccg acaagcgcgt
     7801 gctggacctt ttcgccggca gcggcgccct gggcttcgaa gccgcctcgc gcggcgccgc
     7861 ccaggtgcag atggtcgaac gcgaccggca cgccgtcgcc gccctgcgcg cgctgcgcga
     7921 caagctccac gccaacgccg tgcgcatcca tgccggcgac tcgctcgagg ccctgcagcg
     7981 catggacgcc tcccgctacg acctcgtcct gctcgacccg cccttcaacc agggctggct
     8041 gcccagattg tggcccctgc tgcccgccgt gctgcccgaa aacggcccgg gcctggtcta
     8101 tgtcgaagcc gaggcgccct gcgaggcgcc cgacggattc gaattgttac gagatgctaa
     8161 agccggcgcg gtgcattatc atctgttccg aattgctgcg ttgcaaaatt aagtcaataa
     8221 tccggggttc gtgagatggt gcaaaccatc cgccatgcga ggagccaccc atatgatcac
     8281 tgccgtctac ccgggcacct tcgacccgct cacccgcggc cacgaagacc tggtgcgccg
     8341 cgccgcaggc ctgttcgaca aagtcgtcgt cggcattgcg cacagccgca acaaaaagcc
     8401 tttcttcaat atcgacgagc gcgtcgagat tgccaaggaa gtcctgggcc actaccccaa
     8461 cgtcgacgtc aagagcttcg gcggcctgct caaggacttc gtgcgcgagc aaggcgccac
     8521 cgtcatcgtg cgcggcctgc gcgccgtgtc cgacttcgag tatgaattcc agatggccgg
     8581 catgaaccgc cacctgctgc ccgacgtcga aaccctcttc atgacgccct cggaccaata
     8641 ccagttcatc tcgggcacca tcgtgcgcga gatcgcccaa ctgggcggcg acgtcagcaa
     8701 gttcgtgttt ccctcggtgg agcgatggct gcaggccaag tccaaggagc accgcgagca
     8761 gagctggccc gacgcctgac gcgggcccgc tggccgctac gcggcaggcc agtgcacgtc
     8821 gcccatatcg gggtgcacct tgaacaggcg gcaggggatg ccgatgaaat tcgacatcca
     8881 ggccgccgcc agctggccct cgtcgaccac gtccaccgcc tgcccgccca gcgagatctg
     8941 gtagcgcacg ctgtcgtcgt cctcgatcac gtccagaggg atgtccatgc gcagcatgcc
     9001 cggcgccttg agcaccaagt agcccaggcg cagctccacc gtgatctcgg ccagcttggg
     9061 aaacagctcg cggttgagcc actgcccggc gtcgttggtc accagccagc gcctttggta
     9121 ggcggccgcc tcggcctgct gggtcaggcc gcattcggcc acgggctgga atccgctcat
     9181 cttcactccg gtctggctgt tttctggggt tgtcatgccc gcatggtagc aaagcccgac
     9241 cacgcaatgc actgaatacc cgccatcgat cccggagccg gatatgtcct tgaagttcgc
     9301 cgccaacctg agttttctat accaggacct gcctttcctg gatcgcatcg gcgccgccgc
     9361 gcgcgatgga ttcgagggcg tcgaatacct cttcccctac gactttcccg cgcaggagat
     9421 caggcggcgc ctggtcgatg ccggcatcga ccaggccctg ttcaacgccc ccgcgggcga
     9481 ctgggcgcaa ggcgagcgcg gcacggcctc gctgcccggc cgcaccgccg aattccgcac
     9541 cggcatcatg cgggccctgg actatgccag cgtgctgggc aacacccgcc tgcacgtcat
     9601 ggccggcctg ctgccccaaa gcgccgaccg cgccgtccac ctggccgcct accagtccca
     9661 cgtggcctgg gccgcggaac aggcggccag gcaaggcgtc gccatcatgc tcgagcccat
     9721 caaccagcgc gacatgcccg gctatttcct gagctatcag caagatgccc tggccgtcat
     9781 cgacgccatc ggcgcgccga acctgcgggt gcagttcgac tgctatcact gccagatcat
     9841 ggaaggcgac atcgccatga agctgcgcgc catgttcgac cgcatcggcc acgtgcaggt
     9901 cgccagcgtg cccggccgca acgaacccga cggacaggaa ctccactacc cctacatttt
     9961 ccggacgctg gacgaactgg gctatgccgg ctgggtcggc tgcgaataca agccgcgcgc
    10021 cgatacccgg gccggactgg gctggctgcg cgcgtatcgg caattggccg gccaatgaaa
    10081 aagccatgc
//
LOCUS       DASWPO010000038        14082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1525474, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000038 DASWPO010000000
VERSION     DASWPO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14082
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525474"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VGC69_02000"
     CDS             complement(<1..545)
                     /locus_tag="VGC69_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294088.1"
                     /translation="MNRDVELDGRRVVQVDGLTVRFSTPSRVVEAVSDVSFHVDRGET
                     LAIVGESGSGKSVTSLSLMRLIEYGGGKIAGGDMRLRRRNGELLDLARADESALRRVR
                     GADIAMIFQEPMTSLNPVFTAGTQIAEALQLHQGLDAGAARAEALRMLERVRIPEARA
                     ILDRYPHQLSGGMRQRVMIAMA"
     gene            complement(550..1422)
                     /gene="nikC"
                     /locus_tag="VGC69_02005"
     CDS             complement(550..1422)
                     /gene="nikC"
                     /locus_tag="VGC69_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HEY0294089.1"
                     /translation="MSTPTVTAIAVPQRSGSRAWAKFRRNRTAMLGAAIVLVFVALAI
                     LASVLANADPLKTSFMTVRKAPSHLFWLGTDELGRDIYSRMLFGARASLMVGLVSVVI
                     ALVVGVPFGLLAGYFGGWVDSVISRVTEALLAIPFLILAIALAAFLGPSLVNAMIAIG
                     VSAAPKFTRLTRGQVLAVKNEDYVQGARALGASDLRIIVRHVLPNVMPPIIVQATITI
                     ATSIIAEASLSFLGLGLQPPNPSWGSMLNTAKNFMSQAPWMSIFPGAAIFLVVLGFNL
                     LGDGLRDALDPRQE"
     gene            complement(1419..2363)
                     /locus_tag="VGC69_02010"
     CDS             complement(1419..2363)
                     /locus_tag="VGC69_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294090.1"
                     /translation="MLRLILRRVIVSIPTLILVSMIVFMLQKILPGDPVLAMAGEDRD
                     PAVLDYLRGKYHLNDPLPVQYGAWLMQVAHGDLGKSLRTDVPVNELIRQKLPVTTQLS
                     VMAMIFALLLGIPAGIIAAVRKGSAVEMGANMAALSGMSIPNFWLGIILILVVSVEWQ
                     LLPASGYVPLTEDFWLSIKTLLMPSFVLSLAIAAYLMRHTRSAMLEALGADYVRTARA
                     KGVSPYKVVMRHALRNALMPIVTLVTLLFGELLAGAVLTEQVFTIPGFGKLIVDAVFN
                     RDYAVVQGVVLCVAVGFILLNLLADVLYIVVNPRLRHS"
     gene            complement(2427..3932)
                     /locus_tag="VGC69_02015"
     CDS             complement(2427..3932)
                     /locus_tag="VGC69_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294091.1"
                     /translation="MGKVLGGVLLALASATALAQELRIGLQEDPDTLDPHRARTYVSR
                     IVFTSLCDKLVDINDKLEFVPQLAESWSWSPDDKVLTFKLRSDVVFHDGTKFDAAAAK
                     ANIDREMTMPESMRKSEITSVASVAAPDPTTLVLTLKQPDATLLAALSDRAGMMLSPA
                     SFTGKEVGAVARSPVCSGPYKFVERVQNDRIVLDKFDKYYDAKDYHFDRVVFLPIPDT
                     TVRLSNLRSGGLQILERLGPSDVPQVKQDASLVFAPVNGLGYQEVYFNVGNGPVSKTS
                     PFKDPRVRKALELSIDRNAINEVVGNGIYPPAAQPFPPSSPYFSDKFQPPARDLDKAK
                     ALLKEAGVTLPVKGELSFGNDTTITAMAQMIQAMSAEAGFDLSLRPTEYAALLSQAQQ
                     GNFELSLRGWSGRVDPDGNIQAFVTCKGPQNDGHYCNAEVDRLLEEARHVPDVAQRKA
                     LYEQAETILHNDEENFFLYFQPWPFALQKKVVGFKPSPDGMIRLKGVSFAK"
     gene            complement(3987..5615)
                     /locus_tag="VGC69_02020"
     CDS             complement(3987..5615)
                     /locus_tag="VGC69_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY0294092.1"
                     /translation="MKSFNWTNPYTSHRTPVFARNVVSTSHPLAAQAGLRMLLKGGSA
                     VDAAVAAAAVITLTEPVSCGLGSDCFAILWDGKALHGLNSSGVAPAAWSVDYFKKKYG
                     TGADGLAVQPKRGWDAVTVPGVVAGWAALHEKFGKLPFETLFEPAIEIAERGYAVPPM
                     VAHKWKLAADELHSQPGYAEAFMPNGRAPRVGEHFRLPEAAYTLRRIAESKGRDYYEG
                     DIAERIAAFSKECGGAMTLDDLRNYRPMWVKPISQSYRGYEMHEIPPNGQGIAALIAL
                     GICEHFDLKGMGVDTLQSQHVQIEAMKLAFTDIYKYVADPRSMEVTPEQMLDPAYLAS
                     RAKLIDLGKAAHPTSGRPAAGGTIYMSAADESGMMISFIQSNYMGFGSGIVVPGTGIS
                     LQNRGVGFSMDPVSANVVEGGKRPFHTIIPGFLTQGGRPVMSFGVMGGDMQPQGHLQT
                     AIRMIDYDQQPQAACDAPRWKLNRDQTLDVEPAMDRQVVEGLAQMGHKLKSVDDPYMD
                     FGAGQFIWRMSPDDNEQGYVAASDPRRDGLAAGF"
     gene            5784..6689
                     /locus_tag="VGC69_02025"
     CDS             5784..6689
                     /locus_tag="VGC69_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294093.1"
                     /translation="MSTLRFLRTFLAVVHHGSFSEAAEHVALTQAAVSFQMRALENEL
                     GRELFDRSGRLALLSAAGRELVPETMRLLELYDRMRLPPAASDTLAGSVSVGAIVSCM
                     GTLSKVVSAMKREHPALDVRIFSGKSSELAGKVEAGELDAAFLVEAGRPTASTRWITL
                     YEEPLVVVAPASAPGKTVREVLAQSPFLRFDRTQRTGRQIDRVLRRLGVSIHEFLELN
                     AIETLVELVRQEVGVTLLPLLNGVNWQQTPDLRVLSLPSDLGPFTRAIGMLERRDPAR
                     QAITTAICALSGAAFRKRMAGAATA"
     gene            complement(6773..8563)
                     /locus_tag="VGC69_02030"
     CDS             complement(6773..8563)
                     /locus_tag="VGC69_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294094.1"
                     /translation="MDSHVYIDDFFVTGETDYSAALMRAILHLRPFGGGVIEYGSGTF
                     VHAPYASIIAPDEGTAATVSSLDPNISIVGQGSGATIWVPTSNKLEFIAQRGANLRIG
                     GIWVYGDEYGLLQDVPKQPLITPSSGLAGLGNNAFSFIRQYDGNGGLVIDDVTCSQIN
                     VGVHYCGDYTDEETLYGDLRTRDYYTDTCCFGLLVEQPERISIIHGGSKNTKISTNAD
                     GSTDPGHYLYVTNRIGAAPHTINISHISGYKDHNSTLKVRKGEVVVISNVALNQCGRG
                     IDIVDVQSGSLSNYAAVCLQDALNNPTGVGTNMLEITDTSNFVVDGVEIDIRGQMAYG
                     ILMRTDTNAANAKNVTVNNVIVVGDYTDTGKQPLLCVGQSDIKIRDFQFVHTGSTPSP
                     DRWPIAISGCSNVHVINPAHYVDDPSLSDASYLVGFDATCVACKVTYSDGHISVDATT
                     NTVHVSTGGDVTVFNESRAFRLGYDRSTQLDPASTLIAGVQIFSSGQQITSTSGIAHV
                     WQRIGGDGTLLRAFNQNTIVATMATVGSRAQLSLDGSTQCFDTTGAGSPEGVITAGVG
                     STYRRRDGGAGTSFYVKESGGYSNTGWVAK"
     gene            complement(8759..9097)
                     /locus_tag="VGC69_02035"
     CDS             complement(8759..9097)
                     /locus_tag="VGC69_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY0294095.1"
                     /translation="MKQVTAIIKPFKLDEVREALAEAGVSGLTVVEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIRVEVVLPDDRVETAIEAVLKAARTGKIGDGKIFVTPVEQAIRI
                     RTGESDDDAL"
     gene            complement(9144..10778)
                     /locus_tag="VGC69_02040"
     CDS             complement(9144..10778)
                     /locus_tag="VGC69_02040"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY0294096.1"
                     /translation="MSAARVAFAQINTVVGGLASNAAKILQAAREAHAQGADVLVTPE
                     LSLTGYPPEDLLLRPSFIDAQARALQQLCADLAGLAGLHVVVGHAARREGRPHNAASV
                     LCEGRVLGTYAKRELPNYSVFDEKRYFEPGESSLVFEVKGVRFGVNICEDIWFDSAPR
                     AAAAAGARVLLVPNASPYNQGKQEERVEVARRSLQGTGCALVYANLVGGQDELVFDGL
                     SFVLDAEGRQMAQLPGFTEDLGLVEIDAAGSVRALDGLPEAFETSQEEEVWNALVLGV
                     RDYLGKNGFPGAIIGLSGGIDSAVVLAVAVDALGADNVRAVMMPSRYTADISQLDASD
                     MARRLGVRYDEIAIAPAFQAFESMLAGQFAGLPVDATEENIQARVRGTLLMALSNKTG
                     RLVLTTGNKSEMTTGYCTLYGDMAGGFAVIKDVPKTLVYRLAVWRNTRGEVIPERIIT
                     RPPSAELRPDQTDQDSLPPYEVLDGIMARYMEHNESPAQIVAAGYPAAAVEQVLRLIR
                     INEYKRRQAPPGPRITQRAFGRDWRYPLTNGFREPV"
     gene            10896..12311
                     /gene="argH"
                     /locus_tag="VGC69_02045"
     CDS             10896..12311
                     /gene="argH"
                     /locus_tag="VGC69_02045"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY0294097.1"
                     /translation="MANNAPTPQDQFANKAQAWSARFSEPVSDLVKRYTASVDFDQRL
                     ARHDIRGSLAHAEMLSAQGIIAAQDYADIQRGMQQILAEIDAGSFQWLLDLEDVHLNI
                     EKRLVELVGDAGKRLHTGRSRNDQVATDVRLWLRDEIDGLIDLLRLLRRALATVALEH
                     AATILPGFTHLQVAQPVTFGHHLLAYAEMFGRDADRLADCRKRVNRLPLGAAALAGTS
                     YPIDRDRVARTLAFDGVCRNSLDAVSDRDFAIEFCAAGALVMTHISRLSEELVQWMSP
                     RVGFIDLADRFCTGSSIMPQKKNPDVPELARGKTGRVNGHLVALLTLMKGQPLAYNKD
                     NQEDKEGLFDTVDTLRDTLTIFADMAGGIKVKADNMRAAALQGFATATDLADYLVKRG
                     LPFRDAHEVVAHAVRDCEQRGIDLADLTLAELQAYNAGIEADVHQVLTLEGSVAARKH
                     IGGTAPERVREEAQRVLDETA"
     gene            complement(12319..13284)
                     /locus_tag="VGC69_02050"
     CDS             complement(12319..13284)
                     /locus_tag="VGC69_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294098.1"
                     /translation="MSALHRGLFNRLRYKHLHMLVVLGSSQNLHRAALNLNMSQPAAT
                     RMLHEIEDMFGCPLFERMPRGMRPTALGRQMVRFAEAALNSLDRCAEDLAARQQGGYG
                     YLAIGTIMGAAPDLVMDAVARMKAQNPQMRLRIMGDTSDQVIRLLDQNRIDVAIARRN
                     PETNGDQYVFEPLGNEQLLIVTRSGHPLSRRRKLGLDELVHDWPWILQPPDSPARIAL
                     DQALQRLSLPAPADIIECSSVYSMQQLVQLTDAIMVLSESALRDYLKMGLVKALPVQL
                     TGAFAETLAPFGILMRRNEPLTQEQRTFIALLREQAARQGGRAGR"
     gene            13452..>14082
                     /locus_tag="VGC69_02055"
     CDS             13452..>14082
                     /locus_tag="VGC69_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294099.1"
                     /translation="MKTLPAPLLAKLSWRLLPFLLLMYIMAFLDRANVGFAKQAFQAD
                     TGVGDAAFAFGAGVFFVGYALLEVPSNLIMHRVGARMWMCRIMVTWGLVSAATVFVHD
                     ETSFYVLRFLLGVAEAGFFPGVILYLTYWFPSATRGRAMGFFYFGAPLAFIFGSPLSG
                     LLLELHGVSGWNGWQWLFAVEGLMASIVGIWAYFYLDNRPADAKWLTPDE"
BASE COUNT         2495 a         4561 c         4550 g         2476 t
ORIGIN      
        1 gccatggcga tcatgacgcg ctggcgcatg ccgcccgaga gctggtgcgg gtagcggtcg
       61 aggatggcgc gcgcctcggg gatgcgcacc cgctcgagca tgcgcagggc ctcggcgcgc
      121 gcggcgccgg cgtccagccc ctggtgcagc tgcagcgcct cggcgatctg cgtcccggcc
      181 gtgaacaccg ggttgagcga ggtcatgggc tcctggaaga tcatggcgat gtcggcgccg
      241 cgcacgcgcc gcagcgccga ctcgtcggca cgagccaggt cgagcagctc gccattgcgg
      301 cggcgcaggc gcatgtcgcc gccggcgatc ttgcccccgc cgtattcgat cagccgcatc
      361 agcgacagcg aggtcaccga cttgcccgag cccgattcgc cgacgatggc cagcgtctcg
      421 ccccggtcga cgtggaagga cacatcgctg acggcctcga ccacgcgcga cggcgtcgaa
      481 aatcgcaccg tcaggccgtc tacctggaca accctgcggc cgtccaattc aacatcccta
      541 ttcattgcct tactcctggc gcggatcgag cgcgtcgcgc agcccgtcgc ccagcaggtt
      601 gaagcccagg acgaccagga aaatggccgc ccccggaaag atcgacatcc atggcgcctg
      661 gctcatgaag ttcttggccg tgttcagcat cgagccccac gacgggttgg gcggctgcag
      721 ccccaggccc aggaaggaca ggctggcctc ggcgatgatg gacgtggcga tggtgatggt
      781 ggcctgcacg atgatgggcg gcatgacgtt gggcaggacg tggcgcacga taatgcgcag
      841 atccgaggcc cccagcgcgc gggcgccctg cacgtagtcc tcgttcttga ccgccagcac
      901 ctgcccgcgc gtgagccgcg tgaacttggg cgcggccgac acgccgatgg cgatcatggc
      961 gttgaccagg ctgggcccca ggaaagcggc cagcgcgatg gccaggatca ggaagggaat
     1021 ggccagcagc gcctcggtga cgcgcgagat cacgctgtcg acccagccgc cgaaataccc
     1081 cgccagcagg ccgaagggca cgcccaccac cagggcgatg acgaccgaca ccaggccgac
     1141 catgagcgag gcgcgcgcgc cgaacagcat gcggctgtag atgtcgcggc cgagttcgtc
     1201 ggtgcccagc cagaacaggt gcgacggcgc cttgcgcacg gtcatgaagc tggtcttgag
     1261 cggatcggcg ttggccagca cggaagccag tatggccagc gccacgaaga ccagcacgat
     1321 ggccgcgccc agcatggcgg tgcggttgcg ccggaacttg gcccaggcgc ggctgccgct
     1381 gcgctgcggc acggcgattg cggtcacggt aggagtactc atgaatgcct caaccgggga
     1441 ttgacgacga tgtagagcac gtcggccagc aggttcagca ggatgaagcc cacggccacg
     1501 cacaggacga cgccttgcac cacggcgtag tcgcggttga acacggcgtc gacgatgagc
     1561 ttgccgaagc ccggaatcgt gaacacctgc tcggtgagca cggcgccggc cagcagctcg
     1621 ccgaacagca gggtgaccag ggtgacgatg ggcatgagcg cgttgcgcag cgcatggcgc
     1681 atcaccacct tgtacggcga cacgcccttg gcgcgcgccg tgcgcacgta gtccgcgccc
     1741 agggcctcga gcatggccga ccgggtatgg cgcatcaggt aggcggcgat ggccagggac
     1801 agcacgaagg acggcatcag cagcgtcttg atcgacagcc agaagtcttc cgtaagcggc
     1861 acatagcccg aggccggcag caattgccac tcgaccgaca ccaccaggat gaggatgatg
     1921 cccagccaga aattgggaat cgacatgccg gacagggccg ccatattggc gcccatctcg
     1981 acggccgaac ccttgcgcac ggcggcgatg atgccggccg gaatgcccag cagcagggcg
     2041 aagatcatgg ccatgaccga cagctgcgtg gtgacgggca gcttctgcct gatcagttcg
     2101 ttgacgggca cgtcggtgcg cagggacttg cccaggtcgc cgtgggccac ctgcatgagc
     2161 caggcgccgt actgcaccgg cagcgggtcg ttgaggtggt actttccccg gagatagtcc
     2221 agcacggcgg gatcgcgatc ctcgccggcc atggccagca caggatcgcc gggcaatatt
     2281 ttttgcagca tgaagacgat catcgacacc aggatcaatg tcgggatcga cacgatcacg
     2341 cgtcgcagga tgagtcggag catagggatc agtaaaaacg cggcagcctc caggcttggc
     2401 ccggaggctg cctgccttgc attgccttac ttggcgaagg aaacgccctt gaggcggatc
     2461 ataccgtcgg gagacggctt gaagccgacg accttcttct gcagggcgaa aggccagggc
     2521 tggaagtaca ggaagaagtt ttcctcgtcg ttgtgcagga tggtctcggc ctgctcgtac
     2581 agcgccttgc gctgcgccac gtcgggcacg tggcgcgcct cttcgagcag tctgtcgact
     2641 tccgcgttgc agtagtggcc gtcgttctgc ggccccttgc aggtcacgaa ggcctggatg
     2701 ttgccgtccg ggtcgacgcg gccggaccag ccgcgcagcg acagttcgaa gttgccctgc
     2761 tgggcctggc tcaacaaggc cgcgtactcg gtgggacgca ggctgagatc gaagcccgcc
     2821 tcggccgaca tggcctggat catctgcgcc atggccgtga tggtggtgtc gttgccgaag
     2881 gacagctcgc ccttgaccgg cagggtgacc cccgcttcct tgagcagggc ctttgccttg
     2941 tccagatcgc gggccggcgg ctggaacttg tcgctgaagt acgggctgga cggcgggaag
     3001 ggctgcgccg ccggcgggta gatgccgttg cccaccactt cgttgatggc gttgcggtcg
     3061 atcgacagtt cgagcgcctt gcgtacacga gggtccttga agggcgaggt cttggaaacc
     3121 ggaccgttgc ccacgttgaa gtacacctcc tggtagccca ggccgttgac cggcgcgaag
     3181 accaggctgg cgtcctgctt gacctgcggc acgtccgacg ggcccaggcg ctcgaggatc
     3241 tgcaggccgc ccgagcgcag gttggacagg cgcaccgtgg tgtcggggat gggcaggaac
     3301 accacgcggt cgaagtggta gtccttggcg tcgtagtact tgtcgaactt gtcgagcacg
     3361 atgcggtcgt tctgcacgcg ctcgacgaac ttgtacgggc ccgagcagac cgggctgcgc
     3421 gccacagcgc cgacttcctt gcccgtgaag gaggcgggcg aaagcatcat gccggcgcgg
     3481 tcggacaaag cggccagcag cgtggcgtcg ggctgcttga gcgtcagcac cagcgtggtc
     3541 gggtcgggcg cggccacgct ggccaccgac gtgatctcgc tcttgcgcat gctttccggc
     3601 atggtcattt cgcggtcgat attggccttg gccgcggccg cgtcgaactt ggtgccgtcg
     3661 tggaacacca cgtcgctgcg cagcttgaag gtcagcacct tgtcgtcggg gctccacgac
     3721 caggattcgg ccagctgggg cacgaactcc agcttgtcgt tgatgtcgac gagcttgtcg
     3781 cacagcgagg tgaacacgat gcggctgaca taagtgcgcg cgcggtgcgg gtcgagcgtg
     3841 tccggatctt cctgcaggcc gatgcgcagt tcctgcgcca acgccgtggc cgacgccagg
     3901 gccagcagca cgccccccag caccttgccc atgcgagtgg gtttgaacat cgaaattccc
     3961 cttggatggt ttttcacaac gctattttag aaacccgcgg ccaagccgtc gcggcgcgga
     4021 tcgctcgccg ccacgtagcc ctgttcattg tcgtcgggcg acatgcgcca gatgaactgg
     4081 ccggcgccga agtccatata gggatcgtcc accgacttga gcttgtggcc catctgggcc
     4141 aggccctcga ccacctggcg atccatcgcg ggctcgacgt ccagcgtctg gtcgcggttg
     4201 agcttccagc gcggcgcatc gcaggcggcc tggggctgct ggtcgtagtc gatcatgcgg
     4261 atggccgtct gcaggtggcc ctgcggctgc atgtcgccgc ccatcacgcc gaaactcatc
     4321 accgggcggc cgccctgggt gagaaagccg gggatgatgg tgtggaaggg gcgcttgccg
     4381 ccctcgacca cattggccga aaccgggtcc atcgagaagc ccacgccgcg gttctgcagg
     4441 ctgatgcccg tgcccggcac cacgatgccc gagccgaagc ccatgtagtt ggactggatg
     4501 aacgagatca tcatgccgga ctcgtccgcg gcgctcatgt agatggtgcc accggcggcc
     4561 ggacggccgg acgtgggatg cgccgccttg cccaggtcga tgagcttggc gcgcgaggcc
     4621 aggtaggccg ggtcgagcat ctgctcgggc gtgacttcca tgctgcgcgg gtcggccacg
     4681 tacttgtaga tgtcggtgaa ggccagcttc atggcctcga tctgcacgtg ctgcgactgc
     4741 agcgtgtcca cgcccatgcc cttgaggtcg aagtgctcgc agatgcccag cgcgatcagc
     4801 gcggcgatgc cctgcccgtt gggcgggatc tcgtgcatct cgtagccgcg ataggactgc
     4861 gagatgggct tgacccacat gggccggtag ttgcgcagat cgtccagcgt catggcgccg
     4921 ccgcattcct tgctgaaggc ggcgatgcgt tcggcgatgt cgccctcgta gtagtcgcgg
     4981 cccttgcttt cagcgatgcg gcgcagcgtg taggcggcct cgggcaggcg gaagtgctcg
     5041 ccgaccctgg gcgcgcggcc gttgggcatg aaagcctcgg cgtagccggg ctgcgagtgc
     5101 aattcgtcgg cggccagctt ccacttgtgg gccaccatcg gcggcacggc gtagccgcgc
     5161 tcggcgatct cgatggcggg ctcgaacagc gtctcgaagg gcagcttgcc gaacttctcg
     5221 tgcagggcgg cccagccagc caccacgccg ggcacggtga cggcgtccca accgcgcttg
     5281 ggctggacgg ccaggccatc cgcgccggtg ccgtacttct tcttgaagta gtccacgctc
     5341 caggccgcgg gcgccacgcc cgaggaattc aggccatgca gcgccttgcc gtcccacagg
     5401 atggcgaagc agtcgctgcc caggccgcag gacacgggct cggtcagcgt gatgacggcc
     5461 gcggcggcca cggcggcatc cactgcgctg ccgcccttga gcagcatgcg caggccggcc
     5521 tgcgccgcca gggggtgcga agtcgacacg acattgcgcg cgaacaccgg ggtgcggtgc
     5581 gaagtgtagg ggttggtcca gttgaacgat ttcatggcgc gttcctgcag cgatggggat
     5641 gaacggactg acggcagcat cgctgccgga aaacccttga aattactctc gtgctttaat
     5701 tagatcaatt tgattttttg ttttgttatt aaagattttt ttatgagtcg ccggactttc
     5761 ccacaaagct cgggacaacc cttatgagca cgctgcggtt cctgcgcacc ttcctggccg
     5821 tggtccatca cgggtcgttc tccgaagccg cggagcatgt cgccctgacc caggccgccg
     5881 tgagcttcca gatgcgcgcc ctggaaaacg aactgggccg ggaactcttc gaccgcagcg
     5941 gccgcctggc cctgctcagc gccgccggcc gcgagctcgt gcccgaaacc atgcgcctgc
     6001 tggagctcta cgaccgcatg cggctgccgc ccgccgcgtc cgacaccctg gccggctcgg
     6061 tatcggtcgg cgccatcgtc tcgtgcatgg gcacgctgtc caaggtggtc tcggccatga
     6121 agcgcgagca tccggccctg gacgtgcgca tattttcggg caagtccagc gaactggccg
     6181 gcaaggtcga ggccggcgaa ctggatgcgg ccttcctggt cgaggccggc cggcccacgg
     6241 ccagcacgcg ctggatcacg ctgtacgaag agcccctggt ggtggtggcg ccggcctcgg
     6301 cgccgggcaa gaccgtgcgc gaggtgctgg cccagtcgcc cttcctgcgc tttgaccgca
     6361 cccagcgcac cggccggcag atcgaccgcg tgctgcgccg gctgggcgtc tcgatccacg
     6421 aattcctgga actgaacgcc atcgagacgc tggtcgaact ggtgcgccag gaagtgggtg
     6481 tgaccttgct gccgctgctc aatggcgtca actggcagca gacgccggac ctgcgggtgc
     6541 tgtccctgcc ctcggacctg gggcccttca cccgggccat cggcatgctg gagcggcgcg
     6601 acccggcacg gcaggccatc accacggcca tctgcgcgct cagcggcgca gcctttcgca
     6661 agcggatggc cggcgccgcg acggcctaga cgcaaaacgg ccggcgccag ggccggccgc
     6721 tttccgcgtg acagcgtgtc gtcgtcacgc tcgccggtgc ggatggcctg gattatttcg
     6781 ctacccagcc tgtgttggaa tagccgccgc tttctttgac atagaacgac gtgccggcgc
     6841 cgccatcacg tcttcgatac gtcgaaccta cgcctgcggt aatgacccct tcgggggagc
     6901 ccgcaccagt cgtgtcgaag cattgtgtag atccatctag tgaaagctgg gcccgggatc
     6961 ccaccgtagc cattgttgca acaatagtat tttggttgaa agccctcagc aaagttccgt
     7021 cgccgccgat acgctgccac acatgcgcaa taccgctcgt cgacgtgatc tgttgacccg
     7081 acgagaatat ctggacacca gcgatcaggg tagaggctgg atcaagttga gttgacctgt
     7141 catagcccag ccggaatgca cggctttcat taaaaaccgt gacatcgccg ccagtcgaca
     7201 catgcaccgt attcgtcgtg gcatccacgg aaatgtgacc atccgaataa gtaaccttac
     7261 atgccacgca cgttgcatcg aatccgacaa gataagacgc atcgctaagg cttgggtcat
     7321 ccacataatg tgcaggattg atgacgtgca cattggaaca tcccgaaatg gcgatgggcc
     7381 accgatccgg gcttggcgtc gagcctgtat ggacaaattg gaaatccctg atcttgatgt
     7441 cagactgccc tacgcaaagc aatggctgct ttccagtatc ggtatagtcc ccgaccacaa
     7501 ttacattatt caccgtgaca ttcttcgcgt tcgcagcatt cgtatcggta cgcatcaaga
     7561 tgccatacgc catttgtccg cgaatatcga tttccacgcc atcgaccacg aagttggatg
     7621 tgtccgtgat ttccagcatg ttcgtgccga caccggtagg attgttcaac gcgtcttgta
     7681 agcaaacagc cgcatagttc gaaagtgaac cgctttgcac atccacgata tcaataccac
     7741 ggccacattg attcaatgcc acgttagaaa ttacgaccac ctcgcctttc cgcactttca
     7801 gggttgaatt gtgatctttg tacccagaaa tgtgagaaat attgatagtg tgaggcgcgg
     7861 cccctatcct gttcgtcaca tacaagtaat gccctggatc ggttgaacca tcggcattgg
     7921 tgcttatttt tgtattcttc gaaccaccgt gaataatcga tatccgttca ggctgttcaa
     7981 ccaaaaggcc gaaacagcaa gtatccgtgt agtagtcccg cgtacgcaga tcaccgtaca
     8041 gggtttcctc atcggtgtaa tcaccgcagt aatgaacccc aacgttgatc tgagagcagg
     8101 ttacatcatc aataacaagg ccaccattac catcatactg tcggatgaat gaaaatgcgt
     8161 tgttgcccaa tccggccagt ccgctgcttg gcgtgatcag gggctgcttg gggacgtctt
     8221 gcaacaggcc atactcatct ccataaaccc aaattccacc aattcgcaaa tttgctcccc
     8281 gttgcgcgat gaattcgagt ttgtttgatg tgggcaccca aatggttgca ccactgcctt
     8341 ggccgacaat gctgatatta ggatccaatg aacttacggt agcggccgtt ccttcatcag
     8401 gagcgataat ggaagcataa ggcgcatgca caaacgtgcc gcttccgtat tcgatgacgc
     8461 caccgccgaa tggacgcaga tggagaatcg cacgcatcag cgccgctgag tagtccgtct
     8521 ctccggtaac aaaaaaatca tcaatataaa cgtgcgaatc catatatccc tcttctttag
     8581 tcaggaaaaa agacctcgcg ctattacacg atcttctttg gttttattta caaaataaag
     8641 tctaaagagc cagacgaaga ctcagtccgc agggcatcct gctttggtca tacgaaatcc
     8701 ccaaaagcaa aacggccggc acgccatgtg ccggccgtct gccagaatcc gaaacagatt
     8761 acagcgcgtc gtcgtcgctc tcgccggtgc ggatgcggat ggcctgctcg accggggtga
     8821 cgaagatctt gccgtcgccg atcttgccgg tgcgcgcggc cttgagcacg gcctcgatgg
     8881 ccgtctcgac gcggtcgtcg ggcaggacca cctcgacgcg gatcttgggc aggaaatcca
     8941 cgacatattc ggcgccgcga tagagctcgg tatgcccctt ctgtcggccg aaacccttga
     9001 cttcaacaac cgtgaggccg ctgacgccgg cttcggccag cgcctcgcgc acctcgtcga
     9061 gcttgaaggg cttgataatg gcggtgactt gtttcatgtg cgtcccttgt tgtatggctg
     9121 caggatgcct gccgcgcgac ggcctagacc ggctcgcgga aaccgttggt gagaggatag
     9181 cgccaatcgc ggccgaatgc gcgctgcgtg atgcgcggtc ccggcggcgc ctggcggcgc
     9241 ttgtattcgt tgatgcggat caggcgcagc acctgctcga ccgccgcggc cgggtagccg
     9301 gccgccacga tctgcgccgg cgactcattg tgctccatgt agcgggccat gatgccgtcg
     9361 agcacctcgt aaggcggcag gctgtcctgg tcggtctggt cggggcgcag ctcggccgaa
     9421 ggcggccggg tgatgatgcg ctcggggatg acctcgccgc gcgtgttgcg ccagacggcc
     9481 aggcgataga ccagcgtctt gggcacgtcc ttgatgacgg cgaagccgcc ggccatgtcg
     9541 ccgtacagcg tacagtagcc ggtggtcatt tcagatttgt tgcccgtggt gagcaccagc
     9601 cggcccgtct tgttggacag ggccatgagc agcgtgccgc gcacgcgggc ctggatgttt
     9661 tcttcggtgg cgtccaccgg caggccggcg aactggccgg ccagcatgga ttcgaaggcc
     9721 tggaaggccg gcgcgatggc gatctcgtca tagcgcacgc ccagccggcg ggccatgtcg
     9781 ctggcgtcga gctgggagat gtcggcggta tagcgcgagg gcatcatcac cgcgcgcaca
     9841 ttgtcggcgc ccagcgcgtc gaccgcgacg gccagcacca cggccgagtc gatgcccccc
     9901 gacaggccga tgatggcgcc gggaaagccg ttcttgccca ggtagtcgcg cacgcccagg
     9961 accagggcgt tccagacttc ttcttcctgg ctggtttcga aggcctcggg caggccgtcc
    10021 agggcccgca ccgaacccgc cgcgtcgatc tcgaccagcc ccaggtcctc ggtaaagccg
    10081 ggcagctgcg ccatctgcct gccctcggcg tccagcacga aggacaaccc gtcgaagacc
    10141 agctcgtcct gcccgcccac caggttggcg tagaccaggg cgcagcccgt gccctgcagg
    10201 ctgcggcggg ccacctcgac gcgctcttcc tgcttgccct ggttgtaggg cgaggcattg
    10261 ggcaccagca gcacgcgggc gccggcggcc gccgccgcgc gcggggcgct gtcgaaccag
    10321 atgtcttcgc agatgttcac gccgaaccgc acgcccttga cctcgaaaac cagggaagac
    10381 tcgcccggct cgaagtagcg cttttcgtcg aacaccgaat aattgggcag ctcccgcttg
    10441 gcgtaggtgc ccagcacgcg gccttcgcac aggacgctgg cggcattgtg cgggcggcct
    10501 tcgcggcgcg ccgcatggcc caccaccaca tgcaggccgg ccaggccggc caggtcggcg
    10561 cacagctgct gcagggcgcg cgcctgcgca tcgatgaagg acgggcgcag cagcagatct
    10621 tcgggcggat agccggtgag cgacagctcg ggcgtcacca gcacatcggc gccctgggca
    10681 tgggcctcgc gggccgcctg caggatcttg gcggcattgc tggccaagcc tccgacgacg
    10741 gtattgatct gcgcgaaagc gacacgggcg gcgctcatgg gggacaggtt ccgtagacac
    10801 agaatgggga cggggaaaat tatcgcatgc ctctcccccg ctcccgccgt ccggcccggg
    10861 acgggcggca accagcctct ataatcggcg ccatcatggc aaacaacgca cccactcccc
    10921 aagaccagtt cgccaacaag gcgcaagcct ggtccgcccg gttctccgag ccggtttccg
    10981 acctggtcaa acgctatacc gcctcggtgg atttcgacca gcgcctggcc cgccacgaca
    11041 tccgcggctc gctggcccat gccgaaatgc tgtccgccca gggcatcatc gccgcccagg
    11101 actacgccga catccagcgc ggcatgcagc agatcctcgc cgagatcgac gccggctcct
    11161 tccagtggct gctcgacctc gaagacgtgc acctcaacat cgagaaacgc ctggtggagc
    11221 tggtgggcga tgccggcaag cgcctgcaca ccggccgctc gcgcaacgac caggtggcca
    11281 ccgacgtgcg cctgtggctg cgcgacgaga tcgacggcct gatcgacctg ctgcgcctgc
    11341 tgcgccgggc gctggccacg gtggcgctgg aacacgccgc caccatcctg cccggcttca
    11401 cccacctgca ggtggcccag ccggtcacct tcggccacca cctgctggcc tatgccgaga
    11461 tgttcggccg cgacgccgac cgcctggccg actgccgcaa gcgcgtcaac cggctgccgc
    11521 tgggcgccgc cgccctggcc ggcaccagct accccatcga ccgcgaccgc gtggcgcgca
    11581 ccctggcctt cgacggcgtg tgccgcaact cgctggacgc cgtgtccgac cgcgacttcg
    11641 ccatcgagtt ctgcgcggcc ggcgccctgg tcatgacgca catctcgcgc ctgtcggaag
    11701 agctggtgca atggatgagc ccgcgcgtgg gcttcatcga cctggccgac cgcttctgca
    11761 ccggcagcag catcatgccg cagaaaaaga accccgacgt gcccgagctg gcccgcggca
    11821 agaccggccg cgtcaatggc cacctggtgg ccctgctgac cctgatgaag ggccagcccc
    11881 tggcctacaa caaggacaac caggaagaca aggaaggcct cttcgacacg gtcgacacgc
    11941 tgcgcgacac gctgacgatc ttcgccgaca tggcgggcgg catcaaggta aaggccgaca
    12001 atatgcgcgc ggccgccctg cagggcttcg ccaccgccac cgatctcgcc gactacctgg
    12061 tcaagcgcgg cctgcctttc cgcgatgcgc acgaggtggt ggcgcacgcg gtgcgcgact
    12121 gcgaacagcg cggcatcgac ctggccgacc tcacgctggc cgaactgcag gcctacaacg
    12181 ccggcatcga ggccgacgtg caccaggtgc tgacgctgga aggttcggtg gcggctcgca
    12241 agcacatcgg cgggacggcg ccggagcgcg tgcgcgaaga agcgcagcgg gtgctcgacg
    12301 agacggcttg agccccgcct agcgtccggc cctcccaccc tgccgggccg cctgctcgcg
    12361 cagcagcgcg atgaatgtcc gctgctcctg cgtgagcggc tcgttgcggc gcatcaggat
    12421 gccgaacggc gccagcgttt cggcaaacgc cccggtgagc tgcaccggca gcgccttcac
    12481 cagccccatt ttcagatagt cgcgcagcgc cgattccgac agcaccatga tcgcgtcggt
    12541 gagctgcacc agctgctgca ttgaatagac cgagctgcat tcgatgatgt cggccggcgc
    12601 cggcagcgac aggcgctgca gggcctggtc gagcgcaatg cgcgcgggac tgtcgggcgg
    12661 ctgcaggatc cacggccaat catgcaccag ctcgtccagg ccgagcttgc ggcggcgcga
    12721 caaggggtgg ccgctgcgcg tcacgatcag cagttgctca ttgcccagcg gctcgaacac
    12781 atattgatcc ccgttggtct cggggttgcg ccgcgcgatg gcgacgtcga tgcgattctg
    12841 gtccagcagc cgtatcacct ggtcgctggt gtcgcccatg atgcgaaggc gcatctgcgg
    12901 attctgcgcc ttcatgcgcg ccaccgcatc catgaccagg tcgggcgcgg cgcccatgat
    12961 ggtgccgatc gccaggtagc cgtagccccc ctgctggcga gcggccaggt cctcggcgca
    13021 gcggtccagg ctattgagcg ccgcctccgc gaagcgcacc atctggcgcc ccagcgccgt
    13081 gggccgcatg ccgcgcggca tgcgctcgaa cagggggcag ccgaacatat cctcgatctc
    13141 gtgcagcatg cgcgtggcgg ctggctgcga catgttcagg ttcaacgcgg cgcgatgcag
    13201 gttctggctg gagccgagca ccaccagcat gtgcagatgc ttgtagcgca ggcggttgaa
    13261 caagccgcgg tgcaaagcgg acaaggcgtt gttgcgtggc atggcgggtg gagagcgttg
    13321 gatttgaacg atacccaaaa ggtatcgctt gtgaacgaaa ttcgatttgt ggtgcatagc
    13381 acggacacgt acagtttccg ccgacaacaa taaaaaatct atacacaaca acatgaatat
    13441 tggagacaac catgaagacg cttcctgccc ctttgctcgc caagctttcc tggcgcctgc
    13501 tgcccttcct gcttctgatg tacatcatgg ccttcctgga ccgagccaac gtgggcttcg
    13561 ccaagcaggc attccaggcc gataccggcg tcggcgacgc ggccttcgcc ttcggggccg
    13621 gggtgttctt cgtgggctac gccctgctcg aagtgcccag caacctcatc atgcatcggg
    13681 taggcgcgcg catgtggatg tgccgcatca tggtgacgtg ggggctggta tcggccgcga
    13741 cggtgttcgt gcacgatgaa accagtttct acgtcctgcg ctttttgctg ggcgtggccg
    13801 aggccggatt cttccccggc gtcatcctct acctgaccta ttggttcccg tcggccacgc
    13861 gcggccgggc catgggcttt ttctacttcg gcgcgccgct ggccttcatc ttcggcagtc
    13921 cgctgtcggg cctgctgctg gagctgcatg gcgtgagcgg ctggaacggc tggcaatggc
    13981 tgttcgcggt cgaggggctc atggccagca tcgtgggtat ctgggcgtac ttctacctcg
    14041 acaaccggcc cgccgacgcc aagtggctga cgccggacga gc
//
LOCUS       DASWPO010000039        25321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_7655, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000039 DASWPO010000000
VERSION     DASWPO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 25321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25321
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7655"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VGC69_02060"
     CDS             <1..342
                     /locus_tag="VGC69_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEY0294100.1"
                     /translation="WGLLFGGVFLAVPVLGSVVVLGHLAAIVVSIVEGAVVMGGLSAI
                     GAAIASLGIPKDSVIKYETAVKANGFLVMGHGTSEEISKAKAVLGALNPSLLDVHEGL
                     AQASSAGHAIS"
     gene            complement(452..1321)
                     /locus_tag="VGC69_02065"
     CDS             complement(452..1321)
                     /locus_tag="VGC69_02065"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HEY0294101.1"
                     /translation="MKTRSEDFCVQEGDDVNLGKWPTTVDPLYSSKEHYQELIAAHVA
                     RLSSLQQLFYASNRHALLLIFQAMDAAGKDGAIKHVMSGVNPQGCQVFSFKHPTPIEL
                     EHDFLWRTTRDLPARGQIGIFNRSYYEEVLIVRVHPEILLGEGLPDALLDKESVWHGR
                     YRSIVDLERHLDRNGTHIIKFFLHLSKAEQRRRFLARIDDPDKNWKFSAADIAERKFW
                     KQYMKAYEQCLGATSTHGAPWFVVPADDKKNARLIISRIILDALESLKMTYPQSTASR
                     RRELQAIRKGLAG"
     gene            complement(1399..3732)
                     /locus_tag="VGC69_02070"
     CDS             complement(1399..3732)
                     /locus_tag="VGC69_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY0294102.1"
                     /translation="MTTSPPEVPSESGLSSAEARRKLAESGPNATPDVSAHPLRMALG
                     KFWAPVPWMLEAAVVLECALGKFVEAGIIAGLLVLNAALGFFQESRAQATLTALKSRL
                     ALNASVRRDGAWSTVPAADLVPGDVVKLSLGAVVAADAKLVSGNALLDHSMLTGESVP
                     IEGAAGVQTYAGALVRRGEAVAVVTATGVHTRFGRTAELVRTAHAASSQQKAVLLVVR
                     NLAIFSGVVIVMLLACALYLHLSWADIVPLVLTAVLASIPVALPATFTLSAALGARAL
                     AGRGVLSTRLAAVDEAGTMDILCTDKTGTLTRNALTVTTVQPMPGFDSQHVLTFAALA
                     SAEGGGDPVDAAIRAAAADHLASDAPKLTRFTAFDPDKKMSEASATDSTGNALRIVKG
                     AFAVITGRAEPLPTAAAQANELEGKGFRVLAVAAGAPAALKLVGMVALSDPPRDDSAA
                     LITELHGLGVRAVMVTGDAPATAGIVAHAVGLDGATCPPGPIPDSVKPESFTVFAGVL
                     PEDKYKLVKAFQKSGHVVGMCGDGANDAPALRQAQMGIAVSTATDVAKSAAVMVLTEP
                     GLTGIVAAVKEGRVTFQRIQTYTLNSIIKKITTALLLVFGLIMTGHAILTPLLMVILM
                     IAGDFLAMSLTTDNVDSSASPNTWRIGNLTIAGVVIGVLLLAFCTGVLAIGRFEMDLG
                     PGALQTLTFVVLVFGSQATLYAIRQRRRMWGTRPSLWLIASSVADVVIAVILAVGGIA
                     MTSLSVAVVAGVLAATVVLALVLDALKIPIFARLNIT"
     gene            complement(3899..4534)
                     /locus_tag="VGC69_02075"
     CDS             complement(3899..4534)
                     /locus_tag="VGC69_02075"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY0294103.1"
                     /translation="MSQESNHGGKLIERYTANERTNHWITAICFVLLALSGLALFHPA
                     FFWLTNLFGGGPWTRILHPFVGVVMFVSFFIFAIRMFKYNCMTAADRQWLGQIKDVLT
                     NREENLPEVGKYNAGQKMLFYVLILCMAGLLLSGIVIWRAYFSMYFPIWVIRAAAVLH
                     ALCGFAIICSIIVHIYAAIWVKGSVGAMVRGKVTPGWAWRHHRTWFREVRK"
     gene            complement(4537..5430)
                     /gene="fdxH"
                     /locus_tag="VGC69_02080"
     CDS             complement(4537..5430)
                     /gene="fdxH"
                     /locus_tag="VGC69_02080"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEY0294104.1"
                     /translation="MSLQSLDIKRRSSTTTPPPGVREPSTGTVAKLIDVSKCIGCKAC
                     QSACMEWNDLRDEIGTNVGVYDNPADLSEHSWTVMRFSEYENEETGNLEWLIRKDGCM
                     HCEDPGCLKACPSPGAIVQYTNGIVDFHEENCIGCGYCVTGCPFNVPRISKKDSKAYK
                     CTLCSDRVAVGQEPACVKTCPTGAITFGTKEDMHQHAAERIEDLKSRGFEQAGLYDPQ
                     GVGGTHVMYVLHHADKPMLYHGLPENPRISPMVSFWKGVAKPLGVAIMAFSALAGFFH
                     YIRIGPNETSEEDERKAEQEA"
     gene            complement(5441..8503)
                     /gene="fdnG"
                     /locus_tag="VGC69_02085"
     CDS             complement(5441..8503)
                     /gene="fdnG"
                     /locus_tag="VGC69_02085"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7913..7915),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEY0294105.1"
                     /translation="MVNMNRRQFFKVTGATLAGSSLALLGAAPGTALAEVRQYKLSRM
                     TETRNTCPYCSVACGILMYGLGDRAKNAEASIIHIEGDPDHPVNRGTLCPKGASLIDF
                     VHSPNRLKYPEYRAPGSDTWRRMSWDEALDRIAKLLKADRDANFVEKSDDGKTVNRWL
                     TTGVLAASASSNEVGYLTHKVVRSLGVLAFDNQARVUHGPTVAGLAPTFGRGAMTNHW
                     VDIKNADVVLIMGGNAAEAHPCGFKWVTEAKAHNKAKLIVVDPRFTRSASVADFYAPI
                     RTGSDIVFLGGAIRYLLEKDQIHHEYVKNYTDFSFIVREDYAFDEGVFSGYDAEKRTY
                     AKGSWDYERGDDGFVKTDPTLQHPRTVYQLLKQHYDRYTPEMVERVCGTPRDKFLKVC
                     EMLGSTATPGRAATILYALGWTQHSIGSQIIRTGAMLQLLLGNIGIAGGGMNALRGHS
                     NIQGLTDLGLMSNLLPGYMTLPNEPEQDFGQYIDARASKPLRPGQLSYWQNYKKFHVS
                     FMKAWWGSAATAENNWAYDYLPKLDKMYDMLQTYELMNQGKVNGYLCQGFNPLAAAPD
                     KAKLLSAFAKLKFMVVMDPLNTETSEFWKNYGEHNDVDSSKIQTEVFRLPTTCFAEEE
                     GALVNSGRWLQWHWKGAEAPGEARSDLEIMSGLFLRLRDMYRKDGGKFPDPIVNLSWP
                     YSNPESPTAVELAKEYSGRALVDLTDASGAVVRKAGEQLSGFAELRDDGTTLSGCWIF
                     CGAWTQAGNQMANRDNSDPTGVGQTLNWAWAWPANRRVLYNRASCDLEGKPYNPKRAL
                     IAWNGKGWGGADIPDFVATSKPADGMGPFIMNPEGVARFFARAGMAEGPFPTHYEPFE
                     TPLGYNPVYPQSKTATNNPAARVFKGDLEAMGKVEQFPYVGTTYRLTEHFHYWTKNVE
                     LNSILQPQQFVEIGESLAQELGIANGSQVKVSSNRGYIKAVALVTKRIKALDVDGKKI
                     HHVGIPIHWGFVGIAKPGFLANTLTPFVGDGNSQTPEFKSFLVKVEKA"
     gene            8729..9235
                     /locus_tag="VGC69_02090"
     CDS             8729..9235
                     /locus_tag="VGC69_02090"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0294106.1"
                     /translation="MDTPSHQLSMTILMTPDTANFSGNVHGGTILKLLDQAAFACASR
                     YAGTYVVTLSVDQVVFRQPIHVGELVTFLASVNYTGRTSMEIGIKVIAEDIRKKIARH
                     ANSCYFTMVAVDDQGQPISVPPLEPGDLEEAQRFEGAKHRRALREEMEKRHQDIKQTI
                     QDCNGKPG"
     gene            9387..10988
                     /locus_tag="VGC69_02095"
     CDS             9387..10988
                     /locus_tag="VGC69_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0294107.1"
                     /translation="MQAPASLPDRVPVLIAGGGPVGLSLAALLAGQGIASLTIEADAE
                     YCSGSRAICMSRRSQEILGWVGADVPLVEKGLSWTGGRSYFRHEEVLHFEMPSDPLQR
                     YAPMVNIQQYYIEEYSHRAMQRHGGLAQVAWASRVTGLKDLGDAVEVAVEAGGQRRTV
                     RADWVVACDGGRSTVRELLDLHLRGMQYEGRYVIVDIEQDTRRPVERLAWFDPPSNPG
                     STILMHRQPDNVWRIDYQVDDDADAAEAIKPENVLPRVQSHLDMIGETAPWKPLWISI
                     YNAKCLTLDSYRHGRVLFAGDAGHLVPIFGVRGLNSGLDDAGNLAWKLAWVLRGAAPD
                     SLLDTYSQERVYATRQNIEYGAKSTEFMAPPDYGFRLLRDATLRLSLVEPSVSELINP
                     RQSSPITYAGSPLNQPDGAPACGAAAAPGQPAPEALLARDGRPVHLTSLFGRGFVALV
                     NGEAPELSLLQSLQAKTRGTPAPFEVVEIGAGAYADPHGQIREGYGAQPGLVYLIRPD
                     GYVMGRWADAGAAASAMRALAPFHA"
     gene            11007..11186
                     /locus_tag="VGC69_02100"
     CDS             11007..11186
                     /locus_tag="VGC69_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294108.1"
                     /translation="MNSTDLDTAYAALAHALTRVGEARAPLFLAMLCLSLMSRNPDVG
                     QVLALMEQAEAKQSP"
     gene            11287..12261
                     /locus_tag="VGC69_02105"
     CDS             11287..12261
                     /locus_tag="VGC69_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294109.1"
                     /translation="MRILAHAAAALLLAAGLTPAHAADDYPAAYPIHWVVPYAAGGGS
                     DIVARNLQEGMSKVLSQTIIIDNKPGAATAIGAEYVAHAKPDGYTILSGDTATLAANP
                     PLYPRLAYDPQRDFAPVGLMARFNMILVVNPSVPAHGMKEFMAWARAQKDGVSYGTPG
                     AGSPHHLATELFRARTGLNLKHVPYRGAAPAVQDVMSGQVPMMFVDSASGQQYIASGK
                     LRALAVASKDRLPGMPDVPTLIESGLPDFEAYAWQGLVVPKGTPQPVIDKLNAALNET
                     LKSPTVIEKFKAMGLEVIPSTPRALDDYARAERAKWTQVIQDAHITIE"
     gene            12274..12753
                     /locus_tag="VGC69_02110"
     CDS             12274..12753
                     /locus_tag="VGC69_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0294110.1"
                     /translation="MPAERPSDTEPLPDLERFLTYRLHVVNKLSDRDTNRAYLDKLEL
                     PLGEARCLAAIGRFAPLSVKDLARAANLDKGQASRSAKSLAERGLVDKAASATDARGV
                     LLTLTPAGADMYERVIRLIEARNREIFGCLSPAEREHLGRALDRIARHLADGQDQDG"
     gene            12805..13665
                     /gene="ada"
                     /locus_tag="VGC69_02115"
     CDS             12805..13665
                     /gene="ada"
                     /locus_tag="VGC69_02115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEY0294111.1"
                     /translation="MDTPLADIMPNATQHAALVAAACRLIEAADAPPSLAELAARVGL
                     SPHHFHRLFKAETGLTPKAYAAAHQARRLRAGLAGQGTITDAIFEAGYNANSRFYEQA
                     DQRLGMRPRDYRAGGANTDIHFAVGQCSLGAILVAGSPRGICAILLDDDPDALVRALQ
                     DQFPRARLIGADRDFEQWVAQVVGLVETPSVGLNLPLDVRGTAFQERVWRALRDIPPG
                     TTATYADVARRIGMPSAVRAVAGACAANKLAVAIPCHRVVRSDGSLSGYHWGVARKRA
                     LLDREAEQAA"
     gene            13785..14792
                     /locus_tag="VGC69_02120"
     CDS             13785..14792
                     /locus_tag="VGC69_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY0294112.1"
                     /translation="MKAIAYTQHGLPADDPKALIDLDLPTPEPGPRDLLVAVRAISVN
                     PVDTKIRMGVATEAPRILGWDAVGTVKAVGSAVSLFKPGDEVYYAGTLLRPGTNAEFS
                     LVDERLAGAKPKSLSDAQAAALPLTTLTAWELLFDRLGVPEGGGAGQSLLIVGAGGGV
                     GSILVQLARKLTGLTVIGTASRAETRQWVQDLGAHHVIDHGKPLAGELARVGVPQVDL
                     VAGLTHTEEHYAQIVESLAPQGKLALIDDPAVLDAVPLKRKSISLHWELMFTRSLYET
                     PDMVRQHEILERAAQLIDSGVLKTTLGDHYGKINAANLRRAHAMLQSHKARGKIVLEG
                     F"
     gene            14795..14986
                     /locus_tag="VGC69_02125"
     CDS             14795..14986
                     /locus_tag="VGC69_02125"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HEY0294113.1"
                     /translation="MPYVRVEMVEGRSDEQKAKLAQAITAAMVEHAGAPADSVFVVLE
                     DVKKNNWAIGGTLISQRKK"
     gene            complement(15041..15526)
                     /locus_tag="VGC69_02130"
     CDS             complement(15041..15526)
                     /locus_tag="VGC69_02130"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY0294114.1"
                     /translation="MTQAPADAELTRLVYQEARLIDQGRFEEWYALFDEDGLYWMPLT
                     PGQQSGESEPALMYEDKMLLRLRLDRMKDPKAHSLHPAVRCLHVLQAPEVTSRDEAGG
                     RYALSTPFLYVETQGDAQQVLAATAEHLVASTPQGLRIKIKKVLLLNCDAALPAIQLL
                     P"
     gene            complement(15551..16888)
                     /locus_tag="VGC69_02135"
     CDS             complement(15551..16888)
                     /locus_tag="VGC69_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0294115.1"
                     /translation="MSLSLSMLPPDAGDESVADLVEPDRIHRDVYISERVYRLEQERL
                     WKRAWVFLGHDSLVPNAGDYYTAEIAGQSLVMLRQADGKVAVLFNRCAHKGSRLLHER
                     QGNAGKFLRCPYHAWTYKLDGKLMSIPLKNDYEGTGLSDSPSGQGMAAPGGVRNYKGF
                     VFTRISAAGPDFDSYFSPAVLATLDNMVARSPVGELEVVGAPLRNVMRCNWKMYLENI
                     NDTVHPISTHESAATAAGKVGKSLPEGDARTSLAMEQLLPFGAGYSFYSDMGAKVLPN
                     GHSVLGTKASIHTGYAPLPEYEAELAGLYGEEEARELLSFSPQNTVFYPSAAFKTSPS
                     LMRVLRPLGPDRTLIEAWAMVPKGAPQRLRQRVASYVRLVFSPMSVVAHDDVHLFESQ
                     QIGLGSDGNEWVSLHRGHQAGETQTPSLALENGNNEIMMRNQYRGWLSLMDPA"
     gene            complement(16909..18774)
                     /locus_tag="VGC69_02140"
     CDS             complement(16909..18774)
                     /locus_tag="VGC69_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294116.1"
                     /translation="MAAVEFYELTLSLNVAGKRADVLRDIALHVEPGAMLGLVGESGA
                     GKSMIGRMIAGEIPPGFAVTHGFVRFEGQDLMALPRAQHRALLGRRIAFIPQEPLSGL
                     NPVLTVRQQIFEHLAHVGAPKAQWRRMALDRLAEVGLPNGEAILARYPHQLSGGQCQR
                     ILIAMAFCGEPALIVADEPTTALDVMTQAQIMRVLREQQQRHGTAVILITHDLRLAAH
                     VCDQVAVLYAGEVVESGSAAQVLDHPVHPYTLSLKSATPALQGGRVALPSLPDFMPAV
                     GDFPDIAGCRYYSRCAVRQPGCERRPPALRPRGAGRLARCLELIPAIDRQAADPAPSL
                     PAGDGQARPLVEFRNVSLSYPGRASGWKKAEPIHVVQPVSFTVAPGEFLGIVGESGSG
                     KTSMARLLMGLVPPTAGSILIDGQDRAAANAEQARDIRKCVQMVFQNPDSALNPRRRV
                     ADLVTQILEADGAGTEQAADRQAAARRLMQAVGLSPSSLGRLPSQLSGGQKQRVNIAR
                     ALCLTPRMLVADEIVSGLDVSVQALILNMLLRLNRELGIGLVFISHDLAVVRYLCNRV
                     MVMHRGVVVEAGETEQVFTSPRHDYTKALIAAVPPETGDAAWPPVLPPALSPVPA"
     gene            complement(18778..19668)
                     /locus_tag="VGC69_02145"
     CDS             complement(18778..19668)
                     /locus_tag="VGC69_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294117.1"
                     /translation="MSSVAMQSGSRWRPLLRSLGRSPKALIGGAIVLLLVLVAVFAPW
                     IAPNDPLSQDLLSPLLPPAWMADGTAQYPLGTDSLGRCVLSRLIFGSRVALWVGLCSA
                     VGTMLLGCALALLGSYFGGKVDRGISYLVDLWMSFPPVVLSLILMVGLGPGVGNVILS
                     VVLVDWTRFCRVVRAEALVIRRRDYVVASRLMGFSHLRIMLREMLPGLVPLIITLFSL
                     EMGVAIVVEAILSFVGLSVPASTVAWGVMIADARGYMQENIWNLIPPVCGIVVTVLGF
                     NLLSDGLRQALDPRANTSRV"
     gene            complement(19668..20684)
                     /locus_tag="VGC69_02150"
     CDS             complement(19668..20684)
                     /locus_tag="VGC69_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294118.1"
                     /translation="MLAVLARQALARVAVAIPMIFALSVVVFVVLRLLPADPLAMMLP
                     PTATQAEIDTLRAALGLDRSLPVQYGIWLVHALHGDLGRSITSGVEVTRMIATSLPAT
                     LELSIVSLVFSLVISIPGGLLLYALHRRRGELLADGFVVLLISVPSFLWGIFLMLGLG
                     VMWPLLPFSGRFGADVAPPGATGFVLIDFLLRGDFRAWGNAFSHLVLPALALALGFAP
                     LIIRVLRASLLEAAGEQYTMVARLRGQSDTRIVLRHMLKNAALPTLTLIGVQFGFLFG
                     GTLLIEMIFSFPGLGNLMVQAVRNHDLPLIQGIALVFCVIILIINTCIDCLYTVVNPR
                     LRDA"
     gene            complement(20688..22229)
                     /locus_tag="VGC69_02155"
     CDS             complement(20688..22229)
                     /locus_tag="VGC69_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294119.1"
                     /translation="MSTRLLTAPARRALASCLVALGCAAAAPTASAAGDELTIAYPVD
                     LPTWDPTAGTFPAGQSLYKAVFDSPLMINDKLEVTPRLIKSWKWLDDKNTSLDIVLRD
                     DVTFQDGSKLTTEDFKYSFLDRPLADKKLVIGSTFTSKFASIDIKSPTEAVLNFKNPM
                     PTAPNWLGFLTAYILPKAYIEKVGVDQFLEHPIGAGPYKLVSYERGSRAVLEAYDGYW
                     GPKPAIKRVVFDFVPDTSARVAMLESGRADVAVQIPVREVNRLAKTDAIAAKVYPYTQ
                     VFMLQMPSYVPELQNEHVRQAMQLAIDKQALSRAFYANSAKPISVLALPGTPAYVDGF
                     DVKFDKKAAAAELAQAGYGPGKPLEIHFLSANGSVPGDYEMARAIVQMWSQIGIKADL
                     EEVTLAKYLDLSHSGKLPGPMLYSWDNATGDPEIFSGMILNPSLPFSAWKQPELADTF
                     KQLAAMGQDQRIAGYRDVEKKATENAWSIPLLQSVSTIAYKKSLNLSTYQTGYIMPQE
                     YSWKK"
     gene            22497..23186
                     /locus_tag="VGC69_02160"
     CDS             22497..23186
                     /locus_tag="VGC69_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0294120.1"
                     /translation="MQAPGSPGKKPNLRERVYQMLRERIQMGLIGHNDRLVDHDIAHD
                     MHISRMPVREALMQLKSEGVLEGTARGFILRRHSLQQINEIFEIRILLEPHAAGLATA
                     NASPAFLARMKAALDAAEQASRRGDWESFMRINADYRTAWIAMVPNREMADTISRFID
                     HVQVVRLMTMQDDTVRNIILDGMRGLYEAFLSGDANLVRERMTTHCRTASASYYQCYQ
                     RLYDTGPPPTN"
     gene            23325..>25321
                     /locus_tag="VGC69_02165"
     CDS             23325..>25321
                     /locus_tag="VGC69_02165"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0294121.1"
                     /translation="MSTTSKIIYTLTDEAPALATYSLLPIVQAYAGPAGIAVETRDIS
                     LSGRIIATFPEVLTDAQKISDELAYLGQLAAKPEANIIKLPNISASIPQLKAAIKELQ
                     GKGYKLPEYPDEPKNDAEQDVKTRYDKIKGSAVNPVLREGNSDRRAPLSVKNYARKHP
                     HKMGAWAADSKAHVADMPSGDFYGSEKSALIAAEGAVKITLHAKDGATRVLKETTKVK
                     AGELIDSSVMSKKALRAFITEQIADARAQGVLFSVHLKATMMKVSDPVIFGHFVSVYY
                     EDVLAKHADTLASIGFDPNNGIGDLYTRLATLPSAKQAEIKADIAAEYAKRPALAMVN
                     SDRGITNLHVPSDVIVDASMPAMIREGGKMWNAEGKAQDAKAIIPDRCYSGVYQAVIE
                     DCKVNGAFDPVTMGTVPNVGLMAQAAEEYGSHDKTFVIPADGTVKVTDASGAVLLEHA
                     VEAGDIWRMCQTKDAPVQDWVKLAVTRARLSNTPAVFWLDPARAHDAQVIAKVERYLK
                     DHDTTGLDIRILSPIEATKFSIERIRKGLDTISVTGNVLRDYLTDLFPIMELGTSAKM
                     LSIVPLMAGGGLFETGAGGSAPKHVQQFLEEDFLRWDSLGEFMALAASLEHLGQYAGN
                     AKATILAKTLDEATGKFLDTDKSPARKVGGLDNRGSHFYLAL"
BASE COUNT         4400 a         8612 c         8268 g         4041 t
ORIGIN      
        1 tgggggctgc tcttcggcgg cgtcttcctc gcggttccgg tcctggggtc ggtcgtggtc
       61 ctggggcatc tggcggcgat cgtagtgtcg atcgtcgagg gcgccgtcgt catgggcggg
      121 ctgagcgcga ttggcgccgc catcgccagc ctgggcattc ccaaagacag cgtcatcaag
      181 tacgagacgg cggtcaaggc caacgggttt ctcgtgatgg ggcatggcac atcggaagaa
      241 atctcgaagg caaaggccgt gctcggcgcg ctcaatccct ctctgctgga cgtgcacgaa
      301 ggcttggcgc aagccagctc cgccgggcat gccatatcct aatacgaatt gaggcccccc
      361 gaacccgtgc agtctttggc tgcacgggtt tcttattggc ttctgctggt gctgtggagt
      421 cagattcttc atcgctcgga atcccgggac atcaaccggc gagccccttg cgaattgcct
      481 gcaattcccg tcgccggctt gcggtggatt ggggataggt catcttcagg gactcgagtg
      541 cgtccagtat gatgcgggag atgatcaacc gcgcgttctt cttgtcgtcg gccggcacga
      601 cgaaccaggg ggcgccgtgg gtgctggtcg cgccaaggca ttgttcgtag gccttcatgt
      661 actgcttcca gaattttcgc tccgcaatgt ccgccgcgct gaatttccag ttcttgtccg
      721 gatcgtcaat gcgcgcaagg aatcgtcggc gttgttcagc cttcgagaga tgcaggaaga
      781 acttgatgat gtgcgtgccg tttcgatcga gatgccgctc cagatcgaca atcgaccgat
      841 accgaccatg ccagaccgac tctttatcga gcaacgcatc cggcaagcct tcgccaagca
      901 gaatctccgg atggacgcgc acgatcagga cttcctcgta gtatgaccgg ttgaatatgc
      961 cgatttgccc gcgcgcgggt aaatcgcggg tggtgcgcca gagaaaatcg tgctccagtt
     1021 caatcggcgt gggatgcttg aagctgaaga cctggcagcc ctgcggattc acgcccgaca
     1081 tcacatgttt gatcgcgcca tccttgccgg ccgcgtccat ggcctggaaa atcagcaaaa
     1141 gcgcatggcg gttcgatgca tagaatagtt gctgcagcga actcaatcgt gccacatgcg
     1201 cggcgatcag ttcctgatag tgttccttcg agctgtacaa ggggtcgacc gtggtcggcc
     1261 acttgccaag gtttacgtca tctccctctt gcacgcagaa atcttccgat ctggttttca
     1321 tctcgagccc gtccatggtc atgtcagccg ccgccccgcc atcgcgcacc ctcgcatcga
     1381 caggaacggc atcgcgttct acgtaatatt caggcgagcg aagatgggaa tcttcagcgc
     1441 gtccagcaca agcgcaagga ctaccgttgc ggcaagcacc cccgccacca ccgcgacgga
     1501 tagcgacgtc attgcgatcc cgccaacggc aagaatcacg gcgatcacga cgtcggcaac
     1561 cgacgatgca atcagccaga gactgggacg ggtgccccac atgcgccggc gttgacgaat
     1621 cgcgtatagc gtcgcctggc tgccgaagac gaggaccacg aaggtcaagg tctggagcgc
     1681 gccggggccc agatccatct cgaacctgcc gattgccagc accccggtac agaacgccag
     1741 cagcagcacg ccgatgacca cgcccgcaat cgtcagattg ccgatgcgcc atgtattcgg
     1801 cgacgccgag gaatcgacgt tgtccgtcgt caatgacatg gcgagaaagt caccggcgat
     1861 catcaggatc accatcagca gcggcgtcag gatcgcgtgc cccgtcatga tgagaccgaa
     1921 caccagcaga agcgcggtcg tgatcttctt gatgatcgag ttcagcgtgt aggtctggat
     1981 gcgctggaat gtcacgcggc cctctttcac cgccgccacg atgccggtga gaccgggttc
     2041 ggtcagcacc atcacggcgg cggatttggc gacgtcggtc gccgtcgaca ccgcaatgcc
     2101 catttgcgcc tggcgcagcg cgggcgcgtc gttggcgccg tcgccgcaca tgccgaccac
     2161 atgcccgctt ttctggaagg ccttgacgag cttgtatttg tcctccggca gcacgccggc
     2221 gaacaccgtg aatgattccg gcttgacgct gtctggaatg ggccccggcg gacaggtcgc
     2281 gccgtcgaga ccgaccgcgt gggcgacgat gcccgcggtc gccggcgcgt ctcccgtcac
     2341 catcacggcg cgcacgccga gaccgtgcaa ctcggtgatg agcgcggccg agtcgtcgcg
     2401 cggcgggtcg ctcaacgcga ccatcccgac cagtttcagc gccgcgggtg cgcccgccgc
     2461 cacggcaagc acccgaaagc ccttcccctc aagctcattg gcctgcgctg ccgccgtcgg
     2521 caatggttcc gcacgccctg ttatgaccgc gaaagcgccc ttcacgattc gcagcgcgtt
     2581 gccggtcgaa tcggtcgcgc ttgcctcgga catcttcttg tctggatcga aggcggtgaa
     2641 tcgggtcagt ttcggcgcgt cggatgcgag atggtccgcg gcagccgcgc gaatcgccgc
     2701 atcgaccggg tctcccccgc cttcggcact cgccagcgcg gcgaacgtca gcacatgttg
     2761 gctatcgaag ccgggcatcg gttgcacggt cgtgacggtc agcgcgttgc gcgtcaatgt
     2821 gccggtcttg tcggtgcaca atatgtccat ggtgccagcc tcatcgacgg cggccaggcg
     2881 cgtagaaagt acgccgcgcc ctgccagggc gcgcgcgcca agcgcagccg acagcgtgaa
     2941 ggtggctggc aacgccaccg gaatcgacgc aagcacggcg gtcagcacga gcggcacgat
     3001 atccgcccac gacaggtgaa gatacaacgc acaggcaagc agcatcacga tgacgacgcc
     3061 gctgaagatg gccagattac gcacgaccag caataccgcc ttctgctgcg aacttgccgc
     3121 atgcgcggtg cggaccagct ccgcggtgcg gccgaagcgg gtatgaacgc cggtcgcggt
     3181 cacgacagcc accgcttcgc cgcggcggac cagcgcgccg gcataggtct gcacgccggc
     3241 agcgccttcg atcggcacgg attcgccggt caacatcgag tggtcgagca gcgcattacc
     3301 cgagacgagc ttcgcgtcgg ccgccaccac tgcgcccagc gatagcttga ctacgtcgcc
     3361 cgggaccaga tccgcggccg ggacggtcga ccacgctccg tcgcgccgca ccgaagcgtt
     3421 aagcgcaagc cgcgatttca gcgcggtcag ggtcgcctgt gcccggcttt cctgaaaaaa
     3481 tccgagcgcg gcgttgagca cgagtagtcc cgcaatgatt cctgcttcga caaattttcc
     3541 cagggcgcat tccaggacga cggcggcctc gagcatccac gggaccggcg cccagaattt
     3601 cccgagcgcc atgcgcaagg ggtgcgccga gacatccggt gtcgcgttcg gaccggattc
     3661 agccaatttg cggcgcgcct cggcactgct caggccactc tcggacggca cttccggcgg
     3721 gctcgtcgtc atggccgcgg gagtggtccc tgtcgtcgct ggtgtcatga ggtgttctct
     3781 ttctcggcca agttcttgct tttacgatag gccggcgtgc agagacaatc cgtacgaaag
     3841 cgtacggacg aagggaaatc ggaaaaatca gatgcggcgg cgggccgccg ccgcgcggct
     3901 acttgcgcac ttcgcggaac caggtgcgat gatgccgcca ggcccagccc ggcgtcacct
     3961 tgccgcgcac catggcgccc accgagccct tgacccagat cgccgcgtag atgtgcacga
     4021 tgatcgagca gatgatggca aagccgcaga gggcgtgcaa caccgccgcc gcgcggatca
     4081 cccagatggg gaagtacatc gagaagtacg cgcgccagat gacgatgccc gacagcagca
     4141 gcccggccat gcacaggatc agcacgtaga acagcatctt ctggccggcg ttgtacttgc
     4201 cgacctcggg caggttctct tcgcggttgg tcagcacgtc cttgatctgg ccgagccatt
     4261 gccggtcggc cgcggtcatg cagttgtact tgaacatgcg gatggcgaag atgaagaacg
     4321 agacgaacat caccacgccc acgaaggggt gcaggatgcg cgtccacggc ccgccgccga
     4381 acaggttggt cagccagaag aacgccgggt ggaacagcgc cagccccgac aaggccagca
     4441 gcacgaagca gatggcggtg atccagtggt tggtgcgctc gttggcggtg tagcgttcga
     4501 tgagcttgcc gccgtgattg gactcttgac tcatggtcac gcctcctgtt cggccttgcg
     4561 ctcgtcttct tcagacgtct cgttgggacc aatgcggatg tagtggaaga agccggccaa
     4621 ggccgagaag gccatgatgg ccacgcccag cggcttggcc acgcccttcc agaacgacac
     4681 catggggctg atgcgcgggt tttccggcag gccgtggtag agcatcggct tgtcggcatg
     4741 gtgcagcacg tacatcacgt gggtgccgcc cacgccctgc ggatcgtaca ggccggcctg
     4801 ctcgaagccg cgcgacttga ggtcctcgat gcgctcggcc gcgtgctggt gcatgtcttc
     4861 cttggtgccg aaggtgatgg cgccggtggg acaggtcttc acgcaggccg gctcctggcc
     4921 gaccgcgacg cggtcggagc agagcgtgca cttgtaggcc ttgctgtcct tcttggagat
     4981 gcgcggcacg ttgaacgggc agccggtgac gcaatagccg cagccgatgc agttctcttc
     5041 gtggaagtcc acgatgccgt tggtgtactg cacgatggcg cccggcgaag gacaggcctt
     5101 caggcagccc gggtcttcgc agtgcatgca gccgtccttg cggatcagcc actccaggtt
     5161 gcccgtctcc tcgttctcgt attcggagaa acgcatcacc gtccacgagt gctcggaaag
     5221 atcggccggg ttgtcgtaga cgcccacgtt ggtgccgatc tcgtcgcgca ggtcgttcca
     5281 ttccatgcag gcggactggc aggccttgca gccgatgcac ttggagacgt cgatcagctt
     5341 ggccacggtg ccggtggaag gctcgcgcac gccgggaggc ggcgtggtcg tcgacgagcg
     5401 ccgcttgatg tcaagggatt gcagggacat gttggcgctc ctatgccttc tcgaccttca
     5461 cgaggaagga cttgaattcc ggcgtctggc tgttgccgtc gcccacgaac ggggtcagcg
     5521 tgttggcgag aaagcccggt ttggcgatcc ccacgaaacc ccaatggatg ggaatgccca
     5581 cgtgatggat cttcttgcca tcgacgtcga gcgccttgat ccgcttggtg accagcgcca
     5641 ccgccttgat gtagccgcgg ttggacgaga ccttcacctg cgaaccgttg gcgatgccca
     5701 gctcctgcgc cagcgactcg ccgatctcga cgaactgctg cggctgcagg atcgagttga
     5761 gctcgacgtt cttggtccag tagtggaagt gctcggtcag ccggtaggtg gtgcccacgt
     5821 acgggaactg ctcgaccttg cccatggcct ccaggtcgcc cttgaacacg cgggcggccg
     5881 ggttgttggt ggccgtcttg ctctggggat agaccgggtt gtagcccagc ggggtttcga
     5941 agggttcgta gtgcgtggga aaggggcctt cggccatgcc cgcgcgggcg aagaagcgcg
     6001 ccacgccctc ggggttcatg atgaaggggc ccatgccgtc ggcgggcttg gacgtcgcca
     6061 cgaagtcggg gatgtcggcc ccgccccagc ccttgccgtt ccaggcaatc aaggcgcgct
     6121 tggggttgta gggcttgcct tccaggtcgc acgaggcccg gttgtagagc acccggcggt
     6181 tggccggcca ggcccaggcc cagttcagcg tctggcccac gccggtgggg tcgctgttgt
     6241 cgcggttggc catctggttg ccggcctgcg tccaggcgcc gcagaagatc cagcagcccg
     6301 acagcgtggt gccgtcgtcg cgcagctcgg caaaacccga gagctgctcg cctgccttgc
     6361 gcacgaccgc gccgctcgcg tcggtgaggt cgaccagcgc gcggccggag tactccttgg
     6421 ccagctcgac ggccgtgggg ctttccgggt tggaataggg ccacgacagg ttgacgatgg
     6481 ggtcggggaa cttgccgccg tccttgcggt acatgtcgcg caggcgcagg aacaggcccg
     6541 acatgatctc gaggtcgctc ctggcctcgc ccggggcctc ggcgcccttc cagtgccatt
     6601 gcagccagcg gcccgaattc accagcgcgc cctcttcctc ggcgaagcag gtggtgggca
     6661 ggcggaagac ctcggtctgg atcttgctgg agtcgacgtc gttgtgctcg ccgtagttct
     6721 tccagaactc ggaggtctcc gtgttcagcg ggtccatgac gaccatgaac ttgagcttgg
     6781 cgaaggccga cagcagcttg gccttgtccg gcgcggccgc cagcgggttg aagccctggc
     6841 agaggtagcc gttgaccttg ccctggttca tcagctcgta cgtctgcagc atgtcgtaca
     6901 tcttgtcgag cttgggcagg tagtcgtagg cccagttgtt ctcggccgtc gcggcgctgc
     6961 cccaccaggc cttcatgaag ctgacgtgga attttttata gttctgccag tagctcaact
     7021 ggcccgggcg cagcggcttg gaggcgcgcg cgtcgatgta ctggccgaag tcctgctcgg
     7081 gctcgttggg cagcgtcatg tagccgggca gcaggttcga catcaggccg aggtcggtca
     7141 ggccctggat gttcgagtgg ccgcgcagcg cgttcatgcc gccgcccgcg atgccgatat
     7201 tgcccagcaa cagctgcagc atggcgccgg tgcggatgat ctgcgagccg atggagtgct
     7261 gcgtccagcc cagcgcgtac aggatggtgg ccgcgcggcc cggggtggcc gtggaaccga
     7321 gcatctcgca caccttcagg aacttgtccc tgggcgtgcc gcagacgcgt tcgaccatct
     7381 cgggcgtgta gcggtcgtag tgctgcttga gcagctggta gaccgtgcgc ggatgctgca
     7441 gcgtggggtc ggtcttcacg aagccgtcgt cgccgcgctc gtagtcccac gaacccttgg
     7501 cgtaggtccg cttctcggcg tcgtagccgg agaacacgcc ttcgtcgaag gcgtagtctt
     7561 cacggacgat gaacgaaaaa tcggtgtagt tcttgacgta ctcgtggtgg atctggtctt
     7621 tttcgagcag atagcggatc gcgccgccca ggaacacgat gtccgagccg gtacggatgg
     7681 gcgcatagaa atccgccacg gatgccgagc gcgtaaagcg cggatccacg acgatcagct
     7741 tggccttgtt gtgggccttg gcctcggtca cccacttgaa gccgcagggg tgcgcctcgg
     7801 cggcattgcc gcccatgatc aaaaccacgt ccgcgttctt gatgtcgacc caatggttcg
     7861 tcatcgctcc acggccaaac gtcggggcaa gacctgccac cgtcgggccg tgtcagacac
     7921 gggcttggtt gtcgaatgcc agaacgccca ggctgcggac gactttatgg gtgaggtagc
     7981 cgacttcgtt gctgctcgcc gaggcggcca gcacgccggt ggtgagccag cggttgaccg
     8041 tcttgccgtc gtcgctcttt tcgacgaagt tggcgtcgcg gtcggccttg agcagcttgg
     8101 cgatgcggtc cagcgcctcg tcccaggaca tgcgccgcca ggtgtcggag cccggggcgc
     8161 ggtactcggg gtacttgagc cggttggggc tgtggacgaa gtcgatcagc gaagcgccct
     8221 tggggcacag cgtgccgcga ttgaccggat ggtcggggtc gccttcgatg tggatgatgc
     8281 tcgcctcggc gttcttggcg cggtcgccca gaccgtacat caagatgccg caagcgacgg
     8341 aacaatatgg acaggtgttg cgcgtttcgg tcatgcgcga cagcttgtac tgccgcacct
     8401 cggccagcgc agtaccgggg gcagccccca gcaaggccag gctcgagccc gccaacgttg
     8461 cccctgtcac cttgaagaac tggcggcggt tcatgttgac catgaagcac tcccttcgca
     8521 gtggataatg ggtatgcgtg caggccctgc cccagcaaga ctgcgcgacg tctctgctta
     8581 cgccggcagt atagggcgac cctgccgccg atagggggaa atttgtgtcc gcggcgcatc
     8641 attgggaata ttgcggcgca acatcccatc gacggcatcc cggccagccc ttacagtaaa
     8701 ggccttctcc tctgcccgca ccctgcccat ggacacaccc agccaccagt tgtcgatgac
     8761 catcctcatg acgcccgaca cggcgaactt ctcgggcaat gtgcacgggg gcaccatcct
     8821 caagctgctc gaccaggcgg ccttcgcctg cgccagccgc tatgcgggga cctatgtggt
     8881 gaccctgtcg gtggaccagg tggtgtttcg ccagcccatc cacgtgggcg agctggtgac
     8941 cttcctcgca tcggtcaact acaccggccg cacttcgatg gagatcggca tcaaggtgat
     9001 cgccgaggac atccgcaaga aaatcgcgcg ccacgccaac agctgctatt tcacgatggt
     9061 ggcggtcgac gaccagggcc agcccatctc ggtgcccccg ctcgagcccg gcgacctgga
     9121 agaggcgcag cgcttcgaag gcgccaagca ccggcgggcc ctgcgcgagg aaatggaaaa
     9181 gcgccaccag gacatcaagc agaccatcca ggactgcaac ggcaagcccg gctaggcccg
     9241 gctgccaggc aggcagggtt tccccttgcg cgcatatagt tgcgtgcgca actaaaaacc
     9301 ttttatagtc gctccagaca catcaataac aacatctcct ggcccgctcg actcgaccgg
     9361 ccggcgagtt ctggagacat gggaccatgc aagcgcccgc ttccctgccc gaccgcgtac
     9421 ccgtcctcat cgccggcggc ggcccggtcg gcctgtccct ggcggccctg ctggccgggc
     9481 agggcatcgc cagcctcacc atcgaggccg acgccgagta ctgctccggc agccgcgcca
     9541 tctgcatgtc gcgccgctcg caggagatcc tgggctgggt gggcgccgat gtccccctgg
     9601 tcgagaaagg cctgtcctgg accggcggac gcagctactt ccgccacgag gaagtgctgc
     9661 acttcgagat gcccagcgat ccgctgcagc gctacgcgcc catggtgaac atccagcagt
     9721 actacatcga ggagtactcg catcgcgcca tgcagcgcca cggcggcctg gcgcaggtgg
     9781 cctgggccag ccgggtgacc ggcctgaagg acctgggcga cgcggtggag gtcgccgtcg
     9841 aggccggcgg ccagcgccgc accgtgcgcg ccgactgggt cgtggcctgc gacggcggcc
     9901 gcagcaccgt gcgcgaactg ctcgacttgc acttgcgggg catgcagtac gaaggccgct
     9961 acgtgatcgt cgacatcgag caggacacgc ggcgccccgt cgaacgcctg gcctggttcg
    10021 acccgccgtc gaaccccggc tcgaccatcc tcatgcaccg ccagcccgac aatgtctggc
    10081 gcatcgacta ccaggtcgac gacgatgccg acgccgccga ggccatcaag cccgagaacg
    10141 tgctgccgcg cgtgcagagc cacctcgaca tgatcggcga aaccgcgccc tggaagccgc
    10201 tgtggatctc gatctacaac gccaagtgcc tgacgctgga cagctaccgc cacggccgcg
    10261 tgctgttcgc cggcgacgcg ggccacctgg tgcccatctt cggcgtgcgc ggcctgaact
    10321 cgggcctgga cgatgccggc aacctggcct ggaaactggc ctgggtgctg cgcggcgcgg
    10381 cgcccgacag cctgctcgac acctacagcc aggagcgcgt ctacgccacc cggcagaaca
    10441 tcgaatacgg cgccaagagc accgagttca tggccccgcc ggactacggc tttcgcctgc
    10501 tgcgcgacgc cacgctgcgc ctgtcgctgg tcgagcccag cgtgagcgag ctcatcaacc
    10561 cgcgccagtc ctcgcccatc acctacgccg gttccccgct gaaccagccc gacggcgcgc
    10621 cggcctgcgg cgccgccgcc gcgccgggcc agcccgcccc cgaggcgctg ctcgcccggg
    10681 acggcaggcc cgtccacctc accagcctgt tcggccgcgg cttcgtcgcc ctggtcaacg
    10741 gcgaggcgcc cgaactgagc ctgctgcaat ccctgcaagc gaagacccgc ggcacgcccg
    10801 cgcccttcga ggtggtggag atcggggcag gcgcctacgc cgacccgcac gggcagatcc
    10861 gcgagggcta cggcgcccaa cccggcctcg tgtacctgat ccgccccgac ggctacgtca
    10921 tgggccgctg ggccgatgcc ggcgccgccg ccagcgcgat gcgcgccctt gccccctttc
    10981 acgcctgacc cgcgcaggag ccgaccatga acagcaccga tctcgacacc gcctacgccg
    11041 cgctggccca tgccctgacc cgcgtgggcg aggcccgcgc gccgctgttt cttgccatgc
    11101 tgtgcctgtc gctgatgagc cgcaatccgg acgtcggcca ggtattggcg ctgatggaac
    11161 aggccgaggc caagcagtcg ccataaaaat tgcgggcgcg cgcaacctgc tttcgggccg
    11221 gttatgcttg gcgcaaaaaa agccatatcc gcagcgccac aaccatacca atccggagac
    11281 aaacccatgc gtatccttgc ccatgcagcc gcggcgcttt tgctcgccgc cggcctgacg
    11341 ccggcccacg cggccgatga ttaccccgcg gcctacccca tccactgggt cgtgccctac
    11401 gcggccggcg gcggttcgga catcgtggcc cgcaacctgc aggaaggcat gtccaaggtc
    11461 ctgagccaga ccatcatcat cgacaacaag ccgggcgcgg ccacggccat cggcgccgaa
    11521 tacgtggccc acgccaagcc cgacggctac accatcctgt cgggcgacac cgccacgctg
    11581 gcggccaatc cgccgctcta cccccgcctg gcctacgacc cgcagcgcga tttcgccccc
    11641 gtgggcctga tggcgcgctt caacatgatc ctggtggtga acccctcggt gccggcgcac
    11701 ggcatgaagg aattcatggc gtgggccagg gcgcagaagg acggcgtgag ctacggcacg
    11761 ccgggcgccg gcagcccgca ccacctggcc accgaattgt tccgcgcgcg caccgggctg
    11821 aacctcaagc acgtgcccta ccgcggcgcg gcgccagccg tgcaggacgt gatgagcggc
    11881 caggttccca tgatgttcgt cgactcggcg tccggccagc agtacatcgc cagcggcaag
    11941 ctgcgcgccc tggccgtggc cagcaaggac cgcctgcccg gcatgcccga cgtgcccacg
    12001 ctgatcgaat ccggcctgcc cgacttcgag gcctacgcct ggcagggcct ggtcgttccc
    12061 aagggcacgc cccagccggt catcgacaag ctcaatgccg ccctgaacga aacgctgaaa
    12121 tcgcccacgg tcatcgagaa gttcaaggcc atgggcctgg aagtcatccc cagcacgccc
    12181 cgggcgctcg acgactacgc ccgcgccgaa cgagccaagt ggacccaggt gatccaggac
    12241 gcccacatca cgatcgagta gcatcggttg cccatgcccg ccgaacgccc gtccgatacg
    12301 gaaccgctgc ccgacctgga gcggttcctc acctaccggt tgcacgtcgt caacaagctg
    12361 tccgaccgcg acaccaaccg ggcctacctg gacaagctcg aattgccctt gggagaggcg
    12421 cgctgcctgg cggccatcgg ccgcttcgcc cccttgtcgg tgaaagacct ggcgcgcgcc
    12481 gccaacctcg acaagggcca ggccagccgc tcggcgaaat ccctggccga gcgcggcctg
    12541 gtcgacaagg ccgccagcgc caccgacgcg cgcggcgtgc tgctcacgct cacgccggcg
    12601 ggcgcggaca tgtatgaacg cgtcatccgc ctgatcgagg cgcgcaaccg ggagatcttc
    12661 ggctgcctga gccccgccga acgggagcac ctgggccgcg ccctggaccg catcgcccgc
    12721 cacctggccg acggacagga ccaggacggc tgaaccgcaa gaaccggagt gttcgcccgg
    12781 ggtatcgtgg ctaaagtaag cgccatggac acccctctcg ccgacatcat gccgaacgcc
    12841 actcagcacg ccgccctggt cgccgcggcc tgtcgcctca tcgaagccgc cgacgcgccg
    12901 cccagccttg ccgagctggc ggcccgcgtg ggcttgagcc cgcaccactt ccatcgcctc
    12961 ttcaaggccg agaccgggct cacgcccaag gcctatgcgg ccgcccacca ggcacgccgg
    13021 ctgcgcgccg ggctggccgg ccagggcacg atcaccgacg ccatcttcga ggccggctac
    13081 aacgccaaca gccgtttcta cgagcaggcc gaccagcggc tgggcatgcg accgcgcgac
    13141 tatcgcgcgg gcggcgccaa taccgacatc catttcgccg tcggccaatg cagcctgggc
    13201 gccatcctgg tggccggcag cccgcgcggc atttgcgcca tcttgctgga cgacgacccc
    13261 gacgccctgg tgcgcgccct gcaggatcaa ttcccgcgcg cgcgcctgat cggcgcggac
    13321 cgggacttcg agcaatgggt ggcccaggtg gtggggctgg tggagacgcc gtcggtgggc
    13381 ctgaacctgc cgctggacgt gcgcggcacg gccttccagg aacgcgtctg gcgcgccctg
    13441 cgcgacattc cgcccgggac gaccgccacc tacgccgatg tcgcgcggcg catcggcatg
    13501 cccagcgccg tgcgcgccgt ggccggcgcc tgcgccgcca acaagctggc ggtggccatc
    13561 ccctgccacc gcgtggtgcg cagcgacggc agcctgtcgg gctaccactg gggcgtggcg
    13621 cgcaagcgcg cgctgctgga ccgcgaggcc gaacaggccg cctgaaagcc cccggcgcgg
    13681 cacgctcccc ctgccgggag tgaggtcatg acgagaatgc ggccgtcccg gctggtataa
    13741 cgggcgggtc attccgctac tcgattccgc cacgcccatt ccccatgaaa gccatcgcct
    13801 atacccagca cggcctgccc gccgacgacc ccaaggccct gatcgacctg gacctgccca
    13861 cccccgaacc cgggccacgc gacctgctgg tggccgtgcg cgccatttca gtcaacccgg
    13921 tcgacaccaa gatccgcatg ggcgtcgcca ccgaagcgcc gcgcatcctg ggctgggacg
    13981 ccgtcggcac cgtcaaggcc gtcggcagcg cggtcagcct gttcaagccg ggcgacgagg
    14041 tctattacgc cggcacgctg ctgcgcccgg gcaccaatgc cgagttcagc ctggtcgacg
    14101 aacgcctggc gggcgcgaag cccaagtcgc tgtccgatgc ccaggccgcg gccctgccgc
    14161 tgaccacgct cactgcctgg gaattgctgt tcgaccgcct gggcgtgccc gaaggcggcg
    14221 gcgcgggcca gagcctgctg atcgtcggcg ccggcggcgg cgtgggctcc atcctcgtcc
    14281 agctggcgcg caagctgacc ggcctcaccg tgatcggcac ggcctcgcgc gccgagaccc
    14341 gccagtgggt ccaggacctg ggcgcccatc acgtcatcga ccacggcaag ccgctggccg
    14401 gggaactggc ccgcgtcggc gtgccgcagg tcgacctggt cgccggcctg acccacaccg
    14461 aggagcacta cgcccagatc gtcgaatcgc tggcgccgca gggcaagctg gccttgatcg
    14521 acgacccggc cgtgctcgac gccgtgccgc tcaagcgcaa gtcgatctcg ctgcactggg
    14581 agctgatgtt cacgcgctcg ctgtacgaga cgcccgacat ggtgcgccag cacgagatcc
    14641 tcgaacgcgc cgcccagctg atcgacagcg gcgtgctcaa gaccacgctg ggcgatcact
    14701 acggtaagat caacgccgcc aatctgcgcc gcgcgcacgc catgctccag agccacaagg
    14761 cccgcggcaa aatcgtcctc gaaggattct aagcatgccc tacgtacgtg ttgaaatggt
    14821 cgaaggccgc agcgacgagc aaaaagccaa gctcgcgcaa gccatcaccg ccgccatggt
    14881 cgagcacgcc ggcgctcccg ccgacagcgt cttcgtcgtc ctggaagacg tcaagaagaa
    14941 caactgggcg atcggcggca cgctgatctc gcagcgcaag aaataagact cccgcgttgc
    15001 gcgccttgcc gtcccgaaag ggcggcaagg cagcccgaac tcaaggcagc agctgtatgg
    15061 ccggcagcgc cgcgtcgcaa ttgagcagca ggaccttctt gatcttgatg cgcaggcctt
    15121 gcggcgtgct ggcgaccagg tgctcggccg tggcggccag cacctgctgg gcatcgccct
    15181 gcgtctcgac gtagaggaag ggcgtgctca aggcatagcg gccgcccgcc tcgtcgcggc
    15241 tcgtcacctc gggcgcctgc agcacgtgca ggcagcgcac cgccgggtgc agcgagtgcg
    15301 ccttggggtc tttcatgcgg tccaggcgca ggcgcagcag catcttgtct tcatacatca
    15361 gcgcgggctc ggactcgccg ctctgctgtc cgggcgtgag cggcatccag tacaggccgt
    15421 cctcgtcgaa gagggcgtac cactcctcga aacggccttg atcgatgagc cgggcttcct
    15481 ggtagaccag ccgggtcagt tccgcatccg cgggcgcttg cgtcatggcg tgtcctctcg
    15541 cgtcttccgg tcaggccgga tccatcaggc tcaaccagcc gcgatactgg ttgcgcatca
    15601 tgatttcgtt gttgccgttt tccagcgcca ggctgggcgt ctgcgtttcc ccggcctggt
    15661 ggccgcggtg caggctcacc cattcgttgc cgtcgctgcc caggcctatc tgctggctct
    15721 cgaagagatg cacgtcatcg tgcgccacca ccgacatggg cgaaaacacc aggcgcacat
    15781 aggaagccac ccgctggcgc agccgctgcg gcgcgccctt gggcaccatg gcccaggcct
    15841 cgatcagcgt gcggtccggg cccagcggcc gcagcacgcg catcagcgat ggcgaggtct
    15901 tgaacgccgc gctggggtag aacaccgtgt tttgcggcga gaaggacagg agctccctgg
    15961 cctcttcctc gccgtacagg ccggcaagtt cggcttcgta ctcgggcaac ggcgcatagc
    16021 cggtatggat gctggccttg gtacccagca cgctgtgccc gttgggcagc accttggcgc
    16081 ccatgtcgct atagaagctg tagcccgcgc cgaaaggcag cagctgctcc atggcgagcg
    16141 aagtgcgggc atcgccctcg ggcagcgact tgccgacctt gcccgccgcg gtggcggccg
    16201 actcgtgcgt ggagatggga tgcaccgtgt cgttgatatt ctcgaggtac atcttccagt
    16261 tgcagcgcat cacattgcgc aggggcgcgc ccaccacctc gagctcgccc accggcgaac
    16321 gcgccaccat attgtcgagg gtggccagca cggccgggga gaagtagctg tcgaagtccg
    16381 ggcccgccgc gctgatgcgc gtgaacacga agcccttgta gttgcgcacg ccgcccggcg
    16441 cggccatgcc ctggcccgaa gggctgtccg acaggccggt accctcgtag tcgttcttca
    16501 gcgggatcga catcagcttg ccgtcgagct tgtacgtcca ggcgtggtag gggcagcgca
    16561 ggaacttgcc ggcgttgccc tgccgttcgt gcagcaagcg gctgcccttg tgggcgcagc
    16621 ggttgaacag caccgccacc ttgccgtcgg cctggcgcag catgacgagc gactgcccgg
    16681 cgatctcggc cgtgtagtag tcgccggcat tgggcaccag gctgtcgtgg cccaggaata
    16741 cccaggcgcg cttccacagc cgttcctgct cgagccgata gacgcgctcg ctgatgtaga
    16801 cgtcgcgatg gatgcggtcg ggctcgacca gatcggccac cgactcgtcg ccggcatccg
    16861 gcggcagcat gctcagggac aatgacatga tgaacctcgt gtttccagtc aggccgggac
    16921 gggcgacagc gcgggcggca agacgggggg ccaggcggcg tcccccgttt ccggcggcac
    16981 cgccgcgatc agggccttgg tgtagtcgtg ccgcggcgaa gtgaagacct gctcggtctc
    17041 gccggcctcg accaccacgc cgcgatgcat gaccatcacc cggttgcaca ggtagcgcac
    17101 cacggcgagg tcgtgcgaga tgaacaccag gccgatgccc agctcgcgat tcaggcgcag
    17161 cagcatgttg aggatgagcg cctgcaccga gacgtcgagc cccgacacga tctcgtcggc
    17221 caccagcatg cgcggcgtca ggcacagggc gcgggcgatg ttgacgcgct gcttctgtcc
    17281 gcccgaaagc tgcgacggca ggcggccgag cgaactgggc gacaggccca cggcctgcat
    17341 caggcgccgc gcggcggcct ggcggtcggc cgcctgctcg gtcccggcgc cgtcggcctc
    17401 gaggatctgc gtgaccagat cggccacccg gcggcgcgga ttgagcgccg aatcggggtt
    17461 ctggaacacc atctgcacgc atttgcgtat gtcacgcgcc tgctcggcat tggccgcggc
    17521 gcggtcctgg ccatcgatca ggatgctgcc ggccgtcggc ggcaccaggc ccatcaggag
    17581 gcgcgccatg gacgtcttgc cgctgccgct ctcgcccacg atgccgagga actcgcccgg
    17641 cgccacggtg aacgacaccg gctgcaccac atggatgggc tcggccttct tccagccact
    17701 ggcgcggccg ggatagctga gcgacacatt gcggaattcc accagcgggc gggcctggcc
    17761 gtcgccggcc ggcaggctgg gcgccggatc ggcggcctgg cggtcgatgg cggggatgag
    17821 ctccaggcag cgggccaggc ggcccgcgcc gcgcggccgc agcgccggcg gccggcgctc
    17881 gcacccgggc tggcgcacgg cgcagcgcga gtagtagcgg cagccggcaa tgtcggggaa
    17941 gtcgcccacg gccggcatga agtcgggcag cgaaggcagc gcgacgcgcc cgccctgcaa
    18001 ggccggcgtg gcgctcttca ggctcagcgt atagggatgg acgggatggt cgagcacctg
    18061 cgcggccgag ccggactcga ccacctcgcc ggcatacaac acggccacct ggtcgcacac
    18121 gtgcgcggcc aggcgcaggt cgtgcgtaat gaggatgacg gccgtgccgt ggcgctgctg
    18181 ctgttcgcgc agcacgcgca tgatctgcgc ctgcgtcatc acgtccagcg ccgtggtggg
    18241 ctcgtcggcc acgatcagcg ccggctcgcc gcagaaggcc atggcgatga ggatgcgctg
    18301 gcactgtccg cccgagagct ggtgcgggta gcgcgccagg atggcctcgc cattgggcaa
    18361 gcccacctcg gccagccggt cgagcgccat gcggcgccat tgcgccttgg gcgcgcccac
    18421 gtgcgccagg tgttcgaaga tctgctggcg caccgtcagc acggggttca ggcccgacag
    18481 cggttcctgc ggaatgaagg cgatgcgccg gcccagcagg gcgcggtgct gcgcgcgcgg
    18541 caaggccatc aggtcctggc cttcgaagcg cacgaagcca tgggtcaccg cgaagccggg
    18601 cggaatctcg ccggcgatca tgcgccctat catgctcttg cccgcgcccg attcgcccac
    18661 caggcccagc atggcgccgg gctcgacgtg cagcgcgatg tcgcgcagca cgtcggcgcg
    18721 cttgcccgcc acgttcagcg acagggtcag ttcgtagaat tcgacggcag ccatgtctta
    18781 cacccgcgac gtgttggcgc gcgggtccag cgcctggcgc aggccgtcgg acagcaggtt
    18841 gaagcccagc accgtgacca cgatgccgca gaccggcgga atcaggttcc agatgttctc
    18901 ctgcatgtag ccgcgcgcgt cggcgatcat cacgccccag gccaccgtgc tggccggcac
    18961 cgacaggccg acgaagctca ggatggcctc gaccacgatg gccacgccca tctccaggct
    19021 gaacagcgtg atgatgagcg gcaccaggcc gggcagcatc tcgcgcagca tgatgcgcag
    19081 gtgcgagaag cccatcagcc gcgaggccac cacgtagtcg cggcggcgta tcaccagggc
    19141 ctcggcgcgc accacgcggc aaaagcgcgt ccagtcgacc agcaccacgg acaggatcac
    19201 attgcccacg ccgggcccca ggcccaccat caggatgagc gacagcacca ccggcgggaa
    19261 cgacatccac aggtcgacca ggtagctgat gccgcggtcg accttgccgc cgaaatacga
    19321 gcccagcagg gccagcgcgc agcccagcag catggtgccg accgccgagc acaggcccac
    19381 ccacagcgcg acgcgcgagc cgaagatcag gcgcgacagc acgcagcggc ccaggctgtc
    19441 ggtgcccagc gggtactggg cggtgccgtc ggccatccag gccggcggca gcaagggcga
    19501 gagcaagtcc tgcgacagcg ggtcgttggg cgcgatccag ggcgcgaaga ccgccaccag
    19561 caccagcagc agcacgatgg cgccgcctat caaggccttg ggcgagcggc ccagggaacg
    19621 gagcagcggg cgccagcggc tgcccgattg catggcgaca gaagacatca ggcgtccctc
    19681 agccggggat tgaccaccgt gtacaggcag tcgatgcagg tgttgatgat caggatgatg
    19741 acgcagaaca ccagggcgat gccctggatc agcggcaggt cgtggttgcg cacggcctgc
    19801 accatcaggt tgcccaggcc ggggaaggaa aagatcatct cgatgagcag cgtgccgccg
    19861 aacaggaagc cgaactgcac gccgatgagc gtgagcgtgg gtagcgccgc gttcttgagc
    19921 atgtgccgca gcacgatgcg cgtgtcggac tggccgcgca gccgcgccac catggtgtat
    19981 tgctcgcccg ccgcctcgag caggctggcg cgcagcacgc ggatgatcag cggggcgaag
    20041 cccagcgcca gcgccagcgc cggcagcacc aggtgcgaga acgcgttgcc ccaggccctg
    20101 aaatcgccgc gcagcaggaa gtcgatcagc acgaagcccg tggcgccggg cggcgcgacg
    20161 tcggcgccga agcgccccga aaacggcagc agtggccaca tcacgcccag gcccagcatc
    20221 aggaagatgc cccacaggaa ggacggcacc gagatcagca gcaccacgaa gccgtcggcc
    20281 agcagctcgc cgcgccgccg gtgcagcgca tacagcagca ggccgccggg gatgctgatg
    20341 accagcgaaa acaccagcga cacgatggac agctcgagcg tggccggcag gctggtggcg
    20401 atcatccggg tgacctcgac gcccgaggtg atcgaacgtc ccaggtcgcc gtgcagggcg
    20461 tggacgagcc agatgccata ttgcaccggc agggaccggt ccagccccag ggccgcgcgc
    20521 agcgtgtcga tctcggcctg cgtggcggtg ggcggcagca tcatggccag cgggtcggcg
    20581 ggcagcagcc gcagcaccac gaacaccacc accgacagcg cgaagatcat cgggatcgcg
    20641 acagccacgc gcgcgagcgc ctggcgggca aggacggcaa gcatggctta ctttttccag
    20701 ctgtactctt gaggcatgat gtagccggtc tgataggtgc tcaggttcag cgacttcttg
    20761 taggcgatgg tcgacacgct ctgcagcagc gggatgctcc aggcgttctc ggtggccttc
    20821 ttctccacgt cgcgatagcc ggcgatgcgc tggtcctggc ccatcgcggc gagctgcttg
    20881 aaggtgtcgg ccagctcggg ctgcttccag gccgagaacg gcaggctggg gttgaggatc
    20941 atgcccgaga agatttccgg gtcgccggtg gcgttgtccc agctgtagag catggggccg
    21001 ggcagcttgc cggagtggct gagatccagg tacttggcca gggtcacttc ttccaggtcg
    21061 gccttgatgc cgatctgcga ccacatctgc acgatggcgc gggccatctc gtagtcgccc
    21121 ggcaccgagc cgttggccga caggaaatgg atttccagcg gcttgccggg gccgtagccg
    21181 gcctgggcca gctcggcggc ggcggccttc ttgtcgaact tgacgtcgaa gccgtcgaca
    21241 taggcgggcg tgcccggcag ggccagcacc gagatcggct tggccgagtt ggcgtagaaa
    21301 gcgcgcgaca gcgcctgctt gtcgatggcc agctgcatgg cctggcgcac gtgctcgttc
    21361 tgcagttcgg gcacgtagct gggcatctgc agcatgaaga cctgggtgta cgggtacacc
    21421 ttggcggcga tggcgtcggt cttggccagg cggttcacct cgcgcacggg gatctgcacg
    21481 gccacgtcgg cgcggcccga ttccagcatg gccacgcggg ccgaggtgtc gggcacgaaa
    21541 tcgaagacca cgcgcttgat ggcaggcttg gggccccagt agccgtcata ggcctcgagc
    21601 acggcgcgcg agccgcgctc atagctcacc agcttgtagg ggccggcgcc gatgggatgt
    21661 tcgaggaact ggtcgacgcc gaccttctcg atataggcct tgggcaggat gtaggccgtc
    21721 aggaagccca gccagttggg cgccgtgggc atcgggttct tgaagttcag caccgcctcg
    21781 gtgggcgact tgatgtcgat gctggcgaac ttgctggtga aggtgctgcc gatcaccagc
    21841 ttcttgtcgg ccaggggccg gtcgaggaag ctgtacttga agtcttcggt ggtgagcttg
    21901 ctgccgtcct ggaaggtgac gtcgtcgcgc agcacgatgt ccaggctggt gttcttgtcg
    21961 tcgagccact tccaggactt gatcaggcgc ggcgtgacct cgagcttgtc gttgatcatc
    22021 agcggggagt cgaacacggc cttgtagagg gactggccgg cggggaaggt gcccgcggtg
    22081 gggtcccagg tgggcaggtc gaccgggtag gcgatggtca gctcatcgcc ggcggcgctg
    22141 gcggtgggcg ccgcggccgc gcaacccagg gccaccaggc aggaagcaag cgctcggcgg
    22201 gcgggcgcgg tcagtaaacg ggtactcatg tgcagcctcc aaaatggcca gttccggggc
    22261 ggccccggca gtgggaagtc cgaatgcaag aatgtggaac agcaatgaaa accgtatatt
    22321 gctgaagaca catatgccaa ttactttaag cataaagtct ttttgtatat ttaaaaacaa
    22381 tggtttactc ttatcaagaa caatatattg tttaagtgaa aacccttatt acccgacatc
    22441 cggctgacag gtaggcttgg ccttggtcac ggcgaaccct atccaagagg agcaccatgc
    22501 aggcgcccgg cagtcctggc aagaaaccca atctgcgcga acgcgtctac cagatgctgc
    22561 gggaacgcat ccagatgggg ctgatcggac acaacgaccg cctggtcgac cacgacatcg
    22621 cgcacgacat gcacatctcg cgcatgcccg tgcgcgaggc cctgatgcag ctcaagagcg
    22681 agggcgtgct ggaaggcacg gcgcgcggct tcatcctgcg gcgccattcg ctgcagcaga
    22741 tcaacgagat cttcgaaatc cgcatcctgc tcgagccgca tgccgccggc ctggccacgg
    22801 ccaacgccag cccggccttc ctggcccgga tgaaggccgc cctcgacgcc gccgagcagg
    22861 cctcgcgccg cggcgactgg gagtccttca tgcgcatcaa cgccgactac cggaccgcct
    22921 ggatcgccat ggtgcccaac cgggaaatgg ccgacaccat ctcccggttc atcgaccacg
    22981 tgcaggtcgt gcgcctgatg accatgcagg acgacaccgt ccgcaacatc atcctggacg
    23041 gcatgcgcgg cctgtacgag gccttcctgt cgggcgacgc caacctggtg cgcgaacgca
    23101 tgaccaccca ctgccggacc gcgtccgcct cgtactacca gtgctaccag cggctctatg
    23161 acaccgggcc gcccccgacg aactgaccct ttttcatgaa acaccctcga cggtgctaga
    23221 ctttaaaacc cgcaggcccc tttacggaat aaagggccgg gccgcccgtg cggacggctg
    23281 gactgctctg gtttcataca agaccacgaa tcaaggctga acgaatgtct accacgtcga
    23341 agatcatcta caccctcacc gacgaagcac ccgccctcgc gacgtactcg ctgcttccca
    23401 tcgtgcaggc ctacgccggc cccgccggca ttgccgtcga aacccgcgac atctcgctgt
    23461 cgggccgcat catcgccacc ttccccgaag tgctgaccga tgcgcagaag atttccgacg
    23521 agctggccta cctgggccag ctcgccgcca agcccgaagc caacatcatc aagctgccca
    23581 acatcagcgc ctcgatcccg cagctgaagg ccgcgatcaa ggaactgcag ggcaagggct
    23641 acaagctccc cgagtacccc gacgagccca agaacgacgc cgagcaggac gtcaagaccc
    23701 gctacgacaa gatcaagggc agcgccgtga accccgtgct gcgcgaaggc aactccgacc
    23761 gccgcgcgcc cctgtcggtc aagaactacg cccgcaagca tccccacaag atgggcgcct
    23821 gggcggccga ctccaaggcc cacgtggccg acatgccgag cggcgacttc tacggcagcg
    23881 agaagtcggc cctgatcgcc gccgaaggcg ccgtcaagat caccctgcac gccaaggacg
    23941 gcgctacccg cgtgctgaaa gaaaccacca aggtcaaggc cggcgagctg atcgattcgt
    24001 ccgtgatgag caagaaggcc ctgcgcgcct tcatcaccga acagatcgcc gacgcccgcg
    24061 cccagggcgt gctgttctcg gtgcacctga aggccaccat gatgaaggtc tccgacccgg
    24121 tcatcttcgg ccacttcgtc tcggtgtact acgaggacgt gctggccaag cacgccgaca
    24181 cgctggcctc catcggcttc gaccccaaca acggcatcgg cgacctctac acccgcctcg
    24241 ccaccttgcc ctccgccaag caggccgaga tcaaggccga catcgcggcc gagtacgcca
    24301 agcgcccggc gctggccatg gtgaactccg accgcggcat caccaacctg cacgtgccca
    24361 gcgacgtcat cgtcgacgct tccatgcccg cgatgatccg cgagggcggc aagatgtgga
    24421 acgccgaggg caaggcgcag gacgccaagg ccatcatccc cgaccgctgc tactccggcg
    24481 tctaccaggc cgtcatcgag gactgcaagg tcaacggcgc cttcgacccc gtcaccatgg
    24541 gcaccgtgcc caacgtgggc ctgatggccc aggccgccga agaatacggt tcgcacgaca
    24601 agaccttcgt catccccgcc gacggcaccg tgaaggtgac cgacgcctcg ggcgccgtgc
    24661 tgctcgagca cgccgtggaa gccggcgaca tctggcgcat gtgccagacc aaggacgccc
    24721 ccgtccagga ctgggtcaag ctcgccgtca cccgcgcccg cctgtcgaac acgcccgccg
    24781 tgttctggct ggacccggcc cgcgcccacg acgcccaggt catcgccaag gtcgagcgct
    24841 acctgaagga ccacgacacc accggcctgg acattcgcat cctgtcgccc atcgaggcca
    24901 ccaagttctc gatcgaacgc atccgcaagg gcctggacac catctcggtg accggcaacg
    24961 tgctgcgcga ctacctgacc gacctgttcc ccatcatgga actgggcacc agcgccaaga
    25021 tgctgtcgat cgtgccgctg atggccggcg gcggcctgtt cgaaaccggc gcgggcggct
    25081 cggcccccaa gcacgtgcag cagttcctgg aagaagactt cctgcgctgg gattcgctgg
    25141 gcgagttcat ggccctggcc gcctcgctgg agcacctggg ccagtacgcc ggcaatgcca
    25201 aggccacgat cctggccaag acgctggacg aggccaccgg caagttcctc gacaccgaca
    25261 agtcgcccgc ccgcaaggtc ggcggcctgg acaaccgcgg cagccacttc tacctggccc
    25321 t
//
LOCUS       DASWPO010000040        40884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_989751, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000040 DASWPO010000000
VERSION     DASWPO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 40884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40884
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989751"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /gene="hslV"
                     /locus_tag="VGC69_02170"
     CDS             <1..463
                     /gene="hslV"
                     /locus_tag="VGC69_02170"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY0294122.1"
                     /translation="QVTLGNIVIKGTARKIRRLYHDKVLAGFAGATADAFTLQELFEA
                     KLEKHQGHLMRAAVELTRDWRTDRVLRRLEAMLIVADAEHTLVLTGTGDVLEPEHGLA
                     AIGSGGAYAQSAALALLRNTELPPETIVKQSLEIAGDICIYTNQNHVIETL"
     gene            591..1925
                     /gene="hslU"
                     /locus_tag="VGC69_02175"
     CDS             591..1925
                     /gene="hslU"
                     /locus_tag="VGC69_02175"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY0294123.1"
                     /translation="MSASTMTPGEIVSELDKYIIGQNKAKRAVAVALRNRWRRQQVEE
                     PLRHEIHPKNILMIGPTGVGKTEIARRLAKLANAPFIKIEATKFTEVGYVGRDVDTII
                     RDLTEFAVKQTRELEMRRVRSHAEDAAEDRILDVLVPPSRNAHGEPDRNEESPARQTF
                     RKRLREGQLDDTEIEIEIAQPIPQLDVMSPPGMEEMAEQLRGMFAGMARDKKKPKKMK
                     VKDAFKLIIEEEAGKRINEDDLKAAAVTNVEQNGIVFLDEIDKIAARQETGGADVSRQ
                     GVQRDLLPLVEGTTVNTRYGMVRTDHILFIASGAFHLSRPSDLIPELQGRFPIRVELE
                     SLTARDFVSILSDTDASLIKQYSALLATEDVLLEFAEDGIERLAELAFSVNERTENIG
                     ARRLYTVMEKLLEELSFDAGSHNGKTVKIDAAYVDAQLAETAGSQDLARYVL"
     gene            complement(1954..2955)
                     /locus_tag="VGC69_02180"
     CDS             complement(1954..2955)
                     /locus_tag="VGC69_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0294124.1"
                     /translation="MKYRRLGKSNLNISRLCLGTMMFGEQSPFEAAADIVASARDHGI
                     NFIDTADVYNGGKSEEVVGKLIAQDRDQWVLASKIGNPVGKGPNQSHYSRKWLIQGVE
                     QSLGRLGTDYMDILYLHRDFHEENLEELVLALGDLISSGKLRYFGISNFRGWRIAEIV
                     NACEKLGVPKPVVCQPYYNLLNRMPEVEILPACAHFGLGVVPYSPIARGVLTGKYAPG
                     QAPAADTRAGRGDKRIMDTEFREESLKIAGDLVAYAEGKGIKPGHFATAWVLANPIVT
                     AVIAGPRTLAQMEDYYGALEVSISAQEEAMVDEWVTPGHASTHGYNDPNYPFFGRPV"
     gene            complement(3057..4007)
                     /gene="speB"
                     /locus_tag="VGC69_02185"
     CDS             complement(3057..4007)
                     /gene="speB"
                     /locus_tag="VGC69_02185"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY0294125.1"
                     /translation="MQKILHQPQGGNDMPRFAGRGTMLRLPFVENLEGLDAAFVGVPL
                     DIGTSQRAGTRYGPRHIRNESVMIRPYNMATGAAPFDSMSVADIGDVPINTYNLLKSV
                     DIIEAYYARLNAHPLVPLTLGGDHTITLPILRALTRKHGPVGLIHVDAHTDTNDEMFG
                     EKIAHGTTFRRAVEEGLLDCQRVVQIGQRAQGYTAEDFRWGVKQGFRLVQAEQCWHKS
                     LAPLMAEVREQMGAGPVYLSFDIDGLDPIWAPGTGTPEVGGLTSIQGLEIVRGCRGLN
                     LIGCDLVEVSPPYDISGNTSQLAANLLYEMLCVLPGVQYR"
     gene            4209..5138
                     /locus_tag="VGC69_02190"
     CDS             4209..5138
                     /locus_tag="VGC69_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294126.1"
                     /translation="MKATGKHINLRLLEVFVAVVRHQGYARAQHELNLTVSAISNYMS
                     ELEDRLGYVLCQRGRSGFALTPKGEQFLQHSLRLLSEMEQFERQSDALKGDAGGTFRM
                     GVIDATANDPDLALPQLVGRFNEQFPAVHLNLMIRSPHDLLMGIQDNQLDLAVGSFPF
                     RVSNVIEVPLYREQHWLYCSDQHPLFAQGKLNLPLVTQYGIVTRSYWSSADLGEKGFK
                     RSTATVDNMEAQLILILSGKYVGYLPEHYAHSLLKEGRLRVLMPNEFGYQAEFSAIFR
                     RGRSKEPFIRAMREMLKSQIRKKSRGVRNALGS"
     gene            complement(5264..5539)
                     /locus_tag="VGC69_02195"
     CDS             complement(5264..5539)
                     /locus_tag="VGC69_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEY0294127.1"
                     /translation="MQPIPPEESLIKYPTDFPIKVMGKHHPDFAQTLTEIVQQFDPAF
                     DPATVEMRPSKGGNYLGLTFTVCATSREQLDSLYRALHGHPMVSIVL"
     gene            complement(5606..6472)
                     /locus_tag="VGC69_02200"
     CDS             complement(5606..6472)
                     /locus_tag="VGC69_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEY0294128.1"
                     /translation="MIAGMASDSQVYLNGEFMPVGEAKISVLDRGFIFGDGIYEVVPA
                     YNGKPFRLEHHLNRLDRSLAAIRIANPHDRAGWVALVGQLIERAPTPTCMVYLQVTRG
                     VAKRDHGFPKDPIAATVFGMVNAMTVPGKAQREQGLATISIPDERWLHCEIKSVSLLG
                     NVLAKQAAVDAGVDEVLQFRDGYLSEASSCNVWVASGGKLLAPPKDNLILEGIRYGLM
                     TELAAEAGVPFEARKLSKAEVQAADEIMLTSATKEVLPVVRLDGKPVGSGKPGPVYAK
                     LRERYDARIAAL"
     gene            complement(6570..7823)
                     /locus_tag="VGC69_02205"
     CDS             complement(6570..7823)
                     /locus_tag="VGC69_02205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY0294129.1"
                     /translation="MKKPLPSYARRAVAGLLVSAMVAASGVPAAYAAAAAPAAAAATP
                     AMPPFADPNSLVVGDISAVPAPTIAAKAWVVIDVTSGQTLAASNADMQVPPASLTKIM
                     TSYVAFTAVDEKRLTLDQQVPVSERAWRTGGSRMFIQPRLPVTVDELFQGLIVDSGND
                     SAVALAEAVAGSAESFVTLMNEQAAKLGMRNTHFMNVDGLPDPQHLTTARDLAIVASH
                     AITDHPQEFHYYKQREFVYNKIKQPNRNRLLWADSTIDGMKTGFTDAAGYCMIATALR
                     GDRRILTVVLGADSASSRAEESLKLLNWGFQNFDTVKLYGENQPGLDVRVWEGKAEMA
                     RLGPPQPVSLTVPRGKSGDIKPVAHYPDPLIAPLAKGQQVGTLELTLDGKTIQTEPLV
                     VQTEVERAGFFGRMMDMIKRRFSSK"
     gene            complement(7982..8635)
                     /locus_tag="VGC69_02210"
     CDS             complement(7982..8635)
                     /locus_tag="VGC69_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY0294130.1"
                     /translation="MAAHTEIVGFEGAAGRIDCALDWPDGTPRGWALVLHPHPLQGGA
                     RDNKVVTTIARACLHQGLAAVRPDFRGVGASEGAFDKSVGETQDMLALVAQFRERYPE
                     LAARPWVLGGFSFGTSVAAGLHAGLAAAGDARLPAALILAGAAVQRFQPADLPAPAHT
                     LMVHGERDEVVPCAETMDWARVRNLPVVVIPDASHFFHGKLLILRQLVLDRLTVALA"
     gene            complement(8677..9279)
                     /locus_tag="VGC69_02215"
     CDS             complement(8677..9279)
                     /locus_tag="VGC69_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY0294131.1"
                     /translation="MKRVTERRMKGIGALCLALALAGCSVGKVGPAPVLYDLGADDGA
                     AVSGGAARKPIALAFDAAPALGDTGMIWRVGGSASPKAYARAKWSVSPAELVRQRLVE
                     RLSRLGPVLSEGAAGLPRLQVTLTRFEQVFAADGTASTGQVSLQAVLLQADGRVLAQK
                     LVQRSAAAATQDAEGGARALRQATDGAADELSAWLGGALR"
     gene            complement(9276..10208)
                     /locus_tag="VGC69_02220"
     CDS             complement(9276..10208)
                     /locus_tag="VGC69_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0294132.1"
                     /translation="MENRSYALMAGLFTLALIAAAVLVALWLGHDKTAVVVYELVSSD
                     SVGGLTAQSAVRFQGVPVGRVQSLTLDTAQPGRVLIRIAVAPTTPVTDATWAELGVQG
                     ITGLAIVELHDQGKSEHRLATSSDHPAQIPLRPGLFSRIEEKGNVLLDDLDQAAGQFK
                     ALLSPENTQALSMMLKNAADISGQLKQMSGNLGPLSESLDGAVRKMGDAAQQAGAAMQ
                     DVRTAMARINAPDGPLATATLSLQQISQAAARLNGQTLPAMTDMAGKVGVTARSASSV
                     LRRVGEAPQSLLFGLPAVQPGPGEAGFAGFGEKR"
     gene            complement(10195..11001)
                     /locus_tag="VGC69_02225"
     CDS             complement(10195..11001)
                     /locus_tag="VGC69_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294133.1"
                     /translation="MGEAVQPMIEVRDLRTAFGDHVVHDKLNLSVLPGEILSIVGGSG
                     SGKTTLLRAIIGLDRPAAGTVHVLGHDLLELDYPEFRRLSRRWGMLFQAGALFSSLSV
                     FDNVAQPLRELRTLPEDLIGEVVMCRLAMAGLAENDADKQPSDLSGGMIKRVGLARAL
                     ALDPSLLFLDEPTAGLDPQRSDEFVALVRSLHQQLGFTVVMITHDLDTLVDLSSRVAV
                     LADKRVIACDTVEKVLQVDHPFIKEFFLGERGRRALGGLISKDMVGHGKP"
     gene            complement(11001..12125)
                     /locus_tag="VGC69_02230"
     CDS             complement(11001..12125)
                     /locus_tag="VGC69_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294134.1"
                     /translation="MTRVSTPSAQAVRQDGGVCYLAGDWTVGALAEAGMVASRRQALP
                     SAGQDLGWDLLGVERLDTVGAHVIWQAWGNRLPGQVRLSDRQRVVFQALADNTGGVHG
                     KPKHRADRLEWVRGVGDLWSGAAENGRSMLIMFGQLVLDLAGLLTRRWRVPWKPISAQ
                     VYHTGAQALGITALVGFLIGVVLSYLSAQQLRTFGADRFIVQLLGVSIVRELGPVLAA
                     ILVAGRSGSAITAEIGVMRVTQELDAMQVMGISHSRRLILPRVLALSVSMPLLVLWTD
                     AMALLGGALAAKAQLNISGMWFLQSLQGAISSTNYGIGLLKGATFGALIALISCHFGL
                     RIKANTESLGQGTTTSVVTSITGVILVDALYAIMFSSVGM"
     gene            12221..13069
                     /locus_tag="VGC69_02235"
     CDS             12221..13069
                     /locus_tag="VGC69_02235"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0294135.1"
                     /translation="MSHPSVPPLGLPAPQALARMLADRLPDFRQVGWTARTGSTNADL
                     LAQARERPEGKPWLLGAHLQTNGRGRVGRSWKNLPGAALMLSCAFDVHLPVATLPALS
                     PLAGLAACEGLRALIGGPGASRLAVKWPNDLQWGQAKLAGLLVESVRNPGGPGSGYTV
                     VIGIGLNLCDADALSQALERPIADWTQVDARHAIDAVDLACALAGSLHQAMLDAQRHG
                     FDTFAQHYARVDALAGQAVNVIDQGRILQQGIAQGIDAHGRLLLATPDGIQPIVVGEI
                     SIRPQS"
     gene            13066..13869
                     /locus_tag="VGC69_02240"
     CDS             13066..13869
                     /locus_tag="VGC69_02240"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY0294136.1"
                     /translation="MILLIDAGNSRLKAGLLPGDARRRLPDTTVFDNTNPQALADWLH
                     ALPAKPARAAGVNVAGTAHGDIIARLIRQACGCDIEWLPAQAQTLGLRNSYDHPGQLG
                     TDRWAAMLGILASLPAGHPPFMVASFGTATTLDTVSGDNVFLGGLILPGPAMMRAALA
                     RGTANLPMAQGVAASYPTNTSQAIATGVTAAQAGAVVRQWLAARTRFGTTPDIYVTGG
                     GWPEVRTEVETLLAQAGAARSVPTGPVYVESPVLDGLAAWALQPPRHPE"
     gene            13906..14052
                     /locus_tag="VGC69_02245"
     CDS             13906..14052
                     /locus_tag="VGC69_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294137.1"
                     /translation="MRILFLLIVLANIWAYALGQGWLGVRPGDENRGKLVQELNAEKV
                     ALGR"
     gene            complement(14059..14391)
                     /locus_tag="VGC69_02250"
     CDS             complement(14059..14391)
                     /locus_tag="VGC69_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294138.1"
                     /translation="MSGAMPVFDTYKYINALKAAGFTEAQAEAQSSALVGAFDAYGDR
                     LATKEDLRVLGAELRTEMAEIRGEMRGMRSEIDQVRNMLRWVAGPSIISMMGTVTGAV
                     LLLAKSMG"
     gene            complement(14488..15234)
                     /locus_tag="VGC69_02255"
     CDS             complement(14488..15234)
                     /locus_tag="VGC69_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294139.1"
                     /translation="MEKIVIVGATSAIAEHCARLWAGAGPVDLTLVGRDAGKLERVAA
                     DLRVRGGPSSQIRTAVADLSSTAAIEAMVADAVRAGPVDTVLIAQGSLPDQAHCQDDL
                     QACRQALEVNAVSPALCAEGFARHMAAAGRGTLALIGSVAGDRGRKSNYVYGSAKGLL
                     AGYAQGLQHRFAGKGVKVVLIKPGPTDTPMTAALKSEGARLAPVEEVAARIVKAIARG
                     QAVVYAPGKWWLIMMIIRHLPAVVFNRLDI"
     gene            complement(15257..16567)
                     /locus_tag="VGC69_02260"
     CDS             complement(15257..16567)
                     /locus_tag="VGC69_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0294140.1"
                     /translation="MRPVSAWGRLSQDAHDVVPLLDPVQAAGRIRASAWPGLAHGLGR
                     SYGDVGLNPGGTLWLASGLDRFLSFDEATGRLVCEAGVALRDIQRLAVPRGWMLPVTP
                     GTRWVTVGGAIANDVHGKNHHAQGSFGDHVLGIRLVRTDGQVIECGPRLQAPWFAATV
                     GGLGLTGVIVQAEIQLRRVQGPWLAAQTVPFAGLGEFFRLSDASEAGWEYTVAWIDCL
                     SASGRGLFMRANHGGAALQSGARPVPKAHKHAVPFVPPVSLVNRLSLRPFNAAYYHLK
                     KWRAGRNVVHYEPFFYPLDNLLEWNRLYGPKGFYQYQSVVPAEAGRDATQAMLQAIAR
                     SGEGSFLAVLKTFGKRRSPGMLGFPRPGVTLALDFPNRGARTHGLFERLDAIVREAGG
                     GIYPAKDARMPRDLFESAYPRLPEFAQYRDPGISSALSRRLMGY"
     gene            complement(16564..16977)
                     /locus_tag="VGC69_02265"
     CDS             complement(16564..16977)
                     /locus_tag="VGC69_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0294141.1"
                     /translation="MPDSSKLAATYVIFALIAMAANIGAQDVFVRVYDGLFAIAGAVV
                     VGTGVGLVVKYVLDKRYIFRFQAKSAVHDGQTFALYTLMGLATTAIFWGFEFGFQQAF
                     DSKSLRYLGGVIGLAIGYAVKYRLDKRYVFRTGPA"
     gene            17004..17888
                     /locus_tag="VGC69_02270"
     CDS             17004..17888
                     /locus_tag="VGC69_02270"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEY0294142.1"
                     /translation="MDDTAATCKPVTQPLGLLRLMRPKQWVKNGFVLAPLLFTGEFLD
                     AAAVRHALLALVLFCVASSATYIVNDLRDVEQDRRHPTKSRSRPLAAGIVTPPAALGL
                     LGVLYAILIGGWFAAPQVILVLISYLALNLAYTFVLKHQPVLDIFTIAIGFVLRVYAG
                     AMSLSVPVSSWMLITTLCLALYLAAIKRRQELQHSGASGRQILKKYSVALVDRYAEMS
                     ATGALLFYSMFVMSTRPELVLTIPLVLFGLFRYWFVVDSMDEGESPTDVLLRDWPLLL
                     TLVLWVAACGAALLAKQG"
     gene            17894..18310
                     /locus_tag="VGC69_02275"
     CDS             17894..18310
                     /locus_tag="VGC69_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEY0294143.1"
                     /translation="MDWSYLATPLLAWFVAGMLKFAINSLRARRLAFDLIGYGGLPSN
                     HSAIVSSMAALIGLKEGIAQPAFGVALTLALIVIMDAHSLRRQVGRHAEAINKMAQPA
                     SANHLPLRERMGHSRVEIAAGILVGIAAALAVRHFL"
     gene            complement(18314..19282)
                     /gene="waaC"
                     /locus_tag="VGC69_02280"
     CDS             complement(18314..19282)
                     /gene="waaC"
                     /locus_tag="VGC69_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEY0294144.1"
                     /translation="MSTRILIVRTSSLGDLIHTLPAISDIARYVPDARIDWVVEEAFA
                     EIPGWHPAVKEVIAVAHRRWRKTWWQAKTRQERRSVADRLGGVRYDVVLDMQSLLKSA
                     WLARQAQGTRHGLDWQSARESLASLFYDVRHRVAFLQPAVTRQRLLAGLTFGYDYAGA
                     PDFGLQGFAAAARNEQDRQGYAVIMPSASRDDKLWPEDDWRAVFERVRKAGCALRVLA
                     GNARETARARALTAGLEGVEVMPRMSLTAVANLLAGARLMVGLDSGLTHLSAALGRPT
                     IGIYRASTPVRTPLIGATYTASLGDQGAPPSRDAVLVAVEQALAAG"
     gene            19351..20454
                     /locus_tag="VGC69_02285"
     CDS             19351..20454
                     /locus_tag="VGC69_02285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0294145.1"
                     /translation="MRRIDQLAGPLLLRALALFRRRGNPPPGDLRAIGILCAVPLGDL
                     LISLSGMIPALRAKFPHARIIAFNAGFSRGAFALLPRVDKQITISLGNPLGAAALLRQ
                     EKLDVLIDLSQWANITAILSVLSGARHTIGFDTPHCARGRGYDTGVPHLDTRHELANY
                     AALVKPLGIAQISEPALRLPQAEVEQVRQRHADRYMVFHAWPSGINKHLKQWPAAHWR
                     ALAEKFIALGYAVVFTGAQGDRAATQALIDGLPASSKARAINAAGSLSLRETALLIQG
                     SAGLVSVNTGIMHIGAALDVPMAALHGPTNPLRWGPLSRKARVLIPSGPDVAYLNLGW
                     EYPPGAQETLPRLSVDEVFDSMLAMQRQSRQGD"
     gene            complement(20455..21906)
                     /gene="wbaP"
                     /locus_tag="VGC69_02290"
     CDS             complement(20455..21906)
                     /gene="wbaP"
                     /locus_tag="VGC69_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HEY0294146.1"
                     /translation="MLFKHPSSVRNNAKAILAQAILAVSDLLAFNLGLVIAILLVMAF
                     WGDLERFLPYQEMAPRFAAHGLLSLACMGWFWLRLRHYSYRKPFWFELKEVLSTVVIF
                     AVIDLAIIGFSKWQLSRYVWVFTWCGILVLLSVGRALTKKLLRRLKLWQKQCVIIGTG
                     SNAVDVFVALQSEAYLGFDIQYFVGCADQEEPAELLGLPVICDEQLLWHATDASNTQY
                     VIALEYSQEKVRENWLKQLAVHKCQSVSVVPSTRGLPLDSTDMMFIFRREVLILGINN
                     KLTKLSARTLKRGFDLISSFVLLLLCSPLLIGLAYLVMREGGRPIYGHLRVGQNGKLF
                     KCYKFRSMAVNADEILDRLLAADPAARAEWEQDFKLKDDPRITRIGRFLRRSSLDELP
                     QLFNVLKGEMSLIGPRPVVPEELERYGDNAEYYLMVKPGMTGLWQVSGRNDVSYETRV
                     YFDAWYVKNWSLWNDITILFKTIEIVFKRDGAY"
     gene            complement(21949..23103)
                     /locus_tag="VGC69_02295"
     CDS             complement(21949..23103)
                     /locus_tag="VGC69_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0294147.1"
                     /translation="MRVGFGTTGLAHGLARGGVDGIGSYTRELGGALLSTGDMALIPA
                     GFQAYATAETLPGAQMPADLGRFSTMALLGAFTPLACQAERALQDRIDLFHATDHLIP
                     KFSRIPVVATLMDAIPLSNPEWVRTRLISVKNWLWRRSGHWADHVITISEYSKQEIIR
                     YFGIDADKISVTPLGVHPRFFESFDAETKAEVLERLGLPERFFLFVGTLQPRKNLERL
                     LDAHARLPARLRADFPLVIVGRAGWKCEELVARVEALRASGEARWMQYLPDVEARVLM
                     QTAFALVFPSLSEGFGLPVVEAFASGLPVIASNTTSLPEVAGEAAILVNPEDADEMAQ
                     GMRQLIDNPGLVEQLRVAGMARAREFSWQACAEKTMAVYRTVLGASHQGR"
     gene            complement(23214..23909)
                     /locus_tag="VGC69_02300"
     CDS             complement(23214..23909)
                     /locus_tag="VGC69_02300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0294148.1"
                     /translation="MNKAEFDSFADEYRAMHAKNIAASGEGPDYFAEYKIKDIAQELI
                     RRPEKQSERILDFGAGVGNSIPWIKKYLADSELTCADVSARSLGVAAERFPGAAKFTS
                     FDGEHLPFPSGYFDLAYAMCVFHHIDHEMHVPLMEEISRTLSPGGVFVIFEHNPLNPL
                     TVRAVNTCEFDVNARLITAWKMKRACLLAGFRKVEVRYRIFFPRMFAGLRGLEKYMTR
                     LPLGAQYSVWAEK"
     gene            complement(23927..25993)
                     /locus_tag="VGC69_02305"
     CDS             complement(23927..25993)
                     /locus_tag="VGC69_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294149.1"
                     /translation="MPPSIQRLVADHWEKGLLLIFPTVLWWSYPLWIVDKTDLDVWFY
                     YGYFRHFFEFWNYFSIDEAHKYYGTRVPYIGLGHIFWHFLNPDAARYAFHALFLYPVI
                     ILCFWYVARVHTSRGVAFAAASMMGTDIFFIRDAGSDYVNTGVLLYQMLAFVCLARAV
                     KSVHRNLYLILAGFCAASMVFTHLVALVYLPLLVLYYLFFVTRQEPVRFIKLLGPTLS
                     YGIGGVIVAFVVWAGVFYYGTHDTTFFLKPQILMLFLDHASSWALSFGSLGTSSYWVT
                     IHWAALFGASMAVVWMCVNRKMPEPDIFFWIVIGPLFCGLLAIFKLDGLLLLYSRDGL
                     YATPLFALTYLTIAALLFHKKSLNMWAALGVFTVCVISLWIRLAIGSVELPVWAVAAL
                     IGVLCFLAFFFEEMARRWMALGLALLVGVATLLIPHEFDGRPIIYPIHEYIVEKAQGR
                     LPKIFWSEETRAEKRVGDTYFVSIESSFTERAHWQRGMDFPNCAQPDGGGLAAGDLVV
                     ILSTQPVGDDELARFQEKCVGDLRKAESRVFEDAKGKYYVQFLEVPLHGRLVAWNRDA
                     KELPGLVGHVDGADRVADDGKDKEGFLTYGPYAQILPGHYKVTLWYASDTDDNWWDST
                     ELRDNQIVVTSKGGLPNTHGQMRKLELDFVRAESVKNFELRTYFAGKGRMSVHRAAMV
                     SFPDPN"
     gene            complement(25993..26604)
                     /locus_tag="VGC69_02310"
     CDS             complement(25993..26604)
                     /locus_tag="VGC69_02310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0294150.1"
                     /translation="MSLYNRILGHPIIYNKIRPLIVGGIDLSNLYESLDVSAQDVVVD
                     VGCGTGDALNYLKSFKEYLGFDVDEVATSYASKRFASVANASFHARLFQTEDVEKIQP
                     TRVVLAGLLHHLTDEQAMDLLSMLAKSPRLRRIVSLDIVYLPGEWVSNAFAWCDRGRY
                     CRKQRGYEALAANAGLNVTKSIIMKSHPTHGRVKYLAMSMEPN"
     gene            complement(26662..27087)
                     /locus_tag="VGC69_02315"
     CDS             complement(26662..27087)
                     /locus_tag="VGC69_02315"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY0294151.1"
                     /translation="MDVIRRAKVFVSGKLDAVLIRQLIKFLLVGSVVAVVQFVLLFLF
                     VERIGIDATYASCIAYVICSMLNYFLNYRFTFQANISHGRALWRFWVIALIGLAMNGL
                     FMKIGTAVFMLNYIYAQICATGGVILWNFFANRKWTYAA"
     gene            complement(27087..28070)
                     /locus_tag="VGC69_02320"
     CDS             complement(27087..28070)
                     /locus_tag="VGC69_02320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0294152.1"
                     /translation="MQKISFVIPCYNEEEVIGETMQRLGALCDQLENLAAELIFVNDG
                     SQDNTLALLKSHAAHDPRVKIIGFARNFGHQLAATAGIDAASGDAVVLIDADLQDPPE
                     VVLQMVEKWREGYDVVYGTRTERPGESAFKLATARTFYRLLNKLSDVPIPLDTGDFRL
                     MSRPVVEILKAMPERDRFIRGMVSWAGFNQVALPYRRAERFAGESKYPLRKMLRFATD
                     GIMSFSCKPLQFSIGLGICSAVLALAGLIYVLAIRLFTSNWVEGWSALMVAVLFIGGV
                     QLISIGILGEYIGRIYGESKHRPLYVVGEYVGFAPSELESSRSPVVIIKSR"
     gene            complement(28226..29029)
                     /locus_tag="VGC69_02325"
     CDS             complement(28226..29029)
                     /locus_tag="VGC69_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294153.1"
                     /translation="MYHVDQLEISKDLCLVREQLKALMPDNPLVHGWASFSQCDEDGI
                     IRECLRRIAQVTACSRTFIEIGCADGLENNTHQLVLDGYKGVWVEGSQEKAAFMSEQL
                     GGLQFERLLIKNSFVDLGNISQLIQEAASFLHEKAVDFFSLDVDGNDWHLMKVAIDVI
                     SPKLICVEYNGKFPPPSHMVMEYNAAHEWMGDDYYGASLQAWIDALPGYTLVACNASG
                     ANAFFVRNDLAGGFTQYPVELLHQPPRYWLANGSLGHKNSLKWLRQAVQ"
     gene            complement(29086..30453)
                     /locus_tag="VGC69_02330"
     CDS             complement(29086..30453)
                     /locus_tag="VGC69_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294154.1"
                     /translation="MMTATDNDKAIELVDITKTYYTYAAPKQRLLEIISGGKKRYAKE
                     SKALDSVSFSLPKGGRLGIVGENGSGKSTLLKVLAGVLTPTSGSITVNGRVSALLELG
                     AGFNPLLPGKENIKQFCMLHGMSAEEVDEAMPDIIKFSELRDAVNQPVKTYSSGMAVR
                     LGFACAVYVKPEILIVDEALSVGDAYFQNKCLHKIKSLLDDGVTFIYVTHVADSIRTL
                     CDRGVWMDHGRVRLAGASSEVGAAYQSAIYRRVVHAGLAETEQDEQPKAIEADQDGGR
                     AYAIDHLRHKAFEERVSSLRTGSGEARILDIALIGENGQDTDTIAFNSKARIRVFYKI
                     EGPLPENCGLTLGVTDSSGKQVMHFNSIAQAGPLDANERNILQMMEFEFANPLCPGEY
                     GLIAGIGGLMKNPRNHGQMIANSIIDYAAGGARFSVQFPEAECEHDLWGLVYMPFSAS
                     NYKLD"
     gene            complement(30466..31272)
                     /locus_tag="VGC69_02335"
     CDS             complement(30466..31272)
                     /locus_tag="VGC69_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294155.1"
                     /translation="MNNVSFLEGLSLAWMLAKRDLKNRYAGSYAGMAWNIGVPLLYAL
                     VNVVVFSILVRGRMGEGYANVPFSLFYFVPFSLWIFFSETVNRSTGILREYSYLVNKI
                     SFPFWVLPLVPMASALLSQAVIIAITLMLMAYHGMALTTHAWGFLFVWLMCVIFTIGT
                     AYMVSALAVYIPDLSQAVPVAVNIMFWMTPILYPVALVQEHGGPVAKALLMDFNPFFY
                     WAEFSRSAIFGAESAITLLGAGILLLLSIIVAIIGALIFKKLKSGFADVL"
     gene            complement(31269..32402)
                     /locus_tag="VGC69_02340"
     CDS             complement(31269..32402)
                     /locus_tag="VGC69_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0294156.1"
                     /translation="MNVSFVSGVCVEHDAISNSIREEIQWLTKAGYHVRLFTYRCDYK
                     DLPYKQVGGLADVMYDPHYRHSDLIVWHFGVHYPLFNLLFIAPTRAKKVVVFHNITPK
                     QFVPESAHADIDLSFSQMSNIAVADQVVCDSDVNKEVLSAAGIDIPSITLPLAIRNNL
                     QAPVAKPSFSDNVSRIAFVGRFVRSKGVIELLEAIRRVMADDAQAKIQLDLVGNLDFS
                     DPVVLDALQSLMQRLRQDFHGRLAITLRGNAKESEKQAVLADADLFVLPTYHEGFCVP
                     ILEALASGCRVVAYDNSNVPFISGELADLVPTGDIGVLAKTMRDVLSQIGEPAWTNGS
                     KYRTFIDQAGRHVARFHPKTIEADFLRFVDSMVRPLDTRERLI"
     gene            complement(32399..34642)
                     /locus_tag="VGC69_02345"
     CDS             complement(32399..34642)
                     /locus_tag="VGC69_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0294157.1"
                     /translation="MAAHDTSPSFTIVINTLNRGPMLQKTLESFQWLKYSGQFEVVVV
                     NGPSTDNSDEIIASWLPRIRAGRCDVANLSVSRNVGICMARGDIVAFIDDDAVPEPEW
                     LEQMAAAYRDPQVGAVGGFVFNHTGYEYQYKYCLVDRFGNADLSPSRAFPHLSFPKSG
                     HFPHLLGCNSSFRRSALLKIGGFDEEYEYFLDETDVCLRIIDAGYLIEQLPNAFVHHK
                     YAPSNIRGDNKVVRYRYPVIKNKIYFMLKHAREFHPLDRIIQERDAFVQRQQDEVDRD
                     HGNGFISSADAERFSQDLVKAIEVGSKRGFEGVLPDAMITPSKRAKYAGTFLPFTPIQ
                     NPEFKTLVFVSRDFPPGHNGGIATFNKDLAEALAAKGNLVHVITQSPDINRVDFENGV
                     WVHRMASRDIPRSEAAVARNVPQSIWNWSATAFEEVERIGTHHPVDVVEAPIWDCEGI
                     AFLLARQWPLVTSLQTTLRFFLDHHPELESNPDWMRTHGTPMLALERELIGQSDLLRS
                     ISSAIRKDIEAAYGCSIPDERVCIAHLGMPDLAPSQRKPASDDAVEILFVGRLEPRKG
                     IDVLLEAIPEVLRQFPNATLRIVGDDTLPIPGQRKTYKETFLRSALDKTLIGRVHFDG
                     RVSDAQLLAAYEACDVFVAPSRFESFGLIFLEAMRAGKPVVGCAAGGMPELIEDGQSG
                     YLVPPGDAAALGQALMVLAGSKEKRRQMGERGRMRFLTSFTSEAMAQRSVELIARAMA
                     NFGNRCQ"
     gene            complement(34648..35544)
                     /locus_tag="VGC69_02350"
     CDS             complement(34648..35544)
                     /locus_tag="VGC69_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0294158.1"
                     /translation="MKSFVKKIIRRLARMPVIGPVVRMAIAVVRLPVNLERQRVFQNE
                     QLPLLLQAISDMNHRRLEHDNFIQSVPVSLRQNAQAIAEARESIAYLLGRVEFVRREL
                     MFEMRYGASTPAAEGEDQLKATTEVLNQEKLAQARAGRLCLNLGCGHVPLDGYLNVDR
                     RALPGVDIVSEINELPFGENELDEVFSAHLLEHFPQEQLRRELLPYFHKLLKEGGMFH
                     AVVPDAQAMMREFSNGNYPYDDMREVLYGGQDYDGDFHFNMFMPDSLTGLLQEAGFKD
                     VSVIEAGRRNGKSYEFEIVAKK"
     gene            complement(35555..36568)
                     /gene="galE"
                     /locus_tag="VGC69_02355"
     CDS             complement(35555..36568)
                     /gene="galE"
                     /locus_tag="VGC69_02355"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY0294159.1"
                     /translation="MAKAVLVTGGMGYIGSHTVVQLLGAGREIVILDNLSNSKLGVLD
                     RIERIAGRRPAFIQGDVRDPQAVRHVFDAHEIDSVVHFAGLKAVGEGEAKPLVYYDNN
                     VAGSLVLFQEMARAGIKTIVFSSSATVYGDPGYPNYREDTPLAPVNVYGRTKFLVEQI
                     LRDTCKADAGWRVAILRYFNPVGAHASGLIGEDPMGIPNNLMPFIAQVAVGKREFLSV
                     FGGDYPTPDGTGRRDYIHVEDLAAGHLAALTALAKSDAPITVNLGGGRSTSVLEIVAA
                     FGKVSGQSIPYKIVARRAGDLAEYYADPALAKQILGWEAQYGIERMCEDTWRWQTTNP
                     NGC"
     gene            complement(36649..37566)
                     /locus_tag="VGC69_02360"
     CDS             complement(36649..37566)
                     /locus_tag="VGC69_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0294160.1"
                     /translation="MKPAARSDAPRALVTGLGGFTGRYLAQELEAAGYRVFGTTYGDD
                     RTGPGMVNVDLCDREALAQAVAQIQPDIVAHLAAISFVAHGDVDAIYRTNVVGTYNLL
                     EALARQPRPPRAVLLASSANIYGNAAVEVIDESVAANPANDYAVSKLSMEYMARLWMN
                     RLPIVMTRPFNYTGVGQAAQFLLPKIVSHFQRGERVIELGNIDVERDFSDVRMVAAAY
                     MRLLDVAPAGGIYNVCSGKAVSLKAVIAAMEEIAGYQIEIRVNPAFVRANEVKRLQGD
                     AGNLIRAVGALRQIPLQETLRWMFDEGRW"
     gene            complement(37544..38578)
                     /gene="gmd"
                     /locus_tag="VGC69_02365"
     CDS             complement(37544..38578)
                     /gene="gmd"
                     /locus_tag="VGC69_02365"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0294161.1"
                     /translation="MKTSIVTGISGQDGAYLAQLLLEKGYTVYGTYRRTSSVNFWRIE
                     ELGIQNHPNLHLVEYDLTDLASSIRLLQTTGAAEVYNLAAQSFVGVSFDQPLTTAEIT
                     GLGAVNLLEAIRIVNPKARFYQASTSEMFGKVQAIPQTESTPFYPRSPYGVAKLYAHW
                     MTINYRESYDIFGCSGILFNHESPLRGREFVTRKITDSVAKIKLDKLDVLELGNMDAK
                     RDWGFAKEYVEGMWRMLQADAPDTYVLATNRTETVRDFVRMAFRATGVELDFQGKDES
                     EVGIDAATGRTLVKVNPKFYRPAEVDLLIGNPEKAKQKLGWEPKTKLEQLCSMMVEAD
                     LRRNETGCSF"
     gene            complement(38729..40156)
                     /locus_tag="VGC69_02370"
     CDS             complement(38729..40156)
                     /locus_tag="VGC69_02370"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY0294162.1"
                     /translation="MLIPVILSGGAGTRLWPVSREGYPKPFMKLADGESLLLKTYRRA
                     AAAAGKPAGEAGEILTVTNRDYYFMSKDELGLAKVGQSSTFLLEPEGRNTAPAVALAA
                     HCVAEKHGRDALMLVLSADHLIQDMGRFVQAVDAAVSLARQGYLATFGIVPSAPETGF
                     GYIEAGEALGEGKRVLRFVEKPSLDKAREYLQAGNFYWNSGMFCFKAGTILDELLRHA
                     PDVAQTVQACWETMTHDGAMLEIPPEAFSQVPDISIDYAVMERSDKVAVVPADFGWSD
                     IGSWVAVRELSKPDAENNRATGEAVFVDSHNTYVHGEGRLVAAVGVDNLMIIDTPDAL
                     LVVHPDKAQDVKQVVARLKKQNHEAYKLHRTVTRPWGTYTVLEEGPRFKIKRIEVKPG
                     ASLSLQMHHHRSEHWIVVSGMAKVVNGEREIFVSTNESTYIPAGHKHRLENPGVMDLV
                     MIEVQSGEYLGEDDIVRFQDIYGRA"
     gene            40351..>40884
                     /locus_tag="VGC69_02375"
     CDS             40351..>40884
                     /locus_tag="VGC69_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY0294163.1"
                     /translation="MRIADSQWIPSTPHQTWDALLDPQVLQQCLPGCIQATQLSPTEY
                     ALTLRTKIGGLECDYQGEILLSDVEPARSATLAFEGKGMAAGLAIGTAQVTLTEKEYG
                     TRLSYTVAGMAGGKLAELGEATVLKAGGKIVDKFFTAFVEYMARQPRTAAPPAPVEPE
                     ATGMSNSPWSWAVAAGVV"
BASE COUNT         8219 a        13455 c        11880 g         7330 t
ORIGIN      
        1 ccaggtcacc ctgggcaata tcgtcatcaa gggcacggcc cgcaagatcc gccgcctcta
       61 ccacgacaaa gtcctggcgg gctttgccgg cgccaccgca gacgccttca ccctgcagga
      121 gctcttcgaa gccaagctcg agaaacacca gggccacctg atgcgcgccg ccgtcgaact
      181 cacccgcgac tggcgcaccg accgcgtgct gcgacgcctg gaagccatgc tcatcgtggc
      241 cgacgccgaa cacaccctgg tgctcaccgg caccggcgac gtgctcgaac ccgagcacgg
      301 cctagccgcc atcggctcgg gcggcgccta cgcccagtcg gccgcgctgg ccctcttgcg
      361 caacaccgag ctgccgcccg aaaccatcgt caagcagtcg ctggaaatcg ccggcgacat
      421 ctgcatctac accaaccaga accacgtcat cgagacgttg taatcgtcct ctgcaatgcg
      481 gtgagccgcg gaccccggag gagcccgaag cgcaggcgcg agagcgccga gcatcggagt
      541 tccgcagggc agccgcggcg actcaagaac ccagatccgg aacagcaaaa atgtcggcct
      601 ccaccatgac ccccggagag atcgtctccg aactcgacaa atacatcatc ggccagaaca
      661 aggccaagcg cgccgtggcc gtggccctgc gcaaccgctg gcgccgccag caggtggaag
      721 agcccctgcg ccacgaaatc caccccaaga acatcctcat gatcggcccc accggcgtgg
      781 gcaagaccga gatcgcgcgc cgcctggcca agctggccaa cgcgcccttc atcaagatcg
      841 aggccaccaa gttcaccgaa gtgggctatg tgggccgcga cgtcgacacc atcatccgcg
      901 acctcaccga attcgccgtc aagcagaccc gcgaactcga aatgcgccgc gtgcgcagcc
      961 atgccgagga cgcggccgaa gaccgcatcc tcgacgtgct cgtgccgccg tcgcgcaacg
     1021 cccatggcga acccgaccgc aacgaggaaa gccccgcccg ccagaccttc cgcaagcgcc
     1081 tgcgcgaagg ccagctcgac gacaccgaaa tcgagatcga gatcgcccag cccatcccgc
     1141 agctcgacgt catgtcgccg cccggcatgg aagaaatggc cgagcagctg cgcggcatgt
     1201 tcgccggcat ggcccgcgac aagaaaaagc ccaagaaaat gaaggtgaag gacgccttca
     1261 agctcatcat cgaagaagaa gccggcaagc gcatcaacga ggacgacctc aaggccgccg
     1321 ccgtcaccaa tgtcgagcag aacggcatcg tcttcctcga cgagatcgac aagatcgccg
     1381 cccgccagga aaccggcggc gccgacgtct cgcgccaggg cgtgcagcgc gacctgctgc
     1441 cgctggtcga aggcaccacg gtcaacaccc gctacggcat ggtgcgcacc gaccacatcc
     1501 tgttcatcgc ctcgggcgcc ttccacctgt cgcgtccgtc cgacctcatc cccgagctgc
     1561 aggggcgctt tcccatccgc gtcgaactcg aatcgctcac ggcccgcgat ttcgtgagca
     1621 tcctgtccga caccgacgcc tcgctcatca agcagtactc ggcgctgctg gccaccgagg
     1681 acgtgctgct cgaatttgcc gaagacggca tcgagcgcct ggccgaactg gccttctcgg
     1741 tcaacgagcg caccgagaac atcggcgcgc gccgcctgta caccgtcatg gaaaaactgc
     1801 tcgaggagct gtccttcgac gccggctcgc acaacggcaa gacggtgaag atcgacgccg
     1861 cctacgtcga cgcccagttg gccgaaacgg ccggcagcca ggatctggcg cgctacgtgc
     1921 tgtgagggat agcggccgga atgagcagcc cgcttaaacc ggccgcccga agaacgggta
     1981 gttcgggtcg ttgtagccgt gcgtcgaggc atggcccggc gtcacccact catcgaccat
     2041 ggcctcttcc tgcgcgctga tcgacacctc cagcgccccg taatagtctt ccatctgcgc
     2101 cagcgtgcgc ggccccgcga tcacggcggt gacgatgggg ttggccagca cccaggccgt
     2161 ggcgaaatgc cccggcttga tgcccttgcc ctcggcatac gccaccaggt cgcccgcgat
     2221 cttcagcgat tcctcgcgga actccgtgtc catgatgcgc ttgtcgccgc gtccggcgcg
     2281 cgtatcggcc gccggcgcct ggcccggcgc atacttgccc gtgagcacgc cgcgcgcgat
     2341 cgggctgtag ggcaccacgc ccaggccgaa atgcgcgcag gccggcagga tttccacctc
     2401 cggcatgcga ttgagcaggt tgtagtaggg ctggcagacc acgggcttgg gcacgcccag
     2461 cttctcgcat gcattcacga tctcggcgat gcgccagccg cggaaattcg aaattccgaa
     2521 atagcgcagc ttgcccgaac tgatcaggtc gcccagcgcc agcaccagtt cctccaggtt
     2581 ttcctcgtgg aagtcgcggt gcaggtagag gatgtccatg taatcggtgc ccaggcgccc
     2641 gaggctctgc tccacgccct ggatgagcca cttgcgcgag taatgcgact ggttgggccc
     2701 cttgcccacc gggttgccga tcttgctggc cagcacccac tggtcgcggt cctgggcgat
     2761 gagcttgccc acgacctctt ccgacttccc gccgttatag acgtccgccg tgtcgatgaa
     2821 attgatgccg tggtcgcgcg ccgaggccac gatatcggcg gccgcctcga aaggcgactg
     2881 ctcgccgaac atcatggtgc ccaggcacag gcgggatatg ttgagattgc tcttgccgag
     2941 tcgacgatat ttcatgatgg cctgcgctcc tgaatgaatg aacaccggcc agtctacatc
     3001 cagaccccgg gcgcggccaa tgcgcttgcc cgcccgcgcg gcaatgcaag gctcgctcag
     3061 cgatactgca cgcccggcag cacgcagagc atttcgtaga gcagattggc cgccagctgc
     3121 gaggtgttgc cgctgatgtc gtacggcggc gaaacctcga ccaggtcgca gccaatcagg
     3181 ttcaggccgc ggcagccacg cacgatctcc aggccctgga tggacgtgag gccgcccact
     3241 tcgggcgtgc ccgtgcccgg cgcccagatc gggtccaggc cgtcgatgtc gaaggacagg
     3301 tacaccggac ccgcgcccat ctgctcgcgc acctccgcca tcagcggcgc cagcgacttg
     3361 tgccagcact gttcggcctg caccaggcga aaaccctgct tcacgcccca gcggaaatcc
     3421 tcggccgtat agccctgggc gcgctggccg atctgcacca cgcgctggca atcgagcagc
     3481 ccctcttcga ccgcgcggcg gaaagtggtg ccgtgcgcga tcttctcgcc gaacatctcg
     3541 tcgttggtgt cggtgtgcgc gtccacgtgg atcaggccca ccgggccgtg cttcctggtc
     3601 agggcgcgca ggatgggcag ggtgatggtg tggtcgccgc ccagggtcag cggcaccagc
     3661 ggatgggcat tcaagcgcgc gtagtacgcc tcgatgatat ccaccgactt gagcaggtta
     3721 taggtattga tgggcacgtc gccgatgtcg gccaccgaca tcgagtcgaa gggcgccgcc
     3781 cccgtggcca tgttgtacgg gcggatcatc accgactcgt tgcggatatg gcgcggcccg
     3841 tagcgcgtgc cggcgcgctg cgaggtgccg atgtccagcg gcacgccgac gaaggcggcg
     3901 tccaggccct ccaggttctc gacgaagggc agccgcagca tggtgccgcg gccggcaaag
     3961 cgcggcatgt cgttgccgcc ctgcggttga tgaagaattt tttgcatgat gtggaactcc
     4021 agcgtgccag cctttgagcg gggctgggcg gaataagcag gtcttatagt gcgacgccgc
     4081 cgcgaggcat aaaatacggt tttcgcaaaa aatcagttca gcattccctg aactatcccc
     4141 gctgcccggg ggattttcca cccacaccgc cactacccta gggaaacccc caggcctcgg
     4201 tcagccccat gaaagccacc ggcaagcata tcaacctgcg gctcctcgaa gtcttcgtgg
     4261 ccgtggtgcg ccaccagggc tacgcccgcg cccagcacga gctcaatctg acggtctcgg
     4321 ccatcagcaa ctacatgagc gagctggaag accgcctggg ctacgtgctg tgccagcgcg
     4381 gccgctcggg cttcgcgctc acgcccaagg gcgagcagtt cctgcagcac agcctgcgcc
     4441 tgctgtccga gatggagcag ttcgagcgcc agtcggacgc cctgaagggc gatgcgggcg
     4501 gcaccttccg catgggcgtg atcgacgcca ccgccaacga cccggacctg gccctgcccc
     4561 agctcgtcgg ccgcttcaac gagcagttcc cggccgtgca cctgaacctg atgatccgca
     4621 gcccgcacga cctgctcatg ggcatccagg acaaccagct cgacctggcc gtcggctcct
     4681 ttcccttccg ggtgagcaat gtgatcgagg tgccgctcta tcgcgagcag cactggctgt
     4741 attgcagcga ccagcatccg cttttcgcgc agggcaagct caacctgccg ctggtcacgc
     4801 aatatggcat cgtcacccgc agctactgga gctcggccga cctgggcgag aaaggcttca
     4861 aacgcagcac cgccaccgtc gacaacatgg aggcccagct gatattgatc ctgtcgggca
     4921 aatacgtggg ctacctgccc gagcactatg cccattccct gctcaaggaa ggccggctgc
     4981 gcgtgctcat gcccaatgag ttcggctacc aggccgagtt ctcggcgatt tttcgccgcg
     5041 gacgcagcaa ggagcctttc atccgtgcga tgcgggagat gttgaagtcg cagatccgca
     5101 agaaatcgcg tggcgtgcgc aatgctttgg gttcttaagg cgccgaggct gccctgcgga
     5161 attccgatgc tcgcccccag tgggggctgc gcttcggatt cctccggggt cctcggcttg
     5221 cgtcagccta atgcggctgt tcgtatcgct tgcgtgcggg gcatcacaag acgatggaca
     5281 ccatcgggtg cccgtgcagg gcgcggtaga gcgaatcgag ctgctcgcgc gaagtggcgc
     5341 agacggtgaa ggtcaggccc aggtaattgc cgcccttgct cggccgcatc tcgaccgtgg
     5401 ccggatcgaa ggccgggtcg aactgctgca cgatttcggt gagcgtctgc gcaaagtccg
     5461 gatgatgctt tcccatcacc ttgatgggaa agtcggtcgg atacttgatc agcgattctt
     5521 ctggcggaat gggttgcatg gctattcccc gtagcgcgga cggtcccgcc gggcaacggc
     5581 gggcacgccc atcatcgact cataatcaga gcgccgcaat acgcgcatca taccgctcgc
     5641 gcaatttggc atacaccggg cccggcttgc ccgaacccac cggcttgccg tcgagccgga
     5701 ccacgggcag cacctccttg gtggccgagg tcagcatgat ctcgtcggcg gcctgcacct
     5761 cggccttgga cagcttgcgc gcctcgaagg gcacgcccgc ttccgcggcc agctcggtca
     5821 tcagaccgta gcggatgccc tcgaggatga ggttgtcctt gggcggggcc agcagcttgc
     5881 cgcccgaggc cacccacaca ttgcacgaag aggcctcgct caggtagccg tcgcggaact
     5941 gcagcacctc gtcgacgccg gcgtcgaccg cggcctgctt ggccagcacg ttgcccagca
     6001 gcgacaccga cttgatctcg cagtgcagcc agcgctcgtc cgggatgctg atggtggcca
     6061 gcccctgttc acgctgcgcc ttgcccggca ccgtcatggc gttgaccatg ccgaacaccg
     6121 tggccgcgat gggatccttg ggaaagccgt ggtcgcgctt ggccacgccg cgcgtcacct
     6181 gcaggtagac catgcaggtc ggcgtcggcg cgcgctcgat gagctggccc accagcgcca
     6241 cccacccggc acggtcgtgc gggttggcga tgcggatggc cgcgaggctg cggtccaggc
     6301 gattgaggtg atgctcgagc cggaagggct tgccgttata ggcgggcacc acttcgtaga
     6361 taccgtcgcc gaaaatgaag ccgcggtcga gcaccgaaat cttggcctcg ccgaccggca
     6421 tgaattcgcc gttgagataa acctggctgt cgctcgccat gcccgcaatc atggactgct
     6481 cctgaaaaag aaaggctcgc cggtgggcgg cgagcacatt gctgcattat cgaaaaccgg
     6541 cgccgctgcg gcgccggcca tgcgttggat cacttgctgg agaaacggcg tttgatcatg
     6601 tccatcatgc ggccgaagaa gccggcgcgc tcgacttcgg tctgcaccac cagcggttcg
     6661 gtctggatgg tcttgccgtc cagggtcagc tcgagcgtgc ccacctgctg gcccttggcc
     6721 agcggcgcga tcagcgggtc gggataatgc gccaccggct tgatgtcgcc gctcttgccg
     6781 cgcgggacgg tcagcgacac cggctggggc gggcccaggc gcgccatttc ggccttgcct
     6841 tcccagacgc gcacgtccag acccggctgg ttctcgccgt agagcttgac ggtgtcgaag
     6901 ttctggaagc cccagttcag cagcttgagg ctttcctcgg cgcgcgaaga agcgctgtcg
     6961 gcacccagca ccaccgtcag gatacggcga tcgccacgca aggcggttgc gatcatgcaa
     7021 tagccagccg cgtcggtgaa gccggtcttc atgccgtcga tggtggagtc ggcccacagc
     7081 aggcggttgc ggttgggctg cttgatcttg ttgtagacga actcgcgctg cttgtagtag
     7141 tggaattcct gcgggtggtc ggtgatggcg tgcgaggcca cgatggccag gtcgcgcgcg
     7201 gtggtgagat gctgcggatc gggcaggccg tcgacgttca tgaaatgcgt gttgcgcatg
     7261 cccagcttcg cggcctgctc gttcatcagc gtgacgaagg attcggcgct gcccgccacg
     7321 gcctcggcca gcgccaccgc ggagtcgttg cccgaatcca cgatgaggcc ctggaagagc
     7381 tcatcgacgg tgaccggcag gcgcggctgg atgaacatgc gcgagccacc cgtgcgccag
     7441 gcgcgctcgg acaccggcac ctgctggtcg agcgtgaggc gcttttcgtc gaccgcggtg
     7501 aacgccacgt aggacgtcat gatcttggtg agcgaggcgg gcggaacctg catgtcggcg
     7561 ttggacgccg ccagcgtctg gccgctggtg acgtcgatca cgacccaagc cttggccgcg
     7621 atcgtgggcg cgggcacggc ggagatatcc cccaccacca gcgagttggg atcggcaaac
     7681 ggcggcatcg ccggggtagc ggcggcagcg gccggcgcgg cggccgccgc ataggccgcg
     7741 ggcacgcccg aggcggcgac catggccgac accaacaagc cggccacggc acggcgggca
     7801 taagacggga ggggcttctt catcagcaac aacatccagg acagaggcaa acaataactt
     7861 gccattatag gcagcgtccc agggacgtcg gcggcggcca tgcccccctt tttgcaagaa
     7921 gtgcaacgca atctatgcac gccatgacaa acacctgacc ggcgcgccgg ggccggccgc
     7981 cctaggccag ggcgacggtc aaccggtcga gtaccagctg ccggagaatc aacagcttgc
     8041 catggaaaaa atgcgaggcg tcgggaatca ccacgacggg caggttgcgc acccgggccc
     8101 agtccatggt ctcggcgcaa ggcaccacct cgtcccgttc gccatgcacc atcagggtat
     8161 gcgccggcgc cgggaggtcc gccggctgga agcgctgcac ggcggcaccg gccaggatca
     8221 gcgcagccgg cagccgcgca tcgcccgcgg ccgccaggcc ggcatgcagg ccggccgcca
     8281 ccgacgtgcc gaaagaaaac ccgcccagca cccagggccg cgccgcaagt tcgggatagc
     8341 gctcgcggaa ctgcgccacc agcgccagca tgtcctgggt ttcgcccacc gacttgtcga
     8401 acgcgccttc cgatgcgccc acgccgcgga aatccggccg caccgccgcc agcccctgat
     8461 gcaggcaggc ccgcgcaatg gtcgtcacca ccttgttgtc gcgcgcgccg ccctgcaggg
     8521 gatgcggatg cagcaccagg gcccagccgc gcggcgtgcc atcgggccag tcgagcgcgc
     8581 aatcgatgcg gcccgccgcc ccttcaaaac ccacgatttc agtatgtgcc gccatatcaa
     8641 aacctgattc gtttgaacaa caaaacccta ccgggctcag cgcagcgcgc cgcccagcca
     8701 agccgacaat tcatcggcgg caccgtcggt cgcctgccgc aaggcgcgcg cgccgccctc
     8761 ggcatcctgc gtcgcggcgg cggcggaacg ctgcaccagt ttctgcgcca gcacgcggcc
     8821 gtcggcctgc agcagcacgg cctgcaagga cacctggccg gtgctcgccg tgccatcggc
     8881 cgcgaacacc tgttcgaacc gcgtcagcgt cacctgcagg cgcggcaggc cggccgcccc
     8941 ctcggacagc accgggccca ggcgcgacag gcgctcgacc aggcgctgcc tgaccagctc
     9001 cgccggcgac accgaccact tggcccgggc ataggccttg ggactggcgc tgccgcccac
     9061 gcgccagatc atgccggtat cgcccaaggc aggcgcggcg tcgaaggcca gggcgatcgg
     9121 cttgcgcgcc gcgccgcccg aaaccgccgc gccgtcatcg gcgcccaggt cgtacaggac
     9181 cggggccggc cccaccttgc ccacgctgca gccggccaag gccagcgcca ggcacaaggc
     9241 gccgatcccc ttcatgcggc gttccgtcac gcgcttcatc gtttttctcc aaacccggca
     9301 aacccggcct cgcccggccc cggctgcacc gccggcaagc cgaacaacag cgattgcggt
     9361 gcttcgccca cgcggcgcag gacggacgac gcgctgcgcg ccgtcacgcc caccttgccg
     9421 gccatatcgg tcatggccgg cagcgtctgc ccattcagcc gcgccgctgc ctggctgatc
     9481 tgctgcaggc tcagcgtcgc cgtggccaag gggccgtccg gcgcattgat ccgggccatg
     9541 gcggtccgga catcctgcat cgcggcgccg gcttgctgcg cggcatcgcc catcttcctg
     9601 accgcgccgt cgaggctctc ggacagcggc cccaggttgc cgctcatctg cttgagctgc
     9661 cccgaaatat cggccgcatt cttcagcatc atcgacagcg cctgcgtgtt ttccggcgag
     9721 agcagcgcct tgaactgccc ggcggcctgg tccaggtcgt ccagcagcac attgcccttt
     9781 tcctcgatgc gcgagaacaa ccccggccgc aagggaatct gcgccggatg gtccgacgac
     9841 gtcgccagcc tgtgctccga cttgccctgg tcgtgcagtt cgacgatggc caggccagtg
     9901 atgccctgca cgcccagctc cgcccaggtc gcatcggtca ccggcgtggt gggcgccacg
     9961 gcgatgcgga tgagcacgcg gccaggctgg gccgtgtcca gcgtcagcga ctgcacgcgc
    10021 cccacgggca ccccctggaa gcgcacggcc gactgggccg tcaagccgcc caccgagtcc
    10081 gacgacacga gctcgtagac caccacggcc gtcttgtcgt gccccagcca gagcgccacc
    10141 agcactgccg cggcgatcag ggccagggtg aaaagccccg ccatcaaggc ataactacgg
    10201 ttttccatgc cccaccatat ccttggatat caacccgccc agcgcgcgac ggccgcgctc
    10261 gcccaggaaa aactctttga tgaaaggatg atccacctgc agcaccttct ccaccgtatc
    10321 gcacgcaatc acgcgcttgt cggccagcac cgccacgcgg gaagacaggt cgaccagggt
    10381 gtcgagatcg tgcgtgatca tcaccaccgt aaaacccagc tgctgatgca ggctgcgcac
    10441 caacgccacg aactcgtccg agcgctgcgg gtccaggccg gccgtgggct cgtccagaaa
    10501 cagcaggctc gggtccaggg ccagcgcgcg ggccaggccc acgcgcttga tcatcccgcc
    10561 cgacaaatcc gaaggctgct tgtcggcgtc attttcagcc agccccgcca tggccaggcg
    10621 gcacatcacc acctcgccga tcaagtcttc cggcagcgtg cgcaactcgc gcaggggctg
    10681 ggccacattg tcgaacacgg acaaggacga aaacagcgcc cccgcctgga acagcatgcc
    10741 ccagcgccgc gacagccgcc ggaattccgg ataatccagc tccagcaaat catgtcccag
    10801 cacgtgcacg gtgccggccg cgggccgatc gagcccgatg atggcgcgca atagcgtggt
    10861 cttgcccgaa cccgacccgc cgacgatgga caggatctcg cccggcagca cggacaggtt
    10921 gagcttgtcg tggacgacat ggtcgccgaa ggccgtgcgc aagtcgcgca cctcgatcat
    10981 cggctgcacg gcttcgccca tcacattccc accgaactga acatgatcgc gtacagcgcg
    11041 tccaccagaa tcaccccggt gatcgacgtc accaccgacg tcgtcgtgcc ctgccccagg
    11101 ctctccgtat tggccttgat gcgcaggccg aaatggcagg agatcagcgc gatcagcgcg
    11161 ccgaacgtgg cgcccttgag caagcctatg ccatagttcg tcgacgagat cgccccctgc
    11221 agcgactgca gaaaccacat gcccgagatg ttcagctgcg ccttggccgc cagcgcgccg
    11281 cccagcaacg ccatggcgtc cgtccagagc accagcaagg gcatggaaac gctcagcgcc
    11341 agcacccgcg gcaggatcag ccggcggctg tgcgagatac ccatcacctg catggcgtcg
    11401 agctcctgcg tcacgcgcat caccccgatc tcggccgtga tggccgagcc ggagcgcccc
    11461 gccaccagga tcgccgccag caccggcccc agttcgcgca cgatggacac acccagcaat
    11521 tgcacgatga agcgatccgc gccgaaggtg cgcagctgct gcgccgacag atacgacagc
    11581 accacgccga tcaggaaccc caccaacgcc gtaatgccca gcgcctgcgc gcccgtgtga
    11641 tacacctgcg ccgagatcgg cttccagggc acgcgccagc gccgcgtcaa aagccccgcc
    11701 aggtccagca ccaattggcc gaacatgatg agcatcgacc ggccgttctc ggccgcgccg
    11761 ctccacaaat cgcccacccc gcgcacccac tccaggcggt cggctcggtg cttcggcttg
    11821 ccatgcacgc cgcccgtgtt gtcggccagc gcctggaaca ccacgcgctg cctgtccgac
    11881 aagcgcacct gcccgggcag gcggttgccc catgcctgcc agatcacatg cgcgcccacc
    11941 gtgtcgagcc gctccacgcc cagcaaatcc cagcccaggt cctgccccgc ggacggcagg
    12001 gcctgccgcc ggctggccac catcccggcc tcggccaggg cgcctaccgt ccagtcgccc
    12061 gccagatagc acacgccgcc gtcctgacgg acggcctgag cggagggagt cgaaacgcgg
    12121 gtcatgggga tacggaacag ccgggccggt gctgaggaat gagacgcgcc catgatatac
    12181 gggaccagga aaatcagcgc tataacctac aattgcgacc atgtcgcacc catctgtccc
    12241 acccctcggc ctgccggccc cgcaagcgct cgcgcgcatg ctggccgatc ggttgccgga
    12301 cttccggcag gtcggctgga cagcccggac cggctccacc aacgccgacc tgctggccca
    12361 ggcgcgcgaa cgcccggaag gcaagccctg gctgctgggc gcccatctgc aaaccaacgg
    12421 ccgcggccga gtgggccgat cgtggaagaa cctgcccggc gcggcgctga tgctgtcctg
    12481 cgccttcgac gtgcacctgc ccgtcgccac cctgccggcc ctctccccgc tggccggcct
    12541 ggcggcctgc gaaggcctgc gcgccctgat cggcggcccc ggggccagcc ggctggccgt
    12601 caaatggccc aacgacctgc agtggggcca ggccaagctg gcgggcctgc tggtggaatc
    12661 cgtacgcaac ccgggcggcc ccggcagcgg atacaccgtc gtcatcggca tcggcctgaa
    12721 cctgtgcgat gccgacgcgc tctcccaggc cctggagcgc cccatcgctg actggaccca
    12781 ggtcgatgcg cgccacgcca tcgacgccgt cgacctcgcc tgcgccctgg ccggcagcct
    12841 gcaccaggcc atgctcgacg cgcagcgtca tggcttcgac accttcgccc agcactatgc
    12901 ccgggtggac gcactggcag gccaggccgt caacgtcatc gaccagggcc gcatcctgca
    12961 gcaaggcatc gcccagggca tcgacgcgca cggccgcctg ctgctggcca cgcccgacgg
    13021 catccagccc atcgtcgtgg gcgaaatctc catacggcca caatcatgat cctgctcatc
    13081 gatgccggca acagccgact caaggccggc ctgctgccag gcgacgcgcg gcgccgcctg
    13141 cccgacacca cggtcttcga caacaccaac ccgcaggccc tggccgactg gctgcatgcc
    13201 ctgcccgcca agcccgcgcg cgccgccggc gtcaacgtgg ccggcaccgc gcacggcgac
    13261 atcattgccc gcctcatcag gcaagcctgc ggctgcgaca tcgaatggct gcccgcccag
    13321 gcgcaaaccc tgggcctgcg caacagctac gaccaccccg ggcagctggg caccgaccgc
    13381 tgggccgcca tgctcggcat actggccagc ctgcccgccg gccacccccc cttcatggtg
    13441 gccagcttcg gcacggccac cacgctggac accgtcagcg gcgacaacgt cttcctcggc
    13501 gggctgatcc tgcccggccc ggccatgatg cgcgccgccc tggcccgagg caccgccaac
    13561 ctgccgatgg cgcagggcgt ggccgcctcc taccccacca acaccagcca ggccatcgcc
    13621 acgggcgtca cggccgcgca ggccggcgcc gtcgtgcgcc aatggctggc cgcgcgcacg
    13681 cggttcggca ccacgccgga catttatgtg acgggcggcg gatggcccga agtgcgaacc
    13741 gaagtggaga ccctgctggc ccaggccggc gccgcccgca gcgtgcccac cggaccggtt
    13801 tacgtggaaa gcccggtgct ggacggactg gccgcctggg cattgcaacc gccgcggcac
    13861 ccggaataat ccgggcaagc gcgcgcatta cggacggacc gcgacatgcg cattttgttc
    13921 ttgctgattg tgctggccaa tatctgggcc tacgccctgg gacagggctg gctgggcgtg
    13981 cgcccgggag atgaaaaccg cggcaagctg gtgcaggaat tgaatgcgga gaaggtggcg
    14041 ttggggcgtt gagccggatc agcccatgga tttggccagc agcagcacgg ccccggtcac
    14101 cgtgcccatc atgctgatga tggacgggcc ggcgacccag cgcagcatat tgcggacctg
    14161 atcgatctcg ctgcgcatgc cacgcatctc gccgcggatc tcggccatct cggttcgcag
    14221 ctccgccccc agcacgcgca agtcttcctt cgtggccagg cggtcgccgt acgcatcaaa
    14281 cgcgcccacg agcgcggacg actgcgcctc ggcctgggct tcggtgaagc ccgcggcctt
    14341 gagggcgttg atgtacttgt aggtatcgaa gacaggcatg gcgccggaca tgggaagcct
    14401 cggtatcggt catattagag gcctgcaata tgaccgcgca agccccggca gtccattcgt
    14461 ggataccgaa atggatcaaa ccgtccctca gatatcgagt ctgttgaaca cgaccgccgg
    14521 caagtgcctg atgatcatca tgatcaacca ccacttgcct ggcgcataaa ccaccgcctg
    14581 cccgcgcgcg atcgctttga cgatccgcgc agccacctcc tccacgggcg ccagcctcgc
    14641 cccctcgctt ttcaaggccg ccgtcatcgg cgtatccgtg ggcccgggct tgatcagcac
    14701 cactttcacg cccttgcccg cgaagcggtg ctgcaagcct tgggcatatc ccgccagcag
    14761 tcccttggcc gatccgtaga cataattgga cttgcgcccc cggtcgcccg ccacggagcc
    14821 tatcaaggca agcgtgcccc ggcccgccgc ggccatatgg cgggcaaagc cctccgcgca
    14881 caatgccggc gagaccgcat tgacttcaag cgcctgccga cacgcttgca gatcgtcctg
    14941 gcaatgcgcc tggtcgggca aggatccctg cgcgatcagc accgtgtcca ccggcccggc
    15001 ccgcaccgca tcggccacca tggcctcgat ggcggccgtg cttgataaat cggccacggc
    15061 cgtgcggatt tgcgatgacg gaccgccccg cacccgcagg tccgccgcca cgcgctcgag
    15121 cttgcctgca tcgcgcccga ccagcgtcaa atccaccggc cccgcccccg cccaaagcct
    15181 ggcgcaatgt tccgcgatgg ccgaggtcgc gccgacgatg acgatttttt ccatattctt
    15241 ctcgataccc cgtttctcaa taccccatta accggcgcga caaggcggaa ctgatgcccg
    15301 ggtcccggta ctgcgcaaat tccggcaggc gcggataggc cgattcgaac aagtcacgcg
    15361 gcatgcgcgc atccttggcg ggataaatgc cgccccccgc ctcgcgcaca atggcatcca
    15421 gccgctcgaa cagcccgtgc gtgcgcgccc cccgattggg gaaatccagc gcaagcgtca
    15481 cgcccggacg gggaaagccc agcatgcctg gcgagcgacg cttgccgaag gttttcaaca
    15541 ccgccaggaa agagccctcg cccgaccggg caatggcttg cagcatggct tgcgtggcgt
    15601 cacggcccgc ctcggccggc acgacgctct gatattgata aaagcccttg ggaccataca
    15661 ggcggttcca ttcgagcagg ttgtccaggg gatagaaaaa cggttcgtaa tgcaccacgt
    15721 tccgtcctgc ccgccatttt ttcagatggt aataggccgc gttgaagggc cgcagcgaca
    15781 ggcggttcac cagcgatacc ggcggcacga acggcaccgc gtgcttgtgc gccttgggca
    15841 caggccgggc gcccgactgc agcgccgcgc cgccatggtt ggcgcgcatg aacaggcccc
    15901 gtcccgatgc cgacaggcaa tcgatccacg caaccgtgta ctcccacccg gcctccgatg
    15961 catcggacaa gcggaaaaac tcacccaggc ccgcgaaggg cacggtttgc gccgccagcc
    16021 acggcccctg cacccggcgc aactggatct cggcctgcac gatgacgccc gtcagcccca
    16081 ggccgccgac cgtggcggcg aaccatggcg cctgcaggcg cggaccgcat tcgatgacct
    16141 ggccgtccgt gcggaccagg cggatgccca gcacatggtc gccgaacgac ccctgcgcat
    16201 gatggttttt gccgtgcaca tcattggcga tggccccgcc caccgtcacc catcgagtgc
    16261 ccggcgtcac cggcagcatc cagccgcgcg gcacggccag ccgctggatg tcgcgcagcg
    16321 ccacccccgc ctcgcagacg aggcggcccg tggcttcgtc aaaagacaga aagcgatcca
    16381 ggcccgaggc gagccatagc gtgccgcccg gattcaggcc cacgtctcca tagctgcggc
    16441 ccaggccatg ggccaggccg ggccaggccg aggcccggat ccggccggcc gcctggacgg
    16501 gatcgagcag cggcacgacg tcgtgcgcat cctggctcaa ccgcccccag gcggataccg
    16561 gcctcatgca ggccccgtgc gaaatacgta gcgcttgtcc agccggtact tgaccgcata
    16621 gccgatggcc aggccgatga cgccgcccag gtagcgcagg gacttgctgt cgaaagcctg
    16681 ctggaagccg aactcgaagc cccagaaaat ggccgtggtg gcgaggccca tcaaggtata
    16741 cagggcgaag gtctggccat cgtgcaccgc gctttttgcc tgaaagcgga agatataccg
    16801 cttgtccagc acgtacttga ccaccaggcc caccccggtg cccacgacca ccgcgccggc
    16861 aatggcgaac aggccatcgt agacgcgcac gaaaacatcc tgcgcgccga tgtttgccgc
    16921 catggcgatc aatgcgaaaa tgacgtaagt tgccgccaac ttgcttgaat caggcatcca
    16981 atacacccaa atagccaaag cccatggatg ataccgcagc aacctgcaag cccgtcacgc
    17041 agccgctggg cttgctcagg ctcatgcggc ccaagcaatg ggtaaagaac ggattcgtgc
    17101 tggcgcccct gctgtttacc ggggagtttc tcgacgcggc cgccgtgcgc catgccttgc
    17161 tggcgcttgt cctgttctgc gtcgcctcgt cggccacgta catcgtcaac gacctgcgcg
    17221 acgtcgagca ggataggcgg catcccacaa aatcccgcag ccgaccgctg gccgccggca
    17281 tcgtgacccc gcccgcggcc ctgggcctgc tgggcgtgct gtatgccatc ctcatagggg
    17341 ggtggttcgc cgcgccgcag gtcatcctgg tgctcatcag ctatctggcg ctcaacctgg
    17401 cctatacctt cgtgctcaag caccagcccg tcctggatat tttcacgatc gccatcggct
    17461 tcgtgctgcg ggtctatgcc ggcgccatgt cgctgagcgt tccggtttct tcgtggatgc
    17521 tcatcaccac cttatgcctg gccctgtacc ttgccgccat caagcggcgg caggagctgc
    17581 agcatagcgg ggccagcggc cggcaaatac tgaaaaagta ttcggtcgcc ctggtggacc
    17641 gctatgcgga aatgtccgcc accggcgcct tgctgttcta cagcatgttc gtcatgtcca
    17701 cgcggccgga gctggtgctg accattcccc tggtgctgtt cggcctgttt cgctattggt
    17761 tcgtcgtcga cagcatggat gaaggagaat cgcccacgga tgtgctgctg cgcgattggc
    17821 ctctcctgct gacccttgtt ctatgggtcg ccgcatgcgg ggccgccttg ctggcgaagc
    17881 aggggtaagg gcaatggact ggtcctacct cgccaccccg ctgcttgcct ggttcgtcgc
    17941 cggaatgctg aagttcgcca tcaacagcct gagagcccgg cgccttgcct tcgacctgat
    18001 cggctatggc ggcctgccca gcaatcacag cgcgatcgtc agcagcatgg ccgcgctgat
    18061 cggcctcaaa gagggcatcg ctcagccggc attcggcgtg gcgctgacgc tggccctcat
    18121 cgtcatcatg gacgcgcaca gcctgcgccg ccaggtgggc cggcatgccg aggccatcaa
    18181 caagatggcg cagcccgcct cggcaaacca tctcccgctg cgggaacgca tggggcattc
    18241 ccgcgtggag attgccgcgg gcatcctggt gggcattgcc gccgccctgg cggtccggca
    18301 ctttctttga acgctacccg gcagccaggg cctgttccac cgccaccagc acggcatcgc
    18361 gcgatggcgg cgcgccctgg tcgcccaggc tggcggtgta cgtcgcgccg atcagcggcg
    18421 tgcgcaccgg cgtggaggcg cgataaatgc ctatcgtcgg ccgccccagg gccgccgaca
    18481 aatgcgtcag cccgctatca aggcccacca tcaggcgggc gccggccagc aaattggcga
    18541 cggccgtcag gctcatgcgc ggcatgacct cgacgccttc caggccggcg gtcaaggctc
    18601 gcgcacgcgc ggtttccctg gcattgccgg ccagcacgcg caaggcgcaa cccgccttgc
    18661 gcacgcgctc gaacacggcc cgccagtcgt cctcgggcca gagcttgtcg tcccggctgg
    18721 ccgaaggcat gatcaccgca tagccctgcc ggtcctgttc gttgcgggcg gccgccgcaa
    18781 atccctgcag gccgaaatcc ggcgcgccgg catagtcata gccgaacgtc agcccggcca
    18841 agaggcgctg ccgcgtgacg gccggctgga gaaacgccac ccgatgccgc acgtcataga
    18901 acaaggacgc cagcgactcg cgcgccgact gccaatccag gccatgccgg gtgccctggg
    18961 cctgccgcgc cagccaggcc gacttgagca aggactgcat gtccagcacc acgtcgtagc
    19021 gcacgccgcc cagccggtcc gccacgctgc ggcgttcctg gcgggttttt gcctgccacc
    19081 aggtcttgcg ccaacggcgg tgcgcaacgg ctatcacctc ttttaccgcc gggtgccagc
    19141 cggggatttc ggcaaacgcc tcttccacca cccaatcgat ccgggcatcg ggcacatagc
    19201 gcgcgatgtc cgagatcgca ggcaaggtat ggatcaaatc gcccagcgac gaggtgcgaa
    19261 cgatgagtat tcgggtagac attgctattc agtaaaatag gccggtcata gtaactcagc
    19321 gccaaggtgc ccatgcgtgg caacaaatgg atgcgacgca tcgaccagct ggccggaccg
    19381 ctgctcctgc gcgcgctcgc cctgtttcgc cgccgcggca acccgcctcc cggcgacttg
    19441 cgcgccatcg gcatactctg cgcggtcccc ctgggagacc tgctcatctc cttgagcggc
    19501 atgatccccg cgctgcgggc aaaatttccc catgcccgga tcatcgcctt caatgccggt
    19561 ttcagccgcg gcgccttcgc gcttttgccg cgcgtcgaca agcaaatcac catcagcctg
    19621 ggcaacccgc tcggcgccgc ggccctgctg cggcaagaaa agctcgacgt gctgatcgat
    19681 ctttcgcagt gggcgaacat cacggccatc ctgtccgtgc tcagcggcgc caggcacacc
    19741 atcggcttcg acacccccca ttgcgctcga ggccgcggct acgacaccgg cgtgccgcac
    19801 ctcgataccc ggcacgagct ggccaactat gcggccctgg tcaagccgct gggcatagcg
    19861 cagatcagcg agcccgcgct gcgcctgccg caagccgaag tcgagcaggt caggcaacgc
    19921 catgccgacc ggtacatggt gttccatgcc tggcccagcg gcatcaacaa gcacctcaag
    19981 caatggcctg ccgcgcactg gcgcgcgctt gccgagaagt tcatcgccct gggctacgcc
    20041 gtcgtcttta ccggagccca gggcgaccgc gcggccacgc aggccctcat cgacggcctg
    20101 cccgcatcca gcaaggcgcg ggccatcaac gccgcgggca gcttgagcct gcgcgaaacc
    20161 gccctgctga tacaagggtc tgccggcctg gtcagcgtca acacgggcat catgcacatc
    20221 ggcgccgccc tggacgtgcc catggccgcc ctgcacggcc ccaccaaccc cctgcgctgg
    20281 ggaccgctgt cgcgcaaggc gcgggtgctc atcccgtccg gccccgacgt ggcctacctc
    20341 aacctgggtt gggagtatcc ccccggcgcc caggaaacct tgccgcgcct atccgtggac
    20401 gaagtgttcg acagcatgct ggccatgcag cggcaatcgc gccaaggcga ctgatcaata
    20461 ggcgccgtcg cgcttgaaca cgatctcgat cgtcttgaac aaaatggtga tgtcgttcca
    20521 cagcgaccag ttcttgacgt accaggcatc gaaatacacc cgggtttcgt agctgacgtc
    20581 attgcgcccg cttacctgcc atagcccggt catgccgggc ttgaccatca agtagtattc
    20641 cgcgttgtcg ccgtagcgct ccagctcttc gggcacgacc ggccgcgggc cgatcaggct
    20701 catttcgccc ttgagcacat tgaacaactg gggcagttcg tccaggctgc ttcgacgcag
    20761 aaagcgccct atgcgcgtaa tgcgcgggtc gtccttgagc ttgaagtcct gctcccattc
    20821 ggcccgggca gccggatccg ccgccagcag gcgatccagg atctcgtcgg cattgaccgc
    20881 catggagcgg aatttgtagc acttgaacaa cttgccgttc tgcccgacgc gcaggtgccc
    20941 gtagatgggc ctgccgcctt cacgcatcac caggtacgcc aggccgatca gcaagggcga
    21001 acacagcagc agcaagacga acgagctgat caggtcgaag ccgcgcttca gggtgcgcgc
    21061 cgaaagcttg gtgagcttgt tgttgatgcc gagaatcagc acctcccggc ggaagatgaa
    21121 catcatgtcg gtgctgtcca ggggcaggcc gcgcgtgctg ggcacgacgg acaccgactg
    21181 gcatttgtgc acggccaact gcttgagcca attttcacga actttttcct ggctgtattc
    21241 cagcgcgatg acatactgcg tgttgctcgc gtccgtcgca tgccacagga gttgttcatc
    21301 gcaaatgacg ggcaggccca ggagttccgc cggctcttcc tgatcggcgc agccgacaaa
    21361 atattgaatg tcgaagccca ggtaggcctc gctctgcaag gccacgaaaa cgtcaacggc
    21421 attggagccc gtgccgataa tgacgcattg cttctgccaa agcttgagcc gcctgagcag
    21481 cttcttggtc agggcccggc ccacggacag cagcaccagg atgccgcacc aggtgaacac
    21541 ccagacatag cgcgacagct gccacttgga gaacccgatg atcgccaggt cgatgaccgc
    21601 gaaaatgacg accgtcgaca agacctcttt gagctcgaac cagaaaggct tgcggtacga
    21661 gtagtggcgc aggcgcaacc agaaccagcc catgcaggcc agcgagagca ggccgtgggc
    21721 ggcgaagcgg ggagccattt cctgataggg caggaagcgc tcgaggtcgc cccagaaggc
    21781 catgaccagc aaaatggcga tgaccaggcc aagattgaag gcaagcagat ccgataccgc
    21841 caggatggcc tgcgccagga tggcctttgc gttgttccgg accgaagacg ggtgtttgaa
    21901 aagcatgaac caacagagcc tggatcgttt aggtataggt ttacagactt atcggccttg
    21961 atggctggcg cccaggacgg ttcgatagac agccatggtc ttttcagcgc aggcctgcca
    22021 gctgaattcc cgggcacgcg ccatgcccgc cacccgcaat tgctcgacca aacctggatt
    22081 atcgatcaat tggcgcatgc cttgcgccat ctcgtccgca tcttccggat tgaccaggat
    22141 agccgcctcc ccggccacct ccggcagaga ggtcgtattg gaagcgatga ccggcaaccc
    22201 cgaggcaaaa gcctcgacca ccggcaggcc aaaaccctcc gacagagaag gaaacaccag
    22261 ggcaaacgcc gtttgcatca gcacccgcgc ctcgacatcg ggcaggtact gcatccatcg
    22321 cgcttcgccg ctcgcgcgca gcgcctccac ccgcgccacc agttcctcgc atttccagcc
    22381 cgcccgtccc acgatcacca agggaaagtc ggcgcgcagc ctggcgggca gcctggcatg
    22441 ggcatccagc aggcgttcga gatttttcct gggttgcagc gtgccgacaa acaggaaaaa
    22501 tcgctcaggc agccccagcc gctccagcac ctcggccttg gtttcggcat cgaagctttc
    22561 gaagaagcgc ggatgcacgc ccagcggcgt gactgaaatt ttgtccgcgt cgatcccgaa
    22621 gtaccggatg atctcttgct tcgagtactc ggaaatggtg atgacgtgat ccgcccaatg
    22681 ccccgaccgc cgccagagcc agttcttcac cgagatcaga cgcgtgcgca cccactccgg
    22741 gttcgacagc gggatggcat ccatcagggt ggccaccacc gggatccggg aaaacttcgg
    22801 gatcaagtga tcggtggcgt ggaacaagtc gatgcgatct tgcagggctc gctcggcctg
    22861 gcatgccagg ggagtgaagg ctcccaacaa ggccatggtg gagaaacggc ccaagtcggc
    22921 gggcatctgc gcgcccggca gcgtctcggc agtcgcgtat gcctgaaaac ctgccgggat
    22981 caacgccata tcgcccgtcg acaacaaggc gccgcccagt tcccgcgtgt atgaaccaat
    23041 gccatccaca ccgccacgcg ccaggccatg cgccaagccc gttgtgccaa agccgactct
    23101 cacccaaact cctctaccgc aacttcggcc aaggcaaagc ttggccgatc caatgagggc
    23161 tatattttac cgtcggccca cgtacgccca tcccgcaacg ggcaagcccg cggctatttc
    23221 tccgcccaca ccgaatattg ggcgcccagc ggcaggcgag tcatgtattt ttccaagccg
    23281 cgcagcccgg caaacatgcg cgggaaaaag attcgatatc ggacttcaac tttcctgaat
    23341 cccgccagca ggcaagcacg tttcattttc caggccgtga tcagcctggc attgacgtcg
    23401 aattcgcaag tattgaccgc ccgaaccgtc aatggattga gcggattatg ttcgaaaatc
    23461 acgaacaccc ctcctggaga aagcgtgcgc gaaatttcct ccatcaaagg tacgtgcatt
    23521 tcatgatcaa tatgatgaaa tacgcacatg gcataagcga ggtcgaaata cccgcttggg
    23581 aacggcaaat gctctccatc gaacgatgtg aactttgcag ccccgggaaa ccgctcggcc
    23641 gcgacgccca aggaccgggc ggacacgtcg gcgcaagtca attccgaatc cgccaggtat
    23701 ttcttgatcc atggaatcga attgccgact ccagcgccaa agtcgagaat tcgttcggat
    23761 tgcttttcag gccgccgtat cagttcctgc gcaatatcct tgattttgta ttccgcgaaa
    23821 taatccggcc cctctcccga tgcggcgata tttttggcat gcatcgcccg atactcatcg
    23881 gcaaaactat cgaattccgc tttattcatg tgagatcacc ctacattcag ttggggtcag
    23941 gaaaagagac catggccgcc cgatgcacgc tcattctgcc ttttcccgcg aaataggtgc
    24001 gcaactcgaa attcttcacg ctctcggcac ggacaaaatc aagctccagt ttccgcattt
    24061 gaccatgcgt attgggcaag ccgcctttcg aggtaacgac gatttggttg tcccgcagtt
    24121 cggtggaatc ccaccagttg tcgtcggtat cggacgcata ccataacgtc actttgtaat
    24181 gtcccggcaa aatctgagcg taagggccgt aagttaaaaa tccttctttg tccttgccgt
    24241 catctgccac ccgatcggca ccgtccacat ggccaacgag gcccggtagt tctttcgcgt
    24301 ccctattcca tgcaaccaat cggccgtgca gcggcacttc gagaaactgc acataatatt
    24361 tccccttggc atcctcaaaa acccgggact ctgcttttcg aagatcgccc acgcattttt
    24421 cctgaaaccg tgcaagctcg tcgtctccaa cgggttgggt ggacagaatc acaaccagat
    24481 caccggctgc caacccgccg ccgtccggtt gcgcgcagtt ggggaaatcc atgccgcgtt
    24541 gccaatgcgc ccgttcggta aaggaagact cgatagagac aaaataagta tcgccgactc
    24601 ttttttctgc gcgggtttcc tccgaccaga aaattttcgg cagtcggcct tgggcttttt
    24661 caacaatgta ttcatgaatc ggatagataa ttggccgacc atcgaactcg tgcgggataa
    24721 gcagtgttgc aacacctacc agcaaagcca atcccagtgc catccatcgc cgcgccattt
    24781 cttcgaagaa aaacgccagg aaacagagaa cgccgattaa agcggcgaca gcccataccg
    24841 gcaattctac cgatccgata gcaagccgga tccacagaga aatgacgcat acggtaaaaa
    24901 cacccagtgc cgcccacata tttaacgatt ttttgtggaa taacagcgcc gctatagtca
    24961 aataggtaag cgcaaaaagc ggcgttgcat acaagccatc ccggctgtaa agaagcaaca
    25021 atccatcaag cttgaagatc gccaacaggc cgcagaacaa cggtccgata acaatccaga
    25081 aaaaaatatc cggctccggc atttttctgt tcacgcacat ccagaccacc gccatcgatg
    25141 ccccgaacaa ggccgcccag tgtattgtca cccaataaga cgacgtgcct agtgaaccaa
    25201 aagacaaagc ccagcttgac gcatgatcca gaaaaagcat taggatctga ggtttcagaa
    25261 agaaagtagt gtcgtgagtt ccgtagtaaa aaacgccggc ccatactaca aatgcaacga
    25321 tcacgccacc gattccatac gacagcgtgg gacccaataa ttttatgaac ctgactggct
    25381 cttgcctggt gacgaaaaac aaatagtaaa gcacgagcag cggtaaataa accagggcaa
    25441 ccagatgggt aaacaccatg gatgccgcgc agaatcccgc gagaatgaga tagagattac
    25501 gatgaactga ctttaccgcc cttgcgagac agacgaaggc gagcatttga tataagagca
    25561 ccccggtatt gacgtaatcc gatccggcat ccctgataaa aaaaatgtcg gtgcccatca
    25621 tcgaggccgc cgcaaatgcc acgcctcttg aagtgtggac tcgcgccacg taccagaagc
    25681 acaggatgat taccggatat aaaaacaggg catgaaacgc ataccgggca gcatcaggat
    25741 tcagaaaatg ccaaaaaata tgcccaagac cgatatatgg aacccgggtt ccataatatt
    25801 tatgggcctc gtctatagaa aaataattcc aaaactcaaa gaaatgccga aaatatccat
    25861 aatagaacca gacatccaga tctgttttgt caactatcca tagcgggtag ctccaccaca
    25921 agactgtggg gaatatcaac agcagccctt tctcccaatg atcggcaacg agtctttgaa
    25981 tacttggcgg catcaattag gctccatcga cattgccaaa tatttgacgc gaccatgggt
    26041 ggggtgcgac ttcatgatga tcgacttggt gacgttaagc cccgcgttcg cagcaagcgc
    26101 ttcatagccg cgctgctttc tgcaataccg gccacgatca caccaggcga aagcgttcga
    26161 cacccactcg ccgggcaaat acacaatatc caggctgact atcctgcgca atctggggga
    26221 tttcgccagc atggacaaca aatccatggc ttgctcatcc gtaagatgat gcaatagccc
    26281 cgccaggact acgcgggtgg gctggatttt ttcgacatcc tcggtttgaa acaacctcgc
    26341 gtgaaagctc gcattcgcta ccgaggcaaa ccgtttcgat gcgtaggatg tggcgacctc
    26401 atccacgtcg aaacccaggt actccttgaa gctcttcaaa taattcagtg catcccctgt
    26461 gccgcacccc acatcgacga ccacgtcctg ggcagatacg tccagcgact catataaatt
    26521 cgaaagatct atcccgccaa cgatcaaagg ccggattttg ttatatatga tgggatgacc
    26581 caggatgcga ttgtataggc tcatgataat aataatgatt agatggagag gattactcgc
    26641 ggcatttctt gtgacgccca gtcatgcggc gtatgtccat tttctattgg caaaaaagtt
    26701 ccatagaata acgcctcctg tggcacagat ttgcgcatag atataattca gcatgaacac
    26761 ggcggtgccg atcttcatga acaagccatt catcgcaagc ccgatcagcg caatgaccca
    26821 gaatcgccat aatgccctgc cgtggctgat gttggcctgg aatgtaaatc gataattcag
    26881 gaaataattg agcatcgagc aaatgacata ggcgatgcaa gacgcatagg tggcgtcgat
    26941 gccgatgcgc tcgacaaaaa gaaacagaag aacaaactgg acaaccgcaa ccacgctgcc
    27001 gaccagcagg aatttgatga gttgccgaat cagcacggcg tcaagtttgc ctgaaacaaa
    27061 gaccttcgct cgacggatga catccattat cggctcttga taatgacgac gggactgcgg
    27121 ctgctctcaa gctcggaggg agcgaagccc acatactctc ccaccacgta taaaggcctg
    27181 tgcttgcttt cgccgtatat tctgccgata tattcaccca gtatcccgat ggaaatcagc
    27241 tgaacaccgc caatgaacag tacggccacc atcagggcgg accacccttc gacccaattg
    27301 gaagtgaaca ggcgtatggc caggacatag atcaaaccgg ccaaagccaa taccgccgag
    27361 cagatgccca agccgattga aaactgcaaa ggcttgcatg aaaacgacat gattccatcg
    27421 gtcgcgaaac gcagcatttt tctcagcgga tacttgcttt cacccgcgaa acgctcggcg
    27481 cgccggtaag gcagcgcgac ctgattgaag cccgcccagc tgaccatgcc ccggataaaa
    27541 cgatcccgtt ccggcatggc cttgaggatc tcgaccacag ggcgactcat caacctgaaa
    27601 tctcccgtat ccagagggat gggcacatcc gacaatttgt tcagcagccg gtaaaaagtg
    27661 cgggccgttg ccagcttgaa ggccgactcg cccgggcgtt cggttcttgt gccgtaaacc
    27721 acgtcatacc cttcacgcca tttttcaacc atctgcagca cgacttccgg cggatcctgg
    27781 agatcggcat cgatcagcac aacggcatct cccgatgccg catcgatgcc ggccgttgcc
    27841 gcgagctgat gtccgaaatt gcgggcaaaa ccgattatct ttactcgcgg gtcgtgcgcg
    27901 gcatggctct ttagcaatgc caaggtattg tcctggctgc cgtcgttcac aaagatcagt
    27961 tccgccgcca gattttccaa ttggtcacag agcgcgccaa gacgctgcat cgtttctccg
    28021 atgacctctt cttcgttgta gcaagggatg acaaatgata ttttttgcat tttcaataag
    28081 ttactatcaa agccaattgt tacaatctaa gcgacgattt taaagcccct tcagtcgttc
    28141 gccacaattg ctgcacattt gactgaagca aatcgtgcag cgaagactct atatcctcac
    28201 atactggtat atgtgaggag ccgtattact gcaccgcctg ccgcagccac ttgaggctgt
    28261 tcttgtgccc cagagatccg tttgcaagcc aataccgagg aggttgatgc aacagctcaa
    28321 ccgggtattg ggtaaagccg cctgcaagat cgtttcgcac gaaaaatgcg ttggcaccgc
    28381 ttgcattgca tgcaaccagc gtatagcccg gcaacgcgtc gatccaggcc tgcagcgaag
    28441 caccgtaata atcatccccc atccactcgt gcgccgcgtt gtattccatg accatgtggc
    28501 tgggcggcgg gaatttgccg ttgtactcca cgcagatgag cttgggggag atgacatcga
    28561 tcgccacctt catcaggtgc cagtcattgc catccacgtc gagcgaaaag aaatcgactg
    28621 ccttttcgtg caggaaagag gccgcctcct ggatcaactg agagatattg cccaaatcga
    28681 caaagctgtt cttgatcagc aggcgctcga actgcaggcc gccgagctgc tcgctcatga
    28741 aagctgcttt ttcttgactg ccttccaccc agacgccttt gtagccgtcc aggacaagct
    28801 gatgcgtgtt gttttccaag ccgtccgcgc agccgatttc gatgaacgtc ctggaacatg
    28861 ccgtgacctg ggcgattcgg cgcaggcatt ccctgatgat gccgtcctca tcgcattggg
    28921 aaaagctcgc ccagccatgg accaggggat tatcgggcat cagcgccttg agctgttcgc
    28981 gaaccaggca caggtctttg cttatttcca gttgatcgac atggtacata tttgaagcct
    29041 taacaataaa ttatggcaag cggcatgccg gaacagctgc ggtctttagt ccagcttata
    29101 gttggacgcc gaaaaaggca tataaaccag cccccacaaa tcatgctcgc actccgcctc
    29161 ggggaactgg acactgaatc ttgccccgcc tgcagcgtaa tcaatgatgc tgtttgcaat
    29221 catttgccca tgatttctcg gattcttcat cagcccccct atccccgcta tcagtccgta
    29281 ctctccagga cacaaagggt tggcgaattc gaattccatc atttgcagga tgttgcgctc
    29341 attcgcatcc agcgggccag cctgggcgat ggagttgaaa tgcatgacct gcttgccact
    29401 gctatccgtg acgcccaggg tcaggccgca gttttccggc aaagggcctt caatcttgta
    29461 aaaaacccgg atccgggcct tgctgttgaa cgcaatggta tccgtatcct ggccgttttc
    29521 gccaatcaag gcgatgtcca ggatccgggc ctcgccgctt cccgtgcgca aggacgatac
    29581 ccgctcttcg aatgccttgt gacggagatg gtcgatagca taggcccgtc ccccgtcctg
    29641 atcggcttca atggccttgg gctgttcatc ctgctcggtc tcggccaatc cggcatgcac
    29701 gacccgccgg tagatggcgg actgataggc ggcccccacc tcgctggaag cgcccgccag
    29761 gcgcacgcgt ccatgatcca tccatacccc tcgatcgcat agcgtgcgta tggagtcggc
    29821 cacatgcgtc acatagatga acgtgacgcc gtcatcgagc agcgacttga tcttgtgcag
    29881 gcatttgttc tgaaaatagg cgtctcctac gctcaaggcc tcgtcgacga tcaggatttc
    29941 gggcttcacg tacacggcgc aggcaaaacc caggcgcacg gccatgccgc tgctgtaggt
    30001 cttgaccggc tggttcaccg cgtcccgcaa ttcgctgaac ttgatgatgt ccggcatggc
    30061 ctcgtcgacc tcttcggccg acatgccgtg cagcatgcag aattgcttga tgttttcctt
    30121 gccgggcagg aggggattga accccgcacc caattccagg agcgccgaga cacggccgtt
    30181 caccgtgatg gagcccgagg tcggcgtcag cacgccggcc aataccttga gcagcgtgct
    30241 cttgccgctg ccgttttctc cgactatgcc cagacggcca cccttgggca gcgagaagga
    30301 caccgagtcc agcgccttgc tctccttggc atagcgcttc ttgccacccg agattatttc
    30361 cagcagccgc tgcttcggcg cggcataggt gtagtacgtt tttgttatgt cgacaagctc
    30421 gattgccttg tcattgtccg ttgccgtcat cattcgatca cccgatcaaa gcacatccgc
    30481 gaagccggat ttcagcttct tgaagatgag cgccccgatt attgcaacaa tgatcgagag
    30541 taaaagcaga atcccggcgc ctagcaatgt gatggcggac tcggcgccga atatggcact
    30601 ccgggaaaac tcggcccaat aaaaaaacgg gttgaaatcc atgagcagag ccttggccac
    30661 ggggcctcca tgctcttgca ccaaggcaac cggatacagg atcggcgtca tccagaacat
    30721 gatgttgacg gctaccggca cggcttgcga caggtcgggt atataaaccg ccaaggcgga
    30781 aaccatatag gccgtgccga ttgtgaaaat gacgcacatc agccacacaa acaagaagcc
    30841 ccaggcatgc gtagtaagcg ccatcccatg ataggccatc agcatcaagg taatggcgat
    30901 aatcaccgcc tggctcagca acgccgacgc catgggcacc aaaggcagca cccagaatgg
    30961 aaacgagatt ttattgacca gatagctgta ctcgcggagg atccccgtac tgcggttgac
    31021 ggtttccgaa aaaaagatcc acagtgaaaa cggcacaaaa tagaaaagcg agaagggcac
    31081 attcgcatac ccttctccca tgcggccgcg taccaggatc gagaacacaa ccacgttgac
    31141 cagcgcgtaa agcagcggaa cgcctatgtt ccacgccatg cccgcgtagg agccggcata
    31201 ccggttcttc aaatcccgct tggccagcat ccaggccaaa gacaaacctt cgaggaaact
    31261 tacattattc atatgagcct ttcgcgggta tccaaaggcc gcaccatact atcgacgaac
    31321 cgcaggaaat ccgcctcgat tgtcttggga tggaatctgg caacatgcct gcctgcttga
    31381 tcaataaaag tgcgatattt cgacccattc gtccaggccg gctccccgat ctgggacaac
    31441 acatcccgca tagtcttggc caaaacgcca atatccccgg taggcaccaa atcggccaac
    31501 tcgccggaaa taaacggaac attgctgttg tcataggcaa ccacgcggca accggacgcc
    31561 aaagcctcca gaatcggaac gcagaatcct tcgtggtagg tgggcagcac gaataaatcc
    31621 gcatcggcaa gcaccgcctg cttttccgat tccttcgcat ttcccctaag agtgatagcc
    31681 agcctgccgt gaaaatcctg acgcagcctt tgcattaaag actgcaacgc atcgaggacc
    31741 accggatcgg aaaaatccag gtttccaacc agatcaagct gaattttcgc ctgcgcatca
    31801 tccgccatga cgcggcgtat cgcttcaaga agctcgatga cccccttgga cctcacgaat
    31861 cgaccgacaa aagctatccg agacacgttg tcggaaaaac tgggcttagc cactggagcc
    31921 tgcaggttgt tccgtattgc cagcggaagc gtgatgctcg gtatgtcgat accagcggcg
    31981 gacagtacct ctttattcac gtcgctatca catacaacct gatccgcgac agcaatattg
    32041 ctcatctggc tgaacgagag atcaatatcg gcatgcgccg attccggcac aaactgcttc
    32101 ggcgttatgt tgtggaagac caccactttt ttggctcggg tcggcgcaat gaacagcagg
    32161 ttgaaaagcg gataatgcac gccgaaatgc cagacgatca gatcgctgtg ccgatagtgc
    32221 gggtcataca tgacatcggc caatccgccc acttgcttgt atggcagatc tttgtaatca
    32281 cagcgataag tgaaaagccg gacgtgatag cccgccttcg tcagccattg aatctcttcg
    32341 cgaatcgaat tcgagatggc atcgtgctct acgcacacac cgctgacaaa agagacgttc
    32401 actggcatcg gttcccgaaa tttgccatgg ctcgggcaat cagctccacg ctgcgctgcg
    32461 ccattgcctc cgatgtgaag gaggtcagaa aacgcatcct gccgcgttcg cccatttgac
    32521 ggcgcttttc cttggagccg gccaacacca tcagcgcctg ccccagtgcc gcggcatcgc
    32581 ccggcggaac cagatatcca ctttggccgt cttcgatgag ttccggcatg ccgcctgccg
    32641 cgcaccccac aacgggcttg cccgcgcgca tggcctcaag gaagatcaag ccaaaagact
    32701 cgaacctgct gggcgccaca aacacatcgc aggcttcgta tgccgccaat agctgggcat
    32761 cggagactcg cccgtcaaaa tgcacacgac cgatcagcgt cttgtccaat gccgatcgca
    32821 aaaacgtctc tttatacgtt ttgcgctggc cagggatggg caaggtatcg tcccccacga
    32881 tgcgcagtgt cgcattggga aattgccgca gcacctcggg aatcgcctcg agcaggacat
    32941 cgataccctt gcgcggctca agccggccaa caaaaagtat ttccacggca tcatccgacg
    33001 ccggcttgcg ctgcgatgga gccagatccg gcatgcccag atgcgcaatg cacacccgct
    33061 catcgggaat ggaacagcca tacgccgctt cgatgtcttt ccgtatggcg ctgcttatgg
    33121 acctaagcag gtcggactgg cctatcaatt cacgttccag ggcaagcatc ggcgtgccat
    33181 gcgtgcgcat ccaatcggga ttgctttcca gttctggatg atgatcgaga aaaaacctca
    33241 acgtggtctg caggctcgtg accagcggcc attggcgcgc caaaagaaac gcgatccctt
    33301 cgcaatccca gatcggcgct tcgaccacat ccaccggatg gtgcgtgcct atgcgctcga
    33361 cttcttcgaa tgccgttgcc gaccaattcc agatggattg cggcacattt cgcgcaacgg
    33421 cggcttcgct gcgagggata tcccgcgacg ccatgcgatg cacccaaacc ccgttttcga
    33481 aatcgactcg atttatatcc gggctttgcg taatcacgtg caccaggttt cccttggccg
    33541 ccagcgcctc cgccagatct ttgttgaatg tggcgattcc gccattgtgg ccgggaggaa
    33601 agtcccggga aacaaacacc agggttttga actccggatt ctggataggc gtgaaaggca
    33661 ggaatgttcc cgcgtatttc gctcgcttgc tcggcgtgat cattgcgtcg ggcagaacgc
    33721 cctcgaaacc acgcttcgat ccgacttcga tggccttgac cagatcctgg gaaaatcgct
    33781 cagcatcggc actggaaata aagccgttac cgtgatcccg atcaacctcg tcttgctggc
    33841 gttgaacaaa ggcatcccgc tcctggatga tccgatccag cggatggaat tcccgggcat
    33901 gcttgagcat gaagtagatt ttgtttttta tgaccggata acgatagcgc accactttat
    33961 tgtcgccccg gatgttgctg ggcgcatatt tatgatgcac gaacgcatta ggcaactgct
    34021 ctatcagata gccggcgtca atgatgcgaa ggcatacatc cgtttcatcg agaaaatact
    34081 catactcctc gtcgaacccc ccgattttca acaatgccga gcgccgaaaa gatgaattgc
    34141 atcccaacaa atgcgggaag tggccgcttt tcggaaaaga caaatggggg aaagccctgg
    34201 agggcgaaag atcggcattg ccgaagcggt cgaccaggca atatttgtat tggtattcat
    34261 agcctgtgtg attgaacaca aagccgccaa cggccccgac ctgaggatct cggtaagccg
    34321 ccgccatctg ctccagccac tccggctccg gcacggcatc atcatcgatg aaggccacaa
    34381 tgtcgccccg agccatgcag atgccgacat tgcgcgaaac ggagagattg gcgacatcgc
    34441 acctccccgc ccggatgcgc ggcagccacg acgcgatgat ctcatccgag ttgtcggtcg
    34501 aaggaccatt gacgacgaca acctcgaatt gacccgaata cttcagccat tggaagcttt
    34561 ccaatgtctt ttgaagcatc gggcctcgat tcagcgtatt gatgacaatc gtgaaggaag
    34621 ggcttgtatc gtgcgcggcc attctgttca cttttttgcg actatctcga attcatagga
    34681 tttgccattt ctacggcctg cctcgatgac cgagacatct ttgaaaccgg cttcttgcag
    34741 caacccggtc aacgaatccg gcatgaacat gttgaagtga aaatcgccgt catagtcctg
    34801 gcctccatac agtacttccc gcatgtcgtc gtaagggtag ttgccgttgg agaactcccg
    34861 catcatggct tgagcgtcgg gcactacagc atggaacatg ccgccctctt tcaataattt
    34921 gtggaaatac ggcagcaatt cgcgccgcaa ttgttcctgc gggaaatgtt ccagcaaatg
    34981 cgcggagaag acctcatcca attcgttttc gccaaatggc agctcgttga tttcggaaac
    35041 gatatccacg ccgggcaacg cgcgccgatc cacattcaga tagccatcga gcggcacatg
    35101 gccgcagccc agattcaggc acagcctgcc ggcccgggcc tgggccagtt tttcctggtt
    35161 caatacttcg gttgtggcct tgagttggtc ttcaccttcg gcggcaggcg tcgaggcgcc
    35221 ataacgcatc tcgaacatca actcacgacg aacgaactcc acccggccca acagataagc
    35281 aatggattcc cgtgcttcgg cgatggcctg cgcattctga cgcaatgaga caggtacgga
    35341 ttggatgaag ttgtcatgct cgaggcgccg atggttcata tccgaaattg cctgcagcag
    35401 cagcggcagc tgttcattct ggaatacccg ttgacgctcc agattgacag gcaaccggac
    35461 aacggcgata gccatgcgca cgaccgggcc aataacaggc attctcgcca gcctacgtat
    35521 tatttttttc acgaacgatt tcattttttc cctttcaaca gccatttgga tttgtcgtct
    35581 gccaccgcca ggtatcctcg cacatgcgtt cgatgccgta ttgggcttcc cagcctagaa
    35641 tctgctttgc caatgccggg tccgcgtagt attcggccag atccccagct cgccgggcca
    35701 cgatcttgta gggaatggat tggcccgaca ccttgccgaa ggccgccacg atctccagta
    35761 cgctggtcga ccttccgccg cccaggttga cagtgatggg cgcatccgac ttggccaggg
    35821 cagtcaacgc agccagatgg ccggccgcca ggtcttccac atggatatag tcacgccgcc
    35881 cggtaccgtc cggtgtcgga tagtctccgc caaataccga cagaaactca cgcttgccca
    35941 cggccacctg cgcaatgaaa ggcatcaggt tgttgggaat ccccatgggg tcttcgccaa
    36001 tgaggcctga tgcatgggca cccacagggt tgaaatagcg caggatggcc acgcgccagc
    36061 cagcgtccgc cttgcaggta tcgcgcaata tctgttcgac aagaaacttg gtccggccat
    36121 acacgttcac cggcgccagc ggcgtatctt cgcgatagtt tggataaccc ggatcgccgt
    36181 acaccgtagc cgacgatgaa aacacaatgg tcttgatgcc agctcgggcc atctcctgga
    36241 acaggaccag gctgccagcc acattgttgt cgtaatagac cagcggcttg gcctcgccct
    36301 cgcccacggc tttcaggccg gcgaagtgaa cgaccgaatc gatctcgtgg gcatcgaaca
    36361 catggcgtac tgcttgcggg tcgcgtacat cgccctggat gaaagccgga cgccggccgg
    36421 cgatgcgctc gatacgatcg agtacgccca atttgctatt gcttaaattg tcaagaatga
    36481 caatttcacg cccggcgcca agcaattgca cgaccgtgtg cgagccgatg tagcccatgc
    36541 cgccagtgac caataccgcc ttcgccaaaa cctgctccta tattgatttg ctcgatagcc
    36601 gccggagccc atgcgacggc cctgaatcgg cttgtaccaa accctgcttt accagcgccc
    36661 ttcgtcgaac atccagcgca gcgtttcttg caaggggatc tgccgcaagg cgcccacggc
    36721 ccggatcaaa ttgcctgcat cgccttgcag gcgcttgact tcgttggcac ggacgaatgc
    36781 cggattgacg cggatctcta tctggtaccc ggcgatctct tccatcgccg caatgacggc
    36841 tttcagcgac acggccttgc cggaacagac attgtagatg ccgcccgccg gcgccacgtc
    36901 gagcagccgc atgtaggcgg cggccaccat ccgcacgtcc gagaaatcgc gttccacgtc
    36961 gatattgccc agttcgatca cccgctcgcc acgctggaaa tggctgacga tcttgggcaa
    37021 caggaactgc gccgcctggc ccacgcccgt atagttgaag ggcctggtca tgacgatggg
    37081 cagcctgttc atccacagcc tggccatgta ctccatggac agcttgctga cggcgtaatc
    37141 gttggccggg ttggccgcga cagattcgtc gatgacttcg accgccgcat tgccgtaaat
    37201 gttggcgctg ctggccaaca gcaccgcgcg cggcggcctg ggctggcggg ccagggcctc
    37261 cagcaggtta taggtgccga ccacattggt gcgatagatg gcatccacat cgccatgcgc
    37321 cacaaacgat atggcagcca gatgcgccac gatatcgggc tggatctgcg ccacggcctg
    37381 cgccaatgcc tcgcgatcgc aaagatcgac attgaccatg ccaggcccgg tccggtcgtc
    37441 gccataggtc gtgccgaaca cgcgatagcc ggcggcttcg agctcttgcg cgagatagcg
    37501 cccggtaaac ccccccagcc ccgtcaccag ggcccgggga gcgtcagaac gagcagccgg
    37561 tttcattacg gcgcagatcc gcttcgacca tcatcgaaca cagttgctca agtttggtct
    37621 tgggctccca gcccagtttc tgcttggctt tttctggatt gccgatcagc agatccactt
    37681 cggccggccg ataaaacttg gggttgacct tgaccagcgt ccggcctgtc gcggcatcga
    37741 tgccgacctc gctttcgtcc ttgccctgga aatccagctc gacgccggtc gccctgaatg
    37801 ccatgcgcac gaagtcgcgc acggtttccg tgcgattggt ggccagcacg taggtatcgg
    37861 gcgcgtcggc ctgcagcatg cgccacatgc cctctacgta ctccttggcg aaaccccaat
    37921 cgcgcttggc atccatgttg cccagttcga gcacgtcaag cttgtcgagt ttgatcttgg
    37981 ccaccgagtc ggtgatcttg cgcgtgacga attcgcggcc gcgcagcggc gattcgtggt
    38041 tgaacagaat gccgctgcag ccgaaaatgt cgtagctttc gcggtagttg atcgtcatcc
    38101 agtgcgcata cagcttggcc acgccataag ggctgcgcgg atagaaaggc gtggattcgg
    38161 tctgcgggat ggcctgcacc ttgccgaaca tctcggacgt gctggcctgg tagaagcggg
    38221 ccttggggtt gacgatgcgg atggcttcca gcaggttcac cgcgccgagg cccgtgattt
    38281 ccgccgtcgt cagcggctgg tcgaacgaca cgcccacgaa gctctgcgcg gccaggttgt
    38341 agacctcggc ggcgcccgtc gtctgcagca gacggatgct ggacgccaag tcggtgagat
    38401 cgtattcgac cagatgcagg ttgggatgat tctggatgcc cagctcttca atgcgccaga
    38461 aattgaccga actggtgcgg cgataagtgc cgtagaccgt gtagcccttt tcaagcaaaa
    38521 gctgggccaa ataggcgcca tcttggccag aaataccggt gacgatcgaa gttttcatgg
    38581 ttttagggat ttaatggatt gaaattgagc cttgcgaata aggacgcatt gccgggcgct
    38641 gtctgccgat gttttaagaa tggattcgcg cccctgcccc ggagcagccc gcttgcaaat
    38701 gggggaatca gagcggtctg ccgcccaatc aagcccgccc gtagatgtcc tggaaacgca
    38761 cgatgtcgtc ttcgcccagg tattcgccgc tttgcacctc gatcatcacc aggtccatca
    38821 cgccgggatt ttccaggcgg tgcttgtgcc cggcgggaat gtaggtggat tcgttggtgc
    38881 tgacgaagat ctcccgttcg ccgttgacca ccttggccat gccgctcacc acgatccagt
    38941 gttcgctgcg gtggtggtgc atttgcaggc tcaggctggc gccgggcttg acctcgatgc
    39001 gcttgatctt gaagcgcggg ccttcttcca gcacggtgta ggtgccccac gggcgggtta
    39061 ccgtgcggtg cagcttgtag gcttcgtggt tttgcttctt gagccgggcc acgacctgct
    39121 tgacgtcttg cgccttgtcg ggatggacca ccagcaaggc gtcgggcgtg tcgatgatca
    39181 tcaggttgtc gacacccacg gcggccacca ggcggccctc gccatgcaca taggtgttgt
    39241 ggctgtcgac gaagacggcc tcgcccgtgg cgcggttgtt ttcggcatcg ggcttggaga
    39301 gctcgcgcac cgccacccag gagccgatgt cgctccagcc gaagtcggcg ggcaccacgg
    39361 cgaccttgtc ggaacgctcc atcacggcgt agtcgatgga gatgtcgggc acctgggaga
    39421 aggcttccgg cgggatctcc agcatggcgc cgtcgtgggt catggtctcc cagcaggcct
    39481 ggacggtttg ggcgacatcg ggcgcgtgcc gaagcagttc gtcgaggatg gtgcctgcct
    39541 tgaagcagaa catgcccgaa ttccagtaga aattgccggc ctggaggtat tcgcgggctt
    39601 tgtcgaggct gggtttttcg acgaagcgca gcacgcgctt gccctcgccc agggcttcgc
    39661 ctgcctcgat atagccgaaa ccggtttccg gggcgctggg aacgatgccg aaggtggcca
    39721 gatagccttg ccgggccagg ctgaccgcgg cgtcgacggc ctgcacgaaa cggcccatgt
    39781 cctggatgag gtggtcggcc gacagcacca gcatcagggc gtcgcggcca tgcttttcgg
    39841 ctacgcagtg cgcggccagg gccacggccg gggccgtatt gcggccttcg ggctcgagca
    39901 ggaaggtgct ggactggccc accttggcca ggccgagctc gtccttgctc atgaagtagt
    39961 agtcgcggtt ggtgaccgtg aggatctcgc cggcctcgcc cgccggcttg ccggctgccg
    40021 cggcggcgcg gcgatatgtc ttgagcagca ggctttcgcc gtcggccagc ttcatgaagg
    40081 gcttgggata gccttcacgg gaaaccggcc agagacgggt tccggcgccg ccggaaagaa
    40141 tgacggggat cagcatgatg agtctggtca ccaaggtgca acgtaaatgt aggtggccga
    40201 ttttaccttg tccccctctg gcctatcgcc tcaggtgaga caattggcta acttttgata
    40261 cgttctccct atggtgggaa cggcataagg ggtggaaaat gggtccgtag tctgttgtcg
    40321 catgcacaca aaatccataa ggagacaatc atgcgtatag ctgatagcca gtggattccc
    40381 tccactcccc atcagacctg ggacgccctg ctcgacccgc aggtgctgca gcaatgcctg
    40441 ccgggctgca tccaggccac ccaactgagc cccaccgaat acgccctgac cctgcgcacc
    40501 aagatcggcg ggctggaatg cgattaccag ggcgaaatcc tgctgtcgga cgtcgaaccc
    40561 gcccgcagcg ccacgcttgc cttcgaaggc aagggcatgg ccgccgggct ggccatcggc
    40621 acggcccagg tgacgctgac cgagaaggaa tatggcacgc ggctgagcta caccgtggcc
    40681 ggcatggccg gcggcaagct ggccgagctg ggcgaggcga ctgtcctgaa ggcgggcggc
    40741 aagatcgtgg acaagttctt cacggcgttc gtcgagtaca tggctcgcca gccccgcacc
    40801 gctgcgccgc ccgccccggt cgagcccgag gcgaccggca tgtcgaattc gccatggtcg
    40861 tgggccgtgg cggccggcgt ggtg
//
LOCUS       DASWPO010000041         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1078936, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000041 DASWPO010000000
VERSION     DASWPO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078936"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VGC69_02380"
     CDS             complement(<1..529)
                     /locus_tag="VGC69_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294164.1"
                     /translation="MSVEIRNVSKSFRSTLAVNDASLSLADGSFVSIVGPSGCGKSTL
                     LRLVAGLMPPSSGSITVHGKPVKGPLRHIGMVFQAPALLPWRRTLDNILFVAEMGGLK
                     ASDWRDRALELMQLAGLGGFEKSYPHELSGGMQQRASICRSMLLKQPLILMDEPFGAL
                     DVITRERMGYALQTLW"
     gene            complement(591..1580)
                     /locus_tag="VGC69_02385"
     CDS             complement(591..1580)
                     /locus_tag="VGC69_02385"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294165.1"
                     /translation="MKRIKYFAAALAAWVVVLAAPAAAQAPAPKKITFLTNYTFNGRH
                     APFFVGLEKGFYKEAGFDVTIAPATGSGFVIAAVDGGKADYGMADFGSVTQSVAKGAK
                     IKAFTVYADVTTNGLASLAPVSAPADVIGKKVAAGQTDSVRVILPIIFDAKKLDASKI
                     DWQSADPSVYFSLLLSGQVDMITATSDGDMPSLTKAASQQGKKVNFAYFADWGYDVFG
                     FVLIGSRDAIAKDPSDAARFAAATKKAVEYSIAHPDEAAAIMVKYNPTLSLDTVKAQL
                     AGALQAIQTPYVAKNGYGVATEERLAHSIELVKTALNLTANVTPADVFTQLGK"
     gene            1735..2574
                     /locus_tag="VGC69_02390"
     CDS             1735..2574
                     /locus_tag="VGC69_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0294166.1"
                     /translation="MGDLIRNQGAARAGGEDPPRSGTINSVEHAIELLRCLAVAGGPL
                     GTNDLARRIGLHKSSVSRLAATLEKARLLRRDPATGRFLLGLGLLALAAPLFADFAVA
                     DIVEPILVKLAAETGETCSFNIWDGQDAVSIAQVPGSHSVRAFSLPGQRKPAHATATG
                     KILLAHLDEAALSAYCSTPLRRYTDTTITDSKALVENLVQARQRRYAINLGEYESDVG
                     GIAAAVFDMSGRLFGCVATTMPLYRFAPERHAALGAAVSACATEIAEHLGIYRRKGRE
                     GPP"
     gene            2571..3164
                     /locus_tag="VGC69_02395"
     CDS             2571..3164
                     /locus_tag="VGC69_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HEY0294167.1"
                     /translation="MIACTRLSIDGALWSYAERLYACPGVAGACLGLQDRAGVDVALL
                     IAALYGAGRGCVVDGDFLQRLDAVSSALRREAVGPLRAARRRLKAHPLTARFAEVADF
                     RERIKAVELRGEQVQLALMAQVIEQALEPALEKTAPATPAAPIGRDAYRRAGMAVLRH
                     FTQGQAPERDEDAAAIQDIADAAAGAAAPDGRELPMP"
     gene            3363..>4348
                     /locus_tag="VGC69_02400"
     CDS             3363..>4348
                     /locus_tag="VGC69_02400"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY0294168.1"
                     /translation="MSHNTDDLRIREIKELNPPAHVMRELAATPVVSATVYEARRGLH
                     RILHGMDDRLIVVVGPCSIHDTKAALEYARRLKAEHDRLAADLEIVMRVYFEKPRTTV
                     GWKGLINDPDLDGSFNINKGVRVARALLLEINSLGMSAGCEFLDMITPQYIADLVSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNVRIAVDAIKAASQPHHFLSVTKGGHS
                     AIVSTTGNEDCHVILRGGKAPNYDAASVDAACQEMAKSGLAQRIMIDASHANSSKNPE
                     NQPLVMEDVAQQIERGEARIAGVMIESHLVAGRQERVPGQPLVYGQSITDGC"
BASE COUNT          732 a         1401 c         1473 g          742 t
ORIGIN      
        1 cccagagggt ctgcaaggcg tatcccatgc gctcgcgggt gatgacgtcg agcgcgccga
       61 acggctcgtc catcaggatc agcggctgct tgagcagcat ggagcggcag atggaagcgc
      121 gctgctgcat gccgccggag agctcgtgcg ggtagctctt ttcaaagccg ccaagcccgg
      181 cgagctgcat gagctcgagc gcgcggtcgc gccagtccga cgccttgagc ccgcccatct
      241 cggcgacgaa gaggatgttg tccagggtgc ggcgccaggg cagcagggcc ggcgcctgga
      301 acaccatgcc gatatggcgc aagggcccct tcacgggctt gccatgcacg gtgatgctgc
      361 cgctggacgg cggcatcagg cctgccacga gtcgcagcag ggtcgacttg ccgcagccgc
      421 tggggccgac gatggagacg aagctgccgt cggcgagcga gagggatgca tcgttgaccg
      481 cgagggtcga gcgaaaactc ttggaaacat tacggatttc aaccgacatg aaaaactcgc
      541 attgcgccgg cgcggctagc gggccgccgc cggcgatcgg aacggggctg tcacttgccg
      601 agctgcgtga agacgtcggc gggcgtcacg ttggcggtga gattgagcgc ggtcttcacg
      661 agttcgatgg aatgcgccag ccgttcctcg gtcgccacgc cgtagccgtt cttggcgaca
      721 taaggcgtct ggatggcctg cagggcgccg gcgagctgcg ccttcaccgt gtcgagcgag
      781 agggtcgggt tgtacttgac catgatcgcc gcggcctcgt ccggatgggc gatggaatac
      841 tcgaccgctt tcttcgtcgc ggcggcgaag cgcgcggcgt cggacgggtc cttggcgatg
      901 gcgtcgcgcg agccgatcag cacgaagccg aatacgtcgt agccccagtc ggcgaaataa
      961 gcgaagttca ccttcttgcc ctgctggctc gcggccttgg tcagcgacgg catgtcgccg
     1021 tcggaggtgg cggtgatcat gtcgacctgg ccggagagca gcagcgagaa atagacgctc
     1081 ggatcggcgg actgccagtc gatcttggac gcatcgagct tcttggcgtc gaagatgatg
     1141 ggcaggatga cgcgcacgct gtcggtttgt cctgccgcca ctttcttgcc gatcacgtcg
     1201 gccggcgccg acacgggagc cagcgaagcg aggccgttgg tcgtgacgtc ggcgtagacg
     1261 gtgaacgcct tgatcttggc gcccttggcg acgctttggg tgaccgaacc gaaatcggcc
     1321 atgccgtaat cggccttgcc gccgtcgacc gcggcgatga cgaagccgct gccggtcgcc
     1381 ggggcgatcg tcacgtcgaa tcccgcttcc ttgtagaagc ctttctccag gccgacgaag
     1441 aacggcgcgt gccggccgtt gaaggtgtag ttcgtcagga acgtgatttt cttgggcgcc
     1501 ggcgcttgcg cggctgccgg tgcggccagg accactaccc aagccgccaa agcggcggcg
     1561 aaatacttga tgcgcttcat cggattctcc gttgcgcgtg tcgcctgcca tggcggcgac
     1621 ttccccttga cgttattgca cgtgttgcgc actgcgcaac agtgtttcat aattctgcaa
     1681 ggggcagtaa accttgtcaa tccgtaataa caagcgctgc gctaggggaa taccttgggt
     1741 gatctcattc ggaatcaggg agcggcgcgg gcgggcggag aagatccgcc gcgttcgggc
     1801 acgatcaact cggtcgagca tgccatcgaa ttgctgcgct gcctcgccgt ggcgggcggc
     1861 ccgctgggca ccaacgacct cgcccgccgc atcgggctgc acaagagttc ggtctcgcgc
     1921 ctggcggcca cgctggagaa ggcgcgcctg ctgcggcgcg acccggccac cggccgcttc
     1981 ctgctggggc ttgggctgct ggcgctcgcc gcgccgctgt tcgccgattt cgccgtggcg
     2041 gacatcgtcg agcccatcct ggtgaagctc gccgccgaga ccggcgagac ctgcagcttc
     2101 aacatctggg atggacagga cgcggtctcc atcgcccagg ttccgggctc gcattccgtg
     2161 cgcgcctttt ccctgcccgg ccagcgcaag ccggcccatg ccacggcgac gggcaaaatc
     2221 ctgctggcgc atctcgatga ggcggcgctg tcggcttatt gcagcacgcc cttgcgccgc
     2281 tataccgaca ccacgatcac cgattccaag gcgcttgtcg aaaacctcgt ccaggcccgg
     2341 cagcggcgct atgccatcaa tctcggcgag tatgagagcg acgtcggcgg cattgccgcg
     2401 gcggtgttcg acatgtccgg acgcctgttc ggctgcgtcg ccaccaccat gccgctctac
     2461 cgcttcgcgc ccgagcggca cgcggcgctg ggcgcggcgg tgagcgcctg cgccaccgag
     2521 atcgcggagc acctcggcat ttatcgccgc aagggtcggg agggaccacc atgattgctt
     2581 gcaccaggct atcgatcgac ggcgcgctct ggtcctatgc cgagcgcctc tatgcctgcc
     2641 cgggcgtggc cggcgcctgc ctgggcttgc aggatcgcgc gggggtcgac gttgcgctgc
     2701 tcatcgccgc gctctacggc gccggccgcg gctgcgtggt ggacggcgat ttcctgcagc
     2761 ggctcgacgc ggtctcgtcg gctttgcggc gcgaggccgt cgggcccctg cgcgcggccc
     2821 gccgccggct caaggcgcat cccctgaccg cgcggtttgc cgaagtggcc gacttccgcg
     2881 agcggatcaa ggccgtcgag ttgcgcggcg aacaggtcca gcttgccctc atggcgcagg
     2941 tgatcgaaca agcgcttgag ccagcgcttg aaaaaacggc gccggccacg cccgcggcgc
     3001 ccatcgggcg cgacgcttat cggcgggccg gcatggcggt gttgcggcat ttcacgcaag
     3061 gccaggcccc ggagcgcgat gaggatgcgg cggcgatcca ggacatcgcc gatgccgccg
     3121 ccggcgcggc agcccctgat ggccgtgaat tgccaatgcc gtgaatttcg tgctagagtg
     3181 acaacatgaa attcgcgacc cccgcctttt atttttattt ttatggtttt ctgaagccgc
     3241 tggcggagga agggtcgcgt tcacgctgaa acacagcaag aatcccctaa agaaagccgc
     3301 cagcgaacct ggcggctttt ttgttgctgc cagcgccctg gtccaaaacc tggagcaaac
     3361 ccgtgtcgca caataccgat gatcttcgta tccgtgaaat aaaagagctg aatccgcccg
     3421 cgcatgtcat gcgcgagctc gccgccaccc ccgtcgtgtc ggccacggta tatgaggcgc
     3481 ggcgcggcct gcaccgcatc ctgcatggca tggacgaccg cctgatcgtg gtggtcgggc
     3541 cctgttccat ccacgacacc aaagccgcgc tggaatacgc ccgccgcctg aaggccgagc
     3601 acgatcgcct ggccgcagac ctcgagatcg tgatgcgcgt gtacttcgag aagccgcgca
     3661 ccacggtggg ctggaagggc ctgatcaacg accccgacct ggacggcagc ttcaacatca
     3721 acaagggcgt gcgcgtggcg cgcgcgctgc tgctggagat caacagcctg ggcatgtcgg
     3781 ccggctgcga atttctcgac atgatcacgc cgcaatacat cgccgacctg gtgtcgtggg
     3841 gcgcgatcgg cgcgcgcacc accgagagcc aggtgcaccg cgagctggcc tcggggctgt
     3901 cgtgcccggt gggattcaag aacggcaccg acggcaatgt gaggatcgcg gtcgacgcca
     3961 tcaaggccgc ttcgcagccg catcacttct tgtcggtgac caagggcggg cattcggcca
     4021 tcgtgtcgac cacgggcaat gaagactgcc atgtgatcct gcgcggcggc aaggcgccca
     4081 actacgatgc ggccagcgtc gacgcggcct gccaggaaat ggccaagtcg ggcctggcgc
     4141 agcgcatcat gatcgatgcc agccatgcca acagcagcaa gaatcccgag aaccagccgc
     4201 tggtgatgga agacgtggcg cagcagatcg agcgcggcga ggcccgcatc gccggcgtga
     4261 tgatcgaaag ccacctggtg gccggccggc aggagcgggt gcccggccag ccgctggtct
     4321 acggccagag cattaccgat ggctgcat
//
LOCUS       DASWPO010000042        31791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_989900, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000042 DASWPO010000000
VERSION     DASWPO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 31791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31791
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989900"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VGC69_02405"
     CDS             complement(<1..326)
                     /locus_tag="VGC69_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294169.1"
                     /translation="MALVVSGAFLMEQMDSTVISTSLPQMARSLGVEPLRMSVAITAY
                     LISLVIFIPASGVLADRHGARKVFCWALVLFMASSVFCGLSSTLPQLVAGRIVQGASG
                     AMMVPVG"
     gene            complement(552..628)
                     /locus_tag="VGC69_02410"
     tRNA            complement(552..628)
                     /locus_tag="VGC69_02410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(592..594),aa:Pro,seq:cgg)
     gene            814..1509
                     /locus_tag="VGC69_02415"
     CDS             814..1509
                     /locus_tag="VGC69_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HEY0294170.1"
                     /translation="MSHIHFIGGEKGGVGKSLAARMLAQYFIDRAVPFVGFDTDRSHG
                     ALLRFYADYASPIVLDRHDSLDPVMEAALADPQRRILIDLAAQTQQPLTKWLDDSDVL
                     GFAEEHGLALTWWHVMDTGRDSVDLLRRWLDTFGGRIKLVLVLNEVRGDQFEILASSG
                     ERERAETLGASVMTLRRLPDTTMQKIDRQSASFWAVVNHPDRTATGLGLLERQRVKVW
                     LHRAYAELDALDL"
     gene            complement(1573..2538)
                     /locus_tag="VGC69_02420"
     CDS             complement(1573..2538)
                     /locus_tag="VGC69_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0294171.1"
                     /translation="MNLPEIIAPIADDMKAVDAVIRARLNSDVVLIRTIGDYIIGAGG
                     KRMRPAMVLMVARALGYQGTHHQLLAAVLEFIHTSTLLHDDVVDESDLRRGRGTANAV
                     FGNAASVLVGDYLYSRSFEMMVEADNMRIMQILSEATTVIAEGEVLQLLNVHDPEVSQ
                     ERYLQVVRYKTAKLFEAAAQVGAVLAEGTPVQQEAAVAYGRHVGTAFQLVDDVLDYSG
                     DAQALGKNVGDDLREGKPTLPLIRVMEVGTPAQQTLVREAIRTGAADFTAVAAAIQGT
                     DALEHTLAAARAEAELARQALSAWPVSVYRESLLEFCAFAVDRDR"
     gene            2764..3075
                     /gene="rplU"
                     /locus_tag="VGC69_02425"
     CDS             2764..3075
                     /gene="rplU"
                     /locus_tag="VGC69_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY0294172.1"
                     /translation="MYAVVKTGGKQYRVAAGEKLKVEQIPADIGQEITLDQVLSVGEG
                     DQLKVGTPLVAGAVVKATVLAHGRHDKVKIFKMRRRKHYQKHQGHRQNYTEIRIEAIT
                     A"
     gene            3109..3351
                     /gene="rpmA"
                     /locus_tag="VGC69_02430"
     CDS             3109..3351
                     /gene="rpmA"
                     /locus_tag="VGC69_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY0294173.1"
                     /translation="MAQKKGGGSTRNGRDSESKRLGVKVFGGQEILAGGIIIRQRGTR
                     VHPGVNVGLGKDHTLYALIDGKVKFGVKGALNKSIV"
     gene            3604..4740
                     /gene="obgE"
                     /locus_tag="VGC69_02435"
     CDS             3604..4740
                     /gene="obgE"
                     /locus_tag="VGC69_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0294174.1"
                     /translation="MKFVDEATIEVIAGKGGNGVASFRREKFIPKGGPDGGDGGRGGS
                     IFAVADRNINTLVDFRFSRLHRAKNGENGRGSDMYGAAAPDITLRVPVGTIVHDADTG
                     EVLFDLARHHQQVTLAAGGQGGMGNLHFKSSINRAPRQWTPGKEGEQRRLRLELKVLA
                     DVGLLGLPNAGKSTLITRISNARPKIADYPFTTLHPNLGVVRTSPERSFVVADIPGLI
                     EGASEGAGLGHLFLRHLARTRVLLHLVDVASPDPDFDAVGAAIEGARAIVEELRRYDA
                     GLAAKPRWLVLNKLDMVADPQAVRERFCAEFGWNGPVYGISALDGSGTQDLIWALQDY
                     LDAERHKDQDAQDMADGTYVFEDPRFDTTRNDADAPPPPPGGDE"
     gene            4811..5947
                     /gene="proB"
                     /locus_tag="VGC69_02440"
     CDS             4811..5947
                     /gene="proB"
                     /locus_tag="VGC69_02440"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY0294175.1"
                     /translation="MTDSNTAVSVISSSHRLVAKVGSSLVTNEGRGLDHNAVAHWAAQ
                     IASLRQQGRQVVLVSSGAIAEGMARLGWTRRPTAMHELQAAAAVGQMGLCQAYEAAFA
                     GHGLRTAQVLLTHEDLADRSRYLNARSTLFALLRLGVVPIVNENDTVVTDEIKLGDND
                     TLGALVTNLIEADALIILTDQRGLYSADPRKHPGAQFISHAQAGTPELEAMAGGAGSG
                     IGTGGMLTKVLAAKRAAHSGAHTVIASGRERNVLIRLAQGECIGTELQADMPVWSARK
                     QWMADHLHLRGQVVLDAGALRALLREGKSLLPIGVTEARGDFERGDVVACVDEAGREY
                     ARGLVNYSSADTRRILRQPSSKIAQILGYMTDPELMHRDNLVVL"
     gene            complement(5999..6571)
                     /locus_tag="VGC69_02445"
     CDS             complement(5999..6571)
                     /locus_tag="VGC69_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HEY0294176.1"
                     /translation="MHLNSRSFAPMLGAVMLVLAGCQTAPKAADAPVAPPAQAAQEAA
                     PAAPDVQAPAPAASHVAFFIGQSHSEPSLTALKMADGATLYVQRVPILTREDISQAHD
                     LVDKQGRHFVGLRFTEAGTRKLAEVSSESVGKRLVLILDGKVAAAPIIHQPLNRGVLA
                     FSVPTAQGAANLAARIRGEQPAPVKPGPRR"
     regulatory      6698..6806
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6910..8823
                     /gene="thiC"
                     /locus_tag="VGC69_02450"
     CDS             6910..8823
                     /gene="thiC"
                     /locus_tag="VGC69_02450"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY0294177.1"
                     /translation="MNANPKFLAATAEVDAAAIAPLPQSRKIYETGSRPDIRVPFREI
                     SQADTPTMFGGEQNPPLTVYDTSGPYSDPSVKIDIRRGLPELRRAWIEERNDTELLPG
                     PTSDYGKERLADAKLTAMRFDLQRPPRRAKPGANVSQMHYARRGLVTPEMEFVAIREN
                     LRREQYIESLRALGPEGEKLVKRLLRQHPGQSFGASLPGAITPEFVRDEIARGRAIIP
                     ANINHPEIEPMAIGRNFLVKINANIGNSAVSSGISEEVEKMTWAIRWGGDTVMDLSTG
                     KHIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEELTWEIFRDTLIEQAEQGVDYFT
                     IHAGVRLPFIPMTADRMTGIVSRGGSIMAKWCLAHHKESFLYERFEDICEIMKAYDVS
                     FSLGDGLRPGSSYDANDEAQFAELKTLGELTQLAWKHDVQVMIEGPGYVPMQMIKENM
                     ELQLKHCDEAPFYTLGPLTTDIAPGYDHITSGIGAALIGWYGTAMLCYVTPKEHLGLP
                     NKKDVKDGIITYKIAAHAADLAKGHPGAAIRDNALSKARFEFRWDDQFNLGLDPDTAK
                     DFHDETLPKDSMKVAHFCSMCGPHFCSMKITQDVRDYAATLGVNEKDALSKGMEEKAV
                     EFVKKGAEIYHQA"
     gene            complement(8923..9675)
                     /gene="moeB"
                     /locus_tag="VGC69_02455"
     CDS             complement(8923..9675)
                     /gene="moeB"
                     /locus_tag="VGC69_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY0294178.1"
                     /translation="MNDQQLLRYARHILLDELGIEGQEKLLAAQVLVVGAGGLGSPAA
                     LYLATAGVGRITLADDDQVELSNLQRQILHTTASLGRPKAESGRDMLVALNPETRVDA
                     RVARLAGAALDEAVAAADIVLDCTDNFATRHAINRACVKHRKPLVSGAAIRFDGQVSV
                     YDLRDDEAPCYHCLFPEADDTEELSCATTGVFAPLVGIIGSMQAAEAMKVLMGIGEPL
                     AGRLLQLDALTMQWHTVKFRKDPGCPVCGHRG"
     gene            complement(9716..11158)
                     /locus_tag="VGC69_02460"
     CDS             complement(9716..11158)
                     /locus_tag="VGC69_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0294179.1"
                     /translation="MGAVAGVLLSAGVSAIAQRAGAPLPLDELRQLSSVFAAIKDNYV
                     EPVDDKTLINNAISGMVSNLDPHSAYLDADAFKDMQTTTQGEFGGLGIEVGAEDGFVK
                     VISPIEDTPAARAGVLAGDLITKIDDQFTKGMALNDAVKLMRGAPKSPITLTIMRADQ
                     PQPIVIKMQRDIIKVRSVRSKMLDGGVAYVRISQFQERTGSDLVKNLKDLGAKGAPKG
                     LVLDLRNDPGGLLTSAIGSAGAFLPPDSLVVSTDGRVADARHKYLAVPSEYARGEGNY
                     LSDVPAWAKTVPMVVLINVGSASASEIVAGALQDYKRAKLMGNRSFGKGSVQVILPLS
                     QDTAIKLTTSRYFTPKGRSIQATGIVPEYVVADTAQGDLFRLPREADLQHHLANPLGG
                     GEVKGDESTADQPAAKIFEFGGKDDFQLQQAMNLLAGKPIDKGALTTAKADVNAANAK
                     PGDVKPGDVKTDGAQRLTITPSGVEPAPAK"
     gene            complement(11286..12935)
                     /locus_tag="VGC69_02465"
     CDS             complement(11286..12935)
                     /locus_tag="VGC69_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0294180.1"
                     /translation="MRWMAGSLWVAALALAAASAGAATTSDLAGRQTEAEQEQAALRE
                     RINALQKAIDTREASRKDAADALRASETAISRINLQLRDLGEAQRRAESELAGIEKQI
                     TAQQAVLGQRREELARQLRAQYSSGLSPWATLLSGDDPQQLGRNLGYLDFVSQARARA
                     LAALRADLDRMAQLQSAAAARRADLARVADETTTQKNNLLAEQKERAAVVARIEGQLA
                     AQRAEAGKLGRDDQRLSHLIDDLQVAVARQAAEEARRAEAARKAEEARKAEEARKAEE
                     ARKAEAARKVADAARKADEARQARQAADQARRSAAANEAAQAREQAERAAAAARPGQD
                     GSVALADPDAAGLRPRLTESATPRLTPPELQQPTPAPVPAPAPVSPPPPSPAPSADKP
                     DSRPAAVAQAEEPDDEDDAPKRRPHVTGTGLKHGLPLPVAGGTPQGRFGVGRPDGGVW
                     RGIVLRAPEGTPVKAVAAGVVVYADWLRGFGNLIIIDHGQQYLTVYAYNQSLLKRVGD
                     PVAAGDTIASVGATGGQVESGLYFEIRYRGTPVDPAQWLAL"
     gene            complement(12978..13727)
                     /gene="gpmA"
                     /locus_tag="VGC69_02470"
     CDS             complement(12978..13727)
                     /gene="gpmA"
                     /locus_tag="VGC69_02470"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY0294181.1"
                     /translation="MHKLVLMRHGESQWNLENRFTGWTDVDLTDLGHEQARQAGLLLK
                     QEGYTFDIAFTSVLKRAIRTLWIALDAMDAMYTPVGNSWRLNERHYGALQGLNKAETA
                     AKFGDEQVLVWRRAYAIAPDALGEDDPRHPRFDPRYARVPAGELPATECLKDTVARVL
                     PFWNDSIAPAIRAGRKVLISAHGNSLRALIKYLDNMSEDDIVGLNIPTGQPLVYELDD
                     DLKPIRHYYLGDPAAIEAAMAAVAAQGKAKK"
     gene            13892..14314
                     /locus_tag="VGC69_02475"
     CDS             13892..14314
                     /locus_tag="VGC69_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0294182.1"
                     /translation="MDLLHFLLDKSNIIIVAVAVVSGVMLALPNLRRSRGGAALSTNE
                     AIQMSNQRQAVWVDVRPAEQFQAGHIAQARSLPAADVEQKTASLPKNKPLILVCEQGK
                     DAPRIAARLRAQGYADATALEGGMRAWFTAGLPVTQKS"
     gene            14342..14599
                     /gene="grxC"
                     /locus_tag="VGC69_02480"
     CDS             14342..14599
                     /gene="grxC"
                     /locus_tag="VGC69_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY0294183.1"
                     /translation="MQKVVMYSKDYCPYCARAEALLMERGVTDFEKIRIDHEPGQREA
                     MIERTGRRTVPQIYIGATHVGGCDDLVALDRQGGLLPLLNG"
     gene            14651..15157
                     /gene="secB"
                     /locus_tag="VGC69_02485"
     CDS             14651..15157
                     /gene="secB"
                     /locus_tag="VGC69_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY0294184.1"
                     /translation="MADQNTQQESGAPAFNMQRVYLKDLSLEMPNAPHIFLEQETPQV
                     EVSVNVGGQRLADTIFESTVTATVTTRIGDKVLYLVEATQAAIFELANIPPEQMDPIL
                     GIVCPTMLYPYLRASVADAITRTSMPPLHLAEVNFQALYEQQMAQQPGQSNGNDSGII
                     LPPGATRQ"
     gene            15184..16242
                     /locus_tag="VGC69_02490"
     CDS             15184..16242
                     /locus_tag="VGC69_02490"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY0294185.1"
                     /translation="MNTPTALRVVVLGAGSWGTALAAAAARRHPTLLWARNAAQAQAM
                     ASEHENSRYLPGVALPESLAIESDLAAALAFLQGGPAQPANPGLIILGVPVAGLEEAC
                     RALARHLSAMDPAGAAAHVPVVWTCKGFEETTARLPHEIAQAALPAGWRGGVLSGPSF
                     AREVAQGLPVALTIASQEPAVRDTAIAALHGGPMRVYASTDVVGVEVGGALKNVIAVA
                     CGIADGLRLGTNTRAALITRGLAEISRFGQALGAQAETFAGLTGLGDLVLTATGDLSR
                     NRRVGLEIGAGRTLADILASGITAEGVRCARAVLARARQMNVELPITEAVCAVLFDGV
                     APATAVSALLAREVRYES"
     gene            16405..16932
                     /locus_tag="VGC69_02495"
     CDS             16405..16932
                     /locus_tag="VGC69_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294186.1"
                     /translation="MKNSTLIKTAALALIVSLGAACGTAEAWGRYGGHGGYGGHGGYY
                     HRGPNGWFIGTALGLAAAGTVIALSQPQYVYPAPVYAAPVYPAAPVYAAPPVVQYAPQ
                     PQQAMASTDVIAYPTRGQNPQQQANDRSACEQWAANQSGYDPAQASRWTTTVQTDSYS
                     RSIGACMKGRGYSIN"
     gene            complement(16999..17469)
                     /locus_tag="VGC69_02500"
     CDS             complement(16999..17469)
                     /locus_tag="VGC69_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY0294187.1"
                     /translation="MFHIVLVCPEIPPNTGNAIRLCANSGAQLHLVRPLGFDLDDARL
                     RRAGLDYHEWQPVQVHDSLQQALQATGAVARRIYAMTTRATHHVGEAVFQPGDVFVFG
                     RETAGLSAEHLALFDQAQRLRLPMRPGQRSLNLSNAVAVTVFEAWRQQGYAGGV"
     gene            complement(17482..18297)
                     /locus_tag="VGC69_02505"
     CDS             complement(17482..18297)
                     /locus_tag="VGC69_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020699687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0294188.1"
                     /translation="MTVTRIIPHPRAGALPALWRGLERRWRSLLAGIGSDCPACEGAA
                     RGGLLCQPCAQAVLEQAAARRCPRCAVYLSAGQAGRHGGLCVECALYPPAFVRAVAAF
                     DYADPFDRLIARYKGARRHVVAGALAQVLAQAVRTAEAARAPGPRTGRLPIDAVVPVP
                     ASRASLRQRGFNPAGELARALSRRLELRLLRGVLRRCRETGVRQASLARSEREAAALD
                     AYLCTRRLDGLRIALVDDVMTTGSTADAAARALLAAGAASVSVWVAARTPRPL"
     gene            18398..19324
                     /locus_tag="VGC69_02510"
     CDS             18398..19324
                     /locus_tag="VGC69_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0294189.1"
                     /translation="MSLPASPLPTLPLSTAHVQRQFARRGDLAEAQFLYGEVARRMLG
                     RLSYIRLDPKQMLDAGCGAGDNLALLRERYPQAAYIGLDHCEPLLAHTRRRFMAAGMG
                     AWLGRLVGNKQPALQYVRADLADTGLAPESLDLVWSNLALHWHPEPHAALAEWRRVLR
                     VGGLAMFSCLGPGTLRELRQALADAGLRTATPGFVDMHDFGDLLLENGYADPVMDQET
                     LTLTYETPQKLLADVWALGGNPATGRRAGLAGRAWRERLCEALAAQRGQDGRIALSIE
                     VAYGHAWRAAARRAATGETRVSLEAIGGRRAK"
     gene            complement(19349..20539)
                     /locus_tag="VGC69_02515"
     CDS             complement(19349..20539)
                     /locus_tag="VGC69_02515"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEY0294190.1"
                     /translation="MESLPFASSAPNTLGIELELQLIDPTSFDLTAASDDLLAQLAGH
                     CMADRVKPEITRSMIELNSSVHEHPAGLLAEMREMRDVVGAAADALGIAVSGGGAHPF
                     MRWQDRCIHDSPRFHYIAETYGYLARQFTVFGQHIHLGTTSGDAAVRLVRGLSPYVPH
                     FIALSAASPYFEGVDTLYSCCRLNAVNNFPLSGHMPADVRDWYGFEAHIAQLRATGLA
                     ENIKDLYWDIRPKPEFGTVEIRVCDTPLTVERACQLAAFAQALAVLVSRTEEPDETAW
                     LAYRSNHFQACRFGLHGAYVTPQGERVRLGDHLRLLLQQLVPVAEELGTVDMLQALRD
                     DLQRGGNDARWLRAEFHRLRELPGVVESMTRLWRGGGAASGALVDSAYGRRRVRAHSE
                     PMLH"
     gene            complement(20758..21402)
                     /locus_tag="VGC69_02520"
     CDS             complement(20758..21402)
                     /locus_tag="VGC69_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0294191.1"
                     /translation="MRAGTVQASGMAGCMGILLAGGYGRRYAQAAGGGDKLLASLPDG
                     RPVAQASAAAMLAVLPGVWAVVRPESQALAGLLAGMGCRVLRTGRAREGMGGSLAAAA
                     EALLAQAGAAAALAPTHCLVALADMPWVAPASHVRVAESGCGHPIVAAACQGRRGHPV
                     SFAAALWPELAALAGDEGARGLLTRHGVVLAETGDPGVLADIDQPADLMGPARP"
     gene            complement(21404..21601)
                     /locus_tag="VGC69_02525"
     CDS             complement(21404..21601)
                     /locus_tag="VGC69_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294192.1"
                     /translation="MKIPILGAGRLGTRVAANLVPVQNDIAAIDTEPAPPGYLQERNI
                     MIGRLALPTVPRLFTPLFWRR"
     gene            complement(21598..22281)
                     /locus_tag="VGC69_02530"
     CDS             complement(21598..22281)
                     /locus_tag="VGC69_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0294193.1"
                     /translation="MARILVVDDEVGIRELLSEILYDEGHTVELAENAAEARAARLRM
                     RPDLVLLDIWMPDTDGVSLLKEWGSQGLLDMPVIMMSGHATIDTAVEATRIGAMDFLE
                     KPITLQRLLKTISSGLSRGRAPYPASAAPALAPVVVPLEDELDPPPAAPVAEAQAQTG
                     NGQLGNISLDLPLREARDAFERIYFEYHLVRENHSMTRVSERTGLERTHLYRKLKQLG
                     IESVRKRST"
     gene            complement(22334..24574)
                     /locus_tag="VGC69_02535"
     CDS             complement(22334..24574)
                     /locus_tag="VGC69_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0294194.1"
                     /translation="MTRFLRFVLIIGGLSGLGLLGLLAWSTGNASRFARYYDVLLLLN
                     GIFALALLAWVVGLTGRLVKQIRRRQFGARLTARFSLAFALIGVVPGALIYTLSVQFL
                     SRSIESWFNVRVDSALEAGLNLGRAVLDTQLADLNSRARAMASELNNLNDNDVSLALT
                     RLRENNGVQEALVFTSSGRMVAFSTSQYGQLLPALPPSTVMNQLKLAGGYSAAEASEP
                     AVRGAESTLLLRVVVPLVTPVRFDSGLGTASEPRWMQLVQPVPDQLAHNANRVQQGFG
                     DYQELALSRLGLRKLYGITLTLALLLAMLASIAVALSLSKRLVRPLLRLAAGTQAVSV
                     GDYRPLPEPPERDEVGQLTRSFNAMTRQLDEARRMVESNRRQLERSNIYLESVLANLS
                     SGVLAFDEGFRVTTVNQGAQTILQADLRSVIGRPLETADGMIEFANIVRQAFSEHAAV
                     GSERTHWQQQFEIKRGTQGEAVTLLARGTHLHVDGRGNGYLVVFDDITEVISASRTVA
                     WGEVARRLAHEIKNPLTPIQLSAERLAMKLAGRLEPADAQMLTRATNTIVNQVGSLKK
                     MVDDFREYARTPPAVMQRIVFNNLVADVLALYGWEAGGGEGPRALNLDVRLESGLPDI
                     EGDPTQLRQVIHNLLSNARDAVTDLGEAGLVRVSTQLVQSARSDGTSERAVRFTVSDN
                     GPGFAAQVAQRAFEPYVTTKAHGTGLGLAIVRKIIDEHDGRIDLANRKEGGARVSILL
                     TRLAAE"
     gene            complement(24591..25193)
                     /locus_tag="VGC69_02540"
     CDS             complement(24591..25193)
                     /locus_tag="VGC69_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEY0294195.1"
                     /translation="MTPRACPSLTISRGFWAFLLAIFLFGVSAGQVAQAADPRIERID
                     PSVHEGRLEIDADVRFDLNDQLRNAAQRGVPLFFTADLVITRSRWWWFDHTVVNTSMT
                     WRIVYNALTRQWRAGAGELSLPVASLDEAMDVVRHIRNWQVSTTEKFDSDTQYAGQLR
                     VRLDTSMLTRPFQVNALNSTSWSPATPWTDFSFALARPEK"
     gene            complement(25213..26526)
                     /gene="rsmB"
                     /locus_tag="VGC69_02545"
     CDS             complement(25213..26526)
                     /gene="rsmB"
                     /locus_tag="VGC69_02545"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY0294196.1"
                     /translation="MPQNLTPPLSQLLLAAADAVQAVLQGRSLTEALAQVDAALRPGT
                     QALGFHAMRQLGWADEIAGELLEKSPGARLGALLRVALTLLKPAAEPLPGMPSYAPHT
                     VVNQAVQAADGERKLAPYKGLLNACLRRFLREQEALERAVADRPQARWNHPPWWVDRL
                     KADYPDRYAELLEAANVPAPLTLRVNRRRAAREQVLAAFQAEGIAAEPAGEAGLVLAA
                     PRPVAQLPGFAQGWWSVQDAGAQLAAPLLAPRDGMRVLDACAAPGGKTAHLLELADLE
                     LLALDVDAGRLGRVGQNLDRLGLRTPRVALKQADAADPDAWWDGRPFDAVLADVPCTA
                     SGIVRRHPDIRWLRRADDVARTAGLQARIADALWRTVAPGGRLLYVTCSIFPAEGEVQ
                     ARAFQARHAEARRLPAPGQLLPVAIGATPAARHDGFFYALFAKQG"
     gene            26623..27951
                     /gene="rimO"
                     /locus_tag="VGC69_02550"
     CDS             26623..27951
                     /gene="rimO"
                     /locus_tag="VGC69_02550"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY0294197.1"
                     /translation="MSTVTAPKVGFVSLGCPKALVDSERILTQLRTEGYQITPSYDDA
                     DVVVVNTCGFIDSAKAESLEAIGEAIAENGKVIVTGCMGVEESVIRGVHPSVLAVTGP
                     QQYEQVVRAVHDAAPPRADHDPFVDLVPPQGVKLTPRHYAYLKISEGCNHRCSFCIIP
                     SMRGDLVSRPVGDVLNEAERLVRAGVKELLVISQDTSAYGVDLKYRSGFWNGRPVKTR
                     MTELCAALSELGVWTRLHYVYPYPHVDEVIPLMAEGKILPYLDIPFQHASPRVLKAMK
                     RPAFEDKTMARIKQWRAACPDLTIRSTFIVGFPGETEEDFQYLLDWMTEAQLDRVGCF
                     QYSPVEGAPANALGGHVPDELKQERWERFMAHQQAISAARLSAKVGREIDVLIDGPDE
                     DGDIVGRSSADAPEIDGLVYVDSAKALKAGDMVRVRVTDADEYDLWGETL"
     gene            27951..28448
                     /locus_tag="VGC69_02555"
     CDS             27951..28448
                     /locus_tag="VGC69_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0294198.1"
                     /translation="MPDRLHARARQLIAQLGLQPHPEGGHYREIFRSGLQVRRQPDGL
                     ARSALTSIFFLLPRGACSRWHRVAQADEAWHHYEGDPIALHVLLPGARQAETWLLGPL
                     GAGHPDALPARIVPANAWQAAQPLGDYALVGCSVGPGFEFADFAMARDLPAAERPAAL
                     DAAFL"
     gene            28496..29083
                     /locus_tag="VGC69_02560"
     CDS             28496..29083
                     /locus_tag="VGC69_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY0294199.1"
                     /translation="MALENICVYCGSNKGRDPLYIERAQAFGRELARRGLGLVYGGAD
                     VGIMGAVADTVLAAGGRVIGVIPESLRTKEMAHQGLTELHVVPTMHERKQLMAEKADG
                     FVALPGGAGTLDEIFEVWTWAQLGFHRKPCGLFNVAGYFDQLAAFLDHAAGEGFMRAE
                     HRAMLMVESDPAALLDRYAAYHAPTVSKWIATGGR"
     gene            29086..29658
                     /locus_tag="VGC69_02565"
     CDS             29086..29658
                     /locus_tag="VGC69_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294200.1"
                     /translation="MQDQIPHDTAAVLRDYFHAKDENRPWYMARAFAPDAVLKMALRT
                     QAIAFPPESRGVQAIADTLSRRFGQTYDNVYTFYLSRPADGAVLDEYSCDWIVAMTEK
                     ATGQARVGCGRYDWTFRQSPHQAERLVITIETMLTLPASQADAVLDWITRLPYPWTDA
                     QRIVATAPAIEALAPVLQWIRRDAPHTLTA"
     gene            29691..30317
                     /locus_tag="VGC69_02570"
     CDS             29691..30317
                     /locus_tag="VGC69_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0294201.1"
                     /translation="MPTQDPQRITDLDALTALYGEPSTASLVKEMDHVHPHYRAFIET
                     APFAALATCSPGGLDVSPRGDPAGFVHIEDEKTLMIPDRRGNNRVDSLRNILADPRVA
                     LLFLVPGVGETLRVNGRAEIRVEPALLERFAMDGKPPRSVLVIHVETVFFQCSRAVVR
                     AGLWDAGRQIARGRLPSTGSMLSDFSKGTFDGAAYDRDLDARVQSTLY"
     gene            complement(30369..31301)
                     /gene="fmt"
                     /locus_tag="VGC69_02575"
     CDS             complement(30369..31301)
                     /gene="fmt"
                     /locus_tag="VGC69_02575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY0294202.1"
                     /translation="MRLVFAGTPDFSRIALQALLAAGHDIPLVLTQPDRPAGRGLKLT
                     PSPVKQAALEAGIEVAQPRSLRLDGKYPDEAGAARDLLERIAPDVMVVAAYGLILPAW
                     TLRLPRLGCLNIHASLLPRWRGAAPIQRAIEAGDAETGVTIMQMDEGLDTGDMLLERV
                     VPIGAQQTAAELHDALADAGGRAIVEALAALPALAPRRQPEAGATYAAKLDKAEAALD
                     CTQPAPLLARRVRAFNPVPGATLRLPGLAEPVKVWKAQALPGGAGAPGSVLDATERGI
                     DIATGEGVLRLLELQKAGGKRQPVDVFVRGWQPA"
     gene            complement(31338..>31791)
                     /locus_tag="VGC69_02580"
     CDS             complement(31338..>31791)
                     /locus_tag="VGC69_02580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0294203.1"
                     /translation="AGKRALLAVSVGGPAKDYSPRGINGPLEQLLFPITHGTLFFPGM
                     QVLPTFAVYGTGGIDAQGMAAAAAAWRGRVERLFDDEPIAFRPQNGGDYPDRHALADH
                     IAPHQAGLTVHIAAVSRPAAETGESRSSGPAAFPERRGEDGRDPRAPR"
BASE COUNT         5058 a        11161 c        10828 g         4744 t
ORIGIN      
        1 cccaccggca ccatcatggc cccgctggcg ccctgcacga tgcggccggc gaccagttgc
       61 ggcagcgtcg acgacaagcc gcagaacacc gaactggcca tgaacaggac gagcgcccag
      121 cagaacacct tgcgcgcgcc atgccggtcg gccagcacgc cgctggccgg gatgaagatc
      181 accaggctga tcagataggc ggtgatggcc acgctcatgc gcagcggctc gacgcccagg
      241 ctgcgcgcca tctgcggcag cgacgtcgag atcaccgtcg agtccatctg ctccatgagg
      301 aaggcgcccg atacgacgag cgccacggga agatagctac ggggggagga cgctgcggaa
      361 ggggaggccg gcatgatcgg aaaacgcgac tgaacgggag attatagaaa cttatcccac
      421 gcgttccgcg cataagcata tgcccagcgg tgcgagaaca gccctcgagc agaggacgct
      481 tgccttgact tgataaaacg atgctcttgc gaagtgccta aaccggaaac cgacccgctt
      541 cgcaaggaag atggtcggag cgagaggatt cgaacctccg actcctgcgt cccgaacgca
      601 gtactctacc aggctgagct acgctccggt tggctgacgc gtcaaaagca aagacgtgag
      661 attctacctg aaatttccag ccgacaaaaa ccgccgcccc tccggcacgc catggaataa
      721 acatttgaca ccgcggccgg tctccccgca tgcttgcgca gccatttcct ttcggcgcga
      781 cccatccctg agccacgaca cgagacccta ggcatgagcc acatccactt catcggcgga
      841 gaaaaaggcg gcgtcggcaa atcgctggcc gcccgcatgc tggcgcagta tttcatcgat
      901 cgagccgtgc ccttcgtggg cttcgatacc gacaggtcgc acggcgcgct gctgcggttc
      961 tatgccgact atgcctcgcc catcgtgctc gaccggcacg acagcctcga tcccgtcatg
     1021 gaggcggccc tcgccgaccc gcagcgccgc attctgatcg atctcgcagc ccagacgcag
     1081 cagccgctga ccaagtggct cgacgattcg gacgtcctgg gttttgccga agaacacggc
     1141 ctcgcgctga cgtggtggca cgtcatggac acgggccgcg attcggtcga cctgctgcgg
     1201 cgctggctcg acacgttcgg cgggcggatc aagctggtgc tggtgctcaa tgaagtccgc
     1261 ggcgatcagt tcgagattct ggcatcatcc ggcgaacgcg aacgggccga aacgctgggc
     1321 gccagcgtga tgacgctgcg ccggctgccc gatacgacca tgcagaagat cgaccggcaa
     1381 agcgccagct tctgggcggt ggtcaatcat ccggaccgca ccgccacggg gctcggcctg
     1441 ctggagcggc agcgcgtcaa ggtatggctg caccgcgcct acgcggagct ggacgcgctc
     1501 gacctgtaag cccccgctcc gcacggccgg ggaatcagcg gcggggccgc gcccggctgc
     1561 cgtgatcggc gatcagcgat cgcggtcgac cgcgaaagcg caaaattcca gcagggactc
     1621 gcggtaaacg gaaaccggcc aggccgacag ggcctggcgg gccagttcgg cttcggcgcg
     1681 ggccgcggcc agggtgtgtt cgagcgcgtc ggtgccctgg atggcggcgg ccaccgcggt
     1741 gaagtcggcg gcgccggtgc ggatggcctc gcgcaccagg gtctgctgcg ccggggtgcc
     1801 cacttccatg acgcggatga gcggcagcgt gggcttgcct tcgcgcaggt cgtcgccgac
     1861 gttcttgccc agggcctgcg cgtcgccgct gtagtcgagc acgtcgtcga ccagctggaa
     1921 ggcggtgccc acgtggcggc cgtaggcaac ggcggcctcc tgctggacgg gtgtgccctc
     1981 ggccagcacg gcgcccacct gggcggcggc ctcgaagagc ttggccgtct tgtagcgcac
     2041 cacctgcagg tagcgctcct gcgacacctc ggggtcgtgc acgttgagca gctgcagcac
     2101 ctcgccctcg gcgatcaccg tggtggcctc ggacaggatc tgcatgatgc gcatattgtc
     2161 ggcctcgacc atcatctcga acgagcgcga atacagatag tcgcccacca gcacgctggc
     2221 ggcattgccg aacaccgcat tggccgtgcc gcgcccgcgg cgcaggtcgg attcgtcgac
     2281 cacgtcgtcg tgcagcagcg tggaggtatg gatgaactcc agcaccgcgg ccaggagctg
     2341 atggtgcgtg ccctggtagc ccagcgcccg ggccaccatc agcaccatgg ccggccgcat
     2401 gcgcttgccc cccgccccga tgatgtagtc gccgatcgta cggatcagga ccacgtcgga
     2461 attgagccgg gcgcggatga ccgcgtcgac ggccttcatg tcgtcggcta tgggagcaat
     2521 gatctcgggg aggttcaagt cggttccggg tatgaagcat gccgcgcgca ggcgcgtgcc
     2581 gggcaggccc gcccgacgat gatgacggcg ggaattcgac gcgattatac ggggtggggg
     2641 tccgtcacct ctaaatcctt ggcctgattg agctttttga cttttgcacg gtgtcaagct
     2701 agaatggcgg gctctggctg tcaaaagccg gaagctcttt tccacattta aggaatttcc
     2761 cccatgtacg cggtcgtaaa aaccggtggc aagcagtatc gcgttgccgc tggcgaaaaa
     2821 ctcaaggtag aacagatacc ggctgacatc gggcaagaaa tcaccctgga ccaggtgctg
     2881 tccgtgggcg aaggcgacca actgaaagtt ggcaccccgc tcgtggccgg cgccgtggtc
     2941 aaggctacgg ttcttgcgca cggtcgccac gacaaggtca agatcttcaa gatgcgccgt
     3001 cgcaagcact atcaaaagca tcaaggccac cgtcagaact ataccgagat ccgcatcgaa
     3061 gccatcacgg cttgatcgga catccggttt tttagcagga gctagaacat ggcacagaaa
     3121 aaaggcggcg gctcgactcg caacggccgc gactcggaat cgaagcgcct gggcgtgaag
     3181 gtgttcggcg gccaggaaat cctggctggc ggcatcatca ttcgccagcg tggcacgcgc
     3241 gtccatcccg gcgtgaacgt cggcctgggc aaggaccaca ccctgtacgc gctgatcgac
     3301 ggcaaggtga agttcggcgt caagggcgcc ctgaacaagt ccatcgtcta ggtcgtcacg
     3361 gaataatccc ggcacgcacc gcacaaagcc ccgccaggcg gggctttgtc gttttaagat
     3421 gcttgctgtg agtctgtttt ggcgccgggc cccgagaggc ctggcggggc gcctctgccg
     3481 cggggcagcc cgcagggccg atggcccgtc gtcttgggcc cgagcccgac gcagcgaagc
     3541 cccgccaggc ctctcggggc ccggcgccaa aacagacgct caagaactca aacaaagacc
     3601 actatgaaat tcgtcgacga agccaccatt gaagtcattg ccggcaaggg cggcaacggc
     3661 gtggcgagct ttcgccgcga aaaattcatc cccaagggcg gtcccgacgg cggcgacggc
     3721 gggcgcggcg gcagcatctt cgccgtggcc gaccgcaaca tcaacaccct ggtcgacttc
     3781 cgcttctcgc gcctgcaccg cgccaagaac ggcgagaacg gccgcggttc cgacatgtac
     3841 ggcgccgccg cccccgacat caccctgcgc gtgcccgtgg gcaccatcgt ccacgacgcc
     3901 gacaccggcg aagtcctgtt cgacctggcc cgccaccacc agcaggtcac cctggccgcc
     3961 ggcggccagg gcgggatggg caacctgcac ttcaagtcca gcatcaaccg cgcgccgcgc
     4021 cagtggaccc ccggcaaaga aggcgaacag cgccgcctgc gcctggagct caaggtgctg
     4081 gccgatgtgg gcctgctggg cctgcccaac gcgggcaaat ccacgctcat cacccgcatc
     4141 tccaacgcgc gccccaagat cgccgactac cccttcacca cgctgcatcc caacctgggc
     4201 gtggtgcgca cctcgcctga gcgcagcttc gtcgtggccg acatccccgg cctgatcgag
     4261 ggcgcgtcag agggcgccgg cctgggccac ctcttcctgc gccacctggc ccgcacccgc
     4321 gtgctgctgc acctggtcga cgtcgccagc cccgacccgg acttcgacgc cgtcggcgcc
     4381 gccatcgaag gcgcgcgcgc catcgtcgag gaactgcgcc gctacgacgc cggcctggcc
     4441 gccaagccgc gctggctggt gctcaacaag ctcgacatgg tggccgaccc gcaggccgtg
     4501 cgcgagcgct tctgcgccga attcggctgg aacggcccgg tatacggcat ctcggccctg
     4561 gacggctcgg gcacgcagga cctgatctgg gccctgcagg actacctcga cgccgagcgc
     4621 cacaaggacc aggacgccca ggacatggcc gacggcacct atgtgttcga agacccgcgc
     4681 ttcgacacca cccgcaacga cgcggacgcc ccgcccccgc cgcccggcgg tgacgaataa
     4741 gccataattt cgcattgggt tcgccggcct cgcggccggc tttcgcgtcg ctgtatttac
     4801 ggtcatcgcc atgactgact cgaacaccgc tgtttccgtc atttcctcgt cccaccgcct
     4861 ggtggccaag gtgggctcgt ccctggtcac caacgaaggc cgcggcctgg accacaacgc
     4921 cgtggcccac tgggccgcgc agatcgcctc gctgcgccag caggggcgcc aggtggtgct
     4981 ggtgtcttcc ggcgccatcg ccgagggcat ggcgcggctg ggctggaccc gccgccccac
     5041 cgccatgcac gagctgcagg cggccgcggc cgtgggccag atgggcttgt gccaggccta
     5101 cgaggcggcc ttcgccggac acggcctgcg caccgcgcag gtgctgctca cccatgaaga
     5161 cctggccgac cgcagccgct acctgaacgc gcgctcgacg ctgttcgcgc tgctgcgcct
     5221 gggcgtggtg cccatcgtca acgagaacga cacggtggtg accgacgaga tcaagctcgg
     5281 cgacaacgac acgctgggcg ccctggtcac caacctgatc gaggccgacg cgctcatcat
     5341 cctgaccgac cagcgaggcc tgtacagcgc cgacccgcgc aagcaccccg gcgcgcagtt
     5401 catctcgcac gcccaggcgg gcacccccga gctcgaagcc atggccggcg gcgccggcag
     5461 cggcatcggc acgggcggca tgctgaccaa ggtgctggcc gccaagcgcg cggcccatag
     5521 cggcgcccat accgtgatcg cttccgggcg cgaacgcaat gtgctgatac gcctggccca
     5581 gggcgaatgc atcggcaccg agctgcaggc cgacatgccg gtgtggtcgg cgcgcaagca
     5641 gtggatggcc gaccacctgc acctgcgcgg ccaggtggtg ctcgacgcgg gcgcgctgcg
     5701 cgcgctgctg cgcgaaggca agagcctgct gcccatcggc gtgaccgagg cgcggggcga
     5761 tttcgagcgc ggcgacgtgg tggcctgcgt cgacgaggca ggccgcgaat atgcccgcgg
     5821 cctggtcaac tattcatcgg ccgacacgcg gcgcatcctg cgccagccat cgtcgaagat
     5881 cgcgcagatc ctgggctaca tgaccgaccc ggaactgatg caccgcgaca acctggtcgt
     5941 gctttgaggc atcggtcctt ggccgggccg ccgcgcagac cgcggcggcc cggcggattt
     6001 agcggcgcgg cccgggcttg accggggccg gctgttcgcc gcggatgcgg gccgccagat
     6061 tggcggcgcc ctgggcggtc ggcacgctga aggccagcac gccgcgattc aggggttggt
     6121 ggatgatcgg cgccgccgcc accttgccat cgagtatcag caccaagcgc ttgcccacgc
     6181 tctcgctgct gacctcggcg agcttgcgcg tgccggcttc ggtaaagcgc agcccgacga
     6241 aatgcctgcc ctgcttgtcg accaggtcat gggcctggct gatatcctcg cgggtcagga
     6301 tgggaacgcg ctgcacatag agggtggcgc cgtcggccat tttcagggcc gtcaggctgg
     6361 gttcggaatg gctctggccg atgaagaagg ccacatggct ggcggcgggg gcaggcgcct
     6421 ggacgtccgg cgcggcgggc gcggcttcct gcgccgcctg cgctggcggc gccaccggcg
     6481 cgtcggcggc cttgggcgcg gtctggcacc cggccagcac cagcatgacg gcgcccagca
     6541 tgggagcgaa cgagcgtgaa ttcagatgca tggcgcgttc cttgtgttgc gatcgatgaa
     6601 gtgtaccggc ccgtcctcgc cgaagtccga ccggggcccc ccagtttgta atgggtcgca
     6661 acccaaaccg gcaagccggg aagcgcttta tacttgcatt tgcacgcagg ggtactcgcc
     6721 gcggggccaa accccgcagc gtgttgagac agtcccttgg caccagtacg gataatgccg
     6781 atgctgggag cgtattccac cagcccgccg catccttgca ccttcgatgc gccccggccg
     6841 gaatccatcc cgactcggct ccagaacgca tcaacgcgcc ccctatgcgc gtcacccttg
     6901 gagctaccca tgaacgccaa tcccaaattc ctggccgcca ccgccgaagt ggatgccgcc
     6961 gccatcgcgc cgctgccgca atcgcgcaaa atctacgaaa ccggctcgcg ccccgacatc
     7021 cgcgtgccct ttcgcgaaat ctcgcaggcc gacacgccca ccatgttcgg cggcgagcag
     7081 aacccgccgc tcacggtgta cgacaccagc ggcccctact ccgacccgtc ggtgaagatc
     7141 gacatccgcc gcggcctgcc cgagctgcgc cgcgcctgga tcgaagagcg caacgacacc
     7201 gaactgctgc cgggccccac cagcgactat ggcaaggaac gcctggccga cgccaagctc
     7261 acggccatgc gcttcgacct gcagcgcccg ccgcgccgcg ccaagccggg cgccaacgtg
     7321 tcgcagatgc actatgcgcg ccgcggcctc gtcacgccgg aaatggagtt cgtcgccatc
     7381 cgcgagaacc tgcgccgcga gcagtacatc gaaagcctgc gcgccctggg ccccgaaggc
     7441 gagaagctgg tcaagcgcct gctgcgccag cacccgggcc agtccttcgg cgcctcgctg
     7501 cccggcgcga tcacgcccga gttcgtgcgc gacgagatcg cccgcggccg cgccatcatc
     7561 ccggccaaca tcaatcaccc cgaaatcgag cccatggcca tcggccgcaa tttcctggtg
     7621 aagatcaacg ccaatatcgg caactcggcc gtgagctcgg gcatttccga ggaagtggaa
     7681 aagatgacct gggccatccg ctggggcggc gacacggtga tggatctctc caccggcaag
     7741 cacatccatg aaacgcgcga gtggatcatc cgcaactcgc ccgtgcccat cggcaccgtg
     7801 cccatctacc aggcgctgga aaaggtcgac ggcaaggccg aggagctcac ctgggaaatc
     7861 ttccgcgaca cgctgatcga gcaggccgag caaggcgtgg actatttcac catccacgcc
     7921 ggcgtgcgcc tgcccttcat ccccatgacg gccgaccgca tgacgggcat cgtctcgcgc
     7981 ggcggttcca tcatggccaa gtggtgcctg gcgcaccaca aggaaagctt cctgtacgag
     8041 cgcttcgaag acatctgcga gatcatgaag gcctacgacg tgagcttctc gctgggcgac
     8101 gggctgcgtc cgggctcgag ctacgacgcc aacgacgagg cgcagttcgc cgagctgaag
     8161 acgctgggcg aactgacgca gcttgcctgg aagcacgatg tgcaggtgat gatcgaaggc
     8221 ccgggctacg tgcccatgca gatgatcaag gaaaacatgg agctccagct caagcactgc
     8281 gacgaagcgc ccttctacac cctgggaccg ctgaccaccg atatcgcgcc gggctacgac
     8341 cacatcacct cgggcatcgg cgcggcgctg atcggctggt acggcacggc gatgctgtgc
     8401 tacgtgacgc ccaaggaaca cctgggcctg cccaacaaga aggacgtgaa ggacggcatc
     8461 atcacgtaca agatcgcggc gcacgcggcc gacctggcca agggccaccc gggcgcggcg
     8521 atccgcgaca acgcgctgtc gaaggcgcgc ttcgagttcc gctgggacga ccagttcaac
     8581 ctggggctgg atccggacac ggccaaggac ttccacgacg agaccttgcc caaggactcg
     8641 atgaaggtgg cgcacttctg ttcgatgtgc gggccgcact tctgcagcat gaagatcacg
     8701 caggacgtgc gcgactatgc cgcgacgctg ggcgtgaacg agaaggacgc gctgtccaag
     8761 ggcatggaag agaaggcggt ggagttcgtg aagaagggcg cggagatcta tcaccaggcc
     8821 tgagggccgc actggcggga tccgtgctca acgcccgtca tgcctggcat tggcgggcgt
     8881 tttgtttttg ggttccaaaa ccgtctggcg tgcgatgcca ggtcaacccc gatgcccgca
     8941 aaccgggcac ccggggtcct tccggaactt gacggtgtgc cactgcatcg tcagcgcatc
     9001 gagctgcagc aaacgacccg ccagcggctc gcctatcccc atcagcacct tcatcgcctc
     9061 ggccgcctgc atgctgccga tgatgcccac cagcggcgcg aataccccgg tggtggcgca
     9121 gctgagctct tcggtgtcgt cggcctcggg aaacaggcag tggtagcacg gcgcctcgtc
     9181 atcgcgcagg tcgtacacgc tgacctggcc gtcgaagcga atggcggcgc cggacaccag
     9241 cggcttgcgg tgcttcacgc aggcgcgatt gatggcatgg cgggtggcga aattgtcggt
     9301 gcaatcgagc acgatgtcgg cggcggcgac cgcctcgtcc agggcggcgc ctgccaggcg
     9361 cgcgacgcgc gcatcgaccc gggtttccgg gttgagcgca accagcatgt cgcggcccga
     9421 ctccgccttg ggacggccca ggctggccgt ggtgtgcagg atctggcgct ggaggttgct
     9481 gagctcgacc tggtcgtcgt cggccagggt gatgcggccg acgccggccg tggccaggta
     9541 cagggccgcg ggcgagccca ggccgcccgc gccgacgacc agcacctgtg cggcgaggag
     9601 tttttcctgc ccttcgatac ccagctcgtc gagcaggatg tggcgggcat agcgcagcag
     9661 ttgctggtcg ttcacggtgg cgggcggccc ggcctggcgg ccgggccttc ctggcttact
     9721 tggccggtgc gggctcgacg ccggacggcg tgatcgtcag gcgctgcgcg ccgtcggtct
     9781 tgacgtcgcc gggcttgacg tcgccgggct tggcattcgc ggcattgaca tcggccttgg
     9841 ccgtggtcaa cgcgcccttg tcgatcggct tgccggccag caggttcatg gcctgctgca
     9901 gttggaagtc gtccttgccg ccgaactcga agatcttggc ggcgggctga tcggccgtcg
     9961 actcgtcgcc cttgacttcg ccgccaccca ggggattggc cagatggtgc tgcaagtcgg
    10021 cctcgcgcgg caggcggaac aggtcgccct gcgcggtatc ggccacgacg tactcgggca
    10081 cgatgccggt ggcctggatg gagcggccct tgggcgtgaa gtagcgcgag gtggtgagct
    10141 tgatcgccgt gtcctgcgac agcggcagga tgacctgcac cgaacccttg ccgaagctgc
    10201 gattgcccat gagcttggcg cgcttgtaat cctgcagcgc gccggccacg atctcggaag
    10261 ccgaggccga acccacgttg atcagcacca ccatgggcac cgtcttggcc caggcgggca
    10321 cgtcggacag gtagttgcct tcgccgcggg cgtattcgct ggggacggcc aggtacttgt
    10381 ggcgcgcgtc ggctacgcga ccgtcggtgg acaccaccag cgaatcgggc ggcaggaagg
    10441 cgcccgccga accgatggcg ctggtcagca ggccgcccgg gtcgttgcgc aggtcgagca
    10501 ccaggccctt gggcgcgccc ttggcgccga ggtccttcag gttcttgacc aggtcggagc
    10561 cggtgcgttc ctggaactgg gagatgcgca cataagccac gccgccgtcg agcatcttgc
    10621 tgcgcacgct gcgcaccttg atgatgtcgc gctgcatctt gatcacgatg ggctggggct
    10681 ggtcggcgcg catgatggtg agcgtgatgg gcgacttggg cgcgccgcgc atgagcttga
    10741 cggcgtcgtt cagcgccatg cccttggtga actggtcgtc gatcttggtg atgagatcgc
    10801 ccgccaggac gccggcgcgc gcggccgggg tgtcctcgat gggcgagatc accttgacga
    10861 agccgtcctc ggcgccgacc tcgatgccca ggccgccgaa ttcgccctgg gtggtggtct
    10921 gcatgtcctt gaaggcgtcg gcgtcgagat aggccgaatg cggatcgagg ttggacacca
    10981 tgcccgagat ggcattgttg atcagggtct tgtcgtcgac cggctcgacg tagttgtcct
    11041 tgatcgccgc gaacacgctg cttagctggc gcagctcatc gagcggcagc ggtgcgcctg
    11101 ctcgctgcgc gatggcgctg acgccggcgc tgagcaggac gcctgccacg gcccccatga
    11161 cgatcaagct ggccgcgcgg acgcgtcggc gcgccggctt gatacgggat tcttgactgc
    11221 gggttggttc gttgcgcttg ctggtgctca tgcacacttc ctgagttgag gcccgtagcc
    11281 atagcctaca gggccagcca ctgtgccggg tcgaccggcg taccgcggta gcgaatttca
    11341 aagtataggc ccgattccac ctggccgccg gtggcgccga ccgatgcgat agtatcgccg
    11401 gccgctaccg ggtcgcccac gcgcttgagc aggctctggt tataagcata aacggtcaaa
    11461 tattgctgtc catggtcgat gatgatcaag ttgccgaaac cacgcagcca gtcggcatag
    11521 accaccaccc cggcagccac cgccttgacc ggcgttccct cgggggcgcg cagcacgatg
    11581 ccgcgccata cgccgccgtc gggtcgcccc acgccgaagc ggccctgggg cgtgccgccc
    11641 gccacgggca ggggcaggcc gtgcttgagg cccgtgccgg tgacgtgggg gcgccgtttc
    11701 ggcgcgtcgt cctcgtcgtc cggttcttcg gcctgggcga cggcggcggg ccggctgtcg
    11761 ggcttgtcgg ccgagggcgc gggagagggg ggtggcgggg aaaccggagc cggggcggga
    11821 accggggccg gcgtcggctg ctgcagctcg ggcggcgtca ggcgcggtgt ggcggattcg
    11881 gtcaggcggg gccgcaggcc ggccgcatcc gggtcggcca gcgccacgct gccgtcctgg
    11941 cccgggcgag ccgcggcggc cgcgcgctcg gcctgctcgc gggcctgggc cgcctcgttg
    12001 gccgcggccg agcggcgcgc ctggtcggcg gcctggcggg cctgcctggc ctcgtccgcc
    12061 ttgcgcgcgg cgtccgccac tttgcgggcg gcctcggcct tgcgggcctc ttccgccttg
    12121 cgcgcttctt ccgctttgcg ggcttcctcg gccttgcgcg ctgcttcggc ccggcgcgcc
    12181 tcctcggccg cctggcgggc gacggccacc tgcaggtcgt cgatcaggtg cgacaggcgc
    12241 tggtcgtcgc ggcccagctt gccggcctcg gcccgctggg ccgcaagctg gccttcgatg
    12301 cgggccacca cggcggcgcg ctctttctgc tcggccagca ggttgttctt ctgggtcgtg
    12361 gtttcgtcgg ccacgcgcgc caggtcggcg cggcgggccg cggcggccga ctgcagctgg
    12421 gccatgcggt cgaggtcggc gcgcagcgcg gccagcgccc gggcccgggc ctgcgagacg
    12481 aagtccaggt agcccaggtt gcggcccagt tgctgggggt cgtcgcccga cagcagggtc
    12541 gcccagggcg acaggccgct ggaatactgg gcgcgcagct ggcgggccag ctcttcgcgg
    12601 cgctggccca gcacggcctg ctgggccgtg atctgcttct cgatgccggc cagctcgctt
    12661 tcggcgcggc gctgggcctc gcccaggtcg cgcagctgca ggttgatgcg ggagatggcg
    12721 gtctcggagg cgcgcagcgc gtcggcggcg tccttgcggg acgcctcgcg cgtgtctatc
    12781 gctttttgca gggcgttgat gcgctcgcgc agggccgcct gctcctgttc ggcctcggtc
    12841 tggcggccag ccaggtcgga cgtcgtggcc gcgccggccg atgcggcagc cagcgccagc
    12901 gcggccaccc acagacttcc tgccatccag cgcatcgtcg ttgtccgctt gctttgctta
    12961 ggtgcgattg tgcgcgatta cttcttggcc ttgccttgcg ccgccacggc ggccatggcc
    13021 gcctcgatgg cggcgggatc gcccaggtag tagtggcgga tgggcttgag gtcgtcgtcg
    13081 agctcgtaca ccagcggctg gccggtgggg atgttcaggc cgacgatgtc gtcttccgac
    13141 atattgtcca ggtatttgat gagcgcgcgc aggctgttgc cgtgggcgga aattagcacc
    13201 ttgcggccgg cgcggatggc cggcgcgatg gagtcgttcc agaagggcag cacgcgggcc
    13261 acggtgtcct tgaggcactc ggtggccggc agctcgccgg ccggcacgcg ggcgtagcgc
    13321 gggtcgaagc gcgggtggcg cggatcgtcc tcgcccaggg catcgggcgc gatggcgtag
    13381 gcgcggcgcc agaccagcac ctgttcgtcg ccgaacttgg ccgcggtctc ggccttgttc
    13441 aggccctgca gggcgccgta gtggcgctcg ttcaggcgcc agctgttgcc caccggggtg
    13501 tacatggcgt ccatggcgtc gagcgcgatc cagagggtgc ggatggcgcg cttgagcacc
    13561 gaggtgaagg cgatgtcgaa ggtgtagcct tcctgcttga gcagcaggcc ggcctggcgg
    13621 gcctgctcgt ggccgaggtc ggtcaggtcg acgtcggtcc agccggtgaa ccggttttca
    13681 aggttccatt ggctttcgcc atggcgcatc agaacgagtt tgtgcatggt ggtagggccg
    13741 cgccgggcgc ggaaaatgag tggaaaatcc gggggcaatg gggcgtgctt tgcactcatt
    13801 ttataattga ctactttgcc ggggttgttt caccccgaat tttcccgcgt tagcagccgc
    13861 ggcttccctt caggaccaat tcgtggactt cgtggatctt ctgcatttcc tgctcgacaa
    13921 gagcaacatc atcatcgtgg ccgtcgccgt cgtctcgggg gtgatgctgg ccctgcccaa
    13981 tctgcgccgc agccgcggcg gcgcggcgct cagcacgaac gaggccatcc agatgagcaa
    14041 ccagcgccag gccgtgtggg tcgacgtgcg ccccgcggaa caattccagg ccggccacat
    14101 cgcccaggcc cgcagcctgc cggccgccga cgtcgagcaa aaaaccgcca gcctgcccaa
    14161 gaacaagccg ctcatcctgg tgtgcgagca gggcaaggac gccccgcgca tcgccgcgcg
    14221 cctgcgcgcc cagggctatg ccgacgccac cgcgctggaa ggcggcatgc gcgcctggtt
    14281 cacggccggc ctgcccgtga cgcaaaagag ctgaggcttt tccccttcta tcggatcgac
    14341 catgcaaaaa gttgtcatgt acagcaagga ttattgtcct tattgcgccc gagccgaagc
    14401 gctgctgatg gaacgcggcg tgaccgactt cgaaaaaatc cgcatcgacc atgaacccgg
    14461 ccagcgcgag gccatgatcg agcgcaccgg ccggcgcacc gtgccgcaga tctacatcgg
    14521 cgcaacccac gtcggcggct gcgacgacct ggtggccctg gaccgccagg gcggcctgct
    14581 gccgctgctc aacggctgaa cccagccccc cggcactctt ctttatccgc acccatacaa
    14641 cggaacttcc atggctgacc aaaacaccca gcaagaatca ggcgctcctg ccttcaacat
    14701 gcagcgcgtc tatctgaaag acctctcgct ggaaatgccc aacgcgccgc acatcttcct
    14761 ggaacaggaa acaccgcagg tcgaggtcag cgtcaatgtc ggcggccagc gcctggccga
    14821 cacgatcttc gaatcgaccg tcaccgccac cgtcacgacc cgcatcggcg acaaggtcct
    14881 gtacctggtc gaggccaccc aggccgccat tttcgagctg gccaacatcc cgcccgagca
    14941 gatggacccc atcctgggca tcgtgtgccc gaccatgctc tacccctacc tgcgcgccag
    15001 cgtggccgat gccatcaccc gcacttcgat gcccccgctg cacttggccg aagtgaactt
    15061 ccaggcgctg tacgaacagc agatggcgca gcaaccgggc cagtccaacg gcaacgactc
    15121 cggcatcatt ctgccgccgg gcgccacgcg ccagtgacgg gcctgcccgg cagcgcgctc
    15181 gccatgaaca cgcccaccgc attgcgcgtc gtcgtgctcg gcgcgggaag ctggggcacg
    15241 gcgctggccg ccgccgcggc gcggcgccat cccaccctgc tctgggcgcg caacgccgcg
    15301 caggcgcaag ccatggcgag cgagcacgaa aacagccgct acctgcccgg cgtcgccctg
    15361 cccgaatcgc tggccatcga aagcgatctc gccgccgcgc tggcctttct gcaaggcggt
    15421 ccggcgcagc cggccaaccc cgggctgatc atcctgggcg tgccggtggc cggcctggaa
    15481 gaggcctgcc gcgcgctggc ccggcacctg tcggccatgg acccggccgg cgccgcggcg
    15541 catgtgccgg tggtctggac ctgcaaggga ttcgaagaga ccaccgcgcg gctgccgcac
    15601 gaaatcgccc aggccgccct gcccgccggc tggcgcggcg gcgtgctctc gggcccctcg
    15661 tttgcgcgcg aagtggcgca aggcctgccg gtggcgctca ccatcgccag ccaggagccg
    15721 gctgtgcgcg atacggccat cgcggccctg catggcgggc cgatgcgcgt ctatgccagc
    15781 accgacgtcg tgggcgtgga agtcggcggc gcgttgaaga acgtcatcgc ggtggcctgc
    15841 ggcattgccg acggcctgcg cctgggcacc aatacccgcg ccgcgctgat cacccgcggc
    15901 ctggccgaga tctcccgttt cggccaggcg ctgggcgccc aggccgagac cttcgcgggc
    15961 ctcaccggcc tgggcgacct ggtgctgacg gccaccggcg acctctcccg caaccgccgc
    16021 gtgggcctgg aaatcggcgc cggccgcacg ctggccgaca tcctggccag cggcatcacc
    16081 gccgaaggcg tgcgttgcgc gcgcgccgtg ctcgcccggg cccgccagat gaacgtggaa
    16141 ctgcccatca ccgaagccgt gtgcgccgtg ctgttcgacg gcgtggctcc ggcgacggcg
    16201 gtgtcggcgc tcctggcgcg cgaagtccgg tacgaatcct gagccgctct cctgtggccc
    16261 ggctgtaggc tgtaatgtcc tgaaaccccc aaggcggccc gcgcttcagg ttcggcgccg
    16321 aacgggagta cattagtctt gaaacgcatt gcgcgcagcg cgcgcacgca gccggcggcg
    16381 ggtcttgccg gcaggagtca caatatgaag aattccacgc tcatcaaaac cgctgccctg
    16441 gcgctcatcg tcagcctggg agccgcctgc ggcacggccg aggcctgggg ccgctatggc
    16501 ggccacggcg gttatggcgg ccacggcggc tattaccacc gcggccccaa cggctggttc
    16561 atcggcacgg ccctgggcct ggctgccgcg ggcaccgtca tcgccctgag ccaaccgcaa
    16621 tacgtgtacc cggcgccagt ctatgccgcg cccgtgtatc ccgccgcgcc ggtctatgcc
    16681 gcaccgcccg tggtgcaata cgcgccgcag ccgcaacaag ccatggcctc gaccgacgtg
    16741 atcgcctatc ccacgcgggg ccagaatccc cagcagcagg ccaacgaccg ctccgcctgc
    16801 gagcagtggg ccgccaacca gagcggctac gaccccgccc aggcttcgcg gtggaccacc
    16861 accgtgcaaa ccgattcgta ctcgcgctcc atcggcgcct gcatgaaggg ccgcggctac
    16921 agcatcaact aagcgcctga gcgcgcgggc gcgggccgca tcgagcgcgc cagcaccggc
    16981 cgggccagcg aggcccggtc acaccccgcc cgcatagccc tgctggcgcc aggcctcgaa
    17041 taccgtcacg gccaccgcgt tggacaagtt caggctgcgc tggccgggcc gcatgggcag
    17101 gcgcaagcgc tgcgcctggt cgaacaaggc cagatgctcg gccgacaggc cggcggtctc
    17161 gcggccgaac acgaacacat cccccggctg gaaaaccgcc tcccccacgt gatgcgtggc
    17221 gcgcgtcgtc atggcgtaga tgcgccgcgc aaccgcgccc gtggcctgca gggcctgctg
    17281 caggctgtcg tgcacctgca ccggctgcca ctcgtgatag tccagcccgg cgcggcgcag
    17341 cctggcgtcg tccaggtcga aacccaaggg acgcaccagg tgcagctgcg cgccgctgtt
    17401 ggcgcacaag cggatggcat tgcccgtgtt gggcgggatt tcggggcaaa cgagcacgat
    17461 atggaacatg ccgccattat ctcaaagcgg cctgggcgtg cgtgccgcca cccagaccga
    17521 cacgcttgcc gcccccgcgg ccagcaaggc ccgcgcggcg gcgtcggccg tgctgccggt
    17581 cgtcatcacg tcgtccacca gcgcgatgcg caggccgtcg agccggcgcg tgcagaggta
    17641 ggcatcgagc gccgccgcct cgcgttcgga ccgggccagg ctggcctggc gcacgccggt
    17701 ttcgcggcac cgccgcaaga cgccgcgcag caggcgcagt tccaggcggc gggacaaggc
    17761 tcttgccagc tcgccggccg ggttgaagcc gcgctggcgc aaggaggcgc ggctggccgg
    17821 caccggcacc acggcatcga tgggcagacg gccggtccgc ggccccggcg cacgggccgc
    17881 ctcggcggtc cgcacggcct gggccagcac ctgcgccagg gcgcccgcca cgacatggcg
    17941 ccgcgcgccc ttgtagcgcg cgatcaaccg gtcgaagggg tccgcatagt cgaacgcggc
    18001 cacggcccgg acaaaggccg gaggatacag ggcgcattcg acgcacaagc cgccatgccg
    18061 cccggcctgc ccggcgctca aatacacggc gcagcgcggg cagcgccgtg ccgcggcctg
    18121 ctcgagcacg gcctgggcgc aaggctggca cagcagtccg ccgcgcgccg cgccctcgca
    18181 ggcggggcag tcggagccga tgccggccag caggctgcgc cagcgccgct ccaggccgcg
    18241 ccacaaggcc ggcaaggccc cggcgcgcgg atgcgggata atgcgggtaa ctgtcacacg
    18301 acgctcacca gatgccacga gccggtcctc ctgaatcaga caccggcccg catcacatca
    18361 tgccagccgc cgggccgcca ggcccggtgc gatagaaatg tccctgcccg cttcgccgct
    18421 gcccaccctg cccctctcga ccgcgcacgt ccagcgccag ttcgcccgcc gcggcgacct
    18481 ggccgaggcc cagtttctct acggcgaagt cgcccgccgc atgctgggac ggctgtccta
    18541 tatccgcctc gatcccaagc agatgctcga tgccggctgc ggcgcgggcg acaacctggc
    18601 gctgctgcgc gagcgctacc cgcaggccgc ctacatcggc ctggatcatt gcgaaccctt
    18661 gctggcgcat acgcgccgcc gcttcatggc ggccggcatg ggcgcctggc tgggccggct
    18721 ggtaggaaat aagcagccgg cgctgcaata cgtgcgggcc gacctggccg acacgggcct
    18781 ggcgcccgag tcgctggacc tggtctggtc caacctggcc ctgcactggc accccgagcc
    18841 ccacgccgcg ctggccgaat ggcgccgcgt gctgcgcgtg ggcgggctgg ccatgttttc
    18901 ctgcctgggc ccgggcaccc tgcgcgaatt gcgccaggcc ctggccgacg ccggcctgcg
    18961 caccgccacg cccggtttcg tggacatgca cgacttcggc gacctgctgc tcgagaacgg
    19021 ctatgccgat ccggtgatgg atcaggaaac cctcaccctc acctacgaga cgccgcagaa
    19081 gctgctggcc gatgtctggg cgctgggcgg caacccggcc accggccggc gcgccggcct
    19141 ggccggccgc gcctggcgcg agcgcctatg cgaagccctg gccgcgcagc gcggccagga
    19201 cggccgcatc gcgctgagca tcgaggtggc ctacggccac gcctggcggg ccgcggcgcg
    19261 gcgcgccgcc accggcgaga cccgcgtctc gctcgaagcc atcggcggac ggcgggcgaa
    19321 gtaggggttg cgcaatggcg gccgcgcctc agtgcagcat gggttcggaa tgcgcccgca
    19381 cccggcggcg tccgtaggcc gagtcgacca gggcgccgga tgccgcgccg ccgccgcgcc
    19441 acagccgcgt catgctctcc accaccccgg gcagttcgcg caggcgatgg aactcggcgc
    19501 gcagccagcg cgcatcgttg ccgccgcgct gcaggtcgtc gcgcagcgcc tgcagcatgt
    19561 ccaccgtgcc cagctcctcg gccacgggca ccaactgctg cagcagcagc cgcaggtgat
    19621 cgcccagccg cacgcgttcg ccttgcggcg tgacgtaggc gccatgcagg ccgaaacggc
    19681 aggcctggaa gtggttgctg cgataggcca gccaggccgt ctcgtccggc tcttccgtgc
    19741 gcgacaccag caccgccagg gcttgcgcga aagcggccag ctggcaggcg cgctccacgg
    19801 tcagcggcgt gtcgcagacg cggatctcca ccgtgccgaa ttcgggcttg gggcggatat
    19861 cccagtacag gtccttgatg ttctcggcca ggcccgtggc gcgcagctgc gcgatatggg
    19921 cctcgaagcc gtaccagtcc cgcacgtcgg ccggcatgtg tcccgacagg ggaaagttgt
    19981 tgaccgcgtt gaggcggcag cacgaataca gcgtgtccac cccctcgaaa tacggcgagg
    20041 cggccgacag ggcgatgaag tgcggcacat agggcgacag gccgcgcacc agccggacgg
    20101 cggcgtcgcc cgaggtcgtg cccaggtgaa tgtgctggcc gaacaccgtg aactggcgcg
    20161 ccaggtagcc gtaggtctcg gcgatgtaat ggaagcgcgg gctgtcgtgg atgcagcggt
    20221 cctgccagcg catgaagggg tgggcgccgc cgcccgacac ggcaatgccc agggcgtcgg
    20281 cggccgcgcc caccacgtcg cgcatctcgc gcatctccgc cagcaggccg gcggggtgct
    20341 cgtgcaccga ggagttgagc tcgatcatcg aacgggtgat ttcgggcttg accctgtcgg
    20401 ccatgcaatg gccggccagt tgggccagca aatcgtcgga ggcggcggtc aggtcgaagc
    20461 tggtcgggtc gatgagctgc agctcgagct cgatgcccag ggtgttgggg gccgacgagg
    20521 cgaagggaag cgattccatc tcggtctcct tgaatattgg tcggaaatgg ccctgccagg
    20581 acttgaggcc cggggtattc acgcccccgg ggtgcctggc ggatggactt gccgttcgat
    20641 agtaaacggc caattgatta cagaacgact ctaaatcgtg acatttgtgt gcagtacgca
    20701 gaatcacggt tttcccccag gctggcaggt aagcgaatgc tcacctatgc aagcggatca
    20761 ggggcgagcc ggtcccatga ggtcggccgg ctggtcgata tcggccagca cgcccggatc
    20821 gcccgtctcg gccaggacga ctccatggcg ggtcagcagg ccgcgggcgc cctcgtctcc
    20881 cgccagggcg gccagttcgg gccagagcgc cgcggcgaac gacacggggt ggccgcgccg
    20941 cccttggcag gcggccgcga cgatgggatg cccgcatccg gactcggcga cccggacgtg
    21001 gctggccggc gcgacccagg gcatgtcggc cagggcgacc aggcagtggg tcggcgcgag
    21061 cgccgccgcg gcgcccgcct gcgccagcag ggcctcggcc gccgccgcca ggctgccccc
    21121 catgccttcg cgggcacgcc cggtgcgcag cacccggcac cccatgcccg ccagcaggcc
    21181 ggccagcgcc tggctttcgg gccgcaccac cgcccagacg ccaggcagga cggccagcat
    21241 ggccgccgcc gacgcctgcg ccaccggacg gccatccggc agcgaggcca gcagcttgtc
    21301 gccgcccccg gccgcctgcg catagcggcg gccgtagccg cccgccagca ggatgcccat
    21361 gcaccccgcc atgccgcttg cctggaccgt gccggcacgc atgtcagcgc cgccagaaca
    21421 ggggggtgaa caagcgcggc accgtcggca gcgccagccg gccgatcatg atgttgcgtt
    21481 cttgaagata gcctggcggg gcaggctcgg tatcgatcgc cgcgatgtcg ttctgcaccg
    21541 gcaccaggtt cgcggccacg cgggtcccca ggcggccggc gcccaggatg gggatcttca
    21601 tgtgctgcgc ttgcgcacgg attcgatgcc gagttgcttg agcttgcgat agaggtgggt
    21661 gcgttccagg ccggtgcgct cggaaacgcg ggtcatgctg tggttttcac ggaccaggtg
    21721 gtattcgaag tagatgcgct cgaaggcgtc gcgcgcttcg cgcaggggca ggtccagcga
    21781 gatattgccc agctggccgt tgcccgtctg ggcttgcgcc tcggccacgg gcgccgcggg
    21841 cgggggatcg agttcgtctt cgagcggcac gaccaccggc gccagggccg gggcagccga
    21901 ggccggatac ggcgcgcggc cgcgcgacaa gcccgacgag atggtcttga gcaggcgctg
    21961 cagggtgatg ggcttttcga ggaaatccat cgcgccgatg cgggtggcct cgacggcggt
    22021 gtcgatggtg gcgtggccgc tcatcatgat gacgggcatg tcgagcaggc cctgcgagcc
    22081 ccattccttg agcaggctca cgccgtcggt atcgggcatc cagatgtcga gcaggaccag
    22141 atcgggccgc atgcgcaggc gcgcagcgcg cgcctcggcg gcattttccg cgagttcgac
    22201 cgtgtgtcct tcgtcgtaaa gaatctcgga cagcagttcg cgaatgccaa cttcgtcgtc
    22261 gaccaccaga atcctggcca tagacctctc acctattgcg tcgccgcatt atcgctcttt
    22321 ttgccggcct tttttattcg gcggccaggc gagtcagcag gatggaaacc ctggccccgc
    22381 cctccttgcg attggcaaga tcaattcggc catcatgctc gtcgatgatc ttgcgcacga
    22441 tagccaatcc taacccagtt ccgtgcgcct tggtcgtgac ataaggttcg aaagcccgct
    22501 gcgccacctg ggccgcgaag cccggcccat tgtcagatac cgtaaagcgc acggcccgct
    22561 cgctggtgcc gtcggaccgg gcgctctgca ccagctgggt tgacaccctc accaggccgg
    22621 cctcgcccag gtcggtcacg gcatcccggg cgttggacag caggttgtgg atgacctggc
    22681 gcagctgcgt ggggtcgcct tcgatatcgg gcaggccgga ctcgagcctc acatccaggt
    22741 tgagcgcccg cggcccttcg ccgccgcccg cctcccagcc gtacagggcc agcacgtcgg
    22801 ccaccaggtt gttgaagacg atgcgctgca ttaccgccgg cggcgtgcgg gcgtattcgc
    22861 ggaagtcatc caccattttc ttcagcgagc cgacctggtt gacgatggtg ttggtggcgc
    22921 gggtcagcat ctgggcgtcg gccggctcga gccggccggc cagcttcatg gccagccgct
    22981 cggccgagag ctggatgggc gtgagcgggt tcttgatctc gtgcgcgagg cgccgcgcca
    23041 cctcgcccca ggccacggtg cggctggccg agatgacctc ggtaatgtcg tcgaacacca
    23101 ccaggtagcc gttgccgcgg ccgtccacgt gcaggtgggt gccgcgcgcc agcaaggtca
    23161 ccgcctcgcc ctgcgtgccc cgcttgatct cgaactgctg ctgccagtgg gtgcgttcgg
    23221 accccaccgc ggcgtgctcg gaaaaggcct ggcggacaat gttggcgaac tcgatcatgc
    23281 cgtcggcggt ctcgagcggc cggccgatca ccgagcgcag atcagcctgc aggatggtct
    23341 gcgcgccctg gttgacggtg gtcacgcgga acccttcgtc gaaggccagc acgccggaag
    23401 acaggttggc cagcacgctt tccaggtaga tattggagcg ctccagctgg cgccggttgc
    23461 tctcgaccat gcggcgcgcc tcgtcgagct ggcgcgtcat ggcgttgaac gaacgcgtga
    23521 gctggccgac ctcgtcgcgc tcgggcggct cgggcagggg ccggtagtcg cccacgctca
    23581 cggcctgcgt gcccgccgcc aggcgcagca gcggccgcac caggcgcttg gacaaggaca
    23641 gcgccaccgc gatggacgcc agcatggcca gcagcagcgc cagcgtgagg gtgatgccgt
    23701 agagcttgcg caagcccagg cgcgacaggg ccagctcctg gtagtcgccg aagccctgct
    23761 gcacgcggtt ggcgttgtgc gccagctggt cgggcaccgg ctgcaccagc tgcatccagc
    23821 gcggctcgga cgccgtgccc aggccgctgt cgaaacgcac cggcgtcacc agggggacca
    23881 ccacccgcag gagcagggtg ctttccgcgc cccgcacggc aggctcggac gcctcggcgg
    23941 ccgaatagcc gcccgccagc ttgagctggt tcatcacggt cgacggcggc agggccggca
    24001 gcagctggcc gtactggctg gtggagaacg ccaccatccg cccgctgctg gtgaacacca
    24061 gggcttcctg cacgccgttg ttctcgcgca gccgggtcag ggccagcgag acgtcgttgt
    24121 cgttgaggtt gttgagctcg ctggccatgg cgcgggcgcg cgagttgagg tcggcaagct
    24181 gcgtgtcgag caccgcgcgg cccaggttca ggccggcctc cagcgcgctg tcgacccgca
    24241 cgttgaacca ggactcgatc gagcgcgaca ggaactgcac cgacagggtg tagatcagcg
    24301 cccccggcac caccccgatc agggcgaagg ccagcgaaaa gcgcgccgtg agccgggcgc
    24361 cgaactggcg gcggcggatc tgcttgacca gtcggccggt cagaccgacc acccaggcca
    24421 gcagggccag ggcgaagatg ccgttgagca gcagcagcac gtcgtagtag cgggcaaagc
    24481 gcgaggcatt gccggtggac caggccaaga ggcccagcag gcccaggccg ctcaagccgc
    24541 cgatgatgag cacaaaacgc aggaagcggg tcatgtgagc gtgggggcca tcacttctcg
    24601 ggccgcgcca gggcgaagga aaaatcggtc cacggcgtgg cgggcgacca tgaggtgctg
    24661 ttcaacgcgt tgacctggaa aggccgcgtc aacatggaag tgtccagccg cacgcgcagc
    24721 tggccggcat actgcgtgtc ggagtcgaat ttttcagtcg tgctgacctg ccagttgcgg
    24781 atgtgccgca ccacgtccat ggcctcgtcg agcgaggcca cgggcaggga cagctcgccc
    24841 gcgccggcgc gccattggcg ggtcagcgcg ttgtaaacga tgcgccaggt catcgaggtg
    24901 ttgaccacgg tatggtcgaa ccaccaccag cgcgaacgcg tgatgacgag gtcggccgtg
    24961 aagaacaagg gcacgccgcg ctgcgcggcg ttgcgcagct ggtcgttgag gtcgaaacgc
    25021 acgtcggcgt cgatctcgag ccggccttcg tgcacggagg gatcgatgcg ctcgatgcgc
    25081 gggtcggcgg cttgcgcgac ctggcctgcc gacaccccga aaaggaaaat tgccagcagg
    25141 aaagcccaaa aaccgcgcga gatcgtcaaa gaaggacagg cacgtggcgt catgtcacct
    25201 attcttggcg attcatccct gcttggcaaa cagggcatag aaaaaaccgt catgccgggc
    25261 ggccggtgtt gcgccgatgg caacgggcag cagctgtccc ggcgcgggca gccgccgcgc
    25321 ctcggcatgg cgggcctgga aagcccgcgc ctggacctcg ccctcggccg ggaagatgga
    25381 acaggtgaca tatagcagcc gcccgcccgg ggcgacggtg cgccacaggg catcggcgat
    25441 ccgggcctgc agcccggccg tgcgcgccac atcgtcggcg cggcgcagcc agcggatgtc
    25501 cggatggcgg cgcacgatgc ccgacgccgt gcagggcacg tcggccagca cggcgtcgaa
    25561 ggggcggccg tcccaccagg catcggggtc ggccgcatcg gcctgcttca atgccacgcg
    25621 cggcgtgcgc aagcccaggc ggtccaggtt ctggcccacg cgccccaggc gcccggcatc
    25681 gacgtccagc gccagcagct ccaggtcggc cagctccagc aggtgcgccg tcttgccgcc
    25741 cggcgcggcg caggcgtcca gcacccgcat gccgtcgcgc ggcgccagga gcggcgcggc
    25801 cagctgcgcg ccggcgtcct gcaccgacca ccagccctgg gcgaaacccg gcaactgcgc
    25861 caccggccgg ggcgcggcca ggaccagccc ggcctcgccc gcgggttcgg ccgcgatgcc
    25921 ctcggcctgg aaggccgcca ggacctgctc gcgcgccgcg cgccgccggt tgacccgcag
    25981 cgtgagcggc gcgggcacgt tggcggcctc gagcagttcg gcgtagcgat cgggataatc
    26041 ggccttcagg cggtccaccc accatggcgg atggttccag cgcgcctgcg gcctgtcggc
    26101 cacggcccgc tccagcgcct cctgctcgcg caggaagcgg cgcaggcagg cattgagcaa
    26161 gcccttgtac ggcgccagct tgcgctcgcc gtcggccgcc tgcacggcct ggttcaccac
    26221 cgtgtgcggc gcataggacg gcatgccggg caaaggctcg gccgcgggct tgagcagggt
    26281 gagcgccacg cgcagcagcg cgcccaggcg cgcgccgggc gatttctcga ggagttcgcc
    26341 ggcgatctcg tcggcccagc ccagctggcg catggcatga aagcccaggg cctgcgtgcc
    26401 cggccgcagg gcggcgtcga cctgggccag ggcctcggtc agcgaacggc cctgcaggac
    26461 ggcctggacg gcgtcggcgg cggccaggag cagctgcgac agggggggag tcaggttttg
    26521 cggcatagcg caattgaagc atgagcggag gccgcggctt gcggcacgcc ctggccgcac
    26581 cctgcccgca ggtacaataa gggcttgata ggaaaccgat ttatgtccac cgtcaccgcc
    26641 cccaaggtcg gctttgtctc cctgggctgt cccaaggccc tggtcgactc cgagcgcatc
    26701 ctcacgcaac tgcgcaccga gggctaccag atcaccccca gctacgacga cgccgacgtc
    26761 gtcgtggtca acacctgcgg cttcatcgac agcgccaagg ccgagtcgct cgaggccatc
    26821 ggcgaagcca tcgccgagaa cggcaaggtt atcgtcaccg gctgcatggg cgtggaagag
    26881 tcggtcatcc gcggcgtgca ccccagcgtg ctggccgtga ccggcccgca gcagtacgag
    26941 caggtggtgc gcgccgtgca cgatgccgcg ccccccaggg ccgaccacga ccccttcgtc
    27001 gacctggtgc ccccgcaggg cgtcaagctc acgccgcgcc actacgccta cctgaagatt
    27061 tccgagggct gcaaccaccg ctgcagcttc tgcatcatcc cctcgatgcg cggcgacctg
    27121 gtcagccgcc ccgtgggcga cgtgctcaac gaggccgagc gcctggtgcg cgccggcgtg
    27181 aaggagctgc tggtgatctc gcaggacacc agcgcctacg gcgtcgacct caagtaccgc
    27241 agcggcttct ggaacggccg ccccgtcaag acccgcatga ccgagctctg cgcggccctg
    27301 tccgagctgg gcgtgtggac gcgcctgcac tacgtctacc cctatccgca cgtcgacgag
    27361 gtcatcccgc tgatggccga gggcaagatc ctgccctacc tcgacattcc cttccagcac
    27421 gccagcccgc gcgtgctcaa ggccatgaag cgcccggcct tcgaagacaa gaccatggcg
    27481 cgcatcaagc agtggcgcgc ggcctgcccc gacctcacca tccgctcgac cttcatcgtc
    27541 ggcttccccg gcgaaaccga agaagacttc cagtacctgc tcgactggat gaccgaagcc
    27601 cagctcgacc gcgtgggctg cttccagtac tcgcccgtgg aaggcgcgcc ggccaatgcg
    27661 ctgggcggcc acgtgcccga cgagctcaag caggagcgct gggagcgctt catggcgcac
    27721 cagcaagcca tctcggccgc gcgcctctcg gccaaggtgg gccgcgagat cgacgtgctg
    27781 atcgacgggc cggacgagga cggcgacatc gtcggccgct cgagtgccga cgcgcccgag
    27841 atcgacggcc tggtgtacgt ggacagcgcc aaggcgctga aagccggcga catggtgcgc
    27901 gtgcgcgtga ccgacgccga cgagtacgac ctctggggcg aaacgctgta atgcccgatc
    27961 ggctgcatgc ccgcgcgcgg caactcatcg cccagctcgg cctgcagccc catccggaag
    28021 gcggccacta ccgcgagatt ttccgctccg gcctgcaggt ccggcgccag ccggacgggc
    28081 tggcgcgcag cgcgctgact tccattttct ttttgctgcc gcgcggcgcc tgcagccgct
    28141 ggcaccgcgt ggcccaggcc gacgaggcct ggcaccacta tgagggcgac cccatcgccc
    28201 tgcacgtgct gctgcccggc gcccgccagg ccgagacctg gctgctcggt cccctcggcg
    28261 ccggccaccc cgatgcgctg cccgcgcgga tcgtgcccgc caatgcctgg caggccgcgc
    28321 agcccctggg cgactatgcc ctcgtgggct gcagcgtggg ccccggcttc gagttcgccg
    28381 acttcgccat ggcgcgcgat ctgcccgccg ccgagcggcc cgcggcactg gacgcggcgt
    28441 ttctataaac tatggccatg catgcgctgc cccatcagcg cccggatcac tcagcatggc
    28501 tttagaaaac atctgcgttt actgcggctc caacaagggc cgcgaccccc tctacatcga
    28561 acgagcccag gccttcggcc gcgaactggc gcgccgcggc ctcggcctgg tgtacggcgg
    28621 cgccgacgtc ggcatcatgg gcgccgtggc cgacaccgtg ctggccgcgg gcggccgcgt
    28681 catcggcgtc atccccgaat ccctgcgcac caaggaaatg gcccaccagg gcctgaccga
    28741 gctgcacgtg gtgcccacca tgcacgagcg caagcagctc atggccgaga aggccgacgg
    28801 cttcgtcgcc ctgcccggcg gcgccggcac cctcgatgag atcttcgaag tgtggacctg
    28861 ggcgcaactg ggctttcacc gcaaaccttg cggcctgttc aacgtggcgg gctacttcga
    28921 ccagctcgcg gcctttctcg accacgcggc cggagaaggc ttcatgcggg ccgagcaccg
    28981 cgccatgctg atggtggagt ccgaccccgc cgccctgctg gaccgatacg ccgcctacca
    29041 cgcgcccacc gtgtccaagt ggatagccac cggaggccgc tgaccatgca agaccagatc
    29101 ccccacgaca cggccgccgt cctgcgcgac tacttccacg ccaaggacga gaaccgcccc
    29161 tggtacatgg cccgcgcctt cgcgcccgat gccgtgctga agatggcgct gcgcacccag
    29221 gccatcgcct tcccgcccga atcgcgcggc gtgcaggcca ttgccgacac gctgtcgcgc
    29281 cgcttcggcc agacctacga caacgtctac accttctacc tgtcgcgtcc cgcggacggc
    29341 gccgtgctcg acgaatacag ctgcgactgg atcgtggcca tgaccgagaa ggccaccggc
    29401 caggcgcgcg tgggctgcgg ccgctacgac tggactttcc gccagtcgcc gcaccaggcc
    29461 gagcggctgg tcatcaccat cgagaccatg ctcaccctgc cggccagcca ggccgacgcc
    29521 gtcctcgact ggatcacccg cctgccctat ccgtggaccg atgcgcagcg catcgtcgct
    29581 accgctcccg ccatcgaagc cctggcgccc gtgctgcaat ggatacggcg cgacgcaccg
    29641 catacgctga ccgcctgaac ccgtcccctc cccgccatcc ggaaacggcc atgcccaccc
    29701 aagacccgca acgcatcacc gacctggatg cgctgaccgc gctgtacggc gaaccgagca
    29761 cggcctccct ggtcaaggag atggaccacg tccacccgca ctaccgggcc ttcatcgaga
    29821 cggcgccctt cgcggcgctg gccacctgct cgcccggggg cctggacgtt tcgccgcgcg
    29881 gggacccggc cggcttcgtg cacatcgaag acgaaaaaac gctgatgatc cccgaccggc
    29941 gcggcaacaa ccgcgtcgac agcctgcgca acatcctggc cgacccgcgc gtggccctgc
    30001 tgttcctggt gcccggcgtg ggcgagaccc tgcgcgtgaa cggccgcgcc gagatccgcg
    30061 tggagcccgc cctgctcgag cgcttcgcca tggacggcaa gccgccgcgc tcggtgctcg
    30121 tcatccatgt ggaaaccgtg ttcttccagt gctcgcgcgc cgtggtgcgc gccggcctgt
    30181 gggacgccgg caggcagatc gcgcgcggcc ggttgcccag cacgggcagc atgctgtcgg
    30241 acttcagcaa gggaacgttc gacggcgcgg cctacgaccg cgacctggat gcgcgggtgc
    30301 aaagcacgct gtattgaaat cgaaagcggc agcctcgcgc agcgggactg cgtggggcgc
    30361 cagcggcgtc aggccggctg ccagccgcgc acgaacacat ccaccggctg gcgcttgccg
    30421 ccggccttct gcaactccag caggcgcagc acgccctcgc ccgtggcgat gtcgatgccc
    30481 cgctccgtgg cgtccagcac gctgccgggc gcgccggcgc cgcccggcag ggcctgcgcc
    30541 ttccacactt tcaccggctc ggccaggccc ggcaggcgca gcgtcgcgcc cggcaccggg
    30601 ttgaaggcgc gcacgcggcg ggccagcagt ggcgcgggct gcgtgcaatc gagcgcggcc
    30661 tcggccttgt cgagcttggc ggcgtaggtc gcgccggctt ccggctggcg gcgcggcgcg
    30721 agcgccggca aggcggccag ggcctcgacg atggcacggc cgcctgcatc ggccagcgcg
    30781 tcgtgcagtt ccgccgccgt ctgctgcgca ccgatcggca ccacgcgctc gagcagcatg
    30841 tcgcccgtgt ccaggccctc gtccatctgc atgatggtca cgcccgtctc ggcatcgccg
    30901 gcctcgatgg cgcgctggat gggcgccgcg ccgcgccagc gcggcaagag gctggcgtgg
    30961 atattcaggc agcccaggcg cggcagccgc agcgtccacg cgggcaggat gaggccgtag
    31021 gcggccacca ccatcacgtc cggcgcgatg cgttccagca ggtcgcgcgc ggccccggcc
    31081 tcgtcgggat acttgccgtc cagccgcagg ctgcgcggct gcgccacctc gatgcccgcc
    31141 tccagcgcgg cctgcttgac cgggctgggc gtgagcttga gcccgcggcc cgcggggcgg
    31201 tcgggctgcg tgagcacgag cgggatgtcg tgcccggcgg ccagcaaggc ctgcagggcg
    31261 atacgggaaa aatcgggggt tccggcgaag acgaggcgca tgggtcgagg acaaaagccc
    31321 aaaagcgcta ttgtccctca tcgcggcgcg cgaggatcac gaccgtcttc gcctcggcgc
    31381 tccggaaagg cggcggggcc cgaggagcga ctttccccgg tctcagcggc gggccgagag
    31441 acggcagcga tgtgaaccgt caagccagcc tgatgcgggg caatgtggtc ggccagcgca
    31501 tggcggtccg ggtaatcgcc gccattctgt ggacggaaag ctatgggctc gtcatcgaac
    31561 agccgctcca ctcggccgcg ccaggccgcg gctgctgcgg ccatgccttg ggcgtcgatt
    31621 ccgccagtcc catacaccgc gaaggtaggc agcacctgca tgccaggaaa gaacagcgtg
    31681 ccgtgcgtaa tgggaaagag cagttgctcc aacggcccgt tgatgccacg gggggaatag
    31741 tccttcgccg gcccaccaac ggagactgcc aatagcgcgc gcttgcccgc a
//
LOCUS       DASWPO010000043        22973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1795245, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000043 DASWPO010000000
VERSION     DASWPO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 22973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22973
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795245"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VGC69_02585"
     CDS             <1..635
                     /locus_tag="VGC69_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294204.1"
                     /translation="DDYAAFLLPPVLARFGEAHPLVTVEMICEPSVQLVRAVDEGRLD
                     LAITMRVPDRSMEFLRRERLVWVASPHHVPWQNDPLPIALFAPGCIVRTEVLRALGQA
                     DRAYRCTYSSPSLLGLLAVVQAGLAVTGLALCSVPSNLRIIGEAEGLPTLADAEVGIF
                     RSPGAMDSAVKCLDGFLRRELTVERDRSLAAEASVPLSSLESARLPSGLN"
     gene            723..1010
                     /locus_tag="VGC69_02590"
     CDS             723..1010
                     /locus_tag="VGC69_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0294205.1"
                     /translation="MYICICNAVTERQVRSCIDAGAASLDELQFELGVATGCGRCAGT
                     ACEYLPGGRASSVCSVRDITMPVNPPAMLAEHGHSQGQAANGFPVPIVVAA"
     gene            1115..2026
                     /locus_tag="VGC69_02595"
     CDS             1115..2026
                     /locus_tag="VGC69_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0294206.1"
                     /translation="MKSASLVSPSSAAPGGQRTAWGQIALFCILWSSAFASAKIALHD
                     CPPLILLAGRFLVAGLLMLGMAALTGGLRRISWRDFWCLVVLGVFNNGLYLGLSWSGM
                     TTVSSAFAAVLISTNPLLTGLLAGPVLGERLGWRQAAGLCLGLAGVVLVLRSRLTGMH
                     EDLHGTLLVAGGLVSLVAGTLVYKRLAPRSGLWITVGVQSLAGAVAVLPFALHYESLQ
                     HVRLTASLFWSMAYTIVAVSMGGYALWVTILKRQSATSASALHFLMPPLGLLFGWLAL
                     GEPVSWQDMAGILPIGLGIWLATRRAP"
     gene            complement(2035..3018)
                     /locus_tag="VGC69_02600"
     CDS             complement(2035..3018)
                     /locus_tag="VGC69_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0294207.1"
                     /translation="MKPPQTAPLLEVRDLVKHYPGQPDWLGRRRRAIQAVDGVSFTLA
                     RGETLSLVGESGCGKTTTGKSILRLIEPTSGSVRLDGVEITGLDAERMRLYRRDMQII
                     FQDPYASLNPRMTAGAIVAEPLRNFAQGLSAAQREERIAWLFSKVGLRPEATKLFPHE
                     FSGGQRQRLGIARALALNPKLIVCDEPVSALDVSVQAQVINLLNDLQAEFGIAYLFVA
                     HDLAVVRHISHRVAVMYLGQVVELADRGTLFAAPLHPYTQTLLSAAPLPNPHAKVRRL
                     LLRGDPPSPANPPSGCRFHTRCPLAQPRCAQEKPELTPRENGTQWVACHFR"
     gene            complement(3015..4061)
                     /locus_tag="VGC69_02605"
     CDS             complement(3015..4061)
                     /locus_tag="VGC69_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294208.1"
                     /translation="MTTLPNTTAVAPGDVLLEVDQLSTHFDTMAGTVKSVNGVSYTVR
                     AGKTLGVVGESGCGKSVTALSIMRLLPMPPARFAGGAIRYRGADLLALTEKQMRAIRG
                     NRISMIFQEPMTSLNPVLTVGRQIAETVMLHQKANRRVALERATEMLRLVQIAEPQRR
                     VAEYPHEMSGGMRQRVMIALALACNPEVLIADEPTTALDVTIQAQILELLGELQARLG
                     MGIVMITHDLGVVAETCERVVVMYAGSKVEEASVAELFDRPLHPYTRALMASMPSMNT
                     AERLTEIPGMVPAPSELGTGCSFAPRCAYAAERCRRETPPLAPQASDSGADHIVACFE
                     AERMAMLPPPGVRA"
     gene            complement(4084..4965)
                     /locus_tag="VGC69_02610"
     CDS             complement(4084..4965)
                     /locus_tag="VGC69_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294209.1"
                     /translation="MSGTTLSAPTSAPSSAALRWRRLRKHPTLILGVALLVVMAVIAL
                     AAPWLATHSPTAISPLTRMKPPSAQHYFGTDALGRDVYSRVVWGGRVSLTVAIAVAVL
                     STAIGVLLGLIAGFVRWADSIIMRIMDGLMAIPDILLAIALMAVIRASMTTVIVAIAI
                     PQVPRVVRLVRSLALTLREQLYVEAARAVGTRLPAILARHVLPNIVTPLVVQATFIAA
                     TAVLTEAVLSFLGVGVPPQVPSWGNMMAEGRNFVAVAFHIILFPGIFLAMTVLAINLT
                     GDGLRDALDPRLANRLK"
     gene            complement(4980..5915)
                     /locus_tag="VGC69_02615"
     CDS             complement(4980..5915)
                     /locus_tag="VGC69_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294210.1"
                     /translation="MAYVIRRLLATVPVMLVVAVVVFLLIHLSPGDPAALIAGDLASA
                     DDIARLHAALGLDEPLWRQFLLWAGKILHGNLGTSLFTQVPVTQLLAQRLGPTVSIAV
                     LTMLLSIVVAIPLGVLAAHRAGSWVDRLVMVFAVLAFSVPVFLVGYLFIYGFAVTLHW
                     LPVQGYASLSQGAWPWLRSLILPCVNLALVYIALITRMTRATVLEVLHEDYIRTARAK
                     GLGVLPVLGHALRNAAIPIATTVGVSIALLIGGVVVTETVFAIPGIGQLVIDSVQHHD
                     YPVIQSVLLLSAGVYVLINLLIDLSYKLFDPRIRY"
     gene            complement(5923..7488)
                     /locus_tag="VGC69_02620"
     CDS             complement(5923..7488)
                     /locus_tag="VGC69_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294211.1"
                     /translation="MKSTCLLALAAACLAAAPAVHAEGKTLRMVPYADVKVLDPFFTT
                     AYITRDFGFMVYDTLFAPDAKGVPQPQMVSRYTTSADGKLWTFTLRDGLKFSDGAPVK
                     AADCVASLQRWARHDNIGRAMTAAGGQWAAVDDKTFTLTLAQPFGLVLDGLAKLASYP
                     AFILPERLAKAPENAPIAEVVGSGPYIFKRDEWVPGNKVVFVRNPDYVGRSEPPSGLA
                     GNKASHVDRVEWTILPDANSAIAALKNGEVDMIEEIPPDYADTLNSDPDLRVGTSGNT
                     QAYLIMNTLFPPFNDPKARQAVLHAIDQDKFTLAMGYPDSMRTKYCATFFICGSPNDT
                     GAGAAPYAKPDLAQARQLLAASGYKGGKVAVLLPTDVSYLNAATLVAVQMLQDMGMDV
                     DVQSMDWATETARRANKNPPDQGGWSIYLSSAAEYSVNSPINSTYLGAACGNSLPGWP
                     CDAKLDQLRTAWIAATDPAQRKSLLDQFQERAYEAVPYAPIGQYARAYAVRKSLAHSE
                     LLWGLPNVWVLDK"
     gene            complement(7488..8927)
                     /locus_tag="VGC69_02625"
     CDS             complement(7488..8927)
                     /locus_tag="VGC69_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEY0294212.1"
                     /translation="MRVFCGGLGTETNTFSPMPTSLASFRDRGYFPAGTHPEGPTAQG
                     APLWVARQRARTDGWQVIEGPVASAQPSGTTTRLAYETLRDELLADLRAALPVDMVLL
                     GLHGAMVADGYDDCEGDLLTRVREIVGPGTVVGAELDPHAHLSPTIVAQTDVTVIYKE
                     YPHTDIVHCAEQLADLCLAKAQGRTRPVAALVDCEMIVPMHTTRDPAKGFVDRLRAME
                     GKDGILSLSYAQGFATGDVPDMGTKVLVYADGDEARAQRLARQLADELIGLREAMMVK
                     YRGVDEALDEALAFEGGPVVLADRADNPGSGAPGDSTYLLRRLLERKIGNAALGPLWD
                     PVAVRIAFDAGVGATLALRIGGKIGPLSGDPVDAVCTVEALEADMQMTSLTGTPTSMG
                     DCALVHTQGVDIVLTSVRNQAIDTDLFTQLGCDLAARKIVVVKSAQHFHASYAKVARH
                     ILYVGAPGVATPDWKTLPYKKIKRPKWPL"
     gene            9112..9588
                     /gene="bfr"
                     /locus_tag="VGC69_02630"
     CDS             9112..9588
                     /gene="bfr"
                     /locus_tag="VGC69_02630"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY0294213.1"
                     /translation="MQGDKQVIQYLNKQLTNELTAINQYYLHARMLDHWGFEKMGKHE
                     REESIEEMRHADKLIQRVFMLDGLPNLQDLHKLLIGEDVPEVLACDLKVEELSQATLK
                     EAIAYCESVRDFVSRDLMQEILDDTEDHIDYLETQIELVGKVGLQNYLQTQVHAEE"
     gene            complement(9658..10350)
                     /locus_tag="VGC69_02635"
     CDS             complement(9658..10350)
                     /locus_tag="VGC69_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294214.1"
                     /translation="MSWRFLFAVLVLAAGASAWGGIQLGDWMIGHAPQASANATVNQG
                     SAWSDQPVLDANGRPYVAQPPQPRVDGSLGVPDKPPAQDWSSRPPSLFDDTAPGVQVS
                     RTPVTPDQALAAADLNNAGLPAGASDVTTLDLGATPALPPSNGPLPPLVNGNISHAPL
                     IAQAPPLTVPPAQQAASGNWQTALHREVEQCSSQSFFQRPTCIWAARNKYCAPNHAWG
                     SVDDCPSRPGGE"
     gene            complement(10384..11991)
                     /locus_tag="VGC69_02640"
     CDS             complement(10384..11991)
                     /locus_tag="VGC69_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY0294215.1"
                     /translation="MSIPQEVARRRTFAIISHPDAGKTTLTEKLLLFAGAIQIAGSVK
                     ARKASRHASSDWMEIEKQRGISVASSVMQMEYRDCVINLLDTPGHQDFSEDTYRVLTA
                     VDAALMVIDAANGVEPQTIRLLQVCRARNTPIITFINKLDREVREPLDLLAEIESHLG
                     MDAVPFSWPIGMGKSFGGVFDLRRDRMRVFRPGQERLSDDDDIIEGLDNPEIAAKYGD
                     AFAKARDEIGLITDASPAFDRGAFLAGRQSPILFGSAINNFGVQEVLDTLVEQAPPPG
                     SRAAMERTVQPDEPKFTGVVFKVQANMDPAHRDRVAFLRVCSGQFERGMRMKVARTGK
                     EIKPNNVVTFMSQRRELLDDAYAGDIIGIPNHGILQLGDVLTEGESLRFTGLPFFAPE
                     LFQAVEVKDPLRTKQLRTGLTQLGEEGAIQVFRPEVSGGALLLGAVGQLQFEVVAHRL
                     KTEYGVDARMMPSRYTMARWITSDSPAALRKFMDANAAHIAYDVVDAAAFLISSPAQL
                     RVAEELYPNVKFHAMREHGGKVFSEKA"
     gene            complement(12142..13557)
                     /locus_tag="VGC69_02645"
     CDS             complement(12142..13557)
                     /locus_tag="VGC69_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0294216.1"
                     /translation="MRAIAETGYTTPTPIQAQAIPLVLQGGDLMAAAQTGTGKTAGFT
                     LPILQILGQNKPQQRKPGRPRCLILTPTRELTAQVAESVQTYGKYAGMTSMVIFGGVN
                     INPQISALKKPVDIMVATPGRLLDHAGQKTVDLSGVEILVLDEADRMLDMGFIHDIRR
                     VLRLLPAQRQNLLFSATFSEEIRTLARGILRDPGEVSVTPRNTTTESVAQSVLLTSQS
                     HKRDLVSHLIRESGWHQVLVFTRTKHGANRLAEKLVKDGLTAAAIHGNKSQSARTRAL
                     SGFKDGSVAVLVATDIAARGLDIDQLPLVVNFELPNVPEDYVHRIGRTGRAGATGSAI
                     SLVDDSEVKLLKAIERLINKPIERKPLPAGWTPPVRTAAEERAELDDRDDDERPRGGR
                     GQRGQGQGQRAGGNAGGNRGAKPAQASGRPAAAGPQGDRRSAGQQPARRPEGSRPQTA
                     GQGRNGAPRRDGAPRGALLGK"
     gene            13870..14487
                     /locus_tag="VGC69_02650"
     CDS             13870..14487
                     /locus_tag="VGC69_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0294217.1"
                     /translation="MNFARSLGAALAVLMLGLLSGCGYNGIQAADEQVKAAWSEVLNQ
                     YQRRADLVPQLVKTVDAYMTHEREVLTQVTEARSKVGQIQLTADALNDPQALQRFQQA
                     QAQLGSALSRLIAVSENYPQLKADGPFRDLQAQLEGTENRIAVARGRYVQAVQAYNLQ
                     VRQFPAVITAKIFGYAPKPNFSVENEAEISKAPQIQFSNAPKPAQ"
     gene            14465..15334
                     /locus_tag="VGC69_02655"
     CDS             14465..15334
                     /locus_tag="VGC69_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEY0294218.1"
                     /translation="MRPSPLSKGRLGQFAAVCALGLALLGFAPGWAGAQQAAPVPQLR
                     ARVTDQTGTLDAAYAQQLEQRLAALEQRKGAQLVVLIVGSTAPDTIEQYATRVFDQWK
                     LGRKKVDDGVLLVVAKNDRTLRIEVGYGLEGAVSDALASRIVREQIVPHFKSDDYAGG
                     IDAGVAALTKLIDGEPLPPPVAAPEQGVDNATNWGGALIGLLVLSIMPAPITGLLAGV
                     FIGLFTGSILWGLLAAVLGFALSFVLGVTGLKRGLMRGGGGFGGGGGFGGGGGGFGGG
                     GGRSGGGGASGRW"
     gene            complement(15350..16573)
                     /locus_tag="VGC69_02660"
     CDS             complement(15350..16573)
                     /locus_tag="VGC69_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0294219.1"
                     /translation="MSTDSILFTGGRVFDGLGKVLDGHAVLVEGGLIARVAPAGEFHG
                     YAGRKADTAGMTLMPGLGDCHVHLVYTGGADPNGIMGKQSDAEITLTALENAQTAIKG
                     GITAVRDCGGKDYLEFAVRNAIARGVFMGPTIRAAGRMICMTGGHGNRIGRVADGLDD
                     VVRAVREQVHAGCDLVKIMATGGVMTPGVNPMDAHYSAEEMRVGLHEAKRFHKSTASH
                     AQGTEGILNAVRGGVDSIEHGIYMDDECLSEMLERQTYLVPTIAALHNILHNADKGIP
                     SYVVEKARNVAERHRASIKMYYKAGGRIAMGTDAGTPYNLHGENAVELAYMVEFGMTP
                     VDALVAGTSRAHDLMGLVNAGRVLQGAQADLLLVQGDPTQDIACAAQRGNHVAVIKAG
                     VLAAGHLPGGAATGA"
     gene            complement(16570..18303)
                     /locus_tag="VGC69_02665"
     CDS             complement(16570..18303)
                     /locus_tag="VGC69_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294220.1"
                     /translation="MALLDIEDIRIEFPSRRGTRTAVDGITLALNKGEILGVVGESGA
                     GKSTIGNAVIGLLEAPGRLAGGQILLEGKRIDTLSDSEKRKVRGRRIGMIFQDPLTSL
                     DPLQTVENQLVETMQVHLDLTHAQAQQRVVALLTQVGIHQPELRAKQYPHQFSGGMRQ
                     RVVIALALCCEPEVIIADEPTTALDVSIQAQILDLLRKLCKEKQVGMIIITHDMGVIA
                     DVTDRVAVLYRGKLVEQGPTTKILGNPDHPYTRSLISAVPRPDVKLRRFPLVTYIEDV
                     KSPSAQIDVATHWLGKVRQDIVRKTGQGPLVQVHDLSLQFVLRNAFLKRNRRTLDAVK
                     HVSLDIPEGEVFGLVGESGSGKSTMARLISGLYTPTSGSVQFDGANLNLLRGERALNP
                     IRRQIQMVFQDPYSSVNPRMKVLDIIAEPIRFHKIAASEAEVRRIVADLLDVVGLGAQ
                     AAGRFPHEFSGGQRQRIAIARALATRPRFLICDEPTSALDVSIQAQILNLLKDLQEQL
                     HLTMLFISHDLPVIRQMCDRVGVMRHGELLEVAETEALFEHPQHEYTQHLLGLMPRLQ
                     LMSQEGLEIAQ"
     gene            complement(18314..19240)
                     /locus_tag="VGC69_02670"
     CDS             complement(18314..19240)
                     /locus_tag="VGC69_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294221.1"
                     /translation="MTSRIKTFFAAVADNDVWYSFWRTPTAIVAAIITLAILLGALLA
                     PVVSPHDPFDLASLNIMDANTPPSWMDGGSPDFLLGTDDQGRDILSAIFYGSRVSLVV
                     GFASVLFSLVLGVALGLVSGYAGGRVDSLIMRIADVQLSFPAILIALLVDGVARGILP
                     RDRQESMALYVLIFSIGISGWVQYARTVRSSTLVERNKEYVQAAKLIGIGPVTILRRH
                     ILPNVMGPVMVIATIHLAVAIITEATLSFLGVGVPPTAPSLGTLIRIGNSYLFSGMWW
                     ISIFPGVALIALVLAVNLLGDWLRDALNPKLR"
     gene            complement(19257..20228)
                     /locus_tag="VGC69_02675"
     CDS             complement(19257..20228)
                     /locus_tag="VGC69_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294222.1"
                     /translation="MLSFIARRLLQSLLVMVVVALIAFSMFRYVGDPIVAMVGQDTTE
                     QEREQLRHDLGLDDSFVVQFARFVAHAAVGDFGISYQQRRPVSELLAERFPATFELSL
                     VSAVMSIVLGIPMGIYTALRRRGVISKAFMALSLIGISLPTFLVGMLLILFFGVELRW
                     LPSFGRGDVVTLGGWTTGLLTGSGLKSVIMPAITLALFQMTLIMRLVRAEMLEVLRND
                     FIKFARARGLPERLINLRHALKNTLVPVITIIGLQLGGIIAFSIITETVFQWPGMGLL
                     FVQAIGSVDIPVMSAYLVLVAFIFVVINLVVDLLYFAVDPRLRVQSK"
     gene            complement(20380..21978)
                     /locus_tag="VGC69_02680"
     CDS             complement(20380..21978)
                     /locus_tag="VGC69_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294223.1"
                     /translation="MRIRSALKLSALTLALGASAYGACANARTLHWTYQGDASSMDPM
                     ALNETFTLGFQGNMYETLFGYDKDLKLVPVLAESWTNPEPNKWVIKLRQGVKFHDGSA
                     FTADDVIFSWQRSKTPGSDMKGYGALAADVKKIDDYTVEVDTPTPDPILPRDWSFLYM
                     MSKSWSEKNNTAQATNLKDAQGNYANMHENGTGPFMLVDRQPDVKTTLKRFPDYWDKG
                     IKTNIDEVVFQPIKEESTRVAALISGEMDIVQSVPVQDWERLQHTKGVKVLNRPEDRA
                     IFIGMDQKRDELLYSSVKGKNPFKDPKVRQAVVLAVDTAAINQKIMRGAAQPLGSLIA
                     TSINGYDPSFGAPIKPDPAQAKKLLAEAGYPKGFEVTLDCPNDRYVNDEKICQAVAAM
                     LTRVGIKIDLLAQTKGKYFAKVLPQGGNMTSMYLLGWSPSSVDADNALINLIHCPDGK
                     GMGQFNFGGYCNKDVDALEKQIGAETDQAKRNQLIKQAFTMVRNDWGYLPLHQPPLSW
                     GAKDTLQVIQRPDDVLDLRNVVMP"
     gene            22279..>22973
                     /gene="dctP"
                     /locus_tag="VGC69_02685"
     CDS             22279..>22973
                     /gene="dctP"
                     /locus_tag="VGC69_02685"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY0294224.1"
                     /translation="MKSRIAVAALALALAGAAGRSAAQAADTIELKVADSFPAGHYLV
                     ELMLKPWMADVTKRTNGKVKFSYFPNQQLGKATDLLNLTQSGVVDIGYIAPSYASDKM
                     PLSAVAELPGEFTRSCTGTMAYWKTARGGMLSKAEYQPNGIRLVMAVLLPPYKIFTAK
                     QKVETVQDIQGLKLRTTGGAQDITARTVNAVPVRMSAPDAYQALSRGTLDGLLFPDES
                     ISSYGLAKLVKHAT"
BASE COUNT         3534 a         7261 c         8376 g         3802 t
ORIGIN      
        1 cggacgacta tgcggctttc ctgctgccac ccgtgctggc gcgcttcggc gaggcgcacc
       61 cgctggtcac ggtcgagatg atctgcgagc cgtcggtgca attggtgcgc gcggtggacg
      121 agggccgcct ggatctcgcc atcacgatgc gcgtgccgga ccggtcgatg gaattcctgc
      181 gccgcgagcg tctggtctgg gttgcgtcgc cccaccacgt gccttggcag aatgacccgc
      241 tgcccatcgc gctgttcgcc ccgggctgca tcgtgcgtac cgaggtgctt cgggccctgg
      301 gtcaagccga cagggcctat cgctgtacct attcgagtcc cagcctgctc ggcctgctgg
      361 cggtggtgca ggcagggctg gccgtgacgg ggctggcgct gtgcagcgtg ccgtcgaact
      421 tgcgcatcat tggcgaggcc gagggcctgc cgacgctggc cgatgccgag gtcggcattt
      481 ttcggagtcc gggcgcgatg gactccgcgg tgaagtgcct ggatggcttt ttacgccgtg
      541 aactcacggt ggaacgagac agaagcctcg cagccgaggc tagcgtcccc ctgtcctccc
      601 ttgaaagcgc ccggcttccc tccggcctga actaggccag gtatttccag ggcgtttcca
      661 gttacaaatt tctgggcaac cccctcttgc gcatgcggat tttgttgttg ataataattc
      721 gcatgtatat ttgcatctgt aacgccgtta ccgaacgcca ggtccgctcc tgtatcgacg
      781 caggcgctgc gtcgctggac gagctgcagt tcgagctggg cgtggccacg ggctgcggcc
      841 ggtgcgccgg tacggcttgc gaatacctgc cgggcgggcg cgcttccagc gtgtgctcgg
      901 tgcgcgacat caccatgccg gtgaatccgc cggccatgct ggccgagcac ggccatagcc
      961 aaggccaggc ggccaacggc tttcctgtcc cgatcgtcgt cgcggcctga cccgcgtcgc
     1021 gtttcctccc cacgcgtttt cgtctcgccg ccaacggctg gtggcacaat gccgcaccgg
     1081 caggcatccc gctgccgcca cggaaacgcc tgtcttgaag tctgcttcgc tcgtctcgcc
     1141 ctcttccgcc gcaccgggcg gccaacgcac ggcatggggc cagatcgccc ttttctgcat
     1201 cctgtggagc tcggccttcg cctcggccaa gatcgccctg cacgattgcc cgcccctgat
     1261 cctgctggcc ggccgctttt tggtggcggg cctgctgatg ctgggcatgg cggcgctcac
     1321 gggcggcctg cggcgcatct cctggcgtga tttctggtgc ctggtggtgc tgggggtgtt
     1381 caacaacggc ctgtacctcg gcctgagctg gagcggcatg acgaccgtgt cgtcggcctt
     1441 cgcggcggtg ctgatcagca ccaatccgct cttgaccggc cttctggccg gccccgtgct
     1501 gggcgagcgc ctggggtggc gccaggccgc cgggctgtgc ctgggcctgg ccggcgtggt
     1561 gctggtgctg cgctcgcgcc tgacgggcat gcacgaagac ttgcacggca cgctgctggt
     1621 ggcgggcggc ctggtgagcc tggtggcggg cacgctggtc tacaagcgcc tggcgccgcg
     1681 gtccggccta tggatcaccg tgggcgtgca gtcgctggcc ggcgcggtgg cggtgctgcc
     1741 gtttgccctg cattacgaat cgctgcagca cgtgcgcctg acggccagcc tgttctggag
     1801 catggcctac accatcgtgg ccgtgtcgat gggcggctat gccctgtggg tcacgatcct
     1861 caagcgccag agcgccacgt cggccagcgc gctgcacttc ctgatgccgc cgctggggct
     1921 gctgttcggc tggctggcgc tgggcgagcc cgtgtcgtgg caggacatgg cgggcatcct
     1981 gccgatcggc ctgggcatct ggctggccac gcgccgcgcg ccctagcgcg ttctctaccg
     2041 gaaatggcag gcgacccact gcgtcccgtt ctcgcggggc gtcagctcgg gcttctcctg
     2101 ggcgcagcgc ggctgggcca gcggacagcg ggtgtggaag cggcagcctg aaggcgggtt
     2161 ggccgggctg ggcgggtcgc cgcgcagcag caggcgccgc accttggcgt gcgggttggg
     2221 caggggcgcg gccgacagca gggtctgcgt ataggggtgc agcggcgccg cgaacagcgt
     2281 gccgcggtcg gccagttcca ccacttgtcc caggtacatg accgccacgc ggtggctgat
     2341 gtggcgcacc acggccaggt cgtgcgccac gaacaggtag gcgatgccga actcggcctg
     2401 caggtcgttg agcaggttga tcacctgggc ctgcaccgag acgtccaggg ccgagacggg
     2461 ctcgtcgcag acgatgagct tggggttgag cgccagggcg cgcgcgatgc ccaggcgctg
     2521 gcgctggccg ccggagaatt cgtgggggaa gagcttcgtg gcctcggggc gcaggccgac
     2581 cttggagaac agccaggcga tgcgctcctc gcgttgcgcg gccgacagac cctgcgcgaa
     2641 gttgcgcaag ggttcggcca cgatggcgcc ggccgtcatg cgcgggttga gcgaggcgta
     2701 ggggtcctgg aagatgatct gcatgtcgcg gcggtacagc cgcatgcgtt cggcgtccag
     2761 gccggtgatc tcgacgccgt cgagccggac cgatcccgag gtgggttcga tcaggcgcag
     2821 gatgctcttg ccggtggtgg tcttgccgca gcccgattcg ccgaccaggg acagggtctc
     2881 gccgcgcgcc agcgtgaagg acaccccgtc gacggcctgg atggcgcggc gccgccggcc
     2941 cagccagtcg ggctggccgg ggtagtgctt gaccaggtcg cgcacctcga gcaggggggc
     3001 ggtctgcggc ggcttcatgc gcggactccc gggggcggca gcatggccat gcgttcggcc
     3061 tcgaagcagg ccacgatgtg gtcggcgccg ctgtccgagg cctggggggc gaggggcggc
     3121 gtttcgcgcc ggcagcgctc ggctgcgtag gcgcagcgcg gcgcgaagct gcagcccgtg
     3181 cccagctcgg acggcgcggg caccatgccc gggatctccg tcaggcgctc ggcggtgttc
     3241 atggacggca tcgaggccat cagggcgcgc gtgtaggggt gcagcgggcg gtcgaacagt
     3301 tccgccaccg aggcttcctc gaccttgctg ccggcgtaca tcaccaccac gcgctcgcag
     3361 gtctcggcga ccacgcccag gtcgtgggtg atcattacga tgcccatgcc gagccgggcc
     3421 tgcagctcgc ccagcagttc gaggatctgc gcctggatgg tcacgtccag cgcggtggtg
     3481 ggttcgtcgg cgatcagcac ctcggggttg cacgccagcg ccagcgcgat catgacgcgc
     3541 tggcgcatgc cgccggacat ttcgtgcggg tattcggcga cgcggcgctg cggctcggcg
     3601 atctgcacca ggcgcagcat ctcggtggcg cgttccaggg ctacacggcg gttggctttc
     3661 tggtgcagca tgacggtctc ggcgatctgc cggcccacgg tgagcaccgg gttcagcgag
     3721 gtcatgggct cctggaagat catcgagatg cggttgccgc ggatggcgcg catctgcttc
     3781 tcggtgagcg ccagcagatc ggcgccgcga taacggatcg cgccgccggc aaagcgcgcc
     3841 ggcggcatgg gcaagaggcg catgatggaa agcgccgtca cgctcttgcc gcagcccgac
     3901 tcgcccacca cgcccagcgt cttgccggcc cgcaccgtgt aggacacgcc gttgaccgac
     3961 ttgacggtgc cggccatcgt gtcgaagtgc gtggagagct ggtcgacctc gagcagcacg
     4021 tcgcccggcg cgacggcggt ggtgttgggc agcgtggtca taggcgtggg ggcttcgcgg
     4081 aggtcatttg agccggttgg ccagccgcgg gtcgagcgcg tcgcgcaggc cgtcgcccgt
     4141 caggttgatc gccagcacgg tcatggcgag gaagatgccg ggaaagagga tgatgtggaa
     4201 agccaccgcc acgaagttgc ggccttcggc catcatattg ccccagctgg gcacttgcgg
     4261 cggcacgccc acgccgagaa aggacagcac cgcctcggtg agcacggcgg tggccgcgat
     4321 gaaggtggcc tggaccacca gcggcgtgac gatgttgggc agcacgtggc gcgccaggat
     4381 ggccggcagg cgcgtgccga cggcgcgcgc ggcctcgacg tagagctgct cgcgcaaggt
     4441 cagcgccagc gagcgcacca gccgcaccac gcgcggcacc tgcgggatgg cgatggccac
     4501 gatgaccgtg gtcatgctgg cgcggatcac ggccatcagc gcgatggcca gcaggatgtc
     4561 gggtatcgcc atcaggccgt ccatgatgcg catgatgatc gagtcggccc agcgcacgaa
     4621 gccggcgatc aggcccagca gcacgcctat cgccgtggac aggaccgcca cggcgatggc
     4681 caccgtcagc gagacgcggc cgccccacac gacgcggctg tagacgtcgc ggcccagggc
     4741 gtcggtgccg aaatagtgct gcgcgctcgg cggcttcatg cgcgtcagcg ggctgatggc
     4801 cgtggggctg tgggtggcca gccagggcgc ggccagcgcg atgacggcca tcacgaccag
     4861 cagggcgacg cccaggatca gggtgggatg cttgcgcagg cggcgccagc gcagcgcggc
     4921 ggaagagggc gcggaagtcg gggcggatag cgtggtaccg gacatggctg cgctcgggct
     4981 cagtagcgga tgcgcggatc gaacagcttg tagctgaggt cgatcagcag attgatcagc
     5041 acgtagacgc ccgccgagag cagcagcacg ctctggatga cggggtagtc gtggtgctgc
     5101 accgagtcga tcaccagctg gccgatgccg ggaatggcga agacggtttc cgtcacgacc
     5161 acgccgccga tcaagagcgc gatgctcacg cccaccgtgg tggcgatggg gatggcggcg
     5221 ttgcgcaggg catgcccgag cacgggcagc acgcccaggc ccttggcgcg cgcggtgcgg
     5281 atgtagtctt cgtgcaggac ttccagcacg gtggcgcgcg tcatgcgcgt gatgagcgcg
     5341 atatagacca gcgccaggtt gacgcagggc agtatcaggc tgcgcaacca gggccacgcg
     5401 ccctgcgaca ggctggcgta gccttgcacc ggcagccagt gcagcgtgac cgcgaagccg
     5461 tagatgaaca ggtagcccac caggaacacc ggcaccgaga aggccagcac ggcgaacacc
     5521 atcaccagcc ggtccaccca ggagccggcg cggtgcgcgg ccagcacgcc cagcgggatg
     5581 gccaccacga tcgacagcag catggtcagc acggcgatgg agaccgtggg ccccagccgc
     5641 tgggccagca gctgcgtcac cggcacctgc gtgaagaggg aggtgccgag gttgccatgc
     5701 aggatcttgc cggcccacag caggaactgc cgccacagcg gctcgtccag gcccagcgcg
     5761 gcgtgcaggc gggcgatgtc gtcggcgctg gcgaggtcgc ccgcgatgag cgccgccggg
     5821 tcgccgggcg acaggtggat gagcaggaac accaccacgg ccaccaccag catcaccggc
     5881 acggtggcga gcaggcgccg gatcacatag gccatggagc gtctacttgt ccagcaccca
     5941 gacgttcggc aggccccaca gcagctcgct gtgcgccagg ctcttgcgca cggcgtaggc
     6001 gcgcgcgtac tggccgatgg gagcgtaggg aacggcctcg taggcacgct cctggaactg
     6061 gtcgagcagc gacttgcgct gggccgggtc ggtggccgcg atccacgcgg tgcgcagctg
     6121 gtcgagcttt gcgtcgcagg gccagcccgg caggctgttg ccgcaggccg cgcccaggta
     6181 ggtgctgttg atgggcgagt tgacgctgta ctcggcggcc gacgacaggt agatgctcca
     6241 gccgccctgg tcgggcgggt tcttgttggc gcggcgggcg gtctcggtgg cccagtccat
     6301 ggactgcacg tccacgtcca tgcccatgtc ctgcagcatc tgcaccgcca ccagcgtggc
     6361 cgcgttcagg taggacacgt cggtgggcag cagcaccgcg accttgccgc ccttgtagcc
     6421 ggacgcggcc agcagttgcc tggcctgcgc caggtcgggc ttggcgtagg gggccgcgcc
     6481 cgcgccggtg tcgttggggc tgccgcagat gaagaaggtg gcgcagtact tggtgcgcat
     6541 gctgtcgggg tagcccatgg ccagggtgaa cttgtcctgg tcgatcgcgt gcagcacggc
     6601 ctggcgcgcc ttcggatcgt tgaagggcgg gaacagcgtg ttcatgatca ggtaggcctg
     6661 ggtattgccc gaggtgccga cgcgcaggtc gggatcgctg ttcaaggtgt cggcgtagtc
     6721 gggcgggatt tcctcgatca tgtcgacttc gccgttcttc agcgcggcga tggcgctgtt
     6781 ggcatcgggc aggatggtcc attcgacgcg gtccacgtgc gaggccttgt tgccggccag
     6841 gcccgagggc ggctcgctgc ggccgacgta gtccgggttg cgcacgaaca ccaccttgtt
     6901 gcccggcacc cattcgtcgc gcttgaagat atatggtccg gagcccacca cctcggcgat
     6961 cggcgcgttc tccggcgcct tggccaggcg ctcgggcagg atgaaggcgg ggtagctggc
     7021 cagcttggcc aggccgtcga gcaccaggcc gaagggctgg gcgagcgtca gcgtgaaggt
     7081 cttgtcgtcg accgcggccc attgcccgcc ggccgccgtc atggcgcggc cgatattgtc
     7141 gtggcgggcc cagcgctgca gcgaggccac gcagtcggcc gccttcaccg gcgcgccgtc
     7201 gctgaacttc aggccgtcgc gcagggtgaa ggtccagagc ttgccgtcgg ccgaggtcgt
     7261 gtagcgcgac accatctgcg gctgcggcac gcccttggcg tcgggcgcga acaaggtgtc
     7321 gtacaccatg aagccgaagt cgcgggtgat gtaggccgtg gtgaagaacg ggtccagcac
     7381 cttgacgtcg gcgtagggga ccatgcgcag cgtcttgcct tcggcgtgga cggcgggcgc
     7441 ggcggccagg caggccgccg ccagggcgag caggcacgtg gatttcatca gagaggccat
     7501 ttgggtcgct tgatcttctt gtagggcagg gttttccagt cgggcgtggc gacgcccggc
     7561 gcgcccacgt agaggatgtg ccgggccacc ttggcatacg aggcatggaa atgctgggcc
     7621 gacttcacca ccacgatctt gcgggcggcc aggtcgcagc cgagctgggt gaagaggtcg
     7681 gtgtcgatgg cctggttgcg caccgaggtg agcacgatgt ccacgccctg cgtatgcacc
     7741 agcgcgcagt cgcccatgga cgtgggcgtg ccggtgaggc tggtcatctg catgtcggcc
     7801 tcgagcgcct cgacggtgca gaccgcgtcg accgggtcgc ccgacagcgg gccgatcttg
     7861 ccgccgatgc gcagcgccag cgtggcgccc acgcccgcgt cgaaggcgat gcgcacggcc
     7921 accgggtccc acagcgggcc cagggcggca ttgcctatct tgcgttcgag caggcggcgc
     7981 agcaggtagg tggaatcgcc cggcgcgccg ctgccggggt tgtcggcgcg gtcggccagc
     8041 accaccgggc cgccctcgaa ggccagcgct tcgtcgaggg cctcgtccac gccgcggtac
     8101 ttgaccatca tggcctcgcg caggccgatg agctcgtcgg cgagctggcg ggccagccgc
     8161 tgcgcccgag cctcgtcgcc gtcggcgtag accagcacct tggtgcccat gtcgggcacg
     8221 tcgccggtgg cgaagccctg cgcgtaagac agcgacagga tgccgtcctt gccttccatg
     8281 gcgcgcaggc ggtcgacgaa gcccttggcc gggtcgcgcg tggtgtgcat gggcacgatc
     8341 atttcgcagt cgaccagcgc ggccacgggc cgggtcctgc cctgcgcctt ggcgaggcac
     8401 aggtcggcga gctgctcggc gcagtgcacg atgtcggtgt gcggatattc cttgtagatc
     8461 acggtgacgt cggtctgcgc cacgatggtc ggactcaggt gggcgtgcgg gtcgagttcg
     8521 gcgccgacca cggtgccggg accgacgatt tcgcgcacgc gggtaagcag gtcgccctcg
     8581 cagtcgtcgt agccgtcggc caccatggcg ccgtgcaggc ccagcagcac catgtccacc
     8641 ggcagcgcgg cgcgcaggtc ggccagcagt tcgtcgcgca aggtctcgta ggccaggcgc
     8701 gtggtggtgc cgctgggctg ggccgaggcg accggacctt cgatgacctg ccagccgtcg
     8761 gtcctggcgc gctggcgcgc cacccacagg ggggcgcctt gcgcggtggg gccttcggga
     8821 tgcgtgccgg cgggaaagta gccgcggtcg cggaaagaag ccaggctggt gggcatgggc
     8881 gaaaacgtat tggtttccgt tcccagtcca ccgcaaaaca ctcgcatgat cgatcctcga
     8941 taacccatgg tcagggggcg atgacaaggc gtcgagtatc tgcccgggcg gcacgcgatg
     9001 gcaatgggaa caaacgctag ggatgtgcaa gggccggcgc tgcgatctgt ccggcaggat
     9061 gtgttagcct gccgtcagtt gcggcgtaga ccgtcgaatc gggagtacat catgcagggc
     9121 gacaagcaag tcatccaata cctcaacaag cagctcacca acgagctcac ggccatcaac
     9181 cagtattacc tgcatgcccg catgctggac cactggggct tcgagaaaat gggcaagcac
     9241 gagcgcgagg aatccatcga agagatgcgc catgccgaca agctgatcca gcgcgtcttc
     9301 atgctcgacg gcctgcccaa cctgcaggac ctgcacaagc tgctgatcgg cgaggacgtg
     9361 cccgaagtgc tggcgtgcga cctcaaggtg gaagagctgt cgcaggccac gctgaaagag
     9421 gccatcgcct attgcgaatc ggtgcgcgat ttcgtgtcgc gcgacctgat gcaggaaatc
     9481 ctggacgaca ccgaagacca catcgactat ctggaaaccc agatcgaact ggtgggcaag
     9541 gtgggcctgc aaaactacct gcagacccag gtccacgcag aggaatgaag cctcgccgcc
     9601 atgaaaaaag gcgcctgcgt ggcgcccttt ttttcatggc ggcgacgacg gtcgccgtca
     9661 ctctcctccc ggtcgagagg ggcagtcgtc gaccgagccc caggcatgat tgggcgcgca
     9721 gtatttgttg cgggccgccc agatgcaggt ggggcgctgg aagaagctct ggctggagca
     9781 ctgttcgact tcgcgatgca gggccgtctg ccagttgccc gatgcggcct gttgcgccgg
     9841 cgggaccgtg aggggcgggg cctgggcgat caagggcgcg tggctgatat tgccgttgac
     9901 cagcggcggc agcgggccgt tggaaggcgg cagggccggg gtggcgccga ggtcgagggt
     9961 ggtcacgtcg gaggcgccgg cgggcaggcc ggcattgttg aggtcggccg cggccagggc
    10021 ctggtccggg gtgacggggg tgcgcgagac ctgcacgccg ggggcggtgt cgtcaaacag
    10081 cgacgggggg cggctggacc agtcctgcgc gggcggcttg tcgggcacgc ccagggagcc
    10141 gtcgacgcgg ggctgcggcg gctgggcgac gtagggcctg ccgttggcgt cgagcacggg
    10201 ctggtcggac caggcgctgc cctggttgac cgtggcgttg gcgctggcct gcggcgcgtg
    10261 gccgatcatc cagtcgccta gctggattcc gccccaggcg gaggcgccgg ccgccaaaac
    10321 gagtactgca aacagaaaac gccacgacat tgcttctagc ctcttttgcc cgccgatgcg
    10381 ccctcaggcc ttttcagaaa aaaccttgcc gccgtgctcc cgcatggcgt ggaatttgac
    10441 attcggatac aactcttcgg ccacccgcag ctgggctggc gagctgatca agaaggcggc
    10501 cgcatcgacc acatcatagg cgatatgggc ggcattggca tccatgaact tgcgcagcgc
    10561 ggcgggcgaa tcggaggtga tccagcgggc catggtgtag cgcgagggca tcatgcgggc
    10621 atccacgccg tactcggttt tcaggcggtg cgccaccacc tcgaattgca gctggccgac
    10681 cgcgcccagc agcagggcgc cgccggagac ctcgggacgg aacacctgga tggcgccctc
    10741 ttcgcccagc tgggtcaggc cggtgcgcag ctgcttggtg cgcagcggat ccttcacttc
    10801 cacggcctgg aacagctcgg gcgcgaagaa gggcaggccg gtaaagcgca ggctctcgcc
    10861 ttcggtgagc acgtcgccca gctgcaggat gccgtgattg ggaatgccga tgatgtcgcc
    10921 cgcgtaggcg tcgtcgagca gctcgcgccg ctgcgacatg aaggtcacca cgttgttggg
    10981 cttgatttcc ttgccggtgc gcgccacttt catgcgcatg ccgcgctcga actggccgga
    11041 gcacacgcgc aggaaggcca cgcggtcgcg gtgggccggg tccatattgg cctgcacctt
    11101 gaacaccacg ccggtgaact tgggctcgtc gggctgcacc gtgcgttcca tcgccgcgcg
    11161 cgagcccggc ggcggggcct gctcgaccag ggtgtccagc acttcctgca cgccgaagtt
    11221 gttgatggcc gagccgaaga gaatgggcga ctgcctgccg gcgaggaagg cgccgcggtc
    11281 gaaggcgggc gaggcgtcgg tgatcaggcc gatctcgtcg cgggccttgg cgaaggcgtc
    11341 gccgtacttg gcggcgattt ccgggttgtc gaggccctcg atgatgtcgt catcgtcgga
    11401 gaggcgttcc tggccggggc ggaagacgcg catgcggtcg cggcgcaggt cgaatacgcc
    11461 gccgaagctc ttgcccatgc cgatcggcca cgagaagggc acggcgtcca tgcccaggtg
    11521 cgattcgatc tcggccagca ggtcgagcgg ttcacgcact tcgcggtcga gcttgttgat
    11581 gaaggtgatg atgggcgtgt tgcgcgcgcg gcagacctgc agcaggcgga tggtctgcgg
    11641 ctccacgccg ttggccgcgt cgatcaccat cagcgccgcg tccaccgcgg tgagcacgcg
    11701 gtaggtgtct tccgagaagt cctggtggcc cggggtgtcg agcaggttga tgacgcagtc
    11761 gcggtattcc atctgcatca ccgacgaggc caccgaaatg ccgcgctgct tttcgatctc
    11821 catccagtcc gacgaggcgt ggcgcgaggc cttgcgggcc ttgacgctgc cggcgatctg
    11881 gatggcgcct gcgaacagca gcagcttttc cgtgagcgtg gtcttgcccg cgtcggggtg
    11941 ggagatgatg gcgaaggtgc gtcgccgggc gacttcctgg gggatgctca tggtgcgtat
    12001 acgggggacg gcaggttcag gcgtaccgac ggatccagag gaaaagcgtc atgctggagg
    12061 gcacgcgcaa agagggcagc gcgggcagcg cgggcagcgc gggcagcgcg gcccggccgt
    12121 cggacgaccg ggcctggcag tttacttgcc cagcaatgcg ccgcgcggcg cgccgtcgcg
    12181 gcggggagcg ccgttgcggc cctggcccgc ggtttgcggc cgcgagcctt ccgggcggcg
    12241 cgcgggctgc tggccggcgc tgcggcggtc gccttgcggc ccggccgcgg ccgggcggcc
    12301 gctggcctgg gccggcttgg cgccgcgatt gccgcctgcg ttgccgccgg cgcgctggcc
    12361 ttggccctgg ccgcgctggc cacggccgcc gcgcgggcgt tcgtcatcgt cgcggtcgtc
    12421 gagctcggcg cgctcttcgg cggccgtgcg cacgggcggc gtccagccgg cgggcagggg
    12481 cttgcgctcg atgggcttgt tgatgaggcg ttcgatggcc ttgagcagct tgacctcgct
    12541 gtcgtcgacc agcgaaatgg ccgagcccgt ggcgcccgcg cggccggtgc ggccgatgcg
    12601 gtgcacgtag tcttccggca cgttgggcag ttcgaaattg accaccagcg gcagctggtc
    12661 gatgtccagg ccgcgcgcgg cgatgtcggt ggccaccagc acggcgacgc tgccgtcctt
    12721 gaagccggac agggcgcggg tgcgcgccga ctggctcttg ttgccgtgga tggcggcggc
    12781 cgtcaggccg tccttgacca gtttttcggc caggcggttg gcgccgtgct tggtgcgggt
    12841 gaagaccagc acctggtgcc agccgctttc gcggataagg tggctgacca ggtcgcgctt
    12901 gtgcgactgc gaggtcagga gcaccgactg cgccacgctc tcggtggtgg tgttgcgcgg
    12961 ggtgaccgag acttcgcccg gatcgcgcag gatgccgcgc gcgagcgtgc ggatttcctc
    13021 ggagaacgtg gccgagaaca gcaggttctg gcgctgtgcg ggcaggagcc tgagcacccg
    13081 gcggatgtcg tggatgaagc ccatgtcgag catgcggtcg gcttcgtcca gcaccaggat
    13141 ctcgaccccg gagaggtcga cggtcttctg gccggcatgg tcgagcaggc ggccgggcgt
    13201 ggctaccatg atgtccacgg gctttttcag cgcgctgatc tgcgggttga tgttgacgcc
    13261 gccgaatatc accatggacg tcatgccggc gtacttgccg taggtctgca ccgactcggc
    13321 cacctgggcg gtgagttcgc gggtgggggt gaggatgagg cagcgcgggc ggccgggctt
    13381 gcgttgctgc ggcttgtttt gcccgaggat ctgcaggatg ggcagggtga agcccgccgt
    13441 cttgcccgtg ccggtctggg cggcggccat gaggtcgcct ccctggagga cgagcggaat
    13501 cgcctgggcc tggatgggcg tgggggtggt atagccggtt tcggcgatgg cacgcaaaag
    13561 cgtgtcggcc agaccgaggt cggcaaaggc attcgaggtg gtagtcaaga caactccagc
    13621 gtcaacccgt cgctctggtt tgagagacac caatccgggc tggcaaatga ggaggaaggg
    13681 gtagtcgcgg ctggctgcgg agcaggggca ctgcgacatc ccggcgcgtg gcggctaagc
    13741 gtcagcggga gcgtgccgat tgggtaggca cgcaagcatt gtagccgatc ggggcggctt
    13801 gcgtgggcaa gtaggcaagg cgctgacaag ctgctgtaat gcgtccattc ccccatcacg
    13861 gagcaagaca tgaatttcgc gcgtagtctg ggcgccgccc tggcggtgct gatgcttggc
    13921 ctgttgtctg gctgcggcta caacggcatc caggcggccg acgagcaggt caaggcggcc
    13981 tggtccgaag tgctcaacca gtaccagcgc cgcgccgacc tggtgccgca gttggtcaag
    14041 acggtcgacg cctacatgac gcacgagcgc gaggtgctca cgcaagtgac cgaggcgcgc
    14101 agcaaagtag ggcagatcca gctgacggcc gatgcgctga acgacccgca agccctgcag
    14161 cgcttccagc aggcccaggc ccagctgggg tcggccttgt ccaggctcat cgcggtgtcc
    14221 gagaactatc cgcagctcaa ggccgacggt ccgttccgcg acctgcaggc gcagctcgag
    14281 ggcaccgaga accgcatcgc cgtggcgcgg ggccgctatg tgcaggcggt gcaggcctac
    14341 aacctgcagg tgcgccagtt ccccgccgtg atcacggcca agatcttcgg ctatgcgccc
    14401 aagcccaatt tcagcgtcga gaacgaggcc gagatatcca aggcgccgca aatccagttc
    14461 agcaatgcgc ccaagcccgc tcagtaaagg tcggctcggc cagttcgcgg ccgtttgcgc
    14521 gctggggctg gcgctgctgg gtttcgcgcc cggctgggcc ggcgcacagc aagccgcgcc
    14581 cgtgccgcag ctcagggccc gcgtcaccga ccagaccggc acgctggacg ccgcctatgc
    14641 gcagcagctc gaacaacgcc tggcggcgct cgagcagcgc aaaggcgcgc agctcgtggt
    14701 gctcatcgtg ggcagcaccg cgcccgacac catcgagcaa tacgccaccc gcgtgttcga
    14761 ccagtggaag ctcggccgca agaaggtgga cgacggcgtg ctgctggtcg tggccaagaa
    14821 cgaccgcacc ctgcgcatcg aggtcggcta cgggctggaa ggcgcggttt ccgatgcgct
    14881 ggccagccgc atcgtccgcg agcagatcgt gccgcatttc aagtcggacg actacgccgg
    14941 cggcatcgac gccggcgtgg cggcgctgac gaaactgatc gacggcgagc ccctgccgcc
    15001 gcctgtggcc gcgccggagc agggcgtcga taacgcgacg aactggggcg gcgccctcat
    15061 cggcctgctg gtcctgtcga taatgcccgc gcccatcacc gggctgctgg ccggtgtttt
    15121 catcgggctg ttcacgggca gcatcctgtg ggggctgctg gccgcggtgc tgggctttgc
    15181 cctcagtttc gtgctgggcg tgacgggtct gaagcgcggc ctcatgcgtg gcggcggcgg
    15241 attcggcggc ggtggcgggt ttggcggggg cggtgggggg tttggcggcg ggggcggccg
    15301 cagcgggggc ggcggcgcgt cgggccgctg gtagccgccc gcccggcgct caggcgcccg
    15361 tggccgcacc gccgggaagg tggcccgctg ccagcacgcc tgccttgatg acggcgacgt
    15421 ggttgccgcg ctgggcggcg caggcgatgt cctgcgtggg gtcgccttgc accagcagca
    15481 ggtcggcctg ggcgccctgg aggacgcggc cggcgttgac caggcccatg aggtcgtgcg
    15541 cgcgggacgt gccggccacc agggcgtcga ccggggtcat gccgaactcg accatatagg
    15601 ccagctccac cgcgttctcg ccgtggaggt tgtagggcgt gccggcatcg gtgcccatgg
    15661 cgatgcgtcc gcccgccttg tagtacatct tgatcgacgc gcggtggcgc tcggccacgt
    15721 tgcgggcctt ttccaccacg taggacggga tgcccttgtc ggcgttgtgc aggatgttgt
    15781 gcagggccgc gatggtcggc accaggtagg tctggcgctc gagcatctcg ctcaggcact
    15841 cgtcgtccat gtagatgccg tgctcgatgg agtccacccc gccgcgcacg gcgttgagga
    15901 tgccctcggt gccctgcgcg tggctggccg tgctcttgtg gaaacgcttg gcctcgtgca
    15961 ggcccacgcg catctcttcg gcgctgtaat gcgcgtccat gggattgacg ccgggcgtca
    16021 tcacgccgcc ggtggccatg atcttgacca ggtcgcagcc ggcgtgcacc tgttcgcgca
    16081 ccgcgcggac cacgtcgtcg agcccgtcgg ccacgcggcc gatgcggttg ccgtggccgc
    16141 ccgtcatgca gatcatgcgg ccggcggcgc ggatggtggg gcccatgaag acgccgcggg
    16201 cgatggcgtt gcgcacggcg aattcgaggt agtccttgcc gccgcagtcg cgcacggcgg
    16261 tgatgccgcc cttgatcgcg gtctgggcgt tttccagcgc ggtcagcgtg atctcggcgt
    16321 cggactgctt gcccatgatg ccgttgggat cggccccgcc ggtatagacc aggtgcacgt
    16381 ggcagtcgcc caggccgggc atcagcgtca tgccggcggt gtcggccttg cggcccgcgt
    16441 agccgtggaa ctcgccggcc ggggcgacgc gggcgatcag cccgccttcg accagcacgg
    16501 cgtggccgtc caggaccttg cccaggccgt cgaagacgcg gccgccggta aagagtatcg
    16561 aatcggtgct cattgcgcga tctccagtcc ttcctgcgac atgagttgca ggcgcggcat
    16621 caggcccagc aggtgctggg tgtattcgtg ctgcgggtgt tcgaacagcg cctcggtctc
    16681 ggccacctcg agcagctcgc cgtggcgcat aacgcccacg cggtcgcaca tctggcggat
    16741 cacgggcagg tcgtggctga tgaagagcat ggtcaggtgg agctgttcct gcaggtcctt
    16801 gagcaggttg aggatctgcg cctggatgga tacgtccagc gccgaggtgg gctcgtcgca
    16861 gatcaggaag cgcggccgcg tggccagcgc gcgggcgatg gcgatgcgct ggcgctggcc
    16921 gcccgagaac tcgtgcggaa agcgcccggc cgcctgcgcg cccaggccca ccacgtcgag
    16981 caggtcggcc acgatgcggc gcacctcggc ctcgctggcg gcgatcttgt gaaagcggat
    17041 gggctcggcg atgatgtcca gcaccttcat gcgcggattg accgacgagt acgggtcctg
    17101 gaagaccatc tggatctggc ggcggatggg gttgagcgcc cgttcgcccc tgagcaggtt
    17161 gagattggcg ccgtcgaact gcaccgagcc gctggtgggc gtgtacaggc ccgagatcag
    17221 gcgcgccatg gtggacttgc ccgagcccga ctcgcccacc aggccgaaga cctcgccctc
    17281 ggggatgtcg aggctgacgt gcttgacggc atcgagcgtg cggcggttgc gcttgagaaa
    17341 ggcattgcgc agcacgaact gcaggctcag gtcgtgcacc tgcaccagcg ggccctggcc
    17401 ggtcttgcgg acgatgtcct ggcggacctt gcccagccag tgcgtggcca cgtcgatttg
    17461 cgccgacggc gacttcacgt cctcgatata ggtcaccagc gggaagcgcc gcagcttgac
    17521 gtcgggccgc ggcacggccg agatcaggct gcgggtgtag gggtggtcgg ggttgccgag
    17581 gatcttggtc gtggggccct gttccaccag cttgccgcgg tagagcaccg ccacgcggtc
    17641 ggtcacgtcg gcgatcacgc ccatgtcgtg cgtgatgatg atcatgccca cctgcttctc
    17701 tttgcacagc ttgcgcagca ggtcaaggat ctgcgcctgg atggacacgt ccagggcggt
    17761 ggtgggctcg tcggcgatga tgacctcggg ctcgcagcac agcgccaggg cgatcaccac
    17821 gcgctggcgc atgccgcccg agaactggtg cggatactgc ttggcgcgca gctcgggctg
    17881 gtggatgccc acctgggtga gcagcgccac cacgcgctgc tgcgcctggg cgtgggtgag
    17941 gtccaggtgc acttgcatgg tttcgaccag ctggttttcc accgtctgca gcgggtcgag
    18001 cgaggtgagc ggatcctgga agatcatgcc gatgcgccgg ccgcgcacct tgcgcttctc
    18061 tgaatcggac agggtgtcga tacgcttgcc ctcgagcagg atctggccgc cggccaggcg
    18121 cccgggggct tcgagcaggc cgatgacggc gttgccgatg gtggacttgc cggcgccgga
    18181 ttcgccgacc acgccgagga tctcgccctt gttcagcgcg agggtgatgc cgtcgaccgc
    18241 ggtgcgggtg ccgcgccggc tggggaattc gatgcggatg tcttcgatgt cgagcagggc
    18301 catggcggtt tcctcagcgc agcttgggat tgagggcgtc gcgcagccag tcgcccagca
    18361 ggttgaccgc cagaaccagg gcgatgagcg ccaccccggg aaagatcgag atccaccaca
    18421 tgccggagaa cagatagctg ttgccgatgc ggatcagcgt gcccagcgag ggcgcggtgg
    18481 gcggcacgcc cacccccagg aaggacagcg tggcctcggt gatgatggcg acggccaggt
    18541 ggatggtggc gatcaccatc acggggccca tcacattggg caggatgtgg cggcgcagga
    18601 tggtgaccgg gccgatgccg atgagcttgg ccgcctgcac gtactccttg ttgcgctcga
    18661 ccagggtgga gctgcgcacg gtgcgcgcgt actgcaccca gcccgagatg ccgatggaga
    18721 agatcagcac gtacagggcc atggattcct gccggtcgcg cggcaggatg ccgcgcgcca
    18781 cgccgtcgac cagcagcgcg atcaggatgg cgggaaacga cagctgcacg tcggcgatgc
    18841 gcatgatgag gctgtcgacg cgcccgccgg catagccgct caccaggccc agggccacgc
    18901 ccagcaccag cgagaacagc accgaggcga agcccaccac cagcgacacg cgcgagccgt
    18961 agaagatggc cgagaggatg tcgcggccct ggtcgtcggt gcccagcagg aagtccgggc
    19021 tgccgccgtc catccaggaa ggcggcgtgt tggcgtccat gatgttcagc gacgccaggt
    19081 cgaaggggtc gtgcggggac accacggggg ccagcagggc gcccagcagg atggccagcg
    19141 tgatgatggc cgcgacgatg gcggtgggcg tgcgccagaa gctgtaccag acgtcgttgt
    19201 cggccacggc ggcgaagaag gtcttgatgc gggaagtcat tgcgcgtcct cgaatatcac
    19261 ttgctttgca cgcgcagtcg gggatcgacg gcaaagtaca gcagatcaac caccaggttg
    19321 atcaccacga agatgaaggc caccagcacc aggtaggccg acatcacggg gatgtcgacc
    19381 gagccgatgg cctggacgaa caacaggccc atgccgggcc actggaacac ggtctcggta
    19441 atgatggaga aggcgatgat gccgcccagc tgcaggccga tgatggtgat gaccggcacc
    19501 agcgtgttct tgagcgcgtg gcgcaggttg atgagccgct cgggcaggcc gcgcgcgcgg
    19561 gcgaacttga tgaagtcgtt gcgcagcacc tcgagcatct cggcgcgcac caggcgcatg
    19621 atgagcgtca tctggaacag ggccagcgtg atggccggca tgatgaccga cttcaggccg
    19681 gacccggtga gcagcccggt ggtccagccg cccagcgtca ccacgtcgcc gcgcccgaag
    19741 ctgggcagcc agcgcagctc cacgccgaag aacaggatga gcagcatgcc gaccaggaag
    19801 gtcggcagcg aaatgccgat cagcgacagc gccatgaacg ccttggagat cacgccgcgg
    19861 cgccgcagcg ccgtgtagat gcccatgggg atgccgagca cgatggacat cacggccgag
    19921 acgagggaaa gctcgaacgt ggccgggaac cgctcggcca gcagctcgct gacgggacgg
    19981 cgctgctggt aggagatgcc gaaatcgccc acggcggcgt gggcgacgaa acgggcgaac
    20041 tggacgacga aggaatcgtc gagcccgagg tcgtggcgca attgctcgcg ctcttgctcg
    20101 gtggtgtcct gccccaccat ggcgacgatg gggtcgccca cgtagcggaa cattgaaaag
    20161 gcgatcagtg cgaccaccac catgaccagc agcgactgca aaagtcgtcg cgcgataaag
    20221 gaaagcatgt gcgggtgccc tcggatccag ctgttgctgc ggccggatct tggttggtgt
    20281 gagtgcggag cagggtttgc cgcgggtaat gcccgggctg cccgagcggg ccgcccgcca
    20341 tggccggagc ggcccgttcg aaccgctccg gggactgcgt caaggcatca cgacgttgcg
    20401 caggtcgagc acgtcatcgg gacgctggat cacctggagc gtgtccttcg cgccccagga
    20461 cagcggcggc tggtgcaggg gcaggtagcc ccagtcgttg cgcaccatcg tgaaggcttg
    20521 cttgatcagc tggttgcgct tggcctggtc ggtctcggcg ccgatctgct tttcaagcgc
    20581 gtcgacgtcc ttgttgcagt agccgccgaa gttgaactgg cccatgccct tgccgtcggg
    20641 gcagtggatc aggttgatca gcgcattgtc ggcgtcgacc gagctgggcg accagcccag
    20701 caggtacatg ctggtcatgt tgccgccctg cggcagcacc ttggcgaagt acttgccctt
    20761 ggtctgggcc agcaggtcga tcttgatgcc gacgcgggtc agcatggcgg cgacggcctg
    20821 gcagatcttc tcgtcgttga cgtagcggtc gttggggcag tccagcgtca cctcgaagcc
    20881 cttgggatag ccggcctcgg ccagcagctt cttggcctgc gcggggtcgg gcttgatcgg
    20941 ggcgccgaac gaggggtcgt agccgttgat cgaggtggcg atcagcgagc ccagcggctg
    21001 cgccgcgccg cgcatgatct tctggttgat ggcggcggtg tccaccgcca gcaccacggc
    21061 ctggcgcacc ttcgggtcct tgaaggggtt cttgcccttg acgctggagt acagcagctc
    21121 gtcgcgcttc tggtccatgc cgatgaagat ggcgcggtct tcgggccggt tgagcacctt
    21181 cacgcccttg gtgtgctgca ggcgctccca gtcctgcacc ggcacggact ggacgatgtc
    21241 catctcgccc gagatcagcg cggccacgcg ggtcgattct tccttgatgg gctggaacac
    21301 gacctcgtcg atattggtct tgatgccctt gtcccagtag tcggggaagc gcttgagcgt
    21361 ggtcttcacg tcgggctggc ggtcgaccag catgaagggg cccgtgccgt tctcgtgcat
    21421 gttggcgtag ttgccctggg cgtccttcag gttggtggcc tgggcggtgt tgttcttctc
    21481 ggaccacgac ttgctcatca tgtagaggaa ggaccagtcg cgcggcagga tggggtcggg
    21541 cgtgggcgtg tcgacctcga cggtgtagtc gtcgatcttc ttcacgtcgg cggccagcgc
    21601 gccgtagccc ttcatgtcgg agccgggggt cttgctgcgc tgccacgaga agatcacgtc
    21661 gtcggcggtg aaggccgagc cgtcgtggaa cttgacgccc tggcgcagct tgatcaccca
    21721 cttgttgggt tcggggttgg tccagctttc ggccaggacg ggcacgagct tgagatcttt
    21781 gtcgtagccg aacagggttt cgtacatatt gccctggaag cccagcgtga aggtctcgtt
    21841 cagcgccatc ggatccatcg aggatgcgtc gccctggtag gtccagtgca gggtgcgggc
    21901 gttggcgcag gcgccgtagg ccgaggcgcc cagcgccagg gtcagagccg ataacttgag
    21961 tgcagatcga atacgcatgg cgcgggtact ccgtaacaac tgatagaaaa cggcgtagca
    22021 aagctacgcc gatgtgttgc aaatcttata gaccctattg tttaagcgtc aatttgtttg
    22081 acattggggt tagtacccgg tcgacacgcc gtgagacgct tgcggaacag ataggacagg
    22141 aatcggtcag gcaatatatt tgtccatggt gttgacggat ttgtccacgt gtatgtatcg
    22201 ttggaccaca aaaaagcggt cgcgcccccg ggcgcgaacc acaccgggcg acatggcccg
    22261 ataaccgaag gagacaatat gaaatcccgc atcgcagtgg ctgcgctcgc cttggcgctg
    22321 gccggggcgg caggccgttc cgccgcccag gctgccgaca ccatcgagct gaaggtcgct
    22381 gattcattcc ctgccggaca ctacctggtc gagctcatgc tcaagccctg gatggccgac
    22441 gtcaccaagc gcaccaacgg caaggtcaag ttcagctatt tccccaacca gcaactgggc
    22501 aaggccaccg acctgctcaa cctgacgcag tcgggcgtgg tcgacatcgg ctacatcgcg
    22561 ccctcgtatg cgtccgacaa gatgccgttg tcggccgtgg ccgaattgcc gggcgagttc
    22621 acccgcagct gcacgggcac catggcctac tggaagacgg cgcgcggcgg gatgctgagc
    22681 aaggccgaat accagcccaa cggcatccgc ctggtgatgg ccgtgctgct gccgccctac
    22741 aagatcttca ccgccaagca gaaggtcgag accgtgcagg acatccaggg cctgaagctg
    22801 cgcaccaccg gcggcgcgca ggacatcacc gcgcgcaccg tcaacgcggt gccggtgcgc
    22861 atgtcggcgc ccgacgccta tcaggcgctg tcgcgcggca cgctcgacgg cctgctcttc
    22921 cccgatgaga gcatctcgtc ctacggcctg gccaagctgg tcaagcacgc cac
//
LOCUS       DASWPO010000044         5930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2419711, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000044 DASWPO010000000
VERSION     DASWPO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419711"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGC69_02690"
     CDS             <1..240
                     /locus_tag="VGC69_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEY0294225.1"
                     /translation="EITLMGDGRKLAGHVESISLGIADRDRSTSANLLPNVNPTFNWV
                     RLAQRIPVRIKLDKVPDDVLLVAGRTATVAIVEKP"
     gene            237..1679
                     /locus_tag="VGC69_02695"
     CDS             237..1679
                     /locus_tag="VGC69_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0294226.1"
                     /translation="MNARLASTLAALLAALALAGCAPVGPDYRLPDQAVANRGTANAA
                     FRQQAADARLYSRDEAPAQWWRLYNDPQLDDLVRQALQANADLRQAAANLERAQAVAD
                     QARGKQDIETRVDANPFYGHQAGTQFLQPGLTLPNEWMYSASAGVSYQVDLFGQIHRA
                     IEAANADSQAAQAALDAARVTVAAETARAYASLCAAGMQLDSANRSVQVQRESLEAVR
                     TLAGNGRGTALDVTRAQAQLDQLQANLPPFQARQRSAFYLLAALTGRTPADMPQSLLA
                     CKTPPRLTHAIPVGDGALLLRRRPDVRQAERSLAAATARIGVATADLYPKITLGLSAM
                     SLGPADIVGQGSTINWSMGPLISWTLPTAGAHARVAQARAQAKGALARFDAVVLKSLQ
                     ETETALTVYARELDRVQALQAARQQSGQAARQARQLLAAGKTDYLSVLDAERTLAGAQ
                     SALAASQAELSADQIAVFLALGGGWEVNAP"
     gene            complement(1666..2370)
                     /gene="ypfH"
                     /locus_tag="VGC69_02700"
     CDS             complement(1666..2370)
                     /gene="ypfH"
                     /locus_tag="VGC69_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEY0294227.1"
                     /translation="MGSSSSIEFYPNPGLEARLLFVLLHGVGGCPDNLEKLGLALRVA
                     FPSSVVMIPEGFEVFDGGGTGRQWFSTRGVSEENRPERVAQALPALNDYVRAAQRRLK
                     LKAADTALGGFSQGAIMALEAVQADERLAGRVLAFSGRYAALPDAAPRYTTIHLLHGA
                     DDVVMPVTHAQAAQHRLAQLQGDATIDVASRVGHELHPALVQRAIVRLQTCVPLRSWE
                     AAMGHDQAPPPDATVH"
     gene            complement(2465..3715)
                     /locus_tag="VGC69_02705"
     CDS             complement(2465..3715)
                     /locus_tag="VGC69_02705"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY0294228.1"
                     /translation="MHITVIGAGVIGVTSAYFLARQGHDVTLIEARSGPAEVSSHANG
                     GQLSYSYVAPLAGPGVLPSVPGWLLRGDSPLRWRPRLDPRQWRWCLQFAMACNAARAE
                     ASTASLQALAYLSRDVLHALLEQEPLEFAHVRNGKLIAYRDAALLDKARAQVAYQARL
                     GAEQQVLDAAQTLQREPALASLGSKLAGAVYTPSEEAGDCRLFTEGLFDRLPRMGNVQ
                     CLMNAAVQGFDRRGDRRIAAVRTADGVVTTDAVVLAAGVATPALLRGLGGDAPIYPLK
                     GYSLSVPVAPDDPAAPMISVTDYQRRIVYARLGGVLRIAAMVDIDGEPGLVAERVALL
                     KRQVNEAFPALDLTYAEAWAGQRPATPTGVPLVGRSRVADNVWVNAGQGALGFTLACG
                     SATLLTAQVSSLSLPLDARAFLPR"
     gene            complement(3846..4286)
                     /locus_tag="VGC69_02710"
     CDS             complement(3846..4286)
                     /locus_tag="VGC69_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0294229.1"
                     /translation="MQLNHFTDFGLRAMMYLTQCQGREKRVTIPEIAERFRISRNHLM
                     KVVHFLSQSGWIVATRGKGGGLELARPPEHYKLGQMVHGLERQGSLTDCSDPSCELHG
                     LCLLADVLEDELRAFYASLDRHTLADLVREPTRTAIIRLHRAAA"
     gene            4493..5671
                     /locus_tag="VGC69_02715"
     CDS             4493..5671
                     /locus_tag="VGC69_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0294230.1"
                     /translation="MGFAPLKRFVVLDLSRVRSGPAAVRQFADWGADVIKIEEPSEVK
                     DDKPGGAAKFSDYQNTHRNKRSLTLNLKHPEGKAVFLDLVKRADVLVENYRPSVKAKL
                     GIDYASLRKINPRLVYASISGFGQDGPYADRPGLDQIAQGLGGLMSVTGEAGRGPMRA
                     GIPIADLSAGLFAAIGILIALLDREQTGEGRWVHTSLLQAQVWMMDFQATRWLIDGEV
                     PVQSGNDHPTSSPTGVYPTADGHINIAAMGSGMFTRLCEVLGMPELARDPRFVEAGSR
                     VENRMDLNREIISRTRTYSSADLIERLNAAGVPCGPILSMDGTFENEQVRHLNMVHHV
                     RHPSKGDMAIMGQPMIFDGTDLPQARPAPETGAHTDEILAWIGLDDAQRASLRATGAI
                     "
     gene            5711..>5930
                     /locus_tag="VGC69_02720"
     CDS             5711..>5930
                     /locus_tag="VGC69_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0294231.1"
                     /translation="MLRNKTPTEPIFGSLARPDNLPDEIAQQIRQKILSQAFAPGQRL
                     PTELELAQLFGVSRNVVREAIARLKLSGY"
BASE COUNT         1031 a         2158 c         1900 g          841 t
ORIGIN      
        1 gagatcacgc tgatgggcga cgggcgcaag ctcgccggcc atgtcgaaag catttcgctg
       61 ggcattgccg accgcgaccg cagcaccagc gccaacctgc tgcccaatgt gaacccgacc
      121 ttcaactggg tccgcctggc gcagcgcatc cccgtgcgca tcaagctcga caaggtgcca
      181 gacgacgtgc tgctggtggc cggccgcacc gccaccgtcg ccatcgtcga aaagccatga
      241 acgcccggct cgcctccacc ctggctgcgc tgctggccgc gctggccctg gccggctgcg
      301 cgcctgtcgg tcccgactac cggctgccgg accaagctgt cgccaaccgg ggcacggcca
      361 acgcggcctt tcgccagcag gccgccgacg ccaggctcta cagccgcgac gaagcgcccg
      421 cgcaatggtg gcgcctctat aacgatccgc agctcgacga cctggtgcgg caggccctgc
      481 aggccaacgc cgacctgcgc caggcggccg ccaacctcga acgcgcccag gccgtcgccg
      541 accaggcgcg cggcaagcag gacatcgaaa cccgcgtcga cgccaacccc ttctacggcc
      601 accaggccgg cacgcagttc ctgcagcccg gcctgaccct gcccaacgaa tggatgtaca
      661 gcgccagcgc gggcgtgtcc taccaggtcg acctgttcgg ccagatccac cgcgccatcg
      721 aggccgccaa tgccgactcg caggccgccc aggccgcgct cgacgccgcg cgcgtcaccg
      781 tggcggccga gacggcgcgc gcctatgcct cgctctgcgc cgccggcatg cagctggact
      841 cggcgaaccg ctcggtgcaa gtgcagcgcg agtcgctcga ggccgtccgg acgctcgccg
      901 gcaacggccg cggcacggcg ctggacgtca cgcgcgccca ggcgcagctc gaccagctgc
      961 aggccaacct gccgcccttc caggcgcgcc agcgcagcgc gttctacctc ctggccgcgc
     1021 tcaccggccg cacgcccgcc gacatgccgc aaagcctgct ggcctgcaag acgccgccgc
     1081 gcctgacgca tgccatcccg gtcggcgacg gcgccctgct gctgcgccgc cggcccgacg
     1141 tgcgccaggc cgaacgcagc ctggccgccg ccaccgcgcg catcggcgtg gccacggccg
     1201 acctctatcc caagatcacg ctgggcctgt cggccatgtc gctcggcccc gccgacatcg
     1261 tcggccaggg cagcaccatc aactggagca tgggcccgct gatttcgtgg accctgccca
     1321 cggccggcgc gcacgcccgc gtggcccagg cccgggcgca agccaagggc gccctggcgc
     1381 gcttcgatgc cgtggtgctg aagtcattgc aggaaacgga aaccgcgctg accgtctacg
     1441 cccgggagct ggaccgcgtc caggccctgc aggccgcgcg ccagcaaagc gggcaggccg
     1501 cccgccaggc gcgccagctg ctggccgcgg gcaagacgga ttacctgagc gtgctcgacg
     1561 ccgaacgcac gctggccggc gcgcaaagcg cgctggccgc ctcgcaggcc gaactgtcgg
     1621 ccgaccagat cgccgtgttc ctggccctgg gcggcggctg ggaagtcaat gcaccgtagc
     1681 gtccggaggc ggcgcctggt cgtggcccat ggccgcctcc cagctgcgca gcggcacgca
     1741 ggtctgcagg cggacgatgg cgcgctgcac cagcgccgga tgcagctcgt gccccacgcg
     1801 cgacgccacg tctatcgtgg catcgccctg caactgcgcc aggcggtgct gcgccgcctg
     1861 cgcatgggtc acgggcatca cgacatcgtc ggccccgtgc agcagatgta tcgtggtgta
     1921 gcgcggcgcc gcgtccggca aggccgcata gcggcctgaa aaggccagga cgcggccggc
     1981 cagccgctcg tcggcctgca cggcctccag cgccatgatg gccccctgcg aaaagccccc
     2041 cagcgccgtg tccgccgcct tgagcttgag gcgccgctgc gcggcgcgca cataatcgtt
     2101 gagcgcgggc aaggcctgcg ccacgcgctc cggccggttt tcctcgctga cgccgcgcgt
     2161 cgaaaaccac tggcgtccgg taccgccgcc gtcgaacacc tcgaaccctt ccggaatcat
     2221 caccaccgag ctgggaaacg cgacgcgcag cgccaggccc agtttctcga gattgtcggg
     2281 acaaccgccc acgccatgca gcaggacgaa cagcagccgg gcctcgaggc cggggttggg
     2341 ataaaactcg atggaagacg acgagcccat ggaaagcgcg ctccggaaaa acacgacgcg
     2401 ccgccgcggc gccccttcag gaaagggatt ctacgccgcg tcgcccgggc cgccagccga
     2461 ggtctcagcg cggcagaaag gcccgcgcgt ccagcggcag cgacaaggac gacacctggg
     2521 ccgtcagcaa ggtggcgctg ccgcaggcca gcgtgaaacc cagcgcgccc tgcccggcgt
     2581 tgacccacac attgtcggca acccggctgc gcccgaccag cggcacgccc gtgggcgtgg
     2641 ccgggcgctg tccggcccag gcctcggcat aggtcaggtc cagcgccgga aaagcctcgt
     2701 tcacctgccg cttgagcagc gccacgcgct cggccaccag gccgggctcg ccgtcgatgt
     2761 ccaccatggc ggcgatgcgc agcacgccgc ccagccgggc atagacgatg cggcgctggt
     2821 agtcggtcac gctgatcatc ggcgccgccg gatcgtccgg cgcgacaggc acgctcaggc
     2881 tgtagccctt caagggatag atgggcgcat cgccgcccag cccgcgcagc agcgccggcg
     2941 tggccacgcc ggccgccagc accacggcat cggtcgtgac cacgccatcg gccgtgcgca
     3001 cggccgcgat gcgccgatcg ccccggcgat cgaagccctg caccgcggcg ttcatcaggc
     3061 actgcacatt gcccatgcgc ggcagccggt cgaagagccc ctcggtgaac agccggcaat
     3121 cgcccgcctc ttcgctgggc gtgtacaccg cgccggccag cttggaaccc aggctggcca
     3181 gggccggctc ccgctgcagc gtctgcgcgg catcgagcac ctgctgctcg gcgcccagcc
     3241 gcgcctggta ggccacctgc gcgcgggcct tgtccaacag ggccgcgtcg cgataggcga
     3301 tgagcttgcc attgcgcaca tgggcgaatt ccagcggctc ctgctcgagc agggcatgca
     3361 gcacatcgcg gctcaggtag gccagggcct gcaggctggc cgtggaggcc tcggcccggg
     3421 cggcgttgca ggccatcgcg aactgcaggc accagcgcca ttggcgcggg tccagccggg
     3481 gccgccagcg caacggcgaa tcgccgcgca gcagccagcc cggcacgctg ggcagcacgc
     3541 ccgggccggc cagcggcgcc acgtagctgt agctcagctg cccgccattg gcatggctgg
     3601 acacctccgc gggcccggac cgggcctcga tcagggtcac gtcatgccct tgccgggcca
     3661 ggaagtaggc cgaagtcacg ccgatgacgc cggccccgat cacggtgatg tgcatagtgt
     3721 gttccgcaat gccgatatgg ccggtattgt ggaatcaggc gacgcctgtg cgccaatagc
     3781 gaacccgcca cccgccataa cctggactta tgggctgggg cggccgccgg gccgggccgg
     3841 ccgcgtcagg cggcggcgcg atgcagcctg atgatggcgg tgcgggtcgg ctcgcgcacc
     3901 aggtcggcca gcgtgtggcg gtcgagcgac gcatagaagg cgcgcagctc gtcctcgagc
     3961 acgtcggcca gcaggcacag gccgtgcagc tcgcaggacg gatcgctgca atcggtcagc
     4021 gagccctggc gctcgagtcc gtggaccatc tgtcccagct tgtagtgctc ggggggccgg
     4081 gccagctcca gcccgccgcc cttgccgcgc gtggccacga tccagccgct ctgcgacagg
     4141 aaatgcacca ctttcatcag gtggttgcgg gagatcctga agcgctcggc gatctcggga
     4201 atggtcacgc gcttttcgcg gccctggcat tgcgtcaggt acatcatggc gcgcaggccg
     4261 aaatcggtga aatggttcag ttgcatggtt gccgcgatat aggtatttca gatgacggat
     4321 tgaagcgtac atccgacacc agtactgttc ccgctccgtc ccgccgaaga cttgatcttg
     4381 ctcaaatcaa cgtcataaat acttcattaa agggttatcc ctcatgtccc cgcttaaata
     4441 tccaacttag gatgtccaac atcctacttt aagaataatg aggagacgcc ttatgggctt
     4501 tgccccgctg aaacgctttg tcgtcctgga cctgtcccgc gtccgatccg ggcctgccgc
     4561 ggtacgccaa ttcgctgatt ggggcgcgga cgtcatcaag atcgaagagc ccagcgaagt
     4621 caaggacgac aagccgggcg gcgcggcgaa gttcagcgac taccagaaca cccatcgcaa
     4681 caagcgcagc ctgaccctga acctgaaaca tcccgagggc aaggcggtat tccttgacct
     4741 ggtcaaacgc gcggacgtac tggtcgagaa ctaccgccct tccgtcaagg caaaactagg
     4801 catcgattac gcttcgctac gcaaaatcaa cccccgcctg gtctatgcca gcatttctgg
     4861 cttcggccag gatggcccgt atgccgaccg ccccgggctc gaccaaatcg cccagggcct
     4921 gggcggcctg atgtcggtca ccggcgaggc cggccgagga cccatgcggg cgggaatccc
     4981 gattgccgac ctcagcgccg gattattcgc cgccatcggc atcctgattg ccctgctgga
     5041 ccgggaacaa accggtgaag gccgatgggt gcacacctcg ctactgcaag cccaggtatg
     5101 gatgatggac ttccaggcaa cgcgctggct gatcgatggg gaggtccccg tccaatcagg
     5161 caacgaccac cccaccagct cgccgaccgg cgtctacccc acggcggacg gccacatcaa
     5221 tatcgcagcc atggggtcgg gcatgttcac ccgtctttgc gaagtgctgg ggatgccgga
     5281 actcgcgcgc gatccgcgct tcgtcgaggc cggatcgcgg gtggaaaacc gcatggacct
     5341 gaaccgggaa atcatctccc gcacacgcac ttactccagc gcagacttga tcgaacggtt
     5401 gaatgcggcg ggcgttccct gcggaccgat tctctccatg gacggcacat tcgagaacga
     5461 acaggttcgg catctgaaca tggtccacca tgtacgccat ccctcgaagg gagatatggc
     5521 gatcatggga caacccatga tcttcgacgg aaccgacctg ccgcaagcgc gaccagcccc
     5581 cgagacgggc gcgcataccg atgaaattct ggcttggata ggcctggacg acgcgcaacg
     5641 cgccagtttg cgcgccaccg gagccatatg acagtatcgg gctctctcaa ctccatcccc
     5701 cgccgctcct atgctccgca acaaaactcc gaccgagccg attttcggtt ccctcgcccg
     5761 gccagacaac ttgcccgacg agatcgcgca gcagatccgc caaaagatcc tgagccaggc
     5821 cttcgcgccg ggtcaacgcc tgcccaccga attggagctc gcgcaactgt tcggcgtcag
     5881 ccgaaacgtc gtacgcgagg ccattgcaag gctcaaatta tccggctaca
//
LOCUS       DASWPO010000045         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2241432, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000045 DASWPO010000000
VERSION     DASWPO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241432"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VGC69_02725"
     CDS             <1..411
                     /locus_tag="VGC69_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEY0294232.1"
                     /translation="DGEPGGTAGQPILRAIDGQGLDRVAVLVVRWFGGIKLGAGGLVR
                     AYGGCAANCLRLGERLELIDTAVAHCRCDFAELPILRARLAALGVAVREETFDERGAM
                     LALDVPRTQLIELEQAVANITRGRGGWRVEGEAD"
     gene            complement(412..1617)
                     /locus_tag="VGC69_02730"
     CDS             complement(412..1617)
                     /locus_tag="VGC69_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294233.1"
                     /translation="MPDKRIGVVVLLGITQTISWASSFYIPGVLAEPMARDLGVTPST
                     IYAALSFSLMISALLGPHAGRAIDRWGGRPVLCATNLVFAAGLALLGLAQGGLGLFAA
                     WAVLGIAMGSGLYEAAFATVVRYYGREARGAITGITLIGGFASTVGWPLSMLMESHLG
                     WRGACFAWAVLQLLLCLPLNSRLPAAPALRAVAATAVTAAATVSRMAQKPQWHGRMKS
                     FVLLAAMCTLTLFIGTAATAHLPRLLEASGLTLAAAASAGALMGPSQVGARVLELTVL
                     RRVHPMFPARLAAVLIPVSAALLLALGSPAAAMFTVVLGAGNGLFTIARGTLPLAIFG
                     AQGYGALQGMLMVPARVAQGLAPWLFGLLIEHWGVQALWLLAALSLIALGTLLALRIE
                     SPAAAPARA"
     gene            complement(1716..1825)
                     /locus_tag="VGC69_02735"
                     /pseudo
     CDS             complement(1716..1825)
                     /locus_tag="VGC69_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
BASE COUNT          303 a          610 c          711 g          201 t
ORIGIN      
        1 gacggcgagc cgggcggcac ggcgggccag cccatcctgc gcgccatcga cggccagggg
       61 ctggaccgcg tggccgtgct ggtggtgcgc tggttcggcg gcatcaagct gggggcgggc
      121 ggcctggtgc gcgcctacgg cggctgcgcg gccaactgcc tgcggctggg cgagcggctg
      181 gagttgatcg acaccgccgt ggcgcattgc cgctgcgatt tcgccgagct gcccatcctg
      241 cgcgcgcgcc tggccgccct gggcgtcgcg gtgcgcgagg aaaccttcga tgagcgcggc
      301 gcgatgctcg cgctggatgt gccgcgcacg cagctgatcg agctggagca ggccgtggcc
      361 aacatcaccc gcgggcgcgg cggctggcgg gtcgagggcg aagcggactg atcaggcccg
      421 ggccggcgcc gcggccggcg attcgatgcg cagcgcgagc agcgtgccca gggcgatcag
      481 gctcagcgcg gcgagcagcc acagggcctg cacgccccaa tgctcgatca acaggccgaa
      541 cagccagggg gcaagcccct gggccacccg agccgggacc atcagcatcc cctgcagggc
      601 gccgtagccc tgggccccga agatcgccag cggcaaggtg ccccgggcga tggtgaacag
      661 gccgttgccc gcgccgagca cgaccgtgaa catggcggcg gcgggcgagc ccagggccag
      721 cagcagggcg gcggacacgg gaatgagcac cgcggccagg cgcgcgggga acatgggatg
      781 cacgcggcgc agcacggtca gctccagcac ccgcgcgccc acctgggacg gccccatgag
      841 cgcgccggcc gatgcggcgg cggccagggt gaggccgctg gcttcaagca ggcggggcag
      901 gtgcgcggtg gccgccgtgc cgatgaaaag cgtcagggtg cacatggcgg ccagcaggac
      961 gaaagacttc atgcggccat gccactgcgg cttttgcgcc atgcgcgaga cagtggcggc
     1021 cgccgtcacc gcggttgccg ccactgcgcg cagcgccggc gcggcgggca gccggctgtt
     1081 gagcggcagg cacagcagca gctgcagcac ggcccaggca aagcaggcgc cgcgccagcc
     1141 caggtgggat tccatcagca tggaaagcgg ccagcccacg gtgctggcga agccgccgat
     1201 cagcgtgatg ccggtgatcg cgccgcgcgc ttcccggccg taatagcgca ccaccgtggc
     1261 aaaggccgct tcatagaggc cgctgcccat ggcgatgccc agcaccgccc aggcggcgaa
     1321 caggcccagg ccgccttgcg cgagacccag caaggccagg cccgccgcaa agaccaggtt
     1381 ggtcgcgcac agcaccggcc ggccgcccca gcggtcgatg gcgcggccgg catgggggcc
     1441 cagcagcgcc gagatcatca gggaaaagga cagcgccgcg taaatcgtcg acggggtcac
     1501 gcccaggtcg cgcgccatgg gctcggccag cacgccggga atgtagaacg acgaggccca
     1561 ggagatggtt tgagtgatgc ccaggagaac gaccacgccg attcgtttgt caggcatggt
     1621 gctgtccgga ggcgggattc acgatgggcc gcggtaatct gggaaagggc agcggggaag
     1681 gcgcagcgag gcgggcgtcg gcggccggcc taggcgggca ggatgtccag cacggtttcc
     1741 ttgggacggc acaggcgcgt gcccagcggc gtgacgacga aagggcgctc gagcaggatg
     1801 gggtgctgtt caatgaagtc cagca
//
LOCUS       DASWPO010000046         7549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2777403, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000046 DASWPO010000000
VERSION     DASWPO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2777403"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VGC69_02740"
     CDS             complement(<1..582)
                     /locus_tag="VGC69_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEY0294234.1"
                     /translation="MTSVFRAPLFGMLRTQGLALLLALAALLAWQPASAQLRVDISGT
                     GATQYPVAIADFAVDDAHGRALADVIRADLNRTGQFRLINAAGANLNVDSQVSYDDWR
                     GRGADFLAYGSILRSPDGRYDVRYRLGDDVKKVQLDGVAFSGTEQELRRVAHQIADRI
                     YEKITGVRGVFSTRIAYVLKQGNTYELQVADADG"
     gene            complement(596..1675)
                     /gene="tolA"
                     /locus_tag="VGC69_02745"
     CDS             complement(596..1675)
                     /gene="tolA"
                     /locus_tag="VGC69_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEY0294235.1"
                     /translation="MKRPSTPKRATSPDAATKDNRKALGLAVLVHVLLLLALIVGLDW
                     HSEAPPGPVQVQLWAKGDAPNSPPPTPQPQPQPQPEPQPAPPPPPPPPPPPPKPQPEV
                     RPPDQPDPEIALEQARKKREQEEKDRQAAEEAKKEAAEKAAAEQKAKEEAAQKAAADK
                     AAAEKAAADKAAAEQKAKDDAAKKAAADKAAADKAAADKAAAEKAAADKAAAEEKAKE
                     DAAKKAAADAAAKKAAADKALRDAFKNDILGNGGMPNGNADRNQTGGGGGRDNGYASK
                     VRACVQPGVAYPTPARAGSANPTAEYRVQLNSSGQVTGVALTSSSGNSVFDRAVESGI
                     RRCNPFPKPSIGPYPPLIDVVYQMY"
     gene            complement(1705..2166)
                     /locus_tag="VGC69_02750"
     CDS             complement(1705..2166)
                     /locus_tag="VGC69_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEY0294236.1"
                     /translation="MPSARSGGRHGRRMKSDINVVPYIDVMLVLLVIFMVTAPLITPG
                     QIELPSVGQASDVPVKPLEVQINADGKIALRLREPGATPVDIARPELVAQVRSRITAD
                     TPVVIAADGKVPYATVVKVMDELRSNGISRLGLLVDQGGSDKQPAAAPRKR"
     gene            complement(2166..2846)
                     /gene="tolQ"
                     /locus_tag="VGC69_02755"
     CDS             complement(2166..2846)
                     /gene="tolQ"
                     /locus_tag="VGC69_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY0294237.1"
                     /translation="MQASNDMSLLTLVSHASFPVQLIMLLLLGISIVSWTYIFGKRMT
                     IKRANKQTLRFEDDFWSGGDLAVLQQAVATRRDEQGPLARIFDAGMTEFLKSRRAAPA
                     GDINAALDGPRRAMRAAYQREMDALESHLNFLASAGSVSPYIGLLGTVWGIMHAFIGL
                     SNMQQATLASVAPGIAEALIATAIGLFAAIPAVVAYNRYTNDIDRLSIRFDSFIDEFL
                     NILQRQVR"
     gene            complement(2850..3275)
                     /gene="ybgC"
                     /locus_tag="VGC69_02760"
     CDS             complement(2850..3275)
                     /gene="ybgC"
                     /locus_tag="VGC69_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY0294238.1"
                     /translation="MTDLTSSKEFVFGIRVYYEDTDAGGVVFYANYLKFFERARTEWL
                     RTLGFHQSDMAGRLQRMFVVAGLDMDYRKPARLDDLLTIRSRVTRLGRASIHFAQRAE
                     RQGELIASGNIQICCVDTANLRPAELPADVRASLQPIQE"
     gene            complement(3341..5083)
                     /locus_tag="VGC69_02765"
     CDS             complement(3341..5083)
                     /locus_tag="VGC69_02765"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0294239.1"
                     /translation="MRASQYHLNTLKEAPAEAEVASHQLMTRAGMIRKLAGGVYTYMP
                     LGLRVIRKIENIVREEMNASGAIELLMPMVQPAELWQESGRYEQYGPELLRFKDRHDR
                     DFVLQPTSEEVITDIARNEIHSYRQLPLNFYHIQTKFRDERRPRFGLMRGREFTMKDA
                     YSFDRDEAGAQRSYDIMYAAYMRIFKRLGLEFRAVAADTGSIGGSRSHEFQVIADTGE
                     DLLVYNPDSDYAANIELAEAVSLLPGRAEPAEALAAVPTPGAAKCEEVAALLGLPLDR
                     TVKSIVLATDPKDDNPDGPAQIWLLLLRGDHELNEIKAGKIEGLAGFRFATEAEIVEH
                     FGCKPGYLGPIKTVRPVRVIADRTVADMADFVCGANQVDMHYRGANWGRDLPEPEQVT
                     DLRNVVEGDPAPDGRGRLAIQRGIEVGHVFYLGTKYSEALKATFLDETGKPALLEMGC
                     YGIGVTRIAGAAIEQNHDGRGIIWPRAIAPFEVVICTVGWGKSETVRNTATELYEALR
                     ARGVEVILDDRDARPGVMFAEWELIGVPLRVTVGDRGLKDGLIELQARNETEAGKVPV
                     GNALEQTLAKLETL"
     gene            5157..5738
                     /locus_tag="VGC69_02770"
     CDS             5157..5738
                     /locus_tag="VGC69_02770"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY0294240.1"
                     /translation="MLDREGYRPNVGIILVNGKNEVFWGKRIREHAWQFPQGGIKHGE
                     SPVQAMYRELHEEVGLKPEHVRILGRTRDWLRYEVPDNFVRREWRGHYKGQKQIWFLL
                     RLVGHDCDVCLRASSHPEFDAWRWSQYWVPLDAVIEFKRDVYTRALNELSAILFRRHH
                     ETRYLRQRVHGARPAESIDPPSAPPDGHAHIVG"
     gene            5719..6405
                     /locus_tag="VGC69_02775"
     CDS             5719..6405
                     /locus_tag="VGC69_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0294241.1"
                     /translation="MRILLVEDEQEMAAWLLRALTQGGFLPDHAPDARTAESLMAGNE
                     YDVVVLDLRLPDKHGMALLREMRDRDDRTPVLLLTAQGGLQDRVRGLNLGADDFLTKP
                     FALEELEARITALVRRSRGRQHPRLQCGSLSYDSESRAFTLDGSLLFLTPREHAALAA
                     LLTRSGYPVDKVQLFGKVFTHDSEANLDAIEVVLHRLRKKLAGSDVHIVTVRGLGYML
                     ESLARTTAET"
     gene            6405..>7549
                     /locus_tag="VGC69_02780"
     CDS             6405..>7549
                     /locus_tag="VGC69_02780"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0294242.1"
                     /translation="MALAGWWRGKPRFGIRSLLIMLLLPVLVVLLVLDSLNDYRTLSS
                     TANTAYDTALAESARLLSSGLLLGDDGQIEVAPPLYAQFLVRAESRLHRYFRIEDADL
                     PDIIVGLRSVRGRTLAGMVDLPGPPLWPADPGGMVFFDTFYRGEPMRAVAVLRTVHRG
                     QTSRQALVVVAESATWRSGVELHARGQEFWRDIGTLLLESLLLWLGVHWALRPLRRLS
                     REVDARSPEDLTPLDERGVPSEVVPLVDAVNLHLKRHRQMLDERAQFLDDASHQLRTP
                     LAIISTQAQYALRERDPAQAREALQAIVEQLGRSRRLTEQLLSLAHASQAEAMQHEVV
                     DLARIARDAVMQYLPLAHEKGQDLGWVDARPGEAAPAPVFASEIELH"
BASE COUNT         1137 a         2397 c         2648 g         1367 t
ORIGIN      
        1 cccgtcggcg tcggccacct gcagctcgta ggtattgccc tgcttgagca cataggcgat
       61 gcgggtggaa aacacgccgc gcacgccggt gatcttttcg tagatgcggt cggcgatctg
      121 gtgggccacg cggcgcagct cctgctcggt gccggagaag gccacgccgt cgagctgcac
      181 tttcttgacg tcgtcgccca ggcggtagcg gacgtcgtag cggccgtcgg gcgagcgcag
      241 gatgctgccg taggccagga agtcggcgcc acggccgcgc cagtcgtcgt acgagacctg
      301 gctgtcgacg ttcaggttgg cgccggcggc gttgatcagg cggaactggc cggtgcggtt
      361 caagtcggcg cggatcacgt cggccagggc gcggccgtgc gcgtcgtcga cggcgaagtc
      421 ggcgatggcc accgggtact gggtggcgcc ggtgcccgag atgtcgacgc gcagctgggc
      481 gcttgcgggc tgccaggcca gcagggctgc cagcgccagc aagagcgcca ggccctgcgt
      541 gcgcagcatc ccgaacaagg gggcgcggaa gacggatgtc atgcggctat ctccttcaat
      601 acatttggta cacgacgtcg atcagcggcg gatacggacc tatcgacggc ttggggaagg
      661 ggttgcagcg gcggatgccg ctctccactg cccgatcgaa gacggaattg cccgaggaac
      721 tcgtcagggc aacacctgtg acctggccac tggagttcaa ctgtacccgg tattcggcgg
      781 tcggattggc cgacccggcg cgcgccgggg tggggtaggc caccccgggc tgcacgcagg
      841 cgcggacctt ggacgcgtag ccattgtcgc gcccgcctcc gccgcctgtc tggttgcggt
      901 cggcattgcc attgggcatg ccgccgttgc ccaggatgtc gttcttgaat gcgtcgcgca
      961 gcgccttgtc ggcggccgct ttcttggccg cggcatcggc agcggccttc ttggcggcgt
     1021 cctccttggc cttttcttcg gccgcggctt tgtcggcggc cgctttctcg gcggcggcct
     1081 tgtctgcagc cgccttgtcc gcggcggcct tgtcggcagc ggccttcttg gcggcatcgt
     1141 ccttggcttt ctgctcggcg gcggctttgt cggccgccgc tttttcagcc gcagccttgt
     1201 cggcggccgc cttctgggcc gcttcttcct tggctttctg ttcggcagcg gccttttcag
     1261 cggcttcctt tttggcctct tcggccgcct ggcggtcctt ttcttcctgt tcgcgcttct
     1321 tcctggcctg ctccagcgcg atctcggggt cgggctgatc gggcgggcgc acttcgggct
     1381 gcggcttggg cggcggcgga ggtggggggg gcggaggcgg cggagccggc tgcggttcag
     1441 gctgcggctg aggttgtggc tgcggcgtag gcggggggct gttgggcgcg tcgcccttgg
     1501 cccacaactg cacctgcacg gggcccggcg gggcctcgga atgccagtcc agtcccacga
     1561 tcagggccag cagcaatagc acgtgcacca ggacggccag gcccagggcc ttgcggttgt
     1621 ccttggtggc tgcgtccggc gacgtggcgc gcttgggggt agacgggcgt ttcattcgat
     1681 ggcgtgcggc ggcagtcccg ggggtcagcg tttgcgcggc gcagcggcgg gctgcttgtc
     1741 gctgccgccc tggtcgacca gcaggcccag gcggctgatg ccgttgctgc gcagttcgtc
     1801 catgaccttc acgaccgttg catagggcac cttgccgtcg gcggcaatga ccacgggggt
     1861 gtcggccgtg atgcgcgaac gtacctgggc caccagttcg gggcgggcga tgtcgaccgg
     1921 cgtggcgccg ggttcgcgca ggcgcagggc gatcttgccg tcggcattga tctgcacttc
     1981 cagcggcttg accggcacgt cggaagcctg gcccaccgag ggcagttcga tctgtccggg
     2041 ggtgatcagc ggcgcggtga ccatgaagat caccagcagc accagcatca cgtcgatata
     2101 gggaacgacg ttgatgtcgg atttcatgcg ccggccgtgc cggcctccag agcgtgcgga
     2161 aggcatcagc gcacctgccg ttgcagaata ttcaaaaatt cgtcgatgaa gctgtcgaag
     2221 cggatcgaca gccggtcgat gtcgttggtg tagcggttgt aggccaccac ggcggggatg
     2281 gcggcgaaca ggccgatggc ggtggcgatc agggcctcgg caatgccggg ggccaccgag
     2341 gccagcgtgg cctgctgcat gttggacagg ccgatgaagg cgtgcatgat gccccatacc
     2401 gtgcccagca ggccgatgta cgggctgacc gagccggccg aggccaggaa attcaagtgc
     2461 gattccagcg catccatctc gcgctggtag gcggcgcgca tggcgcggcg cgggccgtcg
     2521 agcgcggcgt tgatgtcgcc cgcgggcgcc gcgcggcgcg atttcaggaa ttcggtcatg
     2581 ccggcatcga agatgcgggc caaggggcct tgctcgtcgc ggcgggtggc gacggcctgc
     2641 tgcagcacgg ccaggtcgcc gcccgaccag aagtcgtctt cgaagcgcag cgtctgcttg
     2701 ttcgcgcgct tgatggtcat gcgcttgccg aagatatagg tccaggagac gatggagatg
     2761 cccaggagca gcagcatgat cagctgcacg gggaagctgg catgggaaac cagcgtgagc
     2821 aacgacatgt cgttggaagc ttgcatggat tattcctgga tcggttgcag tgaggcgcgc
     2881 acgtcggcgg gaagctccgc ggggcgcagg ttggcggtgt cgacgcagca gatctggata
     2941 ttgcccgagg cgatgagctc gccctggcgc tccgcgcgct gcgcgaagtg tatcgaagcc
     3001 cggcccagcc gtgtcacccg gctgcggatg gtcagcaggt cgtcgaggcg ggcgggcttg
     3061 cggtagtcca tatccagtcc ggcgacgacg aacatgcgct gcaggcggcc agccatgtcg
     3121 gattggtgaa aacccagtgt ccgcagccat tcggtacggg cgcgttcgaa gaacttcaag
     3181 tagttggcgt agaagacgac gccgccggca tcggtgtctt cgtaatagac acggataccg
     3241 aatacaaact ctttggagga cgtgagatcg gtcacgatgg gtaaaaaact agaccggacg
     3301 gctcggtggc agtccggccc gaatattacc gcgtatcgcg ttacagcgtt tcgagcttgg
     3361 cgagagtctg ttccagggcg tttccgaccg gcaccttgcc ggcctcggtc tcgttgcggg
     3421 cctgcaattc tattaagccg tctttcaggc cgcgatcgcc cacggtaacg cgcaggggta
     3481 ccccgatcag ctcccattcc gcgaacatga cgcccgggcg ggcatcgcgg tcgtccagga
     3541 tgacttccac gccgcgcgcg cgcagggcct cgtagagctc ggtggcggtg ttgcgcactg
     3601 tttcactttt gccccagccc accgtgcaga tcaccacctc gaagggggcg atggcgcggg
     3661 gccagatgat gccgcggccg tcgtggttct gttcgatggc ggcgccggcg atccgggtga
     3721 cgccgatgcc gtagcagccc atttccagga gggcgggctt gccggtctcg tccaggaagg
     3781 tggccttgag cgcctcggaa tacttggtgc ccaggtagaa cacgtggccc acctcgatgc
     3841 cgcgctggat ggccagcctg ccgcggccgt cgggggcggg gtcgccctcg accacgttgc
     3901 gcaggtcggt cacctgctcg ggttcgggca ggtcgcggcc ccagttggcg ccccggtaat
     3961 gcatgtccac ctggttggcg ccgcagacga agtcggccat atcggccacg gtgcggtcgg
     4021 cgatcacgcg caccgggcgc acggtcttga tggggcccag gtagccgggc ttgcagccga
     4081 aatgctcgac gatctcggcc tcggtggcga agcgaaagcc cgccaggccc tcgatcttgc
     4141 cggccttgat ctcattgagc tcgtggtcgc cgcgcagcag cagcagccag atttgcgccg
     4201 ggccgtccgg gttgtcgtct ttcgggtcgg tcgccagcac gatggacttg accgtgcggt
     4261 ccagcggcag tcccagcagg gcggccacct cctcgcactt ggcggcgccg ggggtgggca
     4321 cggcggccag ggcctcggcc ggctcggcgc gcccgggcag caggctgacc gcctcggcca
     4381 gttcgatatt ggcggcatag tcggagtcgg ggttgtagac cagcaggtcc tcgccggtgt
     4441 cggcgatcac ctggaactcg tggctgcggc tgccgccgat cgagccggtg tcggccgcca
     4501 cggcgcggaa ttccagcccc aggcgcttga agatgcgcat ataggccgcg tacatgatgt
     4561 cgtagctgcg ctgcgcgccg gcttcgtcgc ggtcgaagga ataggcgtcc ttcatggtga
     4621 attcgcggcc gcgcatcagg ccgaagcggg ggcggcgctc gtcgcggaac ttggtctgga
     4681 tgtgatagaa attcagcggc agctggcggt agctgtggat ttcgttgcgg gcgatgtcgg
     4741 tgatgacctc ttccgaggtg ggctgcagca cgaagtcgcg gtcatggcga tccttgaagc
     4801 gcagcagctc ggggccgtac tgctcgtagc ggcccgactc ctgccagagc tcggccggct
     4861 ggaccatcgg catcagcagt tcgatggcgc cggaggcgtt catttcctcg cgcacgatgt
     4921 tctcgatctt gcgaatgacg cgcaggccca gaggcatata ggtgtaaacc ccccctgcca
     4981 gcttgcggat catcccggcc cgggtcatca gttgatggct ggccacttct gcttcggcag
     5041 gagcttcttt aagggtattc aggtggtatt gggaggcgcg catagggagg tagacgaggc
     5101 tgatatacgt ataaaatcga ctgcaattgt attgaattca agtttagggg tggcccatgc
     5161 tcgaccgcga aggctaccgc cccaatgtcg gaatcattct cgtcaatggc aaaaacgagg
     5221 ttttctgggg caagcgtatc cgggaacatg cctggcagtt cccgcaaggt ggcatcaagc
     5281 acggcgaaag tccagtgcag gccatgtatc gcgaactcca tgaggaagta ggcctcaagc
     5341 ctgagcatgt ccgcatcctg gggcgcacgc gcgactggct gcgctatgaa gtgccggaca
     5401 atttcgtccg gcgcgaatgg cgtggccatt acaaaggcca gaagcagatt tggttcctgc
     5461 tgcgtctggt cggacacgat tgcgacgtct gcctgcgcgc gtcgtcgcat cccgagttcg
     5521 acgcctggcg ctggagccag tattgggtgc cgctcgatgc cgtcatcgag ttcaagcgcg
     5581 atgtctacac cagggcgctg aacgaactgt cggccattct tttccgtcgg caccacgaaa
     5641 cccgttatct gcggcagcgc gtgcatggcg cacgtccggc cgaatccatc gacccccctt
     5701 cggcacctcc ggacggccat gcgcatattg ttggttgaag acgagcaaga gatggccgcg
     5761 tggctgctcc gcgcgctcac gcagggcggc ttcctgcccg accacgcgcc cgatgcgcgc
     5821 acggccgaaa gcctgatggc gggcaacgag tacgacgtcg tcgtgctcga cctgcggctg
     5881 cccgacaagc acggcatggc gctgctgcgc gaaatgcgcg accgcgacga ccgcacgccc
     5941 gtgctgctgc tcacggccca gggcggcctg caggatcggg tgcgcggcct gaatctcggc
     6001 gcggacgact tcctcaccaa gcccttcgcc ctcgaagagc tcgaggcccg catcacggcc
     6061 ctggtgcgcc gcagccgcgg ccgccagcat ccccgcctgc agtgcggctc gctgtcctac
     6121 gacagcgaaa gccgcgcctt cacgctcgac ggctccctgc tctttctcac cccgcgcgag
     6181 catgcggccc tggcggcctt gctcacgcgc agcggctacc ccgtcgacaa ggtccagctc
     6241 ttcggcaagg tgttcacgca cgacagcgaa gccaatctcg acgccatcga ggtggtcctg
     6301 catcgcctgc gcaaaaagct ggccggcagt gacgtgcaca tcgtcacggt gcgcggcctg
     6361 ggttatatgc tcgaaagcct ggcgcgcacg actgccgaga cgtgatggcc ctggccggct
     6421 ggtggcgcgg caagccgcgc ttcggcatcc gttcgctgct gatcatgctg ctgctgcccg
     6481 tgctggtggt gctgctggtc ctcgacagcc tgaacgacta ccgcacgctc tcgagcaccg
     6541 ccaacacggc ctatgacacg gcgctggccg agtcggcccg tttgctcagc agcggcttgc
     6601 tgctgggcga cgacgggcag atcgaggtcg cgccgccgct ctacgcccag ttcctggtcc
     6661 gcgcggaaag ccggttgcac cgctatttcc gcatcgagga tgccgacctg ccggatatca
     6721 tcgtgggttt gcgatccgtg cgggggcgca cgctggccgg catggtggac ctgcctggcc
     6781 cgcccctctg gccggccgac cctggcggga tggttttctt cgataccttc tatcgcggcg
     6841 agccgatgcg cgccgtggcc gtgctgcgca ccgtgcaccg cggccagacg agccgccagg
     6901 ccctcgtggt ggtggcggag agcgcaacct ggcgctcagg cgtcgaattg catgcgcgcg
     6961 ggcaggagtt ctggcgcgac atcggcacgc tgctattgga aagcctgctg ctctggctgg
     7021 gcgtgcattg ggccttgcgc cccttgcggc ggctgagcag ggaagtggac gcccgcagcc
     7081 ccgaggacct gacgccgctg gatgagcgcg gcgtgccgag cgaggtcgtc ccgctggtcg
     7141 atgcggtcaa cctgcatctg aagcggcacc gccagatgct cgacgagcgg gcgcaattcc
     7201 tggacgatgc ctcgcatcag ctgcgcaccc cattggccat catttccacc caggcgcagt
     7261 acgccctgcg cgaacgggac ccggcccagg cgcgcgaggc cttgcaggcc atcgtcgagc
     7321 aactgggccg cagccggcgg ctgaccgagc agctgttgtc cctggcccat gccagccagg
     7381 ccgaggccat gcagcacgaa gtggtcgacc tggcccgcat cgcgcgcgat gcggtcatgc
     7441 agtatctacc cctggcccac gaaaaggggc aggatctggg atgggtcgac gcccgccccg
     7501 gcgaggcggc tcccgcgccg gtttttgcca gtgaaatcga gctgcatga
//
LOCUS       DASWPO010000047        15047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1438233, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000047 DASWPO010000000
VERSION     DASWPO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438233"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1629
                     /locus_tag="VGC69_02785"
     CDS             31..1629
                     /locus_tag="VGC69_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0294243.1"
                     /translation="MRVNLPVNDNEVRMREDQFLISRTDTKGRIIYANPAFVEMSGYS
                     RAELEGKAHNIVRHPDMPPEAFADLWRTLEKGNTWSGLVKNRRKDGSFYWVYATVTPI
                     YENGEVAAYSSVRIRPTDEQIDAADAFYAKLNAGKARGWQVRNGRPARTGVLGALSGL
                     SFPLRRGMRAHMFRTATLATVAAGAMGWYGLSARWAAMSGGEAWGAGAVAVLVAAAVA
                     ITQWRLNRRIARSMTSAGDLVLQVAAGNLTTKIGSNANDQAGGLALALEIMRKSLTNI
                     AQDVHAGIAQAVQAASGIATGNQLLATRTSDGSAALQGTAASMEEITSTVKQNADNAR
                     QASILADRSMDVARRGGSAVAQVVDTMHGISSSSSKIGDIVTLIEGIAFQTNILALNA
                     AVEAARAGEAGKGFAVVAGEVRSLAQKSAQAAREIKGLIEASVDRVNEGSMQVEQAGD
                     TMQEIVDAVQRVSDIISEISVASQQQASGIEQVDMAVQQMDGMTQENLSLVQQLGEAV
                     GQLNDQSADLDQSIRVFRLQPGQH"
     gene            complement(1642..2430)
                     /gene="fliR"
                     /locus_tag="VGC69_02790"
     CDS             complement(1642..2430)
                     /gene="fliR"
                     /locus_tag="VGC69_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY0294244.1"
                     /translation="MITFTMDQLNGWIGQFLWPFVRVLGLIIASPIFSESGIPTLVKI
                     GLSFMLTLAIAPAAGPMPAIDLNSYEAIMVVMQQTVIGLAMGLVMRVVFAAVQTGGEF
                     ISLQMGLSFASFFDPMQGATTSVLPQFLNIIATMLFLSFDGHLLLLAALAHSFDTLPV
                     GFDALNRNGWGILAEWGASIFSSGLLLALPLICALLITNLAMGVLNRAAPQLTVFTVG
                     FPISLISGLLLLASVLPHSGAYFEALFQQGYDTLGRLLTGLAGR"
     gene            complement(2467..2736)
                     /gene="fliQ"
                     /locus_tag="VGC69_02795"
     CDS             complement(2467..2736)
                     /gene="fliQ"
                     /locus_tag="VGC69_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY0294245.1"
                     /translation="MTAETVMTLAYQAMKISLAMATPLLLISLVVGLVISVFQAATQI
                     NEMTLSFIPKLLAICAGLVLLGPWLINLMVDYMRQIITQIPSLVS"
     gene            complement(2756..3481)
                     /gene="fliP"
                     /locus_tag="VGC69_02800"
     CDS             complement(2756..3481)
                     /gene="fliP"
                     /locus_tag="VGC69_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939091.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY0294246.1"
                     /translation="MALIGLALFPGSSLAQATLPALTATPGPGGSQTYSLSMQTMLLM
                     TSLTFLPAALLMMTGFTRIIIVLGLLRTAMGTQMSPPNNVLIGLALFLTFYTMSPVFD
                     KIYTDAYQPLSNNAISFETAVDRASQPLRTFMLHQTRENDLSLFADMAKQPAMSDPTQ
                     VPMRILVPAFITSELKTAFQIGFTIFIPFLIIDMVVASVLMALGMMMVPPATMSLPFK
                     LMLFVLADGWHLLLGSLAQSFYQ"
     gene            complement(3598..3936)
                     /gene="fliO"
                     /locus_tag="VGC69_02805"
     CDS             complement(3598..3936)
                     /gene="fliO"
                     /locus_tag="VGC69_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY0294247.1"
                     /translation="MMQDAVLRVTIVLVLVVAVILAMAWLARRAGFIPRQDASSLRMV
                     SRLSLGPRQHVAVIQVQDTWLVLGLTPTSITALHTLPAQAAPAAPEPATGEAFAQTLA
                     AKLGKVLKRP"
     gene            complement(3941..4459)
                     /gene="fliN"
                     /locus_tag="VGC69_02810"
     CDS             complement(3941..4459)
                     /gene="fliN"
                     /locus_tag="VGC69_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY0294248.1"
                     /translation="MADQNDRPAQPGATPPAASATDDWAEALAEQARTVPPTQADGLR
                     TAAEAAAEPAPAAAATAASTAQPATSVFKPLISNEGAASTTDIDLIMDVPVQLTVELG
                     RTRLTIKNLLQLGQGSIVELDALAGEPMDIFVNGYLIAQGEVVVVEDKYGIRLTDIIT
                     PSERLNRLNNRR"
     gene            complement(4452..5465)
                     /gene="fliM"
                     /locus_tag="VGC69_02815"
     CDS             complement(4452..5465)
                     /gene="fliM"
                     /locus_tag="VGC69_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY0294249.1"
                     /translation="MAYEAFLSQDEVDALLAGVTGEPQDSSPVPNNDPPGTRAYDLSS
                     PDRVVRRRMQTLELINERFARHMRTVLLNFMRRSADITVSSIRIEKYADFERNIPVPS
                     NLNIVQMRPLRGTAMLAYDANLVFLVVDSLFGGDGRYHMRVEGRDFTNTEQRIIRRLL
                     NLTLDSYGRAWDSVYPIEFEYVRSETHTKFANITGGNEVVVISSFHIEFGAQGGDLNI
                     CLPYSMIEPIRDILARPMQETALEEIDQRWTHQLSRQVRSADIDLVAEFVRIPSSVRE
                     LLAMKVGDVLPVEIPESITAHVNGVPLMECGYGAFNSQYALRVQNMIHHDDSDFNEAF
                     KHG"
     gene            complement(5468..6040)
                     /locus_tag="VGC69_02820"
     CDS             complement(5468..6040)
                     /locus_tag="VGC69_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY0294250.1"
                     /translation="MATTIKSPTKLGSVSSSSGLGFGGLLRPLIWLLVLIIVAALCIA
                     GTWFYLHWQSSRETNPVQLSVGQSSTPTQVSYTAPPAVTQIPAPIFMPLDPFTVMLED
                     KDSERLLHVALTLRLGDEQSRARIEKYLPEVRSRILMVLSDLSPQDIRTPEGKAGITK
                     SVAAAVSKPFAPIPDGQNVSDVLFTEFVVQ"
     gene            complement(6184..7677)
                     /locus_tag="VGC69_02825"
     CDS             complement(6184..7677)
                     /locus_tag="VGC69_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY0294251.1"
                     /translation="MTTALNLAALNAAAPQPAQAAAVQAAPACGANGNNPSFSQVLAG
                     QAGNGANQAPGSKAPPAQSADHSSNADNAGNGRPAAQAGDGDTGSATDKTAGNTSAKH
                     GNGKDDPADPADAASTALTNTAAVQALPQQPAADPQQAMQSAAPSLPQQALDIAAQVA
                     AVQSQVANRQSAAPAPAATEKHGTLPVLATASTASATSAAKPAASTALQTPALHASTP
                     TSQTRDSATLDPLADAAQQPAQPAATIAQTPAQMARQAAATDTSLAAATPAGQTAHAA
                     HAGAQAGDAGTPTADALQQLQAQQSAQQPAMPLPHAAGNTDAQASLAAMTAVPAAAAT
                     QTTAAGYALTPAPISINLVSSPVGSAQWNLDLGRQMVLLSHGTQRGGQTAELRLDPPD
                     LGPLRVTINLSDGVASAAFVSAHAAVRQALESAMPQLQHAFEQAGLSLGQTSVGEQGA
                     QSGQEQAARGRSGGGFAASEPAGGGSVSAVLQVAAPRDANALVDTFA"
     gene            complement(7705..8154)
                     /gene="fliJ"
                     /locus_tag="VGC69_02830"
     CDS             complement(7705..8154)
                     /gene="fliJ"
                     /locus_tag="VGC69_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEY0294252.1"
                     /translation="MPSQLPLETLIGLAREHADEAARSLGRLNRERQNAEHQLGMLQD
                     YRHDYLVRLQQAMQTGMSTSDCHNYQRFISTLDDAINQQVAALDTLNVNHEKSRLHWQ
                     QQQRKLNAFDTLAQREARAAQVVAERREQRASDEHSARLASQRHSFY"
     gene            complement(8147..9631)
                     /gene="fliI"
                     /locus_tag="VGC69_02835"
     CDS             complement(8147..9631)
                     /gene="fliI"
                     /locus_tag="VGC69_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEY0294253.1"
                     /translation="MNAPVQTEARPGADAPSVPLAERWKTQLEIGSIRAASTEPWLVT
                     GRIIRATGLVLHATGLRLPVGAACRIEIARGHDHWADAEVVGFDGHTLYLMPQADISG
                     LPPGARVVPAEPAVQRRVPLPKNAELNGNAKPQLGRHLPVGNALLGRVVDGAGRPLDS
                     LGPLVGAELAPLTALPINPLSRAPIDSVLDVGVRAINGLLTVGRGQRMGLFAGSGVGK
                     SVLLGMMARYTRADVIVVGLIGERGREVKEFIEHNLGPEGLARSVVVAAPADVSPLLR
                     LQGASYATRLAEHFRDQGLDVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAR
                     LPALVERAGMGSPGANGKAGSITAFYTVLAEGDDQQDPIADSARAILDGHVVLSRYLA
                     EAGHYPAIDIEASISRAMTSLITPQQFNLVRRFKQSISRYQRNRDLIAVGAYAAGNDP
                     QLDDAIARYPRLEAFLQQDVGENVGYEPTIAQLRAIIEPRDIHA"
     gene            complement(9628..10392)
                     /gene="fliH"
                     /locus_tag="VGC69_02840"
     CDS             complement(9628..10392)
                     /gene="fliH"
                     /locus_tag="VGC69_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEY0294254.1"
                     /translation="MSDRLPVDPHQPPLSHRAAWRRWEMMSFDEPEAAAEPEAADPAP
                     QPAGPSLEEIVQAAREEAYREAHQQGQADGHKQGYMQGLNEGRKAGHAEGYAAGHAEG
                     LAHGREQGKQEAVRLNTLVSRTAAAMGGLEEQMGQSLITLALDIAAQVLRGELAEHPE
                     SMLHAVREVLHLNPAVPQGVMRLWVHPDDLELVRLQLAEELKEANWRVLADESIERGG
                     CRAETPYGDIDATLHTRWRRVAASLGRNAAWEDSQP"
     gene            complement(10385..11398)
                     /gene="fliG"
                     /locus_tag="VGC69_02845"
     CDS             complement(10385..11398)
                     /gene="fliG"
                     /locus_tag="VGC69_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY0294255.1"
                     /translation="MTTPDGKTIDGLTRSAVMLMTLGEDAAAQVFRYLSAREVQMVGG
                     AMASLKQVTRRDVSNVLEEFRQEADQFLAVSVGSDDYIRSVLTKALGSDRAAGLIEDI
                     LEAGESGNGIDALNWLDATTVTELIGNEHPQIIATILVHLERDKAAGVLAMLSDRLRN
                     DVMLRIATFGGVQPTALAELTEVLNSMLAGQSAKRSKMGGVRTAAEILNMMNSSQEEA
                     VVSSLRERDADLAQKIVDEMFTFENLLEVEDRGIQLVLKEIDNEMLMVALKGAPEELR
                     NKFLRNMSSRAAEMLREDLDAQGPVRMSKVESEQKKIIQIARRLAEAGQITLGAQGDD
                     AYV"
     gene            complement(11385..13070)
                     /gene="fliF"
                     /locus_tag="VGC69_02850"
     CDS             complement(11385..13070)
                     /gene="fliF"
                     /locus_tag="VGC69_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY0294256.1"
                     /translation="MNQQAALGTTILARFPVLARIKALPKPILLGAAAALVALIAVAV
                     LWSRDPDYKVLFSNLDDRDGGAIVTALGQMNVPYKFNDNGTALLVPADRVYNTRMQLA
                     SQGLPRGGSIGFEIMDNARFGASQFTEQINYQRGLEGELARSIEAMNTVQHARVHLAL
                     PRQSLFVRERQAPTASVLISVYPGRSLSQAQVSAISYLVASSVPELNADNVSIVDQNG
                     RLLSAPQGEGRGMDADQLRFVREVEQRTVERILTILNPLVGPGNVHAQASADVDFSRH
                     EETSETYRPNQDPGQASIRSQQTSDSKQTGANAAQGVPGALSNQAPPNAQAPIVNPPA
                     QPGQPGAVNPQAAQAAQQTAAQQAAAQQQNVPTNDRHDATTNYELDRTISHTKAPVGV
                     LKRLSVAVVINNLPGKDGRDPQPLPPEELAKLTDLVKEAMGYSDNRGDSLNLVNSAFN
                     DAEPTVPFWRDPELIALAKTIIGWLIFAAVLLWLYRKIKPAAMDYFFPQVDPEVAKAE
                     RLEAERAAMTVAREREINRYEDNLQRARDMAMKDPRAVAMVLRSWMNNQDDNS"
     gene            13383..13730
                     /gene="fliE"
                     /locus_tag="VGC69_02855"
     CDS             13383..13730
                     /gene="fliE"
                     /locus_tag="VGC69_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY0294257.1"
                     /translation="MAVSGVSGVESMLQQMRALVEAAQKGVSDPSELSPSVPSQQAVS
                     FADELHQSIKRISDAQNASTAQAKAFELGDPRVSLNDVMIDMQKASIGFQTAMQVRNR
                     LVEAYREVSSISM"
     gene            complement(13737..14138)
                     /locus_tag="VGC69_02860"
     CDS             complement(13737..14138)
                     /locus_tag="VGC69_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294258.1"
                     /translation="MPAAPSEFPLPDAWLRQAMDIRHALAALAHPECRIRLRNTGDEL
                     DLGVRILGIDARSSLFFWRLGEAHADLLENEAELARLAALTLGFHGRIHDHKTGMQFH
                     AGPSTVLQFSDRSLLMITPFPQSLRQFSLDG"
     gene            complement(14155..14484)
                     /locus_tag="VGC69_02865"
     CDS             complement(14155..14484)
                     /locus_tag="VGC69_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY0294259.1"
                     /translation="MSGIGYPDPRRAAVAVSYDEHDASPRVVAKGYGKLADTIVRAAR
                     EHGLYVHESSELVGLLMQVDLDSHIPPQLYLAVAELLAWLYRLESRGLPGQAQAGKPA
                     AGPPNPA"
     gene            complement(14481..>15047)
                     /locus_tag="VGC69_02870"
     CDS             complement(14481..>15047)
                     /locus_tag="VGC69_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY0294260.1"
                     /translation="PTQTNSPATLAPNASWGSAAGLTATQNAPGSLLNTPGGASGNSA
                     TSLNLSSALGADNSNPLAGIHPDATTLVRQQLDVLANQALAWQGQAWPGVEMEWEVER
                     DRSQGGEIIDDTHWATRLQLELPRLGLVQARLNLSGNQVILSLVAPSSAGELNQNSEA
                     LRHRFAAAGLTLSQLSVDAQPPSPFDLP"
BASE COUNT         2329 a         4472 c         5658 g         2588 t
ORIGIN      
        1 aaaagaaaaa gttgatagag ggatatggtt atgcgcgtca atctgccggt aaacgacaac
       61 gaagtacgga tgcgggagga tcagttcctg atctcgcgca cggataccaa ggggcgcatc
      121 atctatgcca atccggcgtt cgtggaaatg agcggctaca gccgcgcaga gctggaaggc
      181 aaggcgcaca acatcgtgcg ccatccggac atgccgcccg aggcttttgc cgacctgtgg
      241 cgcacgctgg aaaaaggcaa tacctggtcc ggcctggtca agaaccgccg caaggacggc
      301 agcttctact gggtgtacgc cacggtgacg cccatttacg agaacggcga ggtggccgcc
      361 tattcgtcgg tgcgcatccg tcccaccgat gagcagatcg acgcggccga cgcgttctat
      421 gcaaagctga atgcgggcaa ggcgcgcggc tggcaggtcc gcaatggccg cccggcccgc
      481 acgggcgtgc tgggcgcgtt gagcggcctg tcgtttcctt tgcgcagggg catgcgggcg
      541 catatgttcc gcacggcgac gctggccacc gtggcggcgg gcgccatggg ctggtatggc
      601 ctgtcggcgc gctgggctgc gatgagcggc ggcgaggcct ggggggcggg cgccgtggcg
      661 gtcctggtcg cggcggcggt ggccatcacg caatggcgcc tgaaccgccg catcgcccgg
      721 tcgatgacgt cggccggcga tctggtgctg caggtggccg ccggcaacct gacgacgaag
      781 ataggttcga atgcgaacga ccaggccggc ggcctggcgc tggccctgga gatcatgcgc
      841 aagagcctca ccaatattgc gcaggacgtg catgccggca tcgcccaggc cgtgcaggcg
      901 gccagcggca tcgccacggg caaccagttg ctggccacgc gcaccagcga cggcagcgcg
      961 gcgctgcagg ggacggcggc cagcatggaa gagatcactt ccacggtgaa gcagaacgcc
     1021 gacaacgcgc gccaggccag catcctggcc gatcgcagca tggacgtggc gcggcgcggc
     1081 ggctcggccg tggcgcaagt ggtcgacacc atgcacggca tctcgagcag ctcgagcaag
     1141 atcggcgata tcgtcaccct gatcgaaggc atcgccttcc agaccaatat cctggcgctc
     1201 aacgccgccg tcgaggcggc tcgcgccggc gaggcgggca agggctttgc cgtggtggcc
     1261 ggggaagtgc gttcgctggc gcagaagagc gcgcaggcgg cccgcgagat caagggcttg
     1321 atcgaagcct cggtcgaccg cgtcaacgaa ggatccatgc aggtcgaaca ggccggcgac
     1381 accatgcagg aaatcgtcga cgccgtgcag cgggtgtcgg acatcatcag cgagatctcg
     1441 gtggcttcgc agcagcaggc cagcggcatc gagcaggtgg acatggccgt gcagcagatg
     1501 gacggcatga cgcaggaaaa cctgagcctg gtgcaacagc tgggcgaggc ggtcgggcag
     1561 ttgaacgacc agtccgccga cctggatcag agcatccggg tgtttcgcct gcagccgggg
     1621 cagcactgag cgccggccgg gctagcgtcc cgccagcccg gtgagcaggc gtcccagcgt
     1681 gtcgtagccc tgctggaaca gggcttcgaa gtaggcgccg gagtgcggca ggaccgaggc
     1741 caagagcagc agtccggaaa tcaggctgat gggaaagccc accgtgaaca cggtcagttg
     1801 cggggccgcg cggttgagca cgcccatggc caggttcgtg atgagcaggg cgcagatcag
     1861 cggcagggcc agcagcaggc cggacgaaaa aatgctcgct ccccattcgg ccaggatgcc
     1921 ccagccgttg cggttgagcg cgtcgaagcc gaccggcagg gtgtcgaagc tgtgcgccag
     1981 cgcggccagc agcagcaggt ggccgtcaaa gctcagaaac agcatggtgg cgatgatgtt
     2041 gaggaactgc ggcagcaccg aggttgtggc gccttgcatc gggtcgaaaa acgaggcgaa
     2101 ggacaggccc atttgcaggc tgatgaactc cccgccggtt tgcacggcgg caaagacgac
     2161 gcgcatgacc aggcccatgg ccaggccgat gacggtttgc tgcatgacca ccatgatcgc
     2221 ctcgtaagag ttcaggtcga tcgcgggcat ggggccggcg gccggggcga tggccagcgt
     2281 cagcatgaag gacaggccga tcttgacgag ggtggggatg cccgactcgg aaaaaatggg
     2341 cgaggcgatg atcagtccca gcacgcgcac gaacggccag aggaattggc ctatccagcc
     2401 gttgagctgg tccatggtga aggtgatcat ggcgcgcggg cgtgcggcgg gacgggttgc
     2461 cgcggctcag gagaccagcg agggaatctg ggtgattatc tggcgcatgt agtcgaccat
     2521 cagattgatc agccaggggc cgagcaggac gaggcccgcg cagatggcca gcagcttggg
     2581 aatgaacgac agcgtcattt cgttgatctg cgtggccgcc tggaacacgc tgatcaccag
     2641 gccgaccacc aggctgatga gcagcagcgg cgtggccatg gccagggaaa ttttcatcgc
     2701 ctggtaggcc agggtcatga cggtttcggc ggtcattgcg cggttccccc gacattcatt
     2761 gatagaagct ctgcgccagc gagcccagca gcaagtgcca gccgtcggcc agcacgaaca
     2821 gcatgagctt gaaaggcagg gacatggtgg cgggcggcac catcatcatg cccagcgcca
     2881 tcagcacgct ggccaccacc atgtcgatga tgagaaatgg aatgaagatg gtgaagccga
     2941 tctggaaggc ggttttcagc tcgctggtga tgaaggccgg gaccaggatg cgcatgggca
     3001 cctgggtcgg gtcgctcatc gccggctgct tggccatgtc ggcgaacagg gaaagatcgt
     3061 tctcgcgcgt ctggtgcagc atgaaggtgc gcagcggctg gctggcccgg tctacggccg
     3121 tttcgaacga gatggcgttg ttcgacaggg gctggtaggc gtcggtgtag attttgtcga
     3181 acaccggcga catggtgtag aaggtcagga acagggccag gccgatcagc acattgttgg
     3241 gcggcgacat ctgcgtgccc atcgccgtgc gcagcaggcc cagtacgatg atgatgcggg
     3301 taaagcccgt catcatcagc agggccgccg gcaggaaggt cagcgaggtc atcagcagca
     3361 tggtctgcat gctgagcgaa tacgtttgcg agccgccggg accgggcgtg gccgtcaggg
     3421 cgggcagggt agcctgggcc aggctgctgc ccgggaagag ggccaggccg atgagcgcca
     3481 tgagggcgta gcggagccag gccgggcgcc gatgcgggct cgacggcgac gcattcgggc
     3541 cggagcgttg gaaaacggac gacatgacgc gcgcgcggga atggttcaag ggtgggctca
     3601 aggacgcttg agcactttgc ccagcttggc ggccagcgtc tgggcaaagg cttcgccggt
     3661 agcgggctcg ggggctgcgg gcgcggcttg cgcgggcagg gtatgcaggg cggtgatgct
     3721 ggtgggggtc aggcccagca ccagccaggt gtcttgcacc tggatcacgg ccacgtgctg
     3781 gcgcggaccc agcgacaggc gggaaaccat ccggaggctg ctggcgtcct ggcggggaat
     3841 gaaaccggcg cgccgcgcca gccaggccat ggccaggatg accgccacga ccagcacgag
     3901 gacgatcgtg acgcgcagga cggcgtcttg catcatgccg tcaacgccgg ttgttgagcc
     3961 ggttcaggcg ttctgacggc gtgatgatgt cggtcaggcg gatgccgtat ttgtcttcga
     4021 ccacgaccac ttcgccctgg gcgatgagat agccgttgac gaagatgtcc atcggttcgc
     4081 cggccagcgc atcgagctcg acgatggaac cctggcccag ttgcagcagg ttcttgatgg
     4141 tcaggcgcgt gcggcccagc tcgaccgtga gctggacggg aacgtccatg atcaggtcga
     4201 tgtcggtggt gctggcggcc ccttcgttgc tgatgagcgg cttgaagacc gaggtggcgg
     4261 gctgtgccgt cgacgccgcg gtggctgcgg cggcgggagc aggctcggcg gcggcttcgg
     4321 ctgccgtgcg caggccgtcg gcctgcgtgg gcggcaccgt gcgggcctgc tcggcgaggg
     4381 cctcggccca gtcgtcggtt gcggaagcgg cgggtggggt ggcgccgggt tgagcgggcc
     4441 ggtcgttttg atcagccatg tttgaaggcc tcgttgaaat cggagtcgtc gtggtggatc
     4501 atgttttgta cgcgcagggc atactggcta ttgaaagcgc cgtagccgca ttccatgagc
     4561 ggtacgccgt tgacatgggc ggtgatggat tcgggaatct cgacaggcag cacatcgccg
     4621 accttcatgg ccagcagttc gcgcacgctg gacggaatgc gaacgaattc cgcgaccagg
     4681 tcgatgtcgg cgctgcgcac ctggcgggag agctggtggg tccagcgctg gtcgatttct
     4741 tcgagcgcgg tctcctgcat cgggcgggcg aggatgtcgc ggatgggctc gatcatcgaa
     4801 tagggcaggc agatgttcag gtcgccgccc tgcgcgccga attcgatgtg gaagctggaa
     4861 atcaccacca cttcgttgcc gccggtgatg ttggcgaact tggtgtgggt ttcggagcgc
     4921 acgtattcga actcgatcgg atagaccgaa tcccaggcgc ggccgtagct gtccagggtg
     4981 aggttgagca ggcgccggat gatgcgctgc tcggtgttgg tgaaatcgcg gccttccacg
     5041 cgcatgtggt agcggccgtc gccgccgaac aggctgtcga ccaccaggaa caccaggttg
     5101 gcgtcgtagg ccagcatggc cgtgccgcgc agcggccgca tctgcacgat gttgaggttg
     5161 ctgggcaccg ggatgttgcg ctcgaaatcg gcgtacttct cgatgcggat cgagctgacc
     5221 gtgatgtcgg cgctgcggcg catgaagttc agcagcacgg tacgcatatg gcgcgcgaag
     5281 cgctcattga tgagttccag cgtctgcatg cgccgccgca cgacgcggtc gggcgagctc
     5341 aggtcgtaag cgcgggtgcc aggcgggtcg ttgttgggga ccggactgct gtcttgcggc
     5401 tcgccggtga cgccggcgag cagggcatcg acttcgtcct gggaaagaaa cgcctcatag
     5461 gccatgctta ttgcaccacg aattccgtga agagaacgtc ggagacgttc tgcccatcgg
     5521 ggatgggcgc gaaaggcttg ctgacggcgg cggccacgga cttggtgatc ccggccttgc
     5581 cttccggcgt gcggatgtct tgcggcgaaa ggtcggacag caccatgagg atgcggctgc
     5641 ggacttcggg caggtatttc tcgatgcggg cgcgcgactg ttcgtcgccc aggcgcagcg
     5701 tgagcgccac atgcagcagg cgttccgagt ccttgtcttc gagcatgacc gtgaacgggt
     5761 ccagcggcat gaagatgggc gcgggaatct gggtgacggc gggcggcgcg gtataggaga
     5821 cctgcgtggg cgtcgaggac tggccgacgg agagctggac gggattggtt tcgcggctgg
     5881 actgccagtg caggtagaac caggtgccgg caatgcacag ggcggccacg atgatcagaa
     5941 ccagcagcca gatcagcgga cgcaggaggc cgccgaaacc caggccggag gaggaggaaa
     6001 cgctgcccag cttggtgggg gatttgattg tcgtcgccat cgggttatag gggcgtgggt
     6061 gtacggcgga aactgaaccg atactgatgg aatggaagca ttctgcccga aaccttgcat
     6121 gccaaggcac gcgaaaaaca ggtcgaaagc cgggtatctc aggctattac ggcacgaatt
     6181 cccttaagca aaagtgtcga ccagcgcgtt ggcgtcgcgc ggcgcggcaa cctggagcac
     6241 ggcggagacg ctaccgccgc ccgccggctc cgaggcggcg aagcccccgc cggaccggcc
     6301 gcgcgcggct tgttcctggc cggactgcgc gccctgttcg ccgacgctgg tctggcccag
     6361 cgacaggccg gcctgctcga aagcgtgctg cagctgcggc atggccgatt ccagcgcctg
     6421 gcgcacggcg gcatgggccg agacgaaggc ggcgctggca acgccgtcgc tcaggttgat
     6481 cgtgacgcgc agaggaccaa ggtcgggcgg atccaggcgc agttcggcgg tctggccgcc
     6541 gcgctgcgtg ccgtggctga gcaggaccat ctggcggccg agatcgaggt tccactgggc
     6601 cgagccaacc ggcgaggaga ccagattgat cgaaatcgga gccggggtga gggcgtagcc
     6661 cgccgcggtc gtttgcgtgg cggcagccgc ggggacagcc gtcatggcgg ccaggctggc
     6721 ctgggcgtcg gtattgccgg cggcgtgcgg caggggcatg gccggttgct gcgcggactg
     6781 ctgggcctgg agctgctgca gggcgtcggc cgtgggcgtg ccggcatcgc cggcctgggc
     6841 gcccgcgtgg gcggcgtgcg cggtctggcc ggcaggcgtg gcggcagcca ggctggtgtc
     6901 cgttgccgcg gcctggcggg ccatctgggc aggcgtttgc gctatggttg cggcaggctg
     6961 ggccggttgt tgcgcggcgt cggccagcgg atccagcgtg gccgagtcgc gggtttgcga
     7021 tgtcggcgtg gaggcgtgca gggccggcgt ttgcagggct gtcgaggcgg caggcttggc
     7081 ggccgacgtg gccgatgcgg ttgacgcggt ggcgaggacg ggcagggtgc cgtgtttctc
     7141 ggtcgcggcc ggggcgggcg cggccgactg gcggttggcg acctggcttt gcacggcggc
     7201 cacctgcgcg gcgatgtcca gcgcctgctg cggcaggctg ggcgcggcgc tttgcatggc
     7261 ttgctgcggg tcggcggcag gctgctgggg cagggcctgg accgcggcgg tgttggtcag
     7321 cgcagtgctt gcggcgtcgg ccggatcggc cggatcgtcc ttgccgttgc cgtgcttggc
     7381 ggaggtgttg ccggccgttt tatccgtggc cgaaccagtg tcgccgtcgc cggcctgggc
     7441 ggcaggcctg ccgttgccgg cattgtcggc gttgctggaa tggtcggccg attgcgcggg
     7501 cggcgccttg ctgccgggcg cctgattcgc gccgttgccg gcttgcccgg ccagcacttg
     7561 cgagaagctg gggttgttgc cattggcgcc gcaagccggg gcggcttgca cggcggcggc
     7621 ctgtgccggt tgcggcgcgg cggcattcag ggcggcgagg ttcagggcgg tggtcatgga
     7681 cgttcccgtg gcgaggcgct tgattcagta gaaggaatgc cgctggctgg ccagccgcgc
     7741 cgaatgctcg tcgctcgcgc gctgttcgcg acgctccgcg acgacctgtg ccgcgcgggc
     7801 ttcgcgctgc gccaaggtgt cgaaggcatt cagtttgcgt tgctgctgct gccagtgcaa
     7861 gcggcttttc tcatgattga cattcagcgt atcgagcgcg gcgacctgct ggttgatggc
     7921 atcgtcgagc gtgctgatga agcgctggta attgtggcaa tcggaggtgg acatgcccgt
     7981 ctgcatggct tgctgcaggc gcaccaggta gtcgtggcga tagtcctgca gcatgccgag
     8041 ctgatgttcg gcgttttggc gttcgcggtt caggcggccc agggagcggg cggcctcgtc
     8101 ggcgtgctcc ctggccaggc cgatcagcgt ttcgagaggc agttggctag gcatggatgt
     8161 ctcgcggttc gatgatggcg cgcagctggg cgatggtggg ttcgtagccg acgttctcgc
     8221 cgacgtcctg ctgcaggaag gcttccaggc gcggatagcg cgcgatggcg tcgtcgagct
     8281 gcggatcgtt gccggccgcg taggcgccca cggcgatcag gtcgcggttg cgctggtagc
     8341 gggagatcga ctgcttgaag cgtcgcacca ggttgaactg ctgaggcgtg atgagcgagg
     8401 tcatggcccg cgagatcgag gcttcgatgt cgatggccgg atagtggccg gcttcggcca
     8461 gatagcgcga cagcaccaca tggccgtcga ggatggcgcg cgcggagtcg gcgatcgggt
     8521 cctgctggtc gtcgccttcg gccagcacgg tgtagaaggc cgtgatcgac ccggccttgc
     8581 cgttggcgcc gggcgaaccc atgccggcgc gttcgaccag cgcgggcagg cgggcgaaca
     8641 ccgagggcgg atagcccttg gtggcgggcg gttcgccgat ggccagggcg atctcgcgct
     8701 gcgccatggc gtagcgggtc agcgagtcca tgatgagcag gacgtcgagc ccctggtcgc
     8761 ggaagtgttc ggccaggcgc gtggcatagc tcgcgccctg caggcgcagc aagggcgaga
     8821 cgtcggccgg cgcggccacc acgaccgagc gcgccaggcc ttcggggccc aggttgtgct
     8881 cgatgaattc cttgacttcg cggccccgtt cgccgatcag gcccaccacg atgacgtcgg
     8941 cgcgcgtgta gcgggccatc atgcccagca gcacgctctt gcccacgccg gagccggcga
     9001 acaggcccat gcgctggccg cggcctaccg tgagcaggcc gttgatggcg cgcacgccca
     9061 catccagcac gctgtcgatg ggcgcgcggg aaagcgggtt gatgggcagc gccgtcagcg
     9121 gcgcgagttc ggcgccgacc agcgggccca ggctgtcgag cgggcggccg gcgccgtcga
     9181 ccacgcggcc cagcagggca ttgcccacgg gcagatggcg gcccagttgc ggtttggcat
     9241 tgccgttgag ctcggcattt tttggcaggg gcacgcgccg ctgcacggcg ggctcggccg
     9301 gcaccacgcg ggcgccggga ggcaggccgg agatgtcggc ctgcggcatc aggtagagcg
     9361 tatggccgtc gaagcccacg acttcggcgt cggcccagtg gtcgtggccg cgggcgattt
     9421 cgatgcggca ggcggcgccg acgggcaggc gcaggccggt ggcgtgcagc accaggcccg
     9481 tggcgcggat gatgcggccg gtgaccagcc agggttcggt ggaggcggcg cggatcgagc
     9541 cgatttcgag ctgcgtcttc cagcgctcgg ccagcggcac cgatggcgcg tcggcgccgg
     9601 gccgtgcttc ggtctggacg ggcgcgttca tggctgcgag tcttcccatg cggcattgcg
     9661 gcccagcgag gccgcgacgc ggcgccagcg cgtgtgcagc gtggcatcga tgtcgccgta
     9721 gggcgtttcg gcgcggcagc cgccgcgttc gatggactcg tcggccagca cgcgccagtt
     9781 ggcttccttg agctcttcgg ccagctgcag gcgcaccagt tcgaggtcgt cggggtgcac
     9841 ccacaggcgc atcacgcctt gcggcacggc ggggttcagg tgcaacactt cacgcacggc
     9901 gtgcagcatg gattcgggat gctcggcgag ttcgccgcgc agcacctggg cggcgatgtc
     9961 cagcgccagg gtgatgaggc tttgccccat ttgctcttcc aggccgccca tcgccgcggc
    10021 ggtgcgggag accagcgtgt tcagtcggac ggcttcctgc ttgccttgtt cgcggccgtg
    10081 ggcgaggccc tcggcatggc cggcggcata gccttcggcg tggccagcct tgcggccttc
    10141 gttcaggccc tgcatatagc cttgcttgtg gccgtcggcc tggccctgtt ggtgggcctc
    10201 gcggtaggct tcctcgcgcg cggcctgcac gatttcctcg aggctggggc cggcgggttg
    10261 cggcgcgggg tcggcggcct cgggttcggc cgcggcttcc ggctcgtcga acgacatcat
    10321 ctcccagcgg cgccaggcgg cgcggtgcga cagcgggggt tgatgcggat cgacgggcag
    10381 gcggtcagac ataggcgtcg tccccttgcg cgcccagggt gatctggccg gcctcggcca
    10441 ggcggcgggc gatctggatg atcttcttct gttcggactc gaccttggac atgcgcaccg
    10501 ggccctgggc gtcgaggtcc tcgcgcagca tttcggcggc gcggctcgac atattgcgca
    10561 ggaacttgtt gcgcagctct tcgggcgcgc ccttgagcgc caccatgagc atttcgttgt
    10621 cgatttcctt gaggacgagc tggatgccgc ggtcctcgac ctcgagcagg ttctcgaagg
    10681 tgaacatctc gtcgacgatc ttctgggcca ggtcggcgtc gcgctcgcgc aggctgctga
    10741 ccacggcctc ttcctgcgac gagttcatca tgttgaggat ctcggccgcc gtgcgcacgc
    10801 cgcccatctt gctgcgcttg gcgctttggc cggccagcat ggaattgagc acttcggtga
    10861 gttcggccag cgcggtgggc tgcacgccgc cgaaggtggc gatgcgcagc atcacgtcgt
    10921 tgcgcaggcg gtcgctgagc atggccagca cgccggcggc cttgtcgcgc tcgagatgca
    10981 ccaggatggt ggcgatgatc tgcgggtgtt cgttgccgat gagctcggtg acggtggtgg
    11041 cgtcgagcca gttcagggcg tcgatgccgt tgccgctttc gccggcctcg aggatgtcct
    11101 cgatcaggcc ggcggcgcga tcgctgccca gcgccttggt cagcacgctg cggatgtagt
    11161 cgtccgaccc gacgctgacg gccaggaact gatcggcttc ctggcggaat tcttccagca
    11221 cattgctcac gtcgcggcgg gtgacctgct tgagcgaggc catggcgccg cccaccatct
    11281 gcacttcacg ggccgacagg tagcgaaaca cctgggcggc cgcgtcttca cccagcgtca
    11341 tcagcatgac ggcgctgcgg gtcagtccgt cgatggtctt gccgtcagga gttgtcatct
    11401 tggttgttca tccaggagcg cagcaccatg gcaacggcac gggggtcttt catcgccatg
    11461 tcgcgggcgc gttgcaggtt gtcttcgtag cggttgattt cgcgctcgcg ggccaccgtc
    11521 atggccgcgc gttcggcctc gaggcgttcg gctttggcga cctcggggtc gacctgcggg
    11581 aagaagtagt ccatcgccgc cggcttgatc ttgcggtaga gccacagcag gacggccgcg
    11641 aagatcagcc agccgatgat ggtcttggcc agggcgatca actcggggtc gcgccagaag
    11701 ggcacggtgg gctcggcgtc gttgaaggcg ctgttgacca ggttgagcga gtcgccgcga
    11761 ttgtccgaat agcccatggc ctccttgacg aggtcggtga gcttggcgag ctcttcgggc
    11821 ggcaggggtt ggggatccct gccgtccttg ccgggcaggt tgttgatgac cacggccacc
    11881 gacaggcgct tgagcacgcc caccggcgcc ttggtgtggc tgatggtgcg gtcgagctcg
    11941 tagttggtgg tggcgtcgtg ccggtcgttg gtcggcacgt tctgctgctg ggcggcggcc
    12001 tgttgggccg cggtttgctg ggcggcctgg gcggcttggg gattgaccgc gccgggctgg
    12061 cccggctggg ccggcgggtt gacgatgggc gcctgcgcat tgggcggcgc ctggttgctc
    12121 agcgcgccgg gcacgccctg ggccgcgttg gcgccggtct gcttcgaatc gctggtttgc
    12181 tggctgcgga tggaggcctg gccggggtcc tggttggggc ggtaggtttc ggaggtttcc
    12241 tcgtggcgcg agaaatcgac gtcggcgctg gcctgggcat gcacattgcc cggaccgacc
    12301 agcgggttga ggatggtgag gatgcgctcg acggtgcgct gttcgacttc gcgcacgaag
    12361 cgcagctggt cggcgtccat gccgcggcct tcgccctgcg gcgcggacag caggcggccg
    12421 ttctggtcga cgatggagac gttgtcggcg ttgagttctg gaacgctgga ggccaccagg
    12481 taggagatgg ccgacacttg cgcctggctc aggctgcggc cggggtagac gctgatcagc
    12541 accgaggcgg tgggcgcctg gcgctcgcgc acgaacaggg actggcgggg cagcgccagg
    12601 tgcacgcggg cgtgctgcac ggtgttcatg gcttcgatgg aacgggcgag ctcgccctcc
    12661 aggccgcgct ggtaattgat ttgctcggta aactggctgg ccccgaagcg ggcgttgtcc
    12721 atgatctcga agccgatcga accgccgcgc ggcagcccct ggctggccag ttgcatgcgg
    12781 gtgttgtaga cgcggtcggc gggcaccagc agcgccgtgc cgttgtcgtt gaacttgtag
    12841 ggcacgttca tctgccccag ggcggtcacg atggcgccgc cgtcgcgatc gtccaggttg
    12901 gagaacagga ctttgtaatc cgggtcgcgg ctccagagca cggcaacggc gatgagggcc
    12961 acgagcgctg ccgcggcgcc cagcaggatg ggcttgggca gggctttgat tctggcgaga
    13021 accggaaacc ttgccagaat cgtggtgccg agagcagcct gttgattcat cggcggctcc
    13081 cgcaagctgc ggggtgcaga attggatacg acatgggcgg caaggaagac ttgttacgca
    13141 tgtagcgtga ctttagattg ggagcctgga ttatggccgc cccccggagt ccgccaatcg
    13201 acgaaaacga gcggatttct ggtgttattt gtttcaactg tccccagtca tggaccagtc
    13261 atggacgcag tgctgttaca tgtgaactat ccggtgtttt ggcggctttt gcgggcaatg
    13321 gccgggcggt ggccacgtta cgctggcgac catcccattg acgtcagcaa ggagatccga
    13381 ccatggcggt ttcaggcgtg tccggtgtcg aaagcatgct gcagcagatg cgcgcgctgg
    13441 tcgaggcggc ccagaagggc gtgtccgacc cgtccgagtt gtcgccgtcc gtgccgtccc
    13501 agcaggcggt cagcttcgcc gacgaactcc atcagtcgat caagcgcatt tccgacgcgc
    13561 agaatgcctc cacggcccag gccaaggctt tcgagctggg cgatccccgc gtgtcgctca
    13621 atgacgtcat gatcgatatg caaaaggcca gcatcggttt ccagacggcg atgcaggtgc
    13681 gcaaccgcct ggtcgaggcc taccgcgaag tgtcgtcgat ctcgatgtaa cgccgttcag
    13741 ccgtccaggc tgaactgccg caggctttgc ggaaacggcg tgatcatcag caggctccgg
    13801 tcgctgaact gcaggactgt cgacgggccg gcgtgaaact gcatgcctgt cttgtggtcg
    13861 tggatccggc catgaaagcc cagggtcagc gcggccagcc tggccagttc ggcttcgttt
    13921 tccagcagat cggcgtgggc ctcgccaagt cgccagaaaa agagcgacga ccgcgcatcg
    13981 attcccagga tgcggacccc cagatccagc tcatccccgg tgttgcgcag ccgtatccgg
    14041 cattcgggat gcgccagagc cgccagggcg tggcggatat ccatggcctg gcgcagccag
    14101 gcgtcgggca aggggaactc ggaaggggca gcaggcacgg gggccaccga ccggtcaggc
    14161 agggttgggg gggcccgcgg caggcttgcc tgcctgagcc tggccgggca ggccgcgcga
    14221 ctccaggcgg tacagccagg ctagcagttc cgccacggcc agataaagct ggggcgggat
    14281 gtgggagtcc aggtcgacct gcatgagcag gccgacgagc tcggacgact cgtgcacgta
    14341 caggccgtgt tcgcgcgcgg cgcgcacgat ggtgtcggcc agcttgccgt agcccttggc
    14401 cacgacgcgg ggcgaggcgt cgtgttcgtc gtacgagacg gccacggccg ctcggcgggg
    14461 gtcgggatag ccaataccgc tcatggaagg tcgaaaggcg aagggggttg ggcgtcgacg
    14521 ctgagctggc tgagggtcag cccggccgcg gcgaaccgat ggcgcagcgc ctcgctgttc
    14581 tggttgagtt caccggcgct gctgggggcg accaggctca gtatcacctg attgcccgaa
    14641 agattcaggc gcgcctggac caggcccagg cgcggcagct cgagctgcag gcgggtggcc
    14701 cagtgcgtgt cgtcgatgat ctcgccgccc tggctgcgat cgcgctccac ctcccattcc
    14761 atctcgacgc cgggccaggc ctggccttgc caggccaggg cctggttggc cagcacatcg
    14821 agctgttgcc ggaccagcgt ggtggcgtcg ggatggatgc cggccagggg gttggaattg
    14881 tcggcgccca gcgcgctgga aaggttcagg ctggtggcgc tattgcccga cgcgccgccg
    14941 ggcgtgttca gcaggctgcc gggcgcattt tgcgtggcgg tcaggccggc ggcgctgccc
    15001 caggacgcat tgggcgcgag cgttgcgggg gagttagttt gcgtggg
//
LOCUS       DASWPO010000048        14742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2151941, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000048 DASWPO010000000
VERSION     DASWPO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14742
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151941"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..721)
                     /locus_tag="VGC69_02875"
     CDS             complement(53..721)
                     /locus_tag="VGC69_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0294261.1"
                     /translation="MGFLGVLMLDTRFPRPPGDIGNPATFAALDIPVRYVTVRGASPV
                     RVIREGDPALLAPFIAAARTLAGQGAALISTSCGFLAGFQPDLAGALDVPVVASSLLQ
                     CAGLQAPGVLTIDAQSLTPALLARAGVPAGTPVQGIRPGSEFHTRLLFNHTQLDLDEA
                     RRNVVEGAQELVRSHPGVRSIVLECTNMPPYREAIEQATGRPVHDIVTLLRAHWRELR
                     RSGP"
     gene            810..1646
                     /locus_tag="VGC69_02880"
     CDS             810..1646
                     /locus_tag="VGC69_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294262.1"
                     /translation="MRFMHSIVKAVAAVSLAAAGFTAAAQSAGETGWEQVQRTKVLRV
                     GVIADAIPYFHKDLATGKWDGFGPDFADSLGKSLGAKVQFVETTWGNAVLDLQSNKID
                     VMFGLAPTPERAKAVDFSDTLFVNTYTSVCNKANAGKSWADLNNPNVKISVDVGSSND
                     MAATKMAPNANIQRFDSQGAATLALQSGRVDCQVLVILLAQPMLVKVPDAGTLTIPEP
                     FFSNATSIGLRKESDPKLREAVNKWLADARSRDEIKSVILSNMQKLVGVKPESFPANV
                     KF"
     gene            1660..2334
                     /locus_tag="VGC69_02885"
     CDS             1660..2334
                     /locus_tag="VGC69_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0294263.1"
                     /translation="MAGYTWHFDVLWQYRAILLSGLGYTILFTIICVALGLLVGLLTG
                     MGRLARSAWITAPIRAYIEVFRCTPVLVQLIWFYYALPVLAGISISAPVAAALCLSLY
                     GGAFYSEIIRGGIIATDAGQTEAGQALGMTRAQVMRRIVLPQAFKRMVPPLINQSIMQ
                     LKNTSLLSVLALPDLLYQGQVIAHDSYRPLEIYTLVAVLYFVVLLPVTIWAKRLEFGG
                     STAKEL"
     gene            2334..3104
                     /locus_tag="VGC69_02890"
     CDS             2334..3104
                     /locus_tag="VGC69_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0294264.1"
                     /translation="MTQVLIEIRDLEKSFGRHAVLKGISLEVAKGDVVALIGPSGSGK
                     STLLRCINLLTLPDAGKVRVGEHHAAFGQNATRLPGDRDLANFRARTGMVFQHFNLFP
                     HMTALGNVMEGPITVLKQKKAQAEATARELLQKVGLADKADVYPDKLSGGQKQRVAIA
                     RALAMRPEVMLFDEATSALDPELVGEVLQVIKSLAREGMTMILVTHEIAFAREVADQV
                     IFMRDGVVVECGPPSQVIEAPRQEATRSFLARFKEAHA"
     gene            complement(3116..4282)
                     /locus_tag="VGC69_02895"
     CDS             complement(3116..4282)
                     /locus_tag="VGC69_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0294265.1"
                     /translation="MDSSMNASPKIAIVGAGPGGLALARLLHLQGMDAAVFEREAHPL
                     ERPQGGTLDLHPESGLLALRRAGLADAFQAIARYEDQGTRLLDPCAHGLFEEQGAAHG
                     NRPEVDRTALRAMLLDALPRGMVRWNRPLRGIRDHGDGRWVLAFDAVPEGPFDLVIGA
                     DGAWSRVRPLLSPYQPQYSGLCFVEFRIDDVDRSHPGLARLVGRGKMHVEGGGKAIIV
                     QRNGNSHFCGYAIFRVPLGWVERRFDVTSPKAIREGLLREYAGYADNLLDLFRASNDH
                     FAIRPIHALPVGHCWTHRQGLTLIGDAAHVMSPFGGEGVNNAMLDAAELAQSLAACPQ
                     WDDAVSEYETHMFARVAESAEGAAEGAATFLSHDGQALTLDIYRSHGAMPRDPR"
     gene            4324..4791
                     /locus_tag="VGC69_02900"
     CDS             4324..4791
                     /locus_tag="VGC69_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0294266.1"
                     /translation="MSTEAESGDAALPMRLLGQATRTFARLVDAPLRQLGFAMSQLPV
                     LVTLKTAGALSQAELARMAQVEQSSMAQLLNRMERDGLVKRGPDPADGRSRLISLTAR
                     ACRQMPDGKSVMDKACETALTGFSTAERTQLRKLLLRIDANLERALAERDRER"
     gene            complement(4811..5971)
                     /locus_tag="VGC69_02905"
     CDS             complement(4811..5971)
                     /locus_tag="VGC69_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY0294267.1"
                     /translation="MTAAASTPTPGLAPYACDPAFTRGRLRAEPASGNRSEFQRDRDR
                     IVHSTAFRRLEYKTQVFVNHEGDLFRTRLTHSLEVAQIARTVARSLGLSEDLIEAIAL
                     AHDLGHTPFGHAGQDELNACMRELAPAAGGFEHNLQSLRVVDELEERYAEFNGLNLCF
                     ETREGILKHCSVAHARQLGDVGRRFLERTQPSLEAQLANLADEVAYNNHDIDDGIRSG
                     LISVEQMQDVAIFARHHAEVVKQYPQLVPRRAVSETIRRMINTLIVDLTATTRERIAA
                     HAPASADDVRRAPPLAGFSDAVRREADALKTFLFDNLYRHYQVMRMTAKMRRVVRELF
                     TAFLDDPRLLPPDYHRGEETAQARAIADYIAGMTDRYAIREHRRLFEINPQA"
     gene            complement(5985..7088)
                     /gene="aroB"
                     /locus_tag="VGC69_02910"
     CDS             complement(5985..7088)
                     /gene="aroB"
                     /locus_tag="VGC69_02910"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY0294268.1"
                     /translation="MTAVVHVDTPGGRYPIHIGAGRLDLLDASIPADATSIALITNPT
                     VGALYAARAQAALARTGRRVILVELPDGEVHKDWQTLNLIFDALLENRCDRRCVLVAL
                     GGGVIGDMAGFAAAVYMRGVRFVQVPTTLLAQVDSSVGGKTAVNHPLGKNMVGAFHQP
                     LAVEIDTEVLATLPAREISAGLAEVIKYGLILDPEFWTWCEAHAARLRALDREALSYA
                     IRRSCELKAQVVGKDERESGLRAILNLGHTFGHAIESGLGYGEWLHGEAVGCGLVQAA
                     ELSARVTGFAPADVARVRELVAAIGCPVTAPDLGAQRWLALMQVDKKTEGGEIKFVLL
                     PRIGEAVSRAAPEAALRDTLAQTTAHATAATAG"
     gene            complement(7085..7741)
                     /locus_tag="VGC69_02915"
     CDS             complement(7085..7741)
                     /locus_tag="VGC69_02915"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0294269.1"
                     /translation="MNRQANPRPLPDEPEVSSETEPSASAALAEPLGPCAANQPACLA
                     DGRPLFLVGMMGAGKTTIGRGLARILGREFVDLDHQLEARCGVRVPVIFEIEGEAGFR
                     RREAQALCECTQRRGIVLATGGGAILDPENRRLLHERGLVVYLRASLEELFRRTSRDR
                     NRPLLATEDPRGTLRQLMTARDPLYAEAAHLVVDTGTCSVNIVVKQLLSRLYNTEIAS
                     "
     gene            7857..10268
                     /locus_tag="VGC69_02920"
     CDS             7857..10268
                     /locus_tag="VGC69_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0294270.1"
                     /translation="MSTRQNSSKQDPQPASGGSWILRFFLKTAIFFGGLGLCVVLLAA
                     MGMALAWPNLPDLHAMTDYRPRVPLRVYTADNVLIGEFGEERRNVLRFSEIPDLMKSA
                     VLSAEDDRFYQHGGVDWAGVIRAAGTNLVSGSKAQGASTITMQVARNFYLSSEKTYSR
                     KFYELLLTFKIESQLSKDQILELYMNQIYLGHRAYGFSAAARTYFGKQLADLTPAEAA
                     MLAGIPKAPSRYNPISNMARAEVRQHYVLGRMLALGYLTEPEYKTAMDQKLALRSNEN
                     SPTGTYAVHGEYVAELARQLLYSVYQDNLYSRGINIYTTVRSKDQDLAYRSLRDGVLE
                     YTRRSAYPGPEDNIDLPADAERNPQALDDTIDEVIDKYPDSGGDMFTGVVLSASPTEI
                     RVARSSKEIITVNDKKALLTVARALGDKAKSDVRIKRGSVVYVHKNPDGAWEVINYPA
                     VQAAFVTLTPNDGAIRAMVGGFDFYRGNFNRVTQAWRQPGSNIKPFIYASALERGLSP
                     ATQISNEPFYLSAEQTGSKPWAPKNYGDEYNPMLTMRQALYKSMNLVTVRIMQAVGPA
                     YAQSYLMRFGFDKSRQPAVLPMALGAGTVTPLQLAGAYSVFANGGYRVTPFLIDHVTD
                     SNGKPLMQAKPTQAGDESIRAIDPRTAYVMNDLLQGVATYGTAARAHAVLKRDVAGKT
                     GTTNDSNDAWFSGYTSGLEGTAWLGYDQPRSLGAKETGGGVALPIWLGYMQEMIKTLP
                     EQPPRPRPDGLIVDNGELYFSEYPPGQAVASLGLGSDAQQAAAQQDTLGDFLSGLTGG
                     GSKAN"
     gene            complement(10349..10678)
                     /gene="cyaY"
                     /locus_tag="VGC69_02925"
     CDS             complement(10349..10678)
                     /gene="cyaY"
                     /locus_tag="VGC69_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEY0294271.1"
                     /translation="MTETEFLALVDQVLDSIESQADDWAATTDLDIETSRSGHVLTLV
                     FDNSIHIVINSQAPMQEIWVAARSGGFHYRYDGRGWNDTRGGPALPEALSQICSSAAD
                     TPLTVRL"
     gene            10711..10998
                     /locus_tag="VGC69_02930"
     CDS             10711..10998
                     /locus_tag="VGC69_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEY0294272.1"
                     /translation="MHLVRLVRSRPIPRIVAMLAAAGALAAGVAACGYKGPLYMPGSP
                     QDVHRRQPPPALKPGETPSEAATPDTAAPAPQPDTAGDAAKRTNPVPNLFQ"
     gene            10998..12291
                     /gene="lysA"
                     /locus_tag="VGC69_02935"
                     /pseudo
     CDS             10998..12291
                     /gene="lysA"
                     /locus_tag="VGC69_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937352.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
     gene            complement(12360..12866)
                     /locus_tag="VGC69_02940"
     CDS             complement(12360..12866)
                     /locus_tag="VGC69_02940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0294273.1"
                     /translation="MPSTPSFDSAHFRAALGRFATGVTIITTQAADGSPIGMAASSFN
                     SVSLEPPLVLWSLGLQASSYPAMTQCERYVIHVLAADHVALARRFATGKQPERFADLP
                     LATAPNGTLMLADESAAWFECRNRSQYQEGDHLIMVGEVEHCGYNAAAPLIFHAGGFA
                     LTPAHNPQ"
     gene            12946..13953
                     /locus_tag="VGC69_02945"
     CDS             12946..13953
                     /locus_tag="VGC69_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEY0294274.1"
                     /translation="MDTHSPQAATSSFAEIPLIDLAPLKDGRQASRSAVAAEICKACE
                     SVGFLYLANHGIAASLMDDMFGVARQFFALPLEEKMPLKLGAFSGFRGYLPAGVEGGT
                     MAGNRKEVFQIINEQADDGAGTLDPMWTQSNLWPASFPTLRKVMLQYLADVERLSLTL
                     LQLFAIGLEVPETTFSSRFNSPINMLRLLHYPPQAPTDKAIGSQPHTDSGALTILAQD
                     DAGGLESVDDLGQWSQVKPIKNTLVVNLGEMLKLWSDGRFRATPHRVVNASGTDRISI
                     PFFASPDIPTLIQPVIRAQTRKDEIQLVGHLERGTPKTSGEIMSNSWHRLWGGAKPAS
                     Y"
     gene            complement(14083..>14742)
                     /locus_tag="VGC69_02950"
     CDS             complement(14083..>14742)
                     /locus_tag="VGC69_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0294275.1"
                     /translation="TLATNLVYRKDKFPNGGPQSWADFWDVKKFPGPRSLPASMANRV
                     LAYALMADGVPHDKLYPLDVERAFKKLDEIKPHIDVWWSQGSQAEQLIRDGEVSMIAL
                     WNNRAVMMQQQNVPLELVWNDALVSDVVYGMVKGAKNAPLAWEFINYLADAKRQAGFS
                     SYLFYGAGNPEAYKLMTPDVLAALPGSDVHAKMVAFANSEWESVNGSKVTERFTNWRA
                     G"
BASE COUNT         2503 a         5049 c         4867 g         2323 t
ORIGIN      
        1 ccatgcatac aacctattga atcttcacac ctaggccgcg ggccccgccg cgtcagggcc
       61 cgctgcggcg cagttcccgc caatgcgccc gcagcagcgt gacgatgtca tgcaccggcc
      121 ggccggtggc ctgctcgatg gcctcgcggt agggcggcat attggtgcac tccagcacga
      181 tgctgcgcac gccgggatgg ctgcgcacca gttcctgcgc cccttccacc acattgcgcc
      241 gggcctcgtc caggtcgagc tgcgtgtggt tgaacagcag gcgggtgtgg aactcgctgc
      301 cgggccggat gccctgcacc ggcgtgcccg ccggcacccc ggcgcgcgcc agcagcgcgg
      361 gcgtcaggga ctgcgcgtcg atggtcagca cgcccggcgc ttgcaggccg gcgcactgca
      421 gcaggctgga ggccaccacc ggcacgtcca gcgctcccgc caggtcgggc tgaaagcccg
      481 ccaggaagcc gcagctcgtg gagatcagcg ccgcgccctg gccggccagc gtgcgcgcgg
      541 ccgcgatgaa gggcgccagc agcgccggat cgccctcgcg gatgacccgc acgggcgagg
      601 cgcccctcac cgtgacgtag cgcacgggaa tgtccagcgc ggcgaacgtc gccggattgc
      661 cgatgtcgcc gggcgggcgc ggaaagcgcg tgtccagcat caggacgccc agaaagccca
      721 taaattcatt ccaaggtcta aaatcgcgcc ttcgggcaaa atattgcgcc ccgcaagccg
      781 cgccaaaatc catactacag gggaattcca tgcgattcat gcacagcatc gtcaaggccg
      841 tcgccgccgt ttccttggcc gccgccggtt tcaccgccgc ggcgcaaagc gccggggaaa
      901 ccggctggga acaggtgcag cgcaccaagg tactgcgcgt cggcgtgatc gccgatgcca
      961 ttccgtactt ccacaaggat ctggccaccg gcaagtggga cggcttcggc cccgacttcg
     1021 ccgacagcct gggcaagtcg ctgggcgcca aggtccagtt cgtcgagacc acctggggca
     1081 atgccgtgct cgacctgcag tcgaacaaga tcgacgtgat gttcggcctg gcgcccaccc
     1141 cggagcgcgc caaggcggtc gacttctccg acacgctgtt cgtcaacacc tatacctcgg
     1201 tgtgcaacaa ggccaacgcc ggcaagagct gggccgacct caacaacccc aacgtgaaga
     1261 tcagcgtgga cgtcggctcc agcaacgaca tggccgccac caagatggcg cccaacgcca
     1321 atatccagcg cttcgacagc cagggcgcgg ccacgctggc cctgcagtcc ggccgggtcg
     1381 actgccaggt gctggtgatc ctgctggccc agcccatgct ggtcaaggtg cccgacgccg
     1441 gcaccctcac cattcccgaa cccttcttct ccaacgccac ctccatcggc ctgcgcaagg
     1501 aatccgatcc caagctgcgc gaagcggtca acaagtggct ggccgacgcc cgcagccgcg
     1561 acgagatcaa gagtgtcatc ctgagcaata tgcagaagct cgtcggcgtg aagcccgagt
     1621 ccttcccggc caacgtgaag ttctaagcag gtcggcgcca tggcgggtta tacgtggcac
     1681 ttcgatgtgc tttggcagta tcgcgcgatc ctcctgagcg ggttgggcta cacgatactg
     1741 ttcaccatca tctgcgtggc gctgggcctg ctggtggggc tgctgacggg gatgggccgg
     1801 ctggcccgct cggcctggat caccgccccc atccgggcct acatcgaagt gttccgctgc
     1861 acgccggtgc tggtgcagct gatctggttc tactatgccc tgcccgtgct ggccggcatt
     1921 tcgatatcgg cgcccgtggc ggcggccctg tgcctgtcgc tctacggcgg cgcgttctat
     1981 tccgagatca tccgcggcgg catcatcgcc accgacgccg ggcagaccga agccggccag
     2041 gccctgggca tgacgcgcgc gcaggtgatg cggcgcatcg tgctgccgca ggctttcaag
     2101 cgcatggtgc cgccgctcat caaccagtcc atcatgcagt tgaagaacac gtcgctgctg
     2161 tccgtgctgg cgctgccgga cctgctctac cagggccagg tgatcgcgca cgactcctac
     2221 cgcccgctgg aaatctacac cttggtcgcc gtgctgtatt tcgtcgtgct gctgcccgtg
     2281 acgatctggg ccaagcgcct ggaattcggc ggttccacgg ccaaggaact gtaatgacgc
     2341 aagtcctcat cgagatccgc gatctcgaaa agtccttcgg ccggcacgcc gtcctgaaag
     2401 gcatcagcct cgaggtcgcc aagggcgacg tggtggcgct gatcgggccc tcgggctcgg
     2461 gcaagtccac cctcctgcgc tgcatcaacc tgctgaccct gcccgacgcg ggcaaggtgc
     2521 gggtgggcga acaccacgcc gccttcggcc agaacgccac ccgcctgccg ggcgaccgcg
     2581 atctggcgaa cttccgcgcc cgcacgggca tggtgttcca gcacttcaac ctgttcccgc
     2641 acatgaccgc gctgggcaat gtgatggaag gccccatcac cgtgctcaag cagaaaaagg
     2701 cgcaggccga ggccacggcg cgcgagctgc tgcaaaaggt gggcctggcc gacaaggccg
     2761 atgtctaccc cgacaagctc tcgggcgggc agaagcagcg cgtggccatc gcccgcgcgc
     2821 tggccatgcg ccccgaggtc atgctgttcg acgaggccac ctcggcgctc gaccccgaac
     2881 tggtgggcga agtgctgcag gtaatcaagt cgctggcccg cgagggcatg accatgatcc
     2941 tggtcacgca cgaaatcgcc ttcgcccggg aagtggccga ccaggtgatc ttcatgcgcg
     3001 acggcgtggt ggtggaatgc ggccctccgt cgcaagtgat cgaggcgccg cggcaggagg
     3061 ccacgcgcag cttcctggcg cgcttcaaag aagcgcatgc ctgatccttc gatggctagc
     3121 ggggatctcg gggcatcgcc ccatggctac ggtagatgtc gagcgtgagc gcctgaccgt
     3181 cgtgcgacag aaacgtcgcc gccccttcgg ccgcaccctc ggcggactcg gcgacgcggg
     3241 cgaacatatg cgtttcgtac tctgacacgg catcgtccca ttgcgggcat gcggccagcg
     3301 attgcgcaag ttcggccgca tcgagcatgg cgttgttcac gccctcgccg ccgaagggcg
     3361 acatgacatg cgccgcgtcg ccgatcaggg tgagaccctg gcggtgcgtc cagcagtgcc
     3421 ccacgggcaa ggcatggatc ggccgtatcg cgaaatgatc gttgctggca cggaacaggt
     3481 ccaggagatt gtcggcatat cccgcgtatt cgcgcagcaa gccctcgcgg atggctttcg
     3541 gcgacgtgac atcgaagcgt cgctccaccc agcccagcgg gacgcgaaag atggcatagc
     3601 cgcagaaatg cgagttgcca tttcgctgca cgatgattgc cttgccgccg ccttccacgt
     3661 gcatcttgcc gcggccgacc agccgcgcca atcccggatg ggaacggtcg acgtcatcga
     3721 tacggaattc gacaaagcaa aggccgctgt attggggctg gtacggcgac aacagcggac
     3781 gcacgcgcga ccaggcgcca tcggcgccga tcacgagatc gaaagggcct tcgggaaccg
     3841 catcgaacgc caggacccag cggccgtcgc catggtcccg aatcccgcgc agcggccggt
     3901 tccagcggac catcccgcgc ggcagcgcat cgagcagcat ggcgcgcaac gccgtccgat
     3961 cgacttcggg acgattgccg tgcgcggcgc cctgctcctc gaaaagccca tgcgcgcagg
     4021 ggtcgagcag ccgagtccct tgatcttcat aacgggcaat cgcctggaac gcgtccgcca
     4081 atccggcgcg gcgcagcgcc agcaggccgg attccgggtg caagtccagc gtgccgccct
     4141 gcggccgttc gagcgggtgc gcctcgcgct cgaataccgc ggcgtccatg ccctgaagat
     4201 gcaggaggcg ggccagcgcg agtccgccgg gacctgcgcc gacgatggcg atcttcgggc
     4261 ttgcattcat ggaggaatcc atcaaaatag gtacctatct aaagtatata ggtgcctatt
     4321 taaatgtcaa ccgaagcaga aagcggcgac gccgccctgc ccatgcggct gcttggccag
     4381 gcaacccgga catttgccag gctggtcgac gcgcccttgc gccaattggg gtttgcgatg
     4441 agccagctgc ccgtgctcgt gacgctgaag accgccggcg ccttgtccca ggcggagctg
     4501 gcgcgcatgg cgcaggtcga gcagtccagc atggcccagc tcttgaatcg catggagcgc
     4561 gacggcctgg tcaagcgcgg gcccgatcca gccgacggac gcagccggct gatctcgctg
     4621 acggcccgcg cttgcaggca gatgcccgac ggcaagtcgg tcatggacaa agcctgcgaa
     4681 acggccttga cgggattcag cacggcagaa cggacgcagt tgcgaaagct attgctgagg
     4741 atcgatgcca atctggaacg ggcgctcgcg gaaagagacc gcgagcgata gcccgggaat
     4801 caggcgcgac tcacgcttgc gggttgatct cgaacaagcg ccggtgctcg cggatcgcgt
     4861 agcggtccgt catgccggca atgtagtcgg cgatggcgcg cgcctgcgcg gtttcttcgc
     4921 cgcggtggta gtcgggcggc agcaggcgcg ggtcgtccag aaaggccgtg aacagctcgc
     4981 gcaccacgcg gcgcatcttg gccgtcatgc gcatgacctg gtaatgccgg tagaggttgt
     5041 cgaacaggaa cgtcttgagc gcatcggcct cgcggcgcac ggcatccgag aaaccggcca
     5101 gaggcggcgc gcggcgcacg tcgtcggcgc tggccggcgc atgcgccgca atgcgttcgc
     5161 gcgtggtggc cgtcaggtcg acgatgagcg tgttgatcat gcggcggatg gtttccgaca
     5221 cggcgcggcg cggcacgagc tgcgggtatt gcttgaccac ctcggcatgg tggcgggcga
     5281 agatcgccac gtcctgcatc tgctccacgg agatcaggcc cgagcggatg ccgtcgtcga
     5341 tgtcgtggtt gttgtaggcc acctcgtcgg ccaggttggc cagttgcgcc tccagcgagg
     5401 gctgggtgcg ctcgagaaag cggcggccca cgtcgcccag ctggcgcgca tgcgccaccg
     5461 aacagtgctt aagtatgcct tcgcgcgtct cgaagcacag gttcaggccg ttgaattcgg
     5521 catagcgctc ttcgagctcg tcgaccaccc gcaagctctg cagattatgt tcgaagccgc
     5581 cggcggccgg cgccagctcg cgcatgcagg cattgagctc gtcctgcccg gcatggccga
     5641 agggcgtgtg gcccaggtcg tgcgccagcg cgatggcctc gatcaggtct tccgagagcc
     5701 ccaggctgcg cgccacggtg cgggcgatct gcgccacttc caggctgtgc gtcagccgcg
     5761 tgcggaacag gtcgccttcg tggttgacga agacctgggt cttgtattcg agccggcgaa
     5821 aggcggtgga gtgaacgatg cggtcgcggt cgcgctggaa ttcgctgcgg ttgcccgatg
     5881 ccggctcggc gcgcaggcgg ccgcgcgtga aggcggggtc gcaggcatag ggcgccaggc
     5941 ctggggtcgg ggtcgatgcg gctgccgtca tgaccgctgc gtcctcagcc cgccgtagcg
     6001 gccgtggcat gggctgtcgt ctgcgccagc gtatcgcgca gcgccgcctc gggcgcggcg
     6061 cggctgacgg cctcgccgat gcgcggcagc agcacgaact tgatctcgcc gccctcggtc
     6121 ttcttgtcga cctgcatcag cgcaagccag cgctgcgccc ccaggtcggg cgcggtgacg
     6181 gggcagccga tggccgccac cagctcgcgc acgcgggcca cgtcggccgg cgcgaagccc
     6241 gtgacgcggg ccgacagttc ggcggcctgc accaggccgc agcccacggc ctcgccgtgc
     6301 agccactcgc catagcccag gccggactcg atggcatggc cgaaggtatg gcccaggttg
     6361 agaatggcgc gcagcccgga ctcgcgctcg tccttgccca ccacctgcgc cttgagctcg
     6421 caggaacggc ggatggcgta ggacagcgcc tcgcggtcga gcgcgcgcag cctggcggca
     6481 tgggcttcgc accaggtcca gaactcgggg tcgagtatca ggccgtactt gatgacctcg
     6541 gccaggccgg ccgagatctc gcgcgccggc agggtggcca gcacctcggt gtcgatctcc
     6601 accgccaggg gctgatggaa ggcgccgacc atgttcttgc ccagcgggtg gttcaccgcc
     6661 gtcttgccgc ccaccgagga gtccacctgc gcgagcagcg tggtgggcac ctgcacgaag
     6721 cgcacgccgc gcatgtagac ggcggccgcg aagcccgcca tgtcgccgat caccccgccg
     6781 cccagcgcca ccagcacgca gcgccggtcg cagcggtttt cgagcagggc gtcgaagatc
     6841 aggttgagcg tctgccagtc tttgtggacc tcgccgtcgg gcagttcgac gaggatcacg
     6901 cggcggccgg tgcgagccag ggcggcctgc gcgcgcgccg cgtacagcgc ccccacggtg
     6961 ggattggtga tcagggcgat ggaggtggcg tcggccggga tgctggcgtc gagcaggtcg
     7021 agccggccgg cgccgatatg gatgggatag cgcccgccgg gcgtatcgac gtgaaccacg
     7081 gccgtcatga tgcgatctcg gtgttgtaga gcctggacag cagctgcttg accacgatgt
     7141 tgaccgagca ggtgccggta tcgacgacca agtgggccgc ctcggcatag agcgggtcgc
     7201 gcgcggtcat cagctggcgc agggtgccgc gcgggtcttc ggtggccagc agcggccggt
     7261 tgcggtcgcg cgaggtgcgg cgaaacagtt cctcgagcga ggcgcgcagg tagaccacca
     7321 ggccgcgctc gtgcagcagg cggcggtttt ccgggtccag gatggcgccg ccgccggtgg
     7381 ccagtacgat gccgcgccgt tgggtacact cacacaaggc ctgggcttcg cgccggcgaa
     7441 atcccgcctc gccttcgatc tcgaaaatca cgggcacgcg cacgccgcaa cgcgcctcca
     7501 gttgatgatc cagatccacg aattcacggc ccagtatccg ggccagtccg cgcccgatgg
     7561 tggtcttgcc cgcgcccatc atgccgacca ggaacagcgg ccggccatcg gccaggcagg
     7621 cgggctggtt ggcggcgcac ggcccgaggg gctcggccag cgccgcagac gccgacggtt
     7681 cagtctcgga cgagacctca ggctcgtccg ggagcggacg ggggttcgct tgcctattca
     7741 tggcggcatt atcccattgc cgccagttgt gcgcttacca cagacggggc tatttgttac
     7801 agccctcggg cccgcttgcc cggaactaga ctgagataaa aagtaaaatc ttcgcgatga
     7861 gcacgcgcca gaattcctcc aagcaagacc cgcagcccgc atcggggggc tcctggatat
     7921 tgcggttttt cctcaagacg gccattttct tcggggggct cggcctgtgc gtcgtgctgc
     7981 tggccgccat gggcatggcc ctggcctggc ccaacctgcc cgacctgcac gccatgaccg
     8041 actaccggcc gcgcgtgccg ctgcgcgtgt atacggccga caacgtgctc atcggcgaat
     8101 tcggcgaaga acgccgcaac gtgctgcgtt tctccgaaat ccccgacctc atgaaatcgg
     8161 ccgtgctctc ggccgaggac gaccgcttct accagcacgg cggcgtggac tgggcgggcg
     8221 tgatccgcgc cgcgggcacc aacctggtca gcggctccaa ggcgcagggc gccagcacca
     8281 tcaccatgca ggtggcgcgc aatttctacc tgtcgtccga gaagacctac tcgcgcaagt
     8341 tctacgaact gctgctcacc ttcaagatcg aatcgcagct cagcaaggac cagatcctcg
     8401 agctgtacat gaaccagatc tacctgggcc accgcgccta cggcttctcg gccgcggcgc
     8461 gcacctattt cggcaagcag ctggccgacc tcacgccggc cgaggccgcc atgctggcgg
     8521 gcatccccaa ggcgccttcg cgctacaacc cgatctcgaa catggcccga gccgaagtgc
     8581 gccagcacta cgtgctgggc cgcatgctgg cgctgggcta cctgaccgag cccgaataca
     8641 agaccgcgat ggaccagaag ctggcgctgc gctcgaacga gaactcgccc accggcacct
     8701 acgcggtgca cggcgagtac gtggccgagc tggctcgcca gctgctctac agcgtctacc
     8761 aggacaatct gtattcgcgc ggcatcaaca tctacaccac cgtccggtcc aaggaccagg
     8821 atctcgccta ccgctcgctg cgcgacggcg tgctcgaata cacgcgccgc tcggcctacc
     8881 ccggcccgga agacaatatc gacctgcccg ccgacgccga gcgcaacccg caggcgctcg
     8941 acgacacgat cgacgaggtg atcgacaagt accccgacag cggcggcgac atgttcaccg
     9001 gcgtggtgct ttcggccagc cccaccgaaa tccgcgtggc gcgcagctcc aaggaaatca
     9061 tcaccgtcaa cgacaagaag gcgctgctca ccgtggcccg cgcgctgggc gacaaggcca
     9121 agtccgacgt gcgcatcaag cgcggctcgg tggtctacgt gcacaagaac cccgacggcg
     9181 cctgggaagt gatcaactat cccgccgtgc aggccgcctt cgtcacgctc acgcccaatg
     9241 acggcgccat ccgcgccatg gtgggcggct tcgacttcta ccgcggcaac ttcaaccgcg
     9301 tcacgcaggc ctggcgccag ccgggctcga acatcaagcc cttcatctat gcctcggcgc
     9361 tcgagcgcgg cctgtcgccg gccacgcaga tctccaacga gccgttctac ctgtcggccg
     9421 aacagacggg ttccaagccc tgggcgccca agaactatgg cgacgagtac aaccccatgc
     9481 tcaccatgcg ccaggcgctg tacaagtcca tgaacctggt cacggtgcgc atcatgcagg
     9541 ccgtcggccc ggcctacgcg cagagctacc tgatgcgctt cggcttcgac aagtcgcgcc
     9601 agccggccgt gctgcccatg gcgctgggcg cgggcacggt cacgccgctg cagctggcgg
     9661 gcgcctattc ggtgttcgcc aacggcggct accgcgtcac gcccttcctg atcgatcacg
     9721 tcaccgacag caacggcaag ccgctgatgc aggccaagcc cacccaggcc ggcgacgaat
     9781 ccatccgcgc catcgacccg cgcaccgcct acgtcatgaa cgacctgctg cagggcgtgg
     9841 ccacctacgg cacggccgcc cgcgcccacg ccgtgctcaa gcgcgacgtg gccggcaaga
     9901 caggcaccac caacgactcg aacgacgcct ggttctcggg ctacacctcg ggcctggaag
     9961 gcaccgcctg gctgggctac gaccagccgc gctcgctggg cgccaaggaa accggcggcg
    10021 gcgtggccct gccgatctgg ctgggctata tgcaggaaat gatcaagacc ctccccgagc
    10081 agcccccgcg cccacgcccc gacggcctga tcgtcgacaa cggcgagctg tatttctcgg
    10141 aatatccgcc cggccaggcc gtggcctcgc tgggcctggg cagcgacgcc cagcaagccg
    10201 cggcgcagca ggacaccctg ggcgacttcc tgagcggcct gaccggcggc ggcagcaagg
    10261 ccaattgacc ggttgaatat cgatcggtga atatcgatcg ctttgattcg acgcgcgccc
    10321 gccgggcccg ccaacggcgg gccgcgcttc agaggcgcac ggtcaggggc gtgtcggccg
    10381 ccgacgaaca gatctgcgac aaagcctcgg gcagggccgg gccgccgcgc gtgtcgttcc
    10441 agccgcggcc gtcatagcgg taatgaaagc cgccgctgcg cgccgccacc cagatttcct
    10501 gcatgggcgc ctgggaattg atcacgatat ggatgctgtt gtcgaaaacc agggtcagca
    10561 catggccgct gcggctggtt tcgatgtcga ggtcggtcgt ggccgcccaa tcgtcggcct
    10621 ggctctcgat gctgtcgagt acctggtcga ccagggcaag gaattcggtt tcggtcataa
    10681 tccaacctgc tatcgattac ggagtcttct gtgcatcttg tgcgcctggt acgcagccgc
    10741 cccatccccc gcattgtagc catgctggcc gccgccggcg cattggccgc cggcgtggcc
    10801 gcctgcggct acaagggccc gctctacatg ccgggcagcc cgcaagacgt ccaccggcgc
    10861 cagccgccgc ccgccctcaa gccgggcgaa accccgagcg aggccgccac gcccgacacc
    10921 gccgcgcccg cgccccagcc ggacactgcc ggcgacgccg ccaagcgcac caatcccgta
    10981 ccgaacctgt tccaataatg agcaccgcac ccgccgccca ggccgaattg gccggccacc
    11041 cgcatttcca ttaccgcgat ggcgtgctgc acgccgaggg cgtgccgctg gacaagctgg
    11101 cggccgagct gggcacgccg ctctacgtgt attcgcgcgc cgcgctgggc gccgcctgga
    11161 accgctacca ccaggccgcc ggcacccgac cggtgctggt ctgctatggc atgaaggcca
    11221 attccaacct ggccgtgctg cgcgaattcg cccgcctggg cgcgggcttc gacatcgtgt
    11281 cgggcggcga actggcgcgc gtgctggccg ccggcggcga cccgtccaag gtggtgttct
    11341 cgggcgtggg caagcaggcc tgggaaatgc gcgccgccat cgaggccgac gtgaagtgct
    11401 tcaacgtcga gtcgcaggac gagctctacc gcctctcgga cgtggcccgg ggcctgggcc
    11461 ggcgcgcccg cgtgtcgctg cgcgtgaacc ccgacgtcga cgcgcagacg cacccctaca
    11521 tttccaccgg gctgaaggaa aacaagttcg gcatcgccat cgaggccgcc cccgaggcct
    11581 accgcatcgc ggccgggctg ccgggcctgg aggtggtggg cgtggactgc cacatcggct
    11641 cgcagctcac cgacatcagc ccgtatttcg acgcgctgga aaagctcatc gacctgatcg
    11701 acacgctggc cgcctcgggc atcgccatcg cccacctgga cctgggcggc ggcctgggca
    11761 tccgctatac cgacgagatc ccgccctcgc cccaggcgct gctcgaccgc gtgttcgcgc
    11821 gcctgcaggc gcgcggctac ggccacctgc acctggtgct ggagccgggc cgctcgctgg
    11881 tgggcaatgc cgggctgctg ctgacgacgg tgcagttcct caagctggcg cccgcgcgca
    11941 acttcgccat cgtcgacgcc catgaacgac ctgctgcggc ccgcgctgta cgaggcctat
    12001 cacggcctgc gcgcggtgcg gccgcgcccg ggcgaggcgc gcgtgtacga cgtggtgggc
    12061 ccggtgtgcg aaagcggcga ctggctggcc aaggcgcgcg agctgtgcgt gcagcaggat
    12121 gacgtgctgg cgctggaatc ggccggagcc tacggcatga cgatggccag caactacaac
    12181 tcgcgtccgc ggcccgcgga agtgatggtg gatggcggca ggtactacgt ggtgcgcagg
    12241 cgcgagtcgc tcgatgacgt gatgcgcggc gagtccgtgt tgccggactg agtcgagctt
    12301 ttaaaagcct gaccgcgggt caggccttgc acggccggca acgcgccgcc ctggcgcgtt
    12361 cactgcgggt tgtgcgccgg cgtgagcgca aagccgccgg cgtggaaaat gagcggcgcc
    12421 gcggcgttgt agccgcagtg ctcgacctcg cccaccatga tgaggtggtc gccttcctga
    12481 tactggctgc ggttgcggca ttcgaaccag gcggcgctct cgtcggccag catgagcgtg
    12541 ccgttgggcg ccgttgccag cggcagatcg gcaaagcgct cgggctgctt gccggtggcg
    12601 aagcgccgcg ccagcgcgac atggtcggcc gccagcacgt ggatcacgta gcgctcgcac
    12661 tgcgtcatgg cggggtacga cgaggcctgc agcccgaggc tccacagcac cagcggcggc
    12721 tcgagcgaaa ccgaattgaa ggaactggcg gccatgccga tcggcgagcc gtcggctgcc
    12781 tgggtggtga tgatcgtgac gccggtggcg aaacggccga gcgcggcgcg gaaatgggcg
    12841 gaatcgaaac taggtgtgga tggcatcaca aaagactggg aacatttcaa aaccagtata
    12901 attgaataaa ttatgcaatt caacgaattg acgtcaggag atgccatgga cacgcatagc
    12961 cctcaagccg caacctcgag ctttgctgaa atcccgctga tcgatctggc gcccctcaaa
    13021 gacgggcggc aagcatcgcg cagtgccgtc gcggcggaga tctgcaaggc ctgcgagtcg
    13081 gtcggttttc tttacctggc caaccacggc atcgcggcca gcctcatgga cgacatgttc
    13141 ggcgtcgccc ggcaattctt cgccttgcct ctcgaagaga agatgccgtt gaagctgggc
    13201 gccttttccg ggttccgcgg ctacctgccc gcgggagtcg aaggcggcac catggccggc
    13261 aaccgcaaag aggtgttcca gatcatcaat gagcaggccg acgacggcgc cgggacgctc
    13321 gatccgatgt ggacccagtc gaatctttgg cccgcatcgt ttcccacgct ccggaaagtc
    13381 atgctgcagt acctggccga cgtggagcgg ctttcgttga ccttgctgca attgttcgcg
    13441 atcgggctcg aggttccgga gaccacgttt tcatcgcgct tcaactcccc gatcaacatg
    13501 ctgcgcctgc tccactaccc gccccaggcg ccgacggaca aggccatcgg ctcccagccg
    13561 cacaccgata gcggcgccct gaccatcctc gcgcaggacg acgcgggcgg cctcgaatcg
    13621 gtcgacgacc tcgggcaatg gtcgcaggtc aagccgatca aaaacaccct ggtcgtgaac
    13681 ctcggcgaaa tgctcaagct ctggagcgac ggacggttca gggcgacgcc gcacagggtc
    13741 gtcaatgcgt cgggcaccga tcgcatctcg attccgttct tcgcgagccc ggacatcccc
    13801 acgctgattc agccggtcat ccgcgcgcag acgagaaagg atgagatcca gctggtcggc
    13861 cacctcgagc gcggcacgcc gaagacctcg ggcgagatca tgtccaatag ctggcatcgc
    13921 ctctggggcg gcgcgaagcc cgcgtcgtat tagtattaat tagaccgccg gcttgttgcc
    13981 gcgccattcg cgcacaagcc ccgcctggcg acccgcggcg cttgcttgag ccggggacgc
    14041 cgggcggcga cccgaaccac gggtcgccag cgccgccaga cctcagccgg cgcgccagtt
    14101 cgtgaaacgc tccgtgacct tcgaaccatt gacggattcc cactcggagt tggcgaacgc
    14161 caccatcttg gcgtggacat cggagcccgg cagcgccgcg agcacgtccg gcgtcatgag
    14221 cttgtaggcc tcgggattgc ccgcgccgta gaacaggtag gaactgaagc ccgcctgccg
    14281 cttggcgtcg gcgaggtagt tgatgaactc ccaggccagc ggcgcgtttt tcgcgccctt
    14341 gaccatgccg tagaccacgt ccgacaccag ggcatcgttc cagacgagct ccagcggcac
    14401 gttttgctgc tgcatcatga cggcgcggtt gttccagagc gcaatcatgg agacctcgcc
    14461 gtcgcggatc agctgctcgg cctggctgcc ctgcgaccac cagacgtcga tgtgcggctt
    14521 gatctcgtcg agcttcttga aggcgcgctc gacgtccagg ggatagagct tgtcgtgcgg
    14581 cacgccgtcg gccatcaagg catacgccaa tacccggttc gccatgctcg ccggcaggct
    14641 gcgcgggccg gggaatttct tgacgtccca gaagtcggcc cagctctggg gcccgccgtt
    14701 ggggaacttg tccttgcggt agaccagatt ggtggcgagc gt
//
LOCUS       DASWPO010000049         6747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1885151, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000049 DASWPO010000000
VERSION     DASWPO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885151"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VGC69_02955"
     CDS             <1..689
                     /locus_tag="VGC69_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY0294276.1"
                     /translation="MATRGGYGLSRLLPLIDWKAMADSGKRFVGLSDFTAFNLALLAQ
                     TGTVSYTGPCAAADFGAAKVDELTAALFGEMMRGELEILSFETRDADPVDARGILWGG
                     NLAMVASLIGTPYLPKIKKGILFLEDVAEHPYRVERMLAQLWQAGVLQKQQAIVLGHF
                     SDYRLAPHDAGYDMPEVVKWLRRTVKVPVVTGLPYGHVRTKATLPIGRKVGIATEKGM
                     AHLVIDEHEH"
     gene            complement(702..848)
                     /locus_tag="VGC69_02960"
     CDS             complement(702..848)
                     /locus_tag="VGC69_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294277.1"
                     /translation="MVKANQHEGSSFEEFLHETGDYEEATARALKRVLAEQLQECMAA
                     KIKS"
     gene            complement(841..1113)
                     /locus_tag="VGC69_02965"
     CDS             complement(841..1113)
                     /locus_tag="VGC69_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0294278.1"
                     /translation="MLPAGEERRTIGADIRYVQIKWPIGKPRVDPLRDEIWEVRIHLE
                     NRIARVLFAVAGGKMLLLHGFIKQTQKTPAADIALAHSRYKEWQHG"
     gene            complement(1187..1990)
                     /locus_tag="VGC69_02970"
     CDS             complement(1187..1990)
                     /locus_tag="VGC69_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0294279.1"
                     /translation="MQTVEVVYGPQTAAIWMNRPDVRNALSPTLIAELTQAFQALGKD
                     PALRAIVLAGHGKAFCAGADLNRMKESAQAPEAENRADAQALATLLYTVHTCPKPVIA
                     RIHGPCMAGGLGLISACDIAVATREARFALTETRLGLIPAMISPYVLRALGERAARRW
                     FLTGEAISAAEAWRLGLVHELCETDELDPRVHQLLESFMLASPSALSECKRLIRDVAG
                     KPIDAQIIAETISRLVVQRASPDGREGLASFLEKRKPKWVVERDDDPDA"
     gene            2228..3829
                     /locus_tag="VGC69_02975"
     CDS             2228..3829
                     /locus_tag="VGC69_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEY0294280.1"
                     /translation="MISTANLTIQFGPKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KIIGGDLESSSGNVSLDPGVRLGKLRQDQFAFEDVRVLDVVMMGHTEMWSAMTERDAI
                     YANLEATEDDYMRAADLEAKFAEYDGYTAESRAGELLLGLEIGVDQHNLPMREIAPGW
                     KLRILLAQALFSNPDVLLLDEPTNNLDINTIRWLEDVLNGYQSTMIIISHDRHFLNQV
                     CTHMADLDYREIRIYPGNYDDYMLASTQARERLTANNAKAKERVAELQDFVRRFSANK
                     SKARQATSRLKQIDRIKAEQVEVKPSSRQNPYIRFEQAKVMHRLAVTIEHLSKSYDAP
                     VIKDFSAMVDAGEKVAIIGANGVGKTTLLRMLAGDLAPDSGTIKWSESADPGYMAQDV
                     SDQFQQTDINLFDWMTEHRLPGDDDQSIRSALGRLLFSSDDLPKAPKVLSGGEKNRMT
                     FGRLMLGRHNVMLLDEPTNHLDMESIESLQFALEKYEGTLFFVSHDREFVSGLATRVI
                     EILPGGEVVDYRGAYEDYLASRGVQ"
     gene            complement(3851..5449)
                     /locus_tag="VGC69_02980"
     CDS             complement(3851..5449)
                     /locus_tag="VGC69_02980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0294281.1"
                     /translation="MSASSPEINVDISRRRLLAAAGIGAGALLTSSLASAEGTPAAAD
                     RAAMADPVATPPVAGLHLQFGADASAEMVVSWHTLQPVKQPRVLIGRLDGRLEQTVAA
                     TVSSYVDAKSTQAVYAWHAQVSGLAADTAYLYAALHDGAEPQFGTFRTAPRGRQPLVF
                     TSFGDQGTPTLGKLFAPPAGVTLSGALYVNDNLGSPSAGDTTLGVERLRPLFHLFNGD
                     LCYANLATDRVRAWWDFWNNNTRSARNRPWMPSPGNHENELGNGPIGYQAYQTYFSLP
                     PAAGQTEVTHGLWYAFTAGSVRVIAIANDDVAYQDGGDSYVRGYSGGAQKAWLEKALA
                     AARDDKGIDWLVICMHQVAISTADKFNGADLGIRQEWLPLFDKYGVDLVVCGHEHHYE
                     RSHPIRGRQNNATLTPIPVGTATDVIDTTQGTVHMVIGGGGTSSPSNQLFFNPPCCRV
                     ILGVGDKNPATGRRAPVYETEDAPWSAVRNAAHAYGFAAFDLDPGAHPGDFTTLKVTY
                     YDVVGPDGQLAPFERFTLRRPRRD"
     gene            5535..6731
                     /locus_tag="VGC69_02985"
     CDS             5535..6731
                     /locus_tag="VGC69_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294282.1"
                     /translation="MARDSGSGSTGSITLQILPCVVFTFFCYLSVGLQLAVLPGYVHN
                     VLGFGPVVTGLAVSMQSIATLVTRSHAGGLSDTMGPKRAVLWGQAMGAGSGVLLLMAY
                     AFERSPGFSLGLLLAGRCLLGFCESLVATSGMTWGLGRVGPQHTARVISWNGVSTYSG
                     LALGAPIGVQLAGGFSFGAIGAAVLGVGLISMVIAWGRPDVARQGGVRLPYRSVMARV
                     LPFGLGLGLGTIGLGMLTAFVTLYYASRHWDGAALVLTVFGCSLVLVRLVSGNAIPRF
                     GGFSVALVSLLVEAVGLALIWLAPSPAVVLVGAVFTGMGFSLLFPALGVEVVARMPAG
                     SRGASIGVFSVFFDVALAVAGPLAGYISQGFGFAAIYLAATCTALLAAALVAALRRGW
                     WVGQPA"
BASE COUNT         1063 a         2184 c         2337 g         1163 t
ORIGIN      
        1 tcatggccac gcgcggcggc tatggcttgt cgcgcctgct gccgctgatc gactggaagg
       61 ccatggccga cagcggcaag cgcttcgtgg gcctgagcga tttcacggcc ttcaacctgg
      121 cgctgctggc gcagacgggg acggtcagct ataccggccc ctgcgccgcg gccgatttcg
      181 gcgccgcgaa agtcgacgag ctcaccgcgg cgctgttcgg cgagatgatg cgcggcgagc
      241 tggagatcct cagcttcgaa acccgcgacg ccgacccggt ggatgcccgc ggcatcctgt
      301 ggggcggcaa cctggccatg gtggcctcgc tcatcggcac gccctatctg cccaagatca
      361 agaagggcat tcttttcctg gaagacgtgg ccgagcaccc ctaccgggtg gagcgcatgc
      421 tggcccagct atggcaggcc ggcgtgctgc agaagcagca ggccatcgtg ctggggcact
      481 tcagcgacta ccgcctggcg ccgcacgacg ccggctacga catgcccgaa gtggtcaagt
      541 ggctgcgccg cacggtcaag gtgccggtgg tgacgggcct gccctacggc cacgtgcgca
      601 ccaaggccac gctgcccatc ggccgcaaag tgggcatcgc cacggaaaag ggcatggctc
      661 acctggtgat cgacgagcac gagcattgat tccgcgtcgg ttcaggattt gatcttggct
      721 gccatgcatt cttgaagctg ttcggccagc acgcgtttca aggcacgggc cgtggcctct
      781 tcatagtcgc cggtttcgtg aagaaattct tcgaatgacg atccttcatg ttggtttgct
      841 ttaaccatgt tgccattcct tgtagcggga gtgcgccagc gctatatcgg ccgctggcgt
      901 tttctgcgtc tgcttgatga atccatgcaa caacagcatt tttccgccgg caacggcaaa
      961 aagcacccgg gcgatccggt tttcgagatg gatgcgaacc tcccagatct cgtctcgcag
     1021 agggtccact cgaggtttgc cgatcggcca tttgatctgg acatagcgta tgtcggcgcc
     1081 gattgtgcgg cgctcctcac cggctggaag catgattttc cgaagccgag ggcaagtgga
     1141 acaacagcca tgcttcggca catggccagg ggcattgcag tgccgatcac gcgtccgggt
     1201 cgtcgtcgcg ctccaccacc cacttgggct tgcgcttttc caggaacgag gccaggcctt
     1261 cgcggccatc ggggctggcg cgctgcacca ccagccgcga gatggtttcc gcgatgatct
     1321 gcgcgtcgat gggcttgccc gccacgtcgc ggatcaggcg cttgcattcg gacagcgccg
     1381 acggcgaggc cagcatgaac gattcgagca actggtgcac gcgcgggtcg agctcgtcgg
     1441 tctcgcacaa ttcgtgcacc aggcccagcc gccaggcctc ggcggcggaa atggcctcgc
     1501 ccgtcaggaa ccagcgccgc gcggcgcgct cgcccagggc gcgcagcacg tagggggaaa
     1561 tcatggccgg gatcaggccc agccgggttt cggtgagcgc gaacctggct tcccgggtgg
     1621 ctaccgcgat gtcgcaggcc gagatcagcc ccaggccgcc ggccatgcag gggccgtgga
     1681 tgcgggcgat caccggcttg gggcaggtgt gcacggtata gagcagggtg gccagcgcct
     1741 gcgcgtcggc gcggttttcc gcttccgggg cctgggcgct ttccttcatg cggttcaagt
     1801 cggcgccggc gcagaaggcc ttgccgtggc cggccagcac gatggcgcgg agggcggggt
     1861 ccttgcccag ggcctggaag gcctgggtca actcggcgat cagcgtgggg ctgagcgcgt
     1921 tgcgcacgtc cgggcggttc atccagatgg cggcggtctg cgggccgtag accacttcga
     1981 cggtctgcat cgacgccggc agggcagatt gcgacatggg ggttctcctc ttgcctgtgt
     2041 catgttgtga taatgggcag ggccaacaat accgcagggc gtggggaggc ggaaataggt
     2101 ttgtgcaagc ccggcccgga cccacgccca cgcgcgctct tgttacaatg ccgggttagc
     2161 cactcacggc acccagccat ttcgcgcgcc aaccggcgcg ccgggcctgc ccgcaccgta
     2221 ttccatcgtg atctccaccg ccaacctcac catccagttc ggccccaagc cgctattcga
     2281 aaacgtcagc gtcaagttcg gcgaaggcaa ccgctatggc ctgatcgggg ccaatggctc
     2341 gggcaagtcg acgttcatga aaatcatcgg cggcgacctg gagtcctcca gcggcaatgt
     2401 gtcgctcgac ccgggcgtgc gcctgggcaa gctgcgccag gaccagttcg ccttcgagga
     2461 cgtgcgcgtg ctcgacgtgg tgatgatggg ccacaccgag atgtggtcgg ccatgaccga
     2521 gcgcgacgcc atctacgcca acctcgaggc cacggaagac gactacatgc gcgcggccga
     2581 cctcgaggcc aagttcgccg agtacgacgg ctacaccgcc gaatcgcgcg ccggcgagtt
     2641 gctgctgggc ctggagatcg gcgtggacca gcacaacctg cccatgcgcg agatcgcgcc
     2701 cggctggaag ctgcgcatcc tgctggcgca ggcgctgttc tccaaccccg acgtgctgct
     2761 gctcgacgaa cccaccaaca acctcgacat caacaccatc cgctggctgg aagacgtgct
     2821 caacggctat cagagcacga tgatcatcat cagccacgat cgccacttcc tcaaccaggt
     2881 ctgcacccac atggccgacc tggactaccg cgaaatccgc atctatcccg gcaactacga
     2941 cgactacatg ctggcctcga cccaggcgcg cgagcgcctg acggccaaca acgccaaggc
     3001 caaggagcgc gtggccgagc tgcaggactt cgtgcggcgc ttctcggcca acaagtccaa
     3061 ggcgcgccag gccacctcgc gcttgaagca gatcgaccgc atcaaggccg agcaggtcga
     3121 ggtcaagccc tcctcgcgcc agaaccccta tatccgcttc gagcaggcca aggtcatgca
     3181 ccgcctggcg gtcacgatcg agcatctgtc caagtcctac gacgcgccgg tcatcaagga
     3241 tttctcggcc atggtcgatg cgggcgagaa ggtcgccatc atcggcgcca acggcgtggg
     3301 caagaccacg ctgctgcgca tgctggccgg cgacctggcg cccgactcgg gcacgatcaa
     3361 gtggtccgag agcgccgacc cgggctatat ggcgcaggac gtgtccgacc agttccagca
     3421 gaccgacatc aatctgttcg actggatgac cgagcaccgc ctgcccggcg acgacgacca
     3481 gtccatccgc tcggcgctgg gccggctgct gttctccagc gacgacctgc ccaaggcgcc
     3541 caaggtgctg tcgggcggcg agaagaaccg catgaccttc ggccgcctga tgctgggccg
     3601 gcacaacgtg atgctgctcg acgagcccac caaccacctg gacatggaat cgatcgaatc
     3661 gctgcagttc gcgctggaaa agtacgaagg cacgctgttc ttcgtctcgc acgaccgcga
     3721 gttcgtctcg ggcctggcca cgcgcgtgat cgagatcctg cccggcggcg aggtcgtcga
     3781 ctaccgcggc gcctacgaag actacctggc ctcgcgcggc gtgcaataag cgaaccgcaa
     3841 taagcgaacc tcagtcccgc ctgggccggc gcagggtgaa gcgctcgaag ggcgcaagct
     3901 ggccgtccgg gcccaccacg tcgtaatagg tgaccttgag ggtggtgaaa tcgccggggt
     3961 gcgcgcccgg gtcgagatcg aaggcggcaa agccataggc gtgcgcggca ttgcgcacgg
     4021 ccgaccaggg cgcgtcttcc gtttcataga ccggcgctcg cctgccggtg gcggggttct
     4081 tgtcgcccac gcccaggatg actcgacagc agggcgggtt gaagaagagc tggttggagg
     4141 gcgacgaagt gccgccgccg ccgatcacca tatgcaccgt gccctgggtg gtgtcgatca
     4201 cgtcggtcgc ggtgccgacc gggatcggcg tcagggtggc gttgttctgc cggccgcgga
     4261 tggggtggga gcgctcgtaa tggtgctcgt ggccgcacac caccaggtcc acgccatact
     4321 tgtcgaacag cggcagccat tcctggcgga tgccgagatc ggcgccgttg aacttgtcgg
     4381 cggtggagat ggcgacctgg tgcatgcaga tcaccagcca gtcgatgccc ttgtcgtcgc
     4441 gggcggcggc gagcgctttc tcgagccatg ctttctgggc gccgccggaa tagccgcgca
     4501 cataggaatc gccgccatcc tgataggcga catcgtcatt ggcgatcgcg atcacccgca
     4561 ccgaaccggc ggtaaaggca taccacaggc catgcgtcac ctcggtctgc ccggcggccg
     4621 gcggtagcga gaaataggtc tggtaggcct ggtagccgat cggcccgttg cccagctcgt
     4681 tctcgtggtt gccgggcgaa ggcatccagg ggcggttgcg ggcgctgcgg gtgttgttgt
     4741 tccagaaatc ccaccaggcg cggacgcgat ccgtggcgag attggcgtag cacaggtcgc
     4801 cgttgaacaa gtggaagagg gggcgcagtc gctcgacgcc gagcgtggtg tcgccggcgg
     4861 agggcgagcc gagattgtcg ttgacgtaga gggcgccgct cagcgtgacg ccggcgggcg
     4921 gggcgaacag cttgccgagg gtgggggtgc cctggtcgcc gaagctggtg aacacaaggg
     4981 gctggcgtcc gcgcggcgcg gtgcggaagg tgccgaactg cggctcggcg ccgtcatgca
     5041 gcgcggcgta gagataggcg gtgtcggcgg cgagcccgct gacctgggca tgccaggcat
     5101 agacggcctg cgtcgacttg gcgtcgacat agctcgatac ggtggcggcc accgtctgtt
     5161 cgagcctgcc gtcgagccgg ccgatcagca cgcgcggctg cttcaccggc tgcagcgtat
     5221 gccaggacac caccatctcg gcggacgcgt cggcgccaaa ctgcaggtgc aggccggcta
     5281 ccggcggcgt cgccaccggg tcggccatgg cggcgcggtc ggcggcggcg ggtgttccct
     5341 cggcgctggc cagcgatgag gtcagcagcg caccggcccc gattcccgcg gcggcgagaa
     5401 gcctgcggcg gctgatgtcg acgttgattt caggggagga cgcgctcatc gaggactctc
     5461 aaatagggga taatggcgtg tcacattagc gtggtactgt gacacgacaa ggtcacatag
     5521 gacacggcag catcatggca agggattcag ggtcaggcag cacaggttcc atcaccctcc
     5581 agatcctgcc ctgcgtcgtc ttcacctttt tctgctatct gtcggtcggc ctgcaactgg
     5641 ccgtgctgcc cggctatgtg cacaacgtgc tgggcttcgg cccggtggtc acgggcctgg
     5701 ccgtgagcat gcagtccatc gccaccttgg tgacccgctc gcacgccggc ggcctgtccg
     5761 acaccatggg ccccaagcgc gcggtgctgt ggggccaggc catgggggcg ggcagcggcg
     5821 tgctgctgct gatggcctat gccttcgagc gcagcccggg cttcagcctg gggctgctgc
     5881 tggccggacg ctgcctgctg gggttctgcg aaagcctggt ggccacctcg ggcatgacct
     5941 ggggcctggg ccgcgtcggc ccccagcaca cggcccgcgt catttcctgg aacggcgtgt
     6001 cgacctattc cggcctggcg ctgggcgcgc ccatcggggt gcaactggcc ggcggcttca
     6061 gtttcggcgc catcggcgcg gcggtgctgg gggtgggcct catcagcatg gtcatcgcct
     6121 ggggtcgccc ggacgttgcc cggcaaggcg gcgtgcgcct gccgtaccgc agcgtgatgg
     6181 cgcgggtgct gcctttcggc ctgggcctgg ggctgggcac catcggcctg ggcatgctca
     6241 ccgccttcgt gacgctctac tacgccagcc ggcattggga cggcgccgcg ctggtgctga
     6301 cggtgttcgg ctgctcgctg gtgctggtgc gcctggtgtc gggcaacgcc attccgcgct
     6361 tcggcggctt ttcggtggcg ctggtttcgc tgctggtgga ggccgtgggc ctggccctga
     6421 tctggctggc gccttcgccc gcggtggtgc tggtcggcgc ggtattcacc ggcatggggt
     6481 tctcgctgct gtttccggcc ctgggggtgg aagtggtggc gcggatgccc gccggcagcc
     6541 gcggcgcgtc gatcggggtg ttctcggtct tcttcgacgt ggccctggcg gtggcgggcc
     6601 ccctggcggg ctatatctcg cagggctttg gctttgccgc catctacctg gcggcgactt
     6661 gcaccgcctt gctggcggcc gccctggtgg cggcgctgcg gcgcggctgg tgggtggggc
     6721 agccggcgta gggacgcgct gcatgac
//
LOCUS       DASWPO010000050        22408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3134950, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000050 DASWPO010000000
VERSION     DASWPO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 22408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22408
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3134950"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VGC69_02990"
     CDS             <1..481
                     /locus_tag="VGC69_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY0294283.1"
                     /translation="PARSEWVERWRAAHPGLAGPFEGDHVFCFADGFPILVTNHASLD
                     ELNARLAAQGKPPVSMDRFRPNIVVRGGDWAAFDEDHTVLLRAGAASLALVKPCTRCP
                     VPDVDPLTGERGDEPGLTLAALHTLDLGVVFGMNAIVAEKQPVILKIGDPVDISLDF"
     gene            635..1684
                     /locus_tag="VGC69_02995"
     CDS             635..1684
                     /locus_tag="VGC69_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY0294284.1"
                     /translation="MSFQVTIQSTHHQFPADADQTVLDAALAAGLVLPYSCRTGACST
                     CKGKVVCGEVDAGEYPAQILSPEDLAEGYTLFCQAHAQSDLVIDVAEVRMASDIQIRK
                     LPSRVISMDRAASDVMVIKLQLPASEQFRYYAGQYVDIIMKSGDRRSYSMAGAPHTAT
                     QLELHIRHTPGGKFTDHVFGAGETQMKEREIVRLEGPFGSFFLREDGDKPIVLLASGT
                     GFAPIKAIVEQMIFKKIQRPAVLYWGGRRPQDFYMAELAKSWEAALPDFRYVPVASNA
                     LPEDGWQGRTGFVHQVVMQDIPDMSGYQVYACGAPIVVDSARREFSSQCGLPAEEFYA
                     DSFTSQADLAKAAGA"
     gene            1752..3008
                     /locus_tag="VGC69_03000"
     CDS             1752..3008
                     /locus_tag="VGC69_03000"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY0294285.1"
                     /translation="MKVAVFGSGIIGVASAWWLSQAGHDVVVVDRRSGPARETSLANG
                     AQISVSYAEPWANPQAPLKLLKWMFHDDSPLLFRPQLDWRQWAWGLAFLRECLPSRLA
                     PNIRAMVRLAEYSRSTLQGMRRELGIEYNHLERGILNFYRDPEEFESSQRAAGLMRDF
                     GVERRIVSADEVIAIEPALAPHRANIVGGDYTPEDETGDVHLFTVELARRCEAAGVEF
                     RFDTQVTRLLTAGGQVQAAEVIGADGLYGQVKADAYVAAMGSYTPHLVRPLGVPCNVY
                     PAKGYSATFPVLDPDGAPTVSLTDSSYKVVFSRLGDRIRMAGTAELAGYGRGLNATRC
                     EAMSRLARELFPDALDFENVSYWSGLRPSTPSNVPLIGRTRIPNLFLNTGHGTLGWTM
                     GAGSGRVLADLLSGRTPEPDFPFIGL"
     gene            3014..4384
                     /locus_tag="VGC69_03005"
     CDS             3014..4384
                     /locus_tag="VGC69_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0294286.1"
                     /translation="MKKLQFTRASRAWLFGAALAALIPAAQASTQIQVWHTLTGANKS
                     EFEKLVKQYNKEQGDVEVSLRAFASDDALKKEADTAIRAKQAPNLLQLDDNHSPDVVA
                     EYAAIKPLYDLLAKYPIKDANWYLPATSSFVHDGKGRLLAFPWMAEIPLMFYNIDYYK
                     KAGLNPAQPARTWPDLQNELLKVRDQANVDCPLATSQDVMVHIENLAPVNNQALTAND
                     NGLDNSRAAPAMQFDILYMRHLSTMVSWIRADMYPMHSNDRSADSAFASGQCGVLMSG
                     SGAIGQFLTTSGLKFGVAPLPYYPQATSSPGRPFVSGAALWATEGHPVDQDKATAQFL
                     AWLSKPVIAAEWHQHTGYLPLTEAAFRASDVSYYDRMPGAQAVIASMRTQAPLAGRGF
                     RIHNYDRIEDVFSKEIDDAFAGRTPPVAALNIAAAQAQVLARQGDTSPLAAPAAKTRV
                     AAKKKK"
     gene            complement(4475..5197)
                     /gene="aqpZ"
                     /locus_tag="VGC69_03010"
     CDS             complement(4475..5197)
                     /gene="aqpZ"
                     /locus_tag="VGC69_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEY0294287.1"
                     /translation="MPLSKKCVAEFIGTFWLVLGGCGSAVLAAHVAGADGYPFGIGYA
                     GVALAFGLTVLTGAYAFGGISGGHFNPAVSFGLWAGGRFAGRDLIPYIVAQVLGGILA
                     GFVLYLVATGKSGFTIDSSAAGAFASNGYGARSPGGYSLGAAFLTEAVLTGMFLLVIM
                     GATHKRAAAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAVFVGPEALGQLWLF
                     WVAPIIGGVIGGVIYRLLNSND"
     gene            complement(5349..5975)
                     /gene="ureG"
                     /locus_tag="VGC69_03015"
     CDS             complement(5349..5975)
                     /gene="ureG"
                     /locus_tag="VGC69_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEY0294288.1"
                     /translation="MQQRTKKNPPLRIGIGGPVGSGKTTLLEMLCKAMRDKYDLVVIT
                     NDIYTKEDQRLLTVAGALAPERIMGVETGGCPHTAIREDASINLHAVDVMVEKFPDAD
                     IVFIESGGDNLAATFSPELSDLTLYVIDVAGGEKIPRKGGPGITKSDLLIINKTDLAP
                     LVGASLQIMEEDTRRMRGPRPYVMSDMKSKTGLDKIVGFIEAKGLLAA"
     gene            complement(6018..6656)
                     /locus_tag="VGC69_03020"
     CDS             complement(6018..6656)
                     /locus_tag="VGC69_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEY0294289.1"
                     /translation="MKAQAKRPVPASQVIPRRHTLLEDAYGLAIGIIFVVVGVVFLRS
                     AGLVTGGIAGIALLLSYVLPWTVGDIFTTVNIPFFIFAYFAMGKRFAIKSAIASFGVT
                     IVLGLMPQALTIAAINPLFSALAGGTLCGMGVLALARHGAGVGGTGIVTLWIYRRHGI
                     NAGYGQAAIDCVIMLASASVIVLPLVAWSVLSALALSGMVVAWHRPGRYTGY"
     gene            complement(6656..7333)
                     /locus_tag="VGC69_03025"
     CDS             complement(6656..7333)
                     /locus_tag="VGC69_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HEY0294290.1"
                     /translation="MALRELAALLQLASPALPIGAFSYSQALEAAIEAGLVADAAGAR
                     RWIAAGLDLLAGGEAVSMLKLCQAWRDGDLAAVRRHNDTLLAWRESAELRLETEQMGS
                     SLARLAQDLEWGEPGPRAQLAAVKPLALPTAYAYAATALGLEAAHALTAWLYAWLENQ
                     VSAALKAIPLGQVAGQRILHGLRDAVAQATQRAIDCADEDISTFAPMLGILSARHETQ
                     YSRLFRS"
     gene            complement(7363..8037)
                     /gene="ureE"
                     /locus_tag="VGC69_03030"
     CDS             complement(7363..8037)
                     /gene="ureE"
                     /locus_tag="VGC69_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEY0294291.1"
                     /translation="MLRIEKTLEGNVAPALRKRAASLTLPYEDRCRSRLAVTLDDGRE
                     AALFLPRGTILRHGAMLVATDGTFVQVAAALQPVLRVTSPDPLLLLRAAYHLGNRHTP
                     VQVEADALQLEHDPVLRDMLLRLGVRVAEAQAAFDPESGAYGGGHKHGHDATFEEDHA
                     LAQEAYAHRHGHDHEHSHAHGHEHDHDHVHGPDCDHDHDHDHDHGHDHSHSHGHAHGH
                     KHEHKD"
     gene            complement(8171..9871)
                     /gene="ureC"
                     /locus_tag="VGC69_03035"
     CDS             complement(8171..9871)
                     /gene="ureC"
                     /locus_tag="VGC69_03035"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEY0294292.1"
                     /translation="MARIGRRAYAEMFGPTTGDRVRLADTELLIAVERDYTLYGEEVK
                     FGGGKVIRDGMGQSQRPAAEVADTVITNALILDHWGIVKADIGIKDGRIQRIGKAGNP
                     DIQPGVDIVIGSATEIIAGEGKIVTAGGIDSHVHFICPQLIDEALTAGITTLLGGGTG
                     PATGTNATTITPGPWHIEQMLAAVDGWPINIGLLGKGNTSLPGPLVEQIEAGAIGLKL
                     HEDWGSTPAAIDCALTVADQTDTQVAIHTDTLNEGGFIEATIDAIAGRTIHTYHTEGA
                     GGGHAPDIIKIAGHPNVLPSSTNPTRPYTVNTVDEHLDMLMVCHHLDSGIAEDVAFAE
                     SRIRRETIAAEDILHDLGALSMMSSDSQAMGRIGETIMRTWQTAHKMKVQRGALPGDG
                     LADNTRARRYIAKYTINPAIAHGIAHEVGSVEPGKWADLVLWDPAFFGVKPELILKGG
                     QITMALMGDPNASIPTPQPVHYREMFGARAGALARNSITFVSQAAFDAGVAAKYRLAR
                     RVVPVHGIRDKGKADMIHNNWMPRIEVDPETYQVVADGQVLTCEPAKVLPMAQRYFLF
                     "
     gene            complement(9872..10177)
                     /locus_tag="VGC69_03040"
     CDS             complement(9872..10177)
                     /locus_tag="VGC69_03040"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEY0294293.1"
                     /translation="MIPGELLPLDGEIELNPGRATVSVTVANTGDRPVQVGSHYHFHE
                     VNDALQFDRAAARGFRLNITAGTAVRFEPGQSRTVELVELAGERAVYGFAGKIMGKL"
     gene            complement(10204..10506)
                     /gene="ureA"
                     /locus_tag="VGC69_03045"
     CDS             complement(10204..10506)
                     /gene="ureA"
                     /locus_tag="VGC69_03045"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEY0294294.1"
                     /translation="MKLTPREKDKLLIFTAALLAERRKARGLKLNHPEAVAYISAALM
                     EGARDGRTVADLMDYGATLLTRDDVMEGVPEMIPDVQIEATFPDGTKLVTVHHPIA"
     gene            complement(10555..11454)
                     /locus_tag="VGC69_03050"
     CDS             complement(10555..11454)
                     /locus_tag="VGC69_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEY0294295.1"
                     /translation="MYRPSDAPPQPHSASSPSPSAALAGWQARLHLGFARQGERTVLA
                     ERRHEGPLTVQKPLYPEGGSICHAVIVHPPGGLAAGDQLRITLELRPGAHALLTTPGA
                     TKWYKGTPGRPASQHIDIALQQGARLDWLPQDNIFFDDSHARQQFTLRLAEGATALGW
                     DAALLGRRASGESWRGAGLRALTRIMAPDGRPLWEERQMLHSASPMLDAPQGLDGRPA
                     YGTLWAAGPACVPELAQSLAETLPFDDALRAGATALPGGLLLVRGIAREPEALQRLFR
                     QLWLRLRPLVHGVPGQALRLWAT"
     gene            complement(11728..12903)
                     /locus_tag="VGC69_03055"
     CDS             complement(11728..12903)
                     /locus_tag="VGC69_03055"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEY0294296.1"
                     /translation="MSATYITNARVVLPDTLLENGAVLIEDGVIAAIEPAGSHRAQVV
                     DLQGQWLMPGLVDLHCDAIEKEAEPRSRVLFPLDFALAQVDRRNAIAGITTPYHALSF
                     SNKEWGVRNNATAAELARGIHAFRRQGLVDNRVHLRYEVTDAGALPIIESLIDDEAVD
                     LISVMDHSPGQGQFKTLEAYLQYMMGNHGLDRAQAEEAAQMKLNNGEGALARVERLIA
                     HAQRAGVATASHDDDSVQRIATMRNLGVAISEFPINLDTAKAAVSCGLSTILGAPNVL
                     RGGSQSGSMRAIDGIIAGVTSCLCSDYQPSTLIAAVFTVLEQSGLTMPQAAALVTANP
                     ARAAGLADRGRIQAGLRADLTAVTRLGRLPLVSHVWSAGRLVCATHYPVTREEAVTA"
     gene            complement(12921..13652)
                     /gene="phnL"
                     /locus_tag="VGC69_03060"
     CDS             complement(12921..13652)
                     /gene="phnL"
                     /locus_tag="VGC69_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HEY0294297.1"
                     /translation="MTNASPMIEVRGLRKLFTLHNQGGIRLPVLESVNFEAGAGDCLV
                     LAGPSGAGKSTLLRCLYGNYLATEGSIKVLDNKNPGAPWTDIAQATEQQILALRRDTI
                     AYVSQFLRVIPRVTTLDVVAEPLRQRGADAESARERAAELLARLNVPRRLWSLAPATF
                     SGGEQQRVNIARGLIGGHPILLLDEPTASLDADNRAVVIDLILEARADGRCLIGIFHD
                     EAARDEVATGLLPLKAAAPAMAAAH"
     gene            complement(13655..14431)
                     /gene="phnK"
                     /locus_tag="VGC69_03065"
     CDS             complement(13655..14431)
                     /gene="phnK"
                     /locus_tag="VGC69_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEY0294298.1"
                     /translation="MTATPLLSVRNLAYTWDGIHGCRDVTFDLYPGEVLCVVGESGSG
                     KSTLLSAVSCQVEPESGSVWYDTREDGLVDLKTLSAARRRVLSRTDWGFVRQHARDGL
                     RMQVSAGANIAERLMAVGQRNYAGLRGTAGQWLQKMEIDEGRLDDAPDSFSGGMQQRL
                     QIARNLVTHPRMVFMDEPTASLDVSVQARLLDLLRQLVTDLHLAAIVVTHDLAVARLL
                     AQRTLVMKGGLVVESGLTDQILDDPQHPYTQLLVSSILQS"
     gene            complement(14428..15324)
                     /locus_tag="VGC69_03070"
     CDS             complement(14428..15324)
                     /locus_tag="VGC69_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HEY0294299.1"
                     /translation="MTATAIAAVARDDHYNFGYLDESTKRMVRRALLKAVAIPGYQVP
                     FGSREMPLPYGWGTGGIQVTASIIGPRDVLKVIDQGSDDTTNAINIRRFFGRVTGVQT
                     TESTRQASIIQTRHRVPETPLSEGQVIVFQVPISEPLRWLEPSEVETRTMHSLAEYGA
                     MHVKLYEDIAHYGHIATTYDYPVIVNDRYMMRPSPIPKFDNPKLDNNPALMLFGAGRE
                     KRVYAVPPYTSVKSLDFEDHPFTIEKWSDCCSLCGSRNSFLDEIIVDDRGTRHFVCSD
                     TEYCAERQAGQATDKAAQGGQA"
     gene            complement(15330..16478)
                     /locus_tag="VGC69_03075"
     CDS             complement(15330..16478)
                     /locus_tag="VGC69_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEY0294300.1"
                     /translation="MYVAVKGGERAILNSYKLLDQYRRGDTAVPELTLAQIREQMPLA
                     ISRVMSEGSLYDPHLASLAIKQASGDLIEAIFLLRAYRTTLPRFGYTRPIDTGRMTLQ
                     RRISATFKDLPGGQMLGPTYDYTQRLLDFSLEAEREADDLPPLPQAPERVASRVTDLL
                     QHDALIDPEEPAPGDPPPAVDLTRQPLGFPAGRSARLQNLARGDEGFLLAMGYSTQRG
                     YGNTHPFVAEIRYGTLEVDMHIEELGFDVCLGEIEITECQMVGQFGGAEQECPKLTRG
                     YGLTFGYGERKAMAMSLVDRALRAQELGEAGGNPANDIEFVLYHSDNVEASGFVQHLK
                     LPHYVDFQAELALLRRMHAEHAQAGQTQIHAEVDPDKAYQAATNAEEA"
     gene            complement(16480..17136)
                     /gene="phnH"
                     /locus_tag="VGC69_03080"
     CDS             complement(16480..17136)
                     /gene="phnH"
                     /locus_tag="VGC69_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEY0294301.1"
                     /translation="MNDPHAHAVHSAPGGLAGAHPAAGAAILPGFADAPVDSQTVFRT
                     LLDALAHPGRIYALDVQSGVPAGLSPAMTAALLTLVDIDAPLWLPAGASADMARFLRF
                     HCSSRIMEKPDMATFVAVPAGSAMPALTDCDPGDPAYPDRSATLLIEVESLETGAPLR
                     LAGPGIKDVQMLRPQGLPGDFLAQWKANTLRFPLGVDVILTQGRLVCGLPRTCRIVEA
                     "
     gene            complement(17142..17621)
                     /gene="phnG"
                     /locus_tag="VGC69_03085"
     CDS             complement(17142..17621)
                     /gene="phnG"
                     /locus_tag="VGC69_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEY0294302.1"
                     /translation="MDHLQASRAGSAPEETPRQEWMRVLAQAEPAELDLAFAALGALP
                     AHRWLRKPETGMAMVRARSGGTGAQFNMGELTVTRCALTLDDGTMGVAYVRGRSQRHA
                     EQAALADALLQTGQWHDRLQSRLIEPLARTHAARRDLQARTAAQSKVDFFTLVRGEN"
     gene            17807..18529
                     /gene="phnF"
                     /locus_tag="VGC69_03090"
     CDS             17807..18529
                     /gene="phnF"
                     /locus_tag="VGC69_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEY0294303.1"
                     /translation="MVERGSGIAVWRQIGELLAGDIRNKLYEPGEQLPTEPELAERYS
                     VNRHTIRRAMGELEQEGLVRIEQGRGTFVQEHAIDYAIGRRTRFSENLRRQGLTGHGE
                     LLGKQLLRYAEAAGHLGLARNAELLRVRVRGRSGKRTLSVAEHYLDAERFPDLADKLG
                     ALGSMSKVYAQYGIADYTRKWSRITAVLPDPDLARLLGQPKTRPVLQVEALNVDLEGK
                     PLQYSITRFGGDWVQLVVGDEG"
     gene            18597..19757
                     /locus_tag="VGC69_03095"
     CDS             18597..19757
                     /locus_tag="VGC69_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEY0294304.1"
                     /translation="MHPTLAGITGARVLTSEGLRAASLAFDQGRIAAAGAGALLDAGN
                     LLVLPGIVDLHGDAFERAVMPRPGVTFPYESALHDVDRQLLANGITTEFHGVTLSWEG
                     GLRGAPYAQRMFDVLAGLSPELGAHHYIHLRFETHNVDGVATAQDWIRQGLVRFMAFN
                     DHLPSMVKRLGDDRKLLQYAERAECDLDTFQARIRQAMQSSDKVAGAMRELAATAREA
                     GLRIASHDDPDGVTRRYYNGLDCHVAEFPLTQDAAQVARELGDAIVFGGPNVVRGRSH
                     THAPNATEMIRAGLCDILTSDYYYPAPLAAAMRLVRDGVLPLQQAWNLVSRNPARAAG
                     LDGYGELAAGKAADLVVVDDSNPALPRVCAAIVGGVLRYASREFPIGVRAAA"
     gene            19759..20493
                     /locus_tag="VGC69_03100"
     CDS             19759..20493
                     /locus_tag="VGC69_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HEY0294305.1"
                     /translation="MPLAHRYALYLAPPEPWRSVGDQWLGRSPATGAVLAPAPGADPR
                     QPGWTTEPRRYGLHATLKPPFRLKPGTTVQGLDAAVRALAARAQAFEAPMQCRALRGF
                     LAWCLADDDARTFARMQALGSDAVQSLDVFRAPPTEAELARRRKTPLSPAHVAMLDRW
                     GYPYVFDQFVFHITLSDKLQGQDLEDAGQQVLAISAALLREPMPVRAVSLYVQPEPDA
                     PFLIARHYGFDGTTADCAGAAYLAGD"
     gene            20637..21218
                     /gene="phnN"
                     /locus_tag="VGC69_03105"
     CDS             20637..21218
                     /gene="phnN"
                     /locus_tag="VGC69_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HEY0294306.1"
                     /translation="MSRAAGSGLVYLMGASGCGKDTLLRLARARLSGSDRIAIAQRVI
                     TRPGGADEPSLEVTGPQFAQMSQAGRFAMQWGSHGLLYGIGIEIDERLARGQVVIVNG
                     SRRYLPQALARYPALSAVEIHVDPAVLAQRLAARGRETPEQIAQRLAQAVLPGDPEVP
                     APGVLHKLPNNAAPEAAAQGLLDIAHALLALVQ"
     gene            complement(21268..21816)
                     /locus_tag="VGC69_03110"
     CDS             complement(21268..21816)
                     /locus_tag="VGC69_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0294307.1"
                     /translation="MQTTLPGVTLTRITELFDGYGAAFYGSEAITQTEHALQCARLAE
                     QAGEAPELIAAALLHDLGHLLQASSRIQDMRHQEVAAQALAPLMGPEVIEPIRLHVAA
                     KRYLCAVDAAYYGTLSQASKDSLALQGGPFDDAGAEAFITQPHAEAAVRLRRYDDLAK
                     EVDAATPPLSHYLPLVERLLKA"
     gene            complement(21842..>22408)
                     /gene="phnE"
                     /locus_tag="VGC69_03115"
     CDS             complement(21842..>22408)
                     /gene="phnE"
                     /locus_tag="VGC69_03115"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY0294308.1"
                     /translation="TIQIAIWGTFLAIVCAVPFALLCYSNIVPAWVYQPMRRLMDACR
                     AINEMVFAMLFIAAVGLGPFAGVLALWVHTMGVLAKLFSESVEAIDPRPVEGVRATGA
                     NALEEIIYGVLPQVMPMWVSLSLYRFESNVRSASVLGIVGAGGIGMVLWEIIRSFQYA
                     QTCAVMIIIIIAVVALDTLSARLRKALI"
BASE COUNT         3458 a         7359 c         7989 g         3602 t
ORIGIN      
        1 tccggcccgc tcggagtggg tggagcgctg gcgggcggcg caccccggcc tggccgggcc
       61 gttcgagggc gaccatgtgt tctgctttgc cgacggtttt cccatcctgg tgaccaacca
      121 cgcatcgctg gacgaactca atgcccgcct ggccgcgcag ggcaagccgc cggtgtccat
      181 ggaccggttc cgccccaata ttgtggtgcg gggcggggac tgggcggcct tcgacgaaga
      241 ccacaccgtg ctcctgcggg ccggcgccgc cagcctggcg ctggtcaagc cgtgcacgcg
      301 ctgcccggtg cccgatgtgg acccgctcac gggcgagcgc ggcgacgagc ccggcctgac
      361 cctggccgcc ctgcataccc tggacctcgg cgtggtgttc ggcatgaacg ccatcgtggc
      421 ggaaaaacag ccagttatcc tgaaaatcgg tgatcctgtc gacatttccc tggatttcta
      481 gctggcgcca cgccggccgg ccctgccttg cggggcgata tccgccgctg cccggtccgc
      541 agcctggggc cgtcccctag gggacggccg ccatggggca atagtagaat ggccccttca
      601 tccagccggc atcgtttttt ctgttgaagc gctcatgagt ttccaggtca ccatccagtc
      661 cacccaccat caattcccgg ctgatgccga ccagaccgtg ctcgacgcgg cgcttgccgc
      721 cggactcgtg ctgccctaca gctgccgtac cggcgcttgt tccacctgca agggcaaggt
      781 cgtctgcggc gaggtcgacg cgggcgagta cccggcgcag atcctgtcgc ccgaagacct
      841 ggccgagggc tacacgctgt tctgccaggc ccacgcccag tccgacctgg tgatcgacgt
      901 ggccgaagtg cgcatggcct ccgacatcca gatccgcaag ctgccctcgc gcgtgatctc
      961 gatggaccgc gccgcgtccg acgtgatggt gatcaagctg cagctgccgg cctccgagca
     1021 gttccgctat tacgccggcc agtacgtcga tatcatcatg aagagcggcg accggcgcag
     1081 ctactccatg gccggcgcgc cgcacacggc cacccagctc gagctgcaca tccgccacac
     1141 cccgggcggc aagttcaccg accatgtgtt cggcgccggc gaaacgcaga tgaaagagcg
     1201 cgagatcgtg cgcctggaag gccccttcgg ctccttcttc ctgcgcgagg acggcgacaa
     1261 gcccatcgtg ctgctggcca gcggcacggg cttcgcgccc atcaaggcca tcgtcgaaca
     1321 gatgatcttc aagaaaatcc agcgcccggc cgtgctgtac tggggcggcc gccgcccgca
     1381 ggacttctac atggccgagc tggccaagtc ctgggaagcg gcgctgcccg atttccgcta
     1441 tgtgcccgtg gcctccaacg cgctgcccga agacggctgg cagggccgca cgggcttcgt
     1501 ccaccaggtc gtcatgcagg acatccccga tatgtccggc taccaggtct atgcctgcgg
     1561 cgcgcccatc gtggtcgact cggcccgccg cgaattctcc tcgcagtgcg gcctgcccgc
     1621 cgaggagttc tatgccgatt ccttcacctc ccaggccgac ctggccaagg cagccggcgc
     1681 ctgagccggg cacctgatcc gacgacatcg caggcgttca ccagtcaaga cagacaaagg
     1741 ggcaaagcag catgaaagtc gcggttttcg gtagtggcat catcggcgtg gcaagcgcct
     1801 ggtggctgag ccaggcaggc catgacgtgg tggtcgtgga ccgccgcagc ggcccggcgc
     1861 gcgagaccag cctggccaac ggcgcgcaga tctccgtgtc gtacgccgag ccctgggcca
     1921 atccccaggc gccgctcaag ctgctcaagt ggatgttcca cgacgactcg ccgctgctgt
     1981 tccgcccgca gctggactgg cgccagtggg cctggggcct ggccttcctg cgcgagtgcc
     2041 tgccttcgcg cctggcgccc aatatccgcg ccatggtgcg cctggccgag tacagccgct
     2101 ccaccctgca gggcatgcgc cgcgagctgg gcatcgagta caaccacctc gagcgcggca
     2161 tcctcaactt ctatcgcgat ccggaagagt tcgagagctc gcagcgcgcc gccggcctca
     2221 tgcgcgactt cggcgtcgag cgccgcatcg tcagcgccga cgaggtcatc gccatcgagc
     2281 cggccctggc gccgcatcgc gccaacatcg tgggcggcga ctacacgccc gaggacgaaa
     2341 ccggcgacgt gcatctgttc accgtcgagc tggcccggcg ctgcgaggcc gccggcgtgg
     2401 aattccgctt cgacacgcag gtcacccgcc tgctgacggc cggcggacag gtgcaggcgg
     2461 ccgaggtcat cggcgccgac ggcctctatg gccaggtcaa ggccgacgcc tacgtggcgg
     2521 ccatgggcag ctacacgccg cacctggtgc gcccgctggg cgtgccctgc aatgtctatc
     2581 ccgccaaggg ctattcggcc accttcccgg tgctcgatcc cgacggcgcg cccacggtca
     2641 gcctgaccga cagcagctac aaggtggtgt tctcgcgcct gggcgaccgc atccgcatgg
     2701 cgggcaccgc cgagctggcc ggctacggcc gcgggctcaa cgccacgcgc tgcgaggcca
     2761 tgtccaggct cgcccgcgag cttttccccg acgcgctcga tttcgagaac gtcagctact
     2821 ggtcgggcct gcgcccgtcc acgccctcca acgtgcccct gatcggccgc acccgcatac
     2881 ccaatctgtt tctcaacacg ggacatggta cgctgggctg gacgatgggc gccggttccg
     2941 gacgggttct ggcagacctg cttagcggcc gcacgcccga gcctgatttt ccattcatcg
     3001 gtttgtaggt gtcatgaaga aactgcagtt cacccgcgct tcacgcgcat ggctattcgg
     3061 cgcggcgctg gccgctttga tccccgccgc ccaggcaagc acccagatcc aggtctggca
     3121 tacgctgacc ggcgccaaca agtccgagtt cgagaaactc gtcaagcaat acaacaagga
     3181 gcagggcgac gtcgaggtga gcctgcgcgc tttcgcctcg gacgacgcgc tcaagaagga
     3241 agccgacacg gccatccgcg ccaagcaggc gcccaacctg ctgcagctcg acgacaacca
     3301 ctcgcccgac gtggtggccg agtacgccgc catcaagccg ctgtacgacc tgctggccaa
     3361 ataccccatc aaggacgcca actggtacct gcccgccacg tcgagcttcg tgcacgacgg
     3421 caagggccgc ctgctggcct tcccctggat ggccgaaatc ccgctgatgt tctacaacat
     3481 cgactattac aagaaggccg gcctgaaccc ggcccagccg gcgcgcacct ggcccgacct
     3541 gcagaacgaa ctgctcaagg tccgcgacca ggccaatgtc gattgcccgc tcgccaccag
     3601 ccaggacgtg atggtgcaca tcgagaacct ggccccggtg aacaaccagg cgctgacggc
     3661 caacgacaat ggcctggaca acagccgggc ggcgcccgcc atgcagttcg acatcctcta
     3721 catgcgccat ctgtcgacga tggtgagctg gatccgcgcg gacatgtatc ccatgcacag
     3781 caacgaccgc agcgccgaca gcgctttcgc cagcggccag tgcggggtgc tgatgagcgg
     3841 ctcgggcgcg atcggccagt tcctgaccac cagcggcctg aagttcggcg tggcgcccct
     3901 gccgtactac ccgcaggcca cgtcctcgcc gggccgtccc ttcgtgagcg gcgcggccct
     3961 gtgggctacc gagggccacc cggtcgacca ggacaaggcc acggcgcagt tcctggcctg
     4021 gctgtccaag ccggtgatcg ccgccgagtg gcaccagcac acgggctacc tgccgctgac
     4081 cgaagccgct ttccgcgcct cggatgtgtc ctactacgac cgcatgccgg gcgcccaggc
     4141 cgtgatcgcc tccatgcgca cgcaggcgcc gcttgccggc cgcggtttcc gcatccacaa
     4201 ctacgaccgc atcgaagacg tgttcagcaa ggaaatcgac gatgcctttg ccggcaggac
     4261 cccgccggta gccgcgctga acatcgcggc ggcgcaggcc caggttctgg cacggcaagg
     4321 cgacacgtcg ccgttggccg cgccggccgc caagacccgg gtcgcggcga agaagaaaaa
     4381 gtaatcgtcg cggccatcat gcgaaaacgg ctgccatgtg aactggcagc cgtttttttg
     4441 cctgggggct gcccctgggc aaaagcctcg tgcttcagtc gttgctgttc agcaatcggt
     4501 aaatgacgcc gccgatcacg ccgccgatga tgggggcgac ccagaacagc cacaactggc
     4561 ccagtgcctc gggcccgacg aatacggcaa ccgcggtgga gcgggccgga ttgaccgagg
     4621 tgttggtcac cggaatgctg atcaggtgga tcagcgtgag cgccaggccg atggcgatcg
     4681 gcgcaaagcc cgccgccgcg cgcttgtgcg tggctcccat gatgacgagc aggaacatgc
     4741 ccgtcagcac ggcctcggtc aggaacgcgg cgccgagcga gtagccgccc ggcgaacggg
     4801 cgccatagcc attgctggcg aaggcgccgg ccgcggacga atcgatcgtg aagccggatt
     4861 tgccggtggc caccagatac aggacgaagc cggcgagaat gccgcccagc acctgcgcca
     4921 cgatgtaggg aatcaggtcg cgccccgcga agcggccgcc cgcccagagg ccgaagctga
     4981 ccgccggatt gaaatgcccc cctgaaatgc cgccgaaggc ataagccccg gtcaggacgg
     5041 tcaagccgaa ggccagggcg acgcccgcat agccgatccc gaagggatat ccgtcggcgc
     5101 cggccacatg ggcggccagc acggcgctgc cgcagccccc cagcaccagc cagaacgtac
     5161 caatgaactc cgccacgcac tttttagaca aaggcatccg tattactcct caattgacaa
     5221 tggtaaaggc cggttatcta taaaccgaga gccgattgat tgcaataggt cgaggcggat
     5281 atccccggcg ccgatcgagc tttgcttgcc gctcggatgc gcgccgggcg tgccggcagg
     5341 gcgctccgtc aggcggccag caagcccttt gcttcgatga acccgacgat cttgtccagc
     5401 ccggtcttgg acttcatgtc gctcatcaca tagggccgcg ggccccgcat gcgccgggtg
     5461 tcttcttcca tgatctgcag cgaggcgccc accagggggg ccaggtcggt cttgttgatg
     5521 atcagcaggt cgctcttggt gatgccaggg ccgcccttgc gggggatttt ctcgccgccg
     5581 gccacgtcga tcacgtagag ggtgaggtcg gacagttcgg ggctgaaggt ggcggccagg
     5641 ttgtcgccgc cggactcgat gaagacgatg tcggcatcgg ggaatttctc caccatgacg
     5701 tcgaccgcgt gcaggttgat ggaggcgtcc tcgcggatgg cggtgtgcgg gcagccgccg
     5761 gtctccacgc ccatgatgcg ctcgggcgcg agcgccccgg cgacggtcag caggcgctgg
     5821 tcttccttgg tgtagatgtc gttggtgatg accaccaggt cgtatttgtc gcgcatggcc
     5881 ttgcacagca tctcgagcag ggtggtcttg cccgagccga ccgggccgcc gatgccgatg
     5941 cgcaggggtg ggttcttctt ggtgcgttgt tgcatgtggg tctctcgtgg tgggtagggg
     6001 ccggcgcgtg gccgcgttca atagccggtg tagcgcccgg gcctgtgcca ggccacgacc
     6061 atgccactca gcgccagggc gctcaggacg gaccaggcca ccagcggcag gacgatgacg
     6121 ctggccgagg ccagcatgat gacgcaatcg atggcggcct ggccatagcc ggcgttgatg
     6181 ccgtgccggc ggtagatcca cagcgtgacg atgccggtgc cgcccacgcc cgcgccgtgg
     6241 cgcgccaggg ccagcacgcc catgccgcac agggtgccgc cggccagcgc cgagaacagc
     6301 ggattgatgg ccgcgatggt cagcgcctgg ggcatcaggc cgagcacgat ggtgacgccg
     6361 aagctggcga tggccgactt gatggcgaag cgcttgccca tggcgaagta ggcaaagatg
     6421 aagaagggaa tgttgaccgt ggtgaagatg tcgcccaccg tccagggcag cacataggac
     6481 agcagcagcg cgatgccggc gatgccgccc gtcaccagcc ccgccgaccg caggaacacc
     6541 acgccgacga cgacgaagat gatgccgatg gccaggccgt aggcgtcttc caggagggtg
     6601 tgccgccgcg ggatgacctg gctggcggga accgggcgtt tggcttgggc cttcatcagg
     6661 aacggaacag gcgcgaatat tgggtttcgt gccgcgcgga gaggatgccc agcatggggg
     6721 cgaaggtgct gatgtcttcg tccgcgcaat cgatggcgcg ttgcgtggcc tgggccacgg
     6781 catcgcgcag gccgtgcagg atgcgctgcc cggccacctg gcccagcgga atggccttga
     6841 gcgcggccga gacctggttt tccagccagg catagagcca ggcggtcagg gcgtgcgcgg
     6901 cctccagccc cagggcggtg gcggcgtagg cataggcggt cggcagcgcc agcggcttga
     6961 cggcggcgag ctgggcgcgc gggccgggct cgccccattc gaggtcttgc gccaggcgcg
     7021 cgagcgaact gcccatctgc tcggtttcca ggcgcagctc ggcggattcg cgccaggcca
     7081 gcagggtgtc gttgtgccgg cgcacggcgg cgaggtcgcc gtcgcgccag gcctggcaca
     7141 gcttgagcat cgatacggcc tcgccgccgg ccagcaggtc caggcccgcg gcgatccagc
     7201 gccgggcgcc ggcggcgtcg gcaaccaggc cggcctcgat ggcggcctcc agcgcctgcg
     7261 aatagctgaa ggcgccgatg ggcagcgcgg gcgaagccag ctgcagcagg gcggccagct
     7321 cacgcagggc caggccgccg gcagggtccg tggaagaggc catcaatcct tgtgctcatg
     7381 cttgtggccg tgggcatggc cgtggctgtg cgagtgatca tggccgtgat catggtcatg
     7441 atcgtgatcg tgatcgcagt ccgggccatg gacgtgatcg tgatcatgct cgtggccatg
     7501 cgcatggctg tgctcatgat cgtggccatg ccggtgcgca taggcttcct gcgccagcgc
     7561 atggtcttcc tcgaaggtcg cgtcgtgccc atgcttgtgg ccgccgccat aggcgccgga
     7621 ctcggggtcg aaagcggctt gcgcctcggc tacccgcact cccaggcgca gcagcatgtc
     7681 gcgcagcacc ggatcgtgct ccagctgcaa ggcatcggcc tcgacctgca cgggcgtgtg
     7741 gcgattgccc aaatggtagg cggcgcgcag cagcaaaagc ggatcggggc tggtgacgcg
     7801 cagcacgggt tgcaaggccg cagccacttg aacgaacgtc ccgtcggtcg ccaccagcat
     7861 ggcgccgtgc cgcaggatgg tgccgcgcgg caggaacagc gccgcttcgc ggccgtcatc
     7921 cagcgtcacc gccaggcggc tgcggcaacg atcctcataa ggcaaagtca acgaagcggc
     7981 acgcttgcgc aaagccggcg cgacattgcc ttccaatgtt ttttcaatac gtagcatgat
     8041 gaccactatg agccgaggac cccggagcaa tccgaagcgc aggcgctacc agcgccgagc
     8101 atcggaattg cgcagggcag cctcggcggc taaagaactc gaataaacga acgaaacgaa
     8161 caaacaaacg ctaaaacaaa aaataccgct gcgccatggg cagcaccttg gccggctcgc
     8221 aagtcagcac ctgcccatcg gccaccacct ggtaggtctc cggatcgacc tcgatcctgg
     8281 gcatccagtt attgtggatc atatcggcct tgcccttgtc gcggatgccg tgcaccggca
     8341 ccacgcgccg ggccaggcgg tacttggccg ccacgccggc atcgaaagcc gcctgcgaca
     8401 cgaacgtgat ggaattgcgc gccaaagccc cggcgcgcgc gccgaacatc tcgcggtaat
     8461 gcaccggctg cggcgtggga atggacgcgt tggggtcgcc catcagcgcc atggtgatct
     8521 gtccgccctt gagtatcagc tcgggcttca cgccgaagaa ggccggatcc cacagcacca
     8581 ggtcggccca cttgccgggc tccaccgagc ccacctcgtg ggcgatgccg tgggcgatgg
     8641 cggggttgat ggtgtacttg gcgatgtagc ggcgcgcgcg ggtgttgtcg gccaggccgt
     8701 cgccgggcag ggcgccgcgc tgcaccttca tcttgtgggc cgtctgccag gtgcgcatga
     8761 tggtctcgcc gatgcggccc atggcctgcg aatccgacga catcatcgac agcgcgccca
     8821 ggtcgtgcag gatgtcctcg gccgcgatgg tctcgcggcg gatgcgcgat tcggcaaagg
     8881 ccacgtcctc ggcgatgccc gaatccaggt ggtggcacac catcagcatg tccaggtgct
     8941 cgtcgaccgt gttcacggtg tagggccgcg tggggttggt cgaggagggc agcacattgg
     9001 ggtggcccgc gatcttgatg atgtcgggcg catggccgcc gcccgcgcct tcggtatggt
     9061 aggtgtggat ggtgcggccg gcgatggcgt cgatggtggc ctcgatgaag ccgccctcgt
     9121 tgagcgtgtc ggtgtggatg gccacttgcg tgtcggtctg gtcggccacg gtgagcgcgc
     9181 agtcgatggc ggccggcgtc gagccccagt cttcgtgcag tttcaggccg atggcgccgg
     9241 cctcgatctg ctcgacgagc gggccgggca ggctggtgtt gcccttgccc aggaggccga
     9301 tgttgatggg ccagccgtcc accgcggcca gcatctgttc gatgtgccag gggccgggcg
     9361 tgatggtggt ggcgttggtg ccggtggccg ggcccgtgcc gccgcccagc agggtggtga
     9421 tgccggcggt gagcgcttcg tcgatgagct gcgggcagat gaagtgcacg tgcgagtcga
     9481 tgccgcccgc ggtgacgatc ttgccttcgc cggcgatgat ctcggtggcc gagccgatga
     9541 cgatgtccac gcccggctgg atgtcggggt tgccggcctt gccgatgcgc tggatgcggc
     9601 cgtccttgat gccgatgtcg gccttgacga tgccccagtg gtcgaggatg agcgcgttgg
     9661 tgatgacggt gtcggccact tccgcggccg ggcgctgcga ctggcccatg ccgtcgcgga
     9721 tcaccttgcc gccgccgaac ttcacttcct cgccgtagag ggtgtagtcg cgctcgacgg
     9781 cgatcagcaa ttccgtgtcg gccaggcgca cccggtcgcc ggtggtgggg ccgaacattt
     9841 ccgcgtaggc gcggcgaccg atcctggcca tttagagttt ccccatgatt ttgccggcaa
     9901 agccgtagac ggcgcgttcg ccggcgagtt cgacgagctc caccgtgcgc gattggccgg
     9961 gttcgaagcg cacggcggtg ccggcggtga tgttcaggcg aaagccgcgc gcggcggcgc
    10021 ggtcgaactg cagcgcgtcg ttgacttcgt ggaagtgata gtgcgaaccc acctgcaccg
    10081 ggcggtcgcc ggtattggcc accgtgacgc tgacggtggc gcggccgggg ttgagttcga
    10141 tctcgccgtc tagcggcagc agttctccgg gaatcatgat ggacctcgct gatgggcttg
    10201 gatttaggcg atgggatggt gcacggtgac cagcttggtg ccgtcgggga aggtggcttc
    10261 gatctgcacg tcgggaatca tttcaggcac gccttccatg acgtcgtcgc gcgtgagcag
    10321 ggtggcgccg tagtccatga ggtcggccac ggtgcggccg tcgcgggcgc cttccatcag
    10381 cgcggcgctg atataggcca cggcctccgg gtggttgagc ttcaggccgc gcgccttgcg
    10441 gcgttcggcc agcagcgcgg cggtgaagat cagcagtttg tctttttctc gcggggtcag
    10501 tttcatgaag ggtcagccgt atgagcgatc gcgggtcggg aaaggggtga ggggtcaggt
    10561 ggcccagagg cgcaaggcct ggccgggcac gccgtgcacg agcggccgca ggcgcagcca
    10621 tagctgcctg aagagccgct gcagcgcttc gggttcgcgg gcgatgccgc gcaccagcag
    10681 caggccgccc ggcagggcgg tggcgccggc gcgcagcgcg tcgtcgaaag gcagggtctc
    10741 ggccagcgat tgcgccagct cgggcacgca ggccgggccg gcggcccaga gcgtgccgta
    10801 ggccggccgg ccgtccaggc cttgcggggc gtcgagcatg gggctggcgc tgtgcagcat
    10861 ctggcgttct tcccagagcg ggcggccgtc gggggccatg atgcgcgtca gcgcgcgcag
    10921 gccggcgccg cgccaggact cgccgctggc gcggcgcccc agcagggcgg catcccagcc
    10981 cagcgcggtg gcgccttcgg ccaggcgcag ggtgaattgc tgccgcgcat ggctgtcgtc
    11041 gaagaagata ttgtcctgcg gcagccagtc gagccgggcg ccttgctgca gggcgatgtc
    11101 gatgtgctgg ctggcgggcc gcccgggcgt gcccttgtac cacttggtgg cgccgggggt
    11161 ggtgagcagc gcatgggcgc cgggccgcag ctccagcgtg atgcgcaatt ggtcgccggc
    11221 cgccagcccg ccgggcgggt gcacgatgac ggcatggcag atgctcccgc cttccgggta
    11281 gagcggcttt tgcacggtga gcgggccttc gtggcggcgc tcggccagca cggtgcgttc
    11341 gccctgccgg gcaaagccca ggtgcagccg cgcctgccag cccgcgagcg ccgcgctggg
    11401 cgacggactg ctggcggagt gcggctgcgg cggtgcgtcg gaggggcggt acatgggggt
    11461 gggcgtaaaa aaccggggaa ggatccccgg tcgagtgtgg catatttgcc ggcccgcgcc
    11521 aatccgggat gctgcttggg ttctttagtc gcgtcgacgt attgcggggg gagcccagcg
    11581 gggcctgcgg ggcttcactg tgccgggctc gggcccaaga tcacgggccc tcggcccctg
    11641 cggggctgcc ccgtcccaga ggcgccccgc aggccccgct gggctccccc cgcaacacgt
    11701 cgacgcttgc gatagctgct caagttctca agccgtcacg gcctcttccc gcgtcaccgg
    11761 atagtgcgtg gcgcacacca ggcggccggc cgaccacacg tggctgacca gcggcaggcg
    11821 tcccagcctg gtgacggcgg tcaggtcggc gcgcaggccc gcctggatgc ggccgcggtc
    11881 ggccaggccg gcggcgcggg ccgggttggc ggtgaccagg gcggcggcct gcggcatggt
    11941 caggccggac tgttccagca cggtgaagac cgcggcgatc agggtggacg gctggtagtc
    12001 cgagcacagg cagctcgtca cgccggcgat gatgccgtcg atggcgcgca tcgagccgct
    12061 ctggctgccg ccgcgcagca cgttgggcgc gcccaggatg gtggacaggc cgcaggacac
    12121 ggcggccttg gccgtatcca ggttgatggg gaactcgctg atggccacgc ccaggttgcg
    12181 catggtggcg atgcgctgca ccgaatcgtc gtcatggctg gcggtggcca cgcccgcgcg
    12241 ctgggcgtgg gcgatcaggc gctcgacgcg ggccagcgcg ccttcgccgt tgttcagctt
    12301 catctgcgcg gcttcttcgg cctgggcgcg gtccaggccg tggttgccca tcatgtattg
    12361 cagataggct tccagggtct tgaactggcc ctggccgggc gagtggtcca tgaccgagat
    12421 caggtccacc gcctcgtcgt cgatgagcga ttcgatgatg ggcagggcgc cggcgtcggt
    12481 gacctcgtag cgcaggtgca cgcggttgtc caccaggccc tgcctgcgga aggcgtggat
    12541 gccgcgcgcc agctcggcgg cggttgcgtt gttgcgcacg ccccattcct tgttggagaa
    12601 cgacagggca tggtagggcg tggtgatgcc ggcgatggcg ttgcggcggt cgacctgggc
    12661 cagggcgaag tccagcggaa acagcacgcg cgaacgcggc tcggcttctt tctcgatggc
    12721 gtcgcagtgc aggtcgacca ggccgggcat gagccattgg ccttgcaggt cgacgacctg
    12781 ggcacggtgg ctgccggccg gctcgatggc ggcgatgacg ccgtcttcga tcagcacggc
    12841 gccgttttcc agcagggtgt cgggcaggac gacgcgggcg ttggtgatgt aggtggcgga
    12901 catgggcgat ggattccgga tcagtgcgcg gcggccatgg cgggcgcggc cgccttcagg
    12961 ggcaggaggc cggtggcgac ttcatcgcgc gcggcctcgt cgtggaagat gccgatcagg
    13021 cagcggccgt cggcgcgggc ttcgaggatc aggtcgatca ccacggcgcg gttgtcggcg
    13081 tccagcgagg cggtgggctc gtccagcagc aggatggggt ggccgccgat caggccgcgg
    13141 gcgatgttca cgcgctgctg ctcgccgccc gagaaggtgg cgggcgccag gctccagagg
    13201 cgcctgggca cgttcaggcg ggcgagcagt tcggcggcgc gctcgcgggc cgattcggca
    13261 tcggcgccgc gctggcgcaa gggctcggcc accacgtcca gcgtggtgac gcgcggaatc
    13321 acgcgcagga actggctcac gtaggcgatg gtgtcgcggc gcagggccag gatctgctgt
    13381 tccgtggcct gggcgatgtc cgtccacggc gcgcccgggt ttttgttgtc gagcaccttg
    13441 atgctgcctt ccgtggccag gtagttgccg tagaggcagc gcaagagcgt gctcttgccc
    13501 gcgcccgagg ggccggccag caccaggcag tcgccggcgc cggcttcgaa gttcaccgat
    13561 tccagcaccg gcaggcggat gccgccctgg ttgtgcaggg tgaacagttt gcgcagaccg
    13621 cgcacttcta tcatgggaga ggcgttcgtc atgatcagct ctgcaaaatg gacgagacga
    13681 gcagctgcgt ataggggtgc tgcgggtcgt ccaggatttg gtcggtgagg ccgctttcga
    13741 ccaccaggcc gcccttcatg accagcgtgc gctgcgccag gaggcgcgcc acggccaggt
    13801 cgtgggtgac gacgatggcg gcaaggtgca ggtcggtgac gagctggcgc agcaggtcca
    13861 ggaggcgcgc ctgcaccgac acgtccagcg aggcggtggg ttcgtccatg aacaccatgc
    13921 gcggatgcgt gaccaggttg cgggcgatct gcaggcgctg ctgcatgccg cccgagaacg
    13981 agtcgggcgc atcgtccagg cggccttcgt cgatttccat tttctgcagc cattggccgg
    14041 cggtgccgcg caggccggcg tagttgcgct ggcccacggc catcaggcgt tcggcgatgt
    14101 tggcgccggc gctcacctgc atgcgcaggc cgtcgcgggc gtgctggcgc acgaagcccc
    14161 agtcggtgcg cgacagcacg cggcggcgcg cggccgacag cgttttcagg tcgaccaggc
    14221 cgtcctcgcg ggtgtcgtac cagacgctgc ccgattcggg ctccacctgg cacgagacgg
    14281 ccgatagcag cgtggacttg cccgagccgg attcgcccac cacgcacagc acttcgccgg
    14341 ggtagaggtc gaaggtcacg tcgcggcagc cgtggatgcc gtcccaggtg taggcgaggt
    14401 tgcgcacgga gagcagcggc gtggcggtca tgcctggcct ccctgggcag ccttgtccgt
    14461 ggcctgtccg gcctggcgtt cggcgcagta ttcggtgtcc gagcatacga agtggcgcgt
    14521 gccccggtcg tcgacgatga tctcgtcgag gaagctgttg cgcgagccgc acaggctgca
    14581 gcagtccgac cacttctcga tggtgaaggg gtggtcttcg aaatccaggc tcttcacgga
    14641 ggtatagggc ggcacggcgt acacgcgctt ttcccggccg gcgccgaaca gcatcagcgc
    14701 ggggttgttg tcgagcttgg gattgtcgaa cttggggatg ggcgagggcc gcatcatgta
    14761 gcggtcgttg acgatcaccg ggtagtcgta ggtggtggcg atgtggccgt agtgcgcgat
    14821 gtcttcgtac agcttgacgt gcatggcgcc gtattcggcc agcgagtgca tggtgcgggt
    14881 ctcgacctcg ctgggttcga gccagcgcaa gggttccgag atcggcacct ggaacacgat
    14941 cacctggccc tcggacagcg gggtctcggg gacgcggtgg cgcgtctgga tgatgctggc
    15001 ctgccgggtg gattccgtgg tttgcacgcc cgtcacgcgg ccgaagaagc ggcggatgtt
    15061 gatggcgttg gtggtgtcgt ccgaaccctg gtcgatgacc ttgagcacgt cccgggggcc
    15121 gatgatggac gcggtgacct ggatgccgcc cgtgccccag ccgtagggca ggggcatttc
    15181 gcggctgccg aagggcacct ggtagccggg gatggccacg gccttgagca gggcgcggcg
    15241 caccatgcgc ttggtggatt cgtccaggta gccgaagttg tagtggtcgt cgcgggcgac
    15301 ggcggcaatg gcggttgcgg tcatgatgtt caagcctcct cggcgttggt ggcggcctgg
    15361 taggctttgt cggggtcgac ttcggcgtgg atttgggttt ggcctgcctg ggcgtgctcg
    15421 gcgtgcatgc ggcgcagcag cgcgagctcg gcctggaagt ccacgtagtg cggcagcttg
    15481 aggtgctgca cgaagccgga ggcctcgacg ttgtcgctgt ggtagagcac gaactcgatg
    15541 tcgttggccg ggttgccgcc ggcctcgccc agttcctggg cgcgcagcgc gcggtcgacc
    15601 agcgacatgg ccatggcctt gcgctcgccg tagccgaagg tcaggccgta gccgcgggtg
    15661 agcttggggc attcctgctc ggcgccgccg aactggccga ccatctggca ctcggtgatt
    15721 tcgatctcgc ccaggcacac gtcgaagccc agctcctcga tgtgcatgtc gacttccagc
    15781 gtgccgtagc ggatctcggc cacgaagggg tgcgtgttgc cgtagccgcg ctgcgtggag
    15841 tagcccatgg ccagcaggaa gccctcgtca ccgcgcgcca ggttctgcag gcgcgcgctg
    15901 cggccggccg gaaagcccag cggctggcgc gtcaggtcga cggccggcgg cgggtcgccc
    15961 ggcgcgggtt cctcggggtc gatgagggcg tcatgctgca gcaggtcggt cacgcgcgag
    16021 gccacgcgct cgggcgcctg cggcaggggc ggcaggtcgt cggcctcgcg ctcggcttcc
    16081 aggctgaagt ccagcaggcg ctgcgtgtag tcgtaggtcg ggcccagcat ctggccgccg
    16141 ggcaggtcct tgaaggtggc cgaaatgcgg cgctgcaggg tcatgcggcc ggtgtcgatg
    16201 gggcgcgtat agccgaagcg cggcagcgtg gtgcggtagg cgcgcagcag gaagatggct
    16261 tcgatcaggt cgccgctggc ctgcttgatg gccagcgacg ccaggtgcgg gtcgtacagc
    16321 gagccttcgc tcatgacgcg cgagatggcc agcggcatct gctcgcggat ctgcgccagc
    16381 gtgagctcgg gcacggcggt gtcgccgcgg cggtactggt cgagcagctt gtaggaattg
    16441 aggatggcgc gttcgccacc tttgacggca acgtacatgt catgcctcca cgatgcggca
    16501 ggtgcggggc aggccgcaca ccaggcggcc ttgggtcagg atgacgtcga cacccagcgg
    16561 aaagcgcagg gtgttggctt tccattgcgc gaggaaatca ccgggcaggc cttgcgggcg
    16621 cagcatctgc acgtccttga tgccggggcc ggccaggcgc agcggcgcgc cggtttccag
    16681 cgattcgact tcgatgagca gcgtggcgga gcggtccggg taggccgggt cgcccggatc
    16741 gcaatcggtg agcgcgggca tggcgctgcc ggcaggcacg gccacgaagg tggccatatc
    16801 gggcttttcc atgatgcggc tgctgcaatg aaagcgcagg aagcgcgcca tgtcggcgct
    16861 ggcgccggcc ggcagccaca gcggggcgtc gatatcgacc agggtcagca gcgcggcggt
    16921 catggccggc gacaggccgg ccggcacgcc gctctgcacg tccagcgcat agatgcggcc
    16981 gggatgcgcc agggcatcga gcagcgtgcg gaatacggtt tgcgaatcga cgggcgcgtc
    17041 ggcgaagccg ggcaggatgg cggcgccggc ggcgggatgg gcgccggcaa gcccgcccgg
    17101 agcactgtga acggcgtgag cgtgggggtc gttcatgatt ttcagttttc tcctcggacc
    17161 agcgtgaaga agtccacctt ggattgcgcg gcggtgcgcg cctgcagatc gcggcgcgcg
    17221 gcatgggtac gggccagggg ctcgatcagc cggctttgca ggcggtcgtg ccattgcccg
    17281 gtttgcagca gggcgtcggc cagggcggcc tgttcggcat ggcgctgcga gcggccgcgc
    17341 acataggcca cgcccatggt gccgtcgtcc agggtcaggg cgcagcgcgt gacggtgagt
    17401 tcgcccatat tgaactgggc gccggtgccg ccgctgcggg cccgcaccat ggccatgccg
    17461 gtctcgggct tgcgcagcca ccgatgggcg ggcagcgcgc ccagggcggc gaaggcgagg
    17521 tcgagttcgg cgggctcggc ctgggccagg acccgcatcc attcctggcg aggggtttct
    17581 tcgggcgcgg agccggcgcg ggaggcttgg agatggtcca tatttcctct aaacgtctag
    17641 acgattgtga tagtatgctc cctgtggacc ccgatattca agccccgcgg cgtgaactta
    17701 tgatgacaag ttgtcatgcc cgccgcgtat aacggcggca catggccgaa aaccgtggca
    17761 ggcacggcat ggcaaagaca catacatcaa tcgggaagcg tcgaacatgg ttgaacgggg
    17821 ttcaggtatc gcggtgtggc gccagatagg cgaactgctg gcgggtgata tacgcaacaa
    17881 gctctacgag ccgggcgagc agctgcccac cgagcccgag ctggccgaac gctattcggt
    17941 caaccgccac accatccgcc gcgccatggg cgagctcgag caggaggggc tggtgcgcat
    18001 cgagcagggc cgcggcacct tcgtgcagga acacgccatc gactacgcca tcggcaggcg
    18061 cacgcgcttt tccgagaacc tgcgccgcca ggggctgacc gggcacggcg aactgctggg
    18121 caagcaattg ctgcgctacg ccgaagcggc cggccacctg gggctggcgc gcaacgccga
    18181 actgctgcgc gtgcgcgtgc ggggacgctc cggcaagcgc acgctcagcg tggccgagca
    18241 ctatctcgac gcggaacgct ttcccgatct ggccgacaag ctcggcgccc tgggctcgat
    18301 gtccaaggtc tacgcgcaat acggcatcgc cgactacacc cggaaatggt cgcgcatcac
    18361 ggccgtgctg cccgaccccg acctggcccg cctgctgggc cagcccaaga cacgcccggt
    18421 gctgcaggtc gaggcgctga acgtcgacct ggaaggcaag ccgttgcaat acagcattac
    18481 gcgcttcggc ggcgactggg tgcaactggt cgtcggcgac gagggttgat tcatctatac
    18541 atctggccgt catttgcctc ttctagtatc cgaacttcac agaaaggagc ttagtcatgc
    18601 atcccaccct ggccggcatc accggcgccc gcgtactcac cagcgaaggc ctgcgggccg
    18661 cctcgctggc attcgaccag ggccgcatcg ccgcggccgg cgcgggcgcg ctgctcgatg
    18721 caggcaatct gctggtgctg cccggcatcg tcgatctgca tggcgatgcc ttcgaacgcg
    18781 ccgtgatgcc gcgtcccggg gttaccttcc cctatgaaag cgcgctgcac gatgtcgacc
    18841 gccaactgct ggccaacggc atcaccaccg aattccacgg ggtgaccctg tcctgggagg
    18901 gcggcctgcg cggcgcgccc tacgcgcagc gcatgttcga cgtgctggcc ggcctgagcc
    18961 ccgaactggg cgcgcaccac tacatccacc tgcgcttcga gacccacaac gtcgacggcg
    19021 tggccacggc gcaggactgg atccgccagg gcctggtgcg cttcatggcc ttcaacgacc
    19081 acctgcccag catggtcaag cgcctgggcg acgatcgcaa gctgctgcaa tacgccgagc
    19141 gcgccgagtg cgatctcgac accttccagg cccgcatccg ccaggccatg cagtcgtcgg
    19201 acaaagtggc cggcgccatg cgcgaattgg ccgccacggc gcgcgaggcc ggcctgcgca
    19261 tcgcgtcgca cgacgatccc gacggcgtca cccgccgcta ctacaacggg ctggattgcc
    19321 acgtggccga gttcccgctc acgcaggatg ccgcgcaggt ggcccgcgag ctgggcgacg
    19381 ccatcgtgtt cggcgggccg aacgtggtgc gcggccgcag ccacacgcac gcgcccaacg
    19441 ccaccgagat gatccgcgcg ggcctgtgcg acatcctgac gtccgactat tactatcccg
    19501 cgccgctggc ggcggccatg cgcctggtgc gcgacggcgt gctgcccttg cagcaggcct
    19561 ggaacctcgt gtcgcgcaac ccggcccggg ccgccggcct ggacggatat ggcgagctgg
    19621 cggcgggcaa ggcggccgac ctcgtcgtgg tcgacgatag caacccggcc ctgccgcgcg
    19681 tctgcgcggc gatcgtgggc ggcgtgctgc gctatgccag ccgcgagttc ccgatcggcg
    19741 tgcgcgcggc agcctgacat gccgctcgcc catcgctatg ccctgtatct ggcgccgccc
    19801 gagccctggc gctccgtggg cgaccagtgg ctgggccgca gcccggcaac cggcgccgtg
    19861 ctggcgccgg ctcctggcgc cgaccctcgg caacccggct ggacgaccga gccgcgccgc
    19921 tacggcctgc acgccacgct caagccgccg ttccgcctga aacccggcac cacggtccag
    19981 ggcctggacg ccgccgtgcg cgccctggcc gcgcgcgcgc aggccttcga ggcgcccatg
    20041 cagtgccgcg cgctgcgcgg ctttctggcc tggtgcctgg ccgacgatga cgcgcggact
    20101 ttcgcgcgca tgcaggcgct gggttccgac gccgtgcagt cgctggatgt ttttcgcgcg
    20161 ccgcccaccg aggccgagct ggcgcgccgc cgcaagacgc cgctgagccc ggcgcacgtg
    20221 gccatgctgg atcgctgggg ctatccctat gtgttcgacc agttcgtgtt ccacatcacc
    20281 ctgtcggaca aattgcaggg gcaggatctc gaagacgccg ggcaacaggt gctggccatc
    20341 agcgccgcgc tgctgcgcga gcccatgccg gtgcgggcgg tgagcctgta cgtgcagccc
    20401 gagcccgacg cgcctttcct catcgcgcgg cattacggct tcgacggcac gacggccgac
    20461 tgcgccggcg cggcctattt ggcgggggac tagcatgagc gcgccgagcc gcctcaagcg
    20521 ctcattcccc ctcggggggt cggcgcgcag cgccaagggg gcaacatgag cgcgccgagc
    20581 cgcctcaagc gcacattccc cctcgggggg acggcgcgca gcgccaaggg ggcaacatga
    20641 gccgcgccgc cggttccggc cttgtctatc tgatgggcgc ttcgggctgc ggcaaggaca
    20701 ccctgctgcg cctggcgcgg gcacggctgt ccggttcgga ccgcatcgcg atcgcgcaac
    20761 gggtcatcac ccggcccggc ggcgccgacg agcccagcct ggaagtgacc gggccgcaat
    20821 tcgcgcagat gagccaggcc gggcgctttg ccatgcaatg gggcagccac ggcctgcttt
    20881 acggcatcgg catcgagatc gacgagcgcc tggcccgcgg acaggtggtg atcgtcaacg
    20941 gttcgcgccg ctacctgccg caggcgctgg cacgctatcc ggctttgtcg gcagtggaaa
    21001 tccacgtcga tccggcggtg ctggcgcaac gcctggccgc gcggggccgc gagacgcccg
    21061 agcaaatcgc ccagcgcctg gcgcaggccg tgctgccggg cgatccggag gtgcctgcgc
    21121 cgggcgtgct gcacaagctg cccaacaacg cggcgcccga ggcagcggcg cagggcttgc
    21181 tggacatcgc ccacgcattg ctggccttgg tgcaataaaa aacgaggccg ttttttgcct
    21241 cgcctgcggc caagggctga tatttcctca ggccttcagc agcctttcca ccagcggcag
    21301 gtagtgcgac agcggcgggg tggcggcgtc gacctccttg gccaggtcgt cgtagcggcg
    21361 caggcgcacc gcggcttcgg catgcggctg ggtgatgaag gcctcggcgc cggcatcatc
    21421 gaaagggccg ccttgcaggg ccaggctgtc tttcgaggct tgcgacagcg tgccgtaata
    21481 ggcggcatcc acagcgcaca ggtagcgctt ggccgccacg tgcaggcgga tgggttcgat
    21541 gacctcgggg cccatcagcg gggccagggc ctgggcggcg acttcctggt gcctcatgtc
    21601 ctggatcctg ctcgacgcct gcagcaggtg gccgaggtcg tgcagcagcg cggcggcgat
    21661 cagctcgggc gcttcgccgg cctgttcggc caggcgggcg cattgcagcg cgtgctcggt
    21721 ctgggtgatg gcttccgagc cgtaaaaggc cgcgccgtag ccgtcgaaaa gttccgtgat
    21781 ccgcgtcagg gtgaccccgg ggagggtggt ttgcatggct gcgtgctccg gtgtaaaggc
    21841 tttagatcag ggccttgcgc aagcgggccg agagggtgtc gagggcaacc acggcgatga
    21901 tgatgatgat catgaccgcg caagtctggg catactggaa gctgcggatg atttcccaca
    21961 gcaccatgcc gatgccgccg gcgcccacga tgcccagcac cgaggccgag cgcacattcg
    22021 attcgaagcg gtacagcgac agcgacaccc acatgggcat cacctgcggc agcacgccgt
    22081 agatgatttc ctcgagcgca ttggcgcccg tggcgcgcac gccttccacg gggcgggggt
    22141 cgatggcctc gaccgattcg gagaacagct tggccagcac gcccatggtg tgcacccaca
    22201 gggccagcac gccggcgaag ggccccaggc ccacggcggc gatgaacagc atggcgaaca
    22261 ccatttcatt gatggcgcgg caggcgtcca tgaggcggcg catgggctgg tagacccagg
    22321 ccgggacgat gttcgagtag cacagcaggg cgaagggcac cgcgcagaca atggccagga
    22381 acgtgcccca gatggcgatc tggatggt
//
LOCUS       DASWPO010000051         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_10595, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000051 DASWPO010000000
VERSION     DASWPO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10595"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGC69_03120"
     CDS             <1..385
                     /locus_tag="VGC69_03120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0294309.1"
                     /translation="RWMDNDVYRHVNNVVYYSFFDTAVNRLLIEAGLLDVAESPVIGL
                     VVETGCRYFAPVAFPDEIHAGVRVARLGRSSVRYEIGLFRNDEDKASAAGHFVHVYVD
                     RATTRPTPIPDATRAFLASLQVEGK"
     gene            complement(504..1274)
                     /locus_tag="VGC69_03125"
     CDS             complement(504..1274)
                     /locus_tag="VGC69_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY0294310.1"
                     /translation="MAFVPDTRLYGTGTAATAATVDAGLRAYMLRVYNWMTSGLLLTG
                     IVAYGVANTSLINLFYPVVETPYGLRHTQGPLAIVAMLAPLAFVLVLSFGVNRLSKTA
                     VQGIFWAFCAAMGASLTSIFLAYSGESIVRTFFITAATFGGMSLWGYTTRRDLTGMGS
                     FLMMGLFGMILASLVNMFVGSSGLQFMISIVGVLVFTGLTAYDTQRIKSTYTQYAYAE
                     GPDQAGKRSVYDALSLYLNFINLFMLLLQLTGNRSNNN"
     gene            complement(1418..>1677)
                     /locus_tag="VGC69_03130"
     CDS             complement(1418..>1677)
                     /locus_tag="VGC69_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294311.1"
                     /translation="TRAQIRAAWLVEFGAIGAAAGLMAAAIGTLASWGVMHFIMQAPW
                     SFLPGLLAATVLGCVVLMLGFGFAGTEQALRARAAPYLRNE"
BASE COUNT          303 a          520 c          557 g          297 t
ORIGIN      
        1 gcggtggatg gacaacgacg tctaccggca cgtgaacaat gtcgtttact attccttctt
       61 cgacacggcg gtgaaccggc tgctgatcga agccgggctg ttggacgtgg ccgagagtcc
      121 ggtgatcggc ctggtcgtcg aaaccggctg ccgctatttc gcgccggtcg cctttccgga
      181 cgagatccat gccggggtgc gcgtggcccg gctgggccgg tcgagcgtgc ggtatgagat
      241 cggcctgttc cggaacgacg aggacaaggc ctcggcggca gggcatttcg tgcatgttta
      301 tgtggatcgc gcgacgaccc ggccaacgcc gatcccggat gctacgcggg cgtttttggc
      361 gtcgctgcag gttgaaggga agtaaggcca gggcgctgcc ctggacccgc aaaaggccgg
      421 aggccctttg aaccctttag tttaggattt caaaggctcc gcctttgatg gggtccaggg
      481 gcaacgcccc tggccttcct tcgttagttg ttattgctgc ggttgcccgt cagttgcagc
      541 agcagcatga acaggttgat gaagttcagg tacaggctca gcgcgtcata gacgctgcgc
      601 ttgcctgcct ggtccggccc ttccgcgtag gcgtattgcg tgtaggtcga cttgatgcgc
      661 tgcgtgtcgt aggcggtcag gccggtgaac accagcacgc ccacgatgct gatcatgaac
      721 tgcagcccgc tgctgccgac gaacatgttc accaggctgg cgaggatcat gccgaacagc
      781 cccatcatca ggaagctgcc catgcccgtc aggtcgcgcc gggtcgtgta gccccacagg
      841 ctcatcccgc cgaaggtggc ggcggtgatg aagaaggtac gcacgatcga ctcgccgcta
      901 taggcaagga agatcgacgt caggctggcg cccatggccg cgcagaacgc ccagaagatg
      961 ccctgcacgg cggttttgga caggcggttc acgccgaagc tcagcaccag cacgaaagcg
     1021 agcggcgcca gcatggccac gatcgccaac ggcccctgcg tgtggcgcag gccgtaaggt
     1081 gtttccacca cgggatagaa gaggttgatc aggctggtgt tcgccacgcc ataggcgacg
     1141 atgccggtca gcagcagccc gctggtcatc cagttataga cgcgcagcat gtaggcgcgc
     1201 agcccggcat cgacggtcgc ggcggtggcg gccgtgccgg tgccgtaaag gcgggtgtcg
     1261 ggaacgaagg ccatggccta ggggtcctta gatgtgaggc gatatgcccg tgtaacacat
     1321 cttgtccatc accgggacgc gatcaaggga attcccggcc gggttgcgcg caattcatgt
     1381 actacgcggg cagtagccag tttcctttat gactccttta ctcgttacgc agatagggcg
     1441 ccgcccgcgc ccgcagcgcc tgctccgtgc cggcgaagcc gaagcccagc atcagcacca
     1501 cgcagcccag caccgtggcg gccagcaggc ccggcaggaa cgaccaaggc gcctgcatga
     1561 tgaaatgcat cacgccccag cttgccaggg tgccgatcgc ggccgccatc agtccggccg
     1621 ccgcgccgat cgcgccgaat tccaccagcc aggccgcgcg gatctgcgcc cgggtgg
//
LOCUS       DASWPO010000052         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2064371, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000052 DASWPO010000000
VERSION     DASWPO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064371"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGC69_03135"
     CDS             complement(<1..248)
                     /locus_tag="VGC69_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0294312.1"
                     /translation="MFPKCEDGGKLILRLVLGLLILCHGISKMHYGIGFVADLLAKHN
                     LPSAIGYLVYIGEVVAPLMIILGILGRLGALIVVVNMIV"
     gene            complement(366..1208)
                     /locus_tag="VGC69_03140"
     CDS             complement(366..1208)
                     /locus_tag="VGC69_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HEY0294313.1"
                     /translation="MPRVSASLALLALLLTACSSDKPKAVYEEEDFTQNHTYSRSFES
                     DYKVACDAGRRALLSQGYNIDKSASDTVSGHKNFMDATGRNTQITFNVNCAPDSTGPS
                     HSQVFANAVQDSYTLKKSSTAASVGVGILGSVSLPFSSQDDSLVKTSTLTVTRPQFYG
                     GFFDLLRQYLAVAQLEQPEAPKKKPTPAPVPALVTPTVPLTPENTGIAKPPVEPAPQA
                     PAPKTDAAPASPEPAAEAPAAPALAPAPAATQPAAAPAEGVAAPSSADKPAAAPPAAD
                     KPAE"
     gene            complement(1304..2251)
                     /locus_tag="VGC69_03145"
     CDS             complement(1304..2251)
                     /locus_tag="VGC69_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294314.1"
                     /translation="MPLTRYTLRQFEAFTAVAESLGFATAGKRLGLSASAVSQLIAEL
                     ESLLGFKLFERTTRRVELSVAGRSYLPSVLAVLRQVRQAELDAEALNNKSTSIIKIAA
                     PQLMVSALLPAVIHAYMRDNPNAVVRLVDATVDTLFERIQNGEADVAVGPGIAATASS
                     VQRDVLFQDKWALWCAPDSPIVDLETVGWAQLRETRMVTSWKDPAFEVARRQHTDAEI
                     RALMPIELVDNISTAFGLARIDRSVVLAPTYVSNVATAMGLVHKPLVDPEIDCVVYLF
                     RPGGAMATQAAQAFGNFLLAWMRANAGAFHGPPAQQATT"
     gene            complement(2479..3243)
                     /locus_tag="VGC69_03150"
     CDS             complement(2479..3243)
                     /locus_tag="VGC69_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY0294315.1"
                     /translation="MNATPAFPARRRGPARLIILALMTLVLFAGFCSLGVWQIQRRAW
                     KLDLIAKVDQRVRQPAVEAPGLPQWPAVNAADDEYRHVRAEGTLRYGQETLVQAVTDY
                     GSGYWVLTPLQRGDGELVLVNRGFVLPEWRKRADHAEPAGPVSVAGLLRMDEPKGALF
                     RQNEPAIDLWYTRDLQAIAAKRGLARVAPYFIDAQAGPGAGKPDPAVAPVPGLTVVRF
                     PNNHLTYVITWFALAAMTLLGAALFWREERRRPAAG"
BASE COUNT          604 a         1055 c         1147 g          582 t
ORIGIN      
        1 acgatcatgt tgacgacgac gatcagcgcg cccaggcgac ccaggatgcc caggatgatc
       61 atcagcggcg cgacgacctc gccgatgtag accaggtagc cgatggcgct gggcaggttg
      121 tgcttggcca gcaggtcggc cacgaagccg atgccgtagt gcatcttgga gatgccgtgg
      181 cacaggatga gcaggcccag gaccaggcgc agaatcagtt tgccgccgtc ttcacattta
      241 ggaaacatga gattgactct ctcgagagtt gaaaaaagtg acgcgcatta tggccaatat
      301 atccggccgc aaatagcctc atcgacggaa caatccgtct caaccctcgt attgctggat
      361 cgatcttact cggcgggctt gtcggccgcg ggcggggccg ccgccggctt gtcggcgctc
      421 gagggggcgg cgacgccctc cgcaggcgcc gcggcaggtt gcgtggcagc cggcgcgggt
      481 gccagtgccg gtgcggccgg ggcttccgcc gccggctcag gcgatgccgg cgcggcatcg
      541 gtcttgggcg cgggcgcctg gggagccggt tcgacgggcg gcttggcaat gccggtgttc
      601 tccggcgtca gcggcacggt gggcgtgacc agggcgggca cgggcgccgg ggtcggcttc
      661 ttcttgggcg cctcgggctg ttcgagctgg gccacggcca ggtattgccg cagcaggtcg
      721 aagaagccgc cgtagaactg cggccgcgtc acggtgaggg tgctggtctt gaccagcgag
      781 tcgtcctgcg acgagaaggg cagggacacg ctgcccagga tgcccacccc cacgctggcc
      841 gccgtgctgg acttcttgag cgtgtagctg tcctgcacgg cattggcgaa cacttgcgaa
      901 tggctcggac cggtgctgtc gggggcgcag ttgacgttga aggtgatctg cgtgttgcgg
      961 ccggtggcat ccatgaagtt cttgtgcccg gagaccgtgt cgctggcgga cttgtcgatg
     1021 ttgtagccct ggctgagcaa agcgcggcgc cccgcgtcgc aagccacttt gtagtcggac
     1081 tcgaacgagc gcgagtaggt gtggttctgg gtgaagtcct cttcctcgta gaccgccttg
     1141 ggcttatcgg acgagcaagc ggtgagcaga agggcgagca aggcaagcga tgcggaaact
     1201 cggggcatgt cgattcgatg ggtagcggca ggcactgctg caagtcgact aatttaccaa
     1261 gtttccagac ccagcaagcc gcgcccccgg catcgaggcc gtttcatgtg gttgcttgtt
     1321 gggccggcgg accgtgaaaa gcgccggcat tggcgcgcat ccaggccagc agaaagttgc
     1381 cgaacgcctg ggcggcctgc gtggccatgg ccccgcccgg acggaacaga tacacgacgc
     1441 aatcgatttc cggatcgacc agcggcttgt gcaccaggcc catggccgtg gccacgttcg
     1501 agacataggt gggcgccagg accacggagc ggtcgatgcg cgccagtccg aaggccgtgc
     1561 tgatgttgtc gaccagctcg atgggcatca gcgcccggat ctcggcgtcc gtatgctggc
     1621 ggcgcgccac ttcgaaggcc gggtccttcc aggaggtgac catgcgcgtc tcgcgcagct
     1681 gcgcccagcc gaccgtctcg aggtcgacga tgggcgaatc gggcgcgcac cacaaggccc
     1741 acttgtcctg gaacaggacg tcgcgctgca cgctggaggc ggtggcggcg atgccgggcc
     1801 cgaccgccac gtcggcctcg ccgttctgga tgcgctcgaa cagcgtatcg accgtggcgt
     1861 ccaccaggcg aacgacggcg ttggggttgt cgcgcatata ggcgtggatg acggccggca
     1921 gcagagcgct gaccatcagt tgcggcgcgg cgatcttgat gatgctcgtg gacttgttgt
     1981 tgagcgcctc ggcatcgagc tcggcctgcc tgacctggcg cagcacggcc agcaccgagg
     2041 gcaggtagct gcgccccgcg accgaaagct ccacgcggcg cgtggtgcgt tcaaacagtt
     2101 tgaacccgag cagggattcg agttcggcga tgagctggct gacggcggag gcgctcagtc
     2161 ccaggcgctt gcccgccgtt gcgaacccca gtgattcagc caccgcagta aatgcttcaa
     2221 attgcctcag ggtatatcgt gtcagtggca ttcaacttac tattattatg aaaatgcaaa
     2281 taaacaaccc cgtttattcc attaatcgca aggcattaaa ccaggggcat gctcctggat
     2341 atatcggcta catatccctc atacttaaca gtactttaac tatatgtatc actgccatga
     2401 caaaaatggg tcattaaatt tttgaagcaa tagtgcgcta tccggcacta aatctttttt
     2461 atgcccccgt atttcgcatc aaccggccgc tgggcggcgg cgctcttcgc gccagaacag
     2521 ggccgcgccc aaaagcgtca tcgcggccag ggcaaaccag gtgatcacat aggtcaggtg
     2581 gttgttgggg aagcgcacga cggtcagccc cggcaccggc gcgacggccg ggtcgggctt
     2641 gccggctccc ggtccggcct gcgcgtcgat gaagtaaggc gccacccgcg ccaggccgcg
     2701 cttggcggcg atggcctgca ggtcgcgggt gtaccagagg tcgatggccg gctcgttctg
     2761 ccggaaaagg gcgcccttgg gttcgtccat gcgcagcagc ccggccaccg agaccggtcc
     2821 ggcgggttcg gcgtggtccg cgcgcttgcg ccactcgggc agcacgaaac cccggttgac
     2881 cagcaccagt tcgccgtcgc cgcgctgcag gggcgtcagc acccagtagc cgctaccgta
     2941 gtccgtgacc gcctgcacca gggtttcctg gccgtagcgc agcgtgccct cggcccggac
     3001 atggcggtac tcgtcgtcgg ccgcattgac ggccggccat tgcggcaagc ccggcgcctc
     3061 gacggccggc tggcgcaccc gctggtcgac cttggcgatc aagtccagct tccaggcccg
     3121 gcgttgtatc tgccagacac ccagggagca gaagccggcg aacagcacca gcgtcatcag
     3181 ggcaagaatg atcaagcgcg ccgggccgcg gcgccgggcg ggaaaagcag gggtagcgtt
     3241 caaaaccagt cctacgggaa tcggccgatc aaggagtctt cggccacatc cagtagcgta
     3301 ccgccactga caacggccag gccagcgcca gccacgccgc ccagcgccag atgcccgtgc
     3361 cgagcaaggc cgccaacagg cccgacat
//
LOCUS       DASWPO010000053         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_547219, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000053 DASWPO010000000
VERSION     DASWPO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547219"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VGC69_03155"
     CDS             complement(<1..784)
                     /locus_tag="VGC69_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY0294316.1"
                     /translation="MTILVIAEHDNAQLKGATLNTVAAAAKIGGDIHVLVAGSQAQAV
                     AEAATQIAGVAKVLLADAPQLADGLAENLEAQVLAVAQANGYSHILFAATASGKNVAP
                     RVAAKLDVAQISEIIGVVSPDTFVRPIYAGNAFATVQSADPVKVITVRTTGFDAQAAT
                     GGSAVVESVTATPDSGLSTFIGREVAKSDRPELAGAKVVVSGGRGMGSAENFKILEPL
                     ADKLGAAMGASRAAVDAGYAPNDWQVGQTGKIVAPQLYVAVGI"
     gene            complement(787..1536)
                     /locus_tag="VGC69_03160"
     CDS             complement(787..1536)
                     /locus_tag="VGC69_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0294317.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDESGVDIANVKMSMNPFDEIGVE
                     EATRLRESGKATEVIAVSCGVSQAQETLRTALAIGADRGILVQTDAELQPLAVAKLLK
                     GVAEREQPQLIILGKQAIDDDANQTGQMLAALLGWPQATFASKLELGEGEVTVTREVD
                     GGLETVKLKLPAIVTTDLRLNEPRYVTLPNIMKAKKKPLETVTPADLGVDPSPRLKTL
                     KVSEPPARKAGIKVADVAALVDKLKNEAKVV"
     gene            complement(1592..>2372)
                     /locus_tag="VGC69_03165"
     CDS             complement(1592..>2372)
                     /locus_tag="VGC69_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0294318.1"
                     /translation="AATREAILRVHAASYLDSLQSRLPRDGGYTAIDPDTLMNRHTLE
                     AALHAAGAAVAAVDAVMTGQAETAFCAVRPPGHHACRDRAMGFCFLNNVAIGAAHAMA
                     VYGVQRLAIIDFDVHHGNGTEQAFSGDERVLMCSFFQHPFFPNSGVDQPAANMLNVPM
                     PAYTAGQAVRALVTERWLPRLEAHRPELILVSAGFDAHREDDMAQMGLVEADYAWMTE
                     RIVDVAGRYAGGRIVSTLEGGYSLSALGRSVVAHIRALSRL"
BASE COUNT          356 a          885 c          724 g          407 t
ORIGIN      
        1 agatccccac cgccacgtac agctgcggcg ccacgatctt gccggtttgc cccacctgcc
       61 agtcgttggg cgcgtagccc gcatccaccg ccgcgcgcga ggcccccatc gccgccccga
      121 gcttgtcggc caagggctcc aggatcttga aattctccgc gctgcccatg ccccggccgc
      181 cagacaccac caccttggcg cccgccagtt cggggcggtc gctcttggcc acttcccggc
      241 cgataaaggt cgacaagccc gaatccgggg tggcggtaac gctttccacc accgccgaac
      301 cgcccgtggc cgcctgggca tcgaagcccg tggtgcgcac cgtgatcacc ttgaccggat
      361 cggcggactg caccgtggcg aaggcgttgc ccgcatagat cggacgcaca aaagtatccg
      421 gggacacgac cccgatgatc tcggagatct gcgccacgtc cagcttggcc gccacccgcg
      481 gggccacgtt cttgcccgag gccgtggctg cgaacaggat gtggctgtag ccgttggcct
      541 gggccaccgc cagcacctgg gcctcgaggt tctccgccag gccgtcggcc agttgcggcg
      601 catcggccag cagcaccttc gccacccccg cgatctgcgt ggccgcctcg gccaccgcct
      661 gcgcctggct gcccgccacc agcacgtgga tgtcgccgcc gatcttggcg gccgcggcca
      721 cggtattcag ggttgcgccc ttcagttggg cgttgtcgtg ttcggcaata accaggatcg
      781 tcatcttcac accaccttcg cttcgttctt gagcttgtcc accagcgccg ccacgtcggc
      841 caccttgatg ccggccttgc gcgccggcgg ctcgctcacc ttcaaggtct tcaacctcgg
      901 ggacggatca acccccaggt ccgccggcgt caccgtctcc agcggcttct tcttggcctt
      961 catgatgttg ggcagcgtca cgtagcgcgg ctcgttcaga cgcaggtcgg tcgtgacgat
     1021 cgccggcagc ttgagcttga ccgtctccag gccgccgtcg acctcacggg tcaccgttac
     1081 ctcgccctcg cccagctcca gcttgctcgc aaaggtcgcc tgcggccagc ccaggagcgc
     1141 ggccagcatc tggcccgtct ggttggcgtc gtcgtcgatc gcctgcttgc ccaggatgat
     1201 cagctgcggc tgctcgcgct cggccacccc cttcaacagc ttggccaccg ccagcggctg
     1261 cagctccgca tcggtctgca ccagaatgcc ccggtccgcc ccgatcgcca gcgccgtgcg
     1321 cagcgtctcc tgcgcctgcg acaccccgca cgacaccgcg atcacctccg tcgccttgcc
     1381 cgactcccgc agccgcgtcg cctcctccac cccgatctca tcgaacggat tcatcgacat
     1441 cttcacgttc gctatgtcca caccgctttc atcgctcttc acgcgtacct tgacgttgta
     1501 gtcaaccacg cgcttgacgg gtactagaac cttcatccaa caatctcctg gagcacttcc
     1561 ctgatacggg attttgagta atttttcgat tctacaacct agacagcgcc cggatgtggg
     1621 ccaccacgct gcggcccagg gccgacaggc tgtagccgcc ctcgagcgtg ctgacgatgc
     1681 gccccccggc gtaccggccg gccacgtcca cgatccgttc ggtcatccag gcgtaatcgg
     1741 cctcgaccag gcccatctgg gccatgtcgt cttcacggtg cgcatcgaaa ccggccgaca
     1801 ccaggatcag ctcgggccgg tgcgcctcga gccgcggcag ccaccgttcg gtgacgagcg
     1861 cgcgcacggc ctgcccggcc gtgtaggccg gcatcggcac attgagcata ttggccgcgg
     1921 gctggtcgac cccgctgttg ggaaagaacg ggtgctggaa aaaactgcac atcagcaccc
     1981 gctcgtcgcc cgagaacgcc tgctcggtgc cattgccgtg gtgcacgtcg aaatcgatga
     2041 tggccaggcg ctgcacgccg tacacagcca tggcgtgcgc ggcgccgatg gccacgttgt
     2101 tcaggaaaca gaagcccatg gcgcggtcgc ggcaggcatg gtgaccgggc ggacgcacgg
     2161 cgcagaaggc cgtctcggcc tggccggtca tcacggcatc caccgccgcc accgcggcgc
     2221 cggccgcatg cagggccgcc tccagcgtgt ggcggttcat cagcgtgtcg gggtcgatgg
     2281 cggtgtagcc gccgtcccgc ggcaggcgcg actgcaggga gtcgagatac gaggcggcgt
     2341 gcacgcgcag gatggcctcg cgcgtggccg cg
//
LOCUS       DASWPO010000054        35870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1081821, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000054 DASWPO010000000
VERSION     DASWPO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 35870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35870
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081821"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGC69_03170"
     CDS             <1..214
                     /locus_tag="VGC69_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294319.1"
                     /translation="RSTVLFVTHSITEAVLLSDTVVVMSARPGRIRDVVPVDLPRPRA
                     PDTLRDPRFLALSERVREGIEAQWVE"
     gene            219..995
                     /locus_tag="VGC69_03175"
     CDS             219..995
                     /locus_tag="VGC69_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294320.1"
                     /translation="MTRPIPKRMAAIAKPLIAFALMLLAWEIGAPRVGIPTYVLPLPS
                     MIAQRFAETFSVQMLGLAMTAFTTLAGLGLALVFGVLLALAIIYVPLLRSIVTPLLAA
                     FNSIPKIAIAPLFVIWFGLGYESKILLAFLLALFPIFVNSVTGLGEIEADLIDLSTLA
                     GGTTWRIFMKVRLMNAVPYIADAVKVAFPLALVGSIVGEFIGGNSGIGHLILSGQFNL
                     DTPLVFATLLSITLFTTLGIGAVSLFEHAFLTWRPSQRQR"
     gene            1034..2359
                     /locus_tag="VGC69_03180"
     CDS             1034..2359
                     /locus_tag="VGC69_03180"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY0294321.1"
                     /translation="MRDYLSRLRQRGDLLVVDKEIDPFHELAAVTHHAQKKWAKPILF
                     TNVKGTTFPVVTNVYGTRERLAEVIGIDANDFCRQWSNLASLGAAAMPEPLVPARAAP
                     DYVDVKLSDLPLITYSDRDGGPYFTSAMFIAKDPETGVGNLSYHRSMYVSDNELRCRL
                     APRHHLTIYHEKAEKMGKPLEAAMLIGAPSQAFLTAAAPLPYDVDELEVAARLRGSPI
                     EMRPCKHIDLEVPAETEIVIEGRFLPNERRPEGPFGEFMGYYVPVGPNAVFEVLGVTA
                     RKDAMFHSILCGSPEEVLTLELSVSANIYQRLSAALPGIVNVTCQPFVNHAIIQIDPQ
                     FEGHARQVMLATIGAEPIWAKIITVVDRDVDIYDMDDVMWAILTRSRPDKDTLIIPDT
                     PSFYRDEFKDHWGRLLIDATKPWGREKEFERKRLRRVNDVRLSDWFKEA"
     gene            2359..2937
                     /locus_tag="VGC69_03185"
     CDS             2359..2937
                     /locus_tag="VGC69_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY0294322.1"
                     /translation="MAQAQRLVIGITGASGAVYGVRALELARGAGVETHLIVSRSALL
                     TLNQELGLQKEDLAARADVMHPVQDIGATISSGSFKTMGMLIAPCSIRTMSEIASGVT
                     STLISRAADVVLKERRKLVLMVRETPLHLGHLRSMTQLTEMGAIIMPPVPAFYAKPDT
                     LDDMVNHTVARALDLFGIETDAVKRWGGLKAE"
     gene            complement(2969..4429)
                     /gene="tldD"
                     /locus_tag="VGC69_03190"
     CDS             complement(2969..4429)
                     /gene="tldD"
                     /locus_tag="VGC69_03190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY0294323.1"
                     /translation="MKLIDPAIQSLATAKSLLLDPWGLTEASLAHALGEIFTHKVDYA
                     DLYFQYTRSEGWSLEEGIVKTGSFSIGQGVGVRAVSGEKTAFAYSDSLSPEALMSAAH
                     TVRGIARRGAGKLKVSAQVPPDDARSLYTDIDPLATLSAAEKVVLLERIERMARAADP
                     HVIQVMAGLGAEYDVVLVAGSDGRLAADVRPLVRLSITVIAERNGRREMGHGGGGGRT
                     GLEMFTDEVMQEYVDRAVHEAMVNLEARPAPAGEMTVVLGSGWPGILLHEAVGHGLEG
                     DFNRKGSSVFCGRIGQRVASKGVTVIDDGTLPGRRGSLNVDDEGNATQHNVLIEDGIL
                     RGYMQDTLNARLMKTGATGNGRRESFAHLPMPRMTNTYMLAGTTPPEEIVASVKRGLY
                     AVNFGGGQVDITSGKFVFSASEAYMIENGKVTYPVKGATLIGNGPDAMNRVSLIGNDL
                     RLDSGVGTCGKEGQSVPVGVGMPTVRMDGLTVGGTA"
     gene            complement(4502..5317)
                     /locus_tag="VGC69_03195"
     CDS             complement(4502..5317)
                     /locus_tag="VGC69_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY0294324.1"
                     /translation="MTEIFSPFQIAAVQMVSTPEVSENLEAAGRLIAEAAGSGAKLVA
                     LPEYFCLLGRRDTDKLAVKETFGSGPIQDFLARTAERHGIWLAGGSMPLDCPDPNKVY
                     NTTLVFGPDGKCKARYDKIHLFSFKRGAEAYDESVAIQPGGDIQIFEAPCGPVGLSIC
                     YDLRFAELYRNMGPVNLILVPSAFTYTTGQAHWETLLRARAIENQCYVLAPAQGGTHP
                     NGRRTWGHSMLIDPWGQIIDVLPEGPGVIGGTIEPERLSDVRAALPALRHRVL"
     gene            5511..5888
                     /locus_tag="VGC69_03200"
     CDS             5511..5888
                     /locus_tag="VGC69_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEY0294325.1"
                     /translation="MARVTYASQQAKIEKEITKLQKQAAALQSKRRKPIIASIVKSMR
                     EYDITPEDIVATYNSTKPGRGAKRKVASGSASTASAAAKRAVAPKYRHPETGETWSGR
                     GKAPRWLMAAEADGASRDSFLIA"
     gene            6223..9141
                     /gene="pepN"
                     /locus_tag="VGC69_03205"
     CDS             6223..9141
                     /gene="pepN"
                     /locus_tag="VGC69_03205"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY0294326.1"
                     /translation="MRTDMPVTIYRKDYRPYPYAIPQVALAFDLDPDVTTVHCAMQVE
                     RRPDAPADAPLEFDGEALELVSLRVDGQPWPAAQYTVDEHSLVIRGLPALARVETISR
                     CRPAANSTLMGLYVSGDNFFTQCEAEGFRRITWFADRPDIMSRYRVTLRARPDYPVLL
                     SNGNLIAERELPDGRREAEWEDPFPKPCYLFALVAGKLTHRETRIKTMSGRDVLLQVY
                     SDPGSESRTQWALDSLVRAVHWDEKRFGLELDLDRFMVVAVRDFNMGAMENKGLNIFN
                     AAYVLADPDTATDANYEGIESVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEF
                     SADMMAREILSAPTLRPAGLLPPEGARPPWGGPAAGCAAAAASARTVKRIDDVVTLRA
                     AQFPEDAGPMAHPIRPDSYQEIGNFYTATVYEKGAEVIRMQHTLLGEAGFRAGMDEYF
                     RRHDGQAVTCDDFVAAMESVYVRQHPGRDLQQFRNWYRQAGTPRVSVALGYDPAARRC
                     TLTLSQSCPPVGVERRPGQPAKQPLHIPFAIGLLDRTGKPMPLALDDQPPTGTVLLEL
                     TGERQQWVFDGIPSAPVPSLLRGFSAPVIVDYAWTDEELALLSAHDTDAFARWEAGQE
                     LATRQILALTRQHQAGQPMAVADAFIEAWRALLTDPALDAAYRARTLLLPSEKTLAER
                     MEGSVPQGGDSVLGRPGDGSGPHAAPFGLAAPQGGDSVLGRPGDGMDPPALAAARDHL
                     RAELGRRLAAEWRAAFDANQTPGAYSPAPGPAGMRALKNQALAHLMAGGIADAQALAE
                     RQYATAGNMTDSMAALSSLINYGAGDAPARALAAFYERWQGDPLVVDKWFTLQATARA
                     TDVAAVRALMQHPAFTLRNPNRARALVFQFCLNNARGMHRPDGLGYAYWADQVIALDG
                     LNPEVAARLARGLDSWSRFVPPLRDPMRQALERVAGHPGLSRNVLEIVSKALEFAAQA
                     "
     gene            9152..10168
                     /locus_tag="VGC69_03210"
     CDS             9152..10168
                     /locus_tag="VGC69_03210"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY0294327.1"
                     /translation="MKNKTLTQYLVEQQRSAQLLQPEVRLLIEVVARACKSIGHAVGK
                     GALGGVLGALETENVQGEVQKKLDVLSNEILLEANEWGGHLAAMASEEMETIHPIPNR
                     YPKGEYLLLFDPLDGSSNIDVNVSIGTIFSVLKAPHAVGEAVTEQDFLQPGARQVAAG
                     YAVYGPQTMLVLTVGNGTVGFTLDREMGSWVMTQEKYVIPEDTKEFAINMSNMRHWAP
                     PMKRYIDDCLAGKTGPLGKDYNMRWIASMVADVHRVLTRGGIFMYPWDTREPSKPGKL
                     RLMYEANPMSLLVEQAGGKAITGTQRILEVQPEKLHQRVSVILGSKNEVERVGRYHAE
                     DAGK"
     gene            complement(10337..11749)
                     /gene="thrC"
                     /locus_tag="VGC69_03215"
     CDS             complement(10337..11749)
                     /gene="thrC"
                     /locus_tag="VGC69_03215"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0294328.1"
                     /translation="MTNYVSTRGGMAPQSFSDILLEGLAPDGGLAVPQDLPRVDAAML
                     ESWRGLAYADLAFEVLSRFVTDVPADDLRKLVRAAYRPATFKSAEIVPVKPLNTSMAL
                     LGLSEGPTLAFKDMAMQFLGQIFEYVLAKRGATLNIVGATSGDTGSAAEYALRGKQGV
                     AVFMLSPHGRMSAFQRAQMYSLQDENIHNIAVRGVFDEAQDIVKALAGDLDFKRRVHL
                     GAVNSINWARISAQVVYYFWGWLRATTGAGQQVSFTVPSGNFGNILSGHIARGMGLPV
                     RRLVLATNENNVLEEFFRTGIYAPRNPEHTYATSSPSMDISRASNFERFVHDLVGRDG
                     ARVKALWAELAETGRFDLSSEKARFESEFGFVAGQSSHADRLAAIRATLESTGVLIDP
                     HTADGVKVAAQYVEPGIPMLVLETALPAKFSETIQQAIGRPAPVPATLAGLESLPQRV
                     EVMDCNVQSVRHYIESHAKM"
     gene            complement(11790..13094)
                     /locus_tag="VGC69_03220"
     CDS             complement(11790..13094)
                     /locus_tag="VGC69_03220"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY0294329.1"
                     /translation="MGPIKVGLVGLGVVGGGTWTVLARNAEEIARRAGRRIEVARIAV
                     RDVAKARSKVGEAVPVSTDVHGLVRDPGIDIVVELIGGDTLARELVLEAIAHGKHVVT
                     ANKALLAKHGNEIFAAASERGVMVAFEAAVAGGIPIIKAIREGLTANRIEWLAGIING
                     TTNFILSEMRSRGLPFDTVLAEAQRLGYAEADPTFDVEGVDAAHKLTLLASIAFGVPV
                     QFDKAHIEGISRLESADIAHAERLGYRIKLLGITRRRADGIELRVHPALVPADKLLAN
                     VEGAMNAVLVRGDAVGPTLYYGQGAGEEPTASAVVADLVDITRLHTADPGNRVPHLAF
                     QPDSMSDLAILPMDRVQTSYYLRLRVDDVPGVLADIARILADRGISIGSMIQQPSNLG
                     GADIIFLTHEAVEGKVNQAIAAIEVLPFVRSKVTRLRIENLQ"
     gene            complement(13127..14335)
                     /gene="alaC"
                     /locus_tag="VGC69_03225"
     CDS             complement(13127..14335)
                     /gene="alaC"
                     /locus_tag="VGC69_03225"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEY0294330.1"
                     /translation="MSGSDKPARTFTRIERLPPYVFNITAELKMAARRRGEDIIDMSM
                     GNPDGPTPRHIVEKMVEATLRPTTHGYSVSKGIPRLRKAICDWYQRRYAVEFDPDSEA
                     IVTIGSKEGLAHLMLATLDRGDTVLVPNPSYPIHIYGAVIAGANIRSVRMTPGVDFFE
                     ELERAVRESIPKPKMMVLGFPSNPTAQCVDLSFFERVVALAKEHDILVVHDLAYADIC
                     FDGYVAPSIMQVPGARDVAVEFFTMSKSYNMAGWRIGYMVGNKDLVNALARIKSYHDY
                     GTFTPIQVAAIAALDGPQDCVDEIVAQYQSRRDVLARGLIEAGWNVDIPRASMYIWAH
                     IPEPYRAMGSLEFAKHVLAEARVAVSPGIGFGEYGDEYVRFALIENEQRTRQAVRGIK
                     DMFRKDGLLK"
     gene            14517..14999
                     /locus_tag="VGC69_03230"
     CDS             14517..14999
                     /locus_tag="VGC69_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEY0294331.1"
                     /translation="MKLHNDPATTLNTVTAYGPDYIEVNQVRFSHAVAFGPEGAVAQW
                     PVESPADITSALLQQAAGLSQASRDPLAFLDDPESAAGGMAGVRPPDAPEVLLVGTGA
                     RQKMLPPAVLRPILAVGVGIEVMDTQAAARTYNILMAEGRRVIVALLPTNVPTHGESA
                     "
     gene            14999..15463
                     /locus_tag="VGC69_03235"
     CDS             14999..15463
                     /locus_tag="VGC69_03235"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0294332.1"
                     /translation="MSALIGKPAPQFVAESTIGTIDLSQCRGRAVVLYFYPKDNTPGC
                     TTEGQDFRDLHDQFLAASTVVLGVSRDTLKSHENFCEKHNLPFPLIADPDETLCNLYG
                     VMKQKNLYGKQVRGIERSTFLIDAYGLLVQAWRGVKVPGHAKEVLQAASSIG"
     gene            15542..17197
                     /locus_tag="VGC69_03240"
     CDS             15542..17197
                     /locus_tag="VGC69_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0294333.1"
                     /translation="MPLPKLPTRPAAILTFPMGETAPAASPAPRPAPPAALEPHTHQP
                     QLDGMEAAVVPIADAGASAAPIAARSPEPQAKPAPAAARKSKAKTQPAVRKLFVLDTN
                     VLLHDPSSLFRFEEHDIFLPMMTLEELDHQKKGMSEVARNARQVSRSLDSLVQNATDL
                     DLGVDLTTLGNKDATGRLLFQTTAIHSTLPSDLPMGKADNQILGVVRALQEKYTQREV
                     VLVSKDINMRLKARALGLQAEDYYNDHVLEDTDLMYAGMMQLPEDFWNRHGKDVESWQ
                     QGGTTFYRIKGPLCAQFMVNQFVYFEGQMPLYAQVREVSGKTAVLATLRDYTHGKNNV
                     WGITARNREQNFALNLLMNPDIDFISLLGQAGTGKTLLALAAGITQVLETKRYTEIIM
                     TRVTVPVGEDIGFLPGTEEEKMLPWMGALEDNLDVLNMGEGDSGGDWGRAATMDLIRS
                     RIKVKSLNFMRGRTFLNKYLIIDEAQNLTPKQMKTLVTRAGPGTKVICLGNIAQIDTP
                     YLTEGSSGLTFVVDRFKGWPHAGHITLQRGERSRLADYAGDVL"
     gene            complement(17213..18187)
                     /locus_tag="VGC69_03245"
     CDS             complement(17213..18187)
                     /locus_tag="VGC69_03245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEY0294334.1"
                     /translation="MFKALVLDKEPEFHAAIRAVGDDFLPEGDVTIAVEYSTLNYKDG
                     LAITNRAPVVRNWPMVAGVDGAGTVMSSSHPAWQAGDRVLLNGFGVGEAHKGCLAQRA
                     RLKGDWLVRVPQAFTTRQAMAIGTAGYTAMLCVMALERHGVKAGDGEILVTGATGGVG
                     SVAVALLAKLGHCVVAATGKQAESGYLQSLGAARIIDRAELGAPGKPLQKERWAAVID
                     AVGSHTLANACAQTRYGGAVAACGLAQGPDFPATVMPFILRSVALLGVDSVMAPIALR
                     EEAWRRLAADLDPSLLAAITTEIALDEAVDAARRLMDGEVRGRIVVRL"
     gene            complement(18227..19006)
                     /locus_tag="VGC69_03250"
     CDS             complement(18227..19006)
                     /locus_tag="VGC69_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294335.1"
                     /translation="MRKFIFTLATLAAAALPLASQAQSDTLRFGVDPTFRPYEYRLAD
                     GSLAGFDVDLGNEICKRLNAKCMWVQQPFDSIIPALKAKKFDAILSGMTATPKRREQL
                     DFTDRLFAGIGALVAKCGTELHATPESLRGKRIGVGLGSSQEAYAKTHWAPKGVELVP
                     YPNMEGVRADLVAGRIDVSFESAAPAQIGFLDQPQGKGFCMVGEKITDKDLVGDGTAI
                     GLEKGNDALRTRINEAIAAIRKDGTYMTIAKKYFDYDPYGE"
     gene            complement(19058..19822)
                     /locus_tag="VGC69_03255"
     CDS             complement(19058..19822)
                     /locus_tag="VGC69_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294336.1"
                     /translation="MPKLNVRNLEKTFGTHQVLDGVSLQAGAGDVISIIGSSGSGKST
                     FLRCINFLETPSAGQIWVDDELIRTLPGEQGLRVADPRQLQKIRAKLSMVFQHFNLWA
                     HMTALQNVTQAPIHVLGLSRADAEERGRHYLAKVGLSAKVESQYPAHLSGGQQQRVAI
                     ARALAMEPEVMLFDEPTSALDPELVGEVLKVMQNLAEEGRTMIVVTHEMGFARNVSKH
                     VLFLHQGKIEEEGDAREVLERPKSVRLAQFLAGQLK"
     gene            complement(19835..20548)
                     /locus_tag="VGC69_03260"
     CDS             complement(19835..20548)
                     /locus_tag="VGC69_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294337.1"
                     /translation="MIGIIQEYWRNYLYSDGYHWTGVAVTLWLLVVSTVLGFVLAVPL
                     AVARASSRKWLRGPVWFYTYIFRGTPLYVQLLICYSGIYSLDFVRGTPFLSSFFRDGM
                     NCAMLAFVLHVCAYNVEIFAGAIKATPYGEIEAARAFGMTGPTLYCRVILPSALRRAL
                     PAYSNEVVMMLHATSLAFTATVPEILKVARDVNSATYQAFNAFGIAAVLYACLAFVLI
                     CGFRHMEARWLAYLKPLRH"
     gene            complement(20545..21246)
                     /locus_tag="VGC69_03265"
     CDS             complement(20545..21246)
                     /locus_tag="VGC69_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294338.1"
                     /translation="MAGFFSLDGYGRLLLEGTWATIELGVLSLVVAFLLGLLGASAKL
                     SRSRLLRAVATAYTTLIRAVPDLVLMLLLFYGIQIGLNHVTESLGLQQMDIPPFLAGV
                     ITLGFIYGAYYTETLRGAFLAVPRGQIEAGYAYGMSGWRVFRDILFPQMMRFALPGIG
                     NNWLVVVKASAIVTILGLDDLVKAAIDASQGTQRAFFFLVLAAGIFLAITTVSSLVLA
                     WAEKRYSLGVRESEL"
     gene            complement(21251..21961)
                     /locus_tag="VGC69_03270"
     CDS             complement(21251..21961)
                     /locus_tag="VGC69_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0294339.1"
                     /translation="MAQAVAQADADPQVRVVIVTGAGGKAFSSGYDMKETTAAPITTL
                     PQMRARMQKDIHFTYSVWDCSKPVIALIDGFCLAGALELAMCCDMRYCSEPSTFAALE
                     ARFSTGISTMIMPWLLGQRARALIYSGDTIDAAEAWRLGLVDKVFSKETVQAEVRKIA
                     PRMSRVSLECLQADKRALNQTFEIMGLRQAIEYGAEACTLMNFSGSEEKAKFMSIRKE
                     QGLGPALKWRSEQFAPYE"
     gene            complement(22063..24141)
                     /locus_tag="VGC69_03275"
     CDS             complement(22063..24141)
                     /locus_tag="VGC69_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0294340.1"
                     /translation="MRPRSIAIVGVSERPGAAGTRVWNNLRKLGFPGPVYPVNPKYET
                     FDGAACYPGLAALPETVDVVFLAVPAAAGPDLVEEAGRLGIGAAFLNANGYADGDEHG
                     KALQRRIVDSARRHGMALSGPNNLGLVNIHARTAMWTPMHMRMNKPGPVGLISQSGSV
                     AMALAEDERELGFAYLVTTGNEAGATVADYLDVMVRDDNVQVILLFLETVRDPALLAS
                     AAREAARRGKRILALKLGTSEGGRALVQAHTGSLAGEDRLYDAFFKHLGIVRVRDLDE
                     MLETASLFVSSCAAPPACGTAIMTLSGGEAALLADIGHDVGLAYPPLSPETLAKMRPA
                     FPPHSTVANPVDGWGLGFNEENFRIILRALMEDPGLGTLGFVVDAPGHGGCDTHFALI
                     MGRVCAEVLAKTASGKRLVFINNIAGTGVNAEVRAVLDPAGIAYLSGMPTALKAIRNL
                     VDAPVARPEARAGEPLAVLPGDEPGRFQALRHAGVPMTPSERVDDAASAADAAQRLGY
                     PVVLKGVADHLPHKSDLGLVRLGLRDAAAVGQAYAALRAILDEQALPGASGEIVVQKM
                     AGSGVELIVGVRNDPRFGSFVIAGPGGVLVEISKQAGMRLGPISAEEARAMLRETAAG
                     RLLDGVRGQGPWDIDAAAEAIAAFSRFGHAHRDTLAALEINPLIVQEHGAVGVDLLLE
                     PHTPAEENSI"
     gene            24254..25057
                     /locus_tag="VGC69_03280"
     CDS             24254..25057
                     /locus_tag="VGC69_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0294341.1"
                     /translation="MEIKPLIGALGVEVLGMDTSAPSDELVQQMRSLLWEYQVLVIRD
                     QKLSCEQHIALARKFGPVHAQPFNYAKALPNHPEILPVIKEKTDTKVFGEKWHADVTY
                     LQRPLLGALLYALETPPRGGDTLFANMYRAYDALSDGMKQMLAGLTAEHEGSVPDGDP
                     VTGERYEPIRLIHHHGEHPAITVHPETGKKLLYVNTTYTRRFKGMTDAESRPLLEYLC
                     NHATQAEFTARVHWKPGTLVMWDNRSTQHLPINDYYGYRRAMHRITVST"
     gene            25198..26034
                     /locus_tag="VGC69_03285"
     CDS             25198..26034
                     /locus_tag="VGC69_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294342.1"
                     /translation="MKSKPQHLLFRFLAGMTLATASHLAMAGVLEDAKARGELVACTE
                     FQFAPFEFLDGDKPVGFDIDLVALIAQDMGVKVKYIDLPWSSVLPALESKRCDLVLAG
                     VMRTKARMERYDMSLPIGDAIVALVKRHGDASIMKPEDIAGKVVGGVKGSAQIQIFQN
                     FIDTKVPGGVKELKAYTASTQSYADLAAGRIVVASAAMANLAYLVKTRPEFEIVEPGF
                     GPPTYFGFPIRKEPESQTLAAAINAGITKYNKDGTMAKLQEKWFGKAFTLPYENVPEP
                     TI"
     gene            26048..26725
                     /locus_tag="VGC69_03290"
     CDS             26048..26725
                     /locus_tag="VGC69_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0294343.1"
                     /translation="MVPTTGVTSFLTGIHLLLVGAGVTAGISIVAICLGFLFASLVCV
                     ACMSRSRPLRFAGEVYVSFFRGVPLLIQLLVLYYLLPFIGLDLPPVVAGTLGLALCTS
                     AYQAENLRGGLLTIPAGQEEAARAFGYNVVQRWTRILLPQALRLSMPMIVNEMIAILK
                     ASSLVSVVGITDLTRVSQNIVARNFQPIQWYATAALFYLAINLVLSYFSRVAGRRLGR
                     SHSGAML"
     gene            26722..27390
                     /locus_tag="VGC69_03295"
     CDS             26722..27390
                     /locus_tag="VGC69_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0294344.1"
                     /translation="MKLDFSLLLQFGPDLLQGFFNTIKIWLSAALLAALIGSVLGFAM
                     TVGPRRLRQAITCYVEFFRCTPFLVQLFLFYFGGPSIGLTLDAYPVGIIGLGLYGGAY
                     FTATMHAGFNSIPKGQIEAAQSFGISRWQTVRHVVLPQMLVLIIPPGINLLIFMIKDT
                     AVLSIITVPELTFQITNITQETYAFVEPYLALALGYWVLVEITARLGRLGESLATRHL
                     GKAA"
     gene            27387..28214
                     /locus_tag="VGC69_03300"
     CDS             27387..28214
                     /locus_tag="VGC69_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0294345.1"
                     /translation="MTASPNTAPQAASPLIVIEGLSKSYGDHPVLHDVSLTINRSEVA
                     CIIGPSGSGKSTLLRCMNFLEDFSAGTIHIDGKPLGYRKTPDGSRAATSRAALEQARG
                     GVAMVFQQFNLWPHMTVLQNVTQPLVLVHGVPAGRARETGMKMLERVGLAHKAQAYPG
                     NLSGGQQQRVGIARALAIDPSVILFDEPTSSLDPELVGEVLQVIKQLAKDGLTMVVVT
                     HEMGFAAQMADRAIFVDHGAIVEEGPPRTLFRNPKSERLRQFLQTWMERSQLFQDAP"
     gene            28259..29257
                     /gene="pdxA"
                     /locus_tag="VGC69_03305"
     CDS             28259..29257
                     /gene="pdxA"
                     /locus_tag="VGC69_03305"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY0294346.1"
                     /translation="MTRSQLSRPVGITMGDAAGIGPEIVVKAFAQGLAAPAIVYGDAG
                     ALRRAAALVGAPLAINEIHSPDAARCSAEAIDVVASSPALPADLPAGKVSAAAGQGSY
                     DYLCAAIDDAMAGRIRAIVTAPLNKYAMHLAGIDQPGHTEILAERSGTADYAMMLAND
                     ELRVLLVTIHVALSQVPALITPAAELRAIRLADRACRQMGVGLGDKPPRIAVAGLNPH
                     AGEDGKFGREDIDAIAPAIAQAQAEGLDASGPWPGDTVFMRARRGEFDIVVAQYHDQG
                     LIPVKYLGVDHGVNVTVGLPFIRTSVDHGTAFDIAWQGKADQASLVEAFNLAIKMA"
     gene            29348..30340
                     /locus_tag="VGC69_03310"
     CDS             29348..30340
                     /locus_tag="VGC69_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY0294347.1"
                     /translation="MQRLARFLPERFTCLILLTVLIASLLPAHGQGIKIVGNIANLAV
                     ALLFFLHGARLSREAIVAGFTHWRLHLTIFSSTFLMFPILGLLLKPVLEPLVTPELYM
                     GILFLCCLPSTVQSSIAFTSMARGNVPAAVCSASGSSLLGIFLTPLLVGVVLAGGSSA
                     PMSFGAVETIMLQLLLPFILGQVLRRWIGGWVHRRKAMLKWVDQGSILLVVYTAFSEA
                     VSEGLWHNTPILSLVGLVVCNLIILALALLGSNTMGKVFGFSLPDRITLLFCGSKKSL
                     ATGVPMAQVIFAGQAVGAIVLPLMIFHQVQLMVCAALASRYAKRPEEPGEHAHH"
     gene            complement(30383..31756)
                     /locus_tag="VGC69_03315"
     CDS             complement(30383..31756)
                     /locus_tag="VGC69_03315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY0294348.1"
                     /translation="MNTPADSQLEYLRVPPHSIEAEQSVLGGLLLDNAAWERVSDTLR
                     EEDFYRHDHRLIWHHIARLIELSRPADVITVHESLTSSGKAEEVGGLAYINALAHNTP
                     SAANIRRYAEIVNERAMLRRLVSIADEISSAALNPQGKEAKQLLDEAESKVLQIAQEG
                     QRGLAGFQQIQPLLSQVVERIDELYHREGDSDVTGVPTGFTDLDKMTSGLQAGDLVIV
                     AGRPSMGKTSFSMNIGEHVAIEQGLPVAVFSMEMGAVQLAMRMLGSVGMLDQHRMRTG
                     KLTNEDWPRVTHAVSQMQDALLYIDETPALSAMDVRSRARRLARECGQMGLIIIDYIQ
                     LMSGNSGNSENRATEISEISRSLKGLAKELNCPLIALSQLNRSLEQRPNKRPVMSDLR
                     ESGAIEQDADVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGTVRLTFQGSSTRFL
                     NFSAAGY"
     gene            complement(31900..32355)
                     /gene="rplI"
                     /locus_tag="VGC69_03320"
     CDS             complement(31900..32355)
                     /gene="rplI"
                     /locus_tag="VGC69_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY0294349.1"
                     /translation="MQVILLEKVVNLGNLGEVVRVRDGYARNFLIPQKMARRATDAAL
                     KEFEARRVELEKTQAEKLSTAQALGERLAGYQLKVVQKAGVDGRLFGSVTNADIAEGL
                     HKAGFEGVAKAQVRMPTGQLKTVGESSVQVALHPDVLVEVSVLVEGDMA"
     gene            complement(32370..32642)
                     /gene="rpsR"
                     /locus_tag="VGC69_03325"
     CDS             complement(32370..32642)
                     /gene="rpsR"
                     /locus_tag="VGC69_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY0294350.1"
                     /translation="MMAFGKKKEKRKFTQQNPLFKRRKFCRFTAAGVEEIDYKDLDTL
                     RDFVQENGKIIPARLTGTRAIYQRQLDTAIKRARFLALLPYTDNHN"
     gene            complement(32704..33027)
                     /gene="priB"
                     /locus_tag="VGC69_03330"
     CDS             complement(32704..33027)
                     /gene="priB"
                     /locus_tag="VGC69_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEY0294351.1"
                     /translation="MNKVELVARVIEREPLRHTPAGLPAIEMLLEHESEVIEAGQPRR
                     VAFTVNAVALGDLAMLLSDIPLGAELRVEGFLAPVRKDSAKLKLHMQRAGRISGSAGS
                     DPMVA"
     gene            complement(33081..33461)
                     /gene="rpsF"
                     /locus_tag="VGC69_03335"
     CDS             complement(33081..33461)
                     /gene="rpsF"
                     /locus_tag="VGC69_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY0294352.1"
                     /translation="MRHYEVVFIVHPDQSEQVPAMVERYQALVTGQGGAVHRFEDWGR
                     RQLAYPIQKLVKAHYVCLNIEAGQATMDELEHSFRYNDAVLRHLVIKTKKAVTAPSIM
                     MKSVEREEARKAAAETAPAAAVTE"
     gene            complement(33772..34569)
                     /gene="folE2"
                     /locus_tag="VGC69_03340"
     CDS             complement(33772..34569)
                     /gene="folE2"
                     /locus_tag="VGC69_03340"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY0294353.1"
                     /translation="MNSPIDPAIVMPDVQSTADTRHIPIQRVGIRGVRHPMLVKAADG
                     SAQPTVANWTLTVALPAEEKGTHMSRFVALLEKHRATAMTPALFAEMAQEMLSLLHAE
                     RGDITAAFPYFINKSAPVSGVQSLLDYEVQWVARAAETGVEFELVVQVPVTSLCPCSK
                     AISEYGAHNQRSHVTVSAVLDGAVSMDEIIREIESQGSCELWGLLKRPDEKFVTERAY
                     DNPKFVEDLVRDVAARMQAHAGVRRFRVEAENFESIHNHSAYAVVEG"
     gene            complement(34700..>35870)
                     /gene="dxs"
                     /locus_tag="VGC69_03345"
     CDS             complement(34700..>35870)
                     /gene="dxs"
                     /locus_tag="VGC69_03345"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY0294354.1"
                     /translation="GMVSPATLFEEFGFHYVGPIDGHDLDALIPTLQNLKELQGLQFL
                     HVVTKKGQGYKLAEADPVLYHGPGKFDPRVGIQKSKAPAKQTFTQVFGQWLCDMAEAD
                     TRLVGITPAMREGSGLVEFEQRFPQRYFDVGIAEQHAVTFAAGLACEAQKPVVAIYST
                     FLQRGYDQLIHDVALQNLDVTFALDRAGLVGADGATHAGSYDIAFLRCVPNMVVATPS
                     DENEARLLLTTCYRHAGPASVRYPRGAGAGAPVTAGFDDVPLGKGVIRREGARIAILG
                     FGTLVPAALKAAESLGATVADMRFVKPIDRELVLDLAARHDALVTLEEAAIMGGAGSA
                     VAEALSQAGVAKPLLQLGLPDLFIDHGDQAALLTGLGLDAAGIEASIRARFSAFL"
BASE COUNT         6079 a        12236 c        11482 g         6073 t
ORIGIN      
        1 ccgcagcacg gtcctcttcg tcacccacag catcaccgaa gcggtgctgc tctccgacac
       61 ggtcgtcgtc atgagcgccc ggccgggccg catccgcgac gtcgtgcccg tcgacctgcc
      121 gcggccgcgc gcgcccgaca cgctgcgcga tccgcgtttt ctcgcgctct ccgagcgggt
      181 gcgcgaaggc atcgaagcgc aatgggtgga gtgacgacat gacccggcca atcccgaaac
      241 ggatggcggc gatcgccaag cccttgatcg cgttcgccct gatgctgctc gcctgggaga
      301 tcggagcgcc ccgggtgggc attcccacct acgtgctgcc cctgcccagc atgatcgcgc
      361 agcgctttgc cgaaacgttc tcggtgcaga tgctgggcct ggccatgacg gccttcacca
      421 ccctggccgg cctcgggctc gcgctggtgt tcggcgtgct gctggcgctg gccatcatct
      481 atgtgccgct gctgcgctcg atcgtcacgc cgctgctcgc ggccttcaac tccatcccga
      541 agatcgccat cgcgccgctc ttcgtgatct ggttcggcct gggctatgaa tcgaagatat
      601 tgctcgcctt cctcctggcg ctgttcccga tcttcgtgaa ctcggtaacc gggctgggcg
      661 agatcgaggc cgacctcatc gacctctcga cgctcgccgg cggcacgacg tggcgcatat
      721 tcatgaaggt gcggctgatg aacgccgtgc cctacatcgc cgacgcggtc aaagtggcct
      781 ttcccctggc gctggtcggc tccatcgtcg gcgaattcat cggcggcaac agcggcatcg
      841 gccacctgat cctgtcgggc cagttcaatc tcgatacgcc gctcgttttc gcgacgctct
      901 tgtccatcac cctgttcacg acgctgggca tcggcgccgt gtccctgttc gaacatgcct
      961 tcctgacctg gcgcccctcg cagcgccagc gctgatgccg gcgcaccacc ccatcgcctt
     1021 cggagtccaa cccatgcgtg actacctctc ccgcctgcgc caacgcggcg acctgctcgt
     1081 cgtcgacaaa gagatcgacc ctttccacga actggccgcc gtcacgcatc acgcccagaa
     1141 aaaatgggcc aagccgattc tcttcaccaa cgtcaagggc acgacattcc ccgtcgtcac
     1201 caatgtctac ggcacgcgcg agcggctggc ggaagtcatc ggcatcgacg ccaacgattt
     1261 ctgccgccaa tggagcaacc tggcctcgct gggcgccgcg gccatgccag aaccgttggt
     1321 gcccgcgcgc gccgcgcccg actatgtcga cgtcaagctc tccgacctgc cgctcatcac
     1381 ctattccgac cgcgatggcg ggccctactt cacctcggcc atgttcatcg ccaaggaccc
     1441 ggaaaccggc gtcggcaatc tctcctacca ccggtcgatg tacgtcagcg acaacgagct
     1501 gcgctgccgc ctggcgccgc gccaccacct gacgatctac cacgaaaagg ccgagaaaat
     1561 gggcaagccg ctggaagcgg ccatgctgat cggcgcgccg tcgcaggctt tcctgaccgc
     1621 cgccgccccc ctgccctacg acgtggacga actcgaagtc gccgcgcggc tgcgcggctc
     1681 gcccatcgag atgcgcccat gcaagcacat cgacctcgaa gtccccgcag aaaccgaaat
     1741 cgtgatcgaa ggccgcttcc tgcccaacga gcgcaggccc gaagggcctt tcggcgaatt
     1801 catgggctac tacgtcccgg tcggcccgaa cgccgttttc gaagtcctgg gcgtcaccgc
     1861 gcgcaaggac gccatgttcc actcgatact ctgcggctcg cccgaagaag tgctgacgct
     1921 ggaactctcg gtatccgcca atatctacca acgcctgagc gccgcgctgc ccggcatcgt
     1981 caacgtgacc tgccagccct tcgtcaatca cgctatcatt cagatagacc cgcaattcga
     2041 gggccacgcg cggcaagtga tgctggccac catcggcgcg gagcccatct gggccaagat
     2101 cattacggtg gtcgaccgcg atgtggacat ctacgacatg gacgacgtca tgtgggccat
     2161 cctgacccgt tcgcgccccg acaaggacac gctcatcatc cccgacacgc cctcgttcta
     2221 ccgcgacgaa ttcaaggacc actggggccg gctgctgatc gatgccacca agccctgggg
     2281 ccgcgaaaag gaattcgagc gcaagcgcct gcgcagggtc aacgacgtgc gcctgtccga
     2341 ttggttcaag gaggcctgat ggcccaggcg caacggctcg tcatcggcat cacgggcgct
     2401 tcgggcgccg tctatggcgt gcgcgcgctc gaattggcgc gcggcgccgg cgtcgaaacg
     2461 cacctgatcg tcagccgctc cgcgctgctc acgctgaacc aggagctcgg cctgcagaaa
     2521 gaagacctgg ccgcgcgggc cgatgtgatg catcccgtcc aggacatcgg cgccaccatc
     2581 tccagcggct cgttcaagac catgggcatg ctgatcgcgc cctgctcgat acgcacgatg
     2641 tcggaaatcg ccagcggcgt cacgtccacc ctgatcagcc gcgccgccga tgtggtcctc
     2701 aaggaacgcc gcaagctcgt gctcatggtg cgggaaacgc ccctgcacct gggacacctg
     2761 cgcagcatga cccagcttac cgaaatgggg gcgatcatca tgccgcccgt gccggccttc
     2821 tatgccaagc cggacacgct cgacgacatg gtcaaccata cggtggcccg cgcattggac
     2881 ctgttcggca tcgagacgga tgccgtcaag cgctggggcg gcctcaaggc cgaataagcg
     2941 caggcccagg cgggggccaa acaggccgtc aggccgtgcc gcccaccgtc aggccgtcca
     3001 tgcgcaccgt gggcatgccc acaccgaccg gcacgctctg cccttccttg ccgcacgtgc
     3061 ccacgcctga atccagcctt aaatcgttgc cgatcaggct cacgcggttc atggcgtcgg
     3121 ggccgttgcc gatcagggtt gcgcccttga ccggataggt gaccttgccg ttctcgatca
     3181 tataggcctc ggaggccgag aacacgaact tgccgctggt gatgtccacc tggccgccgc
     3241 cgaagttcac ggcgtacagg ccgcgcttga ccgaagccac gatttcctcg gggggcgtgg
     3301 tgccggccag catataggtg ttggtcatgc gcggcatggg caggtgcgcg aaggactcgc
     3361 ggcgtccgtt gccggtggcg cccgtcttca tcaggcgcgc gttgagcgtg tcctgcatat
     3421 agccgcgcag gatgccgtcc tcgatcagca cgttgtgctg ggtggcgttg ccctcgtcgt
     3481 ccacattgag cgaaccgcgg cggcccggca aggtgccgtc gtcgatcacg gtcacgccct
     3541 tggaggccac gcgctggccg atgcggccgc agaacacgct ggagcccttg cggttgaagt
     3601 cgccctcgag gccgtggccc acggcctcgt gcagcaggat gccgggccag cccgagccca
     3661 gcaccaccgt catctcgccg gccggcgcgg ggcgggcctc gagattgacc atggcctcgt
     3721 gcacggcgcg gtccacgtat tcctgcatca cttcgtcggt gaacatttcc aggccggtgc
     3781 gcccgccgcc gccgccgtgg cccatctcgc gccggccgtt gcgctcggcg atcacggtga
     3841 tcgacaggcg caccagcggc cgcacgtccg cggccaggcg gccgtcgctg ccggccacca
     3901 gcaccacgtc gtattcggcg cccaggccgg ccatgacctg gatcacatgc gggtcggccg
     3961 cgcgcgccat gcgctcgatg cgctccagca ggacgacctt ctcggccgcg ctcagcgtgg
     4021 ccagggggtc gatgtcggta tacaggctgc gggcatcgtc aggcggaacc tgggccgata
     4081 ccttgagctt gcccgcgccg cggcgcgcaa tgccgcgcac ggtatgggcc gcggacatca
     4141 gcgcctcggg ggacaggctg tcggaatagg cgaaggcggt tttctcgccg ctgaccgcgc
     4201 gcacgcccac gccctgcccg atcgagaaac taccggtctt gacgatgccc tcttccaggc
     4261 tccagccctc gctgcgggtg tattggaaat acaggtcggc ataatcgacc ttgtgcgtga
     4321 atatttcgcc cagggcatgg gccaggctgg cctcggtcag gccccagggg tcgagcagca
     4381 gggatttggc ggtggccagg gattgaatcg cgggatcgat cagtttcatt cgtttggctt
     4441 gggcggattt tctgcacgga ttcgcgtgca ggctgtacgg acggttttta tagcgggttt
     4501 tttacaacac gcgatggcgc agggccggca aggccgcgcg cacatcggac aggcgttcag
     4561 gctctatggt accgccgatc acgcccggcc cttcgggcag cacgtcgata atctgtcccc
     4621 aggggtcgat cagcatggag tgcccccagg tgcgccggcc attggggtgc gtgccgccct
     4681 gcgccggcgc cagcacatag cactggttct cgatggcgcg ggcgcgcagc agcgtctccc
     4741 aatgcgcctg tccggtggta taggtaaatg ccgaaggcac caggatgagg ttgaccggac
     4801 ccatattgcg gtacagctcg gcaaaacgca ggtcgtagca aatggaaagc cccaccgggc
     4861 cgcagggcgc ctcgaatatc tgaatatccc cgcccggctg tatggcgacg gattcgtcat
     4921 aggcctcggc gccgcgcttg aaactgaata aatggatttt gtcgtagcgg gccttgcatt
     4981 ttccgtcggg gccgaacacc agggtggtgt tgtagacttt attagggtcg gggcaatcca
     5041 gcggcatgga gccgcccgcc agccagatgc cgtgacgttc ggccgtgcgc gccaggaaat
     5101 cctggatggg accgctgccg aaagtctcct tgacggccag tttgtccgtg tcgcgccgcc
     5161 ccagcaggca gaaatactcg ggcagggcca ccagtttggc gcccgagccg gcggcttcgg
     5221 caatcagccg gccggcggcc tcgagatttt cggaaacctc gggcgtgctg accatttgca
     5281 cggcggcaat ttgaaaaggt gaaaaaatct ctgtcatttt cctttcgccc gggatataaa
     5341 tgtcatataa aaaatattct cgccggaaaa ataaatagta ttattccgcc accgaggcca
     5401 tttaagcttt aaccaagtac tgccggtact ggtttaaaaa acgggtttgc aagcaagcat
     5461 gcgggcagac gcgcaagcac cgaatggcct acagaccaac cgaggaatac atggcccgag
     5521 tcacctacgc cagccagcag gcaaagatcg aaaaagaaat caccaagctg caaaagcagg
     5581 ctgccgcgct gcaaagcaag cgccgcaaac ccatcattgc atccatcgtc aaatcgatgc
     5641 gtgagtacga tattactccc gaagacatcg tagcgaccta caactcgacc aaacccggcc
     5701 gcggcgccaa gcgcaaggtt gccagcggct cggccagcac tgccagcgcg gccgccaagc
     5761 gcgccgtcgc gcccaagtac cgccaccccg agaccgggga aacctggagc ggccgcggca
     5821 aggccccccg ctggctgatg gcggccgaag ccgacggcgc atcgcgcgac agcttcctga
     5881 tcgcctgata cgtctgtcgt atcgactttt caaattacac ggttcagtat gaaccgtgct
     5941 cgtggcgcgc cctaggcgcg ccattttatt tcccctctcc gaatagggta ttaactctgc
     6001 ccggaccatt cttcaatcgc cggatatgca gcaaaaataa tacgaaattc actattttgg
     6061 gtttatccga attaatttat ttcatggcgt ttcattcaat tgattcatcg gatcggcaat
     6121 aacgcgcctg cctgggccgc cgtccggccg ccatcggggg cccgcgccgc ggacgggcta
     6181 ctacaatagc catcattccc agccgcaaag agacgaagca tcatgcgtac cgacatgccc
     6241 gtgaccatct atcgcaagga ttatcggccc tatccgtacg cgattcccca ggtagccctg
     6301 gccttcgatc tcgaccccga cgtcaccacg gtgcactgcg ccatgcaggt cgagcgccgc
     6361 ccggatgccc cggccgacgc ccccctggag ttcgacggcg aggcgctgga gctggtctcg
     6421 ctgcgggtcg acggccagcc ctggcccgcg gcgcagtaca cggtcgacga gcacagcctg
     6481 gtcatccgcg gcctgcccgc gctcgcccgg gtcgaaacca tcagccgctg ccgcccggcg
     6541 gccaactcca cgctgatggg cctgtacgtc tccggcgaca acttcttcac ccagtgcgag
     6601 gccgagggtt ttcgccgcat tacctggttt gccgaccgtc ccgacatcat gtcgcgctac
     6661 cgcgtcacgc tgcgcgcccg ccccgactac cccgtgctgc tgtccaacgg caacctgatc
     6721 gccgagcgcg aactgcccga cggccgccgc gaggccgaat gggaagaccc cttccccaag
     6781 ccctgctacc tgttcgccct ggtggccggc aaactcaccc accgcgaaac ccgcatcaag
     6841 accatgtcgg gccgcgatgt gctgttgcag gtctacagcg accccggctc ggaatcgcgt
     6901 acccagtggg cgctggattc gctggtgcgc gcggtgcatt gggacgaaaa gcgtttcggc
     6961 ctggagctcg acctggaccg cttcatggtg gtcgccgtgc gcgatttcaa tatgggcgcc
     7021 atggagaaca aaggcctgaa catcttcaac gcagcctatg tgctggccga ccccgacacc
     7081 gccaccgacg ccaactacga gggcatcgaa tcggtcatcg gccacgagta cttccacaac
     7141 tggacgggca accgcgtcac ctgccgcgac tggtttcagc tcagcctgaa ggaaggcctc
     7201 accgttttcc gcgaccagga gttcagcgcc gacatgatgg cgcgcgagat actgtcagcc
     7261 cccacgctgc gccctgcggg cttgctgccc cccgaggggg cccgcccgcc ttggggcggc
     7321 ccggcggcgg gctgcgcggc agccgccgcc agcgcccgca ccgtcaagcg catcgacgac
     7381 gtggtcaccc tgcgcgctgc ccagttcccg gaagacgcgg gccccatggc ccatcccatc
     7441 cgccccgaca gctaccagga aatcggcaac ttctacaccg ccacggtgta tgaaaaaggc
     7501 gccgaggtca tccgcatgca gcacaccctg ctgggcgagg cgggctttcg cgccggcatg
     7561 gacgaatact tccgccgcca cgacggccag gccgtgacct gcgacgactt cgtcgccgcc
     7621 atggagtcgg tctatgtcag gcagcacccc ggccgcgacc tgcagcagtt ccgcaactgg
     7681 taccgccagg ccggcacgcc gcgcgtcagc gtggcgctgg gctatgaccc cgccgcgcgc
     7741 cgctgcaccc tcacgctgtc gcagagctgc ccgcccgtgg gcgtcgagcg ccgccccggc
     7801 cagccggcca agcagcccct gcacattccc tttgccatcg gcctgctcga tcgcaccggc
     7861 aagcccatgc cgctcgcgct ggacgatcag ccccccaccg gcaccgtgct gctggaactg
     7921 accggcgaac gccagcaatg ggtgttcgac ggcatcccct cggcgcccgt gccctcgctc
     7981 ttgcgcggct tctcggcgcc ggtcatcgtc gactacgcct ggacggacga agagctggcc
     8041 ctgctttcgg cccacgacac cgatgccttc gcccgctggg aagccggcca ggaactcgcc
     8101 acgcggcaga tcctggcgct cacgcgccag caccaggccg gccagcccat ggcggtggcc
     8161 gacgccttca tcgaagcctg gcgcgccctg ctcaccgacc cggcgctgga tgccgcctac
     8221 cgggcccgca ccctgctgct gccctcggaa aagaccctgg ccgaacgcat ggaaggatcg
     8281 gtcccccaag ggggcgattc cgtcttgggg cggcccggcg acggatcggg cccccacgcc
     8341 gcgcccttcg ggcttgctgc cccccagggg ggcgattccg tcttgggacg gcccggcgac
     8401 ggaatggacc cgccggccct ggccgccgcg cgcgaccatc tgcgcgccga gctcggccgg
     8461 cggctcgccg ccgaatggcg cgccgccttc gacgccaacc agacgcccgg cgcctacagc
     8521 ccggcgcccg gcccggccgg catgcgcgcc ctgaagaacc aggcgctggc ccacctgatg
     8581 gccggcggca tcgcggacgc ccaggccctg gccgagcggc aatacgccac ggccggcaac
     8641 atgaccgaca gcatggccgc gctctccagc ctgatcaact atggcgcggg cgacgccccc
     8701 gcccgcgccc tggccgcctt ctacgagcgc tggcaaggcg acccgctggt cgtcgacaaa
     8761 tggttcacgc tgcaggccac cgcgcgcgcc accgacgtgg cggccgtccg cgcgctcatg
     8821 cagcaccccg ccttcaccct gcgcaatccc aaccgggccc gcgcgctggt gttccagttc
     8881 tgcctgaaca atgcccgcgg catgcaccgc cccgacggcc tgggctatgc ctactgggcc
     8941 gaccaggtca tcgcgctcga cggcctgaat cccgaggtgg ccgcccgcct ggctcgcggg
     9001 ctcgacagct ggtcgcgctt cgtgccgccg ctgcgcgatc ccatgcgcca ggcgctggag
     9061 cgcgtcgccg gccaccccgg cctgtcgcgc aacgtgctgg aaatcgtctc gaaagctttg
     9121 gaattcgccg cacaggcata ggagatcccc cgtgaagaac aagaccctca cccaatacct
     9181 ggtcgagcag cagcgctcgg cccagctgct gcagcccgag gtgcggctgc tgatcgaagt
     9241 ggtggcgcgc gcctgcaaga gcatcggcca cgcggtcggc aagggcgcgc tgggcggcgt
     9301 gctgggcgcg ctggagaccg aaaacgtcca gggcgaggtg cagaagaagc tggacgtgct
     9361 gtccaacgaa atcctgctcg aggccaacga atggggcggc cacctggcgg ccatggcgtc
     9421 cgaggaaatg gaaaccatcc accccattcc caaccgctac cccaagggcg aatacctgct
     9481 gctgttcgac cccctggacg gctcgtcgaa catcgacgtg aacgtctcga tcggcacgat
     9541 cttctcggtg ctcaaggcgc cgcacgccgt gggcgaggca gtcaccgagc aggacttcct
     9601 gcagcccggc gcgcgccagg tcgccgccgg ctatgcggtc tacggccccc agaccatgct
     9661 ggtgctgacc gtcggcaacg gcacggtggg cttcacgctc gaccgcgaaa tgggctcgtg
     9721 ggtgatgacg caggaaaagt acgtcatccc cgaagacacc aaggagttcg ccatcaatat
     9781 gtcgaacatg cgccactggg ccccgcccat gaagcgctac atcgacgact gcctggccgg
     9841 caagacgggc cccctgggca aggactacaa catgcgctgg atcgcctcga tggtggccga
     9901 cgtgcatcgc gtgctcaccc gcggcggcat cttcatgtac ccctgggata cgcgcgagcc
     9961 ttccaagccc ggcaagctgc gcctgatgta cgaagccaac cccatgagcc tcctggtcga
    10021 gcaggccgga ggcaaagcca tcaccggcac gcagcgcatc ctggaggtgc agcccgagaa
    10081 actgcaccag cgcgtgagcg tgatcctggg ctcgaagaac gaggtcgaac gcgtcggccg
    10141 ctaccacgcc gaagacgcgg gcaagtaagc gatcccatgg caaggtaaaa ccggcctggc
    10201 gaaaagccgg acaagaaaac tggacatcat ccttgtcgat gcggatgacg cggccgctat
    10261 ccttgtactc gtagacaccg ccgagttgaa ggtcggccgg tctggcgtca cgaccgcggc
    10321 atcgcgtacg gaagtcttac atttttgcgt gggattctat gtagtgccgc accgactgta
    10381 cgttgcaatc catcacttcc acgcgctgcg gcagcgattc cagccccgcc agcgtggccg
    10441 gcacgggcgc cgggcggccg atggcctgct ggatggtttc cgagaacttg gccggcagcg
    10501 cggtctcgag caccagcatg ggaatgccgg gctccacgta ctgggccgcc actttcacgc
    10561 cgtcggcggt atgcgggtcg atcagcacgc cggtggattc cagggtggcg cggatcgcgg
    10621 ccaggcggtc ggcatggctg ctctgccccg ccacgaagcc gaactcggac tcgaagcgcg
    10681 ccttttccga cgacaggtcg aagcgcccgg tctcggccag ttcggcccac aaggccttga
    10741 cccgcgcgcc gtcgcggccc accaggtcgt ggacgaagcg ctcgaagttc gaggcgcgtg
    10801 aaatgtccat ggacgggctg gacgtggcgt aggtgtgctc ggggttgcgc ggggcgtaga
    10861 tgcccgtgcg gaagaactct tccagcacat tgttctcgtt ggtggccagc accaggcggc
    10921 gcaccggcag gcccatgccg cgcgcgatgt ggcccgacag aatgttgccg aagttgcccg
    10981 agggcaccgt gaacgacacc tgctggcccg cgcccgtggt ggcgcgcagc cagccccaga
    11041 agtagtacac cacctgggcc gaaatacgcg cccagttgat ggaattcacc gcgcccaaat
    11101 gcacgcggcg cttgaaatcc aggtcgccgg ccagggcctt gacgatgtcc tgcgcctcgt
    11161 cgaacacgcc gcgcacggcg atgttgtgga tgttctcgtc ctgcagcgaa tacatctgcg
    11221 cgcgctggaa agcgctcatg cggccgtgcg gcgaaagcat gaacacggcc acgccctgct
    11281 tgccgcgcag ggcgtattcg gcggccgagc cggtatcgcc cgaggtggcg cccacgatgt
    11341 tcaaggtggc gccgcgcttg gccaggacgt attcgaagat ctggcccagg aactgcatgg
    11401 ccatgtcctt gaaggccagc gtggggcctt cggacaagcc cagcagggcc atcgaggtat
    11461 tcaagggctt gaccgggacg atctcggcgc tcttgaaggt ggccggccgg taggccgccc
    11521 gcaccagctt gcgcaggtca tcggccggca cgtcggtgac aaagcgcgag agcacctcga
    11581 aggccaggtc ggcataggcc aggccgcgcc aggactccag catggcggcg tcgaccctgg
    11641 gcaggtcctg cggaaccgcc aggccgccgt cgggcgccag gccctcgagc aggatgtcgg
    11701 aaaacgattg cggggccatg ccgccgcgcg tggaaacgta attcgtcatg ccagcctcgc
    11761 ctgtcggcgg acaaagtggg cgcgatggat cattgcaggt tctcgatgcg caggcgcgtc
    11821 accttggagc gcacgaaagg cagcacctcg atcgcggcga tcgcctggtt gaccttgcct
    11881 tccaccgcct catgcgtgag gaagatgatg tcggcgccgc ccagattcga cggctgctgg
    11941 atcatcgaac cgatggagat gccgcggtcg gccaggatgc gggcgatgtc ggccagcacg
    12001 ccgggcacgt cgtcgacccg caggcgcagg tagtacgagg tctgcacgcg gtccatgggc
    12061 aggatggcca ggtcggacat cgaatcgggc tggaaggcca ggtgcggcac gcggttgccc
    12121 gggtcggccg tatgcaggcg ggtgatgtcg accaggtcgg ccaccacggc cgaggcggtg
    12181 ggctcctcgc ccgcgccctg cccgtagtac agcgtggggc ccacggcgtc gccgcgcacc
    12241 agcacggcgt tcatggcgcc ctcgacattg gccagcagct tgtcggccgg caccagcgcg
    12301 gggtgcacgc gcagctcgat gccgtcggcg cggcggcgcg tgatgcccag cagcttgata
    12361 cggtagccca gccgttcggc atgcgcgatg tcggcggact ccagccgcga gatgccctcg
    12421 atgtgcgcct tgtcgaactg caccggcacg ccgaaggcga tcgaggccag cagcgtcagc
    12481 ttgtgcgccg catccacgcc ttccacgtcg aaggtcgggt ccgcctcggc atagcccagg
    12541 cgctgcgcct cggccagcac cgtgtcgaag ggcagcccgc gcgagcgcat ctcggacagg
    12601 atgaagttgg tcgtgccgtt gatgatgccc gccagccact cgatgcggtt ggccgtcagg
    12661 ccttcgcgta tggccttgat gatgggaatg ccgccggcca cggccgcctc gaaggccacc
    12721 atcacgccgc gctcggaagc cgcggcgaag atctcgttgc cgtgcttggc cagcagcgcc
    12781 ttgttggccg tgaccacgtg cttgccgtgg gcgatggcct cgagcaccag ctcgcgggcc
    12841 agcgtgtcgc cgccgatcag ctcgaccacg atgtcgatcc cggggtcgcg caccaggccg
    12901 tgcacgtcgg tggacaccgg cacggcctcg cccaccttgc tgcgagcctt ggccacgtcg
    12961 cgcacggcga tgcgcgccac ttcgatgcgc cggccggcgc ggcgggcgat ttcctcggca
    13021 ttgcgcgcca gcaccgtcca ggtgccgccg cccaccacgc ccaggcccac gagcccgacc
    13081 ttgatcgggc ccatcgcctc ggcctggcca accttgaggg ggctcgtcat ttcagcaaac
    13141 cgtccttgcg gaacatatcc ttgatgccgc gcacggcctg gcgggtgcgc tgctcgtttt
    13201 cgatgagcgc gaagcgcacg tattcatcgc cgtattcgcc gaagccgatg ccgggcgaca
    13261 ccgccaccct ggcctcggcc agcacgtgct tggcgaattc cagcgaaccc atggcgcgat
    13321 agggctcggg gatgtgcgcc cagatgtaca tggaggccct gggaatgtcg acgttccagc
    13381 cggcctcgat cagcccgcgg gccagcacgt cgcggcggct ctggtattgc gccacgatct
    13441 cgtcgacgca gtcctgcggg ccgtccagcg cggcgatcgc cgccacctgg atgggcgtga
    13501 aggtgccgta gtcgtggtag ctcttgatgc gcgccagcgc gttgacgaga tccttgttgc
    13561 ccaccatgta gccgatgcgc cagccggcca tgttgtagct cttgctcatg gtgaagaact
    13621 cgacggccac gtcgcgcgcg cccggcacct gcatgatgga cggcgccacg tagccgtcga
    13681 agcagatgtc ggcgtaggcc agatcgtgca ccaccaggat gtcgtgctcc ttcgccagcg
    13741 ccaccacgcg ctcgaagaag gacaggtcca cgcactgcgc cgtggggttg ctcggaaagc
    13801 ccaggaccat catcttgggc ttggggatgg actcgcgcac cgcgcgctcg agctcttcga
    13861 aaaagtccac gcccggtgtc atgcgcaccg agcggatgtt cgcgcccgcg atcaccgcgc
    13921 cgtagatgtg gatgggatag ctggggttgg gcaccagcac cgtgtcgccg cggtccagcg
    13981 tggccagcat caagtgggcc aggccctctt tcgagccgat ggtgacgatg gcctcggaat
    14041 ccgggtcgaa ctccaccgcg tagcggcgct ggtaccagtc gcagatggcc ttgcgcaggc
    14101 gcgggatgcc cttggacacc gagtagccgt gcgtggtggg ccgcagcgtg gcctcaacca
    14161 tcttttcgac gatatgccgg ggcgtggggc cgtcggggtt gcccatggac atatcgataa
    14221 tgtcttcgcc gcgccgccgc gccgccatct tgagctcggc ggtaatgttg aaaacataag
    14281 gcggcaggcg ctcgatgcgg gtgaacgttc gcgcgggttt gtcggagcca ctcacgatgg
    14341 acacatcctt acactaaaaa atacgctgga aaaccctgta atctagcagc aagcccgccc
    14401 tggcggcaaa tgggcctcat ccgtcccccc gcctgtgtgc gcaacgcaac atacagacat
    14461 ggcgatgcgc tattatcggg acgtccagcc tgcgttttca ttgaagctga gcaatattga
    14521 aactgcataa cgatcccgcg acgacgctca acacggtcac cgcttacggc cccgactata
    14581 tcgaggtcaa ccaggtacgc ttttcccacg cggtagcctt cggtcccgaa ggcgccgtgg
    14641 cgcaatggcc ggtcgagtcg ccggccgaca ttacctccgc cctgctccaa caagccgccg
    14701 gcctgtccca ggcatcgcgc gaccccctgg cttttctcga cgaccccgag tccgccgccg
    14761 gcggcatggc cggcgttcgt ccgcccgatg cccccgaagt gctgctggtg ggcaccggcg
    14821 cgcgccagaa aatgctgccg cccgccgtcc tgcgccccat cctggccgtc ggcgtcggga
    14881 tcgaggtcat ggatacccag gccgcggccc gcacctacaa catcctcatg gccgagggcc
    14941 ggcgcgtcat cgtcgcgctg ttgcccacca acgtgcccac ccatggagaa tccgcttgat
    15001 gtccgcgctc atcggcaagc ccgccccgca gttcgtcgcc gaaagcacca tcggcaccat
    15061 tgatctgtcg caatgccgcg gccgcgccgt ggtgctgtac ttctacccca aggacaacac
    15121 gcccggctgc accaccgagg gccaggactt ccgcgacctg cacgaccaat tcctcgccgc
    15181 cagcacagtg gtcctcggcg tgtcgcgcga cacgctgaaa tcgcacgaga atttctgcga
    15241 aaagcacaac ctgccctttc ccctgatcgc cgatcccgac gaaaccctct gcaatctgta
    15301 cggggtcatg aaacagaaga atttgtacgg taaacaagtg cgcggcatcg agcgcagcac
    15361 cttcctgatc gacgcctacg gcctgctggt gcaggcttgg cgcggcgtga aggtgccggg
    15421 ccatgccaag gaagtcctgc aggccgccag cagcataggc tgaccggctg cgcgacccgc
    15481 gccgattcgt acccgcgttc gtacccgggc caatcccggc ccacacagga gagtcaaccg
    15541 tatgccgctt ccgaagttgc ccacccgtcc tgctgccatc ctgactttcc ccatgggcga
    15601 gaccgcgccc gccgcaagcc ccgctccccg tcccgccccg cccgccgccc tcgaacccca
    15661 tacccaccag ccccagctcg acggcatgga agccgcggtg gttccgatcg ccgatgccgg
    15721 cgcatcggcc gcgcccatcg ccgcccgctc gcccgagccg caagccaagc cggcgcccgc
    15781 cgccgcccgc aagtccaagg ccaagacgca gccggccgtg cgcaagctct tcgtgctcga
    15841 caccaatgtg ctgctgcacg accccagctc gctgttccgc ttcgaagagc acgacatctt
    15901 cctgccgatg atgacgctgg aagagctcga ccaccagaag aagggcatgt cggaagtggc
    15961 gcgcaacgcc cgccaggtga gccgctcgct cgactcgctg gtgcaaaatg cgaccgacct
    16021 cgacctgggc gtggacctca ccacgctggg caacaaggac gccaccggcc gcctgctgtt
    16081 ccagaccacg gccatccaca gcaccctgcc ctcggacctg cccatgggca aggccgacaa
    16141 ccagatcctg ggcgtggtgc gcgccctgca ggaaaagtac acccagcgtg aagtcgtgct
    16201 ggtgtcgaaa gacatcaaca tgcgcctgaa ggcccgcgcg ctggggctgc aggccgagga
    16261 ctactacaac gaccacgtcc tggaagacac cgacctgatg tacgcgggca tgatgcagct
    16321 gcccgaggac ttctggaacc gccatggcaa ggatgtcgag tcctggcagc agggcggcac
    16381 cacgttctac cgcatcaagg gcccgctgtg cgcgcagttc atggtcaacc agttcgtgta
    16441 cttcgagggg cagatgccct tgtacgcgca ggtgcgcgag gtcagcggca agacggccgt
    16501 gctggccacg ctgcgcgact acacgcacgg caagaacaac gtctggggca tcacggcgcg
    16561 caaccgcgaa cagaacttcg cgctcaacct gctgatgaac cccgacatcg acttcatctc
    16621 gctgctgggc caggccggca ccggcaagac gctgctggcg ctggccgccg gcatcacgca
    16681 ggtgctggaa accaagcgct acaccgagat catcatgacc cgcgtcaccg tgccggtggg
    16741 cgaggacatc ggctttctgc ccggcaccga ggaagaaaag atgctgccct ggatgggcgc
    16801 gctcgaggac aacctcgacg tgctcaatat gggcgaaggc gacagcggcg gcgactgggg
    16861 ccgcgcggcc accatggacc tgatccgctc gcgcatcaag gtgaagtcgc tgaatttcat
    16921 gcgcggccgc acgttcttga acaaatacct catcatcgac gaagcgcaga acctcacgcc
    16981 caagcagatg aagacgctgg tgacgcgcgc gggccccggc accaaggtga tctgcctggg
    17041 caatatcgcg cagatcgaca cgccctacct gaccgaaggc agctcggggc tgaccttcgt
    17101 ggtcgaccgc ttcaagggct ggccgcatgc ggggcacatc acgctgcagc gcggcgagcg
    17161 ctcgcgcctg gcggattatg caggcgacgt gctgtagcct gcgcgtcccc tcctacaggc
    17221 gtaccacgat gcgcccgcgc acctcgccat ccatgaggcg ccgggcggca tcgacggctt
    17281 cgtccagcgc gatctccgtg gtgatggccg ccagcagcga cgggtccagg tccgcggcca
    17341 ggcgccgcca ggcctcttcg cgcagcgcga tcggggccat cacgctatcc acgcccagca
    17401 aggcgacgct gcgcagaatg aacggcatga cggtcgccgg gaaatccggg ccctgggcca
    17461 ggccgcaggc cgcgaccgcg ccgccgtagc gggtctgggc gcaggcattg gccagcgtgt
    17521 ggctgcccac cgcgtcgatg acggcggccc agcgttcttt ttgcagcggc ttgcccggcg
    17581 cgccgagttc ggcccggtcg atgatgcggg ccgcccccaa ggattgcagg tagccagact
    17641 cggcctgctt gcccgtggcc gcgacgacgc aatggccgag cttggccagc agcgccacgg
    17701 ccaccgagcc cacgccgccc gtggcgcccg tgaccaggat ttctccatcg ccggccttca
    17761 cgccatggcg ctccagggcc atgacgcaca gcatggcggt gtaacccgcc gtgccgatcg
    17821 ccatggcctg gcgggtagtg aatgcctgcg gcacgcgcac cagccaatcg cccttgaggc
    17881 gcgcacgctg cgccaggcag cccttgtgcg cttcgcccac gccaaaaccg ttgagcagga
    17941 cgcggtcgcc tgcctgccat gccgggtgcg aactggacat gacggtgccg gcgccatcga
    18001 cgcccgccac catgggccag ttgcgcacga cgggagcacg gttcgtgatg gccaggccat
    18061 ccttgtagtt caacgtggaa tactcaacgg ctatcgtgac gtcgccctcg ggcaggaagt
    18121 cgtcgcctac tgcgcggatc gccgcgtgga attccggctc tttatcgagg accagggctt
    18181 tgaacatggg tatattcctt gggcagggag tcaaacgaac gacgcatcac tccccatacg
    18241 ggtcgtagtc gaagtacttc ttggcgatcg tcatgtaggt gccgtccttg cggatggcgg
    18301 caatggcttc gttgatccgg gtccgcaggg catcgttgcc tttttccagg ccgatggcag
    18361 tgccgtcgcc gaccaggtcc ttgtcggtga tcttctcgcc caccatgcag aagcccttgc
    18421 cctggggctg atccagaaag ccgatttgcg ccggcgcggc ggactcgaac gacacgtcga
    18481 tgcggccggc gaccaggtcg gcgcgcacac cttccatatt ggggtatggc accagctcga
    18541 cgcccttggg cgcccagtgc gtcttggcat aggcttcttg gctgctgccc aggcccacgc
    18601 cgatgcgctt gccgcgcagc gactccggcg tggcgtggag ttcggttccg cacttggcca
    18661 ccagggcgcc tatgccggcg aacaggcgat cggtgaaatc gagctgttca cgccgcttgg
    18721 gcgtcgccgt catgccggaa agaatggcgt cgaacttctt ggccttgagc gcgggaatga
    18781 tgctgtcgaa gggttgctgc acccacatgc acttggcatt caggcgcttg cagatctcgt
    18841 tgccgagatc gacatcgaaa ccggccagcg acccgtcggc caggcgatat tcgtagggac
    18901 ggaaagtcgg gtcgacgccg aagcgcaggg tgtcgctttg ggcctggctc gccagcggca
    18961 atgccgcggc ggccagggtt gcgagcgtga agatgaactt gcgcatagat acccctctat
    19021 aactctgtca ggatgaaacg gcgaggccgt ggatcgctta cttcaactgc ccggccagaa
    19081 actgcgccag gcggacgctc ttgggccgtt ccagcacctc gcgggcatcg ccttcttcct
    19141 cgatcttgcc ctgatgcagg aacagcacat gcttggagac attgcgcgcg aaccccattt
    19201 cgtgggtcac gacgatcatg gtgcggccct cctcggccag gttctgcatg accttgagca
    19261 cctccccgac cagttccgga tcgagcgccg acgtgggctc gtcgaacagc atcacctcgg
    19321 gctccatggc cagggcccgg gcgatggcca cgcgctgctg ctggccgccc gaaagatggg
    19381 ccggatactg gctttccacc ttggcgctca ggccgacctt ggccaggtag tggcgtccgc
    19441 gctcctcggc gtcggcgcgg ctcaggccca gcacgtgtat cggcgcctgc gtgacgttct
    19501 gcagcgccgt catgtgcgcc cacaggttga agtgctggaa caccatcgac agcttggcgc
    19561 ggatcttctg caattgccgc gggtcggcca cgcgcaggcc ctgttcgccc ggcagtgtgc
    19621 gtatcagttc gtcgtcgacc catatctggc cggcgctggg cgtttccagg aaattgatgc
    19681 accgcaggaa ggtgctcttg ccggaaccgc tcgagccgat gatgctgatg acatcgccgg
    19741 cgccggcctg cagcgacacg ccgtcgagca cctgatgggt gccgaaggtc ttttccaggt
    19801 ttcttacgtt cagcttgggc atggggatcg ggcgtcagtg gcgcaacggc ttcaggtagg
    19861 ccagccagcg cgcttccatg tgacggaagc cgcatatcag cacaaaggcc aggcaggcgt
    19921 acagcacggc ggcgatgccg aaggcgttga aggcttggta ggtcgccgaa ttcacgtcgc
    19981 gcgccacttt caggatttcc ggcacggtgg cggtgaacgc cagcgaagtg gcgtgcagca
    20041 tcatcacgac ctcgttgctg taggccggca gcgcgcggcg cagcgccgag gggagtatca
    20101 cgcggcaata cagggtcgga ccggtcatgc cgaaggcgcg ggcggcttcg atctcgccgt
    20161 acggggtcgc cttgatcgcg ccggcgaaaa tttccacgtt gtaggcgcag acgtgcagca
    20221 cgaaagccag catggcgcaa ttcatgccgt cgcgaaagaa gctgctcaag aacggcgtgc
    20281 cgcgcacgaa gtccaggctg tagatgccgc tatagcaaat gagcagctgc acatacagcg
    20341 gcgtgccgcg gaagatatag gtgtagaacc agacagggcc gcgcagccat ttcctgctcg
    20401 acgcccgcgc gacggccagg ggcacggcca gcacgaagcc cagcacggtc gataccacca
    20461 gcaaccacaa ggtgacggcg acgccggtcc agtggtagcc atcggaataa agataattgc
    20521 gccagtattc ctggatgatc ccgatcacag ctcggactcc ctcacgccga gcgaatagcg
    20581 tttttccgcc cacgccaaga ccaggctgga caccgtggtg atggccagga agatgccggc
    20641 cgccaggacc aggaagaaga aggcgcgctg cgtgccctgg ctggcgtcga tggccgcctt
    20701 gacgaggtcg tccagcccaa ggatggtgac gatggccgaa gccttgacca ccaccagcca
    20761 attgttgccg atgccgggca gcgcgaagcg catcatctgc ggaaaaagaa tatcgcggaa
    20821 cacccgccag cccgacatgc cgtaggcata tcccgcctcg atctggccgc gcggaaccgc
    20881 caggaaggcg ccacgcagcg tttcggtgta gtaggcgccg tagatgaagc ccagcgtgat
    20941 cacgccggcc aggaagggcg ggatgtccat ctgctgcaag cccaggctct cggtgacgtg
    21001 gttgagcccg atctggatgc cgtagaagag cagcagcatc agcaccagat cgggcaccgc
    21061 gcggatgagc gtggtgtagg ccgtggcgac ggcgcgcaag aggcggctgc gcgaaagctt
    21121 ggcgctcgcg cccagcaggc ccagcaggaa ggccacgacc agcgacagga cgccgagctc
    21181 gatggtcgcc caggtgccct cgagcagaag ccggccatag ccatccaggg aaaaaaatcc
    21241 agccatgcct ttattcgtag ggcgcgaatt gttcgctgcg ccacttcagc gcgggaccca
    21301 ggccttgctc cttgcggatg gacatgaatt tggccttttc ttccgacccg gagaaattca
    21361 tcagcgtgca ggcctcggcg ccgtattcga tggcctgccg cagccccatg atctcgaagg
    21421 tctggttcag cgcccgcttg tcggcctgca ggcattccag cgacacgcgc gacatccgcg
    21481 gcgcgatctt gcggacttcc gcctgcacgg tttccttgga gaagaccttg tcgaccaggc
    21541 ccaggcgcca ggcttcggcc gcatcgatgg tgtcgccgct gtagatcagc gcgcgagcgc
    21601 gctggccgag cagccagggc atgatcatgg tcgagatgcc ggtggaaaaa cgggcttcca
    21661 gcgcggcgaa cgtggacggc tccgaacaat agcgcatgtc gcagcacatg gccagctcca
    21721 gcgcgccggc caggcagaag ccgtcgatca gcgcgatcac gggcttgctg caatcccaca
    21781 ccgaataggt gaagtggatg tccttctgca tgcgggcgcg catctgcggc aaggtcgtga
    21841 tgggcgcggc ggtggtctcc ttcatgtcgt agcccgacga gaaggccttg ccgcccgcgc
    21901 cggtcacgat cacgacgcgc acttgcgggt cggcgtcggc ctgggcgacg gcctgggcca
    21961 gttagtccat caattgcagg ctgaaggcgt tgagccgctc ggggcggttc agcgtgatgg
    22021 tggccacgcg gtcttcgacc gcgtaaagca gcgtgtcgta gttcatatcg aattttcctc
    22081 ggcaggggta tggggttcga gcaggaggtc gacgcccacg gcgccgtgtt cctggacgat
    22141 cagcgggttg atttccagcg cggccagcgt gtcccggtgc gcatggccga aacgcgagaa
    22201 cgcggcgatc gcctcggccg cggcatcgat gtcccacggc ccctggccgc ggacgccgtc
    22261 gagcagccgt ccggcggcgg tctcgcgcag catggcgcgg gcttcttcgg cgctgatggg
    22321 acccagccgc atgccggcct gcttgctgat ctcgaccagc acgccgccgg gcccggcgat
    22381 gacgaagctg ccgaagcgcg gatcgttgcg cacgcccacg atcagctcca cgcccgagcc
    22441 ggccatcttc tgcacgacga tttcgccgga agcgcccggc agcgcctgtt cgtccaggat
    22501 ggcgcgcagg gccgcgtagg cctgcccgac cgcggccgcg tcgcgcaggc ccagccgcac
    22561 caggcccagg tcgctcttgt ggggcagatg gtcggccacg cccttgagca cgaccggata
    22621 gcccagccgt tgcgccgcgt cggcggccga agccgcatcg tccacgcgtt cgctcggcgt
    22681 catgggcacg ccggcatggc gcaaggcctg gaagcggccg ggttcgtcgc cgggcaggac
    22741 ggccagcggc tcgcccgccc gggcctcggg cctggcgacc ggcgcgtcga ccagattgcg
    22801 gatggccttc aacgcggtcg gcatgccgga caggtaggcg ataccggccg ggtcgagcac
    22861 ggcgcgcacc tcggcgttca ccccggtgcc ggcgatgttg ttgatgaata ccaggcgctt
    22921 gccgcttgcc gtcttggcca ggacctcggc gcagacccgg cccatgatga gggcgaaatg
    22981 cgtgtcgcag ccgccgtgcc cgggcgcgtc caccacgaag cccagggtgc cgaggccggg
    23041 gtcttccatc agggcccgca agatgatgcg gaagttttct tcgttgaaac ccaggcccca
    23101 gccatcgacg gggttggcca ccgtggaatg cggcggaaag gcggggcgca tcttggccag
    23161 cgtttcaggc gagagcggcg gataggccag gccgacgtcg tggccgatgt cggccagcag
    23221 cgcggcctcg ccgcccgaca gcgtcatgat ggcggtgccg caagccggcg gggcggcgca
    23281 gctggagacg aacaagctgg cggtttccag catttcgtcc aggtcgcgca cccgcacgat
    23341 gcccaggtgc ttgaagaagg cgtcgtacag ccggtcttcg ccggccaggc tgccggtgtg
    23401 cgcctgcacg agcgcccgcc cgccttcgct cgtgcccagc ttgagcgcga ggatgcgctt
    23461 gccgcgccgc gcggcctcgc gggccgcgct cgccagcagc gccggatcgc gcacggtctc
    23521 caggaacagc aggatcacct ggacgttgtc gtcgcgcacc atcacgtcca ggtagtcggc
    23581 caccgtggcg ccggcctcgt tgccggtggt gaccagatag gcgaaaccta gctcgcgttc
    23641 gtcttcggcc agcgccatgg ccaccgaccc gctttgcgag atcaggccga cagggccggg
    23701 cttgttcatg cgcatgtgca tgggcgtcca catggccgtg cgcgcatgga tgttcaccag
    23761 gcccaggttg ttgggccccg acaacgccat gccatggcgg cgcgcgctgt cgacgatgcg
    23821 gcgctgcaag gccttgccgt gctcgtcgcc gtcggcgtag ccgttggcgt tcagaaaggc
    23881 ggcgccgatg cccagcctcc ccgcttcctc caccaagtcc ggccccgccg ccgcgggcac
    23941 ggccaggaag acgacgtcca ccgtttcagg cagggccgcg aggcccgggt agcacgccgc
    24001 gccgtcgaag gtttcatatt tcgggttgac gggatagacg ggccccggga accccagctt
    24061 gcgcagattg ttccagaccc gggtgccggc ggcgccagga cgctccgata cgccaacgat
    24121 ggcgatggaa cgaggacgca tcagttttgt tagggaattt gccaagatag agattacccc
    24181 ttgcatgcta ttgtgttttg aacctgacta tacaggctta gacatctgct ttgaccacga
    24241 ctaggggaaa acgatggaaa tcaagccgct catcggcgcc ttgggtgttg aagtgctcgg
    24301 catggacacg tccgcgccga gcgatgaact ggtgcagcag atgcgttcgc tgctgtggga
    24361 ataccaggtg ctggtgatcc gcgaccagaa gctcagttgc gaacagcaca ttgcgctggc
    24421 caggaaattc ggcccggtgc acgcgcagcc gttcaactat gcgaaggcct tgccgaatca
    24481 tccggagata ctgccggtca tcaaggaaaa gaccgacacc aaggtcttcg gcgaaaaatg
    24541 gcacgccgac gtcacctacc tccagcgccc cttgctgggc gcgctgctgt acgcgctcga
    24601 aaccccgccg cgcggcggcg acaccctgtt cgccaacatg taccgcgcct acgatgcgct
    24661 gtcggacggc atgaagcaaa tgctggcagg cctgacggcg gaacacgagg gctcggtgcc
    24721 cgacggcgat ccggtcacgg gcgagcgcta cgagcccatc cgcctgatcc accatcacgg
    24781 cgagcacccg gcgatcacgg tgcaccccga gaccggcaag aagctgctct acgtcaacac
    24841 gacctacacc agacgcttca agggcatgac cgacgccgag agccggccct tgctggaata
    24901 cctctgcaac cacgccaccc aggccgagtt cacggcccgc gtgcactgga agccaggcac
    24961 gctggtcatg tgggacaacc gcagcacgca gcacctgccg atcaacgatt actacggcta
    25021 ccggcgcgca atgcaccgga tcacggtatc gacctgagac aaaccctgat tagggccaac
    25081 ctgtataggc atgtatagga tttatacgta gcatctggag tgccggctgg catcgacccg
    25141 tccggcccaa cacaacacga catctggcac aacgctgcca taccaagggg aagcaccatg
    25201 aaatccaagc cgcagcacct gctgttccgg tttctggcgg gcatgacgct cgccaccgcg
    25261 tcgcacctcg cgatggccgg cgtgctcgaa gatgccaagg cccgcggcga actcgtcgcc
    25321 tgcaccgaat tccagttcgc gccgttcgag ttcctcgatg gcgacaagcc cgtgggcttc
    25381 gacatcgatc tcgtcgcgct catcgcccag gacatgggcg tgaaggtcaa gtacatcgac
    25441 ctgccctggt cgagcgtgct gcccgcgctg gaatccaagc gctgcgacct ggtgctggcc
    25501 ggcgtcatgc gcaccaaggc ccgcatggag cgctacgaca tgtcgctgcc gatcggcgac
    25561 gccatcgtcg ccctcgtcaa gcggcacggc gacgccagca tcatgaagcc cgaggacatt
    25621 gccggcaagg tcgtgggcgg cgtcaagggc tcggcccaga tccagatctt ccagaacttc
    25681 atcgacacca aggtgccggg cggcgtgaag gaactcaagg cctacaccgc cagcacccag
    25741 tcctatgccg acctggccgc cgggcgcatc gtggtcgcgt ccgccgcgat ggccaatctg
    25801 gcctacctcg tcaagacccg cccggaattc gagatcgtgg agcccggctt cgggccgccg
    25861 acctatttcg gcttccccat ccgcaaagag cccgagagcc agaccctggc cgccgccatc
    25921 aacgccggca tcacgaagta caacaaggac ggcaccatgg ccaagctgca ggagaagtgg
    25981 ttcggcaagg ccttcaccct accctacgaa aacgtccccg agccgacgat ctgaccgcgg
    26041 gccgcgcatg gtacccacta ccggcgtcac gtctttcctg accggcatcc acctgctgct
    26101 ggtgggggcc ggcgtcaccg cgggcatctc gatcgtcgcg atctgcctgg gctttctgtt
    26161 cgcctcgctg gtgtgcgtgg cctgcatgtc caggagcagg cccctgcgtt tcgccggcga
    26221 ggtctatgtc agcttcttcc gcggcgtgcc gctgctcatc cagctgctgg tgctgtatta
    26281 cctgctgcct ttcatcgggc tggacctgcc gcccgtcgtg gcgggcacgc tggggctggc
    26341 gctgtgcacc tcggcctacc aggccgaaaa cctgcgcggt ggcctgctga ccatcccggc
    26401 tggccaggaa gaggcggcgc gcgccttcgg ctacaacgtc gtgcagcgct ggacgcgcat
    26461 cctgctgccg caggccctgc gcctttccat gccgatgatc gtcaacgaaa tgatcgccat
    26521 cctcaaggcc tcctccctgg tgtcggtggt cggcatcacc gatttgaccc gcgtgtcgca
    26581 gaacatcgtg gcccgcaact tccagcccat ccagtggtac gccacggccg cgctctttta
    26641 cctggcgata aacctggtgc tgtcgtattt cagccgggta gccgggcggc gcctgggccg
    26701 cagccacagc ggggccatgc tatgaagctc gatttttccc tattgctcca gttcggcccc
    26761 gatctgctgc agggcttttt caacacgatc aagatctggc tctccgcggc gctgctggcg
    26821 gcgctcatcg gctcggtgct gggcttcgcc atgaccgtcg gcccgcgccg gctgcgccag
    26881 gccattacct gctacgtgga gtttttccgc tgcacgcctt tcctggtgca gttgttcctg
    26941 ttttatttcg gcgggccgag catcggcctg acgctggacg cctaccccgt cggcatcatc
    27001 ggcctggggc tatatggcgg cgcctatttc acggcaacga tgcatgcggg cttcaacagc
    27061 atccccaaag gccagatcga ggccgcgcag agcttcggca tctcgcgctg gcagacggtg
    27121 cgccatgtcg tcctgccgca gatgctggtg ctcatcatcc cgcccggcat caacctgctc
    27181 atcttcatga tcaaggatac ggccgtcttg tccatcatca cggtgcccga gctgaccttc
    27241 cagatcacca acatcaccca ggaaacctat gcattcgtcg agccttatct cgcgctggcg
    27301 ctgggttatt gggtgctggt cgaaatcacg gcgcgactgg gccgcctcgg cgaatccctg
    27361 gccacccgcc atcttggaaa agccgcatga ccgcttcacc caataccgcg ccgcaagcgg
    27421 cctcgcccct catcgtcatc gaaggcctgt ccaagtccta cggcgaccac ccggtgctgc
    27481 acgacgtgtc gctgacgatc aaccgatccg aggtggcctg catcatcggt ccctcgggct
    27541 cgggcaagag cacgctgctg cgctgcatga acttcctgga agacttctcc gcgggcacca
    27601 tccacatcga cggcaagccg ctgggctacc gcaagacccc ggacggcagc cgcgcggcga
    27661 cctcgcgcgc ggcgctcgag caggcgcgcg gcggcgtggc gatggtgttc cagcagttca
    27721 acctgtggcc gcacatgacg gtgctgcaga acgtgaccca gccgctggtg ctggtgcacg
    27781 gcgtgcccgc cggccgcgcg cgcgagacgg gcatgaagat gctcgagcgc gtcgggctgg
    27841 cccacaaggc gcaggcctac cccggcaatc tctcgggcgg ccagcagcag cgcgtgggca
    27901 tcgcgcgcgc cctggccatc gacccctcgg tcatcctgtt cgacgagccg acttcgtcgc
    27961 tggaccctga actcgtgggc gaagtgctgc aggtcatcaa gcagttggcg aaggacggcc
    28021 tgaccatggt ggtcgtcacg cacgaaatgg gcttcgcggc ccagatggcc gaccgcgcga
    28081 tcttcgtgga tcatggcgcc atcgtggagg aaggaccgcc gcgcacgctg ttccgcaacc
    28141 cgaagagcga gcggctgcgg caattcctgc aaacctggat ggaacgcagc cagctgttcc
    28201 aggacgcgcc gtaaagcgtt tttcccgggt tataaaggtc ttttggatga cagccttcat
    28261 gacccgttct caactttccc gccccgtggg catcaccatg ggcgacgcgg ccggcatcgg
    28321 gcccgaaatc gtcgtcaagg ccttcgcaca ggggctggcc gcgccggcca tcgtctatgg
    28381 cgatgccggg gcgctgcgcc gcgccgcggc gctcgtgggc gcgccgctgg ccatcaacga
    28441 gatccactcg cccgacgcgg cgcgctgctc ggccgaggcc atcgacgtcg tggccagttc
    28501 gcccgccctg cccgccgacc ttcccgccgg caaggtcagc gccgcggcag ggcaagggtc
    28561 ttacgactat ctctgcgccg ccatcgacga cgccatggcc ggccgcatcc gggccatcgt
    28621 cacggcgccg ctgaacaaat acgccatgca cctggccggc atcgaccagc ccggccacac
    28681 cgagatcctg gccgaacgct cgggcacggc cgactacgcc atgatgctcg ccaacgacga
    28741 gttgcgcgtg ctgctggtga ccatccacgt ggccctgtcg caggtgccgg cgctcatcac
    28801 gcccgcggcc gagctgcgcg ccatccgcct ggccgaccgg gcctgccggc agatgggcgt
    28861 gggcttgggc gacaagccgc cccgcatcgc cgtggccggc ctgaaccccc acgccggcga
    28921 agacggcaag ttcggccgcg aggacatcga cgccatcgcg ccggccatcg cgcaggcgca
    28981 ggccgaaggg ctggacgcct ccggcccctg gcccggcgac accgtcttca tgcgggcccg
    29041 gcgcggcgag ttcgacatcg tcgtggccca gtaccacgac caggggctca ttcccgtcaa
    29101 atacctgggc gtggaccacg gcgtgaacgt caccgtgggc ctgcctttca tccgcaccag
    29161 cgtcgaccac ggcacggcct tcgacatcgc ctggcagggc aaggccgacc aggcctcgct
    29221 ggtggaggcc ttcaacctgg ctatcaagat ggcgtaaggt cgggcccggg acctgggcgc
    29281 ctctcgcatt ccggcgccag gcctctacaa tgctgggtta ttccatagct ctcccaccaa
    29341 ccccgccatg caacgcctcg cccgctttct acctgaacgc ttcacctgcc tgatccttct
    29401 caccgtcctc atcgccagcc tgctgcccgc ccacggccag ggcatcaaga tcgtcggcaa
    29461 catcgccaac ctggccgtcg cgctgctctt cttcctgcac ggcgcgcgcc tgtcgcgcga
    29521 ggccatcgtg gccggcttca cccactggcg cctgcacctg acgatcttct cgtccacctt
    29581 cctgatgttc cccatcctgg gcctgctgct caagcccgtg ctcgagccgc tggtcacgcc
    29641 cgagctctac atgggcatcc tgttcctgtg ctgcctgccg tccacggtgc agtcgtccat
    29701 cgccttcacc tccatggcgc gcggcaacgt gcccgccgcc gtgtgcagcg cctcgggctc
    29761 cagcctgctg ggcatcttcc tgaccccgct gctggtcggc gtggtgctgg cgggcgggtc
    29821 cagcgcgccc atgtccttcg gcgcggtcga gaccatcatg ctgcagctgc tgctgccctt
    29881 catcctgggc caggtcctgc gccgctggat cggcggctgg gtgcacaggc gcaaggccat
    29941 gctcaagtgg gtcgaccagg gctccatcct gctggtggtc tacaccgcct tctccgaggc
    30001 cgtgagcgaa ggcctgtggc acaacacgcc catcctgtcg ctggtcgggc tggtggtctg
    30061 caacctcatc atcctggccc tggccctgct gggctccaac accatgggca aggtcttcgg
    30121 cttctccctg cccgaccgca tcaccttgct cttctgcggc tccaagaaga gcctggccac
    30181 gggcgtgccc atggcccagg tgatcttcgc cggccaggcc gtcggcgcca tcgtgctgcc
    30241 cctgatgatc ttccaccagg tccagctcat ggtctgcgcc gcgctggcga gccgctatgc
    30301 gaagcgcccg gaagaaccgg gcgaacacgc gcaccattga aaaaagggcg tcccgcggga
    30361 cgcccttttc ttaacggcag gctcaatatc ccgccgccga gaaattcagg aaccgcgtgc
    30421 tggaaccctg gaaggtcagc cgcaccgttc cgatggggcc gttacgctgc ttgccgatga
    30481 tgatctcggc cgtgcccttg tccggcgaat ccgggttgta gacctcgtcg cggtagatga
    30541 acaggatcac gtccgcgtcc tgttcgatgg cgcccgattc gcgcaggtcg ctcatcaccg
    30601 gccgcttgtt gggacgctgc tccaggctgc ggttgagctg cgacagcgcg atcagcgggc
    30661 aattcagctc cttggccagg cccttgagcg agcggctgat ttccgagatt tcggtggcgc
    30721 ggttttcgct gttgccgcta ttgcccgaca tcagctggat atagtcgatg atgatcaagc
    30781 ccatctgccc gcattcgcgc gccaggcggc gggcgcggct gcgcacgtcc atggccgaca
    30841 gcgccggcgt ctcgtcgatg tagagcaagg cgtcctgcat ttgcgacacg gcatgcgtga
    30901 cgcgcggcca atcctcgttg gtgagcttgc ccgtgcgcat gcggtgctgg tccagcatgc
    30961 cgaccgagcc cagcatgcgc atggccaatt gcaccgcgcc catttccatg gagaacaccg
    31021 ccaccggcag cccctgttcg atggccacgt gttcgccgat gttcatcgag aacgaggtct
    31081 tgcccatgga gggacggccc gccacgatga ccaggtcgcc cgcctgcagg cccgaggtca
    31141 tcttgtccag gtcggtaaag ccggtgggca cgcccgtgac gtcggaatcg ccttcacggt
    31201 gataaagctc gtcgatgcgc tcgaccactt gcgacaagag cggctggatc tgctggaaac
    31261 ccgccaggcc gcgctggcct tcctgggcga tctgcagcac cttggactcg gcctcgtcga
    31321 gcagttgctt ggcttccttg ccctgcgggt tgagcgcggc cgacgagatc tcgtcggcga
    31381 tcgacaccag gcggcgcagc atggcgcgct cgttgacgat ctcggcgtag cgacggatgt
    31441 tggccgccga cggcgtgttg tgcgccaacg cattgatata ggccaggccg cccacttcct
    31501 cggccttgcc gctgctggtg agcgattcgt gcaccgtgat cacgtcggcc ggacgcgaga
    31561 gctcgatgag ccgggcgatg tggtgccaga tcaggcggtg gtcgtggcga tagaaatcct
    31621 cctcgcgcag cgtgtcgctg acacgctccc aggcggcgtt gtccagcaac aggccgccca
    31681 ggacggactg ctccgcctcg atggaatgcg gcggtacgcg caggtattca agctgggaat
    31741 cggcaggggt attcatgcgg caatagtaac cttggcgctc ttcttggact gtaaaaagcg
    31801 ctgtcaaaac actaacgcaa aaacaagccg gccgggcgtt gccacccggc cggctgtttg
    31861 tctggctggt gccgggctcg cgctcggcgc ctggccaact caggccatgt cgccttcgac
    31921 cagcaccgac acctcgacca gcacgtcggg atgcagggcg acctggaccg aggactcgcc
    31981 gacggtcttc agttggccgg tgggcatgcg gacctgggcc ttggccacgc cttcgaaacc
    32041 cgccttgtgc aggccttcgg cgatgtcggc gttggtgacc gagccgaaca ggcggccgtc
    32101 gacgccggcc ttctgtacga ccttgagctg gtagccggcc aggcgttcgc ccagggcctg
    32161 ggcagtggac agcttctcgg cctgggtctt ttccagttcg acgcggcggg cttcgaattc
    32221 cttcagggcg gcgtcggttg cgcgacgggc cattttctgg ggaatcagga agttgcgggc
    32281 atagccgtcg cgcacgcgca cgacttcgcc gaggttgccc aggttgacga ctttttcaag
    32341 caggatgact tgcatttcag ggcccccgat cagttatggt tgtcggtgta gggcagcagg
    32401 gccaggaaac gggcacgctt gatggccgta tccagttggc gctgatagat cgcccgggtg
    32461 cccgtcagac gggcgggaat gatcttgccg ttttcctgca cgaagtcgcg cagggtgtcg
    32521 agatctttgt aatcgatttc ttcaacgccg gcggcggtga agcggcagaa cttgcggcgc
    32581 ttgaagagcg ggttctgctg ggtgaatttg cgcttttctt tcttcttccc aaaagccatg
    32641 atgtgcctct tgaatttgat ctatctgtga ttgatatcga gcttgcgacg tccaggtccg
    32701 gcttcaggcc accatcgggt cgctgccggc gctgccgctg atccgtcccg cccgctgcat
    32761 gtgcagcttg agttttgccg aatccttgcg caccggggcc aggaagccct ccacgcgcag
    32821 ttcggcaccc agggggatgt cggacaacag catcgccaga tcgcccaagg ccaccgcgtt
    32881 caccgtgaag gcaacccgcc tgggctgccc ggcttcgatg acctccgatt cgtgctccag
    32941 cagcatttcg atcgccggca ggccggcggg ggtgtgtcgc aagggctcgc gttcgatgac
    33001 gcgcgcgacc agctcaacct tgttcatgct gtgtgcccga atgacgggga ctgttttggt
    33061 tgtcgctcgc tttccgttgc ttattccgtg accgcggcgg ccggggccgt ttcagcggca
    33121 gccttgcggg cctcttcacg ctcgaccgac ttcatcatga tcgagggcgc ggtgacggcc
    33181 ttcttggtct tgatgaccag gtggcgcagc acggcgtcgt tgtagcggaa cgagtgctcg
    33241 agttcgtcca tcgtggcctg gccggcttcg atgttcaggc aaacgtaatg ggccttgacg
    33301 agcttctgga tcgggtaggc cagttggcgg cggccccagt cttcgaaacg gtgcacggca
    33361 ccgccttgac cggtaaccag ggcttggtag cgctcgacca tggcgggcac ttgctcgctt
    33421 tggtcgggat gcacgataaa caccacttcg tagtggcgca gggtttgcgt catgaggtaa
    33481 ctcctatgga tgtctcgcca agcccaacct catgggtggc gaggggcagc ccgctgtcca
    33541 atttcttggc agtcgagcaa gtaagccttt gattttcgct tggattgcga aaatttgcaa
    33601 gttaatcagg ttcttaaggg cgtctggcga gggcaaagcc cccatccggg cttcgctgcg
    33661 gcgggctcgg gcgcgccatc aggcgccctc ggccccttcg gggctgcccc gccgcagagg
    33721 cgccctcctg ggggctttgc ccccgccaga cttaggcctg cctactgcct ttcaaccttc
    33781 gaccacggcg taggccgagt ggttgtggat cgattcgaag ttctcggcct cgacgcggaa
    33841 gcgccggacg ccggcgtggg cctgcatgcg ggcggccacg tcgcggacca ggtcttcgac
    33901 gaacttgggg ttgtcgtagg cgcgctcggt gacgaacttt tcgtcggggc gcttgagcag
    33961 cccccacagt tcgcaggaac cctggctttc gatttcgcgg atgatctcat ccatggaaac
    34021 ggcgccgtcc agcacggccg acacggtgac gtgcgagcgc tggttgtggg cgccgtactc
    34081 ggaaatggcc ttggagcatg ggcacaggct ggtgacgggc acttgcacca cgagctcgaa
    34141 ctccacgccc gtctcggcgg cgcgggcgac ccactggact tcgtagtcga gcaggctttg
    34201 cacgccggag accggcgcgg acttgttgat gaagtacggg aaggcggccg tgatgtcgcc
    34261 gcgctcggcg tgcagcaggg acagcatttc ctgggccatt tcggcgaaca gggccggcgt
    34321 catggccgtg gcgcggtgct tttcgagcaa ggccacgaag cgggacatgt gggtgccctt
    34381 ttcttcggcg ggcagcgcca cggtgagcgt ccagttggcc acggtaggct gggccgagcc
    34441 gtcggccgcc ttgaccagca tgggatggcg cacgccgcgg atgcccacgc gttgaatggg
    34501 gatgtgcctc gtgtcggccg tgctttgcac gtcaggcatg acgatggcgg gatcgattgg
    34561 agaattcatg tcggggacct gtatcgcggg cgtaaagggt gcccgccgga atcttaccgg
    34621 gatgtcatat tatcccttat tccgagtaaa tcgttcggga aaacccttct gacagccggg
    34681 acggattgtt ccatccgccc tataaaaacg cgctgaaccg ggcccggatg gatgcctcga
    34741 tgcccgcggc atccaggccc agcccggtca gcagcgccgc ctggtcgccg tgatcgatga
    34801 aaaggtccgg caggcccagc tgcagcagcg gcttggccac gccggcctgg ctcagggcct
    34861 cggccacggc gctgcccgcc ccgcccatga tggcggcctc ttccagcgtg accagggcgt
    34921 cgtggcgggc agccaggtcg agcaccagtt cgcggtcgat gggcttgacg aaacgcatgt
    34981 cggccaccgt ggcgcccagg ctctcggccg ccttcagggc ggccggcacc agcgtgccga
    35041 aacccaggat ggcgatacgc gcgccttcgc ggcggatcac gcccttgccc agcggcacgt
    35101 cgtcgaaccc ggccgtgacc ggcgcgccgg caccggcgcc gcgcggatag cgcaccgagg
    35161 ccgggccggc gtggcgatag caggtggtca gcagcaggcg cgcctcgttt tcgtcggacg
    35221 gcgtggccac caccatattg ggaacgcagc gcaggaaggc gatgtcgtag ctgccggcgt
    35281 gcgtggcgcc gtcggcgccc accaggccgg cacggtccag cgcgaaggtt acgtcgaggt
    35341 tttgcagcgc aacgtcgtgg atgagctggt cgtagccgcg ctgcaggaaa gtcgagtaga
    35401 tggccaccac cggcttctgc gcctcgcagg ccaggccggc ggcgaaggtg acggcgtgct
    35461 gttcggcgat gcccacgtcg aagtagcgtt gcgggaagcg ctgttcgaac tcgaccaggc
    35521 cgctgccctc gcgcatggcg ggcgtgatgc cgaccaggcg cgtgtcggcc tcggccatgt
    35581 cgcacagcca ctggccgaac acctgggtga aggtctgctt ggccggggcc ttggacttct
    35641 ggatgcccac gcgggggtcg aacttgccgg ggccgtggta gagcaccgga tcggcctcgg
    35701 ccagcttgta gccctgccct ttcttggtaa ccacgtgcag gaactgcagg ccctgcagtt
    35761 ccttgaggtt ttgcagggtg gggatgagcg cgtcgaggtc gtggccgtcg atcgggccca
    35821 cgtagtggaa gccgaactct tcgaacagcg tggccggcga caccatgccc
//
LOCUS       DASWPO010000055        23674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_11018, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000055 DASWPO010000000
VERSION     DASWPO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 23674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23674
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11018"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VGC69_03350"
     CDS             complement(<1..777)
                     /locus_tag="VGC69_03350"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0294355.1"
                     /translation="MSKQSFEAPAHIGQLPAYQAGKPIEELAREFGLDPAGIIKLASN
                     ENPLGMPASARAAIGDAAATLARYPDANGFDLKAALSARYDVPLDWLTLGNGSNDLLE
                     LAAMTLLQQGSSAVYAQYSFVVYRLATQARGARHIVVPARDHGHDLDAMFDAIADDTR
                     LVFVANPNNPTGTFLSGAQIEAFLAKVHAAHGERVTVILDEAYNEFLDPGLRFDSVAL
                     VRRYPNLVVSRTFSKAYGLAGLRVGYAIAQPGLTELFNRVR"
     gene            complement(847..1935)
                     /gene="pheA"
                     /locus_tag="VGC69_03355"
     CDS             complement(847..1935)
                     /gene="pheA"
                     /locus_tag="VGC69_03355"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY0294356.1"
                     /translation="MDDASLQARLGPLRARIDELDARILELLNQRARTAQEVGVAKQA
                     ARDGGPVLRPEREAQVIRGLQAGNPGPLPGAAIAAVWTEIMSACRGLERGMTVAYLGP
                     RGSFSEQAALEHFGHSVNRLPCGSFDEVFRAVEAGQADLGMVPVENSTEGAVNRSLDL
                     LLASPLKLLGERSLVIRHCLMTQSGTMDGVTTVSAHPQALAQCQGWLAHHYPDLHTVA
                     AASNSEAARVASEDGSVAAIAGEIAAASWGLSVVESGIQDDAQNRTRFLAIGHIQPAA
                     SGRDKTSLILAVPNRAGAMHEMLAPLADNGVSMTRFESRPARTGQWEYYFYIDVEGHR
                     DDPAVAKALAALQRQVAFFKLLGSYPAQ"
     gene            complement(1928..3058)
                     /gene="serC"
                     /locus_tag="VGC69_03360"
     CDS             complement(1928..3058)
                     /gene="serC"
                     /locus_tag="VGC69_03360"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY0294357.1"
                     /translation="MARPWNFSAGPAAIPEAVLQQAASEMLDWHGSGMSVMEMSHRGR
                     QFVQICDEAENDLRELLALPADYAILFMQGGGLGENAIVPMNLLGRRASPAADFLVTG
                     HWSVRSHKEAGRYGDARIVASSGQAAMIDGREQSAWTWVPPTDTWSINKDAAYLHLCS
                     NETIGGVENLDWPDMAALDAPDVPLVVDASSHFLSRALPIEQAGLVFAGAQKNVGPAG
                     VTVVIVRRDLIGHALSICPSAFDYANVAAEHSRYNTPPTFGIYMAGLVFKWLKAQGGV
                     AAIEAANRAKAELLYGYLDGTGFYRNPVHVPMRSRMNVPFVLHDESLNEVFLKGAEEA
                     GLMQLKGHKSVGGMRASIYNAMPFEGVQALVDYLQAFERRHG"
     gene            complement(3070..5718)
                     /gene="gyrA"
                     /locus_tag="VGC69_03365"
     CDS             complement(3070..5718)
                     /gene="gyrA"
                     /locus_tag="VGC69_03365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0294358.1"
                     /translation="MDSFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMHELNNDWNRAYKKSARIVGDVIGKYHPHGDQSVYDTIVRMAQDFSMRYML
                     VDGQGNFGSIDGDNAAAMRYTEIRLAKIAHELLADIDQETVDFGPNYDGSEQEPLLLP
                     SRLPNLLVNGSSGIAVGMATNIPPHNLAEVVDGCLYCLRNPDCSIDELIELIPAPDFP
                     TGGIIYGISGVREGYRTGRGRVVMRAKTHFEDMEKGNRQSIVVDAIPYQVNKKSLQER
                     IAELVNEKKIEGISDIRDESDKDGMRLVIELKRGEVPEVVLNNLYKNTQLQDTFGMNL
                     VALVDGQPRLLNLKQMVEYFLSHRREVVTRRTVYQLRKARERGHVLEGLAVALANIDD
                     FIAIIRAAPTPPVAKQDLMARSWDSSLVREMLVRADGGDTPGGRVAYRPDDLDPAYGL
                     QDDGLYRLSDTQAQEILNMRLQRLTGLEQDKIIGEYREVMETIADLLDILSRPERITA
                     IISDELQAIKSEFSAGAKDKDLRRSEIETNATEIDTEDLITPMDMVVTMSHSGYIKSQ
                     PLSEYRAQRRGGRGKQATAMKEDDWIDQLFIANTHDFLLCFSDRGRVYWLKVWEVPSG
                     SRGSRGRPIVNMFPLADGEKITVVLPVKVFGDDHYVFMATSRGTVKKTPLSDFSNPRK
                     AGIIAVDLDDGDYLIGADLTDGKHDVMLFSDSGKAVRFDENDVRPMGRNARGVRGMML
                     EDGQTVISLLVAGDESQSVLTATENGYGKRTSIVEYTRHGRGTKGMIAIQTSSRNGKV
                     VGAVLVNPADEIMLITTGGVLVRTRVSEIREMGRATQGVTLISVDDGSSLSGVRRVVE
                     SDADEDENADEGPAQDDGAQAQEGTE"
     gene            6143..6733
                     /locus_tag="VGC69_03370"
     CDS             6143..6733
                     /locus_tag="VGC69_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0294359.1"
                     /translation="MNKPSKFALALAFAAVTASGMASAQTGTRIDNWRDNFGDVWKNG
                     TREYCWRDNFWTPATGIQGCDGVASAPAPAKGAPVASKVVFNADAFFDFDKATLKPEG
                     RQLLDQVAAQIGTINLETIIAVGHTDSIGTVAYNQKLSERRAAAVKTYLVSKGIDANR
                     IYTEGKGKTQPVADNKTAAGRAKNRRVEIEIVGSRK"
     gene            6816..7553
                     /gene="ubiG"
                     /locus_tag="VGC69_03375"
     CDS             6816..7553
                     /gene="ubiG"
                     /locus_tag="VGC69_03375"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY0294360.1"
                     /translation="MSTDTSARNGTNGNVNADQAELDKFSALASRWWDPESEFKPLHA
                     INPLRLDWIREQVSSLAGLRVLDVGCGGGILAESMARAGADVTGIDLAEKSLKVAKLH
                     GLESGVKVDYRNIPVEQLATEQPGAFDVVTCMEMLEHVPDPASIVRACATLAKPGGWV
                     FFSTLNRNPKSFLFAIVGAEYLLKLLPKGTHSYASFIKPSELAAFTRQAGLAAVELKG
                     MEYNPVTQVYSLTGDTSVNYLVATRKQ"
     gene            7570..8235
                     /gene="gph"
                     /locus_tag="VGC69_03380"
     CDS             7570..8235
                     /gene="gph"
                     /locus_tag="VGC69_03380"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939497.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY0294361.1"
                     /translation="MSALVLFDFDGTLADTAPDLAAAANAQRARRGMAPLPVDVLRPV
                     ASQGARGMLRVALDMLPSHPEFEAARQQFLDHYAANSTVHSRLFPGIAELLERIRAQG
                     HAWGIVTNKVAHLTRPIVEHLGLARHCATLVCGDTTPTPKPHPAPLLHAAQEAGFGVE
                     HCLYVGDDLRDVQAAHAAGMPAVAAAYGYLGDGGAVDTWQADAQAKNVAELWPAIAAL
                     LPK"
     gene            8450..9013
                     /gene="ahpC"
                     /locus_tag="VGC69_03385"
     CDS             8450..9013
                     /gene="ahpC"
                     /locus_tag="VGC69_03385"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEY0294362.1"
                     /translation="MSLVNTQIKPFKAQAFREGKFIEVTEASLKGKWAVVVFYPADFT
                     FVCPTELEDLADNYAEFQKLGVEVYSVSTDTHFSHKAWHDTSAAIGKIKYTMIGDPTW
                     QLATNFDVLREGQGLADRGTFIIDPTGVIQAVEVTAEGIGRDAKELLRKVKAALYVAA
                     HPGEVCPAKWQEGEKTLAPSLDLVGKI"
     gene            9100..10689
                     /gene="ahpF"
                     /locus_tag="VGC69_03390"
     CDS             9100..10689
                     /gene="ahpF"
                     /locus_tag="VGC69_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEY0294363.1"
                     /translation="MLDATLKSQLQTYMQMISQPIEIVASVDDSEKSRELTELLHEIA
                     AMSPNISYTERRDDNERKPSFSISRPGQDLGIRFAAIPLGHEFTSLVLALLQAGGHPL
                     KLDADVIEQIKNLDGDYTFETYVSLSCQNCPEVVQSLNVMSLLNPRIRQVTIDGALFQ
                     DEVETRQIMAVPTIYLNGENFGQGRMTVADFLAKLDANASKRDADKLNARKPFDVLIV
                     GGGPAGAAAAVYAARKGVRTGVVAERFGGQVLDTLAIENFISVTETEGPRFAAALEEH
                     VKAYDVDIMNTQRAEALVPGADYIEVKLANGASLKSRSVILSTGARWREVNVPGEREY
                     RNHGVAYCPHCDGPLFKGKRVSVIGGGNSGVEAAIDLAGLTEHVTLLEFGDALRADEV
                     LQRKLRSLPNVTVLTQAQTTEITGDGNKVNGITYKDRQTGETHHIELEGVFVQIGLVP
                     NTEWLKGTLALSKFGEIEIDPRCQTSLPGVFAAGDVTTVPYKQIIIATGEGAKAALSA
                     FDHLIRTPVPEQVEQQEAVAA"
     gene            complement(10776..11675)
                     /locus_tag="VGC69_03395"
     CDS             complement(10776..11675)
                     /locus_tag="VGC69_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294364.1"
                     /translation="MNITLRQLQAFIAVARSASFSRAAEAMSVTQPALTLMIQKLESQ
                     LGIQLLERRTRSVRLSPSGREFLPTIQRLIGELELTVDGLRDATLPRGGSLAIGCIPS
                     AGAIALPRLIKRFSQMYPHVRISVKDAMTETRDMIQMLRRGEIDLAMGAPSSEDSDIT
                     FEKQAQDRMVAVLPAEHRLAHRKTLGWRELGAEPLVVMSYHSHVRQLMDSAFSANGLS
                     VRPYAEVSLVATAVGMARSGLGAAVLPEQAVRMCNEEGLAVVPLVKPAVMRQVGFLYR
                     SFALLPPAAQAFVRMARDAAAKA"
     gene            11778..13562
                     /locus_tag="VGC69_03400"
     CDS             11778..13562
                     /locus_tag="VGC69_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase catalytic subunit"
                     /protein_id="HEY0294365.1"
                     /translation="MSGHDNTPSTGTWARRDLSGFLAADAMAAVLKAAEVPFIFGQSC
                     PTALFLAAAKLGIGQIGYRTENAGVAMADGYARASGKVGVITAQNGPAAGLLVAGLCE
                     AQKASVPIVAIVQDVPRANTDKNAFQEIDHEQIFRGCAKWVRKVVDPARAGEYMMRAI
                     MAATAGRPGPAVLLVPFDVLLEQVAPVTPDRPLADGPLPAGFGHYPVDRHAPAPELMQ
                     LAAQWIADGERPLIVAGGGVHTAQAAATLAKLQSLWQVPVATTNMGKGAVDETDPLSI
                     GVIGNCMGTRSPFKHQRDYVQRADVVLFVGTRTNENGTASWSLFDRDAKFIQIDVDPE
                     ELGRNYRGLRLLGDADTTLQALLGATPPALAERGHRNAPAIRQAISRARALSAAELAP
                     FEDSAQTPLRPERLFKELRACAPDALWVSDASYSSLWILQYLQARRSGDRFLTPRGIA
                     GLGWGYPMALGARLAQPARPVIAVVGDGGFSHCWAEIETARRHGIQAVVIVLNNGVLG
                     FQRDAEQSRFGVHTEICHFGPIDHVAIARGCGGDGIRVTQAEEIAPALARALAATVPM
                     VIDVDTAPEAFPPIQAFESLNADVAAQA"
     gene            13570..14355
                     /locus_tag="VGC69_03405"
     CDS             13570..14355
                     /locus_tag="VGC69_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294366.1"
                     /translation="MSGLSRQPSEQRGKTGLVTGAAGGIGLRTAERLALAGATVLMVD
                     QNQPALQQRAQALATLGYRVQALALDVADVHAAQRVEQALAELGTDAVDYLVNCAGIS
                     PKADGRRVPAWQVDPAEFQRVIAINLCGYFNMARAVAPGMIQTRRGAIVNIGSLAGLR
                     YSSIAGVAYATSKAGVTGLTQQLAGELAPFNVRVNAVAPGRIESPMSAMAAAGINDAI
                     LAGTPLGRLGTPDDIVDSVFFLLSEQAGFITGQTLAVTGGRGL"
     gene            14388..15359
                     /locus_tag="VGC69_03410"
     CDS             14388..15359
                     /locus_tag="VGC69_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294367.1"
                     /translation="MRNKTLAGLLTGLFLALGATPVLAGYPDEPLRLVVPYPPAGITD
                     IAARALAKGMSADLKQNIIVENRAGAGGSIGTAWAAHAAPDGYTILMGTSATHGTNPT
                     TLASLNYDAVKDFAAVSLVASAPLVVEVNPSLPVHNIREFIDYLKKHPDSVFFASTGT
                     GGSVHLSVEYFKLKTGTSMQHVPYKGSAPALSDLVGGQVQVMFDNIPSSAPLIKGGKL
                     RALAVTSTTRIADLPDVPTVSESGVPGFETGSWVGIYAPAGTPPAVVERLNKAVNAAL
                     QDPAVRTVFANSGLIPKGDSSADFAAFTKREIDTWADVARQIHYVPQ"
     gene            complement(15353..16399)
                     /locus_tag="VGC69_03415"
     CDS             complement(15353..16399)
                     /locus_tag="VGC69_03415"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEY0294368.1"
                     /translation="MLPLPLAQSVARVFESAGTRAEIARQVADNLVEANLKGHDSHGV
                     GMAPRYVQAIAEGGLQPNADTAEVAVDQGAILVMDGRRGYGQVVGSHAMRLAEARARG
                     QGLAMLGLRNVHHLGRIGAYAEQLAAAGLVSLHFVNVSSHPSVVPWNGLEARFGTNPV
                     CIGMPSPTGDHVILDFATSRVAHGKARVAWLKGERVAGGSAMVDAEGRQTDDPSVLFT
                     EPKGAQLPFGEHKGAGLALICSLLAGSLIGGPTEQHGPQGAAIVNGLLSIVIDPAALG
                     AGRQLMDEIDPFVAWVKEAAIPGQAVKAPGDAERESRARRSREGIALDDGTLGQLREA
                     AGSVGLDPAALFPY"
     gene            16563..16985
                     /gene="ssrA"
                     /locus_tag="VGC69_03420"
     tmRNA           16563..16985
                     /gene="ssrA"
                     /locus_tag="VGC69_03420"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            17152..17880
                     /locus_tag="VGC69_03425"
     CDS             17152..17880
                     /locus_tag="VGC69_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294369.1"
                     /translation="MDNKNGTTIWLFGRGLSCNCGLTWTVPESWRSLPRDEQIERIKP
                     AIRTEMNSPNVDISSIRRLLDDLAAKTPDGQKHRFGTTNWDYLLQREILVRSHDAWDG
                     IQPVWLAESHVSHINGTAEELENNAHRSPFLLEEDAYDKRTKSVEADNFYNFMIGGWR
                     FVVVGMSFECEIDRFLLYSLGRVQDDLPIGESTWLVVNRNYEALEKTRTAIADHLPRA
                     KIVAVQSSFSDWQNNGYSGLWDMP"
     gene            complement(18798..19241)
                     /locus_tag="VGC69_03430"
     CDS             complement(18798..19241)
                     /locus_tag="VGC69_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0294370.1"
                     /translation="MKVLEPISDASILTQLLAVKLKATASRMAVLRAMQRAPERWTSS
                     EGLYRECIQHGEQISIGNLYRILNELAQHGLLVRETDDNGKRFYRIRPAGSNPALHKI
                     ECHTTGDTFQFVDETLREHVARALRDRSLLLTESVITVQVQCVRG"
     gene            complement(19238..20617)
                     /locus_tag="VGC69_03435"
     CDS             complement(19238..20617)
                     /locus_tag="VGC69_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0294371.1"
                     /translation="MKGHRVFRLAALAWALALGGCGLPIAHTDAGLDLPGNWQATSVP
                     GEPVDADWWRGFGSAEMAGLVETARAGSFDVAAAAARVRQADALARMAGAALLPEVDG
                     TASVERSRTVGYPGLTAYTAGLSASYELDVWGANRAARQAAVDVLVATSYASDAVMLT
                     LTAGVADTYLRTLALRERVAIARQNLAAGERILTFVESQYRAGAATALDLAQQRTLVA
                     QLRQAVALRAQQAHDSRIALATLIGRPLQEVVVAGSGLDGVQTPSADAGVPANLFSHR
                     PDVAQAERQLATADADVTVARAAMLPSVTLTASAGVGNDRLRGLFDHPIYDLAAGLTA
                     PIFNAGRLAAGRDLALAQREELLANYRASIVAALGDVETALNAIDGVDRQRQAQDEAL
                     TQAQAAVRLAESRYRAGAETLLALLDAQRTLYDAQDQAVQLRLARLQASVALYKALGG
                     GWQSVRKTS"
     gene            complement(20614..22575)
                     /locus_tag="VGC69_03440"
     CDS             complement(20614..22575)
                     /locus_tag="VGC69_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEY0294372.1"
                     /translation="MPAEPALIALRDIRKSYGGSDGSPATEVLRGVSLSIAAGEFVAL
                     MGASGSGKSTLMHILGCLDRPTSGQYRFAGQDIAAFTPDELAWLRREAFGFVFQGYHL
                     IRTLDALHNVEVPAVYAGQPVQAREGRARSLLDRLGLAERLDHRPNQLSGGQQQRVSI
                     ARALMNGGHIILADEPTGALDSKSGTEVMALLKELAEASHTVILITHDRNVAEQARRI
                     IEIKDGQVVSDSGPSPASAIASLTPEGFAARMAAGVAHTVSAWADAREAVASAWRTLA
                     VNRVRTLLTLLGIVIGVASVIVMLAIGMGTQAQVIAKMALFGTNRLYVVPGGDNARGL
                     GGTLSEADVDVVRPILNVAAAMPFLNGQVTLRAGHVDTAAKMWAVGSNASRILSWKPV
                     KGLFFTSQDERDLATVLLLGKKVRQRLFGQADAVGQYVLVNNVPFRVIGELAEKGAVT
                     GDADDDDVVLMPYSTGSRRVLGKTDLSWISVLVDDLSKADATQQAIMAQLEAAHRVRD
                     FRVYNAAAAVQAEEDTQRTLTLMLGLIAAISLVVGGIGVMNIMLMSVKERTREIGIRM
                     ATGARQRDIQRQFLSEAVMVSLVGGCAGVVIGLAIGAALIAWDVPAIFSIRGILGAFA
                     CAVVTGLVFGFAPARQAARLDPVVALASE"
     gene            complement(22590..23672)
                     /locus_tag="VGC69_03445"
     CDS             complement(22590..23672)
                     /locus_tag="VGC69_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0294373.1"
                     /translation="MVDTVPITRGNIEVSVTALGTLQPRSYVDVGAQASGEIRRIAVQ
                     PGSPVKKGDLLVEIDPSVQQARVDADRAALDSLRAQLAEQEADRDLARQQVDRQRRMA
                     AEGSTRDEDVQSAQAALRVAEARIANLRAQIAGATSTLQGDQAQLGYTRIYAPMSGTV
                     VTLDAREGQTLNATYATPKILRIADLSSMTVWTQVSEADVRRVKPGMPVYFTTLGDIR
                     RWTGKVRQILPAPPNSPESESSEASKASAAPTAGKVVLYTVLFDVDNADGALMPQMSA
                     QVFFIEAAAQGVVLAPLTAMQAVAGKPGVYAAQVQQADGHVVSREVRTGVQDRLQAEV
                     LSGLTLGDRLVTGNRYVRATDGRFQW"
BASE COUNT         4235 a         8076 c         7413 g         3950 t
ORIGIN      
        1 gcgcacgcgg ttgaacagct cggtcaggcc cggctgcgcg atggcatagc ccacgcgcag
       61 gcccgccagg ccgtaggcct tggagaaggt gcgcgacacc accagattgg gatagcggcg
      121 caccagcgcc acgctgtcga aacgcagtcc cgggtcgagg aactcgttgt aggcctcgtc
      181 caggatcacc gtcacgcgct cgccgtgggc cgcgtgcacc ttggccagga aggcctcgat
      241 ctgcgcgccc gacaggaaag tgccggtggg gttgttcgga ttggccacga acaccaggcg
      301 cgtgtcgtcg gcaatggcgt cgaacatcgc gtccaggtca tggccgtggt ccctggccgg
      361 caccacgatg tgccgtgctc cgcgggcctg cgtggccagg cgatacacca cgaaggaata
      421 ctgcgcatac acggccgagc tgccttgctg cagcagcgtc atggccgcca gttcgagcag
      481 gtcgttggag ccgttgccca acgtcagcca gtccaggggc acgtcgtagc gcgccgacag
      541 cgccgccttc aggtcgaatc cgttggcgtc gggatagcgg gccagcgtgg cggccgcgtc
      601 gccgatcgcc gcgcgcgccg aggccggcat gcccagcggg ttttcgttgg aggccagctt
      661 gatgatgccg gcggggtcca gtccgaattc ccgggccagt tcctcgatgg gcttgccggc
      721 ctggtaggcg ggcagttggc cgatatgggc cggggcttcg aaagattgct tgctcatgat
      781 ctgactctga tgtctcgcgg aatattggaa tgtttgtgcg ccgtcgcgcc gcctacccgg
      841 cccggcctac tgcgccggat acgagcccag cagcttgaaa aacgccactt gccgctgcag
      901 ggccgccagg gcctttgcca ccgccgggtc gtcgcgatgg ccttcgacgt cgatgtagaa
      961 gtaatactcc cactgccccg tgcgggccgg gcgcgattcg aagcgggtca tggacacgcc
     1021 gttgtcggcc agcggcgcca gcatttcgtg catggccccg gcccggttgg gcacggccag
     1081 gatcaggctg gtcttgtcgc ggccgctggc cgccggctgg atatggccga tggccagaaa
     1141 gcgcgtgcgg ttctgcgcgt cgtcctggat gcccgactcg accaccgaca ggccccagct
     1201 cgccgcggcg atctcgcccg cgatggcggc cacgctgccg tcctcggaag ccacgcgcgc
     1261 cgcctcggaa ttgctggcgg ccgccacggt atgcaggtcg ggataatggt gcgccagcca
     1321 gccctggcac tgggccagcg cctgcggatg cgccgagacc gtggtgacgc cgtccatggt
     1381 gcccgactgc gtcatcaggc aatgccggat caccagcgaa cgctcgccca ggagcttcag
     1441 cggcgaggcg agcagcaggt cgaggctgcg gttgaccgcc ccctcggtgg aattctcgac
     1501 gggcaccatg cccaggtcgg cctggccggc ctccaccgcg cggaacacct cgtcgaacga
     1561 gccgcacggc aggcggttga ccgaatggcc gaaatgctcg agggccgcct gctcggagaa
     1621 cgagccgcgc gggcccaggt aggccaccgt catgccgcgc tccaggccgc ggcaggccga
     1681 catgatttcc gtccacacgg cggcaatggc cgcgccgggc aagggacccg gattcccggc
     1741 ctgcaggccg cggatgacct gcgcctcgcg ctcgggccgc agcaccgggc cgccgtcccg
     1801 ggccgcctgc ttggccacgc ccacttcctg ggcggtgcgg gcacgctggt tcagcagttc
     1861 gaggatcctg gcgtcgagct cgtcgatgcg cgcccgcaat gggcccagcc gggcctgcag
     1921 cgacgcgtca tccatggcgg cgctcgaatg cctgcaggta gtcgaccagg gcctgcacgc
     1981 cttcgaacgg catggcgttg tagatcgagg cgcgcatgcc gcccacgctc ttgtggccct
     2041 tgagctgcat caggccggcc tcttcggcgc ccttcaggaa cacctcgttg agcgactcgt
     2101 catgcaacac gaaaggcacg ttcatgcgcg agcgcatcgg cacgtgcacc gggttgcgat
     2161 agaacccggt gccgtccagg tagccgtaca gcagctcggc cttggcgcga ttggccgcct
     2221 cgatggccgc cacgccgccc tgggccttga gccacttgaa caccaggccg gccatgtaga
     2281 tgccgaaggt gggtggcgtg ttgtagcgcg aatgctcggc cgccacattg gcgtagtcga
     2341 aggccgaggg gcagatggac agcgcgtggc cgatcaggtc gcggcgcacg atcaccacgg
     2401 tgacgccggc cgggccgacg tttttctgcg cgccggcgaa caccaggccg gcctgctcga
     2461 tgggcagcgc gcgcgacagg aagtgcgacg aggcgtccac caccagcggc acgtccggcg
     2521 catccagcgc ggccatgtcc ggccagtcca ggttctcgac gccgccgatg gtttcgttgc
     2581 tgcacaggtg caggtaggcg gcgtccttgt tgatcgacca ggtgtcggtc ggcggcaccc
     2641 aggtccaggc ggactgctcg cggccgtcga tcatcgcggc ctggccgctc gaggccacga
     2701 tgcgggcatc gccgtagcgc cccgcttctt tatgcgaacg caccgaccag tggcccgtca
     2761 ccaggaaatc cgccgccggg ctggcgcgcc ggcccagcag gttcatggga acgatggcgt
     2821 tctcgcccag gccgccgccc tgcatgaaga gaatggcgta gtcggccggc agcgccagca
     2881 gctcgcgcag atcgttttcg gcctcgtcgc agatctgcac gaactgcctg ccgcgatggc
     2941 tcatttccat caccgacatg ccgctgccgt gccaatcgag catctcggat gccgcctgct
     3001 gcagaaccgc ctcgggaatg gccgccgggc ctgccgaaaa attccaggga cgcgccattg
     3061 cctctctcct tactccgtgc cttcctgcgc ttgcgcgccg tcgtcctgtg ccggaccttc
     3121 gtctgcattt tcatcttcgt cggcgtcgct ctcgaccacg cggcgcacgc ccgacaaact
     3181 gctgccgtcg tccacgctga tgagcgtcac gccttgcgtg gcgcggccca tttcgcggat
     3241 ctcgctcacg cgggtgcgca ccagcacgcc gcccgtggtg atgagcatga tttcatcggc
     3301 cggattgacc agcaccgccc ccaccacctt gccgttgcgc gacgaggtct ggatggcgat
     3361 catgcccttg gtgccgcggc cgtggcgggt gtactccacg atggacgtgc gcttgccgta
     3421 gccgttctcg gtggccgtga gcacgctctg cgactcgtcg cccgccacca gcagcgaaat
     3481 caccgtctgg ccgtcctcga gcatcatgcc gcgcacgccg cgggcattgc ggcccatggg
     3541 gcgcacgtcg ttttcatcga agcgcaccgc cttgccggaa tcggagaaca gcatcacgtc
     3601 gtgcttgccg tcggtgagat cggcgccgat caggtagtcg ccgtcgtcca ggtcgaccgc
     3661 gatgatgccg gccttgcgcg ggttggagaa gtccgacagc ggggtcttct tgaccgtgcc
     3721 gcgcgaagtc gccatgaaca cgtagtgatc gtcgccgaac accttgaccg gcagcaccac
     3781 ggtgatcttc tcgccgtcgg ccagcgggaa catattgacg atgggccgcc cgcgcgaacc
     3841 gcgcgagccc gacggcactt cccagacctt cagccagtac acccggccgc ggtccgagaa
     3901 gcacagcagg aagtcgtgcg tattggcgat gaagagctgg tcgatccagt cgtcttcctt
     3961 catggccgtg gcctgcttgc cgcgcccgcc gcggcgctgc gcgcggtact cggacagcgg
     4021 ctggctcttg atgtagccgc tgtgcgacat ggtcaccacc atgtccatgg gggtgatcag
     4081 gtcctcggtg tcgatctcgg tggcgttggt ctcgatctcg gaacggcgca gatccttgtc
     4141 cttggcgccc gcggagaact cggacttgat ggcctgcagc tcgtcgctga tgatggccgt
     4201 gatgcgctcg ggccgcgaca ggatgtcgag caggtcggcg atggtttcca tcacttcccg
     4261 gtattctccg atgatcttgt cctgctccag gccggtcagg cgctgcaggc gcatgttcag
     4321 gatttcctgc gcctgggtat cgctcaggcg gtacaggccg tcgtcctgca gcccgtaggc
     4381 cgggtccagg tcgtcgggcc ggtaggccac ccggccgccc ggcgtgtcgc cgccatcggc
     4441 gcgcaccagc atctcgcgca ccagcgacga atcccacgaa cgcgccatca ggtcctgctt
     4501 ggccaccggc ggcgtgggcg ccgcgcggat gatggcgatg aaatcgtcga tattggccag
     4561 cgccaccgcc aggccttcca gcacgtggcc gcgctcgcgc gccttgcgca gctggtacac
     4621 ggtgcggcgg gtgaccacct cgcgccggtg cgagaggaag tactcgacca tctgcttgag
     4681 gttgagcagg cgcggctggc cgtcgaccag cgccaccagg ttcatcccga aggtgtcctg
     4741 cagctgggtg ttcttgtaca ggttgttgag gacgacctcg ggcacctcgc cgcgcttcaa
     4801 ctcgatcacc aggcgcatgc cgtccttgtc ggactcgtcg cggatgtcgg aaatgccctc
     4861 gatcttcttc tcgttgacca gctcggcgat gcgctcctgc agcgacttct tgttgacctg
     4921 gtaggggatc gcgtcgacca cgatggactg gcggttgccc ttttccatgt cctcgaagtg
     4981 ggtcttggcg cgcatcacca cccggccgcg gccggtgcgg tagccctcgc gcacgcccga
     5041 gatgccgtag atgatgcccc ccgtggggaa atccggggcc ggaatgagct cgatcagctc
     5101 gtcgatcgag caatcggggt tgcgcaggca atacaggcag ccgtcaacca cttccgccag
     5161 gttgtgcggc gggatattgg tggccatgcc caccgcgatg cccgagctgc cgttgaccag
     5221 caggttgggc aggcgcgagg gcaagagcag cggctcttgc tcgctgccgt cgtagttggg
     5281 gccgaagtcg acggtttcct ggtcgatgtc ggccagcagt tcatgggcga tcttggccag
     5341 gcggatttcg gtgtagcgca tggccgcggc gttgtcgccg tcgatcgagc cgaagttgcc
     5401 ctggccgtcc accagcatgt agcgcatgga gaaatcctgc gccatgcgga cgatggtgtc
     5461 gtagaccgac tggtcgccgt gggggtggta cttgccgatg acgtcgccca cgatacgggc
     5521 ggatttcttg taggcccggt tccaatcgtt gttgagctcg tgcatggcat acagcacgcg
     5581 ccggtgcacc ggcttcaagc catcccgcac atccggcagg gcccgcccca cgatcacgct
     5641 catggcgtaa tcgaggtagc tgcggcgcat ctcttcttcc agcgataccg gaagcgtctc
     5701 cttggcaaag gaatccatat ttattagcga taagagcgac aaagagtaag ccgggcccgg
     5761 ccagacagat cgccggccgg gaaacagaaa attctatcat tgacccaccg gccaccccag
     5821 gcggcggcgg ttcggccccg tgttccgacc aaaatgacca ttcgctgaac gtcccgcgcc
     5881 ctgcctggtg cccagcccga tgcgtgttgt caaaaaccaa catgaaaaac acgtccaggg
     5941 ctgaaaaggt catatcccgc ggcaatgcga aaggccgaaa gcgccaaacc tcactaaatg
     6001 gcttaagatt gtttccagca cgcagccagt gtggcgacct tagaattcgt tctcggcctt
     6061 gctatactgg ccccgttttc ctgatctgcg gcgggggacg ctgcgagtca tctatccagc
     6121 ttcaagctca acgaggagaa acatgaacaa accctccaaa ttcgctctgg cgctcgcctt
     6181 tgccgccgtt acggcctcgg gcatggcttc cgcccaaacc ggcacccgta tcgacaattg
     6241 gcgcgacaac tttggcgatg tgtggaagaa cggcacccgc gaatattgct ggcgcgacaa
     6301 cttctggacg cctgctaccg gcatccaggg ttgcgacggc gtcgcctccg ccccggcccc
     6361 cgccaagggc gctccggttg cttcgaaggt cgtcttcaat gccgacgcct tcttcgactt
     6421 cgacaaggct acgctgaagc ccgaaggtcg ccagctgctg gaccaggtcg ctgcccaaat
     6481 cggcacgatc aaccttgaaa ccatcatcgc cgtcggccac accgactcga tcggtacggt
     6541 tgcctacaac cagaagctgt ccgagcgccg cgctgctgcc gtcaagacct acctggtcag
     6601 caagggcatc gacgccaacc gcatctacac cgaaggcaag ggcaagacgc aacccgtggc
     6661 ggacaacaag accgccgctg gccgcgccaa gaaccgccgc gtggaaatcg aaatcgtcgg
     6721 cagccgcaag taatttctgc cgtagcagcg gttggcctgc caaccgctac aataaagagc
     6781 ctcgccatgc gaggctcttt ttttgccttc acgatatgag caccgacacc tccgcccgca
     6841 acggcacgaa cggcaacgta aacgccgacc aagccgaact cgacaaattc agcgccctgg
     6901 cctcccgctg gtgggacccg gaaagcgagt tcaagcccct gcacgccatc aaccccctgc
     6961 gcctggactg gatccgcgaa caggtaagca gcctcgccgg cctgcgcgtg ctcgacgtgg
     7021 gctgcggcgg cggcatcctc gcggagagca tggcccgcgc cggcgccgac gtcaccggca
     7081 tcgacctggc cgagaaatcc ctcaaggtgg ccaagctgca cggcctcgaa tccggggtca
     7141 aggtcgacta tcgcaacatc ccggtcgagc agctggccac ggaacagccc ggcgctttcg
     7201 acgtcgtcac ctgcatggaa atgctcgaac acgtccccga cccggcctcc atcgtgcgcg
     7261 cctgcgccac gctggccaag cccggcggct gggtgttctt ctccacgctc aaccgcaacc
     7321 ccaagtcctt cctgttcgcc atcgtcggcg ccgaatacct gctcaagctg ctgcccaagg
     7381 gcacccacag ctacgccagc ttcatcaagc ccagcgagct ggccgccttc acgcgccagg
     7441 ccggtctggc cgccgtcgag ctcaagggca tggaatacaa cccggtcacc caggtgtatt
     7501 cgctcaccgg cgacacctcg gtgaactacc tggtcgccac ccgaaaacaa taagctggaa
     7561 gccccaccca tgagcgcact ggtcttgttc gacttcgacg gcaccctggc cgacaccgcc
     7621 cccgacctgg ccgccgccgc caacgcccag cgcgcccggc gcggcatggc gcccctgccc
     7681 gtcgacgtcc tgcggcccgt tgcgtcgcaa ggcgcgcgcg gcatgctgcg cgtggcgctc
     7741 gacatgctgc ccagccaccc cgaattcgag gcagcccgcc agcagttcct cgaccactac
     7801 gcggccaatt ccaccgtgca cagccggctc tttcccggca tcgccgaact gctcgagcgc
     7861 attcgagccc agggccacgc ctggggcatc gtcaccaaca aggtcgccca cctcacccgc
     7921 cccatcgtcg aacacctggg cctggcgcgg cactgcgcca ccctggtctg cggcgacacc
     7981 acgcccaccc ccaagcccca ccctgctccg ctgctgcatg cggcacagga agcgggcttt
     8041 ggcgtcgagc attgcctcta cgtgggcgac gacctgcgcg acgtgcaggc cgcccatgcc
     8101 gccggcatgc cggccgtcgc cgcggcgtat ggctacctgg gcgacggcgg cgcggtggac
     8161 acctggcagg ccgacgccca ggccaagaat gtggccgaac tttggcccgc aatcgccgca
     8221 ttgctgccaa aataacggat atgcgccgcg ccgagggcaa tgtcccaggc gcggcgcaac
     8281 agcgctttcc ccctacgcag tatcagtcga ctcgtcgata ttgagggacc gcccctaaaa
     8341 tatagttaaa agcaatcgat cgaatttaat taataaattt gatctaagcc tccagactcc
     8401 ctactattac tgcataccgt cttttcaccc cagcacagag gaaactgcaa tgtctctagt
     8461 caacacccaa atcaagccct tcaaagccca agccttccgt gaaggcaagt tcatcgaagt
     8521 gaccgaagcc agcctgaagg gcaagtgggc cgtggtggtg ttctaccccg ccgacttcac
     8581 ctttgtgtgc cccactgagt tggaagacct ggccgacaac tacgccgaat tccagaaact
     8641 gggcgtcgaa gtctactcgg tgtccaccga cacgcacttc tcgcacaagg cctggcacga
     8701 cacctcggcc gccatcggca agatcaagta cacgatgatc ggcgacccca cctggcaact
     8761 ggccacgaac ttcgacgtgc tgcgtgaagg ccaaggcctg gcagaccgcg gcaccttcat
     8821 catcgacccg accggcgtga tccaggccgt ggaagtcacc gccgaaggca tcggccgcga
     8881 cgccaaggaa ctgctgcgca aggtcaaggc cgccctgtac gtggccgccc acccgggcga
     8941 agtctgcccc gccaagtggc aagaaggcga gaagaccctg gctccttcgc tggacctggt
     9001 cggcaagatc taagcagcaa tcgctagtcc catgcccggg acgcatgtcc cgggcagcac
     9061 caaaagttca cgaatcaccg caataacagg aaatcgatca tgctggacgc aaccctcaag
     9121 tcccaattgc aaacctatat gcaaatgatc tcgcagccca tcgagatcgt ggcgtctgtc
     9181 gacgacagcg agaagtcgcg cgagctgacc gaactcctgc acgaaatcgc cgccatgtcg
     9241 ccgaacatct cgtacaccga gcgccgcgac gacaacgagc gcaagccctc tttcagcatc
     9301 tcccgtcccg gccaggatct cggcatccgc tttgccgcca tccccctggg ccacgaattc
     9361 acgtcgctgg tgctcgccct gctgcaagcc ggcggccacc cgctcaagct cgatgccgac
     9421 gtcatcgaac agatcaagaa cctcgatggc gactacacct tcgagaccta tgtatcgctg
     9481 tcctgccaga actgccccga agtggtccag tcgctgaacg tgatgtcgct cctcaacccg
     9541 cgcatccgcc aagtgaccat cgacggcgcc ctgttccagg acgaggtcga gacgcgccag
     9601 atcatggccg tgccgaccat ctacctcaac ggcgagaact tcggccaggg ccgcatgacc
     9661 gtggccgact tcctcgccaa gctcgacgcc aacgccagca agcgcgacgc cgacaagctc
     9721 aacgcccgca agccttttga cgtgctgatc gtcggcggcg gccccgccgg cgccgcggcg
     9781 gccgtatacg cggcccgcaa gggcgtgcgc accggcgtgg tggccgagcg cttcggcggc
     9841 caggtgctcg acaccctggc catcgagaac ttcatctccg tcacggaaac cgaaggcccc
     9901 cgcttcgccg cggcgctcga agagcacgtc aaggcctatg acgtcgacat catgaacacc
     9961 cagcgcgccg aagccctggt gcccggcgcc gactacatcg aagtcaagct ggccaatggc
    10021 gcctcgctca agagccgctc ggtgatcctc tccaccggcg cccgctggcg cgaagtgaac
    10081 gtgccgggcg aacgcgaata ccgcaaccac ggcgtggcct actgcccgca ctgcgacggc
    10141 ccgctcttca agggcaagcg ggtctcggtc atcggcggcg gcaactccgg cgtggaagcc
    10201 gccatcgacc tggccggcct gaccgagcat gtcaccctgc tcgagttcgg cgatgcgctg
    10261 cgcgccgacg aggtgctgca gcgcaagctg cgcagcctgc ccaacgtcac ggtgctgacg
    10321 caggcgcaga ccaccgagat caccggcgac ggcaacaagg tcaatggcat cacctacaag
    10381 gaccgccaaa cgggcgagac gcatcacatc gagctggaag gcgtgttcgt gcagatcggc
    10441 ctggtgccca ataccgaatg gctcaagggc acgctggcgc tctccaagtt cggcgagatc
    10501 gagatcgacc cgcgatgcca gacttcgctg cccggcgtgt tcgccgccgg cgacgtcacc
    10561 accgtgccgt acaagcagat catcatcgct actggcgaag gcgccaaggc tgcgctgagc
    10621 gccttcgatc acctgatccg cacgccggtg ccggagcagg tcgagcagca ggaagccgtg
    10681 gccgcctgac aaggcgccgc ccggcgctgc cggcaacgcc gatgcaatgc ccgcagggtc
    10741 cgccctgcgg gcattttgct ttccgcaagg gcgcgtcagg ccttggccgc ggcgtcgcgg
    10801 gccattctca cgaaggcctg cgccgcgggc ggcagcaagg cgaacgaacg gtacaggaag
    10861 ccgacctgac gcatgactgc cggcttgacc agcggaacga cggccaggcc ttcctcattg
    10921 cacatccgaa cggcctgctc tggcaggacg gccgcgccca atcccgagcg cgccataccg
    10981 acggcggtcg ccaccaggct gacctcggcg tacggacgca ccgacaggcc attggcggaa
    11041 aaggcgctgt ccatcaattg cctgacgtgg ctgtggtagc tcatcaccac cagcggctcc
    11101 gcgccaagtt cgcgccagcc cagcgtcttg cggtgggcca atcgatgttc cgccggcagc
    11161 actgccacca tgcggtcctg cgcctgcttc tcgaaggtga tatcgctgtc ctctgacgag
    11221 ggagcgccca tcgcaaggtc gatctctccc cggcgcagca tctggatcat gtcgcgcgtc
    11281 tcggtcatgg cgtctttcac tgagatcctc acgtgcgggt acatctggga gaaccgcttt
    11341 atcagcctgg gcaaggcgat cgccccagcg gaaggaatgc agccgatggc taggcttccg
    11401 ccgcgtggca acgtcgcatc gcgcaggccg tccaccgtca attcgagctc gccgatgagc
    11461 cgctggatgg tcggcaggaa ctcgcgcccc gacggcgaca gacgcacgga acgcgtgcgc
    11521 cgctcaagca actggatgcc cagctggctt tccagcttct ggatcatgag tgtcagcgcg
    11581 ggctgcgtca cgctcatggc ctcggcagcg cgggaaaaac ttgccgagcg agccacggcg
    11641 atgaacgcct gcaattggcg cagcgtgatg ttcatttgcc tttattcatt tatttaatta
    11701 ataaatttat tataagaata aatttttaat attttaggct atcccgtaga ctgcccgcac
    11761 aatttcaagg agacacgatg tccgggcacg acaacacgcc ttcgacgggc acttgggcac
    11821 gccgcgacct gagcggcttc ctggccgccg acgccatggc cgctgttctg aaggcggcgg
    11881 aagttccttt catctttggg caaagctgcc caaccgccct gttcctggcc gccgccaagc
    11941 tgggcatcgg ccagatcggc taccgcacgg aaaacgcggg tgtcgccatg gccgacggct
    12001 acgcccgggc cagcggcaag gtcggcgtga tcacggcaca gaacggaccc gcggcgggtc
    12061 tgctggtggc ggggctgtgc gaagcgcaga aggcatccgt gcccatcgtc gccatcgtcc
    12121 aggacgtgcc tcgcgccaac accgacaaga atgcgttcca ggaaatcgac cacgaacaga
    12181 tattccgcgg ctgcgccaaa tgggtcagga aagtggtgga tcccgcgcgc gccggcgagt
    12241 acatgatgcg ggccatcatg gccgccacgg cgggccggcc cggcccggcc gtccttctgg
    12301 tgccgttcga cgtgctcctg gagcaagtcg ctccggtcac gcccgaccgt cccctcgccg
    12361 acggccccct gcccgcgggc ttcggccact atcccgtgga tcggcacgcg cccgcgccgg
    12421 aactgatgca gctcgccgcg caatggatcg ccgacggcga acggccgctg atcgtggcag
    12481 gcggcggcgt gcacacggcc caagccgccg ccacgctggc caagctgcag agcctctggc
    12541 aggtgcccgt cgcgaccacc aacatgggca agggcgccgt cgacgagacc gacccgctgt
    12601 ccatcggcgt gattggcaat tgcatgggaa cgcgcagccc cttcaagcat cagcgtgatt
    12661 acgtgcagcg ggccgacgtg gtgctcttcg tcggcacccg caccaacgaa aacggcaccg
    12721 cctcgtggag cctgttcgac cgggacgcga aattcatcca gatcgatgtc gacccggaag
    12781 aactaggccg caactatcgc ggactcaggc tgctcggcga tgcggacacg accctgcagg
    12841 cgctgctagg ggcgacgccg cccgccctgg ccgagcgggg ccatcgcaac gcgccggcga
    12901 tacgccaggc catttcccgg gctcgcgcgc tgtcggcggc ggagctggcg ccattcgaag
    12961 acagcgcgca gacgccgcta cgcccggagc gcctgttcaa ggaactgcgc gcctgcgcgc
    13021 cggacgcgct gtgggtcagc gacgccagct attcgtcgct gtggatattg caatacctgc
    13081 aggcgcgccg cagcggcgac cgctttctca cgccgcgcgg catcgccggc ctgggctggg
    13141 gttatccgat ggcgctgggg gcccgcctgg cgcagccggc caggccggta atcgccgtcg
    13201 ttggcgacgg cggcttcagc cactgctggg cggaaatcga gaccgcgcga cgccacggca
    13261 tccaggccgt cgtgatcgtc ctcaacaacg gcgtgctggg ctttcaacgc gacgctgaac
    13321 aaagccgctt cggcgtacac accgaaatct gccacttcgg gcccatagac cacgtggcga
    13381 tcgcccgcgg ctgcggcggc gacggcatcc gcgtcacgca ggccgaagag atcgccccgg
    13441 ccctggcacg ggcgctggcc gccacggtgc ccatggtcat cgacgtggac acggcaccgg
    13501 aagccttccc acccatccag gcgttcgaat cgctcaatgc ggacgtagcc gcgcaggcat
    13561 gacaccgata tgagcggact ttcccgacaa cccagcgaac aacgcggcaa gaccggcctg
    13621 gtcacgggcg cggcgggcgg catcggcctg cgcacggccg aacgcttggc gctggccggg
    13681 gcaacggtgc tgatggtgga ccagaaccaa ccggcgctgc agcagcgcgc gcaggcccta
    13741 gccaccctcg gctaccgcgt gcaggccctg gcgctcgacg tggccgacgt ccatgcagcg
    13801 cagcgcgtcg agcaggcgct cgccgagctg ggcacggacg ccgtggacta cctggtcaat
    13861 tgcgccggca tttcacccaa ggcggacggc cgccgcgtgc cagcctggca ggtcgatccc
    13921 gccgaattcc agcgcgtcat cgcgatcaat ctctgcggct acttcaacat ggcgcgcgcc
    13981 gtggcaccag gcatgatcca gacccgccgg ggcgcaatcg tcaacatcgg ctcgctggcg
    14041 ggcctgcgct attcatccat cgccggcgtg gcctacgcca cgtccaaagc gggcgtcacc
    14101 ggcctcaccc aacaactggc cggcgaactc gccccgttca acgtgcgcgt caatgccgtg
    14161 gcgccaggac ggatcgaatc gcccatgagc gccatggcgg ccgcgggcat caacgatgcc
    14221 attctcgccg gcaccccgct gggcaggctg ggcacgccgg acgacatcgt ggattcggtc
    14281 tttttcctgc tttccgaaca ggccggcttc atcacgggcc agaccttggc cgtcacaggc
    14341 ggacgaggtt tatagcgtca ttatttcaac cgaggagaca caaaaaaatg cgaaacaaaa
    14401 cccttgccgg cctcctgacc ggcctcttcc tggcactggg cgcaacgcct gtgctggccg
    14461 gctaccccga cgagcccctg cgcctggtcg tgccctaccc gcccgcgggc attaccgaca
    14521 tcgccgcacg cgccctggcc aagggcatga gcgcagacct caagcaaaac atcattgtcg
    14581 agaaccgcgc aggcgcgggc ggctcgatcg gcacggcctg ggcggcgcac gccgcacccg
    14641 acggctacac catcctgatg ggcaccagcg ccacccacgg caccaacccg accacgctgg
    14701 cctcgctgaa ctacgacgcc gtcaaggatt tcgccgccgt atcgctggtc gcctcggcgc
    14761 cgctggtggt cgaggtgaac ccctccttgc cggtgcacaa catcagggag ttcatcgact
    14821 acctcaagaa gcaccctgac tccgtgttct tcgcttccac cggcacgggc ggctcggtgc
    14881 acctgagcgt cgaatacttc aagctcaaga ccggcaccag catgcagcac gtcccctaca
    14941 agggcagcgc gccggcgctg tcggatctcg tgggcgggca ggtgcaggtc atgttcgaca
    15001 acattccttc ctcggcgccg ctgatcaagg ggggcaagct gcgcgcgctc gcggtgacga
    15061 gcacgacccg catagcggat ctgcccgatg tgcccacggt atccgagtcc ggcgtgccgg
    15121 gtttcgagac cggctcctgg gtcggcatct acgcgccggc aggcacgccg ccggccgtcg
    15181 tggagcgcct caacaaggcc gtcaacgcgg cgctgcaaga ccccgccgtg cggaccgtct
    15241 tcgccaactc cgggctgatc ccgaaagggg acagcagcgc cgacttcgcc gccttcacca
    15301 agcgcgagat cgatacctgg gccgacgtgg cgcggcagat ccactacgtg cctcaatagg
    15361 gaaacagcgc cgcgggatcc agccccaccg aaccggccgc ctcccgcaac tgccccagcg
    15421 tgccgtcgtc gagcgcaatg ccctcgcggc tgcgccgcgc ccggctttcc cgctcggcat
    15481 cgcccggcgc cttgacggct tgccccggta tggcggcctc cttcacccat gccacgaagg
    15541 ggtcgatttc atccatcaac tgccgtccgg cccccagcgc cgccggatcg atgacgatcg
    15601 agagcaggcc attgacgatc gcagcgccct gcgggccatg ctgctcggtc ggcccgccga
    15661 tcagcgaacc cgccagcagc gagcagatca gcgccaggcc ggcgcccttg tgctcgccga
    15721 acggcaactg ggcgcccttg ggctccgtaa acaggaccga cgggtcgtcg gtttgccggc
    15781 cctcggcgtc caccatcgcc gagccgcccg ccacgcgctc gcccttgagc cacgccacgc
    15841 gcgccttgcc atgcgccacg cggctggtgg cgaaatcgag gatgacgtga tccccggtcg
    15901 gagacggcat tccgatgcag accggattgg tcccgaaacg cgcctccagc ccgttccagg
    15961 gcaccaccga agggtgggac gaaacgttga cgaaatgcag cgacaccagc cccgctgccg
    16021 caagctgctc cgcataggcg ccgatgcgcc ccaggtgatg cacattgcgc aagcccagca
    16081 tggcgaggcc ctggccacgc gcccgggcct cggccagccg catggcatgg ctgcccacca
    16141 cctggccgta gccgcgccgg ccatccatga ccaggatcgc gccctggtcg accgccacct
    16201 ccgccgtatc ggcattgggc tgcaggccgc cctcggcaat ggcctggaca tagcgcggcg
    16261 ccatgcccac gccgtgcgaa tcatggcctt tcaggttggc ctcgaccagg ttgtccgcga
    16321 cctgccgcgc gatctccgcg cgcgttccgg ccgactcgaa cacgcgggcg accgactggg
    16381 ccaatggaag gggaagcatc tgcaacatcg cgggaaacac cttgtgtgaa tgggttgggt
    16441 tgggatcctg ttttaacaaa cccgcgcctt cgcccctagt gatgcatacc cgggcatcgt
    16501 ttgcgccttg ccaggcggcc gccgggctga cggcggcatt cacacgtata ataggaagtc
    16561 tcggggccga tccggattcg acgtggatca agaaacagct cagggcatgc cgagcaccag
    16621 tacgctcgta aaaccactgg aaacgcaata aacgccaacg acgagcgttt cgctctcgcc
    16681 gcgtaagcgg tgagccgctg caccgatctg tccttgggtc gggcggagaa ggggcaaccc
    16741 ttctaaggat tcctgcctag ggcctatatc ccccgaagca gttttatcct gaaccgcagc
    16801 agcgtcattc acaaggaatc ggtttgcgct ggggtcacac ggcgtccgct taaattacgt
    16861 gaatcgccct ggtccggcct gtcgtccggc taagtccagg gttaaatcca aataggccga
    16921 ctaagcatgt agaactgatt gtggatggtt tgcggacgcg ggttcaattc ccgccggctc
    16981 caccaaatac ctgtccagca tagttcgctg gcatttgtga cgaaacccat aaagtggcca
    17041 ggtggccact ttatgggttt ttttctgatg ggccacatta tgggttcatg gggaatgcct
    17101 agctgcgatg ctccttggca taatccagcc atgccacatc agagacaaga aatggacaac
    17161 aagaatggaa cgactatctg gttgtttggt cgtggcctgt catgcaactg cgggctgacc
    17221 tggacggtac ctgagagttg gagaagcctg ccacgcgacg agcagatcga acgcatcaaa
    17281 ccagcgatac gcaccgagat gaactctccc aatgtggaca tctcctccat ccggcgttta
    17341 cttgatgatc ttgccgcgaa aaccccagat ggtcagaagc atcgattcgg tacgacaaac
    17401 tgggactatc tgctacaacg tgagattctc gtacggtcac atgatgcgtg ggatggtatt
    17461 cagcccgtat ggctggcgga atcacacgtt tcacatatca acggcaccgc cgaggagcta
    17521 gaaaataacg cacatcgaag cccctttttg cttgaggagg acgcctacga taaacgcacg
    17581 aagtccgttg aggccgacaa tttttacaac ttcatgatcg ggggctggcg gttcgtagtc
    17641 gttggcatgt catttgagtg tgagatcgat cggtttctcc tttactccct tgggagggtc
    17701 caggacgacc ttcctattgg tgagtcaact tggctagtgg ttaacagaaa ctatgaagcg
    17761 ttggaaaaaa cccgtacagc gattgcagac catttgccac gcgcaaaaat cgtcgccgtc
    17821 caaagtagct tttccgattg gcagaataac gggtactctg gactatggga catgccatga
    17881 caggatcgcc cttaaagagt acgactccac cctgaccagc attgcatcta ggtgaagctc
    17941 ggaatagccg accccaccca ccaggatact gcgctctagt tgatccgggc cttgaccggt
    18001 cgaaccgatg aaatcgtgtc ggacatcctg caagatgccg ggatgaggat ggggctgggg
    18061 atgccgccgc aagaagaaag acagccggct tggcctatct gaaagtcaca gcttaattaa
    18121 gcgtagtttc atggctcctg aagcggcaac cgtgcgctat acccttttgc tgccacacca
    18181 agcagttgct gtttcttggt tgagcgacac cgtacccagg accgccctca ttgaggcttc
    18241 ggcgcatgat acgaggtcgt gatgccaccg ttcagcgctc aactgctggt gggatagccg
    18301 cctgcgccga cgacggaaac caccactggg acgcagggga cactgggccc agggggatta
    18361 gacgtgagag cccagcgtac tggcgaagct agaggtacgt gtcgtaagtc tggcggacgt
    18421 tagcagctca aattcgcggc tggggaaaat ccctggccgg agctgccaac gcgaaggaca
    18481 tcccgcgttt ctcgctcagg gaggtgtcgg attgtccgaa aatcggataa tccgaggtaa
    18541 accagcggag tgatacgtcg gcaagcccga tatctggtag gccaccttgc tgcaggatca
    18601 ggagcaaggg ccgacggggg caaggacggc tcttgtctca cccagaggag tcggcgtgtt
    18661 cgcaggccag ccgttccgca tcgcaatctg gcagcgaggg ttttatccgt ctttcccgcc
    18721 ccaaggtgcg acgatctact ttgccagcat tggcgccgcg tctcctgggt gagcgatggc
    18781 ggcgtgcgcg gcaaggccta ccctcgaacg cactggacct gtacggtgat aaccgactca
    18841 gtgagcagca agcttcgatc ccgcagtgcc cttgcaacat gttcgcgcaa ggtctcatcg
    18901 acgaattgaa atgtatcgcc tgtcgtgtgg cattcgatct tatgcagcgc gggattgctg
    18961 ccggcggggc ggatgcggta gaaccgcttg ccgttgtcgt cggtctccct caccagcaga
    19021 ccgtgctgcg ccaactcgtt cagaatccga tagaggtttc caatgctgat ctgctcgccg
    19081 tgctgtatgc actctcgata caagccctcc gaagacgtcc agcgctccgg ggcacgttgc
    19141 atggcccgca gtacggccat tcgagaggcc gtggccttca gtttcacggc caagagttgg
    19201 gtcaggatcg acgcatcaga aataggttca agcaccttca tgatgtcttc ctcacggatt
    19261 gccagccacc acccagcgcc ttgtagagcg caaccgaagc ctgtagacgg gccagccgca
    19321 actgcacggc ctggtcctgc gcgtcgtaga gcgtgcgctg ggcgtcgagc agcgccagca
    19381 gcgtctcggc gccggcccgg tagcgggatt cggccagtcg caccgcagcc tgcgcctgag
    19441 tcaaggcttc gtcctgcgcc tggcgctggc ggtccacacc gtcgatggcg ttgagcgcgg
    19501 tttccacatc gcccagggcg gcaacgatgc tggcgcgata gttcgccagc agttcttccc
    19561 gctgggccaa cgccaggtcg cggccggcgg cgagccgtcc ggcattgaag atcggcgccg
    19621 tgaggccggc ggctaggtcg tagatcgggt ggtcgaacag cccacgcagc cggtcgttgc
    19681 ccacgccagc cgaagcggtc agcgtgacgc tcggcaacat ggcggcacgt gccaccgtca
    19741 cgtcggcgtc ggccgtagca agctgccgtt cggcctgggc tacgtcgggc cgatgcgaaa
    19801 ataaattggc cggtacaccg gcatcggcag acggcgtttg cacgccatcg agccccgagc
    19861 cggcaacgac cacctcttgc agcggtcggc cgatcagcgt ggccaaggcg atccggctgt
    19921 cgtgcgcttg ctgcgcgcgc agggccacgg cctgccgaag ctgggccacc agcgtgcgct
    19981 gctgcgccag gtccagggcg gtggccgcac cggcacggta ctggctctcg acgaaggtca
    20041 gaatgcgctc gcctgccgcc aggttctggc gagcgatggc cacgcgttcg cgcagcgcca
    20101 gcgttcgcag gtaggtatcg gccacgcctg ccgtcagcgt cagcatcacg gcgtcgcttg
    20161 cgtagctggt ggccaccagc acatcaactg ccgcttggcg ggcggcgcgg ttggctcccc
    20221 atacgtccaa ctcgtagcta gcggacagcc cggcggtata agcggtcagc ccgggatagc
    20281 cgaccgtcct gcttcgttct accgaagccg taccgtcgac ctcgggcaac agcgcggcgc
    20341 cggccatccg ggccagcgca tcagcctggc gcacgcgggc ggcggccgcc gccacgtcaa
    20401 agctgccggc gcgggccgtc tcgaccaggc cggccatctc ggcgctgccg aaccctcgcc
    20461 accagtcggc atcgaccggt tcgcccggca cagaggtcgc ctgccagtta cccggcaagt
    20521 ccaggccggc gtcggtatgg gcgatgggca ggccacaacc gcccagggcc aaagcccagg
    20581 ctagcgcagc gagacggaat actcggtgcc ccttcattcg cttgccaacg ccacgacggg
    20641 gtcgagccgc gcggcctggc gcgccggcgc gaagccgaac accaggcccg tgaccaccgc
    20701 gcaggcaaag gcgcccagga tgccgcggat ggagaagatg gccggcacat cccaggcgat
    20761 caatgctgct ccgatagcaa ggccgataac cacccctgcg cagccaccca ccagcgacac
    20821 catgacggcc tcggaaagga actggcgctg gatgtcgcgc tggcgagcgc cggtcgccat
    20881 gcggatgcca atctcccggg tacgctcctt gaccgacatc agcatgatgt tcatgacgcc
    20941 gatgcccccc acaaccaagg agatcgccgc gatcagcccc agcatcaggg tcagcgtgcg
    21001 ctgcgtgtct tcctccgcct ggacggcggc ggccgcgttg taaacgcgga agtctcgaac
    21061 acggtgcgcg gcttccagtt gcgccatgat ggcttgctgt gtggcatcgg ccttcgataa
    21121 atcgtccacc agcaccgaaa tccacgaaag atcggtcttg cccagcaccc gccggctacc
    21181 agtggagtac ggcatcagca ccacatcgtc gtcgtccgca tccccggtga cggcgccttt
    21241 ctcggccaac tcgccgatca cacgaaaggg tacgttgttg accaagacgt actggcccac
    21301 ggcatcggcc tggccgaaca ggcgctggcg gaccttcttg cccaacagca gcaccgtggc
    21361 caggtcgcgc tcgtcctggg aggtgaagaa caaccctttc acgggtttcc agctcaggat
    21421 gcgcgacgcg ttgctgccga cggcccacat tttggccgcc gtatcgacat ggcctgcccg
    21481 cagcgtcacc tgcccgttca agaacggcat ggccgctgcg acattgagta tgggccgcac
    21541 cacgtccacg tcggcctccg agagcgtgcc gcccaagccg cgggcgttgt ccccgccggg
    21601 caccacatac aagcggttgg tgccgaacag cgccatcttg gcgatcacct gggcctgcgt
    21661 acccatgccg atggccagca tcacaatgac cgaggccacg ccgatgacga tgcccagtaa
    21721 ggtcagcaac gtgcgtactc ggttgacggc cagggtgcgc caggctgatg ccacggcctc
    21781 cctcgcgtcg gcccaggcgc tgacggtatg ggcaacgccc gccgccatgc gggcggcaaa
    21841 gccctctggt gtaagcgatg cgatggcgga cgccggcgac ggcccggagt cggaaacaac
    21901 ctggccgtcc ttgatttcga tgatgcggcg ggcctgctcg gccacattgc ggtcgtgggt
    21961 gatgaggatg acggtatggc tggcctcggc cagttccttg agcagcgcca tgacctcggt
    22021 gccactcttg ctgtccaagg ccccggtcgg ctcgtcggcc aggatgatgt ggccgccgtt
    22081 catgagtgcc cgagcgatgg agacccgctg ctgctgcccg cccgagagct gattcggccg
    22141 gtggtccaag cgctcggcca ggccgaggcg gtccagcaaa gaacgggcgc gaccctcccg
    22201 agcctgcacc ggctggcccg cgtagacggc cggcacctcg acattgtgta gcgcgtcgag
    22261 cgtgcggatc aggtgatagc cctggaagac aaagccgaat gcttcgcgcc gcagccaggc
    22321 caattcgtct ggggtaaatg ccgcaatgtc ctgtccggcg aaccggtatt gcccggacgt
    22381 cggccgatcc aggcaaccca ggatgtgcat gagcgtggat ttgcctgacc ctgaagcccc
    22441 catgagcgca acgaattccc cggcggcaat cgacaacgac acaccgcgca gcacttcggt
    22501 tgccggacta ccatcggacc cgccgtagga cttgcggatg tcccgcagcg cgatgagtgc
    22561 gggttcggcc ggcattgccg atcctgcggc taccactgga acctcccgtc cgtggcccgg
    22621 acataccggt tacccgtcac gagccggtcg ccaagggtca ggcccgacag cacctcggcc
    22681 tggagccggt cctgcacgcc cgtccggact tcccggctga ccacgtggcc gtcggcctgc
    22741 tgtacctggg cggcataaac gcccggtttg cccgccaccg cctgcatggc cgtgagcggc
    22801 gccaacacca caccctgcgc ggcggcctcg atgaagaaca cctgagcgga catctgcggc
    22861 atgagcgcgc cgtcggcgtt gtccacgtcg aacagcaccg tgtagagcac gaccttgccc
    22921 gctgtcggtg ccgctgatgc cttggatgcc tccgacgatt ccgattcggg cgagttgggt
    22981 ggcgcaggca atatctgacg gaccttgccc gtccagcgac gaatgtcgcc cagggtagtg
    23041 aagtacaccg gcatgcccgg cttgacccgc cgcacgtcgg cttccgatac ttgagtccaa
    23101 acggtcatgc tggacaggtc ggcgatgcgc aggatcttgg gtgtggcgta ggtggcgttc
    23161 agcgtctggc cctcgcgggc atcgagcgtg accaccgtgc ccgacatggg cgcatagatg
    23221 cgggtgtagc cgagctgggc ctggtcaccc tgtagggtgg acgtggcccc ggcgatctgc
    23281 gcgcgcaggt tggcgatgcg ggcctcggcc acgcgcagcg ccgcctgggc cgattgcacg
    23341 tcctcatcgc gggtcgaacc ctcggccgcc atgcggcgct ggcggtcgac ctgctggcgg
    23401 gctaggtcgc ggtcggcttc ctgctcggca agctgggcac gcaggctgtc cagcgccgcc
    23461 cggtcggcgt cgaccctagc ctgctgcacc gaagggtcga tctccaccag caggtcgcct
    23521 ttcttgaccg gactgcccgg ttgcacggca atccggcgga tctcgccgga cgcctgggca
    23581 cccacgtcta cgtagctgcg cggttgtaag gtgcccagcg ccgtcacgct tacttcgata
    23641 ttgccacggg tgatgggtac ggtatcgacg atct
//
LOCUS       DASWPO010000056         4723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1171828, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000056 DASWPO010000000
VERSION     DASWPO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171828"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /gene="bglX"
                     /locus_tag="VGC69_03450"
     CDS             <1..1360
                     /gene="bglX"
                     /locus_tag="VGC69_03450"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HEY0294374.1"
                     /translation="GTDLSMSDTIYGGNLEPLLKRGKIRMADIDRACRDVLRVKYDLG
                     LFKDAYRDIGVAADDPPDTYAESRLHRPEARDVARESLVLLKNQNQALPLKKQGTIAL
                     IGPLADDQVDVMGNWAGAGRIPQTIALLQGVKNAVGDKARVLYAKGSNMTDDPDLVQY
                     LNQYNHDVQVDPRSEDDMVKEAVEAARQADVVVAAVGESQGMAHEASSRSDITLPPVQ
                     VRLLEALKATGKPLVIVLMNGRPLALVREAGMADALLETWFAGTEGGNAIADVLFGDA
                     NPSGKLPMTFPRAVGQVPLYYNHLPTGRPFDPVHPDKYTSRYFDITNGPLFPFGYGLS
                     YTTFDVSDVSLSAPAMKAGGTMQARVTVTNTGDREGATVVQLYLQQPVASVSRPVREL
                     KGFRRISLKPGESRVVSFDITDDDLAFYNAGMKRVVEPGKFNVYVGLDSDADKLASFN
                     RQ"
     gene            complement(1603..2514)
                     /locus_tag="VGC69_03455"
                     /pseudo
     CDS             complement(1603..2514)
                     /locus_tag="VGC69_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
     gene            complement(2511..4181)
                     /locus_tag="VGC69_03460"
     CDS             complement(2511..4181)
                     /locus_tag="VGC69_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0294375.1"
                     /translation="MIEDANRTPAGATLHTDLCIVGAGAAGIAMALALQDSGLDVILL
                     ESGADQPDDATQALYEGTVADARLHSPPDHYRVRRFGGSTTLWGGRCIPLDPIDFEPR
                     SYIAHSGWPIGPDDLLPYYPRANLLCEAGDFAYTVETAFTHPVRPMIGGFDSDAFSTN
                     TLERFSCPTDFGSRYRRRLERGPARVLFNANLVGLSTAADGRLAGPATVRTLAGNHFT
                     VQARAYVLAAGGLEVARLLLASPGASGRGLGNARDVVGRYYMSHIAGTMGTADLSRAS
                     SVWHGYEISDEGIYCRRRLALRPQAQRALQAGNFIARLHHPRIPDPGHGSGILSALYL
                     ARPIVPYEYAKRLYGDEPGRGATLAHLRNVVLDAPGTAAFLWNWLRRRTLAERKFPSI
                     VMRPKNLRFSLDFHAEQEPNPSSRVKLGQGVDALGMPRIDVDWRYTERDVQTVRAALA
                     ALAQAFRQTGVGSFDYEPNQVEAEMTRYGAYGGHHLGTARMGADPADSVVDAQCRLHE
                     TDNVYIASAAVFPTSSQANPTLTVVALALRLADHLRKTYRRAGSAAHP"
     gene            4286..>4723
                     /locus_tag="VGC69_03465"
     CDS             4286..>4723
                     /locus_tag="VGC69_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY0294376.1"
                     /translation="MNPKVELFGLDFSALTSAQAMDALAAAAVRRDGRAQVIVPTNVD
                     CLVRLEANLDFKFHYYAKADFILADGMPVVWASRLLGRPVPERVTGADLFVGLCQRAQ
                     AEGWQVVVLGGDPQIEAEIRQGFARTFPGLRVEIVSPSMRYDPE"
BASE COUNT          726 a         1562 c         1671 g          764 t
ORIGIN      
        1 cggcaccgac ctcagcatga gcgacaccat ctacggcggc aatctcgagc ccctgctcaa
       61 gcgcggcaag atccgcatgg ccgacatcga ccgcgcctgc cgcgacgtgc tgcgcgtcaa
      121 atatgacctg ggcctgttca aggacgccta ccgcgacatc ggcgtggcgg ccgacgaccc
      181 gccggacacc tatgccgaga gccgcctgca ccgccccgag gcccgcgacg tggcgcgcga
      241 gagcctggtg ctgctcaaga accagaacca ggcgctgccg ctgaaaaagc agggcaccat
      301 tgccctcatc ggtccgctgg ccgatgacca ggtcgacgtc atgggcaact gggccggcgc
      361 gggccgcatc ccccagacca tcgccctgct gcaaggcgtg aagaacgcgg tgggcgacaa
      421 ggcccgggtg ctctacgcca agggctccaa catgaccgac gaccccgatc tcgtgcagta
      481 cctcaaccag tacaaccacg acgtgcaggt cgatccgcgc agcgaggacg acatggtcaa
      541 ggaggccgtc gaggccgcac ggcaggccga tgtggtcgtg gccgcggtgg gcgagtccca
      601 gggcatggcc cacgaggcct ccagccgcag cgacatcacc ctgccgcccg tgcaggtgcg
      661 tctgctcgag gcgctcaagg ccaccggcaa gcccctggtc atcgtgctga tgaatggccg
      721 cccgctggcc ctggtgcgcg aggccggcat ggccgacgcc ctgctcgaga cctggttcgc
      781 cggcaccgag ggcggcaatg ccatcgccga cgtcctgttc ggcgacgcca acccgtcggg
      841 caagctgccc atgacctttc cgcgcgcggt ggggcaggtg ccgctgtact acaaccacct
      901 gcccaccggc cggccgttcg acccagtgca tcccgacaag tacacgtcgc gctatttcga
      961 catcaccaac gggccgctgt ttccctttgg ctacggcctg agctacacca ccttcgatgt
     1021 ctcggacgtc agcctgtcgg cgcccgcgat gaaggctggc ggcaccatgc aggcccgcgt
     1081 cacggtgacg aacacgggcg atcgcgaagg cgcgacggtg gtgcagctct acctgcagca
     1141 gcccgtggcc tcggtcagcc gcccggtgag ggagctcaag ggttttcgcc ggatcagcct
     1201 caagcccggc gaatcgcgcg tggtgagctt cgacatcacc gacgacgacc tggctttcta
     1261 caacgccggg atgaagcgcg tggtcgagcc cggcaagttc aacgtctatg tcgggctgga
     1321 ctccgatgcg gacaagctcg ccagcttcaa tcgtcaataa accgcacatt tcttgcgggg
     1381 tgttgcctgt ttgacgtttt ggttacgcgt cgtcatccct gattgcgcgc cgcggcccta
     1441 gactggcctt ttcgaatctt tttataggcg tcaggacatg aaagctttat tggcgtgcgc
     1501 agcggcagcg gtgatgctgt ccggctgtct ggtgtatgac ggcccggatc attggcacca
     1561 tcggtactcc gccggccatt acggctatga gcagcgcggg cattaatcgt cctggcgcaa
     1621 tgcctcgagc cagggcgtcc attgcaggcc caggtcgcgc tgcgccttct ccgatgccag
     1681 gcggatgtcc tgctgccaga gccgccgcag cgtgggtgtg atgggcggcg gcagaggcgg
     1741 caggccgatc ttgcggccgg cgatttcggc gatcttgagg gctggcgcga gcaccttggc
     1801 ttccagggcc agccgccgcg cgccgatgcg ggacacgggc gtggcgccca ggatgcgcgc
     1861 gtaggcgatg aagtattcat tccaggtcgg cgcctgggcc atggacaggt tgtaggcctg
     1921 gccggcggcc tcgggccggc gcagggcggc cagcacggcc tgcgcgacgt cgcgcacatg
     1981 caccagattg ctgtagccgt cgccggctgc gccgagatcg ccgatgcggt gcgcgcgcaa
     2041 tagccgggcg atgcgcgccg accattgcgg gctgccagcg ccgtagacgc agccgggccg
     2101 cagcaccacg gtttgcgcgt attccttcaa tatgcgttcg gtctcggcct tggcggcgga
     2161 gtaggggccc aggtcgccca gcagggtctg gtcttcgtcg atcaggcctt ctgctgaacc
     2221 gtaggcggcc atcgaactga agtgcaccag gcgcatgggc cggccctaca gcgccgtgcg
     2281 cagcgcctgg gcgttggcga ccatgtccgg cgccgccgcc gccatggcgt tgacgacggc
     2341 gtcggcctgc cgcatggccg ctgccagggc cgccgcatcg gtcgcgtcca gggccacggc
     2401 gccgcgccgc gccgcggcga tgggttcggc ccagtccgtc gcggccaggg cctggacgat
     2461 gtgctggccg acatagccgg tggcgcccag caccaatacg cgtagcttgt tcatgggtga
     2521 gcggcgctcc ctgcccgcct ataggtttta cgcaagtgat cggccagccg cagggccagg
     2581 gccaccacag tcagggtggg attggcctgg ctcgacgtgg ggaagacggc agcgctggcg
     2641 atatagacgt tgtcggtttc gtgcagccgg cattgcgcat cgaccacgct gtcggccggg
     2701 tcggcgccca tgcgggccgt gcccagatgg tggccgccgt aggcgccata gcgggtcatc
     2761 tcggcttcga cctggttcgg ttcgtagtcg aagctgccga cgccggtttg ccggaaggcc
     2821 tgagccagcg cggccagggc cgcccgcacc gtctgcacgt cgcgttcggt atagcgccag
     2881 tccacgtcga tgcgcggcat gcccagggcg tccacgccct ggcccagttt tacgcggctg
     2941 gacgggttgg gttcctgctc ggcgtgaaaa tccaggctga aacgcaggtt tttgggccgc
     3001 atgacgatgg acggaaactt gcgctcggcc agggtgcggc ggcgcagcca gttccacaga
     3061 aaggccgccg tgcccggcgc gtccagcacc acgttgcgca ggtgcgccag cgtggcgccg
     3121 cggcccggct cgtcgccgta gagccgcttg gcgtattcgt agggcacgat ggggcgcgcc
     3181 aggtacagcg ccgacagaat gccgctgccg tggcccgggt cggggatgcg gggatgatgc
     3241 aggcgggcga tgaagttgcc ggcctgcaag gcgcgctggg cctgcgggcg caaggccagc
     3301 cggcggcggc aatagatgcc ttcgtcggaa atctcgtagc catgccatac cgagctggcg
     3361 cgggacaggt cggccgtgcc catggtgccg gcgatgtggc tcatgtaata gcggcccacc
     3421 acgtcgcgcg cgttgcccag gccgcgtccg ctcgcgccgg ggctggccag cagcagcctg
     3481 gcgacttcca gcccgccggc ggcgagcaca taggcccgcg cctgcacggt gaaatggttg
     3541 ccggccaggg tgcgcaccgt cgccgggcca gccagccgtc cgtcggccgc ggtggacagg
     3601 ccgaccaggt tggcgttgaa cagcacgcgc gccgggccgc gttccaggcg ccgccggtag
     3661 cgactgccga aatccgtcgg gcaactgaag cgttccagcg tattggtgga gaaggcgtcg
     3721 ctgtcgaagc cgcctatcat ggggcggacg ggatgcgtga aagccgtctc gacggtgtag
     3781 gcgaagtcgc cggcttcgca cagcaggttg gcgcgcgggt aatagggcag caagtcgtcc
     3841 gggcctatgg gccagccgct gtgggcgatg tagctgcgcg gttcgaagtc gatggggtcc
     3901 agcgggatgc agcgtccgcc ccacagggtg gtcgagccgc cgaagcggcg cacgcggtaa
     3961 tgatcgggcg gcgagtgcag gcgggcgtcg gcgaccgtgc cttcgtagag cgcctgggtc
     4021 gcgtcgtcgg gctggtcggc gccgctttcg agcaggatca cgtccaggcc gctgtcctgc
     4081 agggccagcg ccatggcgat gccggccgcg ccggctccga cgatgcacag atcggtgtgg
     4141 agcgtagcgc cggcgggggt ccggttggcg tcctcgatca tgtggtgcgc acagtcgtgg
     4201 gttgtggcga tgccgcgatc ataactgatg tcccacgccc cgggtggtgt ataaaacgcc
     4261 accgggactc aagcggacaa acaccatgaa ccccaaggtt gagctgttcg gactggattt
     4321 ctccgcgctg acatccgcgc aagcgatgga tgccttggcc gcggccgcgg ttcggcgcga
     4381 cggccgcgcg caagtgatcg tgccgaccaa tgtcgattgc ctggtccggc tcgaggccaa
     4441 cctcgatttc aagttccact attacgcgaa ggcggatttc atcctggccg acggcatgcc
     4501 cgtggtctgg gccagccgct tgctgggccg gcccgtgccc gagcgggtga cgggcgcgga
     4561 cctcttcgtc ggcctgtgcc agcgggcgca ggccgagggc tggcaggtcg tggtgctggg
     4621 tggcgacccg caaatcgagg cggaaatccg ccagggtttc gcccgcacct ttccgggcct
     4681 gcgcgtcgag atcgtcagcc cctccatgcg atacgatccc gag
//
LOCUS       DASWPO010000057         3910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2333369, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000057 DASWPO010000000
VERSION     DASWPO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333369"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="fdhE"
                     /locus_tag="VGC69_03470"
     CDS             <1..942
                     /gene="fdhE"
                     /locus_tag="VGC69_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEY0294377.1"
                     /translation="EDLDHNAIVRVRLPARATVFAARASRLRQLAESSPVADYLGLMA
                     HLADAQQAVLAGCEAPGASPERINLAQAHGMPPLQAVGWRRDPLWTGLLRQILMRLEM
                     TAGVPDEALEVCRDLRRALQEQPGVIEAMADAILAERDSEVDSAAAPFVMAALQVYWT
                     DLASRFDAKQLPVASPFGVCAVCGSLPVASVVRVGGQFDGYRYLCCSLCASEWHMVRV
                     KCSHCEDTETVAYHAIEGGAEAIRAESCDNCHTYRKIFYQEKDFDVEPVADDLASLLL
                     DVLMGEAGYVRASGNPLLWQGETDADEDGPGREDGAA"
     gene            939..2378
                     /gene="selA"
                     /locus_tag="VGC69_03475"
     CDS             939..2378
                     /gene="selA"
                     /locus_tag="VGC69_03475"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY0294378.1"
                     /translation="MTAQAATPSAAVLAAPADIPALDRVLAAAPAQALLDTYGRTRVV
                     AALREHMAALRAEALAGGLPAARVQPDAIAGEAGEALRRAAQSRLRAVYNLTGTVLHT
                     NLGRALLPDEAVQAVMHAMTAPANLEFDLESGGRGDRDDLIESLLRELTGAEAATVVN
                     NNAAAVLLMLNALADRREVVVSRGELVEIGGAFRIPDIMKRAGAKLVEVGTTNRTHAA
                     DYDTAIGPRTAMLMKVHCSNYAVSGFTKSVDVAEVAKIAHARGLPMTVDLGSGTLIDL
                     AQWGLPREDTVRDTVQAGADLVTFSGDKLLGGPQAGLLVGNADLIRKIKKNPLKRALR
                     VGKLTLAALEPVLALYRSPEFLAERLTTLRLLTRAQRLMQAAAERLRDTVQHAVGADY
                     QVEVAAMFSQIGSGALPVDQLPSCGLCIRHAGPGRAGRHLARLEAGLRGLPRPVIGRL
                     GDDALWLDLRCLEERDEAGFVAQLDRYKK"
     gene            2433..>3910
                     /gene="selB"
                     /locus_tag="VGC69_03480"
     CDS             2433..>3910
                     /gene="selB"
                     /locus_tag="VGC69_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEY0294379.1"
                     /translation="MIIGTAGHIDHGKTTLVRALTGVDTDRLKEEKARGISIELGYAY
                     APLPNGDVLGFIDVPGHEKLVHTMAAGASGIDFGLLVVAADDGIMPQTHEHLAILGLL
                     GVKRGAIVLTKADRCDDEKRQYVEHDLAQLAAGTFLQGAPVFTTLAHRPGDPGVARLR
                     TYLDAQAQAMPLRAADGLFRLAVDRVFTLAGHGTVVTGTVHAGRTRAGDDAADLRLMP
                     AGVPVRVRSIHAQNRSSPEGSAGQRCALNLAGIDKDAIARGDWIADARGFLPSRHVDV
                     ALQWLVSADGPLQAWAPLHVHIGAAHHVAHAVPLVPEILAPGRRGRVQLVFDAPVCAM
                     PGDRYIVRNAQASRTVGGGVVLDPNAPDRKRRAPQRLAWLDAVSAYLSGQGLDAVLQQ
                     APLGLDEAALQRLSGQSAASIAVPAGAQWVAARGSRTLILDGHWDALCDRAAGALATF
                     HQQSPDEPGLDAARLRRIAAPTAAEALWQAVVERLLREGRLVR"
BASE COUNT          555 a         1387 c         1423 g          545 t
ORIGIN      
        1 gaagacctgg atcacaacgc cattgtgcgg gtgcgcctgc ccgcgcgcgc cacggtgttc
       61 gccgcgcgcg caagccggct gcgccagttg gccgaaagca gcccggtggc cgactacctg
      121 gggctgatgg cccacctggc cgacgcccag caggcggtgc tggccggctg cgaggcgccc
      181 ggcgcgtcgc ccgagcgcat caacctggcc caggcgcacg gcatgccgcc gctgcaggcc
      241 gtgggctggc ggcgcgaccc gctctggacg ggcttgctgc gccagattct catgcgcctg
      301 gagatgacgg ccggcgtgcc ggacgaggcc ctcgaggtct gccgggacct gcggcgcgcc
      361 ctgcaggagc aacccggcgt catcgaggcc atggccgatg ccatcctggc cgagcgcgac
      421 agcgaagtcg acagcgcggc cgcgcctttc gtcatggcgg ccctgcaggt ctattggacg
      481 gacctggcca gccgcttcga tgccaagcag ctgcccgtgg ccagcccttt cggcgtgtgc
      541 gccgtatgcg gcagcctgcc cgtggccagc gtggtgcggg tgggcgggca gttcgacggc
      601 taccgctacc tgtgctgctc cctgtgcgcc agcgaatggc atatggtgcg ggtcaagtgc
      661 tcgcattgcg aagacaccga gacggtggcc taccatgcga tcgagggcgg cgcggaggcg
      721 atccgggccg agtcctgcga caactgccat acctaccgca agatcttcta ccaggagaag
      781 gacttcgacg tcgagccggt ggccgacgac ctggccagcc tgttgctcga cgtgctgatg
      841 ggcgaggccg gctatgtgcg cgccagcggc aatccgcttc tgtggcaggg cgaaaccgat
      901 gccgacgaag acggtcccgg ccgagaggac ggcgcggcat gacggcgcag gccgccacgc
      961 cgtccgcggc cgtgctggcc gcgcccgcgg acattcccgc gctggaccgc gtgctggccg
     1021 ccgcgccggc gcaggccttg ctcgacacct acgggcgcac ccgggtcgtg gccgcgctgc
     1081 gcgaacacat ggcggccctg cgcgccgagg cgctcgccgg cggcctgccg gccgcgcgcg
     1141 tgcagcccga cgcgattgcc ggggaggcgg gcgaggcgct gcggcgcgcc gcgcagtcca
     1201 ggctgcgcgc ggtctacaac ctcaccggca ccgtgctgca taccaacctg ggccgcgcct
     1261 tgctgcccga cgaggccgtg caggcggtga tgcatgccat gaccgcgccg gccaacctgg
     1321 aattcgacct ggagagcggc ggccgcggcg atcgcgacga cctgatcgag agcttgctgc
     1381 gcgagctcac cggcgccgag gccgccaccg tcgtcaacaa caatgcggcg gccgtgctgc
     1441 tcatgctcaa tgccttggcc gacaggcgcg aagtcgtcgt ctcgcgcggc gaactggtcg
     1501 agatcggcgg cgccttccgc atccccgaca tcatgaagcg ggccggcgcg aagctggtcg
     1561 aggtgggcac caccaatcgc acgcacgcgg ccgactacga caccgccatc ggcccgcgca
     1621 cggccatgtt gatgaaggtc cattgcagca actatgccgt ctccggattc accaagagcg
     1681 tggacgtggc cgaggtggcg aagatcgcgc atgcgcgcgg cctgcccatg acggtggacc
     1741 tgggcagcgg cacgctgatc gacctggccc aatggggcct gccgcgcgag gacacggtgc
     1801 gcgacaccgt gcaggccggg gcggacctcg tcaccttcag cggcgacaag ctgctgggcg
     1861 gcccgcaggc gggcctgctg gtgggcaatg ccgacctgat ccgcaagatc aagaagaacc
     1921 cgctcaagcg cgccctgcgc gtgggcaagc tcacgctggc cgcgctcgaa ccggtgctgg
     1981 ccctgtaccg ttcgccggag ttcctggccg agaggctcac cacgctgcgc ctgctcactc
     2041 gtgcgcagcg gctgatgcag gcggccgccg agcgcctgcg cgataccgtg cagcatgccg
     2101 tgggggcgga ctaccaggtc gaggtcgccg ccatgttcag ccagatcggc agcggcgcct
     2161 tgccggtgga ccagttgccc agctgcggcc tgtgcatccg ccatgccggg ccggggcgcg
     2221 cgggccggca cctggcgcgg ctcgaggccg gattgcgcgg cctgccgcgc ccggtcatcg
     2281 gccggctggg cgacgatgcc ctgtggctgg acctgcgctg cctcgaggaa cgcgacgagg
     2341 ccgggttcgt ggcgcagctc gataggtata agaaataggg atttctatac ctatccaggc
     2401 caacatgcac agaatttgct gaacatcgaa tcatgataat aggcactgcc ggccacatcg
     2461 atcacggcaa gaccacgctg gtgcgggcgc tcaccggcgt cgacaccgat cgcctcaagg
     2521 aagaaaaggc gcgcggcatt tccatcgagc tgggctacgc ctatgcgccg ctgcccaatg
     2581 gcgacgtgct tggcttcatc gacgtgccgg gccacgagaa gctggtccac accatggcgg
     2641 ccggcgccag cggcatcgat ttcggcctgc tggtggtggc cgccgacgac ggcatcatgc
     2701 cgcagacgca tgagcacctg gccatcctgg gcctgctggg cgtgaagcgg ggcgccatcg
     2761 tcctgaccaa ggccgaccgc tgcgacgacg agaagcggca gtacgtcgaa cacgacctgg
     2821 cgcagctggc ggccggcact ttcctgcagg gcgcgcccgt cttcacgacc ctggcccacc
     2881 ggcccggcga tccgggcgtg gcaaggctca ggacttatct cgacgcgcag gcccaggcca
     2941 tgcccctgcg ggcggccgac ggactgttcc ggctggcggt cgaccgcgtg ttcaccctgg
     3001 cgggccacgg cacggtcgtg acgggtaccg tgcatgcggg ccggacccgg gccggcgacg
     3061 acgcggccga tctgcgcctg atgccggcgg gcgtgccggt gcgggtccgc agcatccacg
     3121 cccagaaccg gtccagcccc gaggggtcgg ccgggcagcg ttgcgccttg aacctggccg
     3181 gcatcgacaa ggatgccatc gcgcgcggcg actggatcgc cgacgcgcgc ggcttcctgc
     3241 cttcgcgcca tgtggacgtg gcgctgcaat ggctggtgtc ggccgacggg ccgctgcagg
     3301 cctgggcgcc gctgcatgtg cacatcggcg cggcgcacca tgtggcccac gccgtgccgc
     3361 tggtgcccga gatcctcgcg ccgggccggc gcggccgggt gcagctggtg ttcgacgcgc
     3421 cggtctgcgc catgccgggc gaccgctata tcgtgcgcaa cgcgcaggcc agccgtacgg
     3481 tgggcggcgg cgtggtgctc gaccccaatg cgccggaccg caagcgccgc gcgccgcagc
     3541 gcctggcctg gctggacgcg gtgtcggcct acctgtccgg gcagggcctg gatgccgtgc
     3601 tgcagcaggc gcctctgggc ctggacgaag ccgccttgca gcgcctgtcc ggccagtccg
     3661 ccgcaagcat cgccgtgccg gccggcgcgc aatgggtggc ggcgcgcggg tcgcgcaccc
     3721 tgatcctgga cggccattgg gatgccctgt gcgaccgcgc cgccggcgcg ctggcgacgt
     3781 ttcaccagca gtcgcccgac gagcccggcc tggatgcggc gcgcctgcgc cgcatcgccg
     3841 cgcccacggc cgccgaagcc ctgtggcagg ccgtggtcga gcggcttttg cgggagggcc
     3901 gcctggtgcg
//
LOCUS       DASWPO010000058         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1441387, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000058 DASWPO010000000
VERSION     DASWPO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441387"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1935
                     /gene="htpG"
                     /locus_tag="VGC69_03485"
     CDS             37..1935
                     /gene="htpG"
                     /locus_tag="VGC69_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY0294380.1"
                     /translation="MSQTASSPSETLGFQAEVKQLLHLMIHSLYSNKEIFLRELVSNA
                     SDACDKLRFEAIDHPELLAGDSELAIRVAYDKAARTITISDNGIGLSRDEAIANLGTI
                     ARSGTREFFSQLTGDKQKDAQLIGQFGVGFYSSFIVADKVTVVSRRAGSEAAIRWESD
                     GEGEFTIADAERATRGTDVTLHLRADEDELLNGWKLREILRRYSDHISLPIRMAKEDW
                     DAEKSEQVQKDELEAVNQANALWTRGKADITDEQYREFYKAISHDFDDPLAWTHNRVE
                     GRSEYTQLLYVPKHAPFDMWDRDARRGVKLYVKRVFIMDDAEQLLPSYLRFVRGVIDS
                     ADLPLNVSREILQESRDVRAIREGSARRILSLLEDLAENKQDEYAAFWTEFGQVLKEG
                     TGEDPSNLERIAKLLRFASTQSGDAAQTVALADYVSRLKEGQDKIYYVTADSHSAASS
                     SPHLEIFRKKGIEVLLMSDRVDEWMLSHLREFEGKSLVSVAKGGLDLADLADDEEKKR
                     QTEVAEEFKPLIERLQEALKDQVKEVRVTLRLVDSPACVVVGQNELSPHLLRMLKAAG
                     QEAPDVKPVLEINPEHALIARIGKATDEEFSDWAKLLLDEAMLAEGAQITDPAAFVKR
                     MNALLLKA"
BASE COUNT          403 a          690 c          634 g          315 t
ORIGIN      
        1 ccctcctttg tttatctccg tattgttttg accatcatga gccagaccgc atcctccccc
       61 tccgaaaccc tgggttttca ggccgaagtg aagcaattgc tgcacttgat gatccactcg
      121 ctctacagca acaaagagat cttcctgcgc gagctggtgt ccaatgcctc ggacgcctgc
      181 gacaagctgc gcttcgaggc catcgaccat cccgagctgc tggccggcga tagcgagctg
      241 gccatccgcg tggcctacga caaggcggcg cgcaccatca ccatctccga caatggcatc
      301 ggcctgtcgc gcgacgaggc catcgccaac ctgggcacta tcgcccgctc gggcacgcgc
      361 gagttcttct cgcagctcac gggcgacaag cagaaggacg cccagctcat cggccagttc
      421 ggcgtgggct tctattcctc cttcatcgtg gccgacaagg tcacggtggt gagccgccgc
      481 gccggttccg aggccgccat ccgctgggag tccgacggcg agggcgagtt caccatcgcg
      541 gacgccgagc gcgccacgcg cggcaccgac gtcaccctgc acctgcgcgc cgacgaagac
      601 gaactgctca acggctggaa gctgcgcgaa atcctgcgcc gctactccga ccacatctcc
      661 ctgcccatcc gtatggccaa ggaagattgg gatgccgaga aaagcgagca agtgcagaaa
      721 gacgagctcg aggcggtgaa ccaggccaat gcgctgtgga cgcgcggcaa ggccgacatc
      781 acggacgagc aataccgcga gttctacaag gccatctcgc acgacttcga cgacccgctg
      841 gcctggacgc acaaccgcgt ggaaggccgc agcgaataca cccagctcct gtacgtgccc
      901 aagcacgcgc ctttcgacat gtgggaccgc gatgcgcgcc gcggcgtcaa gctgtacgtc
      961 aagcgcgtgt tcatcatgga cgacgccgag cagctgctgc cctcgtacct gcgcttcgtg
     1021 cgcggggtga tcgactcggc cgacctgccg ctgaacgtct cgcgcgagat cctgcaggaa
     1081 agccgcgacg tgcgcgccat ccgcgaaggt tcggcccggc gcatcctgtc gctgctggaa
     1141 gacctggccg agaacaagca ggacgaatac gccgctttct ggacggaatt cggccaggtg
     1201 ctcaaggagg gcacgggcga agacccgtcc aacctcgagc gcatcgccaa gctgctgcgc
     1261 ttcgcctcga cgcaatcggg cgacgccgcg caaaccgtgg cgctggccga ctatgtgtcg
     1321 cgcctgaagg aaggccagga caagatctac tacgtgacgg ccgattccca ttcggccgcc
     1381 agcagcagcc cgcacctgga aatcttccgc aagaaaggca tcgaagtgct gctcatgtcc
     1441 gaccgggtgg acgagtggat gctttcccac ctgcgcgaat tcgaaggcaa gtcgctggtg
     1501 tccgtggcca agggcggcct ggacctggcg gacctggccg acgacgagga aaaaaagcgc
     1561 cagacggaag tggccgagga attcaagccg ctgatcgaac gcctgcagga agccctcaag
     1621 gaccaggtca aggaagtgcg cgtcaccctg cgcctggtcg attcgcccgc ctgcgtggtg
     1681 gtcggccaga acgaactcag cccgcacctg ctgcgcatgc tcaaggccgc cggccaggaa
     1741 gcccccgacg tcaagcccgt gctggagatc aaccccgagc acgccctgat cgcgcgcatc
     1801 ggcaaggcga cggacgaaga gttcagcgat tgggccaagt tgctgctcga cgaggccatg
     1861 ctggccgaag gcgcccagat caccgacccc gccgccttcg tcaagcgcat gaacgcactg
     1921 ctgttgaagg cctgagatcc agggcaactt gtaagtcagc cggtccgggc ttctcctcgc
     1981 agccttttga ggcgggggca gccccgaagg ggccgagggc gcctgatggc gcgcccgagc
     2041 cc
//
LOCUS       DASWPO010000059        30755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2869604, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000059 DASWPO010000000
VERSION     DASWPO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 30755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30755
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2869604"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1712)
                     /locus_tag="VGC69_03490"
     CDS             complement(183..1712)
                     /locus_tag="VGC69_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY0294381.1"
                     /translation="MPNPIVVAKNAHAELALLPALANRHGCITGATGTGKTVTLQVLA
                     EAFSRMGTPVFMADVKGDLTGISQAGTGSPKLQERLARLGLPEPAWGACPVALWDVFG
                     EQGLPVRATVSDMGPLLLARMLELNDVQEGVLALVFRVADDEGQLLLDLKDLRAMLQN
                     VADRAAELKTRYGNVSDASVGAIQRSLLQLESQGADKFFGEPMLDVADLLGTDAQGRG
                     IVNVLAADKLMQAPRLYGVFLLWLLADLYEKLPEVGDLPQPKLVFFFDEAHLLFDEAP
                     SALMQKIEQVVRLVRSKGVGVYFATQNPLDIPDRVLGQLGNRVQHALRAFTPRDQQAV
                     KTAADTMRPNPGLDIQAAIGELGVGEALVSFLDDKGIPTPTERAWIMPPGGRIGPITP
                     AERQALRQGSPLAGKYEQTVDRESAYERLTGRATASVPDSGTGAAPVPPAAGQPAAPA
                     QQGGGGGLMGGVSNVLFGHVGPRGGQHDGLVQVAAKSLARQVTTQLVRGVLGSLLGGR
                     R"
     gene            1861..2592
                     /locus_tag="VGC69_03495"
     CDS             1861..2592
                     /locus_tag="VGC69_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0294382.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKLWTKIIREITVAARAGGADPD
                     ANPRLRMAWDKATDANMPKDNVQRAIARGAGGADGANYEEARYEGYGVGGAAVIVDCT
                     TDNRTRTAAEVRHAFTKNGGNLGTPGSVEFMFKHCGQFLFAPGNSEDKVMEAALEAGA
                     EDVITDDEGGIEVICAPGDYPAVKQALGAAGLKAEVDGVIMKALNETELTGDDAIKMQ
                     KLLDALEDLDDVQEVYTNVVFDEAQ"
     gene            2589..3893
                     /gene="purD"
                     /locus_tag="VGC69_03500"
     CDS             2589..3893
                     /gene="purD"
                     /locus_tag="VGC69_03500"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0294383.1"
                     /translation="MKLLVIGSGGREHALAWRLAQSPRVHRVYVAPGNGGTGLDSLLD
                     NAPYTTAEELADFVEREDIGITVVGPEAPLAAGVVDVFRARGLKIFGPTKAAAQLESS
                     KDYAKAFMMRHKIPTARYQTFTDPAQAHAYIDQEGAPIVIKADGLAAGKGVVVAMTLE
                     EAHAAVDGMLSGGTMGIAGARVVIEEYLAGEEASFIVMSDGRNVLALATSQDHKRLLD
                     GDAGPNTGGMGAYSPAPIVTPELHHRILKEIILPTVHGMALDGIPYTGFLYAGLMIGP
                     GSDATRLVKTIEFNCRLGDPETQPIMMRVKSDLLDAIEHAIDGTLDQADLAWDRRTAL
                     GVVMAAHNYPGTPRSGDAITGLPAPAEDCMVFHAGTTLDGGTLKTSGGRVLCVTALGD
                     SVRMAQDRAYKAASAINFDGAQYRRDIGWRALKPSQQRKPQP"
     gene            3918..4820
                     /gene="hemF"
                     /locus_tag="VGC69_03505"
     CDS             3918..4820
                     /gene="hemF"
                     /locus_tag="VGC69_03505"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY0294384.1"
                     /translation="MDAAPVRDYLTNLQARIVAALEKAGGDTFRTDAWQRPEGGGGLS
                     RLIEGGQLLERGAVLFSHVTGTLLPPSASAHRPDLAGRAWEAMGVSMVLHPRNPYVPT
                     THMNVRMFRAVARPGYDEREVFWFGGGLDLTPYYPFEADACHFHGVCRDALSPFGPDY
                     YPHYKRWCDEYFFLKHRNEARGIGGIFYDDLNEPDFDTAFALTRSVGDAFLPAYLPIV
                     ERRCKTPYGQRERDFQSYRRGRYVEFNLVHDRGTLFGLQSGGRTESILLSMPPIANWR
                     YDWHPEPGSPEAALQDFLVPRDWV"
     gene            4823..5413
                     /gene="nadD"
                     /locus_tag="VGC69_03510"
     CDS             4823..5413
                     /gene="nadD"
                     /locus_tag="VGC69_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEY0294385.1"
                     /translation="MAKRIGLLGGSFDPVHEAHIALALAARQALELDEVQLIPAAMPW
                     QRGDLKAAAKHRLRMLDIVAAKHPGLTVNPIELERGGTTYTVDTLRALPAGPDYIWLL
                     GADQLANFCTWRNWREITERVDLAVAVRPGTPLHAPDELADWLATRQSPLQQLPFQPM
                     DVSASDIRTRLTSGASTAGLLDPDVATYIATHKLYV"
     gene            5427..5813
                     /gene="rsfS"
                     /locus_tag="VGC69_03515"
     CDS             5427..5813
                     /gene="rsfS"
                     /locus_tag="VGC69_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY0294386.1"
                     /translation="MDIQKLQRAVIDALEDVKAQDIKVFNTMHLTSLFDRVVLASATS
                     NRQTRALAASVANRGRELKLTGITIEGEDTGEWVVVDLGDIVIHIMQPAIRQYYNLEE
                     IWGDKPVRVKLLPESTRPAMGSEASF"
     gene            5940..6410
                     /gene="rlmH"
                     /locus_tag="VGC69_03520"
     CDS             5940..6410
                     /gene="rlmH"
                     /locus_tag="VGC69_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY0294387.1"
                     /translation="MKLIILAVGNRMPDWVQTAWDDYAKRLPADCALELREIKPEPRT
                     SGKTPAQMMAAEAKRIEAALPAGALRLALDERGRDLTTMALSKQLEQWRADGRDVALL
                     VGGPDGLDAALKASCDGQLRLSSLTLPHPMVRVLLAEQLYRAWAIMTNHPYHRA"
     gene            6478..7080
                     /locus_tag="VGC69_03525"
     CDS             6478..7080
                     /locus_tag="VGC69_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY0294388.1"
                     /translation="MSMPRLYLASASPRRRELLLQIGLAHEVLRVPAPPGDDEPQLPG
                     ESPAGYVLRTAREKAQRGEQWMRSRALPALPVLAADTTVVLDGAVLGKPADRDDALRI
                     LQALSGTTHEVRTAIVLAVQGRLLEAVSVTRVAMRATSAAERERYCDSGEPYGKAGAY
                     GIQGLASLFIEHIAGSYSGVMGLPLFETARLLAQAGIELP"
     gene            complement(7056..7970)
                     /locus_tag="VGC69_03530"
     CDS             complement(7056..7970)
                     /locus_tag="VGC69_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0294389.1"
                     /translation="MKNQAGWLRLSDALLLAVAAVWGSTYAVVKSTLVFYSVLGVLAL
                     RFGLAFVLLLPALRGLRALPRSSVWGALGCGAVLFGIFLAETFGVMLTEASTAAFLIS
                     LCVVLTPWAEWALLHRRPARKEWVAAALSLVGAWLLSGAGARLQAGDALILLAALLRA
                     LHGCVMKRVMRDARLTPTMLTAVQSGVVAGGGAIAALAAGQWGPLPAWPSQDGFWVGM
                     AYLALVCTVFAFFVQSFAMQRSHPTRVSLLMGSEPAFGALVACLWLGERLTPLAWLGG
                     ALMVGVSLWAVLPGGARPGPAQGSSMPA"
     gene            8090..9013
                     /locus_tag="VGC69_03535"
     CDS             8090..9013
                     /locus_tag="VGC69_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294390.1"
                     /translation="MHPDTLSALLPDLATFARVVDAGNFSEAARQLGSTPSTISRQIK
                     RLETALGTRLLERSTRSVRVTESGEQVYRRCRDMLEAATSAIDAASRLAAQTRGLVRF
                     SAPISYAHYVIHPLMPAFLRAHEAVDVQLIFTDRELDPLQDDVDLVIRLTRHPPPGLA
                     ARPLGEVRWILCASPGYLQARGAPRQPADLAAHDCIYLGETPQDRHWTLHRGNTVQTV
                     DVRGRYQANHADARLDAALRHLGIASLPEFLAAEALRSGALARVLPDWDFEPAAYTGP
                     VWLLYPPNRFLPPKVRALIDHLAAHLPGNQA"
     gene            9277..10665
                     /locus_tag="VGC69_03540"
     CDS             9277..10665
                     /locus_tag="VGC69_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY0294391.1"
                     /translation="MGKTILFVLGAVLLAYGIGAGVGFMMVERISREQWWSQAATYTQ
                     ISATALRSIYTAISVETNADGQITRIVSESPIGDADSILQTGFSPVDVLALAAAQTRH
                     NVWLFRCQEGHASNMSMVSALDGTQGAPAAGDGQTSLTPDEIKNPYVGFARINGAEYF
                     VSAIPIATHAGKQTGVLISSIGLTQSLYQTRNALIRNSLLVLLGVLLAATVAIIWLMH
                     RLFRPVPALIQSLMRITHGDIQSVTPYQGRPDEIGRLATAIETLRNAVAEREQLLSIQ
                     EAAMRLEHMAHHDALTGLPNRAFLDRTLSHAVATLPSGARFNVMLFDLDRFKEVNDSY
                     GHAIGDALLVAISDRLNLLLGPEDVAARLGGDEFVVVQKVARDALLEASKLASRIIET
                     LKRPFLVDGRELHIGASVGITCAPSDGDNAHDLLKNADTALYAAKNGGRGRFTFYESR
                     MSMDARTHTPLS"
     gene            10689..11633
                     /locus_tag="VGC69_03545"
     CDS             10689..11633
                     /locus_tag="VGC69_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0294392.1"
                     /translation="MHLTRIMAAWPRKRLCSWQRRLQSQAAFAALAILPVAQQAQATD
                     TLAKIASTHRIIIGHRQSELPFSYVANGKVIGYAIDICQKVVEGLQKQLNLDHIEIDY
                     QLVATATRFIQLNSGNIDLECATTTNNAERRKIAEFSYPYFFTATRYVAKKQAGLHTI
                     ADLAGHSVVSTTGTIDVDQLNAVNLAQHLNISVLLRRTHSDSFQLVENGQASAFVMDD
                     ILLAGLVASSGHPGDYTISQDAFNRPEPYGILIPAGDIAFKSAVDRVLRTLYASHEID
                     QLYAKWFMQPIAPDGRNLKLPMSPELRDWLAAPREYLD"
     gene            11648..12550
                     /locus_tag="VGC69_03550"
     CDS             11648..12550
                     /locus_tag="VGC69_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0294393.1"
                     /translation="MRLPSPSRRFCLGLAAALLTAGLSHAQTPPASTLERIRQTGVLR
                     IGFGYAQPFAYIEANGTVTGYSIELCGRLAETLRVQLALPRLDIQYSRLAPNNRISQL
                     NSGAIDIECGASTNSPERRASVTFGPSHFITDSRYVSLAANALRTIDDLRGKSVAVVL
                     GTTNVGDIARLNRERRLNLSVVTTHTNQDAFDLITQGKVSAFPMDDVLLHMLVGASGN
                     PDRYAVSDETLAPPQAYGFMMRRNDPDFVQAVDAALRNIYASPDMPALYDRWFNEPLP
                     GSGVNLHLPMSPTLRRALFGADAG"
     gene            complement(12576..13883)
                     /locus_tag="VGC69_03555"
     CDS             complement(12576..13883)
                     /locus_tag="VGC69_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY0294394.1"
                     /translation="MIDLDQANTQQPSAPARKPKFYQILYVQVIAAILIGIALGHYVP
                     DTAVAMQPLGDGFIKLVKMIIAPVIFLTVTTGIAAMSDLKKVGRVVGKAMIYFIVFST
                     IALVIGMIVSHLIQPGGGMHINPASLDQNQVKGYVSKAHDSTVSGFILNIIPTTLLSP
                     FTSGDILQVLFVAVLFGISLAIVGERAKPVTAFLQAVSLPVFKLVSILMRVAPIGAFG
                     AMAFTIGKYGIASVINLAMLVGTFYLTSILFVLIVLGAVGRYNGFSIVRLVRYIREEL
                     LLVLGTSSSEAALPTLMQKMEQAGCSKSVVGLVVPTGYSFNLDGTNIYMTMAALFIAQ
                     ACDIPLSLGDQILLLLVAMLSSKGAAGVTGAGFITLAATLSVVPSIPVAGMALILGVD
                     RFMSECRALTNLVGNAAATIVVARWEGELDKSQLDAALKGETA"
     gene            14097..14294
                     /locus_tag="VGC69_03560"
     CDS             14097..14294
                     /locus_tag="VGC69_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HEY0294395.1"
                     /translation="MDVSVSSMINMSMDMQSTAVTQDIGAIMLRKTLNVAAQQIATLM
                     QAAMPQLATSGTLGTVVNTYA"
     gene            14377..14862
                     /locus_tag="VGC69_03565"
     CDS             14377..14862
                     /locus_tag="VGC69_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HEY0294396.1"
                     /translation="MLKLRTAGLVLFSSTLLLGATAQAQLLNAVKGAVGASSSSDGAA
                     SSGSSLLGGLGGAAGGSALPSLSSVGTGNLTGVLTYCMQNKYLSGGEASGVQSQLLGK
                     LGGPAKAKSDPGYQQGISGILGGNSSQKMDLTGGGNSGIKQQITDKACDQVLQYSKSA
                     L"
     gene            complement(14887..15441)
                     /locus_tag="VGC69_03570"
     CDS             complement(14887..15441)
                     /locus_tag="VGC69_03570"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0294397.1"
                     /translation="MTLQAMPQPNAYRLLEAGPVVLVSTAHRGRANIMTMGFHMMVQH
                     PSLIAAVIGPWDHSYQALKKTGECVIAIPTVDLAKKVVDIGNCSGSEVDKFERFGLTP
                     VAAQSVRAPLIAECVANIECRVADTSLVDRYNLFLLDAVRVWTDPDRKERRTLHHRGD
                     GTFAVDGRILDLRERMVLWKQFQD"
     gene            15530..16312
                     /locus_tag="VGC69_03575"
     CDS             15530..16312
                     /locus_tag="VGC69_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294398.1"
                     /translation="MKRLALLACACALALVQSLALAQSAPSTLGKVAQSGVLRVCSPG
                     DYKPFSFMKAPDEFEGLDVDLMGSLAAALGAKPQFIKTTWANLLPDFAAGKCDIAVGG
                     ISISLPRQKQVYFSDGYMVNGKTPITLCRNVAKYQSIAAIDKPTVRVITNPGGSNETF
                     AKTKLPHAKLIINPDNLSIFDEIIKGRADVFVTEAAEAIVQSRLHHELCPVNPAKPLQ
                     YAEMGYMLPSGDEVFKRFVDQWLHLAKASGEYDQLNSKWLGK"
     gene            complement(16327..18537)
                     /locus_tag="VGC69_03580"
     CDS             complement(16327..18537)
                     /locus_tag="VGC69_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0294399.1"
                     /translation="MDGVFADVESISFAALDRSALAVVLLDDTQSVVFFSRAAERLWG
                     YTREEMLGRDAMSWLPYFGGNFEDSGDGLGQARELLVERKDGRRGKALFIRSLVDIEG
                     KRLHMIAALARDDASGQDSEEQDRQRRIRDLERDCLEALTRNMSFDSLGKYLCHRIES
                     IVPGVRASICRVSDHRLKPWAAPSFPQDYCLAFDGVEIGEGVASCGTAAYRGEPVMVS
                     DLETDPLWAGYKHIVLPYGYRACWSYPIKRRDGSVAGTFAFYFKRSGWPSEYLARIAE
                     ASVHLCLLAFEREETRAQMDRLIKFDGLTGLPNRSHMDQHIDTLLDGDVGRGVALFMI
                     DLDRLKDVNSALGHGAGDLVLVEMADRLQGQLRPGQFLGRFGGDQFLLAARDCDADGA
                     VHIAGRLLEAVNGQIEIGGYTLNLSASVGISRCMAGKMESRSDLLGMVKTALLRVKAV
                     GGGTYQFFCRAMNVMVEDRLLLSSELKQAIAANQLTLHYQPQVRSDSGEIYGFEALAR
                     WHNERVGAIPPDRFIPMAEETGQIDALGFWALRETCRQLSQWRGAGLDVPVVSVNLSP
                     LNFRNPKLVAFLEGLLDEFGLRGSSLTIEITESTMLELTSEMLAAVHGIRALGLGLSV
                     DDFGTGFASLSNLINLPLTEIKIDRSFVELCPKESRQQALVETVIGIGRSLGLTVVAE
                     GVETPAQYGLLTEYHCPVLQGHFFAHPLDPADVPGWLNGHDLQTDGGIHPYYLG"
     gene            18674..19876
                     /locus_tag="VGC69_03585"
     CDS             18674..19876
                     /locus_tag="VGC69_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY0294400.1"
                     /translation="MTHKPTHDNNAALRHRDGHHARVTYEELFFDLVYVFAVTQLSHE
                     LLHHLNPMGLMETVILWFGVWLGWQYTCWVTNWFDPETPRIRGMLFVTMLLALLMSSS
                     IPAAFGDRGLIFASCYAAMQVGRTAFIVRELGRDHPLAANYRRMLVWLCLSAVFWIAG
                     GLLDHEARIALWIVAIACEYFSPMFGFVLPGLGRSSTRDWTVEGGHLTERCQLFVIVA
                     LGETLLACGAALTDAQAWNMDILSALGATFIGTLSMWWLYFGTSSKDATAVITSSTNP
                     GRIGANFHYIHAILVAGIIGSAVGNDLVFAHPHASPETAQVLALAGGPAVYLLGSAIY
                     KYVVYGRIPVSHVAGALALVALIPACYGSNLLVMGWLTTAVLLAVSFWEARMRRQPIL
                     GENQAHHP"
     gene            20110..21285
                     /locus_tag="VGC69_03590"
     CDS             20110..21285
                     /locus_tag="VGC69_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0294401.1"
                     /translation="MERLRFGICGLGFAGSVLMAPPMKQHPNAEIVAACDPNADIRAA
                     FGKDYGIATYDSLRAMLDDADLDAVYIASPHQYHCEQVVLAAERGKHIIVEKPLTLND
                     DDARRMVDAVARHQVQMVVGTSRSHDPVVKTMRDIVGSGELGRLAMINAWNYTDYLYR
                     PRRPEELDTSQGGGILFSQLPHQLDVIKTIANATIVSIRAGSGRLDPKRPTEGHCAAF
                     VTLSNGVFATLAYSGYDHFDSDELQFWIAESGREKRPNHGGARKLLAALAPEEEAAFK
                     TQRLGYGGPVARNYAATEASRKQPHFGIFVATCERGDLRPCPEGVLVYGDEGPRTVPA
                     HVGRGAYGQGDTIDELYDAVTGAGPAVRDATFGRNTVLACLAILESSRTGREVAVLS"
     gene            21346..22668
                     /locus_tag="VGC69_03595"
     CDS             21346..22668
                     /locus_tag="VGC69_03595"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0294402.1"
                     /translation="MTDHDDPYRILTAVGPGTPMGNLFRRFWLPALLSEELPAADCPP
                     VRIRILCEDLVAFRDSSGRVWIIDAYCPHRRAPLFFGRNEENGLRCVYHGWKFDVNGE
                     CVDIPNIIPPDNFAALKQRASIKGYPAQESGGIVWVYMGPKELMPELPGMEWVGLPDD
                     QVNCSRWLHRSNWLQAAEGEIDTSHISFLHNRVNVGPNSPDWQRLAQDGAPQITVRET
                     DYGFVYGSRRSFEGQYFWRVTQWMLPMWSAVPRSIGDPILAGRAWVPVDDYTTTGFGY
                     LYAADRPLSTKEREEVASGERFPPRARKGTFRLPHGYVIDTFLPDANIGNDFLIDRQI
                     QATTNFTGIFGVNEQDRALQESMLSIPGHVGIVDRSKERLVKSDLPIMTARRMLTKLA
                     KDLIQGIEPKAASSADQYGVRGIAILSPIADFDEFMRVHANSMEAAEA"
     gene            22670..23638
                     /locus_tag="VGC69_03600"
     CDS             22670..23638
                     /locus_tag="VGC69_03600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY0294403.1"
                     /translation="MAINGDSHSHIEARLHSITQEAEDILLFDFRPGLGQHFPGVEAG
                     AHIDLHLANGLSRSYSVINSQDDRDRYLIAVKKEAAGRGGSRFIHDELRVGQRIAIGG
                     PRNNFRLREASAHSVFIAGGIGITPLYSMIQRLERLERSWELHYAAAHREQAAFVEPL
                     ATLQPKTHFYFKSDQAGQGALDLRQIIRDSRPDADFYCCGPTRMLRDFEAATASIPPD
                     RVHAEYFTALEAPAAEGGFEVVLQRSGQRLAIQAGETILDALIKLGTDVPYSCMEGVC
                     GSCQVRVIEGTPDHRDLILSKQERDMNDRMLICCSGSKSAKLVLDL"
     gene            complement(23671..24585)
                     /locus_tag="VGC69_03605"
     CDS             complement(23671..24585)
                     /locus_tag="VGC69_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0294404.1"
                     /translation="MVDPLAEVVTLLQPGARFSKLVFGASPWRISRSDAGQPFYCVML
                     EGGCRVTIDGHEPIELLSGDFVLIPAAYGVEMSSLEPPPPGVETPAPVALGNGEFRIG
                     GPDSPIDARMMAGHCSFGSPDASLLVSLLPQFVHVRGERRLATLVQLVREESREQRPA
                     REVVLSRLLEVLLIEALRSTAGTNASPGLARGLSDCRLAAVLRGMHGRPTHAWTVVEL
                     AKEAALSRSAFFERFNRAVGVTPMEYLLAWRMALAKDLLRRNAGRVAEIAERVGYRSA
                     STFSVAFTRHVGRPPTQYAREQAAANAA"
     gene            24687..25469
                     /locus_tag="VGC69_03610"
     CDS             24687..25469
                     /locus_tag="VGC69_03610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0294405.1"
                     /translation="MKTVLITGCSSGFGLEIARYFLARDWRVVATMRTPRDDVLPHSD
                     RLRVLALDVTDPESIRKAVEASGTIDVLVNNAGIGAAAPAELTPMATVREIFEANTFG
                     TMAMTQAVIPQFRQRQTGVVVNVTSSVTLRALPLLAAYSASKAAVNAYTESMALELEP
                     FGIRARLVLPGRSPETRFGENARTHMHGFDHPAYAGLVQKVFAALTDTSSPVTYAQDV
                     AEAVWRAATDPSSPMRIPAGADAQAWAAEARRLAGDNTRVFD"
     gene            25498..26064
                     /locus_tag="VGC69_03615"
     CDS             25498..26064
                     /locus_tag="VGC69_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0294406.1"
                     /translation="MPRPKEFDRDEALDASIGVFREHGFAGTSAGMLTDAMKIGRQSL
                     YDTFGDKWRLYCAAVQRYAVAEAQAHFAALKTGPKAMDGICRMMERVVAEARMPCLGV
                     SSISEFGDSRKDLAQLRNAAGRALRAAIADKVRQAQAEGDAAVKLDPGHAAGFLIANV
                     AAIRLAARAGAGDAELRALGRLAITALR"
     gene            26113..27081
                     /locus_tag="VGC69_03620"
     CDS             26113..27081
                     /locus_tag="VGC69_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY0294407.1"
                     /translation="MKAIVMTGTGGTETLRHVERPDPAPGAGQALVEVAAAGVNFMDI
                     GVRQGMAWRDVPNPKILGVEGAGRIVAVGEGVAGFTPKQRVAWVYAPGSYAEKVVIPA
                     TALVPIPDEVDDRTAASLMMQGLTASHFGTDFHPVQPGEFALIHAAAGGVGLLLTQII
                     KMRGGHVIGRVSSAAKVAVAKVAGADHVIVDAEGRFAEEAIRLAGGEGVHVVYDGSGP
                     KTFQGSLDALRRSGTFCWYGPVLGGPGPIDIMSLPRSIKIGYATFMDHIHTPELLRAR
                     SARLFDWVIEGKLKIRIGGVYALADAARAHADMESRATTGKLLLIP"
     gene            27181..28566
                     /locus_tag="VGC69_03625"
     CDS             27181..28566
                     /locus_tag="VGC69_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEY0294408.1"
                     /translation="MRRTILFLQGPPSLFWRQLSEHFEASGAKTHRINLSLGDWAYWR
                     KRGAVNYRGRFSRWADFLRDYLVRHQVTDILYYADRLPYHAVAREVARSLGIRTYAVE
                     FGYLRPDWLTLERGGMGTLSHFPDDPAAIRAIARRAGPFRPETAHYSHTFGQEAFNEV
                     FYNLLTSLVPYFFPLYRTDKYYLPLRDYLSWLPRMVSQRLREPARVAAADAMRHSNKR
                     YWMLALQMQADYQLRVNSAYDHQGRMIEEVIASFAQHAPRDGALIVKLHPLDNGIERW
                     EKFVTRTAARHGVAARVVAVTECDLKRTIKRSQGVITVNSTVGLHALRANCPVKVLGM
                     AVYDIAGLTHQEPLDTFWSKPQRVNKALLKDFVSAIAATIQVHGSFYNPAGRKAACAE
                     IVRRVLNDQVNALGAFIDPPPRLGRALQAEVPIFSPLRDSGVEPVLTAGTATPHETAG
                     QPSAANSAPAV"
     gene            complement(28508..30574)
                     /locus_tag="VGC69_03630"
     CDS             complement(28508..30574)
                     /locus_tag="VGC69_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEY0294409.1"
                     /translation="MMFSTDFPTHGDEVGNEQSRQRARAVPPTLAYTLAPGVRGIPTL
                     AALLDDFQLRYRPVGRPAGNAGVLAWGRRPSAIRAQQYAQRRGLAVYHIEDGFLRSVG
                     LGKDDPPLAIIFDDLGLYLDASRPSRLEVLIGQPLDGDQSARARALRSAWCDGRVSKY
                     NHSRDLARALPGDCVLVVDQTRGDASVACGLADAASFERMLAAALREHPDSTILLKVH
                     PDVVSGRKQGHFDLDAVRAMPRVQVLDDDVHPSSVLSAVRAAYTVTSQLGFEALLWGL
                     PVRVFGMPFYAGWGLTQDELPAPARRGAATLEQLVHAALIDYCRYVDPETGKRCEVET
                     LLAWLALQRRMRQRFDLKIDALGFSGWKKGYVRQYFGGSAVRFRWGVPRSTRGAVATW
                     GRKLDGRLASNPSGTGQVIRVEDGFIRSVGLGADLTRPLSWVQDDVGIYYDATGASRL
                     EQLLAQTAFTPALVARAASLREAICDAGITKYNLADAAHWVRPPDKCVILVPGQVETD
                     ASIRYGASAIRGNMALLQAVRAANPDAYILYKPHPDVMAGLRKVGRDENSAARWCDEI
                     IGAASFHQLLGSVDEVHVLTSLAGFEALLRNVKVVTYGQPFYAGWGLTRDVELTDALR
                     RRRARALTLDELVAAALILYPTYVSRDTGRFTTPERALRELIEWRARPRVPSWRHWVA
                     RLFREA"
     gene            complement(30558..>30755)
                     /locus_tag="VGC69_03635"
     CDS             complement(30558..>30755)
                     /locus_tag="VGC69_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294410.1"
                     /translation="SPGKSDVLKRYSLGAVQCHRPECMGMATAAGGDIRARGELERWL
                     ATVNNGVGVIQDSFRADDVFN"
BASE COUNT         5559 a        10852 c         9422 g         4922 t
ORIGIN      
        1 aagacgttcc tgggcggtgg gcataccccg cggcttcgct gtgctgggct cgggcgcaac
       61 caggcgcccg agcccggcac agcgaagccg ccagggcctc tccgccgaac gcaggacgtc
      121 tcaaaaactc aaaacaacgg cctacgaaaa agagcaggcc gaggcgaccg gacagcgagc
      181 gattaccgcc gcccgcccag caaggacccc agcacccccc gcaccaactg cgtggtcacc
      241 tggcgcgcca ggctcttggc cgccacctgc accaggccgt cgtgctgccc gccgcgcggg
      301 cccacatggc cgaacagcac gttgctgacg ccccccatca acccgccgcc accgccctgc
      361 tgcgcgggcg cggcgggctg gcccgctgcc ggcggaaccg gcgcggcgcc cgttccgctg
      421 tccggcacgg acgccgtcgc ccggcccgtc agccgttcat aggccgactc gcggtcgacg
      481 gtctgctcgt acttgcccgc cagcgggctg ccctggcgca gcgcctgccg ctcggccggc
      541 gtgatcgggc cgatgcgccc gcccggcggc atgatccagg ctctctccgt cggggtggga
      601 atgcccttgt cgtcgaggaa ggaaaccagg gcctcgccca cgcccagttc gccaatggcg
      661 gcctggatgt ccagcccggg attgggccgc atggtgtccg ccgccgtctt caccgcctgc
      721 tggtcgcgcg gcgtgaaggc ccgcagcgca tgctgcaccc ggttgcccag ctggcccagc
      781 acgcggtcgg gaatatccag cgggttctgc gtggcgaaat acacccccac gcccttggag
      841 cgcaccaggc gcaccacctg ctcgatcttc tgcatgagcg ccgacggcgc ctcgtcgaac
      901 agcaggtgcg cctcgtcgaa gaaaaacacc agcttgggct ggggcaggtc gcccacctcg
      961 ggcagttttt cgtagagatc ggccagcagc cacagcagga acacgccgta caggcgcggc
     1021 gcctgcatca gcttgtcggc cgccagcaca ttgacgatgc cgcgcccctg ggcgtcggtg
     1081 cccagcaggt cggccacgtc cagcatgggc tcgccgaaga acttgtcggc gccctgcgac
     1141 tcgagctgca gcaggctgcg ctggatggcg cccacgctgg cgtcggacac attgccgtag
     1201 cgggtcttca gctcggcggc gcggtcggcc acgttctgca gcatggcgcg caggtccttc
     1261 aggtcgagca gcagttggcc ttcgtcgtcg gccacgcgga acaccagggc cagcacgccc
     1321 tcctgcacgt cgttgagctc cagcatgcgc gccagcagca gcggccccat gtcggacacg
     1381 gtggcgcgca ccggcaggcc ctgctcgccg aacacgtccc acagcgccac cgggcaggcg
     1441 ccccaggcgg gttcgggcag gcccaggcgg gccaggcgct cctgcagctt gggagacccc
     1501 gtcccggcct gggaaatgcc ggtcaggtcg cccttgacgt cggccatgaa gaccggcgtg
     1561 cccatgcgcg aaaaggcctc ggccaggact tgcagggtca ccgtcttgcc ggtgccggtg
     1621 gcgccggtga tgcagccgtg gcggttggcc agggcgggca gcagcgccag ttcggcatgg
     1681 gcatttttgg cgacgacgat gggattgggc atggaatcgg tctccgtacg gctggcagat
     1741 acctgacagt ttagcgagta ccagtcgccg ccggtcgata cgcccatttc gcgccggcgc
     1801 ccaccgcctt ccccggcaag ctaaaatagg ccatttgccg aatcacctaa gagaaaaaac
     1861 atggccggac acagtaaatg ggccaatatc cagcaccgca aagggcgtca agacgccaag
     1921 cggggaaagc tctggacgaa gatcatccgt gaaatcacgg tagccgcccg cgccggcggc
     1981 gccgacccgg acgccaaccc ccgcctgcgc atggcctggg acaaggcgac cgacgccaac
     2041 atgcccaagg acaacgtcca gcgcgccatc gcccgcggcg cgggcggcgc cgacggcgcc
     2101 aactacgaag aagcgcgcta cgaaggctac ggcgtgggcg gcgcggccgt catcgtcgac
     2161 tgcaccaccg acaaccgcac ccgcacggcg gccgaagtgc gccacgcctt caccaagaac
     2221 ggcggcaacc tgggcacgcc cggctcggtc gagttcatgt tcaagcactg cggccagttc
     2281 cttttcgcgc ccggcaactc cgaagacaaa gtcatggaag ccgcgctcga ggcgggcgcc
     2341 gaagacgtca tcaccgacga cgaaggcggc atcgaggtca tctgcgcgcc cggcgactat
     2401 cctgccgtca agcaggcctt gggcgccgcc ggcctcaagg ccgaggtcga cggcgtcatc
     2461 atgaaggcgc tcaacgaaac cgagctcacc ggcgacgacg ccatcaagat gcaaaagctg
     2521 ctcgacgccc tggaagacct ggacgacgtg caagaggtct ataccaacgt cgtgttcgac
     2581 gaggcgcaat gaaactcctg gttatcggtt cgggcggccg tgaacacgcc ttggcttggc
     2641 gcctggccca atcgcctcgc gtgcaccggg tctacgtggc gcccggcaac ggcgggaccg
     2701 gcctcgactc gctgctcgac aacgccccct acaccacggc cgaagagctg gccgatttcg
     2761 tcgaacgcga agacatcggc atcaccgtgg tcggccccga ggcgcccctg gcggccggcg
     2821 tggtcgacgt ctttcgcgcc cgcggcctga agatcttcgg cccgaccaag gcggccgccc
     2881 agctcgaaag ctcgaaggac tacgccaagg ccttcatgat gcgccacaag atccccacgg
     2941 cgcgctacca gaccttcacc gacccggccc aggcccacgc ctacatcgac caggaaggcg
     3001 ccccgatcgt catcaaggcc gacggcctgg cggccggcaa gggcgtggtc gtggccatga
     3061 ccctcgaaga agcgcacgcc gcggtcgacg gcatgctgag cggcggcacc atgggtatcg
     3121 cgggcgcccg cgtcgtcatc gaggaatacc tcgccggcga agaagccagc ttcatcgtca
     3181 tgagcgacgg ccgcaacgtg ctggccctgg ccaccagcca ggaccacaag cgcctgctcg
     3241 acggcgatgc cggccccaac accggcggca tgggcgccta ctcgcccgcc cccatcgtca
     3301 cgcccgagct gcaccaccgc atcctcaagg aaatcatcct gcccaccgtg cacggcatgg
     3361 cgctggacgg catcccctac accggttttc tctatgccgg cctgatgatc ggccccggca
     3421 gcgatgccac gcgcttggtc aagaccatcg agttcaactg ccgcctgggc gatccggaaa
     3481 cccagcccat catgatgcgc gtcaagagcg acctgctcga cgccatcgag cacgccatcg
     3541 acggcacgct cgaccaggcc gacctcgcct gggaccgccg cacggcgctg ggcgtggtca
     3601 tggccgcgca caactacccg ggcacgccgc gctcgggcga cgccatcacg ggcctgcccg
     3661 ctccggccga agactgcatg gtcttccacg cgggcaccac gctggatggt ggtacgctca
     3721 agacatccgg cggccgcgtg ctctgcgtca cggccctcgg cgactcggtg cgcatggcac
     3781 aggatcgcgc ctataaagcg gcgtcagcca tcaatttcga cggtgcccag taccgccgcg
     3841 acatcggctg gcgcgccctc aaaccctcgc agcagcgcaa gccgcaaccc tgacctatga
     3901 gcaccacgcc ttgcgcgatg gatgcggccc ccgtccgcga ttacctgacc aacctgcaag
     3961 cccgcatcgt cgccgcgctt gaaaaagccg gcggagacac tttccgcacc gatgcctggc
     4021 agcgccccga gggcggcggc ggcctgtcgc gcctgatcga gggcggccag ctgctcgagc
     4081 gcggcgccgt gctgttctcg catgtcaccg gcaccctcct gccgccctcg gccagcgccc
     4141 accggcccga tctggccggc cgcgcctggg aggccatggg cgtgtccatg gtgctgcacc
     4201 cgcgcaaccc ctacgtgccc accacccaca tgaacgtgcg catgttccgc gccgtggcgc
     4261 ggccgggata cgacgagcgc gaggtgttct ggttcggcgg cgggctggat ctcaccccct
     4321 actacccgtt cgaggcggac gcctgccatt tccacggcgt ctgccgcgac gcgctgtcgc
     4381 ccttcgggcc cgattactac ccgcactaca agcgctggtg cgacgagtat ttcttcctca
     4441 agcaccgcaa cgaagcgcgc ggcatcggcg gcatcttcta cgacgacctc aacgaacccg
     4501 acttcgacac ggcgttcgcg ctcacccgct cggtgggcga cgcgtttctc ccggcctatc
     4561 tgcccatcgt cgagcgccga tgtaagaccc cgtacggcca gcgcgagcgc gacttccagt
     4621 cctatcggcg cggccgctat gtcgagttca acctggtcca cgaccgcggc acgctgttcg
     4681 gcctgcagtc gggcggccgc accgagtcca tcctgctatc catgcctccc atcgccaact
     4741 ggcgctacga ctggcacccc gagcccggct cgcccgaagc ggcgctgcag gactttctcg
     4801 taccgcgaga ctgggtctga acatggccaa gcgcatcggc ctgctcggcg gcagcttcga
     4861 ccccgtgcac gaggcgcaca tcgccctggc actggcggcc aggcaggcgc tggagctcga
     4921 cgaggtccag ctcatccccg ccgccatgcc ctggcaacgc ggcgacctga aggccgccgc
     4981 caagcaccgc ctgcgcatgc tcgacatcgt cgcggccaag catcccggcc tgacggtcaa
     5041 ccccatcgaa ctcgagcgcg gcggcaccac ctataccgtg gacaccctgc gcgccctgcc
     5101 cgccggcccg gactacatct ggctgctggg cgccgaccag ctggccaatt tctgcacctg
     5161 gcgcaactgg cgcgaaatca ccgaacgcgt cgacctggcc gtcgccgtgc ggccgggcac
     5221 gcccctgcat gcgccggacg aattggccga ctggcttgcc acccgccaga gccctctgca
     5281 acagcttccc ttccaaccca tggatgtctc ggcttcggac atccgtaccc gattgaccag
     5341 cggcgcttcc accgccggcc tgctcgaccc cgacgtggcc acctacatcg ccacgcataa
     5401 actctacgtg tagggtaatc gaccccatgg acatacaaaa actacaacgc gccgtcatcg
     5461 atgccctcga agacgtcaag gcccaagaca tcaaagtctt caacaccatg catctcacca
     5521 gcctgttcga ccgcgtggtg ctggccagcg ccacctccaa ccggcagacc cgggcgcttg
     5581 ccgccagcgt cgccaaccgc ggccgcgaac tcaagctcac cggcatcacc atcgagggcg
     5641 aggacactgg cgaatgggtg gtggtcgacc tgggcgacat cgtcatccac atcatgcagc
     5701 ccgccatccg ccagtactac aacctggaag agatctgggg cgacaaaccg gtgcgcgtga
     5761 agctgctgcc cgagtccacc cgcccggcga tgggcagcga agcaagcttt taaggtccag
     5821 gccagcctgt acatgagccg aggaccccgg aggaatccga agcgcagccc ccaccggagg
     5881 cgagcatcgg aattccgcag ggcagcctcg gcgcctcaag aactcagata ccccccaccg
     5941 tgaagctcat catcctcgcc gtcggcaacc gcatgccgga ctgggtgcag accgcctggg
     6001 acgactatgc caagcgcctg cccgccgact gcgcgctcga gctgcgcgaa atcaagcccg
     6061 aaccccgcac ctcgggcaag accccggccc agatgatggc cgccgaagcc aagcgcatcg
     6121 aggccgccct gccggccggc gccctgcgcc tggccctgga cgaacggggc cgcgacctga
     6181 ccaccatggc gctttccaag caactggaac aatggcgcgc cgacggccgc gacgtggcct
     6241 tgctggtggg cggccccgac ggcctggacg ccgcgctcaa ggcctcctgc gacggccagt
     6301 tgcggctctc ctcgctcacg ctgccgcacc ccatggtgcg cgtgctgctg gccgaacagc
     6361 tctaccgcgc ctgggccatc atgaccaacc atccctatca ccgggcatga gcacgccgag
     6421 ccgcctcaag tgctcattcc cccttggggg gacggcgcgc agcgccaagg ggacaatatg
     6481 agcatgcctc gtctctacct ggcctcggcc agcccgcgcc gccgcgaact gctgctgcag
     6541 atcggcctgg cgcacgaagt cctgcgcgtg ccggctccgc ccggcgacga cgagccgcaa
     6601 ctgcccggcg aaagcccggc cggctacgtc ctgcgcaccg cccgtgaaaa agcccagcgc
     6661 ggcgaacaat ggatgcggag ccgggccctg cccgccctgc ccgtgctggc ggccgacacc
     6721 accgtggtcc tcgacggcgc ggtgctgggc aagccggccg accgggacga cgccctgcgc
     6781 atcctgcagg ccctctcggg caccacgcac gaagtgcgca ccgccatcgt cctggccgtc
     6841 cagggccgcc tgctcgaagc ggtttccgtc acccgggtcg ccatgcgcgc caccagcgcc
     6901 gccgaacggg aacgctattg cgacagcggc gagccttacg gcaaggccgg cgcctacggc
     6961 atccagggcc tggccagcct cttcatcgaa catatcgcgg gcagctacag cggcgtgatg
     7021 ggcctgcccc tgttcgaaac cgcccggctg ctggctcagg ccggcatcga gctgccctga
     7081 gccggcccag gcctggcgcc gccaggcagc acggcccaca gcgacacgcc caccatcagc
     7141 gccccgccca gccaggccag gggcgtcagg cgctcaccca gccagaggca ggcgaccagg
     7201 gcgccgaagg cgggttcgct acccatcagc aggctcaccc gggtgggatg gctgcgctgc
     7261 atggcaaagc tctgcacaaa gaaggcaaac accgtgcaca ccagggccag gtaggccatg
     7321 ccgacccaga acccgtcctg cgacggccac gccggcaagg gcccccattg ccccgccgcc
     7381 aaggccgcga tagcgccgcc ccctgccacc acgcccgact gcaccgcggt cagcatcgtc
     7441 ggcgtcagcc gggcatcgcg catcacccgt ttcatcacgc atccatgcag cgcccgcagc
     7501 aaggccgcca gcaggatcag ggcatcgccg gcctgcagcc tcgcgccggc gccgctcagc
     7561 agccaggcgc cgaccaggga cagcgcggcg gccacccact ccttgcgggc cggccgccga
     7621 tgcagcaagg cccattcggc ccagggcgtc agcacgacgc aaaggctgat caggaaagcc
     7681 gccgtcgacg cttcggtcag catcaccccg aaggtttccg ccaggaaaat gccgaacaat
     7741 acggcgccgc accccagggc gccccacacg gacgaacgcg gcaaggcccg caggccccgc
     7801 agcgccggca gcagcagcac gaacgccagg ccgaagcgca gcgccagcac gcccagcacc
     7861 gaataaaaga ccagcgtgct tttcacgacg gcatacgtgc tgccccagac ggccgccacg
     7921 gccagcagca gcgcatcgga caggcgcagc caccctgctt ggttcttcat gacgaggatc
     7981 cctttgatgg aaatacccgt catttttcat tagtgccgat aaaatgataa ttacccttta
     8041 gtgcaaaata tttatgcaaa atagacatag ataatcccgc ccatccgcca tgcaccccga
     8101 cacgctctcc gccctgctgc ccgacctggc caccttcgcc cgcgtcgtcg atgccggcaa
     8161 tttttccgag gcggcgcgcc agctgggcag cacgccctcc acgatcagcc ggcagatcaa
     8221 gcggctggaa accgccctgg gcacgcgcct gctggagcgc tccacccgca gcgtgcgcgt
     8281 caccgaatcc ggtgagcagg tctaccggcg ctgccgcgac atgctcgagg ccgccaccag
     8341 cgccatcgac gcggccagcc ggctcgccgc gcaaacgcgc gggctggtcc gcttcagcgc
     8401 gcccatctcg tacgcgcact acgtcatcca cccgctgatg ccggccttcc tgcgcgccca
     8461 cgaggccgtg gacgtgcagc tcatcttcac cgaccgggaa ctcgacccct tgcaggacga
     8521 cgtggacctg gtcatccgcc tgacccgcca tcccccgccc gggctcgccg cgcggccgct
     8581 gggcgaggtg cgctggatac tctgcgcctc acccgggtat ctccaggcgc gcggcgcccc
     8641 tcggcagccc gccgacctgg cggcgcatga ctgcatctac ctgggcgaga cgccccagga
     8701 caggcactgg accctgcatc gcggcaatac ggtgcaaacc gtggacgtcc ggggccgcta
     8761 tcaggccaac cacgccgatg cccggctgga tgccgccctg cggcatctgg gcatcgcctc
     8821 cctgcccgaa ttcctggccg ccgaggcctt gcgcagcggc gcgctggccc gcgtcctgcc
     8881 cgactgggat ttcgagcccg ccgcttatac tgggccagtc tggctgctgt acccgcccaa
     8941 ccgcttcctg ccgcccaagg tacgcgccct gatcgaccac ttggccgccc acttgcccgg
     9001 gaaccaggcg taaccgctgg ccaagggcct tcgccccctg tccatgaaaa tgtgaaaaaa
     9061 taaaacagga aaaacgttgc aggctcaaat ggcacgctgg gtatagttgc accgcgcaac
     9121 aattatctac ttaacgtttt tttatttctt cgcccttcac ggcaccctat cccataggtt
     9181 atggagcaaa cacggtccgc cacaccagcg cagccggcat ctttgccggc tggctcgttg
     9241 tcggctgtcg acactccacg ctggcaccgt tccttattgg gcaagaccat cctcttcgtg
     9301 ctgggggccg tgctgctggc ctacggcatc ggcgccggcg tcggcttcat gatggtggaa
     9361 cgcatctcgc gcgagcaatg gtggagccag gccgccacct atacccagat ctccgccacc
     9421 gccctgcgca gcatctacac cgccatctcc gtcgaaacca acgccgacgg gcagatcacc
     9481 cgcatcgtgt ccgaaagccc catcggcgac gcggactcca tcctgcaaac cggcttcagt
     9541 ccggtcgacg tcctggcgct ggcggcggcg cagacccgcc acaatgtctg gctgttccgc
     9601 tgccaggaag gccacgcatc caatatgtcc atggtcagcg ccctcgacgg cacgcagggc
     9661 gcccccgcgg ccggcgacgg ccagacctcc ctcacccccg acgagatcaa gaacccctat
     9721 gtgggctttg cccgcatcaa tggcgccgaa tacttcgtca gcgccatccc catcgccacc
     9781 catgccggca agcagacggg cgtgctcatc tccagcatcg gcctcacgca gtcgctctac
     9841 cagacgcgca acgcgctcat ccgcaactcc ctgctggtgc tgctgggcgt gctgctggcg
     9901 gccaccgtcg ccatcatctg gctcatgcac cgcctgttcc ggcccgtgcc ggcgctgatc
     9961 cagtcgctca tgcgcatcac gcacggcgac atccagtccg tcacgcccta ccagggccgg
    10021 cctgatgaaa tcggccggct ggccaccgcc atcgaaaccc tgcgcaacgc cgtcgccgag
    10081 cgcgaacagc tgctgtccat ccaggaagcc gccatgcggc tcgagcacat ggcgcaccac
    10141 gatgcgctga ccggcctgcc caatcgcgcc ttcctcgacc gcaccctgtc gcatgccgtc
    10201 gccaccctgc cctccggcgc ccgcttcaat gtgatgctgt tcgacctcga ccgcttcaag
    10261 gaggtcaacg acagctacgg acatgccatc ggcgatgcgc tgctcgtggc catcagcgac
    10321 cgcctgaacc tgctgctcgg ccccgaggac gtcgccgcgc ggctgggcgg cgacgagttc
    10381 gtggtggtgc agaaggtcgc ccgcgatgcc ctgctggaag ccagcaagct ggcctcgcgt
    10441 atcatcgaga cgctcaagcg cccgttcctg gtcgacgggc gggaactgca catcggcgcc
    10501 agcgtcggca tcacctgcgc gccgagcgac ggcgacaatg cccacgactt gctgaaaaac
    10561 gccgatactg ccctgtacgc cgccaagaac ggcggccgcg gccggttcac tttctacgaa
    10621 tcccgcatgt ccatggatgc acggacgcac acccccttgt cctagtagcc ctgttccaag
    10681 gcacgactat gcacctcacg cggatcatgg cggcctggcc gcgcaagcgc ctttgcagct
    10741 ggcagcgccg gctgcaaagc caggccgcct tcgccgccct ggccatcctg cccgtcgcgc
    10801 agcaggccca ggcgaccgac accctggcca agatcgccag cacccaccgc atcatcatcg
    10861 gccatcgcca gagcgagctg ccgttctcct acgtggcgaa cggcaaggtc atcggctatg
    10921 ccatcgacat ctgccagaag gtcgtcgaag gcctgcaaaa gcagctcaac ctggaccaca
    10981 tcgaaatcga ctaccagctg gtggccacgg ccacccgttt catccagctc aatagcggca
    11041 atatcgacct ggagtgcgcc accaccacca acaacgccga gcgccgcaag atcgccgagt
    11101 tttcctatcc ttatttcttc acggcgacgc gctacgtcgc gaagaagcag gcaggcctgc
    11161 acaccattgc cgacctggcc gggcacagcg tggtctccac caccggcacc atcgacgtcg
    11221 atcagctcaa cgccgtcaac ctggcgcagc atctcaatat ctccgtgctg ctgcggcgca
    11281 cgcacagcga ctcgttccag ctggtcgaaa acggccaggc ctcggctttc gtcatggacg
    11341 atatcctgct ggccggcctg gtggcctctt ccgggcaccc cggcgactac accatctcgc
    11401 aggacgcctt caaccggccc gaaccctatg gcatcctgat tccggccggc gacatcgcct
    11461 tcaagagcgc ggtcgaccgg gtcttgcgca cgctgtacgc cagccacgaa atcgaccagc
    11521 tctatgccaa gtggttcatg cagcccatcg cgccggacgg ccgcaacctc aagctgccca
    11581 tgtcgcccga actgcgcgac tggctggccg cgccgcgcga atacctggac tgaccggagc
    11641 gaccgccatg cgcctgcctt cgccttcccg acggttctgc ctgggccttg cggccgcgct
    11701 gctgaccgcc ggcctctcgc acgcccagac gccccctgcc tccacgctgg agcgcatccg
    11761 ccagaccggc gtgctgcgca tcggcttcgg ctacgcccag ccctttgcct atatcgaggc
    11821 caacggcacg gtcacgggct attcgatcga gctatgcggc cggctggccg agacgctgcg
    11881 cgtacaactg gccctgccca ggctggacat ccagtacagc cggctcgccc cgaacaaccg
    11941 catctcccag ctcaacagcg gcgcgatcga catcgaatgc ggcgccagca ccaactcgcc
    12001 ggaacgacgc gccagcgtca ccttcggccc cagccatttc atcaccgact cgcgttatgt
    12061 gtcgctggcg gccaacgcgc tgcgcacgat cgacgatctg cgcggcaaga gcgtggcagt
    12121 cgtgctcggc acgaccaacg tgggcgacat cgcccggctc aaccgcgagc gcaggctcaa
    12181 tctgtcggtc gtcacgacgc ataccaacca ggacgccttc gacctcatca cgcagggcaa
    12241 ggtcagcgcc tttcccatgg acgatgtgct gctgcacatg ctggtcggcg cttccggcaa
    12301 tcccgaccgc tatgccgtgt ccgacgagac cctggcgccg ccgcaggcct atggcttcat
    12361 gatgcggcgc aacgacccgg acttcgtcca ggccgtcgac gcggccctgc gcaatatcta
    12421 cgcgagcccg gacatgccgg ccctgtacga ccgctggttc aatgagccgc tgccgggcag
    12481 cggcgtcaat ctgcacctgc ccatgagccc gacattgcgc cgggcgctct tcggggccga
    12541 tgccggctga atgaaccggc atcgggcacg tccgactagg ccgtctcgcc cttgagggcg
    12601 gcatcgagct gcgacttgtc cagctcgcct tcccagcggg ccaccacgat ggtcgccgcc
    12661 gcgttgccca ccaggttggt cagcgcgcgg cactcggaca tgaagcggtc cacgcccagg
    12721 atgagcgcca tgccggccac ggggatggac ggcaccaccg acagcgtggc cgccagcgtg
    12781 atgaaacccg cgcccgtcac gcccgccgcg cccttggagc tcagcatggc caccagcagc
    12841 agcaggatct ggtcgcccag cgacagggga atgtcgcagg cctgggcgat gaacagcgcg
    12901 gccatggtca tgtagatatt ggtgccgtcc aggttgaagg aataccccgt gggcacgacc
    12961 aggcccacca ccgacttgga acagcctgcc tgttccatct tctgcatcag cgtgggcagg
    13021 gccgcttccg acgagctggt gcccagcacc agcagcagct cttcgcggat gtagcgcacc
    13081 agcctgacga tggagaagcc gttgtagcgg cccacggcgc ccagcacgat gagcacgaac
    13141 agaatggacg tgaggtagaa cgtgcccacc agcatggcca ggttgatcac cgaggcgatg
    13201 ccgtacttgc cgatggtgaa ggccatggcg ccgaaggcgc cgatgggcgc cacgcgcatc
    13261 aggatggaca ccagcttgaa caccggcagc gacacggcct gcagaaaggc cgtcaccggc
    13321 ttggcgcgct cgcccacgat ggccagcgaa atgccgaaga gcaccgccac gaacaacacc
    13381 tgcaggatgt cgcccgacgt gaaggggctg agcagcgtgg tggggatgat gttgaggatg
    13441 aagcccgaca ccgtggagtc gtgcgccttg gacacgtagc ccttgacctg gttctggtcg
    13501 agcgaggcgg ggttgatgtg catgccgccg cccggctgga tgaggtggct gacgatcatg
    13561 ccgatgacga gcgcgatggt cgagaacacg atgaagtaga tcatcgcctt gcccaccacg
    13621 cggccgacct tcttgaggtc gctcatggcg gcgatgccgg tggtcaccgt caggaagatc
    13681 accggcgcga tgatcatctt caccagcttg atgaagccgt cgcccagcgg ctgcatggcc
    13741 acggccgtat cgggcacata gtggcccagc gcgatgccga tgagaatggc ggcgatcacc
    13801 tgcacataca ggatttgata gaacttgggc ttgcgagcgg gtgcggaggg ttgctgcgtg
    13861 ttggcttgat ctagatcgat cattttttgt ctcgtgagta tgtcgacatg gcaaacctta
    13921 acaaaggctt agcgccgcat tctactatct ggcgccaagc cctggcccgc ttgccagagg
    13981 cctcgcccca atcgcaaaga agcgcaaaaa aagccgcctt ttcgccaagt tgttacaggt
    14041 tctgtcatct ggcgtgccta tgattccaca tccgccacgc tttgaaggaa atccggatgg
    14101 atgtctccgt ctccagcatg atcaatatgt ccatggacat gcaatccacg gccgtcaccc
    14161 aggacatcgg cgcgatcatg ctccgcaaga ccctgaacgt ggcagcccag caaatagcca
    14221 cgctcatgca agcggccatg ccgcagctgg ccacctcggg cacgctgggc acggtggtca
    14281 atacttacgc ctgatctgag acctcttgat accgcttgcg cagcgggccg gcgatacact
    14341 cgcctcgctt ggttcctcat ttcggagaca cacatcatgc tgaagctgcg cactgccggt
    14401 cttgtcctgt tttcttccac cttgctgctg ggcgccacgg cccaggcgca gctgctcaac
    14461 gccgtcaagg gcgccgtggg cgcaagctcg tcgtcggacg gcgcggcctc ttcgggcagt
    14521 tccttgctgg gcggtctcgg tggtgcggcc ggcggttcgg ccctgccctc gctgagctct
    14581 gtcggcaccg gcaacctcac cggcgtgctg acctactgca tgcagaacaa atacctgagc
    14641 ggcggcgaag cctcgggcgt gcaaagccag ctgctcggca agctgggcgg cccggccaag
    14701 gccaagtcgg accccggcta ccagcagggc atcagcggca tcctgggcgg caacagcagc
    14761 cagaagatgg acctgaccgg cggcggcaac agcggcatca agcagcagat caccgacaag
    14821 gcctgcgacc aggtgctcca gtacagcaag tcggcgctat aagcctggcc ggcgccggcc
    14881 gcgccgtcaa tcctggaact gcttccacag caccattctt tcccgcaggt ccaggatgcg
    14941 gccgtcgacc gcgaacgtgc cgtcgccccg atggtgcagg gtgcggcgct ccttgcgatc
    15001 cgggtccgtc cagacccgca ccgcatccag caggaaaagg ttgtaccggt cgaccaggct
    15061 ggtatcggcg acgcggcact cgatattggc cacgcactcg gcgatcagcg gcgcccgcac
    15121 ggattgcgcc gcgaccggcg tcaggccgaa ccgctcgaac ttgtcgacct cgctgcccga
    15181 gcaattgccg atgtccacca ccttcttggc aagatccacg gtgggaatgg cgatgacgca
    15241 ttcgccggtc ttcttcaagg cctggtagct gtggtcccag ggaccgatga cggcggcgat
    15301 cagcgaagga tgctgcacca tcatgtgaaa acccatggtc atgatgttgg cgcgaccgcg
    15361 gtgcgcggtg ctcaccagca ccaccgggcc ggcctcgagc aggcgatagg cattgggctg
    15421 cggcatggct tgcaaggtca tcggggcttc ctttatccgt atgtgtcctt attctattga
    15481 gcgcggggcg gacgtaacat cgggtaccgt tttttcgaca ggatccgcca tgaaacgact
    15541 tgcccttctc gcctgcgcct gcgccctggc gctcgtccaa tccctggccc tggcccagtc
    15601 cgcgccgtcc accctgggca aggtcgcgca gagcggcgtg ctgcgcgtct gctcgccggg
    15661 cgactacaag cccttcagct tcatgaaggc ccccgacgaa ttcgaaggcc tggacgtcga
    15721 cctgatgggc tcgctggccg ccgccctggg cgccaagccg cagttcatca agaccacctg
    15781 ggccaatctg ctgcccgact tcgccgccgg caagtgcgac atcgcggtgg gcggcatctc
    15841 gatctcgctg ccgcgccaga agcaggtgta tttcagcgac ggctacatgg tcaacggcaa
    15901 gacgcccatc accctgtgca ggaacgtcgc caaataccag agcatcgccg ccatcgacaa
    15961 gcccacggtg cgcgtcatca ccaacccggg cggcagcaac gaaaccttcg ccaagaccaa
    16021 gctccctcac gccaagctca tcatcaatcc cgacaacctg agcatcttcg acgagatcat
    16081 caagggccgc gccgatgtct tcgtgaccga ggcggccgag gccatcgtgc aaagcaggct
    16141 gcatcacgaa ctctgccccg tcaatccggc caagccgctg cagtacgccg agatgggcta
    16201 tatgctgccc agcggggacg aggtgttcaa gcgctttgtc gatcagtggc tgcacctggc
    16261 caaggccagc ggcgaatacg accagctcaa cagcaagtgg ctgggcaagt aaggcagcag
    16321 gagaattcag cccaggtagt acggatggat gccgccgtcc gtctgcaagt catgcccgtt
    16381 cagccagccc ggcacgtcgg cgggatcgag cgggtgggcg aagaaatggc cctgcagcac
    16441 cggacagtgg tactccgtca gcaggccgta ttgcgccggg gtttccaccc cttcggccac
    16501 gacggtcagg cccaggctgc ggccgatgcc aatgaccgtc tccaccagcg cctgctggcg
    16561 gctctccttg gggcatagct cgacaaagct gcgatcgatc ttgatctcgg tgagcggcag
    16621 gttgatgaga ttggacaggc tggcaaagcc cgtgccgaaa tcgtccaccg acaggcccag
    16681 gcccagcgcc cggatgccat gcacggcggc cagcatctcg gaggtcagct ccagcatggt
    16741 gctctcggtg atctcgatcg tcaggctgct gccccgcagg ccgaactcgt ccagcaagcc
    16801 ctccaggaac gcaaccagct tcggattgcg gaaattgagc ggcgacaggt tgaccgacac
    16861 gaccgggaca tccagccccg cgccgcgcca ttgcgacaac tggcggcagg tctcgcgcag
    16921 ggcccagaac ccgagcgcat cgatctgccc cgtctcttcc gccatcggaa tgaagcggtc
    16981 gggagggatg gcgccgaccc gttcgttgtg ccagcgcgcc agcgcctcga agccgtagat
    17041 ctcaccgctg tccgaccgca cctggggctg gtaatgcagc gtcagctggt ttgcggcgat
    17101 ggcctgcttg agctccgagc tcagcagcag gcggtcttcg accatgacgt tcatggcccg
    17161 gcagaagaac tggtacgtgc ccccgcccac ggccttgacc cgcagcaggg ccgtcttgac
    17221 catgcccagc agatcgctgc ggctctccat cttgcccgcc atgcagcggc tgatgcccac
    17281 gctggccgac aggttcaggg tgtagccgcc gatctcgatc tgcccgttga cggcctccag
    17341 caggcgtccg gcgatgtgga cggcgccgtc cgcgtcgcag tccctcgccg ccagcaggaa
    17401 ctggtctcca ccgaaacggc ccaggaactg cccaggcctg agctggccct gcaggcggtc
    17461 ggccatttcc accagcacca agtcgcctgc gccatggccc agggcgctgt tgacgtcctt
    17521 caggcggtcc aggtcgatca tgaacagcgc cacgcccctg cccacgtcgc catccagcag
    17581 ggtgtcgata tgctggtcca tgtggctgcg attgggcagg ccggtcaacc cgtcgaactt
    17641 gatcaggcga tccatctgcg cgcgggtttc ttcccgctcg aaagccagca ggcacaggtg
    17701 cacgctggct tcggcgatgc gcgccagata ttccgacggc caaccgctgc gcttgaaata
    17761 gaaggcgaag gtgcccgcca cgcttccgtc gcgccgcttg atcggataag accagcaggc
    17821 ccggtagccg tacggcagca caatatgctt gtacccggcc cacaacgggt cggtctcgag
    17881 gtcggacacc atgaccggct cgccccggta ggccgccgtg ccgcagctgg ccaccccttc
    17941 gccgatctcc acgccgtcga atgcgagaca gtagtcttgc gggaaactcg gcgcggccca
    18001 gggcttgagc cgatggtcgg aaacgcggca tatcgacgct cgcacgccgg gcactatcga
    18061 ctcgatgcgg tggcacaggt atttgcccag gctgtcgaag gacatgttcc gggtcagcgc
    18121 ctcgaggcaa tcgcgctcca ggtcgcggat gcgccgttga cgatcctgct cttcagaatc
    18181 ctgtccgctt gcatcgtcgc gcgccagggc ggcgatcatg tgcaggcgct tgccttcgat
    18241 atcgacgagg gagcggatga acagcgcctt gccccgcctg ccatccttgc gctcgaccag
    18301 cagttcgcga gcctggccca ggccgtcacc cgagtcttcg aaattaccgc caaaataggg
    18361 cagccacgac atcgcgtcgc gcccaagcat ttcttcccgc gtatatcccc acagcctttc
    18421 ggcggcccgg ctgaaaaaca cgacggattg ggtatcgtcc agcaaaacga cggccagcgc
    18481 tgagcgatct agtgcagcaa atgaaatact ctcaacatcg gcaaacaccc catccatctt
    18541 ccgggcccct gcatggtttg cgatctgttc gtattgatcc ggtgatcgta tcgcacaccc
    18601 tgaaataaaa gcccaccatg ctgtttctgt ttgtaaatta tcaatggcct gcttatagga
    18661 cttattttca acgatgacgc acaagcccac gcacgacaac aacgccgcat tgcgccaccg
    18721 ggacgggcac catgcccgcg tgacttatga ggagctcttc ttcgacctgg tctacgtatt
    18781 cgcggtcacg cagctcagcc acgaactctt gcaccacctg aacccaatgg gcctgatgga
    18841 gaccgtgatc ctgtggttcg gggtctggct gggctggcag tacacctgct gggtcaccaa
    18901 ttggttcgat ccggagacgc cgcgcatccg gggcatgctg ttcgtgacga tgctgctcgc
    18961 gctgctgatg tcttccagca tccccgccgc cttcggcgac cgcgggctga tcttcgcgag
    19021 ctgctatgcc gcgatgcagg tggggcgcac ggccttcatc gtcagggaac tcgggcgcga
    19081 ccacccattg gccgccaact accggcgcat gcttgtctgg ctgtgcctct ccgccgtgtt
    19141 ctggatcgcg ggcggcctgc tggaccacga agcgcgcatc gcgctctgga tcgtggccat
    19201 cgcctgcgag tacttctcgc cgatgttcgg cttcgtgctg cccgggctgg gacgatcctc
    19261 gactcgcgac tggaccgtcg agggcggcca tttgactgaa cgctgccagc tcttcgtgat
    19321 cgtcgcgctg ggcgagaccc tgctggcctg cggggccgcg ctgaccgatg cgcaagcctg
    19381 gaatatggac atcctgtcag ccctgggggc cacgttcatc ggtaccttgt cgatgtggtg
    19441 gctgtacttc ggcacatcca gcaaggacgc caccgccgtc atcacctcgt ccaccaaccc
    19501 ggggcgcatc ggcgccaact tccactacat ccacgccatt ctcgtggcgg gaatcatcgg
    19561 cagcgccgta ggcaacgatc tggtattcgc gcatccgcat gccagcccgg aaaccgcgca
    19621 ggttctggcg ctggcaggcg ggccggcggt ctacctgctg ggcagcgcca tatacaagta
    19681 tgtggtttac ggccgcatcc cggtctccca tgtcgccggc gcgctggccc tggtggccct
    19741 gattccggcc tgctacggca gcaacctgct ggtcatgggt tggctcacga ccgcggtgct
    19801 gctcgccgtg agcttctggg aggcgcggat gcggcgccag cccatccttg gagagaacca
    19861 ggcgcatcat ccttaggtcc gcttgccggt ccgccccacc ccgctgccca tcttcccctc
    19921 aggcatcccc tgaacctccg cccgcggcaa aaacccgggc ggcccccctc cgcgtgcact
    19981 cgccaaccgc aaattccctg aatttgtggt tgacgacctt tttcacatta agtatcctaa
    20041 caacaattaa agtatactta ataagtaatt tcgactactt aatctctgtt ttgttggagc
    20101 acacaagtca tggaacgatt gcgctttggc atttgcgggt tgggctttgc cggttccgtt
    20161 ctgatggcgc caccgatgaa acagcatccc aacgcggaaa tcgtggccgc ctgcgacccg
    20221 aacgccgaca ttcgcgcggc tttcggcaag gactacggca ttgcgactta cgacagcctg
    20281 cgcgcgatgc tggacgacgc cgatctggac gcggtgtaca tcgcgtcccc tcatcagtac
    20341 cactgcgagc aggtcgtcct cgccgccgag cgcggcaagc acatcatcgt cgagaaaccg
    20401 ctcaccttga acgacgacga cgcccggcgc atggtcgatg ccgtagcgcg ccatcaagtc
    20461 cagatggtgg tcggcacctc gcgaagccac gaccccgtcg tcaaaaccat gcgggacatc
    20521 gtcggcagcg gcgagctggg ccgcctggcg atgatcaacg cctggaacta caccgactat
    20581 ctgtaccggc cgcgccgtcc ggaagagctc gatacgtccc aaggcggcgg cattctcttc
    20641 agccagctgc cgcaccagct cgatgtcatc aagaccatcg ccaacgcaac catcgtttcg
    20701 attcgggcgg gatcgggacg gctggatccg aagcggccga cggaaggcca ttgcgccgcg
    20761 ttcgtcacgc tgtccaatgg cgtgttcgcc acgcttgcct atagcggata cgaccatttc
    20821 gatagcgacg aactgcagtt ctggatcgcg gaaagcggcc gcgagaagcg gcccaatcac
    20881 ggcggcgccc gcaagcttct cgcggcgctc gcgcccgaag aagaagccgc cttcaaaaca
    20941 cagcgcctgg gttatggcgg ccccgtcgcc cgcaactacg cggcgaccga agccagccgc
    21001 aagcagccgc atttcggcat cttcgtcgca acgtgcgagc gcggcgatct tcgtccctgc
    21061 cccgaaggcg ttctggtcta tggcgatgag gggccgcgca cggtgcccgc ccacgtcggc
    21121 cggggagcgt atggccaggg cgacacgatc gacgaactct acgacgcggt gaccggagcc
    21181 gggccggctg tgcgcgacgc cacgtttgga cgaaacaccg tcctggcctg cctggccata
    21241 ctcgagtcgt ccaggacagg ccgcgaagtc gccgtgctct cttagcacgc ctgacccgca
    21301 atccaacacg accttatcca gcgttaaaga cctagggaaa acatcatgac tgatcatgac
    21361 gatccatatc gcatcctgac ggcagtcggt ccgggaacac cgatgggcaa tttgttccgg
    21421 cggttctggc tacccgccct gttgagcgaa gaactgccgg cggccgattg cccgcccgtc
    21481 cggatcagga tcctctgcga agatctcgtc gcgttccgcg acagcagcgg acgcgtctgg
    21541 atcatcgatg cctattgtcc gcacaggaga gcgccgctat ttttcggccg caacgaagag
    21601 aacggcctgc gctgcgtcta tcacggctgg aaattcgacg tcaacggcga atgtgtcgac
    21661 attccgaaca tcatcccgcc ggacaacttc gcggcgctca agcagagggc cagcatcaag
    21721 ggctatcccg cccaggaaag cggcggaatc gtctgggtct acatgggacc gaaagaactc
    21781 atgccggaat tgcccggcat ggaatgggtc ggcctgccag acgatcaggt caattgctcg
    21841 cgatggctgc accgcagcaa ctggctgcag gcggcggaag gcgaaatcga tacctcgcac
    21901 atcagctttt tgcacaaccg ggtcaacgtc ggacccaact cccccgactg gcagcgcctt
    21961 gcccaggatg gcgcgccgca gatcacggtg agggaaaccg actacgggtt cgtctatgga
    22021 tcgcgtcgaa gcttcgaagg gcagtatttc tggcgtgtca cgcaatggat gctgcccatg
    22081 tggagcgcgg ttccgcggtc catcggcgac cccatcctgg ccggccgcgc gtgggttccg
    22141 gtcgatgact acacaacgac aggcttcggc tacctctatg ccgccgacag gccgctctcc
    22201 accaaggaac gggaagaagt cgcgtccggc gaacggtttc cgccgcgcgc caggaaggga
    22261 accttcaggc tgccgcacgg ctacgtcatc gacaccttcc tgccggacgc gaatatcggc
    22321 aacgacttcc tgatcgaccg tcagatacag gcgacgacca atttcaccgg catattcggc
    22381 gtcaacgaac aggaccgggc cctgcaggag tcgatgctga gcattccggg gcatgtcggc
    22441 attgttgacc gctcgaaaga gcgtctcgtc aaatcggatc ttccgatcat gacggcgcgg
    22501 cgcatgctta cgaaactggc caaggatctg atccagggca tcgagcccaa ggccgcttcg
    22561 agcgcggacc agtatggcgt gcgaggcatc gccatcctgt cgcccattgc cgacttcgat
    22621 gagttcatgc gggtccacgc gaactcgatg gaagccgccg aggcatagga tggcgatcaa
    22681 cggagactct cattcgcata tcgaggccag gctccactcg atcacgcagg aagccgaaga
    22741 catcttgctc ttcgactttc gcccaggcct tggacagcat ttccccggcg tcgaagcggg
    22801 cgcgcacatc gatctgcacc tcgccaacgg actcagccga agctattcgg tcatcaactc
    22861 gcaggatgac cgcgaccgct atctgatcgc ggtaaaaaaa gaggccgccg gccgcggcgg
    22921 atcgcgattc atccatgatg agttgcgcgt cggccaacgc atcgccatcg gcgggccgcg
    22981 caacaatttt cgcctgcggg aggcgtctgc ccattccgtc ttcatcgcgg gcggcatcgg
    23041 catcacgccg ctctacagca tgattcaacg gctggaacgg ctcgaacggt cgtgggagct
    23101 gcactatgcc gcggcccatc gcgagcaggc cgcgttcgtc gagccgctgg cgacgctgca
    23161 gcccaagacg catttctact tcaagtcgga ccaggccggt caaggcgcgc tggatctccg
    23221 gcagatcatc cgggacagcc gccctgatgc cgatttctat tgctgcggtc cgactcgcat
    23281 gctgcgggat ttcgaagcgg caacggcatc gattccgccg gatcgtgtgc acgcggaata
    23341 cttcaccgcg ctcgaggcgc ctgcggccga aggaggattc gaggtcgtgc tgcaacgctc
    23401 gggccaacgc ctcgccatac aagcgggcga gacgatcctc gacgcgctga tcaagctcgg
    23461 gaccgatgtg ccctattcat gcatggaggg cgtatgcggc agctgccagg tccgggtcat
    23521 cgaaggcacc cccgatcatc gcgacctcat cctgagcaag caagaacgcg acatgaacga
    23581 caggatgctg atctgctgct cgggctcgaa gtccgcgaaa ctcgttcttg acctgtagcg
    23641 agcccaggct tcatcagggg tcccctgcgg tcacgcggcg ttcgcggccg cctgctcgcg
    23701 cgcgtactgc gtcggcggcc tgcccacgtg ccgcgtgaag gccacgctga acgtgctagc
    23761 ggagcggtag ccgacacgct cggcgatctc ggcgacacgg ccagcgttgc ggcgcagcag
    23821 gtccttcgcg agcgccatgc gccaggcgag cagatattcc atcggcgtga ccccgaccgc
    23881 gcggttgaag cgctcgaaaa aggccgagcg agagagcgca gcctccttgg cgagctcgac
    23941 aaccgtccac gcgtgcgtcg ggcgcccgtg catcccgcgc agcacggcgg cgagacggca
    24001 gtcggagagc ccgcgcgcca ggcccggcga cgcattcgtt cccgccgtgg accgcaacgc
    24061 ctcgatgagc agcacttcca gcagtcgcga gagcacgacc tcgcgcgcgg gccgctgctc
    24121 gcgtgattcc tctcgcacaa gctgcacgag cgtggccagc cgccgttcgc cgcggacatg
    24181 cacgaactgc ggcagcagcg agaccagcag ggacgcatcc ggcgaaccga agctgcagtg
    24241 gcccgccatc attcgggcgt cgatcggact gtccgggccg ccgatcctga actcgccgtt
    24301 gcccagcgca acgggcgccg gcgtctcgac ccctggcggt ggcggttcga ggctggacat
    24361 ctcgacgcca taggccgcgg gaatcagcac gaaatcaccc gacagaagct cgatgggctc
    24421 gtgtccgtcg atcgtgacgc ggcatccgcc ctcgagcatc acgcaataga acggctggcc
    24481 ggcatccgag cggctgatgc gccaggggct ggcgccgaag acgagcttgg agaaccgggc
    24541 gcccggctgc agcagcgtca cgacttcggc caagggatcg accataaccg gactcctgca
    24601 aatgaaatgc ggactccgga ttgtagtgcg ttcggcccca gccacctatt gtgtagccat
    24661 cgccaatcac tattgaagga gtttccatga agacagttct gatcaccggc tgttcgtccg
    24721 gctttggcct cgagatcgcc cgctacttcc tggcccgcga ctggcgcgtc gtcgccacga
    24781 tgcgcacgcc gcgcgacgac gtgctgccgc attccgatcg cctgcgcgtg ctcgccctcg
    24841 acgttaccga tccggagagc atccgcaagg ccgtggaggc gtcggggacg atcgacgtgc
    24901 tcgtcaacaa cgcggggata ggcgcggccg ccccggccga gctcacgccc atggcgacgg
    24961 tgcgcgagat cttcgaggcc aacaccttcg gcacgatggc aatgacgcag gcggtgatac
    25021 cgcaatttcg ccagcgccag accggcgtcg tcgtgaacgt cacctcgagc gtgacgctga
    25081 gggcattgcc tctgcttgcc gcgtacagcg cgagcaaggc ggcggtaaac gcatacaccg
    25141 aatcgatggc gctggagctc gaacccttcg gcatacgcgc gcgcctggtg ctgccgggcc
    25201 gctcgcccga gacacgcttc ggcgagaatg cgcgcaccca catgcatggc ttcgaccacc
    25261 cagcctatgc cggcctggtg caaaaggtct tcgcggcgct gacggacacg tcgtcgccag
    25321 tcacctatgc gcaagacgtc gccgaagccg tgtggcgcgc ggcgaccgac ccgtcgtctc
    25381 cgatgcgcat cccggccggc gccgacgccc aagcgtgggc cgccgaggcg cggcggctgg
    25441 ccggggataa cactcgcgta tttgactgat tgttccagta ttgctataaa cgcctccatg
    25501 ccgagaccga aggaattcga cagggacgaa gcgctcgacg cgtcaatcgg cgtgtttcga
    25561 gagcatggct tcgcgggtac ttcggccggg atgttgaccg atgcgatgaa gatcggtcgg
    25621 cagagtctct acgatacgtt cggtgacaag tggcggctct actgcgcagc ggtgcaacgg
    25681 tatgccgttg ccgaagcgca ggcgcatttc gcagcgctga aaaccggtcc caaggccatg
    25741 gacggcattt gccggatgat ggaacgcgtt gtggccgagg cgcgaatgcc ctgccttggt
    25801 gtgagttcga tcagcgaatt cggcgatagc cgcaaggacc ttgcgcagtt gcgtaacgcc
    25861 gcgggccgcg ctctgcgcgc ggctatcgct gacaaagtcc ggcaggcgca agccgagggc
    25921 gatgcagccg tgaagcttga tccggggcac gcagccggtt tcctcatcgc caatgtggcg
    25981 gcgatcagac tcgccgcgcg cgcaggagcg ggagatgctg aactccgggc gctcggacgg
    26041 cttgccatca ccgccctgag gtagtttttt ttgccatatt ctggaatgaa cattcaaaat
    26101 aggagtcgaa taatgaaagc tatcgtcatg accggcacag gcggcacgga aacgctgcgg
    26161 catgtcgagc gccccgaccc ggcgccaggc gcgggccagg cgctggtcga ggtggccgcc
    26221 gccggcgtca atttcatgga tatcggcgtc aggcagggca tggcgtggcg cgacgtaccc
    26281 aatccgaaaa tcctgggcgt cgagggcgcc ggccggattg tggcggtggg cgaaggggtc
    26341 gccggcttca cgcccaagca gcgggtggcc tgggtgtacg cgccgggcag ctatgccgag
    26401 aaagtcgtca tcccggccac cgcgctcgtg cccatacccg atgaggtgga cgaccggacc
    26461 gctgcctcac tgatgatgca gggacttacc gccagccact tcggaacgga tttccacccc
    26521 gtgcagcccg gcgaattcgc gctgatccat gcggcagccg gcggtgtggg cctgcttctg
    26581 acccagatca tcaagatgcg gggcggccat gtcattggac gcgtctcttc ggccgccaag
    26641 gtggcggtgg cgaaggtagc cggcgccgac catgtgatcg tggatgccga gggccggttt
    26701 gccgaggaag ccatacggct ggcgggcggc gaaggcgtgc acgtcgttta cgacggctcc
    26761 ggccccaaga cctttcaagg ttcgctcgac gcgctgcggc ggtcggggac cttctgctgg
    26821 tacggtcccg tcctgggcgg gcccgggccc atcgacatca tgagcctgcc caggagcatc
    26881 aagatcggct acgcgacctt catggaccac atccacactc cggaactatt gcgtgcccgc
    26941 tcggcccggc tgttcgactg ggttatcgag ggcaagctca agatccggat aggcggcgta
    27001 tatgccctgg cggatgcggc gcgggcccat gccgacatgg aaagccgcgc gaccaccggc
    27061 aagctgttgc tgatcccatg atcaggctgg cgtgacggcc accggttggg ctgggccgct
    27121 atccgaggtt acactttcgc gttgaatagc ggtctacaaa acccctcagc gggtccaagc
    27181 atgcgccgaa ccattttatt tttgcaaggt ccaccctctc ttttttggcg tcaactgagc
    27241 gaacacttcg aggcgtccgg ggcaaaaaca catcgcatca atctttccct cggtgactgg
    27301 gcctactggc gcaaacgcgg cgccgtcaat taccgcggcc gcttcagccg ctgggccgac
    27361 tttctccgcg attatctggt gcgccaccag gtcaccgata ttctttacta cgcggatcgg
    27421 ctgccctacc acgcggtggc gcgcgaagtc gcgcgctcgc tcggcatccg cacctacgcc
    27481 gtcgagttcg gctacctgcg cccggactgg ctgacgctgg agcgcggtgg catgggcacg
    27541 ctctcccatt ttcccgacga cccggccgcg atccgtgcga tcgcccgccg cgccggtccg
    27601 ttcaggcctg agaccgcgca ctactcgcac acgtttgggc aggaggcctt caacgaggtt
    27661 ttctacaacc tgcttacctc gctggtgccc tacttctttc cgctctacag gacggacaag
    27721 tactacctgc ccctgcgcga ctatctgagc tggctgcctc gcatggtctc ccagcgcctg
    27781 cgcgagcccg cccgtgtcgc ggccgccgac gccatgcgcc actccaacaa gcgctactgg
    27841 atgctggccc tgcaaatgca ggccgactac cagctgcgcg tcaattcagc ctatgaccat
    27901 caaggccgca tgatcgagga ggtcattgcc tcattcgcgc agcacgcgcc gcgagacgga
    27961 gccttgatcg tcaagctgca cccgctggac aacggcatcg agcgctggga aaagttcgtg
    28021 acccgcaccg ccgcgcgcca tggcgtggcc gctcgcgtag tcgctgtaac cgaatgcgac
    28081 ctcaagcgca ccattaaacg cagccagggc gtcatcacgg tgaactccac cgtgggcctg
    28141 catgcgctgc gcgccaactg ccccgtcaaa gtgctgggca tggccgtcta cgacatcgca
    28201 gggctcacgc accaggagcc gctggacacg ttctggagca agccccagcg agtcaacaag
    28261 gccctgctga aggacttcgt gtcggccatt gccgccacca ttcaagtgca cgggtcgttc
    28321 tacaaccccg ccggccgcaa ggcggcttgc gcagaaatcg tgcgccgcgt gctgaacgat
    28381 caggtcaacg cactcggtgc gttcatcgac ccgccgccga gactcgggcg cgcactgcag
    28441 gccgaggttc ccatcttcag ccccctgcgg gatagcggcg tggaaccggt gctgacagcc
    28501 ggcaccgcta cgcctcacga aacagccggg caacccagtg ccgccaactc ggcacccgcg
    28561 gtctagcccg ccactcgatc aattccctca gcgcgcgctc cggagtcgtg aagcggcccg
    28621 tatcgcggct cacgtacgtc ggatacagaa tcagcgctgc cgcgacgagc tcgtccagcg
    28681 tcaacgcacg ggcacgcctg cggcgcagcg catcggtcag ctcaacgtcc cgcgtcaagc
    28741 cccagcccgc gtagaacggc tggccgtagg tcaccacttt cacgttgcga agcaaggctt
    28801 cgaagccggc cagcgaagtc agtacgtgca cttcgtccac gctgccgagc agttgatgga
    28861 atgacgccgc gccgatgatc tcgtcgcacc atcgcgcggc gctgttttca tcgcggccca
    28921 ctttgcgcag tccggccatg acatccggat gcggtttgta aagaatgtac gcatcgggat
    28981 tcgcagcgcg tactgcctgc aagagcgcca tgttgccgcg gatggccgag gcgccataac
    29041 ggatcgaggc gtcggtttcc acttgccccg gcaccaggat cacgcacttg tccggcggcc
    29101 ggacccagtg cgccgcgtcc gcgaggttgt acttggtaat gccggcgtcg caaatcgcct
    29161 cgcgcaggct tgccgcgcgc gcgaccaacg ccggggtgaa cgccgtctgc gccagcagtt
    29221 gctccaggcg cgacgcaccg gtggcgtcgt aataaatgcc cacgtcgtcc tgcacccagg
    29281 acagtggccg ggtcaggtcc gcacccaggc cgacggagcg gatgaaccca tcctcgacac
    29341 gaatgacctg gcccgttccg gaagggttcg aggccagccg gccatcgagc ttacgccccc
    29401 aggtcgcgac agcgccgcgg gtcgagcgcg gcacgcccca gcgaaagcgc accgcgctgc
    29461 cgccgaagta ctggcgcacg tagcccttct tccatccgga aaatcccagc gcgtcgatct
    29521 tcaggtcgaa acgctgccgc atgcgacgct gcagcgcaag ccacgccagc agggtttcga
    29581 cctcgcagcg cttgccggtt tccgggtcga cgtagcggca gtagtcgatg agcgccgcat
    29641 gaaccagttg ctcgagcgtc gcggcgccgc gacgcgcggg cgccggcagc tcgtcttgcg
    29701 tcaagcccca gcccgcatag aacggcatgc cgaacacccg caccggcagc ccccacagca
    29761 gcgcctcgaa gccgagctgc gaggtcaccg tgtacgccgc gcgcaccgcc gacagcacgc
    29821 tggacggatg cacgtcgtcg tccagcacct gcacgcgcgg catcgcgcgc accgcgtcca
    29881 gatcgaagtg gccctgcttg cggccggaca cgacgtccgg atgcaccttg agcaggatcg
    29941 tgctgtcggg gtgttcgcgc agcgcggcgg ccagcatgcg ctcgaagctg gcagcatccg
    30001 cgagaccgca ggcaaccgag gcgtcgccgc gcgtctgatc gacgaccagc acgcagtcgc
    30061 cgggcagcgc cctcgcgaga tcgcggctgt ggttgtactt ggacacccgc ccgtcgcacc
    30121 acgccgacct cagcgcccgg gcccgcgcgg attgatcgcc gtcgagcggc tgcccgatca
    30181 ggacctccag cctcgatggc cggctggcgt cgagatacag cccgaggtcg tcgaaaatga
    30241 tggcaagcgg cggatcgtcc ttgccgagcc ccaccgagcg cagaaagccg tcttcgatgt
    30301 gataaacggc gaggccgcgc cgctgcgcgt attgctgcgc gcgaatcgcg ctgggccgcc
    30361 ggccccacgc caatacaccg gcattgccgg ccggccggcc aaccggccgg tagcgcagtt
    30421 ggaaatcgtc gagcagcgca gccagcgtcg ggatgccacg cacccctggc gccagcgtat
    30481 acgcgagcgt cggcggaaca gcgcgcgcac gttgacgaga ctgctcgttg ccgacctcat
    30541 cgccatgcgt cgggaaatca gttgaaaaca tcatccgccc tgaaactatc ttgaatcact
    30601 cccacaccat tattaacagt agcaagccat cgctcaagct cccctcgggc acggatatcg
    30661 ccgccagctg cggtcgccat tcccatgcat tcggggcgat ggcattgcac ggcgccaagg
    30721 gagtaccttt taagcacgtc ggacttacca ggaga
//
LOCUS       DASWPO010000060        28325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2781193, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000060 DASWPO010000000
VERSION     DASWPO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 28325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28325
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781193"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VGC69_03640"
     CDS             <1..1339
                     /locus_tag="VGC69_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0294411.1"
                     /translation="PDSDDLLSGVVLSASPTEVKVARSSTETITINDKKALSVIARAL
                     SSKAKSEVRITRGSVVYVHKNGGTWEIINMPTLQAAMVSLAPQDGSIRAMIGGFDFQR
                     GSFNRAVQAWRQPGSNIKPFIYASALERGLSTTTEISDQPFFMSAAQTGSKDWAPRND
                     GDEYEPELTLRQGLYKSKNMVSIRILQAITPQYAQNYLTRFGFEADRWAPVLPMALGT
                     GSATPLQVAGAYTVFANGGYRVPPFLIDHVTDRTGKVLMQAQPIRAGDETARVIDART
                     AWLMDDVLRGVATYGTGARAHAVLKRNDVAGKTGTTNGAADIWFSGFTPSLLTTVWMG
                     FDQPRPLGTHEFGSGVALSTWLGYMQPMLANVPQTPPPPMPQGLIQANGDYYFSEFPP
                     GQAVASLGLSGSDGNGVPVTNDSDVLGGLLDKVWSSLKEGPAGNRIETQKATP"
     gene            complement(1377..2207)
                     /locus_tag="VGC69_03645"
     CDS             complement(1377..2207)
                     /locus_tag="VGC69_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0294412.1"
                     /translation="MAQVPTIALNDGNRIPQFGLGVWQVPNDEANEAVQAALAAGYRA
                     VDTAAIYGNEEGVGSGLRASRLARKDLFITTKLWNSRHGYDEAHKAMDESLTKLGLAY
                     VDLYLIHWPVAGSERFMDAWRAMIEMRADGRARSIGVSNFTQANLRQLIEGTGVVPAV
                     NQIELHPAFTQVDLRVFHAKHGIVTESWSPLGQGKMMDHPVLATIAGQHGKTPAQVMI
                     RWHLQHGLVVIPKSVTPARIRENIDVFDFTLSAEDMAAIDDIPEAGRLGPDPERFAMA
                     "
     gene            complement(2348..2557)
                     /locus_tag="VGC69_03650"
     CDS             complement(2348..2557)
                     /locus_tag="VGC69_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294413.1"
                     /translation="MNHPGIRQGELAYWLGVIAEPGGEAEPVPAHIAEALQILRCIEP
                     QGSGFRITNKGQLALRMADPAALWT"
     gene            complement(2554..2907)
                     /locus_tag="VGC69_03655"
     CDS             complement(2554..2907)
                     /locus_tag="VGC69_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY0294414.1"
                     /translation="MPLSLEKLLPIGLLALSNIFMTIAWYGHLKFPSTSLVKVVLVSW
                     CIALAEYSLAVPANRIGHRVYSTAELKTMQEVITLVVFSIFSVFYLKESFTINHVVGF
                     GLIFAGAFFIFKGPL"
     gene            complement(2996..3724)
                     /locus_tag="VGC69_03660"
     CDS             complement(2996..3724)
                     /locus_tag="VGC69_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEY0294415.1"
                     /translation="MFGSSRKTVFKPSTFEPGRRDRRVPRWLVLLCVGIVLGAGGLLF
                     FQHNYGPQQLTVEASQKLLSDLNSVTLERQSLQAQLTEAQSQREQSQSTQEKATGDLA
                     QANERIATLTQTLQILQDAVPADPRGGNIGVRWGEFVSRDGGVGYRALIMREQADKLS
                     AFQGQVVVELAGTRNGRADTIVSQPASFTLERFAPLQGTVPLPDGFVPRQATLRVSDA
                     QGRAQAMRVYFVRGVGRQQGASQP"
     gene            complement(4003..5004)
                     /locus_tag="VGC69_03665"
     CDS             complement(4003..5004)
                     /locus_tag="VGC69_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0294416.1"
                     /translation="MKRWTHRPEGSTWGDWGEDDQLGRLNLLTPARVLRASREILTGE
                     RFCLSLPLDYPGGSVLAPHRKPPQLAPTMRSGRPFANYAFGQEGPFCDCGNDDIVTLC
                     TQYSTQWDSLAHIGCRFDAHGTGQERMTYYNGYAETDIVAPGEPGKATMPLGIQTMAA
                     AAIQGRGVMVDLHAHLGAQRRRVGYDTLMEIFEADGLAVEEGDILCLHTGFAERLLSM
                     EGKPDLSVLNNACAVLDGTDERLLQWVGASGVAAIVADNYAVEGFIPVIGLTPRPMVP
                     LHVHCLFKLGVPLGELWHLTPLAEHLRHHKRFRFFLSAPPLRLPGAVGSPVTPIATV"
     gene            complement(5013..6902)
                     /locus_tag="VGC69_03670"
     CDS             complement(5013..6902)
                     /locus_tag="VGC69_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0294417.1"
                     /translation="MKAANPLPASTSRSAGSHGLRIVRRIDRGLVTIVNIIASILVVV
                     ETALLFAGIVARYVFQMPLIWSDELAEYLFLWLAMLGAVLAYRADEHMKMTAVVDRLP
                     QVWRDRLDAFVRLACLVFLCALALPALSLADKQAMIHSPALGLPLSWRYRAVVAGVVL
                     MGLFEILRLFSEPVAARRVAGSAIVLALLGVGVWYARDAFTGLGQWNLLIFFVGVVVI
                     ALVLGTPIGFAFGLASIGYVTFATHVPSLVWVMRMSAGMTDLILLAVPLFIFLGLLLE
                     LSGMAKAIIDFLASLLGHVRGGLSYVLIGAIYLVSGISGAKAADMAAVVPILFPEMRK
                     LGTPPGELVALLSATGAQTETVPPSLVLITIGSVTGVSVAALFAGGLLPSLLLGLMLC
                     VYVGYKARRQTTGVSPRAGFAHVRRIFVRALPAIALPFVIRYAVVEGIATATEVSTIG
                     IAYCLIVGAILYRHLEWRKLGPSLAKTATLSGAVLFIMGTATAMGWAITQSGFSQQLA
                     QMMSAIPGGSATFLAASILLFVLLGSVLEGIPAVVLFAPLLFPIAKELGVNEIHYAMV
                     IILSMGLGLFSPPMGIGYYTACAISRIDPNAGLKAIWGYMAVLAVGVLIVACVPWLST
                     AMIVK"
     gene            complement(6899..7909)
                     /locus_tag="VGC69_03675"
     CDS             complement(6899..7909)
                     /locus_tag="VGC69_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0294418.1"
                     /translation="MVTRRKILGMGLAAGAIPWVHTAKASTAQYTFKYAGNVNDRHPL
                     TIGVANAAKMIAERSSGQIRIDLFPNSQLGSDQDMLTQLRVGAIDFFTMSGILLADLI
                     PTAGISGVGFAFTSIDQVWPIMDGDLGNHIRQSIEKAELQALPYIFNGGFRDITTSNR
                     PINTPDDLKNFKIRVPVSPLWTSLFSALGTLPASIGWAEVYTALQTHVVDGQETPLIS
                     VEASKLYEVQKYCSLTEHMWDGFWMLVNPGSLASLPPPLRKIVNDSFAEATLQQRKDS
                     TVANDTLQGILEKKGMTFNRPDPEAFQATLRAAGFYKQWQGKFGSQTWTLLEKGLDKR
                     LA"
     gene            complement(7966..8796)
                     /locus_tag="VGC69_03680"
     CDS             complement(7966..8796)
                     /locus_tag="VGC69_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0294419.1"
                     /translation="MLNPQSDIREVPAGNRLEGKVALVFGAGSSAPGWSNGKAAAVVY
                     ARQGAQVVCVDIGLPAAQETADLISGVGGQATAHAADVTIEADIARVVRAVREAHGRI
                     DILHNNVGMTSMGDIESLPMERWQKAVEINLFSVVHACRQVLPVMRNQGGGAIVNISS
                     LASIQINEYPYPAYQSTKAAVNHLTRSLAVNYAPHNIRVNAVLPGVMYTPLIDTQIAG
                     NYANRDEMLAKRHAASPMGHMGDAWDVAYASLFLASDEARYITGVIMPVDGGKACAAR
                     "
     gene            complement(8789..10516)
                     /locus_tag="VGC69_03685"
     CDS             complement(8789..10516)
                     /locus_tag="VGC69_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0294420.1"
                     /translation="MKLEMTGGEAIARIFQAANIGPIFGMGGFQTLPFYDATRRLNMP
                     HHLINDERAAVFIADAYSKASNGRVGAADATLGPGATNLVTGLVEALNAGTPMVVIVG
                     DTHRDHSWKNMTQESDQIAILKPACKAVIRIEKISRIPELMKRAFAVATSGRPGPVVV
                     DVPEDIAHDTCEFDEAEFSIAAHYQAAPALRVRPDVQSLAAAAALLRGARKPILLAGG
                     GVHLSNAHEALTAFSRAFNIPVAHTLTGKGAIACTDPLSAGLFGRYDRIANGLIDEAD
                     VVIVAGCKLGEIATKRYTVPGPGKCLIHIDNVAEEFGRTLEPTVALWGDVRATLEELD
                     TLIRPQQAEMAARLGSWAAQVPARMAKWREEVGPRLHSEEVPINMARLITELNHVLPE
                     DGILIADGGFAAHWGGLLFDTKAAGRRFIPDRGFASIGYGLPGAMGAALAQPSVPVFA
                     LTGDGGFNMMLGELETARRLGLNFSVLVVNNAASGYIKALQHLLYGPEGYHASDLAET
                     NYAKIAEAMGVNGLRLEKPEDIRATLQKAMTLKGPTVIDVVVTRDASKMLPGVDNRAV
                     KMKKGDRVA"
     gene            complement(10562..11401)
                     /locus_tag="VGC69_03690"
     CDS             complement(10562..11401)
                     /locus_tag="VGC69_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0294421.1"
                     /translation="MSMEQKNLEDDLAGTKLADSLAPSVRILERATALVDALSEAGDR
                     GLNLAELVERTQLSKTTTHRLAHALTAVGWLYSDADSKTFHLGVRLAGRGAHAWQYLA
                     ALLARPTLMALAEETGDTVFLSVREGSGAVCVARHEGGFPIRTLTLNLYDRRPLGVGA
                     GALALLAWAPPAWRERYLEINEQWIASYPRISIPLVRELMANARTNGYAINDGGIIDS
                     MIGIAVPILNEQQEVIAALSIAAISSRLDEKRRLQLVPDMQARAAVLAGQFSARFASL
                     PIN"
     gene            11479..12324
                     /locus_tag="VGC69_03695"
     CDS             11479..12324
                     /locus_tag="VGC69_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294422.1"
                     /translation="MPDLLPIALADTATTLHATEAGKVVVCGSHGALYPAALLLAAKV
                     RGSILYDAGGQGQTGLAVQEGLDLLERHGLPGALCSVHASRIGDAADAWTRGMISSCN
                     GLAARLGVQPGMTVRDAAERLTHASWDAAAVPTVAPPRETRCVIGVGELHVVLVDSAS
                     LVEPQDAGKIVITGSHGALVGNDPAKALAVDAAAAVFHDAGMGIGQAGIQRLGALARR
                     GIAAVTVAADSAPIGCAKAIWDTGIISAANETARQTGASNGQSLQAWVWQLGDLESRG
                     QAGRR"
     gene            complement(12400..12476)
                     /locus_tag="VGC69_03700"
     tRNA            complement(12400..12476)
                     /locus_tag="VGC69_03700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12440..12442),aa:Pro,seq:ggg)
     gene            complement(12513..13526)
                     /locus_tag="VGC69_03705"
     CDS             complement(12513..13526)
                     /locus_tag="VGC69_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY0294423.1"
                     /translation="MSASAIWLSENDVSGLVSLKDAIGALEDGLRALGSGDGFNIDKA
                     LGSFGSASSMHSLGSALPGVGFCGYKNWINTPQGAKALYVLFDAVNGRLLALMEANVL
                     GQLRTSAMTGLGTKWLTRPGVDDMALIGSGRQALAQVAAVHLVRPLARLRVWSPTPEK
                     RKAFCETLRAQFDFDVVEAATLREATAGAPIVTTVTRARTPFLEAKALDKGTHLNAVG
                     AILPASAEFHQDVFNRVGFIAVDDVSATRKASREFIDYFDQGAGAGDWARVHPLSEVI
                     AGGKGIAGDTDLTLFKSMGMGISDLSVAMLAYQRAREAGLGLEIPLTAGAAIRLVGDA
                     PGI"
     gene            complement(13542..14399)
                     /locus_tag="VGC69_03710"
     CDS             complement(13542..14399)
                     /locus_tag="VGC69_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0294424.1"
                     /translation="MKLVSYLDPGKDPARPSYGAVVGDAVVDLASPSAPTLRAALAGE
                     GIAGLAGRLAENRGGSTALTDVVLLPPVIDPAKILCVGLNYHLHAQEAGMQVPAKPSI
                     FVRFASSLTGQGQPVLRPRESDLFDYEAELAVVIGRQGRRISEQDAMRHVAGYACLAE
                     NSVRDWQRHSAQATPGKNFDASGAFGPWLVTPDEAGPPENMDIIGRLNGEVVQHDSVA
                     NMIFSVAELIAYLSTFTELLPGDVISTGTPAGVGLGRKPPRYMRAGDVFEVEISRLGV
                     LRNRVQDDI"
     gene            complement(14469..15275)
                     /gene="hpaI"
                     /locus_tag="VGC69_03715"
     CDS             complement(14469..15275)
                     /gene="hpaI"
                     /locus_tag="VGC69_03715"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HEY0294425.1"
                     /translation="MVKENLFKRALASGQSQIGLWLGLAQPYTAEMLGGAGFDWLLID
                     GEHAPNTLTTTLAQLQALASSPSHPVVRCAWNDTVEIKRLLDIGAQTLLVPMVQDAAQ
                     ARAAVEATRYPPAGVRGVGSALARASRWNRDGDYLQWANDQICVLVQVETQAALSQLE
                     PICGVAGVDGVFIGPSDLAADMGHLGNPGHPDVQRAVEDAIARIGRAGKAAGILIGDE
                     ALARRYIELGTTFTAVGVDTTILARGAEALAGRFKAAGTTPQRTGGPSVY"
     gene            complement(15280..16083)
                     /gene="hpaH"
                     /locus_tag="VGC69_03720"
     CDS             complement(15280..16083)
                     /gene="hpaH"
                     /locus_tag="VGC69_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEY0294426.1"
                     /translation="MLDDALIKSLAREFFDAEKSRQQIRQVSLRHPGITIDDAYAIQR
                     EWVAMKIAGGNALKGHKIGLTSRAMQTAVGIDEPDYGALLEDMFFYDGCTIPTDRFIA
                     PRIEVELAFIIEKRLAGPGVTLFDVLDSVRYVVPALEIIDSRTQMVDPETQRTRKVFD
                     TIADNAADAGVVLGGRPVRIGEIDLRWVGAILMQNAVIEETGIAAGVLNHPANGVVWL
                     ANKLAAFGVALEKDQVILGGSFTRPVFAKQGDSFHVDYGSLGSISCHFG"
     gene            complement(16129..16539)
                     /locus_tag="VGC69_03725"
     CDS             complement(16129..16539)
                     /locus_tag="VGC69_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0294427.1"
                     /translation="MTEVDLNAVSAAVYRFFLGLDQRDNQGVAALMAREGVWHRQGAE
                     LVGPQAVLQALEKRDPKRRTAHTVANLWVEAATPQTARVRYYLIAYETTVDAQGRESE
                     PKMLGVRESTDDLILEDGAWRVRRKHSRRFLPAE"
     gene            complement(16569..17675)
                     /locus_tag="VGC69_03730"
     CDS             complement(16569..17675)
                     /locus_tag="VGC69_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294428.1"
                     /translation="MRGLVFADVSGEPSKKPQPTTPFLVTREDIDAEIARLADIARQA
                     NGRRASKIVHPAASDGMLSLTPSTDVAIQVLKPGEETLDYRSNANEIEICIRGSGRVI
                     AADATHEVGRFDVWNIPAMRVHRYKNTGSDLWVRLTYSNAPLLEKLAVFFSEEGDEIK
                     SQETRQDGLSPVTKKKYSREEAPDMPIGSEGARLRGYEFLTDIEVVENKTLLWRWQDV
                     RPYLPMRVGDGHRPIWLLYNPATEKRAGTNNSFFATWTGAVPGMPPFAGKRGHRHTSA
                     SINYHASGHGTSVVDGVTVEWKAGDLLFSAPGWSEHAHYFGSDGWEVLTIQEHPMHIA
                     LGSLLWQEKIDGPIYALGAERGQAGYQGPREVGA"
     gene            17801..18709
                     /locus_tag="VGC69_03735"
     CDS             17801..18709
                     /locus_tag="VGC69_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294429.1"
                     /translation="MDLHRLDLNLLKIFDSVYRLRNLTEVAHEVSLSQPAISHALGRL
                     RTALDDRLFIRTATGLEPTARADSLAIPVRNALAMLEDSLLSSEEFKPEHCAREFRLL
                     LSDVGNVLFIPPLLSHLHRHAPQVRLKIIQAPRSDYERLLRDREADIAIGHLNLSGQN
                     IRKAPLFRDRCVILSRAASTGKSRRPMSREDFEQRPHVIVSPPEDPVEEMFSSLHIVR
                     RISLHIPSYFALPGIVSTSELLATVPSRIAMNLSKNFSFDMHELPFASPVFDVKMHWH
                     LRQDADVAQTWLRNIFHALFAERAAE"
     gene            complement(18748..20049)
                     /locus_tag="VGC69_03740"
     CDS             complement(18748..20049)
                     /locus_tag="VGC69_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294430.1"
                     /translation="MSGVYSSATLVQEHPSTQEKSKASGSVALVIAAALGLSSGFTAL
                     FVGTTGVFLLPIAQTLGVGRGAAASCVISASLGLAISSPIAGRLVDRYGFRKVISVSI
                     LLFVLGLLSLSVGPYNVTALSIKTFLIGLLGVATSPVGYLPILARSFERRLGLALGIA
                     SVGAGIGAAVAPLVAGGLIQRHGWQFTYMVLAATAAVLGALATYLIYRFGGSAAEPAV
                     KPSARDEAVLPGMTARESLAHPRFWVMSISIALVAAVGLGGMVHIPAMLKDNGLSVEL
                     AAAGSSFAAIGITCGRLLAGVLLDLITARVLSAILFLLGALGVLILATVSHSTPYPLL
                     GLGAALTGMLIGAEGDLMPFLVKRYFGLRSFGMVYGLMISIFAIGTLLGPILFGAAFD
                     RLGNYHLAMEVAIVACVVGAIGILTVGSYRYASDQRAERPS"
     gene            complement(20202..21104)
                     /locus_tag="VGC69_03745"
     CDS             complement(20202..21104)
                     /locus_tag="VGC69_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294431.1"
                     /translation="MDLRRVDLNLLKIFESIYRLHNLSQVAEEVALTQPAISHALARL
                     RDTVGDRLFVRTAAGLEPTSRADELIGPIKAALAMIEESLNASSEFDPRTCRREFQLL
                     LSDVGELIFLPRLLQYLRKEAPGVKVSVRQASRTRYEAMLRDKDADLAVGNLPMMGKG
                     LSRSHLFMDKWVVLRAKDAAHKPRLTVREFERCAHVLVDPPGTVDHPLTELLKQKNIA
                     RNVILTIPHFFALPSVILSSDLLSIVPYSVVVNLRSAAGMQVQALPFKAPALDICMYW
                     HARRDADLAHAWFRGVFARLFSKK"
     gene            21285..22397
                     /locus_tag="VGC69_03750"
     CDS             21285..22397
                     /locus_tag="VGC69_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294432.1"
                     /translation="MTEQTFRFVEKRQIFSDQNAVWPPMRVPAEFIDREIERLADATL
                     AEGDLRAASIVHPHAREPGLGFAPGVDITIHVLKPGESTRAVRRNANQIEFCIRGCGI
                     VQVGADQIPVGKWDVCTIPSMQGYAHRNHGKDLWVRLTYSNTPLLEKLGAYHAVLASP
                     QETYAAEARTAVPDTEKRYTRANAPDIPVGDHGARMRGYEFLTDIAVRPNVALHWPWR
                     EIEQHVSRKAGDGKRGIMLLYNPATERRNGTTHTFFATISSTPVGAAPRPRGRGHKHS
                     SVAINYHFEGAGQSTVDGTTIEWRAGDLLLSAPSWSEHAHYIHPQGASVLTVQDHPLH
                     IGMESLIWQERMDGPILTLGSEAGQTGYVGPRTAGA"
     gene            complement(22455..24743)
                     /locus_tag="VGC69_03755"
     CDS             complement(22455..24743)
                     /locus_tag="VGC69_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0294433.1"
                     /translation="MSARPQDLYNALYAAQGALLVVRELPATAKAAPGQFGGAFGAAP
                     ANPEIFIAVLADGRIVACNGHVDLGTGIRTALAQVAAEELDVAWGRLQMVLGHTEAVP
                     NQGPTVASATIQVSAEPLRRAAAQARAHLLGLAAQAWGAPVDALAVEDGVVRAPDGRR
                     AAYADLLQGRRDVLALDLKAPIKPVSEHRLVGRAMPRVDIPAKVTGELTYVHDVRVPG
                     MLHGRVVRPPYGGRDTGDFIGHTLVSVDASSIAHLPGIVRVVVIQDFVGVVAEREEQA
                     IQAAQALKVAWREPPAQDMSSFERAIRRQPYVERLLTDQGDVEAALESPRTLRRTYVW
                     PYQLHGSIGPSCSVADYGAQGLRVWSGTQNPHTLRADLATLFDLSEDRVEVIRLEAAG
                     CYGRNCADDVGADAALLSRAVGRPVRLQLSRNQEHVWEPKGTAQLIDVRGVADEAGAP
                     LAYDVCINYPSNEAPVLALLLTGKLPAAPRIKEQGDRTATPPYRFPRLRAMCHDMPPI
                     VRASWLRGVSALPNIFAHDCFLDELAEQAGADRCDYRAQHVEDPRAAELIREVAARAG
                     WRQGARGSRGVPDAQGLLHGRGMSYARYVHGKYPGTGAGMAAWVADLVVEVASGRIKV
                     ERIFVAHDAGMMVNPAGVRHQVHGNIIQSLSRVLMEVVEFDARGVRNREWGAYPILRF
                     ADLPEIDAYLMPRQDQPALGAGESASVPGPAAVANALFDATGCRFYAPPFTPETVRRA
                     LQNRAAQQANEPAGSAREEVPA"
     gene            24872..25390
                     /locus_tag="VGC69_03760"
     CDS             24872..25390
                     /locus_tag="VGC69_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0294434.1"
                     /translation="MNRPADPTPLTLEVNGKTHVVPAADPATPLLYILRNDLALNGPK
                     YGCGLGECGACTVLVDGVAARSCVIPAAGVVGRRITTLEGLGGIEAPGPVQQAFIECQ
                     AAQCGYCINGMIMTTEALLRRVPAPTEQQIRDELRHNLCRCGTHLEILQAVHRAVQIK
                     AESAPQPASTAR"
     gene            25427..28285
                     /locus_tag="VGC69_03765"
     CDS             25427..28285
                     /locus_tag="VGC69_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0294435.1"
                     /translation="MQDLPVFQPLPTDALAGLVLRPPGARWDGRRYCGPSLAALDTET
                     LEHMPGFIRLVAQGSFVGLAGSTPTLVLQAAARLQAQWQAAPDSAAMGGGHTLSHEYR
                     WTGQAPGASGEHQVVVWAHAAGASVWTPHAPGPRLRRELAALLGCPETAVQVHRLPSS
                     GALPDPLALIDAAADAALLSRACATPVRIAVGKPQDQALPALKLSADLDERGHIADLY
                     GPMLLERPSLARLLAGGASGSDQDAPGYALALLPAADAAQALPGAPLGQSLPAWVFAQ
                     ESFIDEAAHATGADPIEYRLEHIDDPRARALIEDVSQRAGWTPRISDTPDGAGRGFAY
                     ASVLDATQDPPRQVWAAWVADVKVDPRTGLIDVTRLVVGHDSEAGRTELDAAALDREL
                     LAATRQLLQAPAPRFDDWATPLADDGTAGPESTPESTSLTLRPLIQHALAEGSRQLAA
                     PGQGLAWTPALALPAAAAIANAVFDATGVRLRETPFDGAAVRRQLGAPAAPEPRKRPL
                     ARAAAWLGATAAGVAGATAMLWPWPAAIAPIVQPDIPLYSAAAIERGRLVAQAGDCAV
                     CHTAPSGRENAGGLALDTPFGTIYTSNITPDPQHGIGNWSYTAFERAMREGIHRDGRR
                     LYPAFPYTAYAKLSDADMQALYAYLMTRPAVAEPAPATKLAFPYNARPLLAGWNMLFH
                     DDSPFQPDPTQSTQWNRGAYLVLGAGHCGACHTPRNALGAEKNGNDFLSGGLAEGWQA
                     PALNTLNQAGVPWTEQDLTAYLRTGYSPLHGVAAGPMAPVIHGLAQLPEQDVKAMAVY
                     LLSLNQGAQPAAHEAAPQPAPSPAAQALALESRSAPQLRLFDGYAKGERLYENACAVC
                     HEAGQGPTLHGVKPSLALNTNLQAGQPDNLIQVILNGITQPADPALGYMPGFRDSFDD
                     QQINDLVAYLRVRFGNGQPAWTGLQESVARIRAQPVAH"
BASE COUNT         5186 a         9583 c         8876 g         4680 t
ORIGIN      
        1 ccccgacagc gacgacctgc tctcgggcgt ggtgctctcg gccagcccca ccgaggtcaa
       61 ggtggcgcgc agctcgacgg aaaccatcac catcaacgac aagaaggccc tgtcggtcat
      121 cgcccgggcg ctgagcagca aggccaagtc cgaagtgcgc atcacgcgcg gctcggtggt
      181 ctatgtgcac aagaacggcg gcacctggga aatcatcaac atgcccacgc tgcaagccgc
      241 catggtgtcg ctcgcgccgc aggacggctc cattcgcgcc atgatcggcg gcttcgattt
      301 ccagcgcggc agcttcaacc gcgcggtgca ggcctggcgc cagccgggct ccaacatcaa
      361 gcccttcatc tacgcctccg cgctggagcg cggcctgtcg acgaccaccg aaatctccga
      421 ccagcccttc ttcatgagcg ccgcgcagac cggctcgaag gactgggcgc ccaggaacga
      481 cggggacgag tacgaacccg agctgaccct gcgccagggg ctctacaaat ccaagaacat
      541 ggtgtcgatc cgcatcctgc aggccatcac gccgcagtat gcgcaaaact acctcacccg
      601 cttcggcttc gaggccgacc gctgggcgcc cgtgctgccc atggccctgg gcacgggcag
      661 cgccacgccg ctgcaggtgg ccggcgccta tacggtgttc gccaacggcg gctaccgcgt
      721 cccgcctttc ctgatcgacc acgtcaccga ccgcacgggc aaggtgctga tgcaggccca
      781 gcccatccgg gccggcgacg aaacggcccg cgtcatcgac gcgcgcaccg cctggctgat
      841 ggacgacgtc ctgcgcggcg tcgccaccta cggcaccggc gcgcgcgcgc atgccgtgct
      901 caagcgcaac gacgtggccg gcaagaccgg caccaccaac ggcgcggccg acatctggtt
      961 ctcgggcttc acgcccagcc tgctcaccac ggtctggatg ggctttgacc agccgcgccc
     1021 gctgggcacg cacgaattcg gcagcggcgt ggccctgtcg acctggctcg gctatatgca
     1081 gcccatgctc gccaacgtgc cgcagacccc gccgccgccc atgccgcaag gcctgatcca
     1141 ggccaacggc gactactact tctcggaatt cccgccaggc caggccgtgg cgtcactggg
     1201 cctgtccggc agcgacggca acggcgtccc cgtcaccaac gacagcgatg tgctcggcgg
     1261 cttgctggac aaggtatggt cgagcctcaa ggaaggcccc gccggcaacc gtatcgagac
     1321 gcaaaaggcg accccttgac gaaggcggct gccgcgcctg cgcggcagcc gcccgcctac
     1381 gccatggcga agcgctcggg gtccgggccc aggcggcccg cttcgggaat gtcgtcgatg
     1441 gcggccatgt cctcggccga cagcgtgaaa tcgaacacgt cgatgttctc gcggatgcgg
     1501 gccggcgtga ccgacttggg gatcaccacc aggccatgct gcaggtgcca gcgtatcatg
     1561 acctgcgccg gcgtcttgcc atgctgcccg gcgatcgtgg caagcacggg gtgatccatc
     1621 atcttgccct gccccaaggg gctccaggac tcggtgacga tgccgtgctt ggcatgaaaa
     1681 acccgcaagt ccacctgcgt gaaggccgga tgcagttcga tctggttgac ggccggcacc
     1741 acgcccgtgc cttcgatgag ttgcctcaaa ttggcctgcg tgaaattcga cacgccgatc
     1801 gagcgcgccc ggccgtcggc ccgcatttca atcatggcgc gccaggcatc catgaagcgc
     1861 tcgctgcccg ccacgggcca gtggatcagg tacaagtcca cataagccag gcccagcttg
     1921 gtgaggcttt cgtccatggc cttgtgggcc tcgtcgtagc catgacggct gttccatagc
     1981 ttggtggtga tgaagagatc cttgcgcgcc aggcgcgatg cgcgcaggcc cgagcccacg
     2041 ccctcttcat tgccatagat ggccgccgtg tcgacggccc gataacctgc ggccaaggcc
     2101 gcctgcacgg cctcgttggc ctcgtcattg ggcacctgcc acacacccag gccaaactga
     2161 ggtatgcggt tgccgtcatt caacgcgata gtcggaacct gtgccatacg acctccttgc
     2221 gtaatgcaaa ctgtgggaat gatagtcgct caatccaggc tcgcctatga ttcacgacgc
     2281 atcgttacgt tacgaacagg tgcgatgtta cgtaacgaag cgccgtttgc ctattgtttg
     2341 ctttttgtca ggtccacagg gccgccggat ccgccatgcg caaggccaac tgtcccttgt
     2401 tggtaatgcg aaagccgctg ccctgcggct cgatgcagcg cagtatctgc aacgcctcgg
     2461 caatgtgcgc gggcacgggc tcggcttcgc cgcccggttc ggcgatcacg cccagccagt
     2521 aggccagctc cccctgccgt atgccgggat ggttcatagc ggccccttga aaatgaaaaa
     2581 agccccggcg aagatcaggc cgaagcccac cacatgattg atggtgaacg attccttcaa
     2641 gtaaaacacg gaaaaaatcg agaacaccac cagggtaatg acttcctgca tggtcttcaa
     2701 ctcggcggtc gagtacaccc ggtggccgat gcgattggcg ggcacggcca ggctgtattc
     2761 cgccagcgca atgcaccagc tgaccagcac caccttgacg agagaggtgc tgggaaactt
     2821 cagatgcccg taccaggcga tcgtcatgaa gatgttggac agggccagca agccgatggg
     2881 cagcagtttt tccagggata aaggcacggc ggccctctag ctaaacaagc aagacggctg
     2941 gtgcgtcgca ccagccgggg ccctcatcat aatggatcgc cgttgatgca gccgcctagg
     3001 gctgggatgc gccctgctgc cgccccacgc cgcgcacgaa gtaaacgcgc atggcctgcg
     3061 cccgcccctg cgcatcgctc acgcgcagcg tcgcctgcct cggcacgaag ccatcgggca
     3121 atggcacggt gccctgcagg ggcgcaaagc gctccagcgt gaacgacgcg ggctgggaga
     3181 cgatcgtgtc ggcccggcca ttgcgggtgc ccgccagctc gaccacgacc tggccctgga
     3241 aggcggagag cttgtcggcc tgctcccgca tgatgagcgc acggtagccc accccgccat
     3301 cgcgtgaaac gaattcgccc cagcgcacgc cgatgttgcc gccgcgcgga tcggctggta
     3361 ccgcatcctg caggatctgc agcgtctgcg tcaacgtggc gatgcgctcg ttggcctggg
     3421 caaggtcgcc cgttgccttt tcctgcgtcg actggctctg ctcgcgctgg ctctgggcct
     3481 cggtcaactg agcctgcagg ctctggcgct ccagcgtaac gctgttgagg tcgctgagca
     3541 gcttctgcga cgcctcgacc gtcagctgct gcggcccgta gttatgctgg aagaacagca
     3601 ggccgcccgc gcccagcacg atgccgacgc acagcagcac cagccagcgc gggacacggc
     3661 ggtcgcgccg tcctggttcg aaagtcgaag gcttgaaaac ggttttgcgc gagctcccaa
     3721 acatccccat cttatggatc catccctgca tgattggcca agacagtctt gccgcctaac
     3781 cggaaaggat atcgtcagat gagccctggg ccacgctttt acaatattcg gttgctcaag
     3841 aagacactat gaaaagccat gtatccggca agcgggggcc acagcaagag tcgcggggtc
     3901 agtggtcgct tgaaagcgta acaaaagaac aagggcttac catcttgcga cagcaagccc
     3961 ttgaggtatt gcgggtcggg tgagccgcca tcctcgacag ggtcagactg tcgcgatggg
     4021 cgtcaccggc gaaccgactg cgcctggcag gcgcaaggga ggcgcgctga ggaaaaaacg
     4081 gaagcgttta tggtggcgaa gatgctccgc aagcggggtc aaatgccaca actcccccaa
     4141 tggcacgcct agcttgaaca ggcaatgcac gtgcagtgga accatgggcc gcggagtcag
     4201 gccgatgacc gggataaaac cttccaccgc gtagttatcc gcgacgatgg ctgctacgcc
     4261 actggcgccg acccactgca gcagccgttc atccgtacca tcgagtaccg cgcaggcatt
     4321 gttgagcacg gataggtcgg gcttgccttc catggagagc aagcgttcgg caaacccggt
     4381 gtgcaagcaa agaatatcgc cttcttcaac cgccaggcca tcagcttcga agatttccat
     4441 cagggtgtcg tagccgactc gccggcgctg agcgccgaga tgcgcatgca agtccaccat
     4501 cacgccccgc ccctgaatgg cggcggcggc catcgtctgg atgccaagcg gcatggtggc
     4561 cttgccgggt tcgcccggcg cgacgatgtc cgtctcggca taaccgttgt aataggtcat
     4621 ccgctcttgc cccgtgccat gggcatcgaa acggcaacct atgtgggcca ggctgtccca
     4681 ttgcgtggag tattgcgtgc acagggtaac gatgtcgtcg ttgccgcagt cgcagaacgg
     4741 gccttcttga ccgaacgcat aattagcgaa tggacggccc gaacgcatgg ttggcgcgag
     4801 ttgcggcggt ttgcgatgcg gcgcgagcac cgagccgccg ggatagtcca gcggcaggct
     4861 cagacagaag cgttcgccag tcaggatttc gcgcgaggcc ctcagcaccc gggcgggagt
     4921 cagcagattc aatcgcccaa gctggtcgtc ctcgccccaa tctccccacg tcgacccttc
     4981 gggccggtgc gtccagcgtt tcatcacatc tcctatttga cgatcatcgc ggtagacaac
     5041 cagggcacgc aggccacgat gagaacaccg acagccagta ccgccatata gccccagatg
     5101 gctttcaggc ctgcattggg atcgatacgg ctgatggcgc aagctgtgta atagccgatg
     5161 cccatgggcg gcgagaacaa gcccaacccc atggagagga tgatcaccat cgcgtaatgg
     5221 atttcgttga cgcccagctc cttggcgatg ggaaatagca atggcgcgaa aagcacgacg
     5281 gcgggaatcc cctccaggac agaccccaat aaaacgaata gcagaatgga ggcggcaaga
     5341 aaggtggctg aaccgcccgg gatcgcgctc atcatttgcg ccagctgttg ggaaaagccc
     5401 gattgggtga ttgcccagcc cattgccgta gccgtaccca tgatgaagag cacggcgcct
     5461 gaaagcgtcg cggtcttggc gagcgacggg cccagcttgc gccattccaa gtggcggtag
     5521 agtatcgctc cgacgatcaa acaataggcg atgcctatgg tcgagacctc ggtggccgtc
     5581 gcgataccct cgacaaccgc atagcggatg acgaaaggca gcgctatggc aggtagcgcg
     5641 cgcacgaaga tgcgacgcac atgggcaaaa ccggcgcgcg gggaaacgcc cgtagtctgg
     5701 cggcgcgcct tgtagcccac atagacgcac aacatgagcc ccagcaataa actcggaagc
     5761 agaccgccgg caaacagtgc ggcaaccgat acgccggtca ccgaaccgat cgtgatcagg
     5821 accaggcttg gaggcacggt ttccgtctgg gcgccggtag ccgacagcag cgccaccagt
     5881 tctcccggcg gagtgcccag tttgcgcatt tccggaaaaa gaatgggaac cactgccgcc
     5941 atgtcggcag ccttggcgcc ggaaataccc gatacgagat agatggcgcc gatcagcaca
     6001 taggacaggc cgccacgcac gtgaccgagc aacgacgcca gaaaatcgat gatggccttg
     6061 gccatccccg acaattcaag caacagtccc aaaaagatga acagcggcac tgccagcaga
     6121 atcaagtccg tcatgccggc gctcatgcgc attacccata ccaggctggg gacatgcgtg
     6181 gcgaacgtga catagccgat gctggccagg ccgaaggcga agccgatcgg cgtgcccagc
     6241 accagggcga tgacgaccac gccgacgaag aagatgagca gattccattg tccaaggccg
     6301 gtgaaagcgt cgcgcgcata ccagacgcca acgcccagca acgccaatac gatggccgag
     6361 cccgctaccc ggcgcgccgc gacgggttcg ctgaacagcc gcaggatttc aaacaaaccc
     6421 atgagaacga cgcctgccac gacggctctg taacgccagg acaagggcaa acccagcgcc
     6481 ggcgagtgga tcatggcttg cttgtctgcc aggctgaggg cgggcagcgc gagcgcgcac
     6541 aggaagacaa gacaggccag gcgcacgaaa gcgtcaagcc ggtcgcgcca gacttgcggc
     6601 agccgatcca ccaccgccgt catcttcata tgctcatcgg cgcgataggc caataccgcg
     6661 cccagcatgg ccagccagag aaacagatac tcggccagct cgtcgctcca tataagcggc
     6721 atctggaata cgtagcgcgc aacgatgccg gcaaatagca aggcggtttc caccacgacc
     6781 aggatggagg cgatgatgtt gactatcgtg accaagccgc gatcgatacg ccgcacaatg
     6841 cgaaggccgt ggcttccggc gctccgcgat gtgctggcgg gcaacggatt agcggctttc
     6901 atgccagcct cttgtccagg cctttttcca gcaaggtcca cgtctgcgaa ccaaatttcc
     6961 cctgccactg cttgtagaag ccggccgcgc gcagtgtcgc ctggaaggct tcggggtccg
     7021 ggcgattgaa ggtcatgccc ttcttttcca ggatgccttg cagcgtgtcg ttggcaaccg
     7081 tggagtcctt gcgctgttgc aaggttgctt ccgcgaagct gtcattgacg attttccgca
     7141 gcgggggggg caggctggcc aacgagcccg gattgaccag catccagaag ccatcccaca
     7201 tatgctcggt gagcgagcaa tatttctgca cctcgtagag cttggacgcc tcgacgctga
     7261 tgagcggtgt ttcctgtccg tcgacgacgt gcgtttgcaa cgcggtatag acctcggccc
     7321 atccgatgct ggcaggcaaa gtcccgagcg ccgagaacag agaggtccac agcgggctga
     7381 caggcacccg gatcttgaag tttttcaagt cgtcgggcgt gttgatcgga cggttgctgg
     7441 tcgtgatatc gcgaaaaccg ccattgaata tgtacggcag cgcctgcaat tccgccttct
     7501 cgatggactg ccggatatgg ttacccaggt ctccgtccat gatcggccaa acttggtcga
     7561 tgctcgtgaa tgcgaaaccg acgccgctga tacccgccgt cggaatgagg tcggccagca
     7621 agatgcccga catggtgaaa aagtcgatag cgcccacgcg cagctgggtc agcatgtcct
     7681 gatccgatcc cagctggcta ttgggaaaca gatcgatgcg gatctggccg ctggagcgtt
     7741 cggcgatcat tttggccgca ttcgccacgc cgatggtcaa cggatggcgg tcattgacat
     7801 tgcctgcata tttgaacgtg tattgggccg tacttgcctt ggcggtatgc acccagggga
     7861 tggcgccagc cgccaggccc atgccgagaa tcttgcgtcg cgttaccatt gatgtctcct
     7921 ttcttataaa aatttaggta aatcgagaga gggaatccag ctatgtcagc gggcggcgca
     7981 tgccttgcct ccgtccaccg gcatgatcac gcccgtgatg tatcgggcct cgtcgctggc
     8041 caggaacaat gatgcatagg caacgtccca ggcgtcgccc atgtgcccca tgggcgaggc
     8101 agcgtgccgc ttggccagca tctcgtcccg attggcgtaa ttgcctgcaa tctgcgtgtc
     8161 gatgagcggg gtatacatga cgcctggcag caccgcattg acacgtatgt tgtgcggtgc
     8221 atagttgacc gccagactgc gcgtcagatg attgaccgcc gccttggtcg attgataggc
     8281 gggatagggg tactcgttga tctggatcga ggccagcgag gaaatattga cgatggcgcc
     8341 cccgccctgg tttcgcataa cgggcagaac ctgccggcag gcgtgcacga cgctgaacag
     8401 attgatttcc accgccttct gccaccgctc catgggcaat gattcgatat cacccatgga
     8461 agtcatgccc acgttgttgt gcaatatgtc gatgcggcca tgagcctctc gcactgcgcg
     8521 taccacgcgg gcaatgtctg cttcgatcgt gacgtcggcg gcatgggccg tggcctgtcc
     8581 gcccacgccg gagatgagat cggcagtctc ttgcgcggcc ggcaagccga tatcgacaca
     8641 gacgacctgc gcaccctgcc gggcatacac cacggccgcg gctttgccgt tgctccagcc
     8701 cggcgcgctg ctgcccgcgc cgaacaccag cgccaccttg ccttcgagcc gattgcctgc
     8761 gggcacctcg cgaatatcgg attggggatt aagcaacgcg atctcccttc ttcattttca
     8821 ccgcgcggtt gtccacgccg ggcagcattt tcgaggcatc cctcgtgacc acgacatcga
     8881 tgaccgtcgg tcccttgagc gtcatggcct tttgcaacgt cgcgcggatg tcctccggct
     8941 tttccaggcg caggccgttc acgcccatgg cttcggcgat cttggcgtaa ttggtctcgg
     9001 ccaggtcgga agcatggtag ccctccggtc cgtagagcaa atgctgcagc gccttgatat
     9061 agcccgacgc agcgttgttg acgaccagga cggaaaaatt caaaccgagc cggcgtgctg
     9121 tttccagttc acccagcatc atgttgaagc ctccgtcgcc ggtaagcgcg aatacgggca
     9181 cgctgggttg cgccaaggcc gcgcccatgg cgccgggcaa gccgtagccg atggaggcaa
     9241 aaccgcgatc agggatgaag cggcgtccag cggccttggt gtcaaacaaa aggccgcccc
     9301 aatgcgcggc aaaaccgccg tcggcgatca ggatgccgtc ctccggcaga acatggttca
     9361 gttccgtaat caggcgcgcc atattgatcg gcacttcctc ggaatgaagc cggggaccga
     9421 cttcctcccg ccacttggcc atgcgagccg gcacttgcgc cgcccagctg cctagccggg
     9481 cagccatttc cgcctgttgg gggcgaataa gcgtatccag ttcttccagc gtcgcacgca
     9541 cgtcgcccca cagcgctacg gtgggttcca gcgtgcgccc gaattcctcg gcaacattgt
     9601 cgatgtggat aagacacttg ccaggaccag gaacggtata gcgcttggtg gctatctccc
     9661 cgagcttgca tccggcaacg atgacgacat ccgcctcatc gatcaacccg ttggcaatgc
     9721 gatcgtagcg gccaaatagg cctgcactga gcgggtcggt gcaggcgatg gcgcctttac
     9781 cagtcaaagt gtgcgcgacg ggaatattga atgcccgcga aaatgccgtc agcgcctcat
     9841 gggcattgga taggtgcacg ccgccgccgg ccagcagaat gggcttgcgg gccccgcgca
     9901 gcaacgccgc cgccgccgcc aggctctgca catcgggacg cacccgcaag gccggcgcgg
     9961 cttggtaatg cgcggcgatc gagaattccg cctcgtcgaa ttcgcaagta tcgtgcgcga
    10021 tgtcttccgg gacatcgacg actacgggac caggtctgcc gctggtcgct acggcgaagg
    10081 ctcgtttcat gagttccggg atccggctga tcttctctat ccggatgacc gccttgcagg
    10141 ctggcttcag gattgcaatc tgatcggact cctgggtcat gttcttccag gagtgatcgc
    10201 gatgagtatc gcccacgatg acgaccatgg gggtgccggc attgagcgct tccaccagtc
    10261 cggtcaccag attggtcgcg ccgggaccga gcgtggcgtc ggcggctccg acgcgtccgt
    10321 tcgatgcttt gctgtaggca tcggcgatga agacggcggc gcgctcgtcg ttgatgagat
    10381 gatgcggcat attcaaccgg cgggtcgcat cgtaaaaggg caaggtctga aacccgccca
    10441 tgccgaagat cgggccgatg ttggccgctt ggaatatgcg ggcgatggcc tcgccgcctg
    10501 tcatttctaa tttcatcgtg ttgtctcttg ttgtcgtatg gggcgcatcg atcgcgccaa
    10561 cttagttaat gggaagagag gcgaatcttg cggaaaattg cccggccaat actgcggctc
    10621 gggcctgcat gtcgggtacg agctgcagcc ggcgcttttc gtccaatcga gagctgatgg
    10681 cggctatcga cagagccgcg atgacttcct gctgctcatt caagatggga accgcgatac
    10741 cgatcatgct gtcgatgatg ccgccatcgt tgatcgcata gccgtttgtc cgggcgttgg
    10801 ccatcagttc acggaccagc ggaatcgata tgcgcgggta gctcgcgatc cactgctcat
    10861 tgatttccag gtagcgctcc cgccacgccg gcggcgccca ggcgagcagc gccaacgctc
    10921 ccgcccccac gcctagcggc cggcggtcat agaggttcag cgtcagcgtg cgtatgggaa
    10981 aaccgccttc gtggcgagcc acgcaaaccg cgccggaccc ttcgcgcacc gacagaaaaa
    11041 ccgtatcgcc cgtttcctcg gccagggcca tgagcgttgg ccgtgccagc agcgcagcca
    11101 ggtattgcca ggcgtgtgcg cctcggcctg ccagcctgac gccgaggtga aaggttttgc
    11161 tgtcggcatc gctatagagc catccgaccg cggtcaacgc gtgggccagg cggtgggtcg
    11221 tggtcttgga caactgggtg cgctccacga gctcggccag gttcaacccg cgatcgccag
    11281 cctccgaaag ggcatcgacg agcgctgtcg cccgctccag gatgcgcacc gagggcgcca
    11341 gcgaatcggc cagtttggta ccggccaaat cgtcttcaag gtttttttgt tccatagaca
    11401 tggttttaat ttatcatctt tgtggaacga actgcaattg tgcaatgcgt caaatccata
    11461 acgagggaaa ttccagccat gccggaccta ttgccaattg ccctggcgga caccgccacg
    11521 acattgcatg ccaccgaggc aggcaaggtt gtggtgtgcg gatcccatgg agcgctttac
    11581 cctgccgcgc tgctattggc ggccaaggtc cgagggagca tcctgtatga cgcggggggg
    11641 caggggcaga ccggcctggc tgtgcaggaa ggcctggatt tactggaacg gcatgggctg
    11701 ccgggcgcac tgtgctcggt gcatgcgagc cgcatcggcg atgcggctga tgcctggacg
    11761 cgaggaatga tctcgtcctg caacggcctt gccgctcgcc tgggcgtaca gcccggcatg
    11821 accgtgcgtg acgctgccga acggctcacc catgcctcct gggacgccgc cgccgtgcct
    11881 accgtggcgc cgccacggga gactcggtgc gtcatcggag taggagaact gcatgtcgtc
    11941 ctggtcgatt ccgcttctct ggtcgagcct caagacgcgg gaaagatcgt catcacgggc
    12001 tcgcatggcg cgctggtagg caacgacccg gccaaagcgc tggccgtgga tgccgccgcg
    12061 gcagtttttc acgacgcggg catgggcata gggcaggcag ggatacaacg attgggcgca
    12121 ctggcgcggc gcggcatcgc ggcggtcacc gtggccgccg attccgcgcc catcggctgt
    12181 gcgaaggcca tctgggatac cgggataatc tccgccgcca acgaaaccgc ccgccaaacc
    12241 ggagcgtcga acgggcaatc gttgcaggcc tgggtatggc agcttggcga tcttgaatcg
    12301 cgcggccaag ccggtcgtag atgaacgacg cagccctaaa ggaaaagggc aaaagataag
    12361 ggtgttagtg accatgtcac taacaccctc ttgtactact ggtcgggacg gcgggattcg
    12421 aactcgcgac cccttgcacc ccatgcaagt gcgctaccag gctgcgctac gccccgaaag
    12481 accgagacta tatcatagat aaataccgtt gctcaaatcc ccggcgcatc cccgacaagg
    12541 cggatggccg cgcccgccgt cagcgggatc tccaggccca gccccgcctc ccgggcgcgc
    12601 tggtaggcca gcatggcgac cgacaggtcc gagataccca ttcccatcga cttgaacagc
    12661 gtcaggtccg tatcgcccgc gatacctttg ccgcccgcga tgacctcgct caagggatgc
    12721 acgcgggccc agtcgcccgc gccggcgcct tggtcgaaat agtcgatgaa ctcacgcgat
    12781 gccttgcggg tcgccgacac gtcgtcgacg gcgatgaagc cgacccggtt gaagacatcc
    12841 tgatggaact cggcgctggc cggcaaaatc gcgcccacgg cattgagatg cgtccccttg
    12901 tccagggcct tggcctccag gaagggcgtg cgcgcgcgcg tcacggtcgt cacgatgggg
    12961 gcgcccgcgg tggcttcccg caaggtcgcc gcctcgacga cgtcgaaatc gaattgcgcg
    13021 cgcaaggtct cgcaaaatgc cttgcgcttt tccggcgtcg gactccagac gcgcaagcgg
    13081 gcaagcggcc ggaccagatg cacggcggcc acctgcgcca gcgcctggcg gcctgaaccg
    13141 atcagggcca tgtcgtcgac gccgggccgg gtcagccact tggtccccag ccccgtcatg
    13201 gccgacgtgc gcaactgccc caggacattc gcttccatca gcgccagcag gcgaccgttc
    13261 accgcgtcga aaagcacgta cagggccttg gcgccctgcg gggtattgat ccagttcttg
    13321 tagccgcaaa accccacgcc gggcagcgcc gaccccaggg aatgcatgga cgaggcgctg
    13381 ccaaagctcc ccaacgcctt gtcgatattg aagccgtcgc cgctgcccaa ggcccgcagg
    13441 ccgtcctcga gggcgccgat ggcgtccttc aggctgacaa gccccgatac gtcgttttca
    13501 ctcaaccaga ttgccgatgc actcattacg tctcccgccg tttaaatatc gtcttgaacc
    13561 ctgttgcgca acacgccgag cctggaaatt tccacttcga agacatcgcc ggcgcgcata
    13621 tagcgcggcg gcttgcggcc caggcccacg cctgccggcg taccggtgga aatgacatcc
    13681 ccgggcaaga gctcggtgaa cgtcgacaaa taggcgatca gttcggccac cgaaaaaatc
    13741 atgttggcga cgctatcgtg ctgcacgacc tcgccgttca accggccgat gatgtccatg
    13801 ttctcgggcg ggccggcctc gtcgggcgtc accagccagg ggccgaacgc cccggaagca
    13861 tcgaaattct tgccaggcgt cgcctgggcg gaatgccgct gccaatcgcg caccgagttc
    13921 tccgccaggc aggcatagcc cgccacatgc cgcatggcgt cttgctcgga aatccgccgt
    13981 ccctgccggc cgatcacgac ggccaattcc gcctcgtagt cgaacaggtc ggactcgcga
    14041 ggccgcagca ccggctgccc ctgcccggta agcgacgaag cgaagcgcac gaagatcgat
    14101 ggcttggcgg gcacctgcat gccggcttcc tgggcatgca ggtgatagtt cagcccgacg
    14161 cacaaaatct tggccggatc gatgaccggc ggcagcagca ctacgtcggt caaggcggtg
    14221 ctgccgcccc ggttttccgc caggcggccg gcaaggccgg ctatgccctc gccggcaagc
    14281 gcggcgcgca gcgtgggggc cgatggcgag gccagatcga ccaccgcatc gccgacgacg
    14341 gcgccatagg acggccgtgc cgggtccttg cccggatcga gatagctgac cagtttcatt
    14401 atttcctccg cattcgatgg attcccgcca atcgcgcggc gccctctcgg gcgccccgtg
    14461 ccatggcatc aatacacact gggtccgcca gtgcgttgtg gtgtcgtgcc ggccgccttg
    14521 aagcggccgg ccaaggcctc ggcgccccgc gccaggatcg tggtgtcgac gcccaccgcg
    14581 gtgaaggtcg tgcccagttc gatatagcgc cgggccaggg cttcatcgcc gatcaagatg
    14641 cccgcggcct tgccggcccg gccgatgcgg gcgatggcgt cctcgaccgc gcgctggacg
    14701 tcgggatggc cggggttgcc cagatgcccc atgtcggccg ccaggtcgga cgggccgatg
    14761 aatacgccat cgacgcccgc cacgccgcag atgggctcca gttgcgacag cgcagcctgg
    14821 gtttccacct gcacgagcac gcagatctgg tcattggccc attgcaggta atcgccgtcc
    14881 cggttccacc gggatgcccg ggccagcgcc gaccccacgc cccgcacgcc cgcgggggga
    14941 tagcgggtcg cctcgacggc ggcgcgggcc tgcgccgcgt cctgcaccat gggcaccagc
    15001 agcgtctggg cgcctatatc gagcaggcgc ttgatttcca ccgtgtcgtt ccaggcgcag
    15061 cgcaccaccg ggtgggacgg cgaagacgcc agcgcctgga gctgcgccag cgtcgtggtc
    15121 agggtgttcg gcgcatgttc gccatcgatc agcagccagt cgaaaccggc cccgccgagc
    15181 atctcggccg tgtagggttg cgccaggccc agccacaagc ctatctggga ctgcccgctg
    15241 gccagggcgc gcttgaagag attctccttc accatggcgt cacccgaaat ggcagctgat
    15301 ggagccgagc gaaccgtagt ccacgtggaa ggagtcgcct tgcttggcaa agaccggccg
    15361 cgtgaacgac ccgccgagta tgacttgatc cttttccagg gccacgccga aggcggccaa
    15421 tttattggcg agccagacca cgccattggc ggggtggttg aggacgccgg cggcgatgcc
    15481 ggtttcctcg atcaccgcgt tctgcatgag gatggccccg acccagcgca gatcgatttc
    15541 gccgatgcgc accggccgtc cgcccaggac gacgcccgca tcggccgcat tatcggcaat
    15601 cgtgtcgaag accttgcgcg tgcgctgggt ctcggggtcc accatctggg tgcgggaatc
    15661 gatgatttcc aaggcgggaa ccacatagcg caccgaatcc agcacatcga acaaggtaac
    15721 gccgggtccg gccaatcgct tttcaatgat gaaggcaagc tccacttcga tgcgcggcgc
    15781 aatgaaccgg tcggtgggaa tcgtgcagcc gtcataaaaa aacatgtctt ccagcaaggc
    15841 gccgtaatcg ggctcgtcga tgccgaccgc ggtctgcatg gcccgcgacg tcagccctat
    15901 cttgtgcccc ttcagcgcat tgccgcccgc gatcttcatc gccacccatt cgcgctggat
    15961 ggcataggcg tcgtcgatgg tgatcccggg gtgccgcagc gatacctgcc tgatctgctg
    16021 gcggcttttc tccgcatcga agaattcgcg cgccagcgac ttgatgagtg catcatccaa
    16081 caaaatgtgc tccttgttca catgcagttg cgggaaacag gccccgcgtt attcggcggg
    16141 cagaaagcgc cggctatgct tgcgccgcac gcgccaggcg ccgtcttcca ggatgaggtc
    16201 gtcggtgctt tcgcgcacgc ccagcatctt cggctcgctc tcccggccct gcgcatcgac
    16261 cgtcgtttca taggcgatga ggtaatagcg gacgcgggcg gtttgcggcg tcgccgcttc
    16321 cacccacagg ttggccaccg tatgggcggt ccgccttttg ggatcgcgct tctccagggc
    16381 ctgcagcacc gcctgcgggc cgaccagttc ggcgccctgg cgatgccaga cgccctccct
    16441 ggccatcaag gcggcgacgc cctggttgtc gcgctggtcc agtccaagaa aaaagcggta
    16501 tacggccgcg ctgaccgcat tgagatcgac ttccgtcatg atcgtgtccc tgttgcgagt
    16561 aaaccggctc atgccccgac ttcgcgcggg ccctgatagc cggcctgccc ccgttcggcg
    16621 ccgagcgcat agatcgggcc atcgatcttt tcctgccaaa gcagcgaccc cagggcgatg
    16681 tgcatcggat gttcctgaat cgtcaggact tcccagccgt cggaaccgaa gtaatgcgca
    16741 tgctcggacc agcccggcgc cgaaaacagg agatcgcccg ctttccattc cacggtcacg
    16801 ccgtcgacga cgctcgttcc gtggccgctc gcgtggtaat tgatcgaggc ggaggtatgg
    16861 cgatgcccgc gctttccggc gaaaggcggc atgcccggca ccgcgccggt ccacgtggcg
    16921 aaaaacgagt tgttcgtccc cgcgcgcttc tcggtcgccg ggttgtaaag cagccagatc
    16981 ggacggtggc cgtcgccgac gcgcatcggc aggtaaggcc gcacgtcctg ccatcgccaa
    17041 agcagcgtct tgttctcgac gacctcgatg tcagtcagga attcgtatcc ccgcagccgg
    17101 gcgccttcgc tgccgatggg catgtccggc gcttcttccc gggagtattt cttcttcgtc
    17161 accgggctca agccgtcttg gcgggtttct tgcgacttga tctcgtcgcc ttcctcggag
    17221 aaaaaaacgg ccagtttttc aagcaggggg gcgttggaat aggtcagccg cacccaaagg
    17281 tccgaccccg tgttcttgta ccgatgcacc cgcatcgcgg ggatattcca cacgtcgaag
    17341 cggccgacct cgtgcgtcgc atcggcggca atgacccggc ccgacccgcg gatgcagatt
    17401 tcgatttcat tggcgttgct gcggtaatcc agcgtttcct ctcccggctt gagcacctgg
    17461 atggccacat ccgtcgaggg cgtcaggctg agcatcccgt cgcttgccgc gggatgcacg
    17521 atcttcgagg cgcggcggcc attggcttgc cgcgcaatgt cggccagccg cgctatctcg
    17581 gcatcgatgt cttcccgcgt gaccagaaac ggcgtggtcg gctggggttt cttgctcggc
    17641 tcgccgctca cgtcggcgaa aaccaggccg cgcaattcgc tgctgcttag cttcattttt
    17701 tatctccttg atgtataggc gcgcatatcg ttggcagtcc gtagtcctcg actacacttc
    17761 cgaaaaaatc agaacactta taaatacgga ggcatccacc atggacttgc atcgcctgga
    17821 tctgaatctc ttgaagatct tcgactcggt ctaccggctg cgcaacctga ccgaggtcgc
    17881 ccatgaagtc agcctcagcc agcccgccat cagccacgcg ctcgggcgcc tgcgcacggc
    17941 attggatgac cggctcttca tccggacggc aacggggctc gaaccgacgg cacgcgccga
    18001 cagcctcgcc attcccgtgc gcaacgcgct cgccatgctt gaagactccc tgctcagctc
    18061 cgaggaattc aagcccgagc actgcgccag ggaattccgg ctgctgctgt ccgacgtcgg
    18121 aaacgtgctc ttcatcccgc ccctgttgag ccatctgcac cgccacgcgc cgcaggtaag
    18181 gctgaagatc atccaggccc cgcggtcgga ctacgaacgg ctgctgcgcg acagggaagc
    18241 cgacatcgcc atcggccact tgaacctgtc cggccagaac atccgaaaag cgccgctgtt
    18301 ccgcgaccgg tgcgtgattc tcagccgcgc ggcgtccacg ggaaaatcca ggcgccccat
    18361 gagccgggaa gacttcgagc aaaggcccca cgtcatcgtc agcccgccgg aagacccggt
    18421 ggaagaaatg ttctccagct tgcacatcgt gcgccgcatc tctctgcaca tacccagcta
    18481 tttcgccttg cccggcatcg tctcgacctc cgagctgctg gccaccgtcc cgtccagaat
    18541 cgccatgaac ctgagcaaga acttctcgtt cgacatgcat gagctgccct tcgcctcgcc
    18601 cgtattcgac gtcaagatgc attggcatct gcggcaggat gccgacgttg cgcagacctg
    18661 gctgcgcaat atctttcacg cgctgttcgc cgaacgggcg gccgagtgag cggcatgggc
    18721 ggccatcggg ccgcaggcct ggctccatca tgagggacgc tccgcccgct ggtccgacgc
    18781 atagcggtac gaccccaccg tgaggatgcc gatggccccc acgacgcagg caacgatagc
    18841 gacttccatc gccaggtggt aattgcccag gcgatcgaac gccgcgccga acagaatggg
    18901 cccgagcagc gtgccgatgg cgaagatgga gatcatcagg ccgtagacca tcccgaacga
    18961 cctcaagccg aaatagcgct tgacgagaaa cggcatgaga tcgccctcgg ccccgatgag
    19021 catgccggtc agcgcggcgc ccaagcccag cagcggatag ggcgtgctgt gggaaaccgt
    19081 cgcgagtatc aagacgccca aggcgccgag caggaaaagg atcgcggaga gaaccctggc
    19141 cgtgatcagg tcgagcagca cgcccgccag caggcgcccg caggtaatgc cgatggcggc
    19201 gaaggaactg ccggcggccg ccagttcgac ggacaagccg ttgtccttga gcatggccgg
    19261 gatgtgcacc atgccgccca ggccaacggc cgcgaccagg gcgatgctga tgctcatgac
    19321 ccagaaccgg ggatgggcga ggctttcgcg ggccgtcatg ccgggcagca cggcctcatc
    19381 ccgcgcgctc ggtttcaccg cgggttccgc ggcggatccg ccgaatcgat agataagata
    19441 cgtggccagg gcgcccaata cggcggccgt ggcggccagc accatatagg tgaattgcca
    19501 gccatgccgc tggatgagcc cgcccgccac cagcggcgcg acggccgcgc cgatgccggc
    19561 gccgaccgag gcaatgccga gcgccaggcc cagcctgcgc tcgaacgaac gcgcgagaat
    19621 gggcagatag ccgaccggcg aagtcgccac gcccagcagg ccgatcagga aggtcttgat
    19681 cgacagggcc gtgacgttgt agggtcccac gctcagcgac agcagaccca gcacgaacag
    19741 caggatggaa acgctgatga ccttgcgaaa cccgtagcga tccaccaggc ggccggcgat
    19801 gggcgaactg atcgccagcc ccagggacgc cgagatgacg cagctggcgg ccgcgccacg
    19861 gccgacgccc agcgtctggg cgatgggcag gaggaagacc ccggtagtcc ccacgaaaag
    19921 cgcggtaaag ccgctcgata gccccagagc cgccgcgatc accagcgcga cgctaccgct
    19981 ggccttggac ttttcttggg tggatgggtg ctcctggacc aacgttgccg atgaatacac
    20041 tccgctcatc tttgtctccg aattgttttt gtaaaacgcc cgccaagtct gcaatgcttg
    20101 ttcgcgtagc gctttgcagg atacaaagcg aggagccaaa agtgcactga aaagtttgca
    20161 tttgcctaat gcatgccgag aatggcgcgt cgcgcgccgg cctatttttt cgagaacagc
    20221 cgggcgaata cgccgcgaaa ccaggcatgg gccagatccg cgtcgcgccg cgcgtgccag
    20281 tacatgcaga tgtccagcgc cggcgccttg aacggcagcg cctgcacctg catgcccgcc
    20341 gcgctgcgca aattgacaac gaccgagtag ggcacgatgg acagcaggtc cgaagaaagg
    20401 atcacgctgg gcagcgcgaa gaaatgcggg atcgtgagga tgacgttcct ggcgatattc
    20461 ttttgcttga gaagctcggt caggggatgg tcgaccgttc ccggcggatc gaccaggacg
    20521 tgcgcgcatc gctcgaattc ccgaaccgtc agcctgggct tgtgcgccgc atccttggcg
    20581 cgcagcacga cccatttgtc catgaataaa tggctgcggc tcaaaccctt gcccatcatg
    20641 ggcaagttgc ccaccgccag gtccgcgtcc ttgtcccgca gcatggcctc gtagcgcgtg
    20701 cgggacgcct gccgcaccga gaccttcacg ccaggcgctt ccttgcgcag gtattgcagc
    20761 aatctcggca ggaagatcag ctctcccaca tcggacagca gcagctggaa ttcccggcgg
    20821 cacgtccggg gatcgaattc ggaactggca ttgaggcttt cctcgatcat ggcgagcgcc
    20881 gccttgatcg gcccgatcag ctcatcggcg cgcgaggtgg gctccaagcc cgccgccgtg
    20941 cgcacaaaaa gcctgtcgcc gacggtgtcg cgcaaccgcg ccaaggcgtg gctgatcgcg
    21001 ggctgcgtca gcgcgacctc ttcggcaacc tgggaaaggt tatgcagccg gtagatgctt
    21061 tcaaatattt tcagcaggtt caggtcgact cggcgcagat ccatgacggt cactcaacag
    21121 gaaaggacta aagcctgaac attaatttca tgcataaatt ccatcaacat tcttcagttc
    21181 atttataaga aagaagctcc taagctgccc atgcagcaga acagtggccg cgttttccaa
    21241 gcgtctgccg cattctgcgg cgcaacagaa ggaggagcaa aaaaatgact gaacaaacat
    21301 tccgcttcgt cgagaagcga cagatatttt ccgaccagaa tgcggtctgg ccgcccatgc
    21361 gcgtgccggc cgaattcata gatcgtgaaa tcgaacgcct ggccgacgcc acgcttgccg
    21421 aaggcgactt gcgcgccgca tccatcgtcc atccccatgc ccgggaaccc ggcctggggt
    21481 tcgctcccgg cgtcgacatt accatccatg tcctcaagcc cggcgaatcc acccgggcgg
    21541 tgcgccgcaa tgcgaatcaa atcgaattct gcatccgcgg ctgcggcatc gtccaggtcg
    21601 gcgccgatca gataccggtc ggcaaatggg acgtctgcac gattccatcc atgcagggct
    21661 atgcgcaccg caaccatggc aaggatctat gggtacgcct gacctattcc aacacgccgc
    21721 tgctggaaaa gctcggcgca taccatgccg tcctggcttc gccgcaggaa acgtatgccg
    21781 cagaggcgag aacggccgtg ccggacacgg aaaagcgcta cacccgggcc aacgcgccgg
    21841 acatccccgt cggcgatcac ggcgcgagaa tgcggggcta tgagtttctc accgacatcg
    21901 cggtccggcc caatgtcgcg ctgcattggc cgtggcggga aatcgagcag catgtcagcc
    21961 gcaaggcggg cgacggcaag cgcggcatca tgctgctcta caacccggcg acggaacggc
    22021 gcaacggcac gacgcatact tttttcgcca cgatttcgtc cacgccggtg ggggccgcgc
    22081 ccaggcccag aggacgcggg cacaagcaca gctccgtcgc catcaattac cacttcgagg
    22141 gcgccgggca aagcaccgtg gacgggacca ccatcgaatg gcgggcgggg gacctgctgc
    22201 tgtcggcgcc gtcatggtcg gaacacgccc actatattca cccgcaaggc gcgagcgtat
    22261 tgaccgtgca ggaccatcct ctgcacatcg gcatggaatc gctgatatgg caggaacgca
    22321 tggacggccc catcttgacg ctgggttccg aggcagggca gaccgggtat gtaggcccgc
    22381 gcaccgcagg cgcctgatgt gccgggcaga tgcgccgtgc tcggcaatgc agccgggcgc
    22441 ggcgcccggt ccgcttaggc cggaacttct tccctggcgc tgccggctgg ttcgtttgcc
    22501 tgctgggcgg cgcggttctg caaagcgcgg cgcacggtct ccggcgtaaa gggcggcgca
    22561 tagaagcggc agcccgtggc gtcgaagagc gcgttggcca ccgcggccgg gcccggcacc
    22621 gaggccgatt cgcccgcgcc cagcgcgggc tggtcctgcc gcggcatgag gtaggcatcg
    22681 atctcgggca gatcggcaaa ccgcaggatg gggtaggcgc cccactcgcg gttgcgcacg
    22741 ccgcgcgcat cgaactccac cacttccatc agcacgcggc tgagcgactg gatgatgttg
    22801 ccgtgcacct ggtgccgcac gccggccggg ttgaccatca tgccggcgtc gtgcgccacg
    22861 aaaatgcgct cgaccttgat gcggccgctg gcgacttcca ccacgaggtc cgcgacccag
    22921 gccgccatgc ccgcgcccgt gccgggatat ttgccatgca catagcgcgc gtatgacatg
    22981 ccgcgcccat gcagcaagcc ctgcgcgtcg ggcacgccgc gcgagccccg ggcgccctgc
    23041 cgccagccgg cgcgcgcggc cacctcgcgg atcagttcgg ccgcgcgcgg gtcttccaca
    23101 tgctgcgcgc ggtagtcgca gcgatccgcg ccggcctgct cggccagctc gtccaggaag
    23161 caatcgtggg caaatatatt gggcagggcc gacacgccgc gcagccagct ggcgcgcacg
    23221 atgggcggca tgtcgtggca catcgcgcgc agcctgggaa agcggtaagg cggcgtcgcg
    23281 gtgcggtcgc cctgctcctt gatgcgcggc gcggccggca gcttgccggt gagcagcagc
    23341 gccagcaccg gcgcctcgtt cgacgggtag ttgatgcaca cgtcgtaggc cagcggcgcg
    23401 cccgcttcgt cggccacgcc gcgcacgtcg atgagctggg ccgtgccctt gggctcccac
    23461 acatgctcct gattgcggct gagctgcagg cgcaccggcc tgcccacggc gcgcgacagc
    23521 aaggccgcgt cggccccgac gtcgtcggcg caattgcggc cgtagcagcc ggccgcttcc
    23581 aggcggatga cttcgacccg gtcctcgctc aggtcgaaca gcgtggcgag atcggcgcgc
    23641 agcgtatgcg ggttctgcgt gcccgaccag acgcgcaggc cctgcgcgcc gtaatcggcc
    23701 accgagcacg aagggccgat ggaaccgtgc agctgatagg gccacacata ggtgcggcgc
    23761 agcgtgcggg gcgactcgag cgcggcctcg acatcgccct ggtccgtcag caggcgctcc
    23821 acataaggct ggcggcggat ggcccgctcg aaactcgaca tgtcctgcgc cggcggctcg
    23881 cgccacgcca ccttgagcgc ctgggccgcc tggatggcct gctcttcgcg ctcggccacc
    23941 acgcccacga aatcctggat caccacgacc cgcacgatgc caggcaggtg cgcaatggat
    24001 gacgcgtcga ccgacaccag ggtatggccg atgaaatccc ccgtgtcgcg tccgccataa
    24061 ggcggccgca ccacgcggcc gtgcagcatg ccgggcacgc gcacgtcatg cacataggtc
    24121 agctcgcccg tgaccttggc cgggatgtcc acgcgcggca tcgcgcgccc caccaggcgg
    24181 tgttccgata cgggtttgat gggcgccttg agatccagcg ccagcacgtc gcggcggcct
    24241 tgcagcaaat cggcataagc cgcgcgccgg ccatcgggcg cccgcaccac gccgtcctcg
    24301 acagccagcg catcgaccgg tgcgccccat gcctgggccg ccaggcccag caggtgggcc
    24361 cgggcctggg ccgccgcgcg gcgcaagggt tcggccgaca cctggatggt ggcgctggcc
    24421 acggtcggcc cctggttggg gacggcctcg gtatggccca gcaccatctg cagcctgccc
    24481 caggccacgt ccagctcttc ggccgcgacc tgcgcgagcg cggtgcgtat gcccgtgccc
    24541 aggtcgacgt ggccgttgca ggccacgatg cggccgtcgg ccagcaccgc gatgaatatc
    24601 tccggattgg ccggcgccgc gccgaaagca ccgccgaact ggcccggcgc cgccttggcc
    24661 gtggccggca gctcgcgcac gaccagcagg gcgccctggg cggcatacag cgcgttgtag
    24721 agatcctggg gccgggcgct catgggcgga ctcggagaca agaaagtata aagatacaag
    24781 aataatactt ttgcgggcgg gtccagcctt ggcaaacccg catcccgccc ggccccggcg
    24841 cgcggcgcgc gcgtatacac ttgcaggcat catgaatcgt cccgccgatc ccacgccctt
    24901 gacgctcgag gtcaacggca aaacccacgt cgtacccgcc gccgacccgg caacgcccct
    24961 gctctacatt ctgcgcaacg acctggcgct caacggcccc aaatacggct gcggcctggg
    25021 cgaatgcggt gcctgcaccg tgctcgtcga cggcgtggcc gcccggtcct gcgtcatccc
    25081 cgcggccggc gtcgtggggc ggcgcatcac cacgctcgag ggcctgggtg gcatcgaggc
    25141 gcccggcccg gtgcagcagg ccttcatcga atgccaggcc gcccagtgcg ggtattgcat
    25201 caacggcatg atcatgacca ccgaagccct gctgcggcgc gtgcccgcgc ccaccgaaca
    25261 gcagatccgc gacgaactgc ggcacaacct gtgccgctgc ggcacgcacc tcgaaatcct
    25321 gcaggccgtg caccgcgccg tgcagatcaa ggccgaatcc gccccgcaac ccgcctccac
    25381 cgcccgctga cgcgcacgcc agccccagac gcaccgatac gcccgcatgc aagacttgcc
    25441 cgttttccag cccctgccta cagatgccct ggccggcctc gtgctgcgcc cacccggcgc
    25501 gcgatgggac ggcaggcggt attgcgggcc gtcgctggcc gcgctcgaca ccgaaacgct
    25561 ggagcacatg ccgggcttca tccgcctggt ggcgcagggc agcttcgtcg gcctggccgg
    25621 ctccacgccc acgctggtgc tgcaggcggc cgcgcgcctg caggcgcagt ggcaggccgc
    25681 gcccgacagc gcggcaatgg gcgggggaca caccctcagc catgaatacc gctggaccgg
    25741 ccaggcgccg ggcgccagcg gcgaacacca ggtcgtggtc tgggcccatg ccgcgggcgc
    25801 cagcgtctgg acgccccatg cgccgggccc gcgcctgcgg cgcgagctgg ccgccctgct
    25861 gggctgcccc gaaacggccg tgcaggtgca tcggctgcct tcctccggcg ccctgcccga
    25921 tcccctggcc ctcatcgatg ccgcggccga tgccgcgctg ctgtcgcgcg cctgcgccac
    25981 gccggtgcgc attgccgtcg gcaagccgca ggaccaggcc ctgcccgccc tgaagctgtc
    26041 cgccgacctc gacgagcgcg gccatatcgc agacctgtac ggccccatgc tgctggagcg
    26101 tcccagcctg gcccgcctgc ttgccggcgg cgcatccggc agcgaccagg acgcgccggg
    26161 ctatgccctg gccttgctgc cggccgccga cgccgcgcaa gccctgcccg gcgcgccgct
    26221 gggccaatcg ctgccggcct gggtcttcgc gcaggagtcc ttcatcgacg aggcggccca
    26281 tgccaccggc gccgacccca tcgaataccg tctcgagcac atcgacgacc cgcgcgcccg
    26341 cgcgctgatc gaggacgtga gccagcgcgc cggctggacg ccgcgcatca gcgacacgcc
    26401 cgatggcgcc ggccgcggct ttgcctacgc cagcgtgctc gatgccacgc aggacccgcc
    26461 gcggcaggtc tgggccgcct gggtggccga cgtcaaggtc gacccgcgga ccggcctgat
    26521 cgacgtcacc cgcctggtgg tgggccacga cagcgaagcc ggccgcaccg aactggacgc
    26581 cgccgcgctc gaccgcgaac tgctggccgc cacgcgccag ctgctgcagg cgcccgcccc
    26641 gcgcttcgac gactgggcca cgccccttgc cgacgacggc acggccggac ccgaatcaac
    26701 ccccgaatca accagcctca cgctgcgccc gctcatccag catgcgctgg ccgaaggctc
    26761 gcggcagctt gccgcgcccg gccagggcct ggcctggacg ccggccctcg ccctgcccgc
    26821 ggcggcggcc atcgccaatg ccgtcttcga cgccaccggc gtgcgcctgc gcgaaacgcc
    26881 gttcgacggc gccgccgtgc gccgccaatt aggcgccccg gccgcgcccg agcctcgcaa
    26941 gcgcccgctg gcccgcgcgg ccgcctggct gggcgccacg gcggccggcg tcgccggcgc
    27001 caccgccatg ctctggccct ggccggccgc catcgcgccc atcgtccagc ccgacatccc
    27061 gctgtattcc gccgccgcca tcgagcgcgg ccgcctggtg gcccaggccg gcgactgcgc
    27121 cgtctgccat accgcgccca gcggccgcga gaacgccggc ggcctggcgc tggacacgcc
    27181 cttcggcact atctacacca gcaacatcac gcccgacccg cagcatggca tcggcaactg
    27241 gtcgtacacc gccttcgagc gcgccatgcg cgaaggcata catcgcgacg gacggcgcct
    27301 gtacccggcc ttcccgtata ccgcctacgc caagctcagc gatgccgaca tgcaggccct
    27361 gtacgcctac ctgatgacgc gccccgccgt ggccgaaccc gccccggcca ccaagctggc
    27421 ctttccctac aacgcccggc ccctgctggc cggctggaat atgctgttcc acgacgacag
    27481 ccccttccag cccgatccga cccaatcgac gcagtggaac cgcggcgcct acctggtgct
    27541 gggcgccggc cactgcggcg cctgccatac gccgcgcaat gccctgggcg ccgagaaaaa
    27601 cggcaacgat ttcttgtccg gcggcctggc cgagggctgg caggcccccg cgctcaacac
    27661 gctgaaccag gccggcgtcc cgtggaccga gcaggacctc accgcctacc tgcgcaccgg
    27721 ctactcgccc ctgcacggcg tggccgccgg ccccatggcc cccgtcatcc acggcctggc
    27781 gcaactgccc gagcaggacg tcaaggccat ggccgtctac ctgctgtcct tgaaccaggg
    27841 cgcgcagccc gcggcccatg aagcagcccc gcagccggcc cccagccccg ccgcccaggc
    27901 cctcgccctg gaaagccgca gcgcgccgca gctgcgcttg ttcgacggct acgccaaggg
    27961 cgaacgcctg tacgagaacg cctgcgccgt ctgccacgaa gccggccagg gccccacgct
    28021 gcatggcgtc aagccttcgc tcgcgctcaa caccaatctg caggccggcc agcccgacaa
    28081 cctgatccag gtcatcctca acggcatcac gcagccggcc gaccccgcgc tgggctatat
    28141 gcctggtttc cgggacagtt tcgacgacca gcagatcaac gacctggtcg cttacctgcg
    28201 cgtgcgcttc ggcaacggcc agccggcctg gacgggactg caggagagcg tggcacgcat
    28261 acgagcgcag cccgtggcgc attgagcgtg cgcaatgaga tagtgtgagc gcaggcggtt
    28321 cgggc
//
LOCUS       DASWPO010000061        20947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3049767, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000061 DASWPO010000000
VERSION     DASWPO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20947
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3049767"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VGC69_03770"
     CDS             <1..690
                     /locus_tag="VGC69_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0294436.1"
                     /translation="DHSAGAHAALATLAALHQRRRTGLAQHVDLAAREVASAMIGDAL
                     VLASLGEVPARPGNADPGMAPYGVYPTSRADRWLTIAVRDDAQWRALARALGQEADDP
                     RFAAAADRLRRREELDVLLGQWLAGCDADATAQRLQQAGVSAHVSWSMQDIASDRHLR
                     ARRATVEVRAPDLEPRLAVGAPGRFSRTDDVGIRRLTPALGQDEDYVFGELLGLSSAQ
                     RADLEAREIIL"
     gene            730..1314
                     /locus_tag="VGC69_03775"
     CDS             730..1314
                     /locus_tag="VGC69_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY0294437.1"
                     /translation="MKNAIYLVLDMENDLVHADGISAKGPYGEQVRDRGILVHTRRAL
                     DKARAAGLHIGFVRVGFSPDYRECPPDSPIFSGARKNGIFKLGEWGTQVHPDLGRQPE
                     DFDIVKHRVSPFYSTSLQAILRANRIERIYCSGISTNAVVQAMVREGHDRDYTMVVLE
                     DCCCALSAEEHDGAIKSLQRFCCRVTNSRDVVFE"
     gene            1322..1549
                     /locus_tag="VGC69_03780"
     CDS             1322..1549
                     /locus_tag="VGC69_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294438.1"
                     /translation="MNRIARSLLRGPGGVDSVSVVNGAYQPAAVEVAWAERVIAAFEA
                     SRGAATEVDGKMIDKPMVARARRVMADHRQG"
     gene            1663..2292
                     /locus_tag="VGC69_03785"
     CDS             1663..2292
                     /locus_tag="VGC69_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0294439.1"
                     /translation="MDYRTKEEQVADYLRECIISGVYPRGSRLKQAEIAEQLRLSITP
                     VREALKLLAAEGYVSGDSYRGARVVPFDAAASGETLQLRLTLELQLVQGAIEKVTAQD
                     IIELRALADEFAQAFEKGDRPTARGINYRLHRRLYDIAQMPQTLHFVQILWARYPFDL
                     INSVSGRGEVAVHEHERILHAIVAGDVSAAMLAMRQHIESGWDVLKSGQ"
     gene            2361..3152
                     /locus_tag="VGC69_03790"
     CDS             2361..3152
                     /locus_tag="VGC69_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0294440.1"
                     /translation="MNDLADLPIPAHPMEVATASGTSRSEAVTLVLRDMITRGDFPAG
                     FHLQELPLAERMGVSRTPIREALATLAREGLLEPGPRRGYKVRTFSIEEIADAYEVRA
                     TLEGAACRLLAERGLPEDLIRRIRDLLDLGDQLLERGLFTRMEHEPWGEMNMALHQLL
                     VGAAGNALLEHQVQQTCNVPLAGPRDVHWYRLDQENFSLVQQAHRDHHAIFEAIVRRQ
                     SSRAEARMREHIYLSRDLVRERFRQQTVGFDAATITRTNQQDLTR"
     gene            complement(3118..4389)
                     /locus_tag="VGC69_03795"
     CDS             complement(3118..4389)
                     /locus_tag="VGC69_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0294441.1"
                     /translation="MTVVVVGSGQAGARAVEALRQNGYRGRVVLVGEEPYLPYDRPPL
                     SKDVLLKQGGQYSQEGDLVPVLDPDFYRARAIELRLGVRVVRIRRDDRAVDLADGSSL
                     GYERLILATGLRSRPLKGFELDDLLHHLRTLDDALRLRQALRPGLRLLVVGGGLLGLE
                     IAATARRIGCEVTVVERHPSLLYQSVAAVVGDHVTRLHHRHGVHILTGVTPCGMRVQT
                     TDPQSGARAIVTDLSNGETLVSDRVVAATGSLVNTELAEACGLDVQDGIIVDAYGRTS
                     DPSIYAVGDVARHYNPVLGRTIRVESWHNAQHQSAAVARVIAGAKTPYAEVPWFWSDQ
                     FDMNLQTVGYCSDWDRVVVRGDPNGRRFSALYLQGNRLVAANLVNNGREMPQAQRLIA
                     SQAALDVASLAQVERLDQAVISASNPADSSV"
     gene            4528..4911
                     /locus_tag="VGC69_03800"
     CDS             4528..4911
                     /locus_tag="VGC69_03800"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY0294442.1"
                     /translation="MHTYREFHWETRKLGEAAKKTKWIPIAKVGDTISAYGMPVEIEG
                     EEIALFMIDGCRYATSNICTHQLAYMTDGYVDGETVVCPMHQGRFHIPTGAPQGAPVS
                     KPLRVYPVRVEGDDVLIEFSPSPPA"
     gene            4999..6183
                     /locus_tag="VGC69_03805"
     CDS             4999..6183
                     /locus_tag="VGC69_03805"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0294443.1"
                     /translation="MNPQKVTEIVNRIEQSGRAVRQPLARASHVPYQLYSDPDVLELE
                     KERLFMKDWIVVGRVEQIAKPGDYMTLDIFNEPIVVTRDNEGQINAFSNFCLHRGAEI
                     SAKGFGNTQEFSCPYHNWLYDLKGHLMGAPHMHHTEGFDTQKCQLPRVRVDTWGGFIF
                     VCFDADTPPLAQFIGRFAEEFAFLRMEDCAMGARVEFNLNCNWKFVVENLFDVYHVTT
                     IHAGTFGKYRDTVDYFKDAPGQDSLFGYYKSAPSMDGGKSLFGHMPWLADKPDTFACS
                     GFQGPNLQMFARCDGLIVHSIWPVTADTSRMISYMLFPKEFFADPEFETKAARYAGYL
                     RKIIDEDEMAITSLQRAAHSRLFQPGRMSRLEHGVYNVLNYYLDRIVPNDAARAAGEL
                     AR"
     gene            6222..7445
                     /locus_tag="VGC69_03810"
     CDS             6222..7445
                     /locus_tag="VGC69_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294444.1"
                     /translation="MDPSRRNLIRQGGSLMLAASLSSVMARALAQSPGEPLRVGALND
                     VTGAGSPYGAGMQKAILMAADEVNAAGGVAGRKIQVYAEDSQTSPEAGVLAAKKLIEV
                     NKVQAILGTWSSGVAAAVMPITDTAGVLHATNAGASNILSSKGLMFRYSALSVRVGVA
                     EAATLAQKGYQRLATMAFNNPSGREITAGVKQGWEKLGRKLVAEVVYEPNQPTYRSEI
                     QRVLAGNPDAIALGGYLPDFTVILRETRQMGSDVKFYVPAWAVNQKLIDALGPAATSG
                     VFTYDYVAALDGEAFKTFVEKFKKATGVDPADNYYACCAYDMMVTFALAAEAAADKSP
                     VADAMRAVANPPGTPVYDVTQGLKLLRSGQKINYEGASGPIDFDDQGNVKPLFKMSEI
                     RNGKVEDIGMVFQDY"
     gene            7458..8333
                     /locus_tag="VGC69_03815"
     CDS             7458..8333
                     /locus_tag="VGC69_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0294445.1"
                     /translation="MTGWIFYANLVVNGVVEGLLIGLAALALNMVFAVGRFPNAATGD
                     FMTVGAYAGFGAHYLGGGIAAQALAAMAACVALSLLCYLAVFRRLAGRPMVAAMLASI
                     GLGFFCRSMLSLFVGSDPQFFPMGAATSVQLGGLRIATSDLWLAVLALACVGAVMALL
                     HLTPIGRRIRAVADNAPLAQASGIRSRRVMLTLWAMVGAICGLAGLILGIRTTVNPEM
                     GWGLLLPMFAAAILGGVGSPLGAVLAGVLLGVLQELSTLWVGFSYKIAVSFAILALML
                     LVRPQGMFGLREAVR"
     gene            8333..9223
                     /locus_tag="VGC69_03820"
     CDS             8333..9223
                     /locus_tag="VGC69_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0294446.1"
                     /translation="MLAYLVALLIVMGIYALLTISLDLQYGFTGLVNFGLVGFFSIGA
                     YASALVTLAGHPMPLGFAAAMLASGLAALPIGALALRLRDDYFAIVSLGFSEIVRLVV
                     LNEEWLTKGGLGLTGVPRPFTRFGVGGSELAYLALVAACLAAVVLVVRRVTGSPFGRL
                     IQAIRDNEVAVISLGKSPPAAKVKALVLGAAIAGLAGALYAHYITYVVPDQFLPLVTF
                     YVWMAMILGGLGSLRGALAGAGVLVLILEGTRFVRDLVPGVGAVQMASVRLALVGLLL
                     VVFVLFRPQGAFGNPARTWR"
     gene            9220..9972
                     /locus_tag="VGC69_03825"
     CDS             9220..9972
                     /locus_tag="VGC69_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294447.1"
                     /translation="MNLVLTRIGKAWGGLRVLDEVSMTVDGGAITGLIGPNGAGKSTL
                     FAAISGNVPVDSGSISLDGLSLDSLGPGRRARLGLMRTFQVPRPFANLTVRQNLAVAV
                     RAQPGESLLNVFFRPGAVRRVEQEIAARAEEVIGSLNLARVADRMASQLSGGQLKLLE
                     LGRLLMTRPRLILLDEPFAGVNPVLAEEIADRIRALHAQGLGFFIVEHNLGALSKLVA
                     TLFAMDRGTLIASGAPDEVLANDQVRTAYVGG"
     gene            9959..10696
                     /locus_tag="VGC69_03830"
     CDS             9959..10696
                     /locus_tag="VGC69_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294448.1"
                     /translation="MWGAEVKLSPDDRTLLELRGLQGGYGEADILRGVDLVVDAGEIV
                     TIAGTNGAGKSTLAKAVMGLLPRCAGVRRFRGVDLVRRAPEDRVLMGISYVPQVANVF
                     ARLTVQENLEVVEGVKDRRARIEEMYALFPGLHERRRLHAGLLSGGERQQLAFARALM
                     PRPVMLLLDEPTAALSPALAAQVFDTIARLPALGVAALVVEQRARQSLQISDRGYILD
                     QGRVVMEGRAQDLLSSAEMTDLYLGQH"
     gene            10726..11778
                     /locus_tag="VGC69_03835"
     CDS             10726..11778
                     /locus_tag="VGC69_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294449.1"
                     /translation="MHTLSPFDPPAQRAANLLIADHMGVKPGETVLITTDLRGDPQVA
                     LMLMNAAAQIGARPMVVTLQQLPLQGKLADPHLPDALRVAMAASDCWIDLTHPYIAGS
                     ETHDHAVKAGRVRCLVAGGMDSGALARLYAGVPLDTLYELQQMMDQLIADSMGKECRL
                     TDANGTDVRFVLGKATGSKPRRALAAGATYSLPGSVVMYPDLPSVQGRIVIVAAMHDW
                     YGPLERPLTLEVDGRIQRIVEDGPDTLLLDRALRRAGGGDYGHVIHFSYGLHPAARWR
                     GNCFVEDIRTVGANAIGFGRPWWEPGGGENHPDGLLVRQNLWIDGRQVLQSGMVVAPS
                     SMAAVARRVADAASPG"
     gene            11825..12676
                     /locus_tag="VGC69_03840"
     CDS             11825..12676
                     /locus_tag="VGC69_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0294450.1"
                     /translation="MICYPFGVGPVVTRIFEAGASDAPVILLLHGLSSRADRWIRNID
                     ALAAAGYRVIAPDLPGHGFATKDPAQDHSIPGYGSFVLGLLDAMGIPKATIVGTSLGG
                     HIVANAVLRQPERAERLMMIGSTGLAPTAPARAQGMRDWLMNLTPDSHRPKLRNVFTD
                     KSLASDDMVVEDVRINTSPGASACFDAFLAYMADGINDDLVLERLPEIAERVPLLLFW
                     GVDDSSVSVDVGRAAHARLPKSRLITVEHLNHTPYYENPPLFNEVLLKFIAGRIDDVQ
                     APGLTLS"
     gene            complement(12722..13336)
                     /locus_tag="VGC69_03845"
     CDS             complement(12722..13336)
                     /locus_tag="VGC69_03845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0294451.1"
                     /translation="MENDVLTRLVDQLLNDPRYAGHPLREALKGLQEQNRVLKIASTH
                     DPLTGIGNRALLTRCLQEESQRVQRTGRSYCLAILDVDNFKRINDTWGHAAGDLVLKA
                     IAQALMSSIREGDICGRWGGEEFLVIMPDVGLAEARHVVEHFNQTVAELAIPAEAAII
                     RITSSVGATQYTPNEHYAQALQRADGALLQAKQAGRARCIYQYP"
     gene            13489..13842
                     /locus_tag="VGC69_03850"
     CDS             13489..13842
                     /locus_tag="VGC69_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0294452.1"
                     /translation="MASTDLTNLTKSVGQAIAKRRTGCGLTQEQVAERLGIGVEAVSR
                     IERGVVLPTVARLGEFADIFECNIADLVTETSSRVTDQASHLAGLLSRLGSADRALVL
                     DIVETLIARLGRPRH"
     gene            complement(13839..14156)
                     /locus_tag="VGC69_03855"
     CDS             complement(13839..14156)
                     /locus_tag="VGC69_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004531864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294453.1"
                     /translation="MAHTAAAPASSGPRLTLGKVSITDSASAALAKAGAEGVFLLARH
                     LYGDWGDCTAKDALQNELAVLLGLRVLSRYALSGTNAIWIITEADRAATTIMAPDLGQ
                     AQG"
     gene            complement(14235..15500)
                     /locus_tag="VGC69_03860"
     CDS             complement(14235..15500)
                     /locus_tag="VGC69_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294454.1"
                     /translation="MNQQSLTEPSAPQAEATTWVMRGTPAYRRIGFALFLAGFSTFSL
                     LYCVQPLLPMFTEEFAIGPAASSLALSLSTGLLAISILCAGALSQRVGRRGLMFVSMT
                     LAAVFNLLAACAPTWHGLLVMRALGGLALGGVPAVAMAYLAEEIHPASLGLTMGLYVG
                     GTAFGGMIGRVGMGMLGEAMSWRAALMAISIVDLAAALAFVLMLPASRHFVASRNLGF
                     SHHFTLWRRHLSNSALRKLFAVGCLVMGVFVTIYNYAGFRLAAAPFSLTPTQTSLIFC
                     AYLFGIVASSLAGALADRFGRPPVLRIGILVTAAGLALTLAHTLLPVVAGIVLLTIGF
                     FITHSVASGWVGGIAEGAKGHASALYLLAYYLGSSVLGSIGGGFWQMGGWNAVGGYAF
                     VMLALCIGLALSLRGARAACGRPWMKTST"
     gene            15610..16491
                     /locus_tag="VGC69_03865"
     CDS             15610..16491
                     /locus_tag="VGC69_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294455.1"
                     /translation="MELRHLRYFLAIAAEANFTRAAQKLGIGQPPLSQQIKGLERELG
                     VELFRRTAHGVVLTPAGEAFRDEAARVVEGAQDAVRAAQRAARGETGRMRLGFTGSAA
                     FNPVVSELIRQFNRDYPKADLVLVEDNTMQLLQALADNRLDAIFFRPELGGSEGLRLH
                     RFQDEPMKIVLPARHPLARHRALPLSALAGEAFVIVPGSAGATLYNEIMNLCRELGFE
                     PALAQPAPQVSSVINLVAAGLGISIVPAALAQVRVAGVRYLDIRPPAPVARLALAARA
                     DNASVVVRNCFELAAGI"
     gene            complement(16515..17291)
                     /locus_tag="VGC69_03870"
     CDS             complement(16515..17291)
                     /locus_tag="VGC69_03870"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294456.1"
                     /translation="MVEAVQAAGAAVSLRRVDHAFDLGGSSLPVLSNIDLEVRPGEFL
                     ALLGPSGCGKSTLLRLVAGLETPSSGSIAADGEEILRPDPSRILVFQDPTLYPWRTVR
                     DNVALGLQARGLLPARNARIDDALERVKLAEFAGAYPHQLSGGMAQRAALARALVNDP
                     RILILDEPLGKLDSLTRLAMQSELLELWRRTGITVLLVTHDVEEALFLADRIIVLSDR
                     PARIKDEIINDLPYPRHRGDPRLVAMRQRALRQLGLDASW"
     gene            complement(17285..18334)
                     /locus_tag="VGC69_03875"
     CDS             complement(17285..18334)
                     /locus_tag="VGC69_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0294457.1"
                     /translation="MPPHTELAVNAHDARPSPGSPRLSGTALARFWPSGLLAAVLWAA
                     TGLVTLLWPDLQVGFKEWRYTDAFGTAALAIAVLLAALAVSGLASRSPNRLLRGLRLA
                     GPWLALLAVAIGLWEIATAKLALLPSPFFAPPQALIEVYAQDWPRLLDSLWHSLWLLA
                     NGYALGAFFGFVTGVAIGWSRRLGYWVHPVLRFLGPVPSTALLPISFFVFPSSFSAAV
                     FLIALSTWFPVTVLTWSGVASVNRAYYDVARTLGASQAFLIFRVAIPAALPHVFVGLF
                     MGLGHAFSVLVVAEMMGVKSGLGWYLSWAQGWASYANMYGALIVMAVTFSGIITLLFV
                     VRDRLLAWQKGVVKW"
     gene            complement(18337..19341)
                     /locus_tag="VGC69_03880"
     CDS             complement(18337..19341)
                     /locus_tag="VGC69_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294458.1"
                     /translation="MNQSAAWAARRRFLQGSLGATAAIMAGKTGLALAQPAEPLKKVS
                     IAWSATAVCTVPVPAALEQGYFRKYGLDVSFINFAGSTDQLLEAIASGKAQAGVGMAL
                     RWLKPLEQGFDVKIISGVMSGCIRLLVAKDSPYASVADLKGKTIGVSDMASPAKNFFS
                     ILLGQAGVDPEKDVAWRAYPADLLGQGLQKGEVHAVADNDPIISLVRKNFGLRELASN
                     MTGKYADRSCCVLAVSGNMVRNDFKTAQALAAAIQEGSEWTAENPRKAAELFASYSPK
                     VPVDDLVEMLAGMSHHHHPVHQPLKQEIADYVEELKAIHVISPGIDPQKFAAAVTADV
                     "
     gene            19550..20185
                     /locus_tag="VGC69_03885"
     CDS             19550..20185
                     /locus_tag="VGC69_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HEY0294459.1"
                     /translation="MTTLSSQPARADAAATAPLREFIASLSGLLDRQPAEAQILREGG
                     ALLARLVARDGWLPDEYAVPNPDRYQQFLLHMDSAQRFCVVSFVWGPGQSTPIHDHTV
                     WGLIGMLRGAEYSQPYRLHEDGGAREHGAAIRLEPGQVEAVSPRVGDIHKVSNAYADR
                     ASISIHVYGANIGAVRRSVYAVDGARKPFISGYSNTSLPNIWDLSTMSKPS"
     gene            20182..>20947
                     /locus_tag="VGC69_03890"
     CDS             20182..>20947
                     /locus_tag="VGC69_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0294460.1"
                     /translation="MSSPDASFPSTSYAQVRRALIDGTEIALMDVREEDPFAQEHPLW
                     AANFPLSRLEIDTWERIPRRDTRIVVYGHDESGDLAPRAAAVLRAQGYADVSLLDGGL
                     EGWRAAGGELFRDVNVPSKSFGELVEATRHTPSLSAQEVGQLIEDGAGTLVVDVRRYD
                     EYHTMSIPSATSVPGAELVLRIRELAPDPSTRVIVNCAGRTRSIIGAQSLVNAGIPNP
                     VAALRNGTIGWTLAGLELAQGATARAPEVGAEVVALA"
BASE COUNT         3455 a         6914 c         7146 g         3432 t
ORIGIN      
        1 gatcattccg ccggcgcgca tgccgcgctg gccacgctgg cggccttgca tcagcgcagg
       61 cgaacgggct tggcccagca cgtggatctg gccgcgcgcg aagtcgcgtc ggccatgatc
      121 ggcgacgcgc tggtcctggc cagcctggga gaggtgccgg cgcggccggg caacgccgat
      181 cccggcatgg cgccgtacgg cgtgtatccg acctcgcgcg ccgaccggtg gctgacgatc
      241 gccgtgcggg acgacgccca atggcgggcg ctggcgcggg cgctgggcca ggaggccgac
      301 gatccgcgct tcgccgcggc ggcggaccgg ctgcgccggc gcgaagagct ggatgtcctg
      361 ctcgggcaat ggctggccgg ctgcgatgcc gacgccaccg cgcagcgctt gcagcaggcg
      421 ggcgtgagcg cgcatgtatc ctggtccatg caggacatcg cgtcggatcg gcatctgcgg
      481 gcgcgccgcg ccacggtcga ggtccgcgcg ccggacctcg aaccgcgcct ggccgtgggc
      541 gcgccgggcc ggttcagccg cacggatgac gtgggtatcc ggcgcctgac tcccgccctg
      601 gggcaggacg aggactatgt cttcggcgaa ctgctgggct tgagctcggc gcagcgcgcg
      661 gatctcgaag cgcgcgaaat cattctgtga cgccggcgcg cctgctggcc atcacgcaaa
      721 ggacaatcga tgaaaaacgc catctatctg gtgctcgata tggaaaacga cctggtccac
      781 gccgacggga tcagcgccaa ggggccctac ggcgaacagg tgcgcgaccg gggcatcctg
      841 gtccacaccc ggcgggccct cgacaaggcg cgcgcggcgg gcttgcacat cggcttcgtg
      901 cgcgtggggt tttcgccgga ctaccgtgaa tgtccgcccg attcgccgat tttttccggc
      961 gcgcgcaaga acggcatctt caagctgggc gaatggggca cgcaggtgca tcccgacctg
     1021 ggccgtcagc ccgaggattt cgacatcgtc aagcatcgcg tgagcccttt ctacagcacc
     1081 agcctgcaag ccatcttgcg cgccaaccgc atcgagcgca tttattgctc gggcatttcc
     1141 accaatgccg tggtgcaggc catggtgcgc gagggacacg accgcgatta cacgatggtg
     1201 gtgctggagg attgctgctg cgcgctgtcg gccgaagagc acgacggcgc gatcaagagc
     1261 ctgcagcgat tctgctgccg cgtcacgaac tcgcgggacg tcgtgttcga gtgaggcgac
     1321 gatgaaccgc atcgcgcgca gcctgctccg cggacctggc ggcgtcgatt cggtttcggt
     1381 ggtgaacggc gcctatcagc ctgccgcggt cgaggtggcc tgggccgagc gggtcatcgc
     1441 ggccttcgag gccagccggg gtgctgccac cgaagtggac ggcaagatga tcgacaagcc
     1501 gatggtggca agggcccggc gcgtcatggc ggaccatcgc caggggtgag gggcgccgca
     1561 ggcggcgtgc cggctatccg ctacgtataa aatgcattcc atctcggggc gccatggcgc
     1621 ccgtcgaggt ttccgagaac aaggtcgacc cgggcgccgc agatggatta tcgaacaaag
     1681 gaagagcagg tcgccgacta cctgcgcgaa tgcatcatat cgggtgtata tccccgtggg
     1741 tcgcggctaa aacaagcgga aatcgcggaa cagctgcgct tgagcattac tcccgtgcgc
     1801 gaagcgctca agctgctggc cgccgaaggc tacgtgagcg gggactccta ccgcggcgcc
     1861 cgcgtcgtgc ccttcgacgc ggcggcctcg ggcgagacgc tgcaattgcg cctgacgttg
     1921 gagttgcaat tggtccaggg cgcgatcgaa aaggtcaccg cgcaggacat catcgagctg
     1981 cgcgcgctgg ccgacgagtt cgcccaagcc ttcgagaagg gcgaccgtcc cacggcgcgc
     2041 ggcatcaact accgcctgca ccgccgcctc tacgatatcg cgcagatgcc gcagaccctg
     2101 catttcgtgc agatactctg ggcgcgctat cccttcgacc tgatcaattc cgtgtcgggc
     2161 cgcggtgagg tcgccgtgca cgagcacgag cggattctgc atgccatcgt cgccggcgat
     2221 gtctccgcgg ccatgctggc catgcgccag cacatcgaat cgggatggga cgtgctcaag
     2281 tccgggcagt aggcaataaa atcaaggact ggccgacttt tcgagctttc gcggcattca
     2341 tccttcgcaa ccgggcccac atgaacgacc tcgccgattt gcccataccc gcacacccga
     2401 tggaagtcgc gacggcttcg ggtacttcgc gctcggaggc ggtcacgctg gtgctgcgcg
     2461 acatgatcac gcgcggcgat tttcccgcgg gctttcactt gcaggaactg ccgctggccg
     2521 agcgcatggg cgtgtcgcgc acgccgatcc gcgaagcgct ggccaccctg gcccgcgaag
     2581 ggctgctcga gcccgggccc cggcgaggtt acaaggtgcg cacgttctcg atagaagaaa
     2641 tcgccgatgc ctacgaggtg cgcgcgacgc tggaaggcgc ggcctgccgg ctgctggccg
     2701 agcgcggcct gccggaggac ttgatccgcc ggatccgcga cctcctggac cttggcgacc
     2761 aattgctcga gcgcggcctt ttcacccgga tggagcacga gccctggggc gagatgaaca
     2821 tggccctgca ccaattattg gtcggggccg ccggcaatgc gctgctcgag catcaggtgc
     2881 agcagacctg caatgtgccg ctggccggtc cgcgcgacgt gcattggtac cggctggacc
     2941 aggagaattt ctcgctggtg cagcaggccc atcgcgacca tcacgcgatt ttcgaagcca
     3001 tcgtgcggcg ccagtcgagc cgcgccgagg cgcgcatgcg cgagcacatc tacctgtcgc
     3061 gcgacctggt gcgcgagcgc ttccggcagc agacggtcgg attcgacgcg gcgacgatca
     3121 cacggacgaa tcagcaggat ttgacgcgct gatcacggcc tgatccagcc tctccacctg
     3181 cgccaggctg gccacgtcca gggcggcctg cgaggcgatc aggcgctgcg cctgcggcat
     3241 ttcccgcccg ttgttcacca ggttggccgc gaccagccgg ttgccttgca ggtacagggc
     3301 ggagaagcgg cggccgttcg gatcgccgcg cacgacgacg cggtcccagt ccgagcagta
     3361 gcccacggtt tgcaggttca tgtcgaactg gtcggaccag aaccagggca cttcggcgta
     3421 aggcgttttc gcgcccgcga tgacgcgcgc cacggcggcc gactggtgct gggcgttgtg
     3481 ccaggattcg acccggatgg tgcggcccag caccgggttg taatggcgcg cgacatcgcc
     3541 gacggcgtag atggacggat cggacgtgcg tccgtaggca tcgacgatga tgccgtcctg
     3601 cacgtccagg ccgcaggcct cggccagttc ggtgttcacc agcgagccgg tggcggccac
     3661 cacgcggtcc gataccagcg tctcgccatt ggaaagatcg gtgacgatgg cgcgcgcgcc
     3721 gctctgcgga tcggtggtct gcacgcgcat gccgcacggc gtcacgcccg tcaggatgtg
     3781 cacgccgtgg cggtggtgca gccgcgtcac gtggtcgccg acgaccgcgg cgaccgactg
     3841 gtagagcagc gacgggtggc gctccaccac cgtgacctcg cagccgatcc ggcgcgcggt
     3901 cgccgcgatc tccaggccca gcaggccgcc gcccaccacc agcaggcgca gcccgggcct
     3961 gagcgcctgg cgcagccgca gcgcgtcgtc cagcgtgcgc aggtgatgaa gcaggtcgtc
     4021 cagctcgaac cccttgagcg gccgcgaacg caggccggtt gccaggatca gccgctcgta
     4081 gccgagcgac gagccgtcgg ccaggtccac ggcgcggtcg tcgcgccgga tccgcaccac
     4141 gcgcacgccc aggcgcagct cgatggcgcg agcccgatag aagtccggat ccagcacggg
     4201 taccagatcc ccttcctgcg agtactgccc gccttgcttg agcagcacgt ccttcgacag
     4261 cggcggccgg tcgtagggca ggtaaggctc ttctcccacc agcacgacac ggccccggta
     4321 gccattctgg cgcaaggctt ccacggcgcg ggcgcccgcc tggcccgagc cgacgacgac
     4381 aacagtcatt ctcacctcat gtatacaaaa aataatgcac cgctttacgg caacctgcgc
     4441 aagcttggtg catttcatgg atatgtatgc accaatgcgg atttttcaca aaaaatactt
     4501 tactcaccat agggtgagtc gtattttatg catacttatc gagaatttca ctgggagacc
     4561 agaaaattgg gcgaggcagc aaaaaagacc aaatggatac caatcgccaa agttggcgac
     4621 accatctccg cgtatggaat gccggttgaa atcgagggcg aagagatcgc gctgttcatg
     4681 atcgacgggt gccgctatgc cacgagcaat atctgcacgc accagctcgc gtacatgacc
     4741 gatggttatg tcgacggcga gaccgtggtg tgccccatgc accaggggcg ctttcacatc
     4801 ccgaccggcg cgccccaggg agcgccggtt tccaagcccc tgcgcgtcta tccggtgcgt
     4861 gtggagggcg acgatgtgct gatcgagttt tcgcctagcc cgccagcctg attccagccc
     4921 tgattgtcag gccctgattc tccaccgttt ccacccgatt ctccctgcca tccaccttga
     4981 aaagaggggc gccccgtcat gaatccgcag aaagtcaccg aaatcgtcaa ccgcatcgaa
     5041 cagagcggac gcgccgtccg ccagccgctg gcccgggcca gccacgtgcc ctaccagctc
     5101 tacagcgacc ccgacgtgct ggagctggaa aaggaacggc tgttcatgaa ggactggatc
     5161 gtcgtgggcc gcgtcgagca gatcgccaag ccgggcgact acatgacgct ggacattttc
     5221 aatgaaccca tcgtcgtgac ccgcgacaac gaaggccaga tcaacgcctt ctcgaatttc
     5281 tgcctgcacc gcggcgcgga gatctcggcc aagggcttcg gcaataccca ggagttcagc
     5341 tgcccctatc acaattggct gtacgacctg aagggccatc tgatgggcgc cccgcacatg
     5401 caccacaccg agggcttcga cacgcagaag tgccagctgc cgcgcgtgcg cgtggatacc
     5461 tggggcggct tcattttcgt ttgcttcgat gcggatacgc cgccgctggc gcagttcatc
     5521 gggcgtttcg ccgaagagtt cgccttcctg cgcatggaag actgcgccat gggcgcgcgc
     5581 gtggaattca acctgaactg caactggaag ttcgtcgtcg agaacctgtt cgacgtctat
     5641 cacgtgacca ccatccatgc cggcactttc ggcaaatacc gcgacacggt ggactatttc
     5701 aaggacgcgc cgggccagga ctcgctgttc ggctactaca aatccgcgcc gtccatggat
     5761 ggcggcaaga gcctgttcgg ccacatgccc tggctggccg acaagcccga taccttcgcc
     5821 tgcagcggtt ttcagggccc caacctgcag atgttcgccc gctgcgacgg cctcatcgtg
     5881 cattcgatct ggccggtcac ggccgatacc agccgcatga tcagctacat gctgttcccc
     5941 aaggaattct tcgccgatcc ggagttcgag accaaggccg cgcgctacgc cggctacctg
     6001 cgcaagatca tcgacgagga cgagatggcc attacctcgt tgcagcgcgc cgcgcattcg
     6061 cgcctgttcc agcccggccg catgtccagg ctcgagcacg gcgtctacaa cgtcctcaac
     6121 tactacctgg atcgcatcgt gcccaacgat gccgcccggg ccgccggcga actcgcgcgc
     6181 tgaaggcgcc tttttcctcc cctttttttc cggagacttc catggaccca tcgcgtcgca
     6241 atctgatccg gcagggcggc agcctgatgc tggccgcctc gctgtcttcc gtcatggccc
     6301 gtgcgctcgc tcaatcgccg ggcgaaccgt tgcgcgtcgg cgcgctcaac gacgtcaccg
     6361 gcgccggcag tccttacggc gccggcatgc agaaggccat actgatggcc gcggacgagg
     6421 tcaacgcggc cggcggcgtg gcgggacgca agatccaggt ctatgccgag gattcgcaga
     6481 cttcgcccga ggccggcgtg ctcgcggcca agaagttgat cgaggtgaac aaggtgcagg
     6541 ccatcctggg cacctggtcg tccggcgtcg cggccgcggt gatgcccatt accgacacgg
     6601 cgggcgtcct gcacgccacc aacgcgggcg cctccaacat cctcagcagc aaagggctga
     6661 tgttccgtta ttcggcgctg agcgtgcggg tgggggtggc cgaggccgcc acgcttgccc
     6721 agaaggggta ccagcggctg gccaccatgg cgttcaacaa cccgtccggc cgggaaatca
     6781 ccgcgggcgt caagcagggc tgggaaaagc tcggccgcaa gctcgtggcc gaggtggtgt
     6841 acgagcccaa ccagccgacc taccgatccg agatccagcg cgtgctggcc ggcaacccgg
     6901 acgccatcgc gctgggcggc tacctgcccg acttcaccgt catcctgcgc gagacgcgcc
     6961 agatgggctc cgacgtgaag ttctacgtcc ctgcctgggc cgtgaaccag aagctcatcg
     7021 acgcgctcgg gccggccgcg accagcggcg tgttcaccta cgactacgtg gccgcgctcg
     7081 acggcgaggc gttcaagact ttcgtcgaga agttcaagaa ggccaccggc gtcgacccgg
     7141 ccgacaacta ttacgcctgt tgcgcctacg acatgatggt cacctttgcg ctggcggccg
     7201 aggcggccgc cgacaagtcg ccggtggcgg acgccatgcg cgccgtggcc aacccgccgg
     7261 gcacgccggt ctacgacgtg acgcaggggc tcaagctgct gcgctcgggc cagaagatca
     7321 actacgaggg cgcgtccgga ccgatcgatt tcgatgacca gggcaacgtc aagccgctgt
     7381 tcaagatgtc cgaaatccgc aatggcaagg tagaggacat cggcatggtg ttccaggatt
     7441 attgaacggc gcatgcgatg actggctgga tcttctatgc gaacctggtc gtcaacggcg
     7501 tcgtcgaggg gctgctgata ggcctggcgg ccttggcatt gaacatggtg ttcgccgtcg
     7561 gccgcttccc gaacgcggcc accggcgact tcatgacggt gggcgcctac gcgggcttcg
     7621 gcgcgcacta cctgggcggc ggcatcgcgg cccaggcgct ggcggcgatg gcggcctgcg
     7681 tggcgttgtc gctgctgtgc tatctggccg tgttccgccg cctggccggc cggcccatgg
     7741 tcgcggccat gctggcttcg atcggcctgg gcttcttctg ccgcagcatg ctgtcgctgt
     7801 tcgtgggcag cgatccgcag ttcttcccga tgggcgcggc gacgtccgtg cagctcggcg
     7861 ggctgcgcat cgccacctcc gacctgtggc tggcggtgct cgcgctggcg tgcgtgggcg
     7921 cggtcatggc gctgctgcac ctcaccccca tcggccggcg catccgcgcc gtggccgaca
     7981 acgcgccgct ggcgcaggcc tcgggcatcc gctcccggcg cgtgatgctg acgctgtggg
     8041 cgatggtggg cgcgatctgc ggcctggccg ggctgatcct gggcatccgc accacggtca
     8101 accccgagat gggctggggc ctattgctgc ccatgttcgc ggccgccatc ctgggcggcg
     8161 tcggcagccc gctgggcgcg gtgctggccg gcgtgctgct gggcgtcctg caagagctgt
     8221 ccacgctctg ggtgggcttt tcctacaaga tcgcggtgtc cttcgcgatc ctggcgctga
     8281 tgctgctggt ccggccccag ggcatgttcg gcctgcggga ggcggtgcgc tgatgctggc
     8341 ctacctggtt gcgctgctga tcgtcatggg catctatgcc ctgctcacca tcagcctgga
     8401 tctgcagtat ggattcaccg gcctggtgaa cttcggcctg gtcggcttct tctcgatcgg
     8461 cgcctatgcc tccgcgctgg tgacgctggc cggccacccc atgccgctcg gtttcgcggc
     8521 ggcgatgctg gccagcgggc tggcggccct gcccatcggc gccctggcgc tgcgcttgcg
     8581 cgacgactac ttcgccatcg tctcgctcgg cttctccgag atcgtgcgcc tggtggtcct
     8641 gaacgaagaa tggctcacca agggcggcct ggggctgacc ggcgtgccgc ggccgttcac
     8701 ccgtttcggc gtgggcggct cggagctggc ctacctggcg ctggtggcgg cctgcctggc
     8761 ggcggtcgtg ctggtcgtgc ggcgcgtgac cggcagcccg ttcgggcggc tgatccaggc
     8821 catccgcgac aacgaagtgg ccgtgatctc gctgggcaag tcgccgccgg ccgcgaaggt
     8881 caaggcgctg gtgctgggcg cggccatcgc cggcctggcg ggcgcgctct acgcccacta
     8941 catcacctac gtagtgcccg accagttcct gcccctggtg acgttctacg tctggatggc
     9001 gatgatcctc ggcggcctgg gcagcctgcg cggcgcgctc gcgggcgccg gggtgctggt
     9061 gctgattctg gaaggcacgc gcttcgtgcg cgatctcgtg ccgggggtgg gcgcggtgca
     9121 gatggccagc gtgcgcctgg ccctggtcgg cctgctgctg gtggtgttcg tgctgttccg
     9181 gccccagggc gctttcggca accccgcgag gacctggcga tgaatctggt gcttacgcgc
     9241 atcggcaaag cctggggcgg cctgcgggtg ctggacgagg tcagcatgac cgtggacggc
     9301 ggcgcgatca cgggcctcat cgggcccaac ggcgcgggca agagcacgct gttcgccgcc
     9361 atcagcggca acgtgccggt cgacagcggc tccatctcgc tcgacggact atcgctggac
     9421 agcctgggcc ccggccgtcg ggcgcgcctc ggcctgatgc ggacgttcca ggtgccgcgg
     9481 cccttcgcca acctgacggt gcgccagaac ctggccgtgg ccgtgcgcgc gcaacccggc
     9541 gagtcgctgt tgaatgtgtt cttccggccc ggcgccgtgc gccgtgtcga gcaggaaatc
     9601 gccgcgcgcg ccgaggaggt gatcgggtcg ctgaacctcg cgcgcgtggc cgaccgcatg
     9661 gccagccaat tgtcgggcgg gcagctcaag ctgctggagc tcggccgctt gctgatgacc
     9721 cggcccaggc tcattttgct cgacgagccc tttgccggcg tcaatccggt gctggcggag
     9781 gaaatcgccg accgcatccg ggccttgcat gcccagggcc tggggttttt catcgtggag
     9841 cacaacctcg gcgcgctctc gaaactggtc gcgacgctgt tcgccatgga tcgcggcacg
     9901 ctgatcgcca gcggcgcgcc ggacgaggtg ctggccaacg accaggtgcg gaccgcctat
     9961 gtggggggct gaggtgaagc tatcgccgga cgaccgcacg ctgctggaac tgcgcggcct
    10021 gcagggcggc tacggcgaag cggatatcct gcgcggcgtg gatcttgtcg tcgacgccgg
    10081 cgagatcgtc accatcgcgg gcaccaacgg cgcgggcaag agcacgctgg ccaaggccgt
    10141 gatgggcctg ctgccgcgct gcgcgggcgt gcgccggttt cgcggcgtcg acctggtccg
    10201 ccgcgcgccg gaagatcgcg tgctgatggg aatcagctac gtgccgcagg tcgccaatgt
    10261 cttcgcgcgc ctgacggtgc aggaaaacct ggaggtcgtc gaaggcgtga aggaccggcg
    10321 cgcgcgcatc gaggaaatgt atgcgctgtt tcccggcctg cacgagcgcc ggcgcctgca
    10381 cgccggcctg ctctcgggcg gcgagcggca gcagcttgcc ttcgcccgcg cgctgatgcc
    10441 gcggccggtc atgctgttgc tggacgaacc caccgcggcc ctgtcgccgg cgctggccgc
    10501 gcaagtgttc gacaccatcg cgcggctgcc cgcgctgggc gtggcggcgc tggtggtcga
    10561 acagcgcgcg cgccagagcc tgcagatcag cgaccgcggc tacatcctcg accagggccg
    10621 cgtcgtgatg gaaggccgcg cacaggacct cctgtccagc gccgagatga ccgacctgta
    10681 cctgggccag cactgacggc ccccactgtt cacggaaccc ccgccatgca cacgctctct
    10741 cctttcgatc cgccggcgca gcgcgccgcc aacctcctga tcgccgatca catgggcgtc
    10801 aagccgggcg agacggtgct aatcaccacg gacctgcgcg gggacccgca ggtcgctctc
    10861 atgctcatga acgccgccgc ccagatcggc gcgcggccga tggtcgtgac gctgcagcag
    10921 ctgcccttgc agggcaagct ggccgatccg cacctgcccg atgcgctgcg cgtggcgatg
    10981 gccgcctcgg attgctggat agacctgacc catccgtaca tcgccggttc cgagacgcac
    11041 gaccacgccg tcaaggccgg gcgcgtgcgc tgcctcgtgg cgggcggcat ggacagcggc
    11101 gcgctggcgc ggctctatgc cggcgtgccc ttggacacgc tgtacgaact gcagcagatg
    11161 atggaccagc tgatcgccga ttccatgggc aaggaatgcc ggctgaccga cgccaacggc
    11221 accgatgtgc gcttcgtgct gggcaaggcc accggcagca agccgcgccg cgcgctggcg
    11281 gcgggcgcca cctactcgct gcccggctcg gtcgtgatgt accccgattt gcccagcgtg
    11341 caaggccgca tcgtcatcgt cgccgcgatg cacgactggt acgggccgct ggagcggccg
    11401 ctgacgctgg aggtggacgg ccgcatccag cgcatcgtcg aagacggtcc ggacaccctg
    11461 ttgctcgatc gcgcgctgcg ccgggccggc ggcggcgatt acgggcacgt gatccatttt
    11521 tcctacggtt tgcatcccgc cgcgcgctgg cgcggcaatt gcttcgtgga agacatccgc
    11581 accgtcggcg ccaacgccat cggtttcggc cgcccctggt gggaaccggg cggcggcgag
    11641 aaccaccccg acggcttgct ggtgcgccag aacctctgga tcgatgggcg gcaggtcctg
    11701 cagtccggca tggtcgtcgc cccatcgtcc atggccgccg tggcgcgccg cgtcgccgac
    11761 gcggcatcgc cgggctgatg cggcttgctc gccgtacccg ttccgttcaa caggagagac
    11821 attcatgatt tgctacccat tcggcgtcgg tcccgtcgta acccggatat tcgaggcggg
    11881 ggcttccgac gcgcccgtga tcctgctctt gcatggcctg agttcgcgcg cggaccgctg
    11941 gatacgaaac atcgacgcct tggccgcggc cggctaccgc gtgatcgcgc cggacttgcc
    12001 cggccatggt ttcgccacca aggatccggc ccaggaccac agcattcccg gctatggaag
    12061 cttcgtgctg ggcctgctcg acgcgatggg catcccgaaa gcgaccatcg tcggcacgtc
    12121 gctgggcggg cacattgtcg ccaacgccgt gttgcgccag cccgagcgcg ccgaacgctt
    12181 gatgatgatc ggcagcaccg gactcgcccc cactgcgccc gcccgcgcgc agggcatgcg
    12241 cgattggctc atgaacctga cgcccgactc gcaccggccc aagctgcgca atgtttttac
    12301 cgacaaatcg ctggccagcg acgacatggt cgtggaagac gtgcggatca acacctcgcc
    12361 cggcgcctcg gcgtgcttcg atgccttcct ggcgtacatg gccgacggca tcaacgacga
    12421 tctcgtgctc gagcgcctgc cggagatcgc cgagcgcgtg ccgctgttgc tgttctgggg
    12481 cgtggacgac agttcggtgt cggtggacgt tggccgcgcc gcgcatgcca ggctgcccaa
    12541 gtcgaggctg attacggtgg agcacctgaa ccacacgccg tattacgaaa acccgccgct
    12601 cttcaacgaa gtgctgctca agttcatcgc cggcaggatc gacgatgtcc aggcgcccgg
    12661 cctgaccttg agctaagggc ggggatcgtt ccacgcggcc gattcctggc ggcgggaggc
    12721 gctaaggata ctggtatatg cagcgggccc gtccggcctg cttggcctgg agcagcgcgc
    12781 cgtcggcgcg ctgcagggcc tgggcgtaat gctcgtttgg cgtgtattgc gtcgcgccga
    12841 cgctgctggt gatgcggatg atggccgcct cggcggggat ggccagttcg gcaaccgttt
    12901 ggttgaaatg ctcaaccacg tgcctggctt ctgccagccc gacatcgggc atgatgacca
    12961 ggaattcctc gccgccccag cggccgcaga tatcgccttc ccggatcgag gacatcaagg
    13021 cctgggcaat ggccttgagg accaggtcgc cggccgcgtg cccccaggta tcgttgatgc
    13081 gcttgaagtt gtcgacgtcc aggatggcca ggcagtagga gcggccggtg cgctggaccc
    13141 gttggctttc ttcctgcagg caccgggtca gcagggcgcg gttgccgatc ccggtgagcg
    13201 ggtcgtgggt cgaggcgatc ttcaggacgc ggttctgctc ctgcaggcct ttcagcgctt
    13261 cgcgcaaggg atgcccggca tatcgcggat cattcaacaa ctgatcaacg agcctggtca
    13321 ggacgtcgtt ttccatggat taacgaggca agcctgagac agcggcttcc ggaaaggagc
    13381 ggctcatgga tatgaatatt aacaacctat taggttgagt aacaacctta tatgtccttc
    13441 atctcgatag gggggtgtcc gactatccca gtcggatagg gagggggcat ggccagtaca
    13501 gacctgacga atttgaccaa aagtgtcggg caggcaatcg caaagcgtcg aaccggttgt
    13561 gggttgactc aggagcaggt cgccgaacgg ctgggcatcg gcgtggaggc ggtgtcgcgc
    13621 atcgagcgcg gcgtggtgct gccgacggtt gcccgcctgg gagagttcgc cgacattttc
    13681 gagtgcaata tcgccgacct ggtcacggaa acgagttccc gggtcacgga tcaggccagc
    13741 cacctggcgg gcctgctctc caggctgggc agcgcggacc gcgccttggt cctggatatc
    13801 gtcgaaaccc tgattgcccg gctgggccgc ccgcggcact agccctgggc ctggccgaga
    13861 tcgggggcca tgatcgtggt ggccgcgcgg tccgcctcgg taatgatcca gatggcgttc
    13921 gtgccggaga gggcatagcg cgacagcacc ctcaggccga gcagcacggc cagttcgttt
    13981 tgcaatgcgt cttttgcggt gcagtccccc cagtcgccat ataaatggcg ggcaagcagg
    14041 aaaacgcctt ccgcgccggc ttttgccagg gcggccgatg ccgagtcggt gatcgacact
    14101 ttgcccagcg tgaggcgcgg gccggacgag gccggcgctg ccgcggtgtg cgccatgtgc
    14161 atgctcccta gaaaagcctg ccggatttca ggccatcggg agctatgttg aaggaaaaaa
    14221 tgtcccgcgg gatgctaggt ggatgttttc atccacggcc ggccgcaggc cgcgcgggcg
    14281 cctcgcaggc tcagggccag gccgatgcat agcgccagca tgacgaaggc atagccgccg
    14341 acggcattcc agccgcccat ctgccagaac ccgccgccga tcgatcccag gacgctcgag
    14401 cccaggtagt aggcgagcag gtacagggcc gaggcatggc ctttcgcgcc ttcggcgatg
    14461 ccgcccaccc agccgctggc gaccgagtgc gtgatgaaga acccgatggt cagcaagacg
    14521 atgccggcaa ccaccggcag gagcgtatgg gccagcgtca aggcaaggcc ggcggccgtg
    14581 accaggatgc cgatgcgcaa gacgggggga cggccgaagc gatcggccag cgcgccggcc
    14641 agcgaggacg cgacgatgcc gaacagatag gcgcagaaga tcaggctggt ctgcgtgggg
    14701 gtgaggctga agggcgcggc cgccagccga aagcccgcat agttgtatat cgtgacgaac
    14761 acgcccatca ccaggcagcc cacggcgaac agcttgcgca gcgcgctgtt ggacaggtgg
    14821 cggcgccaca gcgtgaaatg atggctgaag cccaggttgc ggctggccac gaaatgccgc
    14881 gacgccggca gcatcaggac gaaggcgagc gcggcggcga ggtccacgat gctgatggcc
    14941 atcagcgcgg cgcgccagga catggcttcg cccagcatgc ccatgccgac gcggcctatc
    15001 atgccgccga atgccgtccc gccgacatag aggcccatgg tcagccccag ggaggcggga
    15061 tggatttcct ccgcgaggta ggccatggcg acggccggca cgccgcccag cgccaggccg
    15121 cccagggcgc gcatgaccag caggccgtgc caggtgggcg cgcaggcggc gagcagattg
    15181 aaaaccgcgg cgagcgtcat ggagacgaac atcaggccgc gtcgcccgac ccgctgcgaa
    15241 agcgccccgg cgcagaggat ggaaatagcc agcaggccgg tcgataggga cagggccagc
    15301 gaacttgctg cgggaccgat ggcgaattcc tcggtgaaca tcggcagcag cggctggacg
    15361 caatacagca aggaaaacgt ggagaagccg gccaggaaaa gggcgaagcc gatgcgccgg
    15421 taggcgggcg ttccgcgcat gacccaggtc gtggcttcgg cttggggcgc ggacggttct
    15481 gtcagggact gctggttcat ggaaaaggca ggccggcata gggctcgacc atgcagccgt
    15541 atttttgcct tcccaagtta ttctgtccaa tatataaaac agggtatctc catatgtttt
    15601 gaaactatca tggaattgcg ccatctccgc tatttcctgg ccatcgcggc ggaagccaac
    15661 ttcacccgcg ccgcgcagaa gctgggcatc ggccagcctc ccctgagcca gcagatcaag
    15721 ggcctggagc gggagctggg ggtcgagctg tttcgccgca ccgcgcatgg cgtggtgctg
    15781 accccggccg gcgaggcttt cagggacgag gcggcgcgcg tggtcgaggg cgcgcaggac
    15841 gcggtacgcg ccgcgcagcg cgcggcacgc ggagaaactg gccgcatgcg gctcgggttt
    15901 accggctcgg cggccttcaa tcccgtggtg tcggaattga tccggcagtt caaccgggac
    15961 tatcccaagg ccgacctggt gctcgtcgag gacaacacga tgcagttgct gcaggcgctc
    16021 gccgacaacc ggctcgacgc catcttcttc cgtcccgagc tgggcgggtc cgagggactg
    16081 cggctgcatc gtttccagga cgagcccatg aagatcgtgc tgcccgcccg ccatccgctt
    16141 gccaggcacc gcgcgctgcc gttgtcggcg ttggccggcg aggctttcgt catcgtgccg
    16201 ggctcagccg gcgccacgtt gtacaacgag atcatgaacc tgtgccgcga gcttggcttc
    16261 gaaccggcgc tcgcccagcc cgcgccgcag gtgtcgtcgg tcatcaacct cgtcgccgcc
    16321 gggctgggca tttccattgt gccggccgcc ctggcacagg tgcgcgtggc gggcgtgcgc
    16381 tatctcgaca tcaggccgcc ggccccggtc gccagattgg cgctggccgc gcgcgccgac
    16441 aatgcctcgg tggtggtccg gaactgcttc gagttggcgg ccggcatcta gcgcagggcc
    16501 ggatgccggc ctgcctacca gctcgcatcc agcccgagct gccgcagggc gcgctgccgc
    16561 atcgccacca ggcgcggatc gccgcgatgg cgggggtagg gcaggtcgtt gatgatttcg
    16621 tccttgatgc gcgcgggccg gtcgctcagc acgatgatgc ggtcggccag gaacagcgct
    16681 tcctcgacgt cgtgcgtgac cagcagcacg gtgatgccgg tgcgccgcca gagttccaac
    16741 agttcggact gcatggccag gcgcgtcagg gagtccagct tgcccaaggg ctcgtccagg
    16801 atcaggatgc gcggatcgtt gaccagcgcg cgcgccaggg cggcgcgctg cgccatgccg
    16861 cccgacagct ggtgcggata ggcgccggcg aattccgcca gcttgacgcg ctccagggca
    16921 tcgtcgatgc gggcgttccg ggcgggcagc agcccgcgcg cctgcaggcc gagggccacg
    16981 ttgtcgcgca ccgtgcgcca gggatacagc gtggggtcct ggaacaccag gatgcggctc
    17041 gggtcggggc gcaggatttc ctcgccgtcg gccgcaatag aaccggaact cggcgtctcc
    17101 aggcccgcga ccaggcgcag cagcgtgctc ttgccgcagc cgctggggcc cagcaaggcc
    17161 aggaactcgc ccggccgcac ctccaggtcg atattcgaca acaccggcag cgacgagccg
    17221 cccagatcga aagcgtggtc gacgcggcgg agggagaccg cggcgccggc cgcttgcacg
    17281 gcctctacca tttcacgact cctttctgcc aggccagcag ccggtcgcgc accacgaaca
    17341 ggagcgtgat gatgccggag aaggtcacgg ccatgacgat cagcgcgccg tacatattgg
    17401 cgtacgaggc ccatccttga gcccacgaca ggtaccagcc caggccggat ttcacgccca
    17461 tcatctcggc gacgaccagc accgagaagg cgtggcccag gcccatgaac aggccgacga
    17521 acacatgcgg cagggcggcg ggaatcgcga cgcggaagat caggaaggcc tggctcgcgc
    17581 ccagggtgcg cgccacgtcg taataggccc ggttcacgct ggccacgccc gaccaggtaa
    17641 gcacggtgac gggaaaccac gtcgacagcg cgatgaggaa aaccgccgcg gaaaaactcg
    17701 acgggaagac aaaaaaggag atcggcagca aggcggtgga cggcaccggc cccagaaagc
    17761 gcagcaccgg atgcacccag tagcccagcc tgcgcgacca gccgatggcc acgccggtga
    17821 cgaagccgaa gaaagcgccc agcgcgtagc cgttggccag cagccagagc gaatgccaca
    17881 ggctgtcgag caggcgcggc cagtcctgcg cgtagacctc gatcagcgcc tggggcggcg
    17941 cgaagaacgg cgaaggcagc agggccagct tcgcggtggc gatttcccag aggccgatcg
    18001 ccacggccag cagcgccagc cacgggcccg ccaggcgcag gccgcgcaat aaccggttcg
    18061 gcgagcggct cgccaggccg gaaaccgcca gcgccgccag caagaccgcg atcgccagcg
    18121 cggccgtgcc gaaggcgtcc gtgtagcgcc attccttgaa gcccacctgg aggtcgggcc
    18181 acaacagcgt gaccaggccg gtggccgccc acaacacggc ggccagcaag ccggacggcc
    18241 agaagcgggc cagggccgtg ccggacaggc gggggctccc cggactcggt ctcgcgtcgt
    18301 gcgcgttgac cgcgagttcg gtgtgcgggg gcatggctat acgtcggcgg taacggccgc
    18361 ggcgaatttc tgcggatcga tgccggggct gatgacgtgg atggccttga gctcttccac
    18421 gtagtcggcg atttcctgct tgaggggctg gtgcaccggg tgatggtgat gcgacatgcc
    18481 ggccagcatt tccaccagat cgtcgacggg aaccttgggc gagtagctgg cgaacagctc
    18541 cgccgccttg cgcgggtttt ccgccgtcca ctcgctgcct tcctgtatgg cggccgcgag
    18601 cgcctgcgcg gtcttgaagt cattgcgcac catattgccc gacacggcca gcacgcagca
    18661 ggatcggtcg gcgtacttgc cggtcatgtt ggaggcgagc tctctcaggc cgaaattctt
    18721 gcggaccagt gaaatgatgg ggtcgttgtc ggcgacggca tggacctcgc ccttttgcag
    18781 gccttgtcct agcaggtcgg ccggataggc gcgccacgcc acgtcttttt cagggtcgac
    18841 gcccgcctgg ccgagcagga tggaaaagaa attcttcgcg ggactggcca tgtcgctcac
    18901 gccgatggtc ttgcccttga ggtcggccac ggaggcgtag gggctgtcct tggcgacgag
    18961 caggcggatg cagccggaca tcacgcccga gatgatcttg acatcgaagc cctgctcgag
    19021 cggcttgagc cagcgcaggg ccatgccgac gccggcctgc gccttgccgg acgcgatggc
    19081 ttcgagcaac tggtcggtcg agccggcgaa attgatgaag ctgacgtcca ggccgtactt
    19141 gcggaagtag ccctgctcca gggcggccgg aacgggcacc gtgcagacgg ccgtcgcgct
    19201 ccaggcgatg gaaaccttct tcaagggctc ggccggctgc gccagcgcca ggccggtttt
    19261 gcccgccatg atcgcggccg tggcgcccag cgagccttgc aggaagcgcc gccgcgccgc
    19321 ccacgcggcg ctctggttca tatgccattc atgtatggat ctagccattt tgatagtgcc
    19381 gttttttgaa aggggaacgg cgttgattct aggtagctgg gcgcggagcg gttaaacaaa
    19441 attcgcatgt gctaatgggg aatcgaccat gcaagggagc gcttcctggc gggtagtatt
    19501 agcctgacat gaaggcaaaa aaggcgatgc taagatcgcg ggagaaacca tgaccactct
    19561 ttccagccag cccgcccgtg cggatgccgc cgcaacggcg ccgctgcgcg aattcatcgc
    19621 atcgctttcg ggcctgctcg accgacagcc cgccgaagcg caaatcctgc gggagggcgg
    19681 cgccctgctg gccaggctgg tcgcccggga cggctggctg ccggacgaat atgccgtgcc
    19741 caacccggat cgctaccagc agttcctgct gcacatggat tcggcgcagc gcttctgcgt
    19801 ggtgagtttc gtctgggggc cggggcagag cacgcccatc cacgaccata cggtgtgggg
    19861 gctgatcggc atgctgcgcg gggcggaata cagccagccc tatcggctgc atgaagacgg
    19921 cggcgcgcgc gagcacggcg ccgccatcag gctcgagccc gggcaggtcg aggccgtctc
    19981 gccgcgcgtg ggcgacatcc acaaggtgag caatgcctac gccgaccgcg cgtcgatcag
    20041 catccacgtg tatggggcca atatcggcgc ggtgcgtcgt tcggtctatg cggtcgatgg
    20101 cgcgcgcaag cctttcatct ccggctattc caatacttcc ctgcccaata tctgggacct
    20161 ttccacgatg tcgaagccat catgagcagc ccggacgcaa gctttccctc gacctcgtat
    20221 gcccaggtgc ggcgcgcttt gatcgacggg acggaaatcg ccctgatgga tgtgcgcgag
    20281 gaagatccct ttgcgcagga acatcccctg tgggcggcca actttccctt gtcgcgcctg
    20341 gagatcgata cctgggagcg cattccccgg cgcgataccc gcatcgtggt ctacggccat
    20401 gacgagtcgg gcgacctggc gccgcgcgcc gcggccgtgc tgcgcgccca gggctatgcc
    20461 gacgtgagcc tgctggacgg cggcctggaa ggctggcgcg cggcgggcgg cgagctgttt
    20521 cgcgatgtga acgtgccgag caagtccttc ggcgagctgg tggaggccac gcgccacacg
    20581 ccgtccttga gcgcgcagga agtcggccag ttgatcgagg acggcgccgg cacgctggtg
    20641 gtcgacgtgc gccgctatga cgaataccac accatgagca tcccctcggc taccagcgtg
    20701 ccgggcgcgg aactggtgct gcgcatccgc gaactggcgc ccgacccgtc gacccgcgtc
    20761 atcgtcaatt gcgcgggccg cacccgcagc atcatcggcg cgcagtcgct ggtcaatgcc
    20821 ggcataccca atccggtggc ggcgctgcgc aacggcacca taggctggac cttggcaggc
    20881 ttggaactgg cgcagggcgc gacggcgcgt gcccccgaag taggggcgga ggtggtggcc
    20941 ctggcca
//
LOCUS       DASWPO010000062         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1888449, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000062 DASWPO010000000
VERSION     DASWPO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888449"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /gene="trbJ"
                     /locus_tag="VGC69_03895"
     CDS             complement(<1..421)
                     /gene="trbJ"
                     /locus_tag="VGC69_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HEY0294461.1"
                     /translation="MKTKPRLLSASLAAALSVSLMLAQPAAAITVFDPSNFVQNTLTA
                     VRTLEQINNQINQLQNEAQMLMNQARNLARLDFNIVNRLRSTLATTERLIAEAQGLAF
                     DVTNMDREFARLYPEQYAATVSGDRMAQDARERWQNSL"
     gene            complement(418..2868)
                     /gene="trbE"
                     /locus_tag="VGC69_03900"
     CDS             complement(418..2868)
                     /gene="trbE"
                     /locus_tag="VGC69_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HEY0294462.1"
                     /translation="MLNLAEYRQRPALLADWLPWAGLIAPGVVLNKDGSFQRAARFRG
                     PDLDSATQGELIATSARLNNALRRLGSGWALFIEAERRPAADYPHSDFPEPLSWLVDE
                     ERRAAFEESGHHFESAYHLTLAYLPPEESRARAAKLLYENAPGDGVDWRGRLDAFVAE
                     TDRVFDLLDGVMPEIAWLDDAQTLTYLHATVSTRRYPVAVPEVPFHIDALLAYSALVG
                     GLAPTLGDQHLRVVSVRGFPTSTWPGLLDDLNRLGFAYRWSTRFLCLDKAEAEKELGR
                     LRRQWFAKRKNVIALLRETIFQQESPLVDTDASNKAADADAAMQELGSDQVAFGHFTA
                     TVTVLDADPAAADEKLRMVERVIQGRGFVTIPETLNAVDAWLSSIPGNAYANVRQPIV
                     STLNLAHMMPLSAVWAGPGKNDHLDGPPLIVTRTDGATPFRLVTHIGDVGHTLVAGPT
                     GMGKSVLLAILAMQFRRYFGSRIFAFDMGRSMRATILGLGGEHYDLGADGGIAFQPLA
                     RIDRESYRSWAGEWVEGRLLHEGVTVGPDEKAVIWSALGSLAGAPVEQRTLTGFSVLL
                     QSNALRQALAPYVLGGAHGKLLDADTDRLGFGDVQGFEMEELMHSPAAVQAVLRYLFA
                     RFDERFDGAPTLLILDEAWLFLDEPSFAARIRQWLKTLRKKNVSVIFATQSLADIKDS
                     SIAPAIIESCPSRIFLPNPQVTEPQIRTIYEGFGLNSRQIEIVATAQPKRDYYYQSRL
                     GNRLFDLDLGPAALAFAGASTPQDQRDIDRVLTQAGAPGFAGAWLRHRGLDWAADLLP
                     SAPAAASFLNSQPQEVSP"
     gene            complement(2892..3164)
                     /locus_tag="VGC69_03905"
     CDS             complement(2892..3164)
                     /locus_tag="VGC69_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HEY0294463.1"
                     /translation="MSAPDAFAAGFEVPLHRSLTEPILLGGAPRTVAIANGTLAAAVG
                     LGLQLWIPGAVLWIVGHSLAVWGARVDPQFMQVFARHIKHKPLLDV"
     gene            complement(3161..>3276)
                     /locus_tag="VGC69_03910"
     CDS             complement(3161..>3276)
                     /locus_tag="VGC69_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HEY0294464.1"
                     /translation="TSGGFRKLIQIVFGLSIAFAASSFFLSFFSFSGGAVV"
BASE COUNT          580 a         1058 c         1121 g          517 t
ORIGIN      
        1 tcaagctgtt ctgccagcgt tcgcgggcgt cctgcgccat gcggtcgccg ctgacggtgg
       61 cggcgtactg ctcgggatac aggcgcgcga actcccgatc catgttcgtc acatcaaagg
      121 ccagcccttg cgcctcggcg atcaggcgtt cggtggtggc gagcgtcgag cgcaggcgat
      181 tgacgatgtt gaagtccaga cgcgccagat tgcgtgcctg gttcatgagc atctgtgctt
      241 cgttctgaag ctggttgatc tggttgttga tctgttccag ggtgcgcacg gccgtcagcg
      301 tgttctgcac gaagttcgac ggatcgaaca cggtgatggc cgccgctggc tgcgcgagca
      361 tcagcgagac agacagcgcg gcagcgagcg aggcggacag caaacggggc ttggtcttca
      421 tggtgaaacc tcctgtggct gggagttgag gaacgaagca gccgccggtg cggacggcag
      481 caggtcggcg gcccaatcaa ggccgcgatg gcgcagccac gcaccggcga agccgggagc
      541 gccggcctgc gtcagcacgc ggtcaatgtc gcgttggtct tgcggtgtgg atgcgcccgc
      601 gaaggcgagc gcggccggcc ccaggtcgag gtcgaacagg cgattgccga gacgcgattg
      661 gtagtagtag tcgcgcttgg gctgggcggt ggcgacgatc tcgatttgcc ggctgttgag
      721 gccgaagccc tcgtagatcg tgcgaatctg cggttcggtc acctgcgggt tgggtaagaa
      781 aatccgactc gggcagctct cgatgattgc gggcgcaatg ctcgaatcct tgatgtccgc
      841 cagactctgc gtggcgaaga tcacggacac gtttttcttg cgcagcgtct tcaaccattg
      901 gcgaatgcgc gcggcgaaag acggctcatc gaggaacagc cacgcctcat ccagaatcag
      961 tagcgtcggc gcaccgtcga aacgctcatc gaagcgggcg aacaggtagc gcagcaccgc
     1021 ctgcacggcg gcggggctgt gcatcagttc ttccatctcg aagccctgca cgtcaccgaa
     1081 gccgagacga tcggtgtccg cgtccagcag cttgccgtgc gcgccaccga gcacataggg
     1141 cgcgagtgcc tgccgtagcg cgttcgattg cagcagcacc gagaacccgg tcaacgtgcg
     1201 ctgctcaacg ggcgccccgg ccaagctgcc cagcgccgac cagatgaccg ccttctcgtc
     1261 cgggccgacc gtcacgccct catgcagtag gcggccttcc acccattccc ccgcccagga
     1321 gcgatagctt tcccggtcga tacgcgcgag tggctggaag gcaatgccgc catcggcacc
     1381 caggtcgtag tgctcgccgc ccaggcccag gatggtggcg cgcatcgagc ggcccatgtc
     1441 gaaggcgaag atccgcgagc cgaagtagcg gcggaactgc atcgccagga tggcgagcaa
     1501 gaccgacttg cccatgccgg tcgggccggc gaccagggta tgccccacgt cgccgatatg
     1561 cgtcaccagc cggaacggcg tcgcgccatc ggtgcgggtg acgatcagcg gcgggccgtc
     1621 gaggtggtcg ttcttccccg gcccggccca caccgccgac agcggcatca tgtgcgccag
     1681 gttcaacgtc gagacgatgg gctggcgcac gttggcgtaa gcgttgccgg ggatggatga
     1741 aagccacgca tccacggcgt tcagggtttc gggaatggtc acgaagcccc ggccctggat
     1801 gacgcgctcc accatgcgca gcttttcgtc ggccgcggcc ggatcggcat caagcaccgt
     1861 caccgtggca gtgaagtgac cgaaggccac ctgatcgcta cccagctcct gcatcgcagc
     1921 atccgcgtcg gccgccttgt tgctggcgtc ggtatcgacc agcgggcttt cctgctggaa
     1981 gatcgtctcg cgcagcagcg cgatgacgtt cttgcgcttg gcgaaccatt ggcggcgcag
     2041 gcggccgagt tccttttctg cttcggcttt gtccaggcag agaaagcgcg tactccagcg
     2101 gtaagcgaag cccaggcggt tgaggtcgtc cagcaggccc ggccaggtcg aggtcggaaa
     2161 accgcgcacc gacaccacgc gcaggtgctg gtcgcccagc gtgggcgcca ggccgccgac
     2221 cagcgcggag taggccagca gcgcgtcgat atggaacggc acctccggca cggccaccgg
     2281 atagcgccgc gtggacaccg tggcgtgcag gtaggtcagc gtctgggcgt catcgagcca
     2341 ggcgatttcc ggcatcacgc catcgagcaa gtcgaagaca cgatcggttt ccgccacgaa
     2401 ggcatcgagc cggccgcgcc agtccacgcc gtcgccaggc gcgttctcgt agagcagctt
     2461 ggccgcgcgg gcgcgcgatt cctcgggcgg caggtaagcc agcgtcaggt gataggcgct
     2521 ctcgaagtga tgccccgatt cctcgaaggc ggcgcggcgt tcctcgtcca cgagccagga
     2581 cagcggttcg ggaaagtcgg aatgcgggta gtccgccgcc ggccggcgct cggcctcaat
     2641 gaacaaggcc cagcccgaac ccaggcgacg cagcgcgttg ttgagccgtg ccgacgtggc
     2701 gatcagttcg ccctgcgtcg cgctgtccag atcaggcccg cgaaagcgcg ccgcgcgctg
     2761 gaatgagccg tccttgttca agacgacgcc cggcgcgatc aggccagccc agggcaacca
     2821 gtcggccagc agcgcggggc gctggcggta ttcggcaagg ttcagcatgg cgcttatctc
     2881 ctttctcgcc cctacacgtc cagcagcggc ttgtgcttga tgtgccgggc gaagacctgc
     2941 atgaactgcg gatcgacgcg cgcgccccag accgccagcg aatggccgac gatccagagc
     3001 accgcaccgg gaatccacag ttgcaggcct agcccgacgg cggcggccag cgtgccgttg
     3061 gcgatcgcca cggtgcgcgg cgcaccgccc agcaggatcg gctcggtcag cgagcgatgc
     3121 agcggcacct cgaaacctgc cgcgaaggcg tccggggcac tcatacgacg gccccgccgg
     3181 agaacgagaa gaacgacagg aagaacgagg acgcggcgaa cgcgatggac aggccgaaca
     3241 cgatctggat caacttgcgg aagccgccgc tggtat
//
LOCUS       DASWPO010000063         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1351985, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000063 DASWPO010000000
VERSION     DASWPO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351985"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1250)
                     /gene="ilvC"
                     /locus_tag="VGC69_03915"
     CDS             complement(234..1250)
                     /gene="ilvC"
                     /locus_tag="VGC69_03915"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY0294465.1"
                     /translation="MKVFYDKDCDLSLIKGKTVAIIGYGSQGHAHALNLHDSGVKVVV
                     GLRKGGASWSKAANAGLEVAEVAEAVKRADIVMMLLPDENIASVYSKEVQANIKPGAA
                     LAFAHGFNVHYGQVVPRDDIDVIMIAPKAPGHTVRSTYSQGGGVPHLVAVYQDKSGSA
                     RDVALSYASANGGGRAGIIETNFREETETDLFGEQAVLCGGTVELIKAGFDTLVEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEFGEYETGPKIVTDETRQAMRQ
                     ALKDIQTGEYAKRFILENTAGAPTLTSRRRINAESQIEVVGTKLRAMMPWIAANKLVD
                     KSKN"
     gene            complement(1346..1837)
                     /gene="ilvN"
                     /locus_tag="VGC69_03920"
     CDS             complement(1346..1837)
                     /gene="ilvN"
                     /locus_tag="VGC69_03920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY0294466.1"
                     /translation="MKHVISVLMENEPGALSRVVGLFSARGYNIETLTVAPTEDATLS
                     RLTVVTEGPDEVIEQITKHLNRLIDVVKVVDLTEGPHIERELMLIKVRAVGKEREEVK
                     RMADIFRGRIIDVTDKSYTIELTGDQGKNQAFIEALDRSAILETVRTGVSGIGRGERI
                     LKI"
     gene            complement(1848..>3335)
                     /gene="ilvB"
                     /locus_tag="VGC69_03925"
     CDS             complement(1848..>3335)
                     /gene="ilvB"
                     /locus_tag="VGC69_03925"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY0294467.1"
                     /translation="GVTNAITGIATAYMDSIPLVIISGQVPTAAIGEDAFQECDTVGI
                     TRPCVKHNFLVRDVKDLAETMRRAFYIARTGRPGPVLVDIPKDITVAVGKYAPPKGEI
                     GMRSYAPVNKGHQGQIKKAVQMLLTAERPMVYTGGGVVLSNAAPELRRLADQLNAPVT
                     NTLMGLGSMPGTDPRFLGMPGMHGTYEANMAMQHCDVLLAIGARFDDRVIGNPRHFAQ
                     TARKIIHIDIDPSSISKRVRVDVPIVGNVKDVLHDISALYEAASVETKPASLKKWWEQ
                     IHAWRGKECLKYANSDEIIKPQFVVEKLWEVTGGKAFVTSDVGQHQMWAAQYYKFDEP
                     RRWINSGGLGTMGVGLPYAMGVQMANPGSDIAVITGEASIQMNIQELSTCQQYHFTPK
                     IVCLNNRFLGMVRQWQQIDYGSRYSESYMDSLPDFVKVAEAYGHVGLRIEKPADVEPA
                     LREAFKKHKDRLVFLDFITDRTENVWPMVKAGRGLTEMLLGSEDL"
BASE COUNT          513 a         1044 c         1127 g          651 t
ORIGIN      
        1 ccactggcgc ctgcgcttcg gatttctccg gggtcctcgg ctagcaacag cccaatgcgg
       61 cggtgttaac cccgcaactg cgagtgccgt gagccgtcgc cagggcgcag ggagccgaag
      121 cgcaggcgcc agtggcgccg agcatcggaa tcccgcagtc ctggcgacgg cggctcaaga
      181 acccgacagc gccgctggcg tcagcaccct cgacaaacgc caacagccca cgatcagttc
      241 ttgctcttgt cgaccagctt gttggccgcg atccagggca tcatggcgcg cagcttggtg
      301 ccgacgacct cgatctgcga ctcggcgttg atgcggcggc gtgaagtgag cgtgggcgca
      361 ccggcggtgt tttccaggat gaagcgcttg gcgtattcgc ccgtctggat gtccttgagc
      421 gcctggcgca tggcctggcg ggtctcgtcg gtgacgatct tggggccggt ctcgtattcg
      481 ccgaattcgg cgttgttgga gatcgaatag ttcatgttgg cgatgccgcc ttcgtagatc
      541 aggtcgacga tgagcttgag ctcgtgcagg cactcgaaat aggccatctc gggcgcgtag
      601 ccggcttcca ccagggtgtc gaagcccgcc ttgatcagct cgaccgtgcc gccgcacagc
      661 acggcctgct cgccgaacag gtcggtttcg gtttcttcgc ggaagttggt ctcgatgatg
      721 ccggcgcgcc cgccgccgtt ggcgctggcg tacgacaggg ccacgtcgcg ggccgagccc
      781 gacttgtcct ggtagaccgc caccaggtgc ggcacgccgc cgccctggct gtaggtggag
      841 cgcacggtgt ggccgggggc cttgggcgcg atcatgatga cgtcgatgtc gtcgcgcggc
      901 acgacctggc cgtagtgcac attgaagccg tgggcgaagg ccagggccgc gcccggcttg
      961 atgttggcct ggacttcctt gctgtagacg ctggcgatgt tctcgtcggg cagcagcatc
     1021 atgacgatgt cggcgcgctt gacggcttcg gccacctcgg cgacttccag gccggcgttg
     1081 gcggccttgc tccaggaggc gccgcccttg cgcaggccca ccaccacctt cacgcccgag
     1141 tcgtgcaggt tgagcgcgtg ggcgtggccc tgcgagccgt agccgatgat ggcgaccgtc
     1201 ttgcctttga tgagggagag atcgcagtcc ttgtcgtaga aaaccttcat gtttgtgctc
     1261 cgtgtatgta ggaatagcgg gccgggatca tccggcgtcg tggggggttc gaaaaaatag
     1321 gaaatggcgt ggcgccgggc gccggtcaga tcttgaggat gcgttcgccg cggccgatgc
     1381 ccgacacgcc cgtgcgcacg gtttccagga tggcgctgcg atccagcgcc tcgatgaagg
     1441 cctggttctt gccctggtcg cccgtgagct cgatggtgta ggacttgtcg gtcacgtcga
     1501 tgatgcggcc gcggaagatg tcggccatgc gcttgacctc ttcgcgctcc ttgccgaccg
     1561 cccgcacctt gatcagcatg agttcgcgct cgatgtgcgg gccctcggtc aggtcgacca
     1621 ccttcaccac gtcgatcagg cggttcaggt gcttggtgat ctgttcgatc acttcgtcgg
     1681 gcccttcggt gaccacggtc aagcgcgaca gggtcgcgtc ctcggtgggc gccacggtga
     1741 gcgtctcgat gttgtagccg cgcgcggaaa acagccccac cacgcgcgac agcgcgcccg
     1801 gttcgttttc catgaggacg gaaatcacgt gtttcatacc agcctcctca cagatcttcg
     1861 gaaccgagca gcatttcggt cagcccgcgg ccggccttca ccatgggcca cacgttctcg
     1921 gtgcggtcgg tgatgaagtc caggaagacc aggcgatcct tgtgcttctt gaacgcctcg
     1981 cgcagcgccg gctcgacgtc ggccggtttt tcgatgcgca ggcccacgtg gccgtaggcc
     2041 tcggcaacct tgacgaaatc cggcagcgaa tccatgtacg actcggaata gcgcgagccg
     2101 tagtcgatct gctgccactg ccgcaccatg cccaggaagc ggttgttcag gcacacgatc
     2161 ttgggcgtga aatggtattg ctggcaggtg gagagctcct ggatgttcat ctggatggag
     2221 gcttcgccgg tgatgacggc gatgtccgaa cccgggttgg ccatctgcac gcccatggcg
     2281 tagggcaggc ccacgcccat ggttcccagg ccgccggagt tgatccagcg gcgcggctcg
     2341 tcgaacttgt agtactgcgc ggcccacatc tggtgctggc ccacgtcgga cgtgacgaaa
     2401 gccttgccgc cggtgacttc ccagagcttt tccaccacga actgcggctt gatgatctcg
     2461 tccgaattgg cgtacttgag gcattccttg ccgcgccagg catggatctg ctcccaccac
     2521 ttcttgagcg aagccggctt ggtctcgacc gaggcggcct cgtacagggc gctgatgtcg
     2581 tgcagcacgt ccttgacgtt gccgacgatg ggcacgtcca cgcgcacccg cttcgagatc
     2641 gaagaagggt cgatgtcgat atggatgatc ttgcgcgcgg tctgcgcgaa atgcctggga
     2701 ttgccgatga cgcggtcgtc gaagcgcgcg ccgatggcca gcagcacgtc gcagtgctgc
     2761 atggccatat tggcctcgta ggtgccgtgc atgccgggca tgcccagaaa gcgcgggtcg
     2821 gtgccgggca tggagcccag gcccatgagc gtattggtga ccggcgcatt gagctggtcg
     2881 gcaagccggc gcagttcggg cgccgcgttg gacagcacca ccccgccgcc ggtgtagacc
     2941 atcggccgct cggccgtcag cagcatctgc acggccttct tgatctggcc ctggtgccct
     3001 ttgttgacgg gcgcatacga gcgcatgccg atctcgccct tgggcggcgc gtacttgccc
     3061 acggcgaccg taatgtcctt cgggatgtcg accagcaccg gtccggggcg gccggtgcgc
     3121 gcgatgtaga acgcgcgccg catggtctcg gccaggtcct tcacgtcgcg caccaggaag
     3181 ttgtgcttga cgcagggacg ggtgatgccg acggtgtcgc actcctggaa ggcatcctcg
     3241 ccgatcgccg ccgtgggcac ctgtccgctg atgatgacca gcgggatgga gtccatgtag
     3301 gcggtggcga tgccggtgat ggcgttggtc acgcc
//
LOCUS       DASWPO010000064        21488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2603417, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000064 DASWPO010000000
VERSION     DASWPO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 21488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21488
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2603417"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGC69_03930"
     CDS             <1..454
                     /locus_tag="VGC69_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY0294468.1"
                     /translation="FLESENPDKDISFYINSPGGSVYAGMAIYDTMQFVKPDVSTLCT
                     GLAASMGAFLLAAGKKGKRYTLPNSRIMIHQPSGGAQGQATDIQIQAREILDLRARLN
                     RILSESTGQPIDRIEVDTERDNFMSAEDAVSYGLVDKMLTSRADAAGV"
     gene            472..1764
                     /gene="clpX"
                     /locus_tag="VGC69_03935"
     CDS             472..1764
                     /gene="clpX"
                     /locus_tag="VGC69_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY0294469.1"
                     /translation="MPDKKGSVDAKVLHCSFCNKSQHEVRKLIAGPSVFICDECIDLC
                     NDIIREEAQASARAAIRSELPTPSEIKSFLDQYVIGQTSPKRMLAVAVYNHYKRIRHG
                     EIKGDEVELSKSNIMLIGPTGSGKTLLAQTLARMLNVPFVMADATTLTEAGYVGEDVE
                     NIIQKLLQSCNYDVEKAQRAIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEG
                     TVASVPPQGGRKHPNQDFVQVDTTNILFIVGGAFDGLEKVIRDRTEKSGIGFSASVRA
                     KSERGVGELFSEVEPEDLIKFGLIPELVGRLPVVATLDELDEAALVQILTEPKNSLVK
                     QFQKLFAMEGAELDVRPSALRAIATKALKRKTGARGLRSILEASLLDTMYELPSQGNV
                     KRVVLDESAVEGKGKPLLIYADAEGEGDPKASRGEAAA"
     gene            2036..4489
                     /gene="lon"
                     /locus_tag="VGC69_03940"
     CDS             2036..4489
                     /gene="lon"
                     /locus_tag="VGC69_03940"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0294470.1"
                     /translation="MSANQTLPAEPIDLPLLPLRDVVVFPHMVIPLFVGRPRSIRALE
                     VAMEAGKSIMLVAQKSAGKDDPTPEDVYEIGCVASILQMLKLPDGTVKVLVEGTQRAR
                     IDSIEDVESHFTCLVTPIEPDLAQSSESEALRRAIVAQFEQYVKLNKKIPPEILTSLA
                     GIDDPGRLADTIAAHLPLKLEQKQAMLEITGTTERLEGLLTQLETEIDILQVEKRIRG
                     RVKKQMEKSQRDYYLNEQVKAIQKELGEGEEGADLEELEKKIIAAHMPKEARKKADAE
                     LKKLKLMSPMSAEATVVRNYIDTVINLPWKKKSKVNNSVSHAETVLDNDHYGLEKVKE
                     RIVEYLAVQQRVDKLKAPILCLVGPPGVGKTSLGQSIARATNRKFVRMALGGVRDEAE
                     IRGHRRTYIGSMPGKILQNLSKTGTRNPLFLLDEIDKLGMDFRGDPASALLEVLDPEQ
                     NHTFQDHYVEVDFDLSDVMFVATSNTLNIPPALLDRMEVIRLSGYTEEEKIHIIRDHL
                     LPKLMKNNGVKENELEVDDSALRDIVRYYTREAGVRSLEREVGKVCRKVVKKLLVQQA
                     SNAKDGENAEPLILPVKVTSENLSDYLGVRRYTFGMAEKENQIGQVTGLAWTEVGGEL
                     LTIEVVDMAGKGAIQRSGSLGDVMKESVEVARTVVRSRARRLGFADSVFEKRDMHIHF
                     PEGATPKDGPSAGIAITTAMVSALSRIPVRADVAMTGEITLRGEVLPIGGLKEKLLAA
                     HRGGIKTVLIPEENVKDLAEIPDNVKNSLEIVPVRWIDKVLELALERLPEPLAEEDAA
                     KTDVVATQAAPAVDPVVKH"
     gene            complement(4710..5405)
                     /gene="lexA"
                     /locus_tag="VGC69_03945"
     CDS             complement(4710..5405)
                     /gene="lexA"
                     /locus_tag="VGC69_03945"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041937182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0294471.1"
                     /translation="MASKLTARQQEILDLIRAAVAHTGFPPTRAEIAQALGFRSPNAA
                     EDHLKALARKGAIELTAGASRGIRLLVGEGDIRTDAGLAASALASAGALIADSAAAFS
                     RLLLPLVGRVAAGSPILAAEHVEREIGVDPALFSQTPDYLLKVRGMSMRDAGILEGDL
                     LAVKRAAEARNGQIVVARIDDDVTVKRLQRQSGRIELLPENPDFEPIVVQPGQNFALE
                     GIAVGLIRTQALH"
     gene            complement(5517..6830)
                     /locus_tag="VGC69_03950"
     CDS             complement(5517..6830)
                     /locus_tag="VGC69_03950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0294472.1"
                     /translation="MTWHDRPPGEPAHAGNLWRATAGLPDGASPPLDGSAQADVAVVG
                     AGYLGLSTAMHLAESGRKVVLLEAGDIGFGASGRNGGQVNPGLKWGREELVHRFGPAG
                     DGFFRLGEEGPAFLAELVRRKNLDCNLSFPGVLRCAHNVRALKNLDAACADLRARGID
                     ARRLDAAQTEAHTGTTAYAGALLDMRGGSVHALALARGLARAAADSGAAIHCNSPVLA
                     AVRHGKQWELRTATGSVRAHGVVVATNGYTDQLVPGLARTLLPVNCFQIATGVLPAHL
                     QDSILPGGQAVYDSRRLILFFRKSPDGRLMLGGRASFSSQTGSGEQASDYHVLERVLH
                     GIYPQTRELPVEYRWRGLVCLTPDFLPHYHRPADGMHVLVGMNGRGVALATRAGAWLS
                     DTILGKTGDTGVPATPIRPIALHGLRAPVLNAVMKWNWLMDLAGR"
     gene            complement(6827..8482)
                     /locus_tag="VGC69_03955"
     CDS             complement(6827..8482)
                     /locus_tag="VGC69_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0294473.1"
                     /translation="MTSSLDPIQLSILNQALRSITEEMGANLVRSSFSTVIREARDCS
                     TAILTPDGEVVAQSALIPMQTVALSEAFHGASQRVDLGRLTDGHAMLMNDPYAGGQHL
                     NDLILFQPVLFDGALLGYVGSTAHHIDIGGADAGINVRATHLLQEGIVISPMIFNIRN
                     DWEHGNLESLICANVRLPDILRGDLNAQFAANHIGIERLSALARREGPDTVRQAMDAI
                     LDHGETRMRQALARIPDGVYHGSARMDRDVFDPIPIEVKVKVTVSGSDIELDYAGTHA
                     QVASMFNCPPASSRAAALSAIRCLVSDQKLPSNDGCNRPIVSRFPEGSILNPHWPAPV
                     RARAIVAYRAFDAILVALAGAMPEQATAPGFNTTTAVYISQQRQDGSTRIYGDIFGGG
                     LGAGQGYDGADAVDAIMSNCRCTPIEVIEHAHDHLRVASFAIEPDSGGAGQWRGGLGF
                     QRTFDILEDGADVSIYSDRFRFAAQGLHGGTPGGLGALEVLRDGEKIRLDAMSTFPLK
                     RGDRLTIRVGGGGGWGDPARRASEAIQTDLVDGWVTHPPGVSA"
     gene            complement(8479..10539)
                     /locus_tag="VGC69_03960"
     CDS             complement(8479..10539)
                     /locus_tag="VGC69_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0294474.1"
                     /translation="MTASTEQPGMKTHQAPTPIRVGVEMGGTFTDLVWVRPDGTIRTL
                     KVPSTPGAVHEAILAAVAEAGIDLSQVRRFAHGSTVATNALLTRQGAGVGLITTQGFR
                     DVIQIGTHDRVGDLYNMKYRKPLSPVARRHIHEVPERMSAKGGVLHPLDVDAAVATVE
                     TMVQDGIRSIAICLLNSHTNPAHEQQLAGLIRQRHPGVNLSLSSEISPEIREYERCLT
                     TVVNAFVGPMVHDYIGQLDHRLSAGGYDGPLAIMQSNGGIMSADSAGRHAARMLLSGP
                     AAGVRGALHFAQRNGFQDIITLDMGGTSTDVSIARGLQPALMPHIKIDDLTIRIPSLD
                     IVTVGAGGGSIAHRDSAGLLDVGPASAGAYPGPACYGRGGLLPTVSDAQVVAGWLRPE
                     RQANAHFHIRADLANQALRTLDLPGSADSAADGVLRIVNNNMVSAVQLVSTARGIDPA
                     RFVLVAYGGGGPLHGALVAQALGMRKVLIPWSPGVTSALGLLVCDHIVDLAFTQAHAL
                     DASTLGRERLADLRAKAGELAREHALDAPRLEIGLDLHYRGQGYEMTLWLPDALASKE
                     AIQEGFNALHRERYGYCRDAVPVHVLNYRIKLAQPNTSQIPIQAADAESTAQGGLSRR
                     PIRVSGQTCMAVFLPRHGLAIGQVVEGPAIIEESTSTTLVPPGWAARATENGDLLLER
                     NART"
     gene            complement(10536..11324)
                     /locus_tag="VGC69_03965"
     CDS             complement(10536..11324)
                     /locus_tag="VGC69_03965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294475.1"
                     /translation="MHSLSLRLRITVGLIYLFLLLPLAIIALSSFSGSSRFDLFPAEG
                     WSLRWYRSFFHSSNYLTAMFANSLPVALIAGLLATAIGTLAAVAVIRSPEKLRARFEI
                     LTMLPLIVPTVLLGIGLYLLSTILHIKNGFLMLVLGHGLIGIPYVIRIVMAGLEEIDP
                     ALEQAARSLGCSPVQAFIMTTVPQIKTSLASAMIFAFIESFSDINLALFIAGSGNTTL
                     PVQIFSDIVWQGDPTIAAASTLQMVLICLLVMLGFKAVGRRNKP"
     gene            complement(11317..12174)
                     /locus_tag="VGC69_03970"
     CDS             complement(11317..12174)
                     /locus_tag="VGC69_03970"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294476.1"
                     /translation="MRLRSPAWLIAPYGAALAILLLVPICVLVGVSFATYAPSRLWDP
                     IFTLGNYGRLADGFYADMLWRTLRIAASATLITAVVAYPLAYFLARCEAWFAPVGIFM
                     LVVPLMVSTVIRSFGWIVLLGRDGPLIKLLGLLGVTVHGDFLYSELAVQLGLIQILLP
                     LMTLPLLAAIERIPLRAEEAAASLGASPLRVFFMVIVPLSKPGLASGAVLCFTVSLSV
                     VITPELLGGRKVRMAGNFIYEQVLSAFNWPFAASIANILAVISMACMILLIAHLRRRH
                     ASQNGGAHA"
     gene            complement(12180..13256)
                     /locus_tag="VGC69_03975"
     CDS             complement(12180..13256)
                     /locus_tag="VGC69_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294477.1"
                     /translation="MASIQVQELCRDYGPSSKVGPISLDIKSGEFFSLLGPSGCGKST
                     TLRCIAGFEQPDQGEILIAGKSVRRLPAHKRNIGIVFQSFALFPHLTVLGNVMFGLRL
                     RKIPGAQAKARARAALISVGLDDYGDRYPAALSGGQQQRVAIARAIVLEPPVLLMDEP
                     LSSLDLQLRVQMRGEIRALQRRLGLTVVYVTHDQDEALSMSDRLAVMSQGAVQQIGAP
                     REIYEQPASEAVATFIGTANIFTLSALGEDDGGTFGTLPSGLSIRLGRRAFAHPPRLA
                     LRPERICIEPDAQGGGDNLHTGTIERLDYLGGDLALNIRLSDKDVVAVSAKNIGHLAK
                     ARIGDRLRIGFSSHDLVPLPVPGN"
     gene            complement(13288..14298)
                     /locus_tag="VGC69_03980"
     CDS             complement(13288..14298)
                     /locus_tag="VGC69_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0294478.1"
                     /translation="MKLTLASMGAYALGPGLAPAAQAADAPFVWAGVGGRWAEMVVEA
                     FIKGTTFEGKVSQSAQVETIATSKILAACGNAPFAVSNNGPAEAALMAQGKCLLPYDK
                     SNIALLDDLVPAARSDDYFGAFCILGFGLLWNTKEAKEPASFEDMWKPEYKGRIAIPA
                     YGWYGQYWLHAMNKVLGGNENDVSPAIKMISKLVREHDAVLMENNDHAVRLIQSGQVI
                     MAPYWNGSTARLKADGTPVEFKYVPGTLAFGSGFVIPASSSNPDQAQAFIANTLDPVR
                     QQQFSIISRYPPASTKAVLPPEHQSVALPPEAAKNFAQLDWGLVNGTRDKNLELWNRQ
                     VL"
     gene            complement(14371..15093)
                     /locus_tag="VGC69_03985"
     CDS             complement(14371..15093)
                     /locus_tag="VGC69_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0294479.1"
                     /translation="MASKAFLYDRIAAELRHRIETGVYPPLQKLPSIDELVGEFKSSN
                     ITVRRALNELIAKGLIISRQGQGNFVRDSRKIVRHVQPGHMLLNDLSEDIRKAGLEPG
                     VRQLELTLQSPTVEQAEKLRLSRGEKVYRMTSLVLADNIPIAYDHCYIPQAMAEGFGD
                     RLQSTFVVTLLREAGYRIRHMELFIEVIAAEEPELTHLDVPKSFPLLGIRYTPISATG
                     HALITGHFTSRGDRLAYSIDDN"
     gene            complement(15312..16517)
                     /locus_tag="VGC69_03990"
     CDS             complement(15312..16517)
                     /locus_tag="VGC69_03990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEY0294480.1"
                     /translation="MSTLFASVEQAPRDPILGLNEQYNTDTRPGKVNLGVGVYYDDEG
                     RIPLLAAVRKAEVARIEAAAARGYLPIEGIAGYNKGAQTLLLGADSPLIAAGRVLTAQ
                     ALGGTGALKIGADFLKQILPDSKVLISDPSWENHRALFERAGFPVETYAYYDAATRGL
                     NFEGMLAALKSAPAKTIVVLHACCHNPTGVDLDAAQWKQVAEIVKARDLVPFLDIAYQ
                     GFGAGLHEDAEAVRIFAAQDITMLVSSSFSKSFSLYGERVGALTLVAGDKEEAGRVLS
                     QLKRVIRTNYSNPPTHGGTIVSSVLNTPELFALWEQELAGMRDRIRLMRQQLAEKVKA
                     EDSKQDFGFIVKQRGMFSYTGLTSAQVDVLREQYGIYAVSSGRICVAALNSHNIDGVA
                     KAIAAVLKD"
     gene            16676..18769
                     /gene="uvrB"
                     /locus_tag="VGC69_03995"
     CDS             16676..18769
                     /gene="uvrB"
                     /locus_tag="VGC69_03995"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY0294481.1"
                     /translation="MTDDSLIADPIDPGPAGFSDAAASEPRYVDYPGSPFHLFQPYPP
                     AGDQPTAIEGLIQGVEDGLMYQTLLGVTGSGKTFTMANVIARLGRPALVLAPNKTLAA
                     QLYAEMREFFPKNAVEYFVSYYDYYQPEAYVPTRDLFIEKDSSINEHIEQMRLSATKS
                     LLERRDTIIVGTVSCIYGIGNPGDYHAMVLILRAGDRIARREILARLVAMQYTRNDAD
                     FTRGVFRVRGETIDIFPAESPELALRLTLFDDEIESLELFDPLTGRVRQKLPRFTVYP
                     GSHYVTPRDTVLRAIGTIKEELRERSSFLVNDGKLVEAQRLEQRTRFDIEMLQELGFC
                     KGIENYSRHLSGAAPGDPPPTLIDYLPPDALMFIDESHVTIGQLGGMYRGDRSRKETL
                     VQYGFRLPSALDNRPLKLEEFESRMRQCVFVSATPAAYEQQHADNVVEQVVRPTGLVD
                     PEVEVRPARTQVDDLLGQIKSRVGQGDRILVTTLTKRMAEDLTDFLSEHGVRVRYLHS
                     DIDTVERVEIIRDLRLGVFDVLVGINLLREGLDIPEVSLVAILDSDKEGFLRSERSLI
                     QTIGRAARHINGHAILYADHITDSMRRAMGETERRRAKQVQHNLDHGITARGVNKAVR
                     ELIDGVVAPVSHDTLEEAIPAELLSDEKALAREIRRLEKLMMDHARNLEFEQAAATRD
                     ALNALKKRVLLNGAV"
     gene            18996..19487
                     /locus_tag="VGC69_04000"
     CDS             18996..19487
                     /locus_tag="VGC69_04000"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY0294482.1"
                     /translation="MMTKVLFVCMGNICRSPSAVGVFRHLVNDAGLADVVDIDSAGTH
                     AYHIGEAPDARALAAARKRGYDITHCEARQVTADDFREFDLILAMDWDNLSALQQQCP
                     KAYQHKLMLLMRFANEFEEATVPDPYYGGPEGFGKVLDYLEDACQGVLELVRKRATQY
                     QAA"
     gene            19687..20223
                     /gene="iscR"
                     /locus_tag="VGC69_04005"
     CDS             19687..20223
                     /gene="iscR"
                     /locus_tag="VGC69_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEY0294483.1"
                     /translation="MRLTTKGRFAVTAMIDLAMRQHSGPVTLAAISQRQNISLSYLEQ
                     LFGKLRRHELVDSVRGPGGGYSLARLARNVTVADIIFAVDEPLDATSCGGKRDCTSGL
                     DGLNGKCMTHELWATLNQKMVDYLDSVSLQDLVDQQRVRQLQHAANQAQGAAVPMTRS
                     AGNAAGNESPALEVNTAL"
     gene            20245..21456
                     /locus_tag="VGC69_04010"
     CDS             20245..21456
                     /locus_tag="VGC69_04010"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEY0294484.1"
                     /translation="MSNLPIYLDNSATTPVDPRVVDKMIPWLYEHFGNPASRSHAFGW
                     EAEEAVENARAEVAKLVNADPREIVWTSGATESDNLAIKGAANFYAERGKHIITVKTE
                     HKAVLDTCRELERQGFDVTYLDVQEDGLVNLDAFKAALRPDTILVSVMLVNNEIGVVQ
                     DIETLGEICREKGIIFHVDAAQATGKVAIDLQKLKVDLMSFSAHKTYGPKGIGALYVR
                     RKPRVRIEAQMHGGGHERGFRSGTLATHQIVGMGEAFRLAREEMNTENERIRMLRDRL
                     YKGLTQIEETYVNGSMEQRVPHNLNISFNFVEGESLIMAIKELAVSSGSACTSASLEP
                     SYVLRALGRNDELAHSSIRFTIGRFTTEQEVDFAIELIKSRVGKLRDMSPLWEMAKEG
                     IDLNSVQWAQH"
BASE COUNT         4003 a         6709 c         7050 g         3726 t
ORIGIN      
        1 gttcctggaa tcggaaaacc ccgacaagga catctccttc tacatcaact cgcccggcgg
       61 gtcggtgtat gcgggcatgg ccatctatga caccatgcag ttcgtcaaac ccgacgtgtc
      121 cacgctgtgc accgggctgg cggccagcat gggcgccttc ctgctggccg cgggcaagaa
      181 gggcaagcgc tacacgctgc ccaactcccg catcatgatc caccagccct cgggcggcgc
      241 ccagggccag gccacggaca tccagatcca ggcccgcgag atcctggacc tgcgcgcccg
      301 gctcaaccgc atcctgtccg agagcaccgg ccagccgatc gaccgcatcg aggtggacac
      361 cgagcgcgac aacttcatgt ccgccgaaga tgcggtatct tatgggctcg tggacaagat
      421 gctgacgtcc cgcgcggacg cggctggtgt gtaatcgagg tacgaatatt tatgcctgac
      481 aaaaagggtt cggtagacgc gaaagtgctg cattgctcgt tctgcaacaa gagccagcac
      541 gaagtccgca aactgatcgc ggggccgtcg gtgttcatct gtgatgaatg catcgacctg
      601 tgcaacgaca tcatccgcga ggaggcccag gcctccgcgc gcgcggccat tcgttccgag
      661 ctgcccaccc cgtccgagat caagtccttc ctcgaccaat acgtcattgg tcagacctcg
      721 cccaagcgca tgctggcggt ggcggtctac aaccattaca agcgcattcg ccacggcgag
      781 atcaagggcg acgaagtcga gctctccaag agcaacatca tgctcatcgg cccgacgggc
      841 tcgggcaaga cgcttctggc ccagaccctg gcgcgcatgc tgaacgtgcc cttcgtcatg
      901 gccgacgcca ccacgctcac cgaagcgggc tacgtgggcg aagacgtcga gaacatcatc
      961 cagaagctgc tgcagagctg caactacgat gtcgagaagg cacagcgcgc catcgtctac
     1021 atcgacgaga tcgacaagat ctcgcgcaag tccgacaacc cgtccattac ccgcgatgtc
     1081 tcgggcgaag gcgtgcagca ggccctgctc aagctgatcg aaggcacggt ggcctcggtg
     1141 cctccgcagg gcgggcgcaa gcaccccaac caggacttcg tccaggtcga cacgaccaac
     1201 atcctgttca tcgtgggcgg cgccttcgac ggactggaaa aggtcatccg cgaccgtacc
     1261 gaaaagtccg gcatcggttt ctcggcctcg gtgcgcgcca agtccgaacg cggcgtgggc
     1321 gagctgtttt ccgaagtcga gcccgaagac ctcatcaagt tcggcctcat tcccgaactg
     1381 gtcggccgcc tgcccgtggt ggccacgctc gacgagctcg acgaggccgc gctggtgcag
     1441 atcctcaccg agccgaagaa ctccctggtc aagcagttcc agaagctctt cgccatggaa
     1501 ggcgccgagc tcgatgtgcg cccctcggcg ctcagggcca tcgccaccaa ggcgctcaag
     1561 cgcaagaccg gcgcgcgcgg cctgcgttcc attctcgagg cctcgctgct ggacaccatg
     1621 tacgagctgc cttcgcaggg caacgtcaag cgcgtggtgc tcgacgagag cgccgtggaa
     1681 ggcaagggca agcccctgct catctatgcc gacgccgaag gcgagggcga tcccaaggcc
     1741 agccggggcg aggccgccgc ctgaggacgc gcatgcgatg ggcggggttt tgtccctgcc
     1801 cattgaatgg gtaattgtca ggaacgaccc cgtgcgggcc atgccggaca ggcgctgcat
     1861 cgcgccgaaa aacccgtaac cattacgggt tttttcattt ttatgccttg tcccgcggct
     1921 ggtgcttgaa ttttcggcta ttgttcccac ataccagaca taacctatcg gtccggccgg
     1981 atgtagcgta atccggcggt tgccgccctt atacgcagtc aatcgaggaa tctctatgtc
     2041 tgccaatcag accctgcctg cagaacccat cgacctgccg ctgctgccct tgcgcgacgt
     2101 cgtggtattt ccgcatatgg tcatcccatt gtttgtcggt cgcccgcgtt ccatccgcgc
     2161 gctcgaagtc gccatggagg ccggcaagag catcatgctg gtcgcccaga aatccgccgg
     2221 caaggacgac cccacccctg aagacgtcta tgaaatcggc tgcgtggcca gcatcctgca
     2281 gatgctcaag ctgcccgacg gcacggtcaa ggtgctggtc gaaggcacgc agcgtgcccg
     2341 catcgacagc atcgaagacg tcgaatcgca cttcacttgc ctggtcacgc ccatcgagcc
     2401 cgacctggcc cagagttcgg aaagcgaagc cctgcgccgc gccatcgtgg cccagttcga
     2461 gcagtacgtg aagctcaaca agaagatccc gcccgagatc ctcacctcgc tggccggcat
     2521 cgacgatccg ggccgcctgg ccgacaccat cgccgcgcac ctgccgctca agctcgagca
     2581 gaagcaggcc atgctcgaga tcaccggcac gaccgagcgc ctcgaaggcc tgctgacgca
     2641 gctcgagacc gagatcgaca ttctccaggt cgagaagcgc attcgcggcc gcgtcaagaa
     2701 gcagatggag aagagccagc gcgactacta cctgaacgaa caggtcaagg ccatccagaa
     2761 ggaactgggc gaaggcgaag agggtgccga tctcgaagaa ctcgagaaga agatcatcgc
     2821 cgcgcacatg cccaaggaag cgcgcaagaa ggccgatgcc gagctcaaga agctcaagct
     2881 catgtcgccc atgtcggccg aagccacggt ggtgcgcaac tacatcgaca cagtcatcaa
     2941 tcttccctgg aagaagaaga gcaaggtcaa caattccgtc agccatgccg agacggtgct
     3001 cgacaacgac cactacggcc tcgagaaggt caaggaacgc atcgtcgagt atctggccgt
     3061 gcagcagcgc gtcgacaagc tcaaggcgcc catcctgtgc ctggtcgggc ccccgggcgt
     3121 gggcaagacc tcgctggggc agtccatcgc ccgcgccacg aaccgcaagt tcgtgcgcat
     3181 ggccctgggc ggcgtgcgcg acgaggccga gatccgcggc caccgccgca cctatatcgg
     3241 ctccatgccg ggcaagatcc tgcagaacct gtccaagacc ggcacgcgca atccgctctt
     3301 cctgctcgat gaaatcgaca agctgggcat ggatttccgc ggcgatccgg cctcggccct
     3361 gctggaagtg ctcgaccccg agcagaacca caccttccag gaccactacg tcgaggtcga
     3421 cttcgacctg tcggatgtga tgttcgtcgc caccagcaac acgctgaaca tcccgccggc
     3481 gctgctcgac cgcatggaag tgatccgcct gtcgggctac accgaggaag agaagatcca
     3541 catcatccgt gaccatctgc tgcccaagct gatgaagaac aacggcgtga aggaaaacga
     3601 actcgaggtc gacgactccg cactgcgcga catcgtgcgc tactacacgc gtgaagccgg
     3661 cgtgcgctcg ctcgagcgcg aagtcggcaa ggtgtgccgc aaggtggtca agaagctgct
     3721 cgtccagcag gccagcaacg ccaaggacgg cgagaatgcc gagcccctga tcctgcccgt
     3781 caaggtcacg agcgaaaacc tcagcgacta cctgggcgtg cgccgctata ccttcggcat
     3841 ggccgagaag gaaaaccaga ttggccaggt gaccggcctt gcctggacgg aagtcggcgg
     3901 cgaactgctc accatcgaag tggtcgacat ggcgggcaag ggcgcgatcc agcgctcggg
     3961 ttcgctgggc gatgtcatga aggagtccgt cgaggtcgcg cgcacggtgg tgcgttcgcg
     4021 cgctcgccgc ctgggctttg ccgacagcgt gttcgaaaag cgcgacatgc acatccactt
     4081 tcccgaaggc gccacgccca aggatggtcc gtccgccggt atcgccatca ccacggccat
     4141 ggtgtccgcg ctgtcgcgca ttcccgtgcg tgccgacgtg gccatgacgg gcgagatcac
     4201 gctgcgcggc gaggtcctgc ccatcggcgg cctgaaggaa aagctgctgg cggcgcatcg
     4261 tggcggcatc aagacggtgc tgatcccgga agagaacgtc aaggacttgg ccgagatccc
     4321 cgacaacgtc aagaactcgc tcgagatcgt gccggtgcgc tggatcgaca aggtgctgga
     4381 gctggccctg gaacgcctgc ccgagcccct ggccgaggaa gacgcggcca aaaccgacgt
     4441 cgtggcaacc caggctgcgc cggcggtgga cccggtggtc aagcactgac cggtgtgcgc
     4501 atgaacaagg cccacgacgt tgtcgtgggc cttgttgttt gtgtcgggac ctgccaaagg
     4561 caggtgaagt tacatgcagc ctttcgcaat ctgcgagagg acgccggcgg cagtggtaac
     4621 ggctaccgcg gcaggacagt ctagaaaaaa gcagtctcca gtgacagagg gagtgagtgt
     4681 ttctgccacg ccagtcgttg cctgtggatt caatgcaagg cttgcgtgcg gatcagcccg
     4741 accgcgatgc cttccagggc gaagttctgc ccgggctgga ccacgatggg ctcgaagtcg
     4801 gggttctcgg gcagcagctc gatacggccg ctctggcgct gcaggcgctt gacggtgacg
     4861 tcgtcgtcga tgcgggcgac cacgatctgg ccgttgcggg cttcggcggc acgcttgacg
     4921 gccaagaggt cgccttcgag gatgccggca tcgcgcatgc tcatgccgcg caccttgagc
     4981 agatagtcgg gcgtttgcga gaagagggcc gggtcgacgc cgatttcgcg ctcgacatgt
     5041 tcggcggcca ggatggggct gcctgccgcc acacggccca ccaggggcaa cagcaggcgc
     5101 gagaaggcgg cggccgaatc ggcgatcagg gcgccggcac tggcgagcgc cgaggcggcc
     5161 agccccgcgt cggtgcggat gtcaccctct ccgaccagca ggcggatgcc gcgcgaggcg
     5221 ccggccgtga gctcgatggc tcccttgcgg gccagggcct tgagatggtc ttccgccgca
     5281 ttgggcgagc gaaaccccag cgcctgggcg atttcagccc gggtaggcgg aaatcccgtg
     5341 tgcgccacgg cggcgcggat gagatcgagg atttcctgct ggcgtgcggt gagtttgctg
     5401 gccatggcga tgatttggaa tgcaaattta agctgtatat ttatacagca attttgctgg
     5461 gccaggagac aaatagcaag gattttgtcg cttttgcaca gggcggcggc aggcagctat
     5521 ctgccggcga ggtccatgag ccagttccat ttcatgacgg cgttgagcac gggcgcccgc
     5581 aggccatgca gggcaatggg ccggatcggc gtcgccggca ccccggtgtc gcccgtcttg
     5641 cccaggatgg tgtccgacag ccaggcgccg gcgcgtgtcg ccagcgccac gccgcgccca
     5701 ttcatgccga ccagcacatg catgccatcg gccggccgat ggtaatgcgg caggaagtcc
     5761 ggtgtgaggc agaccaggcc gcgccagcgg tattcgaccg gcagctcgcg ggtttgcgga
     5821 tagatgccgt gcaatacgcg ttccagcacg tgatagtcgc tggcctgttc tccggagccg
     5881 gtttgggagg agaacgaggc gcggccgccg agcatcaggc ggccatcagg ggatttccgg
     5941 aaaaagagaa tcagccggcg gctgtcgtat acggcttggc ctccaggcaa aatgctgtct
     6001 tgcaggtggg cgggcagcac gccggtagcg atctggaagc aattcacggg cagcagcgtg
     6061 cgggccaggc cgggcactag ctggtcggta tagccgttgg tggcgacgac aacgccatgg
     6121 gcgcgaaccg agcccgtggc ggtacgcagt tcccattgct tgccgtgccg gacggcggcg
     6181 aggacgggcg agttgcaatg aatcgccgcg ccgctgtcgg cagcggcacg ggccaggccg
     6241 cgtgccagcg ccaacgcatg aacagaaccg ccgcgcatgt cgagcagggc gcctgcgtag
     6301 gcggtggtgc cggtgtgggc ctcggtctgt gcggcatcga gccggcgcgc gtcaatgccg
     6361 cgggcacgca ggtcggcgca agccgcatcc aggtttttca gcgcgcggac attgtgcgcg
     6421 caacgcagca caccggggaa actcaggttg cagtccaggt tcttgcggcg caccagctcg
     6481 gccaggaagg ccggcccttc ttcgcccagc cggaaaaacc cgtcgccagc cgggccgaag
     6541 cgatgcacca attcttcacg gccccatttc agcccggggt tgacctgtcc accgttgcgg
     6601 ccgctggcgc cgaagccgat atccccggct tccagtaaaa cgaccttgcg gcctgattcg
     6661 gccaggtgca tggccgtgga caagccgagg tagcccgctc cgacgacggc cacgtccgcc
     6721 tgcgccgatc cgtccagcgg tggcgaggcg ccgtcgggca ggccggcggt ggcgcgccac
     6781 aagttgccgg catgcgccgg ttcgcccggc gggcgatcgt gccatgtcat gccgacacgc
     6841 cagggggatg cgtgacccat ccgtcaacca ggtcggtctg gatggcttcg gacgcgcgcc
     6901 gcgccgggtc gccccagccg ccgccgccgc ccactcggat tgtcaaccgg tcgccacgtt
     6961 tcagcggaaa ggtggacatc gcatcgagcc ggattttttc gccatcgcgc aggacttcca
     7021 gcgctcccag gccgcctgga gttccgccat gcaggccctg ggccgcgaat cgaaaacgat
     7081 cggaatagat gctgacatcc gcgccgtctt ccaggatgtc gaacgtgcgc tggaatccca
     7141 ggccgcctcg ccactggccg gcgccgccgc tgtcgggctc gatggcgaaa gaggccacgc
     7201 gcaaatgatc atgcgcgtgc tcgatgactt cgatcggggt gcagcggcag ttgctcatga
     7261 tggcatcgac ggcatcggcg ccgtcgtatc cttgcccggc gcccagcccg ccgccgaaga
     7321 tgtcgccgta tatccgggtg ctgccgtcct ggcgctgctg tgaaatatag acggcggttg
     7381 tcgtattgaa gcccggcgcg gtggcttgtt ccggcatggc gccggccagc gcgaccaaga
     7441 tggcgtcaaa ggcccgatac gcgacaatgg cccgggcgcg caccggggcg ggccagtgcg
     7501 gattgaggat cgagccttca gggaaccggg acacgatcgg cctgttgcag ccatcattcg
     7561 atggcagttt ctggtcggag accaggcagc gtatggccga caaggcggcg gcgcgcgacg
     7621 aggccggcgg gcagttgaac atgctggcta cttgcgcgtg cgtgccggca tagtcgagtt
     7681 cgatatcgct gccgctgacg gtcactttta ccttgacctc tatgggtatg gggtcgaaga
     7741 cgtcgcggtc catgcgcgcg ctgccgtgat agaccccgtc cggtatgcgg gcgagggcct
     7801 ggcgcatgcg cgtctcgcca tgatcgagaa tggcgtccat ggcttggcgt acggtgtcgg
     7861 gcccttcgcg ccgggccagg gccgacaggc gttcgatgcc aatgtgattg gctgcgaatt
     7921 gtgcattgag gtccccgcgg agaatatcgg gcaggcggac attggcgcag atcagcgatt
     7981 caagattgcc atgctcccaa tcgtttcgaa tattgaagat catcggagaa atgacgatgc
     8041 cttcttgaag caagtgggtg gcgcgcacgt tgatgccggc atcggctccg cctatgtcga
     8101 tgtggtgggc ggtgcttccc acatagccta gcaatgcgcc gtcgaacagg acgggctgga
     8161 acagaatcaa gtcattcaaa tgctggccgc ccgcataagg gtcgttcatc agcatggcgt
     8221 ggccatcggt caggcggccc aggtctaccc gttgcgaggc cccgtggaat gcttcggaca
     8281 aggcgacggt ttgcatggga atcaaggccg attgcgccac gacttcgcca tcgggcgtga
     8341 gaatggcggt ggagcagtcg cgcgcttccc gtatgaccgt ggagaaagag gagcgaacca
     8401 ggttcgcgcc catttcttcc gtgatggagc gtagcgcctg gttcaggatc gaaagctgta
     8461 tggggtccag ggaagaagtc atgtccttgc gttcctttcc agcagcaggt cgccattttc
     8521 cgtcgcccgg gctgcccagc ccgggggcac cagggtcgtg gatgtggatt cttcaatgat
     8581 ggccggtccc tccacgactt gaccaatggc caagccatgg cgcggcagga aaacagccat
     8641 gcaggtttgg ccggataccc ggatggggcg gcgtgacagc ccgccctgcg cggttgattc
     8701 ggcatctgct gcctggatgg gtatctggct ggtgttcggc tgcgccagtt tgattcgata
     8761 gttcaataca tgcacgggta cggcgtcgcg acagtagccg tatctctccc ggtgcagggc
     8821 attgaaacct tcctggatgg cttctttcga ggcgagggcg tcgggcagcc acagcgtcat
     8881 ctcgtatccc tggccccggt aatgcaaatc caggccgatt tccagcctgg gcgcgtcgag
     8941 cgcgtgctcg cgggccagtt ccccggcctt ggcccgcaga tccgcgagcc gctcgcgccc
     9001 cagggtggag gcatccagcg catgggcctg cgtgaaggcc aggtcgacga tatggtcgca
     9061 aaccagcagc cccagggcgc tcgtcacgcc aggcgaccag ggaatgagaa ccttgcgcat
     9121 gcccagcgct tgcgcgacca gcgcgccatg caaggggccg ccgccgccat aggcgaccag
     9181 gacgaatcgg gccgggtcta tgccgcgcgc cgtggaaacc agctgaacgg cggacaccat
     9241 attgttgttg acgatgcgca gcacgccatc ggcggcgcta tcggccgagc cgggcagatc
     9301 cagggtgcgc agcgcctggt tcgcgaggtc ggcgcggatg tggaaatgcg cattcgcctg
     9361 ccgctccggt cgcagccagc ccgcgacgac ctgcgcgtcg gatacggtgg gaagaaggcc
     9421 gcctcggccg tagcaggcgg gacccggata ggcgcccgcg ctcgcggggc cgacgtccag
     9481 cagtcccgcg ctgtcgcggt gcgcaatact gcctcccccg gcgcccaccg tcacgatatc
     9541 gagggaagga atgcggatgg tcaggtcgtc tattttgatg tgcggcatca gcgcgggttg
     9601 cagcccgcgc gcgatggaca catcggtgct ggtgccgccc atgtcgagcg tgatgatgtc
     9661 ctggaagccg ttgcgctgcg cgaaatggag ggcgccccgc actcccgccg ccggcccgga
     9721 aagcagcatg cgcgcggcat gccggcccgc gctgtcggcg gacatgatgc cgccgttgga
     9781 ttgcatgatc gccagggggc cgtcgtagcc gcccgcgcta aggcgatggt caagctgccc
     9841 gatgtaatcg tgcaccatgg ggcccacgaa ggcgttcaca accgtcgtca gacaacgttc
     9901 gtactcgcgg atctccggcg aaatttccga gctgaggctg agattgacac cgggatgccg
     9961 ctgccggatc aagccggcca actgctgctc gtgggcggga ttcgtatggc tgttcagcag
    10021 gcatattgca atcgaacgga tgccatcttg caccatggtc tccaccgtgg cgacggctgc
    10081 gtcgacatcc agggggtgca gcacgccgcc tttggcgctc atgcgttccg gcacttcgtg
    10141 gatatgccgg cgcgccaccg gcgatagggg tttgcggtac ttcatgttgt acaagtcgcc
    10201 gacccggtcg tgggtgccga tctggatgac gtcccggaat ccctgtgtcg tgatcaggcc
    10261 gacaccggcg ccctggcggg tgagcagggc attggtggcc acggtcgagc catgggcgaa
    10321 gcgccgtacc tggctcaggt cgattcccgc ttccgctacc gcggccagga tcgcctcgtg
    10381 gacggccccg ggcgtggacg gcactttcag ggttcggatg gttccatcgg gccggaccca
    10441 aaccaggtcg gtgaaagtcc cccccatctc gacgccgacg cgaatggggg tcggcgcctg
    10501 gtgcgttttc atgccgggtt gttcggtgct tgcggtcatg gcttgttcct gcgtcctact
    10561 gccttgaagc caagcatcac gagcaggcag atcaggacca tctgcaaggt cgacgcggcg
    10621 gcgatggtcg ggtctccctg ccagacgatg tcggagaaga tctggacggg gagcgtggtg
    10681 ttgccggaac cggcgatgaa cagcgccaga ttgatatcgc tgaaggactc gatgaaggcg
    10741 aagatcatgg ccgaggccag gctcgtcttg atctggggca ccgtggtcat gatgaaggcc
    10801 tggacggggc tgcaacccag gctccgggct gcctgctcca gcgccgggtc gatttcttcc
    10861 aggccagcca tgacgatgcg gatcacataa gggatgccga tcaggccatg gcccaggaca
    10921 agcatgagaa agccattctt gatgtgcagg atcgtgctca gcaaatacag gccgatgccc
    10981 agcaggaccg tgggaacgat cagcggcagc atggtgagta tctcgaacct ggcccgcagc
    11041 ttttccgggc tgcgaatcac cgcaacggcc gccagcgtgc cgattgccgt cgccagaagg
    11101 cctgcgatca acgccaccgg caggctgttg gcgaacatgg cggtcaggta gttgctgcta
    11161 tggaaaaaac tccgatacca gcgcagcgac cagccttccg ccgggaacag gtcgaagcgg
    11221 gatgagccgc tgaaagaact gagcgcgatg atggccaggg gcagcagcag gaaaagatag
    11281 atcagcccca cggtaatgcg cagtcgaagc gaaaggctat gcatgggcgc ctccgttttg
    11341 cgatgcgtgt ctgcggcgca gatgggcgat gagaagaatc atgcaggcca tggagatcac
    11401 ggccaggatg ttggcgatgc tggcggcgaa tggccagttg aaggcgctca gcacctgttc
    11461 gtaaatgaaa ttgccggcca tgcggacttt tctgccgcct agcagttcgg gtgtgatgac
    11521 cacgctcagg ctgacggtaa agcacagtac cgcgccggaa gccaggccgg gcttgctgag
    11581 cggaacgatg accatgaaga aaaccctcag cggagaggcg cccaggctgg cggcggcctc
    11641 ttcggcgcgc aggggaatcc gttcgatggc ggccagcagc ggcagggtca tcagcggcaa
    11701 gagaatctga atgagcccca gttgcacggc cagctccgaa tacagaaaat cgccgtgcac
    11761 ggtcacgccc aggagtccca gcagctttat caaagggccg tcccggccca gcagcacgat
    11821 ccagccaaat gaacggatca ccgtgctgac catgagcggc accaccagca tgaaaatccc
    11881 gaccggtgcg aaccaggctt cgcagcgcgc cagaaaatag gcaagcggat aggcgacgac
    11941 cgccgtgatc agcgtggccg aggcggcgat gcgcagcgtc cgccacagca tgtcggcgta
    12001 gaagccgtca gccaggcggc catagttgcc gagggtgaag ataggatccc acaagcgcga
    12061 cggggcatag gtggcaaagc tgacgccgac cagcacgcag atcggcacca gcagcaggat
    12121 ggccaaggcc gcgccatagg gagcgatcag ccatgcgggg gagcgcaatc gcatgatggt
    12181 caattccccg ggacgggtag cggcacgaga tcgtgggacg agaagccgat gcgtaggcgg
    12241 tcgccgatcc tggctttggc gagatggccg atattcttgg ccgagaccgc cacgacatcc
    12301 ttgtctgaca agcggatgtt cagggcaagg tcgccgccca gatagtcgag gcgctcgatc
    12361 gtcccggtgt gcaggttgtc gccgccgccc tgggcatcgg gctcgatgca tatgcgttcc
    12421 ggcctgagtg cgagccgggg cggatgggcg aaagcgcgcc ggcccaggcg gatggacagg
    12481 ccgctgggca gggtgccgaa tgtgccgccg tcgtcttccc ccagcgccga caacgtgaat
    12541 atattggccg tgccgatgaa ggtcgcgacc gcctcgctgg cgggctgctc gtaaatctcc
    12601 cgcggggcgc cgatttgctg cacggcgcct tgcgacatca cggcgagcct gtccgacatg
    12661 gacagcgctt catcttgatc gtgcgtgaca tacaccacgg tcaagccgag gcggcgttgc
    12721 aatgcccgga tctcgccgcg catctgcacc cgcagctgca gatccaggct tgaaagcggc
    12781 tcgtccatca gcaggaccgg gggctccagg acgatcgccc gggcgatggc cacacgctgc
    12841 tgctggcctc ccgacagcgc ggccggatag cggtcgccat aatcatccag gccgactgaa
    12901 atcaaggcgg cccgagcgcg ggccttggcc tgcgcgccgg ggatcttgcg caggcgcagg
    12961 ccgaacatga cgttgcccag gacggtcaag tgagggaaca gggcaaaact ttggaagacg
    13021 atgcctatat tgcgcttgtg cgccggcaag cgtcgaacgg acttgccggc aatcaggatt
    13081 tctccctggt ccggttgttc aaaccccgcg atgcagcgca gcgtcgtcga ttttccgcaa
    13141 ccgctgggcc cgagcagcga gaagaactcc cccgacttga tgtcgagcga gatggggccg
    13201 accttgctgc ttgggccgta atcgcggcac agctcctgga cctgtatcga ggccatcggt
    13261 catgctcctg ctgatgaaat atccatgtca aagaacctgc cggttccaga gttcgagatt
    13321 cttgtcccgg gtgccgttga ccaggcccca atcgagctgg gcgaagttct tggcggcttc
    13381 gggcggcagg gcgaccgatt gatgctcggg cggcagtacc gccttggtgc tggcgggcgg
    13441 atagcggctg atgatggaga actgctgctg gcgtaccgga tccagggtgt tggcaatgaa
    13501 tgcttgcgcc tggtcggggt tgctgctcga ggcgggaatg acaaagccgg agccgaaggc
    13561 cagcgtgccg ggcacgtact tgaattcgac aggcgtgccg tcggccttca ggcgcgcggt
    13621 ggagccgttc caataggggg ccatgatgac ctggccagac tggatcagcc gtaccgcatg
    13681 gtcattgttt tccatcaata ccgcatcatg ttcgcggacg agcttggaga tcatcttgat
    13741 cgcgggtgag acatcgtttt cattgcctcc cagcaccttg ttcatggcgt gcagccaata
    13801 ctggccgtac cagccgtagg ccgggatggc gatccggcct ttgtattcgg gcttccacat
    13861 gtcctcgaac gaggccggtt ccttggcttc cttggtgttc cagagcaggc cgaacccgag
    13921 gatgcagaag gcgccgaagt aatcgtcgct gcgcgcggcc gggaccaagt cgtcgaggag
    13981 agcgatattg gacttgtcgt aaggcagcag gcatttgcct tgcgccatca acgcggcttc
    14041 ggcggggccg ttattgctga ccgcgaacgg cgcgttgccg caggcggcga ggattttcga
    14101 tgtggcgatg gtttccactt gggccgactg gctgaccttg ccttcgaacg tggtgccttt
    14161 gatgaaggcc tccaccacca tttcggccca tcttccgccc acgcctgccc agacaaacgg
    14221 cgcgtcggcg gcctgcgcgg cgggggccag gccgggaccc agcgcatagg cgcccatgct
    14281 ggctaacgtc aacttcaata catctcgtct tgtggtcatg gcattcccct tgatatgtga
    14341 ttgaaatgaa tcgacgcaat ggcaggcttg ctagttgtcg tcgatgctgt aggcgaggcg
    14401 gtcgccacgc gaagtgaaat ggcccgtgat cagggcatga cccgtggccg aaatgggggt
    14461 gtagcggatc cccagcaggg ggaagctctt gggcacgtcg agatgggtga gttcgggctc
    14521 ttcggccgcg atgacctcga tgaagagttc catgtggcgt atccgatagc ccgcttcgcg
    14581 cagcagcgtc accacgaagg tcgattgcaa tctatcgccg aaaccctccg ccatggcctg
    14641 cgggatatag cagtggtcgt aggcgatggg aatgttgtcg gccagcacga ggctggtcat
    14701 ccggtagact ttctcgccgc gtgacaggcg tagtttttcc gcttgttcaa ccgtggggga
    14761 ttgcagggtc agttcgagct ggcgcacgcc gggctccagc ccggctttgc ggatgtcttc
    14821 ggacaaatcg ttgagcagca tgtgcccggg ttgcacatgg cggacgattt tgcggctgtc
    14881 gcgcacgaag ttgccttgcc cctggcgtga aatgatcaag cccttggcga tcagttcgtt
    14941 caacgcgcgc cgcacggtga tgttgctcga cttgaactcg cccaccagct catcgatgct
    15001 gggcagcttt tgcagcggcg gatacacgcc ggtttctatg cggtgccgta gttccgccgc
    15061 gatgcgatcg tagagaaatg ccttgcttgc catggaggga ggacgctttt cttaatattc
    15121 aactctgtat acagagtata ttgtttgaaa gtacgggaca gggcaatagc ggcttgccct
    15181 ttcgagggta tttcctagga ggagggccgg cgctgcggcg caggctgaaa gcaaaaaggg
    15241 caaacgcttt ggcgtttgcc cttcgggtcc cggcggtatc gccggatgag gcggggacct
    15301 aaaagaggcg atcagtcctt gagcaccgcc gcaatggcct tggccacgcc atcgatgttg
    15361 tggctgttga gcgcggccac gcagatgcgg ccgctgctga cggcgtagat gccgtattgc
    15421 tcgcgcagca cgtcgacctg ggccgaggtc aggccggtgt acgagaacat gccgcgctgc
    15481 ttcacgatga agccgaagtc ctgcttgctg tcttcggcct tgaccttctc ggccagttgc
    15541 tggcgcatca ggcggatgcg gtcgcgcatg ccggccagtt cctgttccca cagggcgaag
    15601 agctcgggcg tgttgagcac gctggagacg atggtgccgc catgggtggg cgggttggag
    15661 tagttggtgc ggatgacgcg cttgagctgg ctcagcacgc ggccggcttc ttccttgtcg
    15721 ccggccacca gggtcagggc gcccacgcgc tcgccgtaca gggagaacga cttggagaac
    15781 gaggaactca ccagcatggt gatgtcctgc gcggcgaaga tgcgcacggc ttcggcgtct
    15841 tcgtgcaggc cggcgccaaa gccctggtag gcgatgtcca ggaagggcac caggtcgcgc
    15901 gccttgacga tctcggccac ttgcttccac tgggccgcat cgaggtcgac gccggtgggg
    15961 ttgtggcagc aggcgtgcag cacgacgatg gtcttggccg gggccgactt gagggcggcc
    16021 agcatgcctt cgaagttcag gccgcgggta gcggcgtcgt agtaggcgta ggtttcgacc
    16081 gggaagccgg cgcgctcgaa cagggcgcgg tggttttccc agctggggtc gctgatcagc
    16141 accttggagt cgggcaggat ctgcttgagg aagtcggcgc cgatcttcag cgcgccggtg
    16201 ccgcccaggg cctgggcggt gagcacgcgg ccggcggcga tcaacggcga atcggcgccc
    16261 agcagcagcg tctgcgcgcc cttgttgtag ccggcgatgc cctcgatggg caggtagccg
    16321 cgggcggcgg cggcttcgat gcgggccact tcggccttgc gcaccgcggc cagcaggggg
    16381 atacggcctt cgtcgtcgta gtacacgccc acgcccaggt tcactttacc ggggcgggtg
    16441 tcggtgttgt attgctcgtt cagaccgaga atggggtcgc ggggggcttg ttcgacggag
    16501 gcgaagaggg tgctcatgga cgtggttgca tcagtggtcg gacgtggccg cggcgctgct
    16561 tgccagaggg tgtcggggtt gcctggaagg ggtagctgcc gggcgagcca accgggtgga
    16621 cttgctgccg tggcctgtgg ataatggggg gtttggccct tgcctagagt ctagcatgac
    16681 cgacgactcc ttaattgccg atcccatcga tcccggccct gcgggttttt ccgacgccgc
    16741 ggcttccgag ccccggtatg tcgattatcc cggcagcccc ttccacctgt tccagcccta
    16801 tccgccggcg ggcgaccagc ccacggccat cgaggggctg atccagggcg tggaagacgg
    16861 cctcatgtac cagacgctgc tgggcgtgac gggctcgggc aagacgttca ccatggccaa
    16921 cgtgatcgcc cggctgggcc ggccggccct ggtgctggcg cccaacaaga cgctggccgc
    16981 gcagctctac gccgagatgc gcgagttctt cccgaagaac gcggtggagt atttcgtctc
    17041 gtactacgac tactaccagc ccgaggccta cgtccccacg cgcgacctgt tcatcgagaa
    17101 ggactcgtcc atcaacgagc acatcgagca gatgcgcctg tcggccacca agagcctgct
    17161 cgagcgccgc gacaccatca tcgtgggcac ggtgtcgtgc atctacggta tcggcaaccc
    17221 cggcgactac cacgccatgg tgctcatcct gcgcgcgggc gaccgcatcg cgcgccgcga
    17281 gatcctggcg cgcctggtgg ccatgcagta cacccgcaac gatgccgact tcacgcgcgg
    17341 cgtgttccgc gtgcgcggcg aaaccatcga catcttcccg gcggaaagcc cggaactggc
    17401 cctgcgcctg acgctgttcg acgacgagat cgagtcgctc gaactgttcg acccgctgac
    17461 cggccgcgtg cgccaaaagc tgccgcgttt cacggtgtac ccgggttcgc attacgtgac
    17521 cccgcgcgac acggtgctgc gcgccatcgg caccatcaag gaagagttgc gcgagcgctc
    17581 gtcgttcctg gtcaacgacg gcaagctggt cgaggcgcag cgcctggagc agcgcacccg
    17641 cttcgacatc gagatgctgc aggagctggg cttctgcaag ggcatcgaga attactcgcg
    17701 ccacctgtcg ggcgccgcgc cgggcgatcc gccgcccacg ctgatcgact acctgccgcc
    17761 cgacgcgctg atgttcatcg acgaaagcca cgtcaccatc ggccagctgg gcggcatgta
    17821 ccgcggcgac cgttcgcgca aggaaaccct ggtgcagtac ggctttcgcc tgccgtcggc
    17881 gctggacaac cggccgctca agctcgagga gttcgagtcg cgcatgcgcc agtgcgtgtt
    17941 cgtatcggcc acgccggccg cctacgagca gcagcatgcc gacaatgtgg tcgagcaggt
    18001 ggtgcgcccc acggggctgg tcgacccgga ggtggaagtg cggccggccc gcacgcaggt
    18061 cgacgacctg ctgggccaga tcaagtcgcg ggtggggcag ggcgaccgca tcctggtcac
    18121 cacgctcacc aagcgcatgg ccgaggacct taccgacttt ctcagcgaac acggcgtgcg
    18181 ggtgcgctac ctgcactcgg acatcgacac cgtggaacgg gtcgagatca tccgcgacct
    18241 gcgcctgggc gttttcgacg tgctggtggg catcaacctg ctgcgcgagg gcctggacat
    18301 ccccgaggtg tcgctggtgg cgatcctgga ctccgacaag gagggcttcc tgcggtcgga
    18361 gcgcagcctg atccagacca tcggccgggc ggcgcgccac atcaacggcc atgccatcct
    18421 gtatgccgac cacatcaccg actcgatgcg gcgcgccatg ggcgagaccg agcgccgccg
    18481 cgccaagcag gtccagcaca atctggacca cggcatcacg gcgcggggcg tgaacaaggc
    18541 cgtgcgcgaa ctgatcgacg gcgtggtggc gccggtttcc cacgacacgc tggaagaggc
    18601 cattcccgcg gagctgctgt ccgacgaaaa ggccctggcc cgcgaaatcc gccgcctgga
    18661 aaagctgatg atggaccacg cgcgcaacct ggagttcgag caggccgccg ccacccgcga
    18721 tgccttgaac gcgctgaaaa agcgcgtgct gctcaatggg gccgtatagc ggggcggctg
    18781 tcagggcgtg aaagcgcgtg ttgtcgcggg aaagtaattc agctggaata cagatgtgct
    18841 gccttgcaac tcaaatatcc tagatttaaa atgaaaaggt gattccaagc gtttgatttg
    18901 taaatatata aggctttggg acgcggcaag gcgccctggc gtgcatctgc aaaaaaagct
    18961 tgctatattc cgggttttcc ctcacacttt agcccatgat gaccaaagtt cttttcgttt
    19021 gcatgggcaa tatctgccgc tccccgagcg cggtgggtgt gtttcgccat ctggtgaatg
    19081 acgccggctt ggccgacgtc gtggatatcg attccgccgg cacgcacgcc tatcatatcg
    19141 gggaggcgcc tgacgcccgc gccctggccg ctgcccgcaa gcgcggctac gacatcaccc
    19201 actgcgaagc ccgccaggtg acggcggacg atttccgcga gttcgatctc atcctcgcga
    19261 tggactggga caacctttcg gccctgcagc agcagtgccc caaggcttac cagcacaagc
    19321 tcatgctgct gatgcgtttt gccaacgaat tcgaagaagc caccgtgccc gatccgtact
    19381 acggcgggcc cgaaggtttc ggcaaggtgc tcgactacct ggaagacgcc tgccagggcg
    19441 tgctggaact ggtacgcaag cgcgcgaccc aataccaggc cgcctgaaag aagcgggcgc
    19501 ccgcaacaaa aaaccgcgcc tcggcgcggt tttttgttgg tcttcttgaa attgagaatt
    19561 gttctcaact tcgtcgatct agcggccaaa agcgattaac cctgctcgga acgcggattg
    19621 gtctagtgat ttactcggga atttgctata cttgatgcaa ttactcaggt attgaggaaa
    19681 cccatcatgc gtcttactac caaagggcgt ttcgccgtca cggccatgat cgatctggcc
    19741 atgcgccagc acagcggtcc ggtcaccctg gcagccatca gccaacgcca aaatatttcc
    19801 ctgtcgtacc tcgaacagct gttcggcaag ctgcgccgcc acgagctggt cgacagcgtg
    19861 cgcggccccg gcggcggcta ttccctggcc cgcctggccc gcaacgtcac cgtggccgac
    19921 atcatcttcg ccgtcgacga accgctcgac gccaccagct gcggcggcaa gcgcgactgc
    19981 accagcggcc tcgacggcct gaacggcaag tgcatgaccc acgagctgtg ggccacgctc
    20041 aaccaaaaaa tggtcgacta cctcgactcc gtctcgctgc aggatctggt cgatcagcag
    20101 cgcgtgcgcc agctgcagca cgcggccaat caggcccagg gtgcggcagt gcccatgacg
    20161 cgttcggccg gcaatgcggc cggcaacgag tcgccggcgc tcgaggtcaa taccgcccta
    20221 taagctattg gagccacccg catcatgagc aatcttccga tctatctcga caattcggcc
    20281 acgacgccgg tcgacccccg cgtggtcgac aagatgattc cctggttgta cgagcatttt
    20341 ggcaatccgg cctcgcgcag ccacgccttc ggctgggaag ccgaagaggc cgtggaaaac
    20401 gcccgcgccg aggtcgccaa gctggtcaac gccgatccgc gcgaaatcgt ctggacttcc
    20461 ggcgccaccg agtccgacaa cctggccatc aagggcgcgg ccaatttcta cgccgagcgc
    20521 ggcaagcaca tcatcacggt caagaccgag cacaaggcgg tgctggacac ctgccgcgaa
    20581 ctcgagcgcc agggcttcga cgtcacctac cttgacgtgc aggaagacgg cctggtcaac
    20641 ctggacgcct tcaaggcggc gctgcgtccc gacaccatcc tggtgtcggt gatgctggtc
    20701 aacaatgaaa tcggcgtggt gcaggacatc gagaccctcg gcgagatctg ccgcgagaag
    20761 ggcatcatct tccacgtgga cgccgcccag gccaccggca aggtcgccat cgacctgcaa
    20821 aagctcaagg tcgacctgat gtcgttctcg gctcacaaga cctacggccc caagggcatc
    20881 ggcgccctgt acgtgcgccg caagccgcgc gtgcgcatcg aggcgcagat gcatggcggc
    20941 ggccacgagc gcggtttccg ctcgggcacc ctggccacgc accagatcgt cggcatgggc
    21001 gaggccttcc gcctggcccg cgaggaaatg aacaccgaga acgagcgcat ccgcatgctg
    21061 cgcgaccgcc tgtacaaggg cctgacgcag atcgaggaaa cctacgtgaa cggcagcatg
    21121 gaacagcgcg tgccgcacaa cctcaacatc agcttcaact tcgtggaagg cgagtccctg
    21181 atcatggcga tcaaggaact ggccgtctcc agcggctcgg cctgtacctc ggccagcctg
    21241 gagccgtcct acgtgttgcg cgccctgggc cgcaacgacg aactggcgca cagctccatc
    21301 cgcttcacca ttggccgctt cactaccgag caggaagtcg acttcgccat cgagctcatc
    21361 aagagccgcg tcggcaagct gcgcgacatg tcgccgctct gggaaatggc caaggaaggc
    21421 atcgacctga attcggtgca gtgggctcaa cattgacgcg ttgcgtcaat attgagccct
    21481 gcggggga
//
LOCUS       DASWPO010000065        10225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1710306, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000065 DASWPO010000000
VERSION     DASWPO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710306"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2030)
                     /gene="gcvP"
                     /locus_tag="VGC69_04015"
     CDS             complement(<1..2030)
                     /gene="gcvP"
                     /locus_tag="VGC69_04015"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY0294485.1"
                     /translation="MPRALDPHTDFIPRHIGPNEADQAKMLAAVGSPSLDALMGEVVP
                     PGIRLDQPLNLPAPRDEADVLAELKTIAGRNQVLRNYIGQGYYGTHTPNVILRNILEN
                     PAWYTAYTPYQPEISQGRLEALLNYQTMVADLTGLDIANASLLDEGTAAAEAMTLARR
                     SAKSRSNVFFVSGHCHPQTIEVVRTRAEGLGIEVVVGDESKGLPECYGVLVQYPHSLG
                     GVADYKALAEAVHAKGGVVACATDLLALALLAAPGAWGADIAIGSAQRFGVPFGFGGP
                     HAGFMACKDAHKRNMPGRLVGVSKDAQGNNALRLALQTREQHIRREKATSNICTAQVL
                     LAVMAGMYAVWHGPQGIVRIAERVQRYTAILREALVMLGVRVGNDTFFDTLLLHTGSN
                     TGAIVAAAEAAGINLRRVSSTNLAVSLDETVTAHDLQALVGVFGAGLQKSVGMIDVGA
                     LAAKVEGGIPAALRREGPILSHAIFSSVSSETDMLRYLRKLADKDLALDRSMIPLGSC
                     TMKLNATAEMIPITWPEFANLHPFAPVSQSAGYAELIERLSAALCEITGYDNISLQPN
                     SGAQGEYAGLLAIRGYHQANGQAQRNVCLIPSSAHGTNPASAHLAGMDVVVVASDDKG
                     NVDIADLKARIAQVGDKLAALMITYPSTHGVFEEAITEICELVHAAGGQVYM"
     gene            complement(2108..2479)
                     /gene="gcvH"
                     /locus_tag="VGC69_04020"
     CDS             complement(2108..2479)
                     /gene="gcvH"
                     /locus_tag="VGC69_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY0294486.1"
                     /translation="MSLPTDRKYTESHEWIKAEGDVFLVGITGNAQEQLGDIVFVGDV
                     NVGAKLGAGDAAAVVESVKAASDIYAPVAGEILAFNEALDANPSLINESPYDAWIFKI
                     KADNAADIDGLLDAAGYDAAN"
     gene            complement(2526..3626)
                     /gene="gcvT"
                     /locus_tag="VGC69_04025"
     CDS             complement(2526..3626)
                     /gene="gcvT"
                     /locus_tag="VGC69_04025"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY0294487.1"
                     /translation="MSAPLLRTPLAEEHIAAGARMVDFGGWDMPVSYGSQIEEHHAVR
                     RASGMFDVSHMLNVDVTGEGAHAFLSKLIANDVAKLTVPGKALYSCMLNPQGGVIDDL
                     IVYFFAVDTWRVVVNAGTADKDVAWMRRVAASGKFDVVITPRRDLAMVAVQGPEARAK
                     VWAVRPAWKAASETLVPFSAAALDGDVLVARTGYTGEDGFEIVLPATEVASLWRDLLV
                     AGVRPCGLGARDTLRLEAGMNLYGQDMDELIHPDQAGLTWTVSAKDAARRFIGRDAIE
                     QFATPAVFLGLKLLERGVMRSHMKVQTALGEGEVTSGTMSPTLGVSVGFARMPVGIKP
                     GDTVLVDIRGKWLPALVSKLPFVRNGKAVEHA"
     regulatory      complement(3718..3845)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(3866..3964)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3992..4153)
                     /locus_tag="VGC69_04030"
     CDS             complement(3992..4153)
                     /locus_tag="VGC69_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294488.1"
                     /translation="MSTLNRLTAEKIRAEIKKLSAETDRLYMETRWYPMLLAAGLFGA
                     VAAVLKLFL"
     gene            complement(4294..5532)
                     /locus_tag="VGC69_04035"
     CDS             complement(4294..5532)
                     /locus_tag="VGC69_04035"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0294489.1"
                     /translation="MSPSPSDSFDVAVIGAGAAGMMCAAVAGQRGLRVVLLDHAGQLA
                     EKIRISGGGRCNFTNMGAGPGNFLSENPHFCRSALAGYTPQDFLDLVRRHGIAWHEKH
                     RGQLFCDDSSVAIIEMLRGECGQGGVSWRMPCEVREIARDEAGYLLRTSQGRVRAAKL
                     VIATGGMAIPQLGATDFGLKAARQFGLKVVEPRPALVPLTFDAAQWQPFAELSGAALE
                     VELQTGQGKARGRFVEDLLFTHRGLSGPAILQISSYWNPGQAIEIDLAPGRDMAGELL
                     ALKPGSKQQLHTVLAGLWPKRLADRWLASADPAGSPGMAVADLAAARLADLPDKILRE
                     LGARIHRWTLVPTGTAGYKKAEVMRGGVDTRGLDQKSMQAKAVPGLYFIGETVDITGW
                     LGGYNFQWAWASGVACGRAL"
     gene            complement(5570..6394)
                     /locus_tag="VGC69_04040"
     CDS             complement(5570..6394)
                     /locus_tag="VGC69_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0294490.1"
                     /translation="MTTAISRKNPTAAKRPQSDKLFVLSVGKCFQLLECLNAAQRPLT
                     LTELVQQSGLERSAAQRFAHTLRELGYLRQHPQTRAYALSSRMLEFGHTVMMTHSIKE
                     VADPHLLWLNQRCSETVNLMELEGREIVYVSRYPSVHPVSVNLHVGSRLPAFCTAAGR
                     AMLAFMPEPQVLDIFKGPRQAMTQHTVTDIDALQAILAQVRQDGYVINNQEAFIGDIS
                     LAAPIMNAEQHVLGAINIAVPSPRWSVEKSRKELAPLVLDCARRISRELASGRASR"
     gene            complement(6447..7655)
                     /locus_tag="VGC69_04045"
     CDS             complement(6447..7655)
                     /locus_tag="VGC69_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0294491.1"
                     /translation="MRTIYKNATIIDCVNPESMPSGAVVVEDGKISGIFPGDAWTPAE
                     QDAVVDLNGAYLLPGLWDVHIHPDYYSSAPPNVADQTTLFGHRMQAALLDAGIMGMRC
                     CGTHNYLDVAWRDAFNSGQYVGPRLFASGHFLTTTGGHFLGSGHALECDGPYGFVKAI
                     REQIKHGVDHIKLNLSGGIVGPKWDLHTQSFYLDEELRAAFEICKTRGFKIVAHATHP
                     SVVKEAIRLGAHSIEHGYIMDDECIELLLKHDTWLVPTLAISHLTPKQATNKWESDFA
                     FGKGWSHALCCRAEAAAPTHSDWFQRAVKAGVKMALGSDIRPLKEAALLEMGLWTRNG
                     ASTWQTLQAATKHGAAICGVGDEMGTIEVGKIADMIVVKADPLKDINNLRQLELVIKH
                     GKIISDKRKG"
     gene            7845..8819
                     /locus_tag="VGC69_04050"
     CDS             7845..8819
                     /locus_tag="VGC69_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294492.1"
                     /translation="MIRKFAALIGAAALSAAMASTAHADDYPAHPVRIIVAYPPGGST
                     DVAARLLAERLTTTLKQSFIVENRPGAGGVIGANAVAKSPADGYMLLFAASPELSIAG
                     LTTPDLPYDANKDFAPITLVGQVPFILVANNDFPPNNVKELIAYAKANPGKVNFSSFG
                     NNTSNHLGGELFSQQAGISMTHVPYRGSAPSLTDLMGGQIQITFDTITAVYPLVQSHK
                     IKALAVATPERSPLAPDLPTVSESGLPGFVAGTWFGLLAPAGTPQSVVDKLAAATSTI
                     LKSDTIRKEFASRGIQPSPTTPAEMHAFVANEISKWTEAMKKIGTKTN"
     gene            8822..10072
                     /locus_tag="VGC69_04055"
     CDS             8822..10072
                     /locus_tag="VGC69_04055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0294493.1"
                     /translation="MQQTHPDRVAVVGAGIVGLCTAYALRQAGHAVTLFDPNPPGSQC
                     SSGNAGAISSRSVVPLATPGIIKTAFSMLLDAASPLHVPAYYWLQAAPWLARFVAASK
                     PERVRAIANALSDLLRGSVETHTQLAAEVGASDLIRRAGQLHLYPDAAYYAKDQASWA
                     LKQEHGLRIDLLTRPDIEALEPDVGPGYQIGAFLPTEGWVAEPLRYAQAIAARLADMQ
                     AVFVQAAVQSLQADAAGWTLSDGTRRWQAERVVICAGVWSRPLLAAQGYKVPLESQRG
                     YHLHLARPNLDLKHQVVLTDRKVFITPMENGLRMAGTVEFGSLTHPPSMQRALLLGEH
                     ARAGLPTLDTRGAATWMGHRPCLPDSMPVLGPVPGRNGLWCAFGHGHLGLTGSANTGK
                     LIAGAMAGQPDAQRILRPFSIERF"
BASE COUNT         1708 a         3438 c         3330 g         1749 t
ORIGIN      
        1 tccatgtaga cctggccgcc ggcggcatgc accagttcgc agatctcggt gatggcctct
       61 tcgaacacgc cgtgcgtgga cggataggtg atcatcagcg cggccagctt gtcgcccact
      121 tgcgcgatcc tggccttgag gtcggcgatg tcgacgttgc ccttgtcgtc cgaggccacc
      181 acgaccacgt ccatgccggc caggtgggcc gaggcgggat tggtgccgtg cgccgacgag
      241 gggatcaggc agacgttgcg ctgggcctgg ccgttggcct ggtgatagcc gcggatggcc
      301 aggaggcccg cgtactcgcc ctgcgcgccc gagttgggct gcaggctgat gttgtcgtag
      361 ccggtgattt cgcacagcgc ggccgacagg cgttcgatca gctcggcgta gccggcgctc
      421 tgcgacacgg gcgcgaaggg gtgcaggttg gcgaactcgg gccaggtgat ggggatcatc
      481 tcggccgtgg cgttgagctt catggtgcac gagcccagcg ggatcatgct gcggtccagc
      541 gccaggtcct tgtcggccag cttgcgcagg tagcgcagca tgtcggtctc ggacgacacg
      601 ctcgagaaga tggcatgcga gagaatcggg ccttcgcggc gcagcgcggc cgggatgccg
      661 ccctcgacct tggccgccag cgcgccgaca tcgatcatgc ccacgctctt ctgcaggccg
      721 gcgccgaaca cgcccaccag cgcttgcagg tcgtgcgccg tcacggtttc gtcgagcgac
      781 acggcaagat tcgtgctgct gacgcggcgc aggttgatgc cggccgcctc ggccgcggcg
      841 acgatggcgc cggtgttcga gccggtatgc agcagcaggg tgtcgaagaa ggtgtcgttg
      901 cccaccctca cgcccagcat gaccagggct tcgcgcagga tggcggtata gcgctgcacg
      961 cgctcggcga tgcgcacgat gccctgggga ccatgccaca cggcgtacat gccggccatc
     1021 acggccagca gcacttgcgc cgtgcagata ttggaagtgg ctttctcgcg gcggatgtgc
     1081 tgttcgcgcg tctgcagcgc caggcgcagc gcgttgttgc cctgcgcgtc tttcgacacg
     1141 ccgaccaggc ggccgggcat attgcgcttg tgcgcatcct tgcaggccat gaagccggcg
     1201 tgcgggccgc cgaaaccgaa aggcacgccg aagcgctggg ccgagccgat ggcgatgtcg
     1261 gcgccccagg cgccgggcgc ggccagcagg gccagggcca gcaggtcggt ggcgcaggcc
     1321 accacgccgc ccttcgcatg cacggcctcg gccagcgcct tgtagtcggc cacgccgccc
     1381 aggctgtgcg gatactgcac cagcacgccg tagcactcgg gcaggccctt ggactcatcg
     1441 cccaccacca cctcgatgcc caggccttcg gcgcgcgtgc gcaccacttc gatggtctgc
     1501 ggatggcaat gcccggacac gaagaacaca ttgctcctgg acttggcgct gcgccgcgcc
     1561 agcgtcatgg cttcggcggc ggccgtgcct tcgtccagca gcgaggcgtt ggcgatgtcc
     1621 aggccggtga ggtcggccac catggtctgg tagttgagca gcgcttccag gcggccctgc
     1681 gagatctcgg gctggtaagg cgtataggcc gtgtaccagg cggggttctc caggatgttg
     1741 cgcaggatca cattgggcgt gtgcgtgccg tagtagccct ggccgatgta gttgcgcaac
     1801 acctggttgc ggccggcgat ggtcttgagc tcggccagca cgtcggcttc gtcgcgcggc
     1861 gcgggcaggt tcagcggctg atccaggcgg atgcccggag gcaccacttc gcccatcagg
     1921 gcatcgaggc tggggctgcc caccgcggcc agcattttgg cctggtcggc ttcgttgggg
     1981 ccgatatggc gggggatgaa atcggtatgg ggatccaggg cgcgcggcat ggggctatct
     2041 cggaaaacag gggcacgggt tgcgccggac acggccggcg caaccaccat ctagaacggc
     2101 aaggcggtca attggccgcg tcgtagccgg cggcatccag caggccgtcg atgtcggcgg
     2161 cattgtcggc cttgatcttg aagatccagg catcgtaggg cgactcgttg atgagcgagg
     2221 ggttggcgtc gagcgcttcg ttgaaggcaa ggatctcgcc ggccaccggc gcgtagatgt
     2281 cggaagcggc cttcaccgat tccaccacgg cggcggcatc gcccgcgccg agcttggcgc
     2341 cgacattgac gtcgcccacg aagacgatgt cacccagctg ctcctgggcg ttgccggtga
     2401 tgccgacgag gaacacatcg ccttcggcct tgatccattc gtgggactcg gtgtacttgc
     2461 gatcggtggg cagactcatg gtgagctcct gaggggatac gggtttggga ttcgggaatg
     2521 aagggtcagg cgtgctcgac ggccttgccg ttgcgcacga agggcagctt gctcaccagg
     2581 gcgggcagcc acttgccgcg gatgtccacc agcacggtgt cgccgggctt gatgccgacg
     2641 ggcatgcgag caaagcccac cgacacgccc agcgtgggcg acatggtgcc gctggtgacc
     2701 tcgccttcgc ccagtgcggt ctggaccttc atgtgcgagc gcatcacgcc gcgctcgagc
     2761 agcttcaggc ccaggaagac cgcgggggtg gcgaactgct cgatggcatc gcggccgatg
     2821 aagcggcgcg cggcgtcctt cgccgagacc gtccaggtca ggccggcctg gtcgggatgg
     2881 atgagctcgt ccatgtcctg gccgtagagg ttcatgccgg cttccaggcg cagcgtgtcg
     2941 cgcgcgccca ggccgcaagg gcgcacgccc gcgacgagca ggtcgcgcca caggctcgcg
     3001 acttcggtgg cgggcagcac gatctcgaag ccgtcttcgc cggtgtagcc ggtgcgcgcc
     3061 accagcacgt cgccgtccag cgcggcggcc gagaacggca ccagggtctc ggaagccgcc
     3121 ttccaggccg gacggacagc ccacaccttg gcgcgggcct cgggaccctg caccgccacc
     3181 atggccaggt cgcggcgcgg cgtgatgacg acgtcgaact tgcccgaggc ggcgacgcgg
     3241 cgcatccagg ccacgtcctt gtcggccgtg ccggcattga ccaccacgcg ccaggtatcg
     3301 acggcgaaga agtagacgat caggtcgtcg atcacgccgc cctgcgggtt gagcatgcag
     3361 ctgtacagcg ccttgcccgg cacggtgagc ttggccacgt cgttggcgat gagcttgctg
     3421 aggaatgcgt gggcgccctc gcccgtcacg tcgacgttga gcatgtgcga cacgtcgaac
     3481 atgccgctgg cccggcgcac ggcgtggtgc tcttcgatct gcgagccgta ggagacgggc
     3541 atgtcccagc cgccgaaatc gaccatgcgg gcgccggcgg cgatgtgttc ttctgccagg
     3601 ggggtacgta ggaggggtgc ggacatgcgg gagggctccg tcgctgcggt aaggtttgcg
     3661 caataccggt gacggaagcg gaaagcgccc cggacaactc ttgtcaccgg ccggtgtttc
     3721 cgcccctctg tccttttgcc tgagagttgc cccgccgcgc ggccatggcc tgcggcaggt
     3781 ttgcaccttc ggcgcctggg ctgccttgaa tgaaatgggc gccaggtctc tccagagtac
     3841 tgtttcggcg ccgatgaagc cgggccggac aggtgcggta tgggatacct gagcgattct
     3901 gggcgaattg cgccttcggc ggcgggccca agcctaggcg cccgctctct cccgcgacct
     3961 gcgtgacagc ggggtaatta tacctacacc gtcaaagaaa tagcttcaat acggcggcaa
     4021 ctgcgccgaa caaaccggcg gccagcaaca tcggatacca ccgtgtttcc atgtatagcc
     4081 tgtccgtctc ggcggacagc ttcttgattt ccgcgcgtat tttttcggct gtcaggcgat
     4141 tcagggtact catgcgaggg ctccgtcgta tcgtccgcat ggaagggcgt catgcccggt
     4201 ttcgaccacg aacgtaatat acgctggaca aactgcgccg gccattggat ggatttgtca
     4261 cacgaggcaa atccagtacg ctgcccgctc accttacaac gcgcgcccgc aggccacgcc
     4321 cgaagcccag gcccattgga agttgtagcc gcccagccag ccggtgatgt cgacggtttc
     4381 gccgatgaag tacaggccgg gcacggcctt ggcctgcatg gacttctggt ccaggccgcg
     4441 cgtgtccacg ccgccgcgca tgacctcggc cttcttgtag ccggcggtgc cggtggggac
     4501 aagcgtccag cggtggatgc gggcgcccag ctcgcgcagg atcttgtcgg gcaggtcggc
     4561 caggcgagcg gcggccaggt ccgcaacggc catccccggc gagccggccg gatcggccga
     4621 agccagccag cgatcggcca atcgtttggg ccacaggccg gccagcaccg tatgcaattg
     4681 ctgcttgctg cccggcttca atgccagcag ctccccggcc atgtcgcgtc ccggcgccag
     4741 gtcgatctcg atggcctggc ccggattcca atagctcgaa atctgcagga tggccggccc
     4801 cgacaggccg cggtgcgtga acagcaggtc ttccacgaag cggccgcggg ccttgccctg
     4861 gccggtttgc agctcgactt ccagcgccgc gcccgacaac tcggcaaacg gctgccattg
     4921 cgcggcgtcg aaggtcagcg gcaccagcgc cgggcgcggc tcgaccacct tcaggccgaa
     4981 ttggcgcgcc gccttcaagc cgaaatccgt ggcccccagc tgcggaatgg ccatgccgcc
     5041 cgtggcgatc accagcttgg ccgcccgcac cctaccctgg ctggtgcgca gcagatagcc
     5101 ggcctcgtcg cgcgcgatct cgcgcacttc gcaaggcatg cgccagctca ccccgccctg
     5161 cccgcactcg ccgcgcagca tctcgatgat ggctacgctg gagtcgtcgc agaacagctg
     5221 gccgcgatgc ttttcgtgcc aggcaatgcc atggcggcgc accaggtcga ggaagtcctg
     5281 cggcgtgtag ccggccaggg ccgaacggca gaaatgcggg ttctccgaca ggaaattgcc
     5341 cggccccgcc cccatattgg tgaagttgca gcgcccgccg cccgagatgc ggattttctc
     5401 ggcaagctgg ccggcgtggt cgagcagcac cacgcgcagg ccgcgctggc ccgccaccgc
     5461 ggcgcacatc atgccggccg cgccggcgcc gatcacggca acgtcgaagg agtcgctggg
     5521 gctggggctc atggggcgct atttgaacag atcgcgccgg acggccggtt tagcggctcg
     5581 cgcgccccga cgcgagctcg cgcgaaatcc tgcgcgcgca gtcaagcacc aggggggcga
     5641 gctccttgcg gcttttttcc acactccagc gcggcgatgg cacggcgatg ttgatggcgc
     5701 ccaacacgtg ctgctcggcg ttcatgatgg gggcagccag ggaaatgtcg ccgatgaagg
     5761 cttcctggtt gttgatgacg tagccgtctt gacgcacttg ggccaggatg gcctgcagcg
     5821 catcgatatc cgtcacggtg tgctgcgtca tggcttggcg aggccctttg aatatgtcca
     5881 gcacttgcgg ttcgggcatg aaagccagca tggcccggcc cgctgccgtg caaaaggccg
     5941 gcaaacgcga tcccacgtgc aggttgacac tcaccggatg cacgctgggg tagcgcgaca
     6001 catagacgat ctcccgccct tcgagctcca tcaggttgac cgtttcgcta cagcgctggt
     6061 tgagccacag cagatgcggg tcggccactt ccttgatcga atgcgtcatc atgacggtat
     6121 ggccgaattc cagcatccgg ctcgaaagcg cataagcccg ggtctgcgga tgctgccgca
     6181 aatagcccag ttcgcgcagc gtgtgcgcaa agcgctgggc agcgctgcgc tcgaggccgg
     6241 actgctgcac aagctcggtc agcgtcaggg gccgctgcgc ggcattcaga cactccagca
     6301 actggaagca ctttcccacc gagagcacaa acaacttgtc gctctgcggc cttttggccg
     6361 cggtggggtt tttcctggaa atagccgtcg tcatatccct aaaaggcccg aacaaccatg
     6421 gcagccggta tcagcggctg aaatcctcag cctttgcgct tatcgctaat gatcttgcca
     6481 tgcttgatca cgagttcgag ttggcgcagg ttgttgatgt ctttcaaggg atcggccttc
     6541 accacgatca tgtcggcgat cttgccgacc tcgatggtcc ccatctcatc gccgaccccg
     6601 cagatggcgg cgccatgctt ggtcgcggcc tgcaatgtct gccacgtcga ggcgccattt
     6661 ctggtccata ggcccatttc aagcagcgcg gcctctttca atgggcggat atcggagccc
     6721 agcgccattt tcacgcccgc cttgacggct ctctggaacc aatcggaatg cgtgggcgcc
     6781 gcggcttcgg cacggcagca caaggcgtgg gaccaccctt tgccaaaggc gaaatcggat
     6841 tcccacttgt tcgtggcctg cttcggcgtc aaatgactga tggccagcgt gggcaccagc
     6901 caggtgtcgt gcttgagcaa cagttcgatg cattcatcgt ccatgatgta gccgtgctcg
     6961 atgctgtgcg cgcctagccg tattgcttcc ttgacgacgc tcggatgcgt ggcgtgcgcg
     7021 acgatcttga agccccgggt cttgcagatc tcgaaagccg cgcgcagttc ctcgtcgaga
     7081 tagaacgatt gggtatgcag atcccatttg ggaccgacga tgccgcccga aagattcaac
     7141 ttgatgtggt cgacgccgtg cttgatctgc tcgcgtatcg ccttgacgaa accatagggc
     7201 ccatcgcact cgagcgcgtg gcccgacccg aggaagtggc ctcccgtggt ggtcaggaag
     7261 tgcccgctcg caaacaagcg cggcccgaca tactggcccg agttaaatgc atcgcgccat
     7321 gccacgtcca ggtaattgtg cgtaccgcag caacgcatcc ccatgatgcc ggcgtcgagc
     7381 agcgcggctt gcattctgtg gccgaacagg gtcgtttggt cggcgacgtt cgggggcgcg
     7441 ctggaatagt agtcggggtg tatgtgcaca tcccacaggc caggcagcag gtaggcgccg
     7501 ttcaggtcga cgacggcgtc ctgctccgcg ggagtccatg catcgccggg aaagatgccg
     7561 gagatcttcc cgtcctcgac cacgacggcg ccggacggca tggactcggg attgacgcaa
     7621 tcaatgatcg tggcgttttt gtatatggtt cgcatggcac ccctggccgt ttctatgctc
     7681 tttgcattcc tcctctgaat cctatttcag atgatggagg aaatccacaa gtattggtat
     7741 ccaatatcaa gtattgaata acgacatcat atggaatagc gttgacttcg gcaatgaccg
     7801 gcacgaccgc attgccgcac aacaaccgat acaaggggta aaaaatgatc cgcaaattcg
     7861 ccgcactgat cggcgcggcc gcgctgtcgg ccgccatggc ctccaccgcc cacgccgatg
     7921 actatccagc ccatccggtt cgcatcatcg tggcctaccc gcctggcggc agcaccgatg
     7981 tggccgccag gctgctggcc gaacgcctga ccaccaccct gaagcaaagc ttcatcgtgg
     8041 aaaaccgccc cggcgccggc ggcgtgatcg gggcaaatgc cgtcgcgaaa tcgccggccg
     8101 atggctatat gctgctgttc gccgcctcgc ccgaactctc gatcgccggc ctcaccacac
     8161 ccgacctgcc ctacgacgcc aacaaggact tcgccccgat cacgctggtc ggccaggtgc
     8221 cgttcatcct ggtggccaac aacgactttc cgcccaataa cgtcaaggaa ctgatcgcct
     8281 acgccaaggc caatccgggc aaggtgaact tttcctcctt cggcaacaac acctccaacc
     8341 acctgggcgg cgaactgttc agccagcagg caggcatctc gatgacgcac gtgccgtacc
     8401 gcggcagcgc gccctcgctc accgacctga tgggcggcca gatccagatc accttcgaca
     8461 cgatcaccgc cgtctatccg ctggtccaga gccacaagat caaggcgctg gccgtggcca
     8521 cgccagaacg ctcgccgctg gcgcccgacc tgcccaccgt ctccgaaagc ggcctgcccg
     8581 gcttcgtggc aggcacctgg ttcggcctgc tggccccggc cggcaccccc cagtccgtcg
     8641 tcgacaagct ggccgccgcc accagcacca tcctgaagtc cgatactatc cgcaaggaat
     8701 tcgccagccg cggcatccag ccctcgccca ccacgcccgc cgaaatgcat gcgttcgtcg
     8761 ccaacgagat cagcaagtgg accgaggcaa tgaagaaaat cggaaccaag accaactgaa
     8821 catgcagcaa actcatccag accgcgtcgc cgtcgtcggc gcgggcatcg tcggcctgtg
     8881 caccgcctat gccctgcggc aggcgggcca tgccgtcacg ctgttcgacc ccaacccgcc
     8941 cggctcgcaa tgctcgtcgg gcaatgccgg ggcgatctcg tcgcgctcgg tggtgccgct
     9001 ggccacgccc ggcatcatca agaccgcctt ctcgatgctg ctcgacgccg ccagcccgct
     9061 acacgtgccg gcctactact ggctgcaggc cgcgccctgg ctggcccgct tcgtcgccgc
     9121 ctccaagccc gagcgggtgc gcgccatcgc caacgcgctg tccgacctgc tgcgcggctc
     9181 ggtcgagacg cacacgcaac tggccgccga agtcggcgcc agcgacctga tccgccgcgc
     9241 cggccaattg cacctttacc cggacgcggc ctattacgcc aaggaccagg catcctgggc
     9301 cctcaagcaa gagcatgggc tgcgcatcga tctgctcacc cgccccgaca tcgaagccct
     9361 ggaacccgat gtgggcccgg gctaccagat cggcgccttt ctgcccaccg aaggctgggt
     9421 ggccgagccg ctgcgctatg cgcaggccat cgcggcgcgc ctggccgaca tgcaggccgt
     9481 gttcgtgcag gccgccgtcc aatccctgca ggccgatgcc gcgggctgga cgctgtcgga
     9541 cggcacccgc cgctggcagg ccgaacgcgt ggtgatctgc gccggcgtgt ggtccaggcc
     9601 cttgctggcc gcccagggct acaaggttcc cctggaaagc cagcgcggct atcacctgca
     9661 cctggcgcgg cccaacctcg atctcaagca ccaggtggtg ctgaccgacc gcaaggtgtt
     9721 catcaccccc atggagaacg gcctgcgcat ggccggcacg gtggagttcg gcagcctgac
     9781 gcatcccccc agcatgcagc gcgccctgct gctgggcgaa cacgcccggg ccggcctgcc
     9841 gacgctggac acccgcggcg ccgccacctg gatgggacac cgcccctgcc tgcccgactc
     9901 catgccggtg ctgggccccg tgcccggccg caacggcctg tggtgcgcct tcggccacgg
     9961 ccacctgggc ctgaccggat cggccaacac gggcaagctg atcgccggcg ccatggccgg
    10021 ccagcccgat gcccagcgca tcctcaggcc gttctcgata gaacgctttt gattcgagcc
    10081 tgagactctc tcagcatcgg agccgagggc gcaaagcgcg caggcgccta tgaattgggg
    10141 cagcccccgc agggggccga gggcgcctgc atgcgcccga gcccaattca tcgaagccgg
    10201 agcgctttgc gccctcggct ccgat
//
LOCUS       DASWPO010000066        13914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_103754, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000066 DASWPO010000000
VERSION     DASWPO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13914
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103754"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2064
                     /locus_tag="VGC69_04060"
     CDS             <1..2064
                     /locus_tag="VGC69_04060"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HEY0294494.1"
                     /translation="DTSHGMELMRPLMPNGTASSRPGVLLPYAGMLIKTKQDAEAANV
                     LRQLQNQNLTRDQRHQFNDLVNLYAVRLADRQRQQGNLAAAYDTLTPMMAKRPNDNLV
                     AGAMARLYTSDGKYDEALDLYKQMQARDPDNANVQISTAFAASQVQDYGYADQALDHA
                     VAVAPDDPDVLAGAARVYLMRGKSGKAATLLESAIAIKEKRLAAQPAQPTQLAAAPSR
                     AETPANPAAVASEAAARAPAPGPVAASEKAISPVPASASSATYTQPRPSTAAPVVVSA
                     TAQDDDPSSLATMKSELIGIREARSPEIRAGVFMQSNNGASGTSKLTALQEPMEARMP
                     VGEGKLVVRVTPVQLKSGSVGSDAYSSSQFGGGPVAAQAQAAGSVGAPRSQRASGVGL
                     ALGYEARDWMADLGTTPLGFEHTNVVGGAELRGAINPQKGSWYTVGASRRAVTDSVTS
                     FAGTRDSRTGDSWGGVTATGVHGQIGVDRPAGGAYAYGSFASLNGKNVASNTRAEVGT
                     GVYWYLQRSANSLLTAGLNLGGMFYNKNESSFTYGNGGYFSPQSFYSLSVPVTWAQRS
                     DRFSYKLQGSVGLQHYRASSEPYFPTSSAMQSAAVSAASAIGLSDNAIHPGQSSTGIG
                     YSLSAAGEYRLTRNLTMGAVAGMNNASSYRQWVGGLYLRYSFYPQTQSMDMPVAPYHS
                     PYSSQ"
     gene            2092..2751
                     /locus_tag="VGC69_04065"
     CDS             2092..2751
                     /locus_tag="VGC69_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0294495.1"
                     /translation="MLASVLAGLSILIVGDSHLGSNGYLIDTLHDALLADGAQVHTIG
                     VCGTNPGDWVQSVQGTCGGAERRGKAPLVKQNPAKTQPIGQLIDADKANLVIVIMGDT
                     IGGYKQDAFPKTWVWQQVTSFTKVVAEHKTACVWVGPAWGSEGGKYGKTYAKAQQISS
                     FLSNNVAPCSYVDSLKFSKPGEWGTIDGQHLTLTAYKYWGKDIEQAIVQIPALQQLKR
                     P"
     gene            2782..3438
                     /locus_tag="VGC69_04070"
     CDS             2782..3438
                     /locus_tag="VGC69_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0294496.1"
                     /translation="MAVSALVGLSLLVIGESHITYNLREPLMQALLAQGAKHVHAVGA
                     CGASPDRWLTKNPVDCGADQVDNKPAVVLKRDAFTTPITDLINADKPDLVVVIIGDTM
                     GSYDKPTFAKTWAWQGTTGLTKAIASTNTACVWVGPGWGTESGSFAKNFNRVKELNAF
                     LAANVEPCSYIDSTTFSKPGAWPTVDGEHYTQAGYAAWSKGIVQSLAVSPTVLKLKKK
                     "
     gene            3435..4112
                     /locus_tag="VGC69_04075"
     CDS             3435..4112
                     /locus_tag="VGC69_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042121 - alginic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase AlgF"
                     /protein_id="HEY0294497.1"
                     /translation="MKKTLTCLALACLGAHAAPMAGAAEIPLYESGPAEDSSFIRFVD
                     AGTQAVEVTAAGSKAKLALDASKPASDFLPVRAGKPIEGTLTRGSAHQDVSATVKPGE
                     FVSVVALEGEGGALSVVTMHESPDDFNATKASVALYNLDARCGAPALKVAGRDIVLLD
                     QVAEGQSKRRQINPVALGVQLVCGAQAVGQPLDLGTLVAGQRYTVFLVPAGQGSRIFY
                     ATDSVAH"
     gene            complement(4159..5016)
                     /locus_tag="VGC69_04080"
     CDS             complement(4159..5016)
                     /locus_tag="VGC69_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0294498.1"
                     /translation="MPIASTSPTFLQALTRVLAATLLCLAPWLARAESVAITFDDGLD
                     PRSEPRAVQWNARILSALAKHHVRTMFFPAGVVVDSPEGLALVRAWGEAGHAIGNHTY
                     SHQGFLDTTSPQAFMQDILREQALVGGMPGWCPRIRLPYLNEGNTPERRNQFFELLAQ
                     HGYGEAPVTLGIDDWNYSERFVNALNQNPNMDIAPFRKAYLDRWQQELRKQAAEWRQR
                     LGRSPVHVMLLHTNGLNATVLSDLLAMMEADGWTFVDPAQAFTDYIYQRGYPKSDGTI
                     VPLPVPACR"
     gene            5127..6536
                     /locus_tag="VGC69_04085"
     CDS             5127..6536
                     /locus_tag="VGC69_04085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY0294499.1"
                     /translation="MVFASLEFLTLFLPAFLAIYTLSPARWRNAVLLFCSWFFYGWWS
                     PVFLLLFIGLTLWGWLGGLVIEAAPGPRSRGWRLAFFIAVNVLILCWYKYANIVVESL
                     DAALAMAHAGPIAWQRVLLPIGLSFIVLQSISYLIDVYRTTVPADRSFIRYGAYQAMF
                     VHLIAGPIIRYAWVKRELASRTVGWDGFAAGARRLMIGMSMKVLVADTLSPMVDAIFS
                     LPSPSMADAWLGCGFYTLQLFFDFAGYSAMAIGLGQILGFRFPENFNHPYLASSIQDF
                     WRRWHLSLSSWIRDYLYIPLGGSRLGEWKTYRNLILTMAIAGLWHGGDSWNFLLWGIA
                     HGLALALARLWRQMGMPSLPLWLSHLMTLLFVMLAWTLFRSVGFEAALTMYRGQFGLN
                     GIGLSDAMAVQLRPVHGLATALGIVCVLLPIWQQRWDSLPDTALRRFWQAAWPLAGFL
                     LSFALIAARGAVPFLYFQF"
     gene            6555..7730
                     /locus_tag="VGC69_04090"
     CDS             6555..7730
                     /locus_tag="VGC69_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HEY0294500.1"
                     /translation="MADLMLLPQLPATFASKAAAVGISVFLLAGLVSNVWLASTSDKP
                     LLTSAATRERVLGGTVSHELADRMADSPLPFESARVQRGLNWLAFGDLGPRVRQGCPG
                     WLFIRDELMVYRDASAHAATRAQTVAAVQRDLASRGIKLLVIIVPDKSRIEAQHLCGL
                     HRPASLDGRVNDWMAQLAAQGVRAVDLAPALRGVQGDAYFHTDTHWTEAGAGAAAQAV
                     AQRVHQLGLALPPGGAYKVEAQPVARRPGDLVHLAGLDWLPPSMQPEPETAQAHTYTP
                     EAKAAATDDDLFGDAGLPIVSLLGTSYSRNSNFAPQLAMALGSGVNNFARDGGMFGGA
                     AQAYFPSQAYTQTPPKLIVWEVDERDLQAPLLPADAVAVPLASPAAPGAAGGARASQ"
     gene            7757..8512
                     /locus_tag="VGC69_04095"
     CDS             7757..8512
                     /locus_tag="VGC69_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY0294501.1"
                     /translation="MATRRRFLTALPLLGSPVLAGAATSRAYAVVIGDSIAEGEPNRH
                     GRLNVNGQFVPDYPSQPGQLSYELAKYSGVFHVNHGIGGQVAAQVRQRWPRDVLAEDL
                     DPGDGRGSRTLPKGRLPALVYLHVGINDIAARVPLQSMQDSFTYFAQTTQGRGLPLVI
                     DNIGSWLGMPPPMVDEARAFNTWLVEELAPRHQNIVVADYLDWSSGNTGDYRVMAPGK
                     FVDGLHPTMEGYQDFARYVHDTVKLPARSAVHY"
     gene            complement(8506..9363)
                     /gene="folD"
                     /locus_tag="VGC69_04100"
     CDS             complement(8506..9363)
                     /gene="folD"
                     /locus_tag="VGC69_04100"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY0294502.1"
                     /translation="MTARLIDGKALSEKVRAEVALRVTALKSKGITPGLAVVLVGEDP
                     ASQVYVRNKVAACEQAGLHSVKEQYPATLSEAELLARIDALNADPAIHGILVQLPLPK
                     HMDAHKVIEAIAAEKDVDGFHVSNAGLLMTGQPLFRPCTPYGVMKMLESEGTALRGAE
                     AVVVGASNIVGKPMAMLLLQAGATVTLCNSKTRDLAAQARRADVLVVATGKPKMVTGD
                     MIKPGAVVIDVGINRGADGKLCGDVDFDSAAAVAGAITPVPGGVGPMTIAMLLVNTVE
                     AAERAARNQ"
     gene            complement(9776..10402)
                     /locus_tag="VGC69_04105"
     CDS             complement(9776..10402)
                     /locus_tag="VGC69_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0294503.1"
                     /translation="MNTPPMSSTVFIVDDDEAVRDSLRWLLEANGYRVRPYASAETFL
                     DEYDPNLIGVLIADVRMPGMSGLELQEQLIARKAPLPIVFITGHGDVPMAVSTMKKGA
                     VDFLEKPFNEADLRDIVARMLEQAVQRVNQFQAQRDHEAMLARLTAREQQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLEVTTVADLMKVALAKPEAHA"
     gene            complement(10399..12150)
                     /locus_tag="VGC69_04110"
     CDS             complement(10399..12150)
                     /locus_tag="VGC69_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0294504.1"
                     /translation="MSSPKSHIPTPFHDDARMRRRGLYWLTPALVLVLYLCVMGVFFW
                     LQRIRDDSVMFVTIDQELRQQRLLWVVIALSCVIVVSLLMLWRYTRFRSEAEAALIAE
                     TGFRRAMENSMSTGMRVLDMEGRIAYVNPAFCRMIGWNEADLIGRSPPFPYWVPGRHD
                     QHQHTLDVLMSGKTPSSGLEIEAQRRDGSRFAARMYVSPLLDPNGNQIGWMTSMTDIT
                     EPKRIREALTAAHERFMTVLEGLDDAISVTADTHDGLELLFANRTYRRVFGAQTGGHD
                     ELLAGRRGRFTDESVEVFAPSVQRWFEVHHRMLAWTDGRRVRMQVARDITERRQHEEA
                     SRVQQEKIQITSRLTTMGEMASSLAHELNQPLTAISNYSMGAVALVKAGHTSPNMLLP
                     ALEKAAAQAERAGKIISRIREFVKRSEPRRQAVPIRRIVDNAIGFAEIDARKRRIRID
                     SFVPSTAPDVLADPILIEQVLLNLIKNGMEAMEKSESDELKVAVILHEQHLEVAVVDR
                     GHGIAEPERLFEPFFSTKTQGLGMGLNICRTIIESHHGRLWADPNPSGGTIFRFTLPT
                     VDPKTQMQNDQSEELPA"
     gene            12373..>13914
                     /gene="aceE"
                     /locus_tag="VGC69_04115"
     CDS             12373..>13914
                     /gene="aceE"
                     /locus_tag="VGC69_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811941.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY0294505.1"
                     /translation="MSSSVQAGAPAAANDDDNLETQEWLEALAAVLDREGPDRAHFII
                     ERLIDQARRSGVHIPYSPNTAYVNTIPTALEPRHPGNLELEARIRAYIRWNAMAMVVK
                     ANKHNPPDGGDLGGHIASFASLATMIDCGQNHFWHTESAEHGGDLVYFQGHSSPGMYA
                     RAFLEGRLSEEQLDNFRQEVEGKGLSSYPHPKLMPEFWQFPTVSMGLGPLMAIYQARF
                     LKYIHARGIADTSKRKVWVFCGDGEMDEPESLGAIALAAREKLDNLVFVINCNLQRLD
                     GPVRGNGKIIQELEGDFRGSGWNVIKLIWGGYWDPLLAADKEGILRRVMEETVDGEYQ
                     AYKANDGKFVREHFFGKNPKLLEMVARMSDEDVWRLNRGGHDPHKVYAAFDAAFKHTG
                     QPTVILAKTIKGYGMGPVGQAKNPTHQQKKLELESIREFRDRFGIPIADDDLAKVPYF
                     KPAADSPEMQYMQAQRKTLGGYLPKRRTKADEHLIVPGLDAFKAVLDPTAEGREISTT
                     QAFVRT"
BASE COUNT         2256 a         4638 c         4625 g         2395 t
ORIGIN      
        1 gatacctcgc acggcatgga gctgatgcgt ccgctgatgc cgaacggcac ggcatccagc
       61 cgccctggcg tgctgctgcc ctatgccggc atgctgatca agaccaagca ggacgccgag
      121 gccgcgaacg tgctgcggca attgcagaac cagaacctga cgcgggacca gcgccaccag
      181 ttcaatgatt tggtcaacct gtacgcggtg cggctggccg accggcagcg ccagcagggc
      241 aacctggcgg ccgcctatga caccttgacg cccatgatgg ccaagcggcc caacgacaat
      301 ctggtcgcgg gcgccatggc gcgcctgtac acttccgacg gcaagtacga cgaggcgctg
      361 gatctgtata agcagatgca ggcgcgcgac ccggacaacg ccaacgtgca gatcagcacc
      421 gcgtttgcgg cgtcgcaggt ccaggattac ggctacgcgg accaggctct ggatcatgct
      481 gtcgccgtgg cgccggacga tcccgacgtc ctggccggcg cggcgcgcgt ctacctcatg
      541 cgcggcaaga gcggcaaggc ggccacgctg ctggaatccg ccatcgcgat caaggaaaag
      601 cgtctggcgg cgcagcctgc gcagccgacc caactggccg cggcgcccag ccgcgccgag
      661 acgccggcca atccggcggc tgtcgcatcc gaggccgccg ccagggcgcc tgcgcctggt
      721 cccgtcgctg ccagcgagaa ggccatatcg ccggtgcccg cctcggcatc gtcggccacc
      781 tatacccagc cgcgcccgtc taccgcggcg ccggtggtcg tgtccgcgac ggcgcaggac
      841 gacgacccgt cttcgctggc aaccatgaag agcgagttga tcgggatccg cgaggcgcgt
      901 tcgccggaaa tacgggcggg cgttttcatg cagtcgaaca atggcgcgtc cggcaccagc
      961 aagctgaccg ccttgcaaga gccgatggaa gccagaatgc cggtgggcga gggcaagctg
     1021 gtcgtgcgcg tgacgccggt gcagctcaag tccggttcgg tgggcagcga tgcctacagc
     1081 agcagccagt tcggcggcgg accggtcgcc gcgcaggcgc aggcggccgg cagtgtcggc
     1141 gcgcccaggt cgcagcgcgc cagcggcgtc ggcctggcct tgggctacga ggccagggac
     1201 tggatggccg acctcggcac cacgccgctg ggcttcgagc acaccaatgt cgtcggcggc
     1261 gccgagctcc gcggcgccat caatccgcag aagggcagct ggtacaccgt aggcgcgtcg
     1321 cgccgggccg tgaccgacag cgtgacgtct ttcgccggca cgcgcgattc gcgcaccggc
     1381 gacagctggg gcggggtcac cgccacgggc gtgcatggcc agattggcgt cgaccgtccg
     1441 gccggcggcg cctacgccta cggctccttt gcatccctca acggcaagaa cgtcgcgtcc
     1501 aatacccgcg ccgaggtggg gaccggcgtg tactggtacc tgcagcgcag cgcaaacagc
     1561 ctgctgaccg cggggctgaa cctgggcggc atgttctaca acaagaacga aagctccttc
     1621 acctacggca acggcggcta tttcagtccc cagtcgttct attcgctcag cgtgccggtt
     1681 acctgggcgc agcgttccga ccggttcagc tacaagctgc agggctccgt gggcctgcag
     1741 cattaccggg cgagcagcga accgtatttc ccgaccagca gcgccatgca aagcgcggcc
     1801 gtctccgcgg cctccgccat cgggttgagc gacaacgcga tccatccggg ccagtcctcg
     1861 accggcatcg gctacagcct gtcggccgcg ggcgagtacc ggctcacgcg caacctgacc
     1921 atgggcgccg tggccgggat gaacaacgcc tccagctacc ggcagtgggt gggcggcttg
     1981 tacctgcgct acagcttcta tccccagacg caatcgatgg acatgccggt tgcgccctac
     2041 cactcgccat acagcagcca ataagcacgg tttcatccag gagttttatt catgcttgca
     2101 tctgtacttg cgggtctcag cattctcatc gtcggcgaca gccacctggg atcgaacggc
     2161 tatctcatcg acaccctcca cgatgcgctg ctcgccgatg gcgcgcaggt gcacaccatc
     2221 ggcgtgtgcg gcaccaaccc tggcgactgg gtgcagtcgg tgcaaggcac ctgcggcggg
     2281 gccgaacggc gcggcaaggc gcccctggtc aagcagaacc cggccaagac ccagcccatc
     2341 ggccagctga tcgacgccga caaggccaac ctggtcatcg tgatcatggg tgacaccatc
     2401 ggcggctaca agcaggatgc cttccccaag acttgggtct ggcagcaggt gacgtcgttc
     2461 accaaggtcg tggccgagca caagacggct tgcgtctggg tcggcccggc ctggggcagc
     2521 gaaggcggca agtacggcaa gacctacgcg aaagcccagc agatctcgtc ctttctctcg
     2581 aacaatgtcg cgccgtgctc ctacgtcgat tcgctcaagt tttccaagcc gggcgagtgg
     2641 ggcacgatcg acggccagca tttgacgctg acggcctaca agtattgggg caaggatatc
     2701 gaacaggcga tcgtacagat tccggccctc cagcaactga agcggccctg atccgtcggg
     2761 ccgcgtattc aaggaggttt catggctgtt tccgctttag tgggtctttc actgctggtc
     2821 atcggcgaaa gccacattac ctacaacctg cgcgagccgc tgatgcaggc gctgctcgcg
     2881 caaggggcca agcacgtgca cgcggtcggc gcctgcgggg cgagcccgga tcgctggctc
     2941 acgaagaatc cggtcgattg cggcgcggac caggtcgaca acaagccggc cgtggtgctc
     3001 aagcgcgacg ccttcaccac gcccatcacc gacctgatca acgccgacaa gcccgacctg
     3061 gtggtggtca tcatcggcga cacgatgggt tcgtacgaca agccgacctt cgccaagacc
     3121 tgggcctggc agggaaccac cgggctgacc aaggccatcg ccagcaccaa cacggcctgc
     3181 gtctgggtgg ggccgggctg gggcaccgag agcggttctt tcgccaagaa cttcaaccgc
     3241 gtgaaagaac tcaatgcctt tctggcggcc aacgtcgagc cctgttccta catcgattcg
     3301 acgacctttt ccaagcccgg cgcctggccg accgtggacg gcgagcacta cacgcaagcc
     3361 ggctatgcgg cctggagcaa gggcatcgtc caatccctgg cagtctcgcc caccgttctc
     3421 aaattgaaga aaaaatgaag aagacgctga cctgcctggc cctggcctgc ctgggcgcgc
     3481 atgccgcgcc gatggccggg gccgccgaga ttccgctgta cgagagcggc cccgcggaag
     3541 actcgtcgtt catccgcttc gtcgacgccg gcacgcaggc cgtggaagtg accgcggcgg
     3601 gctcgaaggc gaagcttgcg ctcgacgctt cgaagccggc ttccgacttc ctgcccgtgc
     3661 gcgccggcaa gcccattgaa ggcacgctga cgcgaggttc ggcacaccag gacgtgtccg
     3721 ccacggtgaa gcccggtgaa ttcgtcagcg tggtcgcgct ggaaggcgag ggcggggcct
     3781 tgagcgtcgt gacgatgcac gagtcgccgg acgatttcaa cgcgaccaag gcctcggtgg
     3841 cgctctacaa cctcgatgcc cgatgcggcg cgccggcctt gaaggtggcg gggcgcgata
     3901 tcgtgttgct ggaccaggtg gccgaaggcc agagcaagcg ccgccagatc aaccccgtgg
     3961 ccctgggcgt gcagctggtg tgcggcgcac aagccgtcgg ccagccgctg gacctgggta
     4021 cgctcgtggc cggccagcgc tatacggtgt ttcttgtgcc ggcggggcag ggttcgcgga
     4081 ttttttatgc gaccgactcg gtggcccatt aagcgggttg tatcggctcg ccgatcccag
     4141 gcgccgcgcc gcaaggcgtc agcggcatgc gggcacgggc agcggcacga tggtgccgtc
     4201 gctcttcggg tagccgcgct ggtagatgta gtcggtgaag gcctgggccg ggtcgacgaa
     4261 ggtccagccg tcggcctcca tcatcgccag caggtcggac agcaccgtgg cgttcaggcc
     4321 attggtgtgc agcagcatga cgtgtaccgg gctgcggccc aggcgttggc gccattcggc
     4381 ggcctgcttg cgcagctcct gctgccagcg gtcgaggtag gccttgcgga acggcgcgat
     4441 gtccatgttg gggttctggt tcagcgcgtt gacgaaacgt tcgctgtagt tccagtcgtc
     4501 gatgccgagg gtgacgggcg cttcgccata gccatgctgg gccagcaact cgaaaaactg
     4561 gttgcggcgc tcgggcgtgt tgccttcgtt gaggtaaggc aggcgaatgc gcgggcacca
     4621 gccgggcatg ccgcccacca gggcctgctc gcgcaggatg tcctgcatga aggcctgggg
     4681 cgaggtggtg tcgaggaagc cctggtggct gtaggtgtgg ttgccgatgg cgtggcccgc
     4741 ttcgccccag gcgcgcacca gcgccagccc ctcggggctg tcgaccacga cgcccgccgg
     4801 aaagaacatg gtgcgcacat ggtgcttggc cagggcgctg agtatgcggg cgttccattg
     4861 caccgcgcgc ggttcggagc gcgggtcgag cccgtcgtcg aaggtgatgg cgaccgactc
     4921 cgccctggcc agccagggcg ccaggcaaag caatgttgcc gccagcacgc gggtcagcgc
     4981 ttgcagaaaa gtcggggagg tcgatgcaat cggcatgccc cgtattttag gcgtgtcgcc
     5041 ttgcgcactg gcgagttgat acaaacattt ttctttattc gtttgctttc ctccatttca
     5101 tcctctgcta ctaaggaacc gcgagcatgg tctttgcgtc cctggaattc ctgacgcttt
     5161 tcctgccggc cttcctggca atctacacac tgtccccggc acgctggcgc aatgccgtgc
     5221 tgctcttttg cagctggttc ttctatggct ggtggagccc ggtttttttg ctgctcttca
     5281 tcgggctgac cttgtggggc tggctgggcg gcctggtcat cgaggccgcg cccgggccac
     5341 gttcgcgcgg ctggcggctg gcttttttca tcgccgtcaa cgtgctgatc ctgtgctggt
     5401 acaagtacgc caacatcgtg gtcgaaagcc tcgatgccgc gctggccatg gcgcacgccg
     5461 gacccatcgc ctggcagcgc gtgctgctgc ccattgggct gtccttcatc gtgctgcagt
     5521 ccatctcgta cctgatcgac gtgtaccgca ccacggtgcc ggccgaccgc agcttcatcc
     5581 gctacggcgc ctaccaggcc atgttcgtcc acctgatcgc cggtcccatc atccgctatg
     5641 cctgggtcaa gcgcgagctg gcctcgcgca cggtgggctg ggacggcttc gccgccggcg
     5701 cgcggcgcct gatgataggc atgagcatga aggtgctggt ggccgacacg ctctcgccca
     5761 tggtcgacgc catcttctcg ctgccatcgc ccagcatggc cgacgcctgg ctgggctgcg
     5821 gcttctacac gctgcagctc ttcttcgatt tcgcgggcta tagcgccatg gccatcggcc
     5881 tggggcagat actgggcttc cgctttcccg agaacttcaa ccatccctat ctggcctcca
     5941 gcatccagga cttctggcgg cgctggcacc tgtcgctgtc gagctggata cgggattatt
     6001 tgtacatccc gctgggcggc agccgcctgg gcgaatggaa gacctaccgc aatctgatac
     6061 tgaccatggc catcgccggc ctgtggcacg gcggcgacag ctggaacttc ctgctgtggg
     6121 gcatcgccca tggcctggcg ctggcgctgg cgcgcctctg gcgccagatg ggcatgccgt
     6181 ccctgcctct atggctgtcg cacctgatga ccctcctttt cgtcatgctg gcctggacgc
     6241 tgttccgctc ggtgggcttc gaggcggccc tgaccatgta ccgcggccag ttcggcttga
     6301 acggcatcgg cttgagtgac gccatggccg tgcaactgcg gcccgtgcac ggtctggcca
     6361 cggcgctggg catcgtctgc gtgctgctgc cgatctggca gcagcgctgg gacagcctgc
     6421 ccgacaccgc gctgcgccgc ttctggcagg ccgcctggcc gctggcgggc tttctgctgt
     6481 cgttcgcgct gatcgccgcg cgcggcgccg tgcctttcct ctatttccag ttctgatatc
     6541 cgggttccga cgccatggcc gatctcatgc tgcttcccca gttgcccgcc acgttcgcca
     6601 gcaaggccgc tgcggtgggc atttccgtct tcctgctggc gggcctggtt tccaacgtct
     6661 ggcttgcgtc caccagcgac aagccgctgt tgacgtcggc cgcgacgcgc gaacgcgtcc
     6721 tgggcggcac cgtgtcgcat gagctggccg atcgcatggc cgactcgccg ctgcctttcg
     6781 agtcggcccg cgtgcagcgc gggctgaact ggctggcgtt cggcgacctg ggaccgcgcg
     6841 tgcggcaggg ctgcccgggc tggctcttca tacgcgacga actgatggtg tatcgcgatg
     6901 ccagcgccca tgccgcgacg cgcgcccaga cggtcgctgc cgtgcagcgc gacctggcca
     6961 gccgcggcat caagctgctg gtcatcatcg ttcccgataa gtcgcgcatc gaagcgcagc
     7021 acctgtgcgg cctgcaccgc ccggcctcgc tggacggccg cgtcaacgac tggatggcgc
     7081 agttggccgc gcagggcgtg cgggccgtgg acctggcccc ggcgctgcgc ggcgtgcagg
     7141 gcgacgccta cttccacacc gacacgcact ggaccgaagc cggggcgggc gcggccgcgc
     7201 aagccgtggc gcagcgggtc caccaactgg gcctggcctt gcccccgggc ggcgcctaca
     7261 aggtcgaggc ccagcccgtg gcgcgccggc ccggcgacct ggtgcacctg gccggcctgg
     7321 actggctgcc gccgtccatg cagcccgaac ccgaaactgc ccaggcgcat acctatacgc
     7381 ccgaggccaa ggccgccgcg acggacgatg atctattcgg cgacgcggga ctgcccatcg
     7441 tgtccctgct gggcacctcg tattcgcgca attcgaattt cgcgccccag ctggcgatgg
     7501 cgctggggtc gggggtgaac aatttcgcgc gcgacggcgg catgttcggc ggcgcggcgc
     7561 aggcctattt ccccagccag gcctacaccc agacgccgcc gaagctgatc gtctgggaag
     7621 tggacgagcg cgacttgcag gcgccgctgc tgccggcgga tgccgtcgcg gttccccttg
     7681 cctcccctgc cgctccgggc gccgccggcg gcgcccgggc gtcccaataa cgcctgacca
     7741 tgaggtgctt tcatccatgg cgactcgacg caggtttcta accgcgctgc ctttgctggg
     7801 cagccccgtc ctggccggcg ccgcgacctc ccgggcctat gccgtcgtca tcggcgactc
     7861 catcgcggaa ggcgaaccca accggcacgg ccggctcaac gtgaacggcc agttcgttcc
     7921 cgactaccct tcgcaaccgg gccagctctc ctacgaactg gccaagtatt cgggcgtgtt
     7981 ccacgtcaat cacggcatag gcgggcaggt ggcggcccag gtgcgccagc gctggccgcg
     8041 cgacgtgctg gccgaggacc tcgaccccgg cgacggccgc ggttcgcgca cgctgccgaa
     8101 aggcaggctg ccggcgctgg tctatctgca cgtgggcatc aacgacatcg cggcccgcgt
     8161 gccattgcag tcgatgcagg acagcttcac ctattttgcc cagaccacgc aagggcgcgg
     8221 cctgccgctg gtcatcgaca acatcggttc ctggctgggc atgccgccgc ccatggtcga
     8281 cgaggcccgc gccttcaaca cctggctggt cgaggaactg gcgccccgcc accagaacat
     8341 cgtcgtggcc gactatctgg actggtccag cggcaacacg ggggattacc gcgtcatggc
     8401 cccaggcaag ttcgtcgacg gcctgcatcc caccatggag ggctaccagg acttcgcccg
     8461 ctacgtgcac gacacggtaa aactgccggc ccgcagcgcc gtgcactact gattgcgtgc
     8521 ggcccgctcg gccgcttcga ccgtgttgac cagcagcatg gcgatggtca tcggcccgac
     8581 gccgcccggc acgggcgtaa tggcgccggc caccgcggcg gcggagtcga agtccacgtc
     8641 gccgcacagc ttgccgtccg cgccgcggtt gatgcccacg tcgatcacca cggcgcccgg
     8701 cttgatcatg tcgccggtga ccatcttcgg cttgccggtg gcgaccacca gcacgtcggc
     8761 gcggcgcgcc tgcgcggcca ggtcgcgggt cttggagttg cagagggtga cggtggcgcc
     8821 cgcctgcagg agcagcatgg ccatgggctt gcccacgatg ttgctggcgc ccaccaccac
     8881 ggcctcggcg ccgcgcagcg ccgtgccttc ggactccagc atcttcatca cgccataggg
     8941 cgtgcagggg cgaaacagcg gctggccggt catgagcagg ccggcgttgc taacgtgaaa
     9001 gccgtccacg tccttttcgg cggcgatggc ctcgatgacc ttgtgcgcgt ccatgtgctt
     9061 gggcaggggc agctgcacca ggatgccgtg gatggccggg tcggcgttga gcgcgtcgat
     9121 gcgggccagc agttcggcct cgctcagggt ggcggggtac tgttccttga cggagtgcag
     9181 gccggcctgt tcgcaggcgg ccaccttgtt gcgcacgtag acctgcgagg cggggtcttc
     9241 gccgaccagc accacggcca ggccgggggt gatgcccttg gatttcaggg ccgtgacgcg
     9301 cagggcaact tcggcgcgga ctttttcgga tagggctttc ccatcgatga ggcgggcggt
     9361 cattgtgtct ttgatgtcaa tgttgagaag ggatgaggca gagtgcgggg gcctaagcgt
     9421 tcttaacccg ttcttcgtcc gtcgggcata ccccaggggc ttcgctgagc cgggttcggg
     9481 cgccgcctaa ggcgcccgaa gtccctgcgg gactgccccg actcagaggc gcccctgggg
     9541 tatgcccgac ggacgaagaa ctccggttga ggctgctccc aggccgtcgc atgcggtaca
     9601 tctgagcatc gccgccatgg gcgcaaagcg cgcaggcgcc tatgaattgg ggcagtcccg
     9661 aagggacttc ggccgcctta ggcggcggcc gaacccaatt catcgaagcc ggagcgcttt
     9721 gcgcccatgg cggcgatgcg gctaaagaac acaacactgc gaagaccagc caaaatcaag
     9781 catgcgcctc gggcttggcc agggcgacct tcatcaggtc ggccacggtg gtgacctcga
     9841 gcttttccat gatgttggcg cggtgcgcct cgacggtctt gatgctgatg cccaggtcgt
     9901 cggcgatctg cttgttcagg cggccggcga cgatgcgttc gagcacctgc tgttcgcgtg
     9961 cggtgaggcg ggccagcatg gcttcgtggt cgcgctgggc ctggaactgg ttgacgcgct
    10021 gcacggcctg ttcgagcatg cgggccacga tgtcgcgcag gtcggcctcg ttgaagggct
    10081 tctcgaggaa gtccaccgcg ccttttttca tggtcgacac ggccatgggc acatcgccgt
    10141 ggccggtgat gaagacgatg ggcagcggcg ccttgcgcgc gatcaactgc tcctggagtt
    10201 ccaggccgct catgccgggc atgcgcacgt cggcgatcag cacgccgatc aggttgggat
    10261 cgtattcgtc cagaaaagtc tcggcgctgg cgtagggacg cacccggtag ccgttcgctt
    10321 cgagcagcca gcgcagcgaa tcgcgcacgg cctcgtcgtc gtcaacgatg aagacggtac
    10381 tggacatggg gggggtgttc atgcaggcaa ctcctcggac tggtcattct gcatctgggt
    10441 tttcgggtcg acggtcggca gggtgaacct gaagatcgtg ccgccggacg ggtttggatc
    10501 cgcccagagg cggccatggt gggactcgat aatggtacgg caaatattca gtcccatgcc
    10561 caggccctgg gtcttggtgc tgaagaaggg ctcgaacagc cgctcgggct cggcgatgcc
    10621 gtggccgcgg tcgaccacgg ccacctccag gtgctgttcg tgcaggatga cggccacctt
    10681 gagctcgtcg gactcgctct tttccatggc ctccatgccg ttcttgatca ggttgagcag
    10741 cacctgttcg atgaggatgg ggtcggccag cacgtcgggg gcggtcgacg gcacgaagct
    10801 gtcgatgcgg atgcggcgct tgcgggcgtc gatctcggcg aagccgatgg cgttgtcgac
    10861 gatgcggcgg atgggcacgg cctggcggcg cggctcgctg cgtttgacga actcgcggat
    10921 gcggctgatg atcttgccgg cgcgctcggc ctgggcggcc gccttttcca gcgcgggcag
    10981 cagcatgttg gggctggtgt ggccggcctt gaccagggcc accgcgccca tgctgtagtt
    11041 ggagatggcc gtcagcggct ggttgagctc gtgcgccagc gacgaggcca tctcgcccat
    11101 ggtggtcagg cggctggtga tctggatctt ttcctgctgg acgcgcgagg cttcttcgtg
    11161 ctggcggcgc tcggtgatgt cgcgcgcgac ctgcatgcgc acgcggcggc cgtccgtcca
    11221 ggccagcatg cggtgatgca cctcgaacca gcgctgcacg gaaggcgcga acacttccac
    11281 cgattcgtcc gtgaaccgcc cccggcggcc ggccaggagt tcgtcgtggc cgcccgtctg
    11341 cgcgccgaag acgcgccggt aggtgcggtt ggcgaacagc agctccaggc cgtcgtgcgt
    11401 gtcggccgtc accgagatgg cgtcgtccag gccttcgagc acggtcatga agcgctcgtg
    11461 cgcggcggtc agggcttcgc ggatgcgctt gggctcggtg atgtcggtca tcgaggtcat
    11521 ccagccgatc tggttgccat tggggtcgag cagcggcgag acgtacatgc gcgcggcgaa
    11581 gcgcgagccg tcgcgccgct gcgcctcgat ttccaggccg ctgctgggcg tcttgcccga
    11641 catcagcacg tcgagcgtgt gctggtgctg gtcgtggcgg cccggcaccc aataggggaa
    11701 gggcggcgag cggccgatca ggtcggcctc gttccagcct atcatgcggc agaaggccgg
    11761 gttgacgtag gcgatgcggc cttccatgtc gagcacgcgc atgccggtgg acatggagtt
    11821 ttccatggcg cggcgaaagc cggtctcggc gatcagcgcg gcctcggcct cggaacggaa
    11881 gcgcgtatag cgccacagca tgagcaggct caccacgatc acgcacgaca gcgcgatcac
    11941 cacccacaag aggcgctgct ggcgcagctc ctggtcgatg gtgacgaaca tgacgctgtc
    12001 gtcgcggatg cgctgcagcc agaagaaaac gcccatcacg cacaggtaca gcaccagcac
    12061 gagcgccggc gtcagccagt acaggccgcg gcggcgcatg cgggcatcgt cgtgaaacgg
    12121 cgtagggatg tgagatttgg ggctggacat gaattcttgg caggaattcc gccattttag
    12181 tgtgtgtccg cgtcccgcat ggggcggccg accggatgta tgttcaatgt catctggatt
    12241 tcacattaca aaatgccgta ccgcaacatg aaattttccg cgtgacgagt taagcttccg
    12301 gcccagcaag ctaaagtcat gcccaggcga tacgccgggt gtggcgtcga accctattca
    12361 cggagacagc acatgtcctc ttctgttcag gctggtgccc ccgcggccgc caatgacgac
    12421 gataacctcg aaacccagga atggcttgaa gcgctggccg ctgtcctcga ccgtgaaggg
    12481 cccgaccgcg cgcacttcat catcgagcgc ctgatcgacc aggcgcgccg ttcgggcgtc
    12541 cacatcccgt actcgcccaa cacagcctac gtcaacacga ttcccaccgc gctcgaaccc
    12601 cggcatcccg gcaatctcga gctcgaggcg cgcatccgcg cctacatccg ctggaacgcc
    12661 atggccatgg tggtcaaggc caacaagcac aatccgcccg acggcggcga cctgggcggc
    12721 cacatcgcct ccttcgcctc gctggccacc atgatcgact gcggccagaa ccacttctgg
    12781 cacaccgaat cggccgagca cggcggcgac ctggtctact tccagggcca ctcctcgccc
    12841 ggcatgtacg cgcgcgcctt cctggaaggc cgcctgtccg aagaacagct cgacaacttc
    12901 cgccaggaag tcgagggcaa gggcctgtcg tcctacccgc atcccaagct gatgcccgag
    12961 ttctggcagt tccccacggt gtccatgggc ctgggcccgc tgatggccat ctaccaggcg
    13021 cgctttctca agtacatcca cgcgcgcggc atcgccgaca ccagcaagcg caaggtctgg
    13081 gtgttctgcg gcgacggcga aatggacgag cccgagtcgc tgggcgccat cgcgctcgcc
    13141 gcgcgcgaaa agctcgacaa cctggtcttc gtcatcaact gcaacctgca gcgcctggac
    13201 ggtccggtgc gcggcaacgg caagatcatc caggaactcg aaggcgactt ccgcggctcg
    13261 ggctggaacg tcatcaagct gatctggggc ggctactggg atccgctcct ggccgccgac
    13321 aaggaaggca ttttgcgccg cgtcatggaa gaaaccgtcg acggcgagta ccaggcgtac
    13381 aaggccaatg acggcaagtt cgtgcgcgag cacttcttcg gcaagaaccc caagctgctg
    13441 gagatggtcg cccgcatgag cgacgaagac gtctggcgcc tgaaccgcgg cggccacgat
    13501 ccgcacaagg tctacgcggc tttcgacgcg gcgttcaagc acaccggcca gcccaccgtc
    13561 atcctggcca agaccatcaa gggctacggc atgggccccg tgggccaggc caagaacccc
    13621 acccaccagc aaaagaagct ggagctggag tccatccgcg aattccgcga ccgtttcggc
    13681 attccgatcg ccgacgacga cctggccaag gtcccgtact tcaagccggc cgccgattcg
    13741 cctgaaatgc aatacatgca ggcgcagcgc aagacgctgg gcggctacct gcccaaacgc
    13801 cgcaccaagg ccgacgagca cctcatcgtg ccgggcctgg acgccttcaa ggccgtcctc
    13861 gaccccaccg ccgagggccg cgagatctcg accacccagg ctttcgtgcg cacg
//
LOCUS       DASWPO010000067        20900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1710688, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000067 DASWPO010000000
VERSION     DASWPO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20900
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710688"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VGC69_04120"
     CDS             complement(<1..486)
                     /locus_tag="VGC69_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HEY0294506.1"
                     /translation="MALATLLAQAWTAQAQTVRSADEAAAGEVRRFYAIAPGPLATAL
                     SQFASQSGVLLSVDARLTEGKQSPGLNGEASVDAGFQRLLVGSGLAARQGERGVYTLQ
                     ALPAGGVTELDAVTVVGSAVRADTYQPAPVAGILRSSTPVMEQAQAVNVVPAQVIRDQ
                     RP"
     gene            complement(640..1644)
                     /locus_tag="VGC69_04125"
     CDS             complement(640..1644)
                     /locus_tag="VGC69_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0294507.1"
                     /translation="MSDGVLPSLGSPEGEGLALSPSVLEAAAQWFATLGADTVDERDR
                     QRWQAWHDASVEHARAWRAVLAISQEFNAAAGMPGREVLSQAGRYRRRSLKRWAWLFG
                     ASLAGGGFAARPGARRYLAALGADLRTNVGEIRQFALSDGTRAWLNTDSAVNLHYSDS
                     LRLVELVAGEILIETAKDARQPARPFEVETPAGRMRALGTRFCVRLEGDRTRLDVFEG
                     SVAVRPSLGGESRIVPAGQGIGFDAAGYGDMETAEPASIAWQRHELVALNMPLRVFLA
                     ELGRYVHGYLGCDDAVATLRLVGVYPLDDPDRIFQALENSLPVRVRRPLPWWVRVEPA
                     "
     gene            complement(1641..2156)
                     /locus_tag="VGC69_04130"
     CDS             complement(1641..2156)
                     /locus_tag="VGC69_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0294508.1"
                     /translation="MAPNSSAGAVVHSLYAAHHSWLFGWLRHKLGCEQQAAELANDTF
                     VRLLASRQAEQLREPRAYLTRVAHGLVVNHWRRLTLERTYLDALAAQPEAVSPSPEQR
                     MLALEALCRLDAMLDSLGAKVREAFLLSQLDGLTYAQIAERLQVSERMVKKYMTQAML
                     QCLLVVGGAPP"
     gene            complement(2296..5865)
                     /locus_tag="VGC69_04135"
     CDS             complement(2296..5865)
                     /locus_tag="VGC69_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294509.1"
                     /translation="MYFDYRLWQLTLGMRTRMAGGIVLGLLALAAGIGRYVFLGQMLA
                     RVFADQPWQAWSAPAAWAVAMVLLRALLDHGRTRQANRCAATIQQVLRARLYDRIVSL
                     GPAWFANQRTGGVMLTVVDGVEQLQTFFGQYLPQVAIAAAAPFAIFAVIAYWDLPIAA
                     VLLAAALLALFGPMAVHRMDKRASLARAHSLQEFGEEFLDAVQGLPTLKSFGQGKSWG
                     RRLAERARRLSDSTVWVLSVTLLTRGISDLGVALGAALALTLGAWRVRHGEMSVEALL
                     IVLMAGTEIFRPLRDLRAVLHRGMLGQSAAAGIHALMEAESAASSHAGQARGLAPERL
                     DPTIEFDDVSFAYARERPAHRGLRFRIEAGERVGVVGPSGAGKSTIVRLLLKEVLPQA
                     GTIRVGGHDARDLDAQALLSRIAVVSQDITLFHGTLDENLRLGRPEATHEEVRAAARA
                     ANIDDFIVALPDGYATRIGERGLQLSGGQRQRVAIARALLRDAPILILDEALSSVDTE
                     NEAAIQQALDRLMAGRTTLILAHRLASILGADRCLVLDQGRVVEQGTHAELMVRQGLY
                     YRLMHEQDKARRQAGRAGAGDAAGTADGQDGEDARGAPRSAPAPRALDEDAGRVGWHA
                     TLATLFAVVRPWRGTLLATILLGVARVAAYIGVGVLSALVVAAIRGGRDPGGFVIALL
                     VAAPLAALFHWLESWLAHAMAYQLLADMRIKLYDKLERLAPAYLLQRRSGDLVALAAQ
                     DVEMIEYFYAHTIGPAIVSLLVPLTVLGFLAVYSWPVALALLPFLAYALVSPTRGRRR
                     IDALGDKAREALGEMSAYITDTIQGLAELTAFQATGRRRARFLAVAERYGRRRLDILH
                     DLSAQTAWFEVAMGLGGVAVAVVGAMQVSAGALEAGMLPLLILVALATFLPVSEISQV
                     SRQLADTIAATRRLHVVAHEPEPVADGPLPAPHAAHGLSLEFDHVSFAYPGKPGDALR
                     DLSFKVPAGATAAVVGASGAGKSTVASLLLRFWDPRQGQVRLGGVDVRQLQLDALREC
                     VALVTQDTYLFNGTLEANIRLARPGASDADLSAALEQAALSDFVRALPDGLATRVGER
                     GVQLSGGQRQRISIARAFLKNAPVLILDEATSHLDTLSELQVRQALAVLMQHRTTLVI
                     AHRLATIQDADLILVLQDGALAQSGTHEALLGQQGFYARASSH"
     gene            5969..6910
                     /locus_tag="VGC69_04140"
     CDS             5969..6910
                     /locus_tag="VGC69_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294510.1"
                     /translation="MATLPTRTPPLAALRAFEAVARLGSLSRAAAELNVTKSAVSHQL
                     RTLEADLGVVLLRRGGTTRRAQTTEAGTDLLASVQQALTLLSAACRNARAAARDRQHY
                     RLNISANPSIAALWLAPRIGRFIELHPDIEVQVYLHTNQDPAWKEQDIDLAFLHMRDE
                     GPHRPDPGDIRLMAESVVPVCSPALVDASERGDPAVFTRHRWLEEKHVASPETSWRTW
                     QKKLGLRDADRQEPLVLSGLSTVVSAAVAGVGIALGRMPLVDEEIARGRLVPLVPGLR
                     MAGSWGYVIRLRPDRPGDAALPALVEFLVEEAKQPAA"
     gene            complement(6907..7782)
                     /gene="copD"
                     /locus_tag="VGC69_04145"
     CDS             complement(6907..7782)
                     /gene="copD"
                     /locus_tag="VGC69_04145"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HEY0294511.1"
                     /translation="MWLTAATAALRFVSDAGLMFLFGSCGLLAATAGRDGPWQGGPSF
                     LRTRRLAAALALAALFGILAMQAASVGDGWPSALDPSMLRAVMLQTGFGHAWMARLVL
                     AALMSAVILASPRPGVAAMSAGLALAAASLTGHAAMHEGVLGIMQRLVQALHGLCAGA
                     WVGSLVPFFWLLRALRQPGRHAAASRALRRFSRLGHVAVAGVLLTGAANTAFILGRWP
                     VNWHSPYQALLAAKIGLTLLMVVLAIMNRYVWAPRFSHAPDATAEAIRLGTLGELAAA
                     VLALALVCVLGLLEP"
     gene            complement(7782..8147)
                     /gene="copC"
                     /locus_tag="VGC69_04150"
     CDS             complement(7782..8147)
                     /gene="copC"
                     /locus_tag="VGC69_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HEY0294512.1"
                     /translation="MKYLARVGLACLLGLGYVASASAHAHLKAAEPGQNASVAAPNAL
                     SLHFTEGLELAFSGVSVTGPGQKAVPLGSAHTAAGDDATLVVPLSQPLVPGVYTVDWH
                     ALAKDGHKTRGSYAFTVTQ"
     gene            complement(8272..9069)
                     /locus_tag="VGC69_04155"
     CDS             complement(8272..9069)
                     /locus_tag="VGC69_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0294513.1"
                     /translation="MSQARLQTFGELPGAHKVIAQSIDYEDGTHEIAHVHYRSQLVYA
                     TTGIVRAVTPLGLWMLTPGQALLIGSQIEHELHMVGKVSMRTLYIDPGVFAEDGVECR
                     VIDVEPLLRESIRAMFDPALSDGEDAENTRETLLVPLILRLLRLACARAANARLPLPR
                     HARLRRICERLIDQPASNDTLDDWADEIGASARTVARLFRKETGMTFGQWREQLRLTE
                     AMSKLAVGQPLAHIAQQLGYADVRTFSVMFRRAFGSTPQQFRQHLGK"
     gene            9387..11570
                     /locus_tag="VGC69_04160"
     CDS             9387..11570
                     /locus_tag="VGC69_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0294514.1"
                     /translation="MALLLYLLGGVATALAQSSDTTSASGTAATGTSPTAPVSQLPAV
                     EVLGEHEQSDTGSSDRYVPISTITATKTDTPLIETPQSVSVVTRQQMTDQNVQSVTEA
                     LRYTASVLSEVRGASAAGAPYLMSRGFYLEQFLDGSRLPSNVNFGYAIPSFDPYGLER
                     IDVLHGPSSVLYGQASPGGVVNMVSKQPTTTPVHDMFITTGNHNRAEAGFDLGGALTS
                     DGTLSYRLTGSADQNDTQVDGVKQKHVYIAPSITWKPTENDTLTILAKYQRDPDVGYY
                     NFVPAYGSLFDTPNGKISSHTNLGDANFDSHSKTQASIGYTYEHRFDPTWTFRQNLRY
                     TYVSDDFSNVFANGFAPGSSTTLNRYAFFNDESARFFTLDNQVQAKFSTGDISHTALF
                     GADYQRVLYQERVGLGLAPSLNPFDPVYASVAMPATTSDDFIRMKQIGLYAQDQLAWN
                     KWRFLIGGREDFASADDNNPVTSSYTSQSQKAFTWRTGLVYLFDNGIAPYASYSTSFN
                     PQAGVLADGSVTKPTTAQQYEVGVKYQPPGLNSYITAAAFDLHERNVLTADLLNPGYS
                     DQAGKVRARGVELEAHANLSRDLNLTASYAYLSSVTTESNDTGTALDGSSVSLQGKQT
                     WGTPRNILAAWLDYTRHGGPLNGLGFGAGVRYLGASYDTTNTIRVPSATFIDAAVHYE
                     VDRHWLLSLNASNLLNRKYVASCYSAATCTYGSGVEVLASARYRW"
     gene            11588..12439
                     /locus_tag="VGC69_04165"
     CDS             11588..12439
                     /locus_tag="VGC69_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294515.1"
                     /translation="MQAADTPDLFHPERTRSEHARAARPAGPLYEIDGVSFEIDARVL
                     LQPLTLTLHAGRMIALIGHNGSGKSTLLKLLARQQPPTRGTLRFAGTGLGAWPAREFA
                     RRVAYLPQHPPAGTHMTVRELVALGRYPWHGALGRVTDEDRAMVERALQMTDTARFAT
                     QLADHLSGGERQRVWLAMLVAQGASCLLLDEPTSALDIAHQIEVLRLLRRLTDEHGLT
                     VVIVLHDINLAARFSDELIALHTGRLLTAGPPAALMQAHVLESIYGVPMDIVQHPHGG
                     GPLAMAR"
     gene            12441..13361
                     /locus_tag="VGC69_04170"
     CDS             12441..13361
                     /locus_tag="VGC69_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294516.1"
                     /translation="MRRRTLLRAAGMMALCGVRAAAGAPPAMAGRAAPRIAVLDWGLA
                     ETLLALGVVPAGVAEIAGYNANVVTPAVPLDVPDVGLRLAPSLEWLAQLAPDLILVNS
                     SQESQREMLERIAPVRAFAVYTDAGTPYRLAQQATRELGTLCRCETAAEALIARTAQA
                     LDAGRARLAAASAHSRPLYLIRFFDSRHIGVYGAHSLFQDALDALGLANAWHGATDYW
                     GIGVAPLEALAAAPDATVIYFEPLPQDAGHTLAANRLWRALPAVAAGRIAALPPFWGF
                     GMLPSVQRFGAALAQALVATPGAMQGNAGS"
     gene            13358..15412
                     /gene="fhuB"
                     /locus_tag="VGC69_04175"
     CDS             13358..15412
                     /gene="fhuB"
                     /locus_tag="VGC69_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HEY0294517.1"
                     /translation="MSGLHRFASPAARAAFAPPGAPGSPVAVFMVIALALAVALGLSA
                     ANLAAVAPRALWWQAICSPDAHDVRQIIVHDSWLPRIMAAWLAGATLGLAGTLFQQVL
                     RNPLAEPMTLGVSAGAQLALTLAAIWAPAVPAFAREPVALAGALGAAVAVFGLAWRSG
                     LAPVTVAVAGLVVTLWCGSIGVALQIFHEPYMHGLFIWGGGSLVQQDWRDVATLAPRL
                     AVGGTLAWLLRRPMTLLGLDEANARSLGLGLRRLRLATLALAVALSAAVVSAVGVIGF
                     VGLAAPAIAQLAGARRLSQRLVMAPLCGSLLLWLTDQAVQPLAHALGELVPAGAASAL
                     FGAPIMLWVLAHMPRAQALDSSAGPAFHAVPGVSISRRFVLIASAAVVMAVVSLDLGR
                     GPDGWHFSGLDALSHIAVWRMPREFAALAAGIMLALSGTLMQRLTGNPLASPEVLGVS
                     AGAALGFIALVLGRAEASHGAQLAATALGAFATLAAILWFAKRSGFAPQRVLLVGIAI
                     GSLFQAVVAVVIAGGGERAAILLRWLSGSTYTVTPADATLALVLCAVLGALMPLTARL
                     LDILPLGEASARALGMRTALARAALLGLTALLTAAATLIVGPLSFIGLTAPHFARLAG
                     ARRPLAQILLAAPIGGMIMVAADWLGRTLLMPRELPAGLVATLLGAPWLMWLLARRRG
                     GA"
     gene            15461..17167
                     /locus_tag="VGC69_04180"
     CDS             15461..17167
                     /locus_tag="VGC69_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HEY0294518.1"
                     /translation="MIAELLRLLRRLWPISALATLMGISSGVATAALLAVTNHALHDG
                     AGSLPTLLASFVGLCVLALAGEVISDIGNNFVGQRVIATLRKNLCEKIIAAPISQIEQ
                     FRSHRLITALNQDIDVISTFSFMLSSLAIATATIAGCLGYLFVMSPLMLLIALCAIAL
                     GAIVQSLARRAGIRRFGEAREADDHLQKHYRAMVDGAKELRINRERRAFVYRHELSGT
                     IDAIFRMRVRAANVFVTANAFGSLLFFIVVGLMLVLQTGAADADRTVITGFVLVLLYM
                     KGPIQEIIGALPSIGRAQVAFRRITELSADFSSSEPALLQADATASERAASFTSLALL
                     DVAYRFSAPQDDSLAATQPDAGGFALGPLSLTIERGETLFIVGENGCGKTTLIKLLLG
                     LYTPGAGALLVDGAPVREDTLDDYRQRFSVVFSDYYLFDTLLPGASAIGAEAQGYLER
                     LALAHKVSVRNGAFTTTDLSTGQRKRLALVHAYLERRPVMVLDEWAADQDPMFRRIFY
                     TEILPDLKRQGKTLIVISHDDRYFHVADRVIRLEQGKIIQEASGRMFTYEDRGAAPPR
                     DQ"
     gene            complement(17221..17907)
                     /locus_tag="VGC69_04185"
     CDS             complement(17221..17907)
                     /locus_tag="VGC69_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY0294519.1"
                     /translation="MLITLPDVLTPDAAAQMRRRLETAGDAWVDGRATAGYSGAPVKD
                     NQQIAEGSPVARELGDAVLASLERNPRFISAVLPNKVYPPLFNRYQGGQHFGGHVDGA
                     IRLIPGHGARVRTDVSITLFLTPPEDYDGGELVVEDAYGTHEAKLPAGHAVVYPATSV
                     HHVRPVTRGVRLGCFFWVQSLVRTDAQRALLFDMDNAIQRLNASGADDVARRTLVGCY
                     HNLLREFSDT"
     gene            complement(17913..20405)
                     /locus_tag="VGC69_04190"
     CDS             complement(17913..20405)
                     /locus_tag="VGC69_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0294520.1"
                     /translation="MLASPRVSSSARAPRRTFRLAPLATALQGAMMLCLTLGAIAPVR
                     SALAQTAAAPAKAAMRRFDIAAGPLQPALDRYAQQAGINVSYDPAVIGSATTAGLHGE
                     YEAGAGLQALLAGSGLQAARSGGGYVLSRPSAADTGGVAALPTVTVEADSLGNDFQAP
                     LSSVGGRFTMDQHDIAQATAVVNKALLQSQAASSFQDALRNVPGVTIGAAEGGVIGNN
                     INLRGFTANNDIYLDGFRDRGQYYRDTFDLESIDVLYGPSSLYFGRGSTGGAINQVSK
                     QANLKPSAEVSTTIGTHDRERGTFDVNQPLSDTSALRIDGFGQSLSSTRDVMQNQDFG
                     IAPTLRLGIGTPTEITLSALVQHNDDQPDYGVQSLNGRPAPVSPSTFYGFTDDRTIQD
                     VQAFTARIEHTFNSNVKLRNQTRFGHYSTDARETSLKRGLYTGPTASSPALGAGDFTT
                     LPPSQLFVGLVSRDRVINNHSIYNSTDLEAKFGSGWLKQDVVAGMDIEHDSYSNQGYR
                     DTAPGLPLGTLAIVPLLDPPQTTRPANAVQTLDDLAQSSANSFGAYINDTVSLGEHWK
                     AVGGLRWDRFSASISNSTGTPAHADNTNNFTSVRTGLIYQPTEAQSYYVSYGTSFDPS
                     LEALTLTSGQENLPPETNRSYEVGSRWDLLDGNLSVAQSLFQIEKSNARTQDADGDFT
                     LNGDVRVKGYQIGVAGRLTKAWQVYGGYTYLDSRVVKALDGTQGNALPNTPRHTVTLW
                     TTYAITPHWQIGGGPVYLSSRYAANDNIVKVPGYVRWDAMAAYHADRYDVQFNVINLT
                     NKAYYDALIPSDGGRAVPALGRTFLVTLDYKF"
     gene            complement(20536..>20900)
                     /locus_tag="VGC69_04195"
     CDS             complement(20536..>20900)
                     /locus_tag="VGC69_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron dicitrate transport regulator FecR"
                     /protein_id="HEY0294521.1"
                     /translation="DGMAELYLASGMRAGIVRRGEYWRLAADGASQLAQPDFDVTSWA
                     DGSINAHRMRLADLLAELARYRVGHIACDPSVADLRLTGVYQLDDIDQTLRFIAQTQP
                     VRIVRRTPLWLSVVPAGA"
BASE COUNT         3380 a         7316 c         6894 g         3310 t
ORIGIN      
        1 cggccgctgg tcgcgaatga cctgggccgg cacgacgttg accgcctggg cctgctccat
       61 cacaggcgtg ctggaacgca ggatgccggc caccggcgcg ggctggtagg tgtcggcccg
      121 taccgcgctg cccaccacgg tgacggcgtc gagctcggtg acgccccccg cgggcagcgc
      181 ctgcagggtg tagacgccgc gctcgccctg gcgcgccgcc aggccgctgc ccacgagcag
      241 gcgctggaac ccggcatcca ccgaggcctc gccgttcaag ccggggctct gcttgccctc
      301 ggtcaggcgg gcgtccaccg acagcagcac gcctgactga ctggcaaatt ggctcagggc
      361 cgttgccagc ggtccgggcg cgatggcata gaaccggcgg acctcgccgg cggcggcttc
      421 gtccgccgat cggacggtct gcgcctgtgc ggtccaagcc tgcgccaaca gagtggccag
      481 cgccaacggc gccaagcgcc tggatctggc gcccagggat cgaacaaggg tcttcagtgg
      541 catgcgtata cccgtatgga tgcggtaggg atgaaaattc ttccgttacc tgcattgcca
      601 aacgagcaag caaaaaggga accgccgtta caaaaatctt tacgccggct ccacgcgcac
      661 ccaccacggc aggggacgcc gcacccgcac cggcaagctg ttttccagcg cctggaatat
      721 gcggtccggg tcgtccaggg gatagacgcc gaccagccgc aaagttgcca ccgcatcgtc
      781 gcagcccagg tagccgtgga catagcggcc cagttcggca agaaacaccc gaaggggcat
      841 gttgagagcg accagttcgt ggcgctgcca ggcgatgctg gccggctccg ccgtttccat
      901 gtcgccgtag cccgccgcgt cgaagccgat accctgcccg gccggcacga tgcgcgattc
      961 gcccccgaga gagggccgta ccgccacgga gccttcgaag acgtcgagcc gcgtgcggtc
     1021 gccctccagc cggacgcaaa accgcgtgcc cagcgcgcgc atgcgtcccg ccggcgtctc
     1081 gacctcgaag ggccgcgccg gctgccgggc atccttggcc gtttcgatca ggatttcgcc
     1141 ggccacgagc tcgaccagcc gcaggctgtc gctgtaatgc agattgacgg cgctatccgt
     1201 gttcagccag gcgcgcgtgc cgtcgctgag cgcgaactgc cggatctcgc ccacgttcgt
     1261 gcgcagatcc gcccccaggg cggcgagata gcggcgcgcg ccaggccggg ccgcaaagcc
     1321 acctcccgcc agagacgcgc cgaacagcca ggcccaccgt ttcaggctgc gccggcgata
     1381 gcggccggcc tgggacagga cctcgcgccc gggcatgccg gcggcggcat tgaactcctg
     1441 gctgatggcc aggacggcgc gccaagcccg cgcatgctct accgaagcat cgtgccaggc
     1501 ctgccaacgc tgccgatcgc gctcgtcgac tgtgtcggcg cccagcgtgg cgaaccactg
     1561 cgcagccgct tccagcactg acggagagag ggcaagccct tcgccctctg gcgagccgag
     1621 ggagggcagc accccgtcgc tcatggcggc gcgccgccca ccacgagcag gcactgcagc
     1681 atggcctgcg tcatgtactt cttgaccatg cgttccgaca cctgcaggcg ctcggcgatt
     1741 tgcgcgtatg tcaggccgtc cagctgggac agcaggaaag cctcgcgcac tttggcgccc
     1801 aggctgtcga gcatggcgtc gagccggcag agcgcttcga gcgccagcat gcgctgctcg
     1861 ggcgagggcg atacggcttc gggctgcgcg gccaaggcat ccagataggt gcgctccagg
     1921 gtcagccggc gccagtggtt gacgaccagc ccatgcgcga cccgggtcag gtaggcgcgc
     1981 ggctcgcgca gttgctcggc ctgccgggag gccagcaggc gcacaaaggt gtcattggcc
     2041 agctcggccg cctgctgctc gcacccgagc ttgtgccgca gccaaccgaa caaccaggag
     2101 tgatgcgcgg catagagcga gtgaaccact gcgcccgcgg aggaattggg ggccatcacg
     2161 gcaagacctg gagtgctcgc aatagatatt aagaactaat ctcattatcc atttcctggc
     2221 aattcctgca agtcgccttt tccctgccga tacgatgccg cccttgccgc acgggaatgt
     2281 ccggccagtt cgatttcagt gcgagctggc gcgcgcatag aaaccctgct ggcccaacaa
     2341 cgcctcgtgg gtgcccgatt gcgccaaggc gccatcctgc agcaccagga tcaggtccgc
     2401 gtcctggatg gtggcaagcc ggtgcgcgat caccagcgtg gtgcggtgct gcatcaagac
     2461 cgccagcgcc tgccgcacct gcagttccga cagcgtgtcc aggtgggaag tggcttcgtc
     2521 caggatcagc acgggcgcgt tcttcaggaa cgcgcgcgcg atggagatgc gctggcgctg
     2581 tccgccggaa agctgcacgc cgcgctcgcc cacccgcgtc gccagcccgt cgggcagcgc
     2641 gcggacgaag tccgacaggg cggcctgttc gagcgccgcg ctcaggtcgg cgtcgctggc
     2701 gccggggcgc gccagccgga tgttcgcttc cagcgtgccg ttgaacagat aggtgtcctg
     2761 cgtcaccagc gccacgcatt cgcgcagcgc gtccagctgc aactggcgca cgtcgacgcc
     2821 gccgagccgg acctggccct ggcgtggatc ccagaagcgc agcaagaggc tggccacggt
     2881 gctcttgccc gcgccggacg cgccgaccac ggcggccgtg gcgccggccg gcaccttgaa
     2941 gctgaggtcc cgcagggcat cgccgggctt gccgggatag gcgaagctga cgtgatcgaa
     3001 ctccagggac aagccatgcg ccgcgtgcgg cgcgggcagc ggcccgtcgg cgaccggttc
     3061 gggttcgtgg gccacgacgt gcaggcgccg ggtggcggca atggtgtcgg ccagttggcg
     3121 gctgacctgc gagatttccg ataccggcag gaaagtggcc agcgcgacca ggatcagcag
     3181 cggcagcatg cccgcttcca acgctccggc cgaaacctgc atcgccccca ccaccgcgac
     3241 cgccactccg cccagcccca tcgccacctc gaaccaggcg gtctgtgccg acaggtcgtg
     3301 caggatgtcc agccggcgcc ggccgtagcg ctcggcgacc gccaggaatc gcgcccgccg
     3361 gcgccccgtg gcctggaatg ccgtgagctc ggccaagccc tggatcgtgt cggtgatgta
     3421 ggcgctcatc tcgccgaggg cctcgcgcgc cttgtcgccc agcgcatcga tgcggcggcg
     3481 gccgcgcgtg ggcgaaacca gggcgtaggc caggaaaggc aatagcgcca atgccacggg
     3541 ccagctatag acggccagga agcccagcac cgtcaagggg accagcagcg acacgatggc
     3601 cggcccgatg gtgtgggcat agaagtattc gatcatctcc acgtcctgcg ccgccagcgc
     3661 gaccaggtcg ccggaccggc gctgcagcag gtaggcggga gccagccgtt ccaacttgtc
     3721 gtagagcttg atacgcatgt cggccagcaa ttggtaggcc atggcgtgcg ccagccagga
     3781 ctccagccaa tggaagagcg cggccagcgg cgcggcgacc agcagcgcga tgacgaagcc
     3841 gccgggatcc cggccgccgc ggatcgccgc gaccaccagc gcgctcagca cgcccacgcc
     3901 gatgtaggcg gccacgcgcg cgacgcccag caggatggtc gccagcagcg tgccgcgcca
     3961 gggacgcacc acggcgaaca aggtcgccag cgtcgcgtgc cagcccacgc ggccggcatc
     4021 ctcgtcgagc gcgcgtggcg cgggcgcgga acgcggcgca ccgcgcgcgt cttccccgtc
     4081 ctggccatcg gccgtgcccg ccgcatcccc agcccctgcc cggcccgcct gcctgcgcgc
     4141 cttgtcctgc tcgtgcatca accggtagta caggccctgg cgcaccatga gttcggcatg
     4201 cgtgccctgc tccaccacgc ggccctggtc gagcaccagg cagcggtcgg cgccaaggat
     4261 gctggccagc cgatgggcca ggatcagggt ggtgcgtccg gccatcaggc gatccagggc
     4321 ctgctggatc gcggcctcgt tttcggtgtc gacggacgag agcgcctcgt ccaggatcag
     4381 gatgggcgca tcgcgcagca gggcgcgcgc gatcgccacc cgctggcgct ggccgcccga
     4441 aagctgcagc ccgcgttcgc cgatgcgcgt ggcatagccg tcgggcagcg ccacgatgaa
     4501 gtcgtcgata ttggccgccc gcgccgccgc gcggacttct tcatgggtgg cctcgggccg
     4561 gcccaggcgc aggttttcgt cgagcgtgcc gtgaaacagc gtgatgtcct ggctgaccac
     4621 cgcaatgcgc gaaagcagcg cctgcgcgtc caggtcgcgc gcatcgtgcc cgccgacccg
     4681 gatggttccg gcctgcggca ggacttcttt caggagcagc cgcacgatgg tggacttccc
     4741 ggcgccgctg gggccgacta cgcccacccg ttctccggct tcgatgcgaa agcgcaatcc
     4801 ccggtgcgcc gggcgctccc gcgcgtaggc gaagctcacg tcgtcgaatt caatggtggg
     4861 gtccaggcgt tcgggggcaa gccccctggc ctgtccggca tgcgaggatg ccgccgattc
     4921 agcctccatc agcgcgtgga tgccggccgc ggccgattgc cccagcatgc cccgatgcag
     4981 gacggcgcgc aggtcgcgca gcggccggaa gatttccgtg cccgccatca gcacgatcag
     5041 caaggcctcg acgctcattt cgccgtggcg cacgcgccag gcgcccaacg tcagggcgag
     5101 cgcggcgccc agggcgacgc ccaggtcgct gatgccccgc gtcagcaggg tgaccgacag
     5161 aacccatacc gtgctgtccg acaggcgccg ggcacgctcg gccaggcgcc gtccccagga
     5221 cttgccctgg ccgaaggact tgagcgtggg cagcccctgc acggcgtcca ggaattcttc
     5281 gccgaattcc tgcagggagt gggcccgggc caggctggcc cgcttgtcca tcctatgcac
     5341 ggccatcgga ccgaacaggg ccagcaacgc cgccgccagc aataccgcag cgatgggcag
     5401 atcccagtac gcgatgaccg caaaaatcgc gaagggggcc gctgccgcga tcgcgacctg
     5461 cggcagatac tgcccgaaga aagtctggag ctgttcgacg ccgtcgacca cggtcagcat
     5521 cacgccgccg gtgcgctggt tggcgaacca cgcgggcccc agcgacacga tgcggtcata
     5581 cagcctggcg cgcagtacct gctggatcgt ggcggcgcag cggttcgcct gcctcgtccg
     5641 cccatggtcg agcaaggccc gcagcagcac catcgcgaca gcccaggcgg ccggcgccga
     5701 ccaggcctgc cacggctggt ccgcgaaaac gcgggccagc atctggccca ggaaaacata
     5761 gcgcccgatg cccgcggcca atgccagcag gccgagcacg atgccgccgg ccatgcgggt
     5821 tcgcatgccc agcgtgagtt gccacaaccg gtagtcgaaa tacatcggga tcacccattc
     5881 atggttttgc gttccggtat tgtggttcgt gccgcgatag cccggatagt gatagtttct
     5941 cgatcaagag ttgagataaa ttacacgcat ggcaacgctg cccacccgta ctcccccgct
     6001 tgccgcgctg cgcgccttcg aggcggtcgc ccgcctgggc agcctcagcc gtgccgcggc
     6061 ggagctgaac gtgaccaaaa gcgccgtaag ccaccagttg cgcaccctgg aggccgacct
     6121 gggcgtggtc ttgctgcggc ggggcggcac tacgcggcgg gcgcagacca ccgaagccgg
     6181 cacagacctg ctggcttccg tgcaacaggc attgaccctg ctatcggccg cctgccgcaa
     6241 tgcgcgcgcg gcggccaggg accggcagca ctaccgcctg aacatctcgg ccaatccgtc
     6301 gatcgccgcc ctgtggcttg cgccgcgcat cggccggttc atcgaattgc atccggacat
     6361 cgaggtgcag gtctatctgc acaccaacca ggacccggcc tggaaagaac aggacatcga
     6421 cctggcgttc ctgcacatgc gcgacgaagg gccgcatcgc cccgaccccg gcgacatccg
     6481 gttgatggcg gaaagcgtcg tgccggtatg cagcccggcg ctggtcgatg caagcgaacg
     6541 cggcgacccc gcggttttca cgcgccatcg ctggctggaa gaaaaacacg tggccagccc
     6601 ggagacgagt tggcggacat ggcagaaaaa gcttggcctg cgggatgcgg accggcagga
     6661 gcccctggtc ctgagcggct tgagcaccgt ggtctcggcg gcggtggccg gcgtgggaat
     6721 cgcgctgggc cgcatgcccc tggtggacga ggaaatcgca cgcggcaggc tggttcccct
     6781 ggtgcccggc ttgcgcatgg cgggctcctg gggctacgtc atacggctgc ggcccgatcg
     6841 tcccggcgat gcggccttgc ccgctttggt cgagttcctg gtcgaagagg caaagcagcc
     6901 ggccgcctag ggttcgagca agcccaggac gcaaaccaat gccagcgcaa gcaccgcggc
     6961 agccagctcg cccagggtac cgagccgtat ggcctcggcg gtcgcgtcag gggcgtggga
     7021 gaacctgggt gcccagacgt agcgattcat gatcgcgagc acgaccatca gcagcgtcaa
     7081 tccgatcttg gccgccagca gcgcctgata gggcgaatgc caattcaccg gccatcggcc
     7141 gaggatgaag gccgtattgg cggcgcctgt caacagcacg ccggccaccg ccacatgccc
     7201 caggcgggaa aagcggcgca gcgcccgact cgctgccgca tgccgacccg gctgcctcaa
     7261 cgcccgcagc aaccagaaaa acggaaccag ggagcccacc cacgcccccg cgcacagccc
     7321 atgcagcgcc tgaaccaggc gctgcatgat gcccagcacg ccctcgtgca tggccgcgtg
     7381 gccggtcagg ctggcagccg caagggccag gcccgcgctc atggccgcga cgcccgggcg
     7441 cgggcttgcc aggatgacgg ccgacatgag cgccgcgagg accaggcgcg ccatccaggc
     7501 atgcccgaag ccggtctgca gcatcaccgc ccgcagcatg gacgggtcga gggccgatgg
     7561 ccagccgtcg cccaccgacg ccgcctgcat ggcaaggatg ccgaacaacg cagccagcgc
     7621 cagggccgcg gccagcctgc gggtccgcag gaaagacggc ccgccttgcc atgggccgtc
     7681 ccgccctgcc gtcgcggcaa gcaggccgca gctgccgaac aggaacatca gcccggcatc
     7741 cgacacgaat cgcagggccg ccgtggcggc cgtaagccac attactgggt aacggtgaag
     7801 gcgtaactgc cgcgggtctt gtggccgtct ttcgccaagg catgccaatc gaccgtgtac
     7861 acgcccggca ccagcggctg cgagagcggg acgacgagcg tcgcatcatc gcccgcggcg
     7921 gtatgggcgc tgccgagcgg gaccgccttc tggccaggcc cggtcaccga aacgccggaa
     7981 aatgccagtt ccagcccttc ggtaaagtgc aatgacaagg cattgggggc ggctaccgag
     8041 gcgttctggc cgggttcggc agccttcaag tgggcatgcg ccgatgccga cgccacgtaa
     8101 ccgaggccga gcaagcaggc cagcccaacg cgcgcaagat atttcattcc agattcgctt
     8161 tgaaattaga ggaagaccct gatgccgtac tttacgccaa tgaccaggcg gccccggtcc
     8221 cgatagggcg ccgtcaggcg ccaggactgc ctcagacctg cctggcctgg gttacttgcc
     8281 caggtgctgc cggaattgct ggggcgtgct gccgaacgcc cggcggaaca tcaccgagaa
     8341 cgtccgcacg tccgcgtagc cgagttgctg cgcgatgtgc gcgagcggct gtccgaccgc
     8401 gagtttcgac atcgcctcgg tcaggcgcag ctgctcgcgc cactgaccga aggtcatgcc
     8461 ggtttccttg cgaaacagcc gcgcgacggt gcgggcgctc gcgccgatct cgtcggccca
     8521 gtcatcgagc gtatcgttgc tggccggctg atcgatcagc cgttcgcaga tgcgccgcag
     8581 cctggcatgg cgcggcaatg gcagccgcgc gttggccgcg cgcgcgcacg ccagccgcaa
     8641 aagccgcagg atcagcggca ccagcagggt ctcgcgcgtg ttttcggcgt cctcgccgtc
     8701 gctgagcgcg ggatcgaaca tcgcgcgaat cgactcgcgc agcagcggct ccacgtcgat
     8761 cacccggcat tcgacgccat cctcggcaaa tacgccggga tcgatataca gggtgcgcat
     8821 cgacaccttc cccaccatgt gcagttcgtg ttcgatctgc gagccgatca gcaacgcctg
     8881 gcccggcgtc agcatccaca ggccgagagg cgtcaccgcg cgcacgatgc ccgtcgtcgc
     8941 gtacacgagc tgcgaccggt aatggacgtg ggcgatctcg tgcgttccgt cttcgtaatc
     9001 gatcgactgg gcaatcacct tgtgtgcgcc gggaagctca ccgaaggtct gcaatctagc
     9061 ttggctcatg tccttattga acgattttgc ataaaaattt cataataaag gccaaaattg
     9121 gccagtccag tgcctaaatt gtccggacaa tgccttattg gcccacctat agtatcgcct
     9181 gaatggcctg gattgcggcg aaagcagcgg caaggccgtc cgctccggtc ggcgtcttcc
     9241 ccccatcccg ttctgaattc atcgagaacc acgcaaagtt cgccgttttg tcgactttat
     9301 attgattaag aatcattgcc atttgcatta atattcatta cgatggaagg aatcgcatga
     9361 agcccagggt tattagcacg acggcaatgg cgttgctgct ttatttgctg ggcggcgtcg
     9421 ccaccgctct ggcacagtca agcgacacca cgtccgcctc aggcaccgcg gcaacgggta
     9481 cctcgcccac ggcaccggtc agccagctac ccgccgtcga ggtactcgga gagcatgaac
     9541 agagtgacac gggctcttct gaccgttatg tgccaatatc taccattact gccactaaaa
     9601 cagacactcc gctgattgag acgccccaat cggtctcggt cgtcacgcga cagcagatga
     9661 ccgaccagaa cgtgcagagc gtgaccgagg cgctgcgcta cacggcgagc gttctttccg
     9721 aggtccgggg cgcgtcggcg gccggcgcgc cctacctgat gagccgcggc ttctatctcg
     9781 aacagttcct cgacggctcg cgcctgccga gcaacgtgaa cttcggctac gcgatcccga
     9841 gtttcgaccc gtatgggctc gagcgcatcg acgtgctgca cggcccctcg tcggtgctct
     9901 atggccaggc cagtcccggc ggcgtcgtca acatggtcag caagcagccg accaccacgc
     9961 ccgtgcacga catgttcatc acgaccggca accacaaccg ggccgaagcc ggcttcgacc
    10021 tgggcggtgc gctgacctcg gacggcacgc tctcataccg gctgaccggc tcggccgacc
    10081 agaacgacac gcaggttgac ggcgtgaagc agaagcacgt ctacatcgcg ccctccatta
    10141 cctggaagcc aaccgagaac gacacgctga cgatcctcgc gaagtaccag cgcgatcccg
    10201 acgtgggcta ctacaacttt gttcccgcat acggcagcct gttcgacacg cccaacggca
    10261 agatctcatc gcataccaat ctcggcgatg cgaatttcga cagccactcg aagacccagg
    10321 cctcgatcgg ctacacctac gagcatcgtt tcgatccgac ctggaccttc cgccagaacc
    10381 tgcgctacac ctacgtgagc gacgatttct cgaacgtgtt cgcgaacggc ttcgcgcccg
    10441 gatccagcac cacgctgaac cgctatgcgt tcttcaacga cgaaagcgcg aggttcttca
    10501 ccctcgacaa ccaggtccag gccaaattct cgacgggaga tatctcgcac acggcgctgt
    10561 tcggcgccga ctatcagcgc gtgctctatc aagagcgcgt gggactcggt ctcgcgccct
    10621 ctctcaaccc cttcgatccg gtgtatgcgt ctgtcgcgat gccggccacc acctccgacg
    10681 acttcatccg catgaagcag atcggcttgt acgcgcagga ccagctcgcg tggaacaagt
    10741 ggcgctttct gatcggcggg cgcgaggatt ttgcctcggc cgacgacaac aatccggtca
    10801 cgtcttccta tacttcgcaa tcccagaagg ccttcacttg gcgcaccggt ctcgtctacc
    10861 tgttcgacaa cggcatcgca ccgtacgcca gctattcgac gtcgttcaac ccgcaggccg
    10921 gcgttctcgc cgacggatcg gtcaccaagc cgaccaccgc gcaacaatac gaagtgggcg
    10981 tgaagtacca gccgccgggc ttgaacagct acatcaccgc ggcggcattc gacctgcacg
    11041 agcgcaacgt gctcacggcg gatctcctca atccaggcta cagcgaccag gcaggcaagg
    11101 tgcgcgcgcg aggcgtcgag ctcgaagccc atgcgaacct gtcccgcgac ctgaatctca
    11161 ccgcctcgta tgcctacctg agcagcgtca cgaccgagtc gaacgacaca ggcacggcgc
    11221 tcgacggcag ctcggtctcg ctgcagggca agcagacatg gggcaccccg cgcaacatcc
    11281 tggcggcgtg gctcgattac acccggcacg gcggcccgct gaacggcctg ggcttcggcg
    11341 ccggcgtccg ctacctgggt gcgagctacg acacgacgaa cacgatccgc gtgccctcgg
    11401 ccacgttcat cgacgcggca gtccactacg aggtggaccg ccactggctg ctctcgctga
    11461 atgcgagcaa cctgctcaac cgaaaatacg tggcctcgtg ctacagcgcg gcgacttgca
    11521 cctacggcag cggtgtcgaa gttctcgcct ccgcgcgcta tcgctggtga atcgccggag
    11581 gggctcgatg caggcagcgg atacgcccga tctctttcat cccgaacgca cacgctctga
    11641 acacgcgcgc gcggctcgtc ccgccgggcc gctctacgag atcgacggcg tttcgttcga
    11701 aatcgacgct cgagtgctgc tgcaacccct cacgctcacg ctgcatgcgg gccgcatgat
    11761 cgcgctcatc ggccataacg gttcgggcaa gagcacgctg ctcaagctgc tcgcgcgcca
    11821 gcagccgccc acgcgcggca ccctgcgctt tgcgggcacc gggctcggcg cctggccggc
    11881 gcgcgagttc gcgcgccgcg tggcctacct gccgcaacac ccaccggccg gcacccacat
    11941 gaccgttcgc gagctcgtcg cgctcggccg ctatccctgg cacggcgcgc tcggccgcgt
    12001 gacggacgaa gaccgcgcga tggtcgagcg cgcgctgcag atgaccgata cagcacgctt
    12061 cgccacgcag ctcgccgacc atctctcggg cggcgagcgc caacgcgtct ggctggcgat
    12121 gctggtcgcg caaggcgcgt cgtgcctgct gctcgacgaa ccgacctccg cgctcgacat
    12181 cgcccatcag atcgaggtgc tgcgcctgct gcgccgcctg accgacgaac acggactgac
    12241 ggtcgtgatc gtgctgcatg acatcaacct cgccgctcgc ttcagcgacg aattgatcgc
    12301 gctgcacacc ggacggctgc tgaccgcggg gccgcccgca gcactcatgc aggcccacgt
    12361 gcttgagtcc atctacggcg tcccgatgga catcgtccag catccgcatg gcggcggccc
    12421 gctcgcgatg gcacgctgac atgcgcaggc ggaccttgct gcgcgcggcc ggcatgatgg
    12481 cgctctgcgg cgtccgcgcg gcggcgggcg ctccgccggc gatggcgggc cgcgccgcgc
    12541 cgcgcatcgc cgtgctcgac tgggggctgg ccgagacgct gctcgccctc ggcgtggtgc
    12601 ccgcgggagt cgccgaaatc gccggctaca acgccaatgt cgtcacgcca gccgtaccgc
    12661 tggacgtgcc cgatgtcggg ctgcggcttg cgccgagtct cgaatggctc gcgcagcttg
    12721 cccctgatct cattctcgtc aattcaagcc aggagtcgca gcgcgagatg ctcgaacgca
    12781 tcgcgccagt gcgcgcattc gcggtctaca ccgacgccgg cacaccctac cggctcgcgc
    12841 agcaggccac gcgcgaactg ggcacgctat gccgctgcga gacggccgcc gaggcgctta
    12901 tcgcgcggac cgcgcaggcg ctcgacgccg gccgcgcgcg cctggccgcc gcgtcggcgc
    12961 actcgcgccc gctctatctg atccgttttt tcgacagccg ccacatcggc gtctacggag
    13021 cccacagcct gttccaggat gcgctcgacg cgctcggtct cgccaatgcg tggcacggcg
    13081 cgaccgacta ctggggcatc ggcgtcgcac cactcgaggc gctggccgcc gcgcctgatg
    13141 cgacggtgat ctacttcgaa ccgctgccgc aagacgccgg ccacacgctc gccgcaaacc
    13201 ggctgtggcg ggcgctgccg gctgtcgccg ccggccgcat cgccgccctg ccgcccttct
    13261 gggggttcgg catgctgccg tccgtgcagc gcttcggcgc ggcactggcc caggcgctcg
    13321 tcgccacgcc cggggccatg caaggaaacg caggctcatg agcggcctgc atcgcttcgc
    13381 cagtccggcc gcgcgcgcgg ctttcgcacc acccggcgcg cctggctcgc ctgtcgccgt
    13441 tttcatggtg atcgcgctcg cgttggccgt cgcgctcggc ctgtccgccg caaacctcgc
    13501 ggcggtcgcg ccgcgtgcgc tatggtggca ggcgatctgc tcgcccgacg cgcacgacgt
    13561 tcgccagatc atcgtgcacg acagttggct gccgcgcatc atggccgcat ggctcgcggg
    13621 ggcgacgctc gggctggccg gcaccctgtt ccagcaggtg ctgcgcaatc cgctcgccga
    13681 accgatgacg ctcggcgtat cggccggcgc gcaactcgcg ctgacgctcg ccgcgatctg
    13741 ggcgcccgca gtccccgctt tcgcgcgcga gcctgtcgcg ctcgccggcg cgctcggcgc
    13801 cgccgtggcc gttttcgggc tcgcctggcg cagcggcctc gcgccggtga cggtggccgt
    13861 cgccgggctc gtcgtcacgc tctggtgcgg atcgataggc gtcgcgctgc agattttcca
    13921 cgaaccctat atgcacggcc tgttcatctg gggcggcggc tcgctcgtcc agcaagactg
    13981 gcgcgacgtc gccacgctcg caccgcgcct tgccgttggc ggcacgcttg catggctgct
    14041 gcggcggccg atgacgctgc tcggcctgga cgaagccaac gcccgcagcc tcggcctcgg
    14101 cctgcgccgg ctacggctcg cgacactcgc actcgccgtg gcgctgagcg ccgccgtggt
    14161 cagcgcggtc ggcgtcatcg gcttcgtcgg gctcgccgcg ccggccatcg cccagcttgc
    14221 cggcgcgcgc cgcctgtcgc agcggctcgt gatggcgccg ctgtgcggat cgctcctcct
    14281 gtggctcacg gaccaggcgg tgcagccgct cgcccatgcg cttggcgaac tggttcccgc
    14341 cggcgccgcc agcgcgctgt tcggcgcgcc gatcatgctg tgggtgcttg cgcacatgcc
    14401 tcgcgcgcaa gccctcgaca gcagcgccgg gccggccttt cacgccgtac cgggggtctc
    14461 gatcagccgc cgcttcgtgc tcatcgccag cgcggcggtg gtgatggcag tggtctcgct
    14521 tgacttgggc cgcggcccgg acggctggca tttcagcggc cttgacgcgc tttcgcatat
    14581 cgcggtctgg cgcatgccgc gcgagttcgc cgcgctcgcg gccggcatca tgctcgcgct
    14641 ctccggcacg ctgatgcagc gcctcaccgg caatccgctc gccagcccgg aagtgctcgg
    14701 cgtctcggcc ggcgccgcgc tcggcttcat cgccctcgtg ctcggccggg ccgaggccag
    14761 tcacggggcg cagctcgcgg ccaccgcgct cggcgcattc gcgacgctgg ctgccatact
    14821 ctggttcgcg aagcgctcgg gtttcgcacc gcagcgcgtg ctcctcgtgg gcatcgccat
    14881 cggctcgctg ttccaggccg tggtcgccgt cgtgatcgcg ggcggcggcg aacgcgcagc
    14941 cattttgctc cgctggctgt ccggctcgac ctacacggtc acgcctgccg acgcgaccct
    15001 tgcgctggtg ctgtgcgcgg tgctcggcgc gctcatgccg cttacggccc gcctgctcga
    15061 tatcctgccg ctcggcgagg ccagcgcacg cgcgctcggc atgcgcacgg cactcgcacg
    15121 cgccgcgctg ttgggcctga ccgcgctgct gaccgccgcg gccacgctga ttgtcgggcc
    15181 gctttcgttc atcggcctca ccgcgccgca tttcgcccgg ctcgcaggcg ctcggcggcc
    15241 gctcgcgcag atactgcttg ccgcgccgat cggcggcatg ataatggtcg ccgccgattg
    15301 gctcggccgt acgctgctca tgccacgcga gctgcctgcc ggcctcgtcg cgacgctgct
    15361 gggcgcgccc tggctgatgt ggctgctcgc gcggcgccgc ggcggcgcct gaggaaagcg
    15421 caggccacca agcaatattc agaacatagc tgagatgaca atgattgcag aattgctgcg
    15481 gctattacgc cgattatggc ccatttccgc cctcgcgacg ctcatgggca tctcaagcgg
    15541 cgtcgccacg gcagcgttgc tggccgtcac caaccatgcg ctgcacgacg gcgccggatc
    15601 cttgccgacg ctgctcgcga gcttcgtcgg cctgtgcgtg ctcgcgcttg cgggagaagt
    15661 catctccgac atcggcaaca acttcgtcgg ccagcgcgtc atcgccacgc tgcgcaagaa
    15721 cctgtgcgaa aagatcatcg ccgcgcccat cagccagatc gagcagtttc gcagccaccg
    15781 gctgatcacg gcgctcaatc aggacatcga tgtgatcagc acgttttcct tcatgctgtc
    15841 gagcctcgcc atcgcgacgg caaccatcgc cggctgtctc ggctatctgt tcgtgatgtc
    15901 gccgctgatg ctgctgatcg ccctttgcgc catcgcgctc ggcgcgatcg tgcagtcgct
    15961 cgcccggcgc gccggcatcc gccgcttcgg cgaagcccgc gaagccgatg accacctgca
    16021 aaagcactat cgcgcaatgg tggatggcgc caaggaactt cgcatcaacc gcgaacgccg
    16081 cgcgttcgtc taccggcacg agctgtcggg cacgatcgac gcgatctttc gcatgcgcgt
    16141 gcgcgccgcc aacgtcttcg tgacggcaaa cgcgttcggc tcgctgctgt ttttcatcgt
    16201 ggtgggcctg atgctggtgc tgcagaccgg cgccgccgat gccgatcgca ccgtgatcac
    16261 gggttttgtg ctcgtattgc tctacatgaa gggtccgatt caggagatca tcggcgcgct
    16321 gccctcgatc ggccgggccc aggtggcatt caggcgcatt accgaacttt ccgcggactt
    16381 ttcctcgagc gagcctgcgc tgctgcaggc ggacgcgacg gcgagcgagc gtgccgcctc
    16441 gttcacgagc ctcgcgctgc tcgacgtcgc ttaccgcttt tccgcgccgc aggacgacag
    16501 cctcgccgcc acccagcccg atgccggcgg cttcgcgctg gggccgctgt cgctgacgat
    16561 cgaacgcggc gagacgctgt tcatcgttgg cgagaacggc tgcggcaaga ccaccctgat
    16621 caagctgctg ctcggcctct acacgcccgg cgccggtgcg ctgctggtcg acggcgcgcc
    16681 ggtgcgcgaa gacacgctcg acgattaccg ccagcgcttt tcggtggtgt tctccgacta
    16741 ttacctgttc gacacgctgc tgcccggcgc aagcgcgatc ggcgccgagg cgcaaggcta
    16801 tctggagcgg ctcgcgcttg cgcacaaggt gtcggtgcgc aacggcgcgt tcacgacgac
    16861 ggacctctcc accggtcaac gcaagcggct cgcgctcgtg cacgcctacc tcgaacgccg
    16921 gcccgtgatg gtgctcgacg aatgggccgc cgaccaggac ccgatgtttc gccgcatctt
    16981 ctacacggaa atactgcccg acctgaagcg ccagggcaaa acgctgatcg tcatttcaca
    17041 tgacgaccgc tatttccatg tcgccgatcg ggtgatcagg ctggaacaag gcaaaatcat
    17101 tcaggaggcc agcggcagga tgtttaccta tgaagatcgc ggcgcggcgc ccccccggga
    17161 ccaatgattg ccaagggcgg ccggaccgac tagacccagg aaccggggta tacctccgga
    17221 tcaggtatcg ctgaattccc gtagcaggtt gtgatagcac ccgaccaggg tgcggcgcgc
    17281 cacgtcgtcg gcgccgctgg cgttcaggcg ctggatggcg ttgtccatgt cgaacagcaa
    17341 ggcgcgctgc gcgtcggtgc ggaccaggct ctgcacccag aaaaagcagc ccagccgcac
    17401 gccgcgggtg accgggcgca cgtggtgcac gctggtggcc gggtacacca cggcatggcc
    17461 ggcgggcagc ttggcctcgt gcgtgccgta ggcatcctcg acaaccagct cgccgccgtc
    17521 gtagtcctcg ggcggggtaa ggaacagggt gatcgacacg tcggtgcgca cgcgcgcgcc
    17581 atggccgggg atgaggcgga tggcgccgtc gacgtggccg ccgaagtgct ggccgccctg
    17641 gtagcggttg aaaagcggcg gatacacctt gttgggcagc accgcgctga tgaagcgcgg
    17701 gttgcgctcc agcgaggcca gcaccgcatc gcccagttcg cgcgccacgg gggagccttc
    17761 ggctatctgc tggttgtcct tgaccggcgc gccgctatag cccgcggtgg cgcggccatc
    17821 cacccaggca tcgccggcag tctccagccg gcggcgcatc tgcgccgccg catccggagt
    17881 gagcacgtcg gggagggtga tcagcatgcc attcagaact tgtaatcgag cgtgaccagg
    17941 aaggtgcgcc ccagggccgg gaccgcccgg ccgccgtccg agggaatcag ggcgtcatag
    18001 tacgccttgt tggtcaggtt gatcacgttg aactgcacgt cgtagcgatc ggcatggtag
    18061 gcggccatgg cgtcccagcg cacatagccc ggcaccttga cgatattgtc gttggccgca
    18121 tagcgcgaag acaggtacac cggcccgccg ccgatctgcc aatgcggcgt gatggcatag
    18181 gtggtccaca gcgtcacggt gtggcgcggc gtgttgggca gggcgttgcc ctgggtgccg
    18241 tccaatgcct tcacgacgcg ggagtccagg taggtatagc cgccatacac ctgccaggcc
    18301 ttggtcagcc ggcccgccac gccgatctgg tagcccttga cccgcacgtc gccgttcagg
    18361 gtgaagtcgc cgtcggcatc ctgggtgcgg gcattgctct tttcgatctg gaacagcgac
    18421 tgcgcgaccg acaggttgcc gtcgagcaga tcccagcggc tgcccacctc gtaggagcgg
    18481 ttcgtctcgg gcggcaggtt ttcctggccg ctggtgagcg tgagcgcctc cagcgagggg
    18541 tcgaacgaag tgccgtacga cacgtagtac gactgcgcct cggtcggctg gtagatcagg
    18601 ccggtgcgca cgctggtgaa attgttggtg ttgtccgcat gcgcgggcgt gccggtgctg
    18661 ttgctgatgg aggccgaaaa acggtcccag cgcaggccgc ccaccgcctt ccagtgttcg
    18721 cccagcgaca ccgtgtcgtt gatataggcg ccgaaggagt tggcgctgga ctgggccagg
    18781 tcgtccaggg tctggaccgc attcgccggc cgcgtcgtct gcggcgggtc cagcaggggc
    18841 acgatggcca gcgtgcccag gggcagtccg ggcgccgtat cgcggtagcc ctggttgctg
    18901 tagctgtcgt gctcgatatc catgccggcc accacgtcct gcttgagcca gcccgagccg
    18961 aacttggctt ccagatcggt ggagttgtag atggagtgat tgttgatcac gcgatcgcgg
    19021 ctgaccaggc ccacgaacag ctgcgaaggc ggcagcgtcg tgaaatcgcc cgcgcccagg
    19081 gccggcgagg acgccgtggg accggtgtac agcccgcgct tgaggctggt ctcgcgggcg
    19141 tcggtcgagt aatggccgaa gcgcgtctgg ttgcgcagct tcacgttgct gttgaaggtg
    19201 tgctcgatgc gcgcggtgaa cgcctgcaca tcctggatgg tacggtcgtc ggtaaacccg
    19261 tagaaagtcg aagggctcac cggcgcgggc ctgccgttga gcgattgcac gccgtagtcg
    19321 ggctggtcgt cgttgtgctg caccagggcc gacagcgtga tctcggtcgg cgtgccgatg
    19381 cccaggcgca gcgtgggcgc gatgccgaag tcctggttct gcatcacgtc gcgcgtgctc
    19441 gacaggctct gcccgaagcc gtcgatgcgc agggccgagg tgtccgacag gggctggttg
    19501 acgtcgaagg tgccgcgttc gcggtcgtgc gtgccgatgg tggtcgatac ctcggccgag
    19561 ggcttgaggt tggcctgctt gctcacctgg ttgatggccc cgcccgtcga gccgcgcccg
    19621 aagtagagcg acgacgggcc gtacagcacg tcgatggact ccaggtcgaa ggtgtcgcgg
    19681 taatactggc cgcggtcgcg aaagccgtcc aggtagatat cgttgttggc ggtgaaaccg
    19741 cgcaggttga tgttgttgcc gatcacgccg ccctcggccg cgccgatggt cacgcccggc
    19801 acgttgcgca gcgcatcctg gaaggaactg gccgcctgcg actgcagcag cgccttgttg
    19861 accacggccg tggcctgggc gatgtcgtgc tggtccatgg tgaagcgccc gcccacgctg
    19921 gacagcggcg cctggaagtc attgccgagc gaatcggcct ccacggtcac ggtaggcagc
    19981 gccgcgacgc cgcccgtgtc ggccgcggac gggcggctca gcacgtagcc gccgccgctg
    20041 cgcgcggcct gcaagccgga gccggccagc aaggcctgca ggccggcccc ggcctcgtat
    20101 tcgccgtgca agccggcggt ggtggccgaa ccgatcacgg ccgggtcgta cgacacattg
    20161 atgcccgcct gctgcgcgta gcggtccagg gccggctgca gcgggccggc ggcaatgtcg
    20221 aagcggcgca tcgctgcctt ggcgggcgcg gcggccgtct gcgccagggc gctgcgcacc
    20281 ggcgcgatcg cgcccagcgt caggcacagc atcatcgctc cctgcagggc ggtcgccagc
    20341 ggcgccagcc ggaaagtacg ccgcggcgcg cgggcagaag aagaaacacg tggagaagca
    20401 agcatgtcgg gatctcgtct cggaaagggc gtcaggccac aaatgaccgt ctctgccctc
    20461 tatgccgaac gaaaccctcc aatcccctca atttttttcc aaaaatcttt acggcgcctg
    20521 ttccggacaa tccttctagg ctcccgccgg gaccaccgac aaccacaacg gcgtgcggcg
    20581 cacgatgcgc accggctggg tctgcgcgat gaaccgcagc gtctggtcaa tgtcgtcgag
    20641 ctggtagacg ccggtcaggc gcaggtcggc caccgaggga tcacaggcga tatgccccac
    20701 ccggtagcgc gccagttcgg ccaacagatc ggccagccgc atccggtggg cattgatcga
    20761 accgtccgcc caactggtca catcgaaatc cggctgcgcc agctgcgacg cgccatcggc
    20821 ggccaggcgc cagtattcgc cgcgccgcac gatgccggcg cgcatgcccg aggccaaata
    20881 cagttcggcc atgccgtcct
//
LOCUS       DASWPO010000068         6212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_283049, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000068 DASWPO010000000
VERSION     DASWPO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283049"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /gene="rnc"
                     /locus_tag="VGC69_04200"
     CDS             complement(<1..510)
                     /gene="rnc"
                     /locus_tag="VGC69_04200"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY0294522.1"
                     /translation="MSPHATLETRLDHRFGDQALLEQALTHRSHGARHNERLEFLGDS
                     VLNFVVAAMLFEKYGKLDEGDLSRLRANLVKQSSLVEVAQRLDLSQYLRLGEGELKSG
                     GFRRPSILADAVEAIFGAVFLDAGFDAARKVILKLYQPVMANIDPKTLGKDAKTLLQE
                     FLQGRKLPLP"
     gene            complement(539..1432)
                     /gene="lepB"
                     /locus_tag="VGC69_04205"
     CDS             complement(539..1432)
                     /gene="lepB"
                     /locus_tag="VGC69_04205"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0294523.1"
                     /translation="MSWNFALILFLLLVITGVIWGLDLAWLRKGRMARSQAAVAAYDT
                     ASFGSDMPAADRARERQEAGEKAGKAPWWVEYAVSFFPVILFVFVLRSFVVEPFRIPS
                     GSMLPTLQSGDLILVNKFSYGIRLPIIDRKVIDTGSLKRGDVIVFRYPVDPDEDYIKR
                     VVGLPGDEITYQDKNLYVNGKLVTHARDGDYFEPDRMIYVSQFNEQLGDVNHKILLDE
                     QHSQDFGPIWRFPGIEGCQYSSRGVRCVVPQGSYFAMGDNRDNSADSRYWGFVPDGNI
                     VGKAFFIWMNFSDPARIGRFH"
     gene            complement(1478..3271)
                     /gene="lepA"
                     /locus_tag="VGC69_04210"
     CDS             complement(1478..3271)
                     /gene="lepA"
                     /locus_tag="VGC69_04210"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY0294524.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRLIQRCGGLADREMSAQVLDSM
                     DIERERGITIKAQTAALHYTAQDGHVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIELGVEVLPVLNKMDLPQADPEGARQEIEDVIGIDASHAVL
                     ASAKTGVGIDEILEMVVARVPAPKGDPARPLQALIIDSWFDNYVGVVMLVRIINGVLK
                     PKDKILLMASGATHLCEQTGVFTPKSQPRPHLSAGEVGFIIAGIKDLADAKVGDTVTL
                     AGKPAAAPLPGFKEVQPQVFAGLFPVESSEYDALRDSLEKLKLNDAALQFEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPSVVYEVEQRDGTVITVDSPSRMPE
                     VGRINDIREPIVTVTLFMPQEYVGPVMTLANNKRGQQVDMNYSGRQVILKYEIPLAEI
                     VLDFFDKLKSVSRGYASMDYEFLAYRSADVVKVDLLINNDKVDALAMIVHRSNARYRA
                     REVVSKMRELIPRQMYDVAIQAAIGAEVIARENVKALRKNVLAKCYGGDISRKKKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAILQVEDK"
     gene            complement(3483..5033)
                     /locus_tag="VGC69_04215"
     CDS             complement(3483..5033)
                     /locus_tag="VGC69_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY0294525.1"
                     /translation="MYFTSVSILLARRAAVAATAATLILSGIAPAQAQQAAAPAAPAA
                     PLVALPDFTPIVEKTDPAVVNIRTTATVPVRSGPCGTDPSELFRWFFGPDFQGPGGQQ
                     QPGQGRGQGQDRQKTPPQKSTPPEERTVPRGVGSGFFISTDGYVMTNTHVISDATDIF
                     VTLTDGREFKAKVIGSDERTDVALLKIDAKDMTPLPIGDSNKIKKGQWVLAIGSPFGL
                     DSTVTAGIISAIGRDTGEYLPFIQTDVAVNPGNSGGPLINLQGEVVGINSQIISRSGG
                     FMGISLAIPIDEAMRVVDQLRATGKVTRGRIGVQIGEVTKDVADALGLPKAEGAMVSN
                     VEAKGPADAAGVQAGDVITGFNSEAIKRWSDLPRIVGDVKPGTAATLQVWRKGKSIKL
                     NVKVGEMSADKPAANPSDENSDDQGKKTPSTDNLLGLGVTDVPADQLSKLHIKGGALV
                     RDIKEGPSEQAGIQAGDIILAINDTDITNADTFAKVVAKLDKHKVVGLLVRRGDQAQW
                     VAVQPAKQ"
     gene            complement(5133..6212)
                     /locus_tag="VGC69_04220"
     CDS             complement(5133..6212)
                     /locus_tag="VGC69_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEY0294526.1"
                     /translation="MAWGLLTCGLVLAKPEAKPEAKPPEVKPDDVSRIAAKDPAQLLA
                     RIQDAASKQNYLGVFMYQQGESIQSSRMAHVVDAGGERERVETLDGQPRVYLRHNDDI
                     QYLQPERKTVRQDRRRTERFPGLVQGDPAQVLRYYNLKALDRLSRVADRQCRVVVLEP
                     RDALRYGYRLCVDIGTGLLLKAQTLDSSRRIVEQVSFSSVRLGQDVDASRVDSSWDTH
                     DWKVLEPAMKPVDMAAQGWRIAMPDGFKPVMEVARQMGPASTGNAPAGAAGTPRTVSQ
                     MVLSDGLAAISVFIEPYDAGKHHHPPAGPIRRGAINIYGRRVADYWLTALGEVPVATL
                     ERLANAVQYNAVAKAGAKPIAGTTR"
BASE COUNT         1034 a         2002 c         2079 g         1097 t
ORIGIN      
        1 gggcagcggc agtttgcggc cctgcaggaa ctcctgcagg agcgtcttgg catccttgcc
       61 cagcgtcttg ggatcgatgt tggccatcac cggctggtaa agcttgagga tgaccttgcg
      121 cgccgcgtcg aagccggcgt ccaggaatac cgcgccgaag atggcctcga ccgcgtcggc
      181 aagaatggaa ggacggcgga aaccgccgct cttcaactcg ccctcgccca gccgcaggta
      241 ctgcgacagg tccaggcgct gcgccacctc gaccagcgag gactgcttga ccaggttggc
      301 gcgcaggcgg gagagatcgc cttcgtcgag cttgccgtac ttttcgaaca gcatggcggc
      361 caccacgaag ttcagcaccg agtcacccag gaattccagc cgctcgttgt ggcgcgcgcc
      421 gtggctgcgg tgcgtcagcg cctgctcgag cagggcctgg tcgccgaagc gatgatccag
      481 gcgggtttcc agggtggcat ggggcgacat ggcgggagct gggattggcg gaaagccttt
      541 aatggaagcg gccgatgcgg gccgggtcgc tgaaattcat ccagatgaag aaagccttgc
      601 ccacgatatt accgtcagga acaaagcccc aatagcggct gtccgcacta ttgtcacggt
      661 tatcccccat ggcgaagtag ctgccctggg gcaccacgca gcgcacgccg cggctggagt
      721 actggcagcc ctcgatgccc gggaaacgcc agatcgggcc aaaatcctgg gaatgctgct
      781 catccagcag tattttatgg ttaacgtcgc ctaactgctc gttaaactgc gacacataga
      841 tcatccggtc gggctcgaag tagtcgccgt cgcgggcatg cgtcaccaat tttccgttga
      901 cgtagagatt tttgtcctgg taggtgattt cgtcgcccgg caggccgacc acgcgcttga
      961 tgtagtcctc gtccggatcg accgggtagc ggaacacgat cacgtcgccg cgcttgagcg
     1021 agccggtgtc gatgaccttg cggtcgatga tgggcaggcg gatgccgtag ctgaacttgt
     1081 tgaccaggat caggtcgccc gactgcagcg tgggcagcat cgagcccgag ggaatgcgaa
     1141 agggttcgac cacgaacgag cgcagcacga acacgaacag aatcaccgga aagaagctga
     1201 ccgcatactc gacccaccag ggcgccttgc cggccttctc gccggcctcc tggcgctcgc
     1261 gcgcccggtc ggcggccggc atgtcgctgc cgaaggacgc cgtgtcgtag gccgccacgg
     1321 cggcttgcga gcgcgccatg cggcccttgc gcagccaggc caggtccagg ccccatatca
     1381 cgccggtgat gaccagcagc aaaaacagaa tcagggcgaa gttccaactc atgagggcaa
     1441 cctataggga tacgtgacgt gcgtaatgat cgtgggatta cttgtcttcg acctgaagga
     1501 tggccaggaa ggcctcctgc gggatctcga cgctgcccac ctgcttcatg cgcttcttgc
     1561 cggccttctg cttttccagc agcttcttct tgcgggagat gtcgccgccg tagcacttgg
     1621 ccagcacgtt cttgcgcagc gccttgacgt tctcgcgggc gatcacctcg gcgccgatgg
     1681 cggcctggat ggccacgtcg tacatctggc gcgggatcag ctcgcgcatc ttggacacca
     1741 cctcgcgcgc gcggtagcgg gcattggatc ggtgcacgat catggccagg gcatcgacct
     1801 tgtcgttatt gatgagcagg tcgaccttga ccacgtcggc cgaccggtac gccaggaact
     1861 cgtagtccat cgacgcatag ccgcgcgaca cggacttgag cttgtcgaag aagtcgagca
     1921 cgatctcggc cagcgggatc tcgtacttga ggatcacctg gcggccggag tagttcatgt
     1981 cgacctgctg gccgcgcttg ttgttggcca gcgtcatgac cggccccacg tactcttgcg
     2041 gcatgaacag cgtgaccgtg acgatgggct cgcggatgtc gttgatcctg cccacctcgg
     2101 gcatgcgcga agggctgtcc acggtgatca ccgtgccgtc gcgctgctcg acctcgtaca
     2161 ccaccgacgg cgcggtggtg atgaggtcca tgtcgaattc gcgctccagg cgttcctgca
     2221 cgatttccat gtgcagcagg cccaggaagc cgcagcggaa gccgaagccc agcgcctgcg
     2281 agacttccgg ctcgaactgc agcgcggcgt cgttgagctt gagcttttcg agcgagtcgc
     2341 gcagcgcgtc gtactcggag ctttccaccg ggaacaggcc ggcgaacacc tgcggctgga
     2401 cttccttgaa gccgggcaag ggcgcggccg ccggcttgcc ggccagggtg acggtgtcgc
     2461 ccaccttggc gtcggccagg tccttgatgc cggcgatgat gaagcccacc tcgcccgccg
     2521 aaagatgcgg ccggggctgc gacttgggcg tgaacacgcc ggtctgctcg cacaggtggg
     2581 tggcgcccga agccatcagc aggatcttgt ccttgggctt gagcacgccg ttgatgatgc
     2641 gcaccagcat gaccacgccc acgtagttgt cgaaccacga gtcgatgatg agcgcctgca
     2701 gcggccgggc cgggtcgccc ttgggcgcgg gcacgcgcgc cacgaccatc tccagtatct
     2761 cgtcgatgcc cacgcccgtc ttggcgctgg ccagcacggc atggctcgcg tcgatgccga
     2821 tgacgtcctc gatctcctgc cgcgcgccct cgggatcggc ctggggcagg tccatcttgt
     2881 tgagcaccgg cagcacttcc acgcccagct cgatggcggt gtagcaattg gccaccgtct
     2941 gcgcctcgac gccctgcgag gcgtcgacca ccagcagcgc cccctcgcag gccgacagcg
     3001 agcggctgac ttcatacgag aagtcgacgt gccccggggt gtcgatgagg ttgaggttgt
     3061 agacatggcc gtcctgcgcc gtgtagtgca gcgcggccgt ctgggccttg atggtgatgc
     3121 cccgctcgcg ctcgatgtcc atcgagtcga gcacctgcgc agacatctcg cggtcggcca
     3181 acccgccaca acgctggatc aggcggtcgg ccaaggtcga tttgccgtga tcgatgtggg
     3241 cgatgatgga aaagttgcgg atatgctgca tgaagggcaa taaaaagagg aagggccgcg
     3301 tatgcgtccc tttttggatt aacccatcat tttaccgcat aggcatgaag aggcttcgag
     3361 ccgaggaccc cggaggaatc cgaagcacag cccccagtgg gggcgagcat cggaattccg
     3421 cggggcagcc tcggcggcta gagaacccgc ctctcctgct ggcgggtaaa gaacccgccc
     3481 ccttactgct tggccggctg caccgccacc cactgcgcct ggtcgccgcg gcgcaccagc
     3541 agccccacca ccttgtgctt gtccagcttg gccaccacct tggcgaaggt gtcggcattg
     3601 gtgatgtcgg tgtcgttgat ggccaggatg atgtcgcccg cctggatgcc ggcctgctcg
     3661 gacggccctt ccttgatatc ccgcaccagc gcgccgccct tgatgtgcag cttgctcaac
     3721 tggtcggccg gcacgtcggt cacgcccagg cccagcaggt tgtcggtcga gggcgtcttc
     3781 ttgccctggt cgtccgaatt ctcgtcggac ggattggccg ccggcttgtc ggcgctcatc
     3841 tcgcccacct tgacgttgag cttgatgctc ttgcccttgc gccagacctg cagcgtggcg
     3901 gccgtgcccg gcttgacgtc gcccacgatg cgcggcagat cggaccagcg cttgatggct
     3961 tcgctgttga agccggtgat gacatcgccc gcctgcacgc cggccgcgtc cgccggcccc
     4021 ttggcctcga cgttgctcac catggcgcct tcggccttgg gcagacccaa tgcgtcggcc
     4081 acgtccttgg tcacctcgcc gatctgcacg ccgatacggc cgcgcgttac cttaccggtg
     4141 gcgcgcaact ggtcgaccac ccgcatggcc tcgtcgatgg gaatggccag cgaaatgccc
     4201 atgaagccgc cgctgcgcga gatgatctgc gagttgatgc ccaccacctc gccctgcagg
     4261 ttgatgagcg ggccgcccga gttgccgggg ttgaccgcca cgtcggtctg gatgaagggc
     4321 aggtattcgc ccgtgtcgcg gccgatggcg ctgatgatgc ccgccgtcac ggtggagtcc
     4381 aggccgaagg gcgagccgat ggccagcacc cactgcccct tcttgatctt gttggagtcg
     4441 ccgatgggca ggggcgtcat gtccttggcg tcgatcttga gcagcgccac gtcggtgcgc
     4501 tcgtccgagc cgatgacctt ggccttgaac tcgcggccgt cggtcagggt gacgaagatg
     4561 tcggtggcgt cggagatgac gtgggtattg gtcatcacgt agccgtcggt ggaaatgaag
     4621 aaacccgacc ccacgccgcg cggcacggtg cgctcttcag gcggcgtgga cttctggggc
     4681 ggcgtcttct ggcgatcctg gccctgccct cggccctggc cgggctgctg ctggccgccg
     4741 gggccctgga aatccggccc gaagaaccag cggaacagct cggagggatc cgtcccgcag
     4801 ggaccgctgc gcacgggcac ggtggccgtg gtgcggatat tgaccacggc ggggtcggtc
     4861 ttttcgacga tgggggtgaa atcaggcaag gccaccagcg gggcggcagg ggcggcgggc
     4921 gcggcggcct gctgcgcctg ggccggggcg atgcccgaca agatcagggt ggccgccgtg
     4981 gcggccaccg ccgcgcgacg cgccagcagg atggacacag aagtgaaata catcagaacg
     5041 actccggaat ggcccgcgga acaacctgcg ggccgctaat cggcaaagtc tagagcagaa
     5101 aggccccaag ctgaaagtct gaaacaggac gatcagcggg tcgtgcccgc aattggcttg
     5161 gcgccggcct tggcaaccgc gttgtattgg accgcattgg ccaggcgctc gagcgtggcc
     5221 acgggcactt cgcccagggc cgtcagccaa taatcggcca cgcgcctgcc ataaatgtta
     5281 atagccccac gacggatagg gccggccggc ggatggtggt gcttgccggc gtcgtagggt
     5341 tcgatgaaaa cggagatggc ggccagccca tcggacagca ccatctggct gacggtgcgc
     5401 ggcgtgccgg ccgccccggc cggggcattg cccgtcgatg ccgggcccat ctgccgggcc
     5461 acttccatga cgggcttgaa gccgtcgggc atggcgatgc gccagccctg ggcggccatg
     5521 tcgaccggct tcatggcggg ctccagcacc ttccagtcat gcgtgtccca ggacgagtcg
     5581 acgcgcgatg cgtccacgtc ctggcccagg cgcaccgagg aaaagctcac ctgctcgacg
     5641 atgcggcggg aggaatccag cgtctgcgcc ttgagcagca ggccggtgcc gatgtcgacg
     5701 cacaggcgat agccgtagcg cagcgcgtcg cgcggctcca gcaccacgac gcggcattgc
     5761 cgatcggcca cgcggctcaa gcggtcgagc gccttgaggt tgtagtagcg cagcacctgg
     5821 gcgggatcgc cctgcaccag gccgggaaag cgctcggtgc ggcggcggtc ctggcgcacg
     5881 gtcttgcgct cgggctgcag gtactggata tcgtcgttgt ggcgcaggta gacgcgcggc
     5941 tggccgtcga gcgtctcgac gcgctcgcgc tcgccgcccg cgtcgaccac atgcgccatg
     6001 cgggaagact ggatgctctc gccctgctgg tacatgaaca cgcccaggta gttctgcttg
     6061 ctggcggcat cctggatgcg ggccagcaat tgggcgggat ccttggcggc gatccggctg
     6121 acatcgtcag gcttgacctc cggcggcttg gcctccggtt tggcttccgg cttggccagg
     6181 accaggccgc aagtcagcaa accccaggcc ag
//
LOCUS       DASWPO010000069        11985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2872337, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000069 DASWPO010000000
VERSION     DASWPO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11985
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872337"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VGC69_04225"
     CDS             complement(<1..254)
                     /locus_tag="VGC69_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0294527.1"
                     /translation="MTISFTLSTTDATALDHPIWSALNTGNASIALGNDRVRRYPVDI
                     APFVAMRDTSPESYAALRALLGPEHVGVLFTTQALTPPAGL"
     gene            complement(420..1118)
                     /locus_tag="VGC69_04230"
     CDS             complement(420..1118)
                     /locus_tag="VGC69_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0294528.1"
                     /translation="MSVWKNAVLGAGEQFDRKREVLLREAAASFNRRGYHGTSLAEIA
                     KKLGVTKAALYTYVPSKEDLLYYCHDSAMDTAIESLVKARASTGNGLQKLTMTLRHYL
                     ELMLGEEGGYVILLEENAMKPAHMRAILKRRDQFEQGLRELVSEGIADASIVLCNPKL
                     AIFNIMGALNWSRKWYRPTGNWTGPQIAVALTQMLERSLSSTPSASLFEDPSAIETDD
                     DWAEEPAAPRRRPA"
     gene            1404..2192
                     /locus_tag="VGC69_04235"
     CDS             1404..2192
                     /locus_tag="VGC69_04235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0294529.1"
                     /translation="MEEAYRPPLQGLAAVVTGGTGGIGLACARALLQAGARGVVLNGR
                     SAERAERARAGLREAFPAAEVAVALGDAASPDVARAVMRQAADTLGRIGLLVNSTGGN
                     DLPRLLHRTPLEDIPGILQRCLYAQILCSRAVLPYMQEAGGGAIINIASDAAKLPTPG
                     ESVIGAAMAGIVMFTRGLAVEGKRDGIRANVLTPSLTRGTEHYERIMADPFAGKLFGK
                     AEQMASLGVVDKEDLAGLVVFLASPAAAKITGQAISMTGGISAL"
     gene            complement(2217..3029)
                     /locus_tag="VGC69_04240"
     CDS             complement(2217..3029)
                     /locus_tag="VGC69_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0294530.1"
                     /translation="MNDNPTAARDGAPPVKVSRQQAVGIIELARPAKFNCLSSAVYAA
                     IEAAIDAFEKPGSGVRAVLVRAQGKNFCTGADLDEVLPLRDDPSGLRRFIGRGHRVLK
                     RLEACELPVVAACQGLSLAGGCELMLACDVVFAARDARFGDQHAQYGLLPGWGASQRL
                     PRLIGLRRSLDLFFTARWIDSETALQWGLVNYIEAPQQLHQAAFDYCHALASRSRTGL
                     ATMKRLARQGLEGPLQTGLQMEEADTSIGLLDPDVTEGLDAFRHRRAPRFAP"
     gene            3462..4634
                     /locus_tag="VGC69_04245"
     CDS             3462..4634
                     /locus_tag="VGC69_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294531.1"
                     /translation="MTAYAKSGLRAALAAFACGAALCAAAPALAADPIKIGFTGALSG
                     QMSLLGQGVLDGVRTYIAYINDQGGVNGRKIDLVYEDDGYDPMRSIAGGKKLSEQDNV
                     VAIVSPTGTPNVAAMVSYAQERKLPIIAPYAFSHSLTTPTKHFIFTTLPEVRLQADVL
                     GEYLLKKLNHTKIAVIYQNDDFGQDAVAGFEARMQKDATPLLAKLPFDRGTSNFSGVV
                     AQARGSGATDVVFFGIPRDAALIMREARKMGWQPQFSGHNALGDPQTFELAGASLVNG
                     AIAVAVMEPLDSKKPQVADFLARQKKYLPRSTPTTYSMHGYNAAELFVEALKRVQGEP
                     TGEKVADALEQIKDFDTGLMSPLSFSPDQHAGSQAVAFMRAEGGKWTVVTDWMKAE"
     gene            4631..5461
                     /locus_tag="VGC69_04250"
     CDS             4631..5461
                     /locus_tag="VGC69_04250"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY0294532.1"
                     /translation="MNADKRLALGEAAALVRDGDTVALGGMTLYRRPVAAALALAAAG
                     RRDLEIVTLTGGVETDLLIGAGCVRRLRSCYTGLEVAGFAPHFTRGVQDGTLELVEET
                     EYTLSYAVQAAAMRVPFLPMRGSFLQTDIPRVRPDLKVFACPVSGQDLVAVPALRVDV
                     AILHAPAADRHGNCNLQGQLALDRLLPAIAGITLVTAETIVETEALRAMPGGIQLPGL
                     FVTHVVHAPGGSLPTSCLPHWPLDMVGVLDYADAAADPASWAAWLREATQAATAEVQA
                     "
     gene            5458..6357
                     /locus_tag="VGC69_04255"
     CDS             5458..6357
                     /locus_tag="VGC69_04255"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY0294533.1"
                     /translation="MKEDANLPQRAHPGARHADGLDAAQRAQVDQMVVTMARLVQDGD
                     FLAEGIGTFLPNAAYMLAKRLHAPHSVSLCPNGNTLMLGTRTLTLGRDEFDTVPRAML
                     WLDYLQINLVYMPAIFLGGRPRWTEFMRPAQIDPTGATNNVCIGPYGRPRVRLPGSAG
                     IPDASTAARRFYYYVPRHSRQVFVPGLDFRSGAGHTADGEGGPAHITVITNLCVLRSG
                     PDGRLRVAQTHPGVRLETVREQTGFELECEPESTIAAAPGEHERRLLDTEIDPDGLRF
                     LEVLAAPARRARLRELSAREVAA"
     gene            6354..7226
                     /locus_tag="VGC69_04260"
     CDS             6354..7226
                     /locus_tag="VGC69_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0294534.1"
                     /translation="MIAELIIGGLASGSLYALIAIAVVLVLQATDVPNFAQGEMAMFS
                     TFVAYTMLTGYGAGWWVALPVAVVFAILLGIVVQQLVIRPLMGAPIMSAVIATLGLNM
                     ALHSVAAMIWGNLTNVFASPVADWPVFKLFGVNVSPDSALTIVVSVAMIAIFTLILRH
                     TRAGIALRAASQDAQVARLMGIPVNRAFALAWAMGSVAGLVAGLLVAPILFLDVNMMA
                     PLLIKGFAGAVLGGLSSLSGVFVGGLLLGVAENLMGVYISPGMGDALSFILIIVVLIV
                     APAGLFGRVKSTKV"
     gene            7252..8217
                     /locus_tag="VGC69_04265"
     CDS             7252..8217
                     /locus_tag="VGC69_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0294535.1"
                     /translation="MTPTSTSSPFAAGRARGRALAGIALLAYVAVALFGGGYFVYLLN
                     LIGIFSLVAIGLNILTGFSGQTSIGHAGFFAIGAYTSGLAALLWGMPFWLAIPLGGVL
                     ATVVGGLIALPALRLKNLYLAIATLGFGVVTQKVIFEWQSITGGGAGLHVPLPTLFGL
                     PVDDGSRMAWIILAVLAAGTWSAARLSASRTGRALKMLRESETAAACLGIRIARYKVV
                     AFALSAFYTAVAGGLYAFVVRYINPESFDFNMSVMFLAMIVIGGMGSVRGAILGAVFY
                     VAVPEAVRGVKDAPGLIFGLSLMLVVVLLPGGLTSLFQSRQKETA"
     gene            8214..8993
                     /locus_tag="VGC69_04270"
     CDS             8214..8993
                     /locus_tag="VGC69_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294536.1"
                     /translation="MTAGALTVESLSVRFGGVHALQDVGLSVGQGEIVGLIGPNGAGK
                     STLLNCISGIARPSAGTVRLGDTDLKGLRPDQIAAMGLGRVFQHPQVIAGMSVLENLM
                     VAGHRTLRYGVLAEMVGLSAVRRAEDEARRAATAVAARVGLGAYLHAVTGSLPYGHRK
                     LLELGRVILMGARLLLLDEPIAGLNDAEIDQLGRLVLALRRDAGLSILLVEHNMGLVR
                     QLCDRAVVLDAGRVIAAGTPDTVLNDPKVLLAYLGEEPEDA"
     gene            8986..9690
                     /locus_tag="VGC69_04275"
     CDS             8986..9690
                     /locus_tag="VGC69_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294537.1"
                     /translation="MLEIEGLCVSYGPIQALRDVSLRVAAGQLVGILGANGAGKSSLL
                     RTISGLVRAQGGAIRYDGRDIGAVKPESIVRMGIAHVPEGRGIFPDLTVRENLLAGAH
                     TRRDRAGIDADYAQMLSIFPALARRERQDGSMLSGGEQQMLSLARALMSRPRLLLADE
                     VSLGLAPVITKQVFAHLQTLREQGITVLVVEQNARLLLRMADYVYVLKHGRLAMQGSA
                     AEMAAHPALTRAYLGE"
     gene            9721..11343
                     /locus_tag="VGC69_04280"
     CDS             9721..11343
                     /locus_tag="VGC69_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0294538.1"
                     /translation="MHDYPLQERTIGRVLAEKARRIPDKTFLIWQGRRYSYGELETMT
                     NRYANGFLARGIRHGDHVAVLLPNCPEFFWVVWGLGKIGAVAVPLNTAAKGDMLRYFV
                     DNADAICMVSGSEYLERVVAVAGDLPKVREYFYHGSACPLRECQAAMPGVAVRALSEL
                     VSDDASAPPAGRVAHDDVSLIMFTSGTTGPSKGVMCPHSQGHAVGRSLARDFGYRGDD
                     VLYTCLALFHGNGIYDSCFAALWADAAIALSPRFSATRFWDDIRATGATQFNTLGAMT
                     NIIWKLPPGPHERQTALRLCMAVPVPKEIYAEFQARYGVTLTSVFAMTENFAMTRFTP
                     EDPPEKAGSAGRPGDDCKLRIIDDHEQDLPAGQVGEILMYPVEPGSMMKGYYKMPEKT
                     AAEFLDGWFRTGDRGYLDEDGYLYFVDRKKEAIRRRGENISAYEVELILCRHPAILEA
                     AAIPVPSELSEDDVMVYVVLKDGEALTHEEVIHFCAEHMSYFMVPRFVEFIERLPKTA
                     SEKLEKYKLKQDAEARRSELWDREREGIAVRR"
     gene            complement(11416..>11985)
                     /locus_tag="VGC69_04285"
     CDS             complement(11416..>11985)
                     /locus_tag="VGC69_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0294539.1"
                     /translation="PGDVARLDLALSRAEARAGLPPQTLAAVLVATETAAAVLALAEF
                     RQPLPRLAAMLWGGEDLAGDLGVPRNRDRQGHYRAPFQLARNLMLMAAAATGALPIDA
                     VYVDVRDGDGLGAESEAARADGFLAKAAIHPEQVPVINQAFSASEEERAWAARVVQAL
                     DGGATGLAVIDGKMVDAPHLRLARRILSR"
BASE COUNT         1802 a         3935 c         4252 g         1996 t
ORIGIN      
        1 aggccggcag gcggggtcag ggcttgggtc gtgaacaata cgccgacgtg ctcgggcccc
       61 agcagcgcgc gcagcgcggc gtaggattcg ggggaagtgt cccgcatggc gacgaaaggc
      121 gcgatgtcga ccggatagcg ccggacccgg tcgttgccca gggcaatgga ggcgttgccg
      181 gtattcagcg cggaccagat ggggtggtcg agtgcggttg cgtctgtggt ggaaagcgtg
      241 aaggaaatcg tcatggggaa tcgaggttgg gctgcgattt gcaccttgtt gcggccgagg
      301 cgcctggcag cagtatgacg aattcttcgc cgccgcgccg ggcgaacggg tcccgggcga
      361 gggcgcgcgg tcgcccatgc cgggcgcggt cgaccccgat gcggggcaaa gcgggcagcc
      421 taggccggcc ggcggcgcgg cgcggcgggt tcctcggccc agtcgtcgtc ggtttcgatg
      481 gccgaggggt cttcgaacag ggaggcgctg ggcgtgctgg acagcgagcg ttccagcatc
      541 tgcgtcaggg cgacggcgat ttgcggccct gtccagtttc cggtcgggcg gtaccacttc
      601 cggctccagt tgagcgcgcc catgatgttg aagatggcca gcttgggatt gcagagcacg
      661 atgcttgcgt cggcaatgcc ttcggagacc agttcgcgca gcccctgctc gaattggtcg
      721 cggcgcttga ggatggcccg catgtgggcg ggtttcatgg cgttttcctc gagcaggatc
      781 acgtagccgc cttcttcgcc gagcatcagt tcgagatagt ggcgcagcgt catggtgagc
      841 ttctgcaggc cattgccggt gctggcgcgc gccttgacca ggctttcgat ggcggtgtcc
      901 atcgccgagt cgtggcagta gtagagcaag tcttctttgc tgggcacgta cgtgtacagc
      961 gccgccttgg tcacccccag cttcttggcg atctcggcca gcgaggtgcc gtggtagccg
     1021 cggcggttga acgatgccgc ggcctcgcgc agcaggactt cgcgcttgcg gtcgaattgc
     1081 tcgccggcgc ccaggacggc atttttccag acactcatgc gcgaatctct ccatgtaggt
     1141 ggggccgcgc catcggggag cgtggcgcgc gggaaagcgg gtgcgggatc ggcggtgcgt
     1201 cagtctgcat ggcggtgtgt ggtcgaaggt tgccgtcggc gggctgccgt gggcttgggc
     1261 aagcgcggca aggcccggaa cggaaagcgg tccattttaa gcgcatccgg tttagcgatc
     1321 tatccagacg gttagtctat tgtctagtaa gttagtttga gtttagactg ccagccagga
     1381 gaccgcgagg aaggaagcga cccatggaag aggcatatcg tcccccgttg caagggctgg
     1441 ccgccgtcgt cacgggcggc accggcggca tcggcctggc ctgcgcgcgg gcgctgttgc
     1501 aggccggcgc ccgcggcgtg gtgctcaacg gccgcagcgc cgagcgcgcc gagcgggccc
     1561 gggccgggct gcgcgaggca tttcccgccg ccgaggtggc cgtggcgctg ggcgacgcgg
     1621 ccagccccga cgtggcccgg gccgtgatgc gccaggccgc cgatacgctc ggccgcatcg
     1681 gcctgctggt caattccacg ggcggcaacg acctgccccg gctgctgcac cgcacgccgc
     1741 tggaagacat ccccggcatc ctgcagcgct gcctgtacgc gcagatcctg tgcagccgcg
     1801 cggtgctgcc ttacatgcag gaagcgggcg gcggggccat catcaacatc gcttccgacg
     1861 ccgccaagct gcccaccccc ggcgaaagcg tgatcggcgc ggccatggcc ggcatcgtca
     1921 tgttcacgcg cggcctggcg gtcgagggca agcgcgacgg catccgcgcc aatgtgctca
     1981 cgccttcgct gacgcgcggc accgagcact acgagcgcat catggccgat ccctttgccg
     2041 gcaagctgtt tggcaaggcc gagcagatgg cctcgctggg cgtggtcgac aaggaggatc
     2101 tggccggcct ggtggtgttc ctggcctcgc cggcggccgc caagatcacc gggcaggcca
     2161 tcagcatgac ggggggtatt tccgcgttgt gagcgcatgg cggctgcccg ggcggctcag
     2221 ggggcgaagc gaggcgcgcg ccgatggcgg aaggcgtcca gcccttccgt gacgtcgggg
     2281 tcgagcaggc cgatcgaggt gtcggcttct tccatctgca ggcccgtctg cagcgggcct
     2341 tccaggccct ggcgcgccag ccgcttcatg gtggccagcc cggtgcggct gcgcgaggcc
     2401 agcgcgtggc aatagtcgaa cgcggcctgg tgcagctgct gcggcgcttc gatgtagttc
     2461 accagccccc attgcagggc cgtttccgaa tcgatccagc gcgcggtgaa aaacaggtcc
     2521 aggctgcgcc gcaggccgat cagccgcggc aggcgctggc tggcgcccca gccgggcagc
     2581 aagccgtatt gcgcgtgctg gtcgccgaag cgcgcgtccc gggcggcgaa gaccacatcg
     2641 caggccagca tcaactcgca gccgcccgcc agcgacaggc cctggcaggc cgccacgacc
     2701 ggcagttcgc aggcctccag gcgcttgagg acgcgatggc cgcggccgat gaatcgccgc
     2761 agcccggacg ggtcgtctcg caggggcagc acctcgtcga ggtcggcgcc ggtgcagaaa
     2821 ttcttgcctt gggcccggac caggacggcg cgcacgccgg agccgggttt ctcgaatgcg
     2881 tcgatggccg cttcgatggc ggcgtagacc gccgacgaaa ggcaattgaa tttcgcgggg
     2941 cgggccagct cgatgatgcc gactgcctgc tggcgcgata ccttgacggg cggcgcgccg
     3001 tcccgtgccg ctgttgggtt gtcgttcatg ggtctctctc ctccggggcg atcttcctgg
     3061 gccattgaaa gcctctttcc aggcaacgat atgcgccttg aaaccacgtg tcaatttgtc
     3121 taaccaattg gtcagatttt tgcttgtatg tggcgccgta tcttttgtga tgtttttatt
     3181 taaataattg ttttatatga aatttataaa gaaataggtg gcgtattgat ggtctttaaa
     3241 gacacctttt tatgatggtc agaatatcga ccatccggtc agttagttta ttgacagccg
     3301 gttccgcact gggataatct gtgtccagac cgggcgggcc ggacgcgatc gacgcgttcg
     3361 ggacggctgt cgaaaagggc catgccggcg cagaccggct tgcccggcgg tgcggctgca
     3421 gcatccgtcg cacataaata aaacggaagg aggagacaat catgactgca tacgcgaagt
     3481 ccgggctgcg cgcggccctt gctgctttcg cttgcggggc cgccttgtgc gcggccgcgc
     3541 cggccctggc cgccgatccc atcaagatcg gtttcaccgg cgcgctttcc gggcagatgt
     3601 cgctgctggg gcagggggtg ctcgatggcg tgcgcaccta catcgcctac atcaacgacc
     3661 agggcggcgt gaacggccgc aagatcgacc tggtctacga agacgacggc tacgatccga
     3721 tgcgctcgat cgccggcggc aagaagctgt ccgagcagga caatgtggtg gccatcgtct
     3781 cgcccaccgg cacgcccaac gtggcggcca tggtctccta cgcgcaggaa cgcaagctgc
     3841 ccatcatcgc gccctacgcg ttcagccatt ccctgaccac gccgaccaag catttcatct
     3901 tcaccacgct gcccgaggtc cggctgcagg ccgacgtgct cggcgaatac ctgctcaaga
     3961 agctcaatca caccaagatc gcggtcatct accaaaacga cgacttcggc caggatgccg
     4021 tggcgggctt cgaggcgcgc atgcagaaag acgcgacgcc gctgctggcc aagctgccgt
     4081 tcgaccgcgg cacgtcgaac ttcagcggcg tggtggccca ggcgcgggga tccggcgcga
     4141 ccgacgtggt gttcttcggc atcccgcgcg atgcggcgct gatcatgcgc gaggctcgca
     4201 aaatgggctg gcagccgcag ttcagcgggc acaacgcctt gggcgacccg cagaccttcg
     4261 agctggccgg cgcttcgctg gtcaatggcg cgatcgccgt ggcggtcatg gaaccgctcg
     4321 attcgaagaa gccgcaagtg gccgatttcc tcgcgcgcca gaagaaatac ctgccgcgca
     4381 gcacgcccac cacctacagc atgcacggct acaacgccgc cgagctgttc gtcgaggcgc
     4441 tcaagcgggt gcagggcgag cccaccggcg agaaggtggc cgacgccctg gagcagatca
     4501 aggacttcga tacgggcctg atgagcccgc tcagtttctc gccggaccag cacgccggca
     4561 gccaggcggt ggcgttcatg cgcgccgagg ggggcaaatg gaccgtggtc accgactgga
     4621 tgaaggcgga atgaacgctg acaagcgcct cgccctgggc gaggcggcgg cgctcgtgcg
     4681 cgacggcgac accgtggcgc tgggcggcat gaccctgtac cggcgcccgg tcgcggccgc
     4741 gctggcgctg gctgccgccg gccgtcgcga tctggagatc gtcacgctca ccggcggcgt
     4801 cgagaccgac ttgctgatcg gggccggctg cgtgcgccgg ctgcgcagct gctataccgg
     4861 gctggaggtg gcgggcttcg cgccgcattt cacgcgcggc gtgcaggacg gcacgctgga
     4921 actggtcgag gagaccgaat acaccctcag ctatgccgtg caggcggcgg ccatgcgcgt
     4981 ccccttcctg ccgatgcgcg gcagcttttt gcagaccgac attccccgcg tgcgtcccga
     5041 cctgaaagtc ttcgcctgcc cggtgagcgg acaggatctg gtcgcggtgc ccgcgctgcg
     5101 ggtcgacgtc gccatcctgc atgcgcccgc cgccgaccgc catggcaact gcaatctgca
     5161 agggcagttg gcgctcgacc gcctgctgcc cgcgatcgcc ggcattaccc tcgtcacggc
     5221 tgaaaccatc gtcgaaaccg aggcgctgcg ggccatgccg ggcggcatcc agctgccggg
     5281 cctgttcgtg acccacgtgg tccatgcgcc gggcgggagc ctgcccacgt cgtgtctgcc
     5341 tcactggccg ctggacatgg ttggcgtgct ggattacgcc gacgcggccg ccgatccggc
     5401 cagctgggcc gcctggctgc gcgaggcgac ccaggccgcg acggcggagg tccaggcatg
     5461 aaagaagacg cgaacctgcc gcagcgcgcc cacccgggcg cgcggcatgc cgatggcctg
     5521 gacgcggcgc agcgcgcgca ggtcgaccag atggtcgtga ccatggcgag actggtacag
     5581 gacggcgact tcctggccga gggcatcggc accttcctgc ccaacgccgc ctacatgctg
     5641 gccaagcgcc tgcacgcgcc gcacagcgtc agcctgtgcc ccaacggcaa tacgctgatg
     5701 ctgggcacgc gcaccctgac gctgggccgc gacgagttcg acaccgtgcc gcgcgccatg
     5761 ctgtggctgg actatctgca gatcaatctg gtctacatgc cggccatatt tctcggcggg
     5821 cggccgcgct ggaccgaatt catgcggccg gcccagatcg acccgaccgg cgccaccaac
     5881 aacgtctgca tcgggccgta cggccggcct cgcgtgcggc tgcccggttc cgcgggcatc
     5941 cccgatgcct ccaccgccgc gcggcgcttt tattactacg tgccgcgcca ttcccgccag
     6001 gtgttcgtgc ccgggctcga tttccgcagc ggcgccggcc acacggcgga cggcgagggc
     6061 gggccggcgc acattaccgt gatcaccaat ctgtgcgtgc tgcgcagcgg tcccgacggc
     6121 cggctgcggg tggcgcagac gcaccccggc gtgcgcctgg aaaccgtgcg cgagcagacg
     6181 ggcttcgaac tcgagtgcga accggaatcg accatcgcgg cggcgcccgg cgagcatgag
     6241 cggcggttgc tcgacacgga gatcgacccc gacggcctgc gctttctgga ggtgctggcc
     6301 gcgccggccc ggcgtgcccg gctgcgcgaa ctgagcgcgc gggaggtggc ggcatgatcg
     6361 ccgaactcat tattggcggc ctggcctcgg gcagcctgta cgcgctgatc gccattgccg
     6421 tggtgctggt gctgcaggcc accgacgtgc ccaatttcgc gcagggcgaa atggcgatgt
     6481 tctcgacctt cgtcgcctac accatgctca ccggctacgg cgcgggctgg tgggtggcgc
     6541 tgccggtcgc ggtggtcttc gcgatcctgc tgggcatcgt ggtgcagcaa ctggtcattc
     6601 ggccgctgat gggcgcgccc atcatgagcg cggtgatcgc cacgctgggc ctgaacatgg
     6661 cgctgcacag cgtggcggcc atgatctggg gcaacctgac caatgtgttc gccagtccgg
     6721 tggccgactg gccggtgttc aagctattcg gcgtgaacgt gtcgccggac agcgcgctga
     6781 ccatcgtggt gtcggtggcg atgatcgcca tcttcacctt gatcctgagg catacgcgcg
     6841 ccggcatcgc cttgcgcgcc gccagccagg atgcccaggt cgcccggttg atgggcatcc
     6901 cggtcaaccg ggccttcgcg ctcgcctggg ccatgggcag cgtggccggc cttgtcgccg
     6961 ggctgctggt cgcgccgatt ctcttcctgg acgtcaacat gatggcgccg ctgctcatca
     7021 agggtttcgc cggcgcggtc ctgggcggcc tgtcgagcct gtcgggcgtg ttcgtcggcg
     7081 ggctgctgct gggcgtggcc gagaacctca tgggggtgta catctcgccg ggcatgggcg
     7141 acgcgctgtc gttcattctc atcatcgtcg tactgatcgt cgcgccggcc ggcctgttcg
     7201 gccgcgtgaa atccaccaaa gtataggagc cgcccggtgc cggactcgat catgaccccg
     7261 acctcgacgt cttccccgtt cgccgccggg cgcgcgcgcg gccgcgcgct ggccggcatc
     7321 gcactgctgg cctacgtcgc ggtggcgctt ttcggcggcg gctatttcgt ctacctgctc
     7381 aacctgatcg gcatcttctc cctggtggcc atcgggctga acatcctgac gggtttttcc
     7441 ggccagacca gcatcggcca cgcgggcttc ttcgccatcg gggcctatac ctccggcctg
     7501 gccgccctgc tgtggggcat gccgttctgg ctcgccattc cgctgggcgg cgtgctggcc
     7561 acggtggtgg gcggactgat cgcgctgccg gcgctgcggc tgaagaacct ctacctggcc
     7621 atcgccacgc tgggtttcgg cgtggtgacc cagaaagtga ttttcgaatg gcaatcgatc
     7681 accgggggcg gagcggggct gcacgtgccg ctgccgacgc tgttcgggct gcccgtggac
     7741 gacggcagcc gcatggcctg gatcatattg gcggtgctgg ccgcgggcac ctggagcgcg
     7801 gcgcggctat cggccagccg caccggccgc gccctgaaaa tgctgcgcga gagcgagacg
     7861 gcggccgcct gcctgggcat acgcatcgcg cgctacaagg tggtggcctt tgcgctgagc
     7921 gccttctaca cggcggtggc gggcggtctc tacgccttcg tcgtgcggta catcaacccg
     7981 gagagcttcg acttcaacat gtcggtgatg ttcctggcca tgatcgtcat cggcggcatg
     8041 ggcagcgtgc gcggcgccat cctgggcgcg gtgttctatg tggcggtgcc cgaggccgtg
     8101 cggggcgtca aggacgcgcc cggcctgata ttcggcctgt cgctgatgct ggtggtggtg
     8161 ctgctgccag gcggcctgac cagcctgttc cagtcccggc agaaggaaac ggcatgacgg
     8221 ccggcgcgct gacggtggag tcgctgagcg tgcgcttcgg cggcgtgcac gccctgcagg
     8281 acgtgggcct gtccgtgggg cagggcgaga tcgtggggct gatcggcccc aacggcgcgg
     8341 gcaagagcac cttgctcaac tgcatctcgg gcattgcccg ccccagtgcc ggcacggtgc
     8401 ggctgggaga caccgacttg aagggactgc ggccggacca gatcgccgcg atggggctgg
     8461 ggcgggtgtt ccagcacccg caggtgatcg ccgggatgag cgtgctggaa aacctgatgg
     8521 tggccggcca ccgcaccctg cgctacggcg tgcttgccga aatggtcggc ttgtccgcgg
     8581 tgcgccgcgc cgaggacgag gcgcgccgcg ccgccaccgc cgtggccgcg cgcgtcggcc
     8641 tgggcgcgta tctgcatgcc gtcacgggca gcctgcccta tgggcatcgc aagctgctgg
     8701 aactggggcg ggtcatcctg atgggcgcgc gcctgctgct gctcgacgag cccatcgcgg
     8761 gcttgaacga cgccgagatc gatcaattgg gccggctggt cctggcgctg cgccgcgacg
     8821 cggggctgtc catcctgctg gtggagcaca acatggggct ggtgcgccag ctgtgcgacc
     8881 gcgcggtcgt gctggacgcg ggccgggtga tcgccgccgg cacgcccgac acggtgctca
     8941 acgaccccaa ggtgctgctg gcctacctcg gcgaggagcc cgaagatgct tgagatcgag
     9001 ggcttgtgcg tgagctacgg gcccatccag gcactgcggg acgtgtcgct gcgcgtggcg
     9061 gccggccagc tggtgggcat cctgggggcc aatggcgcgg gcaagagcag cctgctgcgc
     9121 accatcagcg gcctggtccg cgcgcagggc ggcgccatcc gctatgacgg cagggacatc
     9181 ggcgccgtca agcccgaaag catcgtgcgg atgggtatcg cccacgtgcc cgaggggcgc
     9241 gggatctttc ccgacctgac ggtgcgcgag aacctgctgg cgggcgcgca cacgcgccgc
     9301 gaccgcgcgg gcatcgatgc cgactatgcg cagatgctgt cgatctttcc ggcgctggcg
     9361 cgccgcgagc ggcaggacgg ctccatgctg agcggcggcg agcaacagat gctgtcgctg
     9421 gcgcgcgcgc tgatgtcgcg ccccaggctg ctgctggccg acgaggtcag cctggggctg
     9481 gcgccggtca tcaccaagca ggtgttcgcc cacctgcaga ccttgcgcga gcagggcatc
     9541 acggtactcg tggtcgaaca gaatgcgcgg ctgctgttgc ggatggccga ctacgtctat
     9601 gtgctcaagc acggccggct ggccatgcag ggcagcgcgg cggagatggc cgcgcatccg
     9661 gcgctcaccc gggcctatct gggggagtga cctcatgaaa aacaggcgag gagacaagcg
     9721 atgcacgact atcccctgca ggagcggacc ataggccgcg tcctggcgga aaaggcccgg
     9781 cgcataccgg acaagacttt tctgatctgg cagggccggc gctatagcta cggcgagctg
     9841 gaaaccatga ccaaccgcta tgccaacggc tttctggccc gcggcatccg ccacggcgac
     9901 cacgtggcgg tgctgctgcc caactgcccg gagttcttct gggtggtgtg ggggctgggc
     9961 aagatcggcg cggtggccgt gcccctgaac acggccgcca agggcgacat gctgcgctat
    10021 ttcgtcgaca acgccgatgc gatctgcatg gtgtccggca gcgaatatct cgagcgcgtc
    10081 gtcgccgtgg ccggggacct gcccaaggtg cgcgaatact tctaccatgg ctcggcctgc
    10141 ccgctgcggg aatgccaggc ggcgatgccg ggcgtcgcgg tgcgcgccct gtccgaactg
    10201 gtctcggacg atgcgtcggc gccgccggcc gggcgcgtcg cccacgacga cgtcagcctc
    10261 atcatgttca cctccggaac caccgggcct tccaaggggg tgatgtgccc gcattcgcaa
    10321 ggccacgcgg tgggccgttc gctggcgcgc gatttcggct atcgcggcga cgatgtgctt
    10381 tatacgtgcc tggcgctctt tcacggcaac ggcatctacg attcctgctt cgccgcgctg
    10441 tgggccgacg cggccattgc cttgtcgccg cgcttttcgg ccacccgctt ctgggacgac
    10501 atccgcgcga cgggcgccac ccagttcaac acgctgggcg ccatgaccaa catcatctgg
    10561 aagcttccgc ccggcccgca cgagcgccag acggccctgc ggctgtgcat ggcggtgccg
    10621 gtgcccaagg agatctacgc ggaattccag gctcgctacg gggtgacgct gacctcggtg
    10681 ttcgccatga ccgagaactt cgcgatgacg cgcttcacgc ccgaagaccc gcccgagaag
    10741 gcgggttcgg ccggcaggcc gggcgacgac tgcaagctgc gcatcatcga cgaccatgag
    10801 caggacctgc cggccgggca ggtgggcgag atactgatgt atccggtgga gcccggctcc
    10861 atgatgaagg gctattacaa gatgcccgaa aagacggccg ccgaattcct cgacggctgg
    10921 ttccgcaccg gcgaccgggg ctatctggac gaggacggct acctgtactt cgtcgaccgc
    10981 aagaaagagg ccatccggcg ccggggcgag aacatctcgg cctacgaggt cgagctcatc
    11041 ctctgccgcc atcccgccat cctggaggcg gcggccattc ccgtgccgtc ggagctgagc
    11101 gaggacgacg tcatggtcta cgtcgtgctc aaggacggcg aggcgttgac gcacgaagag
    11161 gtgattcatt tctgcgccga gcacatgagc tatttcatgg tgccgcgctt cgtcgagttc
    11221 atcgagcgcc tgcccaagac cgccagcgag aagctcgaga agtacaagct caagcaggat
    11281 gccgaggccc gccgcagcga gctgtgggac cgcgagcgcg aagggatagc ggtgcgccgc
    11341 tagggagatg ggcggccgtg gggcttgccg cgccgcgcag gcgccgcggc aagccccacg
    11401 gccggcggaa cgggctcagc gggacaggat gcgccgcgcc agccgcagat ggggcgcgtc
    11461 caccatcttg ccgtcgatga cggccaggcc ggtggcgccg ccgtccagcg cctggaccac
    11521 gcgtgccgcc caggcccgct cttcttcgct ggccgagaag gcctggttga tgacgggcac
    11581 ttgctccgga tggatcgcgg ccttggccag gaagccgtcc gcgcgcgccg cttcgctttc
    11641 cgcccctagt ccatcgccgt cgcgcacgtc gacgtacacg gcgtcgatcg gcaaggcgcc
    11701 ggtggcggcc gcggccatca gcatgaggtt gcgcgccaat tggaagggcg cgcggtaatg
    11761 gccttggcgg tcgcggttgc gagggacgcc gagatcgccc gccaggtctt ctccgcccca
    11821 cagcatggcg gccaggcgcg gcagcggctg gcggaattcc gccagggcga ggacggcggc
    11881 cgcggtctcg gtggcgacca ggacggcggc cagggtttgc ggaggcaggc cggcgcgcgc
    11941 ttcggcgcgg ctcagcgcca aatccagccg ggcgacgtcg cccgg
//
LOCUS       DASWPO010000070         8582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1712111, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000070 DASWPO010000000
VERSION     DASWPO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712111"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..880
                     /locus_tag="VGC69_04290"
     CDS             83..880
                     /locus_tag="VGC69_04290"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294540.1"
                     /translation="MLEVRNIKKYYATPSGVARAVEDVSFSVARGEFLAIVGGSGAGK
                     TTLLRMLAGLLEPTSGEIRFNGRAVAGPPKAFAVVFQDYARSLYPWFSVHRNVELPLV
                     NLIPDPRKRRARVDDMLERVGLHGHARKRPHQLSGGQQQRVAIARALAYAPDVLIMDE
                     PFASVDAQTRNELEDLMLTLKQDSGMTVVFVTHDVDEAVYMSDRVLVLGRSPSTVATE
                     VGIDLPASRDQISTKALPAFAAARTAILVSIREQAASGRASSLPRAA"
     gene            935..1903
                     /locus_tag="VGC69_04295"
     CDS             935..1903
                     /locus_tag="VGC69_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294541.1"
                     /translation="MRLLRLSSFSAWAAALATAAALGSAPAFAADAPIPVNIAVIPTL
                     DAGPLLLAKDKGIFARHGLDVTINAGGTGATIIPTVLSQQYGIGYSNVISDLEAIDKG
                     LPLLLVHATYGQPADQARDPYRVYVSPNGPIKQARQLAQANIGTSSIQNIAEWTTRKA
                     LENLGVTDFSKLRWTKVVGDEAYSTVKAGTLDAVWLPQPTGAAAVAAGLVPILSANSL
                     SLPGAVGGYYITSKAYAQSHPDVLARFNASLDEANLYAAAHPQEGKDIVVKVLQFDQK
                     LVEAADLNDYPNDPGVDKLKIIASDLVRYGFIKKAPDPDAIFWKVR"
     gene            1982..2422
                     /locus_tag="VGC69_04300"
     CDS             1982..2422
                     /locus_tag="VGC69_04300"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0294542.1"
                     /translation="MAETKTETQPETKQVQAQTIGLSGRYLIDAEGNIFVSDSASGRE
                     EGTVAPEPTHFLLSALAVCALGSVEKEARERGVTVAGGTAQVTSVRDEHDRTRFANIQ
                     IDVTVRGVTQAQAELLVAHFTGNCPIYNTLRRGGPIAVSVRAEA"
     gene            2455..3636
                     /locus_tag="VGC69_04305"
     CDS             2455..3636
                     /locus_tag="VGC69_04305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0294543.1"
                     /translation="MPKRALHELAKPAEPASVPGSPLARVLAEQPFLLGIFLPLQNGA
                     WSQSTYPRGTGWTFEYNRSLVQRADELGFELGFGLSGWLPKGGHGGRQRYRENHLDPF
                     IAAVGLAAVTRRMILAGTIHILYRWHPLMLAKQLVTADHVAGGRFGVNIVTGSSVDGA
                     RMFGIQRAEHDQRYRQADEFVRLMKDLWQGDEALSYDGSWPMDEAYISPRPRYGRPIL
                     LSASSSAAGFDYGAEHSDVVFTSSPVGEQFEASIDVLPAHVADIKRRARERGRSVKVI
                     INATVITGQTDATAQAYYDAILDHADDESLASFDREHRSSDSQAWVKHDARARAVGGH
                     IHVVGSWDTVADKLERLHAAGMDGVQLTFYDYLPEMELFATHVIPRLEAKGLRHPYVH
                     R"
     gene            3673..4974
                     /locus_tag="VGC69_04310"
     CDS             3673..4974
                     /locus_tag="VGC69_04310"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180772.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HEY0294544.1"
                     /translation="MPHTPFHLAWFISKGYGPKAWRQPWGGPNPHQWVHPDLFVDLAR
                     SLERAAFDYVMIEDSSNIPYTYGNSHDVYLRYAVDSPKLDPAVLATYLIQATQRLGVV
                     TTLSTSEYNPFQLARLTNTLDHVSRGRAGWNLVTGSNDGGAQNFGLDRQYPHDQRYDM
                     ADEYAELVVKLWESWDADAMVIDREAGIFADPAKVHPVHFEGKFFRSRGPLAGPRSPQ
                     GRPVICQAGGSPRGRVFASRWAETIITSARSVAAMKAFRDDVRKRAAEQGRNPDDIKC
                     LFLINPIVDQSREGAEARRQAQLADAASHLEMHLASLSRTSGIDFSVYDLDEPLPELK
                     SNGHQTLVAQYTGRTLREIAATGKGLSDVDLVGTPRDVAAKLAEIIDAVGGDGFLFME
                     PELTRRYVAEIADGLVPELQRLGAVRKHYEHERFRDNLLAF"
     gene            complement(4984..6351)
                     /locus_tag="VGC69_04315"
     CDS             complement(4984..6351)
                     /locus_tag="VGC69_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0294545.1"
                     /translation="MDISSDMLASIWASLGGNARDPEQVGFTTAGALPSAFPVTGLAA
                     ASMAAAALAIETLRQAAGRQAAPIVVDRRLASIWFSTSLRPIDWQMPPVWDPVAGDYP
                     TRDGWIRLHTNAPHHRAAAERVLGRQTDRAGMAAAVAAWDKRELETAVVEAGGCAAQM
                     RSAEEWSRHPQGQAVAAEPLVHMETHAAPSGHRDVDPARPLAGLKVLDLTRVLAGPTA
                     TRFLAAYGAEVLRIDPPHWDEPGTVPEVTLGKRCARLDLRTPQGRAVFEQLLAGADIM
                     LHGYRSDALERLGFGAAWRRRLNPGLIDVSLDAYGWSGPWATRRGFDSLMQMSTGIAH
                     AGMQWKQAGKPCPLPVQALDHATGYLLAAAAVRGVAARLGGGALRARLSLARTAKLLL
                     DQGTGHPQEPAFAPETAADQAPEIERTVWGDARRIAWPITLQGCRAGWAHPASPLGSA
                     AAQWR"
     gene            6445..7635
                     /locus_tag="VGC69_04320"
     CDS             6445..7635
                     /locus_tag="VGC69_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0294546.1"
                     /translation="MKTINLERSMSWIPARAALALLDVRPQTLYANVSRGRIRAKPDP
                     RDPRRSLYHRSDVERLAGRRSGRRSAASVAQQAMEWGDPVLASGISTVEHGRLWYRGR
                     DAVELADTQTLEHVAGLLWNAEGIAFGAGRQRARSRARTVSPLAAALSTLAMRVDLDP
                     PSRGRARQALIPEAVRLLDDLIGAMLGRALESGMPVHRRMAAYWRAPRAQDCLRRALV
                     LLADHELNASTFATRVAVSTGTSLSASLMAGLATLAGPLHGGASLGILALRAAAQRDG
                     AAASVREYLARGDLLPAFGHPLYPAGDPRAAALLAHFKLTKVYAALRDEAMALTGEQP
                     SIDFALAAVSDALGLPAEAPFVIFALARSVGWVAHALEQIETGHLIRPRAHYVGVPIT
                     SAAA"
     gene            7833..8069
                     /locus_tag="VGC69_04325"
     CDS             7833..8069
                     /locus_tag="VGC69_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0294547.1"
                     /translation="MQVAKWGNSLAVRLPAALVEVLQLREGDEIEIVVDDPRTFSVRK
                     KAAVDELMERLRQFRGIMPPDFKFDREEANSRGR"
     gene            8059..8463
                     /locus_tag="VGC69_04330"
     CDS             8059..8463
                     /locus_tag="VGC69_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0294548.1"
                     /translation="MADKVFLDSNVILYSLSSDQDKIRAAVSLLARRPTISVQVLNEV
                     ANVCTTKLRMAWDKTDLFLQEMRDLCRIMPLTIEAHENAVQLAQRYRLPFYDACIAAS
                     ARAANCRVLYTEDLHHGLVVEDSLLIQNPFKR"
BASE COUNT         1371 a         2941 c         2856 g         1414 t
ORIGIN      
        1 tctcggccgg ctttggcgtg ctggaccgcc ttctcgggaa atggagcggc gggcgctgag
       61 caggcggccg cgcgaggaaa tcatgctgga agtccgcaat atcaagaagt actacgcaac
      121 cccgtcgggc gtcgcccggg ccgtggagga cgtgtctttt tcggtcgccc ggggcgagtt
      181 cctcgcgatc gtgggcggct cgggggcggg caagacgacc ctgctgcgca tgctggccgg
      241 gctgctggag ccgacctcgg gcgagatccg cttcaacggc cgggcggtgg cggggccgcc
      301 gaaggcattc gccgtcgtgt tccaggacta cgcccgctcg ctgtatccgt ggttcagcgt
      361 gcaccgcaac gtcgagctgc cgctggtcaa cctgattccc gacccgcgca agcgccgcgc
      421 ccgggtggac gacatgctcg agcgcgtagg cctgcacggc cacgcgcgca agcggccgca
      481 ccagttgtcg ggcgggcagc agcagcgcgt ggccatcgcg cgcgccctgg cctatgcccc
      541 cgacgtgctc atcatggacg aacccttcgc ctcggtcgac gcgcagacgc gcaatgagct
      601 ggaagacctg atgctcacgc tcaagcagga ttccggcatg accgtcgtct tcgtgaccca
      661 cgacgtggac gaggcggtct acatgagcga ccgggtgctg gtgctgggcc gcagtcccag
      721 cacggtggcc acggaagtcg gcatcgactt gccggcctcg cgcgaccaga tatcgaccaa
      781 ggccttgccg gctttcgcgg ccgcgcgaac cgccatcctg gtgtcgatcc gcgagcaggc
      841 ggcctcgggg cgcgccagtt ccctgccgcg ggccgcctga tccgcgggcc cgggctttcc
      901 tttttttcgt cccattcctc tttcggatat tttcatgaga cttttgcgtt tgtcctcctt
      961 ctccgcctgg gcggccgccc tcgccaccgc cgccgcgctc ggttcggccc cggcgttcgc
     1021 cgccgacgcg cccatcccgg tcaatatcgc cgtcatcccg acgctggacg ccggcccgct
     1081 gctgcttgcc aaggacaagg gcatcttcgc caggcacggc ctggacgtca ccatcaatgc
     1141 cggcggcacc ggcgcgacca tcatccccac cgtgctcagc cagcagtacg gcatcggcta
     1201 ttcgaacgtc atcagcgacc tggaggccat cgacaagggc ctgccgctgc tgctggtgca
     1261 cgccacctac ggccagcccg cggatcaggc ccgcgatccc tatcgggtgt atgtgagccc
     1321 gaacggcccg atcaagcaag ccaggcagct cgcccaggcg aacatcggca ccagctccat
     1381 ccagaacatt gcggaatgga cgacgcgcaa ggcgctggaa aacctgggcg tgaccgactt
     1441 cagcaagctg cgctggacca aggtggtcgg cgacgaggcc tacagcacgg tcaaggccgg
     1501 cacgctggac gcggtgtggc tgccgcagcc caccggcgcc gccgcggtgg ccgccgggct
     1561 ggtgcccatc ctgtcggcca attccctgtc gctgccgggg gccgtgggcg gttactacat
     1621 caccagcaag gcctatgcgc agagccatcc cgacgtgctt gcgcgcttca acgcctcgct
     1681 ggacgaagcc aatctctatg ccgccgcgca tccccaggaa ggcaaggaca tcgtggtcaa
     1741 ggtcctgcag ttcgaccaga agctggttga agccgccgac ctgaacgact acccgaacga
     1801 tccgggcgtc gacaagctga agatcatcgc cagcgatctc gtgcgctacg gcttcatcaa
     1861 gaaggcgccg gatccggacg ccatcttctg gaaggtgcgt tagaaaccgc gcgacgcgca
     1921 ctgcaacgcg cgggtccgcc aggcgtgggg cccgctccat tttttcaaga gagaacgaat
     1981 catggcagaa accaagactg aaacccagcc ggaaaccaag caagtccaag cccagaccat
     2041 cggactctcg ggccgctacc tgatcgatgc ggaaggcaat atcttcgtgt ccgattccgc
     2101 ctcgggccgc gaggaaggca ccgtcgcgcc ggagccgacc cacttcctgc tctccgcgct
     2161 ggccgtgtgc gccctgggca gcgtggaaaa ggaggcgcgc gagcgcggcg tgacggtggc
     2221 cggcggcacc gcgcaggtga cctcggtgcg cgacgagcac gaccgcacgc gctttgccaa
     2281 tatccagatc gacgtgaccg tgcgcggcgt gacgcaggcg caggcggaat tgctggtcgc
     2341 gcacttcacc ggcaattgcc ccatctacaa caccctgcgc cggggcggcc ccatcgccgt
     2401 cagcgtgcgc gccgaagcgt gaggagaggg tcatgacggc aatgggagcc tttcatgcct
     2461 aagcgcgcat tgcacgagct ggccaagccg gccgagcccg ccagcgtgcc cggcagcccg
     2521 ctggcccgcg tcctggccga acagcccttt ctgctgggca ttttcctgcc gctgcagaac
     2581 ggcgcctggt ctcaatcgac ctatccgcgc ggcacgggct ggacgttcga atacaaccgc
     2641 tcgctggtgc agcgcgcgga cgaactgggc ttcgaactcg gcttcggctt gtcgggctgg
     2701 ctgcccaagg gtgggcacgg cggcaggcag cgctatcgcg agaaccatct cgaccccttc
     2761 atcgccgcgg tgggcctggc ggccgtcacg cggcgcatga tcctggccgg gacgatccac
     2821 atcctctatc gctggcatcc gctgatgctg gccaagcagc tcgtcaccgc cgaccacgtg
     2881 gcgggcgggc ggttcggcgt gaacatcgtg accggcagtt ccgtggacgg cgcgcgcatg
     2941 ttcggcatcc agcgcgccga gcacgaccag cgctatcgcc aggccgacga gttcgtgcgc
     3001 ctgatgaagg atctctggca gggcgacgag gccctgagct acgacggttc ctggcccatg
     3061 gacgaggcct atatctcgcc tcgtccccgg tacggccgtc ccatcctgct ctcggctagc
     3121 agttcggccg ccgggttcga ctacggagcc gagcactccg acgtcgtctt tacctccagc
     3181 ccggtcggcg aacagttcga ggcgtcgatc gacgttctgc ccgcgcacgt ggcggacatc
     3241 aagcggcgcg cccgggagcg cggccgcagc gtgaaggtca tcatcaatgc gacggtcatc
     3301 accggccaga cggacgcgac ggcccaggcg tactacgacg ccatcctgga ccatgccgac
     3361 gacgaatcgc tggcctcgtt cgaccgcgag caccgctcca gcgacagcca ggcgtgggtc
     3421 aagcacgatg cccgcgcccg cgcggtgggc ggccacatcc acgtggtggg ctcctgggac
     3481 acggtggcgg acaagctgga gcgcctgcac gcggccggca tggacggtgt ccagctgacc
     3541 ttctacgact acctgccgga gatggagttg ttcgccaccc acgtcatccc ccggctcgag
     3601 gccaaaggcc tgcggcatcc ctacgtgcac cgctgagcct ttgcttttcc acttccagaa
     3661 tcacttgccg gtatgcctca cacacctttc catcttgcct ggttcatttc caagggctat
     3721 ggccccaagg cctggcgcca gccctggggc gggccgaacc cgcaccaatg ggtccacccg
     3781 gacttgttcg tcgacctggc gcgctccctg gagcgggccg ccttcgacta cgtcatgatc
     3841 gaggactcgt cgaacatccc ctacacctac gggaattcgc acgatgtcta cctgcgctat
     3901 gcggtcgact cgccgaagct ggacccggcc gtgctggcca cctacctgat ccaggccacg
     3961 cagcgcctgg gcgtggtcac cacgctttcc accagcgagt acaacccctt ccagctcgcc
     4021 cggctcacca ataccctgga ccacgtcagc cgcggacggg cgggctggaa cctcgtgacc
     4081 gggagcaatg acggcggggc gcagaacttc gggctggacc gccagtaccc gcatgaccag
     4141 cgctacgaca tggccgacga atacgccgaa ctggtcgtca agctgtggga gtcgtgggac
     4201 gccgacgcca tggtgatcga ccgggaggcc gggatcttcg ccgaccccgc caaggtccat
     4261 cccgtgcatt ttgagggcaa gttcttccgc tcgcgcgggc cgctggccgg gccgcgctct
     4321 ccccagggcc ggccggtcat ctgccaggcc ggcggttcgc cgcgcggccg ggtgttcgcc
     4381 tcgcgctggg ccgagaccat catcacgtcc gcgcgcagcg tggccgccat gaaggccttc
     4441 cgcgacgacg tccgcaaacg ggccgccgaa cagggccgca atcccgacga catcaagtgc
     4501 ctgttcctga tcaatcccat cgtcgaccag agccgcgagg gcgccgaggc gcggcgccag
     4561 gcgcagctgg cggacgccgc cagccatctg gagatgcacc tggcctcgct gtcgcgcacc
     4621 agcggcatcg acttctcggt ctacgacctg gacgagccgc tgcccgagct gaagtccaat
     4681 ggccaccaga cgctggtcgc ccaatacacg ggtcgcaccc tgcgcgagat cgccgccacg
     4741 ggcaagggct tgtcggacgt ggacctcgtc gggacgccgc gggatgtcgc cgccaagctc
     4801 gccgagatca tcgacgcggt gggcggcgac ggctttctct tcatggagcc cgagctgacc
     4861 cgccgctacg tggcggaaat cgccgatggg ctggtgccgg aattgcagcg tctgggcgcg
     4921 gtccgcaagc actacgagca cgagcggttc cgggacaatc tcctcgcgtt ctgaaaaggc
     4981 gcgtcaacgc cattgcgccg cggccgaacc caaggggctg gcggggtgtg cccagccggc
     5041 ccggcaaccc tgcagcgtga tcggccaggc gatgcgccgc gcgtcgcccc atacggtgcg
     5101 ctcgatctcg ggcgcctggt cggcggccgt ctccggcgcg aaggccggct cctgtggatg
     5161 gcccgtgccc tggtcgagca gcagcttggc ggtgcgtgcc agcgagagcc gcgcccgcag
     5221 ggcgccgccg cccagtcgcg cggccacgcc ccgcacggcc gcggcggcca gcagatagcc
     5281 cgtggcatgg tccagcgcct gcaccggcag cggacatggc ttgccggctt gcttccattg
     5341 catgcccgcg tgcgcgatgc ccgtgctcat ctgcatcagg ctgtcgaaac cgcggcgcgt
     5401 ggcccagggg ccgctccagc cgtaggcgtc caggctgacg tcgatcaggc cagggttgag
     5461 gcggcggcgc catgccgcgc cgaaccccag gcgctccagc gcgtcggacc ggtagccgtg
     5521 cagcatgatg tccgcgccgg cgagcagttg ttcgaacacg gcccggcctt gcggcgtgcg
     5581 caggtcgagc cgggcgcagc gcttgcccag cgtgacctcg ggcacggtgc cgggttcgtc
     5641 ccagtgcggc gggtcgatgc gcagcacctc ggcgccataa gcggccagga agcgggtggc
     5701 ggtcggcccg gccagcacgc gcgtcaggtc cagcactttc agcccggcga gcggccgggc
     5761 cgggtcgaca tcgcgatggc cgctgggcgc ggcatgtgtt tccatgtgga ccagcggctc
     5821 ggcggccaca gcctgcccct ggggatggcg cgaccattcc tcggcgctgc gcatttgcgc
     5881 cgcgcagccg ccagcctcga cgacggccgt ttccagttcg cgtttgtccc aggcggcgac
     5941 cgccgcggcc atgccggccc ggtcggtttg cctgcccagc acgcgctcgg ccgcggcgcg
     6001 gtggtgcggc gcgttggtgt gcaggcgtat ccagccgtcg cgcgtcgggt agtcgccggc
     6061 gacggggtcc cagaccggcg gcatctgcca gtcgatcgga cgcagcgacg tcgagaacca
     6121 gatcgaggcc aggcggcggt ccaccacgat gggggccgcc tggcggcccg cggcttgccg
     6181 cagcgtttcg atggcaagcg ccgcggccgc catggaagcc gcggccaggc cggtgaccgg
     6241 aaaggccgag ggcagggcgc ccgcggtggt gaatccaacc tgctccgggt cgcgggcgtt
     6301 gccgccgagc gaggcccata tcgatgcgag catgtccgag ctgatgtcca tgtcctgtcc
     6361 tcctgcatgg atgagtggat gcggttgacg ctacgatact tttcctgaaa ttgacgtcaa
     6421 tcttgatatc aactatcaat gttgatgaaa accatcaacc tggagcgctc gatgtcgtgg
     6481 ataccagccc gggcggcgct tgccctgctc gatgtgcggc cccagacgct gtatgccaat
     6541 gtcagccgcg gccgcatacg cgccaagccg gatccgcgcg acccgcgccg cagcctctat
     6601 catcgctccg atgtcgagcg cctggccggg cgccgatcgg ggcggcgcag cgcggcctcg
     6661 gtggcccagc aggccatgga gtggggcgat ccggtgctgg cttccggcat ttcgaccgtg
     6721 gagcacggcc ggctctggta ccgcggacgc gatgccgtcg aactggccga tacgcagacg
     6781 ctggaacacg tggcgggcct gttgtggaat gccgaaggca ttgctttcgg ggcgggccgg
     6841 caacgggcca ggtcgcgggc gcgtacggtt tcgccgctgg ccgcggcatt gtccacgctg
     6901 gccatgcgcg tcgatctcga tccgccgtcg cgcgggcggg cccggcaggc gctcattccg
     6961 gaagccgtgc gcctgctgga tgacctcatc ggcgccatgc tgggccgcgc gctcgaatcc
     7021 ggcatgcccg tgcatcggcg catggccgcc tactggcggg cgccgcgggc gcaggactgc
     7081 ctgcgtcgcg ccctggtgct cctggccgat cacgaactca atgcctcgac tttcgccacc
     7141 cgggtagccg tgtcgacggg gacgtcgctg tcggccagcc tgatggccgg cctggccacc
     7201 ttggccgggc cgctgcatgg cggcgcttcg ctgggcatcc tggcgttgcg cgcggccgcc
     7261 cagcgcgacg gggccgccgc cagcgtgcgg gaatacctgg ccagaggcga tttgctgccc
     7321 gcgttcgggc atcccctgta tcccgcagga gacccccggg ccgcggcgct attggcgcat
     7381 ttcaagctga ccaaggttta cgccgccttg cgcgacgaag ccatggcgct tacgggcgag
     7441 cagcccagca tcgatttcgc gctggcggcg gtgtccgacg ctctcgggct gcctgccgag
     7501 gcgccgttcg tgatctttgc cctggcccgt tccgtgggtt gggtcgccca tgcgctcgaa
     7561 cagatcgaga ccgggcatct gatccggccg cgagcccact atgtcggcgt gccgatcaca
     7621 tctgccgccg catgatcgct tgatcggcgg cgagctgtaa aagcggtttg taggtcggtc
     7681 aaggtggatt gcatcggttg ctccacctcg agatgtacga atagccgacc tcgccggctg
     7741 cggtcatcat cctggccggt agcgcgattt cttgccgatc aagcgttgtt tggatataca
     7801 attttcatgt atatccaaga acggagatgg acatgcaagt cgccaaatgg ggaaacagcc
     7861 tggccgttcg cttgccggcg gccttggtgg aggtgttgca attgcgcgaa ggtgacgaga
     7921 tcgagatcgt cgtggacgat ccacgcacat tttccgtgcg caagaaagcc gcggtcgatg
     7981 aactcatgga aagactgagg caattccggg gaataatgcc gccggatttc aaattcgacc
     8041 gggaagaggc caattcccgt ggccgataaa gtctttctcg acagcaatgt cattctgtat
     8101 tcgctgtcga gcgatcagga caaaatcagg gctgctgtgt ccttgctggc gcgcaggcca
     8161 acgatcagcg tgcaagtctt gaacgaggtc gcaaatgtat gcacgaccaa gctccgcatg
     8221 gcgtgggaca aaacagatct tttcctgcaa gaaatgcgcg acctttgtcg gatcatgccg
     8281 ttgaccatcg aagcacacga aaatgcggtc cagttggcgc agcgctatcg gttgccgttc
     8341 tacgacgcct gcatcgccgc gtcggcgcgt gcggcgaact gtcgggtttt gtacaccgaa
     8401 gacctgcatc atgggctggt cgtggaagac agcctgttga tccagaaccc gttcaagcgg
     8461 tagttgacgc aagctttggg tgccagggcg ggcaggcccc gccgtggcac ccaaaacgtc
     8521 ttaagaactc atcaatcaat ccagcttgcc acgctgcgtc tggacctttt ccagcgtctc
     8581 gc
//
LOCUS       DASWPO010000071        10460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2336999, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000071 DASWPO010000000
VERSION     DASWPO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336999"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VGC69_04335"
     CDS             complement(<1..394)
                     /locus_tag="VGC69_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY0294549.1"
                     /translation="MVSCVTGLPLVFEDEIKDWLDPPRYADLPESAPRASVDRLVAEA
                     RRLYPDNIVASVFVDDDEPQVFVLLVPSWEQAKADPASRHVVRFDAHTGAVLEQSKPP
                     GQQRQTFLGLMLHLHRDLFADLPGELFLG"
     gene            complement(533..3130)
                     /locus_tag="VGC69_04340"
     CDS             complement(533..3130)
                     /locus_tag="VGC69_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0294550.1"
                     /translation="MKEDHSESSADDGPLLAQPVLQGSRPMLCLPFRSRAINGDRLAL
                     PGQAPHASCKVPRTLTFALHCALTGIALVAPGMNAHAQARDFPGIATGVGVNHYEIPA
                     GPLSAGLNRLADQANVLLSASGTLTEGKNTPGLDGSYALGQAFSLLLTGTGLQAVRQN
                     DGTYSLRKIPESGAAAVMPAITVSGTAESAYGPGNGYVATQSATATKSSTPIIEVPQA
                     ISVVTRQQMDDQRPQTVASSLRYSSGVTSEAYGVASNYTDSYLYVRGFRPDVYLDGMK
                     QPPLSSTDPYFLERAELLHGPASVLYGQASPGGLVDLVSKRPVDTPLHEIEIGTGSYG
                     RAQTNFDFGGPLDQDGTLLYRLTGTAHTADTQVDDNKDRRIAIAPALTWHPDAATTLT
                     ILASYTHDPDLGAFSALPAIGTVLPSRYGQISRSFSIGDPGFNTWKRDQASIGYLLEH
                     RFDSVWTVRQNLRYSYLNSTERHLYGQGIGTDQRTISRYAYLDLNRRSSFDVDNQAEA
                     DFSTGPFAHKLLLGFDYQYLHVDETAQLGLAPSIDYLSPIYGVFSGGGPTLLSIPPYY
                     SKPVTMQRQAGLYTQDQIKWNNLSLLLGVRQDWATSDVSDSIANTTTHMADSATTWRT
                     GLVYEFANGIAPYIGYSTSFQPQSGTALGGAAFKPATGQQYEAGVKYQPAGYDSFITV
                     SAFNLTQQNVLTPDPVNTAYKVQTGEIRSRGFEIEGHANLTRNLSLIASYTYLANVVT
                     KANDSSLGKHPVAIPANMSSLWADYTFHSGALNGLGLSVGVRYVGKTYGTASNAWGIA
                     GYETAPSKVPGYTLFDAAVRYDFNRNWQLSVNASNLFDRKYVSSCLSASTCYYGPGRL
                     VLANIRYSW"
     gene            complement(3224..4231)
                     /locus_tag="VGC69_04345"
     CDS             complement(3224..4231)
                     /locus_tag="VGC69_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009914225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0294551.1"
                     /translation="MTAISSAALPDDEVKADYASLEEAAGWYATLHAAGSTDEQRQAW
                     AQWLEQRPEHRRAWRHVEAVSRRFEPMRTDGQREAAAAAVEVAMRGAMDRRLVLRGLA
                     ALAGTGWLGWLGWRHTNLPSLVTAWRSDYSTGVGEQRDLVLDDGTRVWLNTGSALDVA
                     YDGSQRLLTLAMGEILIDTAQDGQHRPFLVQTRFGRMQALGTRFTVRQNPDSTLLVVF
                     EGRVQIRNAAGDTQVVSAGQQRQFSASTIAAPGTADPARGAWTRGVVLAENIPLETLI
                     AELGRYQHGHLGVAPDVAKLRVVGRYPIDDPERALAMLENNFPVRVHRTLPWWITVEA
                     R"
     gene            complement(4243..4755)
                     /locus_tag="VGC69_04350"
     CDS             complement(4243..4755)
                     /locus_tag="VGC69_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0294552.1"
                     /translation="MPSQASTPNVEALYCDHHGWLQGWLQRRLGNATDAADLAHDAFL
                     RLLQTPRCFDSAPEARGYLRAMANGMCVDLWRRRQIERAWLDELAARPEPFEPSPEFR
                     AMVIETILEIGAVLGRLSLKAQQAFVMAQIHGCSTREIAAKLVVSERMVQKYLAQAML
                     QLMLVDAGLV"
     gene            complement(4909..6372)
                     /gene="rng"
                     /locus_tag="VGC69_04355"
     CDS             complement(4909..6372)
                     /gene="rng"
                     /locus_tag="VGC69_04355"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY0294553.1"
                     /translation="MNEDILINVTPFETRVAIVAQGAVQELHVERSIQRGYVGNIYLG
                     RVVRVLPGMQSAFVDVGLERAAFIHIADLRENRTERSQGQTPTPIEKLIFEGQTLMVQ
                     VIKDPLGTKGARLSTQISIAGRMLVHLPHDPHIGISQKIESETERTQLRERLQALIPA
                     EEKGGFIVRTQAEGATDEEISADLEYLRKLWVSVQEAARSHPVPALLHHDLTLSQRVL
                     RDMVGPQTGAILVDSRSTTVELQEWARRYTPSVADRIRHYSGERPLFDTANVDEEIAR
                     ALSRRVDLKSGGYLIIDQTEALTTVDVNTGGFVGGRNFDDTIFKTNLEASQAIARQLR
                     LRNLGGIVILDFIDMEDADHRDTVLAELKKALSRDRTRMTVNGFTQLGLVEMTRKRTR
                     DSLAHQLCEPCPMCQARGNVRTARTVCYEILREILRESRQFNPKEFRILASQDVVDLF
                     LEEESQHLAMLGDFVGKTVSLEVEATYSQEQYDIVLI"
     gene            complement(6447..7349)
                     /locus_tag="VGC69_04360"
     CDS             complement(6447..7349)
                     /locus_tag="VGC69_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294554.1"
                     /translation="MDVRALRYFVETVRCANFTQAARKLFVTQSTISKMIRQLETETG
                     AQLLIRDGHTARPTDAGRVVYERGVQVLDVMRQLSAELQETTQLERGTLEVGIPPMIN
                     VLFTPAVKRFRERYPHIALRLHEDTGRSVERQVASGELEVGATVLPLEPGSPLQIRRF
                     GNHAIWAIGKSGAPWNGKTMLPLSALAETPLILTGDDFALTRLLRQAFQDAGLQPRIA
                     AQSAHWDFLVSMASADLGVALLPEPLMRRMNTRGLSTAKLARTGLQWELAHIWQGQRY
                     LSHAARAWLEVCQETMGKAAHPAA"
     gene            7558..9075
                     /locus_tag="VGC69_04365"
     CDS             7558..9075
                     /locus_tag="VGC69_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HEY0294555.1"
                     /translation="MQVERIRHPALCDRVMSAREAARFVRDGMTVGMSGFTRAGDCKA
                     VPAALAERAASEPLRITLITGASLGHDTDKRLAQAGVLARRMPFQVDTTLRRMVNQGD
                     VAFIDQHLSETAEQLRAGHLQRVDVAFIEAAAITEDGGIVPTMSVGNSANFAEQADKI
                     IIELNVGVPTGIEGLHDIYVPGARPGREPIPLTDVSQRIGTTSIRVDPAKIVAIVITD
                     EPDSPSNALPPDAETGAIAGHINAFLKSEVRQGRLSHGLLPLQAGIGTIANAVLHGFA
                     ESGFEHLTMYSEVLQDSAIELIDRGCLDFASASSITVSEPVYQRLLDRMDHYRKHIVL
                     RPQEISNAPELVRRLGIIAINTALEVDIYGNVNSTHVGGTHMMNGIGGSGDFARNAHL
                     AIFVTKSVARGGDISSVVPMVPHVDHNEHDVDVIVTERGLADLRGLAPRERARAIVRH
                     CAHPAYRDALQDYFDRACATRGGQTPHLLREAFAWHERHEATGSMQPRLRRQQPA"
     gene            complement(9098..10081)
                     /locus_tag="VGC69_04370"
     CDS             complement(9098..10081)
                     /locus_tag="VGC69_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY0294556.1"
                     /translation="MQAIARFSRFVGNTFALWVLLFSVLAYVAPDSFKWLGAYIVPLL
                     GLVMFGMGLTLSRDDFRAVLRQPGVVAIGVVGHFAIMPTIAWALTKLLHLPPEIAVGV
                     ILVGCCPGGTASNVVSLLARGDIALSVAITAVTTLLAPIATPALIYFLAHQWLHVDAA
                     SMFWSIVQVVLVPIALGAAAQYLFRSRIQVFVQVLPIVSVAAIVIIVAAVVAGSRASL
                     AQSGLLIFLAVVLHNGFGLLLGYLLGKVFHLPVAQRKALSIEVGMQNSGLGVALAHAH
                     FSPLAAVPSAIFSVWHNISGALVATLYRRFHDAAPQAAQTAAPGASANSAG"
BASE COUNT         1908 a         3209 c         3431 g         1912 t
ORIGIN      
        1 cgcccaggaa cagctcgccg ggcaggtccg cgaacagatc acgatgcaaa tgcagcatca
       61 ggccgagaaa ggtctgcctt tgctggccgg gcggcttgga ctgctccagc accgcgccgg
      121 tgtgggcgtc gaagcgcacg acgtgccgcg atgccggatc cgccttggcc tgttcccaag
      181 agggcaccag caagacgaat acctggggct catcgtcgtc gacgaagacc gaagcgacaa
      241 tattgtcggg atagagtcgg cgcgcctcgg ccaccagccg gtcgacgctg gcgcgcggcg
      301 ccgattcggg caggtcggca tagcgcggcg gatcgagcca gtccttgatt tcgtcttcaa
      361 ataccagggg caggccggtt acgcagctga ccaacagaaa aatcgtgcag acgaggcttg
      421 tccatttatg tatggatacc caccaacgca gtggaacgga aaacataggg tctccctgtg
      481 aacgttcggg aatccgggtg gaggcgatcc aacgccacct gttcgcgcat cgtcaccagg
      541 aatatctgat gttggcgagc accaggcgcc ccgggccgta atagcaggtg ctcgcggaca
      601 agcaggaaga gacatacttt cgatcgaaga gattcgatgc attgacactc agctgccagt
      661 tcctgttgaa gtcatagcgg accgcggcat cgaacaatgt atagcccggc accttcgaag
      721 gcgcggtctc gtagcctgct atcccccatg cgttcgaagc cgtcccatat gtctttccga
      781 catatcgcac gccgacggat agtcccaatc cattcagggc gccggagtga aacgtatagt
      841 ctgcccaaag cgacgacatg tttgcgggaa tcgcgacagg gtgtttgccg agcgaactat
      901 cgtttgcttt ggtgacaacg ttggcgagat aggtgtagga agcgatcaag ctcagattcc
      961 tggtgagatt ggcgtgtcct tcgatctcga agccccggga ccggatttct ccagtctgaa
     1021 ccttatatgc ggtgtttaca ggatcgggcg tcaggacatt ctgctgtgtc agattgaatg
     1081 ccgacaccgt gatgaagctg tcatagccgg ccggctgata tttcacgccg gcttcatatt
     1141 gctggccggt ggccggtttg aacgcggcgc cgccgagagc cgttccggac tgtggttgga
     1201 atgaggtgga atagccgatg tagggcgcaa tgccgtttgc aaactcgtat accaggcccg
     1261 ttcgccatgt cgtggcgctg tcggccatat gggtcgtggt attggcgatg ctatcgctga
     1321 cgtcgctggt ggcccagtcc tgccgaaccc caagcagcaa ggaaagattg ttccacttta
     1381 tttgatcctg ggtgtacaac ccggcttgcc tttgcatggt gacaggcttt gagtaatagg
     1441 gcggtattga gagaagggtt ggtccgccgc cactgaaaac gccatatatc ggcgaaagat
     1501 agtcgatcga tggagcaagt ccgagttggg ctgtttcgtc gacatgcaag tactggtagt
     1561 cgaagccgag aagcagcttg tgcgcgaacg gcccggtcga gaagtctgct tcggcctgat
     1621 tgtcgacgtc gaacgatgac ctgcggttta gatcgaggta agcgtagcgg ctgatcgtcc
     1681 tctggtcggt accgatgcct tgtccatata gatgcctttc ggtcgagttg agatatgagt
     1741 agcgcaagtt ttgtctgact gtccagacgc tgtcgaagcg atgctcgagt agatagccaa
     1801 tggaagcctg gtcccgcttc caggtgttaa agccggggtc tccgatagag aagctgcggg
     1861 aaatctggcc atagcggctg ggcagtacgg ttccgatggc gggcagcgcg gagaatgcgc
     1921 caagatccgg atcatgggtg tagctggcca gaatagtcag cgttgtcgcg gcatcgggat
     1981 gccaagtcag cgcgggagca atggcgatcc gtcgatcttt gttgtcgtca acctgagtgt
     2041 ctgcggtgtg tgccgttccg gtcaggcggt aaaggagcgt accgtcctgg tcgagggggc
     2101 cgccgaagtc gaaattggtc tgcgcgcgcc cataggagcc agtgcctatt tcgatttcgt
     2161 gcagcggcgt gtccaccggc cgcttgctga cgagatcgac aagaccgccg gggcttgctt
     2221 gcccatacag aaccgatgcg ggaccgtgga gcagttcggc tctttccaga aagtaggggt
     2281 cggtcgagct cagcggcggc tgcttcattc catcgagata cacatcgggc ctgaagcccc
     2341 ggacgtaaag ataagagtcc gtatagttcg atgccacgcc ataggcctct gaggtcacgc
     2401 cggagctata gcgcaaagac gacgccacgg tttgcggccg ctgatcgtcc atttgctgcc
     2461 gcgtaaccac cgaaatggct tgcggaacct cgattatcgg cgtgctgctc ttggttgcgg
     2521 tcgcgctttg cgtggccacg tagccgtttc caggcccata ggcgctttct gccgttccgc
     2581 tcacggttat cgcgggcatg acagccgcag ccccggattc ggggattttc cgcagggaat
     2641 aagtcccgtc gttctgtcgt actgcctgca gtcccgttcc ggtcagcagc agcgagaagg
     2701 cctggcccag ggcataggag ccatccagtc ccggcgtgtt cttgccttcg gttagcgttc
     2761 ccgaggcgct cagcagcaca ttggcctgat cggcgagccg gttcaatccc gccgacagcg
     2821 ggccggccgg aatttcgtag tggttgacgc caactccggt ggcaatacca ggaaaatccc
     2881 gagcctgcgc gtgtgcgttc atacccggtg cgaccagcgc aatccccgtc aaagcgcagt
     2941 gcagcgcgaa ggtcagcgtc ctgggaacct tgcatgaggc atggggggcc tgtccgggca
     3001 aggccagacg atcgccattg atggcgcgcg atcggaacgg gagacaaagc atggggcgac
     3061 ttccctgaag tacaggttgg gcaaggagag gcccatcgtc agccgacgat tccgaatggt
     3121 cctctttcat ctacggaatc gaacgagatg aaagaaaacg aaccgatttc ggaaaatttt
     3181 ttacggacgc cagttcaacg cttggaaagt cccgtactgc cggtcatcgc gcttctaccg
     3241 ttatccacca gggcaaggtg cgatgcaccc gtacgggaaa gttattttcc agcatggcca
     3301 gcgcgcgctc gggatcgtcg atggggtaac gccccacgac tctcaacttg gccacgtccg
     3361 gcgccacccc cagatgcccg tgctggtagc ggcccagctc cgcgatcaac gtttcgagcg
     3421 ggatgttttc agccaagacg acgccccgcg tccatgcccc gcgcgcaggg tcggccgttc
     3481 ctggggccgc gatggtgctg gcactgaact gtcgctgctg gcctgcggag accacctggg
     3541 tatcgccggc cgcattgcgg atctggacac gaccctcgaa aaccaccagc aacgtcgagt
     3601 cagggttctg gcgcacggta aagcgggtgc cgagcgcctg catccggccg aaccgggtct
     3661 ggaccaggaa gggacgatgt tgcccgtcct gggccgtgtc gatgaggatt tctcccatcg
     3721 ccagggtcaa taggcgttga gagccgtcat aggcgacatc gagggcgctt ccggtgttca
     3781 gccagacgcg tgtgccgtcg tccagcacca ggtcgcgctg ttccccgacg cccgtgctgt
     3841 agtccgaacg ccaggccgtc accaggcttg gcaagtttgt gtggcgccat cccagccatc
     3901 ccagccagcc cgtgccggcc agagcggcca ggccgcgcag caccaggcgc cgatccatgg
     3961 cgccacgcat tgcgacttct accgccgccg ccgcggcttc ccgttgccca tccgtgcgca
     4021 tgggctcgaa acggcggctg acggcctcga cgtgccgcca cgcgcggcgg tgttctgggc
     4081 gctgctccag ccattgcgcc caagcctggc gctgctcgtc ggttgagccg gcggcatgga
     4141 gtgttgcgta ccacccagcc gcctcttcaa gactggcata gtccgctttt acctcgtcgt
     4201 cgggcagcgc cgcggaagaa atggcagtca tggtcgatct cgtcagacca gcccggcatc
     4261 gacgagcatc agctgcaaca tggcttgggc cagatatttc tggaccatgc gctcggatac
     4321 gaccagcttg gcggcaattt cccgcgtgga gcaaccatgg atttgcgcca tcacgaaagc
     4381 ttgttgagcc ttgagggaaa ggcgcccaag cacggcgccg atttccagga tcgtttcgat
     4441 caccatcgcc cggaactcgg gcgacggttc gaagggttcg ggccgtgcag ccagctcatc
     4501 gagccacgcc cgctcgatct ggcgacggcg ccagagatcc acgcacatgc cgttggccat
     4561 cgcgcgcagg taaccgcggg cttccggcgc actgtcgaag cagcgcggcg tctgcaacag
     4621 ccgcaggaag gcgtcgtggg cgaggtccgc cgcatcggtc gcattgccca agcggcgctg
     4681 cagccaccct tgcagccatc catgatggtc gcagtagagg gcctcgacgt tgggagtgga
     4741 tgcctggctt ggcacgatct tgtgcagggc gtcgacagaa agtccaacac caaatctaaa
     4801 tggtaattac tatcaattat ggttggaagc tcgggtttgt gcaacatggc gtgatcggga
     4861 acggatccgg accttatggc actgcgaaca gctggtcagg attcccgttt agatcaacac
     4921 gatgtcgtat tgctcttgag aataggtggc ttccacttcc agcgacaccg tcttgccgac
     4981 gaaatcgccc agcatggcca gatgctggct ttcttcctcc aggaacaagt ccaccacgtc
     5041 ctgtgaagcc aggatgcgga attccttggg attgaactga cggctctcgc gcaggatctc
     5101 gcgcaggatc tcatagcaga ccgtgcgcgc cgtgcgcaca ttgccgcgcg cctggcacat
     5161 ggggcaaggc tcgcacagct ggtgggccag cgaatcgcgc gtacgcttgc gggtcatctc
     5221 caccaggccg agctgggtaa agccgttgac cgtcatgcgg gtgcggtcgc gcgacaaggc
     5281 cttcttcagt tcggccagca ccgtatcccg gtggtcggca tcctccatat cgatgaagtc
     5341 caggatgacg atgccgccca ggttgcgcag ccgcagctgc cgggcaatgg cttgcgaggc
     5401 ttccagattg gtcttgaaga tggtgtcgtc gaagttgcgg ccgccgacga aaccgccggt
     5461 attcacgtcc accgtcgtca aggcctcggt ctggtcgatg atcaggtagc cgccggattt
     5521 caggtcgacg cggcgcgaca gggcccgggc aatttcttcg tcgacattgg cggtgtcgaa
     5581 gagcggacgt tcgccgctgt aatggcggat gcggtcggcg acggaggggg tatagcgccg
     5641 cgcccattcc tgcagttcca ccgtggtgct gcgcgaatcc accaggatcg ccccggtttg
     5701 cgggccgacc atgtcgcgca gcacgcgctg cgacagggtc aggtcatggt gcagcagggc
     5761 ggggacgggg tggctgcgcg cggcttcctg cacgctcacc cagagcttgc gcaggtattc
     5821 caggtcggcc gagatttctt cgtccgtggc gccttcggcc tgggtgcgca cgatgaaccc
     5881 gcctttttcc tcggcgggga tgagggcttg cagccgctcg cgcagctggg tgcgttccgt
     5941 ctcggactcg atcttttgcg agatgccgat gtgcgggtcg tgcggcaggt gcaccagcat
     6001 gcggccggca atgctgatct gcgtcgacag ccgggctccc ttggtgccga gcgggtcctt
     6061 gatgacctgc accatcaggg tctggccttc gaagatcagc ttctcgatcg gggtcggggt
     6121 ctggccctgg ctgcgttcgg tccggttctc gcgcaggtcg gcgatgtgga tgaaggcggc
     6181 gcgctccagc cccacgtcga cgaaggcgct ttgcatgccc ggcagcaccc gcaccacgcg
     6241 gcccagatag atgttgccca cgtagccgcg ctggatgctg cgctcgacat gcagttcttg
     6301 cacggcgcct tgcgccacga tggcaacgcg cgtttcgaag ggggtgacgt tgatcagtat
     6361 gtcttcgttc atggctcgtg ccgtctgcag gatggtttgc tgtctcgtag ggtaccgtaa
     6421 cccggggacg gatgcgtgcc ggcgggttac gcggccgggt gcgccgcttt gcccatggtt
     6481 tcctggcaca cctccagcca ggcccgggcc gcgtgggaga ggtagcgctg gccctgccag
     6541 atatgggcca actcccactg caggccggtt cgggccagct tggccgtgct caacccgcgg
     6601 gtgttcatgc ggcgcatcag gggttcgggc aacagcgcga cgcccaagtc cgccgacgcc
     6661 atggacacca ggaaatccca gtgcgcgctt tgcgcggcga tgcggggctg cagtcccgcg
     6721 tcctggaagg cctggcgcaa caggcgggtc agggcgaaat cgtcgccggt caggatgagc
     6781 ggcgtttcgg ccagggcgga aaggggcagc atggtcttgc cgttccaggg cgccccgctc
     6841 ttgccgatgg cccagatggc gtggttgccg aagcggcgga tctgcagggg gctgccgggt
     6901 tcgaggggga gcaccgtcgc gcccacttcc agctcacccg aggccacctg ccgctccacg
     6961 ctgcgcccgg tgtcttcatg caggcgcagc gcaatgtgcg ggtagcgttc gcgaaagcgc
     7021 ttgaccgccg gcgtgaagag cacattgatc atgggcggga tgcccacctc cagcgtgccg
     7081 cgctccagct gcgtggtttc ctgcagttcg gccgacagct gccgcatgac gtccagcacc
     7141 tggacgccgc gttcgtacac cacgcggccg gcatcggtgg ggcgggccgt gtgtccatcc
     7201 cggatcagca gctgggcgcc tgtttcggtt tccagctggc ggatcatttt gctgatggtc
     7261 gattgcgtca cgaacagctt gcgcgcggcc tgcgtgaagt tcgcgcaacg cacggtttcg
     7321 acgaaatagc gcagggccct gacatccatg atgaaaaaac cgactcaaat ttatgaaatt
     7381 aattcatttt acgacttgtt tctcttgtcc atagactccg cccattcagc gatccggctg
     7441 cccggcgggc aacccggacc gcgccattta ccgggaacgc cgcgcagacg gtgcccaggg
     7501 cagcccggca tgcctcgcgt cagccccgtg ccgggcgcgg actcaacagg agacaccatg
     7561 caagtcgaac gtatccgtca tcccgccttg tgcgatcgcg tcatgtccgc cagggaggcc
     7621 gcgcggttcg tgcgcgacgg tatgaccgtg ggcatgagcg gctttacccg cgccggcgat
     7681 tgcaaggccg tgcccgcggc cctggccgaa cgggcggcga gcgaaccgct gcgcatcacg
     7741 ctgattaccg gcgcctcgct ggggcacgac accgacaagc gcctggccca ggcgggcgtc
     7801 ctggcgcggc gcatgccctt ccaggtcgac accacgctgc ggcgcatggt caaccagggg
     7861 gacgtggcct tcatcgacca gcacctgtcc gagacggccg agcaattgcg cgcgggacat
     7921 ctgcagcgcg tcgacgtcgc gttcatcgag gctgcggcca ttaccgagga cggcggcatc
     7981 gtgccgacca tgtcggtcgg caattcggcc aatttcgccg agcaggccga caagatcatc
     8041 atcgagctca acgtcggcgt gcccacgggc atcgaaggcc tgcacgacat ctacgtcccg
     8101 ggcgcgcgtc ccggcaggga accgatcccg ctcacggacg tgtcgcagcg catcggcacg
     8161 acgagcatcc gggtcgatcc cgccaagatc gtcgccatcg tcatcacgga tgagcccgac
     8221 agcccgtcca acgccttgcc gcccgatgcc gaaaccggcg ccatcgcagg gcacatcaac
     8281 gccttcctga aaagcgaggt tcgccagggg cgcctgagcc atggcttgct gccgctgcag
     8341 gcgggcatcg gcaccattgc caacgccgtg ctgcatggct tcgcggagtc cggcttcgag
     8401 cacctgacca tgtattcgga ggtgctgcag gacagcgcca tcgagctgat cgaccggggg
     8461 tgcctggatt tcgcctcggc ctcttccatt accgtgtcgg agccggtcta ccaacgcctg
     8521 ctcgaccgca tggaccatta ccgcaagcac atcgtgctgc gcccgcagga aatcagcaac
     8581 gcgcccgagc tggtgcggcg gctgggcatc atcgccatca ataccgcgct ggaggtggac
     8641 atctacggca acgtcaattc cacccatgtg ggcggcaccc acatgatgaa tggcatcggc
     8701 ggctcgggcg atttcgcgcg caacgcccac ctggccatct tcgtgaccaa gtcggtggcg
     8761 cgcggcggcg atatctccag cgtcgtgccc atggtgccgc acgtcgacca caacgagcat
     8821 gacgtcgacg tgatcgtgac cgagcggggc ctggcggatc tgcgcggcct ggcgccgcgg
     8881 gagcgggccc gcgccatcgt ccggcactgc gcgcatcccg cctaccggga tgccttgcag
     8941 gattatttcg accgggcgtg cgccacccgg ggcggacaga ccccgcacct gctgcgcgag
     9001 gcctttgcct ggcacgagcg ccacgaggcc acgggcagca tgcagccgcg gctgcgccgc
     9061 cagcagccgg cctgatgcct cgggccggcg cgcgcgttca gcctgccgag ttcgcggaag
     9121 cgccgggggc ggccgtttgc gccgcctgtg gcgctgcgtc gtgaaagcgc cggtacagcg
     9181 tggccaccag cgcgcccgag atgttgtgcc acacgctgaa aatggcgctg ggcacggccg
     9241 ccagcggcga gaaatgcgca tgggccagcg cgacgcccag gcccgaattc tgcatgccca
     9301 cctcgatgga caaggccttg cgctgggcga ccggcaggtg gaacaccttg cccagcagat
     9361 agcccagcag caagccgaaa ccattgtgca gcaccaccgc gagaaagatc agcaggccgg
     9421 attgcgccag gctggcccgg ctgccggcca ccacggccgc cacgatgatg acgatggccg
     9481 ccaccgacac gatgggcagc acctgcacga acacctggat gcggctgcgg aacagatatt
     9541 gcgcggcagc gcccagggca atgggaacca gcaccacctg cacgatggac cagaacatgg
     9601 atgcggcgtc cacgtgcagc cattggtggg ccaggaagta gatgagcgcg ggcgtggcaa
     9661 tgggcgccag cagcgtcgtc accgcggtaa tggcgaccga cagcgcgatg tccccgcgcg
     9721 ccagcaggga cacgacattc gaggcggtgc cgccggggca gcatcccacc aggatcacgc
     9781 ccacggcgat ctccggcggc aggtgaagca gcttggtcag ggcccaggcg atggtgggca
     9841 tgatggcgaa gtggccgacc acgccgatgg cgacgacgcc aggctggcgc agcacggcgc
     9901 ggaagtcatc cctggacagg gtcaggccca tcccgaacat gaccaggccc agaagaggga
     9961 cgatataggc gcccagccac ttgaatgaat cgggcgcgac ataggccagg acggaaaaga
    10021 gcaagaccca gagggcaaag gtattgccga cgaagcggct gaagcgggcg atggcctgca
    10081 tggaatgaat cctccttttt gtttatgcgg gccatgcctg ccatcttggc ggcgcgtccc
    10141 gggtgggaga ggattctact gtcacggctg ggccatgcct tgggctggtt tgcgtgttga
    10201 aaagaaggcg tgctatattt acgggctcgg cagcagcgac atcctgtgtg gtttgacgca
    10261 gccgccgagg gcgagagagc aggccagcgg gtcggttgag cagggtggga cgttacccgg
    10321 gtttgacagt ctcaaaaaag cggttcataa tctcgtttct ctgcttctga cgcagcgaac
    10381 aggttgggcg aaagttcagc gggtttggcg gcgcgaagcg ggcaagaaga ggggagatag
    10441 gcgaagtcag ctggtctttc
//
LOCUS       DASWPO010000072         9674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1890919, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000072 DASWPO010000000
VERSION     DASWPO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890919"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1351)
                     /gene="argJ"
                     /locus_tag="VGC69_04375"
     CDS             complement(101..1351)
                     /gene="argJ"
                     /locus_tag="VGC69_04375"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY0294557.1"
                     /translation="MAVNLQIPAESDIFPVAGVEIGVTEAGIRKAGRRDLTVFRLAEG
                     SSVAGVFTRNRFCAAPVQVCQAHLAGPANGGAKNGGAIRALVINTGNANAGTGAPGVA
                     AAEQTCAALGKLLGVAAEQVLPFSTGVILEPLPVDRLTAALPAAIARLGANEWFNAAH
                     GIMTTDTQPKIHSRKADIGGKTLTFTGISKGAGMIRPNMATMLSFLATDAGIAQPLLA
                     KLAGRIADQSFNRITVDGDTSTNDSFILIATGKSGLAIESESDPAYAAVAQAMTAAAL
                     DLAQKIVRDAEGATKFITIRVDEAGTREEALKVAYSVAHSPLVKTAFFASDPNLGRIL
                     AAIGYAGIDDMDASKVKLWLGDVLVSNDGGRTPSYREEDGQRVMKQAEIAMRIALGRG
                     SVSETVYTCDFSHEYVSINADYRS"
     gene            complement(1561..2421)
                     /locus_tag="VGC69_04380"
     CDS             complement(1561..2421)
                     /locus_tag="VGC69_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEY0294558.1"
                     /translation="MRNVDENTITGIVLERHGHAANPRLRQIMTSLVEHLHAFARDVR
                     LTEEEWFQGIDFLTRVGHITDDKRQEFILLSDTLGLSMLVTALNNRKPPGCTESTVFG
                     PFFVEGAPHYENGDDVANGAKGEPCFISGKVVGIDGAPVADARIDVWQADSDGYYDVQ
                     YADAGSQARGQLRSRADGTFHFRSVTAESYPIPHDGPVGAMLEALGRHPWRPAHLHFM
                     IEAPGYERLITHVFRSGDQYLDSDAVFGVRASLIADWQRHEAGAAPDGTQMDAPFSTL
                     DFTFVLNQAA"
     gene            complement(2460..3473)
                     /locus_tag="VGC69_04385"
     CDS             complement(2460..3473)
                     /locus_tag="VGC69_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294559.1"
                     /translation="MIPVSLPGGNYYLCMAVSLACLSTLLTWVTRLAIDRQSRLWLRA
                     HRHLGPALMTALAVAGGLFPYQQMSLWLAAQRDTREAQARRTVLDQAQLIAGIAMPAG
                     TILLLDLPKRLDSFVQADFPQAVAVAGLQARQVFRHVRPGGGSLPARETWSVALAADQ
                     AAQGWTCSRSHRVELVIDHDKPRFDSCHLAAGNTLDGQPLPAGTWLAWRQASPPRWLL
                     SIEGSEAAQIEHLPLLKADVIVDGPSHIVSFEGLLAQETKLGDMIYPTGTRAGSALNV
                     PGAKPGDLLFSPPRGRSARRAGQSDVQSGNSVLQAPDGTVRLVLNNRQAGVLDVATMR
                     IGP"
     gene            complement(3523..4011)
                     /locus_tag="VGC69_04390"
     CDS             complement(3523..4011)
                     /locus_tag="VGC69_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006228035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0294560.1"
                     /translation="MTPAPDLETRAAPDDHHALRLWLRLLTCTNLIESDIRGRLRTEF
                     DTTLPRFDLMAQLQRAPKGMKMGELSRHMMVTNGNITGITDQLEKEGLVVRTKVESDR
                     RSSVLKLTPQGRKTFARMARAHEDWVKGMFDDMPEASRHVLFKVLGELKLQIVARQGE
                     AG"
     gene            complement(4011..4913)
                     /locus_tag="VGC69_04395"
     CDS             complement(4011..4913)
                     /locus_tag="VGC69_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0294561.1"
                     /translation="MTNDILYRQFDRAALDVQYNARATVPDVMVFLNEYTEQSRQARA
                     SLPCATGLAYGEDPDETLDVFPAAAGSASPAPVFVFIHGGYWRALGKDDSSSMAPAFT
                     RAGAVVVAVNYSLAPAVTLDRIVDQNRRALAWVHRNIARYGGDPARIHIGGSSAGGHL
                     AGMMLASGWQQAAGLPADLVKGALLLSGLYDLTPLVHTHINAWMRMSPADAERNSPQR
                     HAPASGCPIVVSYGSNETDEFKRQSRDYLQAWQARGYPGQLTLVPGTNHFDIVLELSK
                     PDSPLTRAVFAQMGLTSSPADTPT"
     gene            5074..6252
                     /locus_tag="VGC69_04400"
     CDS             5074..6252
                     /locus_tag="VGC69_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294562.1"
                     /translation="MRVVHARLLVAALWAAALAPALAQAEPVKVGIAYDTSGPFAALG
                     AEARAGFDLAIKQLGGKLGGQPTEFVQADMAGNPDQARQLATRFIQRDKIDFFTGPIG
                     SNVALAVGPALFAAKIPFLSNNPGPSQFAGAQCNPYWFGLSYQNDAFHEAAGKVAADH
                     GYKKVYAIAPDYPAGRDALAGFKRGYKTPVAEEIYTKLGQSDYAAELATLRAAKPDAL
                     YVFLPGAMGVNFIKQFVAAGLSSGIALVAPSFSADEDVIAAVGEPMLGIYNTAQWAAD
                     LDVPRNKAFVAAFRQEYGGRYPTVYAAQAYDVIMAIDAAVKQVGGKVSDRAAVVKALE
                     KADFSSVRGPFAYGKNHYPIQAYYVRQVAKDDQGRLTNKLVGKVFDRYQDVYVGECKM
                     "
     gene            6387..8735
                     /locus_tag="VGC69_04405"
     CDS             6387..8735
                     /locus_tag="VGC69_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEY0294563.1"
                     /translation="MKIVCLGGGPAGLYFGLLMKLQNPANEVTVVERNRPYDTFGWGV
                     VFSDATMQNLRQADPVSAKTIVDAFTHWDDIDIQYKGRSIRSSGHGFIGIGRKHMLNI
                     LQARCEEVGVKLVFETTVQDDQQAAIDYDADLVIASDGLNSLVRTRYADTFKPDIDTR
                     RCRFVWLGTTKVFDAFTFAFVETEHGWFQAHAYRFEDGMSTFIVETPEETWQAAGIEN
                     MSQEEAIAYCEQLFAAQLDGHRLISNATHLRGSAIWIRFPRVICNTWVRWNELGTPRG
                     QRRVPVVLMGDAAHTAHFSIGSGTKLALEDAIELARCLKDTSSVEEGLKRYEVVRSVE
                     VLKIQNAARNSTEWFENVARYANLEPEQFAYSLLTRSQRISHENLRLRDPAWLEGYEA
                     WIAAQAGVELPPGKRPPLPMLTPYKARGVTLPNRIVMSPMAMYSCTDGVPGDFHLVHL
                     GSRAMGGAGLVMVEMTCVSPDARITPGCPGLWTDEQGAAFKRIVDFVHGNSPARIGVQ
                     LGHAGRKGSTRLAWEGIDQPLESGNWPLVSASPLPYIEGVSQVPSAATRADMDRVRDD
                     FAAAARRAAAAGFDWLELHCAHGYLLSSFISPLTNQRGDEYGGSLENRLRFPLEVFRA
                     VRAAWPENQPMSVRISAHDLVEGGITADDAVAIARHFKAAGADMIDCSSGQVSRDEKP
                     VYGRMFQTPFADRVRNEAGIPTIAVGAIFEADHANGIIASGRADLCALARPHLADASW
                     TLRESARVGYRDVAWPKQYFAGKRQLETNFERAASFAKLDIK"
     gene            8770..9561
                     /locus_tag="VGC69_04410"
     CDS             8770..9561
                     /locus_tag="VGC69_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294564.1"
                     /translation="MDEPTQALAGRHALVTGGARGIGLACAKALLARGARVTLLGRGI
                     ESLQAAAASLNAPGRVGCAVADIADEASVQAAFAQAAAQFGPVHILVNNAGQASSQPF
                     ARTDARLWRDMLAVNLDGTFHCMQAALPGMLQAGWGRIINVASTAGLVGYAYVSAYVA
                     AKHGVIGLTRSVALEVAKKGITVNAVCPGYTETDIVRGAVANIVQKTGMSEDQARANL
                     AERNPQGKLVRPEEVADAVAWLALPSAGAVNGQSIAIDGGEVMTG"
BASE COUNT         1481 a         3240 c         3423 g         1530 t
ORIGIN      
        1 tcgatctcgg gcggcgcggc gggcagccag gcctcgagct gggccagcac gcgctcggcg
       61 cgttcgagca aatgagacag gttggaggaa tccataagga tcacgatcgg tagtcggcgt
      121 tgatgctgac gtactcgtgc gagaagtcgc aggtgtacac ggtctcgctc accgagccgc
      181 ggcccagggc gatgcgcatg gcgatctcgg cttgtttcat cacgcgctgg ccatcttctt
      241 cccggtagga aggcgtgcgg ccgccgtcgt tggacaccag cacgtcgccc agccacagct
      301 tgaccttgga ggcgtccatg tcgtcgatgc cggcatagcc gatggcggcc aggatgcggc
      361 ccaggttggg gtcggaagcg aagaaggcgg tcttgaccag cggcgagtgg gccaccgaat
      421 aggcgacctt gagcgcttct tcccgggtgc cggcctcgtc gacgcggatg gtgatgaact
      481 tggtggcgcc ctcggcgtcg cgcacgatct tctgcgccag gtcgagcgcg gcggccgtca
      541 tggcctgcgc cacggcggcg taggccgggt cggactcgga ctcgatcgcc aggcccgact
      601 tgccggtggc gatcaggatg aaggagtcgt tggtggaggt gtcgccgtcg accgtgatgc
      661 ggttgaagga ctggtcggcg atccggccgg ccagcttggc cagcagcggc tgggcgatgc
      721 cggcgtcggt ggccaggaag ctcagcatgg tggccatatt gggccggatc atgccggcgc
      781 ccttgctgat gccggtgaag gtgagggtct tgccgccgat gtccgccttg cgcgagtgga
      841 tcttgggctg cgtgtcggtg gtcatgatgc cgtgggcggc gttgaaccac tcgttcgcgc
      901 ccagcctggc gatggccgcg ggcagggcgg cggtgaggcg gtcgaccggc aggggctcga
      961 ggatcacgcc ggtggagaac ggcagcacct gttcggccgc cacgcccagc agcttgccca
     1021 gcgccgcgca ggtctgttcg gccgcggcca cgcccggcgc gccggtgccg gcgttggcgt
     1081 tgccggtgtt gatgaccagc gcgcggatgg cgccgccgtt tttggcgccc ccgttggcag
     1141 gcccggcgag atgcgcctgg cagacctgca cgggggcggc gcagaagcgg ttgcgcgtga
     1201 acacgcccgc cacgctgctg ccttcggcca ggcggaacac ggtgaggtcg cggcggccgg
     1261 ccttgcggat gcccgcttcg gtgacgccga tttcgacgcc ggcgacgggg aagatgtcgg
     1321 attcggcggg gatctgcagg ttgacggcca tgacttcggt ctcgtggtgg cagggtttgc
     1381 ggggtgggca agcgggcgcg gacgaccgcg cgggacggcc gccattatct caccgtctcc
     1441 atgccatgga tggcggtctc gctgcgctgc gcggcaaaaa aagcccccac gccgcgcctt
     1501 cggcctgctg cccctccgag ggggccgttt tcgccttggg gcggcccggc ggcaaaaaaa
     1561 tcaggcggcc tgattgagca cgaaggtgaa gtccagcgtg ctgaagggcg cgtccatctg
     1621 cgtgccgtcc ggcgcggcgc cggcttcgtg gcgctgccag tcggcgatca gcgaggcgcg
     1681 cacgccgaag acggcgtccg aatccaggta ttggtcgccg gagcggaaca cgtgggtgat
     1741 caggcgctcg tagccgggcg cctcgatcat gaagtgcagg tgcgcgggac gccaggggtg
     1801 gcggcccagc gcttcgagca tggcgcccac cggcccgtcg tgcggaatgg gataggactc
     1861 ggccgtgacg ctgcggaagt ggaacgtgcc gtctgcccgg ctgcgcagct ggccgcgggc
     1921 ctggctgccg gcatcggcgt actgcacatc gtagtagccg tcggagtcgg cctgccagac
     1981 gtcgatcctg gcgtcggcga cgggggcgcc gtcgatgccg acgaccttgc cgctgatgaa
     2041 gcagggctcg cccttggcgc cgttggccac gtcgtcgccg ttctcgtagt gcggcgcgcc
     2101 ctcgacgaag aaggggccga acacggtgga ctcggtgcag cccgggggct tgcggttgtt
     2161 gagcgcggtg accagcatcg acaggcccag cgtgtccgac agcaggatga actcctggcg
     2221 cttgtcgtcg gtgatgtggc cgacccgggt gaggaagtcg atgccctgga accactcttc
     2281 ttcggtcagc cgcacgtcgc gggcgaatgc gtgcaggtgc tcgaccaggc tcgtcatgat
     2341 ctggcgcagg cgcgggttcg cggcatgccc gtgccgttcc agcacgatcc cggtgatggt
     2401 gttttcatcg acgttgcgca tgaagcgtct cctggattat tggttttatt gtcggttttt
     2461 tacggcccga tacgcatggt ggcgacatcc agaacgccgg cctggcggtt gttgagcacc
     2521 aggcgcacgg tgccgtcggg cgcctgcagc accgagttgc cggactggac gtcgctctgg
     2581 ccggcgcgcc gcgccgagcg gccgcgcggc ggggaaaaca gcaggtcgcc cggtttggcg
     2641 ccgggcacgt tcagcgcgct gccggcccgg gtgccggtgg gatagatcat gtcgcccagc
     2701 ttggtttcct gggccagcag gccttcgaac gagacgatgt gcgacggccc gtcgacgatc
     2761 acgtcggcct tgagcagggg caggtgctcg atctgggcgg cttcgctgcc ctcgatgctc
     2821 agcagccagc gcggcgggct ggcctggcgc caggccagcc aggtgccggc gggcaggggc
     2881 tggccgtcga gggtattgcc ggcggccagg tggcagctgt cgaagcgcgg cttgtcatgg
     2941 tcgatgacca gttccacccg gtggctgcgc gagcaggtcc agccctgcgc ggcctggtcc
     3001 gccgccagcg cgaccgacca ggtctcgcgc gcgggcaggc tgccgccgcc ggggcggacg
     3061 tggcgaaaga cctgccgggc ctgcaggccg gccacggcga cggcctgggg gaagtcggcc
     3121 tgcacgaagg agtcgagccg cttgggcagg tcgagcagca ggatggtgcc ggccggcatg
     3181 gcgatgccgg cgatcagctg ggcctggtcc agcacggtgc ggcgggcctg ggcctcgcgg
     3241 gtgtcgcgct gggcggccag ccagaggctc atctgctggt agggaaagag gccgccggcc
     3301 acggccaggg cggtcatcag ggctggcccc aggtgcctgt gggcgcgcag ccacaggcgg
     3361 gattggcggt cgatggcaag ccgggtcacc caggtcagca gggtactcag gcaggccagc
     3421 gagaccgcca tgcagaggta gtaattgcca ccaggcaacg agaccgggat catggcggca
     3481 ttatgcgcca atccggtacc ggatcacgcg gcgcgcgccg gctcaaccgg cctcgccctg
     3541 gcgcgccacg atctgcagtt tgagctcgcc cagcaccttg aagagcacgt ggcggctggc
     3601 ctcgggcatg tcgtcgaaca tgcccttgac ccagtcctcg tgcgcgcggg ccatgcgggc
     3661 gaaggtcttg cggccctggg gggtgagctt gagcaccgag ctgcggcggt cggactccac
     3721 cttggtgcgc accaccaggc cctctttttc gagctggtcg gtgatgccgg tgatgttgcc
     3781 gttggtcacc atcatgtggc gcgagagctc gcccatcttc atgcccttgg gcgcgcgctg
     3841 cagctgggcc atgaggtcga agcggggcag cgtggtgtcg aactcggtgc gcaggcggcc
     3901 gcggatgtcg ctctcgatca ggttggtgca ggtcagcagg cgcagccaca ggcgcagggc
     3961 gtggtggtcg tcgggggcgg cgcgggtttc caggtcgggg gcgggggtca tcatgtgggg
     4021 gtgtcggcgg gggaggaagt caggcccatc tgcgcgaaga cggcgcgggt gagcgggctg
     4081 tcgggcttgc tgagctcgag gacgatgtcg aagtggttgg tgcccggcac cagggtcagc
     4141 tggccggggt agccgcgtgc ctgccaggcc tgcaggtagt cccggctctg gcgtttgaat
     4201 tcatcggttt cgttggatcc atagctgacg acgatggggc agccggacgc gggcgcatgg
     4261 cgctgcggac tattgcgctc ggcgtcggcc gggctcatgc gcatccatgc gttgatgtgg
     4321 gtgtgcacca gcggcgtcag gtcgtacagg ccggagagca gcagggcgcc cttgacgagg
     4381 tcggcgggca ggccggccgc ctgctgccag ccggaggcca gcatcatgcc ggccaggtgg
     4441 ccgccggccg aactgccgcc gatatggatg cgcgccgggt cgccgccgta gcgcgcgatg
     4501 ttgcgatgca cccaggccag ggcgcggcgg ttctggtcca cgatgcggtc cagcgtgacg
     4561 gccggcgcca gcgagtagtt cacggcgacc accaccgccc cggcccgggt gaaggccggc
     4621 gccatggagc tggagtcgtc cttgcccagg gcgcgccaat agccgccgtg gatgaagacg
     4681 aagaccgggg cggggctggc cgagccggcc gcggcgggga agacgtccag ggtttcgtcg
     4741 gggtcctcgc cgtaggccag gccggtggcg cagggcaggc tggcgcgcgc ctggcggctt
     4801 tgttcggtgt attcgttcaa gaacaccatg acgtcgggga cggtggcgcg cgcgttgtac
     4861 tgcacatcga gcgctgcgcg gtcaaattgg cggtaaagga tgtcgttggt cattccctgg
     4921 taaatccctg gtttttcttg caaatacttt aaatctaaac ttgtgcggca cgccagccgt
     4981 gagatcccgt gctggcggca cctcttgcgg ccgcgccgga aagcgcggcc cttccatccc
     5041 cgtccgctgc gctcgcgcgc aaggagatcg accatgcgtg ttgtccatgc ccgtttgttg
     5101 gttgccgccc tgtgggccgc cgccctggcg cctgccctgg cccaggccga gcccgtcaag
     5161 gtcggcatcg cgtacgacac gtccggcccc ttcgcggcgc tgggcgccga ggcccgcgct
     5221 ggcttcgacc tggccatcaa gcaactgggc ggcaagctgg gcgggcagcc caccgagttc
     5281 gtgcaggccg acatggccgg caatcccgac caggcccgcc agctcgccac gcgcttcatc
     5341 cagcgcgaca agatcgattt cttcaccggg cccatcggct ccaacgtggc cctggccgtg
     5401 ggcccggcgc tgttcgcggc caagattccc ttcctgtcca acaatcccgg ccccagccag
     5461 ttcgcggggg cgcagtgcaa tccgtactgg ttcggcctgt cctaccagaa cgatgccttc
     5521 catgaggcgg cgggcaaggt ggcggccgac cacggctaca agaaggtgta tgccatcgcg
     5581 ccggactacc cggccggcag ggatgccctg gccggcttca agcgcggcta caagacgccc
     5641 gtcgccgagg agatctacac caagctgggc cagagcgact atgccgccga gcttgccacg
     5701 ctgcgcgcgg ccaagcccga tgcgctctat gttttcctgc cgggcgccat gggcgtgaat
     5761 ttcatcaagc agttcgtggc ggccggcctg tcctccggca tcgccctggt ggcgccgagc
     5821 ttctcggccg acgaggacgt gatcgccgcc gtgggcgagc ccatgctggg catctacaac
     5881 accgcgcaat gggccgccga cctggacgtg ccgcggaaca aggccttcgt cgcggccttc
     5941 cgccaggaat acggcggccg ctaccccacc gtgtacgctg cgcaggccta tgacgtcatc
     6001 atggccatcg atgccgcggt caagcaggtg ggcggaaagg tgtccgaccg cgccgccgtg
     6061 gtcaaggcgc tcgagaaggc ggacttctcc tcggtgcgcg gacccttcgc ctacggcaag
     6121 aaccactatc ccatccaggc ctattacgtg cggcaggtgg ccaaggacga ccagggccgt
     6181 ctcaccaaca agctcgtcgg caaggtgttc gaccgctacc aggacgtgta cgtgggcgag
     6241 tgcaaaatgt agtagtcgca aggcatcaat atgcctgttg caggcggcgg ttttcgtggt
     6301 tattattagt tgaagcttaa aatatttagg ttggtgccag ccggctgcca tcgcgccggc
     6361 cgggcgccgc ccctacggag ttttccatga agatcgtctg cctgggcggt ggtcccgcgg
     6421 gcctgtattt cggcctgctg atgaagttgc agaaccccgc gaacgaggtg accgtggtcg
     6481 agcgcaaccg gccctacgac accttcggct ggggcgtggt gttttcggac gccaccatgc
     6541 agaacctgcg ccaggccgac ccggtttcgg ccaagaccat cgtcgacgcc ttcacccact
     6601 gggacgacat cgacatccag tacaagggcc gcagcatccg cagcagcggg cacggcttca
     6661 tcggcatcgg ccgcaagcac atgctcaata tcctgcaggc ccgctgcgag gaagtgggcg
     6721 tgaagctggt gttcgaaacc acggtgcagg acgaccagca ggccgccatc gactacgacg
     6781 ccgacctggt catcgcctcg gacggcctga acagcctggt gcgcacccgc tacgccgaca
     6841 ccttcaagcc cgacatcgac acgcgccgct gccgcttcgt gtggctgggc accaccaagg
     6901 tgttcgacgc tttcaccttc gccttcgtcg agaccgaaca cggctggttc caggcccacg
     6961 cctaccgctt cgaggacggc atgtcgacct tcatcgtcga aacgcccgaa gaaacctggc
     7021 aggccgccgg catcgaaaac atgagccagg aagaggccat cgcctattgc gagcagctct
     7081 tcgccgccca gctcgacggc cacaggctca tcagcaacgc cacgcacctg cgcggctcgg
     7141 ccatctggat ccgctttccg cgcgtgatct gcaatacctg ggtgcggtgg aacgaactcg
     7201 gcaccccgcg aggccagcgc cgcgtgcccg tggtgctgat gggcgacgcc gcccacaccg
     7261 cgcacttctc gatcggctcg ggcaccaagc tggccctgga agacgccatc gagctggccc
     7321 gctgcctgaa ggacacgtcc agcgtggaag agggcctgaa gcgctacgaa gtcgtgcgca
     7381 gcgtcgaagt gctcaagatc cagaacgccg cgcgcaattc gaccgagtgg ttcgagaacg
     7441 tcgcgcgcta cgccaacctc gagcccgagc agttcgccta ttcgctgctg acgcgttcgc
     7501 agcgcatctc gcacgaaaac ctgcgcctgc gcgacccggc ctggctggaa ggctacgaag
     7561 cctggatcgc cgcgcaggcg ggcgtcgagc tgcccccggg caaacggccg ccgctgccca
     7621 tgctcacgcc ctacaaggcg cgcggcgtca ccctgcccaa ccgcatcgtg atgtcgccca
     7681 tggccatgta ctcgtgcacc gacggcgtgc ccggcgactt ccacctggtg cacctgggca
     7741 gccgcgccat gggcggcgcg ggcctggtca tggtggagat gacctgcgtc tcgccggacg
     7801 cccgcatcac cccgggatgc cccggcctgt ggacggacga gcagggcgcg gccttcaagc
     7861 gcatcgtcga cttcgtgcac ggcaacagcc ctgcgcgcat cggcgtgcag ctgggccacg
     7921 cgggccgcaa gggttccacg cgactggcct gggaaggcat cgaccagccc ctggagagcg
     7981 gcaactggcc gctcgtctcg gcctcgccgc tgccctatat cgagggcgtc tcgcaagtgc
     8041 ccagcgccgc cacgcgcgcc gacatggacc gcgtgcgcga tgatttcgcg gccgccgccc
     8101 gccgcgccgc cgccgccggc ttcgattggc tcgaactgca ttgcgcccac ggctacctgc
     8161 tgtcgtcctt catctcgccg ctgaccaacc agcgcggcga cgaatacggc ggcagcctgg
     8221 aaaaccgcct gcgcttcccg ctggaggtct tccgcgccgt gcgcgccgcc tggcccgaaa
     8281 accagcccat gtcggtgcgc atctcggcgc acgacttggt cgaaggcggc atcaccgccg
     8341 acgacgccgt ggccatcgcc cgccacttca aggccgccgg cgccgacatg atcgactgct
     8401 cgtcgggcca ggtgagccgc gacgaaaaac ccgtgtacgg ccgcatgttc cagacgcctt
     8461 tcgccgaccg cgtgcgcaac gaggccggca ttcccaccat cgccgtgggc gccatcttcg
     8521 aggccgacca cgccaacggc atcatcgcct cgggccgcgc cgacctctgc gccctggccc
     8581 gcccgcacct ggccgacgcc tcctggacgc tgcgcgaaag cgcccgcgtg ggctaccgcg
     8641 acgtggcctg gcccaagcag tacttcgccg gcaagcgcca gctcgaaacc aacttcgagc
     8701 gcgccgcttc cttcgccaag ctggacatca aataggcgag ccgacatgag cgacgtgacg
     8761 gatgaccgga tggatgagcc gacgcaagca ctggccggcc ggcacgccct ggtcacgggc
     8821 ggggcgcgcg gcatcggcct ggcctgcgcg aaagcgttgc tggcacgcgg cgccagggtc
     8881 acgctactgg gccgcggcat cgaatcgctg caagccgcag cggcttcgct gaacgccccg
     8941 ggccgcgtgg gctgcgcggt cgccgacatc gccgacgagg cttcggtaca ggccgccttc
     9001 gcccaggccg cggcgcagtt cggcccggtg cacatcctgg tcaacaacgc cggccaggcc
     9061 agcagccagc ccttcgcccg caccgacgcc aggctgtggc gcgacatgct ggccgtcaat
     9121 ctcgacggca ccttccactg catgcaggcg gccctgcccg gcatgctgca agcaggctgg
     9181 ggccgcatca tcaacgtcgc cagcacggcc ggcctggtgg gctatgccta tgtcagcgcc
     9241 tacgtggccg ccaagcacgg cgtcatcggc ctcacccgct cggtggctct cgaggtcgcc
     9301 aagaaaggca tcaccgtcaa cgccgtgtgc cccggctaca ccgagaccga catcgtgcgc
     9361 ggcgccgtgg ccaacatcgt gcagaaaacc ggcatgagcg aagaccaggc gcgcgccaat
     9421 ctagccgaac gcaaccccca aggcaaactg gtgcggcccg aagaagtcgc cgacgccgtc
     9481 gcctggctgg ccctgccgtc cgccggcgcc gtcaacggcc agtccatcgc aatcgacggc
     9541 ggcgaggtaa tgacaggctg aagaattgga accattgcaa gtccgttcgg cgggcaagcc
     9601 cctgccggcc ctttgaggcg ggggcagccc ccgcaggggg ccgagggcgc ctgatggcgc
     9661 gcccgagccc cgcc
//
LOCUS       DASWPO010000073         4978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2694498, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000073 DASWPO010000000
VERSION     DASWPO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694498"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGC69_04415"
     CDS             <1..330
                     /locus_tag="VGC69_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0294565.1"
                     /translation="GNYDYEHKYFSDDTQYFCPADLPAGLAAEIQDIVVRAYRALGCE
                     GWGRADLILDRDNRPWLLELNTSPGMTGHSLVPMAARAAGMSYADLCVTILSEASCKL
                     RSPAREG"
     gene            343..1164
                     /locus_tag="VGC69_04420"
     CDS             343..1164
                     /locus_tag="VGC69_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY0294566.1"
                     /translation="MWNDARTINLIANTLAVLAVAAMLVAGLAWVAQRPYFALRGIEL
                     ESAPDSPLRYVSTGSVRSGIAGHLSGNFFTVDLNQARSLFESVPWVRHASVRRIWPNI
                     LRVRIEEQQPLALWNENQMINTWGEAFTANMGELDDDSDLPQFSGPEGSEELVVQRYA
                     ELARWFAPLNQHVTELDLSPRYAWRATLSTGMVLDLGRDPAADAPDPLGLPGALPFAA
                     RIQRFVQAWPAVQGKLEGREVMQADLRYPNGFALALAPLPETHSTKPDSKSSKKR"
     gene            1169..2395
                     /gene="ftsA"
                     /locus_tag="VGC69_04425"
     CDS             1169..2395
                     /gene="ftsA"
                     /locus_tag="VGC69_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0294567.1"
                     /translation="MTRDIKDLIVALDIGTSKVVAVVAEILPEGRFEVLGLGQHESRG
                     MRKGVVVNIESTVNSIQRALEEAELMADCKIRDVYTGIAGSHIRSFNSSGMVAVKDKE
                     VTATDVARVIETAKAVNIPTDQQVLHVLTQEFIVDGQEDIREPIGMSGLRLEVRVHIV
                     TGAVSAAQNIVKCVRRCGLEVQDLILQPLASSLACLTSDEKELGVVLVDIGGGTTDVA
                     IFTGGAIRHTAVIPIAGDQITNDIAAMLRTPTPDAEEIKLRYGVAKQVLASPDETVEV
                     PGLGDRGPRQVKRQALGAVIEPRIEELFNLVQQVVRESGYEDLLASGVVLTGGTAQLP
                     GMIELAEDVFLKPVRVAVPEYEGSLADVMHNPRFSTVMGLLQEARMQRLRGRKVATQA
                     GSFKSVLARMKEWFMN"
     gene            2589..3767
                     /gene="ftsZ"
                     /locus_tag="VGC69_04430"
     CDS             2589..3767
                     /gene="ftsZ"
                     /locus_tag="VGC69_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0294568.1"
                     /translation="MMNFEMLDSSTKGTVIKVVGVGGAGGNAVSHMIRSGVSGVDFIC
                     ANTDAQALAATNAAVQIRLGRTGLGAGAKPEQGRAAAETAREEIRAALNGAHMVFITA
                     GMGGGTGTGAGPVVAEVAKELGILTVGVVTKPFTFEGNKRLKMAEDGIAELGKHVHSL
                     IVVLNENLYDLMDEDATQEDCFKSADDILHNACAGIAEIINVEGNVNVDFEDVKTIMG
                     EQGQAMMGTATASGADRARVAAEKAIACPLLEGVDLNGARGVLVNITASRSLKMRETR
                     EIMETIRSYASDDATVIFGTAYDESMGENLRVTVVATGLGKAAARPQLVQTSAVDLRT
                     GTDNVAFVAAPQQGDYRGMDVPSVMRNPRSHATAQVRALESSGMDHFDIPAFLRKQAD
                     "
     gene            3987..4910
                     /gene="lpxC"
                     /locus_tag="VGC69_04435"
     CDS             3987..4910
                     /gene="lpxC"
                     /locus_tag="VGC69_04435"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY0294569.1"
                     /translation="MFRQRSIQNLVRTTGVGVHSGRRVEITLRPAEANTGIVFHRVDL
                     PEVVDLPARADGVGDTRMASVLQNGNVRVSTVEHLMSALAGLGIDNLHVDLTAEEVPI
                     MDGSAATFVYLLRSAGIVEQNAPKRFIRVLKPIEVREGEGPDAKWARLEPHEGFALAF
                     SIDFQHPAIDSTANFAEIDFATHSYAREIARARTFGFVNEVEALRSMGLARGGSLDNA
                     IVMDEYRVLNSDGLRYDDEFVKHKILDAIGDLYLLGKPLVARYVANKSGHALNNLLAR
                     TLMADQAAWEQVTYESQAEAPQAYRHEWKLA"
BASE COUNT          915 a         1638 c         1649 g          776 t
ORIGIN      
        1 ggcaactacg actacgagca caaatacttt tccgacgaca cgcagtattt ctgcccggcc
       61 gacctgccgg ccggcctggc cgcggaaatc caggacatcg tcgtgcgcgc ctaccgcgcg
      121 ctgggctgcg aaggctgggg ccgcgccgac ctcatcctcg accgcgacaa ccggccctgg
      181 ctgctggaac tgaacacctc gcccggcatg accggccatt cgctggtgcc gatggcggcg
      241 cgcgccgcgg gcatgagcta tgccgacctg tgcgtgacca tcctgtccga ggcgtcgtgc
      301 aagctgcgca gcccggcgcg ggaaggctga agggagccct aagtgtggaa cgacgctcgc
      361 accatcaacc tgatcgccaa cacgctggcc gtgctggcgg tggcggccat gctggtcgcc
      421 gggttggcat gggtggcgca acggccgtat ttcgcgctgc gcggcatcga gctggaatcg
      481 gcgccggaca gcccgctgcg ctatgtgtcg accggctcgg tgcgctccgg catcgccggg
      541 cacctgagcg gcaacttttt taccgtcgac ctcaaccagg cgcgctccct gttcgagtcg
      601 gtgccctggg tgcggcacgc ctcggtgcgg cgcatatggc ccaacatcct gcgcgtgcgc
      661 atcgaagagc agcagccgct ggcgctgtgg aacgagaacc agatgatcaa cacctggggc
      721 gaggcgttca ccgccaatat gggcgagctg gacgacgact cggatctgcc gcagttctcc
      781 gggcccgagg gcagcgagga gctggtggtg cagcgctatg ccgagctggc gcgctggttc
      841 gcgccgctca atcagcacgt gaccgagctg gacctgagcc cgcgctatgc ctggcgcgcc
      901 accctgtcca ccggcatggt gctggacctg ggccgcgatc cggcggccga tgcgcccgat
      961 ccgctgggcc tgccgggggc gctgccgttc gccgcgcgca tccagcgttt cgtccaggcc
     1021 tggccggccg tgcagggcaa gctcgagggc cgcgaggtga tgcaggccga cctgcgctat
     1081 ccgaacggct ttgcactggc gctcgcgccg ctgccggaaa ctcattcaac caaacccgac
     1141 tctaaatcct ccaagaagcg ataacgccat gacccgtgac atcaaggacc tcatcgtcgc
     1201 cctcgacatc ggcaccagca aggtggtcgc cgtcgtggcc gagatattgc cggaggggcg
     1261 gttcgaagtg ctgggcctgg gccagcatga atcgcgcggc atgcgcaagg gcgtggtggt
     1321 caatatcgaa agcacggtca actcgatcca gcgcgcgctg gaagaggccg agctgatggc
     1381 cgactgcaag atccgcgacg tctacaccgg cattgccggc agccacatcc gcagcttcaa
     1441 ctccagcggc atggtggccg tgaaggacaa ggaagtcacc gccaccgacg tggcccgcgt
     1501 gatcgagacg gccaaggcgg tgaacatccc caccgaccag caggtgctgc acgtgctgac
     1561 gcaggagttc atcgtcgacg gccaggaaga catccgcgag cccatcggca tgagcggcct
     1621 gcgcctggaa gtgcgggtgc acatcgtgac cggcgcggtg agcgccgcgc agaacatcgt
     1681 caagtgcgtg cgccgctgcg gcctggaagt ccaggacctg atcctgcagc cgctggcctc
     1741 cagcctggcc tgcctgacct ccgatgaaaa ggagctgggc gtggtgctgg tggatatcgg
     1801 gggcggcacc accgacgtgg cgattttcac cggcggcgcc atccgccata cggcggtgat
     1861 cccgatcgcg ggcgaccaga ttaccaacga tatcgccgcc atgctgcgca cgcccacgcc
     1921 ggacgcggaa gaaatcaagc tgcgctacgg cgtggccaag caagtgctgg caagccccga
     1981 tgagaccgtc gaggttcccg ggctgggcga ccggggcccg cgccaggtca agcgccaggc
     2041 gctgggcgcg gtgatcgagc cgcgcatcga agagctcttt aacctggtgc agcaagtcgt
     2101 gcgcgaatcc ggctacgaag acctgctggc gtcgggcgtg gtgctcaccg gcggcaccgc
     2161 ccagttgccc ggcatgatcg agctggccga agacgtgttt ctcaagcctg tgcgcgtggc
     2221 cgtgcccgaa tacgagggca gcctggccga cgtgatgcac aacccgcggt tttccacggt
     2281 gatgggcctc ctgcaagagg cccgcatgca acgcctgcgc ggccgcaagg tggcgaccca
     2341 ggccggcagc ttcaagtcgg tgctggcgcg catgaaggaa tggttcatga attaagcggg
     2401 aagcggcggg tttctaggcc tgccgggagc gagcccaagc cgaagcggcg cggacggact
     2461 ccaaagaggc gcttcaaaac cggtgcggat caggagatcg gcacctgctt tgaggcgatt
     2521 cacaaactag taggggaatg ttttcttact tgtgatttgc cttatggaca gatgagggag
     2581 tagtcatcat gatgaatttc gagatgcttg acagcagtac caaagggacc gtcatcaagg
     2641 tggtcggcgt gggcggggcg ggcggcaacg ccgtctcgca catgatccgc agcggcgtga
     2701 gcggcgtgga tttcatctgc gccaataccg acgcgcaggc gctggccgcg accaacgccg
     2761 cggtgcagat ccgcctgggc cgcaccggcc tgggcgccgg cgccaagccc gagcagggcc
     2821 gcgccgccgc cgaaacggca cgcgaggaaa tccgcgcggc gctcaacggc gcgcacatgg
     2881 tgttcattac cgctggcatg ggtggcggca ccggcacggg cgccggcccc gtggtcgcgg
     2941 aagtggccaa ggagctgggc atcctgacgg tgggcgtggt gaccaagcct ttcacgttcg
     3001 aaggcaacaa gcgcctgaag atggccgaag acggcattgc cgaactgggc aagcacgtgc
     3061 attcgctcat cgtggtgctc aacgagaacc tctacgatct gatggacgag gacgccaccc
     3121 aggaagactg cttcaagtcg gccgacgaca tcctgcacaa cgcctgcgcc ggcatcgccg
     3181 aaatcatcaa cgtcgagggc aacgtcaacg tcgacttcga agacgtcaag acgatcatgg
     3241 gcgagcaggg ccaggccatg atgggcacgg ccaccgccag cggcgccgac cgcgcccgcg
     3301 tcgcggccga gaaggccatt gcctgcccgc tgctggaagg cgtggacctg aacggcgccc
     3361 gcggcgtcct ggtgaacatc accgccagcc gctcgctgaa gatgcgcgaa acccgcgaaa
     3421 tcatggagac catccgcagc tacgcttcgg acgacgccac ggtgatcttc ggcacggcct
     3481 acgacgagtc catgggtgaa aacctgcgcg tgaccgtggt tgccaccggc ctgggcaagg
     3541 ctgcggcccg cccgcaactg gtgcagacct cggccgtcga cctgcgcacg ggtaccgaca
     3601 acgtggcgtt tgttgccgcg ccgcaacagg gtgactaccg cggcatggac gtgccttcgg
     3661 tgatgcgcaa cccgcgcagc cacgcgacgg cccaggtgcg cgcgctggaa agctcgggca
     3721 tggaccactt cgacatcccg gctttcctgc gcaagcaggc cgactaagcc tgtcgggccc
     3781 cggtttgcgg ggcggtacct ccctcaatct gcggcccgcc tccccgggcg ggccgcaggc
     3841 ggagcgtctg ccgtagcagg cgatttaagt gccgtagcgg taataatgtg aagtatttgc
     3901 aaggaaaagg ggacgtactg atggcgcaag ccgcgccgcc cggtttacaa taaagcctta
     3961 caaaaatatt aaacttgcct ctaactatgt tccgtcagcg cagtatccaa aatctcgttc
     4021 gtacgaccgg cgtgggtgtc cactccgggc gccgggtcga gatcaccctg cgcccggccg
     4081 aagcgaatac cggcatcgtg tttcaccgcg tcgacctgcc cgaggtcgtc gacctgcccg
     4141 cccgcgcgga cggggtgggc gacacccgca tggcgtcggt gctgcaaaac ggcaatgtgc
     4201 gcgtttccac ggtcgaacac ctgatgtcgg cgctggccgg cctgggcatc gacaacctgc
     4261 acgtcgatct taccgccgaa gaagtgccca tcatggatgg cagcgcggcc accttcgtgt
     4321 acctgctgcg ttcggccggc atcgtcgagc agaacgcgcc caagcgcttc atccgcgtgc
     4381 tcaagcccat cgaggtgcgt gaaggcgagg gccccgacgc caagtgggcc cgcctggaac
     4441 cgcatgaagg cttcgccctg gcgttctcca tcgacttcca gcacccggcc atcgattcca
     4501 cggccaactt cgccgaaatc gacttcgcca cgcattccta tgcccgcgag atcgcccgcg
     4561 cgcgcacctt cggcttcgtc aacgaggtcg aggcgctgcg ttccatgggc ctggcccgcg
     4621 gcggcagcct ggacaacgcc atcgtcatgg acgagtaccg cgtgctcaac agcgacggcc
     4681 tgcgctacga cgacgagttc gtcaagcaca agatcctcga cgccatcggc gacctgtacc
     4741 tgctgggcaa gccgctggtg gcccgctatg tggccaacaa gtcgggccac gcgctcaaca
     4801 acctcctggc ccgcaccctg atggccgacc aggccgcctg ggaacaggtc acctacgagt
     4861 cgcaggccga agcgccgcag gcctaccgcc acgagtggaa gctggcgtaa aaagcctggc
     4921 ccgatgaaaa acgccgctga tgcggctttt tttttatggc atggagaggc tggaagca
//
LOCUS       DASWPO010000074        11162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2694701, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000074 DASWPO010000000
VERSION     DASWPO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11162
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694701"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..633)
                     /locus_tag="VGC69_04440"
     CDS             complement(145..633)
                     /locus_tag="VGC69_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY0294570.1"
                     /translation="MPSLIKRRSFPSRPLFQPLLAAVLLAAPLLAAAQGQQAQGPQPQ
                     LPVIQLAAGIHVIHAEVADTNSERERGLMFRPALPGNDGMLFVFEVPDQQCFWMHNTP
                     RPLSIAFMADDGTIVNLENMAAETDDSHCSRKPVRYALEMAQGWFVGKGIKAGDRIDG
                     LP"
     gene            complement(708..953)
                     /locus_tag="VGC69_04445"
     CDS             complement(708..953)
                     /locus_tag="VGC69_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0294571.1"
                     /translation="MSDTTASAVPGNNPKATGTVKWFNDAKGFGFITPDDGGEDLFAH
                     FSSIQMNGFKTLKEGQKVSFEIIQGPKGKQALNITAA"
     gene            1179..1493
                     /gene="clpS"
                     /locus_tag="VGC69_04450"
     CDS             1179..1493
                     /gene="clpS"
                     /locus_tag="VGC69_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006383989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY0294572.1"
                     /translation="MSSTLDTQNDQVVEKAPAKTAPPPMYQVVLLNDDYTPMEFVVKV
                     LQKFFGKSHEEATRIMLQVHHEGRGICGVYPRDVAATRIAQVSQYARARQHPLQCVME
                     PV"
     gene            1780..4089
                     /gene="clpA"
                     /locus_tag="VGC69_04455"
     CDS             1780..4089
                     /gene="clpA"
                     /locus_tag="VGC69_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY0294573.1"
                     /translation="MISQELEVSLHMAFVEARSARHEFITVEHLLLSLLDNASAVEVL
                     RACAANLDDLRRNLRQFVTENTPVIPSGSEVDTQPTLGFQRVIQRAIMHVSAGGTGKK
                     PVTGANVLVAIFGEKDSHAVYYLQQQGVTRLDVVNFLSHGITKQPQEEPAAPQKEQQA
                     SAEEQGGEGRQSPLDQFATDLNAAALAGRIDPLIGREHEVERVIQVLCRRRKNNPLLV
                     GEAGVGKTAIAEGLAWRITRGEVPEVLQTSQVFALDMGALLAGTKYRGDFEQRLKGVL
                     KQVKANPDAILFIDEIHTLIGAGSASGGTLDASNLLKPALSSGQLKCIGATTYNEYRG
                     VFEKDHALSRRFQKIDVPEPSVEQTVQILRGLKSRFEEHHNVRYSAAALSAAAELAAR
                     YINDRHLPDKAIDVIDEAGAAQRLLAASKRKKVIGKAEIEAIVSKIARIPPQSVSNDD
                     RSKLATLDRDLKTVVFGQDQAIEALSAAIKMARSGLGKPDKPIGAFLFSGPTGVGKTE
                     VAKQLAFTLGVELLRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAITKQPHCVL
                     LLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAETLNRPTIGF
                     ANSREAGDEMVEIKRMFTPEFRNRLDAIIPFGSLTRDIILRVVDKFLMQLEDQLHQRR
                     VEAVFTERLRAYLAKTGFDPVMGARPMQRLIQDTIRRALADELLFGKLVDGGTVTVDV
                     DEADKVVLSFEDGKPTGSSAPPEEVALAD"
     gene            4093..5361
                     /locus_tag="VGC69_04460"
     CDS             4093..5361
                     /locus_tag="VGC69_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HEY0294574.1"
                     /translation="MDDQPLIACEHCASIYRRRVLRHGELAACTRCGTVLWRYSRLTL
                     NEWLAIALAGLLLFCIANAYPVARIEVYGAERGATLLDAVFITWQQHHWLVSIMTGLA
                     GFVLPLLQLLALLGLLLPLARGRMPADMAQACRLLGWLRPWSMVPVFVLGTVVSLVKL
                     ASAADVHMGMGLPAFGLLTILLTMLGRLSPKVLWRYAETAGLAEVREPEYKPGHTLVG
                     CHACGQVQDLADTESGDETPHCVRCHAVLHRRKPQYLARTWALLLAAAILYIPANVLP
                     VMRIASVVGDSDHTILGGVVELWDGGSWDIALIVFVASVVVPLTKLLSLALLALRAQW
                     GDVTGLRARTRLYELVEFIGQWSMLDVFVVILLSALADFPGLMRISADAGAAAFGMVV
                     VLTMLAAMSFDPRRAWDEEDNALTRIKELH"
     gene            5362..7020
                     /locus_tag="VGC69_04465"
     CDS             5362..7020
                     /locus_tag="VGC69_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0294575.1"
                     /translation="MADQTPPPEKPAAPAPVIVRRRKSRISWIWLVPLVAALAGLSLV
                     VRTWMQAGPEITISFSTAEGLVVGQTQVRYKNVVIGVVNHIHFNHDRSKVLVDASLNK
                     ESADIAREGTRFWVVRPRLGFTGVSGLGTLLSGAYIGVDIPQDSDLRGKPEKLNFQGL
                     ETPPAVTHDLAGRHFTLKAADLGSLDIGSPVYFRRISVGRVVGYKLDEKGDSVDVDVF
                     VDAPNDRYVTQHARFWNASGVDVSVNADGLKMRTESLVSLAIGGLAFETPDDLHDDPA
                     ATEGAQFKLYDSETEARSVPRGLAYAVRMRFDQSVRGLAVGAPVDFQGITLGTVSAIN
                     IDFDPQKKRFYALVDATLYPERLGNVLSKVESRLKPDERAPDLLLSEMVKYGLRAQLR
                     PANLLTGQLFVVLDSFPNTPPVDFVLTKPPMIPTVPGQFDQLQEQVGSILSKISKVPF
                     DQIGTALRDTLTNTSSLMAQLDKQVTPEARGVLRQARQSLESVDKLLAPDAGLATGTQ
                     RTMQELDRAARSLRALADYLQANPESLLRGRGSDPIPGAGPVKN"
     gene            7031..7750
                     /locus_tag="VGC69_04470"
     CDS             7031..7750
                     /locus_tag="VGC69_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEY0294576.1"
                     /translation="MSNRIMIKAETTRGMARRLPPSGRPAHRLFALASAAALAALAGC
                     AGSPQVRYYTLTTPVSVETAQAPAAVADYAIEVMPVSVPAQVDQPQLMLRSGSGELTA
                     QYSDRWSAPLPDELRNALSDTLTRQLGAPDVHQLAPAASLPVWRVQVDVQRFDASTTG
                     PAVIDATWRVRPVQGEGGAMLCRNRVEVPVAPGPGLDAVIVAEQQAVALLGRTIASAV
                     QSQGRQAVAASDQVQAPRCTR"
     gene            7952..8710
                     /locus_tag="VGC69_04475"
     CDS             7952..8710
                     /locus_tag="VGC69_04475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294577.1"
                     /translation="MYLFMLSPLIVVVWLSFFKDAILFFPPSGYTLQWYAKAWANDAF
                     TKGFVFSLQVALLAAVGGVVVGVLAAVGISRYRFKGAGAANTLLLSPLLIPGIVAGVA
                     IYLFYLGAENFLDMDIVGTYGGLVIAHVCLTIPWTVRLVSASMAGLDPSIEEAARNLG
                     ASSRVALLRITLPMMRPAIVAAALFSFVVSFENLGLSLSLVGPGHTTLPIAIMQYLEF
                     NLDPTIAVVATVQIVLLGIIMLVTDRFVKLSQVV"
     gene            8715..9761
                     /locus_tag="VGC69_04480"
     CDS             8715..9761
                     /locus_tag="VGC69_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294578.1"
                     /translation="MAKVSLDRLKKQFGNAVAVSDFSLDIADGELVAFLGPSGCGKTT
                     TLRMIAGFIEPTAGAIRIGDTDVTPLPVHKRDTGMVFQRYALFPHMTVAQNVSFGLEM
                     HKVPMAERAARIRVALDMVRMSALSDRYPRQLSGGQQQRVAIARALAIEPKVFLLDEP
                     LSNLDAKLRGEVREEIRALQQRLGLTTIFVTHDQEEALAIADRMAIMHDGKVQQVGAP
                     QALYERPANLFVADFLGKMNFFKGALDGEAAFVTEHGTRVAIAAPVPRATHAGVRPER
                     LRLAARPAEGNALPGTVESAVYLGAQIEVRVKLDSGASVMAQAANGTLGIAAQPGERV
                     HVCFQPADCVLFAE"
     gene            9773..10654
                     /locus_tag="VGC69_04485"
     CDS             9773..10654
                     /locus_tag="VGC69_04485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294579.1"
                     /translation="MPAASASARSPRLLGASMAFPASLVVLVIIIVPIVLLFRYSFNH
                     FDKVEMMKQAFTFENYARFFSDPYYLGVFYSTLWVAALCTVLALVLGFPVAYFLAKTQ
                     SRFKSLFIILLVFALMVGNVVRSAGWMILLGRAGVLNAILTGLGLVKDPVQFMYTPAA
                     VVIGSTAVVMPYFILTLQSVLEGIDFSVEEAAQNLGANFLTAFWRVVLPIAAPGVAAG
                     TVLVFILCMNAYATPVLLGGTGITMMAPALYDQITRSSNWPFGAAMALILVCATLLMA
                     LLSNWLIHRRYVKTMAS"
     gene            10698..>11162
                     /locus_tag="VGC69_04490"
     CDS             10698..>11162
                     /locus_tag="VGC69_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0294580.1"
                     /translation="MTAVLFKNAALLDPLQPELREGFDVLVEDGLVKEVSDRPLQMAG
                     AQAIDLAGKTLMPGLIDLHVHVLATQLNLSSQGVLPDALVMLRAVPIMAAMLRRGFTT
                     VRDAGGAGWGLKCAIEEGTVDGPRLFISGRAISQTGGHGDPRPRSDHLRPMSF"
BASE COUNT         1799 a         3748 c         3676 g         1939 t
ORIGIN      
        1 gggcagcccc gcaggggccg agggcgcgtc gtcttgcgcc cgagccgccg cgatgcagcc
       61 cgcacccagc cccggccccc gacggacggg tttagcagtg caagtctcgg ggtctcaaat
      121 aacgacgcat acggcgccaa aagatcaagg cagcccgtcg atcctgtcgc ccgccttgat
      181 ccccttgccg acaaaccagc cctgggccat ttccagcgca tagcgtaccg gcttgcgcga
      241 gcagtggctg tcgtcggttt cggcggccat gttctcgagg ttgacgatgg tgccgtcgtc
      301 ggccatgaag gcgatggaca ggggccgtgg cgtgttgtgc atccagaagc actgctggtc
      361 gggcacctcg aagacgaaca gcatgccgtc gttgccgggc agcgccgggc ggaacatcag
      421 cccgcgctcg cgctcgctgt tggtgtcggc gacctcggcg tggatgacgt ggatgccggc
      481 ggccagctgg atgacgggca actggggctg cggcccttgc gcttgctggc cttgcgccgc
      541 ggccagcagg ggagccgcca gcagcacggc ggccagcagc ggttgaaaca gggggcggga
      601 tgggaaggag cggcgcttga tgagggacgg catgtgcggc gggcgtggga aaataccaat
      661 gaaaaaggcg aggggtggac cctcgccttg catgatacct ttgaagatca ggccgcagtg
      721 atgttcagtg cctgcttgcc cttggggcct tggatgatct cgaacgaaac cttctggcct
      781 tccttcaggg ttttgaaacc gttcatctgg atggacgaga agtgggcgaa caggtcctcg
      841 ccgccatcat cgggcgtgat gaagccgaag cctttcgcgt cgttgaacca tttgacggtg
      901 cccgtggctt taggattgtt ccccgggaca gcggatgcgg tcgtatcaga catgcggact
      961 gcctcttgta tcgtatgttg actaaagggc tcggcaagtt gccgcgtccc ctggagtccg
     1021 gtgcggcaaa ccaagcaagt cgcctgtaag cctcccgaat ggaaccgata atgctttgat
     1081 tttctttcaa tagtcaagta tggaaaccgc gcatgtccgc atgtaatttt cagtaattgc
     1141 catcatgtcg cccaggtttg actagaataa gcaggcttat gagttcgaca ttggataccc
     1201 agaacgatca ggtcgtggaa aaggcgccag ccaagaccgc gccgccaccc atgtaccagg
     1261 tggtcctgct caacgacgac tacactccca tggagtttgt ggtcaaagtg ctgcagaaat
     1321 tcttcggcaa gagccacgaa gaggccaccc gcatcatgct tcaggtgcac cacgaaggcc
     1381 gcggcatctg cggggtctac ccgcgcgacg tcgccgccac gcgcattgcg caggtgtcgc
     1441 aatacgcccg cgcgcgccag caccccctgc aatgcgtgat ggaacccgta taaaagcaat
     1501 agaaaaaccg ggaaaccccc atagtcttgc ccatgcagga cgtccgcgtg gggtatgctg
     1561 gctgtgcctg tccagctcgt ctcgcgccgt tgtttttccg gcgaatcttg attgctggcg
     1621 gggcatcccc atataggctg ggtatgcacg tgtgcttatt cgtggcggcc cgcatgggaa
     1681 atcactggcg ctgagcgccg ataggcatag aataaatagc gcgtacgtgg ccgctttggc
     1741 atgtattcgg taagacccgt ggcaagaccc ggaggaagcg tgatttctca agaacttgaa
     1801 gtcagcctgc atatggcgtt tgtcgaggcg cgttccgccc ggcatgaatt tattacggtg
     1861 gagcacctgc tgctgtcact gctggacaat gcgtcggccg tcgaagtgct tcgagcctgc
     1921 gccgccaacc tggatgacct gcgccgcaac ctgcgccaat tcgtcaccga gaacacgcct
     1981 gtcattccca gcgggtccga ggtcgatacc cagcccacgc tgggtttcca gcgtgtgatc
     2041 cagcgcgcca tcatgcacgt ctcggccggc ggcaccggca agaagccggt caccggggcc
     2101 aacgtgctgg tcgccatctt cggcgagaag gactcccacg ccgtgtacta cctgcaacag
     2161 cagggcgtca cgcgcctgga cgtggtcaat ttcctgtcgc acggcatcac caagcagccc
     2221 caggaagagc ccgccgcgcc ccagaaagag cagcaggcca gcgcggaaga gcagggcggc
     2281 gagggccgcc agtcgccgct ggaccagttc gccaccgatc tcaacgccgc cgccctggcc
     2341 ggccgcatcg atccgctcat cggccgcgag cacgaggtcg agcgcgtcat ccaggtgctg
     2401 tgccgccgcc gcaagaacaa cccgctgctg gtgggcgagg ccggcgtggg caagaccgcc
     2461 atcgccgagg gcctggcctg gcgcattacg cgcggcgagg tgcccgaggt gctgcagacc
     2521 tcccaggtct tcgcgctcga catgggcgcg ctgctggccg gcaccaagta ccgcggcgat
     2581 ttcgagcagc gcttgaaggg tgtgctcaag caggtgaagg ccaaccccga cgccatcctc
     2641 ttcatcgacg agatccacac gctgatcggc gccggttccg cctcgggcgg cacgctcgac
     2701 gcctccaacc tgctcaagcc ggccctgtcc tcgggccagc tcaagtgcat cggcgccacc
     2761 acctacaacg aataccgcgg cgtgttcgag aaagaccacg ccctgtcgcg ccgtttccag
     2821 aaaatcgacg tgcccgagcc cagcgtcgag cagaccgtgc agatcctgcg cggcctgaag
     2881 agccgcttcg aagagcacca caacgtgcgc tattcggcgg cggcgctgtc ggccgcggcc
     2941 gagctggcgg cccgctacat caacgaccgc cacctgcccg acaaggccat cgacgtcatc
     3001 gacgaggccg gcgccgcgca gcgcctgctg gccgcgtcca agcgcaagaa ggtcatcggc
     3061 aaggccgaga tcgaggccat cgtctccaag atcgcccgca ttccgccgca gtccgtctcc
     3121 aacgacgacc gcagcaagct ggccaccctc gaccgcgacc tgaaaaccgt cgtgttcggc
     3181 caggaccagg ccatcgaagc cctgtcggcc gccatcaaga tggcgcgctc cggcctgggc
     3241 aagcccgaca agcccatcgg cgccttcctg ttctccggcc ccaccggcgt gggcaagacc
     3301 gaagtggcca agcagctcgc cttcaccctg ggcgtagagc tgctgcgctt cgacatgtcc
     3361 gagtacatgg agcgccacgc cgtgtcgcgc ctcatcggcg cgccgccggg atacgtcggc
     3421 ttcgaccagg gcggcctgct taccgaggcc atcaccaagc agccgcactg cgtgctgctg
     3481 ctcgacgaaa tcgagaaggc ccaccccgac atcttcaaca tcctgctgca ggtcatggac
     3541 cacggcacgc tgaccgacaa caacgggcgc aaggccgact tccgcaacgt catcatcatc
     3601 atgacgacca acgcgggcgc cgagacgctc aaccgtccca ccatcggctt cgccaacagc
     3661 cgcgaggccg gcgacgagat ggtcgagatc aagcgcatgt tcacgcccga gttccgcaac
     3721 cggctcgacg cgatcatccc cttcgggtcg ctcacgcgcg acatcatcct gcgcgtggtc
     3781 gacaagttcc tcatgcagct cgaagaccag ttgcaccagc gccgcgtcga ggccgtcttc
     3841 accgaacgcc tgcgcgcgta cctggccaag acgggtttcg atccggtcat gggggcccgt
     3901 cccatgcagc ggctcatcca ggacaccatc cgccgcgccc tggccgacga gctgctgttc
     3961 ggcaagctgg tcgatggcgg taccgtcacc gtggacgtcg acgaagcgga caaggtcgtc
     4021 ctcagtttcg aagacggcaa gcccactggc agttcggctc ctcctgagga agtcgcgctg
     4081 gccgactaag ctttggacga ccagccgctg atcgcctgtg agcactgcgc cagcatctat
     4141 cgccggcgcg tcctgaggca tggggaactg gcggcctgca cgcggtgcgg cacggtgctg
     4201 tggcgctaca gccggctgac cttgaacgaa tggctcgcca tcgccttggc gggccttttg
     4261 cttttctgca tcgccaacgc ctatccggtg gcccggatcg aggtctacgg cgccgaacgc
     4321 ggcgccacgc tgctcgatgc cgtgttcatc acctggcagc agcaccattg gctggtgtcg
     4381 atcatgacgg gcctggccgg cttcgtgctg ccgctactgc aattgctcgc gctgctgggg
     4441 ctgctgctgc ccctggcccg cggccggatg ccggccgaca tggcgcaggc ctgccgcctg
     4501 ctgggctggc tgcggccctg gagcatggtg cccgttttcg tgctgggcac cgtcgtgtcg
     4561 ctggtcaagc tggccagcgc ggccgatgtg cacatgggca tgggcttgcc ggccttcggc
     4621 ctgctgacca tcctgctcac catgctgggc cggctgtcgc ccaaagtatt gtggcgctat
     4681 gccgagaccg ccggcctggc cgaggtgcgc gagcccgagt acaagccagg gcatacactg
     4741 gtgggctgcc atgcctgcgg gcaggtgcag gatctggccg atacggagtc cggcgacgag
     4801 acgccgcatt gcgtgcgctg ccatgcggtg ctgcaccggc gcaaaccgca gtacctggcg
     4861 cgcacttggg cgctgctgct ggccgcggcc attctctata taccggccaa tgtcctgccg
     4921 gtcatgcgca tcgcttccgt cgtgggcgac agcgaccaca ccatcctggg cggcgtggtc
     4981 gagttgtggg acggcggttc gtgggatatt gcgctgatcg tgttcgtggc cagcgtggtc
     5041 gtgccgctga ccaagctgtt gtccctggcg ctgctggccc tgcgcgcgca gtggggcgac
     5101 gtcacgggcc tgcgggcgcg cacccggctc tacgagctgg tcgagttcat cggccaatgg
     5161 tccatgctcg atgtattcgt ggtgatcctg ctctcggcgc tggcggattt ccccggcctg
     5221 atgcgcatca gcgccgatgc cggcgcggcc gccttcggga tggtggtggt gctgaccatg
     5281 ctggccgcca tgagtttcga cccgcgccgc gcctgggacg aggaagacaa cgcgctgacg
     5341 cgcatcaagg agttgcattg aatggctgat cagacgccgc ccccggaaaa gccggccgcg
     5401 ccggcccccg tcatcgtgcg caggcgcaag agccgcatct cctggatctg gctggtgccg
     5461 ctggtggcgg ccctggccgg cctgtcgctg gtggtgcgca cctggatgca ggccgggccc
     5521 gagatcacca tctcgttcag cacggccgag gggctggtcg tcggccagac ccaggtgcgc
     5581 tacaagaatg tggtgatcgg cgtggtcaac catatccatt tcaaccacga ccgcagcaag
     5641 gtgctggtcg acgcctcgct caacaaggag tcggccgaca tcgcgcgcga aggcacgcgc
     5701 ttctgggtgg tgcggccgcg cctggggttc accggcgtgt cgggcctggg cacgctgctg
     5761 tccggcgcct atatcggcgt ggacatcccg caagacagcg acctgcgcgg caagcccgaa
     5821 aagctcaatt tccagggcct ggagacgccg ccggccgtta cccatgacct ggcgggcagg
     5881 catttcacgc tcaaggcggc cgacctgggc tcgctggaca tcggctcgcc ggtgtatttc
     5941 cgccgcatca gcgtgggccg ggtcgtgggc tacaagctcg acgagaaagg cgattcggtc
     6001 gacgtggacg tattcgtcga cgcgcccaat gaccgctatg tcacccagca tgcgcggttc
     6061 tggaatgcca gcggggtgga tgtgtcggtc aatgccgacg gcttgaagat gcgcaccgag
     6121 tcgctggtgt cgctggccat cggcggcctg gccttcgaga cgccggacga cctgcacgat
     6181 gatccggcgg ccaccgaggg cgcgcagttc aagctgtacg acagcgagac cgaggcgcgt
     6241 tccgtgccgc gcggcctggc ctatgcggtg cgcatgcgct tcgaccagtc ggtgcgcggc
     6301 ctggccgtgg gcgcgccggt cgatttccag ggcattacgc tgggtacggt gtcggccatc
     6361 aacatcgatt tcgatccgca gaaaaagcgg ttctatgcgc tggtcgacgc caccttgtat
     6421 ccggagcgcc tgggcaatgt gctcagcaag gtcgagtccc gtctcaagcc cgacgagcgc
     6481 gcgcccgacc tgctgctgag cgagatggtg aagtacggcc tgcgcgccca gctgcggccg
     6541 gccaatctgc tgacggggca gctcttcgtc gtgctggatt ccttccccaa caccccgccc
     6601 gtcgatttcg tcctgaccaa gccgcccatg attccgacgg tgccgggcca gttcgaccag
     6661 ttgcaggaac aggtgggcag tatcctgtcc aagatctcga aggtgccttt cgaccagatc
     6721 ggcaccgcgc tgcgcgacac gctcaccaac acctcgagcc tgatggcgca gctcgacaag
     6781 caagtcacgc cggaggcgcg gggcgtgctc aggcaggcgc ggcagtcgct ggagtcggtc
     6841 gacaagctgc tggcgcccga tgccgggctg gcgaccggca cgcagcgcac catgcaggaa
     6901 ctcgaccggg ccgcgcgttc gctgcgcgcc ctggcggact atctgcaggc caatcccgag
     6961 tccctgctgc gcggccgcgg cagcgatccc atccccggtg cggggcccgt aaagaactga
     7021 tgaacgagac atgtcgaatc ggatcatgat caaggcagaa acaacccggg gcatggcgcg
     7081 gcggctgccg ccttccgggc ggccggcgca tcgcctgttc gcgctggcgt cggccgcggc
     7141 cctggcggcg ctggccggct gcgccggctc gccgcaggtg cgctattaca ccttgacgac
     7201 gcctgtgtcc gtcgaaacgg cgcaagcgcc ggccgcggtg gccgattacg ccatcgaggt
     7261 catgccggtg tccgtgccgg cccaggtcga ccagccgcag ctcatgctgc gcagcggttc
     7321 gggcgaactt accgcccagt attcggaccg ctggagcgcg cccttgcccg acgaattgcg
     7381 caatgccttg tccgacacgc tgacccgcca gctgggcgcg cccgatgtgc accagctggc
     7441 gccggcggcc agcctgccgg tctggcgggt gcaggtggac gtccagcgtt ttgacgcgtc
     7501 gacgaccggg ccggcggtga tcgacgccac ctggcgggtg cgccccgtgc aaggcgaggg
     7561 cggggccatg ctgtgccgca accgggtcga agtgccggtg gccccgggcc ccggcctgga
     7621 cgcggtgatc gtggccgagc agcaggccgt cgccctgctg gggcgcacca tcgcctcggc
     7681 cgtgcagtcg caaggccgcc aggccgtggc cgcttcggac caggtgcagg cgccgcgctg
     7741 cacgcgctag ttcctcaagg gccggattta gtggttaacc agtattttta aaaaggtgca
     7801 acctccttag gattgcggta ctaccgattg cagtactaag gaaatcgtct tgtccatcac
     7861 tgccacgccc gatgggttgc gcgtgccggc gcccgcgcgg caagggcgct tcccctgggc
     7921 ccgctgcctg caccgcgcct tcgtggcgct gatgtaccta ttcatgctca gcccgctgat
     7981 cgtcgtggtc tggctgagct tcttcaagga cgccatcctc tttttcccgc cctccggcta
     8041 cacgctgcag tggtatgcca aggcctgggc caatgacgcc ttcaccaagg gcttcgtctt
     8101 cagcctgcaa gtggcgttgc tggcggcggt cggcggcgtg gtcgtcggcg tgctggccgc
     8161 ggtgggcatc agccgctacc gcttcaaggg cgcgggcgcg gccaacacgc tcctgctctc
     8221 gccgctgctc atccccggca tcgtcgccgg cgtggcgatc tacctgttct acctgggcgc
     8281 ggaaaatttc ctcgacatgg acatcgtcgg cacctacggc gggctggtca tcgcccacgt
     8341 gtgcctgacc atcccctgga cggtgcgcct ggtgtcggcc agcatggccg ggctggaccc
     8401 ctccatcgag gaggccgcgc gcaatctcgg cgccagcagc cgggtcgcgc tgctgcgcat
     8461 cacgctgccc atgatgcggc cggccatcgt ggcggcggcc ctgttcagct tcgtggtctc
     8521 gttcgagaac ctgggactgt cgctgtcgct ggtggggccg gggcacacca ccctgcccat
     8581 cgccatcatg caatacctgg aattcaatct ggaccccacc atcgcggtgg tggcgacggt
     8641 ccagatcgtg ctgctgggca tcatcatgct ggtgaccgac cgcttcgtca aactcagtca
     8701 agtggtatag aaaagtggcc aaagtctctc tggataggct caagaagcaa ttcggcaacg
     8761 cggttgccgt cagtgatttc tcgctcgaca tcgccgatgg cgaactggtg gccttcctgg
     8821 gcccgagcgg ctgcggcaag accaccacgc tgcggatgat cgccgggttc atcgaaccga
     8881 cggcgggcgc catccgcatc ggcgacaccg acgtcacgcc gctgccggtg cacaagcgcg
     8941 ataccgggat ggtcttccag cgctatgccc tgtttcccca catgacggtc gcgcagaacg
     9001 tctcgttcgg cctggagatg cacaaggtgc ccatggccga acgggcggcg cgcatccgcg
     9061 tcgccctcga catggtgcgc atgtcggcgc tgagcgaccg ctatccgcgg cagttgtcgg
     9121 gcggccagca gcagcgcgtg gccatcgccc gcgcgctggc catagagccc aaggtcttcc
     9181 tgctggacga gccgctgtcc aacctggacg ccaagctgcg cggggaggtg cgcgaggaga
     9241 tccgggcgct gcagcagcgc ctgggcctga ccaccatctt cgtgacccac gaccaggaag
     9301 aagccctcgc catcgccgac cgcatggcca tcatgcatga cggcaaggtg cagcaggtgg
     9361 gcgcgccgca ggcgctgtac gagcggcccg ccaacctgtt cgtggccgac ttcctgggca
     9421 agatgaattt cttcaagggc gcgctcgacg gcgaagcggc cttcgtgacg gagcacggca
     9481 cgcgcgtggc gatcgctgcg ccggttccgc gcgccacgca cgccggcgtg cggcccgagc
     9541 gcctgcggct ggcggcgcgg ccggccgagg gcaacgccct gccgggcacg gtcgagtcgg
     9601 cggtgtacct gggcgcgcag atcgaggtcc gcgtcaagct cgacagcggc gcgtcggtga
     9661 tggcccaggc ggccaacggc accctcggca tcgcggcgca gccgggcgag cgggtccacg
     9721 tgtgcttcca gcccgccgac tgcgtgctgt tcgccgaatg aggagggcag gcatgccagc
     9781 cgcatcggcc tccgcgcgat cgccccgcct gctgggcgcc agcatggcct ttcccgcctc
     9841 gctggtggtg ctcgtcatca tcatcgtgcc catcgtgctg ctgtttcgct acagcttcaa
     9901 ccatttcgac aaggtcgaga tgatgaagca ggccttcacg ttcgagaact acgcgcgctt
     9961 tttctcggac ccgtactacc tcggcgtgtt ctattccacg ctgtgggtgg ccgcgctgtg
    10021 cacggtgctg gcgctggtgc tgggctttcc cgtggcctat ttcctggcca agacccagag
    10081 ccgcttcaag agcctgttca tcatcctgct ggtgttcgcc ctgatggtgg gcaacgtggt
    10141 gcgttcggcc gggtggatga tcctcctggg ccgggccggc gtactcaacg ccatcctgac
    10201 cggactgggg ctggtgaagg atcccgtgca gttcatgtac acgcccgcgg ccgtggtgat
    10261 cggctccacg gccgtggtca tgccctattt catcctgacc ttgcagagcg tgctggaggg
    10321 catcgacttc tcggtggagg aggcggccca gaacctgggc gcgaacttcc tgacggcctt
    10381 ctggcgcgtg gtgctgccga tcgccgcgcc cggcgtggcg gccggcaccg tgctggtctt
    10441 catcctgtgc atgaatgcct atgccacgcc ggtcctcctg ggcggcacgg gcatcaccat
    10501 gatggccccc gcgctctacg accagatcac gcgcagctcg aactggccgt tcggtgcggc
    10561 catggcgctc atcctggtgt gcgcgaccct gctgatggcc ctgctgtcga actggctgat
    10621 ccatcgccgg tacgtgaaga ccatggcctc ctgatccact catcggcggc ccgacgcgcc
    10681 gcctgggagc aatgcgaatg actgctgtac tgttcaagaa cgccgccctg ctcgaccccc
    10741 tgcagcccga gctgcgggag ggcttcgatg tcctggtcga ggacgggctg gtcaaggaag
    10801 tctccgaccg gcccctgcag atggccggcg cgcaggccat cgacctggcc ggcaagacgc
    10861 tgatgcccgg cctgatcgac ctgcacgtcc acgtgctggc cacccagctc aacctgagct
    10921 cgcagggcgt gctgcccgac gcgctggtca tgctgcgcgc cgtgcccatc atggcggcca
    10981 tgctgcgccg cggctttacc acggtgcgcg acgcgggcgg ggccggctgg gggctgaagt
    11041 gcgccatcga ggaaggcacg gtcgacggtc cgcgcctgtt catctcgggg cgcgccatca
    11101 gccagacggg cggccacggc gacccgcgtc cgcgctcgga ccacctgcgc cccatgagct
    11161 tc
//
LOCUS       DASWPO010000075        12441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1625502, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000075 DASWPO010000000
VERSION     DASWPO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625502"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /gene="hfq"
                     /locus_tag="VGC69_04495"
     CDS             complement(<1..232)
                     /gene="hfq"
                     /locus_tag="VGC69_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY0294581.1"
                     /translation="MSNKGQTLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPARAVNFQVDVPA"
     gene            complement(387..1469)
                     /gene="hisC"
                     /locus_tag="VGC69_04500"
     CDS             complement(387..1469)
                     /gene="hisC"
                     /locus_tag="VGC69_04500"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0294582.1"
                     /translation="MSKYWSPVVSQLDPYSPGEQPKHRNVVKLNTNESPYGPSPKALQ
                     AMRDACNDNLRLYPSFTSDRVRQAVSANLGVPVEHVFVGNGSDEVLAHAFNAFFQQPR
                     PLLFPDISYSFYPTYCGLYGIDYQPVPVTADFRIEPADFLPDAARAQPGPAGGIILPN
                     PNAPTSLALTLAEIERIVAGNPQCVVVIDEAYVDFGAESAVPLTARYDNLLVIQTLSK
                     SRSLAGLRVGFAIGSRELVEGLQRVKDSFNSYPVDSVAEAGAAAAIEDRAYFDQTRQA
                     VMRSRATLDTALRKLGFETLPSKTNFIFARHPGHDAAELAAGLREHGILVRHFRRPRI
                     EQFLRITVGTDPQCAQLVQALSELLA"
     gene            complement(1480..2841)
                     /gene="der"
                     /locus_tag="VGC69_04505"
     CDS             complement(1480..2841)
                     /gene="der"
                     /locus_tag="VGC69_04505"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0294583.1"
                     /translation="MSFKPVIALVGRPNVGKSTLFNRLTRSRAAIVADYSGLTRDRHY
                     GEGRVGETPFIVIDTGGFEPVAKDGILVEMARQTRQAIAEADVVIFLVDARAGINAHD
                     HEIARLLRKSGQQRVLVAVNKAEGMGSGKAESDFHELGLGEPYPISAAHGDGIADLVE
                     LALEGLVPPPEAEPEVSEGGELGEAIPHRIKLAIVGRPNVGKSTLINTLMGEERVIAF
                     DLPGTTRDAIEIDFDRDGKRYTLIDTAGLRKRGRVFESVEKFSVIKTLQAIEACNVVV
                     LVLDAQTEVSEQDAHIAGFVLETGRAVVVAINKWDGLDVEQREQIEREFQRKLRFLSF
                     AKTHTISALKGQGIKPLLKSVNEAHAAAFAKLSTPRLTRELQAAVEQQQPPRKGIFRP
                     KMRYAHQGGQNPPLVIVHGSALDAIPDSYRRYLETRFRNAFELIGTPLRIEFKSSHNP
                     YVD"
     gene            complement(2860..4014)
                     /gene="bamB"
                     /locus_tag="VGC69_04510"
     CDS             complement(2860..4014)
                     /gene="bamB"
                     /locus_tag="VGC69_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEY0294584.1"
                     /translation="MRLFHNRVWRGALIAGSLMALAGCSLFSHDDDRYKPAELTEYAP
                     GLAVHAVWQVSVGSGSDMGFVPAVVGDSAYAATPNGSVVKVDLASGRVVWRASAQKDL
                     SAGVGSDGTVTAVATDSGDVIAFDDTGKIKWKAKATSDVHIPPVVGDGIVAVRSGDYR
                     IQAFDINTGELLWSVQRPGPPLALRGTSQMLVTERMVVAGMPGGKVMAISANNGVVQW
                     EGSVATPKGVTDLERLSDVVGRPIAAGPLLCAVTYQGRIACFNVGDGGRLAWAKDFSS
                     SVGMALDSNAVYAPDAHDVVNAFALDTGAPLWKQTALSNRSLSAPATVGNAVALGDFE
                     GYVHFLSAADGHLLARLSVGGGAVLSAPQSTPQGVLVQTGGGNLVLIGVN"
     gene            complement(4020..4670)
                     /locus_tag="VGC69_04515"
     CDS             complement(4020..4670)
                     /locus_tag="VGC69_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0294585.1"
                     /translation="MAYYLEEQEKLDAIKAWWSRYGTLALSVATVVVLAAAGWSGWRA
                     YNGYRASQAMGYFEALEDAARGVNGPGGADATKRITAAADTLRTDYAGTAFAARGVLI
                     AAQALADQKDADGARRQLEWLVGRKDQPALQPVARLRLAGLLLDQKQYDQALAQLADP
                     PAAFAALYADRRGDILAAQGKRDDARAAWQSALSTMPAGDPLMQIVQLKLDALSGA"
     gene            complement(4684..5994)
                     /gene="hisS"
                     /locus_tag="VGC69_04520"
     CDS             complement(4684..5994)
                     /gene="hisS"
                     /locus_tag="VGC69_04520"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY0294586.1"
                     /translation="MTQAFQKVTAIRGMNDLLPGDSARWEQFEEIVRDWLHGYGYRNV
                     RTPVLEHTRLFARGIGEVTDIVEKEMYTFTDSLNGDSLTLRPEMTAGIVRASIEHNLL
                     YDRPQRVYSVGPVFRHERPQRGRYRQFHQIDAEALGFAGPDVDAELIVMLARLWKRLG
                     LADVRLELNSLGQPAERAAHRAALIEHLEQYQDILDEDGKRRLYTNPLRVLDTKNPAM
                     QDMADSAPRLFDFLGDESRAHFDGVCQRLADAGIEYRLNPRLVRGLDYYNLTVFEWVT
                     DRLGSQGTVCGGGRYDGLIELLGGKAAPAVGFAIGVERLLDLWEQTLAASIEQPAPVP
                     ECDVYVVHQGDAAQRLAAQAAESLRDAGLAVIVHAGSGSFKSQFKRADVSGARVAVIL
                     GDDEVANKAAGVKFLRGATLEAGGQQQVPLARLAEAVQAALNTK"
     gene            complement(6052..7350)
                     /gene="ispG"
                     /locus_tag="VGC69_04525"
                     /gene_synonym="gcpE"
     CDS             complement(6052..7350)
                     /gene="ispG"
                     /locus_tag="VGC69_04525"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0294587.1"
                     /translation="MPTMQDETSLPCQDLPPAVGPAARRVTRAVDIRWNDRVVTVGGG
                     APVMVQSMTNTDTADAIATAIQVKELALAGSEVVRITVNTPEAAREVPAIREQLDRMG
                     MSVPLVGDFHYNGHKLLTQFPECAQALSKYRINPGNMGGGKRRDDNFAQMIEVACRYE
                     KPVRIGVNWGSLDHELMARMMDDNGRRAQPWEAQAVMRDALVVSAISNAQRAEALGLP
                     GDAIILSCKVSHVQDLIAVYRDLSARCDYPLHLGLTEAGMGSKGIVASTAALAVLLQE
                     GIGDTIRISLTPEPGGDRTREVIVAQEILQTMGLRAFTPLVVACPGCGRTSSTVFQEL
                     ADSIQGYLRRQMPAWRLQYPGVENMNVAVMGCVVNGPGESRHADIGISLPGTGEVPAA
                     PVFVDGERTVTLKGDRIAEEFQVIVEDYVARRYGTAAATH"
     gene            complement(7355..7831)
                     /locus_tag="VGC69_04530"
     CDS             complement(7355..7831)
                     /locus_tag="VGC69_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0294588.1"
                     /translation="MIQQDSTVVDETSQTKSVGSALRDLRIARGLSLEDVSARLKYST
                     RQIQALEEERWDQLPSGVSLRGMIRNYARLVGADMEPIVEALSSHASVSIRPTGRDLG
                     ASAAVGGEDAPHASSVPWGWLLVILVVVIAVVAYAFWQGWLPKELLSLDGLKQRFH"
     gene            complement(7828..9000)
                     /gene="rlmN"
                     /locus_tag="VGC69_04535"
     CDS             complement(7828..9000)
                     /gene="rlmN"
                     /locus_tag="VGC69_04535"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY0294589.1"
                     /translation="MENERINLLGQDAAALSELVGQWGGKPFRARQLQRWVHQRGVDS
                     FDAMSDLARDFRAQLSERCRVQALPVSTEQVSSDGTRKWLFDVGQGNAIETVFIPEDD
                     RGTLCVSSQAGCAVNCRFCSTGHQGFSRNLTTAEIVGQLWWARRVLVADAGSARLPAG
                     DAKSGGAKSEGEQQDPRVISNVVMMGMGEPLLNYDQLVPALRLMLDDNAYGLSRRRVT
                     VSTSGVVPMMDRLAQDCPVALAVSLHAPNDALRDELVPLNRKHPLKSLLEACENYLAY
                     APRDFITFEYCMLDGINDTDQHAKELIQLARQIRCKLNLIPFNPFPASGLKRSPQARV
                     RVFAQRLMDAGIITTVRKTRGDDIAAACGQLAGEVQDRTRLAERKSRTIPITQVSA"
     gene            complement(9049..9498)
                     /gene="ndk"
                     /locus_tag="VGC69_04540"
     CDS             complement(9049..9498)
                     /gene="ndk"
                     /locus_tag="VGC69_04540"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY0294590.1"
                     /translation="MATTTTIQSTAQRTLSIIKPDAVAKNVIGQIIARFETAGLKVIA
                     AQMRQLSRADAERFYAVHSARPFFKDLVDFMVSGPVFVQVLEGEEAILKNRDLMGATD
                     PKKAEKGTIRADFADSIDANAVHGSDAPETAAVEIAFFFPEINVHSR"
     gene            9719..>12441
                     /locus_tag="VGC69_04545"
     CDS             9719..>12441
                     /locus_tag="VGC69_04545"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY0294591.1"
                     /translation="MTQAAESTTPATAATAAATDSPELAKSFEPAEIESRWYAQWEQR
                     GYFQAGRHVKTGTDDKSYCIQFPPPNVTGTLHMGHAFNQTIMDGLVRYHRMSGDDTVF
                     IPGTDHAGIATQIVVERQLDAQKISRHDLGREKFVEKVWQWKEQSGNTITGQVRRLGA
                     SADWPREYFTMDERMSRGVVETFVKLYQQGLIYRGKRLVNWDPKLLTAVSDLEVVSEE
                     VDGFLWHIEYPFVDGPQTIVDKDGSTVTLRGMTIATTRPETMLADGALCVHPDDPRYK
                     HLVGKLVELPLCDRNIPIIADDFVDMDFGTGCLKITGAHDFNDYACSQRHNLPLITIF
                     TLDAHINENGPKRFQGLDRDAARKAVVAALEEDGFLVKVVPHKMMQPKGDRTGVVIEP
                     MLTDQWFVAMSKPAPDGAINPGQSITQVALDAVANGRIAFYPENWTTIYNQWLENIQD
                     WCISRQLWWGHQIPAWYSEDGRIFVAKSEAEAIEQARAAGVAGPLKRDEDVLDTWFSS
                     ALVPFTTLGWPENTLDYQRYLPSSVLVTGFDIIFFWVARMVMMTLHMTGQIPFRHVYV
                     HGLIRDADGQKMSKSKGNTLDPVDLIDGVDLDSLVAKRTFGLMNPKQAGTIEKTTRRQ
                     YPDGIPAFGTHALRFTMAAYATLGRNINFDLKRCEGYRNFCNKLWNATRFVLMNTEGH
                     DLHGKEESPHAAPSGQAELSFADKWIVSLLQGLEADVAKGFADYRFDNIANAIYRFVW
                     DEYCDWYLELAKVQIQGGTPAQQLGTRRTLIRVLEVVLRLAHPIIPFITEELWQKVSV
                     VAGKRRADEAGSVSVQPYPVANPAAIDAEAEAAVAELKSQIEAVRALRGEMSLSPAQR
                     VPLFAEGDAQVLGRNAPYLAALAKLSQVDIVAALPDAGAPVQVVGKARLML"
BASE COUNT         2115 a         4073 c         4190 g         2063 t
ORIGIN      
        1 cagcgggaac gtccacttgg aaattgacgg ctcgcgcggg aaccaccgtg gagatggcgt
       61 gcttgtagac catttgcgta accgtgttgc gcagcaggac cacgtattga tcaaacgatt
      121 cgatttgccc ttgaagcttg atgccgttca ctaagtaaat ggacacgggc acatgttctt
      181 tgcgcagcgt gttcaggaac ggatcttgca gagtttgccc tttattgctc attggccagg
      241 ctccatttgt tattgagtta cggcatctaa aaaagaaaag aatgccggaa acgcctactc
      301 tacagggttt tcccggcccg tgccacttgg gatcccaatc aaactacgac cagatttttc
      361 agatagttcc aatgccctgg gaagtcttac gccagcagtt cggaaagggc ctgaaccagt
      421 tgggcgcact gcggatcggt gccgacggtg atgcgcagga actgctcgat gcggggccgg
      481 cggaagtggc gcaccaggat gccgtgctcg cgcaggccgg ccgccagctc ggccgcatcg
      541 tggcccggat ggcgggcaaa aatgaagttg gtcttcgacg gcagggtctc gaaacccagc
      601 ttgcgcagcg cggtgtccag agtggcgcgg ctgcgcatga cggcttggcg ggtctggtcg
      661 aaataggccc tgtcttcgat ggcggccgcg gcgccagcct cggccacgct atccaccggg
      721 taggaattga agctgtcctt gacccgctgc aggccctcga cgagctcgcg gctgccgatg
      781 gcgaaaccca cccgcaagcc ggccagggag cgcgatttgg acagcgtctg gatgaccagc
      841 agattgtcgt acctggcggt cagcggcacg gccgattcgg cgccgaagtc cacataggcc
      901 tcgtcgatga cgaccacgca ttgcggattg ccggcgacga tgcgctcgat ctcggccagc
      961 gtcagggcca ggctggtggg cgcattgggg ttgggcagga tgatgccgcc cgccggaccg
     1021 ggctgggcgc gggcggcatc gggcaggaaa tcggccggct cgatgcggaa gtcggccgtg
     1081 accggcacgg gctgatagtc gatgccgtac aggccgcagt aggtcgggta gaagctgtac
     1141 gaaatatccg gaaacagcag gggccggggt tgctggaaga aggcgttgaa cgcatgcgcc
     1201 agcacttcgt cggagccgtt gccgacgaaa acatgctcga ccggcacgcc caggttggcc
     1261 gagacggcct ggcgcacacg gtcggacgtg aacgacgggt acaggcgcag gttgtcgttg
     1321 caggcgtcac gcatggcctg cagcgccttg ggcgacgggc cgtaggggct ttcgttggtg
     1381 ttgagcttga cgacgttccg gtgcttgggc tgctcgcccg gagaataggg gtctagctgg
     1441 ctgacgaccg gactccagta cttgctcatt cttgtcttct tagtctacgt aggggttgtg
     1501 cgaggacttg aactcgatgc gcaacggcgt gcctatcagc tcgaaggcat tgcgaaaacg
     1561 cgtttcgagg tagcggcgat aggaatcggg aatggcgtcc agggcgctgc cgtggacgat
     1621 gaccagcggc ggattctgtc cgccctggtg cgcatagcgc atcttgggcc ggaagatgcc
     1681 cttgcgcggg ggttgctgct gctcgaccgc ggcctgcagc tcgcgcgtga gcctgggcgt
     1741 ggacagcttg gcgaaggccg ccgcgtgcgc ttcgttgacc gacttcagca gcggcttgat
     1801 gccctgccct ttcaaggccg agatggtgtg cgtcttggca aacgacaaga agcgcagctt
     1861 gcgctggaac tcgcgctcga tctgctcgcg ctgttcgacg tccaggccgt cccatttatt
     1921 gatggccacc accaccgcgc gccccgtctc gagcacgaat ccggcgatgt gggcatcctg
     1981 ctcggacacc tcggtctggg catcgagcac cagcaccacc acattgcagg cctcgatggc
     2041 ctgcaaggtc ttgatgaccg agaacttctc gacggattcg aatacccggc cgcgcttgcg
     2101 caggccggcg gtgtcgatca gggtgtaacg cttgccgtcg cgatcgaaat cgatctcgat
     2161 ggcgtcgcgc gtggtgcccg gcaggtcgaa ggcgatgacg cgctcttcgc ccatgagcgt
     2221 attgatgagc gtggacttgc ccacattggg ccggcccacg atggccagct tgatgcggtg
     2281 cgggatcgcc tcgcccagct cgccgccctc ggaaacctcg ggttcggcct cgggcggcgg
     2341 gaccagccct tcgagggcca gctcgaccag atcggcgatg ccgtcgccgt gcgcggccga
     2401 gatgggatac ggttcgccca ggcccagttc gtggaaatcg gactcggcct tgcccgagcc
     2461 catgccttcc gccttgttca cggccaccag cacgcgctgc tggccggact tgcgcaacag
     2521 gcgggcgatt tcgtggtcgt gggcattgat gccggcacgg gcatcgacca ggaagatcac
     2581 gacgtcggcc tcggcgatgg cctggcgcgt ctggcgcgcc atctcgacca ggatgccgtc
     2641 cttggccacc ggctcgaaac cgccggtatc gatgacgatg aaaggcgtct cgcccacccg
     2701 gccctcaccg taatgacgat cgcgcgtcag gccggaatag tcggccacga tcgccgcgcg
     2761 cgaacgcgtc aagcggttga agagggtcga cttgcccacg ttggggcggc cgaccagggc
     2821 gatgacaggt ttgaacgaca cggtattccg atgaaacgat cagttgacgc cgatgaggac
     2881 gagattgccg ccgcccgtct gcaccagcac gccttgcggc gtggattgcg gcgcggacag
     2941 cacggcgccg ccgcccaccg acaggcgggc cagcaggtgg ccgtcggccg ccgacaggaa
     3001 atgcacgtag ccctcgaaat cgcccagcgc cacggcattg cccacggtgg cgggcgcgct
     3061 caggctgcgg ttgctcaggg cggtttgctt ccagagcgga gcgccggtat ccagcgcaaa
     3121 ggcattgacc acgtcatggg cgtcgggcgc gtagaccgcg ttggagtcca gcgccatgcc
     3181 gacggaactg gagaagtcct tggcccaggc caggcggccg ccgtcgccca cgttgaaaca
     3241 ggcaatgcgg ccctggtagg tcacggcgca cagcaggggg ccggccgcga tcgggcggcc
     3301 caccacgtcg gacaggcgct cgaggtcggt cacgcccttg ggcgtggcca cggagccttc
     3361 ccattgaacg acgccgttat tggcggagat ggccatcacc ttgccgcccg gcatgccggc
     3421 gacgaccatg cgttcggtca ccagcatttg cgaagtgccg cgcagcgcca gcggcgggcc
     3481 gggacgctgc acgctccaga ggagttcgcc cgtgttgatg tcgaaggcct ggatgcggta
     3541 gtcgccgctg cgcaccgcga cgatgccgtc ccccaccacc ggcgggatat gcacgtcgct
     3601 ggtggccttg gccttccact tgatcttgcc cgtgtcgtcg aaggcgatca cgtcgcccga
     3661 atccgtcgcc accgccgtga cggtgccgtc gctgccgacg ccggccgaca ggtccttctg
     3721 ggccgaagcg cgccacacca cgcggccgct ggccaggtcc accttgacca ccgagccgtt
     3781 gggcgtcgcc gcataggcgg agtcgcccac caccgcaggc acgaagccca tgtccgaacc
     3841 gctgcccacc gagacctgcc agacggcgtg cacggccagg ccgggcgcgt actcggtcaa
     3901 ttccgcaggc ttgtagcggt cgtcgtcatg cgaaaacagc gaacagccgg ccaaagccat
     3961 caggctaccg gcaatcaggg caccgcgcca aacgcggtta tggaaaaggc gcatcactat
     4021 caggctccgc tcaaagcatc gagtttcaat tgcacgatct gcatcagggg atcgccggcc
     4081 ggcatggtcg acaacgcact ctgccaggcc gcgcgcgcat cgtcacgctt gccctgcgcc
     4141 gccaggatgt ccccgcggcg atcggcatac agcgccgcga aagcagccgg cggatcagcc
     4201 agttgcgcca gggcctggtc gtactgcttc tggtcgagca gcaggccggc caggcgcagg
     4261 cgggctaccg gctgcagcgc gggctggtcc ttgcggccga ccagccattc gagctggcgg
     4321 cgcgcgccat cggcgtcttt ctggtcggcc agggcctgcg cggcgatcag gacgccgcgc
     4381 gcggcgaaag ccgtgcccgc gtagtcggtg cgcagcgtat cggccgccgc cgtgatgcgc
     4441 ttggtggcgt ccgcgccgcc cgggccgttc acgccgcggg ccgcgtcttc gagcgcctcg
     4501 aaataaccca tggcctggct ggcgcggtag ccgttgtagg cccgccagcc cgaccaaccc
     4561 gccgcggcca gcacgacgac cgtggccacc gacagggcca gggtgccgta gcggctccac
     4621 catgccttga ttgcgtcgag tttttcctgt tcttcgagat agtatgccat cgctaccaat
     4681 ccatcacttc gtattcagcg cggcttgcac cgcctcggcc agccgggcca gcggcacctg
     4741 ctgctggccg cccgcttcca gggtcgcgcc gcgcaaaaac ttgacgccgg ccgctttatt
     4801 ggcgacttcg tcgtcgccaa ggataaccgc aacccgcgcg ccgctgacgt cggcccgctt
     4861 gaactgcgac ttgaagctgc cggaaccggc atgcacgatg acggcgaggc cggcatcgcg
     4921 caggctctcg gcggcctggg ccgccaggcg ctgcgccgca tcgccctggt gcaccacgta
     4981 gacatcgcac tcgggcacgg gagcaggctg ctcgatgctg gcggccaggg tctgctccca
     5041 caggtcgagc aggcgctcca cgccgatggc gaagcccacc gcgggcgcgg ccttgccgcc
     5101 cagcagttcg atcaggccgt cgtagcggcc gccgccgcag accgtgccct gcgaacccag
     5161 gcggtcggtc acccactcga acacggtgag gttgtagtaa tcgaggccgc gcaccaggcg
     5221 cgggttcagg cggtattcga tgcccgcgtc ggccaggcgc tggcacacgc cgtcgaaatg
     5281 cgcgcgcgat tcgtcgccca ggaagtcgaa caggcgcggc gcgctgtcgg ccatgtcctg
     5341 catggcggga ttcttggtgt cgagcacgcg cagcgggttg gtgtagaggc ggcgcttgcc
     5401 gtcctcgtcg aggatgtcct ggtactgctc caggtgctcg atcagggcgg cgcgatgggc
     5461 cgcgcgttcg gccggctggc ccagcgagtt cagctccagg cgcacgtcgg ccaggcccag
     5521 gcgcttccac aggcgggcca gcatgacgat gagttcggcg tcgacgtccg gaccggcgaa
     5581 gcccagggcc tcggcgtcga tctggtggaa ctggcgatag cggccgcgct gcgggcgttc
     5641 gtggcggaac accgggccga cggagtagac gcgctgcgga cggtcgtaga gcaggttgtg
     5701 ctcgatgctg gcgcgcacga tgccggccgt catttcaggg cgcagcgtga gcgaatcgcc
     5761 gttgagcgag tcggtgaagg tgtacatctc cttctcgacg atgtcggtga cttcgccgat
     5821 gccgcgcgcg aacaggcgcg tgtgctcgag cacgggcgtg cgcacattgc ggtagccgta
     5881 gccgtgcagc cagtcgcgca cgatttcctc gaactgctcc cagcgcgcgc tgtcgccggg
     5941 cagaagatcg ttcatgccgc gtatggcggt gactttttga aaagcttgcg tcatctctcg
     6001 tcatggcctg cacgatgcgc gaacgccgcg caggccgtat ccttggttct tttaatgggt
     6061 ggccgcggcc gtgccgtagc ggcgcgccac gtagtcttcg acgatgacct ggaactcttc
     6121 ggcgatgcgg tcgcccttga gcgtgaccgt gcgttcaccg tcgacgaaca ccggcgcggc
     6181 cggcacctcg ccggtgcccg gcaggctgat gccgatgtcg gcatggcggc tttcgccggg
     6241 gccgttgacc acgcagccca tgacggccac gttcatgttc tcgacgccgg ggtattgcag
     6301 gcgccaggcc ggcatctggc ggcgcagata gccctggatg ctgtcggcca gttcctggaa
     6361 gacggtgctg ctggtgcgcc cgcatccggg gcaggccacc accaggggcg tgaaggcccg
     6421 tagacccatg gtctgcaaga tttcctgcgc gacgatgacc tcgcgggtgc ggtcgccgcc
     6481 cggttcgggc gtcagcgaga tgcggatggt gtcgccgatg ccttcctgca gcagcacggc
     6541 cagcgcggcc gtggaggcca cgatgccctt gctgcccatg ccggcctcgg tcaggcccag
     6601 gtgcagcggg tagtcgcagc gcgcggacag gtcgcggtag acggcgatga ggtcctgcac
     6661 gtggctgacc ttgcacgaca ggatgatggc gtcgcccggc aggccgagcg cttcggcgcg
     6721 ctgcgcgttg ctgatggccg acaccaccag ggcatcgcgc atcacggcct gggcttccca
     6781 gggctgggcg cggcgcccgt tgtcgtccat catgcgggcc atcagctcgt gatccaggct
     6841 gccccagttc acgccgatgc gcaccggctt ttcatagcgg caggctactt cgatcatctg
     6901 cgcgaagttg tcgtcgcggc gcttgccgcc ccccatattg ccggggttga tgcggtattt
     6961 ggacagggcc tgcgcgcact cgggaaactg cgtgagcagc ttgtggccgt tgtagtggaa
     7021 gtcgcccacc agcggcaccg acatgcccat gcggtcgagc tgctcgcgga tggcgggaac
     7081 ctcgcgggcg gcttcggggg tgttgacggt gatgcgcacc acctcggaac cggccagggc
     7141 cagttccttg acctggatgg ccgtggcgat ggcgtcggcc gtgtcggtgt tggtcatgga
     7201 ctgcaccatc accggcgcgc cgccgcccac ggtgaccaca cggtcgttcc agcggatatc
     7261 gacggcacgc gtgacgcggc gggcagccgg cccgacggcg ggcggcaggt cctggcaggg
     7321 cagagaggtt tcgtcttgca tggtcggcac cgtcctagtg gaaacgctgt ttgagcccgt
     7381 ccagggacaa cagctccttg ggcagccagc cttgccagaa ggcataggcc acgacggcga
     7441 tcacgacgac caggatcacc agcagccagc cccagggcac cgacgacgca tggggcgcgt
     7501 cctcgccgcc gacggcggcg ctggcgccca ggtcgcggcc ggtgggacgg atcgaaaccg
     7561 aggcatggct ggacagggcc tcgacgatgg gctccatgtc ggcgccgacc agccgggcgt
     7621 aattgcggat catgccgcgc aaggacacgc cggaaggcag ctggtcccag cgctcttcct
     7681 cgagcgcctg gatctggcgg gtggagtatt tcaagcgggc cgacacgtct tcgagcgaca
     7741 ggccgcgcgc aattcgcaaa tcccgcaagg cgctgcccac actcttcgtc tgggacgttt
     7801 cgtctacgac cgtggaatcc tgctggatca tgcactcacc tgtgtaatgg gtatggtgcg
     7861 cgacttgcgt tcggccagcc gggtgcggtc ctggacctcg ccggccaatt ggccgcatgc
     7921 ggcggcgatg tcgtcgccgc gcgtcttgcg caccgtggtg atgatgccgg catccatcag
     7981 ccgctgggcg aacacccgca cgcgcgcctg gggcgagcgc ttcaggccgg aagccgggaa
     8041 gggattgaat gggatcagat tgagcttaca gcggatttgc cgtgcgagct ggatgagttc
     8101 cttggcgtgc tggtcggtat cgttgatgcc gtccagcatg cagtattcga acgtgatgaa
     8161 atcgcgcggc gcgtaggcca ggtagttttc gcaagcctcg agcagcgatt tcagggggtg
     8221 cttgcggttg agcggcacga gctcgtcgcg cagggcgtcg ttgggcgcgt gcagcgaaac
     8281 ggccagcgcc acggggcagt cctgcgccag gcgatccatc atgggcacca cgcccgaggt
     8341 cgagaccgtg acgcggcggc gcgacaggcc ataggcattg tcgtcgagca tgaggcgcag
     8401 cgccggcacg agctggtcgt agttgagcag gggctcgccc atgcccatca tgaccacatt
     8461 gctgatgacg cgggggtctt gctgctcgcc ctcggacttg gcgccgccgg atttggcatc
     8521 acccgccggc aggcgggcgg agcccgcatc ggccacgagc acgcggcgcg cccaccacag
     8581 ctggccgacg atctcggccg tggtcaggtt gcggctgaag ccctggtgcc cggtggagca
     8641 gaagcggcaa ttgaccgcgc agcccgcctg gctggatacg cacagggtgc cgcggtcgtc
     8701 ctcggggatg aacaccgttt cgatggcgtt gccctgcccc acgtcgaaca gccacttgcg
     8761 cgtgccgtcg gacgatacct gctcggtact gaccggcagc gcctgcacgc ggcagcgctc
     8821 ggagagctgg gcgcggaaat cacgcgccag gtcggacatg gcgtcaaacg aatcaacccc
     8881 gcgctggtgg acccagcgtt gcaattgccg ggcgcgaaac ggcttgccac cccattgtcc
     8941 gaccagttcg gacagggcag cagcgtcttg gcccagcagg ttgatacgtt cgttttccat
     9001 ggcgtcttgc atggccgcga tggcaacatc gcggccatgc ccggggaatc aacggctgtg
     9061 gacgttgatt tcggggaaga agaaagcgat ttcgacggcg gcggtttcag gcgcgtcgga
     9121 gccgtgcacg gcgttggcat cgatgctgtc ggcgaagtcg gcgcggatgg tgcccttctc
     9181 ggccttcttg gggtcggtgg cgcccatcag gtcgcggttc ttcaggatgg cttcttcgcc
     9241 ttcgagcact tgcacgaaga ccgggcccga gaccatgaag tcgaccaggt ccttgaagaa
     9301 aggacgggcg ctgtgcacgg cgtagaagcg ctcggcgtcg gcgcgcgaga gctggcgcat
     9361 ctgggcggcg atgaccttca ggccggcggt ctcgaagcgg gcaatgatct ggccgatgac
     9421 gttcttggca acggcatcgg gcttgataat ggagagggta cgctgggcgg tcgactggat
     9481 ggttgtagtg gttgccatgg aaactccaat aaaaacaggc acttctaagc ggttacagta
     9541 acccgacatt ctaacatgcc ggcaagaatg caatacatct taaaatcagc ggtttactcg
     9601 accccaagga aacgccgggc cgccccaagt ttcctgtccc ccacgggggg cgggctgggc
     9661 gtcgcccatc cctgggggcc cttccctgcg agcccctgtt ttgccggaat acaccgcaat
     9721 gacccaagct gctgaatcga ccacccccgc caccgctgcc accgctgccg ccacggacag
     9781 ccccgaactc gccaagagct tcgaacccgc cgagatcgaa tcccgctggt acgcccagtg
     9841 ggagcaacgc ggctatttcc aggccggccg gcacgtcaag acggggacgg atgacaagtc
     9901 atactgcatc cagtttcccc cgcccaacgt caccggcacc ctgcacatgg gccacgcctt
     9961 caaccagacc atcatggacg gcctggtgcg ctaccaccgc atgtcgggcg acgacacggt
    10021 gttcatcccc ggcaccgacc acgccggcat cgccacccag atcgtggtgg aacgccagct
    10081 cgacgcgcag aaaatctcgc gccacgacct gggccgcgaa aaattcgtgg aaaaagtctg
    10141 gcagtggaag gagcagtccg gcaacaccat taccggccag gtgcgccgcc tgggcgcctc
    10201 ggccgactgg ccgcgcgaat atttcaccat ggacgagcgc atgtcgcgcg gcgtggtcga
    10261 gactttcgtc aagctctacc agcagggtct gatctaccgc ggcaagcgcc tggtgaactg
    10321 ggaccccaag ctgctgacgg ccgtgtccga cctggaagtc gtgtccgaag aggtcgacgg
    10381 cttcctgtgg cacatcgagt accccttcgt cgacggccct caaaccatcg tcgacaagga
    10441 cggcagcacc gtcacgctgc gcggcatgac catcgccacc acccgcccgg agaccatgct
    10501 ggccgacggc gcgctgtgcg tgcaccccga cgacccgcgc tacaagcacc tggtgggcaa
    10561 gctggtcgag ctgcccctgt gcgaccgcaa tatccccatc atcgccgacg acttcgtcga
    10621 catggatttc ggcaccggct gcctgaagat caccggcgcg catgatttca acgactatgc
    10681 ctgctcgcag cgccacaacc tgccgctgat caccatcttc acgctcgacg cgcacatcaa
    10741 tgaaaacggc cccaagcgct tccaggggct ggatcgcgac gcggcccgca aggccgtggt
    10801 ggccgcgctg gaagaagacg gcttcctggt caaggtcgtg ccgcacaaga tgatgcagcc
    10861 caagggcgac cgcaccggcg tcgtgatcga gcccatgctg accgaccagt ggttcgtcgc
    10921 catgagcaag cccgcgcccg acggcgccat caaccccggc cagagcatca cccaggtggc
    10981 gctggacgcc gtggccaacg gccgcatcgc cttctacccc gagaactgga ccaccatcta
    11041 caaccaatgg ctggagaaca tccaggactg gtgcatctcg cgccagctgt ggtggggcca
    11101 ccagatcccc gcatggtact cggaagacgg ccggatattc gtggccaaga gcgaggccga
    11161 ggccatcgaa caggcccgcg ccgccggcgt ggccggcccg ctcaagcgcg acgaggacgt
    11221 gctcgacacc tggttctcgt cggccctggt gcccttcacc accctgggct ggccggagaa
    11281 cacgctcgac taccagcgct acctgccctc cagcgtgctg gtcacgggtt tcgacatcat
    11341 cttcttctgg gtggcgcgca tggtgatgat gaccctgcac atgaccggcc agatcccctt
    11401 caggcacgtg tacgtgcacg gcctgatccg cgacgccgat ggccagaaga tgagcaagtc
    11461 caagggcaac acgctcgacc cggtcgacct gatcgacggc gtcgacctgg actcgctggt
    11521 cgccaagcgc accttcgggc tgatgaaccc caagcaggcc ggcacgatcg agaaaaccac
    11581 gcgccgccaa taccccgacg gcatcccggc cttcggcacc cacgcgctgc gcttcaccat
    11641 ggcggcctac gccacgctgg gccgcaacat caacttcgac ttgaagcgct gcgagggcta
    11701 ccgcaacttc tgcaacaagc tgtggaacgc cacgcgcttc gtgctgatga acaccgaagg
    11761 acacgacctg cacggcaagg aagagagccc ccacgccgcg ccctccgggc aagccgaact
    11821 gtcatttgcc gacaagtgga tcgtcagcct gctgcagggc ctggaagccg acgtggccaa
    11881 gggctttgcc gactaccgct tcgacaacat cgccaacgcg atctaccgct tcgtgtggga
    11941 cgagtactgc gactggtacc tggaactggc caaggtgcag atccagggcg gcacgccggc
    12001 ccagcagctg ggcacgcgcc gcacgctgat ccgcgtgctg gaagtggttc tgcgactggc
    12061 gcatcccatc atccccttca tcaccgaaga actgtggcag aaggtgtcgg tggtggcggg
    12121 caagcgccgc gccgacgagg ccggcagcgt gagcgtgcag ccctaccccg tcgccaaccc
    12181 ggccgcgatc gacgccgagg ccgaggccgc cgtggcggag ctgaagtccc agatcgaggc
    12241 ggtgcgcgcg ctgcgcggcg agatgagcct gtcgccggcg caacgcgtac cgttgttcgc
    12301 cgaaggcgat gcgcaagtgc tgggccgcaa cgcgccctac ctggcggcgc tggccaagct
    12361 gagccaggtg gatatcgtgg ccgccctgcc cgatgcgggc gccccggtgc aggtggtggg
    12421 caaggcgcgc ctgatgctgc a
//
LOCUS       DASWPO010000076        10257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1446125, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000076 DASWPO010000000
VERSION     DASWPO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446125"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGC69_04550"
     CDS             <1..346
                     /locus_tag="VGC69_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEY0294592.1"
                     /translation="FGGTAVVFVAMASLAGSVKRDLSNSLGKWLFAGVMVILVAGLAN
                     IFLHLPALMLTVSVLAIVIFSAFMLVDLQRIINGGETNYVTATLAVYLDVYNVFSNLL
                     MLLGIFGGNNRN"
     gene            549..1385
                     /gene="queF"
                     /locus_tag="VGC69_04555"
     CDS             549..1385
                     /gene="queF"
                     /locus_tag="VGC69_04555"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886006.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEY0294593.1"
                     /translation="MSLSAAPLGHRVAYPAEYDPSLLFPIARAANRAGLRLDAATLPF
                     HGADIWTAYELSWLDTKGKPKVAMAVFTVPADSPNLIESKSFKLYLNSFNQTRLDDIS
                     VLRERLARDLDAATGAPVALELIAPRDFGRQAIAELEGTDLDALDVEIDTYQPAPELL
                     RCAPGSRAGDMAEETLCSRLLKSNCPVTGQPDWASVQIRYRGRPIDHEALLKYIVSFR
                     QHAGFHEHCVERIHADIMRACAPAELTVYARYTRRGGLDINPWRSNAPRASVDTARTA
                     RQ"
     gene            complement(1453..2028)
                     /gene="pgsA"
                     /locus_tag="VGC69_04560"
     CDS             complement(1453..2028)
                     /gene="pgsA"
                     /locus_tag="VGC69_04560"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY0294594.1"
                     /translation="MPLNVPIILTWLRIAMIPLVVGLFYLPESWLAMPVRDAFAAFAF
                     IIAALTDWFDGWLARRWNQTSSFGAFLDPVADKLMVSAALLVLLDLNRVDVFIALIII
                     GRELAISALREWMATIGARASVAVHRLGKFKAAAQMVAIPCLLYGRVLFGVNLDWLGG
                     WLIVIAAVLTVWSMLYYMRRAWPVIREKAGQ"
     gene            complement(2081..3910)
                     /gene="uvrC"
                     /locus_tag="VGC69_04565"
     CDS             complement(2081..3910)
                     /gene="uvrC"
                     /locus_tag="VGC69_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY0294595.1"
                     /translation="MPDAFDLKQFLADLPHLPGVYRHLDEAGEILYVGKARDLKRRVS
                     SYFQKNLASPRIAQMVSKVARVEVTVTRSEAEALILENNLIKRLHPRYNILFRDDKSY
                     PYLQVTAHDWPRIAYYRGSTSRRGQYFGPYPNAWAVRETIQILQKVFRLRTCEDSVFA
                     NRSRPCLLHQIGRCSAPCVRALSQEDYAADVQRAVRFLNGEAQDVMGEIEARMLQASS
                     ELRFEEAGALRDQMGSLARVLHQQTMESVDGEDTDVIAVASAGGKICVNLAMVRGGRH
                     LGDKPFFPAHAEGESAEDVLTAFVAQHYVDGLVPPVLVATHALSEGELLGLLAEQAGV
                     KVPRLLTRPQGMRRAWLEQAQKNAELALARALSESGAKAARTLALAEALDLDSDEATL
                     DALRIECFDISHTAGEATQASCVVFQHHDMQPSLYRRYNIVGITPGDDYAAMRQVLTR
                     RFGKVADGESEMPGLVLIDGGKGQVEVARQVFVELGLDVQSLVGVAKGEGRKVGLETL
                     VFADARPPVALGKESAALMLIAQVRDEAHRFAITGMRAKRAKTRNVSRLEEIEGVGAR
                     RRQRLLARFGGLSGVISASVADLASVEGISDELAERIYDVLHS"
     gene            complement(3948..5018)
                     /gene="nagZ"
                     /locus_tag="VGC69_04570"
     CDS             complement(3948..5018)
                     /gene="nagZ"
                     /locus_tag="VGC69_04570"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY0294596.1"
                     /translation="MGQKSKSRKTRHVLPPGPVVVDVAGFKLTKEEKQRLRHPLVGGV
                     ILFARNFESREQLCALTRKIHEVRKEPLLIAVDHEGGRVQRFRGDGFTVLPPMRALGR
                     VWDADPLAAMRLACDAGYVLAAELRACGVDMSFAPVLDLDYGVSKVIGTRAFHRDPRV
                     VAMLARAVAQGLAMAGMAACGKHFPGHGFVGADSHHEIPVDPRSLERVMKDDAAPYGW
                     LGDGVLQAVMPAHVIYPKVDAQPAGFSRRWVQDILRGRLAYEGVVFSDDLTMEGAAVA
                     GDIVARSQAALGAGCDMVLVCNRPDLADDLLSRLSFDMPAQSVARVRGLMPRWTAPGW
                     DALQDEGRYQQARRAVAQIQAD"
     gene            complement(5049..5483)
                     /gene="acpS"
                     /locus_tag="VGC69_04575"
     CDS             complement(5049..5483)
                     /gene="acpS"
                     /locus_tag="VGC69_04575"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY0294597.1"
                     /translation="MSANPVPAPAAAGCIAGIGLDLIRIDRIERALARHGDRFAEKIL
                     GAEELQKFQTRRRRDQARGVRFLATRFAAKEAFSKAIGLGMRMPMYWNRVQVLNAPGG
                     RPMLVIGPALLDWYAARFGAAHVSLTDETDMAAAYVVVERKP"
     gene            5826..7130
                     /locus_tag="VGC69_04580"
     CDS             5826..7130
                     /locus_tag="VGC69_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294598.1"
                     /translation="MSTTSAVGQAAAASAPARRLNSPFQILTASLIGTTIEFFDFYVY
                     ATAAVLVFPTLFFPSSDPVTALLASFATFSIAFFARPIGALVFGHFGDRIGRKKTLIA
                     ALLTMGISTMVIGMLPSYHQAGVAAPLLLALCRFGQGFGLGGEWGGALLLATENATEK
                     NKSWYGMFPQLGAPVGLFLASGVFWVLMHFISQDALVSWGWRVPFLASALLFAVGLWV
                     RFSITETPEFEAAAKKNEQVAVPVMELFRHHKRSLFLGIFLGLITFVMFYIGSAYLLS
                     YNVKTAGMPFTEALTVQLYSSIVFGLSTIAAGLLGDKLGRRNVLIGTAVATGIFSFLV
                     SPLLTAGTAGIFAFALLGMLIFGVNYGVMGTALAAPFPTRVRYTGSSIAFNVSGILGA
                     SLAPYIATWLQIHYGLAAVGYYLLAAALISLYCMIVSRSESL"
     gene            complement(7191..8483)
                     /locus_tag="VGC69_04585"
     CDS             complement(7191..8483)
                     /locus_tag="VGC69_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294599.1"
                     /translation="MNTRKYWVIVWALFIGNVLNYVDRVNLSIAGPHIAKEFGFAPAT
                     MGFIYSSFFYSYILLILPLGMLADKYGSRVVSSAGLLIWSAGALATGAVVGLPSILLA
                     RLLLGSGEASTYPTSNRIMREWVPPRTRGLATSLFNIGSTAGPALGIASTSFLIAQTS
                     WRMSFYVLGGFTLIWAILWTVFYRSPESAKWLKDAERKYILDAREVAGVKVVKKMSLR
                     TLLEQRSMWGLVITHGCLTYSIYLFLTWLPSYLMDVRGLTIMSAGLVGTLPYIISSAG
                     TILIGSLSDRYMSNKDVSRGGRKWLVILMLALSSCIFFVPVVESTFLLVAIISASILF
                     ATAANTMNLALTGDLIVDKGSAGAAYGLLILGGNTFGFLAPLITGYVIQVTHSYTWSF
                     IIAGLFMVAGIISLSLVNRPLQPADASTFSSEKLAGPA"
     gene            complement(8514..9416)
                     /locus_tag="VGC69_04590"
     CDS             complement(8514..9416)
                     /locus_tag="VGC69_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0294600.1"
                     /translation="MTISETSSPPRAPNILAPLAPARTPSTKFPKNSCDCHAHVFGNQ
                     EKYPLRSDATYIPHVSPVEDYIHMLESIGCDRAVLVQPSGYGADNRCLVDTLNSGLFD
                     FRGVAVVDPAVTAAEIEALHRVGVRGIRINTTSSASAISLRDAGEMARLILPYGWHIQ
                     FYIEPSHIEEVAATVKRLPVNCVIDHFAQIDADQVPQPPAFRTLMEMSLWDNVWFKLT
                     GGYRVSHEPPPYPRVKDFVQALLVNAADRCVWGSDWPHPLVSRMDDDAAIADALGFWV
                     EDEHIRNTILVTNPARLYDFSTNR"
     gene            complement(9489..>10257)
                     /locus_tag="VGC69_04595"
     CDS             complement(9489..>10257)
                     /locus_tag="VGC69_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0294601.1"
                     /translation="ASAHDRVPAYASGGYYLPGKTPDKLAQEMVDHVKDGFAAVKMKV
                     GLLSVAEERKRIAAVREAIGEGVRLMLDANNAWRDLPTALEYMHAFEPYQPFWIEEPF
                     SPDDIDNHARLARVTPVTVATGEVEAGRWRFTDLIVRGATTLLQTDATVCGGITEWRR
                     VAATAASHGLSVSPHAWHDVHVHLVGATPNATYAEWMPDDHIVNFRRLIDRQLVAEHG
                     DLLLPQEPGLAFRFDDAAIARYGVVYPGERDRWITVA"
BASE COUNT         1813 a         3532 c         3162 g         1750 t
ORIGIN      
        1 cttcggcggc acggcagtcg tgttcgtcgc catggcctcg ctggccggca gcgtcaagcg
       61 cgatctctcc aacagcctgg gcaagtggct gttcgccggc gtcatggtca tcctggtcgc
      121 gggcctggcc aacatcttcc tgcacctgcc cgcgctcatg ctcaccgtct cggtgctggc
      181 catcgtgatc ttctcggcct tcatgctggt cgacctgcag cgcatcatca acggcggcga
      241 aaccaactac gtcaccgcca ccctggccgt gtacctggat gtctacaacg tgttcagcaa
      301 cctgctgatg ctgctgggca tcttcggcgg caacaaccgg aactgagcca aggcgccctg
      361 aagccgtcga cgtcgaatta ggctgacgaa agccgaggac cccggagaaa tccgaagcac
      421 aggcgcgaga gcgccgagca tcggaatttc gcagggcagc ctcggcggct caagaacttg
      481 aatcggcacc cgaaacaggc ctctacaatg aggcctgttc gttttttgca cgcacgcgcc
      541 aatacatcat gtcgctatcc gccgccccgc tgggccaccg cgtagcctat cccgccgagt
      601 acgacccctc gctgctcttt cccatcgcgc gcgcggccaa ccgggccggc ctgcgcctgg
      661 atgcggccac cctgcccttc catggcgccg acatctggac agcctacgaa ctgtcctggc
      721 tggacaccaa aggcaagccc aaggtggcca tggccgtctt caccgtgccg gccgacagcc
      781 ccaatctcat cgaatccaaa tcgttcaagc tctacctgaa ctcgttcaac cagacccgcc
      841 tggacgatat ctcggtgctg cgggaacggc tcgcgcgcga cctcgacgcc gccaccggcg
      901 caccggtggc gctggaactc atcgccccgc gcgacttcgg ccgccaggcc atcgccgagc
      961 tcgaaggcac cgacctggac gcgctcgacg tcgagatcga cacctaccag cccgcccccg
     1021 aactgctgcg ctgcgcgccc ggctcgcgcg ccggcgacat ggccgaggaa acgctctgct
     1081 cgcgcctgct caagtccaac tgcccggtca ccggccagcc cgactgggcc agcgtgcaga
     1141 tccgctaccg gggccgcccc atcgaccacg aggccttgct caaatacatc gtgtccttcc
     1201 gccagcacgc gggctttcac gagcactgcg tcgagcggat ccacgccgac atcatgcggg
     1261 cctgcgctcc cgccgagctt accgtctacg cccgctatac gcggcgcggc gggctcgaca
     1321 tcaacccgtg gcgcagcaat gcgccacggg catcggtcga taccgcgcgc acggcgcggc
     1381 aatgatgcgc gatacagcaa ggccgcaata aaaaatcggg cctggcgtga cggcacccca
     1441 ggcccgattt ttttactgcc ccgccttttc ccgtatcacc ggccaggcgc gccgcatgta
     1501 atacagcatc gaccacaccg tcagcacggc ggcgatcacg atcagccagc cgcccagcca
     1561 atccaggttc accccgaaca gcacccggcc gtacaacagg cagggaatcg ccaccatctg
     1621 cgcggccgcc ttgaacttgc ccagccgatg caccgccacg ctggcgcggg cgccgatcgt
     1681 ggccatccac tcgcgcaggg ccgaaatggc gagttcgcgg ccgatgatga tcagcgcgat
     1741 gaagacatcc acccggttca ggtccagcag caccagcagc gcggcgctga ccatgagctt
     1801 gtcggccacc ggatcgagga aggcgccgaa cgacgaggtc tggttccagc ggcgcgccag
     1861 ccagccgtcg aaccagtcgg tcagcgcggc aatgatgaag gcaaaggcgg cgaaggcgtc
     1921 gcgcaccggc atggccagcc agctttccgg cagataaaac aggcccacca ccagcgggat
     1981 catggcgatg cgcaaccagg tcaagatgat cggtacgttt agcggcatag tgaacgtatg
     2041 ctacatcagg cccggttaca tctcatacat caccggcctg ctagctgtgc agcacgtcgt
     2101 aaatgcgctc ggccagttcg tcggaaatcc cttccaccga ggccaggtcg gccacgctgg
     2161 ccgagatcac gcccgacagc ccgccaaagc gcgccagcag ccgctgccgc cggcgcgcgc
     2221 ccacgccctc gatctcttcc aggcgcgaca cattgcgggt cttggcgcgc ttggcgcgca
     2281 tgccggtgat ggcgaagcgg tgcgcctcgt cgcgaacctg ggcgatcagc atcagcgcgg
     2341 cggattcctt gcccagggcc acgggcgggc gggcgtcggc aaacaccagg gtttccaggc
     2401 ccaccttgcg gccctcgccc ttggccacgc ccaccaggga ctggacatcc agtcccagct
     2461 cgacgaagac ctgccgggcc acctcgacct ggcccttgcc gccgtcgatc agcaccaggc
     2521 cgggcatctc cgactcgccg tcggccacct tgccgaagcg gcgcgtcagc acttggcgca
     2581 tggcggcgta gtcgtcgccg ggcgtgatgc cgacgatgtt gtagcgccga tacagggagg
     2641 gctgcatgtc gtggtgctgg aacaccacgc acgaggcctg ggtcgcctcg cccgccgtgt
     2701 ggctgatgtc gaagcactcg atgcgcaggg catccagcgt ggcctcgtcg gagtccaggt
     2761 ccagcgcttc ggccagggcc agggtgcggg ccgccttggc gccggattcg ctgagcgccc
     2821 gggcgagcgc cagttcggca tttttctgcg cctgttccag ccaggcgcgc cgcatgcctt
     2881 gcggacgcgt caagaggcgc ggcaccttca cgcccgcctg ctcggccagc aggcccagca
     2941 gctcgccttc ggacagggca tgcgtggcca ccagcaccgg cggcaccagg ccgtccacgt
     3001 agtgctgcgc cacgaaggcg gtcagcacgt cctcggccga ttcgccctcg gcatgggccg
     3061 ggaagaaggg cttgtcgccc aggtgccggc cgccgcgcac catggccagg ttcacgcaga
     3121 tcttgccgcc ggcgctcgcc accgcgatca cgtcggtatc ctcgccgtcg acgctctcca
     3181 tggtctgctg gtgcagcacg cgggccagcg accccatctg gtcgcgcaag gcgccggcct
     3241 cttcgaagcg cagttcgctc gaggcctgca gcatgcgcgc ctcgatctcg cccatcacgt
     3301 cctgcgcctc gccgttcagg aagcgcaccg cgcgctgcac atcggccgcg tagtcttcct
     3361 ggctgagcgc ccgcacgcac ggcgccgagc agcgcccgat ctggtgcagc aggcagggcc
     3421 gcgagcggtt ggcgaacacc gagtcctcgc aggtgcgcag gcggaacact ttctgcagga
     3481 tctggatcgt ctcgcgcacc gcccaggcat tgggataagg gccgaagtac tggccgcggc
     3541 gcgaggtgga cccgcggtaa taggcgatgc gcggccagtc gtgcgccgtc acctgcagat
     3601 agggatagga cttgtcgtcg cggaaaagaa tgttgtagcg cgggtgcagg cgcttgatca
     3661 ggttgttttc caggatcagc gcctcggcct ccgagcgcgt caccgtcacc tcgacccggg
     3721 ccaccttgga caccatttgc gcgatgcgcg ggctcgccag gtttttctgg aaatacgagg
     3781 acacgcgccg cttcaggtcg cgcgccttgc ccacgtacag gatctcgccg gcctcgtcca
     3841 gatgccggta gacgcccggc aggtggggca gatccgccag gaattgcttg aggtcgaatg
     3901 cgtcgggcat gctcgttgct gcgtcgtggc tacagggccg cggcccgtca atccgcctgg
     3961 atctgcgcca ccgcgcgccg ggcctgctgg tagcggccct cgtcctgcag cgcgtcccag
     4021 ccgggcgccg tccagcgcgg catcaggccg cgcacgcgcg ccacggactg cgcgggcatg
     4081 tcgaaggaca ggcgcgacag caggtcgtcg gccagatcgg gccggttgca taccagcacc
     4141 atatcgcagc ccgcgcccag cgcggcctgc gaccgcgcga cgatgtcgcc ggccacggcg
     4201 gcgccttcca tggtgaggtc gtccgagaac accacgcctt cataggccag gcggccgcgc
     4261 aggatgtcct gcacccagcg ccgcgagaag ccggccggct gcgcatccac cttgggatag
     4321 atcacgtgcg ccggcatcac ggcctgcagc acgccgtcgc ccagccagcc gtagggcgcc
     4381 gcatcgtcct tcatcacgcg ttccagcgag cgggggtcga ccggaatctc gtggtgcgag
     4441 tccgccccga cgaagccatg ccccgggaag tgcttgccgc acgcggccat gccggccatg
     4501 gccaggccct gggccaccgc ccgggccagc atggccacca cccgcggatc gcggtggaaa
     4561 gcgcgcgtgc cgatcacctt gctcaccccg tagtccaggt cgagcaccgg cgcgaagctc
     4621 atgtccacgc cgcaggcgcg cagctcggcg gccagcacgt agccggcatc gcaggccagg
     4681 cgcatggccg ccagcgggtc ggcgtcccag acccggccca gcgcccgcat gggcggcagg
     4741 accgtgaagc catcgccgcg aaagcgctgc acgcgcccgc cctcgtgatc caccgcgatc
     4801 agcaacggct ccttgcggac ttcgtggatc ttgcgcgtca gcgcgcaaag ctgctcgcgg
     4861 ctctcgaaat tgcgggcgaa cagaatcacc ccgcccacca gcggatggcg caggcgctgc
     4921 ttttcttcct tggtgagctt gaagcccgcc acgtcgacca ccacgggccc ggggggcagg
     4981 acatgcctgg ttttccggct cttgcttttc tgacccatgc gagcctcctg tctgttgcgt
     5041 gcgcagccct agggcttgcg ctcgaccacc acataggcgg cggccatgtc ggtttcgtcg
     5101 gtcagcgaca catgcgccgc gccgaatcgc gcggcatacc agtccagcag cgcgggaccg
     5161 atcaccagca tcgggcgccc gccgggcgcg ttgagcacct gcacgcggtt ccagtacatg
     5221 ggcatgcgca tccccaggcc gatggccttg gagaaagcct ccttggcggc aaagcgcgtg
     5281 gccaggaagc gcacgccgcg cgcctggtcg cgccggcggc gcgtctggaa cttctggagt
     5341 tcctcggcgc ccaggatctt ctcggcaaag cgatcgccat gccgcgccaa ggcccgctcg
     5401 atgcggtcga tgcggatcag atccaggccg ataccggcaa tgcagccggc cgcggcgggc
     5461 gcggggacag ggtttgctga catggcggaa tcatacgcga gtcggggacg ccggcccggc
     5521 cctgctacac tatggaaaac aacagccgcc tgcaacacca gggcggacaa acgcagcagc
     5581 attctgccgt ggcgccccgc ccggcatcgc gcacctcccg cgggccaccc ctgccgggac
     5641 gcgtcttgct cgatttccgt cccgctgtcc tatcccacgc ggctgttcca gcctcgctcg
     5701 cgctccaaca ggaacgaggc gccttccgca tgcggcagcc acaagcagcc gagtgcctcc
     5761 acgacggcgc cgggctggat cgactctccc cgtcctcatt ccgttctcat acgcgccgca
     5821 tcatcatgtc cacgacttcc gcagtcggcc aggccgccgc agcgtccgct cccgcccgcc
     5881 gcctcaattc gcctttccag atcctcaccg ccagcctgat cggcaccacc atcgagttct
     5941 tcgatttcta cgtctacgcc acggcggccg tcctggtgtt tcccacgctg ttcttcccgt
     6001 ccagcgatcc cgtcacggcg ctgctggcct cgttcgccac cttctccatc gcctttttcg
     6061 cgcgcccgat cggcgcgctg gtcttcggcc acttcggcga ccgcatcggc cgcaagaaaa
     6121 cgctgatcgc cgccctgctc accatgggca tctcgaccat ggtgatcggc atgctgccct
     6181 cctaccacca ggccggcgtc gcggcgccgc tgctgctggc gctgtgccgt ttcggccagg
     6241 gcttcggcct gggcggtgaa tggggcggcg cgctgctgct ggccaccgag aacgccaccg
     6301 aaaagaacaa gagctggtac ggcatgttcc cgcagctggg cgcgccggtc ggcctgttcc
     6361 tggcatcggg ggtcttctgg gtgctgatgc acttcatctc gcaggacgcg ctggtgtcct
     6421 ggggctggcg cgtgcccttc ctggcctcgg cgctgctgtt cgccgtggga ctgtgggtgc
     6481 gtttctcgat caccgaaacg cccgagttcg aagccgccgc caagaagaac gaacaggtcg
     6541 ccgtgccggt gatggagctg ttccgccatc acaagcgcag cctgttcctg ggcattttcc
     6601 tgggcctgat caccttcgtg atgttctaca tcggctcggc ctacctgctc tcgtacaacg
     6661 tgaagacggc cggcatgccc ttcaccgagg cgctcaccgt ccagctgtac tcgtcgatcg
     6721 tgttcggcct ctcgaccatc gccgccggcc tgctcggcga caagctgggc cgccgcaacg
     6781 tgctcatcgg cacggccgtg gccacgggca tcttctcgtt cctggtcagc ccgctgctca
     6841 cggcgggcac ggccggcatc ttcgccttcg ccctgctggg catgctgatc ttcggcgtga
     6901 actacggcgt gatgggcacc gcgctggccg cccccttccc cacccgcgtg cgctacaccg
     6961 gctcgtccat cgccttcaac gtatcgggca tcctgggcgc ctcgctggcc ccctacatcg
     7021 cgacctggct gcagatccat tacggcctgg ccgccgtggg ctactacctg ctggccgccg
     7081 cgctgatcag cctgtactgc atgatcgtct cgcgcagcga atcgctgtag gcggacctgc
     7141 cgcccgcgca ccggagcgca cgggttcttt tgccgccaga ccgggccact ctaggctggc
     7201 ccggccaatt tttcagaaga gaaggtcgac gcatcggccg gttgaagcgg cctgttgacc
     7261 aaggataggg aaatgatccc tgccaccatg aacagcccgg ctatgatgaa actccaggta
     7321 tatgagtgag tcacctggat tacatacccg gtaatcaacg gagccaggaa cccgaacgta
     7381 ttcccgccca ggatcaacaa tccgtacgcg gcacccgccg aacccttgtc gacgatcaga
     7441 tcgcctgtca aggccaggtt catcgtattc gccgcggtcg caaacagaat ggaagcgctg
     7501 atgatcgcga ccagcaggaa tgtgctttca acgacgggaa cgaaaaagat acaactggaa
     7561 agcgccagca tcaggatgac gagccatttt ctgccgccgc gagatacgtc tttgttcgac
     7621 atataccggt cgctcagcga accaatcagg atcgtcccgg cgctggatat gatgtaaggc
     7681 aaggttccta ccaaccccgc cgacatgatc gtcagccctc ggacatccat gagataggag
     7741 ggcagccagg tgagaaaaag atatatggag taggtcaggc acccatgggt aatgaccagc
     7801 ccccacatgg accgctgctc caaaagcgtt cgcagcgaca tcttcttcac gactttgacg
     7861 ccggctacct cgcgagcatc cagaatatat tttcgttcag cgtccttcag ccatttcgcg
     7921 gactccgggg atctatagaa tacggtccag aggatggccc agatcagcgt gaatccgccg
     7981 agaacgtaaa aagacatgcg ccagcttgtc tgggcgatca gaaaggaggt cgaggcaatc
     8041 cccagcgcgg gaccggctgt cgagccgata ttgaacaagg acgtcgccag ccctcttgtc
     8101 cttggcggca cccattcgcg catgattcga ttcgacgtgg gataggtcga ggcttcgccc
     8161 gagccgagca gaaggcgggc gagaagaatc gacggcaaac ccacgacggc gccggttgca
     8221 agggcgcccg ccgaccagat caacagccct gcggaagaga ccacccttga tccgtatttg
     8281 tccgccagca tgcccagcgg gagaataagc aggatatagc tgtaaaaaaa ggatgaatat
     8341 ataaaaccca tcgtcgcagg ggcgaaaccg aactcctttg cgatatgtgg cccggcgatg
     8401 gacagattga ctctatcgac ataattcaga acgttgccta tgaacagcgc ccagacaatt
     8461 acccagtatt tcctggtatt cacggattcc tctcctctac ctgctctttg cttttatctg
     8521 tttgtcgaaa agtcgtacag ccgggcggga ttggtcacca ggatagtgtt ccgaatgtgt
     8581 tcatcctcca cccaaaaacc aagtgcgtcg gcaatggcgg cgtcgtcgtc cattctggag
     8641 acgagcggat gcggccaatc cgaaccccat acgcacctgt cagccgcatt gaccagaagg
     8701 gcttgcacaa aatctttaac cctgggatag ggcgggggct catggctgac tctgtagccg
     8761 ccggtcaact tgaaccagac gttatcccaa agactcattt ccattaatgt ccggaacgcc
     8821 gggggttggg gtacctggtc ggcgtcgatt tgcgcgaagt gatcgatgac gcagttcacg
     8881 ggaaggcgct tcacggtcgc cgcgacctct tcgatatggc tgggctcgat gtagaactgg
     8941 atgtgccagc catagggaag gatgagcctt gccatctcac cggcatcacg cagagagatc
     9001 gcgctcgcac tggaggtcgt attgatccgg atgccccgta ctccgacgcg atgcagtgcc
     9061 tcgatttccg cggccgtaac ggccgggtcc accaccgcca cgccgcggaa atcaaaaagt
     9121 ccggaattta gcgtatcgac aaggcagcgg ttgtctgctc cgtagccgct gggttgaacc
     9181 agaacggcgc gatcgcaacc gattgactcc aacatgtgga tgtagtcttc aacgggcgaa
     9241 acatgcggga tgtaggtcgc gtcgcttctg agcggatatt tttcctgatt tccaaataca
     9301 tgcgcatggc agtcgcagga attcttcgga aatttcgtgc tcggcgttct cgccggagcc
     9361 agaggggcca ggatgtttgg ggccctaggc ggggaggatg tttcggaaat ggtcatttgg
     9421 gccaccaaaa aagctgcgag cagcgctgcg accgctctgc agagattgct tgaatcgacg
     9481 cacgcctgtc atgcgaccgt aatccatcga tcccgttcgc ccggatacac cacgccgtag
     9541 cgcgcgatgg ccgcatcgtc gaagcggaag gccaggcccg gctcctgggg cagcagcaag
     9601 tcgccgtgtt cggcgacgag ctgacggtca atcaggcggc ggaaattaac gatatggtcg
     9661 tccggcatcc actcggcgta ggtggcattc ggtgtggcgc cgaccaggtg cacgtgtacg
     9721 tcgtgccagg cgtgcggcga gacgctcagg ccgtggctgg ccgccgtggc ggccacgcgc
     9781 cgccattcgg tgatgccgcc gcacacggtg gcgtcagtct gcaggagcgt ggtggcgccg
     9841 cgcacgatca gatcggtaaa gcgccagcgg ccggcctcca cctcgccggt ggccacggtg
     9901 acaggcgtga cgcgcgccag ccgggcgtga ttgtcgatgt cgtcgggcga aaacggttcc
     9961 tcgatccaga acggctggta gggctcgaac gcgtgcatgt attccaacgc ggtaggcagg
    10021 tcgcgccagg cgttattggc gtcgagcatc agccgcactc cctcgccgat ggcctcgcgc
    10081 acggcggcga tgcgcttgcg ctcctcggcc acgctgagca gacccacttt catcttcacg
    10141 gccgcgaacc catccttcac atggtcgacc atctcttgcg ccagtttgtc gggcgtctta
    10201 ccgggcagat agtagccacc gctggcatag gcgggcacgc ggtcatgcgc cgacgcg
//
LOCUS       DASWPO010000077         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_554098, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000077 DASWPO010000000
VERSION     DASWPO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554098"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2058
                     /locus_tag="VGC69_04600"
     CDS             <1..>2058
                     /locus_tag="VGC69_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY0294602.1"
                     /translation="PEARFGIMTSGKAYLDTRQALADLGLSDDICRRIGLRLFKVGMV
                     WPLEATGMQQFAEGLDEILVVEEKRQVLEYQLKEELFGWIGTGRKIPRVVGKFDDKDG
                     GEWAVPQGNWLLPAHYEFSPAIVARAIGVRLLKFDLPDDVRAGIQARLAFLDAREKEL
                     SRPRVVAERKPWFCSGCPHNTSTRLPDGSRGMAGIGCHYMVTWMDRNTQVFTQMGGEG
                     VPWIGQAPFTEEKHVFANLGDGTYYHSGLLAIRAAVAAKASITYKILFNDAVAMTGGQ
                     PVDGPITVPMITRQMAAEGIEKIIIVTDEPEKYKGVTDLAPGVPVKHRDELDGVMREL
                     REYGNVSILIYDQTCATEKRRRRKRNAYPDPARRVVINDRVCEGCGDCSEQSHCLSVE
                     PLETELGRKRVINQSSCNKDFSCLKGFCPSLVTVEGGKLRKPKALAQEGGIADGLPEP
                     KLPDLSALDSGASHYGVFIAGVGGTGVVTIGQLIGMAAHIEGKGCSVLDMAGLAQKGG
                     AVFSHVVLAPTPGELLNTRVAMGEADLALLGDLVVGTSQEAIARMLPGRTRVLLNSDV
                     APTAGFVHNPDWTLPGADLRADLAAACGQGNLAAIDAAELAVGLLGDAIYANPMMMGY
                     AYQKGWLPLGEASLKRAIELNGQQVPNNLAAFDWGRRAAHDLAAVQRLIGKGAAAPQI
                     QGDA"
BASE COUNT          341 a          729 c          706 g          282 t
ORIGIN      
        1 cccgaggcgc gcttcggcat catgacctcg ggcaaggcct acctggacac gcgccaggcg
       61 ctggcggacc tgggcctctc cgacgacatc tgccgccgca tcggcctgcg cctgttcaag
      121 gtgggcatgg tgtggccgct cgaagccacc ggcatgcagc agttcgccga gggcctggac
      181 gagatcctgg tggtcgagga aaagcgccag gtgctcgaat accagctcaa ggaagagctg
      241 ttcggctgga tcggcacggg ccgcaagatc ccgcgcgtgg tcggcaagtt cgacgacaag
      301 gacggcggcg aatgggccgt gccgcaaggc aactggctgc tgccggcgca ctacgagttc
      361 tcgcccgcca tcgtggcccg ggccatcggc gtgcgcctgc tgaagttcga cctgcccgac
      421 gacgtgcgcg ccggcatcca ggcgcgcctg gccttcctcg acgcgcgcga gaaagaactg
      481 tcgcgcccgc gcgtggtggc cgagcgcaag ccctggttct gctcgggctg cccgcacaac
      541 acctccaccc gcctgcccga cggctcgcgc ggcatggcgg gcatcggctg ccattacatg
      601 gtcacctgga tggaccgcaa tacgcaggtc ttcacccaga tgggcggcga gggcgtgccc
      661 tggataggcc aggcgccctt caccgaggaa aagcacgtct tcgccaacct gggcgacggc
      721 acctactacc actcgggcct cctggcgatc cgcgcggcgg tggcggccaa ggcgtccatc
      781 acctacaaga tcctcttcaa cgacgccgtg gccatgacgg gcggccagcc ggtcgacggc
      841 cccatcaccg tgcccatgat cacgcgccag atggcagccg agggcatcga gaagatcatc
      901 atcgtcaccg acgagcccga gaagtacaag ggcgtcaccg acctggcgcc gggcgtgccg
      961 gtcaagcacc gcgacgagct cgacggcgtg atgcgcgagc tgcgcgaata cggcaatgtg
     1021 tccatcctca tctatgacca gacctgcgcc accgagaagc gccgccgccg caagcgcaac
     1081 gcctatcccg acccggcgcg ccgcgtggtc atcaacgacc gcgtgtgcga gggctgcggc
     1141 gactgctccg agcaatcgca ctgcctgtcg gtcgagccgc tcgaaaccga gctgggccgc
     1201 aagcgcgtca tcaaccagtc cagctgcaac aaggacttct cctgcctgaa gggtttctgc
     1261 cccagcctgg tgacggtcga gggcggcaag ctgcgcaaac ccaaggcgct ggcgcaggaa
     1321 ggcggcatcg ccgacggcct gcccgagccg aaactgcccg acttgtcggc gctggacagc
     1381 ggcgccagcc actacggcgt cttcatcgcc ggcgtgggcg gcacgggcgt ggtcaccatc
     1441 ggccagctca tcggcatggc cgcgcacatc gaaggcaagg gctgttcggt gctcgacatg
     1501 gccggcctgg cgcaaaaggg cggcgcggtg ttctcgcacg tggtgctggc gcccacgccg
     1561 ggcgagctgc tcaatacgcg cgtggccatg ggcgaggccg acctggcgct gctgggcgac
     1621 ctggtggtgg gcaccagcca ggaggccatt gcccgcatgc tgccgggccg cacgcgcgtg
     1681 ctgctcaaca gcgacgtggc gccgaccgcg ggcttcgtgc acaaccccga ctggacgctg
     1741 cccggagccg acctgcgcgc cgacctggcg gccgcgtgcg gccagggcaa cctggccgcc
     1801 atcgacgcgg ccgagctggc ggtgggcctc ctgggcgacg ccatctacgc caaccccatg
     1861 atgatgggct atgcctacca gaagggctgg ctgccgctgg gcgaggcgtc cttgaagcgc
     1921 gcgatcgagc tcaacggcca gcaggtgccc aacaacctgg ccgccttcga ttggggccgc
     1981 cgcgccgcgc acgacctggc cgcggtgcag cgcctgatcg gcaagggcgc tgccgcgccg
     2041 caaatccagg gcgatgcg
//
LOCUS       DASWPO010000078         9046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1535555, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000078 DASWPO010000000
VERSION     DASWPO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535555"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGC69_04605"
     CDS             complement(<1..362)
                     /locus_tag="VGC69_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0294603.1"
                     /translation="MNRYPLWKYITVLVAVVIGLLYTLPNFYGESPAVQVSSAKAVVK
                     VDPDLVDQIQQILSDAKLPPESIYYEQNGTLGTIRARYANTDQQLQARDAIDHALNTD
                     PSDPRYTVALNLLPASPT"
     gene            complement(460..801)
                     /gene="yajC"
                     /locus_tag="VGC69_04610"
     CDS             complement(460..801)
                     /gene="yajC"
                     /locus_tag="VGC69_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0294604.1"
                     /translation="MSVLNTATLVLGQAAAEGNPIMSMLPILLMFVILYFLMIRPQMK
                     RQKESRNMIATLSKGDEIVTSGGMLGKVTKVTDSYITAEVSELADKPVEVVLQKTAVT
                     TVLPKGTIKAL"
     gene            complement(1025..2161)
                     /gene="tgt"
                     /locus_tag="VGC69_04615"
     CDS             complement(1025..2161)
                     /gene="tgt"
                     /locus_tag="VGC69_04615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0294605.1"
                     /translation="MTGLNFELLATDGAARRGRMTLNHGVVETPIFMPVGTYGSVKGM
                     LPHELKDIGSQIVLGNTFHLWLRPGTEVLDKHGGLHGFMQWDKPLLTDSGGFQVFSLQ
                     GMRKITEEGVKFASPIDGARLFLTPEESMRIQKSLNSDIAMVFDECTPYEIKGRPATL
                     DEAAGSMRMSLRWARRSKDEFDRLGNPNALFGIVQGGMFEALRDESLAGLADIGFHGY
                     AIGGLSVGEPKQDMMRILSHVAPRLPADRPRYLMGVGTPEDLVDGVARGIDMFDCVMP
                     TRNARNGWLFTRHGDLKIRNARYRDDTRPLDPACRCHTCLNFSRAYLHHLQRANEISG
                     ARLNTLHNLHFYLDLMKQMREAIAEGRFDAWRAEFAQDRAKGID"
     gene            complement(2158..3276)
                     /gene="queA"
                     /locus_tag="VGC69_04620"
     CDS             complement(2158..3276)
                     /gene="queA"
                     /locus_tag="VGC69_04620"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY0294606.1"
                     /translation="MQLKISGSPPSPHNVPAFPSPAFTLADFDYPLPDELIAQHPAAE
                     RRGSRLLHLDGAGRLHDRQFPDLAGLVRENDLLVFNDTRVIKARLLGQKATGGKVEVL
                     VERITEPTRALAHVRASKSPGPGMILRLADAFDTEVLGRQGELFDLRFPGPVLDLLDA
                     HGATPLPPYITHDAQADDDSRYQTVYAREPGAVAAPTAGLHFDQAMLDQLAAQGARRA
                     FVTLHVGAGTFQPVRAANLADHIMHAEWYTVPQATVDAIAQTRARGGRVIAVGTTSVR
                     ALESAAAACATAAGQPLAAAQGDTRLFITPGYPYQVVDALVTNFHLPQSTLLMLVSAL
                     AGVEPIRRAYAHAVEQRYRFFSYGDAMFIESAGTPPRP"
     gene            3530..4951
                     /gene="dacB"
                     /locus_tag="VGC69_04625"
     CDS             3530..4951
                     /gene="dacB"
                     /locus_tag="VGC69_04625"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY0294607.1"
                     /translation="MHISGFKTWLAAVAAALCVGQVGAQALPANLAAAWQQTGLPENA
                     MSLVVQEVGGPRLAAVNAGVARNPASVMKLVTTWAALSELGPTYVWQTELVSEPGAAV
                     SNGVLSGPLYVRASGDPQLMVQDLWTLLRDLRLRGVRQLGDVVVDRSLFGNVATDPGA
                     FDNAPDRAYNASPDAFMVGFGAERLMFMADAPARAWRVVMDPALPNVKLEGRLAWSDA
                     PCQGSPEVNTLPVATPQGTTLRLSGTMPGACGDFSLYRLALTQPGHAAGVFRQIWQEL
                     GGTMTGQLRDGLAPVDGEVLASHDSPELGETLRTINKRSNNVMARTLLLTLGAERGRR
                     PATVASSDDVARRVLAGQGLLMPELVLDNGAGLAREARVSADSLAALLRTAWSSPYMP
                     EYVASLAIIGEDGTVRKRLRDGAAAGRAHLKTGSLRDVRAIAGYVTGVSGKRYIVVCI
                     VNHEQAAAVRSFDDALVDWLARR"
     gene            5011..5649
                     /gene="upp"
                     /locus_tag="VGC69_04630"
     CDS             5011..5649
                     /gene="upp"
                     /locus_tag="VGC69_04630"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY0294608.1"
                     /translation="MPVHEIRHPLIRHKLGLMRRADLSTKGFRELAGELGALLTYEAS
                     KDLPLCPAEVEGWNGTVQVEKLAGKKITVVPILRAGLGMLDGVLSLIPGAKVSAVGLA
                     RNEATLQAHTYLEKLVGELDQRIALIVDPMLATGGSMLATVDLLKRAGARDVRALVLV
                     AVPEGIRNVLDKHPDVQIYTACIDQGLNEHGYILPGLGDAGDRIFGTKQKAD"
     gene            complement(5674..7071)
                     /gene="cysG"
                     /locus_tag="VGC69_04635"
     CDS             complement(5674..7071)
                     /gene="cysG"
                     /locus_tag="VGC69_04635"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039051507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEY0294609.1"
                     /translation="MKLFPLFADLQGRRTLVVGGGAVAARKAQALLEAGAQVIVGAPE
                     LDAALSRLAADGRLTHLSGPFEPAWLDGVWLAIAATDDRAVNAAVHAAAQARQVFCNV
                     VDDAELSSFQVPSIVDRSPLIVAISSSGVAPVLARRLRERLESLFDHALGPLAELAAR
                     HRSRIRGALPDLGQRRRFYDWLLDGPVAGLLRRQQGDAAEAALRQALEQPQDRAAGSV
                     VLVGAGPGDPGLLTLKALRALNEADVILHDRLVSDEILALARRDAERIAVGKQLGENH
                     EATQARIHALMAEHARAGRRVVRLKGGDAFIFGRGGEELEYLREQGVPYEVVPGITAA
                     LACAAYAGIPLTHRGHAQSVRLVTAHCREDEEALDWDGLARGRQTLAFYMGVGQLALL
                     SQRLIAHGRPAGTPVALVENGSRPEQRVVTGALAELAALAERHAVHAPALLIVGEVAG
                     LARRLHWYGKLANPE"
     gene            7370..8047
                     /locus_tag="VGC69_04640"
     CDS             7370..8047
                     /locus_tag="VGC69_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294610.1"
                     /translation="MAGASARAAVCDAAFMHEGGVAQFVGQGPLTLSADLAFSEVAQQ
                     GAGQCRARVQGSASFVYMGIPTGQTRLDYLMTVKGGQASFVRYDKAGEAPSHNGQFDL
                     RMLGLFAYDQVREGQHLPGAAYHLNIGSDSPVGSQPGTTVRIGPKTVGARQTLSTPKG
                     SQACWPITYSRATDPTTLSLNGLVLPIPGVTSTVTDWYCPQLNLVVRQDIAQAEAKSS
                     VVLTQLR"
     gene            8171..8488
                     /locus_tag="VGC69_04645"
     CDS             8171..8488
                     /locus_tag="VGC69_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEY0294611.1"
                     /translation="MSATKKADDLKEKLIDNAKTSLDDAEELLREAASSTGDKAAELR
                     ERAMAALKRTRETLYDAQDAVLQKGRRAVRATDDYVHDKPWQAISIAAVGGLLVGMLI
                     CRR"
     gene            8508..8909
                     /locus_tag="VGC69_04650"
     CDS             8508..8909
                     /locus_tag="VGC69_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY0294612.1"
                     /translation="MSLRRSLRGVASSLLGLVRTRLELMSLEAAEEKSRVIALLGMAA
                     AALLFLALALLVLTAAIAVAFWPTEQRYIALACLGGLYALIGLVLLYKVRHELVTHPI
                     PFAATLEVLERDVAALEQGGSEAVAAAGRER"
BASE COUNT         1361 a         2863 c         3351 g         1471 t
ORIGIN      
        1 caggtgggcg aggcgggcag caggttgagc gccacggtgt agcgcgggtc gctcgggtcg
       61 gtgttgagcg cgtggtcgat ggcgtcgcgc gcctgcagct gctggtcggt attggcgtag
      121 cgggcgcgga tggtgccgag cgtgccgttc tgctcgtagt agatgctttc gggcggcagc
      181 ttggcgtcgg acaggatttg ctggatctga tccaccaggt ccgggtctac cttgacgacg
      241 gccttggcgc tggacacctg cacggcgggc gattcgccgt agaaattggg cagggtgtag
      301 agcaggccga tcacgaccgc gacgaggacc gtgatgtact tccagagggg atagcggttc
      361 attgccagca actgtgatga gcggcgcttc ggaccggatg gcgcagccgc cgcgggccgc
      421 ggcggccggc ttcccggccc gtcgggcctt gttcaggcct tacagggcct tgatggtgcc
      481 cttgggcagc acggtggtga cggcggtctt ctgcagcacg acctcgaccg gcttgtcggc
      541 cagttcggac acttcggcgg tgatgtagct gtcggtgacc ttggtgacct tgcccagcat
      601 gccgcccgag gtgacgattt cgtcgccctt ggacaaggtg gcgatcatgt tgcgggattc
      661 tttctggcgc ttcatctgcg gacggatcat caggaaatag aggatcacga acatcagcag
      721 gatgggcagc atgctcatga tggggttgcc ttcggcggca gcctggccca ggacgagcgt
      781 ggcggtattg aggacggaca ttgtctttga ctccggggtt tatctatatc tatctgtcgg
      841 tatggatgct gcgatctgtc ccccgcgcga gctttggaca gggcaagcca actattgtag
      901 cggggttgtc cgggttcttg agccgccgag gctgccctgc gcaattccga tgctcgcccc
      961 aggtggggct gcgcttcgga tgcctccggg gtcctcggct ggtgtccgag ccaggcggcg
     1021 gtcgctaatc tatgcctttg gcgcggtcct gggcgaattc ggcgcgccag gcatcgaaac
     1081 gcccttcggc gatggcttcg cgcatctgtt tcatcaggtc caggtagaag tgcagattgt
     1141 gcagggtatt gagccgcgcg ccggaaattt cgttggcgcg ctgcaggtgg tgcaggtagg
     1201 cccgcgagaa gttcaggcag gtgtggcagc ggcaggccgg gtccagcggg cgggtgtcgt
     1261 cgcggtagcg ggcgttgcgg atcttcaggt cgccgtggcg ggtgaacagc cagccgttgc
     1321 gggcgttgcg ggtgggcatg acgcagtcga acatgtcgat gccgcgcgcc acgccgtcca
     1381 ccaggtcttc gggggtgccc acgcccatca ggtagcgggg acggtcggca ggcagccgcg
     1441 gggccacgtg cgacaggatg cgcatcatgt cttgcttggg ctcgcccacc gacaggccgc
     1501 cgatggcgta gccgtggaag ccgatgtccg ccaggccggc gagcgattcg tcgcgcagcg
     1561 cctcgaacat gcccccctgc acgatgccga acagcgcatt ggggttgccc aggcggtcga
     1621 actcgtcctt cgagcggcgc gcccagcgca gcgacatgcg catcgagccg gcggcctcgt
     1681 cgagcgtggc cggccggccc ttgatttcgt agggcgtgca ctcgtcgaac accatggcga
     1741 tgtcggagtt cagcgacttc tggatgcgca tggactcctc gggcgtgaga aagaggcgcg
     1801 cgccgtcgat gggcgaggcg aacttcacgc cttcttcggt gatcttgcgc atgccctgca
     1861 ggctgaacac ctggaagccg cccgagtcgg tgagcagggg cttgtcccac tgcatgaagc
     1921 cgtgcaggcc gccgtgcttg tccagcacct cggtgccggg gcgcagccac aggtggaagg
     1981 tgttgcccag cacgatctgc gagccgatgt ccttcagctc gtggggcagc atgcccttga
     2041 cgctgccgta ggtgcccacg ggcatgaaga tgggcgtctc gaccacgccg tggttgagcg
     2101 tcatgcggcc gcggcgcgcg gcgccgtcgg tggccaggag ttcgaagttc agtccggtca
     2161 tgggcggggg ggtgttcctg cggattcgat gaacatcgcg tcgccatagc tgaagaagcg
     2221 atagcgctgt tcgaccgcgt gcgcataggc gcggcggatg ggctccaccc cggccagcgc
     2281 cgacaccagc atgagcaggg tggattgcgg caggtggaag ttggtgacca gggcgtcgac
     2341 cacctggtat gggtagcccg gggtgatgaa gagccgcgtg tcgccttgcg cggcggccag
     2401 gggctggccg gcggcggtgg cgcaggccgc ggcggccgat tcgagggcgc gcacgctggt
     2461 ggtgcccacg gcgatcaccc ggccgccgcg cgcgcgcgtc tgcgcgatgg cgtcgaccgt
     2521 ggcctgcggc acggtgtacc actcggcgtg catgatgtgg tcggccaggt tggccgcgcg
     2581 cacgggctgg aaggtcccgg cgccgacgtg cagggtaacg aaggcgcgcc gcgcgccctg
     2641 ggcggcgagc tggtcgagca tggcctggtc gaagtgcagg cccgcggtgg gcgcggctac
     2701 cgcgccgggc tcgcgggcgt agaccgtctg gtagcggctg tcgtcgtcgg cttgcgcgtc
     2761 atgcgtgatg tagggcggca ggggcgtggc gccgtgggcg tcgagcaggt cgagcaccgg
     2821 accgggaaag cgcaggtcga agagttcgcc ctggcggccg agcacctcgg tgtcgaaggc
     2881 gtcggccagg cgcaggatca tgccggggcc gggggatttg ctggcgcgca cgtgggccag
     2941 cgcgcgggtg ggttcggtga tgcgttcgac cagcacttcg actttgccgc cggtggcttt
     3001 ctggccgagc aggcgggcct tgatgacgcg ggtgtcgttg aagacgagga ggtcgttttc
     3061 tcggaccagg ccggcgaggt cggggaattg ccggtcatgc agccggcccg cgccgtcgag
     3121 gtgcagcagg cggctgccgc ggcgctcggc cgcgggatgc tgggcaatga gctcgtcggg
     3181 cagggggtag tcgaagtcgg ccagggtgaa ggccggggag gggaatgcgg gcacgttgtg
     3241 gggagatggg ggcgaaccgc ttattttaag ctgcatttgg ttatgcttct cgagcttgat
     3301 ggtttgagtc cttgagacgc tgccgccgcg ggccagccgg gcgcggcccg atcggcgcgg
     3361 cccgatcggc ggggcccgat cggcggggcc cgatcggcgg ggctcgggcg cgccatcagg
     3421 cgccctcggc ccctgcgggg ctgcccccgc ctcgaagggc cgcgcccggc tggcccgcgg
     3481 cggcagctca tgacatcatc agcgcgttcg tcgaaaagta tcttctttta tgcacatatc
     3541 cggattcaaa acatggctgg ccgcagttgc cgcggcctta tgcgtggggc aggtcggggc
     3601 gcaggctttg ccggccaatc ttgccgcggc ctggcagcag acgggattgc ctgaaaacgc
     3661 catgtcgctg gtggtgcagg aagtgggcgg gccgcggctg gcggcggtga acgcgggcgt
     3721 ggcgcgcaat ccggcctcgg tgatgaagct ggtgacgacc tgggcggcgc tgtcggagct
     3781 ggggcccacg tatgtatggc agaccgaact ggtgagcgaa cccggggcgg cggtcagcaa
     3841 cggcgtgctg tcgggtccgc tctatgtgcg ggcgtcgggc gatccgcagc tgatggtgca
     3901 ggacctctgg acgctgctgc gcgacctgcg gctgcggggc gtgcggcagc tcggcgacgt
     3961 ggtggtggac cgcagcctgt tcggcaatgt ggcgaccgac ccgggcgctt tcgacaatgc
     4021 gccggaccgg gcctacaacg ccagtccgga tgccttcatg gtgggtttcg gcgcggagcg
     4081 cctgatgttc atggcggatg cgccggcccg cgcgtggcgg gtggtgatgg atccggccct
     4141 gcccaatgtg aagctggagg gcaggctggc ctggagcgat gcgccctgcc aggggtcgcc
     4201 cgaggtcaac accttgccgg tggcgacgcc gcagggcacg accctgcggt tgtcgggcac
     4261 catgcccggc gcctgcggcg atttcagcct gtaccggctg gccctgacgc agcccggcca
     4321 tgcggccggg gtgttccggc agatatggca ggaactgggc ggcacgatga cggggcagct
     4381 gcgcgacggc ctggcgccgg tggacggcga ggtgctggcc agccatgact cgccggagct
     4441 gggcgagacc ctgcgcacca tcaacaagcg cagcaacaat gtgatggcgc gcacgctgct
     4501 gctgacgctg ggcgcggagc gcggccggcg gcccgccacg gtggccagca gcgatgacgt
     4561 ggccaggcgc gtgctggccg ggcagggctt gctgatgccc gagctggtgc tcgacaacgg
     4621 cgcgggcctg gcgcgcgagg cgcgggtgtc ggccgacagc ctggccgcgc tgctgcgcac
     4681 ggcctggagt tcgccctata tgccggaata tgtggcgtcg ctggccatca ttggcgaaga
     4741 tggcacggtg aggaagcgcc tgcgcgacgg cgcggcggcg ggccgcgcgc atctcaagac
     4801 cgggtcgctg cgcgatgtgc gcgccatcgc cggttatgtg acgggcgtca gcggcaagcg
     4861 ctatatcgtg gtgtgcatcg tcaaccacga gcaggcggcc gcggtacgat ccttcgacga
     4921 cgcgctcgtc gattggctgg ccaggcggtg acggcgttgt gcatccgttc gcgatgcgcg
     4981 ccatcaatat aaatagagag aacacgcacc atgcccgtac atgaaatccg ccatccgctg
     5041 atccgccaca agctgggcct catgcgccgc gccgacctca gcaccaaggg ctttcgcgaa
     5101 ctggccggcg agctcggcgc gctgctgacc tacgaggcgt ccaaggactt gccgctctgc
     5161 cccgccgagg tcgaaggctg gaacggcacg gtgcaggtgg aaaagctggc gggcaagaag
     5221 atcacggtgg tgccgatcct gcgcgccggc ctgggcatgc tcgacggcgt gttgagcctg
     5281 atcccgggcg ccaaggtgag cgcggtgggc ctggcccgca acgaggcaac gctgcaggcc
     5341 cacacctacc tggaaaaact ggtgggcgaa ctcgaccagc gcatcgcgct catcgtcgat
     5401 cccatgctgg ccacaggcgg ttcgatgctg gccaccgtcg acctgctcaa gcgcgccggc
     5461 gccagggatg tccgggcgct ggtgctggtg gccgtgcccg aaggcatcag gaacgtgctc
     5521 gacaagcatc ccgacgtgca gatctacacg gcttgcatcg accagggcct gaacgagcat
     5581 ggctacatcc tgccgggcct gggcgacgcg ggcgaccgca tcttcggcac caagcagaag
     5641 gccgattaag acgcggcgcg gccgccgcgg gaatcattcg gggttggcca gcttgccgta
     5701 ccagtgcagg cgccgcgcca ggcccgccac ttcgcccacg atgagcagtg cgggcgcgtg
     5761 cacggcgtgg cgttcggcca gggcggcgag ttcggccagg gcgcccgtca cgacgcgctg
     5821 ctcgggccgg ctgccgtttt ccaccagggc caccggcgtg ccggctggcc ggccgtgcgc
     5881 gatgagccgt tgcgacagca aggccaattg ccccacgccc atatagaagg ccagcgtctg
     5941 cctgccgcgc gccaggccgt cccagtccag cgcctcctcg tcttcgcggc aatgcgcggt
     6001 gaccaggcgc accgattggg cgtggccgcg gtgcgtcagc gggatgccgg cgtaggcggc
     6061 gcaggccagc gccgcggtga tgccgggcac cacttcatag ggcacgcctt gctcgcgcag
     6121 gtattccagt tcttcgccgc cgcggccgaa gatgaaggca tcgccgcctt tcaggcgcac
     6181 cacccgccgg ccggcgcggg cgtgttcggc catgagggca tggatgcggg cctgggtggc
     6241 ctcgtggttc tcgcccaact gcttgcccac ggcgatgcgt tcggcatcgc ggcgcgccag
     6301 ggccaggatc tcgtcgctga ccaggcggtc gtgcaggatc acgtcggctt cgttcagggc
     6361 gcgcagggcc ttcagcgtca gcaggcccgg atcgccggga cccgcgccca ccagcacgac
     6421 gctgcccgcc gccctgtctt gcggttgttc gagcgcctgg cgcagtgcgg cctcggccgc
     6481 gtcgccctgt tggcggcgca gcaggccggc cacggggccg tcgagcagcc agtcgtagaa
     6541 gcggcggcgc tggcccaggt cgggcagggc gccgcggatg cgcgagcgat ggcgggcggc
     6601 cagttcggcc agcggcccca gcgcatggtc gaacagcgac tccaggcgtt cgcgcaggcg
     6661 gcgcgccagc acgggcgcca cgccggaaga cgagatcgcc acgatcagcg gcgaacggtc
     6721 gacgatggac ggcacctgga aagaagacag ttcggcgtcg tcgaccacat tgcaaaatac
     6781 ttggcgggcc tgggcggccg cgtgcacggc ggcgttgacg gcgcggtcgt cggtggcggc
     6841 gatggccagc cagacgccgt ccagccaggc gggttcgaaa ggtccggaca gatgcgtcag
     6901 gcgtccatcg gccgccagcc gggagagggc agcgtcgagt tcgggggcgc cgacgatgac
     6961 ctgggcgccg gcttccagca aggcctgggc cttgcgggcg gcaacggcgc cgccgccgac
     7021 gaccagggtg cgccggccct gcaggtcggc gaagaggggg aaaagcttca tggagaattg
     7081 ctgcacggac cgggcagcaa aaagctgccg cgagaaggat aggggccgcg caggcgtcct
     7141 acaacgtaga atttgttgaa tggatatgcg caacagtcat atgcgcgacg ccccattgcg
     7201 ccgttcagca atattgctga tgactaccga accaccgggg agcaagcccc gcgccggttc
     7261 gcccagtgta gtatggaacg gcctgcagca ttaataggtg atttctcggg agcggtccgt
     7321 ggcggtacgt ggcaagtgga tttcctggtt gattcttccg gcggcgctga tggcgggcgc
     7381 ctcggcccgg gcggccgtct gcgacgccgc cttcatgcac gagggcggcg tggcccagtt
     7441 tgtcgggcag gggccgctta ccctgtccgc cgacctggcg ttctccgagg tggcccagca
     7501 gggcgccggc caatgccgcg cccgcgtgca ggggtcggcc agcttcgtct acatgggcat
     7561 ccccaccggg cagacccggc tcgattacct gatgacggtc aagggcggcc aggcctcgtt
     7621 cgtacgctac gacaaggcgg gcgaggcgcc gtcgcacaat ggccagttcg acctgcgcat
     7681 gctgggcctg ttcgcctacg accaggtccg cgagggccag cacctgccgg gagcggccta
     7741 ccacctgaac atcggatccg actcccccgt gggcagccag cccggcacca ccgtgcgcat
     7801 cggcccgaaa accgtcggcg cgcgccagac cctgagcacg cccaagggca gccaggcttg
     7861 ctggcccatc acttattcgc gcgccaccga tcccaccacg ctgtcgctca acggcctggt
     7921 gctgccgatt ccaggcgtga ccagcacggt gaccgactgg tattgcccgc aactcaacct
     7981 ggtggttcgc caggacatcg cgcaggccga ggccaagtcc agcgtagtgc ttacgcaatt
     8041 gcgctgatcc tggcggccgg ccgagacggc gctgtagcaa gccgtagcaa ttcaatccgg
     8101 ccccccctct ggtggctggg tagcatggcc ctatatatag cctgggtact ttctttttaa
     8161 ggagtctttc atgtccgcga ccaaaaaagc cgatgatctc aaggaaaagc tgatcgataa
     8221 cgccaagacc agcctggacg acgccgagga attgctgcgc gaagccgcca gcagcaccgg
     8281 agacaaggcg gccgagctgc gcgagcgcgc catggctgcc ctcaagcgca cccgtgaaac
     8341 gctctacgac gcgcaggacg cggtcctcca gaaaggccgc cgcgccgtcc gcgccaccga
     8401 cgattacgtg cacgacaaac cctggcaggc gatttcgatt gcggccgtgg gcggcctgct
     8461 ggtgggcatg ttgatctgcc gccgttaaga ccgccgcgac cgccgccatg agtctgcgcc
     8521 gatcccttag gggcgtggca tccagcctgc tggggctggt gcgcacgcgg ctggagctga
     8581 tgtcgctcga ggccgccgaa gaaaaatccc gcgtgatcgc cctgctgggc atggcggcgg
     8641 cggccttgct gttcctggcg ctggccctgc tggtgctgac ggcggccatc gcggtggcat
     8701 tctggccgac cgagcagcgc tatatcgccc tggcctgcct gggcggcctg tacgccctga
     8761 tcggcctggt cctgctctat aaggtaaggc acgagctggt cacgcacccc atcccttttg
     8821 ccgccacgct cgaagtgctg gagcgcgatg tggcggcgct ggagcagggc ggcagcgagg
     8881 ctgtcgcggc cgctggacgg gagcgctgag atgagccgtc cgacctccac cgtcgaccgc
     8941 caggtgcgca tcgaactgct gcgcacccgc gccgccgtcg agcgcgaagc cctggccaag
     9001 caactggccg aagccagcca ggccctgggc cctggtcaat tattgc
//
LOCUS       DASWPO010000079         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2159676, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000079 DASWPO010000000
VERSION     DASWPO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159676"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VGC69_04655"
     CDS             complement(<1..936)
                     /locus_tag="VGC69_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY0294613.1"
                     /translation="MNTQYRKPLPGTPLDYFDAREAVEAISPGAYEPLPYTSRVLAEN
                     LVRRCEPALLRAALGQLIERKRDLDFPWFPTRVVCHDILGQTALVDLAGLRDAIADQG
                     GDPAQVNPVVPVQLIVDHSLAVECGGFDPDAFAKNRAVEDRRNEDRFHFIDWTKLAFR
                     NVEVVPPGNGIMHQINLERMSPVIHAQNGVAYPDTLVGTDSHTPHVDALGVIAVGVGG
                     LEAENVMLGRASWMRLPDIVGVELTGRPQPGITATDVVLTLTEFLRREKVVGAYLEFY
                     GEGASSLTLGDRATISNMAPEYGATAAMFAIDPQTI"
     gene            complement(936..1646)
                     /locus_tag="VGC69_04660"
     CDS             complement(936..1646)
                     /locus_tag="VGC69_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0294614.1"
                     /translation="MSKILKLPVPPAAEGETLSDHAFRQIQSAIVKGEIPPGSKISEP
                     ELVRTYGVSRGPLREALHRLEGQRLLVRVPHAGARVVSLSRQELSELYQIRESLEGMA
                     CRLAAEHMTPQAIAELRRVLEAHERDEAFQAGRGYYLQEGDYDFHYRIVQGSGNQMLF
                     RLLCDELYQLARMYRIQYSNSPNRPRQAYTEHHRILDAIAAGDGELAELLMRRHIRAS
                     RLNIEHEIAQAGLQRTEA"
     gene            1838..2266
                     /locus_tag="VGC69_04665"
     CDS             1838..2266
                     /locus_tag="VGC69_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0294615.1"
                     /translation="MLMLWVKALHIVFIASWFAGLFYLPRLYVNLAQQTDAGAQALLL
                     GMARRLYRFTTMLAFPAVLLGLWLFVGYGIGMGPGNGWMHAKLLLVVLVIGYHHACGV
                     MLRKFEQGRNTRSHTFYRWFNEIPIVLLLIVVVLVVVKPF"
     gene            2277..>3349
                     /locus_tag="VGC69_04670"
     CDS             2277..>3349
                     /locus_tag="VGC69_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY0294616.1"
                     /translation="MPSDDTGRPRKTLTLARPARTEPDDDASRRKRSGARAHNAVQQA
                     YAREKAERRQSPDLSRGHRERLSGQLEEGKPAGPRAPDGAPEPDDLAGQALTVPAAAQ
                     DPDDVPLIEKRGQRLSARQAEVFHVFAPCPQGLEEALTAEMQALGFEDAQPGRAGCRF
                     TADWSGIQRANLYSRLATRILAQVAQDEISHEDDILDLAYSVPWERWFGAEQTLRVDT
                     SAIKSPVRSLQYCNLRAKDGICDRLRDREGARPDIDTVRPDARVHLFLSGTLATLYLD
                     TSGESLFKRGWRLDKGEAPLRENLAAGLLALAGWDPTAPLLDPFCGSGTILIEAAWIA
                     LGVPAGISRPFGFERLRGFDYRH"
BASE COUNT          542 a         1166 c         1085 g          556 t
ORIGIN      
        1 gatggtctgc ggatcgatgg cgaacatggc cgcggtggcg ccgtattccg gcgccatgtt
       61 ggagatggtg gcccggtcgc ccagcgtcag gctcgacgcg ccctcgccgt agaactcgag
      121 gtaggcgccc accacttttt cgcggcgcaa gaattcggtg agcgtcagca cgacgtcggt
      181 cgcggtgatg ccgggttggg ggcggccggt gagctcgacg cccacgatgt cgggcagccg
      241 catccaggag gcgcggccga gcatgacgtt ctcggcctcc agcccgccca cgccgacggc
      301 gatcacgccc aatgcgtcga catgcggcgt atggctgtcg gtgccgacca gcgtgtcggg
      361 ataggccacg ccgttctgcg cgtggatgac cggcgacatc cgctcgaggt tgatctggtg
      421 catgatgccg ttgcccggcg gcaccacctc gacgttgcga aaggccagct tggtccagtc
      481 gatgaagtgg aagcggtctt cgttgcggcg atcttccacc gcgcggttct tggcgaaggc
      541 gtccgggtcg aagccgccgc attcgaccgc cagcgaatgg tcgacgatca attgcaccgg
      601 cacgaccggg ttgacctgcg ccgggtcgcc gccctggtcg gcgatggcgt cgcgcaggcc
      661 ggccagatcc accagggccg tctggccgag gatgtcgtgg cacaccacgc gggtcgggaa
      721 ccagggaaag tcgagatcgc gcttgcgctc gatgagctgg ccgagcgcgg cgcgcagcag
      781 cgcgggttcg cagcggcgca ccaggttttc ggccagcacg cgcgaggtgt acggcagggg
      841 ctcgtaagcg ccgggagaga tcgcctccac ggcctcgcgc gcgtcgaaat agtccagcgg
      901 ggtgcccggc aagggcttgc ggtattgagt gttcatcatg cctcggtacg ctgcaggccg
      961 gcctgcgcga tttcgtgttc gatgttgaga cgggatgcgc ggatatgccg gcgcatcagt
     1021 aactcggcga gctcgccgtc gcccgcagcg atggcgtcga gtatgcggtg gtgttccgta
     1081 taggcctggc gcggccggtt gggcgagttc gagtactgga tgcggtacat gcgcgccagc
     1141 tggtagagct cgtcgcacag caagcggaac agcatctggt tgccgctgcc ctgcacgatg
     1201 cgatagtgga aatcgtaatc gccctcctgc aggtaatagc cgcgcccggc ctggaaggcc
     1261 tcgtcgcgct cgtgcgcctc caacacccgg cgcaactcgg caatggcttg cggcgtcatg
     1321 tgttcggcgg ccagccggca ggccatgccc tcgagcgatt cgcggatctg gtagagctcg
     1381 ctcagttcct ggcggctgag cgagacgacg cgcgcgccgg catgcggcac gcgcaccagc
     1441 aaccgctggc cctccagcct gtgcagcgcc tcgcgcaatg gtccgcggct gacgccgtag
     1501 gtgcgtacca gctcgggctc ggaaatcttg ctgcccggcg ggatctcacc cttgacgatg
     1561 gccgactgga tctggcgaaa cgcgtggtcc gagagtgtct cgccctcggc tgccggggga
     1621 accggcaatt tgagtatctt gctcattttg tcgacgatct tctgattttt atgaaaaaac
     1681 aatagcaaaa tccgagtttc tttgcaagat tgtcgacaaa acaagccaat ccacgcaata
     1741 tcggcgctca ccgcaggcca gcgccgaacc gatggcctgg ccgggcatcc cagagcgccc
     1801 gcttacaatg cggggattct tccttacggg cacaaccatg ctcatgctct gggtcaaggc
     1861 tctgcacatc gtcttcatcg cctcgtggtt tgccgggctg ttctacctgc cccgcctcta
     1921 cgtcaacctg gcccagcaga ccgacgccgg cgcgcaggcc ctgctgctgg gcatggcccg
     1981 ccgcctctac cgcttcacca ccatgctggc ctttcccgcc gtgctgctgg gcctgtggct
     2041 gttcgtcggc tacggcatcg gcatggggcc gggcaacggc tggatgcacg ccaagctgct
     2101 gttggtggtg ctggtcatcg gctaccatca tgcctgcggc gtcatgctgc gcaaattcga
     2161 gcagggccgc aacacccgct cccacacgtt ctatcgctgg ttcaacgaaa tccccatcgt
     2221 gctgcttctc atcgtggtgg tgctggtggt cgtcaaacca ttctgaatcc ctaagcatgc
     2281 catccgacga taccggccgt ccgcgcaaga cgctgacact ggcccgcccc gcacgcaccg
     2341 aaccagacga cgacgcctcg cgccgcaagc gcagcggcgc acgcgcgcac aatgccgtgc
     2401 aacaggccta cgcccgcgag aaagccgaac gcaggcagtc cccggacctg agccgcgggc
     2461 accgtgagcg cctgtccggc caattggagg aggggaagcc cgcagggccg agggcgcctg
     2521 atggcgcgcc cgagcccgac gacttggccg gacaggcgct cacggtgccc gcggcggctc
     2581 aagatcccga tgacgtcccg ctaatcgaaa agcgcggcca gcgcctgagc gccaggcaag
     2641 ccgaagtctt ccacgtcttc gccccctgcc cccaaggcct ggaagaagcc ctgaccgccg
     2701 aaatgcaggc cctgggcttc gaagacgccc agcccggccg cgccggctgc cgcttcaccg
     2761 ccgactggag cggcatccag cgcgccaacc tgtattcgcg cctggccacc cgcatcctcg
     2821 cccaggtcgc ccaggacgaa atcagccacg aagacgacat cctcgacctg gcctacagcg
     2881 tgccctggga acgctggttc ggcgccgaac agaccctgcg cgtcgatacc tccgccatca
     2941 agagccccgt gcgcagcctg cagtactgca acctgcgcgc caaggacggc atctgcgacc
     3001 gcctgcgcga ccgcgaaggc gcccggcccg acatcgacac cgtgcgcccc gatgcccggg
     3061 tgcacctgtt cctgtcgggc accctcgcca ccctctacct ggacacctcg ggcgaatcgc
     3121 tcttcaagcg cggctggcgc ctggacaagg gcgaggcccc cttgcgcgaa aacctggccg
     3181 ccggcctgct ggccctggcc ggctgggacc cgaccgcccc cctgctcgac cccttctgcg
     3241 gcagcggcac catcctgatc gaggccgcct ggatcgccct gggcgtgccg gccggcatct
     3301 cccgcccctt cggcttcgag cgcctgcgcg gcttcgacta ccgccattg
//
LOCUS       DASWPO010000080        26656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3053847, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000080 DASWPO010000000
VERSION     DASWPO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 26656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26656
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053847"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGC69_04675"
     CDS             <1..195
                     /locus_tag="VGC69_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEY0294617.1"
                     /translation="LKSEFPAITELRSAVLWWKGHSKVVPDYYVDKLPTNPWIHQPFE
                     DYDSLRPHQLEAWIRKGLKG"
     gene            375..587
                     /gene="rpsU"
                     /locus_tag="VGC69_04680"
     CDS             375..587
                     /gene="rpsU"
                     /locus_tag="VGC69_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY0294618.1"
                     /translation="MPIVRLKENEPFEAALRRFKRTIEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRHYKRIRSQQLPPRMY"
     gene            701..2716
                     /gene="dnaG"
                     /locus_tag="VGC69_04685"
     CDS             701..2716
                     /gene="dnaG"
                     /locus_tag="VGC69_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY0294619.1"
                     /translation="MIPESFIQDLLARVDVVDVVGRYVQLRKGGANLLGLCPFHNEKS
                     PSFTVSPTKQFYHCFGCGAHGTAITFLMEHTGSNFPEAVRTLAGQVGMTVPEEPRSPR
                     QQAETVRRKAEVSRHTQVLDQAQVYYLKQLRASPAAIAYLKQRGLTGEIAAHFGLGWS
                     GTDRHGLSKVFDDYADPVLVESGLVIESEDGRRYDRFRERVMFPIRNARGSLIGFGGR
                     IIGDGKPKYLNSPETPIFSKGQELYGLWEARQAIREEGMVIVVEGYMDVVGLAQLGIA
                     NAVAALGTATTPDHVKKLLRASDRVVFSFDGDAAGRRAAWRALQACLPVLRDDINIRF
                     LFLPAEHDPDSYVREFGAEAFRAVLGEASALSRFLLDELASHHDLGEAEGRASCLHEA
                     APLLAAIPDCALKVQIERELARQVQLTPEEMTQVLVQVQARQAPRGPLPVPGQEAAPA
                     PPHQAGRTDAGPGAAPPAWESNDLPADVGYEPEPWQGEGGWSGQPTGGRRNGGGFSSQ
                     NGQNRPWRGRRDERPTGFVGRRPMPSMAKRLLCLLLANPGLVDGMGDQQLEVIDHDPQ
                     LGLVRDLIMLAQASGARHLGALLQAAEPDSDLSVVLQGLRAEVLVMEDLPDPQGEWNY
                     ALRRIEHSWVKAEMARLAEEGLQTDESRQRFQELGGRMTILRNAGIR"
     gene            2828..5167
                     /gene="rpoD"
                     /locus_tag="VGC69_04690"
     CDS             2828..5167
                     /gene="rpoD"
                     /locus_tag="VGC69_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY0294620.1"
                     /translation="MSKSTGKTTSAIDAEKTKASKAVGKAAPPAAVKKADAKPAATKV
                     AAKPAAKAATAGATAVKTAGKPAAKAAAKSAVAKKAAPAAADKAPRAKKASAAAEDKL
                     ADLVAAPKVGGTGRRPGRPAKNANPDAEGFEDSMDGEGEVLPELKPVKRGGKRGKGDA
                     KELLSRGPLTPEEHEARRNRLKQLIKLGKDRGYLTYGEINDHLPDDLVDAEAIDGIIS
                     TFSDMGIAVYDQAPDADTLLMSENAPVASNDDDVEDEAEAALTTVDSDFGRTTDPVRM
                     YMREMGSVELLTREGEIEIAKRIEDGLKHMVMAISACPTTINEIISHATQVREGQKQI
                     DEVVDGLVDPEDGEEYAGAGVTADEDENDEGPAGGMSSKQLEDLRVKSLEKFDTVVRQ
                     FDKMRLSYEKEGYKSDAYTKAQESIQNELMGIRFTAKMVEKLADTLRNQVEEVRQLER
                     AVLHICVDRAGMPRPHFLKAFPGNETNLQWILDEVAAKPPYAETLERQIPAVQELQQK
                     LIDLQTRVVLPLKDLKDVNKRMATGEAKARKAKREMTEANLRLVISIAKKYTNRGLQF
                     LDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIET
                     INKMNRISRQILQETGAEPDPATLAQKMDMPEDKIRKILKIAKEPISMETPIGDDDDS
                     HLGDFIEDNSTLAPSDAALHGSMRDVVKEVLDSLTPREAKVLRMRFGIEMSTDQTLEE
                     VGKQFDVTRERIRQIEAKALRKLRHPSRADKLKSFLEGQ"
     gene            5220..5298
                     /locus_tag="VGC69_04695"
     tRNA            5220..5298
                     /locus_tag="VGC69_04695"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5256..5258,aa:Ile,seq:cat)
     gene            5563..5958
                     /locus_tag="VGC69_04700"
     CDS             5563..5958
                     /locus_tag="VGC69_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294621.1"
                     /translation="MALISCPECARQISDKAAACPSCGCPISVSEKMQQSTAAMNAPV
                     QTVTLSKSRGVYIILGLLFGYLGFHNFYAGQHGRGAIKLILLLVTVVLDGATGFHTGF
                     SLIVIVLTTIWSLLEILFVTRDAAGNVFS"
     gene            complement(5980..6399)
                     /locus_tag="VGC69_04705"
     CDS             complement(5980..6399)
                     /locus_tag="VGC69_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294622.1"
                     /translation="MSDALCQLESNKGTWFVTTPGTVTIHRSYNPIHLKCTKDGVDPG
                     IATVESSTKGMAWGNILAGGIIGAAVDMGTGSAYDYPPLITVLMGQTITVVPPKPNND
                     KDVPAKPEDAQQASTQATTTQAAPAPTPIQIGPTSPQ"
     gene            complement(6646..8115)
                     /locus_tag="VGC69_04710"
     CDS             complement(6646..8115)
                     /locus_tag="VGC69_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294623.1"
                     /translation="MELFSDLPGDEGLPGRKRRLAMIAVMTSTTMAVFDGSIVNIALP
                     QIARALDVPAGAAIWVSNGYLLSVAVALATFAALAKRLGFRALFAGGLAVFTLASLGC
                     ALSSSLGWLTAMRVLQGIGGAATLSIAPAIHRSVFPNRLLGRILGLHALLIAASTAIA
                     PILGGTLLSVLSWQWIFAINVPLGIVGVTLALRTIPGEHAPTDKPFDIAGALLSAIAM
                     AALILTADACSRLGAGERTLVSVLTPIAYGLTAAAAGAAFVWRQRRAQDPLLPLDMFS
                     SSRFSMAALTSLASFVGQGITFIALPILFENVYDHSAFVTALLFTPWPIGIILAAPHA
                     GRLADRHQPAALATMGLSIFVIGLALLALLPRHANAWDISWRELLCGAGFGIFQSPNN
                     REMMANASRERSGNASGVLAIVRTFGQCLGTTLVGVILSVYAPQAITGADGLMSITQS
                     AQAIQLALWLAAAATVLGLVISASRLRGASARLRREESE"
     gene            8310..9239
                     /locus_tag="VGC69_04715"
     CDS             8310..9239
                     /locus_tag="VGC69_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294624.1"
                     /translation="MPEPDLNLLTALDALLADGSVAGAARRLGLSASAMSRTLARLRE
                     TLGDPLLVRAGRGMVLTPHAETLRQRTRNAMHEARALLRPSTAEPDFSTLQRTFTIRA
                     NDGFVEVLGAPLIVAAMAIAPLVRLRFAPKPEKTAVHLREGSADLEIGVLGEMGPEVR
                     VQALFRDRFLGVVRRGHPLELEHEITAERYVAFGHVVASRRGRTNGPVDEALAALGLE
                     RTVVAMVPSFPAALAVARASDLVALVPASFLGNQPGHRDSASSSTTYAFELPVTTEKI
                     TISQMWHPRLEADPVHRWLRQLVLAVCRERVPL"
     gene            complement(9252..10016)
                     /locus_tag="VGC69_04720"
     CDS             complement(9252..10016)
                     /locus_tag="VGC69_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294625.1"
                     /translation="MSVSPSLPVAWIAGVGAAAGLGGALAHRFAQAGYAVALTGRTAA
                     RLEALADQIRAAGGQALALPASVADEAGLARAAAQVRELGPLHLGLFNAAEGARAPSL
                     DLSAAQFEQAWRTSTLGGFLFAQAVLRALLDNGVDPASPTGRGSLLFTGATAALRGRP
                     PFAAFAAAKAALRSLSQSLAREFGPQGIHVAHVVIDGGIDGERLRTNAPQRVQQAGDD
                     GLLQLQALADTYWQLHQQHRSAWTQELDLRPYKEPF"
     gene            complement(10045..10905)
                     /locus_tag="VGC69_04725"
     CDS             complement(10045..10905)
                     /locus_tag="VGC69_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0294626.1"
                     /translation="MTNANAALAGTFLIGNDFEIHRLGFGAMQITGPGIWGQPLDRAE
                     AIRTLKRLPDLGVDFIDTADSYGPDVSEPLIREALHPYTGLRIATKAGLTREGPDQWS
                     PKGDPAYLIQQAHKSLRQLGVEQIDLWQLHRIDPRVPRDEQFGAVKSLLDDGIIRQAG
                     LSEVSVADIEAASKVFKVATVQNRFNLIDREHEAVLDYCTKHQIGFIPWFPLASGDLA
                     KPGNALGEIAKRYKASPSQIALAWLLRRSPVMLPIPGTSKVSHLEANIAAAGLELSDD
                     DFAELDRAGS"
     gene            complement(10965..13274)
                     /locus_tag="VGC69_04730"
     CDS             complement(10965..13274)
                     /locus_tag="VGC69_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0294627.1"
                     /translation="MNPTRYTASHWGLYEVRHDAQGNPALAPWAQDPDPSPIGLHMLA
                     ANNAPQRILRPAIRAGWLENQRASRTARGTEPFVEVSWHDALDLVAEELRRVIDTHGN
                     QAIFGGSYGWASAGRFHHSVGHLHRFLNVLGGYVRSVDSYSYGAGGVLMPRIVAPIDA
                     ILSNSDDWETLARHTRLLVCFGGMPAKSAQSDSGGAGRHRLRDAMRHLARQGVRIVNI
                     GPSRNDAEPGAQAQWLPIRPNTDTALMLALAHGVLAEGRHDRAFLASHCVGWARFEAY
                     LLGQADGIAKDAVWASRITGAPREQIAELAHALGRERTLVTAMWSLQRAHHGEQPFWA
                     LTALAAVLGQIGLPGGGFAFGYGSTNSIGHRSARFQGPTLPQGRNAVDAFIPVARIAD
                     MLARPGEAFDYNGRSHRYPDIRLVYWAGGNPFHHHQDLNRLATLWRKPDTVIAHEQFW
                     NAHAKMADIVLPATTTLERDDIGFSSREAHLVAMRRVLPPAGEARDDFEIFRALASRL
                     GAQAPYDEGRDAHAWLRALYDETLPRAQAAGVELPGFDGFWEQGLVDFEGTTPPTPLL
                     RRFREDPGAHPLKTPSGKIEIWSETIASFGYADCPGHPSWLEPAEWLGAADAQQLHLI
                     SDQPATKLHSQLDHSALSQGVRRQGREPLTMHPEDARERGIQDGEAVRVWSPRGACIA
                     AAQLDDAVMRGVVKLATGAWFDPSGETPLLDKHGNPNALTMDLPASRLSQGCAAQTCL
                     VRVARWTGPLPEITAFEPPRFVAREARRA"
     gene            13463..14221
                     /locus_tag="VGC69_04735"
     CDS             13463..14221
                     /locus_tag="VGC69_04735"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0294628.1"
                     /translation="MQAILQGKTAIVTGAAGDLGAAIACCFLEAGANVAWLDRDAERA
                     SARAQAGPAPERSLAVHCDIASASSTQDAVARVAERFGGIDILVNNAATVSARKPLAE
                     LDIDAWRHTLDVNLNGAFLMSKWVIPHLLRKRAGVILNVASQLGHVTNPGGSAYSTSK
                     AALIGLTRSIAVDYAKDNIRAVSISPGSVMTSRLTDRYGSEAEVNAVLAPRHPLGRIA
                     QVAEVAQAALFLASDSASFITGTDLLVDGGYTAV"
     gene            14482..15378
                     /locus_tag="VGC69_04740"
     CDS             14482..15378
                     /locus_tag="VGC69_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0294629.1"
                     /translation="MKLSRIFAGVLLSTGFVGGAYAAGTCPIKEPVRFGVEPYESAQR
                     MIPVYNDVAKMIGQKIGCQVQLFIATSYNAEIEAMRNKKLEFAQFGPLGYVLAHQVAK
                     AEAVSSFVGPDGQPASYYASIVTWPGAGIDTLADVKGRSFAYSDPASTSGHLFPAYGL
                     SKNGIDPDKGVKAVYAGSHTASFEALRNHKVEAGELNSEEIASAKLHNEYDDSAYTTL
                     WKSDNIPLDPMTIRGDLPAGFKTRLAAVLSSLDFHELPDADQKFLAANGTPQLKTVPQ
                     NDAAYNQIRDLISTLHIDLKKL"
     gene            15375..16226
                     /locus_tag="VGC69_04745"
     CDS             15375..16226
                     /locus_tag="VGC69_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEY0294630.1"
                     /translation="MSFVQATRADMALDGEASLAFAGAASAAASPAAEAGSSVRLAVR
                     DLSMRYADGHLALNQLNLSVNAGEMVVVLGSNGCGKSTFMKCVVGLNRPTGGTVEVAG
                     RDLASLSGKALRAARLPIALISQNANLVRRRSVLDNVCAGSLGRHRTLATSLGRIPRG
                     EIEPARRYLDEVGLLHLAAQRAGTLSGGQAQRVAVARALAQRPHVLLADEPVASLDPE
                     AADEVMRLLRRLATDDGLAVVCVLHQPELAMRYADRLVGLRRGQLQFDQPAGQVSMQQ
                     VSSLYVS"
     gene            16237..17040
                     /gene="phnE"
                     /locus_tag="VGC69_04750"
     CDS             16237..17040
                     /gene="phnE"
                     /locus_tag="VGC69_04750"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY0294631.1"
                     /translation="MTPVLQNREAQPAVPLMRTLSSIGIAVVLIAIFVQACIIVEARP
                     QDLVTGVHGMADIIGRSLPPDFDQFLPSLQPALETIDLAIFGTVFGVILAFPLAVLAA
                     ANVTPARWLYVAARIVISICRVVPDLVWALLFVTAVGLGPFPGALALAVHSIGMLGRL
                     FAEVIEDMDMGPVEALTLTGASRMQVFTHAVVPGVLPSLLGISLFRFDENLRSSLVLG
                     FVGAGGIGFQLLTAMNLFQYQTVSYLIIITFVLVVCAERFSAFLRKRVH"
     gene            complement(17090..17395)
                     /locus_tag="VGC69_04755"
     CDS             complement(17090..17395)
                     /locus_tag="VGC69_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0294632.1"
                     /translation="MLYVFHLLDKPGVGELRLKTRPEHKQYLAAVADRIAFAGPLFAE
                     DGQTMAGSLLVIDFPSREAALQWQAGEPYTKAGIYGASSIHAFTNLWPQKVGFPPAA"
     gene            complement(17449..18699)
                     /locus_tag="VGC69_04760"
     CDS             complement(17449..18699)
                     /locus_tag="VGC69_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEY0294633.1"
                     /translation="MCEQCKPDDATATPAGSRRRRLWDLPHACHCPVIGVCLPLNTLR
                     SLVNKTLGGQAVAEDYEIHVGAVAECTHRNRLSKRLQDELDKRYAATVQAFHTAKTTV
                     AVAEMWVAAIRQGDVAGAFWAALTHPRCDEVLQDVLLRDMHMLQHQAGAATRVDIARF
                     KALQKAHADLDLELEKTRQRYTRALAEKTAEADRLSAEAVQLRAAGVAKDSQIAFLTQ
                     DMETLKATLADPAQAIKLQKKIESLASRQAELEAQNQALRQQLADALKASASRAAETA
                     RAASSLPTPAGALDGNAPAVPPSLQHQVVLCVGGRNGNIANYRAVIEGIGGHFAHHDG
                     GLEDKHGALDASLAAADLVICQTGCISHNAYWRVKDFCKRTGKRCLFVENPSTSSLVR
                     ELHQNLAPAQAPLEGESGVPLQSA"
     gene            complement(18878..19852)
                     /locus_tag="VGC69_04765"
     CDS             complement(18878..19852)
                     /locus_tag="VGC69_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294634.1"
                     /translation="MQSMQWLGRLCAAALLGAAALGSAQAAFPDKPITFIVPYSPGGT
                     TDLVAREVGRKITQKLGVSVVIENRAGAGGNIGMDAVARAKPDGYTIGLGAISTNALN
                     PFVYKSMPFDPRKAFDGISMLGNSSIVLEVNPSIKVNSVDEFIAYARQHPGLAFATAG
                     AGTSMHLAGVMFDQLAKTSLVHVPYKGSPPALTDLMGGQLFVMFDNLPASLPYIQAGK
                     LTALAVTNSERAKSLPNVPTLKELGYGGATVDPWFAVYGPAGLPADVKKSLSDTIQWA
                     LADADVKSKLELAGFSPRGSSAEEVDKLSRDQYETFKQLSEHVKLSTD"
     gene            20037..20399
                     /locus_tag="VGC69_04770"
     CDS             20037..20399
                     /locus_tag="VGC69_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEY0294635.1"
                     /translation="MNIFKAILSKIFPSDHPANDPASTGVTPPAAVASAPPVDITAVL
                     TAMQANNPQKLNWQTSIVDLMKLVGLDSSLDARKTLAGELGYTGDTNDSATMNIWLHK
                     AVMEKIAENGGTVPDSLK"
     gene            20544..21299
                     /locus_tag="VGC69_04775"
                     /pseudo
     CDS             20544..21299
                     /locus_tag="VGC69_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
     gene            complement(21277..21573)
                     /locus_tag="VGC69_04780"
     CDS             complement(21277..21573)
                     /locus_tag="VGC69_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294636.1"
                     /translation="MTSLRLLLLLPPALLLAACASHMSEVEPVGKDRYRVSYNAGMHA
                     ASWVEVKNTARERARAYCSGQGLRMTQPETYSNSATGLGAKEATLVFTCEKPPA"
     gene            21707..21955
                     /locus_tag="VGC69_04785"
     CDS             21707..21955
                     /locus_tag="VGC69_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294637.1"
                     /translation="MEIQEQIAVIVHTISHQGGRIDALHAALASLLLMAKETPQLREA
                     IESRLEQQYAGLLAGSENPQYVAGFESMRDALLAALKA"
     gene            22187..22263
                     /locus_tag="VGC69_04790"
     tRNA            22187..22263
                     /locus_tag="VGC69_04790"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22221..22223,aa:Met,seq:cat)
     gene            22451..22924
                     /locus_tag="VGC69_04795"
     CDS             22451..22924
                     /locus_tag="VGC69_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294638.1"
                     /translation="MAGMGLLMAGCADTGLMEKQAQGLSDSFTVSTDTDSAYRRGIEY
                     VRVCHKSRPHPYGATYGSLRDSDMEGGSDDPFAFLSSDKDKDSKSKAGGRIRVYKVGQ
                     EAKILELIEARSVSVAPVETRVTVRVLGTDAWDKAELAAAHQSIQSATPTCRPLE"
     gene            22928..23359
                     /locus_tag="VGC69_04800"
     CDS             22928..23359
                     /locus_tag="VGC69_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294639.1"
                     /translation="MGAPYRQFTGMLRMIAALNGLASLMLTAFSLGILGGDVDPPDLS
                     EPLEYFLAGFGACGLALLLIFLTQLWREHRQRLGRTPGGRLGLILAVLAFAGGVAGFG
                     MGCWVSADASADDGQGDGTPTAYTAGKPLVSHLGKKILFYR"
     gene            23467..24762
                     /locus_tag="VGC69_04805"
     CDS             23467..24762
                     /locus_tag="VGC69_04805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEY0294640.1"
                     /translation="MSISQQAFLRDAMRRLNLTRDLFAARIGVKRRALDTWLLPEGSQ
                     EFRTMPEVVERFVGEIVQNRDLLEKYTQSAQSGPLRDRISSSGKHQLLSVDQFTRESA
                     EDLFHVADMMQPIARRQKVSRVLEGAVLGNLFFEASTRTRVSFGSAFCRLGGSVCDTT
                     GFTFSSMAKGESIYDTSRVMSGYVDAMVIRHPEKGSVAEFAAATNIPVVNGGDGPGEH
                     PSQALLDLYTILTEFSRLGKLLDGAHIAMVGDLKYGRTVHSLIKLLALYKGIKFSLVA
                     PRGLEMPDYIIEQAARNGNVIEQKSSLAEGLPGADVIYATRVQKERFVGEDAESVYTP
                     DFQVGKATIDAYCSPETIVMHPLPRDSREGANDLSIDLNHDPRLAIFRQTDNGIPIRM
                     AIFAVLLGVEDLVQHSMRDVTWKHPSHVGPDDSSFHGLD"
     gene            24787..25821
                     /locus_tag="VGC69_04810"
     CDS             24787..25821
                     /locus_tag="VGC69_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0294641.1"
                     /translation="MIRTHSLLASLALCAAAASASSAAAPAHGGPAYGPELEGYAYPY
                     PVHRFDFTSQGEALHMAYLDVRPERPNGRTIVLLHGKNFCAATWEGSIKVLSSAGYRV
                     LAPDQIGFCKSSKPAHYQYSFQALARNTHALLESVGAANTTIMGHSTGGMLAIRYALM
                     YPAQTRQLVLVDPIGLEDWKAKGVPSLSVDQWYARELKTSADGIRRYEQSTYYAGQWR
                     ADFEPWVQMLAGMYRGPGKKIVAWNSALLYDMIYTQPVVYELGQLSMPVLLMIGDKDT
                     TAIGKDAAPPEVRARVGRYPELAQQTKAAIPHVSLVEFPDLGHAPQIQDPQAFHAALL
                     KGLAAGAAIR"
     gene            complement(25858..>26656)
                     /locus_tag="VGC69_04815"
     CDS             complement(25858..>26656)
                     /locus_tag="VGC69_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294642.1"
                     /translation="GRIVGNITSGLLLGFLLSRPVASFLADYLGWRAVFLFAAALMVI
                     VTLVVFKTIPSRRPTHQASYGQLLASLGHLFATLPTLRYRAFYQAMMFGSFSLFWTAV
                     PVELTRIYGLSQTQIALFSLIGATGACSAPLAGRLADAGYTVRATMVALAVGVLAYAP
                     ALAHPGWGMVGLVLTGIILDFAVQMNIVLSQREIYALDATSRNRLNALFMTGIFIGGA
                     IGSACASPLYEYGGWPAVSILAAIFPALALLHYLFIGRPHAIRQMAA"
BASE COUNT         4689 a         8544 c         8794 g         4629 t
ORIGIN      
        1 ctcaagtcgg aattcccggc catcaccgag ctgcgcagcg ccgtgctgtg gtggaagggg
       61 cactctaagg ttgtccctga ttattacgtg gacaagctgc cgaccaaccc ctggatccac
      121 cagcctttcg aagactatga cagcctgcgg ccgcatcagc tcgaggcgtg gatacggaag
      181 gggctgaaag gctgaaattg tgctagaatc aacagttacc tgacttaggc ctacacttgg
      241 tggtgattat ttgggcgggg ccggcccagg cagtaccctt agaatttaag cagctttgcc
      301 tcatggcgga cgcaatagtc ggacgcaaga gacgcaaggc gattgaccca aatttccaag
      361 aattgagctg aacaatgcct atcgttcgcc tgaaagaaaa cgaacccttc gaagccgccc
      421 tgcgccgctt caagcgcacc attgaaaaga ccggccttct gaccgagctg cgcgcccgcg
      481 agttctacga gaagcccacc gccgagcgca agcgcaagct ggccgccgcc gtgaagcgcc
      541 actacaagcg catccgcagc cagcaactgc ctccccgcat gtactaagct gcccgcagcg
      601 ttttgcatgc ctcgatcgcg cctggcccag ccaggcgcga ttgtttttag ggtgtgcaaa
      661 agatactctt aggcaacccc ccacctgatt ttccagtcta ttgatccccg agtcattcat
      721 tcaggatttg ctcgcccgcg tcgatgtggt cgacgtggtc gggcgctatg tgcagctgcg
      781 caagggcggg gccaatctgc tgggcctgtg ccccttccat aacgaaaaaa gcccgtcctt
      841 caccgtcagt cccaccaagc agttctatca ctgcttcggc tgcggcgccc acggcacggc
      901 catcactttc ctgatggagc acaccgggtc caatttcccc gaggccgtcc gcacgctggc
      961 cggccaggtg ggcatgacgg tgcccgagga gccccgcagc ccgcgccagc aggccgaaac
     1021 cgtccgccgc aaggccgagg tgtcgcgcca cacgcaggtg ctcgaccagg cccaggtgta
     1081 ttacctcaag cagttgcgcg cctcgcccgc ggccatcgcc tacctcaagc agcgcggcct
     1141 cacgggcgag atcgcggcgc atttcggcct gggctggtcg ggcaccgacc ggcacggcct
     1201 gtccaaggta tttgacgatt acgccgatcc ggtgctggtc gagtccgggc tggtcatcga
     1261 gtccgaggac ggccggcgct acgaccgttt ccgcgaacgg gtgatgtttc ccatccgcaa
     1321 tgcgcgcggc agcctgatcg gctttggcgg gcgcatcatc ggcgacggca agcccaagta
     1381 cctcaattct cccgagacgc cgatcttctc caagggccag gagctctacg gcctgtggga
     1441 agcgcgccag gccatccgcg aggagggcat ggtgatcgtg gtcgagggct atatggacgt
     1501 ggtgggcctg gcgcagctgg gcatcgccaa tgccgtggcc gccctgggca cggccaccac
     1561 gcccgaccat gtcaagaagc tgctgcgcgc cagcgaccgg gtggtgttca gcttcgacgg
     1621 cgacgccgcc ggccggcgcg cggcctggcg cgccctgcag gcttgcctgc cggtgctgcg
     1681 cgacgacatc aatatccgct tcctgttcct gcccgccgaa cacgacccgg actcgtatgt
     1741 ccgcgaattc ggcgccgagg cgttccgggc ggtgctgggc gaggcctcgg ccttgtcgcg
     1801 tttcttgctg gacgaactgg cttcccacca cgacctgggc gaggccgagg gcagggcgag
     1861 ctgcctgcac gaggccgcgc ccttgctggc cgctattccc gattgcgccc tgaaggtgca
     1921 gatcgagcgc gaactggctc gccaggtgca gctcacgccc gaggaaatga cccaggtcct
     1981 cgtccaggtc caggcccggc aggcgccccg ggggcccttg cccgtgccgg ggcaggaagc
     2041 ggcgccggcc cccccgcacc aggccggccg gacggacgcc ggtccaggcg ccgcgccgcc
     2101 agcctgggag tcgaacgacc tcccggcgga cgtgggctac gagcccgaac cctggcaggg
     2161 ggagggcggc tggtccgggc agcccacggg cggacgcaga aatgggggcg gattctcctc
     2221 ccagaacggc cagaatcgtc cctggcgggg ccgccgcgac gagaggccga ccggtttcgt
     2281 ggggcgccgc cccatgccgt ccatggccaa gcgcctgctg tgcctgctgc tggccaatcc
     2341 gggcctggtg gacggtatgg gcgaccagca gcttgaagtt atcgaccatg atccccaatt
     2401 aggtctggtc agggatctga tcatgctggc ccaggccagc ggggcgcgcc atttgggggc
     2461 cttgctgcag gcggccgagc cggattccga tctcagcgtg gtcctgcagg gcctgcgggc
     2521 cgaggtgctg gtcatggaag acctgcccga cccgcagggc gaatggaact acgccctgcg
     2581 gcgcatcgag cattcctggg tcaaggccga aatggcccgt ctggccgagg aaggcttgca
     2641 aacggacgag tcgcgccagc ggtttcaaga attagggggc cggatgacta tcctccgaaa
     2701 tgcgggcata cgctgattgc ggtaaaattt aaggtttcct gttgcttcgc ggcgatgcgc
     2761 ttgcgggcgg cagggtataa ggtaaatagg tctcccattt tttgggtgcg acgacggaag
     2821 cgagttcatg agcaaatcta ctggtaaaac cacctcggca atcgacgcag aaaagaccaa
     2881 ggcaagcaag gccgtcggga aggcggcgcc tcctgcggcg gtaaaaaagg ctgacgccaa
     2941 gcccgcggct acgaaggtcg ccgccaagcc cgcagccaag gccgccactg ccggggctac
     3001 cgcggtaaaa accgccggca agcctgcggc caaggccgcc gccaagagtg cggtggccaa
     3061 gaaggctgcc ccggccgccg ccgacaaggc gccgcgcgcc aagaaggcga gcgcggccgc
     3121 ggaagacaag ctggccgacc tggtcgccgc ccccaaggtc ggcggcacgg gccgtcgtcc
     3181 cggccgtccc gccaagaatg ccaatcccga cgccgaaggc ttcgaagatt ccatggacgg
     3241 cgaaggcgaa gtcctgcccg agctcaagcc ggtcaagcgc ggcggcaagc gcggcaaggg
     3301 cgacgccaag gaactgctct cccgcggccc gctcacgccc gaagaacacg aagcccgccg
     3361 caaccgcctc aagcagctca tcaagctggg caaggaccgc ggctacctca cctacggcga
     3421 aatcaacgac cacctgcctg acgacctggt cgacgccgaa gccatcgacg gcatcatcag
     3481 caccttcagc gacatgggca tcgcggtcta cgaccaggcc cccgacgccg acaccctgct
     3541 gatgagcgag aacgcgccgg tcgcgtccaa cgacgacgac gtcgaggacg aagccgaagc
     3601 cgcgctcacc accgtcgact ccgatttcgg ccgcaccacc gaccccgtgc gcatgtacat
     3661 gcgcgaaatg ggttcggtcg agctgcttac ccgcgaaggc gaaatcgaga tcgccaagcg
     3721 catcgaggac ggactcaagc acatggtcat ggccatctcg gcctgcccga cgactatcaa
     3781 cgagatcatc agccacgcca cccaggtgcg cgaaggccag aagcagatcg acgaagtggt
     3841 cgacggcctg gtcgatcccg aggacggcga ggaatacgcc ggcgccggcg tgaccgccga
     3901 cgaggacgag aacgacgaag gcccggccgg cggcatgtcg agcaagcagc tcgaagacct
     3961 gcgcgtgaag tcgctcgaga agttcgacac ggtggtgcgc cagttcgaca agatgcgcct
     4021 ctcgtacgag aaggaaggct acaagtccga cgcctacacc aaggcccagg aatccatcca
     4081 gaacgaactg atgggcatcc gcttcacggc caagatggtc gagaagctgg ccgacaccct
     4141 gcgcaaccag gtcgaggaag tgcgccagct cgaacgcgcc gtgctccaca tctgcgtgga
     4201 tcgcgccggc atgccgcgcc cgcatttcct caaggccttc cccggcaacg aaaccaacct
     4261 gcagtggatc ctcgacgagg tggccgccaa gccgccctat gccgaaacgc tcgaacgcca
     4321 gatcccggcc gtgcaggaac tacagcaaaa gctcatcgac ctgcagaccc gcgtcgtgct
     4381 gccgctgaag gacctcaagg acgtcaacaa gcgcatggcc accggcgaag ccaaggcccg
     4441 caaggccaag cgcgaaatga ccgaggccaa cctgcgcctg gtgatctcca tcgccaagaa
     4501 gtacaccaac cgcggcctgc agttcctcga tctcatccag gaaggcaaca tcggcctgat
     4561 gaaggccgtg gacaagttcg aataccgccg cggctacaag ttctcgacct acgccacgtg
     4621 gtggatccgc caggccatca cgcgctcgat cgccgaccag gctcgcacca tccgcattcc
     4681 ggtccacatg atcgaaacga tcaacaagat gaaccggatc agccgccaga tcctgcagga
     4741 aacgggcgcc gagcccgatc ccgcgaccct ggcgcagaag atggacatgc ccgaggacaa
     4801 gatccgcaag atcttgaaga tcgccaagga gccgatctcg atggaaaccc ccattggcga
     4861 cgacgacgac tcccacctgg gcgacttcat cgaggacaac tccaccctgg cgccttccga
     4921 cgccgcgctg catggctcca tgcgcgacgt ggtcaaggaa gtgctggact cgctcacccc
     4981 gcgcgaggcc aaggtgctgc gcatgcgctt cggcatcgaa atgagcaccg accagacgct
     5041 ggaagaggtc ggcaagcagt tcgacgtcac ccgcgagcgc atccgccaaa tagaagccaa
     5101 ggcgctgcgc aagctgcgcc atcccagccg ggccgacaag ctcaagagct tcctggaagg
     5161 ccagtaatcc ggcttggccc ggaaggcgca tgcgccttcc gggccggcta cccgctttcg
     5221 gccctctagc tcatgcctgg ttagagcagc ggactcataa tccgttggtg ctcggttcga
     5281 ctccgagggg ggctaccaat aacctgttta acgacgccta agaagtctag aaatccctag
     5341 agaatcaagt atctagggat tttttttgtc tatagcagtc cgaagtggac tattgccata
     5401 ccatcaatgg tgtttgtgtc tttgctggta tctgctcaaa aggcaatccg ccagcaagct
     5461 agactggata gcccaatatg gcacagaggg agtagtgcaa tcgttggggg acagtagatt
     5521 taggaccttt ccgttacctt tcagaaaagt gaggtgatct acatggcttt gattagttgt
     5581 cctgaatgtg cacgacaaat ttccgataag gccgccgcgt gcccatcatg tggctgccct
     5641 atctctgtat ccgaaaaaat gcagcagtca acggcggcaa tgaatgctcc agttcaaacg
     5701 gtaaccctgt ccaaaagcag aggggtgtac atcatccttg ggcttttatt cgggtatctg
     5761 ggatttcata atttctatgc cggtcagcat ggccgtggag caatcaaact catcctactt
     5821 ctcgttaccg tggtgctgga tggagcaaca ggttttcaca ccggattctc actcatcgtt
     5881 atagtcctaa caacaatctg gtctcttctt gaaatcttat tcgtgacacg agacgctgca
     5941 ggcaacgttt tttcctaata cctcccccaa aaaattgatt cattgagggg aggtagggcc
     6001 aatttgaatg ggggtaggcg ctggtgcagc ttgggtcgtt gttgcctgcg tggaggcttg
     6061 ctgcgcatcc tcaggcttgg ctgggacatc cttatcattg ttcggcttag gtgggacgac
     6121 agtaatggtc tgccccatca ggacggttat cagcggggga tagtcgtagg cagaacccgt
     6181 tcccatatct acggcggctc cgatgatccc gccagccaag atattccccc aggccattcc
     6241 cttggttgat gattcgaccg ttgcgatacc aggatctacg ccgtccttcg tacatttcag
     6301 gtggatcggg ttatagcttc tgtggatcgt gacggttccg ggcgtagtca cgaaccacgt
     6361 tcctttattg ctttctagct ggcaaagggc gtcgctcaac tgactcgagt tcgatagcgt
     6421 ttgaacagac agtgactggc tagatccgtc cacgatagat gcgcatccag aaagcagggc
     6481 agcagagact gaaacgctaa gagtctttat tctcatgttt tcacaaggca gttaaagatg
     6541 gtgatacctc tgcgggttat tgcctcagtt tcaccgttgc gtaaagacgg tcagcaccgc
     6601 gccctcgatt gaatcctgga tgcgctactc gttcgcaaga aaatactact cggattcttc
     6661 ccgtcgaagt cgagcgcttg cgcctcgaag gcggctggcg ctaatgacga ggcccaagac
     6721 tgttgcggcg gccgccagcc acagcgccag ttgaatggcc tgggcgcttt gcgtgatgct
     6781 cattagcccg tctgcgccag ttattgcctg cggcgcatat accgacagga tgaccccaac
     6841 gagggtcgtt cccaggcatt ggccgaaggt gcgcactatc gccaagaccc cggacgcgtt
     6901 gccgctgcgc tcgcgcgagg cgtttgccat catctcgcgg ttattgggac tttggaagat
     6961 gccgaagccg gcgccgcaaa gcagttcccg ccaactgata tcccatgcat tggcgtgccg
     7021 aggcagcagc gcgaggagcg cgagcccgat cacaaaaatg ctcaatccca tcgtcgcaag
     7081 cgcggccggc tgatggcggt cggccagccg tccggcatgc ggggccgcca gtatgatgcc
     7141 gatcggccag ggtgtgaaaa gcagcgccgt gacgaacgcg ctatggtcgt aaacgttttc
     7201 gaacaatatc ggcagtgcga tgaacgtgat tccctggccg acgaacgacg cgagggacgt
     7261 cagcgccgcc atggaaaatc gcgaggacga gaacatgtcg agcggtagca gggggtcctg
     7321 ggcgcgtcgc tgccgccaga cgaacgccgc gccggccgcg gcggcggtca atccgtaggc
     7381 aatgggtgtc agcacggaaa cgagtgtccg ttccccggca ccgagccgcg aacaggcgtc
     7441 cgcggtcaga atcagtgccg ccatcgcgat ggcggacagc aaagcccccg cgatatcgaa
     7501 tggcttgtcc gtgggggcat gttcgccagg gatggtgcgc agcgccagcg ttacgccgac
     7561 gatgccgagc gggacgttga tcgcgaagat ccattgccag cttaaaaccg acagcaaggt
     7621 gccgcccagg atgggggcga tggccgtgct ggcggcgata agcagggcat gcaagccgag
     7681 aatccgtccg agcaggcgat tcggaaagac cgaacggtgg atggcgggcg caatgctaag
     7741 cgtcgcggcc ccgcctatgc cttgcagcac gcgcatggcg gtcagccacc ctagcgacga
     7801 ggacagcgcg cagccgagcg acgccagcgt gaacacggcc aggccgcccg cgaacaaggc
     7861 tcgaaagcct agccgcttcg cgagcgcggc gaatgtcgcc aaggcgacgg cgaccgacag
     7921 cagataacca ttggacaccc agatcgccgc gcctgcggga acgtccagcg cccttgctat
     7981 ctgcggcaat gcgatgttga cgatcgatcc gtcgaatacg gccatggtcg tcgaggtcat
     8041 cacggcgatc atcgccagac ggcgctttct accgggcaat ccctcgtctc ccggcaagtc
     8101 ggagaacaat tccatgtaac aagatcctta tgccgtggcg agccttgcag aatgcgcagg
     8161 ggcgcggtcg cggcattgac gggctgtgcg ctcgtcggtg agtgggtcca gggaatatat
     8221 cgatgcgcaa ggcatggcgg aaggcgcagc atttgcactt ataaaatgcg ccagacgcca
     8281 tgcctggatg gcgcgtgcca ggagcggaca tgcccgaacc cgatttgaac ctgctcaccg
     8341 cgctcgacgc cttgcttgcc gatggcagcg tggccggcgc cgcgcgccgg ctgggattga
     8401 gcgcctcggc aatgagccgg acgctggccc ggctgcggga gacgctcggc gatccgctat
     8461 tggtccgcgc ggggcgcggc atggtattga ctccccacgc ggagacactg cgacaacgca
     8521 cccggaatgc aatgcatgaa gcccgcgcct tgctccgtcc ctccacggcg gagccggatt
     8581 tttcaacgtt gcagcgaacc tttaccattc gcgccaacga cggctttgtg gaggtcttgg
     8641 gcgctccgct catcgtcgcg gcgatggcca tcgcgccgct tgtgcgtctg cgcttcgcgc
     8701 ccaagcctga gaagacggca gtgcatttgc gggagggttc ggccgacctg gagatcggcg
     8761 tcctgggcga aatggggccc gaagtccgcg tgcaggcctt gttccgggat cgctttctcg
     8821 gcgtcgtcag gcggggccat ccgctcgaac tggagcacga gataacggct gaacggtatg
     8881 tcgccttcgg tcatgtcgtc gcttcgcgac gcgggcgcac aaacgggccg gtcgatgaag
     8941 cgctggctgc tttggggttg gaacgaacgg tcgtcgccat ggtgccgagc tttcccgccg
     9001 cgctggccgt ggctcgggcc tccgatctgg tcgcgctcgt gcccgcgtcg ttcctgggca
     9061 atcaacctgg acatcgagac tcggcctcgt ccagcacgac ctatgccttc gagctgcccg
     9121 tgacgaccga gaaaatcacg atctcgcaga tgtggcaccc tcgcctggag gccgacccgg
     9181 tccatcgctg gctccggcaa ctggtgctgg ctgtatgccg ggaaagggtg ccgctgtaaa
     9241 gcggcagcgc ttcagaaggg ttccttgtag ggacgcaggt ccagttcctg ggtccatgcg
     9301 ctgcgatgtt gctgatgcaa ttgccaatag gtgtcggcca gggcttgcag ctgcagcagg
     9361 ccatcgtcgc cggcctgctg gacgcgctgg ggcgcattgg tacgcagtcg ttcgccatcg
     9421 atgccgccgt cgatgacgac gtgcgccacg tgtattcctt gcggaccgaa ttcgcgcgcc
     9481 aggctctggc tgagcgagcg cagcgcggcc ttggcggcgg caaacgccgc gaatggcggc
     9541 cgcccgcgca gggcagccgt ggcgccggtg aacaacaggc tgcctcggcc tgtgggtgag
     9601 gcagggtcga cgccattgtc cagcagggcg cgcagcaccg cctgggcaaa caggaagccg
     9661 cccagggtgc tggtgcgcca ggcctgctcg aattgggcgg cgctcaggtc cagcgaggga
     9721 gctcgcgcgc cctcggcggc attgaatagg cctaggtgca gcggccccaa ttcgcgcacc
     9781 tgcgcggcgg cccgcgccag cccggcttca tcggccacgc tggcgggcag agccaaagcc
     9841 tggccgccgg cggcccggat ctgatccgcc aatgcttcca gcctggcggc cgtgcggccg
     9901 gtcagcgcca cggcgtagcc tgcttgcgca aagcgatgcg ccagggcgcc gcccagtccg
     9961 gcggctgcgc ccacgccggc gatccaggca acagggaggg aaggggatac gctcatagga
    10021 cggttcaggg cagtcatggc cggttcacga gccggcgcga tcgagttctg caaagtcgtc
    10081 gtcggacagt tccaggcccg ccgccgcgat attcgcttcg agatgcgaga ccttcgaggt
    10141 gccggggatg ggcagcatga ccgggctgcg gcgcagcagc caggcgagtg cgatctggct
    10201 gggcgatgcc ttgtatcgct tcgcaatctc gcccagcgca ttgcctggct tggccagatc
    10261 gccagacgcc aagggaaacc aggggatgaa gccgatctgg tgcttcgtgc agtagtccag
    10321 caccgcttcg tgttcccggt cgatgagatt gaatcggttt tgcacggtgg ccaccttgaa
    10381 gaccttcgag gccgcctcaa tgtcggccac ggacacctcg ctcagtcccg cctggcggat
    10441 gatcccatca tccaaaaggg atttgaccgc gccgaactgc tcgtcgcgcg gaacccgcgg
    10501 atcgatccga tgaagttgcc agagatcgat ctgctcgacg ccgagctggc gcaggctttt
    10561 atgcgcctgc tggatcagat aggcgggatc gcctttcggg gaccattggt ccggcccttc
    10621 gcgcgtcagg ccggccttgg tggcgatgcg cagccccgta taggggtgga gtgcttcccg
    10681 gatcaagggc tcggacacat ccgggccata tgagtcggcg gtatcgatga agtcgacccc
    10741 caggtcgggg agccgcttca gcgtgcggat ggcttcggcc cggtccaacg gctggcccca
    10801 tatgccgggc ccggtgatct gcatcgcgcc aaagccgagg cgatggattt caaagtcgtt
    10861 gccgataaga aaggtgccgg caagggcggc gtttgcgttt gtcatgagtc gaactccggt
    10921 gaaggctgga agccgctggc gccagtggct tccagatagt tgaatcaggc gcggcgagct
    10981 tcgcgggcga cgaaccgcgg cggctcgaag gccgttattt cgggcagggg gcctgtccat
    11041 cgcgccacgc gcacgaggca ggtctgcgcg gcgcagcctt gcgaaaggcg ggatgccggc
    11101 agatccatgg taagcgcatt gggattgccg tgcttgtcga gcaggggcgt ttcgccggac
    11161 gggtcgaacc aggcgccggt cgccagtttg acgacgccgc gcatcaccgc gtcgtccagc
    11221 tgcgccgccg cgatgcaagc gccccgcggg ctccagacgc gcacggcctc gccgtcctgg
    11281 atgccgcgct cgcgcgcatc ttccggatgc atcgtcaacg gttcgcggcc ctgccgccgg
    11341 acgccctggc tcaaggccga atggtccagc tggctgtgca gtttcgtcgc cggctggtcg
    11401 gagatcaggt gcagctgctg cgcgtccgcc gcgcccagcc attcggcggg ttcgagccat
    11461 gacggatgcc cggggcagtc ggcatagccg aacgaggcga tggtctccga ccagatttcg
    11521 atctttcccg agggcgtctt cagaggatgc gcgccggggt cttcccggaa gcgccgcagc
    11581 aagggcgtgg gcggcgtcgt tccctcgaaa tcgaccaggc cctgttccca gaagccgtcg
    11641 aacccaggca gttcgacgcc cgcggcctgg gctctcggca gcgtttcgtc gtatagggcg
    11701 cgcagccagg cgtgcgcgtc gcgcccttcg tcgtagggcg cttgcgcgcc caggcgagac
    11761 gccagcgcgc ggaagatctc gaaatcgtcg cgtgcttcgc ccgcgggcgg cagtacccgg
    11821 cgcatggcca ccaggtgcgc ctcgcgcgat gaaaagccga tgtcgtcgcg ttccagcgtc
    11881 gtcgtcgccg gcagcacgat gtcggccatc ttggcgtgcg cattccagaa ctgttcatgc
    11941 gcgatgacgg tgtccggctt gcgccacagg gtcgcgagcc ggttcagatc ctggtggtga
    12001 tgaaaagggt tgccgccggc ccagtagacc agccggatgt cggggtagcg gtgcgagcgg
    12061 ccgttgtagt cgaatgcctc gccgggccgc gccagcatgt ccgcgatgcg cgcgaccggg
    12121 atgaaggcat ccaccgcgtt gcgtccctgc ggcagcgtcg gcccttggaa acgcgcgctg
    12181 cggtggccta tcgaattcgt cgagccgtag ccgaaagcga agccgccgcc gggcaggccg
    12241 atttgcccca gcaccgctgc cagggccgtc agcgcccaga atggctgttc gccgtggtgg
    12301 gcccgctgca gcgaccacat tgccgtcacc agcgtccgtt cgcggcccag cgcatgggcc
    12361 agttcggcga tctgctcgcg cggggcgccg gtgatgcgcg atgcccatac ggcatccttg
    12421 gcgatgccgt cggcctggcc gagcaggtag gcctcgaaac gcgcccagcc gacacagtgc
    12481 gaggcaagaa aggcgcggtc gtggcgcccc tcggccagca cgccgtgcgc caacgcgagc
    12541 atcagcgccg tatcggtgtt gggccggatc ggcagccact gcgcctgggc gccgggttcc
    12601 gcatcgttgc ggctcggtcc gatattgacg atgcgcacgc cctgccgggc caggtggcgc
    12661 atggcgtcgc gcagccgatg gcgccccgcg ccgccggagt cgctctgggc gctcttggcc
    12721 ggcatgccgc cgaagcagac cagcagcctg gtgtgccgcg ccagcgtttc ccaatcgtcg
    12781 ctgttggaca ggatggcgtc gatgggcgcg acgatgcggg gcatcagcac gccgcccgcg
    12841 ccgtaactgt aggagtcgac gctgcgcacg tagccgccga gcacgttcag gaagcggtgc
    12901 agatggccga ccgagtggtg gaaacgcccg gcgctggccc agccatacga gccgccgaag
    12961 atggcctggt tgccgtgggt gtcgatgacg cgccgcagtt cttcggcgac caggtcgagc
    13021 gcgtcatgcc acgacacttc gacaaagggt tccgtccccc gggctgtccg gctggcgcgc
    13081 tggttttcca gccaaccggc gcggatcgcg ggccgcagga tgcgttgcgg cgcattgttg
    13141 gccgccagca tgtgcaggcc gatgggcgat gggtcggggt cttgcgccca gggcgccaag
    13201 gccggattgc cttgcgcgtc atgccggact tcgtagaggc cccagtgcga ggcggtgtag
    13261 cgggtcggat tcatggctgg gcttcatggg ctggctgctg cggatggcga tttcgcgtta
    13321 ccgatgcatg attgcacgcc gtttgccggc catacaagcc atggtccaag acggtttttc
    13381 atggaagggc gccgcgcggc tcgtcgcggc ggttgccatt tgggcacaat atcgggctat
    13441 ggacatgaat acaggtgaaa cgatgcaggc aatattgcag ggcaagacgg cgatcgtgac
    13501 gggcgccgcg ggcgatctcg gcgcggccat cgcctgctgc tttctcgagg cgggcgcgaa
    13561 cgtggcttgg ctggacaggg atgccgaaag ggcgagcgcc cgggcgcagg ccggtcccgc
    13621 gcccgagcgc tcactcgcgg tgcattgcga catcgccagc gcgtcctcca cccaggacgc
    13681 ggtcgccagg gtggccgaac gctttggcgg gatcgacatc ctggtcaaca acgccgcgac
    13741 cgtcagcgcg agaaagcccc tggccgagct cgacatcgac gcgtggcggc acacgctgga
    13801 cgtgaacctg aacggcgcct tcctgatgtc gaagtgggtc attccgcatc tgctgcgcaa
    13861 gcgggccggc gtgatcctga acgtggcatc gcaactcggg cacgtgacca atcccggcgg
    13921 cagcgcctac agcaccagca aggcggcgct gatcggcctg acccgttcca tcgcggtcga
    13981 ctacgcgaag gacaatatcc gcgccgtcag catttccccg ggttcggtga tgaccagccg
    14041 cttgaccgac cgctacggca gcgaggccga ggtgaacgcc gtgctggcgc ccaggcaccc
    14101 gctgggccgc atcgcgcagg tcgcggaggt ggcgcaggcc gcgctgttcc tggccagcga
    14161 ttcggcgtcg ttcattacgg ggaccgacct gctggtcgac ggcggttata cggcggtctg
    14221 aggcctgaat aaggcaagcc gaaaaacaaa ggcaaagaaa aaccgccggc gccggcggtt
    14281 tttctttgcc tgccgtgttt gccgccatag ggcggtacga ggacattgtg acgatttcaa
    14341 gacggatgaa taagggatgc ttcatttttt caggggaatg tcaccccctg gtcatggtga
    14401 aaacacttgt ggtgcctaga ctgcgcagcg ttgcttgcgg ccatgccaac cttcctctca
    14461 acgcgaccct ttgggcccag tatgaaactc tccagaatct ttgcaggcgt tcttctctcc
    14521 accggttttg tcggcggcgc ctatgccgcc ggcacctgcc cgatcaagga acccgtgcgc
    14581 ttcggcgtcg agccctacga gtccgcgcag cgcatgatcc ccgtctacaa cgacgtggcc
    14641 aagatgatcg gccagaagat cggctgccag gtgcaactgt tcatcgccac cagctacaac
    14701 gccgagatcg aggcgatgcg caacaagaag ctggaattcg cgcaattcgg cccgctgggc
    14761 tacgtgctgg cccaccaggt cgccaaggcc gaggccgtct ccagctttgt cggcccggac
    14821 ggccagccgg ccagctacta cgcgtcgatc gtgacctggc ccggcgcggg catcgacacg
    14881 ctggccgacg tcaagggccg ctcgtttgcc tactccgatc cggcctcgac ctcaggccac
    14941 ctgttcccgg cctacggcct gagcaagaac ggcatcgatc ccgacaaggg cgtgaaggca
    15001 gtctacgccg gcagccacac ggcttcgttc gaagcgctgc gcaaccacaa ggtcgaagcg
    15061 ggcgaactca acagcgaaga aatcgcgagc gccaagctgc acaacgaata cgacgacagt
    15121 gcctacacca ccctgtggaa gtccgacaat atcccgctgg atccgatgac gatccgcggc
    15181 gacctgccgg ccggcttcaa gaccaggctg gccgccgtgc tgagttcgct ggacttccac
    15241 gagctgcccg atgccgacca gaagttcctg gcggccaacg gcacgcccca gctgaagacg
    15301 gttccccaga acgatgcggc ctacaaccag atccgtgatc tgatctccac gctgcatatc
    15361 gatctgaaga agctatgagt tttgtgcaag cgacccgcgc cgatatggcg ctggacggcg
    15421 aagcgtccct ggccttcgcc ggagcggctt cggctgctgc ctccccggca gccgaagccg
    15481 gctcgagcgt ccgtctggcg gtgcgcgacc tgagcatgcg ctatgcggat ggccatctcg
    15541 cgctcaacca gctgaacctg tcggtcaacg ccggcgaaat ggtcgtggtg ctcggcagca
    15601 acggctgcgg caaatccacg ttcatgaaat gcgtcgtcgg actgaatcgc cccacgggcg
    15661 gcacggtcga agtggccggc cgggatctcg cgtccttgtc gggcaaggcg ctgcgcgcgg
    15721 cccgtttgcc gattgccctc atttcccaga acgccaacct cgtcaggcgg cgcagcgtgc
    15781 tggacaatgt ctgcgcgggt tccttgggcc ggcaccgcac cttggcgacg tccctgggcc
    15841 gcattccgcg cggcgagatc gagcccgcgc gccgctatct cgacgaggtc ggcctattgc
    15901 atctggccgc ccagcgggcg ggcacgctgt cgggcggcca ggcgcagcgc gtggcggtcg
    15961 cccgcgcgct ggcccagcgg ccccatgtgc tgctggccga cgagccggtg gccagcctcg
    16021 accccgaggc ggccgacgaa gtcatgcgcc ttctgcgccg tctggcgacg gacgatggcc
    16081 tggcggtcgt ctgcgttttg caccaacccg agcttgccat gcgctatgcc gaccgcctgg
    16141 tcggcctgcg ccgcggtcag ctgcagttcg accagcccgc cgggcaggtc tccatgcagc
    16201 aggtctccag cctctacgtc tcctgagttt cccttcatga ctcctgttct gcagaacagg
    16261 gaggcgcagc cggccgttcc gctgatgcgc accctgtctt cgatcggcat agccgtcgtc
    16321 ctcatcgcga tcttcgtgca ggcctgcatc atcgtcgagg ccaggcccca ggacctggtg
    16381 acgggcgtgc acggcatggc cgacatcatc ggccgttcgc tgccgcccga cttcgaccag
    16441 ttcctcccca gcctgcagcc ggccctggaa accatcgacc tggcgatctt cggcacggtg
    16501 ttcggcgtga tcctggcctt tccgctggcc gtgctggccg cggccaatgt cacgcccgcc
    16561 cggtggctgt acgtcgcggc gcggatcgtg atcagcatct gccgggtggt gcccgacctg
    16621 gtctgggccc tgctgttcgt gacggcagtc ggactgggcc cgttcccggg cgcgctggct
    16681 ctggccgtgc actcgatcgg catgctgggc cgcctgtttg ccgaagtgat cgaggacatg
    16741 gacatgggcc cggtcgaggc gctgacgctc accggcgcca gccgcatgca ggtgttcacc
    16801 catgccgtcg tgccgggcgt gctgccctcg ctcctgggca tcagcctttt ccgcttcgac
    16861 gagaacctgc gctcgtcgct ggtgctgggc ttcgtcggcg caggcggcat cggtttccag
    16921 ctgctgacgg cgatgaacct gttccagtac cagacggttt cctatctgat catcatcact
    16981 ttcgtgctgg tggtttgcgc ggagcgcttc tccgctttcc tgcgcaagcg cgtccactga
    17041 gccgggccgc gcctggcctg gaagcgccgg ccccggcggg cgtttttcat caagccgcgg
    17101 gcggaaagcc cactttttgc ggccacagat tggtgaacgc gtggatgctg ctggcgccgt
    17161 atatgccggc cttggtgtag ggctcgccgg cctgccattg cagggcggct tcccgcgacg
    17221 gaaagtcgat gaccagcagg ctgcccgcca tggtctgccc gtcctcggca aagagcgggc
    17281 cggcgaaggc gatgcggtcg gccacggcgg ccaggtattg cttgtgttcg gggcgggttt
    17341 tcaggcgcag ttcgccgacg cccggcttgt cgagcaggtg gaagacgtaa agcatgggtg
    17401 acctctggat tgtccttgcg tggattgcaa aatcgggttg aaaaggcatc aagccgattg
    17461 caagggtacg ccggattctc cttcgagggg agcctgggcc ggcgcgaggt tctggtgcag
    17521 ctcccgcacc agcgacgagg tgctggggtt ttccacgaac aggcagcgct tgccggtgcg
    17581 cttgcagaaa tccttgacgc gccagtaggc gttgtggctg atgcagccgg tctggcagat
    17641 caccaggtcg gccgccgcca ggctggcatc cagcgcgcca tgcttgtctt ccaggccgcc
    17701 gtcgtggtgg gcgaagtgcc cgcctatgcc ttcgatcacg gcgcggtagt tggcgatatt
    17761 gccattgcgc ccgcccacgc aaagcacgac ctggtgctgc agcgagggtg ggaccgcggg
    17821 cgcgttgccg tccagggcgc ccgcgggcgt gggcaggctg cttgccgcgc gggcggtctc
    17881 ggcggcgcgc gaggcggatg ccttcagcgc gtccgccagt tgctgccgca aggcctggtt
    17941 ctgcgcttcg agttcggcct ggcggctggc cagcgattcg atcttctttt gcagcttgat
    18001 ggcctgggca ggatcggcca gggtggcttt cagggtttcc atatcctggg tcaggaaggc
    18061 gatctggcta tccttggcca cgcctgcggc ccgcagctgc acggcttcgg ccgacaggcg
    18121 gtcggcttcg gccgtcttct cggccagcgc ccgcgtgtag cgttgccggg tcttctccag
    18181 ttcgaggtcc aggtcggcat gggccttctg cagcgccttg aaccgggcga tatcgacccg
    18241 cgtggcggcg ccggcctgat gctgcagcat gtgcatgtcg cgcagcagca catcctgcag
    18301 gacttcatcg cagcgcgggt gggtgagggc ggcccagaag gcgccggcca cgtcgccctg
    18361 gcgtatggcg gccacccaca tctcggccac ggcgacggtc gtcttggccg tgtggaaggc
    18421 ctgcaccgtg gcggcgtagc gcttgtccag ttcgtcttgc agccgcttgg acaggcggtt
    18481 gcggtgcgta cactcggcca cggcgccgac gtggatttca tagtcctcgg cgacggcctg
    18541 gccgcccagc gtcttgttga ccagggatcg cagggtgttc agcggcaggc agacgccgat
    18601 caccgggcaa tggcaagcgt ggggcaggtc ccacaggcgg cgccggcgcg agccggcagg
    18661 ggtggcggtg gcatcgtcgg gcttgcattg ctcgcacatg gggcatctcc tgtccagatt
    18721 ggcatgttct cattatgatt gttaatgaga attattatca atctattgga gaaaagccaa
    18781 acaagcaaca tccggccccg attcacggaa ataagtctta caaaatccgg tcgccgggac
    18841 ttgcggacgt cgcttgcgag ggatcgccag ccgtccctca gtcggtcgag agcttgacgt
    18901 gctcggagag ctgcttgaag gtctcgtact gatcgcggct gagcttgtcg acctcttcgg
    18961 ccgatgagcc gcgcggcgag aagccggcca gctcgagctt gctcttgacg tcggcatcgg
    19021 cgagcgccca ctggatggtg tcgctcaggc tcttcttgac gtcagccggc aggccggccg
    19081 ggccgtacac cgcgaaccag gggtccaccg tggcgccgcc gtagcccagt tccttgagcg
    19141 tgggcacatt gggcagcgac ttggcgcgct cgctgttggt gacggccagg gccgtgagct
    19201 tgccggcctg gatgtagggc agggaagcgg gcaggttgtc gaacatcacg aatagctgcc
    19261 cgcccatcag gtcggtcagg gccggcgggc tgcccttgta gggcacgtgc accaggctcg
    19321 tcttggcgag ctggtcgaac atcacgccgg ccaggtgcat ggacgtgccg gcgccggcgg
    19381 tggcgaaggc caggccggga tgctggcgcg cataggcgat gaactcgtcg acgctattga
    19441 ccttgatcga ggggttgacc tcgagcacga tgctggagtt gccgagcatg ctgatgccgt
    19501 cgaacgcctt gcgcggatcg aacggcatgg atttgtacac gaaggggttg agcgcgttgg
    19561 tcgagatggc gcccaggccg atggtgtagc cgtcgggctt ggcgcgggcc acggcgtcca
    19621 tgccgatatt gccgccggcg ccggcgcggt tctcgatgac gaccgagacg cccagtttct
    19681 gggtgatctt ccggcccact tcgcgggcga ccaggtcggt ggtgccgccg ggcgaatagg
    19741 gcacgatgaa ggtgatgggt ttgtcgggga aggcggcctg ggcgctgccg agggcggccg
    19801 cgcccagcaa ggcggctgcg cacagccggc cgagccattg cattgattgc atgaatagtc
    19861 tccattgaag gccggcgttg gaaaaaatgg atggtcgtgc cggccaggcc gagcgagtgt
    19921 acccgcgcgc catactttga tacatccagc agtgtgtcag ctaatcgatc tcatggttaa
    19981 ctcggcatgg cggcaattct gtcgttcgac cgaccatgcg aaaggagtct gtacatatga
    20041 atatcttcaa ggcgattctg agcaagatct tcccttcgga ccatcccgcc aacgatcccg
    20101 cgtccacggg cgtcacgccg cctgcggccg tcgcctccgc gccgccggtg gacatcaccg
    20161 cggtgctgac cgcgatgcag gccaacaatc cccagaagct gaactggcag acctccatcg
    20221 tcgacctgat gaagctggtc ggcctggaca gcagcctgga tgcccgcaag acgctggcgg
    20281 gcgagctggg ctataccggc gacaccaacg attcggccac gatgaacatc tggctgcaca
    20341 aggccgtcat ggagaaaatc gccgagaacg gcggcacggt gcccgacagc ctgaaataag
    20401 ccgccgcatt caaacggagg gcgaagccgg aaggggatct tccgcttcgc cctttttgcc
    20461 gcgggcgggc cattcctggg ccggcctcgg cccacggcgg ttgggcaggc gtagaatcag
    20521 caagttcgtt ttgcgagcct gccatgccga ttccatatct gcgccgtttg acggggaatg
    20581 tccgcagccc cgaggccaat cgccaattgg cttactacct gaccttcgtg gcgggcgccg
    20641 ccaacgccgg cggttttttg gcggtccagc agtacacctc gcacatgtcc ggcatcgtcg
    20701 cggccatggc cgacgacctg gcgctgggca tgatcgtcct ggtgctgcag gggatcgggg
    20761 cgctggcctc gttcctggcc ggggcggcaa gctccgccat catcatcaac atggccaggc
    20821 gttcgcacct gaccagccag tatgcgctgc ccctcatgct ggaggccgtg ctgctgatgt
    20881 gcttcggcct gctgggcggc aatatcgaac acctgcgctg gctgttcgga cccgtcacgg
    20941 tgatggtgct gtgcttcatc atggggctgc agaacgccat ggtcaccaag gtgtccaatg
    21001 ccgagatccg caccacgcac gtcaccggca tggtgaccga cgtgggcatc gagctgggca
    21061 agctgttcta ctggaatatc accgagtcca acgagagcac gcctttcgtg cgcgccaacc
    21121 gcgagaagct catcatgctc agcacgctca ttgggctgtt cttcgtgggc ggcgtgacgg
    21181 gggcgctggg tttcgcccgc gtggggttca tctcgaccgt gccgctggcc tgcctgctgc
    21241 tggtgctggc ggccgtgccg gtggccgacg acgtcttcag gctgggggtt tttcgcaggt
    21301 gaagaccagc gtggcttcct tggcgcccag gccggtggcg ctgttggagt aagtctcggg
    21361 ctgggtcatg cgcaagccct gcccggaaca gtaggcgcgg gcgcgctcgc gcgcggtgtt
    21421 cttgacctcg acccaggagg cggcatgcat gccggcgttg taggacacgc ggtagcggtc
    21481 cttgcccacg ggttcgacct cgctcatgtg gctggcgcag gcggccagca gcagggcggg
    21541 ggggagcagc aagagcaggc gcaaagacgt cacggcacta tcctggttgg caatgccgcc
    21601 agtttaccgg agcgcgcgcc gccagatggg cgtgctagca tcggcgccca atctttcacc
    21661 agggtgcgcg gtccgcgagc cgcgcattcg caatacgagt accgccatgg aaatccagga
    21721 acagatcgcc gtcatcgtcc acaccatctc ccaccagggc ggccgcatcg acgccctgca
    21781 cgccgcgctg gcatcgttgc tgctcatggc caaggagacg ccgcagctgc gcgaggccat
    21841 cgagtcgcgg ctggagcagc aatacgcggg ccttctggcg ggttccgaga acccgcaata
    21901 cgtggcgggc ttcgagagca tgcgcgacgc gctgctggcc gcgctcaagg cctgatgttt
    21961 ttaccttgat tcacagtccg catggcccaa gccttgccaa gttgcccaca gcttttgtgg
    22021 acaagtcttt ggatagcttc ggcacagacg caaaatgccc ttgccgttca agggcttgat
    22081 tggcctgagc agctttcggt caaacggggg ccgaagggct tgagcgctga aatacgcacg
    22141 caatatgcgg gccctcaaaa actgtgctag aattcaattc ttcagacgcg gggtggagca
    22201 gtctggcagc tcgtcgggct cataacccga aggtcgtagg ttcaaatcct gcccccgcaa
    22261 ccagttcggt gcacaaaggc ttggcgtata aaacacgcca ggcctttttg ctttgggccg
    22321 ggcgcgcctg gtgcataatg ccgcgcaggg aaaacgggaa aacatgatgc gaaatattct
    22381 agggacgcgc agcctcggta aaggttggat aactcaaggc tggatcacta aaggcggggt
    22441 gacgctggcc atggcgggca tgggtctgct gatggccggc tgcgccgata cgggcctcat
    22501 ggagaagcag gcccagggcc tgtccgacag ctttaccgta tcgaccgaca ccgactcggc
    22561 ctatcgtcgg gggatcgagt atgtgcgtgt ctgccacaag tcccggccgc acccgtatgg
    22621 cgcgacctat ggttcgctgc gcgatagcga catggagggc ggcagcgacg accccttcgc
    22681 ctttctttct tccgacaagg ataaagattc caagtccaag gcaggagggc gcatccgcgt
    22741 gtacaaggtc gggcaggagg ccaagatcct cgagctgatc gaggctcgga gcgtgtccgt
    22801 cgcgcccgtc gagacgcgcg tcaccgtccg cgtgctgggc accgatgcct gggacaaggc
    22861 ggaactggcc gcggcgcacc agtccatcca gtcggccacg ccgacctgcc gcccgctgga
    22921 gtaagccatg ggcgcgccgt ataggcagtt cacgggcatg ctgcgcatga tcgccgcgct
    22981 caacggcctg gcctcgctga tgctgacggc gttttccctg ggcatcctgg ggggcgacgt
    23041 cgatccgccc gatctttccg agccgctgga atatttcctg gccgggttcg gcgcttgcgg
    23101 cctggcgttg ctgctcatct tcctgacgca gctgtggcgc gagcatcggc agcgcctggg
    23161 ccgcacgccg ggcggacgac tggggctgat cctggcggtg ctggcctttg ccggcggcgt
    23221 ggcgggcttt ggcatggggt gctgggtgtc ggccgatgcg tcggccgatg acgggcaggg
    23281 cgacggcacg cccacggcgt acacggcggg caagccgttg gtgtcacatt tgggcaagaa
    23341 aatacttttc taccgataga cgccaggctt gatcggtttt aatatacgca ttgcgtataa
    23401 ttttatgcgc tgcatggtgg cagcgtatag ctgcgcgtcg ttccaaccca gcgatcgagg
    23461 ccctccatgt cgatttccca gcaagccttc ctgcgcgacg ccatgcgccg cctgaacctg
    23521 acccgtgacc tgtttgccgc gcgtattggc gtcaagcgcc gtgcgctcga cacctggctg
    23581 ctgccggaag gctcgcagga attccgcacc atgcccgagg tggtcgagcg ttttgtcggc
    23641 gaaatcgtgc aaaaccgcga tctgctcgaa aaatatacgc aaagcgcaca gtccgggccg
    23701 ctgcgtgatc gcatctcttc gagtggcaag caccagttgc tgtcggtcga ccagttcacc
    23761 cgcgaatccg ccgaagacct gtttcacgtc gccgacatga tgcagcccat cgcgcgtcgc
    23821 cagaaagtct cgcgcgtgct cgagggcgcc gtgctgggca acctgttctt cgaggccagc
    23881 acccgcacgc gggtgagctt cggttcggcc ttctgccgcc tgggcggctc ggtgtgcgac
    23941 accacgggct tcaccttctc gtccatggcc aagggcgaat ccatctacga caccagccgc
    24001 gtgatgagcg gctacgtcga tgccatggtg atccgtcatc ccgagaaggg ctcggtggcc
    24061 gagttcgccg cggcgaccaa catccccgtg gtcaacggcg gcgacggccc gggcgagcac
    24121 cccagccagg cgctgctcga cctctacacc atcctcaccg agttctcgcg cctgggcaag
    24181 ctgctcgacg gcgcgcacat cgccatggtg ggcgacctga agtacggccg cacggtgcat
    24241 tcgctcatca agctgctggc gctgtacaag ggcatcaagt tctcgctggt tgccccgcgc
    24301 ggcctggaaa tgcccgacta catcatcgag caggccgccc gcaacggcaa cgtcatcgag
    24361 cagaagtcct cgctggccga aggcctgccg ggcgccgacg tgatctacgc cacgcgcgtg
    24421 cagaaagagc gcttcgtcgg cgaagacgcc gaaagcgtct atacgcccga tttccaggtc
    24481 ggcaaggcca ccatcgacgc ttactgctcg cccgagacca tcgtcatgca cccgctgccg
    24541 cgcgacagcc gcgaaggcgc caacgacctg agcatcgacc tcaaccacga tccgcgcctg
    24601 gccatcttcc gccagaccga caacggcatt cccatccgca tggccatctt cgccgtgctg
    24661 ctgggcgtgg aagacctggt gcagcattcg atgcgcgacg tcacctggaa gcacccctcg
    24721 cacgtcgggc ccgacgattc gtcgttccac ggcctggatt gagcgagaaa ctggattgga
    24781 tcccgcatga tacgtaccca ctcattgctg gcctccctgg ccctgtgcgc cgctgccgca
    24841 tcggcatcgt ccgccgcggc gccggcgcac ggcggtcctg cctacggccc cgagctcgag
    24901 ggctacgcct acccgtaccc ggtccatcgc ttcgatttca cgtcgcaggg cgaggcgctg
    24961 cacatggcct acctggacgt gcggcccgag cgtccaaacg gccgcaccat cgtgctgctg
    25021 catggcaaga acttctgcgc cgcgacctgg gaaggcagca tcaaggtcct gtcgagtgcc
    25081 ggctaccgcg tccttgctcc ggaccagatc ggcttctgca agtcgagcaa accggcgcac
    25141 taccagtaca gtttccaggc gctcgcgcgc aacacgcacg ccttgctgga gtcggtcggc
    25201 gcggccaaca ccacgatcat gggccattct accggcggca tgctggccat ccgctacgcg
    25261 ctgatgtacc cggcgcagac gcggcagctc gtgctggtcg accccatcgg cctggaagac
    25321 tggaaggcca agggcgtgcc ttcgctgtcg gtcgaccagt ggtatgcgcg tgagctcaag
    25381 accagcgccg acggcatccg ccgctacgag cagtccacct actacgcagg ccagtggcgc
    25441 gccgatttcg agccctgggt gcagatgctg gccggcatgt accgaggccc gggcaagaag
    25501 atcgtggcgt ggaattcggc gctgctctac gacatgatct atacccagcc cgtcgtctac
    25561 gagctgggcc agctctcgat gcccgtgctg ctgatgatcg gcgacaagga caccaccgcc
    25621 atcggcaagg acgcggcccc gcccgaggtg cgcgccaggg tcggtcgcta tcccgagctc
    25681 gcccagcaga ccaaggcggc gattccccat gtctcgctgg tcgagttccc ggatctcggg
    25741 cacgcgccgc agatccagga tccgcaggcc ttccacgcgg ccttgctcaa agggctggcg
    25801 gccggcgcgg cgatccggtg agcgccgccg gttgggctca gcgccccgat gtggccgtca
    25861 ggcggccatc tgccggatcg cgtgggggcg gccgatgaac aggtagtgca gcagcgccag
    25921 ggcggggaag atggccgcca ggatggacac ggcgggccag ccgccatact cgtagagcgg
    25981 gctggcgcag gccgagccga tcgcgccgcc gatgaagatg ccggtcatga agagcgcgtt
    26041 gaggcggttg cggctggtgg cgtccagggc gtagatctcg cgctggctca gcacgatgtt
    26101 catctgcacg gcgaagtcca ggatgatgcc ggtgaggacc aggccgacca tgccccagcc
    26161 ggggtgcgcc aaggccggcg cataggccag cacgcccacg gccagggcca ccatcgtggc
    26221 gcgcacggta tacccggcgt cggccaggcg gccggccagc ggggccgagc aggcgccggt
    26281 ggcgccgatc agcgagaaca gggcgatctg ggtctgcgac aggccgtaga tgcgggtgag
    26341 ctccaccggc acggccgtcc agaacaggct gaacgagccg aacatcatgg cctggtagaa
    26401 cgcgcgatag cgcagcgtcg gcagggtggc gaacaggtgg cccagcgagg caagcagctg
    26461 gccgtagctg gcctggtggg tggggcgccg ggaggggatg gtcttgaaca ccaccagcgt
    26521 gacgatgacc atgagcgcgg cggcgaagag gaagacggcc cgccagccca ggtagtcggc
    26581 caggaagctg gccaccgggc gcgacagcag gaagcccagc agcaggccgc tcgtgatgtt
    26641 gccgacgatg cgcccg
//
LOCUS       DASWPO010000081         6013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3054022, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000081 DASWPO010000000
VERSION     DASWPO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054022"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VGC69_04820"
     CDS             <1..421
                     /locus_tag="VGC69_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEY0294643.1"
                     /translation="GQLAAWNYFESIKNPVNAEFVKKWKAYAKAQNLPNYQTAVTNDP
                     MEATYVGIHMWKQAVEKAKSTDVEKVIAAMGGQTFKAPDGFTIEMDKTNHHLHKPVYI
                     GEVQADGQFKVVWKTKGPIRAQPWSPFIPGNESKQNM"
     gene            439..2130
                     /gene="urtB"
                     /locus_tag="VGC69_04825"
     CDS             439..2130
                     /gene="urtB"
                     /locus_tag="VGC69_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEY0294644.1"
                     /translation="MHHAVFFARLRRLLLGSLLGLAALSACAYAQDAAPAASQDAATP
                     PSQAQGLDESLLSLLASDDNDEKIEAINKLSRLPDPHAVALLQALANDHVYATSDNKV
                     YISDDDGQTAQDPITGQTQPMPDGSDTLTLNNRVRSAIDAAQATAKLYSPKVADRLAA
                     AQLLLQSGDESLLPIVDQALKKETDAGVRTALQTIQAHLQLSSKDPAARRAAAQTLGE
                     SGNASFRPMLQALVQPKPDGSYDEPDASVRNAATAALKRIDQHATVIEWAGNIYYGLS
                     LGSVLLLAALGLSITFGLMGIINMAHGELLMIGAYATYTVQSVFRQWLPGLIDWYVVA
                     ALPVAFIVTALVGMALERTVIRWLYGRPLETLLATWGISLILMQAVRSIFGAQNVEVA
                     NPSWMSGGLTVMDGLVLTYNRLVIIAFALLVVFFVWLLLNHTRLGLFVRAITQNRTMA
                     DCVGVPTGRIDMLAFGLGSGIAGLAGVALSQLGNVGPALGQSYIVDSFMVVVLGGVGQ
                     LAGTVIAGLGLGWINKFLEPYAGAVMAKIIILVLIVLIVQKRPQGLFAPKGRSAE"
     gene            2228..3340
                     /gene="urtC"
                     /locus_tag="VGC69_04830"
     CDS             2228..3340
                     /gene="urtC"
                     /locus_tag="VGC69_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEY0294645.1"
                     /translation="MHLNRRPLFPPKVWAVLVVAIVVMALLPALNLAFPDGHPLHISS
                     YTIAVGGRFMCYAMAALALDLVWGYAGILSLGHGLFFALGGYAHGMYLMRAIGHDGVY
                     QSDLPDFMVFLNWKAYPWYWTFTDHFLYAMLLVVLVPGVLAFVFGYFAFRSRIKGVYF
                     SIITQALTYAAMLLFFRNDTGFGGNNGFTDFKRILGFNITESTTRAALFWITLAVLVL
                     ALIVARLITQSKLGRVLTGVRDAESRLRFLGYEPLGFKLFVWTLSAVICGIAGALYVP
                     QIGIINPGEMSTANSIEMVIWVATGGRGTLIGPIIGAGVVNSLKIWFTSAFPDYWLYA
                     LGLVFVLVTLFLPQGIVGLVRQCAARVGKMQGGNKQ"
     gene            3337..4125
                     /gene="urtD"
                     /locus_tag="VGC69_04835"
     CDS             3337..4125
                     /gene="urtD"
                     /locus_tag="VGC69_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY0294646.1"
                     /translation="MSTDNRPNPGGLDTSHGAILYLEDITVSFDGFKALNKLTLDIGV
                     GELRCIIGPNGAGKTTMMDVITGKTRPTSGSAFFGQTIDLTSLSEAEIAHEGIGRKFQ
                     RPTVFENHSVFENLELAMKTDKRVRPTLFSRLSSEQADRIAQTLDLIRLGPLAGEAAG
                     LLSHGQKQWLEIGMLLMQEPRLLLLDEPVAGMTDAETERTGELLNELRGKHSLMVVEH
                     DMDFVTQIAGGGKVTVLHEGSVLAEGPMATVQADPRVIEVYLGR"
     gene            4139..4837
                     /gene="urtE"
                     /locus_tag="VGC69_04840"
     CDS             4139..4837
                     /gene="urtE"
                     /locus_tag="VGC69_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEY0294647.1"
                     /translation="MLDIKDINQYYGGSHTLRSISLSLARGECLALLGRNGVGKTTLL
                     KCLMGVLPTASGAISLDGADITRLAPHQRAALGMAYVPQGREIFARLTVEENIVMGMA
                     TKPSSQARRIKGEIFELFPVLKTMLGRRGGDLSGGQQQQLAIARALVAEPKLIILDEP
                     TEGIQPSIIKDIGRVIHMLRQRGDIGILLCEQYFDFARELADQFVVLSRGEVVARGDR
                     SQIDSEDVRRHLSV"
     gene            complement(4894..5733)
                     /locus_tag="VGC69_04845"
     CDS             complement(4894..5733)
                     /locus_tag="VGC69_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0294648.1"
                     /translation="MRAQAAADGNLLGDYLKHRRTQLDPAAFGFPPARRRTPGLRREE
                     VAQRANISPTWYTWLEQGRGGAPSADVLDRIARALMLTDVEREHLFLLGLGRPPEARY
                     RKNESVTPRLQRVLDALNPSPALIRTATWDVVAWNRASTVMLMDYESLPPEHRNILRF
                     IFLDPRARAAQYDWESVARFVLSAFRVDAARAGAAAEVEPLINELCRLSSEFEAMWRD
                     NDVRGAHHGEAVKHIRHPTAGPLAFEYSAFSVDGRTDLAMVVYNPATPADADKIRALL
                     ESR"
     gene            5826..>6013
                     /locus_tag="VGC69_04850"
     CDS             5826..>6013
                     /locus_tag="VGC69_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY0294649.1"
                     /translation="MRIFITGATGFIGSAVVKELISAGHQVLGLCRSEAKAAALAAAG
                     AEVHRGSLEDPDSLKAGAA"
BASE COUNT         1035 a         2123 c         1872 g          983 t
ORIGIN      
        1 cggccagctg gccgcctgga actacttcga atccatcaag aacccggtca acgccgaatt
       61 cgtgaagaag tggaaggcct acgccaaggc ccagaacctg cccaactacc agacggccgt
      121 caccaacgac ccgatggaag ccacctacgt cggcatccac atgtggaagc aggccgtcga
      181 aaaggccaag agcaccgacg tcgaaaaggt gatcgcggcc atgggcggcc agaccttcaa
      241 ggcgccggac ggcttcacca tcgaaatgga caagactaac caccacctgc acaagcccgt
      301 ctatatcggc gaagtgcagg ccgacggcca gttcaaggtg gtgtggaaaa ccaagggccc
      361 catccgcgcg cagccgtgga gcccgttcat ccccggcaac gaaagcaagc aaaacatgta
      421 atcggatcaa gggtggtcat gcatcacgca gtgtttttcg caaggctgcg ccgcctgctg
      481 ctgggctcgc tgctcggcct ggccgccctg tccgcctgcg cgtacgcgca ggacgcggcg
      541 ccggcggcgt cgcaagacgc cgccacgccg ccatcccagg cccagggcct ggacgaatcg
      601 cttctgtcgc tgctggccag cgacgacaat gacgaaaaga tcgaggccat caacaagctg
      661 agccgcttgc ccgatccgca tgccgtggcg ttgctgcagg ccctggcgaa cgaccatgtc
      721 tatgccacct cggacaacaa ggtctatatc agcgacgacg acgggcagac cgcgcaggac
      781 cccatcacgg ggcagacgca acccatgccc gacggctccg acacgctcac cctcaacaac
      841 cgcgtgcgca gcgccatcga tgccgcgcag gccacggcca agctctattc gcccaaggtg
      901 gccgaccggc tggccgcggc ccagctgctg ctgcagtccg gcgacgaatc gctgctgccc
      961 atcgtagacc aggccctcaa gaaagaaacc gacgccggcg tgcgcacggc cctgcagacc
     1021 atccaggcgc acctgcagct ctcgtccaag gacccggccg cgcgccgcgc cgccgcgcag
     1081 accctgggcg aaagcggcaa cgcctcgttc cggccgatgc tgcaggccct ggttcaaccc
     1141 aagcccgacg gcagctacga cgaacccgat gcctcggtgc gcaatgccgc caccgccgcg
     1201 ctcaagcgca tcgaccagca cgcgacggtg atcgaatggg ccggcaacat ctactacggc
     1261 ctgagcctgg gcagcgtgct gctgctggcc gccctgggcc tgtccatcac cttcggcctg
     1321 atgggcatca tcaatatggc gcacggcgag ctgctcatga tcggcgccta cgccacctac
     1381 accgtgcagt ccgtcttccg gcaatggctg ccgggcctga tcgactggta cgtggtggcg
     1441 gccctgccgg tggccttcat cgtcacggcc ctggtgggca tggcgctgga gcgcaccgtg
     1501 atccgctggc tgtacggccg gccgctcgaa accctgctgg ccacctgggg catcagcctc
     1561 atcctcatgc aggccgtgcg ctcgatattc ggcgcgcaga acgtcgaagt ggccaacccc
     1621 agctggatgt ccggcgggct gaccgtgatg gacggcctgg tgctcaccta caaccgcctg
     1681 gtcatcatcg cgttcgccct gctggtggtg ttcttcgtct ggctgctgct caaccacacc
     1741 cggctgggcc tgttcgtgcg ggccatcacg cagaaccgca ccatggccga ctgcgtgggc
     1801 gtgcccaccg gccgcatcga catgctggcc ttcggcctgg gctcgggcat cgccggcctg
     1861 gccggcgtgg cgctgtcgca attgggcaat gtgggcccgg ccctgggcca gagctatatc
     1921 gtcgactcct tcatggtggt ggtgctgggt ggcgtgggcc agctggccgg caccgtgatc
     1981 gccggcctgg gcctgggctg gatcaacaaa ttcctcgaac cctacgccgg cgccgtcatg
     2041 gccaagatca tcatcctcgt cctcatcgtg ctgatcgtcc agaaacgtcc acaaggcctg
     2101 ttcgccccca aaggccggag cgctgaatga ccacccccac gctcattaca ttcgctgccc
     2161 cccaaggggg ctccggcctc cttcgggcgg ccgggcggag gccggaatga ccacagcctc
     2221 cgacctgatg cacctcaatc gccgtccgct gttcccgccc aaggtttggg cggtgcttgt
     2281 cgtggccatc gtcgtcatgg ccctgctgcc ggcgctcaat ctggccttcc cggacggcca
     2341 tccgctgcac atctcgtcct acaccatcgc ggtgggcggg cgcttcatgt gctatgccat
     2401 ggccgcgctg gcgctcgacc tggtgtgggg ctatgccggc atcctgtcgc tgggccacgg
     2461 cctcttcttc gccctgggcg gctatgcgca cggcatgtac ctgatgcgcg ccatcggcca
     2521 cgacggcgtc taccagagcg acctgcccga cttcatggtg ttcctcaact ggaaggccta
     2581 cccctggtac tggaccttca ccgaccactt cctgtacgcc atgctgctgg tggtgctggt
     2641 gcccggcgtg ctggccttcg tgttcggcta cttcgccttc cgctcgcgca tcaagggcgt
     2701 gtacttctcc atcatcaccc aggcgctcac ctacgccgcc atgctgctgt tcttccgcaa
     2761 cgacacgggc ttcggcggca acaacggctt caccgatttc aagcgcatcc tgggcttcaa
     2821 catcaccgag tccaccacgc gcgccgcgct cttctggatc acgctggccg tcctggtgct
     2881 ggcactcatc gtggcccgcc tgatcacgca gtccaagctg ggccgggtgc tgaccggcgt
     2941 gcgcgacgcc gaaagccgcc tgcgcttcct gggctacgag ccgctgggct tcaagctctt
     3001 cgtctggacg ctctcggccg tgatctgcgg catcgcgggc gccttgtacg tgccgcagat
     3061 cggcatcatc aaccccggcg aaatgtccac ggccaactcc atcgagatgg tgatctgggt
     3121 ggccaccggc gggcgcggca cgctgatcgg ccccatcatc ggcgccggcg tggtcaacag
     3181 cctcaagatc tggttcacca gcgcgtttcc cgactactgg ctctacgcgc tgggcctggt
     3241 gttcgtgctg gtcaccctct tcctgccgca gggcatcgtc ggcctggtgc gccagtgcgc
     3301 cgcgcgggtc ggcaaaatgc aggggggcaa caagcaatga gcaccgacaa tcgccccaac
     3361 cccggcggcc tggacaccag ccacggcgcc atcctctacc tggaagacat caccgtcagc
     3421 ttcgacggct tcaaggcgct gaacaagctc acgctggaca tcggcgtggg cgagctgcgc
     3481 tgcatcatcg gcccgaacgg cgccggcaag acgaccatga tggacgtcat caccggcaag
     3541 acgcgcccca cctcgggcag cgccttcttc ggccagacca tcgacctcac ctcgctttcc
     3601 gaggcggaaa tcgcccatga aggcataggc cgcaagttcc agcgcccgac ggtgttcgaa
     3661 aaccacagcg tgttcgaaaa cctcgagctc gccatgaaga ccgacaagcg cgtgcggccc
     3721 acgctcttct cgcgcctgtc ttccgaacag gccgaccgca tcgcgcagac gctggacctc
     3781 atccgcctgg ggccgctggc cggcgaagcc gccgggctgc tgtcgcacgg ccagaagcaa
     3841 tggctggaga tcggcatgct gctcatgcag gaacccaggc tgctgctgct cgacgagccc
     3901 gtggccggca tgaccgacgc cgagaccgag cgcaccggcg agctgctcaa cgaactgcgc
     3961 ggcaagcatt cgctgatggt ggtcgagcac gacatggatt tcgtcacgca gatcgcgggc
     4021 ggcggcaagg tgaccgtgct gcacgagggc tcggtgctgg ccgaaggccc catggcgacg
     4081 gtgcaggccg accctcgcgt catcgaagtc tatctgggcc gctgaacggg accccaccat
     4141 gctggacata aaagacatca accagtatta cggcggcagc cacaccttgc gcagcatctc
     4201 gctgtcgctc gcgcgcggcg agtgcctggc gctgctgggc cgcaacggcg tgggcaagac
     4261 cacgctgctc aagtgcctga tgggcgtgct gcccacggcc agcggcgcca tcagcctgga
     4321 cggcgccgac atcaccaggc ttgccccgca ccagcgcgcc gccctcggca tggcctacgt
     4381 gccccaaggc cgggaaatct tcgcgcgcct gacggtcgag gaaaacatcg tcatgggcat
     4441 ggccaccaag ccgtcctcgc aagcccgccg catcaagggc gagatcttcg agctctttcc
     4501 cgtgctcaag accatgctcg gccggcgcgg cggcgacctg tccggcggcc agcagcagca
     4561 gctggccatc gcccgggcgc tggtggccga gcccaagctc atcatcctcg acgagcccac
     4621 cgagggcatc cagccgtcca tcatcaagga catcggccgc gtcatccaca tgctgcgcca
     4681 gcgcggcgac atcggcatcc tgctgtgcga acagtatttc gacttcgccc gcgagctggc
     4741 cgaccagttc gtcgtgctct cgcgcggcga agtcgtggcc cggggcgacc gcagccagat
     4801 cgacagcgag gacgtgcgca ggcacctgtc ggtgtagacg gcagcgggct cccagggccg
     4861 gcccctggcc tcccggccaa gatgcctata ccgtcatcgg gattcgagca gcgctctgat
     4921 cttgtccgca tccgccggcg tcgccgggtt atagaccacc atggcgaggt ctgtccggcc
     4981 gtcgactgaa aaggccgaat attcgaaggc gagcgggccg gccgtcgggt ggcgtatgtg
     5041 cttgacggcc tcgccatgat gggcgccacg gacgtcgttg tcgcgccaca tcgcctcgaa
     5101 ctcggaactg agccggcaaa gttcgttgat gagcggctcc acctccgccg cggcgcccgc
     5161 gcgcgccgca tccacccgga acgcgctcaa cacgaaccgc gccacgcttt cccaatcgta
     5221 ttgggcggcc cgggcacgcg ggtcgaggaa aatgaagcgc aggatattgc ggtgctccgg
     5281 cggcaacgat tcgtaatcca tcaacatcac ggtcgacgcc cggttccagg cgacgacatc
     5341 ccacgtcgcc gtcctgatca gggcggggct ggggttcaag gcatccagaa cgcgctgcag
     5401 ccggggcgtg acgctttcgt tcttccggta gcgcgcctcg ggcgggcggc cgaggccaag
     5461 caggaaaagg tgctcgcgct cgacatcggt cagcatcagg gcgcgggcga tccggtcaag
     5521 cacgtcggcc gagggcgcgc caccccgccc ctgctcgagc catgtgtacc aggtcgggct
     5581 gatattggcg cgctgcgcca cctcttcgcg gcgcaaaccg ggcgtgcgcc gccgcgccgg
     5641 aggaaagcca aacgccgccg gatcgagctg cgtgcggcgg tgcttcagat agtccccgag
     5701 cagattcccg tccgcggcgg cctgagcacg catcctgtta atccctatac catgatgatc
     5761 taactacttt acggggatag tttaaaggta gagattgtct ccagcgaaac ctggagactt
     5821 tgttcatgcg tatattcatc accggcgcca ccggtttcat cggttcggcc gtcgtcaagg
     5881 agttgatctc ggcaggccat caggtgctcg gcctctgccg ctcggaggcc aaggcggcgg
     5941 ccctcgcggc cgcaggcgcc gaggtccatc gcggatcgct cgaggatccg gacagcctca
     6001 aggcgggcgc cgc
//
LOCUS       DASWPO010000082         4698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2250239, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000082 DASWPO010000000
VERSION     DASWPO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250239"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VGC69_04855"
     CDS             <1..279
                     /locus_tag="VGC69_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294650.1"
                     /translation="YGDTKIKDKPRNFIGMSKSLPPDQMNALLRTAAPMDRQALRDLA
                     DARAKAVYELLLSTAQALTDRVFIVAPKLDADGIEGGGKATRVDFALQ"
     gene            333..1217
                     /locus_tag="VGC69_04860"
     CDS             333..1217
                     /locus_tag="VGC69_04860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0294651.1"
                     /translation="MQDINDPEFDSLLPPLRLSRRGFIATSLGAGFTLATGHAAAQTA
                     IHTDATGLTAGPVEIPTADGRMPAYRAAPAGKKNPPVILVVEEIFGVHEYVQDVCRRL
                     AHLGYLAVAPELFARYGDPAKYTDMASLRAEVVDKAADSVVISDLDATAQWALAQGGS
                     AEKMGIIGFCWGGRQVWLYTMHNPKLKAAAIFYGPLAGAPTALQPASVLSQVDKVAVP
                     VLGAYGGKDAGISQDDIDKMRVALANGPAPAKASRIDVYPDAGHGFHADYRPSYNKAD
                     AELAWKRAMDWFADHGLN"
     gene            1324..1827
                     /locus_tag="VGC69_04865"
     CDS             1324..1827
                     /locus_tag="VGC69_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0294652.1"
                     /translation="MTIKVGDTLPDGTLTEFIEVETEACTLGPNAFQVADLVKGKKIA
                     IFAVPGAFTPTCSAKHLPGYVAHADALFAKGVDEIWCVAVNDAFVMGAWGRDQKVAGR
                     VRMMADGAATWTRALGLDMDLTARGMGVRSQRYSALVENGVVKKLNVEAAGKFEVSDA
                     DTLLSQL"
     gene            1942..3057
                     /locus_tag="VGC69_04870"
                     /pseudo
     CDS             1942..3057
                     /locus_tag="VGC69_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3009..4064)
                     /gene="bioB"
                     /locus_tag="VGC69_04875"
     CDS             complement(3009..4064)
                     /gene="bioB"
                     /locus_tag="VGC69_04875"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY0294653.1"
                     /translation="MNHTAPQAGTWQVKDLLALYELPLPELIYRAQAAHRENFPADTV
                     QLSTLLSIKTGGCPEDCAYCPQAARHDTGVQAEALLPLADVLAAARAAKDNGAQRFCM
                     GAAWRSPKPHQVDAVAEMVAGVKALGLETCVTLGMLREGQAAQLKAAGLDYYNHNLDT
                     APEFYGNVISTRTYQDRLDTLERVRDAGISVCCGGIVGMGETRRQRAGLIAQLAGLHP
                     YPESVPINNLVRVAGTPLAEAEPLDVFEFVRTIAVARIAMPRAMVRLSAGREALNDAE
                     QALCFMAGANSIFYGEALLTTANPQAQADQLLLARLGLRAQGGARTGEDQDPAAGPGS
                     FDRTRSTSSSAHAETNM"
     gene            complement(4122..>4698)
                     /locus_tag="VGC69_04880"
     CDS             complement(4122..>4698)
                     /locus_tag="VGC69_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HEY0294654.1"
                     /translation="AQRLSLFIPLLAAFALFGEALTQRKGLAMLAACAALACLLNRPG
                     AAGKGEGGAGQGWLWPLSVWVGYGVIDILFKRLSQTGTAFASGLLGIFVLAAVLMLLY
                     LLFARAQWQWRTLATGLVLGAVNFANIYTYIKAHQSLPGNPALVFAAMNMGVVALGTL
                     IGAMAFREPLSRVNLLGLILALAAIAAMMPT"
BASE COUNT          707 a         1623 c         1585 g          783 t
ORIGIN      
        1 tacggcgaca ccaagatcaa ggacaagccg cgcaacttca tcggcatgtc caagtcgctg
       61 ccgcccgacc agatgaacgc gctgctgcgc acggccgcgc cgatggacag gcaggccctg
      121 cgcgacctgg ccgacgcgcg cgcgaaagcg gtgtacgaac tgctgctgtc gacggcgcag
      181 gccttgaccg accgcgtctt catcgtggcg cccaagctcg atgccgatgg catcgagggc
      241 ggcggcaagg ccacgcgcgt cgacttcgcc ttgcagtaac ccgatgcaga cgggtacatg
      301 cctcatgcag atcaacccag ggagctcccg ccatgcaaga catcaatgat cctgaattcg
      361 acagcctgct gccgccgctg cggctgtcgc gccgcggttt catcgccacc agcctgggcg
      421 cgggctttac cctggccacc ggccatgccg cggcgcagac ggccatccac accgacgcga
      481 ccggcctgac ggccggcccc gtggagatcc ccacggccga cggccgcatg ccggcctatc
      541 gcgcggcgcc ggccggcaag aagaacccgc cggtgatcct ggtggtcgag gaaatattcg
      601 gcgtgcacga atacgtccag gacgtctgcc gccgcctggc gcacctgggc tacctggccg
      661 ttgcccccga actgttcgcc cgctacggcg acccggccaa gtacaccgac atggcctccc
      721 tgcgcgccga ggtggtggac aaggccgccg actcggtggt catctccgac ctcgatgcca
      781 cggcgcaatg ggcgctggcc cagggcggct cggccgagaa gatgggcatc atcggcttct
      841 gctggggcgg ccgccaggtt tggctataca ccatgcacaa ccccaagctc aaggccgccg
      901 cgatctttta cggacccctg gccggcgcgc ccaccgcgct gcagccggcc tcggtgctca
      961 gccaggtcga caaggtggcc gtgccggtat tgggcgccta cggcggcaag gacgccggca
     1021 tttcgcagga cgacatcgac aagatgcgcg tggccctggc caatgggccg gcgccggcca
     1081 aggcgtcgcg catcgatgtc tatcccgatg ccggccacgg ttttcatgcc gattaccggc
     1141 ccagctacaa caaggccgat gccgaattgg cctggaagcg cgccatggac tggttcgccg
     1201 accacggcct gaattgatcg cagcggcgca agcgagccgg tctatgatga cgggttcgcg
     1261 cagccgctgc gccacacgca ttccacaccg agtttcacac cgtatttcat actggagaga
     1321 accatgacga tcaaagtcgg cgacaccctg cccgatggca ccctgaccga attcatcgaa
     1381 gtcgaaaccg aggcctgcac cctggggccc aatgccttcc aggtggccga cctggtcaag
     1441 ggcaagaaaa tcgccatctt cgccgtgccg ggcgccttca cccccacctg ctcggccaag
     1501 cacctgccgg gctatgtcgc ccacgccgac gccctgttcg ccaagggcgt ggacgagatc
     1561 tggtgcgtgg ccgtcaacga tgccttcgtc atgggcgcct ggggccgcga ccagaaggtc
     1621 gccggccgcg tgcgcatgat ggccgacggc gccgccacct ggacccgcgc cctgggcctg
     1681 gacatggacc tcaccgcgcg cggcatgggc gtgcgctcgc agcgctactc ggccctggtc
     1741 gagaacggcg tggtgaaaaa gctcaatgtc gaagccgctg gcaagttcga ggtcagcgat
     1801 gccgacaccc tgctgtcgca gctttgatct tgtttgactt ttgctgactc gccgcccgcg
     1861 tagccttgca ggctacgcgg gcgttttgct ttggccgtcc gccgtttccc attgttcgct
     1921 gtccccgcca cgccggtctt catgcttgcc acgctcgcct cgttcacgcc gctctacctg
     1981 accaccttgc tgctgctggg cggcacgggt ctgatcaata tgtacatggg cctgtacctg
     2041 gcccagcaat cggtcagcga aacctggatc ggcaccctca tggcgggcta ttacctgggc
     2101 ctggtgctgg gcagccgctt cgggcataag ctcatcagca aggtggggca catccgcgct
     2161 ttctcggcct gcgcggcggt gggcacctgc atcgtgctgg ggcagggcct gtccgactgg
     2221 ctgcccttgt ggctggcgct gcgcatcgtc ttcggcgtca tgctcgtgac ggcgctcgtg
     2281 gtcatcgaga gctggctcaa cgagcagacc accaaccagc agcgtggccg cgtgttctcg
     2341 gtgtacatgg tggtgtctag cctgggcacc atgctgggcc agctggcgct ggctcgctat
     2401 acccggctca acgacgagcc cctgaccatc gcggccatgc tgtgcgccct gtgcctggtg
     2461 ccgctgaccg tggtcaacgg cgtgcatccc gccacgccgc ggccggcgcc gctggacatc
     2521 ccgtactacc tgcggcgcgt gcccgcggcg cttttcgtgc tgttcatctc gggcctggtg
     2581 ctgggctcgt tctacggcct ggcggccgtc tatgtcgtca agcagggcct ctcgcccgcc
     2641 caggccgtgg tcttcacgtc ggtggccgtc ggctgcgcgc tcgtgtcgca atggcccatg
     2701 ggctggctgt cggaccgtgt cgggcgggat cgcctcgtct atctcaacgc cctgttgctg
     2761 gccgtgttgc cggtgctgct gtggggctgg ttcgcgccgc ccttctggct gctgctggtg
     2821 tttgcctgcg tgcagggacc gctgcagtcc agcctctatc ccctgggcgt ggccctggcc
     2881 aacgaccgca tcgaccccga ccgccgcgtg ggcgtgagcg ccgtcgtgta catggcctat
     2941 ggcgtcggcg cctgcgtcgg cccgctggtg gccggggcgc tgatgtcgca cgtcgggccc
     3001 tcggcctact acatgttcgt ttcggcgtgc gcgctgctgc tggtgctgcg ggtccggtcg
     3061 aaggagcctg ggccggcggc cgggtcctga tcctcgccgg tccgcgcgcc gccttgcgcg
     3121 cgcaggccca ggcgggccag cagcagttgg tcggcctgcg cctgcggatt ggcggtcgtg
     3181 agcagcgcct cgccgtagaa gatggaattg gcgccggcca tgaagcacag ggcctgctcc
     3241 gcatcgttca atgcctcgcg gccggcggac agtcgcacca tggcgcgcgg catggcgatg
     3301 cgcgcgacgg cgatggtgcg gacgaactcg aagacgtcca gcggctcggc ctcggccagc
     3361 ggcgtgcctg cgacgcgcac caggttgttg atgggcacgg attcagggta ggggtgcagg
     3421 ccggccagct gcgcgatcag gccggcgcgc tgccggcgcg tttcgcccat gccgacgatg
     3481 ccgccgcagc agaccgagat cccggcgtcc cgcacgcgct ccagcgtgtc gaggcggtcc
     3541 tggtaggtgc gcgtggaaat gacgttgccg tagaactcgg gcgcggtatc caggttgtgg
     3601 ttgtagtagt ccaggccggc cgctttcaat tgcgccgcct ggccttcgcg cagcatgccc
     3661 agcgtgacgc aggtctcaag ccccagcgcc ttgacgcccg ccaccatctc ggccacggcg
     3721 tcgacctggt gcggcttggg gctgcgccag gcggcgccca tgcagaagcg ttgcgcgccg
     3781 ttgtccttgg cggcccgggc agcggccagc acgtcggcca ggggcagcag ggcctcggcc
     3841 tgcacgcccg tgtcatggcg cgcagcctgc gggcaatagg cgcagtcctc ggggcagccg
     3901 cccgtcttga tcgacaggag cgtcgagagc tgcacggtgt cggccgggaa attttcccgg
     3961 tgcgccgcct gggcccgata gatcagttcg ggcaagggca gttcgtacag ggcgagcaga
     4021 tccttgacct gccaagtacc agcctggggc gcggtgtgat tcatggtgtg tgccgggaaa
     4081 gagggaaggg agcagccggt ggccggccgc cagcgatgga ttcaggtggg catcatggcg
     4141 gcaatcgccg ccagcgccag gatcagcccc agcaggttca cgcggctgag cggctcgcga
     4201 aacgccatgg cgccgatcag cgtgcccagc gccacgacgc ccatattcat ggcggcgaac
     4261 accagcgccg ggttgccggg caggctctgg tgcgccttga tataggtata gatgttggcg
     4321 aagttcaccg cgcccagcac cagccccgtc gccagggtgc gccactgcca ttgcgcgcgg
     4381 gcaaacagca ggtacagcag catcagcacc gccgccagca cgaaaatgcc cagcagcccg
     4441 ctggcgaagg ccgtgccggt ttgcgacagg cgcttgaaaa ggatgtcgat cacgccatag
     4501 cccacccata ccgacagcgg ccacagccaa ccctggcccg cgccgccttc gcccttgccg
     4561 gcggcgcccg ggcggttgag caggcaggcc agcgctgcgc aggcggccag catggccagt
     4621 cccttgcgtt gcgtcagtgc ttcgccaaag agcgcgaagg ccgccagcag cgggatgaag
     4681 agcgaaagcc gctgcgcc
//
LOCUS       DASWPO010000083         9468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3143079, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000083 DASWPO010000000
VERSION     DASWPO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143079"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGC69_04885"
     CDS             complement(<1..435)
                     /locus_tag="VGC69_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0294655.1"
                     /translation="MNTNTLDAPVAAPSVWLGPDMARNPATWIYQLSNAELQEIAAAV
                     SHARSIGLSIPTLAAEGFPLPHLAPKLRELLYEVQFGRGFVLIRGMGALNYGEEDLSL
                     AYWGLGAYFGKPATQNAKGDVLGHVRDEGRKFGELDARGYHSN"
     gene            849..1739
                     /locus_tag="VGC69_04890"
     CDS             849..1739
                     /locus_tag="VGC69_04890"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294656.1"
                     /translation="MDRRRLECFLALAEELHFHRAAVRCHMTQPALSQQLRVLEEQLQ
                     VQLVYRSKRHVALTRAGEVFLDEVRKILRGMDLAVAMARRTERGEIGQLKIGVTAPAL
                     YIVFPEIVRAFAQRLPEVGLIVHDMTTAEQEQALRNRSIQLGIVHPPLEDTALSCTLI
                     ATTAFNIVLSDQNPLAKRRRLRLRDLANEQFIIFPRTIGPQLYDHIISLCQSEGFSPK
                     VILEITPAQSIIAMAAAGFGIGFIASEYQLLPRPGVVFRKLEGVAPSLSLGVAYDGSD
                     VSPAMKVFLELAQRIGAQVR"
     gene            1879..3093
                     /locus_tag="VGC69_04895"
     CDS             1879..3093
                     /locus_tag="VGC69_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0294657.1"
                     /translation="MASWQEAVPQSAPAIKIVSIRTIVVNAEMRNWIFVKVQTDQDGL
                     YGWGEASLNWKTKAVTGAVEDLEPLLLGRDPRDIEQCVRAMNKHSYYKLGIIGATAIS
                     GIEHALWDIHAKSLGVPVWRLLGGKVRDKVRLYTHLGLGDMKSVYETFDHGKLRDSAL
                     AVVEKGYDALKVVFIPYGNHIASIPAQRHVGRLMETVRSAVGESVDIMVDFHGRPGSI
                     AAALGFIEELAPYRPMFCEEPVQPGDTEALRQVALRAPCPIASGERLVGLNEFMPVLT
                     GKALHVAQPDLNHTGGLLEGKRIASVAEAELIGIAPHNPNGPIAGVAALHYDVSTPNF
                     LIQEEMSGAVPWYDDVVQTPLKRTGSYWEVPHGPGLGVEINEKEAAKHPFKQEVMHAM
                     GAVLDDGTIVEW"
     gene            3113..4210
                     /locus_tag="VGC69_04900"
     CDS             3113..4210
                     /locus_tag="VGC69_04900"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HEY0294658.1"
                     /translation="MNPNQPNDTLRFATPGGIYNATPERIVFAAGAVDAIADEVARLG
                     IKRALVVTTPGRGELGQRIVDLLGERCAGLLPEAVSQVPIELARRGREAARAAGADGI
                     VSAGGGAAIGLGKGISLELGLPIVAVPTTYSGSEVTGFCGITIDGVKRMHTSRNMLAR
                     TVVYDPRLTLGLPVDVSAASAMNAMAHCVDGIYVPTVDPVTAMAAVEGARVVAEAAPR
                     MVETPDDLGARTALLYGAYLGGVALTGGFALQHGLAHVLGGSFGILHGPSHALVLPYV
                     AAYNSRYAPAPLRRLAEAIGARSLGAGLYDLARSLRVDMGMSGTGLSRQALDEAARIT
                     LETDDGDNPGPVTLEAVRGILEQAWRGERPD"
     gene            4284..5294
                     /locus_tag="VGC69_04905"
     CDS             4284..5294
                     /locus_tag="VGC69_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0294659.1"
                     /translation="MNITRRALIKAAGAAALGGALPAARAATAEYAWKFGHGFPPTHP
                     IHQRAVQAAAWIKQESKGQVDIGVFPNSQLGGDNDLLSQVYSGGVEMFTTGGVIMSTL
                     VPHAALSGLGFLFQDYPAVWTAMDGALGAFVRDAFAKKNLHAIDHIWDNGFRQITTST
                     RPIATPQDLANLKIRVPVSPVYISLFKALGAAPTSINLAEVYTALQTGVVEGQENPLV
                     VTDTAKFYEVQKYCSITNHIWDGSWIVINGDAWESLPADLQRIVSRGFNDLAPTQRDD
                     IAKLNQSLRDGLLGRGLQVNATNPAPFRDLLRQAGFYADWHNKFGDAAWRTLESAVGR
                     LT"
     gene            5357..7174
                     /locus_tag="VGC69_04910"
     CDS             5357..7174
                     /locus_tag="VGC69_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0294660.1"
                     /translation="MALQEACWRWLVEYPAAALVAAEVVILFTGIVARYALHMPITWT
                     DEAASILFLWLSMLGSVVALQRGEHMRMTALVSRCKPGTRDFLDAVAACASLAFLGLV
                     LVPAIHFAHKQDAFTTPALEISGAWRAMALGVGIALMLVACVLRLLRMPDRRLALGAL
                     AVVAAVSVGFWLLQPALAGLGKANLLIFFLGVVVLTVFAGVPIAFSFGLATFGYLALT
                     TRVPLETMVGRMDEGMSHLILLAVPLFIFLGLLIEMTGMAQRMVAFLANLIGHKRGGL
                     AYVLIGAMYLVSGISGAKAADMAAVAPALFPEMKRRGAKEGDLVALLAATAAQTETIP
                     PSIVLITIGSVTGVSIAALFTGGLLPGALLAAALCCVVWRRYRREDMSGAAWPGRGAI
                     WRSLRIALPALALPFVIRFAVVDGVATATEVSTIGIVYALVVGLLVYRCFDRSRLVPM
                     LVETAALSGAILLIIGTATAMAWCLTRSGFSNDLAALMAGLPGGKLGFLGVSILMFIV
                     LGSVLEGVPAVVLFGPLLFPIAQQMGVHEVHYAMVVILSMGIGLFAPPFGVGYYVAAA
                     IGKASPDAGMRPVAGYLLALFIGVLVIAAVPWISTGFLP"
     gene            7171..8172
                     /locus_tag="VGC69_04915"
     CDS             7171..8172
                     /locus_tag="VGC69_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY0294661.1"
                     /translation="MNGASEGQPAMKIFLVGEAATHADELVQHLQTPAQLVPLPREAA
                     HDAGHDALIGPQDVVVSLRFARRGEAPPFRLLHVPGAGLDGIDTACLPPGCAVCNVFE
                     HEIPIAEYVCLAMLEWQVRLRALTASFTAEAWPDLYRDRRPHGELHGKTLALIGLGRI
                     GRAIAQRAGAFGMRVLAVDGHAPQPPQGLVERLVAPHDWEEALEQADFVAITCPLTPQ
                     TRGLFNARSLRRMRRDAVLINVSRAEIADERALYEALRDRVIGGAVLDVWYRYPQGAG
                     DRVAPSDQPFLDLPNVIATPHSSAWTDGLPRRRYAFIARNIDRLVRGEPLQNRVRAP"
     gene            8337..8585
                     /locus_tag="VGC69_04920"
     CDS             8337..8585
                     /locus_tag="VGC69_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0294662.1"
                     /translation="MSNLARTPQQIGALIRRNRKQLGLSQSALGERVGLRQETISLIE
                     TGNPATRLDTLLAVLAALDLELQIAARTRQHDVADLIE"
     gene            8585..>9468
                     /locus_tag="VGC69_04925"
     CDS             8585..>9468
                     /locus_tag="VGC69_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEY0294663.1"
                     /translation="MARTRRHPPLRVYQNNRLVGHLLKSPNGAIEFRYDATWLDRSTA
                     FPVSLSLPLQEAPWRGEPVVAVFENLLPDSEVLRRRVAERVGAAGSDAYSLLAAIGRD
                     CVGALQFVAGDAAEPDTNAGISGEPVDGEAIERLLKSLGQAPLGLSRDDDFRISVAGA
                     QEKTALLQVDGQWFKPHGTTPTTHLFKTPIGKLPNGIDLSNSVENEFYCLRLLSHLGL
                     PVNHAEIMTFGQTKALVIERFDRAWRNGRLIRLPQEDCCQALSVPPARKYQSDGGPGM
                     LDIANLLKGSDSPADDHRT"
BASE COUNT         1482 a         3350 c         3137 g         1499 t
ORIGIN      
        1 attgctgtga tagccgcgcg cgtccagctc gccgaatttc cggccttcgt cgcggacgtg
       61 gcccagcacg tcgcccttgg cgttttgcgt ggccggcttg ccgaagtagg cgcccagccc
      121 ccagtacgcc aggctgagat cctcctcgcc gtagttcagc gcgcccatcc cgcggatcag
      181 gacaaaccct cgtccgaact gcacttcgta tagcagctcg cgcaacttgg gcgcaagatg
      241 cggaagcggg aatccctcgg ccgccagggt cgggatcgac agcccgatgc tcctggcatg
      301 ggagacggcg gcggcgatct cctggagttc ggcgttagac agctggtata tccatgttgc
      361 ggggttccgc gccatatcgg ggcccagcca gaccgagggg gccgccacag gggcgtctag
      421 ggtgtttgta ttcatgacat ctacctatac ggaagcggaa agacgctttg ttatgccaaa
      481 gtgtcaaaca gaaatacaag agtgatctga ttaaatacca attcctggag gaatgctagt
      541 gtgatctccc ccgggtcatg agggcgcacc cgcgccaggg ctggaagggc ggggaaaggt
      601 cgccctcgag ggccacggac ctctccggga gtccgcatac aatggccggc cacgctgcgc
      661 tggcgctcgc accgcgcgac agggcagcga tcaccccgcg gggagaccgc catgcgaccg
      721 gacatcgaca cacacgccgc catcaccgcc gccggcagcc cgggcgcttc cggcatcgcc
      781 gcgcgcccgg tccgcgccgc gcgggcggcc tagcccctcc gcgcccgcag ccccgacgac
      841 cgccccgcat ggaccgccgc cgactcgaat gcttcctcgc cctggcggaa gaactgcact
      901 tccaccgcgc ggcggtgcgc tgccacatga cccagcccgc gctcagccag cagttgcgcg
      961 tgctcgagga gcagctgcag gtgcagctgg tgtaccgcag caagcgccac gtcgcgctca
     1021 cgcgcgccgg cgaggtcttc ctcgacgagg tgcgcaagat cctgcgcggc atggacctgg
     1081 ccgtggccat ggcgcgccgc accgagcgcg gcgagatcgg ccagctcaag atcggcgtga
     1141 ccgccccggc gctgtacatc gtgttcccgg aaatcgtgcg cgccttcgcg cagcgcctgc
     1201 ccgaggtcgg cctgatcgtg cacgacatga ccaccgccga gcaggaacag gcgctgcgca
     1261 accgcagcat ccagctcggc atcgtccatc cgccgctcga ggacaccgcg ctgtcgtgca
     1321 cgctgatcgc caccacggcg ttcaacatcg tgctgtccga ccagaacccg ctggccaagc
     1381 ggcgccggct gcggctgcgc gacctggcca atgaacagtt catcatcttc ccgcgcacca
     1441 tcgggccgca gctctacgac cacatcatca gcctctgcca gagcgagggc ttcagcccca
     1501 aggtgatcct ggagatcacg ccggcgcagt cgatcatcgc catggccgcg gccggcttcg
     1561 gcatcggctt catcgcgtcc gagtaccagc tgctgcccag gccgggcgtg gtgtttcgca
     1621 agctcgaggg cgtcgcgccc tcgctgtcgc tgggcgtggc ctacgacggc agcgacgttt
     1681 cccccgcgat gaaggtgttc ctggagctgg cgcagcgcat cggcgcccag gtgcgctgag
     1741 cccgcgcgag gctgccggac ggcctcggtc cttgataaga aaaagttctc aaacgagtaa
     1801 aaaaaagaat tagaagtttt tcttcgccaa atctattgtt gtctccatcc acacaaaaaa
     1861 cacgattgga gacaagcaat ggcctcatgg caagaggcgg tgccgcagtc cgcgcccgcc
     1921 atcaagatcg tcagcatccg cacgatcgtc gtcaacgccg agatgcgcaa ctggatcttc
     1981 gtcaaggtgc agaccgacca ggacggcctg tacggatggg gcgaggccag cctgaactgg
     2041 aagaccaagg ccgtgaccgg cgcggtggag gacctggaac ccctgctgct gggccgcgac
     2101 ccgcgcgaca tcgagcaatg cgtgcgcgcg atgaacaagc acagctacta caagctgggc
     2161 atcatcggcg ccaccgccat cagcggcatc gagcacgccc tgtgggatat ccatgccaag
     2221 agcctgggcg tgcccgtctg gcgcctgctg ggcggcaagg tgcgcgacaa ggtgcggctg
     2281 tacacgcacc ttggcctggg cgacatgaag tccgtgtacg aaaccttcga ccacggcaag
     2341 ctgcgcgaca gcgcgctggc ggtggtggag aagggctacg acgcgctcaa ggtcgttttc
     2401 atcccctacg gcaaccacat cgcctcgatc cccgcgcagc ggcatgtcgg ccggctgatg
     2461 gaaaccgtgc gcagcgcggt gggcgagagc gtggacatca tggtcgactt ccacggccgg
     2521 cccggctcga tcgccgccgc gctgggcttc atcgaggagc tggcgcccta ccggccgatg
     2581 ttttgcgagg agccggtgca gcccggcgat acggaggcgc tgcggcaggt cgcgctgcgg
     2641 gcgccctgcc cgatcgcctc cggcgagcgc ctggtgggcc tgaacgaatt catgcccgtg
     2701 ctcacaggca aggcgctgca cgtggcccag cccgacctca accacaccgg cggcctgctc
     2761 gaaggcaagc gcatcgcctc ggtggccgag gccgagttga tcggcatcgc gccgcacaac
     2821 cccaacggcc ccatcgccgg ggtggccgcg ctgcactacg acgtctccac gcccaatttc
     2881 ctcatccagg aagagatgag cggcgcggtg ccctggtacg acgacgtggt gcagactccg
     2941 ctcaagcgca ccggctccta ctgggaagtg ccgcatgggc cggggctggg cgtggagatc
     3001 aacgagaagg aggccgccaa gcaccccttc aagcaggaag tcatgcatgc gatgggcgcc
     3061 gtcctcgacg acggcaccat cgtcgaatgg taagcccccg gaacccgaca cgatgaatcc
     3121 gaaccaaccg aacgacacgc tccgcttcgc cacccccggc ggcatctaca acgccacgcc
     3181 cgagcgcatc gtcttcgccg cgggcgccgt ggacgccatc gccgacgagg tcgcccgcct
     3241 gggcatcaag cgcgcgctcg tcgtcaccac gcccggccgc ggcgagctgg gccaacgcat
     3301 cgtcgacctg ctgggcgagc gctgcgccgg cctgctgccg gaagccgtct cgcaagtgcc
     3361 catcgaactg gcccgccgcg gccgcgaggc ggcgcgcgcc gccggcgccg acggcattgt
     3421 cagcgccggc ggcggcgcgg ccatcggcct gggcaagggc atctcgctgg aactggggct
     3481 gcccatcgtg gccgttccca ccacctattc cggctcggaa gtcaccggct tctgcggcat
     3541 caccatcgac ggcgtcaagc gcatgcacac cagccgcaac atgctggccc gcaccgtcgt
     3601 ctacgacccg cgcctgacgc tcggcctgcc cgtggacgtg agcgccgcca gcgcgatgaa
     3661 tgccatggcg cactgcgtcg acgggatcta cgtgccgacc gtcgacccgg tcacggcgat
     3721 ggccgcggtc gagggcgcgc gcgtggtcgc cgaggcggcg ccgcgcatgg tggagacccc
     3781 cgacgacctg ggcgcgcgca ccgcgctgct gtacggcgcc tacctgggcg gcgtcgcgct
     3841 caccggcggc ttcgcgctgc aacacgggct ggcccacgtg ctggggggca gcttcggcat
     3901 cctgcacggc ccctcgcacg cgctggtgct gccctacgtg gcggcctaca acagccgcta
     3961 cgcgcccgcc cccctgcggc gcctggccga ggccatcggc gcccgcagcc tgggcgccgg
     4021 gctgtacgat ctggcacgat cgctgcgcgt ggacatgggc atgtccggca ccggactgtc
     4081 ccggcaggcg ctcgacgagg ccgcgcgcat caccctggag accgacgacg gcgacaatcc
     4141 gggtccggtc acgctcgagg ccgtgcgcgg catcctcgaa caggcctggc gaggcgagcg
     4201 gcccgactga ccccgggacg gccgccggcc gttccccgca tgacaaacga gagccggaca
     4261 cggccaagac cacggagaca accatgaata tcacccgcag agccttgatc aaggcggccg
     4321 gggccgcggc gctgggcggc gccctgcccg cggcgcgcgc cgccaccgcc gagtacgcct
     4381 ggaagttcgg ccacggcttc ccgcccaccc atccgatcca ccagcgcgcc gtgcaggcag
     4441 ccgcctggat caagcaggaa agcaaggggc aggtcgacat cggcgtgttc cccaacagcc
     4501 agctcggcgg cgacaacgac ctgctctcgc aggtgtactc gggcggcgtc gagatgttca
     4561 ccaccggcgg cgtgatcatg tccacgctgg tgccgcacgc ggcgttgagc ggcctgggct
     4621 tcctgttcca ggactatccg gcggtctgga cggccatgga cggcgcgctc ggcgccttcg
     4681 tgcgcgacgc cttcgccaag aagaacctgc acgcgatcga ccacatctgg gacaacggct
     4741 ttcgccagat caccaccagc acccgcccga tcgccacgcc gcaggacctg gccaacctca
     4801 agatccgggt gccggtcagc ccggtgtaca tctcgctgtt caaggcgctg ggcgccgccc
     4861 ccaccagcat caacctggcc gaggtctaca ccgccctgca gaccggcgtg gtcgagggcc
     4921 aggaaaaccc gctggtggtc accgacacgg cgaagttcta cgaagtgcag aagtactgct
     4981 ccatcaccaa ccacatctgg gacgggtcgt ggatcgtgat caacggcgac gcctgggaat
     5041 ccctgccggc cgacctgcag cggatcgtca gccgcggctt caacgacctc gcccccacgc
     5101 agcgcgacga catcgccaag ctcaaccagt cgctgcgcga cgggctgctt ggccggggcc
     5161 tgcaggtcaa cgccacgaac cccgcgccct ttcgcgacct cctgcgccag gcgggcttct
     5221 atgccgactg gcacaacaaa ttcggcgacg cggcctggcg cacgctcgaa tccgcggtcg
     5281 ggaggctgac gtgagcaccg cgcggctcgc gccgcccgat gcgcgccatg gcgggcagcg
     5341 gcgcgcatcg ggcggcctgg ccctgcagga ggcctgctgg cgctggctgg tggagtatcc
     5401 ggccgccgcg ctggtggcgg ccgaggtggt catcctcttc accggcatcg tcgcgcgcta
     5461 cgcgctgcac atgcccatta cctggacgga cgaggccgcc tcgatcctgt tcctgtggct
     5521 gtccatgctg gggtcggtcg tagcgctgca acgcggcgag cacatgcgca tgaccgcgct
     5581 ggtctcccgc tgcaagccgg gcacgcgcga cttcctggac gccgtggcgg cctgcgcctc
     5641 gctggccttc ctggggctgg tgctggtgcc cgcgatccat ttcgcccaca agcaggacgc
     5701 cttcaccacc ccggccctgg aaatctcggg cgcctggcgc gccatggcgc tgggggtcgg
     5761 catcgcgctg atgctggtcg cctgcgtcct gcggctgctg cgcatgccgg accggcggct
     5821 ggcgctgggc gcgctcgccg tggtggccgc cgtctcggtg ggcttctggc tgctgcagcc
     5881 ggcgctggcc ggcctgggca aggccaacct gctgatcttc ttcctgggcg tggtggtgct
     5941 gacggtgttc gccggcgtgc cgatcgcctt ctcgttcggg ctggccacct tcggctacct
     6001 ggccctgacc acgcgcgtgc cgctggagac catggtgggc cggatggacg aaggcatgtc
     6061 gcacctgatc ctgctggcgg tgccgctgtt catcttcctg ggcctgctga tcgagatgac
     6121 cggcatggcg cagcgcatgg tggccttcct ggccaacctg atcggccaca agcgcggcgg
     6181 cctggcctac gtgctgatcg gcgccatgta cctcgtctcg ggcatctccg gcgccaaggc
     6241 cgccgacatg gcggcggtgg cgccggcgct gtttccggag atgaagcggc gcggcgccaa
     6301 ggagggcgac ctggtggcgc tcctggcggc caccgccgcg cagaccgaga ccatcccgcc
     6361 cagcatcgtg ctgatcacca tcggctcggt caccggggtg tccatcgccg ccctgttcac
     6421 cggcggcctg ctgccgggcg cgttgctcgc ggcggccctg tgctgcgtgg tgtggcgccg
     6481 ctatcgccgc gaggacatgt ccggcgccgc ctggccgggc cgcggcgcga tctggcgctc
     6541 gctgcggatc gccctgccgg cgctggccct gcccttcgtg atccgcttcg ccgtggtcga
     6601 cggcgtggcc accgccaccg aggtgtcgac catcggcatc gtctacgccc tggtggtcgg
     6661 cctgctggtg taccgctgtt tcgaccggtc gcggctggtg cccatgctgg tcgagacggc
     6721 ggcgctgtcc ggcgccatcc tgctcatcat cggcacggcc acggccatgg cctggtgcct
     6781 gacccgctcc gggttctcca acgacctggc cgcgctgatg gccgggctgc cgggcggcaa
     6841 actgggcttc ctgggcgtgt ccatcctgat gttcatcgtg ctgggcagcg tgctggaagg
     6901 cgtgccggcc gtggtgctgt tcgggccgct gctgtttccc atcgcccagc agatgggcgt
     6961 gcacgaagtg cactatgcca tggtggtgat cctgtcgatg ggcatcggcc tgttcgcgcc
     7021 gcccttcggc gtgggctatt acgtcgccgc ggccataggc aaggcctctc ccgatgccgg
     7081 catgcgcccc gtcgccggct atctgctggc gctgttcatc ggcgtgctgg tcatcgccgc
     7141 ggtgccgtgg atttccacgg ggttcctccc atgaacggcg cttccgaggg gcaacccgcc
     7201 atgaagatat tcctggtcgg cgaggccgcc acccatgccg acgaactggt gcagcacctg
     7261 caaaccccgg cgcagctggt gccgctgccg cgcgaggcgg cgcacgacgc cggccatgac
     7321 gcgctgatcg gcccgcagga cgtggtggtg tcgctgcgct tcgcgcgccg cggcgaggcc
     7381 ccgccctttc gcctgctgca cgtgccgggc gccggcctgg acggcatcga cacggcctgc
     7441 ctgccgccgg gctgcgccgt gtgcaatgtg ttcgaacacg agattcccat cgcggaatat
     7501 gtctgcctgg cgatgctgga atggcaggtc cggctgcgcg ccctgacggc ctcgttcacg
     7561 gccgaggcct ggcccgacct gtaccgcgac cgccggcccc atggcgagct gcatggcaag
     7621 accctggcgc tgatcggcct gggccggatc ggccgcgcca ttgcgcagcg cgccggcgcc
     7681 ttcggcatgc gggtgctggc ggtggacggc catgcgccgc agccgccgca ggggctggtc
     7741 gagcgcctgg tcgcgccgca cgactgggaa gaggccctgg agcaggccga cttcgtcgcc
     7801 atcacctgcc cgctcacccc gcagacgcgc ggcctgttca atgcccgcag cctgcggcgc
     7861 atgcggcgcg acgcggtgct gatcaacgtc tcccgcgccg agatcgccga cgagcgcgcg
     7921 ctctacgagg cgctgcgcga ccgggtcatc ggcggcgcgg tgctggacgt ctggtaccgc
     7981 tacccgcagg gcgccggcga ccgggtcgcg ccgtcggacc agccgttcct ggacctgccc
     8041 aacgtgatcg ccacgccgca ctcctcggcc tggaccgacg gcctgccccg gcgccgctac
     8101 gccttcatcg cccgcaacat cgaccggctg gttcgcggcg agccgctgca gaaccgggtg
     8161 cgggccccct gacccgcgcc gcttcgccta gctagtattt gcgtgctgga aatattttat
     8221 gtgctgattc tcatatattg aaattatgtg ccaaatcaca taatttcaat atatgtgcca
     8281 attacgatat tgacgaatta tcgtgattgg cgcatagttc aggccaggag accattatgt
     8341 ccaaccttgc ccgcacgccc cagcagatcg gcgctcttat ccgccggaac cggaagcagc
     8401 tcggcttgag ccaaagtgcc ttgggcgagc gcgtaggact ccggcaggaa acgatctcgc
     8461 tgatcgagac cggcaatccg gccacccggc tggacacttt gctagccgtg ctggccgcgc
     8521 tggatctgga acttcagatc gccgcccgca cccgccagca cgatgtcgcg gatctgatcg
     8581 aataatggcc agaacccgcc ggcaccctcc ccttcgggtt tatcaaaaca accgattggt
     8641 gggacacctg ctcaagtcgc cgaacggggc gatagagttc cgttatgacg ccacgtggct
     8701 ggaccgaagc acggctttcc cggtatcgct ttcgctgccg ctgcaggagg cgccatggcg
     8761 gggcgagcct gtcgtagcgg tgttcgagaa cctgctgccg gattccgagg tcttgcgaag
     8821 gcgagtggcc gagcgcgtcg gcgccgcagg gtccgacgcc tacagcttgc tggccgcgat
     8881 tggcagggac tgcgtgggcg ccttgcaatt cgttgctggc gatgctgccg aaccagacac
     8941 gaacgcgggt atctcgggcg aacctgtcga tggcgaagcg atcgagcgtc ttctcaagtc
     9001 gttggggcag gctccgctgg ggctgtcccg ggacgacgat ttcagaattt cagtcgccgg
     9061 cgcccaagag aaaaccgcac tgcttcaagt cgacgggcaa tggttcaagc cccacggcac
     9121 gacgccgacc acgcacttgt tcaagacgcc gatcggcaag ctgcccaacg gtattgacct
     9181 atccaacagc gtcgagaacg agttctactg cctgaggttg ctcagccacc tgggcttgcc
     9241 ggtcaatcac gccgagatca tgaccttcgg ccagaccaag gccttggtca tcgagcggtt
     9301 cgaccgggcc tggcgcaatg gccgcctgat ccgtcttccg caggaagatt gctgccaggc
     9361 gctatccgtg ccgccggcac gcaagtacca gagcgatggc ggccccggca tgctcgacat
     9421 cgcgaatctg ctcaagggca gtgactcgcc agccgacgat catcgcac
//
LOCUS       DASWPO010000084        11752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_198030, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000084 DASWPO010000000
VERSION     DASWPO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198030"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VGC69_04930"
     CDS             complement(<1..745)
                     /locus_tag="VGC69_04930"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0294664.1"
                     /translation="MFRNILTDITVVDFTALGAGPMCTMLLGDMGANVIKVEPPQGEL
                     GRTLGPGWIGEDCTAYYGFNRNKRSISIDLKTERGRQLAQRLIQTADVVVESMRPGTM
                     KKLGLDYDSVRGAHEKLIYCSISAYGQAGPYSGRPGVDGILQADCGLMSIIGSRDSAE
                     PCKVQAPVVDVTTGYIACLAVVAQLLDLQRQGKGGYIDVNLMNSALTLQQTSITNYLS
                     EQVVPEKMGSAAPYSAPNEAFPTQDGWIMV"
     gene            complement(765..1757)
                     /locus_tag="VGC69_04935"
     CDS             complement(765..1757)
                     /locus_tag="VGC69_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294665.1"
                     /translation="MQVGNTLRLGGAALALIASSLLPAAPAAAQDDAPLTFVVPFTPG
                     GGNDVLARLLAPHLSQALHRNVIVENKAGASGNIGLGYVAHSKPDGNTIGIAGSQVAT
                     NPAMGMPVSFDVQKDLAPVGMIAEVPLVLVVNAKLPYKTLDEFVAYARQHPGEINFGS
                     PGVGVPQHLAGELLDDMAHIKMTHVPYQGLSPALSGVLSGQIQAVFGDLGAVLPYVHD
                     GSMRALGVARNRRTALVPDMPAFNEDHGVGLDNYNAAMWFSIMAPARTPKPIIDKLNA
                     ALNESLKNPDVQKQLADQGFEISRTTPEQLSEIVSRDLALWTRVADENHLIAKN"
     gene            complement(1797..2444)
                     /locus_tag="VGC69_04940"
     CDS             complement(1797..2444)
                     /locus_tag="VGC69_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0294666.1"
                     /translation="MDNQKLILTKQDQVVEQIREQIIAGEYERGERLKQADIALKLGV
                     SVTPVREALKALEMEGYVRSFPNRGIYIPEYSGKDIQEIFRLRLMLEEFLVTQALESI
                     RQPDIDDLRRIQDQFLACLHEQDYVGMRIANVKFHFKLYEAAGCQQTLQFVRVLWAKY
                     PFGFQDQKNIRRFELINREHQAILDMLQAGDSQGVLDHFRKHLTAGKQWLDKLSP"
     gene            complement(2454..2885)
                     /locus_tag="VGC69_04945"
     CDS             complement(2454..2885)
                     /locus_tag="VGC69_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY0294667.1"
                     /translation="MNDIAKDIAKTPAPVLGMYDAPMWESIRAGGMTLQCCRECRVFQ
                     YPPAPVCHACLSSELDWTPVGGEGEILSWVVYHRQYLPAYPAPYNVISVRLKEGPVLV
                     SNLEGSVPEGSWIGRKVRLRYAEMPDGFVLPRFALDAPGAP"
     gene            complement(2888..4090)
                     /locus_tag="VGC69_04950"
     CDS             complement(2888..4090)
                     /locus_tag="VGC69_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0294668.1"
                     /translation="MSKSPSSFQAQGTPSSRLSRAAAVVGVGHTDWIRDWHAVRAGEK
                     PTDSYGYAAGAFKRALDDAGLSREDIDGLIVGPTTAYERMSEVLGINPRWGDQADAVL
                     SVLQAVSAIESGLAETVALVYGNDQRSGQVQYGGPQAMGGDAFLSYVYHAPWGLTSQG
                     ALYALTFQAYRHAWGFQEADLARVSVAQRQWASLNPNAVMQKTITVEDYLAAPYICEP
                     LRLYDYCMINDGGVALILTTADRAKRFKNKPPVYVQGLGRRDLNGYATSLEPRLTEFY
                     YPAQQDCARQAFEMAGVGPGDIDVLQVYDSFSVHVPLALEGYGYCARGEAGKFLRESG
                     MGPGGKLPINTSGGHLSESYMQGWAHQIESVRQLRGECGARQVDGCDWVHYTSDVAGK
                     AVSIIYGK"
     gene            4293..5687
                     /locus_tag="VGC69_04955"
     CDS             4293..5687
                     /locus_tag="VGC69_04955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0294669.1"
                     /translation="MATNTAAKKQILLNAFNMNCVGHINHGLWTHPRDTSSQYKTLDY
                     WTRLARLLERGLFDGLFLADIVGVYDVYQDSVDLTLKEAIQLPVNDPLLLVSAMAAAT
                     RHLGFGVTVNLTYEAPYLLARRFSTLDHLSNGRIGWNIVTGYLDSAARAMGLDEQIGH
                     DERYDRADEFMDVAYQLWEGSWDDDAVRNDKSARVYADPDRVRRIDHQGRYYRVQGHH
                     LAEPSPQRTPLLYQAGSSGRGQAFATRHAECVFVSAQSPEALRAQVRGAREAVARAGR
                     DPQSIKFFMGVTVVVGRNEHEAREKFADYARYASPEAGLAHYASSTGVDFSRYGLDDP
                     IRHGKTNAMESAARTLSTPAGGERTVRDLLGGLTLGGRYPAIVGSPQQVADALEAWVR
                     DTDIDGFNLTRTVTPECYEDFIDLVVPALQARGSYKTAYSPGTLREKLFGAGRARLPA
                     GHAGAAVSAPSAAR"
     gene            complement(5707..6555)
                     /locus_tag="VGC69_04960"
     CDS             complement(5707..6555)
                     /locus_tag="VGC69_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0294670.1"
                     /translation="MQTFTLKSLTLIGALVGAMLAASPAFSADPNKKEIVFGATAGPY
                     SDQIKLGIKPILEKEGYTVKIVEFNDYVQPNFALAQGSLDANAFQHEVYLKTFSSKNN
                     LALSELVKVPTAPIAVYSKKYHSVAVAEVPDGVSVALPNDPTNEARALVMLDKQLHWL
                     TLREGYDPITVSEKDVAGNPKKIKLVPIEAAQLPRTLDDVDFSFVNGNFALASGLTLP
                     QALALEPTTPTYQNLVAVRTADLDKPFARDIAAAYQSREFLAVTEKSFPGFVKTGYQQ
                     KLEAAQ"
     gene            complement(6584..7246)
                     /locus_tag="VGC69_04965"
     CDS             complement(6584..7246)
                     /locus_tag="VGC69_04965"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEY0294671.1"
                     /translation="MLDTFIQLLPELAVALGQTFLMLGIALGASIVLGGPLGILLFLT
                     GSGQSLDRPVLHRALSWIVNVVRSFPFIILLVALVPFTRVIVGSSIGPTAAAVPLSFA
                     AIPYFARLVEQCLREVPRGVVEAAHAVGASELQIVRRVLLVEARSGLVLALTVLAVSF
                     LSYSAVAGVVGGGGIGDLAIRYGYYRFQTDVMVITVAVLVVLVQIIQFAGNHIAQRID
                     KR"
     gene            complement(7246..8067)
                     /locus_tag="VGC69_04970"
     CDS             complement(7246..8067)
                     /locus_tag="VGC69_04970"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEY0294672.1"
                     /translation="MIPPKTLAVVGAVEPAAARPVIRLDGVSKHFSTREGRFDAVRNV
                     SLSIQPGDTFGLIGKSGAGKSTLLRLVNLLERPDAGAVHVAGIELTGLGKRALREARQ
                     SIGMIFQQFNLLQNEPVFENVAFALRVHGGRSQAQITARVDECLDIVGLADKRGSYPA
                     QLSGGQKQRVAIARALASEPAVLLCDEPTSALDPETTRSVLGVLRDINTRLGVTIVIV
                     THELAVVRALCRNVAVMENGQIVEQAEITRAGVSLATALGRELIREASHPYEEVA"
     gene            complement(8090..9265)
                     /locus_tag="VGC69_04975"
     CDS             complement(8090..9265)
                     /locus_tag="VGC69_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0294673.1"
                     /translation="MPTSVGPTIDVIVDDLAKKLAANAVERDRVGGHAAAERELIRAS
                     GLLHLSIPRKYNGLGADWSTILGIVRRLARVDSALAHVFGFHHLQIAGVLLYGGPAQH
                     EHFLPPTVQGNLFWGNALNPLDRRVIAADEPGGFRLDGVKSFSSGSVGSDVLTISAWH
                     RASDTALVAALPTHTEGITVNADWDAFGQRQTDSGTVRFDHVRLASLQVLQAPGISAT
                     PKATLRTQLAQLVMTNLYLGIAQGALAEARRFVQEDARPWFAADVQRQADDPYVQARF
                     GEFRLVARAAEALADAAGKQLDAALGRGDEVTAAERGELAVASAEAKVWSHRAALQVT
                     SELFDATGARSASGRYGYDRFWRNARVHTLHDPVAYKIRDLGRYELDGLVPEPTAYS"
     gene            complement(9340..10512)
                     /gene="ssuD"
                     /locus_tag="VGC69_04980"
     CDS             complement(9340..10512)
                     /gene="ssuD"
                     /locus_tag="VGC69_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HEY0294674.1"
                     /translation="MNIFWFIPTHGDSRYLGTSTGARAASYDYFRQVAVAADTLGYDG
                     VLLPTGRSCEDAWVVASSLIGATQRLKFLVAVRPGVTSPQFAARMAASFDRVSSGRLL
                     VNVVTGGDTGELEGDGLFISHDERYEITRDYLKIWRGILEASHAGGDFSFEGEQLTAK
                     GGKVIYPPVQKPYPPLYFGGSSEAAHQLAAEQLDVYLTWGEPPDAVRAKIADIRARAA
                     KVGRTLAFGIRLHVIVRETNEAAWAAADELISHLSEETVAAAQKGLSRLDSEGQRRMS
                     ELHGGKLHDGQGGHSHLEIAPNLWAGVGLVRGGAGTALVGDPQTVAERIREYADLGID
                     TFIFSGYPHLEESYRFAELVFPLLPGKLQKQLGNNVLTGPFGEVMATELVPRASAS"
     gene            complement(10542..11342)
                     /locus_tag="VGC69_04985"
     CDS             complement(10542..11342)
                     /locus_tag="VGC69_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0294675.1"
                     /translation="MSKLLRALSVPVLALAVSFTASAADKSSIKVGISVGSAEQIFNV
                     VKRVAKRDGLDIDVVVFNDYQLPNAALAAGDLDANAFQHKPFLDAQIKARGFDLAPVG
                     LTVTAPLGFYSHKIKALQDLPEGASVGIQNDPSNGNRALLVLQAYKLITLKPEAVQNN
                     TATPLDIVANPRKLKIVQLDAAQLPRSLDDLTAASINNDFAEKAGLVPGRDAIGREAP
                     DGPYANLIAVRRADKDQPWVKKLVAAYQSPEVRDFINAEYKGSLIPAF"
     gene            complement(11452..>11752)
                     /locus_tag="VGC69_04990"
     CDS             complement(11452..>11752)
                     /locus_tag="VGC69_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0294676.1"
                     /translation="DRSADAIQADAPNASDLVLESALVTAKTQLVVSQLALDAAEKLF
                     NAGGASMTGRKHNFDRHWRSLRTLFSHNPLLHKARVVGDYHLNGTTTHLLEGKVF"
BASE COUNT         1899 a         3958 c         3927 g         1968 t
ORIGIN      
        1 ccaccatgat ccagccatcc tgcgtcggaa aggcctcgtt gggcgcggaa tacggcgcgg
       61 cgctgcccat cttctccggc acgacctgct cgctcaggta gttggtgatc gaggtctgct
      121 gcagcgtcaa cgccgagttc atcaggttga cgtcgatgta gccccccttg ccctggcgct
      181 gcaggtccag caactgggcc accaccgcca ggcaggcgat gtagccggtg gtcacgtcga
      241 cgaccggcgc ctgcaccttg cacggctcgg cgctgtcgcg gctgccgatg atgctcatca
      301 gtccgcagtc ggcctgcaga atgccgtcca ccccgggccg gcccgaataa gggccggcct
      361 gcccataggc cgagatcgaa caatagatga gcttctcgtg ggcgccccgc acgctgtcgt
      421 agtccaggcc cagcttcttc atcgtgccgg ggcgcatgct ctcgacgacc acgtcggcgg
      481 tctgaatcag ccgctgcgcc agctgccggc cccgctccgt cttcaggtcg atgctgatcg
      541 agcgcttgtt gcggttgaac ccgtagtagg cggtgcagtc ctcgccgatc cagcccgggc
      601 ccagggtgcg ccccagctcg ccctgcggtg gttcgacctt gatgacgttg gcgcccatgt
      661 cgcccagcag catggtgcac atcggccccg cgcccagcgc ggtgaagtcg acgacggtga
      721 tatcggtgag tatgttcctg aacatgcggg ctcctgttgc tgcgtcagtt cttggcgatc
      781 agatggttct cgtccgccac ccgggtccag agcgccaggt cgcgcgacac gatctcgctc
      841 agttgctcgg gggtcgtgcg ggaaatctcg aagccctggt cggccagctg cttctgcacg
      901 tcggggttct tgagcgattc gttcagcgcg gcgttgagct tgtcgatgat gggcttgggc
      961 gtcctggccg gcgccatgat cgagaaccac atggcggcgt tgtagttgtc cagcccgacg
     1021 ccatggtcct cgttgaaggc cggcatgtcc ggcaccagcg ccgtgcgccg gttgcgggcc
     1081 acgcccagcg cgcgcatgct gccgtcgtgg acgtacggca gcaccgcgcc caggtcgccg
     1141 aagacggcct ggatctggcc gctcagcacg ccggacagcg ccggcgaaag gccctggtac
     1201 ggcacgtgcg tcatcttgat gtgggccatg tcgtcgagca gctcgccggc caggtgctgc
     1261 ggcacgccca cccccgggct gccgaagttg atctcgcccg ggtgctgcct ggcgtaggcc
     1321 acgaactcgt ccagcgtctt gtagggcagc ttcgcgttga ccaccaggac cagcggcacc
     1381 tcggcgatca tgcccaccgg cgccaggtct ttctgcacgt cgaacgacac cggcatgccc
     1441 atggccggat tggtggccac ctggctgccg gcgatgccga tggtgttgcc gtcgggcttg
     1501 gaatgcgcca cgtagcccag gccgatgttg cccgacgcac cggccttgtt ctccacgatc
     1561 acattgcggt gcaatgcctg cgacaggtgg ggcgccagca ggcgcgccag gacgtcgttg
     1621 ccgccgcccg gcgtgaacgg cacgacgaag gtcaagggcg cgtcgtcctg cgcggcggcc
     1681 ggggccgccg gcagcaggga cgatgcgatg agggcgagcg ccgccccgcc gagtctcagc
     1741 gtatttccga cttgcatggt tttgtctccg agtgttttct tgtaaaagtc gccccgtcag
     1801 gggctaagct tatcgagcca ttgtttgcct gccgtcaggt gtttgcggaa gtgatccagc
     1861 acgccctggc tgtccccggc ctgcagcatg tccaggatgg cctggtgctc gcggttgatc
     1921 agctcgaacc gccggatatt cttctggtcc tgaaaaccga acgggtactt ggcccacaat
     1981 acccgcacga actgcagcgt ctgctggcag ccggctgctt cgtacaactt gaagtggaac
     2041 ttcacgttgg cgatgcgcat gcctacatag tcctgctcat gcaggcaggc cagaaactga
     2101 tcctggatgc gccgcaggtc atcgatgtcg ggctgccgga tgctctccag cgcctgggtc
     2161 accaggaact cctccagcat cagccgcagc cggaagatct cctggatgtc cttgcccgag
     2221 tactcgggga tgtagatgcc gcgattcgga aagctgcgca cgtagccttc catctccaac
     2281 gccttcagcg cttcgcgcac cggcgtcacc gagacgccga gcttgagcgc gatgtccgcc
     2341 tgcttcaggc gttcgccgcg ctcgtactcg cccgcgatga tctgctcgcg gatctgctcg
     2401 accacctggt cttgcttggt gaggatgagt ttctggttgt ccatgtgtct ttgtcagggc
     2461 gcgccgggcg catcgagggc gaaacggggc aggacgaagc cgtccggcat ttccgcgtag
     2521 cgtagcctga ctttgcggcc tatccacgaa ccctcaggca cgctgccctc caggttggac
     2581 accagcaccg gcccttcctt gagccggacg gagatcacgt tgtagggcgc gggataggcc
     2641 ggcagatact ggcggtggta gaccacccac gacaggatct cgccctcccc gccgaccggc
     2701 gtccagtcca gctcggacga caggcaggca tggcagaccg gcgcgggcgg gtactggaaa
     2761 acccggcatt cgcggcagca ctgcagcgtc atgccgccgg cgcggatgct ctcccacatg
     2821 ggcgcgtcgt acatgcccag cacgggcgcg ggggtcttgg cgatatcctt ggcgatgtcg
     2881 ttcatgctca tttcccgtag atgatcgaca cggccttgcc cgccacgtcg gaggtgtagt
     2941 gtacccagtc gcaaccgtcg acctgccgcg cgccgcactc gccgcgcagc tgccgcaccg
     3001 actcgatctg gtgcgcccag ccctgcatat agctttccga cagatggccg ccgctggtgt
     3061 tgatgggcag cttgccgccc ggccccatgc cggactcgcg caggaacttg cccgcctcgc
     3121 cgcgcgcgca gtagccgtag ccttccagcg ccagcggcac gtgcaccgaa aagctgtcgt
     3181 acacctgcag cacgtcgatg tcgcccgggc ccacgccggc catctcgaaa gcctggcgcg
     3241 cgcagtcctg ctgggcgggg tagtagaact cggtcaggcg cggctccagg ctggtggcgt
     3301 agccgttcag gtcgcgccgg cccagccctt gcacgtacac cggcggcttg ttcttgaagc
     3361 gcttggcgcg gtcggccgtg gtgaggatca gcgccacccc gccgtcgttg atcatgcaat
     3421 agtcgtacag ccgcagcggc tcgcagatat agggcgccgc caggtagtcc tcgaccgtga
     3481 tggtcttttg catgaccgcg ttggggttga gcgaggccca ctggcgctgg gccaccgaca
     3541 cccgcgccag gtccgcttcc tggaagcccc aggcgtggcg ataggcctgg aaggtcagcg
     3601 catacagcgc gccctgcgaa gtcagccccc agggcgcgtg gtacacgtag gagagaaaag
     3661 cgtcgccgcc catggcctgg gggccgccgt actgcacctg ccccgagcgc tggtcgttgc
     3721 cgtacaccag cgccacggtc tcggccagcc cgctctcgat ggcgctgacc gcctgcagca
     3781 ccgacagcac cgcgtcggcc tggtcgcccc agcgcgggtt gatgcccagc acctccgaca
     3841 tgcgctcgta cgccgtggtc ggccccacga tcaggccgtc gatgtcctcg cgcgacaggc
     3901 cggcatcgtc cagcgcgcgc ttgaacgcgc cggcggcata gccgtagctg tcggtgggct
     3961 tctcgccggc gcggacggcg tgccagtcgc ggatccagtc ggtgtgcccc accccgacca
     4021 ccgcggcggc ccggctcagg cggctcgagg gcgtgccctg ggcctgaaaa ctgctggggg
     4081 atttgctcat gcgcgcttgt ctccttccgg gttgatttat tttttgtaga ttatcaaaaa
     4141 taaattattt ttcagatact aaatgtaaat aaaaaggaat aaacccgatg gccgccgcac
     4201 ataaccgtcg cgcataaccg catgaacaaa ctgtccttcg ccgcgccgtg gcgcggccct
     4261 aggatgagca gttttccagg cggtttgttt ccatggcgac gaatacagcg gcaaaaaagc
     4321 agatcctcct gaacgcgttc aacatgaact gcgtgggcca catcaaccac ggcctctgga
     4381 cccatccgcg cgacacctcg tcgcaataca agaccctgga ctactggacc cgactggccc
     4441 gcctgctcga gcgcggcctg ttcgacgggc tgttcctggc cgatatcgtc ggcgtctacg
     4501 acgtctacca ggacagcgtg gacctcaccc tgaaagaagc catccagttg cccgtcaacg
     4561 acccgctgct gctggtttcc gccatggcgg cggccacgcg gcacctgggt ttcggcgtca
     4621 ccgtcaacct gacctacgaa gcgccctacc tgctggcccg gcgcttctcg acgctggacc
     4681 acctgagcaa cggccgcatc ggctggaaca tcgtcaccgg ctacctggac agcgcggcgc
     4741 gcgccatggg cctggacgag cagatcggcc acgacgagcg ctacgaccgc gccgatgagt
     4801 tcatggacgt ggcctaccag ctgtgggagg gcagttggga cgacgacgcc gtgcgcaacg
     4861 acaagtccgc gcgcgtctat gcggacccgg accgggtgcg ccgcatcgac caccaaggcc
     4921 gctactaccg ggtgcagggc catcacctgg ccgagccctc gccccagcgc acgcccctgc
     4981 tctaccaggc gggatcttcc ggccgggggc aagccttcgc cacccggcat gcggagtgcg
     5041 tcttcgtctc cgcgcaatcg cccgaggcgc tgcgcgcgca ggtgcgcggc gcccgcgagg
     5101 ccgtcgcgcg cgccggacgc gatccgcaga gcatcaagtt cttcatgggc gtcacggtgg
     5161 tggtcggccg caacgaacac gaagcccgcg agaaattcgc cgactacgcg cgttatgcca
     5221 gtcccgaggc cggcctggcg cactatgcca gctcgaccgg cgtggatttc tcccgctatg
     5281 gattggacga ccccatccgg cacggcaaga ccaacgccat ggaatcggcc gccaggacgc
     5341 tgtccacgcc cgccggcggc gaacgcaccg tgcgcgacct gctgggcggg ctgacgctgg
     5401 gcggccgcta tccggccatc gtgggctcgc cgcagcaggt ggccgacgcg ctggaggcct
     5461 gggtgcgcga caccgacatc gacggcttca acctgacgcg caccgtcacg ccggaatgct
     5521 atgaagactt catcgacctg gtggtgccgg cattgcaggc gcgcggttcg tacaagacgg
     5581 cctactcgcc cggcacgctg cgcgagaagc tgttcggcgc gggccgcgcc aggctacccg
     5641 cgggccatgc cggggccgcc gtatcggccc catccgctgc ccgataggcc ccggcccgcg
     5701 cgttgattac tgggcggctt ccagcttctg ctggtagccg gtcttgacga aaccggggaa
     5761 agatttttcc gtcaccgcca ggaactcgcg cgactggtag gccgccgcga tgtcccgggc
     5821 gaagggtttg tccaggtcgg ccgtgcgcac cgccaccagg ttctggtagg tgggcgtggt
     5881 gggctcgagc gccagcgcct gcggcagcgt caggccggaa gccagggcga agttgccgtt
     5941 gacgaacgag aaatcgacat cgtccagcgt gcgcggcaac tgcgccgcct cgatcggcac
     6001 cagcttgatc ttcttggggt tcccggcgac gtccttctcg gacaccgtga tggggtcgta
     6061 gccttcgcgc agcgtgagcc agtggagctg cttgtcgagc atgaccagcg cgcgcgcctc
     6121 gttggtgggg tcgttgggca gggccaccga gacgccgtcc ggcacttcgg ccacggccac
     6181 gctgtggtac ttcttgctat agacggcgat gggcgcggtc ggcaccttga ccagctcgga
     6241 cagggccagg ttgttcttgc tgctgaaggt cttgaggtag acctcgtgct ggaacgcgtt
     6301 ggcgtcgagc gaaccctgcg ccagggcgaa attgggctgc acgtaatcgt tgaattcgac
     6361 gatcttcacc gtatagccct ctttctccag gatgggcttg atgcccagct tgatctggtc
     6421 gctgtagggg ccggccgtgg cgccgaagac gatctctttt ttgttcggat cggcgctgaa
     6481 cgcggggctg gccgccagca tggcgccgac cagggcgccg atgagcgtga gggacttcag
     6541 ggtaaacgtc tgcatggtct cgagggtctc cggaaaagga agttcaacgt ttgtcgatgc
     6601 gctgcgcgat atggttgccg gcgaactgga tgatctgcac cagcaccacc agcacggcca
     6661 ccgtgatcac catcacgtcg gtctggaagc ggtaatagcc gtagcggatg gccaggtcgc
     6721 cgatgccgcc gccgcccacc acgccggcca ccgccgaata ggaaagaaag ctcacggcca
     6781 gcactgtcag ggccagcacc aggccggagc gcgcctcgac cagcagcacg cggcgcacga
     6841 tctgcaattc ggacgcgccc acggcatgcg cggcctcgac cacgccgcgc ggcacttcgc
     6901 gcaggcattg ctcgaccagc cgggcaaagt acgggatggc cgcgaacgac agcggcaccg
     6961 ccgccgccgt ggggccgatg gacgagccca cgatcacccg cgtgaacggc accagcgcca
     7021 ccagcaggat gatgaaggga aacgagcgca cgacattgac tatccagctc agcgcgcgat
     7081 gcagcacggg ccggtccagg gactgccccg acccggtcag gaacagcagg atgccgagcg
     7141 gtccgcccag cacgatcgag gcgcccagcg cgatgcccag catcaggaag gtctgcccca
     7201 gggccacggc cagctcaggc agcaactgga tgaaagtgtc cagcatcacg ccacctcttc
     7261 gtagggatgc gaggcctcgc ggatgagctc gcggcccagc gccgtggcca ggctgacgcc
     7321 ggcgcgggta atttcggcct gctcgacgat ctggccgttt tccatcacgg ccacgttgcg
     7381 gcacagcgcg cgcaccacgg ccagctcatg cgtgacgatg acgatggtca cgcccagccg
     7441 ggtgttgatg tcgcgcagca cgcccagcac cgagcgcgtg gtctcggggt cgagtgccga
     7501 ggtcggctcg tcgcacagca gcaccgccgg ctcgctggcg agcgcccggg cgatcgccac
     7561 gcgctgcttc tgcccgcccg agagctgggc gggatagctg ccgcgcttgt cggccaggcc
     7621 cacgatgtcc aggcactcgt cgacccgcgc ggtgatctgc gcctggctgc gcccgccgtg
     7681 cacgcgcagc gcaaaggcca cgttctcgaa caccggctcg ttctgcagca ggttgaactg
     7741 ctggaagatc atgccgatgg actgccgcgc ctcgcgcaag gcgcgcttgc ccaggcccgt
     7801 cagttcgatg ccggccacgt gcacggcccc cgcatcgggc cgctccagca agttgaccag
     7861 ccgcagcagc gtggacttgc ccgcgccgct cttgccgatg aggccgaagg tgtcgcccgg
     7921 ctggatggac agcgacacat tgcgcaccgc atcgaagcgg ccctcgcggg tcgagaaatg
     7981 cttgctcacg ccgtcgagcc ggatgacggg ccgcgccgcg gccggctcca ccgcgccgac
     8041 cacggcaagc gtcttggggg ggatcacgga aggactcaat gcagccgcct cacgagtacg
     8101 ccgtgggctc gggcacgagc ccgtcgagct cgtagcggcc caggtcgcgg atcttgtagg
     8161 ccaccggatc gtgcagcgtg tgcacccggg cattgcgcca gaagcggtcg tagccgtagc
     8221 gccccgaagc cgagcgcgcg cccgtcgcgt cgaacagctc gctggtcacc tgcagggcag
     8281 cgcggtgcga ccagaccttg gcctccgcgc tggccaccgc cagttcgccg cgctcggccg
     8341 ccgtgacctc atcgccgcgg cccagcgcgg cgtccagctg cttgcccgcc gcgtcggcca
     8401 gcgcctcggc ggcgcgcgcc accagccgga attcgccgaa acgcgcctgc acataaggat
     8461 cgtcggcctg gcgctgcacg tcggcggcga accagggccg ggcgtcctcc tgcacgaagc
     8521 ggcgcgcctc ggccagggcc ccctgggcaa tgcccaggta cagattggtc atgaccagct
     8581 gggccagctg ggtgcgcagc gtggccttgg gggtggccga gatgccgggc gcctgcagca
     8641 cctgcagcga cgccagccgc acgtggtcga agcgcacggt gccgctgtcg gtctggcgct
     8701 ggccgaaggc atcccagtcg gcgttgacgg taatgccctc ggtatgcgtg ggcagggccg
     8761 cgaccaatgc ggtgtccgag gcgcggtgcc aggccgagat cgtcagcacg tccgaaccca
     8821 ccgagcccga gctgaagctt ttcacgccgt cgagccggaa cccgcccggc tcgtcggccg
     8881 cgatcacgcg gcggtccagg gggttgagcg cattgcccca gaacagattg ccctgcaccg
     8941 tgggcggcag gaaatgctcg tgctgcgcgg gcccgccata cagcagcacg cccgcgatct
     9001 gcaaatggtg gaacccgaac acgtgcgcca gggcgctgtc gacccgggcc aggcggcgca
     9061 cgatacccag gatggtcgac caatcggccc ccaggccgtt gtacttgcgg ggaatggaca
     9121 ggtgcagcag gccgctggcg cggatcagct cgcgctcggc cgccgcgtgg ccgccaacgc
     9181 gatcgcgctc gaccgcgttg gcggccagtt ttttcgccag gtcgtcgacg atgacgtcga
     9241 tggtcggccc gaccgacgtc ggcatgccga acgccgaggt gatcagcggg ttgtcgaaaa
     9301 catgctggaa tagggaaatc tttgccatgg tggttccggt caactggccg acgcgcgcgg
     9361 cacgagctcg gtcgccatga cctcgccgaa cggcccggtg agcacgttgt tgcccagttg
     9421 cttttgaagt ttgcccggca gcagcgggaa gaccagctcg gcgaagcgat aggactcttc
     9481 caggtgcggg tagccggaga agatgaaggt gtcgatgccc aggtcggcgt attcgcggat
     9541 gcgctcggcc acggtttgcg gatcgcccac cagcgccgtg ccggcgccgc cgcgcaccag
     9601 gcccaccccc gcccacagat tgggcgcaat ctcgagatgc gaatggccgc cctggccgtc
     9661 gtgcagcttg ccgccgtgca attcggacat gcgccgctgg ccttccgaat ccaggcgcga
     9721 cagccccttc tgcgcggcgg ccacggtttc ctcgctgaga tggctgatca gttcgtcggc
     9781 cgccgcccag gcggcttcat tggtttcgcg cacgatgacg tgcaggcgga tgccgaatgc
     9841 cagcgtgcgg cccactttgg ccgcgcgcgc gcgaatgtcg gcgatcttgg cgcgcaccgc
     9901 gtcgggcggc tcgccccagg tcaggtagac gtcgagctgc tcggccgcca actggtgggc
     9961 ggcttccgac gagccgccga aatacagcgg cggatacggt ttctgcaccg gcgggtagat
    10021 caccttgccg cccttggcgg tgagttgctc gccctcgaaa ctgaaatcgc cgcccgcgtg
    10081 gctggcctcg aggatgccgc gccagatctt caggtaatcg cgggtgatct cgtagcgctc
    10141 gtcatggctg atgaacaggc catcgccttc cagctcgcct gtgtcgccgc ccgtgaccac
    10201 attgacgagc agccggcccg aggacacgcg gtcgaacgag gcggccatgc gcgcggcgaa
    10261 ctgcggcgag gtcacgccgg gccgcaccgc caccaggaac ttgagccgct gcgtcgcccc
    10321 gatcaggctg gaggccacca cccaggcgtc ctcgcaggag cgcccggtgg gcagcagcac
    10381 gccgtcatag cccagcgtgt cggccgccac ggcaacctgg cggaaatagt cgtagctggc
    10441 cgcgcgggct cccgtgctgg tgcccaggta gcgcgaatcg ccgtgggtgg gaataaacca
    10501 gaagatattc atgacgttct cgttgtttct ctggcccgcg gtcagaaagc ggggatcagc
    10561 gaacccttgt attcggcatt gatgaagtcg cgcacctcgg gcgactggta ggccgccacc
    10621 agctttttga cccagggctg gtccttgtcg gcacggcgca ccgcgatcag gttggcatag
    10681 gggccgtcgg gcgcttcgcg gccgatcgca tcgcgtccag gcaccaggcc ggccttctcg
    10741 gcgaagtcgt tgttgatcga cgcggccgtc aggtcgtcga gcgaacgcgg caactgcgcg
    10801 gcgtccagct gcacaatctt gagcttgcgg gggttggcga cgatgtccag cggcgtggcg
    10861 gtgttgttct gcacggcctc gggcttgagg gtgatgagct tgtaggcctg cagcaccagc
    10921 aaggcgcggt tgccgttgga ggggtcgttc tggatgccca ccgaagcgcc ttcgggcaag
    10981 tcctgcagcg ccttgatctt gtgcgaatag aaacccagcg gcgcggtcac cgtcagcccc
    11041 acgggcgcca ggtcgaagcc gcgcgccttg atctgggcat ccaggaaggg cttgtgctgg
    11101 aaggcattgg cgtccaggtc gccggcggcc agcgcggcat tgggcagttg gtagtcgttg
    11161 aagaccacca cgtcgatgtc caggccgtcg cgcttggcca cgcgcttgac gacattgaag
    11221 atctgctcgg cggagccgac cgagatgccg accttgatgg acgacttgtc ggcggccgat
    11281 gccgtgaaag aaacagccag cgccaggacg ggaacggaca gcgcgcgcaa caacttggac
    11341 atgaaacggg tttccttgca acagggggag gagacccgtg gattctgtca cccaccgcta
    11401 tatgccggaa aatacatagt cggcatgtcg atatggggca taaggaaatg actaaaacac
    11461 cttgccctcc agcaagtgcg tcgtcgtccc gttgaggtgg taatccccga ccacccgcgc
    11521 cttgtgcaac agcgggttgt ggctgaagag ggtgcgcagg ctgcgccagt ggcggtcgaa
    11581 gttgtgcttg cgcccggtca tcgaggcgcc gccggcgttg aagagctttt cggcggcatc
    11641 cagcgccagc tggctcacca ccagctgggt cttggccgtg accagcgcgc tctccagcac
    11701 caggtccgag gcgttgggcg catcggcctg gatggcgtcg gccgaacggt cc
//
LOCUS       DASWPO010000085        41592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2339027, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000085 DASWPO010000000
VERSION     DASWPO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 41592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41592
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339027"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /gene="fumC"
                     /locus_tag="VGC69_04995"
     CDS             <1..1199
                     /gene="fumC"
                     /locus_tag="VGC69_04995"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0294677.1"
                     /translation="ATIADAIVRAADEVIAGKWPDEFPLSVWQTGSGTQSNMNMNEVL
                     ANRASELLGGVRGEARKVHPNDHVNRGQSSNDTFPTAMHVAAAVEIERHLLPALKALR
                     ATLADKSAAYYDIVKIGRTHLQDATPLTLGQELSGHVAQLDLAEQQIRATLPGLHQLA
                     IGGTAVGTGLNAHPEFSAKVAAELAHDTGSPFVSAPNKFQALASHEALLFAHGALKAL
                     AAGLMKIANDVRWLASGPRSGLGEISIPENEPGSSIMPGKVNPTQCEAVTMLAAQVLG
                     NDVAINIGGASGNFELNVYKPLLIHNFLQSVRLLADGMASFDAHCASGIEPNRERIAE
                     LVERSLMLVTALNPHIGYDKSAQIAKKAHKEHTSLKEAALALGYLTEDEFNRWVVPAD
                     MTNAAR"
     gene            1371..2363
                     /locus_tag="VGC69_05000"
     CDS             1371..2363
                     /locus_tag="VGC69_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugE"
                     /protein_id="HEY0294678.1"
                     /translation="MIKTRPLVNSLALTGAAALLSGIAAPAQAAYPDHPIRVIVPFAP
                     GGSTDIIARLVTQRMSQELGHTMVIENKGGAGGAVGAAEAARAAPDGYTLSIATVSTM
                     AVNPACRPGDLPYDPIKDFQPVTNFANTANVVSVNPKEMPVKDFKEFLEVIKKNPGKL
                     SYGSSGTCGVLHLMGESFKMATGTSIVHVPYKGSGPAVADTVAGHIEMVFDNLPSSMP
                     QILSGNLHAMAIAWPERIPAIKDVPTFKEVGLPVLNQPVWYGLLAPKGTPMAIVNKLR
                     DAAVVALKDPKVIKALDEQGSAPSGNTPEEFAKEIKEQYDWAKDVVDKQHIKME"
     gene            complement(2350..3225)
                     /locus_tag="VGC69_05005"
     CDS             complement(2350..3225)
                     /locus_tag="VGC69_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294679.1"
                     /translation="MRLRHIEIFEAIRRTGSLTEAAATLHITQPAASKLLANAEAQLG
                     FKLFERVKGRLVATREAEILTPEIARISLDLNSVRKLAASLRERPHGHLRLGCAPALG
                     LGLLPRVIRASRDARPGLTFDIQTHHSAELVQGLLTRELDLAVTFDTHEYPGLVREEI
                     GHTELVNLGRKTGEEPVSLQDLCGEPLIVLDPRDAAGALLQMALDALGLEAPQALQVQ
                     THYVACALAEAGCGEAIVDAITARAMLRPGLRLRPITPALRVPISVMRRGHDIMSGLH
                     HELIDGLRRACVTPS"
     gene            3320..5068
                     /gene="ggt"
                     /locus_tag="VGC69_05010"
     CDS             3320..5068
                     /gene="ggt"
                     /locus_tag="VGC69_05010"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0294680.1"
                     /translation="MRALPRIVLPCLLLACAAIAQAKQPAPADANPEAATGYTAKPLA
                     HAKHFMLATANPLASGAGYQVLKQGGSATDAVIAAQLVLAVVEPQSSGLGGGAFMMHY
                     QADTGDVQVYDSRETAPAAARADRFLRGGKPIPFHEAVNNGRSVGTPGVLRGLELAHR
                     DHGRLPWKTLFGPAIALCEQGFAVSPRLHALLEEERVELLKQPAAAHHFFDADGRAWP
                     VGHVLKTPELAAVLHRVADEGPDAFYHGQIAHDIVAAVRGHAQPGDLSEADLAGYRAV
                     VRAPVCGRYRGYLLCGPPPPSSGPMTILQILGELEQYPMGRMQPESLQAVHYFAEAWS
                     LAYADRDFYEADPAFVNVPVDALIDPRYLRARGALIRPDRSMGEARPGDPAGMLQARG
                     KDDAFEVPSTSHLVAVDRDGNVVSMTTTIESAFGSKIMVHGFLLNNEMTDFSSSYQDA
                     QGRLVANRVEPGKRPRSAMSPMIVFRMGKPYLATGSPGGSTIINYVAKTLMAVLDWRL
                     DIQQAVALPNLGSRNHGVELEKGTALVKLEPALRAMGHEIVETDFPSGLQGIVIGPDG
                     LTGGADPRREGVALGD"
     gene            complement(5105..6121)
                     /locus_tag="VGC69_05015"
     CDS             complement(5105..6121)
                     /locus_tag="VGC69_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294681.1"
                     /translation="MPSFAIERPARPRTAFRRLLAVLAGCLALAAGGPAALAAAYPDH
                     PIRLIVPFAPGGGNDNVARTVGRVLSELLGQAVIVDNRAGAGGTLGAEAAARAAPDGY
                     TLFLGGVGSHAINPNLHTDLPYDPIKDFEPVALLARAPMVLVAHPRLHVRTVAELIAL
                     ARKEPGQLDYASNGTGSSSHLAAAMFAAMAGVNMVHVPYKGLSPALTDLISGTVPLMF
                     SSVVAIVPHIKDGSLVALGVTSTERLPVLPGVPTIAESGVPGYESSSWYGILVPAGTP
                     RPIVLTLNKALNQALQAPQVHDSLAGDGAQPAGGTPEEFGAYIKREKDRLGEVIRTAG
                     IHLD"
     gene            complement(6224..7078)
                     /gene="purU"
                     /locus_tag="VGC69_05020"
     CDS             complement(6224..7078)
                     /gene="purU"
                     /locus_tag="VGC69_05020"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY0294682.1"
                     /translation="MPHNDFILTLSCPDRTGIVYRVSGLLFDLGCNILDSQQFGDDET
                     GRFFLRVHFDLPAAAGEDGLRVKFGALAAGYAMDWHIHDARTKPRLLILVSRQGHCLN
                     DLLFRASSGQLHAEIAAVVSNHNDYAGLAASYGIPYHYLPVTPDTKAEQEQQVLALVE
                     RERIDLVVLARYMQILSTGMCRALEGRAINIHHSFLPSFKGARPYHQAHTRGVKIIGA
                     TAHYVTADLDEGPIIEQDIERVDHSMSAQQLTQVGSDVESLVLSRAVRWHVEHRILQN
                     GSKTVVFR"
     gene            complement(7092..8201)
                     /locus_tag="VGC69_05025"
     CDS             complement(7092..8201)
                     /locus_tag="VGC69_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0294683.1"
                     /translation="MSADVDCLVIGAGVVGLAVARALALQGREVLVAEAAEAIGTGTS
                     SRNSEVIHAGIYYPAGSLKARLCVRGRQLLYAYCAEHGVPHRRLGKLLVATSVEEAGL
                     LDGIAARARANGVDDLQKITGPQAAALEPALHCTAALASPSTGIVDSHALMLAYQGDA
                     ENAGAQCVFHTPLESARARPEGGFEARFGGGEAMALSCNVLVNAAGLHAPGLARRIGG
                     LPAASIPAEYLCKGSYFTLSGRAPFSRLIYPIPAHAGLGVHLTLDLGGQAKFGPDTEW
                     TDRADYTLDPRRADVFYAAVRRYWPGLPDGALAPGYTGIRPKISGPREPAADFDIAGP
                     AAHGVPGLVNLFGIESPGLTASLAVAEEVLRRLSP"
     gene            complement(8269..8781)
                     /locus_tag="VGC69_05030"
     CDS             complement(8269..8781)
                     /locus_tag="VGC69_05030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0294684.1"
                     /translation="MSFSPSFDASHFRTTLGHFATGVTLITTQAADGSPVGLTVSSFN
                     SVSLNPPLVLWSLGMSSSSLAAMQQCERYVIHVLSAEQIALARRFATGTAAERFTGLA
                     LARAPSGTLMLAGESAAWFECRNRSRYQEGDHYIMVGEVEHCGYSDAPPLVFHAGGFD
                     LTPRQQPLES"
     gene            complement(8795..9424)
                     /gene="pdxH"
                     /locus_tag="VGC69_05035"
     CDS             complement(8795..9424)
                     /gene="pdxH"
                     /locus_tag="VGC69_05035"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY0294685.1"
                     /translation="MPLSDLRQSYEKDVLLESQAAASPIEQFQRWFDEAMAAQAPEPN
                     AMTLATVDADGQPSARIVLVKNVDERGFTFFTNYESRKGEAIAADPRAALLFFWQPLE
                     RQVRIEGRVQKVSAEESDRYFHSRPPGSRIGAWASEQSRPTTRDALEARERAFRERYG
                     DQPPRPPHWGGYRLVPTAFEFWQGRPSRLHDRLRYLPDGQGWKIDRLSP"
     gene            complement(9509..10078)
                     /locus_tag="VGC69_05040"
     CDS             complement(9509..10078)
                     /locus_tag="VGC69_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY0294686.1"
                     /translation="MEASLRHWRSGEDVWVYGYGSLIWRPEFTYVERRLATLRGHHRA
                     LCLWSRVNRGTPECPGLVFGLDRGGSCRGVVFRLTGAEVPTHFPKLWEREMSSGAYLP
                     RWLNCETGQGMVKALVFVMNRDNPAYIRTLPDAELVAIVRRAAGRYGPCTDYVMETVR
                     ALRASGICDARLETIACHLERPGEPLPDE"
     gene            complement(10156..11310)
                     /locus_tag="VGC69_05045"
     CDS             complement(10156..11310)
                     /locus_tag="VGC69_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294687.1"
                     /translation="MLNHVALAGGRITTSLTALKLGSSTLEVGTLVAIFAVMPMLLSV
                     HAGRWIDHVGARRPLVIGSTLLCLGALLPFLFQTRSALLAAAFSISIGFMLNQMAVQD
                     LLGTADPTRRLRNFSWLALSQSVSGFSGPLIAGLSLDHLGSRMAFGLLALAPLASAAG
                     LYLLRRPLRDTNAALSSGDLRSRAGKQGKMLELLATPTLRQVLLVNTLLSSAWDTHLF
                     VVPIYGTQIGLSATTIGVILAAFSAATFVIRLFLPMIQKHVQPWNLVRATIITTATDF
                     SIYPFFSESTTLAGLSFVLGLALGSCQPSMLSLLHHHAPPGRAAEASGLRLAFISTSQ
                     VCLPLTFGALGAVIGIMPLFWAYAACMALGGWANRRPPQPATLLTSATPS"
     gene            11510..12322
                     /locus_tag="VGC69_05050"
     CDS             11510..12322
                     /locus_tag="VGC69_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEY0294688.1"
                     /translation="MNEMPVSSEIDADRRFGGLARLYGPGSPERLRAAHVAVAGLGGV
                     GSWAAEALARCGVGALTLVDLDHIAESNVNRQIHALTPTLGQAKIEAMAQRIRDINPD
                     CAVTRVDDFVEPGNVAEVLAGDYAALIDCTDQAAAKIAMILHARARGVPFVLCGGAGG
                     KTDPLALRAGDLSEAVNDALLAKLRNTLRREHGFPRGSDQNGKALKRVPKMGVRALWF
                     DQPAILPDAWAQSGSDERSAPQGLSCAGYGSVVMVTAAMGMAAANEALRRIL"
     gene            complement(12709..13020)
                     /locus_tag="VGC69_05055"
     CDS             complement(12709..13020)
                     /locus_tag="VGC69_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY0294689.1"
                     /translation="MQWIKTMAQDDVTDDDVTPAEAGEREIALYKVEGEIYATDRMCT
                     HGAASLCDGFLMGHEIECPLHQGRFDVRTGKALCDPLTEGVAVYPVRIEDGHVFVGVE
                     G"
     gene            complement(13033..13503)
                     /locus_tag="VGC69_05060"
     CDS             complement(13033..13503)
                     /locus_tag="VGC69_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY0294690.1"
                     /translation="MDFDLYYRIARLYGDYAAALDAAEWDKWPGFFLEDCTYKVIPRE
                     NYDRGYPLATLSFESRGMLKDRIYGTTGTIFHDPYYQRHVVGAPRILSARDGRVEAEA
                     NYAVFRTKPGQPSMVFNVGRYLDVIRDTADGLKFESRLCVFDSELIPNSLIHPI"
     gene            complement(13508..14752)
                     /locus_tag="VGC69_05065"
     CDS             complement(13508..14752)
                     /locus_tag="VGC69_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0294691.1"
                     /translation="MPDTPLPAWPESGSNRIPFGVYTSEALHQRELERLFYRAHWCYV
                     GLTAEIPRPGDFKRTVVGERSVILARDKDGEIRVVENVCAHRGMQFCREPYGNRTDLV
                     CPYHQWTYDLRGNLIGVPFRRGVKQDGKVNGGMPPDFKTEDHGLTKLKVAVRNGVVFA
                     SFDHGVEPLEDYLGPAILPYFDRVFDGRELVIHGYSRQRIPGNWKLMQENIKDPYHPG
                     LLHTWFVTFGLWRADNRSELRMDSHGRHAAMISTRGQGGKGAVTSGVTSFKEQMQLND
                     PRFLDIVTEPWWQGPTAVLMTLFPSVIIQQQVNSLSTRHIQPVGHDAFDFVWTHFGFA
                     EDTPEMTQRRLRQANLFGPAGFVSADDGEVIEFSQQGFEQKPWHRCVAELGGKTVENT
                     DHMVTETLIRGMYRYWRGVMEG"
     gene            14916..15818
                     /locus_tag="VGC69_05070"
     CDS             14916..15818
                     /locus_tag="VGC69_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294692.1"
                     /translation="MNLHELDLNLLVVFDALHRHGRVSLAAQSLGVSQPGVSNALRRL
                     RKLLGDELFLRTSGGMVPTPYAQSLAQPISDALASLHATLNAHASFDPGRSERNFVIG
                     VNDVGELYFLPRLMRALGRDAPGVTVRAELTGGVDVKEEMERGRIDLVLGFLPGLKSG
                     FFQRRLFRQPYVCIFRQDHPLAGTGVTLKQFQAAEHAAVVSGGTGHGVVDEIINRFGV
                     RRRVKLTVPHFMALGPLLQATSLIAVVPRRFADAACAPFGLATAACPVKIPESVINVF
                     WHARNHREAANQWLRKRVVEEFAD"
     gene            15891..16859
                     /locus_tag="VGC69_05075"
     CDS             15891..16859
                     /locus_tag="VGC69_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294693.1"
                     /translation="MQRKLMAALALSAAAAYSFSAQAADTWPSKPLTIVVPFAPGGAN
                     DLIARLYAKQIGVELKADVIVENRPGAGAVVGTAHVARSQPDGYTFLVASNGTVTNTL
                     IRSDQPFKDDEIEPVAMLAIVPSIIVTNPANPAKDLKELVENAKKNSSRPVTFSTAGM
                     GSTPDFVGAMVKLETGVNMQTIPYKSGAENVAAVVGGQTDMTSEADMVTLPLVKAGKL
                     KALATTWDHLAVAPDIKTTAELGFPNIRIGHWEGLYVRAGTPADIMDKVNAAVLRASK
                     SKEILDVLNAYSVVPGTMTRPEFVKFTQDERVRLGKVVKGANMQPD"
     gene            complement(17268..23120)
                     /locus_tag="VGC69_05080"
     CDS             complement(17268..23120)
                     /locus_tag="VGC69_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEY0294694.1"
                     /translation="MSSEIRIVGARQNNLKNLDLTLATGELIVVTGVSGSGKSSLAFD
                     TLYAEGQRRYVETFSPYARQFLDRMDKPQVDRIEGILPAIAIDQTNPVRSSRSTVGTM
                     TELNDHLKLLYARGATLYCRGCGRPVRRDTPDTIRAAIAERTSTVAPTLRPAGLLPPE
                     GARPPWGGPAADGPGDPRLVVTFPLGVPANFTEEEVKGFLDQQGYTRVHSEEAPRAGA
                     ASHGAKAGGKTKGKGKKAAAPAVEMQRILHVVQDRFRFSSAEPERVMEALDTALRMGA
                     GHLSIHAMDDEGKDASVWKFSNRLHCADCDIEYSDPLPSSFSFNSPLGACDACRGFGR
                     VIGIDLGLVIPDENKTLLEGAIKPWQTPSFKECQTELEKYAPRAGIPLSVPWKSLSAE
                     QRAWVLDGTPDWKGGSQAWKTQWYGVHRFFQWLETKAYKMHVRVLLSKYRSYTPCPVC
                     KGARLKPDALLWRLGSREDADDVLPGEGAHEGRYRRFMPASASWTREQLGAMAGLSIH
                     DMMLLPIERVKRFFDGLRFEGALDAASDLLLTEARARLKFLCDVGLGYLTLDRQSRTL
                     SGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMHRVVEVMHRLRDAGNTLVVVE
                     HDPQVMVAADRIIDIGPGPGERGGNIVFDGTPEQLRVAPTLTGDYLGGRLRVEAPRPM
                     PVAASTPRLILEGARAHNLRNVSVELPLGRLVCVTGVSGSGKSTLVQDVLHPALLKYL
                     GKPTETPGEYDKLLGAEQLADVVMVDQTPIGKTARSNPASYVGAFDAIRKLYAQAPLA
                     RERGYMPGTFSFNSGDGRCPTCGGTGFEHVEMQFLSDVYLRCPDCDGKRFRPEVLEVR
                     VEHLGRSASIDQVLDMTVSEALDFFKGLRDVQTGLAPLADVGLEYVRLGQPVPTLSGG
                     EAQRLKLAGHLAEAARSGISTARVAKKGSLFLFDEPTTGLHFDDVARLMRAFRKLLAA
                     GHTLLVIEHNLDVIRAADWLIDLGPDGGDAGGEVVGVGTPQDLMDNPRSYTGQALRDY
                     AVSILPVEAASAPAPALAEPTVAYGAENGTGSGTPLQSLMRRKRKGAPAIEILNAREH
                     NLKNVSVEIPRDKFTVITGVSGSGKSTLAFDILFNEGQRRYLESLNAYARAIVQPAGK
                     PDVDAIFGIPPTVAIEQRTSRGGRKSTVATMTEIHHFLRLLYVKLGTQYCPDDHVPVE
                     PQTSDQIVARLLRDHKGVHIGLLAPLVTARKGYYTDLAKWAGAKGHTHLRVDGEFIPV
                     SPWPRLDRYKEHTIELPVADVVVDPANEADLRAAVKSALENGQGVMSAVWPVDTLHES
                     LSSELQQQHFSTKRACPTCGKSFPEPDPRLFSYNSKHGWCTACFGTGLQLQGFDAEQT
                     GEETAWNAWYEGEAKVCGVCHGQRLNRTALAVRWRDRSIAQLAALPVSDAHTFFTGLV
                     TRGREGEIARDILTEIRGRLTFMQEVGLDYLALDRAAPTLSGGEAQRIRLAAQLGSNL
                     QGVCYVLDEPTIGLHPRDNRILLDALARLEGNGNTLVVVEHDEDTIRRASHLIDIGPG
                     AGVRGGRVVAQGTEQDLIDAPESITGRYLAHPLAHPLNGRRAVASDTPMIQVRGARLH
                     NLRKVDADIPIGRLTVVTGVSGSGKSTLAREVLLDNLTHAVSQGKAPGWSGCERIEGW
                     DLIDRVLEVDQTPIGKTPRSCPATYVGFWDDVRKLYADAREARMRGWTAARFSFNTGE
                     GRCPVCEGQGMRTIEMSFLPDVKVPCDGCNGARFNSDTLGVKLREKSAGDVLGMEVDD
                     AIGYFAAHPKIQRPLQLMQDVGLGYLTLGQPSPTLSGGEAQRIKLVTELAKARLGTAE
                     GVFKTGRAGRATSHTLYVLDEPTVGLSMADVEKLIHVLHRLVDAGNTVVVIEHNLDVI
                     AEADWLIDMGPEGGDGGGRLVAQGAPEAVMKRADKSHTGRILREFLSARS"
     gene            23340..24011
                     /locus_tag="VGC69_05085"
     CDS             23340..24011
                     /locus_tag="VGC69_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0294695.1"
                     /translation="MVYTLDFSTVFKSYDVLLAGAWLTIELSAAAMALGLIVAVFGAL
                     AKLFGPGWLKALVDAYIEIIRNTPFLIQIFLVYFGLPLAGLRLEPVPAALLAMVVNVG
                     AYAIEIVRAGIDAIPRGQISAGFALGLGRLQVFRYVMIRPALRKVFPALTSQFIWLML
                     GSSVVSAISADDLTQVASNLASSNFKHFEIYIVVTGMYLVMTLLFSGVFALIRRWAFS
                     YPEGR"
     gene            24098..24754
                     /locus_tag="VGC69_05090"
     CDS             24098..24754
                     /locus_tag="VGC69_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0294696.1"
                     /translation="MRPFGFSEFLFIVEAVRWTVLLSLVAFVGGAVVGLPVALARTAP
                     PRWLRVCATVYIQLLMGTPLLMQLFLMYFGPPALLGVEVDAWVAAAIALTLNSAAFLA
                     EIWRGCIQAVPQGQVQAARALGLRYPSLMRCVVLPQAFRISLPATVGFLVQLVKATSL
                     ASIIGFVEVTRAGQIINNATFRPAMVFGIVAIIYFVLCWPMSRYAAALERRYSAAQAR
                     "
     gene            24816..25625
                     /locus_tag="VGC69_05095"
     CDS             24816..25625
                     /locus_tag="VGC69_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294697.1"
                     /translation="MLMQTVRQLGRAAAVTALLAGSLALAPSTQAQTVDQIKSKGTIT
                     VGVMVDFPPFGLLDKDNKPDGYDEDIARLLAKDLGVKLDFVPVSGPNRVPYLLTGKVD
                     LIIGALGITPERAKQVQFSDIYATTKLAVYGQKGVKVESEQDLKNVRIGVARASTQDM
                     AITKRAPEGTRIQRYDDDASAVQALLAGQVQVIGLTTTLIPELDKIAPKGAYEIKLPL
                     DTFSYGIAMRPGQDDLLKGVDAFLAKIKQDGTLNQLHRKWLGTDFVEPGKS"
     gene            25657..26466
                     /locus_tag="VGC69_05100"
     CDS             25657..26466
                     /locus_tag="VGC69_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0294698.1"
                     /translation="MVSKPASAVAGTAAFSKFMDVLERVAEAEQPPGVAELARASGYP
                     RPTVYRIVSALLEHGLISQRREGGAYELGPRLLSLASRSWSRFDLRVALEPELRALRD
                     ATGETVHMAVPSEGAMIYVEKIESPGPLRMVSRVGGRLSLHSSAVGKAYLAALPEQEC
                     EALLDRLPMPGFMPNTITTQPAMRAELQRVREQGYAVDNEENEAGIVCYGVVLCDSGG
                     NPVAGVSVSTLLFRQQRDPRAAYVEPLLRLQAAAARTLYAGPVQAGAGKMP"
     gene            26486..27430
                     /locus_tag="VGC69_05105"
     CDS             26486..27430
                     /locus_tag="VGC69_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY0294699.1"
                     /translation="MNQPDCAAFRYDVAGLGEALIEFNQTRAGRPDYLQGFGGDTCNA
                     VIAAVRQGARGAYLTRVGGDAFGQQIVDLLRAEGVDAAGVVVDPDTYTGMYFVQHGPD
                     GHVFSYLRGDSAAARMQPAMLGHGLVERARFLHVSGISLAISASACDTVFAAIARARA
                     AGTQVSLDSNLRLRLWPLDRARAILREALCLADLFLPSLDDMRHLTGHEDPQATLDWI
                     RQAGARGVVVLKLGKDGAVLDDGATRTRVPGHVVRAIDATGAGDCFAGSLLARRCQGD
                     GWLAAVRYANAAAALSTLGWGAVAPIPTPAQVKAALAD"
     gene            27546..27674
                     /locus_tag="VGC69_05110"
     CDS             27546..27674
                     /locus_tag="VGC69_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294700.1"
                     /translation="MAERKRVRRNTLERRCLGKAFKRLFVKSPKGVFKMLEKVKRV"
     gene            27901..28011
                     /locus_tag="VGC69_05115"
     CDS             27901..28011
                     /locus_tag="VGC69_05115"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294701.1"
                     /translation="MYVAKFSDTVYVLHAFRKKHGRHRKPIWTLPLSAIS"
     gene            28025..28336
                     /locus_tag="VGC69_05120"
     CDS             28025..28336
                     /locus_tag="VGC69_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0294702.1"
                     /translation="MKKQTDPRWDDESVTRGSGNVFIDLGFDPAEAEVMRMRAEVMIH
                     MRDKLKEYGWTQAEAARRLGITQPRVSRLVKGKVEDFSLDMLLVLAGRVGLRPELRLS
                     A"
     gene            complement(28351..29127)
                     /locus_tag="VGC69_05125"
     CDS             complement(28351..29127)
                     /locus_tag="VGC69_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0294703.1"
                     /translation="MHADNSAYLKPAHIPDEEWQLRVQLADFYHLVDYLGWTEMIFNH
                     ISARLPGPANHYLVNPFGLNYYEVTPENLLKVGLEGDLVEPSPYPANPAGFALHSAIH
                     GARPDVHCIAHTHTNPVSAITLKQDGFHHDDFYGAQLFGRVGYHDFEGITLFADEKTR
                     MLASLGDKHVLALRNHGVAVCEKDIPSTFMLLWTVQRAAEIQYHAGCLPGSNTSIADI
                     IKTRCSALAAGLIRDDSFAIKLFDATVRKMRRAKQLGICV"
     gene            complement(29159..30133)
                     /locus_tag="VGC69_05130"
     CDS             complement(29159..30133)
                     /locus_tag="VGC69_05130"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0294704.1"
                     /translation="MRICIAGAGAIGGALAARLAHAGQDVSVLARGATLAALRADGLT
                     LRDLSGEVRARVPASDTADFGPQDAIFLCTKTHHLAEMLALCAPMIGPDTVIVPAVNG
                     IPWWYFHAEGGPHAGKPVAAVDPDGALLAVTPLAQLVGCVVYMTAETPKPGTVLSNSP
                     HRLVLGEPGGGLSPRVEQLCQALTGAGIETQASPRIRDGIWSKVAANLSSNPLSVAAG
                     GATLGQIYGQEPLRSLAENVIRETMAVAQAYGAQLANDPATMVERAAKLGDFRTSMLQ
                     DFHAGRPLELAAIGDAVLELAERYDIPMPTTRSILSITRFRDARRSGY"
     gene            complement(30210..30941)
                     /locus_tag="VGC69_05135"
     CDS             complement(30210..30941)
                     /locus_tag="VGC69_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294705.1"
                     /translation="MSKKLLEVRDLQVAYGKVTAVAGVNIDVAEGQIVTVIGPNGAGK
                     TTLLSAIMGVLPSRGAITFGGQTREHAEIEEMVAAGMGLVPEKRELFSEMTVEDNLVL
                     GGFHRYRMGDRDQGISLREVYDLFPRLQERRAQLAGTLSGGERQMLAVGRALMAKPKL
                     LMLDEPSLGLAPRIVREVLRIVSELRKLGVSILLVEQNARAALQVADYAYVLETGAVT
                     LEGPAAELARDPRVIEVYLGMGGKA"
     gene            complement(30934..32739)
                     /locus_tag="VGC69_05140"
     CDS             complement(30934..32739)
                     /locus_tag="VGC69_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY0294706.1"
                     /translation="MKLSPRHLIALFVVLLALGPLLLPPFYVTLLDYIGLYALVAIGL
                     VLLTGVGGLTSFGQAAFVGIGAYTSTVLCLHAGDLPGGLAWLGTPWAGLVAGMLLTLV
                     VAYVLGAITLRLSGHFLPLGTLAWGLSLFYAFSTVDGLGGYTGLTDIPPVSLFGWKLT
                     QGGQIYYLIWLFLLLGVWTTQNLLDSRMGRAIRALRGGRVMAESMGVDTAQARMVIFV
                     IAALQACASGWLYAHFQRFINPYPFSVEQGIEYLFMAVIGGVGYVWGAVLGAGIFTIL
                     KEWLQDLLPRLLGHTGNFETIVFGFGMVLLLHRARTGLWAALSHRLPIRAAQRQVDMK
                     AEALPRRPMPPRGEVVLQAREITRRFGGLVANNKMTLDIHAGEILALIGPNGAGKSTM
                     FNQISGVDTPTSGTVTLRGQAVTGIGARAIAKLGLSRTFQHVRLLGRMSVLENVAIGA
                     HLRGTSGILRSAWRRDRAEEARLLAEAARQVERVGLGQFMHTPAGSLALGQQRILEIA
                     RALAADPCLLLLDEPAAGLRHQEKQELAQLLSRLRSEGMAVLLVEHDMDFVMNLVDRI
                     VVMDFGEQIAEGLPADIQNNPAVLEAYLGGMDENE"
     gene            complement(32736..33782)
                     /locus_tag="VGC69_05145"
     CDS             complement(32736..33782)
                     /locus_tag="VGC69_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0294707.1"
                     /translation="MNAQIALLLGQDGLTNGAIYALLALAILLVFTVTRVLFIPQGEF
                     ASYSALSMAAIQAGKPVPLVWLLAAFAVCEALADLWAMHRRTGRIHLRMPLLFRLAYP
                     FVLAVLFYTLPLANLPMSVQALLTLALVVPMGPQIYRLFFQPIATASPLVLLIVSIAL
                     HLALLGLGLLAFGAEGGRTTPFIDATLSLGDLRINGQAACVVLVSILLIIALYLFFDR
                     SMYGKALRAVAYNRIGARLMGISPPFAGKITFLFAALIGAISGLLIAPITTLYFDSGF
                     LLGLKGFVGAIIGGLVSYPVAAAGAVLVGLIESFSSFYASAYKEIIVFTLIIPVLLWR
                     SLKSRHVEEEEDGE"
     gene            complement(33801..34949)
                     /locus_tag="VGC69_05150"
     CDS             complement(33801..34949)
                     /locus_tag="VGC69_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294708.1"
                     /translation="MKCTKLTLAAAAVAALFTLPAMAQVKVGVITSATGPTALVGIPQ
                     KNSVPLLPTKIGDLSVEYISLDDGSDTTASVTDIKKLISEEHVDAIIGPTGTPNAMGV
                     IQFVAEAGVPMLAPVGGADVVLPMTEQKKWVFKTTQNDDIIAKALVDQMAKTGIKTLG
                     FIGLSDPFGENWYKVVSGLADKAGIKIVANERFTRYDNSVTGQSLKILAANPQAVLVA
                     APGGPSILPEKTLVDQGYKGQFYQTHGAALDAFLKQGGKQVEGTILAASLMLVLPEIP
                     DSNPSKAVAAKYIADYKKLYGNEPATFGANVYDAGLLLEKAIPIAEKSAKPGTKEFRS
                     ALRDALEQTKDLVGTQGVYNMTPQDHSGFDERGRELITVKNGAWTLLK"
     gene            complement(35006..35794)
                     /locus_tag="VGC69_05155"
     CDS             complement(35006..35794)
                     /locus_tag="VGC69_05155"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0294709.1"
                     /translation="MSAKILAQFMGALASGGIRIIDLTETLTPEFPTIVLPPEFGQAW
                     PFRIEEISRYDERGPAWYWNNFSCSEHTGTHFDAPVHWVSGKDQPDNTVDTIPLSAFV
                     ASACVIDCSEPSGQDPDFLLGIDYVKQWEEQHGKIPDGAWVLMRTDWSKREKSVDYLN
                     MQADGQHTPGPDAEVVPWLIKERNVHGFGTESVGTDAGQAAHLDPPLPCHYFMHGNNR
                     YGLQCLTNLDQLPPTGAVIFSAPLKIRHGSGSPLRVLALVPGKP"
     gene            complement(35904..37145)
                     /locus_tag="VGC69_05160"
     CDS             complement(35904..37145)
                     /locus_tag="VGC69_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294710.1"
                     /translation="MTEALRQPPKKDPVARSRQPTRPPGARSRSALGLTAAAAEGRFE
                     LQQCAECGTVQYPPREVCVHCLSEHLPWKPVSPKGRLLVSTTLHHSNDLYFRERLPWR
                     IGTVRMEAGPSVVAHVHGDLADGDEVRLAIKLDRSGQAVMLALPARNTPHMEDDKILR
                     ETSCDPKFRRALITDGKSAVGLAVAQALLDAGASQVYIGDPQRWRKEAAFDAFAADPR
                     VSVIPLDVTDTDSLDRAAGEFGGKVEILVNTADMEREGGLLHTKDVNVARNLLDINVL
                     GMMRLAHAFGPAMCARAADGARNAVAWVNVLSIYAQMSLPSRAIWSASKAAALSLAQG
                     LRAEMRPSGVRVVNVFPGPLDHEWEQLTPPPRVAPKAVAQAIVGALKDGLEEVYVGDV
                     AKEFRARLAENPKGLERELGA"
     gene            complement(37219..38388)
                     /locus_tag="VGC69_05165"
     CDS             complement(37219..38388)
                     /locus_tag="VGC69_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0294711.1"
                     /translation="MASRLSYDGVVAALPVTIPYERYSTHAAHWWLGRALGGLVRQSG
                     LPKDAIDGVCVSSFTLAPDTAVGLMQHLGMSPRWLDHIPMGGASGVVGLRRAARAVQA
                     GDASVVACISGDANHVDSFRNTVSQFSRFAQDGVYPYGAGGPNASFALLTTHYMRTYG
                     ATREDFGKLCVAQRDNALRYPHALMKKPLTLQQYMDARVITDPIHLFDCVMPCAGAEG
                     FLVMEEKRARDLGLPYARILGTIERHNAWHDDPIQTRGGWTLDIDELYAQAGATPADL
                     DFMQAYDDYPVINFMQIEDLGLCAKGEAAEYVRRNTFTVDGTLPFNTNGGQLSVGQAG
                     AAGGYLGMVEALRQLSNTAGRTQVADARVGLVSGFGMINYDRGLCTAAAILGSAQ"
     gene            complement(38388..40022)
                     /locus_tag="VGC69_05170"
     CDS             complement(38388..40022)
                     /locus_tag="VGC69_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0294712.1"
                     /translation="MSAKTVYEAFRDTTAKWGKNPFLCTLPETAEAYGIEAGELSYDD
                     AFATIERLRGAYAVAGYGHGHRVGLLLENRPAFFLHWFALNALGVSVVPINIDLRAAE
                     LEYLIGHSELILAVALPGRHASLQEAAQRAGRALRTMTPDEAPPPALTAAPLAGSPDD
                     RTECALLYTSGTTGRPKGCILPNEYFLHCGRWYANIGGLCTLRAGVERMLTPLPLVHM
                     NAMACSTLAMVVTGGCLIILDRFHPKTWWDSVRRSRATVLHYLGVMPAILMKAAPGPG
                     DRDHAIRFGFGAGVERSLHAEFENRFGFPLLEAWAMTETGNGAVIIASKEPRFVGTSS
                     IGTQEPDVEVRIVADSGAEAAPGEPGELLVRHAGAKPQFGFFAGYLKDPEATAEVWAG
                     GWFHTGDIVRRDADGMIRFVDRKKNVIRRSGENISAVEVETILLTHPLVKAVAVAAVP
                     DAIRGDEVLACIVPETTPASREAMEAAARELVTWSLGELAYYKVPGYVAFVDALPLTP
                     TNKIQRGEMKKLAPELVGQPACVDTTALKKRQEGGH"
     gene            complement(40049..40537)
                     /locus_tag="VGC69_05175"
     CDS             complement(40049..40537)
                     /locus_tag="VGC69_05175"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY0294713.1"
                     /translation="MMTDPQLIAFIYQEARLIDEQKFEDWLDLYDDDAWYWMPLTHGQ
                     PDARLHTSLMHENKLLLRVRVERLAGQRTFSQQPKSRCHHLLQTPFVEHGHAEHQPAE
                     GRYVTRTAFHYVETRNDEQTLYAGWATHHLVETGGALKIRFKQVDLVNCEAAFGNIQL
                     LM"
     gene            complement(40534..>41592)
                     /locus_tag="VGC69_05180"
     CDS             complement(40534..>41592)
                     /locus_tag="VGC69_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0294714.1"
                     /translation="KGVQVAADGSGNTGKFFRCPYHAWTFKTDGSLLSVPLKKGYENT
                     GFEQCEASRGMAAVSAVRNYRGFVFCRLSETGESFEDFFGESLSTIDNMVDRSPVGKL
                     EVAGGVLRYMHRCNWKMLVDNQSDTCHPMVAHESSAGTAVRVWQEAPEGTPKPMAVEL
                     FAPFMSPYEFYENTGIRVWKNGHGHTGVSGSIHSNYSNIPGYWDSMVAAYGEERAHQI
                     LGESRHNTTFFPHIMVKGPIQTLRVFKPLAADRTLVESWTFRLVGAPDMLLERTIMYN
                     RLINAPTSMVGHDDLEMYERAQQGLTRTRAREWVNVARLYEPGEAGRENVVTNGTNEW
                     QMRNQYRAWARYMTEGAQ"
BASE COUNT         6430 a        13364 c        15033 g         6765 t
ORIGIN      
        1 acgcgacgat cgccgacgcc atcgtccgcg cggccgacga agtcatcgcc ggcaagtggc
       61 cggacgagtt tccgctgtcg gtctggcaga cgggctcggg cacgcaaagc aatatgaaca
      121 tgaacgaggt gctggccaac cgcgcctcgg aactgctggg cggcgtgcgc ggcgaggccc
      181 gcaaggtgca ccccaacgac catgtcaacc gcgggcagtc gtccaacgac acctttccca
      241 cggccatgca cgtggcggcg gcggtcgaga tcgagcgcca cctgctgccg gcgctcaagg
      301 cgctgcgcgc cacgctggcc gacaagagcg cggcctacta cgacatcgtg aaaatcggcc
      361 gcacgcacct gcaggacgcc acgccgctca cgttgggcca ggaactgtcc ggccacgtgg
      421 cccagctcga cctggccgag cagcagatcc gcgcgaccct gcccggcctg catcaattgg
      481 ccatcggcgg cacggccgtg ggcaccggcc tgaacgcgca tcccgaattc agcgccaagg
      541 tggccgccga gctggcgcac gatacgggca gccctttcgt gtcggcgccc aacaagttcc
      601 aggcgctggc ctcgcacgag gccctgctgt tcgcgcacgg cgcgctcaag gccctggcgg
      661 ccggcctgat gaagatcgcc aacgacgtgc gctggctggc cagcggcccg cgctcgggcc
      721 tgggcgagat cagcatcccc gagaacgagc ccggcagctc catcatgccg ggcaaggtca
      781 acccgacgca gtgcgaggcg gtcaccatgc tggccgcgca ggtcctgggc aacgacgtgg
      841 ccatcaatat cggcggggcc agcggcaact tcgaactcaa tgtctacaag ccgctgctga
      901 tccacaactt cctgcagtcg gtgcggctgc tcgccgacgg catggccagt ttcgacgcgc
      961 attgcgcgtc cggcatcgaa cccaaccgcg agcgcatcgc cgagctcgtc gagcgctcgc
     1021 tcatgctggt gacggcgctc aatccgcaca tcggctacga caagtccgcg cagatcgcca
     1081 agaaggcgca caaggagcac acctcgctga aagaggcggc gctggcgctg ggctacctga
     1141 ccgaggacga gttcaatcgc tgggtcgtgc cggccgacat gaccaacgcg gcgcgctgac
     1201 cggccgcgcc aaggacatgg gcagcacggc aaagtgctgc tatcctccgg acaggaccgg
     1261 cgatgcgcca cgaacggcat cgccggtcct gtgagtaagc cgtttgaagc aaaccacata
     1321 aacatgacgg catgcgccat ccgcatgacc gacttcagga gacatcgtcc atgatcaaga
     1381 cccgacccct ggtcaattcg ctcgcattga ccggcgccgc cgcattgctg tccggcatcg
     1441 ccgcgcctgc ccaggctgcc tatcccgacc atcccatccg cgtcatcgtg ccgtttgcgc
     1501 cgggcggctc caccgacatc atcgcgcgcc tggtcacgca gcgcatgtcc caggagctgg
     1561 gccataccat ggtgatcgag aacaagggcg gcgcgggcgg cgcggtgggc gcggccgaag
     1621 cggcgcgcgc cgcgccggac ggctacacgc tgtccatcgc caccgtgtcc accatggcgg
     1681 tgaacccggc ctgccgtccc ggcgacctgc cctacgatcc gatcaaggat ttccagcccg
     1741 tcaccaactt cgccaacacg gccaacgtgg tgtcggtcaa ccccaaggaa atgcccgtca
     1801 aggacttcaa ggaattcctc gaagtcatca agaagaaccc cggcaagctc tcctacggca
     1861 gctcgggcac ctgcggcgtg ctgcatctga tgggcgagtc gttcaagatg gccaccggca
     1921 caagcatcgt gcacgtgccc tacaagggct cgggcccggc cgtggccgac acggtggccg
     1981 gccatatcga gatggtcttc gacaacctgc cttcgtccat gccgcagatc ctgtcgggca
     2041 acctgcacgc catggccatt gcctggcccg agcgcatccc cgccatcaag gacgtgccca
     2101 ccttcaagga ggtcggcctg ccggtgctga accagcccgt ctggtatggc ctgctcgcgc
     2161 ccaagggcac gccgatggcc atcgtcaaca agctgcgcga tgccgccgtg gtcgccctga
     2221 aggaccccaa ggtcatcaag gcgctcgacg agcagggctc ggcgccgtcg ggcaacacgc
     2281 ccgaggaatt cgccaaggaa atcaaggagc agtacgactg ggcgaaggac gtggtggaca
     2341 aacagcacat caagatggag tgacgcaggc gcggcgcagc ccgtcgatca gctcgtggtg
     2401 cagccccgac atgatgtcgt ggccgcgccg catcacgctg atgggcacgc gcagcgcggg
     2461 cgtgatgggc cgcagccgca ggcccggacg cagcatggcg cgggcggtga tggcgtcgac
     2521 gatggcttcg ccgcaacccg cctcggccag ggcgcaggcc acatagtggg tctgcacctg
     2581 cagggcctgc ggcgcttcca ggcccagggc gtccagggcc atctgcagca aggcgccggc
     2641 ggcatcgcgc gggtcgagca cgatcagcgg ttcgccgcac aggtcttgca ggctgaccgg
     2701 ctcctcgccg gtttttcttc ccaggttgac cagttcggta tggccgatct cctcgcgcac
     2761 cagcccgggg tattcgtgcg tgtcgaaggt cacggccagg tccagttccc gcgtgagcag
     2821 gccctgcacc agctcggcgc tgtgatgcgt ctggatgtcg aaggtcaggc cgggacgcgc
     2881 gtcgcggctg gcgcggatga cccgcggcaa gaggcccagt cccagcgccg gcgcgcaacc
     2941 cagccgcagg tggccgtgcg gccgctcgcg caggctggcg gccagcttgc gcacgctgtt
     3001 gaggtccagg ctgatgcggg cgatctcggg ggtgaggatc tcggcctcgc gggtggccac
     3061 cagccggccc ttgacgcgtt cgaacagctt gaagcccagc tgcgcttcgg cattggccag
     3121 cagcttgctg gcggccggct gcgtgatgtg cagcgtcgcg gcggcctcgg tcagcgagcc
     3181 ggtgcggcgg atggcttcga agatttcgat gtggcgcagg cgcatggggc ggggagtccg
     3241 ggccttgggg aaagcctgat tctatgccgg ggggccggct tgtctatgat gggcgggttc
     3301 aatgccaagg aagcatgcaa tgcgcgcact cccacgaatc gtcctgccct gcctcttgct
     3361 ggcttgcgcg gccattgcgc aggccaagca gccggcgccc gcggacgcca accccgaagc
     3421 cgccaccggc tataccgcca agccattggc ccacgccaag catttcatgc tggccaccgc
     3481 caaccccctg gccagcgggg cgggctacca ggtgttgaag cagggcggca gcgccaccga
     3541 tgcggtgatc gccgcgcagc tggtgctggc cgtggtcgag ccgcaatcgt cggggctggg
     3601 gggcggcgcc ttcatgatgc actaccaggc cgacaccggc gacgtgcagg tctacgacag
     3661 ccgcgagacc gcgccggccg cggcccgggc cgaccgcttc ctgcgcggcg gcaagcccat
     3721 tcctttccac gaggcggtca acaacggccg ttcggtgggc acgcccggcg tgctgcgcgg
     3781 cctggaactg gcgcatcggg atcacggcag gctgccctgg aagaccttgt tcggaccggc
     3841 catcgccttg tgcgaacagg gctttgccgt ctcgccgcgc ctgcatgcct tgctggaaga
     3901 agagcgcgtc gagctgctga agcagccggc cgccgcgcat catttcttcg acgccgacgg
     3961 ccgggcctgg cccgtgggcc atgtgctgaa gacccccgag ctggccgccg tgctgcatcg
     4021 cgtggccgac gaagggcccg atgccttcta ccacggccag atcgcgcacg acatcgtggc
     4081 ggccgtgcgc gggcacgcgc agccgggcga cctgagcgag gccgacctgg ccggctaccg
     4141 ggccgtggtg cgcgcgccgg tgtgcggccg ctaccggggc tacctgctgt gcgggccgcc
     4201 gccgccttcc agcgggccga tgaccatcct gcagatattg ggcgagctcg agcaatatcc
     4261 catggggcgc atgcagcccg agtcgctgca ggccgtgcat tactttgccg aggcgtggag
     4321 cctggcctac gccgaccgcg atttctacga agccgatccg gccttcgtga acgtgccggt
     4381 cgatgcgctg atcgacccgc gctatctgcg cgcgcgcggc gcgctgatcc ggcccgaccg
     4441 cagcatgggc gaggcccggc ccggcgaccc ggcggggatg ctgcaggcgc gcgggaagga
     4501 cgacgctttc gaggtgccgt ccacttcgca cctggtggcg gtggaccgcg acggcaatgt
     4561 ggtgtcgatg acgaccacga tcgaatcggc cttcggcagc aagatcatgg tgcacggctt
     4621 cctgctgaac aacgagatga cggacttctc gtcctcctac caggatgccc agggccgcct
     4681 ggtggccaac cgcgtcgagc cgggcaagcg gccgcgcagc gcgatgtcgc ccatgatcgt
     4741 gttccgcatg ggcaagccct acctggccac gggttcgccg ggcggcagca ccatcatcaa
     4801 ctatgtggcc aagaccctga tggcggtgct ggactggcgc ctcgacatcc agcaggcggt
     4861 agcgctgccg aacctgggca gccgcaacca cggtgtcgag ctcgaaaagg gcacggccct
     4921 ggtgaagctg gaaccggcct tgcgcgccat ggggcacgag atcgtggaaa ccgattttcc
     4981 cagcggcctg cagggcatcg tgatcgggcc ggacgggctc accggcgggg ccgatccgcg
     5041 ccgcgagggc gtggcgctgg gcgattgatg ccttgcggcg gggcggccgg ccgccgcccg
     5101 ccgcctaatc cagatggatg ccggccgtcc tgatgacttc gcccagccgg tctttttcgc
     5161 gcttgatgta ggcgccgaat tcctccggcg tgccgccggc cggctgcgcg ccgtcgccgg
     5221 ccaggctgtc gtgcacctgg ggcgcctgca gcgcctggtt cagggccttg ttgagggtca
     5281 gcacgatggg gcgcggcgtg ccggcgggaa ccaggatgcc gtaccaggaa ctgctttcat
     5341 agccggggac cccgctctcg gcgatggtcg gcacgccggg cagcacgggc aggcgctccg
     5401 tgctggtcac gcccagagcc accagcgaac cgtccttgat gtgcgggacg atggccacca
     5461 cgctgctgaa catcagcggc accgtgccgc tgatcaggtc ggtcagcgcg gggctcaggc
     5521 ccttgtaggg cacgtgcacc atgttcacgc cggccatggc ggcgaacatc gcggcggcca
     5581 ggtgggagga ggagccggtg ccgttggagg cgtagtccag ctggccgggt tctttcctgg
     5641 ccagggcgat cagttccgcg acggtgcgca catgcaggcg cgggtgcgcg accaggacca
     5701 tgggcgcgcg cgccagcagc gccacgggct cgaagtcctt gatggggtcg tagggcaggt
     5761 ccgtgtgcag gttggggttg atcgcatggc tgcccacacc gcccaggaac agggtgtagc
     5821 catcgggcgc ggcgcgggcc gcggcctcgg cgcccagggt gccgccggcg ccggcccggt
     5881 tgtcgacgat gacggcctgg cccaggagct ccgacagcac gcggccgacc gtgcgcgcca
     5941 cgttgtcgtt gccgccgccc ggcgcaaaag gcacgatcag gcggatggga tggtcggggt
     6001 aggcggcagc cagcgcggcc ggcccgccgg ccgccagggc caggcagccc gccaggacgg
     6061 cgagcaaacg ccggaaggcg gtgcgcggcc gggccggccg ctcgattgcg aaagaaggca
     6121 tgtcgtctcc tgtagggtcc gatggcagac cgggctcttt ttatcggata ccatggtata
     6181 ccaatttaag ggcaggagag acatgccggc gcgccggcgg gttttagcgg aacaccaccg
     6241 tcttgctgcc gttctgcagg atgcggtgtt cgacgtgcca gcgcacggcg cgcgacagca
     6301 ccagcgattc cacgtcgctg cccacctgcg tgagctgctg ggcgctcatg ctgtggtcga
     6361 cccgctcgat gtcctgctcg atgatggggc cttcgtccag gtcggccgtg acgtagtgcg
     6421 cggtggcgcc gatgatcttc acgccgcggg tatgcgcctg gtggtagggg cgcgcgccct
     6481 tgaagctggg caggaaactg tggtggatgt tgatggcccg gccttccagc gcgcggcaca
     6541 tgccggtgga caggatctgc atgtagcggg ccagcaccac caggtcgatg cgttcgcgct
     6601 cgaccagcgc cagcacctgc tgttcctgtt cggctttggt gtcgggcgtg acgggcagat
     6661 agtgatacgg aatgccatag gacgccgcca ggccggcata gtcgttgtgg ttggagacga
     6721 cggccgcgat ctcggcgtgc agctggccgc tgcttgcgcg aaacagcagg tcgttcaggc
     6781 aatggccttg cctgctcacc aggatgagca ggcgcggctt ggtgcgcgca tcgtggatgt
     6841 gccagtccat ggcatagccg gcggccaggg cgccgaactt cacgcgcagg ccgtcctcgc
     6901 ccgccgcggc aggcaagtcg aagtgcacgc gcaggaagaa gcggccggtc tcgtcgtcgc
     6961 cgaactgctg cgagtccagg atgttgcagc ccaggtcgaa cagcaggccg cttacgcggt
     7021 agacgatgcc cgtgcggtcg gggcaggaaa gcgtcaggat gaaatcgttg tgcggcatgg
     7081 aagaatgaaa ttcagggaga caggcgccgc aggacttcct cggctacggc caggctggcc
     7141 gtcaggcccg gcgactcgat gccgaacaga ttgaccaggc cgggtacgcc gtgcgccgcc
     7201 gggccggcga tatcgaagtc ggcggccggc tcgcgcgggc ccgagatctt ggggcggatg
     7261 ccagtgtagc ccggcgccag ggcgccgtcg ggcaggcccg gccagtagcg ccgcacggcc
     7321 gcgtagaaga cgtcggcgcg acgcgggtcg agcgtgtagt ctgcgcggtc tgtccattcc
     7381 gtgtcggggc cgaacttggc ctggccgccc aggtccagcg tcaggtgcac gcccaggccg
     7441 gcgtgggccg gaatgggata gatcaggcgc gagaagggcg cccgaccgga caaggtgaag
     7501 tagctgccct tgcacaggta ttcggcgggg atgctggcgg ccggcaggcc cccgatgcgc
     7561 cgcgccaggc cgggagcatg caggcccgcg gcgttgacca gcacattgca ggacagcgcc
     7621 atggcctcgc cgccgccgaa ccgtgcctcg aatccaccct cggggcgcgc acgggccgat
     7681 tccaggggcg tgtggaagac gcattgcgcg ccggcgttct cggcatcgcc ctggtaggcc
     7741 agcatcagcg catggctgtc gacgatgccg gtcgagggcg atgccagcgc cgccgtgcaa
     7801 tgcagggcgg gttcgagcgc ggcggcctgc gggccggtga tcttttgcag gtcgtccacg
     7861 ccgttggcgc gcgcgcgggc ggcaatgccg tcgagcaggc cggcttcttc gacggacgtg
     7921 gccaccagca gcttgcccag ccgccggtgc ggcacgccgt gctcggcgca gtaggcatag
     7981 agcagttgcc tgccgcgcac gcacaggcgc gccttgaggc tgccggcggg atagtagatg
     8041 ccggcatgga tgacttcgga gttgcgcgag ctggtgccgg tgccgatggc ttcggccgct
     8101 tcggcgacca ggacttcgcg gccctgcagg gccagggcgc gcgccacggc caggccgacc
     8161 accccggcgc cgatgaccag gcagtcgacg tcggcgctca tatcgccccc ttggcgctgc
     8221 gcgccgtccc cccagggggg aacgagcgct tgaggcggct cggcgcgctc atgattccaa
     8281 gggctgttgc cggggcgtga gatcgaagcc gccggcatgg aagaccaacg gcggcgcatc
     8341 gctgtagccg cagtgctcga cttcgcccac catgatgtag tggtcgcctt cctgatagcg
     8401 gctgcggttg cggcattcga accaggcggc gctttcgccg gccagcatga gcgtgccgct
     8461 gggcgcgcgg gccaatgcca ggccggtgaa gcgttcggcc gccgtgcccg tggcgaagcg
     8521 gcgcgccagg gcgatctgct cggccgacag cacgtggatc acgtaacgct cgcattgctg
     8581 catggccgcc agcgacgacg aggacatgcc caggctccac agcaccagcg gcgggttcag
     8641 cgagaccgag ttgaaggagc tgacggtcag gcccaccggc gagccgtcgg cggcctgggt
     8701 cgtgatgagc gtgacgccgg tggcgaaatg gccgagggtg gtgcgaaagt ggctggcgtc
     8761 gaagctgggg gagaaggaca tggtcttgaa gacgtcaggg agagagccgg tcgatcttcc
     8821 agccctgccc gtcgggcagg tagcgcagcc ggtcgtgcag gcgggacgga cggccctgcc
     8881 agaattcgaa ggccgtgggc accaggcggt agccgcccca gtgcggcggc cgcggcggct
     8941 ggtcgccata gcgctcgcgg aatgcgcgtt cgcgggcttc gagcgcgtcg cgcgtggtgg
     9001 gccggctttg ctcggaggcc caggcgccga tgcgcgaacc gggcgggcgg ctgtggaaat
     9061 agcggtcgga ctcttcggcg gacactttct gcacccggcc ttcgatgcgc acctggcgct
     9121 cgagcggctg ccagaagaac agcagcgccg cgcgcgggtc ggcggcgatg gcctcgccct
     9181 tgcgcgattc gtagttggtg aagaaggtga agccgcgttc gtcgacgttc ttgaccagca
     9241 cgatgcgggc cgacggctgg ccgtcggcat cgaccgtggc cagggtcatg gcattgggtt
     9301 cgggcgcctg ggcggccatg gcctcgtcga accagcgttg gaactgctcg atgggcgatg
     9361 cggccgcctg gctttcgagc aacacgtctt tttcgtagct ctgtcgcagg tcggagaggg
     9421 gcatggatgg gactcgcatg gggccgtgcc ggcccggaat gactgcggcc agtgtaccgc
     9481 cgatgattcg cgttccggac gtatgagctt attcgtccgg caagggttcg ccggggcgtt
     9541 cgaggtggca ggcgatggtc tcgagacgcg cgtcgcagat gcccgaggcg cgcagggcgc
     9601 gcacggtttc catgacgtag tcggtgcagg ggccgtagcg cccggccgcg cggcgcacga
     9661 tggcgaccag ttcggcgtcg ggcagggtgc ggatgtaggc cggattgtcg cggttcatca
     9721 cgaagaccag cgccttgacc atgccctggc cggtctcgca attgagccag cgcggcaggt
     9781 aggcgcccga agacatctcg cgttcccaga gcttgggaaa gtgcgtcggc acctcggcgc
     9841 cggtcagccg gaacaccacg ccgcggcagg agccgccgcg gtccaggccg aacaccaggc
     9901 cggggcattc cggcgtgccg cggttgacgc gcgaccacag gcacagggcg cggtgatggc
     9961 cgcgcagggt ggccagccgg cgctcgacgt aggtgaactc ggggcgccag atcagcgagc
    10021 cgtagccgta gacccagaca tcctcgccgg agcgccagtg cctgagcgag gcttccatgg
    10081 aagcctgccg ctgttcgggc gtccagcgtt cgaagggagg caggggggac gaggcggggg
    10141 agggggcggg cgtgctcacg acggtgttgc ggaagtcagg agggttgcgg gttggggcgg
    10201 acggcggttg gcccagccgc ccagggccat gcaggcggca taggcccaga acagcggcat
    10261 gatgccaatt accgcaccga gcgcgccgaa ggtcaacggc aggcagacct gcgaggtgct
    10321 gatgaaggcc agccgcaggc ccgaggcctc ggcggcccgt ccgggcggcg catggtggtg
    10381 cagcagggac agcatgctgg gctggcagga gcccagcgcc aggcccagca cgaaggacag
    10441 gccggcgagc gtggtcgact cggaaaagaa ggggtagatg gaaaaatccg tcgcggtggt
    10501 gatgatggtg gcgcgcacca ggttccaggg ctgtacgtgc ttctggatca tcggcaggaa
    10561 caggcggatg acgaaggtgg ccgccgagaa ggcggccagg atcacgccga tggtggtggc
    10621 cgacaggccg atctgcgtgc cgtagatggg cacgacgaac aggtgcgtgt cccaggcgct
    10681 ggacagcagg gtgttgacca gcagcacctg ccgcagggtc ggcgtggcca gcagttcgag
    10741 catcttgccc tgcttgcctg cgcggctgcg caggtcgccg gacgacaggg cggcgttggt
    10801 gtcgcgcagg ggccggcgca gcaggtacag gcccgcggcc gaggccagcg gcgccagggc
    10861 cagcaggccg aaggccatgc gcgagcccag gtggtcgagc gacagcccgg cgatcagcgg
    10921 accggaaaag ccggacaccg actgggacag ggccagccag gaaaaattgc gcagccgccg
    10981 ggtgggatcg gccgtgccga gcaggtcctg gacggccatc tggttgagca tgaagccgat
    11041 gctgatgctg aaggcggccg cgagcagggc ggaacgggtc tggaacagaa agggcagcag
    11101 ggctcccagg cacaggaggg tgctgccgat gaccaggggg cgccgggcgc ccacgtggtc
    11161 gatccaacgg ccggcgtgca ccgacagcag catgggcatg acggcgaaga tagccaccaa
    11221 tgtgcctact tcgagggtgg atgagcccag tttgagcgca gttagcgagg tggtgatgcg
    11281 cccgccggcc agggccacat ggttgagcat ggccatcagg cacaggcacc ccgccctcgg
    11341 gaactcgggg gcggacagcg ggtgtggcgg aggaagagag gggttggagg taggcacggc
    11401 ggtatcttca tttattttgt gcctatctcc ctggtctgtc gatgcgccgg cgggcctgaa
    11461 ttgacaacct gtgtcatgtc cgcaacgacg tgggaaaata ccgatcacca tgaatgaaat
    11521 gcctgtttcc agcgagatcg acgccgatcg ccgtttcggc ggcctggccc gcctgtacgg
    11581 ccccgggtcg cccgagcgac tgcgcgccgc gcacgtggcc gtggccggcc tgggcggcgt
    11641 ggggtcgtgg gcggccgagg cgctggcgcg ctgcggcgtc ggcgccctga cgctggtcga
    11701 cctggaccat atcgccgaat ccaacgtcaa ccgccagatc cacgcgctca cgcccaccct
    11761 gggccaggcc aagatcgagg ccatggcgca gcgcatccgc gacatcaatc ccgattgcgc
    11821 ggtgacgcgg gtcgacgact tcgtcgaacc gggcaatgtg gccgaggtgc tggcgggcga
    11881 ctatgccgcc ctcatcgatt gcaccgacca ggccgccgcc aagatcgcca tgatcctgca
    11941 tgcccgcgcc cgcggcgtgc ccttcgtgct gtgcggcggg gcgggcggca agacggaccc
    12001 gctggccttg cgcgcgggag acctgtccga ggcggtgaac gatgccctgc tggccaagct
    12061 gcgcaacacc ttgcgccgcg agcacggttt tccgcgcggc tcggaccaga acggcaaggc
    12121 cttgaagcgg gtgccgaaga tgggggtgcg cgcgctgtgg ttcgaccagc cggccatcct
    12181 gccggatgcg tgggcccagt ccggttccga tgagcggagc gcgccgcagg ggttgtcgtg
    12241 cgcgggttac gggtcggtgg tgatggtgac cgccgcgatg ggcatggcgg cggccaatga
    12301 ggcgttgcgg cggatactct gaggttcgtg cgggagttct tggtttggtt gggtggtcgg
    12361 catgttcttg agccgcctag ccgccgtggg cgtaaagcgc tccggcttcg atgaatcggg
    12421 ctcgggcgca accaggcgcc ctcggcccgc tgcgcgggct gccccgattc ataggcgcct
    12481 gcgcgcttta cgcccacggc ggctaggctc agattacggt tcttccagca gccattcatg
    12541 tagtctcgcg gacggcgggc aggccctggg ggcgcctctg aggcggggca gcccgctcag
    12601 cgggccgagg gcgcctgcat gcgcccgagc ccgaatcagc gaagccccca gggcccacct
    12661 gccaaccgca ggacgggtca agaactcgac ggcaagacag acgaaaactc agccctcgac
    12721 gcccacgaag acgtggccat cctcgatcct gacggggtag accgccacgc cctcggtcaa
    12781 tggatcgcac agggccttgc cggtgcgcac gtcgaagcgg ccctggtgca gcgggcactc
    12841 gatctcgtgg cccatcagga agccgtcgca caggctcgcg gcgccgtgcg tgcacatgcg
    12901 gtcggtggcg tagatctcgc cctccacctt gtaaagcgcg atttctcgtt cgccggcctc
    12961 ggccggcgtg acgtcgtcgt ccgtcacgtc gtcctgggcc atcgtcttga tccattgcat
    13021 ggtgccgctt cctcaaatgg ggtggatgag cgaattgggg atgagctcgc tgtcgaacac
    13081 gcatagccgc gactcgaact tcagcccgtc ggcggtatcg cggatcacgt cgaggtagcg
    13141 cccgacattg aataccatcg aaggttggcc cggtttcgtg cggaacaccg cgtaattggc
    13201 ctcggcctcg acgcggccgt cccgcgcgga caggatgcgc ggcgcgccca ccacgtgccg
    13261 ctggtagtag gggtcgtgga agatggtccc cgtggtgccg tagatacggt ccttgagcat
    13321 gccgcgggat tcgaacgaga gcgtggccag cgggtagccg cggtcgtagt tctcgcgcgg
    13381 gatcaccttg taggtgcagt cctccaggaa aaacccgggc cacttgtccc attcggcggc
    13441 atccagcgcg gcggcgtagt cgccgtagag gcgggcgatg cggtagtaca gatcgaaatc
    13501 catcgcctca gccctccatc acgccgcgcc agtatcggta catgccccgg atcagcgtct
    13561 cggtgaccat gtggtcggtg ttctcgaccg tcttgccgcc cagctcggcg acgcagcgat
    13621 gccagggctt ctgctcgaag ccttgctgcg agaactcgat gacctcgccg tcgtcggcgg
    13681 acacgaagcc ggccgggccg aacagattgg cctggcgcag gcggcgctgc gtcatctcgg
    13741 gcgtgtcctc ggcaaagccg aagtgcgtcc agacgaagtc gaaggcgtcg tgccccaccg
    13801 gctggatgtg ccgggtggac agcgaattga cctgctgctg gatgatgacg ctggggaaca
    13861 gcgtcatgag caccgccgtc gggccctgcc accagggttc ggtgacgatg tccaggaagc
    13921 gcgggtcgtt gagctgcatc tgttccttga agctggtcac gccgctggtg acggcgccct
    13981 tgccgccctg gccgcgcgtc gagatcatgg cggcgtggcg gccgtggctg tccatcctga
    14041 gttcggacct gttgtcggcg cgccagagcc cgaaggtgac gaaccaggta tgcagcaggc
    14101 cgggatggta ggggtccttg atgttttcct gcatcagctt ccagttgccc ggaatgcgct
    14161 ggcggctgta gccgtggatg accagttcgc ggccgtcgaa gacgcggtcg aagtagggca
    14221 ggatggcggg ccccaggtag tcttccagcg gttcgacgcc gtggtcgaac gaggcgaaca
    14281 ccacgccgtt gcgcacggcc accttgagct tggtcaggcc gtggtcttcg gtcttgaagt
    14341 cgggcggcat gccgccgttg accttgccgt cctgcttgac gccgcggcga aagggcacgc
    14401 cgatcaggtt gccccgcagg tcgtaggtcc actggtggta ggggcagacc agatcggtgc
    14461 ggttgccgta gggctcgcgg cagaactgca tgccgcggtg cgcgcagacg ttctcgacga
    14521 cgcggatttc gccatccttg tcgcgcgcca ggatgaccga gcgttcgccc acgaccgtgc
    14581 gcttgaagtc accgggcctg gggatctcgg ccgtcaggcc cacgtagcac cagtgggcgc
    14641 ggtagaagag gcgttcgagt tcgcgctggt gcagcgcttc gctggtgtag acgccgaagg
    14701 gaatgcggtt cgaaccgctt tccggccagg cgggcagggg ggtgtcgggc atgcttgtct
    14761 ccatcttgtc gtggataggc cgcggctgtg ccgggccctg ccgcccgccg gcgggggcgg
    14821 gacagtggcg gcatcatcgg gcaaacgtca aaataaataa atagaattta aagaatagaa
    14881 aatatcactg atgtgaatat tgtcggaggg actccgtgaa tcttcatgaa ctggatttga
    14941 acctgctggt cgtcttcgat gccctgcacc ggcatggccg ggtatcgctg gccgcgcaga
    15001 gcctgggcgt ctcccagccg ggcgtgagca atgccttgcg gcggctgcgc aagctgctcg
    15061 gcgacgagct gttcctgcgc acttcgggcg gcatggtgcc cacgccttat gcgcagtcgc
    15121 tggcccagcc catttccgac gcgctggcct ccctgcacgc gacgctcaat gcccatgcgt
    15181 cgttcgaccc gggccgcagc gaacgcaact tcgtcatcgg cgtgaacgac gtgggcgaac
    15241 tgtatttcct gccgcgcctg atgcgcgcgc tgggccgcga tgcgcccggc gtgacggtgc
    15301 gggccgagct gacgggcggc gtggatgtga aggaagaaat ggagcgcggc cggatcgacc
    15361 tggtgctggg tttcctgccg ggcctgaaaa gcggcttttt ccagcgccgc ctgttccgcc
    15421 agccctatgt gtgcattttc cggcaggacc acccgctggc gggcacgggc gtcacgctca
    15481 agcagttcca ggcggcggag cacgcggccg tggtgtcggg cggcacgggg catggcgtgg
    15541 tggacgaaat catcaatcgt ttcggcgtgc ggcgccgcgt caagctcacc gtgccgcatt
    15601 tcatggcgct gggcccgctg ctgcaggcca ccagcctgat cgccgtggtg ccgcggcgtt
    15661 ttgccgatgc ggcctgcgcg ccgttcgggc tggccactgc ggcctgcccg gtgaaaatcc
    15721 ccgaatcggt gatcaatgtg ttctggcatg cccgcaacca ccgggaggcg gccaaccagt
    15781 ggttgcgcaa gcgggtggtc gaggaatttg ccgattgacc gggcatcggg aataaactac
    15841 cggcgtccgc tgcccaggat gggcggcgcc acgttaaggg agactcattc atgcaaagaa
    15901 aactcatggc cgcgctggcg ctgtcggccg ctgccgccta ttcgttcagt gcccaggccg
    15961 cggatacctg gcccagcaag cccctcacca tcgtcgtgcc cttcgcgccg ggcggcgcca
    16021 acgacctgat cgcgcgcctg tacgccaagc agatcggcgt cgagctcaag gccgatgtca
    16081 tcgtcgagaa ccgcccgggc gccggcgccg tggtgggcac ggcccacgtg gcgcgcagcc
    16141 agcccgacgg ctacaccttc ctggtggcgt ccaacggcac ggtcaccaac accctgatcc
    16201 gctccgacca gccgttcaag gacgacgaga tcgagccggt ggccatgctg gccatcgtgc
    16261 cctcgatcat cgtcaccaac ccggccaatc ccgccaagga cctgaaggaa ctggtcgaga
    16321 acgccaagaa gaattccagc cgtcccgtga ctttctccac cgcgggcatg ggcagcacgc
    16381 ccgatttcgt gggcgccatg gtcaagctgg aaaccggcgt caacatgcag acgattccct
    16441 acaagagcgg cgccgagaac gtggccgcgg tggtcggcgg ccagaccgac atgacttccg
    16501 aagccgacat ggtcaccctg ccgctggtca aggcgggcaa gctcaaggcc ctggccacca
    16561 cctgggatca tctggccgtc gcgcccgata tcaagactac ggcggagctg ggcttcccca
    16621 atatccgcat cggccactgg gaaggcctgt atgtgcgcgc cggcacgccg gccgacatca
    16681 tggacaaggt gaacgccgcg gtgctgcgcg cctcgaagtc gaaggagatc ctggacgtgc
    16741 tcaatgccta cagcgtcgtc cccggcacga tgacccgtcc cgagttcgtc aagttcacgc
    16801 aggacgagcg cgtccgcctg ggcaaggtgg tgaagggcgc caatatgcaa cccgattgat
    16861 tgcgggttgg caagtcggat tgaacttcgt ttgagttctt gagccgttct tcggtcggcg
    16921 gcgtcaccct gggggcttcg ctgtgccggg ctcgggcgca accaggcgcc ctcggtcccc
    16981 ttcggggact gccccgtccc agaggcgccc ccagggtgac gccgccgacc gaagaactac
    17041 ggcaaggtca tacgacggct atccacggtc gtcgtatggc gctaatgcag ttcctcatct
    17101 ggcgggtatg ccccaggggc gcctctgtga cggggcagcc cgcgcagcgg gccgagggcg
    17161 cctggttgcg cccgagcccg gcacagcgaa gcccctgggg catacccgcc agatgaggaa
    17221 cgggtcaaga acgcaatcgc catgggccga gaacccagcc gaacgcctta agacctggcc
    17281 gacaggaact cgcgcaggat ccgccccgtg tgcgacttgt ccgcacgttt catcacggct
    17341 tccggcgcgc cttgcgccac cagccgcccg ccgccatcgc cgccctccgg ccccatgtcg
    17401 atgagccagt cggcctcggc gatcacatcc aggttgtgct cgatgaccac cacggtgttg
    17461 cccgcatcca ccaggcggtg cagcacgtgg atgagctttt ccacgtcggc catggacagg
    17521 cccaccgtgg gttcgtcgag cacgtagagc gtgtgcgagg tcgcgcgtcc ggcccggccg
    17581 gtcttgaaca ccccttcggc ggtgcccagc ctggccttgg ccagctcggt caccagcttg
    17641 atgcgctgcg cctcgccgcc cgagagcgtg ggcgaaggct ggcccagggt gaggtagccc
    17701 aggcccacgt cctgcatcag ctgcaagggg cgctggatct tgggatgcgc ggcgaagtag
    17761 ccgatggcgt cgtccacttc catgccgagc acgtcgccgg cgctcttctc gcgcagcttc
    17821 acgccgagcg tgtcgctgtt gaagcgcgcg ccgttgcagc cgtcgcaggg cactttcaca
    17881 tccggcagga agctcatctc gatggtgcgc atgccctggc cttcgcagac cgggcagcgg
    17941 ccttcgccgg tgttgaagga aaagcgcgcc gcggtccagc cgcgcatgcg ggcttcgcgc
    18001 gcgtcggcgt agagcttgcg cacgtcgtcc cagaagccca cgtaggtggc ggggcaggag
    18061 cgcggggtct tgccgatggg ggtctggtcg acctccagca cgcggtcgat caggtcccag
    18121 ccttcgatgc gttcgcagcc gctccagccc ggggccttgc cttgcgacac ggcatgggtg
    18181 aggttgtcga gcagcacctc gcgcgccagg gtggacttgc ccgagcccga gacgccggtg
    18241 acgacggtca ggcggccgat ggggatgtcg gcgtcgacct tgcgcaggtt gtgcaggcgc
    18301 gcgccgcgca cctggatcat gggcgtgtcg cttgccaccg cgcgccggcc gttgagggga
    18361 tgggccagcg gatgggccag gtagcggccg gtgatggact cgggcgcgtc gatgaggtcc
    18421 tgctccgtgc cctgggccac cacgcggccg ccgcgcacgc cggcgccggg gccgatgtcg
    18481 atgaggtgcg aggcgcgccg gatggtgtcc tcgtcgtgtt ccacgacgac cagggtgttg
    18541 ccattgccct ccagccgcgc cagcgcgtcg agcaggatgc ggttgtcgcg cgggtgcagg
    18601 ccgatggtgg gttcgtccag cacgtagcac acgccctgca ggttggagcc cagctgcgcg
    18661 gccaggcgga tgcgctgcgc ctcgccgccg gacagcgtgg gcgcggcgcg gtccagcgcc
    18721 aggtagtcca ggcccacttc ctgcatgaag gtcaggcggc cgcggatctc ggtgaggatg
    18781 tcgcgcgcga tctcgccttc gcggccgcgc gtcaccaggc cggtgaagaa ggtgtgcgcg
    18841 tcgctcacgg gcagcgcggc cagttgcgcg atggaccggt cgcgccatcg cacggccagg
    18901 gcggtgcggt tcaggcgctg gccgtggcag acgccgcaga ccttggcctc gccttcgtac
    18961 caggcgttcc aggcggtttc ctcgccggtc tgttctgcgt cgaagccttg cagctgcagg
    19021 ccggtgccga agcaggccgt gcaccagccg tgcttggagt tgtacgaaaa gaggcgcggg
    19081 tcgggctcgg ggaagctctt gccgcaggtg gggcaggccc gcttggtgga gaagtgctgc
    19141 tgttgcagct cggaggacag cgactcgtgc agcgtgtcca ccggccatac cgccgacatc
    19201 acgccctggc cgttttccag ggcgctcttg acggcggcgc gcaggtcggc ctcgttggcc
    19261 gggtcgacca ccacgtcggc tacgggcagc tcgatggtgt gttccttgta gcggtccagg
    19321 cgcggccagg gcgagacggg gatgaactcg ccgtcgacgc gcaggtgggt gtggcccttg
    19381 gcgccggccc acttggccag gtcggtgtag tagcccttgc gcgcggtgac cagcggcgcc
    19441 agcaggccga tgtgcacgcc cttgtgatcg cgcagcaggc gcgccacgat ctggtcggac
    19501 gtctgcggct cgaccggcac gtggtcgtcg gggcagtact gcgtgcccag cttcacgtac
    19561 aagaggcgca ggaagtggtg gatctcggtc atggtcgcca cggtcgactt gcgcccgccg
    19621 cggctggtgc gctgctcgat ggccacggtg ggcgggatgc cgaagatggc gtccacgtcg
    19681 ggcttgccgg ccggctgcac gatggcgcgc gcgtaggcgt tgagcgattc caggtagcgc
    19741 cgctggccct cgttgaagag gatgtcgaaa gccagggtgg atttgcccga gcctgaaacg
    19801 ccggtgatga ccgtgaactt gtcgcgcggg atctccacgc tgacgttctt caggttgtgc
    19861 tcgcgcgcgt tgaggatctc gatggccggc gcgcccttgc gcttgcggcg catgagcgat
    19921 tgcaggggcg tgccgctgcc cgtgccgttt tcggcgccat aggcgacggt tggctcggcc
    19981 agggcgggag ccggggcgct ggcggcctcg accggcagga tcgagactgc atagtcgcgc
    20041 agggcctggc ccgtgtacga acgcggattg tccatcaggt cttgcggcgt gcccacgccc
    20101 accacttcgc cgccggcatc gccgccgtcc ggacccaggt cgatgagcca gtcggcggcg
    20161 cggatcacgt ccaggttgtg ttcgatgacg agcagggtgt ggcccgccgc cagcagcttg
    20221 cggaaggcgc gcatcaggcg cgccacatcg tcgaagtgca ggccggtggt gggctcgtcg
    20281 aagaggaaca ggctgccttt cttggccacg cgggcggtgg agatgccgct gcgcgcggcc
    20341 tcggccagat ggccggcgag cttcaggcgc tgcgcctcgc cgcccgacag cgtgggcacg
    20401 ggctggccca ggcgcacgta ttccaggccc acgtcggcca gcggcgccag gcccgtctgc
    20461 acgtcgcgca ggcccttgaa gaagtccagg gcctcgctca ccgtcatgtc cagcacctgg
    20521 tcgatggagg cgctgcgtcc caggtgctcg acgcggactt ccagcacttc ggggcggaag
    20581 cgcttgccgt cgcagtcggg gcagcgcagg tacacgtcgg agaggaactg catctcgacg
    20641 tgctcgaaac ccgtgccgcc gcaggtgggg cagcggccgt cgccgctgtt gaagctgaag
    20701 gtgccgggca tatagccgcg ctcgcgcgcc agcggggcct gggcgtagag cttgcggatg
    20761 gcgtcgaagg cgcccacata gctggccggg ttggagcggg ccgtcttgcc gatgggcgtc
    20821 tggtcgacca tgaccacgtc ggccagctgc tcggcgccga gcagcttgtc gtattcgccc
    20881 ggggtctcgg taggcttgcc caggtatttg agcagggcgg gatggagcac gtcctgcacc
    20941 agggtggact tgcccgagcc ggacacgccg gtcacgcaca ccagccggcc cagcggcaat
    21001 tccaccgaga cgttgcgcag gttgtgggcg cgcgcgcctt ccaggatgag gcgcggcgtg
    21061 ctggcggcca ccggcatggg acgcggggcc tccacgcgca gccggccgcc caggtagtcg
    21121 ccggtcagcg tgggcgcgac gcgcagttgt tcaggcgtgc cgtcgaacac gatgttgccg
    21181 ccgcgctcgc ccgggccggg gccgatgtcg atgatgcggt cggccgccac catcacttgg
    21241 ggatcgtgct ccaccaccac cagcgtgttg ccggcgtcgc gcaggcgatg catgacctcg
    21301 accacgcgat gcatgtcgcg cgggtgcagg ccgatggacg gctcgtcgag cacgaacagg
    21361 gtgttgacca gcgaggtgcc cagcgccgtg gtgaggttga tgcgctgcac ctcgccgccc
    21421 gacagggtgc ggctctggcg gtcgagcgtc aggtagccca ggcccacgtc gcacaggaac
    21481 ttgaggcgcg cgcgtgcctc ggtgagcagc aggtccgagg ccgcgtcgag cgcgccctcg
    21541 aagcgcaggc cgtcgaagaa gcgcttcacc cgttcgatgg gcagcagcat catgtcgtgg
    21601 atcgacaggc cggccatggc gccgagctgt tcgcgcgtcc agctggcgct ggcgggcatg
    21661 aagcggcgat agcggccttc gtgggcgcct tctcccggca gcacgtcgtc ggcgtcctcc
    21721 cggctgccca ggcgccacag cagcgcgtcg ggcttgaggc gcgcgccctt gcacaccggg
    21781 cagggggtgt agctgcggta tttcgacagc agcacgcgca cgtgcatctt gtaggccttg
    21841 gtttccagcc attggaagaa gcggtgcacg ccgtaccact gggtcttcca ggcctggctg
    21901 ccgcccttcc agtcgggcgt gccgtcgagc acccaggcgc gctgctccgc gctgagcgac
    21961 ttccagggca cggaaagcgg aatgccggcg cgcggcgcgt atttctcgag ttcggtctgg
    22021 cattccttga acgagggcgt ctgccagggc ttgatcgcgc cttcgagcag ggtcttgttc
    22081 tcgtcgggga tgaccaggcc caggtcgatg ccgatcacgc ggccgaagcc gcggcaggcg
    22141 tcgcaggcgc ccagcggcga gttgaaggaa aaactgctgg gcagcgggtc gctgtattcg
    22201 atgtcgcagt cggcgcagtg caggcggttg ctgaatttcc agacggaagc gtccttgcct
    22261 tcgtcgtcca tcgcgtgaat ggaaagatgg cccgcgccca tgcgcagggc ggtgtccagg
    22321 gcttccatca cgcgctcggg ctcggcgctg gagaagcgga agcggtcctg caccacatgc
    22381 aggatgcgct gcatctcgac agcgggggcc gccgccttct tgcccttgcc cttggtcttg
    22441 ccgccggcct tggcgccgtg cgaggccgcg cccgcacggg gcgcctcttc gctatgcacg
    22501 cgggtgtagc cctgctggtc gaggaagcct ttgacttcct cttcggtgaa attggccggc
    22561 acgcccagcg ggaaggtgac gaccaggcgg gggtcgcccg gcccgtccgc cgccgggccg
    22621 ccccaaggcg gacgagcccc ctcggggggc agcaagcccg cagggcgcag cgtgggggca
    22681 accgtgcttg tgcgttcggc gatggcggcg cggatggtgt cgggcgtgtc gcgccgcacc
    22741 ggccggccgc agccgcggca gtagagcgtg gcgccgcgcg cgtacagcag cttgaggtgg
    22801 tcgttgagct cggtcatggt gccgaccgtg ctgcgcgagc tgcgcaccgg attggtctgg
    22861 tcgatggcga tggccggcag gatgccttcg atgcggtcga cctgcggctt gtccatgcgg
    22921 tcgaggaact ggcgcgcgta gggcgagaag gtctcgacat agcggcgctg gccctcggcg
    22981 tacagggtgt cgaaggccag cgagcttttg cccgagccgg agacgccggt gacgacgatc
    23041 agctcgccgg tggcgagggt cagatccagg ttcttgaggt tgttctggcg ggcgccaacg
    23101 atgcggattt cggagctcat gggggaatcg tagcgggtgc gggacggcgg gagcgatggg
    23161 tgcgtatctg tatatatgaa cagtatctca gattcatggc atgtgcctga cagcccgacg
    23221 cctcgacagg gtcaaagggc ttgtggtgta atgcctacac tatggaaaca atgtccacat
    23281 tatggacaaa ttagaaaacc acagcgctgc aaaaagcctg ggcgcatgag ggtggagacg
    23341 tggtctacac gctggatttc tcgacggtat tcaagtcgta cgacgtattg ctggccggtg
    23401 cctggctcac catcgaattg tcggccgcgg ccatggcgct ggggctgatc gtggcggtgt
    23461 tcggcgcgct ggccaagctg ttcggcccgg gctggctcaa ggcgctggtc gatgcctata
    23521 tcgagatcat ccgcaacacg cctttcctga tccagatctt cctggtgtat tttgggctgc
    23581 cgctggcggg cttgcggctc gagccggtgc cggcggcgct gctggccatg gtggtgaacg
    23641 tgggggccta tgccatcgag atcgtgcggg ccggcatcga cgccatcccg cgcgggcaga
    23701 tcagcgccgg cttcgcgctg ggcctggggc gcctgcaggt gttccgctac gtcatgatcc
    23761 ggccggcgct gcgcaaggtg ttcccggcgc tgaccagcca gttcatctgg ctgatgctgg
    23821 gttccagcgt ggtgtccgcc atctcggccg acgacctgac ccaggtggcc agcaacctgg
    23881 cgtccagcaa tttcaagcac ttcgagatct atatcgtcgt gaccgggatg tacctggtca
    23941 tgacgctgct gttctccggc gtcttcgcgc tgatccgccg ctgggcgttc tcgtatccgg
    24001 agggacgctg agatgacgac ccccacgctc actgcgttcg ctgccccccg aggggcgcgt
    24061 cagcccctag tggcggcccg gcggagctga cgacatgatc cgccccttcg gattcagtga
    24121 attcctcttc atcgtggagg ccgtccgctg gacggtgctg ctgtcgctcg tggcctttgt
    24181 cggcggcgcc gtggtcgggc tgcccgtggc cctggcccgc acggcgccgc cgcgctggct
    24241 gcgcgtctgc gccacggtct acatccagct gctgatgggc acgccgctcc tgatgcagct
    24301 ctttctcatg tacttcggac cgcccgcgct gctgggcgtg gaagtcgatg cctgggtggc
    24361 cgccgccatc gcgctgacct tgaattccgc cgccttcctg gccgagatct ggcgcggctg
    24421 catccaggcc gtcccgcagg gccaggtcca ggccgcccgc gccctgggcc tgcgctatcc
    24481 gagcctcatg cgctgcgtgg tgttgccaca ggccttccgc atttcgctgc cggccacggt
    24541 gggcttcctg gtgcagctgg tcaaggccac gtcgctggcg tccatcatcg gttttgtcga
    24601 agtgacgcgc gccggccaga tcatcaacaa cgccaccttc cgcccggcca tggtgttcgg
    24661 catcgtggcc atcatctatt tcgtgttgtg ctggccgatg tcgcgctacg ccgccgcgct
    24721 ggagcgccgc tacagcgcgg cccaggcccg ctgagcgggc ctgttcaccg gccctggttc
    24781 cactctttgg gctttaccat ttcaggagac aaagcatgct gatgcaaacc gtacgacaac
    24841 tgggccgcgc ggcggccgtg acggccttgc tggccggcag cctggcgctc gccccgtcca
    24901 cgcaggcgca gaccgtggac cagatcaaga gcaagggcac gatcaccgtg ggcgtgatgg
    24961 tggacttccc gcccttcggc ctgctcgaca aggacaacaa gcccgacggc tacgacgagg
    25021 acatcgcccg cctgctggcc aaggacctgg gcgtcaagct ggacttcgtg cccgtcagcg
    25081 gcccgaaccg cgtgccctac ctgctcaccg gcaaggtcga cctgatcatc ggcgcgctgg
    25141 gcatcacccc cgagcgcgcc aagcaggtgc agttctccga tatctacgcc acgaccaagc
    25201 tggccgtcta cggccagaag ggcgtcaagg tcgagtccga gcaggacctg aagaacgtgc
    25261 gcatcggcgt ggcgcgcgcc agcacgcagg acatggccat caccaagcgc gcgccggaag
    25321 gcacgcgcat ccagcgctac gacgacgacg cctcggccgt gcaggccctg ctggccggcc
    25381 aggtgcaggt gatcggcctg accaccacgc tgatccccga gctcgacaag atcgcgccca
    25441 agggcgccta cgagatcaag ctgccgctgg ataccttctc atacggcatc gccatgcgtc
    25501 cgggccagga cgacctgctc aagggggtcg acgccttcct ggccaagatc aagcaggacg
    25561 gcacgctcaa ccagctgcac cgcaagtggc tgggcaccga cttcgtcgaa ccgggcaagt
    25621 cctgatcggc cgcacaggcc ggatgagccg ctgatcatgg tttccaaacc agcgtccgcc
    25681 gtggccggca cggccgcgtt ctcgaagttc atggacgtgc tggagcgggt ggccgaggcc
    25741 gagcagccgc caggcgtggc cgagttggcg cgcgcgagcg gctacccccg gcccaccgtc
    25801 taccgcatcg tgtcggccct gctcgagcat gggctgatca gccagcggcg cgaaggcggc
    25861 gcctacgaac tgggtccccg gctgttgagc ctggccagcc gcagctggtc gcgcttcgac
    25921 ctgcgcgtgg cgctcgagcc cgagctgcgg gcgctgcgcg acgccacggg cgagacggtg
    25981 cacatggccg tgcccagcga gggcgccatg atttatgtgg aaaagatcga aagccccggc
    26041 ccgctgcgca tggtgtcgcg cgtgggcgga cggctgtcgc tgcattccag cgcggtgggc
    26101 aaggcctacc tggcggcctt gcccgagcag gaatgcgagg cgctgctcga caggctgccc
    26161 atgccgggct tcatgcccaa caccatcacc acgcagccag ccatgcgcgc cgagctgcag
    26221 cgcgtgcgcg agcagggcta cgcggtggac aacgaagaaa acgaggcggg catcgtctgc
    26281 tatggcgttg tgctgtgcga ctcgggcggc aatccggtgg cgggcgtcag cgtgagcacg
    26341 ctgctgtttc gccagcagcg cgacccacgc gcggcctacg tcgagccctt gctgcgcctg
    26401 caggccgcgg cggcccggac cctgtatgcg gggcccgtgc aggccggcgc gggcaagatg
    26461 ccatgacgaa ttccaagagc accccatgaa ccagcctgat tgcgccgctt tccgctacga
    26521 cgtggccggc ctgggcgagg ccctgatcga gttcaaccag acccgggcgg gacggcccga
    26581 ttatctgcag ggcttcggcg gcgacacgtg caacgccgtc atcgccgcgg tgcgccaggg
    26641 cgcgcgcggc gcctacctga cgcgggtggg cggcgacgcc ttcgggcagc agatcgtgga
    26701 cctgctgcgc gccgagggcg tcgatgccgc cggcgtggtg gtcgaccccg atacctatac
    26761 cggcatgtat ttcgtgcagc acgggcccga tggccatgtg ttcagctacc tgcgcggcga
    26821 ctcggcggcg gcccgcatgc agccggccat gctggggcat ggcctggtcg agcgggcgcg
    26881 cttcctgcat gtgtcgggca tcagcctggc catcagcgca agcgcctgcg acacggtgtt
    26941 cgccgccatc gcgcgcgcca gggcggccgg gacgcaggtc agcctggatt ccaacctgcg
    27001 cctgcggctc tggccgctgg accgggcccg cgccatcctg cgcgaggcgc tgtgcctggc
    27061 cgacctgttc ctgcccagcc tggacgatat gcgccatctc accggccatg aagacccgca
    27121 ggccacgctg gactggatac gccaggccgg cgcgcgcggc gtggtggtgc tcaagctggg
    27181 caaggacggc gcggtgctgg acgacggcgc cacgcgtacc cgggtgccgg gccatgtagt
    27241 gcgggcgatc gacgccaccg gggcgggaga ctgcttcgcc ggcagcctgc tggcgcggcg
    27301 ctgccagggc gacggctggc tggcggccgt gcgctatgcc aatgcggcgg cggcgctgtc
    27361 cacgctgggc tggggggcgg tggcgcccat tcccacgccg gcgcaagtga aggcggcgct
    27421 ggcggattag cgcgctcctg gtttgtccat ttgccggata ggggtgttca gggtaagatc
    27481 tcagattccc cattttgccg ttggcggttt tgcctgcggt ttcttaacag tttcaggagt
    27541 caaccatggc cgaacgtaag cgtgtgcgcc gcaataccct ggagcgccgt tgcctcggca
    27601 aggcgttcaa gcgtcttttc gtgaaatcgc ccaagggcgt tttcaagatg ctggagaagg
    27661 tcaagcgcgt gtaatttctt cgcgccctga tcaaaggggc cccgcctggt ctgccaggcg
    27721 gggccccttt gttttgtgcg gcataaggcg ggcggcaaga agaactgcgc gggtcgtcaa
    27781 agcgtgacgt tttcattcaa gggcagattg tgggatcggg tttacgcccc taggcttccc
    27841 cttcttgatg atgaccatgt cctcgttcgt tatcgggatg acaatggcgc gttccggatc
    27901 atgtatgtcg ccaagttttc ggacacggtc tatgtattgc atgctttccg gaaaaaacac
    27961 ggaagacatc gaaaaccgat atggaccttg ccgctaagcg ctataagttg attgggagcc
    28021 tggcatgaaa aaacagaccg acccgcgttg ggatgacgaa agcgttacgc gcggcagcgg
    28081 caatgtattc atcgacctgg gcttcgatcc tgccgaggcc gaagtgatgc gcatgcgcgc
    28141 cgaggtcatg atccacatgc gcgataagct gaaggaatat ggctggacgc aagccgaggc
    28201 ggcgcgacgc ctgggcatta cccagcctcg cgtgtcgcgc ctggtcaagg gcaaggtaga
    28261 ggacttcagc ctggatatgc tcctggtgct ggccggacgc gtcggcctgc gcccggagct
    28321 tcgcttgtcc gcctgattct ctgcgcggtc ttacacgcaa atccccaact gcttggcccg
    28381 ccgcatcttg cgcaccgtgg cgtcgaagag cttgatggcg aaggagtcgt cgcggatcag
    28441 gccggcggcc agcgccgagc agcgggtctt gatgatgtcg gcgatgctgg tgttggagcc
    28501 cggcaggcag ccggcgtggt actggatctc ggccgcgcgc tgcaccgtcc acagcagcat
    28561 gaaggtgctg gggatgtctt tctcgcacac cgcgacgccg tggttgcgca gggccagcac
    28621 gtgcttgtcg cccaggctgg ccagcatgcg cgtcttttcg tcggcgaaca gcgtgatgcc
    28681 ctcgaagtcg tggtagccca cgcggccgaa caattgcgcg ccgtagaaat cgtcgtgatg
    28741 gaagccgtcc tgcttgagcg tgatggccga cacgggattg gtgtgggtgt gggcgatgca
    28801 gtgcacgtcg ggccgcgcgc cgtggatggc gctgtgcagg gcgaagccgg ccgggttggc
    28861 ggggtagggc gagggctcga ccagatcccc ttccaggccg accttgagca ggttctcggg
    28921 cgtgacttcg tagtagttca ggccgaaggg gttgaccaga tagtggttgg ccgggccggg
    28981 caggcgggcc gagatgtggt tgaagatcat ctcggtccag cccaggtagt cgaccaggtg
    29041 gtagaagtcg gccagctgca cgcgcagctg ccattcttcg tcggggatgt gggcgggctt
    29101 caggtaggcg gagttgtcgg cgtgcatggc gggctccaga gagaggtcag ggggtgggtc
    29161 agtagcccga gcgccgcgcg tcgcgaaaac gcgtgatcga cagaatcgag cgggtggtgg
    29221 gcatgggaat atcgtagcgc tcggcgagct ccagcaccgc gtcgccgatg gcggccagct
    29281 ccagcggccg gccggcatgg aaatcctgca gcatcgaggt gcgaaagtcg cccagcttgg
    29341 cggcccgctc gaccatggtg gcggggtcgt tggcgagctg ggcgccatag gcctgggcca
    29401 cggccatggt ttcgcggatg acgttttccg ccaggctgcg cagcggctcc tggccgtaaa
    29461 tctgccccag cgtggcgccg cccgcggcga cggaaagcgg gttcgaactg aggttggcgg
    29521 ccaccttgct ccagatgccg tcgcggatgc gggggctggc ctgggtttcg atgccggcgc
    29581 cggtcaatgc ctggcacagt tgctcgacgc gcgggctgag tccgccgccg ggctcgccca
    29641 gcacgagcct gtgcgggctg ttcgacagca ccgtgccggg cttgggcgtc tcggccgtca
    29701 tgtagaccac gcagccgacc agttgcgcca gcggcgtgac ggccagcagc gcgccgtccg
    29761 gatcgacggc cgcgacgggc ttgcccgcgt gcgggccgcc ttcggcatgg aaataccacc
    29821 aggggatgcc gttgacggcg ggcacgatga cggtgtccgg gccgatcatc ggggcgcaca
    29881 gcgcgagcat ctcggccagg tggtgcgtct tggtgcagag aaagatggcg tcctgcgggc
    29941 cgaagtcggc cgtgtcgctg gccggcacgc gcgcgcgcac ctcgccggag aggtcgcgca
    30001 gggtcaggcc gtcggcacgc agcgcggcca gggtggcgcc gcgggccagc acgctgacgt
    30061 cctggcccgc atgggccagg cgcgcggcca gcgctccccc gatggcgcct gctccggcta
    30121 tgcaaatgcg catgtcagct ccgccgggcc gccccaaggg gctgacacgc ccccgcgggg
    30181 ggcagcgaac atggtgagcg tgggggccgt catgccttgc ctcccatgcc caggtagacc
    30241 tcgatcacgc gcgggtccct ggccagttcg gcggccgggc cttccagggt gacggcgccg
    30301 gtttccagca cataggcata gtcggccacc tgcagggcgg cgcgggcgtt ctgttcgacc
    30361 agcaggatgg acacgcccag cttgcgcagc tcggacacga tgcgcagcac ctcgcgcacg
    30421 atgcgcgggg ccaggcccag gctgggttcg tcgagcatca gcagcttggg cttggccatc
    30481 agcgcccggc ccacggccag catctggcgc tcgccgcccg agagggtgcc ggccagctgc
    30541 gcgcggcgct cctgcaggcg cgggaagaga tcgtagacct cgcgcagcga gatgccctgg
    30601 tcgcggtcgc ccatgcggta gcggtgaaag ccgcccagca cgagattgtc ttccacggtc
    30661 atctcggaaa aaagctcgcg cttctcggga accaggccca tgccggcggc gaccatttcc
    30721 tcgatctcgg cgtgttcgcg ggtctggccg ccgaaagtga tggcgccgcg cgagggcagc
    30781 acgcccatga tggccgacag cagcgtggtc ttgcccgcgc cgttggggcc gatgacggtg
    30841 acgatctggc cttcggccac gtcgatgttc acgccggcga cggcggtgac cttgccgtag
    30901 gcgacctgca ggtcgcggac ttccagtagc ttcttactca ttctcgtcca tcccgcccag
    30961 ataggcctcc agcacggccg gattgttctg gatgtcggcc ggcaggcctt cggcgatttg
    31021 ctcgccgaag tccatcacca cgatgcgatc gaccaggttc atgacgaagt ccatgtcgtg
    31081 ctcgaccagc agcacggcca tgccctcgga acgcaggcgc gacagcagtt gggccagctc
    31141 ctgcttttcc tggtggcgca ggccggcggc gggttcgtcg agcagcagca ggcagggatc
    31201 ggcggccagg gcgcgcgcga tctcgaggat gcgctgctgg cccagcgcga gcgaccccgc
    31261 gggcgtatgc atgaactggc ccaggcccac gcgctcgacc tggcgggccg cttcggccag
    31321 caggcgggct tcttcagcgc ggtcgcggcg ccaggccgag cgcaggatgc cgctcgtgcc
    31381 gcgcaggtgg gcgccgatgg ccacgttctc gagcacgctc atgcggccca gcaggcgcac
    31441 gtgctggaag gtgcgcgaca ggcccagctt ggcgatggcg cgcgcgccga tgccggtgac
    31501 tgcctggccg cgcagcgtga cggtgcccga ggtgggcgtg tccacgccgg agatctggtt
    31561 gaacatggtg ctcttgccgg cgccgttggg gccgatcagc gccaggatct cgccggcgtg
    31621 gatgtccagc gtcatcttgt tgttggccac caggccgccg aagcggcggg tgatctcgcg
    31681 cgcctgcagc accacctcgc cgcgcggcgg catgggcctg cggggcagcg cctcggcctt
    31741 catgtcgacc tggcgctgcg cggcgcggat cggcagccgg tgcgacaggg cggcccagag
    31801 gccggtgcgg gcgcggtgca gcagcagcac catgccgaag ccgaacacga tggtctcgaa
    31861 gttgccggtg tggcccagca ggcgcggcag caggtcctgc agccactcct tgaggatggt
    31921 gaagatgccg gcgccgagca ccgcgcccca gacgtagccg acgccgccga tcaccgccat
    31981 gaacaggtat tcgatgccct gttcgacgct gaagggatag gggttgatga agcgctggaa
    32041 gtgcgcgtac agccagcccg aggcgcaggc ctgcagggcc gcgatgacga agatgaccat
    32101 gcgcgcctgg gccgtgtcca cgcccatgga ctcggccatc acgcggccgc cgcgcagggc
    32161 gcggatggcc cggcccatgc gcgagtccag caggttctgg gtggtccaga cgccgagcag
    32221 caggaagagc cagatcaggt agtagatctg gccgccctgg gtgagcttcc agccgaagag
    32281 cgagaccggc gggatgtcgg tcaggccggt atagccgccc agcccgtcga ccgtgctgaa
    32341 ggcatagaac aggctcaggc cccaggccag cgtgcccagc ggcaggaagt gcccggacag
    32401 ccgcagggtg atggcgccca gcacataggc cacgaccagc gtgagcagca tgccggccac
    32461 cagccccgcc cagggcgtgc ccagccaggc cagcccgccg ggcaggtcgc cggcatgcag
    32521 gcagagcacg gtggaggtgt aggcaccgat gccgacgaag gccgcctggc cgaaggaggt
    32581 caggccgccg acgccggtga gcagcaccag gccgatggcc accagcgcgt acagcccgat
    32641 gtagtcgagc agggtgacgt agaagggcgg cagcaagagc ggcccgagcg ccagcaggac
    32701 gacgaagagc gcgatgaggt gacgtggcga caacttcatt ccccatcctc ctcttcctcg
    32761 acatggcggc tcttgagcga gcgccagagg agtacgggga tgatcagggt gaacacgatg
    32821 atttctttgt aggcactggc gtagaacgag gaaaacgatt cgatcaggcc caccagcacc
    32881 gcgccggccg cggcgaccgg gtagctgacc agcccgccga tgatcgcgcc cacgaagcct
    32941 ttcaggccca gcaggaagcc cgagtcgaag tacagcgtgg tgatgggggc gatgagcagg
    33001 cccgagatgg cgccgatcag ggcggcgaag aggaaggtga tcttgccggc gaagggcggc
    33061 gagatgccca tcagcctggc gccgatccgg ttgtacgcca cggcgcgcag ggccttgccg
    33121 tacatggagc ggtcgaagaa caggtagagc gcgatgatga gcaggatgga taccagcacc
    33181 acgcaggcgg cctggccgtt gatgcggagg tcgcccagcg acagcgtggc gtcgatgaac
    33241 ggcgtggtgc ggccgccttc ggcgccgaag gccagcaggc ccaggcccag cagcgccagg
    33301 tgcagggcga tcgacacgat gagcagcacc agcggcgacg cggtggcgat gggctggaag
    33361 aacaatcgat aaatctgcgg acccatgggc acgaccaggg ccagcgtgag cagggcctgg
    33421 acggacatgg gcaggttggc cagcggcagc gtgtagaaca gcaccgccag cacgaaggga
    33481 taggccagcc tgaacagcag cggcatgcgc aggtggatgc gacccgtccg cctgtgcatc
    33541 gcccacagat cggccagcgc ttcgcagacg gcgaaggcgg ccagcagcca taccagcggc
    33601 accggcttgc cggcctggat ggccgccatg ctcagcgcgc tgtaggacgc gaactcgccc
    33661 tgcgggatga acaacacccg cgtgacggta aagaccagca ggatggccag ggccaacagc
    33721 gcgtagatcg cgccgttggt caagccgtcc tgcccgagca gcagggcaat ctgtgcattc
    33781 atgcgatggc ctgggcggga ttacttgagg agcgtccagg cgccgttctt gaccgtgatc
    33841 agctcgcggc cgcgttcgtc gaagccgctg tggtcttgcg gcgtcatgtt gtagacgcct
    33901 tgcgtgccga ccaggtcctt ggtctgttcc agggcgtcgc gcagcgcgct gcggaactcc
    33961 ttggtgccgg gcttggccga cttctcggcg atcgggatgg ccttttccag cagcaggccg
    34021 gcgtcgtaga cgttggcgcc gaaggtggcc ggctcgttgc cgtagagttt cttgtagtcg
    34081 gcgatgtatt ttgcggcgac ggccttggac gggttgctgt cggggatctc gggcagcacc
    34141 agcatcaggc tggcggccag gatggtgccc tcgacctgct tgccgccctg cttgaggaag
    34201 gcgtccagcg cggcgccgtg ggtctggtag aactggccct tgtagccttg gtcgaccagg
    34261 gtcttctcgg gcaggatgct ggggccgccg ggggcggcca ccagcacggc ctgcgggttg
    34321 gcggcgagga tcttcaggga ctggccggtg accgagttgt cgtagcgggt gaagcgctcg
    34381 ttggcgacga tcttgatgcc ggccttgtcg gccaggccgc tcacgacctt gtaccagttc
    34441 tcgccgaagg ggtcggagag gccgatgaag cccagcgtct tgatgccggt cttggccatc
    34501 tggtcgacca gcgccttggc gatgatgtcg tcgttctgtg tcgtcttgaa cacccacttc
    34561 ttctgctcgg tcatgggcag caccacgtcc gcgccgccca ccggggccag catgggcacg
    34621 ccggcctcgg ccacgaactg gatcacgccc atggcgttgg gcgtgcccgt gggtccgatg
    34681 atggcgtcga cgtgctcctc ggagatcagc ttcttgatgt cggtgacgga agcggtggtg
    34741 tcgctgccgt catcgagcga gatgtactcg accgacaggt caccgatctt ggtcggcaag
    34801 agcggcacgg agtttttctg cggaatgccg accagcgccg tggggccggt cgccgaggtg
    34861 atcaccccga ctttgacctg cgccatggcc ggcagggtaa acagggctgc aacggccgcg
    34921 gcggccagcg tcagttttgt gcacttcatg acaaatcctc cggtctctcg gaaagtgggt
    34981 actgcgatga cgggaaaaag caaaatcagg gcttgccggg caccagggcc agcacgcgca
    35041 ggggactgcc cgaaccatgc cggattttca gcggcgcgga gaagattacc gcgccggtgg
    35101 gcggcagctg gtcaagattg gtcaggcact gcaggccata gcggttgttt ccatgcatga
    35161 aatagtggca gggaagcggc gggtcgaggt gcgcggcctg gccggcgtcg gtgccgaccg
    35221 attcggtgcc gaagccgtgc acattgcgct ccttgatgag ccagggcacc acctcggcgt
    35281 ccgggccggg cgtgtgctgg ccgtcggcct gcatgttcag gtagtccacc gatttttcgc
    35341 gcttggacca gtcggtgcgc atcagcaccc aggcgccgtc cgggatcttg ccatgctgtt
    35401 cttcccactg cttgacgtaa tcgatgccga gcaggaagtc ggggtcctgg ccgctgggct
    35461 cggagcagtc gatcacgcag gcgctggcga cgaaggccga cagcgggatg gtgtcgacgg
    35521 tgttgtcggg ctggtccttg cccgagaccc agtgcaccgg cgcgtcgaag tgcgtgccgg
    35581 tgtgctccga gcacgagaag ttgttccagt accaggccgg gccgcgttcg tcgtagcgcg
    35641 agatttcctc gatgcggaag ggccaggcct ggccgaactc cggcggcagc acgatggtgg
    35701 gaaattcggg cgtcagcgtt tcggtgagat cgatgatgcg gatcccgccg ctggccagcg
    35761 cgcccatgaa ttgggcgagg attttggcgc tcatgtcagc tccgccgggc cgctcccaag
    35821 gagctgacgc gcccccttgg ggggcagtga acgtagtgaa cgtgggggtc gtttcatctt
    35881 ttttcctata ggcgtggccg ggctcaggcg cccagttcgc gctccagtcc cttggggttt
    35941 tcggcgaggc gggcgcggaa ttccttggcc acgtcgccca catagacttc ttccagtccg
    36001 tccttgagcg cgccgacgat ggcctgggcc accgccttgg gggccacgcg cggcggcggg
    36061 gtgagctgct cccattcgtg gtcgagcggg ccggggaaca cgttgaccac gcgcacgccc
    36121 gacggccgca tctcggcgcg caggccttgc gccagtgaca gggcggcggc cttggacgcc
    36181 gaccagatgg cgcgcgaagg caggctcatc tgcgcgtaga tcgacagcac gttgacccag
    36241 gccacggcat tgcgcgcgcc gtcggcggcg cgggcgcaca tggccgggcc gaaggcgtgc
    36301 gcgaggcgca tcatgcccag cacattgatg tcgagcaggt tgcgcgcgac gttgacgtcc
    36361 ttggtgtgca gcaggccgcc ttcgcgttcc atgtcggcgg tgttgaccag gatctcgacc
    36421 ttgccgccga attcgccggc ggcgcggtcg agcgaatcgg tgtcggtgac gtccagcggg
    36481 atcacgctca cgcgcgggtc ggcggcgaag gcgtcgaagg ccgcttcctt gcgccagcgc
    36541 tgcgggtcgc cgatatagac ctgcgaggcg ccggcatcga gcagggcctg ggccacggcc
    36601 aggcccacgg ccgatttgcc gtcggtgatg agggcgcggc ggaacttcgg gtcgcaggac
    36661 gtttcgcgca ggatcttgtc gtcttccata tggggcgtgt tccttgcagg cagggccagc
    36721 atgaccgcct ggcccgagcg gtcgagcttg atggccaggc ggacttcgtc gccgtcggcc
    36781 aggtcgccgt gcacgtgggc caccacggac gggccggcct ccatgcgcac ggtgccgatg
    36841 cgccagggca ggcgttcgcg gaaatacagg tcgttgctgt ggtgcagggt ggtgctgacc
    36901 agcagccggc ccttgggcga gacgggtttc cagggcaggt gctcggacag gcagtgcacg
    36961 cagacttcgc gcggcgggta ttgcacagtg ccgcattcgg cgcattgctg caattcgaaa
    37021 cgaccctcgg ccgcggcggc ggtcaggccc agcgcgctgc ggcttctcgc gccgggcggg
    37081 cgcgtgggct ggcggctgcg ggcgaccggg tctttcttgg gcggctggcg cagggcttct
    37141 gtcatcgtag ggcccccttg gcgccgtgcg ccgtcccccc gagggggagc aatgcgcact
    37201 tgaggcggct cggcgtgctc attgcgcgct ccccagaatg gcggcggcgg tgcacaggcc
    37261 gcggtcgtag ttgatcatgc cgaagccgct caccaggccg acgcgggcat cggccacctg
    37321 cgtcctgccg gccgtgttgc tgagctggcg cagcgcctcg accatgccca ggtagccgcc
    37381 ggcggcgccg gcctggccca ccgacagctg gccgccgttg gtgttgaaag gcagggtgcc
    37441 gtccaccgtg aaggtgttgc ggcgcacgta ctcggccgcc tcgcccttgg cgcacaggcc
    37501 caggtcctcg atctgcatga agttgatgac ggggtagtcg tcataggcct gcatgaagtc
    37561 gaggtcggcg ggcgtggcgc cggcctgggc gtatagctca tctatgtcca gggtccagcc
    37621 gccgcgcgtc tggatggggt cgtcgtgcca ggcgttgtgg cgttcgatgg tgcccaggat
    37681 gcgggcgtag ggcaggccca ggtcgcgggc gcgcttttct tccatgacca ggaagccttc
    37741 ggcgccggcg cagggcatca cgcagtcgaa caggtggatg gggtcggtga tgacgcgcgc
    37801 gtccatgtac tgctgcagcg tcagcggttt tttcatgagg gcgtgcgggt agcgcagcgc
    37861 attgtcgcgc tgggccacgc acagcttgcc gaagtcctcg cgggtggcgc cgtaggtgcg
    37921 catgtagtgg gtcgtgagca gggcgaagct ggcgttgggg ccgccggcgc cgtagggata
    37981 gacgccgtcc tgcgcgaagc gcgagaactg gctgaccgtg ttgcggaagg agtcgacgtg
    38041 gttggcgtcg cccgagatgc aggccaccac gctggcgtcg cccgcctgca cggcgcgggc
    38101 cgcgcggcgc aggcccacca cgccgctggc gccgcccatg ggaatgtggt cgagccagcg
    38161 cgggctcatg cccaggtgct gcatcaggcc cacggcggtg tcgggggcga gcgtgaagct
    38221 cgacacgcag acgccgtcga tggcatcctt gggcaggccg gactggcgca ccaggccgcc
    38281 cagggcgcgg cccagccacc agtgcgcggc atgcgtcgaa tagcgttcgt aggggatggt
    38341 gacgggcagg gccgccacca cgccgtcgta gctcaggcga gaagccatca gtggccgccc
    38401 tcctggcgtt ttttaagggc ggtggtgtcc acgcaggcgg gttggccgac cagctcgggc
    38461 gccagctttt tcatttcgcc gcgctggatc ttgttggtgg gcgtgagcgg cagagcgtcg
    38521 acgaaggcca cgtagccggg caccttgtag taggccagct cgcccaggct ccatgtcacc
    38581 agttcgcgcg cggcggcttc catggcctcg cgcgaggccg gcgtggtctc gggcacgatg
    38641 caggccagca cctcgtcgcc gcggatggcg tcgggcacgg cggccacggc cacggccttg
    38701 accagcgggt gggtgagcag gatggtttcg acctcgacgg ccgagatgtt ctcgccgctg
    38761 cggcggatca cgttcttctt gcggtcgacg aagcggatca tgccgtcggc gtcgcggcgc
    38821 acgatgtcgc cggtgtggaa ccagccgccg gcccagactt cggccgtcgc ctcggggtct
    38881 ttcaggtagc cggcgaagaa gccgaactgc ggcttggcgc cggcgtggcg caccagcagt
    38941 tcgccgggct cgcccggggc ggcctcggcg ccgctgtcgg ccacgatgcg cacctcgacg
    39001 tcgggctcct gcgtgccgat gctgctggtg cccacgaaac gcggctcctt gctggcgatg
    39061 atgacggcgc cgttgccggt ttcggtcatg gcccaggctt cgagcagggg gaagccgaag
    39121 cggttttcaa actcggcatg cagcgagcgt tcgacgccgg cgccgaaacc gaagcggatg
    39181 gcgtggtcgc ggtcgccggg gccgggcgcg gccttcatca ggatggccgg catcacgccc
    39241 aggtagtgca gcacggtggc gcgcgagcgg cgcacgctgt cccaccaggt cttggggtgg
    39301 aagcggtcga ggatgatcag gcagccgccg gtgaccacca tggccagcgt cgagcaggcc
    39361 atggcgttca tgtgcaccag gggcagcggc gtgagcatgc gctcgacgcc tgcgcgcagc
    39421 gtgcacaggc cgccgatgtt ggcgtaccag cggccgcaat gcaggaagta ttcgttgggc
    39481 aggatgcagc ccttgggccg gccggtggtg cccgaggtgt agagcagggc gcattcggtg
    39541 cggtcgtcgg gcgagccggc caggggcgcg gcggtcagcg cgggcggcgg ggcctcgtcg
    39601 ggcgtcatgg tgcgcagcgc gcggccggcg cgctgcgcag cctcttgcag gctggcgtgg
    39661 cggccgggca gggccacggc caggatgagt tcggaatggc cgatcaggta ttcgagttcg
    39721 gcggcgcgca ggtcgatatt gatgggcacg accgacacgc ccagcgcgtt cagggcaaac
    39781 cagtgcagga agaaggccgg gcggttttcc agcagcaggc cgacgcggtg gccatggccg
    39841 tagcccgcca cggcataggc gccgcgcagg cgttcgatcg tcgcgaaggc gtcgtcatag
    39901 ctgagctcgc cggcttcgat gccgtaggcc tcggcggttt cgggcagcgt gcacaggaat
    39961 gggttcttgc cccatttggc ggtggtgtca cgaaaagctt cgtagacggt cttggcgctc
    40021 acaggcgtcc cctcggtaca gaaaatgctt acatcaaaag ctggatattg ccgaatgcgg
    40081 cttcgcagtt gaccaggtcg acctgcttga agcggatctt cagcgcgccg ccggtctcga
    40141 ccaggtgatg cgtggcccag ccggcgtaca gcgtctgctc gtcgttgcgc gtctccacat
    40201 agtggaaggc ggtgcgcgtc acgtagcggc cctcggcggg ctggtgctcg gcatggccgt
    40261 gttcgacgaa cggcgtctgc agcaggtggt ggcagcggct cttgggctgc tgcgagaagg
    40321 tgcgctggcc ggccaggcgt tcgacgcgca cgcgcagcag cagcttgttc tcgtgcatca
    40381 gcgacgtatg caggcgcgca tcgggctggc cgtgcgtgag cggcatccag taccaggcgt
    40441 cgtcgtcgta gaggtcgagc cagtcttcga atttttgttc gtcgatcagg cgggcttcct
    40501 ggtagatgaa ggcgatcagc tgcgggtcgg tcatcattgc gcgccctccg tcatgtagcg
    40561 ggcccaggcg cggtattggt tgcgcatctg ccattcgttg gtgccgttgg tgaccacgtt
    40621 ctcgcgcccg gcctcgccgg gttcgtagag gcgggccacg ttgacccatt cgcgggcgcg
    40681 ggtcctggtc aggccctgct gggcgcgttc gtacatttcg agatcgtcgt ggccgaccat
    40741 ggaggtcggc gcgttgatca ggcggttgta catgatggtg cgttccagca gcatgtcggg
    40801 cgcgcccacc aggcggaacg tccacgactc caccagggtg cggtcggccg ccaggggctt
    40861 gaacacgcgc agggtctgga tggggccctt gaccatgatg tgcgggaaga aagtggtgtt
    40921 gtggcgcgat tcgccgagga tctggtgcgc gcgctcctcg ccgtaggcgg cgaccatcga
    40981 atcccagtag ccgggaatgt tcgagtaatt ggagtggatc gagcccgaca cgccggtgtg
    41041 gccgtggccg ttcttccaga cccggatgcc ggtgttttcg tagaactcgt agggcgacat
    41101 gaaaggcgcg aagagttcga cggccatggg cttgggcgtg ccttcgggcg cttcctgcca
    41161 gacgcgcacg gcggtgccgg ccgacgactc gtgggccacc atggggtggc aggtgtcgga
    41221 ctggttgtcg accagcatct tccagttgca gcggtgcatg tagcgcagca cgccgccggc
    41281 cacttccagc ttgcccacgg gcgagcggtc gaccatgttg tcgatggtgg agagcgattc
    41341 gccgaagaag tcctcgaacg actcgccggt ttcgctcagg cggcagaaca cgaagccgcg
    41401 gtagttgcgc acggcagaga ctgccgccat gccgcggctg gcctcgcact gctcgaagcc
    41461 ggtgttctcg tagcctttct tgagcggcac cgacagcagg ctgccgtcgg tcttgaaggt
    41521 ccaggcatgg taggggcagc ggaagaactt gccggtattg cccgagccgt cggcggccac
    41581 ctgcacgccc tt
//
LOCUS       DASWPO010000086         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_107782, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000086 DASWPO010000000
VERSION     DASWPO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107782"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VGC69_05185"
     CDS             <1..443
                     /locus_tag="VGC69_05185"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294715.1"
                     /translation="LRSSEVRVFQPVGDARGQPLMAFYTYYVLRPMLAYFSKRTQKAD
                     AKGNIVSDHSDTFTTLLTVQPIDDKTSTVRVLAALNVQPHPDPEKVIERADVVFNQDR
                     EIVETQRPERIPTDLRLELHHRTDLMGQRYRSWLRGKGITYGVI"
     gene            430..1395
                     /locus_tag="VGC69_05190"
     CDS             430..1395
                     /locus_tag="VGC69_05190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY0294716.1"
                     /translation="MASSEPGSLSLRLAGIEYGAEGVNIYRFASVDGAALPGFEPGAH
                     VDVHIGPALMRQYSLLWPAPAPDAYAIAVQVDMAGKGGSRALHHESVVGHRYALSAPR
                     QHFGLAEAGAAGHWLFAGGIGITPIISMYRKLRRAGQPARLHYWAKTPGQMLFFDELR
                     AAGGEVRLFHTEADTRAPRLADILAEVPADAQLYCCGPAGMIDAFDAACARRPAANVH
                     RERFSAALPEDLGPEDAFRVTLRKSGKVLTVQPDETLLGACLAAGVDVSYSCEEGVCG
                     ACEVKVLAGAVIHRDSVLSPAAQAQSASMMICCSRGVGSSLELDI"
     gene            1417..>2097
                     /locus_tag="VGC69_05195"
     CDS             1417..>2097
                     /locus_tag="VGC69_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0294717.1"
                     /translation="MQAGLRVGIIGGGIGGVALARALDAAGIEYRLFERVAAFGEVGA
                     GVQMTPNAVKVLKALGLAEDLRRMGFLPEAMVGRNWETAQELFRTPLKTTCPALFGAE
                     FYHVHRADLHTALAKDIPAGNSALGMQCTGIGQEGDRAVATFSDGSRFEADLIVGADG
                     IHSVVRNALWGTEPAHYTGHMCWRALVEVDRHPLPFVSPDSAFWMGPKGHVVTYYVKG
                     GAAVNIVAV"
BASE COUNT          310 a          700 c          731 g          356 t
ORIGIN      
        1 gcctgcgctc gtccgaggtc agggtgttcc agcctgtcgg cgatgcgcgc ggccagcccc
       61 tcatggcttt ttatacctac tacgttctgc gcccgatgct ggcgtacttt tccaagcgca
      121 cgcaaaaagc cgatgccaag ggcaatatcg tgtccgacca ttcggacacc ttcaccaccc
      181 tgctcaccgt gcagcccatc gacgacaaga ccagcaccgt gcgggtgctc gcggcgctca
      241 atgtgcagcc gcatcccgat ccggaaaagg tgatcgagcg cgccgacgtc gtgttcaacc
      301 aggaccgcga gatcgtggaa acgcagcgcc ccgaacgcat cccgaccgat ctgcgcctcg
      361 agctgcatca ccgcaccgac ctcatgggcc agcgctatcg ctcgtggctg cgcggcaagg
      421 gcatcactta tggcgtcatc tgagccgggc agcctgtcgc tgcggctggc gggcatcgag
      481 tacggcgccg aaggcgtcaa catctaccgt ttcgcctcgg tggacggcgc ggcgctgccg
      541 ggtttcgaac ccggggcgca cgtggacgtg cacatcgggc ccgcgctgat gcggcagtat
      601 tcgctgctgt ggcccgctcc cgcgcctgat gcctacgcca ttgccgtgca ggtggacatg
      661 gcgggcaagg gcgggtcccg ggccttgcac catgagtcgg tcgtggggca tcgctatgcc
      721 ttgtcggcgc cgcgccagca tttcggcctg gccgaggccg gggcggcggg gcactggctg
      781 ttcgccggcg gcatcggcat cacgcccatc atttccatgt accgcaagct taggcgggcg
      841 gggcagccgg cgcggctcca ttactgggcc aagacgcccg ggcagatgct gttcttcgac
      901 gagctgcgcg cagccggcgg cgaggtgcgg ctgttccata ccgaagccga tacccgcgcg
      961 ccgcgcctgg cggacatcct ggccgaggtg ccggccgatg cgcagctgta ctgttgcggc
     1021 cccgcgggca tgatcgatgc gttcgacgcc gcttgcgcgc gccggcccgc ggcgaacgtg
     1081 catcgcgaac gcttcagcgc cgcgctgccg gaagacctcg ggccggagga cgcctttcgc
     1141 gtcaccttgc gcaagtcggg caaggtgctg acggtgcagc ccgacgagac gctgctgggc
     1201 gcctgcctgg cggccggcgt cgacgtgtcg tattcctgcg aggaaggcgt gtgcggcgcc
     1261 tgcgaggtca aggtgctggc cggcgcggtc atccatcgcg actcggtgct atcgccggcc
     1321 gcgcaggcgc agtcggcttc catgatgatt tgctgttccc ggggagtggg gagctccctg
     1381 gaactggata tttgaaaaat agacgaggag ttcactatgc aagcaggctt acgcgtcggc
     1441 atcatcggcg gcggcattgg cggcgtggcg ctggcccgcg cgctcgatgc ggcggggatc
     1501 gagtaccgcc tgttcgagcg ggtcgcggct ttcggcgagg tgggcgcggg cgtgcagatg
     1561 acgcccaatg ccgtcaaggt gctcaaggcc ctgggcttgg ccgaggacct gcgccgcatg
     1621 ggttttttgc ccgaagccat ggtcggccgc aactgggaaa ccgcccagga gttgttccgc
     1681 acgccgctca agaccacctg tccggcgctg ttcggcgccg agttctacca cgtgcaccgg
     1741 gccgacctgc acacggcact ggccaaggac attcccgccg gcaactcggc gctgggcatg
     1801 cagtgcacgg gcatcggcca ggagggcgac cgcgccgtgg ccacgttctc ggacggcagc
     1861 cggttcgagg ccgacctgat tgtgggcgcc gacggcatcc attcggtggt gcgcaacgcc
     1921 ctgtggggca cggagccggc ccactacacc ggccatatgt gctggcgcgc gctggtggag
     1981 gtcgaccgcc atccgctgcc cttcgtcagc ccggattccg ccttctggat gggccccaag
     2041 ggccacgtgg tgacctacta tgtcaagggc ggcgcggccg tgaacatcgt ggcggtc
//
LOCUS       DASWPO010000087         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2609145, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000087 DASWPO010000000
VERSION     DASWPO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609145"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1343
                     /locus_tag="VGC69_05200"
                     /pseudo
     CDS             99..1343
                     /locus_tag="VGC69_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939933.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
     gene            1793..2065
                     /locus_tag="VGC69_05205"
     CDS             1793..2065
                     /locus_tag="VGC69_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0294718.1"
                     /translation="MNKTELIDHIASKADISKAAAGRSLDALIGAVKTTLKKGGTVTL
                     VGFGTFAVSSRAARTGRNPRTGETIKIKKAKVPKFRPGKALKDAVN"
     gene            2197..2272
                     /locus_tag="VGC69_05210"
     tRNA            2197..2272
                     /locus_tag="VGC69_05210"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2230..2232,aa:Val,seq:tac)
     gene            2414..>3075
                     /locus_tag="VGC69_05215"
     CDS             2414..>3075
                     /locus_tag="VGC69_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294719.1"
                     /translation="MKLIIRVVPPAPRKTLRSFMSALYDSSDFSVSQTPMALRVGAGV
                     LVLALHVAFIAAFVLGKKVDKLELPEEPAVMVSVIDAPAPQQAKAELQPETVQPPPQP
                     PAPVDPPPVPPVEQPPVDPPPVEPDPEPDPPPVVHPEPLAPVIKPHPKPPVKPPVKQQ
                     PVKAQPVPPAPTPPAPAAVQAPSGTPDGQQQVSQAPRQADPVMVNSVEYDCARPMPVY
                     PTA"
BASE COUNT          576 a         1051 c          903 g          545 t
ORIGIN      
        1 ccgagcatcg gaattccgca gggcagcctc ggcggctcaa gaactcaaat atcgctatca
       61 aagtagtaaa gaaagactag ccagagacga aacacaccat gcataaaaac ggcctcacca
      121 cccaaatcct gcacgccgac cgcaagcaat ccgtcgagca cggcgccgtg cacaagccca
      181 tgcacccttc gtcggagtac atctacgccg atgcccgcga actggccgcc gtgttccagg
      241 gcaagtccgg ctatacctac gcgcggcagg gcacccccac caccaacgcc ctcgaagcca
      301 agatcacgct gatggaagag ggcgtcgcat cggtgagctt cgccaccggc atggcggcgc
      361 tcacggccgt gttcaccacc ctgctgcgcc gcggcgacca cctggtttcc agccagttcg
      421 tcttcggcaa caccaacagc ctgctgggca cgctgatcga actgggcatc gacatcacct
      481 acgtcgacgc caccgacgtg gcacacgtgc gcgccgcgct gcggcccgag acccgcatgg
      541 tgttcaccga gaccatcgcc aaccccggca cccaggtggc cgacctcgag gccatcggcg
      601 aactgtgccg tgaacgctga ctggtctata tggtcgacaa cacgctgacc tcgccttccc
      661 tgttccgtcc gcgcgacgtg ggcgcttcgc tggtcatgaa ttcgctgtcc aagtacatcg
      721 cgggccacgg ccatgcgctg ggcggcgcca tcaccgacac gggcctgtac gattgggccg
      781 gctacgccaa tatccacgac gcctatcgca aggggccgtc gaccggctgg ggcctgctgc
      841 agatcaagaa gaagggcctg cgcgacatgg gcggcacctt ggcggccgaa ccggcgcacc
      901 gcatcgccat cggcgccgag accctggcgc tgcgcatgca aaagcattgc gccaacgcgc
      961 tggcgctggc gcgcttcctg gaacagcatc ccggcgtggc caaggtgcat taccccggcc
     1021 tggccggcca cgcgcagcat gcgcgcgcca ccgagttgtt cggcggccgc tacggtgcgc
     1081 tgttgggcat cgagctggcg gacggcatcg actgcttcga cttcctcaac cgcttgcagg
     1141 tcgtgctgat ggccacgcat ctgggcgata cgcgcagcct ggcgctgccg gccgcgcaca
     1201 ccatctacta cgagatgggc cccgagcgtc gcaagcagat gggcatctcc gacagcttca
     1261 tccgcctgtc ggtgggcatc gaggacgaag ccgatctgat ccacgatttc gaccaggcct
     1321 tgcaagccgg cacgccgcgc tgatgcgggc ggggcatccg tcccttctgt agattgcgga
     1381 cgtttatcac aagctgttgc gcagttgggt cgagttgtag ccaaagcgtt cataagcaat
     1441 gcaaagtgcg gtgcatgtcc cgcacgcgcc cgcctcggta acccgcgcat ctggtcttgc
     1501 ggcattgcgt catatgcagg aaagccagtg atgacgcggc tcatagccga ttcattaggg
     1561 ttttatgctt gacaatgaac gctgacgcaa tgcctaatac agtcactgcg tcgcagtgat
     1621 ttcgatgcgt ttattccgtc gaagccgctg cgacgtagca attagaacgc gcgctttgtc
     1681 gcgcagcctt ccattctttc ttcctggtca gttcacgtct ttgccggtca gtggcgcgtg
     1741 tcggccatct gcgctctacg tcagctatct actttgtgag gggtaaaact taatgaacaa
     1801 aactgaactc atcgatcaca tcgccagcaa ggccgacatt tccaaggctg cggcggggcg
     1861 ctcgctcgat gccctgatcg gtgccgttaa aaccaccctg aaaaaaggcg gtacggtaac
     1921 gttggttgga tttggtacgt tcgccgtctc gtctcgcgcc gcgcgcacgg gccgcaatcc
     1981 ccgcaccggc gaaaccatca agatcaaaaa ggccaaggtg ccgaaattcc gtccgggcaa
     2041 ggcgctcaag gacgccgtca attgatccct cggtcgagca cagcgctcac cagcagtaca
     2101 acaatcgagg gcacatgccc tcgatttgct ttgggggcag cgcgatggct atactgccgc
     2161 ctcgcaagat gcccaggcgt ggccccgtat gacttcgggt gcttagctca gttggtagag
     2221 cggcgccctt acaaggcgta ggtcacaggt tcgacccctg tagcacccac cacttcctca
     2281 tgcatcggca agcgcgggca ctcgtgcatc gctcatcgaa acggctgcgg caacgcaagc
     2341 cgttttttta ttgccgccac cccctgttca gggtagtttt gaacttgcga atcattcgca
     2401 tttaagatac tgtgtgaagc tcatcatccg cgtggtgccc ccggcgccgc gtaaaacgct
     2461 gcgcagtttc atgtccgccc tctacgattc ttcagatttt tcggtttcgc aaacgcccat
     2521 ggcactgcgc gtgggggcgg gcgtgctggt gcttgccttg catgtggcct tcatcgccgc
     2581 tttcgtgctg ggcaagaagg tcgacaagct cgagctgccg gaggagcccg ccgtcatggt
     2641 gagcgtgatc gatgcgcccg cgccgcagca ggccaaggcc gaactccagc cggagacggt
     2701 gcaaccgccg ccccagccgc cggcgccggt cgatccaccg ccggttccgc ccgttgaaca
     2761 gcctcccgtc gatccgccgc cggtcgagcc cgaccccgaa cccgatccgc cgccggtcgt
     2821 gcatcccgag cccctggcgc ccgtgatcaa gccgcatccc aagccgccgg tgaaaccgcc
     2881 ggtcaagcag cagcccgtca aggcgcagcc tgtcccgcca gcgccgaccc cgccggcgcc
     2941 cgcggccgtg caggccccgt ccggcacgcc cgacggccag cagcaggtca gccaggcgcc
     3001 gcgccaggcc gatccggtga tggtcaacag cgtcgagtac gactgcgccc ggcccatgcc
     3061 ggtctatccc acggc
//
LOCUS       DASWPO010000088         8649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2697386, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000088 DASWPO010000000
VERSION     DASWPO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697386"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VGC69_05220"
     CDS             complement(<1..727)
                     /locus_tag="VGC69_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0294720.1"
                     /translation="MQQSPVFYRDRPKMHMDPLSEVLSFLSTQSSFFAGLGAGGAWAI
                     SFPPPAGIKFNAVVDGTCWLAVEGQEQPIRLEAGDCFLLSRKRAFSLGSDLSLPPVKS
                     DEVYRHAVNGIARHGHGDEFFLIGGHFAFSDEAHRLFDGLPAVAVIKNHSDQASVLRW
                     ALQRLAHELTSPSPGSALVAQHLGHIMLVQVLRIYLAQEGNSTPSWLLALSDPRIGAA
                     IQAIHANPERRWTVEDLAEVAGVS"
     gene            806..1768
                     /locus_tag="VGC69_05225"
     CDS             806..1768
                     /locus_tag="VGC69_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294721.1"
                     /translation="MPHSQTPIHSGFDATTTAAHVLQGRDLSKVAAIVTGGHSGLGFE
                     TTKALANAGAHVIVAARDPDAARAKVGEIGNVEVEQLDLSDLASVRAFATRFLATGQH
                     IDILIGGAGMMACPQTRVGPGWEAQFATNHLGHYALVNLLWPALQGGARVVAVSSGGH
                     RWSGIRWDDVQFERGYDKWLAYGQSKTANALFAVHLDKLGQCAGVRAFSVHPGKIFTP
                     LQRHLSQEEMIVAGWLDANGKPADPTFKTAQQGAATQVWAATSPQLQDMGGLYCEDCE
                     IAPLDESEPSACVGVRPYAVDPAQAERLWALSAKLTGIDAFAAK"
     gene            complement(1841..2620)
                     /locus_tag="VGC69_05230"
     CDS             complement(1841..2620)
                     /locus_tag="VGC69_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0294722.1"
                     /translation="MSPRTARIEGDPPNPVSFRTEVFKAGTVFPRKRQAWGELNYALS
                     GVCEFEIDGARYLSPPHYGIWIPPGVPHEAWNRHDIRYVCVYVERRLCKNLPSTPCTL
                     GLSLLLKAILADFDEREVTLPATREDLRLAQVLVDQIRLAPRFESYLPMPEDALLVSI
                     LHRLQADPGNRISAARLAGEAGTTERTLSRRCQEQLGMSFNEWRQRLKLVSALALLDE
                     GEPVFRIAQRLGYGNASAFITMFRRMTGTSPMQARRREGRM"
     gene            2731..3660
                     /locus_tag="VGC69_05235"
     CDS             2731..3660
                     /locus_tag="VGC69_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0294723.1"
                     /translation="MRTPATHTVPITFRSALMPLATVLIWSVNTVVTKASSSVISPSS
                     ISFYRWLLAFVVLLPFVGRAAWRERAVAMAHWKQLAALGAMGMVIYQCLAYEAAYSTT
                     AVNMGVIVALMPLFSTLLASILASERLTFARVAGAIVSLAGLVYLTSHGDPAGLLHGE
                     LHVGDALMLVAVVSNSLYGVMLKRWAIPLSVIQQLLWQIGFSTLMLLPVWLLGAISPI
                     TSANLPLILFAAIPTSLLAPLCWMIGVRQLGAARSALFLNLLPVIVALLAWLWLGEHL
                     QVFHLIGGALALFGVSLGLGEWRWMRVRRSASS"
     gene            complement(3706..4362)
                     /locus_tag="VGC69_05240"
     CDS             complement(3706..4362)
                     /locus_tag="VGC69_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0294724.1"
                     /translation="MPQPTIKPARQAAKRPRGRPACNANPGSDMLLRHGRRAFAKQGF
                     EATSVRGIAREAGVDPALVTHHFGSKESLWTAIVEQIAEQAEPMIESTRELRTGNIPM
                     RQRVESAVSLLIDQVFAEPDIGMFFSTAATEQGDRLDFLIDRLVRPYHDAFVPLLQDA
                     MEAGELAPNNPEILFLMLTNAIGRTVAYSHVLSAFSTLPRRPGEFKKVVLRTALTLLN
                     "
     gene            4687..7053
                     /locus_tag="VGC69_05245"
     CDS             4687..7053
                     /locus_tag="VGC69_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0294725.1"
                     /translation="MNKVDPVRQIDFLLIGGGLASAKAAETLRREGAEGSILMLSAES
                     MLPYHRPSLSKRYLLGTVDEARILVHAEQFYREQNIEIALDTQATAVDTTRQEVRTST
                     GARLHYDKLLIATGSVPRPLAVPGLALQGVHMLRFKPDCDAIRRHAAKARRAVVVGGS
                     FQGMEVAMSLLELGLEVTIVEGGGRLMRHLESGTLSTFFQGYARQRGASVTVGDPVVA
                     FHGRRKVSEVQTQSGLKIACDLVVVCAGVEPATAFLEGSGIQLEQGRVVVDDLLAASV
                     PNVWAAGDVTSFLDPVFARRRHIEHWDNAVKQGYHAALNMLGRRMRYDEVSYFFCEVG
                     DMGFDMLGAVEETDEWIARGSLEERSFALFYLKDNVPRAMFSLGRPPGEVRLSEGLIR
                     YRTNVLPQKASLANPDFALDQLASQTVLILQGGGALGAFECGVVKALEEHRIFPDIVA
                     GISIGALNGAIIASNPGKSTAVLEAFWRDLQVASSPALPQPLRRAMTAMQILQFGVPN
                     FFRPRWLPMLGEPWTAPWAWTSFYDVSPMRRLIEKYVDFAALKSSPVRLLVGAVNVLT
                     AELRVFDSYVDDLTPDHILASGSLPPGFPWTFVDGVPYWDGGVISNSPLDLVVDRCGP
                     DGKRVFIVDLFAGQKALPANMMEILARRDEIVYSERVRSDLRQRELKDAYRDLVDSIL
                     QDVEPGQRDRIKLRPAYIQLMGDGAQMHITRFVRKGAPDEPSSRDYDFSDVSIQTNQA
                     QGYELARDTLNERAAKVEVREDLPADGRETGKFHRGNPPRRFEEWRNG"
     gene            7046..7516
                     /locus_tag="VGC69_05250"
     CDS             7046..7516
                     /locus_tag="VGC69_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294726.1"
                     /translation="MAEEKSNTMMQVYLANMRLMQRLGGSLQAAGMQWSQQGQTAATT
                     AAAEFSAELGELLNTKDFPGLLALQLNIVRRHWDQNQALLLQAFNLGAQNPELAAEFR
                     EAFGEWKQELAGITSEAGSVAQALTPWTQYLAQFQQLWNPEGGGAAAKGKPGRK"
     gene            complement(7565..>8649)
                     /locus_tag="VGC69_05255"
     CDS             complement(7565..>8649)
                     /locus_tag="VGC69_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294727.1"
                     /translation="AGIHALAGDARQAGQCRDRAYGLQRVFIEAAGQPRRRVLLLCAG
                     RAAGNVPFEVLVPTPDNCRIKYAIDYAEPAEDAALPSSDLAFNAIGEPDVAVPLAARL
                     HGFAQRYARPLLNAPGRVAATRRDRLPALLDDIAGLQTARCLRLDEAPNTPREALDAL
                     ARRLDEAGLALPLLVRPAASHGGAGLVRCTDQAEFDQALRGLQGPGYLSAYRDCRSPD
                     GHYRKYRVVYVDRRPYPYHLAISPHWMVHYHSAGMLAEPWKREEEARFLADPRTELGM
                     AAWAALEALGRQMDLDYAGADFTLLPDGAVFVFEANATMLVHYERPDGPLAHKNPYIR
                     RIADAFGRLLGAHCHAPGTGHPAGAT"
BASE COUNT         1425 a         2688 c         2931 g         1605 t
ORIGIN      
        1 gcgagacgcc cgcgacttcc gccaggtctt cgacggtcca gcggcgttcc ggattcgcat
       61 ggatggcctg gatggcagcg ccgatacgcg ggtcggacag cgcaagcagc cagctcggcg
      121 tgctgttccc ctcctgggcc aggtaaatcc gcaggacttg taccagcatg atgtgcccca
      181 agtgctgggc aaccaaggcg ctgccggggg aggggctggt cagctcgtgc gccagccgct
      241 gtaaggccca gcgcagcacc gacgcctgat cggagtggtt cttgatgacg gcgacggcgg
      301 gcaggccgtc gaatagacga tgcgcttcgt cgctaaaagc gaagtggccg cctatcagga
      361 aaaactcatc gccatgtcca tggcgagcga tgccgttgac cgcgtggcga tagacctcat
      421 cggatttgac cggcggcagc gaaagatcgc tgcctagcga aaaagcccgc tttcgggata
      481 gcaggaaaca gtcgcccgct tccagccgga tcggctgctc ctggccttcg acggccagcc
      541 agcacgtccc gtcgaccacg gcgttgaatt tgatgccggc cggcggcgga aagctgatgg
      601 cccaggcgcc acccgcaccg agacccgcaa aaaacgaact ctgcgtgctt agaaaggaca
      661 ggacttcaga cagtgggtcc atgtgcatct tcggacgatc tcgatagaaa acaggacttt
      721 gttgcattct cgatcctaag tctacctcgg ataatccaat gcacagcggt ctctggtttt
      781 gctccgacta ttcataggat cacacatgcc acactcgcaa actcccattc attccggttt
      841 cgatgcaacc acgacagccg cgcacgtgct gcaaggacgc gacctttcca aggttgcggc
      901 tatcgtgacg ggcggacact ccggtcttgg cttcgagacg acgaaggcat tggcaaacgc
      961 cggcgcgcac gtcatcgttg cggcgcgcga cccggatgcg gctcgcgcta aagtcggtga
     1021 aattggcaat gtcgaggtcg agcagctcga cctgtccgac ctggccagtg tgcgcgcttt
     1081 cgccacgcgc ttcctcgcaa ctggccagca tatcgacatc ctgatcggcg gagccggcat
     1141 gatggcgtgt ccgcagaccc gggttggtcc cggctgggag gcgcagttcg ccaccaacca
     1201 tcttgggcac tatgccctgg tgaacctgct ctggccggcc ttgcaaggcg gcgccagggt
     1261 ggtggccgtg tcttccggcg gtcatcgctg gtccggcatc cgctgggatg atgtgcaatt
     1321 cgaacggggc tacgacaagt ggctggccta tggccagtcg aaaaccgcca atgcgctctt
     1381 tgctgtccac ttggacaagc ttggccagtg cgcaggagtg cgtgcgttct cagtccatcc
     1441 ggggaaaatc ttcacgcctc tccagcgcca tctgtcccag gaggaaatga tcgtggccgg
     1501 ctggctcgat gccaacggca agccggccga tccgactttc aagacggcgc agcagggagc
     1561 tgccacccag gtttgggccg caacgtcgcc tcaattgcag gatatgggcg gcctgtattg
     1621 cgaagactgc gagattgccc cgcttgatga aagcgagccg tcggcttgcg tcggcgttcg
     1681 gccctatgcc gtcgatcccg cacaggcaga gcgcctttgg gcgctatcgg cgaagctcac
     1741 cggcatcgat gcctttgcgg caaaataagt ggcggcgata gcgctacgtt agttccgctg
     1801 cttcccatgc agcaggccta tatgtcgcgc ttgcggggct ttacattcgc ccctctcgcc
     1861 ggcgggcctg catcgggctg gtccccgtca tccggcgaaa catggtgatg aaggcggaag
     1921 cattcccgta tccgagccgc tgcgcgatgc gaaagaccgg ctcgccttcg tccagcagcg
     1981 ccagggccga caccagtttc aggcgctgcc gccactcgtt gaacgacatg cccagttgct
     2041 cctggcagcg ccgggacaag gtgcgctccg tcgtgcccgc ttcgcctgcc agccgcgccg
     2101 ccgaaatgcg gttgcccgga tcggcctgca ggcgatgcaa tatcgagacg agcagcgcat
     2161 cttcgggcat cggcagatag ctctcgaaac gcggggcgag ccggatctga tcgaccagga
     2221 cctgcgccag gcgcaaatcc tcgcgcgttg ccggcaatgt gacctcgcgc tcgtcgaagt
     2281 cggcgaggat ggccttgagc agcaggctga gccccagcgt gcaaggcgtt gaaggcaggt
     2341 tcttgcacag gcgccgctcg acgtatacgc acacatagcg gatgtcgtgc cggttccatg
     2401 cctcatgcgg gacgcccggc ggaatccaga tgccgtagtg gggtggggaa agataccgcg
     2461 cgccgtcgat ctcgaattcg caaacgcccg agagcgcgta gttgagctcg ccccatgcct
     2521 ggcgcttgcg cgggaaaacg gttcccgcct tgaacacttc ggtccggaac gagacggggt
     2581 tgggcggatc tccctcgatc cgggcggtgc gcggggacat gtccgagttt cgttatgagc
     2641 tggccgaaat ttactatata ccttgaatcg gccaaaaagt acgatggtgt ccatggcgaa
     2701 ccggtctcgg ttcgcccagt tggagattgc atgcgcaccc ccgcaacaca taccgtgccg
     2761 atcacgtttc ggtcggcctt gatgcccctg gcgaccgtcc tgatctggtc cgtcaatacc
     2821 gtcgtgacca aggcttcttc cagcgtgatt tcgcctagct cgatttcttt ttatcggtgg
     2881 ctgctggctt tcgtcgtgtt gttgcctttc gtgggccggg ccgcatggcg cgaacgcgcc
     2941 gtggccatgg cccattggaa acagctcgcc gcgctcggcg cgatgggcat ggtgatctac
     3001 caatgcctcg cctatgaggc cgcgtacagc acgacggcgg tgaacatggg cgttatcgtc
     3061 gccttgatgc cgctgttctc gaccttgctc gccagcatcc tggcgtccga gcggctgacc
     3121 ttcgcacgtg tcgcgggagc gatcgtgtcc ctggccggcc tggtgtacct gacttcccac
     3181 ggcgatccgg cgggcctgct gcacggcgaa ctccacgtcg gcgacgcgtt gatgctcgtt
     3241 gccgtcgtgt ccaactccct ctacggcgtg atgttgaagc gctgggcgat cccgctctcc
     3301 gtgatccagc agctgttgtg gcagatcggc ttttctacct tgatgctgct gcccgtgtgg
     3361 ctgcttggcg cgatttctcc catcacctcc gcgaacctgc ccctgatcct cttcgcggcc
     3421 atcccgacgt ccttgctcgc gcccttgtgc tggatgatcg gcgtgcgcca gctcggcgcc
     3481 gcccgcagcg cgctttttct caatctgctg ccggtcatcg tcgccttgct ggcatggttg
     3541 tggctgggcg aacacctgca ggtctttcac ttgatcggcg gcgcgctcgc gctattcggt
     3601 gtgagcctgg gtctaggaga gtggcgctgg atgcgggtgc ggcggagtgc gtctagttag
     3661 cagtcgtctg cccggaggcg aaggtgttta aagtgcgcgc atccctcaat tcaacaacgt
     3721 caacgccgtg cgcaacacga ctttcttgaa ctcccccgga cgccgcggca gcgtggagaa
     3781 ggccgacagc acgtggctgt aggcgacggt cctgccgatg gcgttggtga gcatcaggaa
     3841 caggatctcg ggattgttgg gggcgagctc acccgcctcc atcgcgtctt gcagcagcgg
     3901 cacgaacgcg tcgtggtagg ggcggaccag gcggtctatc agaaagtcga ggcggtctcc
     3961 ctgctcggtg gccgccgtcg aaaaaaacat gcctatgtcg ggttcggcga agacctgatc
     4021 gatgagcagc gacaccgcgc tctcgacgcg ttggcgcatg ggaatgttgc cggttctcag
     4081 ctcccgcgtc gattcgatca tgggttcggc ctgctcggcg atctgttcga cgatcgcggt
     4141 ccacagcgat tccttcgagc cgaagtggtg ggtcaccagg gcggggtcca cgccggcctc
     4201 gcgcgcgatg ccgcgcacgc tcgtggcttc gaagccttgt ttggcgaagg ctcgccgccc
     4261 gtgcctgagc agcatgtccg aaccgggatt ggcgttgcag gccggccggc cgcgcggccg
     4321 ctttgctgct tgccgtgccg gtttgatggt gggttgaggc atcgtgattt cctggaacgg
     4381 tcaacggtga ttcaaagcca ttgttcaggc tggcgccagc cgcgggactt tgtttcgcat
     4441 gggatgggct ttttgccttc ggctgcttta tctgcgcggg ttgaatcggt agaatgaccg
     4501 atagtaattc atcaggtgat gaatattccc atgtgcacaa gccatggcat ggccgcgcgt
     4561 ttttacttct ttcgcgggag tccgatcatg agcagtctga gtggcaaggt tgccgtcgtc
     4621 actggcgcgg catcgacctg gcagtcgttc gccgcgagcc gcggcggggt catgcaataa
     4681 atgcccgtga acaaggtgga tccggtgcgg cagatcgatt ttctcctgat cggaggcggt
     4741 ctggcgagcg cgaaggcggc ggaaacgctg cgcagggagg gggccgaagg gtccattctg
     4801 atgctgtcgg ccgaatccat gctgccttac catcgtccca gcctgtcgaa gcgatatctg
     4861 ctcgggacgg tcgacgaggc gcgcatcctc gtccacgccg agcagttcta tcgcgagcag
     4921 aatatcgaga tcgcgctgga tacccaggcg accgcggtcg acacgacccg gcaggaggtt
     4981 cggacctcga cgggcgcacg gctgcattac gacaagctgc tcatcgccac gggttcggtg
     5041 cccaggccgc tggccgtgcc tgggctggcg ctgcaaggcg tccacatgct ccggttcaag
     5101 ccggattgcg acgcgatccg gcggcacgcg gccaaggccc gccgcgcggt cgtcgtcggc
     5161 ggcagcttcc agggcatgga ggtggccatg tcgttgctgg agctcgggct cgaggtgacg
     5221 atcgtcgagg gcggcggtcg cctcatgagg cacctggaat ccgggacgct ttcgaccttt
     5281 ttccaaggct atgcgcggca gcggggcgcc tcggtgacgg tgggcgatcc ggtcgtggcg
     5341 tttcacggcc ggcgcaaggt cagcgaagtc cagacccaat ccggcttgaa gatagcctgc
     5401 gacctggtcg tggtttgcgc cggggtcgaa ccggccacgg cgtttctcga gggcagcggc
     5461 atccagctgg aacaaggccg cgtagtggtg gacgatctgc tggccgccag cgtccccaat
     5521 gtctgggccg cgggcgacgt cacgagtttc ctcgacccgg tctttgcgcg gcggcgccac
     5581 atcgagcatt gggacaatgc cgtcaagcag ggctaccacg cggcgctgaa catgctgggc
     5641 cgcaggatgc gctatgacga ggtgtcctat ttcttctgtg aagtggggga catgggattc
     5701 gacatgcttg gcgctgtcga ggagacggat gaatggatcg cgcgcggctc cctggaggag
     5761 cgctcgttcg cgctcttcta cctcaaggac aatgtgccgc gggcgatgtt ctcgctgggc
     5821 cgtccccccg gcgaagtccg gctctcggaa gggttgatcc gctatcggac gaatgtgctg
     5881 ccgcagaagg cgtcgctggc caatccggat ttcgcgcttg accaactggc cagccagacc
     5941 gtgctgatcc tgcagggcgg cggcgcgctc ggcgctttcg agtgcggcgt cgtcaaggcc
     6001 ctggaggagc atcggatctt tcccgacatc gtcgccggca tttcgatcgg ggcattgaac
     6061 ggggcgatca tcgccagcaa tcccggaaag tcgaccgccg tgctggaggc attctggcgc
     6121 gacctgcagg ttgcttcgtc cccggcgctg ccccagcccc tgcgccgcgc gatgacggcg
     6181 atgcagatcc tgcagttcgg cgtgccgaat ttcttcaggc ccaggtggct cccgatgctg
     6241 ggcgagccct ggacggcgcc gtgggcctgg accagtttct acgacgtttc cccgatgcgg
     6301 cggctgatag agaaatacgt ggacttcgcc gcgctcaagt ccagcccggt ccggctgctc
     6361 gtcggcgcgg tcaatgtgct gacggcggag ctgagggtgt tcgacagcta cgtcgacgat
     6421 ttgacgccgg atcacatcct ggcgagcggc agcctgccgc ccgggttccc ctggaccttc
     6481 gtcgacgggg ttccctattg ggacggcggc gtgatcagca actcgccgct cgacctggtg
     6541 gtcgaccgct gcgggcccga tggcaagcgc gttttcatcg ttgacctgtt tgcggggcaa
     6601 aaggcgctgc cggcgaatat gatggaaatc ctggcccggc gcgacgagat cgtctattcg
     6661 gagcgcgtgc gcagcgactt gcgccagcgg gagctgaagg acgcctatcg cgacctggtc
     6721 gatagcattt tgcaggatgt cgagccgggg cagcgcgaca ggatcaagct gcgtcccgcc
     6781 tatatccagc tcatgggcga tggcgcgcag atgcacatta cgcggttcgt ccgcaaggga
     6841 gcgcctgacg agccgtcgtc gcgggattac gacttttccg atgtatcgat ccagacgaat
     6901 caggcccagg gctacgagtt ggcccgcgac acgctgaacg agcgggcggc caaggttgag
     6961 gtgcgggaag atttgccggc cgacggccgg gaaacaggga agttccatcg cggaaatcct
     7021 ccgcggcgtt tcgaggagtg gagaaatggc tgaagaaaaa agcaatacga tgatgcaggt
     7081 ctatctggcg aacatgcggc tgatgcaacg gctgggcgga tcgttgcagg cggccggcat
     7141 gcaatggtcg cagcagggcc agacggccgc gacgacggcc gctgcggaat tcagcgcaga
     7201 actgggcgaa ctgctcaata ccaaggactt tcccggcttg ctcgccctgc agctcaatat
     7261 cgtgcgccgg cattgggatc agaaccaggc gctgctgctg caggcgttca acctcggcgc
     7321 gcagaatccc gaactggcgg ccgagttccg ggaggccttc ggcgaatgga agcaggaact
     7381 ggccggtatc acgagcgagg cgggtagcgt ggcccaggcc ctgacgccct ggacgcagta
     7441 cctggcccaa ttccagcagc tttggaaccc cgagggcggt ggcgccgcgg ccaagggcaa
     7501 gccgggcagg aagtgacgca ggccgtcttt cccgccggac ccgcggcggc gcggggatat
     7561 cccgtcaagt agcccctgcc gggtggccgg tgcccggggc gtggcagtgc gccccgagca
     7621 ggcggccgaa ggcgtcggca atgcgccgga tataggggtt cttgtgggcc agcggcccgt
     7681 cggggcgctc gtagtgcacc agcatggtgg cgttggcctc gaacacgaag accgcgccgt
     7741 cgggcagcag cgtgaagtcg gcgccggcat agtccaggtc catctgccgg cccagcgctt
     7801 cgagggcggc ccaggccgcc atgcccagct cggtccgcgg gtccgcgaga aagcgcgctt
     7861 cttcttcccg cttccagggt tcggccagca tgccggcgga atggtagtgg accatccagt
     7921 gcggcgagat cgccaggtga taggggtagg gccggcggtc cacgtacacg acgcggtatt
     7981 tgcggtaatg cccgtcggga ctgcggcagt cccggtaggc gctcaggtag ccggggccct
     8041 gcaggccgcg cagcgcctgg tcgaactcgg cttggtccgt gcagcgcacc aggcccgcgc
     8101 cgccatggct ggccgcggga cggaccagca gcggtagcgc cagcccggcc tcgtccagcc
     8161 gccgcgccag cgcatcgagg gcttcccgcg gcgtgttcgg cgcctcgtcc aatctcaggc
     8221 agcgcgcggt ttgcaggccg gcaatgtcgt cgagcagcgc cggcaggcgg tcgcgccggg
     8281 tggcggcgac gcgcccgggg gcgttgagca gcggcctggc atagcgctgc gcgaagccat
     8341 gcaggcgcgc ggccagcggt acggcgacat cgggctcgcc gatggcgttg aaggccaggt
     8401 cggaagaggg cagggccgcg tcctcggctg gctcggcata gtcgatggcg tacttgatgc
     8461 ggcagttgtc cggcgtgggc accagcacct cgaaggggac gttgcccgcg gcccgccccg
     8521 cgcacagcag cagcacgcgc cgcctgggct ggccggcggc ttcgatgaag acgcgctgca
     8581 ggccataggc gcggtcgcgg cactgcccgg cttgccgggc gtcgccggcc agggcgtgga
     8641 tcccggcca
//
LOCUS       DASWPO010000089         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1269739, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000089 DASWPO010000000
VERSION     DASWPO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269739"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VGC69_05260"
     CDS             complement(<1..415)
                     /locus_tag="VGC69_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY0294728.1"
                     /translation="MSARIISLRSYPIKSCAGIDLAEAELTRAGLAHDRRWMLVDAGG
                     QFLTQRQWPRMALIRTALDAGMLRLSAPGMEALDVPLDGSGLAATAEPVTVWRDTVPA
                     RAESGRANDWFSRFLAHPCRLFKVDVQSTRPARSEW"
     gene            complement(522..2834)
                     /gene="parC"
                     /locus_tag="VGC69_05265"
     CDS             complement(522..2834)
                     /gene="parC"
                     /locus_tag="VGC69_05265"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY0294729.1"
                     /translation="MTDTNQTDLFATQPNEDESIPLGRYAEQAYLDYAVSVVRGRALP
                     DVGDGQKPVQRRILYAMQAMGLATGAKPVKSARVVGDVLGKYHPHGDQAAYDAMVRMA
                     QNFSLRYPLIDGHGNFGSRDGDNAAAMRYTEARLTPFAKLLLDELDEGTVDFIPNYDG
                     SQQEPQQLPARLPVALLNGASGIAVGMATEMPSHNLGEIARACVALLRNPKLSDDELY
                     ALVPGPDFAGGGQIITPAGDIAQLYASGRGSLKARARWQFEEMARGQWQLVVHELPPG
                     ASCQKVLEEIEELTNPKIRAGKKSLSPEQQQAKAMMLNLLDAVRDESGKDAAVRLVFE
                     PKTSRIDRDEFVNTLLAQTSMESNVPVNLVCIGIDGRPRQKGLRGILAEWLDFRTDTV
                     MRRTRHRLDKVIDRIHVLEGRMVVYLNVDEVIRTIRESDEPRAALMARFKLSERQAED
                     ILEMRLRQLARLEGIKIEQELAQKREEQGRLQELLDNPSSLKRLIIKEIEADAKQYGD
                     ARRTLIETAERAVLEAKVVDEPVTVIASQKGWLRSRQGHGHDAAQFGFKQGDDLYGAF
                     ECRTTDTLIAVGDNGRVYSVAVSGLPSARGDGQPVTAMIDLEAGTRIVHMIAAAAETR
                     WLFTTRGGYGFAASLGDLVGRNKAGKQFVTLEAGDTLLRPVPLFTGADRLALLSGKGK
                     FLVFGLDEVKTQPGGGRGTILMGLDGSDGLAQVVPIGAGGLRVTGIYRNKPVEDVLAG
                     KDLAVYLGKRARKGRALDVRVKEPLLSPVL"
BASE COUNT          464 a         1052 c         1115 g          534 t
ORIGIN      
        1 cccactccga gcgggccgga cgggtggatt ggacgtcgac cttgaacagg cggcagggat
       61 gcgccagaaa gcggctgaac caatcgttgg cgcggccgct ttccgcgcgc gcgggcaccg
      121 tgtcgcgcca gaccgtcacc ggctccgcgg tggcggcaag cccggagccg tcgagcggca
      181 cgtccagggc ttccatgccg ggcgcgctca gccgcagcat gccggcgtcg agcgccgtgc
      241 ggatcaaggc catgcgcggc cattgccgct gcgtgaggaa ctgcccgccg gcatcgacca
      301 gcatccagcg ccggtcatgc gcgaggccgg cgcgcgtcaa ctcggcctcg gccagatcaa
      361 tgccagcgca agacttgatg ggataactgc gcaggctgat gatgcgagcg gacatggcat
      421 tgggctcatg tgagtcgtgg accccggaag aatccgaagc acagcccctt ttcaggggct
      481 agcatcggaa ttctgcaggg cagccacgac ggctaaagaa ctcaaagaac aggcgacagc
      541 aaaggctcct tcacccgcac atccagcgcc cgccccttgc gtgcgcgctt gccgagatag
      601 acagccaggt ccttgccggc cagcacatct tccaccggtt tattgcgata gatgcccgtc
      661 acgcgcaggc cgcccgcgcc gatgggcacc acctgcgcca ggccgtcgct gccgtcgagc
      721 cccatcagga tggtgccgcg cccgcccccg ggttgcgtct tgacctcgtc caggccgaac
      781 accaggaact tgcccttgcc cgacagcagc gccagcctgt cggcgcccgt gaacagcggc
      841 accgggcgca gcagcgtatc gccggcctcc agcgtgacga actgcttgcc ggccttgttg
      901 cggccgacga gatcgcccag gctggccgca aatccgtagc cgccgcgcgt ggtgaagagc
      961 cagcgggtct cggccgccgc cgcgatcatg tgcacgatgc gcgtgccggc ctcgagatcg
     1021 atcatggcgg tgacgggctg gccgtcgccg cgcgccgaag gcaggccgga cacggccacc
     1081 gaatacacgc ggccgttgtc gcccacggcg atgagcgtgt cggtggtgcg gcactcgaag
     1141 gcgccgtaga ggtcgtcgcc ctgcttgaag ccgaactggg cggcgtcatg gccatggccc
     1201 tggcgggagc gcagccagcc cttctgcgac gcgatcacgg taaccggctc gtcgaccacc
     1261 ttggcctcga gcaccgcgcg ctcggcggtc tcgatcagcg tgcggcgcgc gtcgccatat
     1321 tgcttggcgt cggcctcgat ctccttgatg atgaggcgct tgagcgaaga cgggttgtcc
     1381 agcagctcct gcagccggcc ctgctcttcg cgcttctgag ccagctcctg ctcgatcttg
     1441 atgccctcca ggcgcgccag ctggcgcagg cgcatctcga ggatgtcctc ggcctggcgc
     1501 tccgacagct tgaagcgggc catcaatgcc gcgcggggct cgtcggattc gcggatggtc
     1561 cggatgacct cgtcgacatt caggtagacc accatgcggc cttcgagcac atggatgcgg
     1621 tcgatgacct tgtccagcct gtgccgcgtg cggcgcatca cggtgtcggt gcggaagtcc
     1681 agccactcgg cgaggatgcc gcgcaggccc ttctggcgcg gccggccgtc gatgccgatg
     1741 cacaccaggt tgaccggcac attgctttcc atgctggtct gcgcgagcag cgtgttgacg
     1801 aattcgtcgc ggtcgattct ggaggtcttg ggctcgaaca ccaggcgcac ggcggcgtcc
     1861 ttgcccgact cgtcgcgcac ggcgtcgagc aggttgagca tcatggcctt ggcctgctgc
     1921 tgctcggggg agagactctt cttgccggcg cggatcttgg ggttggtgag ctcctcgatc
     1981 tcttccagca ccttctggca cgaagcgccg ggcggcagtt cgtgcaccac caattgccat
     2041 tggccgcgcg ccatttcctc gaactgccag cgcgcgcgcg ccttgagcga gccgcggccg
     2101 cttgcgtaga gctgcgcgat gtcaccggcc ggcgtgatga tctgcccgcc gccggcgaag
     2161 tcggggccgg gcaccagggc gtacagctcg tcgtccgata gtttgggatt gcgcaacagc
     2221 gccacgcagg cccgggcgat ctcgcccagg ttgtgcgagg gcatttcggt ggccatgccc
     2281 acggcaatgc cggacgcgcc gttcaagagc gcgacgggca gccgcgcggg cagctgctgc
     2341 ggctcctgct ggctgccgtc gtagttgggg atgaagtcga cggtgccttc gtcgagttcg
     2401 tcgagcagca gcttggcgaa aggcgtgagg cgcgcttcgg tatagcgcat agcggcggcg
     2461 ttgtcgccgt cgcgcgagcc gaagttgccg tggccgtcga tcaaggggta gcgcagggag
     2521 aagttctgcg ccatgcgcac catggcgtcg taggcggcct ggtcgccatg cggatggtat
     2581 ttgccgagca cgtcgccgac gacccgggcc gatttgacgg gcttggcgcc ggtggccagg
     2641 cccatggcct gcatggcgta caggatgcgg cgctgcacgg gcttctggcc gtcgcccacg
     2701 tccgggagcg cgcggccgcg cacgacggag acggcgtagt ccaggtaggc ctgctcggcg
     2761 tagcggccca gggggatgga ctcgtcttcg ttcggctggg tggcgaagag gtcggtttgg
     2821 ttggtgtcgg tcatgcgtgc gtcggaggtg tctgagttct tggaccgcat tgacgtattg
     2881 cgctcagcgc ccggtggggc cggcggggct tcgctgtgcc gggctcgggc ccaagatcac
     2941 gggccctcgg cccctgcggg gctgccccgt cccagaggcg ccccgccagc cccaccgggc
     3001 gctgagcgca atacgtcaat gctgcaatgt ctagaaatgg agcgctgctg tcttccgtaa
     3061 tgatgatgct atccagtcct tcgaggcttg gggctggccg ccgcgcctgg gatccggtgc
     3121 ggcgcctctg cggcggggca gccccacagg ggccgagggc ccgtg
//
LOCUS       DASWPO010000090        17346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2252054, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000090 DASWPO010000000
VERSION     DASWPO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 17346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17346
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252054"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4077
                     /locus_tag="VGC69_05270"
     CDS             <1..4077
                     /locus_tag="VGC69_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nitrate reductase/sulfite reductase
                     flavoprotein subunit alpha"
                     /protein_id="HEY0294730.1"
                     /translation="VGCGMVLQVEGGRVVKIAGDPDHPANAGRLCTKGSSAHTALHDA
                     GRLRDAYVRQARGQDPAPLPMADAIADTARRLRGIIDAHGPDAVSLYVSGQMSIEAQY
                     LANKLAKGYIGTQYIESNSRLCMASAGSGYKLSLGADAPPGSYEDFDRADLFFVIGAN
                     MADCHPILFLRMMDRVKAGARLIVVDPRRTATADKAHLFLQIRPGADLALLNGLLYLL
                     HEAGRVDPGFIARHTEGWDAMPGFLAGYAPDKVAAITGIAEADIRQAAQWIGEAPEWM
                     SCWTMGLNQSTRGTWHTNALCNLHLATGKICRPGSGPFSLTGQPNAMGGREMGYMGPG
                     LPGQRSVQVEDERRFVEAMWNLPAGTLRGEGGQGTVDMFSRMAAGEIKACWIICTNPV
                     ASLPNRANAVAGLRAAELVIAQDAFLDTETNRYADILLPGALWAEDEGVMVNSERTLT
                     LMQKAVEPPGQAMADWRIIAEVACAMGYGDAFRYGSAAEVFDEIARFSNPRTGYDLRG
                     ASHAALREGPQQWPVAPQRTARRNPIRYLNDGVSQALRTEADGSVPHLAFATPTGRAR
                     FFARPHGETAERTSPDFPMVFNPGRLQHQWHTMSKTGKVAMLNKLNPAPFVEIHPADA
                     LALGIAARDRVEVRSARGRAVLPAVVTGRVRPGNCFAPMHWGDVYGEDLCVNAVTNDA
                     VDAVSLQPEIKFSAVSLSRVETPAVAPGVGPATAVSVAARDAAAAQEAPMPSIDQFAS
                     LLGLAESAAPKLGAAESTYLSGFINGLRMSAAGLAGTVPALPANAPFPPETRLWVDGL
                     LAGLFSRAGPAQAEPAPQSARILRARPKVQLLWASQTGNAESLTERYATQLMQSGFEI
                     RMACMSDFPAANLAKAQYVLLMSSTFGDGDPPDNGQDFWADLQAAQAPHLDGVRYAVL
                     AFGDRNYDQFCGHGRHLDERLAALGASRIMDRVDCDAEYQADADKWLEHVIVRIKEED
                     AAHHAVGASGMPAAGTVPTRAQPAAARLVGNRRLNLPGAMKDTRYIALAASNLEYEAG
                     DALGVWPGNCPALVDEILASTRLAADAAVTLEGLGEMRLGDALGRHLDITRPQAQALA
                     FLARRGKHAALEALLSGERKAELRQWLWGRQLADVLHEYPAELSAAELAAMLHRLQPR
                     LYSIASSPAAHRGEVHLTVSAVRFNNGRHERKGVASTFLADRAGEADVPVFVQKSAHF
                     RLPASGDAPVIMVGPGTGIAPFRGFLHERRAAGARGRNWLFFGERSARTDFYYRDEIA
                     AMRDDGFLTRLDLAFSRDQDEKIYVQDRMRERGAELWAWLEAGAHFYVCGDASRMAKD
                     VDAALKAVVARHGGMDEEQAGEYVARLSRERRYARDVY"
     gene            4273..4629
                     /locus_tag="VGC69_05275"
     CDS             4273..4629
                     /locus_tag="VGC69_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HEY0294731.1"
                     /translation="MKPILKTAAVAALAVSLASVSMSANAWGRYHHGGGGPGWAVGAA
                     VGAVVGLAAATVAGVANTATAVVAPAPVYAPAPGYYAPAPVYAAPPPVVYAQPAYVAP
                     GYYAPVVVRPRVYVPY"
     gene            complement(4758..6062)
                     /locus_tag="VGC69_05280"
     CDS             complement(4758..6062)
                     /locus_tag="VGC69_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294732.1"
                     /translation="MQQQPTVGSHRSAWDLIRAVVRVTSGNFLEQFDFFLFGFYATQI
                     SHVFFPTGNDFTSLMSTFAVFGVGFLMRPLGAVILGAYIDRVGRRKGLITTLSLMALG
                     TILIAFVPGYETIGMLAPLLVLIGRLLQGFSAGAELGGVSVYLAEMASPGKKGIFTAW
                     QSGSQQVSIMVAALLGYALNVSMDSATIAAWGWRVPFFVGCLIIPLIFMLRRRMEETQ
                     EFAARAHQPRPSLGEVAAALLKDWDTMLAGALLVAMTTTAFYLITVYAPTFGRSVLKL
                     STSDSLIVTLFVAISNFIWLPIGGALSDRIGRKPVLVTMTVLTILTSYIALTYLAAAP
                     SFDRMLGVLLWLSFLYGIYNGAMIPALAEVMPQRVRVAGFSIAYSLATAIFGGFTPAI
                     STALIQYTNDHAAPGYWMSFSALCGLLATLWFYRKNAVHPTA"
     gene            6300..7433
                     /locus_tag="VGC69_05285"
     CDS             6300..7433
                     /locus_tag="VGC69_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY0294733.1"
                     /translation="MTQITQFPFVSVSGKPEARGRAYGQQAAGRVRKSAAMYGQTLVE
                     LGYDAMARSELVKHFAREIENFAPHYLEEMRGIAAGADVPFEDIVMVNARTEVVAKAR
                     ADKKKAAELEPGDGCTGALILPTRSAGGNLIHGQNWDWRAECAQTSIVLRIRNDDGPD
                     MLTFVEAGGLARSGLNSAGVSITANYLECERDFRQLGVPLSLIRRKVLEQQHFALAIK
                     AVATTPKSCSNNIMIGMAQGFGIDFECTTDEAFPIYPGSDDLIVHANHWVSEVALCKL
                     RDTGRASTPESQYRDWRVRRLLNEQDKLTREDLKRALFDDFGTPYSVCRPPRPGSHDN
                     ISASVAMVVMEPALGLMEVAPLPALNRAFTRYSLTAEPELLAA"
     gene            7492..9048
                     /locus_tag="VGC69_05290"
     CDS             7492..9048
                     /locus_tag="VGC69_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294734.1"
                     /translation="MKGKFLWPALALAWGLAAAGGVQASTLRISMTADIRSSEPGVNR
                     DNNSDSVVMHVVEGLVAYGEDAAVRPLLAKSVEVSPDGLSYTFTLREGVKFHNGAALT
                     SADVLWTWQHDMRAGSGWRCASEFDGHGVSKVVGVQTPDAGTVVFHLDKPNGLFLDTL
                     ARTDCGGTGILNKDSVKADGSWDKPIGTGPFMFGDWVRGQYVSLRKFADYANRGGKPD
                     GYTGSKRPLVDEVRFMVIPDEATVKAALQRGDIDIIEPLDNNDVAVLQKEPGIKVAQA
                     PVMSMTALLMQTQDPLLQNVKLRQAIAHALDYGQIAAASSDNLDTPNNSVVPRSSSYY
                     GSTQQQGWNYDPGLSKRLLAEAGYHGQELVILTNKNYPSSYDAAVVMQAMLQSAGINA
                     TLSVMEWGTQLDRYNSGKYQLMSFPYSARLDAALNYEMLTGDKTRQPRKVWDNPQAIE
                     QIRSAQTETDRAKRQALFDQLHNEFLADVPSIPLFNGLDIAAFRDNIKGYQPWSVKKP
                     RAWEVEKTGG"
     gene            complement(9050..10615)
                     /locus_tag="VGC69_05295"
     CDS             complement(9050..10615)
                     /locus_tag="VGC69_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294735.1"
                     /translation="MTTVRSPKILAPALLALALAAALPAQAAQLVVAEPADIRSTNPG
                     VNRDDTTDGVVLNIVEGLVGYRGDGTVGPLLAKSVDVSEDGLTYTFKLREGVKFHNGA
                     ALTSADVLWNWQRYMDPKTDWRCRADFDGHGDLKVTDVSAPDADTVVMKINKKSAVFL
                     DSLARTDCGMTAILQKDSVKADGSWDKPVGTGPFMFKEWKHGEYVLLSAFKDYVSPSA
                     GDKPDGYVGAKQPRVDEVKFLVVPDPSAAKAGLLSGAIDAGEIMPSDAIELKGDDKVG
                     VLPTHDATKHTLLFQTQDPLLQNVKLRQAIATSLDIPQIVEAASEGLGQANASAVSAT
                     SAYYGATQKQAYKYDPARAQQLLKEAGYKGEPIVIYANKRAHVPSYQVAVMAQAMMQA
                     VGINAQIEVLEWATQLDRYNSGKYQISSFSYSSRLDPALSYEQFAGPKAKQPRKVWDD
                     PQAQKLIDASFLESDPAKRQALFDQLHALMLEQTPLLILYNGAEAWGVGKRVQGFSVW
                     EGKPLAWDTRVAR"
     gene            complement(10628..11254)
                     /locus_tag="VGC69_05300"
     CDS             complement(10628..11254)
                     /locus_tag="VGC69_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0294736.1"
                     /translation="MSKTKTDSTGLIDRIARDIQSGTFGLGVWLKQIDLQERYGATRL
                     EIRRALDHLAAKRLVMHFPNRGYHVHTVTTERHEQIQDIRVLLETGAAADLMPNVTAA
                     KVRALRALAERFVKLLQNGTLMELYEVNLAFHAAMYDLCGNRELAALILEIRSRAPSA
                     PTSQWATRRRGRIETSAREHLDMVDALEARDIKRLQKIIAAHIRQNVS"
     gene            complement(11296..12921)
                     /locus_tag="VGC69_05305"
     CDS             complement(11296..12921)
                     /locus_tag="VGC69_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294737.1"
                     /translation="MKTLVSVSDLTLTVGQGGREIVSKVSFDVAPGEFVGLVGESGSG
                     KTQAARAIIGLTPPPLVRAGGTLLFEGQDITLAKPATLRQLRGARIGMVFQEPMTSLN
                     PSMSVGRQLEEGLALHRRKMSRAERLERIRNMLARVGIRDPKAAMQAWPHEFSGGMRQ
                     RMMLASVMLLEPALLIADEPTTALDAVVQRDVLELLVDLTREHNTAVLMISHDLPMVA
                     RYTERMVVMQHGQVLETGSTRDILARPAHPYTRKLLDAMTRRAPARPLSRETPIVEVK
                     DLVVDYAGRQRLFSRTPRYRALQGISLQVRPREVVAVVGGSGSGKTTLGRAIAGLLAP
                     TAGQILFRDRPVARHSADWNDYRLNCQMVFQDPYSSLDPRMTIGQLVGEGLRIVPGLD
                     AGAKRRRVDEVLSEVGLGADYAGRFPHELSGGQRQRVAIARAIVRRPAFVIADEPVSA
                     LDVTVRAQVLDLFADLQEKHGFSCLFISHDLGVVEQVADRVVVMKDGGIIEEGARDEV
                     FDHPKEDYTRTLLSAIPALEPTETGGVRLKWRL"
     gene            complement(12945..13772)
                     /locus_tag="VGC69_05310"
     CDS             complement(12945..13772)
                     /locus_tag="VGC69_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294738.1"
                     /translation="MRKRKRIAANALLGGVLVGLLIVAAVLGVVWTPHDPLKLNFISR
                     LKPPGGAFALGTDEFGRDELSRLMAGASISVWISLLTVSCAVVAGTLIGAIAGFVRGW
                     TDRIIMAFINALLAFPGLLLALGLLAVFGANKYGIILALSLAYTPSVTRIVRGTVLSL
                     REKEFIESSRVLGNSELYTLCRHILPNCVAPLTVLATSMFGWVILAESALSFLGLGVP
                     PPAPTWGNMLAAARPFMAQASYLSIFPGLSIALTLLGINLLGDAVRDRLDPRMRGSR"
     gene            complement(13778..14722)
                     /locus_tag="VGC69_05315"
     CDS             complement(13778..14722)
                     /locus_tag="VGC69_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294739.1"
                     /translation="MLRFALSRIAMAIPTLLIVAVAVFVMIRLIPGDPAQLMLGDLAT
                     PAALAELRARLGLDQPWSTQFGIWFTRMLHGDLGLSITTGQPVLPLVWERFLISARVV
                     VVAVILAVLVAVPAGMIAAWKQNKAPDLLLVGTATLLVSLPTFWLGLLLLILFGLKLQ
                     WLPVVGYVSLGTDWKAGALYLAMPIATLFLHEIGVMLRMSRASTLEVLRLDYITHARA
                     KGLPESTVLLRHALRNAFGPTWTLIGLVLGNLLGGIAVVETVFTLPGLGRLLVDAIFA
                     RDYPVIQGCLLFVGVVYVVVNLIVDLFYPIFDPRVAAE"
     gene            complement(14784..15641)
                     /locus_tag="VGC69_05320"
     CDS             complement(14784..15641)
                     /locus_tag="VGC69_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0294740.1"
                     /translation="MPQTTSKPIDRASALYDLGNSIIYASRRDARFSYCTYVPPHLDS
                     ARHPMEMVVIVHGTGRAFVEYRDAFAEFARWNDCIVLCPLFPAGVLGDGNRDGFKQLR
                     EGDIRYDDILLDMVDEVGERFGLQFPTFSLFGYSGGGQFVNRFALLHPEKLWAVSIGA
                     PGSVTLLDPQQDWWVGTRDVQARFGKSIDLDALRRMAVHMVVGKADLETWEITHREGG
                     KFYMPGANDAGKTRPERMETLRRSFEAAGVAVTLDLIDNMPHDGMKAVGAVQDFLAGE
                     LRKRREAGR"
     gene            15821..16738
                     /locus_tag="VGC69_05325"
     CDS             15821..16738
                     /locus_tag="VGC69_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294741.1"
                     /translation="MKAVFIDANEPMVEIARRLHQAGEFDLVVHDNPDIKPEEVPGLL
                     DGAPIVIIDHTYLPTDVARRCAGLRHVVFLGTGARSYMNPEELAELGVAVHIIKGYGD
                     TAVAECAFALMWAAARELANMDRGMRAGNWLRTEGMQLTGKTLGLVGFGGIAAEMARL
                     ALGAGMQVVAWNRTPKAHPGVAFVPLDELLAASDVVSLHLLLNDETRGMFSARRIAQM
                     RDGVILVNTARAALVDQDAMLAALASGKIRHAGIDVYEVEPVPADHPLLKVANVTLSS
                     HSAFRTPEASDNLIEAAFAHCRRIVAQGA"
     gene            complement(16749..>17346)
                     /locus_tag="VGC69_05330"
     CDS             complement(16749..>17346)
                     /locus_tag="VGC69_05330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0294742.1"
                     /translation="RYPVDIAPFVAMRDTSPESYAALRALLGPEHVGVLFTTQALTPP
                     AGLSVQISASGYQMVATRLPAQPSGRHDFVPLTAADAPAMQELVALTKPGPFAERTVE
                     LGTYLGVRSNGRLVAMAGERMRLAGQVEISAVCVHPEHRGHGYARDLILTLMHRQIAQ
                     GIVPMLHTFADNAPAIALYEQIGFAIRQTLHVTGLKRT"
BASE COUNT         2820 a         5725 c         6074 g         2727 t
ORIGIN      
        1 gtgggctgcg gcatggtgct gcaggtcgag ggcggccgtg tcgtcaagat cgcgggcgac
       61 ccggaccatc cggccaatgc cggccgcctg tgcaccaagg gctcgtccgc ccacacggcc
      121 ttgcatgacg ccggccgcct gcgggatgcc tatgtgcgcc aggcgcgcgg gcaggacccg
      181 gcgccgctgc ccatggccga cgccatcgcc gacacggccc gccgcctgcg cggcatcatc
      241 gatgcgcatg ggccggatgc cgtgtcgctg tatgtgtcgg gccagatgtc gatcgaggcc
      301 cagtacctgg ccaacaagct cgccaagggc tatatcggca cgcagtacat cgagtccaac
      361 tcgcgcctgt gcatggccag cgcgggcagc ggctacaagt tgtcgctggg cgccgacgcg
      421 ccgccgggct cctacgagga cttcgaccgc gccgatctgt tcttcgtgat cggcgccaac
      481 atggccgact gccatcccat cctgttcctg cggatgatgg atcgcgtgaa ggccggcgcc
      541 cggctcatcg tggtcgatcc gcgccgcacg gccacggcgg acaaggccca tctcttcctg
      601 cagatccggc cgggcgccga cctggccctg ctcaatggcc tgctgtattt gctgcacgag
      661 gccggccgcg tcgatcccgg tttcatcgcc cggcacaccg aaggctggga cgccatgccg
      721 ggtttcctgg ccggctacgc gccggacaag gtggcggcca tcaccggcat tgccgaggcc
      781 gatatccgcc aggccgcgca gtggatcggc gaagcgcccg agtggatgag ctgctggacc
      841 atgggcctga accagagcac gcgcggcacc tggcatacca acgcgctgtg caatctgcac
      901 ctggccaccg gcaagatctg ccggccgggc agtggccctt tctcgctgac cgggcagccc
      961 aatgccatgg gcgggcgcga gatgggctat atgggtccgg gcctgccggg ccagcgttcg
     1021 gtccaggtcg aagacgagcg ccgtttcgtc gaagccatgt ggaacctgcc cgccggcacg
     1081 ctgcgcggcg agggcgggca aggcacggtg gacatgttct cgcgcatggc cgcgggcgag
     1141 atcaaggcct gctggatcat ctgcaccaat cccgtggcga gcctgcccaa tcgcgccaat
     1201 gccgtcgccg gcctgcgggc ggccgaactc gtgatcgcgc aggatgcttt cctcgacacc
     1261 gaaaccaacc ggtatgcgga catcctgctg ccgggcgccc tgtgggccga ggacgaaggc
     1321 gtgatggtca attccgagcg cacgctcacg ctgatgcaaa aggcggtcga gccgcccggg
     1381 caggccatgg ccgattggcg gatcattgcc gaagtggcct gcgccatggg gtatggcgat
     1441 gcctttcgct acggcagcgc ggccgaggtg ttcgacgaga tcgcgcgctt ttccaacccc
     1501 aggaccggct acgacctgcg cggcgcaagc catgcggccc tgcgcgaagg gccgcagcag
     1561 tggccggtgg cgccgcagcg cacggcgcgg cgcaatccca tccgctacct caacgacggc
     1621 gtgagccagg ccttgcgcac cgaggccgac ggcagcgtgc cgcacctggc cttcgcgacg
     1681 cccaccggcc gggcccgctt ctttgcccgc ccgcacggcg agacggccga gcggacctcg
     1741 ccggactttc ccatggtctt caacccgggg cgcttgcagc accagtggca caccatgagc
     1801 aagacgggca aggtggccat gctcaacaag ctcaaccccg cgcctttcgt cgagatccat
     1861 ccggccgacg ccctggccct gggcatcgcg gcccgggacc gcgtcgaggt ccgctcggcg
     1921 cgcggccgcg ccgtgctgcc ggccgtggtg accgggcgcg tgcgcccggg caactgcttc
     1981 gcgcccatgc actggggcga tgtgtacggc gaagacctgt gcgtgaacgc cgtgaccaac
     2041 gacgcggtcg atgccgtgtc gctgcagccc gaaatcaaat tcagcgcggt ctcgctcagc
     2101 cgtgtcgaga cgcctgcggt cgcgccaggc gtcggccccg cgacggccgt atccgttgcg
     2161 gcgcgcgacg ccgcagcggc ccaggaagcc cccatgccgt ccatcgatca gttcgcctcc
     2221 ctgctcggtc ttgccgagtc ggccgcgcca aaactcggcg cagccgaaag cacctatctt
     2281 tcggggttca tcaacggctt gcgcatgagc gcggccggtc tggcgggcac ggtgccggcc
     2341 ctgccggcca atgcgccctt cccgcccgag acgcggttgt gggtcgacgg cttgctggcg
     2401 ggcctgttca gccgcgccgg gcccgcccag gcggagcccg cgccgcaaag cgcgcgcatc
     2461 ctgcgcgcgc ggcccaaggt gcagttgctg tgggcctcgc agaccggcaa tgccgagtcg
     2521 ctcaccgagc gctacgccac gcagctgatg cagtccggtt tcgagatacg catggcctgc
     2581 atgtcggact ttcccgcggc caatctggcg aaggcgcagt acgtgctgct gatgagcagc
     2641 accttcggcg acggcgatcc gccggacaac ggccaggact tctgggcgga cctgcaggcg
     2701 gcgcaggcgc cgcacctgga cggcgtgcgc tacgccgtgc tggccttcgg cgaccggaac
     2761 tacgatcaat tctgcgggca tggccgccat ctcgacgaac gcctcgccgc cctgggcgcc
     2821 agccggatca tggaccgtgt ggactgcgac gccgaatacc aggccgatgc tgacaagtgg
     2881 ctggaacatg tcatcgtccg catcaaggaa gaggatgccg cgcatcatgc ggttggcgcg
     2941 tccggcatgc ctgcggccgg gacggtgccg accagggcgc agcctgccgc ggcgcgcttg
     3001 gtgggcaacc ggcgcctgaa cctgccgggc gccatgaagg acacgcgcta tatcgccttg
     3061 gcggcctcga acctggagta cgaagccggt gacgccctgg gggtatggcc cggcaattgc
     3121 ccggcgctgg tcgacgagat cctggcatcg acccgccttg cggccgacgc ggccgtcacg
     3181 ttggaggggc tgggcgagat gcgcctgggc gacgccctgg gccggcacct ggacatcacc
     3241 cgtccgcagg cgcaggcgct tgccttcctg gcgcggcgcg gcaagcacgc tgccctcgag
     3301 gcgttgctga gcggcgagcg caaggccgag ctcaggcagt ggctatgggg ccggcagttg
     3361 gcggacgtgc tgcacgagta cccggccgaa ttgtcggcgg ccgagctggc ggccatgttg
     3421 catcgcctgc agccgcgctt gtattccatc gcttccagtc cggccgcgca tcgcggcgag
     3481 gtgcatctca cggtgtcggc cgtgcgcttc aacaacggcc ggcacgagcg caagggcgtg
     3541 gcctcgacct ttctggccga ccgcgccggc gaggcggatg tgccggtctt cgtgcagaaa
     3601 tccgcccatt tccgcctgcc cgccagcggc gacgcgcccg tcatcatggt cggcccgggc
     3661 accggcatcg cgccgttcag gggcttcctg cacgagcgcc gcgcggccgg cgcgcgcggg
     3721 cgcaactggc tctttttcgg cgagcgcagc gcgcggaccg atttttacta tcgcgacgaa
     3781 atcgcggcca tgcgggacga tggctttctt acccgcctgg acctggcctt ctcgcgcgac
     3841 caggacgaga agatctacgt gcaggaccgt atgcgcgagc gcggcgcgga gctctgggcc
     3901 tggctggaag cgggggcgca tttctatgtg tgcggcgatg cctcgcgcat ggccaaggat
     3961 gtggatgcgg cgctcaaggc cgtcgtggcc cggcatggag gcatggacga ggagcaggcc
     4021 ggcgagtatg tggcccgttt gtcgcgggag cgccgctatg cgcgcgacgt gtattgaggc
     4081 aattgtgaca aaccatgttc gcggcgaggg gcggcccgcc ccattgacac ggactgaaat
     4141 ttttgccccg ggccgccagg cttgccgtcc ggaactccca cctatactga ccgtccaaac
     4201 ctcatacatc cggaccttgc cggatttctg ccgagcagat tttccaccaa cccctcggct
     4261 taggagtgca cgatgaaacc gatcctgaaa accgccgccg ttgccgcgct tgccgtgagc
     4321 ctggcttcgg tcagcatgag tgccaatgcc tggggacgct accaccatgg cggtggcggt
     4381 ccgggttggg ccgtgggcgc cgccgtgggc gccgtggtcg gcctggccgc cgcgacggtc
     4441 gccggcgtgg ccaataccgc gacggccgtc gtggcgccgg cgccggtcta cgcgcccgcg
     4501 ccgggctatt acgcgccggc tcccgtctat gccgcgccgc cgccggtggt ctacgcccag
     4561 cccgcgtacg tggcgccggg ctactacgcg ccggtcgtcg tacgcccgcg cgtgtacgtg
     4621 ccgtattgat ccgcgcgtat tgatggtttt accgggtggc agcctgcggg cttgccatca
     4681 ggcccaaaca aaagggctgg ccttcgcggg ccagcccttt ttgttggtcg cgggccgcgg
     4741 cgcggcccgg ccggcggtca cgccgtcgga tgcacggcgt tcttgcggta gaaccacagg
     4801 gtcgccagca gtccgcacag cgcggagaag ctcatccagt agccgggggc ggcatggtcg
     4861 ttggtgtact ggatgagtgc cgtcgagatg gccggcgtga agccgccgaa gatggccgtg
     4921 gccaggctgt aggcgatcga gaagcccgcc acgcgcacgc gctgcggcat gacttcggca
     4981 agtgccggaa tcatcgcgcc gttgtagatg ccgtacagga acgacagcca cagcagcacg
     5041 ccgagcatgc ggtcgaacga gggcgcggcg gccaggtagg tcagcgcaat gtacgaagtg
     5101 aggatggtca gcaccgtcat cgtgaccagc acgggcttgc ggccgatgcg gtcgctgagc
     5161 gcgccgccga tgggcagcca gatgaagttg gagatggcca cgaacagggt cacgatcagg
     5221 ctgtcggagg tgctgagctt gagcaccgac ctgccgaagg tgggcgcgta cacggtgatc
     5281 aggtagaaag cggtggtggt catggccacc agcagggcgc ccgccagcat ggtgtcccag
     5341 tctttcagca gcgcggcggc cacttcgccc agggaagggc gcggctggtg cgcgcgagcg
     5401 gcgaattcct gggtttcttc catgcggcga cgcagcatga agatcagcgg gatgatcagg
     5461 cagcccacga agaagggcac gcgccagccc caggcggcga tcgtggccga gtccatggag
     5521 acgttgagcg catagcccag cagcgcggcg accatgatgg acacctgttg gctgcccgac
     5581 tgccaggccg tgaagatgcc tttcttgccg ggcgaggcca tttcggccag gtacaccgac
     5641 acgccgccca gttcggcgcc cgccgagaag ccttgcagca ggcggccgat gagcaccagc
     5701 agcggagcca gcatgccgat ggtttcgtag ccgggcacga aggcgatgag gatggtgccc
     5761 agcgccatca ggctcagcgt ggtgatgagg cccttgcggc ggccgaccct gtcgatgtag
     5821 gcgccgagga tcaccgcgcc caggggacgc atcagaaagc ccacgccaaa cacggcaaag
     5881 gtcgacatca gcgaggtgaa gtcgttgccg gtggggaaga agacatgcga gatctgcgtc
     5941 gcatagaaac cgaacaggaa aaagtcgaac tgttcgagga agttgccgct cgtcacgcga
     6001 accaccgcgc ggatcaggtc ccaggcagat cgatggctgc ctacggtcgg ttgctgctgc
     6061 atgtttttgt cctttgattt ggaataggtg cagaacggcg caaggttaca ccagcgctgc
     6121 ggcgcagagt gctgcatcgc aacggcttcg cgtgattcag aatattgaaa gcaagcgtgg
     6181 ggaatccgcc tttctgcatg ggtaaacccc ctctgtattt ttggcgccaa aatgttctag
     6241 cattttccgt tttccgtctt ctccctattg atgcgccggg tgccggcgca ccggactcca
     6301 tgacccagat tacgcaattc ccctttgttt ccgtttccgg caagcccgaa gcccgcggcc
     6361 gcgcctacgg ccagcaggcg gccggccgcg tgcgcaagag cgcggccatg tacggccaga
     6421 ccctggtcga gctcggctac gacgccatgg cgcgcagcga gctcgtcaag cacttcgcgc
     6481 gcgagatcga gaacttcgcg ccgcactacc tggaagagat gcgcggcatc gcggccgggg
     6541 ccgacgtgcc ctttgaagac atcgtcatgg tcaacgcccg caccgaggtc gtggccaagg
     6601 cgcgggccga caagaaaaaa gcggccgagc tggaacccgg cgacggctgc acgggcgcgc
     6661 tcatcctgcc cacgcgctcg gccggcggca acctgatcca cggccagaac tgggactggc
     6721 gcgccgaatg cgcgcagacg tccatcgtgc tgcgcatccg caacgacgac ggccccgaca
     6781 tgctgacctt cgtcgaggcg ggcggcctgg cgcgcagcgg cttaaacagc gccggggtgt
     6841 ccatcacggc caattacctg gaatgcgaac gcgacttccg ccagctgggc gtgccgctct
     6901 cgctcatccg ccgcaaggtg ctcgagcagc agcacttcgc gctggcgatc aaggccgtgg
     6961 cgacgacgcc caagtcctgc tcgaacaaca tcatgatcgg catggcccag ggcttcggca
     7021 tcgacttcga atgcaccacc gacgaggctt tccccatcta cccgggcagc gacgatctca
     7081 tcgtgcacgc caaccactgg gtcagcgaag tcgccctgtg caagctgcgc gacacgggcc
     7141 gcgccagcac gccggagagc cagtaccgcg actggcgcgt gcgccgcctg ctcaacgagc
     7201 aggacaagct cacgcgcgag gatctcaagc gcgcgctgtt cgacgatttc ggcacgccgt
     7261 actcggtgtg ccgcccgccg cgcccgggca gccacgacaa tatttccgcc tcggtggcga
     7321 tggtggtcat ggagcccgcg ctggggctga tggaagtcgc gcccctgccg gccctgaacc
     7381 gcgccttcac ccgctacagc ctgaccgcgg agcccgagct gctggctgcc tgagtcccgc
     7441 gattccgcat tcgccctgcg cgcccgcgca tacctggaga gcatgctgca tatgaaagga
     7501 aaatttctgt ggcccgcgct ggcgctggca tggggcctgg cggccgccgg cggcgtccag
     7561 gcatccaccc tgcgcatttc gatgacggcg gacatccgtt cttccgagcc cggcgtcaac
     7621 cgcgacaaca acagcgattc cgtggtgatg cacgtggtcg aaggcctggt ggcctacggt
     7681 gaagacgcgg cggtgcggcc gctgctggcc aagtcggtcg aggtcagtcc ggacggcctg
     7741 agctacacct tcacgctgcg cgagggcgtg aagttccaca acggcgccgc gctgacctcg
     7801 gccgacgtgc tgtggacctg gcagcatgac atgcgggcgg ggtcgggctg gcgctgcgcc
     7861 agcgagttcg acggccatgg cgtgtccaag gtcgtcggcg tgcaaacgcc cgatgccggc
     7921 acggtcgtgt tccacctcga caagcccaac ggcttgttcc tggacacgct ggcccgcacg
     7981 gattgcggcg gcaccgggat tctcaacaag gactcggtca aggccgacgg cagctgggac
     8041 aagcccatcg gcactggccc cttcatgttt ggcgattggg tgcgcgggca gtatgtcagc
     8101 ctgcggaaat tcgccgacta tgccaatcgg ggcggcaagc ccgacggcta caccggctcg
     8161 aagcggccgc tggtcgacga ggtgcgcttc atggtcatcc cggacgaggc cacggtcaag
     8221 gcggccttgc agcgcggcga catcgacatc atcgaaccgc tggacaacaa cgacgtggcg
     8281 gtcctgcaga aggagcccgg catcaaggtg gcgcaggcgc cggtgatgag catgacggcc
     8341 ttgctgatgc agacccagga tccgctgctg cagaacgtca agctgcgcca ggccatcgcg
     8401 catgcgctcg attacgggca gatcgccgcg gcatccagcg acaacctgga tacgcccaac
     8461 aattcggtcg tgccgcgcag ttcgtcgtac tacggcagca cccagcaaca aggctggaac
     8521 tacgaccccg gactgagcaa gcgcctgctg gccgaggccg gctaccacgg gcaggagctg
     8581 gtcatcctga ccaacaagaa ctatccctcg tcgtatgacg ccgccgtggt catgcaggcc
     8641 atgctgcagt cggcgggcat caacgccacg ctgtcggtga tggagtgggg cacgcagctc
     8701 gaccgctaca acagcggcaa gtaccagctg atgagctttc catattcggc tcggctggat
     8761 gcggcgctga actacgagat gctgaccggc gacaaaaccc ggcagccgcg caaggtgtgg
     8821 gacaaccccc aggccatcga gcagatccgg tcggcccaga ccgagaccga ccgggccaag
     8881 cgccaggccc tgttcgacca gctgcacaac gagtttctgg ccgacgtgcc gtccatcccg
     8941 ctctttaacg gcttggacat cgccgcgttc cgcgacaata tcaagggcta ccagccctgg
     9001 tcggtgaaaa agcccagggc gtgggaagtg gagaagaccg ggggttgact tatcgggcga
     9061 cgcgggtgtc ccacgccagc ggcttgcctt cccacacgga gaagccctgc acccgcttgc
     9121 ccacgcccca ggcttccgcg ccgttgtaca ggatcagcag cggcgtttgt tccagcatca
     9181 gggcatgcag ctggtcgaac agggcctggc gcttggccgg gtcggactcc aggaagctgg
     9241 cgtcgatcag cttctgggct tgcgggtcgt cccacacctt gcgcggctgc ttggccttgg
     9301 ggcccgcgaa ctgttcatag ctcagggccg gatccagccg ggacgagtac gagaacgagc
     9361 tgatctggta tttgccgctg ttgtagcggt cgagctgcgt ggcccattcc agcacctcga
     9421 tctgggcgtt gatgccgacc gcctgcatca tggcctgggc catgaccgcc acctggtagc
     9481 tcggcacgtg ggcgcgcttg ttggcgtaga tcacgatggg ctcgcccttg tagccggctt
     9541 ccttcagcaa ttgctgggcg cgggcgggat cgtacttgta ggcctgcttc tgcgtcgcgc
     9601 cgtagtaggc cgacgtggcg ctcacggccg aggcattggc ttggcccagc ccttccgagg
     9661 ccgcctcgac gatctggggg atgtccagcg aggtcgcgat ggcctggcgc agcttgacgt
     9721 tctgcagcag cgggtcctgg gtctggaaca gcagggtgtg cttggtggcg tcgtgcgtgg
     9781 gcagtacgcc gaccttgtcg tcgcccttga gctcgatggc gtcggaaggc atgatctcgc
     9841 cggcatcgat ggcgcccgag agcaggccgg ccttggcggc cgaggggtcc ggcacgacca
     9901 ggaacttgac ctcgtcgacc cgcggctgct tggcgcccac atagccgtcg ggcttgtcgc
     9961 cggccgaggg cgagacgtag tccttgaagg ccgacagcag cacgtattcg ccgtgcttcc
    10021 actccttgaa catgaagggg ccggtgccga cgggcttgtc ccagctgccg tcggccttga
    10081 ccgaatcctt ctgcaggatg gcggtcatgc cgcagtcggt gcgcgccagc gagtcgagga
    10141 atacggccga ctttttgttg atcttcatga ccacggtgtc cgcgtcgggc gcggacacgt
    10201 cggtgacctt caggtcgccg tggccgtcga aatcggcgcg gcagcgccag tcggtcttcg
    10261 ggtccatgta gcgctgccag ttccacagca cgtcggccga ggtcagcgcg gcgccgttgt
    10321 ggaacttcac gccctcgcgc agtttgaagg tgtaggtcag gccatcttcg gaaacgtcca
    10381 ccgacttggc cagcagcggc cccaccgtgc cgtcgccgcg atagcccacc aggccctcga
    10441 cgatgttgag caccacgccg tcggtcgtgt cgtcgcggtt gacgcccggg ttggtggagc
    10501 ggatgtcggc cggctcggcg acgacgagct gggcggcctg ggccggcagg gcggcggcca
    10561 gcgccaatgc caggagggcg ggggcaagga tcttgggcga gcggacggta gtcatgcggg
    10621 agactcctca ggagacgttt tggcgtatgt gggcggcgat gatcttctgc aggcgcttga
    10681 tatcgcgggc ctccagggca tcgaccatgt cgaggtgttc ccgggccgag gtttcgatgc
    10741 gcccgcgcct gcgggtggcc cattgcgaag tgggcgccga aggcgcgcgc gaacggattt
    10801 ccaggatcag ggcagccagt tcgcggttgc cgcacaggtc gtacatggcg gcatggaatg
    10861 ccaggttgac ttcgtacagt tccatcaggg tgccgttttg cagcagcttg acaaagcgct
    10921 cggccagggc gcgcagggcg cgcaccttgg cggccgtgac gttgggcatc aggtcggcgg
    10981 cggcgccggt ctcgagcagt acccgtatgt cctggatctg ctcgtgccgc tccgtggtca
    11041 cggtgtgaac gtgatagccg cggttgggaa agtgcatcac caggcgtttg gcggcgagat
    11101 ggtcgagcgc gcgccgtatt tccaggcgcg tggcgccgta gcgttcctgc aggtctatct
    11161 gcttgagcca gacgccgagg ccgaatgtgc ccgattgaat gtcgcgggca atgcggtcga
    11221 tgagtccggt cgagtcggtc ttggtcttgg acatggtggg ttcacgcggg cgtggattgt
    11281 ctgggcagcc tattgttata gacgccattt caggcgcacg ccgcctgtct cggtgggttc
    11341 cagcgcgggg atcgcggaca gcagggtgcg ggtgtagtct tccttggggt ggtcgaatac
    11401 ctcgtcgcgc gcgccttcct cgatgatgcc gccgtccttc atcaccacga cgcggtcggc
    11461 cacctgttcc accacgccca ggtcgtggct gatgaacagg cacgaaaagc cgtgcttctc
    11521 ctgcaggtcg gcgaacaggt ccagcacctg cgcgcgcacg gtcacgtcca gcgccgagac
    11581 cggctcgtcg gcgatcacga aggccggccg gcgcacgatg gcccgggcga tggccacgcg
    11641 ctggcgctgc ccgcccgaga gttcatgggg aaaacggccg gcatagtcgg cgcccaggcc
    11701 cacttcgctc agcacctcgt cgacgcggcg gcgcttggcg ccggcatcca ggccggggac
    11761 gatgcgcagg ccttcgccca ccagctgacc gatggtcatg cgcgggtcca gcgacgagta
    11821 cgggtcctgg aacaccatct ggcaattgag gcggtaatcg ttccagtcgg ccgagtgcct
    11881 ggccacggga cggtcgcgga acaggatctg ccccgcggtc ggcgccagca ggccggcgat
    11941 cgcgcggccc agcgtggtct tgcccgaacc cgagccgccc accacggcca cgacctcgcg
    12001 cggcctgacc tgcaggctga tgccctggag cgcccggtag cggggcgtgc gcgagaacag
    12061 gcgctggcgg ccggcatagt ccaccaccag gtccttgacc tcgacgatgg gtgtttcccg
    12121 cgacagcggc ctggccggcg cgcggcgcgt catggcgtcg agcagcttgc gcgtataggg
    12181 atgcgcgggc cgggccagga tgtcgcgggt cgagccggtt tccagcacct gcccatgctg
    12241 catgaccacc atgcgctcgg tgtagcgcgc caccatgggc aggtcgtggc tgatcatcag
    12301 cacggcggtg ttgtgctcgc gggtcaggtc caccagcagc tcgagcacgt cgcgctgcac
    12361 caccgcgtcc agcgcggtgg tgggctcgtc ggcgatcagc agggcgggct ccagcagcat
    12421 caccgaagcc agcatcatgc gctggcgcat gccgccggag aactcgtgcg gccaggcctg
    12481 catggcggcc ttggggtcgc ggatgcccac gcgcgccagc atgttccgga tgcgttcgag
    12541 gcgctcggcg cggctcatct tgcgccgatg cagcgccagg ccttcctcga gctgccggcc
    12601 cacgctcatg gacgggttga gcgaggtcat cggttcctgg aacaccatgc cgatgcgcgc
    12661 gccgcgcagc tggcgcagcg tcgcgggctt ggccagggta atgtcctggc cctcgaaaag
    12721 cagggtgccg ccggcgcgca ccagcggcgg cggcgtgagg ccgatgatgg cgcgcgccgc
    12781 ctgcgtcttg ccgctgccgg attcgccgac caggccgacg aattcgcccg gggcgacgtc
    12841 gaaggacacc ttgctgacga tctcgcggcc gccctggccg acggtcaggg tgaggtccga
    12901 tacggagacc aatgtcttca tgttgcccgc ttggcccggc gcattcatcg cgacccccgc
    12961 atgcgaggat cgaggcggtc gcgcacggcg tcgcccagca ggttgatgcc gagcagggtc
    13021 agcgcgatcg acaggccggg aaagatcgac agataggatg cctgggccat gaagggccgc
    13081 gcggcggcca gcatattgcc ccaggtgggc gccggcggcg gcacgcccag ccccaggaag
    13141 gacagggcgc tctcggccag gatgacccag ccgaacatcg aggtggccag cacggtcagg
    13201 ggcgccacgc aattgggcag gatgtggcgg cacagggtgt agagctcgga attgcccagc
    13261 acgcgcgagg attcgatgaa ctccttttcc cgcagcgaca gcacggtgcc gcgcacgatg
    13321 cgggtgaccg agggcgtata ggccaggctc agcgccagga tgatgccgta cttgttggcg
    13381 ccgaacacgg ccaagaggcc gagcgccagc agcaggccgg gaaaggccag cagcgcattg
    13441 atgaacgcca tgatgatgcg gtcggtccag ccgcgcacga agccggcgat cgcgccgatc
    13501 agggtgccgg cgacgacggc gcagctcacg gtgagcaggc tgatccagac gctgatcgag
    13561 gcgccggcca tgagccggga cagctcgtcg cggccgaatt cgtcggtgcc cagggcgaag
    13621 gcgccgccag gcggcttgag gcgggaaatg aagttgagct tgagcgggtc gtgcggcgtc
    13681 cagaccacgc ccaggacggc ggcgacgatc aggaggccga ccagcacgcc gcccagcaag
    13741 gcattggccg cgatgcgttt tcttttccgc aagtttttca ttccgcggcc actctcggat
    13801 cgaagatggg gtagaagagg tcgacgatca ggttcaccac cacgtagacc acgcccacga
    13861 acagcaggca gccctggatg accgggtagt cgcgcgcgaa gatggcgtcg accagcagcc
    13921 ggcccaggcc gggcagggtg aagacggtct cgaccacggc gatgccgccc agcaggttgc
    13981 ccagcaccag gccgatcagc gtccaggtgg ggccgaaggc attgcgcagg gcgtggcgca
    14041 gcagcacggt ggattcgggc aggcccttgg cgcgggcgtg cgtgatgtag tccaggcgca
    14101 gcacttccag cgtgctggcg cgcgacatgc gcagcatcac gccgatctcg tgcaggaaca
    14161 gggtggcgat gggcatggcc agatacaggg cgcccgcttt ccagtccgtg cccagcgaaa
    14221 catagcccac caccggcagc cattgcagct tcaggccgaa caggatcaag agcagcaggc
    14281 ccagccagaa ggtgggcagc gacaccagca gcgtggccgt gccgaccagc agcaggtcgg
    14341 gcgccttgtt ctgcttccac gcggcgatca tgccggccgg cacggccacc agcacggcca
    14401 ggatgacggc cacgaccacc acgcgggcgc tgatcaggaa gcgttcccat accagcggca
    14461 gcacgggctg gccggtggtg atggagaggc ccaggtcgcc atgcagcatg cgggtgaacc
    14521 agatgccgaa ctgggtgctc cagggctggt ccaggcccag ccgggcgcgc agctcggcca
    14581 gggcggcggg cgtggcgagg tcgcccagca tcagctgggc cgggtcgccg gggatgaggc
    14641 ggatcatcac gaagaccgcc acggccacga tgagcagcgt ggggatggcc atggcgatgc
    14701 gggacagggc aaagcgcagc atggccgctc ctactcggtg ccggcggcgg cttgccgcgc
    14761 cggacgcggg acggatgggc gcatcagcgc ccggcctcgc gtcgcttgcg cagttcgccc
    14821 gccagaaagt cctggacggc gccgacggct ttcatgccgt cgtgcggcat gttgtcgatc
    14881 aggtccagcg tgaccgcgac accggccgcc tcgaaagagc ggcgcagcgt ctccatccgt
    14941 tcgggccggg tcttgccggc gtcgttggcg ccgggcatgt agaacttgcc gccttcgcgg
    15001 tgggtgattt cccaggtctc gagatcggcc ttgcccacca ccatgtgcac ggccatccgg
    15061 cgcagcgcgt ccaggtcgat ggacttgccg aagcgggcct gcacatcgcg cgtgcccacc
    15121 caccagtcct gctgcgggtc gagcagcgtc accgaacccg gcgcgccgat ggacacggcc
    15181 cacagctttt ccgggtgcag cagggcgaag cggttgacga actggccgcc gccggaatag
    15241 ccgaacagcg agaacgtcgg gaattgcagg ccgaatcgct cgcccacttc gtccaccatg
    15301 tcgagcagga tgtcgtcgta gcggatgtcg ccctcgcgca gctgcttgaa gccgtcgcgg
    15361 ttgccgtcgc ccagcacgcc ggccgggaac agggggcaca gcacgatgca gtcgttccag
    15421 cgggcgaact cggcgaaggc gtcgcggtac tcgacgaagg cgcggccggt gccgtgcacg
    15481 atcacgacca tctccatggg gtggcgggcg ctgtcgaggt ggggcggcac gtaggtgcag
    15541 taagagaacc gggcgtcgcg gcgcgaggcg tagatgatgc tgttgcccag gtcgtagagc
    15601 gcgctggcgc ggtcgatggg cttggaagtg gtctggggca tggctgatgt ccggggtaca
    15661 ggcaggggcc tgggaaaaat cttcacctaa attggcgcca attttaagaa ggcggcgcgc
    15721 ctggcgacag ccggggaatc ccctagacga ccgtccggca cttgatgccg gcggcgttgt
    15781 gtcttctact agtgtccccc gtccgcaacg tatccattcc atgaaagccg tattcatcga
    15841 cgccaacgaa cccatggtcg aaatcgcccg gcgcctgcac caggctggcg agttcgatct
    15901 ggtcgtccac gacaatcccg acatcaagcc cgaggaggtg cccggcctgc tcgacggcgc
    15961 gcccatcgtc atcatcgacc acacctacct gcccaccgac gtggccaggc gctgcgcggg
    16021 cctcaggcac gtcgtgttcc tgggcacggg cgcgcgctcg tacatgaacc ccgaggagct
    16081 ggccgagctg ggtgtcgccg tgcacatcat caagggctat ggcgacacgg ccgtggccga
    16141 atgcgccttc gcgctgatgt gggcggcggc ccgcgagctg gccaatatgg accgcggcat
    16201 gcgggccggc aattggctgc gcaccgaggg catgcagctc accggcaaga cgctgggcct
    16261 ggtgggcttc ggcggcatcg ccgccgagat ggcccgcctg gcgctcggcg cgggcatgca
    16321 ggtcgtggcc tggaaccgca cgcccaaggc gcatcccggc gtcgccttcg tcccgctgga
    16381 cgaactgctg gccgcgagcg atgtggtgtc gctgcacctg ctgctcaatg acgagacgcg
    16441 cggcatgttc tcggcccggc gcatcgcgca gatgcgcgac ggcgtcatcc tggtgaacac
    16501 ggcgcgcgcc gcgctggtcg accaggacgc catgctggcg gcgctggcca gcggcaagat
    16561 ccgccatgcc ggcatcgacg tctacgaggt cgagcccgtg ccggccgacc accccctgct
    16621 caaggtggcg aacgtcaccc tgtcgtcgca ctcggccttc cgcacgccgg aagccagcga
    16681 caacctgatc gaggcggcct tcgcgcactg ccgccgcatc gtggcgcaag gcgcctaggc
    16741 ggcgccgttc aggtgcgctt cagtccggtg acgtgcagcg tctgccggat ggcgaagccg
    16801 atctgctcgt acagggcgat ggccggcgcg ttgtcggcga aggtgtgcag catgggcacg
    16861 atgccctgcg cgatctggcg gtgcatcagc gtcaggatca ggtcgcgcgc atagccgtgg
    16921 ccgcggtgtt cgggatgcac gcagaccgcg ctgatctcca cctggccggc cagccgcatg
    16981 cgttcgccgg ccatggccac gagccggccg ttcgagcgca cgcccagata ggtgcccagt
    17041 tccacggtgc gctcggcaaa cgggccgggc ttggtgaggg cgaccagctc ctgcatggcc
    17101 ggggcgtccg cggccgtgag cggtacgaag tcgtgcctgc ccgaaggctg ggcgggcagg
    17161 cgcgtggcca ccatctggta gccgctggcc gagatctgga cggacaggcc ggcaggcggg
    17221 gtcagggctt gggtcgtgaa caatacgccg acgtgctcgg gccccagcag cgcgcgcagc
    17281 gcggcgtagg attcggggga agtgtcccgc atggcgacga aaggcgcgat gtcgaccgga
    17341 tagcgc
//
LOCUS       DASWPO010000091         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2876714, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000091 DASWPO010000000
VERSION     DASWPO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876714"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VGC69_05335"
     CDS             <1..431
                     /locus_tag="VGC69_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEY0294743.1"
                     /translation="WLHRPQHAATLAGPEAKLAAKVLPLAQAGVYDPPWVRDIARVLG
                     EPEERVRQLLRKLLRRGEVAQVVKDLFYHREHIDALAALVATLCAQKGSVQAADFRDV
                     VGLGRKRSIQILEFFDRAGYTRRLRDTHVLRSDGTAFRDK"
     gene            490..585
                     /locus_tag="VGC69_05340"
     tRNA            490..585
                     /locus_tag="VGC69_05340"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:524..526,aa:Sec,seq:tca)
     gene            complement(591..1151)
                     /locus_tag="VGC69_05345"
     CDS             complement(591..1151)
                     /locus_tag="VGC69_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0294744.1"
                     /translation="MSAPRRRLLRAGLAAALAVPAARALAAPAQGPTDAALKAATGPN
                     WATWKGGPAPSLDLPDLEGHTRGLDEFLGLVVIVNFWATWCEPCQEEIPALSALADSR
                     ADDGLRLLAVNNAESREKIDAFLDHLQIQGLVLQDRNGAAVRDWKVVGMPANFVVDRD
                     GKIRLWHLGALDWTKPAVLDPVTALL"
     gene            complement(1148..2251)
                     /locus_tag="VGC69_05350"
     CDS             complement(1148..2251)
                     /locus_tag="VGC69_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY0294745.1"
                     /translation="MDRRNFLRLSSAAGALYAGGLGLNRCADAAPVTAGWRSFELATE
                     VQVRDSGKQAQIWVPVPFAQDTAYQRGAQTTWNIEGNGTAQLVRAPGYDVQMLAVQWP
                     DAQDAPRRIVVTSRFQTRNRRVDLDQPAPGFKPASADALRRYLQPTALLPTDGIVKAT
                     SDRITRGHRGDLARARAIYEWVADNTCRTASTRGCGTGDVRYMLTANDLNGKCADINA
                     LFVALARAAGIPARDAYGIRVADSELGYKSLGKSGDVTKAQHCRAEFYAAGYGWVPVD
                     PADVRKVMLEEPPGDLPASDAQVRNARVMLFGAWEMNWVAYNHGHDVALPGAQQPAVP
                     FLMYPNGETRDGRLDSLDPDAFRYRISARPLSA"
     gene            2384..>3435
                     /gene="selD"
                     /locus_tag="VGC69_05355"
     CDS             2384..>3435
                     /gene="selD"
                     /locus_tag="VGC69_05355"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY0294746.1"
                     /translation="MTLATVAPGPRLTSLSHGGGCGCKIAPGVLSALLARSFAAGPSQ
                     GKHAPPGGQQAEGAAWGQHPSPMFKPCADLLVGNETADDAAVYRLNDEQALIATTDFF
                     MPIVDDPYDFGRIAATNALSDVYAMGGTPILALAIVGMPINVLPHEVIAEVLRGGESV
                     CAEAGIPLAGGHSIDSVEPIYGLAAMGLVHPSRVKRNADARAGDVLILGKPLGVGILS
                     AALKKNLLDEAGYRAMIEATTMLNRPGAALSGLPGVHAITDVTGFGLLGHTLEMARGA
                     GLAAHLRLAGLPWLEQVQAYAAQGVVTGASGRNWDSYGESVRLGAGVDDTARALLTDP
                     QTSGGLLVACAPQDAAR"
BASE COUNT          482 a         1181 c         1251 g          521 t
ORIGIN      
        1 cctggctgca tcggccccag cacgccgcca cgctggccgg cccggaggcc aagctggccg
       61 cgaaggtgct gcccctggcc caggccggcg tctacgaccc gccctgggtg cgcgacatcg
      121 cccgcgtcct gggcgagccc gaggagcgcg tgcgccagct cctgcgcaag ctcctgcgcc
      181 gcggcgaggt ggcccaggtg gtcaaggacc tgttctatca ccgggaacat atcgacgccc
      241 tggcggcgct ggtggcgacg ctgtgcgcgc aaaaaggcag cgtgcaggcg gccgacttcc
      301 gcgatgtcgt gggactgggc cgcaagcggt cgatccagat cctggagttc ttcgaccggg
      361 cgggctacac gcgcaggctg cgcgacacgc atgtgctgcg cagcgacggc acggcctttc
      421 gcgacaagta ggcaagccgc gggacgcgca tttacccgct tctttacccg ctatcattag
      481 ggccgccacg gaaggcattc gtgcccggcg gcacggctgg gcttcaaacc cagttggggg
      541 cgtcagacgt tcccaggtcg gttcgactcc ggctgccttc cgccatcgcg ctagagcagc
      601 gccgtcaccg ggtcgaggac ggccggcttg gtccagtcga gtgcgcccag gtgccacagc
      661 cgtatcttgc catcccggtc caccacgaag ttggccggca tgcctacgac cttccaatcg
      721 cgcacggcgg cgccgttgcg atcctgcagc accagcccct ggatctgcag gtggtcgagg
      781 aaggcgtcga tcttctcgcg cgactcggca ttgttgacgg ccagcaggcg caggccatcg
      841 tcggcgcggc tatcggccag ggccgacagg gccgggatct cttcctggca gggttcgcac
      901 caagtggccc agaaattcac gatcaccacc aggcccagga attcgtccag gcctcgggtg
      961 tggccttcga ggtcgggcag atcgagcgag ggcgccgggc cgcctttcca ggtggcccag
     1021 ttggggccgg tggccgcctt gagcgcggca tcggtcggcc cctgcgcggg agcggcgagg
     1081 gcgcgggcgg cgggcacggc cagggccgcg gccaggccgg cccgcagcag gcggcgtctg
     1141 ggcgcgctca cgctgacagc ggccgggcgc tgatccggta gcggaaggcg tccggatcga
     1201 gactgtcgag gcggccatcg cgggtttcgc cgttggggta catcaggaag ggcacggcgg
     1261 gctgctgcgc gcccggcagc gcgacgtcgt ggccgtggtt gtaggccacc cagttcatct
     1321 cccaggcgcc gaacagcatg acgcgggcgt tgcgcacctg ggcgtcggag gcgggcaggt
     1381 cgcccggcgg ctcttcgagc atgaccttgc gcacgtcggc cgggtccacc ggcacccagc
     1441 cgtagccggc cgcatagaac tccgcgcggc agtgctgggc cttggtgacg tcgcccgact
     1501 tgcccaggct tttgtagccc agctcggagt cggccacgcg gatgccgtag gcatcgcgcg
     1561 cggggatgcc cgcggcgcgc gccagcgcca cgaacagcgc gttgatgtcg gcgcatttgc
     1621 cgttgagatc attagcggtg agcatgtagc gcacgtcgcc ggtgccgcag ccgcgcgtgg
     1681 aggccgtgcg gcaggtattg tcggccaccc attcgtagat ggcgcgcgcg cgtgccaggt
     1741 cgcctcgatg gccgcgcgtg atgcggtcgg acgtggcctt cacgatgccg tcggtgggca
     1801 gcagcgcggt gggctgcaga tagcggcgca gcgcgtcggc gctggcgggc ttgaagccgg
     1861 gggcgggctg gtcgaggtcg acgcggcggt tgcgggtctg gaagcggctg gtcaccacga
     1921 tgcggcgcgg cgcgtcctgc gcatcgggcc attgcacggc cagcatctgc acgtcgtagc
     1981 cgggcgcccg caccagttgc gccgtgccgt tgccttcgat gttccaggtg gtctgtgcgc
     2041 cgcgctggta ggcggtgtcc tgcgcgaagg gcacgggcac ccagatctgc gcctgcttgc
     2101 ccgagtcgcg cacctgcact tcggtggcga gttcaaagct gcgccagccg gccgtcaccg
     2161 gcgcggcgtc ggcgcagcgg ttcaagccca ggccgccggc atacagcgcc ccggcggcag
     2221 aagaaagacg gaggaaattt cggcggtcca tggcaactcc cacgcacggt gaagaaggtt
     2281 gaggaacagg gccattgcat attgcatcgc ggcaatgccg caccgtcgcg gcccgggctc
     2341 ggggagtaat attctatcgg gacatatcag ataggtgaat gccatgactc ttgccaccgt
     2401 cgccccgggg ccgcgcctga cctcgctctc gcacggcggc ggctgcggct gcaagatcgc
     2461 gcccggcgtg ctgtccgcct tgctggcgcg ctcgtttgcc gccgggccgt cccaaggcaa
     2521 acatgccccc ccgggggggc agcaagccga aggcgcggcg tgggggcaac atccttcgcc
     2581 gatgttcaag ccctgcgccg acctgctggt gggcaacgag acggccgacg acgcggccgt
     2641 ctaccgcttg aacgacgagc aggcgctgat cgccaccacc gatttcttca tgcccatcgt
     2701 cgacgatccc tacgacttcg gccgcatcgc cgcgaccaac gcgctgtcgg acgtctacgc
     2761 catgggcggc acgcccatcc tggcgctggc catcgtgggc atgcccatca acgtgctgcc
     2821 ccacgaggtc atcgccgagg tgctgcgcgg gggcgagtcg gtgtgcgcgg aggccggcat
     2881 cccgttggcg ggcggccact ccatcgattc ggtcgagccc atctacggcc tggccgccat
     2941 gggcctggtg catcccagcc gcgtcaagcg caacgccgac gcgcgcgcgg gcgacgtgct
     3001 catcctgggc aagccgctgg gcgtgggcat cctgtcggcc gcgctcaaga aaaacctgct
     3061 cgacgaagcc ggctatcgcg ccatgatcga ggccaccacg atgctcaacc ggccgggcgc
     3121 ggcgctgtcc gggctgccgg gcgtgcatgc catcaccgac gtcacgggct tcggcttgct
     3181 gggccatacg ctggagatgg cgcgcggcgc gggcctggcc gcgcatctgc gcctggccgg
     3241 gctgccctgg ctggagcagg tgcaggccta tgccgcgcaa ggcgtcgtga ccggcgcctc
     3301 gggacgcaac tgggattcgt acggcgagtc ggtgcgcctg ggcgcggggg tggacgacac
     3361 cgcccgcgcg ctgctcaccg atccgcagac ctccggcggc ctgctggtgg cctgcgcccc
     3421 gcaggatgcg gcccg
//
LOCUS       DASWPO010000092        18839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2877124, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000092 DASWPO010000000
VERSION     DASWPO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 18839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18839
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877124"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VGC69_05360"
     CDS             complement(<1..244)
                     /locus_tag="VGC69_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294747.1"
                     /translation="MSLTHTGALNAPEFERAAPLRGRKKAASEDSGKVFAGRILFAVA
                     ILVIWEVSARWLLNPFWIGQPSTVAVRLWEMAVSGDL"
     gene            complement(255..1073)
                     /locus_tag="VGC69_05365"
     CDS             complement(255..1073)
                     /locus_tag="VGC69_05365"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY0294748.1"
                     /translation="MTVEFLVDDAWVKRHLDDPGVVFLDTRSAADYWQGHLPGARHFD
                     ASLLSHYDTSEAGLALLANQYATLFSLLGLGGGEHVVVYEDRSDSRAARAAWLLEYLG
                     HESVSILDGGLANLQNIALTPIAQPYAPRRFVARAQAQAVVGADELRSRLGQAGLRVL
                     DTRRPAEYYGEEKRARRNGAIPGAVHRNYVDNIGADGRFKPAAELRAAYEALGFRPDD
                     EIVAYCGGGARAAHSYYALKLAGFRTPRNYTGSWGEWGNRDDLPVEVPVRPAQG"
     gene            1329..2192
                     /locus_tag="VGC69_05370"
     CDS             1329..2192
                     /locus_tag="VGC69_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0294749.1"
                     /translation="MSTVLEEAEAATTHTPSSLDIRPWRPALGAVIRGLHYDGRLPAE
                     TRTQLRNALREYGALVFEPGTVTPQNFTELLSEFGEVSLYAGPKTPTVQGNSLANVVD
                     SSNKRQARNFLWHIDQAFRSNPPRLTALYGHDVPAFGGDTLFSNAILAYERLDPKFAA
                     YLETLTAVHYWDATGHIADRFDDPEEAGRQRALNAPIETPIVRVHPETGRKQLFVNES
                     YTTYIKNVSRTTSQNLLGILFEAIKEPEVAARYTWVSGSAILWDNRVVQHRGVGDFPG
                     QQRIFYRACVL"
     gene            complement(2208..2558)
                     /locus_tag="VGC69_05375"
     CDS             complement(2208..2558)
                     /locus_tag="VGC69_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0294750.1"
                     /translation="MEERQKTALIKAIGKTIAAKRDVAGLSQEAVAEALGISREAVSR
                     IETGVTVPSIVRLAELAEIMDCEMEALIVDASSRLRDQARHIAELLDDLEQDQRDIIV
                     SVVKQIASGFKARP"
     gene            2711..3085
                     /locus_tag="VGC69_05380"
     CDS             2711..3085
                     /locus_tag="VGC69_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEY0294751.1"
                     /translation="MDQQSKKSRRLAYAAQQSAIDKEISRLKKKAELLRIRRRKPIIA
                     QIVGAMLNFDISPQDIAEAYRTGKDAKAANGKPKPGAGKLPPKYRHPETGETWSGRGK
                     TPRWLREAESAGIAREAFFLTS"
     gene            complement(3074..5458)
                     /locus_tag="VGC69_05385"
     CDS             complement(3074..5458)
                     /locus_tag="VGC69_05385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HEY0294752.1"
                     /translation="MDSQRGFILTRHWRDTDAGTEVEFWLATDAGPRLLRLPGQTSVA
                     FIPAEQRDLAEPLLRGERGAELRPLALQDFRHRPVLGLYCPHHRQLLRLEKRLRQAGV
                     DIYEADIRPPERYLMERFITAPVLFDGTAGKDDVLREARMKPDESYRPVLKLASLDIE
                     TSMAGDLYSIALEGCGQRQVYMLGAAQPIEGELDFSLEYRASHTELLQALNDWMARHD
                     PDAIIGWNLVQFDLRILQEHAKRLGVPLRLGRGGAELEWREHSERQRHYFADAAGRLI
                     IDGIEALRSATWSFPSFSLEAVAQTLLGEGKAIDNPYDRMDAINRMFAGDKPALARYN
                     LQDCVLVTRIFEKTGLIDFLLERATVTGLPADRSGGSVAAFAHLYMPRMHRLGFVAPN
                     LGERQPESSPGGFVMDSRPGLYESVLVLDYKSLYPAIIRTFLIDPVGLVEGLNAADES
                     ATVAGYRGARFSRARHCLPDIVAQVWQGREAAKRARNAPLSQALKIIMNSFYGVLGSS
                     GCRFFDPRLASSITLRGHEIMRRTRALIEERGYEVIYGDTDSTFVWLGRAHDEAAAGA
                     IGRELAAHINQWWRAHLHRTADLESALELQFETHYQRFLMPTVRGAELGSKKRYAGQV
                     LRADGRAELVFKGMETVRTDWSPLAQQFQQALYQRVFDRQPYRDYVRDIVHRTLSGQL
                     DDLLVYRKRLRRQLGEYQRNVPPHVRAARMADEFNDQQGRPRQYQNGGWISYVITEAG
                     PEPLEAQRFPIDYGHYVDKQLRPIADAILPFLGEDFDGVVSRQLDLFERQGSGR"
     gene            5583..6509
                     /locus_tag="VGC69_05390"
     CDS             5583..6509
                     /locus_tag="VGC69_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEY0294753.1"
                     /translation="MPEFQRQPIIEILAGGSRLRFAPQAGGRLLSWDIDGQPVIHWPE
                     PADWSQPARIRGGNPLLFPFLGRHRVDGVLGRWRDAQGVVRDMPMHGFARNLPFAAQL
                     APDGKGVRMTLEDSEATRAGYPFRFRFEAAYRLVDARTLEVELATMNTGDAPLPYYAG
                     HHFYFSLPHAQRGATTLELPATGLRGQQPDGSITAAVPGKPRYRLDDPAIDDRMHCLQ
                     GTPDRPVRLLMPGQGRVIEIDLRRPGSVPWYAVTTWTEAADSDFYCVEPWLGLPDAIH
                     NGLGLRWLAPGQAETAALRITVGRLAGQEAPA"
     gene            6742..7677
                     /gene="ppk2"
                     /locus_tag="VGC69_05395"
     CDS             6742..7677
                     /gene="ppk2"
                     /locus_tag="VGC69_05395"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY0294754.1"
                     /translation="MSDSPKPSEDSQKLSEHTRRLYAEMIDSLDEELEMELDDDRLDD
                     LIEGVPAADADMDRRQYFRELLRLQGELVKLQDWVVDKKLKIVVLFEGRDAAGKGGAI
                     KRITQRLNPRVCRVAALPAPNDRERTQWYFQRYASHLPAGGEIVLFDRSWYNRAGVER
                     VMGFCNDDEYEEFFRSVPEFEKMLVRSGIILLKYWFSIQDEEQERRFLARVHDPLKQW
                     KLSPMDLESRRRWEAYTKAKEEMMERTHIPEARWWIVEGGDKKRARLNCIHHFLEQVP
                     YGEVSHPDIVLPERVHHPEYIRHPQPPEMYVPEVY"
     gene            complement(7842..8891)
                     /locus_tag="VGC69_05400"
     CDS             complement(7842..8891)
                     /locus_tag="VGC69_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HEY0294755.1"
                     /translation="MKYRKLGRTGLEVSLIGLGTMTWGEQNTEAEAHEQLDYALSRGV
                     NLIDAAEMYPVPPKAETQGRTEAYIGTWIAKRKKRDDFYLASKVAGPVRDPKRPGHIR
                     DGKTFLDRKNITAALNDSLKRLQTDYLDLYQLHWPDRTTNTFGQLTYPWVDDEHTVPV
                     EETLAVLQDLQKAGKIRHVGLSNETPWGLAQFIKQSEAKDLPRVASIQNPYSLVNRIY
                     EIGLSEFSHREDVGLLAYSPLAMGVLSGKYLNGVRPAKARLTLFERFTRYSNPQTDAA
                     AAEYAALAKQHGLSPATLALAWVNTRKFVASNLIGATTLEQLKENIDSVDVELDADVL
                     AGIEAIHVKYPNPAP"
     gene            9003..10115
                     /locus_tag="VGC69_05405"
     CDS             9003..10115
                     /locus_tag="VGC69_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY0294756.1"
                     /translation="MPTLFDPLVVGDLKLANRIVMAPLTRNRSPKAVPQAITASYYSQ
                     RATAGLLITEATAISHQGQGYADVPGLYGADQLEGWKRVTSEVHKAGGRIVVQLWHVG
                     RISHTSLQPNGGAPVAPSAITAKSKTYVLNADGSGEFLPTSAPRALGIEEIPGIVSDY
                     RKAARAAIDAGFDGVELHAANGYLIDQFLRSGTNHRTDAYGGSIENRARLMVEVLDAI
                     TAEIGAGRTGIRISPVTPANDASDPNPQPLFAYVVEKLAKYGLAYVHVIEGATGGARD
                     YQQGDTPFDYAALKSAYGKAGGKAAWMVNNGYDGALAEQAVESGAADLVAFGKPFISN
                     PDLVRRLREKAPLNEWDPSTFYGGGEKGYLDYPTLG"
     gene            10167..10243
                     /locus_tag="VGC69_05410"
     tRNA            10167..10243
                     /locus_tag="VGC69_05410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10201..10203,aa:Arg,seq:acg)
     gene            10594..11478
                     /locus_tag="VGC69_05415"
     CDS             10594..11478
                     /locus_tag="VGC69_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294757.1"
                     /translation="MNLNKLEIYCKVVEEGSVTAAAEHFHVTQPVISSHLRNLETFFG
                     AKLLYVEKRRMLLTEAGRVVYDYALSIRHATASTRSFASALAAGDAGRVVIGASYGPG
                     GYLLPLQLSRFRAAHPRVQIELAVVGSSKVIEGLADGLYDIGIVTSDDVPSEFDTELL
                     SNEPLALIAAPGHPLARRRVIDTAMLQQAEFVFPYSPRTQVWVKKRLARLGIKEPLVS
                     MKLGSWEATKHAAMAGAGLILAYRFCVEVELRSKHLKALPVQIDPLTEPVILIRSPRR
                     RYTTMQEQLVAFLHSAMN"
     gene            complement(11549..12547)
                     /locus_tag="VGC69_05420"
     CDS             complement(11549..12547)
                     /locus_tag="VGC69_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294758.1"
                     /translation="MTLLFSHFPRLPRGILAALLPVALAVAAPQAKAEFPEQPLTLVV
                     PFSPGGGTDNTARALGEGMSANLGKPIIVENRAGAGSIIGTNYVAHSKPDGYTMLLAT
                     FAHAVNPALYPNLPYDTDKAFEPVAMIAHSPNVLVVSPKLPFHSVQDLLAYAKAHPGT
                     LNYGSTGIGTSAHLAGELLKNLAGINMKHVPYKGAAPALTDLISGQIQVMLITAASAT
                     SYVKSGQVRALAVTSAQRSPAFPDLPTLAEAGVPGYQAESWSGIFVPAGTPEPVVRRL
                     RDAVAAAVKTAAFQRQVTTEGLMVDVGEPKQLSDYVSAEEARWRRVVHDAHIQALN"
     gene            complement(12774..13961)
                     /locus_tag="VGC69_05425"
     CDS             complement(12774..13961)
                     /locus_tag="VGC69_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0294759.1"
                     /translation="MKIVNVEVLLISEKLQKPWVMGLGTATKRDEIIVRVHTDEGIVG
                     IGAAYHGHSPYVIRELIQSKLAPLVIGQNPLQIQSLWERMFFGTIHLGSAAAQAIAGI
                     DIALWDIMGKATGQPVYRLLGGGDGAADRGPRLATYVGCQTLGISEDLKALADEACGY
                     VEQGFKAVKVRGGAGVKRDLAAIAAVRDAVGPDIEIMIDANATYSWPEAVRLAKGLED
                     YNTFWLEDPFDFTVGYHKDEMGKLRGLSRTPIASGGNVFTRFDFRLLAEKGGVDFLTP
                     DVVKSAGISESMKIASIASAFDIVIATHTYNGLTQIANLHFAAAIPAHIRGHVEWDPG
                     THNTFRDALVVPAAHVGHGYVEVPHGPGLGVELDKDVLAHLSFIEGPEIIGIPRRRSW
                     AVA"
     gene            complement(14558..15553)
                     /locus_tag="VGC69_05430"
     CDS             complement(14558..15553)
                     /locus_tag="VGC69_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0294760.1"
                     /translation="MQFAKFSRTGLSVSRLCLGTATFGKQTDETTSHAILDKAADAGV
                     NFIDTADMYPMGADFSLAGRSEEITGRWLKGKRGRFIVATKAGEPMGPSAWDKGSSRK
                     HLLDAIDASLRRLGTDYVDLFQLHLDDSATPLDETVEALDSIVRSGKARYVGVSNFLA
                     YRLARAIGRQDALKLARFVSVQPRYNLLFREIERELLPLAQEENLAVTPYNPLAGGLL
                     TGKYRLEENPEKGRFSEEVGRFGLRYQTRYWHTREFETLEKLKSISSELGATLPKLSI
                     AWILAHPAITSVILGASSPDQLNETLAAADYVFDAGLKSAFDETTHEYRLGDSNR"
     gene            complement(15643..16947)
                     /locus_tag="VGC69_05435"
     CDS             complement(15643..16947)
                     /locus_tag="VGC69_05435"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0294761.1"
                     /translation="MLNRENNEKLCRVGPSTPMGRALRRYWHPVARSARLERDGAPLR
                     VRLLGENFVAFRATDGRVGFFDENCPHRRASLSLARNEGNGLRCLYHGWKFDVSGKVV
                     EVPTELPERRAAFAEKVPLQHYAVREAGGIVWVCIDKEGLRTPFPNFEFVDFGPGQID
                     ARSAVIRCNWLQLLESVLDSAHLSFLHSGVLVTRPTSESPLSEGMLANMAATTAMSSP
                     TFEILERPYGFREGAIRDYGDGRRFAKIREFVAPYFSFLPGFPGKPTRRILVMSVPID
                     DTHCTQWVVNYRTDAPFEPGEVEDFWRFANPDPDNFLTPTSGPADIWGQDRQAMQAGH
                     FSGFPNRHFFEEDVVVQESMGPIVDRTREYLSQSDKTILYVRRQLLKAAEAAQRGEAP
                     WGLGAAADIDYSRIRSCAVFLDQDENWPDIDSHRLEPRHAKA"
     gene            complement(17066..18061)
                     /locus_tag="VGC69_05440"
     CDS             complement(17066..18061)
                     /locus_tag="VGC69_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294762.1"
                     /translation="MKLLSFRMSSRLLATATALGALSLGPAAQAQSSYPSKPIRLVVP
                     FPPGGAVDMLGRLVGQHIGQQMNQSVIVENRGGANGRIGDVYAAHAAPDGYTLLFGAN
                     GLATNPSLYPNDSFSEIKTLTPVAYLGSSPLIMVVPQDSPAKSLKDIIDAAKADPSKI
                     AFASAGNGSSAHLGSEMLKSVAKVSMLHVPYKGGAPAIIDLTAGRVSFMLLDPPQAMP
                     QIRGNKLRPLVVGSNTRLPLLPDVPSAADAGYPDFEATVWWGFMAPKGTPPEIVDKLN
                     SEINTALRSPDVQKVLGDLGVITKPGTPAEFGQFIQSQYSKWNALIKQAGITAEN"
     gene            complement(18092..>18839)
                     /locus_tag="VGC69_05445"
     CDS             complement(18092..>18839)
                     /locus_tag="VGC69_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0294763.1"
                     /translation="REEPLQDEEFLATMDAAGVQRAIIVPPSPVGDSNLTALESAARH
                     PDRLAIMGRFNPDTGDARERLARWLAQPGMLGIRMTFHKPQWAGWLEPGALDWYWSDC
                     ERLGIPLMLFVPGHVDKISAIAERHPGLTIVLDHMARRSDLRDDACFADLDHLLALAR
                     FPNVHVKASAVPCYSTQPYPFANLKPYLRRIYESFGPRRTMWGSDYTRIPCSYGECVD
                     HFLKELDFLDAESLEWVMGKAAAQALRWPA"
BASE COUNT         3309 a         5837 c         6249 g         3444 t
ORIGIN      
        1 agagatcgcc ggagacggcc atctcccata gccgtacggc gacggtggaa ggctggccga
       61 tccagaacgg attgagcagc cagcgcgcgg atacttccca gatgacgagg atggccacgg
      121 caaacaggat gcggccggcg aagaccttgc cgctgtcctc ggatgcggcc ttcttgcggc
      181 cgcgcagcgg ggccgctctc tcgaactcgg gggcgttcaa ggcgccagta tgggtcagcg
      241 acatggaaac tccttcagcc ttgcgccggg cgaaccggga cttccacggg caggtcgtcg
      301 cgattgcccc actcgcccca cgagccggtg tagttgcgcg gcgtcctgaa gcccgcgagc
      361 ttcaatgcgt agtacgagtg cgccgcgcgc gcgccgccgc cgcaataggc gacgatttcg
      421 tcgtcgggtc tgaagccgag cgcctcatag gcggcgcgca gttcggccgc cggtttgaag
      481 cggccgtcgg cgccgatgtt gtcgacgtaa ttgcgatgga cggcgcccgg gatggcgccg
      541 ttgcgtcgcg cgcgcttttc ctcgccgtag tattcggccg gccgccgcgt atcgagaacg
      601 cgcaggccag cctgccccag gcgcgaccgc aattcgtcgg cgccgacgac ggcctgggct
      661 tgcgcacgcg caacgaagcg gcgcggtgca tagggctgcg cgattggcgt cagcgcgatg
      721 ttttgcaggt tcgccaagcc gccgtcgagg atggacaccg attcatggcc caggtattcg
      781 agcagccagg cggcgcgcgc cgcgcgtgaa tcggagcggt cttcatagac gacgacgtgt
      841 tcgccgccgc ccagccccag cagcgagaac aaggtggcgt actggttggc cagcagcgcc
      901 aggcctgcct cggaggtatc gtaatgggat agcagcgatg cgtcgaaatg ccgggccccg
      961 ggcaggtggc cctgccagta gtcggcggcc gaacgtgtgt cgagaaacac gacgccggga
     1021 tcatccaggt ggcgcttcac ccatgcgtca tcgaccaaaa actctacagt cattgcaaag
     1081 acctctccaa gctgagtcct gcaatctacg acgcgtggca tggcgcggcc aataccgata
     1141 actcatttgt ttatccgatt tgcgccacct tccctgcgga tactcatcgt gcaagctata
     1201 aggaaataga caagcaatct ttccattgcg cgagccatgt cgataggatg tcagatccgg
     1261 cttgttcgat gatgcgtcgc aagcctaaag ccctgctcat taccttgcct ttgctggaga
     1321 gaatcgccat gtccaccgtc ttggaagaag ccgaagccgc cacaacccat acgccttcat
     1381 ctctggacat ccggccgtgg cggcctgcgc tgggcgccgt cattcgcggc ctgcactatg
     1441 acggccgtct ccccgccgaa acgcgcaccc agctcaggaa cgcgctacgg gaatatggcg
     1501 cgctggtctt cgaaccgggc accgtcacac cgcagaactt caccgaattg ttgtcggaat
     1561 tcggcgaagt gtcgctttac gccggcccca agacgccgac tgtccaaggc aactcgctgg
     1621 ccaacgtggt cgattcgagc aacaagcggc aggcgcgcaa cttcctgtgg cacatcgacc
     1681 aggccttccg ttccaacccg ccgcgcctga ccgcgctgta cgggcacgac gtgcccgctt
     1741 tcggcggcga caccctgttc agcaacgcca tactggccta tgaacggctc gacccgaaat
     1801 tcgccgctta cctggaaacg ctgaccgcgg ttcactactg ggacgccacc ggccacatcg
     1861 ccgaccgctt cgacgacccc gaggaggccg gccgccagcg cgcgctgaac gcgccgatcg
     1921 aaacccccat cgttcgcgtg catccggaaa ccggccgcaa gcaattgttc gtcaacgagt
     1981 cctacaccac ctacatcaag aacgtctcgc gcaccacgag ccagaacctg ctcggcatcc
     2041 tgttcgaagc catcaaggaa cccgaggtcg cggcgcgcta cacctgggta tcgggctcgg
     2101 ccatcctgtg ggacaaccgc gtggtgcagc accgcggcgt cggtgatttc cccggccagc
     2161 agcggatctt ctaccgcgcc tgcgtgcttt gagcatcggc ggcggcgcta gggcctggcc
     2221 ttgaagccgc tggcgatttg cttgaccacg gacacgatga tgtcgcgctg gtcttgttcc
     2281 aggtcgtcca acagttccgc aatgtgccgc gcttgatcgc gcaggcggct cgaggcgtcg
     2341 acaatcaggg cctccatctc gcaatccatg atttccgcca gctcggccag gcgcacgatg
     2401 gatggcacgg tcacgcccgt ttcaattctc gagacggctt cccggctgat gcccagcgcc
     2461 tccgccacgg cttcctgcga aaggcccgcc acgtcgcgct tggcggcgat ggtctttcct
     2521 atggccttga tcagggctgt tttctggcgt tcttccatct gcggtgtcga cctgtttgcg
     2581 cacgagcgta atggtcgagg tcgcacggaa gaattcacat gacttgaaaa gtcgttatgt
     2641 gacgtttaag atcacaagat gatttcatct tggtggaaat cggtgcaaga accggaaacg
     2701 cacgaatccc atggatcagc aaagcaaaaa atcccgacgg cttgcctatg ccgcgcagca
     2761 gtcggcaatc gataaagaaa taagccggct gaagaaaaaa gccgaattgt tgcggatcag
     2821 gcggcgcaag cccatcattg cccagatcgt tggcgcgatg ctcaatttcg atatctcgcc
     2881 ccaggacatc gccgaggcct accggacggg caaggacgcc aaggcggcaa atggcaagcc
     2941 gaaacccggg gcgggcaagc tgccgccgaa ataccggcac cccgaaaccg gggaaacctg
     3001 gagcggccgg ggcaagacac cccgctggct gcgcgaggcg gagagtgccg gcatcgcgcg
     3061 cgaggccttc tttttaacgt cctgagcctt ggcgctcgaa caagtcgagt tgccggctca
     3121 ccaccccatc gaaatcctcg cccagaaacg gcaggatggc gtcggcgatc ggccgcaact
     3181 gcttgtccac gtaatgcccg taatcgatcg ggaagcgctg cgcttccagg ggctcgggcc
     3241 cggcttcggt gatcacgtag ctgatccagc cgccgttctg gtattggcgc ggccggccct
     3301 gctggtcgtt gaattcgtcg gccatgcggg cggcgcgcac atgcggcggg acgttgcgct
     3361 ggtattcgcc cagttggcgg cgcaggcgct tgcggtagac cagcaggtca tccagttggc
     3421 ctgacagcgt gcggtgcacg atgtcgcgca cataatcgcg atagggctgg cggtcgaaga
     3481 cgcgttggta gagcgcctgc tggaattgct gcgcgagcgg cgaccagtcg gtgcgcacgg
     3541 tttccatgcc cttgaagacg agctcggccc ggccatcggc gcgcagcacc tggccggcgt
     3601 agcgcttctt gctgcccagt tccgcgccgc gcacggtggg catgagaaag cgctggtaat
     3661 gggtttcgaa ctgcagctcg agcgcgcttt ccaggtccgc ggttcgatgc agatgcgctc
     3721 gccaccactg gttgatgtgg gcggcgagct cgcggccgat ggcgccggcg gctgcttcgt
     3781 cgtgggcgcg gcccagccag acgaaggtgg agtcggtgtc gccgtagatc acctcgtatc
     3841 cgcgctcctc gatcagggcg cgcgtgcggc gcatgatctc gtggccgcgc agggtgatgg
     3901 acgaggccag ccgcgggtcg aagaagcggc agccgctgga gcccagcacg ccgtaaaacg
     3961 agttcatgat gatcttgagc gcctgcgaga gcggcgcgtt gcgcgcccgc ttggcggctt
     4021 cgcggccctg ccagacctgg gcgacgatgt cgggcaggca atgccgcgcg cgcgagaagc
     4081 gcgcgccgcg atagccggcc acggtggccg actcgtccgc ggcattcagg ccctcgacca
     4141 ggccgacggg atcgatgagg aaagtgcgga tgatggcggg atagaggctc ttgtagtcga
     4201 gcaccagcac ggactcgtac aggccggggc gcgagtccat gacgaagccg cctgggctgg
     4261 attcgggctg gcgctcgccg agattggggg ccacgaaacc cagccggtgc atgcgcggca
     4321 tgtacaggtg cgcgaaggcg gccaccgagc cgccgctgcg gtcggccggc aagccggtga
     4381 cggtagcgcg ttccagcaag aagtcgatca acccggtctt ctcgaagatg cgcgtgacca
     4441 gcacgcagtc ctgcaggttg tagcgggcca gcgcgggctt gtccccagcg aacatgcggt
     4501 tgatcgcgtc catgcggtcg tagggattgt cgatggcctt gccctcgccc agcagggttt
     4561 gcgccacggc ctccaggctg aaggagggga aactccaggt ggccgaacgc agggcctcga
     4621 tgccgtcgat gatgagccgg cccgccgcgt cggcgaagta gtggcgctgg cgttcgctgt
     4681 gttcacgcca ttccagttcg gcgccgccgc ggcccagccg cagcggcacg cccaggcgct
     4741 tcgcgtgctc ctgcaggatg cgcaggtcga attgcacgag attccagccg atgatggcgt
     4801 cgggatcgtg gcgcgccatc cagtcgttga gggcttgcag cagctcggtg tgcgatgcgc
     4861 ggtattccag ggaaaaatcg agctcgccct cgatgggctg cgcggccccc agcatataga
     4921 cctggcgttg gccgcaacct tcgagcgcga tcgaatacag gtcgccggcc atgctggttt
     4981 cgatgtcgag cgaggccagc ttgagcacgg gccggtagct ttcgtccggt ttcatgcgcg
     5041 cttcgcgcag tacatcgtcc ttgcccgccg tgccgtcgaa gaggaccggc gcggtgatga
     5101 agcgttccat caggtagcgc tccggcgggc ggatgtcggc ttcgtagatg tccacgccgg
     5161 cttggcgcag gcgtttttcc agcctgagca gctggcggtg gtgggggcag tacaggccca
     5221 ggacggggcg gtggcggaag tcctgcagcg ccagcggacg caactcggcg ccgcgctcgc
     5281 cgcgcagcag gggttcggcc agatcgcgct gctcggccgg gatgaaggcg accgaagttt
     5341 ggccgggcag gcgcaacagg cgcggcccgg cgtcggtcgc cagccagaat tccacctccg
     5401 tgccggcgtc cgtatcgcgc cagtgccggg tgaggatgaa gccccgctga gaatccacgc
     5461 tgcctgccca ggaaaaccga tgtctatcgg cgtcattcta gcgaaggccg cgggacgcgg
     5521 ctggtcgagg tctccatggt taaatccagg tccggtcggt atttgttttc acggggcgag
     5581 tcatgcctga attccagcgg caacctatca tcgaaatcct cgcaggcggc tcgcggctgc
     5641 gcttcgcccc gcaagccggc gggcgcctgc tgtcctggga catcgacggc cagccggtga
     5701 tccattggcc ggagccggcc gactggagcc agcccgcccg catacgcggc ggcaatcccc
     5761 tgttgtttcc cttcctgggc aggcaccgcg tcgatggcgt gctgggccgc tggcgcgatg
     5821 cgcagggcgt cgtgcgcgac atgcccatgc atggtttcgc gcgcaatctg ccgtttgccg
     5881 cgcagcttgc gccagatggc aagggcgtgc gcatgacgct ggaggacagc gaggccaccc
     5941 gtgccggtta tcccttccgg tttcgttttg aggcggcata ccggctggtg gatgcccgca
     6001 cgctcgaggt cgaactggcc acgatgaata ccggcgatgc gccgctgccg tactatgcgg
     6061 gccatcattt ctatttctcg ctgccgcacg cgcagcgcgg cgccaccacg ctggaactgc
     6121 cggccaccgg gctgcgcggc cagcagcccg acggcagcat cacggcagcc gtgccgggca
     6181 agccgcgata ccggctggac gaccctgcca tcgacgatcg catgcactgc ctgcagggga
     6241 cgccggaccg cccagtgcgc ctgctgatgc ccggtcaggg ccgcgtcatc gagatcgacc
     6301 tgcggcgtcc gggctcggtg ccctggtatg ccgtcaccac ctggaccgag gcggccgatt
     6361 ccgatttcta ttgcgtggag ccctggctgg gcctgccgga cgccatccac aacggcctgg
     6421 gcctgcgctg gctggcgccg gggcaggccg agacggcggc gttgcggatc acggtgggcc
     6481 ggctggccgg gcaggaagcc cccgcctgaa gcccaatgcc cctcgggcct ggcgcggccc
     6541 ggtacggtcc ctgggatgac aaagtgtttc gcgatctgtc cggggatatc ctgcaaaacg
     6601 atccgccagg cgagaacacg gcgtggcgat cgctgaggtt tcaccgtgct gtcattctgg
     6661 tgaggctaaa tggcgggcac catttgcctg tttcggccgg caagccggtc caagctgaat
     6721 ccctgatgcg agagcgaccc gatgagcgac tcccccaaac cctccgaaga ctcccagaaa
     6781 ctctccgaac acacccgccg cctctatgcg gaaatgatcg acagcctcga cgaagagctt
     6841 gaaatggagc tcgacgacga caggctggac gacctgatcg aaggcgtgcc ggccgcggat
     6901 gccgacatgg accgccgcca gtacttccgc gagctgctgc gcctgcaggg cgaactggtc
     6961 aagctgcagg actgggtggt ggacaagaag ctcaagatcg tcgtgctctt cgaggggcgc
     7021 gacgcggccg gcaagggcgg cgccatcaag cgcatcaccc agcgcctgaa cccgcgcgtg
     7081 tgccgagtgg cggccctgcc ggcgcccaac gaccgcgaac gcacccagtg gtacttccag
     7141 cgttatgcat cgcacctgcc ggcgggcggc gagatcgtgc tgttcgaccg cagctggtac
     7201 aaccgcgccg gggtcgagcg cgtcatgggt ttctgcaacg acgacgaata cgaagagttc
     7261 tttcgcagcg tgcccgagtt cgagaagatg ctggtgcgct cgggcatcat cctgctgaaa
     7321 tactggttct cgatccagga cgaggagcag gagcgccgct tcctggcgcg cgtgcacgat
     7381 ccgctcaagc agtggaagct ctcgcccatg gacctggaat cgcgccgccg ctgggaggcc
     7441 tacaccaagg ccaaggaaga gatgatggag cgcacgcaca tccccgaggc caggtggtgg
     7501 atcgtcgagg gcggggacaa gaagcgcgcg cgcttgaact gcatccatca cttcctggag
     7561 caggtgccct atggcgaagt gagccacccc gacatcgtgc tgcccgagcg ggtgcatcat
     7621 cccgaataca tccgccatcc gcaaccgccg gagatgtacg tccccgaggt gtattgatga
     7681 gaaaggccat tcaaaactga atggcctttt tcgtggcatc gatgtcaaag caagccgagg
     7741 accccggagg aatccgaagc gcagccccca ccaggggcga gcatcggaat tccgcagggc
     7801 agcctcggcg ccttaagaac tcaaaaaaga acccccaaaa atcaaggcgc cgggttcgga
     7861 tacttgacgt ggatggcctc gatgcccgcc agtacgtcgg catcgagttc cacatcgacg
     7921 ctgtcgatgt tttccttgag ctgttcgagc gtggtcgcgc cgatcaggtt gctggccacg
     7981 aacttgcggg tgttcaccca ggccagcgcc agcgtcgccg gcgacaggcc gtgctgcttg
     8041 gccagcgccg cgtactcggc cgcggccgcg tcggtctgcg ggttgctgta gcgggtgaag
     8101 cgttcgaaca gcgtcaggcg cgccttggcc ggacgcacgc cgttcaggta cttgcccgac
     8161 agcacgccca tggccagcgg cgagtaggcc aggaggccca cgtcctcgcg gtggctgaat
     8221 tccgacaggc cgatttcgta gatgcggttg accaggctgt agggattctg gatggacgcc
     8281 acgcgcggca ggtccttggc ctcggactgc ttgatgaact gcgccaggcc ccagggcgtt
     8341 tcgttggaca ggccgacgtg gcggatcttg ccggctttct gcagatcctg cagcacggcc
     8401 agggtttcct cgacgggcac ggtgtgctcg tcgtcgaccc aggggtaggt cagctggccg
     8461 aaggtgttgg tggtgcggtc gggccagtgc agctggtaga ggtcgagata gtcggtctgc
     8521 aggcgcttga ggctgtcgtt gagcgcggcg gtgatgttct tgcggtcgag gaaggtcttg
     8581 ccgtcgcgga tgtggccggg gcgcttgggg tcgcgcaccg ggccggcgac cttgctggcc
     8641 aggtagaagt cgtcgcgctt cttgcgcttg gcgatccagg tgccgatgta ggcttcggtg
     8701 cggccctggg tttcggcctt gggcggcacg gggtacattt cggcggcgtc gatcaggttg
     8761 acgccgcgcg acagggcgta gtccagctgc tcgtgggctt cggcctcggt gttctgttcg
     8821 ccccaggtca tagtgcccag gccgatcagg ctgacttcga ggccggtgcg gccaagtttg
     8881 cggtatttca cgacatactc cgggtgctga aagggttttg aagaataggg ttttgggcaa
     8941 tggtactacc ggtccagggg tatagtgcaa tgcaccattc cccgatttgc aggagtcctt
     9001 gcatgcctac attgttcgat cccttggttg tcggcgacct gaaactggcc aaccgcatcg
     9061 tcatggcgcc gctcactcgc aaccgctcgc ccaaggcggt gccccaagcc atcacggcca
     9121 gctattacag ccagcgcgcc acggccggcc tgctcatcac cgaggccacg gccatctcgc
     9181 accaggggca gggctatgcc gacgtgccgg gcttgtacgg cgccgaccag ctcgaaggct
     9241 ggaagcgcgt gacgagcgaa gtgcacaagg cgggcggacg catcgtggtg cagctctggc
     9301 acgtgggccg catctcgcac acctcgctgc agcccaatgg cggcgcgccg gtggcgccct
     9361 cggccatcac ggccaagtcc aagacctacg tcctcaacgc ggacggcagc ggcgagtttt
     9421 tgccgacctc cgcgccgcgc gcgctgggca tcgaagaaat tcccggcatc gtgtccgact
     9481 atcgcaaggc ggctcgtgcc gccatcgacg cgggcttcga cggcgtcgag ctgcatgccg
     9541 ccaacggcta cctcatcgac cagttcctgc gttcgggcac caaccatcgc accgacgcct
     9601 acggcggctc gatcgagaat cgcgcgcgcc tgatggtcga ggtgctcgat gccatcacgg
     9661 cggagatcgg cgcgggccgc accggcatcc gcatctcgcc ggtgacgccg gccaacgatg
     9721 ccagcgatcc gaatccgcag ccgctctttg cctacgtggt ggaaaagctg gccaagtatg
     9781 gcctggccta tgtgcacgtc atcgaaggcg ccacgggcgg cgcgcgcgac taccagcaag
     9841 gcgacacgcc cttcgactac gcggccctga agtcggccta cggcaaggcg ggcggcaagg
     9901 cggcctggat ggtcaacaac ggctacgacg gcgcgctggc cgagcaagcc gtggaaagcg
     9961 gcgcggccga cctggtggcc ttcggcaagc ccttcatctc caaccccgac ctggtgcgcc
    10021 gcctgcgtga gaaggcgccc ttgaacgagt gggacccatc cacgttctac ggcggcggcg
    10081 agaagggcta tctcgactac cccacgctgg gctgatccgt ccccggcacg agttcgaaat
    10141 ttctgctaga atcctgcttc tttttagcgc ccgtagctca gatggataga gtacttggct
    10201 acgaaccaag gggtcgtggg ttcaattcct gccgggcgca ccagaatatt cgttgataag
    10261 cagtgacttg gagcggcttg aagggtgaac cttcaagccg cttttctttt gccgggggac
    10321 tcttggggga ctcgctccac ccatgcgcgt cctcgatggt gacgggccgc tcgcaaggcg
    10381 gggcgatctc gatgaggctg cgggcgaatg gggttttgcc cgagcccgcc ttgccggcaa
    10441 tgacgatgtt ccgatgcagc gggacaaact tgcggaatgt ggattggcaa agcggctatc
    10501 atgccgctgg agccatgtcg cgcacgatga ttgatttcga tggtgaatat tcttagaaaa
    10561 aatggccatt ttggccaatc gatcgagatt tgaatgaatc tcaacaaact cgaaatctac
    10621 tgcaaagttg tcgaggaagg atccgtaaca gctgctgccg agcatttcca cgtgacccag
    10681 ccggtcatca gcagtcacct gcgcaatctc gagaccttct tcggtgccaa gctgctgtat
    10741 gtcgagaagc gccgcatgct cttgacggag gctggccgcg tcgtttacga ctatgcgctg
    10801 tccatccgcc acgcgaccgc gtcaacccgc agcttcgcct ccgcgctggc cgccggcgat
    10861 gcgggccgtg tcgtgatcgg cgcaagctat ggtcctggcg gctatctgct gccgcttcag
    10921 ctctcgcgct ttcgcgccgc gcatcctcgc gtgcagatcg agttggcggt ggtcggttcg
    10981 tcgaaggtga tcgaaggcct ggccgatggc ctttacgata ttggcatcgt cacatcggat
    11041 gacgttccct ccgaattcga taccgaactg ctttcgaatg agccgctagc gctgatcgca
    11101 gcgccaggcc atccgctcgc cagacggcgc gtaatcgaca cggccatgct gcagcaggct
    11161 gagttcgtgt tcccttactc gccgcgtacc caggtatggg tcaagaagcg cctggcgcgc
    11221 ctcggcatca aagagcccct ggtgtcgatg aagctggggt cctgggaagc gaccaagcat
    11281 gcggccatgg cgggcgctgg cctcatcctt gcctatcgct tctgcgtcga ggtcgaactg
    11341 cgcagcaagc acttgaaagc actgccggtg cagatcgatc ccttgaccga acccgtcatc
    11401 ctgatccgtt cgccgcgtcg gcgctatacg acgatgcagg agcaactcgt tgcgttcctg
    11461 cacagcgcga tgaattgaat cttttcaggt tcaattcccc gtcgtttgcg gcgggggggg
    11521 tgattggctt gcctagacga tccaggcatc aattcagcgc ctggatgtgg gcgtcatgca
    11581 cgacgcggcg ccagcgcgct tcctcggccg atacatagtc cgacaattgc tttggctcgc
    11641 ccacgtcgac catcaggcct tcggtggtca cctgcctctg aaaggcggcc gttttcacgg
    11701 cggcggccac cgcgtcgcgc agacgccgca ccaccggttc aggcgtaccg gcgggcacga
    11761 atataccgga ccagctttcc gcctggtatc caggcacgcc cgcttcggcc aaggtcggca
    11821 gatccgggaa ggcgggcgaa cgctgcgccg aggttacggc cagggcgcgc acctggccgc
    11881 tcttgacgta ggacgttgcg ctggctgcgg tgatcagcat gacctggatc tggccgctga
    11941 tcagatccgt cagggcgggg gcggcgccct tgtaaggtac gtgcttcata ttgatgcccg
    12001 ccagattctt gagcagttcg ccggcaagat gggctgaggt gccaatgccg gtcgaaccat
    12061 aatttagcgt gcccggatgc gctttcgcgt aggccagcaa atcctggacg ctatgaaagg
    12121 gcagcttggg gctgactacc agcacattgg gcgaatgcgc gatcatcgcg acgggttcga
    12181 acgccttgtc ggtgtcatag ggcaggttcg ggtaaagcgc gggattgacg gcatgcgcga
    12241 aggtcgccag cagcatggtg tagccgtcag gcttgctgtg cgccacgtag ttggttccga
    12301 tgatcgaacc tgcgccggca cggttttcca cgatgatggg cttgccgaga ttggcgctca
    12361 tgccttcgcc cagggcgcgg gcggtattgt ccgtgccccc gccaggggag aagggcacga
    12421 ccagggtgag gggttgttcg ggaaactcgg ccttggcctg gggcgcggcc acggccaggg
    12481 cgaccggaag cagggcggcc aggatgccgc gcggcaagcg ggggaaatgc gagaagagca
    12541 gggtcattgg tgtctccggt ttttttgtgg gattccatcg tgtgcgtgga atcgccttgc
    12601 atagtgagcc gccgccgtct ttttggggac ggtcggcgct cttagagagg ggcctcttat
    12661 ggatcgggcc ctgtaaaaac gctgaatctg gcggcactca ggctgttttc gcagtccgag
    12721 cccgagtgcg caggaagacg cgcattggac ggcctaatcg acaggcccgc tcttcaggcc
    12781 accgcccagc tgcgacgacg cgggatgccg atgatctccg gcccctcgat aaagctgaga
    12841 tgagcgagca catccttgtc cagctcgacg cccagtcccg ggccgtgcgg cacttcgacg
    12901 tagccatggc cgacatgcgc ggcgggtact acaagcgcat cgcggaacgt gttgtgcgtg
    12961 cccggatccc attcgacgtg cccgcggatg tgcgcgggaa tcgcggctgc aaagtgcagg
    13021 ttggcaatct gcgtgagtcc gttgtaggta tgtgtcgcga tcacgatgtc gaacgcgctg
    13081 gcgatcgacg cgatcttcat gctttcggaa atgccggccg acttcacgac atccggcgtg
    13141 aggaagtcga cgcccccttt ctctgccagc aagcggaaat cgaagcgcgt gaacacattg
    13201 ccgccggacg cgatcggcgt gcgggacagg ccgcgcagct tgcccatttc gtccttgtgg
    13261 tagccgacgg tgaaatcgaa cgggtcttcg agccagaacg tgttgtagtc ttccaggcct
    13321 tttgcaagcc gaaccgcttc gggccagcta taggtggcgt ttgcgtcgat catgatctcg
    13381 atgtcgggtc cgaccgcatc gcgcacggcg gcaatcgcgg ccagatcgcg cttgacgccg
    13441 gcgccgccgc ggaccttgac cgccttgaag ccctgctcga cgtagccgca tgcctcgtcg
    13501 gccagcgcct tgaggtcttc gctaatgccg agtgtttggc aacccacgta ggtggcaagg
    13561 cgcgggccgc gatccgctgc gccgtcgccg ccgccgagca gccggtatac cggctgcccc
    13621 gtggctttgc ccatgatgtc ccagagcgca atgtcgatgc cggcaatagc ctgcgcggcg
    13681 gcggaaccca aatggatcgt gccgaagaac atgcgctccc acagcgactg gatctgcagc
    13741 ggattctgcc cgatgacgag cggcgcgagc ttggactgaa tcagctcgcg gatcacatag
    13801 ggagaatggc cgtggtaagc ggcgccgatc ccgacgatgc cttcatcggt atgcacccgc
    13861 acgatgattt cgtcgcgctt ggtcgctgtg ccaagcccca tcacccaggg tttttgcagt
    13921 ttctctgaaa taagcaaaac ctcgacgttc acgattttca tgctgtgtct cctgttgccg
    13981 tattcctggc gttgaaaatt tttactcgcc gtcaccgaac aagacggccg cgataatcga
    14041 tatgcgaacc ggatgagtcc atggatacgc aatcgacata cctgatgtgt tcggcaaaat
    14101 gcgatgatcc atgcgcacaa gcgatatcac ttctggcggt aaaaatttaa acccctgcga
    14161 ttgccattga ccaattcatt ttcgtgaatc gaacgattgg aaggaggcta tgcggccgca
    14221 tggcgggaaa cgctacaagg gcatcgaaat gtggatcgac atttgccgtt gtgagggagt
    14281 tgccagcgtt gattgaaacg gctcaaggcg ttttccctcg agccgttttg tcttgggctg
    14341 gagagcgccg gcatgtcccc cggccgctga tcagggccgg gaaccggcgc gtcccgactc
    14401 ggtattccgt atcggcggcg aggagttcgt atgcctcgtc gacgttgcaa cggataagca
    14461 ggccttcgtg atcgggcagc gggtctggga gacgttcgag cgcgaggcga gccgggtcaa
    14521 cggtattccc gtcgcggcgg gatgaaaaag cgcatgacta ccgattcgag tcgcccagac
    14581 ggtattcgtg tgttgtttcg tcgaaggcag atttcaggcc ggcatcgaaa acataatccg
    14641 ccgcggcaag cgtttcgttc agctgatccg ggctgctggc cccgaggatg accgaggtta
    14701 tcgctggatg ggccagtatc caggcgatcg aaagcttggg cagggttgcg ccgagttcgc
    14761 tcgagatcga cttcagtttc tcgagggtct cgaattcacg cgtgtgccaa taccgggttt
    14821 ggtatctcaa gccgaatcgg ccaacttcct cggagaaacg tcctttttcc gggttttctt
    14881 caagacggta cttgcccgtg agaagaccgc ccgcgagcgg attgtagggc gtcaccgcca
    14941 ggttttcttc ctgggccagc ggcagcagtt cgcgctcgat ttccctgaac agcaggttat
    15001 agcggggctg taccgaaacg aatctcgcca gcttcaaggc gtcctggcgg ccaatcgctc
    15061 ttgccaggcg ataggcgaga aagttcgaga cccccacata gcgcgccttg ccggatcgga
    15121 cgatcgaatc gagcgcctcg acggtttcgt cgagcggcgt tgcggaatcg tccaggtgaa
    15181 gctggaacag atcgacgtaa tccgtgccca ggcgtctgag cgacgcatcg atcgcgtcga
    15241 gcaaatgctt gcgtgaagag cccttgtccc aggcggatgg acccatcggt tcgcccgcct
    15301 tggtcgcaac gatgaaacga ccacgcttgc ctttgagcca gcgtcccgtg atttcttccg
    15361 agcggcctgc gagcgagaaa tcggctccca tcgggtacat gtcggcggtg tcgataaagt
    15421 tcacgccggc atcggccgct ttgtcgagga tggcgtgcga agtcgtctcg tctgtttgtt
    15481 tgccgaaggt tgcggttccg aggcataggc gcgagacgct gagccctgtc cgactgaatt
    15541 tcgcgaattg caagatgatt tcctagagtg cgagcaagga ggccgaattt cgcataaggc
    15601 caacattcga tttgcagcgt gggggcgttt gcaaaacccg gatcaagcct tggcgtgccg
    15661 gggctcgagg cgatgggaat cgatgtccgg ccagttctcg tcctggtcga ggaacacggc
    15721 gcaggagcgg atgcgcgaat agtcgatgtc agccgcagcg cccaggcccc agggggcctc
    15781 gccacgctgt gccgcctcgg cggccttgag cagttggcgc ctcacgtaga ggatggtctt
    15841 gtcgctctgg ctgaggtatt cgcgggtgcg gtcgacgata ggccccatgg attcctgcac
    15901 gaccacgtct tcctcgaaga aatgccggtt cgggaatccg ctgaagtggc ccgcctgcat
    15961 ggcctggcga tcctgccccc atatatcggc cggcccgctc gtcggggtga ggaaattgtc
    16021 gggatcgggg ttggcgaaac gccagaaatc ctccacttcg cccggctcga atggggcgtc
    16081 ggtgcggtag ttcacgaccc actgggtgca gtgcgtgtcg tcgatgggga cggacatgac
    16141 caggatgcgc cgcgtgggct tgcccggaaa acccggcagg aacgaaaaat aaggcgcgac
    16201 gaattcccgg atcttggcga agcgccgccc gtcgccgtag tcgcgtatcg cgccttcgcg
    16261 aaagccgtac ggccgttcga ggatctcgaa ggtcggcgag gacatcgcgg tggtcgccgc
    16321 catgttggcg agcatgcctt ccgacaaggg cgattcgctg gttggccgcg tgaccagcac
    16381 gccgctgtgc agaaaactca ggtgcgccga atcgaggacg gattcgagca attgcagcca
    16441 gttgcaccgg atgacggcgg agcgggcgtc gatctggccg ggaccgaaat ccacgaactc
    16501 gaaattgggg aagggcgtgc gcagcccttc cttgtcgatg cagacccaga cgatgccgcc
    16561 ggcctcgcgc acggcgtagt gctgcagcgg caccttctcg gcgaaggccg cgcggcgctc
    16621 gggcagctcc gtcggaacct ccacgacctt gccggacacg tcgaacttcc agccgtgata
    16681 gaggcagcgc agcccgttgc cttcattgcg ggccagcgac agcgaggcgc gccggtgggg
    16741 gcagttttcg tcgaagaatc cgacccgccc atccgtggcg cggaaggcga cgaagttttc
    16801 ccccagcagg cgcacacgca gcggcgcgcc gtcgcgctcc aggcgcgcgg agcgcgcgac
    16861 cggatgccag tagcgccgca gtgcccggcc catgggcgtc gagggcccga ccctgcacag
    16921 tttttcgttg ttttcccgat tcagcattgc gaggtccccg ttcgacctga aggtacgtgg
    16981 ggagtgtctc aaatggcgga tcgatgaaca aagtatttaa cgttataagg tcaatcgcgc
    17041 cgggcctttt aaaaaaggcc tgccgtcaat tctcggcggt gatgccggcc tgcttgatca
    17101 gcgcgttcca cttgctgtat tgcgactgga tgaactggcc gaattccgcg ggtgtgccgg
    17161 gcttggtgat gacgcccaag tcgcccagca ccttctgtac gtcggggctg cgcagcgcgg
    17221 tgttgatttc gctattgagc ttgtcgacga tctcgggcgg cgtgcccttg ggggccatga
    17281 agccccacca taccgtggcc tcgaagtcgg ggtagccggc gtcggcggcc gagggcacgt
    17341 cgggcagcag cggcaggcgg gtattgctgc ccaccaccag ggggcgcagc ttgttcccgc
    17401 ggatttgcgg catggcctgc ggcgggtcca gcagcatgaa ggacacgcgg ccggcggtca
    17461 ggtcgatgat ggccggcgcg ccgcccttgt agggcacgtg cagcatactg accttggcca
    17521 ccgacttcag catttccgag cccaggtggg ccgaactgcc gttgcccgcc gaagcgaagg
    17581 cgatcttcga ggggtcggcc ttggcggcgt cgatgatgtc cttgagcgac ttggccggcg
    17641 aatcctgcgg caccaccatg atgagcggcg acgagcccag gtaggccacc ggcgtcagcg
    17701 tcttgatctc cgagaaggag tcgttcggat acagcgacgg gttggtcgcc agcccattgg
    17761 cgccgaacag cagggtgtag ccgtcgggcg cggcatgggc ggcgtagacg tcgccgattc
    17821 ggccgttggc gccgccgcgg ttctcgacga tcacggactg gttcatctgc tggccgatgt
    17881 gctggcccac caggcggccc agcatgtcga ccgcgcctcc gggaggaaag ggcaccacca
    17941 ggcgtatggg cttgctgggg taggacgact gcgcctgcgc ggccgggccc agggacaacg
    18001 cgcccagcgc cgtggcggtg gccagcagcc gcgatgacat gcggaaagaa agcaacttca
    18061 tttttcgatc tcctctctga aacttatttg ttcatgcggg ccagcgcagc gcctgagcgg
    18121 ccgccttgcc catcacccat tccagcgact cggcgtccag gaagtccagc tccttgagga
    18181 aatgatcgac gcattcccca tagctgcacg ggatccgcgt gtagtccgag ccccacatcg
    18241 tgcgccgcgg gccgaagctt tcgtagatgc gccggaggta gggcttgagg ttggcgaagg
    18301 gatagggttg ggtgctgtag cacggcacgg ccgaggcctt gacgtgcacg ttgggaaagc
    18361 gcgccagcgc cagcagatgg tccaggtcgg cgaagcatgc gtcgtcgcgc aggtcgctgc
    18421 ggcgcgccat gtggtccagc acgatggtca ggccggggtg gcgttcggcg atggcggaga
    18481 tcttgtcgac gtggccgggc acgaacagca tcagcgggat gcccaggcgt tcgcagtcgg
    18541 accaatacca gtccagcgcg ccgggctcca gccagccggc ccattgcggc ttgtggaagg
    18601 tcatgcggat gccgagcatg ccgggctgcg ccagccagcg cgccagccgt tcgcgcgcgt
    18661 cgccggtgtc ggggttgaag cggcccatga tggccaggcg gtcgggatgg cgcgcggccg
    18721 attccagcgc ggtgagattg ctgtcgccca ccggcgaggg cggcacgatg atggcgcgct
    18781 gcacgccggc ggcgtccatg gtggccagga attcctcgtc ctgcagcggt tcttcccgg
//
LOCUS       DASWPO010000093        78424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2341058, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000093 DASWPO010000000
VERSION     DASWPO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 78424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78424
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341058"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VGC69_05450"
     CDS             complement(<1..285)
                     /locus_tag="VGC69_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0294764.1"
                     /translation="MTASPPRPRSAGPVLALLVNAFVWGVSWWPFRHLQAMGLHPLWA
                     TAQIYVLSALAIIAWRPGSLRQLLRHRALWLIFLASGATNATFNWAVSIGD"
     gene            complement(287..673)
                     /locus_tag="VGC69_05455"
                     /pseudo
     CDS             complement(287..673)
                     /locus_tag="VGC69_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
     gene            677..892
                     /locus_tag="VGC69_05460"
     CDS             677..892
                     /locus_tag="VGC69_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294765.1"
                     /translation="MLGHASPAAQLAWHVYDDAYHDFDWPGLRTHEAPAFRTRAGVVP
                     IEGMNPAAREDALHRVPEYLMPYLLEP"
     gene            complement(931..1728)
                     /locus_tag="VGC69_05465"
     CDS             complement(931..1728)
                     /locus_tag="VGC69_05465"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY0294766.1"
                     /translation="MAHTLNAERGLDGTAAGRLAALDAHAVREARLDLAASLRMAARL
                     GMGEGICNHFSAMLPGSDEFFLVNAYGLAFDEITASNLLVCDFHGNVAQGQGRPEATA
                     FYIHARVHKQHPRARAVFHTHMPNATALCLVEGDPLLWASQTALKFWGRVAVDDDYNG
                     LALDESEGDRIAAAIGEADIVFMKNHGVMVLGKSIALAWDDLYFLERAAEVQRLAATT
                     GRRLKPVPPAIAAKTAAQTLAEDPASARAHLDSVRRRLMKHDPEFSL"
     gene            1970..3277
                     /locus_tag="VGC69_05470"
     CDS             1970..3277
                     /locus_tag="VGC69_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294767.1"
                     /translation="MSNTALDSSPAATGRQRSLTVVRDCVAATLGAFFEWFDLLVYAS
                     FALPIAKVFYHEGDADKAFLLSTLTFASAYLARPLGAIFLGIYSDKQGRRAALMLSAV
                     LMLIGTAMIALLPGYDTLGSFAPVGLLFARVLQGIAAGGEFGSVNAYLTERFPKLRAL
                     MSSLQFSMTGLALLVASLAAYAINHYLSPEQVMRWGWRLPFLFGCLIAPVAFYMRSHI
                     SETEAFLAETAAQRAALGSSLGRHWKAVILGALVVAGGNAASYLNIFMPNFAIKNFGL
                     SAEVAFTAGALSAVVCIVFPLVAGRVADRAGFSRTMTIAMIVGLIGLGPVYYNLMQHP
                     GLPALALFQCFMSLVFYSFYYSPVGALLADLFPTQCRTMGVSVAYVISQSLFGGLTPL
                     VVGMLVVGTGSNFAAAGYLLAIGVVAIVALRVSRLLPAYADSP"
     gene            3440..5035
                     /locus_tag="VGC69_05475"
     CDS             3440..5035
                     /locus_tag="VGC69_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0294768.1"
                     /translation="MSVQIRTRIGLTLGLLSCIMLALGVFALYATFRANSIIQEVHNS
                     DMAGAADIALIQLGVSRQRLALDRAVLVAGTPEATERVATLANRQKQMDDAWQHFQSL
                     PHDAEAARLAGVFGTAATNLRNAVQALASAVRANDQAHLIDLMRDLTQRYETLSSSAD
                     ALNKYQNDQATSRYEGAQAAHAIFRIVAISAVVAALLLTLWSYIALRRAIVRPISAVL
                     AQLRHIASSDLSHRVPAHADDELGRVLDGLNDMQARLSATISQVRASGESINAATREI
                     AAGNMDLSSRTEEQAASLQQTASSMNGLTGIVRQNADSADQASTLAASALSTAGQGNQ
                     VMVRMTDTMGTIGQSSREIGEIVTLIETISFQTNILALNAAVEAARAGEQGKGFAVVA
                     GEVRALAQRSATAAKEIKALIGTSTERVRQGMALVGEASGTMQEIIASVQRVADLMGE
                     IAAASAEQRRGIEQVALAVTQMDEMTQSNAALVEQSAAAAQSLDEQAHHLSQAIAAFK
                     TSEHAVPAAGRQSAALLEMAPSA"
     gene            5173..7530
                     /locus_tag="VGC69_05480"
     CDS             5173..7530
                     /locus_tag="VGC69_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0294769.1"
                     /translation="MKRHIKKTAQAMLLSVAAVASTQAWAAADTQTTVSGTVTDSNGK
                     PVAHADIKLTSSNGAEAGQATTDAAGRFAVEHVTPGTYAVAVSASGFAPTMSMAQPSG
                     DHPATVALILEKNDALDVQVNAKRLDQARNGLLPETGSSISRITQEDIQAMPQGESTP
                     LNQVLLQAPGVVNDSYGQLHVRGDHADLQYRINGILIPEPISGFGQSLDTRIIDQVNL
                     LTGALPAEYGYRTAGIVDIRTKSGDLGNGGSIDVYGGSHQTLQTGANVYGSEGAFSYY
                     FGGSVGENNLGIEAPTSNSNPLHDHTRQGSGFGYMSYMIDPLTRVSLMFGTASNQFQL
                     PNTPGLAPNFTLAGTPTFNSANLNETQSELNNFAVASLQGSNGGALDYQVAFFTRYTR
                     TQFNPDPLGDLIFNGVASSDFHSDTANGVQADTTWRLNDRHTIRTGVFLQQERASFND
                     DVSVFPVDTDGNQTSSVPFNIADSSSKTGYLYSAYAQDEWQINDRLTLNYGLRYDGMN
                     QYVQASQLSPRIGMSFKLTPTTTLHAGYARYFTPPSFELVPNSTINNFSGTTNQSQVT
                     QNDPVQPERDDYFDIGVSQRLGSDVTVGVDTYYKKAHNLLDLGQFGAALIYAPFNYQY
                     GRVYGAEFTANYKHDGTSAYMNLAFSKAQGMNVDSAQFNFDPDELAYISNHWVHLDHD
                     QLITASFGGSYAYRNTTFTMDATVGSGLRDGFANSGKLPLYAQINVGAIEHMNLPTIG
                     KFDARLVLSNVFGRTYELRDGTGIGVQAPQYGPGRAVYVGMTKYF"
     gene            7556..8179
                     /locus_tag="VGC69_05485"
     CDS             7556..8179
                     /locus_tag="VGC69_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0294770.1"
                     /translation="MQAWLGIIEAIRLGGWIVYPLTLLAIVALIIMMDRAYVLWRFAT
                     VPHAGANAAADIRGVLAALPAQHAARRLLAPLVADTTRPVWWIEEKARAVALEVQRDM
                     SRGLWVLETIVTAAPLLGLLGTIVGMMHAFQLIGNQGLVNPTGVTGGVAQALVATAIG
                     LVIALVALFAFNYFSRRIDRLVEELEVFANAWLSDVRLQREAAERAA"
     gene            8176..8577
                     /locus_tag="VGC69_05490"
     CDS             8176..8577
                     /locus_tag="VGC69_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0294771.1"
                     /translation="MKLRRSRAAKRGRIEIIPMIDVMFFLLATFILASLSMQRLDALK
                     LDLPQGQAQSIAEQQPLTLSVTRDGQLAINRQPVRLGDVAATVRRLVQPDGNIVVAAD
                     TAAANGIVVQALLQARQGGAEHFMIAVERGQ"
     gene            9017..9367
                     /locus_tag="VGC69_05495"
     CDS             9017..9367
                     /locus_tag="VGC69_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0294772.1"
                     /translation="MPASPAQAAAPSSPSTHEAPAADPGASGTGPARAILQPLPALPD
                     DLRQEAFQAVARVRLSIHPDGSVSVELLDPTRNPRLNQILLNTLAKWRFFPAMKDGHA
                     IESSQEIRVHFNVN"
     gene            complement(9391..10293)
                     /locus_tag="VGC69_05500"
     CDS             complement(9391..10293)
                     /locus_tag="VGC69_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294773.1"
                     /translation="MLNRLEILRIFSAAAGSPTFREAATRLGVSPQVVTRAIRELEAA
                     LGETLFHRSTRRVQITAFGQTFAAKVQSTLISVDELFDSSISTSDEVSGTVRITAPAG
                     FGRRYVLPILSALMIRHPGLRLDVRLSDTPSAVVDEQIDIGVRAGAIEDNRFVARVIG
                     PLPMWVVGSPELIARVGEPRNLQDLQSTPTTTLIDRATGRPWPWIFRGEAKFQPRNPV
                     FVTDDPETEIEVARAGVGFSQCAEYLARPYVEAGRLARILPRFEPAPWKLHVYRPQRG
                     PVPKRIRIVFDELAAQLAHATSRD"
     gene            10462..11238
                     /locus_tag="VGC69_05505"
     CDS             10462..11238
                     /locus_tag="VGC69_05505"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0294774.1"
                     /translation="MNASTTQRQLEGKVALVTGASSGIGRASALELARRGAKVVVAAR
                     RKDELDRVVRQIVDADGTAAAIAVDVAKEEDIKKLVGFTVATYGRLDVALNNAGTEGV
                     FSPLLEQDAARYDMVFEPNVRGVFNSMKYEAEFMLKQGSGSIINTASMGGLIGFENAS
                     VYIASKHAVIGMTKTASIEWFRRGVRVNALCPGLIETPFHHRGIWPSVQAQQQFAAGT
                     PAGRWGTAEEMATIVAFLASDDSSYVSGHSLVADGGYSIA"
     gene            11403..12218
                     /locus_tag="VGC69_05510"
     CDS             11403..12218
                     /locus_tag="VGC69_05510"
                     /inference="COORDINATES: protein motif:HMM:NF015159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0294775.1"
                     /translation="MSINHDRRKLLTLAGGLAVSGALPSIAQAAPAAARVVLGVTPLP
                     PYYDIIELIQPKAAELGLDLVVKKFEDTRQLNTLLANGDLDANFFQHVPYLEQYNKDN
                     NLDLVVAAGVFIGLFGAYSRRFKKVEDLPEGALLVYPNDPSNSGRALDMFAKHRLLRL
                     KDGAGIKATQADIIDNPKKFRFRGVEQPMLVRTLDDADLVAINAPFVVAIGLNPLVDA
                     LISEDKSVFVSSLVSRRDNVKSDGIVRLAQAVTSPEVKRLLEDKFQRSVRPVF"
     gene            12260..13033
                     /locus_tag="VGC69_05515"
     CDS             12260..13033
                     /locus_tag="VGC69_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294776.1"
                     /translation="MIELSHVSKRFQTADGDVDAVSDVSLRIGQGEIFGIVGFSGAGK
                     STLLRMINLLERPTSGRVVVGGADLLALPPDGLRQARQSIGMIFQHFNLVANRTVEDN
                     VSIALEIARVPQDRRGDIVHESLRTVGLADKARHYPGQLSGGQKQRVAIARAIANRPK
                     VLLCDEPTSALDPQTTGEILKYLKAVNDGAGVTIVIVTHELGVVNRLCHRVAVMEKGE
                     VRDEIDFQDSDRTLSSVLGLAILEERRRDKDYRDVYAFV"
     gene            13047..13676
                     /locus_tag="VGC69_05520"
     CDS             13047..13676
                     /locus_tag="VGC69_05520"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEY0294777.1"
                     /translation="MLQSLWETATMVGLAVGAAIVLGLPLGTLLYLTRRGGLLEHATL
                     YTTINSLVNLIRSFPFLILLVALIPITRFVLGTSLGTVAASVPLGVAAIAYYSRLSEQ
                     SLLDVDAGVIEAAQAMGATVWELLFKFLFVEALPGLVLGLTISIISFISYSTLAGAVG
                     GGGIGDFAIQYGYYRFETDVMVATMIIIAVIVQAVQFGGSRLSRHFDKR"
     gene            13696..15066
                     /locus_tag="VGC69_05525"
     CDS             13696..15066
                     /locus_tag="VGC69_05525"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108667.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HEY0294778.1"
                     /translation="MATETRALHFNLNFINSGTHSSAWRWPGNDPRAFIDPDFYVAVA
                     KTAERGFFDAIFFPDNPALTQEPRFRPFNALEPTLLLATIAARTGHIGLIATMSSTLN
                     EPYNVARRFASLDILSNGRAGWNVVTTADRGASANFGLQDTLAHARRYARAAEFTHVV
                     KSLWDSWEDDALVADPDSGAFIDTRKIYPLNHHGEFFDVAGALNLPRPPQGRPIVVQA
                     GGSNDGRDLAAAHADAVFTLAYTLEEAVAYTQDIDARARRFGRTRDDIRIFPGLVTVI
                     GGTEAEVRQRLEVHKELSSFEYGLARLAGSLRIDPKSLALDEPLPKDLALHPDHNHTF
                     FKGIVELAAAKGGLTVRELVYHQLGGTSHRIIAGTPEQVADDIETWLRSGAIDGFNVM
                     PDVLPSGLDAFVNHVIPELRRRGLYRDGYPSHTLRGNLGLDRPANPRAGQPSLAEQEH
                     VAGQFF"
     gene            complement(15776..16690)
                     /locus_tag="VGC69_05530"
     CDS             complement(15776..16690)
                     /locus_tag="VGC69_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294779.1"
                     /translation="MITFKQLEAIYWVTRAGGFAQAAQELHTTQSAISKRIQELERRF
                     DTELFDRTSRNARLTPRGEQMFRLAQQLLLQRDEAVERFAQASVLETEIRIGVTELTA
                     MTWLPRLAMAFQAQYPRVAIEPHIDISVNLREKLLADEVDLMIVPDVFRDHEDAQLNF
                     EPIGKVELAWMCKPGMVARGTPIKLNELADHKLIIQGLTAGTGLVYKRWLRTMGVRPA
                     RLIVCNSLVGRIALTVSGLGLSYLPLKSMEPLLRAGTLDVVRATQPLPPSVYVAAYKR
                     DRRSALVTSVTQLAIENCDFSSMVQMAY"
     gene            16858..17826
                     /locus_tag="VGC69_05535"
     CDS             16858..17826
                     /locus_tag="VGC69_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294780.1"
                     /translation="MKSISRSLALVLFAVVALNARAAGYPDQPLRLIAPYAPGGNIDS
                     TARLLAQGLSQRLGQPVIVENRPGAAGVVGSEVVAKSKPDGYTMVLNSTGALASAKVL
                     NPGLKLDPLKDFAAAATVSQAPMALIVNPALPVRSVAELIDYARKRPGKLTMASSGIG
                     TSAHLAGEMFQQMTGVKFLHVPYAGSSQAVSDLVAQRVDLTFDQLTSTLPLIKAGKLR
                     ALAVTTSTRSIFMPELPTLEESGVHGFEMSTTTGLLLAKDTPPAILDRLNRDSAAILN
                     DPAVKDKMLLLGAAPLPGSGNDFDRMLRGEIEKWTRVADTADIKLP"
     gene            17848..19059
                     /locus_tag="VGC69_05540"
     CDS             17848..19059
                     /locus_tag="VGC69_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0294781.1"
                     /translation="MQQDTNTQGPLAGIRVLELGTMIAGPFSATLLADFGAEVIKVEQ
                     PGVGDPMRNMSPFVKDESLWWNVEGRGKRSITVDLHVEDGQELVRDLAVHCDLLVENF
                     KPGTMARWGLGFERLHDLNRRLVMMSVSGYGQTGPYAGKRSYDRIAQAFAGMLNATGF
                     PDRPPVRPGFSIADFGTAVFGAFAAMMALYHRDARGGQGQHIDVAMYEAMFRLTESMA
                     ISYDQLGAVRQRRGNLNAGAAPGDHFLTADDRYLVLTISNNALFGRLCEVMGQPDLPR
                     DPRFATHAERAAYLPEINGIVGDWLRTLTAQQAGELLDAAQLPYSLIYNIADIYADPH
                     YASRGAIATVDHPRLGKLRMQGIVPRLLGTPAPALRPAPTLGQDTRDVLTSLLGMTQA
                     QIEQLYAQKII"
     gene            19073..20230
                     /locus_tag="VGC69_05545"
     CDS             19073..20230
                     /locus_tag="VGC69_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0294782.1"
                     /translation="MGPLAGVKVLEMEALGPVPWAAMMLSDMGADVLRIDRPDGGRPD
                     PLGPRSGQVMLRGRRSLVLDLKQPGGGEAVLELAARADILLEGLRPGVMERLGIGPEI
                     CLARNPALVYGRMTGWGQHGPLAQQAGHDINYIAAAGVLHTIGPHDGPPAVPLNLIGD
                     LGGGGMLLALGVVSALLHARACGEGQVVDAAMIDGCNALMASVWGRFAAGAWCDERER
                     NVLDGGAPFYSVYEAADGKWLAFGAIEPHFYQALLDGLGLSGETLPAQHDRTAWPDMR
                     RRIAGIVRQRSRDDWCAIFAGSDACVTPVLSLAEARESAHQRARDAFVAIEGVMHPAP
                     APRFSRSAGAIVRPPCARGAGGAEALRDWGLDPSHPALRQVWNQPPSGRES"
     gene            complement(20352..21254)
                     /locus_tag="VGC69_05550"
     CDS             complement(20352..21254)
                     /locus_tag="VGC69_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294783.1"
                     /translation="MNPRQLVPSMSQLLAFEAAARHESHSRAADELFLTQGAVSRQIQ
                     ALERLLGVELFRRDGRNAALTDAGRLYLQELSPALNQIRGATLQAIALRSGRGTLRLA
                     TLPTFGSKWLLPRLHHFYSTHPGMLIHLHSRIRAVDFNTSGIDAAIGVGSDDWPGLTA
                     HRLHTEQQVVIASPSAIDAGLRLTQESLGSHLLLSVATHPQGWNEWLGHHGLTPRGMR
                     IGPSFELTSHLIQAVMADIGIGLVPRVLVEDELQQGQLVMVGNAVTSRRSYYLVYPPR
                     NTSLPSLVAFRAWLLNDSDIPAEG"
     gene            21453..24482
                     /locus_tag="VGC69_05555"
     CDS             21453..24482
                     /locus_tag="VGC69_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEY0294784.1"
                     /translation="MISRLAPAVSPSQPYRVFLDALAASGFRGEIDAGAAMRTVLATD
                     NSIYQRLPQAAVFPRDAEDVQSIARLLALPAHRRVAVAPRGGGTGTNGQSLTDGIVVD
                     LSRHMNRILEIDPAARRVRVQAGVVKDQLNAALRPHGLFFAPELSTSNRATIGGMVNT
                     DASGQGSCTYGKTRHHVLALDCVLLGGERLASRPVAEGELAALCAGAGRAGQVYRTAQ
                     RIHAERADEIAAQFPRLNRSLTGYDLAHLREPDGRFNLNSVLCGSEGSLAMVVEATLN
                     VLPIPRHTVLVNVRYGSFMDALRDAQALMAHRPLSIETVDSKVLMLAMQDIVWQGVAQ
                     YFPEDGAQPVRGINLVEFCGDDPAELRWRTEDFLAHLRADHSVLRLGHTLAEGHAAVE
                     RVYGMRKRAVGLLGNTPGEQRPQPFVEDTVVPPGHLADYIAEFRALLDGHGLEYGMFG
                     HVDAGVLHVRPALDMKDPRQAALVRPISDAVAGLTRKYGGLLWGEHGKGVRSEYVPDF
                     FGPLYPALQALKAAFDPANQLNPGKIATPADGGAALLRIDGVPLRGEADRQITPGVWL
                     SYGSAMHCNGNGACYNFDPDDAMCPSWKATRDRVHSPKGRASLMREWLRQQQAAGADV
                     LAPRRGWLAGLPARWRNDWARRRGEADFSHEVYDAMAGCLACKSCAGQCPVKVNVPAL
                     RARFLALYHTRYPRPLKDYVIGSMEYTVAGLSRLPRLYNAIMGAAPVRAALARRVGLV
                     DSPLFSDLDWRGHLRRLGVRPATVRRLAALTEAQRARSVVLVQDAFTRYFETPVAADF
                     VELAVRAGYDVYVAPFLPNGKPLQVHGFLDGFERAARRNAVRLRRLADCGVALVGLDP
                     AMTLTYRQEYPQVLGEESCPRVLLPQEWLAQALPARGGRPAAALSYRLLAHCTERTTA
                     PASAAQWRAVFERAGLSLALQASGCCGMSGTYGHEVRHLETSQAIFAQSWSAFVDAAS
                     EDGEDELLATGYSCRSQVRRLRGRALRHPVQVLLDYY"
     gene            complement(24544..25695)
                     /locus_tag="VGC69_05560"
     CDS             complement(24544..25695)
                     /locus_tag="VGC69_05560"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0294785.1"
                     /translation="MMTPTPHAGPSAWTAEQARQDDSWIQRLTPQEAEGFDLALRHAK
                     AAAKPLLSMEQADYPLPEASQAALRRAIDGTQGRWGMRLVKGLPTDAWNEEETRIAYW
                     GMSLYMGVGRTQNRASQVINDVRNEGLDYKVKGGRGYNTNAGLDFHQDSCDVVALLCR
                     RTAKAGGTSKVISSMALYNEVQRRRPDLIPVLEQPWFHSYQSTQDPSQPPYYRCPIFG
                     SDPEYFCARTNRKNTDAVQRDYPEVPRLTPQQIQALDLMDELMPSPLLCHTMELERGD
                     LQLLNNYVTLHSRTAFEDYDEPDLKRHLLRLWLAVPSAQRLPADWEEYYGDVRAGAVR
                     GGVRGIAITQEFLAYEERQARTLRMPWRPWQPKVRKEDMEALLGTLATA"
     gene            25788..26681
                     /locus_tag="VGC69_05565"
     CDS             25788..26681
                     /locus_tag="VGC69_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294786.1"
                     /translation="MKIEALHTLEAILHTGTFAQAALERNVTRSAVSMQVKQLELYVG
                     QPLFDRSGLYARPTSLARELVYTMHDALARLETLRKRPSVAVEGIVRLGVIESMQPIV
                     LPGTLKWLAERHPRLEIRPARGRSSTLMTAVRSGELDAALVAQPPEGASARLRWQPML
                     SRELMLLAPPEAGEASVQALFRLYPWIRYDRKTVSGSVASHYVAQQIGDVRGALEFDS
                     ATAILAMVSAGLGVAVIQLSEPALLQSYPVRIVRLGRNAPSIQYSLVSRRGDEGSRAL
                     QAMHDAMQSALVVARHPKRKG"
     gene            complement(26696..27322)
                     /gene="leuD"
                     /locus_tag="VGC69_05570"
     CDS             complement(26696..27322)
                     /gene="leuD"
                     /locus_tag="VGC69_05570"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0294787.1"
                     /translation="MEPLVRLRAVACALPQINIDTDQVLPARFMKQPRNLPEGYAPFL
                     FHDLRRLQDGAHDGQFPLNQPRNHGARILVAGRNFGSGSSREAAVYALADAGIRCVIA
                     PSHGDIFASNAVNNGLLPARVDEAAAEAMFALLAPGGVEAEVDLEACRIEVGGQGFDF
                     HVDPVWRLRLINGWDDIDTTRRYDSDVAAWLAQDALRRPWAGLPANPA"
     gene            complement(27322..28740)
                     /gene="leuC"
                     /locus_tag="VGC69_05575"
     CDS             complement(27322..28740)
                     /gene="leuC"
                     /locus_tag="VGC69_05575"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0294788.1"
                     /translation="MSTPRTPQTLFDKLWNSHVVAQREDGASLVWVDRHYVQEGSFHA
                     FNKVRARGETVARPDLTVAIADHYVPTRGRNLPIANAELTRMMDQLAANARDFGIRLY
                     DMHHPWQGIVHVVGPELGMTLPGLLVVEGDSHTSTHGALGAWAFGIGASEVAHVLMTQ
                     TLWQKRPKRLRVRVDGQLAPGLGAKDMALTIVARLGADGAVGHVIEYAGAAVRALSME
                     GRMTLCNMSIESGARCGMVAPDAVTLDYLRGRPFAPAGDAFDQAAQQWLELAGDDDAV
                     FERELVLDTSQIEPTVTWGTTPEDALPINGTVPDPDALGDPARAAHVRDAIDYMALSP
                     GMRLTDIAVDRVFIGSCTNARIEDLRAAAAVLAGRRSKVPGLVSPGSTLVQRQAEAEG
                     LDRIFLDAGLEWGGSGCSMCAAMNGDMLDAGQRCASTTNRNFKGRQGLGSRTHLMSPA
                     MVAAAAVAGRLIDMRELTQRRP"
     gene            complement(28750..29664)
                     /locus_tag="VGC69_05580"
     CDS             complement(28750..29664)
                     /locus_tag="VGC69_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0294789.1"
                     /translation="MNTDIADTSQPPICLPADHRPRPPRHALPPGATDCHCHIYEQPE
                     RYPYTPARSYTPAQATRADYLAMCAVLGIERTVQVSASVYGADNSLTLDLIAELGQHR
                     ARGVAGLPPDVDAAELDRLHRGGMRGVRVSTFVKGYGGTDAIAALAPRIRPLGWHLQV
                     HFHHADEIAALEDTLLRLPVPLVFDHLGSVRGGEGPGNAGFQAMLRILRSRDDCWAKI
                     SSWYRRSDSGGPDYADIRPLVETLVQARPDRLVFGTNWPHPALFPPDTVPNDGALLDL
                     FCDWIPDQAVRRRIFTDNPAELYGFPQG"
     gene            complement(29685..30650)
                     /locus_tag="VGC69_05585"
     CDS             complement(29685..30650)
                     /locus_tag="VGC69_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294790.1"
                     /translation="MKPFARLFACVALCAASACAQAAYPDHPIRLIVPFPPGGNIDAT
                     GRIIAKGLTEQFGQSVIVDNRPGAAGILGSEIVARSPADGYTMLLASTGALAPAKALN
                     PDLKLNVEKDFVAASPIARAPLLLVVSPSLPVHSVAEFIAYAKQQPGKLTVASSGTGT
                     AAHLTAELFQSASGTKLLHVPYKGSSEAVADLLGGHVDLTFDQLASTLPQINAGKLRA
                     LGVTTSARSSVVPQIPTLAESGLPGFESSTTTGLLLPAGTPQQIIDKINTAMQQVLNS
                     AETRRQFGDLGSDVLTGSGSDFDRIIKTETEKWTKVIQDAHITLR"
     gene            complement(30721..31587)
                     /locus_tag="VGC69_05590"
     CDS             complement(30721..31587)
                     /locus_tag="VGC69_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY0294791.1"
                     /translation="MPSDTLKARLLTAPAILAPGVYDALTALIAERAGFEALYLSGGA
                     VSYTQFGRSDVGLTTAKEAVDTLSRITDRVRTPVIVDGDTGFGNAINVQRTVRDFERA
                     GAAMIQLEDQTFPKRCGHLENKSLVSTAEMCGKLRAALDARHSPDTLILARTDAVAVE
                     GLDAAIDRAEAYLANGADALFIEAVSSAADMDRVCGIFSSRAPMLANMVEGGKTPIQS
                     VEELSGRGYRIVIFPGGTARFAARQLENYYTSLKACGTTQPMKDQMFDFNELNRLIGT
                     PELLELARSYAG"
     gene            31685..32545
                     /locus_tag="VGC69_05595"
     CDS             31685..32545
                     /locus_tag="VGC69_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294792.1"
                     /translation="MKWEAFATLDAVLCTGTLAGAAKSMNLTASAVSIQMKQLEAFIG
                     HQLFDRSGLQVKPLPLAHEVAAVMRRAKVELDGLRRPSSILLEGTVDLGIIESMQSRL
                     LPGVLQALRISHPALRVHPHRGKSAQLTNAVKAGDLDAAIVAQPESGGSSRLQWYALS
                     QYPLSLVVPPNAEAQSIPDLFKRYEWIQYDKATVAGRLAARYIHQHFKPLTSGLELDG
                     VRAVLAMVSAGLGLSVIQLSESNIAQLFPVRVVALPNPPMIQFSLVFRASDEHSRALI
                     AIRDAVVTSV"
     gene            complement(32636..33664)
                     /locus_tag="VGC69_05600"
     CDS             complement(32636..33664)
                     /locus_tag="VGC69_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0294793.1"
                     /translation="MTQTSFFNDEPTPPSSAPRARRAGGKAIPPAEHPEPLRELGAAL
                     PKDVYLGTSSWSFPGWNGLVYGAEASESLLSREGLPAYSAHPLLRSVSMDRTFYAPLT
                     QEQYARHASQVPEGFRFVIKCPNLYTDAVQRGERGVPCGPNPGFLDAAAATENFVAPC
                     VAGLGAKAGPLVFQFSPLPLDILADTAAFVEHLSAFLAALPPLPASRANEQAFYAVEF
                     RDPDVVTPRMMRMLAERGVRYCVAIHARMPSAARQMQAVAATGPGPLVARWSLLAGQR
                     YEAAKARFFPFDKIVVPDPATRRVLAQACREARAMGQPVWITANNKAEGSAPRTLELL
                     AREILALP"
     gene            33860..34192
                     /locus_tag="VGC69_05605"
     CDS             33860..34192
                     /locus_tag="VGC69_05605"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY0294794.1"
                     /translation="METLEKETVMGDRTVIGAREAMRVLPGWAAAQTRDAIEKTFVFA
                     DFNAAFGFMARAALLAERLDHHPEWSNVYNRVQVLLTTHDAGGVTRLDVRMAQFMDEA
                     AAGIHPTP"
     gene            34260..34502
                     /locus_tag="VGC69_05610"
     CDS             34260..34502
                     /locus_tag="VGC69_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HEY0294795.1"
                     /translation="MNPEDLKHLVTWTMPFGKYKGRLLADLPGHYLTWFAREGFPAGQ
                     LGRLLALMHELDHNGLKPLLDPLRGGDAVKQENRER"
     gene            complement(34509..35819)
                     /locus_tag="VGC69_05615"
     CDS             complement(34509..35819)
                     /locus_tag="VGC69_05615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0294796.1"
                     /translation="MSRKMHLGLMGGVVGSGWRLPEARIGTLDFSMVHRVVTTAERGK
                     FDLVFFADAVNSGADASPGTVMRFEPLSLISALAVTTSHIGLVATVSTTYSEPYNIAR
                     AFASIDYLSGGRVGWNVVTGSSPDAAANFGSMPHPPHAQRYEIAAEYVEVAKGLWDSW
                     EDGALVLDKKSGEFFDRTKLHELNHAGPHFSVKGPLNIARPPQGYPVMFQAGASDQGI
                     DFAARTADAIFAAQHDLDEAVAFANRLRDRVQAVGRPRDAVKVLLGVSIVMGETREAA
                     RKTIAEIAAMADMPSTIRSFADRVGYDINTLDLDGPLPDYPPSSSAMLGHAKVLRSMA
                     ERNHLTVRDLRDMLAVSSHRLLSGTAQDIADDMECWFKAGAADGFVIMLPYAPEPLDK
                     FVDEVVPILVARGLFRQDYEGRTLREHLGLARPGHPASAAQAAE"
     gene            complement(35860..36741)
                     /locus_tag="VGC69_05620"
     CDS             complement(35860..36741)
                     /locus_tag="VGC69_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294797.1"
                     /translation="MTAGKKRLGLGLMVLVMGAVSALGLAAPAGAQVVEAARAALPDG
                     IRASGVLKVATSLAWAPFAYKTEAGETAGLDIDIVNALAARLGVKPEFADLKFPTIIP
                     GVQSGRYDIGVDQFAITPDRRKAVDFVPYITGHSGLLVRQGNEGLTINDLCGKELSLT
                     QGSSQIDTATALSKKCQAEGKPEIKFTYYPDSAATYMAVANGRGDGFITGRAVGEWIS
                     RINPKMKMSMTKETLADEPYSASGIVMTKENTALRKALEAALLSMVADGTYMKLLEKY
                     GVPDSALTVDQITHSPI"
     gene            complement(36807..38123)
                     /locus_tag="VGC69_05625"
     CDS             complement(36807..38123)
                     /locus_tag="VGC69_05625"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HEY0294798.1"
                     /translation="MMAETKGKRMMRLLAYIKTGPTVAHFGGWRHPEATLNDIFEPTR
                     YEQMAILFEQAKFDGVFFADAFGIAEVFGGTFETYVKNGGQNSWLDPMTVLPLMARVT
                     TRLGLGATISTTFNNAFQVARALASLDMLSKGRAAWNVVTSSTDMEAKNAGLTEIYPH
                     DERYERADEFLEACMALWHTWEPDAFVLDKESGLFADPAKVRYADYQGKWIASRGPLP
                     TPPSPQGHPVIMQAGGSPRGRAFGARWAEMIFAPGSDKETMLAYTTDMRRRIAEEGRD
                     PDACKFLMGITPILGETYSIAQERAEFLDSLQEKESDLAYASMGAGADLGKHKTVEEV
                     VAARGNQGIYGSTDVSRIKAEKEGVTLAESYAKGQTAKAQIGTPAMVADILQDYFESG
                     ACDGFILRPMTMPTSYEQFCRMVVPELQKRGLFRTEYEAATLRGRL"
     gene            complement(38220..39107)
                     /locus_tag="VGC69_05630"
     CDS             complement(38220..39107)
                     /locus_tag="VGC69_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294799.1"
                     /translation="MNWDDLHHFAALAEAGSLSAAARLLGVEHATVARRIAALEASLG
                     VVLADRRGRRWALTAEGERIAAIARRMNHEAQAAKRSAEGARAELAGTVTISAPPALA
                     AARLAQPLVALQQRHPELAIRILGEARTASLAHSEADIALRMTRPEHGDLTLLKLGDI
                     HFRLYASPSYLAAVREADWRFVGSGGELGQSPQQQALQAYAADRPFAFHANSVELQLA
                     AARAGAGIAALPDFMAQGHPGLALIARDQPLASRDLWLVVHTDLKRAAPVRAVMEAIR
                     KAWDRPHRPSDRLDRGNSY"
     gene            39213..40181
                     /locus_tag="VGC69_05635"
     CDS             39213..40181
                     /locus_tag="VGC69_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY0294800.1"
                     /translation="MDIKIELRAQGGIDNLHVADCPPQEPGSGEVRIRQAAAGVNFID
                     VYQRQGLYSLSLPMVLGVEGAGVIEAVGAGVEGLRVGDRVAYGAMLGGYAATRLLPAW
                     RVVRLPDAVDFGIAAASFLRGMTAHMLFTHTCSVQPGMILLIHAAAGGLGATLTRWAK
                     QRGCVVIGTVSSAGKAEVARAHGADHLIVGRHADVVGEVARLTNGRGVDFAIDGLGGD
                     VLPKTFACMRSFGTVASVGQAAGPIAPIPVEALSSPRCLSLARPSIMAYCADPDRYRA
                     AAAAVVEAIGLGLMPAPGHRFALKDAARAQHELESGKTTGSIVLIP"
     gene            40328..40630
                     /locus_tag="VGC69_05640"
     CDS             40328..40630
                     /locus_tag="VGC69_05640"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0294801.1"
                     /translation="MFIAMNRFRIALGREEEFIEIWRQRDSRLDTVPGFKKFNLLRGP
                     SDDEATVFVSHSTWESGAAFEAWTKSEAFRAAHAGAGGSKGLYLGHPKFEGFEAVL"
     gene            complement(40648..41493)
                     /locus_tag="VGC69_05645"
     CDS             complement(40648..41493)
                     /locus_tag="VGC69_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0294802.1"
                     /translation="MSLKFPATLAACILALTAGCASVPRDQDASYEGALRQAVDSSQR
                     TPAFVARDAARKPYDELRFFGVRPDATVVEISPGGGYWTEILAPYLQARGTYYAASYP
                     LDAADAADAGQRRETQQFRAKLDADPARYGKVRMTPFGPGKYDIAPPGSADVVLTFRN
                     LHNWMADGYAAQALAAFYRALKPGGMLGIEEHRGRDDKPQDPKALDGYVRQDYAIHLM
                     EQAGFEFAGGSEILANPKDTKDYPAGVWTLPPTFRLGDKDRARYAAIGEADSFLLKFR
                     KPGNR"
     gene            complement(41568..43859)
                     /locus_tag="VGC69_05650"
     CDS             complement(41568..43859)
                     /locus_tag="VGC69_05650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0294803.1"
                     /translation="MKLEVAVRGLCDFTARAGDLDLRFTPAPTALEGMAGHAVIAQRR
                     GEDFEHEVALTASHAGLTVRGRADGYEPGTRRLEEVKTYRGDLDALRDNQRALHRAQA
                     RVYAHMLCASRGLGEITVAVVYFNLDTEAETAVAETLDAAALAAFFNDLCERYAEWAA
                     RERAHRQARDAALESLAFPHGKMRAGQRELAVAVYRAARDGRPVLAQAPTGVGKTLGT
                     LFPLLKACATQGLDKVYFLSAKGTGHGLALDAIARLHATREAGLPLRVLELVARDKAC
                     AHPDRACHGASCPRAHGFYDRLPAARAHALARPDATLTRESVRAAADEHGVCPYYLGQ
                     ELARWSDVVVGDYNYWFDASAMLYALTQAHQWRVAVAVDEAHNLLERARGMYSVSLSM
                     AQVRAARMAAPPQLRKPLDGLLRSWRAVQKKQATPYAAYDLVPANLCEAVRRANAALA
                     EHFASGPAAPGDPALALYFTLLQFDRLAASFGNHALFDLAVRDAAGTSAKPESEICIR
                     NVVPAPYLVERHAAARCTVLFSATLSPRDFHLDTLGLPANTDWVDVDTPFSAHQLSVN
                     VVTTVSTRWHDRARSLAPIADLVAAQYADQPGNYLVFFSSFAYAEQVAECMARAHPDV
                     PLWTQAPRMNDHERASFLARFTEDGRGVGFAVLGGAFAEGVDLPGRRLIGAFIATLGL
                     PQVNPVNQQILASMGARFGQQFAYDYTYLYPGLQKVVQAAGRVIRTATDTGTVHLIDD
                     RFRQRKVRALLPSWWHLSADPPP"
     gene            complement(43856..45529)
                     /locus_tag="VGC69_05655"
     CDS             complement(43856..45529)
                     /locus_tag="VGC69_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEY0294804.1"
                     /translation="MFPAYRYYYLHNFQHALDWLAARYADVLAPAELDFLRAFPGLPM
                     ASRALLVRLLMRRCAVYRAERLQYEEIGPVAPAAEPLLALGWLRADPTLAWSDWAALH
                     TKEELTRRYPQAGLRPALRKAELLPRLAAFLGEPHARPCSAWGAAPGEAIWSVDVAPL
                     AQRLRLMFFGNLHQTWSEFVLADLGIFQYEKVEIDAANRAFQQAADVDVYLALQAVRD
                     SLETARPGEAPALRAQLAACETAVPWLARRQAKLWFRLGQWCERAGDWTEAEQAYRAT
                     AYSGARQRLIRVLERQQRFAEAHTLARQTLASAASEAERQQVSRMLPRLARQAGQPAP
                     AQRARRAADATTLTLACPDGASRVEIVARDHFAEDGSQVHWVENGLVNSLFGLLCWDA
                     IFQPVPGAFFHPFQQGPADLHEPDFRARRAQAFDACLATLRDDGDGGHRARILARFEA
                     KAGIQSPFVFWGMLTRELLGTALDCIPAAHLSLWFARLLDDLKTNRSGLPDLVRFWPA
                     QRRYEFIEVKGPGDRLQDNQARWLEYAAAQGLPLRVCHVRWATGEEQAA"
     gene            complement(45695..48838)
                     /locus_tag="VGC69_05660"
     CDS             complement(45695..48838)
                     /locus_tag="VGC69_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY0294805.1"
                     /translation="MAATLPAYAELQCTTNYTFLRGASHPEELVARAIELGYAALAIT
                     DECSVAGVVRAHVQAKKHKFPLLVGSSFVLRNADGKPAVQLTALAQTRRGYGNLCELI
                     TLARGRASKGEYRLMPSDLDQPTEPSYAHLRNLPECVVLLTPEYGAPLERLLAQAEWA
                     AATFPGRCWMALTLLHRAQDDRHRAHVVEAARRCHLPLVATGQVEMHGRSRKPLHDTL
                     TATRLRTPIAECGFERASNAEQHLRSRLRLANLYPRELLEQTLEVATQCPFNLDELRY
                     EYPDEVCPPGKTPAEHLRQETYLGARRRFRQGMPPQVQAQIEHELALIADLKYEAYFL
                     TVYDLVSFARRRGILCQGRGSAANSAVCYCLGITEVDPARGTSLFERFISRERNEPPD
                     IDVDFEHQRREEIIQYIYEKYGKDRAALTAVVISYRTKSAIRDAGRALGVDPMLVDAV
                     AKSHHYWDGRANLAARMAESGLDPESPVAQQWIVLAERLMGFPRHLSQHPGGFVISRG
                     PLCRLVPIEPAAMAGRSVVQWDKDDLDAVGLLKVDVLALGMLSVLRRALDLVSAQRGD
                     VFELQDIPPEDEKTYDMICAADTIGTFQIESRAQMSMLPRLRPRKYYDLVVQVAIVRP
                     GPIQGGMVRPYLRRRQKLDPVTYPSDDMRQALERTLGVPIFQEQVMQVAMLAAGFTAG
                     EADELRRSMAAWKRKGGLGKFYDKIIVGMTERGYDRAFAEQIYQQIQGFGEYGFPESH
                     SASFALLAYASAWLKCHEPEAFLAALLNSQPMGFYSPSILVQDARRHGVMVLPVDATR
                     SNWDAQLEACSAARPAVRLGINQVKGMRQEAAWRIEVARAVHPFASVADLARRADLSR
                     HDLQALAAADALRPLAGRRREALWDAIVSAPERGLLREAEIRETARPQLPGMREGQAL
                     VADYQHLGLTLGRHPIALLREQLTALRFAPSSQLRDMPNRRLVRAAGIVTVRQRPGTS
                     RGIVFVTIEDEDGQVNLIVRPELVDRQRRELLGARLLGAYGSWQSKSGVQHLVVGRLV
                     DLTPLLGELAISSRDFH"
     gene            complement(48845..50293)
                     /locus_tag="VGC69_05665"
     CDS             complement(48845..50293)
                     /locus_tag="VGC69_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY0294806.1"
                     /translation="MMPVWIAVHLPHASLDSQCPRWRTEAAVVLEAGQVRACSPLAAQ
                     AGVRPGLRASGVNALCPSATLRAYDAQAHARLVEDAALALLQYTPEVALTEADTILLD
                     VTASLNLFRGIRALCHRIAATAQGLGVAARLGVAPTASGAWLLAASARQRRRVLKLTT
                     LARRLDALPAACLPAAARHLDWLAGIGCDTLAALRRLPRGALQRRTHAALLQALDQAY
                     GEAPELHAWMAAPAVFAGRIELEERVEHVAGVLCVASRLVEQLCGWLVAQHRAATGIA
                     LVIEHERGRHACAPTTLDVRLAGPSWSPARMMPLLTERLNRLALAAPALAVTLQAVDL
                     ETLAPVPMDLFPEQGGNTADHAHVMALLVARLGRENVLHPSPVADHRPETANRWVPNN
                     APAGPARYAPGVTRPCWLLESPIALSTRGHRPYYGAPLRIVQGPERIEDGWWSGLIVR
                     DYFVAEAPDGARYWLFRARGEASGWFLHGLFG"
     gene            complement(50220..50930)
                     /gene="imuA"
                     /locus_tag="VGC69_05670"
     CDS             complement(50220..50930)
                     /gene="imuA"
                     /locus_tag="VGC69_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEY0294807.1"
                     /translation="MTSASLAPEHIHPALWRASQLSRAPGRYAPTGIAALSAQLPGGG
                     WPRGNLTELLVQQAGVGELHLLRSALLGPDARPIVLVRPPHAPQAAAWAGWGADPARL
                     VWLRPERDVDALWAAELAVRSSAFAAVLLWQNAMRAAAVRRLQLAAQSGDALFVVVRP
                     LAAAAQASAAPLRLALAPVPGGVSVRILKRRGSVCDAPILVPLYPARRIAEVDDASLD
                     SRAPAAREPGQSVPTLAN"
     gene            complement(51123..51605)
                     /locus_tag="VGC69_05675"
     CDS             complement(51123..51605)
                     /locus_tag="VGC69_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEY0294808.1"
                     /translation="MSMIEFLTARTFKTGHISSLRLFRISIPISHCKGYTQMPLVELV
                     ERQWQDYARTHRLRSTLLVHLAAVPAFIAGNVAMVAALLLGMWIAAMLCLAAMAASLA
                     WQGRCHRGEPGQPERFSGPAEALVRILAEQWLTFPRFVLSGGWTRALREASRQPLPHG
                     "
     gene            51598..52362
                     /locus_tag="VGC69_05680"
     CDS             51598..52362
                     /locus_tag="VGC69_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0294809.1"
                     /translation="MLITPSSGTFPRPSAMVVWGAGDASSAHRHHSVQLILALAGNLR
                     IRGGEGEPWHCCDGALVRADALHEVDARGATVLIGFIEPESRLGAALAERIAADITHL
                     PADEAARWRSTIFAAGSPPGDEKIKRWLEADLLDGRPQPTLHPGVSRVLRHVRHHLQE
                     PDALSLSALAVVAGLSPSRLMHAFTASMGIALRPYLLWSRLQLACGELMRGASTTEAA
                     LQAGFADAAHLSRTCRRMLGTTPTAIAASKARSNID"
     gene            52549..52959
                     /locus_tag="VGC69_05685"
     CDS             52549..52959
                     /locus_tag="VGC69_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294810.1"
                     /translation="MNKGFAGISRVGTLTQGANQNFKPLASGLELNGVRAVLAMVSAG
                     LGLSVIQLSEPNISQLFPVGVVALPDPPMIQFSLLFRASDEHSRALIAIRVAGRGWEE
                     NYAALRLSSATASCFPRTKTIQAFDALNITLYQN"
     gene            53111..54640
                     /locus_tag="VGC69_05690"
     CDS             53111..54640
                     /locus_tag="VGC69_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294811.1"
                     /translation="MKSSRRDFLTTLAATATVGTMLPGYSLAAETPKRGGHVRMAIQN
                     GSVADTLDPAKGIHSGDFTKQFCLFNALTEFNNSLTAELALAEHLESTDGSNWHILLK
                     QGVHFHNGDELTAEDVVFSLARHKDPATASKVFSIASNFKEIKAVGKYEVMLALEQPN
                     FDLPLILGSSFFLIIKNGTKDFTKPVGTGPYLVDTFVPGGGFSAKRNPNYWKTGLPYL
                     DSVEIIGVPDNAARVNAVLAGDVDICSLVDHRYADQVKGNNNVQLIVNKLGIYTDLIL
                     RQDHPITGNPDFVSAVRYLQDRNRLVNTAMRTYGLVGNDHPVAPWDPYFLEGLPQRSF
                     DLDKAKYFIKKSGLAGQSMEVICQPGLSPSVEGALFLQSLGEQVGFNFIVKQVPVEGY
                     WANYWTKYPITYGSVTNRPTVGMIFELYYGSKSPLNEAKWRDPKLDQLLTSAYAEGDF
                     KKRKAIYGDLQTLVHESAGTLVPVFDVILDGVSTTVRGYYTRPSGMNMGFRFAEVVWR
                     V"
     gene            54686..56734
                     /locus_tag="VGC69_05695"
     CDS             54686..56734
                     /locus_tag="VGC69_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0294812.1"
                     /translation="MISISADVGGTFTDLVLVDTESNLRLVEKVPSQKGTAAAVLEGI
                     GLLLARSGKKASDITRFIHGSTIATNAWLTRNGTPSALLVSRGFRDTLEIKDQRRKHL
                     YKLDDRRASPLIPRSRVIEVAERIDALGHVVLELTQAEVERIGKIVAALGVESVAISL
                     LFSHLNPRHERLLAKSLVDRCQGLLVYCSSEINPQLGEYPRANTTAAASYVGPELQRY
                     LLSLEQGLQEMGFSFPLLLMRSDGGVATLRAVLANPATMLLSGPAGGVVASLDLARRA
                     GVRNVITFDMGGTSADFSLIYQQSIGTSSERVVDDQILRVPMLDIETISAGGGSIASV
                     DHAGALHVGPQSAGSYPGPACYGRGGKLPALTDALAALGILQPADFEHAQTTLSANAA
                     REAIEKEVAIPLGLSADESAYGMIAVACAHMRQAIRGLTVERGHDLRDFSLAAFGGAG
                     PIFAAFMLMDLEVREVLVPPNAGVFSACGLLMADVRHQFQQPFSAGLDDDHRMDLREQ
                     IRKLHEKAEIALKEDNVLPHKRQVFMSADLRYVGQFHVVRVPFEVTEDSIWDASALKE
                     RFHDLHEKTFGHCNRSGSIESVNLRVEGVGMIDQLDSPGKEKAMDHAPAPVGVRSIVM
                     DRMGTRTNCPVFERDQLLPGHVLVGPAVVRQRDTTIYLLKGQCASVDAQGVISIRMQG
                     "
     gene            56740..58410
                     /locus_tag="VGC69_05700"
     CDS             56740..58410
                     /locus_tag="VGC69_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0294813.1"
                     /translation="MKRVFNSIDRDIFQGQLLGVAEEMSKALRRSAYSPIIWDMYDYS
                     CAILTVDGEILSQAETIPAQLGTMATAFHGVSSEIPLDQWDEGDILICNDPYRGCAHT
                     SDLTLFSPVFNAGELIAIASTIAHHIDVGGSVPGTVSLEHVDLLSEGLVIPPVKLVVA
                     GEISRFVLDLFKSNIRYAEPSLGDLYAQISGCRIAERRITELARRYGNEGFAELATAV
                     LDYGDRYTREMIRKLPDATFEATTLIEDGIVSSDPVILKASLTVSGDSLAIDFTGTSD
                     QRSFGLNCPWASTVSMVMYAVKCVTASEIPSNGGCSRSLKITAPEGSVLNPKWPGAVS
                     ARHLSQQAVADVVLKAFALATPELGSAASQISYPTFRVEGVDDRVGHKRRKYFIADIL
                     GGGAGASAKVDGMDAIDTHGGNCAVLSAEVMETLAPVRVLRSTLVQDSGGMGKFRGGL
                     AMERDYEILSSSAIGAVKLQQLDVNTAPWGFLGGGDGATGFSVLHPDTGLAKNIPFRG
                     ERITYHRGDVIRVRSAGGGGYGNPEQRHQSDHEHDLSEGYVSTHSGSQ"
     gene            complement(58439..59725)
                     /locus_tag="VGC69_05705"
     CDS             complement(58439..59725)
                     /locus_tag="VGC69_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0294814.1"
                     /translation="MTRSNQAHIASRVQNVPQGVVTAHPIFAEKAQGSLLWDVDGRRY
                     IDFVGGIGVQNIGHNHPKVVQAVHKQLERVSHAAFQVAGYDVYVDLAAKLNKLVSGGE
                     PYKTLLVTTGAEAVENAIKIARAYRNVPGIIAFRGGFHGRTLLGSTLTGFSAPYRQNF
                     GPFAGEVYHTPYPDPYRGVSSDNALHALDDLFATQILPERVAAIILEPVQGDGGFLPA
                     GEEFLQALRALTKKHGILLISDEIQAGFGRTGEMFGFQSAGIQPDLVTVAKSLAGGLP
                     LSGVVGRAEIMDAPTPGGLGGTYAGNPLACAAALAVLDVFEEENVLEQGRVLGETLRA
                     ALDKLAAEFKEIGTVRGQGPMLGLEFVRDGDPFQPDADFAQAVIDQCRKDGLLVIKCG
                     VHRNTVRLLAPLNTPLELAQEALAILHGAIRQARKN"
     gene            complement(59722..62712)
                     /locus_tag="VGC69_05710"
     CDS             complement(59722..62712)
                     /locus_tag="VGC69_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEY0294815.1"
                     /translation="MSTALTPGGAKTDLNRLLQLRRRLQSQTTAELRFDAAGRALYAS
                     EASNYRQPPLGVVIPRSMEDLETAVRACGELGIAVLPRGAGTSMCGQAVNDAVCIDHS
                     KHLHAIEQIDPQRRVARVQPGVICDQLKRAAAGHGLTFGPDPATHSRCTLGGMIGNNS
                     CGAHSVMAGKTVENIERLEVLTLSGERFWVGPTDDAEYAAHLTAGGGQARIVRGLKEL
                     AERYANDIRQGFPKLKRRVSGYNLDQLLPENGFNIARALVGSEGTCATVLRAETTLVE
                     GRPERVLVVLGFDTIFDAADSVPDLLPLSAIAMEGLDHGIIGGLRELGLKLDDIAELP
                     EGNAWLLAEFGAASRNEALTQARAAVSVAQSLPSRPSARLVDEAALMNRLWTLRETGA
                     SATSLSRVAGRPDPTVGWEDAAVEPALLGKYLREFSALVKRYGYKTNMYGHFGDGCIH
                     SRITFDLQSKRGVQDWRAFLAEAAALVVKYQGSLSGEHGDGQAKGEFLHLMFTPRLMQ
                     AFREFKALWDPAGLMNPGKLIDAMPVDANLRMGPDYHRSTARGLFTYPMGDGPGTGYG
                     RETERCIGMGKCRSLDGGTMCPSFKATREESYSTRGRARLFFELLNGGVISEDKDARA
                     VKDSMDLCLSCKGCKNDCPTHVDIPKYRSEFLYRYYQNRLRAPMDALIGRLGQWLPAA
                     TRVSGLVNGAMRSPVLRRVGKVFGLAPDTAFPEIAGKAFRRTPTAKRLQRASGEVYGE
                     RDILVWTDTFNNGFTPDVLEAAISVVQSAGYRPRLTSKHVCCGRPFYDSGLLDQARGN
                     LLQILDELAAPIAAGVPVLVLEPSCLSVFRDEMPALLTDDPRAAQLTKLTRTMAEFAR
                     EAGLRLLGATQAHVHTHCHHRACGGAAAEKQLGAKVLDTGCCGMAGAFGYHAKTAEVA
                     RRVGELELLPKLAAVPGEAPLIADGFSCRNQIGKLSGRRALHLAEVLAEQVAHARASN
                     EHSPTDHHAAAGAPRIITDEVSPL"
     gene            complement(62720..64201)
                     /locus_tag="VGC69_05715"
     CDS             complement(62720..64201)
                     /locus_tag="VGC69_05715"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0294816.1"
                     /translation="MPFTFQNSDLLPGLNCIGGAWHAARSGRTLPVTDPATGEVFASV
                     PDSGAADARAALDAAHAAFKTWRKVPAKQRAQIIKRWNDLIVAQIEDLGRLISSEQGK
                     PLAEGKGEVLYAASYVEWFAEEATRADGEIIPAPVAGRRMFALREPVGVVAAITPWNF
                     PAAMIARKIAPALAAGCTVVCKPAEDTPLTSLALVKLAEEAGLPPGVLNIVTASREQA
                     GEVVDVWLDDARVRKITFTGSTPVGKHLARRSADTLKKLSLELGGNAPFIVFEDADLD
                     AAVEGMMAAKFRNGGQTCVCPNRIYVQDAVHDAFVEKLAARVAALKVGPATDPASQIG
                     PMINARAVDKIERHVNDAVSRGAEIVVGGKRISPEGKAGAYYYAPTVMTHVEATMDCS
                     CEETFGPVAPITRFSTEADVIAASNATPFGLAAYFYSQDVRRIWRMADELETGIVGIN
                     EGALAAEAAPFGGVKDSGYGREGSQHGLQEYMHIKYLCQGQLD"
     gene            64387..64884
                     /locus_tag="VGC69_05720"
     CDS             64387..64884
                     /locus_tag="VGC69_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0294817.1"
                     /translation="MFPTPKLDRLDLRILVQLQKNGRMTNVDLAEAVGLSASPCLTRV
                     KRLEEAGYIGGYSAKICMDKLGDVLTVFTEVTLSDHRREDFARFEQAVRDIDEIVECH
                     MVSGGYDYLLKFITRGVNQYQELMESLLERNIGIEKYFSFIVIKSPFIKTHYPIERLF
                     PTGLS"
     gene            complement(64954..66390)
                     /locus_tag="VGC69_05725"
     CDS             complement(64954..66390)
                     /locus_tag="VGC69_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0294818.1"
                     /translation="MPTLTHSPMYSAAQEGFSPMVEWLNGCRYEDVPPDVLTSGKQCL
                     LDGLACLLAGLHAQPIADYRHVLEFNSANKTGLVPIGGALEAGIFDAANLYAQAANIL
                     DFDDCYRYAPSHPGATIIGPALACAHVLKRSGQDMLLAIVKAYEASLRIGASLCPSDA
                     APSKDIGYATWQIFGAYVAAGTLLKLDHDQWIQGFGLAAQQAPVPMIVRTNPQGGYTW
                     LKNTYGAAASAGVMSAFLAKQGYIGDQHFFSEEFGFWKIYGSDRFRPECLDALPGDDW
                     LIRHVEFKPWACCRWSHPALEAILQMKPSIDLRKLQSIDLYAFKEFCGTLDSPYPANL
                     VDAQFNVRFLIAVALLHNDVNAALRAPDFGSEELQKLFSKIHIHHDPECDALRRIDMS
                     VPTKVVVKLAGESSREILISEPLGSERRGIADMAQTQGKFRDALWPLMPGNQAEKIID
                     LVMSLERHTATGLMETAFTEAENVGTTS"
     gene            complement(66681..67565)
                     /locus_tag="VGC69_05730"
     CDS             complement(66681..67565)
                     /locus_tag="VGC69_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294819.1"
                     /translation="MLNFRQIEAFRAVMLSGSMTQAAKDLHTSQPNISRVIGQLERRI
                     SLKLFERHAGKLVPTLEGQVFFRDVEHTFAGLRGLEEAADTIRKRGSGRLRIASVPSM
                     AIVVAPQAISLFARVYPDVDITLQVTDSLTVCQWLATGYVDVGIASEIFNSTSIEHEV
                     LQRNVGVCITPLEHRLATRKRAVTPRDLAEEKFLSLKSSDVMRKQIDEACLIDGEEKR
                     ILAYESHFAAAICHMVSCGMGVSVSNPLVARNYAGRIAILPFSPRIEFPTYLVYPLNS
                     PMNMLAKAFAKALRESVG"
     gene            67726..69195
                     /locus_tag="VGC69_05735"
     CDS             67726..69195
                     /locus_tag="VGC69_05735"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0294820.1"
                     /translation="MLDFAPDEIKLPMGHFIGGKLVGGGPMLDVLRPSDGKRMGGIPD
                     ATPDVVDQAVRNAHEAWRASGWGACPPRERARVLLRWADLLDAHVDELARLEAVGSTR
                     PIREAHAFDVPFTSECIRFFAECADKFGGDAVPTKRDSLGLILPEPYGVVGAIAPWNF
                     PLSMASWKCGPALAAGNAIVLKPSELTPFSILRFAQLAVDAGVPPGVFNVVQGRGAEA
                     GASLVRHPLIGKISFTGSTLTGAAIMSESARHGTKPVTLELGGKSPQLVYDDAGDTAA
                     VAGRIVRGFTSNAGQACVSGTRLIVQRGVAERLIEAIVAQVSGFQPGLTWREATRFPP
                     LINERQAQKVHNAVRDSVAEGATTLCGGDFFPGDLGGYFYRPTLLAGVDSTMRAVREE
                     IFGPVLTVQVFDDEEEGLAMADHPTYGLAAGVHTQDIGRALRATRRIPAGTVWVNRFG
                     RTSDFILPTGGFKGSGLGKDLGRQAFEANLRYKSVLVDF"
     gene            69256..69924
                     /locus_tag="VGC69_05740"
     CDS             69256..69924
                     /locus_tag="VGC69_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY0294821.1"
                     /translation="MAIFKPKYITFDCYGTLINFDMAAAARTQYKGKVSEANMSGFIE
                     SFRGYRLDEVLGAWKPFQAVICNAVERTCRKWGVKYEEADGLAIYNAVPGWGPHPDVP
                     TGLSVVAKEFPLVIFSNAADEQISHNVAKLGAPFHKVFTAEQAQAYKPRYRAFEFMMD
                     SLGCGPQDILHVSSSFRYDLMSAYDLGIKHKAWVNRGHEPASPFYEYTEIKDTSGLAA
                     VVGL"
     gene            69921..71195
                     /locus_tag="VGC69_05745"
     CDS             69921..71195
                     /locus_tag="VGC69_05745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0294822.1"
                     /translation="MKLEPFWLDTAPAFTAGATEALPARADVVVIGAGFTGLSAARTL
                     ARAGVDVVVLESGRIQGEASGRNGGHCSPGTAQGLADLIDKYGLDAAKRYYQTYDDAV
                     DFVERTIVEESIDCDFVRHGKLKLASKAKHFPGLARNHDALRKHLGLSVELLSASDVR
                     RELDSDAFYGGLLDPRGAQMHMGKFGTGLAMAAASHGARIYQHTAVTGLASLGGDRHR
                     VATTQGVIEAGRVLLATGRSDHGPFDWWQRRIVPVGSFVIVTEPIPDLLDQALPQRRN
                     YVTSLNFGNYFRTTADHRLVWGGRARFARSNPASDLKSGKILKASLKSLLPVLKDVRI
                     DYCWGGMVEATADRLPSAGKHEGLYYAVAYSGHGTQMSVYMGDLMADILIGKARVNPW
                     TRENWPALPGYAGRAWFLPLAGVYFRVKDILY"
     gene            71273..72223
                     /locus_tag="VGC69_05750"
     CDS             71273..72223
                     /locus_tag="VGC69_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294823.1"
                     /translation="MNSLILRMIGRRVGVGLITLFIVCALVFFMSNMLPGDAAQMALG
                     QAATPETVAALRQQFGLDQPALLRFFIWLGDLVTGNFGISYAGGVPVKDIVMARIPNS
                     LMLAAAATVTSVPIALVLGIVSAMYRGTRLDRVIGIATMSVVAVPEFLVATLAVLLFA
                     VKLHWMSALSLGTNTSSVTGFFKAYSLPVLVLCCVVVAQMARMTRAALINQLDAAYVE
                     MTVLKGLKPARTVLRHALPNAIGPIVNAMALSLSYLLGGVIIVETIFSYPGLASTLVD
                     AVSNRDLPIVQFCVLFFSACYLLLLLIADIFTIVFNPKLR"
     gene            72226..73080
                     /locus_tag="VGC69_05755"
     CDS             72226..73080
                     /locus_tag="VGC69_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294824.1"
                     /translation="MTRILRFPGACGRFFLSLPIAGRVGLCISTFWIFIAVFGSWIAP
                     YGATDFTDGQMFGPCTLAYPLGTDYLGRDLLSRVLYGARYSIGLALVATLLASTCGTL
                     LALISTVIGRWFDEGLSRLVDALLILPSKILALLMVAIYGSSLPVLVLTAAVTYMPGA
                     YRMARSQAVGLNAMEYVMVSRLNGESKLYIACRDILPNMIHPMMADFGLRFVYNVLML
                     SSLSFLGLGVQPPNADWGSLVRENLSGLSSGAPAIIMPALAIATLTIGANLFIDSLAA
                     RKRLGARG"
     gene            73084..74760
                     /locus_tag="VGC69_05760"
     CDS             73084..74760
                     /locus_tag="VGC69_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294825.1"
                     /translation="MAAQPLVEVSGLQVVARGEDGRETPIVKNVEFSIAKGEVLALIG
                     ESGSGKTTIALALLGYARRGCRIAGGRIRVGDSEIVSASPRDLYALRGSRVAYVAQSA
                     SASFNPSLRIMDQVIEGVCVHGIMDRKAAREKAVDLFRSLALPSPQTIGNRYPHQVSG
                     GQLQRLMAAMALMSDPEVVVFDEPTTALDVTTQIEVLKAFKQAIREHHISAIYVSHDL
                     AVVAQVADKVVVLRNGEIQEQGVLGQIIDAPAHPYTRSLMSAAIPVSRLVDNDAALAA
                     RQPPLLEIKGMTAGYGSLDASGMPTIRILDDIDLVVRRGTTMGVIGESGSGKSTLAYV
                     IAGLLAPAKGQITFAGQPLPGHLVDRDQDLYRRIQLVFQNADKVLNPSHSVGEILARP
                     LKRFERCAPGQITPRVDRLLDMVKLPASIAKRRPAELSGGQKQRINLARALAAEPELI
                     ICDEVTSALDTVVGSAILDLLVELRHALQMSYIFISHDISTVRTTCDEITVLYRGRKV
                     DSCEREALLSQPRHPYTELLIDSVPELRSGWLEEASARLGSRQDEIAAAG"
     gene            complement(74825..76300)
                     /locus_tag="VGC69_05765"
     CDS             complement(74825..76300)
                     /locus_tag="VGC69_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294826.1"
                     /translation="MASLPSFSWADEAPKKGGHVKFAVQNGSVSDTLDPAKGAHSADW
                     TKQFCLFNALTEFNDSLIPDLALAERLESSDGIHWHIALKQGIHFHDGSELTAEDVVY
                     SLARHKDPATASKAFAIASEFTEIKATGKYEVSLVIAKPDFDLPSALGSSYFLIVKNG
                     TKDFTKPVGTGPYILDTFVAGSKFSAKRNPNYWKPGLPHLDSVEIFGVPDNAARVNAV
                     LAGDIDICSLVENRYAKQVKGSSNVELVVNKLALYTDLILRQDNPIAGNQDFVMAVRY
                     MQDRKRMLDNVMQGYGAIANDHPVAPWDPYFMEGLPQRPFDLDKAKYHMQKSGLAGQS
                     IEIICQPGIPASVEGAQFLQGFGAKVGFDFKIKQVPTDGYWSTYWTKFPITYGSISNR
                     PNVGMIFDLFYRSTSTTNEAQWKDPKLDQLLDAARAEGDFTKRKAIYGDMQTLVHESS
                     GTIIPVFTAILDGVSKQIRGYKPNLSGMNMGYRFAESVWRA"
     gene            76622..>78424
                     /locus_tag="VGC69_05770"
     CDS             76622..>78424
                     /locus_tag="VGC69_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0294827.1"
                     /translation="MGLRIGVDIGGSFADFAVLDEADRTIRTLKVFSRPDEPGSEVLA
                     GMSQLAQRYGIDPRDVTYFTHGTTVGVNAVVQRKGLKLGLVTTRNFEDVLDIARLKIP
                     DMYHLKSKRPAPLVTRDRVFGVVERMAADGTVDTPVDEASVLAAAEALRAAGCEGVVV
                     SLLHAYRNPAHEQQVKALIQSAIPGMFVSCSHEVWPIIREYERTLTAVIGGYVQPRVS
                     HYLTSLQGALKQAGVGVDLKVTKSNGGVMSAEYGKNNCVQMILSGTASGVIGAAYVAQ
                     QCGVKRCMSLDIGGTTADMALIIDGQPQYATGEYIGDFQIHIPSVSVSSVGDGGGSIA
                     WVDRFGVLKVGPESAGSNPGPVCYGRGGTQPTITDAFAVMGVIGSAELGYNAVKVDVQ
                     AAREAIAPLAARLGKDVLATAEAIVEVSVSSMYAGVSRIISRFGVDPREFTLMPFGGA
                     GPMMACYLARALNVGKLLVPTTPGVLSALGGLIADLKNDFVKTTYYDLTPATIRALQD
                     EMGRLEAQARAWLAAEGGDPDQAVIDVSADMRYRGQSFEIDTPLKPQWLRDGDMASIS
                     QTFHVEHKRLYGHSDDSAPLQVVALRLVITSRTPK"
BASE COUNT        13593 a        24314 c        25863 g        14654 t
ORIGIN      
        1 gtcgccgatg ctcaccgccc agttgaacgt ggcgttggtg gcgcccgagg cgaggaagat
       61 cagccacagc gcgcgatggc gcagcagttg ccgcagcgag ccgggccgcc acgcgatgat
      121 cgcgagcgcc gacagcacgt agatctgcgc cgtcgcccac agcggatgca ggcccatcgc
      181 ctgcaggtgg cggaacggcc accacgacac gccccacacg aaggcgttga ccagcagggc
      241 gagcaccggt cccgcggagc ggggccgcgg gggcgaggcc gtcatctcaa tggttgtcgc
      301 tgcgcgcgat ctcgaggagc ttcttcaact cctcgggatg gttcctcatg ttgctgcgat
      361 gccagtacat gatcagcagg ctgacgatgg ccgtgaacag gccgaagatc gtgatggagc
      421 cgtagaccga cacgcccatg cgcgtcatgc ccgtgtaaaa cgcgcccagg cccaggatgg
      481 ccgcctgctc gttgaagttc tgcaccgcga tcgaccgccc cgagcccatc aggttgtggc
      541 cgcgatgctg cagcagcgcg ttcatcggca ccaccaggta gccgccgagc gcgcccagca
      601 cgatcaggaa cggcgctgcg atcatcacgc tgtggatcat gttcatcgtc gccaccagca
      661 ggcccatcag gactacttgc tcgggcatgc gtcgccggcc gcgcagctgg cgtggcacgt
      721 ctacgacgac gcctaccatg acttcgactg gccgggcctg cgcacgcatg aagcgcccgc
      781 cttccgcacc cgggcgggcg tggtccccat cgaggggatg aacccggccg cgcgcgagga
      841 tgcgctgcac cgggttccgg aatatctgat gccttaccta ttggagccct gagtctgccc
      901 ggccggctcc ctggctgccc gggcatcggg ctacaggctg aattccgggt cgtgcttcat
      961 caggcgccgg cgcacgctgt cgaggtgggc gcgggcgctg gcggggtctt cggccagcgt
     1021 ctgcgcggcg gtcttggccg cgatggcggg cgggaccggc ttgagccggc ggcccgtggt
     1081 ggcggccagg cgctgcacct cggcggcgcg ttccaggaag tacaggtcgt cccaggccag
     1141 ggcaatcgac ttgcccagca ccatgacacc gtggttcttc atgaagacga tgtcggcctc
     1201 gccgatggcg gccgcgatgc ggtcgccttc ggattcgtcc agggccaggc cgttgtagtc
     1261 gtcgtccacc gccacgcggc cccagaactt cagcgccgtc tggctggccc acagcagcgg
     1321 gtcgccttcg accaggcaca gggcggtggc gttgggcata tgggtgtgga acaccgcgcg
     1381 ggcccgcgga tgctgcttgt gcacccgggc atggatatag aaggcggtgg cctcgggccg
     1441 cccctggccc tgcgccacat tgccgtggaa gtcgcacacc agcaggttgc tggcggtgat
     1501 ttcgtcgaag gccaggccat aggcgttgac caggaagaac tcgtcgctgc cgggcagcat
     1561 ggccgagaag tgattgcaga tgccttcgcc catacccagc cgcgccgcca tgcgcaggct
     1621 ggcggccagg tccaggcggg cttcgcgcac ggcatgcgcg tccagggcgg ccagccgccc
     1681 ggccgcggtg ccgtccaggc cgcgttccgc gttcagggtg tgcgccatgt tctatccccc
     1741 agtcttgttg gctcgatgtc ggatcggcct agtgtagtgg cctctcgggg tcccgtgcgc
     1801 ggcggggagg ccgggcttgc gcgcagcgtt caccgcgtgc ggcgggcagc gtgttatcgg
     1861 tggccgatga ttgcatggtt tgcccggatt cctcggttgc cggccgcgcc ctacattgcc
     1921 cgctgcatgc caagttatat gacattcaag atcatccatg gggaaggccg tgagtaatac
     1981 cgcattggac tcgtcacccg ccgcaacagg ccgccagcgc agtttgaccg tggtgcgcga
     2041 ctgcgtcgcg gcgaccctcg gggcgttttt cgaatggttc gacctgctgg tctacgcctc
     2101 gttcgccctg cctatcgcca aggtcttcta tcacgagggc gatgccgaca aggcctttct
     2161 gctgagcacg ctgaccttcg ccagcgccta cctggcccgc ccgctgggcg cgatcttcct
     2221 gggcatctat tccgacaagc aaggccgccg ggcggcgctg atgctgagcg ccgtgctgat
     2281 gctgatcggc accgccatga tcgcgctgct gcccggctac gacacgctgg gcagcttcgc
     2341 gcccgtgggc ctgctgttcg cgcgcgtgct gcagggcatc gcggcgggcg gcgagttcgg
     2401 cagcgtcaat gcctacctga ccgagcgctt tcccaagctg cgcgccctga tgagcagcct
     2461 gcagttctcg atgacgggcc tggccttgtt ggtcgcgtcc ctggcggcct atgccatcaa
     2521 tcactatctc agccccgaac aagtcatgcg ctggggctgg cggctgcctt tcctctttgg
     2581 ctgcctgatc gcgccggtgg ctttctacat gcgctcgcac atcagcgaga cggaagcctt
     2641 cctggccgag acggccgcgc agcgcgcggc gctgggcagc agcctcggca ggcattggaa
     2701 agccgtcatc ctgggggcgc tggtggtggc gggcggcaat gcggccagct atctcaatat
     2761 cttcatgccc aacttcgcca tcaagaactt cggcctgtcc gcggaagtgg ccttcaccgc
     2821 gggggccttg agcgccgtgg tctgcatcgt cttcccgctg gtcgcggggc gggtcgccga
     2881 ccgcgcgggc ttctccagga ccatgaccat tgcgatgatc gtcggcctga tcggcctggg
     2941 gccggtgtac tacaacctga tgcagcatcc cggcctgccg gcgctggcct tgttccagtg
     3001 cttcatgtcg ctggtcttct acagtttcta ctattcgccg gtgggcgcgc tgctggccga
     3061 cctcttcccc acgcaatgcc gcaccatggg cgtgtcggtg gcctatgtca tctcgcaatc
     3121 gctgttcggc ggactgacgc ccctggtcgt cggcatgctg gtggtcggca cggggtcgaa
     3181 tttcgcggcg gccggctacc tgctggccat cggcgtcgtg gccatcgtcg ccctgcgggt
     3241 gagccgcctg ctgccggcgt acgccgactc gccctgacgg cgggttgttg acctgccaaa
     3301 gcaggccaaa cgcgggagtt ggcagtattt ttctccgctt atttgccgtg ctgcagcaaa
     3361 tcggctcggc tatgttgctg gcacggaccg catcctttgc gccccgactt tccttccgga
     3421 gaaaaaaatg gcaaaaggta tgtccgtgca aatccgcacc cgcatcggcc tgacgctggg
     3481 tttgctgtcg tgcatcatgc tggctctcgg ggtgttcgcg ctgtatgcca ccttccgcgc
     3541 caacagcatc atccaggaag tccacaacag cgacatggcc ggcgcggcgg atatcgccct
     3601 gatccagctg ggcgtgtcgc gccaacgcct ggcgctggac cgcgccgtgc tggtggccgg
     3661 caccccggag gccaccgagc gtgttgccac gctggccaac cgccagaagc agatggacga
     3721 cgcctggcag catttccagt ccctgccgca cgacgccgag gccgcccggc tggccggcgt
     3781 cttcggcacg gcggcgacca atctgcgcaa tgcggtgcaa gccctggcgt cggccgtgcg
     3841 ggccaacgac caggcgcacc tcatcgactt gatgcgcgac ctcacccagc gctatgaaac
     3901 cctgtcgtcc agcgccgacg cgttgaacaa ataccagaac gaccaggcca cgagccgcta
     3961 cgaaggcgcc caggccgcgc atgccatctt ccgcatagtg gccatttcgg ctgtcgtggc
     4021 ggccctgctg ctgacgctct ggtcgtacat cgccctgcgc cgcgccatcg tgcggccgat
     4081 ctcggcggtg ctggcgcaat tgcggcatat tgcctcgagc gacctcagcc accgcgtgcc
     4141 cgcccatgcc gacgacgagc tgggccgcgt gctcgacggc ctgaacgaca tgcaggcccg
     4201 cctgagcgcc accatcagcc aggtgcgcgc cagcggggag tcgatcaacg ccgccacgcg
     4261 cgagatcgcg gcgggcaata tggacctctc ctcgcgcacc gaagagcagg ccgcttcgct
     4321 gcagcagacg gccagcagca tgaacgggct caccggcatc gtgcggcaga acgccgacag
     4381 cgccgaccag gccagcacgc tggccgccag cgcgctgagc acggccggcc agggcaacca
     4441 ggtcatggtc cgcatgaccg acaccatggg caccatcggc cagagttcgc gcgagatcgg
     4501 cgagatcgtc accctgatcg aaaccatttc gttccagacc aacatcctgg cgctgaacgc
     4561 cgcggtggaa gccgcgcgtg ccggcgaaca gggcaagggc tttgccgtgg tggccggcga
     4621 agtgcgcgcc ctggcgcagc gctcggccac ggccgccaag gaaatcaagg cgctcatcgg
     4681 cacctctacc gagcgcgtgc ggcaaggcat ggccttggtg ggcgaggcgt cgggcaccat
     4741 gcaggagatc atcgcttcgg tgcagcgcgt ggccgatctc atgggcgaga tcgccgccgc
     4801 ctcggccgag cagcgccgcg gcatcgagca ggtggcgctg gccgtgacgc agatggatga
     4861 aatgacccag agcaatgccg ccctggtcga gcagagtgcc gcggccgccc agtcgctgga
     4921 cgaacaggcg catcacctgt cgcaggccat tgccgccttc aagacgtcgg aacacgccgt
     4981 gccggccgcg ggccggcaat cggctgcctt gctggaaatg gcgccgtccg cctgagttgc
     5041 gccgccgcgc cgcggcccga agccgttggc ctggcctggc gttgcgtgca gtcgggcagc
     5101 ttgtttatct aacggtatat tattctaata gaatgttata tcattttaaa aacaaccttg
     5161 agccattcgt ccatgaaacg tcacatcaag aagaccgcgc aggccatgct cctgtccgtt
     5221 gccgcggtgg caagcacgca agcctgggcg gcagccgata cgcaaacgac cgtcagcggc
     5281 accgtcacgg actcgaatgg caagccggtg gcgcatgcgg atatcaagct gaccagttcg
     5341 aatggggctg aggccggcca ggccaccacg gatgcggcgg gccgcttcgc ggtggagcat
     5401 gtcacgccgg gcacctacgc ggtcgccgtt tcggcgtccg gcttcgcgcc cacgatgagc
     5461 atggcgcaac ccagcggcga tcacccggcg accgtggccc tgatcctcga gaagaacgac
     5521 gcgctcgatg tccaggtcaa cgccaagcgg ctggaccagg cccgcaacgg cctgctgccg
     5581 gaaaccggca gcagcatctc ccggatcacc caggaggaca tccaggccat gccccagggc
     5641 gaaagcacgc ccttgaacca ggtgctgctc caggcgcccg gggtcgtgaa cgattcctac
     5701 ggccaactgc atgtgcgggg cgaccacgcc gacctgcaat accgcatcaa cggcatcctg
     5761 attccggagc ccatcagcgg cttcgggcaa tcgctcgata cgcgcatcat cgatcaggtc
     5821 aacctcctga ccggcgcgct gccggcggaa tacggctacc gcaccgcggg catcgtggac
     5881 atccgcacca aatcgggcga cctgggcaat ggcggatcga tcgacgtcta tggcgggagc
     5941 caccagaccc tgcagacggg cgccaatgtg tacggcagcg agggcgcttt cagctattac
     6001 ttcggcggtt cggtcgggga gaacaacctc ggcatcgaag cgccgacttc gaattccaat
     6061 ccgctccatg accatacgcg ccagggcagc ggcttcgggt acatgtcgta catgatcgac
     6121 ccgctcaccc gcgtcagcct gatgttcggc acggcgagca accagttcca gttgcccaac
     6181 accccgggcc tcgcgcccaa tttcacgctg gccggcacgc ccacgttcaa ttcggccaac
     6241 ctgaacgaaa cacagtccga gctgaacaac ttcgccgtcg cctcgctgca aggcagcaac
     6301 ggcggcgcgc tggattatca agtggcgttc tttacgcgct acacccgtac ccagttcaac
     6361 cccgatcctc tcggcgacct gatattcaat ggcgtcgcgt ccagcgactt ccacagcgat
     6421 acggcgaacg gcgtgcaggc cgacacgacc tggcggctga acgaccggca cacgatccgc
     6481 acgggggtct ttctccagca ggaacgcgcg agtttcaacg acgatgtctc ggtctttccc
     6541 gtggataccg acggcaacca gacatcgagc gtcccgttca atatcgcgga ctcgagcagc
     6601 aagacgggct atctgtacag cgcctatgcc caggacgaat ggcagatcaa cgacaggctg
     6661 acgttgaatt acggcctgcg ctacgacgga atgaaccagt acgtgcaggc cagccagctc
     6721 agcccccgta tcggcatgtc gttcaagctc acgccgacca cgacgctgca cgcgggctat
     6781 gcgcgctact tcacgccgcc ttccttcgag ctggtcccga attcgacgat caacaatttt
     6841 tccggcacca ccaaccagag ccaggtaacg cagaacgacc cggttcaacc cgagcgcgac
     6901 gactacttcg atatcggcgt ttcgcagcgc ctgggctccg atgtcacggt cggggtcgat
     6961 acctactaca agaaagcgca caacctgctc gacctcggcc agttcggcgc cgccctgatc
     7021 tatgcgccgt tcaactacca gtatgggcgc gtctatggcg ccgagttcac cgcgaactac
     7081 aagcatgacg gcacttcggc ttatatgaac ctcgcgttca gcaaggcgca aggcatgaat
     7141 gtcgactccg cgcagttcaa cttcgacccg gatgaattgg cctacatttc gaatcattgg
     7201 gtgcatctcg accacgacca actcatcacc gcctcgttcg gcggctctta tgcttatcgg
     7261 aataccacct tcaccatgga cgccacggtc ggcagcggct tgcgcgacgg cttcgcgaac
     7321 tccgggaaat tgccgctcta cgcgcagatc aacgtcggcg cgatcgagca catgaacctg
     7381 ccgacgatcg gcaagttcga tgcgcgtctc gtgctgtcca acgtctttgg ccgcacttat
     7441 gaattgcgcg atggaaccgg catcggcgtg caagcccctc agtatggtcc cggccgcgcc
     7501 gtttacgtcg gcatgacaaa atacttctga ttccccattc cggacccgga ttcccatgca
     7561 agcctggctt ggcatcatcg aagcgatacg cctcggcgga tggatcgtct acccgctcac
     7621 gctgctggcg atcgtcgccc tcatcatcat gatggaccgc gcctatgtgc tctggcgctt
     7681 tgccacggtg ccgcatgcgg gcgcgaacgc ggcggccgat atccgcggcg tgctggcggc
     7741 gctgcccgcg cagcacgccg cgcgcagatt gctggcgccg ctggtcgccg acacgactcg
     7801 gccggtctgg tggattgaag aaaaagcccg cgccgtcgcg ctcgaggtgc agcgcgacat
     7861 gtcgcgcggc ctctgggtgc tcgagacgat cgtgacggcc gcgccgttgc tgggcctgct
     7921 cggcacgatc gtcggcatga tgcatgcgtt ccagttgatc ggaaaccagg ggctggtcaa
     7981 tccgaccggc gtgaccgggg gcgtcgcgca ggcgctcgtc gcgacggcca tcggcctggt
     8041 gatcgcgctg gtggcgcttt tcgccttcaa ctatttttcg aggcgcatag accggctggt
     8101 cgaggagctc gaggttttcg ccaacgcgtg gctcagcgac gtgcgcctgc agcgcgaagc
     8161 tgcggagcgg gcggcatgaa actgcgccgc tcgcgagctg ccaagcgcgg ccgcatcgag
     8221 atcattccga tgatcgacgt catgttcttc ctgctggcga cgttcatcct tgcctcgttg
     8281 tccatgcagc ggctcgacgc gctcaagctg gacctgccgc aagggcaggc gcagtccatc
     8341 gccgagcagc agcccctgac cttgagcgtg acccgggacg gccagctcgc gatcaaccgg
     8401 cagccggtca ggctcggcga cgtcgccgcg acggtgaggc ggctggtgca gcccgatggc
     8461 aacatcgtgg tcgcggccga tacggccgcg gcgaacggca tcgtcgtcca ggcgctgctg
     8521 caggcccgac aaggaggggc cgagcacttc atgatcgcgg tcgagcgtgg ccaatagatt
     8581 cgcggatctt gctggcaagt gcttcgacgc gggcggcttc aggacgatcg tcgccggtgt
     8641 cctggcggcg gtggtgtggg gcgtgttcat ggccggcttt gtgcggggga tgatgcctcc
     8701 ggcccagccg gctgcgggcc ccgtcgcgct cgatgcgcgt ttcgtggaac tcgctccccc
     8761 gcctgccttg ccgtctgctg ctccggccac gtccgccgcg ccctctgttc ctgctgagcc
     8821 tcctgcttcc gccgcaccac ccgcaccgcc atccccgcca tccccgccgc cgcctcaggt
     8881 atccaagccg gcgcaaccgg aagcgccggc cgccgcgccg ccgcgcctgg cgcgcgtgcc
     8941 gcagcgatcc acgccgcctg atgcgccccg gcctgcgccc cggcatgacg ggcctcatgc
     9001 cgcccggccg gccgcaatgc cggccagccc ggcgcaggcc gcggcccctt catccccctc
     9061 gacccatgaa gcccccgcgg cggatcctgg cgcctccggg actggcccgg cgcgggccat
     9121 tttgcagcca ttgccggcac tgccggacga tctgcggcaa gaggcgttcc aggcggtcgc
     9181 cagggtgagg ctttcaatcc atcccgatgg ctcggtgagc gtcgaactgc tcgatccgac
     9241 acgcaatccg cgcctgaacc agatactgct caacacgctg gcgaagtggc ggtttttccc
     9301 cgccatgaaa gacggccatg ccatcgaaag cagccaggaa atccgcgttc acttcaatgt
     9361 gaactgagca atgtgaactg agatccgcga tcagtcgcgg gacgtggcat gagccagttg
     9421 cgcggcgagc tcgtcgaaga cgatccgtat gcgtttcggc acgggtccgc gttgcggccg
     9481 atagacatgc agcttccacg gagcgggctc aaagcgcggc aagatgcgcg cgagacgacc
     9541 cgcctcgacg taagggcgcg ctagatattc cgcgcattgg ctaaatccga cgccggcgcg
     9601 ggccacctcg atttccgttt ccggatcatc cgtgacaaac accggattgc gaggctggaa
     9661 ttttgcttcg ccacgaaata tccatggcca gggcctgccg gttgcacggt caatcagggt
     9721 tgtcgtgggc gtggactgca aatcctgcag attccttggc tcccccactc gcgcgatcag
     9781 ctccggcgat cccaccaccc acatcggcag ggggccgatc actcgtgcaa caaagcggtt
     9841 gtcctcgatc gctcctgcgc ggacaccgat atcgatctgc tcgtcgacca ccgccgaagg
     9901 cgtatcggac agccgcacgt caagcctgag gccaggatgg cggatcatca aggcgctcag
     9961 tattggcagc acatagcggc gtccgaatcc ggccggcgcc gtgattcgta cagttcccga
    10021 gacttcgtcc gatgtgctga ttgacgagtc gaacagctcg tcaacggata tcaaggtgga
    10081 ctggaccttg gccgcgaacg tttggccaaa tgccgttatt tgcacccggc gggtgctgcg
    10141 gtgaaacagc gtttcgccca aggcggcctc cagttcacgg atggcccgcg tgacgacctg
    10201 tggggatacg ccaagacgcg tcgcggcttc tcgaaaggtt ggcgagccgg ccgcggcgga
    10261 aaaaattctg agaatctcga gtcgattgag catgatgcgc aggcacttcc ttgaattcca
    10321 taatcaggaa ttatgaatga ataattattc catttatttg tgcttttaca acgctcatac
    10381 ttcgcggcaa gcgcagggcg gcaggcaagc gaaatcctaa tgccgggcgt tctccctcaa
    10441 tgtctgaaca ggaacgaaat catgaatgca agcaccacgc aacgtcagct tgaaggcaag
    10501 gttgccttgg taacgggcgc cagctccggc attggccggg cttcggcgct ggaactggcc
    10561 cgccgcggcg ccaaggtcgt tgtcgccgcc cgccgcaagg acgaactcga ccgggtggtc
    10621 cggcagatcg tcgatgcgga cggcaccgcc gcggccatcg cggtcgacgt ggcaaaagaa
    10681 gaagacatca agaagctggt cggcttcacg gtggcgacct atggccggct tgacgtcgcc
    10741 ctcaacaatg caggtaccga aggggtgttc tcacccttgc tcgaacagga cgcggcccgc
    10801 tacgacatgg tgttcgaacc caatgtgcgc ggcgtcttca attcgatgaa atacgaagcc
    10861 gagttcatgc tcaagcaggg ctccggcagc attatcaaca cggcatcaat gggtgggttg
    10921 atcggtttcg agaacgcgtc ggtgtatatc gccagcaaac acgcggtgat cgggatgacc
    10981 aagaccgcat cgatcgaatg gttccgccgc ggcgtgcgtg tgaatgcgct gtgtcccggc
    11041 ctgatcgaga cgccgtttca tcatcgaggt atttggccgt cggtccaagc gcaacagcag
    11101 tttgcggcgg gcacaccggc cgggcgttgg ggcaccgccg aagagatggc aacgatcgtc
    11161 gcgtttcttg cctccgacga ctcgagctat gtgtccggcc actcgctggt tgccgatggc
    11221 gggtattcga tcgcttgacg ggatcggctc cggatctccc cgcccagggg gatgcgcgtg
    11281 cggcgatgcg cgggaggggc ccttcttgca tatggacatt gcggatttgt cgttgttgga
    11341 ctgctcatcg ccctctcata atcgtcgact tattcaacgt cgacccgcat tgagaaacaa
    11401 cgatgagcat caatcatgat cggcggaaac tgctgaccct tgccggcggc ctggccgtat
    11461 ccggcgcgct gccttcgatc gcgcaggcgg cgcccgctgc ggcccgggtc gtgctgggcg
    11521 tgacgcctct tccgccttac tacgacatca tcgagctgat ccagcccaag gccgccgagc
    11581 tgggcctgga cctggtggtg aagaagttcg aggatacccg ccagctcaac acgctgctgg
    11641 ccaatggcga cctggacgcc aatttcttcc agcatgttcc ttatctggag caatacaaca
    11701 aggacaataa tctcgacctg gtggtggccg cgggcgtctt catcggactg ttcggggctt
    11761 attcgaggcg tttcaagaaa gtcgaggacc tgccggaagg cgcgctgctc gtctatccca
    11821 acgatccgtc gaacagcggc cgcgccctgg acatgttcgc caagcacagg ctgctgcgcc
    11881 tcaaggacgg cgcgggtatc aaggcaaccc aggccgacat catcgacaat cccaagaaat
    11941 tccgcttccg cggcgtcgag cagcccatgc tggtgcgcac gctggacgac gcggacctgg
    12001 tcgccatcaa cgccccgttc gtggtcgcga tcggcctgaa cccgctcgtc gatgcgctga
    12061 tctccgagga caagagcgtc ttcgtcagca gcctggtctc gcggcgggac aatgtgaaga
    12121 gcgacgggat cgtccgcctt gcccaggcgg tgacgtctcc cgaggtcaag cgcctgctcg
    12181 aggacaagtt ccagcgttcc gtccgtccgg tcttttgatc ccgaatgatg ttcgggatgc
    12241 gtgtttcgag gggcgaggcg tgatcgagtt gagccatgtt tccaagcgct tccaaacggc
    12301 cgatggagac gtcgacgcgg tgtccgatgt gtcgttgcgc atcgggcagg gcgagatatt
    12361 cggcatcgtc ggattcagcg gcgcgggcaa gtcgacgctg ctgcggatga tcaacctgct
    12421 ggaacgtccc acgtcggggc gtgtcgtggt cgggggggcg gatttgctcg cgctcccgcc
    12481 ggacggcctg cgccaggcgc gccagtcgat cggcatgatt ttccagcact tcaacctcgt
    12541 ggccaaccgg acggtcgagg acaatgtgtc gatcgccctg gagatcgccc gggtcccgca
    12601 agaccgccgc ggcgacatcg tgcacgagag cctgcggacg gtaggcctcg cggacaaggc
    12661 caggcactac ccggggcagc tcagcggcgg ccagaagcag cgggtggcca ttgcgcgggc
    12721 cattgccaac cggcccaagg tactgctgtg cgacgagcct acttcggcgc tggacccgca
    12781 gacgacgggg gaaatcctca agtacctgaa ggccgtcaac gacggcgcgg gagtgaccat
    12841 cgtcatcgtc acgcacgagc tgggcgtggt caaccggctc tgccatcgcg tcgccgtcat
    12901 ggaaaagggc gaggtcaggg acgagatcga tttccaggat tccgaccgta cgctttccag
    12961 cgtgctgggc ctcgccattc tcgaagagcg ccgccgcgac aaggactacc gcgatgttta
    13021 cgcatttgta tgagcgtctt cccgagatgc tgcaatccct gtgggagacg gcgacgatgg
    13081 tcggcctcgc ggtgggcgcc gcgatcgtcc tggggctgcc cctggggacg ctgctgtacc
    13141 tgacgcgccg cggcgggctg ctcgagcacg ccacgctgta cacgacgata aacagcctcg
    13201 tcaatctcat tcgttcgttt cccttcctga tcctcctggt ggcgttgatt ccgatcacgc
    13261 gattcgtcct gggcacttcg ctcgggacgg tggcggcgtc ggtgcccctg ggggtcgcgg
    13321 ccatcgccta ctactcccgg ctgtcggagc agtcgctgct ggacgtcgat gcgggcgtca
    13381 tcgaagcggc ccaggcgatg ggggcgaccg tctgggagct gctgttcaag ttcttgttcg
    13441 tcgaggcgct gccggggctg gtgcttgggc tgaccatttc catcatcagc ttcatttcct
    13501 actcgacgct cgctggcgcc gtgggcggcg gcggcatagg ggatttcgcc atccagtatg
    13561 gctattaccg cttcgagacg gacgtcatgg tggcgacgat gatcatcatc gcggtcatcg
    13621 tccaggccgt gcagttcggc ggcagccgcc tgtcccgcca tttcgacaag cgctagtttt
    13681 tgcaggagac tcctcatggc taccgaaacc cgggcgctgc atttcaacct gaacttcatc
    13741 aactccggca cgcatagctc ggcctggcgc tggcccggaa acgatcccag ggccttcatc
    13801 gatcccgact tctatgtggc cgtggcgaag accgccgaac ggggcttttt cgacgcgatt
    13861 ttcttcccgg acaaccccgc gctcacgcaa gagccgaggt tccggccatt caacgcgctc
    13921 gagcccacgc tgctgctggc gacgatcgcc gcccgcaccg gccatatcgg cctgatcgcc
    13981 acgatgtcga gcacgctcaa cgagccctac aacgtggcgc gccgctttgc ctcgctggac
    14041 atcctcagca acggccgggc ggggtggaac gtcgtcacga cggcggaccg gggcgcgtcg
    14101 gccaatttcg gcctgcagga cacgctggcg catgccaggc gctacgcgcg ggcggcggaa
    14161 ttcacccacg tggtgaaatc cttgtgggac agctgggaag acgatgccct ggtggcggat
    14221 cccgattccg gcgcctttat cgacacccgc aagatctatc ccttgaacca tcacggagag
    14281 ttcttcgatg tggcgggggc gctgaacctg ccccgtccgc cgcaggggcg gcctatcgtg
    14341 gtgcaggccg gaggctccaa tgacggccgg gacctggccg cggcgcacgc cgacgcggtg
    14401 ttcacgctgg cctatacgct ggaagaggcg gtcgcctata cccaggatat cgatgcccgc
    14461 gcgcgccgat tcggccggac ccgcgacgac atccggatat tcccgggcct ggtgacggtg
    14521 atcggcggca ccgaggcgga ggtccggcag cggctcgagg tacacaagga gctctcgtcg
    14581 ttcgaatacg ggctggcccg gcttgcgggc tcgctgcgca tcgaccccaa gtcgctggcc
    14641 ctggacgaac ccctgcccaa ggacctggcg ctccatcctg atcacaacca cacctttttc
    14701 aagggcatcg tcgaactggc cgcagccaag ggcgggctga cggtgcgcga gctggtctac
    14761 caccagctgg gcgggaccag ccaccggatc atcgccggca cgcccgagca ggtggcggac
    14821 gatatcgaga catggctacg gtcaggcgcc atcgacggct tcaacgtgat gccggacgtg
    14881 ttgccttcgg ggctggacgc tttcgtcaat cacgtcattc ccgagctgcg ccggcgaggc
    14941 ttgtaccggg acggttatcc atcccacacc ttgcgcggca atcttgggct ggatcgtccg
    15001 gccaacccgc gcgccggtca gccctccctg gccgagcagg agcacgtggc ggggcagttc
    15061 ttctagccca agggccgcac gctgccggaa acccggatgc tcgcgccggg ctcgggcgtg
    15121 atttccacgc gcagccggct cggcatgccc atgtccgact ttcgccgcaa tggcctgcat
    15181 ggtggcgcaa tccggcaagg cctggcagat gacgacgcct gcgggattgc cgcccgcgtc
    15241 gccgtcggaa aacgcggcca gccggtctac ggtgagttcg tgtgtctgtg gcatgggtgt
    15301 caaggtgtag tgggcggtgg cgcgggcgtg cggcccgcga cttggcacgc cacacgttgc
    15361 atggggggtg aaaagagggt tataaatgat aactattctt atccattgag atcaggctca
    15421 tcaggaaaga gcctggctcg taccggccgc cgaacttccc gtgcctaggc taggcacacg
    15481 cgctacagcg accgccacgg ccggctgcgc ggctgtatgc gtcgcgcggg ccgcagatta
    15541 atcatcctaa aggctagtgg aactccctgc tagaacaata ctcatggatt ccgatcaaat
    15601 agatgccccg gctgggggcg gcaggccgtg gatccgcggg cgccaagtcc ttggcgatgg
    15661 ggcaatcagc gcgcggtttc gccaggcgtt atggtttgtt ctcttgaccc ttgtgtggct
    15721 tgcgatcacc gcgcccttgt tcatcctcgc actgcaggat ccgctgtcgt gaatgtcagt
    15781 acgccatctg caccatgctc gagaaatcgc agttctcgat ggccagttgc gtaaccgaag
    15841 tcaccagggc gctgcgccgg tcgcgcttgt aggccgccac gtagaccgac ggaggaagcg
    15901 gctgggtggc cctcacaacg tcgagcgtgc cggcgcgcag caatggttcc atgctcttca
    15961 agggtaggta gctcaagccc agcccggaca ccgtcaaggc aatacgcccc accaagctat
    16021 tgcagacgat gaggcgcgcc ggacgcacgc ccatagtgcg cagccaacgc ttgtatacca
    16081 gtccagtacc cgcggtcagc ccctggatga tcagcttgtg gtcggccagc tcattgagct
    16141 tgatcggcgt gccgcgagcc accatgccgg gtttgcacat ccaggcaagc tcgaccttgc
    16201 cgatcggctc gaaattcaat tgcgcgtcct catggtcgcg aaagacgtcc ggcacgatca
    16261 tgagatcgac ttcatcagcc agcagttttt cacgcaggtt gacgctgata tcgatatggg
    16321 gctcgatcgc cacgcgggga tattgcgcct ggaatgccat cgccaagcgg ggcagccagg
    16381 tcatggccgt gagctcggtg actccgatgc gaatctcggt ttccagcacg ctggcctgtg
    16441 cgaaccgttc gacggcttca tcgcgctgca gcagcagttg ctgcgccagc ctgaacatct
    16501 gctcaccccg gggagtcagc cgcgcattac gggaagtgcg gtcgaaaagt tccgtatcga
    16561 aacgacgttc caattcctgt atgcgttttg aaatggcgga ctgcgtggtg tgcagctcct
    16621 gggctgcctg ggcaaaaccg cccgcacgcg tcacccaata gatagcttcg agctgtttga
    16681 aagtgatcac gacatagtcc tcgaataaag agggctgcca ggagggctgc ctgatttaat
    16741 cgctttttgg aatgctattt gatagaaata aatcgctttt cttattttct cgcaaattac
    16801 agaatgcctc caattcaaat cccccgggaa cacccgggga aacaacaagg agacaatgtg
    16861 aaaagcattt cgcgaagctt ggcgctcgtc ctgttcgccg tcgtggcgtt gaacgcacgc
    16921 gctgccggtt atcccgacca gccgttgcgc ttgatcgcgc cctacgcgcc gggcggcaac
    16981 atcgacagca ccgcccgtct gctggcacag gggttgtcgc aacggctggg ccagcctgtc
    17041 attgtcgaaa accgccccgg cgccgcaggc gtggtgggtt cggaagtcgt cgccaagtcg
    17101 aagcccgacg gctataccat ggtgctgaac tccaccggcg cgctggcctc ggcgaaagtt
    17161 ctcaaccctg gcctgaagct ggacccgctg aaggattttg ccgcggccgc cacggtgtcg
    17221 caggcgccga tggcgctcat cgtcaatccg gctctgccgg tgcgcagcgt ggcggaactg
    17281 atcgactacg cgcgcaagcg gccgggcaag ctcaccatgg cctcctccgg catcggtacg
    17341 tcggcccatc tggccggcga aatgttccag caaatgacag gcgtcaagtt cctgcatgtc
    17401 ccgtacgcgg gcagcagcca ggccgtgtcc gacctggtgg cgcagcgcgt cgatctgact
    17461 ttcgaccagc tcacgtcgac gctgcccctg atcaaggccg gcaaattgcg tgccctggcc
    17521 gtcaccacga gcacacgcag catcttcatg cccgaacttc ccacgctgga agagtccggc
    17581 gtgcacggct tcgagatgag cacaacgacg ggcctgctgc tggccaagga cacgccaccc
    17641 gccatcctgg accggctcaa ccgcgacagt gcggcgatcc tgaacgatcc cgccgtcaag
    17701 gacaagatgc tgctcttggg cgcggctccg ctgcccggtt ccggtaatga cttcgaccgg
    17761 atgctgcgtg gcgaaatcga aaaatggacg cgcgtggccg acacggccga tatcaaactg
    17821 ccctgagact agcgaagaga ccccaatatg cagcaagaca ccaacacgca gggcccgttg
    17881 gccggtattc gtgtactgga actgggaacc atgatcgcgg gcccgttctc ggcgacgctc
    17941 ctggccgatt tcggcgccga agtcatcaag gtcgaacagc cgggcgtggg cgaccccatg
    18001 cgtaatatga gcccattcgt caaagacgag agcctatggt ggaacgtcga agggcgtggc
    18061 aagcgctcga tcacggtcga cctgcatgtc gaagacggcc aggaactggt ccgcgacctg
    18121 gcggtgcact gcgaccttct ggtcgagaac ttcaagcccg gaacgatggc gcgctgggga
    18181 ctagggttcg agcgtttgca cgacctgaat cggcgcctgg tgatgatgag cgtctccggc
    18241 tacggccaga ccggccccta tgccggcaag cgctcgtatg accgcatagc ccaggctttt
    18301 gccggcatgt tgaacgcaac tggctttccg gatcgaccgc cggtgcggcc cggcttttcc
    18361 atcgccgact tcggcacggc ggtctttggg gcatttgcgg ccatgatggc gctctaccac
    18421 cgcgacgcgc gcggcggcca agggcagcat atcgatgtcg ccatgtatga agccatgttc
    18481 cggctaaccg agagcatggc catctcgtac gaccagcttg gcgccgtgcg ccagcgccgc
    18541 ggcaacctga acgccggcgc ggccccggga gaccacttcc tgacggctga cgaccgctac
    18601 ctggtcttga ctatctccaa caacgcgctc ttcggccgcc tttgcgaagt catgggccag
    18661 cccgatctgc cacgcgatcc acgctttgcc acgcatgccg agcgggcggc gtaccttccc
    18721 gagatcaatg gcatcgtagg agactggttg cgcaccctga ccgcgcaaca ggccggcgag
    18781 ctgctcgacg cggcgcaact gccgtactcg ctgatctaca acattgcgga catctacgcg
    18841 gacccgcact acgcctcgcg aggcgccatc gccaccgtgg accatcctcg gctggggaaa
    18901 ttgcgcatgc aaggcatcgt gccacgcctg ctgggcacgc ccgcccctgc gctgcgcccc
    18961 gctcccacac tgggccagga tacccgtgat gtgctgacga gcctgctggg aatgacgcaa
    19021 gcgcagatcg agcaactgta tgcgcagaaa atcatctgag gaaacaaaag tcatgggtcc
    19081 gctcgccggg gtgaaggttc ttgaaatgga ggcattggga cccgttcctt gggcggccat
    19141 gatgctctcc gacatgggag cagacgtgct gcgcatcgac cgccctgatg gcggccggcc
    19201 ggatccgtta ggcccgcgca gcgggcaggt catgctgcgc gggcgccgct cgctggtgct
    19261 cgatctcaag cagccgggcg gcggcgaagc ggtcctggag ctggccgcgc gtgccgacat
    19321 cctgcttgag ggcctgcgcc ccggtgtcat ggagcgcctg ggcatcggcc ccgagatctg
    19381 cctggcccgc aatcccgccc tggtctacgg ccgtatgacc ggttggggac agcatgggcc
    19441 gctggcccag caagccggcc acgacatcaa ctacatcgcc gctgcgggcg ttctgcacac
    19501 catcggcccg cacgacggtc caccggcagt gccgctgaac ctgattggcg atcttggcgg
    19561 cggcggcatg ctgctggcgc tcggcgtcgt tagcgcactg ctgcacgccc gggcatgcgg
    19621 cgagggccaa gtggtggatg cggcgatgat agacggctgc aatgcgttga tggcctcggt
    19681 ttgggggcgt ttcgccgcgg gagcgtggtg cgacgagcgc gaacgcaatg tgctcgatgg
    19741 cggcgctccc ttctatagcg tctatgaagc ggcggatggg aaatggttgg ccttcggcgc
    19801 catcgagccg catttctatc aagcgctgct cgatggcctc gggctgtccg gtgaaacctt
    19861 gcccgcccag catgaccgca ccgcctggcc cgacatgcgg cggcgtattg ccggcatcgt
    19921 caggcagcgc agccgcgatg actggtgcgc aatcttcgct ggttcagatg cctgcgtcac
    19981 gccagtgctt tcgctggccg aggcgcgcga gtcggcccat caacgcgcgc gcgatgcctt
    20041 tgtggccatc gagggcgtca tgcaccccgc tccggctccc cgcttctcgc gcagcgccgg
    20101 cgccatcgtc cggccgccgt gcgcgcgtgg cgcgggcggg gcagaggcgc tgcgcgattg
    20161 gggcttggat ccttcccatc cagcgctgcg ccaggtctgg aaccaacccc cctctggcag
    20221 ggaatcctga gccaccggtt gccggtgcgc ctggccttgc gctgtcgcgt tgggtccagt
    20281 ccatcccgcg ctcaggtccg gcagccgcat cgatagggtg gactcgcgtc tgcggcgtgc
    20341 gacatgcagg gctatccctc agccgggata tcggagtcgt taagcagcca ggcccggaaa
    20401 gccacgagcg acggcagcga tgtattgcgc ggcggataga cgaggtagta gctgcgccgg
    20461 ctcgttacgg cgttgcccac catgaccaac tggccctgct gcagttcgtc ttccactagt
    20521 acccggggga ccagtccgat gccgatgtcc gccatcacgg cttgtatcag gtgcgatgtc
    20581 agttcaaagc tggggccaat gcgcatgccg cggggtgtca ggccgtgatg cccgagccac
    20641 tcgttccagc cctgggggtg ggtagcgaca ctcagcagca gatgcgagcc caggctttcc
    20701 tgcgtcaacc gcagtccggc gtcgatggcc gacggactgg cgatcacaac ctgttgctcc
    20761 gtgtgcagcc ggtgcgcagt aaggcctggc cagtcatcgc tgccgacgcc aatggcggcg
    20821 tcgatgccgc tggtgttgaa atcgacggcg cgtatccggg aatgcaggtg gatgagcatg
    20881 ccgggatgcg tggagtagaa gtgatgcagc ctcggcagca gccatttcga accgaacgtg
    20941 ggcagcgtgg caagccgcag cgtgcccctg cccgagcgca aggcaatggc ttgcagcgtg
    21001 gcgccgcgta tctggttcag cgcgggcgag agctcctgca ggtacaagcg gccggcgtcg
    21061 gtcagtgccg cattccggcc atcgcggcgg aacagttcga cgcctagcag ccgctccagc
    21121 gcctggatct gccggctgac ggcaccctgg gtaaggaaca gttcatcggc agcacggctg
    21181 tggctttcgt gtcgcgcggc ggcttcgaag gctagcaatt gcgacatgga ggggacgagt
    21241 tggcgtggat tcatgcgtaa agatcatgat tgaggggaat aatattcgct tgtcggaaag
    21301 ttgtgtgtta ctgaagaata gcagtaagca tgatttattt gccatgatcc acaggcggga
    21361 attttttctg cttgaatcaa ggatcatgca tttaatacat gaatccgggg cggcgtcgct
    21421 ctgggtcttg ctttcttttt ggctttttcc gcatgatttc ccgactcgct cccgctgttt
    21481 ctcccagcca gccgtaccgt gtgtttctcg atgcgttggc cgccagcggc tttcgcggcg
    21541 aaatcgacgc cggcgcagcg atgcgcaccg tgctggccac cgacaattcc atctaccagc
    21601 gtttgcccca agccgccgtt tttccccgcg atgccgagga cgtgcagtcc atcgcccgcc
    21661 tgctggcttt gcccgcgcat cgacgcgtcg ccgtcgcgcc gcgcggcggc ggcaccggca
    21721 ccaacgggca atcgctgacc gacggcatcg tggtcgacct gtcgcgtcac atgaaccgaa
    21781 tcctggagat cgatcctgct gcgcgcaggg tgcgggtgca ggccggcgtc gtcaaggatc
    21841 agctcaacgc ggcgctccgg ccgcacgggc tgttcttcgc gcctgaattg tccacctcca
    21901 accgggcaac gatcggcggc atggtcaata ccgatgccag cgggcagggc agttgtacct
    21961 atggcaagac gcgccaccac gtgctcgcgc tcgattgcgt gttgctcggc ggcgagcggc
    22021 tggccagccg tccggtggcc gagggcgagc tggccgcgct gtgcgccggc gcgggccgcg
    22081 ccggccaggt ctaccgcacc gcacagcgta tccatgccga acgggcggac gagatcgcgg
    22141 cgcagttccc gcgcttgaac cgcagcttga ccggctatga cctggcgcat ttgcgcgaac
    22201 cggacggccg cttcaacctg aacagcgtgt tgtgcggttc ggaggggtcg ctggccatgg
    22261 tggtggaagc cacgttgaac gtgctgccga ttccccgcca cacggtgctg gtcaacgtgc
    22321 gctacggcag tttcatggac gcgctgcgtg acgcccaggc gctgatggcc caccgcccgc
    22381 tgtcgatcga gacggtcgat tccaaagtcc tgatgctggc aatgcaggac attgtctggc
    22441 agggcgtggc gcagtatttt cccgaggacg gcgcgcaacc cgtgcgcggc atcaacctgg
    22501 tggaattctg cggcgacgat ccggccgagt tgcgctggcg caccgaggat ttcctggccc
    22561 atctgcgggc cgaccacagc gtgctgcgcc tgggccatac gttggccgag ggtcatgccg
    22621 cggtcgagcg cgtctacggc atgcgcaagc gcgccgtggg cctgctgggc aatacgcccg
    22681 gggagcagcg tccgcagccc ttcgtcgaag acacggtggt gccgcccggg catctggccg
    22741 attacattgc cgaattccgc gcgctgctcg acggccatgg gctggaatac gggatgttcg
    22801 gccacgtgga cgccggcgtg ctgcacgtac ggccggccct ggacatgaag gatccgcggc
    22861 aggccgccct ggtgcggccg atttcggacg ccgtggccgg cctgacgcgc aagtacggcg
    22921 ggctgctctg gggcgaacac ggcaagggcg tgcgttcgga atacgtgccc gacttcttcg
    22981 gtccgctgta tcccgccttg caggcgctga aagcggcttt cgatcccgcc aaccagctca
    23041 accccggcaa gatcgcgacg ccggccgacg gcggtgcggc cctgctcagg atagacggcg
    23101 tgccgctgcg cggcgaagcc gacaggcaga ttacgccggg cgtctggctg agctacggct
    23161 cggccatgca ctgcaatggc aacggcgcct gctacaactt cgacccggac gatgcgatgt
    23221 gcccgtcctg gaaggccacg cgcgaccgtg tccattcccc caaaggccgg gcctcgctga
    23281 tgcgcgaatg gttgcgccag cagcaggccg ccggcgccga cgtgctggcg ccgcgccggg
    23341 gctggctggc cggactgccg gcgcgttggc gcaacgactg ggccaggcgc cgtggcgagg
    23401 cggatttctc ccatgaggtc tacgatgcga tggccggctg cctggcctgc aaatcctgcg
    23461 cggggcagtg cccggtgaag gtcaatgtgc cggcgctgcg cgcccgcttc ctggcgctgt
    23521 accacacccg ctatccgcgc ccgctgaagg actacgtgat cggttcgatg gagtacaccg
    23581 tcgccggtct gtcgcgcctg ccgcgcctct acaatgcgat catgggcgct gcgccagtgc
    23641 gcgccgctct ggcgcgccgt gtcggcctgg tcgacagtcc gctgttcagc gatcttgact
    23701 ggcgcggcca tctgcgccgg ctgggcgtga gaccggccac tgtgcggcgg ctcgctgcct
    23761 tgaccgaggc acagcgcgcg cgcagcgtgg tgctggtgca ggatgccttc acccgctatt
    23821 tcgagacgcc ggtggcggct gatttcgtcg agttggcggt gcgcgccggc tacgacgtat
    23881 acgtagcgcc tttcctgccc aacggcaagc ctctgcaggt gcacggcttt ctggatggct
    23941 tcgagcgagc ggcccggcgc aacgcggtgc ggctgcgtcg attggccgac tgcggcgttg
    24001 cgctggtcgg cctggatccg gccatgacac tgacctaccg gcaggagtac ccgcaagtgc
    24061 tgggcgagga atcctgcccg cgcgtgctgc tgccgcagga atggttggcc caagcgctgc
    24121 cggcgcgcgg cggccggccg gccgccgccc tatcctaccg gctgctggcg cactgcaccg
    24181 agcgcaccac ggcgccggcc agcgcggccc aatggcgagc ggtcttcgag cgggccgggt
    24241 tgtcgctggc gctgcaggcc agcggctgct gcggcatgtc cggaacctat gggcacgagg
    24301 tccgccacct ggagacctcg caggccattt tcgcgcagtc ctggagcgct ttcgtcgatg
    24361 ccgcgagcga ggacggcgag gatgagctgc tggccacggg atattcctgc cgcagccagg
    24421 tgcggcggct gcgcggccgc gcgctgcgtc acccggtgca agtgctgctg gactattact
    24481 gatcggtgcc tgggtggcgc cgcctcgtgc ctcgtggcca ggccgcgccg cccgaatgcg
    24541 ccgtcaggcg gtcgccagcg tgcccagcag ggcttccatg tcttccttgc gcaccttggg
    24601 ctgccaggga cgccacggca tgcgcagggt ccgggcctgg cgttcctcat aagccaggaa
    24661 ctcctgcgtg atcgcgatac cgcgcacacc gccacggacc gcgccggcac gcacgtcgcc
    24721 gtagtactcc tcccagtcgg ccggcaagcg ctgcgcggac ggcaccgcca gccacaggcg
    24781 cagcaggtgg cgcttgaggt ccggttcgtc gtagtcctcg aaggcggtgc gcgagtgcag
    24841 cgtcacatag ttgttgagca actgcaggtc gccgcgctcc agctccatgg tgtggcacag
    24901 cagcgggctg ggcatgagct catccatcag gtccagcgcc tggatctgct gcggcgtcag
    24961 gcgcggcact tcgggatagt cgcgctgcac cgcatcggtg ttcttgcggt tggtgcgggc
    25021 gcagaagtac tcggggtcgc tgccgaagat ggggcagcgg tagtagggcg gctggctcgg
    25081 atcctgcgtg ctctggtagc tatggaacca gggctgttcg agcaccggga tcaggtctgg
    25141 acggcgtcgc tgcacttcgt tgtacagcgc catggagctg atgactttgc tggtgccgcc
    25201 cgccttggcg gtgcgccggc acagcagggc cacgacatcg caggagtcct ggtggaagtc
    25261 caggccggcg ttggtgttgt agccgcgccc gcccttgacc ttgtagtcca ggccttcgtt
    25321 gcgcacgtcg ttgatgacct ggctggcgcg gttctgggtg cggcccacgc ccatgtacag
    25381 gctcatgccc cagtaggcta tccgggtttc ctcttcgttc caggcgtcgg tgggaaggcc
    25441 cttgaccagg cgcatgcccc agcgcccttg ggtgccgtcg atggcgcgcc gtaacgcggc
    25501 ctgcgaggcc tcgggcagag ggtagtcggc ctgttccatc gacagcagcg gcttggcggc
    25561 ggccttggcg tggcgtaacg ccaggtcgaa accttcggct tcctgcggcg tcaggcgctg
    25621 gatccaggaa tcgtcctgcc gcgcttgttc ggcggtccag gcgctcgggc cggcgtgggg
    25681 agtaggggtc atcatgagtc tccaaagtga tttatgtggc catagtctaa gcacttgtgg
    25741 atggttagaa tagtgaacct atttgacaaa acaggtaagg attcttgatg aagatcgaag
    25801 ccttgcacac gctcgaagcc atcctgcaca ccggtacgtt cgcccaggct gcgctggaac
    25861 gcaacgtgac gcgcagcgcg gtgagcatgc aggtcaagca actggagctc tacgtcgggc
    25921 agccactgtt cgaccgctcc ggcctctacg cgcggcccac ctccctggcc agggaactgg
    25981 tctacaccat gcacgatgcg ctggcgcggc tggaaaccct gcgcaagcgg cccagtgtgg
    26041 cggtggaagg gatcgtgcgg ctgggcgtca tcgaatccat gcagcccatc gtgctgccgg
    26101 gaaccctgaa atggctggcc gagcggcatc cgcggctgga gatacgtccg gctcgcgggc
    26161 gcagcagcac gctcatgacg gcggtgaggt caggcgaact ggacgcggcc ctggtggcgc
    26221 agccgccgga aggcgcctcc gcccgtctgc gatggcagcc catgctgagc cgcgaactga
    26281 tgctgctggc cccgcccgag gcgggcgagg cctcggtaca ggcgctgttt cgcctgtacc
    26341 cctggatccg ctacgaccgc aagacggtca gtggatcggt ggcgtcgcat tacgtcgcac
    26401 agcagatcgg cgacgtgcgc ggcgcgctgg aattcgacag cgccacggcc attctggcca
    26461 tggtcagcgc cgggttgggc gtggccgtca tccagctctc ggaaccggcc ctgctgcaga
    26521 gctatccggt gcgcatcgtg cgcctggggc gcaatgcgcc gtcgatacag tattcgctgg
    26581 tctcgcggcg cggggatgaa ggcagccgcg cgctgcaggc gatgcacgac gccatgcagt
    26641 cggcgctggt cgtggcacgg cacccgaagc gcaagggctg agggcccggg cttgtctatg
    26701 ccgggttcgc gggcaggccg gcccagggcc gccgcagggc atcctgcgcc agccaggccg
    26761 ccacgtcgct gtcgtagcgg cgggtcgtgt cgatgtcgtc ccagccgttg atcaggcgca
    26821 ggcgccatac ggggtcgacg tggaaatcga aaccctggcc gccgacctcg atgcggcagg
    26881 cctcgaggtc cacctcggcc tccacgccgc cgggcgcgag cagtgcgaac atcgcttcgg
    26941 cggcggcttc gtcgaccctg gccggaagca ggccgttgtt caccgcattg gaggcgaata
    27001 tgtcgccgtg cgagggagcg atgacgcagc ggatgcccgc gtccgccagc gcatagacgg
    27061 cggcctcgcg cgacgagccg ctgccgaagt tgcggccggc cacgaggatg cgcgcgccgt
    27121 ggttgcgagg ctggttgagc ggaaactgcc cgtcatgggc gccgtcctgc aggcggcgca
    27181 agtcgtggaa caggaagggg gcatacccct cgggcaggtt gcgcggttgc ttcatgaagc
    27241 gcgccggcag cacctggtcg gtgtcgatgt tgatctgcgg cagtgcgcag gccacggcgc
    27301 gcaggcgtac cagcggttcc atcagggcct ccgttgcgtc agttcgcgca tgtcgatcag
    27361 cctgccggcg acggccgccg cggcgaccat ggccggcgac atcaaatgcg tgcgcgatcc
    27421 caggccttgc cggcctttga agttgcggtt ggtggtggag gcgcagcgtt ggccggcatc
    27481 gagcatgtcg ccgttcatgg ccgcgcacat cgaacatccc gagccgcccc attccagccc
    27541 ggcatcgagg aagatgcggt ccaggccttc tgcttcggcc tggcgctgca ccagcgtgga
    27601 gcccggcgac acgagtcccg gcaccttgct gcggcggccg gccagcaccg cggcggccgc
    27661 acgcaggtcc tcgatgcgcg cattggtgca cgagccgatg aagacgcggt ccacggcgat
    27721 atcggtcagg cgcataccgg gcgacagggc catatagtcg atggcgtcgc gcacgtgcgc
    27781 ggcgcgcgcc gggtcgccca gcgcgtccgg gtccggcacg gtgccgttga tgggcaaggc
    27841 gtcctcgggc gtcgtgcccc aggtcacggt gggttctatc tgcgaggtgt cgagcaccag
    27901 ttcgcgttcg aacacggcgt cgtcgtcgcc ggccagttcg agccattgct gcgccgcctg
    27961 gtcgaatgca tcgccagcgg gcgcgaacgg gcggccgcgc aggtagtcca gcgtgaccgc
    28021 gtcgggcgcg accatgccgc agcgcgcgcc cgactcgatc gacatattgc acagcgtcat
    28081 gcggccttcc atcgagagcg cgcgcacggc ggcgccggcg tattcgatga catgtcccac
    28141 ggcgccgtcg gcgcccagcc gcgccacgat ggtgagcgcc atgtctttgg cgcccaggcc
    28201 gggcgccagt tgaccgtcca cgcgcacgcg cagccgcttc ggccgcttct gccagagcgt
    28261 ctgcgtcatg agcacgtggg cgacttcgga ggcgccgatg ccgaaggccc aggcgcccag
    28321 ggcgccgtgg gtggacgtgt ggctgtcgcc ctcgaccacc agcaggcccg gcagcgtcat
    28381 gcccagctcg gggcccacca cgtgcacgat gccttgccac ggatggtgca tgtcatacag
    28441 gcgaatgccg aaatcgcggg cgttcgcggc gagctggtcc atcatgcggg tcagctcggc
    28501 gttggcgatg ggcaggttgc gcccgcgcgt gggcacgtag tggtcggcga tggccaccgt
    28561 gaggtcggga cgggccacgg tttccccgcg ggcgcgcacc ttgttgaagg catggaatga
    28621 gccttcctgc acatagtgcc ggtccaccca taccaggctg gcgccatctt cgcgctgcgc
    28681 gacgacgtgc gaattccaga gcttgtcgaa cagggtctgc ggggtgcggg gcgtcgacat
    28741 cgtgcagcct cacccttgcg gaaagccgta gagttcggct gggttgtcgg tgaagatgcg
    28801 gcggcgtacg gcttggtcgg ggatccagtc gcagaacagg tcgagcagcg ctccgtcgtt
    28861 gggcaccgtg tccgggggaa acagcgccgg gtgcggccag ttggtgccga agacgaggcg
    28921 gtccggccgc gcttgcacca gcgtctccac caacgggcgg atgtccgcat aatcgggtcc
    28981 gccgctgtcg gaacggcgat accaactgga aatcttggcc cagcaatcgt cgcgcgaacg
    29041 caggatgcgc agcatggcct ggaagccggc gttgcctggc ccctcgccgc cacgcacgct
    29101 gcccaggtga tcgaatacca gtggtacggg caggcgaagc agcgtgtctt ccaaggcggc
    29161 gatttcgtcg gcatggtgaa agtgcacctg caggtgccag cccaacggtc gtatgcgcgg
    29221 cgccagcgcg gcaatggcgt cggtgccgcc gtaacccttc acgaatgtgg aaacgcgcac
    29281 gccacgcatg ccgccccgat gcagccggtc cagttcggcc gcgtcgacgt ccggcggcag
    29341 gccggccacc cctcgcgcac ggtgctgtcc cagttcggcg atcaggtcga gcgtgaggct
    29401 attgtcggcg ccgtaaacgc tggcgctgac ctgcaccgtt cgttcgatgc cgagcactgc
    29461 gcacatggcc agataatcgg cccgggttgc ctgcgccggc gtatacgagc gcgcgggggt
    29521 gtaggggtag cgctcgggtt gttcatagat gtggcagtgg cagtccgtcg cgccgggcgg
    29581 cagcgcgtgg cgcggcggcc gcggccgatg atcggccggc aagcatatcg gcggttggga
    29641 ggtgtcagca atatcggtgt tcatgtcagg gctcggtatg caagtcagcg gagtgtgatg
    29701 tgggcgtcct ggataacctt ggtccacttt tccgtttccg tcttgatgat ccggtcaaaa
    29761 tcggagcccg acccggtcag gacatccgaa ccaaggtcgc cgaattgccg ccgcgtctcg
    29821 gccgagttca acacttgctg catggcggtg ttgatcttgt caatgatctg ctgtggcgtg
    29881 ccggcgggca gcagcaagcc ggtggtcgtg ctggattcga agccgggcag gccggactcg
    29941 gccagcgtcg gaatctgcgg gaccacgctg gaacgcgcac ttgtcgtcac gcccagtgcc
    30001 cgcaacttgc cggcattgat ctgcggcagc gtggaggcaa gttggtcgaa cgtgaggtcg
    30061 acatgaccgc caagcagatc ggccacggct tcgctgctgc ccttgtaggg cacgtgcagg
    30121 agtttggtgc cgctggcgct ctggaacagt tcggccgtca aatgcgcagc cgtgccggta
    30181 ccggacgaag ccaccgtcag ctttcccggc tgctgtttcg cataagcgat gaattcagcc
    30241 acgctgtgca ctggcagcga cggcgagacc accagcagca gcggggcgcg ggcaatcggt
    30301 gacgcggcga cgaaatcctt ttcgacattc agcttcaagt ccggattcag cgccttggcc
    30361 ggcgccagag cgccggttga tgcgagcaac atcgtgtagc cgtccgctgg ggaccgggcc
    30421 acgatttcgg accccaaaat gcccgccgcg cctggccggt tgtccacgat gaccgattgt
    30481 ccgaactgtt cggtcaggcc tttggcgatt atgcgcccgg tggcatcgat gttgcccccg
    30541 ggcgggaagg gcacgatcag cctgatggga tggtcggggt atgctgcttg ggcgcaggcg
    30601 gaagccgcgc agagcgcgac gcaggcgaat agacgggcaa agggtttcat gttgtctcct
    30661 attattgttg cgacgccatg cgctgtgcgc cggcggcgtc atgctttaag ccagcgtagc
    30721 ttagccagcg tagctgcggg ccaattcaag cagttcgggc gtgccgatga ggcggttgag
    30781 ttcgttgaaa tcgaacatct ggtccttcat cggctgcgtg gtgccgcatg ccttgaggct
    30841 cgtgtagtag ttttccagtt gccgcgccgc gaaacgggcc gtgccgcccg ggaagatgac
    30901 gatgcggtag ccgcggccgc tcagttcctc cacgctctgg atgggcgtct tgccgccttc
    30961 caccatattg gccagcatcg gcgcacgcga ggagaaaatg ccgcagacgc ggtccatgtc
    31021 ggccgccgaa gagacggctt cgataaagag cgcgtccgcg ccgttggcca gataggcttc
    31081 cgcgcggtcg atcgccgcat ccaggccttc cacggcgacc gcgtcggtgc gcgccagtat
    31141 gagtgtgtcg ggactgtggc gggcatccag ggcggcgcgc aatttgccgc acatttccgc
    31201 tgtcgatacc agtgatttgt tttccaggtg gccgcagcgc ttggggaagg tctggtcctc
    31261 cagttggatc atggcggccc cagcgcgctc aaaatcccgc acggtgcgct ggacgttgat
    31321 cgcattgcca aagcctgtgt cgccgtcgac gatgaccggc gtgcgcacgc ggtcggtaat
    31381 gcgggacagc gtgtcgaccg cctccttggc agtggtcagg ccgacatcgg agcggccgaa
    31441 ctgggtgtag gaaacggctc cccccgagag gtatagggcc tcgaaacccg cgcgttcggc
    31501 gatcagggcc gtcaaggcgt cgtatacgcc gggcgccagg attgccggtg cggtcagcaa
    31561 tcgtgctttg agtgtgtcgg aaggcaagat ttcatccagt gagtatatgt atcacgacaa
    31621 attctaagag cttgtcgatt aaaataaaat caagtaattt ttaaaaaata gtggaattat
    31681 ttttatgaag tgggaagcct ttgccacgct ggatgcggtg ctttgcacgg ggacgctggc
    31741 cggcgcggcc aagagcatga acctcaccgc cagcgcggtc agcatccaga tgaagcagtt
    31801 ggaggccttt atcggtcatc agcttttcga ccgttcgggg ctgcaggtca aaccgctgcc
    31861 gttggcgcac gaggtggcgg ccgtgatgcg ccgggccaag gtcgaattgg acggtctgcg
    31921 caggccttcg agcattctgc tcgagggcac cgtcgatctg gggatcatcg agtcgatgca
    31981 gtcgaggctg ctgcctggcg ttctgcaggc gctacgcatc agccatcctg cgctgcgcgt
    32041 gcacccgcat cggggcaaga gcgcgcaatt gaccaacgcg gtcaaagcgg gcgacctgga
    32101 cgcggcgatt gtggcgcaac ccgagtccgg cggctcttcc cggctgcagt ggtatgcgct
    32161 gtcgcaatac ccgctgtcct tggtcgtgcc gccgaatgct gaagcccaat ccattcccga
    32221 cctgttcaaa cgctacgaat ggatccagta cgacaaggcg actgtcgcgg gccggctggc
    32281 ggcgcgctat atacaccagc acttcaaacc gctgaccagc gggttggagc tggacggcgt
    32341 gcgggcggtg cttgccatgg tgagcgcggg gctcgggcta tcggtgatcc agttgtccga
    32401 atccaacatc gcccagcttt ttcccgtgcg tgtggtggcg ctgcccaacc cgcccatgat
    32461 ccagttctcg ctggtatttc gcgccagcga cgaacacagc cgtgcgctga tcgccatccg
    32521 ggatgccgtg gtgacgtcag tctaagtgca cggctgacga ggcaatcggc gcgcgattcc
    32581 gccaagcatc ggggcgcttc ccgccccttg tctggcttgc ggtcacggcc ccctttcagg
    32641 gcagcgccag gatctcgcgg gccaggagtt ccagcgtgcg cggcgcgctg ccttcggcct
    32701 tgttgttggc cgtgatccac accggctgtc ccatggcccg ggcctcccgg caggcttggg
    32761 ccagcacgcg ccgggtggcg gggtcgggca ccacgatctt gtcgaagggg aaaaagcgcg
    32821 ccttggcggc ctcgtagcgt tggcccgcga gcaggctcca gcgcgccacc aagggcccgg
    32881 gtccggtcgc ggccacggcc tgcatctggc gcgcggcgga tggcatgcgc gcgtggatcg
    32941 ccacgcaata acgcacgccg cgctcggcca gcatgcgcat catgcgcggc gtgacgacat
    33001 cggggtcgcg aaactccacg gcatagaatg cttgctcgtt cgcacgcgag gcgggcaagg
    33061 gcggcagcgc ggccaggaat gccgacaggt gctccacgaa agccgccgtg tcggccagga
    33121 tgtccagcgg caggggtgaa aactggaaca ccagcgggcc cgccttggcg cccaggcccg
    33181 ccacgcaggg cgcgacgaaa ttctcggtcg ccgcagccgc gtcaagaaaa ccggggttgg
    33241 ggccgcaggg cacgccgcgc tccccgcgtt gtaccgcgtc ggtgtaaaga ttcgggcact
    33301 tgatcacgaa gcgaaagcct tcgggaacct ggctcgcatg gcgcgcgtac tgttcctgcg
    33361 tgagcggcgc gtagaaggtg cggtccatgc tcacgctgcg cagcagcgga tgcgccgagt
    33421 aagcaggcag cccttcgcgt gacaacaggg attcgctggc ctccgcgccg tagacgagac
    33481 cgttccagcc gggaaaagac catgaggacg tgcccaggta gacgtccttg ggcaaggccg
    33541 cgcccaattc ccgcagcggc tcgggatgct cggcgggcgg gatggccttg ccgccggcgc
    33601 gccgcgcgcg tggcgcggag gagggcgggg tgggctcgtc gttgaagaag gaagtctggg
    33661 tcacagtcaa aagaaaaagc cgaccttgaa tgtcggtttt tcgcacatat tttgccaagc
    33721 ggcaacttca ggttcgtaca aagccacgat gagcgcacag cgcgatatca gttcctgcgg
    33781 gtagccatcg caaaatgggg tcgccgcaca gttctcccaa acattatggg gagacggcgg
    33841 ttagctacta ctatgcatga tggaaactct ggaaaaggaa accgtcatgg gcgataggac
    33901 tgtcattggt gcccgggagg ccatgcgcgt cttgccggga tgggccgccg cgcaaacacg
    33961 cgatgccatc gagaagacat ttgtcttcgc cgatttcaat gcggcatttg ggttcatggc
    34021 gcgggctgcc ctgttggcgg agcgcctcga ccaccatccg gaatggtcca atgtctataa
    34081 ccgggtgcag gtattgctga cgacgcacga tgcgggcgga gtcacccgcc tggatgtgcg
    34141 gatggcgcag ttcatggatg aggccgccgc gggtatccac ccaacgccgt gacaaaaaag
    34201 cgagcagctg gcgcgttctt tccaaacagc gctttttgcg cttttctttc cactttgcca
    34261 tgaaccccga agacctgaag cacctcgtca cctggaccat gccgttcggc aagtacaagg
    34321 gccgcttgct ggcggacctg cccggccatt atctgacgtg gttcgcccgc gagggttttc
    34381 cagcggggca actgggccgg ctgctggcgc tgatgcatga acttgaccac aatgggctca
    34441 agcccctgct ggatcccttg cgcggcggtg acgcggtgaa gcaggagaac cgagaaagat
    34501 aaacgctttt actcggcagc ctgcgccgcg ctcgccgggt ggccaggcct ggccaaaccg
    34561 agatgttcgc gtagcgtcct gccctcgtag tcttggcgga acaggccgcg cgccaccagg
    34621 atcggcacga cttcgtcgac gaatttatcc agcggttccg gcgcataggg cagcatgatc
    34681 acgaagccgt cggcggcgcc ggccttgaac cagcattcca tgtcgtcggc gatgtcctgg
    34741 gcggtgccgg acagcagccg atggctgctg accgccagca tgtcccggag gtcgcgcacc
    34801 gtcagatggt tgcgttcggc catggagcgc agcaccttgg cgtggcccag catggcggag
    34861 ctcgagggcg gatagtcggg gagcgggccg tccagatcga gcgtgttgat gtcatagccc
    34921 acccggtcgg caaagctgcg gatggtcgag ggcatgtccg ccatggccgc aatctcggca
    34981 atcgtcttgc gcgccgcctc gcgggtctcg cccatcacga tggagacgcc cagcaggact
    35041 ttcacggcat cgcgcggacg gcctacggcc tgcacccgat cgcgcagccg gttggcgaaa
    35101 gccaccgcct cgtccagatc atgctgggcg gcgaaaatcg catccgccgt ccgggcggca
    35161 aagtcgatgc cttgatcgga ggccccggcc tggaacatga ccggatagcc ctgcggcggg
    35221 cgagcgatgt tgagcggccc cttgacgctg aaatgcgggc cggcatggtt gagttcatgc
    35281 agcttggtcc ggtcgaagaa ttcaccgctt ttcttgtcca ggacgagagc cccgtcttcc
    35341 cagctatccc acagaccctt cgccacttcg acatattccg ccgcgatctc gtagcgctgc
    35401 gcgtggggcg ggtgcggcat ggagccgaaa ttcgccgccg cgtccgggct cgaaccggtc
    35461 acgacgttcc agcccacgcg cccgccgctc agatagtcga tcgaggcaaa ggcgcgggcg
    35521 atattgtagg gctcggaata cgtggtggag acggttgcga ccaggccgat atgcgatgtc
    35581 gtcaccgcca gcgccgagat gagggacagc ggctcgaaac gcatcacggt gcctggcgag
    35641 gcgtcggcac cgctgttcac ggcatcggca aagaacacca ggtcgaattt gccgcgctcg
    35701 gccgtcgtca ccacccggtg caccatggaa aaatccaggg tgccgatcct ggcttcgggc
    35761 agccgccatc cgctgcccac gacgccgccc atcaggccca ggtgcatctt cctagacatc
    35821 tttcccgtcc cgcagggtgg aagcgactgc cggattcatt cagatcggcg aatgggtaat
    35881 ctgatcgacc gtcagcgcac tgtcgggaac gccatatttt tccagcagtt tcatgtaggt
    35941 cccatcggcg accatgctga gcaatgcggc ttcgagcgcc tttctcagcg cagtgttctc
    36001 tttcgtcatg acgatacccg aggcggaata gggctcgtcc gccagcgttt ccttggtcat
    36061 ggacatcttc atcttcgggt tgatcctgga gatccattcg ccgaccgcgc ggcccgtgat
    36121 gaatccatcg ccccttccgt tggccacagc catataggtg gcggcgctgt ccggataata
    36181 ggtgaacttg atctccggct tgccctcggc ctggcatttt ttcgacagtg cggtcgccgt
    36241 gtcgatctgc gaactgccct gcgtcagcga cagttccttg ccgcagaggt cgttgatcgt
    36301 cagtccctcg ttgccctgcc gcaccagaag gcccgagtgg ccggtgatgt aaggcacgaa
    36361 atccacggcc ttgcggcgat ccggggtaat ggcgaactga tccacgccga tatcgtagcg
    36421 cccgctctgc acgccgggga tgatggtcgg gaacttcaag tccgcaaact caggctttac
    36481 gcccaggcgc gccgccagcg cgttcacgat gtcgatgtcg aggcccgcgg tctcgcccgc
    36541 ttccgtctta taggcgaagg gcgcccatgc caaagacgtc gccaccttca atacgcccga
    36601 ggcccggatg ccatcaggca aggcggcccg cgcggcctcg accacctgcg cgccggcggg
    36661 ggcagcgagg ccgagggcgg aaacggcccc catgaccagc accatcaggc caagtcccaa
    36721 acgctttttt ccagcggtca tcgtgtgctt cctctcggtg ttggccaccc ttgcggcacg
    36781 agaccgcaag ggatgcgggc gcggcgttac agacgtccgc gcagcgtcgc cgcctcgtat
    36841 tcggtgcgga acaagccgcg cttctgcaat tcgggcacga ccatgcggca gaactgttcg
    36901 taggaggtgg gcatcgtcat cggccgcagg atgaagccgt cgcaggcccc cgactcgaag
    36961 tagtcctgca agatgtcggc caccatcgcg ggcgtgccga tctgcgcctt ggcggtctgg
    37021 cccttggcat aggactcggc gagcgtgacc ccctcctttt cggccttgat gcgcgaaacg
    37081 tccgtcgagc catagatgcc ctgattgccc cgtgcggcga ccacttcctc gaccgtcttg
    37141 tgcttgccca gatccgcccc ggcccccatc gaggcataag cgagatcgga ttccttttcc
    37201 tgaaggctgt cgaggaactc ggcccgctct tgcgcgatgg aataggtctc gcccaggatc
    37261 ggcgtaatcc ccatcaggaa cttgcaagca tcgggatcgc gcccttcttc cgcaatccgc
    37321 cgccgcatat ccgtggtgta ggcgagcatc gtttccttgt cgctacctgg cgcgaagatc
    37381 atctcggccc atcgcgcacc gaaggcgcga ccgcgcggcg agcccccggc ctgcatgatg
    37441 acggggtgcc cctgcgggct gggcggcgta ggcagcggac cgcgcgaagc gatccatttg
    37501 ccttggtaat cggcgtagcg cactttcgcg gggtcggcaa acagacccga ctccttgtcc
    37561 aggacgaagg cgtccggctc ccaggtgtgc cagagggcca tgcaggcttc caggaattca
    37621 tctgcccgct catagcgttc gtcgtgaggg tagatttcgg tcaggccggc gttcttcgct
    37681 tccatgtcgg tcgaggaggt cacgacattc cacgccgctc ggcccttgga cagcatgtcg
    37741 agggaagcca gtgcccgcgc cacctggaag gcattgttga acgtggtgga gatcgtggcg
    37801 cccaggccca ggcgcgtcgt gacccttgcc atcaggggca gcaccgtcat gggatccagc
    37861 cagctgttct ggccgccgtt cttcacatag gtttcgaaag tgccgccgaa gacttcagcg
    37921 atcccgaagg catcggcaaa gaaaacgcca tcgaacttgg cctgttcgaa caggatggcc
    37981 atttgttcgt agcgcgtggg ttcgaaaata tcgttcagtg tggcttcagg gtggcgccag
    38041 ccgccgaaat gcgccacggt cggcccagtc ttgatatagg cgagcagccg catcatgcgc
    38101 ttgcctttcg tttccgccat catgcttttc ctgttgtccg tcccgctgga gcgggagcgc
    38161 gccaatctct gtggataaac cgaatcgctt ccgaaatttg gtcgatcgca cacaccgcgt
    38221 caatagctgt ttccccgatc cagtcgatcg gatggacgat gcggtcgatc ccaggccttc
    38281 cggatcgcct ccatcaccgc ccgcacgggc gcggcgcgtt tgaggtcggt atgcaccacc
    38341 agccagaggt cgcgcgacgc cagcggctgg tctcgagcaa tcaaggccag gcctggatgc
    38401 ccctgcgcca tgaaatcggg cagggcggca atccccgcgc ccgcccgggc agccgccaat
    38461 tggagctcga cgctattggc atggaacgcg aacggcctgt cggccgcata agcttgcagc
    38521 gcctgttgct gcggcgactg gccgagctcg ccgccggaac ccacgaaacg ccagtcggct
    38581 tcccgcacgg cggccaggta ggacgggctg gcgtatagcc tgaaatggat gtcccccagc
    38641 ttgagcaggg tcaggtcgcc gtgttccgga cgggtcatgc gcagggcgat gtcggcctcg
    38701 ctatgcgcca gcgaggccgt gcgggcctcg cccaggatgc ggatggccag ctccgggtgg
    38761 cgctgttgca gggcgaccag cggctgggcc aggcgcgccg cggcaagcgc gggcggcgcg
    38821 ctgatggtga ccgtgcccgc gagttcggcg cgtgcgcctt cggccgatct tttggcggcc
    38881 tgcgcctcgt ggttcatgcg ccgcgcgatg gccgcgatgc gttcgccctc ggcggtcagc
    38941 gcccagcggc ggccgcgcct gtcggccagg actaccccca ggctggcttc cagcgcggcg
    39001 atgcggcggg ccacggtggc gtgttccact cccagcaggc gggcagcggc cgacaaggaa
    39061 ccagcctcgg ccaaggccgc aaagtgatgc aggtcatccc agttcatcgg ttaatttttt
    39121 cacaagcgat gtcaaattat ggggaatttt caatcgaaaa ggaatgcgcg agactgtcgg
    39181 aggatcacca ctactctgga tgaggaatga ccatggacat caaaatcgaa ttgcgcgccc
    39241 agggcggcat cgacaatctg cacgtggcgg attgcccgcc gcaggagccc ggcagcggcg
    39301 aggtgcgcat ccgccaggcg gcggcgggcg tcaatttcat cgacgtatac cagcgccagg
    39361 ggctgtattc cctgtctttg ccgatggtgc tgggcgtgga gggcgcgggc gtcatcgagg
    39421 ccgtgggcgc cggcgtggaa gggttgcgcg tgggcgatcg ggtggcctac ggggccatgc
    39481 tgggcggcta tgcggcaacg cggctactgc ctgcctggcg cgtcgtccgc ctgcctgatg
    39541 cggtcgattt cggcatcgcc gcggcctcgt tcctgcgcgg tatgacagcc cacatgctgt
    39601 tcacccatac ctgttcggtg cagcccggca tgatcctgct gatccatgcg gcggcgggcg
    39661 gcctgggcgc gacgttgacg cgctgggcca agcagcgcgg ctgcgtggtc atcggcacgg
    39721 tgagctcggc cgggaaggcc gaggtcgccc gggcccatgg cgccgaccat ctcatcgtcg
    39781 gccgccacgc agacgtggtg ggcgaggtgg cccggctgac caacgggcgg ggcgtggatt
    39841 tcgccatcga cggcctgggc ggcgacgtgc tgcccaagac gttcgcgtgc atgcgcagct
    39901 tcggcacagt agccagcgtg ggccaggcgg ccgggccgat tgcgccgatt ccggtggagg
    39961 ccctgagctc gccgcgctgc ctgagcctgg cgcggccgag catcatggcg tattgcgccg
    40021 acccggaccg ctaccgggcc gcggcggcgg ccgtcgtcga agccatcggc ctgggcctga
    40081 tgccggcgcc gggccaccgc tttgcgctaa aggacgcggc gcgcgcgcag cacgagctcg
    40141 agtccggcaa gacgaccggc agtatcgtgc tgattccctg accgggtttc ggtgcctagg
    40201 accggccatc atgaaacgaa agcaccttca gcccggttcg ccaggtgatg gagcagagac
    40261 ttgccgactg tcccgcgcgt acaggtacca tctgtgtcat cacataggcc tgaaaggaga
    40321 attcgccatg tttatcgcca tgaaccgttt ccgcatcgcg ctgggccgcg aagaagaatt
    40381 catcgaaata tggcgtcagc gcgacagccg cctggacacc gtgccggggt tcaagaaatt
    40441 caacctgctg cgcgggccgt cggacgacga ggcgaccgtg ttcgtttcac actccacctg
    40501 ggaaagcggc gcggcgttcg aggcctggac gaaatcggag gcttttcgcg ccgcccacgc
    40561 gggtgcgggc ggcagcaagg ggctgtacct cgggcatccg aagttcgagg gtttcgaggc
    40621 ggtgctgtaa gggatggcct ggaagaccta gcggtttccg ggcttgcgaa acttcagcag
    40681 gaagctgtcc gcctcgccga tggcggcgta gcgcgcgcgg tctttgtcgc ccaggcggaa
    40741 ggtgggcggc agggtccata cgccggccgg gtagtccttg gtgtccttgg gattggccaa
    40801 tatttccgaa ccgccggcga actcgaagcc ggcctgttcc atcaggtgga tggcgtagtc
    40861 ttgccggaca tagccgtcca gcgccttcgg gtcctgcggc ttgtcgtcgc ggccccggtg
    40921 ttcttcgatg ccgagcatgc cgcccggctt gagcgcccgg tagaacgccg ccagcgcctg
    40981 cgccgcatag ccgtccgcca tccagttgtg cagattgcgg aaggtcagca ccacgtcggc
    41041 gctgcccggg ggcgcgatgt cgtatttgcc cgggccgaat ggcgtcattc ggaccttgcc
    41101 gtagcgggcg gggtccgcgt ccagcttggc gcggaattgc tgcgtctcgc ggcgctgccc
    41161 ggcatcggcg gcatcggcgg catcgagtgg atacgacgcc gcgtaatagg tgccccgtgc
    41221 ttgcagatag ggtgccagga tctccgtcca atagccgccg cccggcgaaa tctcgaccac
    41281 cgtggcatcc ggccgaacgc caaagaaacg cagttcgtcg tagggcttgc gggccgcgtc
    41341 gcgcgctacg aaggcgggcg tccgctggga ggagtcgact gcctggcgca atgcgccttc
    41401 gtacgaggca tcctggtcgc gaggcacgga tgcgcagccg gcggtcaggg ccaggatgca
    41461 cgccgcgaga gttgcgggaa atttcaggga catgaagggc gatcgccggt tggatgtcat
    41521 gggtcgaatc ttaacggcat tcacccggac cgatgcaaat tcgggcttca tggcggcgga
    41581 tcggcactca ggtgccacca actcggcaac agcgcgcgca ctttgcgctg gcggaagcgg
    41641 tcgtcgatca ggtgcaccgt gccggtgtcg gtcgccgtgc ggatcacgcg cccggccgcc
    41701 tgcaccacct tctgcaatcc gggatagaga taggtgtagt cgtaggcgaa ctgctggcca
    41761 aagcgcgcgc ccatggacgc cagaatctgc tgattgaccg ggtttacctg cggcagtccc
    41821 agcgtggcga tgaaggcgcc gatcaggcgc cgcccgggca gatccacgcc ttcggcgaac
    41881 gcgccgccga gcaccgcgaa gcccacgccg cgcccgtcct cggtaaagcg cgccagaaag
    41941 cttgcgcgtt cgtggtcgtt catgcgcggg gcctgcgtcc acaagggcac gtccggatgg
    42001 gcgcgggcca tgcattcagc cacctgctcc gcgtaggcga agctgctgaa aaagacgaga
    42061 tagttgcctg gctggtcggc gtattgcgcg gccacgagat cggcgatcgg cgccagcgac
    42121 cgcgcccgat catgccagcg cgtggacacc gtggtcacga cgttcacgct cagttgatgc
    42181 gcggaaaacg gcgtgtcgac gtcgacccag tccgtgttcg cgggcagtcc cagcgtatcg
    42241 aggtggaaat cgcgcgggct gagcgtggcg gagaatagca ccgtgcagcg cgcggccgcg
    42301 tggcgctcga ccagataggg ggccggcacc acgttgcgga tgcagatttc cgattcgggc
    42361 ttggccgatg tgccggcggc gtcgcgcacc gcaagatcga acagcgcatg gttgccaaag
    42421 ctcgcggcca ggcggtcgaa ctgcagcagc gtgaagtaca gggcgagcgc gggatcgccg
    42481 ggcgcggcgg gcccgctggc gaagtgctcg gcgagcgccg cgttggcacg ccggacggct
    42541 tcgcatagat tggccggcac caggtcatag gccgcgtacg gcgttgcctg cttcttctgc
    42601 acggcgcgcc aggaacgcaa cagtccatcg agcggcttgc gtagctgggg cggcgcggcc
    42661 atgcgcgccg cgcgcacctg cgccatggac agcgacacgc tgtacatgcc tcgcgcccgc
    42721 tccagcaggt tgtgcgcttc gtccaccgcc accgccacgc gccattgatg cgcctgggtg
    42781 agcgcataca gcatcgcgct ggcgtcgaac cagtagttgt agtcgcccac caccacgtcg
    42841 ctccagcgcg cgagctcctg gcccaggtag taggggcaga ccccgtgttc gtcggccgcg
    42901 gcccgcaccg actcccgcgt cagcgtggcg tccggacgcg ccagggcgtg cgcccgcgcc
    42961 gccggcaggc ggtcgtagaa gccatgggcc cgcgggcagg atgcgccatg gcaggcgcgg
    43021 tcggggtgcg cgcaggcctt gtcgcgcgcg accagttcca gcacgcgcaa gggcaggcct
    43081 gcttcgcgcg tcgcatgcag gcgggcgatg gcgtccagcg ccaggccgtg tcccgtgccc
    43141 ttggcggaca aaaaatacac cttgtcgagc ccctgcgtcg cgcaggcctt gagcagggga
    43201 aagagcgtgc ccagcgtctt gcccacgccg gtgggcgcct gggccaggac gggacggccg
    43261 tcgcgcgcgg cccggtacac cgccaccgca agctcgcgct ggccggcgcg catcttgcca
    43321 tgcggaaagg ccagcgattc gagcgccgcg tcgcgcgcct gccggtgcgc gcgctcgcgc
    43381 gcggcccatt cggcgtagcg ctcgcacagg tcgttgaaga aagcggccag cgcggccgcg
    43441 tccagggttt cggcgacggc ggtctcggcc tcggtgtcca ggttgaaata gaccacggcg
    43501 acggtgatct cgccgaggcc gcgcgacgcg cacagcatat gggcatagac gcgcgcctgc
    43561 gcgcggtgca gcgcgcgctg gttgtcgcgc agggcatcga gatcgccgcg gtaggtcttg
    43621 acctcctcca agcggcgcgt tcccggctcg tagccgtcgg cgcggccacg caccgtcagg
    43681 ccggcatggc tggctgtcag cgcgacctcg tgttcgaagt cctcgccgcg ccgctgggcg
    43741 atcacggcat ggccggccat gccctccagc gcggtgggcg cgggcgtgaa gcgcaagtcc
    43801 aggtcgccgg cgcgcgcggt gaagtcgcac aggccgcgca cggccacttc cagcttcatg
    43861 ccgcctgctc ctcgcccgta gcccagcgca catggcagac ccgtagcggc aagccctgcg
    43921 cggcggcgta ttcgagccag cgcgcctggt tgtcctgcag gcggtcgccc gggcccttga
    43981 cctcgatgaa ctcgtagcgg cgctgcgccg gccagaaccg cacgagatcg ggcaggccgg
    44041 agcgattcgt cttcaggtca tcgagcagcc gcgcgaacca cagcgacagg tgcgccgcgg
    44101 ggatgcagtc cagcgcggtg ccgagcaatt cgcgcgtcag catgccccag aacacgaaag
    44161 gcgactggat gccggctttt gcctcgaagc gcgccaggat gcgcgcgcgg tggccgccgt
    44221 cgccgtcgtc tcgcagggtg gccaggcacg cgtcgaaggc ctgcgcgcgg cgcgcgcgga
    44281 aatcgggctc gtgcaagtcg gcggggcctt gctggaaggg atggaagaac gccccgggca
    44341 cgggctggaa aatggcgtcc cagcacagca ggccgaacag ggagttgacc aggccgttct
    44401 ccacccagtg cacctgcgag ccgtcctcgg cgaagtggtc gcgcgcgacg atctcgaccc
    44461 gggacgctcc gtccgggcac gcaagcgtga gcgtggtggc gtcggccgcg cgccgcgcgc
    44521 gctgcgcggg cgccggctgc ccggcctgcc gcgccaggcg cggcaacata cgggagacct
    44581 gttgccgttc ggcctcgctc gcggcgctgg ccagcgtctg ccgagccagg gtgtgcgcct
    44641 cggcaaagcg ttgctggcgc tccagcacgc ggatcagccg ctggcgcgcg ccggaatagg
    44701 cggtggcgcg ataggcttgc tcggcttcag tccagtcgcc ggcgcgttcg caccactgtc
    44761 ccaggcgaaa ccacagcttg gcctgccggc gcgcgagcca gggcacggcg gtctcgcagg
    44821 cggccaactg cgcgcgcaac gcgggcgcct cgcccggccg ggccgtctcg agactatccc
    44881 gcaccgcctg tagcgcgaga taaacgtcga cgtcggccgc ctgctggaag gcgcgattgg
    44941 cggcatcgat ctcgaccttc tcgtactgga aaatgcccag gtcggcgagc acgaactcgg
    45001 accaggtctg gtgcagattg ccgaagaaca tgaggcgcag ccgctgcgcc agcggcgcca
    45061 cgtcgacgga ccagatcgcc tcgcccggcg cggcgcccca ggcgctgcat gggcgcgcgt
    45121 gcggttcgcc gagaaacgcg gcgagcctgg gcagcagttc cgccttgcgc agggcagggc
    45181 gcagcccggc ctgcggatag cgccgcgtga gttcttcctt ggtgtgcagc gccgcccagt
    45241 cggaccaagc gagcgtcggg tcggcgcgca gccagcccag cgcgagcagg ggctccgcgg
    45301 ccggcgcgac gggaccgatc tcctcgtatt gcagccgttc ggcgcggtac acggcgcaac
    45361 gccgcatcag caggcgcacc agcaatgccc gcgaagccat gggcaggccg gggaaggcgc
    45421 gcaggaagtc cagttcggcc ggggcgagca cgtcggcata ccgcgcggcg agccagtcga
    45481 gcgcgtgctg gaagttatgc aggtaatagt agcggtacgc gggaaacatg ccgggccggg
    45541 cgcgggattc ttcaaggact ggggttttat ccagtgcaat cataaccccc ttgcttctgc
    45601 tcgccggtcg tatcagcact ggcgaaggaa tcgggattca ctatggcctg gggatgttca
    45661 ttttcttgtc atgcttcact gatgatgtgg gtcttcaatg gaagtctcgg ctgctgattg
    45721 ccaattcgcc cagcagggga gtcagatcca ccagcctgcc gaccacgaga tgctgcaccc
    45781 cggacttgga ctgccacgag ccataggcgc ccagcagccg ggcacccagc agctcccggc
    45841 gctggcggtc gacgagctcg ggccgcacga tgagattgac ctggccgtcc tcgtcttcga
    45901 tcgtcacgaa aacgatccca cgcgaagtgc caggacgctg gcgaacggtc acgatcccgg
    45961 cggcgcgtac caggcggcgg ttgggcatat cgcgtagctg cgacgacggg gcgaagcgca
    46021 gcgcggtcaa ctgctcgcgc agcagcgcga tcgggtgccg gcccagtgtc aggcccagat
    46081 gctggtaatc ggccacgagc gcctgccctt cgcgcattcc cggaagctgc gggcgagccg
    46141 tctcgcggat ctcggcttcc cgcagcagcc cgcgctcggg cgcgctcacg atggcgtccc
    46201 acagggcctc acggcgccga ccggccaggg ggcgcagcgc gtcggccgcg gccaaggctt
    46261 gcaggtcgtg acgactaagg tcggcgcgcc gcgccaggtc ggccaccgag gcgaacggat
    46321 gcacggcgcg cgcgacttcg atgcgccatg cggcctcctg gcgcatgcct ttcacctgat
    46381 taatccccag ccgcaccgct ggacgcgcag cgctgcaggc ctcgagctgc gcgtcccagt
    46441 tgctgcgcgt ggcatcgaca ggcaatacca tcacgccgtg acggcgggcg tcctgcacca
    46501 gaatggaggg agaataaaac cccatcggtt ggctattgag cagcgcagcc aggaacgcct
    46561 cgggttcatg gcatttgagc caggcgctgg cgtaggccag cagtgcgaag ctggccgaat
    46621 gcgactcggg aaacccgtat tctccgaagc cctggatctg ctgatagatt tgttcggcaa
    46681 aggcgcggtc gtagccgcgc tcggtcatgc ccacaatgat cttgtcgtag aacttgccca
    46741 ggccgccctt gcgcttccac gccgccatcg agcgtcgcag ttcgtcggct tcccccgccg
    46801 tgaagccggc cgcaagcatg gccacctgca tcacctgctc ctggaagatc ggcacgccaa
    46861 gcgtgcgttc gagcgcctgg cgcatgtcgt cgctcggata ggtcacaggg tcgagctttt
    46921 gccggcgccg cagataggga cggaccatgc cgccttggat tgggccgggg cgcacgatcg
    46981 ccacctggac gacgaggtcg tagtatttgc gcggccgcag gcggggcagc atgctcatct
    47041 gggcgcgcga ctcgatctgg aacgtgccga tcgtgtcggc cgcgcagatc atgtcgtagg
    47101 ttttttcgtc ttcaggcggg atgtcttgca gttcgaatac gtcgccgcgc tgtgcgctga
    47161 cgagatcgag cgcgcggcgc agcacgctca gcatgccgag cgccagcacg tcgaccttga
    47221 gcaggccgac cgcgtccaga tcatccttgt cccattgcac gacgctgcgg cccgccatgg
    47281 cggccggctc gatcgggacc agccggcaca acggcccgcg cgagatgacg aaaccgcccg
    47341 gatgctgcga caggtggcgc ggaaagccca tcaggcgttc ggccagcacg atccactgct
    47401 gcgccaccgg cgactcggga tccagtccgc tctcggccat gcgcgccgcc aggttggcgc
    47461 gcccgtccca gtagtggtgc gacttcgcca ccgcatcgac cagcatcggg tccacgccca
    47521 gcgcgcggcc cgcgtcgcgg atggcgctct tggtccggta gctgatgacc acggcggtca
    47581 gcgcggcccg gtccttgccg tacttttcgt agatgtactg gatgatttct tcgcggcgct
    47641 ggtgctcgaa atcgacatcg atgtcgggcg gctcattgcg ttcgcggctg atgaagcgct
    47701 cgaagaggct ggtgccgcgc gcggggtcca cttccgtaat gcccaggcaa tagcagacgg
    47761 cgctgttcgc cgccgagccg cggccctggc agaggatgcc gcgccggcgc gcgaagctga
    47821 ccaggtcgta gacggtcagg aaataggctt cgtacttcag gtccgcgatc agcgccagct
    47881 cgtgctcgat ctgcgcctgg acttgagggg gcatgccttg ccggaagcgg cggcgcgcac
    47941 cgagatacgt ctcctggcgc aggtgctcgg ccggcgtctt gcccggcggg cagacctcgt
    48001 cggggtattc gtagcgcagt tcgtccaggt tgaatgggca ctgcgtcgcc acttccagcg
    48061 tctgctccag cagctcgcgc ggatacaggt tggccaggcg caggcgcgag cgcagatgct
    48121 gttcggcgtt cgaggcccgc tcgaaaccgc actcggcaat gggagtcctc agccgggtag
    48181 ccgtcagcgt gtcatgcagc ggcttgcgcg aacgcccgtg catttcgacc tggccggtgg
    48241 ccaccagcgg caggtggcag cgccgcgccg cctcgacgac atgggcacgg tggcggtcgt
    48301 cctgcgcgcg gtgcagcaaa gtgagcgcca tccagcaacg ccccgggaaa gtcgccgccg
    48361 cccattcggc ctgcgccaac aatcgttcga gcggcgcgcc gtattcgggg gtaagcagca
    48421 ccacgcattc gggcaggttg cgcagatgcg cgtacgacgg ttcggtgggc tggtccaggt
    48481 ccgagggcat cagccgatat tcgcccttgg aggcccggcc gcgcgccagc gtgatcagct
    48541 cgcacaggtt gccgtagccg cggcgtgtct gcgcgagcgc ggtcagctgg acggccggct
    48601 tgccatcggc attgcgcagc acgaagctcg agccgaccag gaggggaaac ttgtgcttct
    48661 tggcttgcac gtgcgcgcgg accacgccgg caacggagca ttcgtccgtg atcgccagcg
    48721 cggcatagcc gagctcgatc gcacgcgcca ccagctcttc cgggtgcgaa gcgccgcgca
    48781 agaaggtgta gttcgtcgtg cattgcagct cggcatacgc aggtaaggtg gccgccatgg
    48841 catgtcatcc gaacaagcca tggagaaacc agccgcttgc ctcgccgcgc gcgcggaaca
    48901 accagtagcg agcgccgtcc ggggcctcgg cgacgaaata gtcgcgtacg atcaggccgc
    48961 tccaccagcc gtcctcgatg cgctcgggac cctgcacgat acgcagcggc gcgccgtagt
    49021 aggggcggtg gccacgcgtg ctcaatgcga tgggcgactc cagcagccag catggccggg
    49081 tgacgccggg cgcgtagcgt gccggcccgg ccggggcatt gttcggcacc cagcggttgg
    49141 cggtctcggg acgatgatca gccacaggac ttggatgcaa gacgttttcg cgccccaggc
    49201 gcgccaccag cagcgccatg acgtgggcgt gatcggctgt gttgccgcct tgctccggga
    49261 agaggtccat gggcacgggc gccagtgttt cgaggtcgac cgcctgtagc gttacagcca
    49321 gtgccggcgc ggccagcgca agccggttca gccgctcggt gagcagcggc atcatgcgtg
    49381 ccggcgacca gctcgggccg gccagccgga catcaagcgt ggtcggcgcg cacgcatgcc
    49441 tgcctcgctc atgctcgatg accagcgcga tacccgtggc ggcccggtgc tgcgctacca
    49501 gccatccgca gagttgctcg acgagccggc tcgccacgca gagcacgccg gccacgtgtt
    49561 cgacgcgctc ttccagttcg atgcgtccgg caaagacggc gggagccgcc atccaggcat
    49621 gcagttcggg ggcctcgccg tatgcctgat cgagggcctg cagcagcgcg gcgtgcgtgc
    49681 ggcgctgcaa ggcgccgcgc ggcaggcgcc gcagggcggc gagcgtgtcg cagccgatgc
    49741 cggccagcca gtcgaggtgg cgggcggcag ccggcaggca ggcggcgggc aaggcgtcga
    49801 gtcgccgcgc cagggtcgtc agcttcagga cacgccggcg ttggcgcgcc gatgccgcca
    49861 gcagccacgc gccgctggcg gtgggcgcca cgcccaggcg cgcggccacg cccaggccct
    49921 gcgccgtagc ggctatgcgg tggcagaggg cgcggatccc ccggaacaga ttcaggctgg
    49981 cggtcacgtc cagcagaatg gtgtcggcct cggtcagcgc cacttccggc gtgtattgca
    50041 ggagcgccag ggcggcatct tcgaccaacc gggcgtgcgc ttgggcatcg taggcgcgca
    50101 aggttgcgct cgggcacagt gcgttcaccc cgctggcccg cagtcctggt cgcacgccgg
    50161 cctgcgccgc caggggcgag caggcgcgca cctggcccgc ttcgaggacg accgccgcct
    50221 cagttcgcca gcgtgggcac tgactgtcca ggctcgcgtg cggcaggtgc acggctatcc
    50281 agactggcat catcgacctc ggcaatacgg cgcgctggat acagcggtac gagaatcggc
    50341 gcatcgcaga cgctgccccg gcgtttcagg atgcgcacgg atacgccgcc cgggacgggc
    50401 gccagggcca gacgcagcgg cgcggccgag gcttgcgcgg ccgccgccaa cgggcggacg
    50461 accacgaaca aggcgtcgcc ggattgggcg gccagttgca agcggcgcac cgccgcggcg
    50521 cgcatcgcgt tctgccagag caggacggcc gcaaaggcgc tgctgcgcac ggccagctcg
    50581 gccgcccaca aggcatcgac atcacgctcg ggccgcagcc acacgagacg tgccgggtcc
    50641 gcgccccagc cggcccaggc agcggcctgc ggcgcgtgcg gtggacgcac taggacaatg
    50701 gggcgcgcgt cgggcccgag gagcgcgctg cgcagcaggt gcagttcccc gacacccgcc
    50761 tgctggacca gcaattcggt caggttgccg cggggccagc ctccgccggg caactgcgcc
    50821 gatagcgcgg cgatgccagt gggcgcgtag cggcctggcg cccgggacaa ttgcgaggcg
    50881 cgccagaggg cagggtggat atgctcggga gccagtgagg cggaagtcat ggaggaaagc
    50941 aagagcagct gtttttactg gataaatata cagtattcat gccatcggct tgtaacagcc
    51001 tttgcttttg ctgcgcttcg ctgtcgcgat gtctgctggg cactaggcga cctggaccgg
    51061 ttgaccctgg tgccgcgacg gcctcagccc ggaacgcccg ggttggggcc tcggcctgac
    51121 agtcagccat gcggtagcgg ttgtcgcgac gcttcgcgca aagcacgcgt ccaaccgcct
    51181 gacaatacga atcgaggaaa ggtcagccat tgctcggcga ggatgcggac taaggcttca
    51241 gccggtccgg agaaccgctc gggttgtccg ggttcgccac gatggcagcg gccttgccat
    51301 gccagtgacg cagccatcgc agccagacag agcatcgccg ctatccacat gccaagcagc
    51361 agggcggcga ccatcgccac attgccagca ataaacgccg gtacagccgc aaggtgtacg
    51421 agcagggtgc tacgcaaacg atgggttcgc gcgtaatcct gccactgccg ttcgacgagt
    51481 tctacgagag gcatttgcgt atatcctttg cagtgcgaga tagggatgga aatccggaac
    51541 agcctaagcg aggaaatgtg ccctgtcttg aacgttctgg ctgtcaagaa ttcaatcatg
    51601 ctcattacac ccagttccgg cacgttcccg cggccttccg cgatggtcgt atggggagcg
    51661 ggagacgcgt caagcgcgca tcggcaccac agcgttcaac ttatcctcgc gctggctggg
    51721 aaccttcgca ttcgcggtgg cgagggtgag ccctggcact gctgcgacgg cgcgctggtg
    51781 cgcgcggacg cgctgcacga agtggacgca cgcggcgcca cggtactgat cggtttcatc
    51841 gagccggaaa gccggctggg cgcagccttg gcggaacgta ttgctgcgga tatcacccat
    51901 ctcccggcgg acgaggcagc acgctggcgc agcacgatct tcgcggccgg ttcgccgccg
    51961 ggcgatgaga aaatcaagag gtggctcgag gcagacctgc ttgacggtcg tccccaacct
    52021 accctacacc caggcgtgag tcgcgtcctt cgccacgtcc gccaccacct gcaggagccc
    52081 gacgcgctat cgctaagcgc tctagccgtc gttgccggcc tttcgccatc gcggctgatg
    52141 cacgccttta ccgcctcgat ggggattgcg ctgcgcccct acctgctctg gtcgcgcttg
    52201 caactagcct gcggagaact catgcgcggc gccagcacga ccgaggcggc gttgcaagcc
    52261 ggattcgccg atgcggccca tttgagtcgt acctgccggc gcatgctcgg aaccacgcca
    52321 acggcgatcg ccgccagcaa ggcgcgcagc aatattgatt aacccgcccg ccggttggcg
    52381 atcggacgcg ctgtgccaaa acctgcatcc aaacgcaaaa caaggcccta tatcgttaaa
    52441 tatagggcct tgtttttaat gggaatcttg ggcggaggag gatcgaactc ccgaccttcg
    52501 cattgcgaac gcgacgctct cccttcagtc ttatggccgt agacccgcat gaataaaggc
    52561 tttgcgggca ttagccgggt agggacgcta acgcaaggtg ccaaccagaa cttcaaaccc
    52621 ctggccagtg ggttggagct aaacggcgtg cgggcagtgc ttgccatggt gagcgcgggg
    52681 ctcgggttat cggtgatcca gttgtccgaa cccaacatct cccagctttt tcccgtgggt
    52741 gtggtggcgc tgcccgaccc gcccatgatc caattctcgc tgctgtttcg cgccagcgac
    52801 gaacacagtc gcgcgctgat cgctatccgg gtagcgggac ggggatggga agaaaactat
    52861 gcggccttgc ggttgtcttc ggcaacggcg tcttgctttc ctcggacaaa gactatccag
    52921 gcattcgacg cgctaaatat caccttatat caaaattaga tggggatgtg agatttccga
    52981 attggcgtcc taatttacga cagccctaga atgggttaag cgaaaaatga aaacagcaag
    53041 atgttgacat tggcgatgat tctgctgcgg ctagttctct atgcgaatca tgatctcaag
    53101 gggaaatcag atgaaaagca gtagaagaga ctttctcacg acgttagctg cgactgcgac
    53161 agtcggcacg atgcttccgg gatattcgtt ggcggccgag actccgaaaa ggggtgggca
    53221 cgtgcgtatg gcaatccaga atggatcagt ggcagacacg ctagaccctg cgaaaggcat
    53281 acattcaggt gatttcacta agcagttctg tctattcaat gcgctgaccg aattcaacaa
    53341 ttcgttgacg gcggagttgg cgcttgctga acatctggaa agtaccgatg gttcaaactg
    53401 gcatatcttg ctgaagcaag gagtgcactt tcacaatggt gatgagctga cggccgaaga
    53461 cgtggtcttt tctttggcta ggcataaaga tccggctacg gcatccaagg ttttttcaat
    53521 tgcgagcaat ttcaaggaaa ttaaagccgt aggcaaatac gaggtaatgc ttgcattgga
    53581 gcagccaaac tttgatttgc ctttgatctt ggggagtagc ttcttcctta tcatcaaaaa
    53641 cgggaccaaa gattttacaa agccagtggg cacaggccca tacttggtcg acacctttgt
    53701 gccgggcggg gggttttcgg caaagcgcaa ccccaactat tggaaaacag ggctgccgta
    53761 tctggattcc gtagagataa tcggtgtgcc tgataacgct gcccgagtga atgcggtact
    53821 ggccggcgat gtcgatatct gctcgttggt ggatcatcga tatgcggatc aggtcaaagg
    53881 caataataat gtccagttga ttgtcaacaa gctcggaatc tatacggatt tgattctgcg
    53941 gcaggatcac ccgataaccg gaaatccaga tttcgtttcc gcggtacgct acctgcagga
    54001 tcgcaaccgg ctcgttaaca ctgccatgcg gacatatggc cttgttggga acgaccatcc
    54061 tgtcgctcca tgggatccat attttttgga gggtctgccg cagagatcat tcgacttgga
    54121 taaagcaaaa tacttcatca agaaaagtgg cctcgcaggt caatcgatgg aggtcatttg
    54181 ccagcctggg ctttctcctt ccgtcgaagg ggcccttttt ttgcagagtt tgggtgagca
    54241 agtgggtttc aatttcatag tcaagcaagt cccggttgaa ggttattggg cgaattattg
    54301 gacaaaatat cccatcacct atggttcggt gacaaatcgt cctactgtgg gaatgatctt
    54361 cgaactttat tatggatcga agtcgccttt gaacgaggcc aagtggcgtg accccaagct
    54421 tgaccaattg ttgacttctg cttatgcgga aggagatttc aagaagcgca aagccatcta
    54481 tggtgatctc caaactttgg ttcatgagag tgcggggact cttgttccag tattcgatgt
    54541 gatactggat ggggtttcga cgacggtgcg tggttactac actaggccgt cgggtatgaa
    54601 tatggggttc cgttttgccg aggtggtatg gcgtgtttaa tacacaatat tcgatcgaac
    54661 ccgcagtggc ttccagttga gtgacatgat ttctatttct gcagatgtgg gtggtacgtt
    54721 caccgacctt gtgcttgtcg atactgaatc gaatttgcgc ctggttgaaa aagtcccttc
    54781 gcaaaaggga acggcggcag ccgtgctgga aggcatcggt ctgcttttgg ctagatcggg
    54841 caaaaaggca agcgatatca cgaggttcat ccacggctcg acaatcgcta ccaacgcctg
    54901 gttgacgcgc aacggcacgc ctagcgcgtt gctagtctcc cgcggtttca gggatacgct
    54961 ggaaatcaaa gatcagcggc gcaagcatct gtacaagctt gatgatcgcc gcgcgtcgcc
    55021 cctgatacca agatcccggg taatcgaagt ggcggagcgg atcgatgcac tgggccatgt
    55081 cgtgcttgag ttgacccaag ccgaggtcga aagaataggc aagatcgtcg ctgccttggg
    55141 tgtcgaaagc gttgccatct cccttttgtt cagtcacctg aatcccaggc acgagcggct
    55201 gcttgccaag tcgcttgttg atcgatgcca aggtctacta gtgtattgct cgtccgagat
    55261 caatccccag cttggcgaat atcctcgcgc gaatacgacg gcagcggcga gctatgtcgg
    55321 accggaactg caacgctatt tgctttctct cgaacagggg ctgcaggaaa tgggtttttc
    55381 atttccgctt cttctgatgc gcagcgatgg cggagtcgcc acgctgcgtg ctgtgttggc
    55441 gaatcctgcg accatgttgc tgtccgggcc tgccggtggt gtggtcgcat cgctggacct
    55501 agctcgtcgc gcgggtgtgc gcaatgtgat cacatttgac atgggcggta cgtcggcgga
    55561 cttttccctg atttaccaac aatctatcgg gacatcgtca gagcgcgtgg ttgatgacca
    55621 gatcctgcgg gttcccatgc tcgatattga aaccatctcg gctggaggtg gatccattgc
    55681 ttccgtcgat catgccggtg cgctccacgt cggtccccag tcggccggtt cctatcccgg
    55741 gccggcatgt tatggcaggg gaggaaagtt gccggcattg acggatgcgc tcgctgccct
    55801 gggcatcctg caacccgcgg actttgaaca tgctcagacg accctttcgg ccaacgcagc
    55861 gcgggaagcg atcgaaaagg aagtcgcgat tcctcttggt ttgtccgcgg atgagtcggc
    55921 gtatggcatg attgccgttg cctgcgcgca tatgcgtcaa gcgattcgtg ggcttacagt
    55981 cgagcgtggc catgatctgc gagatttttc actggcggct tttggcggtg ccgggcccat
    56041 atttgcggca ttcatgctta tggatctcga agtacgagaa gtcctggttc caccgaatgc
    56101 gggtgtgttc tctgcatgcg gactattgat ggcggatgtg cgacaccagt tccaacagcc
    56161 gttcagtgca ggcctggatg atgatcacag gatggatcta cgcgagcaga tccggaaact
    56221 gcatgagaaa gccgagattg cattgaagga agacaatgtt cttccgcata aacggcaagt
    56281 ctttatgtcg gcagatctcc gctatgttgg ccagtttcat gtggttcgag tgccttttga
    56341 ggtgactgaa gacagcatct gggatgcttc cgccttgaaa gaacgctttc acgacctgca
    56401 cgaaaagacc ttcggacact gcaatcgcag tggttcgata gaaagcgtga accttcgcgt
    56461 agagggggtc ggaatgatcg atcaattgga ctcccctggt aaggaaaagg ccatggatca
    56521 cgctccggcc cctgttggag tgcgctcgat cgtcatggat cggatgggaa cgcgtacgaa
    56581 ctgtcctgta ttcgagcgtg accaactttt accggggcat gtcctggtcg gtcctgcagt
    56641 tgttcgccag cgggatacca ctatctatct attgaaaggg cagtgtgctt ctgtcgatgc
    56701 acagggcgtg atcagtatcc gcatgcaagg ctgaaaacaa tgaagagagt attcaactcg
    56761 attgaccgag atatttttca gggccagttg ttgggtgtgg cagaggaaat gtcgaaggcg
    56821 ctaaggcgta gcgcttactc cccgatcatc tgggacatgt acgattattc ctgtgcgatc
    56881 ctgaccgtag atggcgagat tctgtcgcag gcagaaacga tcccggccca attggggacg
    56941 atggcgacag cttttcatgg cgtaagcagt gagattcctc tcgatcagtg ggatgagggc
    57001 gacatcctca tctgcaatga cccctacaga ggctgcgctc acacctcgga tctcacgctg
    57061 ttcagtcccg ttttcaacgc tggcgaatta attgcgattg cctccactat tgcgcatcat
    57121 attgatgtcg ggggcagcgt tccagggact gtttcgctcg agcatgttga cctgctctcg
    57181 gaagggctcg tcataccgcc ggtcaagctc gtggtggctg gggaaatcag ccgtttcgtg
    57241 ctggacctat tcaaatccaa tattcgttat gccgaaccta gtctcggaga cctatacgcc
    57301 cagatttccg gatgccgcat tgccgagcgg cgtattacgg aactagcgcg cagatatggt
    57361 aatgaaggct ttgccgagct tgccaccgct gtcctcgatt acggggatcg ttatacgcga
    57421 gagatgatcc gtaaattgcc agatgccacg ttcgaggcga ctaccttgat cgaagatggc
    57481 atcgtttctt cagatcctgt gatactcaaa gcatcgctga ccgtatccgg agattctctt
    57541 gccatcgatt tcactgggac aagtgaccaa cgttcgtttg gcctgaactg tccatgggcc
    57601 tcgaccgttt cgatggtgat gtatgcggtt aaatgtgtga ccgcgtcgga gattccatcc
    57661 aacggcggct gtagccggtc cttgaaaata acggcacccg agggttctgt tctgaatccg
    57721 aagtggcccg gtgccgttag cgctcgccac ttgtcgcagc aggccgtggc agacgtcgtt
    57781 ttgaaagcat ttgcgctggc gacgcctgaa ctgggctccg ccgctagcca gatttcctat
    57841 ccgactttcc gggtcgaggg ggtggacgat agagtcgggc ataaacggcg caagtatttc
    57901 atagcagata ttctcggtgg cggtgcggga gcgagcgcga aagtagatgg catggatgcg
    57961 attgacacgc atggcggcaa ttgcgccgtg ctatcggccg aagtgatgga aaccctggct
    58021 ccagttcgtg tcctgcgcag cacgctcgtt caggattccg gcggtatggg caagttccgt
    58081 ggcggtctgg cgatggagcg ggattatgag attctttcgt cttctgcaat cggagccgtc
    58141 aagctgcagc aactggatgt gaataccgca ccatggggat ttctgggtgg gggagatggg
    58201 gcaactggct tctcagtgct acatccggac actggtcttg cgaaaaacat cccgtttcgc
    58261 ggggaacgca taacgtatca caggggggat gtcatacgcg tgcgaagtgc gggagggggg
    58321 ggctacggga atccagagca acgccatcaa tccgatcatg agcacgatct atccgagggg
    58381 tatgtgtcca cccattccgg ctcacaatga cgaaagggtg gtttgcctga gagcaagatt
    58441 aatttttccg ggcttgcctt atggcgccat gcaagatagc tagtgcttcc tgtgccagtt
    58501 ccagcggcgt gttcagcggt gcgagcaggc gtacggtgtt gcgatgcacg ccgcacttga
    58561 tcaccagcaa cccgtctttg cggcactggt cgatcacagc ttgagcgaaa tcggcgtcgg
    58621 gctggaaggg gtcgccatcg cgcacgaatt ctaggcccag catggggcct tggccgcgca
    58681 cggtgccgat ttccttgaac tcggcggcca gcttgtccag ggcggcacgc agcgtctcgc
    58741 ccaagacgcg gccttgctcc aacacattct cttcttcaaa gacgtccagc acggccagcg
    58801 cggcggcgca ggccagcggg ttgcccgcat aggtgccgcc caggccgccc ggcgtgggcg
    58861 cgtccatgat ctcggcacgg cccaccacgc ccgacagcgg caggccgccg gccaggctct
    58921 tggccacggt gacgaggtcg ggctggatgc cggcgctttg gaagccgaac atctcgccgg
    58981 tgcggccgaa gccagcctgg atttcgtcgc tgatgagcaa gatgccgtgc ttcttggtca
    59041 gcgcgcgcag ggcctgcagg aactcctcgc cggccggcag gaagccgccg tcgccttgca
    59101 cgggttcgag gatgatggcg gccacgcgtt cgggcaggat ctgcgtggcg aacaggtcgt
    59161 cgagcgcgtg cagggcattg tcgctggaga cgccgcggta ggggtcgggg tagggcgtgt
    59221 ggtagacctc gccggcgaag gggccgaagt tctggcggta aggcgcgcta aagccggtca
    59281 gcgtcgagcc cagcagggtg cggccgtgga agcccccacg gaaagcgatg atgcccggca
    59341 cgttgcggta ggcgcgggcg atcttgatgg cgttctcgac agcttcggcg ccggtggtga
    59401 cgagcagagt cttgtagggt tcgccgccgc tgaccagctt attgagcttg gcggccaggt
    59461 cgacgtagac gtcatagccc gccacctgaa aggcggcgtg cgaaacgcgt tcgagttgct
    59521 tgtgcacggc ctgcacgacc ttggggtggt tgtggccgat attctgcacg ccgatgccgc
    59581 ccacgaagtc gatgtagcgg cggccgtcga cgtcccagag cagcgagcct tgtgccttct
    59641 cggcgaagat gggatgcgct gtgacgacgc cttgcggcac gttctggacg cgggatgcga
    59701 tgtgggcttg gttgctgcgg gtcatagcgg tgatacctcg tcggtgatga tgcgcggcgc
    59761 accggccgcg gcatggtggt ctgtgggaga gtgttcgttg gatgcccgcg cgtgggcgac
    59821 ttgttcggcc agtacttcgg ccaaatgcag ggcgcgtcgg ccgctgagct tgccgatctg
    59881 gttgcggcag ctgaatccgt cggcgatcag cggggcctcg ccgggcacgg ccgccagctt
    59941 gggcagcaat tccagttcac cgacgcggcg cgccacctcg gcggtcttgg cgtgatagcc
    60001 gaaggcgccc gccatgccgc agcagccggt gtcgagcacc ttggcgccga gttgcttctc
    60061 cgccgccgcg ccgccgcagg cgcgatggtg gcagtgggta tgcacgtggg cctgtgttgc
    60121 gccgagcagg cgcagaccgg cttcgcgggc gaactcggcc attgtgcggg tgagtttggt
    60181 cagttgcgcg gcacgtgggt cgtcggtcag cagcgccggc atctcgtcgc ggaacacgga
    60241 caggcagctg ggttccagca cgagcacggg cacgccagcg gcgatggggg cggccagttc
    60301 gtccagaatc tggagcaggt tgccgcgcgc ctgatcgagc aggcccgagt cgtagaaggg
    60361 ccggccgcag cagacgtgct tgctggtcaa gcggggccgg tagccggcgg actgcaccac
    60421 ggaaatggcg gcttccagca cgtccggcgt gaagccgttg ttgaaggtgt cggtccagac
    60481 gagaatgtcg cgctcgccgt agacttcgcc gctggcccgc tgcaggcgct tcgccgtggg
    60541 cgtgcggcgg aaggccttgc cggcgatctc gggaaaagcc gtgtccggcg ctaggccaaa
    60601 gaccttgccc acgcggcgca gcacggggct gcgcatggcg ccgttgacca gaccgctgac
    60661 gcgcgtggcg gcgggcagcc attggcccag ccggccgatc agggcgtcca tgggcgcgcg
    60721 caagcggttc tggtagtagc ggtacaggaa ctcgctgcgg tacttgggga tgtccacgtg
    60781 cgtggggcag tcgttcttgc agcctttgca cgacaggcac aggtccatgg agtccttgac
    60841 ggcgcgggcg tctttgtcct cagaaattac gccgccgttg agcagttcga agaacaggcg
    60901 cgccctgccg cgcgtggaat agctttcctc gcgcgtggcc ttgaagctcg ggcacatggt
    60961 gccgccatcg agcgaccggc acttgcccat gccgatgcag cgctcggttt cgcggccgta
    61021 accagtgccg gggccgtcgc ccatggggta ggtgaacagc ccgcgggcgg tcgagcgatg
    61081 gtaatccggg cccatgcgca gattggcgtc cacgggcatg gcgtcgatga gcttgccggg
    61141 gttcatcagt ccggcggggt cccacagggc cttgaattcg cggaaggcct gcatcaggcg
    61201 cggcgtgaac atcaggtgca ggaactcgcc cttggcctgg ccgtcgccgt gctcgcccga
    61261 aagcgagccc tggtatttga cgaccagggc ggcggcttcg gcaagaaagg cgcgccagtc
    61321 ctgcacaccg cgcttggatt gaaggtcgaa ggtgatgcgg gagtgaatgc agccgtcgcc
    61381 gaaatggccg tacatattgg tcttgtagcc ataacgtttg accagcgccg agaactcgcg
    61441 caagtacttg cccagcaggg caggttccac ggctgcgtct tcccagccga ccgtcgggtc
    61501 gggccggccg gccacgcgcg acagggaggt ggccgaggcg ccggtttcac ggagggtcca
    61561 tagccggttc atcagcgcgg cttcatccac taggcgtgcg ctgggccggc tgggcaggct
    61621 ttgcgccacg gagacggcgg cgcgcgcctg ggtcagtgcc tcgttgcgcg aggctgcgcc
    61681 gaattccgcc agcagccagg cattgccctc gggcaattcg gcgatatcgt cgagcttgag
    61741 gcccaattcc ctgagtccgc cgataatgcc gtggtccagg ccttccatgg cgatagcgct
    61801 caagggcagc aggtccggca cgctgtcggc ggcatcgaag atggtgtcga aacccagtac
    61861 taccagcaca cgctcgggcc ggccctccac cagggtagtt tcggcgcgca gcacggtggc
    61921 gcaggtgcct tccgagccta ccagcgcgcg ggcgatgttg aaaccgttct cgggcagcaa
    61981 ctggtccagg ttgtagccgg acacgcggcg cttgagcttg ggaaagccct ggcggatgtc
    62041 gttggcatag cgctcggcca gttccttcag gccgcgcacg atgcgtgcct gtccgccgcc
    62101 cgcggtcaag tgggcggcgt attcggcgtc atcggtaggg ccgacccaaa agcgttcgcc
    62161 cgagagggtc aacacttcca ggcgctcgat gttctcgacc gtcttgccgg ccatgaccga
    62221 gtgcgcgccg caggagttgt tgccgatcat gccgcccagc gtgcagcggc tgtgcgtggc
    62281 ggggtcgggg ccgaaggtca gtccgtggcc ggcggcggcc ctcttgagct ggtcgcagat
    62341 gacgccgggc tgcacccggg cgacgcgtcg ctgcgggtcg atctgctcga tggcgtgtag
    62401 gtgcttggag tggtcgatgc agacagcgtc gttgactgct tgcccgcaca tcgaggtgcc
    62461 cgcgccgcgc ggcagcacgg cgatgcccaa ctccccacag gcgcgcaccg cagtctccag
    62521 gtcttccatg ctgcgcggga tcaccacgcc cagcggcggc tgacggtagt tggaggcctc
    62581 cgacgcgtag agcgcgcggc cggcggcgtc gaagcgcagt tcggccgtgg tctgggactg
    62641 caggcgccgg cgcagctgca gcagccggtt gaggtcggtc ttggcgccgc ccggggtcaa
    62701 tgccgtggac atctgttcct cagtcgagtt ggccttggca caggtacttg atgtgcatgt
    62761 attcctgcag cccgtgctgc gagccttcgc ggccgtagcc ggagtccttc acgccgccga
    62821 acggcgcggc ttccgcggcc agggcgcctt cgttgatgcc cacgatgcca gtttcaagct
    62881 cgtcggccat gcgccagatg cggcgtacgt cctgcgagta gaagtacgcg gccaggccga
    62941 agggcgtagc gttggaagct gcgatcacgt cggcttcggt ggagaagcgc gtgatgggcg
    63001 cgacggggcc gaaggtctcc tcgcaggagc agtccatggt cgcttccaca tgggtcatca
    63061 cggtgggagc gtagtaatag gcgccggcct tgccttcggg cgagatgcgc ttgccgccca
    63121 ccacgatctc ggcgccgcga ctgaccgcgt cgttgacgtg gcgctcgatt ttgtcgacgg
    63181 cgcgggcatt gatcattgga ccgatctgcg aggccgggtc ggtggccggg ccgaccttca
    63241 gggcggcaac gcgcgcggcc agcttctcga cgaaggcgtc atgcacggcg tcctggacat
    63301 agatgcggtt tgggcacacg caggtctgcc cgccgttgcg gaacttggcg gccatcatgc
    63361 cttccaccgc ggcgtcgagg tcggcgtcct cgaagacgat gaagggcgca ttgccaccca
    63421 gctccagcga cagcttcttg agcgtgtcgg cggagcggcg tgccaggtgc ttgccgaccg
    63481 gcgtggagcc ggtaaaggtg atcttgcgca cccgggcgtc gtccagccac acgtcgacca
    63541 cttcgccagc ctgctcgcgt gaggccgtga cgatattgag cacaccgggt ggcaagccgg
    63601 cttcttcggc cagcttgacc agcgccagcg aggtcagcgg cgtgtcttcg gctggcttgc
    63661 agaccaccgt gcagccggcg gccagggccg gggcgatctt gcgggcgatc atcgcggctg
    63721 ggaagttcca gggtgtgatg gcggccacca cgcccaccgg ctcgcgcagg gcgaacatac
    63781 ggcggccggc cacaggcgcg gggatgattt cgccgtcggc gcgcgtggct tcctcggcga
    63841 accattccac atagctggcg gcatagagca cctctccctt gccttcggct aggggtttgc
    63901 cctgttcgct agagatcagg cggcccaggt cctcgatttg cgcgacgatg aggtcgttcc
    63961 agcgcttgat gatctgcgcg cgctgcttgg cgggcacctt gcgccaggtt ttgaaggccg
    64021 cgtgtgcggc gtccagcgcc gcgcgtgcgt cggccgcgcc gctatcgggt acgctggcga
    64081 agacttcacc ggtggccggg tcggtgacgg gcagggtgcg gccgctccgg gcggcgtgcc
    64141 atgcaccgcc gatgcagttg aggccgggga gaagatcgga attctggaaa gtaaagggca
    64201 tggaatgttt gtcctgggga gcgcggcaaa tgagagaaat tggatcacgc aaggctcggg
    64261 attttgttgc gaattgcctc tatccgagcg tgggaactgc tgtttttgag agtcatttca
    64321 gtatggcttg ccgaatgtgc atgctggtct agattaaaat cttcgcttac ccgcaggcca
    64381 ccgatcatgt ttcccacgcc taagctcgac cgccttgact tgcgcatcct cgtccagttg
    64441 cagaagaacg ggcgcatgac caacgttgac ttggccgagg ccgtgggttt gtctgctagc
    64501 ccctgcctca cgcgcgttaa acggctagag gaagccggct acatcggtgg ctacagtgcc
    64561 aagatctgca tggacaagct gggcgacgtt ctgacggtgt ttaccgaagt gaccctgtcc
    64621 gaccatcgcc gggaagactt cgcccgtttc gagcaggctg tgcgggatat cgacgagatc
    64681 gtcgaatgcc acatggtcag cggcggctac gactatctgt tgaagttcat tacccgtggc
    64741 gtgaatcaat accaggagct gatggaaagc ctgctcgagc gcaatatcgg catcgaaaag
    64801 tacttcagct ttattgtcat caagtcgcca ttcatcaaga cacactatcc gatcgagcga
    64861 ctgttcccga ccgggttatc ctgaaggaat ccttgctcag gcaaatacca cgaagacttt
    64921 gcgcagcgtt tggaatcgcg ccttgctgga gcgtcaggaa gttgtgccga cgttttctgc
    64981 ttcggtgaag gcggtttcca tcaggccggt tgctgtgtgg cgctccagcg acatgacgag
    65041 gtcgataatc ttttctgctt gattgcccgg catgagaggc cagagtgcgt cacggaactt
    65101 gccttgcgtt tgcgccatgt ccgcgattcc gcggcgctct gagcccagcg gttcggaaat
    65161 gaggatctct cggctggatt ctccggcaag tttgacgacg actttggtgg gaacggacat
    65221 gtcgatgcgc cgcagggcgt cgcattctgg gtcatgatgg atgtggatct tcgagaaaag
    65281 cttttggagc tcttcagaac cgaaatccgg ggctcgcagc gcggcgttta cgtcgttgtg
    65341 caatagcgct actgcaatca ggaagcgcac attgaattgc gcgtcgacca ggttggcggg
    65401 atagggcgag tccagtgtgc cgcaaaactc cttgaaggca tagaggtcga tggactgcag
    65461 cttgcgcaag tcgatggatg gcttcatctg gagtatcgcc tccagcgcgg gatgcgacca
    65521 ccggcagcat gcccagggct tgaattcgac atggcggatg agccagtcgt ctcccggcag
    65581 ggcatccagg cactcgggcc ggaagcgatc ggatccatag atcttccaga atccgaactc
    65641 ttcagagaaa aagtgctggt ctccaatata gccttgcttg gccagaaaag ccgacatgac
    65701 accagccgag gcggcggctc cataagtatt cttcaaccaa gtgtagccac cttgcggatt
    65761 ggtccgaaca atcataggaa ccggtgcctg ctgggccgca agaccgaaac cttgtatcca
    65821 ttgatcatga tccagtttca gcaacgtgcc tgctgcgacg taggcgccga agatttgcca
    65881 ggtggcatag ccgatatctt tggaaggcgc agcatcgctg gggcacagag atgcaccgat
    65941 ccgcaacgat gcctcgtagg ccttgacgat ggcaagcagc atgtcttggc cacttcgctt
    66001 tagtacgtgc gcgcaggcta gtgccggacc tatgatcgtc gcccctggat gtgaaggcgc
    66061 gtatcggtag caatcgtcga aatcgaggat gttggccgct tgtgcgtaga gatttgccgc
    66121 gtcgaaaatc cctgcttcga gggcccctcc gatagggact aggccggttt tgttggcaga
    66181 gttgaattcc aggacgtgac ggtaatcggc gatgggctga gcatgaagac cggccagcaa
    66241 gcaagcaagc ccgtcgagca agcattgctt gcccgacgtt agcacgtcgg gcgggacgtc
    66301 ttcataccgg cacccgttta gccactcgac catcggagag aatccctctt gagcggcgct
    66361 atacatgggg ctatgagtca gagtgggcat gttttgcttc aaaaacgggg gattggatga
    66421 ggtcgagccg cgccttggtc caagcctatg ctgcggttag cacggaaggc cgtatcggta
    66481 tgtcccgtag aagaacgaga tgggcagaca gtccagtcaa atcggtttga tgcgtacctg
    66541 ggtgtccgcg cgtataacgg gcatgttttg ccgtcggttg attccctccg gcatagatat
    66601 tttcgacagc ctaagagccg gtatgagtga ggtcaaatac gatttgacgc cgacttcatg
    66661 cgtttttgat atgggttggg ttaacccacg ctttcccgca gggctttggc aaaagccttg
    66721 gccagcatat tcatagggct gttgagcggg taaacgagat aggtgggaaa ttcgatgcgg
    66781 ggagaaaacg gcagtatggc aatgcgcccc gcatagttgc gtgccaccag cggattggac
    66841 acgctcacac ccatgccgca ggacaccatg tggcagatgg ctgccgcgaa gtgtgactca
    66901 taagcaagta tgcgtttttc ttccccatcg atcaggcacg cctcatcgat ctgcttgcgc
    66961 atgacgtcgc tggatttcag cgacaggaat ttttcttctg ccaggtcgcg cggcgttacg
    67021 gcacgtttgc gcgtagccag ccggtgctcg agaggcgtga tgcacacgcc gacgttgcgc
    67081 tgcaagactt cgtgttcgat gctggtgctg ttgaaaattt cggacgcgat gcctacgtcg
    67141 acatagccag tggctagcca ttggcaaacg gttaacgaat cggtgacctg caaggtgatg
    67201 tcgacgtcag ggtaaacccg cgcaaacaag ctaatggctt gcggcgcgac tacgatggcc
    67261 atcgacggca ccgacgcaat acgcagccgg cccgagccac gcttgcgtat ggtgtcagcg
    67321 gcctcttcca ggcctcgcaa gccggcgaac gtatgttcca catcccggaa gaagacctgg
    67381 ccttcgagcg tcggcaccag cttgccagcg tgccgctcga acagcttcag gctgatgcgc
    67441 cgttccaatt ggccaatgac ccggctgatg ttgggttggg aagtgtgcag gtccttagcc
    67501 gcttgtgtca tggaccccga cagcatgacg gcacgaaagg cctctatctg gcgaaaattc
    67561 agcatcagga cttgctcata tcaaaaatgt ataccctgag attatatgtg aatttgtcat
    67621 atggctggca gattcttaaa ctctcttgga acgattggat tattttcata aaaacggtca
    67681 aggggatgcc gccaggcatc ggaaccgggc tccgaggaaa tggacatgct ggattttgct
    67741 ccggacgaaa ttaagctgcc aatggggcat ttcattggtg gcaaactggt tggcggcggc
    67801 cccatgctgg acgtactgcg cccctccgac ggcaagcgga tgggcggcat tccggatgcg
    67861 acgccagacg tggttgatca agccgtgcgc aacgcgcacg aggcctggcg cgccagtggt
    67921 tggggtgcgt gcccaccgcg agagcgagcc cgtgtcctgc ttcgatgggc cgatctgctt
    67981 gacgctcatg tggacgagct tgcccgcctg gaggcggtgg gatccacgcg tccgatccgg
    68041 gaagcccatg ccttcgatgt gccttttacc tcggaatgca tccggttctt cgccgagtgt
    68101 gcagacaaat tcggcggcga cgccgtgccg accaagcgtg acagcctggg tttaatcctg
    68161 cctgaacctt acggcgtggt gggtgctatc gcaccctgga atttcccgct gtcgatggct
    68221 tcttggaagt gtggtccggc tctagctgcc ggcaacgcga tcgtgctcaa gccgtctgag
    68281 ttgacgcctt tttcaatcct gcgctttgcg caattggccg tcgatgcggg cgtcccgcct
    68341 ggggtgttca atgtcgtcca agggcgaggc gccgaggcag gtgcttcctt agtgcgtcat
    68401 ccgctcatcg gtaagatatc ttttacaggc tcgacgctga cgggcgccgc catcatgagc
    68461 gagtcggccc gtcacggcac caagcccgtc acgctggagc taggcggcaa gagcccgcaa
    68521 ctcgtctatg acgatgcggg cgacaccgct gcggtagctg gccgaatcgt ccggggattt
    68581 acctccaatg ccggccaagc ttgcgtttcc ggcacgcgcc tgatcgtgca acgtggcgtt
    68641 gccgagcgcc tgatcgaggc gatcgtggcg caagtatcgg gtttccagcc aggcctgact
    68701 tggcgagaag ccacacgctt tcctcctttg atcaacgaac gacaggcaca gaaggtccac
    68761 aacgccgtgc gggatagcgt ggccgaaggc gccacgacgc tttgcggcgg ggatttcttt
    68821 ccaggggatc tgggcggtta tttctatcgg ccaacgctac tggccggcgt cgatagcact
    68881 atgcgggcgg tgcgtgagga aatcttcggc ccagtgctga ccgtgcaagt cttcgacgat
    68941 gaagaagagg gcttggcgat ggccgatcat ccgacctacg gcttggccgc cggggttcat
    69001 acccaggaca ttggccgggc cttgcgcgct acacgccgga taccggcagg gacggtgtgg
    69061 gttaatcgct tcggccgcac ttcggacttc atccttccga ccgggggctt caaggggtct
    69121 ggcctgggca aggacctcgg ccgccaggcc ttcgaggcca acctccgcta caaaagcgtg
    69181 ctggtcgatt tctgactttt ccgattccgg gcggcaaagc ccacttttcc aggcgattgt
    69241 ttctaaggta gcaccatggc gattttcaaa cccaaataca tcactttcga ttgctacggc
    69301 acactgatca atttcgatat ggccgcagcg gcccgcaccc aatacaaggg caaggtgagc
    69361 gaggcgaaca tgtccgggtt catcgagagt ttccggggct atcgcctgga cgaggtgctc
    69421 ggcgcctgga agccgttcca ggcggtgata tgcaacgcag tggagcgcac ctgcaggaaa
    69481 tggggagtga agtacgagga ggccgatggc ttggcgatct acaatgccgt gccgggatgg
    69541 ggaccgcacc cggacgtgcc aacggggctg tcggttgtgg ccaaggagtt tccgctggtg
    69601 attttttcga acgcggccga tgagcagatc agccataacg tggcaaagct gggggcccct
    69661 ttccacaagg tcttcacggc tgagcaggcg caggcctaca agccgcgcta tcgggccttc
    69721 gagttcatga tggattcact gggttgcggc ccgcaggaca tcctgcatgt gtcttccagt
    69781 ttccgctatg acctgatgtc ggcgtacgac ctggggatca agcacaaggc gtgggtgaac
    69841 cggggccatg agccggcgag tcccttctat gagtacaccg agatcaagga caccagcggt
    69901 ctggccgcag tggttggtct atgaaactcg aaccgttctg gctcgatacg gcaccggcct
    69961 tcacggcggg cgcaacggag gcgttgcctg cccgtgccga tgtcgtggtg attggcgccg
    70021 ggtttacagg cctgtcggct gcgcgcacgc tcgcgcgcgc aggcgtcgac gtggtggtgc
    70081 tggaatcggg gcggatccag ggcgaagcct cgggccgaaa cggcgggcat tgcagtcctg
    70141 gtacagcgca aggcctcgcg gacctgatcg acaaatacgg ccttgatgcc gctaaacgct
    70201 actaccagac ttatgacgat gcggtcgatt tcgtcgagcg caccattgtc gaagaaagta
    70261 tcgattgcga ctttgtgcgg catggcaagc tgaagctggc gagcaaggcc aagcacttcc
    70321 ctgggctggc gcgcaaccat gatgcgctgc gcaagcatct tggcctgtcg gtcgagctgc
    70381 tgtcggcgtc ggacgtacgc cgggaactgg actcggacgc gttttacgga gggctgctgg
    70441 atcctagggg tgcccagatg cacatgggca agttcggcac gggtttggcg atggctgccg
    70501 ccagccatgg cgcgcgcatc taccagcaca ccgcggttac gggactggcc agcctggggg
    70561 gagaccggca tcgtgtcgcg acgacgcagg gcgtgatcga ggctgggcgg gtgctactgg
    70621 ctacgggccg atccgaccat ggcccgttcg actggtggca gcggcgcatt gtgccggtgg
    70681 gcagcttcgt cattgtgact gaaccgatac ccgatctgct ggatcaggcg ctgcctcagc
    70741 gtcgcaatta tgtgacgagc ctgaattttg gcaattattt ccgcacgact gccgaccacc
    70801 ggctggtgtg gggtgggcga gcgcgttttg cacgcagcaa tccggcttcg gatctgaaga
    70861 gcgggaagat cctcaaggcc agcctcaagt ctctcttgcc tgtactgaag gacgttcgga
    70921 tcgattattg ttggggtggg atggtcgaag cgacggcgga ccggctgccc tcagcgggca
    70981 agcatgaggg gttgtactac gcggtagcct acagtggtca tggcacgcag atgtcggtgt
    71041 acatgggcga tctcatggcc gacattctga taggcaaggc ccgcgtaaac ccctggacac
    71101 gagagaactg gcccgccttg cccggctatg cgggccgggc ttggtttctg ccgctggctg
    71161 gcgtgtattt ccgggtcaaa gacattcttt attaatcgtt tcttggctgc gtgccatggg
    71221 ttttggggta atcccccagg agtatgtatg gctttgaaag acatgggctc gaatgaatag
    71281 cctcatcctg cgcatgatcg gccggcgtgt tggggtcggg ctgattacct tattcatcgt
    71341 gtgcgcactg gtgtttttca tgtcgaacat gttgcccggc gacgcagcgc aaatggcctt
    71401 ggggcaggcg gctacacctg agactgtggc ggccctgcgt cagcaattcg gcctggatca
    71461 accggcgcta ctacggttct tcatctggtt gggagacctg gtcacgggaa actttggcat
    71521 ttcttatgcc ggcggtgtgc cggtgaaaga tatcgtcatg gcacgcattc cgaattccct
    71581 aatgctggct gccgcggcta cggtcacatc ggtgcctatt gcactggtgc ttggcatcgt
    71641 ctcggccatg tacagaggaa ctcgcctgga ccgtgtgatt gggatcgcca ctatgtctgt
    71701 ggtcgcagtg ccggaattct tggtggcgac attggccgtc ttgctttttg ccgtaaaact
    71761 gcattggatg tccgccttat ccttggggac caacacttcg tcggtgactg ggtttttcaa
    71821 ggcctatagt cttccggtct tggtgttgtg ctgcgtggtc gtggcgcaga tggcgcgcat
    71881 gacgcgcgcg gcgctcatta accagttgga tgctgcttac gtcgagatga cggtgctcaa
    71941 gggactgaag ccagcccgga cggtgcttcg ccatgcgctg cccaacgcta tcgggcccat
    72001 cgttaacgcg atggcgctca gcctatccta tttgctgggt ggggtcatca tcgtggaaac
    72061 gatcttctcc taccctgggc ttgcgagtac gttggtcgat gcggtcagca accgggatct
    72121 gcccatcgtg cagttttgcg tgttgttctt cagtgcttgc tacctattgc tgctgttgat
    72181 cgccgacatt ttcacgatcg tcttcaatcc gaaactgcgc tgaccatgac cagaatccta
    72241 cgttttccgg gcgcatgcgg gcgtttcttt ctatctttgc caatagcagg gcgtgtcggc
    72301 ctatgcatat ccactttctg gatattcatc gcggtatttg gttcgtggat agctccatat
    72361 ggcgcaacgg actttactga tggccagatg ttcggtcctt gtaccctggc ctatccattg
    72421 ggtaccgact atttgggacg cgatctgctc agccgcgttc tgtatggcgc acgctattcc
    72481 atcggcctgg cactggtggc cacgctactg gcaagcacat gcggcacatt gctggcgttg
    72541 atctcgaccg tcattggcag gtggttcgac gaaggcttga gccggctggt cgatgccttg
    72601 ctgattcttc ccagcaagat cctggccttg ctgatggtgg ctatatatgg ttcttcgcta
    72661 ccggtgctgg tgctgaccgc ggcggtgacc tacatgccgg gcgcctaccg catggcccgt
    72721 tctcaagcgg tcggcctgaa cgcgatggag tatgtcatgg tgtcgcgcct gaacggtgaa
    72781 agcaaactct atatcgcctg ccgggatatc ctgcccaaca tgatccatcc catgatggcc
    72841 gattttggcc tgcgcttcgt ctacaacgtc ctgatgctca gtagcctgag ctttctcggt
    72901 ctgggcgtgc aaccgccgaa cgccgactgg gggtcgttgg tgcgcgagaa tctttcgggc
    72961 ctgtcgtcgg gggcccctgc gatcatcatg cccgccctcg ctatcgccac gctgacgatc
    73021 ggcgccaatt tgttcattga cagcctggcg gcgcgcaagc gcctgggcgc gcgggggtag
    73081 accatggccg ctcaaccatt ggtggaagtc tccggcctgc aggtcgtggc gcgcggcgag
    73141 gatggccgcg agacgcctat cgtgaaaaat gtggaatttt cgatcgccaa gggcgaggtc
    73201 cttgccctca tcggagagtc ggggtccggc aagaccacga ttgcgctggc gcttctgggc
    73261 tatgcccggc gtggctgccg catcgctggc ggccgcatcc gtgtcggcga ttcagagatc
    73321 gtttcggcct ccccgcgcga cctgtacgcg ctccggggct ctcgcgtggc ctatgtggcg
    73381 cagagcgcat cagcgtcgtt caacccgtcg ctcaggatca tggaccaggt gatcgaaggc
    73441 gtgtgcgtgc atggcatcat ggaccgcaag gcggcgcggg aaaaagcggt ggatctgttc
    73501 cgatctctgg cgctgccttc accccagact atcgggaacc gctatccaca tcaggtgtcc
    73561 ggcgggcaat tgcagcgcct gatggcagcc atggcgttga tgtccgatcc cgaggtggtg
    73621 gtgttcgacg aaccgaccac tgcgctggat gtcacgacgc agatcgaggt cctgaaggcc
    73681 ttcaagcagg ccatcagaga gcatcacatc agcgcgatct atgtctcgca cgacttggcc
    73741 gtggttgcgc aggtggccga caaagtcgtg gtgctgcgca acggcgagat ccaggagcag
    73801 ggcgtgctcg ggcaaatcat cgatgcgccg gcgcacccgt acacgcgcag cctgatgtcg
    73861 gccgccatac cggtttctcg cctggtggac aacgacgccg ccctcgcggc gcgacaaccg
    73921 cccttgctgg aaatcaaggg gatgaccgcg ggctatggca gcctggacgc atcgggcatg
    73981 cccacgatca ggattctcga cgacattgac ctggtcgtgc ggcgtggcac cacgatgggc
    74041 gtcatcggcg aatcgggttc cggcaaatcc acgctggctt acgtaatcgc cggcctgctg
    74101 gcaccggcga aaggccagat cacttttgcc gggcaacctc taccagggca tctggtcgac
    74161 cgagaccagg atctctatcg gcgcatccag ctcgttttcc agaatgccga caaggttttg
    74221 aacccttccc acagcgtggg cgaaatcctg gcgcgtcctt tgaaacgatt cgaacgctgc
    74281 gcgcccgggc agattactcc aagggtcgac cggctgttgg acatggtgaa actgccagcg
    74341 tccatcgcga agcggcgtcc ggcggagttg tccggcggac agaagcagcg catcaacctg
    74401 gcgcgcgcct tggcggccga gccggagctg attatttgcg atgaagtgac ctcggctctg
    74461 gacactgtgg tcggctcggc cattctggac ctgctcgtcg aactgcgcca tgcattgcaa
    74521 atgtcatata tcttcatcag ccacgacatt tctacggtac ggaccacctg cgacgagatc
    74581 acagtcctat accgcgggcg taaagtggat tcgtgcgagc gcgaggcact gctttctcag
    74641 cctcgccatc cctataccga gttgttgatt gattccgtgc cggaactccg gtcgggttgg
    74701 ctggaggagg ccagtgctcg cctgggttcg cgccaggacg agattgctgc agcaggttag
    74761 ctaataaaaa aacccgcgag gatcatgaat ttcgcggggt tttcggcagc gtggataggc
    74821 cttgttatgc tcgccaaact gattcggcga atcgatagcc catattcatg cctgataggt
    74881 tgggtttgta gcccctgatt tgcttcgaaa cgccgtccag aatggcggtg aaaacgggaa
    74941 tgatcgttcc tgagctctca tgcaccaggg tctgcatgtc gccgtaaatg gccttacgct
    75001 tcgtgaagtc accttcagcg cgagccgcat ccaggagttg atcgagcttg gggtctttcc
    75061 attgggcttc gttggtggtc gacgtcgagc ggtagaagag gtcgaaaatc atgcctacgt
    75121 tgggccggtt ggaaatggag ccgtaggtga tcggaaactt ggtccagtat gtcgaccagt
    75181 agccgtcggt aggaacttgc ttgatcttaa agtcgaagcc aaccttcgcg ccaaatccct
    75241 gaaggaattg cgcgccctcc accgaagctg gaatgccagg ctggcatatg atctcgatgg
    75301 attgaccggc taatccactc ttttgcatgt ggtatttggc cttgtccaga tcgaagggcc
    75361 gctgcggcag accttccatg aagtaggggt cccagggggc aacagggtgg tcgttggcga
    75421 tggcgccata gccttgcatg acgttatcga gcatgcgctt gcgatcttgc atgtatcgga
    75481 cggccatgac gaaatcctgg ttgcccgcaa tggggttgtc ctggcgcagg atcaaatctg
    75541 tataaagggc cagtttgttg acgacaagct cgacattgct gctgcctttg acctgtttgg
    75601 cgtagcggtt ttcgaccagg gaacaaatgt cgatgtctcc cgccagcacc gcgttgacac
    75661 gggccgcgtt gtctggaacg ccgaagattt caacggagtc caggtgcggc agacccggct
    75721 tccagtagtt cgggttgcgc ttggccgaga acttgctgcc agccacgaaa gtgtcgagta
    75781 tgtacggccc cgtgccgacc ggcttggtga agtccttggt gccgtttttg acgatcagga
    75841 aatagctgct gcccagcgcc gaaggaaggt caaagtcggg cttggcgatc acgagggaaa
    75901 cttcgtattt gcccgtggcc ttaatttccg tgaattcgct ggcaatggca aaagccttgg
    75961 aagcggtcgc tggatctttg tgacgggcta gcgagtaaac cacgtcctcg gcagtcagtt
    76021 ccgaaccatc gtgaaaatgt atgccttgtt tgagggcgat atgccaatgg atgccatcgc
    76081 tgctttcaag acgttcagct aacgctaggt cagggatcaa tgaatcattg aattccgtca
    76141 aagcattgaa caggcagaac tgcttggtcc agtccgcgga atgagcgccc ttggctgggt
    76201 ctagcgtgtc agatacagag ccgttctgaa cggcgaactt gacgtgccct ccttttttgg
    76261 gcgcttcgtc ggcccaggag aagctgggca ggctggccaa ggtcccagcc gtcgtacata
    76321 tggaaagaaa acctcttctg ctcgtttgca tagcaattcc cctcaaagcc atagttgttt
    76381 cgctgtagaa agcaagtcag cgttggtgaa tctgcttaag tctatgggct attgacaatg
    76441 cgctcaaata cgaattaccc aatgcccgat acatttttga tatggcagga acgcccggct
    76501 caggcacata tcaaaaaaac atagcctcct gccgcatacg aatttgtcat tctaggggga
    76561 ggttcttaaa ctcgctccaa actatcgaag caactatttt gagataaggt gggccagaag
    76621 gatgggtcta agaatcggag tcgatatcgg cggatcattt gccgatttcg cggtgctgga
    76681 cgaagctgat cgtacgatcc gtacgctaaa ggtattttct cggcccgacg aacctggcag
    76741 tgaagtgctt gctggcatgt cgcaattggc gcaacgctat ggcatagatc cgagggacgt
    76801 gacgtatttc acgcatggca ccacagtcgg tgtcaacgcg gtcgtgcagc gcaagggttt
    76861 gaagctgggt ctggtcacta cgcgcaattt cgaggacgta ctcgacattg cgcgcttgaa
    76921 aatccccgac atgtaccatc tgaagtccaa gcgtccggcg ccgctggtga cgcgcgaccg
    76981 ggtgttcggc gtggtcgagc gcatggcggc cgacggtacc gtggacaccc cggtggacga
    77041 ggccagtgtc ctggccgcgg ccgaagcgct gcgcgcggcc ggttgcgagg gtgtggtggt
    77101 gtcactgctg cacgcctatc gcaatcccgc gcacgagcag caggtcaagg cgctgatcca
    77161 gtcggctatt cccggcatgt tcgtttcttg ctcgcatgag gtttggccta tcatccgcga
    77221 atacgagcgc acactgaccg cggtgatcgg cggttatgta cagcctcgcg tctcgcacta
    77281 cctgaccagc ctgcaaggcg cgctcaagca agctggggta ggcgtggacc tgaaggtcac
    77341 caagtcgaac ggcggggtga tgagcgcgga gtatggcaag aacaattgtg tgcagatgat
    77401 cctgtcgggc actgcctccg gcgtgatcgg tgcggcctat gtggctcagc agtgcggcgt
    77461 caagcgttgc atgagcctgg atatcggcgg cactacggcc gacatggcgc tcatcatcga
    77521 tggccagccg caatacgcca ccggggagta tatcggcgac ttccagattc acatcccgtc
    77581 cgtgtcggtg tcgtcggtcg gcgacggcgg cggttccatc gcctgggtgg accgcttcgg
    77641 cgtgctcaaa gtcggcccgg agagtgccgg ctcgaacccc ggtccggtgt gctacggccg
    77701 gggcggcacc cagcccacta tcacggatgc tttcgccgtc atgggggtga tcggcagcgc
    77761 cgagcttggc tacaacgcag tgaaggtaga cgtccaggct gcgcgcgagg ccattgctcc
    77821 gctggcggcc aggcttggca aggacgtgct cgccacggcc gaggccattg tcgaagtgtc
    77881 ggtctccagc atgtatgcgg gcgttagccg catcatctcc cgctttggcg tggatccgcg
    77941 cgagttcacc ctcatgcctt tcggcggtgc cggccccatg atggcttgct atcttgcgcg
    78001 tgcgctgaat gtcggcaagc tgctggttcc caccacgccg ggcgtgctgt cggcgctggg
    78061 ggggctgatc gccgatctga agaacgactt cgtcaagact acctactacg acctcacgcc
    78121 cgcaaccatc cgggcgttgc aggacgaaat gggccggttg gaagcgcagg cgcgcgcttg
    78181 gcttgctgcc gagggcggcg atccggatca ggccgtaatt gatgtgtcgg ccgacatgcg
    78241 ctatcgaggc cagtcgtttg agatcgacac gccgctcaag ccccaatggc tgcgtgacgg
    78301 cgacatggcc tcgatcagcc agaccttcca cgtcgagcac aagcgcctct acgggcacag
    78361 cgacgactcc gcgcccctgc aggtggtggc cttgcgtctg gtcatcacct cgcggacacc
    78421 caag
//
LOCUS       DASWPO010000094         9300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1181606, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000094 DASWPO010000000
VERSION     DASWPO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181606"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VGC69_05775"
     CDS             <1..609
                     /locus_tag="VGC69_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0294828.1"
                     /translation="AYGQGLADEYEKTVKAEGLKVVSRDATNDKATDFKAILTKIKAE
                     RPDVIMYGGMDATGGPFAKQSMQLAIRSKIVAGDGVCTDDLVKLAEKATDNVICSIAG
                     GDLAKMPGGPAFAKKLKAAFGHDPVVYSPSSYDAVYVIVDAMKRAKSVKREDVLAALP
                     STNYNGVTGNIQFDEHGDLKGASISLYKYVSGKQTLLDVLKM"
     gene            812..1762
                     /locus_tag="VGC69_05780"
     CDS             812..1762
                     /locus_tag="VGC69_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0294829.1"
                     /translation="MDIFIQQLINGLVLGSVYAIIALGYTMVYGILGIINFAHGDVIM
                     VGAMVALSALNVLQNHFPGLGDIPMLLIALVIAAVVCAILGFAIERVAYRPLRNAPRL
                     APLITAIGVSILLETIAMVIWSPNPLPFPQLLPTDPIDVIPQTDTGIGAVISMTEIVI
                     IVVAFLVMAGLLVLVHKTRLGRAMRAIAENPNNASLMGVNPNFVISATFMIGSALAAL
                     AGLMMALEYGHVHFYMGFIPGMKAFTAAVLGGIGNLGGAMVGGIVLGLIEQLGAGYVG
                     DLTGGVFGSNYQDVFAFIVLILVLLFRPSGLLGERVADRA"
     gene            1791..2882
                     /locus_tag="VGC69_05785"
                     /pseudo
     CDS             1791..2882
                     /locus_tag="VGC69_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2912..3685
                     /locus_tag="VGC69_05790"
     CDS             2912..3685
                     /locus_tag="VGC69_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294830.1"
                     /translation="MSQDIRLFVKGVNKRFGGLQALSDVGLQIRSGEIYGLIGPNGAG
                     KTTFFNVITGLYTPDSGEFVLDGKTYTPQAVHQVTQAGIARTFQNIRLFGGMTALENV
                     MVGRHVRTRQGILGAVFRTQAERREEKEIRARAMELLDYVGISQYADYTSRNLSYGHQ
                     RRLEIARALATDPKLLALDEPAAGMNATEKVELTTLLDKIRQDGRTILLIEHDVKLVM
                     GLCNHMTVLDYGKIIAEGLPQDVQKDPKVIEAYLGAGGH"
     gene            3689..4405
                     /locus_tag="VGC69_05795"
     CDS             3689..4405
                     /locus_tag="VGC69_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294831.1"
                     /translation="MSETLLKISGLQVSYGGIQAVKGVDLEVGLGELVTLIGANGAGK
                     STTMRAITGLKAYSGGDIQYMGQSIKGVPAHELLARGLVMVPEGRGIFARMTIYENLL
                     MGAYKRKDKAGIEADIERMFGFFPRLKERQSQLAGTLSGGEQQMLAMSRAVLSQPKLL
                     LLDEPSMGLSPIMVDKIFEVVREISKEGITILLVEQNARLALQAANRGYVMDSGTVIM
                     TGDAQDMLHDPRVRAAYLGE"
     gene            4518..5129
                     /locus_tag="VGC69_05800"
     CDS             4518..5129
                     /locus_tag="VGC69_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY0294832.1"
                     /translation="MALHTWLLYLIAATGLSITPGPNTLLALTHGALHGHRKTLCTIS
                     GGALGFTALVALSMLGIGALIQAWAPALILLKWVGGAYLVWLGVHLWRAPGVQVRHDA
                     ATAAVVPGTRLFRQGFLSAASNPKALLFYGAFLPQFIDPQRGLLAQFAIMAATFVIIE
                     FLYEYLLARLAYRVQPWLARHGRRFNKTCGALFALFGIAMPTT"
     gene            complement(5164..5643)
                     /locus_tag="VGC69_05805"
     CDS             complement(5164..5643)
                     /locus_tag="VGC69_05805"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY0294833.1"
                     /translation="MRSLSSRTRLSVLLCSLLVPLAAAHAQDNPQRRQAEQQHREADQ
                     QRRQVDQQRRQVDQQRRQVDQQRRMDQARQRERDRVVTEQRRRDWEAERRRQYDNRRW
                     SQGQYLPRAYWDRRYYVPDWQVRHLPPPMVGYQWVYIDGQYVLMQIDTGLIVRILLP"
     gene            5887..7278
                     /locus_tag="VGC69_05810"
     CDS             5887..7278
                     /locus_tag="VGC69_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY0294834.1"
                     /translation="MSQASLETTPESAQRTGGLFSNEDWWSIWIGLALVVVAYGLFAS
                     GASIKWLTVAPTRWASLDQAWSDFAAHAGNYLALFAVFAVLFSIAIVTLKQRLAHFLP
                     SFLILFVVSIAIYYGGAWTSSVKYNLEPPLVALALGLIISNVFPLPEWFQAGLRVEFY
                     IKVGIILLGATLPFTLLVWAGPVAFGQATIVSLATFFTIFLVGRALGLDRRFAAVLGV
                     GGAVCGVSAAIAVSGAVRAKREQASSAITLVVLWAIVMIFVLPFASRSLGLSTGVAGA
                     WIGTSEFADAAGVAAAQAYGDLAKGNPTLIPGSPDAALQAFTLMKVVGRDIWIGIWAF
                     VLAIVATTSWERTDGVATKADAREIWTRFPKFVIGFVIASAFVTWVASHYSLADYRKI
                     VTPDLIAPITTLRVWAFTLTFLSIGLTTRVATLAKTGIRPFLAITAGVVVSLVIGYVL
                     SVYVFSSFWNALG"
     gene            7278..7505
                     /locus_tag="VGC69_05815"
     CDS             7278..7505
                     /locus_tag="VGC69_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294835.1"
                     /translation="MMSEPRCADCRHRQPDAGHVEKAVPGLTAFGSGYGASIGASRLC
                     LLHDCLVSPDDRCNAHAPWPADKLGKVPQAA"
     gene            7561..7634
                     /locus_tag="VGC69_05820"
     tRNA            7561..7634
                     /locus_tag="VGC69_05820"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7593..7595,aa:Cys,seq:gca)
     gene            7751..8143
                     /locus_tag="VGC69_05825"
     CDS             7751..8143
                     /locus_tag="VGC69_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294836.1"
                     /translation="MAENPFGDTDEQKAERRSYLLLIAASKDNAALVQQYHKNIQAHV
                     DEKASPLWVDSKGIGILISTHLVAAEIWREMFQTKDAIKEADTRDILIVEIGQDWAAR
                     RDAKTEHWLSTHVGTPRPAAPHERVRRR"
     gene            complement(8234..8764)
                     /gene="tsaA"
                     /locus_tag="VGC69_05830"
     CDS             complement(8234..8764)
                     /gene="tsaA"
                     /locus_tag="VGC69_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEY0294837.1"
                     /translation="MAVMHDAQRDEYIMVRLNEIRHNEVAVDSPAADDAGLEFIGRIR
                     TPWTNRLACPRMGRHDGPVCRIEVDEPWTAALDGVELFGRLEVLYWLHESRRDLVRQS
                     PANDGATRGTFSLRTPVRPNPIGTSIVELVSREGPVLLVRGLDCLDGTPLLDIKPDRD
                     LFTPIAPPQAGDFQTG"
     gene            complement(8755..>9300)
                     /locus_tag="VGC69_05835"
     CDS             complement(8755..>9300)
                     /locus_tag="VGC69_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0294838.1"
                     /translation="GLMFGASGILQNHRSVLKIDTPPPVVVNVRVQVPASVPQTPAGV
                     STWLQQELGLPKPFERVIRTPSQSVTWGDRTVQQPERWQLLFRTTREIIQAEYWPDAR
                     LVSARRSIPGWLGVIENLHRANGIGAAWVLLSDSIAGCLILLSLTGLLLWTELERKKL
                     VGTVVFVASVATTLVVTVSWP"
BASE COUNT         1583 a         3214 c         2841 g         1662 t
ORIGIN      
        1 gcctacggcc agggcctggc cgacgagtac gagaagaccg tcaaggccga aggcctcaag
       61 gtggtgtcgc gcgacgccac caacgacaag gccaccgact tcaaggccat cctgaccaag
      121 atcaaggccg agcgccccga cgtcatcatg tacggcggca tggacgccac cggcggcccg
      181 ttcgccaagc aatccatgca gctggccatc cgcagcaaga tcgtcgccgg cgacggcgtc
      241 tgcaccgacg acctggtcaa gctggccgag aaagccaccg acaacgtgat ctgctcgatc
      301 gccggcggcg acctggccaa gatgcccggc ggccccgcgt tcgccaagaa gctcaaggcc
      361 gccttcggcc acgaccccgt ggtgtactcg ccctcgtcgt acgacgccgt ctacgtgatc
      421 gtcgacgcca tgaagcgcgc caagtcggtc aagcgggaag acgtcctggc cgccctgccc
      481 tcgaccaact acaacggcgt cacgggcaac atccagttcg acgagcacgg cgacctgaag
      541 ggcgcgtcga tctcgctgta caagtacgtc tcgggcaagc aaaccctgct cgacgtcctg
      601 aagatgtaag gcagcgcatc aatgaggggc cgatcccggt gattggcccc tcttgctttt
      661 gccgcgtttt gcgcattttc ctcttacgtt tcaaaacccc gcccgagccc ttcgggcgtc
      721 gttttgttag gccgttccac aaggggacgg aacaacacac ccaaccccct gaatggaacc
      781 aggcatacca aaaatatgcc gccggagtca tatggacata ttcatccagc aactcatcaa
      841 cggactggtg ctaggcagcg tctacgccat catcgccctg ggctacacca tggtgtatgg
      901 catcctgggc atcatcaact tcgctcacgg cgacgtgatc atggttggcg ccatggtcgc
      961 cctgtcggcc ctgaacgtcc tgcaaaacca cttccccggc ctgggcgaca ttcccatgct
     1021 gctgatcgcc ctggtcatcg cggccgtcgt ctgcgccatc ctgggcttcg ccatcgagcg
     1081 cgtggcctac cggcccctgc gcaacgcgcc gcgcctggcg ccgctgatca ccgccatcgg
     1141 cgtgtcgatc ctgctggaga ccatcgccat ggtgatctgg tcgcccaacc ccctgccctt
     1201 cccgcaactg ctgcccacgg atccgatcga tgtcatcccg cagaccgaca ccggtatcgg
     1261 cgcggtgatc tcgatgaccg aaatcgtcat catcgtggtg gccttcctgg tcatggccgg
     1321 cctgctggtg ctggtccaca agacccggct ggggcgcgcc atgcgcgcca tcgccgagaa
     1381 ccccaacaac gccagcctga tgggcgtgaa tcccaacttc gtgatctcgg ccaccttcat
     1441 gatcggctcg gcgctggccg ccctggccgg cctgatgatg gccctggaat acggccacgt
     1501 gcacttctac atgggcttca tccccggcat gaaggccttc acggcggcgg tgctcggcgg
     1561 catcggcaac ctgggcggcg ccatggtggg cggcatcgtg ctgggcctga tcgaacagct
     1621 cggcgccggc tacgtcggcg acctcaccgg cggcgtgttc ggcagcaact accaagacgt
     1681 cttcgcgttc atcgtgctga tcctcgtgct gctcttcagg ccctccggcc tgctgggcga
     1741 acgtgtggcc gatcgcgctt aaggagcccc gcatgagttc caacaagaaa atcctgggca
     1801 tcgtcttcgc ggtgctggtc ctgctcgccc cgctgctcat cggcgccatg ggcggcaact
     1861 actgggtgcg cgtgctcgac ttcgccatgc tctacgtcat gctggccctg ggcctgaacg
     1921 tcgtggtggg cttcgccggc ctgctcgacc tgggctacat cgccttctat gccgtgggcg
     1981 cgtacacggc ggcgctgctg tcctcgccgc acctctccaa caacttcgaa tggatcgccc
     2041 acctggcccc gcacggcctg cacgcgccct tcctgctcat cgcggtgatc ggcatggcgc
     2101 tggcggccat cttcggcatc ctgctgggcg cgcccacgct gcgcctgcgc ggcgactacc
     2161 tggccatcgt gacgctgggc ttcggcgaga tcgtgcgcat cttcatgaac aacctgtaca
     2221 gcccggtcaa cttcaccaac gggccgcagg gcatcaacaa cgtgtcggcg gtgcacttcg
     2281 ggggcttcag cctggccgag acccactcgc tgctcggcat caccttcagc tcgacccagt
     2341 tgtactacta cctgttcctg atcggcgcgc tcttcgtgat gggcgtgtgc agccgcctgc
     2401 agggctcgcg catcggccgg gcctgggccg ccatccgcga ggacgaactg gccgccaagg
     2461 ccatgggcat caacacccgc aacatcaagc tgctggcctt cgccatgggc gcctccttcg
     2521 gcggcctgtc gggcaccatg ttcggcgcct tccagggatt cgtctctccc gagtccttca
     2581 ctttctggga atccatcgtc gtgctggcct gcgtggtgct gggcggcatg ggccacatcc
     2641 cgggcgtgat cctgggcgcc gtgctgctgt cggtcttccc cgaagtgctg cgcgtcaccg
     2701 tgggccccat ccagaacgtc ctcttcggcc atgaaatcgt cagcgcctcc atcatccgcc
     2761 agggcgtcta cggcctggcc atggtgctca tcatgctgta ccgccaaaag ggcctgtggc
     2821 ccgcgcccac gcatgaagac cacgtcgcca ggctcgccaa gagcggcaaa gcgacggcat
     2881 aaggccacag actcacaggt actcgaaaaa catgagccaa gacatccgac ttttcgtcaa
     2941 gggcgtgaac aagcgctttg gcggcctcca ggcgctctcc gacgtgggtc tgcagatccg
     3001 gtccggcgag atctacggcc tcatcggccc caacggcgcc ggcaagacga ccttcttcaa
     3061 cgtcatcacc ggcctctaca cgcccgactc gggcgagttc gtgctcgacg gcaagaccta
     3121 cacgccccag gccgtgcacc aggtcaccca ggccggcatc gcccgcacct tccagaacat
     3181 ccgcctgttc ggcggcatga ccgccctgga aaacgtgatg gtgggccgcc acgtgcgcac
     3241 gcgccagggc atcctgggcg cggtcttccg cacccaggcc gagcgccgcg aggaaaagga
     3301 aatccgcgcg cgcgccatgg agctgctcga ctacgtgggc atctcgcaat acgccgacta
     3361 cacgtcgcgc aatctttcct acggccacca gcgccgcctg gagatcgccc gcgccctggc
     3421 caccgacccc aagctgctgg cgctcgacga gccggccgcc ggcatgaacg ccaccgagaa
     3481 ggtggagctc accacgctgc tcgacaagat ccgccaggac ggccgcacca tcctgctgat
     3541 cgagcacgac gtcaagctgg tcatgggcct gtgcaatcac atgaccgtgc tggactacgg
     3601 aaaaatcatc gctgaaggac tcccgcaaga cgtgcagaag gatcccaagg tcatcgaagc
     3661 ctatctgggc gcgggaggtc actgaaccat gtccgaaact ctcctcaaaa tctcgggcct
     3721 gcaggtcagc tacggcggca tccaggccgt caagggcgtc gacctcgaag tgggcctggg
     3781 cgaactggtg acgctgatcg gcgccaacgg cgccggcaag agcaccacca tgcgcgccat
     3841 caccggcctg aaggcctatt cgggcggcga catccagtac atgggccagt cgatcaaggg
     3901 cgtgcccgcg cacgaactgc tggcgcgcgg cctggtcatg gtgccggaag gccgcggcat
     3961 cttcgcccgc atgaccatct acgaaaacct gctgatgggc gcctacaagc gcaaggacaa
     4021 ggccggcatc gaggccgaca tcgagcgcat gttcggcttc ttcccgcgcc tgaaggagcg
     4081 ccagtcccag ttggccggca cgctctcggg cggcgagcag cagatgctgg ccatgtcgcg
     4141 cgccgtgctc agccagccca agctgctgct gctcgacgaa ccctccatgg gcctgtcgcc
     4201 catcatggtc gacaagatct tcgaagtggt gcgcgagata tccaaggaag gcatcaccat
     4261 cctgctggtc gagcagaacg cccgcctggc cctgcaggcc gccaaccgcg gctacgtgat
     4321 ggactcgggc accgtcatca tgacgggcga cgcgcaggac atgctgcacg acccccgcgt
     4381 gcgcgcggcc tatctgggcg agtagaccgg gccgcctatc gcgccggccg caacggccat
     4441 ggccagtccg gccggcacgc agggcaatga gctaagcttg ttgccctgtt ttcattttcg
     4501 gcattcgagc ggctcccatg gcattgcaca cctggctcct gtacctgatc gcggcgaccg
     4561 gcctgtccat cacgcccggc cccaataccc tgctggcgct cacccacggc gccctgcacg
     4621 gacaccgcaa gacgctgtgc acgatcagcg gcggcgcgct gggctttacg gcgctggtcg
     4681 cgctgtccat gctgggcatc ggcgccctga tccaggcctg ggcgccggcg ctcatcctcc
     4741 tcaagtgggt gggcggcgcc tatctggtgt ggctgggcgt gcatctgtgg cgcgcgcccg
     4801 gcgtgcaagt gcggcacgat gccgcaaccg ccgccgtggt gccgggtacc cgcctgttcc
     4861 gccagggctt cctgtcggcg gcctccaacc ccaaggcgct gttgttctac ggcgccttcc
     4921 tgccgcagtt catcgatccg caacgcggcc tgctggcgca attcgcgatc atggcggcca
     4981 ccttcgtcat catcgaattc ctctacgaat acctgctggc ccgcctggcc taccgcgtgc
     5041 agccctggct ggcacgccac ggccgccgct tcaacaagac ctgcggcgcg ctcttcgccc
     5101 tgttcggcat cgccatgccc accacctgac gcccgccggg cgaaccgcgg cgggcaaccg
     5161 gcatcagggc agcaggatgc ggacgatcag cccggtgtcg atctgcatca gcacgtactg
     5221 cccgtcgata tagacccact gatagcccac catgggcggc ggcagatggc ggacctgcca
     5281 atcgggcacg taataccggc gatcccagta ggcgcgcggc aggtactggc cctgcgacca
     5341 acggcggttg tcatactggc ggcggcgttc ggcctcccag tcgcggcggc gctgctcggt
     5401 gaccacccgg tcgcgctctc gctggcgcgc ctggtccatg cggcgctgct ggtcgacctg
     5461 gcgacgctgc tggtcgacct gcctgcgctg ctggtccacc tgccggcgct gctgatcggc
     5521 ttcgcgatgc tgctgctcgg cctggcggcg ctggggattg tcctgggcat gcgcggcggc
     5581 caatggcacc agcagcgagc acaacaggac ggacaaacgg gtacgggaag aaagggagcg
     5641 catggtgact cctgaaaaac agcctacagc tgcgttgctg ttacagataa cagaccaggg
     5701 acgccttgca aagtctccca ccgtccggtg aattccaaat gattacaaat ctggaatgct
     5761 gcgttcctta tatcgcaaag caataaccat tgaagaacac aatcgttctc taactgggga
     5821 ggtatccaga taatcgctcg atggcccccg cggaaaaagg gccgcaaccg ataagaggaa
     5881 aatatcatgt cccaagcaag cctcgagacc acgcccgagt ccgcccagcg caccggcggg
     5941 ctgttctcga acgaagactg gtggtcgatc tggataggcc tggccctggt cgtcgtggcc
     6001 tacggcctct tcgcctcggg cgccagcatc aagtggctga ccgtggcccc gacgcgctgg
     6061 gcttcgctcg accaggcctg gagcgacttc gccgcgcatg ccggcaatta tctggcgctc
     6121 ttcgcggtgt tcgccgtgtt gttctctatc gccatcgtca cgctcaagca gcgcctggcg
     6181 cacttcctgc cgtcgttcct gattctgttc gtggtgtcga tcgcgatcta ctacggcggc
     6241 gcctggacca gctcggtcaa gtacaacctc gagccgccgc tggtggcgct ggcgctgggc
     6301 ctgatcattt ccaacgtgtt tccgctgccg gaatggttcc aggccggctt gcgcgtggag
     6361 ttctacatca aggtgggcat catcctgctg ggggccaccc tgcccttcac gctgctggtc
     6421 tgggccggcc cggtcgcgtt cggccaggcc accatcgtgt cgctggccac cttcttcacc
     6481 attttcctgg tcggccgcgc cctgggactg gaccggcgct ttgccgccgt gctgggcgtg
     6541 ggcggcgcgg tgtgcggcgt atcggccgcc attgcggttt ccggcgcggt gcgcgccaag
     6601 cgtgaacagg cctcgtcggc gatcaccctg gtcgtcctgt gggccatcgt catgatcttc
     6661 gtcctgccct tcgcttcgcg ttcgctgggc ctgtccaccg gcgtcgccgg cgcctggata
     6721 gggacctcgg aattcgccga tgccgccggc gtggccgcgg cccaggccta tggcgatctg
     6781 gccaagggca atcccactct cattcccggc tcgcccgacg ccgccctgca ggcctttacg
     6841 ctgatgaagg tcgtgggccg cgacatctgg atcggcatct gggccttcgt gctcgccatc
     6901 gtcgccacca cgagctggga gcgcaccgac ggcgtggcga ccaaggccga tgcgcgcgaa
     6961 atctggacgc gctttcccaa gttcgtcatc ggctttgtca ttgcctcggc tttcgtcacc
     7021 tgggtcgcca gccattattc gctggccgac taccgcaaga tcgtcacccc ggacctgatc
     7081 gccccgatca ccacgttgcg cgtctgggcc ttcaccttga ccttcctctc catcgggttg
     7141 acgacgcgcg tcgccacgct cgccaagacc ggcatcaggc ctttcctggc catcaccgcc
     7201 ggcgtggtcg tcagcctggt catcggctac gtgctctcgg tgtacgtgtt ctcgtcgttc
     7261 tggaacgcgc tgggataatg atgtccgaac ctcgttgcgc cgactgccgg caccgtcagc
     7321 cggacgccgg ccatgtggaa aaagccgtgc ccgggctgac ggccttcggc tcgggctacg
     7381 gcgccagcat cggcgccagc cggctgtgcc tgctgcatga ttgcctggtg tcgccggacg
     7441 atcgctgcaa tgcccatgcg ccatggccgg cggacaagct cgggaaggtg ccgcaggcag
     7501 cctgaggggc aacgccaggg caaccccctc gggtttgttg tatagttgag ggcttgccgg
     7561 ggcgcaatgg cagagtggtc atgcagcgga ttgcaaatcc gtgtacgtcg gttcgattcc
     7621 ggcttgcgcc tccagcgaaa tcaagggttt agctgcaata gccaggctct ttcccccgga
     7681 actgcgacag cacgctgcga catcgtggga tcatccatgt gtgctgcaac ccgcccccag
     7741 ggaactgatc atggctgaaa atccgtttgg cgatactgac gagcaaaaag ctgagcgccg
     7801 ctcctacctg ctactgatcg ccgcctcgaa agacaatgcg gcgcttgtcc agcaatacca
     7861 caagaacatt caagcgcacg tcgatgaaaa ggcgtcgccg ctttgggtcg attccaaagg
     7921 cataggcatc ctgatatcga cccatcttgt cgctgcggaa atctggcgtg agatgtttca
     7981 aaccaaggac gctatcaagg aagccgacac ccgggacatc ttgatcgttg aaatcggcca
     8041 ggactgggcg gcacgtcgtg atgccaaaac agagcattgg ctgtctaccc atgtcggcac
     8101 cccacgcccc gccgctccgc acgaacgggt tcgacgccgt tgatgccctg cgtcccaagc
     8161 atccggcccg ccgccttggt gggcgttttg tttttccggc gttgccccaa agagcttcga
     8221 aagcccccgc aaatcagcca gtctgaaaat cccccgcctg cggcggcgcg atgggagtga
     8281 acaagtcacg gtcgggcttg atatccagga gcggcgttcc atccaggcaa tcgaggcctc
     8341 tcactagcag caccgggcct tcccgggaca ccagctcgac gatggacgtg ccgatgggat
     8401 tgggccgcac gggggtgcgc agcgagaacg tgccgcgcgt cgcgccatcg ttggcggggc
     8461 tttggcggac gagatcgcgc cgcgactcgt gcagccagta gagcacctcc agccgtccga
     8521 acaattcgac gccgtcaagc gcggccgtcc agggctcatc gacctcgatg cggcagaccg
     8581 ggccgtcatg acggcccatg cgcggacacg ccagcctgtt tgtccacggc gtcctgatcc
     8641 tgccgatgaa ttcgagcccc gcgtcgtccg cggccgggga atccacagcg acttcattgt
     8701 ggcgaatctc gttcaaccga accatgatgt attcatcccg ctgggcgtca tgcattacgg
     8761 ccatgatacc gtcaccacca gcgtcgtcgc caccgacgcg acgaacacca ccgtgcccac
     8821 cagcttcttg cgctcgagct ccgtccacag cagcaggccg gtcagcgaca acaggatcag
     8881 gcagccggcg atggagtccg acagcagcac ccacgccgcg ccgatgccgt tggcccgatg
     8941 caagttctcg atgacgccca accagccggg tatgctgcgg cgggccgaga ccaggcgggc
     9001 atcgggccag tattcggcct ggatgatttc ccgggtcgtg cgaaacagca gttgccagcg
     9061 ttcgggctgc tgcacggtgc gatcgcccca ggtcacggac tggctgggcg tgcgtatgac
     9121 acgctcgaag ggtttgggca ggcccagctc ctgctgcagc caggtcgaca cgcctgcggg
     9181 cgtctggggc accgaggccg gcacctgcac gcgcacattg acgacgacgg gtggcggcgt
     9241 atcgatcttc agcaccgaac ggtgattctg gagaatgccg cttgcgccga acatcaggcc
//
LOCUS       DASWPO010000095        12202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_109888, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000095 DASWPO010000000
VERSION     DASWPO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109888"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGC69_05840"
     CDS             <1..376
                     /locus_tag="VGC69_05840"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY0294839.1"
                     /translation="HPIIMGRKTWESLGRPLPGRTNIVVTRQPGYAAEGAVVTSSLQA
                     ALDACLDEKAPYVIGGAQIYAQALPLADRVVATEVHTEAVGDAWFPTLPPAQWRETDR
                     QPQAEENGLQYDFVTYERILPA"
     gene            complement(506..1561)
                     /locus_tag="VGC69_05845"
     CDS             complement(506..1561)
                     /locus_tag="VGC69_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEY0294840.1"
                     /translation="MKKSAVVGGVVVVLVAAWLGATWYTGKRLEAEAPARLDELNQRL
                     AQSLPDYGLKFEQISFERGFFSSHARYGLTMVGKGRTPDQPAVPAGVLQFDAVAYHGP
                     LPLQALAHGSLMPQLAWVHGEVVQTDGLKDVFEATHGKTPLWSDTAFSYGGDTHGTGG
                     VAPFDFVFQGHKAAFGGMQVQSSYTRSTQGLQSTMVFPSLTLEGADLGNGQLSIKTDM
                     QSLVIDPLVWKNDKGEGRAVVTVTGQVPPPGAQGDAAKPVIQSVSARVVLSKPMLGEL
                     GSRYLAAMQGMSPQDADHQAARQLAGLSGVGQMLGLVRNDGDNLVSELQYANGKLSLN
                     GRERDVGQVGRMVPGIP"
     gene            complement(1576..3069)
                     /locus_tag="VGC69_05850"
     CDS             complement(1576..3069)
                     /locus_tag="VGC69_05850"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0294841.1"
                     /translation="MRQLTHYINGMAVEGRSGRYSDGFDPSTGEITRAVPLADASEVD
                     AAVAAAQAAFPAWSETPPLKRARILFNFKALIDKHQNELAEIITREHGKVFSDALGEV
                     TRGLEVVEFACGIPHLLKGQYTDQIGGGIDNWSMRQSLGVVAGVTPFNFPMMVPCWMF
                     PVALACGNTFVLKPSERDPSCAIRLAELLKEAGLPDGVFNVVHGDKAAVDALIAHPKV
                     EALSFVGSTPIAEYIYAEGTKRGKRVQALGGAKNHLVVMPDANLDQVTDALMGAAYGS
                     AGERCMAISVAVAVGDVADKVVERLVPRVKSLVVKNGMEPDAEMGPLVTAQHRAKVMG
                     YIEDGVAVGAKMLVDGRGLTVPGHENGFFLGGTLFDHVQPNMNIYREEIFGPVLCVVR
                     VPDFASAVELINAHEFGNGVSCFTSDGGIARAFSRQIKVGMVGINVPIPVPMAWHSFG
                     GWKRSLFGDHHAYGEEGVRFYTRYKSIMQRWPDSIGKGAEFTMPVAK"
     gene            complement(3177..4463)
                     /locus_tag="VGC69_05855"
     CDS             complement(3177..4463)
                     /locus_tag="VGC69_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0294842.1"
                     /translation="MPFTANKQFKAQPRMLAGASGMYYTSTDGRQILDGTAGLWCVNA
                     GHCRTEIVQAIAKQAAEMDYAPGFQMGHPIAFEAATAVAGIMPKGLDRIFFTNSGSEA
                     IDTALKIALAYHRARGEGHRTRLIGRERGYHGVGFGGISVGGISPNRKAYANSLLPGV
                     DHIVHTHNLEKNAFSKGQPAWGAELADDLERVIALHDASTIAAVIVEPMAGSTGVLIP
                     PKGYLERLREITSKHGILLIFDEVITGFGRLGAATAAERFGVTPDLLTMAKGISNAAV
                     PCGAVAVRRDVHDAIVNATASGIELFHGYTYSGHPLAMAAVVATLDLYRREGLFKRAA
                     ELAPTFEAAVHGLKGARHVVDVRNLGLVAGIELKSRDGAVGARAAEVFQKCFAEEDLM
                     VRYTGEILAVSPPLIISEAQIADLFGRIGKVLATVD"
     gene            4682..6112
                     /locus_tag="VGC69_05860"
     CDS             4682..6112
                     /locus_tag="VGC69_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0294843.1"
                     /translation="MIDFQPDRSRNGANTLVDQIVDAYARAIAGQALRPGMSVPSVRA
                     FAREHGISAFTVAGAYARLAAQGWLAARPGSGYRVAAGGTSAASRSAAPAAAWQPPRL
                     GAGWLLSDIYADHSIPIKAGCGWLPGEWLNEEGLHQALRHMGRVPALRISGYGHPYGH
                     APLRETIAAGLAAHGLQAAADQVVLTQGVTHGLDLVIRTLLRPGDTVLVEQPCYANLL
                     PLLRLAGLRVLAVSRDAQGLDCDALDRLAREHAPRAVFVNTVLQNPSGTTLGMANAFR
                     LLQLAQQHEMWVVEDDISRELLPGVAPLLAALDGLQRVVYLGGYSKVISPSVRVGYIV
                     AHRDLMRDIARTKMAVGLTSPEIMERVVHQIIREGRYRGHILRTRERLAQAHAEVEAR
                     MDESGLEIAARPQAGLFLWAKPSGMQGANALAQRALEDGIWLAPGSYFDVGEADVPWV
                     RFNVAYSTGAELWKFLAHPDRPSTPG"
     gene            6191..6940
                     /locus_tag="VGC69_05865"
     CDS             6191..6940
                     /locus_tag="VGC69_05865"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF014650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HEY0294844.1"
                     /translation="MAPRRFIQASAFLAIAVALLYGCQARVHNPDVLWQIVSQSCVPS
                     AEAGKGPGKCAKVSPDGYAILKDINGKGQHLLIPTTRVTGVEDPLLLRSGSPDYFRYA
                     WEDRDFVSKALGARVPDELMSLALNSADGRTQNQFHIHVDCLSQDMRNALSAYDGATT
                     GAWLNTTFNGHPYRLERVAAATAAGVDPFGLVLRQAAAAQQAMRGHTIFMASAPGGFF
                     VVDGYYQPYGPDANPGSSEELQDHSCKDVRK"
     gene            complement(6943..7323)
                     /locus_tag="VGC69_05870"
     CDS             complement(6943..7323)
                     /locus_tag="VGC69_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY0294845.1"
                     /translation="MEKIRLDKWLWAARFYKTRSIAVDEIGKGRVHVNGVVGKPSREV
                     GPGDLVSIRKEDLVFHVEVRGVSGMRGPAPVARALYEETPESIKARERAAALRRLAPE
                     PAQEIREGRPTKKDRRLIDDWRGR"
     gene            7479..8249
                     /locus_tag="VGC69_05875"
     CDS             7479..8249
                     /locus_tag="VGC69_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0294846.1"
                     /translation="MAKILPVARTLAPEGISQEEWEIRCDMAAMFRAMARLGMNEQIA
                     NHCSMMLPGSDNLFLINPRGYHYSELTAGLLIVCDLDGNVVRGEGELRKVAFHIHARL
                     HIRHPQARCILHVHPQYLTALSMLEKGRMELVHQNSAALYDRVAYDEVHNGVVLWDEE
                     GDRIADVLGERTILVMKNHGVTVVGPSVAEAFDELYIAERTCMYQMTAQNTGLAMQTI
                     ADDLRDRYNGPWGERFDARLHLDAWRRIFNREEPEYAA"
     gene            8293..9840
                     /locus_tag="VGC69_05880"
     CDS             8293..9840
                     /locus_tag="VGC69_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294847.1"
                     /translation="MKRIFLSASLAAAVCATAFGPATAWAQKRGGDVVVAQQAQPPSL
                     DAQITFAQASRNITLHIYEALFARNDKGETIPDLAESYATAPDGLSYEIKLRHGVKFH
                     NLKEMTATDVKASLERYAKYGASAQNMALVDHIDIVDPYTLKIAMKSPFQGFIEFLGS
                     PRAPVAIYPAEEAAKGPNQIDIIGTGPYQLVEYKPDSYVKLKRFEAYSANMNYPGRDG
                     LGGRKTAYLDTVTFRFMPEAGARESALQTGEVQVLEAISATAAKRLKNDPNIKTYEMM
                     PWAFQTIFLNAGAPPTDNLKVRQAIQAALDDEEIMAISTDGLYRMTHGWQHPGTPYYA
                     GDVGKNLYNQHNLDRARQLLKEAGYKGEELTFIADNGFKNHNDTAVIATQQLQAIGMN
                     VKLKIADWPTTLAAREKPTGWNLFPIMVGIEPYEGPNGVAVFFTGDKNWQNHKDAGME
                     AAMLKLQTAPALSDRQAAFATIQQIIYDQVYAIKVGDIGVLEAARANLKNFVPHRIPR
                     MWDVWYE"
     gene            9869..10816
                     /locus_tag="VGC69_05885"
     CDS             9869..10816
                     /locus_tag="VGC69_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294848.1"
                     /translation="MLRYLIGRLLGAVPVLLLVSLLTLSLLWLVPGDAAAELAGPGAS
                     TEELQRLRTELGLDKPVYIQAARWYGNVLHGDLGRSLLLNRGVTEAILERLPVTGSLT
                     LLALFMTVAIGMASGVLAAVRRNTWVDQLSMSLALLGLSLPDFWLGLVGIYLFSVTLG
                     WFPTGGYVPFSDSPIGWLHSLFLPAAALAATQLGLLARMTRSSMLEVLAQDYIRTAKS
                     KGLPPRLVVGKHALANIAIPTLTVIGVNAGILIGGAVIVESVFSIPGVGRLVIGAIQR
                     RDLPVIQGGLLLLACVMVLINLLVDILYAVFDPRVRHGR"
     gene            10806..11681
                     /locus_tag="VGC69_05890"
     CDS             10806..11681
                     /locus_tag="VGC69_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294849.1"
                     /translation="MAGNVLASDGRAGGWPLAARLSRHRLFMTGLCLLLAIVLLSLLA
                     GWLAPYSPVANDYRYRLGAPNAAHWMGTDSFGRDVFSRMLYGSRVSLRIGLFVMLCTG
                     IIGTAIGLCAGYFRRLEGPIMRVMDALMAFPGILLAIALASVLGPSEANVVLALTVTY
                     TPRTARIMRAAALVLSEMEYVQAAKAAGAGHLRILVRHIAANSMAPLIVQMTFIFAIS
                     ILAEAVLSFIGVGPPPPSPTWGNIIADGRDFIPDAPWVCLFPGLAVAMAVLGLNLIGD
                     GLRDVLDPRLRTDLR"
     gene            11678..>12202
                     /locus_tag="VGC69_05895"
     CDS             11678..>12202
                     /locus_tag="VGC69_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294850.1"
                     /translation="MNGVSPPNHAAPLLDVQNLSTVVQAGGRRLTVLDDISFDIRPGE
                     ALALVGESGSGKSMAALSIMRLLAAPARVASGRALFKGADLFSLSESQMRGIRGASIS
                     MIFQEPMSSLNPVFTVGDQIGEVLRYHKGINRRAARRQAIGLLDLVGIPAAAQRVDNY
                     PHQMSGGMRQRVMIA"
BASE COUNT         2071 a         4318 c         3861 g         1952 t
ORIGIN      
        1 ccaccccatc atcatgggcc gcaagacctg ggaatcgctg ggccggccgc tgccgggccg
       61 caccaatatc gtggtcacgc gccagcccgg ctacgcggcc gaaggcgccg tggtgacaag
      121 ctcgctgcag gcagcgctgg acgcctgcct ggacgagaaa gccccctacg tgatcggcgg
      181 cgcgcaaatc tacgcccagg ccctgcccct ggccgatcgc gtcgtggcca ccgaagtgca
      241 caccgaagca gtcggcgatg cgtggtttcc cacgctgccg cccgcccaat ggcgggaaac
      301 cgaccggcag ccgcaagccg aggaaaacgg cctgcaatac gacttcgtga cctacgaacg
      361 catcctgccc gcctgagacg ccttgagccg aggaccccgg agcaatccga agcacagccc
      421 cttttcaggg gcgagcatcg gaattgcgca gggcagcctc ggcgcctcaa gaactcaaaa
      481 aatcagtacc cagtagcaat aacgctcacg gaatcccagg caccatccgc cccacctgcc
      541 ccacatcccg ctcgcgccca ttgagcgaca gcttgccgtt ggcatactgc agttcgctca
      601 ccaggttgtc gccatcattg cgcaccagcc ccagcatctg ccccacgccg gacaagccgg
      661 ccaactggcg cgccgcctgg tggtcggcgt cctgcgggct catgccctgc atcgcggcca
      721 gatagcggga acccagctcg cccagcatgg gcttggacag caccaccctg gccgacaccg
      781 actggataac cggcttggcc gcatcgccct gcgccccagg cggagggacc tggcccgtca
      841 ccgtcaccac ggcccggccc tcgcccttgt cgttcttcca gaccagcgga tcgatgacca
      901 gcgattgcat atccgtcttg atcgagagct ggccattgcc caggtcggcg ccttccagcg
      961 tcagcgaagg aaacaccatc gtgctctgca ggccctgcgt cgaacgggta tagctgctct
     1021 gcacctgcat gccgccgaac gcggccttat gcccctggaa cacgaaatcg aagggcgcga
     1081 cgccgcccgt gccatgggtg tcgccgccgt acgagaacgc ggtgtcgctc cacagcggcg
     1141 tcttgccatg cgtggcctcg aaaacgtcct tcagcccatc cgtctgcacc acctcgccat
     1201 gcacccaggc cagctgcggc atcaggctgc catgcgccag cgcctgcagg ggcaaggggc
     1261 cgtggtaggc caccgcgtcg aactgcagca cgccggcggg cacggccggc tggtcgggcg
     1321 tcctgccctt gcccaccatg gtcagcccgt agcgcgcatg cgaagagaaa aagccgcgct
     1381 cgaagctgat ctgctcgaac ttcaacccat agtcgggcaa agactgggcc agccgctggt
     1441 tcagttcgtc cagccgcgcc ggcgcctcgg cctcgaggcg tttgccggta taccaggtgg
     1501 cgcccagcca ggctgcgacc agcaccacga ccacgccgcc gacaactgcg gattttttca
     1561 ttgcgacgga ttcccttact tggccaccgg catggtgaac tccgccccct tgccgatgct
     1621 gtctggccag cgctgcatga tgctcttgta gcgcgtgtag aaacgcacgc cctcttcgcc
     1681 gtaggcgtgg tggtcgccga acagcgagcg cttccagccg ccgaaggaat gccaggccat
     1741 gggcaccggg atgggcacgt tgatgcccac catgcccacc ttgatctggc gcgagaaggc
     1801 ccgcgcaatg ccgccgtcgg aagtaaagca cgacacgccg ttgccgaact cgtgggcgtt
     1861 gatcagctcc accgcgctgg cgaagtcggg cacgcgcacc acgcacagca ccgggccgaa
     1921 aatctcttcg cggtagatgt tcatattggg ctgcacatgg tcgaacagcg tgccgcccag
     1981 gaagaagccg ttctcgtggc ccggcaccgt caggccgcgg ccgtcgacca gcatcttggc
     2041 gcccacggcc acgccgtcct cgatatagcc catgaccttg gcgcgatgct gcgccgtcac
     2101 cagcggaccc atctcggcgt cgggctccat gccgttcttg accaccagcg acttcacgcg
     2161 cggcaccagg cgttcgacca ccttgtcggc cacgtcgccc accgccacgg ccaccgagat
     2221 cgccatgcag cgctcgccgg ccgaaccata ggccgcgccc atcagcgcat cggtcacctg
     2281 gtcgaggttg gcgtcgggca tgaccaccag gtggttcttg gcgccgccca gggcctgcac
     2341 ccgcttgccg cgcttcgtgc cctcggcgta gatgtattcg gcgatcggcg tcgagcccac
     2401 gaaggacagg gcctcgacct tgggatgggc gatcagcgcg tccaccgcgg ccttgtcgcc
     2461 gtgcaccaca ttgaacacgc cgtcgggcag gccggcttcc ttcagcagct cggccaggcg
     2521 gatggcgcac gagggatcgc gctcggaagg cttgagcaca aaggtgttgc cgcaggccag
     2581 cgccaccggg aacatccagc acggcaccat catcgggaag ttgaagggcg tgacgcccgc
     2641 caccacgccc agcgactggc gcatgctcca gttgtcgatg ccgccgccga tctggtcggt
     2701 gtactggccc ttgagcaggt gcgggatgcc gcaggcgaac tccaccactt ccaggccgcg
     2761 cgtgacttcg cccagcgcgt cggagaacac cttgccatgc tcgcgcgtga tgatctcggc
     2821 cagttcgttc tggtgcttgt cgatcagcgc cttgaaattg aacaggatgc gcgcgcgctt
     2881 gagcggcggc gtttccgacc aggcgggaaa ggccgcctgc gcagccgcca ccgcagcgtc
     2941 gacttcggag gcgtcagcca gcggcacggc gcgcgtgatc tcccccgtcg acgggtcgaa
     3001 cccgtcgctg tagcggccgc tgcggccttc gaccgccata ccgttgatgt aatgagtgag
     3061 ctgtctcatg atgtttttct cgtcatggaa aatgaatgcg gtaacgggga gcataacgcg
     3121 taatcctcca cgcgccatgc tccccgtcgt ctattaatga cagggcgccg tccggctcaa
     3181 tccaccgtgg ccagcacctt gccgatgcgt ccgaacagat cggcgatctg cgcctcggag
     3241 atgatgagcg gcggcgacac cgccaggatc tcgccggtgt agcgcaccat caggtcctct
     3301 tcggcgaagc acttctggaa cacttcggcg gcgcgcgcgc ccaccgcgcc gtcgcgcgac
     3361 ttgagctcga tgccggccac caggcccagg ttgcgcacgt ccaccacatg cctggcgccc
     3421 ttgaggccgt gcacggcggc ctcgaaagtg ggggccagtt cggcggcgcg cttgaacagg
     3481 ccttcgcgac gatagaggtc cagcgtggcg accacggccg ccatggccag cggatggccc
     3541 gaataggtat agccgtggaa cagttcgatg ccgctggccg tggcattgac gatggcatcg
     3601 tgcacgtcgc ggcgcaccgc caccgcgccg cagggcacgg cggcgttgct gatgcccttg
     3661 gccatggtga gcaggtcggg cgtgacgccg aagcgttcgg cggccgtggc cgcgcccagg
     3721 cgcccgaaac ccgtgatcac ttcatcgaaa atcagcagga tgccgtgctt ggacgtgatc
     3781 tcgcgcaggc gctccagata gcccttgggc ggaatcagca cgccggtcga acccgccatg
     3841 ggctcgacga tcacggcggc gatggtcgag gcgtcgtgca gcgcgatcac gcgctccagg
     3901 tcgtcggcca gctccgcgcc ccaggccggc tggcccttgg agaacgcatt cttttccagg
     3961 ttgtgggtat ggacgatgtg gtccacgccg ggcagcagcg agttggcgta ggccttgcgg
     4021 ttgggggaga tgccgcccac cgagatgccg ccgaaaccca cgccgtggta gccgcgctcg
     4081 cggccgatca ggcgcgtgcg atgcccttcg ccgcgggcgc gatggtaggc cagcgcgatc
     4141 ttcagcgcgg tgtcgatcgc ctccgaaccc gagttggtga agaagatgcg gtccaggccc
     4201 ttgggcatga tcccggccac ggccgtcgcc gcttcgaagg cgatgggatg gcccatctgg
     4261 aagcccggcg cgtagtccat ttcagccgcc tgcttggcga tggcctgcac gatctccgtg
     4321 cggcagtggc cggcgttcac gcaccacagg cccgccgtgc cgtccaggat ctggcggccg
     4381 tcggtcgagg tgtagtacat gccggaggcg ccggccagca tgcgcggctg cgccttgaac
     4441 tgcttgttgg cagtgaaagg catccacaag tgggagagat cgggcaggtc ggccatggcg
     4501 ggctccagaa gcaaaggaaa gggcaggcgc gagcggcttg cccaaggaaa ggggatgcat
     4561 gaattctgat cccggccgga gagcctgaac atatacaatc cgaacaaatg acactaactg
     4621 tattggcaat acaatactta ccgtacagat acatgcctgc tgcgtcctgg catcgagccc
     4681 catgatcgac ttccagcccg accgatcccg aaacggggcc aatacacttg tcgaccagat
     4741 tgtcgatgcc tatgcccgcg ccatcgccgg gcaggcgctg cgccccggca tgtccgtccc
     4801 ctcggtgcgg gccttcgccc gcgagcacgg catcagcgcc tttaccgtgg ccggcgccta
     4861 tgcgcggctg gcggcgcaag gatggctggc ggcgcggccc gggtcgggat accgggtcgc
     4921 ggcgggcggg acaagcgcgg cttcccgctc cgccgcgccc gccgccgcct ggcaaccgcc
     4981 caggctcggc gcgggctggc tgctgtcgga catctacgcc gaccactcca tccccatcaa
     5041 ggccggctgc ggctggctgc ccggcgaatg gctcaacgag gaaggcctgc accaggccct
     5101 gcgccacatg ggccgcgtgc cggcgctgcg catctcgggc tacgggcacc cctacggcca
     5161 cgcgcccttg cgcgagacca tcgccgccgg cctggcggcc cacggcctgc aggccgccgc
     5221 cgaccaggtc gtgctgaccc agggcgtcac gcacgggctg gacctcgtca tccgcacctt
     5281 gctgcggccc ggcgacacgg tgctggtcga acagccctgc tacgccaacc tgctgcccct
     5341 gctgcgcctg gccggcctgc gcgtgctggc ggtatcgcgc gacgcgcaag ggctggactg
     5401 cgatgcgctg gatcgccttg cccgcgaaca cgcgccgcgc gcggtcttcg tcaacaccgt
     5461 gctgcaaaac cccagcggta cgaccttggg catggccaat gccttccgcc tgctgcagct
     5521 cgcgcagcag cacgaaatgt gggtggtgga agacgacatt tcgcgcgagc tgctgccagg
     5581 cgtcgcgccg ctcctggctg cgctcgacgg cctgcagcgc gtggtctacc tgggcggcta
     5641 ttccaaggtc atcagcccgt cggtgcgcgt gggctacatc gtggcgcacc gcgacctgat
     5701 gcgcgacatc gcgcgcacca agatggcggt gggactgacc tcgccggaga tcatggagcg
     5761 ggtggtgcac cagatcatcc gcgaaggccg gtatcgcggg cacatcctgc gcacgcggga
     5821 aaggctggcg caggcgcacg ccgaggtcga ggcgcggatg gatgagtcgg ggctggagat
     5881 tgcggcgcgg ccgcaggcgg ggttgttttt atgggccaag ccaagcggca tgcaaggtgc
     5941 caatgctctg gcgcagcggg cgctggaaga cgggatctgg ctggcgccgg gatcctattt
     6001 cgacgtgggc gaagcggacg tgccctgggt gcggttcaac gtggcgtatt cgacgggggc
     6061 tgaactatgg aagtttcttg cccatcccga tcgcccaagc acacccggtt gaccatgcct
     6121 gggtaatatg ccggcaatac cggcgctcgt acactgccct gcataagaca gacctattcc
     6181 ggacactcac atggcgccac gacgttttat ccaagcctcg gctttcctcg ccatcgccgt
     6241 cgccctgctc tacggttgcc aggcccgcgt ccacaacccc gacgtgctgt ggcagatcgt
     6301 cagccagtcg tgcgtgccgt cggccgaggc cggcaagggg ccgggcaagt gcgccaaggt
     6361 cagcccggac ggctacgcca tcctcaagga catcaatggc aaggggcagc atctgctgat
     6421 accaaccacg cgcgtgacag gcgtcgaaga cccgttattg ctgcggtccg gttcgcccga
     6481 ctacttccgc tatgcctggg aagaccggga cttcgtcagc aaggccctgg gcgcgcgagt
     6541 gccggacgaa ctgatgagcc tggcgctgaa ctcggccgac gggcgcacgc agaatcaatt
     6601 ccacatccat gtcgactgcc tgtcgcaaga catgcgcaat gccttgtcgg cgtatgacgg
     6661 cgcaacaacc ggcgcgtggc tcaatacgac gttcaacggc cacccctatc ggctcgagcg
     6721 cgtcgctgcc gcgacggctg ccggcgtgga tccgttcggc ctggtgctca ggcaggccgc
     6781 cgccgcgcaa caagccatgc gcggccacac catcttcatg gcaagcgcgc caggcgggtt
     6841 cttcgtggtg gatgggtact accagcccta cggcccggac gccaatccgg gatcgtcgga
     6901 agagctgcag gaccatagct gcaaggacgt gcggaaatag ggctaccgcc cccgccagtc
     6961 gtcgatcagc cgccggtctt tcttggtggg ccggccttcg cggatttcct gggccggttc
     7021 cggcgccagc ctgcgcagcg cggcggcgcg ctcgcgcgcc ttgatgctct ccggcgtttc
     7081 ttcgtacagc gctcgcgcca ccggcgccgg cccgcgcatg ccgctcacgc cgcgcacttc
     7141 cacatggaac accaggtctt ccttgcggat gctgacgagg tcgcccggcc ccacctcgcg
     7201 cgagggcttg ccgaccacgc cgttcacatg cacccggccc ttgccgattt cgtccacggc
     7261 gatgctgcgc gtcttgtaga agcgcgcggc ccacagccat ttgtcgaggc ggatcttttc
     7321 cattgcgttc catatttgat gtggcgcatc gacgataacc caacctaggt aaaacccgag
     7381 cccggccatt ttgcaattgc ctttcgccat ggcggctctt accattcgga tgtgcatacg
     7441 tagtcacatc gagaccgcag caaggaaaca cgaacaccat ggcaaagatt ctgcccgtcg
     7501 caaggacgct ggcgccggaa ggcatctccc aggaagaatg ggaaatccgc tgcgacatgg
     7561 cggcgatgtt ccgcgccatg gcgcgcctgg gcatgaacga gcagatcgcc aaccactgca
     7621 gcatgatgct gccgggcagc gacaacctgt ttctcatcaa cccgcgcggc taccactaca
     7681 gcgaactgac ggccggcctg ctcatcgtct gcgacctcga cggcaatgtc gtgcgcggcg
     7741 agggcgagct gcgcaaggtg gccttccaca tccatgcccg gctgcacatc aggcatcccc
     7801 aggcccgctg catcctgcac gtgcacccgc aatacctcac cgccctgtcg atgctggaaa
     7861 aaggccgcat ggagctggtg caccagaaca gcgccgcgct ctacgaccgc gtggcctacg
     7921 acgaagtgca caacggcgtg gtgctatggg acgaagaagg cgaccgcatc gccgacgtgc
     7981 tgggcgaaag gaccatcctt gtcatgaaga accacggcgt cacggtcgtg ggcccctcgg
     8041 tggccgaggc cttcgacgag ctctacatcg ccgaacgcac ctgcatgtat cagatgacgg
     8101 cgcagaacac cggcctggcg atgcagacca tcgccgacga cctgcgcgac cgctacaacg
     8161 gcccctgggg cgagcgcttc gacgcccgcc tgcacctcga tgcatggcgc cgcatcttca
     8221 accgggaaga accggaatac gccgcctgaa gcggcgaccg acagacgaac caaggggttt
     8281 gaaaacatgc agatgaagcg aattttcctt tcggccagcc tggcggcggc ggtctgcgcc
     8341 accgccttcg gcccggcgac ggcatgggcc cagaaacgcg gcggcgacgt ggtcgtggcg
     8401 cagcaggccc agccgcccag cctggacgcc cagatcacct tcgcccaggc ctcgcgcaac
     8461 atcaccctgc acatctacga agccctgttc gcccgcaacg acaagggcga gaccatcccc
     8521 gacctggccg agagctatgc caccgcgccg gacggcctgt cgtatgaaat caagctgcgc
     8581 cacggcgtca agttccacaa cctgaaggaa atgaccgcca ccgacgtcaa ggcctcgctc
     8641 gaacgctatg ccaagtacgg cgccagcgcc cagaacatgg ccctggtcga ccacatcgac
     8701 atcgtcgacc cctacactct caagatcgcc atgaagtccc ccttccaggg cttcatcgag
     8761 ttcctgggct cgccgcgcgc gccggtcgcc atctacccgg ccgaagaggc cgccaagggc
     8821 ccgaaccaga tcgacatcat cggcaccggc ccctaccagc tggtcgagta caagcccgac
     8881 agctacgtca agctcaagcg cttcgaagcc tattcggcca atatgaacta ccccggccgc
     8941 gacggcctgg gcgggcgcaa gaccgcctat ctcgacaccg tcaccttccg cttcatgccg
     9001 gaagccggcg cgcgcgaatc cgcgctgcag accggcgaag tgcaggtgct ggaagccatc
     9061 tcggcgacgg ccgccaagcg cctgaagaac gaccccaaca tcaagaccta cgaaatgatg
     9121 ccctgggcgt tccagaccat cttcctgaac gccggcgcgc cgcccaccga caacctcaag
     9181 gtgcgccagg ccatccaggc cgcgctcgac gacgaggaga tcatggccat ctccaccgac
     9241 ggcctgtatc gcatgacgca cggctggcag catccgggca cgccctatta cgcgggcgac
     9301 gtcggcaaga acctgtacaa ccagcacaac ctcgaccgcg ccaggcagct gctgaaagaa
     9361 gccggctaca agggcgagga gctgaccttc atcgccgaca acggtttcaa gaaccacaac
     9421 gacaccgccg tcatcgccac gcagcagctg caggccatcg gcatgaacgt caagctgaag
     9481 atcgccgact ggcccaccac cctggccgcg cgcgagaaac ccaccggctg gaacctcttc
     9541 cccatcatgg tgggcatcga gccgtatgaa ggccccaacg gcgtggcggt gttcttcacc
     9601 ggcgacaaga actggcagaa ccacaaggac gccggcatgg aagcggccat gctcaagctg
     9661 cagaccgcgc cggccctgag cgaccgccag gccgccttcg cgacgatcca gcaaatcatc
     9721 tacgatcagg tctacgccat caaggtgggc gacatcggcg tgctcgaggc cgcccgcgcc
     9781 aacctgaaga acttcgtgcc gcatcgcatc ccgcgcatgt gggacgtctg gtacgaataa
     9841 gccaggatag agacggctgg atttccgcat gctcaggtat ttgatcggcc gcctgctcgg
     9901 ggccgtcccg gttcttttgc tggtgtcgct gctgacgctc agcctgctct ggctggtacc
     9961 gggcgacgcg gcggcggaac tggccggccc gggcgcctcc accgaggaat tgcagcggct
    10021 gcgcaccgaa ctcggcctgg acaagccggt ctacatccag gccgcgcgct ggtacggcaa
    10081 cgtgctgcat ggcgacctgg gccgctcgct gctgctcaac cgcggcgtca ccgaagccat
    10141 cctcgaacgc ctgcccgtca cgggttcgct gaccctgctg gcgctgttca tgacggtcgc
    10201 catcggcatg gccagcggcg tgctggcggc cgtgcggcgc aacacctggg tggaccagct
    10261 atcgatgtcg ctggccctgc tcggcctgtc gctgccggac ttctggctgg gcctggtggg
    10321 catctacctg ttttccgtca cgctgggctg gttcccgacg ggcggctacg tgccgttctc
    10381 ggacagcccc atcggctggc tgcacagcct gttcctgccc gccgcggcgc tggccgccac
    10441 gcagctcggc ctgctggcgc gcatgacgcg ctccagcatg ctcgaagtgc tggcccagga
    10501 ctacatccgc acggcgaagt ccaagggcct gccgcccagg ctggtggtgg gcaagcacgc
    10561 cctggccaat atcgcgattc ccacgctgac cgtgatcggc gtcaatgccg gcatcctgat
    10621 cggcggcgcg gtcatcgtcg aatcggtctt ctccatcccc ggggtgggcc gcctggtcat
    10681 cggcgccatc cagcggcgcg acctgcccgt catccagggc ggcctgctgc tcctggcctg
    10741 cgtgatggtg ctgatcaacc tgctggtcga cattctgtac gccgtattcg atcccagggt
    10801 gcgccatggc cggtaatgtc cttgcctccg acggccgcgc cggtggctgg cccctggcgg
    10861 cccggctgtc gcggcaccgc ctcttcatga cgggcctgtg cctgctgctg gccatcgtcc
    10921 tgctgtcctt gctggccggc tggctggcgc cctactcgcc ggtcgccaac gactaccgct
    10981 accgcctcgg cgcgccgaac gccgcgcact ggatgggcac cgacagcttc ggccgcgacg
    11041 tcttcagccg catgctctac ggcagccggg tgtcgctgcg catcggcctg ttcgtcatgc
    11101 tgtgcaccgg catcatcggc acggccatcg gcctgtgcgc cggctatttc cgccgcctgg
    11161 aaggccccat catgcgcgtg atggacgcgc tcatggcctt cccgggcatc ctgctggcca
    11221 tcgcgctggc ctccgtgctc gggccgtccg aagcgaatgt ggtgctggcc ctgacggtca
    11281 cctacacgcc gcgcaccgcg cggatcatgc gggccgccgc cctggtcctc agcgagatgg
    11341 aatacgtgca ggcggccaag gccgccggcg ccgggcacct gcgcatcctg gtcaggcaca
    11401 tcgccgccaa cagcatggcg ccgctgatcg tgcagatgac cttcatcttc gccatctcga
    11461 tcctggccga ggcggtgctg agcttcatcg gcgtcggccc cccgccgccc agccccacct
    11521 ggggcaacat catcgcggac ggccgcgatt tcatccccga cgcgccctgg gtctgcctgt
    11581 ttcccggcct ggccgtggcc atggcggtgc tgggcctgaa cctgatcggc gacgggctgc
    11641 gcgacgtgct cgacccgcgc ctgcggaccg acctgcgatg aacggcgtat cgcccccgaa
    11701 ccacgccgcg cccctgctcg acgtgcagaa cctcagcacc gtcgtgcagg ccggcggccg
    11761 gcgcctcacg gtgctcgacg acatcagctt cgacatccgt ccgggcgaag ccctggccct
    11821 ggtcggcgag tccggctcgg gcaagagcat ggccgcgctg tccatcatgc ggctgctcgc
    11881 agcacccgcc cgcgtggcgt ccggccgcgc cctgttcaag ggcgccgacc tgttctcgct
    11941 cagcgagtcg cagatgcgcg gcatccgcgg cgcgtcgatc tcgatgatct tccaggagcc
    12001 catgagctcg ctgaacccgg tcttcaccgt gggcgaccag atcggcgagg tgctgcgcta
    12061 ccacaagggc atcaaccgcc gcgcggcgcg ccgccaggcc atcggcttgc tggacctggt
    12121 gggcatcccc gccgcggccc agcgggtcga caactatccg caccagatgt cgggcggcat
    12181 gcgccagcgc gtgatgatcg cc
//
LOCUS       DASWPO010000096         4733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1807792, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000096 DASWPO010000000
VERSION     DASWPO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807792"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VGC69_05900"
     CDS             complement(<1..842)
                     /locus_tag="VGC69_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY0294851.1"
                     /translation="MTASIPIAAIATAPGRGGIGVVRVSGAGLAPLVMRLFGRALAPR
                     HAHYLPFTAADGEVLDQGIALYFPAPHSYTGEDVLELQGHGGPAVLRRLLARCVEAGR
                     EDGLRLAEPGEFTRRAFLNERMDLAQAEAVADLIDASSEAAARGAMASLSGEFSILVN
                     ALADRIVHLRMLVEATLDFPEEEIDFLEKYQARPALEALAEDLDALIRQAKQGVILRE
                     GLHVVLAGQPNVGKSSLLNALTGAGVLVEDALFATLDPSVRKAKTPGGRPYTLTDTVG
                     FVRHL"
     gene            984..2186
                     /locus_tag="VGC69_05905"
     CDS             984..2186
                     /locus_tag="VGC69_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY0294852.1"
                     /translation="MKTLDEIERAHGELTAIRRDIHAHPELAFEETRTSNLVAQRLRE
                     WGIEVHTGLGKTGVVGVLRGAGSSKKTLGLRADMDALPMPELNRFAHASTFSGRMHGC
                     GHDGHTTMLLGAAQYLARHRELFDGTVNFIFQPAEEGGNAGARAMIEDGLFEKFPCDA
                     IFGIHNMPGLPTNKFCFREGPFMASSNRWDIVIRGVGGHAAMPNRGVDPIVISAEVVQ
                     ALQTIISRRRDPLDPAVLSVTQIHAGDAYNVIPNEVVLRGTVRTFTTETLDQIESDMR
                     RIVTTLPEVYGGSGELQFVRAYPPVVNHAKETAFAIQVARDAFGAGQVEENSAQLMGA
                     EDFSFYLQKVPGAYLFLGNGEGGHREAGYHGMGPCQLHNSNYDFNDALLPVGATYWVK
                     LVESFLRK"
     gene            2362..3261
                     /locus_tag="VGC69_05910"
     CDS             2362..3261
                     /locus_tag="VGC69_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0294853.1"
                     /translation="MSVSAVLDRPAASGADQSFEVRPFAGGTGAEIVGLDLNRGLTGA
                     DFARIHRAHLDHHVVVFRDQRITPQQQIDFSRRFGKLMIHVLHQFHLAGHPEILIISN
                     IVENGQAVGLGDAGKEWHSDISYKPLPSLGSLLHAQELPAEGGDTLFANMHLAYDTLP
                     AHLQRAVEGRQAVHSYLARYTRLQSQDGWRPALSAEQIAQVQEVEHPVVRTHPETGRK
                     ALFVSEGFTTHIAGLPEDESRQVLGEIFAHSVKPEFVYRHRWQPHDLLFWDNRSLIHL
                     AGGTPDHLRRKLYRTTIEGDAPF"
     gene            3302..4342
                     /locus_tag="VGC69_05915"
     CDS             3302..4342
                     /locus_tag="VGC69_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294854.1"
                     /translation="MQTATFRERLNEYATAALLAVFIAVVALAAPTPARAEGRIRIAE
                     QYGIVYLLLNVARDQQLIEKQGKAAGLDIQVEWTQLSGGSAINDAVLSGAVDIGGAGV
                     GPLLTLWDKSYGRQNVRGVASLGNLPYYLVSNNPKVKTIADFTDQDRIALPAVSVSVQ
                     SRVLQMAAAKLWGPKQFDKLDPITVALPHPDAAAAIISGGTEITGHFGNPPFQEQELA
                     GNPNAHIVLNSYDVLGGPSSATVLFATEKFRAQNPKTYGAFQAALAEAAAFITAHPDQ
                     AADIYIRVNKAKIDRGFLVKLISNPQVQFKLAPQNTFALAEFMNQVGAIKHKPQSWKD
                     YFFDDAATAQGS"
     gene            4353..>4733
                     /locus_tag="VGC69_05920"
     CDS             4353..>4733
                     /locus_tag="VGC69_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294855.1"
                     /translation="MTAVHAHALPQPLLRASGVTIEYKTPDRLVRATHQVSFDVLPED
                     RFILLGPSGCGKSTLLKAIAGFVAPTEGEFLLDGLSIRGPGPDRVVVFQEFDQLPPWK
                     TVRQNVAFPLRTARRLSRAEADERA"
BASE COUNT          819 a         1612 c         1552 g          750 t
ORIGIN      
        1 aggtgccgga cgaagccgac ggtgtcggtc agcgtgtacg gccggccgcc gggggtcttg
       61 gccttgcgca ccgacgggtc cagggtggcg aacagcgcgt cctccaccag gacgccggcc
      121 ccggtcaggg cgttgagcag gctggacttg cccacattgg gctggccggc cagcaccacg
      181 tgcaggcctt cgcgcaggat gacgccctgt ttggcctgcc tgatgagcgc gtcgagatcc
      241 tccgccaggg cctcgagcgc ggggcgcgcc tggtacttct cgaggaagtc gatttcttct
      301 tcgggaaaat cgagcgtggc ctcgaccagc atgcgcaggt gcacgatgcg gtcggccagc
      361 gcgttgacca ggatggagaa ctcgccggac agcgaggcca tggcgccgcg cgcggcggct
      421 tccgaggaag catcgatgag gtcggccacg gcctcggcct gggccaggtc catgcgctcg
      481 ttcaagaatg cgcggcgcgt gaattcgccg ggttcggcca ggcgcaggcc gtcctcgcgg
      541 ccggcctcca cacagcgcgc caggaggcgc cgcagcacgg ccgggccgcc gtggccctgc
      601 agctcgagca cgtcctcgcc ggtataggaa tgcggcgccg ggaagtacag cgcgatgccc
      661 tggtccagca cctcgccgtc ggcggcggta aacggcaggt aatgggcatg ccgcggggcg
      721 agcgcgcggc cgaagaggcg catgaccagc ggggcgagcc cggcgcccga gacgcgcacc
      781 acgcctatgc ctccacggcc cggggcggtg gcgatggcgg cgatgggaat ggatgcggtc
      841 atggcgggcg gatggcagac ggatggaaag aatataacgt gcccggctcc gggattgccc
      901 tcggcggaca ggtcccggtc ggttgcccat ccgggagcat ggctatgatg tgaagagaat
      961 ccatccgccc gacaggaacc gccatgaaga ccctagacga aatcgaacgc gcccacggcg
     1021 agctcaccgc catccgccgc gacatccatg cccatcccga actggccttc gaggaaacgc
     1081 gcacgtcgaa cctggtggcg cagcgcttgc gggaatgggg catcgaggtg cataccggcc
     1141 tgggcaagac cggcgtggtg ggcgtgctgc gcggcgccgg gtcgtcgaaa aagaccctgg
     1201 gcctgcgcgc cgacatggac gccctgccca tgcccgagct caaccgtttt gcgcatgcat
     1261 cgacgttcag cggccgcatg catggctgcg ggcatgacgg ccacaccacc atgctgctgg
     1321 gggcggcgca gtacctggcc aggcatcgcg agctgttcga cggcacggtg aacttcatct
     1381 tccagcccgc cgaagaaggc ggcaatgccg gcgcacgggc catgatcgaa gatggcctgt
     1441 tcgagaaatt cccttgcgat gccattttcg gcatccacaa catgccgggc ttgccgacca
     1501 acaagttctg ttttcgcgag gggcctttca tggcgtccag caatcgttgg gacatcgtca
     1561 tccgcggcgt gggcgggcat gccgccatgc cgaaccgcgg cgtcgatccc atcgtgatct
     1621 cggccgaggt ggtgcaggcc ttgcagacga tcatttcgcg gcgccgcgat ccgctcgatc
     1681 cggcggtgct gtcggtcacg cagatccacg cgggcgatgc ctacaacgtc attcccaacg
     1741 aagtcgtgct gcgcggcacg gtgcggacct tcacgaccga aacgctggac cagatcgaat
     1801 cggacatgcg ccgcatcgtg accacgctgc ccgaggtcta tggcgggtcg ggcgaactgc
     1861 agttcgtgcg cgcttatccg cccgtggtca accacgccaa ggaaaccgcc ttcgccatcc
     1921 aggtggcgcg cgatgccttc ggcgccgggc aggtcgaaga gaactcggcc caattgatgg
     1981 gcgccgaaga tttctccttc tacctgcaga aggtgccggg cgcgtatctg ttcctgggca
     2041 atggcgaagg cggccaccgc gaggcgggct atcacggcat gggcccctgc cagctgcaca
     2101 attccaacta cgacttcaac gatgccttgc tgccggtggg cgcgacgtat tgggtgaagc
     2161 tggtcgaatc cttcttgcgg aaataagcat atgccgcaac gcgggggttg cggcgccata
     2221 tcactaaaaa ctctgatcta aggcggattt attcgtttga gtaggctgca tgcctgcgca
     2281 taatcgaacg ctcccagtcg gcgccttatg ccggctatgc ataaagcccg ggccgaaccg
     2341 ggccttgtct ggagcaatac catgtccgta tccgccgtcc tcgaccgccc cgccgcgtcg
     2401 ggtgccgacc agagtttcga agtccgtccg tttgccggcg gcaccggcgc cgagatcgtg
     2461 ggcctggacc tgaaccgcgg cctcaccggc gcggacttcg cgcgcatcca tcgcgcccac
     2521 ctggaccacc acgtggtggt cttccgcgac cagcgcatca cgccgcagca gcagatagac
     2581 ttcagccgcc gcttcggcaa gctgatgatc cacgtgctgc accagttcca cctggcgggc
     2641 catcccgaaa tcctgatcat ctccaacatc gtcgagaacg gccaggccgt gggcctgggc
     2701 gatgccggca aggaatggca ttccgacatc tcgtacaagc cgctgcccag cctgggttcg
     2761 ctgctgcacg cgcaggaact gcccgccgag ggcggcgaca ccttgttcgc caatatgcac
     2821 ctggcctacg acacgctgcc cgcgcatctg cagcgcgccg tggaaggccg ccaggccgtg
     2881 cactcctacc tggcccgcta cacgcgcctg cagtcgcagg acggctggcg ccccgcgctc
     2941 tcggccgagc agatcgcgca ggtgcaggaa gtggagcacc cggtggtgcg cacgcacccc
     3001 gaaacgggcc gcaaggcgct gttcgtcagc gaaggcttca ccacgcatat cgcgggcctg
     3061 cccgaagacg agagccgcca ggtgctcggc gagatcttcg cgcacagcgt caagccggaa
     3121 ttcgtctatc gccaccggtg gcagccgcac gacctgctgt tctgggacaa ccgctcgctc
     3181 atccacctgg ccggcggcac gccggaccac ctgcgccgca agctctaccg caccaccatc
     3241 gagggcgacg cgccgttctg agccgcgcgt ccggcttttg ttccgaactt ataggttgtc
     3301 catgcaaacc gccactttcc gcgagcgcct gaacgaatac gcgacggccg ccctgcttgc
     3361 cgtcttcatc gccgtcgtcg cgctggccgc ccccacgccg gcccgggcgg agggccgcat
     3421 ccgcatcgcc gagcagtacg gcattgtcta cctgctgctg aacgtggcgc gcgaccagca
     3481 gctgatcgaa aagcagggca aggcggccgg gctggacatc caggtcgagt ggacgcagct
     3541 gtcgggcggg tcggccatca atgacgccgt gctgtccggc gcggtggata tcggcggcgc
     3601 gggcgtggga cccttgctga cgctctggga caagagctac ggccggcaga acgtgcgcgg
     3661 cgtggcgtcc ctgggcaacc ttccctatta cctggtcagc aacaatccca aggtgaagac
     3721 catcgcggac ttcaccgacc aggatcgcat cgccctgccg gccgtcagcg tgtcggtgca
     3781 gtcgcgcgtg ctgcagatgg cggcggccaa gctgtggggc cccaagcagt tcgacaagct
     3841 cgacccgatc accgtcgccc tgccgcaccc ggacgcggcg gcggccatca tctcgggcgg
     3901 cacggaaatc accggacatt tcggcaatcc gcccttccag gaacaggagc tggcgggcaa
     3961 tcccaatgcg cacatcgtgt tgaattccta cgacgtgctg ggcggcccga gttcggcgac
     4021 cgtgctgttc gccacggaga aattccgcgc ccagaatccc aagacctatg gcgcgttcca
     4081 ggccgccctg gccgaggcgg ccgctttcat caccgcgcat ccggaccagg ccgccgatat
     4141 ctacatccgc gtgaacaagg ccaagataga caggggcttc ctggtcaagc tgatcagcaa
     4201 tccgcaagtg cagttcaagc tcgcgccgca gaacaccttc gcgctggccg agttcatgaa
     4261 ccaggtgggc gccatcaagc acaagccgca aagctggaag gattatttct tcgacgatgc
     4321 cgcgacggcg cagggcagct gagggcgccg acatgaccgc ggtccacgca cacgccttgc
     4381 cgcagccgct cttgcgcgcg tccggcgtca ccatcgagta caagacgccc gaccgtctgg
     4441 tgcgcgccac gcaccaggtc agcttcgacg tgctgcccga agaccgcttc atcctgctgg
     4501 gcccttcggg ctgcggcaag tccaccctgc tgaaagcgat cgccggcttc gtcgcgccga
     4561 cggaaggcga gttcctgctc gacggcctgt ccatccgcgg gcccgggccc gatcgcgtgg
     4621 tggtcttcca ggagttcgac caattgccgc cgtggaagac cgtgcggcag aacgtggcct
     4681 ttcccttgcg caccgcgcgc cgcctgagcc gcgccgaggc ggacgagcgc gcc
//
LOCUS       DASWPO010000097        15052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_825537, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000097 DASWPO010000000
VERSION     DASWPO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825537"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGC69_05925"
     CDS             <1..459
                     /locus_tag="VGC69_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0294856.1"
                     /translation="ELDAVISRSTLSKEDALASALAAAYAARSPVLIDAFKGALPETD
                     ANAALTAAALMGMNNTWYPYLEMAGDEQLKTLPAQLRMNAYATHGGVEKKRFELFALV
                     ASIVGKCHFCVQSHFGILKQEGLSTEQLRDAGRIAAVVNAAALALAAQGK"
     gene            complement(476..991)
                     /gene="ispF"
                     /locus_tag="VGC69_05930"
     CDS             complement(476..991)
                     /gene="ispF"
                     /locus_tag="VGC69_05930"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY0294857.1"
                     /translation="MNIPFRVGQGFDVHALVEGRPLILGGVTIPHTHGLQGHSDADAL
                     LHAMTDALLGAACLGDIGQHFPDTDPKYQGADSRVLLRAAMAKVREEGWEVVNVDATV
                     HAQAPKIGPHAPAMVANIMADLWLRPGTVNIKAKTNEGLGFLGRKEGIAVTVVVLLAS
                     SRAMDNSTGGP"
     gene            complement(988..1677)
                     /gene="ispD"
                     /locus_tag="VGC69_05935"
     CDS             complement(988..1677)
                     /gene="ispD"
                     /locus_tag="VGC69_05935"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY0294858.1"
                     /translation="MTASMIALVPAAGVGARSGGGMPKQYRPLNGQPMLRLAVLALLA
                     DSRISQVRVAVSAGDGWVADALRGLPRTVWRACGGPTRAETVAAALADSGAADSDWVL
                     VHDAARPGLPADALARLIDACLPDPVGGLLALPVADTVKLAGLQSNGAAVVARTMDRD
                     GLWLAQTPQMFRAGVLRGALAAAAGGGLAITDEAGAMEAAGHAPQLVRGHARNFKVTW
                     PDDFDLMEKWL"
     gene            1707..5171
                     /gene="mfd"
                     /locus_tag="VGC69_05940"
     CDS             1707..5171
                     /gene="mfd"
                     /locus_tag="VGC69_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY0294859.1"
                     /translation="MTDSSPPSMVAPLPVGDTIATTLAALKPGARYARPRPPGSADGW
                     LLATLAQQARRPLVVFTADPLEAQRLAEEIPLYAPGLRVRQLPDWETLPYDAFSPHQD
                     LISERLRTLNALIRREVDLLTIPVTTALYRLAPPAFLAAYTFSFRQKDKLDEAALRAQ
                     LVLANYTHVTQVTAPGEFCLRGGLIDLFPMGSAVPYRLDLFDNEIESIRAFDVDTQRS
                     LYPVREVQLLPGREFPMDETARTHFRARFREVFEGDPSRAVPYRDIGSGIAFAGVEYY
                     LPLFFDQTATLFDYLAADTITVTLGDIENAIARFAQDTTSRYQFLKSDRERPVLPPAS
                     LFLDQEGLYAHFRDFPRLALGTELDHPDFATAPDVSVSRRADDPVARLRALLGKPGRV
                     LLCADSAGRRETLVQMLAEQGLVPTMQADTIQAFLDTDARFGLAVAPLASGFVVPASE
                     LTLLTENDLYPGHAPARRGKRSQERTSNVQAMVRDLAELREGDPVVHAQHGIGRYHGL
                     VNMDMGEGEMEFLHLEYANGSTLYVPVSQLHVIARYSGADPEAAPLHQLGSGQWDKAR
                     RKAARQVRDTAAELLALYAQRAAREGYAFKLPLSDYETFAEGFGFEETPDQGAAIDAV
                     IADMTSGKPMDRLVCGDVGFGKTEVALRAAFLAVANSRQVALLCPTTLLAEQHAQTFA
                     DRFADWPVRVVELSRFRSAKEVTAAIEGINDGRVDIVIGTHKILSKEVKFNRLGLVII
                     DEEHRFGVRQKEALKALRAEVDVLTLTATPIPRTLGMSLEGIRDFSVIATAPQKRLAI
                     KTFVRREDGSTLREALLRELKRGGQAYFLHNEVETIHNRRARLEELVPEARIGVAHGQ
                     MPERELEQVMKDFYQQRFNVLLCTTIIETGIDVPSANTIIIHRADRFGLAQLHQLRGR
                     VGRSHHQAYAYLLTPGEDAITANAKKRLEAIQAMEELGAGFYLAMHDLEIRGTGEILG
                     DSQSGNIHEVGFSMYNEMLNEAVRALKAGEEPDLDAPFNLACEVNLHAPALLPSDYCA
                     DVHGRLTIYKRLAHAEDEDDIIAIQEELIDRFGKLPEAAQTLLATHRLRLWAQPLGIV
                     KIDASETQALLQFGPKARVDPVRLIELVQKQRHIRFSGQDKLRIEFTAPQVNQRVDAV
                     KGLLRALK"
     gene            5176..6033
                     /gene="serB"
                     /locus_tag="VGC69_05945"
     CDS             5176..6033
                     /gene="serB"
                     /locus_tag="VGC69_05945"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY0294860.1"
                     /translation="MTTHLVIQSPGLADSHAEHLAALARAEGIVRISRTAMRLLDVDA
                     DAQARGEVRDWAESHHIDAAFVPAGRKLSDCRVLAMDMDSTLINIECIDEIAAHAGRG
                     EQVARITEAAMRGEIKDFAESLRRRVALLAGVAAEALERVYQDRLRLNPGAERLLASA
                     QAAGIKTLLVSGGFTFFTERMKQRLALTAAYSNTLEIVDGKLTGRVLGDIVDGDAKAA
                     HLRAFAAQHGATQTGQIIAMGDGANDLKMLALAGYAVAYHAKPLVRRQTPYTINVCGL
                     DAVLNWFEG"
     gene            6307..8718
                     /locus_tag="VGC69_05950"
     CDS             6307..8718
                     /locus_tag="VGC69_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HEY0294861.1"
                     /translation="MSYIKSRKAPPAKPHKYPAAALTVLTSAALGLPVAALAQTAPAS
                     GSSAQQQTASSSDSSAATLPAVTVTGDGYNPYKPTVMSSPKFTQPIVDTTQTVQVITQ
                     QLIKEQGATTLTEAMRNVAGAGTFNVGENGSTNTGDRIYMRGIDTSNSIYIDGIRDTS
                     QAHRDMFNTDSVEVIKGPSGSDYGRSAPSGSINMVTKQPHLGNSFDASISAGTVANTR
                     PTLDWNQQLSDTSAFRLNMMGMKSDVAGRDGAENNRWGIAPSFAFGLGTDTRVFIDYM
                     HTKQTNVPDGGVPTIGLTGYKSPTMPFLNGASKVDSSNFYGSPLDHDYETTDMATIKF
                     EHDFNSRTTVRNTTRWAENKTNYLLDSWTATSLYGPNGLAYNAAGTNLNPADPSTWYI
                     NRLANINDSSNSIWSNQTNLTSKFNTGSFKHDLSAGFDITREKQYNYGNTSPSLPLIN
                     LYNPGTLGPVIANPALAYGRNGIDNVGTTTTTGIYAFDTVELSDRWEINGGLRLDHYT
                     TDYNCSNNPTATATTDCLGASVKGGGARGWNSGLDVTGNLVNWKLGALYRLAPNGNIY
                     VDYASSAQPPGGSNFSLSAGNTAQNPNLAPQKTRTFEVGTKWELFDKRLLATADVFNS
                     QIYNNVSVNPDGTFSVDGQMRVQGLELGLAGMVTDKWAVNAAYTYQHATHGSNISSSS
                     GVAGINFTNADGSNALPYTPANALSLWTTYNLPYNVTIGGGANFVSGLVRGSDGTQGS
                     PTNTGSYWVFNAMAAYQVNKNLDFQLNVYNMFNKSYIASINKSGYRYFPGAARSAVLT
                     ANIHF"
     gene            8843..9535
                     /locus_tag="VGC69_05955"
     CDS             8843..9535
                     /locus_tag="VGC69_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY0294862.1"
                     /translation="MMLHLAEVLTKDEVRHLRQKLDAAQWVDGRTTAGNLSARVKRNQ
                     QLPEHGELTRELGQVVLQALARHPLYFAAALPLHTVPPLFNRYAEAETYGFHVDGAMR
                     KKPDSPSAEPDWLRTDLSATLFLCEPDEYEGGELTVKDTYGEHAAKLPAGDMVLYPAN
                     SLHCVTPVTQGVRTCSFFWIQSMIRDDKRRNMLFELDQTIQSLRARHGESAETLNLAN
                     CYHNLLREWSEL"
     gene            complement(9549..9878)
                     /locus_tag="VGC69_05960"
     CDS             complement(9549..9878)
                     /locus_tag="VGC69_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEY0294863.1"
                     /translation="MTQSAAVWLLIALALVSANLPFLTERVFALLPYRQGGAPAVKPF
                     WLRLVEVLVFYLLVGAVGYAFEGSLGNRFKQDWEFYAITLCLYLVLAYPGFVYRYLYK
                     RVMRGQS"
     gene            complement(9943..11427)
                     /gene="nuoN"
                     /locus_tag="VGC69_05965"
     CDS             complement(9943..11427)
                     /gene="nuoN"
                     /locus_tag="VGC69_05965"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY0294864.1"
                     /translation="MMQSHFDFALALPEILLLVLGLAILLIDVLSKHPERKTTFGMTL
                     VVLAVLFVVTLIQWINGVHGKTFDGLYVTDPLAHLLKLASYIAVAVTLIYGRYYAQVR
                     DMIKGGELYVLTLFALLGQMVMISAGSLLSVYLGLELMSLPLYALIALRRDDATATEA
                     SMKYFVLGSLASGFLLYGMSMIYGATGHLELSEVAAVIQAGHAQQMALVFGVVFVVAG
                     LAFKLGAVPFHMWVPDVYQGSPTAVTLILGAAPKLAAFAITLRLLVDGLHGLAVSWQP
                     MLMILAVLSLAIGNLTAIMQTNFKRMLAYSTISHMGFVLLGLSAGVVEGNPGAAAGAY
                     GASLFYMITYVLTTLGSFGMVLLLSRQGFECEEIEDLKGLNRRSPWRALIVLLLMFSL
                     AGIPPLVGFYAKLAVLQALIQSGHVALSVVAVIFSLIGAFYYLRVVKVMYFEEPAADA
                     TVAPVSCGVRGVLSINGLLIILLGILPGGLMTLCVQAVQASLLH"
     gene            complement(11437..12939)
                     /locus_tag="VGC69_05970"
     CDS             complement(11437..12939)
                     /locus_tag="VGC69_05970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0294865.1"
                     /translation="MMASEMASHNFPWLTLAIFVPIVFGLLVLALGSDNKPGFTRKLS
                     LVGSVISFLVTIPLYTGFDSSTAAMQFVEKASWIPAFNVNYHLGIDGISLWFVLLTAF
                     ITIIVVLAGWEVITNRIAQYMAAFLILSGFMVGVFAALDGMLFYVFFEATLIPMYLIV
                     GVWGGPNRVYAAFKFFLYTLAGSLLTLVAFVYLWHASGGSFDILAWQQTKLGYTPQIL
                     IFIALLAAFAVKVPMWPVHTWLPDAHVEAPTGGSIVLAAIMLKLGAYGFLRFSLPITP
                     DASHSLAGLIITLSLIAVIYIGLVAMVQDDMKKLVAYSSVAHMGFVTLGFFIFNTAGV
                     EGAIVQMISHGFVSGAMFFCIGVLYDRMHTRRIADYGGVINVMPKFVTCFVLFSMANS
                     GLPATSGFVGEFMVIMGAVQHNFWVGLLAATALILGAAYSLWMVKRVAFGDIANDHVR
                     ELKDVNCREFFILGIMAIAVLFMGIYPKPFTDVMHVSVQALLQHVAVSKL"
     gene            complement(12936..14945)
                     /gene="nuoL"
                     /locus_tag="VGC69_05975"
     CDS             complement(12936..14945)
                     /gene="nuoL"
                     /locus_tag="VGC69_05975"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0294866.1"
                     /translation="MSSSPNLYLLIALAPLAGAILAGLFGTGFLGRFVSRRGAHCLTI
                     FFVLVSTIGSAIVLKDVLAGNTYDGVVYTWSMIGKVPLQIGFQIDALSAMMMMVVTFV
                     SLMVHIYTIGYMADDPGYSRFFAYISLFTFSMLMLVMANNMVQLFFGWEAVGLVSYLL
                     IGFWYTRPTAIFANMKAFLINRVGDFGFVLGIGLLFAYVGSMHYADVFAQGSKLAGMT
                     LPGTDWALITVACVCLFIGAMGKSAQVPLHAWLPDSMEGPTPISALIHAATMVTAGIF
                     MVARFSPLFELSDAALSFIIVIGAIGALFLGILGIIQNDIKRVVAYSTLSQLGYMTVA
                     LGASAYSVAIFHLMTHAFFKALLFLGAGSVIIGMHHDQDIRNMGGLWKRMPITWITFL
                     LGTLALVGTPFFSGFYSKEHIIEAAGAAHVWGAGFAHYATLIGVFVTSLYSFRLYFLV
                     FHGKERFAAHDDHHDHGHDDHGHGHGGVPHESPWVVTLPLILLAIPSVLIGAWVADPM
                     LFGNFFHGVITVLPQHEAMAELHHDWHGWVDFGLEAFASLPFWLVVAGFVVAWYCYLI
                     NQRVPAAIQSSLSGVNKILENKYYVDWVNEQIIARGARCLGRGLWQTGDRNLIDGFVV
                     NGSAKLVGWVASVSRRLQSGFIYHYAFAMIIGIMALVTFFVLIPQ"
     gene            complement(14963..>15052)
                     /locus_tag="VGC69_05980"
     CDS             complement(14963..>15052)
                     /locus_tag="VGC69_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEY0294867.1"
                     /translation="ESAIGLAILVLLFRNLSTINVDELDRLKG"
BASE COUNT         3034 a         5281 c         4569 g         2168 t
ORIGIN      
        1 gaactggacg ccgtgatcag ccgctcgacg ctgtccaagg aagacgccct ggcctcggcc
       61 ctggccgccg cctacgcggc ccgcagcccc gtcctgatcg acgcattcaa gggcgcgctg
      121 ccggaaaccg acgccaacgc cgccctgacg gccgccgcgc tcatgggcat gaacaacacc
      181 tggtatccct atctcgagat ggccggcgac gaacagctca agacgctgcc cgcccaactt
      241 cgcatgaacg cctacgccac ccatggcggc gtggagaaga aacgcttcga actgtttgcc
      301 ctggtcgcct ccatcgtcgg caagtgccac ttctgcgtgc agtcccactt cgggattctc
      361 aagcaggaag gcctgagcac cgagcagttg cgcgatgccg gccgcattgc cgccgtcgtc
      421 aacgccgccg ccctggcgct ggccgcccaa ggcaaataag cggcgccatg caggcctagg
      481 gcccgccggt gctgttgtcc atggcgcggc tgctggccag caacaccacg accgtcaccg
      541 caatgccttc cttgcgcccc aggaaaccca ggccttcatt ggtcttggcc ttgatgttga
      601 ccgtgcccgg ccgcagccac aggtcggcca tgatgttggc caccatggcc ggcgcatgcg
      661 ggccgatctt gggcgcctgg gcatgcaccg tggcgtccac gttgaccacc tcccacccct
      721 cctcgcgcac cttggccatg gcggcgcgca acagcacgcg gctgtcggcc ccctggtact
      781 tgggatcggt atcgggaaaa tgctggccga tgtcgcccag gcatgccgcg cccagcagcg
      841 catcggtcat ggcgtggagc agggcatcgg catcggaatg cccctgaagg ccatgcgtgt
      901 gcggaatcgt cacaccgccc aggatgagcg gtcggccctc caccagcgcg tggacgtcga
      961 agccctggcc tactcgaaac gggatgttca tagccatttt tccatcaggt cgaaatcgtc
     1021 gggccacgtc accttgaaat tgcgcgcatg gccgcgcacc agttgcggcg catggccggc
     1081 cgcctccatc gcgccagcct cgtcggtgat cgccagcccg ccccccgccg cggcggccaa
     1141 tgccccgcgc agcacgccgg cgcggaacat ctgcggcgtc tgcgccagcc acaatccgtc
     1201 gcggtccatg gtacgcgcca ccacggcagc cccgttcgac tgcaatcccg ccagctttac
     1261 cgtatccgcc accggcaagg ccagcaagcc gcccaccggg tccggcaggc aggcgtcgat
     1321 caggcgcgcc agggcgtcgg ccggcaagcc gggccgcgcg gcatcgtgca ccagcaccca
     1381 gtccgaatcg gccgcgccgc tgtcggccaa ggcagccgcc accgtctcgg cgcgagtcgg
     1441 cccgccgcag gcccgccaca cggtgcgcgg caaaccgcgc aaggcatctg ccacccaacc
     1501 gtcgcccgcc gacacggcca cccgcacctg ggaaatgcgc gaatcggcca gcaaggccag
     1561 gacggccagg cgcagcatgg gctgcccgtt caacgggcga tactgcttgg gcatgccgcc
     1621 acccgagcgg gcgcccaccc cggcggcagg aacgagagca atcattgagg cagtcataag
     1681 ctcgggattt tataatcatc gccctgatga ctgattcctc ccccccctcc atggtcgcgc
     1741 cgctgcccgt cggcgatacg atcgccacga cgcttgccgc cctgaaaccc ggcgcccgct
     1801 acgccaggcc gcgcccgccg ggctcggccg acggctggct gctggccacc ctggcgcagc
     1861 aggcccggcg gccgctggtg gtatttaccg ccgatccgct cgaagcccag cgcctggccg
     1921 aagagatccc gctctatgcc cccggcctgc gcgtgcgcca gctgcccgac tgggaaaccc
     1981 tgccctacga cgccttctcg ccgcaccagg acctgatctc cgagcgcctg cgcacgctca
     2041 acgcgctgat caggcgcgag gtcgacctgc tcaccatacc ggtgaccacg gcgctgtatc
     2101 gcctggcgcc accggccttc ctggcggcct acaccttttc cttccgccag aaagacaagc
     2161 tcgacgaggc cgcgctgcgc gcccagttgg tgctggccaa ctacacccat gtcacgcagg
     2221 tgaccgcccc cggcgagttc tgcctgcgcg gcgggctgat cgacctcttt cccatgggtt
     2281 cggccgtgcc ctaccggctc gacctgttcg acaacgagat cgaatccatc cgcgccttcg
     2341 acgtcgacac gcagcgcagc ctctacccgg tgcgcgaagt gcaactgctg ccggggcgcg
     2401 aattccccat ggacgagacc gcgcgcaccc atttccgcgc gcgctttcgc gaggtgttcg
     2461 aaggcgaccc gtcgcgcgcc gtgccctatc gcgacatcgg cagcggcatc gccttcgccg
     2521 gcgtcgagta ttacctgccg ctcttcttcg accagacggc cacgctgttc gactacctgg
     2581 cggccgacac catcaccgtc acgctgggcg acatcgaaaa cgccatcgcc cgcttcgcgc
     2641 aggacacgac cagccgctac cagttcctca agagcgaccg cgaacggccc gtgctgccgc
     2701 ccgcctcgct cttcctcgac caggaaggcc tgtacgcgca cttccgcgac ttcccgcggc
     2761 tggcgctggg caccgaactc gatcatccag acttcgctac ggcacccgat gtcagcgtgt
     2821 cgcgccgcgc cgacgacccc gtggccaggc tgcgcgccct gctcggcaag cccggccgcg
     2881 tgctgctgtg cgccgactcg gccggccggc gcgaaacgct ggtccagatg ctggccgaac
     2941 aaggcctggt gccgaccatg caggccgaca ccatccaggc cttcctggat acggacgccc
     3001 gcttcggcct ggccgtggcg ccgctggcca gcggcttcgt cgtcccggcc agcgagctca
     3061 ccctcctcac cgaaaacgac ctctatcccg gccacgcgcc cgcccggcgc ggcaaacgct
     3121 cgcaggaacg caccagcaac gtccaggcca tggtgcgcga cctggccgag ctgcgcgaag
     3181 gcgatccggt cgtccatgcg cagcacggca tcggccgcta ccacggcctg gtcaacatgg
     3241 acatgggcga aggcgagatg gaattcctgc atctcgaata cgccaacggc agcacgctct
     3301 acgtgcccgt ctcgcagctg cacgtcattg cccgctacag cggcgccgac ccggaagccg
     3361 cgccgctgca ccagttgggt tcgggccagt gggacaaggc gcgccgcaag gcggccaggc
     3421 aggtgcggga cacggcggcc gaactgctcg ccctgtacgc ccagcgcgcc gcgcgcgaag
     3481 gctatgcctt caagctgccg ctttccgact acgaaacctt cgccgagggc ttcggcttcg
     3541 aggaaacgcc cgaccagggc gccgccatcg atgccgtgat cgccgacatg acctcgggca
     3601 agcccatgga ccgcctggtg tgcggcgacg tgggcttcgg caagaccgag gtcgcgctgc
     3661 gcgccgcctt cctggccgtg gccaacagca ggcaggtggc cctgctgtgc cccaccaccc
     3721 tgctggccga gcagcacgcg cagaccttcg ccgaccgctt cgccgactgg cccgtgcgcg
     3781 tggtggaact gtcgcgcttt cgctccgcca aggaagtgac cgccgcgatc gagggcatca
     3841 acgacggccg cgtcgacatc gtgatcggca cccacaagat cctgtcgaaa gaggtcaagt
     3901 tcaaccgcct cggcctggtc atcatcgacg aggaacaccg cttcggcgtg cgccagaaag
     3961 aagcgctcaa ggcgctgcgc gccgaggtcg acgtgctgac gctgaccgcc acgccgatac
     4021 cgcgcaccct gggcatgtcg ctggaaggca tacgcgactt ctcggtcatc gccaccgcgc
     4081 cgcaaaagcg cctggccatc aagaccttcg tgcgccgcga agacggcagc accctgcgcg
     4141 aagccctgct gcgcgagctc aagcgcggcg gccaggccta cttcctgcac aacgaggtcg
     4201 agaccatcca caaccgccgc gcgcggctcg aagaactggt gcccgaggcc cgcatcggcg
     4261 tggcccatgg ccagatgccc gagcgcgagc tggaacaggt gatgaaagac ttctaccagc
     4321 agcgcttcaa cgtgctgctg tgcaccacca tcatcgagac cggcatcgac gtgccctcgg
     4381 ccaacaccat catcatccat cgcgccgacc gcttcggcct ggcgcagctg caccagctgc
     4441 gcggccgcgt cggccgctcg caccaccagg cctacgccta cctgctcacg cccggcgagg
     4501 acgccatcac cgccaacgcc aagaagcgcc tggaggccat ccaggccatg gaagagctgg
     4561 gcgcgggctt ctacctggcc atgcacgacc tggagatccg cggcaccggc gagatcctgg
     4621 gcgactcgca gtcgggcaat atccacgaag tgggcttctc catgtacaac gagatgctca
     4681 acgaggccgt gcgcgccctc aaggccggcg aagagcccga cctggacgcg cccttcaacc
     4741 tggcctgcga agtcaacctg cacgcccccg ccctgctgcc ttcggattat tgcgccgacg
     4801 tgcacggccg cctgaccatc tacaagcgcc tggcgcacgc ggaggacgaa gacgacatca
     4861 tcgccatcca ggaagagctg atcgaccgtt tcggcaaact gcccgaggct gcccagacgc
     4921 tgctggccac gcaccgcctg cgcctgtggg cgcagcccct gggcatcgtc aagatcgacg
     4981 ccagcgaaac ccaggccctg ctgcagttcg gccccaaggc tcgcgtggac ccggtgcgcc
     5041 tgatcgagct ggtgcagaaa cagcgccaca tccgcttctc cggccaggac aagctgcgca
     5101 tcgaattcac cgcgccgcag gtgaaccagc gcgtggacgc cgtcaagggc ctgctgagag
     5161 ccttaaagta aagccatgac cacccatctc gtcatccaat cgcccggcct ggccgacagc
     5221 cacgccgaac acctggcggc attggcccgc gccgaaggca tcgtccgcat cagccggacc
     5281 gccatgcgcc tgctcgacgt cgacgccgac gcgcaagccc gcggcgaagt ccgcgactgg
     5341 gccgagtccc atcacatcga cgccgccttc gtgccggccg gccgcaagct gtccgactgc
     5401 cgcgtgctgg ccatggacat ggactccacg ctcatcaaca tcgagtgcat cgacgagatc
     5461 gccgcccatg ccggccgcgg cgagcaggtg gcgcggatca ccgaggccgc catgcgcggc
     5521 gaaatcaagg acttcgccga gagcctgcgc cgccgcgtgg cgctgctggc cggcgtggcg
     5581 gccgaggcgc tggaacgcgt ctaccaggac aggctgcgcc tgaacccggg cgcggaacgc
     5641 ctgctcgcct cggcgcaggc cgccggcatc aagaccctgc tggtctcggg cggcttcacc
     5701 ttcttcaccg agcgcatgaa gcaacgcctg gcgctcaccg ccgcctattc caatacgctg
     5761 gaaatcgtcg acggcaagct caccggccgc gtgctgggcg acatcgtcga cggcgacgcc
     5821 aaggccgccc acctgcgcgc cttcgccgcg caacacggcg caacacaaac cggccagatc
     5881 atcgccatgg gcgacggcgc caacgacctc aagatgctgg ccctggccgg ctatgccgtg
     5941 gcctatcacg ccaagcccct ggttcggcgg cagacgccct acacgatcaa tgtctgcggc
     6001 ctggacgccg tgctgaactg gttcgaaggc taggcccaag gcccgtggcc aggcaatccg
     6061 cccggcgttt cctcccgcaa agtcccactc ttgcgccatg cgcggcgcat ggcccgggca
     6121 tgttcgcaat cccctcaaaa tattcgcatt ttatttgaaa ctaattgcaa catagtccaa
     6181 tcaaattatc ataataggaa ttatttgcat taagattagc gccacaattc gagggacttc
     6241 cagaaaagtt tcattcgatt ccagctctgc tacctatcta ccctacacac tggaacaagg
     6301 ctctacatgt cgtacatcaa aagccgcaag gcgccccctg caaagccgca caagtacccc
     6361 gccgccgccc tcaccgtgct gacgagcgcc gcgctcggtc tacccgtcgc cgccttggcc
     6421 cagaccgccc ccgcgtccgg cagcagcgcc cagcagcaga ccgcaagcag cagcgacagc
     6481 agcgccgcca ccttgccagc cgtcacggtc acgggcgacg ggtacaaccc ctacaagccc
     6541 accgtgatga gctcgcccaa gttcacccag cccatcgtcg acacgaccca gaccgtccag
     6601 gtcatcaccc agcagctcat caaagagcaa ggcgccacca cgctgaccga agccatgcgc
     6661 aacgtcgccg gcgcgggcac cttcaatgtc ggcgaaaacg gcagcaccaa caccggcgac
     6721 cgcatctaca tgcgcggcat cgacacgtcc aacagcatct acatcgacgg catccgtgac
     6781 acgtcccaag cccaccggga catgttcaat accgattcgg tcgaagtcat caagggcccc
     6841 tcgggttcgg actatggccg cagcgcgccc agcggctcga tcaacatggt caccaagcag
     6901 ccccacctgg gcaacagctt cgacgccagc atcagcgcgg gcaccgtcgc caacacgcgc
     6961 cccacgctgg actggaacca gcagctgtcg gacacctccg ccttccgcct gaacatgatg
     7021 ggcatgaaat cggacgtcgc cggccgcgac ggtgcggaaa acaaccgctg gggcatcgcg
     7081 ccgtcgttcg ccttcggcct gggcaccgac acccgcgtct tcatcgacta catgcacacc
     7141 aagcagacca acgtacccga cggcggcgtc cccacgatag gcctgaccgg ctacaaaagc
     7201 cccaccatgc ccttcctgaa cggcgcgtcc aaggtcgact ccagcaattt ctacggctcc
     7261 ccgctggacc acgactacga aaccacggac atggccacga tcaagttcga acacgacttc
     7321 aacagccgca ccaccgtgcg caacaccacg cgctgggccg aaaacaagac gaactacctg
     7381 cttgattcct ggacggcgac ttcgctgtac ggcccgaacg ggctggcgta caacgcggcg
     7441 ggcaccaacc tcaaccccgc cgacccgtcg acgtggtata tcaaccgcct ggccaacatc
     7501 aacgacagca gcaacagcat ctggagcaac cagaccaacc tgacgagcaa gttcaacacc
     7561 ggctcgttca agcacgacct gagcgccggc ttcgatatca cgcgcgaaaa gcaatacaac
     7621 tacggcaaca cctcgccctc gctgccgctc atcaatctct acaacccggg caccctcggc
     7681 cccgtcatcg ccaacccggc gctcgcctac ggccgcaacg gcatcgacaa cgtaggcacg
     7741 accaccacca ccggcatcta tgccttcgac accgtcgaac tgagcgaccg ctgggaaatc
     7801 aacggcggcc tgcggctgga ccactacacg accgactaca actgttcaaa caatcccacc
     7861 gccaccgcca ccaccgactg tcttggcgca agcgtcaagg gcggcggcgc gcgcggctgg
     7921 aacagcggcc tcgacgtgac cgggaacctc gtcaactgga aactcggcgc gctctaccgc
     7981 cttgcgccca acggcaatat ttacgtcgac tacgcttcct cggcccagcc cccgggcggt
     8041 tccaacttct ccctctccgc tggcaacacc gcccagaatc ccaacctcgc tccgcaaaaa
     8101 accaggacgt tcgaagtggg caccaaatgg gagctgttcg acaagaggtt gctggccacg
     8161 gcggatgtgt tcaacagcca gatctacaac aacgtcagcg tgaacccgga cggcaccttc
     8221 agcgtggacg gccagatgcg cgtgcaaggc ctggaactgg gcctggccgg catggtgact
     8281 gacaaatggg cggtcaacgc cgcctacacc taccagcacg ccacgcacgg cagcaacatc
     8341 agcagcagca gcggcgtcgc aggcatcaac ttcaccaacg ccgacggctc gaacgccctg
     8401 ccctacacgc ccgccaatgc cctttcgctc tggacgacgt acaacctgcc ctacaacgtc
     8461 acgatcggcg gcggcgccaa cttcgtcagc ggactggtcc gtggctcgga cggcacgcaa
     8521 ggcagtccca cgaacaccgg ttcgtactgg gtcttcaacg ccatggctgc ctaccaggtc
     8581 aacaagaacc tggacttcca gctcaacgtc tacaacatgt tcaacaagag ctatatcgcc
     8641 tcgatcaaca agagcggcta tcgctacttc ccaggcgcgg cccgttcggc tgtgctgacg
     8701 gccaacattc acttctaacc gcgccgcgcc agaaaatctg gcgcgtgcgc aaacccacgc
     8761 ctacccgcgt acgcgccggt aggcgttgtc gtttatattg caaacacgaa ttccgcaacg
     8821 ccccatccaa gagtccatag tcatgatgct gcatctcgcc gaagtcctca ccaaggacga
     8881 agtccgacat ctgcgccaga aactcgacgc cgcgcaatgg gtggacggcc ggacgaccgc
     8941 gggcaacctg agcgcccgcg tcaagcgcaa ccagcaattg cccgagcacg gcgaactgac
     9001 ccgcgaactc ggccaggtcg tgctccaggc cctggcccgc catccgctgt atttcgccgc
     9061 ggcgctgccg ctgcacaccg tgccgccgct gttcaaccgc tacgccgaag cggaaaccta
     9121 cggttttcac gtcgacggcg ccatgcgcaa gaaacccgac agcccgtccg cggaaccgga
     9181 ctggctgcgc accgacctgt cggccaccct attcctgtgc gaacccgacg aatacgaagg
     9241 cggcgagctt accgtcaagg atacctacgg cgaacatgcc gccaagctgc cggccggcga
     9301 catggtgctc tacccggcca acagcctgca ttgcgtgacg cccgtcacgc agggcgtgcg
     9361 cacctgttcg ttcttctgga tccagagcat gatccgcgac gacaagcgcc gcaacatgct
     9421 cttcgaactg gaccagacca tccagtccct gcgcgcgcgg catggcgaaa gcgccgagac
     9481 gctcaacctg gccaactgct atcacaatct gctgcgggaa tggagcgagc tgtaagggtg
     9541 gcggacgctc aggactggcc gcgcatcacg cgcttgtaga gataccggta cacaaaaccc
     9601 gggtaagcca gcaccaggta caagcacaag gtaatggcat agaactccca gtcctgctta
     9661 aaacggttgc ccagggaacc ctcgaaggca taccccacgg cgcccaccag caggtagaac
     9721 accaggacct cgacgagcct cagccagaaa ggcttgaccg ccggcgcgcc gccctgccgg
     9781 taaggcagca gggcaaagac acgttcagtc agaaaaggca ggttggcact gaccagcgcc
     9841 agtgcgataa gcagccagac cgcagcggat tgcgtcacga atatccctag aaaaaacaat
     9901 ggccattcgc aagaatggcc attgttgcat ccttatgccg ggtcagtgca gcaacgaagc
     9961 ttgcacggcc tgcacgcaca gcgtcatcag gccgccgggc aggatgccca gcaggatgat
    10021 cagcaggccg ttgatcgaca gcacgccgcg cacgccgcag gacaccggcg cgaccgtggc
    10081 gtcggcggcc ggctcttcga agtacatgac cttgacgacg cgcaggtagt agaaggcgcc
    10141 gatcagcgag aaaatcaccg ccaccacgga cagggccacg tggcccgact ggatcagggc
    10201 ctgcagcacg gccagcttgg catagaagcc caccagcggc ggaatgccgg ccagcgagaa
    10261 catcaggagc agcacgatca gggcgcgcca cgggctgcgg cgattcaggc ccttgaggtc
    10321 ttcgatctcc tcgcactcga agccctggcg cgacagcagc agcaccatgc cgaagctgcc
    10381 cagcgtggtc agcacgtagg tgatcatgta gaacagcgag gcgccgtagg cgcccgcggc
    10441 cgcgcccgga ttaccctcca ccacgccggc cgacaggccc agcagcacga aacccatgtg
    10501 cgagatggtc gagtacgcca gcatgcgctt gaagttggtc tgcatgatgg ccgtcaggtt
    10561 gccgatggcc agcgacagca ccgccaggat catcagcatc ggctgccagc tgaccgccag
    10621 gccgtgcagg ccgtcgacca gcaggcgcag cgtgatggcg aaggccgcca gcttgggcgc
    10681 ggcgcccagg atcagcgtga ccgccgtggg cgagccctgg tagacgtcgg gcacccacat
    10741 atggaaaggc acggcgccca gcttgaaagc caggccggcc accacgaaca ccacgccgaa
    10801 gaccagcgcc atctgctgcg cgtggccggc ctggatcacg gcggccactt cggacagctc
    10861 caggtggccg gtggcgccgt agatcatgga catgccgtag agcaggaagc ccgaggccag
    10921 cgaacccagc acgaagtact tcatggaggc ttcggtggcc gtcgcgtcgt cgcggcgcag
    10981 ggcgatcagc gcgtacaggg gcagggacat caattccagg cccaggtaca ccgacagcag
    11041 gctgccggcc gagatcatca ccatctggcc cagcagggca aagagcgtca gcacatacag
    11101 ttcgccgccc ttgatcatgt cgcgcacttg agcgtagtag cggccgtaga tcagcgtgac
    11161 cgccacggcg atgtaggacg ccagcttgag caggtgagcc agcgggtcgg tcacgtacag
    11221 gccgtcgaag gtcttgccgt gcacgccgtt gatccactgg atcagcgtga ccacgaacag
    11281 cacggccagc accaccaggg tcatgccgaa ggtggtcttg cgctcgggat gcttgctgag
    11341 cacgtcgatg agcagaatgg ccaagccgag aaccagcagc aggatctcgg gcagtgccag
    11401 ggcgaagtcg aagtgggatt gcatcattgt ccagtcttac agtttcgaga ccgccacgtg
    11461 ttgcagcagg gcctgcaccg atacatgcat cacgtcggta aagggcttgg gatagatacc
    11521 catgaacagc acggcgatcg ccatgatgcc gagaatgaag aactcgcggc agttgacgtc
    11581 tttgagctcg cgcacatggt cgttggcgat gtcgccgaag gccacgcgct tgaccatcca
    11641 gagcgagtac gcggcgccca ggatcagggc cgtggcggcc agcaggccca cccagaagtt
    11701 gtgctggacc gcgcccatga tgaccatgaa ttcgcccacg aagccgctgg tggccggcag
    11761 gccgctgttg gccatcgaga acagcacgaa gcaggtgacg aacttcggca tcacgttgat
    11821 cacgccgccg tagtcggcga tgcggcgcgt gtgcatgcgg tcgtacagca cgccgataca
    11881 gaagaacatg gcgcccgaca cgaagccgtg cgagatcatc tgcacgatgg cgccttcgac
    11941 gccggccgtg ttgaagatga agaagcccag cgtgacgaag cccatgtgtg ccacggacga
    12001 gtacgccacc agcttcttca tgtcgtcctg caccatcgcc accaggccga tgtagatcac
    12061 ggcgatcagc gacagggtga tgatcaggcc ggccaggcta tgcgaggcgt ccggcgtgat
    12121 gggcagcgag aagcgcagga agccgtaggc gcccagcttc agcatgatgg ccgccagcac
    12181 gatggagccg cccgtgggcg cctcgacgtg ggcatcgggc agccaggtgt gcaccggcca
    12241 catcggcacc ttgacggcga aggccgccag cagcgcgatg aagatcagga tctggggcgt
    12301 gtagcccagc ttggtctgct gccaggcgag gatgtcgaac gagccgcccg aggcatgcca
    12361 caggtagacg aaggccacca gcgtcagcag cgagccggcc agcgtgtaca ggaagaactt
    12421 gaacgccgcg tacacccggt tcgggccgcc ccacacgccc acgatgaggt acatcgggat
    12481 cagcgtggcc tcgaagaaca cgtagaacag catgccgtcg agcgccgcga acacgcccac
    12541 catgaacccc gagaggatca ggaaggcggc catgtactgc gcgatgcggt tggtgatgac
    12601 ctcccagccg gccagcacca cgatgatggt gatgaacgcg gtgagcagca cgaaccacag
    12661 cgagatgccg tcgatgccca ggtggtagtt gacgttgaac gccgggatcc acgaggcctt
    12721 ctcgacgaac tgcatggcag ccgtggagga gtcgaagccg gtgtagaggg ggatcgtcac
    12781 caggaaactg atgacggagc cgaccaggga cagtttgcgc gtgaagcccg gcttgttgtc
    12841 tgagcccagt gccagcacca gcaggccgaa tacgatcggg acgaagatcg caagcgtgag
    12901 ccagggaaag ttatgggatg ccatctcgct tgccatcatt ggggaatcag cacgaagaaa
    12961 gtcaccagcg ccatgatgcc gatgatcatc gcgaacgcgt agtggtagat gaagccggac
    13021 tgcaggcggc gggaaaccga agccacccag cccaccagct tggcgctgcc gttgaccacg
    13081 aagccgtcga tcaggttgcg gtcgccggtc tgccacaggc cgcggcccag gcacctcgcc
    13141 ccgcgcgcga tgatctgctc gttgacccag tcgacgtagt acttgttctc gaggatcttg
    13201 ttcacgcccg acaggctgga ctggatcgcg gccggaacgc gctgattgat caagtagcag
    13261 taccaggcca ccacgaagcc cgccaccacc agccagaagg gcagcgacgc gaaggcttcc
    13321 aggccgaaat cgacccagcc gtgccagtcg tggtgcagct cggccatggc ctcgtgctgg
    13381 ggcagcacgg tgatcacgcc atggaagaaa ttgccgaaca gcatggggtc ggccacccag
    13441 gcgccgatca gcaccgaggg gatggccagc aggatcagcg gcagggtcac cacccagggc
    13501 gactcgtgcg gcacgccgcc gtggccatgg ccgtggtcgt cgtggccgtg gtcgtgatgg
    13561 tcgtcatgcg cggcgaaccg ctccttgccg tggaacacca ggaagtacag gcggaacgag
    13621 tacagcgaag tgacgaacac gccgatcagg gtggcatagt gggcaaagcc cgcgccccac
    13681 acgtgggccg cgcccgccgc ctcgatgatg tgctccttgg agtagaagcc cgagaagaac
    13741 ggggtgccca ccagggccag cgtgcccagc aggaaggtga tccaggtgat gggcatgcgc
    13801 ttccacaggc cgcccatatt gcggatgtcc tggtcgtggt gcatgccgat gatgaccgaa
    13861 cccgcgccca ggaacagcag cgccttgaag aaggcgtggg tcatcaggtg gaagatggcc
    13921 accgagtagg ccgaggcgcc cagcgccacg gtcatgtagc ccagctgcga cagcgtggag
    13981 tacgccacca cgcgcttgat gtcgttctgg atgatgccca ggatgcccag gaacagcgcg
    14041 ccgatggcgc cgatcacgat gatgaacgac agggccgcat ccgacagctc gaacagcggc
    14101 gagaagcgcg ccaccatgaa aatgccggcc gtcaccatgg tggcggcgtg gatcagcgcc
    14161 gagatcggcg tggggccttc catcgaatcg ggcagccagg catgcagcgg cacctgggcc
    14221 gatttgccca tggcgccgat gaagaggcag acgcaggcca cggtgatcag cgcccagtcg
    14281 gtgcccggca gcgtcatgcc cgccagcttg ctgccttggg cgaacacgtc ggcgtagtgc
    14341 atggagccca cgtaggcgaa cagcaggccg atgcccagca cgaaaccgaa gtcgcccacc
    14401 cggttgatca ggaaggcctt catgttggcg aagatcgccg tggggcgggt gtaccagaag
    14461 ccgatcagca ggtacgacac caggcccacc gcttcccagc cgaagaagag ctggaccatg
    14521 ttgttggcca tgaccagcat cagcatcgag aaggtgaaga gcgagatgta ggcgaagaag
    14581 cgcgaatagc ccgggtcgtc ggccatgtag ccgatggtgt agatgtgcac catcagcgag
    14641 acgaaggtga cgaccatcat catcatggcc gacagggcgt cgatctggaa gccgatctgc
    14701 agggggacct tgccgatcat gctccaggtg tagaccacgc cgtcgtaggt attgccggcc
    14761 agcacgtcct tcaagacaat ggccgagccg atggtcgaca ccagcacgaa gaagatggtg
    14821 aggcaatgcg cgccgcggcg gctgacgaaa cggcccagga agccggtacc gaacaggccc
    14881 gccaggatgg cgcccgctag cggcgccagc gcgatcagca aatagagatt gggggagcta
    14941 gacattttct tccgatactc cgtcagccct tcaggcggtc gagttcatcg acgttgatcg
    15001 tgctcaggtt gcggaacaac agcaccagga tggccaggcc gatggccgac tc
//
LOCUS       DASWPO010000098        13163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1808925, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000098 DASWPO010000000
VERSION     DASWPO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808925"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VGC69_05985"
     CDS             complement(<1..131)
                     /locus_tag="VGC69_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0294868.1"
                     /translation="MQSATETHHPASGAPIVRITALHKSYGDHEVIKGVDLDVHRGEV
                     "
     gene            complement(115..768)
                     /locus_tag="VGC69_05990"
     CDS             complement(115..768)
                     /locus_tag="VGC69_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0294869.1"
                     /translation="MVTFSIWDILRNLLLAARWTVALSLIAFVGGGVVGGLLLVARLA
                     GGRITGRFIDGYVQVFQGTPMLIQLFLAYFGLALLGVDVDAWSAASLALTLNTSAFLT
                     DIWRGCVTAIARGQWEAASSLALSFGEQLRYVILPQAMRLAIAPTVGFMVQVIKGTAL
                     ASVIGFIELTRAGTMIANATFRPFLTYGCVALMYFILCFAISLVARRLERKFHAVRH"
     gene            complement(778..1476)
                     /locus_tag="VGC69_05995"
     CDS             complement(778..1476)
                     /locus_tag="VGC69_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0294870.1"
                     /translation="MTFDFMAVLASWPMLLAGLGWTILLTAVATVLGVFLAIVCAWGR
                     VYGSTLVRWIVGIYVELIRNTPFIIQLFFVFFGLPGLGIKLTPAAASIIAIVLNLGAY
                     AAEILRAGIEATPRGQIEAGYSLALTPTQVFTRVVLPPALKHMWPSLVGQVIIVMLGT
                     AVCGQISMDELSYAANLIQTRTYRAFEAYIIVAVIYLALSMSVRRLLTWLGERFFFGR
                     SAVPPAAGAARRRG"
     gene            complement(1483..2277)
                     /locus_tag="VGC69_06000"
     CDS             complement(1483..2277)
                     /locus_tag="VGC69_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0294871.1"
                     /translation="MKQKFLSAAAGLFVSVALLAAAPSQAAGSLDAIKAKKSISIGIP
                     TDFAPYGFMGTDFQPQGVDVETAKLIAEKLGVKADLVPVSTPNRIPYLQTNKVDLVIS
                     ALGKTEERAKVLDFSIAYAPFYQGIFGPKALSIKSFDDLAGKTIAVTRGTIQDDLLQQ
                     VAPPSLKIQRFEDDASTVTAFITQQTQLIATGGAVAAAAIDRNPQAQLEYKLLLKDSP
                     CFVGLRKGDTDLLNAVNGILRAAKADGTLEKISQKWLKRGTGNLPE"
     gene            2578..3426
                     /locus_tag="VGC69_06005"
     CDS             2578..3426
                     /locus_tag="VGC69_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY0294872.1"
                     /translation="MTASDTIEARIQQHYPELSGADRKLADVLMMQDRQMATYSATEL
                     AALAGVSKASMARFIRRLGFEDFNAFRLQARRQVSTDSPLYLMQQDSAQHSLAQHVQA
                     DIARLQALTASVSEADIDRCVALLVRARRVWIAGFRNSHMTAFYAHTLLATARADVRL
                     LNDSASRYAELMADVAAGDLLLAMDFRRSTHRIAQIAAIAAESGADLIVLTDAAESRL
                     AGLASVALRCPNPGSPVFYSYAAAASLVNLLAMRVIGQTREAAHARAARIEHIHQALD
                     DLDPHD"
     gene            3471..4667
                     /locus_tag="VGC69_06010"
     CDS             3471..4667
                     /locus_tag="VGC69_06010"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEY0294873.1"
                     /translation="MPPVIPIDFHANPRHAAAEPYGAARQAILGAAALARAQAAVTQW
                     PGYAPTALRALPALARNAGVAAIHYKDEGGRFGLGSFKALGGAYAVGRQLCRVLSDKL
                     GRDVSMDELPGDAALRAACAAVTVTCATDGNHGRSVAWGARMFGCACVIYVHATVSPG
                     RAAAIEQFGARVVRTAGNYDDAVRQADRDARAQSWFVISDTSYPGYTDVPRDVMQGYQ
                     LMVDEAARQIPQWPTHIFVQAGVGGFAAAVCGYFWERATPRQPAPRFIVVEPDRADCL
                     MRSARAGRITAVAGGLDTLMAGLACGEVSLLAWDILDHGADAFCTVGDESAVALMRLL
                     AQPLEDDPAIVAGESAVAGLAGALGALRHPAAREALGLGPASRLLCFGSEADTDPVLY
                     RQLVGA"
     gene            4670..5920
                     /locus_tag="VGC69_06015"
     CDS             4670..5920
                     /locus_tag="VGC69_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEY0294874.1"
                     /translation="MDAGTHGAAPRINAGRLLDSLHTLGGIGALEGGGCCRLALTDAD
                     RAGRDWTVARMRALGMSVTIDAIGNGVAVHPGFEALPPVMIGSHIDTVRTGGLYDGNY
                     GVMAGLEVVAALRDAGLRTRRPIAVAFFTNEEGARFQPDMMGSLVYVGGLDLAGTLAT
                     RGIDGAAVGEELARIGYAGAAPVGAPEVDSFIELHIEQGPVLDAQGTRIGIVEGVQGI
                     SWTEFTVHGVSNHAGTTPMSMRRDAGVVAARVAVFARELTRRYGGRQIATVGAIEFTP
                     GLINVIPNRATFTADLRNTDEAVLAQAEAELLAFAQHAAEEEGTRIEHRRLARFEPVA
                     FDPMVVDLVAQEAAALGLSTLRLPSGAGHDAQMLARVCPTGMIFVPSVDGLSHNVREH
                     TAPQDLANGAQVLLQVALRLANRP"
     gene            5944..6897
                     /locus_tag="VGC69_06020"
     CDS             5944..6897
                     /locus_tag="VGC69_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEY0294875.1"
                     /translation="MPRYVNVALGQLGPVQRADSRRQVVQRLCNLMREAHAVGAHLIV
                     YPELALTTFFPRWYIEDEAELDAFYETGMPNADTMPLFALSRELGVGFYLGYAELAQE
                     QGRKVRYNTSILVDRAGRIIAKYRKVHLPGHAEHEPWRRFQHLEKRYFTPGRGFDVTP
                     AFGGVIGMALCNDRRWAETYRVMGLQGVEMVLIGYNTPVHNAPAPLHDDLSLFHNELS
                     MQAGAYQNGTWVVGVAKGGVEEGVDSIAGSCIVAPSGEVAARCITKDDEMAIARCDLD
                     FCAVYKRTTFNFDLHRQPHAYGMIADRKGETLAADGSPWRD"
     gene            complement(6898..8532)
                     /locus_tag="VGC69_06025"
     CDS             complement(6898..8532)
                     /locus_tag="VGC69_06025"
                     /inference="COORDINATES: protein motif:HMM:NF025622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HEY0294876.1"
                     /translation="MERSIAEPSTQTSAIPRWNKPLFLAALVLNGIVAYCILHADRLY
                     VDDLGRVHSGYTGWTGDGRPLTTLIMQALNMGTPLTDLTPLPQILAVLLLSWCALLVA
                     RKFEIRDTATAMLAAFPLGASPFFLENLSYKFDVLTMVLALTLSIVAVCETPWRGWRM
                     GWGALCLLGTLCLYQPALNAFLVFSVLEFLYLMWHDALPGTLLRQALSRLAQAVVAMI
                     PYKLIAIVVMHNLYASYHAALLRPGQWSDLLNNVLSYWELIREALSETSAWTLTAALL
                     LGFFLALVFGLRYVARSWRSSGPVGRIGRLVLAVLMPGVLALSSMGPLLLLSEAVIVP
                     RVMVGIGALISACLVLLCVLWREAVRRPCLQRYGLWVLLGLPAYSMLLLTAVYGNAML
                     AQDRYEHRVSASLADDFVNIGQRYAARGVKLEHYVLDGTAGYPPVLDLTMRKYPFLQR
                     LVPIYLTAGWGWVRDLLGQYDVRQTMDTLPGGLGAAISRKCENPPVRITANYQLYVVD
                     STVVVAFDNAEQCTPEQVQQQTAVQRLGIPKGQLPQ"
     gene            complement(8793..9797)
                     /locus_tag="VGC69_06030"
     CDS             complement(8793..9797)
                     /locus_tag="VGC69_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0294877.1"
                     /translation="MDSYLPAAPRESCSNLIHSSYDVASYPVPYRLLVWRERMANLMD
                     IVLPTLDELERPFQARAEYYQVGGLTFIDCTADAVVLERSPARIATDKVRDFIFYVYV
                     EGGADSISVRASGMGGTSTMASILAVDLGQPIRLRRNAGRMLIFCVPKTLVQEVFPNP
                     EFIHGHRLCGCTPLVRMIISQASHLAGHIGGMSAEMAQAAIQASVRMLLLALGRQAKL
                     SGSARALARAIMLGEAQRYIQAHLADAELSPDAVLKATQLPRRSLYRLFEEEGGLAAY
                     IRHLRLRRAAEDLTNFPHMPVTDIAYGLGFRSPSDFTRAFRRIYAMAPQDFRVMSLSL
                     "
     gene            complement(10133..10774)
                     /locus_tag="VGC69_06035"
     CDS             complement(10133..10774)
                     /locus_tag="VGC69_06035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0294878.1"
                     /translation="MAQLRLGDTAPDFEQQSSVGPIRFYDYLGSSWGVLFSHPADFTP
                     VCTTELGYTAKLAGEFAKRNVKVLALSVDGAESHKQWIGDINDTQSTTVNFPILADAD
                     RKVSELYDMIHPNASATATVRSVFIIDPARKVRLTITYPASTGRNFNEILRVIDSLQL
                     TDNYSVATPVNWQDGDDVIIVPSLKDEAVIKEKFPKGYKAVRPYLRLTPQPNK"
     gene            complement(10904..12739)
                     /locus_tag="VGC69_06040"
     CDS             complement(10904..12739)
                     /locus_tag="VGC69_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0294879.1"
                     /translation="MAYTVPLQDIRFTLNELAGLADVLKLPGLEEVSVDLVDAILEEN
                     ARLVEEVVAPLNVQGDRQPPVWQEGQVHVSPAWAQAYAQFGEGGWQGLPHPVQWGGQG
                     LPKLVSAPVHENFQSASLAFTLCPMLTEAVIEALLTVGSEAQRRRYLPKLVSGQWSGT
                     MNLTEPQAGSDLALVASRAIRQDDGSYRLHGQKIFITYGEHDQAENIVHLVLARTPDA
                     PPGVKGISLFIVPKFLVNDDGSLGKRNDVWCAKLEHKLGIHGSPTCELVYGSGKGEVG
                     EGAVAYLVGEERRGLEYMFIMMNAARFAVGQQGVAVAERASQHAQAYARERIQGRAVE
                     GSAGPVAIAHHPDVQRMLLTMQSLTEAARAVSYVAAAAHDMGEHHADAAVRAGNRALY
                     EYLVPIVKGFSTESAVEVASLGIQVHGGMGFMEETGAAQYYRDARILPIYEGTTAIQA
                     NDFVGRKTVRDGGATAHACIAAMRETLKAVDAEAARAEVGERVGLQLLREHFDQAICA
                     YDDAVAYVLQNAAGQVRAVYAGSVPFLMLAGTVHGGWQMARAALACRRRLAGGSTDAY
                     YRRKLATSLFYAAHVLPRSLGLSAAVRSGVTADACGQQWEQANIS"
     gene            complement(13007..>13163)
                     /locus_tag="VGC69_06045"
     CDS             complement(13007..>13163)
                     /locus_tag="VGC69_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY0294880.1"
                     /translation="ISGAIQHLAGMKDSKVIVAINKDAEAPIFGVADYGLVGDLFTVV
                     PELASAL"
BASE COUNT         2311 a         4465 c         4293 g         2094 t
ORIGIN      
        1 acttcgccgc gatgcacgtc gaggtcgacc cccttgatca cctcatggtc gccatacgac
       61 ttgtgcaagg ccgtgatgcg cacgatgggc gcgccggaag ccgggtgatg ggtttcagtg
      121 gcggactgca tggaacttcc tttccaggcg cctggcgacc aggctgatgg caaaacagag
      181 aatgaaatac atcagcgcca cgcatccgta ggtgaggaac ggacggaagg tggcgttggc
      241 gatcatggtc ccggcccggg tgagctcgat gaagccgatc accgaggcca gcgcggtgcc
      301 cttgatgact tgcaccatga agcccaccgt gggcgcgatg gccaggcgca tggcctgcgg
      361 caggatgacg tagcgcagct gctcgccgaa gctcagcgcc agcgaggagg cggcctccca
      421 ctgcccgcgc gcaatggccg tcacgcagcc gcgccagatg tcggtgagaa aagcgctggt
      481 attgagcgtg agcgccagcg aggccgccga ccaggcgtcg acgtcgaccc ccagcagcgc
      541 caggccgaaa taggcgagga acaactggat gagcatcggc gtgccctgga agacctggac
      601 ataaccatcg atgaaacggc cggtgatgcg gccgccggcc aggcgcgcca ccagcagcag
      661 gccgcccacg acgccgccgc ccacgaaggc gatcagcgaa agcgcgacag tccagcgcgc
      721 ggccagcagc aagttgcgca ggatgtccca aatggagaaa gtgaccatgt cttcctccta
      781 gccccggcgc cgggccgcgc cggcggccgg gggcacggcg ctccggccga agaagaagcg
      841 ctcgcccagc caggtcagca ggcggcgcac ggacatggac agcgcgagat agatcaccgc
      901 cacgatgatg taggcttcga aggcgcggta ggtccgcgtc tggatgaggt tggccgcata
      961 gctcagttcg tccatggaga tctggccgca cacggccgtg cccagcatca cgatgatgac
     1021 ctgccccacc agggacggcc acatgtgctt gagcgcgggc ggcagcacca cccgggtgaa
     1081 cacctgggtg ggcgtgagcg ccaggctgta gcccgcttcg atctgcccgc gcggcgtggc
     1141 ctcgatgccg gcccggagga tctcggcggc gtaggccccg aggttgagca cgatggcgat
     1201 gatcgaagcg gcggccggcg tgagcttgat gcccagcccc ggcaagccga agaacacgaa
     1261 gaacagctgg atgatgaagg gcgtgttgcg gatcagctcg acatagatac cgacgatcca
     1321 gcgcacgagg gtcgagccgt agacccgccc ccaggcgcac acgatggcca ggaacacgcc
     1381 cagcaccgtg gccacggccg tcagcagaat ggtccagccc aggccggcga gcagcatggg
     1441 ccatgaggcc aggacggcca tgaaatcaaa cgtcatgggc gcttattccg gcaggttgcc
     1501 cgtgccgcgc ttgagccact tctgcgagat tttctcgagc gtgccgtcgg ccttggcggc
     1561 gcgcaggatg ccgttgacgg cgttgagcag gtcggtgtcg cccttgcgca ggcccacgaa
     1621 gcacggcgaa tccttgagca gcagcttgta ttcgagctgc gcttgcgggt tgcggtcgat
     1681 ggccgcggcc gccacggcgc cgccggtggc gatcagctgg gtctgctggg tgatgaaggc
     1741 ggtcaccgtg gacgcgtcat cctcgaagcg ctggatcttg agcgagggcg gcgcgacctg
     1801 ctgcaggaga tcgtcctgga tggtgccgcg cgtcaccgcg atggtcttgc cggccaggtc
     1861 gtcgaagctc ttgatcgaca gcgccttggg gccaaagatg ccttggtaga acggcgcgta
     1921 ggcgatcgag aagtcgagga ccttggcgcg ctcttcggtc ttgcccagcg ccgagatgac
     1981 gaggtcgacc ttgttggtct gcaggtaggg aatgcggttg ggggtgctca ccggcaccag
     2041 atcggccttc acgcccagct tttcggcgat cagcttggcc gtctcgacgt ccacgccctg
     2101 cggctggaag tcggtgccca tgaaaccgta gggcgcgaaa tcggtgggga tgccgatgga
     2161 aatggacttc ttggccttga tggcgtcgag cgagccggcg gcctgcgacg gcgcggcggc
     2221 caacagggcg acgctgacaa acaggccggc ggcagcgctc aggaactttt gtttcatgat
     2281 tatctgttta aagaagttgg agacggggaa acctgtacgc aatacgcgtg ccagaattct
     2341 ggcacggcgg tcgaaaatgt acgataactc gctgaaactg gcttgaataa agcatgaaag
     2401 agacgaagcc ggcttggaag aggtgcttac cctgataggt gattaactgg gttgcgtttt
     2461 tctgccataa tgaaacagaa gtttcatgga tagctcgaca tggaaccatc ccactcttga
     2521 tgcgtgccgg caccaattcg gggcaggcac gcaccacaat atggcgcctc cctgcccatg
     2581 accgcgtccg acaccatcga agcccgcatc cagcagcact accccgaact ctccggggcc
     2641 gaccgcaaac tggccgacgt cctcatgatg caggaccgcc agatggccac ctattccgcc
     2701 accgagctgg ccgccctggc cggcgtgtcc aaggccagca tggcgcgctt catccgccgg
     2761 ctgggcttcg aggatttcaa cgccttccgc ctgcaggcgc ggcggcaggt gtcgaccgac
     2821 tcgccgctct acctcatgca gcaggacagc gcccagcaca gcctggcgca gcatgtgcag
     2881 gccgacatcg cccggctgca ggcgctgacg gccagcgtca gcgaggccga catcgaccgc
     2941 tgcgtggcgc tgctcgtccg ggcgcggcgc gtctggatcg cgggattccg caatagccac
     3001 atgacggcct tctatgccca cacgctgctg gccactgccc gggccgacgt gcgcctgctc
     3061 aacgactccg cctcgcgcta cgccgaactg atggccgacg tggccgccgg cgacctgctg
     3121 ctggccatgg atttccgccg cagcacgcac cgcatcgcgc agatcgccgc catcgccgcc
     3181 gagtccggcg ccgacctcat cgtgctgacc gatgccgccg aatcccgcct ggccggcctg
     3241 gccagcgtgg cgctgcgctg ccccaatccg ggttcgcccg tgttctattc ctatgcggcc
     3301 gcggccagcc tggtcaacct gctggccatg cgggtgatcg gccagacgcg cgaggccgcg
     3361 cacgcccgcg cggcgcgcat cgaacacatc caccaggcgc tcgacgacct cgacccgcac
     3421 gactgaactt tctcttcctg cccccgactt tcctgttgcc aaccatgcgc atgccgcccg
     3481 tcattcccat cgacttccac gccaaccccc gccatgcggc cgccgaacct tacggcgccg
     3541 cgcggcaagc catcctgggc gccgcggcgc tggcccgggc ccaggccgcc gtcacgcaat
     3601 ggccgggcta tgcgcccact gccttgcgcg ccctgcccgc cctggcccgc aacgcgggcg
     3661 tggccgccat ccactacaag gacgagggcg ggcgcttcgg cctgggcagc ttcaaggcgc
     3721 tgggcggggc ctatgccgtc ggccggcaac tgtgccgcgt gctgtcggac aagctgggcc
     3781 gcgacgtgtc catggacgaa ctgcccggcg acgccgcgct gcgcgccgcc tgcgccgccg
     3841 tcaccgtcac ctgcgccacg gacggcaacc atggccgctc ggtggcctgg ggcgcgcgca
     3901 tgttcggctg cgcctgcgtg atctatgtgc acgccaccgt cagcccgggc cgcgccgcgg
     3961 ccatcgagca attcggcgcg cgcgtggtgc gcaccgccgg caactacgac gatgcggtgc
     4021 gccaggccga ccgcgatgcc cgggcgcaaa gctggttcgt catctccgac acctcttatc
     4081 ccggctacac cgatgtgccg cgcgacgtga tgcagggcta ccagttgatg gtcgacgaag
     4141 cggcccggca gatcccgcaa tggcccacgc acatcttcgt gcaggccggc gtgggcggct
     4201 tcgcggcggc ggtctgcggc tatttctggg aacgcgccac gccgcggcaa ccggcgccgc
     4261 gcttcatcgt ggtcgaaccc gaccgggccg actgcctgat gcgaagcgcc cgcgcgggca
     4321 ggatcaccgc cgtcgccggc ggactcgaca cgctgatggc gggactggcc tgcggcgagg
     4381 tgtcgctcct ggcctgggac atcctcgatc atggcgccga tgccttctgc accgtcggcg
     4441 acgagtcggc cgtggccctg atgcgcctgc tggcccagcc gctggaggat gatccggcca
     4501 tcgtggccgg cgagtccgcg gtggccggcc tggccggcgc cctgggcgcc ctgcggcacc
     4561 ccgccgcgcg cgaagcgctc ggcctgggac cggcgagccg cctgctgtgc ttcggcagcg
     4621 aggccgacac cgaccccgtc ctctatcgcc agctcgtcgg agcctgatca tggatgccgg
     4681 gacacatggc gcggcgccgc gcatcaacgc cggacggctg ctcgatagcc tgcacacgct
     4741 gggcggaatc ggcgccctgg aaggcggcgg ctgctgccgc ctggccttga ccgacgcgga
     4801 ccgcgcgggc cgcgactgga cggtggcccg catgcgcgca ctgggcatgt cggtgaccat
     4861 cgacgccatc ggcaacggcg tggccgtcca cccgggcttc gaagccctgc cccccgtgat
     4921 gatcggctcg cacatcgaca cggtgcgcac cggcggcctg tacgacggca actacggcgt
     4981 catggccggc ctggaagtcg tggccgccct gcgcgacgcc ggcctgcgca cgcggcgccc
     5041 catcgccgtc gccttcttca ccaacgagga aggcgcgcgt ttccagcccg acatgatggg
     5101 cagcctggtc tacgtcggcg gcctggacct ggccgggacg ctggccacgc gcggcatcga
     5161 cggcgccgcc gtgggcgagg agctggcccg catcggctac gccggcgcgg cgccggtggg
     5221 cgcccccgaa gtcgacagct tcatcgagct gcatatcgag caggggccgg tgctcgacgc
     5281 gcaaggcacg cggatcggca tcgtcgaggg cgtgcaaggc atctcgtgga cggaattcac
     5341 ggtacacggc gtgtccaacc acgccggcac cacgcccatg tccatgcgcc gcgacgccgg
     5401 cgtcgtggcc gcgcgcgtgg ccgtgtttgc gcgcgagctg acgcggcgct acggcgggcg
     5461 gcagatcgcc acggtgggcg cgatcgaatt cacgcccggc ctgatcaacg tcatccccaa
     5521 ccgcgctacg ttcacggcgg acctgcgcaa caccgacgag gccgtcctgg cgcaggccga
     5581 ggccgaactg ctggccttcg cgcagcacgc cgccgaggaa gaaggcacgc gtatcgaaca
     5641 ccgccgcctg gcccgcttcg agcccgttgc cttcgacccc atggtcgtgg acctggtcgc
     5701 gcaggaagcc gccgcgctgg gcctgtcgac cctgcggctg cccagcggcg cggggcacga
     5761 cgcgcaaatg ctggcgcgcg tctgccccac cggcatgatc ttcgtgccca gcgtcgacgg
     5821 cctgtcgcac aacgtgcgcg aacacaccgc gccgcaagac ctcgccaacg gcgcccaggt
     5881 gctgctgcag gtcgcgctgc gcctggccaa ccgcccctga acccaaccac tggagcccca
     5941 tccatgcccc gttacgtcaa tgtcgccctg ggccagctcg gccccgtcca gcgcgccgac
     6001 agccgccgcc aggtggtgca gcgcctgtgc aatctgatgc gcgaagccca cgccgtgggc
     6061 gcgcacctga tcgtctaccc cgaactcgcg ctgaccacgt tcttcccgcg ctggtatatc
     6121 gaggacgaag ccgagctcga cgccttctat gaaaccggca tgcccaatgc cgacacgatg
     6181 ccgctcttcg cgctgtcgcg cgagctgggc gtggggtttt atctgggcta tgcggagctt
     6241 gcgcaggaac aaggccgcaa ggttcgctac aacaccagca tcctggtcga ccgcgcgggc
     6301 aggatcatcg ccaagtaccg caaggtgcac ctgcccggcc acgccgagca cgagccctgg
     6361 cgccgcttcc agcacctgga aaaacgctac ttcaccccgg gccgcggctt cgatgtcacc
     6421 ccggccttcg gcggcgtgat cggcatggcc ttgtgcaacg accgccgctg ggccgagacc
     6481 tatcgcgtca tgggcctgca gggcgtcgaa atggtgctga tcggctacaa cacgccggtg
     6541 cacaacgcgc cggcgccgct gcacgacgac ctctcgctgt tccacaacga gctgtccatg
     6601 caggccgggg cctaccagaa cggcacctgg gtagtgggcg tggccaaggg cggcgtggaa
     6661 gaaggcgtgg acagcatcgc cggcagctgc atcgtcgccc cgtcgggcga agtggcggcg
     6721 cgctgcatca ccaaggacga tgaaatggcc atcgcccgct gcgacctgga tttctgcgcc
     6781 gtctacaagc gcaccacctt caacttcgac ctgcatcgcc agccgcatgc ctacggcatg
     6841 atcgcggacc gcaagggcga gacgctggcg gccgacgggt cgccgtggcg ggattgatta
     6901 ctggggcaat tggcccttgg gtatgcccag gcgctgcacc gccgtctgct gctgcacttg
     6961 ctcgggcgtg cactgctcgg cattgtcgaa ggccaccacc acggtggagt ccaccacata
     7021 cagctggtag ttggccgtga tgcgcaccgg cgggttctcg cacttgcggc tgatcgccgc
     7081 gcccaggccg ccaggcagcg tgtccatggt ctggcgcacg tcgtactggc ccagcaggtc
     7141 gcgcacccag ccccagcccg cggtaaggta gatgggcacc aggcgctgca ggaagggata
     7201 cttccgcatg gtgaggtcga gcaccggcgg gtagccggcc gtgccgtcga ggacgtagtg
     7261 ctcgagcttc acgccgcgcg cggcatagcg ctggccgata ttgacgaaat cgtcggccag
     7321 gctggcgctg acgcggtgtt cgtagcggtc ctgcgccagc atggcattgc cgtagacggc
     7381 cgtcagcagc agcatgctgt aggctggcag gcccagcagc acccacaagc cgtagcgctg
     7441 caggcagggc cggcgcaccg cctcgcgcca cagcacgcac agcagcacca ggcaggccga
     7501 gatcagcgct ccgatgccga ccatcacgcg cggcacgatc acggcctcgc tgagcagcag
     7561 caaggggccc atggaactca gggccagcac gcccggcatg agcacggcca gcaccaggcg
     7621 gccgatgcgc ccgaccgggc cggacgaccg ccagctgcgg gccacgtagc gcaggccgaa
     7681 gaccagcgcc aggaaaaacc ccagcagcag cgccgccgtc agcgtccagg cgctggtttc
     7741 gctcagcgcc tcgcggatca gctcccagta gctcagcaca ttgttgagca ggtccgacca
     7801 ctggccggga cgcagcagcg cggcgtggta gctggcgtag aggttgtgca tcaccacgat
     7861 ggcgatgagc ttgtacggga tcatggccac caccgcctgg gccaggcggc tcagggcctg
     7921 gcgcaagagc gtgccgggca gcgcgtcatg ccacatcaag tacaggaact ccagcacgct
     7981 gaagaccagg aaggcattga gcgccggttg gtacaggcac agcgtgccca ggaggcagag
     8041 cgcgccccac cccatccgcc agccccgcca gggcgtctcg caaaccgcca cgatggacaa
     8101 ggtcagcgcc aacaccatgg tcagcacgtc gaacttgtac gacaggtttt ccaggaagaa
     8161 ggggctcgcg cccagcggga acgcggccag catggccgtg gccgtgtcgc ggatctcgaa
     8221 cttgcgggcc accagcaggg cgcaccagga cagcaggagc acggccagga tctgcggcag
     8281 cggcgtcaga tcggtcagcg gcgtgcccat attgagcgcc tgcatgatca gcgtggtcag
     8341 cggccggccg tcgcccgtcc agccggtgta gccgctgtgg acgcgcccca ggtcgtccac
     8401 atagagacgg tccgcatgca ggatgcagta ggcaacgatg ccgttgagca ccagggcggc
     8461 aaggaacagg ggtttgttcc agcgcgggat cgccgaagtc tgcgtggacg gttccgctat
     8521 cgatctttcc attgatattt gtaggtaaat tttctaatcg cgccaaattg gggatttggc
     8581 atgtcgccac cctggcgagc cgctctctgc tcaaggtgtc gacattggtg gtatgtcttt
     8641 ggttgtattt acaggttttg gttcattcaa aaccttgaaa taattgtgtc aggacatacc
     8701 ctgatggccg ttccgccgtt acattttgcg taccggcatc atatcccgtc cgcccccgcg
     8761 ggggggaaca ggccggcaag cgggcgggcg tctcagaggg acaggctcat gacgcggaaa
     8821 tcctgcggcg ccatggcgta gatgcggcgg aacgcgcgcg tgaagtcgga cgggctgcga
     8881 aagcccagcc cataggcaat gtcggtgacc ggcatatgcg ggaagttggt caggtcctcc
     8941 gccgcgcggc gcaggcgcag gtgccggata taggcggcca ggccgccctc ctcctcgaac
     9001 aagcggtaca ggctgcggcg cggcagctgc gtggccttga gcaccgcgtc gggagacagt
     9061 tcggcatcgg ccaggtgagc ctggatatat cgctgcgcct cccccagcat gatcgcccgg
     9121 gccagcgcgc gcgcgctgcc cgacagcttg gcctgccggc ccagcgcaag cagcagcatc
     9181 cgcaccgagg cctggatggc ggcctgcgcc atctcggcgc tcatgccgcc gatgtgcccg
     9241 gccagatgcg atgcctggct gatgatcatg cgcaccagcg gcgtacagcc gcacagcctg
     9301 tggccgtgga tgaattccgg gttgggaaag acttcctgca cgagcgtttt gggcacgcag
     9361 aaaatcagca tccggccggc gtttcggcgc aggcgtatgg gctggcccag gtcgacggcc
     9421 aggatgctcg ccatcgtgct cgtgccgccc atgcccgagg cacgcaccga gatcgaatcg
     9481 gcgccgccct cgacgtacac gtagaagatg aaatcccgca ccttgtcggt ggcgatgcgg
     9541 gccggcgacc gctcgagcac cacggcgtcg gccgtgcaat cgatgaatgt cagcccgcct
     9601 acctggtaat actccgcgcg ggcctggaaa ggccgctcga gctcgtcgag cgtcggcagc
     9661 acgatgtcca tgagattggc catgcgctcg cgccagacca gcagccggta aggcacggga
     9721 tacgaggcca cgtcgtagct gctgtgaatg agattcgagc agctctcgcg aggcgccgcg
     9781 ggcaggtaac tatccatgag cactaccttt gcaagtcttc gtttatggaa agaatgaacc
     9841 tgctttaaca aaataattcc ggaatccaac gactccccat ggatgaaacc ctagatacta
     9901 aagaatgtaa acttccgttt ctttataaaa atcaaataat tgcgtctgct tctcattaga
     9961 aaagaccctc actgttgaca ctcgggcata aaaaagccgg acatcttcat gtccggccat
    10021 tcgagccgtt cgggaggccc tgtctgtcaa cagtggcttg ccttaaaaca gccttctgct
    10081 tgcctcatct atttaccaag cgctttccct aatttgtatt taagtcccaa cttcatttat
    10141 tgggttgcgg cgtcagacgc agatagggcc gcacggcctt gtagcccttg gggaatttct
    10201 ccttgatcac cgcttcgtcc ttcagcgagg gcacgatgat cacgtcgtcg ccgtcctgcc
    10261 agttcaccgg cgtggccacg ctgtagttgt cggtgagctg cagcgagtcg atcacgcgca
    10321 ggatttcgtt gaaattgcgc cccgtgctgg ccgggtaggt gatggtgagc cgcaccttcc
    10381 tggccggatc gatgatgaat accgagcgca ccgtggccgt ggcgctggca ttggggtgga
    10441 tcatgtcgta gagctcggac accttgcggt ccgcgtcggc caggatgggg aagttcaccg
    10501 tggtcgattg cgtgtcgttg atgtcgccga tccactgctt gtgcgactcg gccccatcca
    10561 ccgacagggc cagcaccttc acattgcgct tggcgaattc gccggccagc ttggcggtat
    10621 agcccagctc ggtggtgcac accggcgtga agtcggccgg atgcgagaac agcacgcccc
    10681 agctgctgcc caggtaatcg tagaaacgga tggggccgac ggaagattgt tgctcgaagt
    10741 caggggcggt gtcgcccaag cgaagttgcg ccatggaaat ctcctgcaaa acggtcggat
    10801 ccggcgcggc ttcgcgagcc ggatgtgtca taggggtctg tgacccgggt aaatcattct
    10861 ggcaccaaac caaaccaggc aaaaatagct tgcctatata accttatgaa atatttgcct
    10921 gttcccattg ctgcccgcag gcgtctgccg tgacgccgct gcgcaccgcc gccgacaggc
    10981 ccagcgagcg cggcaggaca tgcgcggcat agaacaggct ggtcgccagc ttgcggcggt
    11041 agtaggcatc ggtggagccc cccgccaggc gcctgcggca ggccagcgcc gcccgcgcca
    11101 tctgccagcc gccatgcacc gtccccgcca gcatcaggaa gggcacgctg cccgcataca
    11161 ccgcccgcac ctgcccggct gcgttctgca ggacataggc cacggcatcg tcgtaggcgc
    11221 agatggcctg gtcgaaatgc tcgcgcagca attgcaggcc gacgcgctcg cccacctcgg
    11281 cgcgcgcggc ctcggcatcg acggccttga gcgtctcgcg catggcggcg atgcaggcat
    11341 gggccgtcgc gccgccgtcg cgcacggtct tgcggcccac gaagtcgttg gcctggatgg
    11401 ccgtggtgcc ttcgtagatg ggcaggatgc gggcatcgcg gtagtactgc gccgcgccgg
    11461 tctcttccat gaaccccatg ccgccgtgca cctggatgcc caaggaagcc acctccacgg
    11521 ccgactcggt ggaaaagccc ttcacgatgg gcaccaggta ttcgtacagg gcccggttgc
    11581 cggcgcgcac ggccgcatcg gcgtgatgct cgcccatgtc gtgcgccgcc gcggccacgt
    11641 acgacacggc ccgcgccgcc tcggtgagcg actgcatggt gagcagcatg cgctgcacgt
    11701 cgggatggtg cgcgatggcc accgggccgg cagacccttc cacagcacgg ccctggatgc
    11761 gttcgcgcgc ataggcctgg gcatgctggc tggcccgctc ggccacggcc acgccctgct
    11821 gccccacggc aaagcgcgcg gcgttcatca tgatgaacat gtactccagg ccgcgccttt
    11881 cctcgcccac cagataggcc accgcgccct cgcccacctc gcccttgccc gaaccgtaaa
    11941 ccagctcgca cgtggggctg ccgtgtatgc ccagcttgtg ctcgagcttg gcgcaccaga
    12001 cgtcgttgcg cttgcccagg ctaccgtcgt cgttgaccag gaacttgggc acgatgaaaa
    12061 gcgaaatgcc ctttaccccc ggcggcgcgt cgggagtgcg cgccagcacc aggtgcacga
    12121 tgttctcggc ctggtcgtgt tcgccgtagg taatgaagat tttctgcccg tggagccgat
    12181 agctgccatc gtcctggcgg atggcgcgcg aagcgaccag ggccaggtcc gaacccgcct
    12241 ggggctcggt gaggttcatg gtgcccgacc actggccgct gaccagcttg ggcaaatagc
    12301 gccgccgctg cgcctcgctg cccacggtga gcagcgcctc gatcacggcc tcggtcagca
    12361 tcgggcacag cgtgaaagcc aggctggccg attggaagtt ctcatgcacc ggcgccgaca
    12421 ccagcttggg caagccctgc ccgccccatt gcaccggatg cggcaagccc tgccagccgc
    12481 cttcgccaaa ctgcgcatag gcctgcgccc aggccggcga cacatgcacc tggccttcct
    12541 gccacaccgg cggctgccgg tcgccctgca cattcagcgg cgccaccact tcttcgacca
    12601 ggcgcgcatt ctcttcgagt atcgcgtcga ccaggtcgac gctgacctct tccaggcccg
    12661 gcagcttcag cacatcggcc aggccggcca gctcattgag cgtgaagcgg atgtcctgca
    12721 aaggaacggt ataggccaag cgagtctccc tgaacaacga cgatctgcgc cgtcaaccta
    12781 ggcctatctt actcgcgcca aaaacaaaaa gaccacggca agccatggtc tgtcatgcat
    12841 cgagatgcgc atgttgtgag ccgagatcga gacaccgcga gccgaggacc ccgaagaaat
    12901 ccgaagcgca ggcgccagtg gcgccgagca tcggaatttc gcagggcagc ctcggcgcct
    12961 aaagaactca aatcactgcg gaactcaaat cactgcgcaa cccgaatcac agcgccgaag
    13021 ccaactccgg cacgacggtg aacagatcgc ccaccaagcc gtagtcggcc accccgaaga
    13081 tcggcgcttc cgcatccttg ttgatcgcca cgatcacctt cgaatccttc atccccgcca
    13141 aatgctggat ggcccccgag atc
//
LOCUS       DASWPO010000099         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_469150, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000099 DASWPO010000000
VERSION     DASWPO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469150"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VGC69_06050"
     CDS             complement(<1..741)
                     /locus_tag="VGC69_06050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0294881.1"
                     /translation="MNEPIDFLIIGAGIAAASAGYWLAPHGRVVLLEREAHPGYHATG
                     RSAALFMEGYGPAQVRALTLASRAFLESPPEGFTATPLLAPRGALVVAAPGQQDQLTH
                     EWQTLRTVSKTMRLLDAAETLALAPCLRPEQVIGGIYEPDASDMDVHAIHQGYLRGLR
                     KAGGIIACNAEAASLAYRDGLWHVEAGGQTWRAPVVINAAGAWGDAVAQRAGVQPIGL
                     QPCRRSAFTFAPPDGADIRAWPMVIGIDE"
     gene            878..1534
                     /locus_tag="VGC69_06055"
     CDS             878..1534
                     /locus_tag="VGC69_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0294882.1"
                     /translation="MSKAKPHTLDWATLGLRLRAARKQFGWTLSEVAQRSGVSSTTIS
                     RAERGQLALGYENFSALARVLGMGQDMAVMFSEEGSQVGRLHDPVVTRKGEGMTYRGP
                     AFSYHFLATQAAGKQMIPTLSTVHARAVSGPQDYARHDGEEFVYVLNGTIEVYFEDGK
                     TVRLAAGDSLYFDSRIGHAYVCTSRRLARIVGVCTRESTMMATARAGGVDRPVRETAA
                     "
BASE COUNT          262 a          539 c          651 g          255 t
ORIGIN      
        1 ctcgtcgatg ccgatgacca tgggccaggc gcggatgtcg gcgccgtcgg gcggcgcgaa
       61 ggtgaaggcc gatcgccggc agggttgcag gccgatgggt tgcacgccgg cgcgctgggc
      121 gacggcgtcg ccccaggcgc cggcggcatt gatcacgacg ggcgcgcgcc aggtctggcc
      181 gcccgcctcg acgtgccaca ggccgtcgcg gtaggcgagc gaggcggcct cggcgttgca
      241 ggcgatgatt ccgcctgcct tgcgcaggcc gcgcagatag ccctggtgga tggcgtgcac
      301 gtccatgtcg gaggcatcgg gttcgtagat gccgccgatc acctgttcgg ggcgcaggca
      361 gggcgccagg gcgagggtct cggcggcgtc gagcaggcgc atggtcttgg agaccgtgcg
      421 cagcgtctgc cattcgtggg tcagttggtc ttgctgcccc ggcgcggcga cgaccagcgc
      481 accgcgcggc gcgagcaggg gcgttgccgt gaagccttcg ggcggcgatt cgagaaaggc
      541 gcggctggcc agggtgaggg cgcgtacctg cgccgggccg tagccttcca tgaagagcgc
      601 ggcggaacgg ccggtggcgt ggtagccggg atgcgcttcc cgttcgagca gcacgacgcg
      661 gccgtgcggg gccagccagt agccggcgga ggcggcggcg atgcccgcgc cgatgatgag
      721 gaaatcgatg ggttcgttca tactgcggta atctaccgga aattgcgtaa acgcaaattt
      781 gcgttaacgc aaatagtagc gacacggggc tgctgccgag cctagcgttt tccctgaagc
      841 tgctggatct atgacgaacg gaggcgtctc gcgcgtcgtg tcgaaagcga aaccgcatac
      901 cctggactgg gccacactgg gcctgcggct gcgggccgcg cgcaagcagt tcggctggac
      961 gctgtccgag gtggcgcagc gctcgggcgt ctccagcacg accatctcgc gcgccgagcg
     1021 cggccagctg gcgctgggct acgagaattt ttccgcgctg gcccgcgtgc tgggcatggg
     1081 ccaggacatg gccgtcatgt tctcggagga gggcagccag gtcggccggc tgcacgaccc
     1141 ggtcgtcacg cgcaagggcg agggcatgac ctaccgcggc ccggcgttct cctaccattt
     1201 cctggcgacg caggccgcgg gcaagcagat gatcccgacg ctgagcacgg tgcacgcgcg
     1261 cgcggtgagc ggcccgcaag actacgcgcg ccacgacggg gaagagtttg tctatgtgct
     1321 gaacgggacg atcgaggtgt atttcgagga cggcaagacg gtcaggctgg cagccggcga
     1381 ttcgctgtat ttcgacagcc gcatcggcca tgcctatgtc tgcaccagcc gccggctggc
     1441 ccggatcgtg ggggtctgca cccgggagag cacgatgatg gccacggcgc gggccggcgg
     1501 cgtcgatcgg cccgtgcggg aaaccgccgc ctagcggcgc gggcgcgcgt tgccggctag
     1561 gcgcgccagg ccggaaactc catgacggcg acttccaggc cggcggggcc ggcctgcacc
     1621 tcgaaggccg tctcgtcggg gtgcacgaaa aagaccgaaa gcggcggata cgtctcgccg
     1681 cggtattgca tcgcgccgct cagcacc
//
LOCUS       DASWPO010000100         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2968136, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000100 DASWPO010000000
VERSION     DASWPO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968136"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VGC69_06060"
     CDS             <1..816
                     /locus_tag="VGC69_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HEY0294883.1"
                     /translation="ETITELTVDEKITGARARRPNYVSPSFATIAAFNANGAMPHYRA
                     MPESHAVIAGDGLLLIDSGGQYLCGTTDITRVVPVGRPNADQKVDYTLVLKGMIALSQ
                     AAFPRGTPSPMLDAIARAPIWAGGAEYGHGTGHGVGYFLNVHEGPQVISYRAAPLPQT
                     AMEPGMITSNEPGIYRPGRWGVRIENLVACRPWLSGELGEFLGFETLTLCPIDTRCIE
                     RELIRADEIAWLDGYHATVRQRLLPLVSGEARDWLLRRTEPLAGGEQARGLMG"
     gene            928..>2423
                     /locus_tag="VGC69_06065"
     CDS             928..>2423
                     /locus_tag="VGC69_06065"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0294884.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRGLQVTLLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFISTKMRKVNNFTTGQIYESVLRKE
                     RHGDYLGKTVQVIPHITNEIQDFIARGAEASWNGATDVAIVEIGGTVGDIESLPFLEA
                     VRQMSLRLGRNSAAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGIYPNVLLCRADR
                     RVPDDERAKISMFSNVPLDAVISVWDADSIYKIPAMLHAQGVDNIVCDALGLTPPPAD
                     LSMWDGLVDALEHPRDEVTIGMVGKYVDLTESYKSLSEALAHAGIHTHSRVRIEYLDS
                     EEIDERGTDALRGLDAILVPGGFGKRGTEGKIKAIRYARENDVPYLGICLGMQLAVIE
                     YARDVVGLAGASSTEFDADTPHPVVALITEWRDREGQIEKRDASSDLGGTMRKGAQRC
                     PIKPGTLASRIYGAEVNERHRHRYEVNNLYVPKLEAAGMVISSLTPSE"
BASE COUNT          435 a          878 c          749 g          361 t
ORIGIN      
        1 gagaccatca ccgagctcac cgtcgacgag aaaatcaccg gcgcccgcgc caggcggccc
       61 aactatgtca gcccgagctt tgccaccatc gccgccttca acgccaacgg cgccatgccg
      121 cactaccgcg ccatgcccga gtcccacgcc gtcatcgcgg gcgacggcct gctgctgatc
      181 gattcgggcg ggcagtacct gtgcggcacc accgacatca cccgggtcgt gccggtgggc
      241 aggcccaatg ccgaccagaa ggtcgactac accctcgtgc tcaagggcat gatcgcgctg
      301 tcgcaggcgg ctttcccgcg cggcacgccg tcgcccatgc tcgatgccat cgcccgcgcc
      361 ccgatctggg ccggcggcgc cgaatacggc catggcacag ggcatggcgt gggttatttc
      421 ctgaacgtgc acgaagggcc gcaggtgatt tcctaccgcg cggcgccctt gccgcagacg
      481 gccatggagc ccggcatgat cacgtccaac gagcccggca tctaccggcc cggacgctgg
      541 ggcgtgcgca tcgagaacct ggtggcctgc cgcccctggc tgtcgggcga actgggcgaa
      601 ttcctgggct tcgagacgct cactttgtgt ccgatcgata cgcgctgcat cgagcgcgaa
      661 ttgatacgcg ccgacgagat cgcctggctc gacggctatc acgccaccgt gcgccagcgc
      721 ctcctgccgc tggtgtcggg cgaggcgcgc gactggctgc tgcgccgcac cgaaccgctg
      781 gccggcggcg agcaggcgcg cggcctgatg ggttagtcca gggcagtacg ggtaatcctg
      841 acctcgggga aaacccctaa aaaggcgctg gcgcgggtat aatccaaatt tcctgcatgc
      901 acccgatcca cccgggtgcc actcacgatg accaaatacg tatttgtcac cggcggcgtc
      961 gtttcatccc tgggcaaagg gatcgccgct gcgtccctgg ctgccattct cgagtcgcgc
     1021 ggcctgcagg tcaccctgct caaactcgat ccctacatca acgtcgaccc gggcaccatg
     1081 agccccttcc agcacggcga agtcttcgtc accgaagacg gcgccgagac cgacctggac
     1141 ctgggccact acgagcgctt catctcgacc aagatgcgca aggtgaacaa cttcaccacc
     1201 ggccagatct acgaatccgt gctgcgcaag gagcgccacg gcgactacct gggcaagacc
     1261 gtccaggtca tcccgcacat caccaacgag atccaggact tcatcgcccg cggcgccgag
     1321 gcctcctgga acggcgccac cgacgtggcc atcgtcgaga tcggcggcac ggtgggcgac
     1381 atcgaatcgc tgcccttcct ggaagccgtc cgccagatga gcctgcgcct gggccgcaac
     1441 agtgccgcct tcgtgcacct gacgctggtg cccttcatcg cctcggccgg cgaactcaag
     1501 accaagccca cccagcactc ggtgcagaag ctgcgcgaga tcggcatcta tcccaacgtg
     1561 ctgctgtgcc gcgccgaccg ccgcgtgccc gacgacgagc gcgccaagat ctcgatgttc
     1621 tccaacgtgc cgctggacgc cgtgatctcg gtgtgggacg ccgactccat ttacaagatc
     1681 cccgccatgc tgcacgcgca gggcgtggac aacatcgtct gcgatgccct gggcctgacg
     1741 ccgccgcccg ccgacctgtc catgtgggac ggcctggtcg acgcgctcga gcacccgcgc
     1801 gacgaggtca ccatcggcat ggtgggcaag tacgtggacc tcaccgaatc gtacaagtcg
     1861 ctgtccgaag cgctggcgca tgccggcatc cacacgcatt cgcgcgtgcg catcgagtac
     1921 ctcgactccg aggaaatcga cgagcgcggc accgacgcgc tgcgcgggct ggacgccatc
     1981 ctggtgccgg gcggcttcgg caagcgcggc accgaaggca agatcaaggc catccgctac
     2041 gcccgcgaaa acgacgtgcc ctacctgggc atctgcctgg gcatgcagct cgcggtgatc
     2101 gaatacgccc gcgacgtggt cggcctggca ggcgccagca gcaccgagtt cgacgccgac
     2161 acgccgcacc cggtggtggc cctcatcacc gagtggcggg accgcgaagg ccagatcgag
     2221 aagcgcgacg cctcgtccga cctgggcggc accatgcgca agggcgcgca gcgctgcccg
     2281 atcaagccgg gcaccctggc ttcgcgcatc tatggcgccg aggtcaacga gcgccatcgc
     2341 caccgctacg aagtcaacaa cctctacgtg cccaagctcg aagcggccgg catggtgatc
     2401 agctcgctca cgcccagcga gca
//
LOCUS       DASWPO010000101        11341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2790352, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000101 DASWPO010000000
VERSION     DASWPO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790352"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGC69_06070"
     CDS             <1..381
                     /locus_tag="VGC69_06070"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY0294885.1"
                     /translation="HANHSLFWTVMSPTGGGKPEGALAAAIDAELGGLDAFKEAFTKA
                     ALTRFGSGWAWLSVTPQKKLVVESTGNQDSPLMSGNIPILGLDVWEHAYYLKYQNRRP
                     EYIAAFYNVVNWPEVARRYQEALG"
     gene            complement(418..1764)
                     /locus_tag="VGC69_06075"
     CDS             complement(418..1764)
                     /locus_tag="VGC69_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY0294886.1"
                     /translation="MREQSAYCDSSGQEAAASMDPSLGHMHSSVPVPGSASWLRRLLA
                     FVGPGYMVSVGYMDPGNWATDLAGGSRFGYLLLSVVLLSSLMAILLQALAARLGVAAG
                     LDLAQACRAHYPRSVSLLLWGLCEIAIIACDLAEVIGTAIALNLLFGIPLALGAALTA
                     LDVFVVLLLMRRGFRWLEAFVVALLLVIFACFGVQVMLAQPVVAELLAGFIPRTRIMS
                     DPAALYLAIGIIGATVMPHNLYLHSAIVQTRNYPRTREGRREGLRWSVMDSTIALLLA
                     LCVNAAILITSAAVFHAAGRIDVEEIDQAYRLLSPMLGVGLASLAFAIALLASGINST
                     VTATLAAQIVMEGFLSLRMPGWIRRLLTRGLAILPVVVVVGFYGESGAARLLVFSQVV
                     LSMQLPFAVIPLVRLVSDRKVMGEFATGRAVTLLAWSVAGLLVALNIKLLVDMVRG"
     gene            2056..2280
                     /locus_tag="VGC69_06080"
     CDS             2056..2280
                     /locus_tag="VGC69_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0294887.1"
                     /translation="MPHSKKSQNPTSSVIRRARIAADLTQTEAAETVRASLRAWQQWE
                     AGDRAMPPGLFELFMLKTGQWPLDEDEGAK"
     gene            2522..5125
                     /gene="mutS"
                     /locus_tag="VGC69_06085"
     CDS             2522..5125
                     /gene="mutS"
                     /locus_tag="VGC69_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0294888.1"
                     /translation="MMAQYLRLKAEAGPLLLFYRMGDFYEMFYDDAERGARLLSLTLT
                     KRGSSNSAPIPMAGVPVHAMEQYLARLVAMGESVAICEQIGDPAASKGPMERKIVRIV
                     TPGTLTDDALLPAKADRALAAVFLNGKAGRPDPRAGLAWLNLASGEFRVTECAPSQLE
                     SELHRIAPAELVCAEGGVLPFSFEGATTRVPDWHFERDGARANLLAHFKIDSLASFDV
                     EDMPAGICAAGALLRYAARTQSQALAHVQQLQAERPGLYVALDPVTRRNLELTQTLSG
                     EDAPTLFSLLDTCRTPMGSRLLRRWLHHPLRENAPAQARQQAIAALIDARTHADQPFV
                     SGNLLDSLRQGLDAYPDMERIAARLALRSVRPRELASLRDALASLPALRGLLAGLQDS
                     PRLTELTGHLSPDAGIAELLQRAVAAEPAAAIRDGGVIAAGFDAELDELRALATDGGD
                     FLLQLETRERERTGIANLRVEFNRVHGFYIEVSKGQTDKVPEDYRRRQTLKNAERYIT
                     PELKTWEDRVLSAQDRSLAREKWLYENLLDALAPQVPALSACAGALAELDTLAALAEH
                     ARRHDWTAPQLGSEAEIDIEAGRHPVVEKAIERFTPNGCRLDATRRMLLITGPNMGGK
                     STYMRQIALIALLARIGSFVPAARARIGALDRIFTRIGAADDLAGGRSTFMMEMTEAA
                     AILSASTPHSLVLMDEIGRGTSTYDGLSLAWAIAHRLLTYNRALTLFATHYFELTRLP
                     AEQSTAANVHLAAAESAGGIVFLHEVREGPASRSYGIQVAQRAGIPQAVIRQATRELE
                     RLESQGAPTPQLGLFSAAAEAEAQSQIDAEAAESQHAMEALRDELAALDPDSLTPREA
                     LEALYRLKSHL"
     gene            5163..6359
                     /locus_tag="VGC69_06090"
     CDS             5163..6359
                     /locus_tag="VGC69_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0294889.1"
                     /translation="MPISTQIPLQLLGGLSPDEFMRRHWQRKPLLIRQAVPGLKPPIS
                     AAALKKLARSDEVESRLIWREEGLWQMERGPFARLPKVHEPDWTLLVQSMDLHDDAVC
                     ALMQQFRFVPDARLDDAMISIATTGGGVGPHFDSYDVFLLQAAGRRRWRYGMQRDLSL
                     EPDLPLKILSRFQPEEDHVLEPGDMLYLPPQAAHDGIAMDDDCMTISIGFRAPSLATL
                     ARGLLEAAADQAMARLGQPAGPYGEPPLPGPRLDGLYRDPGQPASTHPAELPDAMVDA
                     TLATLARLRFDPALAARFLGCWFTEPNTLSVFTSAHDTPDLEAGWPAQGTLVLDRRTR
                     MLYRGRQLFINGETAPVPPSPALRALADERRLDCRDARCRKLDEDSLACLSDWLSAGW
                     LHYAAQ"
     gene            complement(6430..7611)
                     /gene="bamC"
                     /locus_tag="VGC69_06095"
     CDS             complement(6430..7611)
                     /gene="bamC"
                     /locus_tag="VGC69_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEY0294890.1"
                     /translation="MNKRHAGRWAKPAASMALALSLAQLAGCSSIGKAIGVDNPIDYK
                     SASSSDVEPLNIPPDLTQAASDPRYRAPDAGGATSFSQYQQQSKDAASAVATGKSPSG
                     VLPQRDDMHIERDGDLRWLVIDHPPEQIFPKLVDFWTSVGFTLNTNNPQAGLLETDWA
                     ENRAKIPDSWLHDVLGTIFNEAYDSGERERFRTRVERVGDHTEIFLSHQQMTEKMENS
                     GPSALAVWVPGQEDAGLNAAMLARMMVFLGTDADRARQLLAQAQVSPAKVDVQSVQTA
                     QGASELTIGEAFDRAWRRVGVALDSGGFSVQDRDRNAGDYFVRYLDTDTGVRPEDPNI
                     FSRYLFGVKPAVAPQYRVHLSGSGSSGAQTQVTVLDSNGARDTSPTAQRMLGVLKDKM
                     Q"
     gene            complement(7629..8531)
                     /gene="dapA"
                     /locus_tag="VGC69_06100"
     CDS             complement(7629..8531)
                     /gene="dapA"
                     /locus_tag="VGC69_06100"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY0294891.1"
                     /translation="MTTTTPAAPLFQGSLVALVTPMHPDGSLDYEAYRALIDWHVAEG
                     SNALVVVGTTGESPTVSIDEHAELIRVAVEHAAGRIPVVVGVGANSTEEAIHLSRHAL
                     KVGAQAGLSVVPYYNKPTQEGLYRHFRTIAEAVELPTLLYNVPGRTVADMSNETVLRL
                     AEVPGIIGVKDATGDIGRGALLLREAPAGFQILSGDDPTAAALILLGARGNISVTANV
                     APRLMHDLCTAALAGDVPKVRELNARLARLNKALFVEANPIPVKWALAQMGRTKLGLR
                     LPLCELGAAHHAMVRQALSETGLL"
     gene            complement(8626..9273)
                     /locus_tag="VGC69_06105"
     CDS             complement(8626..9273)
                     /locus_tag="VGC69_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0294892.1"
                     /translation="MNEIIQTIAVYALPLIFAITLHEAAHGYVARLFGDPTAAQAGRI
                     SLNPARHIDPVGTLLVPIVMLLGAKLLGGPSVLFGWARPVPVNFGRLRRPKRDMLWVA
                     LAGPAANLAMALAWALLLRACAAMGLQEHFWFEMALAGVNVNLVLMAMNLLPVPPLDG
                     GRIVFSLLPNRAAWHYARIEPYGLMIVMLLLVTGSLGLFLGPLLALGRLVVGWIL"
     gene            complement(9308..9934)
                     /locus_tag="VGC69_06110"
     CDS             complement(9308..9934)
                     /locus_tag="VGC69_06110"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0294893.1"
                     /translation="MALLLTLHPDNPQPRLLRQAVQWLRDGGLLAVPTDSSYAVVARL
                     DDKAAADAMRRLRGLDERHHLTLLCRDLAEIGMMARVDNRQYRLIKAATPGPWTFILE
                     ATREVPRRVSHPSRKTIGIRVPDHKVALALLEEIGEPLLSTTLIPRGEEEALNDPREI
                     VERYAHELAAVVDAGACPQAPTTVLDLTGAEPVVLRLGRGDPAQLGLA"
     gene            10013..10876
                     /locus_tag="VGC69_06115"
     CDS             10013..10876
                     /locus_tag="VGC69_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY0294894.1"
                     /translation="MRMDDSRESDNIEDRRSAGPRIVGRGTIGIGTIVLALVAMYFGV
                     DPSVVLNLAAGGGQPAPQQQSVPAGKPTGSDPTEHFVARVLGETEDTWSAIFQQQFHR
                     QYSAPTLVLFRGATPTACGTGQAAMGPFYCPGDSKVYLDMGFFDDMSRQLNAPGDFAR
                     AYVIAHEVGHHVQHLLGITDQVDAARQRSPGQANALSVRVELQADCYAGIWAQHANQT
                     HHILENGDIESGLNAANQIGDDTLQKRSQGYVVPDSFTHGSSAQRVRWFKRGFESGDL
                     RQCDTFGASQL"
     gene            complement(10878..11150)
                     /locus_tag="VGC69_06120"
     CDS             complement(10878..11150)
                     /locus_tag="VGC69_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294895.1"
                     /translation="MIQNNFVYKGYRLTAKVLRGTPEEAPPNSSGPIFTATVQIVAAN
                     SVQDPGHEYPVPCFEQGNFVYSPREAVQAAVSHGQSIVDGLMGVQT"
BASE COUNT         2010 a         3979 c         3663 g         1689 t
ORIGIN      
        1 cacgccaatc acagcctgtt ctggaccgtc atgtcgccca cgggcggcgg caagccagag
       61 ggggcgctgg ctgccgccat cgacgccgaa ctgggcggcc tggacgcgtt caaggaggcg
      121 ttcaccaagg cggcgctgac gcgctttggc agcggctggg cctggctgag cgtgacgcca
      181 cagaaaaagc tggtcgtcga gagcaccggc aaccaggaca gccccctgat gtccggcaac
      241 atacccatcc tggggctgga tgtatgggag cacgcttact atctgaagta ccagaaccgg
      301 cgtcccgaat acatcgcggc tttctacaac gtcgtcaact ggcccgaggt cgcgcgccgc
      361 taccaggagg cgcttggttg acgccggctc ggggataggc cgcggcgcgc ccgcagccta
      421 tccccgcacc atatcgacca gcagcttgat gttaagcgcc acgagcaggc ccgccacgct
      481 ccatgcaagc agcgtgactg cgcgccccgt ggcgaactcg cccatcactt tgcgatcgga
      541 cacgagacgc accagcggga tgacggcgaa aggcaactgc atcgacagca ccacctggct
      601 gaacaccagc agcctggccg cgccgctctc gccatagaaa ccgacgacca cgacgacggg
      661 gagaatcgcc aggccgcgcg tcagcaagcg ccggatccag cccggcatgc gaaggctcag
      721 gaatccctcc atgacgatct gtgcggccag ggttgcggtc acggtcgagt tgatgccaga
      781 cgccagcaag gcgattgcga acgccagcga cgccagcccc acgcccagca tcggcgacag
      841 caagcggtag gcctgatcga tttcctcgac gtcgatacgg cctgcggcat gaaaaaccgc
      901 cgccgaggtg atcaggatgg ccgcattgac gcacagcgcc agcagcagcg cgatggtgct
      961 gtccatcacc gaccagcgca atccttcgcg ccgcccctcc cgcgtgcgcg ggtaattgcg
     1021 tgtctgcacg atggcggaat gcaggtatag gttgtgcggc atgaccgtgg ctccgatgat
     1081 gccgatggcg aggtagagcg cggcggggtc ggacatgatc cgcgtccgtg ggataaagcc
     1141 cgccagcaat tccgcgacca ccggctgcgc cagcatgacc tggacgccga agcaggcgaa
     1201 gatgaccagc agcagcgcga cgacgaaggc ctccagccat cgaaacccgc gccgcatcag
     1261 cagcaagacg acgaaaacgt ccagggccgt caacgccgcg ccgagggcca ggggtatgcc
     1321 aaacaacaga ttcaacgcga tcgccgtgcc gatcacctcc gccaggtcgc aggcgatgat
     1381 ggcgatttcg cacaagcccc agagcaagag actcacgctg cggggatagt gggcacggca
     1441 cgcctgggcc aggtcgaggc ccgccgcgac gcccaggcgc gccgcgagcg cctgcagcag
     1501 gatcgccatc aggctggaaa gcaggacaac cgaaagcagc aggtagccga accgcgagcc
     1561 ccccgccaga tccgtcgccc agttgcccgg gtccatatac ccgaccgaca ccatgtagcc
     1621 cgggccgaca aaggccagca gccgacgcag ccagctggcc gatccaggca cgggaacact
     1681 gctgtgcata tgccccaggc tcggatccat ggacgccgcg gcttcctggc cggaggaatc
     1741 gcaataggcg ctctgctctc tcatgccgtc gttcctcgag cccggccaag gccatgcgga
     1801 cgcgcatgcg ttcctggcga tcgggacttt catctaaaga ttaatgattc tcattattaa
     1861 taatgccaca tatttcctct ttgttaacgg gaaatgacat gcaggcgcgc cattgcgaag
     1921 cctcgcctgc ccgcaaggca cacgccccct ctgcaccgtc ggttcccatt tgtgcggcga
     1981 accctggaat acgctcattg agcgtatact gcccgacaat acccgcgcgt gcgcgggctc
     2041 ctgccgcccg cccccatgcc ccactcgaaa aagtcgcaaa atccaacatc cagtgttatt
     2101 cgccgggccc gcatcgctgc ggacctgacc cagacggaag cagcggaaac cgtccgggct
     2161 tcgcttcgcg cgtggcagca atgggaagcg ggtgaccgag ccatgccgcc tggcctgttc
     2221 gagctgttca tgctcaagac cgggcaatgg ccgctcgacg aagacgaagg ggcgaagtaa
     2281 gcatcgaggg ctgagacatg acaggcgcag aagccagaaa gaccggcgac gccggtatcg
     2341 caatacacgg gcatccggtc cgcaagacag attcggagcc tccccctggg agccagagcg
     2401 tatctgggag gggatgcaga acacggcaag atatgtcccg aacttgtcct gaaaattcgc
     2461 cgcgcaaatt aataagttat cgaccttaat ggaaaaaata gacgtttccg gccacacgcc
     2521 catgatggcc cagtacctcc gcctgaaggc ggaagccggg cccttgctgc tcttctatcg
     2581 catgggcgat ttctacgaga tgttctacga cgacgccgaa cgcggcgcgc gcctgctgag
     2641 cctcacgctg accaagcgcg gctcgtccaa cagcgccccc atccccatgg cgggcgtgcc
     2701 ggtgcacgcc atggagcaat acctggcgcg gctggttgcg atgggcgagt ccgtggcgat
     2761 ctgcgaacaa ataggcgacc ccgccgcgtc caagggtccg atggagcgca agatcgtgcg
     2821 catcgtcacg ccgggcaccc tcaccgacga tgccctgctg cccgccaagg ccgatcgcgc
     2881 gctggccgcg gttttcctca atggcaaagc cggcaggccg gatccccgcg ccggcctggc
     2941 ctggctgaac ctggccagcg gcgagttccg cgtcaccgaa tgcgcgccgt cgcagctcga
     3001 atccgagctg caccgcatcg cgccggccga gctcgtctgc gcggaaggcg gcgtcctgcc
     3061 cttttccttc gagggcgcca ccacccgcgt gcccgactgg catttcgagc gcgacggcgc
     3121 ccgcgcgaat ctgctggcgc atttcaagat cgattcgctg gcgagcttcg atgtggaaga
     3181 catgccggcc ggcatctgcg cggccggcgc cctgctgcgc tatgccgcgc gcacgcaatc
     3241 gcaggcgctg gcccacgtgc agcaactgca ggccgaacgg cccggcctgt acgtggcgct
     3301 ggatccggtc acgcgccgca acctcgaact cacccagacg ctgtcgggcg aagacgcgcc
     3361 cacgctgttc tcgctgctcg atacctgccg cactcccatg ggcagccgcc tgctgcgccg
     3421 ctggctgcat cacccgctgc gcgagaacgc gccggcccag gcccggcagc aagccatcgc
     3481 cgcgctcatc gacgcgcgca cccatgccga ccagcccttc gtttcgggca acctgctcga
     3541 cagcctgcgc cagggcctgg acgcctatcc cgacatggag cgcatcgccg cgcgcctggc
     3601 cctgcgctcg gtgcgcccgc gcgagctcgc cagcctgcgc gatgccctgg ccagcctgcc
     3661 ggcgctgcgc ggcctgctgg ccggcctgca ggacagcccg cgcttgaccg aactcaccgg
     3721 ccatttgtcg cccgatgccg gcattgccga actcctgcaa cgcgccgtgg ccgccgaacc
     3781 cgccgcggcc atccgcgacg gcggcgtgat cgccgcgggc ttcgacgccg aactcgatga
     3841 attgcgcgcc ctggccaccg acggcggcga tttcctgctg cagctggaaa cccgcgaacg
     3901 cgaacgcacc ggcatcgcca acctacgcgt cgaattcaac cgcgtgcacg gtttctacat
     3961 cgaggtcagc aaagggcaga ccgacaaggt gcccgaggac taccgccgcc gccagacgct
     4021 gaaaaacgcc gaacgctaca tcacgcccga gctcaagacc tgggaagacc gcgtgctgtc
     4081 ggcgcaggac cgctcgctgg cgcgcgagaa gtggctgtac gagaacctgc tcgacgcgct
     4141 ggccccgcag gtgcccgccc tgtcggcctg tgccggcgcg ctggccgaac tcgacacgct
     4201 ggccgcgctg gccgagcacg cgcgccgcca cgactggacc gcgccgcaat tgggtagcga
     4261 ggctgaaatc gatatcgagg ccggccgcca tcccgtggtc gagaaagcca tcgagcgctt
     4321 cacgcccaat ggctgccggc tcgacgcgac acgccgcatg ctgctcatca ccggcccgaa
     4381 catgggcggc aagtcgacct atatgcggca gatcgcgctg atcgccctgc tggcgcgcat
     4441 cggcagcttc gtgccggccg cgcgcgcgcg gatcggcgcg ctcgaccgca tcttcacccg
     4501 catcggcgcg gccgacgacc tggccggcgg ccggtcgacc ttcatgatgg aaatgaccga
     4561 ggccgccgcc atcctgtcgg ccagcacgcc ccacagcctg gtgctgatgg acgagatcgg
     4621 ccgcggcacg tccacctacg acggcctgtc cctggcctgg gccatcgccc accggctgtt
     4681 gacctacaac cgggccctca cgctgttcgc cacgcactac ttcgagctta cgcgcctgcc
     4741 ggcggagcaa agcacggcgg ccaacgtgca cctggccgcg gccgaatcgg ccggcggcat
     4801 cgtgttcctg cacgaagtgc gcgagggccc ggccagccgc agctacggca tacaggtggc
     4861 gcagcgcgcc ggcatcccgc aggccgtcat ccgccaggcc acgcgcgaac tggaacgcct
     4921 ggaatcgcaa ggcgcgccca cgccgcagct ggggctgttc tccgccgcgg ccgaggccga
     4981 ggcgcaaagc cagatcgacg ccgaggccgc cgaatcgcaa catgccatgg aggccttgcg
     5041 cgacgaactg gccgcgctgg acccggacag cctcacgcca agagaagcgc tcgaagcctt
     5101 ataccggcta aagtcgcatc tgtgagccgc ggcagcccgc ggcggctcga gaacccagac
     5161 tcatgcccat ctccacgcaa attccgctgc aactactagg cggcctaagt ccagacgaat
     5221 tcatgcgccg ccactggcag cgcaagccgc tcttgatccg gcaggccgtc ccgggcttga
     5281 agccgcccat ctcggccgcc gcgctcaaga agctggccag gagcgacgag gtcgagtcgc
     5341 ggctgatctg gcgcgaggaa gggctatggc agatggaacg cgggcccttc gcccgcctgc
     5401 ccaaggtcca cgaacccgac tggaccctgc tggtgcaaag catggacctg cacgacgacg
     5461 ccgtctgcgc gttgatgcag cagttccgct tcgtgcccga tgcccgcctg gacgacgcca
     5521 tgatcagcat cgccaccacg ggcggcggcg tggggccgca tttcgacagc tatgacgtct
     5581 tcctgctgca ggccgcgggc cggcggcgct ggcgctacgg catgcagcgg gacctgtcgc
     5641 tcgagcccga cctgccgctc aagatcctga gccgcttcca gcccgaggaa gaccatgtgc
     5701 tggagcccgg cgacatgctg tacctgcccc cgcaggccgc ccacgacggc atcgcgatgg
     5761 acgacgactg catgacgatc tccatcggct ttcgcgcgcc cagcctggcc acgctggccc
     5821 gcggcctgct cgaggcggcc gccgaccagg ccatggcccg gctgggccag cccgccggcc
     5881 cctacggcga accgcccctg cccggcccgc ggctcgacgg cctgtatcgc gacccgggcc
     5941 agcccgcctc gacgcatccg gccgaactgc ccgacgccat ggtggacgcc acgctggcca
     6001 cgctggccag gctgcgcttc gacccagccc tggccgcgcg cttcctgggc tgctggttca
     6061 ccgaacccaa tacgctgagc gtgttcacct ccgcccacga cacccccgac ctcgaggccg
     6121 ggtggcccgc gcagggcacg ctcgtgctcg accgccgcac ccgtatgctc tatcgcggcc
     6181 gccagttgtt catcaacggc gagaccgccc ccgtgccgcc ctcgcccgcc ctgcgcgcgc
     6241 tggccgacga acgccgcctg gactgccgcg acgcgcgctg ccgcaagctc gacgaggaca
     6301 gcctcgcctg cctgtcggac tggttgtcgg cgggctggct gcattacgcc gcacaataaa
     6361 aaaacgggcc cgaggtcggt cacctcgggc ccgttttgca aacagggcgc aggcgccccg
     6421 cctgcgggat tactgcatct tgtctttcag cacgcccagc atgcgttggg cggtggggct
     6481 ggtgtcgcgc gcgccattgc tgtcgagcac ggtgacctgc gtctgcgcac cgctcgaacc
     6541 gctgccgctc aggtgcacgc ggtattgcgg cgcgacggcc ggtttgacgc cgaagaggta
     6601 gcggctgaag atgttgggat cttcggggcg cacgccggta tcggtatcca ggtagcgcac
     6661 gaagtagtcg ccggcattgc ggtcgcggtc ctgcaccgag aagccgccgg aatccagcgc
     6721 cacgcccacg cggcgccagg cccggtcgaa ggcttcgccg atggtgagct cgctggcgcc
     6781 ctgcgcggtc tgcacgctct ggacgtcgac cttggcgggg ctgacctgcg cctgcgccag
     6841 cagttggcgg gcacggtcgg catcggtgcc caggaagacc atcatgcggg ccagcatggc
     6901 ggcgttcaag cccgcgtctt cctggccggg aacccagacg gccagggccg agggaccgga
     6961 gttctccatc ttctcagtca tctgctggtg gctgaggaag atctcggtat ggtcgccgac
     7021 ccgctcgacg cgcgtgcgga aacgctcgcg ctcgccgctg tcgtaggctt cgttgaaaat
     7081 cgtgcccagg acatcatgca gccagctgtc ggggatcttg gcgcggttct cggcccagtc
     7141 cgtttccagc aggccggcct gcgggttgtt ggtgttcagc gtaaagccca cgctcgtcca
     7201 gaagtcgacc agcttgggga agatctgctc gggaggatgg tcgatcacca gccagcgcag
     7261 gtcgccgtcg cgctcgatat gcatgtcgtc gcgctgcggc agcacgccgg acggcgactt
     7321 gcccgtggcc accgccgagg ctgcgtcctt gctctgctgc tggtactgcg agaacgaggt
     7381 ggcgccgccc gcatcgggcg cgcggtagcg gggatcggag gcggcctgcg tcaggtcggg
     7441 cggaatgttc agcggttcga catcactgct cgaggcgctc ttgtagtcga tgggattatc
     7501 gacgccgatt gccttgccta tgctgctgca gccggccagc tgagccaagc tcagggccaa
     7561 tgccatcgat gccgcgggct tcgcccaacg cccggcatga cgtttgttca tacgtatcct
     7621 cgtgatgttc aaagcaggcc ggtttcgctc aaggcctggc gtaccatggc atgatgcgct
     7681 gcgcccagtt cgcacagggg caggcgcaag cctagtttgg tgcggcccat ctgggcaagc
     7741 gcccatttga ccggaatggg gttggcctcg acaaacaagg ccttgttcag gcgagccagg
     7801 cgggcgttga gttcgcgcac cttgggaaca tcgcccgcca acgctgccgt gcacaggtcg
     7861 tgcatcaagc gcggcgccac gttggccgtg accgagatat tgccacgcgc gcccagcagg
     7921 atgagcgccg ccgcggtggg atcgtcgccg ctcaggatct ggaagccggc cggcgcctcg
     7981 cgcagcaaca gcgcgccgcg gccgatgtcg cccgtcgcgt ccttgacgcc gatgatgccg
     8041 ggcacctcgg ccaggcgcag cacggtttcg ttggacatgt cggccacggt gcggccgggc
     8101 acgttataga gcagcgtggg cagctccacc gcctcggcga tggtgcggaa gtggcggtac
     8161 agcccttcct gggtgggctt gttgtagtag gggacgaccg acaggccggc ctgcgcgccc
     8221 accttgagcg cgtggcgcga caagtggatg gcttcctcgg tggaattggc gcccacgccg
     8281 accacgacgg gaatgcggcc ggccgcatgc tcgaccgcca cgcggatcag ctcggcgtgc
     8341 tcgtcgatcg agacggtggg cgactcgccc gtggtgccga ccacgaccag cgcattggaa
     8401 ccctcggcca cgtgccagtc gatcagggca cggtaggcct cgtagtcgag gctgccatca
     8461 gggtgcatcg gggtcaccag ggcgaccagg ctgccctgga acaggggcgc ggcaggggta
     8521 gtagttgtca tgaaaagctc cggacccaag tgggtgggca ggccggtttt aaaccaatta
     8581 aaccaacgag tttactggat aaagcgggta accccacaca aattcctaca ggatccagcc
     8641 caccaccaaa cgccccaagg ccagcagcgg ccccaggaac agtcccagcg acccggtgac
     8701 cagcaacagc atcacgatca tcaggccata gggctcgatg cgggcgtagt gccaggccgc
     8761 ccggttcggt aacaggctga aaacgattct tccgccatcg agcggcggca cgggcagcag
     8821 gttcatcgcc atgagcacca gattcacgtt caccccggcc agcgccatct cgaaccagaa
     8881 atgctcctgc aggcccatgg ccgcgcaggc gcgcagcagc agcgcccagg ccagcgccat
     8941 ggccaggttg gccgccggcc cggccagggc cacccacagc atgtcccgct tgggacggcg
     9001 caggcggccg aaattcacgg gcaccggccg ggcccagccg aacagcacgc tcgggccgcc
     9061 cagcagcttg gcgcccagca gcatcacgat cggcaccagc agggtaccga ccgggtcgat
     9121 atgccgggcg gggttgaggc tgatgcggcc ggcctgcgcc gccgtcggat cgccgaacag
     9181 gcgtgccaca taaccatggg ccgcctcgtg cagcgtgatc gcgaagatca ggggcaaggc
     9241 atagaccgcg atggtctgga tgatttcgtt catggcgcga ttgtaggcgc agccgaagcg
     9301 gcgagactca ggccaggccc agctgcgccg gatcgccgcg ccccagccgc agcaccaccg
     9361 gctccgcgcc ggtcagatcc agcaccgtgg tgggcgcctg ggggcaggcg cccgcgtcga
     9421 ccaccgcagc cagctcgtgc gcataacgct cgacgatttc ccgggggtcg ttcagggcct
     9481 cctcttcgcc ccgggggatg agcgtggtcg acaggagggg ctcgccgatt tcctcgagca
     9541 gggccagggc gaccttgtgg tcgggcacgc ggatgccgat ggtcttgcgc gacgggtgcg
     9601 acacgcgccg cggcacctcg cgcgtagcct ccagaatgaa ggtccacggc cccggcgtgg
     9661 ccgctttgat caagcggtat tgccggttgt cgacccgcgc catcatgccg atctcggcca
     9721 ggtcgcggca caacagcgtc aggtggtgcc gttcgtccag cccgcgcagg cggcgcatgg
     9781 cgtcggcggc cgccttgtcg tcgagccggg ccaccacggc atagctcgaa tcggtgggca
     9841 cggccagcag gccgccgtcg cgcagccact gcacggcctg ccgcaacagg cggggctgcg
     9901 gattgtcagg atgtaaggtc aggagcaaag ccatgcggtt tatcctaaca ttgccgcatg
     9961 cggggccaga agacgcgggg ccaaccccgg gccgacacca ggagaagcat tcatgcgcat
    10021 ggacgatagc cgcgaaagcg acaacatcga agaccgccgc agcgccgggc cacggatcgt
    10081 cggccgcggc accatcggca tcggcaccat cgtgctggcg ctcgtggcca tgtacttcgg
    10141 ggtggatcct tccgtcgtgc tcaacctggc cgccggcggc ggccagcccg ccccccagca
    10201 gcagagcgtg cccgccggca aacccacggg cagcgatccc accgaacatt tcgtcgcccg
    10261 cgtgctgggc gagaccgagg acacctggag cgccatcttc cagcagcagt tccatcgcca
    10321 gtacagcgcc cccaccctgg tgctgttccg cggcgccacg cccacggcct gcggcaccgg
    10381 ccaggccgcc atggggccct tctactgccc gggcgacagc aaggtctacc tggacatggg
    10441 cttcttcgac gacatgtcgc gccagctcaa cgcgcccggc gactttgccc gcgcctacgt
    10501 catcgcccac gaagtcggcc accatgtgca gcacctgctg ggcatcaccg accaggtcga
    10561 cgcggcgcgc cagcgctcgc ccggccaggc caatgccttg tcggtgcggg tcgaactgca
    10621 ggccgattgc tacgccggca tctgggcgca acacgccaac cagacgcacc acatcctgga
    10681 aaacggcgac atcgagtccg gcctgaacgc cgccaaccag atcggcgacg acaccctgca
    10741 gaaacgcagc cagggctacg tagtgcccga ctcgttcacg cacggcagct cggcgcagcg
    10801 cgtacgctgg ttcaagcgcg gcttcgagag cggcgacctg cgccagtgcg acaccttcgg
    10861 cgccagccag ctctagatca cgtctgcacg cccatcaggc cgtcgacgat ggactggccg
    10921 tgcgaaacgg cagcctgcac ggcctcgcgc gggctgtaga cgaaattgcc ttgttcgaag
    10981 cagggcaccg ggtattcgtg cccggggtcc tgcacggagt tggccgccac gatctgcacg
    11041 gtggcagtga agatgggacc actggaatta ggtggagctt cttccggcgt gccgcgaagc
    11101 accttggccg tcaggcggta gcctttataa acaaaattat tttgaatcac gcaatgctct
    11161 ccttgcaatc gagcataacg tggcttcatt gcagtgcccg cactatattc gcaccgttgt
    11221 ggtcatatcg cgacaagggc aaacccggcg attccggccc caaaagcccg caaggccgcc
    11281 aaatttcatg ctaaaatcgc gcttctttac tgcggtggct gtagctcagt tggtagagtc
    11341 c
//
LOCUS       DASWPO010000102         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2612520, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000102 DASWPO010000000
VERSION     DASWPO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612520"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="VGC69_06125"
     CDS             <1..124
                     /locus_tag="VGC69_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0294896.1"
                     /translation="AWFSILDSEWPARRAAFERWLSPDNFDAAGQQRRRLGELA"
     gene            complement(167..502)
                     /locus_tag="VGC69_06130"
     CDS             complement(167..502)
                     /locus_tag="VGC69_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0294897.1"
                     /translation="MTDIHLDDGTADRYLLDAPGLSLLAFHSRTCSLCRAAREQLPGM
                     DLPVDRLCWIDAGDNGGLVERYEVFHLPALFLVRDGAFYGPVDARLDPWDLRQQIALA
                     LDNHPAELP"
     gene            740..919
                     /locus_tag="VGC69_06135"
     CDS             740..919
                     /locus_tag="VGC69_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEY0294898.1"
                     /translation="MWIILILPFIGLLWLPFYNQLEPSWLGLPFFYWYQLLWVPLTAF
                     LTWIVFRHYYKGDEE"
     gene            916..2391
                     /locus_tag="VGC69_06140"
     CDS             916..2391
                     /locus_tag="VGC69_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY0294899.1"
                     /translation="MSSSINWTALSVFIFFFVLVTVMGFAASRWQSHGSKQAALDEWG
                     LGGRRFGTWITWFLVGGDFYTAYTVIAVPALVYAVGAYGFFALPYTILVYPIVFAIMP
                     KLWSVAHKNGHVTAGDVVYASYNSRPLELAVAVTGVLATMPYIALQLVGMEVVIKALG
                     LTGEVPLFAAFIILALYTYSAGLRAPALIAFVKDLMIYIVVLAAVIIVPLKVGGYGAV
                     FSAAADAYKAKGSGSLSLAPAQFLPYASLALGSALAAFMYPHTLTGIFAAKSANTIRK
                     NAVYLPVYTLLLGLIAMLGYMAYAAHVDVKTPNDVVPALFNSVFPSWFAGFAFAAIAI
                     GALVPAAVMSIGAANLFTRNFWKAYVDPQVTPVGEAKVAKIVSLVVKLGALAFIIFVP
                     TQFAINLQLLGGVWILQTLPALVFGLFCKKLGGRALLVGWVVGMVYGTWTVFADGIKP
                     VHTIDGFTVYTGLLALVLNIIVVVVAQLVLGKKAYSAPKPA"
     gene            2563..3303
                     /gene="rlmB"
                     /locus_tag="VGC69_06145"
     CDS             2563..3303
                     /gene="rlmB"
                     /locus_tag="VGC69_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY0294900.1"
                     /translation="MASTQVLAGFHAVVARLRHAPESIRDIYIEASRRDKRMQSLLEL
                     AERAGRRVHPVAMDRLDGLAGGTRHQGVVALADERQLAVSVDDVLDVIEGPALLLLLD
                     GVTDPHNLGACLRTADAAGVHAVLAPRDRAVGLTSTVQRVACGAADTVPYITVTNLAR
                     TMRELKERDIWLVGTDDQGSDSLHAIDARRSMAWVMGAEGEGMRRLTRETCDQLVRIP
                     MLGSVESLNVSVASAVCLYETVRQRQTN"
BASE COUNT          525 a         1110 c         1085 g          610 t
ORIGIN      
        1 ggcttggttc tcgatcctcg acagcgagtg gcccgcccgc cgggccgcgt tcgagcgctg
       61 gctgtcgccc gacaacttcg acgcggccgg gcagcagcgc cgccgcttgg gcgagctggc
      121 ctgacggggc agggtcatcg ggaagcaacg agaagtcggc gcaaaatcag ggcaactcag
      181 ccgggtggtt atccagcgcc agggcgatct gctggcgcag gtcccagggg tcgaggcggg
      241 cgtcgaccgg gccgtagaag gcgccgtcgc gcaccaggaa gagggcgggc aggtggaaga
      301 cttcatagcg ctcgaccagg ccgccgttgt cgccggcgtc gatccagcac aggcggtcga
      361 ccgggaggtc catcccgggc aactgttccc gggccgcgcg gcacaggctg caggtgcggc
      421 tatggaaagc cagcagggag aggccgggcg catccagcag gtagcgatcg gccgtgccgt
      481 cgtcgaggtg tatgtcggtc attttttgct gcgtcgcaat atatctgttt cggttttgca
      541 acagtaaatc aaggatttac cccgaaaaca tcactcgctt cggagagtat ccctgtcatg
      601 cgcatgggat ttgatcgcgg gccatgcgcc caggcagtaa ggtgccgcct gtaagcgtgg
      661 aaccgctaag tgctttcttt gcgcgagacg gcgattccaa tcaatgactc ctaaatccta
      721 caaagactat acagagacta tgtggatcat tcttatattg cccttcatcg gcctgctgtg
      781 gctgcccttc tataaccagc tcgaaccttc ctggctgggc ctgccgttct tctactggta
      841 ccagctgttg tgggtgcccc tgacggcttt cctgacctgg atcgttttcc gccactatta
      901 caagggagat gaggaatgag cagctcgatc aactggaccg cgctgtccgt tttcatcttc
      961 ttcttcgtgc tcgtcacggt catgggcttt gccgcctcgc gttggcagag ccacggcagc
     1021 aagcaggccg cgctcgacga gtggggcctg ggcggccggc gcttcggcac ctggatcacc
     1081 tggttcctgg tcggcggcga cttctacacc gcctacaccg tgatcgccgt gccggccctg
     1141 gtctacgccg tgggcgccta tggcttcttc gcgctgccct acaccatcct ggtctacccc
     1201 atcgtgttcg ccatcatgcc caagctgtgg agcgtggcgc acaagaacgg ccacgtgacc
     1261 gccggcgacg tggtctacgc cagctacaac tcgcgcccgc tcgagctggc cgtggccgtc
     1321 accggcgtgc tggccaccat gccctacatc gccctgcagc tggtgggcat ggaagtggtc
     1381 atcaaggcct tgggcctgac cggcgaggtc ccgttgttcg cggcctttat catcctggcc
     1441 ctctacacct actcggcagg cctgcgcgcg cccgcgctga tcgccttcgt caaggacctg
     1501 atgatctaca tcgtggtgct ggcggccgtg atcatcgtgc cgctcaaggt gggcggctac
     1561 ggcgcggtgt tcagcgccgc ggccgacgcc tacaaggcca agggcagcgg cagcctgtcg
     1621 ctggcgcccg cgcagttcct gccctacgcc tcgctggcgc tgggctcggc cctggcggcg
     1681 ttcatgtacc cgcacaccct caccggcatc ttcgccgcca agagcgccaa caccatccgc
     1741 aagaacgcgg tctacctgcc ggtctatacg ctgctgctgg gcctgatcgc catgctgggc
     1801 tatatggcgt acgcggcgca cgtcgatgtc aaaacgccca acgacgtggt gccggcgctg
     1861 ttcaactcgg tgttccccag ctggttcgcg ggctttgcct tcgcggccat cgccatcggc
     1921 gccctggttc cggcggcggt catgtcgatc ggcgcggcca acctgttcac ccgcaacttc
     1981 tggaaggcct atgtcgaccc gcaggtcacg ccggtgggcg aggccaaggt cgccaagatc
     2041 gtctcgctgg tggtcaagct gggtgcgctg gctttcatca ttttcgtgcc cacgcagttc
     2101 gccatcaacc tgcagctgct gggcggggtg tggatcctgc agaccctgcc cgcgctggtg
     2161 ttcggcctgt tctgcaagaa gctgggcggc cgcgccctgc tggtcggctg ggtcgtgggc
     2221 atggtctacg gcacttggac ggtcttcgcc gatggcatca agcccgtgca cacgatcgat
     2281 ggcttcacgg tctacaccgg cctgctggcc ctggtgctga acatcatcgt ggtggtcgtc
     2341 gcccagctgg tgctcggcaa gaaggcctat tcggcgccca agccggcctg atcgggatcg
     2401 cgggcgggcg agcagcccgc ccgccgaaaa gcccgccgga tgcgagtccg gcgggctttt
     2461 tttcatgccg ggcggcatcg gggcggggcc gggcccgcgg cccgcgataa cattacgcgg
     2521 atacggcatt cattcattcc aagaaataaa ggttttttgc ttatggcctc gacccaagtc
     2581 ctggcgggtt ttcatgcggt ggtcgcgcgc ctgcgccacg cgcccgaatc gatccgggac
     2641 atctatatcg aagcctcgcg ccgcgacaag cgcatgcaga gcctgctcga actggccgag
     2701 cgcgccggcc ggcgcgtcca tcccgtggcc atggatcggc tcgacggcct ggccggcggc
     2761 acgcggcacc agggcgtggt ggcgctggcc gacgagcgcc aactggccgt gagcgtcgac
     2821 gacgtgctcg acgtcatcga aggcccggcg ctgctgctgc tgctcgacgg cgtgaccgac
     2881 ccgcacaacc tgggcgcctg cctgcgcacg gccgacgccg ccggcgtgca cgccgtgctc
     2941 gccccgcgcg accgcgccgt gggcctgacc agcaccgtgc agcgcgtggc ctgcggcgcg
     3001 gccgacaccg tgccctacat caccgtcacc aacctggccc gcaccatgcg cgagctcaag
     3061 gaacgcgaca tctggctggt cggcaccgac gaccagggca gcgacagcct gcacgccatc
     3121 gacgccaggc gatccatggc ctgggtcatg ggcgccgaag gcgagggcat gcgccgcctc
     3181 accagggaaa cctgcgacca gttggtgcgc atccccatgc tgggctcggt ggagagcctg
     3241 aacgtcagcg tggccagcgc cgtctgcctg tacgaaacgg tgaggcagcg ccagacgaat
     3301 tgaaccgcag caaccagaga tgaattgagc
//
LOCUS       DASWPO010000103         8621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_380053, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000103 DASWPO010000000
VERSION     DASWPO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380053"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGC69_06150"
     CDS             complement(<1..339)
                     /locus_tag="VGC69_06150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0294901.1"
                     /translation="MTAAAQQRTILIMAGGTGGHIMPGLAVADVLRSRGWRVLWLGNP
                     EKMEGRLVPARGFDLVPLRFAGVRGRGLGVLLRLPLTLGRALGQAWMRLSQVRPDVVL
                     GMGGYVAFPGG"
     gene            complement(336..1535)
                     /gene="ftsW"
                     /locus_tag="VGC69_06155"
     CDS             complement(336..1535)
                     /gene="ftsW"
                     /locus_tag="VGC69_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY0294902.1"
                     /translation="MSTTAYTGPAASVNAVRPGRTRMRNFDLPLVLAVAALLMLGLLM
                     VYSASIALGDGPRYAAYGRYYFILRHGIFISIGVMVAIVTLTIPVQAWQKVAVPLFMV
                     SLALMVVVLVPGIGREVNGARRWIPLGPINFQPSELMKLAAVIYAANYTVRKQEYMQV
                     FARGFLPMACALGAVGMVLLLEPDLGAFMVIVAIAIGVLFLGGINGKYFSSLLGVLIG
                     LFLMLIWLSPWRRARLFAYLDPWNEANAYGSAYQLSHSLIAFGRGEWLGVGLGASVEK
                     LHYLPEAHTDFLMAVVGEELGFAGVMLVIALFGVVVQRGFDIGRQAIAMERTFAGLAA
                     HGVALWFGVQAFINMGVCLGLLPTKGLTLPLMSYGGSGIVMNLCGLALLLRVDIENRI
                     LMRGGRI"
     gene            complement(1612..3150)
                     /gene="murD"
                     /locus_tag="VGC69_06160"
     CDS             complement(1612..3150)
                     /gene="murD"
                     /locus_tag="VGC69_06160"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY0294903.1"
                     /translation="MNDMQTVRDDAPLVLILGLGETGVAAARWCAREGAGLRVADTRA
                     EPGGLAELRAALESGGHAVDYRLGCGAVFDTALLDGVSQLVLSPGLAPGQEPARSLLA
                     AAAERGVEIVGEIELFARALARLAETRAYRPRLLAVTGTNGKTTVTSLTRHLVEACGL
                     SARVAGNISPAALTALMDALDGDSLPQVWVIELSSFQLETTRSLAADASVVLNVTQDH
                     LDWHGSMQAYAQAKARLLGMARVAIVNRDDPLTCAMVPSLQALPVRSFGRDLPELVGD
                     MGLDLGQGVSWLTSCEPSEFDEAVAAPVRRKKDAPEPVRAAGRLNRLMPMDALRIRGL
                     HNAMNALAALQLARALDLGWGPMLRALRDYAGEPHRATFVRSVGGVDYINDSKGTNVG
                     ATVAALEGLGQSVVLIAGGQGKGQDFSPLVAPVSRHARAVVLIGEDGPAIGQALAASG
                     VSCVPAGSMREAVRRAAELARPGDAVLLSPACASFDMFRGYPHRGQVFAADVEELALD
                     RGEV"
     gene            complement(3160..4329)
                     /gene="mraY"
                     /locus_tag="VGC69_06165"
     CDS             complement(3160..4329)
                     /gene="mraY"
                     /locus_tag="VGC69_06165"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY0294904.1"
                     /translation="MLLELFRALSDNMRVLSVFEYITLRAVLACATSLLIGLVAGPKV
                     IRKLTELKIGQAVRSYGPQSHLVKTGTPTMGGALILISIAISTLLWADWTNRFVWVVL
                     LVTFGFGWIGWADDYRKVVHRDPEGMPARQKFFWQALIGVVAAVYLAFAVSAPANTEL
                     WPLFKAWVESGFAMALPTRADLIVPFFKSVSYPLGVLGFVVLTWAVIVGTSNAVNLTD
                     GLDGLAIMPTVMVGAALGIFAYVVGRVDYSRYLLFPYIPGASELMVLCAAIGGAGLAF
                     LWFNTYPAQVFMGDVGALALGGALGTIAVIVRQEIVLFIMGGVFVVETLSVMMQVTWF
                     KYTKRRYGEGRRIFRMAPLHHHFEVGGWKETQVVVRFWIITMMLVLVGLSTLKLR"
     gene            complement(4319..7141)
                     /gene="murF"
                     /locus_tag="VGC69_06170"
     CDS             complement(4319..7141)
                     /gene="murF"
                     /locus_tag="VGC69_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase
                     MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine
                     ligase MurF"
                     /protein_id="HEY0294905.1"
                     /translation="MNARPTRPQGPAAGTAQVLAWLRAQVAGQAHLQLDSREIRAGDV
                     FVACAGLRSDGRHYIAQALAHGAGAVIYEADGAEATADNLGAALAVQGLRAMLGELAD
                     QWYGQPSAALSVVAVTGTNGKTSCVQWIADALTRLGRPCGTLGTLGARLPDGRSLGGT
                     LTTPDVLSVHRTLAAMRDAGVAAVALEASSIGLEQGRLDGVRIKVAGYTNLTRDHLDY
                     HGTMERYEASKAMLFQRAGLSAAIINADDAAGRRLIDGLPAGLARSYALDDGKAGIRA
                     RDVHSVAQGQVFTLAMPGGETQIITRLLGRHNVYNLLLVAGVLSALGFPLSQISRQLS
                     EAEPVDGRMQIVDPLPVAEPAAGPMVVVDYSHTPDSLERALAALRPVVQARGGKLVCV
                     FGCGGERDAGKRPQMGRIAAVGADRIIVSSDNPRGEAPQAIIEQVLLGVPVGARIEIQ
                     ADRAQAILRAIWSAAAEDVVLLAGKGHETYQEIDGRKLPFDDREWARLALLLPRVPAL
                     STDTRRIGHGELFLALSGDSFDGHDYLQQAQASGACAAVVARRVADCDLPQLVLGDTR
                     LALGRIGAVWRARFDLPVVAVTGSNGKTTTKEMVSAILAAWQGEENRLATAGNFNNDI
                     GVPLTLLRLRPRHRAAVFELGMNHPGEIACLAEMASAGVALVNNAQREHQEFMHSVEA
                     VARENGAAIAALPLDGVAVYPGDDAYTGVWDELAGSRRKLRFGLAPGAEVYAEHIESD
                     VSGSRCDVVTPLGRAALRLPVPGRHNLRNALAAIACGLAAGASLDTAVRALASFNAVN
                     GRMQRKAMDGGAVLIDDTYNANPDSVRAAIDVLATLAGPRALVLGDMGEVGGNGPAMH
                     REVGEYARGRGIDVLFTLGEATRLSAEAFGPGAHACGSVEEIVAGLRKQAYASILVKG
                     SRFMRMERVVQGLTSNNQGAGGTHAA"
     gene            complement(7138..>8621)
                     /locus_tag="VGC69_06175"
     CDS             complement(7138..>8621)
                     /locus_tag="VGC69_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0294906.1"
                     /translation="AIWAIPDDVKQANPGQIKALTGLLDVPEADLRRRLNDEDRNFVY
                     LKRQVPMDVADKIKQLNIPGVHQQPESMRYYPEGDVAAHVVGFTDIEDKGQEGVELTF
                     NDQLSGRDGSRRVIKDRLGHVIEDVQAVNPPEDGRDLTLSIDTRLQYLLYKELQDAIT
                     QHQARAATAVILDVQTGEILALASLPAFDSNHREKLQADALRNRAVTDTFEPGSIMKP
                     FTAALALDLGRITTSTLFETGNGRFQYQGNTITDVERGGNGTIDVAGVLRLSSNIGMT
                     LISERLEAHEMWDCFASLGLGQAPQVGFPGMAPGRLRPWDRWRLIEKATMAYGYGLSV
                     SLLQAARAYTVFARDGDMVSLSLVKRDSASAPTSVRIYSSKTAGLIRSMLEAAAGPNG
                     SKVTVPGYRIAGKTGTARKIVDGRYSQQRYRSSFVGFAPVSNPRIVVAVSVDEPKVGG
                     YYGAAVAGPVFSRVMSGSLRLMGVQPDAPFESTVVAANPEPAR"
BASE COUNT         1411 a         3075 c         2933 g         1202 t
ORIGIN      
        1 gccgcccggg aacgccacgt agccgcccat gcccagcacc acgtcgggac gcacctgcga
       61 caggcgcatc caggcctggc ccagggcccg gcccagcgtc agcggcaggc gcagcagcac
      121 gcccaggccg cggccgcgca cgccggcaaa gcgcagcggc accaggtcga agccgcgggc
      181 cggcaccagc ctgccttcca ttttttcggg gttgcccagc cacagcacgc gccagccgcg
      241 cgaacgcagc acatcggcca cggccaggcc gggcatgatg tgcccgccgg tgccgccggc
      301 catgatgagg atggtgcgct gctgcgcggc cgccgtcata tccgcccccc gcgcatcagg
      361 atgcggttct cgatgtcgac ccgcagcagc agcgccagcc cgcacagatt catcacgatg
      421 cccgagccgc cgtagctcat cagcggcagg gtcagcccct tggtgggcag caggccgagg
      481 cacacgccca tattgatgaa ggcctgcacg ccgaaccaca aggccacgcc atgcgcggcc
      541 aggccggcga aggtgcgttc catggcaatg gcctgccggc cgatgtcgaa gccgcgctgc
      601 accaccacgc cgaatagcgc gatcaccagc atcacgccgg cgaaacccag ctcttcgccg
      661 accacggcca tcaggaagtc cgtatgggct tccggcaggt agtgcagttt ctcgacgctg
      721 gcgcccaggc ccacgcccag ccattcgccg cgcccgaagg cgatcagcga gtgcgacagc
      781 tgataggcgc tgccgtaggc attggcctcg ttccaggggt ccagataggc gaacagacgc
      841 gcccggcgcc agggcgacag ccagatcagc atcaggaaca gcccgatcag cacgcccagc
      901 aggctgctga aatacttgcc gttgatgccg ccaaggaaca gcacgccgat ggcgatcgcc
      961 acgatcacca tgaaagcgcc caggtcgggc tcgagcagca gcaccatgcc caccgcgccc
     1021 agggcgcaag ccatgggcag gaagccgcgc gcgaagacct gcatgtactc ctgcttgcgc
     1081 accgtgtaat tggcggcata gatcacggcg gccagcttca tcagctcgga gggctggaag
     1141 ttgatgggcc ccagcggaat ccagcggcgg gcgccgttga cctcgcggcc gatgccggga
     1201 accagcacga ccaccatcag cgccagcgac accatgaata gcggcacggc gaccttttgc
     1261 caggcctgga cgggaatggt cagcgtcacg atggccacca tcacgccgat ggaaatgaag
     1321 atgccatggc gcaggatgaa gtagtaccgg ccgtaggcgg cgtagcgcgg accgtcgccc
     1381 agcgcgatcg acgccgaata caccatcagc aggcccagca tcagcagggc ggccaccgcc
     1441 agcaccagcg gcaggtcgaa gttgcgcatg cgcgtgcgcc cggggcgcac ggcattcacg
     1501 ctggcggcag gtccggtata ggcggtagtg ctcatagtga ggcccccttg gcgctacgcg
     1561 ccttcccccc gggggggagc gatgcgcgct ggaggcggct cggcgtgcgc atcagacttc
     1621 tcccctgtcc aaagccaatt cctcgacgtc ggccgcaaag acctggccgc ggtgcggata
     1681 gccgcggaac atgtcgaaac tggcgcaggc cggcgatagc agcacggcat cgcccggccg
     1741 ggcgagctcg gcggcgcggc gcaccgcttc ccgcatggaa cccgccggca cgcaggacac
     1801 gccgctcgcg gccagcgcct ggccgatcgc ggggccgtcc tcgccgatca gcaccacggc
     1861 gcgcgcatgg cgggacacgg gcgccaccag cggcgagaag tcctgcccct tgccctgccc
     1921 gcccgcgatc agcaccacgc tctggcccag cccttccagc gcggccaccg tggcgcccac
     1981 attggtgccc ttgctgtcgt tgatgtagtc cacgccgccc acgctgcgca cgaaagtggc
     2041 gcgatgcggt tcgccggcgt agtcgcgcag ggcccgcagc atggggcccc agcccaaatc
     2101 cagcgcgcgc gccagctgca gcgcggccag ggcgttcatg gcattgtgca ggccgcggat
     2161 gcgcaacgcg tccatcggca tcaggcgatt gaggcgaccg gcggcgcgca ccggctcggg
     2221 cgcgtctttc ttgcggcgca ccggcgccgc cacggcctcg tcgaactcgc tgggctcgca
     2281 gctggtcagc caggacacgc cctggccgag gtccagcccc atgtctccca ccagctcggg
     2341 caggtcgcgg ccgaagctgc gcaccggcaa ggcctgcagg gacggcacca tggcgcaggt
     2401 gagcgggtcg tcgcgattga cgatggccac gcgggccatg cccagcaggc gcgccttggc
     2461 ttgcgcatag gcttgcatgc tgccgtgcca atccaggtgg tcctgcgtca cgttgagcac
     2521 cacggaggca tcggccgcca gcgaccgggt ggtttcgagc tggaagctcg acagctcgat
     2581 cacccacacc tgcggcaggc tgtcgccatc gagcgcgtcc atcaacgcgg tcagcgcggc
     2641 cgggctgata ttgcccgcca cgcgcgccga caggccgcaa gcctcgacca ggtgccgggt
     2701 cagcgacgtg accgtcgtct tgccgttggt gccggtcacg gccagcagcc tgggacggta
     2761 tgcgcgcgtc tcggccagcc tggccagggc gcgggcgaac aattcgatct cgcccacgat
     2821 ctcgacgccc cgctcggccg cggcggccag cagggaacgc gcgggttcct gcccgggggc
     2881 caggccgggg ctgagcacca attggctcac gccgtcgagc aaggccgtat cgaacaccgc
     2941 gccgcaaccc aggcggtagt cgacggcatg gccgccgctt tccagcgccg cgcgcagctc
     3001 ggccagcccg cccggttcgg cgcgagtgtc cgccacgcgc agcccggcac cctcgcgggc
     3061 gcaccagcgc gcggcggcca cgcccgtctc gcccaatccg aggatcagga cgagcggcgc
     3121 atcgtcacgg acggtttgca tgtcgttcat cgcaatactt caacgcagct tcaaggtgga
     3181 aagaccgacc agcaccagca tcatggtgat gatccagaac cgcacgacga cctgggtttc
     3241 cttccagccg ccgacttcga aatggtggtg cagcggcgcc atgcggaata tccgccggcc
     3301 ctcgccatac cggcgcttgg tgtacttgaa ccaggtcacc tgcatcatca ccgacagcgt
     3361 ctcgaccacg aacacgccgc ccatgatgaa aagcacgatt tcctggcgca cgatcacggc
     3421 gatggtgccc agcgcgccgc ccagggccag cgcgcccacg tcgcccatga acacttgcgc
     3481 cgggtaggtg ttgaaccaca ggaaggccag ccccgccccg ccgatcgccg cgcacagcac
     3541 catcagctcg gacgcgcccg ggatataggg aaacagcagg tagcgcgagt aatcgacgcg
     3601 gcccaccaca taggcgaaga tgcccagcgc ggcgcccacc atcaccgtcg gcatgatggc
     3661 caggccgtcg aggccgtcgg tgaggttgac cgcattgctg gtgcccacga tcacggccca
     3721 ggtcagcacc acgaagccca gcacgcccag cgggtagctc acgctcttga agaaaggcac
     3781 gatcaggtcg gcgcgcgtgg gcagcgccat ggcgaaaccg ctttccaccc aggccttgaa
     3841 caggggccag agctcggtat tggccggcgc cgacacggca aaggccaggt agacggcggc
     3901 cacgacgccg atcagcgcct gccagaagaa cttctggcgg gcgggcatgc cctcgggatc
     3961 gcgatgcacc accttgcggt agtcgtcggc ccagccgatc cagccgaagc cgaaggtgac
     4021 cagcagcacc acccagacga accggttggt ccagtcggcc cacagcaggg tgctgatggc
     4081 gatcgaaatc aggatgagcg cgccgcccat ggtgggggtg ccggtcttga ccaggtggct
     4141 ctgcggcccg tagctgcgca cggcctggcc gatcttcaat tcggtgagct tgcggatcac
     4201 tttcggaccg gccaccaggc cgatcagcag ggacgtggcg caggccagca cagcgcgcag
     4261 ggtgatgtac tcgaacacgc tgagcacgcg catgttatcg gacagcgccc ggaaaagctc
     4321 aagcagcatg cgtgcctccc gcgccctgat tgttggaagt cagcccctgc accactcgct
     4381 ccatgcgcat aaaacgcgaa ccctttacca agatgctggc gtaggcctgc ttgcgcagcc
     4441 ccgccacgat ttcttccacc gagccgcagg cgtgcgcgcc cggcccgaac gcctcggcgc
     4501 tcaggcgcgt ggcctcgccc aacgtgaaca ggacgtcgat gccgcggccc cgggcatatt
     4561 cgcccacctc gcggtgcatg gccgggccgt tgccgccgac ttcgcccatg tcgcccagca
     4621 ccagcgcccg cgggccggcc agcgtggcca gcacgtcgat ggcggcccgg accgagtcgg
     4681 ggttggcgtt gtaggtgtcg tcgatcagca cggcgccgcc gtccatggcc ttgcgctgca
     4741 tgcggccgtt gaccgcgttg aacgacgcca gcgcccgcac ggcggtatcc agcgaagcgc
     4801 cggccgccag gccgcaggcg atggcggcca gggcgttgcg caaattgtgc cggccaggaa
     4861 cgggcagccg cagcgcggcg cgccccagcg gggtgacgac gtcgcagcgc gagcccgaga
     4921 catcgctctc gatatgctcc gcgtatacct cggcaccggg cgccaggccg aagcgcagct
     4981 tgcggcgcga gccggccagt tcgtcccaga cgccggtata ggcgtcgtcg ccgggataga
     5041 ccgccacgcc gtccaggggc aaagcggcga tggcggcgcc gttctcgcgc gccacggcct
     5101 cgacggaatg catgaattcc tgatgctcgc gctgggcatt gttgaccagg gccacgccgg
     5161 ccgaggccat ctcggccagg caggcgattt cgcccggatg gttcatgccc agctcgaaca
     5221 cggcggcgcg atgccgcggc cgcaggcgca gcagcgtcag cggcacgccg atgtcgttgt
     5281 tgaaattgcc ggccgtggcc aggcggtttt cctcgccctg ccaggcggcc aggatggccg
     5341 acaccatttc cttggtcgtg gtcttgccgt tgctgccggt gacggcgacc accggcaagt
     5401 caaagcgggc acgccagact gcgccgatgc ggcccagcgc caggcgcgta tcgcccagga
     5461 ccaattgcgg cagatcgcaa tcggccacgc ggcgcgccac cacggcggcg caggcgccgg
     5521 atgcctgggc ttgctgcagg tagtcgtggc cgtcgaagct gtcgcccgac aacgccagga
     5581 acagttcgcc gtggccgatg cggcgcgtgt ccgtcgacag cgcgggaacc cggggcagca
     5641 gcaaggccag gcgcgcccat tcgcggtcgt cgaagggcag cttgcggccg tcgatttcct
     5701 ggtaggtttc atggcccttg ccggccagca gcacgacatc ctcggcggcg gcggaccaga
     5761 tggcccgcag gatggcctgg gcccggtcgg cctggatctc gatccgggcg ccgaccggca
     5821 cacccagcaa gacctgctcg atgatggcct gcggcgcttc gccgcgcggg ttgtcgctgc
     5881 tgacgatgat gcgatcggcg ccgacggcgg cgatgcggcc catctggggc cgcttgcccg
     5941 cgtcgcgctc gccgccgcag ccgaacacgc acaccagctt gccgccgcgc gcctggacga
     6001 ccggccgcag ggccgcgagg gcgcgttcga gcgaatcggg cgtgtgcgaa taatcgacca
     6061 cgaccatcgg ccccgccgcc ggctcggcca cgggcagcgg atcgacgatc tgcatgcggc
     6121 cgtccaccgg ctcggcttcg ctcagctggc gggaaatctg cgacagcgga aagcccagcg
     6181 cggacagcac gccggccacc agcagcaggt tgtagacatt gtgccggccc aggagccgcg
     6241 tgatgatctg cgtctcgccg cccggcatgg ccagcgtgaa cacctggccc tgcgccaccg
     6301 aatgcacgtc ccgcgcgcgg atgcccgcct tgccgtcgtc cagcgcatag ctgcgcgcca
     6361 ggccggcggg caggccgtcg atcaggcggc ggccggccgc atcgtcggca ttgatgatcg
     6421 cggcggacaa accggcgcgc tgaaagagca tggccttgga cgcctcgtag cgctccatgg
     6481 tgccgtgata gtccaggtga tcgcgcgtca ggttggtgta gcccgccacc ttgatgcgca
     6541 cgccgtccag gcggccctgc tccagcccga tggacgaagc ctccagcgcc acggcggcaa
     6601 cgccggcatc gcgcatggcc gccagggtgc ggtgcacgga cagcacgtcg ggcgtcgtca
     6661 gcgtgccgcc cagcgaccgg ccgtcaggca gcctggcgcc cagggtgccc agggtgccgc
     6721 aaggccggcc caggcgcgtc agggcatcgg cgatccattg cacgcacgac gtcttgccgt
     6781 tggttcccgt gacggccacc acgctcaagg ccgcggaagg ctgcccgtac cattggtcgg
     6841 ccagctcgcc gagcatggcg cgcaggccct gcacggccag ggcggcgccc aggttgtcgg
     6901 cggtcgcttc ggcgccgtcg gcttcataga tcacggcgcc ggcgccgtgc gccagggcct
     6961 gggcgatata gtgccgcccg tcgctgcgca ggcccgcgca ggccacgaac acatcgcccg
     7021 cgcggatctc gcgggaatcg agctgcaggt gcgcctggcc ggccacctgc gcgcgcagcc
     7081 aggccagcac ctgggccgtg ccggccgcgg ggccttgggg ccgggtcggg cgggcgttca
     7141 tcgcgcgggc tccggattgg ccgccacgac ggtcgactcg aacggcgcgt cgggctgcac
     7201 gcccatcagg cgcaggctgc cgctcatcac gcgcgagaac accggccccg ccacggcggc
     7261 gccgtaatag ccgccgacct tgggctcatc gacggacacc gccacgacga tgcgaggatt
     7321 ggaaaccggc gcgaagccga cgaacgagct gcggtagcgc tgctggctgt agcggccgtc
     7381 gacgatcttg cgggccgtgc cggtcttgcc cgcgatgcgg tagcccggca ccgtgacctt
     7441 ggagccgttg gggccggcgg ccgcctccag catgctgcgg atcagcccgg ccgtcttgga
     7501 agaatagatc cgcacgctgg tcggcgcgct ggcgctgtcg cgcttgacca gcgacagcga
     7561 caccatgtcg ccgtcgcgcg cgaacaccgt ataggcgcgc gcggcctgca gcagcgacac
     7621 ggacaggccg tagccgtagg ccatggtggc cttttcgatc aggcgccagc ggtcccaggg
     7681 gcgcagccgg cccggcgcca tgcccgggaa acccacctgg ggcgcctggc ccaggccgag
     7741 cgaggcgaaa caatcccaca tctcatgcgc ttccagccgt tccgagatca gcgtcatgcc
     7801 gatgttgctg gacaggcgca gcacgcccgc cacatcaatc gtgccgttgc cgccgcgttc
     7861 gacgtcggtg atcgtgttgc cctggtactg gaacctgcca ttgccggtct cgaacaaagt
     7921 actcgtggtg atgcggccca gatccagggc cagcgccgcc gtaaacggct tcatgataga
     7981 accgggctcg aacgtatccg taaccgcccg gttacgcagc gcgtccgcct gcagtttttc
     8041 gcggtggttc gaatcgaagg cgggcaggct ggccagggcc aggatctcgc ccgtctgcac
     8101 atccaggatc acggccgtgg cggcgcgcgc ctggtgctgg gtgatggcgt cctgcaattc
     8161 tttatagagc aggtactgca ggcgcgtgtc gatcgacagg gtcaggtcgc ggccgtcctc
     8221 cggcggattc accgcctgca cgtcctcgat cacgtggccg agccggtcct tgatcacgcg
     8281 gcggctgccg tcccggccgg acagctggtc gttgaaggtg agctcgacgc cttcctggcc
     8341 cttgtcctcg atatcggtga aacccaccac gtgcgccgcc acgtcccctt cggggtagta
     8401 gcgcatggat tcgggctgct ggtgcacgcc gggaatgttg agctgcttga tcttgtcggc
     8461 cacgtccatc ggcacctggc gcttcaggta gacgaagttg cggtcctcgt cgttgaggcg
     8521 gcggcgcagg tcggcctccg gcacgtccag caggccggtc agggccttga tctgcccggg
     8581 gttggcctgc ttgacgtcgt cgggaatcgc ccagatggcg c
//
LOCUS       DASWPO010000104         7787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_914686, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000104 DASWPO010000000
VERSION     DASWPO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914686"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VGC69_06180"
     CDS             complement(<1..560)
                     /locus_tag="VGC69_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0294907.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLSNKFGGEA
                     KGYDQIDATPEEKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
                     YDFPGDDTPIIKGSAKLALEGDTGELG"
     gene            complement(617..2719)
                     /gene="fusA"
                     /locus_tag="VGC69_06185"
     CDS             complement(617..2719)
                     /gene="fusA"
                     /locus_tag="VGC69_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0294908.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYGVPRLAFVNKMDRTGANFFKVYDQLKT
                     RLRANPVPIVIPIGAEESFTGVVDLVKMKAILWDEASQGTKFSYVDIPAELQASADEW
                     HEKLVEAAAESTEELMNKYLETGSLDEAEINLAIRQRTIAGEIQPMLCGTAFKNKGVQ
                     RMLDAVIDYLPSPVDIPPVDGQDDEGNAVQRNADDGEKFSALAFKLMSDPFVGQLTFV
                     RVYSGVLKSGDTVYNPIKGKKERIGRILQMHANNREEIKEVLAGDIAAVVGLKDVTTG
                     ETLCDVDSHILLERMVFPEPVISQAVEPKSKADQEKMGLALSRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEILVDRMKREFGVEANVGKPQVAYRETIRKTCEEVEGKFVKQSG
                     GRGQYGHVVLKVEPLPPGGGYEFVDAIKGGVVPREFIPAVDKGIQETLPAGILAGYPV
                     VDVKATLFFGSYHDVDSNENAFKMAGSMAFKEGMRRASPVLLEPMMAVEVETPEDYAG
                     TVMGDLSSRRGMVQGMDDIVGGGKIIKAEVPLAEMFGYSTSLRSATQGRATYTMEFKH
                     YAEAPKNVADEVIAARGK"
     gene            complement(2739..3209)
                     /gene="rpsG"
                     /locus_tag="VGC69_06190"
     CDS             complement(2739..3209)
                     /gene="rpsG"
                     /locus_tag="VGC69_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0294909.1"
                     /translation="MPRRREVPKREILPDPKFGSVELAKFMNVVMLDGKKAVAERIVY
                     GAFEQVQSKTGKEPLEVFSLAINNIKPIVEVKSRRVGGANYQVPVEVRPVRRLALAMR
                     WLREAAKKRGEKSMDLRLAGELIDASEGRGAAMKKREDTHKMAEANKAFSHFRW"
     gene            complement(3394..3771)
                     /gene="rpsL"
                     /locus_tag="VGC69_06195"
     CDS             complement(3394..3771)
                     /gene="rpsL"
                     /locus_tag="VGC69_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY0294910.1"
                     /translation="MPTISQLVRKPREVSIIKSKSPALENCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQSRSKYGAKRPKKA"
     gene            4485..5225
                     /locus_tag="VGC69_06200"
     CDS             4485..5225
                     /locus_tag="VGC69_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0294911.1"
                     /translation="MKIASLEDDLNQARQIQQVLASAGYGSDSFQQSVDLLSAMRSTT
                     YDLVLLDWQLPDMNGDEVLRQLRGHFGYAIPVILLTSRSQEDDLVQGLQAGADDFISK
                     PLRPAELLARISALLRRSQPAQSAQSAFTVGAYHIDPVERLIKLDSRNIVLAPKEFDL
                     AVLFFRNIGRLLSRDVLAESVWNREIPATSRTLDTHLSNIRQKLQLRAQHGVRLSSSY
                     ALGYRLDLVQVAEHETTSDRVSPFPIVV"
     gene            5253..7592
                     /locus_tag="VGC69_06205"
     CDS             5253..7592
                     /locus_tag="VGC69_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY0294912.1"
                     /translation="MVETRQRRRRFEWSADWLLIWLLVAGAALLGALNGLGRFDQGLY
                     DQAMAASSRPASPDILLVAVDDESLAALGPWPWPRSLHAQLLDRLHDARAVGLAVLYS
                     EFDASNPQDNALLAEAIKRHGRVVLPATLNHLPTPTRYDTADSTLAEAAAGTGFLNTL
                     PDDDGIIRRVQWRAKLDNRTCLHMALAILAAGGEQDKVDVMLRQAGPTGQIGIAYAGP
                     PGRLRTVSYLQVLRGQVPADWIRGKYVLVGSQASGLSRMFPTPVSHQFNGMSGAELLG
                     ELLQAARDDVMIRTAPAWFTTLYTALSVLLLCLALRHMAPRRALLASIAAVALVLAIS
                     FLAMNYLHYWVAPAATLFMLVLCYPLWCWRQQEAILGHMDSELQRLRQEYPPILSEAQ
                     LPSRLGKSAMELRLRELHQALTRVRNLRRFLTDGFDGIADPTFVSDKDGHLRLRNRAA
                     LAYFRDLSLRAPRLGQSTAWLLEQLVSDPAVLDRVGPALTGHQPDTSPWRLDMEIQDR
                     QGRSIILKGAPIHTSTGEFAGTVVTFNDISAIRQAERKREETLRFVSHDMRAPQNSIL
                     ALVYLNSDETDPQKQRDAWSRIGQLARRTLRLVDDFVHLTRAESVRITHAVIDLGMLV
                     HEALDDFWALAQTRRVDLSIDGDLTEALICGDRALLLRAVCNLIDNALKYTPSGGRVH
                     VSLQRDGSSWLVRIVDSGTGIAAEDIPQIFQPFSRVGPTARNDNNGAGLGLAFVNTVA
                     WRHGGSVGVNSLPGAGSTFTLQLPAYTEEEAALATERAA"
BASE COUNT         1300 a         2598 c         2403 g         1486 t
ORIGIN      
        1 cccagctcgc ccgtgtcgcc ttcgagggcc agcttcgccg agcccttgat gatcggggtg
       61 tcgtcgcccg ggaagtcata cttcgacagc agctcgcgca cttccatttc caccagctcg
      121 agcagctcgg cgtcgtccac catgtcgcac ttgttcagga acacgatgat gtacggcacg
      181 cccacctgac gcgccagcag gatgtgctcg cgcgtttgcg gcatcgggcc gtccgcggcc
      241 gaacacacca ggatcgcgcc gtccatctgc gcggcgcccg tgatcatgtt cttcacgtag
      301 tcggcgtggc ccgggcagtc aacgtgcgcg tagtgacggt tggccgtctc gtactcgacg
      361 tgcgccgtgt tgatcgtgat gccgcgagcc ttttcttcag gagtcgcatc gatctggtcg
      421 tagcccttgg cttcgccacc gaacttgttc gacagaacgg tcgtgatagc tgccgtcaac
      481 gtcgttttgc cgtggtcaac gtgaccaatc gtacccacgt tcacgtgcgg cttggtacgt
      541 tcaaacttgc cttttgccat gatctctatc cttcaagtgc aaggagaaaa tgttgttcgg
      601 ttaactcgga aagatattac ttgccccgag cggcgatgac ttcgtcagcg acgttcttgg
      661 gcgcttcggc gtaatgcttg aattccatcg tgtaggtggc acggccctgg gtcgcggaac
      721 gcagcgaggt cgagtagccg aacatctcgg ccaggggaac ctcggccttg atgatcttgc
      781 cgccgccgac gatgtcgtcc atgccctgca ccatgccgcg gcgggacgac aggtcgccca
      841 tcacggtacc cgcgtagtcc tcaggcgttt cgacttcaac ggccatcatg ggctcgagca
      901 gcacggggct ggcacggcgc atgccttcct tgaaggccat cgacccggcc atcttgaacg
      961 cgttttcgtt cgagtccaca tcgtggtagg agccgaagaa cagcgtggcc ttgacgtcca
     1021 ccaccggata accggccagg atgccggcag gcagggtttc ctggatgccc ttgtccaccg
     1081 cggggatgaa ctcgcgcggc accacgccgc ccttgatggc gtcgacgaac tcgtagccgc
     1141 cgcccggagg caggggttcg accttgagca ccacgtggcc gtactggccg cggccgcccg
     1201 actgcttgac gaacttgcct tcgacttctt cgcaggtctt gcggatggtt tcgcggtagg
     1261 ccacctgggg cttgccgacg ttggcttcca cgccgaattc gcgcttcatg cggtcgacca
     1321 ggatttccag gtgcagctcg cccatgccgg agatgatggt ctggccggat tcctcgtcgg
     1381 tgcgcacgcg gaacgacggg tcttcctgcg ccaggcgcga cagcgccagg cccatctttt
     1441 cctggtcggc cttggacttg ggttcgacgg cctgcgagat cacgggctcg gggaacacca
     1501 tgcgttcgag caggatgtgg gaatcgacgt cgcacagcgt ttcgccggtc gtcacgtcct
     1561 tcaggcccac cacggcggcg atgtcgcccg ccagaacttc cttgatttct tcgcggttgt
     1621 tggcgtgcat ctgcagaatg cggccgatgc gttccttctt gcccttgatg gggttgtaga
     1681 cggtgtcgcc cgacttcagg acgcccgagt acacccgtac aaaggtcagc tggcccacga
     1741 acggatcgct catcagcttg aatgccaggg cggagaactt ttcaccgtcg tcggcgttgc
     1801 gttggaccgc gttgccctcg tcgtcctggc cgtcgaccgg gggaatgtcc acgggcgaag
     1861 gcaggtagtc gatgacggcg tccagcatgc gctgcacgcc cttgttcttg aacgcggtgc
     1921 cgcacagcat gggctggatt tcgccggcga tggtgcgctg gcggatggcc aggttgatct
     1981 cggcttcgtc gagcgaaccg gtctcgaggt acttgttcat cagctcttcg gtcgactcgg
     2041 cggcagcctc gaccagcttc tcgtgccatt cgtcggcgga agcctgcagt tcggccggga
     2101 tgtcgacata gctgaacttg gtgccctggc tggcttcgtc ccagagtatg gccttcatct
     2161 tgacgaggtc gaccacgccc gtgaaggatt cttcggcgcc gatcgggatc acgatgggca
     2221 cgggattggc gcgcaggcgc gtcttcagct ggtcatagac cttgaagaag ttggcgccgg
     2281 tgcggtccat cttgttgacg aaggccaggc ggggcacgcc gtacttgttg gcttggcgcc
     2341 acacggtttc ggactggggc tgcacgccac ccacggcgca atacaccatg caggcgccgt
     2401 cgagcacgcg catggaacgt tccacctcga tggtgaagtc cacgtggccc ggggtgtcga
     2461 tgatgttgat gcggtgctcg gggtagttgc ccgccatgcc cttccagaac gcggtggtgg
     2521 ccgcggacgt gatggtgatg ccgcgctctt gctcttgttc catccagtcc atggtggcgg
     2581 cgccatcgtg gacttcgcca atcttgtgat tgacgccggt gtagaacagg atgcgctcgg
     2641 tcgtggtggt cttccctgca tcgatgtgcg cagagatgcc aatgttgcga tagcgctcga
     2701 tcggggtttt gcgggccatg gtagggtgat tccttaaatt accaacggaa atggctgaag
     2761 gccttgttgg cttcggccat cttgtgcgtg tcttcgcgct tcttcatcgc ggcgccgcga
     2821 ccttcggaag catcgatcag ttcgccggca agacgcaggt ccatcgactt ttcaccgcgc
     2881 ttcttggcag cttcgcgcag ccagcgcatg gccagggcca ggcggcgcac gggacgcact
     2941 tcaaccggca cctgatagtt ggcgccaccc acacgacggc tcttcacttc gacgatgggc
     3001 ttgatgttgt tgatggccag agagaagact tccagtggct ctttgccagt cttgctctgg
     3061 acctgctcga acgcaccgta aacgatacgc tcggcgacgg ccttcttgcc gtccaacatc
     3121 acaacgttca tgaacttggc gagttcgacg ctgccgaact tgggatcggg caggatctcg
     3181 cgcttaggga cttcgcggcg acggggcatt tcttgcttcc ttgtgtctgt tcagttgaac
     3241 cgtgggtttc ctcacggccg tggccattta ttcaaatggc cccttacgtt ccgcacgcat
     3301 ccatggacgc agtgcgtttg cacgctcctg acggcatatt gccgcccggg tcttgcgggt
     3361 ttattactgg gctgactgtc cgaacggggc ggatcaggcc ttcttggggc gcttggcacc
     3421 gtacttggag cgcgactgct tgcggtcttt cacgccttgc aggtcaagcg aaccgcggac
     3481 gatgtggtaa cgcacacccg gcaagtcctt cacacggccg ccgcgcacca gcaccaccga
     3541 gtgctcctgc aggttgtggc cttcgccgcc gatgtacgag atgacctcga aaccgttggt
     3601 cagacgcacc ttggcgacct tgcgcagagc cgagttcggc ttcttggggg tggtggtgta
     3661 gacgcgcgtg caaacgccac gacgctgcgg gcaattttcg agcgcagggc tcttgctctt
     3721 gatgatgctg acttcgcgcg gcttgcgcac gagctggctg atggtaggca tggcctcggg
     3781 aattcctctt ttacgtttta acgtagtacc actggaagta catgaatact ggctttctcc
     3841 cagcgccagg acgatggccc gaaaaggtaa ccgacagtga agagaccggt ttacccccag
     3901 acattcgcca tggcgtaggc agctcacgca gaaccgccca taacgtcgca tacgtaaaag
     3961 agcgggtctc gtcccggctg gatccacggg gccggccttg cgaggtcgaa cctcgagcca
     4021 aacccacata aggcgcaccg ggcggcgaca ttgcggcaaa gggagaccta cccctccacg
     4081 cacaaatgta gcagtgagcc cgaaaggtta gcccagatca ggggatttgt caaagattgc
     4141 ttggggataa cctgtacgcc ccagcccagg tctccatgcg gtttgctggc ctggcctgaa
     4201 gcattttgtt atagctggtc cgacctgtac aaaccgatac gcccccaatt acgatacagt
     4261 ccctatccat ggttccccgg gcgcccggac ggccagcccg tccggaaccg ggctttttta
     4321 catttactgg caccaagcgc attgttacta aagagccgtt gatgtagcct gaagtaacag
     4381 agttgtctat agtacacact cttacaaacc ataaaacatg taaagccttt tatccctatt
     4441 catccggccg cagtaacggg ccacaacgga cgaagcgcac acccatgaag atcgcgtcgc
     4501 tcgaagacga tttgaatcag gcccgccaaa ttcagcaggt gctggcctcc gccggctacg
     4561 gcagcgacag tttccagcaa agcgtggacc tgctgagcgc catgcgcagc accacctacg
     4621 acctggtgct gctcgactgg cagttgcccg atatgaacgg cgacgaagta ctgcgccaac
     4681 tgcgcggcca cttcggctac gccattcccg tcatcctgct caccagccgc agccaggaag
     4741 atgacctcgt gcagggcctg caggccggcg ccgacgactt catctccaag cccctgcgtc
     4801 cggccgagct gttggcccgc atcagcgccc tgctgcgtcg ctcgcaaccc gcccaatcgg
     4861 cgcagtcggc cttcacggtg ggcgcctatc acatcgaccc ggtcgagcgc ctcatcaagc
     4921 tcgacagccg caacatcgtg ctggccccca aggagttcga cctggcggtg ctgttctttc
     4981 gcaacatcgg ccggctgctg tcgcgcgacg tgctcgccga aagcgtctgg aaccgcgaga
     5041 ttcccgccac gtcccgcacg ctcgacacgc atctgtccaa catccgccag aagctccagt
     5101 tgcgcgcgca gcacggcgtg cgcctgagct cttcgtacgc cctgggctat cgtctcgacc
     5161 tggtgcaggt ggccgagcac gaaaccacca gcgaccgcgt ctccccattc cccatcgtgg
     5221 tctgagtcca gaccgccgaa cgccgtcctg ccatggtcga aacccgacaa cgtcgacggc
     5281 gcttcgaatg gtccgcggac tggctgctga tatggctgct ggtggccggc gccgccctgc
     5341 tgggcgcgct caacggcctg ggccgcttcg accagggcct ctacgaccag gccatggccg
     5401 catcgagccg gccggcctcg cccgacatcc tcctcgtcgc cgtcgacgac gaatcgctgg
     5461 ccgcgctggg cccctggccc tggccgcgca gcctgcacgc gcaactgctg gatcgcctgc
     5521 acgatgcgcg cgccgtgggc ctggccgtgc tctacagcga gttcgacgcg tccaacccgc
     5581 aagacaatgc cctgctggcg gaggccatca agcggcatgg ccgcgtggtg ctgcccgcca
     5641 cgctcaacca tctgcccacg ccgacccgct acgacaccgc cgacagcacg ctggccgagg
     5701 ccgcggcggg cacggggttt ctcaataccc tgcccgacga cgacggcatc atccgccgcg
     5761 tgcaatggcg cgccaagctg gacaaccgca cctgcctgca catggccctg gccatcctcg
     5821 ccgcgggcgg cgaacaggac aaggtcgacg tcatgctgcg gcaggccggc cccaccggcc
     5881 agataggcat cgcctacgcc gggccgccgg ggcgcctgcg caccgtgtcg tacctgcagg
     5941 tgctccgcgg ccaggtgccg gcggactgga tacgcggcaa gtatgtgctg gtgggttcgc
     6001 aagccagcgg gctgagccgc atgttcccca ccccggtatc gcaccagttc aacggcatga
     6061 gcggcgccga actgctgggc gaactgctgc aggccgcccg cgacgacgtc atgatccgca
     6121 cggcgccggc ctggttcacc acgctgtaca ccgcgctgtc cgtcctgctg ctgtgcctgg
     6181 ccttgcggca catggcgccg cgccgcgcgc tgctggcctc gatcgccgcg gtggccctgg
     6241 tgctggccat atcgttcctg gccatgaact atctgcacta ctgggtggcg cccgcggcca
     6301 cgctcttcat gctggtgctg tgctaccccc tgtggtgctg gcgccagcag gaagccatac
     6361 tgggccacat ggacagcgag ctgcagcgcc tgcgccagga atacccgccc atcctcagcg
     6421 aggcgcagct gccgtcgcgc ctgggcaaga gcgccatgga attgcgcctg cgcgagctgc
     6481 accaggcatt gacgcgcgtg cgcaacctgc ggcgcttcct caccgacggc ttcgatggca
     6541 tcgccgaccc gaccttcgtc tccgacaagg acgggcacct gcgcctgcgc aaccgcgcgg
     6601 cgctggccta tttccgcgat ctttcgctgc gcgcgccgcg cctgggccaa tccacggcct
     6661 ggctgctgga acagctggta tccgatcccg ccgtgctgga cagggtcggc ccggccctca
     6721 ccggccatca gcccgacacc tcgccctggc gcctcgacat ggagatccag gatcgccagg
     6781 gccgcagcat cattctcaag ggcgcgccca tccataccag caccggcgag ttcgccggca
     6841 cggtggtcac tttcaacgac atcagcgcca tccgccaggc cgagcgcaag cgcgaagaaa
     6901 ccctgcgctt cgtctcgcac gacatgcgcg ccccgcaaaa ctcgatcctg gcgctggtct
     6961 acctgaacag cgacgagacc gatccgcaaa agcagcgcga tgcctggtcg cgcatcggcc
     7021 agctggcgcg gcgcaccctg cggctggtcg acgacttcgt gcacctgacc cgcgccgaat
     7081 cggtgcgcat cacgcacgcc gtcatcgacc tgggcatgct ggtgcacgag gcgctggatg
     7141 atttctgggc actggcccag acgcgccggg tggacctgtc catcgacggc gacctgaccg
     7201 aagccctgat ctgcggcgac cgcgccctgc tgctgcgcgc cgtctgcaac ctgatcgaca
     7261 acgcgctcaa gtacacgccc agcggcgggc gcgtgcacgt gtccctgcag cgcgacgggt
     7321 cgagctggct ggtccgcatc gtcgacagcg gcaccggcat cgcggcggaa gacatcccgc
     7381 agatcttcca gccgttctcg cgcgtgggtc cgaccgcgcg caacgacaac aacggcgccg
     7441 gcctgggcct ggccttcgtc aacaccgtgg cctggcgcca tggcggcagc gtcggcgtga
     7501 acagcctgcc cggcgccggt tcgaccttca ccctgcagct gccggcctat acggaagaag
     7561 aagccgcgct ggccaccgaa cgcgccgcct gagctttcgc tcgcggcagg cataaaaaaa
     7621 ccgccggatc cggcggtttg ttgttgggcg cgacatcccg gccgaaaccg ggatgtctgc
     7681 cgcggcttac tcttcgctgg acgccacgtc cgtatcggac tcggcaaccg ccgccgtggc
     7741 cggagccggg gcttcctcga aggggttggc cagctggcgg gcggctt
//
LOCUS       DASWPO010000105         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2790903, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000105 DASWPO010000000
VERSION     DASWPO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790903"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /gene="infC"
                     /locus_tag="VGC69_06210"
     CDS             complement(<1..206)
                     /gene="infC"
                     /locus_tag="VGC69_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY0294913.1"
                     /translation="MATEKSVRINGEIRVPEVRLIGLEGEQLGIVKIADAFRLSEQSD
                     VDLVEIAPNAEPPVCRLMDYGKFK"
     gene            complement(248..2212)
                     /gene="thrS"
                     /locus_tag="VGC69_06215"
     CDS             complement(248..2212)
                     /gene="thrS"
                     /locus_tag="VGC69_06215"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY0294914.1"
                     /translation="MVQITLPDGSQRLYPGPVTVSEVAQSIGTGLAKAALAGRVAADG
                     KEPTMVDTSYRIEQDATLAIVTAKDADGLDLIRHSTAHLLAYAVKELFPDAQVTIGPV
                     IENGFYYDFSYKRPFTPEDLEAIEKKMAELARKDEAVTREEWKRDDAVTFFKSIGEDY
                     KAEIIASIPADQPISLYREGQFIDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSNNEM
                     LQRIYGTAWATKEEQDAYLHMLEEAERRDHRKIGRDLDLFHFQDEGPGLIFWHPKGWA
                     LWQQVEQYMRKVYQDNGYQEVKAPQILDLSLWKKTGHWDNYRENMFTTESENRMYGLK
                     PMNCPGHVQIFNAGLHSYRELPLRYGEFGQCHRNEPSGSLHGMMRVRGFTQDDGHIFC
                     TEDQMLDECTAYTTLLQKVYKDFGFTEVLYKVATRPEKRIGSDEIWDKAENALMESLR
                     RSGCEFVVAPGDGAFYGPKIEYTLKDAIGRHWQCGTMQVDFSMPVRLGAEYVDQSDQR
                     RPPVMLHRAILGSLERFIGMLLEHYAGAMPPWLAPVQAVVCCISEPSADYAAQIVQTL
                     KKQGFRVEADLRGEKITRKIREHSLQKVPYILVVGEKEKQNGMVAVRGRGGKDGGLDL
                     GVMALDEFANRLSEDVATRRSLPQPALAEA"
     gene            2552..3274
                     /locus_tag="VGC69_06220"
     CDS             2552..3274
                     /locus_tag="VGC69_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0294915.1"
                     /translation="MPPFNLPPFACPARAAAAPAEQPRPAARPRLRIAILDDHPITAI
                     GLAAYLGQCAEFDIVLIETSANRFLHGLAQRCCDVAIVDYDLPDAPCDGMSLIRRLRR
                     HDSRMAIITLSADPARDTAYPAFRAGARGYLSKADPVHLIPKLIHAAMAQPSQFHLNI
                     DGQIRPDTPQASATRLSAAETEILRHISLGLSVTQAAERLSRSKKTISTHKRSAMRKL
                     GLADDLALALYLKERFESRLPG"
BASE COUNT          591 a         1109 c         1026 g          671 t
ORIGIN      
        1 tacttgaact tgccgtaatc catcaggcgg cagacaggcg gctcggcgtt gggcgcgatt
       61 tccaccagat ccacgtcgct ttgctcggaa agccggaaag catcggcgat cttgacgata
      121 ccgagctgct ctccttccag acctatcaga cgcacctcgg ggacgcggat ttcaccgttg
      181 atgcgtacgc ttttttcagt ggcgatgttg aaaactccta gtaattactc gaatgcacag
      241 ccggggatca ggcctcggcc agtgcgggtt ggggcaagct gcggcgcgtg gcgacgtctt
      301 cggagaggcg gttggcgaac tcgtcgaggg ccatgacgcc cagatccagg ccgccgtctt
      361 tgccgcccct gcctcgcacg gcaaccatgc cgttttgctt ttccttctcg cctacgacga
      421 ggatgtacgg aaccttttgc aggctgtgct ccctgatttt acgggtgatt ttttcaccgc
      481 gcaaatccgc ctctacccta aagccttgtt ttttcagggt ttgcacaatc tgggcggcat
      541 aatcggccga gggctcggaa atacagcaga ccacggcctg gaccggcgcc agccagggcg
      601 gcatcgcgcc ggcatagtgc tcgagcagca tgccgatgaa acgctccagc gaacccagga
      661 tggcccggtg cagcatcacc ggcgggcggc gctggtcgct ctggtcgacg tactcggcgc
      721 ccagccgcac cggcatggag aaatccacct gcatggtgcc gcactgccag tggcggccga
      781 tggcatcctt cagcgtgtat tcgatcttgg ggccgtagaa ggcgccgtcg cccggcgcca
      841 ccacgaactc gcagcccgag cggcgcaggc tttccatcag cgcgttctcg gccttgtccc
      901 agatctcgtc gctgccgatg cgcttctcgg ggcgcgtggc caccttgtag agcacctcgg
      961 tgaaaccgaa atccttgtat accttctgca gcagcgtggt gtaggccgtg cactcgtcta
     1021 gcatctggtc ttcggtgcag aagatgtggc cgtcgtcctg cgtaaagccg cgcacgcgca
     1081 tcatgccgtg cagcgagccc gagggctcgt tgcgatggca ctggccgaat tcgccgtagc
     1141 gcagcggcag ctcgcggtac gagtgcaggc cggcgttgaa aatctgcacg tggcccggac
     1201 agttcatggg cttcaagcca tacatgcggt tctcggactc ggtcgtgaac atgttttcac
     1261 ggtagttgtc ccagtggccg gtcttcttcc agagcgacag gtcgaggatc tgcggcgcct
     1321 tgacttcctg gtagccgttg tcctggtata ccttgcgcat gtactgctcg acctgctgcc
     1381 acagggccca gcccttggga tgccagaaga tcaggcccgg accttcgtcc tggaagtgga
     1441 acaggtcgag atcgcggccg atcttgcggt ggtcgcggcg ctcggcctct tcgagcatgt
     1501 gcaggtaggc gtcctgctct tccttggtcg cccaggccgt gccgtagatg cgctgcagca
     1561 tctcgttgtt gctgtcgccg cgccaatagg cgccggccac cttcatcagc ttgaacacct
     1621 tgagcttgcc ggtcgacggc acgtgcgggc cgcggcacag atcgatgaac tggccttcgc
     1681 ggtacaggct gatgggttga tccgccggga tcgaggcgat gatctcggcc ttgtagtcct
     1741 cgccgatgct tttgaaaaag gtcaccgcgt cgtcgcgctt ccattcctcg cgcgtgacgg
     1801 cctcgtcctt cctggcgagt tcggccattt ttttctcgat ggcttccagg tcttcgggcg
     1861 tgaaggggcg cttgtacgaa aaatcgtagt agaagccgtt ctcgatcacc gggccgatgg
     1921 tgacctgggc atcggggaac agctccttga cggcataggc cagcaggtga gccgtggagt
     1981 ggcggatgag atccaggccg tcggcatcct tggcggtgac gatggcgagc gttgcgtcct
     2041 gctcgatgcg atagctggtg tcgaccatcg tgggctcttt gccgtccgcc gcgacacggc
     2101 cggccagcgc cgccttggcc aggccggtgc cgatggattg cgcgacttcc gaaaccgtga
     2161 cgggaccggg atacaggcgc tgagaaccat cgggcagagt gatttgtacc atcgaaataa
     2221 tttccagaaa cgacgaacgc ggctggtcgc cgcgttcgtt ttgattgcca tgccggatta
     2281 gccggggcgc cctgcgcgcg gcccaggccg tgccgcaaat acgccggaag aacaacgact
     2341 gcgcctatgg cgcaccaggg cttgattgta gcgctttatg aaaggcaagg gcccctataa
     2401 acagcctcca tcggccctta ttccacccat ttccccctgc aagacgtgcg atcggcggcc
     2461 agaccgtaaa tccgtcccat atatccagtc cacaagacag gaaaatgaaa ggatgacgta
     2521 acatcgagtt actgaagttc ctggccccgc tatgccgcct ttcaacctcc cgccctttgc
     2581 ctgccccgcc cgggctgccg cagcgccagc agagcagccc cgccccgccg ccaggccgcg
     2641 cctgcgcatt gccatcctcg acgaccatcc catcaccgcg atagggctgg ccgcctacct
     2701 gggccagtgc gcggaatttg acatcgtact gatcgagacc tcggccaacc gcttcctcca
     2761 cggcctggcg cagcgatgct gcgacgtggc catcgtggac tacgacctgc ccgatgcccc
     2821 ctgcgacggc atgagcctga tccgccgcct gcggcgccac gacagccgga tggccatcat
     2881 cacgctttcc gccgaccccg cccgcgacac ggcctatccg gcttttcgcg cgggggcccg
     2941 cggctacctg tccaaggccg acccggtgca cctgatcccc aagctgatcc acgccgccat
     3001 ggcgcaaccg agccagttcc acctcaatat cgacggccag atccgcccag acacgccgca
     3061 ggcctcagcc acgcggctga gcgccgccga aaccgagatc ctgcgccata tctccctggg
     3121 cctatcggtc acgcaggcgg ccgaacggct atcgcgcagc aagaaaacca tcagcaccca
     3181 caagcgcagc gccatgcgca agctcggcct ggccgacgac ctggccctgg cgctctatct
     3241 caaggaaaga ttcgaatcgc ggctgccggg gtgacggccc tttcccgccc cgggatcact
     3301 tgtggagttg ccgtaattgc gtcataattt tgcctctgtc ttgctcgtta gctttttacg
     3361 acggcagtgc ccaggtggcg gaattggtag acgcgca
//
LOCUS       DASWPO010000106         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_648621, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000106 DASWPO010000000
VERSION     DASWPO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648621"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..782)
                     /gene="sodB"
                     /locus_tag="VGC69_06225"
     CDS             complement(204..782)
                     /gene="sodB"
                     /locus_tag="VGC69_06225"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEY0294916.1"
                     /translation="MAHTLPPLPYALDALAPYISKETLEFHYGKHHQTYVTNLNNLIP
                     GTEFENLPLEEIVKKSSGGVFNNAAQVWNHTFYWNGLKPQGGGAPAGKLADAINAKWG
                     SFDAFKDAFTKSAVGNFGSGWTWLVKKADGSLDIVNTSNAATPLTTADVPLLTCDVWE
                     HAYYIDTRNARPKYLENFWALVNWDFAAKNLG"
     gene            complement(917..2278)
                     /gene="xseA"
                     /locus_tag="VGC69_06230"
     CDS             complement(917..2278)
                     /gene="xseA"
                     /locus_tag="VGC69_06230"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY0294917.1"
                     /translation="MTIGLDVIDDVFTREILTVAQLNQAVGRLLEQRVPVLWVRGEVS
                     NFTQAASGHWYFTLKDSRAAVRAVMFRSRAGAVGFVPRPGDQVEVRARVSLYEARGDF
                     QLQADGMRRAGLGNLYEAFLRLKEQLAGEGLFDAERKREPVRLPRAIGVVTSLHAAAL
                     RDVLSALARRAPQVPVIIYPAPVQGADAAGKLAAQVAAANARGEVDTLLLVRGGGSIE
                     DLWSFNDEQLARQVAASRIPVISGVGHETDFTIVDFVADVRAPTPTAAAELACAPRAE
                     LLRAAVHAAQGLARAQRRRLDQAAQRLDRVAAQLVSPAQHLAQQRERLATLRHRLAAA
                     WRAPQQGRAARTALLAQSLMHRAPDMGRARDRVQGLAGRLARAQSRRLESRGARLAAA
                     GAQLRAFDPQHTLARGYAIVRDPGGAIVRDAGQLAAGQRLALAFARGEALADVVQVRE
                     VDE"
     gene            2646..3260
                     /locus_tag="VGC69_06235"
     CDS             2646..3260
                     /locus_tag="VGC69_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0294918.1"
                     /translation="MLVLIEAAGWPIWPLLTTSVLGLALIVERLLALRRSLVLPRGLT
                     EQVGDLLRNRQDTPEALAQLERNSPLGRVLAEVLRQRDLPRAELRAAVEDTGRAVAHD
                     LSRYVPAIGTIAAIAPLMGLFGTVVGMIQIFSAYSPGTSDPAQLAHGISVALYNTGLG
                     ILIAIPAMIAHRYLRSRVDGYLSALEQAAARLARHLAAATEPRA"
     gene            3257..3670
                     /locus_tag="VGC69_06240"
     CDS             3257..3670
                     /locus_tag="VGC69_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0294919.1"
                     /translation="MNFRGADHGRDEVEINLIPLIDVLLVMLIFLAATTSFTHFTRLQ
                     ITLPEAAAERQESAPLEIAISQDGHYALNGSLLDAGTTADIAQALIQAAQGKTAPLLV
                     INADAQATHQSVVNVMEAARQAGIERVNFAAQTPQ"
     gene            3667..>4654
                     /gene="lpxK"
                     /locus_tag="VGC69_06245"
     CDS             3667..>4654
                     /gene="lpxK"
                     /locus_tag="VGC69_06245"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY0294920.1"
                     /translation="MSARAYLQRQWQQDGWLPRLLAPLGWLAARAVQRKRAAYRDGRK
                     TAWRAPVPVVVVGNLYVGGTGKTPVVIAAVQGLRALGRTPGVVSRGYGARIGGTPRVG
                     QGDALDPAEYGDEPALIARATAAPVAVHPQRVLAAQALLRAHPGIDVIIADDGLQHLQ
                     LARDVEIVVQDERGLGNGRLLPAGPLREPAARLDEVDAVITNLGSRPGTATAASPAAG
                     AGPLRLAMRLEPGPARRLTDGQTRPLADFAAGPPVAAAAGIGNPQRFFDTLRAAGIAP
                     AEQLALPDHYAYDTSPFGAVQAGRILVTAKDAVKCRRLNDPRLWEVGVTARFT"
BASE COUNT          685 a         1690 c         1577 g          702 t
ORIGIN      
        1 ctgcgcacct ggcggcgcac ttggcccagc aaggtggacg gcggcgaagg gtcgggattt
       61 ttcataggcc tggcatcggc tcgggaagga gaatcaaaaa gaggatcgaa aaggctcaaa
      121 agcacgaggc cccgcgtctc gcgacaggcg gggcctcggg atgcccgcga gacagagctt
      181 gcgggcgcgc ggtacggctg cgatcagccg agattcttgg cggcgaagtc ccagttgacc
      241 agggcccaga agttttccag gtacttggga cgggcgttgc gggtgtcgat gtagtaggcg
      301 tgctcccaga catcgcaggt cagcaggggc acgtcggccg tggtgagcgg cgtggcggcg
      361 ttgctggtgt tgacgatgtc gagcgagccg tcggccttct tgaccagcca ggtccagccc
      421 gaaccgaagt tgcccacggc cgacttggtg aaggcgtcct tgaaggcgtc gaacgagccc
      481 cacttggcgt tgatggcgtc agccagcttg ccggcgggag cgccgccgcc ctggggcttg
      541 aggccattcc agtagaaggt gtggttccac acctgcgcgg cattgttgaa cacgccgccc
      601 gaggacttct tgacgatctc ttccagcggc aggttttcga actcggtgcc ggggatcagg
      661 ttgttcaggt tggtgacgta cgtctgatgg tgcttgccgt agtggaattc gagcgtttct
      721 ttcgagatat acggagccag cgcgtccagc gcgtaaggca ggggaggcaa agtgtgggcc
      781 atgtcgtttc ctttgttatc cgtgcggggt gtgtccgcga tcaagccgat ctattgtatc
      841 ggctagaccg cttctgctgc gactgaattt ccctgggccc gggaaattca ccgatcgcgc
      901 cgcgaaccac tcgggatcac tcgtcaactt cgcgcacctg caccacatcg gccagggctt
      961 cgccgcgcgc gaaggccagc gccaggcgct ggccggccgc cagttgcccg gcatcgcgca
     1021 cgatggcgcc gccgggatcg cgcacgatgg cataaccgcg cgcgagcgtg tgctgcggat
     1081 cgaaggcgcg cagttgcgcg cccgccgccg ccaggcgcgc gccgcgcgat tcgagccggc
     1141 gcgactgggc gcgggccagc ctgccggcca ggccttgtac gcggtcgcgg gcgcggccca
     1201 tgtcgggcgc gcggtgcatc aggctttggg ccagcagggc cgtgcgggcc gcgcggccct
     1261 gttgcggggc ccgccaggcc gccgccaggc ggtgccgcaa agtggccagc cgctcgcgct
     1321 gctgggccag gtgctgcgcc ggcgatacca gttgcgccgc cacgcggtcc agccgctggg
     1381 cggcctggtc gagcctgcgg cgctgcgcgc gggccagccc ctgggccgca tggaccgcgg
     1441 cgcgcagcag ttcggcgcgc ggcgcgcagg cgagctccgc cgccgccgtg ggcgtgggcg
     1501 cccgcacgtc ggccacgaag tcgacgatgg tgaagtcggt ctcgtgcccc accccgctga
     1561 tcacgggaat gcggctggcc gccacctggc gggccagttg ctcgtcgttg aagctccaga
     1621 gatcttcgat gctgcccccg ccgcgcacca gcagcagcgt gtcgacctcg ccgcgcgcat
     1681 tggcggccgc cacctgcgct gccagcttgc ccgcggcgtc ggcgccctgc acgggggcgg
     1741 gatagatgat caccggcacc tggggggcgc gccgggccag cgccgacagc acgtcgcgca
     1801 gggccgcggc atgcagcgag gtcaccacgc cgatggcgcg cggcaggcgc acgggttcgc
     1861 gcttgcgctc ggcgtcgaac aggccttcgc cggccagttg ctccttcagg cgcaggaagg
     1921 cctcgtacag attgcccaga ccggcccggc gcatgccgtc ggcctgcaac tggaaatcgc
     1981 cccgggcctc gtagagcgac acccgcgccc ggacctcgac ctgatcgccc ggacggggca
     2041 cgaagcccac cgcgccggcg cggctgcgga acatgaccgc ccgcacggcg gcccggctgt
     2101 ccttgagggt gaagtaccaa tgcccggagg cggcctgcgt gaaattggac acctcgcccc
     2161 ggacccagag gaccgggaca cgctgctcca gcaaccggcc tacagcctgg ttcaactggg
     2221 cgactgtgag gatttcccgc gtaaatacgt catctatgac gtcaagtcca attgtcataa
     2281 ggctttccgg ggaggtatat gtccacaggg cgcttgtagc gagggttcag gattgtcaag
     2341 ctctccgcct tctgccgcgg ctccctggag agcaagcatt ttccgtgcaa catattgatt
     2401 tatataaata aaaaagatag aaccaattga gctgtttcag tcgattccag ctggaaaccg
     2461 cgcagaaacc ggctcgcggc caagttctac acagaattat ccacaatttc tgtggatagt
     2521 tttgggggct gcggcttgcc tcgcccccgg ccgccgatta cagtctcgcc ctggggcccg
     2581 gcccgccgca aatcgtccga cccgcggccc cagactcccc cctgttgcac cggagcccat
     2641 ccgttttgtt agtcctcatt gaagccgccg gctggccaat ctggccgctg ctcaccacct
     2701 ccgtcctggg gttggccctg atcgtggaac gactcctggc cctgcgccgc agcctggtgc
     2761 tgccccgcgg gctgaccgaa caggtcggcg acctgctgcg caaccgccag gatacccccg
     2821 aggccctggc ccagctcgag cgcaactcgc cgctgggccg cgtgctggcc gaggtgctgc
     2881 gccagcgcga cctgccccgc gccgaactgc gcgccgccgt ggaagacacc ggccgcgccg
     2941 tcgcccacga cctgagccgc tacgtgcccg ccatcggcac catcgccgcg atcgcgcccc
     3001 tgatgggcct gttcggcacc gtggtgggca tgatccagat cttcagcgcc tattcgcccg
     3061 gcaccagcga cccggcccaa ctggcgcacg gcatctcggt ggccctctac aacaccgggc
     3121 tcggcattct catcgccatc ccggccatga tcgcccaccg ctacctgcgc tcgcgcgtcg
     3181 acggctacct gagcgcgctg gagcaggccg ccgccaggtt ggcccgccac ctggccgccg
     3241 ccacggagcc gcgcgcatga atttccgggg cgccgaccac ggccgcgacg aggtcgagat
     3301 caacctgatc ccgctcatcg acgtgctgct ggtcatgctg atcttcctgg cggccaccac
     3361 ctcgttcacc cacttcaccc ggctccagat caccctgccc gaggccgccg ccgagcggca
     3421 ggagtccgcc ccgctcgaga tcgccatcag ccaggacggt cactacgcgc tcaatggctc
     3481 gctgctcgat gccggcacca cggccgacat cgcccaggcg ctcatccagg ccgcgcaagg
     3541 caagactgcc cccctgctgg tgatcaacgc cgacgcccag gccacgcacc agtccgtcgt
     3601 caacgtgatg gaagcggcgc ggcaggccgg catcgaacgc gtcaacttcg ccgcccagac
     3661 ccctcagtga gcgcccgcgc ctacctgcag cggcagtggc aacaagacgg ctggctgccc
     3721 cgcctgctgg ccccgctggg gtggctggcg gcgcgggccg tgcaacgcaa gcgcgcggcc
     3781 tatcgcgacg gccgcaagac cgcctggcgc gcgccggtgc ccgtggtggt ggtcggcaac
     3841 ctctatgtcg gcggcacggg caagacgccg gtcgtgatcg ccgccgtgca gggcctgcgc
     3901 gccctgggcc gcacgcccgg ggtcgtcagc cgcggctacg gcgcgcgcat cggcggcacg
     3961 ccgcgcgtcg gccagggcga tgcgctggac ccggccgagt atggcgacga gccggcgctg
     4021 atcgcccgcg ccaccgccgc ccccgtggcc gtgcacccgc agcgcgtcct ggccgcgcag
     4081 gccctgttgc gcgcccaccc cggcatcgac gtgatcatcg ccgacgacgg cctgcagcac
     4141 ctgcagctgg cgcgcgacgt ggagatcgtc gtgcaggacg agcgcggcct gggcaacggc
     4201 cgcctgctgc ccgccggccc cttgcgcgag ccggccgcgc gcctggacga ggtcgatgcc
     4261 gtgatcacca acctcggttc gcggcccggg accgcgacgg cggcatcgcc cgcggccggc
     4321 gccggcccgc tgcgcctggc catgcgtctc gaacccggcc cggcccgccg cctgaccgac
     4381 gggcagaccc gccccctggc cgacttcgcg gccggcccgc ccgtggcggc ggcggccggc
     4441 atcggcaacc cgcagcgctt tttcgacacg ctgcgggccg cgggcatcgc gcctgccgaa
     4501 cagctcgccc tgcccgacca ctacgcctat gacacctcgc ctttcggcgc cgtccaggcc
     4561 gggcggatcc tcgtcaccgc caaggacgcc gtcaagtgcc ggcgcctgaa cgacccgcgc
     4621 ctgtgggaag tcggcgtgac ggcgcgtttc actc
//
LOCUS       DASWPO010000107        15290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1987041, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000107 DASWPO010000000
VERSION     DASWPO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15290
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987041"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..968
                     /locus_tag="VGC69_06250"
     CDS             117..968
                     /locus_tag="VGC69_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY0294921.1"
                     /translation="MTEQEHTMKHHKRPMAGHQPKTFLWQVSDDHKVATITLNRPDRK
                     NPLTFDSYAELRDLFRALVYATDIKVIVVTGAGGNFCSGGDVHEIIGPLTKMSMPELL
                     DFTRMTGDLVKAMRACPQPIIAAVDGVCAGAGAMMALAADMRLGTPAAKTAFLFTRVG
                     LAGADMGACSLLPRMIGQGRASELLYTGRAMNAEEGSHWGFFNALHDCADLAAAAAKL
                     AAQVAAGPTFAHGVTKKLLHQEWNMGLDEAIEAEAEAQAICMQTQDFHRAYHAFVAKQ
                     KPVFEGN"
     gene            972..2141
                     /locus_tag="VGC69_06255"
     CDS             972..2141
                     /locus_tag="VGC69_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0294922.1"
                     /translation="MRDATWLDWPFFDDTHRALAHELDAWCATALAHVDHHDADAACK
                     QLVRAMGSAGWLRYAVPAGPDGAWGGQLPQVDSRSVCILRETLARHEGLADFAFAMQG
                     LGSGAISLMGSDALRLRYLPRVARGEAIAAFALSEPEAGSDVAAMSCEARLEGDHYVL
                     NGEKTWISNAGIADFYCVFVRTGEAPGARGISAFVVDAGTPGFAVSERFDLIAPHPLG
                     RLSFDNCRIPAGQRLGDPGQGFKLAMMTLDIFRASVAAAALGFARRALDEGLDRARSR
                     KMFGQTLADLQLTQAALGDMDTAIDASALLTYRAAWQRDVKGTRTTREAAMAKMTATE
                     SAQAVIDRALQMFGGAGVVSGQIVEKLYREIRALRIYEGATEVQKLIIARELLKT"
     gene            2176..3825
                     /locus_tag="VGC69_06260"
     CDS             2176..3825
                     /locus_tag="VGC69_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0294923.1"
                     /translation="MERSAHVDTFARDNLPPAAEWPELLLDGPDTRYPARFNCAVELV
                     DAMVARGMGERVALRWRRDGKPAAMSYEELRRLTNRIARVLAEDLQLVPGNRVLLRGP
                     NNPMMAATWLAAMKAGLVTVPTMPLLRSKELKQAIDKAEIRAVLCDARLKDEALACLD
                     ENHEHYCAGLERVVLFNDAAPDAIDALAAGKPDDFAVCDTAADDVCLIAFTSGTTGMP
                     KGCMHFHRDVLAMCDLFPRHILKPQADDVFCGTPPLAFTFGLGGLLCFPLRVGASVVL
                     SERLTPDGLLAEIQDFRASIVFTAPTFYRQMAALVGKYDLASLKKSVSAGEALPDATR
                     QLWKQASGIEMIDGIGGTEMIHVFVSSPPDKVRRGAIGQAVPGYIAQVVDDDMNAVPN
                     GTPGRLAIKGPTGCRYLADERQRKFVQQGWNLPGDTFVQDDDGYFFYQARNDDMIISA
                     GYNIAGPEVEDALLRHEAVAECGVVGKPDEERGQLVKAYVVLKPGQAGTPELAARLQE
                     FVKSIIAPYKYPREIEFVKVLPRTETGKLQRYALRQQGGTQ"
     gene            3822..4301
                     /locus_tag="VGC69_06265"
     CDS             3822..4301
                     /locus_tag="VGC69_06265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0294924.1"
                     /translation="MSAAMQDAHETAGAPSYGSEMQVRFRHCDPAGIVFYPRYFEMIN
                     DFVEEWFDKALGHSYHTLHVDMRLGTPTASIQCDFTAPSRWHDVLRQELAVRRVGGSS
                     ISLDVRFVGQDDGRQRLAASLTIVFVDLKTLRPAPPPDALRSRLAAYLAPAAPAGQA"
     gene            4335..4745
                     /locus_tag="VGC69_06270"
     CDS             4335..4745
                     /locus_tag="VGC69_06270"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0294925.1"
                     /translation="MNILQPPDWLPPKGYSNGVEAEMTAGSKLVFVGGQIGWNGQQQF
                     ETDDFAGQVRLALENIVAVLREAGGGPEHMVRMTWYVKDKHEYVASYPAIGKHYREIM
                     GRNFPAMTAVEVAALVEDRAKVEIEVTAVIPPRD"
     gene            complement(4818..6461)
                     /locus_tag="VGC69_06275"
     CDS             complement(4818..6461)
                     /locus_tag="VGC69_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEY0294926.1"
                     /translation="MSDSTPVELVVDDETRVLSAVYQPMEGAPPPTLESLRHAVRNRG
                     WTEAVLADDMVALFLQACADESEPVRLPVGALLDGSFELHVDRNRMSAMLTVEPPRGG
                     AAVTEEQVRAALAAQGMVAGILDDVLRRAVERGTGEAVCIAEGRDPTPGKPASFENLI
                     ESPRDTHAEEIVDEDDSYIRIDYRDMGSLALVKPGDPLMRRAPAVPGTPGFDLTGRVI
                     EPRPVQDTPFADGLTGAAPAEDDPNLLIATISGAPKIMSRGVSVNSLVEIESVNLDTG
                     NIELDGTLRVKGDITMGMKVRVSGDVFVNGTIEAAEVHADGNITVNGGIIGMTQTVSN
                     DGQEQSRAARLSCGGTVKARFISNAEINAGQIVAVEREVRQSIIDAGASVVVGPPGTQ
                     NGVITGGRIRAMKSVQAGSLGSMSSALTEVHVGMDPHAHVKRAALQQKHKDMVDQKEK
                     LEKLVMFLHANPQKNVNGVGDRARETLKKIIADVAALEVEEVELNKALEPLADAYVAT
                     KKRIYSGVTLYIGSKVREFREDRPGCKAMLDKDGEIALN"
     gene            complement(6804..7811)
                     /locus_tag="VGC69_06280"
     CDS             complement(6804..7811)
                     /locus_tag="VGC69_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0294927.1"
                     /translation="MTASTLFSRLRGVVRLLSVAAVIGGASMAPQAASAAPAYPDHPV
                     TIVVPYPPGGGPDILARVLSEAMGRSLGQAVLVENRGGAGGNIGGQYVAHSRPDGYTL
                     LMCAFSCSVAPLIYTPAPFDLQKDFDPIVLAGKVPSVLLVNPGVPAHTLNELLAYAKS
                     HPGKLNAASSGIGSSPHLAIELLRTVTGVSVTHVPYKGSGQVTGDLLSGQVDMYFDNL
                     PPSLPNIKNGQLRALAVTSEKRSPSLPDVPTFTESGFPDFVLTPWFGLMAPANTPDAV
                     LDKLNQAVNLALKDPTVAQRVKDLGVEDAGGTRADITAFINGETKKWQRVVKQNHVQA
                     Q"
     gene            complement(7937..9073)
                     /locus_tag="VGC69_06285"
     CDS             complement(7937..9073)
                     /locus_tag="VGC69_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0294928.1"
                     /translation="MKITAIETIELDAPLEKPWKIATFTLSSLTACLVRIRTDEGIFG
                     IGEAIARFGPGVTAAVVHEILAPVLLGKDPRNIEGLWDAMARTMRPRGHSRGFHLEAM
                     AGVDIALWDLLGQAAGKPVWQLLAGHGRGNIPVYASSILLDTPERMANEARKLVTAGY
                     AAIKVKVGQSARVDAERIEAIRAEVGSGVDLMLDCNSGFEPTDAIQFGRTAERLGIYW
                     MEEPVNPDDLQGYRRVRSALKDVRIAAGEGEFTAGGFVPFLQEGLLDVVQPDIARCSG
                     FTGARRVAALANAFNAAVAPHTGASGPVCIAATMHLSAAIPNFLVFEDMYIHNPLHEV
                     LSVPLPTQAASQIAVPTGPGLGISLNEDALSRFSRQGWRASSLS"
     gene            9196..9867
                     /locus_tag="VGC69_06290"
     CDS             9196..9867
                     /locus_tag="VGC69_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY0294929.1"
                     /translation="MVRSTKLPDQVYELLAHKISVGEFSAGERLPSETQLASEFGVSR
                     TVLREAIARLKNEGLVTTRHGLGMFVADSFDAVPFRVSALTRADSRELFELRMGFETE
                     AAALAAKRRTVEQLRTLAQAIEAMEGAVQAGEDGVQEDFQFHRALAEATNNGMYRSFL
                     AFLERHIHRQLLVSRKNSHANGKFADVVQEHRAIYKAVEAGDSDAARQAAHNHLQNGL
                     YRLQF"
     gene            complement(9920..11203)
                     /gene="hemL"
                     /locus_tag="VGC69_06295"
     CDS             complement(9920..11203)
                     /gene="hemL"
                     /locus_tag="VGC69_06295"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY0294930.1"
                     /translation="MPTNAELFDRACRSIPGGVNSPVRAFRSVGGTPRFIQRAAGPHV
                     WDAEGTRYIDYVGSWGPAILGHAHPEVVRAVQDAAVGGLSFGAPTEAEVELAELLIAR
                     LPSMEQVRLVSSGTEATMTAIRLARGATGRAKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPPEFVAHTLTLEYNNLQAVQAAFDQHGAEIACVIVEPAAGNMNLIMPRPG
                     FLQGLRELCTRHGAVLVFDEVMTGFRVGPQGMQGLAGIRPDLTTLAKVIGGGMPVGAF
                     GGRADLMAHIAPLGGVYQAGTLSGNPVAVAAGLATLRLVGQPGFYEDLSERTARLADG
                     LQARARAASLAFSAQSVGGMFGLYFSAQVPETFAEVSACDTGLFKRFFHAMLDRGVYF
                     APSAFEAGFVSAAHGDATLQATFDAAEQAFATLAP"
     gene            complement(11226..11897)
                     /gene="thiE"
                     /locus_tag="VGC69_06300"
     CDS             complement(11226..11897)
                     /gene="thiE"
                     /locus_tag="VGC69_06300"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0294931.1"
                     /translation="MTALRFPRGLYGVTPEWDDTGKLLHAVRQAAAGGMRALQLRRKH
                     ISEAERAAQARELAPLCRALGVVFLINDHWRLALEVGADGVHLGRDDADPAQVRKVAG
                     PGLLIGVSCYNELDRARRLLAAEPDYIAFGAVFTSPTKPQAVHAPLPLLAQARELAAG
                     CGLPRPAVAAIGGITPDNAALAAQAGADAIAVITGLFEAPDIAAAARACAAPFQAAPT
                     PGPQA"
     gene            complement(11935..12750)
                     /locus_tag="VGC69_06305"
     CDS             complement(11935..12750)
                     /locus_tag="VGC69_06305"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY0294932.1"
                     /translation="MISAVHPPLTLVFGPVDPTGADGLPADAVTCASLGCHCLAAVTA
                     LTVQDTAGIEEIHPVSPELLDDQARCLLEDMPVQAIKVGGLYSAETASVAAQIAADYS
                     QVPLVLHLGHRSVASDDAAQEDDADDLLAATFELLLPQAQLVVVEHLRLAQWQADGSI
                     DMSESPSPAHALLAGGAQWALVLGAPMRPGHQENILLGPDGQTHTWPWQAPPERNGDT
                     GGVVAAAATALLAQGLDMPRAAELALQHAAQALSTGFLPGMGRRVPNRVGTPA"
     gene            12895..13059
                     /locus_tag="VGC69_06310"
     CDS             12895..13059
                     /locus_tag="VGC69_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015389172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEY0294933.1"
                     /translation="MRTWMCLICGWVYDEEAGLPEEGIAPGTRWEDVPPNWVCPECGA
                     RKEDFELMEI"
     gene            13196..13720
                     /locus_tag="VGC69_06315"
     CDS             13196..13720
                     /locus_tag="VGC69_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY0294934.1"
                     /translation="MAMPGMGDGSFAGTVIYVCEHTTRGALGLVINRPTDLTLASLFE
                     RIDLQLEIAPARDDIVFFGGPVQTDRGFVLHAPAGQYTSSIRLGELTLTTSRDVLQAV
                     ADGTGPARLLVTLGYAGWGAGQLEYEMSQNAWLSVGADPRIIFDVPPEARYPEALKLL
                     GVDPVMLSGGAGHA"
     gene            13713..14210
                     /gene="ruvX"
                     /locus_tag="VGC69_06320"
     CDS             13713..14210
                     /gene="ruvX"
                     /locus_tag="VGC69_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY0294935.1"
                     /translation="MPDTSPQGGREATLLGFDFGEKKIGVAIGNTLTRQARPLEIIFS
                     ELREARFGRIAQLLAEWQPQCLVVGLALAADGGEQPATARCRRFANQLNGRFGLPVVL
                     VDERGSSLEAQDLLKGMAPAPRPSGLLPPKGGVPALGRPGGRHAADDAMAAMIILQRH
                     LDTLP"
     gene            14284..15027
                     /locus_tag="VGC69_06325"
                     /pseudo
     CDS             14284..15027
                     /locus_tag="VGC69_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
BASE COUNT         2424 a         5199 c         5210 g         2457 t
ORIGIN      
        1 ctgcccccgc ctcaaagggc cggcaggggc ttgcccgccg aacggacgcc tcaagaaccc
       61 agattgaatc gaccgaaatc gaattaactg aaccgaccag caggagacac gacgatatga
      121 ccgaacaaga acacaccatg aagcaccaca agcgtcccat ggccggccac cagcccaaga
      181 ccttcctgtg gcaagtgtcc gacgaccaca aggtcgccac catcacgctg aaccgccccg
      241 accgcaagaa cccgctcacc ttcgactcct acgccgaact gcgcgacctc ttccgcgccc
      301 tggtctatgc caccgacatc aaggtcatcg tcgtcaccgg cgccggcggc aacttctgct
      361 ccggcggcga cgtgcacgaa atcatcggcc cgctcaccaa gatgagcatg cccgaactgc
      421 tcgacttcac ccgcatgacc ggcgacctcg tcaaggccat gcgcgcctgc ccgcagccca
      481 tcatcgcggc ggtggacggc gtgtgcgccg gcgccggcgc catgatggcc ctggcggccg
      541 acatgcgcct gggcacgccc gccgccaaga cggccttcct gttcacccgc gtcggcctgg
      601 ccggcgccga catgggcgcc tgctccctgc tgccgcgcat gatcggccag ggccgcgcca
      661 gcgaactgct ctacaccggc cgtgccatga acgccgaaga aggcagccac tggggcttct
      721 tcaacgccct gcacgactgc gccgacctgg ccgccgccgc cgccaagctg gccgcccagg
      781 tcgccgccgg ccccaccttc gcccacggcg tcaccaagaa actgctgcac caggaatgga
      841 atatgggcct ggacgaggcc atcgaagccg aagccgaggc ccaggccatc tgcatgcaga
      901 cccaggactt ccaccgcgcc taccacgcct tcgtggccaa gcaaaaaccc gtttttgaag
      961 gaaactgagc catgcgcgat gcgacctggc tggactggcc cttcttcgac gatacccacc
     1021 gcgcgctggc gcacgaactc gatgcctggt gcgccacggc gctcgcccat gtcgaccacc
     1081 acgacgccga cgccgcctgc aagcaactgg tgcgcgccat gggctccgcc ggctggctgc
     1141 gctatgccgt gccggcgggc cccgacggcg cctggggcgg ccaactgccc caggtcgatt
     1201 cgcgctcggt ctgcatcctg cgcgaaaccc tggcccggca cgaaggcctg gccgacttcg
     1261 ccttcgccat gcagggcctg ggcagcggcg cgatctcgct gatgggatcc gacgcactgc
     1321 gcctgcgcta cctgccgcgc gtggcgcgcg gcgaggccat cgccgccttc gccctgtccg
     1381 agcccgaggc cggttcggac gtggccgcca tgtcgtgcga agcgcgcctc gaaggcgatc
     1441 attacgtgtt gaacggcgag aaaacctgga tttccaatgc cggtatcgcc gacttctact
     1501 gtgtgttcgt gcgcacgggc gaagcccccg gcgcgcgcgg catcagcgcc ttcgtggtcg
     1561 acgccggcac gcccggcttc gccgtgtccg agcgcttcga cctgatcgcg ccgcacccgc
     1621 tgggccgcct gagcttcgac aactgccgca tccccgccgg ccagcgcctg ggcgatcccg
     1681 gccagggctt caagctggcc atgatgacgc tggacatctt ccgcgcctcg gtggccgccg
     1741 ccgcgctggg cttcgcgcgc cgcgcgctgg acgaaggcct cgaccgcgcc cgcagccgca
     1801 agatgttcgg ccagaccctg gccgacctgc agctcacgca ggccgcgctg ggcgacatgg
     1861 acacggccat cgacgcctcg gcgctgctga cctaccgcgc cgcctggcag cgcgacgtca
     1921 agggcacgcg caccacgcgc gaggccgcca tggccaagat gacggccacc gaatcggccc
     1981 aggccgtgat cgaccgcgcc ctgcagatgt tcggcggcgc cggcgtcgtg tcgggccaga
     2041 tcgtcgagaa gctctaccgc gagatccgcg cgctgcgcat ctacgaaggg gcgaccgagg
     2101 tgcagaagct catcatcgcc cgcgaactgc tcaagacctg agaaacgcgg ccggaatcat
     2161 tacaagggga atcgcatgga aagatccgca cacgtcgata cgtttgcccg ggacaacctg
     2221 ccgccagccg ccgaatggcc cgagctgttg ctcgacggcc cggacacgcg ctatccggcg
     2281 cgcttcaatt gcgcggtcga actggtcgac gccatggtgg cgcgcggcat gggcgagcgc
     2341 gtggcgctgc gctggcgccg cgacggcaag ccggccgcca tgagctacga ggaactgcgc
     2401 cgcctgacca accgcatcgc ccgcgtgctg gccgaggacc tgcaactggt gccgggcaac
     2461 cgcgtgctgc tgcgcggccc caacaacccc atgatggccg cgacctggct ggccgctatg
     2521 aaggccggcc tggtcaccgt gcccaccatg ccgctgctgc gctccaagga gctcaagcag
     2581 gccatcgaca aggcggagat acgcgccgtg ctgtgcgacg cgcggctcaa ggacgaggcg
     2641 ctggcctgcc tcgacgagaa ccacgagcac tattgcgccg gcctggaacg cgtggtgctg
     2701 ttcaacgacg ccgcgcccga cgcgatcgat gctttggcgg cgggcaagcc cgacgacttc
     2761 gctgtgtgcg ataccgcggc cgacgacgtc tgcctgatcg cgttcaccag cggcacgacg
     2821 ggcatgccca agggctgcat gcacttccac cgcgacgtgc tggccatgtg cgacctgttc
     2881 ccgcgccaca tcctcaagcc gcaggccgac gacgtgttct gcggcacgcc gcccctggct
     2941 ttcaccttcg gcctgggcgg gctgctgtgc tttccgctgc gcgtgggcgc cagcgtggtg
     3001 ctgtcggaaa ggctcacgcc cgacggcctg ctggccgaga tccaggattt ccgcgccagc
     3061 atcgtcttca ccgcgcccac cttctaccgc cagatggcgg cgctggtcgg caagtacgac
     3121 ctggcgtcgc tgaagaaaag cgtgtcggcc ggcgaagccc tgcccgacgc cacgcgccag
     3181 ctgtggaagc aggcgagcgg catcgagatg atcgacggca tcggcggcac cgagatgatc
     3241 cacgtgttcg tctccagccc gccggacaag gtgcggcgcg gcgccatcgg ccaggcggtg
     3301 cccggctaca tcgcccaggt ggtcgacgac gacatgaatg ccgtgcccaa cggcacgccc
     3361 ggccgcctgg cgatcaaggg gcccaccggc tgccgctacc tggccgacga gcgccagcgc
     3421 aagttcgtgc agcagggctg gaacctgccc ggcgacactt tcgtgcagga cgacgacggc
     3481 tatttcttct atcaggcgcg caacgacgac atgatcatct cggccggcta caacatcgcc
     3541 ggtcccgagg tggaggacgc cctgctgcgc catgaggccg tggccgagtg cggcgtggtg
     3601 ggcaagcccg acgaggagcg cggccaactg gtcaaggcct atgtggtgct caagccgggc
     3661 caggcaggca cgcccgagct ggcggcccgg ctgcaggaat tcgtcaaatc catcatcgcc
     3721 ccctacaagt atccgcgcga gatcgagttc gtgaaggtgc tgccgcgcac cgagaccggc
     3781 aagctgcagc gctatgccct gcggcagcag ggcgggacgc aatgagcgcg gcgatgcagg
     3841 acgcgcacga aacggcgggc gcgccttctt atggcagcga aatgcaggtg cgctttcgcc
     3901 actgcgaccc ggccggcatc gtgttctatc cgcgctattt cgagatgatc aacgacttcg
     3961 tcgaggagtg gttcgacaag gcgctgggcc attcttacca tacactgcac gtcgacatgc
     4021 gcctgggcac gcccacggcg tccatccagt gcgatttcac ggcgcccagc cgctggcacg
     4081 acgtgctgcg ccaggagctg gcggtgcggc gcgtcggcgg ctcctcgatc tcgctcgacg
     4141 tacgcttcgt gggccaggac gacggccgcc agcgcctggc cgccagcctg accatcgtgt
     4201 tcgtcgactt gaagaccctc cgccccgcgc cgcccccgga cgcgttgcgc tcgcgcctgg
     4261 ccgcctacct tgcgcccgcc gcgccggcgg gccaggcatg atccattccg ctttacctcc
     4321 aacaggatga tgtgatgaac attcttcaac ccccggattg gctgccgccc aagggctatt
     4381 ccaacggcgt cgaagccgaa atgaccgccg gctcgaagct ggtcttcgtc ggcgggcaga
     4441 tcggctggaa tggccagcag caattcgaga ccgacgattt cgccggacag gtgcgcctgg
     4501 cgctggaaaa catcgtcgcc gtgctgcgcg aggcgggcgg cggccccgag cacatggtgc
     4561 gcatgacctg gtacgtgaag gacaagcacg agtatgtggc ctcgtacccc gccatcggca
     4621 agcactaccg cgaaatcatg ggccgcaatt tcccggccat gacggccgtc gaagtggccg
     4681 ccctggtcga ggaccgcgcc aaggtcgaga tcgaagtgac ggccgtgatc ccgccgcgcg
     4741 actgacccca agccgggcaa ggcgccataa aaatgggccc gcgggcccat ttttttattg
     4801 caaccgtcga tcggcgatca gttcagcgcg atttcgccgt ccttgtcgag catggccttg
     4861 cagccgggcc ggtcttcccg gaattcgcgg accttgctgc cgatgtacag cgtcacgccg
     4921 ctgtagatgc gcttcttggt ggccacgtag gcgtcggcca gcggctccag cgccttgttg
     4981 agctcgactt cctcgacttc gagggcggcg acgtcggcga tgatcttctt gagcgtttcg
     5041 cgcgcccggt cgcccacgcc gttgacgttt ttctgcggat tggcgtgcag gaacatgacc
     5101 agcttttcca gcttttcttt ctggtcgacc atgtctttgt gcttttgctg caaggcggcg
     5161 cgcttgacgt gcgcgtgcgg gtccatgccc acgtggactt cggtgagcgc cgacgacatc
     5221 gagccgagcg agcccgcctg caccgatttc atggcccgga tgcgcccgcc ggtgatcacg
     5281 ccgttctgcg tgccgggcgg gccgaccacc acgcttgcgc cggcgtcgat gatgctttgg
     5341 cgcacttcgc gctcgaccgc cacgatctgg cccgcgttga tctcggcgtt gctgatgaag
     5401 cgcgccttga ccgtgccgcc gcagctcagg cgggccgcgc gggattgttc ctggccgtcg
     5461 ttggagacgg tctgcgtcat gccgatgatg ccgccgttga ccgtgatgtt gccgtcggca
     5521 tggacttcgg cggcttcgat ggtgccgttg acgaagacgt cgcccgatac gcgcaccttc
     5581 atgcccatgg tgatgtcgcc cttgacccgc agcgtgccgt cgagctcgat attgccggtg
     5641 tcgaggttca ccgactcgat ctcgaccagc gagttcaccg acaccccgcg cgacatgatc
     5701 ttgggcgcgc cggagatcgt ggcgatgagc aggttgggat cgtcttcggc gggggctgcg
     5761 ccggtgaggc cgtcggcaaa gggcgtgtcc tgcaccggcc gcggttcgat gactctgccg
     5821 gtcaggtcga agccgggcgt gcccggcacg gcgggcgcgc ggcgcatcag cggatcgccc
     5881 ggcttgacca gcgccaggct gcccatgtcg cggtagtcga tccggatgta ggagtcgtct
     5941 tcgtcgacga tctcctcggc gtgggtgtcg cgcggcgact cgatcaggtt ctcgaaggag
     6001 gcgggcttgc cgggggtggg gtcgcggcct tcggcgatgc acacggcctc gccagtgccg
     6061 cgctcgacgg cccggcgcag gacgtcgtcc aggatgccgg ccaccatgcc ttgcgcggcc
     6121 agggcggccc gcacctgctc ttcggtgacg gccgcgccgc cgcgcggcgg ctcgaccgtc
     6181 agcatggcgg acatgcggtt gcggtcgacg tgcagttcga agctgccgtc gagcagcgcg
     6241 cccacgggca gccgcaccgg ctcgctttcg tcggcgcagg cttgcaggaa cagggcgacc
     6301 atgtcgtcgg ccagcacggc ctcggtccag ccgcggttcc tgacggcatg ccgcaggctc
     6361 tccagcgtgg gagggggcgc gccttccatg ggttggtaaa cggcggacag gacgcgcgtt
     6421 tcatcatcca cgaccagttc gaccggcgtc gaatcagaca tgaagcctcc tggagtcgct
     6481 ttggggatag cggccggcac tgacctagac gggcgcgccg gatgttttag taaataattc
     6541 aatagtgaca tagtgctaca tacgttgcag cgccattaaa gcatccaatt gcagacttga
     6601 taacgcaagc cttgcgattc ccccggtcac aaatgtcaaa aaagaaaagg gccgtatcac
     6661 ggccctttgc aacggcaagc agcttggcgg cgctcaggga gcaagcgtgg caaaagcctg
     6721 ttccgcggca tcgaaagtgg cctgcagcgt ctcgtcgccg tgggcggccg acacgaagcc
     6781 ggccgcgatc ccgccgcgcg cggctactgc gcctggacgt gattctgttt gacgacccgt
     6841 tgccatttct tcgtctcgcc gttgatgaat gcggtaatgt ccgctcgcgt gccgccggcg
     6901 tcctccacgc cgagatcctt gacccgctgg gcgaccgtgg ggtccttgag cgcaaggttt
     6961 accgcctggt tcagtttgtc caggacggcg tcgggggtat tggcgggggc catgaggccg
     7021 aaccacggcg tcagcacgaa atcgggaaac ccggactcgg tgaaagtggg cacgtccggc
     7081 agcgacggcg agcgcttttc actcgtcacg gccagggcac gcagttgtcc attcttgatg
     7141 ttgggcagcg agggcggcag gttgtcgaag tacatgtcga cttgtccgct cagcaggtcc
     7201 cccgtcactt ggccagagcc tttgtatgga acgtgggtca cgcttacccc ggtcaccgtg
     7261 cgcagcagtt cgatcgccag gtgcggcgag ctgccgatgc ccgaggaggc cgcattgagc
     7321 ttgcccggat gcgacttggc ataggccagc agttcgttca gcgtatgcgc gggcacgccg
     7381 gggttgacca gcaggacgct gggaaccttg ccggccagca cgatcgggtc gaaatccttc
     7441 tgcagatcga acggcgcagg ggtatagatc agcggggcga cgctgcaact gaacgcgcac
     7501 atgagcagtg tgtatccgtc gggcctggaa tgggcgacgt actgtccgcc gatattcccg
     7561 cccgcgccgc cgcggttctc cactaggacc gcctggccga ggctgcgtcc catggcttca
     7621 ctgaggacgc gggccaggat gtcgggcccc cctcctggcg ggtacggcac cacgatggtg
     7681 accggatgat ccgggtaagc cggcgcggcg gatgccgctt gcggcgccat ggacgcgccg
     7741 ccaatcacgg ccgctacgga caagagtcgg acaacgccgc gcaggcgcga aaacagagtg
     7801 ctagcagtca atttttgtct ccagaaacgc cccgttgcac gaggggcttg tcgaaatggt
     7861 cttcggtcgg gcccgtcttg ccgccggacg gaccctgcat ggagggcgtc cggcacggcg
     7921 gcaggacgcg ccggtattac gagaggctgc tggcgcgcca cccctggcgt gagaaccggg
     7981 acaacgcgtc ttcgttcagg ctgatgccca ggcccggccc ggtcgggacg gcgatctggc
     8041 tggcggcttg cgtcggcagc ggtacggaca gcacctcgtg cagcgggttg tggatgtaca
     8101 tgtcctcgaa caccaggaag ttcggtatcg cggcggacag gtgcatggtg gcggcgatgc
     8161 acactggccc ggacgcgccg gtgtgcggcg ccaccgcggc attgaaggcg ttcgccaggg
     8221 ccgccacccg gcgcgcgcct gtgaagcccg agcaccgcgc gatatcgggc tgcacgacgt
     8281 cgagcaggcc ttcctgcaag aagggcacga aaccgcccgc cgtgaattcg ccttcccccg
     8341 cggcgatccg cacgtccttc agggccgagc gtacccggcg gtagccttgg agatcgtccg
     8401 gattgaccgg ttcttccatc cagtagatgc ccagccgttc ggctgtgcgg ccgaactgga
     8461 tggcatcggt cggctcgaag ccgctgttgc agtcgagcat gaggtcgacg ccagagccca
     8521 cctcggcgcg tatggcctcg atccgctccg cgtcgacgcg ggccgactgc ccgaccttga
     8581 ccttgatggc cgcgtagccg gccgtcacca gtttgcgggc ctcgttcgcc atgcgttcag
     8641 gcgtgtcgag caggatcgac gaggcgtaga cagggatgtt gccgcggccg tggcccgcca
     8701 gaagctgcca gaccggcttg cccgcggctt gccccaatag gtcccatagc gcgatgtcca
     8761 cgccggccat cgcctccagg tgaaagccac gggaatggcc acgcggccgc atggtgcgcg
     8821 ccatggcatc ccagagcccc tcgatgttgc ggggatcctt gcccaggagc acgggcgcga
     8881 ggatctcgtg caccacggcc gcggtcacac ccggtccgaa ccgggcgatg gcctccccga
     8941 tgccgaatat gccttcatca gtccggatac gtaccaggca tgccgtcagc gatgacaggg
     9001 tgaaggtcgc gattttccat ggtttttcca ggggggcgtc gagttcgatg gtttctatgg
     9061 cggtgatttt cactgcgggc tctccatgtc atgccgaagc cattcggcag ttttatttac
     9121 attagatgac ctggtcatca tacaagtaga catcatagca ctgtgtgata cgatgcccga
     9181 atgactgttg gtacgatggt gcgcagcacc aagctccccg atcaggtcta cgagctgcta
     9241 gcccacaaga tttccgtggg cgaattcagc gcgggcgagc ggctgccctc ggaaacccag
     9301 ctggccagcg agttcggcgt cagccgcacg gtcctgcgcg aagccatcgc acgcctgaaa
     9361 aacgaggggt tggtcaccac gcgccacggc ctgggcatgt ttgtcgccga ttcgttcgat
     9421 gcggtgccct tccgggtcag cgctttgacc cgtgccgaca gccgggaact gttcgagctg
     9481 cgcatgggct tcgaaaccga agccgccgcc ctggccgcga aacgacgcac ggtcgagcag
     9541 ctacgcaccc tggcgcaagc gatcgaggcc atggaaggcg cggttcaggc cggggaggat
     9601 ggcgtccagg aggatttcca gttccaccgt gctttggccg aagccaccaa caacggcatg
     9661 taccgcagct ttcttgcctt cctcgaacgg catatccatc ggcagttgct cgtaagccgc
     9721 aagaacagcc atgccaacgg caagttcgcc gatgtcgtgc aggagcatcg ggccatctac
     9781 aaggcagttg aggcgggcga ttccgacgcc gcccgccagg cggcccacaa tcatctccag
     9841 aacggcttgt accggctgca gttctgactc aaaaaggccg catcacggcc ctttgcaacg
     9901 gcaagcagct tggcggcgct cagggagcaa gcgtggcaaa agcctgttcc gcggcatcga
     9961 aagtggcctg cagcgtcgcg tcgccgtggg cggccgacac gaagccggcc tcgaaggccg
    10021 acggggcgaa atacacgccg cgatcgagca tggcgtggaa aaagcgcttg aagaggccgg
    10081 tgtcgcaggc cgagacttcg gcaaaggttt ccggcacttg cgcactgaag tacaggccga
    10141 acatgccgcc gaccgactgc gccgagaagg ccaggctggc tgcgcgggcg cgtgcctgca
    10201 gcccgtcggc cagccgggcc gtgcgctcgc tcaggtcttc gtagaaaccg ggctggccga
    10261 ccagcctgag cgtggccagg cccgcggcca cggccaccgg gttgcccgac agcgtgcctg
    10321 cctggtagac gccgcccagg ggggcgatgt gcgccatcag gtcggcccgg ccgccgaagg
    10381 cgcccaccgg catgccgccg ccgatgacct tggccagggt ggtgaggtcg ggacgtatgc
    10441 cggccaggcc ctgcatgccc tgcgggccga cgcgaaagcc cgtcatcacc tcgtcgaaga
    10501 ccagcacggc gccgtggcgc gtgcacagtt cgcgcaggcc ctgcagaaag cccggccgcg
    10561 gcatgatcag gttcatgttg ccggccgcgg gctcgacgat cacgcaggcg atctcggcgc
    10621 cgtgctggtc gaaggcggcc tgcacggcct gcaggttgtt gtattcgagg gtgagggtgt
    10681 gtgccacgaa ctcgggcggc acaccggccg agctggggtt gccgaaggtg agcaggcccg
    10741 agccggcctt gaccagcagg ctgtcggaat ggccgtggta gcagccttcg aacttgatga
    10801 tcttggcgcg gccggtggcg ccgcgcgcga gccgaatcgc cgtcatggtg gcctcggtgc
    10861 ccgagctcac caggcgcacc tgctccatcg agggcagccg ggcgatcagc agctcggcca
    10921 gttcgacttc ggcctcggtc ggggcgccga aggacaggcc gcccacggcg gcgtcctgca
    10981 cggcgcgcac gacctcgggg tgggcatggc ccaggatggc cgggccccac gagccgacgt
    11041 aatcgatata gcgcgtgcct tcggcatccc agacatgggg gcccgcggcg cgctggatga
    11101 agcgcggcgt gccgccgacc gagcggaagg cgcgcacggg tgaattgacg ccgccgggaa
    11161 tgctgcggca ggcgcggtcg aagagttcag cgttggtggg catgcttgtc gggaggtagg
    11221 aagggtcagg cttgcgggcc gggggtgggg gcggcctgga atggcgcggc gcaggcgcgc
    11281 gcggctgccg cgatgtcggg ggcttcgaac aggcccgtga tgacggcgat ggcatcggcg
    11341 ccggcctggg ccgccagcgc ggcgttgtcc ggcgtgatgc cgccgatggc cgccacggcc
    11401 gggcgcggca ggccgcagcc cgccgccagt tcgcgggcct gcgccagcag cggcagcggc
    11461 gcgtgcacgg cctgcggctt ggtgggcgag gtgaacacgg cgccgaaggc gatgtagtcg
    11521 ggttcggccg cgagcaggcg ccgcgcgcgg tcgagctcgt tgtagcagga cacgccgatg
    11581 agcaggccgg ggccggccac cttgcggacc tgggccgggt cggcatcgtc gcggcccagg
    11641 tgcacgccgt cggcgccgac ctcgagcgca aggcgccagt ggtcgttgat gaggaacacc
    11701 acaccgagcg cgcggcacaa gggcgccagt tcgcgcgcct gcgccgcgcg ttcggcctcg
    11761 gaaatatgct tgcggcgcag ctgcagcgcg cgcatgccgc cggccgcggc ctggcgcacg
    11821 gcgtgcagga gcttgccggt gtcgtcccac tcgggggtca cgccgtacag gccgcgcgga
    11881 aatcgcaatg cagtcatggg taatgggtct ccgtgccgcg tgcgcctgac gggatcaggc
    11941 cggcgtgccg acgcggttgg gaacgcgccg gcccatgccg ggcagaaagc ccgtggacag
    12001 ggcctgggcg gcatgctgca gcgccagttc ggcggcgcgc ggcatgtcca ggccctgggc
    12061 cagcagggcc gtggcggcgg cggccaccac gccgccggtg tcgccgttgc gctcgggcgg
    12121 cgcctgccag ggccaggtgt gggtctggcc gtcggggccg agcaggatgt tttcctggtg
    12181 gccggggcgc atgggcgcgc ccagcaccag ggcccattgc gcgccgccgg ccagcagggc
    12241 gtgcgccgga ctgggggact ccgacatgtc gatgctgccg tcggcctgcc actgcgccag
    12301 gcgcaggtgc tcgaccacca ccagctgcgc ctgcggcagc agcagctcga aggtggcggc
    12361 cagcaggtcg tcggcgtcgt cctcttgcgc ggcatcgtcg ctggccacgc tgcgatggcc
    12421 caggtgcagc accaggggca cctggctgta gtccgcggcg atctgcgccg ccacgctggc
    12481 ggtctcggcg ctgtagaggc cgcccacctt gatggcctgg acgggcatgt cttccagcag
    12541 gcagcgcgcc tggtcgtcga gcagctcggg cgagacgggg tggatctctt cgatgccggc
    12601 cgtgtcctgc acggtgagcg cggtgacggc ggccaggcag tggcagccca ggctggcgca
    12661 ggtcacggcg tcggcgggca ggccatcggc gccggtgggg tcgacaggcc cgaacacgag
    12721 cgtcagaggg ggatggacag cggaaatcac ggatatcgac ggcggctggg ccgcagcagt
    12781 tttgatcggg tttaggtaag atcgtccatt cattctaatt gatacccctg actgccaagt
    12841 gttgcgccct cctgcaatgc ccgcggccgc cacgggcctg gaacagtgag aaccatgcgt
    12901 acctggatgt gtttgatttg cggttgggtg tatgacgagg aagccggcct gcccgaagag
    12961 ggcatcgccc ccggcacgcg ctgggaagac gtgcccccca actgggtttg ccccgagtgc
    13021 ggcgcgcgca aggaagattt cgaattgatg gaaatctgag tgccctgttg ctgttccctc
    13081 tgctcgttcc atccagccgg agagccccat gaccgaagac acgcacgatg acggcgcggc
    13141 ggacgaccag tcgtccgccg cgccgccggc cgcggatttc tccaaccagt tcctgatggc
    13201 catgcccggc atgggcgacg gcagcttcgc cggcacggtg atctatgtct gcgagcacac
    13261 gacgcgcggc gcgctgggcc tggtgatcaa ccggcccacc gacctcacgc tcgcctcgct
    13321 cttcgaacgc atcgacctcc agctcgagat cgccccggcg cgcgacgaca tcgtgttctt
    13381 cggcggcccc gtgcagactg accgcggctt cgtgctgcac gccccggccg ggcaatacac
    13441 ctccagcatc cggctgggcg agctcacgct caccacctcg cgcgacgtcc tgcaggccgt
    13501 ggccgacggc accggcccgg cgcgcctgct ggtgacgctg ggctatgccg gctggggcgc
    13561 cggccagctc gaatacgaaa tgagccagaa cgcctggctc agcgtcggcg ccgacccgcg
    13621 catcatcttc gacgtgccgc ccgaggcgcg ctaccccgag gcgctcaagc tgctgggcgt
    13681 cgatccggtc atgctgtccg gcggcgcggg ccatgcctga tacgagcccc caaggcggac
    13741 gcgaagccac cctgctgggc tttgacttcg gcgagaagaa aatcggcgtg gccatcggca
    13801 acacgctcac gcgccaggcc cggccgctcg aaatcatctt ttccgagctg cgcgaggccc
    13861 gcttcgggcg catcgcgcag ctgctggccg aatggcagcc gcaatgcctg gtggtcgggc
    13921 tggcgctggc cgccgacggc ggcgagcagc ccgccacggc gcgctgccgg cgcttcgcca
    13981 accagctcaa cggccgcttc ggcctgcccg tcgtcctggt cgacgagcgc ggctccagcc
    14041 tggaagcgca ggacttgttg aaaggcatgg cccccgcgcc gcgcccttcg ggcttgctgc
    14101 cccccaaggg gggcgtgcct gccttggggc ggcccggcgg caggcacgcc gccgacgacg
    14161 ccatggccgc catgatcatc ctgcagcgcc atctggatac attgccgtaa tgtgacggaa
    14221 cggtgacacg gggccgggta tgatgctgcc cggttacctg tttgcggtca cggagacttc
    14281 cacttgaatc tgttgcgttt catcgcgcgg ctatgcttgg tcgtgccctg gatattcatg
    14341 ggcctggtga tcatcgccct ggtctacccg tggatatcgc ccgtcccgac ccgcgccgcc
    14401 atcaacctct ggtggtcgcg ccgcctcatg cgcatgtgcg gcgtgaaaat cacggtcatc
    14461 ggccaggcgc gcatgtcggg gccggtgctg tgggtggcca accacgtgtc gtggatcgac
    14521 atcttcgtgg tcaattccgt gcgcgccacc gccttcgtcg ccaagtccga aatccgcgcc
    14581 tggcccttca tcggctggct cgccaccggc gccggcacgc tcttcatcga ccgcaaccat
    14641 cgccatgccg tgcacgacat gggcagcgcg attcaaaaat gcttcgacaa cggcgaggcg
    14701 gtggggctct ttcccgaggg caccacgacc gaaggcttcg ggctgcgtcc tttccacgcc
    14761 agcctgttcg agccggcccg ccagtccggg gtggacatcc agcccgtggc catgcgtttc
    14821 ttccaccacg gccagcgcag cagcttcgcc gctttcgtcg gcgaggaaac gctggtggcc
    14881 aatctgtggc gcgtgctggg caccacgggg ctgtcggtcg aggtgatctt cctgcccatc
    14941 ctgcacgtgg tcgcggaagg ggatcgcgca gcgcctacgc gcctgcagct ctcgcatgcc
    15001 gcgcgggatg ccatcgaagc gcagttgcct gtcggggagt gcgtggtttg cgttcttgcg
    15061 gcgcgtcggc gctggggcgt cggggtccgg ctgcatcgcg gcggctcggg cgcaagacga
    15121 cgcgccctcg gccccctgcg ggggctgccc gccgctcaac gcagccggac cccgacgccc
    15181 cagcgccgag gctcacaatg tctcagcccg ccgcatgccg ctcgaggcaa ggcggtttat
    15241 tcgagatgtt gcaagtctgt tggaaggcgg ggtgggggcg ccggaccgtc
//
LOCUS       DASWPO010000108         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3058964, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000108 DASWPO010000000
VERSION     DASWPO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3058964"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /gene="hppD"
                     /locus_tag="VGC69_06330"
     CDS             <1..623
                     /gene="hppD"
                     /locus_tag="VGC69_06330"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY0294936.1"
                     /translation="LNYPGAGLVMIDHLTHNVYKGRMAEWADFYSRLFNFREIRYFDI
                     EGKVTGVKSKAMTSPCGNIRIPINEEGTEEKGQIQEYLDLYHGEGIQHIALATDNIYE
                     TVEKLRKNAVVFLDTPETYYELLEKRLPGHGEDTARLNKNRILLDGAPGGGLLLQIFT
                     ENQIGPIFFEIIQRKGNDGFGEGNFKALFESIELDQMRRGVVRNVA"
     gene            complement(734..1297)
                     /gene="msrA"
                     /locus_tag="VGC69_06335"
     CDS             complement(734..1297)
                     /gene="msrA"
                     /locus_tag="VGC69_06335"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0294937.1"
                     /translation="MSELPITAGHEVAVLGGGCFWCVEAVFENLRGVASVLPGYSGGH
                     VDHPTYEQVCGKDTGHIEVARVEFDPKELSYADLLRVFFATHDPTTPGRQGNDVGPQY
                     ESAIFWQSEAQREQAQAVIAEIDGLHLYDAPIVTKLIPAATLWLAEDYHRHYFELHPD
                     QGYCAFVIAPKVAKFRKQFHDRLKQGA"
     gene            complement(1319..1630)
                     /locus_tag="VGC69_06340"
     CDS             complement(1319..1630)
                     /locus_tag="VGC69_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0294938.1"
                     /translation="MLGLLHVGLLPIAARAQSGADLAASKNCLACHQVDTKRVGPPFR
                     SVAERYAGQSTAMDYLTDKVRHGSRGDWGAVPMPAQTQVSEADAKALVQWVLSLSSSQ
                     P"
     gene            1798..2586
                     /gene="lgt"
                     /locus_tag="VGC69_06345"
     CDS             1798..2586
                     /gene="lgt"
                     /locus_tag="VGC69_06345"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0294939.1"
                     /translation="MLQYPHIDPIAVQIGPLAVHWYGLMYLVGFGLAYLLGRWRITHG
                     HTTFMTVRDLEDLIFYSVLGVVLGGRLGYVLFYKPGYYLTHPLDIFHVWEGGMSFHGG
                     FLGVVLVMLWLAHKKKVSLFTLSDFVAPLIPLGLAAGRLGNFINGELWGRPTSVPWAM
                     VFPQAGDGLPRHPSQLYELGLEGLALFALLWLFSSRPRPTGQVSGLFLIGYGLFRFLV
                     EFTREPDDFLGLLAGGLSMGQWLSLPMVLIGLAVFAITARRSSR"
     gene            complement(2561..3280)
                     /locus_tag="VGC69_06350"
     CDS             complement(2561..3280)
                     /locus_tag="VGC69_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936857.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0294940.1"
                     /translation="MRFPSSHAMAHYAAQAVLCLLAFNVHAATPDASPAGTRLTLQAT
                     ASSEIPLDTVRITLAAETQSADQTTASQQLAAALDAAAKQAQGAAGIVSHTGNYGVWP
                     NHDNQGKINNWHGRGEIVLESKDFAAASALATRMSDKVAISNIAFILSHEARAAEEHQ
                     LLGQVAQAFQARALDAAKAFGFSSYRMVELDLGGAGAAPPMPHPAAMMRMAKTADAID
                     APLEGGSVVVSVSVNGTIALQ"
     gene            complement(3345..3528)
                     /gene="ssrS"
                     /locus_tag="VGC69_06355"
     ncRNA           complement(3345..3528)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGC69_06355"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3596..3907)
                     /locus_tag="VGC69_06360"
     CDS             complement(3596..3907)
                     /locus_tag="VGC69_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY0294941.1"
                     /translation="MERVDVTILGREYSLACELEEKPKLLAAVRHVDQLMVRIQGVGK
                     ISSNERIAVMAALQIAGELLGMKAPDGPLGGLAVGDFKRKIDDMNSMLDDALSGQEKL
                     L"
     gene            complement(3907..4353)
                     /locus_tag="VGC69_06365"
     CDS             complement(3907..4353)
                     /locus_tag="VGC69_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294942.1"
                     /translation="MLQDLDQLAARIGLLVQRTRQVHTERDALRARLNDSELQQRNLQ
                     QRCADHEAELQSLRAAVAEHGQAAETVRAEAGQVEAALREQLAHESAERQALEERLKH
                     REAELQASLQARDSDLNRLRTAALSARERIDVVLSRLPGASVGDQA"
     gene            4548..5012
                     /locus_tag="VGC69_06370"
     CDS             4548..5012
                     /locus_tag="VGC69_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY0294943.1"
                     /translation="MQLRNKQDFWSGAMFIALGLGFAWKASQYQMGTSARMGPGYFPF
                     WLGMALALLGLVVLLSAMAKKADKTEVAPFDWRILLLVIGSVVLYALVLHLLGVYASV
                     FLLVMVSSLASHEFNWKVAVANALFLVAFAYLAFIKGLGLIFPLWPSFLGMN"
BASE COUNT          901 a         1588 c         1676 g         1002 t
ORIGIN      
        1 atctgaacta ccccggcgca ggcctggtga tgatcgacca cctgacgcac aatgtgtaca
       61 agggccgcat ggcggagtgg gccgatttct actcgcgcct gttcaacttc cgcgagatcc
      121 gctacttcga tatcgaaggc aaggtaacgg gcgtgaagtc caaggccatg acctcgccct
      181 gcggcaatat ccgcatcccg atcaacgaag aaggcaccga agagaaaggc cagatccagg
      241 aatacctgga cctctaccat ggcgaaggca tccagcacat tgcgctggcc accgacaata
      301 tctacgaaac ggtggaaaag ctgcgcaaga atgcggtggt cttcctggac acgccggaaa
      361 cgtattacga actgctggaa aagcgcctgc ccggccatgg cgaagacacg gcgcggttga
      421 acaagaaccg catcctgctc gacggcgccc ccggtggcgg cctgctgctg cagatcttca
      481 ccgaaaacca gatcggcccg atcttcttcg agatcattca gcgcaagggc aacgatggct
      541 tcggcgaagg caacttcaag gccttgttcg aatcgatcga gctcgaccag atgcgccgcg
      601 gcgtggtgcg caacgtcgcc tgagcgaccc gcttgagcaa ccctggggcg ggcgcatgac
      661 ccgccgatgc aatcgctgaa acccaaaagg ccagtccaca tggactggcc ttttgctttg
      721 cggcaccagg aacctaggcg ccctgcttca gccgatcgtg gaactgcttg cggaacttgg
      781 cgaccttggg cgcgatgacg aatgcgcaat agccctggtc gggatgcagt tcgaaatagt
      841 gccgatggta gtcctccgca agccagaggg tggccgcggg gatcagcttg gtgacgatgg
      901 gcgcatcgta gagatgcagc ccgtcgatct cggcgatgac ggcctgcgcc tgttcgcgct
      961 gcgcttcgct ctgccagaag atggccgact cgtactgcgg accgacgtcg ttgccctggc
     1021 ggcccggcgt agtggggtcg tgcgtggcga agaagacgcg cagcaggtcg gcgtacgaca
     1081 attccttggg atcgaactcg acgcgggcca cttcgatatg gcccgtgtcc ttgccgcaga
     1141 cctgttcata cgtgggatgg tcgacatggc cgccgctgta gccgggcagc acgctggcga
     1201 cgccgcgcag gttctcgaac acggcttcga cgcaccagaa gcagccgccg ccgagcacgg
     1261 cgacttcatg gccagcggta atgggcagtt ccgacatgac tgttctcgct caaaaggttc
     1321 agggttggga cgacgacagc gacagcaccc actggaccag cgccttggcg tcggcctcgc
     1381 tgacctgcgt ctgggcgggc atgggtaccg cgccccagtc gccgcggctg ccgtggcgga
     1441 ctttatcggt caggtagtcc atggccgtgg actggccggc atagcgctcg gccacactgc
     1501 gaaaaggcgg accgacacgc ttggtgtcga cttgatggca ggccaggcaa ttcttgctgg
     1561 ccgccagatc ggccccggat tgggcccggg ctgcgatcgg caacaacccc acgtgcaaca
     1621 ggcccagcat caggcaggcc atccggcgag tgatcgcgta catcctgaat tccatcttac
     1681 tcgaaccatc ggcccatgcc gatggcgggc ttcgatggta accgaatcgc gcggccggca
     1741 ccgcaggagc agccgcgctg cggttattat ctggcaactt tttccgacgc agccgccatg
     1801 ctccagtacc ctcatatcga ccccatcgcc gtgcagatcg gaccgctggc cgtacattgg
     1861 tacggcctga tgtacctggt gggtttcggg ctggcgtatc tgctgggccg ctggcgcatc
     1921 acgcacggcc acaccacctt catgacggtg cgcgacctgg aagacctgat cttctattcg
     1981 gtgctgggcg tggtgctggg cggccgcctg ggctacgtgc tgttctacaa gccgggctat
     2041 tacctgacgc acccgctgga catcttccac gtgtgggaag gcggcatgtc gtttcatggc
     2101 gggtttctgg gcgtggtcct ggtcatgctg tggctggcgc acaagaagaa ggtgtcgctt
     2161 ttcacgctga gcgactttgt cgctcccctg atcccgctgg ggctggccgc cggacggctg
     2221 ggcaatttca tcaatggcga gttgtggggc cgaccgacca gcgtgccatg ggccatggtc
     2281 ttcccgcaag ccggcgacgg gctgccgcgc catccctcgc agctctatga gttggggctc
     2341 gaaggcctgg cgctctttgc gctgctgtgg ctgttttctt cccggccgcg ccccacgggc
     2401 caggtgagcg gcttgttcct gatcggctac gggctcttcc gctttctggt ggagttcacg
     2461 cgcgagcccg acgatttcct gggactgctg gcgggcggcc tgagcatggg acagtggctg
     2521 tcgctgccca tggtcctgat cggcctcgcc gtctttgcga tcactgcaag gcgatcgtcc
     2581 cgttgaccga cacgctgacg accacggagc cgccttccaa gggcgcatcg atggcatcgg
     2641 ccgtcttggc catgcgcatc atggccgcgg gatgcggcat gggcggcgcg gcgccggccc
     2701 cgcccagatc cagctccacc atgcgatagc tggaaaagcc gaaggccttg gccgcatcga
     2761 gcgcccgggc ctggaaggcc tgggcgacct ggcccagcag ctggtgctct tccgccgcgc
     2821 gggcctcgtg cgacaggatg aaggcgatgt tggaaatcgc gaccttatcg ctcatccggg
     2881 tggccaatgc cgaggccgcc gcgaaatcct tggattcgag cacgatctcg ccacggccgt
     2941 gccagttgtt gatcttgccc tggttgtcat gattgggcca gacgccatag ttgccggtat
     3001 gcgagacgat gcccgcggcg ccttgcgcct gcttggccgc ggcatcgagc gcggcggcca
     3061 gttgctggct ggcagtcgtc tggtcggcgg actgggtttc agcggccagg gtgatgcgca
     3121 ccgtgtccaa ggggatttcg gacgaagccg tggcctgcag ggtcaggcgc gtgccggcag
     3181 gcgaggcatc tggcgtggcg gcgtggacat tgaaagccag caggcacagc acggcctgtg
     3241 ctgcgtaatg cgccatggcg tgcgaggaag gaaaccgcat ggggagcgct ccaggaaacg
     3301 cgtcaggggg aagcggcagg ccctcgatat gggcctgccg gaataaatgc cccgcgtgtg
     3361 ccgtctaggc cggcatctcg aaccctaagg ttcaagtcgg ccgcgtcggt agggcatcgg
     3421 gctcgtttgc gagaaacgag cacgccagcc ctggcactct gcagcctgta tgccattaag
     3481 cataggttca gagaatgtat ggccaagtca cgagcacggc agggacaaac tggaaaggac
     3541 gacgcgtgcg ggccgaggaa agacagccag ctgaggaaaa gtgcttgcgt gccagctaga
     3601 gcagcttttc ctgcccggag agggcgtcgt ccaacattga attcatgtcg tcgattttac
     3661 gcttgaagtc gcccactgcc aaaccgccca gcggcccgtc gggcgctttc atgccgagca
     3721 gctcgccggc gatctgcaaa gcagccatga cggcgatgcg ttcgttgctg gagattttgc
     3781 ccacgccctg gatgcgcacc atgagttgat cgacatggcg cactgcagcc agcagcttgg
     3841 gtttttcttc gagttcgcag gccagggaat attcgcggcc caggatggtg acgtcgacgc
     3901 gttccatcag gcttgatctc caacggaagc gccgggcagg cgggacagca cgacatcgat
     3961 gcgctcgcgc gcggacaggg cggcggtgcg cagccggttc aggtcggagt cgcgggcctg
     4021 caggctggcc tgaagttcgg cctcgcggtg cttgagccgt tcttcgaggg cctggcgctc
     4081 ggccgactca tgggccagtt gctcgcgcag cgccgcttcg acctgcccag cctcggcgcg
     4141 cacggtctcg gcggcctggc cgtgctcggc cacggctgcg cgcagggact gcaattcggc
     4201 ttcgtggtcg gcgcaacgct gttgcagatt gcgttgctgg agttcgctgt cgtttaagcg
     4261 cgcgcgcaag gcatcgcgtt cggtgtgcac ctggcgggtg cgttgcacca agagcccaat
     4321 acgggcggca agttgatcga gatcctgcag catggtcgct atcgtaaacc atccggcccc
     4381 aatcggggag gttccccgct gatctgattg ttatcccaaa ccgcccgaaa aactcggcct
     4441 gatgtctgat tcctgacaaa aagccttctt tttcctgaat tttagctttt cacaagcttt
     4501 catttacctg tattcttgcc gcacaaaaca cgttggcaga ggagaacatg cagctacgta
     4561 acaaacaaga cttctggtca ggggcaatgt tcatcgccct gggcctggga tttgcctgga
     4621 aggccagcca ataccagatg ggcacctcgg cccggatggg gcccggctat ttccccttct
     4681 ggctcggaat ggccctggcc ctgctgggcc tggtggtcct gctgagcgcc atggccaaga
     4741 aagccgacaa gaccgaggtc gcccctttcg attggcgcat tctgctcctg gtgatcggct
     4801 cggtggtgct gtatgcgctg gtcttgcacc tgctgggcgt ctatgcgtcg gtcttcctgc
     4861 tggtgatggt cagcagcctg gccagccatg agttcaactg gaaggtcgcc gtggccaatg
     4921 cgctcttcct ggtggccttt gcctacctgg ccttcatcaa ggggttgggc ctgatcttcc
     4981 ccttgtggcc ctcgtttctg ggcatgaact aaggagaacc gccatggaat tgctgcaaaa
     5041 cctgctgctg ggcttctccg tcgccgtcac gccggaaaac ctgctgtatg ccctgatggg
     5101 ctgcgtactg ggcacgctga tcggcgtgct gccgggcatc ggcccggtgc ccaccattgc
     5161 catgctg
//
LOCUS       DASWPO010000109        13438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_23232, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000109 DASWPO010000000
VERSION     DASWPO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13438
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23232"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1490)
                     /locus_tag="VGC69_06375"
     CDS             complement(168..1490)
                     /locus_tag="VGC69_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEY0294944.1"
                     /translation="MSNRSAYFPAWRRVDAVAQGSVVAPDERLSWPRTIVMGAQHVVA
                     MFGSTVLAPLLMGFNPNLAILMSGVGTLIFFLFVGGRVPSYLGSSFAFIGGVIAATGY
                     AGSGANAHIGVALGAIMACGLVYALVGLVVWAAGSKSGSGAGWIETLMPPVVTGAIVA
                     VIGLNLAPIAVKGAMGQGGFDTAMALATIVCVGGVAVFARGMAQRLLILLGLILACLL
                     YALCANGLGLGKPMAFSGVAAAAWFGLPAFVAPVFKADAMGLIVPAAVILVAENLGHV
                     KAVGAMTGQHLDRYLGRAFVGDGVATMVSAALGGTGVTTYAENIGVMAVTRIYSTLVF
                     VVAALIAVLLGFSPKFGALIQTIPGPVLGGVSIVVFGLIAVAGARIWVVNGVDFSDNR
                     NLIVAAVTLVLGAGDFTVKLGSFVLGGIGTATFGAILLHALLNRRRPA"
     gene            1624..2094
                     /locus_tag="VGC69_06380"
     CDS             1624..2094
                     /locus_tag="VGC69_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294945.1"
                     /translation="MSSISPISPVSSALGGGANSLTRSLQGTRVKSTAATQDAASASG
                     ATATPTQQGIQLTGSNASASGASGGKSGSASQGGGDASSAASSTDDTTTELQQLEAQL
                     QAIMAQMQSLQGSANPDDPSSTAQVAALSAEEMSIEGQIQTLQAQSTTGAASAA"
     gene            2122..2763
                     /locus_tag="VGC69_06385"
     CDS             2122..2763
                     /locus_tag="VGC69_06385"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0294946.1"
                     /translation="MSEIWFIRHGETDWNLERRLQGWQDIPLNDTGAFQAARLAERLR
                     DDAARTPFAAIYSSDLQRAHATAQTVSTALGLPVRVEPGIRERHFGVLEGLHYDTLDQ
                     VAPEAAAAWKSRDPDRPLQGGETLGEFQARVLGASLDLARRHAGERILVVTHGGALDI
                     IWRQASGIALNEPRHARLLNAAINRVRVDGQGWHMLDWGDDRHIAEDARDDIV"
     gene            complement(2797..3255)
                     /locus_tag="VGC69_06390"
     CDS             complement(2797..3255)
                     /locus_tag="VGC69_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0294947.1"
                     /translation="MKRVFITGAGSGLGLALAKRYAADGATLGLVGRSERTLRELAAS
                     LPGQHGCYALDVRDREALHAAARDFIARNAGHADVVIASAGISAGTVTGELDDYAVFR
                     AIVETNLQATWPPSSLWQMGIAAKLMRLLPDGLYDRLARNAPRKPRRNGP"
     gene            complement(3263..4162)
                     /locus_tag="VGC69_06395"
     CDS             complement(3263..4162)
                     /locus_tag="VGC69_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY0294948.1"
                     /translation="MKWFVLALFIVTATYVHLRGRVRHKFSRQALDHSTFTAPINTFM
                     YAFSAVPKTPYIGLDEFPQLQVLIDHWQEIRAEAVKLFGDGLIKGSSQYNDAGFNSFF
                     KTGWGRFYLKWYDKAPQPSALEFCPYTTRLLADIPGVNAAMFTSLPPGARLPRHRDPY
                     GGSLRFHMGLITPNDPACYIDVDGERYYWRDGKAVMFDETFIHYAENTTDQNRIILFA
                     DIERPMKYRWTQTVNHFLGGLLLRAAASPNQEGDRTGSINRIFGAAYAVRRLGKRIKQ
                     YNRTLYYVLKWAIVLAVLAWIFL"
     gene            complement(4183..7416)
                     /gene="carB"
                     /locus_tag="VGC69_06400"
     CDS             complement(4183..7416)
                     /gene="carB"
                     /locus_tag="VGC69_06400"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0294949.1"
                     /translation="MPKRTDLKSILIIGAGPIIIGQACEFDYSGAQACKALKAEGYRT
                     ILVNSNPATIMTDPETADVTYIEPITWQAVEKIIEREKPDALLPTMGGQTALNCALDL
                     AHHGVLAKHGVELIGANEQAIEKAEDRQKFKQAMTDIGLESAKSGVAHSMDEAWEVHK
                     RIAVETGNSGFPSVIRPSFTMGGSGGGIAYNAEEFEAICRRGLEASPTSELLIEESLL
                     GWKEFEMEVVRDKADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRDASI
                     AVLREIGVDTGGSNVQFAVHPQNGRMIVIEMNPRVSRSSALASKATGFPIAKVAARLA
                     VGYTLDELKNEITGGATPASFEPTIDYVVTKVPRFAFEKFPQADARLTTQMKSVGEVM
                     AIGRTFQESFQKALRGLEVGVDGLNQKTTDREKLQVELGEPGPERIWYVGDAFAQGFT
                     LDEVHALTKIDPWFLSQIKEIVDIELALEQKTLADLDYATLWELKRRGFSDRRLAYLM
                     DSSESEVRKLRHQFNVRPVYKRVDTCAAEFATRTAYMYSTYEEECEAAPTDRKKIIVL
                     GGGPNRIGQGIEFDYCCVHAALALREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTL
                     EDVLEIVHKENPVGMIVQYGGQTPLKLARALEANGVPIIGTSPESIDVAEDRERFQKL
                     LHKLGLRQPPNRTARTENEALAHAVEIGYPLVVRPSYVLGGRAMEIVHEQQDLERYMR
                     EAVKVSNDSPVLLDRFLNNAIEVDVDCLADGETVFIGGVMEHIEQAGVHSGDSACSLP
                     PYSLSQEIIAEIKRQTAVMAKALNVSGLMNVQFAIQGGDVYVLEVNPRASRTVPFVSK
                     ATGLQLAKIAARAMAGRTLAQQGVTKEVVPKYFSVKEAVFPFVKFPGVDTILGPEMKS
                     TGEVMGVGRSFGEAFVKSQLAASVRLPESGVAFISVKNQDKPKAVEVARGLHALGFKL
                     VATRGTAAEIEAAGIPVLLVNKVTEGRPHIVDMIKSGEIALVINTVEERRNAIADSRA
                     IRTQSLANRVTLFTTIAGARAAVEGMQYLRQGLGLQVYPLQELHSQN"
     gene            complement(7433..8623)
                     /gene="carA"
                     /locus_tag="VGC69_06405"
     CDS             complement(7433..8623)
                     /gene="carA"
                     /locus_tag="VGC69_06405"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY0294950.1"
                     /translation="MLPLLFPEGPNGKFERLPPAILALADGTVFRGVSIGAPGYTVAE
                     VVFNTAMTGYQEILTDPSYSGQIVTLTYPHIGNTGVNTEDVESRRVFAAGLVVRDCPA
                     RVSNFRATQSLPEYLAEQGVVAISGIDTRKLTRILRDKGAQGACILVGEDAERAVALA
                     QGFPGMAGQDLAKVVSITGRESWSGGTWQLGQGYTEPAQDKFHVVAYDFGVKRNILRL
                     MADRGCRITLVPAQTPAEEVLKLDPDGIFLANGPGDPEPCDYAIAATRVFLERKLPTF
                     GICLGQQIMGLAVGAKTIKMKTGHHGANHPVQDLQSRRVFITSQNHGFAVDAASLPAN
                     ARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPHDILVLFDRFVGLMQQNPKQSP
                     AASK"
     gene            8864..9814
                     /gene="tal"
                     /locus_tag="VGC69_06410"
     CDS             8864..9814
                     /gene="tal"
                     /locus_tag="VGC69_06410"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY0294951.1"
                     /translation="MTSLLDSLRQYTTVVADTGDFEAMRALRPTDATTNPSLIFKAVQ
                     RDAYRPLLQHTVQAHRGATTAELTDRLLVAFGQEILRIVPGRVSTEVDARLSFDTRAT
                     IERARGLVALYEAAGVPRARVLIKIASTWEGIQAARVLQSEGVHCNLTLLFALPQAAA
                     CADAGVQLISPFVGRIYDWHKKTAGAKWVEAEHAGAQDPGVQSVTGIYRYYKQFGIKT
                     EVMGASFRNTGQILALAGCDLLTISPELLTELAGKEGTVTPALRAGDAGQDMARLPVD
                     EVSFRTLMNEDAMASEKLAEGIRAFVADGRKLDALIDSSS"
     gene            complement(10143..10937)
                     /locus_tag="VGC69_06415"
     CDS             complement(10143..10937)
                     /locus_tag="VGC69_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0294952.1"
                     /translation="MSKSFKQGRAFLRLWQILLLVVILAVWWLASKNPKVAFFFGDPL
                     QVCQHIWAWFVTDGEIYTHLWVTLLETVLSFFIGTISGLGCGLWLGLSPRTAAILDPY
                     IKAANSMPRVILATIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVREVPAVLLDNA
                     RMLGANRKQLLRSVYLPSATSWVFSSLHTSVGLAFVGTVVGEYLGSASGVGYLILQAE
                     GTLDVNTVFAGIVVLTVFALLLDYAVSLAEKPLMRWQPKTGEIEKV"
     gene            complement(10942..11751)
                     /locus_tag="VGC69_06420"
     CDS             complement(10942..11751)
                     /locus_tag="VGC69_06420"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0294953.1"
                     /translation="MAEAALSLEGITCTFVSREGEGRRYTAVQDVNLAIASGEFVSVV
                     GPTGCGKSTLLNVGAGLLAPSSGQIRVFGQPLSGINARAGYMFQGEALLPWRSALDNV
                     IAGLEFSGTPKSQAREAGREWLRRVGLGGSEDRYPHQMSGGMRKRAMLAQTLIRDPDI
                     ILMDEPFSALDIQTRQLMENEVLDLWMAKRKAVLFITHDLDEAIAMSDRVVVLSAGPG
                     THPIGEFTIDLPRPRDVSEVRMHPRFVELHSAIWAVLRDEVLKGYAQQKRA"
     gene            complement(11744..12778)
                     /locus_tag="VGC69_06425"
     CDS             complement(11744..12778)
                     /locus_tag="VGC69_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294954.1"
                     /translation="MSVTRRELFKIAGATGAMIAAPGIVRAQKLEQTKVQVAVGGKAL
                     VYYLPLTIAEQLGYFKDEGLDVRIADFAGGSKALEAVVGGSADVCSGAFEHTIILQAK
                     GQFYRTFVLQGRAPMIVLGAGKKMGYKSPADLKGKKVGVTAPGSSTSMLVNFFLSKNG
                     MKASDVSFIGVGAGAGAVSSLRNGQVDAMSNVDPVVSTLETGGDMDVIVDTRTLKDTL
                     AVFGGNMPAGCLYASQEYLTANPNTAQALANAIVRANKWIQKAGADEIAKRVPPSYLL
                     GQPEVYKASLTKSLGGLSPDGIVPDDGPKTSLAAQASYLPNFDASKIDLSKIWTNDFA
                     KRANEKYPNG"
     gene            complement(12933..13436)
                     /locus_tag="VGC69_06430"
     CDS             complement(12933..13436)
                     /locus_tag="VGC69_06430"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY0294955.1"
                     /translation="MVIVMGVSGAGKSLIGERLAQCLKCDFVDGDAFHSAANKAKMAS
                     GTPLTDEDRRPWLRTIRAAIEEKRQAGNTAVFTCSSLKRSYRDILRNGDQDVCFIYLK
                     GEFGLLHERLGARSGHFFNPALLQSQLDTLEEPGPDEAIEASIAPPADQVVDSVLARL
                     AERFPVA"
BASE COUNT         2312 a         4368 c         4535 g         2223 t
ORIGIN      
        1 gcaaaaataa aaccccggct cgggcaagag ccggggcatt cagcttgtat tgtggtcaag
       61 atcgccaaga attgcaagcg caaaggcttg gccggcccac ccggaaaatg atttttctac
      121 cgagaaatca atatattggc cgcaccgtta taggtcaccc gtattgatca ggcaggcctg
      181 cgccggttga gcagggcatg cagcaaaatg gcgccgaaag tcgccgtgcc gatgccgccc
      241 agcacaaagc tgcccagttt gaccgtgaag tcgcccgcgc ccagcaccag cgtcacggcg
      301 gccacgatga ggttgcggtt gtcggaaaaa tcgacgccgt tgaccaccca gatacgcgcc
      361 cccgccacgg cgatcagccc gaaaaccacg atggacaccc cgcccagcac cggaccggga
      421 atggtctgga tcagcgcgcc gaacttgggc gaaaagccca gcagcacggc gatcagcgcc
      481 gccaccacga aaaccagggt cgaatagatg cgggtgaccg ccatgacgcc gatgttctcg
      541 gcataggtgg tgacccccgt gccgcccagc gcggccgaca ccatggtggc cacgccgtcg
      601 cccacgaagg cccggcccag gtagcggtcg agatgctgcc cggtcatggc gccgaccgcc
      661 ttcacgtggc cgaggttctc cgccaccagg atgacggccg ccggcacgat caggcccatg
      721 gcatcggcct tgaacaccgg cgcgacgaaa gcaggcagcc cgaaccaggc ggcggccgcc
      781 acgccggaga aagccatggg cttgcccagc cccagcccgt tggcgcacag ggcatagagg
      841 aggcaggcca ggatcaggcc cagcaggatc agcaggcgtt gcgccatgcc gcgggcaaag
      901 accgccaccc cgcccacgca gacgatggtg gccagcgcca tggccgtatc gaagccgccc
      961 tgccccatgg cgcccttgac cgcgatgggc gcgaggttca ggccgatcac cgccacgatg
     1021 gcgccggtca ccacgggcgg catcagggtc tcgatccagc cggcgccgct gcccgacttc
     1081 gatccggcgg cccagacgac caggccgacc agcgcataca ccaggccgca ggccatgatc
     1141 gcccccagcg ccacgccgat atgggcgttg gcgccgctcc cggcatagcc cgtggcggcg
     1201 atgacgccgc cgatgaaggc gaagctcgat cccaggtagc tgggcacgcg cccgccgacg
     1261 aagaggaaga aaatcagcgt gcccacgccc gacatcaaaa tggcgagatt gggattgaag
     1321 cccatgagca gcggagccag caccgtggag ccgaacatgg cgaccacgtg ctgggccccc
     1381 atgacgatgg tgcgcggcca ggacaggcgt tcgtcaggcg caacgaccga accctgcgcc
     1441 acggcgtcga cgcgtcgcca ggcggggaaa taggcggagc gattggacat ataatgaaat
     1501 taatagaaat atctgcagac gaggcggcgc gccaagtgta gacgccgccc aagaccaggg
     1561 gcaatattgc ctccaggccg ccctcgggtc gggggcgcca accgcccgtt gccggagtcc
     1621 gccatgtcgt ccatctcgcc gatttctccc gtgtcttccg ccctgggcgg cggcgcgaat
     1681 tcgctgaccc ggtcgctgca gggcacccgc gtcaagagca cggccgcaac gcaagacgcc
     1741 gcgtccgcct ccggcgcaac ggcaaccccc acacagcaag gcatccagct gaccggcagc
     1801 aatgcctcgg ccagcggcgc gtcgggcggc aagtccggct cggcctcgca gggcggcggc
     1861 gatgcctcca gcgccgcctc ctccacggac gacaccacga ccgaactgca gcagctcgag
     1921 gcgcagctgc aggccatcat ggcccagatg cagtccttgc agggaagcgc caacccggac
     1981 gacccgtcct cgacggcgca ggtcgcggcg ctcagcgccg aagaaatgtc cattgaaggc
     2041 cagatccaga ccctgcaggc gcaatccacc acgggtgcgg cctcggcagc ctgatgacag
     2101 ccggcccggc ccgcgcgtcc gatgtccgaa atctggttca tccgccacgg cgaaaccgac
     2161 tggaaccttg agcgccggct gcaaggctgg caagacatac cgctcaatga caccggcgcg
     2221 ttccaggccg cccgcctggc cgagcgcctg cgcgacgatg cggcacgcac gccctttgcc
     2281 gcgatctaca gcagcgatct gcaacgcgcc cacgccacgg cccagaccgt gagcacggcc
     2341 ctcggcttgc ccgtgcgtgt cgaacccggc atccgcgaac gccatttcgg cgtgctggaa
     2401 ggcctgcatt acgacaccct cgaccaggtc gcccccgaag ccgcggccgc ctggaaaagc
     2461 cgcgacccgg atcggccgct gcaaggcggg gaaacactcg gcgaattcca ggcgcgcgtc
     2521 ctcggcgcca gcctggatct ggcccggcgc catgccggcg aacgcatcct ggtggtgacc
     2581 cacggcgggg cgctcgacat catctggcgc caggccagcg gcattgccct gaacgaaccg
     2641 cgccatgccc gcctgctcaa tgccgccatc aaccgcgtgc gcgtcgacgg ccagggctgg
     2701 cacatgctgg actggggcga cgaccggcac atcgccgagg acgcgcgcga cgatatcgtc
     2761 taaacccggc gtccgtgccc gccaccgggc cggccgtcag ggcccgttgc ggcgcggctt
     2821 gcgcggcgcg ttgcgcgcca gccggtcgta caggccgtcg ggcagcaagc gcatgagctt
     2881 ggccgcgatg cccatctgcc aaaggctcga aggtggccat gtggcctgca gattggtttc
     2941 gacgatggcc cggaacacag cgtagtcgtc gagctcgccc gtgaccgtgc cggcactgat
     3001 gcccgcgctg gcgatcacca catcggcatg gcccgcgttg cgcgcgatga agtcccgcgc
     3061 cgcggcgtgc agggcctcgc ggtcgcgcac gtccaaggca tagcagccat gttgtccggg
     3121 cagcgaggcg gccagttcgc gcagggtcct ttcgctgcgg ccgaccaggc ccagcgtggc
     3181 gccgtcggcc gcgtagcgtt tggccagcgc cagccccaat ccgctgccgg cgccggtgat
     3241 gaagacccgt ttcaccggtc gcctacaaga aaatccaggc caggacggcc aggacgatgg
     3301 cccacttcag cacgtagtag agcgtcctgt tgtattgctt gatgcgcttg ccgaggcggc
     3361 gcacggcgta cgccgcaccg aagatgcggt tgatgctgcc agtgcggtcg ccttcctggt
     3421 tgggcgaggc ggcggcgcgc agcaacaggc cgcccaggaa atggttgacc gtctgcgtcc
     3481 agcggtactt catggggcgc tcgatatcgg cgaacaggat gatgcggttc tgatcggtgg
     3541 tgttttcggc gtagtggatg aaagtctcgt cgaacatcac cgccttgccg tcgcgccagt
     3601 aatagcgctc gccatccacg tcgatatagc aggcaggatc gttgggcgtg atcaggccca
     3661 tgtgaaaacg cagcgagccg ccgtagggat cgcgatggcg cggcaagcgg gcgcccggcg
     3721 gcagggacgt gaacatggcg gcattgacgc cggggatgtc cgccagcagc ctggtcgtat
     3781 aggggcagaa ctccagggcc gagggctgcg gcgccttgtc ataccacttg aggtagaagc
     3841 gcccccagcc cgtcttgaag aaggagttga agccggcgtc gttgtactgc gaggaaccct
     3901 tgatcaggcc gtcgccgaag agcttgacgg cctcggcgcg gatttcctgc cagtggtcga
     3961 tcagcacctg cagttgcgga aactcgtcca ggccgatgta cggcgttttc ggcacggccg
     4021 aaaacgcgta catgaaggta ttgatgggcg cggtgaaggt cgagtggtcc agggcctggc
     4081 gcgagaactt gtggcgcacg cggccgcgca agtgcacata ggtggccgtc acgatgaaca
     4141 aggccaggac aaaccacttg atcattgccg atacctctaa cctcaatttt ggctatgcag
     4201 ctcctgcagc gggtagacct gaaggcccag cccctggcgc agatactgca tgccttcgac
     4261 cgcggcgcgg gcgccggcga tcgtggtgaa taacgtgacc cggttggcca gtgactgggt
     4321 gcggatggca cgcgagtcgg cgatggcgtt gcggcgctct tcgaccgtgt tgatcaccag
     4381 cgcgatttcg ccgctcttga tcatgtcgac gatatgcgga cggccttcgg tgaccttgtt
     4441 gaccagcagc accgggatgc cggccgcctc gatctcggcg gcggtgccgc gcgtggctac
     4501 cagcttgaag cccagcgcat gcaagccgcg ggccacttcg accgccttgg gcttgtcctg
     4561 gttcttgacg ctgatgaagg cgacgccgga ctcgggcagg cgcacgctgg cggccagctg
     4621 cgacttgacg aaggcttcgc cgaagctgcg gcccacgccc atgacttcgc cggtggactt
     4681 catctcggga cccaggatgg tgtccacgcc cgggaacttg acgaagggaa acacggcttc
     4741 cttcaccgag aagtacttgg gcacgacttc cttggtgacg ccctgctggg ccagggtgcg
     4801 gccggccatg gcgcgcgcgg cgatcttggc cagctgcagg ccggtggcct tggaaacaaa
     4861 gggcaccgtg cgcgaggcgc gcggattgac ttcgagcacg tagacgtcgc cgccctggat
     4921 ggcgaactgc acgttcatca ggccgctgac attgagcgcc ttcgccatga cggccgtctg
     4981 gcgcttgatc tcggcgatga tctcttgcga gagcgagtac ggcggcaggc tgcaggccga
     5041 gtcgcccgag tgcacgccgg cctgctcgat gtgctccatc acgccgccga tgaagacggt
     5101 ctcgccgtcg gccaggcagt cgacgtcgac ttcgatggca ttgttgagga agcggtcgag
     5161 cagcaccggg gagtcgttgc tgaccttcac ggcttcgcgc atatagcgtt cgagatcctg
     5221 ctgctcgtgc acgatttcca tggcgcggcc gcccagcacg tagctggggc gcaccaccag
     5281 cgggtagccg atctcgacgg catgggccag ggcttcgttc tcggtgcgcg cggtgcggtt
     5341 gggcggctgg cgcaggccga gcttgtgcag cagcttctgg aagcgttcgc ggtcttcggc
     5401 cacgtcgatg gactcggggc tggtgccgat gatgggcacg ccgttggctt ccagcgcgcg
     5461 ggcgagcttg agcggggtct ggccgccgta ctgcacgatc atgccgaccg ggttttcctt
     5521 gtgcacgatc tcgagcacgt cttcgagcgt gagcggctcg aagtagaggc ggtcggaggt
     5581 gtcgtagtcg gtggagacgg tttccgggtt gcagttgacc atgatggtct cgtagccgtc
     5641 ttcgcgcagg gccagcgcgg cgtgcacgca gcaatagtcg aactcgatgc cctggccgat
     5701 gcggttggga ccgccgccca gcacgatgat cttcttgcgg tcggtcggcg cggcttcgca
     5761 ctcttcctcg taggtcgagt acatataggc cgtgcgcgtg gcgaactcgg cggcgcaggt
     5821 gtccacgcgc ttgtacacgg gacgcacgtt gaactggtgg cgcagcttgc gcacctcgga
     5881 ctcggacgag tccatcaggt aggccaggcg gcggtcggag aagccgcggc gcttgagctc
     5941 ccagagggtg gcgtagtcga gatcggccag cgtcttctgc tcgagcgcca gctcgatgtc
     6001 gacgatttcc ttgatctgcg acaggaacca ggggtcgatc ttggtcagcg cgtgcacttc
     6061 atcgagcgtg aagccctggg caaaggcgtc gcccacgtac cagatgcgtt ccgggccggg
     6121 ctcgcccagc tcgacctgca gcttctcgcg gtcggtggtc ttctggttca ggccgtccac
     6181 gccgacttcc aggccgcgca gggccttctg gaaggactcc tggaaggtgc ggccgatggc
     6241 catgacctcg cccaccgact tcatctgggt ggtgaggcgg gcgtcggcct gcgggaattt
     6301 ctcgaacgcg aaacgcggca ccttggtgac cacgtagtcg atggtgggct cgaacgaggc
     6361 gggcgtggcg ccgccggtga tttcgttctt gagctcgtcg agcgtgtagc ccacggccag
     6421 gcgcgcggcg accttggcga tgggaaagcc cgtggccttg gaggccaggg ccgacgagcg
     6481 cgacacgcgc gggttcatct cgatgacgat catgcggccg ttctgggggt ggaccgcgaa
     6541 ctgcacgttc gagccgcccg tgtccacgcc gatctcgcgc agcaccgcga tggaggcgtc
     6601 gcgcatgatc tggtattcct tgtccgtcag cgtctgcgcc ggcgcgaccg tgatcgagtc
     6661 gccggtatgc acgcccatgg gatcgaggtt ctcgatcgag cagacgatga tgcagttgtc
     6721 cgccttgtcg cggaccactt ccatttcgaa ctccttccag cccagcagcg actcttcgat
     6781 cagcagctcg ctggtgggcg aggcctccag gccgcggcgg cagatggcct cgaactcctc
     6841 ggcgttgtag gcgatgccgc cgcccgaacc gcccatggtg aagctggggc ggatgacgga
     6901 cggaaagccc gagttgccgg tttccacggc gatgcgctta tgcacttccc aggcttcgtc
     6961 catgctgtgg gccacgcccg acttggccga ttccaggccg atgtcggtca tggcctgctt
     7021 gaacttctgg cggtcttcgg ccttttcgat ggcttgctcg ttggcgccga tcagctcgac
     7081 gccgtgcttg gccagcacgc cgtggtgggc caggtcgagc gcgcagttca gcgccgtctg
     7141 gccgcccatg gtgggcagga gcgcatcggg cttctcgcgc tcgatgatct tctcgaccgc
     7201 ctgccaggtg atgggctcga tgtaggtgac gtcggccgtt tccgggtcgg tcatgatggt
     7261 ggcggggtta ctgttgacca ggatggtgcg gtagccctcg gccttgagcg ccttgcacgc
     7321 ttgcgcgccg gaatagtcga actcgcaggc ctgcccgatg atgatggggc cggcgccgat
     7381 gatcaggatg ctttttaggt ctgtacgctt gggcatggtg gaatcttgtt atttatttgc
     7441 tggccgccgg gctttgcttc gggttttgct gcatcaggcc gacgaacctg tcgaaaagca
     7501 cgaggatgtc gtgcgggccg gggctggcct cggggtggcc ctggaagcaa aaggccgggc
     7561 ggtcggtgag ctcgaatccc tgcagcgtgc cgtcgaacag cgacacgtgc gtgacgcgcg
     7621 cgttggcggg caggctggcg gcgtcgaccg cgaagccgtg gttctggctg gtgatgaaca
     7681 cgcggcgcga ttgcaggtcc tgcacggggt ggttggcgcc gtggtggccg gtcttcatct
     7741 tgatggtctt ggcgcccacg gccaggccca tgatctgctg gcccaggcag atgccgaagg
     7801 tgggcagctt gcgctcgagg aacacgcggg tcgccgcgat ggcgtagtcg cagggctccg
     7861 gatcgccggg gccgttggcc aggaagatgc cgtcgggatc gagcttgagc acctcttcgg
     7921 ccggcgtctg cgccggcacc agggtgatgc ggcagccgcg gtcggccatc aggcgcagga
     7981 tgttgcgctt gacgccgaag tcgtaggcca ccacgtggaa tttgtcctgc gcgggctcgg
     8041 tgtagccctg gcccagttgc caggtcccgc ccgaccagct ctcgcgcccg gtgatggaca
     8101 ccaccttggc caggtcctgc ccggccatgc cgggaaagcc ctgggccagc gccaccgcgc
     8161 gctcggcgtc ctcgcccacc aggatgcagg cgccctgggc gcccttgtcg cgcaggatgc
     8221 gggtgagctt gcgggtgtcg atgccggaga tggccaccac gccctgctcg gccaggtatt
     8281 cgggcagcga ttgcgtggcg cggaagttgg acacgcgcgc cgggcagtcg cgcaccacca
     8341 ggccggcggc gaacacgcgg cgcgattcca cgtcttccgt gttgacgccg gtgttgccga
     8401 tgtgcgggta ggtcagcgtg acgatctggc cgctgtagct ggggtcggtg aggatttcct
     8461 ggtagcccgt catggcggtg ttgaacacca cctcggccac cgtgtagccc ggcgcgccga
     8521 tggacacgcc tcggaaaaca gtgccatccg ccagggccaa tatcgcggga gggaggcgtt
     8581 cgaatttacc attcggtccc tcgggaaaaa gtagcggcag cacggtcaga ccccagtgtc
     8641 aaaaatcgat aatcaaacct tcaaattata gctcaaacgc gggttttccc cgaagaaccg
     8701 cgccaaataa ggctgaaacc gggcttttcc cagtcgggaa cataaggcgt catatcgtat
     8761 cgggacgtga accgccgaac gccggcagga ctctgatgat aggcttgcga acgtgccttc
     8821 tcgcacaccc cgccccatga ttcacgccgc atcgatacac gccatgacca gtctgctcga
     8881 ctccctgcgc cagtacacca ccgtggttgc cgacaccggc gacttcgagg ccatgcgcgc
     8941 gctgcgcccc accgacgcca ccaccaatcc gtcactgata ttcaaggcgg tgcagcgcga
     9001 tgcctatcgc ccgctgctcc agcataccgt ccaggcgcat cgcggcgcca ccactgccga
     9061 gctcaccgac cgcctgctgg tggccttcgg ccaggagatc ctgcgcatcg tgccgggccg
     9121 ggtgtcgacc gaggtcgacg cgcgcctgtc cttcgacacc cgcgccacca tcgagcgcgc
     9181 ccgcggcctg gtcgccctgt acgaagcggc cggggtgccg cgcgcacgcg tgctgatcaa
     9241 gatcgcctcc acctgggaag gcatccaggc ggcgcgcgtg ctgcagtccg aaggcgtgca
     9301 ttgcaacctg acgctgctct tcgccctgcc ccaggcggcc gcctgcgccg acgcgggcgt
     9361 gcaactgatc tcgcccttcg tgggccgcat ctacgactgg cacaagaaaa cggccggcgc
     9421 aaaatgggtg gaagccgaac atgccggggc gcaagatccg ggcgtgcagt cggtgacggg
     9481 catctaccgc tattacaagc agttcggcat caagaccgaa gtgatgggcg caagctttcg
     9541 caacaccggg cagatcctgg cgctggcggg ttgcgacctg ctgacgatca gtcccgaatt
     9601 gctgaccgaa ctggccggca aagaaggcac ggtgacgccg gcgctacgcg ctggcgatgc
     9661 gggtcaggat atggcccgcc tacccgtgga cgaggtgagc tttcgcacgc tgatgaacga
     9721 ggatgccatg gccagcgaga agcttgccga gggcattcgg gcgtttgtgg cggatgggcg
     9781 gaagctggat gcattgattg attcgagttc ttgagacgtc cgccgggagg cgcagcgggg
     9841 ttgccggacc atcggcgggg ctcgggcgcg ccatcaggcg ccctcggtcc ccttcgggga
     9901 cttcccccgc ctcaaaggtc cggcaacccc gctgcgcctc ccggcggact catcacattt
     9961 cgaattgaaa cgtctcaagc ttgaggcacc atgagcattg ccgctggggt gccggggtcc
    10021 ggctgcgttg agcggcgggc agccccgtag gggccgaggg cgcgtcgtct tgcgcccgag
    10081 ccgctgcgat gcagccggac cccggcaccc cagcggcaat gcggctcaag aacccaagat
    10141 cctcagacct tctcaatctc acccgtcttg ggctgccaac gcatcaaggg cttctcggcc
    10201 aaactcaccg cataatcgag cagcagcgcg aacaccgtca gcaccacgat gccggcgaac
    10261 accgtattca catccagcgt gccctcggcc tgcaggatca aataccccac cccgctggcc
    10321 gagcccaggt attcgcccac caccgtgccc acgaacgcca ggcccaccga cgtgtgcagg
    10381 ctggagaaca cccagctcgt ggccgaaggc aggtagacgc tgcgcagcag ctgcttgcgg
    10441 ttggcgccca gcatgcgggc gttgtccagc agcaccgccg gcacctcgcg cacgccctgg
    10501 tagacattga agaacacgat gaagaacacc agcgtcacgg ccagcgccac cttggaccag
    10561 atgcccaggc cgaaccacat ggcgaagatc gtcgccagga tgacgcgcgg catggagttg
    10621 gccgccttga tgtaggggtc gaggatcgcc gcggtccgcg gcgacaggcc cagccacagg
    10681 ccgcagccca ggccgctgat ggtgccgatg aaaaacgaca gcacggtctc gagcagcgtc
    10741 acccacaggt gggtgtagat ctcgccgtcg gtgacgaacc aggcccagat gtgctggcat
    10801 acctggagcg gatcgccgaa gaaaaacgcc accttgggat tcttcgaggc caaccaccag
    10861 acggccagga tcacgaccag caacaggatc tgccacaggc gcaggaaggc gcgcccttgc
    10921 ttgaacgact tggacatggc tttatgctcg cttctgctgg gcataaccct tgaggacctc
    10981 gtcgcgcagc acggcccaga tggcggagtg cagttcgacg aagcgcgggt gcatgcgcac
    11041 ttccgacaca tcgcgcggac gcggcagatc gatggtgaat tcgccgatgg ggtgcgtgcc
    11101 cggcccggcc gacagcacca ccacgcggtc gctcatggca atggcctcgt ccaggtcgtg
    11161 cgtgatgaag agcacggcct tgcgcttggc catccagagg tcgagcacct cgttttccat
    11221 cagttggcgg gtctggatgt cgagcgcgga gaacggttcg tccatgagga tgatgtcggg
    11281 gtcgcggatg agggtctgcg ccagcatcgc gcgcttgcgc atgccgcccg acatctggtg
    11341 cggatagcgg tcttccgaac cgcccaggcc gacgcggcgc agccactcgc ggccggcttc
    11401 gcgcgcctgg gacttgggcg tgcccgagaa ttccaggccg gcgatcacat tgtccagcgc
    11461 gctgcgccag ggcagcaagg cctcgccctg gaacatatag ccggcccgcg cattgatgcc
    11521 cgacaaaggc tggccgaaaa cacggatctg ccctgaagac ggggccagca atccggcgcc
    11581 gacgttgagc agcgtggatt tgccgcaacc cgtggggccg acgaccgaaa cgaattcgcc
    11641 ggaggcgatg gccagattga cgtcctgcac ggccgtgtag cgccggccct cgccctcgcg
    11701 cgacacgaac gtgcaggtga ttccttccaa agacagtgca gcttcagcca ttgggatatt
    11761 tttcgttggc gcgcttggcg aaatcgttgg tccagatttt cgacaggtcg atcttgctgg
    11821 cgtcgaagtt gggcaggtag gacgcctggg cggcgagcga ggtcttggga ccgtcgtcgg
    11881 gcacgatgcc gtcgggcgac aggccgccca ggctcttggt gagcgaagcc ttgtagacct
    11941 cgggctgccc cagcaggtag gacgggggca cgcgcttggc gatttcgtcg gcgccggcct
    12001 tctggatcca cttgttggcg cgcacgatgg cgttggcgag cgcctgggcc gtattgggat
    12061 tggccgtcag gtactcctgc gaggcgtaca ggcagccggc gggcatgttg ccgccgaaca
    12121 cggccagcgt gtccttgagc gtgcgggtgt cgacgatgac gtccatgtcg ccgccggtct
    12181 cgagggtgga aaccaccgga tcgacgttgg acatggcatc gacctgccca ttgcgcagcg
    12241 aggagaccgc cccggccccg gcgcccacgc cgatgaagga gacgtcggag gccttcatgc
    12301 cgttcttgga caggaagaaa ttgaccagca tgctggtcga cgagcccggc gccgtcacgc
    12361 cgaccttctt gcccttgagg tcggccgggc tcttgtagcc catcttcttg ccggcgccga
    12421 gcacgatcat gggcgcgcgg ccctgcagca cgaaggtgcg gtagaactgg cccttggcct
    12481 gcaggatgat ggtgtgctcg aaggcgcccg agcagacgtc ggcgctgccg cccaccacgg
    12541 cctccagggc cttggaaccg ccggcgaagt cggcgatgcg cacgtccagc ccctcgtcct
    12601 tgaaatagcc gagctgctcg gcgatggtca gcggcaggta atagaccagg gccttgccgc
    12661 cgacggcgac ctgcaccttg gtttgttcga gtttctgggc gcggacgatg cctggcgccg
    12721 cgatcatggc accggtggcg ccggcgatct tgaacagctc acgacgggtg actgacatga
    12781 tgtgtctcca agtagttata ggtatggacc gatctgagcc ggcattgcgc cagataatac
    12841 cccagagacc gcaagggctt gcttggtggc aaccccaagc aagccccggg caaagacggc
    12901 caaattcgtg ccagctgatt gtcaggtagc cctcaggcga ccgggaagcg ttcggccagc
    12961 cgcgccagga cgctgtcgac gacctggtcc gccggcggcg cgatgctggc ctcgatggcc
    13021 tcgtctgggc cgggctcttc cagcgtgtcg agctggctct gcagcagcgc gggattgaag
    13081 aagtggccgc tgcgcgcgcc caggcgctcg tgcagcaggc cgaactcccc cttcaggtag
    13141 atgaagcaca cgtcttggtc gccgttgcgc aatatgtcgc gataggagcg cttgagcgac
    13201 gagcaggtga agaccgcggt gttccctgcc tgccgttttt cctcgatggc cgcgcggatg
    13261 gtgcgcagcc agggccggcg gtcctcgtcg gtcagcggcg tgccgctggc catcttggcc
    13321 ttgttggccg cgctgtggaa ggcgtcgcca tcgacgaagt cgcacttcag gcactgggcc
    13381 aggcgttcgc cgatgagcga tttgccggcg cccgagacgc ccatgacgat gacgatca
//
LOCUS       DASWPO010000110         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_470366, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000110 DASWPO010000000
VERSION     DASWPO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470366"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1233)
                     /locus_tag="VGC69_06435"
     CDS             complement(139..1233)
                     /locus_tag="VGC69_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0294956.1"
                     /translation="MAEATASRRVAGRAVLWAGLLLGLATAAQAGEGPLLLSTRAAPA
                     LPGSGGSWGFAALDPTRPYLFIARRENGLTVFDVRQRKVVRQVPGTRGANGVAFVPRR
                     NRAYVPNMDGTLTVVRLSDMKALARIPVARGNLNQAVYEPRSDRLLITSGRRADVSTI
                     YQLDPATDRIVAQSDIAAKKLDPPMPLPDGRVLLPWRDEGEVAVWDPRSAQTLASWRY
                     PACARPSALAHDGQGRVFIACRGAKPVLIVADAATGEQKAALPLLRNVNALAYDPARR
                     WVLAPSGDDAALNVYLRQSPDDYRLLGQVGTRPWAHNMVLAPGRGEAYLFTADFTQRV
                     AEPGAERPSPDFHPDTFTVLVLGIDPSSGK"
     gene            complement(1257..>3150)
                     /locus_tag="VGC69_06440"
     CDS             complement(1257..>3150)
                     /locus_tag="VGC69_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0294957.1"
                     /translation="DDALANVSGITQGNTLAGTQDAIMLRGFGDNRDGAIMRDGMPVV
                     QGRALNVTADSVEVLKGPASLLYGIMDPGGVVNVTPKRPQLDRYNALTMTGSSYGTGK
                     NGGGLTLDSTGAIGNSHLAYRLIADYTNEAYWRNFGTHREALIAPSLAWIDDDTQVVL
                     SYEHRNFLYPFDRGTALDPRTNRPLDIPADRRLDAYNNDMWGQSNLLQLDADHRFNSR
                     WKAHLGYSFNRETYDANQMRIVSVNPGTRTLSRSNDGTQGADSTDSFLTGSLEGNFDV
                     AGMRNDVQFGTDFENRKIYRSNLLRQATRCTFSYLNPDYGCEPQTTTVSPPDSDQTDK
                     LQAVSFWLQDSLHLTDKWILVGGLRYQHWDQLAGKGRPFHVNTDTDDGKLLPRVGLVY
                     KMNDALSFYGSYTESLKPTSTIAALSSGTVVDSSIQPEQSKSWEVGAKWDMEDGLSGT
                     VALFDIRKRNVLVSQFNDVTKLTDWRTSGAARSRGIEVDLSGKLSQRWSAIASYAYLD
                     AKTTEDPDFAGKRLWNVARNTASLNLVYDAGPVLGNGRLRLGGGAHYEGGRPGDSANS
                     FMLPSYVVFNAFATYDTKISGHNAHIQFNVNNLFNRVYYPSSANQYFVAVGDARQFIL
                     RTTIEF"
BASE COUNT          487 a         1003 c         1118 g          542 t
ORIGIN      
        1 aggcgcccca caatcccagc caaccatgcg tcttgcgcag ccaattgatg aacacgcccc
       61 ggcgagcgcc ggcgcgcggc ctggtcttgc caggcgcctg gggggaggca gcagaggagg
      121 ccatgcgata ggtcgacgtc atttaccgga agaaggatcg atgccaagca ccagcacggt
      181 gaaggtgtcc ggatgaaaat cgggcgaggg ccgctcggcg cccggttcgg caacgcgttg
      241 cgtgaaatcc gcggtaaaca gataggcctc gccacgcccg ggcgcaagca ccatgttgtg
      301 agcccagggg cgtgtgccca cctggcccag caaacgataa tcgtccggcg attgccgcag
      361 gtagacattg agcgccgcat cgtcgccgct cggcgccagt acccagcggc gggctgggtc
      421 gtaggccagg gcattgacgt tgcgcagcag cggcagcgcg gccttctgtt cgcctgtggc
      481 ggcgtcggcc acgatcagca cgggcttggc gccacggcag gcgatgaaga cgcgcccttg
      541 gccatcgtgg gccagggcgc tgggcctggc gcaggcggga tagcgccagg aggccagcgt
      601 ctgcgcgctg cgcggatccc agacggcgac ttcgccttcg tcgcgccacg gcagcaacac
      661 ccggccgtcc ggcagcggca tcggcgggtc cagctttttg gccgcgatgt ccgactgggc
      721 gacgatgcga tccgtggccg ggtccagctg gtagatcgtc gagacatccg cgcgccgacc
      781 gctggtgatg agcagccggt cgctgcgggg ctcgtagacc gcctggttca gattgccccg
      841 cgccaccggg atgcgcgcca gcgccttcat gtcggacagg cgcacaaccg tcagcgtgcc
      901 gtccatattg ggcacatagg cccggttgcg ccgcggcacg aaggccacgc cattggcgcc
      961 tcgcgtcccc gggacctgcc tgacgacttt gcgctgccgc acgtcgaaca cggtcaagcc
     1021 gttttcgcgc cgggcgatga acaggtaggg ccgggtcgga tcgagcgcgg cgaagcccca
     1081 gctgcctccg gaacccggca acgccggcgc tgcgcgggtg ctcagcagca gcgggccttc
     1141 gccggcctgc gcggcggttg ccaggcccag caacagccct gcccataaaa ccgcccgccc
     1201 ggccacgcgc cgggatgctg tcgcctcggc catccatcgt cgcatcgcgg ccaccctcaa
     1261 aactcgatgg tcgttcgcag gatgaactgc ctggcgtccc ctacggcgac gaagtactga
     1321 ttggcgctgg aggggtagta cacgcggttg aacaggttgt tcacgttgaa ctggatgtgc
     1381 gcgttgtgcc cgctgatctt cgtgtcgtag gtggcgaagg cgttgaacac gacataggac
     1441 ggcagcatga agctgttggc cgaatcgccc gggcgccccc cctcgtagtg cgcgccaccg
     1501 cccagccgta ggcggccgtt gcccagcacc gggccggcgt cgtagaccag attgagcgag
     1561 gccgtgttgc gcgccacgtt ccacaggcgc ttgccggcga aatccgggtc ttcggtggtc
     1621 ttggcgtcaa ggtaggcgta gctggcgatc gcgctccagc gctggctgag cttgccgctc
     1681 aggtcgactt cgatgccgcg cgagcgagcc gcgcccgagg tgcgccagtc ggtcagcttg
     1741 gtgacgtcgt tgaattgcga taccagcacg ttgcgcttgc ggatgtcgaa gagcgcgacg
     1801 gtacccgaca ggccgtcttc catgtcccac ttggcgccca cctcccacga cttggactgc
     1861 tcgggctgaa tgctggaatc gaccaccgtg ccgctggaca atgcggcgat ggtggacgtg
     1921 ggcttgagcg actcggtata gctgccgtag aacgacagcg cgtcgttcat cttgtagacc
     1981 aggccgacgc gcggcagcag cttgccgtcg tcggtgtcgg tattgacgtg gaaaggccgg
     2041 cccttgcccg cgagctggtc ccagtgctgg tagcgcaggc cgcccaccag aatccacttg
     2101 tcggtcaggt gcagcgagtc ctgcagccag aacgacacgg cctgcagctt gtcggtctgg
     2161 tcgctgtcgg ggggcgacac ggtggtggtc tgcggctcgc agccgtagtc cgggttcagg
     2221 tagctgaagg tgcagcgggt ggcctggcgc aacaggttgg aacgatagat cttgcggttc
     2281 tcgaagtccg tgccgaactg cacgtcgttg cgcatgcccg ccacgtcgaa attgccttcc
     2341 agcgaacccg tgaggaaact gtcggtgctg tcggcgcctt gcgtgccgtc attgctgcgc
     2401 gacagcgttc tcgtgcccgg gttaaccgag acgatgcgca tctggttggc gtcgtaggtc
     2461 tcgcggttga agctgtagcc caggtgcgcc ttccagcggc tgttgaagcg gtggtccgca
     2521 tcgagctgga gcaggttcga ctgcccccac atgtcgttgt tgtaggcgtc caggcggcga
     2581 tcagccggga tgtccagcgg acggttggtg cgcggatcca gcgccgtgcc gcggtcgaag
     2641 gggtagagga agttgcggtg ctcgtacgac aggaccacct gcgtgtcgtc gtcgatccag
     2701 gccagcgacg gcgcgatcag ggcctcgcgg tgcgtgccga aattgcgcca gtaggcctcg
     2761 ttggtgtagt cggcgatcag gcgataggcc aggtgggagt tgccaatggc cccggtggag
     2821 tccagcgtca ggccgccgcc gttcttgccc gtgccgtagg acgagcccgt catggtcagc
     2881 gcgttgtagc ggtcgagctg cgggcgcttg ggcgtcacgt tgaccacgcc gcccgggtcc
     2941 atgatgccgt agaggagcga cgccggaccc ttgaggactt ccacgctgtc ggcggtgacg
     3001 ttgagcgcgc ggccctgcac gacgggcatg ccgtcgcgca tgatggcgcc gtcgcgattg
     3061 tcgccgaagc cgcgcagcat gatggcgtcc tgcgtgccgg ccagggtgtt gccctgggtg
     3121 atgccgctga cgttggccag ggcgtcgtcg
//
LOCUS       DASWPO010000111         9645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2434567, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000111 DASWPO010000000
VERSION     DASWPO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434567"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VGC69_06445"
     CDS             complement(<1..701)
                     /locus_tag="VGC69_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HEY0294958.1"
                     /translation="MSATSASSPTTPDAARIIAQLADELKVRAVQIAAAVDLLDDGAT
                     VPFIARYRKEATGGLDDNVLRELEVRLVYLRELETRRLAILESIEQQGKLTPALKQEI
                     ATADTKQRLEDLYAPYKPKRRTRAQIAREAGLEPLADAILADPSCDPAALAAGYLNPE
                     ASITDAKTALDGARDILAERYAENADLLANLREHLWTTGVLYSKVSEGKETEGANFRD
                     WFDFSEPLRTLPSHRV"
     gene            866..2962
                     /locus_tag="VGC69_06450"
     CDS             866..2962
                     /locus_tag="VGC69_06450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0294959.1"
                     /translation="MLDPDIADYFSPAGPLARALPGYRPRQAQIELAEAIDQAMRARG
                     TLVAEAGTGIGKTWAYLVPAIVQGGKVLISTGTRTLQDQLYGRDLPRVREALAMPVSV
                     ALLKGRGNYVCHYHLDRLQGDDRALKSRTEVAQLRKIQFFAGISKTGDRADLGDVPED
                     ADIWQRVTSTRENCLGQDCPRIRDCFVVKARRQAQEADVVVVNHALFMADLVLREEGV
                     TDLLPEADTVIFDEAHQLPDTATRFLGSSVSTHQLMDFGRAMEAAGLAYAREAANWGE
                     VSRQLETAARELRLACAPLERMPGRKATFEAIPEPEVFDVALARLRGVLDAAVRALLG
                     VAEKHPDLMAAGRQGAELCVRLARWATPGRDGKAPPTLESGWGTDANATDPDTDRHNP
                     HIEPAAAFGVEPSTPQGPLVRWVEQGQHHVRLHAAPLSVAQAFSRYRKPGQAWVLTSA
                     TLSVHGDFNHFIAQLGLDDARTARWESPFDYASQALLFVPQGMPEPQSPQFTERFVQT
                     LMPLLEASPGGALVLCTTLRAVERVALGLAGAFEQAGHDWPLLRQGDATRRELLERFA
                     KLEHPVLVGSASFWEGIDLPGEILTLVAIDKLPFAPPDDPVIEARLRACRAQGGNPFA
                     EYQLPEAAISLKQGAGRLIRTEQDWGVLMVGDARLVEKPYGKRLWRGLPPFARTRSLD
                     EALAFYARRAALQEAK"
     gene            complement(3018..3857)
                     /locus_tag="VGC69_06455"
     CDS             complement(3018..3857)
                     /locus_tag="VGC69_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0294960.1"
                     /translation="MYLSAAFSFSGFLRQPALRAAAALSALLLMAGCASNPKADQTAN
                     WSAEQLYSTAKEDISDNRWNDARTHLTAIESRYPFGIYAQQAEIDLAYVNWKDGEVDQ
                     ALGAIDRFMQLYPDHPGADYALYLKGLINFSPAGALATWVSGQDPAERDPKGLRASYD
                     AFAELVKRYPDSRYAPDARLRMTWLVNALAMNEVYVARYYYERGAYLAAANRAQHVIT
                     DFSGVPAAEPALYIMTLSYDKLGMADLKNDTQRVLDTNFPNSTIKKNGLDSLSKKAWW
                     QIF"
     gene            3913..4959
                     /locus_tag="VGC69_06460"
     CDS             3913..4959
                     /locus_tag="VGC69_06460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0294961.1"
                     /translation="MSDTAAGHFSGRTVPAGPAAPDDEPLDDVLGEEAAGGSDLLVVP
                     SGQRADRLDKVLAALLPEHSRSRLQGWIESGHVQVNGAPAKVRSTVGPGDRIEVWAQP
                     APESLAFSPEPVDFAVVADSPDWIVVDKPAGLVTHPGAGNWHGTLLNGLLYRYPELAR
                     VARAGIVHRLDKDTSGLMVVARHDAAQTHLVRQLQARTVGREYLALVHGWLTASGTVD
                     RPIGRDARVPVRMSVERPIAPKPAITHYRTDRLGAVAGDGPHAAASPVTQVICRLETG
                     RTHQIRVHLSSLGHPLLADALYGGRAMAGAARQMLHARALRFDDPGGGEPLSFQSSPP
                     DDMLAVQEAIRWNS"
     gene            4956..5720
                     /gene="pgeF"
                     /locus_tag="VGC69_06465"
     CDS             4956..5720
                     /gene="pgeF"
                     /locus_tag="VGC69_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY0294962.1"
                     /translation="MSVDQVVQGLPVVTGAAWPGVRYFCTTRAGGVGVAPHDTLNLGL
                     RADDRPEAVAENRRRVRAAVPGEPLWLRQVHGAAVMDADAPAAPVEPAADASVTARPG
                     QVLAIMAADCLPVVISDARGSVLGAAHAGWRGLAGGVLENTLRAMQAKQPGAQGWRAW
                     VGPGIGPSAFEVGEDVLQAFAPDGPEALALFAPRPGVPGKWLADLPGLARLRLRRAGV
                     AQPVLSGLCTVSEAGRFFSYRRDRVTGRMALLAWLA"
     gene            5807..7435
                     /gene="phaC"
                     /locus_tag="VGC69_06470"
     CDS             5807..7435
                     /gene="phaC"
                     /locus_tag="VGC69_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY0294963.1"
                     /translation="MSTPLHAVWPGPVSVAPDVLAEIQADFSREWQRLCDEAERGVLA
                     PPRDRRFASVAWASSSQHALIAHAYLLSAQAMSRMVDAAQVSEPLRERLRFALMQWID
                     AMSPANFLAFNPEVQQSIVESAGKTLTMGLGNLLQDVKKGRISQTDETQFEVGRNLAC
                     TPGQVVFENRLMQLIQYAPQTGTVYQRPLVVVPPNINKYYILDLQPDNSFVRHAVEQG
                     HTVFIVSWRNPLATDTDGIDAATWSDYLDDAVLKAISVASEITGQPQVNALGFCVGGT
                     LLASALALAQARGSNPAASLTLLTSLLDFHDTGVLSVFVDEGHALLRDQQLGHGGLMF
                     ARDLATTFSFLRPNELVWNYVVGNYLKGQTPPPFDLLFWNADSTNLPGPFFAWYFRNT
                     YLENNLKVPGKAQASGVPLDLTRLTMPAYLFGSREDHIVPWVSAYASTQLLRGPMRFV
                     LGASGHVAGVVNPPARNKRSYWVAPPGQAGLPGDPNAWLNHAEEHPGSWWTDWAQWLA
                     PHGGKRVRAPGRLGGANYEPIEAAPGRYVKVRAV"
     gene            7520..8260
                     /gene="phbB"
                     /locus_tag="VGC69_06475"
     CDS             7520..8260
                     /gene="phbB"
                     /locus_tag="VGC69_06475"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY0294964.1"
                     /translation="MSSAKLAYVTGGMGGIGTSICQRLAKDGFRVVAGCGPSRNYQQW
                     LDEQAAQGYTFYSSVGNVADWESTVLAFEKVRAEYGVVDVLVNNAGITRDGLFRKMTV
                     DDWRAVIDTNLNSLFNVTKQVIDGMVDKQWGRIISISSVNGQKGQFGQTNYSTAKAGI
                     HGFTMALAQEVAAKGVTANTISPGYIGTDMVRAIRPDVLEKIVATIPVRRLGMPEEIA
                     SIVSWLASDESGFATGADFSLNGGLHMG"
     gene            8324..8887
                     /gene="phaR"
                     /locus_tag="VGC69_06480"
     CDS             8324..8887
                     /gene="phaR"
                     /locus_tag="VGC69_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY0294965.1"
                     /translation="MTQAQKGAAQRLIKKYPNRRLYDTQTSTYITLTDVKQLVLATED
                     FQVIDAKSGDDLTRSILLQIILEEESGGMPMFSSTMLAQIIRFYGHAMQGIMGSYLEK
                     NLQAFMEIQERVAEQSKGLYGTNLGPEAWTQFMNGQAPMLQTMMNNYIDQSKNLFVQM
                     QDQMQDQTRNMFSTFPFVPGSTPGGRK"
     gene            9059..9610
                     /locus_tag="VGC69_06485"
     CDS             9059..9610
                     /locus_tag="VGC69_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEY0294966.1"
                     /translation="MSKRSLALSAGLLGLSLVFAGAAGAAEYPIGKPVQQGGMEVGAV
                     YLQPIEMDPPNVMRAAKDSDIHLEADIHAIADNKTGFPEGEWMPYLVVHYELQKAGSA
                     NVVKGMLMPMVANDGPHYGDNVKLEGPGKYHLKLSVQPPTAEPMNHFGRHIDKETGVG
                     PWFEPLNLDYDFAFAGTGKKGGY"
BASE COUNT         1564 a         3170 c         3322 g         1589 t
ORIGIN      
        1 acgcggtgcg agggcagggt gcgcagcggt tcgctgaaat cgaaccagtc gcggaaattc
       61 gcgccttcgg tttccttgcc ttcggacacc ttggagtaca gcacgccggt ggtccacagg
      121 tgctcgcgca ggttcgccag cagatcggcg ttctcggcgt agcgctcggc caggatgtcg
      181 cgcgcgccgt ccagcgccgt cttggcgtcg gtgatcgagg cctcggggtt gagatagccg
      241 gcggccagcg cggccgggtc gcaggacggg tcggccagga tggcgtcggc cagcggctcc
      301 aggccggctt cgcgggcgat ctgggcgcgg gtgcggcgct tgggcttgta aggggcgtac
      361 aggtcttcca ggcgctgctt ggtgtcggcg gtggcgattt cctgcttgag cgcgggggtg
      421 agcttgccct gctgctcgat cgattccagg atggccaggc ggcgcgtttc gagttcgcgc
      481 aggtagacca ggcgcacttc cagctcgcgc agcacgttgt cgtccaggcc gccggtggcc
      541 tccttgcggt agcgggcgat gaagggcacg gtggcgccgt cgtccagcag gtccacggcg
      601 gcggcgatct ggacggcgcg gactttcagt tcgtcggcca gttgggcaat gatccgggcg
      661 gcgtccggcg tggtggggga ggaggcagag gttgcagaca tctcgctacg gcagaaaaac
      721 agacagaggc ccggattttg ccacaaggcg ggtaaggtca tctggcgata gagggacggt
      781 tcggccacac cggcgcggac ggccggcagg ctggcggcgg tatcatgggg cccctccttg
      841 atccgccggc gctcacgccg ttttcatgct cgacccggac atcgccgatt atttctcgcc
      901 tgccggcccc ctggcccgcg cgctgcctgg ctaccggccc cggcaggcgc agatcgagct
      961 ggccgaggcc atcgaccagg ccatgcgggc gcgcggcacg ctggtggccg aggccggcac
     1021 gggcatcggc aagacctggg cctacctggt gcctgccatc gtgcagggcg gcaaggtgct
     1081 gatctccacc ggtacgcgca ccctgcagga ccagctctac ggccgcgacc tgccgcgggt
     1141 gcgcgaggcg ctggccatgc cggtgtcggt ggccctgctc aaggggcggg gcaattatgt
     1201 ctgccactat catctcgacc ggctgcaggg cgacgaccgg gcgctgaagt cgcgcaccga
     1261 ggtggcgcaa ctgcgcaaga tccaattctt cgccggcatt tccaagacgg gcgaccgggc
     1321 cgatctgggc gatgtgccgg aagacgccga tatctggcag cgtgtgacct ccacgcgcga
     1381 gaactgcctg ggccaggact gcccgcgcat ccgcgactgc ttcgtggtca aggcgcgccg
     1441 ccaggcccag gaagccgacg tggtggtggt caaccatgcc ctgttcatgg ccgacctggt
     1501 gctgcgcgaa gagggcgtca ccgacctgct gcccgaggcc gatacggtga tcttcgacga
     1561 ggcgcaccag ttgcccgaca cggccacgcg tttcctgggc agcagcgtgt ccacgcacca
     1621 gctgatggat ttcggccgcg ccatggaagc ggccggcctg gcctatgcgc gcgaggcggc
     1681 caactggggc gaggtgtcgc gccagctcga gacggccgcg cgcgaactgc gcctggcctg
     1741 cgcgccgctg gaacgcatgc cgggccgcaa ggcgacattc gaggccatcc ccgagcccga
     1801 ggtgttcgac gtggccctgg cgcgattgcg cggcgtgctc gatgccgccg tgcgcgcctt
     1861 gctgggcgtg gcggagaagc accccgactt gatggcggcc ggccgccagg gcgcggagct
     1921 gtgcgtgcgc ctggcgcgct gggccacgcc cgggcgcgac ggcaaggcgc cgccgacgct
     1981 ggaatccggc tggggcacgg acgccaatgc aacggacccg gatacggacc ggcacaaccc
     2041 tcacatcgaa cccgctgccg ccttcggcgt ggagccgtcg accccgcagg gccccctggt
     2101 gcgctgggtc gagcagggcc agcaccatgt gcgcctgcat gccgcgccct tgtcggtggc
     2161 gcaggcgttc tcgcgctacc gcaagccggg gcaggcctgg gtgctgacgt cggccacttt
     2221 gtcggtacac ggcgacttca accatttcat cgcgcagctc ggcctggacg atgcgcggac
     2281 ggcgcgttgg gaatccccct tcgactatgc ctcgcaggcc ctgctgttcg tgccccaggg
     2341 catgcccgag ccgcagtcgc cgcagttcac cgagcgtttc gtgcagaccc tgatgccgct
     2401 gctcgaggcc agcccgggcg gggcgctggt gttgtgcacg acgctgcggg cggtcgagcg
     2461 cgtggccctg ggcctggccg gcgccttcga gcaggcgggc cacgactggc cgctgctgcg
     2521 ccagggcgat gcgacgcggc gcgagctgct cgagcgtttt gccaagctcg agcatcccgt
     2581 gctggtgggc agcgccagct tctgggaagg catcgacctg ccgggagaaa tcctcacgct
     2641 ggtggccatc gacaagctgc ctttcgcgcc gcccgacgac ccggtgatcg aggcgcgcct
     2701 gcgcgcctgc cgcgcccagg gcggcaaccc ctttgccgag taccagttgc ccgaggcggc
     2761 catttcgctc aagcaggggg ccggccgcct gatccgcacc gagcaggatt ggggcgtgct
     2821 gatggtcggc gacgcgcgcc tggtggaaaa accctatggc aagcgcctgt ggcgcggcct
     2881 gccgcctttc gcgcgcaccc gcagcctgga cgaggccttg gcgttctatg cgcggcgggc
     2941 cgcgctgcag gaagccaaat aaatgaagaa gccccctgca agggggcttc ttcgtcatgc
     3001 ggcgataggg cgcgtcgtca gaagatctgc caccaggcct tcttggagag cgagtccagc
     3061 ccgttcttct tgatggtgct gttggggaag ttcgtgtcca gcacgcgctg cgtgtcgttt
     3121 ttcaggtcgg ccatgccgag cttgtcgtag gacagcgtca tgatgtagag cgccggttcg
     3181 gcggccggca cgcccgagaa gtcggtgatg acgtgctggg cccggttggc ggcggccagg
     3241 taggcgccgc gctcgtagta atagcgcgcc acgtagactt cgttcatggc cagcgcattg
     3301 accagccagg tcatgcgcag cctggcgtcc ggggcgtagc ggctgtcggg gtagcgcttg
     3361 accagttcgg caaaggcgtc ataggaagcg cgcaggccct tggggtcgcg ctcggccgga
     3421 tcctggccgg agacccaggt ggccagggcg ccggccggcg agaagttgat caggcccttc
     3481 aggtagagcg cgtagtcggc gcccgggtga tcgggataga gctgcatgaa ccggtcgatg
     3541 gcgcccaagg cctggtcgac ttcgccgtcc ttccagttga cgtaggccag gtcgatctcg
     3601 gcctgctggg catagatgcc gaagggatag cggctttcga tggcggtaag gtgggtgcgg
     3661 gcgtcgttcc agcggttgtc ggaaatgtct tccttggcgg tggaatagag ctgctcggcg
     3721 ctccaattgg cggtctggtc ggccttgggg ttgctggcgc agccagccat cagcagcagg
     3781 gctgacaaag cagcggcggc gcgcagcgcc ggttggcgca ggaagccgga aaacgaaaaa
     3841 gcagccgaga ggtacatcac gggataaatt tccttgatgg taggatgtgc catgattata
     3901 tgatttgtct ccatgtctga tacagccgcc ggccatttct ctggacggac cgttcccgca
     3961 gggccggccg ctcccgatga cgagcccctg gacgacgtcc ttggcgagga ggccgcaggc
     4021 gggtccgatc tgctcgtggt gccgtccgga cagcgcgccg accggctcga caaggtgctg
     4081 gccgcgctcc tgcccgagca ctcccgcagc cgcctgcaag gctggatcga gagcggccac
     4141 gtgcaggtca acggcgcgcc ggccaaggtg cgctcgaccg tggggccggg cgaccggatc
     4201 gaggtctggg cccagcccgc ccccgaaagc ctggcctttt ccccggaacc ggtcgacttt
     4261 gccgtggtgg ccgacagccc cgactggatc gtggtcgaca agccggccgg gctggtgacg
     4321 catcctggcg ccggcaattg gcacggcacg ctgctcaatg gccttttata ccgctatccc
     4381 gagctggccc gcgtcgcgcg cgccggcatc gtgcaccggc tcgacaagga tacctccggc
     4441 ctgatggtgg tggcgcgcca cgacgcggcg cagacgcacc tggtgcggca attgcaggcc
     4501 cgcaccgtgg gccgcgaata cctcgcgctg gtgcatggct ggttgacggc ctccggcacg
     4561 gtcgaccggc ccatcggccg cgacgcccgg gtgccggtgc gcatgagcgt cgagcggccc
     4621 atcgccccca agccggccat cacccactac cggaccgacc ggctgggcgc ggtggcgggc
     4681 gacggtccgc atgccgcggc gtcgcccgtc acgcaggtga tctgccgcct ggaaacgggc
     4741 cgcacgcacc agatccgcgt gcatttgtcg agcctgggcc atccgctgct ggccgatgcg
     4801 ctgtacggcg gccgggcaat ggccggcgcg gcgcgccaga tgctgcatgc gcgggccttg
     4861 cgcttcgacg acccgggcgg cggcgaaccc ctgtctttcc agtcgtcccc gccggacgac
     4921 atgcttgctg tccaggaagc catacgatgg aattcatgag tgtcgaccag gtcgtccagg
     4981 gcctgcccgt ggtcaccggc gccgcgtggc cgggcgtgcg ctacttctgc accacgcgcg
     5041 ccggcggcgt gggcgtggcg ccgcacgaca cgctcaacct gggcctgcgc gccgacgacc
     5101 ggcccgaggc cgtcgccgag aaccgccgcc gcgtgcgcgc ggccgtgccg ggcgagccgc
     5161 tgtggctgcg ccaggtgcat ggcgcggcgg tgatggatgc ggatgcgccg gccgcgcccg
     5221 tcgagccggc tgccgatgcc agcgtgacgg cgcggcctgg ccaggtgctg gccatcatgg
     5281 cggccgactg cctgcccgtg gtcatcagcg atgcgcgcgg cagcgtgctg ggcgcggcgc
     5341 atgcgggatg gcgcggcctg gcgggcggcg tgctcgaaaa taccttgcgg gccatgcagg
     5401 ccaagcaacc cggggcgcag ggctggcgcg cctgggtggg cccgggcatc ggcccctcgg
     5461 ccttcgaggt gggcgaggat gtgttgcagg cctttgcccc ggacggcccc gaagccctgg
     5521 ccttgttcgc gccgcggccg ggcgtgccgg gcaagtggct ggccgacctg ccgggcctgg
     5581 cgcggctgcg cctgcggcgc gcgggggtcg cgcagccggt cctgagcggc ctgtgcaccg
     5641 tgagcgaggc aggacgtttc ttttcgtatc gccgcgaccg cgtcaccggg cgcatggcgt
     5701 tgctggcctg gctggcgtga cttccgtatt cccgccttga cagccatgcg cggggccatg
     5761 caacatcgaa ccatgctatt ccataacgat aactagaagg tgtggtgtga gcactccttt
     5821 gcatgctgtg tggcccggtc cggtaagcgt ggcgccggac gtcctggcgg agatccaggc
     5881 ggatttttca cgtgaatggc agcgtctttg cgatgaggcc gagcgcggcg tgctggctcc
     5941 gccgagagac cggcgctttg ccagcgtggc ctgggcttcc agcagccagc atgcgctgat
     6001 cgcccatgcc tatctgctgt cggcgcaggc catgagccgc atggtcgacg cggcccaggt
     6061 cagcgagccc ttgcgcgagc gcctgcgctt tgcgctgatg cagtggatcg acgccatgtc
     6121 gccggccaac ttcctggcct tcaatcccga ggttcaacag tccatcgtcg agagcgccgg
     6181 caagacgctc accatggggc tgggcaacct gctgcaggat gtgaaaaaag gccgcatcag
     6241 ccagacggac gaaacccaat tcgaagtggg ccgcaacctg gcgtgcacgc cgggccaggt
     6301 ggtgttcgag aaccgcctga tgcagctcat ccagtacgcg ccgcaaaccg gcacggtgta
     6361 ccagcggccg ctggtggtcg ttccccccaa catcaacaag tattacatcc tcgacctgca
     6421 gcccgacaat tccttcgtgc gccatgccgt cgagcagggg catacggtgt tcatcgtttc
     6481 gtggcgcaac ccgctggcga ccgacaccga cggcatcgac gcggccacct ggagcgatta
     6541 cctcgacgat gccgtgctca aggcgatctc ggtggccagc gagatcaccg gccagccgca
     6601 ggtcaatgcg ctgggcttct gcgtgggcgg caccttgctg gcctcggccc tggcgctggc
     6661 ccaggcgcgc ggcagcaacc cggccgcctc gctcacgctg ctgacctcgc tgctcgattt
     6721 ccacgacacc ggcgtgctca gcgtgttcgt cgacgaaggc cacgccctgc tgcgcgacca
     6781 gcagctgggc cacggcggcc tgatgttcgc gcgcgacctg gccaccacgt tctccttcct
     6841 gcgccccaac gaactggtgt ggaactacgt ggtgggcaac tatctcaagg ggcagacgcc
     6901 gccgcccttc gacctgcttt tctggaacgc cgacagcacc aatctgccgg ggcctttctt
     6961 tgcctggtac tttcgcaaca cctatctcga gaacaatctc aaggtgcccg gcaaggcgca
     7021 ggcctcgggg gtgccgctgg acctcacccg gctgacgatg ccggcctatc tgttcggttc
     7081 gcgcgaagac cacatcgtgc cctgggtatc ggcctatgcc tccacgcaat tgctgcgcgg
     7141 gcccatgcgc ttcgtgctgg gggcgtcggg gcacgtcgcc ggcgtggtca atccgcctgc
     7201 gcgcaacaag cgcagctatt gggtcgcccc gccggggcag gccgggctgc cgggcgaccc
     7261 gaacgcatgg ctgaaccatg ccgaggaaca tcccggcagc tggtggacgg actgggcgca
     7321 gtggctggcc ccgcacggcg gcaagcgggt gcgggcgccc gggcgcctgg gcggcgcaaa
     7381 ctatgaaccc atcgaagccg cgcccggtcg atacgtgaaa gtccgcgctg tgtagagtgc
     7441 ggcatactgc taaaaacgac atgagacatt tcgggcaatg cgcgtggcgc gccaggctcg
     7501 gacaacaagg agacaggaca tgagcagcgc aaaactggca tacgtgacgg gcggcatggg
     7561 cggcatcggc acttctatct gccaacgcct ggccaaggac ggatttcgcg tggtggccgg
     7621 ttgcggcccc agccgcaatt accagcaatg gctcgacgag caggccgcgc agggctacac
     7681 cttctattcc tcggtgggca acgtggccga ctgggaatcc accgtgctgg ccttcgagaa
     7741 ggtccgggcc gagtacgggg tggtcgacgt gctggtgaac aacgccggca tcacgcgcga
     7801 cggcctgttt cgcaagatga cggtcgacga ctggcgcgcc gtgatcgaca ccaacctcaa
     7861 cagcctgttc aacgtcacca agcaggtgat cgacggcatg gtcgacaagc agtgggggcg
     7921 catcatcagc atcagctcgg tcaacggcca gaagggccag ttcgggcaga ccaactattc
     7981 cacggccaag gcgggcatcc acggcttcac catggccctg gcccaggagg tcgccgccaa
     8041 gggcgtcacg gccaacacca tctcgccggg ctacatcggc accgacatgg tccgggccat
     8101 ccgccccgac gtgctcgaga agatcgtcgc caccattccg gtgcggcgcc tgggcatgcc
     8161 cgaggaaatc gcctccatcg tttcctggct ggcgtcggac gaatccggct tcgccaccgg
     8221 cgccgatttt tcgctcaacg gcggcctgca catgggctga ggcccctggc tgtacactcg
     8281 aaccattcca atcaatagac cgcgagatct aggggacaaa agaatgacgc aagcgcaaaa
     8341 aggcgccgcc cagcgcctga tcaaaaaata ccccaaccgc cggctgtacg acacgcagac
     8401 cagcacctac atcacgctga ccgatgtcaa gcagctcgtg cttgccaccg aggatttcca
     8461 ggtgatcgac gccaagagcg gcgatgacct gacccgcagc atcctgctgc agatcatcct
     8521 cgaagaggaa agcggcggca tgccgatgtt ctcctcgacc atgctggcgc agatcatccg
     8581 cttctacggc catgccatgc agggcatcat ggggtcctac ctcgagaaga acctccaggc
     8641 cttcatggag atccaggaac gcgtggccga gcagtccaag gggctgtatg gcaccaatct
     8701 cggccccgag gcctggacgc aattcatgaa cggccaggcg cccatgctgc agaccatgat
     8761 gaacaactac atcgaccaga gcaagaacct gttcgtgcag atgcaggacc agatgcagga
     8821 ccagacccgc aacatgtttt cgacctttcc cttcgtgccg ggaagcacgc cgggcgggcg
     8881 aaaataaagc aaggcgtcag tcatcagaaa gggggctgaa agcccccttt cctctttggt
     8941 aagcggctga aaccagcatg gccttgttgg cgtcaatgaa aacagttctt gtttttaatc
     9001 tctataatag aatttagaaa ctcactctgg tttcgttttc atgacaagga tgtcgttgat
     9061 gagcaagcgt agcctggccc tctcggcggg cctgttgggc ctgtccctgg tttttgccgg
     9121 cgccgcgggc gcggccgaat atccgatagg caagccggtc cagcaaggcg gcatggaagt
     9181 cggcgccgtc tacctgcagc ccatcgaaat ggatccgccc aacgtcatgc gcgcggccaa
     9241 ggattccgac atccacctcg aggccgacat ccacgccatt gccgacaaca agaccggctt
     9301 tcccgaaggc gagtggatgc cttacctggt ggtccactac gaattgcaga aggccggcag
     9361 cgccaatgtg gtcaagggca tgctcatgcc catggtggcc aacgacggtc cgcactatgg
     9421 cgacaacgtc aagctcgaag gccccggcaa gtatcacctc aagctgtcgg tgcagccgcc
     9481 cacggccgaa cccatgaatc acttcggccg ccacatcgac aaggaaaccg gcgtgggccc
     9541 gtggttcgag ccgctcaatc tcgactatga cttcgccttc gccggcaccg gcaagaaggg
     9601 cgggtattga tcatgcggcg tgggcttgca gggctgttgc tggcg
//
LOCUS       DASWPO010000112        15036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_470694, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000112 DASWPO010000000
VERSION     DASWPO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15036
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470694"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VGC69_06490"
     CDS             <1..709
                     /locus_tag="VGC69_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0294967.1"
                     /translation="RQELQMGADQIKIMASGGVASPTDPIASFGYSEDEVRAIVDEAA
                     SRQTYVMAHSYTAAAIERVVRLGVRTIEHGNLIDEPVARLMAEKGAYVVPTLVTYEAL
                     AEEGASYGLPPDSVAKIATVREAGLRSLEIYRKAGVKMGYGSDLLGPSQRLQSDEFRI
                     RAQVLSAQEVIQSATTVAAEVLRMEGKLGRLEAGAIADALVVDGNPYRDLSCLLGQGE
                     GIALVMKGGRIHHNELK"
     gene            722..4540
                     /gene="putA"
                     /locus_tag="VGC69_06495"
     CDS             722..4540
                     /gene="putA"
                     /locus_tag="VGC69_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0294968.1"
                     /translation="MASTTLGVKVDDMLRDRLKAAAQKLNRTPHWLHKQALLAYIEKI
                     ERGQLPAEVSYFSSDAADGGEGDAASGAVTPPFFEFGQDVQPQSVLRAAITAAYRRPE
                     PECVPLLLGQARAPNAEKVHAMAKRLILALRGKHKGGGVEGLLHEFSLSSQEGVALMC
                     LAEALLRIPDRATRDALIRDKIAHGDWKSHTGGRQSLFVNAATWGLMLTGKLVAVNSE
                     QGLSAALTRLIGKGGEPLIRQGVNMAMRMMGEQFVSGQTIAEALANNRKLEARGFRHS
                     YDMLGEAATTAADADRYYADYEQAIHAIGKASAGRGIYEGPGISIKLSALHPRYSRSQ
                     RERVMQQLLPRIEALAILARGYDIGLNIDAEEADRLELSLDLLEALCHTPQLEGWNGI
                     GFVIQAYQKRAPFVIDFVIDLARRSHHRLMVRLVKGAYWDTEIKRAQIDGLEGYPVYT
                     RKVYTDVAYVACARKLLGAPEAIYPQFATHNAYTLSAIYHLAGQNYYPGQYEFQCLHG
                     MGESLYEEVVGPVMHGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSFVNQIGDA
                     SIPVEKLAVDPVEAAARIAPLGAPHEKIPLPRDLFGAAGPGARVNSAGLDLSNEHRLG
                     SLAAALLAGAAADSRAAPMLDEALPWDEARAVEVRNPADRRDLVGKVMEAGEAEVAAA
                     LRAAQKSVSIWQSTPVGERANCLLRAAQLLEERMQGLLGLIVREAGKSLPNAIAEVRE
                     AVDFLRYYAGQAQREFDNDTHRPLGTVLCISPWNFPLSIFAGQVGAALAAGNTVIAKP
                     AEQTPLIAAQMIALLREAGVPAGAVQLLPGRGETVGAALVASAAVHGVLFTGSTEVAR
                     VIARTLAERLDDAGRVIPLIAETGGQNAMVVDSSALAEQVVADVLGSAYDSAGQRCSA
                     LRVLCVQDDCADHVLAMLRGAMRELQVGTPDLLGTDVGPVIDAQASGVIQRHIDAMRE
                     AGHPIDQVEMDARCRYGTFVAPTLIEIDSLAELKREVFGPVLHVMRYARDRLDDLIDQ
                     INGTGYGLTFGVHTRIDETMRRVTERVRAGNLYVNRNIVGAVVGVQPFGGEGLSGTGP
                     KAGGPLYLYRLLSVRPSASAGLVDGVARSPAGQVLPGPTGELNIYRLLPRGAVLGIAA
                     TELGMQAQRSAAHGTGNALLLWDTPMARAWLAGQDPAVRAGVSLVADGGLDQADFDAV
                     LFEGDSDALLEWNRRIAARPGPILTVQGLTTDALAAGQGYALDRLLAEKSLSINTAAA
                     GGNASLMTIG"
     gene            4573..6186
                     /locus_tag="VGC69_06500"
     CDS             4573..6186
                     /locus_tag="VGC69_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0294969.1"
                     /translation="MSSDNPYAQAEVGEETIAMNLAKGFKPPVAATSRDRGEGPFTRL
                     VLRGAIIIDGTGAPPWGPVDIVVENDRIAAIVNVGTPHKPINPDRRPAAGDYEIDCHG
                     KYVTPGLIDTHGHIGTPYHAMSGVVPPADYIYKLWLAHGVTTVREMGGMNGLNWTLDQ
                     KNLAEANQIAAPHLVIHTVFPAANDRVKTIHTPAQAREWVRKLKARGADGIKFFGAPP
                     AIMQAALDEAAQVGLRSGCHHAQMAVTRVNALKSAVWGLTSSEHYYGLPEALFENRVV
                     QDYPTDYNFSDEYYRFSLSGQTFAQAAKPGSAKWREVMDKFLEVGHTFVPTFNIYDAN
                     RDLMRARRADWHDEYTWKAIWKYFQPQRGGHGAYWYRWSTTNEIEWKQNYQLWMQFIN
                     EYKNLGGRVCAGSDSGFIYQIFGFGLIRELELLQEAGFHPLEVLRAATISGAELLGIE
                     EEAGSVDVGKRADILIHDQNPLEDFKLLYGTGAMRLNDDNGQVQWKRALRTTIKGGVV
                     YDTEQLLADVRELVAQSWEGDPDGPPHRR"
     gene            6164..7186
                     /locus_tag="VGC69_06505"
     CDS             6164..7186
                     /locus_tag="VGC69_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0294970.1"
                     /translation="MAPRTGAEPPAPPVHLIILTGFLGSGKTSLLVDFLRSDQAGDTG
                     VIVNEAGEIGIDGAIVADGASGVPMTLLSNGCVCCSLRSGLVDTVNALLEAPRPPGRG
                     PLTRIVLETSGLSRPGPIIASLADPELSRRNLRLAVLASYDCEADAARDTQFEEAAAQ
                     FAAAQRIVLTKVDRIEPGSLALHRARAQGLGPLAQVVAEAGRAEAVRQAFAPLPGAQA
                     GDLALQALFSAGQGAGSLQHPRIRVWTGTPRAPLDWDDFSMWLDDLAGFCGERLLRVK
                     ALLKVNDCDEPILIQSVGTTFGMPRRMAQAQAGKDVLVVITRDLELADLQAMAPDGPV
                     RLAALA"
     gene            7271..8338
                     /locus_tag="VGC69_06510"
     CDS             7271..8338
                     /locus_tag="VGC69_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0294971.1"
                     /translation="MNSRGMYRRDFLKTAAGLGLASTLPAMPGLARAAGGDVVVGTWG
                     GDYQNLLQQIISPLLEKQGVGVTFDTGNSVPRITKLRAEKNARRGSMDVPLLSDLDMY
                     EVSLADVLAPVQANLSHLPNLANVYDQFKTPYSIPHIFSAMVLVYSTEKFPTPPTSIE
                     VMLDPKNKGGVGFSDILFSPNALFVGAGGSAPGRLSSFEPGKKFLTELKKNAPRVYPS
                     NEAVATAFKSGEIWVSAMWKARALQWRDAGLPLDFVIPKEGSIPVTFEAAVAKNTRNP
                     ESAWAYLNAMLDPAGQVLFAKAMGYAPTVRNAPLPDDLRKRVGFTDDEIKGLRPYDLA
                     GLNDSKADFIEFWNKSFKAGL"
     gene            8384..9529
                     /locus_tag="VGC69_06515"
     CDS             8384..9529
                     /locus_tag="VGC69_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0294972.1"
                     /translation="MSPSLRGLFIASAAAAGLAAGAAQAADVKLGFAAPLTGPQAHYG
                     IDMQNGLQMALDEANKQGIKLDGDTAHFVLVSRDDQADPKTGVVVAQELVDEHVQGIL
                     GHFNSGTSIPASRVYHDAHLPEIAMATSPAFTAQGYDTTFRMMTSDTQQGAADGKFMV
                     SVLKARKVAIIDDRTAYGQGLADEVEKAVKAAGGQVVRREFTTDKSTDFTSILTNIKK
                     AAPTAVFFGGVDAQSAPMKQQMAKLGIKAPLVSGEMTRSDDFIKIGGKATEGTYASLA
                     GVPLETMVAGKGFAQRYEASFKQAPGVYSPYAYDGAWNMIAAMQKAGSADPAKYLPEL
                     ARLARKGATSGHIAYDAKGDLKEISVTIYQVRGGKWEMVQTMVSQAD"
     gene            9730..11127
                     /gene="mdtD"
                     /locus_tag="VGC69_06520"
     CDS             9730..11127
                     /gene="mdtD"
                     /locus_tag="VGC69_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HEY0294973.1"
                     /translation="MSPKHTSHDALLWIVATGFFMQTLDTTIINTALPSIARYLGETP
                     LAMQPVVVAYTLTMAILTPASGWLADRFGTRRVYFAAILVFVIGSLCCSFSHTLGQLT
                     AARVLQGVGGSMLLPIGRLAILRSVPGEQYVAALTFASIAGQVGPIVGPTLGGWFVEA
                     ASWHWIFLVNVPIGIVGLFAVRRYLPEGGHGSAPRFDVVGCSLLSLCMAAFSLAVDAP
                     SQSHRVAGSAALFALSLFTALAYIPHARRQREPLFRLGLFREPNFSVGLAGNLVARVG
                     ASSVPFLVPLLMQMQLGYSPIRSGMMMLPAALAGVLTKRCIAPLLQHFGHRAFLLANT
                     LIVGVSIMAFAMVSLASPPIVEIVILAIFGGANSMQFAAMNSVTLSGLPRRDAGSGNS
                     LFSMVQMLAMGLGVSIGGGLVQAFAAHGGSAQGGFKFSFVCVGAITLLSALLFRHIDN
                     APAPAAAAEPARAGR"
     gene            complement(11160..12464)
                     /locus_tag="VGC69_06525"
     CDS             complement(11160..12464)
                     /locus_tag="VGC69_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294974.1"
                     /translation="MSSPPTPARNASPFPRLGQRYAFVVVGVIFFCLLFSAGLRSTPS
                     VLLTPLQQTFGWSRSIISLAAAIGIFFYGMMGPFAAAAMERFGLRHILLTALVVMAAS
                     CAASAYMTQTWHLIALWGIFSGMASGAVAVVLGATVVNRWFVKHRGLAMGVLTASTAT
                     GNLIFLPVLASLASSGDWTRAVWAVAIAALVMVPLVWWLVPGRPADVGLLPYGSDPAD
                     PPKAAAPRTGLLTATFGALKRAVRTRAFWYLFATFWVCGFTTNGLVGTHLIALCGDHG
                     IAPVEAAGLLSMMGVFDLIGTTASGWLTDRYDPRKLLFVYYALRGVSLIVLPYTDFSF
                     YSLSIFAVFYGLDWIATVPPTLRLANEAFGERDAPVVFGWIVAGHQIGAATAAWMGGF
                     VREATGSYLMAFVLSGATGIIAAVIALLVKRQRATPLEPAVA"
     gene            12599..13219
                     /locus_tag="VGC69_06530"
     CDS             12599..13219
                     /locus_tag="VGC69_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0294975.1"
                     /translation="MTKARQAAARPETQGSTTEPLRAADRIRRSARDLFYHQGIRAVG
                     VDAIVAEAGVTKPSLYRNFASKDELAAAYLHDYETVFWSRFDAAVAAHPGDPRAQILA
                     FFEGLADRVANMPGYRGCGLTNAAIEYPEPDHPARLVSVANKQQVRERLIDMARRMGA
                     RDPQVLGDGLQLLMEGAYASSQLLRVGGPVQHLPVVAARLIDASLQ"
     gene            13478..14470
                     /locus_tag="VGC69_06535"
     CDS             13478..14470
                     /locus_tag="VGC69_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0294976.1"
                     /translation="MKSVLRRLGAMLSLSVAAVALCLSAPARAVEISVTQWGASLYGL
                     PYAVAMDQGLFKKAGVDITSILSSGGGGTTVRNILASDVPYGEVAVSAALAAQRQGLD
                     LVIVNVGTRSVAEASMVTMPNSDVKDVADLVGKKIAITSPKSVSEMLFLMTLKEKGID
                     AGKVQRVASGGYVNGLTLLEQGGVSAAVLIEPLSIVRKAQYRTVYHAGEILKPMTTSV
                     GITTRKFAAEHPDVLKAIIAGRRAGVEATYADPGAAAKLLESSFKLTPEVAREAVDNM
                     VKSRMWSDGEFDRAELDRMVAGLKIIGELNEDVDWSKLVDTAFLPADLQAKSKL"
     gene            14467..>15036
                     /locus_tag="VGC69_06540"
     CDS             14467..>15036
                     /locus_tag="VGC69_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0294977.1"
                     /translation="MSNVAPLIKPVAKPDAPAAASAAQREAHAVLSGVTRYFDKAGPR
                     ERFHALGPIDLTLHQGEFFSVVGPSGCGKSTLLDILAGLAAPTSGAVLFEGREVKGVP
                     DGVGVVFQEDASFPWLTVRDNIGFGLRMAGMDGAEIKRRVDYALGFMGLKDFASAYPV
                     QLSGGMRQRVCIARTLVQQPRLILLDEPFG"
BASE COUNT         2462 a         5112 c         5151 g         2311 t
ORIGIN      
        1 gcgccaggaa ctgcagatgg gcgcggacca gatcaagatc atggcctccg gcggcgtggc
       61 ctcgccgacc gacccgatcg cctccttcgg ctattccgag gacgaggtcc gcgccatcgt
      121 cgatgaggcc gcgtcccggc agacctacgt catggcgcac tcgtacaccg ccgccgccat
      181 cgagcgcgtg gtgcgcctgg gcgtgcggac catcgagcac ggcaacctga tcgacgagcc
      241 cgtggcccgc ttgatggccg agaagggcgc ctacgtggtg ccgaccctgg tgacctacga
      301 agccctggcc gaggagggcg ccagctacgg cctgccgccc gacagcgtcg ccaagatcgc
      361 caccgtgcgc gaggccggcc tgcgttccct ggaaatctac cggaaggccg gcgtgaagat
      421 gggctacggc tcggacctgc tggggccgtc gcagcgcctg caaagcgacg aattccgcat
      481 ccgcgcccag gtgttgtccg cgcaggaggt catccagagc gccaccaccg tggccgccga
      541 ggtgctgcgc atggaaggca agctgggccg cctggaggcg ggcgcgatcg ccgacgcgct
      601 ggtggtcgac ggcaacccct accgcgacct gtcctgcctg ctgggccagg gcgagggcat
      661 tgccctggtg atgaagggcg gccgcatcca tcacaacgaa ctgaagtgac gagtattcgc
      721 catggctagc acgaccctgg gcgtcaaagt ggatgacatg ctgcgcgacc ggctcaaggc
      781 cgccgcgcag aagctcaacc gcacgccgca ttggctgcac aaacaggcgc tgctggctta
      841 tatcgagaag atcgagcgag gccagctgcc ggccgaggtg tcctatttct cgtccgacgc
      901 cgcggacggc ggcgagggcg atgccgcgtc gggcgcggtg acgccgccct tcttcgagtt
      961 cggccaggac gtgcagccgc agtccgtgct gcgcgcggcc atcacggcgg cctaccggcg
     1021 ccccgagccc gagtgcgtgc cgctgctgct gggccaggcc cgggcgccca acgccgaaaa
     1081 agtccacgcc atggccaagc gcctgatcct ggccctgcgc ggcaagcaca agggcggcgg
     1141 ggtcgagggc ctgctgcatg agttctcgct gtcgagccag gaaggcgtgg cgctgatgtg
     1201 cctggccgag gccctgctgc gcatcccgga ccgggccacg cgcgacgccc tgatccgcga
     1261 caagatcgcc cacggcgact ggaagtcgca cacgggcggg cgccagtcgc tgttcgtcaa
     1321 cgcggccacc tggggcctga tgctgaccgg caagctggtg gcggtcaaca gcgagcaagg
     1381 cctgtcggcc gcgctgacgc gcctgatcgg caagggcggc gagccgctca tccgccaggg
     1441 cgtgaacatg gccatgcgca tgatgggcga gcagttcgtc tccggccaga ccatcgccga
     1501 ggcgctggcc aacaaccgca agctggaggc ccgcggcttt cgccattcgt acgacatgct
     1561 gggcgaggcg gccaccaccg ctgcggacgc cgaccgctac tacgccgact acgagcaggc
     1621 catccatgcc atcggcaagg cgtcggccgg ccgcggcatc tacgaagggc cgggcatctc
     1681 gatcaagctg tcggcgctgc atccgcgcta ttcgcgctcg cagcgcgagc gggtgatgca
     1741 gcaactgctg ccgcggatcg aggcgctggc catcctggcg cggggctacg acatcggctt
     1801 gaacatcgac gccgaagagg cggaccggct cgagctgtcc ctcgacctgc tcgaggccct
     1861 gtgccatacc ccgcagctgg aaggctggaa cggcatcggc ttcgtgatcc aggcctacca
     1921 gaagcgcgcg cccttcgtga tcgacttcgt catcgacctg gcgcgccgca gccaccatcg
     1981 cttgatggtg cggctggtca agggcgccta ctgggacacc gagatcaagc gcgcccagat
     2041 cgacggtctg gagggctatc cggtgtacac ccgcaaggtc tacaccgacg tggcctacgt
     2101 ggcctgcgcc cgcaagctgc tgggcgcgcc ggaggcgatc tacccgcagt tcgccacgca
     2161 caatgcctac accctgtcgg ccatctacca cctggccggg cagaactact accccggcca
     2221 gtacgaattc cagtgcctgc acggcatggg cgaatcgttg tacgaagaag tggtcggccc
     2281 ggtgatgcac ggcaagctca accggccctg ccgcatctat gcgccggtgg gcacgcacga
     2341 gaccctgctg gcctacctgg tgcggcgcct gctggaaaac ggcgccaaca cgtcgttcgt
     2401 gaaccagatc ggcgacgcgt ccatcccggt ggagaagctg gcggtcgacc cggtcgaagc
     2461 ggccgcgcgg atcgcgccgc tgggcgcgcc gcacgagaag atcccgctgc cccgcgatct
     2521 gttcggcgcc gcgggcccgg gcgcccgggt caactcggcc gggctggacc tgagcaacga
     2581 acaccggctg ggctcgctcg ccgcggccct gctggccggc gccgcggccg atagccgcgc
     2641 ggctcccatg ctggacgagg ccttgccctg ggacgaggcg cgcgccgtcg aggtgcgcaa
     2701 cccggcggac cggcgcgacc tggtgggcaa ggtcatggag gcgggcgaag ccgaggtcgc
     2761 cgcggcgctg cgcgccgcgc agaagtcggt gtcgatctgg cagtccacgc cggtcggcga
     2821 gcgcgccaat tgcctgctgc gcgccgccca gctgctcgaa gagcgcatgc aggggctctt
     2881 gggcctgatc gtccgcgagg ccggcaagtc gctgcccaat gccattgccg aggtgcgcga
     2941 ggcggtcgac ttcctgcgct attacgcggg gcaggcgcag cgcgagttcg acaacgacac
     3001 gcaccggccg ctgggcacgg tgttgtgcat cagcccctgg aatttcccgc tgtcgatctt
     3061 cgccggacag gtgggcgccg ccctggccgc cggcaatacc gtcatcgcca agccggctga
     3121 acagacgccc ctgatcgccg cgcagatgat cgccctgctg cgcgaggccg gcgtgccggc
     3181 cggcgccgtg cagctgctgc cgggccgcgg cgagaccgtg ggcgccgcgc tggtcgcgag
     3241 cgcggcggtg cacggcgtgc tgttcacggg ttcgaccgag gtggcgcggg tgatcgcccg
     3301 cacgctggcc gaacggctcg acgatgccgg gcgcgtcatt cccctcatcg ccgagacagg
     3361 cgggcagaac gccatggtcg tcgattcgtc ggcgctggcc gaacaggtcg tggccgacgt
     3421 gctgggctcg gcctacgact cggccggcca gcgctgctcg gcgctgcgcg tgctgtgcgt
     3481 ccaggacgac tgcgcggatc acgtgctggc catgctgcgc ggcgccatgc gcgaattgca
     3541 ggtcggcacc ccggacctgc tcggcacgga cgtggggccg gtgatcgacg cgcaggccag
     3601 cggcgtgatc cagcggcaca tcgatgccat gcgcgaggcc ggccacccga tcgaccaggt
     3661 cgagatggac gcccgctgcc ggtacggcac cttcgtcgcg cccaccctca tcgagatcga
     3721 cagcctggcc gagctcaagc gcgaggtgtt cggcccggtg ctgcacgtca tgcgctatgc
     3781 gcgcgaccga ctggacgatc tgatcgacca gatcaacggc acgggctatg gcctgacctt
     3841 cggcgtgcac acccgcatcg acgagacgat gcggcgcgtg accgagcgcg tgcgcgccgg
     3901 caacctctac gtcaaccgca atatcgtcgg cgccgtggtg ggcgtgcagc ccttcggcgg
     3961 cgaagggctc tccggcacgg ggcccaaggc cggcggaccg ctgtacctgt accgcctcct
     4021 gtcggtgcgg ccgtccgcgt cggccggcct ggtggacggc gtggcgcgca gccccgccgg
     4081 gcaggtgctg ccggggccga cgggcgagct caacatctac cggctgctgc cgcgcggcgc
     4141 ggtgctgggc atcgccgcga ccgagctggg catgcaggcg cagcgcagcg cggcccacgg
     4201 cacgggcaac gccctgctgc tgtgggacac gcccatggcg cgcgcctggc tggccgggca
     4261 ggacccggcc gtgcgggccg gcgtgtccct ggtcgcggac ggcggcctgg accaggcgga
     4321 tttcgatgcg gtactgttcg agggcgacag cgacgccttg ttggaatgga accgccgcat
     4381 cgcggcgcgg ccaggaccga tcctcaccgt gcaggggctg acgacggatg cgctggcggc
     4441 cgggcaaggc tatgcgctcg accgattgct ggccgagaag tccctgagca tcaatacggc
     4501 cgccgccggc ggcaatgcca gcctgatgac catcggctga gcccttcacg acaattcaga
     4561 caggagcgtt tcatgtccag cgacaaccct tatgcccagg ccgaggtcgg cgaagaaacc
     4621 atcgccatga acctggccaa gggcttcaag ccgccggtgg ccgccacctc gcgcgatcgg
     4681 ggcgaggggc ccttcacgcg cctggtgctg cgcggcgcca tcatcatcga cggcacgggc
     4741 gcgccgccct gggggccggt ggacatcgtc gtcgagaacg accgcatcgc cgccatcgtc
     4801 aacgtcggca cgccgcacaa gcccatcaac cccgaccgcc ggcccgcggc cggcgactat
     4861 gaaatcgact gccacggcaa gtacgtcacg cccggcctga tcgacacgca cggccacatc
     4921 ggcacgcctt accacgccat gagcggcgtg gtgccgccgg ccgactacat ctacaagctc
     4981 tggctggccc acggcgtgac caccgtgcgc gaaatgggcg gcatgaacgg cctgaactgg
     5041 acgctcgacc agaagaacct ggccgaggcc aaccagatcg ccgcgccgca cctggtcatc
     5101 cataccgtct ttccggcggc caatgaccgc gtcaagacca tccacacgcc cgcccaggcg
     5161 cgcgagtggg tgcgcaagct caaggcgcgc ggcgccgacg gcatcaagtt cttcggcgcg
     5221 ccgccggcca tcatgcaggc cgcgctggac gaggccgccc aggtcggcct gcgttcgggc
     5281 tgccaccatg cccagatggc ggtgacgcgc gtcaacgcgc tgaaatccgc cgtgtggggc
     5341 ttgacgagtt ccgagcatta ttacggcctg cccgaagcgc tgttcgagaa ccgcgtggtg
     5401 caggactatc ccaccgacta caacttcagc gacgagtact accgcttctc cctgtccggg
     5461 cagaccttcg cgcaggccgc caagccgggc agcgcgaagt ggcgcgaagt catggacaag
     5521 ttcctggagg tcggccacac cttcgtgccc accttcaaca tctacgacgc caaccgcgac
     5581 ctgatgcgcg cgcgccgggc cgactggcac gacgaataca cctggaaggc catctggaag
     5641 tatttccagc cgcagcgcgg cggccacggc gcctactggt atcgctggag caccaccaac
     5701 gaaatcgagt ggaagcagaa ctaccagctg tggatgcagt tcatcaacga atacaagaac
     5761 ctgggcgggc gcgtatgcgc gggcagcgac tcgggcttca tctaccagat cttcggcttc
     5821 ggcctgatcc gcgagctcga gctcttgcag gaggcgggct tccatccgct ggaggtgctg
     5881 cgcgcggcca ccatctcggg cgccgagctg ttgggcatcg aggaggaggc gggctcggtc
     5941 gacgtgggca agcgcgccga catcctcatc cacgaccaga acccgctgga agatttcaag
     6001 ctgctctacg gcaccggcgc catgcgcctg aacgacgaca acggccaggt gcagtggaag
     6061 cgcgcgctgc gcaccaccat caagggcggc gtggtgtacg acaccgagca acttctggcc
     6121 gacgtccgcg aactcgtcgc gcaaagctgg gaaggagacc ccgatggccc cccgcaccgg
     6181 cgctgaaccg ccggctccgc cggtccacct gatcatcctc acggggttcc tgggcagcgg
     6241 caagaccagc ctgctggtgg acttcctgcg cagcgaccag gcgggcgata ccggcgtgat
     6301 cgtcaacgag gccggcgaga tcggcatcga cggcgccatc gtggccgacg gcgcgagcgg
     6361 cgtgccgatg acgctgctgt ccaacggctg cgtctgctgt tcgctgcgca gcggcctggt
     6421 cgatacggtc aacgccctgc tggaggcgcc gcgcccgccg ggccgcggtc ccttgacgcg
     6481 catcgtcctg gaaaccagcg gcctgtcgcg gccggggccc atcatcgcct cgctggccga
     6541 cccggagctg tcccggcgca acctgcgcct ggcggtgctg gcgagctacg actgcgaggc
     6601 cgacgccgcg cgcgacacgc agttcgagga agccgccgcc cagttcgcgg cggcgcagcg
     6661 catcgtgctg accaaggtgg ataggatcga gcctggctcc ctggcgctgc atcgcgcccg
     6721 ggcgcagggc ttgggccccc tggcgcaggt ggtggccgag gccggccgcg ccgaggccgt
     6781 gcggcaggcg ttcgcgccct tgcccggcgc gcaggcgggc gacctggcgt tgcaggcctt
     6841 gttcagcgcg gggcagggcg ccggctcgct gcagcatccc cgcatccgcg tctggacggg
     6901 aacgccgcgc gcaccgctgg actgggacga tttttccatg tggctcgacg acctggccgg
     6961 cttctgcggt gaacggctgc tgcgggtcaa ggcgctcctg aaagtgaacg actgcgacga
     7021 gcccatcctg atccagagcg tgggcaccac cttcggcatg ccccgccgca tggcgcaggc
     7081 gcaggccggg aaagacgtgc tggtcgtgat cacccgcgac ctggagcttg ccgatttgca
     7141 ggccatggcg ccggacggcc ccgtccgtct cgcagccctg gcctgaacgc agtgcaatga
     7201 agtcgcagtc ccgtcatcat gcggcccatg ccgcgttatt ccgaacctac gcaattacaa
     7261 gaggaaagcc atgaattcaa ggggtatgta tcgccgtgat tttctgaaga ccgccgccgg
     7321 ccttgggctg gcgtcgacgc tgcccgccat gccgggcctg gcccgcgcgg ccgggggcga
     7381 cgtcgtggtc ggcacctggg gcggggatta ccagaatctc ctgcagcaga tcatctcgcc
     7441 gctgctcgaa aagcagggcg tgggcgtgac tttcgacacc ggcaactcgg tgccgcgcat
     7501 caccaagctg cgggccgaga agaacgcccg ccgcggcagc atggacgtgc cgctcctgag
     7561 cgacctggac atgtacgaag tctcgctggc cgacgtgctc gcgccggtgc aggccaatct
     7621 cagccatctg ccgaacctgg ccaacgtcta cgaccagttc aagacgccgt actcgatccc
     7681 gcacatcttc agcgccatgg tgctggtcta cagcaccgag aagttcccga cgccgcccac
     7741 ctcgatcgag gtgatgctcg acccgaagaa caagggcggc gtgggcttct ccgacattct
     7801 cttctcgccc aatgccttgt tcgtgggtgc gggcggcagc gcccccggcc gcctgagcag
     7861 cttcgagccc ggcaagaaat tcctgaccga gctcaagaag aacgcgccgc gcgtctaccc
     7921 ctccaacgag gcggtcgcca cggccttcaa gtcgggcgag atctgggtct cggccatgtg
     7981 gaaggcgcgc gccctgcagt ggcgcgacgc gggcctgccg ctggatttcg tgattcccaa
     8041 ggaaggctcg atcccggtga ccttcgaggc ggccgtggcc aagaacacgc gcaaccccga
     8101 atcggcctgg gcctacctga atgcgatgct cgatcccgcc ggccaggtgc tgttcgccaa
     8161 ggccatgggc tatgcgccca ccgtgcgcaa cgcgccgctg ccggatgacc tgcgcaagcg
     8221 cgtgggcttt accgatgacg agatcaaggg cttgcgtccc tacgacctgg cggggttgaa
     8281 cgacagcaag gccgatttca tcgagttctg gaacaagtcg ttcaaggcgg gtctgtaagc
     8341 cgccatcccc ggatattctc cgaaccgaat agaggagctt cctatgtccc catctcttcg
     8401 cggtctgttc atcgcctcgg cggcggctgc cggcctggcc gccggcgccg cgcaggcggc
     8461 cgacgtcaag ctcggtttcg cggcgccgct caccggcccg caggcccact acggcatcga
     8521 tatgcaaaac ggcctgcaga tggcgctgga cgaggccaac aagcagggca tcaagctcga
     8581 cggcgatacg gcccacttcg ttctggtgtc gcgcgatgac caggccgatc ccaagacggg
     8641 cgtggtggtg gcccaggaac tggtcgacga gcatgtccag ggcatcctgg gccacttcaa
     8701 ctcgggcacc tcgatcccgg cttcgcgcgt ctaccatgac gcgcacctgc ccgagatcgc
     8761 catggccact tcgccggcct tcaccgcgca gggctacgac accacctttc gcatgatgac
     8821 cagcgacacc cagcagggcg cggcggacgg caagttcatg gtgagcgtgc tcaaggccag
     8881 gaaggtcgcc atcatcgacg accgcacggc ctacggccag ggcctggccg acgaggtgga
     8941 gaaggccgtg aaggccgccg gcggccaggt ggtgcggcgt gagttcacca cggacaagtc
     9001 caccgacttc acgtccatcc tcaccaacat caagaaggcc gcgccaactg ccgtgttctt
     9061 cggcggcgtg gacgcccagt ccgcgcccat gaagcagcag atggccaagc tgggcatcaa
     9121 ggcgccgctg gtgtcgggcg aaatgacccg cagcgacgac ttcatcaaga tcggcggtaa
     9181 ggcgaccgaa ggcacctatg cctcgctggc cggcgtgccg ctggagacca tggtggctgg
     9241 caagggcttc gcgcagcgct atgaagccag cttcaagcag gctccgggcg tctattcgcc
     9301 ctatgcctat gacggcgcgt ggaacatgat cgcggccatg cagaaggccg gctcggccga
     9361 cccggccaag tacctgcccg aactggccag gctcgcgcgc aagggcgcga ccagcggcca
     9421 catcgcctac gacgccaagg gcgacctgaa ggaaatctcg gtcaccatct accaggtgcg
     9481 cggcggcaaa tgggaaatgg tgcagaccat ggtgagccag gccgactgac cattcgatgc
     9541 ccatgatgcc caagggccgt tcgcgagaac ggccctttgc gtttggacgg ctcggcagct
     9601 gacggttgat tgaatctctg tccagccagt ttggttctat gattgcaggt tttcgatgtt
     9661 ttcgtcggcc gcgcgggcgg ccgccatacg accgccatgc ggccggcttg ctgtttgccg
     9721 aattccacaa tgtctcccaa acatacttcc cacgacgcgc tgttgtggat cgtcgctacc
     9781 ggctttttca tgcagacgct ggacacgacg atcatcaaca ccgcattgcc gtccatcgcc
     9841 cgctatctgg gcgagacgcc gctggccatg cagccggtag tggtcgccta tacgctgacg
     9901 atggcgatac tgacccccgc atcgggttgg ctggccgacc ggttcggcac gcggcgcgtc
     9961 tatttcgccg ccatcctggt gttcgtgatc ggctcgctgt gctgctcctt ctcgcatacg
    10021 ctgggccaat tgaccgccgc gcgcgtgctg caaggcgtgg gcggctcgat gctgctgccg
    10081 atcggcaggc tggcgatcct gcgcagcgtg ccgggcgagc aatacgtggc cgcgctgacg
    10141 tttgcctcga tcgccgggca ggtcggcccg attgtcggac cgacgctcgg cggctggttc
    10201 gtcgaagccg cgtcgtggca ttggatcttc ctggtcaacg tgccgatcgg catcgtgggc
    10261 ttgttcgcgg tgcgccgcta tctgcccgaa ggcggccacg gtagcgcacc gcgcttcgac
    10321 gtggtgggct gcagcttgtt gtcgctgtgc atggcggcct tctcgctggc ggtcgacgca
    10381 ccgtcgcaaa gccatcgcgt cgcagggtcg gcggccctgt tcgcgctgag cctgttcacc
    10441 gcgctggcct atattccgca cgcgcggcgc cagcgcgagc cgctgtttcg gctcggcttg
    10501 ttccgcgaac ccaacttcag cgtcggcctg gccggcaacc tggtggcccg cgtgggcgcc
    10561 agctcggtgc cctttctggt gcctttgctg atgcagatgc agctgggcta ctcgcctatc
    10621 cgctccggca tgatgatgct gccggccgcg ctggccggcg ttttgaccaa gcgctgcatc
    10681 gcgccgctgc tccagcactt cggccatcgc gcgtttctgc tcgccaatac gctgatcgtc
    10741 ggcgtgtcga tcatggcgtt cgccatggtg tcgcttgcct cgccgccgat cgttgaaatc
    10801 gtcattctcg cgattttcgg cggcgccaac tcgatgcagt tcgccgcgat gaacagcgtc
    10861 acgctcagcg gcctgccgcg ccgggatgcc ggcagcggca atagcctgtt ctcgatggtc
    10921 cagatgctgg cgatggggct gggcgtgtcc atcggcggcg gcctggtgca ggcgttcgcc
    10981 gcgcacgggg gctcggcgca aggcggcttc aagttcagct tcgtctgcgt cggcgccatc
    11041 acgctgctgt cggcactgct gtttcgccac atcgacaacg cgcccgcgcc cgcggcggct
    11101 gccgaaccgg cgcgggccgg ccggtagcgg cggctggcgc gcctgcgggc tattttccgt
    11161 caggccacgg ccggctccag gggcgtggcg cgttgccgct tgaccagcag cgcgatcacg
    11221 gcggcaatga tgccggtggc gcccgacagc acgaaggcca tcaggtagct gccggtggct
    11281 tcgcgcacga agccgcccat ccaggccgcc gtggcggcgc cgatctggtg gccggcgacg
    11341 atccagccga acaccacggg cgcgtcgcgt tcgccgaagg cttcgttggc caggcgcagc
    11401 gtgggcggca cggtggcgat ccagtccagt ccgtagaaca cggcgaagat ggacaggctg
    11461 tagaacgaga agtcggtgta gggcaggacg atcagcgaca cgccgcgcag ggcgtagtag
    11521 acgaaaagca gcttgcgcgg atcgtagcgg tcggtgagcc agccggaggc cgtggtgccg
    11581 atcaggtcga acacgcccat catcgacagc aggccggcgg cctcgaccgg ggcgatgccg
    11641 tggtcgccgc acagcgcgat gaggtgcgtg cccaccaggc cgttggtggt gaagccgcac
    11701 acccagaagg tggcgaacag ataccagaag gcgcgtgtgc ggacggcgcg cttgagcgcg
    11761 ccgaaggtgg cggtcagcag gccggtgcgc ggcgcggcgg ccttgggcgg gtcggccggg
    11821 tcgctgccgt agggcagcag gccgacgtcg gccgggcggc cgggcaccag ccaccagacc
    11881 agcggcacca tgaccagggc ggcgatggcc acggcccaga cggcgcgcgt ccagtcgccc
    11941 gacgaggcca ggctggccag caccggcagg aagatcaggt tgccggtggc ggtgctggcg
    12001 gtgagcacgc ccatggccag gccgcgatgc ttgacgaacc agcggttgac cacggtggcg
    12061 cccagcacca cggccacggc gcccgaagcc atgcccgaga agatgcccca cagggcgatc
    12121 aggtgccagg tctgggtcat gtaggcgctg gccgcgcagg aagcggccat gactaccaag
    12181 gccgtcagca ggatgtggcg caggccgaag cgctccatgg cggcggcggc gaagggcccc
    12241 atcatgccgt agaagaagat gccgatggcc gccgccagtg aaatgatcga gcggctccag
    12301 ccgaaggtct gctgcagcgg cgtcagcagc acgctgggcg tggagcgcag cccggccgag
    12361 aacagcaggc agaagaaaat gacgccgacc accacgaagg catagcgctg gcccaggcgg
    12421 ggaaagggag aggcattgcg cgcgggagtg gggggagagc tcatgaatgt taccgatcgg
    12481 tacgggcggg attgcgtata atacgtaccg gtcagtaaca tcgtcaagcc attttcggga
    12541 ctttcagcgt cccgatccgg acatccggta ttcctgtccc gataggtggg agagcaacat
    12601 gacaaaagca cggcaagccg cggcgcggcc ggaaacccaa gggtcgacaa ccgaaccgct
    12661 gcgcgcagcc gaccgcatcc gccgtagcgc tcgcgatctg ttttaccacc agggcatccg
    12721 ggcggtgggg gtggatgcca tcgtggccga ggccggcgtg accaagccca gcctctaccg
    12781 caatttcgcc tccaaggacg aactggcggc cgcctacctg cacgattatg aaaccgtgtt
    12841 ctggagccgc ttcgacgcag ccgtggccgc gcatccgggc gacccgcgcg cgcagatcct
    12901 ggcctttttc gaaggcctgg ccgaccgggt ggcgaacatg cccgggtatc gggggtgcgg
    12961 cctgaccaat gccgccatcg aatatcccga gcccgaccat cccgcccggc tggtgtcggt
    13021 ggccaacaag cagcaggtgc gcgagcgcct gatcgacatg gcgcgccgca tgggcgcgcg
    13081 cgacccgcaa gtgctgggcg acggcctgca gctgttgatg gagggagcct atgcctccag
    13141 ccagctgctc agggttggcg ggccggtcca gcacctgccc gtcgtggcgg cacgcctgat
    13201 cgacgccagc cttcaataaa atcaggtgtg tacgctcttt tcgagtccga tccgagcggc
    13261 cgccgaaagg gctttccgca agtagtccac cgctttttcc gaagcagcgc aagaccgcgt
    13321 ttttattgct cgttctgagc atataagcgt gatttttcaa tgacatacgg gtaatcctgg
    13381 cgtgttcttc cgcgccggat ttaggtatgg tgtgtgcgct ggaatttgat aaacgacgtg
    13441 gcgcgccacg cattcaaata tcttcatggg gaaagcaatg aaatctgtct tgcggcgcct
    13501 gggcgccatg ctgagcttgt ccgtcgcggc ggttgcgcta tgcctgtccg cgccggcgcg
    13561 tgccgtcgag atttcggtca cgcaatgggg cgcgagcctg tacggcctgc cttacgccgt
    13621 ggccatggac cagggcctgt tcaagaaggc cggcgtcgac atcaccagca tcctgagctc
    13681 gggcggcggc ggcaccacgg tgcgcaacat cctggccagc gacgtgccct acggcgaagt
    13741 ggccgtctcg gctgcgctgg cggcgcagcg ccaggggctg gatctggtca tcgtcaacgt
    13801 cggcacgcgc agcgtggccg aggcttcgat ggtcaccatg cccaattccg acgtgaagga
    13861 cgtggcggac ctggtgggca agaaaatcgc catcacctcg cccaagagcg tgtccgagat
    13921 gctgttcctg atgacgctca aggaaaaggg catcgacgcc ggcaaggtcc agcgcgtggc
    13981 ctcgggcggc tacgtcaatg gcctgacgct gctggagcag ggcggcgtgt cggccgccgt
    14041 gctgatcgag cccctgtcca tcgtgcgcaa ggcgcaatac cgcacggtct accacgcggg
    14101 cgagatcctc aagccgatga ccacctcggt gggcatcacc acccgcaagt tcgccgccga
    14161 gcatcccgac gtgctcaagg ccatcatcgc cggccgccgc gccggcgtcg aggccaccta
    14221 tgccgacccg ggcgccgcgg ccaagctgct cgaatcgagc ttcaagctca cgccggaagt
    14281 cgcccgcgag gcggtcgaca acatggtcaa gtcgcgcatg tggagcgacg gcgagttcga
    14341 ccgcgccgag ctcgaccgca tggtggccgg cctgaagatc atcggcgagc tcaacgagga
    14401 cgtcgactgg agcaagctgg tcgacaccgc gttcctgccc gccgacctgc aggccaagag
    14461 caagctatga gcaatgtggc gcccttgatc aagcccgtcg ccaaacccga tgcgcccgcc
    14521 gccgcctccg cggcgcagcg cgaggcgcac gccgtgctga gcggcgtgac gcgctatttc
    14581 gacaaggcgg gtccgcgcga gcgcttccat gccctggggc cgatcgacct caccctgcac
    14641 cagggcgagt tcttctcggt ggtggggccg tcgggctgcg gcaagtcgac cttgctcgac
    14701 atcctggccg gcctggcggc gccgaccagc ggcgccgtgc tgttcgaggg gcgcgaggtc
    14761 aagggcgtgc cggacggggt cggcgtggtg ttccaggaag acgcctcgtt tccctggctg
    14821 acggtgcgcg acaacatcgg cttcggcctg cgcatggcgg gcatggacgg cgccgagatc
    14881 aagcggcgcg tcgactacgc gctgggcttc atgggcctga aggacttcgc ttcggcctac
    14941 ccggtgcaat tgtcgggcgg catgcgccag cgcgtctgca tcgcccgcac gctggtgcag
    15001 cagccgcgcc tgatcctgct cgacgagccc ttcggc
//
LOCUS       DASWPO010000113        10124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3149137, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000113 DASWPO010000000
VERSION     DASWPO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149137"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VGC69_06545"
     CDS             <1..353
                     /locus_tag="VGC69_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0294978.1"
                     /translation="GAEAAHWIDRYAREARAELAGPKVSDALFVTARAEAMSRQAFWQ
                     LIKKYAVQADVHAPLSPHVLRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTHVAR
                     ERLKALHAAHHPRG"
     gene            375..863
                     /gene="ybaK"
                     /locus_tag="VGC69_06550"
     CDS             375..863
                     /gene="ybaK"
                     /locus_tag="VGC69_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEY0294979.1"
                     /translation="MSKAKHVSETPATQLLKQHKVAYTEHPYDYVDHGGTRESARQLG
                     VDEHQVVKTLIMEDEDAKPMVVLMHGDREVSTKNLARQIDAKKVAPCKPEVAQRHSGY
                     MVGGTSPFGTRKRMPIWVEASVLALPRIYINGGRRGYLVGIEPRVLVDLLGAKPVDAA
                     LE"
     gene            983..1561
                     /locus_tag="VGC69_06555"
     CDS             983..1561
                     /locus_tag="VGC69_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0294980.1"
                     /translation="MSKKLLMLVGDYAEDYETMVPFQTLLTVGHAVHAVCPDKKAGDT
                     VATAIHDFEGAQTYSEKRGHNFALNFDFAKVDPAAYDGLVIPGGRAPEYLRLNERVLE
                     IVRHFDQAKKPIAAVCHGAQVLAAAGILRGRTCSAYPACAPEVRLAGGTYAEIAIDQA
                     CTDGNLVTAPAWPAHPAWLAQFLEVLGTRISH"
     gene            complement(1588..2502)
                     /locus_tag="VGC69_06560"
     CDS             complement(1588..2502)
                     /locus_tag="VGC69_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0294981.1"
                     /translation="MDVLKAMSIFVRVVESGSLTAAAQDCGLSPTMVGNHLQALEARL
                     GTRLMHRTTRKQQLSAFGHAYYERCIEILDLVGETDRLALNQHATPRGHLRLTAPVTF
                     GNECLIPALADYCRRYPDVKLDIAVTDSLPDLMEDGFEAAIRLGKPATPELVARPLQP
                     YRLLLCAAPSYLADHGTPGKPEDLAAHQCLTYAYPLHSELRSPRPAWMLTGPDGPISI
                     PVEGRLQVDSADGLRRAALAGMGIAMLPSVMVADDIRAGRLAEVLAGRGPAARPLNLL
                     YLRDRLMTAKLRTFVDFVVERFGQPRQP"
     gene            2622..4004
                     /locus_tag="VGC69_06565"
     CDS             2622..4004
                     /locus_tag="VGC69_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0294982.1"
                     /translation="MSLPRTPSPRLALLSASLGFVIICLDVTVVNVALERIQLALNTD
                     VAGLQWVLNAYTLAFASLLLSAGALGDRLGAKPVFIGGLALFTVASLACGLAPGIGAL
                     VAARLLQGVAAALCVPSSLALLNASFPEPRARVRAVSIWAGTASLAIGAGPIVGGLLV
                     DRFGWASVFLVNLPIGLAGIGLAWVHAPGTPRGAGHGFDLRGQALAIAALAALTFGFV
                     ESGPLGWGHPAVVAGFVGAAVAGAGFLRVERRQAHPMLPLSLFQIPVVGMSSLVGLSL
                     NFGYYGLMFALSLFFQVVKGYPPLTAGLAFLPMTAVVVIANLASGALTVRFGHKLPMV
                     AGQALAALGCFALAAIDADSRYVWTMVPLLAVGIGTALVVPSINTALLANVDRSQVGI
                     AAGVQNTARQIGGVIGVGVFGSLLGGEAGGMAQGLHVAVTLAGVGLAASCAWVVFGMR
                     ARAPCGACGP"
     gene            complement(4209..5258)
                     /gene="pbpG"
                     /locus_tag="VGC69_06570"
     CDS             complement(4209..5258)
                     /gene="pbpG"
                     /locus_tag="VGC69_06570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEY0294983.1"
                     /translation="MAFQWVRAVTLTTATVSLAAGVLFSSPVLAATTAVCKSHPATTA
                     CKSAAKKTDSNTAAKARKGHAVGKSAAKGKKGKKVARRAISPEALLAAVSTSPGGNDA
                     AALRSTTAFVQDMHTSTVLFAKNENVVRPIASISKLMTALVVLDAHQPMDDMIEVTDD
                     DIDMLKHTSSRLPVGARLSRNDMLHLALMASENRAANSLGRNYPGGLPAFVAAMNAKA
                     RSLGMINTHFIEPTGLSSENVSSPQDLALLLRAASQQPLIHQYTTASDYTVQVKSRMV
                     TFRNTDMLVRRPDWDIRVSKTGFINEAGQCLVMMARIEGHDMAIVLLDSHGTLSRIGD
                     AVRIRRILERQIAML"
     gene            5827..5902
                     /locus_tag="VGC69_06575"
     tRNA            5827..5902
                     /locus_tag="VGC69_06575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5860..5862,aa:Arg,seq:cct)
     gene            5915..6766
                     /gene="serB"
                     /locus_tag="VGC69_06580"
     CDS             5915..6766
                     /gene="serB"
                     /locus_tag="VGC69_06580"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY0294984.1"
                     /translation="MLLVLQSPEPWSPEAIAESAALCGATRTVPVDARRIRHETQLPP
                     ASWRESLLAFCARHQADAAMLHAPRPLPGYRLMVFDMDSTLIDIECIDELAARVGRGA
                     EVAALTDKAMRDPTVAYDDSLRARVRCLQGMTLAEMEAVYAERVRFNPGARELIADAR
                     GLGLRTALVSGGFNEFARRVQRELGIDEVVCNGLETRDGRLTGEMTGTLVNAREKARA
                     LARFCDQIGCPTTDAIAVGDGANDLEMMAIAGLSVSYRAKPVVRRRADVLIDHGTLHD
                     LVALLAG"
     gene            6879..7706
                     /locus_tag="VGC69_06585"
     CDS             6879..7706
                     /locus_tag="VGC69_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0294985.1"
                     /translation="MSRQDPIPNIQRNLRALVGQFESVAEFCRKIGVNRQQFNKYLAG
                     VHAPSNKTMRHLADAFGLSVDDFLLGRQEFLAKMATGRPDSPAPRADNTHFEKFQALA
                     VGSAAQLKPFLGTYFRYHHSSIYPGHIVRAVTIIYSAGPVVKYLTVERFPMPAGEGKG
                     SYSFFYSGICYLLGDRLFMLDYERRQENELTLAVLMPQYRTPMKYLFGLLTGVASTTF
                     RQPFSSRVVLEKRSDDTTVRKGKLRQATVAKPGDPGISPTVARYFAADDRKLLWGQE"
     gene            complement(7728..8507)
                     /locus_tag="VGC69_06590"
     CDS             complement(7728..8507)
                     /locus_tag="VGC69_06590"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY0294986.1"
                     /translation="MSTRDPLNNLATPTRNALRNTRLDLAAALRCAAKLGFHEGTCNH
                     FSATVPGLPNCYLINPFGLHFAEIRAGDLLLLDADGQVIEGEGTVEASAFYIHSRVHQ
                     ANPGAACVLHTHMPYATAITSLRDGKLALCHQNAARFYGRIAYDDDAGGFQGLALATD
                     EGDRIARALGDKSVLMLRSHGPVVTGPTIAQAFDDLYYLERVAQVQLLAWSSGQPLAD
                     IARDKAAEVARDFAEQAPVYAHQHFEALKRQLDRESPDWRG"
     gene            complement(8519..9847)
                     /locus_tag="VGC69_06595"
     CDS             complement(8519..9847)
                     /locus_tag="VGC69_06595"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HEY0294987.1"
                     /translation="MTTPPDLPMLPAALHAPLQAGRPLLWLNPGLGGALPARAPSAAD
                     VGDAARRLARSAGLLQALFPETCATSGIIDSPLLDVPALQAGLGLDRTQLGTLLLKAD
                     HLLPVAGSIKARGGFHEVLAHAEALAVEAGLMDHEGDLAALRAPAAHELFSQRTIAVG
                     STGNLGMSIGIMAAALGFSAVVHMSADAKPWKKDRLRERGVMVVEHAGDYAAAVAAGR
                     AQCAVDPNGYFVDDENSLLLFLGYAAAARQLGDQLAQAGRTPSSDAPLFVYLPCGVGG
                     APGGIAYGLKQLFGAHVHCFFAEPTASPCMLVQLAAGVGRNVSVYDVGLDNRTDADGL
                     AVPKASPLASSLMAPLLSGIHTVDDAQLYLDLYQAWRTQGLEIEPSAAAGFGGPRWLA
                     QTPEGRGYLDLHGLVRSLARATHVVWSTGGGLLPEKEHLRFRATAESLAP"
     gene            complement(9844..>10124)
                     /locus_tag="VGC69_06600"
     CDS             complement(9844..>10124)
                     /locus_tag="VGC69_06600"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0294988.1"
                     /translation="GALFLGARTTVSYGDKVIGTNHTLPTMGAARYTGGLWVGKFIKT
                     CTYQRVLTDEASALIGEYCSRLCAMEGFAGHKEQADLRVRRYGAAAAV"
BASE COUNT         1645 a         3105 c         3504 g         1870 t
ORIGIN      
        1 tgggcgcgga agccgcgcac tggatcgacc gctatgcgcg cgaggcgcgc gccgagctgg
       61 ccggcccgaa ggtgagcgat gcgctcttcg tcacggcccg cgccgaagcc atgtcgcgcc
      121 aggccttctg gcagttgatc aagaaatacg ccgtgcaggc cgacgtccac gcgcccttgt
      181 cgccgcacgt gctgcgccat gccttcgcca cgcacctgct caaccacggc gccgacctgc
      241 gcgtggtgca gatgctgctg gggcacgccg acatctccac cacgcagatc tacacccacg
      301 tggcgcgcga acggctcaag gccctgcatg cggcgcatca cccgcgcggg tgaggatcta
      361 aacttgcccg catcatgagc aaagccaagc acgtttccga aacacccgcc acccagctgc
      421 tcaaacagca caaggtggcc tacaccgaac acccctacga ctacgtcgac catggcggca
      481 cgcgcgagtc ggcgcgccag ctgggcgtgg acgagcacca ggtggtcaag accctcatca
      541 tggaggacga ggatgccaag cccatggtgg tgctgatgca cggcgaccgc gaggtgtcca
      601 ccaagaacct ggcgcgccag atcgacgcca agaaggtcgc gccctgcaag cccgaggtgg
      661 cgcagcgcca ttccggctat atggtgggcg gcacctcgcc cttcggcacc aggaagcgca
      721 tgccgatctg ggtcgaggcc tcggtgctgg ccttgccgcg catctatatc aacggcggac
      781 ggcgcggcta cctggtgggc atcgagcccc gggtgctggt cgacctgctg ggcgccaagc
      841 cggtggacgc ggcgctggaa taggcgggtt tcgactggtc acgacaacgt catatcggcg
      901 cggtgcgctt gccgcataat gagcacgaca ataaaaaagt cgggcgatgg aatgatcccc
      961 gcccgcatcc acggagacgc tcatgagcaa gaaactgctg atgctggtcg gcgactatgc
     1021 cgaagactac gaaaccatgg tgcccttcca gacgctgctg acggtggggc atgcggtgca
     1081 cgcggtgtgc cccgacaaga aagccggcga caccgtggcc acggccatcc acgatttcga
     1141 gggcgcgcaa acctacagcg aaaagcgcgg ccacaacttc gccttgaatt tcgacttcgc
     1201 caaggtcgat ccggccgcct acgacggcct ggtgattccc ggcggccggg cgcccgagta
     1261 cctgcgcttg aacgagcgcg tgctggagat cgtgcggcat ttcgaccagg ccaaaaagcc
     1321 gatcgccgcc gtatgccatg gcgcgcaggt gctggccgcg gcgggcatac tgcggggccg
     1381 gacctgctcg gcctatccgg cctgcgcgcc cgaggtgcgg ctggccggcg gcacctatgc
     1441 cgagatcgcc atcgaccagg cctgcaccga cggcaacctg gtgaccgcgc cggcctggcc
     1501 cgcgcatccg gcgtggctgg cgcagtttct cgaggtgctg ggcacgcgga tttcgcactg
     1561 agggcgtctc tccctccggc gacgattcta gggttgccgg ggctggccga agcgctcgac
     1621 gacaaaatcg acgaacgtgc gcagcttggc ggtcatcagc cggtcgcgca gatagagcag
     1681 gttcagcggc cgggcggccg gcccacggcc ggcgagcact tcggccaggc ggccggcccg
     1741 aatatcgtcg gccaccatga cggagggaag catggctatg cccatgccgg ccagcgcagc
     1801 ccggcgcagg ccgtccgcgc tgtcgacctg caggcgtccc tcgaccggga tgctgatagg
     1861 gccgtccggc cccgtcagca tccacgccgg tctgggcgag cgcagttcgg agtggagcgg
     1921 gtaggcgtag gtcaggcact ggtgcgcggc caggtcttcc ggcttgccgg gcgttccatg
     1981 atcggcgaga taggacggtg cggcgcacag cagcaggcga tacggctgga gcgggcgcgc
     2041 caccagttcc ggcgtcgcag gcttgcccag ccggatagcc gcctcgaacc cgtcttccat
     2101 caggtcgggc aggctgtccg tcaccgcaat gtccagcttc acgtccgggt agcgccggca
     2161 atagtccgcc aaggcgggaa tcaggcattc attgccgaag gtcaccgggg cggtgaggcg
     2221 caggtgcccg cgcggcgtgg cgtgctggtt gagcgccagg cggtcggttt ccccgaccag
     2281 gtccagtatc tcgatgcagc gctcgtagta ggcgtgcccg aacgcgctca actgctgctt
     2341 gcgtgtggtg cggtgcatga gccgggtgcc taggcgggcc tccagcgcct gcaggtggtt
     2401 gccgaccatg gtcggcgaca ggccgcagtc ttgcgcggcg gccgtcaggc tgccggactc
     2461 gaccacgcgg acgaagatgg acatggcttt caatacatcc attacctaat tttatttggt
     2521 tttaaatgaa ttaaaaccag gtttatcaat taataattag taattaaagt gccggttcta
     2581 tcaaccctcg cggctttcca cgctgcgccg gaatcgacgc catgtctctc ccccggacgc
     2641 cttcccctcg tcttgccctg ctgtcggcca gcctgggttt tgtcattatt tgcctcgacg
     2701 tcacggtggt gaacgtcgcg ctggaacgca tccagcttgc cttgaacacg gacgtcgccg
     2761 ggctgcagtg ggtgctcaat gcgtacacgc tggcttttgc cagtctcttg ctcagcgcgg
     2821 gcgcgctggg agacaggctg ggcgccaagc ccgtattcat cggcgggctg gcgctgttca
     2881 cggtggcgtc gctggcttgc ggcctggcgc ccggtatcgg cgcgctggtt gccgcgcggc
     2941 tgctgcaggg ggtggcggcg gcgctgtgcg tgccgtcatc gctggcgctg ctcaacgcca
     3001 gttttcccga gccgcgggcg cgcgtcaggg cagtcagcat ctgggccggc acggcgagcc
     3061 tggcgatcgg cgcgggcccc atcgtgggcg gcctgctggt ggatcgtttc ggctgggcga
     3121 gtgtttttct cgtcaacctg cccatcggcc tggccggcat tgggctggca tgggtccatg
     3181 cgcccggcac gccgcgcggc gccgggcatg ggttcgacct gcgaggccag gccctggcca
     3241 tcgccgcgct cgccgcgttg acattcggct tcgtcgagag cggtccgctg ggttgggggc
     3301 atccggccgt tgtggcgggc tttgtcggcg cggccgtcgc gggagccggg ttcctgcgcg
     3361 tggagcggcg ccaggcgcat ccgatgctgc cgctgtcctt gttccagatt cctgtcgtcg
     3421 gcatgagttc cttggtcggc ttgtcgctca atttcggcta ttacgggttg atgttcgcgc
     3481 tcagcctgtt tttccaggtc gtcaaaggct atccacccct gaccgcgggc ctggcgttcc
     3541 tgcccatgac ggcggtcgtg gtgattgcca acctggcgtc cggtgcgctg acggtgcgct
     3601 ttggccacaa gctccccatg gtggccgggc aggccctggc tgcgctgggt tgcttcgcgc
     3661 tggccgccat cgacgcggac agccgctatg tgtggaccat ggtgccgctg ttggctgtcg
     3721 gcatcggcac ggccctggtc gtgccatcga tcaatacggc cttgctcgca aacgtggacc
     3781 gctcgcaagt cggcatcgcc gcgggagtgc agaacaccgc gcgccagatc ggcggcgtca
     3841 tcggcgtcgg tgttttcggt tcactgcttg gcggcgaggc gggcggcatg gcgcagggcc
     3901 tgcatgtggc ggtcacgctg gcgggggtgg gcctggcggc cagctgcgca tgggtggttt
     3961 tcggtatgcg ggccagggcg ccatgcggcg cgtgcgggcc gtgacgtggc gcgtggccgt
     4021 gccggaccga gggttcggcg ggcgagtgta ttgacggccc gttcgttgac ggcacgttta
     4081 gatgcttttc atcgattctt atgtgtgccg catcatgcaa aacaccccga aagcctggac
     4141 tgtcgtccgg ctttcggggt gcgtttcgaa ccgctgtctt gtgcgccgcg cctggggcgc
     4201 ggcgtgatct acagcatggc gatctggcgc tcgaggatgc ggcggatgcg cactgcgtcg
     4261 ccgatgcgcg agagcgtgcc gtgcgagtcg agcagcacga tggccatgtc gtggccctcg
     4321 atgcgcgcca tcatcaccag gcactggccg gcctcgttga tgaagccggt cttggagacc
     4381 ctgatgtccc agtcgggacg gcgcaccagc atgtccgtgt tgcggaaggt caccatgcgc
     4441 gacttcacct gcaccgtgta gtccgaggcg gtggtgtact ggtggatgag cggctgctgg
     4501 gaggcggcgc gcaacagcag ggccaggtcc tgcggcgaag agacgttctc gctggacagg
     4561 cccgtgggct cgatgaagtg ggtgttgatc atgcccaggg aacgggcctt ggcgttcatg
     4621 gcggccacga aggccggcag gccgccggga tagttgcggc ccagcgagtt ggcggcgcgg
     4681 ttctcggaag ccatcagcgc caggtgcagc atgtcgttgc gcgacaggcg ggctcccacc
     4741 ggcaggcgcg agctcgtgtg cttgagcatg tcgatgtcgt cgtcggtgac ttcgatcatg
     4801 tcatccatgg gctggtgcgc gtcgagcacg accagggccg tcatgagctt ggagatcgaa
     4861 gcgatgggac gcaccacgtt ttcgttcttg gcgaacagca ccgtggaggt gtgcatatcc
     4921 tgcacaaagg ccgtggtgga gcgcagcgcg gcggcgtcgt tgccgccggg cgaggtggac
     4981 acggcggcca gcagggcttc ggggcttatg gcgcggcggg cgaccttctt gcctttcttg
     5041 cccttggcgg cgcttttgcc cacggcgtgg cctttgcggg ccttggcagc ggtgttggaa
     5101 tcagttttct ttgcggcaga tttgcaggcc gtcgtggcgg gatggctctt gcagacggca
     5161 gtcgtggcgg ccaggacggg ggaggagaaa agcactcccg ccgcgaggga cacggtagcc
     5221 gtggtcaacg ttactgcgcg cacccattga aatgccatga tgtaagcaga ccttgagtgt
     5281 gtatatagat tttgcgttgt tcaccggatc tggggctttg gttaaaactc cagataaatt
     5341 agcgagttac ggacggaatt taaacaggga tttaaggttg ttggcaagca atagcatgaa
     5401 tgatggcata tttttaataa gggacgcttt gtgggcgttt gcatcttttc actggatttt
     5461 tcattttgca ggcataaacc ttgtcatgga cagggttagc cctccaaagt ttcccaacct
     5521 ggcacgctgt ccatatcgat gtgtttcaac ccactccgaa aaggaagccg cctcatctcc
     5581 agcgtcgctg gcgttacccc catggcagca agcgggtcct tatccctgct gaccgcgatc
     5641 cgggtcattt cgggcttttt ctacttgccg cacgcgtact cgcggccgat gccgtgcatg
     5701 ctgcgtcggg cgccgtgcgc gggtagtgga ccttcggcgg catcggcacg ggcatcaggc
     5761 tgtaggatta ggatcgacgc ctgcgcgtcg cgcgcatgcg ggcgagccgg ctacaatgcc
     5821 gcgctcgccc ccgtagctca gtggatagag cgatcccctc ctaagggata ggtcgcaggt
     5881 ccgattcctg ccgggggcac caaacctttt cctcatgctg ctcgtcttgc aatcgcccga
     5941 gccgtggtcc cctgaggcca tcgcggaatc cgccgccctt tgcggcgcca cgcggacggt
     6001 acctgtggat gcgcgccgga tccgccacga aacccagttg ccgcccgcat cctggcgcga
     6061 atcgctgctg gctttctgcg cgcgccatca agccgatgca gccatgctgc acgcgccccg
     6121 tcctttgcct ggctatcgct tgatggtatt tgacatggac tccacgctca tcgatatcga
     6181 gtgcatcgat gaattggcgg cgagggtggg gcgaggggcg gaggtggctg cgttgacgga
     6241 caaggccatg cgggatccga ccgtggcgta tgacgacagt ctgcgggcgc gggtgcgctg
     6301 cctgcaaggc atgacgctgg cagagatgga ggcggtgtac gcggaacggg tgcgattcaa
     6361 cccgggggct cgggaactga tcgcggacgc gcgcgggcta ggattgcgta cggccctggt
     6421 gtcgggcggg ttcaatgaat ttgcccggcg ggtgcagcgg gaactaggca tcgacgaagt
     6481 cgtgtgcaac gggctcgaaa cgcgtgacgg ccggctgacg ggagaaatga cgggcacact
     6541 ggtcaatgcg cgcgagaaag cgcgtgcgct ggcgagattc tgtgatcaga taggttgtcc
     6601 aaccacagac gcgatcgcgg tgggcgatgg ggcaaatgac ctggaaatga tggcgatcgc
     6661 aggcttgtcg gtaagctatc gggccaagcc ggtagtgcgt cggcgcgccg acgtgctgat
     6721 cgaccacggt acgctgcacg acctggtggc cttgctggca ggctaagcag gtttttagtg
     6781 gattggctgt attaaattag acgacctcgg acggtacccc taaaggcgaa cctgtctgaa
     6841 ctatgcatgt ccatgccttt ttccaagggg ttggcttagt gagccgtcaa gatccgattc
     6901 cgaatatcca gcgcaatttg cgagcgttgg tggggcagtt cgagtccgtg gcggaattct
     6961 gtcgcaagat cggtgtcaat cgccagcaat tcaacaaata cttggccggt gtgcatgcgc
     7021 cctcgaacaa gacgatgcgg catctggcgg atgctttcgg cttatcggtg gatgatttcc
     7081 tcctggggcg ccaagagttc ctggcgaaga tggccacggg acggcccgat agtccggccc
     7141 ctagggcaga caacacgcac ttcgaaaaat tccaggcgtt ggcggtcggc tcggcggcgc
     7201 aactcaagcc gtttctgggc acctacttcc gctaccacca ttcgtccatc tatcccggcc
     7261 atattgttcg tgccgtgacg atcatctact cggctggccc ggtcgtgaaa tatttgacgg
     7321 tcgaacggtt tcccatgcca gcgggcgagg gcaagggcag ttattcattc ttttattccg
     7381 gcatctgcta cctgctgggc gatcgcctgt ttatgctcga ttacgagcgg cggcaggaga
     7441 atgagctgac gctggcggtg ttgatgcctc agtaccgtac gccgatgaag tatttgttcg
     7501 gattgctgac gggggtggct tcgacgacgt ttcgccagcc gttctccagc cgcgtggtac
     7561 tcgagaagag atccgacgac acgactgtgc gcaagggcaa gctgcgccaa gccaccgtgg
     7621 cgaagccggg cgatccgggg atttccccga ccgtcgcccg ctatttcgcc gcggatgacc
     7681 gcaagctgct gtggggccag gaatagcagc gcaggcgcgc gcggatttca accgcgccag
     7741 tcggggcttt cgcggtcaag ctgccgtttg agcgcttcga agtgttggtg ggcgtagacg
     7801 ggtgcctgtt ccgcgaagtc gcgagcgact tcggcggcct tgtcccgcgc gatgtcggcc
     7861 agcggctggc cggacgacca ggccaggagc tgtacctggg ccacgcgttc caggtagtag
     7921 agatcgtcga aggcctgcgc gatggttggg ccggtgacga cggggccgtg actgcgcagc
     7981 atcagcaccg atttgtcgcc cagtgcccgc gcgatacggt cgccttcatc cgtcgccaat
     8041 gccaggccct ggaagccgcc cgcgtcgtcg tcgtaagcga tgcggccgta aaagcgggcc
     8101 gcgttctggt ggcatagcgc cagtttgccg tcgcgcagcg acgtgatcgc ggtggcgtag
     8161 ggcatatggg tatgcagcac gcaggccgcg cccggattgg cctggtgcac gcgcgaatgg
     8221 atgtagaagg ccgaggcttc caccgtgcct tcgccctcga tgacctggcc gtcggcgtcg
     8281 agcagcagca ggtcgccggc gcggatttcc gcgaaatgca aaccgaaggg attgatcaga
     8341 taacagttgg gcagtccggg aacggtggcg ctgaaatgat tgcaggtgcc ctcgtgaaaa
     8401 cccagtttgg cggcgcagcg caacgcagcg gccagatcca ggcgtgtatt gcgcagggcg
     8461 ttgcgcgtag gcgtcgcaag attgttgaga gggtcgcgtg tggacatggt cgtttacctc
     8521 aaggcgccag gctttcagcc gtcgcgcgga atcgaagatg ttctttttcg ggcaacagtc
     8581 cgccaccggt agaccacact acgtgcgtgg cgcgggcaag ggagcggacc agtccgtgca
     8641 ggtccaggta gccgcggcct tccggcgtct gggcgagcca gcgcggtccg ccgaagccgg
     8701 cagcggccga gggctcgatt tccaggccct gcgtgcgcca ggcctggtaa aggtcgaggt
     8761 agagctgcgc atcgtccacg gtatgaatgc cggaaagcag cggcgccatc agggagcttg
     8821 ccagcggcga ggccttggga acggccaggc catcggcgtc ggtccggttg tccaatccga
     8881 cgtcgtacac cgatacgttc cggccgactc cggcggcgag ctgcacgagc atgcaaggag
     8941 aggccgtcgg ttcggcgaag aagcaatgga cgtgcgcgcc gaacaactgt ttcagcccat
     9001 aggcgatgcc gcccggcgcg ccgccgacgc cgcacggcag gtagacaaac agcggcgcat
     9061 cgctggacgg cgtccggccg gcctgtgcca actggtcgcc cagttgacgg gccgccgctg
     9121 catagccgag gaacagcagc agggagttct cgtcgtcgac gaagtagccg ttcgggtcga
     9181 cggcgcattg ggcgcgtccc gcggccaccg cggcggcgta gtcgccggcg tgttcgacaa
     9241 ccattacgcc gcgctcgcgc agccggtctt ttttccaggg cttggcgtcg gccgacatgt
     9301 ggacgacggc cgagaagccc agcgcggccg ccatgatgcc gatgctcatg cccagattgc
     9361 ccgtgctgcc aaccgcaatg gtgcgttgcg agaagagttc gtgcgcggct ggcgcgcgca
     9421 gcgcggcaag atcgccttcg tgatccatca gtcccgcctc gacggccaat gcctcggcat
     9481 gcgcgagcac ctcgtgaaag cctccgcgcg ccttgatgga gcctgccact ggcagcaagt
     9541 ggtcggcctt cagcagcagc gtgccaagtt gcgtgcgatc cagccctagc ccggcttgca
     9601 atgcgggcac atccagcagc ggggagtcga tgatgccgct ggttgcgcaa gtctccggga
     9661 atagcgcctg cagcagcccg gcgctgcgcg ccaggcgccg tgctgcgtcg ccgacgtcgg
     9721 ccgccgacgg cgcccgggct ggcagcgcgc cgcccaggcc ggggttgagc cagagcagcg
     9781 gccggcccgc ttgcagcggc gcgtgcagcg ccgcgggcag catgggcaag tccggcggcg
     9841 tggtcatacg gcggccgcgg cgccgtagcg gcgcacgcgc aggtcggcct gttccttatg
     9901 gccggcgaag ccctccatgg cgcacagcct cgagcagtat tcgccgatca gggcggaggc
     9961 ttcatcggtc agcacgcgct ggtaagtgca agtcttgatg aatttgccga cccacagccc
    10021 gccggtatag cgcgccgcac ccatggtggg cagcgtgtgg ttggtgccga tgaccttgtc
    10081 gccataggaa acggtggtgc gcgcgcccag gaacagcgcg ccgt
//
LOCUS       DASWPO010000114        16013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2881496, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000114 DASWPO010000000
VERSION     DASWPO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 16013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16013
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2881496"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGC69_06605"
     CDS             complement(<1..533)
                     /locus_tag="VGC69_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0294989.1"
                     /translation="MNLQTALAHHRAGRLAQAVAIYQQILEANPNDSEALHWLGNAAG
                     QLGEIDLAVTLLAKAHTLAPRNAQYLLSLGAMHRARQAYEPSLACYEQALALQPGSGA
                     ACYGKGMALQRLGRTQDAMAALRKALALAPNHAQATLSLAYLLRETGELDEAARHYRQ
                     AIATTPALASAHYGLGIA"
     gene            complement(669..1427)
                     /locus_tag="VGC69_06610"
     CDS             complement(669..1427)
                     /locus_tag="VGC69_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0294990.1"
                     /translation="MLVKQAANVLDLLEYFATRQQPATLAEISLHFGWPRSSTFNLLG
                     TLSERGFLYEPASRGSFYPTPRWRALAEAVAGAEPLPQVVQDLLEEVWRATNETTVIL
                     APMGPHAVFLETIESRQAVRYSAYSGKLVPIHTTASGQALLSLLPEAERKILLRKISY
                     PAYTKTSLTDAQEVEAAVKAGIKRGWFESVGGFTADLGGIAVPISYPDRRLAILVGGP
                     IDRVRPRYAALAKIIQQAMQKLGLPRAGQPARKP"
     gene            1607..2773
                     /locus_tag="VGC69_06615"
     CDS             1607..2773
                     /locus_tag="VGC69_06615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0294991.1"
                     /translation="MDFALTQEQEAIRDSIAKVCEGFGDDYWLERDSDGQFPHAFYDA
                     LAQAGWLGVCIPEAYGGAGLGISQAAIMARTIAESGAGMSGASAVHINIFGLNPVAVF
                     GTEEQKQRMLPPMAQGRDKTCFAVTEPNTGLNTTQLKTRAVRKGDRYVVNGQKVWIST
                     AQVANKMLLLARTTPLEAVKKPTMGLSLFYTDFDRSRVAVHLIEKMGRKAVDSNELFF
                     EDFDIPVEDRIGEEGRGFEYILHGMNPERILIGSEAVGLGSLALTRATAYAKERIVFN
                     RPIGQNQAIQHPLAANWAELEAAWLMLLKAAWQYDSGQSCAALANAGKYLAGEAGFNA
                     CQQAVMTHGGFGYAKEYHVERYLREVMIPRIAPISPQLVLSFIAEKVLGLPKSY"
     gene            2805..4076
                     /locus_tag="VGC69_06620"
     CDS             2805..4076
                     /locus_tag="VGC69_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0294992.1"
                     /translation="MGPRIIEAADLRLGEFIRPGDTVVCGQTAAEPLTLTQALVEQRA
                     DLSGARVFLGAVFSRTFAPEHADHLRFSSYGVLGAAAALGRQGLIDVLPCSYHELECR
                     YASGALAADVVLLQLAPGGGDGPYSLGMANDYVAAAARRARVVIAEVHPDVPWTHGAA
                     WPRDARIDVVVPATRPPLDMAAAACGPVEEAIGRHVAALIPDGAVLQTGIGAIPDAVL
                     AALRSHRDLGLHSGMAGDRMVELIEAGVVTNARKPFDAGIGVVNVLAGTASTRRHAHR
                     NPALIVRPARYTHAQAVLSTLHALHAINSALQVDLGGQVNSEMLGGVPMGGIGGLNDF
                     VRGARAAPGGRSIIALPSTARSGAVSRIVARLDDAVVTVPRCDADLVVTEWGVADLRA
                     CTLAERARRLTAVAAPQFRESLEKAWRGARQ"
     gene            4111..4569
                     /locus_tag="VGC69_06625"
     CDS             4111..4569
                     /locus_tag="VGC69_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0294993.1"
                     /translation="MAGLYYEEFSVGQQFDHPWTRTVTEMDNTLFSLLTMNPQPLHLD
                     AHFSAKTEWGQRLFNSLYTLGIMIGMAVSDTTLGTTIGNLGMTEVKFPKPVFHGDTLR
                     AQTRVIAMRESKSRPNAGLVEFEHTAFNQDGTVVATCLRTALMYRKGARP"
     gene            4566..5414
                     /locus_tag="VGC69_06630"
     CDS             4566..5414
                     /locus_tag="VGC69_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY0294994.1"
                     /translation="MKSFLFVPGDSGRKFEKASQGAADALILDLEDSVAADAKPQARQ
                     ATAAMLRQPRGSQRRYVRVNALDTGMTLADLAAVMPAAPDGIVLPKCRGGDDVRLLAH
                     YLDAFEAAHGLAGGGTRIIAIATETAESVFGLGSYRGCSERLWGLMWGAEDLSASLAS
                     SANRVDGAYTGPYVMARNLCLMGAAAAEVAAIDTVYVDIEDVAGLGEEARQARRDGFA
                     AKAVIHPRHVDAVNQAFTPTPEETAWARTIVAAFAAAPGAGVFKLNGQMIDKPHLRRA
                     QAILGQ"
     gene            5417..6613
                     /locus_tag="VGC69_06635"
     CDS             5417..6613
                     /locus_tag="VGC69_06635"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0294995.1"
                     /translation="MGPLAGFKILDMTAVLMGPSATQVLGDMGAEVIKVESPDGDMVR
                     QIGPSRHPGMGPIFMHVNRSKRSIVIDLKQAQGRALLLRMAARADVLVYNMRPQAMAR
                     LGLTYEALAGVNPGLVYAGLFGFGQDGPYADRPAYDDLIQGAATIPYLFVRANGGEPR
                     YAPSAIADRIVGLSAVGAILAALLERGRTGLGQRVDVPMLETMVGFVLADHLGGLTFE
                     PPLDRGGYARQLSPNRKPYRTRDGYVCAMVYTDKQWRSFTQAIGVPDLMDQDPRFADF
                     KARTANIDFVYTELTRWFLERTSREWLALLERADIPSMPMHDLESVLDDLHLKAVGFF
                     PVVAHPTEGAVRTLRVAASWSRTPARPERLPPVLGQHTVEVLRELGLDDAEIGALRES
                     GAVVQA"
     gene            6691..7650
                     /locus_tag="VGC69_06640"
     CDS             6691..7650
                     /locus_tag="VGC69_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0294996.1"
                     /translation="MPQQPRLDYVTCTSPAGLHRMAYWEWGDPANDQVLVCVHGLTRT
                     GRDFDELARALAGDFRVVCPDVVGRGASDWLLNPSFYTVPQYVSDMVTLIARLRPASL
                     NWVGTSMGGLIGLGVAGTAAMTAMMRAADIRPGAQSEDLPDGASAPGRIRFDRLVLND
                     VGPRLELSALGRIGQYVGQPAWHATFAEAVRAVRETAATFGPHTDEQWETLTRHVYRQ
                     RDGRWVKHYDLGLAVPLVGQTPDAYATGEQLLWQAYDALDCPVLIVRGAESDLLSAAT
                     VAEMRARNARATSVEFAGVGHAPTLMTAAQIAPVAAFLRSSLQ"
     gene            7655..8500
                     /locus_tag="VGC69_06645"
     CDS             7655..8500
                     /locus_tag="VGC69_06645"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HEY0294997.1"
                     /translation="MDTALLNIDLHCHSSVSDGQLPPAAVARRAQGNGVDVWALTDHD
                     EVDGLAEAGRTARELGMRFITGVEVSVTWANQTVHIVGLNFDPADQTLIEGLRATRSG
                     RGERARRMGERLAALGMPGAFEGALTFAGNPELISRTHFARYLASAGYCPDVQTVFNK
                     YLHDGGPGHEPVQWATLAQAVSWICGAGGRAVVAHPGRYKYTPVQFAALFDEFIQLGG
                     EGVEVVTGSHSPEDARKYAGEARRYGLLASRGSDFHSVQDSRIDLGMLPMLPPDLKPV
                     WHDWF"
     gene            8502..9065
                     /gene="greB"
                     /locus_tag="VGC69_06650"
     CDS             8502..9065
                     /gene="greB"
                     /locus_tag="VGC69_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY0294998.1"
                     /translation="MNKAFVKESEHDDEDDIPEAQALPAGTRNYMTPQGYAQLRDELA
                     QLMNVERPAVVQVVSWAASNGDRSENGDYLYGKKRLREIDRRMRFLTKRLDIAEVVDP
                     GLQPNRDQVFFGATVVYLDRAGVERTVTIVGVDEAEPLAGRISWISPVARALIKAREG
                     DTITLRTPGGVDELEILAVHYPEPLPK"
     gene            9126..9521
                     /locus_tag="VGC69_06655"
     CDS             9126..9521
                     /locus_tag="VGC69_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HEY0294999.1"
                     /translation="MRSHRARFRTAWLGLLAMFLVVFAPLVSQSLAATRAAQAQAIEL
                     ELCSVDAHAAPAHPDHAGMHGDLLAACGYCDFLAGHAALPAVPPAALALIVLVLIAMV
                     TMPSLRHIAFGAFPSGRPRAPPILSRLAV"
     gene            9600..11978
                     /locus_tag="VGC69_06660"
     CDS             9600..11978
                     /locus_tag="VGC69_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0295000.1"
                     /translation="MRTVFSHGAHASMHGLHGRGRAMLAMAAWCLMQGQARAAGNNPQ
                     ELQTVQVTGSAPSYAEQNLLPQTTESVTAPQIAETVNAMGVEDALKYLPDVLVRKRYI
                     GDTQAPMATRTTGINASARSLLYADGILLSSLINNNNQNGSPQWFMVSPSEIERIDVM
                     YGPYAAEFPGNSYGAVTRIATRMPDRFTGSVDVIGSVQAFGQYGTHVSEGAQQYSATL
                     GDRSGQFSWRLSARHLDSDSQPITYGTLTQSQTPAGAGLPFVSGAWAGTNRAGSPMQI
                     IGAGNFTHTSQDNATLKLAYDFTPDLTATYTLGYWQNHARTYARSYLSSADGTPYYGA
                     ASGNVNLGGYAYGASAIAGQFSDGQVEQEHLMQSLEVGTHSDGLFDWQAALSNMRYLQ
                     DLSTTSTGVFPQAQNGGAGRITDMGGTGWTTLDLKGTWRPQGESGAHIVSAGLHDDRF
                     TLDSPTYTTTDWLGGGEGSLYSNALGKTETRALWLQDTWRLAPDWTATLGGRYEWWQA
                     FDGYNYAIGSNGAGVPIAQPGVRQSGLSPKASLAWQLDDAWSLTGSFGKALRFPTVGE
                     LYQSVQTGSTYLQANPYLKPERVLSSELALARNTDDSHWRLSLFNEDAADALISQTAF
                     LPGFATPVSYTQNVGRTRQTGAELAWRQDHVLWHGLSLSGNLTYVDARILSNDGFASA
                     TGTTSVGKRVPYVPTWRATLAATYRPDDKWSYTLAGRFSSRVYASVDNSDVNAAAFTG
                     FQSFLVFDARVHYQMDKHWSMALGVDNLGNSKYFLYHPFEQRTYYAEVKYAF"
     gene            complement(11975..12835)
                     /gene="rlmJ"
                     /locus_tag="VGC69_06665"
     CDS             complement(11975..12835)
                     /gene="rlmJ"
                     /locus_tag="VGC69_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY0295001.1"
                     /translation="MFSYRHAFHAGNHADVLKHAILLHTLDYLNRKDAPYWVIDTHAG
                     AGLYQLDSDWSSKNAEYAGGIAQLWDKADLPELLADYVAHIRQYNPDGQLRYYPGSPW
                     LTLDALRDKDRLRLFELHPSESEVLVRNLEKRGRGPLRQTTVFDADGFEGMKSLLPPP
                     TRRGLVLIDPSYEDKRDYRRAFNAVKEGIKRFATGTYAVWYPLVQRVEAGELARQLPH
                     LQVKSWLHATLTVQKPAADGFGLHGSGMFLVNPPWTLQAALKTALPYLARELALDDRA
                     GYTLDYRENP"
     gene            complement(12835..13584)
                     /gene="ugpQ"
                     /locus_tag="VGC69_06670"
     CDS             complement(12835..13584)
                     /gene="ugpQ"
                     /locus_tag="VGC69_06670"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY0295002.1"
                     /translation="MTTPLAPWPYPAHIAHRGGGRLAPENTLAAMRAGKRRGYRMVEF
                     DVKLSRDNVAVLLHDDVVDRTTDGHGPAREKTYAELAQLDAGGWHSPAFAGEPVPTFA
                     AVARYAVANGIACNVEIKPCPGRDAETGEAVARLAQQFWAGVHKRPLLSSFSEEALAA
                     AQAAAPELPRALLVETVPDDWPARLRQYACVGLNINQKDATPELAAAVHAAGYRLAAW
                     TVNDPDRARLLFSWGVDAIFTDQLTLIQPTA"
     gene            13701..15662
                     /locus_tag="VGC69_06675"
     CDS             13701..15662
                     /locus_tag="VGC69_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0295003.1"
                     /translation="MFELFRTHRRWMQVILLLLIVPSFALVGLQSYESFVHREPKLAE
                     VAGQSIERSEFDMAHRNQLEQLRQRLGTQFDPAVVDTPEARRQLLDDLVNHQLLAQVA
                     VDAHFSVSDGTLRDTIAAIPQVQDNGRFSPERYRQVLAAQGLTPTMFEANLRRDLSVG
                     LVLDPVSASAKVPVSVADSIAATLTQQRVIALRDFKADDYRGKVQVSQQDIQAWYDAN
                     KQQLEVPEQVRAQYLVLDEAAATQGVQVKNEDVESYYKQNQARFGQPERRRISHILVQ
                     VPDNATEEQRQAAREHAEDLAAQAAAKPEGFADLAKKFSQDAGSAQSGGDLGWVAPPG
                     LEQAISELSKGQVSGVVASPAGFHVIKVTDFQPSSIKPLAEVRNQIVAEIRKQMAAAR
                     FSDMATQLTKLVYDQRDSLQPSADALGLTLRTAAGITRTGLLPAAQAGAGAASAGPDA
                     VLLDNPRVRQALFSPDVLGDKNNSGVIELAPDTMLAVRVLDAKPAHVPVLADVQADIR
                     ERLTAQRAADAARQAGEDALKALQSATPDAAAGEGFGTALTVSRQTTQGLARPVLEAV
                     MRLTPVQDKPAYAGVSTGADYTLVRLDRVEAGTASDEDRAGLAAQLSSAWGQAEAGAV
                     LQSLRQQYGVKMLPEAEQAIKGDSPADAS"
     gene            complement(15762..>16013)
                     /locus_tag="VGC69_06680"
     CDS             complement(15762..>16013)
                     /locus_tag="VGC69_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY0295004.1"
                     /translation="GARVLVVGMQIPPNYGRDYTQRFMHVFPDVAQAEHAGLVPFLMD
                     GIAADRGLFQADGLHPVEAAQPRLMENVWSQLEPLLKQG"
BASE COUNT         2526 a         5402 c         5542 g         2543 t
ORIGIN      
        1 gcgatgccca gcccgtaatg ggcggaggca agcgccggcg tcgtggcgat ggcttgccgg
       61 taatgcctgg cggcttcgtc gagctcgccg gtttcgcgca gcaggtaggc caggctgagc
      121 gtcgcctggg catggttggg cgcgagcgcc aaggccttgc gcaaggccgc catggcgtct
      181 tgcgtgcgcc ccagccgttg cagggccatg cccttgccgt agcaggcggc gcccgatccg
      241 ggctgcagcg ccagggcctg ttcgtagcag gcgagcgatg gctcgtaggc ctgccgcgcg
      301 cggtgcatcg cgcccagcga cagcaggtat tgcgcattgc gcggggcaag ggtgtgggcc
      361 ttggccagca gcgtcacggc caggtcgatt tctcccagct ggccggccgc attgcccagc
      421 cagtgcaggg cttcgctgtc gttgggattg gcttccagga tctgctggta gatggctacc
      481 gcctgggcca ggcggccggc ccggtggtgc gcgagtgcag tctggaggtt catcgcgcca
      541 tggtacgagc cgccgtattt ctcaaggctg cgcacaagag ccgaaatccc ccggtttccc
      601 ccttcattaa tctgtcgttg aagacggatt tctgtcttta agctgaattt agtcccgatc
      661 gcgtcgcgct agggcttgcg cgccggctgt cccgcacgtg gcaaaccgag cttttgcatg
      721 gcctgctgga tgatcttggc cagcgccgca tagcgcggcc gcacgcggtc gatggggccg
      781 ccgaccagaa tggcgagccg gcggtccggg tagctgatgg gcacggcgat gccgcccagg
      841 tcggcggtga agccgcccac gctttcgaac cagccgcgct tgatgccggc cttcacggcg
      901 gcctcgacct cctgggcgtc ggtgagcgag gtcttggtat aggcgggata gctgatcttg
      961 cgcagcagga tcttgcgttc cgcttcgggc aggagcgaga gcaacgcctg gccggaggcc
     1021 gtggtgtgga tgggcaccag cttgccggaa taggccgagt agcgcacggc ctgccgggat
     1081 tcgatcgtct cgaggaagac cgcatggggg cccatgggcg ccaggatgac ggtggtttcg
     1141 ttcgtggcgc gccagacttc ttcgagcagg tcctgcacga cctggggcag gggctcggcg
     1201 ccggccacgg cttcggccag ggcgcgccag cgcggcgtgg ggtagaagct gccgcggctg
     1261 gcgggctcat acaggaagcc gcgttcggac aaggtgccga gcagattgaa ggtgctggag
     1321 cgcggccatc cgaaatgcag ggagatttcc gcgagcgtgg ccggctgctg ccgcgttgcg
     1381 aagtactcca gcaagtccaa tacgtttgcc gcttgtttga ccagcatgag gcgatttccc
     1441 aaggtgcgtg tttgcgcgga ttttaccggg tcggcgtgtg ttttcccgta gggaatgccg
     1501 aatggtccgg ccttgcggga catacgcttt ttgtttatct tatccacata agtgaatttt
     1561 ttgttcacac atatgaataa aatccaggca aacagaggag acgaaaatgg actttgccct
     1621 gacccaagag caggaggcca tccgcgattc gatcgccaag gtgtgcgagg gcttcggcga
     1681 cgattactgg ctcgagcgcg atagcgacgg ccagtttccc catgcgttct acgatgccct
     1741 ggcccaggct ggctggctgg gcgtctgcat ccccgaggcc tacggcggcg cggggctggg
     1801 catttcgcag gcggcgatca tggcccgcac catcgccgaa tcgggcgccg gcatgtcggg
     1861 cgcttcggcc gtgcacatca atatcttcgg cctgaacccg gtggcggtgt tcggcaccga
     1921 agaacagaag cagcgcatgc tgccgcccat ggcgcagggc cgggacaaga cctgctttgc
     1981 cgtcaccgaa cccaacacgg gcctgaacac cacacagctc aagacccgcg ccgtgcgcaa
     2041 gggcgaccgc tatgtggtca atggccagaa ggtgtggatt tccaccgcgc aggtcgccaa
     2101 caagatgctg ctgctggcgc gcaccacgcc gctggaagcg gtcaagaagc ccaccatggg
     2161 cctgagcctg ttctacacgg acttcgaccg cagccgcgtc gccgtgcacc tgatcgaaaa
     2221 aatgggccgc aaggcggtgg actccaacga actttttttc gaagacttcg acatccccgt
     2281 cgaagaccgc atcggcgagg aaggccgcgg cttcgaatac atcctgcacg gcatgaatcc
     2341 cgaacgcatc ctgatcggct cggaggcggt ggggctgggc agcctggccc tgactcgcgc
     2401 cacggcctat gccaaggagc gcatcgtctt caaccggccc atcggccaga accaggccat
     2461 ccagcatccg ctggcggcga actgggccga gctggaggcc gcctggctga tgctgctcaa
     2521 ggcggcctgg cagtacgaca gcggccagtc ttgcgcagcc ctggccaatg ccggcaagta
     2581 cctggcgggc gaagccggtt tcaacgcctg ccagcaggcc gtcatgacgc acggcggctt
     2641 cggctatgcc aaggaatacc acgtcgagcg ctatctgcgc gaagtgatga ttccccgcat
     2701 cgcgccgatc agcccgcagc tggtgctgag cttcatcgcc gaaaaggtgc tgggcctgcc
     2761 caagtcgtat tgagtcgcca cgcttaggca aggaaggccc ggacatgggt cccagaatca
     2821 tcgaggccgc cgatctgcgc ctgggcgagt tcatccgccc cggcgacacg gtggtgtgcg
     2881 gccagacggc ggccgagccg ctgaccctga cgcaggcgct ggtcgagcag cgcgccgact
     2941 tgtcgggcgc gcgggttttc ctgggcgcgg ttttttcgcg cacgttcgcg cccgagcatg
     3001 ccgatcacct gcggttttct tcttacggcg tcctgggcgc cgcggccgcg ctgggcaggc
     3061 agggcctgat cgatgtgctg ccctgttcgt accacgaact ggaatgccgg tacgccagcg
     3121 gcgcgctggc cgcggacgtc gtgctgctgc aattggcgcc cggcgggggc gatggcccct
     3181 acagcttggg catggccaat gactatgtcg cggcagcggc ccgccgggcg cgcgtggtga
     3241 tcgcggaagt ccatcccgat gtgccctgga cccacggcgc ggcgtggccg cgggacgcgc
     3301 gcatcgacgt tgtcgtgccg gcgacgcggc cgccgctgga tatggcggcc gcggcatgcg
     3361 gaccggtcga ggaggccatc ggccggcatg tggccgcgct gatccccgac ggcgccgtgc
     3421 tgcagaccgg catcggcgcc attcccgatg cggtgctcgc cgccttgcgt tcgcaccgcg
     3481 acctgggcct gcattccggc atggcgggcg accgcatggt cgagctgatc gaggcgggcg
     3541 tcgtcaccaa tgcgcgcaag ccgttcgatg ccggcatcgg cgtggtcaat gtgctggccg
     3601 gcacggccag cacgcgccgc cacgcccatc gcaatccggc gctgatcgtg cggccggccc
     3661 gctacacgca tgcgcaggcc gtgctgtcga ccctgcatgc gctgcacgcc atcaattcgg
     3721 ccctgcaggt cgacctgggc ggccaggtca attcggaaat gctgggcggg gtgccgatgg
     3781 gcggcatcgg tgggctgaac gacttcgtgc gcggcgcgcg ggccgcgccg ggcgggcgct
     3841 cgatcatcgc gctgccgtcc acggcgcgct cgggcgccgt cagccgcatc gtggcccggc
     3901 tggacgacgc ggtcgtcacc gtgccgcgct gcgacgccga cctggtggtc acggagtggg
     3961 gcgtggcgga cctgcgcgcc tgcaccctgg ccgagcgcgc ccgccgcctg acggccgtgg
     4021 cggcgccgca gtttcgcgaa tcgctggaaa aggcctggcg cggcgcccgg caatgaacga
     4081 ccacacagac acacacaagg agacaaaacc atggctgggc tttattacga agaattttcc
     4141 gtggggcagc agttcgatca tccctggacg cgcacggtga ccgagatgga caacaccttg
     4201 ttcagcctcc tgaccatgaa tccgcagccg ctgcacctgg acgcgcattt ttccgccaag
     4261 acggagtggg gacagcggct gttcaacagc ctctacacgc tgggcatcat gatcggcatg
     4321 gcggtgagcg acaccacgct gggcacgacg atcggcaacc tgggcatgac cgaggtgaaa
     4381 tttcccaagc cggtgttcca cggcgacacg ctgcgcgcgc agacccgggt gattgccatg
     4441 cgcgagagca agtcgcgccc caacgcgggc ctggtcgagt tcgaacacac cgcgttcaac
     4501 caggacggca cggtagtggc cacctgcctg cgcaccgcgc tgatgtaccg caagggagcc
     4561 cggccatgaa gtccttcctg ttcgttcccg gcgacagcgg gcgcaagttc gagaaggcct
     4621 cccaaggcgc ggccgacgcg ctcatcctcg acctggagga ttcggtggcc gccgacgcca
     4681 agccccaggc caggcaggcc acggcggcca tgctgcgcca gccgcgcggc agccagcgcc
     4741 gctatgtgcg ggtcaatgcg ctcgacaccg gcatgacgct ggccgacctg gccgccgtca
     4801 tgccggcggc gcccgacggc atcgtgctgc ccaaatgccg gggcggcgac gatgtgcggc
     4861 tgctggccca ttacctggat gccttcgagg ccgcgcacgg actggcgggc ggcggcaccc
     4921 gcatcatcgc catcgccacg gaaacggccg aatccgtttt cggactgggc agctaccgcg
     4981 gctgcagcga gcggctgtgg ggcctgatgt ggggcgcgga agacctgtcc gcgtcgctgg
     5041 cctcgagcgc caatcgcgtc gacggggcct ataccggccc ctacgtcatg gcgcgcaacc
     5101 tgtgcctgat gggcgcggcc gccgcggagg tggccgcgat cgacacggtc tacgtggata
     5161 tcgaggacgt cgcgggcctg ggcgaggagg cccggcaggc aaggcgcgac ggcttcgcgg
     5221 ccaaggccgt gatccatccc aggcacgtcg acgccgtcaa ccaggccttc acgcccacgc
     5281 cggaagaaac cgcgtgggcc aggaccatcg tcgcggcttt cgcggccgcg ccgggcgccg
     5341 gcgtgttcaa gctgaacggc cagatgatag acaagccgca cctgcgccgg gcgcaggcca
     5401 ttttggggca gtgaccatgg gaccgctagc gggcttcaag attctggaca tgaccgcggt
     5461 gctgatggga ccgtcggcca cgcaggtgct gggcgacatg ggcgccgaag tgatcaaggt
     5521 ggagtcgccc gacggcgaca tggtgcgcca gatcggaccg tcgcgccacc ccggcatggg
     5581 gccgatcttc atgcacgtca accgcagcaa gcgcagcatc gtgatcgacc tcaagcaggc
     5641 gcagggccgc gcgctgctct tgcgcatggc ggcccgggcc gatgtgctgg tctacaacat
     5701 gcggccgcag gccatggcca ggctgggcct gacctacgag gcgctggccg gggtcaatcc
     5761 cggcctggtc tatgccggcc tgttcggctt cggccaggac ggcccctatg ccgaccggcc
     5821 cgcctacgac gacctgatcc agggcgcggc gaccattccc tacctgttcg tgagggccaa
     5881 cggcggcgag ccgcgctatg cgcccagcgc catcgccgac cgcatcgtgg ggctgtcggc
     5941 cgtgggtgcc atcctcgcgg ccctgctcga gcgcggcagg acggggctgg gccagcgcgt
     6001 ggacgtgccc atgctcgaaa ccatggtcgg cttcgtcctg gcggaccacc tgggcggcct
     6061 gaccttcgag ccgcccctgg accgcggcgg ctatgcgcgc cagttgtcgc ccaatcgcaa
     6121 gccctaccgc accagggacg gctacgtctg cgccatggtc tacaccgaca agcaatggcg
     6181 cagtttcacc caggccatcg gcgtgccgga cctgatggac caggacccgc gctttgccga
     6241 cttcaaggcg cgcacggcga acatcgactt cgtctatacc gagctgaccc gctggttcct
     6301 ggaacgcacg tcgcgggagt ggctggcgct gctggagcgg gccgacattc ccagcatgcc
     6361 catgcacgac ctggaaagcg tgctggacga cctgcacctg aaggcggtcg gcttctttcc
     6421 ggtggtggcg catcccaccg aaggggcggt gcgcaccctg cgggtggcgg ccagctggtc
     6481 gcgcacgccg gcgcggcccg aacgcctgcc gccggtgctg ggccagcata cggtggaggt
     6541 gctgcgcgaa ctgggcctgg acgacgcgga gatcggcgcg ctgcgcgaga gcggggcggt
     6601 cgtgcaggcc tagctgtcgg ggcggggtgg aacggatcgc cgcaggcgta agatggaact
     6661 ctccttcttc gatgcccccg gagccgcgat atgccgcagc aaccccgcct ggactacgtt
     6721 acctgcacca gccccgccgg cctgcaccgc atggcctatt gggaatgggg cgatccggcc
     6781 aacgaccagg tgctggtgtg cgtgcacggc ctgacccgca ccggccgcga cttcgacgag
     6841 ctggcgcgcg cgctggcggg cgatttccgg gtggtctgcc ccgacgtggt gggacgcggc
     6901 gcctcggact ggctgctcaa tccgtccttt tatacggtgc cgcagtacgt gtcggacatg
     6961 gtcaccctga tcgcgcgcct gcgtcccgcg tcgctgaatt gggtgggcac gtcgatgggc
     7021 ggcctgatcg gcctgggcgt ggccggcacg gccgccatga cggcgatgat gcgcgccgcc
     7081 gatatccgcc cgggcgcgca gtccgaggat ctgcccgatg gggcatccgc cccgggccgc
     7141 atccgcttcg accgcctggt gctcaacgac gtggggccgc ggctggagct gtcggccctg
     7201 ggccgcatcg ggcagtacgt gggacagccg gcctggcacg ccacctttgc cgaggcggtg
     7261 cgggcggtgc gcgaaacggc ggcgaccttc ggcccgcata ccgacgaaca atgggaaacg
     7321 ctcacgcgcc acgtctaccg ccagcgcgat ggccgctggg tcaagcatta cgacctgggc
     7381 ctggccgtgc cgctggtcgg gcagacgccc gacgcctacg cgacgggcga gcaattgctg
     7441 tggcaggcct acgatgcgct ggactgtccg gtgctgatcg tgcgcggcgc cgagtccgac
     7501 ctgctgtcgg cggcaacggt ggccgagatg cgcgcgcgca acgcgcgggc cacttcggtc
     7561 gaattcgccg gggtggggca cgcgccgacc ctcatgacgg cggcgcagat cgcgccggtg
     7621 gcggccttcc tgcgctcttc tctacaatag acccatggac accgcattgc tcaacatcga
     7681 cctgcattgc cattcctccg tgtccgacgg ccagctgccg ccggccgcgg tggcgcgccg
     7741 tgcccagggc aacggcgtgg acgtgtgggc gctgaccgac cacgacgagg tcgacggcct
     7801 ggccgaagcg gggcggacgg cgcgcgagct gggcatgcgc ttcatcacgg gggtcgaggt
     7861 gtcggtgacc tgggccaatc agacggtgca catcgtcggc ctgaatttcg accctgctga
     7921 ccagaccttg atcgaaggcc tgcgcgccac ccgttctggg cgcggcgagc gggcgcgccg
     7981 catgggcgaa cgcctggccg cgctgggcat gccgggcgcc ttcgagggcg cgctgacttt
     8041 cgccggcaac cccgagctca tcagccgcac gcatttcgcg cgctacctgg cctcggccgg
     8101 gtactgcccc gacgtgcaga cggtgttcaa caaatacctg cacgacggcg gtccgggcca
     8161 tgagcccgtg cagtgggcca cgctggcgca ggccgtgtcg tggatatgcg gcgcgggcgg
     8221 ccgggcggtc gtggcgcatc cgggacgcta caagtacacg ccggtgcagt tcgccgcgct
     8281 gttcgacgag ttcatccagc tgggcggcga aggcgtggag gtcgtcaccg gcagccacag
     8341 ccccgaggac gcccgcaagt acgcgggcga ggcgcgccgc tacggcctgc tggcctcgcg
     8401 cggctccgat ttccacagcg tgcaggacag ccggatcgac ctcggcatgc tgcccatgct
     8461 gccgccggac ctcaagccgg tctggcacga ttggttttga catgaacaag gctttcgtca
     8521 aagagtccga gcacgacgac gaggacgata ttcccgaagc ccaggccctg cccgcgggca
     8581 cgcgcaacta catgacgccg cagggctatg cccagctgcg cgacgagctg gcgcagctga
     8641 tgaacgtgga gcggcccgcc gtcgtgcagg tggtgtcctg ggcggcttcc aacggcgacc
     8701 gttccgagaa cggcgattac ctgtatggca agaagcgctt gcgcgagatc gaccgccgca
     8761 tgcgctttct gaccaagcgg ctggacatcg ccgaagtggt cgatccgggc ctgcagccca
     8821 accgcgacca ggtgttcttc ggcgcgaccg tggtctacct ggaccgcgcc ggcgtcgagc
     8881 gcacggtgac catcgtgggc gtggacgagg ccgagccgct ggccggccgc atcagctgga
     8941 tatcgccggt ggcgcgggcg ctgatcaagg cgcgcgaggg cgacaccatc accctgcgca
     9001 cgccgggcgg cgtggacgag ctggagatcc tcgcggtgca ttaccccgag ccgctgccta
     9061 agtaactaag caaataacgc gtttgtcgcc atgacagcct ggcgccgtcc gtataattgt
     9121 ccgccatgag atcgcaccgc gcccgattcc ggaccgcctg gcttggcctc ttggccatgt
     9181 tcctggtggt gttcgcgccg ctggtcagcc agtcgctggc ggccacgcgg gcggcccagg
     9241 cgcaggcgat cgaactcgag ctgtgctcgg tcgacgcgca cgccgcgccg gcgcatcccg
     9301 atcatgcggg catgcacggc gacttgctgg ccgcctgcgg ctattgcgac ttcctggccg
     9361 gccatgccgc cttgcctgcc gtaccgccgg ccgcgctggc gctgatcgtg ctggtactca
     9421 ttgcgatggt gaccatgccg tcgctgcgcc atatcgcgtt tggcgctttc ccctcgggcc
     9481 gcccacgggc cccgcccatt ctttctcgcc tggctgtgta aatcgccgcg ccccatgcgg
     9541 ggcgccttgg cgcatcgcgc agagagcgga tgcgcctgtc cagtttcgag gaagttttca
     9601 tgcgtacggt tttttcgcat ggcgcgcacg cgtcgatgca cggcttgcac gggcgcggcc
     9661 gcgccatgct ggccatggcc gcctggtgcc tgatgcaagg ccaggcccgg gcggcgggca
     9721 acaatcccca ggagctccag accgtccagg tgacgggctc ggcgccttcc tatgccgagc
     9781 agaacctgct gccgcagacg acggaaagcg tcacggcccc gcagattgcc gagacggtca
     9841 atgccatggg cgtggaggac gccctcaaat acctgccgga cgtgctggtg cgcaagcgct
     9901 acatcggcga tacccaggcg cccatggcga cccgcaccac gggcatcaat gccagcgcgc
     9961 gcagcctgct ctatgccgac ggcattctgc tttcttccct gatcaacaac aacaaccaga
    10021 atggctcgcc ccagtggttc atggtgtcgc cttcggaaat cgagcggatc gacgtgatgt
    10081 acggcccgta cgccgcggaa ttccccggca attcctacgg cgcggtgacg cgcatcgcca
    10141 cgcgcatgcc ggaccggttc accggcagcg tggacgtcat cggttccgtc caggcattcg
    10201 gccagtacgg cacgcacgtc agcgaaggcg cccagcaata cagcgcgacg ctgggcgacc
    10261 gcagcggcca gttttcctgg cgcctgagcg cccgccacct cgacagcgac tcgcagccga
    10321 tcacctacgg cacgctgacc cagtcgcaga cgcccgccgg cgcgggcctg cccttcgtgt
    10381 ccggcgcgtg ggccggcacg aaccgcgcgg gcagccccat gcagatcatc ggcgcgggca
    10441 atttcaccca taccagccag gacaacgcca cgctcaagct ggcctacgat ttcacgcctg
    10501 acctgaccgc gacctatacg ctgggctatt ggcagaacca tgcccggacc tatgcccgga
    10561 gctatctgtc cagcgccgac ggcacgccct actacggcgc cgccagcggc aatgtgaacc
    10621 tgggcggcta tgcctatggc gccagcgcca tcgccgggca gttctccgac ggccaggtcg
    10681 agcaggagca cctgatgcag agcctggagg tgggcacgca ctcggacggc ttgttcgact
    10741 ggcaagcggc gctgagcaat atgcgctacc tgcaggactt gagcaccacc tccacgggcg
    10801 tgttcccgca ggcgcagaac ggcggcgccg gccgcatcac cgacatgggc ggaaccggct
    10861 ggaccaccct ggacctcaag ggcacctggc gcccgcaagg cgagtcgggc gcgcatatcg
    10921 tcagcgccgg cctgcacgac gaccggttca cgctcgacag cccgacctat acgaccacgg
    10981 actggctggg cggcggcgag ggcagcctct acagcaatgc cctgggcaag accgagacgc
    11041 gcgcgctatg gctgcaggac acctggcgcc tggcgccgga ctggacggcg acgctgggcg
    11101 gacgctacga atggtggcag gcttttgacg gctacaacta cgcgatcggc agcaatgggg
    11161 cgggcgtgcc cattgcccag ccgggcgtga ggcagtccgg cctgtcgccc aaggcctcgc
    11221 tggcgtggca gctcgacgat gcctggtcgc tgacggggtc gttcggcaag gcgctgcgct
    11281 ttcccacggt gggcgagctc tatcaaagcg tgcagaccgg ctcgacctat ctgcaggcca
    11341 atccctatct caagcccgag cgcgtgctgt cgagcgaact ggcgctggcg cgcaatacgg
    11401 acgacagcca ctggcgcctg tcgctgttca acgaagacgc ggccgatgcc ctgatctcgc
    11461 agaccgcctt tcttcccggc tttgccacgc cggtgtcgta cacgcagaac gtgggccgga
    11521 cgcggcagac cggggcggaa ctggcctggc ggcaggacca cgtgctctgg cacggcctgt
    11581 cgctgtcggg caacctgacc tatgtcgatg cgcgcatcct gtccaacgac ggtttcgcct
    11641 ccgccacggg cacgacctcg gtgggcaagc gcgtgcccta cgtgccgacc tggcgcgcca
    11701 cgctggcggc cacctaccgg ccggacgaca aatggtccta tacgctggcg gggcgcttca
    11761 gctcgcgggt ctatgccagc gtggacaaca gcgacgtcaa cgcggcggcg ttcacgggct
    11821 tccagagctt cctggtcttc gatgcccggg tgcattacca gatggacaag cactggagca
    11881 tggcgctggg cgtggacaac ctgggcaaca gcaagtactt cctgtaccac cccttcgagc
    11941 agcgcaccta ttacgccgag gtcaagtacg ccttctaggg gttctcgcgg tagtccagcg
    12001 tgtagccggc ccggtcgtcc agggccagtt cgcgcgccag gtagggcagg gcggtcttga
    12061 gcgccgcctg cagcgtccag ggcgggttga ccaggaacat gccgctgccg tgcaggccga
    12121 agccgtcggc ggcgggcttt tgcacggtga gcgtggcatg cagccagctc ttgacctgca
    12181 ggtgcggcag ctggcgggcc agttcgcccg cctcgacccg ctgcaccagc ggataccaca
    12241 cggcataggt gccggtggca aagcgcttga tgccttcctt gacggcgttg aacgcgcggc
    12301 ggtaatcgcg cttgtcttca tacgacgggt cgatcagcac cagcccgcgc cgcgtgggcg
    12361 ggggcagcag cgatttcatg ccttcgaagc cgtcggcgtc gaacaccgtg gtctgccgca
    12421 gcggaccgcg gccgcgcttt tccaggttgc gcaccagcac ctccgattcg ctggggtgca
    12481 gttcgaagag gcgcaggcgg tccttgtcgc gcagggcgtc cagcgtcagc cagggcgagc
    12541 ccgggtagta gcgcagctgg ccgtcggggt tgtattggcg gatgtgggcg acatagtcgg
    12601 ccagcagttc gggcaggtcg gccttgtccc acagctgggc gatgccgccg gcgtactcgg
    12661 cgttcttgga cgaccagtcg ctgtcgagct ggtacaggcc ggctcccgca tgggtgtcga
    12721 tcacccaata gggcgcgtcc ttgcggttca ggtaatccag cgtgtgcagg aggatggcgt
    12781 gcttgagtac gtcggcatgg ttgccggcgt ggaaggcgtg gcggtagctg aacattaggc
    12841 ggtgggctgg atcagcgtca gttgatcggt gaagatggcg tccacgcccc acgaaaacag
    12901 caggcgcgcg cgatcggggt cgttgactgt ccaggcggcc aggcggtagc cggccgcgtg
    12961 cacggcggcc gccagctcgg gcgtggcgtc tttctggttg atgttcaggc ccacgcaggc
    13021 gtattggcgc aggcgggccg gccagtcgtc ggggacggtt tcgaccagca gggcgcgggg
    13081 cagctcgggc gcggccgcct gcgcggccgc cagggcttct tcggaaaacg acgacaagag
    13141 gggccgcttg tgcacgcccg cccagaattg ctgcgcgagc ctggccacgg cctcgccggt
    13201 ttcggcgtcg cggccggggc agggcttgat ctcgacgttg caggcgatgc cgttggccac
    13261 ggcgtagcgg gccacggcgg cgaaggtggg caccggctcg ccggcgaagg ccgggctgtg
    13321 ccagccgccg gcatccaact gagccagctc ggcgtaggtt ttttccctgg ccgggccgtg
    13381 gccgtcggtg gtgcggtcga cgacgtcgtc gtgcagcagc acggccacgt tgtcgcggct
    13441 caatttcacg tcgaactcga ccatgcggta gccgcgccgc ttgccggcgc gcatggcggc
    13501 cagggtgttt tcgggcgcca gccggccgcc gccccggtgg gcgatgtggg cggggtaggg
    13561 ccaaggggcg aggggcgtgg tcatggtatc cggacggtgg cggctgggat gaggaaaccg
    13621 ccttggaaaa acggtaaact caggggttta gccgtgatta tcccaaaaac tgcaagctcc
    13681 aagtagccaa tcaggattcc atgttcgagt tattccgtac gcatcgccgc tggatgcagg
    13741 tcattctgct gctcttgatc gtgccgtctt tcgccctggt cggcctccag agctatgaaa
    13801 gcttcgtgca tcgcgagccc aagctggccg aagtggccgg ccagtccatc gagcgctccg
    13861 aattcgacat ggcgcaccgc aatcagctcg agcagctgcg ccagcgcctg ggcacgcagt
    13921 tcgacccggc cgtggtcgat acgcccgagg cccgccgcca gctgctcgac gacctggtca
    13981 accaccagtt gctggcgcaa gtggccgtcg acgcgcattt ctcggtgtcc gacggcacgc
    14041 tgcgcgacac catcgccgcc attccccagg tgcaggacaa cggccgtttc tcgcccgaac
    14101 gctatcgcca ggtgctggcc gcccagggcc tgacgcccac gatgttcgag gccaatctgc
    14161 gccgcgacct gtccgtgggc ctggtgctcg accccgtctc ggccagcgcc aaggtgccgg
    14221 tttcggtcgc cgattcgatc gccgccacgc tgacgcagca gcgcgtgatc gcgctgcgcg
    14281 acttcaaggc cgacgactac cgcggcaagg tgcaggtgtc gcagcaggac atccaggcct
    14341 ggtacgacgc caacaagcag cagctcgaag tgcccgagca ggtgcgcgcg caatacctgg
    14401 tgctcgacga ggccgccgcc acgcagggcg tgcaggtcaa gaacgaagac gtcgaaagct
    14461 attacaagca gaaccaggcc cgcttcggcc agcccgagcg ccgccgcatc agccacatcc
    14521 tggtccaggt gccggacaac gccaccgaag agcagcgcca ggccgcgcgg gagcatgccg
    14581 aagatctggc tgcccaggcc gccgccaagc ccgagggctt cgccgacctg gccaagaaat
    14641 tctcgcagga cgccggttcc gcccagtcgg gcggcgacct gggctgggtg gccccgccgg
    14701 gcctggagca ggccatctcc gaactgtcca aggggcaggt gtcgggcgtg gtggcgagcc
    14761 ccgccggctt tcacgtgatc aaggtcaccg acttccagcc cagctccatc aaacccctgg
    14821 ccgaggtgcg caaccagatc gtcgccgaga tccgcaagca gatggccgct gcgcgcttct
    14881 ccgacatggc cacccagctc accaagctgg tctacgacca gcgcgacagc ctgcagccct
    14941 ccgccgacgc gctgggcttg acgctgcgca ccgccgccgg cattacccgt accggcttgc
    15001 tgcccgccgc ccaggcgggc gcgggcgcgg cctcggccgg ccctgacgcc gtgctgctgg
    15061 acaatccccg cgtgcgccag gcgcttttct cgcccgacgt gctgggcgac aagaacaact
    15121 ccggcgtgat cgaactcgcg cccgacacca tgctggccgt gcgcgtgctg gacgcgaagc
    15181 cggcccatgt ccccgtcctg gccgacgtgc aggcggacat ccgcgaacgc ctgacggcgc
    15241 agcgcgccgc cgatgccgcg cgccaggctg gcgaagacgc cctcaaagcg ctgcagtcgg
    15301 cgacgcccga cgccgccgcg ggcgaaggct tcggcacggc cctgaccgtg tcgcgccaga
    15361 ccacccaggg cctggcccgc ccggtgctcg aggccgtcat gcggctcacg ccggtgcagg
    15421 acaagcccgc ctatgccggg gtgtcgacgg gtgccgacta caccttggtg cgcctggacc
    15481 gcgtcgaggc cggcacggcg agcgatgaag accgcgccgg cctggccgcg cagctgtctt
    15541 cggcctgggg ccaggccgaa gcgggcgccg tgctgcagtc gctgcgccag caatacggcg
    15601 tcaagatgct gcccgaggcc gaacaggcca tcaagggcga cagcccggcc gacgcatcgt
    15661 aatcggatcg ggaagcgctg gccgctcccc gcgaaagacg ggcgcctggg agatctctct
    15721 cccggacgcc cgtctttttt cattgcatca ttgaattttg ctcacccttg cttgagcagg
    15781 ggctccagtt gcgaccagac gttttccatc aagcgaggct gggccgcttc caccggatgc
    15841 aggccgtcgg cctggaagag gccgcggtcg gccgcgatgc cgtccatcag gaagggcacc
    15901 agcccggcat gctcggcctg cgccacgtcg ggaaagacat gcatgaaacg ctgcgtgtaa
    15961 tcgcgcccgt agttgggtgg aatctgcatg ccgaccacca gcacgcgggc gcc
//
LOCUS       DASWPO010000115         6690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2792653, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000115 DASWPO010000000
VERSION     DASWPO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2792653"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VGC69_06685"
     CDS             complement(<1..409)
                     /locus_tag="VGC69_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295005.1"
                     /translation="MHAKWLALPFAMLLAMSAQAADKVKLGFISTLSGPLSGPSTEIR
                     DGLRLALEETGNRIGGLPVEVVEGDDEANPSVGRQLADKFLKRDKVDFMTGVIYSNVM
                     QAIAAPTFAAKTFYISAAAGPASLAGAQCNPYFF"
     gene            complement(478..993)
                     /locus_tag="VGC69_06690"
     CDS             complement(478..993)
                     /locus_tag="VGC69_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4863 family protein"
                     /protein_id="HEY0295006.1"
                     /translation="MNTPSELPTGPSKAAMMQAVRELLDFIGDRPLGPELEALLNERY
                     GAHTEAYARLLTLLRRGMDEGWSCYAEIDGPDYRRGRIAQASEETHGFTVESGCLRDV
                     LGNYHRHPLGEINMIGPVDETGRFCGSGAGWKVFPPDSSHFPTVTGGVVTMLFFLPDG
                     LIEYKDPPAVG"
     gene            1142..2134
                     /locus_tag="VGC69_06695"
     CDS             1142..2134
                     /locus_tag="VGC69_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295007.1"
                     /translation="MDLISSLVAFLRVAEAGSFSVVASERGVTQPAISRQVRALEEHV
                     GMRLVQRSTAGVALTEEGRNLLTPARNLVEAAEALSLAANPVQARPMGKVRLSLPAPL
                     ALHICLHIRALLDSHPNLNVDMVVRDSTSNLIEEGLDLEVRIGPIDDSSLISRRVGMT
                     SAFLVAAPSYMADKPLPCSPQDLPNYDCVVYSRWGKGDTWRFEAPEGEVSVSVPCRLR
                     ANNASAVHHAVLAGNGIAILSHLMVADDLNAGRLISLLPECLPARLPLYIVYPSRHNL
                     PLRTRVVIEFIADLLKNDPSMRDTPVWPVVPSPAAGAPSARPSQVAFHAGPPAG"
     gene            complement(2166..3362)
                     /locus_tag="VGC69_06700"
     CDS             complement(2166..3362)
                     /locus_tag="VGC69_06700"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0295008.1"
                     /translation="MAAQQILSGIRVLELGQLIAAPFAAKTLADFGAQVVKIEPPGQG
                     DPLRKWRLLHEGTSVWWEAQSRSKQSVCVDLRKPEGQDIVRTLAAQADVLIENFRPGT
                     LEKWGLSWEALRAANPRLVMLRISGYGQTGPKAGEPGFAAIGEAMAGLRYLNGEPGRA
                     PVRAGISIGDTVAGLYGALGVLLALYQRDARGGQGQVIDAALFESIFSLTESLLPEYS
                     VFGAVREPAGAALPGIAPSNAYLCRDGYVLIAGNGDGIFGRLMDKIGRPDLGRDPGLA
                     QNDGRARRAAELDAAIGGWTGQRTIAEVLDAMREADVPSGRIYTVADIAGDPHYRARH
                     AIAKVTAASGIEVEMPAVFPLLSETPGAIAHRAPTLGEHTDAVLAAAGFDAGQRETLR
                     ALGVIA"
     gene            complement(3435..4898)
                     /locus_tag="VGC69_06705"
     CDS             complement(3435..4898)
                     /locus_tag="VGC69_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0295009.1"
                     /translation="MAFQHAYDLQERGQYRESVASYRQVLAKRPGHAGGHNNLGLALM
                     ALGELDEAIASFRQAAALQPNYAEAHNNMGVAYKRMAKAQEALDCYAAALKCKPDYAQ
                     ALLNVGTIYYDLKEYDEALVWYQAALEVDPGQIEVHMNIASIFEAQGRFEDVKKHREV
                     VYGRKAVYIDRAAEPRLTALVLWGAGLGNIPIHYLLPQKTITRIGCIIEYAQPRELEN
                     LPGYDLVFNAIGDPDVMDGVEEPMARFLRTCDKPLINRPEAVARTARHLIPDLLGKLD
                     NVVVPRTSRVETARLHDTLRAIQDGHFPILLRPINSHGGDHLVKLESKADIAGLVPWR
                     APYYYLTDYADYASADGGFRKYGVIFIDRQPHAYHMAISSNWVVHHNTAGMQHEEWKR
                     REEEAFLADPRQAIGAKAMAAIEAIGRALDLDYGGVDFSILPDGRVLVFEANATMLTH
                     PEEESGLYHYKNPYVRRIFDAFDAMLARRMAEVHATA"
     gene            complement(4924..6036)
                     /locus_tag="VGC69_06710"
     CDS             complement(4924..6036)
                     /locus_tag="VGC69_06710"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY0295010.1"
                     /translation="MLETARLRNARNFSGGPGALPESVLQQLHESMLCVPEVGMSVLG
                     ISHRSDWFAAVVAEAERNVRALLNLPDGFHVLFLQGGATQQFFTLPMALARPPAPPPE
                     YLLTGYWSGKALAAATQRHRINVAWSGKDDGFRILPRMADLALSADAPYFHYISNETV
                     EGIQFQEIMGRDDVPRVCDMSSDFLSRPCEAARYSLLYAHAQKNLGPAGVTVVVVRDE
                     MLGKLPADLPEFLDYRIHATAHSNYNTPPVFAIYAVMLATRWLTREVGGLDEMGAINR
                     RKAALLYQAIDDSEGYYKGRADAGHRSTMNVAFNMATPELERRFLQASSEAGFSGLNG
                     HRAIGGMRASLYNGVTIEAAEALVGFMHDFRKRHAA"
     gene            complement(6039..>6690)
                     /locus_tag="VGC69_06715"
     CDS             complement(6039..>6690)
                     /locus_tag="VGC69_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEY0295011.1"
                     /translation="VSPALARTDRLALDELARGVAGLGDVLPEAVFPILVRPVDSNAG
                     EGLGKIDAPAELAAYLGATPGNDFFVSQFVDYRSADGRYRKYRVILMQGKAYLAHLGI
                     SSRWMVHYLNADMQDNAANRAEEGEAMQGFDQGFGERHAPAFRVFHERVGLDYLVMDC
                     SETPDGRLLVFEADTCGVVHDMDPEDVYPYKKAQMHKVFAAFQSMLAAQAGRETGA"
BASE COUNT         1049 a         2218 c         2244 g         1179 t
ORIGIN      
        1 cgaagaaata cggattgcac tgggcgcccg caagcgacgc gggacccgcc gcggcgctga
       61 tgtagaacgt cttcgcggcg aaggtgggcg ctgcgatcgc ctgcatcacg ttcgagtaga
      121 tcacaccggt catgaagtcg accttgtcgc gctttagaaa cttgtcggcc agctgcctgc
      181 cgacactcgg gttggcctcg tcatcgccct ccacgacttc gacgggcagg ccgcctatcc
      241 ggttgccggt ctcctccaga gccagccgca agccgtcgcg aatctcggtg ctcggcccgg
      301 agagcgggcc cgacagcgta ctgataaacc ctagcttcac cttgtctgct gcctgcgcgg
      361 acatcgccaa cagcattgcg aacggcaatg cgagccattt tgcgtgcatg acggataccc
      421 cttttcgata tggaatgcgt ggtgggagcg attgaacgcg gcggagtccc gccgcgttca
      481 gccgacggcg ggcggatcct tatattcgat caggccatcg ggcaggaaaa acagcatggt
      541 gacgacgccg cccgtgacgg tggggaaatg cgaactgtcg ggcgggaaaa ccttccatcc
      601 ggcgccgctg ccgcagaagc ggcccgtttc gtcgactgga ccgatcatat tgatttcacc
      661 caggggatgg cggtgataat tgcccagcac gtcgcgcagg cagccgcttt ccaccgtgaa
      721 gccgtgcgtt tcttcggaag cctgcgcgat cctgccgcgc cggtaatcgg ggccgtcgat
      781 ctcggcatag caggaccatc cctcgtccat gcctcggcgc agcagcgtca gcagccgggc
      841 ataggcctcg gtgtgggcgc catagcgctc gttgagcagg gcctcgagtt ccgggccgag
      901 cggacggtct ccgatgaagt ccagcaattc cctgaccgcc tgcatcatgg cggctttgct
      961 gggaccggtg ggcaattccg acggcgtgtt catgggcgct acctgtgttg atccggacgc
     1021 ttgtttcgat caaatcctac gggtattccc aggaggacac tagaagggcc aaccgtataa
     1081 tcattaataa gaaaagtatg aaagctcggt ccgctgttgc atatattctg caaagttcgt
     1141 catggatctg atttcctcgc tcgttgcttt cctgcgtgtc gccgaggccg gttcgttctc
     1201 ggtggtcgca agcgagcgtg gcgttaccca gcccgccatc tcgcgacagg tgcgggcgct
     1261 cgaggaacac gtcggcatgc gcctggtcca gcgcagcact gccggcgtcg ccctgacgga
     1321 agaaggccgc aacctgctga ccccggcgcg caatctggtc gaggcggccg aggccctcag
     1381 cctggccgcc aatccggtcc aggcccgtcc catgggcaag gtgcggctga gcctgccggc
     1441 gccgctggct ttgcatatct gcctgcacat acgcgccctg ctcgacagcc accccaacct
     1501 gaacgtggac atggtggtgc gcgacagcac cagcaacctg atcgaggaag gcctggacct
     1561 ggaagtgcgc attggtccca tcgacgacag ctcgctgatc tcgcgccggg tagggatgac
     1621 ttcggcgttt ctcgtggccg cgccgtccta catggccgac aagcccctgc cctgctcccc
     1681 ccaggacctg cccaactatg actgcgtggt ctacagccga tggggcaaag gcgatacctg
     1741 gcggttcgag gcgccagagg gggaggtatc ggtcagcgtg ccctgccggc tgcgcgccaa
     1801 caatgcgtcg gccgtgcatc atgccgtgct ggcgggcaac ggcatcgcca tcctgtctca
     1861 cctgatggtg gcggacgacc tgaacgccgg gcgattgata tcgctattgc cggagtgttt
     1921 gccggcgagg ctgccgctct acatcgtcta tccctcgcgc cacaacctgc ccctgcgcac
     1981 gcgcgtggtg atcgaattca ttgccgacct gctcaagaac gatccgtcga tgcgcgacac
     2041 tccggtttgg cccgttgtgc cttcccccgc tgccggtgca ccgtccgcac gcccgtcgca
     2101 ggtcgccttc cacgccggac cgccggcggg gtgagcgcca gcgcatagcc cagccgcgcg
     2161 ccccctcacg caatcacccc cagcgcccgc agcgtctccc gctgcccggc atcgaacccc
     2221 gccgccgcca gcaccgcgtc ggtatgctcg cccagcgtgg gcgcgcggtg cgcgatggcg
     2281 cccggggttt cggacagcag gggaaagacc gccggcatct cgacctcgat gccgctggcc
     2341 gcggtgacct tggcgatggc atgacgcgcg cggtagtgcg ggtcgccggc gatgtcggcc
     2401 accgtataga tgcgccccga cggcacgtcc gcctcgcgca tggcgtcgag cacctcggca
     2461 atcgtgcgct gccccgtcca gccgccgatg gcggcgtcga gctcggcggc gcgcctggcg
     2521 cggccgtcgt tctgcgccag accgggatcg cgccccaggt ctggccgccc tatcttgtcc
     2581 atgagccggc cgaagatgcc gtcgccgttg ccggcgatga gcacatagcc gtcccggcat
     2641 agataggcat tggacggcgc gatgcccggc aaggcggcgc ccgccggctc gcgcacggcg
     2701 ccgaacaccg agtattcggg cagcaggctt tcggtcaggc tgaagatgct ttcgaacaag
     2761 gcggcatcga tcacctggcc ctggccgccg cgcgcatcgc gctggtagag ggccagcagc
     2821 acgcccaatg cgccgtacag gccggccacg gtgtcgccga tggaaatgcc ggcgcgcacc
     2881 ggcgcccggc ccggttcgcc gttgagatag cgcaggcccg ccatggcctc gccgatggcg
     2941 gcaaagcccg gctcgccggc cttggggccc gtctgcccgt agcccgagat gcgcagcatg
     3001 acgaggcgcg ggttggcggc gcgcagggct tcccacgaaa ggccccattt ttccagggtg
     3061 ccgggccgga agttctcgat cagcacgtcg gcctgggccg ccagggtgcg aacgatgtcc
     3121 tgcccttcgg gtttgcgcag gtccacgcac accgactgct tgctgcgcga ctgggcctcc
     3181 caccacaccg aggtcccttc gtgcagcagg cgccatttgc gcagggggtc gccctggccg
     3241 ggcggttcga tcttgacgac ctgggcgccg aaatcggcca gggtcttggc ggcgaagggg
     3301 gccgcgatca attggccgag ttccagcacg cggatgccgg acaatatctg ctgcgctgcc
     3361 atgtctcctc cagtcggcgt tgtcgtagcg tgggaggaaa cagtctacac gcggggtccg
     3421 gcccgcgccg gcgctcaggc ggtggcgtgg acctcggcca tgcgccgtgc cagcatggcg
     3481 tcgaacgcgt cgaatatccg ccgcacgtac gggttcttgt agtggtagag gccgctctcc
     3541 tcttccggat gcgtcagcat ggtggcattg gcctcgaaca ccagcacccg gccgtccggc
     3601 aggatggaga aatccacgcc gccgtagtcg agatccagcg cgcgcccgat ggcttcgatg
     3661 gccgccatgg ccttggcgcc gatggcctgc cgcgggtcgg cgagaaaggc ctcttcttcg
     3721 cggcgcttcc attcttcgtg ctgcatgccg gcggtattgt ggtgcaccac ccagttgctg
     3781 ctgatcgcca tgtgataggc atggggctgg cggtcgatga agatgacgcc gtatttgcgg
     3841 aatcccccgt ccgccgacgc gtaatcggcg tagtccgtca ggtagtaata gggcgcccgc
     3901 cacggcacca ggccggcgat gtccgccttc gattcgagct tgaccaggtg atcgccgccg
     3961 tgcgagttga tgggccgcag caggatgggg aaatgcccgt cctggatggc gcgcagcgtg
     4021 tcgtgcagcc gcgcggtctc gacgcggctc gtgcggggca cgacgacgtt atccagcttg
     4081 ccgagcagat ccggaatcag atgccgcgcc gtgcgcgcca cggcctcggg ccggttgatc
     4141 aagggcttgt cgcaagtccg caggaagcgc gccatgggct cctcgacgcc atccatgacg
     4201 tccgggtcgc cgatggcgtt gaagaccaga tcgtagccgg gcaggttctc cagttcccgg
     4261 ggctgggcgt actcgatgat gcagccgatg cgggtgatgg tcttctgcgg aagcaggtag
     4321 tggatgggaa tgttgcccag cccggcgccc cacagcacca gcgccgtgag cctcggttcg
     4381 gccgcccggt cgatgtacac cgccttgcgg ccgtagacga cctcgcgatg cttcttgacg
     4441 tcctcgaagc ggccctgcgc ttcgaagatg gatgcgatgt tcatgtggac ttcgatctgg
     4501 cccgggtcga cttccagcgc ggcctggtac cagaccagcg cttcgtcgta ttccttgagg
     4561 tcgtagtaga tcgtgccgac gttcagcagc gcctgggcat agtcgggctt gcatttcagc
     4621 gccgcggcgt agcagtccag cgcctcttgc gccttggcca tgcgcttgta ggccacgccc
     4681 atattgttgt gggcctcggc atagttcggc tgcagcgccg cggcctgccg gaagctggca
     4741 atggcctcgt ccagctcgcc cagcgccatc agggccaggc ccaggttgtt gtggccgccg
     4801 gcatggccgg ggcgcttggc caggacttgc cgatagctcg cgacggactc gcggtattgc
     4861 ccgcgttcct gcaggtcgta cgcgtgctgg aatgccatct ctagcttggt tttcttcatg
     4921 gtgtcaggcg gcgtgccgct tgcggaaatc gtgcatgaaa cccaccaggg cctcggcggc
     4981 ctcgatggtc acgccgttgt agagcgaggc ccgcatgccg ccgatggcgc gatggccgtt
     5041 caagccggag aagcccgctt ctgacgacgc ctgcaggaac cgcctttcca gttcgggcgt
     5101 cgccatgttg aaggcgacgt tcatcgtcga tcgatggccc gcgtcggcgc ggcccttgta
     5161 atagccctcg ctatcgtcga tggcctggta cagcagcgcg gccttgcggc ggttgatcgc
     5221 gcccatctcg tccaggccgc ccacctcccg ggtcagccag cgcgtggcca gcatgacggc
     5281 gtagatggcg aagaccggcg gcgtgttgta gttggaatgc gccgtggcgt ggatgcggta
     5341 gtcgaggaac tcgggcaggt cggcaggcag cttgccgagc atctcgtcgc gcaccaccac
     5401 gactgtcacg ccggcggggc cgaggttctt ctgcgcgtgc gcgtagagga gggaatagcg
     5461 cgccgcctca cagggccggg acaggaagtc cgacgacatg tcgcacacgc gcggcacgtc
     5521 gtcgcggccc atgatctcct ggaactggat gccctcgacg gtctcgttgg agatgtagtg
     5581 gaaatacggg gcatcggccg agagcgccag gtccgccatg cgcggcaata tcctgaagcc
     5641 gtcgtccttg ccgctccagg ccacgttgat gcggtggcgc tgcgtggccg cggcaagcgc
     5701 cttgccgctc cagtagcccg tgagcaggta ctcgggcggc ggcgccggcg gacgcgccag
     5761 cgccatgggc agggtgaaga attgctgggt tgcgccgccc tgcaggaaca gcacatggaa
     5821 cccgtcgggc aggttcagca gcgcgcggac gttgcgctcg gcctcggcca ccaccgcggc
     5881 gaaccagtcc gagcggtggc tgatgcccag caccgacatg ccgacttccg gcacgcacag
     5941 catggactca tgcagttgct gcaggacgct ctccggcagg gcacccgggc cgccggagaa
     6001 gttgcgggca ttgcgcaatc gggctgtttc cagcatggtc aggctcctgt ttcgcgccct
     6061 gcctgggccg cgagcatgga ctggaatgcc gcgaaaacct tgtgcatctg cgccttcttg
     6121 tagggataaa cgtcttccgg gtccatgtcg tgcacgacgc cgcaggtatc ggcctcgaac
     6181 accaacagcc tgccgtccgg ggtctcgctg caatccatga ccaggtagtc caggccgacc
     6241 cgttcatgga agacgcgaaa ggccggcgca tggcgctcgc cgaagccctg gtcgaagcct
     6301 tgcatggcct cgccttcctc ggcgcggttg gccgcgttgt cttgcatgtc ggcgttgagg
     6361 tagtgcacca tccagcgcga cgagatgccc aggtgcgcca gataggcctt gccctgcatc
     6421 aggatgacgc gatacttgcg atagcgcccg tcggcgctgc ggtagtcgac gaactgcgac
     6481 acgaagaagt cattgcccgg cgtggcgccg aggtaggcgg ccagctccgc cggcgcgtcg
     6541 atcttgccca ggccttcgcc ggcgttggag tcgaccgggc gcaccaggat gggaaacacg
     6601 gcctcgggca gcacgtctcc caggcccgcc acgccgcgcg ccagttcgtc gagcgccagg
     6661 cggtcggtgc gcgccagcgc gggcgacacg
//
LOCUS       DASWPO010000116         7768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1812247, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000116 DASWPO010000000
VERSION     DASWPO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812247"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4218
                     /locus_tag="VGC69_06720"
     CDS             <1..4218
                     /locus_tag="VGC69_06720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0295012.1"
                     /translation="IDELVREGIAAGGSLPDETTVVYVSPLKALSNDIRANLEAPLAG
                     IADELRRLGLPDPGIRTAVRTGDTTQQDRQAMRKRPPHILVTTPESLYVLLTSASGRA
                     MLAKARTVIVDEIHAVAGSKRGSHLALSLARLDALCAQPPVRVGLSATQKPLDAVARF
                     LAGRGRGGDCAVVDIGHARPRDLALELPPVPLEAVMANDVWERVYDRMAELAGEHRTT
                     LVFVNTRRMAERAARHLADRLGAEVVAAHHGSLAKEHRLRAEQRLKHGELKVLVATAS
                     LELGIDIGDVDLVCQVGSPRGISALLQRVGRAGHHVGGVPRGRLFPTSRDDLAECVAL
                     LDCVRRGELDMLRIPRAPLDVLAQQIVAEVSCREWREDELYAMLRQAEPYAELPRERY
                     DAVMRMLTTGHLGRHGQRAVYLHRDTVSGTVRGRRGSQLAAMTSGGTIPDNADYTVLL
                     EPQSLNIGTVNEDFAVESLAGDVFQLGNASYRILRVEAGRVRVEDAHGMMPNIPFWLG
                     EAPGRSDELSAAVSRLRERVQALLDETQGDADRAASALAAELGIDAVSAGQLVGYLAR
                     ARAALGELPTQNTLVLERFFDESGGMQLVLHAPFGSRVNRAWGLALRKRFCRTFNFEL
                     QAAATEDAIILSLSTAHSFPLEEVWSYLRSASAEHVLVQALLDAPLFNVRWRWAAGVA
                     LALPRFAGGRKVAAPLQRMKSDDLLAAVFPDQAACLENIQGERELPSHPLVDQTLDDC
                     LHEAMDSEAWLAVLRRIEAGGCRLVERDLPAPSPLAMEILNANPYAFLDDAPLEERRT
                     QAVMARRWSDPAQAGDLGALDAEAIARVREEAWPQARDADEMHEALNDLACVTAAEAA
                     AQPGWAAWLRSLAAQGRACLLSEGGALGAEGERWVALERLGCLLAAYPGARAAPPLRP
                     RPEETEWEPEQARVEVLRARLAAGGPMTAAGLAGLLGWPPSAVAIALAALEREGAVLR
                     GRFSPGSVDDQWCERHLLARIHRYTIKRLRSEIEPVSRQDFMRFLFDWQHLAPQACLR
                     GPQALPKVLELLEGYEAAAGAWEDELLAARIADYEPAWLDELSRAGKIAWTRLAGAPR
                     AATAGRAGAGPVRATPLVILPRRQLRLWQDLAAGDAAAQPSARAQEVYQALLDGGAMF
                     YDELQAQTRLLPSQLEEALGELVAAGLATSDSYAGLRALLVPASRRGTRAGARRRRGA
                     MPDPLEAAGRWVPLRPAPVAADEPAGTGGARRQRSMWSANPPETLEHVARVLLRRYGV
                     VFWRLLDREPGWLPPWREWLPVLHRMEARGEVRGGRYVEGLAGEQFALPEAIALLREV
                     RHRPADGTLVRISAVDPLNVVGTLLPGEKVPAVVGNRILFRDGVPMAAWVAGKPRCDH
                     ELAAPERERLLRVLAGMPPFEEETAAA"
     gene            complement(4329..4820)
                     /locus_tag="VGC69_06725"
                     /pseudo
     CDS             complement(4329..4820)
                     /locus_tag="VGC69_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(4833..5723)
                     /locus_tag="VGC69_06730"
     CDS             complement(4833..5723)
                     /locus_tag="VGC69_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0295013.1"
                     /translation="MLKKIATDLLDIAYDEQGDTDGWPVVLLHGFPYDIHAYEDVAPR
                     LASQGARVIMPFLRGYGPTRFLAPATLRSGQQAALGADLLSLLDALGLEQAVLGGYDW
                     GGRAACVVAALYPSRARGLVSANGYNIQDIAAAMQPASPEKEHRLWYQYYLHGERGRA
                     GLTENRRAFCRLLWSLWSPTWRFDDATYARSAAAFDNPDFVDVVVHSYRHRFGLADGD
                     PQFDVMESRLSGMPSIAVPTITLEGDANGVAPPGRQADSGRFTGRHENRVVPNAGHNL
                     PQEAPEAFAAAVLDINAWAG"
     gene            complement(5746..6588)
                     /locus_tag="VGC69_06735"
                     /pseudo
     CDS             complement(5746..6588)
                     /locus_tag="VGC69_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            6688..7629
                     /locus_tag="VGC69_06740"
     CDS             6688..7629
                     /locus_tag="VGC69_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295014.1"
                     /translation="MKRILPPLNALRAFEAAGRLGSFKEAAAALHVTQGAVSQHVRLL
                     EAWLGAPLFERHNRRVALTAAAKAYLAEIGPLFEQLSQATARYGVPATVSRTLSVNAP
                     ATFTLRWLVPRLAKFRIEHVGVDVKVETSNEPVESLKEIHDVVIRGGPDTFYGYSMRP
                     FLSEERLPVCSPARLRRLPLRTPGDLRQHTLLHTTSLPRLWPDWLAKAQVCALRPAAT
                     LTFDHFYLTLQAAIDGMGIAMGPTALVSDDLAAGRLVAPFAGPRLPSRSYCTYVPDEK
                     SADELVVLFRSWLEREGACLLHAGTHRTAVAPRRRLR"
BASE COUNT         1178 a         2696 c         2744 g         1150 t
ORIGIN      
        1 atcgacgaac tggtgcgcga agggatcgcg gcgggcggca gcctgcccga cgagaccacg
       61 gtggtctatg tgtcgccgct aaaggcgctg tccaacgaca tccgcgccaa cctggaggcg
      121 ccgctggcgg gcatcgccga cgagctgcgg cggctcggcc tgcccgatcc gggcatccgc
      181 accgccgtgc gtaccggcga caccacgcag caggaccggc aggccatgcg caagcggccg
      241 ccccacatcc tggtgaccac gcccgaatcg ctgtatgtgc tgctcacctc ggccagcggc
      301 cgcgccatgc tcgccaaggc gcgcaccgtc atcgtcgacg agatccacgc ggtggccggc
      361 agcaagcgcg gcagccacct ggcgctgagc ctggcgcggc tggacgcgct gtgcgcgcag
      421 ccgccggtgc gcgtcggcct gtcggccacg cagaagccgc tggacgccgt ggcgcgcttc
      481 ctggcgggcc gcggccgcgg cggggattgc gcggtggtcg acatcggcca tgcgcgcccg
      541 cgcgacctgg cgctggaatt gccgccggtg ccgctggaag ccgtgatggc caacgacgtg
      601 tgggaacggg tgtacgaccg catggcggag ctggccgggg agcaccgcac cacgctggtg
      661 ttcgtcaaca cccggcgcat ggccgagcgc gcggcgcggc acctggcgga ccggctcggc
      721 gccgaggtgg tcgccgcgca ccacggcagc ctggccaagg agcaccggct gcgggccgaa
      781 cagcggctca agcacggcga gctgaaagtg ctggtggcca ccgcctcgct ggagctgggc
      841 atcgacatcg gcgatgtcga cctggtctgc caggtggggt cgccgcgcgg catttcggcg
      901 ctgctgcagc gggtcggccg cgccggccac cacgtaggcg gcgtgccgcg gggccggctg
      961 ttccccactt cccgcgacga cctggccgaa tgcgtggcgc tgctggactg cgtgcggcgc
     1021 ggcgagctgg acatgctgcg catcccgcgc gcgccgctgg acgtgctggc ccagcagatc
     1081 gtggccgagg tgtcgtgccg cgaatggcgc gaggacgaac tgtacgcgat gctgcggcag
     1141 gccgaaccct acgcggagct gccgcgcgag cgctacgacg cggtgatgcg catgctgacc
     1201 accggccacc tcgggcgcca cggccagcgc gccgtctacc tgcaccgcga cacggtgtcg
     1261 ggcacggtgc gcggccggcg cggcagccag ctcgccgcca tgacctcggg cggcaccatc
     1321 ccggacaacg ccgactacac cgtgctgctg gagccgcaga gcctgaacat cggcacggtc
     1381 aacgaggatt tcgcggtgga gagcctggcg ggcgacgtgt tccagctcgg caatgcctcg
     1441 taccgcatcc tgcgcgtgga ggcgggccgc gtgcgggtcg aggacgcgca tgggatgatg
     1501 cccaacattc ctttctggct gggcgaggcg cccggccgca gcgacgagct ctcggccgcc
     1561 gtgtcgcggc tgcgcgagcg cgtgcaggcg ctgttggacg agacgcaggg cgacgccgat
     1621 cgggccgcgt cggcgctggc cgccgaactg ggcatcgacg ccgtttccgc gggccagctc
     1681 gtcggctacc tggcccgcgc gcgcgccgcg ctgggcgagc tgcccacgca gaacacgctg
     1741 gtgctggagc gcttcttcga cgagtccggc ggcatgcagc tggtgctgca cgcgcccttc
     1801 ggcagccggg tcaaccgcgc ctggggcctg gcgctgcgca agcgcttctg ccgcaccttc
     1861 aatttcgagc tgcaggccgc cgccaccgag gacgccatca tcctgtcgct atcgaccgcg
     1921 cacagcttcc cgctggaaga ggtgtggtcg tacctgcgct cggccagcgc cgagcacgtg
     1981 ctggtccagg ccttgctgga tgcgccgctg ttcaacgtgc gctggcgctg ggccgcgggc
     2041 gtggcgttgg cgttgccgcg ctttgccggc ggacgcaaag tcgccgcgcc gctgcagcgc
     2101 atgaagagcg acgacctgct ggccgccgtc ttccccgacc aggccgcctg cctggagaac
     2161 atccagggcg agcgcgagct gcccagccac ccgctggtgg accagaccct ggacgactgc
     2221 ctgcacgagg ccatggacag cgaggcctgg ctggccgtcc tgcggcgcat cgaggccggc
     2281 ggctgccggc tggtggagcg cgacctgccg gcgccctccc cgctggccat ggagattctg
     2341 aacgcgaacc cctatgcctt cctcgacgac gcgccgctgg aagagcgccg cacccaggcc
     2401 gtgatggcgc gccgctggag cgatcccgcg caggccggcg acctgggcgc gctggacgcc
     2461 gaggccatcg cccgtgtgcg tgaagaggcc tggccgcagg cgcgcgacgc cgacgagatg
     2521 cacgaggcct tgaacgacct ggcctgcgtg acggccgccg aggccgctgc gcagccgggc
     2581 tgggcggcct ggctgcggtc gctggccgcg caggggcgcg cctgcctgct gtcggaaggc
     2641 ggcgcgctgg gtgccgaggg ggagcgctgg gtggcgctgg agcgtttggg ctgcctgctg
     2701 gcggcctacc ccggcgcccg cgcggcgccg ccgctgcggc cgcggcccga agagaccgag
     2761 tgggagccgg aacaggcgcg cgtggaagtc ctgcgcgcgc ggctggccgc gggcggcccc
     2821 atgacggcgg ccggcctggc cggcctgctg ggctggccgc catcggcggt ggcgattgca
     2881 ttggccgccc tggaacgcga aggcgccgtc ctgcgcggcc gcttcagccc aggctccgtg
     2941 gacgaccaat ggtgcgagcg gcacctgctg gcgcgcatcc accgctacac catcaaacgg
     3001 ctgcgcagcg agatcgagcc ggtgtcgcgc caggatttca tgcgcttcct gttcgattgg
     3061 cagcatctgg cgccccaggc ctgcctgcgc ggaccgcagg cgctgccgaa agtgctggag
     3121 ctgctggagg gctacgaggc cgccgccggc gcgtgggagg acgaactgct ggcggcgcgc
     3181 atcgccgact acgaaccggc ctggctggac gagctgtcgc gtgcgggcaa gatcgcctgg
     3241 acgcgcctgg ccggcgcgcc gcgcgccgcg actgccggcc gcgccggcgc ggggccggtg
     3301 cgcgccacgc ccctggtgat cctgccgcgg cgccaactgc gcctgtggca ggacctggcc
     3361 gcgggcgacg ccgccgcgca gccgtcggcg cgcgcgcagg aggtctacca ggccctgctg
     3421 gacggcggcg ccatgttcta cgacgaactg caagcccaga cgcggctgct gccgagccag
     3481 ctcgaagagg cgctgggcga actggtggca gccggtctgg cgacctcgga cagctacgcg
     3541 gggttgcgcg cgctactcgt tccggcctcg cgccgcggca cgcgtgccgg cgcgcgccgg
     3601 cggcgcggcg cgatgcccga cccgctggaa gcggccggac gctgggtgcc gctgcggccg
     3661 gcgccggtag ccgcggacga gccggccggc accggcggcg cgcggcgcca gcgctcgatg
     3721 tggtcggcca acccgcccga gaccctggag catgtcgcac gcgtgctgct gcggcgctat
     3781 ggcgtggtgt tctggcgcct gctcgaccgc gagcccggct ggctgccgcc ttggcgcgaa
     3841 tggctgccgg tgctgcaccg catggaggcg cgcggagagg tgcgcggcgg ccgctatgtg
     3901 gagggcctgg cgggcgagca gttcgccctg ccggaagcca ttgcgctgct gcgcgaagtc
     3961 cggcaccggc cggcggatgg cacgctggtc aggatctcgg cagtcgatcc gctgaacgtc
     4021 gttggcacgc tgctgcccgg cgagaaggtg ccggcggtgg ttggcaaccg catcctgttc
     4081 cgcgacggcg tgcccatggc cgcctgggtg gccggcaagc cgcgctgcga tcacgaactg
     4141 gcggcgcccg agcgcgaacg cctgttgcgg gtgctggccg gcatgccgcc gtttgaggaa
     4201 gaaaccgcgg cggcttgaga acccgccgag tgtttggcaa cgaacgacac agcctacggc
     4261 acgtgcagga tatccgcatc aggcggatgc cggccgcgcc atggcgtcca ctctcgtgcg
     4321 cgccgtgctc acttgccgcc ggtgatgtcg aggaacgtgc ccgtgacgaa ggaagcctcg
     4381 gcgctcgcca gccataggat cgcccgcgca acctcttcgg gttgcccccc tcttcccata
     4441 gggatcgagt ctttgacgcg atccaccctc cccggctctc cgccgctggc atgcatgtcg
     4501 gtgtagatgt gcccagggcg tatgcagttg acgcgtatcc cctcccgcgc gacttccttg
     4561 gcgaagccgg tggtgaatgt ctcgacggcg cccttggaca cggcgttgtt caccagcacg
     4621 tcgatccggc cgaattgccg gtcgatcgcg gcgaacagcc gagcgacctg ttcaggatcc
     4681 gcgctgtctg cgcgcacggg taaagcccgg cgcccaaggg ctttgacgtc ggccgccacg
     4741 gcgagggcgg cggactcgtt ggagacgaag ctgatcgcga catcgtaacc ttgcgcggcg
     4801 gcaagccgcg cagtcgccgc gcgcgggcag catcaaccgg cccaggcgtt gatatcgaga
     4861 accgcggcgg cgaacgcttc cggcgcctcc tgcggaaggt tgtgccccgc attgggcacg
     4921 acgcggtttt catggcgtcc cgtgaagcgc cctgagtcgg cttgtcttcc cggcggcgcg
     4981 acaccgttcg cgtccccctc gagggtgatc gtgggcacgg cgatcgacgg catgccggac
     5041 aagcggctct ccataacgtc gaactgggga tcgccgtcgg ccagtccgaa acggtgccga
     5101 tacgaatgga ccacaacgtc gacgaaatcc ggattgtcga acgccgctgc cgagcgcgcg
     5161 taggtggcgt cgtcgaagcg ccaggtcggc gaccacaagg accacaagag ccggcagaac
     5221 gcgcgccggt tttcagtcag cccggcgcgg ccgcgctcgc catggagata gtattggtac
     5281 cagagccggt gttctttttc ggggctggct ggctgcatgg cggcggctat atcctggatg
     5341 ttgtagccgt ttgccgaaac cagtccacgc gctctcgacg ggtacagcgc ggccacgacg
     5401 caggctgccc ggccgcccca gtcatacccg cccagcacgg cctgttcaag cccaagcgca
     5461 tcgagcagcg atagcaggtc tgcccccagc gccgcctgct ggcccgaacg cagcgttgcc
     5521 ggcgcaagaa aacgcgttgg cccatagccc cgcagaaaag gcatgatgac gcgggcgccc
     5581 tgggatgcca ggcgcggcgc cacgtcctcg tacgcgtgga tatcgtaggg aaagccgtgc
     5641 agcaggacga ctggccatcc gtcggtatcg ccctgttcgt cataagcgat gtcaagcagg
     5701 tcggtggcaa tttttttaag catgaatcca tcctttcagg caatgtcaaa gccccaggca
     5761 aaccgccccg cccgcgacga tcacgcacgc gagccagcgt tttccactga ccgtttcccg
     5821 caggaacatt catccgatca gcaccgcaaa cacgacacta gtctcgcgca aggccgacac
     5881 catgcccatc gcgcccgact gcaacgcccc tatcacgatg ccatacgccg cgatcgacac
     5941 caacccgccg atcagcgatg agccgatcac cctgggcgcc gcccggaccg gcgcccacag
     6001 cgccgcgagc ccgcgcgtcg cgacgaacag caccggcatc agccaataga acaggaacat
     6061 ccaggcggtg taggcgaccg cctggccgcc ggacaagcgc acgccgatgc cgtcgatcac
     6121 ggtatagaac gcgatcgtcg cacccgtcgt cagcgcggcc agtgcgcccg cgcgcgacac
     6181 atggcgccct tccagtgcaa tggcaatgat gccacccgat atcatcgcga tccccaaagc
     6241 gtgccaggga ccgatcgcct catgcgcgaa gagcgccgcg ccgagcgcga cgagcagcgg
     6301 cgatgagccg cgcgcgatcg gatacgcctg cgccagatcg ttgcggcggt acgaccgcac
     6361 gagggtcacg ttgtaggcga tatgcacgag ccccgatgcg gcgatgcacg gccaggccgc
     6421 gccggccggc cacggtgcga acaggatcgc gaccgtggag accgccgcga tggcgatgct
     6481 catccacgtc atggacagca agcggtcgtc gctgccgtgc agcatcgcgt tccagctcgc
     6541 gtggagcagt gccgcaagga ggacgaaacc gccggtatga ctgagcatgc gggtccagga
     6601 caaaaggtct ggcgagaagg atatctatct tgtgatgggc taacaaacca gataaattgc
     6661 ccattcgcat tagataaact atttcgaatg aagcggatat tgcctccact caatgcattg
     6721 cgtgccttcg aggccgcggg ccggctaggc agcttcaagg aagccgccgc ggcattgcac
     6781 gtgactcagg gtgcggtgag ccagcacgtg cgcctgcttg aggcgtggct gggcgccccg
     6841 ttgtttgaac ggcacaaccg gcgtgtggcg ctcactgccg cggccaaggc ctacctggcg
     6901 gaaatcggcc ccctgttcga gcagctctcg caggcaaccg ccagatatgg cgtcccggcg
     6961 acggtctccc ggaccctgtc cgtcaatgcg cccgcgacgt tcaccttgcg ctggctggtg
     7021 ccgagactgg cgaaattccg catcgagcat gtcggcgtgg acgtcaaggt ggagacgtcg
     7081 aacgagccgg tggagagtct gaaagagatc catgacgtcg tcatccgggg cggcccggac
     7141 acgttctacg gctattcgat gcgccctttc ctgtccgagg agagactccc ggtctgcagt
     7201 ccggcacgct tgcggcgact gccgcttcgg acgccaggcg atctgcggca gcatacgttg
     7261 ctgcacacga cgagtctgcc gcggctctgg ccggactggc tggcgaaggc gcaggtttgc
     7321 gcactccggc cggcggccac cctgaccttc gatcacttct atctgacgtt gcaggctgcc
     7381 atcgacggca tgggcatcgc gatgggaccg actgcgctgg tatcggacga ccttgccgcc
     7441 ggccggcttg tcgcgccgtt cgctggccct cgcctgccgt cgcggagcta ttgcacttac
     7501 gttccggatg agaagtccgc ggatgagctg gtcgtgctgt tccgctcatg gctcgagcgc
     7561 gagggggcat gcctgcttca tgccggtacg catcgaactg ctgtcgcccc acgccggcgc
     7621 ctgcgctgaa gatcgatttt gcgcagcccg cactcgggtt tcccataggg gaatcgtgct
     7681 tgcctatact tgtatattca agctagagtt tgcgccatgt tccaacctat ccgccacctt
     7741 gggcctatag gacgccatgc ccagcgcc
//
LOCUS       DASWPO010000117         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1097163, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000117 DASWPO010000000
VERSION     DASWPO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097163"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGC69_06745"
     CDS             complement(<1..281)
                     /locus_tag="VGC69_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0295015.1"
                     /translation="MMKREKYELLALAAVWGGSFICLRIAAPEFGPSALMFLRVGLAV
                     LVVLPVTVMRGGDLMLAARRWRPLLLVGAFNMAFPFLAYAYAGKVLGAG"
     gene            complement(300..1649)
                     /locus_tag="VGC69_06750"
     CDS             complement(300..1649)
                     /locus_tag="VGC69_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295016.1"
                     /translation="MLGVAATVVLVAFDSTIVSTTLPRVAEALNGLALYSWVGTGYLL
                     TTAASILIFGRMGDMFGRKPMMMVSVSLVALGSISCGLSQTMLQLTLFRALQGIGGGM
                     MIATAFAAPADLFPEARERVRWLALTTSSYAMASGIGPVLGGAATQALGWRAAFFIAP
                     VTAVIGLVLIGRYFPTIPPHGSGPRRIDWLGCILLVVLVASPLAALQLGFATSGVSHP
                     LVALCLALAAIVAALALPPHLRRKEMPIFPLRVLASKEARMLNMAAIATGAVMFILIF
                     YSPLLLQREFGYTPSQAGLLITPQVVAIAIGSLINGRLFPRQAEPQRLLAFGGWLLAA
                     GSLMVLAIGDGTPVAWILLSFFTTGAALGFVLANLTLFIQMLSERRDVGTASALVQTT
                     RAIGSALGTALVGIAVSHTSVRVGVRIGLVLCVAMALTTAFVGHKITMRNVMQKRSA"
     gene            complement(1871..>2590)
                     /locus_tag="VGC69_06755"
     CDS             complement(1871..>2590)
                     /locus_tag="VGC69_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEY0295017.1"
                     /translation="EFFLRKGYPTYVVDQASRGRSAADPTTTVGARQGKLPPGQLPAV
                     FSAGREGAWKIFRFGPEYPKIYAGLQYPLQAQDQLWKQMVPDWSAAQAVPNPTVPDLS
                     LLVKRLGGAILVSHSQSGIYPFQVASIDPSGIAAIVALEPGSCPKPDEDVRPYTHIPT
                     LLVYGDFIAQSTDWWVPRLKACEAFVEAINAAGGKARIVHLPDVGFHGNTHMMMQDLN
                     SLDVAEWLSRWLDGHGGQQRG"
BASE COUNT          465 a          888 c          883 g          354 t
ORIGIN      
        1 aagcccgcgc ctagcacctt gcccgcgtag gcgtaggcca ggaaggggaa ggccatgttg
       61 aaggcgccca ccagcagcaa ggggcgccag cgcctggcgg ccagcatcag gtcgccgccg
      121 cgcatgacgg tcacgggcag caccaccagg actgccagcc ccacgcgcag gaacatcagg
      181 gccgaggggc cgaactccgg cgcggcgatg cgcaggcaga tgaaagaccc gccccacacc
      241 gcggccaggg ccagcagttc gtatttttcc cgcttcatca tgacggcttc tgcgacgact
      301 caggcgctgc gtttttgcat gacgttgcgc attgttatct tgtgtcccac gaaggccgtg
      361 gtcaaggcca tggccacgca caataccagg ccgatgcgca cgcccacgcg caccgaggtg
      421 tgcgagacgg cgatgcccac cagcgccgtg cccagcgcgc tgccgatggc ccgcgtggtc
      481 tgcaccagcg ccgacgccgt gcccacgtcg cggcgttcgc tcagcatctg gatgaagagg
      541 gtcaggttgg ccagcacgaa acccagcgcc gcgcccgttg tgaaaaacga cagcaggatc
      601 cacgccaccg gcgtgccgtc gccgatggcc agcaccatca gcgagcccgc cgccagcagc
      661 cagccgccaa acgccaggag gcgctgcggc tcggcctgcc tggggaacag gcggccgttg
      721 atcaggctgc cgatggcgat ggccaccact tgcggcgtga tcagcaggcc ggcctggctg
      781 ggcgtgtagc cgaactcgcg ctgcagcagc agcgggctgt agaagatcag gatgaacatc
      841 accgcgccgg tggcgatggc cgccatgttc agcatgcgcg cttccttgct ggccagcacg
      901 cgcaacggaa agatcggcat ttccttcctg cgcaggtgcg ggggcagcgc gagcgccgcc
      961 acgatcgccg caagggccag gcacagcgcg accagcggat gcgagacgcc gctggtggca
     1021 aagcccagtt gcagggcggc cagcggcgag gccaccagca ccaccagcaa aatgcagccc
     1081 agccagtcga tgcgccgcgg cccgctgccg tgcggcggga tggtcgggaa atagcggccg
     1141 atgagcacca gcccgatcac ggcggtgacc ggcgcgatga aaaacgccgc gcgccagccc
     1201 agcgcctggg tcgccgcgcc gcccagcacc ggcccgatgc cgctggccat ggcatacgac
     1261 gaggtggtca gcgccagcca gcgcacccgc tccctggctt cggggaacag gtccgccggc
     1321 gccgcgaagg ccgtggcgat catcatgccg ccgcctatgc cctgcagggc gcggaagagg
     1381 gtcagctgca gcatggtctg cgacaggccg caggaaatcg accccagcgc caccagcgac
     1441 accgacacca tcatcatggg cttgcggccg aacatgtcgc ccatccggcc gaagatcagg
     1501 atgctggccg ccgtggtcag cagatagccc gtgcccaccc aggaatacag cgccagcccg
     1561 ttcaaggcct cggccacgcg cggcagcgtg gtgctgacga tggtggaatc gaacgccacc
     1621 agcaccaccg tggcggccac gcccagcatc gccagcaaga gctcgcggcg ggatcgggcg
     1681 ccaggggcgg cgggtgccgg gatggattcg tctggcgtgg gcggggcgtt catgacgccg
     1741 taagaataca ccggcgctcc tgtcaggaac acgacgggag gcgcccaacc gcgccggcac
     1801 tggcatgctg gggtttattt cgccgccgct atgcccagtt gcccgtgccc gcatcgacgg
     1861 ctcgcggcca tcagccgcgc tgctgtccgc cgtgcccgtc cagccagcgc gacagccact
     1921 cggcgacgtc caggctgttg aggtcctgca tcatcatgtg cgtattgccg tggaaaccca
     1981 cgtccggcag gtgcacgatg cgcgccttgc cgcccgccgc attgatggcc tctacgaacg
     2041 cctcgcacgc tttcagcctg ggcacccacc aatcggtgga ttgtgcgatg aaatcgccgt
     2101 agaccagcaa ggtagggata tgcgtgtacg gacggacgtc ttcgtcgggc ttggggcagg
     2161 agcctggctc gagggcgacg atggcggcga tgccggaggg atcgatcgag gcgacctgga
     2221 agggatagat gccagactgg gaatggctca ccaggattgc gccgcccagg cgctttacca
     2281 atagcgacag gtcgggaacg gtcgggttgg gcacggcctg cgccgcgctc cagtcgggca
     2341 ccatctgctt ccagagctgg tcttgcgcct gcaaggggta ctgcaggccg gcgtagatct
     2401 tgggatactc ggggccgaaa cggaaaatct tccacgcccc ctcgcgtccc gcggaaaaca
     2461 ccgccggcag ctgcccaggc ggcagcttgc cctgcctggc gccgaccgtt gtcgtgggat
     2521 cggccgcgga ccggccgcgc gaggcctggt ccaccacata ggtgggatag cccttgcgca
     2581 ggaagaattc
//
LOCUS       DASWPO010000118         7226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1454013, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000118 DASWPO010000000
VERSION     DASWPO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454013"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VGC69_06760"
     CDS             complement(<1..397)
                     /locus_tag="VGC69_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0295018.1"
                     /translation="MSTIQSVSVCIARVPLDSPVTFATRQVTAREYCLVRVRDTEGRE
                     GLGYCYAVNSAGRLLSVAVTDLLAPKLVGQDSLRVEGLWTEMYQEALLMGRAGGVMRS
                     LSALDIALWDLNARAAGLPLYRYLGASAHD"
     gene            complement(1048..1794)
                     /locus_tag="VGC69_06765"
     CDS             complement(1048..1794)
                     /locus_tag="VGC69_06765"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY0295019.1"
                     /translation="MIELGVNIDHVATLRQQRHTIYPDPIEAAVRAEEAGADLITLHL
                     REDRRHIQDADVRAIRGRLRTRMNLECAVTREMLDIACEVGPDDVCLVPEKRAELTTE
                     GGLDVIGGQAAVAAAVGQLGESGIRVSLFIDPEPSQIDAAAQAGAPVIELHTGAYAEA
                     QTPQAAQAELERVRVAVAHGLRLGLRVNAGHGLHYGNVAPVAALAGISELNIGHAIVA
                     RAVFDGWEKAVRDMKALMVQARLQAQRGRA"
     gene            complement(1787..2548)
                     /locus_tag="VGC69_06770"
     CDS             complement(1787..2548)
                     /locus_tag="VGC69_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY0295020.1"
                     /translation="MRRPFRILHARRALIAVLALASVLPAAQAARRLPAHPPPAAQAP
                     APAPVPQAAESAVTAPLPEQAPLDRQIDMIRRILPQAKRVGIIYNPGQPTAAAAVKQL
                     LDLLPKAGMNPVEITAPRQVDVGPAVRNLIGRVDLVYTIFDDNVTAEYDIVARTCDEA
                     HIPLFAADPANVRRGAIASLGLAGKEAAPAVKAAAARPVRPERPVAGPRLDLYINPDA
                     AARQGVALSDAVLKSAVLVSQANSSPRGADGVKHD"
     gene            complement(2559..3329)
                     /locus_tag="VGC69_06775"
     CDS             complement(2559..3329)
                     /locus_tag="VGC69_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY0295021.1"
                     /translation="MSSRRTPSEHDQPTLSEMDGIRYLHFNTEWVQGAMRIADPSALV
                     LNYTMQMMAWLLFLAPPREAPIGLLGLGAGSLARFCLKRTRSDLEIVEWNPRVTAACQ
                     TFFRLPPSPRMKIHHLDAGLWVADPLNVGKCPVLMVDLYDAQALGPVRDSVAFYRACR
                     RVLGEVGVLVVNLFGRHESFGRNIDNLSAAFDDRVILLPEIDEGNQIVMAFSGPSLAV
                     TPAQLLERAEQVEAEYGLPARRWARALLRHAVDGVLYL"
     gene            complement(3378..4658)
                     /locus_tag="VGC69_06780"
     CDS             complement(3378..4658)
                     /locus_tag="VGC69_06780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0295022.1"
                     /translation="MNPLLSAVDRKLKSVGDVPIQLVMPDGTALGSSDPRVKFVVRNK
                     KALVHLAEGSVGVLGQDYVEGLVDIQGTMRDVMAAASRLLSDSPIDEGASSWATGFVR
                     KIISIWRHSIERDAKQIEFHYDLSEEFYALWQDPHRVYSCAYYRTPEMNLAQAQEAKL
                     DHICRKLRLAPGERFLDIGAGWGGLLLWAAENYGVDATGITLSRNQHAHVNRLIQEKG
                     LSGRVRMELLDYRKLDESQPFDKISSVGMFEHVGRALLPEYFAKLRRLLKPGGLIMNH
                     GITSGGVYNAEVGSGMGQFIDRYIFPGGELTHASNVLQAVALGGLETVDMENLRPHYA
                     RTLWAWSDALESQLDRAAEILTKDKGEQGMKSLRAYRLYLAGCAMGFEHGWISLHQFL
                     LAPLATGRSDELDYPADLSFPWRRDYMYDGKQKA"
     gene            4868..6010
                     /locus_tag="VGC69_06785"
     CDS             4868..6010
                     /locus_tag="VGC69_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY0295023.1"
                     /translation="MGVLTAIAPISIDMYLPAFPAIAAGLGVPRGEVERTLAVYLGGL
                     ALAQLVYGPLADRFGRKLPVLCGLALYCVAVLGCALAPNIQILMLCRLLQALGGSVGL
                     VVPRAVIRDHYNTQDAARALSLVMLVMGLAPILAPIIGGQVLLLTSWRGMFWLMEAYG
                     LALLAATALAMRESLLPERRIPLRPSLIASNYLGLLSHRRFMAHSLAGGMGQAGMFTY
                     ILGSPRVFIEIYGVPTQYYGLLFGLNAAALICGAQVSARLLRRHTSLALQGRALVTLA
                     LAGLLALALTLAGVMNLWLLMLCLLGFMASQGFVNPNSVALALAEQGHRLGASSALLG
                     TLQFLCGALAGLAVSLWQTQSPLPLVTVMACCACLGWLSGRVARLD"
     gene            6318..6554
                     /gene="rpmB"
                     /locus_tag="VGC69_06790"
     CDS             6318..6554
                     /gene="rpmB"
                     /locus_tag="VGC69_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY0295024.1"
                     /translation="MARVCQVTGKGPMVGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWIRLRVTTKAIRTIDKNGIDAVLADLRARGEAV"
     gene            6609..6779
                     /gene="rpmG"
                     /locus_tag="VGC69_06795"
     CDS             6609..6779
                     /gene="rpmG"
                     /locus_tag="VGC69_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY0295025.1"
                     /translation="MAAKGNREKIKLESTAGTGHFYTTTKNKRNTPDKMLLKKYDPVA
                     RKHVDYKETKLK"
     gene            6898..>7226
                     /locus_tag="VGC69_06800"
     CDS             6898..>7226
                     /locus_tag="VGC69_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295026.1"
                     /translation="MLRHLPTRLAACLLAALCAAPISASGRPAAQRHAARPAEPPPIR
                     IGEINSYKARPELLEPYRKGWQMALDEINQQGGVLGRKLQVIVRDDGGTAEGALAAAR
                     QLAEGEQV"
BASE COUNT         1199 a         2449 c         2311 g         1267 t
ORIGIN      
        1 ggtcatgcgc cgacgcgccc aggtagcggt acagcggcag cccggcggcc cgggcattca
       61 ggtcccacaa cgcgatgtcc agcgccgaca gcgaacgcat gacgccgccc gcacgcccca
      121 tcagcagcgc ttcctggtac atctccgtcc acaggccctc cacgcgcagc gaatcctggc
      181 ccaccagctt gggcgccagc aggtcggtca cagcgaccga tagcagccgg ccagcgctgt
      241 tcactgcgta gcagtagccc agcccctccc ttccctctgt atcgcgcacg cgcaccaggc
      301 aatactcgcg cgctgtgacc tggcgcgtgg cgaacgtgac cgggctgtcc agcggaaccc
      361 gggcaataca tacggaaact gactgaatgg tagacattgg attaccggct ccttgggaga
      421 tgtgttgttg aatgattcga cctcaatact tgttcttgtc gctgtggacg aagccaggta
      481 tctcgtcgct gaggcagtcg gtgatagctc tgaccttgag cgtatgttcc tagacgaagg
      541 acacgctctg agtgggtcgg gcgaagctct gtgaaaaccg gtggtcttgc actgcgcttt
      601 gacgacttgg ccggcggtag cacaggtcag gtcgacgatt ccccgcatcg ggcgagacgg
      661 gaattccata cgatgtctct tccaaggatt ctctgcgtgt gtttatggtt tatggcggga
      721 atcttatgcc gttctctaag ccaataaatg gattttttat ctcaactaat agatttttta
      781 acttaattgg aaaaacatcc gaggcatacg ggattgccgc tagacaacgc tgagaccggg
      841 cgttgtctgc tgattacata aggctctcgg ttgatcgggc gctacaacag ctagctggcg
      901 atggcgaaca aggacatgat gctgtagctg tatcagccgc tgttctccat cgaagggggc
      961 aagatggaca taacgagcaa gctacggctc tcgaccccta cgcggcgcca cacgagcaga
     1021 actatggcga ggaacgagga ttgaggttca ggcgcggccg cgctgcgcct gcaggcgggc
     1081 ctggaccatc agcgccttca tgtcgcgcac ggccttttcc cagccgtcga acacggcccg
     1141 cgccacgatg gcatggccga tattgagctc cgagataccg gccagcgccg ccaccggcgc
     1201 cacattgccg tagtgcaggc catgcccggc gttcacgcgc aggcccaggc gcaggccatg
     1261 ggccacggcc acgcgcaccc gctccaactc ggcttgcgcc gcctgcggcg tctgcgcctc
     1321 ggcataggcg cccgtatgca gttcgatgac cggggcgccg gcctgcgccg ccgcatcgat
     1381 ctggctgggc tcggggtcga tgaacagcga cacgcggatg ccggactcgc ccagctgccc
     1441 gacggccgcc gccacggcgg cctgcccgcc gatcacgtcc aggccgccct cggtcgtgag
     1501 ctcggcgcgc ttctccggca ccaggcagac gtcatcgggc ccgacctcgc aggcaatgtc
     1561 cagcatctcc ctggtcacgg cgcactccag gttcatgcgg gtgcgcagcc tgccgcggat
     1621 cgcgcgcaca tcggcatcct ggatatggcg ccggtcttcg cgtagatgca gggtgatgag
     1681 gtcggcgccg gcctcttcgg cgcgcaccgc cgcctcgatg ggatcgggat aaatcgtatg
     1741 gcgctgctgc cgcagcgtgg ccacgtggtc gatattgacg cccagttcaa tcatgtttga
     1801 ctccatccgc accgcgcgga ctgctatttg cctggctgac cagcaccgcc gacttgagga
     1861 ccgcatcgga cagcgcgact ccctgtcgcg cggcggcgtc cggattgatg tagagatcga
     1921 gccgcggccc cgcgaccgga cgctcgggcc gcacaggccg cgcggcagcg gccttgaccg
     1981 cgggcgccgc ttccttgccc gccagcccca gcgaggcgat ggcgccgcgc cggacattgg
     2041 ccggatcggc ggcgaacagg ggaatatgcg cttcgtcgca ggtcctggcc acgatgtcgt
     2101 attcggccgt gacgttgtcg tcgaagatgg tgtagacgag gtcgacgcgg ccgatcaggt
     2161 tgcgcaccgc cggccccacg tccacctggc gcggcgccgt gatctccacc gggttcatgc
     2221 ctgccttggg caacaggtcg agcagttgct tgacggcggc ggcggcggtc ggctggcccg
     2281 ggttgtagat gatgcccacg cgcttggcct ggggcagtat ccgccggatc atgtcgatct
     2341 gcctgtccag cggcgcctgc tcgggcaagg gcgccgtcac ggcggattcc gcggcctggg
     2401 gaacgggagc gggagccggc gcctgggcgg cgggcggcgg gtgcgccggc agccgccgcg
     2461 cggcctgcgc cgcgggcagg accgaagcca gcgccagcac cgcgatcaag gcgcggcggg
     2521 catgcaggat ccggaacggg cgacgcatgt cggcttcctc agaggtaaag cacgccatcg
     2581 acggcatggc gcagcagggc ccgcgcccag cgccgcgccg gcaggccgta ttcggcctcc
     2641 acctgttccg cgcgctcgag cagttgcgcc ggcgtcacgg ccagggacgg gccgctgaac
     2701 gccatcacga tctggttgcc ctcgtcgatc tcgggcagca ggatcacgcg gtcgtcgaat
     2761 gcggcggaaa gattatcgat attgcggccg aagctttcat gccggccgaa caggttcacg
     2821 accagcacgc ccacttcgcc cagcacccgc cggcatgccc ggtagaaagc caccgagtcg
     2881 cgcaccggcc cgagcgcctg cgcgtcgtac aaatccacca tgagcaccgg gcacttgccc
     2941 acattgagcg gatccgccac ccacaggccc gcatccaggt gatggatctt catccggggc
     3001 gagggcggca ggcgaaagaa agtctggcag gccgccgtga cccggggatt ccattcgacg
     3061 atttcgaggt cgctgcgcgt gcgcttgagg caaaagcgcg ccagcgaacc cgcccccagc
     3121 cccagcaggc cgatgggcgc ctcgcgcggc ggcgccagga acagcagcca cgccatcatc
     3181 tgcatcgtgt agttcagcac cagggcggag ggatcggcga tgcgcatcgc gccctgcacc
     3241 cattcggtat tgaaatgcag atagcggatg ccgtccattt cggacagcgt gggctggtcg
     3301 tgctcggagg gcgtgcggcg gctggacatc ggcgaattgt agccagcgtc cggccacgcg
     3361 cgcatcgacc cggtggatca ggccttctgc ttgccgtcgt acatgtagtc gcggcgccag
     3421 gggaaggaca ggtcggccgg gtagtcgagc tcgtcggaac ggcccgtggc cagcggcgcc
     3481 agcaggaact ggtgcagcga aatccagccg tgctcgaagc ccatggcgca gccggccagg
     3541 tacaggcggt aggcgcgcag cgacttcatg ccctgctcgc ccttgtcctt ggtgaggatc
     3601 tccgcggcgc gatcgagctg tgactccagt gcgtcgctcc aggcccacag cgtgcgggcg
     3661 tagtgcggac gcaggttttc catgtcgacc gtttccagcc cgcccagggc cacggcctgc
     3721 agcacgttgc tcgcgtgcgt gagctcgccg cccgggaaga tgtaccggtc gatgaactgg
     3781 cccatgccgc tgcccacctc ggcgttgtag acgccgcccg aggtgatgcc gtggttcatg
     3841 atgaggccgc cgggcttgag caggcggcgc agcttggcga agtattcggg cagcagggcg
     3901 cggccgacgt gctcgaacat gcccaccgag gaaatcttgt cgaagggctg cgactcgtcg
     3961 agcttgcggt agtcgagcag ctccatgcgc acccggcccg acaggccttt ttcctggatc
     4021 aggcggttga cgtgcgcgtg ctggttgcgc gagagcgtga tgcccgtggc atccacgccg
     4081 tagttctcgg ccgcccacag cagcaggccg ccccagccgg cgccgatgtc caggaagcgc
     4141 tcgcccggcg caaggcgcag cttgcggcag atgtggtcga gcttggcctc ctgggcctgc
     4201 gccaggttca tttcaggcgt gcgatagtag gcgcacgaat agacccggtg cggatcctgc
     4261 cacagggcgt agaactcttc ggacaggtcg tagtggaact cgatctgctt ggcatcgcgc
     4321 tcgatggagt ggcgccagat cgagatgatc ttgcgcacga agccggtcgc ccaactgctg
     4381 gcgccttcat cgatgggaga atccgaaagc aggcgcgaag cggccgccat gacatcgcgc
     4441 atggtgccct ggatgtcgac caggccttcg acgtaatcct ggcccagtac gccgacggag
     4501 ccttccgcca agtggaccag cgccttcttg ttgcgcacga cgaacttcac gcgcggatcg
     4561 gacgagccca gggcagtacc atcgggcatc accaactgaa tcggcacatc ccccacactc
     4621 ttcagcttcc ggtccactgc ggacaacaac gggttcacga gcctttctcc ccagataatt
     4681 tgaatgaact agccggactt tatcggaaaa tagcttaaat ttgtttgcaa gctttaccgt
     4741 gatacatttg caggtcggcg tcgtgatttt gcctcgattc ctctgcctgg cgccacaccc
     4801 ccccttttcc tcccccatgt cgtcttccga aacgatgcct cgcaagctgc ctggttggct
     4861 gcttttgatg ggcgttctca ccgcgatcgc cccgatttcc atcgatatgt acctgcccgc
     4921 cttcccggct atcgccgcgg ggctgggggt gccgcgcggc gaggtcgagc gcaccctggc
     4981 ggtctacctg ggcggcctgg cgctggcgca gctcgtctac ggcccgctgg ccgaccgttt
     5041 cggccgtaaa ttacccgttc tgtgcggatt ggcgctctac tgcgtcgccg tcctgggctg
     5101 cgcgctcgcc cccaatatcc agatcctgat gctgtgccgc ctgctgcagg cgctgggcgg
     5161 atcggtcgga ctggtggtgc cgcgcgccgt catccgcgat cactacaaca cgcaggacgc
     5221 ggcccgggcc ttgtccctgg tcatgctggt gatgggtctg gcccccatcc tcgctccgat
     5281 catcggcggc caggtcctgc tgctgaccag ctggcgcggc atgttctggc tgatggaggc
     5341 ctacggcctg gcgctgctgg ccgccacggc cctcgccatg cgcgaatcgc tgctgcccga
     5401 gcgccgcatc cccttgcgcc ccagcctcat cgcctcgaat tatctcggcc tgctgagcca
     5461 ccggcgcttc atggcccatt cgctggccgg cggcatgggc caggccggca tgttcaccta
     5521 catcctgggc tcgccgcggg tgttcatcga aatctacggc gttcccacgc agtattacgg
     5581 cctgttgttc ggcctgaacg ccgccgcgct gatctgcggc gcccaggtca gcgcccgcct
     5641 gctgcgccgg catacgtcgc tggccctgca aggccgcgcc ctggtcacgc tggccctggc
     5701 gggcttgctg gccctggcgc tgaccctggc gggcgtcatg aatctctggc tgctgatgct
     5761 ctgcctgctg ggcttcatgg ccagccaggg cttcgtcaac ccgaactcgg tggccctggc
     5821 gctggccgag cagggccacc gcctgggcgc ctcctcggcc ctgctgggca ccctgcagtt
     5881 cctgtgcggc gccctggccg gcctggccgt cagcctgtgg cagacccaga gccccctgcc
     5941 cctggtgacg gtgatggcct gctgcgcctg cctgggctgg ctatccggcc gggtggcccg
     6001 cttggactga aacgtccaag cacttgactt gccttgattt ttcgtattag aatttcagat
     6061 tcttagcagt ttcccgtttc gcacccgcta tgctttgcgg gcgcgtaatc cgaattaagg
     6121 cttcgcagct ttttgtggcg ggtcgaaccg gcggggtata agcacgcaag acgccatctg
     6181 ccttgtgcct gtcgagacga cgcggcacct gcgcccgagg cggcccgaac ccgggaatcc
     6241 ggttcggccc agcgcgcttc tcccccttcc cgtctcgcca caagcctcga agcaaaacaa
     6301 caaccgagag gtgcatcatg gcacgtgttt gccaagttac cggcaagggc ccgatggtgg
     6361 gcaacaacgt ttcgcacgcc aacaacaaga ccaagcgtcg cttccttccc aacctgcaat
     6421 cgcgccggtt ctgggtcgaa agcgaaaacc gctggattcg actgcgcgtg acgaccaagg
     6481 ccatccgcac gatcgacaag aacggcatcg acgctgtgct cgccgacctg cgcgcccgcg
     6541 gcgaagcggt ctgagacatc cggtcttcga tcccgccagc ctaatcccac acattccagg
     6601 agcacgacat ggccgccaaa ggcaaccgtg aaaagatcaa gctcgagtcc accgcgggca
     6661 cgggccattt ctacaccacc accaagaaca agcgcaacac gcccgacaag atgctgctca
     6721 agaagtacga tcccgtcgcg cgcaagcacg tcgactacaa ggaaaccaag ctgaagtaat
     6781 tcggcggttt cggatctacg gcatcgtagt atcgttgcga ggccctgctc atgcagggcc
     6841 ttttttcatg gccccgcgcc tcgccgcccc tacgcctacg tgcgacccga ccgtccgatg
     6901 cttcgacacc tgcccacccg ccttgccgcc tgcctgctgg ccgcgctctg cgcggcgccc
     6961 atcagcgcct cgggcaggcc ggcggcgcag cgccatgccg ccaggccggc cgagccgccg
     7021 cccatccgta tcggcgagat caacagctac aaggctcgcc ccgagctgct cgaaccctac
     7081 cgcaagggct ggcagatggc gctggacgag atcaaccagc aaggcggcgt gctgggccgc
     7141 aagctgcagg tcatcgtgcg cgacgacggc ggcacggccg aaggcgccct ggccgccgcg
     7201 cggcaactgg ccgagggcga gcaggt
//
LOCUS       DASWPO010000119         6168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2615478, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000119 DASWPO010000000
VERSION     DASWPO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615478"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VGC69_06805"
     CDS             complement(<1..120)
                     /locus_tag="VGC69_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0295027.1"
                     /translation="MELNGADIVVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQD"
     gene            615..2816
                     /locus_tag="VGC69_06810"
     CDS             615..2816
                     /locus_tag="VGC69_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEY0295028.1"
                     /translation="MDLRLNDLRRFLYSHYFFNGLRQAIGLLLPVVVMGGVFGEYRLG
                     MVATFGAQCLAIIDQPGSPRRHRANELISGMVLGGLTTAITGLASSHPVLIWLAVVAQ
                     FFAISMLSVYGRRGVLVGFGTLLLMTLSMHTPMAPHEVLAYTLASLAGSFSYFVFSLV
                     MWRVFALQDERKSLAVALFATADYMAVRANFYDETSDLDATYRQLIKRQSTMIERQQA
                     ARDHILRELPSGRGRSDLQRTVLWNTFLDMLRLVDTLVATRTDYAVLRRALAGHDALV
                     FMRDALAKLALGVNQIAGDISRTSSTQYRSGVKAELRAIEYEIEQLRRPGQPQPDHEV
                     ISVMVQVLRRLRNAANIVDRMAAATQARPDTQPASDLRIDPALTRFLSKPVLRLGMIT
                     SNLRLDSPYCRHALRVTLAAALGMLIINFRHGDNLGAHSYWVLLTIAVVMRPGFALSR
                     QRNIFRLAGTLIGCILGPAIFYLTGKPAVYFALLLFSSFMWLSLLQVNYLLSSLFNTL
                     YVVLVFHFVDPGTMSLNVAGERALDTMLGCALALVCSYVLPWWEARYIRPLAHAATRA
                     NLAYLTAGLRYLRAMRHQSDGAAWGDSTAALHATDVTESDLAWRLARKNMQIAFSNFA
                     EAFYRMMREPQQHQVHVPALNSLLTESHILTSQIASVIPTMIDLKEIPDSVDQALAGM
                     AQALDENNNPPPPLPSTIEAEGEAMALIYPIKQMITACTQIRQDLATLDAS"
     gene            complement(2829..3599)
                     /gene="yaaA"
                     /locus_tag="VGC69_06815"
     CDS             complement(2829..3599)
                     /gene="yaaA"
                     /locus_tag="VGC69_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEY0295029.1"
                     /translation="MLFLLSPAKKLDYDTPVQVQAHTQPLFVDRAAALIKVLKTKSAQ
                     DISELMSLSPALSELNVARYAAWKPKFTQDNSRQAALAFNGDVYEGLRAGSLSARQLE
                     WAQEHVVILSGLYGALRPLDLMQPYRLEMGTRLATSKGGNLYEYWGSAISGYLNERQA
                     DAKAPVVINLASEEYFKVVDLKALEARVVQCVFQDWKNGAWKIISFHAKRARGLMARY
                     AIEHKINKPEGLQGFDSEGYAFDAQASSADKLVFRRKA"
     gene            3852..3942
                     /locus_tag="VGC69_06820"
     tRNA            3852..3942
                     /locus_tag="VGC69_06820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3886..3888,aa:Ser,seq:cga)
     gene            4120..4902
                     /locus_tag="VGC69_06825"
     CDS             4120..4902
                     /locus_tag="VGC69_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0295030.1"
                     /translation="MPLTDTACRQAKPQEKPYKLSDGGGLYLLVNQKGKYWRWDYRFE
                     AKRKTMALGVYPDVPLAAARQAHKEARQVLAGGTDPMGERKAKKEDFTFEAIARQWYA
                     HWSPSISVDHADYVLRRLEADVFPEIGSKRINDLSAVNFRDVAKKIENRGALDIAKRS
                     LQTCGQIMRYAVTHGLAERNPVSDIRPSDVIKTQKKRNYSRIDAKELPNLLREIDNYS
                     YYSKLAGAAAGATAGIKIGEIIDTKILAASRCLECGYAFHPG"
     gene            complement(4915..5160)
                     /locus_tag="VGC69_06830"
     CDS             complement(4915..5160)
                     /locus_tag="VGC69_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0295031.1"
                     /translation="MARVSGKQDKNQDLIRLGNAVRDRRMALSWSQETLADYAGIDRS
                     HMGKIERGERNVTFLNILRIAKAIQCKPSDLLQDAEL"
     gene            5280..6053
                     /locus_tag="VGC69_06835"
     CDS             5280..6053
                     /locus_tag="VGC69_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295032.1"
                     /translation="MSDQRCEKHGQHFSIGVRDEIATLRFSSMRHLRGLLLVLPVLFL
                     SACSDQPSTSDVQDSFSERNGLVGCDIFKTKFEKINGVQRDERHYVMEMKGEIVMKPF
                     KKNLAYASELGSYLQDNKDAFEKASAEVKKLVLNYDAAGKIRQQMEGQLSPEQKQVLY
                     RPYEKEKVAQALNIDLGLYSRVYGFYQMRTDTDPELAPYMALYYQDHKDELDALEKDQ
                     KLINEYNAHKVVRQYASSWKTEFIKNIKQECRGCQKFRV"
BASE COUNT         1256 a         1882 c         1815 g         1215 t
ORIGIN      
        1 gtcttgcttg aagattgcgt cgtagatgta aagcactgcg ccgccggggt agccgaaaac
       61 gtgttccacg ccttcttcgg ccaggcagcg cacgacgata tcggcgccat tgagttccat
      121 gatgaatctt tcctttcttt accggccctg gtttcctggc cgttgcgccc gatcagcgta
      181 tggctgggcg gcaactgggc gtgagcagga tcaggcaaca tgcacggcgc tttgcggttc
      241 acggaaccgc gccacccgga caccttgccg acccggcccg cgcccgtcag gatcgcagtg
      301 caatatcagg tcgaaatgga agccttggcc cgtccacccc ggtaagggtg catgcgcttc
      361 tggcgcggcc aacggcaagc aatgctggaa ggaagaagcc ggcaggttgt agccggctga
      421 cgccttctgc gcgccctggg ctggtgtgcc cccggacgca ggaaccgtga actgtacagg
      481 gttgccgctg tcttagcaaa cagtaagaaa acagatgcgg cgcctgcgca accccgcata
      541 ttccccggta atccgacccc aaaaatcatg ggcggcctct ctatactgtg gccgtcccgc
      601 cttgatgctc cgccatggat ctacgcttga acgacctgcg ccgctttctc tacagccact
      661 acttcttcaa tggcctgcgg caggcgatcg gcctgctgct gcccgtggtg gtcatgggcg
      721 gcgttttcgg cgaataccgc ctgggcatgg tggccacctt cggcgcgcaa tgcctggcca
      781 tcatcgacca gcccggcagc ccccggcggc accgcgccaa cgagctgatc agcggcatgg
      841 tgctgggcgg gctgacgacg gccatcacgg gcctggcgtc gagccacccc gtattgatct
      901 ggctggccgt cgtggcgcag ttcttcgcga tctcgatgct gtcggtgtac ggccggcgcg
      961 gcgtgctggt gggcttcggc accctgctcc tgatgacgct gtccatgcac accccgatgg
     1021 cgccgcacga ggtgcttgcc tatacgctgg cctcgctggc gggctcgttc agctacttcg
     1081 tgttcagcct ggtcatgtgg cgggtgttcg ccttgcagga cgaacgcaag tccctggccg
     1141 tggcgctctt cgccaccgcc gactacatgg ccgtgcgcgc caacttctac gacgagacct
     1201 ccgacctgga cgccacctac cgccagctga tcaagcgcca gtccaccatg atcgaacggc
     1261 agcaggccgc gcgcgaccac atcctgcgcg agctgccgag cggccggggc cgcagcgacc
     1321 tgcaacgcac cgtgctgtgg aacactttcc tcgacatgct gcgtctggtc gacacgctgg
     1381 tcgccacccg caccgactac gcggtcctgc gccgcgcgct ggccgggcac gacgccctgg
     1441 tcttcatgcg cgacgcgctg gccaagctgg ccctgggcgt gaaccagatc gccggggaca
     1501 tctcccgcac ctcgtccacc cagtaccgca gcggcgtcaa ggcggaactg cgcgccatcg
     1561 agtacgagat cgagcagctg cgcaggcccg gccagcccca gcccgaccat gaagtcatca
     1621 gcgtgatggt gcaggtgctg cggcggctgc gcaatgcggc gaacatcgtc gaccgcatgg
     1681 cggcggccac ccaggcccgt cccgataccc agcccgccag cgacctgcgc atcgacccgg
     1741 ccctgacgcg cttcctgtcc aagccggtgc tgcggctggg catgatcacc agcaacctgc
     1801 gcctggactc gccgtactgc cgccacgcgc tgcgcgtgac gctggcggcc gccttgggca
     1861 tgctcatcat caatttcagg cacggcgaca acctgggcgc gcacagctac tgggtgctgc
     1921 tgaccatcgc cgtggtcatg cggccgggct tcgccctgag ccgccagcgc aacatcttcc
     1981 ggctggcggg cacgctgatc ggctgcatcc tggggcccgc gatcttttat ctcaccggca
     2041 agcccgcggt gtatttcgcc ctgctgcttt tctcgagctt catgtggctg agcctgctgc
     2101 aggtcaacta cctgctgagc tccctgttca acaccctgta cgtggtgctg gtgttccact
     2161 tcgtcgaccc cggcacgatg tcgctgaacg tggccggcga acgcgcgctg gacaccatgc
     2221 tgggctgcgc cctggcgctg gtctgcagct acgtgctgcc ctggtgggag gcccgctaca
     2281 tacgccccct cgcacatgcc gccacgcggg ccaacctggc ctacctgacg gccggcctgc
     2341 gctacctgcg cgcgatgcgc caccagtccg acggcgccgc ctggggcgac agcaccgccg
     2401 ccctgcacgc caccgacgtg accgagtccg acctggcctg gcgcctggcc cgcaagaaca
     2461 tgcagatcgc cttcagcaat ttcgccgaag ccttctaccg catgatgcgc gagccccagc
     2521 agcaccaggt gcacgtgccg gcgctcaaca gcctgctcac cgagagccac atcctgacat
     2581 cgcagatcgc gtccgtcatt cccaccatga tcgacctaaa ggagattccg gactcggtcg
     2641 accaggcgct cgcgggcatg gcgcaagcgc tggacgagaa caacaacccg ccgccgcccc
     2701 tgccctcgac gatcgaggcc gaaggcgagg ccatggcgct gatctatccc atcaagcaga
     2761 tgatcaccgc ctgcacccag atccggcagg acctcgccac gctggacgcc tcgtagcgcg
     2821 agagggattc atgccttgcg gcggaacacc agcttgtcgg cgctggaggc ctgcgcgtcg
     2881 aaggcgtagc cttccgaatc gaagccctgc aggccctcgg gcttgttgat cttgtgctcg
     2941 atggcatagc gcgccatcag gccgcgggcg cgcttggcat ggaagctgat gatcttccag
     3001 gcgccattct tccagtcctg gaagacgcac tgcaccacgc gcgcttcgag cgccttgagg
     3061 tcgaccacct tgaaatactc ttcagacgcc aggttgatca ccaccggcgc cttggcatcg
     3121 gcctggcgct cgttgagata gccggagatg gccgagcccc agtattcgta cagattgccg
     3181 cccttgctgg tggccagccg cgtgcccatc tcgagccggt agggctgcat caggtccagg
     3241 ggccgcagcg cgccgtacag gccgctcagg atcaccacgt gctcctgcgc ccactccaat
     3301 tgcctggccg acaaggaccc ggcccgcagg ccttcgtaga cgtcgccatt gaacgccagg
     3361 gccgcctggc gcgaattgtc ctgcgtgaac ttgggcttcc aggccgcata gcgcgccacg
     3421 ttgagctcgg acagggccgg actcaggctc atcagctcgc tgatgtcctg cgccgacttg
     3481 gtcttgagca ccttgatgag cgcggccgcc cggtccacga agagcggctg ggtgtgcgcc
     3541 tggacctgta ccggcgtgtc gtagtcgagc ttcttggcgg gagacagcag gaacaacatg
     3601 aagggattcc tcgggttcga atgaaaaaga cacggcaaac cgtgcgccgc ccgctcggcg
     3661 gcgacggtag ttggagtgta gcggcaggcc cgcccggatg gccgctccag aagggttttc
     3721 ctggatggga gatttcaggt agaatctcgg gctttgctgc acctgctgcg cgttttaagc
     3781 acagtgcagc aagaaacgaa aaccgcctgc cttatcgacc acactcgagg cacagcgggg
     3841 agcagtgccc cggagaggtg gcagagtggt cgaatgtacc tgactcgaaa tcaggcgtac
     3901 ggtaaccccg taccgtgggt tcgaatccca ccctctccgc caaattcttg tctagtaccg
     3961 accacgctag actacagaac ccgcaccgct ctaggcgctg cgggtttttt catgtcttcc
     4021 actgtctacc gacgatcatt gtcagctacc gtcacatgac ggtacctttg gcggtatcca
     4081 ccgataccgt catgccagat accgtcagga gggctaacta tgccgttgac tgataccgcc
     4141 tgtcgtcaag ccaagcccca ggaaaagcct tacaagcttt ccgatggagg cgggctgtac
     4201 ctgctcgtga atcaaaaagg gaagtactgg cgctgggatt accgattcga ggcgaagcgt
     4261 aagacgatgg cgttaggggt ctacccagac gtacctttgg cggccgctag gcaggcccac
     4321 aaagaggcca gacaggtatt ggctggggga actgacccta tgggggaacg caaggctaaa
     4381 aaagaggatt tcacctttga ggcgatcgct cgtcagtggt atgcgcattg gtcgccctcg
     4441 ataagcgtag atcatgccga ttacgtactc cgtcgcctag aagctgatgt gttccccgag
     4501 atcggaagca agcggataaa tgacctctct gctgtcaatt ttcgcgacgt ggcaaagaag
     4561 attgaaaacc ggggagcatt ggacatcgcc aaacgatctt tacagacgtg tgggcagatc
     4621 atgcgttacg ccgtaactca tggattagca gaacggaacc cagtttccga cattcggcct
     4681 tcagacgtga tcaaaacgca gaaaaaacgg aactattcgc gcattgatgc aaaggaactg
     4741 cccaacttgc tacgagagat cgataactac agctattaca gcaagctagc cggtgcagcg
     4801 gccggcgcaa cagcaggcat caagattgga gaaatcatcg atacgaaaat tctcgccgct
     4861 tcccgttgtc tcgaatgcgg ttatgcgttc catccgggat agatcgatga aggttcaaag
     4921 ctctgcatcc tgtagcaagt cggagggctt gcactggata gccttggcaa tgcgaagaat
     4981 gttcaagaag gtcacattac gttcgccgcg ttcgatcttt cccatgtggg aacggtcaat
     5041 gccggcgtag tcggctaagg tttcttgtga ccaagacaag gccatgcgcc gatcacgcac
     5101 ggcgttccct aaccggatca agtcctggtt cttgtcctgt ttgcctgaga ccctcgccat
     5161 agagcgaatc gtgccgctat gatgctcaga cgaccacggc atttacgacc ctaacgggcg
     5221 aatgcgttgg tctgaacaga gcgatttcgc ttataggttc ttaccaaaag gaaaaaaaga
     5281 tgagtgacca gcgctgcgag aaacatgggc agcatttcag cattggagtc cgggatgaaa
     5341 ttgctacgct aaggttctct tccatgaggc atctccgtgg ccttcttctt gttcttcccg
     5401 tgctgttctt gtcggcttgt tcagatcaac cgagcaccag tgatgttcaa gatagctttt
     5461 cggaaaggaa cggtctcgtt ggatgtgata ttttcaaaac aaaattcgag aaaattaatg
     5521 gcgtccagcg tgatgaacgg cattacgtga tggagatgaa aggcgagata gtgatgaagc
     5581 ctttcaagaa aaatctggcc tatgcaagcg agcttgggtc ctaccttcaa gacaataagg
     5641 atgcttttga gaaagcatcg gcggaggtta agaaactggt actgaattac gatgccgcag
     5701 gaaaaatacg tcaacaaatg gaagggcagc taagtccaga gcaaaagcag gtcttatatc
     5761 ggccatatga aaaagaaaaa gttgctcagg cgctgaatat tgatcttggc ctgtattctc
     5821 gggtttacgg tttttatcag atgcgtacgg acaccgatcc agagcttgct ccatatatgg
     5881 ctttgtacta tcaagatcat aaagacgaac tggacgcttt ggaaaaagat caaaaattga
     5941 tcaatgaata caatgcgcac aaggttgtta ggcaatacgc cagttcatgg aagacggaat
     6001 tcatcaagaa catcaagcag gaatgccggg gttgtcagaa attccgtgtt taaggcgggt
     6061 tgatattcac acggaaggct ggacgaatcg gtcttcgtaa aggatagcga actggttcat
     6121 ggctgtcttc cagtcatggg cggctcggct ccagttggcg gtgatatt
//
LOCUS       DASWPO010000120        43674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2615506, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000120 DASWPO010000000
VERSION     DASWPO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 43674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43674
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615506"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..502
                     /locus_tag="VGC69_06840"
     CDS             128..502
                     /locus_tag="VGC69_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HEY0295033.1"
                     /translation="MHIWVDADACPAVIKDILYRAAQRWQRPLTMVANQMLRTPPSPL
                     IRAVQVLDKNASVLNPRGERYTADTIRERLSMRDMMEELRSAGVDTGGPATFSQADRR
                     AFANALDRLMVAQARSDASAQG"
     gene            710..1765
                     /locus_tag="VGC69_06845"
     CDS             710..1765
                     /locus_tag="VGC69_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295034.1"
                     /translation="MLGLAAASLAQSPAKPLKSVSIALGTQVVNVTYPWLTLPIALGY
                     WRDEGYDVKIVTVGGSLQGIQQMVAGGVQFAELNSTGLIQANTDNDVAVRGIMGTGII
                     DWGVGVLDNGPIKTVADLKGKKIGIFSLGTGGMPLLKGYLRSNDINPDTDVQIIATGA
                     GAPALEALRADRVQALMFWGSALTGFENAGTKLRVLADPAWRKMPDFTFGALQKTIDS
                     DPKMVEAIARGAAKATLYAWTNPECVRQIHWKNFPSTKPTGADEATLAKWDLALLQAQ
                     LDSMKAAHEMNGGKLIGAMDPAAYGRMQDFMREQGMIKRTVPIESVLDLKPGFMEAVN
                     DFDHNAVIEAAKACQGL"
     gene            1762..2556
                     /locus_tag="VGC69_06850"
     CDS             1762..2556
                     /locus_tag="VGC69_06850"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295035.1"
                     /translation="MNIASPAQTPAFRFVAVNKTFRSKDGGDVIALRDISFEVARGEF
                     VTVVGPSGCGKSTLLRILAGLDRASAGGVELAGKPLAGPSRDIGVVFQAPVLLPWRNV
                     LDNVLVPAQIQGRDAKTARERARHYLELVGLAGFETKYPGELSGGMQQRVGIARALVN
                     DPMLLLMDEPFGALDAMTRETMNMELLKLRERTGATIMLVTHSIPEAVLLGDRVVVMS
                     PRPGRITQIVEVELPARRTLDLINTAHFGGHVSSIRADLHASGGID"
     gene            2556..3377
                     /locus_tag="VGC69_06855"
     CDS             2556..3377
                     /locus_tag="VGC69_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295036.1"
                     /translation="MSTVNPGHSAMDQWLTPLRRRVLVTVFFVAMIAAWHVAVTAFHV
                     SPLIFPGPAGVIRALYDGLVSGEIIHHLWVTLYEILAGFAVGSLVGFVLGALIGQSAL
                     LEVVLYPYVVAFQAVPKVAVAPLFVLWFGFDTSSKVLITATIVFFPVVANTIVGLRSA
                     PRDQIDLMRAFTASPWHVFRMVRLPHALPYVFAGLDIGIVLAVIGAIVGEFVGAQAGL
                     GYLILQRNFSMDAAGMFAILIVLALIGIALHSIIHAIAQRLVFWADAFSDLSRGT"
     gene            3414..4421
                     /locus_tag="VGC69_06860"
     CDS             3414..4421
                     /locus_tag="VGC69_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEY0295037.1"
                     /translation="MTQSEYTLDAPRRASADELPILDLGDWLAGGPIEPLVEQFKSAC
                     TNMGFFYVKNHGIPQSVIDGVFEATRRYFSFPEEERLKDKIDERFRRGFMPMGVTQHP
                     GHKPDLKESYEVGLDLPITDPDVQAGRPLHGPNRWPADKPWFRPAAMDYFDHTMALGM
                     KLLRVFAAALAQPEDFFLQYTKKPMVQSRLFHYPPQTSPTNLELGAATHTDYGMLTLL
                     VQDPIGGLELRTRDGEWVAAPYVEGTLVVNLGDLVKVWTNDRYVSNPHRVANRTGKER
                     YSIPMFFNLDYDTPVACLPQFQSADNPPKHAPIKSGDYLVKRFAAVQKYKTPAELAAA
                     Y"
     gene            complement(4436..5179)
                     /locus_tag="VGC69_06865"
     CDS             complement(4436..5179)
                     /locus_tag="VGC69_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY0295038.1"
                     /translation="MLKSGQENGTLTDRVEALLRTQITGGGFSAGDVLPPENLIAERL
                     GVSRTVLREAVSRLKVDGLVNSKQGVGLVVLDNRRSSVLRLHAADHDDIDELMATVEL
                     RTGFEVEAAGLAAQRRDQADLDAMNQGLLDMRRALDAGDVASGVDADFAFHRAIAEAT
                     RNINYIRFFEFFTELYKANLMESRSRSAKAARGEHAQAEHQAIYEAIQQGDPDRARAT
                     ARAHLDNTAARLKTSNGLLRPTGRLAGGA"
     gene            5364..6713
                     /locus_tag="VGC69_06870"
     CDS             5364..6713
                     /locus_tag="VGC69_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0295039.1"
                     /translation="MRPIEELVRADQGLVSREIFTSEALFQYELKYIFRRAWLMVGHE
                     SLIPKPDDYFVSRMGKDSVIVTRDRVGQIHVLLNSCAHRGMKLCRYDSGNNRAFSCPY
                     HGWSYSTDGKLVEAPGGLVGVPGYDTYYKKELDKNAWGLKRVPSVHNYKGTIWASWDP
                     DAPPLLDYLGGMKIYLDSLLDHRDGREGGSEVVGGIQKWRVGCNWKFPPENFIGDKHH
                     DISHRSADLVGVGPSPGKGRRDLTSKRMCVAFPDLGHGAIARLPHFEEFDYVPQYKGV
                     PVLEEYFEQVHQDRVRNLGHKMRVTPHATATVFPNMSFHALQPRSIGLFHPISPTETE
                     IWRIYLTDADAPPEVKAATHHYNISYHGPAGMAESDDMENWSCATESCIGPTAGEMDF
                     NYQMGLGHAEKVPHLNDAVYSDFSEEGARAFYRQWGRFMQRLDWDELRPNARKEAQR"
     gene            6710..7240
                     /locus_tag="VGC69_06875"
     CDS             6710..7240
                     /locus_tag="VGC69_06875"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HEY0295040.1"
                     /translation="MNHPAPQDDMQLWWQVQQFYATEAALLDERKYEDWLGLLHPDIA
                     YTVPIARNVRRDRLAEEYTHAGETCWFDEGIVLLTKRVAQLKTGMHWIEEPTSRYCRL
                     VNNVRVLGFDEASRAFEAESKFLIYQNRLEAEVAILAGTRVDRLVREGSSFRILKREV
                     FLAQSTLLAKALATFF"
     gene            7277..8302
                     /locus_tag="VGC69_06880"
     CDS             7277..8302
                     /locus_tag="VGC69_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295041.1"
                     /translation="MNTIQPSSCNTTRRSLLIAAGLAGLGTAPGLLAATRGYPDHPVR
                     IILPGPPGLALDIVVRVVADALGAKYNQSFIVEDRPGAGGMVAMQMYANNYKPDGYTL
                     LAGGLGPSVMIASLFKNVPLDPYKAFIGVGQMAESANILVVRADLPYQSVAELVAAAK
                     ARPNEIKVGSNDIGSSMQMSYELFAQRTGTQYIYAPYRGPNEVMGGLLSETIDVGFSS
                     FGPFVPTVKSGKLRALAVTTSYRQDMLPDVPTMQEAGVHDYDVGSWLCLHALPGTPAA
                     IIDQLGRDMVEIVNRPEIGEKLKAAGYMPRPLEAKAFQEKWAKEMTRWTAVAQNAHLV
                     KDYQTGT"
     gene            8323..8523
                     /locus_tag="VGC69_06885"
     CDS             8323..8523
                     /locus_tag="VGC69_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295042.1"
                     /translation="MAFDKSINLLDLQPGARVKDAEGVVFQVVDNPGDGMWVICKALQ
                     ANGALAEEDSPLFAGNIVEVLG"
     gene            complement(8520..9482)
                     /locus_tag="VGC69_06890"
     CDS             complement(8520..9482)
                     /locus_tag="VGC69_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0295043.1"
                     /translation="MYPAPPFPVGTSVDLNGERRRWFSYQDIENIALHHRREVMACRY
                     DAIVGLARGGLYLAAMLSQMTDIALGTAHYDRAAGTVQLHHLPAGRPARVLLAEDVSG
                     KGHTLERTKHALEQRGANVDLMVVCWDALSRLAPDYYGAKLEAGERYLFPWERSQLAP
                     TSPDRVSHTDIANWLTAFDLDGVFVEDVPETLYAADLDAALAARDQLPALPPHPYWRN
                     GGLIISGRLEADRARTEAWLGRHGLAPSRLLLRPSTGIPTPEFKRRALIDLSVTEYIE
                     SELAQAQHIAGLPHVVVWHYDCHTRKNRRITGRAELSPAPGGRG"
     gene            9546..9947
                     /locus_tag="VGC69_06895"
     CDS             9546..9947
                     /locus_tag="VGC69_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295044.1"
                     /translation="MHSSLPLRPACPPGACACGREALLADSAGDKRILMLTKAEENKL
                     VERLESVASLAELRRMQVKMQDLLGIVVHIAPGPNEVRTVRGFSIRIEEMPGLCSKTK
                     QTIPAAIRRCLDQRPEIAYAILNEHDLLSGT"
     gene            complement(10015..12501)
                     /gene="mdoH"
                     /locus_tag="VGC69_06900"
     CDS             complement(10015..12501)
                     /gene="mdoH"
                     /locus_tag="VGC69_06900"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY0295045.1"
                     /translation="MNPMPSEPEGLIHDYIQRLPLTEAQRQTLERSVQQAVSRGEAPW
                     MALHEAIGQHTTVTEPSMASEGQRLMLGYGAGPLAAGVMHSDASGAEIAAMPPHVRTP
                     LAPVPWVPNPLVRVWRRLRADRPQDPPETLAALHANGDGSAWRLAGSRRRLALLVGVG
                     VQTAVATWYMKGVLPYQGGQVLEVLILILFALLFGWVSSGFWTAMMGFLQLMIGRDRY
                     SVSARGLSNDPLPEGARCALVMPICNEDVARVFAGLRATYESIARTDSLKNFDVFVLS
                     DSYQADICIEEQKHWLELCKAVGGFGRIFYRRRRRRVKRKSGNIDDFCRRWGAHYQYM
                     VVLDADSVMTGTCLKSLVQLMEAHPDAGIIQSAPQASGMDTLYTRVQQFATRVYGPLF
                     TAGMHFWQLGESHYWGHNAIIRLQPFMEHCILAPLPGKGAFAGAILSHDFVEAALMRR
                     AGWGVWIAYDMPGSYEELPPNLLDELQRDQRWCHGNLMNFRLFLIDGFHPVHRVVFLT
                     GVMSYLSAPLWFLFLALSTALLAVHTLTEPQYFVEPRQLFPIWPQWHPDKAIALFSTT
                     FVLLFLPKVLGLLRVWFRDPADYGGRIKALQSMLIEVLFSMLLAPVRMLFHTRFVMAA
                     FLGLKAKWVSPHRDAQDTPWGEAIRRHYSQTLLGIAWATLVGWLNPVFLWWMVPILAA
                     WLLIIPLSVYSSRLGPGLKTIQHNLFRIPEEMRPPKELEDTHLYTHAAREHSAGEPTF
                     GDAITDPVTNALVCAMGTARHRPVPANLTARKALVDLAVEHFAGLSEPQRLKLLDDPI
                     AMAQLHRLAWSKAETARDDTGSASKMAAFA"
     gene            complement(12482..14386)
                     /locus_tag="VGC69_06905"
     CDS             complement(12482..14386)
                     /locus_tag="VGC69_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEY0295046.1"
                     /translation="MTFSIANRAFRGSISLGSLVFATLLTGTSYAQSQPAAPVVDTTH
                     AEVIRIEVPPEPEQPAAKPEAPKPKPKPKPADAKPAESKPADAAPEAAKPDTAKPADA
                     AKPAGAAKPEAAKPEPKPAPAAKATPNKAEQEDSKAGFTFFEVSKQARDLAAQSYKQP
                     ASNLPTELQDLKYAGYQNVQLRDDRTHWRDDGTPFRLGFYHQGMHFNTPVAINEVDEQ
                     GHSHPINFDPDDFNYGDLKVDPNLLKNLGFAGFKVLYPVNTALKPNDELASFLGASYF
                     RVIGRGQTYGLSARGLAIDTALPSGEEFPAFRKYWIIKPAKGARELVIYALLDSPRAT
                     GAYRFTIRPNIDTVVDVKARIYLRAKVGRLGLAPLTSMFLYGANQPSPTQNYRPEMHD
                     SDGLAMHTGNDEWLWRPLNNPRKLAVSAFNMQDPRGFGLLQRTRDFHHYEDLDDHYEK
                     RPSLWIEPVGDWGKGSVQLVEIPTPDETNDNIVAFWVPDDQPAVGTPLDIEYHMTWTL
                     DEPRTHTTPLAWVMQTRRSRDEVKGPDLIRHGDGSTAFIIDFVGSSLKNLPADMVISA
                     DVSADANGQIVENTVRPNPITGGRRLTLRVNVKDPTKPVEMRATLSNGQIPLSETWSY
                     QMPGNEPDAK"
     gene            complement(14595..15359)
                     /locus_tag="VGC69_06910"
     CDS             complement(14595..15359)
                     /locus_tag="VGC69_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295047.1"
                     /translation="MLLFLVRFLFGFALSLVSALLLAVATWLCLKLIALAMNLFAERR
                     TQDMAGALIVLTVIAAALAALYGAGHALYGYMGAWRAVGQAPGWLAGPRLISPEAILG
                     ALAGFGGLAAIPGAWRWRAQLAAAQRDEQPAKPRAAEDKAAKPTKAAKSSAKPAATAA
                     SARAAAKAAAPAPKRPTIPQLGWLALFLLALGGVLLAFVVIVAPLTPAGSLAATLRGA
                     GRLLPIYVSALALFGCGLLCLLLWRVCRRAPAAASR"
     gene            complement(15405..16487)
                     /locus_tag="VGC69_06915"
     CDS             complement(15405..16487)
                     /locus_tag="VGC69_06915"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY0295048.1"
                     /translation="MKTAADSILLASLNLSLPIIQAPMAGTSTPALAAAVCNAGGLGS
                     LGVGAMDAAAAREAIRQTRALTAGAFNINLFCHAPAQADAARERAWLDYLAPDFAEFG
                     AKPPAALREIYTSFVADEDMLQMLLDERPAVVSFHFGLPEAPRIEALRAAGCYLMATA
                     TSLAEARRIAAAGLDAVVAQGIEAGGHRGLFDPEGPDEQLGILALTRLLAARLPLPVI
                     AAGGIMDGAGIAAALALGAQAAQLGTAFASCPESAADAAYRQALLSQDDSHVTVMTRA
                     ISGRPARGFANRLTALGTAPGCPPLPDYPIVYDAGKALHAAAKARGSADFAAQWAGQG
                     FRLSRGVPATALMRRLQDEMAATSMC"
     gene            16547..17707
                     /locus_tag="VGC69_06920"
     CDS             16547..17707
                     /locus_tag="VGC69_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0295049.1"
                     /translation="MALRQSSFFIRFVILGVLVHVYVGLRLITDAPVDAAAAAGGWLL
                     LVASCVLIPMGMLSRRSSQRRPWGDLFAWAGLLVMGFFSSLFVFTLLRDAVLLFAWIA
                     SLLGASVPMLRLREISSLLVPALALLGSVVGFFNARRLARVVTVNVPVAGLPEALENF
                     TIVQISDVHVGPTIKSDYVRAIVAAVNAERPDLIAITGDVVDGSVDMLLAHAQPLSGL
                     HAPHGVYVVTGNHEYYSGASAWVREFRRMGLTVLMNEHVVIEPAGQAMVVAGITDYTA
                     GSFDEAQRSDPARALAGAPADAVVKLLLAHQPRSAMEAAPLGYTLQLSGHTHGGQFFP
                     WGFFVQLQQPYTAGLHRLQGMWVYTSRGTGYWGPPKRLWAPSEITRIRLVRG"
     gene            complement(18233..19411)
                     /locus_tag="VGC69_06925"
     CDS             complement(18233..19411)
                     /locus_tag="VGC69_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HEY0295050.1"
                     /translation="MTSTSSLSSPTPPDTAGMGPLLRLALALFLSYLAVALSLSAMSV
                     HVAHGIGLGNTLSGLAVGIPFLSTILTRGYAGNYSDRLGGKACAMRGLVVYIVAGLIC
                     LASDQFGLGPWPAYGVLIAGRLLLGLGESMALVGIISWGMDMMGPARSGQVLAMIGMA
                     MYGAFAVGGPLGLAMLDASGFSALMLLCAALPLVGLLVALGLPAIAPHAGQRASFWQV
                     LGQIRREGTVVGLQGVGFAVIGAFIPLHFLAKGWPHAGLGLSCFGLGFVLVRILFGRL
                     PDKLGGRPVAIWSMGIEAAGQYLLWQAPSPALALAGALLTGLGCSMVFPAMGLEIVRR
                     MPPQLRGAAMGGFAAYQDIAYGATGPLAGLLADHFGYGSVFLAGALCATVGFGLAVRR
                     "
     gene            complement(19623..20954)
                     /locus_tag="VGC69_06930"
     CDS             complement(19623..20954)
                     /locus_tag="VGC69_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0295051.1"
                     /translation="MLHAPILPMLLRMGWPNMLMLLAQSGAALVETWYISHLGTSALA
                     GVALVTPLLMLMQNMSQGAMGGGISAAISRALGAGRQDMADQLVLHAVLINAALGLVF
                     TAVFLLEGTRIYTLLGASSGALDAALTYSDVLFGGLVLMWIMNALASVVRGTGNMLVP
                     GAVICGGALLVVPVSPCLIFGLGPIPALGIAGGAWTLLAYFAGGTLVLGWYCLSGRNP
                     ARLKPTAFQPTLLRQILAVGGLATLNPLLTNGLIATTTALVGAYAGTAALAGYGTSAR
                     LEYLLMPIAFGLGAPMVALVGANIGANQPQRARQAALSGGAIAFVLGEIVGLLAAFWP
                     LAWLHLFGRDAGMEASGSTYLHIVGPFYGFFAMGFSMYFASQGARRLVWPLLAGFVRL
                     AVAVGGGWLVLRAGGGLAGLFTVNAVAMVLYGSIILRAVSARGWIRTRQSP"
     gene            21099..21563
                     /locus_tag="VGC69_06935"
     CDS             21099..21563
                     /locus_tag="VGC69_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0295052.1"
                     /translation="MDATIARESVCNNSALRRAARRMAQWYDEAMAPVGLRGTQYTIL
                     LQIDRAGGEPLVGKLAEALVMDLSALGHTLRALQREGLVEVVVDAADRRSRRARLTHS
                     GKARLAKARVQWARAQRRFEAAFGVEDAARLRGVLDFIASPDFDERLRGQAD"
     gene            complement(21560..23041)
                     /locus_tag="VGC69_06940"
     CDS             complement(21560..23041)
                     /locus_tag="VGC69_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY0295053.1"
                     /translation="MNSMQNPATRTPSKNRPAIARLTLALLAAGAIGGAGLSAYTGAA
                     HAQAAPTQATAPIGMMAAPNFAQINKEYGPAVVNVSVSGTTKVSDDEDDPFAQFFGQF
                     PGFPGAVRPHGGGSHEVPMQGVGSGFIISPDGVILTNAHVVKDAKEVTVKLTDRREFR
                     AKVLGSDPQTDVAVIKIQASNLPTVKVGNVSDLQVGEWVLAIGSPFGLESTATAGIVS
                     AKGRSLPDDTTVPFIQTDVAVNPGNSGGPLFNDHGEVVGINSQIYTKTGGYQGLSFAI
                     PIDVAYKIKDQILAHGKAEHARLGVMAQEVNQDLADSFKLDSPSGALIASVEKGSAAE
                     RAGLQPGDVIRKLDGRDIVSSGDLSAVISMASPGQHVSLDIWRNGAHKELTATLGSLD
                     KQSVAADDNGAQAGHGKLGLALRRLTPQEESEVGTSGLVVQQAEGPAARAGIQPDDVV
                     LSINGVPASSVDQIRKEVANSKGTVALLIQRGDDKIFVPVRLG"
     gene            complement(23125..24456)
                     /locus_tag="VGC69_06945"
     CDS             complement(23125..24456)
                     /locus_tag="VGC69_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0295054.1"
                     /translation="MPFPVTSLRARLLLFLLVAIFAAALIQAVVAYRGTLAQTDDIFD
                     AQLRSTALSLSQGDLRLNNAPAPVVPGVPTASVDDLIIQIWTVDGVQLYKSASGRLLP
                     DPVTLGFSTVRAGDAAYRVYAMATPLQVTQVAQDLRVRSLTARNLALRSVGPILAVAP
                     LLMLVVWWVVSRSLRPVARTRAQLAARQPDDLSPVSDAGLPDEIRPLVQELNLLLERV
                     RQAFASQQQFVGDAAHELRTPLAALQLQLLGLRRATDAAAREQAIARLGDGVARATRL
                     IEQMLAMARHDNGAPHLVLSPTDLVQSVREAVSQVLPQANAKRIDIALDGPDSAVVDG
                     QADALVLLVRNLLDNAVKYTPDGGAVRIAVQSGASVALTVDDTGPGIRPSERERVFGR
                     FYRSADAGATGNGLGLALVRTLAGRHGATVTLGDAPDGGLRVRVMFPPVSG"
     gene            complement(24456..25136)
                     /locus_tag="VGC69_06950"
     CDS             complement(24456..25136)
                     /locus_tag="VGC69_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0295055.1"
                     /translation="MRILLVEDDMMIGTSVQDCLRADGYAVDWERDGRRAATALRAGV
                     YDLVLLDLGLPQQDGLSVLRELRARRDRTPVLIATARDAVSDRIAGLDAGADDYVVKP
                     YDPDELLARIRALLRRSAGHAESVFEHGGVRLDPQTHEATVDGEPVTLTAREWAVLQP
                     LITRPGMIYSRAQLEEKLYSWHEEIASNAVEVYIHGLRKKLGHGFIQTVRGVGYVIPR
                     QPGKAGDT"
     gene            25260..26609
                     /locus_tag="VGC69_06955"
     CDS             25260..26609
                     /locus_tag="VGC69_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HEY0295056.1"
                     /translation="MLRPHRTWRRPAAFRAGRRFVPDRLAVMLLAAAVVGVLGALATV
                     LFREALELLQVWLGGHPAQGMVSLARGLPLWERLLLPAAGGVLAGLILQWARRSLPPQ
                     GASDYMEAIAVGRGIIGLRQTVARSLASLCSIGSGASIGREGPMVQLAAMLASLFGRW
                     LVWPPGRLRLLVACGATAGLTAVYNAPIAGALFVSEIVYGAVSSVKLVPLVVASVASN
                     MVTRQLLHYDAVYHLPAFDFASPWEVFSYLGLGALAGLVAPWYLRLLDGSRRLFSHWI
                     GPLWLRLAAGGLVVGAISLAWPEVWGNGYSVVNSILHQPWAWQAVAALLVMKVLATAA
                     SSGSGAVGGIFTPTLFVGAALGHLYGTALHDALPQVSAVSSYAVVGMGALLSAATHAP
                     LMSILMIFEMTLSYEAMLPLMVACVMAYAVVRALGAKSMYAGSLADNRGARRRADGH"
     gene            complement(26634..27254)
                     /locus_tag="VGC69_06960"
     CDS             complement(26634..27254)
                     /locus_tag="VGC69_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295057.1"
                     /translation="MFGQSRRQVFKPSIYQPGRHNRRLPRWLALLGIGIALGAGGVLF
                     LQTNYGPPRLSTEQSEQVRSELNSATLERQRLQAQIDDLQHQLAASRTAQGQTSATTQ
                     AQPATSIAPVEPPAPIAPMTAPAPAAAPSSMPAMPAMPTMPAMPTMPAMPATPALQPP
                     DTVADPSAEPPTTPPAKPAARRPAAPRNYEEPHSELRMTVIPAAPQ"
     gene            complement(27299..28000)
                     /locus_tag="VGC69_06965"
     CDS             complement(27299..28000)
                     /locus_tag="VGC69_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0295058.1"
                     /translation="MQAMILAAGRGERMRPLTDHTPKPLLPVGGKPLIVWHLERLARA
                     GLHDVVINHAWLGERIVEALGDGSRWDTRIRYSAEGQALETAGGIVQALPMLGAEPFL
                     VINGDIWCDWDPAQARAARQRLDASGADAWLLLVGNPPQHPLGDFALQPDGRAAEEGQ
                     ARLTFSGIGVYRPALFAGLVAGQAAPLAPLLRQAMARGAVQAVRHGGRWVDVGTPQRL
                     QALDAELGTNRYSML"
     gene            complement(28000..29040)
                     /locus_tag="VGC69_06970"
     CDS             complement(28000..29040)
                     /locus_tag="VGC69_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0295059.1"
                     /translation="MTNPHDPRQALIDAWLRSLPSGLALRPDTLRAASSDASFRRYFR
                     IETGNGTAIVMDAPPPHEDVGPFLHAGGLLREAGLNVPAVLAQAPEQGLLLLSDLGNE
                     TYYQRIQAGLDDARLQSLYRDALAALVHMQQAAITGLPSYDAARLAAELELFPEWYVQ
                     RHHGASLTDKQAADLRQLLQLLSTHCAAQTPVYVHRDFHSPNLMVCDEPRFGPNPGIL
                     DFQDALAGPLTYDLASLVTDARTTWEEPQQLDWAIRYWDMARQAGLPVDADFAEFHRA
                     YEWMSLQRNLRILGVFARLNHRDGKPGYLAHMPRVNRYVRQVAQRYGVFAPLLRLLDQ
                     LDRTTPTVGYTF"
     gene            29221..31623
                     /locus_tag="VGC69_06975"
     CDS             29221..31623
                     /locus_tag="VGC69_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY0295060.1"
                     /translation="MRMVRWLILLAVGAAGAAQAQAVSGTDASPAAGAAAQPAANQVE
                     VRGIQPAVDLRSTSGLRVHRQTDDKIPAFLEGDRIDGSPDTRLTLTGNAQVRRIDTVI
                     KGDSIDYDRKTGEATVTGHLRLQRDATLVTGPTGHVNVDSDQGEIDKPDFWIGATGGT
                     ARAEHADIFSKSEFRLKQVTYSGCSCAQPSWYIKADSLDVDMDENEGVAHNGVLYFKN
                     VPILASPYLTFPVKKERKSGFLVPMYGMSSNTGIDISMPYYLNLAPNYDVTLTPRIMS
                     KRGDMLSADGRYIGDGYTGMVQGSFLPNDRLADRDRWMYRWTHYQQLGNGFYADWDLA
                     RVSDNNYYRDITTIGLNSATTVYLPQSARVGWNGTYWQTSVQAIRYQTLQDPTSPIAP
                     QFDELPAIRLTGHRYDWNGLDLSMDSSAIRFSRPLLEGTRLSPNGDRVQIYPSVSYPI
                     VRPGWYITPKIGVNYTQYGSTNWYNTDFNGLGSTAPYQASQSRTLPIASIDSGMVFER
                     DTSLFGRSATQTLEPRLYYLRIPYRNQSNLPVYDTYLAPFSFDQAFQENQYAGGWDRI
                     NNANQLTAALTTRWIDNATGLERLSLSAGQQKYFTDQLVTLPGEVARIHQRSDYLAGA
                     SAALTDTLSTEIGAQYNPYSSQWSQAYSSLRWTPQRLATVALTYRYQRNPLPDVPYQP
                     IGQNQISLGVQWPFAKHWYGVGRLDYTIGSEVSDSTSRVPQAIIGVEYKSDCCWSARV
                     VFQRYATSSSQVNTALFFQLELEGLGALGSDPMSLINRSIPGYQPVTPAQQPGTSFER
                     YE"
     gene            31623..33221
                     /locus_tag="VGC69_06980"
     CDS             31623..33221
                     /locus_tag="VGC69_06980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0295061.1"
                     /translation="MRRLSLRGRSSGCILMLTLCVALGASPVHAQNAKPDSSGAAKQS
                     VKAKAPAKKPAAKPQPAPAPAASAGGEQFGDGIAAVVNKEVITMRDLREASQRTAADL
                     QQHKIQVPPDDVLQRQVLQQLIMDRLQEQEARRLGIALTDAQVDQAVGMIADRNRLTV
                     DQLKAEIAKTGASWDGYRKSIREEIRTTRLRQRMVDSNITITDGEVDAFLKEHARAAA
                     AAPAPQPVQPAPAPAQPTVAIVPNGPIALAQILVRVPEGASSEDVDRLRKKAEGLLAR
                     ARKGEDFASLAAANSDGPEALQGGQMGARPIDGWPDLFVQAVRGLRPGQVSGLVRSGN
                     GFHILKVMARAMQQIAAPTQAAPAPAADEDPIPPESSGAVQITQTHARHILIKLSAVM
                     NDEQAREKLDDIRRRIVEGGVSFADMASQYSQDSTAPQGGDLGWLDPGETVRAFEQVM
                     DKLQPGEISQPVASPFGWHLIQVLERRQHDATEDVRRNRARQILLERRAQPAFEDYLD
                     QLRAQSYVDNRLEKQDQLQQRYQQ"
     gene            33306..34097
                     /gene="rsmA"
                     /locus_tag="VGC69_06985"
     CDS             33306..34097
                     /gene="rsmA"
                     /locus_tag="VGC69_06985"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY0295062.1"
                     /translation="MAQHQARKRFGQNFLTDESVVDGIVRAIAPAAGDHVIEIGPGLS
                     ALTRPLLDRLPSLTVVEIDRDLAARLRKQYPAERLTVIEADVLTVDFTTLGEGLRVVG
                     NLPYNISSPLLFHLMAAAGQVRDQHFMLQREVIDRMVAKPGNADHSRLSVMLQSRYRM
                     TKLFDVPPEAFDPPPKVVSAVVRMVPLGEDRPRPRSDAAFEQVVAKAFSQRRKMLRGV
                     LGDWTAHIPWETLGIEPTARAEAVSVVGFIGMADALVAAGVLPGS"
     gene            complement(34129..34965)
                     /gene="fdhD"
                     /locus_tag="VGC69_06990"
     CDS             complement(34129..34965)
                     /gene="fdhD"
                     /locus_tag="VGC69_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY0295063.1"
                     /translation="MPPFPVSDEHDPPASARVAVRRVRGGRIEANPRDDALAEETPIA
                     LEYNGISHATMLATPADLEDFALGFSLTEGIVDGVSDVRGIDLRQECDGIVVELEIAS
                     ACAVRLRERRRAMAGRTGCGLCGVETLPEVLRPVAPVAAGAPADLAAVLQAMRAMRAR
                     QVLHDATGATHAAGWARLDGQMELVREDVGRHNALDKLIGALARAGRDPAQGMALVSS
                     RASFEMVQKAASCGTGILAAVSAPTALAVRLAQQANLTLLGFMRGEDATIYAHAERIA
                     SR"
     gene            35063..36175
                     /gene="moaA"
                     /locus_tag="VGC69_06995"
     CDS             35063..36175
                     /gene="moaA"
                     /locus_tag="VGC69_06995"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY0295064.1"
                     /translation="MSKVIFLADRRAAQPMVHEAASLAWQPGEPLLDAFARPMRDLRI
                     SVTDRCNFRCTYCMPREVFDGSYPFMPHSDLLSFEEITRLAGLFVQMGVEKIRLTGGE
                     PLLRKDVESLIAMLAGLRTPQGRPLDLTLTTNGVLLARKARALKDAGLTRVTVSLDAL
                     DGDMFARMSDSRFTPDDVLRGIDAAAQAGLAPIKVNMVVRRGVNDGQILPMARRFRGT
                     GHVLRFIEYMDVGSTNGWNLAEVLPSSEVLARIAGEFPLQALEGAAMGRVAQRWRYAD
                     GAGEIGVISSVSHAFCGGCTRARLSPEGKLFLCLFASQGYDLREPLRHGASDAQLASR
                     LAGIWSGRSDNYSERRAQSTPEHQAARKIEMSYIGG"
     gene            36226..36822
                     /gene="mobA"
                     /locus_tag="VGC69_07000"
     CDS             36226..36822
                     /gene="mobA"
                     /locus_tag="VGC69_07000"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY0295065.1"
                     /translation="MLGVGGLILAGGRGSRLREADKGWLMLAGLPLVDHVAQRLAPQV
                     DTLLISANRNPERYAQYGQVLADDPLFGAWSGPLAGVAAGLRAWSGPWLVVAPCDTPF
                     LPRDMAARLIDAAQARGAPLAVARAAGRRHSVCMALRTSLADDLRDYLQGGDRKVEHW
                     QTRVGGVEADFDDAPHAFFNINTPEDLAAAALDGNGRQ"
     gene            36860..38965
                     /gene="fusA"
                     /locus_tag="VGC69_07005"
     CDS             36860..38965
                     /gene="fusA"
                     /locus_tag="VGC69_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0295066.1"
                     /translation="MTRRTPIERYRNIGISAHIDAGKTTTTERILFYTGITHKIGEVH
                     DGAAVMDWMEQEQERGITITSAATTAFWRGMAGNYPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYDSVGGVQPQSETVWRQANKYGVPRIAFVNKMDRVGADFFRVQRQIGE
                     RLRGDAVPVQIPVGAEDNFEGVIDLVKMKAIIWDDASQGVRFEYQDIPANLQVQAQQW
                     HDNMVEKAAEGNEALLDKYLSGEPLTEDEIKLGLRLRTIANEIVPMLCGSAFKNKGVQ
                     AMLDAVIDYLPSPLDVPAIKGHSVDDKEIERHASDTEPFSALAFKIMTDPFVGQLVFF
                     RVYSGMVKSGDTIFNPIKNKKERLGRILQMHANARQEISEVYAGDIAAAVGLKDITTG
                     ETLSDPAHVIILERMVFPEPVISQAVEPRTKADQEKMGLALSRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEILVDRMKREFNVEANVGKPQVAYRETIRATVDEVEGKFIKQSG
                     GRGQYGHAVLKVEPQAPGKGFEFVDAIKGGVVPREFIPAVERGIRDSLNAGVLAGYPV
                     VDIKATLVFGSYHDVDSNENAFRMAGSMAFKEGMRRAKPVLLEPMMHVEVETPEDYTG
                     PVMGDLSSRRGMVQGMEDIAGGGGKLVRAEVPLAEMFGYSTSLRSLTQGRATYTMEFK
                     HYAEAPRQVAEEVIAAHKS"
     gene            complement(38972..39778)
                     /locus_tag="VGC69_07010"
     CDS             complement(38972..39778)
                     /locus_tag="VGC69_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0295067.1"
                     /translation="MRFAPDPPASSVDFDGYTTAYAEHGAGTPLVLVHGSLCDWRYWK
                     PQIAGLGRHFRVLALSLRRYWPERWNGEGDGFSIAEHADQLLKFVEQVAGGSAHLVGH
                     SRGGRVVLEAALRNPGLARSLTLADPGQRLPGHADQRGNFRQRARDLIRAGQAEEGLA
                     LFIDAVTGPDTWRRMVPWFKEMVRDNAATLLGQADEILPLPDEHALRALLPPVLLIGG
                     ALSPAPFPAVIDWLERLLPATQRVSIEHASHGMNLSNPRAFNAAIEAFLE"
     gene            39953..40447
                     /locus_tag="VGC69_07015"
     CDS             39953..40447
                     /locus_tag="VGC69_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY0295068.1"
                     /translation="MSAEPNSLPESAQRVALLLQSLGHDKPVVLLEQAGRTSAQAAAG
                     LGCEVAQIAKSIIFRRVSDNAPVLVVASGINRVDEAKVAARVGALGRADARFVREATG
                     YAIGGVCPIGHAIKPVTLLDEDLFRYDSLWAAAGHPHAVFNLTPRQLEAMTGAPVADV
                     ALRA"
     gene            complement(40450..41541)
                     /locus_tag="VGC69_07020"
     CDS             complement(40450..41541)
                     /locus_tag="VGC69_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEY0295069.1"
                     /translation="MKTFHCGQCAQLVFFENVKCERCNSLLGYLPDVGEVGAFEPAGG
                     QAWRALHPQAAGRLYRQCGNYAVENVCNWMIPADSSDALCQACALTETIPNLSSPDNR
                     LLWYRLEAAKRRLLYTLLVLGLPVESRAQDPERGMSFAFKESTDAEPVMTGHADGLIT
                     MNLAEADDATRERIRADMHEPYRTLLGHFRHEIGHYYFDRLVAGTRWLEPFRAQFGDE
                     RADYQAALDSHYANGAPAGWEHEYVSEYATMHPWEDWAETWAHYLHIVDTLDTATACG
                     LVLAPDDESLPTLSDQTSVDEASFGNLIRRWFPLTYALNSLNRSMGMPDAYPFVLAPA
                     VVQKLRFVHNVVASSGATDAEPSLVARSA"
     gene            complement(41596..42279)
                     /gene="ybgF"
                     /locus_tag="VGC69_07025"
     CDS             complement(41596..42279)
                     /gene="ybgF"
                     /locus_tag="VGC69_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY0295070.1"
                     /translation="MRDRATTLRRLALIASLACAAATTPAYAFADDDARRAILELRQQ
                     FEQSQRAQLQLSDQIQALQQQLAQLRGQIEELSRPQPQQQAAGNAQQGAATAPGGPVA
                     DPQEAATYNNAIDLFRKGQYKESSDSLSAFVALYPNSDLAPSARFYQGSSRYALKDFK
                     GAITQLNSLVQDAPNDARAPDALLVIAGSQIELNNRAAAKATLQKLISQYPSTQAAST
                     AKSRLQLLQ"
     gene            complement(42384..42878)
                     /gene="pal"
                     /locus_tag="VGC69_07030"
     CDS             complement(42384..42878)
                     /gene="pal"
                     /locus_tag="VGC69_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY0295071.1"
                     /translation="MKSRIAKSLSLAALTAALAACSSVPLDKNAGAGGGAGGGTSGDP
                     MDPFNPNSVLAQQRSVYFDFDSYSVAEQYRGLVETHARYLAGHQQQHIKIEGNTDERG
                     GSEYNLALGQRRAEAVRRQMTLLGVSDSQIETISFGKEKPKALGHEESDWAQNRRADI
                     DYIR"
     gene            complement(42912..>43674)
                     /gene="tolB"
                     /locus_tag="VGC69_07035"
     CDS             complement(42912..>43674)
                     /gene="tolB"
                     /locus_tag="VGC69_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY0295072.1"
                     /translation="YELQVADADGQNPQVALRSREPIISPTWSPDGARLAYVSFHTGK
                     PVVYVQTLATSAQTPVANFKGNNSAPAWSPDGSQLAVALTRDGLSQIYIVPSSGGSSM
                     RRVTRSPGIDTEPFFTPDGRSLVFTSDRSGGPQIYQTGLDGSEARRLTFNGSYNISPR
                     ISPDGSTLLYVARRDGSFRIASLNLATGSEVLLTDGTDDQSPSFAPNGMQVLYATVQG
                     GRSVLAGVSSDGRVRQTLSVLNGEIREPTWGPFTR"
BASE COUNT         7068 a        14213 c        15242 g         7151 t
ORIGIN      
        1 attcctgatc atcggcgccg tgacgctgct ggctgttccg gtatttcggc gcttgcgccg
       61 cgacgagtgc agcgcgctga gcgggcgcaa ggcctgagtg cctcactgcg tctcgaagac
      121 tggcaccatg catatctggg tcgatgccga cgcctgtccc gccgtgatca aggacatcct
      181 gtaccgggcg gcgcagcgat ggcagcggcc gctgacgatg gtggccaacc agatgctgcg
      241 cacgccgccg tcgcctttga tccgcgccgt ccaggtgctc gacaaaaacg cctcggtgct
      301 caatccgcga ggcgagcgct ataccgccga caccatacgc gagcgcttgt cgatgcggga
      361 catgatggaa gaactacgta gcgccggggt cgacacgggc ggccccgcga cgttcagcca
      421 ggccgatcgg cgcgcgttcg ccaacgcgct ggatcgcctg atggttgccc aggcgcgctc
      481 ggacgcctct gcccaaggat gatgacggat tgcttgtaag tcgttgattt tctgcaaagt
      541 gcacctccgg ccagccggcc actagggctt tccctcatca gaagggctgt tggctaggta
      601 tatcctcccc ggcgccccat caaaaccccg tcaggacatc aaagtgctct tctttcatcg
      661 ctcaaacagc cgccggcttg ccgcacggct cctaaccggt cttgccttga tgctcggcct
      721 ggccgctgcc agccttgccc agagcccggc aaaacccctc aaatccgtct ccatcgccct
      781 gggcacgcag gtcgtcaacg tcacctatcc ctggctgacg ctgccgatcg ccctgggcta
      841 ctggcgcgac gagggctatg acgtcaagat cgtcacggtg ggcggttccc tccagggtat
      901 ccagcaaatg gtggcgggcg gcgtccagtt cgcggagctc aactcgaccg gcttgatcca
      961 ggccaacacg gacaacgatg tcgccgtgcg cgggatcatg ggcacgggca tcatcgattg
     1021 gggcgtcggc gtgctggata acggtccgat caagacggtc gcggatctca agggcaagaa
     1081 gatcggcatc ttcagcctgg gtaccggggg catgccgctg ctcaagggct atttgcgttc
     1141 caacgacatc aatccggaca ccgacgtgca gatcatcgcc accggggccg gcgcgccggc
     1201 gctggaagcg ctgcgcgccg accgggtgca ggccttgatg ttctggggat cggcgctgac
     1261 gggattcgag aacgcgggca ccaagctgcg cgtgctggcc gatccggcct ggcgcaagat
     1321 gccggacttc acgttcggcg ccttgcaaaa aaccatcgac agcgatccga aaatggtcga
     1381 ggcgatcgcc cgcggcgcgg cgaaggcaac gctgtacgcg tggaccaatc cggaatgcgt
     1441 gcgccagatt cactggaaga acttcccgag caccaagccg accggcgccg acgaggcaac
     1501 gctggccaag tgggatctgg ccttgctgca agcccagctc gacagcatga aggccgcgca
     1561 cgaaatgaac ggcggcaagc tgatcggcgc gatggatccc gcggcgtatg gccgcatgca
     1621 ggacttcatg cgcgaacagg gaatgatcaa gcgcacggtt cccatcgagt cggtgctgga
     1681 cctgaagccc ggcttcatgg aggccgtcaa cgattttgac cacaacgccg tcatcgaggc
     1741 ggccaaggcc tgccagggcc tatgaacatc gcatcacctg cgcaaacacc cgcgttccgg
     1801 ttcgtggcag tgaacaagac ctttcgttcc aaggacggcg gcgatgtgat tgcgctgcgc
     1861 gacatcagct tcgaggtcgc gcgaggcgaa tttgtgacgg tcgtggggcc gagcggatgc
     1921 ggcaagagca ccttgctccg catcctggcc gggctggacc gggccagcgc gggcggcgtc
     1981 gagctcgcgg gcaagcccct ggccggcccg agccgcgaca tcggcgtcgt gttccaggcc
     2041 cccgtcctgc tgccctggcg caacgtcctc gacaatgtgc tggtgccggc ccagattcag
     2101 ggccgcgacg cgaagaccgc gcgcgaacgc gcgcggcact acctggaact ggtggggctg
     2161 gcgggattcg agaccaagta ccccggcgaa ttgtccggcg gcatgcagca gcgggtcggc
     2221 atcgcgcgcg ccttggtcaa tgacccgatg ctgctgctga tggacgagcc gttcggcgcg
     2281 ctcgacgcga tgacccggga gacgatgaac atggagctgc tcaagctgcg cgagcggacg
     2341 ggcgccacca tcatgctggt gacgcacagc attcccgagg cggtgctcct gggcgatcgc
     2401 gtcgtggtga tgtcgccccg tcccggacgc atcacgcaga tcgtggaggt cgaattgccc
     2461 gcccggcgca cgctcgacct catcaacacc gcacatttcg gcgggcacgt gtcgagcatc
     2521 cgcgccgact tgcatgccag cgggggaatc gattgatgag cacggtcaac ccgggccatt
     2581 cggcaatgga tcaatggctg acaccgctgc gcaggcgcgt tctggtgacg gtcttcttcg
     2641 tggccatgat cgcggcatgg catgtcgccg tgaccgcgtt tcacgtgtcg ccgcttatct
     2701 ttccaggccc cgccggggtg atccgcgccc tttacgatgg gctggtttcc ggcgagatca
     2761 tccaccatct gtgggtcacg ctttacgaaa tcctggctgg gttcgccgtc ggcagcctgg
     2821 ttggattcgt cctgggcgcg ctgatcgggc agagcgcgct gctcgaggtc gtgctctacc
     2881 cctatgtggt ggcgttccag gcggtgccca aggtcgcggt ggcgccgctg ttcgtgctgt
     2941 ggttcggctt cgatacgagc tcgaaggtgc tgatcaccgc caccatcgtg ttcttcccgg
     3001 ttgtcgccaa caccatcgtc ggcctgcggt ccgcgccgcg cgaccagatc gacctgatgc
     3061 gcgcgttcac cgcctcgccg tggcatgtgt tcaggatggt ccggctgccc catgccctgc
     3121 cgtacgtgtt cgccggcctg gatatcggca tcgtgctggc ggtcatcggc gccatcgtcg
     3181 gcgagttcgt cggcgcgcag gccgggctgg gctatttgat cctgcagcgc aatttctcga
     3241 tggatgccgc cggcatgttc gccattctga tcgtcctggc gctgatcggc attgcactac
     3301 actcgatcat ccacgcgatc gctcagcgcc tggtcttctg ggccgacgcc ttttccgatt
     3361 tgtctcgcgg aacctgacgt gtcttcccat tcgatctcca ccaaggagca cccatgaccc
     3421 agtctgaata caccctggat gcgccgcgcc gcgcctccgc cgacgaactg ccgatcctcg
     3481 atctgggaga ctggctcgcc ggcggtccca tcgagcccct ggtcgagcag ttcaagtcgg
     3541 catgcaccaa tatgggcttc ttctatgtga agaaccacgg cattccccag tccgtcatcg
     3601 acggcgtatt cgaggcgacg cgccgctatt tctcgttccc ggaagaagag cggctcaagg
     3661 acaagatcga cgagcggttc cgccgcggct tcatgccgat gggcgtcacc cagcacccgg
     3721 gccacaagcc cgacctgaag gaaagctacg aggtgggcct cgacctgccc attacggatc
     3781 ccgatgtgca ggccggccgt ccgctgcacg gccccaaccg ctggcccgcc gacaagccct
     3841 ggttccggcc ggcggcaatg gactacttcg accacacgat ggcattgggc atgaagctgc
     3901 tgcgcgtctt cgcggccgcg ctggcgcagc cggaggactt tttcctgcag tacacgaaaa
     3961 agccgatggt gcagtcgcgc ctgttccact acccgccgca aacgtcgccc accaatctcg
     4021 aactcggggc ggcgacgcat accgattacg gcatgttgac gctgctggtc caggacccca
     4081 tcgggggcct ggagcttcgc acccgcgacg gcgaatgggt cgcggcgccg tacgtggagg
     4141 gcacgctggt cgtcaacctg ggcgatctgg tcaaggtctg gaccaacgac cgctatgtct
     4201 cgaaccccca tcgggtcgcc aaccggacag gtaaagaacg gtattcgatt ccgatgttct
     4261 tcaacctcga ctacgacact ccggtagcct gcttgccgca gttccagtcg gccgacaatc
     4321 cgcccaagca tgcgccgatc aagtcgggcg actacctggt caagcgtttc gcggccgtgc
     4381 agaagtacaa gacgcccgcg gaactcgccg ccgcgtatta acggcgcctg gctgatcaag
     4441 ccccgccggc caacctgccc gttggccgca acaatccgtt ggaagttttc aggcgggcgg
     4501 ccgtgttgtc caggtgcgcg cgggccgtgg cgcgggccct gtccggatcg ccctgctgga
     4561 tggcttcata gatggcctgg tgctcggctt gcgcatgctc gcctcgggcg gcttttgccg
     4621 agcgcgagcg ggactccatc aggttcgctt tatagagctc cgtaaagaac tcgaaaaacc
     4681 ggatgtagtt gatgttgcgc gtcgcttcgg cgatggcgcg gtgaaacgcg aagtccgcgt
     4741 cgacgccgct ggcgacatcg ccggcatcca aggcccgccg catgtccagc aagccttggt
     4801 tcatcgcgtc gaggtccgcc tgatcccggc gctgcgcggc gagcccggcg gcctccacct
     4861 cgaagcctgt ccgcaattcg accgtcgcca tcaactcatc gatgtcgtcg tggtccgcgg
     4921 catgcaggcg caaaaccgaa gagcgccggt tgtcgagcac aaccagcccc acgccttgct
     4981 tgctgttgac caggccatcg accttcagcc gcgaaaccgc ctctcttagc accgtgcggg
     5041 atacgcccag gcgttccgcg atcaggtttt ccgggggcag cacgtcgccg gccgaaaacc
     5101 cgccgccagt gatctgcgtc cgcaatagcg cctcgaccct gtcggtcaag gtgccgttct
     5161 cctgtcccga tttcagcatg cccagcctcg gctaattatt ggttagttca tattataagt
     5221 tgccccgatt cattcagttt tctttcattt tagcgatgaa acgggatttc ccttatggaa
     5281 tcaatctttg taaatacgat aatcatattt gtagaaacca ataacgacac tgtccgtacc
     5341 tctcaccatg caaggggcaa gccatgaggc cgatagagga actggtccgc gccgaccagg
     5401 gtttggtcag cagggaaatc tttaccagcg aagcgctttt ccagtacgag ctgaaataca
     5461 tttttcggcg cgcatggctc atggtcgggc acgaaagcct cattcccaag cccgacgact
     5521 actttgtctc gcgcatgggc aaggactcgg tcatcgtcac ccgcgaccgg gtcgggcaga
     5581 tccatgtgct gctcaatagc tgcgcgcacc gcggcatgaa attgtgccgc tacgacagcg
     5641 gcaacaaccg ggcattttcc tgcccctacc acggctggag ttactccacc gatggcaagc
     5701 tggtcgaggc gcccgggggg ctggtgggcg tgccgggcta cgacacctac tacaagaaag
     5761 aactcgacaa gaatgcctgg ggcttgaagc gggtcccgag cgtgcacaac tacaaaggca
     5821 ccatctgggc cagctgggac cccgatgcgc cgccgctcct ggactacctc ggcggcatga
     5881 agatctacct ggattcgctg ctcgaccacc gtgacggacg ggagggcggc tccgaggtgg
     5941 ttggcggcat ccagaagtgg cgcgtcggct gcaactggaa attcccgcct gagaacttca
     6001 tcggcgacaa gcaccacgac atcagccacc gctcggccga cctggtgggc gtggggccca
     6061 gccctggcaa gggccggcgc gacctgacct ccaagcgcat gtgcgtcgcc tttcccgatt
     6121 tgggacacgg cgccattgcc cgcttgccgc acttcgaaga attcgactac gtcccgcaat
     6181 acaagggggt gccggttctc gaggaatatt tcgagcaggt ccaccaggat cgcgtgcgca
     6241 acctcggcca caagatgcgt gtcacgccgc acgccacggc gaccgtcttt cccaatatgt
     6301 cgttccatgc gctgcagcct cgctcgatcg gcctgttcca ccccatcagc ccgaccgaga
     6361 cggaaatctg gcgcatctat ctgaccgatg ccgatgcgcc gcccgaggtc aaggcggcga
     6421 cgcatcacta caacatcagc taccacggcc ccgcgggcat ggccgagtcc gacgacatgg
     6481 agaactggtc gtgcgccacc gagtcctgca ttggaccgac ggccggtgaa atggatttca
     6541 actatcagat gggcctgggc cacgccgaaa aagtcccgca cctgaacgat gcggtgtatt
     6601 ccgacttttc cgaagaaggc gcgcgcgcct tctaccgcca atgggggcgc ttcatgcagc
     6661 ggctggactg ggacgaactg cgcccgaatg cccgcaagga ggcgcagcga tgaaccatcc
     6721 cgcaccgcaa gacgacatgc agctttggtg gcaggtgcag cagttctacg ccaccgaggc
     6781 cgcgctgctg gatgagcgca aatacgagga ctggctgggg ctgctgcatc ccgacattgc
     6841 ctacaccgtc cccatcgccc gcaatgtgcg gcgcgacagg cttgccgagg aatacacgca
     6901 cgcgggtgaa acctgctggt tcgacgaagg catcgtgctg ctcaccaagc gcgtcgccca
     6961 gctaaagacc ggcatgcact ggatcgaaga gcccacctcc cgttactgcc ggctcgtgaa
     7021 caacgtccgc gtgctgggct ttgacgaggc gagccgggcc ttcgaggccg agtcgaagtt
     7081 cctcatctat cagaaccgcc tcgaggccga agtcgccatc ctggccggca cgcgcgtcga
     7141 ccggctggtg cgcgagggct cgtcgttccg gattctcaag cgggaggttt tcctggcgca
     7201 aagcacgctg cttgccaaag cgcttgccac gtttttctaa gtcggacatt cccaaaaaac
     7261 acacaagggg ccagcagtga acacgatcca accatcttcc tgcaatacga cgcggcgcag
     7321 cctgctcatc gccgccgggc tggccggcct gggcacggcg cccggcctgc ttgccgccac
     7381 ccgcggctat cccgatcatc cggtgcgcat catcttgccc ggcccgcccg gcctggccct
     7441 ggatatcgtg gtgcgcgtcg tcgccgacgc cctcggcgcc aaatacaacc agtccttcat
     7501 tgtcgaagac cggcctggcg ccggcggcat ggtcgccatg caaatgtatg ccaacaacta
     7561 caagccggat gggtataccc tgcttgccgg cggcctgggg ccgagcgtca tgatcgcttc
     7621 gctcttcaag aacgttcccc tcgatccgta caaggctttt atcggcgtgg gccaaatggc
     7681 cgaatccgcc aatatcctcg tggtgcgcgc cgacctgccg taccagtccg tcgccgagct
     7741 cgtcgccgcg gccaaggcca ggcccaatga aatcaaggtg ggctccaacg acatcggcag
     7801 ctccatgcag atgtcatacg agcttttcgc gcagcgcacg ggcacgcaat atatctatgc
     7861 cccttaccgc ggtcccaatg aagtgatggg cggcctgctg tctgaaacca tcgatgtcgg
     7921 cttcagcagc ttcgggccgt tcgttcccac cgtcaagtcc ggcaagttgc gcgcgctggc
     7981 cgtgaccacg tcttatcgcc aggatatgct gcccgacgtg ccgaccatgc aggaagccgg
     8041 cgttcacgat tacgacgtgg gcagttggct gtgcctgcac gcgctgcccg gcacgcctgc
     8101 cgccatcatc gatcaactgg gacgggacat ggtcgagatc gtcaatcggc cggagatcgg
     8161 cgaaaaactc aaggcggccg gctatatgcc gcgcccgctc gaagcgaagg ccttccagga
     8221 aaaatgggcg aaggaaatga ctcgctggac ggcggttgcc cagaacgcgc atctcgtcaa
     8281 agactatcaa accgggactt gatccgaacg aggcataaag acatggcttt cgacaaatcc
     8341 atcaatctgc tcgacctgca gcctggtgcc cgcgtcaagg atgccgaggg cgtggtgttc
     8401 caggtcgttg acaatccggg cgacggcatg tgggtcatct gcaaggccct gcaagcgaat
     8461 ggcgccttgg ccgaagagga cagcccgttg ttcgcgggga atatcgtcga ggtgctgggc
     8521 tagcctcgac cgcccggggc gggggagagc tcggccctgc cggtgatccg gcgattcttg
     8581 cgggtatggc agtcgtagtg ccacaccacc acgtgcggca ggccggcgat gtgctgggcc
     8641 tgcgccagct cgctttcgat gtattcggtg acgctcaggt cgatcagcgc gcggcgcttg
     8701 aattcgggcg tggggatgcc ggtggaaggc cgcagcagca ggcgggacgg ggcgagaccg
     8761 tggcggccca gccaggcttc ggtgcgggcg cggtcggctt ccagccgccc ggagatgatg
     8821 aggccgccgt tgcgccagta ggggtgcggc ggcagcgccg gcaactggtc gcgcgcggcc
     8881 agcgcggcgt ccaggtcggc ggcgtacagg gtttcgggga cgtcttcgac gaacacgccg
     8941 tccaggtcga aggcggtgag ccagttggcg atgtcggtgt ggctgacgcg gtcgggcgaa
     9001 gtgggggcca gttgcgagcg ctcccagggg aacaggtagc gttcgccggc ttccagcttg
     9061 gcgccgtagt agtcgggcgc caagcggctc agcgcgtccc agcacaccac catgaggtcg
     9121 acgttggcgc cgcgctgttc cagcgcgtgc ttggtgcgtt ccagcgtgtg gcccttgccg
     9181 gatacgtctt ccgccagcag cacgcgcgcg gggcggcctg ccggcaggtg gtgcaactgc
     9241 acggtgccgg cggcgcggtc gtagtgggcc gtgccgagcg cgatgtcggt catctggctc
     9301 agcatggcgg ccaggtacag gccgccgcgc gccaggccga cgatggcatc gtagcggcag
     9361 gccatcacct cgcggcgatg gtgcagggcg atgttctcga tgtcctgata ggaaaaccag
     9421 cggcgtcgct cgccgttgag gtcgacggac gtaccgacgg ggaaaggggg cgcgggatac
     9481 atggtgggca cgcagtctgg gtatgatttg gattacgatt gctgactttc ttgccgcgtt
     9541 cttccatgca ttctagtctg cccctccgcc ctgcctgccc acctggcgcc tgcgcctgcg
     9601 gccgcgaggc actgctggcg gattccgccg gggacaagcg catcctgatg ctgaccaagg
     9661 ccgaggaaaa caagctcgtc gaacgcctgg aaagcgtggc cagcctggcc gaattgcggc
     9721 gcatgcaggt gaagatgcag gacctgctgg gcatcgtggt gcatatcgcg ccgggcccca
     9781 acgaggttcg cacggtgcgc ggtttcagca tccgcatcga agagatgccg ggcctgtgca
     9841 gcaagaccaa acagaccatt ccggcggcca tccgccgctg cctggaccag cggcccgaga
     9901 tcgcctatgc gatcctgaac gaacacgacc tgctctccgg tacctgaagc ggcgtgggcg
     9961 ttgccatgaa aaacgcggca ctgggccgcg tttttcatgg ctgccgctgc tcgctcaggc
    10021 gaacgcggcc atcttgctgg ccgagccggt atcgtcgcgc gcggtttcgg ccttggacca
    10081 ggccaggcga tgcagctgcg ccatggcgat ggggtcgtcc agcaacttga ggcgctgggg
    10141 ttccgacaag ccggcgaagt gctcgacggc caggtcgacc agcgccttgc gcgcggtcag
    10201 gttggccggc acggggcggt ggcgggcggt gcccatggcg cagaccaggg cgttggtgac
    10261 cgggtcggtg atggcgtcgc cgaaggtcgg ctcgccggcg ctgtgctcgc gcgcggcatg
    10321 ggtgtagagg tgcgtgtcct ccagttcctt gggcgggcgc atttcttcgg ggatgcggaa
    10381 gagattgtgc tggatggtct tgaggcccgg gcccaggcgg ctggaataca ccgacagcgg
    10441 aatgatcagc agccaggccg ccaggatggg caccatccac cacaggaaca ccggattgag
    10501 ccagcccacc agggtggccc aggcgatgcc cagcagggtc tgcgaataat gccggcggat
    10561 ggcctcgccc cagggcgtgt cctgcgcatc gcgatgcggc gagacccact tggccttcag
    10621 gcccaggaac gccgccatca cgaagcgggt gtggaagagc atgcgcacgg gggcgagcag
    10681 catcgagaac agcacttcga tcagcatgct ctgcagcgcc ttgatgcggc cgccgtagtc
    10741 cgccggatcg cggaaccaga cgcgcagcag gcccagcacc ttgggcagga agagcagcac
    10801 gaaagtggtc gagaagagcg cgatggcctt gtccgggtgc cattgcggcc agatcgggaa
    10861 gagctggcgc ggttcgacga agtactgcgg ctcggtgagc gtgtgcaccg ccaggagcgc
    10921 cgtggacaac gccaggaaca ggaaccacag cggcgccgac aggtaggaca tcacgccggt
    10981 gaggaacacc acgcggtgca ccgggtggaa gccgtcgatg aggaacagcc ggaagttcat
    11041 caggttgccg tggcaccagc gctggtcgcg ctgcagttcg tcgagcaggt tgggcggcag
    11101 ctcttcgtag ctgcccggca tgtcgtaggc gatccagacg ccccagccgg cgcggcgcat
    11161 cagcgcggcc tcgacgaagt cgtgcgagag gatggcgccg gcgaaggcgc ccttgccggg
    11221 caggggcgcc aggatgcagt gctccatgaa gggctgcagg cggatgatgg cgttgtgccc
    11281 ccagtaatgc gactcgccca gctgccagaa gtgcatgccg gccgtgaaca gcgggccgta
    11341 cacgcgcgtg gcgaactgct ggacgcgggt gtagagcgtg tccatgccgc tggcctgcgg
    11401 cgcgctctgg atgatgcccg cgtcggggtg ggcttccatc agctgcacca gcgatttcag
    11461 gcaggtgccg gtcatgacgc tgtcggcgtc gagcaccacc atgtattggt agtgcgcgcc
    11521 ccagcggcgg cagaagtcgt cgatattgcc gctctttctc ttgacgcggc ggcggcggcg
    11581 gcgatagaag atgcggccga agccgccgac cgccttgcag agctccagcc agtgcttctg
    11641 ctcttcgatg cagatgtcgg cctggtagct gtcggagagc acgaagacgt cgaagttctt
    11701 gagcgagtcg gtgcgggcga tcgattcgta ggtggcgcgc agcccggcga agacgcgcgc
    11761 gacgtcttcg ttgcagatgg gcatgaccag ggcgcagcgc gcgccttcgg gcaaggggtc
    11821 gttggacagg ccgcgcgccg agacgctgta gcggtcgcgg ccgatcatga gctgcaggaa
    11881 gcccatcatg gccgtccaga agccggacga gacccagccg aagagcagcg cgaagaggat
    11941 caggatcagg acttccagca cctggccgcc ctggtacggc aggacgccct tcatgtacca
    12001 ggtggccacg gcggtttgca cccccacgcc cacgagcagc gccaggcgac gccggctgcc
    12061 ggccaggcgc caggcggaac cgtcgccatt ggcatgcagg gcggccaggg tttcgggcgg
    12121 gtcttgcggg cggtcggcgc gcaggcgtcg ccagacgcgc accagcgggt tgggcaccca
    12181 gggcacgggc gccaggggcg tgcgcacgtg cggcggcatg gcggcgattt cggcgcccga
    12241 ggcgtcgctg tgcatgacgc cggcggccag cgggccggcg ccgtagccca gcatcaggcg
    12301 ctggccttcc gaagccatgg agggctcggt gaccgtggtg tgctggccga tggcctcgtg
    12361 cagggccatc cagggcgctt cgccccggga aacggcctgt tggaccgagc gttccagcgt
    12421 ctggcgttgc gcctcggtca ggggcaggcg ctggatatag tcgtggatta ggccttcagg
    12481 ctcacttggc atcgggttca ttgccaggca tttggtagga ccaggtttcc gacaacggaa
    12541 tttggccgtt gctgagcgtg gcgcgcattt ccaccggctt ggtggggtcc ttgacgttga
    12601 cgcgcagggt caggcggcgg ccgccggtga tgggattggg gcgcaccgtg ttctcgacga
    12661 tctggccgtt ggcatcggcc gagacgtcgg cgctgatcac catgtcggcc ggcaggttct
    12721 tgagcgagga gccgacgaaa tcgatgatga aggccgtgct gccatcgccg tggcggatca
    12781 ggtcggggcc cttgacctcg tcgcgcgaac ggcgcgtctg catgacccag gccagcggcg
    12841 tggtgtgcgt gcgcggttcg tccagcgtcc aggtcatgtg gtattcgata tccagcggcg
    12901 tgccgacggc gggttggtcg tcgggcaccc aaaaggccac gatgttgtcg ttggtctcgt
    12961 cgggcgtggg gatctcgacc agttgcacgc tgcccttgcc ccagtcgccg acgggctcga
    13021 tccacaggct gggccgcttt tcgtagtggt cgtcgaggtc ttcgtaatgg tggaagtcgc
    13081 gcgtgcgctg cagcaggccg aagccgcgcg gatcctgcat gttgaaggcg ctcaccgcca
    13141 gcttgcgcgg gttgttcagc gggcgccaca gccattcgtc attgcccgtg tgcatggcca
    13201 ggccatcgga gtcgtgcatc tcgggccggt agttctgcgt gggcgagggc tggttggcgc
    13261 cgtacaggaa catgctggtc aggggcgcca gccccaggcg gccgaccttg gcgcgcaggt
    13321 agatgcgcgc cttgacgtcg accacggtgt cgatgttggg gcggatggtg aagcggtagg
    13381 cgccggtggc gcgcggcgaa tcgagcagcg cgtagatgac gagctcgcgc gcgcccttgg
    13441 cgggcttgat gatccagtat ttgcggaagg ccgggaactc ttcgccggag ggcagcgccg
    13501 tgtcgatggc caggccgcgg gccgacaggc cgtaggtctg gccgcggccg atcacgcgga
    13561 aatagctcgc gcccaggaac gaggccagtt cgtcgttggg cttgagcgcg gtgttgaccg
    13621 gatacagcac cttgaagccg gcaaagccca ggttcttgag caggttgggg tcgaccttga
    13681 gatcgccgta gttgaagtcg tccggatcga aattgatcgg atgcgaatgg ccctgctcgt
    13741 cgacctcatt gatggcgacg ggggtattga aatgcatgcc ctggtggtag aagcccaggc
    13801 ggaagggcgt gccgtcgtcg cgccagtggg tgcggtcgtc gcgcagctgg acgttctggt
    13861 agccggcgta tttcaggtcc tgcagctcgg tgggcaggtt ggaagcgggt tgcttgtagc
    13921 tttgcgcggc caggtcgcgg gcctgcttcg acacttcgaa gaaggtgaag ccggccttgc
    13981 tgtcttcctg ctcggccttg tttggcgtgg ccttggcggc aggcgcgggc ttgggttcgg
    14041 gcttggcggc ctcgggtttg gcggctccgg caggcttggc ggcatcagcc ggcttggcgg
    14101 tatcgggctt ggccgcctca ggcgcggcat cggccggctt gctttcggcc ggcttggcat
    14161 cggcgggctt gggcttgggt ttgggtttgg gcgcctcggg cttggccgcg ggttgttcgg
    14221 gttcgggagg aacctcgatg cggatgactt cggcgtgggt cgtgtcgacg acgggggccg
    14281 cgggttgcga ctgcgcgtag gacgttccgg tcagcagggt ggcaaaaacg agactgccta
    14341 gcgagatact gccgcggaag gcgcggttgg cgatggaaaa ggtcacagcg tggggccttt
    14401 tgcaaaaaac gaggacagaa gaagcaaggc ggaatttttg cacagatggc ggcaatggat
    14461 gtgccaaagc cccggctgta aacccgccgg agctatctgt cgcgttgccg gatgatactc
    14521 caggcgcttt catcgaggca tcggggatat ggggttttgt attcaaaagc atctttccgt
    14581 ggcaaaaaca ggcatcagcg cgaggcggcg gccggggccc ggcggcagac ccgccagagc
    14641 aacaggcaca gcaagccgca accgaacagt gccagtgcgg atacgtagat gggcaacaag
    14701 cggcctgcgc cgcgcaaggt agcggccagg ctgccggctg gcgtgagcgg cgcgacgatg
    14761 acgacgaagg ccagcaggac gccgccgagg gccagcagga acagcgccag ccagcccagc
    14821 tgcgggatgg ttggacgttt gggggcgggc gccgccgcct tggcggccgc gcgggccgat
    14881 gcggccgtgg ctgcgggttt tgccgaggac ttggccgcct tggtcggctt ggccgctttg
    14941 tcttcggcgg cgcgcggttt ggcaggttgt tcgtcgcgct gcgccgcggc aagctgggcg
    15001 cgccagcgcc aggctcccgg gatggcggcc aggccgccga agccggccag ggcgcccagg
    15061 atggcttcgg gagagatcag gcgcgggccg gcgagccagc cgggcgcctg gccgacggcc
    15121 cgccaggcgc ccatatagcc gtagagagcg tggccggcgc cgtacagggc ggccagggcg
    15181 gcggcgatca ccgtcagcac gatcagggcg ccagccatgt cctgggtgcg gcgctcggcg
    15241 aacaggttca tcgccagggc gatgagcttc aggcacagcc aggtggccac ggccagcagc
    15301 agggcgctga cgagtgacaa ggcgaagccg aagagaaaac ggacgagaaa cagcagcatg
    15361 ggactttctt ccgccgcgtg gaaggggcgg cgggcggcat gaccttagca catcgaggtt
    15421 gcggccatct cgtcctgcaa ccgccgcatc agggccgtgg cgggcacgcc gcgcgacagc
    15481 cggaacccct ggccggccca ctgggcggcg aaatcggcgc tgccgcgggc cttggcggcc
    15541 gcatgcagcg ccttgcccgc gtcatagacg atggggtagt cgggcaaggg cgggcagccg
    15601 ggggccgtgc ccagcgcggt gagccggttg gcgaagccgc gcgccggccg tccggaaatg
    15661 gcgcgggtca tgacggtgac gtggctgtca tcctgcgaca gcagggcttg ccggtaggcg
    15721 gcatcggcgg ccgactcggg gcaggaggcg aaggcggtgc ccagttgcgc ggcctgcgcg
    15781 cccagcgcca gggcggctgc gatgccggcg ccgtccatga tgccgccggc cgcgatcacg
    15841 ggcagcggca ggcgcgcggc cagcaggcgc gtcaacgcca ggatgcccaa ctgttcatcg
    15901 ggcccctcgg ggtcgaacag gccgcgatgc ccgccggcct cgatgccttg cgccacgacg
    15961 gcgtcgaggc cggcggcggc aatgcggcgc gcctcggcca ggctggtggc ggttgccatc
    16021 aggtagcagc cggccgcgcg caaggcctcg atgcgcggcg cctcgggcag gccgaagtga
    16081 aagctcacta cggccgggcg ctcgtcgagc agcatctgca gcatgtcctc gtcggccacg
    16141 aagctggtgt agatctcgcg cagcgcggcc ggcggcttgg cgccgaactc ggcaaagtcc
    16201 ggtgccaggt agtccagcca ggcgcgctcg cgcgccgcgt cggcctgggc cggcgcatgg
    16261 cagaacaggt tgatgttgaa ggcgccggcg gtgagggcgc gcgtctgccg gatggcctcg
    16321 cgcgccgcgg cggcatccat cgcgcccacg cccagcgaac ccaggccgcc ggcattgcag
    16381 accgcggcgg ccagggcggg cgtactcgtg ccggccatgg gggcctggat gatgggaagg
    16441 ctcaggttca ggctggcaag caggatcgaa tcggcggcgg tcttcatgat gtgggttacg
    16501 gtagcgtggt tgcgccgcgg ggtgcaagtt aggttagtct tcttacatgg ctctgcgtca
    16561 atcttctttt ttcatccgtt tcgtcatcct gggcgtgctt gtgcacgtgt atgtgggcct
    16621 gcgcctgatc accgatgcgc ccgtcgatgc ggctgccgcc gccggcggct ggctgctgct
    16681 ggtggcgtcc tgcgtgctga tcccgatggg catgctcagc cggcgctcgt cgcagcggcg
    16741 gccctggggc gacctgttcg cctgggccgg cctgctggtc atggggtttt tctcttcgct
    16801 cttcgtgttt acgctgctgc gcgacgcggt gctgctgttc gcctggatcg cgtcgctgct
    16861 gggggccagc gtgcccatgc tgcgcctgcg cgagatctcg tcgctgctgg ttccggcgct
    16921 ggccttgctg ggcagcgtgg tgggcttttt caatgcgcgc cggctggcgc gcgtggtgac
    16981 ggtgaatgtg ccggtcgccg gcttgcccga ggcgctggag aatttcacca tcgtgcagat
    17041 cagcgacgtg catgtggggc cgacgatcaa gtcggattat gtgcgcgcca tcgtcgccgc
    17101 ggtgaatgcg gaacgccctg atctgatcgc gatcaccggg gatgtggtcg acggcagcgt
    17161 ggatatgctg ctggcgcatg cgcagccttt gtcggggctg cacgcgccgc atggggtgta
    17221 tgtggtgacg ggcaaccacg agtattactc gggggcctcg gcctgggtgc gggagttccg
    17281 ccgcatgggg ctgacggtgc tgatgaacga gcatgtggtc atcgagccgg cggggcaggc
    17341 gatggtggtg gccggcatca ccgattacac ggcggggtcg ttcgacgagg cgcagcgcag
    17401 cgatcctgcc cgtgccttgg ccggcgcgcc tgccgatgcg gtggtgaagc tgctgctggc
    17461 gcatcagccg cgcagcgcga tggaggcggc gccgctgggc tatacgctgc agctgtccgg
    17521 gcacacccat ggcgggcagt ttttcccctg ggggtttttc gtgcagttgc agcagcctta
    17581 tacggcggga ctgcatcggc ttcaggggat gtgggtatat accagccgtg ggacggggta
    17641 ttgggggccg cccaagcggt tatgggcgcc ttcggagatt acgcggatac ggttggtgag
    17701 ggggtgagct ttgccggggg ggcttgactc ggttgctggt tcttgaggcg tccgtcgcgg
    17761 ggtgggaaga tcttggcgcg ctggttaaac ggggctgccc agcgcagccc cgtttaacgt
    17821 cgggcctagg cgtgcgtggg atgtatgccg gcggcctttg catgcaacgg tccgagccaa
    17881 accgggctgc gcagggcagc ctggtttggc gagcgagccg agattttcct ctcccctccc
    17941 aaccttggcc gatgaagtgc caagcacaca atccgtccca gcgatgcagg gaacacaaac
    18001 tactgcagtc agtcggggcg gggggaggcc gtgggcgcgc ctctgggccg agcgaggcgg
    18061 acgcgaaggg agcgcggatg cgcggacgag cgagccgacg cggtgtcggg cgcgctgggc
    18121 gcccgaccgg ctcagtgaag cgcccacggc cttcccccac cccgactgac gtcaatagaa
    18181 tgcgatccca atgccaatga acaatacgca acaccgccac caacgtaaaa aactaccggc
    18241 ggacagccaa cccaaacccc accgtcgcac acaacgcccc ggcaagaaaa acgctgccat
    18301 atccgaagtg gtcggccagc aggccggcca atggtcccgt cgcgccatag gcaatgtcct
    18361 ggtaggccgc gaaaccgccc atcgccgcgc cgcgcaattg cgggggcatg cggcggacga
    18421 tttccaggcc catggccgga aacaccatcg agcagcccag gcccgtcagc agggcgcccg
    18481 ccagcgccag ggcggggctg ggcgcctgcc acagcaggta ttgtccggcg gcttcgatcc
    18541 ccatcgacca gatcgccacc gggcggccgc ccagcttgtc gggcaggcgg ccaaagagga
    18601 tgcgcaccag cacgaagccc aggccgaagc agctcaggcc caggccggca tgcggccagc
    18661 ccttggccag gaagtgcagc gggatgaacg cgccgatcac cgcgaagccc acgccttgca
    18721 ggccgaccac ggtgccttcg cgccggatct gtcccagcac ctgccagaac gaagcacgct
    18781 gcccggcgtg cggcgcaatg gcgggcaggc cgagcgccac cagcaggccg acgagcggca
    18841 gggcggcgca caggagcatc agcgcggaga agccgctggc atcgagcatg gccaggccca
    18901 gcgggccgcc gacggcgaag gcgccgtaca tggccatgcc gatcatggcc agcacctggc
    18961 ccgagcgggc cgggcccatc atgtccatgc cccagctgat gatgccgacc agcgccatgc
    19021 tttcgcccag gcccagcagc aggcggccgg cgatgagcac gccgtaggcg ggccagggac
    19081 ccagcccgaa ctggtccgag gccaggcaga tcaggccggc gacgatgtag acgaccaggc
    19141 cgcgcatggc gcaggccttg ccgccgaggc ggtcggaata gttgcccgcg tagccgcggg
    19201 tgagaatggt cgagaggaag ggaatgccga cggccaggcc gctgagcgta ttgcccaggc
    19261 cgatgccgtg cgccacgtgc accgacatgg ccgacaacga cagcgccacg gcaaggtagg
    19321 aaaggaacag ggccagcgcc aggcgcagca gcgggcccat gcccgccgta tcgggcggcg
    19381 taggcgaaga aagggaggag gtagaagtca tatcgacgat cacattgtgt gcagtgaccg
    19441 gcgatctggg gagggatgca tcaatgcact tcccccgacc agatggccgg caggttgtgc
    19501 gttgattgac gtaaacgctt acgcaggggg catgctcgtg tcagcatgcg cccgaagagg
    19561 ccaaaatatt agcatggccc caccggccgt gcgggccggc gccatgagaa cggcgcggcg
    19621 gctcagggcg attgcctggt acgtatccag ccgcgtgccg agacggcgcg caggatgatg
    19681 ctgccgtaca gcaccatggc cacggcgttg acggtgaaca agcccgccag gccgccgcct
    19741 gcgcgcagca ccagccagcc tccgcccacg gccacggcca ggcgcacgaa accggccagc
    19801 agcggccaga caaggcggcg cgcgccttgc gaggcgaaat acatcgagaa acccatggcg
    19861 aaaaaaccgt agaacgggcc gacgatgtgc aggtaagtgc tgccggaggc ctccatgccg
    19921 gcatcccggc cgaacaggtg cagccaggcc agcggccaga aggcggcgag caggccgacg
    19981 atttctccca gcacgaacgc gatggccccg ccgctcaggg cggcctggcg cgcgcgctgg
    20041 ggctggttgg cgccgatgtt cgcgcccacc agggccacca tgggggcgcc caggccgaag
    20101 gcgatgggca tcagcaggta ttccaggcgg gccgaggtgc cgtagcctgc cagcgcggcc
    20161 gtgccggcat aggcgcccac cagggcggtg gtcgtggcga tcaggccgtt ggtgagcagg
    20221 gggttgagcg tggccaggcc gcccacggcc aggatctgcc gcaggagcgt cggttggaat
    20281 gcggtgggtt tcaggcgggc cggattgcgc ccgctcaggc aataccagcc cagcaccagc
    20341 gtgccgcccg cgaagtaggc cagcagcgtc caggcgccgc ccgcgatgcc cagcgccggg
    20401 atggggccca ggccgaagat caggcagggc gagaccggca ccaccaatag cgcgccgccg
    20461 cagatgaccg cgccggggac cagcatgttc cccgtgccgc gcacgacgct ggccagcgcg
    20521 ttcatgatcc acatcagcac caggccgccg aacaggacgt cggagtacgt gagcgcggca
    20581 tcgagcgcgc cgctgctggc gcccagcaag gtataaatgc gcgtcccctc gagcaggaag
    20641 acggccgtga agaccaggcc cagcgcggca ttgatgagca cggcgtgcaa caccagctgg
    20701 tcggccatgt cctggcggcc ggcgcccagc gcgcgcgaga tggccgccga aatgccgccg
    20761 cccatggcgc cctgcgacat gttctgcatc agcatcagca ggggggtgac cagcgcgacg
    20821 ccggccagcg cgctggtgcc caggtgcgag atgtaccagg tctcgaccag cgcggcgccg
    20881 gactgggcca gcagcatcag catattgggc cagcccatgc gcagcaacat gggcaggatg
    20941 ggggcgtgca gcatggactg caggcgggcg ttgcgggagg cgggcgcggg ggcggcagcg
    21001 gcaagggtca cggagaggtc ctgaatagtg gtatatgcca atatctaaat ggtggtatat
    21061 accattatcg ggaaccgagg caagcaagga ttcgatagat ggatgcgacg atcgcgcgcg
    21121 agagcgtgtg caacaactcg gcgctgcgcc gcgcggcgcg gcgcatggcg cagtggtacg
    21181 acgaggcgat ggcccccgtg ggcctgcgcg gcacgcagta cacgatcctg ttgcagatcg
    21241 accgcgcggg cggcgagccg ctggtgggca agctggccga ggctctggtg atggatttgt
    21301 cggcgctggg ccacaccttg cgcgcgttgc agcgcgaagg cctggtcgag gtcgtggtcg
    21361 atgcggcgga tcgccgcagc cggcgggccc gcctgacgca cagcggcaag gccaggctgg
    21421 ccaaggcgcg cgtgcaatgg gcgcgcgcgc agcggcgttt cgaggcggcc ttcggcgtcg
    21481 aggacgccgc gcgtttgcgc ggcgtcctcg acttcatcgc atcgccggat ttcgacgagc
    21541 gactgcgcgg ccaggcggat tagcccaggc gcaccggcac gaagatcttg tcgtctccgc
    21601 gctggatcag caaggccacc gtgcccttgg agttggccac ttccttgcgg atctggtcca
    21661 cgctgctggc gggcacgccg ttgatcgaca ggacgacgtc gtcgggctgg atgccggccc
    21721 gggcggccgg tccttcggcc tgctgcacca ccaggcccga ggtgccgact tcgctctctt
    21781 cctggggcgt gagccggcgc agggccaggc ccagcttgcc gtggccggcc tgggcgccgt
    21841 tgtcatcggc ggcgacgctc tgcttgtcca gcgagcccag ggtggcggtc agctccttgt
    21901 gcgcgccgtt gcgccagatg tccagcgaga catgctggcc gggcgaggcc atgctgatga
    21961 ccgccgacag gtcgcccgag gacacgatgt cgcggccgtc gagcttgcgg atcacgtcgc
    22021 cgggctgcag gccggcgcgc tcggcggcgc tgcctttttc cacgctggcg atcagcgcgc
    22081 ccgagggcga gtcgagcttg aacgagtcgg ccaggtcctg gttgacctcc tgcgccatca
    22141 cgcccagccg ggcatgctcg gctttgccgt gcgccaggat ctggtccttg atcttgtagg
    22201 ccacgtcgat cgggatcgcg aaggacaggc cctggtagcc gccggtcttg gtgtagatct
    22261 gcgagttgat gcccacgact tcgccgtggt cgttgaacag cgggccgccc gagttgccgg
    22321 ggttcacggc cacgtcggtc tggatgaagg gcacggtggt gtcgtcgggc agcgagcggc
    22381 ccttggcgct gacgatgccg gcggtggccg tgctctccag gccgaaaggc gagccgatgg
    22441 ccagcaccca ctcacccacc tgcaggtcgg acacgttgcc gactttcacg gtgggcaggt
    22501 tggaggcctg gatcttgatc acggccacat cggtctgcgg atcgctgccc agcaccttgg
    22561 cgcggaactc gcggcggtcg gtgagcttga cggtgacttc cttggcgtcc ttgaccacat
    22621 gcgcattggt caggatcacg ccgtcggggc tgatgatgaa gcccgagccc acgccttgca
    22681 tgggcacttc gtgcgagccg ccgccgtgcg ggcgcaccgc gccgggaaag ccgggaaatt
    22741 ggccgaagaa ctgcgcgaag gggtcgtcct cgtcgtcgct gaccttggtg gtgccggaca
    22801 cgctgacgtt gaccacggcc gggccgtatt ccttgttgat ctgcgcgaaa ttgggggcgg
    22861 ccatcatgcc gatgggggcc gtggcctggg tcggcgcggc ctgggcatgc gcggcgccgg
    22921 tataggcgga gaggccggcg ccgccgatgg cgccagcagc caacagggcc agcgtcaggc
    22981 gagcgatggc ggggcggttt ttcgacgggg ttcgcgtcgc tgggttttgc atcgaattca
    23041 tggtcggctc cttggggcct ggtggaacac gacgccatgg tgcaaccccc tgcttaggcg
    23101 aatcttaaga aacgttgcgg cccctcatcc tgaaaccggt ggaaacatca ctcgcacgcg
    23161 caggccgccg tccggcgcgt cgcccagggt gacggtggcg ccatgccggc cggccagggt
    23221 gcgcaccagc gccagcccca ggccgttgcc ggtggctccc gcgtcggcgc tgcgatagaa
    23281 gcggccgaag acgcgctcgc gttcgctggg ccggatgccg ggaccggtgt cgtccacggt
    23341 cagcgccacg gatgcgccgg attggacggc gatgcgcacc gcgccgccgt cgggcgtgta
    23401 cttgacggcg ttgtccagca gattgcgcac cagcagcacc agcgcgtcgg cctggccgtc
    23461 gacgacggcc gagtccggac cgtcgagggc gatgtcgatg cgcttggcat tggcctgcgg
    23521 caagacctgg gacacggcct cgcgcacgct ctgcaccagg tcggtcggag acaggaccag
    23581 gtgcggcgcg ccgttgtcgt ggcgcgccat ggcgagcatc tgctcgatga ggcgcgtggc
    23641 gcgcgccacg ccatcgccca ggcgggcgat ggcctgctcg cgggccgcgg cgtcggtggc
    23701 gcggcgcagg cccagcaact gcagctgcag ggcggccagc ggggtgcgca gctcatgcgc
    23761 ggcgtcaccc acgaactgct gctgcgaggc gaaggcctgg cgcacgcgtt ccagcagcag
    23821 gttcagttcc tgcaccagcg gccggatctc gtcgggcagg ccggcatcgg acacgggcga
    23881 caggtcgtcg ggctggcgcg ccgccaattg cgcgcgggtg cgcgcaacgg ggcgcagcga
    23941 gcggctcacc acccaccaga ccaccagcat cagcagcggc gccaccgcca ggatggggcc
    24001 gaccgaacgc agcgccagat tgcgcgcggt gaggctgcgc acgcgcaggt cctgggccac
    24061 ctgggtgacc tgcagcggcg tggccatggc atagacgcgg taggcggcat cgccggcccg
    24121 cacggtggaa aagcccagcg tcaccgggtc gggcagcagg cggcccgagg cggacttgta
    24181 gagctgcacg ccatcgaccg tccagatctg gatgatgaga tcgtcgaccg aggcggtcgg
    24241 tacgccgggg acgaccggcg ccggcgcgtt gttcaggcgc aggtcgccct ggctcagcga
    24301 cagcgccgtg ctgcgcagtt gcgcatcgaa gatgtcgtcg gtctgcgcca gcgtgccgcg
    24361 ataggccacg acggcctgga tcagcgccgc cgcgaaaatg gccaccagca aaaagagcag
    24421 cagccgcgcg cgcagggagg tcacggggaa cggcatcacg tatcgccggc cttgccgggc
    24481 tgcctgggga tgacgtagcc cacgccgcgc acggtctgga tgaagccgtg tcccagtttc
    24541 ttgcgcaggc cgtggatata gacctcgacg gcattgctgg cgatctcctc gtgccagctg
    24601 tagagctttt cctcgagctg ggcgcgcgaa tagatcatgc cgggccgcgt gatcaagggc
    24661 tgcagcacgg cccattcgcg ggccgtgagc gtgacgggtt cgccgtccac ggtggcttcg
    24721 tgcgtctgcg ggtcgaggcg cacgccgccg tgctcgaaca cggattcggc gtgcccggcg
    24781 ctgcgccgca ggagcgcgcg gatgcgggcc agcagctcgt cggggtcgta gggcttgacc
    24841 acatagtcgt cggcgcccgc gtccagcccg gcaatgcggt cgctgacggc atcgcgcgcg
    24901 gtggcgatca gcaccggcgt gcggtcgcgc cgcgcgcgca gttcacgcag caccgacagg
    24961 ccgtcctgtt gcggcaggcc caggtcgagc agcaccaggt cgtagacccc ggcccgcagg
    25021 gccgtggccg cgcgccggcc gtcgcgttcc cagtcgacgg catagccatc ggcgcgcagg
    25081 cagtcctgca cgctggtgcc tatcatcatg tcgtcttcga ccagcaggat gcgcatggcg
    25141 gtcttggggg cgggggcgca acgggacacc aagaataggg cattttgccg cgcgccgccg
    25201 ctgccgggcc gcgggcgggg cggggggctt tgtcaggggg cgacggtaga atcgggccca
    25261 tgctcagacc tcatcgaacc tggcggcggc ccgcggcgtt tcgcgccggg cggcgtttcg
    25321 tgcccgaccg cctggcggtc atgctgctgg ccgcggcggt cgtgggcgtg ctcggcgccc
    25381 tggccaccgt gctgtttcgc gaggcgctcg aactcctgca ggtctggctg ggcgggcatc
    25441 cggcgcaggg catggtctcg ctggcgcgcg gcctgccgct ctgggagcgc ctgctgctgc
    25501 ccgcggcggg cggcgtgctg gcgggactga tcctgcaatg ggcgcgccgc agcctgccgc
    25561 cgcagggcgc aagcgattat atggaagcca ttgccgtggg gcgcggcatc atcggcctgc
    25621 gccagaccgt ggcgcgcagc ctggcgtctt tgtgttccat cggctcgggg gcttccatcg
    25681 gccgcgaagg tcccatggtg cagctggcgg ccatgctggc ttccctgttc ggccgctggc
    25741 tggtctggcc gccggggcgc ctgcggctgc tggtggcctg cggcgcgacg gccggcctca
    25801 ccgcggtcta caacgccccg atcgccgggg ccctgttcgt ttccgaaatc gtctacggcg
    25861 ccgtgtcgtc ggtcaagctg gtgcccctgg tggtggcatc ggtggcgtcc aatatggtca
    25921 cgcgccagct gctgcactac gacgccgtct atcacctgcc ggctttcgat ttcgcttcgc
    25981 cctgggaggt gttcagctat ctgggcctgg gcgcgctggc tggcctggtg gcgccctggt
    26041 acttgaggct gctggacggc tcgcgccgtc tcttcagcca ttggatcggg ccgctctggc
    26101 tgcgcctggc ggcgggcggc ctggtcgtgg gcgctatctc cctcgcgtgg cccgaggtct
    26161 ggggcaacgg ctacagcgtg gtcaattcca ttctgcacca gccctgggcc tggcaggcgg
    26221 tggccgcgct gctggtgatg aaggtgctgg ccacggcggc cagctcgggc tcgggggccg
    26281 tgggcggcat tttcacgccc actttgttcg tgggcgccgc gctggggcac ttgtacggca
    26341 ccgccttgca cgacgcattg ccgcaggtgt cggcggtcag cagctatgcc gtggtcggca
    26401 tgggggcctt gctgtcggcg gccacgcatg cgccgctgat gtccattctg atgatcttcg
    26461 agatgacgct cagctacgag gccatgctgc cgctgatggt ggcctgcgtc atggcttatg
    26521 ccgtggtccg ggccctgggc gccaagtcca tgtatgccgg ctcgctggcc gacaaccgcg
    26581 gcgcgcggcg cagggccgac ggccactgag cgccggccgc cgcgctgcag gacttattgc
    26641 ggcgcggcgg ggatgaccgt catgcgcagt tcggagtgcg gttcttcgta gttgcgcggc
    26701 gcggccggcc ggcgcgccgc cggcttggcg ggcggggtgg tggggggctc ggccgaaggg
    26761 tccgcaacgg tgtctggcgg ctgaagcgct ggcgtagccg gcatggcggg catggtcggc
    26821 atagcgggca tggtcggcat ggccggcata gcgggcatgg acgatggcgc ggcggccggc
    26881 gcgggcgccg tcatgggggc gatgggggcg ggcggttcca ccggcgcgat ggacgtggcc
    26941 ggttgagcct gcgtggtggc ggaggtctgg ccctgggcgg tgcggctggc cgcgagctgg
    27001 tgttgcagat cgtcgatctg ggcctgcagg cgctggcgtt ccagcgtggc cgaattcagt
    27061 tcgctgcgga cttgttcgga ttgttcggtg gacagccgcg gcggcccgta gttggtttgc
    27121 aggaacagca cgccgcccgc gcccagcgcg atgccgatgc caagcagcgc cagccagcgc
    27181 ggcaagcgcc ggttgtgccg gcccggctgg tagatcgatg gcttgaagac ttgtcgccgc
    27241 gattgtccga acatgcccat gtaaaaactt cctattcctt ttatgggagg cgaaaaaatc
    27301 aaagcatact gtagcggttc gtgccaagtt cggcgtcgag cgcttgcaat cgttgtggcg
    27361 tgcccacatc gacccagcgt ccgccgtgcc ggacggcctg cacggcgccg cgcgccatgg
    27421 cctggcgcag cagcggcgcc agcggggcgg cctggccggc cacgaggccg gcaaacaggg
    27481 ccggccggta gacgccgatg ccggaaaagg tcagcctggc ctggccttcc tccgcagcgc
    27541 ggccgtccgg ctgcagggcg aagtcgccca gcggatgctg cggcgggttg ccgaccagca
    27601 gcagccaggc gtcggcgccg cttgcgtcga ggcgctgccg cgccgctcgc gcctgggccg
    27661 gatcccagtc gcaccagata tcgccgttga tcaccaggaa cggctcggcg cccagcatgg
    27721 gcagggcctg cacgatgccg ccggcggtct ccagcgcctg tccttcggcc gaataccgga
    27781 tgcgcgtgtc ccatcggctg ccgtcgccca gggcttcgac gatgcgctca cccagccagg
    27841 cgtggttgat gaccacgtcg tgcaggccgg cccgcgccag ccgctcgagg tgccagacga
    27901 tgagcggctt gccgcccacc ggcagcagcg gcttgggggt gtggtcggtg agcggccgca
    27961 tgcgctcgcc gcggcccgcc gccaggatca tcgcctgcat cagaaggtgt accccacggt
    28021 cggcgttgtc cggtcgagct ggtcgagcag gcgcagcagc ggcgcgaaga cgccgtagcg
    28081 ctgcgccacc tggcgcacgt atcgattgac gcgcggcatg tgggccagat agcccggttt
    28141 gccatcccga tggttcaggc gcgcgaacac gcccaggatg cgcagattgc gctgcaggct
    28201 catccattcg taggcgcggt ggaactcggc gaaatcggca tcgaccggca agccggcctg
    28261 gcgcgccatg tcccagtagc ggatggccca gtccagctgc tggggttctt cccaggtggt
    28321 gcgggcatcg gtgaccagcg aggccaggtc gtaggtgagc gggccggcca gggcgtcctg
    28381 gaagtcgagg atgcccgggt tgggcccgaa gcgcggttcg tcgcagacca tcaggttggg
    28441 cgaatggaag tcgcggtgca catagacggg tgtctgggcg gcgcaatggg tcgacagcag
    28501 ctggagtagt tgccgcaggt cggccgcctg cttgtcggtg agcgaggcgc cgtggtggcg
    28561 ctgcacatac cattcgggaa acagctcgag ctcggcggcc aggcgcgccg cgtcatagga
    28621 cggcaagcca gtgatggcgg cttgttgcat atgcaccagc gcggccagcg cgtcgcggta
    28681 cagggattgc agccgggcgt cgtccaggcc ggcctggatg cgctggtaat aggtttcgtt
    28741 gcccaggtcg gacagcagca gcagcccttg ttcgggggcc tgggccagca cggcgggcac
    28801 attcaggccg gcctcgcgca gcaggccgcc ggcatgcagg aaggggccca cgtcctcgtg
    28861 gggcggcggg gcgtccatga cgatggcggt gccgttgccg gtctcgatcc ggaaatagcg
    28921 gcgaaagctc gcatcgctgg aggcggcgcg cagggtgtcg gggcgcaggg ccagcccgga
    28981 tggcaggctg cgcagccagg cgtcgatcag ggcctggcgc ggatcgtgtg ggttggtcaa
    29041 gagagccgtc tcgaaatctg cgaaattgaa gaaaaacccg tcagttcgcc cgggattgtg
    29101 cagggaataa cccgcagctt gggcgtttgg ccggcttctc tataataccg gctcgcttca
    29161 gctccggcgt ccttcgaccg gtggggggcc cattgtcaaa caaggttgat gtttgtgctc
    29221 gtgcgcatgg tccggtggtt gattcttctc gcagtcggcg cagccggagc cgcgcaggct
    29281 caggccgtct ccggtacgga cgccagcccg gccgcgggcg ccgccgcgca gccggccgcc
    29341 aaccaggtcg aggtgcgggg catccagccc gcggtcgacc tgcgcagcac ctcgggcctg
    29401 cgcgtgcatc gccagactga cgacaagatc ccggccttcc tcgagggcga ccggatcgac
    29461 ggcagccccg acacccggct caccctcacc ggcaacgccc aggtgcgccg catcgacacc
    29521 gtgatcaagg gcgacagcat cgactacgac cgcaagaccg gcgaggcgac cgtcaccggc
    29581 catctgcgcc tgcagcgcga cgccacgctg gtgaccggcc cgacggggca tgtcaatgtc
    29641 gacagcgacc agggcgagat cgacaagccc gatttctgga tcggcgccac cggcggcacc
    29701 gcccgcgccg agcatgccga catcttcagc aagtcggaat tccgcctcaa gcaggtcacc
    29761 tacagcggct gctcctgcgc ccagccgtcc tggtacatca aggccgacag cctcgatgtc
    29821 gacatggacg agaacgaggg cgtggcgcac aacggcgtgc tgtacttcaa gaacgtgccc
    29881 atcctggcct cgccctacct gaccttcccg gtcaagaagg agcgcaagtc gggcttcctg
    29941 gtgcccatgt acggcatgtc cagcaatact ggcatcgaca tctcgatgcc gtactacctc
    30001 aacctggcgc ccaactacga cgtgacgctg acgccgcgca tcatgagcaa gcgcggcgac
    30061 atgctgagcg ccgatggccg ctatatcggc gacggctaca ccgggatggt gcagggctcc
    30121 ttcctgccga acgacaggct ggccgaccgc gaccgatgga tgtatcgctg gacgcactat
    30181 cagcagctgg gcaacggctt ctacgccgac tgggacctgg cccgcgtgtc ggacaacaac
    30241 tactaccgcg acatcaccac catcggcctg aattcggcca ccaccgtcta tctgccgcag
    30301 agcgcccggg tgggctggaa cggcacctac tggcagacgt cggtgcaagc catccgctac
    30361 cagacgctgc aggatcccac ctcgcccatc gcgccccagt tcgacgaact gccggccatc
    30421 aggctgaccg gccaccgcta cgactggaac ggcctggacc tgtcgatgga ctcttcggcc
    30481 atccgcttca gccggccgct gctcgagggc acgcgcctga gccccaacgg cgaccgcgtg
    30541 cagatctatc cctcggtgtc gtatcccatc gtgcggcccg gttggtacat cacgcccaag
    30601 atcggcgtga actacaccca gtacggcagc accaactggt acaacaccga cttcaacggc
    30661 ctgggcagca cggcgcccta tcaggcgtcg cagtcccgca cgctgcccat cgcctcgatc
    30721 gacagcggca tggtgttcga gcgcgacacc tcgctgttcg gccggtcggc gacgcagacg
    30781 ctggagccgc gcctgtacta cctgcgcatt ccctatcgca accagtccaa cctgccggtg
    30841 tacgacacct acctggcccc cttcagcttc gaccaggcct tccaggaaaa ccagtatgcc
    30901 ggcggctggg accgcatcaa caacgccaac cagctcaccg cggccctgac cacgcgctgg
    30961 atcgacaacg ccacgggcct ggagcgcttg tcgctgtccg cgggccagca aaagtacttc
    31021 accgaccagt tggtcaccct gccgggcgag gtggcgcgca tccaccagcg ttccgattac
    31081 ctggcgggcg ccagcgccgc gttgaccgac acgctcagca ccgagatcgg cgcgcagtac
    31141 aacccttatt ccagccaatg gtcgcaggcc tacagcagcc tgcgctggac gccgcagcgc
    31201 ctggccacgg tggcgctgac ctatcgctac cagcgcaacc cgctgcccga tgtaccatac
    31261 caacccatag gccagaacca gatcagcctg ggcgtgcaat ggccgttcgc caagcactgg
    31321 tacggtgtcg gacgcctcga ctacacgatc ggttcggaag tgtccgacag tacgtcccgt
    31381 gttccgcagg ccatcatcgg tgtcgaatac aagtcggatt gctgctggtc ggctcgcgtg
    31441 gtgttccaac gctacgcgac ctcgtcttcg caggtcaata cggcgctgtt cttccagctc
    31501 gagctcgaag gtttgggcgc gctgggcagc gaccccatga gtttgatcaa tcgcagtatc
    31561 ccgggctacc agcccgtgac gccggcgcag cagcccggca cctcctttga aaggtacgag
    31621 taatgcgtag attgtcgctg cgcggccgta gttccggctg catcctgatg ctgaccctgt
    31681 gcgtggccct gggcgcttcg cctgtccatg cgcaaaacgc aaagcccgat tcctccggcg
    31741 ctgccaagca gtcagtcaaa gccaaggcgc ccgccaagaa gcccgcggcc aagccccaac
    31801 cggctcccgc tcccgcggcc agcgccgggg gcgagcagtt cggcgacggc atcgccgccg
    31861 tggtcaacaa ggaggtcatc accatgcgtg atttgcgcga agcttcgcag cgcacggcgg
    31921 ccgacctcca gcagcacaag atccaggtgc cgcccgacga cgtcctgcag cggcaggtgc
    31981 tgcaacagct catcatggac cgcctgcagg agcaggaggc caggcgcctg ggcatcgcgc
    32041 tgaccgatgc gcaggtcgac caggcggtcg gcatgatcgc cgaccgcaac cggctcaccg
    32101 tcgaccagct caaggccgag atcgccaaga ccggcgcgag ctgggacggc tatcgcaagt
    32161 ccatccgcga agagatccgc actacccgcc tgcgccagcg catggtcgac tccaacatca
    32221 cgatcaccga tggcgaggtc gatgccttcc tgaaggagca tgcgcgcgcc gccgccgcgg
    32281 cgccggcccc ccagcccgtc caacctgctc cggctcccgc gcagcccacg gtcgcgatcg
    32341 tgcccaacgg cccgatcgcg ctggcgcaga tcctggtgcg cgtgcccgag ggcgcctcgt
    32401 ccgaggatgt cgaccgcctg cgcaagaagg ccgagggcct gctggcccgc gcccgcaagg
    32461 gagaagactt cgccagcctg gccgcggcca actccgacgg tcccgaggcc ttgcagggcg
    32521 gccagatggg cgcccgtccg atcgacggct ggcccgatct gttcgtgcag gccgtccgtg
    32581 gcttgcggcc cggccaggtc agcggcctgg tccgcagcgg caacggtttc cacatcctca
    32641 aggtgatggc ccgcgcgatg cagcagatcg ccgcgcccac gcaggcggca cccgccccgg
    32701 cggcggatga agaccccatt ccgcccgaat cttccggcgc cgtgcagatc acccagacgc
    32761 atgcccgcca catcctgatc aagctgtcgg ccgtgatgaa cgacgagcag gcccgcgaga
    32821 agctcgatga catccgtcgc cgcatcgtcg agggcggcgt ctcgttcgcg gatatggcga
    32881 gccagtattc gcaagacagc accgcgccgc aaggcggcga cctggggtgg ctcgatccgg
    32941 gcgagaccgt gcgcgccttc gaacaggtca tggacaagct gcagcccggc gagatcagcc
    33001 agcccgtggc gtcgcctttc ggctggcact tgatccaggt gctcgagcgc cgccagcatg
    33061 acgccaccga agacgtgcgc cgcaaccgtg cccgccagat cctgctcgag cgccgcgcgc
    33121 aaccggcttt cgaggattac ctcgatcagc tgcgggccca gtcctacgtg gacaaccgcc
    33181 tggaaaagca ggaccagctc cagcagcgct accagcagta gatgagacct ccacgcccac
    33241 tgcgcccggg ctcatccccc gaaggggctt cggcctcctt cgggcggccg tgcggaggct
    33301 gacccatggc ccagcatcag gcccgcaagc gcttcggcca gaatttcctg accgatgaga
    33361 gcgtggtgga cggcatcgtg cgggccatcg cgcccgccgc cggcgatcac gtcatcgaga
    33421 tcggcccggg cctgtccgcg ctgacgcggc cgctgctgga tcgtttgccc agcctgacgg
    33481 tcgtcgagat cgaccgcgac ctggccgcgc ggctgcgcaa gcagtacccg gccgagcggc
    33541 tcaccgtgat cgaggccgac gtgctcaccg tggatttcac caccctgggc gaaggcctgc
    33601 gcgtcgtggg caacctgccc tacaatatct ccagcccgct gctgtttcac ttgatggcgg
    33661 cggccggcca ggtgcgcgat cagcatttca tgctgcagcg ggaggtcatc gaccgcatgg
    33721 tggccaagcc cggcaatgcc gatcacagcc gcctgtcggt gatgctgcag tcgcgctatc
    33781 gcatgaccaa gttgttcgac gtgccgcccg aggccttcga tccgccgccg aaggtggtgt
    33841 ccgccgtggt gcgcatggtg ccgctgggcg aagatcgccc gcggccgcgc agcgatgcgg
    33901 cattcgagca ggtggtggcc aaagcgtttt cccagcggcg caagatgctg cgcggcgtgc
    33961 tgggcgactg gaccgcgcat atcccctggg agacgctggg gatcgagccc acggcgcgcg
    34021 cggaagcggt gtcggtggtt gggttcatcg gcatggcgga tgccttggtg gctgcgggcg
    34081 tgctgccggg ttcttgaggc gcattgcccc tcggcggcaa tgctcagatc atcggctcgc
    34141 gatgcgttcg gcgtgcgcgt agatcgtggc gtcttcgccg cgcatgaatc ccagcagcgt
    34201 caggttggcc tgttgcgcca ggcgcaccgc cagggcggtc ggcgccgaga cggcggccag
    34261 gatgcccgtg ccgcaggagg cggccttctg caccatttcg aagctggccc ggctggacac
    34321 cagcgccatg ccttgcgcgg gatcccggcc ggcgcgcgcc agggcgccga tgagcttgtc
    34381 cagcgcgttg tggcggccca cgtcctcgcg caccagctcc atctggccgt ccaggcgggc
    34441 ccagccggcg gcatgggtgg cgccggtggc gtcgtgcagc acctggcgcg cgcgcatggc
    34501 ccgcatggcc tgcagcacgg ccgccaggtc cgcgggcgcg ccggccgcca ccggggcgac
    34561 cggccgcaat acctcgggca gggtttccac gccgcacagg ccgcagccgg tgcggcccgc
    34621 catggcgcgg cgccgttcgc gcagccgcac cgcgcaggcg ctggctattt ccagctccac
    34681 cacgatgccg tcgcattcct ggcgcaggtc gatgccgcgg acgtcggaca cgccgtcgac
    34741 gatgccttcg gtcagggaaa agcccagggc gaagtcttcc aggtcggccg gcgtggccag
    34801 catggtggcg tggctgatgc cgttgtattc cagggcgatg ggggtttcct cggccagcgc
    34861 gtcgtcgcgc gggttggcct cgatccggcc gccgcgcacc cggcgcacgg ctacccgcgc
    34921 gctggcgggc gggtcgtgtt cgtcggaaac gggaaagggc ggcatgacgg cgggcagggg
    34981 gcctgatcgg ggtgattcta gtgtacgccg cgggccgggc cgcccgcctg catgaagcgg
    35041 gcaggcgcga tatgatgttt cgatgtccaa agtgattttc ctcgcggatc ggcgtgccgc
    35101 ccagcccatg gtccatgagg ctgcgagcct tgcatggcaa ccgggggaac ctttgctgga
    35161 cgcgtttgcc aggcccatgc gcgacctgcg catctcggtc accgaccgct gcaacttccg
    35221 ctgcacctat tgcatgccgc gcgaggtctt cgacggcagc tacccgttca tgccgcattc
    35281 ggacttgctt tcgttcgagg aaatcacgcg cctggccggc ctcttcgtgc agatgggcgt
    35341 cgagaagatc cgcctcacgg gcggcgagcc gctgctgcgc aaggacgtcg aatccctgat
    35401 cgccatgctg gccgggctgc gcacgccgca gggccggccg ctggacctca cgctcacgac
    35461 caacggcgtg ctgctggcgc gcaaggcccg cgcgctgaaa gacgccgggc tcacccgcgt
    35521 cacggtcagc ctcgacgcgc tcgatggcga catgttcgcg cgcatgagcg acagccgctt
    35581 cacgcccgac gacgtgctgc gcggcatcga tgcggccgcg caagccgggc tggcgccgat
    35641 caaggtcaat atggtggtgc gccggggtgt caacgacggg cagatcctgc ccatggcgcg
    35701 ccgctttcgc ggcacgggcc acgtgctgcg cttcattgaa tacatggatg tcggcagcac
    35761 caacggctgg aacctcgccg aagtgctgcc cagcagcgag gtgctggccc gcatcgccgg
    35821 cgaatttccg ctgcaggccc tggaaggcgc cgccatgggc cgggtggcgc agcgctggcg
    35881 ctatgccgac ggcgcgggcg agatcggcgt catctccagc gtgtcccatg ccttttgcgg
    35941 cggatgcacg cgcgcgcgcc tgtcgcccga aggcaagctg ttcctgtgcc tgttcgccag
    36001 ccagggatac gatctgcgcg agccgctgcg ccacggcgcc agcgatgcgc agctggcgtc
    36061 ccgcctggcc ggcatctggt ccggccgcag cgacaattat tccgaacgcc gcgcccagtc
    36121 cacgcccgag caccaggccg cgcgcaagat cgaaatgagc tacatcggcg gctgaaagat
    36181 gacgaccccc atgtccaatc cttggggcgg cccggcggcg ctggcatgct tggcgtcggc
    36241 ggcctgatcc tggcgggcgg gcgcggctcc cgcttgcggg aggccgacaa gggctggctg
    36301 atgctggccg gcctgccctt ggtggaccac gtggcgcagc gcctcgcacc ccaggtcgac
    36361 acgctgctga tcagcgccaa ccgcaacccg gaacgctatg cgcagtacgg ccaggtgctg
    36421 gccgacgacc cgctcttcgg cgcctggtcc ggcccgctgg ccggcgtggc ggcgggcttg
    36481 cgggcctggt ccggcccctg gctggtcgtg gcgccgtgcg acacgccgtt cctgccgcgg
    36541 gacatggcgg cgcgcctgat cgacgcggcc caggcgcgcg gggcgccgct ggccgtggcg
    36601 cgcgcggccg gccggcggca ctcggtctgc atggcgctgc gcacctcgct cgcggacgac
    36661 ctgcgcgact atctgcaagg cggcgaccgc aaggtggagc actggcagac gcgcgtgggc
    36721 ggcgtcgagg ccgacttcga cgacgcgccg cacgccttct tcaacatcaa tacccctgaa
    36781 gatttagcgg ccgccgcact ggacggaaac ggccggcagt aatatcggcg gatcgacacg
    36841 tttttcgtga gagaccgcca tgacccgccg tacccccatc gaacgctacc gcaacatcgg
    36901 tatctctgcc cacatcgacg cgggcaagac caccacgacc gagcgcatcc tcttctacac
    36961 cggcattacc cacaagatcg gtgaggtgca cgacggcgcc gcggtgatgg actggatgga
    37021 gcaggagcag gagcgcggca tcaccatcac ctcggcggcc accaccgcct tctggcgcgg
    37081 catggcgggc aattatcccg agcaccgcat caacatcatc gacaccccgg ggcacgtcga
    37141 cttcaccatc gaggtggagc gctccatgcg cgtgctcgac ggcgcctgca tggtctacga
    37201 ctcggtgggc ggcgtgcagc cgcaatccga aaccgtctgg cgccaggcca ataagtacgg
    37261 cgtgccgcgc atcgccttcg tcaacaagat ggaccgcgtc ggcgccgact tcttccgcgt
    37321 gcagcgccag atcggcgagc gcctgcgcgg cgacgccgtg cccgtgcaga tccccgtcgg
    37381 cgccgaggac aacttcgagg gcgtgatcga cctggtcaag atgaaggcca tcatctggga
    37441 cgacgccagc cagggcgtgc gcttcgaata ccaggacatc cccgccaacc tgcaagtgca
    37501 ggcccagcag tggcacgaca atatggtcga gaaggccgcc gagggcaacg aggccctgct
    37561 cgacaagtac ctgtcgggcg agccgctgac ggaagacgag atcaagcttg gcctgcgcct
    37621 gcgcaccatc gccaacgaga tcgtgcccat gctgtgcggc agcgccttca agaacaaggg
    37681 cgtgcaggcc atgctcgacg ccgtcatcga ctacctgcct tcgccgctgg acgtgcccgc
    37741 catcaagggc cacagcgtcg acgacaagga gatcgaacgc catgccagcg acaccgagcc
    37801 tttctcggcg ctggccttca agatcatgac cgaccccttc gtcggccaac tggtgttctt
    37861 tcgcgtctac tcgggcatgg tcaagtcggg cgacacgatc ttcaacccca tcaagaacaa
    37921 gaaagagcgc ctgggccgca tcctgcagat gcacgccaac gcgcgccagg agatcagcga
    37981 ggtctacgcc ggcgacatcg ccgccgccgt gggcctgaaa gacattacca ccggcgagac
    38041 gctgtccgat ccggcgcacg tcatcatcct cgagcgcatg gtcttccccg agcccgtcat
    38101 ctcgcaggcg gtcgagccca ggaccaaggc cgaccaggaa aaaatgggcc tggcgctcag
    38161 ccgcctggcg caggaagacc cgtcgttccg cgtgcgtacc gacgaggaat ccggccagac
    38221 catcatctcc ggcatgggcg agctgcacct ggagatcctg gtcgaccgca tgaagcgcga
    38281 gttcaacgtc gaggccaatg tcggcaagcc gcaggtggct tatcgcgaaa ccatccgcgc
    38341 cacggtcgac gaggtcgagg gcaagttcat caagcagtcg ggcgggcgcg gccagtacgg
    38401 ccacgcggtg ctcaaggtcg agccgcaggc gcccggcaag ggcttcgagt tcgtcgacgc
    38461 catcaagggc ggcgtggtgc cgcgcgagtt catccccgcg gtcgaacgcg gcatccgcga
    38521 cagcctcaac gccggcgtgc tggcgggcta tccggtggtc gacatcaagg ccacgctggt
    38581 gttcggctcc tatcacgacg tcgactccaa cgagaacgcc ttccgcatgg cgggcagcat
    38641 ggccttcaag gagggcatgc gccgcgccaa gccggtgctg ctcgaaccca tgatgcacgt
    38701 cgaggtcgag acgcccgagg actacaccgg ccccgtcatg ggcgacctgt cgtcgcggcg
    38761 cggcatggtg cagggcatgg aagacatcgc tggcggcggc ggcaagctgg tgcgcgccga
    38821 ggttcccctg gccgagatgt tcggctattc gacctcgctg cgctcactga cgcaggggcg
    38881 cgccacctac accatggagt tcaagcacta cgccgaggcg cctcgccagg tggccgagga
    38941 agtcatcgcg gcgcacaagt cctaggccgg ctcattccag gaatgcctcg atcgccgcgt
    39001 tgaaggccct ggggttggac aggttcatgc catgcgacgc gtgttcgatc gacacgcgct
    39061 gcgttgcggg cagcaggcgt tccagccagt cgatcacggc cgggaagggg gcggggctga
    39121 gcgcgccgcc gatcagcagc acgggcggca gcagcgcccg caaggcgtgc tcgtcgggca
    39181 ggggaaggat ctcgtcggcc tggccgagca gggtggcggc gttgtcgcgc accatctcct
    39241 tgaaccaggg caccatgcgg cgccaggtat ccgggccggt cacggcgtcg atgaagagcg
    39301 ccaggccctc ttcggcctgg ccggcgcgga tcaggtcgcg ggcgcgctgg cggaagttgc
    39361 cgcgctgatc ggcatgtccg ggcagtcgct ggccggggtc ggccagcgtc aggctgcggg
    39421 ccaggccggg gttgcgcagg gcggcctcca gcacgacgcg gccgccgcgg gagtggccca
    39481 ccaggtgggc ggagccgccc gcgacctgct ccacgaactt cagcaactgg tcggcgtgtt
    39541 cggcgatcga gaagccgtcg ccctcgccgt tccagcgctc gggccagtag cgccgcagcg
    39601 acagcgccag cacgcgaaaa tgcctgccca gcccggcgat ctgcggcttc cagtagcgcc
    39661 agtcgcagag cgacccgtgc accagcacca gtggcgtccc cgcgccgtgc tcggcataag
    39721 cggtggtgta gccgtcgaag tcgacgctcg aggcaggcgg gtcgggagca aaacgcatgg
    39781 cgggggcagg tctggacaag cccatgattc tagcgccgcc gcccggcacg ccccggtggc
    39841 ggccccggct tgctaaactc gctgcgccat ggggctggtg cggcgccgtc gtgccgactc
    39901 gtcaggcagc cctcagtcag ctagatttct ttgcaattta ttgtccgaaa ccatgtccgc
    39961 cgagcccaat tccctgcccg aatccgccca acgtgttgcg ttattgctgc agtcgctggg
    40021 ccacgacaaa cccgtcgtcc tgctggagca ggccggccgc acgtcggcgc aagccgccgc
    40081 gggcctgggt tgcgaggtgg cgcagatcgc caagtccatc atctttcgcc gcgtgtccga
    40141 caacgcgccg gtgctggtgg tggccagcgg catcaatcgc gtggacgaag ccaaggtggc
    40201 tgcgcgcgtg ggagcgctgg gccgcgccga cgcgcgtttc gtgcgcgagg ccacgggcta
    40261 cgcgataggc ggcgtatgcc ccatcggcca tgccatcaag cccgtgacct tgctcgacga
    40321 agatctgttt cgctacgaca gcctgtgggc ggcggcaggc cacccgcatg ccgtgttcaa
    40381 cctcacgccg cggcagctcg aagccatgac gggcgcgccc gtggccgatg tggccttgcg
    40441 cgcatagcgt caagcggaac gcgcgacgag cgatggttcc gcgtccgttg cgccggacga
    40501 ggccacgacg ttgtgcacaa agcgcagctt ctggacgacg gcgggcgcca gcacgaaggg
    40561 gtatgcgtcc ggcatgccca tgctgcggtt caggctgttc agcgcatagg tcaacggaaa
    40621 ccagcggcgt atcaggttgc cgaagctcgc ttcgtcgacg gaagtctggt cggacagcgt
    40681 cggcaggctc tcgtcgtccg gggcgaggac gagtccgcac gccgtcgcgg tgtcgagcgt
    40741 atcgacgatg tgcaggtagt gcgcccacgt ctccgcccag tcctcccatg ggtgcatcgt
    40801 cgcgtattcg ctgacgtatt cgtgctccca gcccgccggt gcgccattcg cgtagtgcga
    40861 gtcgagcgcg gcctggtagt cggcgcgctc gtcgccgaac tgggcgcgga acggctcgag
    40921 ccaccgggtg ccggccacga gccggtcgaa gtagtaatga cctatctcgt gccggaaatg
    40981 gccgagcagc gtgcgatacg gctcatgcat gtccgcgcgg atccgttcgc gcgtcgcatc
    41041 gtcggcttcc gcgagattca tggtgatcag cccgtccgcg tgtcccgtca tcacgggttc
    41101 cgcgtccgtc gactccttga acgcaaaact catcccgcgc tccgggtcct gcgcgcgcga
    41161 ttcgaccggc agcccgagca cgagcagcgt atacaacagc cggcgcttcg cggcttcgag
    41221 ccggtaccac agcaagcggt tgtcgggcga cgacaggttc ggaatcgttt cggtcagcgc
    41281 gcacgcctgg cacagcgcgt cgctgctgtc cgcggggatc atccagttgc agacgttttc
    41341 gacggcgtaa ttgccgcatt ggcggtagag gcggcccgcg gcctgcggat gcagggcgcg
    41401 ccatgcctgc ccgcccgcgg gttcgaacgc gccgacttcg ccgacatccg gaagatagcc
    41461 gagcagcgag ttgcagcgct cgcatttgac gttttcgaag aaaaccaact gtgcgcactg
    41521 gccgcagtgg aaagttttca tggcgtatcg tcctcgttct gaactgcggc ccttgtcgtc
    41581 gcgcgcgggc aggccctatt gcagcagttg cagccggctc ttggccgtgc tggcagcctg
    41641 cgtgctggga tactggctga tcagcttttg cagcgtggcc ttggccgcag cgcggttgtt
    41701 cagctcgatc tggctgccgg cgatcaccag cagggcgtcg ggcgcgcgcg cgtcgttggg
    41761 cgcgtcctgc accaggctgt tgagctgcgt gatcgcgccc ttgaagtcct tgagcgcgta
    41821 gcgactgctg ccttggtaga agcgggccga cggcgccagg tcgctgttgg ggtagagggc
    41881 gacgaaggcg gacagcgagt ccgacgactc tttgtactgg cccttgcgga acaggtcgat
    41941 ggcgttgttg taggtggcgg cttcctgcgg gtcggcaacc gggccgccag gagcggtcgc
    42001 ggcgccttgc tgggcattgc cggcggcctg ttgctgcggt tgcggacgcg acagttcttc
    42061 gatctgtccg cgcaattggg cgagctgctg ctgcagcgct tgaatctggt cggacagttg
    42121 cagctgggcg cgctggctct gttcgaattg ctggcgcagt tcgagaatgg cacggcgcgc
    42181 gtcatcatcg gcaaatgcat aggccggtgt cgtcgcggcg gcgcaagcca ggctggcgat
    42241 cagcgccagt cggcgcaaag ttgttgctcg gtctcgcatg ttatttccac gtgacagaaa
    42301 agcaaacgtc gggaccgggt cccgacgtgg atattcaaag tcggaactgc cggtctatgt
    42361 cggcaggccg attccatcca tgcttagcgg atgtagtcga tgtcggcacg gcggttttgc
    42421 gcccaatcgg actcttcgtg gccgagggcc ttgggctttt ccttgccgaa gctgatcgtt
    42481 tcgatttggc tgtcgctcac gcccagcagc gtcatttggc gacgaacggc ttcggcgcgg
    42541 cgttggccca gagccaggtt gtattcggag ccaccgcgtt cgtcggtgtt gccttcaatc
    42601 ttgatgtgtt gttgctggtg accggccagg tagcgggcat gggtttcaac caggccgcgg
    42661 tattgttcgg cgacgctgta gctgtcaaag tcgaagtaga ccgagcgttg ctgagccagc
    42721 acgctgttcg gattgaacgg gtccatggga tcgccagagg tgccaccgcc agcgccgccg
    42781 ccggcaccgg cgttcttgtc gaggggaacg gagctacagg ccgccaaagc ggcggtcaga
    42841 gcagcgaggg aaaggctttt ggcaatgcgc gacttcatga tggttccttt attgagagaa
    42901 gagcacacgt gttatcgggt aaatgggccc cacgttggtt cacgtatttc cccgttcaag
    42961 acagacagcg tctgacgtac gcggccgtcg ctcgatacgc ctgccagtac actccgcccg
    43021 ccttggacgg tagcataaag cacctgcatg ccattcggtg caaagcttgg tgattgatca
    43081 tcggtgccat cagtcaacaa gacttcactg cctgtggcca ggttcagcga agcgatacgg
    43141 aacgaaccgt cgcgtcttgc aacgtacagg agcgtcgatc catcggggga aattcggggt
    43201 gaaatgttgt aactgccatt aaacgtcaag cggcgcgctt cgctgccgtc caggccggtt
    43261 tggtatatct gcgggccgcc gctgcggtcg ctggtgaaga ccagcgaccg gccgtccggc
    43321 gtgaagaagg gctcggtgtc gatgccgggc gagcgggtca cgcggcgcat gctggagccc
    43381 ccgctggagg ggacgatata gatctgcgac aggccgtcgc gcgtcagcgc cacggccagc
    43441 tgggagccgt ccggcgacca ggccggcgcg ctgttgttgc ccttgaagtt ggccacgggc
    43501 gtctgggcgc tggtggccag ggtctgcaca tagaccaccg gcttgccggt atggaagctc
    43561 acataggcca ggcgggcgcc gtcgggcgac caggtgggcg agatgatggg ctcgcgcgag
    43621 cgcagcgcca cttgcgggtt ctgcccgtcg gcgtcggcca cctgcagctc gtag
//
LOCUS       DASWPO010000121         7683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2436619, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000121 DASWPO010000000
VERSION     DASWPO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2436619"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..2031)
                     /locus_tag="VGC69_07040"
     CDS             complement(58..2031)
                     /locus_tag="VGC69_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEY0295073.1"
                     /translation="MVAVAAPVIALIDLRDIRKSYGGADGSPATEVLRGVSLSLAAGE
                     FVAIMGASGSGKSTLMHILGCLDRPSSGEYRFAGQDIAAFSADELAWLRREAFGFVFQ
                     GYHLIRTLDALRNVEVPAVYAGQPAAEREARATRLLARLGLAERLGHRPSQLSGGQQQ
                     RVSIARALMNGGHIILADEPTGALDSKSGAEVMALLRELAEAGHTVILITHDRDVAAQ
                     ARRVIEIKDGQVIADSGAVQVPAAPSLAPESFAARMAAGVNHAASAWTDAREAMTSAW
                     RALSVNKFRTLLTLLGIIIGVASVIVMLAVGNGTQAQVIAKMAIYGTNRMYVIPGSDN
                     PRAPGGTLSEADVDVIRRVPGVADAMPFLKGQVTLRAGSVDTASSLWAVASRAPAILS
                     WHPVRGLFFTAKDEQELATVVVLGKKVRERLYGGRDPIGQYLLVNNVPFQIIGEMSEK
                     GAVTGDSDDDDVVLMPFSTGSRRVVGTTKLSWISVLVADIAKSDETAARITDALAAAH
                     RIQDFKVYNAAAAVKAQEDTQQTLTLMLGLIAAISLVVGGIGIMNIMLMTVRERTREI
                     GIRMATGARRRDIQRQFVSEAVVVSLVGGCAGVAIGLGLGAALIAWNVPVIFSVRGIL
                     GAFACALATGLVFGFMPARQAARLDPVVALASE"
     gene            complement(2025..3206)
                     /locus_tag="VGC69_07045"
     CDS             complement(2025..3206)
                     /locus_tag="VGC69_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0295074.1"
                     /translation="MSKNPIPLRPRIVVPALVIVLALLLWSLLRTREMIDTVAIGRGD
                     IEVSVTALGVIQPRSYVDVGAQASGAIRRIAAQPGSPVRQGDLLVEIDPSVQQAKVDA
                     DRAALDSLRAQLAEQEADRDLARQQYDRQRRMAAEGATRDEDLQSAQAALRVAEARIA
                     NLKAQIAGASSTLQGDQAQLGYTRIYAPMAGTVVTLDAREGQTINATYATPKILRIAD
                     LSTMTVWTQVSEADVRRVKPGMPVYFTTLGDDRRWTGKVRQILPAPPNPSESESSDSQ
                     KASSVPTAGKVVLYTVLFDVDNADGALMPQMSAQVFFVEAAAHGVVLAPLTAMQAEPG
                     KPDRYAAQVQESGGRLVAREVRTGVRDRLQAEVLSGLQPGDRLVTGIRHARAADGRFQ
                     W"
     gene            complement(3224..3415)
                     /locus_tag="VGC69_07050"
     CDS             complement(3224..3415)
                     /locus_tag="VGC69_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295075.1"
                     /translation="MTRSSWNIWRIPLALAALMVFGLLAALLGTGAWHWAAWLALALP
                     LMVGLWHACRRRPAATHSS"
     gene            complement(3412..4599)
                     /locus_tag="VGC69_07055"
     CDS             complement(3412..4599)
                     /locus_tag="VGC69_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0295076.1"
                     /translation="MTTLHFRNLRLWTAIHKWSSLVCTLFLLVICLTGLPLVFSEEIH
                     DWQEPRTYADLPAGAPNAGLDALASAARGLYPSEVVTSMFVDDDEPQVYAWMAPSFEE
                     AEKHPKSRHFIRFDARTAQVLEQSQPLGKRPMDFMDWMLRLHMDLFADLPGELFLAVM
                     ALLFVIAIVSGAVLYGPFMKKLDFGTVRAGRSRRLRWLDLHNLLGIVTLAWTFVVGAT
                     GLMNELSTPLFGLWQRTDVRAMLAPWQGKQAPALSELVSPQAALETAQRAAPGTTFTS
                     VVFPGDDNGSPHHYLLWGHGSSALTSRLFTPVLVDARSGELTAVVEMPWYLRALEVSR
                     PLHFGDYGGLPLKILWAALDLITIVVLASGLYLWFARRQATTARRDKLAHGVPATTPS
                     AMP"
     gene            complement(4646..7081)
                     /locus_tag="VGC69_07060"
     CDS             complement(4646..7081)
                     /locus_tag="VGC69_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0295077.1"
                     /translation="MNITTASRIRKRHPRLGRRRDMGALAISAALALGAMPVAARAQE
                     APVHVSIPAQSLGKALLQLGEQTSLQIFFSQDLVNGLNAPAVSGNLPPEQALQSLLRG
                     TAITYRREGNNVTLAQGNPSSVAQMPAIAVTGDAVQTEAQRLNPPTMVGSKTPLTQRE
                     IPQSVSVVTQEQIQAQNLDDMYDALRKTPGMVVTPYRDERVDLFSRGFPVDSMQLDGL
                     PLALNAANGLLAPDLAMYDHVETLRGPAGLYNGFGGPGGTINLVRKRPLDDLAASAEL
                     RLGTNHEARGMADLSTPLNQDKTVRARIVGSYESTDLYQDSTYKRPGLLYGIVEADLT
                     PATKVDIGGSYQRLSMRSMALGYPAYSNYQLIQNPKAYLGGTNDYDRYESNSAFFDVE
                     HRFGNDWKFKLAGTRLSNTADMRGSFPCCGGVDPATGITRMSWGGGDSGHNTQDVLDM
                     YVDGPFELAGRQHHLTLGMNYQRVDDYLRNIFGVTTATANLFDPVELPPYNGPLTTTA
                     TTTVTTQYTTYGNARFKLADPLTLVLGGNVMWWRASATPDADQNPFGSPTTRDQVNGH
                     LTPYGGLIYDLNQTYSAYASYSTVFTPQSARDKNGELIKPMQGNQYEVGLKGTYLDGK
                     LNTGLALFQLTQENRAVADPTEDPFSGISIAQGKARSRGLEASLSGEVYPGLSLYTGY
                     AYTSVRSLGSDDGGTEGIGAQTFSQIAPKHSFKLWADYRLPGSWSRYSVGAGLNVSSS
                     YYSDDGVAHLTQPGYMTADLKLGYVINEHMDVSLYIANIFNRRYYESIGSTWNQNFPG
                     APRTAMLTLRWKM"
     gene            complement(7315..>7683)
                     /locus_tag="VGC69_07065"
     CDS             complement(7315..>7683)
                     /locus_tag="VGC69_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY0295078.1"
                     /translation="GAVDVSAGPWWDRTTRQLTADQGVVTDAKGTLGQVAPVNAANVA
                     AWQRGKIVFEDMPLAQVVQEMNRYLPMPASFAAPGLAQYRVGGVFGIDDPLAMMNALP
                     AIAPVRVLQAPDGRLQVVGR"
BASE COUNT         1199 a         2521 c         2731 g         1232 t
ORIGIN      
        1 agccgcccag gaccaggagc gcggccagcg acaggaggca aggcgtccgg cgcgccatca
       61 ttcgctcgcc agcgccacga cgggatcgag ccgcgcggcc tggcgcgccg gcatgaaacc
      121 gaagacgagt cccgtcgcca gcgcgcaggc gaaggccccg aggatgcccc gcaccgagaa
      181 gatcaccggc acgttccagg cgatcagcgc cgcgcccagc cccaggccaa tggccacgcc
      241 cgcgcagccg cccaccagcg acaccaccac cgcctcggag acgaactggc gctggatgtc
      301 gcgccggcgc gcgccggtcg ccatgcggat gccgatttcg cgggtacgct cgcgcaccgt
      361 catgagcatg atgttcatga tgccgatgcc gcccacgacc agggagatcg ccgcgatcag
      421 ccccagcatc agggtcagcg tctgctgcgt gtcttcctgg gccttgaccg cggcggcagc
      481 gttgtagacc ttgaaatcct ggatgcgatg ggcggccgcg agcgcgtcgg tgatgcgcgc
      541 ggccgtctcg tcggacttgg cgatgtcggc gaccagcacc gagatccacg acagcttggt
      601 ggtgccgacc acgcgccggc tgcccgtgga gaagggcatc agcaccacgt cgtcgtcatc
      661 cgagtcgccg gtgacggcgc ctttctcgct catctcgccg atgatctgaa acggcacgtt
      721 gttgaccagc aggtactggc cgatcggatc gcggccgccg tacaggcgtt cgcgcacctt
      781 cttgcccagc acgacgacgg tggcgagttc ctgctcgtcc ttcgcggtga agaagagccc
      841 gcgcaccgga tgccagctca ggatggccgg tgcgcggctg gccaccgccc acaggctgga
      901 ggccgtgtcc acgctgccgg cgcgcagcgt gacctggccc ttgaggaagg gcatggcgtc
      961 ggccacgccg ggaacccggc gtatcacgtc cacgtcggcc tcggacagcg tgccgcccgg
     1021 cgcgcgcggg ttgtcgctgc cgggaatgac gtacatgcga ttcgtgccgt agatggccat
     1081 cttggcgatg acctgcgcct gggtgccgtt gcccacggcc agcatgacga tgaccgaggc
     1141 cacgccgatg atgatgccga gcagggtcaa cagcgtgcgg aacttgttga cgctgagcgc
     1201 gcgccaggct gacgtcatcg cctcgcgcgc gtcggtccag gcgctggcgg cgtggttcac
     1261 gcccgccgcc atccgggccg cgaagctctc cggcgcgagc gagggggcgg cggggacttg
     1321 caccgccccg gaatcggcga tcacctggcc gtccttgatc tcgatgacgc gccgggcctg
     1381 cgcggccacg tcgcggtcgt gggtgatgag gatgaccgta tggccggcct cggccagttc
     1441 gcgcagcaag gccatgacct cggcgccgct cttgctgtcc aaggcgcccg tgggctcgtc
     1501 ggccaggatg atgtggccgc cgttcatcag agcgcgggca atggagacgc gttgctgctg
     1561 tccgccggag agctggctgg ggcgatggcc caggcgctcg gccaggccca ggcgcgccag
     1621 cagcctggtg gcccgggcct cgcgttcggc cgccggctgg ccggcgtaga cggccggcac
     1681 ttcgacgttg cgcagcgcgt cgagcgtgcg gatcaggtgg tagccctgga agacgaagcc
     1741 gaaggcctcg cgccgcagcc aggccagttc gtcggccgag aaggcggcga tgtcctggcc
     1801 ggcgaagcgg tactcgcccg aactcggccg gtccaggcag cccaggatat gcatgagggt
     1861 ggacttgccc gacccggagg cgcccatgat ggcgacgaac tcgcccgcgg ccagggacag
     1921 cgacaccccg cgcagcacct cggtggcggg gctgccgtcc gcgccgccgt aggacttgcg
     1981 gatgtcgcgc aggtcgatca aggcaataac cggcgcggcc accgctacca ctggaatctc
     2041 ccgtcggcgg cccgcgcgtg ccggatgccc gtcaccagcc tgtcgcccgg ctgcaagccg
     2101 gacagcacct cggcctgcag gcggtcgcgc acgccggtgc gcacctctcg ggcgacgagg
     2161 cgcccgcccg attcctggac ctgggcggcg tagcggtccg gcttgccggg ttcggcctgc
     2221 atggccgtca gcggcgccag caccacgccg tgcgcggcgg cctcgacaaa gaacacctgg
     2281 gcgctcatct gcggcatcag cgcgccgtcg gcgttgtcca cgtcgaacag caccgtgtag
     2341 agcacgactt tgcccgccgt gggcacggat gacgccttct gcgaatccga tgattccgac
     2401 tcggagggat tgggcggcgc gggcaggatc tggcggacct tgcccgtcca gcgccggtcg
     2461 tcgcccaggg tggtgaagta caccggcatg cccggcttga cccggcgcac gtcggcctcc
     2521 gatacctggg tccacaccgt catggtggac aggtcggcga tgcgcaggat cttgggggtg
     2581 gcgtaggtgg cgttgatcgt ctggccctcg cgggcgtcga gcgtgaccac ggtgcccgcc
     2641 atgggcgcat agatgcgggt gtagccgagc tgggcctggt cgccctgcaa ggtggacgac
     2701 gcgccggcga tctgcgcctt caagttggcg atgcgggcct cggccacgcg cagcgcggcc
     2761 tgggccgatt gcaggtcctc gtcgcgggtg gcgccctcgg cggccatgcg gcgctggcgg
     2821 tcgtattgct ggcgggccag gtcgcggtcg gcctcctgct cggcgagctg cgcgcgcagg
     2881 ctgtcgagcg ccgcgcggtc ggcatcgacc ttggcctgtt gcaccgaggg gtcgatctcc
     2941 accagcaggt cgccttgcct gaccgggctg ccgggctggg ccgcgatgcg gcggatcgcg
     3001 cccgaggcct gggcgcccac gtccacgtag ctgcgcggct ggatcacgcc cagcgcggtc
     3061 acgctcactt cgatgtcgcc gcggccgatg gccacggtat cgatcatctc gcgcgtgcgc
     3121 agcaaggacc agagcagcaa ggccaggacg atgacgagag cggggacgac aatgcgcggg
     3181 cgcagcggaa tcggattctt ggacatcttg tgttgcgatg gcctcaggat gaatgggttg
     3241 cggccggccg gcggcggcag gcgtgccaga gacccaccat gaggggaagg gccagcgcca
     3301 gccaggccgc ccagtgccag gcgcccgtgc caagcagggc tgccagcagg ccgaacacca
     3361 tgagcgcggc aagcgccagc ggaatccgcc agatgttcca ggaggatcgg gtcatggcat
     3421 ggcactcggg gtcgtggccg ggacaccgtg cgccagcttg tcccgccgcg ccgtcgtggc
     3481 ctggcggcgg gcgaaccaga ggtagaggcc gctggccagc acgacgatgg tgatgagatc
     3541 gagcgccgcc cagaggatct tgagcggcag gccgccgtag tcgccgaagt gcagagggcg
     3601 cgacacttcg agcgcccgca agtaccaggg catctcgacc acggccgtca gctcgccgct
     3661 gcgcgcgtcc accaggaccg gcgtgaacag ccgcgaggtc aaggcgctgc tgccgtgccc
     3721 ccacaacagg tagtggtggg gactgccgtt gtcgtcgcca ggaaagacga cactggtgaa
     3781 cgtggtgccc ggcgccgcgc gctgcgccgt ctccagcgcg gcctggggcg aaaccagttc
     3841 cgacagcgcc ggcgcctgct tgccctgcca gggagccagc atggcccgga catcggtgcg
     3901 ctgccacagc ccgaaaagcg gcgtggacaa ctcgttcatg aggccggtcg cgcccaccac
     3961 gaaggtccag gccaaggtca cgatgcccag caggttgtgc aggtcgagcc atcgcaggcg
     4021 ccggctgcgg ccggcgcgca ccgtgccgaa gtcgagcttc ttcatgaagg ggccgtacag
     4081 cacggcgccg gacacgatgg cgatcacgaa caacagcgcc atcacggcca ggaacagttc
     4141 tccgggcagg tcggcaaaaa gatccatgtg caggcgcagc atccaatcca tgaaatccat
     4201 gggccgcttg cccagcggct gggattgctc cagcacctgg gccgtgcggg catcgaagcg
     4261 gatgaagtgc cgggatttgg gatgcttttc agcctcctcg aaggacggcg ccatccaggc
     4321 atagacctgg ggctcgtcgt cgtccacgaa catcgatgtc actacttcgc tcggatacag
     4381 gccgcgggcg gccgaggcca gcgcgtccag gccggcgttg ggcgcgccgg ccggcaggtc
     4441 cgcgtaggtg cgcggctcct gccagtcgtg gatctcttcg gaaaagacca ggggcaggcc
     4501 cgtcaggcag atcaccagca ggaagagggt gcagaccagg ctggaccatt tgtggatggc
     4561 cgtccaaagt ctcaggtttc gaaaatgcag cgtggtcatg ctcgtctctt gactgtgtgt
     4621 cctggggggc ctgtctcgtc gaccgtcaca tcttccagcg caaggtcagc atcgctgtcc
     4681 tgggcgcccc cgggaagttc tggttccagg tggagccgat gctttcgtag tagcgccggt
     4741 tgaagatgtt ggcaatatag agcgagacat ccatgtgttc gttgatgacg tagccgagct
     4801 tgagatccgc cgtcatgtag ccgggctgcg tcaagtgcgc cacgccatca tcggagtagt
     4861 agctgctgga gacattcaac ccggctccca cgctatagcg ggaccatgat ccgggaagcc
     4921 ggtaatcggc ccacagcttg aaggagtgct tgggggcgat ctggctgaat gtctgggcgc
     4981 cgattccttc cgtgccgccg tcgtccgagc ccaggctgcg cacgctggtg taggcgtagc
     5041 ccgtgtacag gctgagtccg gggtagacct cgcccgagag ggaggcttcc aggcctcggc
     5101 tgcgggcctt gccctgggcg atggaaatgc cggagaaggg atcctccgtc gggtcggcca
     5161 cggcccggtt ctcctgggta agctggaaca acgccaggcc cgtgttcagt ttgccgtcga
     5221 gatacgttcc cttgaggccg acttcgtact gattgccctg catcggcttg atcagctcgc
     5281 cgttcttgtc gcgcgcgctt tggggcgtaa agaccgtgct gtagctggca taggccgagt
     5341 aggtctggtt gaggtcgtag atcagcccgc catagggcgt gagatggcca ttgacctggt
     5401 cacgggtggt cgggctgccg aaggggttct ggtccgcatc gggcgtcgcg ctcgcccgcc
     5461 accacatgac gttgccgccc agcaccaggg tcagcgggtc ggccagcttg aaccgggcgt
     5521 tgccgtaggt ggtgtactgg gtcgtgaccg tggtcgtcgc cgtcgtggta agggggccgt
     5581 tgtaaggcgg gagctcgacc ggatcgaaga ggttggccgt ggccgtggtg acgccgaaaa
     5641 tattccgcag gtagtcgtcg acccgctggt aattcatgcc cagtgtcaga tgatgctgcc
     5701 gccccgccag ttcgaacggg ccatcgacat acatgtccag cacatcttgc gtgttgtggc
     5761 cgctgtcccc gccgccccat gacatgcgcg tgatgccggt ggcgggatcc acgccgccgc
     5821 agcagggaaa ggagccccgc atatcggccg tgttggacaa gcgggtgccg gccagcttga
     5881 acttccagtc gttgccgaaa cgatgctcga cgtcgaaaaa cgccgagttc gactcgtagc
     5941 ggtcgtagtc gttcgtgccg cccaggtagg ccttgggatt ctggatcagc tggtagttgc
     6001 tgtacgcggg atagcccagc gccatggaac gcatggagag ccgctggtag ctgccgccga
     6061 tgtccacttt ggtggcgggg gtgaggtccg cttccacgat gccgtacagc aggccagggc
     6121 gcttgtaggt cgagtcctgg tagagatcgg tgctttcata cgagccgacg atccgggccc
     6181 gcaccgtctt gtcctggttg agcggggtcg acaggtcggc catgccgcgg gcttcatggt
     6241 tcgtgcccag gcgcaattcg gcggatgcgg cgaggtcgtc caagggacgc ttgcgcacca
     6301 ggttgatggt gccgccgggg ccgccgaagc cgttgtacag gccggccggc ccgcgcagcg
     6361 tctcgacgtg gtcgtacatc gccaggtccg gcgccagcag gccgttggcc gcgttgagcg
     6421 cgagcggcag gccgtccagc tgcatcgagt cgacgggaaa gccgcgcgag aacaggtcca
     6481 cgcgctcgtc ccggtagggc gtcacgacca tgccgggcgt cttgcgcagg gcatcgtaca
     6541 tgtcgtcgag gttctgggcc tggatctgct cctgcgtcac gacgctgacg gactggggaa
     6601 tctccctttg ggtcagcggc gttttcgaac cgaccatcgt gggagggttg aggcgctgcg
     6661 cctcggtctg caccgcatcc cccgtgaccg cgatggccgg catttgcgcc accgacgatg
     6721 ggttgccttg cgccagggtc acattgttgc cctcgcgccg gtaggtgatg gcggtgccgc
     6781 gcagcagcga ctgcaaggcc tgttccggcg gcaggtttcc cgacaccgcc ggcgcgttca
     6841 atccattgac caggtcctgc gagaagaaga tctgcagcga ggtctgctcg cccagctgca
     6901 gcagggcctt gcccagggac tgggccggga tgctgacgtg gacgggcgcc tcctgcgcgc
     6961 gggcggcgac cggcatggcg cccagcgcca aggcggccga gatggccagg gcgcccatgt
     7021 cccggcgccg gccgaggcgc gggtgtcgtt ttctgatgcg gcttgcggtg gtgatgttca
     7081 cgatgcttcg tcttcccatg aatgtatgca gcgccgagcg gcgcccaacc cccttggcca
     7141 agggtctcgg cggggcgtat ggaaacgccc ccctcatgga aagaaacgaa tgaacccgcc
     7201 aagcaccgta atctaaatga gaactatttt gtaacggggc cgttccagcc tgccgcgcgg
     7261 acctccccga gcggggcagg ccgcggcacg tccggcatgt cggcggggcc ggcatcagcg
     7321 ccccacgacc tgcaagcggc catcgggcgc ttgcagcacc cgcaccggcg caatggccgg
     7381 cagcgcgttc atcatcgcca gcggatcatc gatgccgaac accccaccca cccggtactg
     7441 cgccaggcct ggcgcggcga atgaagccgg catcggcagg tagcggttca tctcttggac
     7501 gacctgcgcc agcggcatgt cctcgaacac gatcttgccg cgctgccagg cggcgacgtt
     7561 ggccgcattg acgggggcga cctggcccag ggttcctttg gcgtcggtga cgacgccctg
     7621 gtcggccgtg agctgccggg tcgtgcggtc ccaccagggg cccgcggaca cgtccacggc
     7681 ccc
//
LOCUS       DASWPO010000122         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3150683, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000122 DASWPO010000000
VERSION     DASWPO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3150683"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VGC69_07070"
     CDS             complement(<1..729)
                     /locus_tag="VGC69_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0295079.1"
                     /translation="MSNKKISKGGDVDAPTWPERLLGEGLVVLFLVGWWAMARGLPEF
                     VLPGPLAVGRRLLDLFINPEFLGHTAASTVRVVASVVIAGVLGGALAFLAAGVPVCEL
                     IVQERIKPVLNSFPAIGWAILAAIWFDVGDFSVIFVEVAILVPFCLVNISEGLRAIDG
                     DLMEMGRSFTRKAPRVWWRITLPLLVPYILSAIRIAYGIGWKIALVSELIGAPSGLGY
                     LMLRAQTVSDSVTFLATCFAIVLIF"
     gene            complement(719..1513)
                     /locus_tag="VGC69_07075"
     CDS             complement(719..1513)
                     /locus_tag="VGC69_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0295080.1"
                     /translation="MSTALSARHAAAGRARFSKRAAAHGFTLAFLAIWEAASWVLPPL
                     LLPGPGEVAVGLYRFLANPHQLWHLAVSIGHVLGAIVLSFAIGAALALLAYYVPVLRF
                     AIHRRLAPFLNAFPGVGWALLAVMWFGINSSSVVFAISAVLTPFALVNLAAGLANVDG
                     ELVEMAQSFSRNRFRQFTRVLVPLLYPFVFATLRIMFGVAWKVTLTAELFGGNAGLGY
                     LINMARQDFDTQTIFVAIILIVIFVYGVDRLVFSPLQNRLSRQYVQ"
     gene            complement(1523..2515)
                     /locus_tag="VGC69_07080"
     CDS             complement(1523..2515)
                     /locus_tag="VGC69_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEY0295081.1"
                     /translation="MHRRSVLKMAALPALMGVARPSFSQSRQRITYAYLLDPAYDCVT
                     WAMRNGKIPSDLITVETRGLAIPQLIQATSAKQYDVIMAAAISLPMAQERGLDVRVLA
                     TALRSAVAGEGSGVWVPKDSPIKTAQDLKGKTLGSYGLRSTGYTQIRIALNRKYGLNM
                     ALEGGDVQQVEIQAPNLPAALSTGKIDAATLIHSQAYRAMHSGEYRLIAETARDNNET
                     FKMQFISAVNIAYPERLAKQPQAYVEFCRMFRESKRYALANRAEVFGAVSKESGLEPA
                     FFDWWFEKNSDVPGYFSEEHAVAIMKFYELARDMGVLKSFPDIRTLVWDQAPRA"
     gene            complement(2515..>2833)
                     /locus_tag="VGC69_07085"
     CDS             complement(2515..>2833)
                     /locus_tag="VGC69_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295082.1"
                     /translation="PKIIFMDEPFSALDPNTRARMRVEIEQIWQRTGKTVVFVTHDID
                     EALQLADRIVLLSKKPTTVLETLIVDTPRPRRLDSAALAAQRDHLVSLFRAMETQSPS
                     GEA"
BASE COUNT          556 a          901 c          924 g          452 t
ORIGIN      
        1 gaaaataagc acgatggcga agcatgtcgc caggaacgtg acgctgtccg agacggtctg
       61 cgcccgcagc atcaggtatc ccagcccgct gggcgccccg atcagctccg acaccagggc
      121 gatcttccag ccgatgccgt acgcgatgcg gatcgccgac aggatatagg gcaccagcag
      181 cggcagcgta atgcgccacc aaacgcgcgg cgccttgcgg gtgaagctgc ggcccatttc
      241 catcaaatcg ccgtcgatgg cgcgcaggcc ctcgctgata ttgaccaggc agaaaggcac
      301 caggatggcc acttcgacga agatcacgct gaagtcgccg acgtcgaacc agatggccgc
      361 caggatggcc cagccgatgg cgggaaagga gttcaatacc ggcttgatgc gctcctggac
      421 gatcagctcg cacaccggca cgcccgcggc caggaaggcc agcgcgccgc ccaggacgcc
      481 ggcgatgaca accgaagcga ccacgcgcac ggtggaagcg gcggtgtggc ccaggaactc
      541 cggattgatg aacaggtcca gcaggcgcct gcccaccgcc aaggggccgg gcaggacgaa
      601 ttccggcaga ccgcgggcca tggcccacca accgaccagg aacagcacga ccagcccttc
      661 gcccagcagg cgttcgggcc aggtcggggc gtccacatcg ccgcccttgg aaattttctt
      721 attggacata ctgccgcgac aaccggttct gcaagggtga aaacaccagg cgatcgacgc
      781 cgtagacgaa gatgacgatc aggatgatgg cgacgaagat cgtctgggtg tcgaagtcct
      841 ggcgcgccat gttgatcaga tagcccaggc cggcgttgcc gccgaacagt tccgccgtca
      901 gcgtgacttt ccaggcgacg ccgaacatga tgcgcagcgt ggcgaacacg aagggataga
      961 gcaaaggcac gagcacgcgc gtgaattgcc tgaagcgatt gcgggaaaag ctctgcgcca
     1021 tctcgaccag ctcgccgtcg acattggcca gccccgccgc gaggttgacc agggcgaagg
     1081 gcgtcagcac ggcgctgatc gcgaacacca ccgagctgga attgatgccg aaccacatca
     1141 cggccagcag ggcccagccc acgcccggga acgcgttcag gaaaggcgcc agcctgcgat
     1201 ggatggcaaa gcgcaagacg ggcacgtaat aggccagcag cgcaagcgcg gcgccgatcg
     1261 cgaacgacag gacgatcgcg cccagcacgt ggccgatgga cacggccaga tgccacagct
     1321 gatgcggatt ggccaggaag cggtatagcc cgaccgcgac ttctccgggc cccggcaata
     1381 gcagcggcgg cagcacccat gacgccgctt cccatatcgc caggaaagcc agggtgaacc
     1441 catgcgcggc ggcgcgcttg gaaaagcgcg cgcgtcccgc ggcagcgtgg cgcgcgctca
     1501 atgccgtgga catcgctatg ccctacgccc ggggggcctg gtcccagacc aaggtgcgaa
     1561 tgtcggggaa ggacttcagc acgcccatgt cgcgcgccag ttcgtagaac ttcatgatgg
     1621 cgacggcgtg ctcttcgctg aaatagccgg gcacgtccga attcttttcg aaccaccagt
     1681 cgaagaaggc gggctcgagg ccgctttcct tcgacaccgc gccgaagact tccgcgcggt
     1741 tggccagcgc gtagcgcttc gattcgcgga acatgcggca gaactccaca taggcctgcg
     1801 gctgttttgc caggcgctcg ggataggcga tgttcaccgc gctgatgaac tgcatcttga
     1861 aggtttcgtt gttgtcgcgc gcggtttccg cgatcaggcg gtattcgccc gaatgcatgg
     1921 cgcgataggc ctggctgtgg atcagcgtcg ccgcgtcgat cttgccggtg gaaagcgcgg
     1981 ccggcaggtt cggcgcctgg atttccacct gctgcacatc gccgccttcc agggccatgt
     2041 tcaagccgta tttcctgttc agcgcgatgc gtatctgcgt atagccggtg gaccgcaggc
     2101 cataagagcc cagcgtcttg cctttcaggt cctgcgctgt cttgatggga ctgtctttcg
     2161 gcacccagac gccggagcct tcgcccgcca cggccgaccg cagcgccgtc gccaggacgc
     2221 gcacgtccag tccgcgctcc tgcgccatcg gcaggctgat cgccgccgcc atgatgacgt
     2281 cgtactgctt ggccgacgtc gcctggataa gctgcggaat ggccaggccg cgggtctcga
     2341 ccgtgatcag gtcggacggg atcttgccgt tgcgcatcgc ccaggtcacg cagtcataag
     2401 cgggatcgag cagataggcg taggtaatgc gctggcgcga ctgcgaaaaa ctggggcggg
     2461 ccacgcccat cagcgccgga agggccgcca ttttcaggac actacgacga tgcatcacgc
     2521 ttctccgcta ggggattggg tttccatggc ccggaacagg ctgaccagat ggtcgcgctg
     2581 cgccgccagc gctgcgctgt ccagcctgcg cggccgggga gtatccacga tcagtgtttc
     2641 gagcacggtt gtcggtttct tggaaagcag gacgatccgg tcggccagtt gcagggcttc
     2701 gtcgatatca tgcgtgacga acacgacggt cttgccggtg cgttgccaga tctgctcgat
     2761 ctcgacgcgc atgcgggcgc gcgtattcgg atccagcgcg gaaaacggct cgtccatgaa
     2821 aatgatcttg ggg
//
LOCUS       DASWPO010000123        15936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_26000, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000123 DASWPO010000000
VERSION     DASWPO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26000"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VGC69_07090"
     CDS             complement(<1..319)
                     /locus_tag="VGC69_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295083.1"
                     /translation="MPLSMKFTIFWIMAFGQFMALFDIQIVSASLNDVQAGLAAGPDE
                     VSWVQTAYLMAELVMIPLSGYLNRAVSTRWLFSMSAGLFTLSSLMCGMAWNIESMILF
                     RAVQ"
     gene            484..1629
                     /locus_tag="VGC69_07095"
     CDS             484..1629
                     /locus_tag="VGC69_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0295084.1"
                     /translation="MDNAARTAPAAGSASGDAAPAPLPRAKRGRPSRRTLLASGIALA
                     AIVAGALWIFARPASESTDDAYIGADTTTVAPKVRGLVEAVLVHDNQHVHAGEPLVRI
                     DPEEFDARVQSAQAALADADAQVAAAQAALASLDAEERLAEANVAAARTSIRSANATS
                     EQAKADYRRYTALIGSGAVAERDTDTYKTAAINAEQNAARAQAMLAVSERESGVTQAR
                     RATLEAARQKAQAQRASAVAALDLARQDQRHALILAPIDGVIGNRQVQVGDYVQPGSR
                     LLTLVPLNALYVTANFKETQTRDMRVGQAATIDIDALGHTLKGRVESFAPGSGSTFSL
                     LPFEPGTGNFTKIVQRIPVRIHFDADQPGADTLRPGLSVTAKVRLVD"
     gene            complement(1635..2669)
                     /locus_tag="VGC69_07100"
     CDS             complement(1635..2669)
                     /locus_tag="VGC69_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0295085.1"
                     /translation="MRYRVFGRTGLKVSSLSLGTGNFGTGWGYGSQRDEAKVVYDAYR
                     AAGGNFIDTADAYQFGQSEAMLSEFIAQDRDDIVLATKFALGSAPDSGLQGTGNSRKA
                     MVQSVEASLRRLKTDRIDLLWAHMPDGVTPAEEIVRGLDDLVRAGKVIYLGLSDFPAW
                     RVATAAMIAELRGWAPISALQLEYSLVERTVEQELLPMAAAFGLGTVGWSPLGGGLLT
                     GKYRKGETGRKEGLGAVIHDESSDTKTAAVDAVLAVAQALDVSPGQVAIAWVLSKGVL
                     PILGPRTPAQLADNLAAAQVKLDAGHIARLDAASAVPPGFPHGVVERRRNTLAGGKAD
                     LLDYPAVPVR"
     gene            complement(2831..3760)
                     /locus_tag="VGC69_07105"
     CDS             complement(2831..3760)
                     /locus_tag="VGC69_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295086.1"
                     /translation="MLDRMTGMQVFAKAAAMGSLSAAARQMAMSPAMAAKHLNALEDR
                     LGARLFHRSTRKLSLTEAGQQYLDACGRLLPELEEVEAAIASQRAQATGLLRLNTPLS
                     FGVRYIAPLLPAFCREHPGVVVDLGLNDRVVDLVEEGWDLTLRVGALKDSRLVARKLA
                     DSAMVVCAAPQYWARQGFPQTWAALSSHNCLSFSLSDLSRPDVWRYGRHGERRVAVRG
                     NLRANNGDALIAAAVAGLGVLYEPEFIVADALRGGLLASVQLDDEAADLGGIHLVRSA
                     DRMPPAKVRAMSDFLLEAFSPQPPWAGRGDRAP"
     gene            3872..5128
                     /locus_tag="VGC69_07110"
     CDS             3872..5128
                     /locus_tag="VGC69_07110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0295087.1"
                     /translation="MPSRTASTNPLPRNPSRREWLAATALGGLAMMAGCASPAASPWS
                     RTAAIDAVLDDATRSGHLVGATVIAAQDGKIVYRRAAGLADREAGRPVREDEIYRLAS
                     MTKAIVCVTALALADQGLLHLDDPVTRWLPDFRPRLPDGRAPVITLRHLLTHTAGLSY
                     GFLEAPDGPYHRLGVSDGMDASGLTLAQNLERIAAAPLLFEPGAGWHYSVAIDVLGAV
                     IERATGLPLPAAVQRLVTGPLGLSSLAFSVAPGTVLATPYGDATPQPARMTEPFSLKF
                     GGSAIVYSPARAFDANAFPSGGTGMVGTAADYLRFAEALRTGGSGLIRPETVAAMTRS
                     VTGKLAIGAAGPGFGWGLGVAVLTDPEAAKLPMPPGCWNWSGVYGTNFWVDPVNRLSV
                     VALTNTAVAGMTGDFPLALRRAVYAA"
     gene            5154..6173
                     /locus_tag="VGC69_07115"
     CDS             5154..6173
                     /locus_tag="VGC69_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0295088.1"
                     /translation="MQAYRLQAGAGIAGLARQELSSREPGPTEIRVRLDAAALNNRDL
                     SFANGLFYNPPTQAIVPLVDGCGEVVAAGAGVTRFKAGDRVITQYYPDWIEGELDPRK
                     TARSFGAQIDGTLAQEITADANAFAAAPRHLGAPAAATLTCAGLTAWNALFVAGAARP
                     GATVLLLGTGGVSLWALQLAKAAGLRVLVTSSQDEKLDRARRLGADGVINYRTQPYWE
                     DEILRLTEGRGVDLVVEVGGEGTLARSLRATAAGGCVVVIGRVSGGRGVMIEPGMLIG
                     GAKRLTGITTGSRAMLEQLVRFVDTNRIEPVVDTVFPFEEAPAAYAYLASGRHFGKVA
                     IDLQA"
     gene            complement(6191..7798)
                     /locus_tag="VGC69_07120"
     CDS             complement(6191..7798)
                     /locus_tag="VGC69_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295089.1"
                     /translation="MRIKSALQLSAVALAVSAAAFTTSATARTLHWAYQSDAVSLDPM
                     ILNETFTLGFQGNIYETLFGYDKDLKLVPVLAESWTNPEPTKWVIKLRQGVKFHDGSP
                     FTADDVIFSWKRSLTPGSNMKGFGSKATDIKKLDDYTVEITTAAPDPILPRDWTWLYM
                     MSKSWSEKNNTAQASDIKNPQGNYANLHENGTGPFMVADRQQDVKTTLKRFPDYWDKS
                     IKTNVDEVVFQPITQESTRVAALISGEMDIVQPVPVQDWERLKSTKGVKVLNAPEIRA
                     IFIGMDQWRDELLYSNVKGKNPFKDPKVREAVVLAVDTAAINQKIMRGAAKPLGSLVS
                     TALNGYDASYGSPIKADPAQSKKLLAEAGYPNGFEVTMDCPNDRYVNDEKICQAVAGM
                     LARVGIKIDLRAQTKSKFFAQVQLLPNTGSQTSMYLLGWSANTVDADNTLFNLIHCTD
                     FKAGAGMVNYGNYCNKDVDALEAQINSETDQAKRNELIKQAFAIVRKEWGYLPLHQQP
                     LSWGVKDTLKVAQRADDVLDLRNVVMP"
     gene            8107..8583
                     /locus_tag="VGC69_07125"
     CDS             8107..8583
                     /locus_tag="VGC69_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295090.1"
                     /translation="MKTTIVSLAFAAGLLASAAGFAQTAPAGAPAGATGLCKDGTYYT
                     GATKQGACRGHKGVGTWYGAATPAAAAPAAMAPAAAAAAPAAAATPGKKPAAFTPAAT
                     AAPGGGPGLVWVNTSSKVYHCQGDKWYGKTKAGSYMSEADAKTKGFHGPRGKTCGA"
     gene            8766..10361
                     /locus_tag="VGC69_07130"
     CDS             8766..10361
                     /locus_tag="VGC69_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295091.1"
                     /translation="MPLKPSHLIAAIGLAISMQAAHAAGTLIYCSEASPEGFDIAQFT
                     TGTTNDAAGHTVYNRLVDFKKGTLDLAPSLAQSWDISPDGLAYTFHLRKGVKFQTYSY
                     FKPTRDFDADDVVFTYTRLINKDAPFNKAYPAQFPYANDTGLVANVVSIDKVDDYTVK
                     FTLKTPDADLVAKMALPSMSILSAEYADKLLKEGKADMINQQPVGTGPFVLVRYEKDA
                     QLRYRANKEYWNPGVVKLDNLIFAITPDASVRAQKLKANECQIAAYPKPQDLADLAKA
                     PNLKMMEAPGFNIGYLAYNNEKPQLKDVRVRQALDMAINRKAILDAVYQGQGQEAANP
                     YPPSLWGYNAALRNPPMDIEKAKKLLADAGYPNGFELSLWALPVQRPYNPNGKLMAEM
                     IQSDWAKIGVKANIVTYEWGEYLKRMKNGEFDAGLMGWTGDYASPDNFLGVLLSCAAV
                     GGSNYSRWCDKDFDTLVVGARAISDQSKRAEMYSQAQAIFKQQVPWSTIAHSTTSQPL
                     AKNVEGYAISPLGRHIFEDVSIK"
     gene            10490..11518
                     /locus_tag="VGC69_07135"
     CDS             10490..11518
                     /locus_tag="VGC69_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0295092.1"
                     /translation="MGSFILRRLALLIPTFIGITLAAFSLIHLIPGDPVEVMMGERTL
                     DPDMHRQFMHQLGLDMPVYQQYFHYLANVLHGDLGISIVTRKSVWTEFLALFPATLEL
                     SICALLLALVLGILAGTLAAVKRGTVLDHGVMGLSLTGFSMPIFWWGLILIIVFSVTL
                     GWTPVSGRIDLAFDIPPRTGFMLIDAWLAQRADPDVNAGAFLDAVRHLILPSIVLGTI
                     PLAVIARMTRSSMLEVLREDYVRTARAKGMAPLRVVFVHVLRNALIPVITVIGLQMGT
                     LLGGAVLTETIFSWPGVGKWLIDAIGRRDYPVVQNGILIVATLVIITNLIVDVLYGVV
                     NPRIRHKR"
     gene            11518..12450
                     /locus_tag="VGC69_07140"
     CDS             11518..12450
                     /locus_tag="VGC69_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0295093.1"
                     /translation="MEDSRMTTTIAPAGTQPGQEQAAPSPLIDFINNFKRNRGAVIAL
                     AIMVVVVLAALLAPWVAPHDPLSQDRDHMLTPPVWAGSGQWTYLLGTDEAGRDILSRL
                     IYGARMSLLIGLTSVLFALVPGVALGLLATFFPKMAGPLIMRLMDIMMALPSLLLAVA
                     IMAVLGPGVLNAMIAIAIVSLPAYVRLTRASAMVELERDYVTASRMAGAGLLRLMFVT
                     ILPNCMAPLLIHATMSFSNAIIDIAALGFLGLGVQPPTPEWGTMLASARDYITSAWWV
                     VTMPGLTILVSVLSINLMGDGLRDALDPRLKRIA"
     gene            12466..13458
                     /locus_tag="VGC69_07145"
     CDS             12466..13458
                     /locus_tag="VGC69_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295094.1"
                     /translation="MSLLEIQDLSVEFGAPGSAFRAVKHVSFTVEPGEVLGIVGESGS
                     GKSVTMMALMDLLDENARVSAQAVRFKGQDLLSLSGRQKRALYGRDIAMIFQDPMTSL
                     NPSYTVGYQIGEVLRTHLGLRGAAARKRMLALLDLVEMPAAASRLGAYPHQLSGGMSQ
                     RVMIAMALAGDPSLLIADEPTTALDVTIQAQILDLLCDLQRERGMALIMITHDLAVVS
                     QVAQRVQVMYGGTLVEMQSAQSLFHDPSHPYTEALLRAIPERSQGASRLYALPGMVPG
                     KYDQPAGCILAPRCPYAQPRCHAEEPPMAALARADGGQALSKCFYPLNPQAGQP"
     gene            13455..14432
                     /locus_tag="VGC69_07150"
     CDS             13455..14432
                     /locus_tag="VGC69_07150"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY0295095.1"
                     /translation="MNEATVIQADRLSRHYTVSQGWLKPKGTVRALSDASFTLSAGRT
                     LAVVGESGCGKSTLARQLTLIEPPTGGKLVLGGIDVAQASKEQLKTLRTRVQMVFQNP
                     YASLNPRHTVGTQIGEPLAINENLAKAERRDRVRAIMARVGLRPEHDARYPHMFSGGQ
                     RQRIAVARAMILKPEIVVADEPTSALDVSIQAQVLNLFMDLQQELGTAYVFISHNLSV
                     VEHIADDVMVMYLGRVVERGPKAQVFAQPLHPYTHTLLAATPTLDGPRRAKRIRIESE
                     PPSPLNPPSGCAFHGRCPHADAQCSQQLPEPRLIDSRWVACHHVERITD"
     gene            complement(14422..15330)
                     /locus_tag="VGC69_07155"
     CDS             complement(14422..15330)
                     /locus_tag="VGC69_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295096.1"
                     /translation="MNKLQALQVLLEVAEAGGFAKAAQRLGVATSSVTRLIDALEAEL
                     GAALLTRTPRKVSLTDAGAAYTEQVAKVLDDLAEADGNVADSGGALVGTLRVSVPSVY
                     SRVRLAAHLADFMAAHPRVTLHIVADDHYADLAHDRFDVAIRIGLPAQDPRLIARTLV
                     DNPRYVVASPDYLARAGRPSRPAELATHACLRFAYGGNYRARQVWSFQRGGEQERVDV
                     KGRLTSNNADMLLSGVQGGQGLALLPVWLVEAAIADGRLAHLFPDWEATPHDDQAVVY
                     AAYLPNRRHSGKVHALIQFLASRLNP"
     gene            15486..>15936
                     /locus_tag="VGC69_07160"
     CDS             15486..>15936
                     /locus_tag="VGC69_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295097.1"
                     /translation="MPTPEPAAGSHVPRMTGGLVALFSFCCGAIVANIYYAQPITELI
                     APDLHMSVNTASLIVSLTQIGYALGLFFLVPLGDLLENRKLMLTTVLVAIVSLIAAAF
                     TRQPGWFLAFSLLVGISSVAVQMLIPLAVHFAPEATRGRIVGNIMSGL"
BASE COUNT         2613 a         5733 c         5006 g         2584 t
ORIGIN      
        1 cctgcaccgc ccggaagagg atcatcgact cgatgttcca ggccatgccg cacatcaggc
       61 tcgacagcgt gaacaggccg gccgacatgg agaacagcca gcgggtcgac acggcgcggt
      121 tgaggtagcc cgacagcggg atcatcacca gctcggccat gaggtaggcc gtctggaccc
      181 agctgacctc gtccggcccg gcggccagcc ctgcctggac gtcattgagc gacgccgaca
      241 cgatctggat gtcgaacaag gccatgaact ggccgaacgc catgatccag aagatcgtga
      301 acttcatgga caagggcatg gccgccgggt tgtcggacca atcacgtccc agggcgctca
      361 gttttccgaa catcttgccg ctttccctcg ttgcatcttt tgtatattat gatcgacata
      421 taaatgatga tcgtaataca aagatcgtcc ttccacaaga cacggatccc gacaggagaa
      481 aacatggaca acgcggccag gaccgcaccg gcagcaggca gcgcttccgg cgacgccgct
      541 cccgcccccc tgcctcgcgc caagcgcggc aggccctcgc gccggacgct gctggcatcc
      601 ggcatcgccc tcgcggccat cgtcgccggc gccctctgga tctttgcccg gcccgcctcc
      661 gagtcgaccg acgacgccta tatcggcgcc gacacgacca cggtcgcgcc caaggtccgg
      721 ggcctggtcg aagccgtgct cgtgcacgac aaccagcacg tgcatgccgg cgaacccctg
      781 gtgcgcatcg acccggaaga attcgacgcc cgggtgcaaa gcgcccaggc cgccctggcc
      841 gatgccgacg cccaggtcgc cgcggcgcag gccgcgctgg ccagcctgga cgcggaagaa
      901 cggctggccg aagcgaacgt ggcggcggcc cggacctcca tccgctcggc caatgccacg
      961 tcggaacagg ccaaggccga ctaccggcgc tacaccgcgc tgatcggcag cggcgcggtg
     1021 gccgagcgcg acacggatac ctacaagacg gccgcgatca atgccgagca gaatgcggcc
     1081 cgggcccagg ccatgctggc ggtctcggag cgcgagtccg gcgtgaccca ggccaggcgg
     1141 gcgaccctcg aagcggcgcg ccagaaagcg caggcgcagc gcgccagcgc cgtggccgcg
     1201 ctcgacctgg cccggcaaga ccagcggcac gcgctgatcc tcgcgccgat cgacggcgtg
     1261 atcggcaacc ggcaggtgca ggtcggggat tatgtgcagc cgggttcgcg cctgctgacg
     1321 ctggtgccgt tgaacgccct ctacgtcacg gccaatttca aggaaacgca gacccgcgac
     1381 atgcgggtcg gacaagccgc caccatcgat atcgacgcgc tgggccatac gctcaagggc
     1441 cgcgtggaaa gcttcgcccc cgggtccggc tcgacgttct cgctgctgcc cttcgagccc
     1501 ggcaccggca atttcacgaa gatcgtgcag cgcatccccg tgcgcatcca cttcgacgcg
     1561 gaccagcccg gcgcggatac cctgcgcccc ggcctgtccg tcaccgccaa ggtgcgcctg
     1621 gtggattgaa cccgtcagcg cacgggcacg gccggataat ccagcaggtc ggccttgccg
     1681 cccgccagcg tgttgcgccg gcgctcgacg acgccgtgcg ggaacccggg cggcaccgcg
     1741 ctggccgcgt cgagccgggc aatgtgcccg gcatcgagct tgacctgcgc cgccgccagg
     1801 ttgtcggcaa gctgcgcggg cgtgcgcggg ccgaggatgg gcagcacgcc cttggacagc
     1861 acccaggcga tggccacctg gccgggcgac acgtccagcg cctgcgcgac ggccagcacc
     1921 gcatcgacgg cggccgtctt ggtatcgctg ctctcgtcgt ggatcaccgc gcccagccct
     1981 tccttgcggc ccgtctcgcc cttgcggtac ttgccggtca gcaggccgcc gcccagcggc
     2041 gaccagccca cggtgcccag gccgaacgcc gccgccatcg gcagcagctc ctgctccacg
     2101 gtgcgctcga ccaggctgta ttcgagttgc agcgccgaga tgggcgccca gccgcgcagt
     2161 tcggcgatca tcgccgccgt cgccacgcgc caagcgggaa agtccgagag cccgaggtag
     2221 atcaccttgc cggcgcggac caggtcgtcg aggccgcgga cgatctcctc cgcgggcgtg
     2281 acgccgtcgg gcatgtgcgc ccacagcaga tcgatgcggt cggtcttcag tcgcctgaga
     2341 ctcgcctcga ccgactgcac catggccttg cggctattgc ccgtgccctg cagcccgctg
     2401 tccggcgccg aacccagcgc gaacttggtg gccagcacga tgtcgtcccg gtcctgggcg
     2461 atgaactcgc tgagcatggc ctcggactgc ccgaactgat aggcatcggc ggtgtcgatg
     2521 aagttgccgc cggccgcgcg gtacgcgtcg tacaccacct tggcctcgtc gcgctgggag
     2581 ccgtagcccc agcccgtgcc gaaattgccc gtgcccaggg aaagggatga aaccttcagg
     2641 ccggtacggc cgaaaacgcg gtaacgcatg gaagacctct atcgaagaaa agcgcgcaag
     2701 ccgcggcttg cctggcttgc gctaagttaa cttgcaaatt gcaagttaaa aatttaatag
     2761 aggtagcttg cattatgcaa gtaaaaaatc caggcgcgtt cctttcaatg agcggggttg
     2821 aggcccgata tcatggcgcg cggtctcccc ggccagccca tggcggctgc gggctgaacg
     2881 cctccagcag gaagtcgctc atcgcccgga ccttggccgg cggcatgcgg tcggccgaac
     2941 ggaccagatg aatgccgccc aggtcggccg cttcgtcgtc gagctgcacg gaggccagca
     3001 ggccgccgcg cagcgcatcg gccacgatga actccggctc gtacaacacg ccgagcccgg
     3061 ccacggccgc ggcgatgagc gcatcgccgt tattggcgcg caggttgccc ctgacggcca
     3121 cgcgccgctc gccgtgcctg ccgtagcgcc agacgtcggg acgcgacagg tcggacaagg
     3181 aaaaactcag gcaattgtgg ctggacaagg ccgcccaggt ttgggggaag ccctgccggg
     3241 cccagtactg cggcgcggcg caaacaacca tggcgctgtc ggccagcttg cgggcgacga
     3301 gccggctgtc cttgagcgct cccacgcgca gggtcaagtc ccagccctct tccaccaggt
     3361 cgaccacacg gtcgttcagc cccaggtcga cgaccacgcc cgggtgctcg cggcagaatg
     3421 ccggcagcag cggcgcgatg tagcgcaccc cgaacgacaa gggcgtattc aagcgcagca
     3481 gccccgtcgc ctgcgcgcgc tgcgatgcaa tggcggcctc gacttcctcc agctcgggca
     3541 acaggcggcc gcaggcatcc aggtattgct ggccggcctc ggtcaggctc agcttgcgcg
     3601 tgctgcggtg aaaaagccgg gcgcccagcc ggtcttccag cgcgttcagg tgcttggccg
     3661 ccatggcggg cgacatagcc atctggcggg cggccgccga caagctgccc atggcggcag
     3721 ccttggcaaa aacctgcatt cccgtcatgc gatcaagcat atttatcacc atcagttgaa
     3781 actgaaataa ataatatgcc aattatcgaa tcaagggttg aggattaaag ttcaagcctt
     3841 gtcgaacccc acaccatcag gccttttccc catgccatcc cgcaccgcct cgaccaaccc
     3901 gctcccccgc aacccgtctc gccgggaatg gctcgccgcc acggccctgg gcggcctggc
     3961 catgatggcc ggctgcgcct cgccggccgc ctcaccctgg agccggaccg cggcgataga
     4021 cgccgtcctc gatgacgcca cgcgcagcgg ccacctcgtc ggcgccaccg tcatcgccgc
     4081 gcaggacggc aagatcgtct accggcgcgc cgccgggttg gccgaccgcg aagcgggccg
     4141 gcccgtgcgc gaagacgaga tctaccgcct ggcctcgatg accaaggcca tcgtctgcgt
     4201 cacggccctg gcgctggccg accaaggcct gctccacctg gacgatcccg tcacccgctg
     4261 gctgccggat ttccgccccc ggctgcccga tgggcgcgcg cccgtcatca cgctgcggca
     4321 tctgctgacc catacggccg gcttgagcta tggctttctc gaagcgcccg atggccccta
     4381 ccaccggctg ggcgtctccg acggcatgga tgcctcgggc ctgacgctcg cgcagaacct
     4441 ggagcggatc gccgccgcgc ccctgctctt cgaaccgggc gcgggctggc attactccgt
     4501 ggcgatcgac gtgctgggcg ccgtgatcga acgcgcgacc ggcctgccgc tgccggccgc
     4561 cgtgcagcgc ctcgtcaccg ggccgctggg cctgtcgagc ctggccttta gcgtcgcccc
     4621 cggcaccgtc ctggccaccc cctacggcga tgccaccccg cagccggccc gcatgaccga
     4681 gccgttctcg ctgaaattcg gcggcagcgc catcgtctac tcgcccgccc gcgccttcga
     4741 cgcgaacgcg tttccctcgg gcggcaccgg catggtgggc acggcagccg attacctgcg
     4801 ctttgccgaa gccttgcgca cgggcggatc cggcctcatc cgccccgaaa ccgtggcggc
     4861 catgacgcgc agcgtcacgg gcaagctggc catcggcgcg gccggccccg gcttcggctg
     4921 gggcctgggc gtggcggtcc tgaccgaccc cgaggccgcc aagctgccca tgccgccggg
     4981 ctgctggaac tggtccggcg tgtacggcac gaacttctgg gtcgaccccg tgaaccgcct
     5041 gtcggtcgtt gccttgacga acaccgcggt cgccggcatg acgggcgact tccccctggc
     5101 gctgcgccgc gccgtctacg ccgcatgatt cacgattgaa agggagtacc gccatgcaag
     5161 cctatcgact gcaagccggc gccggcatcg ccggcctcgc gcgccaggaa ctgtccagcc
     5221 gcgaaccggg ccccacggaa atccgcgtgc ggctcgatgc cgccgcgctg aacaaccgcg
     5281 acctcagctt cgcgaacggc ctgttctaca acccgcccac gcaggccatc gtcccgctgg
     5341 tcgacggctg cggcgaagtc gtggccgccg gcgccggcgt cacgcgcttc aaggcgggcg
     5401 accgggtcat cacccagtac tacccggact ggatcgaggg cgaactggac ccgcgcaaga
     5461 cggcccgatc cttcggcgcc cagatcgacg gcacgctggc ccaggaaatc acggccgatg
     5521 cgaacgcctt cgccgccgcg ccgcgccatc tcggcgcccc ggcggccgcc acgctgacct
     5581 gcgcgggcct gaccgcctgg aatgccctgt tcgtcgccgg cgcggcccgc cccggcgcga
     5641 ccgtgctgct cctgggcacc ggcggcgtat cgctctgggc gctgcaactg gccaaggccg
     5701 ccggcctgcg cgtcctggtc acgtcctcgc aggacgaaaa actggaccgg gcgcggcgcc
     5761 tgggcgccga cggcgtcatc aactaccgca cccagcccta ctgggaggac gaaatcctgc
     5821 ggctcacaga aggccggggc gtcgacctcg tggtcgaagt gggcggcgaa ggcacgctgg
     5881 cgcgttcgct gcgggccacc gcggcgggcg gctgcgtcgt ggtcatcggc cgcgtcagcg
     5941 gcggccgcgg cgtcatgatc gagcccggca tgctgatagg cggcgccaag cgcctgacgg
     6001 gcatcacgac aggcagccgc gccatgctgg aacaactggt gcgcttcgtc gacaccaacc
     6061 ggatcgagcc ggtcgtcgac acggtattcc cgttcgagga agcgcctgcc gcgtatgcgt
     6121 acctggcctc gggccggcac ttcggcaaag tggcgatcga cctgcaggcc tgatcgccac
     6181 ccgccgacgc tcagggcatc acgacgttgc gcaggtcgag cacgtcgtcg gcgcgctggg
     6241 ccaccttgag cgtgtccttc acgccccagg acagcggctg ctggtgcagg ggcaggtagc
     6301 cccactcctt gcgcacgatg gcgaaggcct gcttgatcag ctcgttgcgc ttggcctggt
     6361 cggtctcgct gttgatctgc gcctcgagcg cgtcgacgtc cttgttgcaa tagttgccgt
     6421 aattgaccat gcccgcgccg gccttgaagt cggtgcagtg gatcaggttg aagagcgtgt
     6481 tgtcggcatc gaccgtgttc gccgaccagc ccagcagata catgctggtc tggctgcccg
     6541 tattgggaag gagctggacc tgggcgaaga acttggactt ggtctgcgcg cgcaggtcga
     6601 tcttgatgcc gacgcgggcc agcatgccgg ccacggcctg gcagatcttc tcgtcgttga
     6661 catagcggtc gttggggcag tccatggtca cttcgaagcc gttggggtag ccggcctcgg
     6721 ccagcagctt cttggactgg gccggatcgg ccttgatcgg ggagccgtaa gaggcgtcat
     6781 aaccgttcag cgcggtcgac accagcgagc ccaggggctt ggcggcgccg cgcatgatct
     6841 tctggttgat ggcggcggtg tccaccgcca gcaccaccgc ttcgcgcacc ttcggatcct
     6901 tgaaggggtt cttgcccttg acgttggaat agagcagctc gtcgcgccac tggtccatgc
     6961 cgatgaagat ggcgcggatt tccggcgcgt tgagcacctt cacgcccttg gtgctcttga
     7021 ggcgttccca gtcctgcacc ggcacgggct gcacgatgtc catctcgccc gagatcagcg
     7081 cggccacgcg ggtggattcc tgcgtgatgg gctggaagac gacttcgtcg acgttggtct
     7141 tgatgctctt gtcccagtag tcggggaagc gcttgagcgt ggtcttcacg tcctgctggc
     7201 ggtcggccac catgaacggg cccgtgccgt tctcgtgcag gttggcgtag ttgccctggg
     7261 ggttcttgat gtcgctggcc tgggcggtgt tgttcttttc gctccaggat ttgctcatca
     7321 tgtagagcca ggtccagtcg cgcggcagga tggggtcggg cgcggccgtg gtgatctcga
     7381 cggtgtagtc gtcgagcttc ttgatgtcgg tggccttgga gccgaagccc ttcatgttgg
     7441 agccgggggt gaggctgcgc ttccacgaga agatcacgtc gtcggcggtg aagggcgagc
     7501 cgtcgtggaa cttcacgccc tggcgcagct tgatcaccca cttggtgggc tcggggttgg
     7561 tccagctttc ggccaggacg ggcacgagtt tgagatcttt gtcgtagccg aacagggttt
     7621 cgtagatgtt gccctggaaa cccaatgtaa acgtttcgtt gaggatcatc gggtccagcg
     7681 agacggcatc gctctggtaa gcccagtgca gcgtgcgcgc ggtggcgctg gtggtgaaag
     7741 cggcggcaga aacggccagt gccacggcgg acagctgcaa tgctgatttg atacgcataa
     7801 ccccttgttc tccatattgg aaatgttccc ttgcagatcc tatagaggct gtcatttgag
     7861 cgtcaatcac ctggcattgg tattgtcccg atacccaagg gttattcctc gccatgaaag
     7921 ttcccgggat gccggacatt tacaaacagc aaaacgcgca gggaaaacca ctaggcgcgc
     7981 tattgactcg gcgctaccat gccgcgcagt aataccggat accgtagtga tgtgtttgtc
     8041 tcccgcttgt cctgtcccgc ccacaaggct caacaagagt tggttcattt cccaaggaaa
     8101 cacccaatga agaccaccat cgtttccctc gctttcgccg cggggctgct cgcctcggct
     8161 gccggcttcg cgcagaccgc ccccgccggc gccccggccg gggccaccgg cctgtgcaag
     8221 gacggcactt actacaccgg ggccaccaaa cagggcgcct gccgcggcca caagggcgtg
     8281 ggcacctggt acggcgccgc cacgccggcg gcagcggcgc cggccgcgat ggcgccggcc
     8341 gccgccgcgg ctgcgcccgc cgccgctgcc acccccggca agaagcccgc cgccttcacc
     8401 cccgccgcca ccgcggcgcc tggcggcggc cccggcctgg tctgggtcaa caccagcagc
     8461 aaggtctatc actgccaggg cgacaagtgg tatggcaaga ccaaggccgg ttcgtacatg
     8521 tccgaagccg acgccaagac caagggcttt cacggcccgc gcggcaagac ctgcggcgcc
     8581 tgatacaggc cgccccggtc cttcgccagc cgtcctgcgg cggctggcgg cacaagaatc
     8641 ccccgctcgg gcttgcccgc cggggggttt tcatttcagg gcttgcccta gggccgattc
     8701 ccctggaaca accatgcgcc cctgttaaac tttcccatct gttgctattt ttatccggag
     8761 ctgtcatgcc cctcaagcct tcccatctga tcgcggccat cggcctggcc atttccatgc
     8821 aggctgccca tgccgccggc acgctgatct actgttccga ggccagcccc gaaggcttcg
     8881 acatcgccca gttcaccacc ggcacgacca acgacgccgc cggccacacc gtctacaacc
     8941 gcctggtcga cttcaagaaa ggcacgctcg acctcgcgcc ctcgctggcc cagtcctggg
     9001 acatctcgcc cgacggcctg gcctacacct tccacctgcg caagggcgtg aagttccaga
     9061 cctactccta tttcaagccc acccgcgact tcgacgccga cgacgtggtc ttcacctaca
     9121 cgcgcctgat caacaaggac gcccccttca acaaggccta cccggcgcag ttcccctacg
     9181 ccaacgacac cggcctggtg gccaacgtgg tgtcgatcga caaggtcgac gactacaccg
     9241 tcaagttcac cctgaagacc cccgacgccg acctggtggc caagatggcc ctgccgtcga
     9301 tgtccatcct gtcggccgag tacgccgaca agctgctcaa ggaaggcaag gccgacatga
     9361 tcaaccagca gccggtcggc accggcccct tcgtgctggt ccgctacgaa aaagacgccc
     9421 agctgcgcta ccgcgccaac aaggaatact ggaaccccgg cgtcgtcaag ctcgacaacc
     9481 tgatcttcgc catcacgccc gacgcctcgg tgcgggccca gaagctcaag gccaacgagt
     9541 gccagatcgc cgcctatccc aagccgcagg acctggccga cctggccaag gcgcccaacc
     9601 tgaagatgat ggaagccccg ggcttcaaca tcggctatct cgcctacaac aacgagaagc
     9661 cccagctcaa ggacgtccgc gtgcgccagg cgctcgacat ggccatcaac cgcaaggcca
     9721 tcctcgacgc cgtctaccag ggccagggcc aggaagccgc caacccctat ccgccgtcgc
     9781 tgtggggcta caacgccgcg ctgcgcaacc cgccgatgga catcgagaag gccaagaagc
     9841 tgctggccga cgccggctac cccaacggct tcgaactgag cctgtgggcc ctgcccgtgc
     9901 agcgtccgta caaccccaac ggcaagctga tggccgagat gatccagtcc gactgggcca
     9961 agatcggcgt caaggccaac atcgtcacct acgagtgggg cgaatacctc aagcgcatga
    10021 aaaacggcga attcgacgcc ggcctgatgg gctggacggg cgactacgcc agcccggaca
    10081 acttcctggg cgtgctgctg tcctgcgccg ccgtgggcgg ctcgaactac tcgcgctggt
    10141 gcgacaagga cttcgacacc ctggtcgtgg gcgcccgcgc catcagcgac cagtccaagc
    10201 gcgccgagat gtacagccag gcccaggcca tcttcaagca gcaagtgccg tggtcgacca
    10261 tcgcccactc caccaccagc cagcccctgg ccaagaacgt ggaaggctat gcgatcagcc
    10321 cgctgggccg ccacatcttc gaagacgtga gcatcaagta atccagtaga atcgcggact
    10381 caccgcattt aacggcccgg ccagcgttgc ctgtcatcat ggcaacctgg ccgggcagtt
    10441 ttttgcctct acccatcacg ctgcatcgat acatcggcag gaggccatca tggggtcttt
    10501 catcctgcgc aggctggcgc tgctcatacc gaccttcatc ggtatcacgc tggctgcgtt
    10561 ttccctgatc catctgattc ccggcgatcc ggtcgaagtc atgatgggcg agcgcacgct
    10621 cgaccccgac atgcacaggc agttcatgca ccagctcggc ctggacatgc cggtctacca
    10681 gcagtatttc cattacctgg ccaatgtgct gcatggcgac ctgggcattt ccatcgtcac
    10741 gcgcaaaagc gtctggacgg aattcctggc gcttttcccc gccacgctgg aactgtcgat
    10801 ctgcgcgctg ctgctggccc tggtgctggg catcctggcc ggcacgctgg ccgccgtcaa
    10861 gcgcggcacc gtgctggacc acggcgtcat gggcctgtcg ctcaccggct tctccatgcc
    10921 gatcttctgg tggggactga tcctcatcat cgtgttctcg gtcaccctgg gctggacgcc
    10981 ggtgtccggg cgcatcgacc tggccttcga catcccgccg cgcacgggct tcatgctgat
    11041 cgacgcctgg ctggcccagc gggccgaccc cgacgtcaac gccggcgcct tcctggacgc
    11101 cgtgcgccac ctgatcctgc cgtcgatcgt gctgggcacc atccccctgg cggtgatcgc
    11161 ccgcatgacc cgttcgtcca tgctcgaggt gctgcgcgaa gactacgtgc gcaccgcccg
    11221 cgccaagggc atggcgccgc tgcgcgtggt gttcgtgcat gtgctgcgca acgccctgat
    11281 tcccgtcatc accgtgatcg gcctgcagat gggcacgctg ctgggcggcg ccgtgctcac
    11341 cgagaccatc ttctcctggc cgggcgtggg caagtggctg atcgacgcca tcggccgccg
    11401 cgactacccc gtggtgcaga acggcatcct gatcgtggcc acgctggtca tcattaccaa
    11461 cctcatcgtc gacgtgctgt acggcgtggt gaacccgcgc atccggcaca agcgctgatg
    11521 gaggactccc gcatgacgac gactatcgca ccggccggca cgcagcccgg ccaagagcag
    11581 gcggcgccct cgcccctgat cgatttcatc aataatttca agcgcaaccg cggcgcggtg
    11641 atcgccctgg ccatcatggt ggtggtggtg ctggccgccc tgctggcgcc ctgggtggcg
    11701 ccgcacgacc cgctctcgca agaccgcgac cacatgctca cgccgcccgt ctgggccggc
    11761 agcggccagt ggacctacct gctgggcacc gacgaagccg gccgcgacat cctctcgcgg
    11821 ctgatctacg gcgcgcgcat gtcgctcctg atcggcctca cctcggtgct gttcgccctg
    11881 gtccccggcg tggccctggg attgctggcg accttcttcc ccaagatggc gggtccgctc
    11941 atcatgcgcc tgatggacat catgatggcg ctgccctcgc tgctgctggc cgtggccatc
    12001 atggccgtgc tcgggcccgg cgtgctcaac gccatgatcg cgatcgccat cgtttcgctg
    12061 ccggcctatg tgcgcctgac gcgcgcctcg gccatggtcg agctcgaacg cgactacgtc
    12121 acggcctcgc gcatggccgg cgccggcctc ttgcggctga tgttcgtgac catcctgccc
    12181 aactgcatgg cgcccctgct catccacgcc accatgagct tctccaacgc catcatcgac
    12241 atcgccgcgc tgggcttcct gggcctgggc gtgcagccgc ccacgcccga gtggggcacc
    12301 atgctggcct cggcgcgcga ctacatcacc agcgcctggt gggtcgtcac catgcccggc
    12361 ctgaccatcc tggtttccgt tctttcgatc aacctcatgg gcgacggcct gcgcgatgcg
    12421 ctcgaccccc ggttgaagcg catcgcctga ggcaggagcg ccgctatgtc tctactggaa
    12481 atacaagatc tcagcgtcga attcggcgcc cccggctcgg ccttccgcgc cgtcaagcac
    12541 gtgagcttta ccgtggaacc cggcgaagtg ctgggcatcg tgggcgaatc gggctcgggc
    12601 aagtcggtca ccatgatggc cctgatggac ctgctcgacg agaacgcccg cgtcagcgcc
    12661 caggccgtgc gcttcaaggg gcaggacctg ctgtccctga gcgggcggca gaagcgcgcg
    12721 ctctacggcc gcgacatcgc catgatcttc caggatccga tgacctcgct caaccccagc
    12781 tacaccgtgg gctaccagat cggcgaagtg ctgcgcaccc acctgggcct gcgtggcgcg
    12841 gccgccagga agcgcatgct ggcgctgctc gacctggtgg aaatgcccgc ggccgcttcg
    12901 cgcctgggcg cctacccgca ccagttgtcg ggcggcatga gccagcgcgt gatgatcgcc
    12961 atggcgctgg ccggcgaccc cagcctgctg atcgccgacg agcccaccac ggcgctggac
    13021 gtcaccatcc aggcgcagat cctcgacctg ctgtgcgacc tgcagcgcga gcgcggcatg
    13081 gcgctcatca tgatcacgca cgacctggcc gtggtgtcgc aggtggccca gcgcgtgcag
    13141 gtgatgtatg gcggcacgct ggtggagatg caatcggcgc agtcgctctt tcacgacccc
    13201 tcgcacccct ataccgaggc gctgctgcgc gccattcccg agcgcagcca gggcgcgagc
    13261 cgcctctatg ccctgcccgg catggtgccc ggcaagtacg accagccggc cggctgcatc
    13321 ctcgccccgc gctgccccta cgcgcagccg cgctgccatg ccgaggagcc gcccatggcc
    13381 gccctggccc gggccgacgg cgggcaggcc ctgtccaaat gcttctaccc cctgaacccg
    13441 caggcaggcc aaccatgaac gaagcgaccg tcatccaggc cgaccggctt tcgcgccact
    13501 acaccgtctc gcaaggctgg ctcaagccca agggcaccgt gcgcgcgttg agcgacgcca
    13561 gcttcacgct gtcggccggc cgcaccctgg ccgtggtggg cgaatccggc tgcggcaagt
    13621 ccaccctggc ccgccagctc accctgatcg agccgcccac cggcggcaag ctcgtgctgg
    13681 gcggcatcga cgtggcccag gccagcaagg aacagctcaa gacgctgcgc acccgcgtgc
    13741 agatggtgtt ccagaacccc tatgccagcc tgaacccgcg ccacaccgtg ggcacccaga
    13801 tcggcgagcc gctggccatc aacgaaaacc tcgccaaggc cgaacgccgc gaccgcgtgc
    13861 gcgccatcat ggcccgcgtg ggcctgcgcc ccgagcacga cgcccgctac ccgcacatgt
    13921 tctccggcgg ccagcgccag cgcatcgccg tcgcccgcgc catgatcctc aagcccgaga
    13981 tcgtggtggc cgacgagccc acctcggcgc tggacgtgtc catccaggcc caggtgctca
    14041 acctgttcat ggacctgcag caggagctcg gcacggccta tgtgttcatc tcgcacaacc
    14101 tgtcggtggt cgagcacatc gccgacgacg tgatggtgat gtacctgggc cgcgtggtcg
    14161 agcgcggccc caaggcgcaa gtgttcgccc agccgctgca tccctacacg cacacgctgc
    14221 tggccgccac gcccacgctg gacggcccgc gccgcgcaaa gcgcatccgc atcgagagcg
    14281 agccgcccag cccgctcaac ccgcccagcg gctgcgcctt ccacggccgc tgcccgcacg
    14341 ccgatgcgca gtgctcgcag cagttgcccg agccgcgcct gatcgactcg cgctgggtgg
    14401 cctgccacca cgtggaacgc atcacggatt gagccgggat gccaaaaact ggatcagcgc
    14461 gtgcaccttg ccggaatggc gccggttcgg caggtaggcg gcatacacca cggcctggtc
    14521 gtcgtggggc gtggcctccc aatccgggaa gagatgcgcc aggcgcccgt cggcaatcgc
    14581 agcctcgacc agccagaccg gcagcagggc caggccctgc cctccctgca ccccgctcag
    14641 cagcatgtcg gcattgttgg aggtcagccg tcccttcacg tcgacgcgct cctgctcgcc
    14701 gccccgctgg aaactccaga cctggcgggc ccggtaattg ccgccgtagg caaagcgcag
    14761 gcacgcatgc gtagccagct ccgccggcct ggatggccgc cccgcccggg ccaggtaatc
    14821 cgggctggcc acgacatagc gggggttgtc caccagggtc cgggcgatga gccgcggatc
    14881 ctgcgccggc aggccgatgc ggatcgccac gtcgaagcgg tcgtgcgcga ggtccgcata
    14941 atggtcgtcg gccacgatgt gcagcgtgac gcgcggatgc gccgccatga aatccgcgag
    15001 gtgcgccgcc agcctgaccc ggctgtagac cgagggcacg ctcactcgca gcgtgcccac
    15061 cagggccccg ccgctgtccg ccacgttgcc gtccgcctcg gccaggtcat ccaatacctt
    15121 ggcgacctgc tcggtatagg cggcgcccgc gtcggtcagg ctgactttgc gcggcgtgcg
    15181 cgtcagcagc gcggcgccca gctcggcctc cagggcatcg atcaagcggg tgacggacga
    15241 tgtcgccacg cccaggcgct gcgcggcctt ggcgaagccg ccggcttcgg cgacttccag
    15301 cagcacctgc aaggcctgca gcttgttcat ggctcgcctc ggcccggaaa ccgcgccctc
    15361 tccacaacgt tgcaatccgt gcaaccatac attgcataaa ttgattattc aactattttt
    15421 ccgcaacgat taagctgtac gccattgctg gattgcagca tcaaccaaga aggatccgtt
    15481 tcatcatgcc tacccccgaa cccgctgccg gcagccacgt gccccgcatg acgggcgggc
    15541 ttgtcgccct tttctcgttt tgctgcggcg ccatcgtcgc caacatctac tacgcgcaac
    15601 cgatcaccga gctcatcgcg cccgacctgc acatgtcggt caacacggcc agcctcatcg
    15661 tctcgctgac gcagatcggc tacgccctgg gcctgttctt cctggtgccg ctgggcgacc
    15721 tgctggaaaa ccgcaagctc atgctcacga ccgtgctcgt ggccatcgtc agcctgatcg
    15781 ccgccgcgtt cacgcgccag ccgggctggt tcctggcctt ctcgctgctg gtgggcatca
    15841 gctcggtcgc cgtgcagatg ctgatccccc tggccgtgca cttcgcgccc gaagccaccc
    15901 gcgggcgcat cgtcggcaac atcatgagcg gcctgc
//
LOCUS       DASWPO010000124         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2793919, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000124 DASWPO010000000
VERSION     DASWPO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793919"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VGC69_07165"
     CDS             complement(<1..1845)
                     /locus_tag="VGC69_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0295098.1"
                     /translation="MPVSRQALVLGLLAVAASQPVWAQSDVSKTLLEQGRYWQAQGRS
                     ELAEQSWAKLLTTDPNQPEALYGMGAIAVGKQQLTQAQTYLAKLRAAAPSSRYVPLLE
                     QDLRLAVEPGKSALVKARQMAQDAGTQQDKAAMSAAIAQYDLALGGKTPQGAVAREYY
                     TNLGYAQDGLDRAIQGLQRLNAEDPNNPAILMPLAQHMTSDDARRVDGLHLLEKLSTR
                     PDTGGAATEYWRGVLGWMGPPRPQDKPLFEAYLKLHPDDDEIRRQMLQPPSAGAGGSV
                     HQDPRLVRGAAAIKSGDLDTAEQAFSDVLREKPDSADALGGLGVVRMQQDKLAQAESL
                     LSRAVSRPGGGGWVRALNTARYWTLVNQANASQSAGDTDLAQRQLDQAMKLDAHASAA
                     RNALAKMYAEQGDLAKAENTYRAVLASDPGNRDAVSGLVGVMAQTGRADQAMAWINSM
                     TPDQQTSVGGLGRLRAAAASGQAKAAEQRGDMDAARQALEDARRNDPENPWIRLDLAS
                     YYLDNDQQSEARSLADSLASSPETPDTLYARALLASQMGEWSKAQETVARIPAADRTV
                     SMQTLARQIDFLAQADHASKLAKDGQQQEARELLTQLEPEAGQNPAMLG"
     gene            complement(1874..>3386)
                     /locus_tag="VGC69_07170"
     CDS             complement(1874..>3386)
                     /locus_tag="VGC69_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEY0295099.1"
                     /translation="PSPLQGPTLAVVTNPSDPNGKLLLVMGRDSKELKQAAEALAAGS
                     QALTGANALITQFADQAPRKPYDAPRWMRTDRPTSFGELVDTKKLNVSGYSPDIIRID
                     LRVPPDLFGWHEPKVPIDLHYRYTPQQYLVDSSLLFSVNDMFVKSVPLPSLERIEGGD
                     KISAEVLPDSSLPREALLQVPLEALKSRSQLQFRYMYDYIKQGECRDIIIDNVRGMID
                     PESTIDLSHYPHFIAMPDLAAFAQAGYPFTRMADLSETAVVLGANAGPQEYAAYLAAM
                     GRMGESTGYPVTGVTVLRGKSVDAAPAGKDLLVIASGTDQTWLNSWAEDMPAAYNRTS
                     RFSISDLPGRVLGWFHSDPRLDVEPNRLSMAYTGPGTSAVLAGFESRKEKGRSVVLMA
                     SNKPEGLNDVVLALLGGEGYERPIQGSLVAIRGKEVDPLVGEHTYYIGQLGGLQRLDW
                     WLSSLYPGLSLNYVAKILGIGLLVLLLLWALISWLRRSMRGPKESMQAYEGEE"
BASE COUNT          480 a         1162 c         1154 g          590 t
ORIGIN      
        1 gcccagcatc gccggattct gccccgcctc gggttcgagc tgggtcagca gttcgcgcgc
       61 ttcctgctgc tggccgtctt tcgccaattt cgatgcgtgg tcggcctggg ccaggaagtc
      121 tatctgccgc gccagcgtct gcatcgacac cgtgcgatcg gccgcgggga tgcgcgccac
      181 ggtttcctgc gccttgctcc actcgcccat ctgcgacgcc agcaaggcgc gggcatacag
      241 ggtgtccggc gtttccgggc tggacgccag gctgtcggcc aggctgcgcg cctcggactg
      301 ctggtcattg tccaggtagt agctggccag gtcgaggcgg atccaggggt tttcgggatc
      361 gttgcgccgg gcgtcttcca gcgcctggcg ggcggcgtcc atatcgccgc gctgctcggc
      421 cgccttggcc tggccggatg cggcggccgc gcgcaggcgc cccaggccgc cgacgctggt
      481 ctgctggtcg ggcgtcatgc tgttgatcca ggccatggcc tgatcggcgc ggccggtctg
      541 ggccatcacg cccaccaggc cgctgaccgc gtcgcggttg cccgggtccg aggcgagcac
      601 ggcgcgatac gtattctcgg ccttggccag atcaccctgt tccgcataca tcttggccag
      661 cgcattgcgc gcggccgagg catgcgcgtc cagcttcatc gcctgatcca gctgccgctg
      721 cgccaagtcg gtgtcgccgg cgctttgcga ggcattggcc tggttcacca gggtccagta
      781 gcgcgccgtg ttcaaggcgc ggacccagcc gcctccgccg ggacgcgaca cggcgcgcga
      841 cagcagcgac tcggcctgcg ccaacttgtc ctgctgcatg cgcaccacgc ccagcccgcc
      901 cagcgcatcc gcgctgtcgg gtttctcgcg cagcacgtcg ctaaaagcct gctcggccgt
      961 gtcgagatcg ccgctcttga tcgccgccgc gccgcgcacc aggcgcggat cctggtgcac
     1021 cgaaccgccg gccccggccg agggaggctg caacatctgg cggcggatct cgtcgtcatc
     1081 ggggtgcagt ttgagatacg cctcgaacag aggtttgtcc tgaggccgcg gcggacccat
     1141 ccagcccagc acgccccgcc aatattccgt ggccgcgccg ccggtatcgg gacgggtgga
     1201 gagtttctcc aggagatgca ggccgtccac gcgccgcgcg tcgtcgctcg tcatgtgctg
     1261 cgccagcggc atcaggatgg ccgggttgtt gggatcttcg gcgttcaggc gctgcaagcc
     1321 ctgaatggcc cgatccaggc cgtcctgggc ataaccgagg ttggtgtagt actcgcgcgc
     1381 cacggcgccc tgcggcgtct tgccgcccaa ggccaggtcg tactgcgcga tcgccgcgga
     1441 catggcggcc ttgtcctgct gggtaccggc gtcctgcgcc atctggcgcg ccttcaccag
     1501 cgcggacttg cccggctcga ccgccagccg caggtcctgc tccagcaagg gcacatagcg
     1561 gctggacggc gcggccgcgc gcagcttcgc caggtatgtc tgcgcttgcg tgagctgctg
     1621 cttgccgacg gcgatcgccc ccatgccgta gagcgcctcc ggctgattcg gatcggtcgt
     1681 cagcaacttg gcccaggact gctcggccag ctcggaccgg ccctgggcct gccaatagcg
     1741 accttgctcc agcagcgtct tggagacgtc gctctgcgcc cagacgggct ggctggccgc
     1801 gacggccagc aatcccagga ccaaggcttg acggcttacc ggcatggtga tcccttcatt
     1861 ctcaagccca gactcattct tcgccctcgt aggcctgcat ggactccttc gggccgcgca
     1921 tgctgcgccg cagccagctg atcaaggccc acaacaggag caataccaac aggccgatgc
     1981 ccagaatctt ggccacgtag ttcagcgaca ggccgggata cagcgacgac agccaccagt
     2041 cgaggcgctg cagcccgccg agctggccga tgtaataggt gtgttcgccc accagcgggt
     2101 cgacttcctt gccgcggatg gcgaccaggc tgccctggat gggccgctcg tagccctcgc
     2161 cgcccagcag cgccagcacg acgtcgttca ggccttcggg cttgttgctg gccatcagca
     2221 ccacgctgcg ccccttttcc ttgcgcgact cgaagccggc cagcaccgcg ctggtgcccg
     2281 gcccggtgta ggccatggac aagcggttgg gctcgacgtc caggcgcggg tcggaatgga
     2341 accatcccag cacgcgaccc ggtaggtcgg agatggagaa gcgcgaggtg cggttgtagg
     2401 cggcgggcat gtcctcggcc cagctgttga gccaggtctg gtcggtgccc gaggcgatca
     2461 ccagcaggtc cttgccggcc ggcgcggcgt ccaccgactt gccgcgcagc accgtcacgc
     2521 cggtcaccgg atagccggtg gactcgccca tgcggcccat cgcggccaga taggcggcgt
     2581 actcctgcgg accggcgttg gcgcccagca ccacggccgt ctcggacaga tcggccatgc
     2641 gcgtgaacgg atagccggcc tgcgcgaagg cggccagatc gggcatggcg atgaagtgcg
     2701 gataatggct caggtctatg gtcgactcgg ggtcgatcat gccgcgcaca ttgtcgatga
     2761 tgatgtcgcg gcactcgccc tgcttgatgt agtcgtacat gtagcggaac tgcaactggc
     2821 tgcgcgactt gagcgcttcc agcggcactt gcaacagggc ttcgcgaggc aggctcgagt
     2881 cgggcaggac ctcggcgctg atcttgtcgc cgccttcgat gcgctccagc gagggcagcg
     2941 gcaccgactt cacgaacatg tcgttcacgc tgaagagcag cgacgagtcc accaggtact
     3001 gctgcggcgt gtagcggtag tgcaggtcga tgggcacctt gggctcgtgc cagccgaaca
     3061 gatcgggcgg cacgcgcagg tcgatgcgga tgatgtcggg gctgtagccc gacacgttca
     3121 gcttcttggt gtcgaccagt tcgccgaagg acgtaggccg gtcggtgcgc atccacctgg
     3181 gcgcgtcgta aggcttgcgc ggcgcctggt cggcgaactg cgtgatgagc gcgttggcgc
     3241 cggtcaaggc ctggctgccc gcggccaggg cctcggccgc ctgcttcagt tccttgctgt
     3301 cgcggcccat caccagcaat agcttgccgt tggggtcgct ggggttggtg accaccgcca
     3361 gcgtcggccc ttgcagcggc gacggc
//
LOCUS       DASWPO010000125        17422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_651609, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000125 DASWPO010000000
VERSION     DASWPO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 17422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17422
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651609"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGC69_07175"
     CDS             complement(<1..257)
                     /locus_tag="VGC69_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEY0295100.1"
                     /translation="MGQLESSFKTSRSSLKIVQSVIFALFLREMQTRFGARRMGFVWV
                     VMDPLTILVVILVFHAVMNTGVFQGGQDEIMFMVSGIVPFH"
     gene            complement(460..1116)
                     /locus_tag="VGC69_07180"
     CDS             complement(460..1116)
                     /locus_tag="VGC69_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0295101.1"
                     /translation="MAGDVLKVMISDDSGATQTVFSPLAQGGTVFDQVRVAADGTISL
                     PYVGSRQVKGTTLQQAENIIRRGAKGWAASPQVHVEMVGNVSGSVLVAGAVKTPGRFS
                     SLQGPLTILDAINQAGGPSMEPFLIKVTLRTGKTVHVYNYQDLLSGRNREVPPNSEII
                     LDRDRRRFIAMGAVAQPGLHDFPSATPSLLEVLGTVGGLNERMANPSGVFIFRLTDAA
                     "
     gene            complement(1424..2542)
                     /locus_tag="VGC69_07185"
     CDS             complement(1424..2542)
                     /locus_tag="VGC69_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295102.1"
                     /translation="MSIFKKLLKNRLFTGMVIIPMLIIIIYFVFLARDRYVSTSSVVV
                     RSESSDSGSSTVQLPGIASLLGGGGGGASMMETLYVNEFVNSQDMLDILQKKLQWNQH
                     YAGNWLDSWYFLPTDAPQEDQLKYYQRMITTNYNEVNGLLTIKVQSFDPAFAKKTLDV
                     IISESDKFVNELSHKMAREQVAFAQEELERARKTYEQKRKIMLDFQSAHNVLDAQQSA
                     ISRNEVISTLQSNLTNANATLRAMRATLSENSPQVNQQKVMVIALQQQIAAEEQKLVA
                     KTNNNQLNVIASQYHALEVDAGIAEDNYKATVASLESARVEATKKLRSMSVIVSPNMP
                     DDNLYPRRAFDIFTVLVILLLLYGIAIFFIATIKDHQD"
     gene            complement(2864..4903)
                     /locus_tag="VGC69_07190"
     CDS             complement(2864..4903)
                     /locus_tag="VGC69_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0295103.1"
                     /translation="MLVSESWMASKAATRHHAYLVRGEVTGHAKAHVVDLILELNTGE
                     THAMPVLVTRKGLVLELVKVPGFVRSMRLAEQMLQGKVEQLSCRPLRAAEYRLRQIYR
                     VTRSIVKHPAKRRGRVGLTVKTLLTNLDKAYDAAVRFRTALRRSDYHEWHKEFYSISD
                     HDRRVMARRLARWKTPPRFHITILTQASGEAALQATRESLATQIYSHFDVVELPADRW
                     SCAWAARQATGQDRWCMVLRAGTQLAEQALYCLADSALRRPNASVIYTDHDSVSPKGD
                     LHVPIFKPDWSPELLRSTNYIGETFAWNSQHAACLHVAPVADSHIDTLHGMLLSLTAG
                     DTPVVHTPAPLLHLPPEPVHADDTSHVVAQYLRQLRIPATVVATSRDTCRVHYPVAQP
                     APMVSIIIPTRDELDHLQRCVSSVLEKTQYQVFEILVVDNQSRDPATLEYLQTLSKLP
                     NVRVLPFDRPFNYSKINNMAVREAKGELVCLLNNDTEVISPDWLDEMAGLLLRNDVGA
                     VGAKLLFDNGTVQHAGDTVGPGGCANHLHSGIGADEPGYNGRALVAQDLSSVTAACLL
                     TKKAVYQSLGGLNEANLTVAFNDVDYCLNVRQMGLRVVWTPHALLYHHESITRGKDET
                     RAQLRRAKKEVRYMRKRWAHVMQNDPFYNPNLNYLQPDFALSATPNVLKPWADSF"
     gene            complement(4903..6957)
                     /locus_tag="VGC69_07195"
     CDS             complement(4903..6957)
                     /locus_tag="VGC69_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEY0295104.1"
                     /translation="MSSTDLPTRRHDSATQAHASAHYAAELGRPRLYTCAPGIQRIST
                     LAALLNDFALQYRPKGLDGQGSVLAWGLRPSAQRAQRYAKKHGLGVYHIEDGFLRSVG
                     LGQDSPPLSIVLDDLGLYLDAAQPSRLEALIKRPLISRRTERAQALIRAWCDGQVSQY
                     NHSRDLYQSLPEQCVLVVDQTRGDASISGGQADERSFARMLKSALDEHPGSTILLKTS
                     PDVVAGRKQGHFDLAAARAMPRVKVLDADVHPASLLFRVQQVYTVTSQLGFEALLWGV
                     PVRVFGMPFYAGWGLTKDDMPMPLRRGPVPLEQLVHATLIDYSRYVDPETGKRCEVET
                     LLAWLALQRRMRQRFDTQVDAVGFTGWKKRHARAFFQGSGVSFQRRARVTNNHAVAVW
                     GNTQDASLGRKPIVRISDGFLRSVGLGADRTRPLSWVQDELGIYYDATRPSRLEELLL
                     HTEFSAELIARATLLRHAICAAGLTKYNLPGPRGLPRPNGQHVILVPGQVEDDASVQF
                     GASNIRSNMGLLRAVREDNPDAYIFYKPHPDVVSGLRRAGQDEERAAAWCDEIVGQVP
                     IQHLLDIVDEVHVLTSLTGFEALLRKVPVVTYGQPFYAGWGLTHDTDLSQSLRRRRTR
                     VLDLDELVAGALILYPTYVSRVTGQFTTPERVLRELIEWRAKPGVPRWRHWLARLFRE
                     RT"
     gene            complement(6944..7492)
                     /gene="rfbC"
                     /locus_tag="VGC69_07200"
     CDS             complement(6944..7492)
                     /gene="rfbC"
                     /locus_tag="VGC69_07200"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY0295105.1"
                     /translation="MKVVDTAIADVKILEPSVFGDARGFFYESFNQKRFDEAIGRKVD
                     FVQDNHSRSSRGVLRGLHYQMPPHPQGKLVRVVRGEVYDVAVDIRRSSPTFGTWVGVL
                     LSEENKRQFWIPEGFAHAFMVVSESADFLYKTTDYWSKELERSILWSDPQLNIDWPPG
                     IEPLLSEKDAQACPFAQADVFD"
     gene            complement(7489..8397)
                     /gene="rfbA"
                     /locus_tag="VGC69_07205"
     CDS             complement(7489..8397)
                     /gene="rfbA"
                     /locus_tag="VGC69_07205"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEY0295106.1"
                     /translation="MFDARSLQVKKRKGIILAGGSGTRLYPATLAMSKQLLPVYDKPM
                     IYYPLSVLMLAGLQDILIISTPQDTPRFQQLLGDGSEWGINLQYAVQDSPDGLAQAFL
                     IGERFLDGAPSALILGDNIFYGHDIANLLEQAGKREAGATVFAYHVHDPERYGVVEFD
                     KYGRALSIEEKPTAPRSNYAVTGLYFYDARVVDYAKSIKPSPRGELEITDLNQIYMEK
                     SLLDVVTMGRGYAWLDTGTHDSMMEAGQFIATIEARQGLKVACPEEIAYRLGYIDAIQ
                     LDKLADPMKKNGYGKYLKNILAEVRV"
     gene            8885..9628
                     /locus_tag="VGC69_07210"
     CDS             8885..9628
                     /locus_tag="VGC69_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295107.1"
                     /translation="MSRVAYFMDDELLRPTTWAGLPKPYAKKLRAYCLARAGDIRGLA
                     TDYWFSTRALQRSHGHLPSEVLPPRPLAQDRAAPRIWRASHTSEAPLLVFYHGSAAHQ
                     AEIAWLRPVMAEVLARCPNVHFEIIGDHEVNKRFRGLPRTRVVHPMGWPSYLNHCRTL
                     AGHIGLAPLLDTPFNAGRAHVKAFDISRCGAMGVYSAQGPYGQVIRDGENGLLLDNLP
                     ELWVEAITSLVNDTPRLDTLRRAAMALLG"
     gene            9591..9929
                     /locus_tag="VGC69_07215"
     CDS             9591..9929
                     /locus_tag="VGC69_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0295108.1"
                     /translation="MRCGARRWRCWDRQKPVPLATPGEILLHEWLEPMGISQYALAKA
                     IDVPPRRINEIVKGERAISADTAVRLGIFFGTDAQSWMNLQTHYDTETAREKIGHDKL
                     AELERHALAA"
     gene            complement(9942..10715)
                     /locus_tag="VGC69_07220"
     CDS             complement(9942..10715)
                     /locus_tag="VGC69_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0295109.1"
                     /translation="MRTAVPDCVLITGAAGGIGSALALEYARAGTATLILHARDAGQL
                     AEVAGQCAALGARVITAGIDARDHQALMQWLGEVGASAPPDLVLVNAGVNIHAEAGTQ
                     DESWEDMHRLFDVNVKAAMATVHAVVPAMRARGSGQIALVSSLAAWRGLPETPSYCAS
                     KAAIKVFGEALRDGLAGQGIKVNVIMPGYVESKMCFAMPGPKPFLWTAARAATYIRKG
                     LQANRARISFPFPLNLGCFLLAGIHPAVSGWFLRRLGYD"
     gene            complement(10783..11277)
                     /locus_tag="VGC69_07225"
     CDS             complement(10783..11277)
                     /locus_tag="VGC69_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY0295110.1"
                     /translation="MAKSKTSLPPRINIGISEKDRAAVAAELSKLLADSYTLYLSTHN
                     FHWNVTGPLFNTLHTMFMDQYTEEWGALDSIAERIRALGHYAPGTYREYAKLSSIEEP
                     KAVPEAMDMVRFLVAANEAVAKTARAAFTKADTANDQPTADLLTQRLDIHEKNAWMLR
                     SLLQ"
     gene            complement(11359..12303)
                     /locus_tag="VGC69_07230"
     CDS             complement(11359..12303)
                     /locus_tag="VGC69_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295111.1"
                     /translation="MTLTELKYIVAVARERHFGRAAEACFVSQPTLSVAIRKLEDELG
                     VTLFERGGTEVGVTPMGQRIVAQAQKVLEESASIKEIARQGHDPLAGPLRVGVIHTVG
                     PYLLPYLVPAQIERTPQMPLVLQENFTVRLLELLRQGEIDCAIMALPLPEAGLVMQPL
                     YDEPFIVAVPQDHELAKLKAIDAQDLKKQNMLLLGTGHCFRDQVLEVCPELSRFSAAS
                     DGIQRTFEGSSLETIRHMVAAGIGVTVLPMTAVPEIRPEKSLLRYVPFAGHVPDRRVV
                     LAWRRSFPRMAAIEALSQAVYACGLPHVTMLSDEPVSA"
     gene            complement(12350..14422)
                     /gene="recG"
                     /locus_tag="VGC69_07235"
     CDS             complement(12350..14422)
                     /gene="recG"
                     /locus_tag="VGC69_07235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY0295112.1"
                     /translation="MAPSSAVSRKPPAAEAGLSAIERKFHALGLRHPQDFILHLPLRY
                     EDETRITPIARLRPGFPAQVEGDILRSEVLYRPRRQLTAVIADDTGELQLRWLNFYGS
                     QHKQLTAGRRLRARGEVRGGLWGKEMVHPRMTHADTPLPQALTPVYPSTDGLPQPTLR
                     KAIDQALLQTDLSDTLPDSVRQRYGLEAFEPAIRLLHAPPPGVAEADLIERAHPAWRR
                     IKFDELLAQQLSLAAARAGRRSKQALSMPAPAQPGNLVQRLHDSLPFRLTQAQQRVSA
                     EIAQDLGLGYPMHRLLQGDVGSGKTVVAAMAAAQVIANGAQVALMAPTEILAEQHYRK
                     LSAWLEPLGVQVAWLSGSLPAKARRETAAAVSGGAVQLVVGTQALVQDHVSFRKLGLS
                     IVDEQHRFGVGQRLALSRKGEEGALAPHQLHMSATPIPRTLAMTFFADLDVSVIDELP
                     PGRTPVLTKLAADARREEVLMHVAQAARQGQQAYWVCPLVEESEALQLQTAVDTFDAM
                     RADLPDLRIGLVHGRLPQAEKAAVMQAFRQGEIDLLVATTVIEVGVDVPNASLMVIEH
                     AERFGLAQLHQLRGRVGRGTAESVCVLLYQHPLSQVARERLRAMFETNDGFEIARRDL
                     AQRGPGEFLGTRQSGLALLRFADIELDAAIAEQAREAAAWLRQHDPKAVQAHLDRWMR
                     GREDFLRT"
     gene            complement(14465..14851)
                     /locus_tag="VGC69_07240"
     CDS             complement(14465..14851)
                     /locus_tag="VGC69_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEY0295113.1"
                     /translation="MSKQIIHTDAAPAAVGPYSQAVAATGSKTVYLSGQIGLEPATGE
                     LVAESFEAQVRQAFANLTAVVDAAGGTLDGIVKLTLFLTDLGKFALANAIMAEIIPQP
                     FPARSTVGVASLPKGAQFEVEAVLVI"
     gene            15045..15557
                     /locus_tag="VGC69_07245"
     CDS             15045..15557
                     /locus_tag="VGC69_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295114.1"
                     /translation="MLAFDTFKYFHALTKAGVTQAEAEAHTHALVEAINANLQNLATK
                     DDLKPFATKDDLKVFATKDDLKLFATKDDLKPFATKDDLKLFATKDDLKPFATKDDLK
                     QLEDRMEGKMQQFATRVQLEELRGEVRASELRIQAQIAPMRWMLAATGTAVLAMFVNT
                     FASPLFKMLH"
     gene            complement(15567..16346)
                     /locus_tag="VGC69_07250"
     CDS             complement(15567..16346)
                     /locus_tag="VGC69_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0295115.1"
                     /translation="MKLITSRDNPLVKTLRKLATTAGRHGEPVLLDGVHLCQAWLARY
                     GAPEYAVFDSEKLQHPELHALAGQLPDSACIALEPRLMRDLASVESGQGVLFLVRPPE
                     PEPPVRLAENCVLFDRIQDPGNVGTLLRTCAAAGIKRVFLSTGTAAAWSPKVVRSGQG
                     AHFALAIHEHQDLLALLPRLDVPLVATALDGAVDLYAHPLPRHCAWAFGHEGQGVAPG
                     LLQAAALRVRIPHDAEAVESLNVAAAAAVCLFEQYRVHGRR"
     gene            complement(16369..16974)
                     /gene="rnhB"
                     /locus_tag="VGC69_07255"
     CDS             complement(16369..16974)
                     /gene="rnhB"
                     /locus_tag="VGC69_07255"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY0295116.1"
                     /translation="MQTNLFSTEANIDASTTAGVDEAGRGPLAGAVYAAAVILDPARP
                     IAGLADSKVLSAARREKLAGLIKAQSLAWCIAHASVDEIDQLNILRATLLAMQRAVEG
                     LSVAPRLALVDGNQPPRLRCAVQTIIKGDAKVPAISAASILAKTARDADLMRLHALYP
                     QYAFDKHKGYGTAQHLSLLREHGPCPEHRRSFAPVRVLLQP"
     gene            complement(17082..>17422)
                     /locus_tag="VGC69_07260"
     CDS             complement(17082..>17422)
                     /locus_tag="VGC69_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY0295117.1"
                     /translation="TLETALYKRPMVISYVLSPWMRRIMAWKSGQERPYLPWVGLPNI
                     LLRDSAVPELLQDDATPQALEAATWTALTDDALAASLETRFTAMHQELLRDTPTLAAQ
                     AIAAVADGRV"
BASE COUNT         3329 a         5265 c         5443 g         3385 t
ORIGIN      
        1 aagtgaaaag ggacgattcc acttaccata aacataatct cgtcttgccc cccctgaaaa
       61 actccggtat tcatcacagc gtgaaaaacc agaatgacga ctaagattgt caacgggtcc
      121 atcaccaccc aaacaaaccc catgcgccgc gccccaaatc gtgtttgcat ttctcgcaaa
      181 aacaaggcaa atatcactga ctgaacaatt ttcagtgatg atcgacttgt tttaaaagaa
      241 gattcgagct gtcccatgta tctccctctt agaaattgtc taccgtgcgg cccagcacta
      301 acacttgaac aataggggcg atgatcttct ccatctcata gacagcagcg ttggtcacat
      361 acactgtatc gtctggaaga atcaaaaaat tctttgccaa gaacatagat actggttcac
      421 gcatatccaa gtgaaaaact tcagccttgg atttttctcc taagcagcat cagtcaggcg
      481 aaatataaat acaccactgg ggttagccat acgctcgtta agccctccca cagtccccag
      541 cacttccaat aaactaggtg tagctgaggg aaagtcatgt aaccccggtt gggctaccgc
      601 acccatggcg ataaagcgcc ttcgatcgcg atcgagaatg atttcagagt tgggcggtac
      661 ctctctattc cgaccggaaa gtaaatcttg atagttatac acatgcacgg tcttcccagt
      721 gcgcagggta actttgataa gaaaaggctc catagaagga ccacccgctt gattaatagc
      781 atcgaggata gtcaatgggc cttgtaagga cgagaatcgg ccaggagtct tgacagcgcc
      841 agctaccagc accgaaccag agacgttgcc taccatctct acatgtacct ggggtgaggc
      901 ggcccaaccc ttagcaccac ggcgaatgat attctcggcc tgctgcaacg tagtaccctt
      961 gacttgcctg gaaccaacat acggtagcga gatagtgcca tccgccgcta cccgtacctg
     1021 gtcgaatact gtgccacctt gggccaaagg ggagaacacg gtttgggtgg cgccgctatc
     1081 atccgaaatc atgactttga gcacgtcgcc cgccatgaga tgaatctcgg gtgtactcgt
     1141 ggcagccaca tgatctcgca gcgctggttc ggctgggcgc atatacgaag caatgttgtt
     1201 agaggacaag tcaatcagat cgtacccgta gtcagaattg acaacgttgt catctttatt
     1261 ggcatcgcta gtaatactgc caattctcgg cccggcgcca gacagtaggc cgcaaccact
     1321 caaggagacc atactgatca atagtagagt ggatccgatt aattgacgga cagagtacat
     1381 tttcctaatg agaaaaaata tatttttaga gtgcataatc gcttcagtct tggtggtctt
     1441 tgatagttgc aatgaaaaat attgcaatgc cgtataacaa gagcaaaatt accaaaaccg
     1501 taaagatgtc aaatgcacgg cgtggatata gattatcatc cggcatattt ggtgaaacga
     1561 taactgacat gctacgcagt tttttggtag cctcaacacg cgccgactcc agcgacgcga
     1621 ctgtagcctt atagttatcc tcggcaatac ccgcatccac ctccaaagca tgatactgag
     1681 aagctattac attaagttga ttgttattag ttttagccac taacttttgc tcttcagccg
     1741 ctatttgctg ctgcagggcg atcaccatga ctttttgctg atttacctgt gggctgtttt
     1801 cgctcagtgt cgcccgcata gcacgcagag tggcattggc attcgtcaaa ttcgattgca
     1861 atgtggaaat aacctcattg cgtgatatgg cgctttgctg ggcatctagg acattatgtg
     1921 cagactggaa gtccaacata atcttgcgtt tttgttcata agtcttacga gctcgttcga
     1981 gttcttcttg agcaaaggcc acttgctctc gagccatttt gtgcgagagt tcgtttacaa
     2041 atttatcgct ttccgaaata atgacatcca gtgttttttt ggcgaatgct ggatcgaagg
     2101 actgcacttt aatagtcagc agtccattaa cttcattata attggtagta atcatgcgct
     2161 ggtaatactt gagctgatct tcttgtggag catcagtagg caagaaatac caagaatcga
     2221 gccaattccc agcataatgt tgattccatt gcagcttttt ctgcaatatg tccaacatat
     2281 cctgtgaatt aacaaattca tttacatata aagtttccat catcgaggcg ccgccacctc
     2341 ctcctcccaa cagagaagcg atcccaggca attgcactgt actactgcca ctgtctgaac
     2401 tttccgagcg caccacgaca gacgatgtac ttacatagcg atctcttgct aaaaatacaa
     2461 aataaataat aataatcagc atgggaatga tcaccatgcc cgtgaaaagt cgatttttta
     2521 acaatttctt gaatattgac atgctgctct tgaaagcgat ttaagtgaat aacagcctac
     2581 ccagcttcgg tttaaatttt cgttccctgc aaccagagac tcgttaccgt tcgtagctga
     2641 aaaggccgta gtgctgccca cttatcttga cgggcataga taaaattctt gcaaatatct
     2701 ttaaccacaa gactgttttc tctattcaaa gttggttttt gttcgaaatt tcacgagttt
     2761 ataactgatc cacttagaat tgaaaaatgg actgtaggag gaaagtattg actgcgccac
     2821 aatcgttata ctttgctacc ttaattggcc ttttttcaat acttcagaat gaatcagccc
     2881 aaggcttcag tacgttaggt gtcgcactta acgcgaagtc aggctgcaaa tagtttagat
     2941 tggggttgta aaacgggtca ttctgcatca catgggccca acgtttgcgc atatagcgca
     3001 cttccttttt tgcccgtcgt aattgtgctc gagtctcatc cttgccccgt gttatagact
     3061 catggtggta cagtagtgca tgcggcgtcc ataccacccg taatcccatt tgacgaacat
     3121 tcaggcaata atccacatca ttgaacgcca cggttaggtt cgcctcgttc agaccaccca
     3181 atgattggta caccgccttt ttggtcaata gacaagcggc cgtcacggac gacaagtcct
     3241 gcgcgaccaa ggcccggccg ttatagcccg gctcatcggc accaatgcca ctgtgcaagt
     3301 ggttggcgca cccccccggc cccaccgtat cgccagcgtg ctgcaccgtg ccattatcaa
     3361 acagcagctt ggcgcccact gcccccacat cattgcgcag cagcaggcca gccatttcgt
     3421 ccagccaatc aggcgagatg acttccgtat cgttgttgag caggcaaacc agttctcctt
     3481 tggcttctcg cacggccatg ttgttgatct tggagtagtt gaagggccgg tcgaatggta
     3541 gcacccgcac gttaggcaac ttgctcaggg tttgcaagta ctccagggtg gctggatcac
     3601 ggctttggtt gtccaccacc aagatctcga aaacctggta ctgcgtcttc tccaacacgc
     3661 tgctgacaca ccgctgcaga tgatccagct catcccgcgt gggaatgatg atgcttacca
     3721 tcggcgccgg ttgggcaacg gggtaatgca cacgacaggt atcccgacta gtagccacga
     3781 ccgtggcggg aatgcgcaac tgacgaaggt attgcgctac aacatgggat gtatcgtcgg
     3841 catgcacggg ctctggcggc aaatgcaaca ggggcgcagg cgtatggaca accggggtat
     3901 caccggccgt caaagacagc aacatcccat gcaaggtgtc aatatggcta tcggccacgg
     3961 gcgcgacatg caggcaagcc gcatgctggc tgttccaggc aaaggtctcg cctatgtagt
     4021 tggtcgaccg caacaactcg ggtgaccagt ccggcttgaa aatgggaacg tgcaagtcgc
     4081 ctttagggct gacactatcg tggtcggtgt agatgacgct tgcgttcggg cgacgcagcg
     4141 cggaatcggc caggcagtag agcgcctgct cggccagttg cgtaccggct cgcagcacca
     4201 tgcaccacct gtcctggccg gtcgcctgcc gggctgccca ggcacaggac caacggtctg
     4261 ccggcaactc cactacatca aaatgagagt agatctgcgt ggccaggctc tcgcgagttg
     4321 cctggagagc ggcctcgcca ctagcctggg tcaggatagt gatgtgaaac cgcggcggcg
     4381 ttttccagcg agccaggcgg cgggccatga cccttcgatc gtgatcggaa atcgaataga
     4441 actctttatg ccattcgtgg tagtcactgc ggcgcaaggc cgtccgaaaa cgaacggctg
     4501 cgtcatacgc cttgtccaga ttggttaata gcgtcttgac cgtaagtccc accctgccgc
     4561 gtcgcttggc gggatgcttg actatagatc gcgtcacgcg atagatctgc cgaagtcgat
     4621 attccgctgc gcgcaatggc cgacaagaaa gctgctcgac cttgccctgg agcatctgtt
     4681 cagccaggcg catcgaacgt acaaacccgg gaaccttcac cagctcaagc accaagcctt
     4741 tgcgagtcac caggacaggc atggcatggg tttcgccagt gttcagttcc aggatgaggt
     4801 cgaccacatg ggccttggcg tgccccgtca cctcgccgcg caccaagtag gcatggtgtc
     4861 gcgtggcagc cttggacgcc atccaggact cggacaccaa catcacgtcc tctccctgaa
     4921 caacctggcc aaccaatgcc gccagcgcgg cacaccgggc ttggcacgcc attcgatcaa
     4981 ttcgcgcagc acacgctccg gcgtggtgaa ctgcccggtc acccggctca cataagtggg
     5041 atacaaaatc aacgcgcccg ccaccagttc gtccaggtcc agcacgcggg tgcgccggcg
     5101 gcgcaacgat tggcttagat ccgtgtcgtg tgtgaggccc cagcctgcgt agaacggctg
     5161 gccgtaggtc accaccggca ccttgcgcag caaggcttcg aagcccgtca atgaggtcag
     5221 cacatgcact tcgtcgacga tgtccagtaa gtgttgtatg ggcacctggc ccacgatctc
     5281 gtcgcaccag gcggcggccc gctcctcatc ctgccctgcc ctgcgcaggc cggacacgac
     5341 atcggggtgc ggcttataga agatataggc gtcggggttg tcttcgcgca cggcgcgcag
     5401 caaacccata ttgctgcgga tgttggaggc gccgaattgg acggaggcat catcctccac
     5461 ctgccccggc accaggatca cgtgctggcc gttgggtcgc ggcaagccgc gcggcccagg
     5521 cagattgtat ttggtcaggc ccgcggcgca gatggcatgg cgcagcaggg tggcgcgggc
     5581 gatcagctcg gcggaaaatt ccgtgtgcag cagcagctct tccaaccgcg aaggccgcgt
     5641 agcgtcgtag tagatgccca gttcgtcctg cacccaggac aaggggcgcg tgcggtctgc
     5701 gcccagccct accgagcgca gaaagccgtc gctgatacgc acgatgggct ttctgcccag
     5761 actagcgtct tgcgtattgc cccagacagc cacggcatga ttgttcgtaa cgcgcgcgcg
     5821 ccgctgaaag gacacgccgc tgccctggaa gaaggcgcgc gcgtggcgct ttttccagcc
     5881 ggtaaagccc acggcgtcga cttgcgtgtc gaagcgctgg cgcatgcggc gttgcagggc
     5941 cagccaggcc agcagggttt ccacctcgca acgcttgcct gtctcggggt cgacatagcg
     6001 gctgtagtcg atcagggtgg cgtgcaccag ttgttccagg ggcaccggac cgcgccgcag
     6061 cggcatgggc atgtcgtctt tcgtcagccc ccagcccgcg taaaacggca tgccgaacac
     6121 gcgcaccggc acgccccaca gcagggcctc gaagcccagc tgcgaggtca ccgtatacac
     6181 ctgttgcacg cggaacagca ggctggccgg atgcacgtcg gcatccagca ccttgacgcg
     6241 tggcatggca cgcgcggcgg ccaggtcgaa atggccttgc ttgcggccgg ccaccacgtc
     6301 gggggacgtc ttgagcaaga tggtgctgcc ggggtgctcg tccagggcgg atttgagcat
     6361 gcgggcaaag ctgcgctcgt ccgcctggcc gccgcttatc gacgcgtcgc cgcgcgtctg
     6421 gtcgacgacc agcacgcatt gttccggcaa ggactggtac aggtcgcggc tgtggttgta
     6481 ttgggagacc tggccgtcgc accaggcgcg tatcaaggcc tgtgcacgtt cggtccgcct
     6541 ggaaatcaac ggccgcttaa tcaatgcctc caggcgcgag ggctgggcgg cgtccaggta
     6601 caggcccaga tcgtccagca cgatggagag cggcgggctg tcctgcccca ggccgacgga
     6661 gcgcaggaaa ccgtcttcga tgtgatagac gcccaggccg tgctttttgg catagcgctg
     6721 cgcgcgttgc gcactgggcc gcaagcccca ggccagcacg ctgccttgcc cgtccaagcc
     6781 cttggggcgg tattgcaggg cgaagtcgtt cagcagcgcg gccagcgtgc taatgcgctg
     6841 gatgccgggc gcgcaggtat aaagccgcgg ccggcccagc tctgctgcgt aatgagctga
     6901 ggcatgggct tgcgtggcgg aatcatgtcg gcgggtgggt aggtcagtcg aagacatcgg
     6961 cctgagcgaa gggacaggcc tgggcatctt tttcagacaa cagaggttca atgcccggcg
     7021 gccaatcgat gttcagttgc gggtcgctcc acaggatcga gcgttccagc tccttggacc
     7081 aataatcggt ggtcttgtag agaaagtcgg cgctttccga cacaaccatg aaggcatggg
     7141 caaagccttc gggtatccag aactgccgct tgttctcttc cgagagcagc acgcccaccc
     7201 atgtgccgaa ggtgggcgaa ctgcggcgga tgtccaccgc cacgtcatac acctcgccgc
     7261 gcaccacgcg caccagcttg ccctgcgggt gcggcggcat ctggtaatgc aggccgcgca
     7321 gcacgccgcg cgacgagcgg gaatggttgt cctgcacaaa atcgactttg cggccgatgg
     7381 cctcgtcaaa gcgcttttgg ttgaaacttt cgtagaaaaa gccgcgtgcg tcgccgaaga
     7441 cggagggttc gaggatcttg acgtcggcta tggcggtgtc gacgaccttc acacccgcac
     7501 ctcggccagg atgtttttca ggtacttgcc gtagccgttt ttcttcatgg ggtctgccag
     7561 tttatccagt tggatggcgt caatgtagcc caggcgatag gcgatttctt cggggcaggc
     7621 cactttcagg ccctggcgcg cctcgatggt ggcgatgaac tggccggcct ccatcatgga
     7681 gtcgtgcgtg ccggtgtcca gccacgcata gccgcggccc atggtcacca cgtccagcag
     7741 gctcttctcc atgtagatct ggttgaggtc ggtgatttcc agctcgccgc gcggcgaagg
     7801 cttgatggac ttggcatagt ccaccacgcg ggcatcgtag aaatacaggc cggtgacggc
     7861 gtagttcgaa cgcggggcgg tgggcttttc ttcgatgctc agcgcgcggc cgtatttatc
     7921 gaactccacc acgccgtagc gttcggggtc gtgcacgtga taggcgaaca cggtggcgcc
     7981 tgcctcgcgc ttgccggcct gttccagcag gttggcgatg tcgtggccgt agaagatgtt
     8041 gtcgcccagg atcagggccg aaggcgcgcc gtcgaggaag cgctcgccga tcagaaaggc
     8101 ttgcgccagg ccgtcggggc tgtcttgcac ggcatactgc aggttgatgc cccactcgct
     8161 gccgtcgccc agcagttgct ggaagcgcgg ggtgtcctgc ggggtggaga tgatgaggat
     8221 gtcttgcagg cccgccagca tcagcacgct gagcgggtag tagatcatgg gcttgtcgta
     8281 gacgggcaac aattgcttgg acatggccaa agttgcagga tacagccgag tacccgaacc
     8341 gccagccagg atgattcctt tgcgcttttt cacttgcagg ctccgtgcgt caaacatcaa
     8401 acgtgctcga tgggggtagc ctagccaaac cggcttgaaa gttccggtcg agtctttctt
     8461 tcacttgtta ccaagaatgt caataaaaca aaaggaaggt tgaagcttaa acgttcgtaa
     8521 agccatgaaa atttgaggat ccggtaacaa ctgcctccac aaaatggata agctttggcc
     8581 tacccagttt tggctagaag cttgcatgtt tcgcttactc ttccgacagg ccgcacctga
     8641 aagcgctcac ctgagccatc cccgtcctgc accgcagcaa tggctggtgc tgcagcaagg
     8701 ggccaatccg tccacggatt actacgtgcg cccgcacttg gaagccacgg gtttacccat
     8761 tagctacaaa aatctggacg ctatccccga tgtgcaagcg cacattttgc cggggactgg
     8821 cgttgttatc gttcgttacc taaatgtcca atgggcccgg gcattgcgcg cgcaacgatt
     8881 gcatttgagc cgcgtggcct acttcatgga cgacgagctg ctcaggccga cgacctgggc
     8941 gggactgccc aagccttatg ccaagaaatt gcgggcctat tgcctggccc gcgcgggaga
     9001 tatccgcggg ctggccaccg actactggtt ttccacccgg gcgctgcagc gcagccatgg
     9061 ccacctgccc tccgaggtgc tgccgccccg ccccctggcg caagaccgcg ccgcgccgcg
     9121 catctggcgc gcttcccaca ccagcgaggc gccgctgctg gtgttctacc acggctcggc
     9181 cgcccaccag gccgagatcg cctggctgcg ccccgtcatg gccgaggtgc tggcgcgctg
     9241 ccccaatgtg catttcgaga tcatcggcga ccacgaggtc aacaagcgct ttcgcggcct
     9301 gccgcgcacg cgcgtcgtgc atcccatggg ctggcccagc tatctcaacc actgccgcac
     9361 gcttgccggg catatcggcc tggcgccgct gctggacacg cccttcaacg caggccgcgc
     9421 ccatgtaaag gccttcgata tctcccgctg cggcgcgatg ggggtctatt cggcccaggg
     9481 cccgtacggc caggtcatcc gcgatggcga aaacgggctg ctgctggata acctgcccga
     9541 gctatgggtg gaggccataa cctcgctggt caacgacacg cccaggctgg atacgctgcg
     9601 gcgcgcggcg atggcgttgc tgggataggc aaaaaccagt accgcttgcc acccctggcg
     9661 aaatcctgct gcatgaatgg ctggaaccca tgggcatttc tcaatacgcc ctggccaagg
     9721 ctatcgatgt gcccccccgc cgcatcaacg aaatcgtcaa gggcgagcgc gccatctccg
     9781 ccgataccgc ggtgcggctc ggcatcttct ttggcaccga tgcacaaagc tggatgaacc
     9841 tgcaaaccca ttacgacacc gagacagcgc gggaaaaaat cggccatgac aaactggccg
     9901 aactcgagcg ccacgccctg gcagcgtgat accgcctgga atcaatcgta ccccagccgc
     9961 cgcaagaacc accccgacac ggccgggtgg atgccggcca gcaaaaagca ccccaggttc
    10021 aacggaaacg gaaagctgat gcgggcccgg ttggcctgca agcccttgcg gatatacgtc
    10081 gcggcccgcg cggccgtcca caaaaaaggc ttggggcccg gcatggcgaa gcacatcttc
    10141 gattcgacat agccgggcat gatcacgttg accttgatgc cctgccccgc cagcccgtcg
    10201 cgcagggctt cgccgaacac cttgatggcg gccttgctgg cgcagtagct gggggtttcg
    10261 ggcaggccgc gccaggcggc cagcgagctc accagggcga tctggccgct gccgcgcgcg
    10321 cgcatggcgg gcacgaccgc gtgcaccgtg gccatggccg ccttgacgtt gacgtcgaag
    10381 agccggtgca tgtcttccca gctttcgtcc tgcgtgccgg cctcggcgtg gatgttgacc
    10441 ccggcgttga ccagcaccag gtcgggcggc gcggatgcgc cgacctcgcc cagccattgc
    10501 atcagggctt ggtggtcgcg cgcgtcgatg ccggcggtga tcacgcgcgc gcccagcgcg
    10561 gcgcattgcc cggccacctc ggccagctgg cccgcatcgc gggcatgcag gatgagggtg
    10621 gccgtgccgg cccgggcgta ttcgagcgcc agggccgagc ctatgccgcc ggcggcgccg
    10681 gtgatcaata cacaatcggg aacggccgtt ctcattgtta tggcaagcct tgtagagtga
    10741 aaaagccccc ttgcgggggc tgttgcaggg tgcggcgcgc gattattgca gcaggctgcg
    10801 cagcatccag gcgtttttct cgtggatgtc caggcgctgc gtcagcaggt cggccgtggg
    10861 ctggtcgttg gccgtgtcgg ccttggtgaa ggcggcgcgg gcggtcttgg cgacggcctc
    10921 gttggcggcg accaggaagc gcaccatgtc catcgcctcg ggcacggcct tgggctcttc
    10981 gatggagctc agcttggcgt attcgcggta ggtgccgggc gcgtagtggc ccagcgcgcg
    11041 gatgcgctcg gcgatgctgt cgagcgcgcc ccactcttcg gtgtactggt ccatgaacat
    11101 ggtgtgcagc gtgttgaaga gcggacccgt gacgttccag tggaagttgt gggtggacag
    11161 gtacagggta taggaatccg ccagcagctt ggaaagctcg gcagcgacgg cggctcggtc
    11221 tttttcggag ataccgatgt tgatgcgagg gggcaggctg gttttgctct tggccatgtc
    11281 gataggctcc tggacttttt aggtgtttga aagaatacca ctgccctatg cggcacaggc
    11341 acggggcggg cggcaggatc aggccgagac gggttcgtcg ctcagcatcg tcacgtgggg
    11401 caggccgcag gcatagacgg cctgggacag ggcctcgatc gccgccatgc gcgggaagct
    11461 gcggcgccag gccagcacca cgcgccgatc gggcacgtgg ccggcaaagg gcacgtagcg
    11521 cagcaggctt ttctcgggcc ggatttcggg cacggcggtc atgggcagca cggtcacgcc
    11581 gatgccggcc gccaccatgt gccggatggt ctccagcgac gagccctcga aggtgcgctg
    11641 gatgccgtcg ctggcggccg aaaagcgcga cagctcgggg cagacctcca gcacctggtc
    11701 gcgaaagcaa tggccggtgc ccagcagcag catgttctgc ttcttgaggt cttgcgcgtc
    11761 gatggctttg agcttggcca gttcgtgatc ctgcggcacg gccacgatga agggctcgtc
    11821 gtacagcggc tgcatgacca ggcccgcttc gggcaagggc aaggccatga tggcgcagtc
    11881 gatctcgccc tggcgcagca gctccagcag gcgcacggtg aagttttcct gcagcaccag
    11941 cggcatctgc ggcgtgcgct cgatctgcgc gggcaccagg tacggcaaca gataggggcc
    12001 gacggtatgg atgacgccca cgcgcaaggg gccggccagc gggtcgtggc cctggcgggc
    12061 gatctccttg atgctggcgc tttcttccag caccttctgc gcctgggcca cgatgcgctg
    12121 gcccatgggc gtgacgccca cctcggtgcc gccgcgctcg aacagcgtga cgcccagctc
    12181 gtcttccagc ttgcggatgg ccaccgacaa ggtgggctgg ctgacgaagc atgcctcggc
    12241 ggcccgcccg aagtggcgtt cccgcgcgac agcgacgatg tacttcaact cggtaagcgt
    12301 catggttgca tccgctgcgt gaggttgacg gttggattct tcatatcggt taagtgcgca
    12361 ggaagtcttc gcggccgcgc atccagcggt caaggtgggc ctgtacggcc ttgggatcgt
    12421 gctggcgcag ccaggccgcg gcttcgcggg cctgttcggc gatggcggca tcgagttcga
    12481 tgtcggcaaa gcgcagcaag gccaggcccg actggcgcgt gcccaggaac tcgcccgggc
    12541 cgcgctgggc caggtcgcgg cgcgcgatct cgaagccgtc gttggtttcg aacatggcgc
    12601 gcaggcgttc gcgcgccact tgcgacaagg gatgctggta aagcagcacg cacaccgatt
    12661 cggccgtgcc ccgccccacc cggccgcgca actggtgcag ctgcgccagg ccgaagcgtt
    12721 cggcgtgctc gatcaccatg agcgaggcat tgggcacgtc cacgcccact tcgatgacgg
    12781 tggtggccac cagcaggtcg atctcgccct ggcggaaggc ctgcatgacg gcagccttct
    12841 cggcctgcgg caagcggccg tgcaccaggc cgatgcgcag gtcgggcaga tcggcgcgca
    12901 tggcgtcgaa cgtatcgacc gcggtctgca gctgcagggc ttcgctttcc tcgaccagcg
    12961 ggcataccca gtaggcctgc tgcccctggc gcgcggcctg ggcgacgtgc atgagcactt
    13021 cttcgcggcg ggcatcggcc gccagcttgg tgagcaccgg cgtgcggccc ggcggcagtt
    13081 cgtcgatgac ggacacgtcc aggtcggcga agaaggtcat ggccagggtg cggggtatgg
    13141 gcgtggcgct catgtgcagc tggtgcggcg caagcgcgcc ctcctcgccc ttgcggctga
    13201 gcgccaggcg ctggcccacg ccgaagcgat gctgttcgtc gacgatggac aggcccagct
    13261 tccggaagct cacgtggtcc tgcaccaggg cctgcgtgcc cacgaccagt tgcacggcgc
    13321 cgccggagac ggccgcggcc gtctcgcgcc gcgccttggc cggcaggctg ccgctcagcc
    13381 aggccacttg cacgcccagg ggctccagcc aggccgacag tttgcggtag tgctgctcgg
    13441 ccaggatttc ggtgggcgcc atcaaggcca cttgcgcgcc gttggcgatg acctgcgccg
    13501 cggccatggc ggccaccacg gtcttgccgc tgcccacgtc gccctgcagc aggcggtgca
    13561 tggggtagcc gaggcccagg tcttgcgcga tctcggcgga cacgcgctgc tgcgcctgcg
    13621 tgagccggaa gggcaacgag tcatgcagcc tttgcaccag gttgccgggt tgcgccggcg
    13681 cgggcatgga caaggcctgc ttgctgcgcc gccccgcccg ggccgccgcc agcgacaact
    13741 gctgcgccag caactcgtcg aacttgatgc gccgccaggc cggatgggcg cgttcgatca
    13801 ggtcggcctc ggccacgccg ggcggcggcg catgcagcag gcggatggcc ggctcgaagg
    13861 cttccaggcc gtagcgctgc cgcacgctgt cgggcaaggt gtcggacagg tcggtctgca
    13921 gcagcgcctg gtcgatggcc ttgcgcagcg tgggttgcgg caggccgtcg gtgctggggt
    13981 agacgggcgt gagcgcctgc ggcagcggtg tgtcggcatg ggtcatgcgc gggtgcacca
    14041 tctccttgcc ccacaggccg ccgcgcacct cgccgcgcgc gcgcaggcgg cgccccgcgg
    14101 taagttgctt atgctggctg ccgtagaagt tcagccagcg cagctgcagc tcgcccgtgt
    14161 cgtcggcgat cacggccgtg agctggcggc gcgggcgata gaggacttcc gaacgcagga
    14221 tgtcgccctc gacctgggca ggaaagcccg gccgcaggcg cgcgatgggc gtgatgcgcg
    14281 tttcgtcttc atagcgcagc ggcaggtgca ggatgaagtc ctgcgggtgc cgcaggccca
    14341 gcgcatggaa tttgcgctcg atggcgctca accccgcttc ggctgcggga ggcttgcgag
    14401 agactgccga tgaaggcgcc atgcgcgaag tgccgtgcgt gccgtacgcg gccggatcgc
    14461 ctggtcagat caccaggacg gcttcgactt cgaactgcgc gcccttgggc aggctggcca
    14521 cgcccaccgt cgaccgggcc ggaaaaggct ggggaatgat ctcggccatg atggcgttgg
    14581 ccagggcgaa cttgcccagg tcggtcagga acagggtgag cttgacgatg ccgtccagcg
    14641 tgccgccggc ggcgtcgacc acggccgtga ggttggcgaa ggcctggcgc acctgggcct
    14701 cgaagctttc ggccaccagc tcgcccgtgg cgggctccag gccgatctgg cccgacaggt
    14761 acaccgtctt gctgcccgtg gccgccacgg cctgggaata cggccccact gcggccggcg
    14821 ctgcgtcggt atggatgatt tgcttgctca tgatggtctt cctgcagggt ggcgcctgtc
    14881 gggaggcagg gcacatttgc atagactatc agaaattatg gacaatagcc tacgcatggg
    14941 agtgatggat gcgtccatcc cggcttgaat tctgactttg cgcttataga ctatccggca
    15001 cggatttttt atagtattgc cagggatcat caatgagccg aaccatgctc gccttcgaca
    15061 ccttcaagta tttccatgcg ctgaccaaag ccggggttac ccaagccgag gccgaggcgc
    15121 acacgcatgc cttggtcgag gcgatcaatg ccaatttgca gaacctcgcc accaaggatg
    15181 acctgaagcc attcgccacc aaggatgatc tgaaggtgtt cgccaccaag gacgacctga
    15241 agctattcgc caccaaggat gacctgaagc cgttcgccac caaggacgac ttgaagttat
    15301 tcgccaccaa ggatgatctg aagccgttcg ccaccaagga cgacttgaaa caattggaag
    15361 atcgcatgga aggcaagatg cagcagttcg ccacccgagt tcaactcgaa gaactccgcg
    15421 gcgaggtccg ggcctccgaa ttgcgcatcc aggcccagat tgcgccgatg cgctggatgc
    15481 tcgctgcaac gggcactgcc gtgctggcca tgttcgtcaa cacgtttgca agcccgctgt
    15541 tcaagatgct gcactgacgc tggcgttcac cgccggccgt gcacgcggta ctgctcgaac
    15601 aggcacacgg cagcggccgc ggcgacattg agcgattcca ccgcctcggc atcatgtgga
    15661 atgcgcacgc gcagcgccgc cgcctgcagg agtccgggcg ccacgccctg cccttcatgg
    15721 ccgaatgccc aggcgcaatg ccgcggcagg gggtgggcat agagatcgac cgcgccgtcg
    15781 agcgcggtgg cgaccagcgg cacgtccagg cgcggcaaca aggccagcag gtcctggtgt
    15841 tcgtggatgg cgagcgcgaa gtgcgcgccc tggccgctgc gcaccacctt gggcgaccag
    15901 gcggccgcgg tgccggtgga caggaaaacg cgctttattc cagccgccgc gcaagtgcgc
    15961 aacagcgtgc cgacattgcc cggatcctgg atgcggtcga agagcacgca gttttcggct
    16021 aggcggacag gcggctcggg ctcgggcggg cgcaccagga acagcacgcc ctgcccgctt
    16081 tccacgctgg ccagatcgcg catcaggcgc ggctccaggg cgatgcaggc cgaatcgggc
    16141 agttgcccgg ccagcgcatg cagctcggga tgctgcagtt tttcgctgtc gaacacggcg
    16201 tattcgggcg cgccgtagcg ggccagccag gcctggcaca ggtgcacgcc gtcgagcagc
    16261 acgggttcgc cgtgccggcc cgcggtcgtg gccagcttgc gcagtgtctt gacgagcgga
    16321 ttgtcgcggg aggtgatgag tttcatggcc gtatggctcg tggccgcttc atggctgcag
    16381 caggactctc accggcgcga agctgcggcg gtgctcgggg cacgggccgt gctcgcgcag
    16441 caggctcagg tgctgggccg tgccgtagcc cttgtgcttg tcgaaagcgt actgcgggta
    16501 gagcgcgtgc aggcgcatca gatcggcgtc gcgcgcggtc ttggccagga tggatgccgc
    16561 cgaaatggcc ggcaccttgg cgtcgccctt gatgatggtt tgcaccgcgc agcgcaggcg
    16621 cggcggctgg ttgccgtcga ccagggccag tcgcggggcc acggacaggc cttccacggc
    16681 gcgctgcatg gccagcaggg tggcgcgcag gatgttgagc tggtcgattt cgtcgacgct
    16741 ggcgtgggcg atgcaccagg ccagggactg ggccttgatg aggccggcca gtttttcgcg
    16801 gcgggcggcg ctgagcacct tggaatcggc caggccggcg atgggccggg ccgggtcgag
    16861 gatgacggcg gcggcgtaga cggcgcctgc caatgggccg cggccggctt cgtccacgcc
    16921 tgcggtggtc gaggcgtcga tgttggcttc ggtgctgaac aaattggttt gcatgcttag
    16981 aggttcttga gccgccgagg ctgccctgcg gaattccgat gctcggcgct gatggcgcct
    17041 gcgcttcgga ttcctccggg gtcctcggct tgcgtcaggt cttatacgcg gccatccgct
    17101 acggcggcga tggcttgggc ggccagcgta ggggtatcgc gcagcagctc ctggtgcatc
    17161 gcggtaaagc gtgtctccag gctcgcggcc agcgcatcgt cggtcaaggc cgtccaggtc
    17221 gcggcttcca gcgcctgcgg cgtggcgtcg tcctgcaata gctcgggcac ggcggaatcg
    17281 cgcagcagga tattgggcag gccgacccag ggcagatagg gacgttcctg gcccgatttc
    17341 caggccatga tgcggcgcat ccagggcgag agcacgtagg agatgaccat gggccgcttg
    17401 tagagcgcgg tttccagcgt gg
//
LOCUS       DASWPO010000126         9025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1455378, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000126 DASWPO010000000
VERSION     DASWPO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455378"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /gene="aceF"
                     /locus_tag="VGC69_07265"
     CDS             <1..1432
                     /gene="aceF"
                     /locus_tag="VGC69_07265"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEY0295118.1"
                     /translation="AAAPAPAAAPAAAPAPAAAAPVAAAPVPAPAPAASGGLVELVVP
                     DIGDFKEVEVIEVMVAVGDTIKAEQSLITVESDKASMEIPASQGGVVKSLEVKVGDKI
                     SKGALMAVVEAAGAAPAAAPAAAATPAAVPAAAATPAAAPVPAAAAPAPDSAPKAAPA
                     ASAALEDPGLAPGKLPYASPSVRKFARELGVNLSRVTGSGPKGRITADDVRSFVKQAL
                     SSDAATAGGSADGAALGLLPWPKIDFAKFGPIDPKPLSRIKKISGANLHRNWVMIPHV
                     TNNDEADITDLEALRVTLNKENEKSGIKVTMLAFVIKAVVASLKKFPEFNASLDGDNL
                     VLKHYYHIGFAADTPNGLVVPVVRDADRKGILELAQETSELAKKARDGKISPADMQGG
                     CFSISSLGGIGGTHFTPIINAPEVAILGLSRSFQKPVWDGKQFVPRLTLPMSLSYDHR
                     VIDGASAARFNAYLAALLADFRRIVL"
     gene            1443..3218
                     /gene="lpdA"
                     /locus_tag="VGC69_07270"
     CDS             1443..3218
                     /gene="lpdA"
                     /locus_tag="VGC69_07270"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0295119.1"
                     /translation="MSNVQIKVPDIGDFKEVEVIEVLVKAGDNIRAEQSLITVESDKA
                     SMEIPASAGGVVKAVLVKVGDKVAEGSVILEVDAASAPAAASAPTPAPAAAPAPAAAP
                     AASAPAASAPAAASYSGSADLECDVLVLGAGPGGYSAAFRAADLGLNTVLVERYATLG
                     GVCLNVGCIPSKALLHNAAVIDEARALAAHGVSFGEPVVDLDKLRAYKDSVVAKLTGG
                     LASMAKMRKVTVVTGVGEFADPNHLSVKSADGKTRTIRFKSAIIAAGSQSVKLPFLPA
                     DDRIVDSTGALLLKSIPKKMLIIGGGIIGLEMGTVYSTLGARLDVVEMQDGLMQGADR
                     DLVKVWQKFNTSRFDHIMVNTKTVAAEAKPDGIYVTFEGEGAPKEPQRYDLVLQAVGR
                     SPNGKKIGAERAGVAVSDRGFIAVDKQMRTNVPHIYAIGDLVGQPMLAHKAVHEGHVA
                     AEAIAGEKSFFDARVIPGVAFTDPEVAWVGLTEDDAKKQGIKIEKGVFPWAASGRAIA
                     NGRDEGFTKLIFDAETHRIVGGGIVGTHAGDMISEVVLAIEMGADIVDIAKTIHPHPT
                     LGESVGMAAEIAEGVCTDVPPTRRK"
     gene            3288..5282
                     /locus_tag="VGC69_07275"
     CDS             3288..5282
                     /locus_tag="VGC69_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0295120.1"
                     /translation="MPLIQTLPALHEPRARRFAFQLMLASVVAMVTANALGLANPWWA
                     AMAVWMVSQPTRGLLLERSAAQLLGTCLGAAAGTALALIPDLGLRLVGLALWMALACG
                     TGNLMRHQRAYGAVMASLAAVVVVLMSGGMDPARFALMRVVDTVIGIAASVFLIALWS
                     PRGPGEALLARARTAGEEALSLAGSILGATASGRVDSQAREQRLLADLAGIEAASEDA
                     ASGSIEYRRRLRPLRGLLAALLDVMAVTRIVRERAERNQAKPGGHEGLALAQTCGRAL
                     DQYAQGLGDEARRGEAAAQLDACAQAARQQESVLASLLETLQASLAAAHNDYLELASS
                     LPGEVSGHALSSPNWAGAKRTVLRCLLACLAPAALWLLTGDELARYVVLGSCVFISVF
                     AVADDPAVILRQILIGGVGAVAGTVIWRAGLMHVLESGYESLILAVPFMFVAAVIQAN
                     RPTAFIGLALNMIFAVMAQPIQLHPAPAETVALSGLALLGGLVFSLVCFRWLVPMSSA
                     HRRADLRRAMRRELGAISARAGDPRAARHLARLRYLALDLLSRAGTPLDMAHGALSAL
                     VLGHTLLRIGSLVHGADVSAAAHRAAQAAMDELRRGGLAPGYLSASLSRAAMLVESGM
                     VMMPGSILPMQELSQALRDAAACIAWQPAFLVGISRTSGE"
     gene            5442..6260
                     /locus_tag="VGC69_07280"
     CDS             5442..6260
                     /locus_tag="VGC69_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295121.1"
                     /translation="MMFASKMKRSLACAAAAVCAWSMLAGSAAAAPVPTQEPGMLKVA
                     MAATYPPFEYFDGDKIVGSDVDFGHILARHLGTDVSFQDAKFATLIMGLNAHHYDVVI
                     SGMYMTPERLAQARAVPYARTSSAILATAASGLAPKQPEDLCGLRVGIVTGTVYLPKL
                     HDLSESYCKKNGKGDINISEYASTAEAFQAMLSNNVQVGMQVANTAHALADKSQGRVV
                     VTSTDIIYPQTIGIYVEKDNAALFDVVSKTMAEVVKTPEYAEMLKKSSLFPPEQ"
     gene            6321..7871
                     /locus_tag="VGC69_07285"
     CDS             6321..7871
                     /locus_tag="VGC69_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HEY0295122.1"
                     /translation="MRFDFAYMFSMFAMPAVWRAALVVVELSALSWLSSVVLGFGLAW
                     ARMSPSWWLARPAAIYIWFFRSVPLIVLIIFVYNLPQLFPATGVALSHPFFSGFVALL
                     LTETAYMAELHRGGLMSVGKGQYEAATALGLRPVGIQRLVVIPQALRIALPSLVNQYI
                     ACVKLTALVSVISLRELLLLAEQLYAQNLLVMESLLAITVFYVGIVTFFSWIMTAIER
                     RLDLSRRKAETLPADRLAAIRASLPAAAAGQGAGRQQADVSALLLNGLRKCYGSHEVL
                     KGIDLAVKAGEVISIIGPSGSGKTSLIRTINALETIDQGEIILFGQSYILASDPPRNP
                     QVSRGMRRIGMVFQGFNLFPHRTILDNVILAPCYHGAGRAAATSRALALLDKVGLLAH
                     AYKYPHQLSGGQQQRVAIARALAMEPEIMLFDEPTSALDPELVGEVLKVIAQLAQEGM
                     TMLIVTHEMDFAMSISDRVVFMENGVVQLAAAPAEIRERPDAERVRRFVGLPAQAVQD
                     PVVLQTAA"
     gene            complement(7910..8476)
                     /locus_tag="VGC69_07290"
     CDS             complement(7910..8476)
                     /locus_tag="VGC69_07290"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY0295123.1"
                     /translation="MTERHRCAWAVNDPLMRDYHDREWGVPLRDGRALWECLMLEGFQ
                     AGLAWIVVLRKRAAFQEAFAGFDPHAVARFGEADVLRLMANPGIIRARAKIEATIRGA
                     QIYCDMEERGERFSDFCWSFTAGKTLQGDGHGPVANTPLSERISKEFKRRGFKFVGPT
                     IVYAWMQAVGIVNDHSADCFCRKASAPA"
     gene            8691..>9025
                     /locus_tag="VGC69_07295"
     CDS             8691..>9025
                     /locus_tag="VGC69_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEY0295124.1"
                     /translation="MAMMNKDNAGVRMSLTAVAAALALAGCGGSDDSSPSANNSTTPS
                     YSGVVVANGYTPGSTTGNPTLKAGYYSGATVFVDTNGNGVLDGNEASTKTDCNGHFTL
                     QTNATGQLVA"
BASE COUNT         1420 a         3123 c         2985 g         1497 t
ORIGIN      
        1 cgcggccgcg cccgccccgg ctgccgcacc cgccgcggcg ccggcgcctg ccgcggcggc
       61 gccagtggcc gccgcgcccg tgccggcgcc cgcgcccgcc gccagcggcg gcctggtcga
      121 gctggtcgtg cccgatatcg gcgacttcaa ggaagtcgag gtcatcgaag tgatggtggc
      181 cgtcggcgat accatcaagg ccgaacaaag cctgatcacc gtcgaatccg acaaggcctc
      241 gatggagatc cccgcctcgc aaggcggcgt ggtcaagtcg ctcgaggtca aggtaggcga
      301 caagatctcc aagggcgcgc tgatggccgt cgtcgaggct gccggtgccg cgccggcggc
      361 ggcgccggcc gccgcagcaa cgccggccgc ggtgccggcc gccgcagcaa cgccggccgc
      421 ggcgcctgtc ccggctgccg ccgcgccggc gcccgattcc gcccccaagg ccgcgccggc
      481 cgcctcggcc gcgttggaag atccgggcct ggcgcccggc aagctgccgt atgcctcgcc
      541 ctcggtgcgc aagttcgcgc gcgagctggg cgtcaacctg agccgcgtca ccggcagcgg
      601 ccccaagggc cgcatcacgg ccgacgatgt gcgcagcttc gtcaagcagg cgctgtcctc
      661 ggatgccgcc acggccggcg gttcggccga tggcgccgcg ctgggcctct tgccctggcc
      721 caagatcgat ttcgccaagt tcggccccat cgaccccaag ccgctgtcgc gcatcaagaa
      781 gatctcgggc gccaacctgc accgcaactg ggtcatgatc ccgcacgtca ccaacaacga
      841 cgaagcggac atcaccgacc tggaagccct gcgcgtcacg ctcaacaaag agaacgagaa
      901 gtcgggcatc aaggtcacca tgctggcctt cgtcatcaag gccgtggtcg cgtccctgaa
      961 gaagttcccc gagttcaacg cctcgctcga cggcgacaat ctcgtgctca agcactacta
     1021 ccacatcggc ttcgcggccg acacgcccaa tgggctggtg gtgccggtgg tgcgcgatgc
     1081 cgacaggaag ggcatcctgg aactggccca ggaaacgtcc gagctcgcca agaaggcgcg
     1141 cgacggcaag atctcgccgg ccgacatgca aggcggctgc ttctcgatct cgtcgctggg
     1201 cggcatcggc ggcacgcact tcacgcccat catcaatgcg cccgaagtgg ccatcctggg
     1261 cctgtcgcgt tccttccaga agccggtctg ggacggcaag caattcgtgc cgcgcctgac
     1321 tctgccgatg tcgctgtcgt acgaccaccg cgtcatcgac ggcgcctcgg cggcgcgctt
     1381 caacgcctac ctggctgcgc tgctggccga cttccgccgc atcgtgctgt aagggaaccg
     1441 ccatgagcaa tgtgcaaatc aaggtgcccg atatcggcga cttcaaggaa gtcgaggtca
     1501 tcgaggtgct ggtcaaggcc ggcgacaaca tccgcgccga acagagcctg atcacggtcg
     1561 agtccgacaa ggcctcgatg gaaatccctg cttctgcggg cggggtggtc aaggccgtgc
     1621 tggtcaaggt gggcgacaag gtcgccgaag gcagcgtgat cctcgaggtc gatgccgcgt
     1681 cggccccggc ggccgcctcc gcgccgacgc ctgccccggc tgccgcgcct gccccggccg
     1741 cggcgcccgc tgcctcggcc cccgctgcct cggcgcctgc cgcggccagc tactcgggtt
     1801 cggccgacct cgaatgcgac gtgctggtgc tgggcgccgg ccccggcggc tattcggccg
     1861 ccttccgcgc ggccgacctg ggcctgaaca cggtgctggt cgagcgctat gccacgctgg
     1921 gcggcgtttg cctgaacgtg ggctgcatcc cgtccaaggc gctgctgcac aacgccgccg
     1981 tcatcgacga ggcgcgggcc ctggccgcgc atggtgtgag cttcggcgag cccgtggtcg
     2041 acctggacaa gctgcgcgcc tacaaggaca gcgtggtggc caagctcacc ggcggcctgg
     2101 ccagcatggc caagatgcgc aaggtcacgg tggtcacggg cgtgggcgag ttcgccgacc
     2161 cgaaccacct gtcggtgaag tctgccgacg gcaaaacccg gaccatccgc ttcaagagcg
     2221 ccatcatcgc cgccggcagc cagtcggtga agctgccttt cctgcctgcc gacgaccgca
     2281 tcgtcgattc caccggcgcg ctgctgctca agagcatccc caagaagatg ctcatcatcg
     2341 gcggcggcat catcggcctg gaaatgggca cggtgtattc cacgctgggc gcccgcctgg
     2401 acgtggtcga gatgcaggac ggcctgatgc agggcgccga ccgcgacctg gtcaaggtct
     2461 ggcagaagtt caacaccagc cgcttcgacc acatcatggt caacaccaag accgtggcgg
     2521 ccgaagccaa gcccgacggc atctacgtca ccttcgaagg cgagggcgcg ccgaaggagc
     2581 cgcagcgcta cgacctggtt ctccaggccg tggggcgcag ccccaacggc aagaagatcg
     2641 gcgccgagcg cgccggcgtg gccgtgtccg atcgcggctt catcgcggtg gacaagcaga
     2701 tgcgcaccaa cgtgccgcac atctacgcca tcggcgacct cgtgggccag cccatgctgg
     2761 cgcacaaggc cgtgcatgaa ggccacgtgg cggccgaagc cattgccggc gagaagtcct
     2821 tcttcgatgc gcgcgtcatc ccgggcgtgg ccttcaccga tcccgaagtg gcctgggtgg
     2881 gcctgaccga ggacgatgcc aagaagcagg gcatcaagat cgagaagggc gtgtttccct
     2941 gggcggcatc gggccgcgcg atcgccaacg gccgcgacga aggcttcacc aagctgatct
     3001 tcgacgccga aacgcatcgc atcgtcggcg gcggcatcgt cggcacccat gccggcgaca
     3061 tgatcagcga agtcgtgctg gccatcgaga tgggggccga catcgtcgac atcgccaaga
     3121 ccatccaccc gcaccccacc ctgggcgaat cggtcggcat ggccgccgaa atcgccgagg
     3181 gcgtgtgtac cgatgtgccg cccacgcggc gcaaatgacg cgagcaggca gtacaaccga
     3241 aagcggcccg gcgggccgct ttctactttg ttggaaaaga aaaacagatg ccgctgatac
     3301 aaacgctgcc cgccctgcac gaaccgcgcg cgcggcgctt tgccttccag ctgatgctgg
     3361 cttcggtggt ggccatggtc accgccaatg cgctgggcct ggccaatccc tggtgggcgg
     3421 ccatggctgt gtggatggtc agccagccca cgcggggcct gctgctcgag cgcagcgccg
     3481 cgcagctgct ggggacctgc ctgggggcgg cggccggtac ggccctggcg ctgattccgg
     3541 atctcggcct gcgcctggtc ggcctggcgc tttggatggc gctggcctgc ggcacgggca
     3601 acctgatgcg gcaccagcgc gcctatggcg cggtgatggc gagcctggcc gcggtggtcg
     3661 tggtgctcat gagcggcggc atggatccgg cccgctttgc cctgatgcgc gtcgtcgata
     3721 cggtcatcgg catcgcggcg tcggttttcc tgatcgcctt gtggagccct cgggggccgg
     3781 gcgaagccct gctggcgcgg gcccggacgg caggcgagga ggcgctgtcc ctggcggggt
     3841 cgattctcgg cgccacggct tcggggcggg tcgatagtca ggcgcgcgag cagcgcctgc
     3901 tggccgacct ggcgggcatc gaggccgcgt ccgaggacgc cgcctcgggc tccatcgaat
     3961 accgccgccg cttgcggccc ttgcgcggcc ttctggcggc cctgctggac gtgatggccg
     4021 tcacccgcat cgtgcgcgag cgcgccgagc gcaatcaggc caagccgggc gggcatgagg
     4081 ggctggccct ggcgcagacc tgcggccggg cgctggacca atatgcccag gggttgggag
     4141 acgaggcgcg gcgaggggag gcggccgcgc agctggatgc ctgcgcgcag gcggcgcggc
     4201 agcaggaatc cgtcctggcc agcctcttgg aaacgctgca ggccagcctg gcggccgcgc
     4261 acaatgacta cctggaacta gcttcctccc tgccgggcga ggtatccggc catgccttga
     4321 gctcgcccaa ctgggcaggc gccaagcgca ccgtcctgcg ctgcctgctg gcctgcctgg
     4381 cgccggccgc gctctggctg ctgacaggcg acgagttggc gcgctatgtg gtgctgggct
     4441 cctgcgtatt catttcggtg ttcgccgtgg ccgacgatcc cgccgtgatc ctgcggcaga
     4501 tcctgatcgg cggcgtgggc gccgtggcgg gcaccgtgat ctggcgcgcc gggctgatgc
     4561 atgtgctgga aagcggctat gagtccctga tcctggccgt gcccttcatg ttcgtggccg
     4621 ccgtgatcca ggccaaccgg cccaccgcct tcatcggcct ggcgctgaac atgatcttcg
     4681 ccgtcatggc ccagcccatc cagctgcacc cggcgccggc cgagaccgtg gccttgtccg
     4741 gcctggccct gttgggcggc ctggtgttca gcctggtgtg ctttcgctgg ctcgtgccca
     4801 tgagcagcgc gcatcgccgc gccgacctgc gccgcgccat gcgccgggaa ctcggcgcca
     4861 tcagcgcccg ggccggcgat ccgcgcgcgg cccggcatct ggcacggctg cggtatctcg
     4921 cgctggacct gttgtcgcgg gccggcacgc ccctggacat ggcgcatggc gcgctgtcgg
     4981 ccctggtgct gggccatacc ctgctgcgca tcggcagcct ggtgcatggg gcggacgtga
     5041 gcgccgcggc gcaccgggcc gcccaggccg ccatggacga gctgcgccgc ggcggcctgg
     5101 cgccgggcta cctgagcgcc agcctgtcgc gggccgccat gctggtcgag tccggcatgg
     5161 tcatgatgcc gggctccatc ctgcccatgc aggaactgag ccaggccctg cgcgatgccg
     5221 cggcctgcat cgcctggcag ccggccttcc tggtcgggat aagccgcaca tcaggagaat
     5281 gatttggcgt tcatcgagaa accgtctgac ttaggggtgt ccataggttg acagcatttc
     5341 cagggtaggt cattctctgc tttacgtata ctttatttgt taattcgtat actaacagtg
     5401 tgggtttgaa tcttttgccc ccttatcgtg aggaacagga aatgatgttt gcgtccaaga
     5461 tgaagcgcag cctggcctgc gccgcggcag cggtctgtgc ctggtcgatg ctggccggca
     5521 gcgcggccgc cgcccccgtg cccacccagg agcccggcat gctcaaggtg gccatggccg
     5581 ccacctatcc cccgttcgaa tacttcgacg gcgacaagat cgtcggctcc gacgtcgatt
     5641 tcggccacat cctggccagg cacctgggca cggacgtcag cttccaggac gccaagttcg
     5701 ccaccctgat catgggcctg aacgcccacc actacgacgt cgtcatctcc ggcatgtaca
     5761 tgacgccgga acgcctggcc caggcccgcg ccgtgccgta tgcccgcacc tcgtcggcca
     5821 tcctggccac cgcggccagc ggcctcgcgc ccaagcagcc cgaagacctc tgcggcctgc
     5881 gcgtcggcat cgtgaccggc acggtctacc tgcccaagct gcatgacctg tccgagtcgt
     5941 actgcaagaa aaacggcaag ggcgacatca acatcagcga atacgcctcc acggccgagg
     6001 ccttccaggc catgctgtcg aacaacgtcc aggtcggcat gcaggtggcc aacaccgccc
     6061 atgccctggc cgacaagtcg cagggccgcg tggtggtgac gtccaccgac atcatctacc
     6121 cgcagaccat cggcatctac gtcgaaaagg acaacgccgc cctgttcgac gtggtcagca
     6181 agaccatggc cgaggtggtc aagacgcccg aatacgcgga aatgctcaag aagagcagcc
     6241 tgttcccgcc cgagcagtaa tccggcgccg cgccggcccc cgcaccctgg ttttcagcaa
     6301 cacattttga ttcgcgcctc atgagattcg attttgccta catgttctcc atgttcgcga
     6361 tgcccgccgt ctggcgggcc gcgctcgtcg tggtcgaact cagcgcgttg tcgtggttgt
     6421 cgtcggtggt gctggggttc ggcttggcct gggcgcgcat gtcgccgtcc tggtggctgg
     6481 cgcgcccggc cgccatctat atctggttct tccgcagcgt cccgctgatc gtcctgatca
     6541 tcttcgtcta caacctgccg cagctgtttc cggccacggg cgtggccctg tcgcatccct
     6601 tcttttcggg gttcgtggcc ctgctgctga cggaaaccgc ctacatggcc gagctgcacc
     6661 gcggcggcct gatgtcggtc ggcaaggggc agtacgaggc ggccacggcc ctgggcctgc
     6721 ggcccgtcgg catccagcgc ctggtggtca ttccccaggc cctgcgcatc gccctgccgt
     6781 cgctggtcaa ccagtacatc gcctgcgtca agctcacggc cctggtgtcg gtcatttcgc
     6841 tgcgcgagct gctgctgctg gccgaacagt tgtacgcgca gaacctgctg gtgatggagt
     6901 cgctcctggc catcacggtc ttctacgtcg gcatcgtgac cttcttcagc tggatcatga
     6961 cggccatcga acgccgcctg gacctgtccc gccgcaaagc cgaaaccctg ccggccgacc
     7021 gcctggccgc catccgcgcc agcctgccgg ctgcggcggc ggggcagggg gcgggccgcc
     7081 agcaggccga tgtctcggcg ctgctgctca atgggctgcg caagtgctac ggttcgcacg
     7141 aggtgctcaa gggcatcgac ctcgcggtga aggccggcga ggtgatttcc atcatcggtc
     7201 cgtcgggctc gggcaagacc tcgctcatcc gcaccatcaa cgccctggaa accatcgacc
     7261 agggcgagat cattctcttc ggccagtcgt acatcctggc cagcgatccg ccgcgcaacc
     7321 cgcaggtgag ccgcggcatg cgccgcatcg gcatggtgtt ccagggcttc aacctgttcc
     7381 cgcaccgcac gatcctggac aacgtcatcc tggcgccgtg ctatcacggc gcgggccgcg
     7441 ccgcggccac ctcgcgcgcc ctggcgctgc tcgacaaggt gggcctgctg gcccacgcct
     7501 acaagtatcc gcaccagttg tccggcggcc agcagcagcg cgtggccatt gcccgcgccc
     7561 tggccatgga gccggagatc atgctgttcg acgaacccac ctcggcgctc gatcccgagc
     7621 tggtgggcga ggtgctcaag gtgatcgccc aactggccca ggaaggcatg accatgctca
     7681 tcgtcacgca cgaaatggac ttcgccatgt ccatttccga ccgcgtggtc ttcatggaaa
     7741 acggcgtggt gcaactggcc gccgcgcccg cggaaatccg cgaacggccc gacgccgagc
     7801 gcgtgcgccg cttcgtcggc ctgccggccc aggcggtgca ggatccggtg gtgctgcaga
     7861 cggcggcctg aagcgcccct tgccgtggct ttggacgcgc cggcccggat caggccggcg
     7921 cgctcgcctt tctgcaaaag cagtccgccg aatggtcgtt gacgatgccc accgcctgca
     7981 tccaggcata cacgatggtc gggccgacga acttgaagcc gcggcgcttg aactcttttg
     8041 aaattcgctc ggacagcggg gtgttggcca ccgggccgtg gccgtcgcct tgcagcgtct
     8101 tgcccgcggt gaacgaccag cagaaatcgc tgaagcgctc gccgcgctcc tccatgtcgc
     8161 aataaatctg cgcgccgcgg atggtggcct cgatcttggc gcgggcccgg atgatgcccg
     8221 ggttggccat caggcgcagg acgtcggcct cgccaaagcg cgccacggca tgcgggtcga
     8281 atccggcgaa cgcttcttga aaggcggcgc gtttgcgcag caccacgatc caggccagtc
     8341 ccgcctggaa tccttccagc atcaggcatt cccacagcgc gcggccgtcg cgcagcggca
     8401 cgccccactc gcggtcatgg tagtcgcgca tgagcgggtc attgacggcc caggcgcagc
     8461 gatgtcgttc ggtcatggtg ttctcggttg ttgcatgggc cttcgattat cggttccccg
     8521 tgtgttcaac ttacagtcag tcaactggga gtcagtgaaa cgaagggaaa acttaaagga
     8581 aaagcacaaa ttctgtaaat cagggtcatc acaatgagtc ccggttttat cgcggctgcg
     8641 cggggatctc ttcgcggccg attttttatt acaaaccggg agatttttag atggccatga
     8701 tgaacaagga taatgccggg gtgcgaatgt cgctgaccgc ggtggcggcg gcgcttgcgc
     8761 tggcagggtg cggcggctcg gacgattctt cgccctccgc gaacaattcg accacgccgt
     8821 cttacagcgg tgtcgtcgtc gcgaacggct acacgccggg cagcacgacc ggcaacccga
     8881 ctctcaaggc gggctattac tcgggcgcca ctgtgttcgt cgacaccaac ggcaacggtg
     8941 tgctcgatgg caacgaagcg tccacgaaga ccgactgcaa cggccatttc acgttgcaga
     9001 ccaacgccac gggccagctc gtggc
//
LOCUS       DASWPO010000127         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1991480, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000127 DASWPO010000000
VERSION     DASWPO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991480"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..710
                     /gene="nrdR"
                     /locus_tag="VGC69_07300"
     CDS             231..710
                     /gene="nrdR"
                     /locus_tag="VGC69_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY0295125.1"
                     /translation="MKCPFCGNDDTQVVDSRVSEEGDTIRRRRRCLACDKRFTTYERV
                     ELVMPSVVKRNGSRVEYDPSKLRASLALALRKRSISTAEVDAAVVRIEEKLLASGMRE
                     VPTERIGELVMGELKKLDKVAYVRFASVYKSFEDIGEFIDAIREMQGPPVPGKLRKE"
     gene            complement(715..1674)
                     /locus_tag="VGC69_07305"
     CDS             complement(715..1674)
                     /locus_tag="VGC69_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0295126.1"
                     /translation="MAIPVYFVVTRDIVLLDLAGPGEAFRSANLFAPGTFEPHYCGPA
                     GELETGLAGLYLSRLEPLPDRLPAGALLALSGQVGPGPDWNDPQIRAVVDWLKRDKPA
                     DCTIMSVCAGALLAAAAGLLRGRECTTHHTCLAELARLDPTARVHDNRIFVEDGNLYT
                     SAGITAGIDLALHLVARVCGPQVASATARHMVVYLRRAGQDPALSPWLEGRNHVHPAV
                     HRVQDAILRNPASPWSADSLAGLAHTSPRHLNRLFAEHAGCTPMDYLYRIRLTLAGEL
                     IRETRLSLEKVAERAGFGSAHHLRRVWRRYAPDTPGALRASLH"
     gene            complement(1699..>1799)
                     /locus_tag="VGC69_07310"
     CDS             complement(1699..>1799)
                     /locus_tag="VGC69_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY0295127.1"
                     /translation="RVYSPQEIKDHTELVLMNRFARILPAAQALAG"
BASE COUNT          302 a          615 c          618 g          264 t
ORIGIN      
        1 atcgcgggcc ctttgaggcg ggggcagccc cgcaggggcc gagggcgcct gattgcgcgc
       61 ccgagccccg ccgatcgggc ctgcgatgcc cgccacttcc cgcctgacgg gtcaagaacg
      121 ggtttggaac caacaaacgg ctgccatttg gcagccgttg tcatatcttc actgtccaag
      181 caaacccgtc cccattacaa taatccctac atcggcacag gggattgcgc atgaaatgcc
      241 ctttctgcgg caacgacgat acgcaggtgg tcgacagccg cgtctcggaa gaaggcgaca
      301 ccatccgccg ccgccgccgt tgcctggctt gcgacaagcg cttcaccacc tatgagcgcg
      361 tcgaactggt catgccttcc gtggtcaagc gcaacggcag ccgcgtcgaa tacgacccct
      421 ccaaactgcg cgccagcctt gccctggcct tgcgcaagcg cagcatcagc accgccgaag
      481 tcgatgccgc cgtggtgcgc atcgaagaaa agctcctggc cagcggcatg cgcgaagtgc
      541 ccaccgaacg catcggcgaa ctggtcatgg gcgaactcaa aaagctcgac aaggtggcct
      601 acgtccgctt cgcctcggtc tacaagagct tcgaagacat cggcgagttc atcgacgcca
      661 tccgcgaaat gcagggcccg cccgtgccgg gcaagctgcg caaggaatag gctttcagtg
      721 caggctggcg cgcagggcgc ccggcgtatc cggcgcgtag cgccgccaca cccgccgcag
      781 atgatgcgcc gagccgaagc cggcgcgttc ggccaccttc tccaggctta gccgcgtctc
      841 gcggatcagc tcgccagcca gggtcagccg gatgcgatag agataatcca tgggcgtgca
      901 gcccgcatgt tcggcaaaca ggcggttgag gtggcgcggg ctggtgtgcg ccaacccggc
      961 caggctgtcg gccgaccagg gcgaggccgg gttgcgcagg atggcatcct gcacccggtg
     1021 cacggcgggg tgcacatggt tgcggccttc cagccagggt gacagcgcgg ggtcctggcc
     1081 ggcgcggcgc aggtacacca ccatgtgccg cgcggtcgcg ctggccacct gcgggccgca
     1141 gacgcgcgcc accaggtgca gggccaggtc gatgccggcc gtgatgcccg cgctggtgta
     1201 caggttgccg tcctcgacga agatgcggtt gtcgtgcacg cgggccgtgg ggtcgagccg
     1261 ggccagttcg gccaggcagg tgtggtgggt ggtgcactcc cgcccgcgca acaggccggc
     1321 cgcggccgcc agcagcgcgc cggcgcagac ggacatgatc gtgcagtccg cgggcttgtc
     1381 ccgtttgagc cagtccacca cggcccggat ctgcgggtcg ttccagtccg ggcccggccc
     1441 gacctggccg gacagcgcca gcagcgcgcc cgcgggcagc cgatcgggca agggctccag
     1501 cctgctcaga tagaggccgg ccagcccggt ttccagctcg ccggccgggc cgcagtagtg
     1561 cggttcgaat gtcccggggg cgaacagatt ggccgaacgg aacgcctcgc ccggtccggc
     1621 caggtccagc aggacgatat cgcgcgtgac gacaaagtag accgggatag ccatgggcag
     1681 ccgttcgatg cgcgccgctc agccggccag cgcctgcgcc gcgggcagga tcctggcgaa
     1741 gcggttcatc agcaccagct cggtgtggtc cttgatttcc tgcggcgagt agacgcggc
//
LOCUS       DASWPO010000128         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_474412, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000128 DASWPO010000000
VERSION     DASWPO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474412"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..851)
                     /locus_tag="VGC69_07315"
     CDS             complement(93..851)
                     /locus_tag="VGC69_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY0295128.1"
                     /translation="MSMSTLPRFAAAALACALMAGCAAPQQPDPRDPWEGFNRGVYKF
                     NDTVDHVLIKPVATVYDTVMPDPAQECVHNMFSNLGDVWGGINSLLQGNGVNAINSWG
                     RFLLNTTMGLGGCIDVGSKTGARKIPNDFGTTLGVWGLGQGPYLVLPILGPSTIRDGA
                     GLATAWFANPLWINDVRNIGLRNSLYGLDFVDTRASLLDVTNTIDRTALDPYSFVRDA
                     YLQRRRSLVHDGQPPEPAVSESAVDDTPVSVYHP"
     gene            complement(893..1426)
                     /gene="mlaD"
                     /locus_tag="VGC69_07320"
     CDS             complement(893..1426)
                     /gene="mlaD"
                     /locus_tag="VGC69_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY0295129.1"
                     /translation="MSNSKTDFWVGLFVLLGAAALVFLALRAGNMSSFSFAPTYTLTA
                     SFDNVGGLKVRAPVKSAGVVVGRVAAIAFDNKSFQAAVTLNVETAYQFPKDTSASILT
                     SGLLGEQYVGLTPGAEEENFAAGSKIRYTQSAVVLENLISKFLYNTADKQGSAPGSTP
                     GSASTPAANPPASQPAP"
     gene            complement(1442..2224)
                     /gene="mlaE"
                     /locus_tag="VGC69_07325"
     CDS             complement(1442..2224)
                     /gene="mlaE"
                     /locus_tag="VGC69_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEY0295130.1"
                     /translation="MTSNPIGALGGWVLGLVSGVGYFTRFLGALLARSGIAVRRPRLV
                     SQQVHFIGNYSLLIVAVSGMFVGFVLGLQGYYTLNRYGSEEALGLLVALSLVRELGPV
                     VTALLFAGRAGTSLTAEIGLMKAGEQLAAMEVMAVDPLRRVLVPRFWGGVIAMPILAA
                     VFSMVGILGGWVVGVVLIGVDAGAFWSQMQDGVDVWKDVANGVAKSLVFGVTVTLVAL
                     YEGWHAQPTPEGVARATTRTVVVGSLAVLGLDFLMTALMFGN"
     gene            complement(2221..3069)
                     /locus_tag="VGC69_07330"
     CDS             complement(2221..3069)
                     /locus_tag="VGC69_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295131.1"
                     /translation="MPTDTRNDPELALALDGVALGYGDFTVLSDISMQVQAGQVVAVM
                     GGSGSGKTTLLRAATGQIAAQRGTVKVFGQDVGAQSPEGLRALRQRMGVLFQQGALFT
                     DLNVFENVAFPLREHTRLDEPAILERVLAKLDAVGLRTAAHLAMSETSGGMARRVALA
                     RAVVLEPELILYDEPFAGLDPISLGITARLIRHLSDQLGCASVLITHDVAESFAIADQ
                     VYLVGQGKLAAAGTPKQLEASQDPYVRQFLDGTPDGPVAFRYPDTPAFDAWLARQPGH
                     TAGSKA"
BASE COUNT          536 a          996 c         1155 g          475 t
ORIGIN      
        1 gagcgacgcg acggcgcgca gcggcgcgcg ccaggcggtg cgcaaacgaa aggcggcaaa
       61 ggggcgtgga gaaagcggca tcttcaatgt cctcaggggt gataaacaga aacgggtgtg
      121 tcgtccacgg cgctttccga gacggcgggt tcgggtggct ggccgtcatg gaccaggctg
      181 cggcggcgct gcaggtaggc atcgcgcacg aagctgtagg gatcgagcgc ggtgcggtcg
      241 atggtgttgg tgacgtcgag caggctggcg cgcgtatcga cgaagtccag gccatagagc
      301 gagttgcgca ggccgatatt gcggacgtcg ttgatccaca aggggttggc aaaccaggcg
      361 gtggccaggc ccgcgccgtc gcgtatcgtg ctcgggccca ggatgggcag aaccaggtag
      421 gggccctggc ccaggcccca cacgcccagc gtggtgccga aatcgttggg gatcttgcgg
      481 gcgccggtct tcgagccgac gtcgatgcag ccgcccaggc ccatggtggt gttcagcagg
      541 aagcggcccc agctgttgat ggcgttgacg ccgttgccct gcagcaggct gttgatgccg
      601 ccccagacat cgcccaggtt gctgaacata ttgtgcacgc attcctgcgc cggatcgggc
      661 atgacggtgt catagaccgt ggccacgggc ttgatcagca cgtggtcgac ggtgtcgttg
      721 aacttgtaga cgccgcggtt gaagccttcc cacggatcgc gagggtcggg ttgctggggg
      781 gcggcgcagc cggccatcag ggcgcaggcg agcgctgcgg cggcgaaacg agggagagtg
      841 gacatgctca tgccgaatat tgttggagtg tggcgccggg ggagccgcgc cttcatgggg
      901 cgggctggct ggcgggaggg ttggctgccg gggtgctggc gctgcctggg gtactgcctg
      961 gggcactgcc ttgcttgtcg gccgtgttgt agaggaactt gctgatcagg ttctccagca
     1021 cgaccgcgct ttgtgtatag cggattttgc tgccagcggc aaagttttcc tcttcggcgc
     1081 cgggcgtcag gcccacgtat tgctcgccca gcaggcccga ggtgaggatg gaggccgagg
     1141 tgtccttggg gaactggtag gccgtttcca cgttgagcgt gacggcggcc tggaaacttt
     1201 tgttgtcgaa ggcgatggcg gccacccggc ccaccaccac cccggcgctc ttgactggcg
     1261 cgcgcacctt caggccgccc acattgtcga aggacgcggt cagggtatag gtcggcgcga
     1321 acgagaagct gctcatattg ccggcccgca gcgccaggaa caccagcgcg gcggcgccca
     1381 gcagcacgaa gaggccgacc cagaaatccg ttttcgaatt agacattcga tactccaatc
     1441 attaatttcc gaacatcagg gcggtcatca ggaagtccag gcccagcacg gccagcgaac
     1501 ccaccaccac ggtgcgggtc gtggcgcgcg ccacgccctc gggcgtgggc tgggcatgcc
     1561 agccttcgta cagggccacc agcgtcacgg tgacgccgaa caccaggctc ttggccacgc
     1621 cgttggccac gtctttccag acgtccacgc cgtcctgcat ctgcgaccag aaagcgccgg
     1681 cgtccacgcc gatcagcacc acgcccacca cccagccgcc caggatgccc accatggaga
     1741 acacggcggc gaggatgggc atggcgatca cgccgcccca gaagcgcggc accagcacgc
     1801 ggcgcagcgg gtcgacggcc atgacttcca tggcggccag ctgctcgccg gccttcatca
     1861 ggccgatctc ggcggtgagc gaggtgccgg cgcgcccggc aaagagcagc gcggtgacca
     1921 ccgggcccag ttcgcgcacc agcgacaggg ccaccaacag gcccagggct tcctcggagc
     1981 cgtagcggtt gagcgtgtag tagccctgca ggcccagcac gaagcccacg aacatgcccg
     2041 agacggccac gatgagcagc gagtagttgc cgatgaagtg cacctgctgc gagaccaggc
     2101 ggggccggcg cacggcgatg ccgctgcgcg ccagcagggc gcccaggaac cgggtgaagt
     2161 agccgacgcc gctgacgagg ccgagcaccc agccgcccag cgcgccgatg ggattggaag
     2221 tcatgccttg gatcctgcgg tgtggcccgg ctggcgggcc agccaggcgt cgaaagccgg
     2281 ggtgtcgggg tagcggaagg ccaccgggcc gtcgggcgtg ccgtccagga actggcgcac
     2341 atagggatcc tgcgaggcct cgagctgctt gggcgtgccg gccgcggcca gcttgccctg
     2401 tcctaccagg tacacctggt cggcgatggc gaaggactcg gccacatcgt gcgtgatcag
     2461 caccgaggcg cagcccagct ggtcggacag gtggcggatg aggcgggccg tgatgcccag
     2521 cgagatgggg tccagtccgg cgaaaggctc gtcgtacagg atgagctcgg gctcgagcac
     2581 caccgcacgg gccagcgcca cgcggcgcgc catgccgccg gaggtctccg acatggccag
     2641 gtgggccgcg gtgcgcagcc ccaccgcgtc gagcttggcc agcacgcgtt cgaggatggc
     2701 gggctcgtcg aggcgggtgt gctcgcgcag ggggaaggcg acgttctcga agacgttgag
     2761 atcggtgaag agggcgccct gctggaacag cacgcccatg cgctggcgca gggcgcgcaa
     2821 gccctcgggc gattgcgcgc cgacgtcctg gccgaagacc ttgaccgtgc cgcgctgggc
     2881 ggcgatctgg ccggtggccg cgcgcagcag cgtggtcttg cccgagcccg agccgcccat
     2941 gacggccacg acctggccgg cctgcacctg catggagata tcgctgagca cggtgaaatc
     3001 accatagccc agggccacgc catccagggc gagggcaagt tccgggtcgt tgcgggtgtc
     3061 tgtcggcata ggcaatagag ggagcgggca cgcagcgcgt ccccattgta tccctgtgcc
     3121 tcgggcggat tattccgcca ggccgacaag tctggctggg ga
//
LOCUS       DASWPO010000129        24702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2171034, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000129 DASWPO010000000
VERSION     DASWPO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 24702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24702
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171034"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /gene="eda"
                     /locus_tag="VGC69_07335"
     CDS             complement(<1..647)
                     /gene="eda"
                     /locus_tag="VGC69_07335"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY0295132.1"
                     /translation="MATAMATPAATTVAGLVGIGPVIPVLAFESAEQGENVSRALHAG
                     GVKVLEITLRTEAGLAAIERAAGLAPDIVVGVGTLTSPRQCEQARKAGARFGVSPGLT
                     RDLHQAAQDAGLPLLPGVMTPSDILLALELGYDIVKFFPAQQAGGVPMLNALHGPFPS
                     LKFCPTGGVNAENAPTFLALPNVVCVGGSWLTPKAAVAARDWTEITRLAGAASALA"
     gene            complement(659..2482)
                     /gene="edd"
                     /locus_tag="VGC69_07340"
     CDS             complement(659..2482)
                     /gene="edd"
                     /locus_tag="VGC69_07340"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEY0295133.1"
                     /translation="MPSPLTSLQAVTRRVIERSKATRDAYLARIDAARGRFPVRGALS
                     CSNLAHGFAGLEDRDKIMIKAMREPNIGIVSAYNEMLSAHAPYRDFPEIIKRAARENG
                     GVAQFAGGVPAMCDGVTQGYAGMELSLFSREVIAMGTAVALTHNMFDAALCLGICDKI
                     VPGLMIGALQFGHLPVIFVPSGPMTSGLSNDEKARVRQQYATGQVGRDALLEAESAAY
                     HGHGTCTFYGTANSNQMLMEVMGMHLPGSAFVPPHTPLRDALTAAAARRVLEITAERG
                     QYTPIGHVIDEKAIINGIVALLATGGSTNHTLHLVAMARAAGVVIDWDDFDTLSRIVP
                     LLARVYPNGKADVNHFHAAGGVAFLVRNLLEAGLLHDDVATVMGKGLSRYTHNPELVD
                     GELRWRPAPAQSGDSDVLRPISDPFQPDGGLRLMQGKLGRGVIKVSAVKPEHRMVRAP
                     AVVFDSQDAVKAAFERGELERDFVAVVRFQGARANGMPELHKLTPFLGVLQDKGFKVA
                     LVTDGRMSGASGKVPAVIHLSPEALLAGPIGKVRDGDMLLLDTEAGVLDVEIDAAQWA
                     ARPSAQPAHQADNEVGFGRELFGVFRAAAAPAESGASVFGF"
     gene            2736..3581
                     /locus_tag="VGC69_07345"
     CDS             2736..3581
                     /locus_tag="VGC69_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY0295134.1"
                     /translation="MLLSQVHAMRDQLRPSERKLADYIIEAPREVLDLSMSEAAQRAG
                     VSQPTIARFCRALGFSGFREFKIRLAQAVASQLPAVHRDVRADEPVSGLAAKVLDRNI
                     AALIHVRNTMSVDALAMAIDLLAQASRIEFYGAGGSGISAQDIQHKFFRLGMSSVAYS
                     DPHIFLMSAALLGPGDVVVAVSTSGRTVDIVDAARAALSGGAQVIAITRSHSPLARLA
                     TISLHANVEETTDVLSPMTSRVSHLAIGDILAVGVALQRGPQLLEKISHSKTLLKNRR
                     ISEQS"
     gene            3746..3843
                     /gene="ffs"
                     /locus_tag="VGC69_07350"
     ncRNA           3746..3843
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGC69_07350"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3920..5923
                     /gene="dnaX"
                     /locus_tag="VGC69_07355"
     CDS             3920..5923
                     /gene="dnaX"
                     /locus_tag="VGC69_07355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0295135.1"
                     /translation="MTYLVLARKWRPRSFDTLVGQDHVVRALTHALQTQRLHHAWLFT
                     GTRGVGKTTLSRILAKSLNCETGITPTPCGVCRACTEIDAGRFVDYLELDAASNRGVE
                     EMTQLLEQAVYSPGAGRFKVYMIDEVHMLTGHAFNAMLKTLEEPPPHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPPDAIVGHLQNVLGQENVPFEVPALRLIGQAASGSMRDAL
                     SLTDQAIAYSAGDLTEESVRGMLGTIDQRHLVRLLDSLADGDATGVLAVADELSTRGL
                     SYAGALADLAVLLSKIAIAQRVPGALSAEDPITADTLRLAGRLHADTAQLFYSVAVHS
                     RGELTLAPDEYAGFIMTCLRMLALNGDAGPATIVQAPAVMPAASAPAATSAAAPAPVA
                     APEPKPVAAAPLLAPARPETPRATAAVEPPKAPAAAPAPLPAPTPAKQADTPPWDDTP
                     AAKAMPAAPVAQAVPDEPTAADSPPAWVDEDIPDDAGGYDAGAAFTADPDDEFETLAG
                     AAAPRVDTPRPVRPARAAREPAQARARLTDMTQAAWPELAARLPVSGLAAELARQSAW
                     GGLQGDTVILRVAVKTLAESESRVRLQTVLCEHYGQSLRLEVAVGETGEATAHAVAQQ
                     ERAARQQAAEDAVAADPFVQALVSSFGARVVPGSIRPVDPPAA"
     gene            5989..6315
                     /locus_tag="VGC69_07360"
     CDS             5989..6315
                     /locus_tag="VGC69_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY0295136.1"
                     /translation="MMKGQLAGLMRQAQQMQENMKKAQEALADVMVEGAAGGGLVKVT
                     MNCRNDVKRVAIDPSLLGDDKDMLEDLVAAAFNDALRKAEATSQEKMAGLTAGLPLPP
                     GMKLPF"
     gene            6362..6970
                     /gene="recR"
                     /locus_tag="VGC69_07365"
     CDS             6362..6970
                     /gene="recR"
                     /locus_tag="VGC69_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY0295137.1"
                     /translation="MDPQLPEPEPLRALIEALRRLPGVGVRSARRMAYHLLQHDVQGA
                     DMLGRALSGAVNNLRHCARCNSFTEDELCATCANPRRDPGLLCVVETPADQNMIESSH
                     GYRGLYYVLMGRIAPLEGIGPRELDFQRLLDRACDGVVQEVILATNFTAEGETTAHFI
                     GEALSSRGLKVTRLARGVPAGSELEYVDAGTIAWALMERRST"
     gene            complement(6971..7849)
                     /locus_tag="VGC69_07370"
     CDS             complement(6971..7849)
                     /locus_tag="VGC69_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295138.1"
                     /translation="MMTGSRRQFLAFGAAATLAPWVRKAQAYSCSGQGTPISVGGVTG
                     LDSLADPAHSRTLRQSCRTALYIHEYIWARTKEDLHKAILATFAGAPVDAEFNLMNNP
                     ETWFAQPYKKLFTDVGIQVRYSHVNGFAENRLDDWQHFVSAARRRGIETVAPIFSPNS
                     RQYASAPFSSPKWNYLREGEKAGGAVTTDSPPHYFLAQPEAYRQFVVENLKWGNAAGL
                     HTTFIISPNASGIRFLEETRQAVAYLSKHDALPKAWVVENYNPNALPTYPNTIGTESD
                     SQNVLGVALWLARNTA"
     gene            complement(7893..11333)
                     /locus_tag="VGC69_07375"
     CDS             complement(7893..11333)
                     /locus_tag="VGC69_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0295139.1"
                     /translation="MNAQSQPSDLAARLRALDPAASFLVQAPAGSGKTELLTDRILAL
                     LATVARPEEIVAITFTRKAASEMHARVLGKLREAQGPAPESEHGRRSWALARAALARD
                     AELGWQLLHHPARLAIRTIDSFCASLVRSMPWLSELGGMPEITSDAEAHYAAAAHATL
                     ALADDFEAVRILLEHMDVDVRAAGEAIAGMLAQRDQWMPLLAHGGDRDELESMLSEAI
                     ADDLRAAARALPMGWQAALREPARLAAAELDAAGGANPLAALRDWDDDLACEAAQLPR
                     WQALAHLLLTGAGSLRSARGVNKGLGFPPNSGHKTAFTQWLSGAELDAPRWTGLLHAL
                     RGAPPARFTDAQWQVLSAQLMTLKLAVAQLRVDFAETGEVDFIEIAQRAQSALGAADD
                     PGELLLKLDASIRHLLVDEFQDTSQTQVRLVQTLTSGWQAGDGRTLFLVGDPMQSIYR
                     FRKAEVGLFLEVAEHGIGEVRLAFLNLTDNFRSQAGVVEWVNATFQGLFPQVSEAAAG
                     AIVYTRSQPFNPPLAGPAVQFHVAFGETRAADADQQAEETALALVRQALAQADPSSRH
                     PVAILVRARGHLGRLVRRLSREGIACRAVDLVPLARREVVADLVQLMRALAHPADRLA
                     WLSVLRAPWCGLTLASLHALFGHDLRTPVPALLAEALRVPRGEQQAPQRGLWDAASEL
                     CPAERVLPPDELLRLRRVAGILLDTSNGSGALPWAAWIEALWLRLGGAALYADAGAAD
                     DAESLFALVERLAPHGGLDAARLDAALSRLYAAPEAGEEQGRAVEIMTMHKAKGLQFD
                     TVILYGLHRTPARSQPPLVRIEQSGERVLFGPVKPRADKDADPISRYLAQREQRRADY
                     EVDRLLYVAATRARHHLHLVANAVVDPKTGAAKAPASSSLLARLWDVVPPEKKQPPVQ
                     ADAQDMAAEPQTASRGEPLRRLAADAVAHLAAQSWRQGNPGFGVPVPGSRSWGQGADR
                     PAWQLEEAYDAAIGTLAHAWLARIGMDGAGQWTPDALRARLPQMQRQLTRAGVPQALA
                     GESAEAVLETLLATLADERGRWLLSQSGARREWPLIDASGKVSVIDLAVSTAGGWLIV
                     DYKTGRPHPGESLADFADRMRHRHGDQLERYCRHVTAWDGRPAQAALYFPRAKLWVEF
                     "
     gene            complement(11401..14127)
                     /locus_tag="VGC69_07380"
     CDS             complement(11401..14127)
                     /locus_tag="VGC69_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0295140.1"
                     /translation="MSDDNETLVRRMAALPEIAALPAVDTLVLTVNNRLARRLVQELA
                     AYLRSERQVSELPRVLPLSVWLGAAADDLAFVPDAGDVDILPAYRLDAFAAQLLWARA
                     IAEEEAGRVLLDTTQAARLAADADALMAEWRLRIPAGADTDEYRSYARWRVRYRNALR
                     EIDAEDANRIYERVLEAVRQRTLPMPRFVVLAGFSDESPRFAELLRAIRDQGASLVWL
                     AAEQRDAAVPGRYVARDHAAEWRAAAAWAAQRLTENPAGRYAIVSAQLEDESPYARRV
                     LGRALAAGGHAYNVAVGRPLSEWPAARAALAWLDALAHMAEAGGADAPVLGAALLAGH
                     CAGDGREAARHAAIDARWRRNGVLRLTPAKWLRDLDGSPLAEAWTQAVQDWQAFAGRG
                     PQASDAWVPVMRAALGALGFPGERALDSVAYQVVGALGDLLGRYGALAPAAGLLDGRA
                     AVRLLARAAQASSFQPQRDPSARLDVLGLLEAEGGAWDGMWLLGLTDDVLPASPKPNP
                     LLPLPVLRAAGAPRATPERERKWAENLVAALCTCAPEIVVSHAQHEGESELRPSPLIA
                     DLPCTSWWMPGTAGAAAWPQQMLDDDRGPPLPEVDAAAGRGAAGGLDVLDTQARNPLW
                     AFVRHRLGGRAMADYAQGADASQRGQFLHRALELVWRMLPDQEQLHAAMAEGRFGPLL
                     DESIAQSAEEILVGYGAALRELECQRARVVLAAWFDIEARRTPFKVAQVEEQHQWTHG
                     ALRLSVRLDRLDRLPDGRAVIVDYKTGMSPGKPESDWSRARPVNLQLPFYASVLAGEE
                     PEAGVAALVLAQVHARQVAAQGLSDDDIELGLDGVKAARDSQDFSGREWRAILAGWRA
                     AIEGLAGEYAEGYAANFTVNPQDLQYCDAMPFLRLNLESAELGGEDEDGEGT"
     gene            14241..15194
                     /locus_tag="VGC69_07385"
     CDS             14241..15194
                     /locus_tag="VGC69_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEY0295141.1"
                     /translation="MAGALAACLLLAGCSSLPPSDAGQPAAASESRWLDATPRIAVVS
                     AFAPELALLTSRLDHARTYRVNGVQFSAGLLQGKPVLLFLSGVSMTNAAMNTQLALDR
                     FRVSHLVFSGIAGGVNPALHIGDVAVPAQWGPYLEATMARETAPGKFTPPAWQKDFTA
                     PPFGMLYPRPVEVRSAAQPDLHKKFWFEVDPVLLDRARALSDAVRLNRCAATGTGGQT
                     CLAHEPRLVVGGNGVSGSAFVDNKALREYAFRRFKTDVLDMETAAVGQVAYANGVPFI
                     AFRSLSDLAGGGEGENEMDTFMKIAADNAAEVLLAFLGAWR"
     gene            complement(15207..15605)
                     /locus_tag="VGC69_07390"
     CDS             complement(15207..15605)
                     /locus_tag="VGC69_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295142.1"
                     /translation="MAVCADAFYGLLGALGVAQDSRARAFGTTFFLTLSNPMTIFSFI
                     GIFAALGSSLGTGQGGGPAGWVSVAPMVAGVLAGSAAWWLLLSGASAMLRKKMPARLA
                     SGVALLSALAIAAFGVTQFVSGLMHVLRSA"
     gene            15727..15924
                     /locus_tag="VGC69_07395"
     CDS             15727..15924
                     /locus_tag="VGC69_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295143.1"
                     /translation="MQSMNLHTTCHACGWQKSIPQHGDTPIEPAACLQCGSTDIENIW
                     APGDPLQNALSDAINRVFGKS"
     gene            complement(15962..16948)
                     /locus_tag="VGC69_07400"
     CDS             complement(15962..16948)
                     /locus_tag="VGC69_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0295144.1"
                     /translation="MTTPLLEVSGLKKYFNIGGGLFGGTRRVHAVDGVSFSLSRGETL
                     SIVGESGCGKSTVARAVLGLSDVTDGQVRLDGQRIDGLSRRALRSVHRRLQVVFQDPF
                     SSLNPRMRVRDILTEPLRNFGLASDASDIDARLKRLMDVVQLTREGLDRYPHEFSGGQ
                     RQRIGIARALASEPDVIICDEAVSALDVSVKAQIVNLLQDLQKQLGLALLFISHDLAI
                     VEHMTHRVAVMYLGRIVEMGTRRQIFLSPRHPYTQALLSAVPVPQPGAARRRIMLKGD
                     VPSPIAPPPGCHFQTRCPYVTERCRTEPPVLRRSEEGHWWACHLDELPAVSG"
     gene            complement(16945..17937)
                     /locus_tag="VGC69_07405"
     CDS             complement(16945..17937)
                     /locus_tag="VGC69_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295145.1"
                     /translation="MPILEVENLQTHFRTPDGVNRAVDGVSFSVEAGETLAIVGESGC
                     GKSVTSMSILRLIPEGVGKSAGAIRFQGRDLLSLSEDEMRDIRGNSISMIFQEPMTSL
                     NPVLTIGHQVDESLRLHEGLNKEQARARTVGLLRMVGIPEPERRAREYPHQLSGGMRQ
                     RVMIAMALACSPKLLIADEPTTALDVTIQAQILDLMRGLKQRVGAAIVLITHDLGVVA
                     EVADRVVVMYAGRKVEEAAVGELFDHPLHPYTQGLLGAVPRLGASREEEMGRLSEIPG
                     LVPSLKRRIQGCVFAGRCPRVQPVCRTVAPGLRDARERHPVACHFAGQVLESVA"
     gene            complement(17937..18839)
                     /locus_tag="VGC69_07410"
     CDS             complement(17937..18839)
                     /locus_tag="VGC69_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295146.1"
                     /translation="MGAVPVNLVIAPDLPDVLPEPRVRKGFSGFCRRHPQMLFALALL
                     SLLVLMAVFAPLLAHADPNALSPARRARPPSAQYWFGTDMLGRDIYSRIVYGARVSLI
                     VGFCVALFASAAGLAVGLVAGFLRWADGIVMRVMDGMMAIPPILLAIALLAVSHGSMG
                     NVIVALTISEIPRISRLVRGVVLSLRDQPFIDGAIASGNRTMRIITRHVLPNTLSAIT
                     VQATYICASAMVLEAILSFIGAGVPTTTPSWGNIMADGRALWQVKPYIIIFPAVFLSL
                     TVLAVNLLGDGLRDVLDPRMEKAL"
     gene            complement(18857..19798)
                     /locus_tag="VGC69_07415"
     CDS             complement(18857..19798)
                     /locus_tag="VGC69_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295147.1"
                     /translation="MLKYIVKRILATIPVLCVVGVFIFSLLFVAPGDPAAILAGDQAT
                     QQDLQRIRQEMGLDRPFAVQFMDWAGHMLRGDMGHSVFNKQPVAHLIGSSLEPTLLLM
                     VMTIVLAVAVGVPMGVVAAFRHGGRLDTLVMSMSVLGFSVPVFVIGYILAYVFALRLG
                     WLPVQGYVPLSESVWQAVRSLILPAFTLSGMYVALIARTSRAAMLEVLQQDYIRTARA
                     KGVSMRDILFVHALRNCGVSILTVVGVGIALLIGGAVVTESVFAIPGLGRLVVDAILQ
                     RDYPVIQGVVVFFSLLYVMVNLIIDLLYTLVDPRIRY"
     gene            complement(19829..21358)
                     /locus_tag="VGC69_07420"
     CDS             complement(19829..21358)
                     /locus_tag="VGC69_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295148.1"
                     /translation="MAPRFAFAQEGGKSVLKFVPQANLTILDPVVTTAAVTVTHGYCV
                     FDTLYGVNGKLEPKPQMAESAQVSADGLTWTIKLRDGLAFHDGQPVRSRDCAASLKRW
                     GQRDTSGQELAKAVADYDTSDDRSLVIKLKKPFPRMLFVLSKPHSSPAFMMPERLAGL
                     SASEPLTEMVGSGPFRFVAAEYVPGSKAVYEKFEGYKPRSEPADWTSGGKVVHFDRLE
                     WHIIPDSATAASALQNGEVDWWEVPLPDLLPVLRSNANIRTRVSDPYGMIVVMRFNSA
                     QAPFNNPKVRRAVYEAVNQSDYMNSLGLDEQDYKECLSNYPCGMPGVKNALLGKPGGL
                     DKARQLLKESGYAGEKVVIINPTDNIMIGPLGHVTADLLKQMGFNVDLQDMDWGSVTQ
                     RRTSRESVDKGGWSIFHTTWPSVSVGDPSLNSNVRGQGKDGWFGWFDDPEIEKLASQW
                     MDAQDPAEQDHLFEESQRRALEMMPVVMLGEFTIQTALRKNLTGDLPGSACFFWGIRS
                     A"
     gene            complement(21517..23010)
                     /locus_tag="VGC69_07425"
     CDS             complement(21517..23010)
                     /locus_tag="VGC69_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEY0295149.1"
                     /translation="MVSGRKRVAIASFEFEGNDFSPKINDLALFNLYAVGGDVFARIE
                     GRPLAVTGAVEVLREEGGLDLVPILVAHGGSGGKVEDDFYRRTTDAIVAGIREQGPFD
                     GVYLALHGAMVSGGVKDAEGELLSAVREIVGSGVPIAASLDLHAHVSARMVRTADILV
                     GYANYPHDDAYDTGKRAAALLRDTLAGKLRPVMRMTRLPMLVPVTGSCTRYDEAPLAR
                     VKKLARSLETDGVVSTSYFTVQPWLDDDEAGNVALAITDGDAGRAEGVSRQIAQHMWD
                     LREEFELPLLSVESAIEQARGATVRPFIFADTADCVGAGASGDAAYVLAALLKHAGSL
                     RCAVSLVDAQVAAEAHAAGAGAVLDATLGFRADPRYGEPVPVKATVVNLHDGSFTYVG
                     GPSGGVVGDMGPTAVLQAGGVRVLVASKPTYEYADEQFRAAGIDPDDCDVLVLKNGMN
                     FRNLLGPRTPWVLLDSVGASPAGLGALPWRNRPSPFWPRDRNFADPF"
     gene            23586..>24702
                     /gene="hisD"
                     /locus_tag="VGC69_07430"
     CDS             23586..>24702
                     /gene="hisD"
                     /locus_tag="VGC69_07430"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012337431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0295150.1"
                     /translation="MIRKLKAAKPAQVQDAIAREVQQVVAGIIADVTQRGDVAVREYS
                     VKFDRWNPEAFCLNDAQIADCIAQLSARELDDIKFAQAQVRRFAEAQKAALTDIEIET
                     IPGVVLGHRNIPVDSVACYVPGGKYPLIASAHMGVVTAKVAGVGRIVAVAPPFEGKPN
                     PAIVAAMHLGGADEIYCIGGVQAVAALALGTDTFRPVDMIVGPGNAYVAEAKRQLFGK
                     IGIDLIAGPTETLLLTDHTCDAEMAAADLLGQAEHGVNSPAVLVTTSKQLAEALPAEI
                     ERQLAVLPTAAIAGVAWQDYGEIILCDTLEEMVQEGNRIASEHVQVLTEDPEYFLEHL
                     TNYGALFLGARTTVSYGDKVIGTNHTLPTMGEARYTGG"
BASE COUNT         4240 a         8738 c         8024 g         3700 t
ORIGIN      
        1 gccagcgcgc tggcggcgcc ggcgagccgg gtgatttccg tccagtcccg cgccgccacg
       61 gccgccttgg gcgtcagcca cgagccgccg acgcacacca cattgggcag ggccaggaaa
      121 gtcggcgcgt tttcggcgtt gacgccgccg gtggggcaga acttcagcga agggaacggg
      181 ccgtgcaggg cattgagcat gggcacgccg ccggcctgct gcgccgggaa gaatttgacg
      241 atgtcgtagc ccagctcgag cgcgagcagg atgtcgctgg gcgtcatgac gcccggcaag
      301 agcggcaggc cggcgtcctg cgcggcctgg tgcaggtcgc gcgtgaggcc cggcgacacg
      361 ccgaaacgcg cgcccgcttt cctggcctgc tcgcactggc gcggcgaggt cagggtgccc
      421 acgcccacca cgatgtcggg cgcgaggccc gccgcccgtt cgatggcggc caggccggcc
      481 tcggtgcgca aggtgatctc cagcaccttg acgccgcccg cgtgcagggc gcgcgagacg
      541 ttttcgcctt gctcggcgga ttcgaacgcc aggaccggga tgaccggtcc gatgccgacc
      601 aggccggcga ccgtcgttgc cgccggtgtt gccatcgcag ttgccatgaa tgagactcct
      661 agaaaccaaa gaccgaggcg cccgactccg cgggcgcggc ggcggcgcgg aacacgccga
      721 acagttcacg gccgaagccg acttcgttgt ctgcctgatg cgcgggctgg gccgagggcc
      781 gcgcggccca ttgcgccgcg tcgatctcga cgtcgagcac gcccgcttcc gtgtcgagca
      841 gcagcatgtc gccgtctcgc accttgccga tggggccggc gagcagggcc tcgggcgaga
      901 ggtggatgac cgccggcacc ttgcccgacg cgccggacat gcggccgtcg gtgaccaagg
      961 ccaccttgaa gcccttgtcc tgcagcacgc ccaggaaagg cgtgagcttg tgcagctcgg
     1021 gcatgccgtt ggcgcgggcg ccctggaaac gcaccacggc gacgaaatcg cgctccagtt
     1081 cgccgcgctc gaaggcggcc ttcaccgcat cctgcgagtc gaacaccacg gccggcgcgc
     1141 gcaccatgcg gtgctcgggc ttgaccgccg agaccttgat cacgccgcgg cccagcttgc
     1201 cctgcatcag gcgcaggccg ccgtcgggct ggaaaggatc ggagatcggg cgcagcacgt
     1261 cgctgtcgcc gctttgcgcg ggcgccgggc gccagcgcag ctcgccgtcg accagctcgg
     1321 gattgtgcgt gtagcgcgac aggcccttgc ccatgaccgt ggcgacgtcg tcgtgcagca
     1381 ggccggcctc gagcaggttg cgcaccagga aggcgacgcc gcccgccgcg tggaaatggt
     1441 tcacgtcggc cttgccgttg gggtagacgc gggccagcag cggcacgatg cgcgagagcg
     1501 tatcgaagtc gtcccagtcg atcacgacgc cggcggcccg cgccatggcg accaggtgca
     1561 gcgtgtgatt ggtggaaccg cccgtggcca gcagcgcgac gatgccgttg atgatggcct
     1621 tctcgtcgat gacgtggccg atcggcgtgt attgcccgcg ttcggccgtg atctcgagca
     1681 cgcgccgggc ggcggcggcg gtgagcgcgt cgcgcagcgg cgtgtgcggc ggcacgaagg
     1741 ccgagcccgg caggtgcatg cccatgactt ccatcagcat ctggttgctg ttggcggtgc
     1801 cgtagaaggt acaggtgcca tggccgtgat aggccgccga ttcggcctcc agcagcgcgt
     1861 cgcggccgac ctggccggtg gcgtactgct ggcgcaccct ggccttttcg tcgttggaca
     1921 ggccgctggt catgggaccg gacggcacga agatgacggg caggtggccg aactgcagcg
     1981 cgcctatcat caggccgggc acgatcttgt cgcagatgcc caggcacagc gccgcgtcga
     2041 acatattgtg ggtgagcgcc accgccgtgc ccatggcgat gacctcgcgc gagaacagcg
     2101 acagctccat gccggcatag ccctgcgtga cgccgtcgca catggcgggc acgccgccgg
     2161 cgaactgcgc cacgccgccg ttttcacgcg cggcgcgctt gatgatctcg ggaaagtcgc
     2221 ggtacggcgc atgcgccgag agcatttcgt tataggccga cacgatgccg atattgggct
     2281 cgcgcatggc cttgatcatg atcttgtcgc ggtcctcgag gccggcgaaa ccatgcgcca
     2341 ggttcgagca cgacagcgcg ccgcgcacgg gaaagcggcc gcgggccgcg tcgatgcggg
     2401 ccaggtaggc gtcgcgcgtg gccttgctgc gctcgatcac gcggcgcgtg acggcctgca
     2461 gcgaggtaag gggggaaggc atgggatttc cttttttgcg cgggcggcgg cttgcgtgcc
     2521 gcccggggcc cgtcgcaagc accaaaagta gaaaaactac acgccttttg gggcggattc
     2581 cagatcggcc atcgcgaatt tcgcgagatt atatcggcta aacgaaacaa gtttccggta
     2641 ggaaacccgg ccccgccagg aaccggcatt tgtagttttt gtacaaatcc ggaaagacct
     2701 gcttatagtt gtgggcaaat atcctcagga tttccatgct gctttcccag gtccatgcca
     2761 tgcgcgacca gttgcgccca tccgagcgca agctggccga ctacatcatc gaggcgcccc
     2821 gcgaggtgct cgacctttcc atgtccgagg ccgcgcagcg cgccggggtg agccagccga
     2881 cgatcgcccg cttctgccgc gcgctgggct tctcgggctt tcgcgagttc aagatccgcc
     2941 tggcccaggc cgtggcctcg caactgcccg ccgtgcatcg cgacgtgcgg gccgacgagc
     3001 ccgtgtccgg cctggccgcc aaggtgctgg atcgcaatat cgcggcgctc atccacgtgc
     3061 gcaacacgat gtcggtcgac gcgctggcca tggccatcga cctgctggcc caggcctcgc
     3121 gcatcgagtt ctacggcgcg ggcggctcgg gcatctccgc gcaggacatc cagcacaagt
     3181 tcttccggct gggcatgtcc agcgtagcgt attccgaccc gcatatcttc ctgatgtcgg
     3241 ccgccctgct cggcccgggc gacgtggtgg tggcggtgtc gacctccggc cgcaccgtgg
     3301 acatcgtcga cgccgcccgc gcggccctgt ccggcggcgc ccaggtcatc gccatcaccc
     3361 gcagccactc gccgctcgcc cgcctggcca ccatcagcct gcacgccaac gtcgaggaaa
     3421 ccaccgacgt cctctcgccc atgacctcgc gcgtctccca cctggccatc ggcgacatcc
     3481 tcgccgtcgg cgtcgccctg cagcgcggcc cccagctcct ggaaaagata tcccactcca
     3541 aaaccctgct caagaacaga agaatctccg aacaaagctg agaacccgtt ccccaacatt
     3601 aagaactccg tcccccgaca gcaaccactc gacctccctc ccgtgaggga gggctggggc
     3661 gggcgggcca gctacaggtc ccaaggggac ccaccacccc gaattcaaac agacgggctc
     3721 cgcccgctct gttagaattc ggggtggtgg gtcccttcgc atggtgcggc ggtgaacctg
     3781 gtcaggtcgg gaacgaagca gccatagtcg tttagcgcca gtgccgaagt aaggctcacc
     3841 accccatacc ctaaagggcg ccgatggcgc cctttttcat ggctgcgccc aggccagcgc
     3901 aactggctac aatccaacca tgacttactt ggtactggcc cgcaaatggc gcccgcgatc
     3961 cttcgacacc ctggtggggc aggatcacgt ggtgcgcgcc ttgacgcacg cgctgcaaac
     4021 ccagcgcctg caccacgcct ggctgttcac cggcacgcgc ggggtgggca agaccacgct
     4081 gtcgcgcatc ctggccaagt cgctcaattg cgagaccggc atcacgccca cgccctgcgg
     4141 cgtgtgccgc gcgtgcaccg agatcgatgc cgggcgtttc gtcgactatc tggaactcga
     4201 cgcggcctcg aaccgcggcg tcgaggaaat gacccagttg ctggagcagg cggtctactc
     4261 gccgggcgcg gggcgcttca aggtctacat gatcgacgaa gtccacatgc tcaccgggca
     4321 tgcgttcaac gccatgctca agacgctgga agagccgccg ccgcacgtca agttcatcct
     4381 ggccacgacc gacccgcaga agatccccgt cacggtgctg tcgcgctgcc tgcagttcaa
     4441 cctcaagcag atgccgcccg acgccatcgt gggccatctg cagaatgtgc tgggccagga
     4501 aaacgtcccc ttcgaagtgc ccgcgctgcg gctgatcggc caggccgcct ccggctcgat
     4561 gcgcgacgcc ctgtcgctca ccgaccaggc catcgcctat agcgccggcg acctcaccga
     4621 ggaatcggtg cgcggcatgc tcggcaccat cgaccagcgc cacctggtgc ggctgctcga
     4681 ctccctggcc gacggcgacg ccaccggcgt gctggccgtg gccgacgaac tgtccacgcg
     4741 cggcttgtcg tatgccggcg cgctggccga cctggccgtg ctgctgtcga agatcgccat
     4801 cgcccagcgc gtgccgggcg ccttgtccgc cgaggacccg atcaccgccg acaccctgcg
     4861 gctggcgggc cgcctgcatg ccgacaccgc gcagctgttc tattccgtgg ccgtgcacag
     4921 ccgcggcgaa ctgacgctcg cgcccgacga atacgccggc ttcatcatga cctgcctgcg
     4981 catgctggcg ctcaatggag acgccggccc ggccacaatc gtgcaggcgc ccgcggtaat
     5041 gcccgccgcc agcgcaccgg ccgccacctc ggcggcggcg cccgcgcccg ttgccgcgcc
     5101 ggaaccgaag ccggttgcgg cggcgccctt gctggcccct gcccggcccg aaaccccgcg
     5161 cgccacggct gccgtcgaac cgcccaaggc gcccgccgcg gcgccggccc cgttaccggc
     5221 cccgacgcca gccaagcagg ccgacacgcc gccctgggat gacacgccgg cggccaaggc
     5281 catgcctgcc gcgccggtgg cgcaagccgt cccggacgag cctaccgccg ccgacagccc
     5341 gccggcctgg gtcgatgaag acatccccga cgatgcgggc ggctacgacg caggcgccgc
     5401 cttcacggcc gaccccgacg acgaattcga aaccctggcc ggcgcggccg cgcccagggt
     5461 cgacacgccc cgccccgtca ggcccgcgcg ggccgcgcgc gaaccggcgc aggcgcgcgc
     5521 gcgcctgacc gacatgacgc aggccgcctg gcccgagctg gcggcccgcc tgcctgtaag
     5581 cggcctggcc gccgagctgg cccgccagag cgcctggggc ggcctgcagg gcgacaccgt
     5641 catcctgcgc gtggccgtca agacgctggc cgagagcgag agccgcgtgc gcttgcagac
     5701 ggtactgtgc gagcattacg gccaaagcct gcggctcgag gtcgccgtcg gcgaaaccgg
     5761 cgaggccacc gcccacgccg tggcgcagca ggaacgcgcc gcgcgccagc aggccgccga
     5821 agacgccgtg gccgccgatc ctttcgtgca ggccctggtc agcagcttcg gcgcccgcgt
     5881 ggtgcccggc tcgatacggc ccgtcgaccc gcccgcggcc tgaacacaac accgggggcc
     5941 gctcaccgcc gcccttcccc ttagccgata ctcaaggaag cttccgacat gatgaaagga
     6001 caactcgccg gcctgatgcg ccaggcgcag cagatgcagg aaaacatgaa gaaggcccag
     6061 gaagccctgg ccgacgtcat ggtcgagggc gccgcgggcg gcggcctggt caaggtgacc
     6121 atgaactgcc gcaacgacgt caagcgcgtg gccatcgacc ccagcctgct gggcgacgac
     6181 aaggacatgc tcgaggacct ggtcgccgcg gccttcaacg acgcgctgcg caaggccgag
     6241 gccacctcgc aggaaaaaat ggccggactg accgccggcc tgccgctgcc cccgggcatg
     6301 aagctcccgt tctgatctct cgccatggag tcgcgccctc cgcgcggctc cgccatgcat
     6361 catggatccc cagctccccg agcccgaacc cctgcgtgcc ctgatcgagg ccctgcgccg
     6421 cctgcccggc gtgggcgtgc gctcggcccg gcgcatggcc taccacctgc tgcagcacga
     6481 cgtgcagggc gccgacatgc tgggacgcgc gctttccggc gcggtgaaca acctgcgcca
     6541 ttgcgcgcgc tgcaacagct tcaccgaaga cgagttgtgc gccacctgcg ccaacccccg
     6601 gcgcgacccc ggcctgctct gcgtggtgga gacgccggcc gaccagaaca tgatcgagtc
     6661 cagccacggc taccgcggcc tgtactacgt gctgatgggc cgcatcgcgc cgctggaagg
     6721 cataggcccg cgcgaactgg acttccagcg cctgctggac cgcgcctgcg acggcgtggt
     6781 gcaggaagtg atcctggcca ccaacttcac ggccgaaggc gaaacgacgg cgcatttcat
     6841 cggcgaggcg ttgagctcgc gcggcttgaa agtcacgcgg ctggcgcgcg gcgtgccggc
     6901 gggcagcgaa ctggaatacg tcgatgccgg gacgatcgcc tgggcgttga tggagcggcg
     6961 gtcgacctga tcaggccgtg ttccgggcca gccacaaggc aacgcccagg acgttctgcg
     7021 agtcggattc cgtgccgatg gtattggggt aggtgggcag cgcgttggga ttgtagtttt
     7081 ccaccaccca ggccttgggc aaggcatcgt gcttggacag ataggcgacg gcctgcctgg
     7141 tctcttccag gaatcggatg ccggacgcat tgggcgaaat gatgaacgtg gtgtgcaggc
     7201 ccgcagcgtt gccccacttc aggttttcaa cgacaaactg gcgataggct tcgggctgcg
     7261 ccaggaaata gtgcggcggc gagtccgtcg tcaccgcgcc gcccgctttt tccccttcgc
     7321 gcaggtaatt ccacttgggg cttgaaaacg gcgcgctcgc atattggcgg ctattgggcg
     7381 aaaagatcgg cgccaccgtc tcgatgcccc ggcggcgcgc ggcggacacg aaatgctgcc
     7441 agtcgtccag gcgattctcg gcaaagccat tgacgtgcga gtaccgaacc tggatgccga
     7501 catccgtgaa cagtttcttg tacggctgcg cgaaccaggt ttccggattg ttcatcaggt
     7561 tgaactcggc atcgaccggc gcgccggcga aggtggccag gatagccttg tgcaagtctt
     7621 ccttcgttcg cgcccagatg tactcgtgga tgtagagcgc ggtgcggcag ctctgccgca
     7681 gcgtccggct gtgcgcaggg tcagccaggg aatcgagtcc ggtcacgccg cccaccgata
     7741 tgggcgtgcc ttgccccgaa cacgaatacg cctgagcctt gcgcacccag ggcgccaggg
     7801 tcgctgccgc gccaaatgcc aggaattgcc gccgggaccc tgtcatcatg tgccgccctc
     7861 tcacgagatc acaggtccca tgtggcagcc gatcaaaact cgacccacaa cttcgcccgc
     7921 gggaaataca gcgccgcctg ggcaggccgg ccgtcccagg ccgtgacgtg gcggcaatag
     7981 cgttccagct ggtcgccatg gcgatggcgc atgcggtcgg cgaagtccgc cagcgactcg
     8041 ccgggatgcg ggcggcccgt cttgtaatcg acgatcagcc agccgcccgc cgtgctcacg
     8101 gccaggtcga tcaccgacac cttgccgctg gcgtcgatca atggccattc gcggcgcgcg
     8161 ccggattgcg acaatagcca gcggccgcgc tcgtcggcca gtgtggccag cagcgtttcg
     8221 agcacggcct cggcggattc gccggccagc gcttgcggca cgccggcgcg cgtcaactgg
     8281 cgttgcatct gcggcaggcg ggcacgcaag gcatcgggcg tccactggcc ggcgccgtcc
     8341 atgccgatgc gcgcaagcca ggcatgggcc agcgtgccga tggccgcatc gtaggcctct
     8401 tcgagctgcc aggcgggccg gtcggcgccc tgcccccacg agcggctgcc cggcacgggc
     8461 acgccgaaac cgggattgcc ctgccgccag gattgcgccg ccaggtgggc caccgcatcg
     8521 gcagccaggc gccgcagcgg ttcgccgcgc gatgccgtct gcggctcggc cgccatgtct
     8581 tgcgcatcgg cctgcaccgg cggctgcttt ttctctggcg ggacgacatc ccacaaacgc
     8641 gccaacaggc tgctcgaggc cggcgccttg gccgcgccgg tcttggggtc gacgaccgca
     8701 ttggccacca ggtgcaagtg atgccgcgca cgcgtggcgg ccacatagag caagcggtcg
     8761 acctcgtagt cggcgcggcg ctgctcgcgc tgggccagat agcgcgagat ggggtcggca
     8821 tccttgtccg cgcgcggctt gaccgggccg aacagcacgc gctcgccgct ttgctcgatg
     8881 cgcaccaggg gcggctggct cctggcgggc gtgcggtgca ggccgtagag gatcaccgta
     8941 tcgaactgca ggcccttggc cttgtgcatg gtcatgatct cgacggcccg gccctgctct
     9001 tctccggctt cgggcgcggc atacaggcgg gacagcgccg catccagccg cgccgcgtcc
     9061 agcccgccgt gcggggccag gcgttcgacc agcgcgaaca ggctttcggc atcgtcggcc
     9121 gcgccggcat ccgcgtacag ggccgcgccg cccaatctca gccacagggc ctcgatccag
     9181 gcggcccacg gcaaggcgcc gctgccgttg gacgtatcga gcaggatgcc ggccacgcgc
     9241 cgcaggcgca gcagctcgtc gggcggcaac acgcgttcgg ccgggcaaag ttcggaagcc
     9301 gcatcccaca ggccgcgctg cggcgcttgc tgctcgccgc gcggcacgcg caaggcctcg
     9361 gccagcaggg ccggcaccgg cgtgcgcagg tcgtggccga acagcgcgtg caacgaggcc
     9421 agcgtgagcc cgcaccaagg cgcgcgcaac accgacagcc aggccaggcg atccgcggga
     9481 tgcgccaggg cgcgcatcag ctgcaccagg tcggccacca cttcgcggcg cgccagcggc
     9541 accaggtcca cggcgcgaca ggcgatgccc tcgcgcgaca ggcgccgcac cagccggccc
     9601 aggtggccgc gcgcgcgcac caggatggcc acgggatggc ggctggatgg atcggcctgc
     9661 gccagggcct ggcgcaccag ggccagcgcc gtctcctcgg cctgctgatc ggcatccgcc
     9721 gccctggtct cgccgaaggc cacatggaac tgcacggccg gcccggccag cggcggattg
     9781 aagggctgcg agcgggtata gacgatggcg ccggccgccg cctcgctgac ctgcgggaag
     9841 aggccctgga aagtcgcgtt cacccactcg accacgcccg cctgcgagcg gaaattgtcg
     9901 gtgaggttga gaaacgccag ccgcacctcg ccgatgccat gttcggccac ctccaggaac
     9961 aggcccacct cggccttgcg gaagcggtag atggactgca tggggtcgcc caccaggaag
    10021 agcgtgcggc cgtcgccggc ctgccatccc gacgtgagcg tctgcaccag gcggacctgc
    10081 gtctggctgg tgtcctggaa ctcgtcgacc agcagatggc ggatggaggc atcgagcttg
    10141 agcagcagtt cgcccgggtc gtcggccgcg cccagggcgg actgcgcgcg ctgggcgatc
    10201 tcgatgaagt cgacttcgcc ggtctcggcg aagtccacgc gcagctgcgc caccgccagc
    10261 ttgagcgtca tgagctgcgc gctcagcacc tgccattgcg cgtcggtaaa ccgcgcgggc
    10321 ggcgcgccgc gcaaggcgtg caacaggccg gtccagcgcg gcgcgtcgag ctcggccccg
    10381 gacagccatt gcgtgaacgc cgtcttgtgg ccgctgttgg gcggaaagcc caggcccttg
    10441 ttgacgccgc gggcgctgcg caggctgccg gcgccggtca gcagcaggtg cgcgagcgcc
    10501 tgccagcgcg gcagttgcgc ggcctcgcaa gccaggtcgt catcccagtc gcgcagggcg
    10561 gccagcgggt tggcgccgcc ggccgcgtcg agttcggcgg cggccaggcg ggcaggctcg
    10621 cgcaaggccg cttgccagcc catcggcagg gcgcgcgccg cggcgcgcag gtcatcggca
    10681 atggcctcgg acagcatgct ttccagctcg tcgcggtcgc cgccgtgggc cagcagcggc
    10741 atccactggt cgcgctgcgc cagcatgccg gcgatggcct cgcccgcggc gcgcacgtcc
    10801 acgtccatgt gctcgagcag tatccgcacg gcctcgaaat cgtcggccag ggccagcgtg
    10861 gcgtgcgccg ccgcggcgta atgcgcctcg gcatcgctgg tgatttcggg catgccgccc
    10921 agctcggaca gccagggcat gctgcgcacg agcgaggcgc agaacgaatc gatggtgcgg
    10981 atggccagcc gcgccggatg atgcagcagc tgccagccca gttccgcgtc gcgcgccagg
    11041 gcggcgcgcg ccagcgccca gctgcgccgg ccgtgctcgc tttccggggc ggggccctgc
    11101 gcctcgcgca gcttgcccag cacgcgcgcg tgcatttcgg aggccgcctt gcgggtaaag
    11161 gtgatggcca cgatttcttc gggccgcgcc accgtggcca gcagggccag gatgcggtcg
    11221 gtcaggagct cggtcttgcc cgagccggcc ggcgcctgca ccaggaacga ggcggcagga
    11281 tcgagcgcgc gcaggcgcgc cgcgagatcg gaaggctgac tctgcgcatt catgacgccc
    11341 ccttggcgct gcgcgccgtc cccccggggg ggaattcgcg cttgaggcgg ctcggcgcgc
    11401 tcatgtcccc tccccgtcct cgtcctcgcc gccgagctcg gccgattcga gattcaggcg
    11461 cagaaagggc atggcgtcgc agtattgcag gtcctggggg ttgaccgtga agttggcggc
    11521 atagccttcg gcgtattcgc ccgccaggcc ttcgatggcc gcgcgccagc ctgccaggat
    11581 ggcgcgccat tcgcgacccg aaaagtcctg gctgtcccgc gccgccttca cgccgtccag
    11641 gcccagctcg atgtcgtcat cggacaggcc ctgcgcggcc acctggcgcg catgcacctg
    11701 cgccagcacc agggcggcca cgccggcctc gggctcctcg cccgccagca ccgatgcata
    11761 gaaaggcagc tgaaggttca ccggccgcgc gcgcgaccag tccgactcgg gcttgccggg
    11821 cgacatgccg gtcttgtagt cgacgatgac cgcgcggccg tccggcagcc ggtccaggcg
    11881 gtcgagccgc acgctcaggc gcagggcgcc gtgcgtccat tgatgctgct cctcgacctg
    11941 ggccactttg aaaggcgtgc gccgcgcctc gatgtcgaac caggccgcca gcaccacgcg
    12001 ggcccgctgg cactccagct cgcgcaaagc ggcgccgtag ccgacgagga tttcttcggc
    12061 cgattgggcg atggactcgt ccagcaaagg gccgaagcgc ccctcggcca tggccgcgtg
    12121 cagttgctcc tggtccggca gcatgcgcca caccagctcg agcgcgcgat gcaggaactg
    12181 cccgcgctgg ctggcgtcgg ccccctgcgc atagtcggcc atggcccggc cgcccaggcg
    12241 atgccgcaca aaggcccata aaggattgcg cgcctgcgtg tccagcacgt ccaggccgcc
    12301 cgcggccccc ctgcctgccg cggcgtcgac ttccggcaag ggcgggccgc gatcgtcgtc
    12361 gagcatttgc tgcggccagg ccgcggcgcc tgccgtcccg ggcatccacc aggatgtgca
    12421 cggcaggtcg gcgatcagcg gcgacggccg cagctcgctc tcgccttcgt gctgggcatg
    12481 gctgaccacg atttccggcg cgcaggtgca cagggccgcg accaagtttt cggcccactt
    12541 gcgctcgcgc tcgggcgtag cgcgcggcgc gcccgcggcg cgcagcacgg gcaagggcag
    12601 caggggattg ggcttgggcg aagcgggcag cacgtcgtcc gtcaggccca gcagccacat
    12661 gccgtcccac gcgccgcctt cggcctcgag caaacccagc acgtccagcc gcgccgacgg
    12721 atcgcgctgc ggctggaacg agcttgcctg cgccgcccgc gccagcagac gcacggcggc
    12781 ccggccgtcg agcagcccgg ccgccggcgc caacgcgccg taacggccca gcagatcgcc
    12841 caaggctccc acgacctggt aggccacgct atcgagcgcg cgttcccccg gaaaacccag
    12901 cgcgcccaac gccgcccgca tgaccggcac ccaggcatcg ctggcttgcg gaccgcgccc
    12961 ggcaaaggct tgccagtcct gcacggcctg cgtccaggcc tcggccagcg gactgccgtc
    13021 caggtcgcgc agccatttgg ccggcgtcag gcgcaagaca ccgttgcgtc gccagcgggc
    13081 atcgatcgcg gcatggcggg ccgcctcgcg cccatcgccc gcgcaatgcc ctgccagcag
    13141 ggccgcgccc aataccggtg catcggcgcc gccggcttcg gccatgtgcg ccagcgcatc
    13201 cagccaagcc agcgccgcgc gcgccgccgg ccattcggac aagggccggc ccaccgccac
    13261 gttgtaggcg tggccgccgg cagccaatgc ccggccaagc acgcggcggg catacggcga
    13321 ctcgtcttcg agctgggccg agacgatggc atagcgcccc gcggggtttt ccgtcagtcg
    13381 ctgcgccgcc caggccgccg ccgcgcgcca ctccgccgca tggtcgcgcg ccacgtaacg
    13441 gccgggcacg gccgcgtcgc gctgttccgc ggccagccag acgagcgatg cgccctggtc
    13501 ccggatggcc cgcagcagct cggcaaagcg cggcgactcg tcggaaaagc ccgccagcac
    13561 aacgaaccgg ggcatgggca aggtgcgctg gcgcacggct tcgagcaccc gctcgtagat
    13621 gcggttggcg tcctcggcat cgatttcgcg cagcgcattc cggtagcgga cgcgccagcg
    13681 cgcgtagctg cgatattcgt cggtgtcggc gcccgccgga atcctgagcc gccattcggc
    13741 catcagcgca tcggcatcgg ccgccaggcg cgcggcctgc gtcgtgtcga gcagcacgcg
    13801 gccggcctct tcttcggcga tggcgcgcgc ccacagcaat tgcgccgcga aggcatccag
    13861 gcgataggca ggcaggatat ccacatcgcc cgcgtccggc acgaaggcca ggtcgtccgc
    13921 cgccgccccc agccagaccg acagcggcag cacgcgcggc agttcgctga cctggcgctc
    13981 gctgcgcaaa taggccgcca gctcctgcac caggcggcgc gccaggcggt tgttgacggt
    14041 aagcacgagc gtgtccaccg cgggcaaggc cgcaatctcg ggcaacgccg ccatgcggcg
    14101 gaccagggtc tcattgtcgt cggacatgcc ggcaggataa cctgttcccc tgcccatcga
    14161 aatctgcgaa aaccgtgcgc ccaccccacc cccgaatcga ttgccaggca cgcccgatgc
    14221 cgccgcgccg gcgccgcctc atggctggcg cgctcgcggc ttgcctgctg cttgcgggct
    14281 gttcgagcct gccgccaagc gatgccggcc agcctgccgc ggccagcgag tcgcgctggc
    14341 tggatgccac gccgcgcatc gccgtggtct cggccttcgc gccggagctg gccctgctaa
    14401 cgtcccggct cgaccatgcc cggacctacc gcgtcaacgg cgtgcagttc agcgccggcc
    14461 tgctgcaagg caaaccggta ttgctgtttc tttcgggcgt cagcatgacc aacgccgcca
    14521 tgaacacgca gcttgcgctg gaccgtttcc gcgtaagcca cctcgtgttc agcggcattg
    14581 ccggcggcgt gaaccctgcg ctgcatatcg gcgacgtggc cgtgcccgcg cagtggggcc
    14641 cctatctgga agccaccatg gcgcgcgaaa ccgcgcccgg caaattcacc ccgcccgcct
    14701 ggcagaaaga cttcaccgcc ccgcctttcg gcatgctcta cccgcgcccg gtcgaagtgc
    14761 gcagcgcggc gcagcccgac ttgcacaaga agttctggtt cgaggtcgat cccgtcctgc
    14821 tcgaccgcgc ccgggcgcta agcgatgccg tgcgcttgaa tcgctgcgcg gccaccggca
    14881 ccggcgggca gacgtgcctc gcgcatgagc cgcgcctggt cgtcggcggc aacggcgtgt
    14941 cgggatccgc cttcgtcgac aacaaggcgc tgcgcgaata tgcgttccgc cgcttcaaga
    15001 ccgacgtgct cgacatggaa accgccgccg tcggccaggt ggcctacgcc aatggcgtgc
    15061 ccttcatcgc ctttcgctcg ctgtccgacc tggccggcgg cggtgaaggc gagaacgaga
    15121 tggacacttt catgaagatc gcggccgaca atgccgccga agtgctgctc gccttcctgg
    15181 gcgcatggcg atgagccgcg cggggctcag gccgaccgca ggacgtgcat caggccggag
    15241 acgaactgcg tgacgccgaa cgccgcgatc gccagcgcgg acagcagcgc cacgccgctc
    15301 gccagcctgg ccggcatttt cttgcgcagc atggcggacg cgccgctgag cagcagccac
    15361 cacgccgccg aaccggcgag cacgccggcg accatgggcg cgaccgaaac ccatcccgcc
    15421 ggcccgccgc cctggcccgt gcccaaggac gacccgagcg ccgcgaagat cccgatgaac
    15481 gaaaagatcg tcatgggatt ggaaagcgtc agaaaaaaag tcgtgccgaa tgcgcgcgcc
    15541 cgcgagtcct gcgccacgcc gagcgcgccc agcagcccat agaacgcatc cgcgcaaacc
    15601 gccatgataa gtggcgcacc gccatcaagg aattggatag ttgaggtaaa acatgatccg
    15661 gctggcggca ccggacatcg ccgaatccgc agcgagaaag ccgcatcaga gtagaagaac
    15721 ccccctatgc aaagcatgaa ccttcacacc acctgccacg cctgcggctg gcaaaaatcc
    15781 atcccgcagc acggcgatac accgatcgaa ccggcagcct gcctccaatg cggcagcacc
    15841 gacatagaaa acatatgggc gcccggcgac ccgctgcaaa acgcgctgag cgatgccatc
    15901 aaccgggtgt tcgggaagag ctagccgata tcgctttcaa gattggaaga taaaaattca
    15961 ttcagccaga taccgcgggc aattcatcca gatgacaagc ccaccaatgg ccttcctcag
    16021 accggcgcaa caccggcggc tccgtgcggc agcgctcggt gacatagggg caacgcgtct
    16081 ggaaatgaca acccggtggc ggcgcgatgg ggcttggcac gtcgcccttc agcatgatgc
    16141 gccggcgcgc cgcgccgggc tgcggcacgg gcacagccga cagcaatgcc tgcgtgtagg
    16201 ggtggcgcgg acttagaaag atctggcggc gcgtgcccat ttcgacgatg cgccccaggt
    16261 acatcacggc cacgcgatgc gtcatatgct ccacgatcgc caaatcatgg ctgatgaaca
    16321 acaatgccag gcccagttgt ttttgcagat cctgcagcag gttgacgatc tgcgccttca
    16381 ccgacacgtc cagcgccgaa actgcctcgt cacagataat gacatcaggc tcggatgcca
    16441 gcgcgcgggc gatgccaatg cgctggcgct ggccgcccga aaactcgtgc ggataacggt
    16501 ccagcccttc gcgagtcaat tgcacgacgt ccatcagccg cttcaggcgg gcgtcgatgt
    16561 cggaagcatc cgaagccagg ccgaagttgc gcaatggctc ggtcagaatg tcccggaccc
    16621 gcatgcgtgg attcaagctg gagaaagggt cctggaaaac cacttgcagc cgccggtgga
    16681 cggatcgcag cgcgcgacga gacaggccgt cgatacgctg gccatcgaga cgaacctgac
    16741 cgtccgttac gtccgacagt cccagcacgg cgcgtgccac ggtggacttg ccgcagccgg
    16801 attcgcccac gatcgacaac gtctcgccgc gcgacagcga aaacgacaca ccgtcgaccg
    16861 catgtacgcg gcgagtgccg ccaaacaggc cgccaccgat attgaaatat tttttcagcc
    16921 cggatacttc aagcaagggc gtcgtcatgc cacactctcc aagacttgtc cggcgaaatg
    16981 acaggcaacc ggatgccgtt cccgcgcgtc gcgcagaccc ggtgccacgg tgcgacacac
    17041 aggctgcact cgcgggcagc gtccggcgaa cacacagccc tggatgcggc gcttcaggct
    17101 cggcaccaaa ccaggaatct ccgacagccg ccccatctct tcctcgcgcg aggcgccaag
    17161 ccggggcacg gcgccgagca gtccttgcgt gtatggatgc agcgggtgat cgaacagctc
    17221 tccgacagcg gcctcctcga ccttgcggcc ggcatacatc accacgaccc gatcagccac
    17281 ttcggcgacc acgcccagat cgtgagtgat gagcacgatc gcagcgccaa cacgctgctt
    17341 gaggccgcgc atcaagtcga ggatctgcgc ctggatggtg acatccaagg cggtagtcgg
    17401 ctcgtcggcg atcagcagct tggggctgca cgccagcgcc atggcgatca tgacgcgctg
    17461 ccgcatcccg cccgatagtt gatgcggata ctccctggcc cggcgctcgg gctcgggaat
    17521 gcctaccatg cgcagcagtc cgacggtacg ggcgcgggcc tgttctttat tcagtccttc
    17581 atgcaaccgc aggctttcgt cgacctgatg gccgatagtc agcactggat taaggctggt
    17641 catgggctcc tggaagatca tggagatgct gttgccgcgg atatcgcgca tctcgtcttc
    17701 ggacaaggac agcagatccc tgccctgaaa ccggatcgcc cccgcgctct tgccgacacc
    17761 ttccgggatc aaacgcagga tggacatcga cgtcacggat ttgccgcagc ccgattcgcc
    17821 cacgatggcc aaggtctcgc ccgcttccac ggaaaacgat acgccgtcga ccgctcggtt
    17881 cacgccatcc ggcgtccgga agtgtgtctg tagattttcg acttcgagta tcggcattac
    17941 agggcctttt ccatgcgggg atcgagcacg tcgcgcaacc catcgcccag caaattcacc
    18001 gccagcacgg tcagcgacag aaaaaccgca ggaaagataa tgatgtacgg cttgacctgc
    18061 cacaaggcac ggccgtcggc catgatgttg ccccaggatg gcgtggtggt cggaacgccc
    18121 gcgccgatga acgacaggat ggcctccagc accatcgcac tggcgcagat ataggtggcc
    18181 tgcacagtaa tggccgacag cgtattgggc aggacatgcc gcgtaatgat gcgcatggtg
    18241 cgattgccgg aagcgatcgc gccgtcgatg aagggctggt cgcgcagcga cagcaccacg
    18301 ccccggacca gacgggaaat gcgcggtatc tccgagatgg tcagcgcaac gatcacattg
    18361 cccatgctgc cgtgcgacac cgccagcagg gcgatggcca gcagaatggg gggaatcgcc
    18421 atcatcccgt ccatgacgcg catgacgatg ccgtcggccc accgcaaaaa gcccgccacc
    18481 aaccccacgg ccagtcccgc cgccgaggcg aacagcgcca cgcagaaacc cacgatgagc
    18541 gatacgcgcg cgccgtacac aatgcgcgaa taaatgtcgc ggcccagcat atcggtgccg
    18601 aaccaatact gcgccgaggg tggccgggcg cggcgggcag gcgacaaggc attgggatcg
    18661 gcgtgcgcca ggagcggcgc gaagaccgcc atcaacacca gcaacgacaa cagcgccaaa
    18721 gcaaacagca tctgtggatg acgtcggcag aaacccgaga atcccttgcg cacacgaggc
    18781 tctggcagca cgtcaggcag atcaggcgca atcaccaaat tgactggcac ggcgcccatt
    18841 ttcgtctcaa gcggattcaa tatcgtatcc tcgggtcaac cagggtgtag agcagatcga
    18901 tgatcaagtt caccatgaca tacaacagac tgaagaacac caccacgccc tggatgacgg
    18961 ggtagtcgcg ctgcaggatg gcatcgacca ccagccgtcc caggccggga atggcgaaca
    19021 cgctttcggt gaccaccgcg ccgccgatca acagcgctat gcccacgccc accacggtca
    19081 agatggacac cccgcagttg cgcagcgcat gcacgaacag aatgtcgcgc atcgacacgc
    19141 ccttggcgcg cgccgtgcgg atatagtcct gctgcaacac ttccagcatg gccgcgcggc
    19201 tggtccgggc aatcagcgcg acatacatcc cgctgagggt gaacgccggc agaatcagcg
    19261 accgcaccgc ctgccatacg ctttccgaca acggcacgta accctgcacc ggcagccacc
    19321 ccaaccgcag cgcgaacacg tacgccagga tgtagccgat cacgaatacc ggcacggaaa
    19381 agcccaatac cgacatggac atgaccagcg tgtccagtcg cccgccatgc cggaaagccg
    19441 ccaccacgcc catcggcacg ccgaccgcca cggccaggac aatggtcatt accatcaaca
    19501 acaacgtggg ttccaggctt gagccgatca agtgcgccac gggttgcttg ttgaaaacgg
    19561 aatggcccat gtcgccgcgc agcatatgcc cggcccagtc catgaactgc accgcgaagg
    19621 gacgatccag ccccatctcc tggcggatgc gctgcaagtc ctgctgcgtg gcctggtccc
    19681 ccgccaggat ggcggccgga tcgcccggcg ccacgaacag caggctgaag atgaacaccc
    19741 ccaccacgca tagcaccggg atcgtcgcca ggatgcgctt gacaatgtac ttcagcatgg
    19801 cagggccacc tgccggcggc gcgccgatct acgcactgcg aattccccag aaaaaacatg
    19861 ccgacccggg caggtccccg gtcaggttct tgcgcaacgc ggtctggatg gtgaattcgc
    19921 ccagcatgac gacgggcatc atctccagcg cgcggcgctg gctctcctcg aacagatgat
    19981 cctgctcggc ggggtcttgc gcgtccatcc actggctggc gagcttttcg atctcagggt
    20041 cgtcgaacca gccgaaccag ccatccttgc cctgcccgcg cacattgctg ttgagcgacg
    20101 gatcacccac cgacacgctc ggccaggtcg tatggaaaat gctccagccg cccttgtcca
    20161 ccgactcgcg cgacgtgcgg cgctgggtca ccgaccccca gtccatatcc tgcagatcga
    20221 cgttgaagcc catctgcttg agcagatccg ccgtcacgtg gcccagcggg ccgatcatga
    20281 tgttgtcggt ggggttgatg atgacgacct tctcgcccgc gtagccgctt tccttgagca
    20341 gctggcgcgc cttgtccagg ccgccgggct tgccgagcag tgcattcttg acgccgggca
    20401 tgccgcaagg atagttcgag aggcactcct tgtagtcctg ctcgtcgagc cccagcgagt
    20461 tcatatagtc actctggttg acggcctcgt agaccgctcg ccgcaccttg gggttgttga
    20521 acggcgcctg cgcgctgttg aatcgcatca cgacgatcat gccgtacggg tcggacaccc
    20581 gagtccggat attggcattg ctccgcagca cgggcaacag atcgggaagc ggcacttccc
    20641 accagtcgac ctcgccgttc tgcagcgccg aggccgccgt cgccgaatcg ggaatgatgt
    20701 gccattccag gcggtcgaaa tgcaccacct tgccgccgga agtccagtcc gccggttcgg
    20761 aacgcggctt gtagccttcg aatttctcgt acaccgcctt gctgccaggc acgtattcgg
    20821 ccgccacgaa acggaacggc cctgaaccta ccatctcggt cagcggctcc gaggcgctaa
    20881 ggccggccaa tcgctcaggc atcatgaaag cgggagacga atgcggcttg ctcaatacga
    20941 acagcatgcg cgggaagggc ttcttgagct tgatcaccag gctgcggtcg tcggacgtgt
    21001 cgtagtcggc cacggccttg gccaattcct ggcccgaggt atcgcgctgc ccccagcgct
    21061 tcaggctggc ggcgcaatcg cggctgcgca ccggctggcc gtcatggaaa gccaggccgt
    21121 cgcgcagctt gatcgtccag gtcaacccgt ccgcggacac ttgggcgctt tccgccatct
    21181 gcggcttggg ctccagcttg ccgttgacgc cgtagagcgt gtcgaacacg cagtagccgt
    21241 gcgtcacggt gacggcagcc gtcgtcacga caggatcgag aatggtcagg ttggcctgcg
    21301 gcacgaattt cagcacgctc ttgccgcctt cctgggcgaa cgcgaagcgc ggagccattg
    21361 cgctggcccc ggccataccg gcaatgcgaa gaaaatctct gcggttgaac atgatgcttg
    21421 tttcccttgg ggctaggttt ttgtcaattg atcgatgccg tcgcagcctc gcgccggcgc
    21481 atgcggaccg cgggaaagag attcccgtga ttcgcgttag aaaggatcgg caaaattccg
    21541 gtcccgcggc cagaacgggc tcggccggtt gcgccaaggc agcgctccca gaccggccgg
    21601 cgaggcgccc acgctgtcaa gcagcaccca tggcgtccgc gggcccagca agttgcggaa
    21661 attcatgccg ttcttgagca ccaggacatc gcagtcgtcc ggatcgatcc cggcggcgcg
    21721 gaactgctcg tccgcgtatt cgtaagtcgg cttgctggcc accagtacgc gcacgccgcc
    21781 tgcctgcagc accgccgtgg gacccatgtc gcccaccacg ccgccggatg ggccgccgac
    21841 ataggtaaag ctgccgtcgt gcagattgac caccgtcgcc ttgacgggaa ccggctcgcc
    21901 atagcgcggg tcggcgcgaa agcccagcgt ggcgtccagg acggcccccg cgccggcggc
    21961 atgcgcctcg gccgccacct gcgcgtcgac cagcgacact gcgcagcgca ggctgcccgc
    22021 gtgcttgagc agggcagcca gcacgtaagc ggcgtcgccc gaggcgccgg cgccgacaca
    22081 gtcggccgta tcggcaaaga tgaacggacg caccgtggcg cctcgggcct gctcgatggc
    22141 cgactcgacg ctcagcaaag gcaattcgaa ctcttcccgc aaatcccaca tatgctgggc
    22201 aatctgccgg gatacgccct cggccctgcc ggcatcgccg tcggtgatcg ccaacgccac
    22261 attgcccgct tcgtcatcgt ccaaccaggg ttgcacggtg aagtagctgg tcgagaccac
    22321 gccgtcggtt tccaggctgc gggccagctt ctttacgcgc gccagcggcg cctcgtcgta
    22381 acgggtgcag ctgcccgtca ccggcaccag cataggcagg cgagtcatgc gcatgaccgg
    22441 acgcagtttg ccggccagcg tgtcgcgcaa caatgctgcc gcgcgctttc cggtgtcata
    22501 ggcatcgtcg tgtgggtaat tggcatagcc caccagtatg tcggcggtgc gaaccatccg
    22561 cgccgacaca tgggcatgca agtccaggct cgcggcaatg ggcacgccgg agccgacaat
    22621 ctcacgcacg gccgaaagca gctcgccttc cgcatccttc acgccgccgc tgaccatcgc
    22681 gccgtgcaag gccaggtaga ccccgtcgaa cggcccttgc tcccggatac ccgccacgat
    22741 ggcatccgtg gtgcggcggt agaagtcgtc ctccaccttg ccgcccgaac cgccatgggc
    22801 caccaggatg ggcactaggt cgagcccccc ctcttcgcgc aggacctcga ccgcgccggt
    22861 caccgccaga gggcggccct cgatgcgcgc gaacacatcc ccgcccaccg catacagatt
    22921 gaacagcgcg agatcattta tcttgggaga aaaatcgttg ccctcgaact cgaagctggc
    22981 gattgcgact ctctttcgtc ctgaaaccat ggggaactcc tgggtgcgaa tacgacgtgg
    23041 ccgaattatg agtgttcgaa caatcgcgtc gcaatttcgc agcggggacg aggcatgacg
    23101 caaattgacg cactcaggca cgccgcgatc cggcacggtg acgaattacg gcgctggcct
    23161 atgtgcgtca tccctttcgc acttttccca gcttctaggg aaacccctag ctgaagagtc
    23221 ttgatttaca gccttgccca cttgtgccga ccatcgcgga cagcccgtaa ttcgcttcac
    23281 cgcaacgcgc gtcgcggcca atccaccgac ttgccgcccc cctgatgatt acccctattc
    23341 gcagtgccgc tgcgcgcgaa cgcccttgcc gcgtgcgtat cccgcggact ctcgcaccag
    23401 gctgacgcaa gccgcgcccc cgctgcgtca cagtacgtct aggacggggc cggttgcgaa
    23461 attgaaatga cgtgcgatgc cctggagaat ttcgcccgga catcgccacc ctaagcgcat
    23521 ccgccggggc ggcctgattg catcgatggg ccaagcccgc cccaacccaa aattatggag
    23581 aaagcatgat acgcaagctg aaagctgcaa aacccgccca ggtacaagat gccattgccc
    23641 gcgaggtgca gcaggtcgtt gccgggatca tcgctgacgt cacgcaacgc ggcgacgtcg
    23701 cggtgcggga gtactcggtc aagttcgacc gctggaaccc cgaagccttt tgcttgaacg
    23761 acgcgcagat cgccgactgc atcgcgcaac tctccgcgcg cgaactcgac gacatcaagt
    23821 tcgcgcaagc gcaggtccgg cgcttcgccg aagcgcaaaa agccgcgctc accgacatcg
    23881 agatcgaaac catcccgggc gtcgtgctgg gccaccgcaa catccccgtg gattccgtcg
    23941 cctgctacgt gcccggcggc aaatacccgc tgatcgcttc cgcccacatg ggcgtggtca
    24001 ccgccaaggt ggccggcgta ggccgcatcg tggccgtcgc tccgccgttc gaaggcaagc
    24061 ccaatccggc catcgtcgcc gccatgcacc tgggcggcgc cgacgagatc tactgcatcg
    24121 gcggcgtgca agccgtggcg gcgctggcgc tgggcaccga caccttccgc cccgtcgaca
    24181 tgattgtcgg tccgggcaat gcctatgtcg ccgaggccaa gcggcagttg ttcggcaaga
    24241 tcggcatcga cctgatcgcc ggccccaccg agacgctgct gctgaccgac cacacctgcg
    24301 acgcggaaat ggccgccgcc gacctgctcg gccaggctga acatggcgtc aactctcccg
    24361 ccgtgctggt gaccacctcc aagcaactgg ccgaggccct gcccgccgag atcgagcgcc
    24421 agctggcggt cctgcccacc gcggcgatcg ccggtgtcgc atggcaagac tacggcgaga
    24481 tcattctgtg cgacacgctg gaagagatgg tccaggaagg caaccgcatc gcttccgagc
    24541 atgtgcaggt gctgaccgag gatccggaat acttcctgga acacctgacc aactacggcg
    24601 cgctgttcct gggcgcgcgc accaccgttt catatggcga caaggtcatc ggcaccaacc
    24661 acacgctgcc caccatgggt gaggcgcgct ataccggcgg gc
//
LOCUS       DASWPO010000130         6005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_296435, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000130 DASWPO010000000
VERSION     DASWPO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296435"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VGC69_07435"
     CDS             <1..140
                     /locus_tag="VGC69_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase"
                     /protein_id="HEY0295151.1"
                     /translation="RTTACHIDMPMRHCTVALDGRPVVIAGVVQDEAGLAHAAGKTGL
                     K"
     gene            300..1445
                     /locus_tag="VGC69_07440"
     CDS             300..1445
                     /locus_tag="VGC69_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295152.1"
                     /translation="MAEGTIKIGEINSYKSQPVFTDGYKKGMMLAIDEANAAGGVDGK
                     KLELITRDDNANPGDAVRLAEELISRENVDILAGAFYANIGTALADFAKQKKRFMLVT
                     EALSDNIVWSAGNRYTFRLDASTYMLSAGLIDAAVAQHKKRWALVYPNYEFGTAAAAA
                     FKKLMKERQPDVEFVAEQAPPFNKIDAGSVVQAIVDAKPDGIFNALFGADLAKFVREG
                     TTRNLFGDNMPVFSIITGQPEYLEPLRDEIPPNWTVTGYPWYSIDTPAHKAFLAAYQA
                     KYGKENLRFGSIIGYAAIKSIIAGIQAAGGKTDAESMVKAFSGLKFDTPDGPMQFRAI
                     DHQATWGLYVGKIAAKDGQGAMVDYHYVTGDKLLPSDDEVRKMRPAD"
     gene            1447..3357
                     /locus_tag="VGC69_07445"
     CDS             1447..3357
                     /locus_tag="VGC69_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295153.1"
                     /translation="MNASALLSQLLNGLAEASSLFLVAVGLSLIFGVTRVVNFAHGSF
                     YMLGIYLAYSLVEKFGVGPSGAAGYWCSLVLAAVLAGLIGGIVEIVLLRRIYRAPELF
                     QLLATFALVLVINDAVLWLFGPEELLGRRAPGLSGAIQVLGRAYPSYNLFLIVIGPIV
                     LALLWLLLTRTRWGTLVRAATQDREMLGALGINQAWLFTGVFALGALLAGLGGAVQLP
                     REPASLTMDLRAIGDAFVVVVVGGMGSIPGAYVAALLIAEIKALCVGLGVVHFLGVSF
                     SFTKLTLVVEFLVMALVLVFRPWGLMGRPQAASRNTGPVEAPLRPGTPAFKALVTVVV
                     IILAVLPLLSTWLPYATVLTQDVLTAVLFAVSLHFIMGPGGMHSFGHAAYYGLGAYGA
                     GILLKSLTMPMPLSLALAPLFAGAGALVFGWFCVRLSGVYLAMLTLAFSQIVWSVVFQ
                     WDEVTGGSNGMIGVWPPDWLGGNAYYYLTLVLVVLAIFVLRRMLFAPFGYALRAGRDS
                     ALRADAVGIDVKRVQWAAFVIAGLAAGLAGAIFVFSKGGISPDSMNVGKSVDGLVMVL
                     LGGVQTLLGPVVGAAVFNVLQDYIIRATDYWRALFGGIILLLVLAFPQGIVGFARQVA
                     ERWGARSAKGTT"
     gene            3354..4139
                     /locus_tag="VGC69_07450"
     CDS             3354..4139
                     /locus_tag="VGC69_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295154.1"
                     /translation="MTALLEVRNLSKAFGGVKAVDGIDFDIAEGELLALIGPNGAGKS
                     TTFNMVGGQLSATTGTVMFDGVDLVGMKPRDIWRRGVGRTFQIAATFASLTVAENVQM
                     ALLSHHGRVFGLWKPAAAQFRGEAVALLAQVGMDAQADRPCSELAYGDVKRVELAMGL
                     ANDPKLLLMDEPTAGMAPKERNDLMALTKRLVVERRMAVLFTEHSMDVVFAYADRMIV
                     LARGRLIAEGDAQTIRNHPKVQEVYFGSGKTFEKGKAMQGALA"
     gene            4136..4912
                     /locus_tag="VGC69_07455"
     CDS             4136..4912
                     /locus_tag="VGC69_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295155.1"
                     /translation="MSGAALDTPMFGAATPVQPPLLEVGGLNAWYGSAHILFDVSLDV
                     RRGEVVALMGRNGAGKSTTFKAIMGLLGKSQGQVKFLGKPIMGLQPYEVARLGLGFVP
                     EDRRIFTDLTVTENLDVGRQPPRLWPDGSESLTWTPEALYELFPNLGAMQDRPGGNMS
                     GGEQQMLTVARTLMGNPFLVLLDEPSEGVAPVIVEQMAHMILQLKAKGASILLSEQNM
                     HFAELVSDRAYVLEKGQIKYEGSMAELAANDEVRRAYLAM"
     gene            4935..5954
                     /locus_tag="VGC69_07460"
     CDS             4935..5954
                     /locus_tag="VGC69_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0295156.1"
                     /translation="MKAFYHPDQVLHHPRSYYSRGKMREPQEVPGRVQALADTARKLG
                     MDVQQPADAGAGPISAVHTLDYLAFLQSAWREWKDLPEDWGDEVLSNVFVRSPNPMRG
                     ILAKAARYLADGSAPVGEQTWRSAYWAAQCAIAGAQALIDGDPSAYAICRPPGHHARV
                     DAAGGFCYLNNAAIAAQHLRGKFKRVVILDTDMHHGQGIQEIFWERNDVFYISIHGDP
                     TNFYPAVEGFEDERGAGAGFGYNLNIPMPHGSSQEVFMKSLDQAMDAIKLFQPDALVH
                     CLGFDVFEKDPQHKVSVSSEGFEILGRRIAACGVPTLIVQEGGYHIETLGDNATRFFA
                     GLGRS"
BASE COUNT          951 a         1987 c         2013 g         1054 t
ORIGIN      
        1 agcgcaccac agcctgccac atcgacatgc ccatgcggca ttgcacggtg gcgctcgacg
       61 gcaggcccgt cgtgatcgcg ggcgtggtcc aggacgaggc cgggctggcc catgccgccg
      121 gcaagacggg gctgaagtga cccgccaggt ttttgagcaa tggtttgaac aaatcgtttg
      181 catgcgttgt gtggttcgat gagtcgtcaa tatccaaggg gaacacgttc atgagtatcc
      241 gtatcaagca gtcgatcctg gcattgggcc tggccgcggt cgccgcggcg caaaccgcga
      301 tggccgaggg caccatcaag atcggcgaga tcaacagcta caagtcccag ccggttttca
      361 cggacggcta caagaagggc atgatgctgg ccatcgacga agccaacgcc gccggcggcg
      421 tcgacggcaa gaagctcgaa ctcatcaccc gcgacgacaa cgccaacccc ggcgacgccg
      481 tgcggctggc cgaagagctc atctcgcgcg aaaacgtcga catcctggcg ggcgccttct
      541 atgccaacat cggcacggcc ctggccgatt tcgccaagca gaagaagcgc ttcatgctgg
      601 tcaccgaggc gctctccgac aacatcgtgt ggtccgcggg caaccgctac accttccgcc
      661 tggatgcctc cacctacatg ctgagcgcgg gcctgatcga cgcggccgtc gcccagcaca
      721 agaagcgctg ggcgctggtc taccccaact atgagttcgg cacggccgcc gccgccgcct
      781 tcaagaaact catgaaggaa cgccagcccg acgtcgaatt cgtcgccgaa caggcgccgc
      841 ccttcaacaa gatcgatgcc ggcagcgtgg tgcaggccat cgtcgatgcc aagcccgacg
      901 gcatcttcaa cgcgctgttc ggcgccgacc tggccaagtt cgtgcgcgaa ggcacgaccc
      961 gcaacctgtt cggcgacaac atgccggtgt tttccatcat taccggccag cccgaatacc
     1021 tggagccgct gcgtgacgaa attccgccca actggacggt gaccggctac ccctggtaca
     1081 gcatcgatac gccggcgcac aaggctttcc tcgccgccta ccaggccaag tacggcaagg
     1141 aaaacctgcg cttcggctcg atcatcggct atgccgccat caagtccatc atcgccggca
     1201 tccaggcggc gggcggcaag accgacgccg aatccatggt caaggccttc agcggcctga
     1261 agttcgacac gccggacggc ccgatgcaat tccgcgccat cgaccatcag gccacctggg
     1321 gcctgtatgt gggcaagatc gccgccaagg acggccaggg cgccatggtc gactaccact
     1381 acgtgaccgg cgacaagctc ctgccgtccg acgacgaagt gcgcaagatg cgtccggccg
     1441 actgagatga atgcatccgc actgctgtcc cagttgctca acgggctggc cgaggcgtcg
     1501 tcgcttttcc tggtggcggt cggcctgtcc ttgatcttcg gggtcacgcg cgtcgtcaat
     1561 ttcgcccacg gctctttcta catgctgggt atctatctgg cgtattcgct ggtggaaaaa
     1621 ttcggcgtgg ggccgtcggg cgccgcgggc tattggtgca gcctggtgct ggccgcggtg
     1681 cttgccgggc tcatcggcgg catcgtggag atcgtgctgc tgcggcgcat ctaccgggca
     1741 cccgagctgt tccagctatt ggccaccttt gcgctggtgc tggtcatcaa cgacgcggtg
     1801 ctgtggctgt tcggacctga agagctgctg ggccggcggg cgccgggcct gagcggggcc
     1861 atccaggtcc tcgggcgcgc ctatcccagc tacaacctgt tcctgatcgt catcggcccg
     1921 atcgtgctgg ccctgctgtg gctgctgctg acgcgcacgc gctgggggac gctcgtgcgc
     1981 gccgccacgc aagaccgtga aatgctgggc gccctgggca tcaaccaggc ctggctgttc
     2041 accggcgtgt tcgccctggg cgcgctcctg gcggggctgg gcggcgccgt gcagctgccg
     2101 cgcgagccgg ccagcctgac gatggacctg cgcgccatcg gcgacgcctt cgtggtggtg
     2161 gtggttggcg gcatgggttc catcccgggc gcctacgtcg cggcgctcct catcgccgaa
     2221 atcaaggcgc tgtgcgtggg cctgggcgtg gtgcacttcc tgggtgtttc gttttcgttc
     2281 accaagctga cgctggtggt cgagttcctg gtgatggcgc tggtgctggt cttccggccc
     2341 tggggcctga tgggccgccc gcaggcggcc agccgcaaca ccggcccggt cgaggcgccg
     2401 ctgcgtcccg gcacgcccgc cttcaaggcg ctggtgacgg tcgtggtgat catcctggcg
     2461 gtgctgccgc tgctgtcgac ctggctgccc tatgccacgg tgctcacgca ggacgtgctg
     2521 accgccgtgc tgttcgccgt gagcctgcac ttcatcatgg ggcccggcgg catgcactcc
     2581 ttcggccatg ccgcctacta cggcctgggc gcctacggcg ccggcatcct gctcaagtcg
     2641 ctgaccatgc ccatgccgct gtcgctggcc ctggcgcccc tgttcgccgg cgcgggggcg
     2701 ctggtgttcg gctggttctg cgtgcgcctg tcgggcgtct acctcgccat gctcacgctg
     2761 gccttctcgc agatcgtgtg gtcggtggtg ttccagtggg acgaagtcac aggcggctcc
     2821 aacggcatga tcggcgtgtg gccgcccgac tggctgggcg gcaatgccta ttactacctg
     2881 acgctggtgc tggtggtgct ggccatcttc gtgctgcggc gcatgttgtt cgcgcccttc
     2941 ggctatgcct tgcgcgccgg ccgggactcg gccctgcgcg ccgacgcggt gggtatcgac
     3001 gtcaagcgcg tgcaatgggc cgccttcgtc atcgccggcc tcgccgccgg cctggcgggc
     3061 gcgatcttcg tgttttccaa gggcggcatt tcgccggatt ccatgaacgt gggcaagtcg
     3121 gtcgacggcc tggtgatggt gctgctgggc ggcgtgcaga cgctgctggg cccggtggtg
     3181 ggcgcggcgg tgttcaacgt gctgcaggac tacatcatcc gcgccacgga ctactggcgc
     3241 gccctgttcg gcggcatcat cctgctgctg gtcctggcct tcccgcaagg catcgtgggc
     3301 ttcgcccgcc aggtggccga gcgctggggg gcgcgtagcg ccaaggggac cacatgacgg
     3361 ccctgctgga agtccgcaac ctgagcaagg ctttcggcgg cgtgaaggct gtcgacggca
     3421 tcgatttcga catcgccgag ggcgagctgc tggcgctcat cggccccaac ggcgcgggca
     3481 agtccaccac cttcaatatg gtgggcggac aactgtcggc caccaccggg acggtcatgt
     3541 tcgacggcgt cgacctggtc ggcatgaagc cgcgcgacat ctggcggcgc ggcgtgggcc
     3601 gcaccttcca gatcgccgcc acctttgcct cgctgacggt ggccgagaac gtgcagatgg
     3661 ccctgttgtc gcaccacggg cgggtgttcg gcctgtggaa gccggcggcg gcgcagttcc
     3721 gcggcgaggc cgtggccctg ctggcgcagg tgggcatgga cgcccaggcc gaccggccct
     3781 gcagcgagct ggcctacggc gacgtcaagc gcgtggagct ggccatgggg ctggccaacg
     3841 atcccaagct gctgctcatg gacgagccca cggcgggcat ggcgcccaag gagcgcaacg
     3901 acctcatggc gctgaccaag cgcctggtcg tcgagcggcg catggccgtg ctgttcaccg
     3961 agcacagcat ggacgtggtg ttcgcctatg ccgaccgcat gatcgtgctg gcgcgcggcc
     4021 ggctgatcgc cgaaggcgat gcgcagacca tccgcaacca tcccaaggtg caggaagtgt
     4081 atttcggttc tggcaagaca ttcgagaagg gcaaggccat gcagggagcc ctggcatgag
     4141 cggcgcagcg ctcgatacac ccatgttcgg cgccgccacg cccgtgcagc cgccgctcct
     4201 ggaagtcggc gggctgaacg cctggtacgg ttcggcccac atcctgttcg acgtgtcgct
     4261 cgatgtgcgg cgcggcgagg tcgtggccct gatggggcgc aacggggcgg gcaagtccac
     4321 caccttcaag gccatcatgg ggctgctggg caagagccag ggacaggtga agttcctggg
     4381 caagcccatc atgggcctgc agccctacga ggtggcgcgc ctgggcctgg gtttcgtgcc
     4441 cgaagaccgg cgcatcttca ccgacctcac cgtgaccgaa aacctggacg tcggccgcca
     4501 gccgccgcgg ctgtggcccg acggcagcga gtcgctcacc tggacgcccg aggccctcta
     4561 cgagttgttt cccaacctgg gcgccatgca ggaccgtccc ggcggcaata tgagcggcgg
     4621 cgagcagcag atgctcaccg ttgcccgcac gctgatgggc aaccctttcc tggtgctgct
     4681 cgacgagcct tccgaaggcg tggcgccggt gatcgtcgaa cagatggcgc acatgatcct
     4741 gcagctcaag gccaagggtg caagcatcct gctatcggag cagaatatgc acttcgccga
     4801 actggtgtcc gaccgggcct acgtgctgga aaaaggccag atcaagtacg agggcagcat
     4861 ggccgagctg gctgccaacg atgaagtgcg gcgtgcctat ctcgccatgt gatcccccat
     4921 ccgtcagagt ctccatgaaa gccttctacc atcccgacca ggtattgcat cacccccgtt
     4981 cttattattc gcgcggcaag atgcgcgagc cgcaggaagt ccccggccgc gtgcaggcgc
     5041 tggccgacac ggcccgcaag ctgggcatgg acgtgcagca gccggcggat gccggcgccg
     5101 gcccgatctc ggccgtgcac acgctggact acctggcctt tctgcaaagc gcctggcgcg
     5161 aatggaaaga cctgcccgag gactggggcg acgaggtgct gtccaatgtg ttcgtgcgct
     5221 cgcccaatcc catgcgcggc atcctcgcca aggcggcccg ctacctggcc gacggcagcg
     5281 cgccggtggg cgagcagacc tggcgctcgg cctactgggc cgcgcagtgc gccatcgccg
     5341 gcgcgcaggc cctgatcgac ggcgacccgt cggcctatgc catctgccgc ccgcccggcc
     5401 accatgcgcg cgtcgatgcc gcgggcggct tctgctacct gaacaacgca gccatcgccg
     5461 cccagcactt gcgcggcaag ttcaagcgcg tcgtcattct cgataccgac atgcaccacg
     5521 gccagggcat ccaggagatc ttctgggagc gcaatgacgt cttctacatc tccatccacg
     5581 gcgaccccac caacttctat cccgcggtcg agggtttcga ggacgagcgc ggcgcggggg
     5641 cagggttcgg ctacaacctg aacattccca tgccgcacgg cagcagccag gaagtgttca
     5701 tgaagagcct ggaccaggcc atggacgcca tcaagctgtt ccagcccgat gcgctggtgc
     5761 actgtctggg ctttgacgtg ttcgagaagg acccgcagca caaggtgtcg gtgagttccg
     5821 aggggttcga gatcctgggc cgcaggattg ccgcttgcgg cgtgccgacg ctgatcgtgc
     5881 aggagggcgg gtatcatatc gagacgttgg gtgataacgc tacgcggttt tttgctggac
     5941 ttgggcgtag ttgagttctt gagccgcatc gtcgccgggg aagagggggc cggctgcatc
     6001 gcggc
//
LOCUS       DASWPO010000131         4538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1903153, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000131 DASWPO010000000
VERSION     DASWPO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903153"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VGC69_07465"
     CDS             complement(<1..312)
                     /locus_tag="VGC69_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEY0295157.1"
                     /translation="MAVTEHHSLAAGQKHDSVPRLKKLARTLTSRLRLGLFGRTFLLL
                     AALMLVSLGAWLQVFFSMELGPRANQMAQRVITAVNITRTALVYSHADERSKLLLDLA
                     TN"
     gene            complement(299..1033)
                     /gene="ompR"
                     /locus_tag="VGC69_07470"
     CDS             complement(299..1033)
                     /gene="ompR"
                     /locus_tag="VGC69_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEY0295158.1"
                     /translation="MNTQNTTPIRKILVVDDDPRLRDLLRRYLSEQGFNVFVAEDAKE
                     MGKLWQREHFDLLVLDLMLPGEDGLSICRRLRGGHDNTPIIMLTAKAEEIDRIVGLEM
                     GADDYLSKPFNPRELLARINAILRRRGTEEHPGAPSQENESIAFGPYVLNLSTRTLTK
                     DNEPVPITTGEFSVLKVFARHPKIPLSRDKLMELARGREYEAFDRSLDVQISRLRKLI
                     EPNPSKPVFIQTVWGLGYVFVPDGGN"
     gene            1176..2477
                     /gene="tsaB"
                     /locus_tag="VGC69_07475"
     CDS             1176..2477
                     /gene="tsaB"
                     /locus_tag="VGC69_07475"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY0295159.1"
                     /translation="MKLNLLAFETSSSRCGVALLRQDGGPPELAQLEHDGAQEHAERL
                     LPMAGELLARAGLAPAALDAVAFGQGPGGFTGLRVACGVAQGIALGLGLPVVPVVSHL
                     AVAEDAQAGPDDAVLVALDARMEEVYLAVYMRASAQAGVQDSIQDSGGGRAWRVLQEP
                     LLIAAAEVVPWAQHHLPAWSRQAGRALSLLLAGDAWRAYPAISEAGLRQAPLGRPRAA
                     TVARLARQAWLRGETLPPEEAAPLYVRDKVAFTTAERERGQGGNPRASSALPAAEAYV
                     LSAMSLGDLDEVAALEAQVQDFPWTRGNFDDALRSGYPACVLRLGAQLAGFCVMMPAP
                     DVAHLLVIAVARDRQRHGLGHRLVDWCHAQAVASGAEGILLEVRPSNENARRFYERLG
                     FRQIGVRKGYYPAAAGREDALVLRRDVLAQGRRDVPPGGGA"
     gene            2474..3319
                     /locus_tag="VGC69_07480"
     CDS             2474..3319
                     /locus_tag="VGC69_07480"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0295160.1"
                     /translation="MMTAGGAPRVNPLQRLWLREIGIEKTWLRQDEPPKPAAVAARPA
                     APSAAPQAEAAAPRPVAAAPSRPAPARAPAPAAAEPAAAAPREVDPEQAGRVARADLG
                     TLQEMVTGCSACGLCQGRRQAVFGMGAAAPRWMVVGEAPGEQEDRQGLPFVGRSGQLL
                     DAMLAAVGMSRERDVFIGNVIKCRPPGNRNPTPEEIAACSPYLMRQIELLKPERILVL
                     GRFAAQTLLDTDATIGSLRGRVHTVRVGGREIPVVVSYHPAYLLRSPSEKARSWQDLQ
                     LAART"
     gene            complement(3350..>4538)
                     /gene="lplT"
                     /locus_tag="VGC69_07485"
     CDS             complement(3350..>4538)
                     /gene="lplT"
                     /locus_tag="VGC69_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY0295161.1"
                     /translation="PLMKWSFALAYVVLAAFVGAFADSFPKGWVMFLTNALKVGGCLL
                     MFCYASLGLHFLHRSYLVCLAYALVGIGAAAYSPAKYGIVTEMLPPQLLVKGNSWIEG
                     LTVLSIIIGTMLGGLLISPRVSDVLLHHSLVSKLVRTPAEGAILVIAAIYVMAALCNL
                     LIPKTHVRYPRQQKNPFKLVRVFGGYVGVLWRDKLGQISLAVTTLFWGAGATLQLVVI
                     EWGKSHLGYRLDQASMLMGVAAAGTVVGSILAGRISLSRALCVLPVGALMGLVVLLMP
                     LAYSTWSVYLLLLVTGALAGFFVVPMNALLQHRGHVLLSAGHSIAVQNFNEQINILLM
                     VAAYTLLLWLKLPINQIIVLFGLIVVVLMLIFMAWSRRNMKAHPELQQQIGLEGHGRA
                     MDE"
BASE COUNT          723 a         1436 c         1673 g          706 t
ORIGIN      
        1 gttggtggcc aggtcgagca gcagcttgct gcgctcgtcg gcatgggagt agaccagggc
       61 cgtgcgcgtg atgttgacag ccgtgatgac gcgctgcgcc atctggttgg cgcgcgggcc
      121 cagctccatg ctgaaaaaca cttgcagcca ggcccccagg ctgaccagca tgagggcggc
      181 aagcagcagg aaagtacggc cgaacagccc cagcctcaag cgtgaggtga gggttctggc
      241 tagttttttc aggcggggca cggaatcatg cttctgcccg gcggccaggc tgtgatgctc
      301 agttaccgcc atccgggacg aatacgtagc ccaggcccca gaccgtctgg atgaagaccg
      361 gcttggaggg gttgggttcg atcagcttgc gcaggcggga aatctgcacg tcgaggctgc
      421 ggtcgaaggc ttcgtactcg cggccgcgcg ccagttccat gagcttgtcg cgcgagagcg
      481 ggatcttggg gtggcgggca aagactttga gcacggagaa ttcgcccgtg gtgatgggga
      541 ccggctcgtt gtccttggtg agcgtgcggg tggacaggtt gagcacatag ggcccgaagg
      601 cgatggactc gttttcctgg ctgggcgcgc cggggtgttc ttccgtgccg cggcggcgca
      661 ggatggcatt gatgcgggcc agcagttcgc gcgggttgaa aggcttggac aggtaatcgt
      721 cagcacccat ttcgaggccg acgatacggt cgatttcttc ggccttggcc gtgagcatga
      781 tgatgggcgt gttgtcgtgc ccgccgcgca ggcgccggca gatcgacagg ccatcttcgc
      841 caggcagcat caggtcgagc accagcaggt cgaagtgctc tcgctgccac agcttgccca
      901 tttccttggc gtcttcggcg acgaatacgt tgaaaccctg ttccgacaga taacggcgca
      961 gcaggtcacg cagccgggga tcgtcgtcga cgacgaggat cttgcggatg ggagtggtgt
     1021 tttgagtatt catggcggca aatgtaacag cgggacgtgg caccgactag tgagtattca
     1081 taacaagata ttacgttttg catattgcca tgaaaacccg ggcacaggga tagaacggtg
     1141 cgaacacgga tcgcgattcc gtacaattct cctccatgaa gctgaattta ctggcctttg
     1201 aaacttcttc ctcgcggtgc ggcgtggccc tgctgcggca ggacggcggc ccgcccgagc
     1261 tggcgcagct cgagcacgac ggagcccagg agcacgccga gcgcctgctg cccatggcgg
     1321 gcgaactgct ggcgcgcgca ggcctggcgc cggctgccct ggatgccgtg gccttcggcc
     1381 aggggccggg cggcttcacg ggcctgcggg tggcctgcgg cgtggcccag ggcatcgcgc
     1441 tggggctggg cctgccggtg gtgccggtcg tctcccatct ggcggtggcc gaggacgccc
     1501 aggccgggcc ggacgacgcg gtcctggtgg cgctcgatgc ccgcatggaa gaggtctatc
     1561 tggcggtcta tatgcgggcg tccgcccagg cgggcgttca agattcgata caagattccg
     1621 ggggcgggag ggcctggcgc gtgctgcagg agcccctgct gatcgccgcc gccgaggtcg
     1681 tgccctgggc gcagcaccat ctgccggcct ggtcgcggca ggccggccgc gcgctgtcgc
     1741 tcctcctggc gggcgatgcc tggcgggctt atcccgccat atccgaggca gggctgcggc
     1801 aggcgcccct ggggcggccg cgcgccgcca cggtggcgcg cctggcccgg caggcctggc
     1861 tgcgcggcga gacgctgccg cccgaagagg cggcgccgct ctatgtgcgc gacaaggtgg
     1921 ctttcaccac ggccgagcgg gaacgggggc agggcggcaa tccgcgcgcc agcagtgcgc
     1981 tgcccgccgc cgaggcctat gtcctgagcg ccatgtccct tggcgacctg gacgaggtgg
     2041 cggccctgga ggcgcaggtg caggactttc cctggacccg gggcaatttc gacgatgccc
     2101 tgaggtccgg ctatccggcc tgcgtgctgc ggctcggcgc gcagctggcg ggcttttgcg
     2161 tgatgatgcc ggcgcccgac gtggcgcacc tgctggtgat cgccgtggcc cgggaccggc
     2221 agcggcatgg gctgggccat cgcctggtcg actggtgcca tgcccaggcc gtggccagcg
     2281 gcgccgaggg catcttgctg gaggtccggc cgtcgaacga gaacgcgcgc cgcttctacg
     2341 agcggctggg ctttcggcag atcggcgtgc gcaaggggta ttaccccgcc gccgccggcc
     2401 gcgaggacgc gttggtactg cgccgcgatg ttctggcgca ggggcgccgc gatgtgccgc
     2461 caggcggggg ggcatgatga cggccggggg ggcgcctcgc gtcaatccgc tgcagcgcct
     2521 gtggctgcgc gagatcggca tcgaaaagac ctggctgcgg caggacgagc cgcccaagcc
     2581 cgcggcggtt gccgcgcggc ctgcggcccc gagcgccgcc ccgcaggccg aggccgccgc
     2641 gccccgcccc gtggcggccg cgccgtccag gccggcgccc gcgcgcgcgc cggctcccgc
     2701 agccgccgaa cctgctgcgg ccgcgccgcg cgaggtggat cccgaacagg ccgggcgcgt
     2761 ggcccgggcc gacctgggca ccttgcagga gatggtgacc ggctgctcgg cctgcggcct
     2821 gtgccagggc cggcgccagg ccgtcttcgg catgggggcg gcggcaccgc gctggatggt
     2881 ggtgggcgaa gcgccgggcg aacaggaaga ccgccagggc ctgcctttcg tggggcgttc
     2941 ggggcaattg ctcgatgcca tgctggcggc cgtgggcatg agccgcgagc gcgatgtgtt
     3001 catcggcaat gtgatcaagt gccgtccgcc gggcaaccgc aatcccacgc ccgaggaaat
     3061 cgcggcctgc agcccttacc tgatgcggca gatcgaactg ctcaagccgg aacgcatcct
     3121 ggtgctgggg cgctttgccg cccagacctt gctcgatacc gatgccacca tcggcagcct
     3181 gcgcggacgc gtgcacaccg tgcgggtcgg tgggcgcgag attccggtgg tggtgagcta
     3241 tcacccggcc tacctgttgc gcagcccgtc ggaaaaggcg cgcagctggc aggacctgca
     3301 actggccgcc aggacctagg ccccgggcag cgggcctttt cggtccagcc tattcgtcca
     3361 tggcgcggcc gtggccttcc aggccgatct gctgctgcag ttcgggatgg gccttcatgt
     3421 tgcggcggct ccaggccatg aagatcagca tcagcacgac gacgatcagg ccgaagagaa
     3481 cgatgatctg gttgatgggg agcttgagcc acagcagcag cgtataggcg gccaccatga
     3541 gcaggatgtt gatctgctcg ttgaagttct gcacggcgat cgagtgcccg gcggaaagca
     3601 gcacgtgccc gcggtgctgc agcagcgcgt tcatgggcac cacgaaaaag ccggccagcg
     3661 cgccggtgac cagcagcagc aggtagacgc tccaggtgga ataggccagg ggcatgagca
     3721 gcaccaccag gcccatcagg gcgccgaccg gcagcacgca cagggcccgg gacagggaga
     3781 tgcgcccggc caggatggag cccacgacgg tgccggccgc ggccacgccc atgagcatgg
     3841 aagcctggtc gagccggtag cccagatggc tcttgcccca ttcgatgacg acgagctgca
     3901 gcgtggcgcc ggcgccccag aacagcgtgg tcaccgccag cgagatctgc cccagcttgt
     3961 cgcgccacag caccccgaca tacccgccga acacccgcac cagcttgaag gggttcttct
     4021 gctggcgggg gtagcgcaca tgggtcttgg ggatcagcag gttgcacagg gcggccatca
     4081 cgtagatcgc ggcgatcacc aggatggcgc cttcggcggg cgtgcgcacc agcttgctga
     4141 cgagggagtg gtgcagcagc acgtcggaga cgcgcggcga gatgagcagc ccgcccagca
     4201 tggtgccgat gatgatggac agcacggtga ggccctcgat ccagctgttg cccttgacca
     4261 gcagttgcgg gggcagcatt tccgtgacga tgccgtactt ggcgggcgag taggcggccg
     4321 cgccgatgcc caccagggca taggccaggc agaccaggta ggaccggtgc aggaaatgca
     4381 ggcccaggct ggcgtagcag aacatgagca ggcagccgcc caccttgagc gcgttggtca
     4441 ggaacatgac ccagcccttg gggaaggagt cggcgaacgc gcccacgaag gcggccagga
     4501 cgacataggc cagggcgaag gaccacttca tgagaggc
//
LOCUS       DASWPO010000132         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2528682, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000132 DASWPO010000000
VERSION     DASWPO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528682"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1041
                     /locus_tag="VGC69_07490"
     CDS             169..1041
                     /locus_tag="VGC69_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0295162.1"
                     /translation="MILLQLIYSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALML
                     GALVGLTLVGLGVNYWVMIPIACAFGFLQGAFVERIGVKPALKTRSEFGWIMATIALG
                     IIFRNVAENIWGHDALRFPSPLPEAPIKLLGANVLPMELLVVGGALAMMLLVELFNRK
                     SIYGKAFVATSTDRPAAGLMGIDTNLVVTFSYAMSSLTAAFAGVLVAPLTLTGAAMGA
                     VLGLKAFAVAIIGGLSSGMGVVVGGLILGIAETTTGFYLSTGYKDVPGLVLLLLVLAF
                     KPAGLFGKTAIKKV"
     gene            1081..3015
                     /locus_tag="VGC69_07495"
     CDS             1081..3015
                     /locus_tag="VGC69_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY0295163.1"
                     /translation="MTPLRAVLALAAVLCLAAAPLTIGTYYLHLVETILIYAILLFGL
                     DIVVGYTGQVSLGHAGLFGIGSYVVGVLFLKLHLPFWISLPSAIVVAAAFGAVLALPA
                     LRVTGPYLAMVTLAFGTIIQILINEMDFLTAGPLGIKIPRPNFAGHPFNRQEYFWLVS
                     ALLALSLIVVHRVLKSHLGRSFEALRDSPIASDCMGVSVYRHKVYAFVISAGFAGLAG
                     GLYSYSEQYISPNTYNFELTVLFLLAIIMGGRKTRSGAILGAAIIVLLPKMLDDIDTF
                     RLIALAVAVVVNAGCALAIARGRAPARRLAVPMAGTLLLAAVSYWLDSVTDWRLTIFG
                     VMILFVVYYLPDGIVGFVRGQLLPARRAAPADLDAGLPPAEAVPPAAHAGADGAELLK
                     VDGVLMQFGGLKALNKVDLRVKRGTIHGLIGPNGSGKSTMMNVLTGIYVPTAGGVTFS
                     GNSLVGLPPARIALSGVARTFQNVQLFGEMNALENILVGLHHTFHTGFAGVALRTPRW
                     RREENAARARALALLEFVGLRNLALEEARNLPYGKQRLLEIARALALNPSLLLLDEPA
                     AGLTAPDIAELLAIIRKVRDHGITLILIEHHMDVVMSLCDTVTVLDFGQKIAEGAPAA
                     VQADEKVIAAYLGGTVAEAA"
     gene            3016..>3521
                     /locus_tag="VGC69_07500"
     CDS             3016..>3521
                     /locus_tag="VGC69_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295164.1"
                     /translation="MLQVDNLVAGYGKVQVLHGLSMSVPQGQLVTLIGSNGAGKTTTL
                     RALSGMIRPDSGSITLGGQQIAGLPSYTIARRGLAHSPEARRVFATLSVADNLVMGAL
                     PRLTGSRPRGDVAADLDRMFQLFPRLQERRTQLAGTLSGGEQQMLAMARALMLNPDVL
                     LLDEPSMGL"
BASE COUNT          513 a         1310 c         1092 g          606 t
ORIGIN      
        1 accctggggg cgcctctgtg acgggcagcc cgaagggccg agggcccgtc gtcttgggcc
       61 cgagcccggc acagcgaagc ccccagggtg tcgccccgcc ggccgatgga cggacccaga
      121 acgcaaccgc ttcccaacaa cccaacatac aagcgacagg aaccctagat gatccttctg
      181 caactcatct acagcggcat cgccctgggc atgatctacg cggtcatcgc cttcggctac
      241 cagctcacct tcgccacgtc gggcacgctg aacttcggcc agggcgaagc cctgatgctg
      301 ggcgccctgg tcggcctcac cctggtgggc ctgggcgtga actactgggt gatgattccc
      361 atcgcctgcg ccttcggctt cctgcaaggc gccttcgtcg aacgcatcgg cgtcaagccc
      421 gcgctcaaga cgcgctcgga attcggctgg atcatggcca cgatcgcgct gggcatcatc
      481 tttcgcaacg tggccgaaaa catctggggc cacgacgccc tgcgctttcc ctcgccgctg
      541 cccgaagccc ccatcaagct tctgggcgcc aacgtgctgc ccatggaact gctggtcgtg
      601 ggcggcgcgc tggccatgat gctgctggtc gagctcttca accgcaagtc catctacggc
      661 aaggccttcg tggccacctc caccgaccgc cccgcggccg gcctgatggg tatcgacacc
      721 aacctggtgg tcaccttttc ctacgccatg tcctcgctca cggccgcctt cgccggcgtg
      781 ctggtggcgc cgctcacgct caccggcgcc gccatgggcg cggtgctggg cctgaaggcc
      841 ttcgcggtgg ccatcatcgg cggcctgtcc agcggcatgg gcgtggtggt gggcggcctg
      901 atcctgggca tcgccgaaac caccacgggc ttttatcttt ccaccggata caaggatgtg
      961 cccggcctgg tgctgctgct gctcgtgctg gccttcaagc ccgccggcct cttcggcaag
     1021 accgccatca agaaagtctg acggcacacc tcctacagga catcctgcat gacatccacg
     1081 atgacacccc tgcgcgccgt gctggccctc gcggccgtgc tgtgcctggc cgcagccccc
     1141 ctgaccatcg gcacctatta cctgcacctg gtcgaaacca tcctgatcta tgccatcctg
     1201 ctcttcggac tggacatcgt ggtgggctac acgggccagg tatccctggg ccacgccggc
     1261 ctgttcggca tcggctccta cgtggtgggc gtgctgttcc tcaagctgca cctgcctttc
     1321 tggatctcgc tgccctcggc catcgtcgtc gcggcggcgt tcggcgcggt gctggccctg
     1381 cccgcgctgc gcgtcaccgg cccctatctg gccatggtga cgctggcctt cggcaccatc
     1441 atccagatcc tcatcaacga gatggacttt ctcacggccg gccccctggg catcaagatt
     1501 ccgcggccca atttcgcggg ccatccgttc aaccgccagg aatacttctg gctggtttcg
     1561 gcgctcctgg cgctgtcgct catcgtggtg caccgcgtgc tcaagtcgca tctcgggcgg
     1621 tcgttcgagg cgctgcgcga cagccccatc gcctccgact gcatgggcgt ctcggtgtac
     1681 cggcacaaag tctatgcctt cgtcatcagc gccggcttcg ccggcctggc gggcgggctg
     1741 tactcctact ccgagcagta catctcgccc aacacctaca acttcgaact gaccgtgctg
     1801 ttcctgctgg ccatcatcat gggcgggcgc aagacgcgca gcggcgccat cctgggcgcg
     1861 gccatcatcg tgctgctgcc caagatgctc gacgacatcg acaccttccg cctgatcgcg
     1921 ctggccgtgg ccgtcgtggt gaacgcgggc tgcgccctgg ccatcgcgcg cggccgggcg
     1981 cccgcccgcc gcctggccgt gcccatggcc ggcaccctcc tgctggcggc cgtctcgtat
     2041 tggctggact cggtcaccga ctggcgcctg accatcttcg gcgtgatgat cctcttcgtc
     2101 gtgtattacc tgcccgacgg catcgtgggc ttcgtgcgcg gccagctgct gccggcgcgc
     2161 cgcgccgcgc cggcggacct ggacgcgggc ctgccgcccg ccgaggccgt gccgcccgcc
     2221 gcgcacgcgg gcgccgatgg cgccgagctg ctcaaggtcg acggcgtgct gatgcaattc
     2281 ggcggattga aggcgctcaa caaagtcgac ctgcgcgtca agcgcggcac catacacggc
     2341 ctgatcggcc ccaacggctc cggcaagagc accatgatga acgtgctcac cggcatctac
     2401 gtgcccactg caggaggcgt gaccttctcg ggcaactccc tggtgggcct gccgccggcg
     2461 cgcatcgccc tgtcgggcgt ggcgcgcacc ttccagaacg tgcagctttt cggcgagatg
     2521 aacgcgctcg aaaacatcct ggtcggcctg caccatactt tccacaccgg cttcgccggc
     2581 gtggccctgc gcacgccgcg ctggcggcgc gaagaaaacg cggcccgcgc ccgcgcgctg
     2641 gcgctgctgg aattcgtcgg cctgcggaac ctggccctcg aagaagcgcg caacctgccc
     2701 tacggcaagc agcgcctgct cgagatcgcc cgcgccctgg cgctcaatcc cagcctgctg
     2761 ctgctcgacg aaccggccgc cggcctcacc gcccccgaca tcgccgagct gctggccatc
     2821 atccgcaagg tgcgcgacca cggcatcacc ctgatcctga tcgaacacca catggatgtg
     2881 gtcatgagcc tatgcgacac cgtcacggtg ctggatttcg gccagaagat cgccgagggc
     2941 gcgcccgccg cggtgcaggc ggacgagaag gtgatcgccg cctatctcgg cggcaccgtg
     3001 gcggaggccg cctgaatgct gcaagtggac aacctcgtcg ccggctacgg caaggtgcag
     3061 gtgctgcacg gcctttccat gtcggtgccg caaggccagc tcgtgaccct gatcggcagc
     3121 aacggcgccg gcaagaccac gacgctgcgc gccctgtccg gcatgatccg cccggactcc
     3181 ggcagcatca ccctgggcgg ccagcagatc gccgggctgc cgtcgtacac catcgcccgg
     3241 cgcggcctgg cgcattcgcc cgaggcccgg cgggtgttcg ccaccctgtc ggtggccgac
     3301 aacctggtca tgggcgcctt accgcgcctg accggcagcc ggccccgcgg cgacgtggcc
     3361 gccgacctcg atcgcatgtt ccagctgttc cctcgcttgc aggaacgccg cacccaactg
     3421 gccggcaccc tgtcgggcgg cgagcagcag atgctcgcca tggcccgcgc cctgatgctg
     3481 aaccccgacg tgctgctgct ggacgagccc agcatgggcc t
//
LOCUS       DASWPO010000133         5222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_297103, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000133 DASWPO010000000
VERSION     DASWPO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297103"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="VGC69_07505"
     CDS             <1..1474
                     /locus_tag="VGC69_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0295165.1"
                     /translation="LADVQRVSETYLIRDNRTAGRYLPTAQPVRAPLNTRVDLAQVLD
                     SYQGSADFTAVPAFDSTPDNIDQLTQRFTLDLPNGPIQLALLPKPTRGKRVQARLLLQ
                     YGDAAALRGQRVALGVADDLIMRGTPTLTRQAIEDKLDQLQADVSVDGSGTDLVLSLS
                     TTRDNLPALIGLMLDVLRNADYPEAELAEYKRQAIGSIQSAMTEPSALAARALGRQAN
                     PWPADDVRYTPSFEESLAALQALTRDQVLAAHDRFYGAGRLFFSAVGDFDPAATQSAL
                     TQGLKGWRQAPAYARLDNPYRDIPAQHIEINTPDKANAIYLAHAPLRLQDTDPDYPAL
                     SLANFLLGSSETSRLWTRIRDKEGLSYTVRSSLAVSSFEPSATWSIYAIYAPQNKARV
                     ETAMNEELARAVRDGFTADEVRDGIAALLNYRKLSRAQDEVLAGSWLDYMQTNRTFQR
                     SADLDDKIKALTPDMVNAAVRKYLKPQDFSTAEAGDFAKK"
     gene            complement(1648..2796)
                     /gene="dnaJ"
                     /locus_tag="VGC69_07510"
     CDS             complement(1648..2796)
                     /gene="dnaJ"
                     /locus_tag="VGC69_07510"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0295166.1"
                     /translation="MAKRDYYEILGVAKNATDDELKKAYRKLAMKHHPDRNPGSKDAE
                     EKFKEAKEAYEILGDEQKRAAYDRYGHAGVDPNAAGMGAAGAGGMGGGFADAFGDIFG
                     EIFGGAGGGGRRGGGGGPQVYRGGDLKYALEITLEQAAHGFDTEIRVPSWENCDVCHG
                     SGAKPGTTPKTCRTCNGSGAVRMQQGFFSVQQTCPTCHGTGKEISDPCTACEGVGRVR
                     RNKTLQVKIPAGIDDGMRIRSSGNGEPGLNGGPPGDLYVEIHIKQHKIFQRDGDDLHC
                     ELTIPFTTAALGGDLQVPTLGGKAEITIPEGTQSGKTFRLRGKGIRGIRAAYPGDLYC
                     HVLVETPVRLNDEQKAILRQFETSLNDGGDRHSPQSKSWTDRVKEFFS"
     gene            complement(2978..4903)
                     /gene="dnaK"
                     /locus_tag="VGC69_07515"
     CDS             complement(2978..4903)
                     /gene="dnaK"
                     /locus_tag="VGC69_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0295167.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGTQVKIIENAEGTRTTPSIVAYME
                     DGETLVGAPAKRQAVTNPKNTLYAVKRLIGRKFDEKAVQKDIDLMPYAIVKNDNGDAW
                     VQVRDRKLAPPQVSAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKAEKGDRKIAVYDLGGGTFDVSIIEIADVDGEMQFE
                     VLSTNGDTFLGGEDFDQRIIEYIITEFKKESGVDLSKDVLALQRLKEAAEKAKIELSS
                     SQQTEINLPYITADASGPKHLNLKITRAKLEALVEELIERTIEPCRVAIKDAGVKVSD
                     IDDVILVGGMTRMPKVQDKVKEFFGRDPRKDVNPDEAVAAGAAIQGSVLSGDRKDVLL
                     LDVTPLSLGIETLGGVMTKMIQKNTTIPTRFSQTFSTADDNQPAVTIKVFQGEREIAS
                     GNKALGEFNLEGIPPAARGLPQIEVTFDIDANGILHVSAKDKGTGKENKITIKANSGL
                     SEDEIQRMVKDAEANAVEDHRLAELALARNQADGLVHATRKSLTEYGDKLDASEKEAI
                     EAAIKDLEEVLKTGTKEEIDAKVEVLGTASQKLGEKMYADAQAHQQAAAQQAADNAKP
                     ADDNVVDADFKEVKREQ"
     gene            complement(5024..>5222)
                     /gene="grpE"
                     /locus_tag="VGC69_07520"
     CDS             complement(5024..>5222)
                     /gene="grpE"
                     /locus_tag="VGC69_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0295168.1"
                     /translation="GAFERHKLLEIQPPQGEKFDPHKHQAIASVPSEQPANTVLQTLQ
                     KGYLISDRVLRPALVTVVAPK"
BASE COUNT          824 a         1774 c         1623 g         1001 t
ORIGIN      
        1 gctggccgac gtgcagcgcg tgtccgagac ctacctgatc cgcgacaacc gcacggccgg
       61 ccgctacctg ccgacggccc agcccgtgcg cgcgccgctc aacacccgcg tcgacctggc
      121 gcaggtactg gacagctacc agggcagcgc cgatttcacc gccgtgcccg ccttcgattc
      181 gacgcccgac aatatcgacc agctgaccca gcgcttcacg ctggacctgc ccaacggacc
      241 gatccagctc gccctgctgc ccaagcccac gcgcggcaag cgcgtgcagg cgcggctgct
      301 gctgcaatac ggcgatgccg ccgcgctgcg cggccagcgc gtcgcgctcg gcgtggccga
      361 cgacctgatc atgcgaggca cgcccacgct gacgcgccag gccatcgagg acaagctcga
      421 ccagttgcag gccgacgtct cggtcgacgg ctcgggcacc gacctggtgc tcagcctgtc
      481 cactacccgc gacaacctgc ccgcgctcat cggcctgatg ctggacgtgc tgcgcaatgc
      541 cgactatccc gaggcagaac tggccgaata caagcgccag gccatcggct ccatccagag
      601 cgcgatgacc gagccttccg ccctggcggc gcgcgccctg ggccgccagg ccaacccctg
      661 gccggccgac gacgtgcgct acacgcccag cttcgaggag tccctggccg cgctgcaagc
      721 gctgacccgc gaccaggtgc tcgcggcgca cgaccgcttc tacggcgccg gccgcctctt
      781 tttctcggcc gtgggcgact tcgacccggc cgccacgcaa tccgccctga cccagggcct
      841 caagggctgg cggcaagccc cggcctatgc gcgcctggac aatccgtacc gcgacattcc
      901 cgcgcagcac atcgagatca acacgcccga caaggccaac gccatctacc tggcgcacgc
      961 gcccctgcgc ctgcaagaca ccgatcccga ctacccggcc ctgtcgctgg ccaatttcct
     1021 gctgggctcg tccgaaacct cgcgcctatg gacccgtatt cgtgacaagg aaggcctgtc
     1081 ctacaccgtg cgcagcagcc tggccgtctc gtccttcgag ccctcggcca cctggtcgat
     1141 ctatgccatc tatgccccgc agaacaaggc gcgcgtcgag acggccatga acgaagaact
     1201 ggcccgcgcc gtccgcgacg gcttcaccgc cgacgaagtc cgcgacggca tcgccgccct
     1261 gctcaactac cgcaagctgt cgcgcgcgca ggacgaggta ctggccggct cctggctgga
     1321 ctacatgcag accaaccgca ccttccagcg ctcggccgac ctggacgaca agatcaaggc
     1381 gctgacgccc gacatggtga acgccgccgt gcgcaaatac ctgaagcccc aggacttcag
     1441 cacggccgag gcgggagact tcgccaagaa ataagccctt cgatcgaagg gcttggtcag
     1501 tggcctctgt cggaagcgct tgagatctgt gagccgagga ccccggaggg atccgaagcg
     1561 caacccccgg tgggggcgag catcggaatt ccgcagggca gactcggcgg ctcaagaacc
     1621 cagatagggc tcaagaaccc aaccggatca ggagaaaaac tccttcaccc ggtccgtcca
     1681 cgacttgctc tgcggcgaat ggcgatcgcc gccatcgttg agcgaagtct cgaactgccg
     1741 caggatggcc ttctgctcgt cgttcaggcg caccggcgtc tccaccagca catggcagta
     1801 caggtcgccc gggtaggccg cgcgtatccc gcgtatgccc ttgccgcgca ggcggaaggt
     1861 cttgcccgac tgggtgcctt cggggatggt gatctcggcc ttgccgccca gcgtgggcac
     1921 ctgcaggtcg ccgcccagcg ccgccgtggt gaacgggatg gtcagctcgc aatgcaggtc
     1981 gtcgccgtcg cgctggaaga tcttgtgctg cttgatgtgg atttccacat acaggtcgcc
     2041 cggcggcccg ccgttgaggc ccggctcgcc attgcccgaa gagcggatgc gcatgccgtc
     2101 gtcgatgccg gcggggatct tcacctgcag ggtcttgttg cggcgcacgc ggcccacgcc
     2161 ttcgcaagcc gtgcagggat cggagatctc cttgccggtg ccgtggcagg tgggacaggt
     2221 ctgctgcacg ctgaagaagc cctgctgcat gcgcaccgcg cccgaaccgt tgcaggtgcg
     2281 gcaagtcttg ggcgtggtgc cgggcttggc gccggaacca tggcagacgt cgcagttttc
     2341 ccagctgggc acgcggattt cggtatcgaa gccgtgggcg gcctgctcga gcgtgatctc
     2401 cagcgcatac ttcaggtcgc cgccgcggta gacctgcgga ccgccgccgc cgccacgccg
     2461 gccgccgcca cctgcgccgc caaaaatctc gccgaagatg tcgccgaagg catcggcaaa
     2521 accgccgccc atgccgccag cccccgccgc gcccatgccc gcggcattgg ggtcgacgcc
     2581 ggcgtggccg tagcgatcgt aggccgcgcg cttctgttcg tcgcccagga tctcgtaggc
     2641 ctccttggcc tccttgaatt tttcctcggc atccttgctg cccggattgc ggtccgggtg
     2701 gtgcttcatg gccagcttgc ggtaggcctt cttgagctcg tcgtccgtgg cgttcttggc
     2761 cacgccgaga atttcgtagt aatcgcgttt tgccatggtg gaaatgagtg ctttagaaac
     2821 caggctacag gaaacgcccc cacgccgcgc cttcggctag ctgcccccga gggggacttt
     2881 tgccttgggg cggcccggcg gcaaaaatac gctcgcccgg cagcctgaaa cggccccggg
     2941 cggcgctgct gcagtgggca aaatcgccca ccctacatca ctgctcgcgc ttgacttcct
     3001 tgaagtcggc gtcgaccacg ttgtcgtcgg ccggcttcgc gttgtcagcc gcctgctggg
     3061 ccgccgcctg ctgatgagcc tgggcgtcgg catacatctt ctcgcccagc ttctgagaag
     3121 ccgtgcccag cacctcgacc ttggcatcga tctcttcctt ggtgcccgtc ttcagcacct
     3181 cttcgagatc cttgatggcc gcctcgatgg cttccttctc ggacgcgtcc agcttgtcgc
     3241 catactcggt caacgacttg cgggtggcgt gcaccaggcc gtcggcctgg ttgcgggcca
     3301 gggccagctc ggccaggcgg tggtcttcga ccgcgttggc ttcggcgtcc ttgaccatgc
     3361 gctggatctc gtcttccgac aaacccgaat tggccttgat ggtgatcttg ttttccttgc
     3421 cggtgccctt gtccttggcg gacacgtgca ggatgccgtt ggcgtcgatg tcgaaggtga
     3481 cctcgatctg cggcaggccg cgcgccgccg ggggaatgcc ctcgaggttg aactcgccca
     3541 gggccttgtt gcccgaggcg atctcacgct cgccctggaa caccttgatc gtcaccgccg
     3601 gctggttgtc gtcggcggtc gagaacgttt gcgagaaacg cgtcgggatc gtggtgttct
     3661 tctggatcat cttggtcatc acgccgccca gggtctcgat gcccagcgac agcggggtga
     3721 cgtcgagcag cagcacgtcc ttgcggtcgc ccgacagcac ggagccctgg atggcggcgc
     3781 cggcggcgac cgcttcatcg gggttcacgt ccttgcgcgg atcgcggccg aagaactcct
     3841 tgaccttgtc ctgcaccttg ggcatgcggg tcatgccgcc gaccaggatc acgtcgtcga
     3901 tgtccgaaac cttcacgccg gcgtccttga tggccacgcg gcaaggctcg atggtgcgct
     3961 cgatcagctc ttcgaccagg gcttccagct tggcgcgcgt gatcttcagg ttcaagtgct
     4021 tggggcccga ggcgtcggcc gtgatgtacg gcaggttgat ctcggtctgc tggctcgagg
     4081 acagctcgat cttggccttt tcggcggctt ccttcaggcg ctgcagggcc agcacgtcct
     4141 tggacaggtc gacgcccgat tccttcttga actcggtgat gatgtattcg atgatgcgct
     4201 ggtcgaagtc ttcgccgccc aggaaggtgt cgccgttggt cgacagcact tcgaactgca
     4261 tctcgccgtc gacgtcggcg atttcgatga tggacacgtc gaaggtgccg ccgcccaggt
     4321 catagacggc gatcttgcgg tcgcccttct cggccttgtc caggccgaag gccagcgcgg
     4381 ccgcggtggg ctcgttgatg atgcgcttga cttccaggcc ggcgatgcgg ccggcgtcct
     4441 tggtggcctg gcgctggctg tcgttgaagt aggccgggac ggtaatgacc gcttcggtca
     4501 cttcttcgcc cagatagtct tcagccgtct tcttcatctt gcgcagcact tcggccgaca
     4561 cttgcggcgg cgccagcttg cggtcgcgca cctgcaccca tgcgtcgcca ttgtcattct
     4621 tgacgatggc atagggcatc aggtcgatgt ctttctgtac cgctttttcg tcgaacttgc
     4681 gtccgatcag gcgcttgacg gcgtacaggg tgttcttggg gttggtgact gcctggcgct
     4741 tggccggcgc gcccaccagc gtttcaccgt cttccatata ggcgacgatg gacggcgtgg
     4801 tgcgggtgcc ttcggcgttt tcgatgattt tgacctgcgt accttccatt acagccacgc
     4861 agctgttggt ggttccgaga tcgataccta tgattttgcc cataatcctt attcctgaaa
     4921 aaatcagaaa actctttgat gactaaatag gttgcaagca ggttttttca atacccgcga
     4981 tagccgcaat ttatagtcgc ccgcggcagg gcgcacaaac ggcttatttc ggcgccacga
     5041 cggttaccaa agctggccgc aaaacccggt cggaaatcag gtagcctttc tgcagcgtct
     5101 gcaaaacggt attggccggc tgctcggacg gcaccgacgc aatggcctgg tgcttgtgcg
     5161 gatcgaactt ttcaccttgc ggcggctgga tttccagcaa tttgtgccgt tcgaacgcac
     5221 cg
//
LOCUS       DASWPO010000134         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_566150, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000134 DASWPO010000000
VERSION     DASWPO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566150"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VGC69_07525"
     CDS             <1..908
                     /locus_tag="VGC69_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0295169.1"
                     /translation="LNAAAIPRDLLEAELFGHERGAFTGANTLRRGRFEEAHGGTLFL
                     DEIGDMPIELQTRLLRVLAEGSFYRVGGSQPVRVDVRIVAATHQPLEQRVEQGLFRED
                     LFHRLNVIRLRLPPLRERIEDIPALANHFLTNSARSLGVPVKRLTPEAIAVLTRFDFP
                     GNVRQLENFCHWLTVMAPGQTIDRNDLPPEIRAIEQGGTAPGSLFRPVAAPSDTAPAG
                     IVAPSASEGGAGSWQEALLRDAQRRLERGEPAVMATLTRQFEKILLQSALDASRGRRV
                     EAASRLGIGRNTITRKLRELGIEDE"
     gene            973..2190
                     /locus_tag="VGC69_07530"
     CDS             973..2190
                     /locus_tag="VGC69_07530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0295170.1"
                     /translation="MHLDIETVQVVTLFVTFVSGWLLLFAWLPNRRLTSLAMWGAGCI
                     TASSGEFLMIERESLSSFLFVDLAGALILLGYGLIWSGLRQFAGNPVLGKLVVGPILI
                     WLVACRLGWLSPNSDVHVIVLLLSLAAYLIAGSYEAWRSDPEVVSRWPAVVFLLAKAI
                     AYMAAAVLQNRLPPPLGHATEVPYWIVFVYFGFLLIVCTLIFLAISMAKERIELDYRR
                     ASQIDSLTGIANRRAFFNVGNRLLQRTLAEKKTVAILLMDLDAFKRVNDNYGHQIGDR
                     LLRNFSEAVRATLRNADYFGRLGGQEFACLLPGVAEAQAHLVAERIRVGCEDQRVTRG
                     GARYIGTTVSIGIVVAHAPGYSLDILLADANLALYQAKAKGRNRVEQVKASGAVPDEA
                     DESRYVRDGSLQH"
     gene            complement(2212..3150)
                     /locus_tag="VGC69_07535"
     CDS             complement(2212..3150)
                     /locus_tag="VGC69_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295171.1"
                     /translation="MSQQPITPELLSMVDAIARHGSFARAARELGKVPSAVTYAVRKL
                     EDGLDVLLFDRSGHRAQLTQAGETLLHDGRHVLQSLDDLAYRVKRVATGWEVELRIAV
                     SAVLPWRPFYALIEAFRQVGAGTTLRFSAEVLSGTWDALASGRADLVIGADAAGAPAG
                     AFRSQAMGRTQFGFCVAPHHPLAALSARRALTAEDIAAHCAVVVADTSRDLPARTRGL
                     LTQQSMIIMPTMQDKVEAQVRGLGCGYLPLTLAAAHIARGQLVICATEEGIALDENVV
                     YAWQARQPGAGLAWWLEKLKSPKLCEHLLGLADVMP"
     gene            3355..4065
                     /locus_tag="VGC69_07540"
     CDS             3355..4065
                     /locus_tag="VGC69_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0295172.1"
                     /translation="MLTLRPANQRGHANHGWLDSYHTFSFANYFDPGHMGFGPLRVIN
                     DDRIAPGRGFGTHGHRDMEIITYVLDGAIAHKDSMGSGSTIEPGNVQRMSAGRGVLHS
                     EFNPQADKETHMLQIWIEPNVAGITPEYEEKTFSNDEKRGRLRQLVSPDGADGSMVIH
                     QDVRLYAGLFDGAEADTLPLARGRHAWVHVARGSLTVNGQKLNEGDALAISDDAAVRL
                     GEGADAEVLVFDLPSNEK"
     gene            4134..>4869
                     /locus_tag="VGC69_07545"
     CDS             4134..>4869
                     /locus_tag="VGC69_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0295173.1"
                     /translation="MSELSPESAVEAIVIPRTSDIGNFEVRRALPSMQKRTVGPFVFL
                     DEMGPAVIPAGIGLDVRPHPHIGLETVTYLFDGSILHRDGLGSIQLIEPGEVNWMTAG
                     RGIVHSERSTPEARRVDQRVFGLQMWVALPHDKEEMDPSFIHYGLDAQPVVEGEGVWA
                     RVVAGSLFGRTSGVKTQSPLFYGDIKLEPGASVVLSAEHVERAAYLAEGTVQVEGIDH
                     PPGRLLAFAPGRPVTLTAVAGPARFAV"
BASE COUNT          800 a         1703 c         1581 g          785 t
ORIGIN      
        1 cgctcaatgc cgccgcgatc ccgcgcgacc tgctcgaggc cgaactcttc ggccacgaac
       61 gcggcgcctt caccggcgcc aacacgctgc gccgcggccg cttcgaggag gcccatggcg
      121 gcacgctgtt cctcgacgaa atcggcgaca tgcccatcga gctgcagacg cgcctgttgc
      181 gcgtgctggc cgaaggcagc ttctaccgcg tgggcggctc gcaaccggtg cgcgtggacg
      241 tgcgcatcgt cgccgccacc caccagccgc tggagcaacg cgtggagcag ggcctgttcc
      301 gcgaagacct gttccaccgc ctgaacgtga tccgcctgcg cctgccgccg ttgcgcgagc
      361 gcatcgagga cattccggcg ctggccaacc atttcctgac caacagcgcc cgttcgctgg
      421 gggtgcccgt caagcgcctg acgcccgaag ccatcgccgt gctcacgcgc ttcgacttcc
      481 ccggcaatgt gcgccagctg gaaaacttct gccattggct caccgtcatg gcgcccggcc
      541 agaccatcga ccgcaacgac ctgccgcccg aaatccgcgc gatcgagcag ggcggcacgg
      601 cgccgggcag cctcttccgg cccgtggcgg cgccgagcga caccgcgccg gccggcatcg
      661 tcgcgccctc ggccagcgag ggcggcgccg gctcctggca ggaagccctg ttgcgcgacg
      721 cgcagcggcg gctggagcgc ggcgagccgg ccgtcatggc cacgctcacg cgccagttcg
      781 agaaaatcct gctgcagagc gcgctcgacg ccagccgcgg ccgccgggtc gaggccgcct
      841 cgcggctggg catcggccgc aataccatta cccgcaagct gcgagaactc ggtatcgaag
      901 acgaataagt cccgggcgga gggtttgcgc gttaccattg ttacttttgt cacgagtagc
      961 ggataggagc gcatgcatct agacatcgag accgtccagg tcgtcaccct ctttgtcacc
     1021 tttgtttcag gctggctgct gctgttcgcc tggctgccga accggcgcct cacttcgctc
     1081 gcgatgtggg gggctggctg catcaccgcg tcgagcggcg agttcctcat gatcgagcgc
     1141 gagagcttgt ccagctttct gttcgtcgat cttgccgggg cgctgatcct gctgggttac
     1201 ggcctgatct ggagcggcct gcgccagttc gcgggcaatc cggtgctggg caagctggtg
     1261 gtggggccga tattgatctg gctcgtggcc tgccgcctgg ggtggctgag cccgaactcc
     1321 gacgtccacg tcattgtctt gctgctgagc ctggcggcct acctcattgc cggcagctac
     1381 gaggcctggc gcagcgatcc cgaagtggtc tcgcgctggc cggcggtggt ctttctgctg
     1441 gccaaggcca ttgcctacat ggccgccgcc gtgctgcaga acaggctgcc gccgccgctc
     1501 gggcatgcca ccgaggtgcc ctattggatc gtgttcgtct acttcggctt cctgctgatc
     1561 gtctgtaccc tgatcttcct ggccatcagc atggccaagg aacgcattga gctcgactat
     1621 cgccgcgcgt cgcaaatcga ctcgctgacc ggcattgcca atcgccgcgc ttttttcaac
     1681 gtcggcaacc gcttgctcca gcgcacgctg gccgagaaga aaaccgtggc catcctgctg
     1741 atggacctgg acgccttcaa gcgcgtcaat gacaactacg gccaccagat cggcgaccgg
     1801 ctattgcgca atttcagcga agcggtgcgc gccacgctgc gcaacgccga ctatttcggc
     1861 cggctgggcg ggcaggaatt cgcctgcctg ctgcctggcg tggccgaagc ccaggcccac
     1921 ctggtcgccg agcgcatccg cgtgggatgc gaagaccagc gcgtcacgcg cggcggagcc
     1981 cgttatatcg gcaccacggt cagcatcggc atcgtggtgg cccatgcgcc cggctactcc
     2041 ctggacatcc tgctggccga tgccaatctt gccctgtacc aggccaaggc caaggggcgc
     2101 aaccgcgtcg aacaggtcaa ggcctccggc gcggttccgg acgaagcgga tgaatcgcgc
     2161 tatgtgcgcg acggcagcct gcagcactag ccgtgcaagg gccgcgtcgg cttacggcat
     2221 gacgtccgcg agccccagca ggtgctcgca gagcttgggc gacttgagct tttccagcca
     2281 ccaggccagg cccgcgccgg gctgcctggc ctgccaggca tacaccacgt tctcgtccag
     2341 cgcgatgcct tcttcggtgg cgcagatcac cagctgcccc cgggcgatgt gcgcggcggc
     2401 cagcgtcagc ggcaggtagc cgcaacccag cccgcgcacc tgcgcctcga ccttgtcctg
     2461 catggtcggc atgatgatca tggattgctg cgtgagcagc ccgcgcgtgc gggccggcag
     2521 gtcgcgcgag gtatcggcca ccaccacggc gcaatgggcc gcgatgtcct cggccgtcag
     2581 ggcccgccgg gccgacaggg cggccagcgg gtggtgcggc gccacgcaga aaccgaactg
     2641 cgtgcgcccc atggcctgcg aacggaaggc gccggccggc gcgcccgccg cgtcggcccc
     2701 gatgaccagg tcggcccggc ctgaggccag ggcatcccag gtgccgctca gcacctcggc
     2761 ggaaaagcgc agcgtggtgc ccgcgcccac ctggcggaac gcctcgatca gggcatagaa
     2821 gggccgccag ggcagcaccg cgctgacggc gatgcgcagc tccacctccc agcccgtggc
     2881 cacgcgcttg acgcgatagg ccaggtcgtc gagcgattgc agcacgtggc ggccgtcgtg
     2941 cagcagggtc tcgccggcct gggtgagctg ggcccggtgg ccgctgcggt cgaacagcag
     3001 cacgtccagc ccgtcttcca gcttgcgcac ggcataggtg acggccgagg gcaccttgcc
     3061 cagctcccgc gccgcccggg caaagctgcc gtggcgggcg atggcgtcga ccatggaaag
     3121 cagttcgggc gtgatgggct gctgcgacat ggaaatctcc ggtggaaacg ggccccgcgg
     3181 ggggcatggc aggccgcgaa agatcattct aattatttga atgatctcat caaatccgtt
     3241 cgccctcaag cctgggccaa aagcccacaa tacatctcat caggccagca caaagagcga
     3301 ttcaaaccag aggctctcgt gcaaaaccgt tcaccgatat tcaaggagct cactatgctt
     3361 actctgcgcc cagccaatca gcgcggccat gccaaccacg gttggctcga cagctatcac
     3421 accttttcgt tcgccaacta cttcgacccc ggccacatgg gttttggccc gctgcgcgtg
     3481 atcaatgacg accgtatcgc cccgggacgc ggcttcggca cgcacggcca tcgcgacatg
     3541 gaaatcatca cctatgtgct cgacggcgcc atcgcccaca aggacagcat gggcagcggc
     3601 tcgaccatcg agccgggcaa tgtccagcgc atgagcgccg gccgcggcgt gctgcattcg
     3661 gagttcaacc cgcaggccga caaggaaacg cacatgctgc agatctggat cgaacccaat
     3721 gtggccggca ttacgcccga atacgaagag aagacattct ccaatgacga aaagcgcggc
     3781 cggctgcgcc agctggtttc gcccgacggc gccgacggct cgatggtcat ccaccaggat
     3841 gtccgcctgt atgccggcct gttcgacggc gccgaggccg acacgctgcc cctggcccgc
     3901 ggccgccacg cctgggtcca cgtggcccgc ggcagcctga cggtcaacgg ccagaagctg
     3961 aacgagggcg acgccctggc catcagcgac gacgccgcgg tgcggctggg cgagggcgcg
     4021 gacgccgaag tgctggtgtt cgacctgcct tcgaacgaga agtaaatcgc agcgacgcga
     4081 tgggtatcat gtgggtagca tgacgcccat cgctgcccat ccaggagaca ggcatgtccg
     4141 aactcagtcc cgaaagcgcc gtcgaggcca tcgtcatccc gcgcaccagc gacatcggca
     4201 attttgaagt gcggcgcgcg ctgccttcca tgcagaaacg cacggtgggg ccattcgtct
     4261 tcctcgacga aatgggcccg gcggtgattc ccgccggcat cggcctggac gtgcggccgc
     4321 acccgcacat cggcctggaa accgtcacct atctgttcga cggatcgatc ctgcaccgcg
     4381 acggcctggg cagcatccag ctcatcgagc ccggcgaggt caactggatg accgccggcc
     4441 gcggcatcgt gcactccgaa cgctccacgc ccgaagcccg ccgggtcgac cagcgcgtct
     4501 tcggcctgca gatgtgggtg gccctgccgc acgacaagga agagatggac cccagcttta
     4561 tccactatgg cctggatgcc cagcctgtgg tcgaaggcga gggcgtctgg gcccgcgtgg
     4621 tggccggctc gctttttggc cggacctccg gggtcaagac ccagtcgccg ctcttctacg
     4681 gcgacatcaa gctcgagccg ggcgcctcgg tggtgctgtc ggccgagcac gtcgaacgcg
     4741 ccgcctacct ggccgaaggc acggtccagg tggaggggat cgatcacccg cccggccgcc
     4801 tgctggcctt tgcgcccggc cgacccgtca cgcttaccgc cgtcgccggc ccggcgcgtt
     4861 ttgccgtgg
//
LOCUS       DASWPO010000135        10354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1637578, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000135 DASWPO010000000
VERSION     DASWPO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637578"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /gene="lpxA"
                     /locus_tag="VGC69_07550"
     CDS             complement(<1..727)
                     /gene="lpxA"
                     /locus_tag="VGC69_07550"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY0295174.1"
                     /translation="MSGNIHSTALVDPAAKLDASVSVGPYSIIGPDVTIGAGTEIGAH
                     CVIDGITTIGRDNRFYRYCSIGGMPQDKKYAGEATELIIGDRNTVREFTTFNTGTVQD
                     GGKTTVGSDNWIMAYVHIAHDCHIGSNTILANSAQLAGHIEVHDWAIVGGMTGVHQFC
                     KIGAHCMVGGGTTLVQDIPPFMLASGSDGPCRPAGINVEGLKRRGFSAVTISALREAY
                     KSLYRRGLTLDEASLQIRAQQQAE"
     gene            complement(727..1182)
                     /gene="fabZ"
                     /locus_tag="VGC69_07555"
     CDS             complement(727..1182)
                     /gene="fabZ"
                     /locus_tag="VGC69_07555"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY0295175.1"
                     /translation="MELDIKGIMERLPHRYPMLLVDRVLEMVPGKSIVAIKNVSINEP
                     FFTGHFPHLPVMPGVLIIEAMAQAAAIFSFTNGDGGTFADCSKTAYYLVGVDGARFRR
                     PVVPGDQLRLEVEAERLSRSICKYKGRALVDGQEVAEASLMCAIRSLES"
     gene            complement(1258..2373)
                     /gene="lpxD"
                     /locus_tag="VGC69_07560"
     CDS             complement(1258..2373)
                     /gene="lpxD"
                     /locus_tag="VGC69_07560"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY0295176.1"
                     /translation="MPVLLDLAHAPSLDKLLAGANTQGLAWRIETLGQAGQALPQVCG
                     IGTLSGAGPNEVSFLTNKRYQSQLLDTKAAAVIVPEQAVELLKPYAPLPFAVVVSDQP
                     YLLYARLAQWFDLARRPLEPSGVHPTAVVAADAHLEEGVRVGPHCVVESGARIGRGTV
                     LGPACIIGQGSQLGPDCLLHARVTIYHGVHVGARAILHSGVVLGADGFGFAPDATLAK
                     GAWGKIPQLGGVRVGDDVEIGANTTVDRGALEDTLIADGVKLDNLIMIAHNVRVGAHT
                     AIAGCVAVAGSAVIGERCIIGGHSAIAGHITLADDVVVSGGTGIFSSIDKPGQYTAVY
                     PYSEHAQWQRNAAVVPHLADLRKRVRALEKKNADDAA"
     gene            complement(2406..2945)
                     /locus_tag="VGC69_07565"
     CDS             complement(2406..2945)
                     /locus_tag="VGC69_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY0295177.1"
                     /translation="MTQSRIVRRSAMALAAALLMGSAALGTAQAQSATAPSQGTKIGF
                     VNTERILRESVPAKAAQSKIEAEFKSRDADLTRLSNTLRTKADQFEKDSPVLSDSDRT
                     TRQRELSNLDMDLQRKRREFQEDFNRRRNEEFSSIVTKANDAIKRIAEQENYDLIVQD
                     AVTVNPRIDITDKVIQSLH"
     gene            complement(3068..5410)
                     /gene="bamA"
                     /locus_tag="VGC69_07570"
     CDS             complement(3068..5410)
                     /gene="bamA"
                     /locus_tag="VGC69_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY0295178.1"
                     /translation="MSFRRMFQRVLQSSLRYLLTSVILFAPTWALAFEPFVVKDIRVE
                     GLQRVDAGTVFGFMPVKVGQRFTDDQASEAVRRLYGSNFFSDVQIRTEGDVVIVAVQE
                     RPTVASITFSGMREFESKNIVSALAKVGFAEGRIFDQSMLDQAENELKQQYLSKGKYS
                     TEVTATVTPLPRNRVGIAFDIFEGEVAKIREIHFVGNKTFSESTLLDEVDMTTPGWLT
                     WYTNTDKYSREKLEGDIDKIRSFYLNRGYLEYSNDPPQVTISPDRQSIYITVTIHEGE
                     PYKVRDVKLAGNLLGLDDEIKKMVQIKPGTVFNAEQANNSAKAITNYLGELGYAFANV
                     NPNPQLDREKHEADLTFYVDPSRRVYVRRIQMGGNTRTRDEVIRREMRQQEAAWYDAG
                     EIRTSRDRIDRLGYFTDVDIKTDPVPNSPDQVDLNVNVKEKPTGIINLGIGYGSADKA
                     IFSAGISQDNIFGSGTNMSFNVGTSDVQTSAVLEQTDPYFTKDGISRTVSLYYRLTRP
                     WVGVTGNYAVRTIGAGLSFGVPITETDRIFMGANLERNDVSLYSDSPQAYDDFVNAYG
                     QKTNTVILNAGWSKDTRDSALAPTRGSYTKLRADVGTMDLKYTMFSAQQQYFIPLNRS
                     YTLAFNGLANYGHSYGGNIFPVIKNVYGGGLGSVRGFESSSLGPRDTLTGDYIGGQRM
                     LAANIQLYLPFPGASHDRTLRWFLFLDGGQVSTTGSLTCSSGSATTQVVDPCGWRYSA
                     GVGLSWQSPLGPLQLSFGHALNAKQGDNKEAFQFQVGTAF"
     gene            complement(5465..6802)
                     /gene="rseP"
                     /locus_tag="VGC69_07575"
     CDS             complement(5465..6802)
                     /gene="rseP"
                     /locus_tag="VGC69_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY0295179.1"
                     /translation="MLFTLVAFAVALGILITFHELGHYGVARLCGVRVLRFSIGFGRV
                     LLRRTDRHGTEWAVSAIPLGGYVKMQDDPPAGASKAEAARAFNKQSVWRRMAIVAAGP
                     VFNLVLAVVFYAALDMAGAQVPAPILGQPTAGSPAEQAGFRQGDRIIAVDGKAVESWD
                     DARWDLIDVLSSGGKSVLDVQPAEGGALQRTLVLASGNHMDPEQGDPLAWAGFHLAQP
                     RPVVRQVIAGSAGEAAGLQAGDLITAAGELAGQLDASALVGYIQQHAGQPLPMTVMRG
                     GVPISLHVVPHAEQVDGRQVGRIGVQLGGDLPMVTVRYGVFDSVGRGARRTWDTAWLT
                     LRMMGRMVVGDVSWRNISGPVTIADYAGQTARIGLAAYVAFLALISISLGVLNLLPIP
                     MLDGGHLLYYLLEIVRGRPVPDRWMEIGQRAGMGLLACLMGLALFNDFARLFT"
     gene            complement(6860..8077)
                     /locus_tag="VGC69_07580"
     CDS             complement(6860..8077)
                     /locus_tag="VGC69_07580"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY0295180.1"
                     /translation="MSQSGRPQRVVVLGSTGSVGRSTLDVIARHPERLQVHALSAHSR
                     MESLAEQAAACRAPVVLVPDDAARERFTAAWRGAALPEIRVGAQALAETAAEAGCDMV
                     MAAIVGAAGLPSALAAARAGKRVLLANKEALVAAGTLFMQAVRDSGAELLPIDSEHNA
                     IFQCLPHHGASGGRAGAPAEPAAGVRRLLLTASGGPFRRTALEDLQDVTPEQACAHPN
                     WSMGRKISVDSATMLNKGLEVIEAHWLFAMPPERIDVVVHPQSVVHSMVEYEDGSVLA
                     QLGQPDMRTPISYGLGFPERLASGVGGLDLARLGRLDFEAPDFTRFPCLRLSFEALKA
                     GQDACIALNAANEVAVQAFLDGRLPYPWIARVIEATLEWQAGQPSATLGSLQDVLAQD
                     AAARAYAGDLGLA"
     gene            complement(8080..8937)
                     /locus_tag="VGC69_07585"
     CDS             complement(8080..8937)
                     /locus_tag="VGC69_07585"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY0295181.1"
                     /translation="MLGQRIITAVVLLIVLAAAIAAPQPVWMLALLSLAAACACWEWL
                     RLTLPARVPAAVPRVIGIVVFAFLLALSWVWSAPTGGVLPEELLAFGVLVVIVSLIWL
                     IPAVIAVVRGRSDAPPASVGWSAFAVLATVTAWAVLTVMYLAFGPLYVLTLLALVWAA
                     DICAYFGGRKFGRRKLAPRVSPGKTVEGALSGLVGAVVVTLVGAWWPDTFGYMLVDRW
                     SIWGAILIAAFLGAVSIMGDLFESLLKRRAGVKDSSGLLPGHGGVYDRLDAILPVVPI
                     ALLVSGALF"
     gene            complement(8975..9733)
                     /gene="uppS"
                     /locus_tag="VGC69_07590"
     CDS             complement(8975..9733)
                     /gene="uppS"
                     /locus_tag="VGC69_07590"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY0295182.1"
                     /translation="MVISSTQVVPEVSGVPQHIAIIMDGNGRWATRRHLPRTAGHVKG
                     VQAVRRAVESCGRLGVRYLTLFAFSSENWRRPADEVSLLMRLFVRSLEREVGKLEKQG
                     VRLRVVGDLSPFEPRLQDLIHQAEARTECNDKLCLSIAANYGGRWDVLQAMRAMLRAN
                     PGLAGHPEQIEETALAEHLSMSWAPEPDLFIRTGGEQRISNFMIWQLAYTELYFTDAY
                     WPDFDGADLEEAIAWYSGRERRFGRTSAQVLATR"
     gene            complement(9773..10333)
                     /gene="frr"
                     /locus_tag="VGC69_07595"
     CDS             complement(9773..10333)
                     /gene="frr"
                     /locus_tag="VGC69_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY0295183.1"
                     /translation="MSVADIRQSADSRMTKSLDTLKANLGKLRTGRAHPSILDHVHVE
                     YYGSPVPVGQVANLTLVDARTVSVQPYEKSMLAPIEKAIRESDLGLNPMALGDTIRVP
                     MPALTEERRRDLTKVARSEGEDAKVAVRNLRREANDGLKKLVKDKVISEDEERRAQDD
                     VQKLTDRYVADIDKLIAQKEAEIMTV"
BASE COUNT         1735 a         3306 c         3574 g         1739 t
ORIGIN      
        1 gctcggcctg ttgctgggcg cggatctgca ggctcgcctc gtcgagcgtg agcccgcggc
       61 ggtagagcga cttgtaggcc tcgcgcaagg ccgagatggt gaccgcgctg aagccgcgcc
      121 gcttgaggcc ctcgacgttg atgccggccg gccggcacgg gccgtcgctg cccgaggcca
      181 gcatgaaggg cgggatgtcc tgtaccagcg tcgtgccgcc gcccaccatg cagtgcgcgc
      241 cgatcttgca gaactggtgc acgcccgtca tgccgccgac gatggcccaa tcgtgcactt
      301 cgatgtggcc ggccaactgc gccgagttgg ccaggatggt gttgctgccg atgtggcagt
      361 cgtgcgcaat gtgcacgtac gccatgatcc agttgtcgct gccgaccgtg gtcttgccgc
      421 cgtcctgcac cgtgccggtg ttgaaggtgg tgaactcgcg gacggtgttg cggtcgccga
      481 tgatgagctc ggtggcctcg cccgcatact tcttgtcctg cggcatgccg ccgatggagc
      541 aatagcggta gaagcggttg tcgcggccga tcgtggtgat gccgtcgatc acgcagtgcg
      601 cgccgatctc ggtgccggcg ccgatggtga catcgggccc gatgatgctg taggggccca
      661 cgctcaccga agcgtcgagc ttggcggcgg ggtcgaccag ggcggtagaa tggatgtttc
      721 cggacattag ctttccaggc tgcgaatggc gcacatcagc gaggcttcgg ccacttcctg
      781 gccgtcgacc agggcgcgcc ccttgtattt gcagatactg cggctcaggc gttcggcctc
      841 gacctccagg cgcagctggt cgccgggcac gacgggccgg cgaaagcgcg caccgtcgac
      901 gcccaccagg tagtaggccg tcttgctgca atcggcgaat gtcccgccgt cgccgttggt
      961 aaacgagaaa atggccgcgg cctgcgccat ggcttcgatg atgagcacgc ccggcatcac
     1021 gggcaaatgc gggaagtggc cggtgaagaa cggctcgttg atcgagacgt ttttgatggc
     1081 cacgatggac ttgcccggca ccatttccag aacgcggtcg acgagcagca tcggataccg
     1141 atgcggcaac cgctccataa tccccttgat gtcgagttcc atgttgagag ttttcctgaa
     1201 aacaagttcg aaattttatt gcgccgccgc aatgcggcca cgctgcctgc gccgcggtca
     1261 cgccgcgtcg tcggcgtttt tcttttccag cgcccgcacg cgcttgcgca aatccgccag
     1321 gtggggaacc accgcagcgt ttcgctgcca ttgggcatgt tcggagtaag gatacacggc
     1381 cgtgtattgg ccaggcttgt cgatgctgga gaaaatgccc gtcccgcccg acaccaccac
     1441 gtcgtcggcg agcgtgatgt ggcccgcaat cgccgaatgc ccgccgataa tgcagcgctc
     1501 gccgatgacc gccgagccgg ccaccgccac gcagcccgcg atggcggtat gcgccccgac
     1561 gcgcacgttg tgggcgatca tgatcaggtt gtcgagcttg acgccgtcgg caatcagggt
     1621 gtcctcgagg gcgccgcggt caacggtggt attggcgccg atttcgacat cgtcgcccac
     1681 cctgacgccg cccagttggg ggatcttgcc ccaggccccc ttggccaggg tagcgtccgg
     1741 cgcgaagccg aagccatcgg cgcccagcac cacgccgcta tggagaatgg cgcgggcgcc
     1801 cacgtgcacg ccgtgataaa tcgtaacgcg ggcatgcagc aggcagtccg gccccaattg
     1861 cgagccctgg ccgatgatgc aagccggccc cagcacggtg ccgcggccga tgcgcgcgcc
     1921 cgattcgacc acgcaatgcg ggccgacgcg cacgccttct tcgagatggg catcagccgc
     1981 caccacggcg gtgggatgca cgcccgacgg ctcgagcggc cggcgcgcaa ggtcgaacca
     2041 ctgcgccagg cgggcgtaga gcagataggg ctggtcgctg accaccaccg caaagggcag
     2101 cggcgcatag ggcttgagca gctcgacggc ctgttccggc acgatgacgg cggccgcctt
     2161 ggtgtccagc agctgcgact ggtagcgttt attggtgaga aagctgactt cgttgggccc
     2221 ggcgcccgac aaggtgccga tgccgcaaac ctggggcaag gcctgcccgg cctggcccag
     2281 cgtttcgatc cgccatgcca ggccctgcgt attggcgccc gccaggagtt tgtcgaggct
     2341 gggcgcgtgg gcgaggtcca acaaaaccgg catggcaaaa aaaccgaaat acgaccgcta
     2401 tgcgatcagt gcaggctttg aatcaccttg tcggtgatgt cgatgcgcgg attgaccgtg
     2461 accgcgtcct ggacgatcag gtcgtagttt tcctgctcgg cgatgcgctt gatggcatcg
     2521 ttggccttgg tgacgatgga cgagaactct tcgttgcgac ggcggttgaa gtcttcctgg
     2581 aactcgcggc gcttgcgctg caggtccatg tcgaggttgg agagctcacg ctggcgcgtg
     2641 gtgcggtcgg agtcggacag cacggggctg tctttttcga actggtcggc cttggtgcgc
     2701 agggtattgg acaggcgcgt cagatcggcg tcgcgcgact tgaattcggc ctcgatcttg
     2761 ctttgtgcgg ccttggcagg gaccgattcg cgcaggatgc gctcggtgtt gacgaaaccg
     2821 atcttggtgc cttgcgaggg agccgtggcc gattgagcct gggccgtgcc cagggcggcc
     2881 gaacccatca gcaacgcagc ggccagggcc atggcgctgc ggcgcacgat gcgggattgg
     2941 gtcatacgtg caaagtcaga cattgaaact ctcacctgaa gtacgtgccg cgagcggcta
     3001 aaacatgaat cgaacctgcc attggcagta ttgtgccatg cccgaaggca tggcctttta
     3061 agacggatca gaaggccgtg cccacctgga attggaaggc ttccttgttg tcgccctgct
     3121 tggcgttgag cgcatggccg aaagacaatt gcagcgggcc cagcggcgac tgccacgaca
     3181 ggccgacacc ggcggaatag cgccagccgc aggggtccac cacctgggtc gtggccgagc
     3241 cgctggagca ggtcaggctg cccgtggtcg agacctggcc gccgtcgagg aacaggaacc
     3301 agcgcagggt gcggtcgtgg ctggcgcccg ggaacggcag gtacagctgt atgttggcgg
     3361 ccagcatgcg ttgaccaccg atatagtcgc cggtgagcgt atcgcgcggg cccagcgacg
     3421 agctttcgaa gccgcgcacc gaacccaggc cgccgccgta gacgttcttg atcaccggga
     3481 aaatattgcc gccgtagctg tggccgtagt tggccaggcc gttgaaggcc agggtgtacg
     3541 agcggttgag cgggatgaag tactgctgct gcgccgagaa catggtgtac ttcaggtcca
     3601 tcgtgccgac gtcggcgcgc agcttggtgt acgacccgcg ggtcggcgcc agcgcgctgt
     3661 cgcgggtgtc cttggaccag cccgcgttga gaatgaccgt attggtcttc tggccgtagg
     3721 cgttgacgaa gtcgtcgtag gcctgcggcg agtccgagta caggctgacg tcgttgcgct
     3781 cgaggttcgc gcccatgaag atgcggtcgg tctcggtaat gggcacgccg aagctcaggc
     3841 ccgcgccgat cgtgcgcacc gcatagttgc cggtgacgcc gacccagggg cgggtcaggc
     3901 ggtagtagag cgacaccgtg cggctgatgc cgtccttggt gaagtacgga tcggtctgct
     3961 cgagcacggc cgaggtctgc acgtcgctgg tgccgacgtt gaacgacatg ttggtgccgc
     4021 tgccgaagat gttgtcctgg ctgatgccgg ccgagaaaat ggccttgtcc gccgaaccgt
     4081 agccgatgcc caggttgatg atgccggtgg gcttttcctt gacgttgacg ttgaggtcga
     4141 cctggtcggg cgagttcggg acgggatcgg tcttgatgtc gacgtcggtg aagtagccca
     4201 ggcggtcgat gcggtcgcgc gaggtgcgga tttcgcccgc gtcgtaccag gcggcttcct
     4261 gctggcgcat ttcgcggcgg atgacctcgt cgcgggtgcg cgtgttgccg cccatctgga
     4321 tgcggcgcac atagacgcgg cggctgggat cgacgtagaa cgtgaggtcg gcctcgtgct
     4381 tctcgcggtc gagctgcggg ttggggttga cgttggcgaa ggcgtagccc agttcaccca
     4441 ggtagttggt gatggccttg gccgaattgt tggcctgctc ggcgttgaac accgtgcccg
     4501 gcttgatctg caccatcttc ttgatttcgt cgtcgaggcc gagcagattg ccggccagct
     4561 tgacgtcgcg caccttgtag ggctcgcctt cgtggatggt gaccgtgatg tagatgctct
     4621 ggcgatcggg cgagatggtg acctgcggcg gatcgttgga gtactccaga tagccgcggt
     4681 tgaggtagaa ggaacggatc ttgtcgatgt cgccttcgag cttttcgcgc gagtatttat
     4741 cggtgttggt gtaccaggta agccagcccg gcgtggtcat gtcgacttcg tcgagcagcg
     4801 tgctctcgga gaaggtcttg ttgcccacga agtggatttc gcggatcttg gcgacctcgc
     4861 cctcgaagat gtcgaaggcg atgcccacgc ggttgcgcgg cagcggcgtc accgtggcgg
     4921 tgacctcggt gctgtacttg cccttgctaa ggtattgctg cttgagctcg ttctcggcct
     4981 ggtcgagcat ggactggtcg aagatgcggc cttcggcaaa tcccaccttg gccagcgcgg
     5041 agacgatgtt cttggactcg aactcgcgca tgccggagaa ggtgatcgat gcgaccgtgg
     5101 ggcgttcctg cacggccacg atcaccacgt cgccttcggt gcggatctgc acgtcgctga
     5161 agaagttgga gccgtacagc cggcgcacgg cctcggaggc ctggtcgtcc gtgaagcgct
     5221 ggcccacctt gacgggcatg aagccgaaca ccgtgccggc gtcgacccgc tgcaagcctt
     5281 cgacacggat gtccttgacg acgaaaggtt cgaaagccaa cgcccacgtt ggtgcgaaaa
     5341 ggatgaccga agtcagcaaa tacctcagcg acgattggag cactcgctga aacattcggc
     5401 gaaaagacat aagctgacgt atccttaggt gggcggtaaa cggagcattt tattcgattc
     5461 gcgcctaggt gaacaggcgg gcgaagtcat tgaacagcgc gaggcccatc aaacaggcca
     5521 ataaacccat gccggcgcgc tggccgatct ccatccagcg atcgggcacc gggcgtccgc
     5581 gcacgatttc cagcaggtaa tacagcaggt ggccgccatc gagcatgggg atgggcagca
     5641 ggttgagcac gcccaggctg atgctgatca gcgccaggaa cgccacatag gccgccagcc
     5701 cgatgcgggc cgtctggccg gcatagtcgg cgatcgtcac cggcccgctg atattgcgcc
     5761 aggacacgtc gcccacgacc atccggccca tcatgcgcaa ggtcagccag gccgtgtccc
     5821 aggtgcgccg cgcgccgcgg cccacgctgt cgaacacgcc gtaccgcacg gtgaccatgg
     5881 gcaggtcgcc gcccagctgc acgccgatgc ggcccacctg ccggccgtcg acctgctcgg
     5941 catgcggcac cacgtgcagg gaaatcggca cgccgccgcg catgacggtc atgggcagcg
     6001 gttggccggc gtgctgctgg atatagccga ccagggcgga ggcgtcgagc tggccggcca
     6061 gctcgcccgc cgccgtgatc aggtcgccgg cctgcaggcc ggccgcctcg ccggcgctgc
     6121 cggcaatgac ctggcgcacg accgggcgcg gctgcgccag gtgaaagccg gcccaggcca
     6181 gcggatcgcc ctgctccggg tccatgtggt tgcctgaggc cagcaccagc gtgcgctgca
     6241 gcgcgccgcc ttcggccggc tgcacgtcca gcacggactt gccgcccgaa gacagcacat
     6301 cgatcaggtc ccagcgcgcg tcgtcccacg actccacggc tttcccgtcg accgcgatga
     6361 tccggtcgcc ctgccggaaa cccgcctgct cggccgggct gccggccgtg ggctggccca
     6421 ggatgggtgc cgggacttgc gcgccggcca tgtccagcgc ggcatagaac accaccgcca
     6481 gcaccaggtt gaataccggc cccgcggcca cgatggccat gcggcgccat accgactgct
     6541 tattgaaggc gcgggcagcc tcggccttgg aggcgcccgc cgggggatcg tcctgcatct
     6601 tgacgtagcc gcccagcgga atggccgaca cggcccattc ggtgccgtgc ctgtcggtac
     6661 ggcgcaggag cacccggccg aacccgatgg aaaagcgcag gacgcgcacg ccgcacaggc
     6721 gcgccacgcc gtaatggccg agttcgtgga aagtgatgag tatgcccagc gcaacggcaa
     6781 aggcaaccag ggtgaatagc atgtatcacc agtttagtcg ggtttcgggc aaaggccctg
     6841 cacggtggcg gcaaccacct caggccagcc ccagatcgcc tgcgtaagcg cgtgccgcgg
     6901 cgtcctgcgc cagcacgtct tgcagcgaac ccagcgtagc agatggctgc ccggcctgcc
     6961 actcgagcgt ggcctcgatg acgcgggcga tccagggata aggcaggcgg ccgtcgagaa
     7021 aggcctgcac ggccacttcg ttggcggcat tgagcgcgat gcaggcgtcc tgcccggcct
     7081 tgagcgcctc gaacgacagc cgcaggcagg gaaagcgcgt gaaatcgggc gcctcgaaat
     7141 ccaggcggcc cagccgggcc aggtccagcc cccctactcc gctggccagg cgctcgggaa
     7201 agcccaggcc gtaggaaatc ggggtacgca tgtcgggctg gccgagctgg gccagcaccg
     7261 agccgtcctc gtactcgacc atggaatgca ccacgctctg cggatgcacc accacgtcga
     7321 tccgctcggg cggcatggcg aataaccagt gcgcctcgat cacctccagg cccttgttga
     7381 gcatggtggc cgaatcgacg gagatcttgc ggcccatgct ccagttggga tgggcgcagg
     7441 cctgctcggg cgtgacgtcc tgcaggtctt cgagggccgt gcggcggaac ggcccgcccg
     7501 aggccgtcag cagcagccgg cgcacgcccg ccgccggctc ggcgggcgcg ccggcacgcc
     7561 cgccggacgc gccatggtgc ggcaggcact ggaagatggc attgtgttcg ctgtcgatgg
     7621 gcaggagttc ggcgccgctg tcgcgcacgg cctgcatgaa cagcgtgccc gcggccacca
     7681 gggcttcctt gttggccagc agcacgcgct tgccggcccg ggctgccgcc agggccgacg
     7741 gcagtccggc cgccccgacg atggccgcca tgaccatgtc gcaaccggcc tcggccgcgg
     7801 tctcggccag cgcctgcgcg cccacgcgga tctcgggcaa ggccgcgccg cgccaggccg
     7861 ccgtgaatcg ctcgcgcgcc gcatcgtcgg gcaccagcac cacgggcgcg cggcaggccg
     7921 cggcttgctc ggccaggctt tccatgcggc tatgcgccga caaggcatgc acttgcaggc
     7981 gctcggggtg gcgcgcaatg acgtccagcg tgctgcgtcc tacggaaccc gtcgagccca
     8041 gcacgacgac gcgctgcggc cgacctgact gcgatatgtt caaaacagag ctcccgaaac
     8101 gagcaaggcg atcggcacga ccggcagaat ggcgtcgagg cggtcgtaca cgccgccgtg
     8161 gcccggcagc agcccgctgg aatccttgac gccggcgcgg cgcttgagca gcgattcgaa
     8221 caagtcgccc atgatggaga cggctcccag gaaggctgcg atgagaatag cgccccagat
     8281 cgaccagcgg tcgaccagca tgtagccgaa ggtgtccggc caccaggcgc cgaccagggt
     8341 gacgacgacc gcgcccacca ggcccgatag ggcgccctcg accgtcttgc ccgggctcac
     8401 gcggggcgcc agcttgcggc ggccgaattt gcgcccgccg aaataggcgc agatgtccgc
     8461 ggcccagacc agggccagca gcgtcagcac atacagcggc ccgaaggcca gatacatcac
     8521 ggtcagcacg gcccaggccg tgaccgtggc cagcaccgcg aacgccgacc agcccacgct
     8581 ggcggggggg gcgtcggagc ggccgcgcac cacggcgatc acggccggga tcaaccagat
     8641 caggctgacg atgaccacca gcacgccgaa ggccaggagc tcttcaggca gcacgccgcc
     8701 cgtgggcgcg ctccagaccc acgacagggc cagcaggaag gcaaagacga cgatgccgat
     8761 cacacgcggc acggcggccg gcacacgtgc cggcagcgtc aggcgcagcc attcccagca
     8821 ggcgcaggcc gccgccaggg acagcagcgc cagcatccag acgggctgcg gcgccgcgat
     8881 ggcggcggcg aggacgatga gcagaacgac ggcggtgatg atacgttggc ctaacatcgc
     8941 aggaaagtcc gtggtgatag tccgtggggg ggtatcagcg cgtcgccagc acttgcgcgc
     9001 tggttcggcc aaaacggcgc tcgcgcccgc tgtaccacgc gatggcctct tcgagatcgg
     9061 ccccgtcgaa gtcgggccaa taggcatcgg tgaaatacag ttcggtgtag gcgagctgcc
     9121 agatcatgaa attggaaatg cgctgttcgc cgccggtgcg gatgaacaga tcgggctcgg
     9181 gcgcccacga catggagaga tgctcggcca gcgcagtctc ctcgatttgc tcgggatgcc
     9241 cggccaggcc ggggttggcg cgcagcatgg cgcgcatggc ctgcagcacg tcccagcggc
     9301 cgccatagtt ggccgcgatg gacaggcaca gcttgtcgtt gcactcggtg cgggcctcgg
     9361 cctggtggat caggtcctgc aggcgcggct cgaagggcga cagatcgccc accacgcgca
     9421 ggcgcacacc ctgtttttcc agcttgccga cttcgcgctc gagcgagcgc acgaacaggc
     9481 gcatcagcag cgagacctcg tcggccggac gccgccagtt ttcggaactg aaggcgaaca
     9541 gcgtgagata gcgcacgccc aggcggccgc agctctcgac ggcgcggcgc acggcctgca
     9601 cgcccttgac gtggccggcc gtgcgcggca ggtgccggcg cgtggcccag cggccattgc
     9661 catccatgat gatggcgatg tgctgcggca caccggaaac ctccggtacg acctgggtgg
     9721 aactgatgac catgggcgcg tcgagtcgga ctggcgttgc ggcgggcgcc gcctacaccg
     9781 tcatgatttc ggcttctttc tgggcgatca gcttgtcgat gtcggcaacg tagcgatcgg
     9841 tgagcttctg cacgtcgtcc tgggcccggc gttcctcgtc ttcggaaatg accttgtcct
     9901 tgaccagttt cttcaggccg tcgttggctt cgcggcgcag gttgcgcacg gccaccttgg
     9961 cgtcctcgcc ttcgctgcgc gccaccttgg tgaggtcgcg gcgacgctct tcggtgagcg
    10021 cgggcatggg cacgcggatg gtgtcgccca gcgccatggg gttcaggccc aggtcggatt
    10081 cacggatggc tttctcgatg ggggccagca tggacttttc gtagggctgc acgctgaccg
    10141 tgcgggcgtc gaccagggtc aggttggcca cctggcccac cggcacgggc gaaccgtagt
    10201 actcgacgtg cacgtggtcc aggatgctgg ggtgcgcgcg accggtgcgc agcttgccca
    10261 gattggcctt gagggtgtcc agcgacttgg tcatccggct gtcggcggat tgacggatat
    10321 ctgcaacgct catggagatt tccttgtttt atac
//
LOCUS       DASWPO010000136         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2083337, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000136 DASWPO010000000
VERSION     DASWPO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083337"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..402
                     /gene="rplR"
                     /locus_tag="VGC69_07600"
                     /pseudo
     CDS             37..402
                     /gene="rplR"
                     /locus_tag="VGC69_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434799.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            418..939
                     /gene="rpsE"
                     /locus_tag="VGC69_07605"
     CDS             418..939
                     /gene="rpsE"
                     /locus_tag="VGC69_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY0295184.1"
                     /translation="MAKVQSKNAAEKENDDGLREKMIAVNRVSKVVKGGRTMSFAALT
                     VVGDGDGRIGMGKGKAREVPVSVQKAMEQARRGMFKVALKNGTLHHTVVGKHGASTVL
                     ISPAAEGTGVIAGGPMRAIFEVMGVRNVVAKSLGSSNPYNMVRATLNGLRASMTPSEI
                     AAKRGKTVEEILG"
     gene            943..1128
                     /gene="rpmD"
                     /locus_tag="VGC69_07610"
     CDS             943..1128
                     /gene="rpmD"
                     /locus_tag="VGC69_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY0295185.1"
                     /translation="MAQKQIKVTLVRSVIGTKADHRATIKGLGLGKVNSSKVLIDTPE
                     VRGMIRKVDYLVSVSEV"
     gene            1138..1572
                     /gene="rplO"
                     /locus_tag="VGC69_07615"
     CDS             1138..1572
                     /gene="rplO"
                     /locus_tag="VGC69_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY0295186.1"
                     /translation="MQLNSLSPALGSKHAKRRVGRGIGSGLGKTAGRGHKGQKSRSGG
                     FHKIGFEGGQMPLQRRLPKRGFTPLGQHLYAQIRLTDLQDLPIDEIDVQALKAAGVVG
                     QQVRYAKVIKAGELSRKVVLRGIAATAGARAVIEAAGGSIAE"
     gene            1586..2911
                     /gene="secY"
                     /locus_tag="VGC69_07620"
     CDS             1586..2911
                     /gene="secY"
                     /locus_tag="VGC69_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY0295187.1"
                     /translation="MAKAQAPGKSGTRYGDLKRRLVFLVLALIVYRLGTHIPVPGINP
                     DALSDLFRQNQGGILGLFNMFSGGALSRFSVFALGIMPYISASIIMQLMSVVMPSLEA
                     LKKEGEAGRRKITQYTRYATVLLALVQAVGISVALESQPGLVIDPGMVFRVTTVVTLV
                     TGTMFVMWLGEQITERGLGNGISILIFGGIVAGLPTSLAGLLDLVRTNSMSVLSALFI
                     IALVVLVTAFVVFVERGQRKITVNYAKRQVGNKVYGGQSSHLPLKLNMAGVIPPIFAS
                     SIILFPATITSWFSSSDKLVWLRDLAAALQPRQPLYITLYSVAIIFFCFFYTALVFNS
                     RETADNLKKSGAFVPGIRPGEQTARYIDKILMRLTLAGGLYITLVCLLPEFLVMRWNV
                     PFNFGGTSLLIIVVVTMDFMAQVQAYMMSHQYDSLLKKANFKGAGLPLR"
     gene            2922..3140
                     /gene="infA"
                     /locus_tag="VGC69_07625"
     CDS             2922..3140
                     /gene="infA"
                     /locus_tag="VGC69_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0295188.1"
                     /translation="MSKDDVIQMQGEVLENLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRARIVFRAK"
     gene            3196..3309
                     /gene="rpmJ"
                     /locus_tag="VGC69_07630"
     CDS             3196..3309
                     /gene="rpmJ"
                     /locus_tag="VGC69_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY0295189.1"
                     /translation="MKVSASVKKMCRNCKVIRRNGVVRVICTDPRHKQRQG"
BASE COUNT          648 a         1025 c         1034 g          625 t
ORIGIN      
        1 accaagaaga aataacggcg cacgcaagga cgaatcatgg acaaaaaagt ttcccgtttg
       61 cgtcgtgcgg ttccgacccg ccggaagatc aacgagttgc gcgttcaccg cctctcggtc
      121 ttccgctaga accagcacat ctacgccaac atcatttcgc cggaaggcga ccgtgtgctg
      181 gtcagcacct cgacgctgga agccgaagtg cgcgccgaac tggccggcaa gaccggcctg
      241 ggcggcaaca ccgccgctgc cgcattggtg ggcaagcgcg ttgccgagaa ggccaaggcc
      301 gccggcatcg agctggttgc cttcgaccgc tcgggcttcc gctaccacgg ccgcgtcaag
      361 gccctggccg acgccgcccg tgaagccggc ctgaagttct aagcgaggat cagtcaaatg
      421 gctaaggtac aaagcaagaa cgccgccgag aaagaaaacg acgacggtct gcgcgaaaaa
      481 atgatcgcgg tcaaccgcgt gagcaaagtc gtcaagggtg gtcgcaccat gagctttgcc
      541 gcgctgaccg tggtcggcga cggcgacggc cgcatcggca tgggcaaggg caaggcgcgc
      601 gaagtgccgg tctcggtcca gaaggcgatg gaacaggctc gccgcggcat gttcaaggtt
      661 gccctgaaga acggcacgct gcatcacacc gtggtcggca agcacggcgc ctcgacggtg
      721 ctgatctcgc ccgccgccga aggtaccggc gtgatcgccg gcggcccgat gcgcgccatc
      781 ttcgaagtga tgggcgtgcg taacgtggtt gccaagagcc tcggctcgag caacccctac
      841 aacatggttc gcgccacgct caacggcctg cgcgcttcca tgacgccgtc ggaaattgct
      901 gccaagcgcg gcaagaccgt cgaagagatc ctggggtaaa tcatggctca gaagcagatc
      961 aaagtcaccc tcgtgcgctc cgtgatcggc accaaagccg accaccgcgc cacgatcaag
     1021 ggcctgggcc tgggcaaggt caatagcagc aaagtactga tcgacacccc cgaggtgcgc
     1081 gggatgattc gcaaagtgga ttatctcgtc tccgtctcgg aagtctaagg aacggacatg
     1141 caactcaatt cgctctcgcc cgctctgggc agcaagcacg ccaagcgccg cgtcggccgt
     1201 ggcatcggtt cgggtctggg caagaccgcc ggccgtggcc acaagggtca gaagtcgcgt
     1261 tcgggcggct tccacaagat tggtttcgaa ggcggccaga tgccgctgca gcgtcgcctg
     1321 cccaagcgtg gcttcacgcc cctgggtcag cacctgtacg cccagatccg cctgacggac
     1381 ctgcaggacc tgcccatcga cgaaatcgat gtgcaagccc tgaaggccgc cggcgtggtc
     1441 ggccagcaag tgcgctatgc caaggtgatc aaggcgggcg aactgtcgcg caaggttgtc
     1501 ctgcgcggta tcgctgcgac ggccggcgct cgcgccgtga tcgaagcggc cggcggttcg
     1561 atcgctgaat aagcagaggt gacgtgtggc taaagcacag gctccaggca agtccggtac
     1621 gcgatacggc gatctcaagc gccgtctcgt gttcctggtg ctcgccctga tcgtctatcg
     1681 cctgggcacg cacattcccg ttccgggcat caatcccgat gcgctgtcgg acttgttccg
     1741 ccagaaccag ggcgggatcc tcggcctgtt caatatgttc tcgggcggcg cgctgtcgcg
     1801 cttctccgtg ttcgccctgg ggatcatgcc gtacatctcc gcctccatca tcatgcagct
     1861 gatgtcggtg gtgatgccct cgctggaagc cctcaagaaa gagggcgagg cgggccggcg
     1921 caagatcacc cagtacaccc gctacgccac ggtcctgctg gcgctggtgc aggcggtggg
     1981 catctcggtc gcgctggagt cgcagcccgg cctggtcatc gatccgggca tggtgttccg
     2041 cgtcacgacg gtggtcacgc tggttaccgg caccatgttc gtcatgtggc tgggcgagca
     2101 gatcaccgag cgcggcctgg gcaacggcat ctcgatcctg atcttcggcg gtatcgtcgc
     2161 gggcttgccc acgtcgctgg ccggcctgct cgacctggtg cgcaccaact cgatgtccgt
     2221 gctgtcggcg ctgttcatca tcgccctggt ggtgctggtg acggccttcg tggtgttcgt
     2281 cgaacgcggc cagcgcaaga tcacggtgaa ctacgccaag cgccaggtgg gcaacaaggt
     2341 gtacggcggc cagagctcgc acctgccgct caagctgaac atggccgggg tgattccgcc
     2401 gatcttcgcg tcgtcgatca tcctgttccc ggccacgatc acgagctggt tctccagcag
     2461 cgacaagctg gtgtggctgc gcgacctggc ggctgccctg cagccccgcc agccgctcta
     2521 catcaccctg tactcggttg cgatcatctt cttctgcttc ttctacacgg ccctggtgtt
     2581 caacagccgc gaaacggcag acaacctgaa gaagagcggt gctttcgtgc ccgggatccg
     2641 tcccggtgag caaacggcgc gctacatcga caagattctg atgcgcctga ctttggccgg
     2701 cggcctgtac atcacgctgg tgtgcctgct gcccgagttc ctggtcatgc gctggaatgt
     2761 cccgtttaat tttggtggta cgtctttgtt gattattgtt gttgttacga tggacttcat
     2821 ggctcaggtg caagcctaca tgatgtctca ccagtacgac tcgctgctca agaaggccaa
     2881 cttcaagggt gcgggcctgc cgttgcgata aacagagaat aatgtccaag gacgacgtca
     2941 ttcagatgca aggtgaggtt ctcgagaacc ttcccaacgc gacttttcgc gtcaagcttg
     3001 aaaatggcca cgtagtactg ggtcatattt ccggcaagat gcgcatgcat tacatccgga
     3061 tcctgcctgg cgacaaggtg accgtcgaac tcactccgta cgatctgagc cgcgcgcgga
     3121 tcgtgttcag agcgaagtga acggctcgag tgaacgcacg tcgttctgcc ggttttgagt
     3181 agtcaaggag aaatcatgaa agtgtcggca tcggttaaga aaatgtgtcg caactgcaaa
     3241 gtcatacgcc gcaacggagt tgtgcgcgtc atctgcactg accctcgcca caagcagcgt
     3301 caaggctaaa gcggcatcgt cagaagctaa gg
//
LOCUS       DASWPO010000137        20480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_387389, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000137 DASWPO010000000
VERSION     DASWPO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20480
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387389"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2174)
                     /locus_tag="VGC69_07635"
     CDS             complement(<1..2174)
                     /locus_tag="VGC69_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEY0295190.1"
                     /translation="MAAPDSRSATTPDQPPYADPTLNPWLAAWQYGVDAWQRGVLFTD
                     VMRQRGNQYRQHIAKKAPNVLSMNSEVVLDGRGFERPVNYALLRIIPPEGVVIDPIKR
                     PFLVVDPRAGHGPGIGGFKPESEIGVAIRAGHPCYFASFLPRPTATQTVEDVMHAEAH
                     FLEEIIALHPQAEGKPVVVANCQAGWQIMMTAAMRPELFGPIIIAGAPLSYWAGWRGM
                     NPMRYSGGLLGGSWMTALASDLGNGTFDGAWLVQNFESLNPANTLWDKQYNLYANIDT
                     ETQRYLGFEKWWGGHVYLNGPEIQYIVDNLFVGNRLATAGLVTSDGIRIDLRNIQSPI
                     IVFCSKGDNITPPPQALGWITDLYQNDEEIQAHGQTIVYAVHESIGHLGIFVSGSVAR
                     KEHQEFASNIDMIDVLPPGIYEAEFAERTADTPNAETAGDYVMSVENRTLDDVRTIVQ
                     QIPEDDKRFATVSRISQINLGLYRSFVQPWVRTAVTPQSAEWMQRLHPLRLPYELISD
                     QNPAIAPVAQLAEAVREQREPVPADNPFLVAQNMMSDWIETSLNSYQETRDAAVERLF
                     MNLYGSPLMQDWAGLGAKDGPARRHPGISPEHRSFMAKRKTELLALVPVGGLHRAAIR
                     MLLYVIGADGGLDERSFAVIRRLRTRDGQAIASLERFKELMREQTMVMRLDPAGSLRA
                     MPKLLSKATPEDIRAMLASMKEVLDAAQPLNERARTSLREMER"
     gene            complement(2300..2743)
                     /gene="cynS"
                     /locus_tag="VGC69_07640"
     CDS             complement(2300..2743)
                     /gene="cynS"
                     /locus_tag="VGC69_07640"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEY0295191.1"
                     /translation="MTRNDVTELVVAQKIAKKLKWEDLARAVGQSKEWTTAALLGQMT
                     LTEKQAKAAGKLLELPKEAVALLQVVPYKGSLPTAVPTDPLIYRFYELVSVYGSTFKA
                     LIHEEFGDGIMSAIDFKMDISREADPKGDRVRIILSGKFLPYKTY"
     gene            complement(2788..3414)
                     /gene="pncA"
                     /locus_tag="VGC69_07645"
     CDS             complement(2788..3414)
                     /gene="pncA"
                     /locus_tag="VGC69_07645"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEY0295192.1"
                     /translation="MSTFADDTALIVVDLQPDFMPGGPLACHEGDAIVADIDALLRAR
                     RYRTVVATQDWHPADHVSFASRHAGAAPFQAITLHGQPQTLWPDHCVQGTPGAALHGQ
                     VDWSACDLILRKGTNPQVDSYSAFRENHGPGGIRPATGLAGWLRERGIAEVHVCGLAR
                     DYCVLWTAQDAAQAGFRVRFLWDLTRPVSPDGDAPARAALQAAGIAQA"
     gene            complement(3435..3920)
                     /locus_tag="VGC69_07650"
     CDS             complement(3435..3920)
                     /locus_tag="VGC69_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0295193.1"
                     /translation="MLDIIDIDFKNPEHGRIVVGLLDEYASGDMGGNEPLSEFSRGNL
                     VASLAARPWAHALAARVDGQPAGLAIYLEGFSTFACKPLLNLHDFMVSERFRGQGVAQ
                     KLLAALEDRARAMGCCKLTLEVLEGNIPARTLYQKMGFEGYQLQDEHGRALFWQKKLA
                     A"
     gene            4044..4685
                     /gene="mog"
                     /locus_tag="VGC69_07655"
     CDS             4044..4685
                     /gene="mog"
                     /locus_tag="VGC69_07655"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEY0295194.1"
                     /translation="MTPTTASASARIARRHPDELIVGLVSVSDRASAGVYADEGVPSL
                     QAWLGTALASPWQAVERLIPDDAAGITAALIDLVDHCGCDLVLTTGGTGPARRDVTPE
                     ATLAAGTREMPGFGEQMRQISLKFVPTAILSRQVAVIRETASHAALIINLPGQPKAIR
                     ETLEGLRGGDGAVQVPGIFAAVPYCIDLIGGPYAETRPEVIQAFRPKSARRGA"
     gene            4697..5866
                     /locus_tag="VGC69_07660"
     CDS             4697..5866
                     /locus_tag="VGC69_07660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY0295195.1"
                     /translation="MRDRRHWLTRERIVLYGTLMLVFFLAAAIMNVKTSRDGLVAGTH
                     RPLGSDFIAFWGVSHLALSGHAASAYDFDMRALTHAEQVAVPASDVSYPWNYPPAFVL
                     LILHLALMPYFVAFWVFMATTLAMYVLAFWRAVGQGLGLWCLAIFPAAWINFGGGQNG
                     FLTAALAGGALLCLPRRPVMSGVLIGLLSIKPHLAVLFPLALVAAGAWRTIAVAAATG
                     LAVNALGTAVLGTDVLWAFWRGLGHAQGMLAAGERFYWTKIPSVFVMVRSLGGAASLA
                     YAVHAAAAAVAAWVVWRVWRASGDWMLRGAALMTATFFISPYDYNYDLVWLAFPIAWL
                     ALVAQREGWRALDREILLAVALWPLLWFFPDWAFKVQLSPIPLAGLLWVIRRRVS"
     gene            complement(5863..6168)
                     /locus_tag="VGC69_07665"
     CDS             complement(5863..6168)
                     /locus_tag="VGC69_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295196.1"
                     /translation="MAKIPTSPSDALRQAADRLALARRAFERGERGLRQLRRSRVPFI
                     SSLRNTGLTYIQARSKYDTCLDEQQRLHQQTTHALHYAERLYGALCAQLDLEQKKAA"
     gene            6370..7434
                     /gene="fba"
                     /locus_tag="VGC69_07670"
     CDS             6370..7434
                     /gene="fba"
                     /locus_tag="VGC69_07670"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395676.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY0295197.1"
                     /translation="MALVSMRQLLDHAAEHGYGIPAFNVNNLEQVQAIMEAAAETDSP
                     VIMQASAGARKYAGEGFLKLLIQAAVQTYPHIPVVMHQDHGQSPDVCQGAINLGFSSV
                     MMDGSLMSDGKSVASYDYNVDVTRKVVDMAHKLGVTVEGELGVLGSLETMKGDKEDGH
                     GAEGTMTREQLLTDVEQAADFVKQTQCDALAIAIGTSHGAYKFSKKPTGDILSIQRIK
                     EIHARIPNTHLVMHGSSSVPQELLAEIRQFGGDMKETYGVPVEEIQEGIKYGVRKINI
                     DTDIRLAMTAAIRRFFAENPAKFDPREYLKPAKAAAKAVCVARYQQFGTAGNASKIKA
                     VSLADMAADYACGKLAQVVQ"
     gene            7527..8408
                     /locus_tag="VGC69_07675"
     CDS             7527..8408
                     /locus_tag="VGC69_07675"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY0295198.1"
                     /translation="MTNALHQSTIKSLPLLGRGKVRDMYAVGDDKLLIVASDRISAFD
                     VILDDPIPGKGQVLTELTEFWLKKLGHLIPTHVTGIKPEDVVAPDEVDQVRGRAMVVK
                     RLKPVLVEAVARGYLIGSGWKDYQETGSICGIPLPAGLKQAGKLPQPIFTPAAKAEFG
                     MHDENVDFAHVVKEVGQEMAEKIRDITIKLYVEAAAFAATKGIMIADTKFEFGLDEQG
                     TLYLMDEVLTPDSSRFWPADGYREGISPPSFDKQFVRDWLETQPWGKTPPAPRLPKDV
                     LEKTAAKYREALDRLTA"
     gene            8528..8680
                     /locus_tag="VGC69_07680"
     CDS             8528..8680
                     /locus_tag="VGC69_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5993 family protein"
                     /protein_id="HEY0295199.1"
                     /translation="MVFPFLTGALAICYGMLGKRPACFVLWLITLGLFAGWAYQHLDG
                     PLPLSL"
     gene            8696..9238
                     /locus_tag="VGC69_07685"
     CDS             8696..9238
                     /locus_tag="VGC69_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY0295200.1"
                     /translation="MALFLQNPGHGSRQFNALLLFLICASLVFSLVWQGLAVDYACPM
                     CALQRVALVLAGVGLLLNVRLGPSPLHYAMTIAAALAGAAISGWLALRPADLGARLLF
                     GLHLYSWAFLVFGALVVFSLLMLALDRRWGDNALKCPVSMLGAVVMALFLAAALASAA
                     GTSLDCGVAACLKQLAYAAR"
     gene            complement(9260..10456)
                     /locus_tag="VGC69_07690"
     CDS             complement(9260..10456)
                     /locus_tag="VGC69_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEY0295201.1"
                     /translation="MTSASSPKRRWWGVIALAFSAFIFNTSEFVPVGLLHAIGASFDM
                     PANDVGLMLTLYAWAVALLSLPGMLLTGQAERRKLLTLVFVLFIASHVLAGVAWSFPV
                     LLLGRLGIAGAHAIFWSISVALAVRMAPEGRKGLALGLIATGTSMAMVLGIPLGRVVG
                     EVLGWRTTFLAIAVVASLILAVLRATLPELPSQNAGSLASLPSLFRRPTLVLLYVLTA
                     VAISAHFTAYTYIEPFVYQVAKLGDNAVTAMLLLFGIAGIPGAMFFNRHYARQPKRFL
                     LATVAVLTACLLTLRPSAASPAVLSMHALLWGAAIICFNLAMQAWILRLASDATDLAM
                     SLFSGIYNIGIGTGALLGNLIANHVSVAYVGWFGGSLGLVSVCVCWMAFQVRMDAPPR
                     MSSPTV"
     gene            complement(10716..11633)
                     /locus_tag="VGC69_07695"
     CDS             complement(10716..11633)
                     /locus_tag="VGC69_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HEY0295202.1"
                     /translation="MSAPASPTASSMSATVIDLPLRDWRSPDAAGLSAQALHALEQGQ
                     VLHFPHLGFALTEAEQALLDPALTDPKRKNISLTPKGLAGTVATGAARDRVQDVLQRY
                     LDSTGALLDALFPGYRDHRHSPATSLRLHAIGTWHPSWRKDDRLLHVDAFPSRPLHGE
                     RILRVFTNINPRNEPRTWRVGGSFEASARHYLPGMNKPWRPWLAGLMHKAGITKRRRT
                     EYDHLMLQMHDAMKRDERYQREGDWTQVDFMPGSTWVCFSDQVPHAAMGGQFMLEQTW
                     QLPLAALGYAELAPVRVLEKLAKRPLLAA"
     gene            11712..12242
                     /gene="purE"
                     /locus_tag="VGC69_07700"
     CDS             11712..12242
                     /gene="purE"
                     /locus_tag="VGC69_07700"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY0295203.1"
                     /translation="MAKTDSTAQDATQEATPVVGVIMGSSSDWDVMQHAVAMLRDFGV
                     PCEAKVISAHRMPQDMADYGAQAAGRGLRAIIAGAGGAAHLPGMMAALTEVPVFGVPV
                     PSKYLRGEDSLLSIVQMPKGIPVATFAIGEAGAANAALHVIANLATTDDALRRRLAAF
                     RASQTQAARAMTLPGA"
     gene            12246..13421
                     /locus_tag="VGC69_07705"
     CDS             12246..13421
                     /locus_tag="VGC69_07705"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY0295204.1"
                     /translation="MRDVEPIAPGGWLGLLGGGQLGRMFCHAAQSLGYKVAVLDPAAD
                     GPAAMVADRHIQAAYDDAAGLAALAELCPAVTTEFENVPADSLRALSTRCRVSPAADA
                     VAVVQDRIAEKTFIASQGIPVAPHAAVRGEADLRAAPDALFPGILKVARLGYDGKGQA
                     RVQTRAEALAAFAAFQGVPCVLEALLPLDYEISVVLARGFDGADVAYPVSRNAHRDGI
                     LAVSTVAGDDCPSPHREAATRAARAIAQGLGYHGVLCVEFFVLADGRLLVNEIAPRPH
                     NSGHYSIDACVTSQFEQQARVMACLPLGSTRVVAPSVMLNILGDVWFPAGAGTAREPD
                     WPAALAASSSAKLHLYGKREARRGRKMGHITIVGETLAQAQADAARVAAALGMAAPQ"
     gene            13427..14482
                     /locus_tag="VGC69_07710"
     CDS             13427..14482
                     /locus_tag="VGC69_07710"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0295205.1"
                     /translation="MNAPAPQPSRHDIEQAARRLFAGQLVAFPTETVYGLGGDAENPQ
                     AVARIYAAKGRPANHPVIVHLAPEGDPAYWAAQIPPEARKLMQAFWPGPLTLILKRAP
                     HIPDAVSGGQDSVGLRCPSHPVAQALLRAFAALKASGQGGVAAPSANKFGQVSPTLAA
                     HVRGEFPDEVAQGMPVLDGGAAEVGIESTILDLSRLDQGVGAVLLRPGHISAAQIAAV
                     LGQPVHRPDAAAPRASGTLKAHYAPHTPLELIDDARLARVVQGAGLPAGRCVLVSYAE
                     LPPLPADLAARVQVRAVPADPAHYAQALYATLRELDGGGYARLLVQAPPRAPAWDAVN
                     DRIGRAAAAFTLDTAGL"
     gene            complement(14504..15460)
                     /locus_tag="VGC69_07715"
     CDS             complement(14504..15460)
                     /locus_tag="VGC69_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295206.1"
                     /translation="MSPRPALGLTRPTTLHQPSFAQLSVFNAVMVTGSVSRAAKLLGM
                     TQSGASRMLQQLEQDTGLRLFERAHQRLTPTQQAHQLQASVGRLRDAYGAVQRAIGGL
                     ADPDNGTVTVAAIPTQATTFLPEAIRRLRQRFPGITPSVEILANPPIVDLVQNGRVDF
                     GLVHDITPSPDTHNEDIGLQHVVCAAPRGHRFESLDCVTPQDLKPETFLSYGPQTNFG
                     YLVESAFTEAGIHMPMAVEATSSAALLALIRAGVGVGLVEPAAIVPFPLDAFVIRPFR
                     PTLCMYSRIIRSRARPLTRHAELLIEEYRAVVTGPQPAYFQA"
     gene            15609..16730
                     /locus_tag="VGC69_07720"
     CDS             15609..16730
                     /locus_tag="VGC69_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295207.1"
                     /translation="MSKPVADPCLLDAAQRALSDPGLGSERRRQLLGMLGAAGLTALL
                     PAGQAQADAGKIVVCNWGGDALKYQPQAWGQPFEKASGMKVEFDGTGPTFGKIRTMVE
                     SNHVVWDVCDANLAVAHSLGKQGLLEPIDYRIVDRNKVRPGFSSEYGVSHFIYSFVLA
                     FSKKAFPKKAPNTWADFWNVKDFPGTRILPDRILASLEAALMSDGVPADASKIYPIDE
                     KRALSRIAAIKDNAIFWKTAADSQQLLRSGEAVMGLIWHTRAVLLRREPGSDIDFTWN
                     QGILIPAGWLVPKNNPAGRKVFDFIASTQSPESQIELLKLMGNGPANPAASAMVPPEL
                     RKDNPTDDDNFRLQVVADSTWHGENQDRVYNDYVKVITG"
     gene            16744..18378
                     /locus_tag="VGC69_07725"
     CDS             16744..18378
                     /locus_tag="VGC69_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0295208.1"
                     /translation="MSFHADLVLHGGRIATLDPVLGVVQAVAIRAGRIVGAGSDADCL
                     NLAGPGTIKADLRGAFAMPGIHDSHCHPDGQAVKQGRWEDLGELSSAEALRQRIADFH
                     ARMPAGAWCLGFRFDETRIGGYPGRAMLDAAAPGRPVFLLRRDAQVGLANAAAFGLLR
                     DSGLADRVPGHCLEPAGGMARGRGVFAFTTLIAASDRIEDYLDGYPVVFREMARLGIT
                     SVHNALTGRLAVQAYRRLNAGRALPVRLGMMLNGRDQQLVDEVLEAGLRFGDGDETLR
                     LLGVEYGSDGSTSGRTAAYYAPYAGQDGGDGQPPNRGDVNYEADDLAAKVARIMAAGL
                     QVAATGNGDRGIDFALDAFEKGLVANPRAVPPRIEHCCCAPAATQARMARLGVIDSSA
                     TGFLYSLGDAYLRNRPEPDMAWLWPHRDMMRRGVLVCGHSDAPVCERNPFLGIWSMVT
                     RKTASGAVIGAHQALSIDEALRCYTVNPARAEGLAAGKGTLSPGKLADLIVLDRDPYA
                     IEADELRHVCVRATLLGGKAVYRDGGLDAALPEAAW"
     gene            18372..19229
                     /locus_tag="VGC69_07730"
     CDS             18372..19229
                     /locus_tag="VGC69_07730"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295209.1"
                     /translation="MVSRPALRASQAVLPGLLVLPVVVLVGGLFLAPVLRVLSLSFIE
                     PVPGLGNYAHLFAEPLYLRIMATTLRVCLWTTALSLLLAYVLAYVMANVGERARSWML
                     IGLLVPFWLSVLVRAFAWTMLLSRTGVVNATLMALGVIDSPLLLLRNETGVLIGMVHY
                     MVPYAVLPMLTAMLGIDRALTAAARALGAGPVRTFVSVYLPLSMPGVLAAAVLVFIFS
                     MGFFITPALLGGGKVMMVAQYIEFGISESTNWGIATAFASVLLGAVICSLLLVSRFVR
                     IPSLFGAAS"
     gene            19226..20029
                     /locus_tag="VGC69_07735"
     CDS             19226..20029
                     /locus_tag="VGC69_07735"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295210.1"
                     /translation="MTVRPSWWFRLAQVLALLVLAYLLLPGLAVIPVSFTDRSYLSFP
                     AQHWSLQYYRQLIDSPAWRDSALQSIMVAAISTALATLLGSLAAIGAWRLGGRLGGLV
                     RLLALVPLIVPTIVGALAYYRLYIDLGLLDTFAGVVLTHVIAAMPFVFMSVGSALSNF
                     DPRLEQAARSMGARPWQVLRRVTLPAIRPGVWSGALFAFIVSWDEIVVLLFVTTRDVY
                     LLPRAMWDGINQNVDPSIAAVATLMIAVTFACLCVQRLLVGRRVRRESP"
     gene            20026..>20480
                     /locus_tag="VGC69_07740"
     CDS             20026..>20480
                     /locus_tag="VGC69_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295211.1"
                     /translation="MSQFSAPDRALLRVQGLHKSFGAVKALDGVDFSLAPGEFLTLLG
                     PSGSGKTTTLMTIAGFVEPTGGRVLFEERDITRRLPEERDFGMVFQGYALFPHMTVEQ
                     NIRFPLAIRGVPRGQADRRVRQALEQVQLGAYAKRHPRQLSGGQQQRVAL"
BASE COUNT         3028 a         6895 c         7156 g         3401 t
ORIGIN      
        1 cgctccatct cgcgcaggct ggtgcgggcc cgttcgttca ggggctgcgc ggcgtcgagc
       61 acttccttca tcgaggccag catggcgcgg atgtcctcgg gcgtggcctt ggacagcagc
      121 ttgggcatgg cgcgcagcga accggccggg tccagccgca tcaccatggt ctgctcgcgc
      181 atcagttcct tgaagcgctc cagcgaggcg atggcctggc cgtcccgggt gcgcaggcgg
      241 cggatgacgg cgaagctgcg ctcgtccagc ccgccgtccg cccctatcac atacagcagc
      301 atgcggatgg ccgcgcgatg caggccgccg accggcacca gcgccagcag ttcggtcttg
      361 cgcttggcca tgaagctgcg gtgctcgggc gagatgccgg ggtgcctgcg cgccggtccg
      421 tccttggcgc ccaggccggc ccagtcctgc atcagcggcg agccgtagag gttcatgaac
      481 agccgttcca ccgccgcgtc acgcgtttcc tggtagctgt tcaggctggt ttcgatccag
      541 tccgacatca tgttctgcgc gacgaggaag ggattgtcgg ccggcacggg ctcgcgctgt
      601 tcgcgcacgg cctcggccag ctgggccacc ggcgcgatcg cgggattctg gtcggagatc
      661 agctcgtagg gcaggcgcag cgggtgcagg cgctgcatcc attcggccga ctgcggcgtg
      721 accgcggtac gcacccaggg ctgcacgaag ctgcggtaca ggcccaggtt gatctgcgag
      781 atgcgcgaca ccgtggcaaa gcgcttgtcg tcttccggga tctgctggac gatggtccgc
      841 acgtcgtcca gcgtgcggtt ctcgaccgac atcacgtagt cgccggcggt ctcggcgttg
      901 ggcgtgtcgg ccgtgcgttc ggcgaattcg gcttcataga tgccgggagg cagcacgtcg
      961 atcatgtcga tgttgctggc gaactcctgg tgttccttgc gggccacgct gcccgacacg
     1021 aagatgccca ggtggccgat gctttcgtgc acggcataga cgatggtctg gccgtgggcc
     1081 tggatttcct cgtcgttctg gtaaaggtcg gtgatccagc ccagggcctg cggcggcggg
     1141 gtgatgttgt cgcccttgga gcagaacacg atgatgggcg actggatgtt gcgcaggtcg
     1201 atgcggatgc cgtcggaggt caccagtccg gcggtggcga gccggttgcc gacaaagagg
     1261 ttgtcgacga tgtactggat ctctgggccg ttcaggtaca catggccgcc ccaccatttc
     1321 tcgaagccca ggtagcgctg ggtttcggtg tcgatgttgg cgtagaggtt gtactgcttg
     1381 tcccaaagcg tgttggccgg gttgaggctt tcgaagttct gcaccagcca ggcgccgtcg
     1441 aaggtgccgt tgcccaggtc gctggccagc gcggtcatcc agctgccgcc cagcaggccg
     1501 ccggaatagc gcatggggtt catgccgcgc cagccggccc agtaggacag cggcgcgccg
     1561 gcgatgatga tggggccgaa cagctcgggc cgcatggcgg ccgtcatcat gatctgccag
     1621 ccggcctggc agttggccac caccaccggc ttgccttcgg cctgggggtg cagggcaatg
     1681 atctcttcga gaaagtgcgc ttcggcgtgc atcacgtctt ccaccgtctg cgtggcggtg
     1741 gggcgcggca ggaagctggc gaagtagcag gggtggccgg cgcggatcgc cacgccgatc
     1801 tcgctttcgg gcttgaagcc gccgatgccc gggccgtggc cggcgcgcgg gtccaccacc
     1861 aggaaggggc gcttgatcgg gtcgatgacc acgccttcgg gcgggatgat gcgcagcagg
     1921 gcgtagttga cggggcgctc gaagccgcgc ccgtccagga cgacttccga attcatggac
     1981 agcacgttcg gcgccttctt ggcgatgtgc tggcggtact ggttgccgcg ttggcgcatc
     2041 acgtcggtga acagcacccc gcgctgccag gcgtcgacgc cgtattgcca ggcggccagc
     2101 cagggattga gcgtgggatc ggcgtagggg ggctggtccg gcgtggtggc ggaccgggaa
     2161 tcgggtgctg ccatggcagt ctcctgggga tgggcggtcg gcctgggcag gccgtctccg
     2221 ccggccggag ccgacgggtg tttaccctat tacaccgcaa cggatgttgc aacgcaacat
     2281 caaaaaatat tcatataaat caataggttt tatagggcag gaatttgccg ctcaggatga
     2341 tccgcacgcg gtcgcccttg gggtcggcct cgcgcgagat atccatcttg aagtcgatgg
     2401 cgctcatgat gccgtcgccg aactcttcgt ggatcagggc cttgaaggtg ctgccgtaca
     2461 cgctgaccag ctcgtagaaa cggtagatga gcgggtcggt gggaacggcc gtgggcagcg
     2521 agcccttgta gggcaccacc tgcagcagcg cgacggcctc cttgggcagc tcgagcagct
     2581 tgccggccgc cttggcctgc ttctcggtca gggtcatctg gccgagcagg gcggccgtgg
     2641 tccattcctt gctctggccg acggcgcggg ccaggtcttc ccatttgagc ttcttggcga
     2701 tcttctgggc gacgaccagt tcggtcacgt cgttgcgagt catcatggca aagcctccgg
     2761 gtgttgggag agggggaaac cggcggatca ggcctgcgcg atcccggcgg cctgcagcgc
     2821 cgcgcgggcc ggcgcgtcgc cgtccggcga gacggggcgc gtgaggtccc acaggaagcg
     2881 cacccgaaag cccgcttgcg cggcatcctg ggccgtccag agcacgcagt agtcgcgcgc
     2941 caggccgcag acatggactt cggcgatgcc gcgttcgcgc agccagccgg ccaggcccgt
     3001 ggcggggcgg atgccgccgg ggccgtggtt ttcacggaag gcgctgtagg agtccacctg
     3061 cgggttcgtg cccttgcgca ggatcaggtc gcaggcgctc cagtccacct ggccatgcag
     3121 cgcggcgccc ggcgtgcctt gcacgcaatg atcgggccac agggtttgcg gctggccgtg
     3181 cagggtgatg gcctggaacg gcgcggcgcc ggcatgccgg ctggcgaagg agacgtggtc
     3241 ggccggatgc cagtcctgcg tggccaccac ggtgcgatag cggcgcgcgc gcagcagggc
     3301 gtcgatgtcg gccacgatgg cgtcgccctc gtggcaggcc agcggaccgc ccggcatgaa
     3361 gtccggctgc aggtccacca cgatcagggc ggtgtcgtcg gcaaaggtgg acatgggcgg
     3421 actcctggcg cggctcaagc cgcgagtttc ttttgccaga acagggcgcg gccgtgctcg
     3481 tcctgcagct ggtagccctc gaagcccatc ttttgataga gcgtcctggc cgggatattg
     3541 ccttccagca cttccagcgt cagcttgcag cagcccatgg cgcgggcgcg gtcttcgagg
     3601 gcggcgagca acttctgcgc gacgccctgg cctcggaagc gttcggacac catgaagtcg
     3661 tgcagattca ggagcggctt gcaggcgaag gtggaaaagc cttccaggta gatggccagg
     3721 ccggccggct ggccgtcgac gcgggccgcc agcgcatggg cccaggggcg cgccgccagc
     3781 gaggcgacga ggttgccgcg gctgaattcg gacaggggct cgttgccgcc catgtcgccg
     3841 ctggcgtatt cgtcgagcag gccgaccacg atgcggccgt gttcggggtt cttgaaatcg
     3901 atgtcgatga tgtccagcat attgtgcgtg tcctgaggtg ttgcccgtgc cgccggcccg
     3961 cgggccaggc gaaacgcgct gccggccagt atagagagcc ccgcgcgggc ccggctattg
     4021 gcgccagccc ttatgattgc accatgaccc ccacgaccgc ttccgcttcg gcgcgtatcg
     4081 cgcgccgcca ccccgacgag ctcatcgtcg gcctggtgtc cgtttccgac cgcgcctcgg
     4141 ccggcgtcta cgccgacgag ggcgtgccgt ccctgcaggc atggctgggc acggcgctgg
     4201 cctcgccctg gcaggccgtc gagcggctga ttcccgatga cgcggccggc atcaccgccg
     4261 cgctcatcga cctggtcgac cattgcggct gcgatctggt gctgaccacg ggcggcaccg
     4321 gcccggcgcg gcgcgacgtc acgcccgagg ccacgctggc cgcgggcacg cgcgagatgc
     4381 cgggcttcgg cgagcagatg cgccagatca gcctgaagtt cgtgcccacg gccattcttt
     4441 cgcgccaggt ggcggtgatc cgcgaaaccg ccagccatgc ggcgctcatc atcaatctgc
     4501 cgggccagcc caaggccatc cgcgagacgc tcgaaggcct gcgcggcggc gacggcgccg
     4561 tgcaggtgcc cggcatcttc gccgccgtgc cgtattgcat cgacctgatc ggcggcccgt
     4621 atgccgagac ccggcccgag gtgatccagg ccttccggcc caagtcggcc cggcgcggcg
     4681 cctagccagg cccgacgtgc gcgatcgccg gcactggctg acccgcgagc gcatcgtgct
     4741 ttacggcacg ctgatgctgg tgtttttcct ggcggcggcc atcatgaacg tcaagacctc
     4801 ccgggatggg ttggtcgccg ggacccaccg gccgctgggc tcggatttca tcgcgttctg
     4861 gggggtctcg cacctggcgt tgtccggcca tgcggcatcg gcctacgact tcgacatgcg
     4921 ggccctgacg catgccgagc aggtcgccgt gcccgcatcc gacgtcagct atccctggaa
     4981 ttatccgccc gccttcgtcc tgctgattct gcacttggcc ctgatgcctt atttcgtggc
     5041 cttctgggta ttcatggcga ccacgctggc catgtacgtg ctggctttct ggcgcgccgt
     5101 ggggcaggga ctgggcctgt ggtgcctggc gatctttccc gccgcatgga tcaatttcgg
     5161 cggcggccag aacggctttc tgacggccgc gctggcgggc ggcgccttgc tgtgcctgcc
     5221 gcgccggccg gtcatgtccg gggtgttgat cgggctgctc tccatcaagc cgcacctggc
     5281 ggtgctgttt ccgctcgcgc tggtggccgc gggggcctgg cgcacgatcg ccgtcgcggc
     5341 ggctacgggt cttgccgtca atgcgctggg aacggccgtg ctgggcacgg atgtgctgtg
     5401 ggcgttctgg cgcggactgg ggcacgcgca gggcatgctg gccgccggcg agcgttttta
     5461 ctggaccaag ataccctcgg tcttcgtcat ggtgcgctcg ctggggggcg ccgcgtctct
     5521 cgcctatgcc gtgcatgccg cggcggcggc cgtggcggca tgggtggtct ggcgggtctg
     5581 gcgcgcaagc ggcgattgga tgctgcgcgg ggccgcgctg atgaccgcga cgtttttcat
     5641 cagcccctac gactacaact acgacctggt ctggctggcc ttccccatcg cctggctggc
     5701 gctggtggcc cagcgcgagg ggtggcgtgc gctggatcgg gaaatcctcc tggcggtcgc
     5761 gctgtggccc ttgctgtggt tcttcccgga ttgggcgttc aaggtgcagc tcagcccgat
     5821 cccgctggcc gggctgctgt gggtcatccg gcgccgcgtt tcctaggccg cctttttctg
     5881 ctcgaggtcg agctgggcgc agagcgcgcc gtacagccgc tcggcgtaat gcagggcgtg
     5941 ggtggtctgc tggtgcaggc gctgctgctc gtccaggcag gtgtcgtact tggagcgggc
     6001 ctggatgtag gtcagcccgg tattgcgcag gctgctgatg aagggcaccc gcgagcggcg
     6061 cagctggcgc aggccgcgct cgccccgctc gaacgcccgg cgcgccaggg ccaggcggtc
     6121 ggccgcctgg cgcagggcat cgctgggaga agtggggatc tttgccatgg cgaggattct
     6181 agccggcgcg gggcgggcgc cataggcccg gaatcggccg gaaattcccc tcaaatcgcc
     6241 gcgccggcgg ttctgccccg gccagcaacg tctgctcaca agatctttcc cgcttcgacc
     6301 ggtaaaatcg gcgctatatc gcggattcct ttcaccatcc attaaaaaac tgccccagga
     6361 gatccttcca tggccctcgt ttccatgcgc cagctgctcg accatgcggc cgaacacggc
     6421 tacggcattc ccgccttcaa cgttaacaac ctggaacaag tccaggccat catggaggcc
     6481 gccgccgaga ccgacagccc ggtgatcatg caggcctcgg ccggggcgcg caaatatgcg
     6541 ggcgagggct ttctcaagct gctcatccag gccgccgtcc agacgtatcc ccacatcccc
     6601 gtggtgatgc accaggatca cggccagtcg cccgacgtgt gccagggcgc catcaacctg
     6661 ggcttctcca gcgtgatgat ggacggctcc ctgatgtcgg acggcaagtc ggtcgccagc
     6721 tacgactaca acgtcgacgt cacccgcaag gtggtcgaca tggcccacaa gctgggcgtc
     6781 accgtcgaag gcgaactggg cgtgctgggc tcgctcgaaa ccatgaaggg cgacaaggaa
     6841 gacggccacg gcgccgaggg caccatgacc cgcgaacagc tgctgaccga cgtcgagcag
     6901 gccgccgact tcgtcaagca gacccagtgc gacgcgctgg cgatcgcgat cggcacctcg
     6961 cacggcgcct acaagttctc gaagaagccg acgggcgaca tcctgtcgat ccagcgcatc
     7021 aaggaaatcc acgcgcgcat ccccaacacc cacctggtga tgcacggctc gtcgtcggtg
     7081 ccgcaggagc tgctggccga gatccgccag ttcggcggcg acatgaagga aacctacggc
     7141 gtgccggtcg aggaaattca ggaaggcatc aagtacgggg tgcgcaagat caacatcgac
     7201 accgacatcc gcctggccat gaccgccgcc atccgccgtt tcttcgccga aaaccccgcc
     7261 aagttcgacc cgcgcgaata cctcaagccc gccaaggccg ccgccaaggc agtgtgcgtg
     7321 gcccgctacc agcagttcgg caccgccggc aacgccagca agatcaaggc cgtctccctg
     7381 gcggacatgg ccgcggacta cgcctgcggc aagctggccc aggtcgttca gtaaaccgac
     7441 gcgcaaggga tttaaaatcc cttgccacat tccggccccg agctcgttgt gagccggggc
     7501 ccgttctttc ccagataggc ctttccgtga ccaacgccct gcaccaatcc accatcaaat
     7561 ccctgccgct gctgggccgc ggcaaggtgc gcgacatgta cgccgtgggc gacgacaagc
     7621 tgctcatcgt cgcttccgac cgcatttccg ccttcgacgt gattctcgac gaccccatcc
     7681 cgggcaaggg ccaggtgctc accgaactca ccgaattctg gctgaagaag ctgggccacc
     7741 tcattcccac gcacgtcacc ggcatcaagc ccgaggacgt ggtcgcgccc gacgaagtcg
     7801 accaggtgcg cggccgcgcc atggtggtca agcgcttgaa gcccgtgctg gtcgaggcgg
     7861 tggcccgcgg ctacctgatc ggctcgggct ggaaggacta ccaggaaacc ggctcgatct
     7921 gcggcatccc gctgccggcc ggcctgaagc aggccggcaa gctgccccag cccatcttca
     7981 ccccggcggc caaggccgag ttcggcatgc acgacgaaaa cgtcgacttc gcccacgtgg
     8041 tcaaggaagt cggccaggaa atggccgaga agatccgcga catcacgatc aagctctacg
     8101 tcgaggccgc cgcgttcgcg gccaccaagg gcatcatgat cgccgacacc aagttcgagt
     8161 tcggcctgga cgagcagggc accctgtacc tgatggacga ggtgctcacc cccgattcgt
     8221 cgcgtttctg gccggccgac ggctaccgcg agggcatcag cccgccgtcg ttcgacaagc
     8281 agttcgtgcg cgactggctc gagacccagc cctggggcaa gaccccgccc gcgccgcgcc
     8341 tgcccaagga cgtgctcgag aagacggcgg ccaagtaccg cgaggcgctc gaccgtctga
     8401 cggcctgatc cgtccccggc ttgagtggca agaatagtca aggcatcccg gcgctcattt
     8461 agttgcaaca atttcgcttt gttacctata ctgcttgtgc ctaaatatgg gagagcagga
     8521 catggtgatg gtgttcccct ttttgaccgg agccttggcc atctgctacg ggatgctggg
     8581 caagcggcct gcctgcttcg tcctgtggct gatcacgctg ggtctattcg cgggctgggc
     8641 ctaccagcat ctcgacggcc cgttgccgct gtcgctgtga aggggtaggg gccggatggc
     8701 actctttctg caaaaccccg ggcacggctc gcgccagttc aacgccttgc tgctgttcct
     8761 gatctgcgcc agcctggtct tctcgctggt gtggcagggc ctggcggtgg actatgcctg
     8821 cccgatgtgc gccttgcagc gcgtggcgct ggtgctggcc ggcgtgggcc tgctgctcaa
     8881 cgtccgcctg ggcccttcgc ccttgcacta cgccatgacc atcgccgccg ccctggccgg
     8941 cgccgcgatc tccggctggc tggccctgcg gcccgccgac ctgggggccc ggctgctttt
     9001 cggcctgcac ttgtacagct gggccttcct ggtcttcggc gcgctggtcg tgttctcgct
     9061 cctgatgctg gcgctcgacc gccgctgggg cgacaacgcg ctcaagtgcc ccgtgtcgat
     9121 gcttggcgcc gtggtgatgg ccttgttcct ggcggcggcg ctggccagcg ccgcgggcac
     9181 cagcctcgac tgcggcgtgg ccgcctgtct caagcagctt gcctacgcgg cccgctagcc
     9241 cgcgcccgcc gcgccggcgt cagaccgtcg gcgaactcat ccgcggcggc gcgtccatcc
     9301 tgacctggaa cgccatccag cagacgcata cgctcaccag ccccagcgag ccgccgaacc
     9361 agcccacgta cgccacgctc acgtggttgg cgatgaggtt gcccagcagg gcgcccgtgc
     9421 cgatcccgat gttgtagatg cccgagaaca gcgacatggc caggtccgtg gcgtccgagg
     9481 ccagccgcag tatccaggcc tgcatggcca ggttgaagca gatgatcgcg gctccccaca
     9541 gcagggcgtg catcgacagg acggccgggc tggcggcgct gggccgcaag gtcagcaggc
     9601 aggcggtcag cacggccacc gtggccagca ggaagcgctt gggctgcctg gcgtagtggc
     9661 gattgaagaa catggccccc ggaatgcccg cgataccgaa gagcagcagc atggccgtga
     9721 cggcgttgtc gccgagcttg gccacctggt agacgaaggg ttcgatatac gtgtaggcgg
     9781 tgaagtgcgc cgagatggcg accgcggtca gcacatacag caggaccagc gtgggccggc
     9841 ggaacaggga cggcaagctc gcgagcgagc ctgcgttctg gctgggcagt tcgggcaggg
     9901 tcgcgcgcag gaccgccagg atgagcgagg cgacgacggc gatggccagg aaggtcgtgc
     9961 gccagcccag gacctcgccc accacgcgtc ccagcggaat gcccagcacc atggccatcg
    10021 aggtgccggt ggcgatgagg ccgagcgcca ggcccttgcg gccttcgggc gccatccgca
    10081 ccgccagggc cacggaaatc gaccagaaga tcgcgtgcgc gccggcgatg cccagccggc
    10141 ccagcagcag caccggaaag ctccaggcca cgcccgccag gacgtggctg gcgatgaaca
    10201 gcacgaacac cagggtcagc agcttgcggc gctcggcctg gccggtcagc agcatgccgg
    10261 gcagcgacag cagcgccacg gcccaggcgt agagggtcag catgaggccg acgtcgttgg
    10321 ccggcatgtc gaagctcgcg ccgatggcgt gcagcaggcc cacgggaacg aattcgctgg
    10381 tattgaaaat gaacgccgag aacgccaggg cgatcacgcc ccaccagcgg cgcttcggcg
    10441 acgaagcgga ggtcatttga gtttcttggg aaaaaaagag aaagcacgaa ccgtgcggac
    10501 gacgttgcgc gggtccgcgg gggtcaggtg tcggtcggga tcggcatgaa aatacccatc
    10561 aagggcgcgg gtccggggcc gggtatgaaa cgccggccgg aaagcggact ggccatgcaa
    10621 aaaattctag caccggggca aggccgcgca gcggcccgca agcgaaaaat gcggcgctgc
    10681 gccacggttt tggcgttttc gccacaaccg gcggttcagg cggccagcag gggccgcttg
    10741 gcgagttttt ccagcacgcg caccggcgcc agttccgcat agcccaatgc cgccagcggc
    10801 agctgccagg tctgctccag catgaactgt ccgcccatcg ccgcgtgcgg cacctggtcg
    10861 gagaagcaca cccaggtgct gcccggcatg aaatccacct gcgtccagtc gccctcgcgc
    10921 tggtagcgct cgtcgcgctt catggcgtcg tgcatctgca gcatcaggtg gtcgtactcg
    10981 gtgcggcgcc gcttggtgat gcccgccttg tgcatgagcc cggccagcca ggggcgccag
    11041 ggcttgttca tcccgggcag gtagtgccgc gccgatgcct cgaacgagcc gcccacgcgc
    11101 caggtgcgcg gctcgttgcg ggggttgatg ttggtgaaca cgcgcaggat gcgctcgccg
    11161 tgcagcggcc gcgagggaaa ggcatcgacg tgcagcagcc ggtcgtcctt gcgccacgaa
    11221 gggtgccagg tgccgatggc atgcaggcgc aggctggtgg ccggcgaatg gcggtggtcg
    11281 cgataaccgg ggaacagggc atcgagcagg gcgccggtgg aatccaggta gcgttgcagc
    11341 acgtcctgca cgcggtcgcg cgcggcgccg gtcgccacgg tgccggccag gcccttgggc
    11401 gtgaggctga tgttcttgcg cttggggtcg gtgagcgccg ggtcgagcag ggcctgttct
    11461 gcctccgtca gcgcgaagcc caggtgcggg aaatgcagca cctgcccttg ttccagcgcg
    11521 tgcagcgcct gcgccgacag ccccgcggcg tccggcgaac gccagtcccg caggggcagg
    11581 tcgatgaccg tggcggacat ggaggaggca gtcggcgagg cgggagcgct catggcgggt
    11641 aaaatccagg gttttccggc gggcggcccc gagagatggc gctgcccagg caattatccg
    11701 aactttactc catggctaag acagactcga cggcgcaaga cgccacgcag gaggccactc
    11761 cggtggtcgg cgtgatcatg ggctcctcca gcgattggga cgtgatgcag cacgcggtcg
    11821 ccatgctgcg cgacttcggc gtgccttgcg aggccaaggt catctcggcg caccgcatgc
    11881 cgcaggacat ggccgattat ggcgcgcagg ccgcaggccg cggcctgcgc gccatcatcg
    11941 ccggcgccgg cggcgcggcc cacctgcccg gcatgatggc ggcgctgacc gaggtgccgg
    12001 tgttcggcgt gccggtgccg tccaaatacc tgcgcggcga agactcgctg ctgtccatcg
    12061 tgcagatgcc caagggcatc ccggtggcca ccttcgccat cggcgaagcc ggcgcggcca
    12121 atgccgccct gcacgtcatc gccaacctgg ccaccaccga cgatgccttg cgccgcaggc
    12181 tggcggcctt tcgcgcgagc cagacgcagg cggcgcgcgc catgacgctg cccggggcct
    12241 gaatcatgcg tgatgtcgaa cccatcgcgc ccggcggctg gctgggcctt ctgggcggcg
    12301 gccagctggg ccgcatgttc tgccacgccg cgcagagcct gggctacaag gtcgccgtgc
    12361 tggacccggc cgccgacggc cccgcggcca tggtggccga ccgccacatc caggccgcct
    12421 atgacgatgc cgccggcctg gccgcattgg ccgaactctg cccggccgtg accaccgaat
    12481 tcgagaacgt gccggccgac agcctgcgcg cgctgtcgac gcgctgccgc gtcagcccgg
    12541 cggccgacgc cgtggccgtc gtgcaggacc gcatcgccga aaaaaccttc atcgcctcgc
    12601 aaggcatccc ggtggcgccg catgccgccg tgcgcggcga ggccgacctg cgggccgcgc
    12661 ccgacgcgct ctttcccggc atcctcaagg tggcgcggct gggctacgac ggcaagggcc
    12721 aggcgcgcgt gcagacccgc gccgaggcgc tcgccgcttt cgccgcattc cagggcgtgc
    12781 cctgcgtgct cgaggccctg ctgccgctcg actatgaaat ctcggtcgtg ctggcgcgcg
    12841 gcttcgacgg cgccgacgtg gcctatcccg tgtcgcgcaa tgcgcatcgc gacggcatcc
    12901 tggcggtctc cacggtggcc ggcgacgact gtccttcccc gcatcgcgag gccgccacgc
    12961 gggcggcgcg cgccatcgcg cagggcctgg gctaccacgg cgtgctatgc gtggagttct
    13021 tcgtgctcgc cgacggccgc ctgctggtca acgagatcgc gccgcggccg cacaacagcg
    13081 gccactacag catcgatgcc tgcgtcacca gccagttcga gcagcaggcc cgcgtcatgg
    13141 cctgcctgcc cctgggcagc acgcgcgtgg tggcgccttc ggtcatgctc aacatcctgg
    13201 gcgacgtctg gtttcccgcc ggcgccggga cggcgcgcga gcccgactgg ccggccgcgc
    13261 tggccgcctc gtccagcgcc aagctgcacc tgtatggcaa gcgcgaggcg cgccggggcc
    13321 gcaagatggg ccacatcacc atcgtgggcg agacgctggc ccaggcccag gccgatgcgg
    13381 cccgcgtggc cgcggcgctg ggcatggccg ccccgcaatg acgcggatga acgccccggc
    13441 cccgcagccc tccaggcatg acatcgagca ggcggcccgc cgcctgttcg cgggccagct
    13501 ggtggccttt cccaccgaga ccgtctacgg cctgggcggg gacgccgaga acccgcaggc
    13561 cgtggcgcgc atctacgccg ccaagggccg tccggccaac catccggtca tcgtgcacct
    13621 ggcgcccgag ggcgatccgg cctattgggc cgcgcaaata ccgcccgagg cgcgcaagct
    13681 catgcaggcc ttctggcccg ggccgctcac gctgatactc aagcgcgcgc cgcacattcc
    13741 cgacgccgtc agcggcgggc aggacagcgt cggcctgcgc tgcccctcgc accccgtcgc
    13801 gcaggccctg ctgcgggcgt tcgcggcgct caaggccagc ggccagggcg gcgtggccgc
    13861 gccgtcggcg aacaagttcg ggcaggtctc gcccacgctg gccgcgcatg tgcgcggcga
    13921 gtttcccgac gaggtggcgc agggcatgcc cgtgctcgac ggcggcgcgg ccgaggtcgg
    13981 catcgagtcc accatcctcg acctgtcgcg cctggaccag ggcgtgggcg ccgtgctgct
    14041 gcgccccggc catatcagcg ccgcgcagat cgcggccgtg ctgggccagc cggtgcatcg
    14101 tcccgacgcc gccgcgccgc gcgcctcggg cacgctgaaa gcccactatg cgccgcatac
    14161 cccgctggaa ctgatcgacg acgcgcgcct ggcgcgggtc gtgcaaggcg cgggcctgcc
    14221 cgcgggccgt tgcgtgctgg tgagctatgc cgagctgccg ccgctgccgg ccgatctggc
    14281 ggcgcgcgtg caggtgcgtg ccgtgccggc cgatccggcg cattacgcgc aggcgctcta
    14341 cgccaccttg cgcgagctcg acggcggcgg ctatgcccgc ctgctggtgc aggcgccgcc
    14401 gcgggcgccg gcgtgggacg cggtcaacga ccgcatcggc cgggcggccg cggcattcac
    14461 gctcgatacc gcaggccttt gaggcgcctt gcgcctcttg cgctcaggcc tggaaatagg
    14521 ccggctgcgg gccggtgacg acggcccggt attcctcgat cagcagctcc gcgtggcgcg
    14581 tcaacggccg cgcgcgggag cggatgatgc gcgaatacat gcacagcgtg ggccggaacg
    14641 gccgtatcac gaaggcatcg agcggaaacg gcacgatggc ggcgggttcg accaggccga
    14701 cgccgacgcc cgcgcgtatc aaggcaagca gcgcggccga cgaagtggcc tcgacggcca
    14761 tcggcatgtg gatgccggcc tcggtgaagg cgctttccac cagatagccg aaattggtct
    14821 gcgggccgta ggacaggaaa gtctccggct tgagatcctg cggcgtgacg cagtccagcg
    14881 actcgaagcg gtggccgcgc ggcgcggcgc acaccacgtg ctgcaggcca atgtcttcgt
    14941 tgtgcgtgtc gggcgacggc gtgatgtcgt gcaccaggcc gaaatccacg cggccgtttt
    15001 gcacgaggtc gacgatgggc ggattggcca ggatctcgac gctcggggtg atgccgggaa
    15061 agcgctggcg cagccgccgg atggcttcgg gcaggaaggt cgtggcctgc gtgggaatgg
    15121 ccgccacggt gacggtgccg ttatcggggt cggccaggcc gcctatggcg cgctggacgg
    15181 cgccgtaggc atcgcgcagg cgaccgacgc tggcctgcag ctggtgggcc tgctgcgtgg
    15241 gcgtcaggcg ctggtgcgcg cgttcgaaca ggcgcaggcc ggtgtcctgc tcgagctgct
    15301 gcagcatgcg gctggcgccc gactgcgtca tgcccaggag tttggcggcc cgcgataccg
    15361 agcccgtgac catgacggcg ttgaagacgc tcaactgggc aaagctgggt tggtgcagcg
    15421 tggtcgggcg tgtcaggccc agggcggggc gtgggctcat gcagtgtcgg cgcagggccg
    15481 ccaggtgaac gttgcggtgg aaaggccgtt taggtggaaa tacggatagg tgtaaaagtc
    15541 atatcttgca tgaataatcg ctggttcgtc attatgggtc tcccttaccc ccgcactgga
    15601 gccccgtcat gagcaaaccc gtggcagatc cctgcctgct ggacgcagca caacgcgcct
    15661 tgtccgatcc cggactgggc tccgaacggc gccggcagtt gctgggcatg ctgggcgcgg
    15721 cgggcctgac ggctttgctc cccgccggtc aggcccaggc cgatgccggc aagatcgtgg
    15781 tgtgcaactg gggcggcgat gcgctcaagt accagccgca ggcctggggc cagcctttcg
    15841 agaaagcctc gggcatgaag gtcgaattcg atggcaccgg ccccaccttc ggcaagatcc
    15901 gcaccatggt tgaatcgaac cacgtggtgt gggacgtctg cgatgccaac ctggccgtgg
    15961 cgcattcgct gggcaagcag ggcctgctcg aacccatcga ctaccgcatc gtcgaccgca
    16021 acaaggtgcg gccgggcttc agcagcgaat acggcgtgtc gcacttcatc tacagcttcg
    16081 tgctggcgtt cagcaagaag gccttcccca agaaggcccc caacacctgg gccgatttct
    16141 ggaacgtcaa ggacttcccg ggcacccgca tcctgcccga ccgcatcctg gcttcgctgg
    16201 aagccgcgct catgagcgat ggcgtgccgg ccgatgcctc caagatctat cccatcgacg
    16261 aaaaacgcgc gctgtcgcgc atcgccgcca tcaaggacaa cgccatcttc tggaagacgg
    16321 cggccgatag ccagcagctg ctgcgttcgg gcgaggccgt catggggctc atctggcata
    16381 cgcgggccgt gctgctgcgc cgcgagcccg ggtcggacat cgacttcacc tggaaccagg
    16441 gcatactcat cccagccggc tggctggtgc ccaagaacaa tccggcgggc cgcaaggtgt
    16501 tcgacttcat cgcctccacg cagtcgcccg aaagccagat cgaactgctc aagctgatgg
    16561 gcaacggccc ggccaacccg gcggccagcg ccatggtgcc gcccgaactg cgcaaggaca
    16621 atcccaccga tgacgacaac ttccgcctgc aggtcgtggc ggactcgacc tggcacggcg
    16681 agaaccagga ccgcgtctac aacgattacg tcaaggtcat cacaggctga tcagggcgcg
    16741 ccgatgtctt ttcacgcaga tctcgttttg catggcgggc gcatcgcgac gctggacccc
    16801 gtcctgggcg tggtgcaggc cgtggccatc cgcgcgggcc gcatcgtggg ggcgggcagc
    16861 gatgccgact gcctcaacct ggccgggccc ggcacgatca aggccgacct gcgcggcgct
    16921 ttcgcgatgc cgggcatcca tgacagccac tgccatcccg acggccaggc ggtcaagcag
    16981 gggcgctggg aagacctggg cgaactgtct tcggccgagg ctttgcggca gcgcattgcc
    17041 gatttccatg cccgcatgcc ggccggcgcc tggtgcctgg gattccgctt cgatgaaacc
    17101 cgcatcggcg gctaccccgg ccgcgccatg ctggatgcgg ccgcgccggg ccgtcccgtg
    17161 ttcctgttgc ggcgcgatgc ccaggtgggc ctggccaacg ccgccgcctt cgggctgctg
    17221 cgcgatagcg gcctggcgga ccgagtgccc ggccattgcc tggagccggc cggcggcatg
    17281 gcgcgcggcc gcggggtctt cgcgttcacc acgctgatcg ccgcttccga tcgcatcgaa
    17341 gactatctcg acggctaccc cgtcgttttt cgcgagatgg cgcgcctggg catcaccagc
    17401 gtgcacaacg ccctgaccgg ccggctggcc gtgcaggctt atcgccggct caatgccggc
    17461 cgggccttgc cggtgcgcct gggcatgatg ctcaacggac gcgaccagca attggtcgac
    17521 gaggtgctgg aggccggcct gcgtttcggc gatggcgacg aaacgctgcg cctgctgggc
    17581 gtggaatacg gctcggacgg cagcaccagc gggcgcacgg cggcctacta tgcgccctat
    17641 gcgggccagg atggcggcga cggccagccg cccaaccgcg gcgacgtgaa ctacgaggcg
    17701 gatgatctcg ccgccaaggt ggcgcgcatc atggcggcgg gcctgcaggt cgcggccacg
    17761 ggcaacggcg accgcggcat cgacttcgcg ctggatgcct tcgaaaaagg actggtggcc
    17821 aacccgcgcg ccgtgccgcc gcgcatcgag cattgctgct gcgcgccggc cgccacccag
    17881 gcgcgcatgg cgcggctggg cgtgatcgat tcgtcggcca cgggctttct ctacagcctg
    17941 ggcgatgcct acctgcgcaa ccggcccgag ccggacatgg cctggctgtg gccgcaccgc
    18001 gacatgatgc gccggggcgt gctggtctgc ggccattccg acgcgccggt gtgcgagcgc
    18061 aatcctttcc tgggcatctg gtccatggtc acgcgcaaga cggcgtccgg cgccgtcatc
    18121 ggcgcgcatc aggccctgtc gatagacgag gccttgcgct gctatacggt caacccggca
    18181 agggccgagg gcctggcggc cggcaaggga accctgtcgc ccggcaagct ggccgatctc
    18241 atcgtcctcg accgcgaccc gtacgccatc gaggccgacg agctgcggca cgtttgcgtg
    18301 cgggccacgc tgctcggcgg caaggccgtg tatcgcgacg gcggcctgga cgccgccctg
    18361 cccgaggccg cgtggtgagc cggccggcgc tgcgggccag ccaggccgtg ctccccggct
    18421 tgctggtcct gcccgtggtg gtgctggtgg gcggcctgtt cctggcgccg gtgctgcgcg
    18481 tgctgtcgct cagcttcatc gagcccgtgc cggggctggg caactatgcc catctctttg
    18541 ccgaacccct gtacctgcgg atcatggcga cgaccttgcg cgtctgcctg tggacgaccg
    18601 cgctgtcgct gctgctggcc tacgtcctgg cctatgtgat ggccaacgtc ggcgaacgcg
    18661 cgcgcagctg gatgctgatc ggcctgctgg tgccgttctg gctttcggtg ctggtgcggg
    18721 ccttcgcctg gaccatgctg ctgtcgcgca cgggcgtggt caacgccacg ctcatggccc
    18781 tgggcgtgat cgacagcccc ctgctgttgc tgcgcaatga aacgggcgtg ctgatcggca
    18841 tggtgcatta catggtgccg tacgccgtgc tgccgatgct gacggccatg ctgggcatag
    18901 acagggcgct gacggcggcc gcccgcgccc tgggcgcggg gccggtccgg acattcgtct
    18961 cggtgtatct gcccttgtcg atgcccgggg tgctggcggc cgccgtgctg gtcttcatct
    19021 tcagcatggg tttcttcatc acgccggcct tgctgggcgg cggcaaggtc atgatggtgg
    19081 cccagtacat cgagttcggc atcagcgaga gcacgaactg gggcattgcg acggccttcg
    19141 ccagcgtgct gctgggggcg gtgatctgca gcctgctgct ggtgtcgcgc ttcgtgcgta
    19201 ttccttccct gttcggcgcg gcatcatgac ggtgcggcct tcatggtggt tcaggctggc
    19261 ccaggtcctg gcattgctgg tgctggccta cctgctgctg cccgggctgg cggtgatccc
    19321 ggtttcgttc accgaccgca gctacctgtc ctttcccgcg cagcactggt ccttgcagta
    19381 ttaccggcaa ctgatcgaca gcccggcctg gcgcgacagc gcgctgcaga gcatcatggt
    19441 ggcggccata tcgacggcgc tggccacctt gctgggcagc ctggccgcca tcggcgcctg
    19501 gcgcctgggc ggacgcctgg gcggcctggt gcggctgctg gcgctggtgc ccctgatcgt
    19561 gccgaccatc gtgggcgccc tggcctatta ccggctctat atcgacctgg ggctgctgga
    19621 cacgtttgcc ggcgtggtgc tgacacacgt gatcgccgcc atgccgttcg tgttcatgtc
    19681 ggtgggttcg gcgctgtcga acttcgatcc gcggctcgaa caggcggccc gctccatggg
    19741 ggcccggccc tggcaggtcc tgcggcgcgt cacgctgccc gcgatccgtc ccggcgtgtg
    19801 gtcgggcgcc ttgttcgcct tcatcgtctc ctgggacgaa atcgtggtgc tgctgttcgt
    19861 caccacgcgc gatgtctatc tgctgccccg cgccatgtgg gacggcatca accagaacgt
    19921 cgatccgtcg attgccgccg tcgcgacctt gatgatcgcc gtcacctttg cctgtttgtg
    19981 cgtccagcgc ctgctcgtgg gacgccgggt tcgccgggag tcgccatgag ccagttttcg
    20041 gcgccggatc gcgccttgtt gcgcgtgcag ggcctgcaca aatcattcgg cgcggtgaag
    20101 gcgctcgacg gcgtcgattt ttccctggcg cccggcgagt tcctgacgct gctcgggccg
    20161 tccggatcgg gcaagacgac cacgctcatg acgatcgcgg gcttcgtcga accgaccggg
    20221 ggccgggtgc tgttcgagga gcgcgacatc acgcgccgcc tgcccgagga acgggatttc
    20281 ggcatggtgt tccagggcta tgccctgttt ccccacatga cggtcgagca gaacatccgg
    20341 ttcccgctcg ccatccgcgg cgtgccgcgc ggccaggccg accggcgcgt gcgccaggcg
    20401 ctggagcagg tgcagctggg agcttatgcc aagcgccatc cgcggcaatt gtcgggcggc
    20461 cagcagcagc gcgtggcgct
//
LOCUS       DASWPO010000138         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2887033, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000138 DASWPO010000000
VERSION     DASWPO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887033"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGC69_07745"
     CDS             <1..439
                     /locus_tag="VGC69_07745"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEY0295212.1"
                     /translation="MQANGLPNTFVPGRNLLFLTLAAALGYRRQLEVLVGGMCETDFS
                     GYPDCRDDTIKSQQVTLGLGLGSRVVIETPLMWRDKAQTWALAHELGGQALVDLIVEH
                     SHTCYLGERGVRHDCGYGCGKCPACALRAAGWLRWQGASCSSS"
     gene            complement(865..1404)
                     /locus_tag="VGC69_07750"
     CDS             complement(865..1404)
                     /locus_tag="VGC69_07750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0295213.1"
                     /translation="MTTASPLIRDALDPDVPAIQRIYARHVLHGTASFELEPPTIEQM
                     RERRAHVAAHGMPYLVAEVDGVVRGYAYVTPYRPRPAYRHTVEDSVYVEEGWAGHGIG
                     GKLMAELIARCTAGGWRQMLAVVGDSANAASLALHARFGFRPVGTLRSVGFKHDAWRD
                     TVLMQRELGEAEHTPPARA"
     gene            1566..2489
                     /locus_tag="VGC69_07755"
     CDS             1566..2489
                     /locus_tag="VGC69_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295214.1"
                     /translation="MRGLNLDHLRIFLDVIEHGSFSAAAERHGLSQPAVSQQVRQLER
                     RYGLRLAERVGRRVTATAAGQALLDYARRIDAVAAEADRAMQSHAAGVGGRVLLGTGG
                     TACTYLLPPLLATLRGRFPDLDVVVRTGNTADVLRGIEDNVLDIGLVTLPAAGRMFQV
                     TPVLDDDFVAIFPAGEAGLPARATPAELARRPLVLFEPGARTRRLCDDWFEAAGVPAR
                     PVMELGSTEAMKEIVAAGLGCAVLPGLAVTGSGARASLAVRPLRPRLRRQLAVVLRHD
                     KPVGRGLRQVVELLQGLRGQPGRGAGAGRPR"
     gene            2701..>3348
                     /locus_tag="VGC69_07760"
     CDS             2701..>3348
                     /locus_tag="VGC69_07760"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0295215.1"
                     /translation="MPHYRSRTSTHGRNMAGARALWRATGMQDGDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGALVAGEIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKITSPTDGK
                     VVKLDLIDAMIKAADPKVTDAEAEMVERSACPTCGSCSGMFTANSMNCLTEAIGLA"
BASE COUNT          523 a         1143 c         1172 g          510 t
ORIGIN      
        1 gatgcaggcc aacggattgc ccaacacttt cgtgccgggc cgcaatctgc tgttcctgac
       61 cctggccgcg gcgctgggct atcgccgcca gctggaggtg ctggtgggcg gcatgtgcga
      121 gaccgatttc tcgggctatc cggactgtcg cgacgacacc atcaagtcgc agcaggtgac
      181 gctgggcctg ggcctgggct cgcgcgtggt gatcgagacg ccgctgatgt ggcgcgacaa
      241 ggcgcagacc tgggcgctgg cgcacgaact gggcgggcag gcgctggtgg acctgatcgt
      301 cgagcatagc catacctgtt atctgggcga acggggcgtt cgccatgatt gtgggtatgg
      361 gtgtggaaag tgcccggctt gcgcgttgcg ggcggctggg tggctgaggt ggcagggggc
      421 gtcgtgttcg agttcttgag ccgtctgtcg ggaggcgggc agggcgtgtc ggccacggcg
      481 gctgactcgg cccctggacg gggcctcggc catcgccttc ctcgtccggg cgggccgggg
      541 gcgaactcgc tgcgctgcgc tccgctcaaa cacgcccccg gaaggccccc gccctcccta
      601 cggaagcgct cggcggccgc ctaccggccg acacgccctg cccgcctccc gacagactgg
      661 acagcctcga aattcgagac gcttcaagca tcggtgcaag gggtatagcc attcggctgc
      721 gttgagcggc gggaagtccg aaggaccgag ggcgcctgat ggcgcgcccg agccgccgcg
      781 atgcagccga atggctatac cccttgcacc gatgcgactc aagaactcaa taacgcatcc
      841 aacagacacc catcacccag cccatcaagc ccgcgcaggc ggcgtatgtt cagcttcgcc
      901 cagttcgcgc tgcatcagca ccgtatcgcg ccatgcatcg tgcttgaacc ccaccgaccg
      961 cagcgtcccc accggccgga agccgaaccg cgcatgcaag gccaacgagg ccgcgttggc
     1021 gctgtcgccc accaccgcca gcatctgccg ccacccgccc gccgtgcagc gcgcgatcag
     1081 ctcggccatg agtttgccgc cgatgccgtg tccggcccag ccttcttcca catagaccga
     1141 atcctccacc gtgtgccggt aggccgggcg cgggcggtaa ggcgtgacat aggcatagcc
     1201 gcgcaccacc ccgtcgacct cggccaccag atagggcatg ccgtgcgccg ccacatgggc
     1261 gcggcgctcg cgcatctgtt cgatggtggg cggctccagc tcgaacgagg cggtgccgtg
     1321 caggacgtgg cgggcgtaga tccgctggat ggcgggaacg tcgggatcga gggcgtcgcg
     1381 gatgaggggg gaagcagtgg tcatgtcggc aagcggggag aagatggagc ccggaaaatc
     1441 cgcatagtgt gccttccctc catcaataag agaagatttg gacgcttttg cttatgataa
     1501 ggaaaagttt gacttgaaac cttggactcg aaacggccgc ggccctgcgc cgcgcctgcc
     1561 cggacatgcg cggcctcaac ctggaccacc tgcggatctt cctcgacgtg atcgagcacg
     1621 gcagcttttc ggccgccgcc gaacggcatg gcctgtcgca gccggcggtc agccagcagg
     1681 tgcgacagct cgaaaggcgc tacggcctgc gtctggccga gcgggtaggg cggcgcgtga
     1741 cggccaccgc cgcggggcag gcgctgctgg actatgcgcg gcgcatcgac gccgtggcgg
     1801 cggaggccga ccgggccatg cagtcgcacg ccgccggcgt gggcggccgc gtgctgctgg
     1861 gcaccggcgg cacggcatgc acctatctgc tgccgccgtt attggccacg ctgcgcggtc
     1921 gcttccctga cctggacgtg gtggtgcgca ccggcaatac cgccgacgtg ctgcgcggca
     1981 tcgaggacaa cgtgctggac atcggcctgg tcaccctgcc cgccgcgggg cgcatgttcc
     2041 aggtgacgcc ggtgctggat gatgatttcg tggccatctt tccggcgggc gaagccggcc
     2101 ttccggcgcg cgccacgcca gccgagctgg cgcgccgtcc gctggtgctg ttcgaaccgg
     2161 gcgcgcgcac ccggcgtttg tgcgacgact ggttcgaggc ggcgggcgtg ccggcccggc
     2221 cggtcatgga gctgggcagc accgaggcca tgaaggagat cgtggcggcc gggctgggct
     2281 gcgccgtgct gccgggcctg gcggtgacgg ggagcggcgc ccgggcgagc ctggccgtgc
     2341 ggccgctgcg gcccaggctg aggcgccagc tggccgtggt gctgcggcac gacaagccgg
     2401 tgggccgggg gctgaggcag gtcgtcgagc tgttgcaggg cttgcgcggc cagccagggc
     2461 gaggcgcggg cgccggcagg ccccggtagg tccctggacg cctggccggg agcccggtaa
     2521 atccggcgta ttattgaacc cctacggtgc cccgccggga aaacggctgc ggccgggcca
     2581 cctgtcgcga tgcccgcgca cctgtcacaa gcggtgccgc accgcgcgct ccctccgatt
     2641 tcgcattcag cacaacaatt gcccaggaca taccggggca tcgttccgac gagacaatcc
     2701 atgccgcact accgttcccg cacttccacc cacggccgca acatggccgg cgcgcgcgcc
     2761 ctctggcgcg ccaccggcat gcaagacggc gacttcggca aacccatcat cgccgtcgtg
     2821 aactcgttca cgcagttcgt gcccggccac gtgcacctca aagacctggg cgccctggtc
     2881 gccggggaaa tcgaggccgc cggcggcgtc gccaaggaat tcaacaccat cgccgtggac
     2941 gacggcatcg ccatgggcca cggcggcatg ctgtattcgc tgccctcgcg cgagctgatc
     3001 gccgactcgg tggagtacat ggtcaacgcg cactgcgccg acgccatggt gtgcatctcc
     3061 aactgcgaca agatcacccc gggcatgctg atggccgcca tgcgcctgaa catccccgtg
     3121 gtgttcgtct cgggcgggcc gatggaagcg ggcaagatca cctcgcccac cgacggcaag
     3181 gtggtcaagc tcgacctgat cgacgccatg atcaaggcgg ccgaccccaa ggtgaccgac
     3241 gccgaggccg agatggtcga gcgcagcgcc tgccccacct gcggctcgtg ctcgggcatg
     3301 ttcacggcca actccatgaa ctgcctgacc gaggccatcg gcctggcg
//
LOCUS       DASWPO010000139         5854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2976067, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000139 DASWPO010000000
VERSION     DASWPO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976067"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VGC69_07765"
     CDS             <1..926
                     /locus_tag="VGC69_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0295216.1"
                     /translation="DLNAVNEPSSPAIFAGVEHLSGPQYADYLAFIAKGHELPVACDC
                     KVEAVGAVPGGGFILDTSKGKLQAGFLIWATGEFQFPDLAPFPGAQWCPHYAQVVDWQ
                     AFKARRYTVVGGYESGVDAAFNLVKLGRKVRLLARKTTWDPHGPHDPSLSLSPYSRQR
                     LYETIDTGLLEIVFGVDVIGITQNAGGGFRIHAGDGRYWDEKQAPILGTGFLQGGGAQ
                     QIAELWDWDEDGRVVLSEADESTITPGLFLVGPQVRHDTRIYCFIYKFRQRFGLIAEG
                     IAQCLDRDASLLKTGAWGPFGNSECCEGCEC"
     gene            920..2008
                     /locus_tag="VGC69_07770"
     CDS             920..2008
                     /locus_tag="VGC69_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HEY0295217.1"
                     /translation="MLTALRNTFHSSWISLLLIGSIVAGAGIGHQWPTVGELLGGQVD
                     RTLLVLVGLLFFGVPFGAVAQLRGGVRVLGLILLANYLGVPAIGYAIASVLLPSQPLF
                     MVGLVIYFMSPCTDWFLGFTRLARGNFALGTALIPINMVVQLLLYPVYLHLFTRSPVE
                     VEAAIIGDTLLHWFLVPLAAAVLAHYGLHWLLGKARFSRVLHRVDQAAPWVIALLVME
                     IFAANIAVILEHRTVFAWVLLGVFLFFVSTFLLGEGISRLGRLAYPDHALLTMNIAAR
                     NAPLMLAVTMAALPGQPLIYAALVIGMLVEFPHLTVLQRILLGQRQFPGWSLRPSAEH
                     PGSTRTKKGNLHHDHQEQDRARCQRPFG"
     gene            2036..2194
                     /locus_tag="VGC69_07775"
     CDS             2036..2194
                     /locus_tag="VGC69_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY0295218.1"
                     /translation="MTTDAATGDVLMLGYMNAEALCRTIEAGEAHYWSRSLSGCECGS
                     PVPAQAAM"
     gene            2287..2873
                     /locus_tag="VGC69_07780"
                     /pseudo
     CDS             2287..2873
                     /locus_tag="VGC69_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299244.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2884..3120
                     /locus_tag="VGC69_07785"
                     /pseudo
     CDS             2884..3120
                     /locus_tag="VGC69_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
     gene            complement(3259..3612)
                     /locus_tag="VGC69_07790"
     CDS             complement(3259..3612)
                     /locus_tag="VGC69_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295219.1"
                     /translation="MSTCNHPQHTNHAHQHGPNCGHIAIRHEGHVDYLHDGHLHHPHG
                     DHIDEHVIAVSATNPDVCTPAHACAGHDQGHVHGPECGHQAVPHGDHVDYLVDGHLHH
                     PHGDHCDNHGPIALA"
     gene            complement(3642..3998)
                     /locus_tag="VGC69_07795"
     CDS             complement(3642..3998)
                     /locus_tag="VGC69_07795"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0295220.1"
                     /translation="MPHVHPTACQHDVDTVSAIDYGPGQLDTAAAMCQALSDPARLRL
                     LLWLAQREMCVSELVEFEQAKVSSISARLQTLHAARLVTRRREAKHIFYALADEHVHA
                     LLRNILGHAAEAAPGQ"
     gene            4195..4575
                     /locus_tag="VGC69_07800"
     CDS             4195..4575
                     /locus_tag="VGC69_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0295221.1"
                     /translation="MPPTWTISELSREFEITPRTIRFYEDQGIVSPGREGRNRVYDAR
                     DRTRLKLALRGKRLGLQLAEIRALIDMYDTPGDTELQLREYLNVLARHRSALEQQRLD
                     IQETLQEISEQERLCQELLLVKQA"
     gene            4608..5786
                     /locus_tag="VGC69_07805"
     CDS             4608..5786
                     /locus_tag="VGC69_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY0295222.1"
                     /translation="MNIPGLNFDLGEDVDMLREAVAAFAAAEIAPRAAETDRTDQFPM
                     DLWRKFGDLGVLGMTVSEEYGGTNLGYLAHMVAMEEISRASASVGLSYGAHSNLCVNQ
                     IHRNGNEAQKRKYLPKLVSGEHVGALAMSEPGAGSDVVSMRLRADKKGDRYVLNGSKM
                     WITNGPDADTLVVYAKTDPEAHQRGITAFLIEKGFAGFSIAQKLDKLGMRGSHTGELV
                     FQDCEVPEENVLGQLNGGVRVLMSGLDYERAVLSGGPLGIMQAVMDVAVPYIHDRKQF
                     GQSIGEFQLMQGKIADMYTVLQASRAFAYTVGKNLDRLGKEHVRQVRKDCASLILYTA
                     EKATWMAGEGVQILGGNGYINDYPTGRLWRDAKLYEIGAGTSEIRRMLIGRELFNETV
                     "
BASE COUNT         1013 a         1871 c         1906 g         1064 t
ORIGIN      
        1 ccgacctgaa cgcggtcaat gagccgagtt cgcccgcaat ttttgccggg gtcgagcatc
       61 tcagcggccc gcagtacgcc gattacctgg ccttcatcgc caaggggcat gaactgcccg
      121 tcgcctgcga ctgcaaggtc gaagcggtcg gggcggtgcc gggcggcggc ttcatcctgg
      181 atacgtccaa gggcaagctg caggcgggct ttctgatctg ggctaccggc gaattccagt
      241 tccccgacct ggcgcctttc cccggtgcgc aatggtgccc gcattatgcc caggtcgtgg
      301 actggcaggc gttcaaggcc cgccgctaca ccgtggtcgg cggctacgaa agcggcgtgg
      361 acgcggcatt caatctggtc aagctcgggc gcaaggtgcg gctgctcgcg cgcaagacca
      421 cctgggaccc tcacggtccg cacgacccga gcttgtcgct ctcgccctac agccggcagc
      481 ggctgtacga gaccatcgac acgggactgc tggagatcgt attcggcgtg gatgtgatcg
      541 gcattaccca gaacgctggc ggaggctttc gcatccacgc gggcgacggc cgttactggg
      601 acgagaagca ggccccaatt ctgggtacgg gatttctcca gggcggcggt gcgcagcaga
      661 tcgccgaact ctgggattgg gacgaagacg gccgcgtggt gctgtccgaa gccgacgaat
      721 cgacgattac gccgggcctg ttcctggtcg gcccgcaggt gcgccatgac acgcgcatct
      781 actgtttcat ctacaagttc cgccagcgct tcggcctgat cgccgaaggc atcgcgcaat
      841 gcctggatcg ggacgcgagc ctgctaaaaa ccggcgcctg gggtccgttc ggcaacagcg
      901 aatgctgcga gggctgcgaa tgctgactgc cctcaggaac acctttcatt cgtcctggat
      961 ctccctgctg ctgatcggca gcatcgtggc cggcgccggt atcggccacc agtggcccac
     1021 agtaggcgag ctgctgggtg gccaggtcga tcgtaccttg ctggtgttgg tcggcctgct
     1081 ctttttcggc gtgcccttcg gggctgtcgc gcaactgcgc ggcggcgtcc gcgtcctggg
     1141 gctgatcctg ctcgccaact acctcggcgt tccggccatc ggctacgcca tcgcctcggt
     1201 cctgctgccc tcgcagccct tgttcatggt cgggctggtg atctatttca tgtcgccctg
     1261 cacggactgg ttcctgggtt tcacgcggct ggcccgcggc aacttcgccc tgggcacggc
     1321 gctgattccc atcaacatgg tggtgcagct cctgttgtac ccggtctacc tgcacctgtt
     1381 cacccgcagc ccggtcgagg tcgaggcggc catcatcggc gatacgctgc tgcactggtt
     1441 tctcgtgccg ttggccgcgg ccgtcctggc ccattacggc ctgcactggc tgctgggcaa
     1501 ggcgcgcttt agccgcgtgc tgcaccgcgt ggaccaggcc gcgccctggg tgattgcgct
     1561 gctggtgatg gaaattttcg ccgccaatat cgcggtcatc ctggagcacc gcaccgtctt
     1621 tgcctgggtg ctgctgggtg tgttcctgtt cttcgtatcg accttcctgc tgggcgaagg
     1681 catcagccgc ctgggccggc tggcctatcc cgaccacgcg ctgctgacca tgaatatcgc
     1741 agctcgcaac gcgccgctga tgctggccgt gacgatggcg gcgctgcccg gccagccgct
     1801 gatctatgcc gcgctggtca ttggcatgct agtggagttt ccccatctaa ccgtattgca
     1861 gcggatattg cttggccagc ggcaatttcc aggatggtct ctgcgcccgt ccgccgagca
     1921 cccaggatcc acaagaacca agaagggaaa tctgcatcat gaccaccaag aacaagaccg
     1981 cgctcgttgt cagcgcccat tcggctgatt tgaccgagac ggcctgatcc cgtgcgtgac
     2041 caccgacgcg gcgaccggcg acgtgctgat gctgggctat atgaacgccg aggcattgtg
     2101 tcggaccatc gaagccggtg aggcgcacta ctggagccgg tcgctgtctg gctgcgagtg
     2161 cgggtcgccg gttccggcgc aagctgccat gtaggctatc gttcctgctt ctatcgcgat
     2221 gtacccaatc caactgtgct ttgagaggat gccatggcca caccgccact tcccgccagc
     2281 ctgcaggcct atcagttggg acgacgccac ggcgaccgga acctgaatcg caacctgggc
     2341 gtcaccctcc agccggcgga ttttgaagac cggatcgcca acgtgctgtg cccctggctg
     2401 caggcgcacg atgtgctgct ggatctgcat tcctttcaca caccaggcca gccgttcgcc
     2461 atgctggggc ctgaggataa tgcgggtgat ctggaaccct ttgcccatgc gagggccgag
     2521 gcgcagctcg cgacccatct tggcccgcgg cggctggtcg aaggctggat ggaagcctat
     2581 gcgcgcgggg tgcggcgccg gcgcgatcgc ggccagaccc gcacgcccgg gctgctcgac
     2641 gcccgctatg gggtcggtac gaccgaatac atgcgatcgg tgggcggccc tggaacagct
     2701 tcgacccggt gtcggccggc cagttgatcg gccaacgccg cgatgggcgt cccgtcctgg
     2761 ctcccgacga tggattcgtg atgtttccca atcccagtgc cctggcgggt aacgaatggt
     2821 tttactttgc ccagcgcagc gaccggcgcc tgccggcgtc catggtctta tagataagga
     2881 ggtatgttca cgatggccta cgattccgat aacgtctttg ccaagatcct gcgtggcgag
     2941 gccccgtcca ttcgggtcta tgaggatgcg gacacgctgg ccttcatgga catcatgccc
     3001 caggccgacg gccacgtgct ggtgatcccc aaggagcctg cggaaacctt gctggaactg
     3061 tcggaggaag cggcccgcgc ctgcatcgtc acgacccgcc ggatggcgat cgcggtccgg
     3121 acacaatgtt tcgggcgcac gcgagcgtgc gggccgaacc ggaacaactc gaaatgatcg
     3181 ctgcccgaat acgcgcatac ctgcaagacg gcgactgacc gggcctcgac ccaccacggg
     3241 ctgccctgcg ggcgcgactt atgccagagc gatcggcccg tgattgtcgc agtggtcgcc
     3301 gtgcggatgg tgcaggtggc cgtcgaccag gtaatccaca tggtcgccgt gcggcaccgc
     3361 ctggtggccg cactcggggc catgcacatg gccttggtcg tggcccgcgc aggcatgggc
     3421 aggggtgcag acgtccggat tcgtcgcgga cactgcgatg acatgttcgt cgatgtggtc
     3481 gccgtgcgga tggtggagat ggccgtcatg caggtagtcc acgtggcctt cgtgccgaat
     3541 ggcgatgtgg ccgcagttcg ggccgtgctg gtgagcgtgg ttcgtatgct gggggtggtt
     3601 gcaggtcgac atgtcgtgtt ccttgaagaa agcaatggcg gttattggcc cggtgccgcc
     3661 tcggccgcgt ggccgaggat gttgcgcagc agggcgtgga cgtgctcatc ggcgagcgcg
     3721 taaaaaatgt gcttggcctc acggcgtcgc gtcaccaggc gggccgcgtg cagggtttgc
     3781 aggcgggcgg aaatactgct gaccttggct tgctcgaatt caaccagctc ggatacgcac
     3841 atttcgcgct gggccagcca caacagcagc cgcaggcggg ccggatcgct cagcgcctgg
     3901 cacatggcgg cagcggtatc gagttgtcca ggcccgtagt cgatggccga gaccgtatcg
     3961 acatcgtgtt ggcaggcggt tgggtgtacg tggggcatct atcattccat taatacatgg
     4021 aatgataaaa tggcatccag cgttcgtcaa gaggccaacc aggccgattt ggtggatacc
     4081 cgtcaaaagc ctttgtcagg agcaccgagt ccgtcgctcg acagcccgcg gccatcttga
     4141 cggaaccggg tcgaccggcg taagtttacg tttacgtaaa ctgcaactta tcgcatgccg
     4201 cccacctgga ccatttccga gctctcgcgg gagttcgaga tcacgccgcg gacgatacgc
     4261 ttctacgaag accagggcat cgtcagcccc ggccgcgagg ggcgcaatcg cgtctacgac
     4321 gcgcgcgacc gcacgcgcct gaaactggcc ctgcgcggca agcgcctggg cctgcagctg
     4381 gccgagatcc gcgcgctgat cgatatgtac gacaccccgg gcgacaccga gctgcagctg
     4441 cgcgaatacc tgaacgtgct ggcccgccac cgctcggcgc tggagcagca gcgcctggac
     4501 atccaggaaa ccctgcagga aatcagcgaa caggagcggc tctgccagga gctgctgctg
     4561 gtcaagcaag cgtaaagcaa acgcaaagac acgcgaggag acaaaccatg aacattccgg
     4621 gcctgaactt cgacctgggc gaagacgtcg acatgctgcg cgaggccgtc gccgccttcg
     4681 ccgcggccga gattgcgccg cgcgcggccg agaccgaccg caccgaccag ttccccatgg
     4741 acctgtggcg caaattcggc gacctgggcg tgctgggcat gacggtgagc gaggaatacg
     4801 ggggcaccaa cctgggctac ctggcccaca tggtggcgat ggaagagatc tcgcgcgcca
     4861 gcgcgtccgt cggcctgtcc tacggcgcgc actccaacct gtgcgtcaac cagatccacc
     4921 gcaacggcaa cgaggcgcag aagcgcaagt acctgcccaa gctcgtcagc ggcgagcacg
     4981 tcggcgcgct ggccatgagc gagccgggcg cggggtcgga cgtggtcagc atgcgcctgc
     5041 gcgccgacaa gaagggcgac cgctacgtgc tcaacggcag caagatgtgg atcaccaacg
     5101 gccccgacgc cgacaccctg gtggtctacg ccaagaccga ccccgaggcg catcagcgcg
     5161 gcatcaccgc ctttctcatc gagaagggct ttgcgggctt ctccatcgcg cagaagctcg
     5221 acaagctggg catgcgcggc agccacaccg gcgagctggt gttccaggac tgcgaggtgc
     5281 ccgaggaaaa cgtgctcggc cagctcaacg gtggcgtccg cgtgctgatg agcgggctcg
     5341 actacgagcg cgccgtgctc tcgggcgggc cgctgggcat catgcaggcc gtcatggacg
     5401 tggccgtccc ctatatccac gaccgcaagc agttcggcca gtccatcggc gagttccagc
     5461 tcatgcaggg caagatcgcc gacatgtaca ccgtgctgca ggccagccgc gcctttgcct
     5521 acacggtggg caaaaacctc gaccgcctgg gcaaggaaca cgtgcgccag gtgcgtaaag
     5581 attgtgcatc actaatcctc tacactgccg agaaggccac ctggatggcc ggcgaaggcg
     5641 tgcagatcct gggcggcaac gggtacatca acgactaccc cacgggtcgc ctgtggcgcg
     5701 acgccaagct ctacgaaatc ggcgccggca ccagcgaaat ccgtcgcatg ttgataggcc
     5761 gggagctgtt caacgaaacg gtttgagttt gttttaggag tcacatcatg tccgatccga
     5821 tcgtcattgt ttccgtcgcc cgcacgccca tggg
//
LOCUS       DASWPO010000140         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2619993, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000140 DASWPO010000000
VERSION     DASWPO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619993"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VGC69_07810"
     CDS             <1..600
                     /locus_tag="VGC69_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0295223.1"
                     /translation="FKALGVGLAEDDLGSGHSSLARLQQLPFDTIKIDRDIVGSAAQD
                     PSQALRFIYQLTRLGHSLGKTVIVEGVEDLDMLEAIMVLGSDAVQGYVISRPMPAARL
                     TDWLRQAQPPMRLPMPGTVTSTLAKLAALLIWEERLHLMLDDINTIQMGLVPALPFNA
                     EPALQGALANAALEHGLRSSQYQGARALLVASLPGQASA"
     gene            complement(609..2042)
                     /gene="fliD"
                     /locus_tag="VGC69_07815"
     CDS             complement(609..2042)
                     /gene="fliD"
                     /locus_tag="VGC69_07815"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEY0295224.1"
                     /translation="MSSALITALGAGSNLDLQDILDKVKANEQQQLVALQSRQTKLAT
                     QISAYGAVQSALGTLQTAAQALIDPQLTNSAKVTVTGSDFTATAQPGATPMQSQVKVE
                     ALASTSQLLSGAISERAAAIGSGGTLAITLQDGSTASIDLQAGGSLDAIAKQINADGK
                     IGVQASIINDGTGSRYLMLSSRQTGTQAAVRSVTVSGNSALQSAIGYDADQPASSALT
                     LRHAATDAKVLINGVEITSGSNTLTKTIDQVTLTLTAVSKDDGGALTIEADTGASVQA
                     VQAFVDAYNTVIGKIASVSGYDAGSNTSAPLTGDGLLRGIQNTLSQALRVLVPDGGLK
                     TITDLGLDMTPLPAANGSGATVPAGGLLLNLDKLDSTHRNALSTALQQNAADAARVLK
                     TLGTQIAAATKGMLGKTGSIGSRTASLNNLSATLQDSYTKTAAHIDANIASMRTNFIK
                     LDVLISRMNSTSSYLTQQFAAWTNSKD"
     gene            2030..>2386
                     /locus_tag="VGC69_07820"
     CDS             2030..>2386
                     /locus_tag="VGC69_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295225.1"
                     /translation="MQKTCIAPVKLKFINLSENIQPRRSISEKFSNYSEIFVTLMVMT
                     IPPTDAALTADPACHRAQAEQARARGDELAALAHRLAARVLEANGADAGARLATVGTG
                     YFMKGEPAAARRWYELA"
BASE COUNT          352 a          778 c          830 g          426 t
ORIGIN      
        1 ttcaaggccc ttggcgtggg actggccgaa gacgatctgg gctcgggcca cagcagcctg
       61 gcgcgcctgc agcaactgcc gttcgacacc atcaagatcg accgcgacat cgtggggtcc
      121 gccgcccagg acccatccca ggccttgcgc ttcatctacc agctcacccg cttgggccac
      181 tcgctgggca agacggtcat tgtcgaaggc gtggaagacc tggacatgct cgaggccatc
      241 atggtgctgg gctcggatgc cgtgcagggg tatgtgatct cgcgccccat gccggcagcc
      301 cgcctgaccg actggctgcg gcaggcccag ccgccgatgc ggctgcccat gccgggcacg
      361 gtcaccagca cgctggccaa gctcgccgcc ctgctgatct gggaggagcg cctgcacctc
      421 atgctggacg acatcaatac catccagatg ggcctggtgc cggccctgcc cttcaacgcc
      481 gagcccgccc tgcaaggcgc cctggccaac gccgccctgg agcacgggtt gcgcagcagc
      541 cagtaccagg gcgcgcgggc gctgctggtg gcttcgctgc cgggccaggc ctcggcctga
      601 gccgccggct agtccttgct gttcgtccag gccgcgaact gctgcgtcag gtagctgctg
      661 gtggagttca tgcgcgaaat caggacgtcc agcttgatga agttggtgcg catgctggcg
      721 atgttggcgt cgatatgcgc cgcggtcttg gtataggagt cctgcagcgt ggccgacaag
      781 ttgttcaggc tggcggtgcg gctgccgatg gaacccgtct tgcccagcat gcccttggta
      841 gcggcggcga tctgggtgcc cagggttttc aggacgcggg ccgcgtcggc ggcgttctgc
      901 tgcaaggcgg tgctcagcgc attgcggtgg gtggaatcca gcttgtccag gttcagcagc
      961 aggccgccgg ccggcaccgt cgcgccgctg ccattggcgg cgggcagcgg cgtcatgtcc
     1021 aggcccaggt ccgtgatggt tttcaggccg ccgtccggca ccagcacccg cagcgcctgc
     1081 gacagcgtgt tctgtatgcc gcgcagcagc ccgtcgcccg tcagcggagc ggaggtgttc
     1141 gaaccggcgt cgtagcccga caccgaggcg atcttgccga tgaccgtgtt gtaggcgtcg
     1201 acgaaggcct gcacggcctg cacgctggcg cccgtgtcgg cctcgatggt cagcgcgccg
     1261 ccgtcgtcct tgctgacggc ggtgagcgtc agggtgacct ggtcgatggt cttggtcaag
     1321 gtattggacc cgctggtgat ctcgaccccg ttgatcagca ccttggcgtc ggtggccgcg
     1381 tgccgcagcg tcagggccga gctcgcgggc tggtcggcgt cgtagccgat ggccgactgc
     1441 agcgcgctgt tgcccgacac cgtgacggaa cgcaccgcgg cctgcgtgcc ggtctgccgc
     1501 gagctgagca tcaggtagcg gctgcccgtg ccgtcgttga tgatcgaggc ctgcacgccg
     1561 atcttgccgt cggcgttgat ctgcttggcg atggcgtcga gcgagccgcc ggcctgcagg
     1621 tcgatgctgg ccgtgctgcc gtcctgcagg gtgatggcca gggtgccgcc gctgccgatg
     1681 gcggccgcgc gctcggaaat ggcgccggag agcaactgcg acgtgctggc cagcgcctcg
     1741 accttgacct gggactgcat cggcgtcgcg ccaggctggg ccgtggccgt gaaatcgcta
     1801 cccgtcaccg tgaccttggc gctgttggtc agttgcgggt cgatcagcgc ctgggcggcc
     1861 gtctgcaggg ttccgagcgc ggattgcacg gcgccatagg cggaaatctg ggtggccagc
     1921 ttggtctggc ggctctgcaa ggcgaccagc tgctgctgct cattggcctt gaccttgtcg
     1981 aggatgtcct gcaagtcgag attggaaccc gcgccaaggg cggtgatgag tgcagaagac
     2041 atgcattgct ccggttaagc tgaaattcat taatctttca gaaaatattc aaccgaggcg
     2101 gagcatatca gaaaaattca gcaactattc agaaatattt gtcacgctca tggtcatgac
     2161 tatcccgccg accgatgccg ccctgaccgc ggaccccgcc tgccaccgcg cccaggcgga
     2221 gcaggcgcgc gcccgcggcg acgaactcgc cgcgctggcc catcggctcg ccgcccgggt
     2281 cctcgaagca aacggcgcag acgccggcgc gcgcctggcc accgtgggta ccggttattt
     2341 catgaagggc gagcctgcgg cggcgcggcg ctggtacgag cttgcg
//
LOCUS       DASWPO010000141         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1994978, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000141 DASWPO010000000
VERSION     DASWPO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994978"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VGC69_07825"
     CDS             complement(<1..750)
                     /locus_tag="VGC69_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295226.1"
                     /translation="MIDVQDVGIQFPGRDGPVQAVRHASFQVAAGEAYGLVGESGSGK
                     TTLLRAIGGLVAASAGRIVLGANTASTNAEKLSPRDVQMVFQDPYASLHPRFTVDRTL
                     REPLAINGLDKHDERIVDALRAVDLGPALRFRYPHQLSGGQRQRVAIARALIVEPRVL
                     LLDEPTSALDVSVQAEILNLLRGLHHDRGLTLVLVSHNLAVVGFLCTRIAVMRQGEIV
                     EQGNTAEVLHRPRDPYTAALLEAAMLRPKRAT"
     gene            complement(747..1619)
                     /locus_tag="VGC69_07830"
     CDS             complement(747..1619)
                     /locus_tag="VGC69_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295227.1"
                     /translation="MTTAPALCEIERLRIDFPNPDRQWSTAVRDVSLTLAPGERLGIV
                     GESGSGKSLTGRALLGLLPAAARMSARTLRYQGRDLLAMPPAERRRLCGREMGMILQD
                     PKYSLNPVMTVGQQMREAYALRDPGMKRREMDEAVLDALAAVHIRDPKRVSTSYPHEL
                     SGGMGQRVMIAMMASNGPRLLVADEPTSALDVLVAAQVLDVLDGMIARHGTGLIFISH
                     DLPLVGAFCDRVAVMYAGRVVETCAARDLHRARHPYTRALLAASPPLADPPERLPVMT
                     RDPAWLEPAGADAP"
     gene            complement(1622..2551)
                     /locus_tag="VGC69_07835"
     CDS             complement(1622..2551)
                     /locus_tag="VGC69_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295228.1"
                     /translation="MTGATDTVPRTGRRAWLLSAEPASRTQAALGQAYRRWRRLAANP
                     LSLCGLAVLMLLVLIALAGPLLPLPDPLRQVLADRLLPPGSPGHWLGTDQLGRDLLSR
                     LVHGARLTLAIAVLVVVVVVPIGLLVGTAAGFFGGWIDRALMRLTDIALAFPKIVLAL
                     ALAAALGPGVFNAVIAISITAWPAYARLARAETLRHAQADFIHAARMQGASRLRLLLR
                     YIMPLCASSVIVRATLDMAGIILSVAGLGFLGLGAQPPSPEWGYMVASGRGVLLDAWW
                     VATLPGLAILVASLALNLLGDGLRDVFDPRHGD"
     gene            complement(2548..3582)
                     /locus_tag="VGC69_07840"
     CDS             complement(2548..3582)
                     /locus_tag="VGC69_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295229.1"
                     /translation="MNPGKAAMRWGLRAAGWMLTLATTFAGLLAVTFFIGRKVPIDPV
                     LAVLGDRASASAYAAVRAQMGLDQPLPAQFAIYVREVLHGNLGTSLLTSNPVLDDIAR
                     VFPATLELATLATVIGILVGVPLGVAAARRHNRAVDHVARFVGLIGSSMPVFWLGLMG
                     LLLFYARLHWVAGPGRVDPAYDDMVDTRTGSLLIDALLAGEWDVFANALSHIVLPACI
                     LGYYSVAYLSRMTRAFMLEQLGQEYIATARVKGVPEWRVAWRHAFGNIAVPLLTVIAL
                     TYSNLLEGSVLTEIVFAWPGIGSYLTGALLNADMNAVLGCTLAIGALFILINLLTDVL
                     YRVFDPRVRT"
BASE COUNT          598 a         1268 c         1325 g          473 t
ORIGIN      
        1 cgttgcgcgt ttcggcctca gcatggctgc ctccagcagc gcggccgtgt acggatcgcg
       61 cggccggtgc agtacttccg cggtgttacc ctgctcgacg atctcgccct ggcgcatcac
      121 ggcgatgcgc gtgcacagga aacccaccac ggccaggttg tggctcacca gcaccagggt
      181 cagcccgcga tcatggtgca agccgcgcag cagattgagg atctccgcct gcaccgatac
      241 gtccagcgcc gaggtcggct cgtcgagcag cagcacgcgg ggctcgacga tgagggcgcg
      301 cgcgatggcc acccgctggc gctggccgcc ggagagctgg tgcggatagc gaaagcgaag
      361 ggccggaccc aggtccaccg cgcgcagggc gtcgacgatg cgttcgtcgt gcttgtccag
      421 gccgttgatg gccaggggtt cgcgcaaggt gcggtccacc gtgaagcgcg gatgcaagga
      481 cgcataaggg tcctggaaca ccatctgcac gtcgcgcggc gacaattttt ccgcattcgt
      541 gcttgccgtg ttcgcgccga gcacgatgcg tcccgcgctg gcggcgacca gtccgccgat
      601 ggcgcgcagc agcgtcgtct tgcccgaacc cgattcgccc accaggccat aggcctcgcc
      661 cgcggcgacc tggaagctgg catggcgcac cgcctgcacc gggccgtcgc ggccggggaa
      721 ttggatgccc acgtcctgca cgtcgatcat ggcgcgtctg ctccggcggg ttccagccag
      781 gccgggtcgc gcgtcatcac gggcaatcgt tcgggcggat cggccagcgg cgggctggcg
      841 gcgagcaggg cgcgcgtgta ggggtgccgc gcccggtgca ggtcccgcgc ggcgcaggtc
      901 tcgaccacgc ggccggcata catcaccgcg acccggtcgc agaaggcgcc caccaggggc
      961 aggtcgtggc tgatgaagat caggcccgtg ccgtgccggg cgatcatgcc gtccagcacg
     1021 tccagcactt gcgcggccac cagcacgtcg agcgcgctgg tgggttcgtc ggccacgagc
     1081 aggcgcgggc cgttggacgc catcatggcg atcatcaccc gctgccccat gccgcccgac
     1141 agctcatgag gataggaggt actcacgcgc ttcggatcgc ggatgtgcac cgcggccagc
     1201 gcgtcgagca cggcttcgtc catctcgcgc cgtttcatgc cgggatcgcg cagcgcgtag
     1261 gcctcgcgca tttgctggcc gacggtcatg acggggttca gcgaatactt cgggtcctgc
     1321 aggatcatgc ccatttcccg cccgcacagg cggcggcgtt cggccggcgg catggcgagc
     1381 aggtcgcgcc cttggtagcg cagggtccgc gccgacatcc gcgccgcggc gggcagcagg
     1441 cccagcagcg cccggccggt cagggacttg cccgaacccg actcgccgac gatgcccagg
     1501 cgctcgcccg gcgccagcgt gagcgacacg tcgcgcacgg ccgtcgacca ctgccggtcg
     1561 gggttgggaa aatcgatacg cagccgctcg atctcgcaaa gcgcgggggc cgtcgtcatg
     1621 ttcagtctcc gtggcggggg tcgaaaacat cgcgcaggcc gtcgcccagc aggttcaggg
     1681 ccaggctcgc caccaggatg gccaggccgg gcagcgtggc cacccaccag gcatcgagca
     1741 gcacaccgcg gcccgaggcc accatatagc cccattccgg gctgggcggc tgcgcgccca
     1801 ggcccaggaa gcccaggccc gccacgctca ggatgatgcc ggccatgtcc agcgtggcgc
     1861 gcacgatcac cgacgaggcg cacagcggca tgatgtagcg cagcagcagg cgcagccgcg
     1921 aagcgccctg catgcgggcg gcatggatga agtcggcctg cgcgtggcgc agcgtctcgg
     1981 cgcgggccag ccgcgcatag gccggccagg ccgtgatgga aatggcgatc accgcgttga
     2041 acacgccggg ccccagcgcg gcggccagcg ccagggccag cacgatcttg ggaaaggcca
     2101 gcgcgatatc ggtcaagcgc atcagcgccc ggtcgatcca gccgccgaag aatcccgcgg
     2161 ccgtgccgac cagcaggccg atgggcacca ccaccacgac caccagcacc gcaatggcca
     2221 gggtcaggcg cgcgccatgc accaggcgcg agaggaggtc gcggccgagc tggtcggtgc
     2281 cgagccagtg gcccgggctg ccgggcggca gcaggcggtc ggccaggacc tgccgcagcg
     2341 ggtcgggcag cggcagcagg gggcctgcca gggcgatgag gaccagcagc atcagcaccg
     2401 ccaggccgca cagcgacaag gggttggcgg ccagccggcg ccagcgccgg taggcctggc
     2461 ccagcgcggc ctgggtgcgc gaggcgggct cggcgctgag cagccaggcg cggcggccgg
     2521 tgcgcggcac ggtgtctgtc gcgcccgtca tgtgcgcacc ctcgggtcga agacgcgata
     2581 cagcacgtcg gtgagcaggt tgatgaggat gaacagggcg ccgatcgcca gggtgcagcc
     2641 cagcacggcg ttcatgtcgg cattgagcag cgcgccggtg agataggagc ctatgcccgg
     2701 ccaggcgaag acgatctcgg tgagcaccga tccttccagc aggttgctgt aggtcagcgc
     2761 gatgacggtc aacagcggca cggcgatatt gccgaaggca tggcgccacg ccacgcgcca
     2821 ttccggcacg cccttgaccc gcgcggtggc gatgtactcc tggccgagct gctcgagcat
     2881 gaaggcgcgc gtcatgcggc tgagataggc caccgagtaa tagcccagga tgcaggccgg
     2941 cagcacgatg tgcgagagcg cgttggcgaa gacgtcccac tcgcccgcca gcagcgcgtc
     3001 gatcagcagg ctgccggtgc gcgtgtcgac catgtcgtcg taggccgggt ccacccggcc
     3061 ggggccggcc acccagtgca gccgggcgta gaacagcaag aggcccatca ggcccagcca
     3121 gaagaccggc atggagctgc cgatcaggcc cacgaagcgg gccacgtggt cgacggcgcg
     3181 gttatggcgc ctggcggccg ccacgcccag cggcacgccg accaggatgc cgatgaccgt
     3241 ggccagcgtg gccagttcga gcgtggcggg gaacacgcgc gcgatatcgt cgagcaccgg
     3301 attggacgtg agcagcgaag tgcccaggtt gccgtgcagc acctcgcgca catagatggc
     3361 gaactgcgcg ggcagcggct ggtccagccc catctgcgcg cggaccgccg cataggcgct
     3421 ggccgaggcc cggtcgccca gcacggccag caccgggtcg atgggcacct tgcggccgat
     3481 gaagaaggtc acggccagca ggccggcgaa agtggtcgcc agcgtcagca tccagccggc
     3541 ggcgcgcagg ccccagcgca tcgcagcctt gcccggattc attgcttctt caagtggcgg
     3601 tacgacacca ggtcgttgat cgggccgacc tcgatgccgc ccacgcccgg cagcatggtc
     3661 acct
//
LOCUS       DASWPO010000142        14984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1281888, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000142 DASWPO010000000
VERSION     DASWPO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14984
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281888"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1414)
                     /locus_tag="VGC69_07845"
     CDS             complement(122..1414)
                     /locus_tag="VGC69_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEY0295230.1"
                     /translation="MVQLILLLLGVDYLRRRSRALAIAGMVFLLAGVVLFADALDGAV
                     YFPLAVFAVLFVVEGLATLCVATTGVGGQRVLRYVKGGFVLLAGVLILAGDHHGHFVL
                     SMIFGLLFLVDGLMQCLASYMVRYGRWRYVFAAGVAEILLAVFFFQPYPTHYVGTLPY
                     CLGLFLFFAGLKLLSLAARVRRLSANPAFVPDPGLGFAPAAGAPASRRTVWDGPPAPS
                     EKALTVHVWTPAGSAKAETRNYPVVNRYIAAVDAHGVVSTGHAALESPEGIYVSLYPG
                     VEIDHSPSEFGTLLRATAENDIPGVFQPDYATESKAWCPSTVQVRIRNYDSRKLGEFW
                     EAYRRDTTYNLTHRNCSSSVSAALDAALDGTVGRLAGRGGGVFLRLLLTPELWVASQI
                     RKRATTMSTMPSPPLPVKVRHHSVASFMLVSLFSSTIL"
     gene            1531..3006
                     /gene="pap"
                     /locus_tag="VGC69_07850"
     CDS             1531..3006
                     /gene="pap"
                     /locus_tag="VGC69_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HEY0295231.1"
                     /translation="MFEEAESDPRLPKDLFKHQEPRLRTALLKAQYQLLDRADRSLLI
                     VIAGIDGAGKGDTVNLLNEWMDPRHIHTLAFDTPTPEEAERPPFWRYWTALPAKGRIG
                     IVFGSWYVPLLREAARKHPDAARIDQEAEHIRRFEAELSANGVQIIKLWYHLSAQAQA
                     ERAKTLLSSPETAWKVMPVDLKVPKKFDRLRNAGQAAITHTDTAHAPWSVVPSADAQV
                     RHLRTAEAVLAALRRRHAPRPVRIHVPGEGRKHVRIIDRLGKLDYEARFEKDDYETEL
                     GLLQGRLARATRSPKFHGKHSLVLAFEGQDAAGKGGAIRRVTHALDARQYEITPIAAP
                     GSYEQARPYLWRFWRHVPALGRVAIFDRSWYGRVLVERVENITTPADWRRAYGEINNF
                     EEQLTDSGAIVLKFWLAVTAGVQLERFHEREHSPFKNFKITPEDWRNRKRWKDYAAAT
                     NEMLARTSTPAAPWHLISANDKRYARLQVLRHIAEAMEARL"
     gene            3026..3472
                     /locus_tag="VGC69_07855"
     CDS             3026..3472
                     /locus_tag="VGC69_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0295232.1"
                     /translation="MTVQLRPVTADDFNAWLPLWKGYQAFYHVDIADEVTRSTWQRLL
                     DPAEPMNAALAAVDGRAVGLVHWIYHRSTWTAGNYCYLQDLFVAPDVRGTGAGRALIE
                     HVYADAARQKAARVYWLTHETNHSAMQLYDRIADKPGFVQYRKLIG"
     gene            3506..4267
                     /locus_tag="VGC69_07860"
     CDS             3506..4267
                     /locus_tag="VGC69_07860"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEY0295233.1"
                     /translation="MATLGILAALPDEIRALLDEMGPGATTRRIGMRDYHQGVLHGQP
                     CVIALTRVGKVAAAATAATLLHEFKVDRVVFTGLAGGVATKVQVGDVVVGSALVQHDL
                     DARPLFPRHEVPWLHVDRFATSDALSALLLRCAAEFLAEVPTAPAVHLGLIATGDQFI
                     NNAGTVAQLRADIPEALCVEMEGAAVAQVCYEFNVPCAVLRTISDRADADSHLDFPIF
                     LREVASVYSAGILRRFLAALPALDQAMEQPDAPTA"
     gene            4282..5031
                     /locus_tag="VGC69_07865"
     CDS             4282..5031
                     /locus_tag="VGC69_07865"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0295234.1"
                     /translation="MLFSDTAPHSNRLSASTLAAEAARLPAPWRTALQSPRAAQALQS
                     VTAFVEERLAQGATVYPARPFRALQELLPAQVRVVILGQDPYHGPGQAQGLSFSVPDD
                     CPTPRSLRNIQKEIARDVPGARIAGNDLAPWAAQGVLLLNTALTVEDGQAGAHARRGW
                     EDVTDALIGAVADEPHPKVFMLWGGHAQAKEGLLPAGGPHLVLKANHPSPLSASRPPI
                     PFLGCGHFSRANAWLQAQNAHAIDWSTGISG"
     gene            5402..7273
                     /locus_tag="VGC69_07870"
     CDS             5402..7273
                     /locus_tag="VGC69_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0295235.1"
                     /translation="MSFAELGLAPALVSALTEAGLETPTTVQAAAIPQALQGHDLMVS
                     SQTGSGKTAAFMLPALNRIANQSGQGGTGVQVLVLAPTRELAQQVADATQVYGSKLSD
                     LRVATVVGGVPYGAQLKALSRRIDVLVATPGRLIDHIKAGRVKLNTVHTLVLDEADRM
                     LDMGFIEDIETIIERTPGERQTLLFSATLDGTVAKLAASMMREPQRIEMSNQRDKHTN
                     ITQTLLYADDQAHKMRLLDHVLRDVKLEQAIVFTATKRGADELADRLSGQGFAAASLH
                     GDMNQRQRTRTLTQMQRGQVRVLVATDVAARGIDVQGISHAINYDLPLQAEDYVHRIG
                     RTGRAGRDGLAFTLAVHGERHKVRRIESYIGQTLNAEIIAGLEPQRSARPDAPRHGKP
                     GGGKFQGQRPGGYDRAPRKFDDRGDRPQRTFSDRPAFGDRAPHEGGKFQGQREGFNGQ
                     RREFGDRPQRSFGDRPAAARPAGDRPQRDFGDRPARPFSDRPAFGDRAPREGGKFQGQ
                     REGFNGQRREFGDRPQRSFGDRPVAARPAGDRPQRDFGDRPVRSFGDRPAFGDRAPRE
                     GANGQRREFGDRPQRSFGDRPAVARPVGDRPAFGAKPRGDKPFEKRAGGPKRFERRG"
     gene            7354..8541
                     /locus_tag="VGC69_07875"
     CDS             7354..8541
                     /locus_tag="VGC69_07875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0295236.1"
                     /translation="MQRTPPSSLPALDAASAAHSADVAEHLRGVIRVAGDWLPFHLWM
                     AHALYAPGLGYYTAGAVKLAAGGDATRDLPQGDFVTAPELTPLFACTLAQQAHQVLQA
                     TGTRTVLEFGAGSGALAEGVLRELDRLGHPVQYQIMEVSADLRQRQQARLAAYGERVQ
                     WLDALPAAFEGCVLANEVLDAMPVSLFAWNDAGRVMQRGVALDATGGFIWEDRTAPAV
                     LAKAVAARMPPLPGYVSEINLQAEAWMREMGGWLARGAALIIDYGFPQREYYHPQRLG
                     GTLMCHLRHHAHADPFVAPGLQDITAHVDFTALADAALDGGLEVLGYASQAHFLINAG
                     LLQILGRQDTGDVQAYAQLAAPVQKLLSEAEMGELFKVLAVGRAVDASLIGFARGDRR
                     NSL"
     gene            complement(8538..9665)
                     /locus_tag="VGC69_07880"
     CDS             complement(8538..9665)
                     /locus_tag="VGC69_07880"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165686.1"
                     /note="catalyzes the addition and repair of the
                     3'-terminal CCA sequence in tRNA; these proteins belong to
                     the CCA-adding enzyme subfamily 2 which does not have
                     phosphohydrolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY0295237.1"
                     /translation="MNPIDPAPESAADPATAGLQAYVVGGAVRDALLGLPAGDRDWVV
                     VGATPEDMARRGFTPVGGDFPVFLHPVTKEEYALARTERKSGRGYKGFTFYTGADVTL
                     EQDLRRRDFTVNAMARSREGVLADPLDGVADLRARVFRHVGEAFAEDPVRILRLARFM
                     ARFTDFGVAGETMALCRQMVGAGEADALVPERVWKEVSRGLMSALPSRMLDVLAQSGA
                     LARVMPELQAAAQVGAEVDRAAAAGLPLPSRYALLSRLSERREALGKRLRVPADCADQ
                     ARLLPGLVDGLPGATDAQGRLALIEQCDALRKPERFMALLRAAAVVGAPVDEAAWQRR
                     LDAVRGIDAGAIAQACAGDPARIKPALRQARLDALRQGPEA"
     gene            complement(9712..11868)
                     /locus_tag="VGC69_07885"
     CDS             complement(9712..11868)
                     /locus_tag="VGC69_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0295238.1"
                     /translation="MTQDPETARYQKSASPVRPSAAWVVSRIRLWLPMLAMVIAACSS
                     VDAQQSTGAPAAAQAPALASLATGPLPVFHAAIQPRPVQAGLPPSPDTPARQALIDAH
                     DAQGRPQWGALAQAAAAQARDDPLGIYAEYWLLRYKLAFPLPGSHPFDDAQQFLDAHP
                     QSYLADKLRADLILMAAARGDFQAVQDLGEPINRGPAVACARLDAQHMTGRRATAEQA
                     LAVFQPVRSCWKLYDQLVADGVLGWDQIMPLLRDAIEDNRIPAATQLAGYIFENNDRK
                     KLAVLLKDPLKWLARQSKSVVGRNEKALLSIALARLARQDLNTADAYFRNEWERYMAK
                     GEAAWVRSQYALAAAYAQDDRAEQWYREAGDVRLSKTGQAWRVRSALRAARIDWKWVL
                     ACIAQMPPEQQAEPGWVYWKARGLAASGQADAASQAYAGIADQFNFYGQLASEELGRP
                     IVLPPEAAPVTSAEMAEARRNPGLVRAVQLFRLGWRAEAVPEWNFALRGMTDRQLLAA
                     AELARAEDIYDRVVNTSELTAHEFDFTQRYIAPFDGKVAAKAQAISLDPAWVYGLIRQ
                     ESRFVTQAHSYVGAAGLMQIMPATAKWVAKKIGMTDFDPTRVHDFDVNTELGTQYLNI
                     VLQAVDGSQMLASAGYNAGPGRPRNWRAALSHGVEGAIFAETIPFNETRDYVQKVMAN
                     ATYYAVIFTGRPQSLKARLGLVDPGQSQVLANSDTQ"
     gene            11953..12627
                     /locus_tag="VGC69_07890"
     CDS             11953..12627
                     /locus_tag="VGC69_07890"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0295239.1"
                     /translation="MPNQTSAQNTAAALRARLKQARADLPDDERDRGALLMRGRLFSW
                     LNAARDALAPSGRTISSVAAFWPLAGEPDLRPLLAQWSDHGVQVCLPCVTGPGQPLEF
                     RNWTPAAPMAAGAYGIAEPAAGPACVPDVILVPTLGYTDRADRLGYGGGYYDRTLAAL
                     DEAGHGCIAIGIAWTEGRLPEDYEPAAHDRRLDAILTPDGWLPNAPLGAAPDSARSRI
                     MRFILG"
     gene            12847..14280
                     /locus_tag="VGC69_07895"
     CDS             12847..14280
                     /locus_tag="VGC69_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY0295240.1"
                     /translation="MHDAAPRPAAAYDEMYDAQGQVRPQYRAYADWLDSQSSDAMAAR
                     QIEADLSFRRVGITFSVAGDAAGTERLIPFDLIPRIIPANDWQRLEAGLKQRVRALNM
                     FIHDIYHGHDIVHAGVVPAEHVFLNAQYRPEMQDIDVAEDIYSHIAGIDLVRAGQGEF
                     YVLEDNLRVPSGVSYMLEDRKMSMRLMPDVFSKVKVAPVAHYPDLLLDNLREVAPRSI
                     DDPTVVVLTPGMYNSAYFEHAFLAQQMGVELVEGQDLFVEQNTVYMRTTHGPQRVDVI
                     YRRLDDDFLDPLSFRADSVLGVPGLLSVYRAGRVTLANAIGTGIADDKATYLYVPDMI
                     KFYLSEEPILSNVPTWQCAKPAELSHVLANMHELVVKEVHGAGGYGMLVGPTSSKAQV
                     EDFKTRVKANPANYIAQPTLALSTVPTYVESGVAPRHVDLRPYVLCGKDIRTVPGGLC
                     RVALVEGSLVVNSSQGGGTKDTWVLED"
     gene            14282..>14984
                     /locus_tag="VGC69_07900"
     CDS             14282..>14984
                     /locus_tag="VGC69_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY0295241.1"
                     /translation="MLSRTADNLFWMCRHIERAENTARMLDVNLQMSLLPQDLQTLEN
                     SWRAVLRISELQGGYDARYPGITPQDVLHYMVREADNPSSIYACLRGARENARAVRGS
                     LTTEVWETYNSTWLGLLQHLDNGLPERNPGEFFEWVKFRAHLARGVTVATMQTDEALY
                     FLRLGMHLERADNTARMLDVKFHERDDDMPAGRAAGADADNGMYSEFYRWSALLSSMS
                     GMEIYRKIYRDVIAPD"
BASE COUNT         2479 a         5564 c         4762 g         2179 t
ORIGIN      
        1 tcgcaccatt ccacatctta gcatccgaag gaaagctttg gacgagcgag aaccacgaat
       61 ggcatatgga acaaataaaa aaggtttgcg aggccgatcc tgctatattg ttagctacac
      121 cctataagat agtggaactg aataatgaaa ccagcataaa cgatgctaca gagtggtggc
      181 gaactttaac tggtaaaggc ggggaaggca tggtagacat cgtcgtggcg cgcttgcgga
      241 tctgcgaggc gacccacagc tcgggcgtca acagcaggcg caggaatacc ccgccgcccc
      301 ggccggccag ccgccccacg gtgccgtcca aggccgcgtc cagcgcggcc gacacgctgc
      361 tggagcaatt gcgatgggtc aggttgtagg tggtgtcccg gcggtatgcc tcccagaatt
      421 cgcccagctt gcgcgaatcg taattgcgga tccgcacctg cacggtcgaa ggacaccagg
      481 ccttggattc ggtcgcatag tcgggctgga acacgccggg gatgtcgttc tcggccgtgg
      541 cgcgcagcag cgtgccgaac tcggagggag aatggtcgat ctccacgccc gggtacaggc
      601 tgacatagat cccttccggc gactcgagcg ccgcatggcc ggtggacacc acgccatggg
      661 cgtcgaccgc cgcgatgtag cggttgacca ccggatagtt cctggtctcg gccttggccg
      721 aaccggcggg cgtccagacg tgcacggtca gcgccttttc ggagggcgcg ggcgggccgt
      781 cccagacggt ccgccgcgat gcgggcgcgc ccgccgccgg cgcgaagcca aggcccggat
      841 ccggcacgaa cgccgggttg gccgacaggc gccgcacccg cgcggccagc gacagcagct
      901 tcaaccccgc gaagaacagg aacaggccca ggcaataagg cagcgtgccg acatagtggg
      961 tggggtaggg ctggaagaaa aacacggcga gcaggatctc cgccacgccg gcggcgaaga
     1021 catagcgcca gcgcccgtag cgcaccatat agctggcgag gcactgcatc aggccgtcga
     1081 ccaggaacag caggccgaag atcatcgaca ggacgaaatg gccatgatgg tccccggcca
     1141 ggatcaacac cccggccagc agcacgaagc cgcccttgac gtagcgcagc acgcgctgcc
     1201 cgccgacacc cgtggtggcg acgcacagcg tggccaggcc ctcgacgacg aacaggacgg
     1261 cgaacacggc cagcgggaaa tacacggccc cgtccagcgc gtcggcgaaa agcacgacgc
     1321 cggccagcag gaataccatg ccggcgatgg ccagcgcgcg gctgcgccgg cgcaggtagt
     1381 cgacgcccag cagcagcaag atcagttgca ccatgtatcc gtctctcccg tcccgttcag
     1441 cttatagctt aagcccaacc gacaaacgac ttgcacgccg ccagcgaatc ccgcaacatc
     1501 gccgcatcct ccatccagcg gaccccggcc atgttcgaag aagcagaatc cgacccccga
     1561 ctccccaagg acctcttcaa gcaccaggaa ccccgcctgc gcaccgcgct gctcaaggcg
     1621 caataccaac tgctggaccg cgccgaccgt tcgctcctga tcgtcatcgc cggcatcgac
     1681 ggcgccggca agggcgacac cgtcaacctg ctcaacgaat ggatggaccc gcgccacatc
     1741 cacaccctgg ccttcgacac ccccaccccc gaagaagccg aacgcccgcc gttctggcgc
     1801 tactggaccg ccctgccggc caagggccgc atcggcatcg tcttcggctc ctggtacgtg
     1861 cccctgctgc gcgaagccgc ccgcaagcac cccgacgccg cccgcatcga ccaggaggcc
     1921 gaacacatcc gccgcttcga ggccgagctt tcggccaacg gcgtgcagat catcaagctc
     1981 tggtaccacc tctccgccca ggcccaggcc gaacgcgcca agacgctgct gtccagcccc
     2041 gagaccgcct ggaaagtcat gccggtggac ctcaaagtcc ccaagaaatt cgaccgcctg
     2101 cgcaacgccg gccaggccgc gatcacgcac accgacaccg cccatgcgcc ctggagcgtg
     2161 gtgcccagcg ccgatgcgca ggtgcgccac ctgcgcacgg ccgaagccgt gctggccgcg
     2221 ctgcgccgcc ggcatgcgcc gcgcccggtg cgcatccatg tgccgggcga aggccgcaag
     2281 cacgtgcgca tcatcgaccg gctcggcaag ctcgactacg aagcgcgctt cgagaaagac
     2341 gactacgaga ccgagctcgg cctgctgcaa ggccggctgg cgcgggccac gcgctcgccc
     2401 aagttccacg ggaagcactc cctggtcctg gccttcgaag gccaggacgc ggcgggcaag
     2461 ggcggcgcca tccggcgcgt cacccatgcg ctggacgccc ggcagtacga gatcacgccc
     2521 atcgccgcgc cgggctccta cgagcaggcg cgcccctatc tgtggcgctt ctggcgccat
     2581 gtgccggcgc tgggccgcgt cgccatcttc gatcgctcct ggtacggccg cgtactggtc
     2641 gagcgcgtgg aaaacatcac cacccccgcc gactggcgcc gcgcctacgg cgagatcaac
     2701 aacttcgagg aacagctgac cgacagcggc gccatcgtgc tgaagttctg gctggccgtc
     2761 accgccggcg tgcagctcga gcgattccac gaacgcgagc attcgccctt caagaacttc
     2821 aagatcacgc ccgaagactg gcgcaaccgc aagcgctgga aagactacgc ggccgccacc
     2881 aatgaaatgc tggcccgcac cagcacgccc gccgcgccct ggcatttgat atcggccaac
     2941 gacaagcgct atgctcggct gcaggtgctg cgccatatcg ccgaggcgat ggaagcccgt
     3001 ctctaacccc cttgccccgc catccatgac cgtacaacta cgccctgtca ccgccgacga
     3061 tttcaatgcc tggctgccgc tctggaaggg ctatcaggcg ttctaccacg tcgatatcgc
     3121 cgacgaggtc acccgctcca cctggcagcg cctgctcgac ccggccgaac ccatgaacgc
     3181 cgccctggcc gccgtcgacg gccgcgccgt gggcctggtg cactggatct accatcgcag
     3241 cacctggacc gccggcaatt actgctacct gcaagacctg ttcgtcgcgc ccgatgtgcg
     3301 cggcacgggc gcgggccgcg cgctcatcga acatgtctac gccgacgccg cccggcagaa
     3361 agcggcgcgc gtgtattggc tcacccacga aactaaccac agcgccatgc agctgtacga
     3421 ccgcatcgcc gacaagcccg gcttcgtcca ataccgcaag ctgatcggct gaggcataca
     3481 cggtttacct gcccggaagt ccatcatcgc aaccctaggc atactcgccg ccctgcccga
     3541 cgaaatccgc gcgctgctgg atgagatggg ccccggcgcc accacgcggc gcatcggcat
     3601 gcgcgactac caccagggcg tgctgcatgg gcagccctgc gtgatcgcgc tcacgcgcgt
     3661 gggcaaggtg gcggccgcgg ccaccgccgc cacgctgctg cacgagttca aggtcgatcg
     3721 cgtggtgttc accggcctgg cgggcggcgt ggccaccaag gtacaagtcg gcgacgtggt
     3781 cgtcggcagc gccctggtgc agcacgacct cgacgcccgc ccgctgttcc cgcggcacga
     3841 agtgccctgg ctgcacgtcg accgcttcgc cacctcggat gcgctctcgg ccctgctgct
     3901 gcgctgcgcc gccgagttcc tcgccgaggt gcccacggcg cccgccgtgc acctgggcct
     3961 gatcgccacc ggcgatcagt tcatcaacaa cgccggcacc gtggcgcaac tgcgcgccga
     4021 catacccgag gccctgtgcg tcgaaatgga aggcgcggcc gtggcccagg tctgctacga
     4081 attcaacgtg ccctgcgccg tgctgcgcac catctccgac cgggccgacg cagactcgca
     4141 cctcgacttt ccgatttttt tgcgcgaagt ggccagcgtg tactcggccg gcatcctgcg
     4201 ccgcttcctg gccgccctgc cggcgctcga ccaggccatg gaacaacccg acgcccccac
     4261 cgcctgacac gccgactccc gatgcttttc tccgacaccg ccccccacag caaccgcctg
     4321 agcgccagca ccctggccgc cgaggccgcc cgcctgcccg cgccctggcg caccgccctg
     4381 caatcccccc gcgccgcgca ggcgctgcag tcggtcaccg ccttcgtcga agaacggctg
     4441 gcccagggcg cgacggtcta cccggcccgt cccttccgcg cgctgcagga actcctgccg
     4501 gcccaggtgc gcgtggtgat cctgggacag gacccctatc acggccccgg ccaggcccag
     4561 ggcctgtcct tttccgtgcc cgacgactgc cccacgccgc gcagcctgcg caacatccag
     4621 aaggaaatcg cccgcgacgt gccgggcgcc cgcatcgccg gcaacgacct cgcgccctgg
     4681 gcggcccagg gcgtgctgct gctcaacacc gcgctcaccg tcgaggacgg ccaggccggc
     4741 gcccatgcca ggcgcggctg ggaagacgtc accgatgccc tgatcggcgc cgtggccgac
     4801 gagccgcacc ccaaggtctt catgttgtgg ggcggccacg cccaggccaa ggaagggctg
     4861 ctgccggccg gcggcccgca tctcgtgctc aaggccaacc acccttcgcc cctgtccgcc
     4921 agccggccgc cgattccctt cctgggctgc gggcatttca gccgggccaa tgcctggctg
     4981 caggcccaga atgcacacgc catcgactgg tctacaggaa tttcgggtta accctagaca
     5041 caatgcagcg aaccgggtta tgattgcggc actgcactat ttgattcggc atactttctt
     5101 tcaggccttt gcagttcgcg gctcgtcgca ccctcaaggc cccctgcagt ccgtcccgtt
     5161 acttggccct ccgcctggca ccgcaacaca agccagcgca cctcccggtc aaaccggtcc
     5221 gatgccgaat ctcatcgatt cctgacctct ttcctggcgc tttttgcgtt cgttgtctga
     5281 acgttgccgc gcgtacggtt gatttggtcg gcacgatgct tatcaaccgt agctccggac
     5341 cggctcacca acctcctgcc cgtcccgtgc gcaacgccgc caaaagcggc aagggaaagt
     5401 catgtctttc gcagaactgg gcctcgcgcc cgcacttgtc tcggccctca ccgaagccgg
     5461 tctcgagacc cccaccaccg tccaggccgc cgccatcccg caagccctgc aagggcacga
     5521 cctgatggtc tcgtcgcaga ccggcagcgg caagaccgcc gccttcatgc tgccggcgct
     5581 caaccgcatc gccaatcaat ccggccaggg cggcacgggc gtgcaggtgc tggtgctggc
     5641 ccccacccgc gaactggccc agcaagtggc cgacgccacc caggtctacg gcagcaagct
     5701 gtccgatctg cgagtggcca ccgtggtcgg cggcgtgccc tacggcgccc agctcaaggc
     5761 cctgtcgcgc cgcatcgacg tgctggtggc cacgcccggc cgcctcatcg accacatcaa
     5821 ggccggccgc gtcaaactca acaccgtgca caccctggtg ctcgacgaag ccgaccgcat
     5881 gctcgacatg ggtttcatcg aagacatcga aaccatcatc gagcgcaccc ccggtgaacg
     5941 ccagaccctg ctgttctcgg cgacgctgga cggcaccgtg gccaagctgg ccgcgagcat
     6001 gatgcgcgag ccgcagcgca tcgagatgtc caaccagcgc gacaagcaca ccaacatcac
     6061 ccagacgctg ctctacgcgg acgaccaggc ccacaagatg cgcctgctcg accacgtgct
     6121 gcgcgacgtc aagctggaac aggccatcgt cttcaccgcc accaagcgcg gcgccgacga
     6181 gctggccgac cgcctgagcg gacaaggctt tgccgccgcc tctctgcacg gcgacatgaa
     6241 ccagcgccag cgcacccgca ccctcaccca gatgcagcgc ggccaggtgc gcgtgctggt
     6301 ggccaccgat gtcgccgccc gcggcatcga cgtgcaaggc atcagccacg ccatcaacta
     6361 cgacctgccc ctgcaggccg aagactacgt gcaccgcatc ggccgcaccg gccgcgccgg
     6421 ccgcgacggc ctggcgttca ccctggccgt ccacggcgaa cgccacaagg tgcgccgcat
     6481 cgaaagctat atcggccaga ccctcaacgc cgagatcatc gccggtctcg agccccagcg
     6541 ctcggcgcgt cccgacgccc cccgccacgg caagcccggc ggcggcaagt tccagggcca
     6601 gcgccccggc ggctatgacc gcgccccgcg caagttcgac gaccgcggcg atcgtccgca
     6661 gcgcactttc agcgaccgtc ccgcctttgg cgaccgcgcc ccccacgaag gcggcaagtt
     6721 ccagggccag cgcgaaggct tcaacggcca gcgccgcgaa ttcggcgacc gcccccagcg
     6781 cagtttcggt gatcgtcccg ccgccgcgcg cccggccggc gaccgcccgc aacgcgattt
     6841 cggcgatcgt cccgcccgtc cgttcagcga ccgccccgcc ttcggcgacc gcgccccccg
     6901 cgaaggcggc aagttccagg gccagcgcga aggcttcaac ggccagcgcc gcgaattcgg
     6961 cgaccgcccc cagcgcagct tcggtgatcg tcccgtcgcc gcgcgcccgg ccggcgaccg
     7021 cccgcaacgc gatttcggcg atcgtcccgt ccgttcgttc ggcgaccgtc ccgcctttgg
     7081 cgaccgtgct ccccgcgaag gcgccaacgg ccagcgccgt gaattcggcg accgtccgca
     7141 gcgcagcttc ggcgatcgtc cggccgtcgc ccgcccggtt ggcgaccggc cggccttcgg
     7201 cgccaagccg cgcggcgaca agcctttcga aaagcgcgcc ggcggcccca agcgcttcga
     7261 acgccgcggc tgatcctgcc ttgtaggtag caagacggcc ctgccccttc gggagcaggg
     7321 ccgtcttgct ttgggcgtgg gaaaatacgc ctcatgcaac gcacgccccc ctcctccctc
     7381 cccgcgctgg atgccgccag cgccgcgcac agcgccgacg tcgccgagca cctgcgcggc
     7441 gtgatccgcg tcgccggcga ctggctgccc tttcacctgt ggatggccca cgcgctgtac
     7501 gcgccgggcc tgggctatta cacggcaggc gccgtcaagc tggccgcggg cggcgacgcc
     7561 acccgcgatc tgccgcaggg cgacttcgtc acggcccccg agctgacgcc gctgttcgcc
     7621 tgcaccctgg cgcaacaggc gcatcaagtc ctgcaggcca ccggcacccg cactgtgctg
     7681 gagttcggcg cgggcagcgg cgcgctggcc gaaggggtcc tgcgggaact cgaccgcctc
     7741 ggccaccccg tgcaatacca gatcatggaa gtatcggccg acctgcggca gcgccagcag
     7801 gcgcgcctgg ccgcctacgg cgaacgcgtg cagtggctgg acgccctgcc cgctgccttc
     7861 gagggctgcg tgctggccaa tgaagtgctc gacgccatgc cggtctcgct gtttgcctgg
     7921 aatgacgccg gccgcgtgat gcagcgcggc gtggcgctgg acgcaacggg cggcttcatc
     7981 tgggaagacc ggaccgcccc cgccgtgctg gcgaaggccg tcgccgcccg catgccgccc
     8041 ctgcccggct atgtgtccga gatcaacctg caggccgagg cctggatgcg ggaaatgggc
     8101 ggctggctcg cgcgcggcgc ggcgctcatc atcgactacg gctttcccca gcgcgagtac
     8161 taccacccgc aacgcctggg cggcaccctc atgtgccatc tgcgccacca tgcccacgcc
     8221 gatcccttcg tcgccccggg cctgcaggac atcaccgccc acgtcgattt caccgccttg
     8281 gccgacgccg ccctggacgg cggactggag gtgctgggct atgcctcgca ggcgcacttc
     8341 ctgatcaatg ccgggctgct gcagattctg ggccggcagg ataccggcga cgtccaggcc
     8401 tacgcgcagc tcgccgcgcc ggtgcagaag ctgctgtccg aggccgagat gggcgaactc
     8461 ttcaaggtgc tggccgtcgg ccgcgcggtg gacgcgtcgc tcatcggctt tgcccgcggc
     8521 gaccgccgca attcgcttta agcctcgggg ccttgccgca aggcgtccag gcgtgcctgg
     8581 cgcagcgcgg gtttgatgcg ggccggatcg cccgcgcacg cctgcgcgat ggcgcctgcg
     8641 tcgatgccgc gcacggcatc cagcctgcgc tgccaggcgg cctcatccac cggcgcgccg
     8701 accacggccg ccgcccgcag caatgccatg aaacgttccg gcttgcgcaa ggcatcgcac
     8761 tgttcgatca gggccaggcg gccctgcgca tccgtcgcgc ccggcaagcc atccaccaga
     8821 cccggcagca ggcgggcctg gtccgcgcaa tcggccggca cgcgcaatcg cttgcccaag
     8881 gcctcgcgcc gctcggacaa gcggctcagc aaggcatagc gcgaaggcag gggcaggccc
     8941 gcggcggccg cgcgatccac ctccgcgccg acctgcgccg ccgcctgcaa ctcgggcatg
     9001 acccgggcca gcgcgcccga ctgcgccagc acgtccagca tgcgcgaagg cagcgccgac
     9061 atcagcccgc gcgacacttc cttccacacc cgctcgggca ccagggcatc ggcctcgccc
     9121 gccccgacca tctggcggca caaggccatg gtctcgccgg ccacgccgaa gtcggtaaag
     9181 cgcgccatga agcgcgccag gcgcaaaatg cgcaccggat cttcggcgaa ggcctcgccc
     9241 acgtggcgga acacccgcgc ccgcagatcg gccacgccgt cgagcggatc ggccagcacg
     9301 ccctcgcgcg agcgggccat ggcattgacc gtgaagtcgc ggcgccgcag gtcctgctcg
     9361 agcgtgacgt ccgcgccggt ataaaaggtg aagcccttgt agccgcggcc cgacttgcgc
     9421 tcggtgcgcg ccagcgcgta ttcctccttg gtgaccggat gcaggaacac ggggaaatcg
     9481 ccgcccaccg gcgtgaagcc gcggcgcgcc atgtcctcgg gcgtggcgcc caccacgacc
     9541 cagtcgcggt cgccggccgg caggcccagc agcgcgtcgc gcaccgcgcc gcccaccaca
     9601 taggcctgca ggccggcggt ggcggggtcg gcggcggact cgggggccgg atcgatgggg
     9661 ttcatgaaga cagggctccg atacggctgc cccgcggcag ccttgccgcc attattgcgt
     9721 atcggagttg gccagcacct gcgactggcc ggggtcgacc aggcccaggc gcgccttgag
     9781 cgattgcggc cggccggtga agatcaccgc gtaataggtg gcgttggcca tgactttctg
     9841 cacgtagtcg cgcgtttcgt tgaaagggat ggtctcggcg aagatggcgc cctcgacgcc
     9901 gtgcgacagc gcggcgcgcc agttgcgcgg ccggccgggc ccggcgttgt agccggcgga
     9961 agccagcatc tgcgaaccgt ccacggcctg cagcacgatg ttcaggtact gcgtgcccag
    10021 ctcggtgttg acatcgaaat cgtgcacgcg ggtcgggtcg aaatcggtca tgccgatctt
    10081 cttggccacc cacttggccg tggccggcat gatctgcatc aggcccgcgg cgcccacata
    10141 cgaatgcgcc tgcgtcacga agcgcgactc ctggcggatc agcccgtaca cccaggccgg
    10201 gtccagcgag atcgcctgcg ccttggcggc caccttgccg tcgaagggcg cgatatagcg
    10261 ctgggtgaag tcgaattcgt gcgcggtgag ctcggacgtg ttgaccaccc ggtcgtagat
    10321 gtcttcggcg cgggccagct cggccgcggc cagcagttgc cggtcggtca tgccgcgcag
    10381 cgcgaagttc cattccggca cggcttcggc gcgccagccc aggcggaaca gctgcacggc
    10441 gcgcaccagg ccgggattgc ggcgcgcctc ggccatttcg gccgaagtca ccggcgccgc
    10501 ttcgggcggc aggacgatgg ggcggcccag ctcttcggaa gccagctggc cgtagaaatt
    10561 gaactggtcg gcaatgccgg cataggcctg gctggcggcg tccgcctggc ccgacgcggc
    10621 cagtccgcgg gccttccagt acacccagcc cggctcggcc tgctgctcgg gcggcatctg
    10681 ggctatgcag gccagcaccc atttccagtc gatcctggcg gcgcgcaagg cgctgcgcac
    10741 gcgccaggcc tggccggtct tgctcaggcg cacgtcgccc gcctcgcggt accactgctc
    10801 ggcgcggtcg tcctgggcgt aggcggccgc cagcgcatac tggctgcgca cccaggccgc
    10861 ctcgcccttg gccatgtagc gctcccactc gttgcggaaa tacgcgtcgg ccgtattgag
    10921 gtcctggcgc gccaggcggg ccagcgcgat cgacagcagg gccttctcgt tgcggcccac
    10981 caccgacttg ctctgccgcg ccagccactt cagcgggtct ttcagcagca ccgccagctt
    11041 cttgcggtcg ttgttttcga agatgtagcc ggccagctgc gtggccgcgg gaatgcggtt
    11101 gtcctcgatg gcgtcgcgca gcaggggcat gatctggtcc cagcccagca cgccgtcggc
    11161 caccagctgg tcgtacagct tccagcagct gcgcaccggc tggaacaccg ccagcgcctg
    11221 ctcggccgtg gcgcgccggc ccgtcatgtg ctgggcgtcg agccgggcgc aggccacggc
    11281 cgggccgcgg ttgataggct cgcccaggtc ctgcaccgcc tggaaatcgc cgcgcgccgc
    11341 cgccatgagg atcagatccg cgcgcagctt gtcggccagg tagctctggg gatgggcatc
    11401 caggaattgc tgcgcgtcgt cgaacggatg cgagcccggc agcgggaagg ccagcttgta
    11461 gcgcagcagc cagtattcgg catagatgcc cagcgggtcg tcgcgcgcct gcgcggcggc
    11521 ggcctgcgcc agggcgcccc actgcggccg gccttgcgca tcgtgggcgt cgatcagggc
    11581 ctggcgggca ggagtgtcgg gagaaggcgg caggccggcc tgcaccgggc gaggctggat
    11641 cgcggcatgg aaaaccggca ggggccccgt cgccagcgag gcaagcgcag gggcctgggc
    11701 cgcggcgggc gccccggtgc tttgctgggc gtcgaccgac gagcaggctg caatcaccat
    11761 ggccagcatc ggcagccaga gacgtatgcg cgagaccacc caggctgcgg atggccggac
    11821 cggcgaagcg gatttctgat aacgtgccgt ttcgggatcc tgcgtcattg cccttccttg
    11881 atgcgggtgc agcgtgcgga tgttgcgtat gcggccgtcg ggccgatgcg caatgaatcg
    11941 tcacaactgg tcatgccaaa tcaaacttcc gcacagaata cagcagccgc gctgcgcgcg
    12001 cgattgaaac aagcgcgcgc cgacctgccc gacgatgagc gcgaccgcgg cgccctgctg
    12061 atgcgcggcc gcctgttcag ctggctcaac gcggcccgcg atgcgctggc cccgtcggga
    12121 cgcacgattt cctcggtggc ggccttctgg cccctggccg gcgagcccga cctgcggccc
    12181 ctgttggcgc agtggtcgga ccatggcgtg caggtctgcc tgccctgcgt gacaggcccc
    12241 gggcagccgt tggaattccg caactggacg cccgcggcgc ccatggccgc cggcgcctac
    12301 ggcatcgccg aaccggccgc gggcccggcc tgcgtgccgg acgtgatcct ggttcccacg
    12361 ctgggctata ccgaccgcgc cgatcgcctg ggctatggcg gcggctacta cgaccgcacg
    12421 ctggccgcgc tggacgaagc cggccatggc tgcatcgcca tcggcatcgc ctggaccgaa
    12481 ggccggctgc ccgaggacta cgaacccgcc gcgcacgacc ggcgcctgga tgccatcctc
    12541 acccccgacg gctggctgcc gaacgcgccg ctcggcgcgg cccccgattc ggccaggtcg
    12601 cgcatcatgc gattcatcct cggttaatcc cctagccatc tagcagcact atcatggtgc
    12661 cggatgccct catacgaggc gtccgtcgcc gatttttccg tttgaatcgc cagcacgcac
    12721 tggaaccgtg ccgaagccag ccaattcaga gctggcatga cgcttgctcg taggtgtaag
    12781 ccctgtttga cgcacgagag cggctcactc aagctgccaa tacagtgcgc atgtgaagga
    12841 ggattcatgc acgatgctgc accccgtccc gccgcggctt acgacgaaat gtatgacgcg
    12901 cagggccaag tacgtcccca gtaccgggcg tacgcagact ggctggacag ccagtcttcc
    12961 gatgccatgg cagcccgcca gatcgaggcc gatctcagtt tccgccgggt aggcatcaca
    13021 ttttcagtgg ccggcgacgc cgccggcacc gaacggctca ttccgttcga cctgatcccc
    13081 cgcatcatcc ccgccaacga ctggcaacgc ctcgaggccg gcctcaagca gcgcgtgcgc
    13141 gcgctgaaca tgttcatcca cgacatctac cacgggcacg acatcgtgca cgcgggcgtg
    13201 gtcccggccg agcatgtgtt cctcaacgcg cagtaccgcc ccgagatgca ggacatcgac
    13261 gtggccgagg acatctacag ccacatcgcc ggcatcgatc tcgtgcgcgc cggccagggc
    13321 gagttctacg tgctcgagga caacctgcgc gtgccctcgg gcgtgtcgta catgctcgaa
    13381 gaccgcaaga tgtccatgcg cttgatgccc gacgtgttct ccaaggtcaa ggtcgcgccc
    13441 gtcgcccact accccgacct gctgctcgac aacctgcgcg aggtcgcccc gcgcagcatc
    13501 gacgacccca ccgtggtggt gctgacgccg ggcatgtaca actcggccta tttcgagcat
    13561 gccttcctgg cccagcagat gggcgtggag ctggtcgagg gccaggacct tttcgtcgaa
    13621 cagaacaccg tctacatgcg caccacgcac ggcccgcagc gggtcgacgt gatctaccgg
    13681 cggctggacg acgacttcct cgatccgctg tccttccgcg ccgactcggt gctgggcgtg
    13741 ccgggcctgc tgtcggtgta ccgcgccggg cgcgtcacgc tggccaacgc catcggcacc
    13801 ggcatcgccg acgacaaggc cacctacctg tacgtgcccg acatgatcaa gttctacctc
    13861 tcggaagagc cgatcctgtc gaacgtgccc acctggcagt gcgccaagcc cgccgagctc
    13921 tcgcacgtgc tggccaatat gcacgagctg gtcgtcaagg aagtgcacgg cgccggcggc
    13981 tacggcatgc tggtgggccc cacctcgagc aaggcgcagg tcgaggactt caagacgcgc
    14041 gtcaaggcca accccgccaa ctacatcgcc cagcccacgc tggccctgtc caccgtgccc
    14101 acttacgtcg agtccggcgt ggcgccgcgc cacgtcgacc tgcgccccta cgtgctgtgc
    14161 ggcaaggaca tccgcaccgt gcccggcggc ctgtgccgcg tggcgctggt cgagggctcg
    14221 ctggtggtca acagcagcca gggcggcggc accaaggaca cctgggtact ggaggattga
    14281 gatgttaagc cgcaccgccg acaacctctt ctggatgtgc cgccacatcg agcgcgccga
    14341 gaacaccgcg cgcatgctcg acgtgaacct gcagatgtcg ctcctgccgc aagacctgca
    14401 gacgctggag aactcctggc gcgccgtgct gcgcatctcc gagctgcagg gcggctatga
    14461 cgcgcgctat cccggcatca cgccgcagga cgtgctgcac tacatggtgc gcgaggccga
    14521 caacccctcg tccatctacg cgtgcctgcg cggcgcgcgc gaaaacgccc gcgccgtgcg
    14581 cggcagcctc accaccgagg tctgggaaac ctacaacagc acctggctgg gcctgctgca
    14641 gcatctcgac aacgggctgc ccgagcgcaa ccccggcgag ttcttcgaat gggtgaagtt
    14701 ccgcgcgcac ctggcgcgcg gcgtcaccgt cgccaccatg cagaccgacg aggcgctgta
    14761 cttcctgcgc ctgggcatgc atctcgaacg cgccgacaac accgcgcgca tgctcgacgt
    14821 gaaattccac gagcgcgacg acgacatgcc cgccggccgc gccgccggcg ccgatgccga
    14881 caatggcatg tacagcgagt tctaccgctg gtcggccctg ctgtcgtcca tgtcgggcat
    14941 ggagatctac cgcaagatct accgcgacgt gatcgcgccc gacc
//
LOCUS       DASWPO010000143        14905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_210769, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000143 DASWPO010000000
VERSION     DASWPO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210769"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VGC69_07905"
     CDS             <1..264
                     /locus_tag="VGC69_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0295242.1"
                     /translation="AFFGTALCSHLIDKQVDTVIVAGCTTSGCVRGTVVDAFSLNFKV
                     AVAQECVYDRGATSHKVNLFDMSQKYADVLQVNDLIARLRAIA"
     gene            299..1270
                     /locus_tag="VGC69_07910"
     CDS             299..1270
                     /locus_tag="VGC69_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295243.1"
                     /translation="MHLFKNLTRLLAAAALCGAAAAHAQYPDKPIRLVVPFPPGAGTD
                     ATARLIANELSPLLGQPVIVENRVGAGGSIGAQWVADAEPDGYTLFFSTTGALVINPH
                     LYKHLRYDGVKSFTPIGMVASSANVLVVNPQVPAHSVTELIQLAKSEPGKLTYGSSGI
                     GSSSHLAVVMLESLSGTKFTHIPYKGSAPAVTDLVGGRIDLMIDNIPSHVELVKAGKE
                     RALGVSGTNPSPLFPGLPTIAQAGVPGYDVTIWYGILGPAGMPGDVVQKLSTLIRQAV
                     TKPGMAEKLQAIGSDPLVQSPTEFASYIKSEDQKWARIVLESGATAD"
     gene            complement(1282..2418)
                     /locus_tag="VGC69_07915"
     CDS             complement(1282..2418)
                     /locus_tag="VGC69_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0295244.1"
                     /translation="MIARNRKQQGRRRAQRGFALVELAVALMIGTLLLVWGSAAVMRR
                     ADDAAAQATGAWLLEIRQASQRMLERHVDILAEGGVPAGADGQPLYADARAPTLAEFK
                     AQGLLPQGFSESAPTGGTALIRLMPPSACPGEACRVDMLVYASQALTDAYGAPDMMRM
                     AVLTDAAGGYGGHASAGSAGRLRGKIFDFPNPYAPGAEALPAGTPVLWAGVDVASTSQ
                     SRYVRRFDDRDPQLKGGLTVAGGINGEGLVTGWRLQAREFLQVSGWASPGSGCESDGL
                     VGRTGAGLLVSCQSGVWTASPPTQTVVQYFSGPGGGAFWANGTVSCPGGYKVLSGGAQ
                     CIAGVASTLSYSNPQGDWGWYASCETGRTGAETDIQVYAVCMMR"
     gene            complement(2431..3042)
                     /locus_tag="VGC69_07920"
     CDS             complement(2431..3042)
                     /locus_tag="VGC69_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HEY0295245.1"
                     /translation="MAFVVPSSASRQRGFSLIEVSIVAAIILLAAIIGIPAIGNYVIE
                     NKVPEVGRELQRFVARTKANSQGAGAMPYTDIDTGTLANAVRDSSVLSVNGTGAAAVV
                     AHGLGGAGTTGNGVVTVEPVVFASGGAGSGFAITLNRVSHAACPSLASVMQRSAEIIA
                     VQGQGGSVVVKDSTAAPPLVYMAPRAEAQCAEGDNNTFIFTVR"
     gene            complement(3102..4265)
                     /locus_tag="VGC69_07925"
     CDS             complement(3102..4265)
                     /locus_tag="VGC69_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295246.1"
                     /translation="MRLANSVADSARLLAQAETWSRWMAWIQARLDAWRFRTLRADYY
                     GYLAEMLRALQGRKTLLDHFEDDARRYGPASVRGRLSRHWARRYQETGGDLAAAWTGV
                     LPADECLLIAAGQQAGGEALVAVLRDLAHVVQLVRTVRDELLATTAAGVAAMLVAFAL
                     LCAVPYFTVPRLQHVFQGLPGEYLGPVARALYALADGVRDSFVVWIFGLPAGVALILW
                     SLPNLTGRLRGRLDRFLVWRLYRDFHAIRFLAMLCVLLRRRGNIDTRLREALSGLAWN
                     ARPWLAAHVGAMLARIDAGIVGADSLDTGLLDRELWWFLSDMAGAHGLVGGLQQARLR
                     IESHAAARVRRQAQALRWLCLLGATGTVISLSLWHYAAIDELRRALMQFHASG"
     gene            complement(4262..5995)
                     /locus_tag="VGC69_07930"
     CDS             complement(4262..5995)
                     /locus_tag="VGC69_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0295247.1"
                     /translation="MSEPRVADSAGSRPYEAAPVARGRPVLESPDDLAALVPRFVRSL
                     GAEFDLAALSGRVCPVLFEDGRAALFALADYADGDQADELERMLQRRGLRLADPARYV
                     VVSSLLLTVARDPLHGALAGHAEMPSQARRSALASMFAEFVRWGVQAGASDLHINVNE
                     RGGASQVRYTINGAYVAPDCFEGIAASTLREVLAVAWMDVRGGNGAVFDPRIEQQGRI
                     AMQVDGTPFMLRWASLAADAGPSACLRLLRLDTPDGGATLAQLGYLPSQVRMLEGARE
                     REGGAIVIAGIVGSGKSTTLATLMRGIDPARKVITLEDPVEYLIGNALQNTVGRSLDA
                     EVQHPFDAKLRTIKRSAMNDLLIGEVRDHETGRAFMDLAGSGASVYTTTHTGSALMIP
                     ERLASDFIGVSRDFLAAPGVLKLLVYQMLLPRLCTACALPFEVLWRDSGRPGEGLAWR
                     NWAVEFGRVFSADPRRLRVRNPAGCAACNRPGLERMALSGHDGRTVAAEMFEPADDAV
                     LLDCIRRRDNPSLMRHLASLARSPADDVDMSGKSVSLCAVYKALRGEIDARMLARQFG
                     LCGWARRRTAP"
     gene            complement(5988..6425)
                     /locus_tag="VGC69_07935"
     CDS             complement(5988..6425)
                     /locus_tag="VGC69_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295248.1"
                     /translation="MDAAPAAAVESPWPEADTVLDLLRADAQAAVAGKQLERAQDWLA
                     MPASVASLHAPAASRADPSGNDRDRLDVLAIYGVGQALHADIAINGELWRYRQGRRWP
                     QGIDDAAGEPRYSLVRIEQPCVRLRWQEAQRTVCLHRREGGDE"
     gene            complement(6499..7779)
                     /gene="pilO2"
                     /locus_tag="VGC69_07940"
     CDS             complement(6499..7779)
                     /gene="pilO2"
                     /locus_tag="VGC69_07940"
                     /inference="COORDINATES: protein motif:HMM:NF018562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HEY0295249.1"
                     /translation="MKQVSSHAFMLRRDGAGQTLVFGLTWFALVGSHIAAMARSRARQ
                     LKATHYAAGGLRAAAGGCVRLDRGARRGELHAAAQAYAQLHAEGAACSVVALPDGRYW
                     LVATQDGAVMSRGDHIHPTREAAEQALRVLQAQRPALRLIDGESLLDRMMQSLDAGSR
                     LLPVDTRWSSLPWPVRACVLGAALVLLWPPVRDRWRAYHAPAPVLPDLVASRQAAFDA
                     WRGGIRIHTPADLKRLTESFHRIPLALRGWTLKRIACEPQGRDWRCAAHYERAWSQAT
                     FDALAAAAPQGLQAEFETLDRSVLRWQVPGASTTLAAVSLPRRPAQAFLGNLQRIHPA
                     FSEIRLGLPVEPAVPSIQAGQAGPVAVKPDLPGLRTRTLSVTGPLRSFGVLALQSTVA
                     SWSSFTLRVDAGRPSALAGSVLLAQLQGTVYELH"
     gene            complement(7790..9379)
                     /locus_tag="VGC69_07945"
     CDS             complement(7790..9379)
                     /locus_tag="VGC69_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295250.1"
                     /translation="MKRGHMAGVALLTSMLGACALPQKMEALRGRADAAQQSLDAQQR
                     RFKQGLADVEARKAAQDVARPWLAGPAQPLAREVLLPPALRANVDTTLLFAGQADLPG
                     IAERLYRATGIAVRVHPDALLPHDLFLPRLAEKAPLAVAGATRVEVHAGPRPLADTLD
                     ALAAQLYLHWRYYQGAIEFYRTQTRVFDVRALTLASKVDARLGRAANPQSGGFESSSS
                     TALSSGEHLALQAVRSRLEPFLTRAGVIAEVESGGTALVVTDTPDVLDRIGSFIEREN
                     RVLTRRVRLVFEEITLAAHDTSEAGLDWSAIYASLRGTAESAMAAGAGATAGTLQAGI
                     AAGPFQGSRAIVSALSERGAVLRHASVPLQTLNRRPVTHAVRSTFTYIDQVQSVALPS
                     ASSGITAALPSVSISQKQETVGTLLTLVPDVQDDGSILLSVSYDNTVAQPLKSISFGQ
                     EGNQLQVQQLNVDGNGMVQQVELRPGVPVILSGFDRREDEFDRRRLTPDSPLLTGGLD
                     RGARDRLTTVVLITAQVEDGL"
     gene            complement(9376..10245)
                     /locus_tag="VGC69_07950"
     CDS             complement(9376..10245)
                     /locus_tag="VGC69_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HEY0295251.1"
                     /translation="MVRIICLLSLAWLAGCAAWGGMAFEQGAAGQYDFNWRLSGDPAV
                     APLQVFGTGRQIWLQFPPGQEVPAIFASTPQGDLPLAYRRQDPYVVIDGAWQALTLRG
                     GRYVARASRQDVPVASSREMPVAAANPPAPAGGPAKAALTEHVPLVHVPLVGDTTATP
                     VVPVQPPRDSSGRPSASAAGEPATAHPVLAPMVLHAGPPDATLRGVLARWAAASGWTF
                     EPQHWAVDVDIPLAGAAEFPGDFKQAVRALLASTELSERPVQPCFYANQVLRVVAYTQ
                     ACDRSAAPAGAAS"
     gene            complement(10424..12943)
                     /gene="rnr"
                     /locus_tag="VGC69_07955"
     CDS             complement(10424..12943)
                     /gene="rnr"
                     /locus_tag="VGC69_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY0295252.1"
                     /translation="MNAKAQAGAAQQDIPAEGGPPANAPRAAKKPKPKVTPHTPPDFD
                     PDVPSREAILNALRALTTPLSPDELAQHMNVEREATRVGFDRRLSAMERDGQLMPNRK
                     GLLLLANKLDFVAGKVQGHRDGFGFLLRDDGGPDIFLAPREMLKVLHGDRVLVKPSGD
                     YRGKPEGIIVEVIERRTNKLVGRFLHEHGLSIVVPEDQRIKHDILIPPGDTNGAQHGQ
                     VVSVQIIEQPTRHTQPLGRVYEVLGEIDDPGMEIEIAVRKFDVPVDFSEEANKQASRL
                     PDVVRKTDLKGRVDLRDVPLITIDGEDARDFDDAVYCEPVELGTSQRKRPGWRLLVAI
                     ADVSHYVRPGEPLDDDALERGTSVYFPRRVIPMLPESLSNGLCSLNPSVDRLVLVCDM
                     VIPANGAKAGTVTAYQFYNAVMHSHARTTYTNVWAALQQPGGPAASALQSVLPQIQNL
                     HELYLLLAESRKKRGAIDFETIETKIVCNQLGRIEQIVGVVRNDAHKLIEECMLAANT
                     CAADFMTRSKHPGLYRIHEGPTPERLQSLREFLRTMGLSMGGGDEPTATDYGKFIDSV
                     RGRPDFPLLQTMCLRSMQQAIYSPDNVGHFGLSYPGYSHFTSPIRRYPDLLTHRVIKA
                     LLEGQRYVPQLEGQPVIKGESQSEHEHAVWEKLGLILSATERRADEASRDVEAWLKCW
                     FVKERVGEDFSGTVTGVASFGIFVTLDTLHVEGLVHVSELGSEYFQFNEALHELRGER
                     TGMRYRLTDKVQVQVARVDLEARRIEFRLVKGTSFETLRKEATRRPEEPRKIKRAAAP
                     KPQALKGQTAKQRRAEAKKASKPAQAVKQSSRAGKKSVRPR"
     gene            13326..13410
                     /locus_tag="VGC69_07960"
     tRNA            13326..13410
                     /locus_tag="VGC69_07960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13360..13362,aa:Leu,seq:cag)
     gene            13496..13572
                     /locus_tag="VGC69_07965"
     tRNA            13496..13572
                     /locus_tag="VGC69_07965"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13530..13532,aa:Val,seq:gac)
     gene            13746..14905
                     /gene="dbpA"
                     /locus_tag="VGC69_07970"
                     /pseudo
     CDS             13746..14905
                     /gene="dbpA"
                     /locus_tag="VGC69_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814910.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
BASE COUNT         2644 a         5133 c         4819 g         2309 t
ORIGIN      
        1 gcgttcttcg gcaccgccct gtgcagccac ctgatcgaca agcaagtcga caccgtcatc
       61 gtggccggct gcacgaccag cggctgcgtg cgcggcaccg tggtcgacgc cttctcgctg
      121 aacttcaagg tggccgtcgc ccaggagtgc gtctacgacc gtggcgcaac atcccacaag
      181 gtcaacctgt tcgacatgag ccagaaatac gccgacgtac tgcaggtcaa cgatctcatc
      241 gcacggctgc gggcaatcgc ctgaccggca cggccattca tacatcaagg gaaactacat
      301 gcatctgttc aagaacctga cgcgcctgct ggccgccgcc gccctgtgcg gcgcggcggc
      361 ggcgcacgcg caatatccgg acaaacccat ccgcctggtc gtgccgtttc caccgggcgc
      421 gggcaccgac gccacggcgc ggctcatcgc caacgaactc tcgccgctgc tgggccagcc
      481 cgtgatcgtg gagaaccggg tcggcgccgg cggctccatc ggcgcgcaat gggtggcgga
      541 cgccgagccg gacggataca ccctgttctt ctccaccacg ggggccttgg tcatcaatcc
      601 gcatctgtac aagcatctgc gctacgacgg cgtcaagagc ttcacgccga tcggcatggt
      661 ggcgtcctcg gccaatgtgc tggtcgtcaa cccccaggtg cccgcgcatt cggtgacgga
      721 actgatccag ctggccaagt ccgagcccgg caagctgacc tacggctcct cgggcatagg
      781 cagctccagc cacctggccg tcgtcatgct ggaatcgctg tccgggacga agttcaccca
      841 catcccgtac aagggctccg cgccggccgt gaccgacctg gtcggcggac gcatcgacct
      901 gatgatcgac aatatcccca gccacgtcga actggtcaaa gccggcaagg agcgtgccct
      961 cggcgtctcg ggcaccaatc cttcgcccct gtttccgggc ttgcccacca tcgcccaggc
     1021 cggcgtgccc ggctacgacg tgacgatctg gtacggcatc ctcggccccg caggcatgcc
     1081 cggcgacgtg gtgcagaagc tctccacgct gatacgccag gcggtgacca aacccggcat
     1141 ggccgagaag ttgcaggcca tcggctccga tcccctggtg cagtcgccga cggaattcgc
     1201 cagctacatc aagtcggaag accagaagtg ggcgcgcatc gtgcttgaat ccggggcaac
     1261 ggcggactga atgccaggcc gtcagcgcat catgcagacg gcataaacct ggatatccgt
     1321 ctccgccccc gtcctgccgg tctcgcacga ggcgtaccac ccccaatcgc cctgcggatt
     1381 ggaataggac agcgtcgaag ccactcccgc aatgcactgc gctccgccac tgagcacctt
     1441 gtagccgccc gggcaggaaa ccgtaccgtt ggcccagaac gccccgccgc cggggccgga
     1501 aaaatactgg accacggtct gcgttggcgg ggaggccgtc cagacgcccg actggcacga
     1561 caccagcaag ccggcccccg tgcgccccac caggccgtcg gactcgcagc cgctgccagg
     1621 gctggcccac cctgacacct ggaggaactc gcgggcctgc aggcgccagc ccgtgaccag
     1681 gccttccccg ttgatgccgc cggccacggt caatccgcct ttgagttggg gatccctgtc
     1741 gtcgaagcgc cgcacatacc ggctttggct ggtcgaggcc acgtccacgc cggcccaaag
     1801 caccggcgtg cccgcgggca gcgcctcggc acccggcgca taaggattgg ggaaatcgaa
     1861 aatcttgccg cgcaggcggc cggccgaacc ggcgctggca tggccgccat acccgcccgc
     1921 cgcgtcggtc aacacggcca tgcgcatcat gtccggcgcg ccataggcat cggtcagggc
     1981 ttgcgaggcg tacaccagca tatcgacccg gcaggcctcg ccagggcagg ccgaaggcgg
     2041 catcaagcgt atcagggccg tcccgcccgt cggcgcggac tcggagaaac cttgaggcag
     2101 caggccctgg gccttgaact cagccagggt cggcgcgcgc gcatccgcgt acaaaggctg
     2161 gccatcggca ccggcgggca cgccgccttc ggccagtatg tcgacatggc gctccagcat
     2221 gcgctggctt gcctgccgga tctccagcag ccaggctccc gtcgcctggg cggccgcgtc
     2281 atcggcgcgc cgcatcaccg ccgccgagcc ccagaccagc agcaacgtgc ctatcatcag
     2341 ggcgacggcc agctcgacca gggcaaagcc gcgctgcgcg cgccgccttc cctgctgttt
     2401 ccggttccgc gcgatcatgg gcacggcacc tcaacgcacc gtgaagatga aggtattgtt
     2461 gtcgccctcg gcgcattggg cttcggcgcg cggcgccata tagaccaagg gcggcgcggc
     2521 cgtgctgtcc ttgaccacga ccgaaccgcc ctgcccctgc accgcgatga tctcggcaga
     2581 acgctgcatg accgacgcca ggctcggaca ggcggcatgg ctgacacgat tgagcgtgat
     2641 ggcaaagccc gagcccgcgc cgcccgaagc aaacacgacg ggctcgacgg tgaccacgcc
     2701 attgcccgtg gttcccgccc cgcccaggcc gtgcgccacc acggcggcgg cgcccgtgcc
     2761 attgaccgac aacacgctgg aatccctcac ggcattggcc agcgtgccgg tatcgatatc
     2821 ggtataaggc atggcgcccg ccccttgcga attggccttg gtccgcgcaa cgaagcgctg
     2881 cagttcccgg ccgacctcgg gcaccttgtt ctcgatgaca tagttgccga tggccgggat
     2941 gccgatgatg gcggcaagca ggatgatggc cgcgacgatg gacacctcga tcagggaaaa
     3001 gccgcgctgc cgcgaggcgg aagacgggac gacgaaagcg atgtgcatgg aaggctcctg
     3061 gtaagtaatg ggaagcaagc gttgaaggac aggcgcctga atcagccgga ggcgtgaaac
     3121 tgcatcaagg cccgcctcag ctcgtcgatg gcggcgtagt gccaaaggga caggctgatc
     3181 acggtccccg ttgcacccaa taggcacagc cagcgcaagg cctgcgcctg cctgcgcaca
     3241 cgcgccgcgg catgggactc gatgcgcagg cgggcctgct gcaatccgcc gaccagccca
     3301 tgggcgccag ccatgtccga caagaaccac cacagttcgc ggtcgagcag ccccgtgtcc
     3361 aggctgtccg cgccgacgat gccggcgtcg atgcgagcca gcatggcccc gacatgagcg
     3421 gccagccatg gccgcgcatt ccaggccagg cctgacaggg cttcacgcag ccgggtatcg
     3481 atattgccgc gccggcgcag caatacgcac agcatggcca ggaaccggat agcgtggaaa
     3541 tcgcgatata gccgccagac aagaaagcga tccagccgcc cgcgcaggcg gccagtaaga
     3601 ttgggcaggc tccacaggat caacgccacg ccagccggca ggccgaaaat ccacacgaca
     3661 aacgagtccc ttacgccgtc ggccaaggca tacagtgcgc gggcaaccgg ccccagatac
     3721 tcgccgggca gtccctggaa tacgtgctgc aaacgcggga ccgtgaaata cggcacggcg
     3781 caaagcagcg caaacgccac gagcatggcc gccacgccgg ccgccgtcgt ggcgagcagt
     3841 tcgtcgcgaa ccgtgcggac cagctgcacc acatgagcca gatcccgcaa caccgcgacc
     3901 agcgcctcgc cgcccgcctg ctggcccgcg gcgatcaaca ggcattcgtc ggccggcaac
     3961 accccggtcc atgccgcggc cagatcgccg ccggtttcct ggtagcggcg cgcccagtgc
     4021 cgtgacaaac ggccacgcac cgatgccgga ccatagcgcc gcgcgtcgtc ctcgaaatgg
     4081 tccagcaagg tcttgcgccc ttgcaaggct cgcagcattt cggccaggta gccgtaatag
     4141 tccgcccgca aggttctgaa ccgccaggca tccaggcgcg cctggatcca tgccatccaa
     4201 cgcgaccagg tttcggcctg cgcaagcagc cgcgcgctgt ccgccacgct gttcgccaac
     4261 ctcatggcgc cgtccggcgg cgcgcccagc cgcacaggcc gaactggcga gccagcatcc
     4321 gggcatcgat ctcgccccgc aaggccttgt agacggcgca taggctgacg gacttgcccg
     4381 acatgtcgac atcgtccgcc gggctgcgcg ccaggctggc gagatggcgc atcaaagacg
     4441 gattgtcccg gcggcgaata cagtcgagca agacggcgtc atcggcgggc tcgaacatct
     4501 cggcggccac cgtgcggccg tcatgtcccg atagcgccat gcgctccagt cccgggcgat
     4561 tgcatgccgc gcaacccgct ggattgcgca cacgcaaacg gcgagggtcg gcactgaaca
     4621 cccggccgaa ctcgacggcc cagttgcgcc aggccaggcc ttcgccaggc ctgcccgagt
     4681 cccgccacaa aacctcaaag ggcaaagcgc atgcagtgca caggcgcggc agcagcatct
     4741 ggtagaccag cagcttgagc acgcctggcg cggccaggaa atcccgggag acaccgatga
     4801 aatccgaggc caaccgctcg ggaatcatca atgccgatcc ggtatgcgtc gtggtgtaga
     4861 cgctggcgcc gctgccggcc agatccatga atgccctgcc ggtctcgtga tcgcgcactt
     4921 cgccgatcag cagatcgttc atggccgagc gcttgatggt gcgcagcttg gcgtcgaacg
     4981 gatgctgtac ttcggcatcc agcgatctgc ccacggtgtt ctgcagggca ttgccgatca
     5041 ggtattcgac cgggtcttcc agggtgatga ccttgcgcgc gggatcgatg ccgcgcatca
     5101 gcgtggcaag cgtggtcgac ttgcccgacc ccacgatgcc ggcaatgacg atggcgccgc
     5161 cttcgcgttc gcgcgcgcct tcgagcatcc gcacctggga aggcaggtag cccagctgcg
     5221 ccagcgtggc gccgccgtcc ggcgtgtcca gacgcagcag gcgcaggcag gcggagggtc
     5281 cggcatcggc tgccagcgac gcccagcgca gcatgaaggg cgtgccgtcc acctgcatgg
     5341 cgatgcgccc ctgctgttcg atacgcggat cgaacacggc gccattgccg ccccggacat
     5401 ccatccaggc cacggccagc acctcgcgca gggtcgatgc cgcaatgcct tcgaagcaat
     5461 cgggcgccac ataggcgccg ttgatggtgt agcgcacctg ggaagcgccg ccccgctcat
     5521 tgacgttgat atgcaggtcg ctcgcacccg cctgaacgcc ccagcgcacg aactccgcga
     5581 acatcgaggc cagggccgaa cgccgggcct gggacggcat ctcggcatgc cctgccaagg
     5641 cgccgtggag tgggtcgcgc gccacggtca acaacaaaga ggacacaacg acatagcgcg
     5701 ccggatccgc cagccgcaag ccgcgccgct gcagcatgcg ctcgagttcg tcggcctggt
     5761 cgccatcggc gtagtccgcc agggcgaaca aggcagccct gccgtcctcg aacaagacgg
     5821 gacagacccg gccggacaag gcggcaagat cgaattccgc tcccagagaa cggacgaagc
     5881 gcgggaccaa ggccgccaga tcgtcaggcg actcgagcac gggccggccc cgcgcaacag
     5941 gcgccgcctc ataaggccgg cttccagccg agtcggcaac gcgaggctca ctcatcgcca
     6001 ccctcccgcc tgtgcaggca taccgtgcgt tgcgcttcct gccagcgcag acgcacgcag
     6061 ggctgctcga tgcgcaccag gctgtagcgc ggttcgccgg ccgcgtcatc gatgccctgc
     6121 ggccagcgtc gaccctggcg gtagcgccaa agctcgccat tgattgcgat gtcggcatgc
     6181 aaggcctggc ccacgccata gatggccagc acgtcgagcc tgtcccggtc attgccggaa
     6241 ggatcggcac gcgaggccgc gggcgcatgc agagaggcca cggatgcagg catggcaagc
     6301 cagtcctggg cgcgttccag ctgcttgccg gcgacggctg cctgcgcatc ggcgcgcagc
     6361 aggtcgagca ccgtatcggc ttccggccat ggcgactcga cggctgccgc aggggcggca
     6421 tccaccgcag gcgccggtcc gggccaagct gcgcagaggg ccgccagccc ccaggcatat
     6481 ctcctgccca ggcagaattc aatgcaattc ataaacagtc ccttgaagtt gggcgagcag
     6541 cacgctgccg gccaatgcgg aaggacggcc ggcatcgacg cgcaaggtga aagacgacca
     6601 ggaagccacg gtgctctgca gggccagcac cccgaaagag cgcagcggcc cggttaccga
     6661 aagcgtgcgg gtccgcaatc ccggcaagtc cggtttcacc gccaccggcc ccgcctggcc
     6721 cgcttgaatc gaaggcacgg caggctcgac cggcaaaccc agcctgattt cggaaaaggc
     6781 gggatggatg cgttgcagat tgccgagaaa agcctgcgcg ggccgcctgg gcaaggacac
     6841 ggcagccagc gtcgtgctcg cgcccggcac ctgccagcgc aggacgctgc ggtccagggt
     6901 ctcgaactcg gcctgcaagc cttggggcgc cgcggccgcc agcgcatcga atgtcgcttg
     6961 ggaccatgcg cgttcgtaat gcgcggcaca gcgccagtcg cgcccttgcg gttcgcacgc
     7021 gatgcgcttc aaagtccagc cgcgcaaggc caggggaatt cgatgaaatg attccgtcag
     7081 gcgcttcagg tcggcgggcg tatggatgcg aatgccgccg cgccaggcat cgaaagcagc
     7141 ctgcctggaa gccaccaggt cgggaagcac cggcgccggc gcatggtagg cgcgccagcg
     7201 gtcccggaca ggcggccaca gcaggaccaa tgccgcgccc aggacgcagg cccggacagg
     7261 ccagggcaag ctgctccagc gcgtatccac gggcagcaac cgggaaccgg cgtccaggga
     7321 ctgcatcatg cgatccagca acgattcgcc gtcgatcagg cgcagcgcgg gccgctgagc
     7381 ctgcagcacg cgcaatgcct gttccgccgc ttcgcgggtc gggtggatat gatcgccgcg
     7441 cgacatgacg gcgccatcct gcgtggcaac cagccaatac cgtccgtccg gcaaggcgac
     7501 cacgctgcag gccgcccctt ccgcatgaag ctgcgcatag gcttgggccg cggcgtgcag
     7561 ctcaccgcgc cgggcgccac ggtccaggcg cacgcacccg cctgccgccg cccgcaggcc
     7621 ccccgccgca taatgcgtgg ccttgagctg tcgagcccgg gagcgggcca tggccgcgat
     7681 atggctgccg acaagggcaa accaggtcaa cccgaacacc aaggtctgcc ccgccccgtc
     7741 ccgcctaagc atgaatgcgt ggctggatac ctgcttcatg gcgcactcct catagaccgt
     7801 cctcgacctg ggccgtgatg agcaccaccg tcgtcaatcg atccctggcc cctcgatcca
     7861 ggccacccgt caacaaagga gagtccggcg tcaggcgacg ccggtcgaac tcgtcctcgc
     7921 gccgatcgaa tcccgaaaga atgaccggga cgccgggacg cagctcgacc tgctgcacca
     7981 tgccattgcc gtccacattg agctgctgca cctgcagctg attgccttcc tggccgaagg
     8041 aaatggattt cagcggctgc gcgaccgtgt tgtcgtagga cactgacagc aggatgctgc
     8101 catcgtcctg gacgtcgggc accagggtca gcaaagtacc gaccgtctcc tgcttctggc
     8161 tgatggaaac ggacggcaag gccgcggtga tgccgctgga cgccgacggc agggccacgc
     8221 tctgcacctg gtcgatatag gtgaacgtcg agcgcacggc atgcgtgacc ggccgccgat
     8281 tgagcgtctg cagaggaacg ctggcatgcc gcagcacggc gccgcgttcg ctcaaggcgg
     8341 aaacgatggc gcgagacccc tggaacggac ccgcggcaat acccgcctgc aaggttcccg
     8401 cggttgcgcc cgcaccggcc gccatagcgc tttcagcggt gccgcgcaag ctggcataaa
     8461 tggccgacca atccagaccg gcttcggaag tgtcatgcgc ggccagggtg atctcctcga
     8521 ataccaggcg cacccgccgg gtcagcaccc ggttctcgcg ttcgatgaag gagcctatgc
     8581 gatccagcac gtcgggcgta tcggtcacca ccagcgccgt cccgccgctc tctacctcgg
     8641 cgatcacgcc cgcccgcgtc agaaaaggtt cgagcctgct gcgcacggcc tgcagggcca
     8701 ggtgctcgcc cgagctgagc gccgtgctgg aactgctttc gaaaccgccg ctctggggat
     8761 tggccgcgcg ccccaggcgg gcgtcgacct tgctggccaa ggtcaacgcg cgcacgtcga
     8821 agacacgtgt ttgcgtccgg tagaactcga tggcgccttg gtaatagcgc caatgcaggt
     8881 aaagctgcgc ggccagcgca tcgagcgtgt cggccaaggg gcgaggcccc gcatgaactt
     8941 ccacgcgcgt ggcgccggcg accgccaacg gcgccttttc ggccagccgg ggaagaaaca
     9001 agtcatgcgg cagcaaggca tcgggatgca cgcggaccgc gattcccgtg gcgcgataaa
     9061 gccgctccgc aatgccggga aggtcggcct gcccggcaaa gagcagggtg gtgtcgacat
     9121 tggcccgcaa ggccggcggc agcaggactt cacgcgccag gggctgggcc ggcccggcca
     9181 accatggcct ggccacgtcc tgcgccgcct tgcgcgcctc gacatcggcc aggccctgct
     9241 tgaagcggcg ctgctgcgca tcgagcgatt gctgcgccgc atcggcccgg ccgcgcaagg
     9301 cttccatttt ctgtggcagc gcacaagccc ccagcatgct cgtcaatagc gccacgcccg
     9361 ccatatgtcc gcgcttcatg acgcggctcc ggcaggagca gccgagcggt cgcatgcctg
     9421 ggtgtaggcc acgacccgca acacctgatt ggcatagaag caaggctgca ccggtcgttc
     9481 ggacagttcg gtggacgcca gcaatgcccg caccgcctgc ttgaaatcac ccggaaactc
     9541 cgccgcgccc gccaggggaa tatcgacatc gaccgcccag tgctgaggct cgaacgtcca
     9601 gccggaagcg gctgcccacc tggccagcac gccgcgcaag gtcgcatcgg gcggacccgc
     9661 atgcagcacc atgggggcaa gaaccggatg tgcggttgcc ggctcgcctg ccgccgatgc
     9721 cgatggcctg cctgacgagt cacgcggcgg ctggaccgga accaccggcg ttgccgtggt
     9781 atcgccgacc aaaggcacgt gcaccaaagg cacatgctcg gtcaaagccg ctttggccgg
     9841 tccgccggcc ggcgcaggcg ggttcgccgc agcgacaggc atctcccgcg aggaggccac
     9901 cggcacatcc tgccgcgagg cccgtgccac atagcggcca ccgcgcaagg tcagggcctg
     9961 ccatgccccg tcgatgacga cgtaaggatc ctggcgacga taggccagcg gcaggtcacc
    10021 ctgcggcgtg ctggcgaaaa tggccggcac ttcctggccc ggcgggaact gcagccaaat
    10081 ctgccggccc gtgccgaaca cctgcaaggg cgccacggcc gggtcgcccg acaggcgcca
    10141 gttgaaatcg tattgtcctg ccgcgccctg ctcgaacgcc atgccgcccc atgcggcaca
    10201 acccgccagc caggccaggc tcagcaaaca gatgatgcgc accatgcgaa ccccgatcga
    10261 aaatgacatc gggttgcatg gtcatccgcg cggcggattg cggctagacg gacatctgcg
    10321 aatggatgaa atagtcacgc gtgaagcgcg tgttgcatgt ctggtcgaca tccatggcaa
    10381 atctgccgtt cataaaaaag cgggcctctg cccgcttttc acatcagcgc ggccgcactg
    10441 atttctttcc tgcccgcgac gactgcttta ccgcctgggc aggcttgctg gccttcttgg
    10501 cttcggcccg gcgctgcttg gccgtctggc ccttcaaggc ctgcggcttg ggcgcggcgg
    10561 cgcgcttgat cttgcgcggt tcttcgggac ggcgcgtggc ttctttgcgc aaggtctcga
    10621 agctggtgcc cttgaccagg cggaactcga tgcggcgcgc ctccaggtcg acgcgcgcca
    10681 cctgcacctg caccttgtcg gtgaggcggt agcgcatgcc ggtgcgttcg ccgcgcagct
    10741 cgtgcaaggc ctcgttgaac tggaagtatt cgctgcccag ctcggagacg tgcaccaggc
    10801 cttcgacgtg cagggtgtcc agcgtgacga agatgccgaa gctcgccacg cccgtgacgg
    10861 tgccgctgaa gtcctcgccc acgcgctcct tgacgaacca gcacttgagc caggcctcga
    10921 cgtcgcgcga ggcttcgtcg gcgcggcgct cggtggccga gaggatgagg cccagctttt
    10981 cccagacggc gtgttcgtgc tcgctctgcg actcgccctt gatgacgggc tggccttcga
    11041 gctgcggcac gtagcgctgg ccttcgagca gcgccttgat gacgcggtgc gtgagcaggt
    11101 cgggatagcg ccggatgggc gaggtgaagt gcgaatagcc cgggtaggac aggccgaagt
    11161 ggccgacgtt gtcggggctg tagatggcct gctgcatgga gcgcaggcac atggtctgca
    11221 gcagcgggaa atcggggcgg ccgcgcaccg agtcgatgaa cttgccgtag tcggtcgcgg
    11281 tgggctcgtc gccgccgccc atcgacaggc ccatggtgcg caggaattcg cgcaacgatt
    11341 gcaagcgctc gggcgtgggg ccttcgtgga tgcggtagag gcccggatgc ttgctgcgcg
    11401 tcatgaagtc ggccgcgcag gtgttggccg ccagcatgca ttcttcgatg agcttgtgcg
    11461 cgtcgttgcg caccacgccc acgatctgct cgatgcggcc gagctggttg cagacgatct
    11521 tggtttcgat ggtctcgaaa tcgatggcgc cgcgcttctt gcgcgactcg gccagcagca
    11581 ggtagagctc gtgcaggttc tggatctgcg gcagcacgga ctgcagcgcg ctggccgcgg
    11641 ggccgccggg ctgttgcagg gccgcccaca cattggtgta ggtggtgcgc gcatgcgagt
    11701 gcatgaccgc gttgtagaac tggtaggccg tgacggtgcc ggccttggcg ccattggccg
    11761 ggatgaccat gtcgcacacc agcaccaggc ggtcgacgct cgggttgagc gaacaaagcc
    11821 cgttggacag cgactcgggc agcatgggaa tgacccggcg cgggaaatac acgctggtgc
    11881 cgcgttcgag cgcatcgtcg tccagcggct cgccgggacg cacatagtgg ctgacgtcgg
    11941 cgatggccac cagcaggcgc cagcccgggc gcttgcgctg gctcgtgccc agctccaccg
    12001 gctcgcaata caccgcgtcg tcgaagtcgc gcgcgtcttc gccgtcgatg gtgatgagcg
    12061 gcacgtcgcg caggtccacc ctgcccttca ggtccgtctt gcgcaccacg tcgggcagcc
    12121 gcgaggcctg cttgttggct tcctcggaga aatcgaccgg cacgtcgaac ttgcgcaccg
    12181 cgatctcgat ttccatgccg gggtcgtcga tctcgcccag cacctcgtac acgcggccca
    12241 ggggctgggt gtggcgcgtg ggctgctcga tgatctgcac cgacaccacc tggccatgct
    12301 gcgcgccgtt ggtatcgccg ggcgggatga ggatgtcgtg cttgatgcgc tggtcttctg
    12361 gcaccacgat ggacagcccg tgctcgtgca ggaagcggcc caccagtttg ttggtgcggc
    12421 gctcgatgac ctcgacgatg atgccttccg gcttgccgcg gtagtcgccc gaaggcttga
    12481 ccagcacgcg gtcgccgtgc agcaccttga gcatttcgcg cggggccagg aagatgtcgg
    12541 gcccgccgtc gtcgcgcagc aaaaagccga agccgtcccg atgcccctgc accttgcccg
    12601 ccacgaaatc gagcttgttg gccagcagca gcaggccctt gcggttgggc atgagctggc
    12661 cgtcgcgctc catggcggag agccgccggt cgaagcccac gcgcgtggct tcgcgctcga
    12721 cgttcatgtg ctgcgcgagc tcgtcgggcg acaacggcgt cgtgagcgcg cgcagggcgt
    12781 tgagtatggc ttcgcgcgaa gggacgtccg gatcgaaatc cggcggcgtg tgcggcgtga
    12841 ccttgggctt gggcttcttg gcggcgcgcg gcgcattggc cggcgggccg ccctccgcgg
    12901 ggatgtcctg ctgcgcggcg cccgcttggg ctttcgcgtt caccgcttca tcggctgcgc
    12961 tgtccactct cgatttttca agcgattttt caaatgcctc ttcaggcact ttttcaagcg
    13021 ttttttcgac cgttttttca gccggttttt ccaccgcctt ttcaatcttg ggctggccct
    13081 ctggcatgtt tcgcaggcca tccaggagga cggatggttt gtgtgtaccg ttgttcggtc
    13141 gttttgccaa agctgaaaat cccgtatata atgcgcagct tctgtggcca gcccaaggct
    13201 agacactgaa tagaatgtac cgctgatttg tactgatgta ccactggggg ctccaggttt
    13261 ttgactggag cacgaatgct tcgaagcgta tcacaaccca atgttcagcg tgacggacta
    13321 gcagtgccca ggtggcggaa ttggtagacg cgcatggttc aggtccatgt gccgcaaggt
    13381 gtggaggttc gagtcctctc ctgggcacca cagacaaccc ccggttggca cttcgtgcag
    13441 accaccccca aaaggggttt gcgggttcta gagcagtatc agccgaaagg cttgaaggcg
    13501 gttagctcag atggttagag cgccacgttg acatcgtgga ggtcgttggt tcgattccaa
    13561 taccgcctac cagaaccaat acaagcagtg ccgagcattg ccaagagagc cgctcaagtc
    13621 cctgacttgg cggctttttg tttatcttcc gcggtaagac catcatcgga cgcggcaaaa
    13681 aaaggtaaag gcatacactt atcggtcgat ccagactttc ccagtttttg cgggcccgcc
    13741 ttattgtgac caacacgtcc ttctcctccc tgcccctctt gcccgccttg ctggacaacc
    13801 tgcagggcct gggtttcgaa cagatgacgc ccatccaggc gcaaagcctg ccgctcatcc
    13861 tggaaggccg cgacctgatc gcgcaagcca agaccggcag cggcaagacc gcggcgttcg
    13921 gcctgggcgt gctgcagaac ctggacgtca accgtctggc cccgcaggcg ctgctggtct
    13981 gccctacccg cgaactggcc gaccaggtgg cccaggagct gcgccggctg gcccgccaga
    14041 tccccaatgt caagatactg acgctgtgcg gcggcgcgcc cgtgcgtccc caggccgaat
    14101 cgctcgcccg cggcgcgcat ctggtggtgg gcacgcccgg ccgcatccag gaccacctgg
    14161 agcgcggcag cctggacctg tccggcctga atacgctggt gctggacgag gccgaccgca
    14221 tggtggacat gggcttctac gacgacatcg tcgccatcgc ctcgcactgc ccggccaagc
    14281 gccagacctt gctgttctcg gccacctacc ccgacaacat ccgcaagctc agcgcccgct
    14341 tcctgcgcaa cccggccgaa gtcaaggtcg aggcccagca tgacgccagc cgcatcgagc
    14401 agatcttttt cgagatcgac gaaggcgcaa ggctggacgc cgtggccgcg ctgctggccc
    14461 acttccgccc ggcatccacg ctggcgttct gcaacaccaa gatccgcagc cacgacctgg
    14521 tcgaacgcct gcggtccgag ggcatcagcg cccaggcgct caacggcgac ctggagcagc
    14581 gcgagcgcga cgaaatcctg atccagttcg ccaatcagag ctgcccggtg ctggtcgcca
    14641 ccgacgtagc cgcacgcggc ctggacatcc agaacctggg cgccgtgatc aatgtggacg
    14701 tgaccaagga caccgaagtc cacgtgcacc gcatcggccg cagcggccgc ggcgaccaga
    14761 agggcctggc gctcagcctg tgctcgcccg actaaatgcg ctgggccaat ctggtcgagc
    14821 aataccaggg cgcgccgctg aagtggggcg acatcaagtc cctgaagccc cgcgccgacc
    14881 gccccctgcg cgcgccgatg gtcac
//
LOCUS       DASWPO010000144         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2531807, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000144 DASWPO010000000
VERSION     DASWPO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531807"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGC69_07975"
     CDS             <1..311
                     /locus_tag="VGC69_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0295253.1"
                     /translation="FRYSALTFNGHRIHYDQAYARNEEGYPDLVVHGPLTATLLQQFA
                     LEQGGGRALARFEFRGVSPLFVSRGFRLEGRAAGDGELALWARGPDGELAMSATAAFR
                     "
     gene            381..1580
                     /locus_tag="VGC69_07980"
     CDS             381..1580
                     /locus_tag="VGC69_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0295254.1"
                     /translation="MNTKSNVGDAPAEGRITDEAVAAARAMIGLQLRPEGPYLQDATA
                     DTLRNWCNGIGDLNPLYRDVGYGTGTRYGTQLGHPMFPMAFGWLGRTRWGLPGVHGFY
                     AGNDWELFRHVRPGDRISAIERVVGVEEKESKFSGRLVLQYVEASYYNQRGELVARAL
                     GTCTRHERKAARDAGKYKEIEQHEYSDEERDRIDQMVLDEPKNIRGANVLYWEDVQEG
                     EELPTIARGPLSLMDTMGFLVGCGRGHTHGVVLQAAVKHPGHFFRNPEAGGGVEYTGI
                     GHHRESVAKEVGVPGTYDYGPQRSSWMCSLVTNWMGDAGVLKRVRTEMRRFNIMGDTT
                     FCKGKVIRKYVKDKAGLVDIEIAAVNQRGEVTTPGLATVALPSRDVQLPVFLDGSGVD
                     LELPVTR"
     gene            1609..1961
                     /locus_tag="VGC69_07985"
                     /pseudo
     CDS             1609..1961
                     /locus_tag="VGC69_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
BASE COUNT          327 a          636 c          692 g          306 t
ORIGIN      
        1 tgttccgcta ttccgcgctg accttcaacg gtcaccgtat ccactatgac caggcctatg
       61 cgcgcaacga agagggctac ccggacctcg tcgtgcacgg tccgctgacc gcgaccttgc
      121 tgcagcagtt cgccctggag cagggcgggg gcagggcgct tgcgcgcttc gagtttcgcg
      181 gggtcagccc gctgttcgtg tcgcgcggtt tccgcctcga ggggcgcgca gccggggacg
      241 gcgaattggc cctgtgggcc agggggccgg acggcgagct ggcgatgtcc gccacggcgg
      301 ccttccgcta gccggccgca tgcctacaaa cctattgaac aagtcttgac gaccaaggag
      361 agcaatcaat gagcgcagtc atgaacacca aatccaatgt gggcgatgcg cccgccgaag
      421 gccgcatcac cgacgaggcc gtcgccgccg cccgggcgat gatcggcctg caattgcgtc
      481 ccgaaggacc gtacctgcag gacgcgacgg ccgatacgct gcgcaattgg tgcaacggta
      541 tcggcgacct gaatccgttg tatcgcgacg tgggctatgg gacgggcacg cgctacggca
      601 cgcagctcgg ccatcccatg tttcccatgg cgttcggctg gctgggccgc acgcgctggg
      661 gcttgcccgg cgtgcacggc ttctatgccg gcaacgactg ggagcttttc cgccatgtcc
      721 gtccgggcga ccgcatcagc gcgatcgaac gcgtggtcgg cgtcgaggaa aaggaaagca
      781 agttctcggg ccggctggtg ctgcagtacg tggaggccag ctattacaac cagcgcggcg
      841 agctggtggc gcgcgcgctg ggcacctgca cgcgccacga gcgcaaggcc gcgcgcgacg
      901 ccggcaagta caaggaaatc gagcagcacg agtattccga cgaggagcgc gatcgcatcg
      961 accagatggt gctggacgag cccaagaaca tccgcggcgc caatgtcctc tactgggaag
     1021 acgtgcagga aggcgaagag ctgccgacca tcgcgcgcgg ccccctgtcg ctgatggaca
     1081 ccatgggctt tctggtcggc tgcggacgcg ggcacacgca cggcgtggtg ctgcaggcgg
     1141 ccgtcaagca cccgggccat ttcttccgca acccggaagc gggcggcggc gtggaataca
     1201 ccggcatcgg ccatcatcgc gagtcggtcg ccaaggaagt cggcgtgccg ggcacctacg
     1261 actacggccc gcagcggtct tcctggatgt gctcgctggt gaccaactgg atgggcgacg
     1321 cgggggtcct caagcgggtg cgcaccgaga tgcggcgttt caacatcatg ggcgacacca
     1381 cgttctgcaa gggcaaggtg atccgcaagt acgtgaagga caaggccggg ctggtggaca
     1441 tcgaaatcgc ggccgtcaat cagcgcggcg aggtcaccac gccggggctg gccacggtgg
     1501 ccctgccttc gcgcgacgtc cagctgccgg tcttcctgga tggctcgggc gtggatctcg
     1561 aactgcccgt gacccgctga atccgccggc cgaatgggga gtaccgacat gtctgcgaag
     1621 gattccgcgc aagccctcgc gccgcttgct ggctgccggg tggtggaacg ctcgcgcacg
     1681 gtcgcggccg cttatgcggg caggctgctg gccgcgatgg gcgcgcaagt cgtgatgctc
     1741 gagcccgcgg cgggctcgcc gctgcgcaag gccgcgcctt tgctgcagga aggcggataa
     1801 agcgcgctct tcgcctatct cgcgatgggc aagcgcagcc tggcgtgcga tccggcgtcg
     1861 gcggcgggcc gcgccttgct cgaccgcgaa ctggcgcagg ccgatctgtt catcgacgac
     1921 acgccggtgc gggaccgcga gcgcctgggc ctggatccgc a
//
LOCUS       DASWPO010000145         8517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_120075, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000145 DASWPO010000000
VERSION     DASWPO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120075"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            910..1437
                     /locus_tag="VGC69_07990"
     CDS             910..1437
                     /locus_tag="VGC69_07990"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY0295255.1"
                     /translation="MRLIPLILLALAYGLANAQDAPAADAASAREADIARIIDGIISF
                     TQWPAASGKVRLCLTTPVIHADALEAELSGSTQLADVQDLAPDDFRLDSDCDVVYIEQ
                     VDDANRAHLFQRLVGRPVLTIAGVASGCLMGSLFCLSGTGVPATFSANLDTIARSGLR
                     INPKVLFLARPHQAK"
     gene            1434..2687
                     /locus_tag="VGC69_07995"
     CDS             1434..2687
                     /locus_tag="VGC69_07995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0295256.1"
                     /translation="MTNRPASNNSPTQPRTLSQVLLRAHVGSALAAMCFVGLALMAAG
                     LLQLRSTVNQNLELVARSMAFAAEAAVVFRDGPATWSAIDEIASMASVESVAVTDMQG
                     QTLAVWRKQRANTSIAHWFQNLDFLEQSVRLPVLSHGRRVGAIRVQSSSDSIWGFLVT
                     GLLWILACVIASIVMTAYVTQRTLHSIVHPLRQFTEVTRRVRNSRSFGERVPRADLVE
                     LNDLANDFNSLLSELEHREAGAQGEYRALEHQATHDSLTGLANRTAFEIRLRHSILRA
                     QAKGRSFALLFIDGDNFKLINDNFGHAAGDLVLREVGRRISEQLRDNDDVARFGGDEF
                     AVILDPVKDKGSAERVAGAIAAAMAVPIALESGQTLVASVSVGMALYPQDATDELGLL
                     EHADATMYARKASRGDAGTHTQPAV"
     gene            2707..3216
                     /locus_tag="VGC69_08000"
     CDS             2707..3216
                     /locus_tag="VGC69_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0295257.1"
                     /translation="MIDTTAKPLWIRKLLIACMVAGTVGMAGCQSLKHGLTAEQIATL
                     KQQGFVETSQGWTLDQSATVFFATNDATLSDVGARSIDGVAKALRGVGLVHLKVVGYT
                     DSTGTDAYNDTLSKRRAEAVAGEFVNDGFPSDGLETVGMGKRYPVADNSTAQGRAQNR
                     HVAIIVIAD"
     gene            complement(3228..3434)
                     /locus_tag="VGC69_08005"
     CDS             complement(3228..3434)
                     /locus_tag="VGC69_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295258.1"
                     /translation="MALTDAVVRQAKITGKDYPLNDSDGLLLFVTARRREVPFSDREH
                     GLASARERRNLVEHIRKRGPEIAP"
     gene            3691..3963
                     /locus_tag="VGC69_08010"
     CDS             3691..3963
                     /locus_tag="VGC69_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY0295259.1"
                     /translation="MITEVSAVNFRQNLGEMINQVQYRNDSIVINKDGKPVAALVDAD
                     LFARIRRMQERFDALSRRISDGYAGVPEEEGLAEIEAAVAAERKAR"
     gene            complement(4504..4579)
                     /locus_tag="VGC69_08015"
     tRNA            complement(4504..4579)
                     /locus_tag="VGC69_08015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4544..4546),aa:Gly,seq:gcc)
     gene            4704..5753
                     /gene="murB"
                     /locus_tag="VGC69_08020"
     CDS             4704..5753
                     /gene="murB"
                     /locus_tag="VGC69_08020"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY0295260.1"
                     /translation="MSNQPVPNPQALDLFPEACDLASRNTLGLASRAEACVLLAHRSQ
                     LDALSGLARQYASLLILGGGSNLVLPARVPGLVARVALRGLQLIEARDDAWIVEAAAG
                     EAWHDVVSACVARGWDGLENLALIPGTAGAAPVQNIGAYGVELADRFDSLLAWNIPER
                     RLVRMTAADCRFAYRDSAFKHAQPGVWLIVSVRLALPRPWRPVLDYPDLQRDAALAAS
                     GEPTARAIFDAVCRIRRAKLPDPAVIGNAGSFFKNPLVQARVRDDLLARFPGLVSYAQ
                     PDGRYKLAAGWLIDQCGWKGKSLGPAGVHDRQALVLVNRGGATARDIMALAAEIQRDV
                     RQRYGVTLEPEPVVA"
     gene            complement(5914..6705)
                     /gene="dapB"
                     /locus_tag="VGC69_08025"
     CDS             complement(5914..6705)
                     /gene="dapB"
                     /locus_tag="VGC69_08025"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY0295261.1"
                     /translation="MRIAIAGVRGRMGRMLIEAVLNSPDAQLAVALDRAGSPALGQDA
                     GAFLGRDTGVLVTDDLDALANADCLIDFTRPEASLAHLQACARHGTKVVLGTTGFDAA
                     GRAAIDAAAKQVAIVFAPNMSVGVNATLKLLDMAARILNSGYDVEVFEAHHRNKVDAP
                     SGTALKMGETVAAAWGVKLPEVATWTRHGETGVREPGTIGFSVARGGDIVGDHTVYFC
                     GTGERIEITHRSSSRATYAQGSLRAALFLAGKQNGLYDMQAVLGL"
     gene            complement(6724..7167)
                     /locus_tag="VGC69_08030"
     CDS             complement(6724..7167)
                     /locus_tag="VGC69_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY0295262.1"
                     /translation="MTALALALALAGCGSESWGFPYRASVQQGNWVTQEQITQLKTGM
                     TRDQVRFVLGSPTLTDALHADRWDYPYFFKPGHGDSQERKFTVWFTSDALARWSGDEQ
                     PEKQPFQLDKNKAEAAVQRAIDAAKEVPRPSTDGEGAARMGSGSN"
     gene            7383..7802
                     /gene="fur"
                     /locus_tag="VGC69_08035"
     CDS             7383..7802
                     /gene="fur"
                     /locus_tag="VGC69_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY0295263.1"
                     /translation="MTDQSDLKTMGLKATFPRLKILDIFRKSDQRHLSAEDVYRALIA
                     DSVEIGLATVYRVLTQFEQAGILARSQFDTGKAVFELNDGDHHDHMICTNCGSVTEFT
                     DADIEKRQHKVAKDHGFALESHAMVLYGICSNCQKIR"
     gene            complement(7817..>8517)
                     /locus_tag="VGC69_08040"
     CDS             complement(7817..>8517)
                     /locus_tag="VGC69_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY0295264.1"
                     /translation="GDLPTLRDSLHARLADMQAAADTHALRQQAEAAQAQYAAAAGKL
                     SAARRKVAKDLGKQVSLAMQTLAMKGGAFEVALIDGTPSAQGSEIVEFRVAGHTGATP
                     RPLAKVASGGELSRISLALSVMASRAARVPTLIFDEVDSGVGGAVAEVVGKLLRELGE
                     RHQVLCVTHLPQVAACGNAHYRVSKTESRGQTRSGIEPLAPEARVEELARMLGGIKIT
                     DTTRNHAKEMLGMA"
BASE COUNT         1498 a         2669 c         2755 g         1595 t
ORIGIN      
        1 ctgaggccgt ccggattggt gctgctatcc atgtaatagg cgtcggagta ggcgtagccg
       61 gccaatacgg tccagtcggg cgttatgttt ccggtcgcct tcagctcgat ccccttgctg
      121 cgcgccttgc cggccgcgac gtaatcccct tcgttgggaa acctcgggtc tgacatggcc
      181 cggtttgtct cggtcagctg gaatagcgcc gcggaagtgt tgagcttgcc gtcaagatat
      241 tcacccttga cgccgagttc gtattgttcg ccttcgatag gcttgatcag gttgccggat
      301 tcgtcccatt gaaggggttt tcacccgcga cgggattgaa gctgtcatgc caccaggtta
      361 cccgggcgcc ggtcacgagc gtcagcggat cggccagtct gagttagggc ttccagctcc
      421 ttgatggtga ccttgctcat gtcgtccctt ccggtttttg ccccactgga tcggcaggct
      481 atggggggct cagcggatcg tcatggccga attttatcaa taactatttg tatttattgg
      541 gtattttata ttccgtagag atccgtggag tttcactgga attcacgttt taattcgatg
      601 agatacaagg tgtagcgagt ttgaaccccg ccgtttcgac ccgtagtatc aagggcttac
      661 agtcgcaagg cggtaagccc ttttcttttc aggcttggat agaattgagc gtttgggatt
      721 tgagcaagaa cttcgggcac ggcttgaaca ccacgatttt ttttgcatca tgaaatttgc
      781 gcgcggcgcg gccacctagc cgcgttacgg ggggagtccg ctttcggtat ggccgtttac
      841 cgcatttcat cgaggacgga ggttccaata ttcgcccaac ggatgcggac gcttttcgaa
      901 ggccgtgtct tgcggctcat cccgctcatc ctgctcgcgc tcgcgtacgg actggccaat
      961 gcccaggacg cgcctgccgc cgacgccgca tcggcgcgcg aggcggacat cgcgcgcatc
     1021 atcgacggca tcatcagctt cacgcaatgg ccggccgcat ccggcaaggt gcggctttgc
     1081 ctcacgacgc cggttatcca cgccgacgcg ctggaggccg agctgtccgg gtcgacgcag
     1141 ctggcggacg tgcaagacct ggcgccggac gatttccgcc tcgattccga ttgcgatgtg
     1201 gtctatatcg agcaggtcga cgacgcgaac cgggcgcatc tgttccaacg ccttgtcggc
     1261 cgtcccgtgt tgaccatcgc cggcgtggcg agcggctgcc tcatgggcag cctgttctgc
     1321 ctctcgggca ccggcgtgcc cgcgacgttc tcggccaatc tcgacacgat tgcccgaagc
     1381 ggccttcgca tcaatcccaa agtcctgttc ctggcgcgtc cgcaccaagc aaaatgacaa
     1441 accgtccggc atccaacaac agccccacac agcccagaac gctttctcag gtgctgctgc
     1501 gcgcacacgt gggatcggca ttagcggcca tgtgcttcgt gggcttggcg ctgatggcgg
     1561 cggggctgct gcagttgcgc agcaccgtca accagaacct cgagctcgtg gcgcgctcga
     1621 tggccttcgc ggccgaggcg gccgtcgttt ttcgcgatgg cccggccacc tggtcggcga
     1681 tcgacgagat cgcctcgatg gcaagcgtgg aaagcgtcgc cgttaccgac atgcaaggcc
     1741 agacgctcgc cgtctggcgc aagcagcgcg ccaatacctc gatcgcgcac tggttccaga
     1801 acctcgactt cctggagcag tccgtccgcc tgcccgtgtt gagccatggc aggcgggtcg
     1861 gtgcgatccg ggtgcagagc agttccgaca gcatatgggg gttcctcgtc acgggcttgc
     1921 tgtggatatt ggcttgcgtc atcgccagca tcgtgatgac ggcctatgtg acgcagcgca
     1981 cgctccattc gatcgtgcat ccgcttcgcc agttcaccga ggtgacccgc cgcgtccgca
     2041 acagccgttc gtttggcgag cgcgtgccgc gggccgacct cgtcgagctg aacgacctcg
     2101 ccaacgactt caacagcctg ttgagcgaac tggagcacag ggaggccggc gcccagggcg
     2161 agtatcgcgc gctggagcat caggccacgc atgacagcct caccgggctg gccaaccgga
     2221 cggcgttcga gatccggctc aggcactcga tactgcgcgc gcaggcgaaa gggcgctcgt
     2281 tcgcgctgct gttcatcgac ggcgacaatt tcaagctaat taacgacaat tttggccatg
     2341 ccgcgggcga tctcgtgctc agggaggtcg ggcggcggat aagcgagcag ctacgcgaca
     2401 acgacgacgt cgcccgtttc ggcggcgacg aattcgcggt cattctcgac ccggtcaagg
     2461 acaagggcag cgccgagcga gtggccgggg cgattgccgc ggcgatggcc gtgcccatcg
     2521 cgctggagtc ggggcagacc ctcgtcgcgt cggtgagcgt gggcatggcc ctctatccgc
     2581 aagacgcgac cgacgaactc ggcctgctgg aacacgccga cgcgacgatg tatgcccgca
     2641 aggcgagccg cggcgacgcc ggaacacaca ctcaacccgc tgtttaaccc atcaggacaa
     2701 gatgagatga tcgataccac cgcaaagccc ctctggattc gcaagctgtt gattgcctgc
     2761 atggttgccg ggacggtggg gatggcggga tgccagtccc tgaaacacgg cctgacggcg
     2821 gagcagatcg cgacgctcaa gcagcagggt tttgtcgaga cgagccaggg atggacgctc
     2881 gatcaatcgg ccacggtgtt cttcgccacc aacgacgcga cgctgtcgga cgtcggcgcg
     2941 cgcagcatcg acggcgtcgc caaggcgctg cggggcgtgg gcctggtgca tctcaaggtg
     3001 gtggggtaca cggactcgac cggcaccgat gcctacaacg acacgttgtc caagcgccgc
     3061 gccgaagccg tcgcgggcga attcgtgaac gacggctttc cgtccgacgg cctcgagacg
     3121 gtcggcatgg gcaagcgcta ccccgtcgcg gacaacagca cggcgcaagg ccgagcgcag
     3181 aaccgccatg tcgcgatcat cgtgatcgcc gactgatccg cgccccctca gggggctatt
     3241 tccgggccgc gcttgcggat gtgttccacg agattgcggc gttcccgcgc cgaggccagg
     3301 ccgtgctcgc gatcggagaa cggcacctcc cggcgccgcg cggtgacgaa cagcaatagg
     3361 ccatcggaat cgttgagggg gtagtccttg cctgtgatct tggcttgccg gacgacggca
     3421 tcggtcagtg ccatagcggt actcttcttg aggagaggag aaacggacaa cccttcggtt
     3481 caattagttc gaattgatcg atttgacgtc tgccttcgcg tgcggccacc ccatcggcgg
     3541 gtccccccga gccagcgagc aggctgatct gttcgcctac ctgtcaggat cgcgccaacc
     3601 ggtttgtatt gaagggactg cctaaagctg ggcatgtaga agccttttta tcataatgat
     3661 catattaatc aatatggtta gaggtgggcc atgatcactg aagtcagcgc cgtgaacttc
     3721 cggcagaatc tgggagaaat gatcaaccag gtgcagtacc gcaacgacag tatcgtcatc
     3781 aacaaggacg gcaaacccgt cgcggccttg gtcgatgccg acctgttcgc ccgcatccgg
     3841 cgtatgcagg agcgtttcga cgccttgagc cgtcgcatca gcgatggcta tgccggagtt
     3901 ccggaggaag aaggcctggc tgaaattgag gccgccgtgg cggcagagcg gaaagcacgc
     3961 tagtgcccgg actgcgcgtg gtgctcgaca ccaatgtgct ggtttcgggt gcggatttct
     4021 ggacgcggca tggagcctga tgtcaggttg acaagaatct gctgaatggc cggaacgcat
     4081 tgggtacagg caagtttggc ggattggagc aagcgtggaa cacgcatgca ttaggtcaga
     4141 ttgacgcgct gctgcacgcc acggcgctgc gagcgtcaat tatcggccag actcggtcag
     4201 tcgttcgcac cgatatttgg gcgctcagag tcatctcctg tcccagaaac ggccagcgtc
     4261 atgcgatgct gcttgcctga gcagtggtag cggaaatgcc agtcgtagcc ctcccggcca
     4321 gcgtcgccta cggaagtact tccttggcgg ggcaaattat ggcctcaaat acggccttaa
     4381 aaacgctggc tgtaggtgga ggtccctgga ttgtcgtgga cgctccagaa acagaaaaag
     4441 gagccgaagc tccttttctc tgtgttccat ggatttctat agccttccat ggacaaaaat
     4501 ccttggagcg ggagacgagt ctcgaactcg cgacctcaac cttggcaagg ttgcgctcta
     4561 ccaactgagc tactcccgca tgggtcttgc tgggtcttgc atgaatcttg ctggcttgct
     4621 ttcactacct tgctttcgct gcctcaccct acgcatcgcg tatcatgagc gaagaaaaag
     4681 actatagcaa ttatccggtc atgatgtcaa accagccagt tccaaatcca caagccctgg
     4741 accttttccc cgaggcttgc gatcttgcct cgcgcaacac gctgggcctg gcatccaggg
     4801 cagaggcttg cgtgctgctg gcgcatcgtt cgcaactcga cgccttgtcc ggcctggcgc
     4861 ggcaatatgc gtcgctgctg atcctgggcg gcggcagcaa cctggtgctg ccggcccggg
     4921 tgcccggcct ggtggcgcgc gtggccttgc gcggtttgca actgatcgag gcgcgcgacg
     4981 atgcctggat cgtcgaggcc gcggcgggcg aggcctggca tgacgtcgtg tcggcctgcg
     5041 tcgcccgggg gtgggacggc ctggaaaacc tggccctgat cccaggcacg gcgggggcgg
     5101 ctcccgtgca gaacatcggt gcctatggcg tggaactggc cgaccgtttc gacagcctgc
     5161 tggcctggaa tattcccgag cgccgcctgg tgcgcatgac ggcggcggat tgccgctttg
     5221 cctatcgcga cagcgccttc aagcacgcgc agcctggcgt atggctgatc gtctcggtac
     5281 gcctggcctt gccgcgtccc tggcggcccg tgctggacta tcccgacctg cagcgcgacg
     5341 ccgcgctcgc cgccagcggc gaacccacgg cccgcgccat cttcgacgcc gtctgccgca
     5401 tccggcgcgc caagctgccc gacccggccg tcatcggcaa tgcgggcagt tttttcaaga
     5461 acccgctggt ccaagcccgt gtgcgcgacg acctgctggc ccgctttccg ggactggtgt
     5521 cctacgcgca gcccgacggc cgctacaagc tggcggccgg ctggctgatc gatcaatgcg
     5581 gatggaaggg caaatcactg ggccctgccg gcgtgcatga tcgacaggct ctggtgctgg
     5641 tcaatcgcgg cggcgcgacc gcccgggaca tcatggcgct ggccgcagaa attcagcgcg
     5701 atgtccgcca gcgctatggc gtaacgctag aacccgagcc cgtcgttgcc taatgcgttt
     5761 tttgacgcta tggcttgcca ggtcaagagc cgaggacccc ggagcaatcc gaagcgcagg
     5821 cgctatcagc gccgagcatc ggaattgcgc agggcagcct cggcggctaa agaacccaag
     5881 ctgcagcggc ttaaagcaag cactacgctt actttacagc cccagcacgg cctgcatgtc
     5941 gtacaggcca ttctgcttgc ccgccaggaa cagcgcggca cgcagcgaac cctgcgcata
     6001 ggtggcgcgg ctgctcgaac gatgcgtgat ctcgatacgc tcacccgtgc cgcagaaata
     6061 caccgtatgg tcgcccacga tgtcgccgcc gcgcgccacc gagaaaccga tcgtgcccgg
     6121 ctcgcgcacg cccgtttcgc catgccgcgt ccaggtggcc acttcgggca atttcacgcc
     6181 ccatgccgcc gccacggtct cgcccatttt gagcgccgtg cccgaaggcg cgtcgacctt
     6241 gttgcggtga tgcgcttcga acacctcgac gtcatagccc gagttgagga tgcgggcggc
     6301 catgtccagc agcttgagcg tggcgttgac gcccacgctc atattgggcg cgaacacgat
     6361 ggcgacctgt ttggccgccg cgtcgatggc ggcgcggccc gccgcgtcga agcccgtggt
     6421 gcccagcacg accttggtgc cgtggcgcgc gcaggcttgc aggtgggcca gcgaggcttc
     6481 cgggcgggtg aaatcgatca ggcaatcggc gttggccagc gcatcgagat cgtcggtgac
     6541 gagcacgccc gtgtcgcggc ccaggaacgc gcccgcgtcc tggcccagcg cgggcgagcc
     6601 cgcgcgatcc agggccacgg ccagttgggc gtccggcgaa ttgaggacgg cttcgatcag
     6661 catgcggccc atgcggccgc gcacgccggc aatggcgata cgcataggaa ttccgggaaa
     6721 aagttagttg gagccggagc ccatccgggc ggcgccctcg ccgtcggtcg aggggcgggg
     6781 cacttccttg gcggcgtcta tcgcgcgctg gacggccgcc tcggccttgt tcttgtcgag
     6841 ctggaagggc tgcttctcgg gttgttcatc gccggaccag cgcgccaggg cgtcgctcgt
     6901 gaaccagacg gtgaacttgc gttcctgcga atcgccgtgg ccgggcttga agaaataggg
     6961 gtagtcccag cggtcggcgt gcagcgcgtc ggtcagcgtg gggctgccca gcacgaagcg
     7021 cacctggtcg cgggtcatgc ccgtcttgag ctgggtgatc tgctcttgcg tgacccagtt
     7081 gccctgctgg acactggcgc gataggggaa gccccaactt tccgagccgc aaccggccag
     7141 ggcgagggcc agggctaggg cggtcaggcc ggcccgggcg tagcgcagcg tgcggccagg
     7201 ggccgacggg tgggcggagg ggcaatgctg gcgaggatcc atgaacgcgg gtgacccggt
     7261 gaagtttaga aaaaggaaac aggtaaaacc gttatcataa aggtatcagc gagacagcag
     7321 cagccggggc catatggcct ggactgcttt atgaattaca tccagccgcg ggtgatcaaa
     7381 aaatgaccga ccagagcgat ctcaagacca tgggcctgaa ggccactttt ccgcgcctga
     7441 agatactgga tattttccgt aaatcggatc agcggcatct tagcgccgaa gacgtctatc
     7501 gagcgctcat cgccgatagc gtcgaaatcg gcctggccac cgtgtaccgg gtgctgacgc
     7561 agttcgagca ggccggcatc ctggcccgca gccagttcga taccggcaag gccgtgttcg
     7621 agctcaacga cggcgaccac cacgatcaca tgatctgcac caactgcggc tccgtgaccg
     7681 aattcaccga tgccgacatc gaaaagcgcc agcacaaggt agccaaagac cacggctttg
     7741 cgctggaaag ccacgccatg gtgctctacg gcatctgcag caactgccag aagatccgct
     7801 aggcataggc caggcatcac gccatgccga gcatttcctt ggcatggttg cgcgtggtgt
     7861 ccgtgatctt gatgccgccc agcatgcggg ccagttcttc cacgcgggcc tcgggcgcca
     7921 ggggctcgat gcccgagcgg gtctggccgc ggctttccgt cttgctgacc cgatagtgcg
     7981 cgttgccgca ggcggccacc tggggcaggt gcgtgacgca cagcacctgg tggcgttcgc
     8041 ccagttcgcg cagcagcttg cccacgactt cggccaccgc gccgcccacg ccgctgtcca
     8101 cctcgtcgaa aatcagggtg gggacgcgcg ccgcgcggct ggccatgacc gacagcgcca
     8161 gggaaatgcg cgacagttcg ccgcccgatg ccaccttggc cagcgggcgc ggcgtggcgc
     8221 cggtatggcc ggccacgcga aactccacga tttcgctgcc ctgggctgac ggcgttccgt
     8281 cgatcagggc cacctcgaag gcgccgccct tcatggccag ggtctgcatg gccaggctga
     8341 cttgcttgcc gaggtccttg gccaccttgc ggcgcgcggc cgagagcttg ccggccgcgg
     8401 cggcgtattg cgcctgggcg gcctcggctt gctggcgcag cgcgtgggtg tcggccgcgg
     8461 cctgcatgtc ggccagccgg gcatgcaggc tgtcgcgcag cgtgggcagg tcgccgg
//
LOCUS       DASWPO010000146         3939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2174730, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000146 DASWPO010000000
VERSION     DASWPO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174730"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1204
                     /locus_tag="VGC69_08045"
     CDS             311..1204
                     /locus_tag="VGC69_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0295265.1"
                     /translation="MTGLTSPAAQALRVEPAAAPLGARIHGVDLSVPLDDDTLARIEA
                     ALHRYGVVVFPEQRLTDAQQKRFSLRFGESLDIHSLRKFARPEHPEVFVLSNILVDGE
                     PIGAADAAQYWHTDLSYMQRPSRVSILYALEVPRDASGPVGDTEFVSTVQAYEDLPGS
                     VKAQLQGKRAMHLAGKPKKSQTSHFTKPLPTDIEDKLTGGVTHPVVRTHPFTGKKCLY
                     VNEGFTVRIEDMPGEQSDSLLATLFDHMIRPERIYTHKWTVGDVVMWDNCATVHQGVP
                     NYGPKQRRLMHRTIVTGSVPF"
     gene            1204..2208
                     /locus_tag="VGC69_08050"
     CDS             1204..2208
                     /locus_tag="VGC69_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295266.1"
                     /translation="MRLFPRSFFTGMAAALLAGLAALGLPAAQASAGTDDYPRSPIRL
                     VIPFVPGGAVDTVGRLLASSAGQILSENIIVENKGGAGGSIGASWVAKSAPDGYTLLL
                     GTSSTHGTNSATMPNLPYDPVHDFAPVAQVVEMPFILVANAAVPANNAAELIAYARAN
                     PGKVTFASWGPGSSNHLATELFMAAARITMTQVPYKGAGPAMVDVMSGQVDVTMDTFA
                     TSTPFIKSGKVKLLGVDSAKRSPDYPAVQTLEEAGAPGSEAGSWFGIFAPAGTPAPIV
                     GKLQAAFLAAVGKPEVQEKLQALGVMPSPADAGQLARRVTDEIGKWQAIVRSRGLKFD
                     "
     gene            2340..3923
                     /locus_tag="VGC69_08055"
     CDS             2340..3923
                     /locus_tag="VGC69_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295267.1"
                     /translation="MTVIASLGQTTLAASVPVPSSLPAASSLAQTGSVPSPTPSSSTT
                     VTLSQANPAPALVYLPPPAQPVWETQSADAATKLMAQNFSASTLAGRLQGLGQTLLEQ
                     LGGGGGDFSQSVYLPTGNAVPSSALKSVTQSILHTSADNQISLDVTTRSGATVQITLD
                     SQGNGLAAAVTVTGGTLTDADRSALLKLAGGFQDAIDGLTADPPALNLDGLVQFDPTV
                     LASVDLHASVDVSGQGAQTVDFHADDQQRSVKASGPLGTVQVNVDMSNPTLLGSADQQ
                     ARALGNYLQQFDAARSRGDGNAELMSMFSDAFKALNSHYAVNPAQPAASQSITLGDTD
                     RSMLTGLADFSASITQANQSLNPMKPGEVDGFAYQVSQHTAIQAGGRLNYTVNQTQQS
                     QLHASFHRTLQPGDTLSLTGDKQSQNYYYYQIDDSAQSQATIAYAKGKLVQATLNQSA
                     SESTRVQKYVMGDLEADTTTPYQQAWSQNLLDLLDSSQQSSPGMSSQANRQALLSRIN
                     DLVALPSSPGQLADKQPAR"
BASE COUNT          746 a         1415 c         1143 g          635 t
ORIGIN      
        1 tggcggatgc gccggcaggc ggcctgaggt tcgcgacggc agttcaaccg tacacacttg
       61 tttttcaggg tattgccagc aattcgaaat atccctcgac acagccaggc acggggtttc
      121 tccctaaata attccaatca atttgggcat agcatgtctt cgaatcgtgt gcggtccttt
      181 tcctgattct cgaaaaatcc agcgcataga ctcttttcag caatccggaa ccgagtcggc
      241 caagaccgct gcgacagcca cctgccatgt ctcgcgcgtc tgttcccgtc atcggccagg
      301 agttcttcga atgacaggac ttacctctcc cgccgcccag gcattgcgcg tcgagcccgc
      361 cgcggccccg ctgggtgcgc ggattcacgg cgtggatctg tccgttcccc tggacgacga
      421 caccctcgcc cgcatcgaag cggctttgca tcgctacggc gtggtcgtct ttccggaaca
      481 gcggctcacc gacgcgcagc aaaagcgctt cagcctcagg tttggcgaat cgctggatat
      541 ccactcgctg cgcaagttcg ccaggccgga acacccggaa gtattcgtgc tcagcaacat
      601 cctcgtcgat ggggagccca tcggcgcggc ggacgcggcc cagtactggc acaccgacct
      661 gtcctatatg cagcgcccaa gccgcgtctc catactctac gcactcgaag tcccgcgcga
      721 tgcaagcggg cctgtcggcg ataccgaatt cgtttccacc gtccaggcct atgaagacct
      781 gcccggctcg gtcaaggcgc agctgcaagg caagcgggcc atgcacctgg ccggcaagcc
      841 caagaaaagc cagacctcgc atttcaccaa gccgctgccg accgacatcg aggataaatt
      901 gaccggagga gtcacccatc cggtcgtgcg cacgcacccc ttcacgggca agaaatgcct
      961 ctacgtgaat gaaggattca cggtgcgcat cgaggacatg ccgggcgaac aaagcgattc
     1021 gctgctcgcc accctgttcg accacatgat ccgacccgag cgcatctata cgcataaatg
     1081 gacagtcggc gatgtcgtga tgtgggacaa ctgcgctacc gtccatcaag gcgttcccaa
     1141 ctatggtccc aagcagcgcc gcctcatgca ccgtaccatc gtgacgggat cggtaccttt
     1201 ctgatgcgcc tcttccctcg ctcctttttc acaggcatgg ccgcggccct gctggccggg
     1261 ctggcggccc ttggcttgcc ggcggcgcaa gccagcgccg gcacggacga ctacccccgc
     1321 tcgccgatcc ggctggtgat tccctttgtt ccaggcggcg cggtcgacac cgtgggacgc
     1381 cttctcgcca gcagcgccgg ccagatcctg agcgaaaaca tcattgtcga aaacaagggc
     1441 ggcgccggcg gctccatcgg cgcgtcatgg gtcgccaagt cggcgccgga cggctatacc
     1501 ctgctgcttg gcacgtccag cacgcacggc accaattcgg ccaccatgcc gaatcttccc
     1561 tatgaccccg tgcacgattt cgcgccggtg gcccaggtcg tcgagatgcc gttcatcctc
     1621 gtcgccaacg ctgccgtacc cgcgaacaac gcagccgagc tcatcgccta tgcgcgcgcc
     1681 aatcccggca aggtcacgtt cgcctcgtgg ggtcccggca gctccaatca cctggccacg
     1741 gaattgttca tggccgccgc caggatcacg atgacgcaag tgccctacaa gggagccggc
     1801 ccggcgatgg tcgacgtcat gagcgggcag gtcgatgtga ccatggacac cttcgccacc
     1861 tcgacgcctt tcatcaagag cggcaaggtc aagctcctcg gcgtcgacag cgccaagcgc
     1921 agcccggact accctgccgt ccagacgctc gaggaagcgg gcgcgccggg ttccgaggcc
     1981 ggcagctggt tcgggatttt cgcgccggcc ggcacgccag cccccatcgt cgggaaactc
     2041 caggcagcct tcctcgccgc cgtcggcaaa ccggaagtcc aggaaaaact gcaggccctg
     2101 ggcgtcatgc ccagcccggc cgatgccggc caactggccc ggcgcgtcac ggacgaaatc
     2161 ggcaaatggc aggccatcgt gcggtcgcgg ggactgaaat tcgattgagg cggccccgct
     2221 ggtaacaaaa gagtcaccct ccggcattta attcccggcg ggaactgccg taactggatc
     2281 agactcagca ttcccaaaat gggcggccag atcgcggtcg agcacgcccg ggcttcctca
     2341 tgactgtcat tgcttccctg ggccagacta cgctggccgc atccgtgccg gtcccgtcca
     2401 gtcttcccgc cgcaagctcc ctggcgcaga ccggcagcgt cccgtcccca accccgtcct
     2461 cctcgaccac ggtcacattg agccaggcca acccggcgcc ggccctggtc tacctcccgc
     2521 cgcctgcgca gcccgtctgg gaaacccagt cggccgatgc ggccaccaag ctcatggccc
     2581 agaatttctc cgcctcgacg cttgcgggac gcctgcaggg cctgggccag accctgctgg
     2641 agcagcttgg cggaggcggc ggcgatttct cgcaatcggt gtacttgccg accggcaatg
     2701 cggtgccaag ctcggcgctc aagagcgtta cgcagtccat cctgcatacc agcgccgaca
     2761 accagatctc gctggacgtc accacgcgca gcggcgccac ggtacagatc acgctcgaca
     2821 gccagggcaa cggcctggct gccgccgtca cggtcaccgg cggcacgctc accgacgcgg
     2881 accgctcggc cctcttgaag ctggccggtg gattccagga tgccatcgac ggcctgacgg
     2941 ccgacccgcc ggccctcaac ctcgacggac ttgtgcaatt cgaccccacg gtgctcgcct
     3001 cggtcgacct gcacgcatcc gtcgacgtca gcggacaggg cgcgcagacc gtggacttcc
     3061 acgccgacga ccagcagcgc tcggtcaagg caagcggccc gctgggcacg gtgcaggtca
     3121 acgtggacat gtccaacccc accctcctgg gcagcgccga ccagcaggcc agggcgctcg
     3181 gcaattatct gcagcaattc gacgccgcgc gcagccgcgg cgacggcaac gccgagctca
     3241 tgtccatgtt ctcggacgcc ttcaaggcac tgaacagcca ctacgccgtc aacccggccc
     3301 agcccgccgc gtcgcaatcg atcacgctcg gcgacaccga ccgcagcatg ctgaccggcc
     3361 tggccgactt cagcgcctcg atcacgcagg ccaaccaatc gctcaacccc atgaagccgg
     3421 gcgaggtcga cggcttcgcc taccaggtct cgcagcacac cgcgatacag gccggcggcc
     3481 ggctcaacta cacggtcaac cagacacagc agtcccagct gcatgccagc tttcaccgga
     3541 cgctgcagcc cggcgacacg ctctcgctga ccggcgacaa gcaatcgcag aactattact
     3601 actaccagat cgacgacagc gcgcagagcc aggcaacgat tgcctatgcc aagggcaagc
     3661 tggtgcaggc cacgctgaac cagtccgcca gcgagtccac gcgcgtgcag aaatacgtga
     3721 tgggcgatct cgaagcagac accaccacgc cttaccagca ggcgtggtcc cagaatctgc
     3781 tcgacctgct cgacagttcg cagcagtcca gtccgggcat gtcgagtcag gccaaccggc
     3841 aagcgctgct gtcgcgcatc aacgacctgg tcgccctgcc gtcgagcccg ggccagttgg
     3901 ccgacaagca gccggcccgc taggcttcgg accgggcag
//
LOCUS       DASWPO010000147         5844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2352848, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000147 DASWPO010000000
VERSION     DASWPO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352848"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VGC69_08060"
     CDS             complement(<1..1102)
                     /locus_tag="VGC69_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0295268.1"
                     /translation="MSKIIGIDLGTTNSCVAVVEGGQVKIIENAEGARTTPSIIAYMD
                     DGEILVGAPAKRQAVTNPKNTLFAVKRLIGRKFEEKAVQKDINLMPYSIVRADNGDAW
                     VQARDQKLAPPQVSAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKQDKADRKIAVYDLGGGTFDVSIIEIADVDGEKQFE
                     VLSTNGDTFLGGEDFDQRIIEYIISEFKKDQGVDLSKDVLALQRLKEAAEKAKIELSN
                     SAQTDINLPYITADANGPKHMNVKLTRAKLESLVDELIERTIAPCRTAIKDAGVSVSD
                     INDVILVGGMTRMPKVQEKVKEFFGKEPRKDVN"
     gene            complement(1341..1871)
                     /gene="grpE"
                     /locus_tag="VGC69_08065"
     CDS             complement(1341..1871)
                     /gene="grpE"
                     /locus_tag="VGC69_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0295269.1"
                     /translation="MTAPQGPIDPIPEPQPEADLQTELNTLRTELESVQAALQAQQEQ
                     VLRAMAEAENVRRRAQEDVSKARKFGIESFAESLVPVKDSLEAALAQADQTVDTLREG
                     VEMTLKQLANAFERNLLKEIAPAQGDKFDPHQHQAISSVPTEQPANTVVQVLQKGYAI
                     SERTLRPALVVVAAAK"
     gene            complement(1933..2970)
                     /gene="hemH"
                     /locus_tag="VGC69_08070"
     CDS             complement(1933..2970)
                     /gene="hemH"
                     /locus_tag="VGC69_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY0295270.1"
                     /translation="MSADAQLPQAAAGRTGVLLVNLGTPAAPTPRAIRAYLGEFLSDP
                     RVVELPRLLWLPILHGLVLRGRPGKLAPRYAGIWQEGGSPLLVYSRRQAEGLAQRLAD
                     RGLDVSVALAMRYGRPSVAEAMAALQAGGCEHILVVPLYPQYAASTTATAVDAVTALA
                     RRLRDQPALRFIKRFYDDPGYLDALAGRIAGFWQEHGRPDKLLMSFHGLPRQAVERGD
                     PYYRDCLQTAALLRERLGLTGDQAEVTFQSRFGAARWLEPYTEPRLRSLPGEGVASVD
                     VVCPGFVADCLETLEEIDQLGRAAFLQAGGRQFRYIPALNDSAAWLDGLAALVASNLH
                     GWPMGRAENPS"
     gene            complement(2976..3989)
                     /gene="hrcA"
                     /locus_tag="VGC69_08075"
     CDS             complement(2976..3989)
                     /gene="hrcA"
                     /locus_tag="VGC69_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY0295271.1"
                     /translation="MDDRALALLKALIERYIADGQPVGSRTLSKVFDLSPATIRNVMA
                     DLEDQGLIHSPHTSAGRIPTPRGYRMFVDSLLAVRSYQFEPGYMHDAAEMLPMSEPAR
                     AVNAAAALLSHLTQFAGVVLTPKRSQVFRQIEFIRLSDKRVLLIIVTPDGDVQNRILS
                     MQRDYTESDLLEAANFFNIHFSGKSFDAVRRTLSTELAQLREDISKLMQAAVEAGAEA
                     ADDGDTMVISGERKLLDVTDIASDMDRLRKMFSLFEKKTDLLQLLDMSSRAQGVQIYI
                     GGDSQLMPVEEVSIITAPYGVDGKVVGTLGVIGPTRMAYERVIPIVDITARLLSNALS
                     HNL"
     gene            4186..5100
                     /locus_tag="VGC69_08080"
     CDS             4186..5100
                     /locus_tag="VGC69_08080"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY0295272.1"
                     /translation="MHFPTIALIGRYQDSGLDAPLRALAGMLTQAGRTVLIEADTARN
                     TQVHEYQAASMEEIGKSASLAVVMGGDGTVLGAARHLAPYGVPLVGINHGRLGFITDI
                     ALQEAQTALAQLLHGNYRIEERMLLTGSVWRGNQQMYTATALNDVVINRAGRGGMIEV
                     HVELDDVYMYAQRADGLIITTPTGSSAYSLSAGGPLLHPGLNAVALVPVAPQTLSNRP
                     IVVPGDGVLKLTLTAMGRGEFGANVHFDMQSWSDLQAGDCIQVQRAPCTIRFVHPQGY
                     SYFSTLRRKLHWNLMPQEITEPGDGEAD"
     gene            5119..>5844
                     /locus_tag="VGC69_08085"
     CDS             5119..>5844
                     /locus_tag="VGC69_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0295273.1"
                     /translation="MLRTLHIRDFVIVEQAEIHFDAGFTVFSGETGAGKSILIDALAL
                     ALGDRGDAGVLRVGAERADITALFDTPDTLQGWLAERELDAGPELTLRRVIDAQGRSR
                     AYLNGNPVTVALLRELGEYLVDIHGQHAHQSLMRPDAQRDLLDAHGGHAALRQAVGQA
                     WKHWRARLKQLDAAEHDAGGLAAERERLQWQAEQLDALAPAPGEWDALQAEHTRLAHA
                     QSLRDGAAQVIEALDGENGSARQR"
BASE COUNT          901 a         1960 c         1965 g         1018 t
ORIGIN      
        1 ggttcacgtc cttgcgcggt tccttgccga agaattcctt caccttctcc tgcaccttgg
       61 gcatgcgggt catgccgccg accaggatca cgtcgttgat gtccgacacg ctcacgccgg
      121 catccttgat ggcggtgcgg cagggcgcga tcgtgcgctc gatcagctcg tcgaccaggc
      181 tttccagctt ggcacgcgtg agcttcacgt tcatgtgctt cgggccgttc gcatccgccg
      241 tgatgtaggg caggttgatg tcggtctggg cgctgttcga cagctcgatc ttggcctttt
      301 ccgcagcttc cttcaggcgt tgcagcgcga gcacgtcctt gctcaggtcg acgccctggt
      361 ctttcttgaa ctcggaaatg atgtattcga tgatgcgctg gtcgaagtct tcgccgccca
      421 ggaaggtgtc accgttggtc gacagcactt cgaactgctt ctcgccatcg acgtccgcga
      481 tctcgatgat cgacacgtcg aaggtgccgc caccgaggtc atacacggcg atcttgcggt
      541 cagccttgtc ctgcttgtcc aggccgaagg ccagggcagc cgcggtcggc tcgttgatga
      601 tgcgcttgac gtccaggccg gcgatgcggc cggcgtcctt ggtggcctgg cgctggctgt
      661 cgttgaagta ggccggcacg gtgatcacgg cctcggtgac ttcttcgccg aggtagtctt
      721 cggcggtctt cttcatcttg cgcagcactt cggccgacac ctgcgggggt gccagcttct
      781 ggtcgcgggc ctgcacccag gcgtcgccgt tgtccgcgcg gacgatgctg tagggcatca
      841 ggttgatgtc cttctgcacg gccttttctt cgaacttgcg gccgatcagc cgcttgacgg
      901 cgaacagggt gttcttgggg ttggtgaccg cctggcgctt ggccggcgcg cccaccagta
      961 tctcgccatc gtccatgtag gcgatgatgg agggcgtggt gcgggcgcct tcggcgttct
     1021 cgatgatttt cacctggccg ccttccacga ctgccacgca gctgttggtg gtacccaggt
     1081 cgatgccgat gatcttgctc atgagatgct taccttgaag tgttgattcg aaaaatgggt
     1141 gggaatgctt ggatgattga taaatggggg agtgtggctg cttttcaagg tcttttgggt
     1201 tcttaaagcg ccgaggctga cctgcgcaat tccgatgctc gcccccggtg ggggctgcgc
     1261 ttcggattgc tccggggtcc tcggctcgca attgccccta attacctgga aaacccatag
     1321 ctctccgcca tgacgattct ttacttggcc gcggccacga ccactagcgc cgggcgtagc
     1381 gtgcgctcgg agatggcata gcccttttgc aagacttgca ccacggtatt ggccggctgc
     1441 tcggtgggaa ccgacgagat ggcctggtgc tggtgcgggt cgaacttgtc gccctgggcc
     1501 ggcgcgattt ccttgagcag gttgcgctcg aaggcgttgg ccagctgctt gagggtcatt
     1561 tcgacgcctt cgcgcagggt gtcgaccgtc tggtcggcct gggccagggc ggcctcgagg
     1621 ctgtccttga ccggcaccag gctctcggcg aaggattcga tgccgaactt gcgggccttg
     1681 gatacgtctt cctgcgcgcg tcggcgcacg ttctcggcct cggccatggc gcgcagcacc
     1741 tgctcctgct gggcctgcag ggcggcctgg acggattcca gctcggtgcg cagggtgttc
     1801 agctcggttt gcagatcggc ctcgggctgg ggctcaggaa tggggtcgat cggcccttgg
     1861 ggtgccgtca tgggagtcct ccggaatgtt acagatacct attcttccaa gatggggatt
     1921 cccgccggga tttcaagagg gattttccgc cctgcccatc ggccagccat gcagattgct
     1981 ggccaccaat gccgccaggc cgtcgagcca ggccgcgctg tcgttgagcg cgggaatgta
     2041 acggaactgc ctgcccccgg cttgcaaaaa ggccgcccgg ccgagctggt cgatttcttc
     2101 cagggtctcg aggcagtcgg ccacgaagcc gggacagacg acatccaccg aggccacgcc
     2161 ctccccgggc aaggaccgca gccggggttc cgtatagggc tcgagccaac gggccgcccc
     2221 gaagcggctc tggaaggtca cctcggcctg gtcgccggtc agtcccagcc gctcgcgcag
     2281 cagggccgcc gtctgcaggc agtcgcggta gtacgggtcg ccccgctcga ccgcctggcg
     2341 cggcaagccg tgaaagctca tcaggagctt gtccggccgc ccgtgctcct gccagaagcc
     2401 ggcgatgcgg ccggccaggg catccaggta gcccggatcg tcgtagaagc gtttgatgaa
     2461 gcgcaaggcc ggctggtcgc gcaggcgcct ggccagcgcc gtcacggcgt cgaccgccgt
     2521 ggcggtggtg ctggccgcgt actgcgggta cagcggcacc accaggatgt gctcgcagcc
     2581 gccggcctgc agcgccgcca tggcctcggc caccgagggc cggccgtagc gcatggccag
     2641 cgccacgctc acgtccaggc cccggtccgc caggcgctgc gccaggccct cggcctggcg
     2701 gcggctgtag accagcagcg gcgagccgcc ctcctgccag atgccggcgt agcgcggcgc
     2761 cagcttcccg ggacggccgc gcaggaccag gccgtgcagg atgggcagcc acagcaggcg
     2821 cggcagctcg accacgcgcg gatcggacag gaattcgccc aggtaggcgc ggatggcgcg
     2881 cggcgtcggc gccgcgggcg tgcccaggtt gacgagcagc acgcccgtgc gtcccgccgc
     2941 ggcctgggga agttgcgcgt cggcggacac gtcggctaca ggttgtggct gagcgcgttc
     3001 gacagcaggc gggccgtgat gtcgacgatg ggaatgaccc gctcgtaggc catgcgggtg
     3061 ggcccgatca cgcccagggt gcccaccacc ttgccgtcga ccccgtaggg cgcggtgatg
     3121 atggagacct cttccacggg catgagctgc gaatcgccgc cgatgtagat ctgcacgccc
     3181 tgcgcgcggc tggacatgtc cagcaactgc agcaggtcgg tctttttctc gaacagcgag
     3241 aacatcttgc gcaggcggtc catgtccgag gcgatatcgg tcacgtcgag cagcttgcgt
     3301 tcgcccgaga tcaccatggt gtcgccgtcg tcggcggcct cggcgcccgc ctcgacggcg
     3361 gcctgcatga gcttggaaat gtcttcgcgc agctgggcca gctcggtcga cagggtgcgc
     3421 cgcaccgcgt cgaaggactt gcccgaaaaa tggatattga agaagttggc cgcctcgagc
     3481 aggtcggact cggtgtagtc gcgctgcatc gacaaaatgc ggttctgcac gtcgccgtcg
     3541 ggggtgacga tgatgagcag cacccgcttg tcggacaggc ggatgaattc gatctggcgg
     3601 aacacctgcg agcgcttggg cgtgagcacc accccggcga actgcgtgag atgggacaag
     3661 agcgctgcgg ccgcgttgac cgccctggcc ggctcggaca tgggcagcat ttcggcggcg
     3721 tcgtgcatgt agccgggctc gaactggtag gagcgcacgg ccagcaggga gtcgacgaac
     3781 atccggtagc cccgcggcgt ggggatgcgg ccggccgagg tatgcgggct gtggatgagc
     3841 ccctggtctt cgaggtcggc catgacgttg cggatggtgg ccggcgacag gtcgaacacc
     3901 ttggaaagcg tgcgcgagcc gaccggctgc ccgtcggcga tgtagcgttc gatcagtgct
     3961 ttcagtagtg ccagtgcgcg gtcatccata cccctatttt agggaatctt tgcggatcag
     4021 gggcgttttt gccgccggac cggtcccaag gcaaaacgcc ccccttcggg gggcagcccg
     4081 tgcacagcgc gcaatgtggg ggctttttat cctgccgggc ggccaatcgc gcccctataa
     4141 tcggctcacc cgccacaccg acgaatacta cgggtctgaa acacgatgca cttccccacc
     4201 attgccctga tcggcaggta ccaggatagc ggcctggatg cgccgctgcg cgcgctggcc
     4261 ggcatgctga cccaggcggg ccgcacagtg ctcatcgagg ccgacacggc ccgcaatacc
     4321 caggtccatg aataccaggc agccagcatg gaggagatcg gcaagtccgc ctcgctggcc
     4381 gtggtcatgg gcggcgacgg caccgtgctg ggggccgccc ggcacctggc cccctacggc
     4441 gtgccgctgg tgggcatcaa ccacggccgc ctgggcttca tcaccgacat cgccctgcag
     4501 gaggcgcaga ccgcgctggc ccagctgctg cacggcaact accgcatcga agagcgcatg
     4561 ctgctgaccg gcagcgtctg gcgcggcaac cagcagatgt acaccgccac ggcgttaaac
     4621 gacgtggtca tcaaccgcgc cggccgcggc ggcatgatcg aggtgcacgt cgagctcgac
     4681 gacgtctaca tgtacgccca gcgcgccgac ggcctcatca tcaccacgcc caccgggtcg
     4741 agcgcctatt cgctgtcggc cggcggcccg ctgctgcacc ccggcctgaa tgccgtggcc
     4801 ctggtgccgg tggccccgca aaccctgtcc aaccgcccca tcgtcgtgcc cggcgacggc
     4861 gtgctcaagc tcacgctcac cgccatgggc cgcggcgagt tcggcgccaa cgtgcacttc
     4921 gacatgcagt cgtggtccga cctgcaggcg ggcgactgca tccaggtgca gcgcgcgccc
     4981 tgcaccatcc gcttcgtgca cccccagggc tacagctact tctccaccct gcgccgcaaa
     5041 ctgcactgga acctcatgcc gcaagaaatc accgagcccg gtgacggcga ggccgactaa
     5101 gcaatttgcc tcgtccccat gctgcgcacc ctgcacatcc gtgatttcgt catcgtcgaa
     5161 caggccgaga tccacttcga cgcgggcttc accgtgttct ccggcgagac cggcgcgggc
     5221 aagtccatcc tgatcgacgc cctggccctg gccctgggcg accgcggcga cgccggcgtg
     5281 ctgcgcgtgg gcgccgagcg cgccgacatc accgccttgt tcgacacccc cgacacgctg
     5341 caaggctggc tggccgaacg cgagctcgac gccgggccgg agctgaccct gcgccgggtc
     5401 atcgacgccc aaggccgcag ccgcgcctac ctgaacggca atccggtcac cgtggcgctg
     5461 ctgcgcgaac tgggcgaata cctggtggac atccacggcc agcacgccca tcaaagcctg
     5521 atgcggcccg acgcccagcg cgacctgctc gacgcccacg gcggacacgc cgcgctgcgc
     5581 caggccgtgg gccaggcctg gaagcactgg cgcgcccggc tcaagcagct cgacgccgcc
     5641 gaacacgatg ccggcggcct ggccgcggag cgcgaacgcc tgcaatggca ggccgagcag
     5701 ctcgacgccc tggccccggc gcccggcgaa tgggacgccc tgcaggccga acacacgcgc
     5761 ctggcccatg cccagtcgct gcgcgacggc gccgcccagg tgatcgaggc gctcgacggc
     5821 gaaaacggct cggcccggca gcgc
//
LOCUS       DASWPO010000148        38991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1729886, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000148 DASWPO010000000
VERSION     DASWPO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 38991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38991
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729886"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1422)
                     /locus_tag="VGC69_08090"
     CDS             complement(73..1422)
                     /locus_tag="VGC69_08090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0295274.1"
                     /translation="MSNLGQALRQPLERIRPDETLPVEVDVVVVGGGIAGCSTAWELA
                     RAGVKVALLEKGLVGAEQSSRNWGWCRQQGRDPKELELARLSLQIWPELSAQLERDVG
                     FRACGVTFLTESEAELGAWRTWKEMAAGYGVKSELLSAEQARRRMPGGTTGAWVGGLT
                     TAEDGRAEPGRVAPYMAMAAQKLGAVVAEQCAVDGVETANGRVAAVVTERGRIACASV
                     VVAGGAWTRLFLRKLGVAFPQAYVYGSVSRVQSDGTRMEDCIATPYFAVRERTDGGFT
                     VAKSGRGTVYLTPALLRQGWRFWPTYQVRRKNVSLSLARFWEEWRMERDYLVRGISPF
                     SSHRILDPEPDMDLLNDAVRRAGEMFPALSRSTIQSAWGGCIDSTPDAVPVMSGIQAL
                     PGLYVASGFSGHGFGLAPGVGRAMASLVQGKPAPIDMTPFSHARMLDGRRLAPYLLL"
     gene            complement(1439..3421)
                     /locus_tag="VGC69_08095"
     CDS             complement(1439..3421)
                     /locus_tag="VGC69_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0295275.1"
                     /translation="MNTSELQVFANFCVAAAESMGYALIRTAHSTFIKETEDFSCTIM
                     SVNGQTFASPRTLGATWYSGLDYEPLIRSVEQYEPGDIYWTNDAYSGSVATHTPDIVM
                     WKPVFHGDRIICFVGGHVHNTDMGGAVPASLSRTLTEVHQEGIRFPPMRIMSRAGGVN
                     EEAVRIMACNVRLPEQNIGDMRAQMASLTVGERKILEIVEKLGVEEFMDGVEKLMDYA
                     EMQAKEIVAAIPDGDYFFDDYADEDSAVDGRPMRVALNLQVRGSELLFDFTGSDPQLN
                     SSLNVPTGGRERHVLPLVGLAYVLYSLNPNMMLNYGMLRVARCILPKGSVVNAVHPAA
                     VGMRSLTCLVMQSVTFGAFSLALPQKMSASPSSGMSVMNVRTSDDAGNTIMASLGPVG
                     GGAGGNAREDGVEGSGANMAFLRNTPVEIIEAEIPIRMREYHLYPGSGGPGKFRGGLA
                     LVMEFQVFAPNTMVTARNRDKSFFGAWGVLGGKAGAASFFLLNPGTDREVSLANTDIV
                     HCEPNDVLRITGCAGGGYGNPMERDPQAVLRDCIRGFVSLGQARDDYGVVIRDGKVDE
                     AETRALRAQAPVPDPGRHFLHTPARTEHEKVWTRERYIALTDYLFRCPVTWRYFLKHH
                     LFREMEALPDELRHNGRTEVVRDLIAAANVKYHLNP"
     gene            complement(3435..5483)
                     /locus_tag="VGC69_08100"
     CDS             complement(3435..5483)
                     /locus_tag="VGC69_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0295276.1"
                     /translation="MSYRVGVDIGGSFADFAVLDTETNELVALKVFSRADAPGDDVKA
                     GLAKLREDHGIDLSRIEYFTHGTTVGVNAVVQGKGSRLGLIANRHFEDVFELARLKTP
                     DMYDLFSKRPAPIVPRERVRGIDGRMGPDGAEIDPLSVEQALEAARDLFEREQCEGIL
                     ISFLHAYRNPAHERSAKAAIMAQYPDRFVLCSSEVWPIVREFERTITAGVAAYTQPII
                     NRYLTRLQGVLDGLGVPADLHVTKSNGGVMRADTAKDDSIQVILSGTASGVIGASYIA
                     ERSGQPNCLSLDIGGTTADVAIIENATPHFTTGEYVGEYQIFIPSVSVNSIGDGGGSI
                     AWVDSFGVLKVGPESAGSNPGPVCFRRGGTRPTITDAYAVLGYIGHHPLSYGAVTLDR
                     EGAARAIGELAGKLGKTVHETAAAIIDVSVSGMYAGVSQLISRYGIDPKDYTLLPFGG
                     AGPMMACHFARAIRMKRILVPHTPGVLSALGGLIADTRNDFVKTTFYKLDEDYAPRLK
                     EDFLALRTTAADWIESETGQASNYGLEAIADMRYKGQSFEVPTALSVDDVTSLNIAGM
                     RASFNEQYQRIYGYSDTDAPIQIITLRVVVSAENPKPVMPRIAQADAEAQPAYRTELF
                     VDGSLCDTPVYGRETLRAGHRIAGPAIVTQSDTTISILPGFEAVVDPHGNILIEYQQH
                     "
     gene            complement(5553..6386)
                     /locus_tag="VGC69_08105"
     CDS             complement(5553..6386)
                     /locus_tag="VGC69_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0295277.1"
                     /translation="MTNAQQVGYGRFAMPRIGLGTWELDGERGEAAIDAALAAGYRHI
                     DTARRYGNEALIGRVLKARRVEREQLFITSKVWWTDLAYDDALRSIGESVARLDCAYL
                     DLALIHWPNPAVPLKDTLAAFARAKAQGLVRHIGVSNFPTALLREALAVSGEPIVANQ
                     CEYHPFLDQSAVLDFCRGHDMVFVAYAPLGSGALLRDTRIAGLAQRHGRQPSEIVLQW
                     LAQQPGVAAIPRSSDPGRLALNLGTGDFTLDDEDMRALNALRRPDGRLFDPAWSPAWD
                     V"
     gene            6452..7105
                     /locus_tag="VGC69_08110"
     CDS             6452..7105
                     /locus_tag="VGC69_08110"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0295278.1"
                     /translation="MKTVHPAAGAAKPDSGSAESDGLQQGPVAFGAKLRAHRKSRRIT
                     LLQLADWADLSPGLISQIERGVTSPSLRTLSKLRNALELPTSFFFEEEPGHAGYLQDP
                     SYICRAGDRPRLDLGLGAPHKELLHHGGSRVFEMMIVEIPPAGDSGPKVISYPSEKGG
                     MILQGQLELTVDGRRNLLDPGDSFLFDGSLPHQLRNPLDAPTRLLWIIAKLPNLHPF"
     gene            complement(7114..7788)
                     /locus_tag="VGC69_08115"
     CDS             complement(7114..7788)
                     /locus_tag="VGC69_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0295279.1"
                     /translation="MTNNGMREGEAAWDLDAPPSAGADDGPAGPPAHAAAFGQKLRAV
                     RKAKHMTLQELARRAEISPGLLSQVERGVSSPLLKTLLKLRNALDLPSSFFFEEDPPL
                     PQAEADPSYLCRAGDRPRLDMGDMAPHKELLHHNDSRVFEMMIIHIPPRTKVGPAAMQ
                     YPSEKGGLVLEGRVDLNVDGRSSLLSSGDSFLFDGSAPHFLYNPTDSMARTIWVVAKL
                     PNDSPF"
     gene            complement(7811..8830)
                     /locus_tag="VGC69_08120"
     CDS             complement(7811..8830)
                     /locus_tag="VGC69_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0295280.1"
                     /translation="MKFPRYAAFALALSAGQAAHAADLTVTAFGGSWEQAYRKCFVQP
                     FEKRTGKTVDVILGSPTQWINQISANPSKPPIDVMVGAVDSGMIARQRGLVDELTEKN
                     VPNITQINPTLLNYGAGYGFPITFGDLGLMYNTDTVKAPPKTWKEFVDGTIAGKWHAA
                     IPGIAYVATPQGLIAMLAKVYGGDLDNVQPALDQIKRMRDSGNVTFYNDPNTPLASLR
                     QGDIDIAMYFDGRAWAEHDAGAPMIGYLNPEPGAVAFPNLVQKVKNGSPLGWDFVNDL
                     AGAEGQACFANAMLYAASNKNVKYDPKVEPRVAVAEKSVWFPSDKIVANTPKWIEMWN
                     KQIGR"
     gene            complement(8925..9743)
                     /locus_tag="VGC69_08125"
     CDS             complement(8925..9743)
                     /locus_tag="VGC69_08125"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295281.1"
                     /translation="MSPALWSWMARRLPGMALGTYVILFVAFILAPIVIVVLASFTPH
                     LAVAVSFDGVSLRWYRRIWEYRPFADSLVTSLVLALASTFCAAVLAVPAALGLARSDG
                     RLAGMASSFLLSPLAVPPLVIGLSSLYFLSHIGLGVSFTALLITHTVTSVPYVMRTVV
                     ASCKGLSGSYAEAASVLGANPWKTFIHVTFPLILPGIFAGALFSFLISMDNLGLSLFF
                     GSARISTLPVVMLSYLENQFDPSIAAISTVQMLIAVVLLVVVEKIYGLRALTEH"
     gene            complement(9740..10621)
                     /locus_tag="VGC69_08130"
     CDS             complement(9740..10621)
                     /locus_tag="VGC69_08130"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295282.1"
                     /translation="MSASATTAAAPARAGWRRSQAFWLYLPALVILLVFFGLPLYESA
                     LQSLGISSIPGTPSAFTLQYYRKLFLDPYYLGLIGETLKVSAITTVLCLLAGYPIAYF
                     IVRRAGRWVGPLLFVLIMPLLTSIIMRTFGWNVLFARRGIVNVALMGMGLINQPLRVL
                     DGASIVYAGMVHVMVSFMVLSIIPVLRSVDRRLEESARVLGAGAMRTFFLVTLPLSME
                     GIITGCLIVFMITNGSFVTLLLLGNSSVVTLPLLIYQQFNQTQDMNFASAMGTLLLVI
                     VLACLYVQSRIGKGRAA"
     gene            complement(10618..11805)
                     /locus_tag="VGC69_08135"
     CDS             complement(10618..11805)
                     /locus_tag="VGC69_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295283.1"
                     /translation="MQKAADSGWLTAPRSSVGEAGHALPAKPLALAGISKRYGGVIAL
                     QRMSLDIRPGELVSLLGPSGCGKTTTLRLIAGFEFPDSGSIRIGGADIAHLPPNKRGL
                     GMVFQNYSLFPHLNVAENIAFGLRMAGASKAQQAEQVSRMLQMIRLDDYGARKVSQLS
                     GGQQQRVALARALVTNPSVLLLDEPLGALDKNLREGMQFELRALQRKLGITSVMVTHD
                     QEEALTMSDSVVVMNAGRILQVGPPREIYDYPRTRFVAEFLGAANILGYRLVNEEPQA
                     YVLAVLDAQDQPRFELRIPKTERPVRIRRKGELALRPEKIGLADASGAGLGFPGRIVN
                     HVFRGAQHAYQIDVPMLGSMISVYRHAGETEVREAGAQVHCLFKLRDVVVLDAETDPE
                     DEA"
     gene            complement(12014..12925)
                     /locus_tag="VGC69_08140"
     CDS             complement(12014..12925)
                     /locus_tag="VGC69_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY0295284.1"
                     /translation="MSQTFDLGALFTPLLKAVDPDLELYAWPDPRCLEAEVAVCWHTP
                     PGAYEKMPRLRLIHSIAAGVDNILGDASLHHIPVCRVVDPQQSEGMLQFILWSVITFH
                     RGLDGVLADQARHGWTRPEQPPASDFHVGIMGLGRLGQDVAQGLARFGYRVSGWARTA
                     RELAGVQVHAGPDRFETFLSGLDALVCLIPLTEQTRGILDRRTFSAMPRGAALIHCGR
                     GQHLVEADLVEALESGQLRGAVVDVFEREPLAPGHPLWNAPGLIVTPHMASVASFKCT
                     AEQVCANIARLNAGEPLMNRVDPAAGY"
     gene            complement(12993..13892)
                     /locus_tag="VGC69_08145"
     CDS             complement(12993..13892)
                     /locus_tag="VGC69_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295285.1"
                     /translation="MLSNVSDLDLRLIRVFLAVVDAYGVSAAQAKLNVGQSTISTQLA
                     TLETRLGYKLCERGRAGFRLTAKGEKFADAARRMLDALTEFGIEARNLDKKLVGSLSI
                     GLMGHTPSHENARLSDAIRRFRDRDEAVRLDLVVRTPGELEDMLLNGAVQVAVGYFWR
                     RVPALQYTPLMLERQVAYCAASHPLFAQAGSVSPEDAMTHQWAWRGYPVPAGMTPVPR
                     RQVTAVTGNMEAMSILILSGCHLGYLPQEIGQSYVDRGLMAALNPLELSYEVTIHVVT
                     SRAQAGDDLLNAFMEDLKSAQLL"
     gene            14026..15444
                     /gene="argH"
                     /locus_tag="VGC69_08150"
     CDS             14026..15444
                     /gene="argH"
                     /locus_tag="VGC69_08150"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY0295286.1"
                     /translation="MSEKLLWGARFRAAPDAALMNLSRSDASHFRLAAYDIMASQAHA
                     RELERAGLLTAAEREAIVATLGSLKERIARGELAPVAGDEDVHTFMERLLVAELGDTG
                     AKLRAGRSRNDQAANNLKMYLRDEARALVAELLALQDALVGQAGKHAQTLAPGFTHLQ
                     AAQPIVFGHQLLAHAQTFNRDASRFIDWDRRSSRSPLGGAAMAGSAIALHPELSAADL
                     GYEAPCENSIDAIGSRDHVAEFLFVSAMLGVHLSRLSEEITLWASRQFQWVKLDDAFS
                     TGSSIMPQKKNPDVAELTRGRAARLIGMLTTMLAALKGLPFSYNRDLAEDKYTAFEAV
                     DVLHKVLPAMAGLIRTLNVDVERLRRQAVEGYTLATEVADWLSRRGVPFATAHEITGA
                     LVKRCEDLGIELSEISDADLVAVDARLDPAIRAVLTPEAAVAARSGYGGTAPARVAEQ
                     IERLKAVLDKQAEWARAGVARC"
     gene            15438..16319
                     /locus_tag="VGC69_08155"
     CDS             15438..16319
                     /locus_tag="VGC69_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0295287.1"
                     /translation="MLKTGAGDPARGPATPADGISHYQHVPRRYWGRYLSAALIVLAI
                     LFVLNAFAHGQIEWSYVGRFFTAPTILRGLGNTIVMSIAAMALGVALGIITAVMRLSP
                     NPVLSTVARGYVWLFRGSPVILQLLLWFNLALVFPTLGIPGLFEAKTVDVMTPFLAAL
                     LGLGINQGAYTSEVVRAGLLSVDVGQYEAAKAIGMPRLQALRRIILPQAMRVIIPPIG
                     NEFVGMVKLTSLASVIQYAEVLHNAQNIYYANTRVIELLIVAGIWYVAVVTVLSLLQG
                     RTEAFFSRGSGRKSARR"
     gene            16363..17127
                     /locus_tag="VGC69_08160"
     CDS             16363..17127
                     /locus_tag="VGC69_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0295288.1"
                     /translation="MSTIVKAVDIRKAYGDFVALHGITLEVERGEVLCIIGPSGSGKS
                     TFLRCINQLEEISGGALWVDEELVGYRISGTKLHPLSEKEVARQRLKTGMVFQRFNLF
                     PHMTVLENVIEGPARVLRQPRAQALEQAQALLERVGLAAKKDAYPIELSGGQQQRVAI
                     ARALAMQPQLMLFDEPTSALDPELVGEVLAVMRDVARTGMTMIVVTHELSFAREVADR
                     IVFMDQGRVVESGDPRSLLSNPQHARTREFISAVLS"
     gene            17157..17990
                     /locus_tag="VGC69_08165"
     CDS             17157..17990
                     /locus_tag="VGC69_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295289.1"
                     /translation="MKTSSGKSLFTRAALACALSTAALMSAQAQGVPKELAAAIVPNY
                     PPLDYKDPATDELTGFDVDLGKALAAKAGTQLRWEETSFDQMMSAVATGRVNIILSGM
                     TDLASRHDAVHFVDYLKTGPQFYVMKANQAQYPDMQAMCGKKVGSSRRTSFPKEIAQW
                     SDDNCVKAGKPAIVVVGTDGSADAQTQLRQGRLDAAVQGGETLPYLQQQAPGTYTVVG
                     KPIAFQYVGLAVGLKDEALFKALSDAMAAIIADGTYAKITAKWHLEDFAVSKLITDSA
                     E"
     gene            18011..18871
                     /locus_tag="VGC69_08170"
     CDS             18011..18871
                     /locus_tag="VGC69_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009917210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY0295290.1"
                     /translation="MQSIDPGRPTWPDGRQSCVALAFDLDGPTGGAMLDGSLWHNPSL
                     FMLGAYEPWRALGYLLGLLRELRTPATFFVPAWVLDTWPEQCRAIVEDGHEVAYHGDQ
                     HEAFWQLDAGQQREVMARSKAVFARHLGLAPTGFRTPSGDWGPDTVDILLEAGVRYSS
                     SMRGDDRPYFIPGTDGARGLVEIPGRWEMDDYTSLAYQRRPDYPAGLDRIAGYDNTLD
                     NWRREFDGAHAEGLCLTTLLHPKVSCKPGRLGILERLIGHMRDQGNVWFATCAQVECW
                     WRDRTAKETP"
     gene            18868..19764
                     /locus_tag="VGC69_08175"
     CDS             18868..19764
                     /locus_tag="VGC69_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY0295291.1"
                     /translation="MNGLWPENRRSAATLTVDFFDDAFILAQEPRMAGRDKSLSVWRY
                     GAQRGVERLLRLFDDMAVRTTWFVPGCVAERHAALVRDIHAAGHEIGNATYACENFDA
                     LPLEAQADSARRAQDAIGQAIGQAPEAFRSPWGNWAPGLPVALLRAGLRWSSSWRGDD
                     RPYFHAGEPGAQDRLLEIPLHYELEDEPYFIFNLYPPVPSGQPRIAPYRDALSNLMQD
                     FAGFHKLGLCYPLRIHPEILGTAGRIGLLRELLEHISATPGAWLASGGEIAAWWRSRH
                     TSNEPGHPADLFAPRHRPAPTP"
     gene            19761..21152
                     /locus_tag="VGC69_08180"
     CDS             19761..21152
                     /locus_tag="VGC69_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0295292.1"
                     /translation="MTAPTYARQLARFATATAFADLPAPLVERAKRHILDTLGAALAG
                     SASTEARTVQAVLRQDAAGSGPARIWGLDRAIGQRDAAFANGVAAHALELDDAGGCDH
                     SGAVVLPAVLAAAAGAPEPVSGQDFIAAVVIGYDVGRRVLEACGGYPAHNGVGWHSTA
                     TCGTFGAAAAVARLWRLDEQQCAWALGHAASFSGGLWAFIHDGTQTKKLHTGRAAEGG
                     LLAARMATAGISGPAAVFEDVWGGFFRAFAPQTAQPEALTAELGETWKLLRCSIKPYA
                     ACRSTHASVDALLHLLANAEAGRQAAAVDVRLSQFVMNMCGGRDLTTLASAQMSMPYA
                     LAAAWVFGDANIERYLQGMRGDPRLAQAMARIRLHPDPAMADLEEPTITLSGDGMAPV
                     SHHVPIALGAPANPLSDAQLLAKFTQLACVALPPSQAEQLADRVLRLEADPDSRTLQD
                     LLLGHADAARLIA"
     gene            21251..22015
                     /locus_tag="VGC69_08185"
     CDS             21251..22015
                     /locus_tag="VGC69_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0295293.1"
                     /translation="MDNPLNVIDSEWTTRCELAALYRLVAHFRMTDMIDTHISARVPG
                     HQDQFLINRYGVLFHEMRASDLVKIDHQGKVIETGRSAEETRVNAAGFTIHSAVHMAR
                     HDLNFVIHTHTAAGIAVSAQEHGLLPISQHALRFYGKLSYHDYQGIALDLEERESLVA
                     DLGPTNKAMILRNHGLLGAGRTASGAFQEIYFLERSCQAQVAALSGSQQLRYPSETVC
                     QRTAGQYKGEETGFYGDIFWSGCLRLIENDQPDYRS"
     gene            complement(22036..23442)
                     /locus_tag="VGC69_08190"
     CDS             complement(22036..23442)
                     /locus_tag="VGC69_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295294.1"
                     /translation="MNGEAAQAWLAQGRAHLAGGRHMPAVEALQQASHAQPLNPEIYR
                     WLAQAMEAMGQAADALSARLACEALERKSAEDLYGIGRSYARHNQWAAAGYWFQRALL
                     LDPDMVYAYLYMGMALRETGHLAESESYSHNACLRQSLFIDEALPTQRRSVLVLCTSR
                     NDNVPVQFLLPKQRNRQIRWAIEYGLVGPVRELPPHDLVFNVVGEADSAQASHDAVAN
                     YIADIDKPVLNLPARITLTSRDRMPGLLADIDGVCMPPAVRHRPAQGLGLPQAIERAG
                     LAYPVTVRPVGRHGGQGLERLDTPQDAAAHPRADGDLYLSQYCDYRSPDGCFRKYRVV
                     LVDGEPWPYHLAVGDRWVLHYVTADMTAAPWKTEEERRFLDDPEAVLGTRAWQALREI
                     GRRLGLDYCGVDFAVLPDGRLLVFEANATMLVHPEEEGDAALRFKNPYVQRIVDAFDR
                     MLERRQAQARGRPLAPAA"
     gene            complement(23439..23699)
                     /locus_tag="VGC69_08195"
     CDS             complement(23439..23699)
                     /locus_tag="VGC69_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY0295295.1"
                     /translation="MNRTQWMEDLQKNISELIAKSPAADIERNIRSMMTQAFSRLDLV
                     TREEFDVQADMLTRARSKVDQLAEQLQQLQARLEAIEAGKGQ"
     gene            24176..24514
                     /locus_tag="VGC69_08200"
     CDS             24176..24514
                     /locus_tag="VGC69_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY0295296.1"
                     /translation="MKLIIAIIKPFKLDEVRVALSGIGVQGLTVTEVKGFGRQKGHTE
                     LYRGAEYAVDFLPKLRVEAAVPDHLVDQVVEAIEQAARTGKIGDGKIFTAPLEQVIRI
                     RTGESGEAAL"
     gene            24556..25806
                     /gene="amt"
                     /locus_tag="VGC69_08205"
     CDS             24556..25806
                     /gene="amt"
                     /locus_tag="VGC69_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY0295297.1"
                     /translation="MDKADISWMLVSTLLVLMMAVPGLALFYGGLVRSKNMLSVLMQV
                     TGAFTVGLILWFIYGYSLAFTDGNSFFGGFSRMFFSGMFNPADGKYAMNGSITEILYA
                     SFQATFAGITCALIVGSFAERARFSAVLVFTVIWFTFAYVPIAHMVWFAIPSGQQGTS
                     GLLAAAGALDFAGGTVVHINAGIAGLVGAYVIGKRVGYGRESMQPHNLVMTFIGAMLL
                     WVGWFGFNAGSAVAANELATMAFFNTMIATAAASLAWLFTEWAAKGKPSMLGAASGAI
                     AGLVGITPAAGFVGPVGAFVIGIVVGIICVWGVNGLKRLLRADDALDVFGVHGVGGIL
                     GALLTGVFSAKGLGGLSLDTMDAIGGQVLTQLEAIVITLVWSGVVAWIAYKIADMLCG
                     LRVPEDQEREGLDVTTHGESAYHS"
     gene            complement(25951..27357)
                     /locus_tag="VGC69_08210"
     CDS             complement(25951..27357)
                     /locus_tag="VGC69_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295298.1"
                     /translation="MMTVETGNQLGRSAGSTQESPQSNGVVNVSWLIDNAAFTWRHGL
                     VVMLCALVSLLDGADSQVIGIAARSIMGSLDIHAQAFGIVFSITHLGAALGALTIGSL
                     GDRWGRKSTLLIALLILGVFSIATAWADSLTSLTVLRFLAGIGIGGATPCFLSMVSDY
                     APLRRRGTLVAVAWSFFPLGGMLGGLLNSAMLARWGWQSVFLVGGGIPICIALVLFVL
                     VPETVQHLAAKGAAPSLIRARLKWLGISVPPSVERFVCDERKRRHGSIREVFGDHRKA
                     STFVLWAILAVSFATLSLVVTWTPILIHDKGIGDRAVPAILAGFNAGCFVGMGAAGYL
                     VDRFGPLRTLFVPFILAACAVASLPAAGSVAGAAAAATVLGLTCGMGCAGAIAVMTLL
                     YPIDIRATGIGFGMASTRTGQVVAPLVISLLISSGFGSEHLFFAMAMFPASAALFVVV
                     FCLIDRKRVIHANASALA"
     gene            complement(27476..28483)
                     /locus_tag="VGC69_08215"
     CDS             complement(27476..28483)
                     /locus_tag="VGC69_08215"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0295299.1"
                     /translation="MKVCVYGLGAVGGLIAGKLRSAGVDVCAVARGRVLEHVNRAGLA
                     LTSAGHTEHASLRVAENPAALGAQDLVVIAVKATGIGDVARRIAPLLGEHTVVLSAMN
                     GVPWWFYHGIREVGPGRRIETVDPAGAISAAIAPERVVGCVSNLSASTPAPGEVAYLP
                     SPRLTIGEPGGGAASPRCRAIIEMLQASGFTVLASESIQQSIWFKLWGNMTVNPISAL
                     TGATGDRIVGDPLVRAFMSRCMVEARVLGERIGLPIDSTPDARHEVTARLGAFRTSML
                     QDVQAGKATELDALVGAVRELARHEGVETPDIDALFGLARLQAQTLGLYPRLDANPAG
                     A"
     gene            complement(28494..29225)
                     /locus_tag="VGC69_08220"
     CDS             complement(28494..29225)
                     /locus_tag="VGC69_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0295300.1"
                     /translation="MNDQEHSLRVQLAACYRVFAMLGWTELIYNHISLRIPGPDTHFL
                     INPFGLHYTEVTASNLVKIDVRGNVIDAGRWRANPAGFVLHSAIHQGIEAAHCVMHIH
                     TTAGCAVACAKAGLSPDNFYSAQLHGRVAYHDFEGITVHADEGPRVVKSIGDAHAVIL
                     RNHGLLAWGSTLPEAFSYLWTLQRACEIQVLGAALGETLPIPAEIRRQASADALQFDP
                     EYGAGADVFDALVRQVNRIDDSYRL"
     gene            complement(29232..30245)
                     /locus_tag="VGC69_08225"
     CDS             complement(29232..30245)
                     /locus_tag="VGC69_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0295301.1"
                     /translation="MTSYIDVHHHLVPDFYVEAIGADAMHKQAPSYSSVALDWTPESS
                     LQVMDENGIASALLSISAPGLWCGDPAATARLGRRCNDCMAVLIARHPDRFGMFAALP
                     LPDIDATLAEIRYVAETLHADGFGLLSCYGNRHLGDPAFAPVFDALNALGATVFVHPT
                     VTDACRDLMPDFPASIMEFPFDTTRTVLSLLYSGTLTRCPGIRFVFSHAGGAVPFLAH
                     RMARLEQVPAFAAKTQGGALNLLRRHYYDCALSANPPALDALRALVPEDHILYGSDFP
                     FAPGMAGRTRRALDDYDPAGSLLPGIARANALALFPRFAGADEGQRAAAAVIGRSDAR
                     HGG"
     gene            30406..31323
                     /locus_tag="VGC69_08230"
     CDS             30406..31323
                     /locus_tag="VGC69_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295302.1"
                     /translation="MNIRQVDLNLLPVLDALLRHQSVTLAARELDMSQSSLSAALARL
                     RTLLDDELLVRTGHGMQPTARATALAGPLASLLGQLRDDILTTESFAPRKVRRRFSIC
                     LSDVGSYVLWPQIVRAVRAQAPLIELRLRNLSEQALELAIERGEADLIIGAFATPKAS
                     LFQRRLYERAYVCVARAHHPYAGTRMTLRQFANARHIVVRTSARVHEQIDQQLRARHL
                     ERSQITEIPSYLLSPPLLESDDYLAVIPGQLADVFDGRWNLVTLPVPLALPMSTIRMW
                     WHRRANGDPGNLWLRQLIAAELGDGNDPG"
     gene            complement(31336..32298)
                     /locus_tag="VGC69_08235"
     CDS             complement(31336..32298)
                     /locus_tag="VGC69_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295303.1"
                     /translation="MPSLIPLPVPLTSDQSVSAELVAVLVAVSSGEPRVLALHGGTML
                     PAGPFEVGHLSLQGGLRAWVEAQTRHPLGYVEQLYTFSDRDRAAETGVRTISISYLGL
                     TRERQVEAAVPGAWQAWYSYFPWEDRRGSAAPLVDDLVLPRLQAWARRAANAAAQAQR
                     MQRVHMTFGSGGHAWSEDMVLQRYELLFEAGLVAEATRQGRAAHEAGLLPGAAMRYDH
                     RRILATAMARLRAKIKYRPVVFELMPLEFTLSQLQSTVEALAGRGLHKQNFRRLVEQQ
                     SLVEETGAIVAGVAGRPAKLYRFRSDVMLERAISGSKLPLARED"
     gene            complement(32332..33384)
                     /locus_tag="VGC69_08240"
     CDS             complement(32332..33384)
                     /locus_tag="VGC69_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0295304.1"
                     /translation="MVHPSWWAGIVRPGVLAVGMAAVLQGCASSGLDVAAIGNYYVGG
                     HEARLSGLPKTSISTSAGMAPFPYDPNGEFETGQMYVNYTRLSHPKARYPMLMWHGGG
                     MSGVTWQTKPDGKPGWEQYFLGAGYDVYISDSVERGRSGWSQFPQIYASAPIFRAKKE
                     FWELFRIGPMGSYKNSGDKTAYPDSQFPVSDFDVAAMQSTPRWLTNDGITQQAYDEYV
                     QKACPCILVAHSQGTSFAVQAALKAPDKIKALVLVEASSSPDPAKVPVAAMASVPTLY
                     LWGDHIADDPLWPRFQSVSRKFYEALSKVDRNAEWLELPSLGIHGNGHLMMMDRNSDQ
                     IAGIIDGWLASKGMKR"
     gene            complement(33416..34210)
                     /locus_tag="VGC69_08245"
     CDS             complement(33416..34210)
                     /locus_tag="VGC69_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEY0295305.1"
                     /translation="MRVLTVSSWAEFIGLTTELDGWAFRGQADARWPLQSSLSRYLTQ
                     YVEDRGAWGVQEQRAIRIFRRKAHNYISDPGALYDDLRCVGMMQHHGAPTRLLDFTKS
                     PHVAAFFALERALGISSVYALNTPALWLNRAVPTASPQLTRDFIDPRSPGNFEKLFLS
                     NEHSVIWFGEPAEMDQRLIAQAGMFVVPGVIDQTLEEILAGYGGSEELLCKIELPIEL
                     RKSAMRELYRLNITNASLFPDLDGLAHSVALELEMVWPPRRPPDSR"
     gene            complement(34217..36004)
                     /gene="phnN"
                     /locus_tag="VGC69_08250"
     CDS             complement(34217..36004)
                     /gene="phnN"
                     /locus_tag="VGC69_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HEY0295306.1"
                     /translation="MNQARHDMPSGVFFLVVGPSGVGKDTLIDGARQAEPGLLVARRV
                     ITRPAGSPGEDHEAQDEAAFAALASQGAFLVTWGAHGLQYGLRRELLDHLAQGRHVLA
                     NGSRTVIDELARKVPRLVVIEVTAPPELLRDRILSRGRESAEQAGQRLRRRAEVGESA
                     CVLRVMNDGTLEEGVARFLAALRGALAPASSKAAIDAKLSGAALDEDQYAAVIDDIVA
                     GRLDDATIDAFLMHAVDHLADAEVLALAKARTRAMPRIAWNEPIVVDKHSLGGIPGSR
                     ITLIVVPIVAAHGLAMPKTSSRAITSAAGTADAMQTVAKVDLDPQDVRRCIVQARACI
                     AWNGKLNHTLIDDRINARTAALKLQSNRWSVASILSKKYSAGSTHIVVDLPCGPRAKL
                     RTVEEAREMQRLFEEVGAGMGLRVKALVTDGTRPIGAGIGPALEVRDVRRVLSGDPDA
                     PADLLEKALLVAAHILAWAPDVGSLEAGRQRARALLDSGAALAAFERIIDAQGRARPP
                     ILPGRLAYDVCAGRAGVVAQVDGWRITEIAREAGAPRDLSAGVDLLRRPGDAVAAAEP
                     LYRIHADTQAALDAAARSALRHSGVTLQE"
     gene            complement(36005..36838)
                     /gene="nadC"
                     /locus_tag="VGC69_08255"
     CDS             complement(36005..36838)
                     /gene="nadC"
                     /locus_tag="VGC69_08255"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0295307.1"
                     /translation="MYDPFLTDRLIDQWLAEDIGYCDLTAQTMIEPDEQGKFQMNARE
                     PLIVAGIDVATRIFQRYDDSLKIDVKVRDGQFIEKGTVMLVVSGAARSVLTTERTALN
                     IVQRLSGIANLTAQYVKTLEGTKVRLLDTRKTTPGLRMLEKHAVSCGGGLNHRLGLDN
                     GVMIKDNHIVVCGSIAKAVERARRKLPALTKLEVECDRVEQVAEAVEAGVDVIMLDNM
                     SIPEMVKAVALVAGRAKIEASGGISLATIRAIAETGVDYISTSKINQASVCVDIGLDE
                     A"
     gene            complement(36889..37620)
                     /locus_tag="VGC69_08260"
     CDS             complement(36889..37620)
                     /locus_tag="VGC69_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0295308.1"
                     /translation="MKTVSNTLAMAAILLAAWQILHLAAGNGALTSPWDTVRELASLL
                     GSAAFWPHLFETLRALVLALVIGVAGGLALGMALGVHRLAREVAEPILLNLYSLPKVT
                     LYPLVLLVFGLGLPAKVAFGVMHGLIPITVFTMSAIAQMRPVFLRVSATLRLAPMRAM
                     WHIVLPAILPEVVAGLRLGFSLTLLGVLIGEMFASQRGLGYLLTNAMNLGDIKTIMAV
                     ALLLVLFALVCNGLLMRLGRRLSHH"
     gene            complement(37631..38434)
                     /locus_tag="VGC69_08265"
     CDS             complement(37631..38434)
                     /locus_tag="VGC69_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0295309.1"
                     /translation="MATDTASVGAAGVIRHRGLSRASVVRLAVVVLFIAAVELLCRIG
                     IIPLNVMIAPSDMAAHCVELLRAGKFDHDIAISLGNIVSAAILSVLLGFAVGVVLHSA
                     PRVRAAVEPLLSAYYAVPTFVFYPVFIVLLGVGAAPIVAIAVLLAVVSMVTATLTGLD
                     RIPRALHKTALALRLSPWQSAMRVKLPAALPYLFTGVKLSVAYAFIGVIASEFILSGS
                     GIGYAIGYAYNNFENRDMYALMFLVLVLVTLINMALNAVDRRLQARRRR"
     gene            complement(38424..>38991)
                     /locus_tag="VGC69_08270"
     CDS             complement(38424..>38991)
                     /locus_tag="VGC69_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0295310.1"
                     /translation="PDDVGVVFQEDSSFPWLTVRENIGFGLKRKRVPRDEADSRIDAA
                     LDLMSLKDFAAAYPAQLSGGMRQRVCIARTLVLRPSLILLDEPFGALDQQTRLLMGDE
                     LLRLWRETSATVLLITHALDEAAMLSDRVGVMSSRPGLFIDIVETGWERDRDSRIVST
                     PRFGEVNARLWEKLRVESLKVMGIADGH"
BASE COUNT         6770 a        13460 c        12543 g         6218 t
ORIGIN      
        1 gggccagttg gccgacaagc aaccggcccg ctagggcttc gggccgggcc ggcaggccgc
       61 gcggcaacga ggctacagca gcagataggg cgccaaccgc cgcccgtcca gcatgcgcgc
      121 gtggctgaac ggggtcatgt cgatgggcgc cggcttgccc tgcaccaggc tggccatcgc
      181 ccggccgacg cccggcgcca gtccgaaacc atggccactg aagccggacg ccacgtacaa
      241 gcccggtaat gcctggatac ccgacatcac cggcacggca tccggcgtgg aatcgatgca
      301 gcccccccat gccgactgga tggtcgacct ggacaatgcc gggaacatct cgcctgcccg
      361 acgcaccgcg tcgttgagca aatccatatc gggctcggga tcgaggatgc gatgcgacga
      421 aaaaggcgag atgccgcgca ccaggtagtc gcgctccatg cgccactcct cccaaaagcg
      481 cgccaggctc aggctcacgt tcttgcgccg cacctggtag gtgggccaga aacgccatcc
      541 ctggcgcaat agggccggcg tcagataaac cgtgccccgg ccgctcttgg cgacggtaaa
      601 gccgccatcg gtgcgttccc gtaccgcgaa gtacggtgtc gcgatgcaat cttccatgcg
      661 cgtgccgtcg gactgcacgc gcgataccga tccgtacaca taggcctggg gaaacgccac
      721 gcccagcttg cgcaggaaga gcctggtcca tgctccgcct gccacgacaa ccgaagcaca
      781 agcgatgcgc ccccgctcgg tgacgacggc ggcgacgcgg ccattggccg tttccacccc
      841 gtcgacagca cattgctctg ccaccaccgc gcccagcttc tgcgccgcca tcgccatata
      901 gggtgcgacc cgtccaggtt cggcccggcc atcttccgcc gtggtcaggc cccccaccca
      961 ggcgcccgtg gtgccacccg gcatacgccg gcgtgcctgc tcggcactca gcaactcgct
     1021 cttcacgcca tagccggccg ccatctcctt ccaggtgcgc caggcgccaa gctccgcctc
     1081 cgactcggtg agaaaggtga cgccacatgc cctgaagccg acatcgcgct ccagctgcgc
     1141 cgacagttcg ggccagatct gcaggctcag gcgcgccagc tcgagttcct tggggtcgcg
     1201 gccctgctgg cggcaccagc cccagttgcg gctggactgt tcggcgccga ccaggccttt
     1261 ctcgagcagc gcgaccttga cgccggcgcg cgcgagctcc cacgccgtac tgcatcccgc
     1321 gataccgccg cccacgacga cgacgtcgac ctccacgggc agcgtttcgt ccggacggat
     1381 acgttccaga ggctgtctca atgcctggcc caggttcgac ataggttgtc tctcgttctc
     1441 aagggttcaa atggtatttg acgttggccg ccgcgatcag atcgcgcacc acctctgtgc
     1501 ggccgttgtg gcgcagctcg tccggcaagg cttccatctc cctgaagaga tggtgcttga
     1561 ggaaatagcg ccaggtcacc gggcagcgga acaggtagtc cgtcaaggcg atgtagcgct
     1621 cgcgcgtcca gaccttttca tgctcggtgc gggccggcgt gtgcaggaag tgccggcccg
     1681 ggtcgggcac cggcgcctgg gcgcgcaatg cgcgggtttc cgcttcgtcg accttgccgt
     1741 cgcggatgac caccccgtag tcatcgcgcg cctgcccaag gctgacaaag ccccggatgc
     1801 agtcgcgcag cacggcctgc gggtcgcgct ccatgggatt gccatagccg ccgcccgcgc
     1861 atccggtgat gcgcagcaca tcgttcggct cgcaatgcac gatatcggtg ttggcgaggc
     1921 tgacctcccg gtcggtgccg gggttgagca ggaagaacga agccgcgccg gccttgccgc
     1981 ccagcacccc ccaggcgccg aagaacgact tgtcgcggtt gcgggccgtc accatggtgt
     2041 tgggcgcgaa tacctggaac tccatgacca gcgccaggcc gccccggaat ttgcctgggc
     2101 cgccgctgcc gggatagagg tggtactcgc gcatccgtat cgggatttcc gcttcgatga
     2161 tctcgaccgg cgtgttgcgc aggaacgcca tgttggcgcc ggatccctcc acgccgtcct
     2221 cccgcgcgtt gccgcccgcg ccgccgccca ccggccccag cgaggccatg atggtgttgc
     2281 ccgcgtcgtc cgacgtcctg acgttcatga ccgacatgcc cgaagacggc gaggccgaca
     2341 tcttttgcgg caaagccagc gagaacgcgc cgaaggtcac ggactgcatg accaggcaag
     2401 tcaagctgcg catgcccacc gcggccggat gcacggcgtt gaccacgctg cccttgggca
     2461 agatgcagcg cgccacgcgc aacatgccgt aattgagcat catgttggga ttgagcgagt
     2521 acagcacata ggccaatccg accaggggca ggacatggcg ctcacggccg ccggtcggaa
     2581 cgttcaacga cgagttcaac tgcggatcgc tgccggtaaa gtcgaatagc agctcgctgc
     2641 cccgcacctg caggttcagg gcgacgcgca tcggccggcc gtccaccgcg ctgtcctcgt
     2701 cggcatagtc gtcgaagaaa tagtcgccat ccgggatcgc ggccacgatc tccttggcct
     2761 gcatttcggc gtagtccatc agcttttcca cgccgtccat gaattcctcg acgccgagct
     2821 tctcgacgat ctccaggatc ttgcgctcgc ccaccgtcag gctggccatc tgggcgcgca
     2881 tgtcgccgat gttctgctcg ggcagccgca cgttgcaggc catgatgcgc acggcctctt
     2941 cgttgacgcc gcccgcccgg ctcatgatgc gcatcggcgg gaagcggatg ccctcctggt
     3001 gcacctcggt gagcgtacgc gacagcgagg ccggaacggc gccgcccatg tcggtgttgt
     3061 gcacgtggcc gcccacgaag cagataatgc gatcgccgtg gaagaccggc ttccacatga
     3121 cgatgtcggg cgtgtgggta gcgaccgagc cgctgtaggc gtcgttggtc cagtagatat
     3181 cgcccggctc gtattgctcg accgaccgga tcagcggctc gtagtccagg cccgaatacc
     3241 aggtcgcgcc cagcgtgcgg ggcgaggcga aggtctggcc attgaccgac atgatggtgc
     3301 aggaaaaatc ctcggtttcc ttgatgaagg tcgaatgcgc cgtgcggatc agcgcgtagc
     3361 ccatgctctc tgccgcggcg acacagaaat tggcgaagac ctgcaattcc gatgtattca
     3421 tggcgtctgt cctctcaatg ttgctggtat tcgatcagga tgttgccgtg gggatccacg
     3481 acggcctcga acccgggcag gatggaaatc gtggtatcgc tctgcgtgac gatagcgggg
     3541 ccggctatcc ggtgtcccgc gcgcagcgtt tcgcgcccat agaccggcgt atcgcacagg
     3601 ctgccgtcga cgaacagctc ggtccggtag gcgggctgcg cctcggcgtc ggcctgggcg
     3661 atccggggca tgacgggctt ggggttttcg gccgacacca cgactcgcag cgtgatgatc
     3721 tggatgggtg cgtccgtatc gctgtagccg taaatgcgct ggtactgttc gttgaacgaa
     3781 gcgcgcatcc ccgcgatatt caggctggtg acgtcgtcga ccgacaatgc cgtgggcact
     3841 tcgaacgact gccccttgta gcgcatgtcg gcgatggcct cgagcccgta attcgatgcc
     3901 tggccggttt cggattcgat ccagtcggcc gcggttgtcc tcagggccag gaagtcttcc
     3961 ttcaagcgcg gggcatagtc ctcgtccagc ttgtagaaag tcgtcttgac gaaatcgttg
     4021 cgcgtgtcgg cgatcaatcc gcccaaggcg ctgagcaccc ccggcgtatg cggcaccagg
     4081 atgcgcttca tgcggatggc gcgcgcaaaa tggcaggcca tcatggggcc ggcgccgccg
     4141 aaaggcagca gggtgtagtc cttcggatcg atgccgtagc gcgaaatgag ctggctgacc
     4201 ccggcgtaca tgccggagac cgatacgtcg atgatggccg ccgccgtctc gtgcaccgtc
     4261 ttgcccagct tgccggccaa ttcgccgatg gcccgggcgg cgccttcgcg gtcgagcgtg
     4321 acggcgccgt agctcagcgg atggtggccg atatagccca gcaccgcata ggcatcggtg
     4381 atcgtgggcc gcgtgccgcc gcggcggaaa cacaccggcc ccggattgga acccgcgctc
     4441 tcgggcccga ccttgagcac gccgaagctg tccacccagg cgatcgagcc gccgccgtcg
     4501 ccgatggaat tgaccgacac cgaaggaatg aagatctgat actcgccgac atattcgccc
     4561 gtggtgaaat gcggcgtggc attctcgatg atggccacgt cggccgtggt gccgccgatg
     4621 tccaggctca ggcaattggg ctggccggag cgctcggcga tgtaggacgc gccgatcacg
     4681 cctgaagcgg tgcccgacag gatcacctgg atgctgtcgt ccttggcggt gtccgcgcgc
     4741 atgacgccgc cattgctctt ggtgacatgc aggtccgccg gcacgcccag gccgtcgagc
     4801 acgccctgca ggcgagtcag gtagcggttg atgatgggct gcgtataagc ggccacgccc
     4861 gccgtgatgg tgcgctcgaa ctcccgcaca atgggccaga cctcggacga acacagcacg
     4921 aagcggtccg ggtattgcgc catgatggct gccttggcgc tgcgctcgtg ggccggattg
     4981 cggtaggcat gcaaaaagga gatgagaatc ccctcgcact gctcgcgctc gaacaagtcc
     5041 cgcgcggcct cgagcgcctg ctcgacggac aaggggtcga tttcggcgcc gtccggtccc
     5101 atgcggccgt cgatgccgcg cacgcgctcg cgcggcacga tgggcgccgg acgcttggaa
     5161 aacaggtcgt acatgtcggg ggtcttcagc cgcgcaagtt cgaacacatc ctcgaaatgg
     5221 cggttggcta tcaggcccag ccgggacccc ttgccctgca ccaccgcgtt gacgcccacc
     5281 gtcgtgccgt gggtgaagta ctcgatccgg ctcagatcga taccatggtc ttcgcgcagc
     5341 ttggccaggc ccgccttgac gtcgtcgccc ggcgcatcgg cccgggaaaa caccttcagg
     5401 gcgaccaatt cgttggtctc ggtatccagt accgcgaaat cggcgaatga tccgcctatg
     5461 tcgacgccta cacgatagct catggttttt ccgtcaaaat cgggttgaag ggaacaggcg
     5521 ggatgcaacc cgcccaagca atgtatccat ggtcagacgt cccaggccgg cgaccaggcc
     5581 ggatcgaaga gacgcccatc cggcctgcgc aaggcattca aggcccgcat gtcttcgtcg
     5641 tcgagcgtga aatccccggt cccaaggttg agcgccaggc gcccggggtc cgatgaacgc
     5701 ggaatggccg cgacgcccgg ctgctgggcc agccattgca ggacgatctc cgaaggctgg
     5761 cggccatggc gctgcgccag gcccgctatc cgcgtatcgc gcaatagcgc gccgctgccc
     5821 agcggcgcat aggcgacgaa aaccatgtcg tgcccgcggc aaaaatcgag caccgcggac
     5881 tggtccagga aagggtggta ttcgcactgg ttggcgacga tgggctcgcc ggacaccgcc
     5941 agcgcctcgc gcagcagcgc cgtcggaaaa ttggaaacgc cgatatgccg gaccaagcct
     6001 tgcgccttgg cccgggcgaa agccgccagc gtgtccttca gcggcacggc ggggttgggc
     6061 caatggatca gggccaggtc cagataagcg caatccagcc tggccacgct ctcgccgatc
     6121 gatcgcaagg cgtcgtcgta ggccaggtcg gtccaccaga ctttcgacgt gatgaatagc
     6181 tgttcccgtt cgacccggcg cgccttgagc acccggccga tcaacgcctc gttgccgtag
     6241 cgcctggccg tatcgatgtg ccggtagcct gccgccaacg cggcatcgat cgcggcttcg
     6301 ccgcgctcgc catccagttc ccaggttccc aggccgatcc tgggcatggc aaaccgcccg
     6361 tatccgactt gctgcgcatt cgtcatcgcc cgctccgttg tatcgtccgg cggcgtaccg
     6421 gactcgagaa gcacactaaa ataggatttc catgaaaact gtccatcccg cggcgggcgc
     6481 cgccaagccg gactccggca gcgccgaaag cgacggcctc cagcagggcc ctgtcgcctt
     6541 cggcgccaag ctgcgcgccc atcgcaaatc gcgccgcatc accctgctgc aattggccga
     6601 ctgggccgac ctttcgccgg gcctgatcag ccagatcgag cgtggagtaa ccagcccgtc
     6661 gctgcgcacg ctgtccaagc tgcgcaacgc gctggaactg cccaccagct tcttcttcga
     6721 ggaagagccc ggtcacgccg ggtacctgca ggatccgtct tatatctgcc gggccggcga
     6781 ccggccgcgc ctggatctcg gactcggcgc gccgcacaag gagctgctgc accatggcgg
     6841 gtcgcgcgtc ttcgagatga tgatcgtcga gattcctccc gcgggcgact cgggccccaa
     6901 ggtcatcagc tacccatcgg aaaaaggcgg catgattctc caggggcagc tggaactcac
     6961 cgtggatggg cgccgcaacc tgctcgaccc cggcgacagc ttcctgttcg acggcagcct
     7021 gccgcaccaa ctgcgcaacc cgctggacgc gccgacccgc ctgttgtgga tcatcgccaa
     7081 gctgccgaat ctgcatccgt tctgagtcca tggctaaaaa ggcgagtcat tgggcagctt
     7141 ggccacgacc cagatcgtgc gggccatgct gtcggtgggg ttgtaaagaa aatgcggcgc
     7201 gctgccgtcg aacaggaagc tgtcgcccga tgacagcagc gacgatcggc cgtccacatt
     7261 caagtcgacg cggccttcca gcaccaggcc gcccttctcc gagggatatt gcatagccgc
     7321 cggccccact ttggtcctgg gcggaatatg gatgatcatc atctcgaaga cccgcgaatc
     7381 gttgtgatgc agcagctcct tgtgcggcgc catgtcgccc atgtccaggc ggggccggtc
     7441 gcccgcccgg cagagatacg agggatcggc ttcggcttgc ggcaacggcg gatcctcttc
     7501 gaagaagaaa ctgctgggca gatccagggc attgcgcagt ttcaacaggg tcttgagcaa
     7561 ggggctgctg acgccccgct ccacctggct cagcaaaccg ggcgatatct ccgcccgcct
     7621 ggccagctcc tgtaacgtca tatgcttggc cttgcgcacg gctcgcagct tctggccaaa
     7681 cgctgccgca tgcgccggcg gtccggccgg cccgtcgtcg gccccggcgg acgggggggc
     7741 gtcaaggtcc cacgccgcct cgccctcccg cattccgttg ttcgtcatcc gtgcctcgat
     7801 accttgcgct tcaacggccg atctgcttgt tccacatctc gatccatttg ggcgtgttgg
     7861 ccacgatctt gtcggacggg aaccacacgg atttctcggc caccgccacg cgcggctcga
     7921 ccttcgggtc gtatttcacg ttcttgttcg aggcggcata gagcatggcg ttggcgaagc
     7981 acgcctgccc ttcggcgccg gccagatcgt tgacgaaatc ccagcccagg ggagaaccgt
     8041 tcttgacttt ctgcaccagg ttgggaaaag ccacggcgcc cggctcgggg ttgagatagc
     8101 cgatcatcgg ggcgccggcg tcatgctcgg cccaggcccg gccgtcgaag tacatggcaa
     8161 tgtcgatatc gccctggcgc agcgaagcca gcggcgtgtt ggggtcgttg tagaaagtca
     8221 cgttgccgct gtcgcgcatg cgcttgatct ggtccagcgc cggctgcaca ttgtccagat
     8281 cgccgccgta gaccttggcc agcatcgcga tcaggccttg cggcgtcgcg acataagcga
     8341 tgccgggaat cgccgcgtgc cattttccgg cgatggtgcc gtcgacgaat tccttccagg
     8401 tcttgggcgg cgccttcacg gtatcggtgt tgtacatcag gcccagatcg ccgaacgtga
     8461 tgggaaatcc gtagccggcg ccgtagttga gcagggtggg gtttatctgc gtgatgttcg
     8521 gtacgttctt ttccgtcagt tcatcgacca ggccccgctg gcgcgcgatc atgccgctgt
     8581 cgaccgcgcc caccatcaca tcgatgggcg gtttggacgg attggcgctg atctggttga
     8641 tccactgcgt cggcgatccc aggatcacgt ccacggtctt gcccgtgcgc ttctcgaaag
     8701 gctgcacgaa gcacttgcgg taggcctgtt cccaggaacc gccaaacgcc gtgacggtaa
     8761 ggtccgccgc atgggcggcc tgccccgccg acagcgccag cgcgaacgcg gcatatcggg
     8821 gaaatttcat atgcatcggc aatttcattt cactactctc cgcaaggaac gtcacaggga
     8881 ggcgacgggt ctggttgtca gcgcgccgcc tttcggcacc tggcctaatg ctcggtcaag
     8941 gcacgcaggc catagatttt ttccaccacc accagcagca ccacggcaat cagcatctgc
     9001 acggtgctga tcgcggcaat ggaagggtcg aactggtttt ccaggtacga caacatcacg
     9061 actggcagcg tcgatatcct ggcgctgccg aaaaagagcg atagccccag gttgtccatg
     9121 gaaatcagga aagagaacag cgccccggcg aaaatcccgg gcaggatcag cgggaaggtc
     9181 acatggatga aagtcttcca gggattcgcg cccaggaccg aagccgcctc ggcgtagctg
     9241 ccgctcaagc ccttgcagga tgccaccacg gttcgcatga cgtagggcac cgaagtcacc
     9301 gtatgcgtga tcaaaagcgc ggtgaacgac acgcccaggc cgatgtgcga caggaagtac
     9361 aaagaagaca gaccgatgac caagggcggc acggccagcg gcgacaacag gaacgacgac
     9421 gccatgccgg ccaggcgccc gtcgctgcgc gccagcccga gcgcggccgg cacggcgaga
     9481 acggcggcgc agaacgtcga ggccagggcc aggaccaggc tggtgaccag ggaatcggcg
     9541 aaaggccggt attcccagat gcgccggtac cagcgcagcg acacgccgtc gaacgacacg
     9601 gcgaccgcca ggtgcggcgt gaaggacgcg aggaccacga tgacaatggg cgccaggatg
     9661 aacgccacga acaggatcac ataggttccc agggccatgc cgggcaggcg gcgcgccatc
     9721 caggaccata aggccgggct catgccgccc tccccttgcc tatccggctc tgcacgtaaa
     9781 ggcaggccag cacgatgacc agcagcagcg ttcccattgc gctggcgaaa ttcatgtcct
     9841 gcgtctggtt gaactgctgg tagatcagca gtggcagcgt gacgacgctg ctattgccca
     9901 gcagcagcaa ggtgacaaag ctgccgttgg tgatcatgaa cacgatcagg caacccgtga
     9961 tgatgccttc catcgagagc ggcagcgtga ccaggaaaaa agtgcgcatc gcgccggcgc
    10021 ccagcacgcg ggccgattcc tccagcctgc ggtccaccga gcgcagcacc gggatgatgg
    10081 acagcaccat gaaggacacc atcacgtgca ccatgcccgc gtagacgatg gacgccccgt
    10141 ccagcacgcg cagcggctgg ttgatcagcc ccatgcccat cagcgcgacg ttgacgatgc
    10201 cgcggcgcgc gaacagcacg ttccagccga aggtgcgcat gatgatggac gtgagcagcg
    10261 gcatgatgag cacgaacagc agcggcccga cccagcggcc ggcgcggcga acgatgaagt
    10321 acgcaatcgg gtagccggcg agcaggcaga gaaccgtcgt gatcgcgctg actttcaagg
    10381 tttccccgat cagccccagg taataggggt cgaggaacaa cttgcggtag tactgcagcg
    10441 tgaaggccga aggcgtgccc ggaatgctgg atatgccgag gctctgcaac gcgctctcat
    10501 acagcggcag gccaaaaaac accaacagga tgaccagcgc cggcaggtac agccagaacg
    10561 cttgcgaacg ccgccacccg gcccgggccg gcgcggcggc ggtcgttgcc gaggcgctca
    10621 tgcctcatcc tccgggtccg tctcggcatc gaggaccacc acgtcccgca gcttgaacag
    10681 gcagtgcacc tgcgccccgg cctcgcggac ttccgtttcg ccggcgtgcc ggtagacgct
    10741 gatcatcgag cccagcatcg gcacgtcgat ctggtaggca tgctgggcgc cgcgaaagac
    10801 atggttgacg atgcgccccg ggaaacccag ccccgccccc gacgcatcgg ccaggccgat
    10861 cttctccggc cgcagggcca gctcgccttt tctgcggatg cgcacgggcc gttcggtctt
    10921 ggggatgcgc aactcgaacc gaggctgatc ctgcgcgtcg agcaccgcca gcacataggc
    10981 ctgcggctct tcgttcacca gccggtaacc gaggatattg gccgccccca ggaattcggc
    11041 gacaaaacgc gtgcggggat agtcgtagat ttcgcgcggc gggcccactt gcagaatacg
    11101 gccggcgttc atgaccacca cgctatcgct catggtgagg gcttcttcct ggtcgtgagt
    11161 caccatcacg ctggtgatgc ccagcttgcg ctgcaatgcc cgcagctcga actgcatgcc
    11221 ttcgcgcagg ttcttgtcga gcgcccccag gggttcgtcc agcagcagca cggacggatt
    11281 cgtgaccagg gcgcgcgcca gcgcgacgcg ctgttgctgg ccgccggaca actgggaaac
    11341 cttgcgggcg ccgtagtcgt ccagccggat catctgcagc atgcggctga cctgctcggc
    11401 ctgctgcgcc ttgctcgcgc ccgccatgcg caagccgaag gcgatgttct cggccacgtt
    11461 caagtgtgga aacaggctgt agttctggaa caccatgccc aggccgcgct tgttcggcgg
    11521 caggtgggca atgtcggcgc cgccgatgcg gatgctgccg gaatcgggaa actcgaaacc
    11581 cgcgatcagc cgcagcgtcg tcgtcttgcc gcagcccgac ggacccagca gcgacaccag
    11641 ttcgccaggg cgaatgtcca gcgacatgcg ctgcagcgca atcacgccgc catagcgttt
    11701 gctaataccg gccagcgcaa gcggcttggc gggcaaagcg tggcctgcct cgcccaccga
    11761 cgagcgcggc gccgtcaacc accctgaatc cgcagccttc tgcatgacgc aaaccccttg
    11821 cctggcgcat gaggctgccg gcttgcgcgg aaataaagcg cacccaagct catcgccaga
    11881 atttcgttat gttgaaatcc tagcggtaat ttttcacaga ttcacacttt caacccgaaa
    11941 ttcagtgaaa gtacctagaa aatttctaaa ttctataaat ttgtaatgcc ctcacgctat
    12001 cgccaaggga aaatcaatat cctgccgccg gatcgacccg gttcatcagg ggctcgcccg
    12061 cgttcagccg cgcgatattg gcgcagacct gctcagccgt gcatttgaac gaggccacgc
    12121 tggccatgtg cggcgtcacg atgagccccg gcgcgttcca caggggatgg ccgggcgcga
    12181 gcggctcgcg ttcgaacacg tcgaccacgg cgccgcgcag ctggccgctt tccagggcct
    12241 cgaccaggtc ggcctcgacc aggtgctgcc cgcggccgca gtggatcagg gccgcgcccc
    12301 tgggcatggc cgagaaggtg cggcgatcga ggatgccgcg ggtctgctcg gtaagcggga
    12361 tcaggcacac cagcgcgtcg agcccggaca ggaaggtctc gaaccggtcg ggcccggcat
    12421 ggacctgcac gccggcgagt tcccgggccg tgcgcgccca gccgctgacg cggtagccga
    12481 agcgggccag gccctgcgcc acgtcctggc ccagccggcc caggcccatg atgcccacgt
    12541 ggaagtccga tgccggcggt tgctccggcc gcgtccagcc atgccgggcc tggtcggcca
    12601 gcacgccgtc caggccgcga tggaaggtga tcacgctcca gaggatgaac tgcagcatcc
    12661 cctcggactg ctgcgggtcc accacccggc agaccgggat gtgatgcagc gaggcatcgc
    12721 ccaggatatt gtcgaccccg gccgcgatgc tgtggatgag ccgcagcctg ggcatttttt
    12781 cgtaggcccc cggcggggtg tgccagcaga cggccacttc ggcctccagg caccgcgggt
    12841 cgggccaggc atacagttcc agatcgggat cgaccgcttt cagcaaggga gtgaacagag
    12901 cgcctaggtc gaaggtttgg ctcatcaagg caacgcgggt catgggcggg gagatagaag
    12961 gcgatggata gggaaaacga gggggaaacc ggctacagca gctgcgcgct cttgagatct
    13021 tccatgaagg cgttgagcaa gtcgtcgccg gcctgcgccc ggctggtcac gacgtggatc
    13081 gtcacttcgt aggacaattc cagcggattc aaggccgcca tcaggccccg gtcgacatag
    13141 ctttgcccga tttcctgcgg caggtagccc agatggcagc ccgatagaat caggatgctc
    13201 atggcttcca tattacccgt gacggccgtc acctggcggc gcggcaccgg cgtcatgccg
    13261 gccggcacgg gatagccgcg ccaggcccac tggtgcgtca tggcatcctc cggcgacacg
    13321 ctgccggcct gggcgaataa aggatgggac gccgcgcaat aggccacctg ccgctccagc
    13381 atcaagggcg tgtactgcag cgccggcacg cggcgccaga agtagccgac ggccacctgc
    13441 accgcgccgt tgagcagcat gtcctccagt tcgcccggcg tgcgcaccac caggtccagg
    13501 cgcaccgcct cgtcgcggtc gcgaaaacgc cggatggcgt cgctcagccg cgcattctcg
    13561 tgcgagggcg tgtggcccat caggccgatg ctcaggctgc ccaccagctt tttgtcgagg
    13621 ttgcgcgcct cgatgccgaa ctcggtgagc gcgtcgagca tgcgccgcgc cgcatcggcg
    13681 aatttctcgc ccttggcggt cagccggaag ccggcgcggc cgcgctcgca cagcttgtag
    13741 cccaggcgcg tctccagcgt ggccagctgc gtgctgatgg tcgactggcc cacattgagc
    13801 ttggcctgtg cggcggagac cccgtaggcg tccaccacgg ccagaaaaac ccggatcaaa
    13861 cgcagatcca ggtcagaaac attgctcaac atagtaataa cccgaatttt ttactggaca
    13921 tacatcaatg attatcgatg caaatgtcgc cacgtaccta tttttcttta tctgggttgg
    13981 tcctagactt ccccgcttta cgcctcttga agaacccccg ccgccatgag tgaaaaactt
    14041 ctgtggggcg cgcgcttccg cgccgcgccc gacgccgccc tgatgaacct gtcgcgcagc
    14101 gacgccagcc acttccgcct ggccgcctac gacatcatgg cctcgcaggc ccatgcccgc
    14161 gaactcgagc gtgccggcct gctcaccgcc gccgagcgcg aagccatcgt cgccacgctg
    14221 ggcagcctca aggagcgcat cgcgcgcggc gagctcgcgc ccgtcgccgg cgacgaggac
    14281 gtgcacacct tcatggaacg cctgctcgtc gccgaactgg gcgacaccgg cgccaagctg
    14341 cgcgccggcc gctcgcgcaa cgaccaggcc gccaacaacc tcaagatgta cttgcgcgac
    14401 gaggcccgcg cgctggtcgc cgagctgctg gcgctgcagg acgccctggt cgggcaggcc
    14461 ggcaagcacg cgcagaccct ggcccccggc ttcacgcatc tgcaggccgc gcagcccatc
    14521 gtcttcggcc accagctgct ggcgcatgcg cagaccttca accgcgacgc ctcgcgcttc
    14581 atcgactggg atcgacgcag cagccgttcg cccctgggcg gcgcggccat ggccggctcg
    14641 gccatcgccc tgcatcccga gctgtcggcc gccgacctgg gctacgaagc gccctgcgag
    14701 aattccatcg acgccatcgg cagccgcgac cacgtggccg agttcctgtt cgtgtcggcc
    14761 atgctgggcg tgcacctgtc gcggctctcg gaagagatca ccctgtgggc ctcgcgccag
    14821 ttccaatggg tcaagctcga cgacgccttc tccaccggca gttccatcat gccgcagaag
    14881 aagaaccccg acgtcgccga actgacccgc ggccgcgccg cccgcctgat cggcatgctg
    14941 accaccatgc tggccgcgct caagggcctg cccttctcgt acaaccgcga cctggccgaa
    15001 gacaagtaca ccgccttcga ggccgtggac gtgctgcaca aggtgctgcc cgccatggcc
    15061 ggcctgatcc gcaccctgaa cgtggacgtc gagcgcctgc gccgccaggc cgtcgagggc
    15121 tacacgctgg ccaccgaagt cgccgactgg ctgtcgcgcc gcggcgtgcc gttcgccacc
    15181 gcgcacgaga tcaccggcgc gctggtcaag cgctgcgagg acctcggcat cgagctgtcc
    15241 gagatcagcg acgccgacct ggtcgccgtg gacgcccgcc tggatcccgc catccgcgcc
    15301 gtgctcacgc ccgaggccgc cgtggcggcc cgctccggct acggcggcac cgcgccggcc
    15361 cgcgtggccg agcagatcga gcgcctgaag gcggtactgg acaagcaggc cgaatgggcc
    15421 cgcgccgggg tcgcgcgatg ctgaagaccg gggccggcga ccccgcgcgc ggtccggcga
    15481 cgccggccga cgggatctcc cattaccagc acgtgccgcg gcgctactgg ggccgctacc
    15541 tctcggccgc cctcatcgtg ctggccatcc tgttcgtgct caacgccttc gcgcacggcc
    15601 agatcgagtg gtcctacgtg ggtcgcttct tcaccgcccc gaccatcctg cgcgggctgg
    15661 gcaacaccat cgtcatgagc atcgcggcga tggcgctggg cgtggccctg ggcatcatca
    15721 cggccgtgat gcggctgtcg cccaaccctg tgctgagcac ggtcgcgcgg ggctacgtct
    15781 ggctgttccg cggctcgccg gtcatcctgc agctgctgct gtggttcaac ctggccctgg
    15841 tgttcccgac gctcggcatc ccgggcctct tcgaggccaa gacggtcgac gtgatgacgc
    15901 ccttcctcgc cgccctgctc ggcctgggca tcaaccaggg ggcctatacc tccgaggtgg
    15961 tgcgcgccgg cctgctgtcg gtcgacgtcg gccagtacga ggcggccaag gccatcggca
    16021 tgccgcgcct gcaggccctg cgccgcatca tcctgcccca ggcgatgcgc gtgatcatcc
    16081 cgccgatcgg caacgagttc gtcggcatgg tcaagctcac ctcgctggcc agcgtgatcc
    16141 agtacgccga agtcctgcac aacgcgcaga acatctacta cgcgaacacg cgggtcatcg
    16201 agctgctgat cgtcgccggc atctggtacg tggccgtggt caccgtgctg tccctgctgc
    16261 agggccgcac cgaagccttc ttttcccggg gctccggccg gaaatccgcg cgccgatgat
    16321 gcccttccac ccctattcca aaccgctcgg gaacgccacg acatgagcac catcgtgaaa
    16381 gcggtcgaca tccgcaaggc atacggcgac ttcgtcgcgc tgcacggcat cacgctggag
    16441 gtcgagcgcg gcgaagtgct gtgcatcatc ggcccctcgg ggtcgggcaa aagcaccttc
    16501 ctgcgctgca tcaaccagct cgaggaaatc agcggcggcg ccctctgggt cgacgaggaa
    16561 ctggtcggct accgcatctc gggcaccaag ctgcacccgc tctccgagaa agaagtcgcg
    16621 cgccagcgcc tgaagacggg catggtgttc cagcgcttca acctgttccc gcacatgacc
    16681 gtgctggaga acgtgatcga gggaccggcg cgcgtgctgc gccagccgcg cgcgcaggcc
    16741 ctcgagcagg cccaggcgct gctcgagcgg gtcggcctgg ccgccaagaa ggacgcctac
    16801 cccatcgaac tctccggcgg ccagcagcag cgcgtggcca tcgcgcgcgc cctggccatg
    16861 cagccgcagc tgatgctgtt cgacgaaccc acctccgcgc tcgaccccga actggtcggc
    16921 gaggtgctgg ccgtcatgcg cgacgtggcg cgcaccggca tgaccatgat cgtcgtgacc
    16981 cacgaattga gttttgcgcg cgaggtcgcc gaccgcatcg ttttcatgga ccagggccgc
    17041 gtcgtcgagt ccggcgaccc ccgctccctg ctctccaatc cgcagcacgc gcgcacgcgc
    17101 gaattcattt ccgccgttct ttcctgatca ctctaccagg ccattaggag ccatcgatga
    17161 agacgtcctc cggcaagtcc ctctttaccc gcgccgcgct cgcctgcgcc ctctcgaccg
    17221 ccgccctgat gtccgcccag gcccagggcg ttcccaagga actcgcggcc gccatcgtgc
    17281 ccaattaccc gccgctggac tacaaggatc cggccaccga cgagctgacc ggtttcgacg
    17341 tcgacctcgg caaagcgctg gccgccaagg ccggcaccca gttgcgctgg gaagaaacca
    17401 gcttcgacca gatgatgagc gccgtggcca ccggccgcgt caacatcatc ctctccggca
    17461 tgaccgacct ggcctcgcgc cacgacgccg tgcacttcgt cgactacctc aagacgggtc
    17521 cgcagttcta cgtcatgaag gcgaaccagg cgcaataccc cgatatgcag gcgatgtgcg
    17581 gcaagaaggt cggctccagc cgccgcacct ctttccccaa agagatcgcc caatggagcg
    17641 acgacaactg cgtcaaggcc ggcaagcccg ccatcgtcgt ggtgggcacc gacggctcgg
    17701 ccgacgccca gacgcagttg cgccaaggcc gactggacgc cgcggtgcaa ggcggcgaga
    17761 ccctgcccta cctccagcag caagcgcccg gcacgtatac ggtggtcggc aagcccatcg
    17821 ccttccagta cgtcgggctg gccgtcggcc tcaaggacga ggccctgttc aaggcgctga
    17881 gcgacgccat ggcggcgatc atcgccgacg gcacgtacgc caagatcacc gccaaatggc
    17941 acctggagga cttcgccgtc tccaagctga tcaccgacag cgccgaataa gcgccaggag
    18001 catttcagcc atgcagtcca tcgatcccgg ccggccgacc tggcccgacg ggaggcagtc
    18061 ctgcgtcgcg ctcgccttcg acctcgacgg accgaccggc ggcgcgatgc tggacggctc
    18121 gctctggcac aacccgtcgc tcttcatgct gggcgcctac gagccctggc gcgcgctggg
    18181 ctacctgctg ggcctgctgc gcgaactgcg cacgccggcc accttcttcg tgcccgcctg
    18241 ggtgctggac acctggcccg agcaatgccg ggccatcgtc gaggacggcc atgaagtggc
    18301 ctaccacggc gaccagcacg aggccttctg gcaactcgac gccggccagc agcgcgaggt
    18361 catggcgcgc tcgaaggccg tcttcgcccg gcacctgggc cttgcgccca cgggctttcg
    18421 cactccctcg ggcgactggg ggccggacac cgtcgacatc ctgctcgagg ccggcgtgcg
    18481 ctattcgagc tccatgcgcg gcgacgaccg gccctacttc atccccggca cggacggcgc
    18541 gcgcggcctg gtcgagatcc ccggccgctg ggagatggac gactacacct cgctcgccta
    18601 tcagcgccgc cccgactacc ccgcggggct ggaccgcatt gccggctacg acaacacgct
    18661 cgacaactgg cggcgcgaat tcgacggcgc gcacgccgag ggcttgtgcc tgaccacgct
    18721 cttgcatccc aaggtctcct gcaaaccggg ccggctgggc atcctggagc ggctgatcgg
    18781 acacatgcgg gaccagggca atgtgtggtt cgccacctgc gcccaggtcg aatgctggtg
    18841 gcgcgaccga accgcgaagg aaacaccgtg aacggcctgt ggcccgaaaa ccgccgcagc
    18901 gcggcgaccc tcaccgtcga cttcttcgac gacgccttca tcctggcgca ggagccccgc
    18961 atggccgggc gcgacaagag cctgtcggta tggcgctacg gcgcccagcg cggcgtcgaa
    19021 cggctgctgc ggctgttcga cgacatggcc gtgcgcacca cctggttcgt gcccggctgc
    19081 gtcgccgagc ggcatgccgc gctggtgcgc gacatccacg ccgccggcca cgagatcggc
    19141 aacgccacct acgcctgcga aaacttcgac gcgctgccgc tcgaggcgca ggccgatagc
    19201 gcgcggcgcg cccaggacgc catcggccag gccatcgggc aggcgcccga ggcctttcgc
    19261 tcgccctggg gcaattgggc gccgggcctg ccggtcgcgc tgctgcgggc gggactgcgc
    19321 tggagctcgt cctggcgggg cgacgaccgg ccgtatttcc acgccggcga acccggcgcg
    19381 caggacaggc tgctggaaat ccccctgcac tacgagctcg aggacgagcc ctacttcatc
    19441 ttcaacctgt acccgccggt gccctcgggc cagccacgca tcgcgcccta ccgcgacgcg
    19501 ctgtcgaacc tgatgcagga tttcgcgggc tttcacaagc tgggcctgtg ctacccgctg
    19561 cgcatccacc ctgagatcct cggcaccgcg gggcgcatcg gcctcttgcg cgaactgctc
    19621 gagcacatct cggccacgcc cggcgcctgg ctcgccagcg gcggcgagat cgcggcctgg
    19681 tggcgcagcc gccacaccag caacgagccc ggccacccgg ccgacctctt cgcgccgcgc
    19741 caccggccgg cgcccacgcc atgacagccc ccacctacgc ccggcaactc gcccgattcg
    19801 cgaccgccac cgcattcgcc gacctgccgg cgccgctggt cgaacgcgcc aagcggcaca
    19861 tcctggacac gctcggcgcc gccctggccg gcagcgcttc gaccgaagcg cggacggtcc
    19921 aggcggtgct gcgccaggat gccgccggca gcggccccgc ccgcatctgg ggcctggacc
    19981 gcgcgatcgg gcagcgcgac gcggccttcg ccaacggcgt cgccgcgcat gcgctggagc
    20041 tggacgatgc cggcggctgc gaccacagcg gcgccgtcgt gctgcccgcc gtgctggccg
    20101 cggcggcggg cgcgcccgag ccggtttccg ggcaggactt catcgccgcc gtggtgatcg
    20161 gctacgacgt gggccggcgc gtgctcgagg cctgcggcgg ctatcccgcg cacaacgggg
    20221 tcggctggca ctccaccgcc acctgcggca ccttcggcgc ggcggcggcc gtcgcgcgcc
    20281 tctggcgcct ggacgagcag caatgcgcct gggccctggg gcatgccgcg agcttcagcg
    20341 gcggcctctg ggccttcatc cacgacggca cgcagaccaa gaagctgcac accggccgcg
    20401 ccgccgaggg cggcctgctc gccgcgcgga tggccacggc cggcatctcc ggcccggcgg
    20461 ccgtgttcga ggacgtctgg ggcggattct tccgcgcctt tgccccgcag acggcgcagc
    20521 ccgaagcgct gaccgccgag ctcggcgaga cctggaagct gctgcgctgc tcgatcaagc
    20581 cgtatgcggc gtgccgcagc acccacgcat ccgtcgacgc cctgctgcac ctgctcgcca
    20641 acgccgaggc cgggcgccag gccgccgccg tcgacgtccg gctcagccaa ttcgtaatga
    20701 acatgtgcgg cggacgcgac ctcaccacgc tggccagcgc ccagatgagc atgccctatg
    20761 cgcttgccgc cgcctgggtc ttcggggatg ccaatatcga gcgctatctg cagggcatgc
    20821 gcggcgaccc ccggcttgcc caggccatgg cgcgcatccg cctgcacccg gatcccgcga
    20881 tggccgacct cgaagagccc accatcacgc tctcgggcga cggcatggcg cccgtttcgc
    20941 atcacgttcc cattgcgctg ggcgcccccg ccaatcccct gtccgatgcg cagctgctgg
    21001 ccaaattcac gcagctggcc tgcgtggcgc tgccgccctc gcaagccgaa cagctggccg
    21061 accgcgtact gcggctggaa gccgacccgg acagcaggac gctgcaagac ctcctgctcg
    21121 gccatgccga tgccgcccgg ctgatcgcct gagcggccgg gccgcgtata cactaaaggg
    21181 cgtgaaaacc atcatcggcc gccctggcgg ccagcacgaa ttgcaaggga aacactaagg
    21241 gtgaaatgat atggataatc cgctgaacgt catcgacagc gagtggacaa cccgctgcga
    21301 actcgccgcg ctctatcggc tggtcgcgca ctttcgcatg accgatatga tcgacacgca
    21361 catctccgcg cgcgtgccgg gccaccagga ccagttcctc atcaaccgct acggcgtgct
    21421 cttccatgaa atgcgcgcca gcgacctggt caagatcgac caccagggca aggtcatcga
    21481 gaccggccgc tcggcggaag aaacgcgcgt caacgcggcc ggctttacga tccactcggc
    21541 cgtgcacatg gcgcgccacg acctcaactt cgtgatccac acccacaccg ccgccggcat
    21601 cgccgtgtcg gcgcaggagc acggcctgct gcccatcagc cagcacgcgc tgcgcttcta
    21661 cggcaagctc agctatcacg actaccaggg catcgccctc gacctcgaag agcgcgagag
    21721 cctcgtcgcc gacctcggcc ccaccaacaa ggccatgatc ctgcgcaacc atggcctgct
    21781 gggcgcaggc cgcaccgcct ccggcgcctt ccaggaaatc tacttcctcg aacggtcctg
    21841 ccaggcccag gtggccgcgc tgtcgggcag ccagcaactg cgctacccca gcgagacggt
    21901 ctgccagcgc acggccggcc agtacaaggg cgaagagacg ggcttctatg gcgacatctt
    21961 ctggtcgggc tgcctgcgcc tgatcgagaa cgaccagccc gactaccgga gctgatccgg
    22021 gagccccccc cgtggctagg cggcgggagc cagaggccgg cctcgcgcct gcgcctggcg
    22081 ccgctcgagc atgcggtcga aggcatcgac aatgcgctgc acataggggt tcttgaaccg
    22141 cagcgcggcg tcgccctctt cttccggatg gaccagcatg gtggcattgg cctcgaacac
    22201 cagcagccgg ccgtccggca gcacggcaaa atcgaccccg cagtaatcca ggcccaggcg
    22261 ccgcccgatc tcgcgcaggg cctgccaggc ccgcgtgccc agcacggctt cggggtcgtc
    22321 cagaaagcgg cgctcttctt cggttttcca gggcgcggcc gtcatgtcgg ccgtcacgta
    22381 gtgcagcacc catcggtcgc ccacggccag gtgataaggc cagggctcgc catcgaccag
    22441 gacgacccgg tacttgcgaa agcacccgtc gggcgagcga tagtcgcaat attggctcag
    22501 gtagaggtcg ccgtccgcgc gcgggtgggc ggcggcatcc tgcggcgtgt ccagccgctc
    22561 caggccctgg ccgccgtgcc ggcccaccgg ccggacggtc acggggtaag ccaggccggc
    22621 ccgctcgatg gcttgcggca agcccaggcc ttgcgccggc cgatgccgca cggcaggcgg
    22681 catgcagacc ccatcgatgt ccgccagcag ccccggcatc cggtcgcgcg aggtcagcgt
    22741 gatgcgcgca ggcaagttca gcacgggctt gtcgatgtcc gcgatgtagt tcgccaccgc
    22801 atcatggctg gcctgcgcgc tgtcggcctc gccgacgacg ttgaacacca ggtcgtgggg
    22861 cggcagctcg cgcaccggcc cgaccaggcc gtattcgatc gcccagcgga tctggcgatt
    22921 gcgctgcttg ggcagcagga actgcaccgg cacattgtcg ttgcggctgg tgcacagcac
    22981 gagcacgctg cgccgctggg tcggcagggc ctcgtcgatg aacagggact gccgcaggca
    23041 ggcattgtgc gagtaggact cggactcggc caggtggccc gtctcgcgca aggccatgcc
    23101 catgtacagg taggcataga ccatgtccgg gtccagcagc agcgcccgct ggaaccagta
    23161 ccccgccgcc gcccactgat tgtggcgggc gtagctgcgg cctatgccgt agaggtcttc
    23221 ggccgatttc cgttcgagcg cttcgcaggc cagccgggcg gacaaggcgt cggccgcctg
    23281 gcccatggcc tccatggcct gcgcgagcca gcggtagatc tcggggttca ggggctgcgc
    23341 gtgggaggcc tgctgcaaag cctcgacggc cggcatgtgg cggccgccgg ccagatgcgc
    23401 cctgccctgg gccagccagg cctgcgccgc ctcgccgttc actgcccctt gcccgcttcg
    23461 atcgcctcga gccgggcctg caattgctgc aactgctcgg cgagctggtc caccttggac
    23521 cgggcgcggg tcagcatgtc ggcctgcacg tcgaattcct cgcgcgtgac caggtccagg
    23581 cgcgaaaacg cctgggtcat catcgaacgg atattgcgct cgatatccgc cgccgggctc
    23641 ttggcgatga gctcggaaat gttcttctgc agatcttcca tccattgcgt acggttcatg
    23701 gctacctcgt gccgactttg ctgatggcgc cagtgtagac ccgccaaggg ggaagcttgc
    23761 tgttcccggc cggcgccaaa aaagaagcgg cccgccaagg gagcgggccg caaaggcgca
    23821 ttgccgaacg cctgcctgga ttgttcacga atttcttgca ctgcacaaac ccgagcttgt
    23881 aaatcggcgt atccgattct gccggagcca tcccgggcct tgccccttga accgctttcg
    23941 aaatacattg caatttgcta caaattttcc gatacgatcg attgcttgac ctcggtcttt
    24001 tgaatgccca atatcagtgc aacctatccg ggcataaatg caccaatatt gggctattaa
    24061 cgcaccaata tggaatctca tggacagatc agaatgatgc gaaacggttt tttagatggc
    24121 atatttcttg ctaggtacac gatgcctacc gcgcaatcat cagaggaata gagccatgaa
    24181 actcatcatt gccatcatca agcccttcaa gctcgacgaa gtgcgggtgg ccctgtccgg
    24241 tatcggcgtc caaggcctga ccgtcaccga ggtcaagggc ttcggccgcc agaaaggcca
    24301 caccgaactg tatcgtggcg ccgaatatgc ggtcgacttc ctgcccaagc tgcgcgtgga
    24361 agcggccgtg cccgaccatc tcgtcgacca ggtcgtcgaa gccatcgaac aggcggcccg
    24421 caccggcaag atcggcgacg gcaagatctt taccgccccg ctcgaacaag tcatccgcat
    24481 ccgcacgggc gagtccggcg aagcggctct gtaaaaacac aaacaacatc aataaggtac
    24541 tggagctcca cacaaatgga taaagcagat atttcctgga tgctggtctc gaccctgctc
    24601 gtgctgatga tggccgtgcc gggcctcgcc cttttctatg gcggcctggt ccgcagcaag
    24661 aacatgctgt ccgtgctgat gcaggtcacg ggtgccttca cggtgggcct gatcctctgg
    24721 ttcatctacg gctactccct ggccttcacc gacggcaact cgttcttcgg cggattctcg
    24781 cgcatgttct tctcgggcat gttcaatccg gccgacggca agtacgccat gaacggctcg
    24841 atcacggaaa tcctgtatgc gtctttccaa gccacgttcg ccggcatcac ctgcgccctg
    24901 atcgtgggca gcttcgccga gcgcgcccgc ttctcggccg tgctggtgtt cacggtgatc
    24961 tggttcacct tcgcctacgt gccgatcgcg cacatggtgt ggttcgccat cccctcgggc
    25021 cagcaaggca cctccggcct gctcgcggcc gcgggcgccc tggacttcgc cggcggcacc
    25081 gtggtgcaca tcaatgccgg tatcgccggc ctggtgggcg cctatgtgat cggcaagcgc
    25141 gtgggctacg gccgcgaatc catgcagccg cacaacctgg tcatgacctt catcggcgcc
    25201 atgctgctgt gggtgggctg gttcggcttc aacgccggct cggccgtggc cgccaatgag
    25261 ctggccacca tggccttctt caacaccatg atcgccaccg ccgccgcctc gctggcctgg
    25321 ctgttcaccg agtgggccgc caagggcaag ccctcgatgc tgggcgccgc ctccggcgcc
    25381 atcgccggcc tggtgggcat caccccggcc gccggcttcg tcggccccgt gggcgccttc
    25441 gtcatcggca tcgtcgtggg catcatctgc gtgtggggcg tcaacggcct caagcgcctg
    25501 ctgcgcgccg acgacgcgct ggacgtgttc ggcgtgcacg gcgtgggcgg catcctgggc
    25561 gccctgctga ccggcgtgtt cagcgccaag ggcctgggcg gcctgagcct ggacaccatg
    25621 gacgccatcg gcggccaggt gctgacccag ctcgaagcca tcgtgatcac cctcgtgtgg
    25681 tccggtgtcg tcgcctggat cgcctacaag atcgccgaca tgctttgcgg cctgcgtgtg
    25741 ccggaagacc aggaacgcga aggcctggac gtcaccacgc acggcgaatc ggcctaccac
    25801 agctaaaaga aaaggggcta tgcacggccg cccggcctgc cgggcaggcc gccccaaggg
    25861 gaaacggcgc cgggcgtgaa aacccgggcg ccgttttttt atggtgagct catcctatgc
    25921 cggtgctttc cacaccccag cacgcgaccg tcaggccagt gccgaagcat tggcatgaat
    25981 cacccgcttg cgatcgatca ggcagaacac caccacgaac aaggcggccg acgcgggaaa
    26041 catcgccatc gcgaaaaaca agtgctccga accgaatccg ctcgagatca gcaggctgat
    26101 gacaagcggc gccacaacct gcccggtgcg ggtgctggcc atgccgaagc cgatgccggt
    26161 cgcccggata tcgatgggat agagcaaggt catcaccgcg atcgcgccgg cacaccccat
    26221 gccgcaggtc aggcccagca ccgtggccgc ggcggcggcc ccagccacgc tccccgcggc
    26281 cggcaggctc gccaccgcgc atgccgcgag aatgaatggc acgaacaagg tgcgcagcgg
    26341 cccgaatcgg tcgaccaaat agccggccgc ccccattccc acgaagcagc ccgcattgaa
    26401 gcccgccagg atcgccggga ccgcccggtc gcctatgccc ttgtcgtgaa tcaggatcgg
    26461 cgtccaggtc acaaccaggc tcagcgtcgc gaacgaaacg gcgagaatcg cccacagcac
    26521 gaaggtcgac gccttgcgat ggtcgccgaa cacttctctg atcgagccat gccggcgctt
    26581 gcgctcatcg cacacgaagc gctcgacgga cggcggcacc gatatcccaa gccatttcaa
    26641 tcgggcccgg atcagcgacg gcgcggcccc cttggcagcg agatgctgca ccgtctccgg
    26701 caccaggacg aacagtacca gggcaatgca gatcgggatg ccgccaccga ccaggaacac
    26761 cgactgccag ccccacctgg ccagcatcgc cgagttgagc agcccgccca gcatcccgcc
    26821 cagcggaaag aacgaccagg cgaccgccac caaggtgccg cgccggcgca gcggcgcata
    26881 gtccgacacc atggacagaa aacagggtgt cgcgccgccg atcccgatgc cggcgagaaa
    26941 gcgcagcacg gtcagcgacg tcaggctgtc ggcccaggcg gtcgcgatcg agaacacgcc
    27001 gaggatcagc agcgcgataa gcagcgtcga cttgcggccc catcggtcgc ccagggagcc
    27061 gatcgtcagc gcgccgagcg ccgccccgag atgggtgatc gagaacacga tgccaaatgc
    27121 ctgcgcatga atatcgagcg atcccatgat cgagcgcgcg gcgatgccga tcacctggct
    27181 atcggcgccg tcgagcaaac tgaccagcgc gcacagcatg accaccaggc cgtgccgcca
    27241 ggtaaacgcg gcgttgtcga tcagccacga tacattgaca acgccgttgc tctgcgggct
    27301 ttcctgggtg ctgccggcgc tgcggcccaa ttgattgcct gtctccactg tcatcatttt
    27361 tttatctcct ccaccacctg ggcttgccct tgcggactgc cctattcgcg ctgtacacgc
    27421 cgccggcaag ccagcccggc cgccatgtcc tgcgcgtcct gtttgtcggc atccatcaag
    27481 cgcccgccgg atttgcgtcg agccgcggat acagccccag ggtttgcgcc tgcaggcgcg
    27541 ccaatccgaa cagcgcatcg atatccggcg tctccacgcc ctcgtgccgc gccagctcgc
    27601 gcaccgcgcc gaccagcgca tcgagctccg tcgccttgcc ggcctggacg tcctgcagca
    27661 tcgaggtgcg gaaagcgccg agccgcgcgg tgacttcatg cctggcatcg ggcgtcgaat
    27721 cgatcggcag cccgatccgt tccccgagca cgcgggcctc caccatgcag cggctcatga
    27781 atgccctgac cagcggatcg ccgacgatcc ggtcgccggt cgcgccagtg agtgccgaga
    27841 tcgggttgac ggtcatgttg ccccacagct tgaaccagat cgactgctgg atcgactcgc
    27901 tggcgagcac cgtgaaaccc gaggcttgca gcatctcgat gatagccctg cagcgcggcg
    27961 aggccgctcc gccgcccggc tcgccgatgg tcaggcgggg cgacggcagg tacgcgacct
    28021 cccctggcgc aggcgtgctc gccgacagat tgctgacgca gcccaccacc cgctcgggcg
    28081 cgatcgccgc actgattgcg ccggccgggt cgacggtctc gatccgcctg cccggaccga
    28141 cttcccggat gccgtgatag aaccaccatg gcacgccatt catcgccgac agcaccaccg
    28201 tgtgctcgcc cagcaagggc gcgatgcgtc gcgcgacatc gccgatcccg gtcgccttga
    28261 cggcgatcac gaccaggtcc tgcgccccaa gcgcggccgg attctcggcc acccgaagcg
    28321 atgcgtgttc ggtgtggccc gcgctcgtca aggcgagccc cgcgcgattg acgtgttcca
    28381 atacgcggcc ccgcgcgacg gcgcatacgt cgacgccggc cgatcgcaat ttgccggcga
    28441 tcagcccacc caccgcgccg aggccataga cgcagacttt cattgccatt gcctcacagc
    28501 cggtacgaat cgtcgatccg gttcacctgc cggaccaatg cgtcgaacac gtcggcgccc
    28561 gcgccgtatt cgggatcgaa ttgcaaggcg tcggcgctgg cctgccgccg aatctcagcc
    28621 ggaatcggca aggtctcgcc cagggcggcg cccagcacct ggatctcgca ggcccgctgc
    28681 agggtccata gatacgaaaa cgcctcgggc aatgtcgatc cccacgccag caacccgtga
    28741 ttgcgcagga tgacggcgtg agcgtcgccg atcgatttca ccactcgcgg cccttcgtcg
    28801 gcgtggaccg tgatgccctc gaagtcgtga taggcgaccc ggccatgcaa ctgcgcgctg
    28861 tagaaattgt ccggagagag ccccgccttg gcgcacgcca ccgcacagcc cgccgtcgta
    28921 tggatatgca tcacacagtg cgcagcctcg atgccctgat ggatcgcgga gtgcagcacg
    28981 aatcccgcgg gattcgcgcg ccagcggccg gcgtcgatca cgtttccccg cacgtcgatc
    29041 ttgaccagat tcgacgcggt cacctccgta tagtgcaagc cgaacggatt gatcaggaaa
    29101 tgcgtgtcgg gaccgggaat ccgcagggaa atgtggttat agatcagttc ggtccagccc
    29161 agcatcgcaa acacgcgata gcaggccgcc agttgaaccc gcagggaatg ctcttggtcg
    29221 ttcatcgatg ttcacccgcc atgccttgcg tcggaacggc caatgaccgc ggccgcggcg
    29281 cgctgccctt cgtcggcgcc cgcgaagcgc ggaaacaggg ccagtgcatt ggcgcgcgcg
    29341 atgccgggca ggaggctgcc ggccggatcg taatcgtcga gcgcgcgccg ggttcggcct
    29401 gccatgccgg gagcgaaggg aaaatcgctg ccatacagga tgtggtcctc gggcaccagc
    29461 gcgcgcaatg cgtcgagcgc cggcggattg gccgagagcg cgcagtcgta gtaatggcgc
    29521 cgcagcaggt tcagggcgcc gccctgggtt tttgccgcga aggccggcac ctgttccagc
    29581 cgggccatcc gatgcgccag aaacggcacc gccccgcccg cgtgggaaaa cacaaaacgg
    29641 atcccggggc agcgcgtcag cgtgccgctg tagagcaggc tgagcaccgt gcgggtggtg
    29701 tcgaatggga attccatgat cgacgccggg aaatccggca tcaggtcgcg gcaggcgtcg
    29761 gtcacggtgg gatgcacgaa cacggtggcc ccgagcgcgt tcaacgcgtc gaacaccggc
    29821 gcgaaagccg ggtcgccgag atggcgattg ccgtagcagc tcagcaagcc gaagccatcg
    29881 gcatgcaaag tctccgcgac gtagcggatt tccgcgagcg tcgcatcgat gtccggcagc
    29941 ggcagcgccg cgaacatgcc gaaccgatcg ggatggcggg cgatcagcac cgccatgcag
    30001 tcgttgcagc ggcgcccgag acgggccgtc gcggccggat cgccacacca caggccgggc
    30061 gcggaaatcg acagcaacgc cgaggcgatg ccgttctcgt ccatgacctg gagcgacgat
    30121 tcgggggtcc agtccagcgc gaccgaggaa tagctcggcg cctgcttgtg catggcgtcg
    30181 gcgccgatcg cctctacata gaagtcgggc accaggtgat ggtgcacatc gatgtatgaa
    30241 gtcatttttt caaaccttgt tttatggtgc ggcgcaccca tccggccggt ctccctgccg
    30301 ggccggttca aggcggcgac agcggcgcgg acaacgcttg acagcatcgt acaagcgcgc
    30361 acaatgcgcg gaaaccagat aagcctaata agcgaaatcg acaccgtgaa tatcagacag
    30421 gtcgatctca acctcctgcc cgtgctcgat gcgctgctgc gccatcagag cgtgacgctg
    30481 gcggcacggg aattggacat gagccagtcg tcgctgagcg ccgcgctggc ccggctgcgc
    30541 accctgctcg acgacgagct gctggtgagg accgggcacg gcatgcagcc caccgcccgc
    30601 gcgacggcgc tcgccggccc gctggccagc ctgctcggac aactgcgcga cgacatcctc
    30661 acgaccgaaa gcttcgcgcc ccgcaaggtg cggcgccgtt tcagcatctg cctgtcggac
    30721 gtgggcagct acgtgctgtg gccgcagatc gtgcgcgcgg tgcgagccca ggcgccactg
    30781 atcgaactgc gcctgcgcaa tctgtccgaa caggcactcg aactcgcgat cgagcggggg
    30841 gaagcggact tgatcatcgg cgcgttcgca acgcccaagg catccctgtt ccagcggcgc
    30901 ttgtacgagc gggcctacgt ctgcgtcgcg agggcgcatc atccgtatgc gggaacccgc
    30961 atgaccttgc ggcagttcgc caatgcgcgc catatcgtgg tgcgaacctc cgcccgcgtg
    31021 cacgagcaga tcgaccagca gctgcgcgcc cggcacctgg aacgatcgca gatcacggag
    31081 ataccgtcat acctgctgtc cccgccgcta ctcgaatccg acgactatct ggcggtcata
    31141 ccaggccagt tggccgacgt attcgacgga cgctggaatc tggtcacgct ccccgtcccg
    31201 ctggcgctgc cgatgagcac catccggatg tggtggcatc gccgggccaa cggcgacccg
    31261 ggcaaccttt ggctgcgtca gctgatcgcg gcggaattgg gcgacggcaa cgatccgggg
    31321 tagacaagcc ctgccctagt cttcgcgcgc cagcggcagc ttgctgcccg agatcgcccg
    31381 ctcgagcatg acgtcgctgc gaaagcgata cagcttggcg ggccggcccg ccacgccggc
    31441 cacgatggcg cccgtttcct ccaccaggct ctgctgctcc accaggcggc ggaaattctg
    31501 cttgtgcagc ccgcggccgg ccagggcctc cacggtcgac tgcaactgcg acaaggtgaa
    31561 ttcgagcggc atcagttcga acaccaccgg ccggtacttg atcttggccc gcagccgggc
    31621 catggcggtg gccaggattc ggcggtggtc atagcgcatg gccgcgccgg gcagcaggcc
    31681 cgcctcgtgg gccgcgcgcc cctgccgggt ggcctcggcc accagcccgg cctcgaacag
    31741 cagctcgtag cgctgcagca ccatgtcctc gctccaggca tgcccgccgc tgccgaaggt
    31801 catatgcacc cgctgcatgc gctgcgcctg ggccgcggcg tttgccgcgc gccgcgccca
    31861 ggcctgcagg cgcggcagga cgagatcgtc gaccaggggc gcggcgctgc cgcgccggtc
    31921 ttcccagggg aaatagctgt accaagcctg ccacgccccc ggcacggccg cctcgacctg
    31981 gcgctcgcgc gtcaggccga gatagctgat ggaaatggtg cgaacgccgg tttcggccgc
    32041 cctgtcccgg tctgaaaagg tatagagctg ctcgacatag cccaggggat ggcgcgtttg
    32101 cgcctcgacc caggcgcgca gcccgccttg caggctcagg tggccgacct cgaaagggcc
    32161 ggccggcagc atcgtgccgc catgcaaggc cagcacccgc ggctcgccgg acgagacggc
    32221 gacgagcacg gccacgagct ccgcgctgac ggattgatcg gaagtaagag gcacgggcaa
    32281 aggtatgagt gatggcatcg aacgcgaacg acgccaccac aggccgcgtc gttaccgctt
    32341 catgcccttt gaagccaacc agccatcgat gataccggct atctgatcgg agttgcgatc
    32401 catcatcatg agatggccat tgccatggat ccccagagaa ggcaattcca gccattcggc
    32461 gttcctgtcc accttggaca aggcctcgta gaacttccgg ctgacggact ggaagcgcgg
    32521 ccagagcgga tcgtcggcaa tatggtcgcc ccacaggtag agcgtgggaa ccgacgccat
    32581 ggccgcgaca ggcaccttgg ccgggtccgg gctggacgac gcctcgacca gcaccaatgc
    32641 cttgattttg tccggcgcct tgagcgcggc ctgcacggca aaggacgtgc cctggctgtg
    32701 cgcgaccagg atgcagggac acgccttttg aacgtattcg tcgtaggcct gctgggtaat
    32761 gccgtcgttg gtcagccaac gcggcgtcga ttgcatggcc gcgacatcga aatccgaaac
    32821 ggggaattga ctgtccggat aggccgtctt gtcgccgctg ttcttatacg atcccatcgg
    32881 gccgatccgg aacagttccc agaattcctt cttggcccgg aagatcggtg ccgacgcgta
    32941 gatctgcgga aactgcgacc agcctgaacg gcctctttcc acggaatccg agatatagac
    33001 gtcataaccc gcgcccagaa agtattgctc ccatcccggc ttgccgtcag gcttggtctg
    33061 ccacgtcacg ccgctcatgc cgccgccatg ccacatcaac atgggatagc gcgctttcgg
    33121 atgggacagc cgggtgtagt tgacgtacat ctggcccgtt tcgaattctc cgttcgggtc
    33181 atagggaaag ggcgccatgc cggcggaggt gctgatggag gtcttgggca ggccggacag
    33241 cctggcctca tgcccgccga cgtagtaatt gccgattgcg gcgacatcga ggccgctgct
    33301 cgcgcagcct tgcaaaacag ctgccatgcc taccgccaaa acgccggggc gaacgatgcc
    33361 cgcccaccac gagggatgaa ccatatttct ctcctgaaac gaaggaactg ctggatcagc
    33421 gggaatcggg aggccgccgg ggcggccaga ccatctccag ctcaagggca accgaatggg
    33481 ccaggccatc caggtcgggg aacagggacg cgttggtgat gttgaggcga taaagctcgc
    33541 gcatggccga cttgcgcaat tcgatcggca gctcgatctt gcacagcagc tcttcgctgc
    33601 cgccatagcc cgccaggatt tcctcgagcg tctggtcgat cacgccgggc accacgaaca
    33661 tgccggcctg cgcgatcagg cgctggtcca tctcggccgg ttcgccgaac cagatcacgg
    33721 aatgctcgtt gctcaggaac agcttttcga aattgcccgg cgagcgcggg tcgatgaagt
    33781 cccgcgtgag ctgcggcgag gccgtcggca cggcgcggtt gagccacagg gccggcgtgt
    33841 tcagggcata gaccgacgag atgcccagcg cgcgctccag ggcgaagaag gccgccacgt
    33901 gcggcgactt ggtgaaatcg agcaggcgcg tcggcgcgcc gtgatgctgc atcatgccga
    33961 cgcagcgcag gtcgtcgtac aaggcgcccg ggtccgaaat gtagttgtgc gccttgcggc
    34021 ggaagatgcg tatggcgcgc tgctcctgca cgccccaggc gccgcggtcc tccacatatt
    34081 gggtcaggta gcgcgacagc gaactctgca ggggccagcg cgcgtcggcc tggccgcgaa
    34141 aagcccagcc gtcgagctcg gtggtgagtc cgatgaattc cgcccagctc gatacggtca
    34201 agacgcgcac gatgcattac tcctgtagcg ttacgccgct gtgccgcaag gcactgcgcg
    34261 cggccgcatc cagcgcagcc tgtgtgtcgg catggatgcg gtagagcggc tcggcggccg
    34321 ccacggcgtc gcccggccgg cgcagcaggt cgacgccggc gctcagatcg cgcggcgcgc
    34381 ccgcttcgcg cgcgatctcg gtaatgcgcc agccgtccac ctgggcgacc acgcccgccc
    34441 gcccggcgca gacatcgtac gccaggcggc ccggcaggat gggcggccgg gccctgccct
    34501 gcgcgtcgat gatgcgctcg aaggcggcca gggcggcgcc gctgtccagc agggcccgcg
    34561 cccgctgccg gcccgcctcc aggctgccca cgtccggcgc ccaggccagg atgtgcgcag
    34621 ccaccagcag ggctttctca agcaggtcgg ccggcgcgtc ggggtcgccg ctcaggacgc
    34681 ggcgcacgtc gcgcacctcg agcgccgggc cgatgcccgc gccgatggga cgcgtgccgt
    34741 cggtgaccag cgccttgacg cgcaacccca tgcctgcccc gacctcttcg aacaggcgct
    34801 gcatttcccg cgcttcttcc acggtgcgca gcttggcgcg cggaccgcag ggcaggtcga
    34861 cgacgatatg ggtggaaccc gccgaatatt tcttggacag gatggaggcg accgaccagc
    34921 ggttggactg cagcttcaac gccgccgtgc gcgcattgat gcggtcgtcg atcagcgtat
    34981 ggttgagctt gccgttccag gcgatgcagg cccgcgcctg cacgatgcag cggcgcacgt
    35041 cctgcggatc gaggtcgacc ttggccacgg tctgcatggc atcggccgtg ccggccgccg
    35101 aggtgatcgc ccgcgacgag gtcttgggca tggccaggcc gtgcgcggca acgatgggaa
    35161 cgacgatgag cgtgatgcgg ctgcccggga tgccgcccag ggaatgcttg tcgaccacga
    35221 tgggctcgtt ccaggcgatg cgcggcatgg cccgggtgcg cgccttggcc agcgccagca
    35281 cttcggcgtc ggccaggtga tcgacggcgt gcatgagaaa ggcgtctatc gtcgcatcgt
    35341 ccagcctgcc ggccacgatg tcgtcgatga cggccgcgta ctggtcttcg tcaagcgccg
    35401 cgccgctcag cttggcgtcg atcgccgcct tgctggaagc cggcgccagg gcgccgcgca
    35461 gcgccgccag gaaacgggcc acgccttctt cgagcgtgcc gtcgttcatc acgcgcagca
    35521 cgcaggccga ctcgccgacc tcggcccggc gccgcagccg ctggcccgcc tgctcggcgc
    35581 tttcgcggcc gcgcgacagg atgcgatcgc gcagcagttc gggcggcgcc gtcacttcga
    35641 tcacgaccag gcgcggcacc ttgcgggcca gctcgtcgat gacggtgcgc gagccattgg
    35701 ccagcacatg ccgcccctgg gccagatggt ccagcagttc gcggcgcagg ccgtattgca
    35761 ggccgtgcgc gccccaggtg acgagaaaag caccctggct ggccagcgcg gcgaacgcgg
    35821 cctcgtcctg cgcctcgtgg tcttcccccg gcgagccggc cggccgggta atgacgcggc
    35881 gcgcgaccag caggccgggt tcggcctggc gcgcgccgtc gatcagcgtg tccttgccga
    35941 cgccgctggg gccgaccacc aggaaaaaaa cgccggaagg catgtcgtgt cgggcctggt
    36001 tcaatcaggc ctcgtccagg ccgatgtcca cgcacaccga ggcctggttg atcttgctgg
    36061 tggagatgta gtccacgccc gtttccgcaa tggcgcggat ggtggcgagg ctgatgccgc
    36121 cggaggcctc gatcttggcg cgcccggcca ccagggccac ggccttgacc atctcgggga
    36181 tggacatatt gtcgagcatg atgacgtcga ccccggcctc taccgcttcg gccacctgct
    36241 ccacgcgatc gcactcgacc tccagcttgg tcagcgccgg cagcttgcgg cgcgcccgtt
    36301 cgactgcctt ggcaatgctg ccgcagacca cgatgtggtt gtccttgatc atgacgccgt
    36361 tgtccaggcc caggcgatgg ttcaggccgc cgccgcagct caccgcgtgc ttttccagca
    36421 tgcgcaggcc gggcgtggtc ttgcgggtgt cgagcaggcg caccttggtg ccttccagcg
    36481 tcttgacgta ctgcgcggtg agattggcga tgcccgacag gcgctgcacg atattgagcg
    36541 cggtgcgttc ggtcgtcagc acgctgcgcg ccgcgccgct gaccaccagc atcacggtgc
    36601 ccttttcgat gaactggccg tcgcgcactt tcacgtcgat cttcaggctg tcgtcatagc
    36661 gctggaagat ccgggtcgcg acgtcgatgc ccgccacgat caggggctcg cgcgcattca
    36721 tctggaactt gccctgttcg tcgggctcga tcatggtctg cgccgtcagg tcgcaatagc
    36781 cgatgtcttc ggccagccat tggtcgatca gtcggtcggt caggaaggga tcgtacatgc
    36841 tgcactcctc gggataattc gattcagcgt ttcaaggatg gaaagggatc aatggtgcga
    36901 cagcctgcgg ccgagccgca tcagcaggcc gttgcagacc agggcgaaca ggacgagcag
    36961 caaggccacg gccatgatgg tcttgatgtc gcccaggttc atggcgttgg tgagcaggta
    37021 gcccaggccg cgctgcgagg cgaacatctc gccgatgagc acgcccagga gcgtgagcga
    37081 aaaacccagc cgcaggcccg ccaccacctc cggcaggatg gccggcagca cgatgtgcca
    37141 catggcccgc atcggcgcca ggcgcagggt ggccgacacg cgcaaaaaga ccggccgcat
    37201 ctgcgcgatg gcgctcatgg tgaacaccgt gatggggatc agcccgtgca tcacgccgaa
    37261 ggccaccttg gcgggcaggc ccaggccgaa caccagcagc accagcgggt agagcgtgac
    37321 cttgggcagg gaatacaggt tgagcaggat gggctcggcc acttcgcgcg ccaggcgatg
    37381 cacgcccagc gccatgccga gcgccaggcc gccggccacg ccgatgacga gcgccagcac
    37441 cagggcccgc agcgtttcga acagatgcgg ccagaaggcg gccgagccca gcaggctggc
    37501 cagttcgcgc accgtgtccc acggcgaggt cagcgcgcca ttgcccgccg ccaggtgcag
    37561 gatctgccag gcggccagca ggatcgccgc catcgccagg gtattggaaa ctgtcttcat
    37621 tccctttccc ctagcggcgg cggcgcgcct gcaggcggcg gtcgacggca ttgagcgcca
    37681 tgttgatcag cgtgacgagc accagcacca ggaacatcag cgcgtacatg tcgcggtttt
    37741 cgaagttgtt gtaggcgtag ccgatggcat agccgatgcc cgagcccgag agaatgaatt
    37801 cggaagcgat gacgccgatg aaggcatagg ccaccgacag cttcacgccg gtgaacaggt
    37861 agggcaaggc ggccggcagc ttgacgcgca tggccgactg ccagggcgac aggcgcagcg
    37921 ccagcgccgt cttgtgcagg gcgcggggga tgcggtccag gccggtgagc gtggccgtca
    37981 ccatggacac caccgccagc aggacggcaa tggccacgat gggcgccgcc cccacgccca
    38041 ggaggacgat gaagacggga tagaacacga aggtgggcac ggcgtaatag gccgacagca
    38101 gcggctcgac cgccgcccgc acgcgcggcg cgctgtgcag caccacgccc acggcaaagc
    38161 ccagcagcac cgagaggatc gcggccgaga cgatattgcc caggctgatg gcgatgtcgt
    38221 ggtcgaactt gcccgcgcgc agcagctcga cgcagtgcgc cgccatgtcc gacggcgcga
    38281 tcatgacgtt caagggaatg atgccgatgc ggcacagcag ttcgacggcg gcgatgaaca
    38341 gcaccaccac ggccagccgc acgacgctgg cgcgcgacag gccgcgatgg cggatgacgc
    38401 ccgcggcgcc aaccgatgcg gtatcagtgg ccatcggcga tacccatgac cttgagcgat
    38461 tcgacgcgca gtttttccca caggcgcgcg ttgacctcgc cgaaacgcgg cgtggacacg
    38521 atgcggctgt cgcggtcgcg ctcccagccg gtttcgacga tgtcgatgaa gaggccggga
    38581 cgcgaagaca tcacgcccac ccggtcggac agcatggccg cctcgtccag cgcatgggtg
    38641 atgagcagca cggtggccga ggtttcgcgc cacagccgca gcagctcatc gcccatgagc
    38701 aggcgcgtct gctggtcgag cgcgccgaag ggctcgtcga gcaggatcag cgaaggtcgg
    38761 agcaccagcg ttcgcgcgat gcacacccgc tgtcgcatgc cgccagaaag ttgcgccgga
    38821 taggccgctg cgaagtcctt cagggacatc aggtcgagcg ccgcgtcgat tcgcgaatcg
    38881 gcctcgtccc gaggcacgcg cttgcgcttc aggccgaaac cgatgttttc tcgcaccgtc
    38941 agccagggaa agctcgaatc ttcctggaac acgacgccga catcgtccgg t
//
LOCUS       DASWPO010000149         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1104807, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000149 DASWPO010000000
VERSION     DASWPO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104807"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1277)
                     /locus_tag="VGC69_08275"
     CDS             complement(102..1277)
                     /locus_tag="VGC69_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295311.1"
                     /translation="MLTDFFYHLREHRLPVSVQEYLTLLDALRADGLMAPTLDDFYFL
                     ARTALVKDETLYDRYDQAFGAYYRGIAAKLPPAKDIPLDWLIKEFEKSLTPEEKAAIE
                     KHGWDKLMELFKQRLEEQKDRHAGGSKWIGTGGTSPFGNGGYHPEGIRVGGDSAGNRT
                     AVKVWDMRQFRDYDDQVELGTRNFKVALRRLRRFAREGGELELDLDDTIASTARNAGW
                     LDLRLVPERHNNVKVLMLLDVGGSMDDHITRVEELFSAARGEFRNLDVYYFHNCPYDS
                     LWQSNRRRREERFDTWDILRRYNEDWRLIIVGDATMSPYEILQPGGSVEHHNKEAGAV
                     WMRRLLDAWPKSVWLNPEPSNYWQYRQSIALMRDIMQERMYPVTVAGLEQAMRWLSK"
     gene            complement(1303..>2068)
                     /locus_tag="VGC69_08280"
     CDS             complement(1303..>2068)
                     /locus_tag="VGC69_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0295312.1"
                     /translation="PLLIKGEPGTGKTMLAEEVARALDRPLLQWHIKSTTKAQQGLYE
                     YDAVSRLRDSQLGDEKVRDIRHYIVQGTLWRAFDAERPVVVLIDEIDKADIEFPNDLL
                     RELDRMEFHVYETQQTVAARHRPLVIITSNNEKDLPDAFLRRCFFHYIRFPDRETMRD
                     IVAVHYPELKADVLRAALDTFFALREAPGLKKKPSTSELLDWLRLLLAENASAEQIDA
                     HAAAAVPMLAGALLKNEQDMQLLDRLAAMTRGAQRR"
BASE COUNT          315 a          673 c          702 g          378 t
ORIGIN      
        1 cagcccgccc ctaggccccc cagcgactat gcgcctcaag aactcaaatc aaccaaccaa
       61 acgcaacagc aacgcaacag cagcaacacc cgcagcctca gctatttcga caaccaccgc
      121 atcgcctgct ccagccccgc caccgtcacc ggatacatgc gctcctgcat gatgtcgcgc
      181 atcagcgcga tcgactgccg gtactgccag tagttcgacg gctcgggatt gagccacacc
      241 gacttgggcc aggcgtccag cagccggcgc atccacaccg cgccggcttc cttgttgtgg
      301 tgctcgacgc tgccgccggg ctgcaggatc tcgtaggggc tcatggtggc atcgcccacg
      361 atgatgagcc gccagtcctc gttgtagcgg cgcaggatgt cccaggtgtc gaagcgttcc
      421 tcgcgccggc ggcggttgct ctgccacagg ctgtcgtagg ggcagttgtg gaagtaatac
      481 acgtccaggt tgcggaactc gccgcgcgcc gctgaaaaca gctcttccac ccgggtgatg
      541 tggtcgtcca tgctgccgcc cacgtccagc agcatcagca ccttgacgtt gttgtgccgc
      601 tcgggcacca ggcgcaggtc cagccagccg gcattgcggg ccgtgctggc gatggtgtcg
      661 tccaggtcca gttccagctc gccgccctcg cgggcaaagc gccgcagccg gcgcagcgcc
      721 accttgaaat tgcgcgtgcc cagttcgacc tggtcgtcgt agtcgcggaa ctggcgcatg
      781 tcccacacct tgaccgccgt gcggttgccg gccgagtcgc cgcccacgcg gatgccttcc
      841 gggtggtagc cgccgttgcc gaagggcgag gtgccgcccg tgcctatcca cttgctgccg
      901 ccggcatggc ggtctttctg ctcttcgagg cgttgcttga agagctccat gagcttgtcc
      961 cagccgtgct tctcgatggc ggctttttcc tcgggcgtga ggcttttctc gaactccttg
     1021 atgagccagt ccagcgggat gtccttggcc ggcggcagct tggccgcgat gccccggtag
     1081 taggcgccga aggcctggtc gtagcggtcg tacagggttt cgtccttgac cagggcggtg
     1141 cgggcgagga aatagaaatc gtccagcgtg ggcgccatga gcccgtcggc gcgcaaggcg
     1201 tccagcagcg tcaggtactc ctgcaccgac accggcaggc ggtgttcgcg caggtggtag
     1261 aaaaagtcgg tcagcatggc gggcgggcgc aggccgggcg tgtcagcgcc gctgggcgcc
     1321 gcgcgtcatg gcggccaggc ggtcgagcaa ttgcatgtcc tgctcgttct tgagcagggc
     1381 gccggccagc atgggcacgg cggcggccgc gtgggcgtcg atctgctccg cgctggcgtt
     1441 ctcggccagc agcaggcgca gccagtccag cagctccgag gtcgagggtt ttttcttcag
     1501 gccgggcgcc tcgcgcagcg cgaagaaggt gtcgagcgcc gcgcgcagca cgtccgcctt
     1561 gagttcgggg tagtgcaccg cgacgatgtc gcgcatggtc tcgcggtcag gaaagcggat
     1621 gtagtggaag aagcagcgac gcaggaaggc gtcgggcagg tccttctcgt tgttggaggt
     1681 gatgatgacc agcgggcggt ggcgcgcggc gaccgtctgc tgcgtttcgt acacatggaa
     1741 ctccatgcgg tcgagttcgc gcagcaggtc gttggggaat tcgatgtcgg ccttgtcgat
     1801 ttcgtcgatc agcaccacca ccggccgttc ggcgtcgaag gcccgccaca gcgtgccctg
     1861 cacgatgtag tggcggatgt cgcgcacctt ctcgtcgccc agctgggaat cgcgcaggcg
     1921 cgagacggcg tcgtattcgt acaggccctg ctgcgccttg gtggtggact tgatgtgcca
     1981 ctgcaagagc ggccggtcca gggcgcgggc cacttcttcg gccagcatgg tcttgccggt
     2041 gccgggctcg cccttgatca gcagcggg
//
LOCUS       DASWPO010000150        13854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_925152, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000150 DASWPO010000000
VERSION     DASWPO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13854
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925152"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..996)
                     /locus_tag="VGC69_08285"
     CDS             complement(145..996)
                     /locus_tag="VGC69_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295313.1"
                     /translation="MKRNSKIRPLVSPVSRMVACATLLLGSMLVHAQTLDEAKKALPD
                     AIKNSGTIRVATSLTWAPFAYLDNGQATGIDIELITLLAQKLGLKTTFDDLKFPAIVP
                     GLSTGRYDVGVDQMGITEERKKAVDLIPYFRSGYGLLVKKGSAPLNINKLCGHTLALT
                     QGSSQIQLVDEMSKACETSNQKAITQLFYPDAPSTMMAVATGRGEGFITDRAVSIYIA
                     KHHQDLDAMPEMLDGKTLVAGIAVGKQLPTLKQALQVALQAAVQDGSYKALLTKYGVP
                     DSAYTEE"
     gene            1433..2224
                     /locus_tag="VGC69_08290"
     CDS             1433..2224
                     /locus_tag="VGC69_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0295314.1"
                     /translation="MSLSLSFENAVVLVTGASKGIGLACARAFAQAGARVAGVSRSAA
                     NLDAARAELQAAGLDMRTYAVDLIDPLAALDMVEQVERDLGPITVLVNSAGAARRYAP
                     EQLNAQALQQGMDAKYFSYMNVLDPVAKHMGQRKRGSIVNIIGQGGKVASPFHIAGGS
                     ANAALMLSTAGYARAYAAQGVRVNAVNPGLTQTSRVDEGLAVTAQSSGRSRDDVLAEQ
                     LAGIPMGRMALPEEVANVALFLASDLASYVSGAIIPMDGCVSSVI"
     gene            2282..3169
                     /locus_tag="VGC69_08295"
     CDS             2282..3169
                     /locus_tag="VGC69_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0295315.1"
                     /translation="MSTSSRFPFKRLLAAGLLVVLGVVGCSRLDDWQRQLIFDPQTDA
                     RPWWREPPAGTEIYDLPLAGGDTVRAWFWQSPRADAPTVLYLHGARWDLNGSAFRMEA
                     WTRMGYSVLAIDYRGFGASTARLPSERSAMQDAVAALHELARRQPDPARRFLYGHSLG
                     GAIAIDLASQPGMPAVAGLVVESSFTSMADMMGTLKWGWLPGADLLITQPFDSIDKVA
                     GLTLPVLFLHGTDDRVVPPAMSDALYAAAREVPANLKRLVKIEGASHSSAVRSGKPYE
                     TAVRTFIHDAGQAYGVAHG"
     gene            3231..3692
                     /locus_tag="VGC69_08300"
     CDS             3231..3692
                     /locus_tag="VGC69_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0295316.1"
                     /translation="MKPESADPASATAAALAAELRIVIGALNRRLREQADASDLTSAQ
                     KSVLLRLERDGPATGSALARAEAMRPQSMGAIITALETAGYVAGAPDPADGRQTIISL
                     TDHFRDWVRAARAARQDWLIRTLQARLTAQEQHQVAGALELLKRLLGAGQP"
     gene            3702..5126
                     /locus_tag="VGC69_08305"
     CDS             3702..5126
                     /locus_tag="VGC69_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0295317.1"
                     /translation="MTDQADRLDPNVWKVVCVAATGSFMAQLDATVVNVSLASLAADL
                     RVSLAAIQWVTSGYLLALALMLPLNGWLVDRIGAKALYLWCFAAFTLSSTLCGLAWSA
                     DSLIVFRILQGMSGGLLAPMAQMMIARAAGRQMARVVSIAALPVLLAPLLGPVIAGAI
                     LQFASWRWLFLVNLPVGVLALALAAAFLPNDREETRPRGLDLLGLALLSPGLVLFLYG
                     SDHLGERIGGAALAVSLIMFALFYRTARAKGDAALIDLRLLKSKAFSASIAAMFMVNG
                     ITFAGQMLIPIYLVHACGLSPSQTGWLLAPLGLGMMCTYPFLGRLTQRFGIRKLAACG
                     ALLALAGTLPLVFLAQHGLVVAVLAASLFIRGVGVSAIGVPSITSGYASVARQDLPMA
                     TTAMNIVQRLGGPTLTTLCATFLGWRMSATPDTAAASGPFVAAFGLLCVLHALLFLAT
                     LRLPWLVPQSGPGPHSSATDPRPA"
     gene            complement(5079..5714)
                     /locus_tag="VGC69_08310"
     CDS             complement(5079..5714)
                     /locus_tag="VGC69_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0295318.1"
                     /translation="MARAVTERADVIAPLAATFRTHGFAGASLSVISKSTGLGKGSLY
                     HFFPGGKEEMAQAVLDQVSAWFQLNVNEPLAAAGAPGARIAAMFTACEAYFRSNQLVC
                     LFGAFALGQEQRRFTGQIRRYFDDWTIALAAALRDAGWPPAQASARALDVVAGVQGAL
                     VIARASDDPDVFAALLGRLQRGTQTALVASDAISRSSGRPRIRRGRVGPGS"
     gene            complement(5714..6181)
                     /locus_tag="VGC69_08315"
     CDS             complement(5714..6181)
                     /locus_tag="VGC69_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0295319.1"
                     /translation="MARPPLPPFTRESAIQKVRAAEDAWNTRDPERVALAYTEDSRWR
                     NRSTFVTGRGQIVEFLAAKWQREQEYRLIKELWAFDGARIAVRFAYEWRNAEGQWFRS
                     YGNENWFFDEDGLMAERHASINDLPIDASERLYFWDQSVARPADHASLTELGL"
     gene            6370..8190
                     /locus_tag="VGC69_08320"
     CDS             6370..8190
                     /locus_tag="VGC69_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0295320.1"
                     /translation="MPQPLQRLIALRHVASQISADEDIDALLRDLLRSACQQGGWDMG
                     AIMSIDLANGYGTVVTRHEATILHRLMEERWDLATSPSLIALQKGEPIYIRNAQESEQ
                     FPGYRREAKLRGYRTVLVCPVASSDTEGRPLVLSVSSRQIRDVDEELMAFMTTIVHLG
                     AIAIERAHRNRAKTRHTEQLQRVLGAQRTLLRDVLEGEPADALATTLDELLNGPVLIA
                     DFFGGSLVGSRSPVPGIYGDAAWRGLLAGPLGREVFYTIRDAVKLGRKSSIALPLGHE
                     ATIEAEVESLTVDAETVGALLTFGQAPNGDLHALMMESARFALSVHFMRSVIQFRYET
                     RTLTELFFEIVERRWRDEQDVLYRGHHLGLALAEPLRMLVIDYPQTAGRGDNPSVECQ
                     NIVTLLARQQGIALHVVTVGGGLVCLIPQAGNADPDGLMRLARRMAEALRRPLGCEPI
                     VVLSDTCVGLERLAMEWERCWRMIRIARDFGKSGVLGMPDLGPLPMLVGAADSADVRD
                     FIHGAIGKLVECDQQTHSPYLETLAAYLRSGCRGQPCADAMGLHVTTLRYRLSRIKSL
                     FGIDLDSPERRFAIELAINLHKLTRGESPATGSPESLHRT"
     gene            8340..9638
                     /locus_tag="VGC69_08325"
     CDS             8340..9638
                     /locus_tag="VGC69_08325"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HEY0295321.1"
                     /translation="MHLVAYLKTGPTARHAGGWRHPEAVLDDFLSPSRYIHLAKLLES
                     AKFDGCFFADLFGLYDIHDGNFDAYVRRGGQISYLDPMTVLPIMAAATSKLGLGATLS
                     TTFYNPYHLARALASLDVLSGGRAAWNVVTSATELEAQNAGMDQLPSKDERYDRADEV
                     LEACMALWNSWDADPFLLDKAQGTFADPSKVHYANYKGKWVKTRGPLSIPRSPQGRPV
                     IMQAGGSDRGRIFAARWAEAIFTVQRDQEGMRRFYQEIKGGLAKWGRSENDCAILPAV
                     SVVVGETESIAQERAEYLRSLVDGELVAASASSNLGVDINSVASEKELASKQRNQGMA
                     GSEILIKQSMAANNIGFKEAAQKYQPDMLVGTPQSIADQLQHLFESEVCDGFIVTPTY
                     SPGMLEQFCRAVVPELQRRGVFRKEYSGSTLRQHLQSDAA"
     gene            9659..10729
                     /locus_tag="VGC69_08330"
     CDS             9659..10729
                     /locus_tag="VGC69_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0295322.1"
                     /translation="MTDRLKIEHLYKVFSDAPGPALALLRSGRSKTEVQEQLGQVVGL
                     DDISLDIPSGAMYMIMGLSGSGKSTLARCINRLNEPSAGRILLDGQDICALDETGLRE
                     VRRNRISMVFQHFALLPNRRVIENVEFGLKLRGVSAKERRRHAQEVLEVVGLARWAYH
                     MPQQLSGGMRQRVGLARALATDADVLIMDEAFSALDPLIRSEMQDELLRLQRTLKKTI
                     LFITHDFHEALKLGTRIAIMAEGRLVREGTPQSIVLEPGSDYVAAFTRDVDRARLFDA
                     ASAMTPLSDWRGDGSAATGVPDGLVCVPGTAKLFEVAGKYASAGPLLGVTDDAGRLIG
                     VLEAPQVLARIGQAQAAEAHHA"
     gene            10722..11669
                     /locus_tag="VGC69_08335"
     CDS             10722..11669
                     /locus_tag="VGC69_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0295323.1"
                     /translation="MLDADQLAFLPVDLWIQGGVKWLASNLRPLFLTIKWPIEQLLAM
                     CDTALHAVPFVVLVLVAALVVWRLAGAAVAAFSAVVLVVIAMLGVWNEAMTTLSLIVT
                     AIVFCAVIGIPIGIACARSDRVWMIVRPILDVMQTTPTFVYLVPVVMLFGVGTVPGEV
                     AVVTAAMPPLIRFTQLGIRMVDHEIVEAGLAFGADRRQLLWEVQLPLAVPTILGGLNQ
                     TVLTAMVMSVVVAMIGAEGLGLVVLQGLGRLDVGRAAVGGIAIVLLAIMLDRITQKLP
                     TFEPFARLGLLSVIARFLRGGRPAADSAAKRAEDIKGVL"
     gene            11722..12747
                     /gene="proX"
                     /locus_tag="VGC69_08340"
     CDS             11722..12747
                     /gene="proX"
                     /locus_tag="VGC69_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HEY0295324.1"
                     /translation="MKTYVKGYAAALLAVLAINLPAAAQDLPGKGKTVRYAQGDSLGS
                     NYVVSLIVSQALKDMGYGVKLSTMNTTLFFQAAAQGDQDIATDINFPQRELAFKAVAD
                     QETIIGTGLIIGGGVNGYMIDKKTALAHNITSVDQLKDPKLAGLFGADGKAELISCDP
                     GWSCGDVVDYQLDKFGLKQTVKSVRGKYEALMVDTIAKVNSDKPVLFYTWSPSWVTST
                     LVPGKDVVWLPTPMDALPPNITARGSALVPNVEGCAGGANPCRMAMASWNWGVVANNA
                     FIAANPAVKALLEEIKFPLSTFSHWEGEIGKNGGSQATINRLSSEWLGANKTQFDQWV
                     ATAAKVH"
     gene            complement(12809..13528)
                     /locus_tag="VGC69_08345"
     CDS             complement(12809..13528)
                     /locus_tag="VGC69_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0295325.1"
                     /translation="MENKLDDLVTPEKRSLDRAAADSLRQAIVTGVFPPGARLTEIRL
                     AERLSLSRGTVRAALHRLVTEGLVVQQPYTGWHVMKLTPHDAWEISTLRARFEGLGVR
                     LTAENIDDERRKALRAGLDALGRAVRQGDHSALTSADLGLHKLIVDLSGHRRLSQHYA
                     PVTHQIRLYIAATTAMQPSYDLVFEAHCAIVEAICAGEADKAEAMASEHCLGSGRRLV
                     ARLEEEEGAARLPRGAADAVL"
BASE COUNT         2344 a         4630 c         4398 g         2482 t
ORIGIN      
        1 cgatctccca ctggaagccc gggttcgtcc cgatctggta gaccacccag gccacgaaga
       61 ccagggccag gacgatgcag accccccgcc cgggccgggc gcgctcggcg atcttgattt
      121 cgacgtctgt catgccgggc ctgcttactc ttcggtgtag gcgctgtcgg gcacgccgta
      181 tttcgtcagc agcgccttgt agctgccgtc ctgcaccgct gcctgcagcg ccacttgcag
      241 ggcctgcttc agggtgggca gctgcttgcc cacggcgatg cccgccacca gcgtcttgcc
      301 gtccaacatt tcaggcatgg catccaggtc ttggtgatgc ttggcgatat agatggaaac
      361 ggcgcggtcg gtaatgaacc cttcgccacg gcccgtggcg accgccatca tcgtgctggg
      421 cgcgtcaggg tagaagagct gcgtgattgc tttctgattg ctggtctcgc aagccttcga
      481 catttcgtcg acgagctgga tctgggagct gccctgcgtc agcgcgagcg tatggccgca
      541 cagcttgttg atattcagcg gggcgctgcc cttcttgacc agcaggccgt agcccgagcg
      601 gaaataaggg atgaggtcga ccgccttctt gcgctcttcg gtgatgccca tctggtcgac
      661 gccgacgtca tagcgccctg tcgacaagcc ggggacgatc gccgggaact tgaggtcgtc
      721 gaaggtcgtc ttcaggccca gcttctgggc cagcagcgtg atcaactcga tatcgatgcc
      781 ggttgcctgg ccgttgtcga gataagcaaa gggagcccac gtcaacgacg tggcgacgcg
      841 tatggtccca ctgttcttga tggcgtccgg caaggctttc ttggcctcgt ccagcgtctg
      901 cgcatgaacc agcatggaac ccagcaggag ggttgcgcac gccaccatcc ggctgacggg
      961 cgacaccagc gggcggatct tcgaattccg tttcattctt tccccttcct tatctctgct
     1021 tatgcacatg gcgagacgca tgatctggcc tcgcagaagt agaatgtaga cattataaat
     1081 ttcaatgtaa aggcgattta agccaagacc agtgaaatcc ctgatgccag ggtcctcgca
     1141 aagatcacgt accaattctc tggtatgcac ttgcaggctt tcttgagaaa ttgcttgttt
     1201 tttcaattac aagtatctga tagccggaaa cgatccggac tagagagaga cgcaggcccc
     1261 tgccgtgcct tctctctggg aaagcaaaac gtatacttta ttctttattt ttataggcat
     1321 tgcgcgccag tgcgggcgcg gatgccccgc cggcaggaac ttgatggtgt cccggtagac
     1381 gcttatcgta ctgtcaaccg aacataccgc tcccctaccc ccaggaaccg ccatgagcct
     1441 atcgctgtct tttgaaaacg ccgtcgtgct ggtgacgggt gccagcaaag gcatcggcct
     1501 ggcatgcgcc cgggcgtttg cccaggcagg cgcccgcgtg gcgggggtct cgcgcagcgc
     1561 agccaatctc gatgccgcgc gtgccgaact gcaagcagcc ggcctggaca tgcgcaccta
     1621 cgccgtcgat ctgatcgacc cgctggccgc gcttgacatg gtggaacagg tggaacgcga
     1681 cctcggcccg atcaccgtgc tggtcaactc ggccggcgcc gcgcgccgct acgcgccgga
     1741 acagttgaac gcgcaagccc tgcaacaggg catggacgcc aagtacttca gctatatgaa
     1801 cgtactcgac cccgtcgcca agcacatggg ccagcgcaag cgcggcagca tcgtcaacat
     1861 aataggccag ggcggcaagg tcgccagccc cttccacatt gcgggaggat ccgccaacgc
     1921 cgcgctgatg ctcagcacgg cgggctatgc gcgggcctat gcagcgcagg gcgtgcgcgt
     1981 caacgccgtc aaccccgggc tgacgcagac cagccgggtg gacgaaggcc tggcggtcac
     2041 cgcgcaatcg agcggccgct cgcgcgacga cgtactggcc gagcagctgg ccggcatccc
     2101 catgggccgc atggccctgc ccgaggaagt cgccaatgtg gcgctgttcc tggcttcgga
     2161 cctggccagc tatgtgagcg gcgccatcat tccgatggac ggatgcgtgt cctcagtcat
     2221 ctagcggccg acaacaccgc ggaatcggcc gggcacctca gacccgccag cgctcgcccc
     2281 catgtccacc tcctcccggt ttcccttcaa acgcctgctg gcggccggcc tgcttgtcgt
     2341 gctcggcgtc gtgggttgct cgcggctcga cgactggcag cgccagctca tcttcgatcc
     2401 gcaaaccgac gcgcggccct ggtggcgcga gccgccggcc ggcaccgaga tctacgacct
     2461 gccgctggcc ggcggcgaca cggtgcgggc ctggttctgg cagtcgccgc gggccgacgc
     2521 gcccaccgtg ctctacctgc atggcgcgcg ctgggacctc aacggcagcg ctttccgcat
     2581 ggaggcctgg acgcgcatgg gctattcggt gctggcgatc gattaccgcg gctttggcgc
     2641 gtcgaccgcg cggctgcctt ccgagcggag cgccatgcag gacgccgtcg cggccttgca
     2701 cgagctggcg cgccgccagc ccgatccggc ccggcgcttt ctctacggcc acagcctggg
     2761 cggcgccatc gccatcgacc tggcctcgca gcccggcatg ccggctgtcg ccggcctggt
     2821 cgtcgaatcc agcttcacca gcatggccga tatgatgggc acgctcaagt ggggctggct
     2881 gccgggcgcc gacctgctga tcacgcagcc gttcgattcc atcgacaagg tggcggggct
     2941 gacgctgccc gtgctgtttt tgcacggcac cgatgaccgc gtggtgccgc ccgccatgag
     3001 cgatgccttg tatgccgcgg cgcgcgaggt tccggcgaac ctgaagcgcc tggtcaagat
     3061 cgagggcgcc tcgcactcga gcgcggtgcg cagcggcaag ccctacgaaa ccgccgtgcg
     3121 cactttcatc cacgacgccg gccaggctta tggggtcgcg cacgggtagc cgcccgcatg
     3181 atatgtagga aatctttgta gttaaacttc atatatgcca tactgcatgc atgaagccag
     3241 aatcagccga tcccgcaagc gccacggccg ccgccctggc cgcagaactg cgcattgtga
     3301 tcggcgcgct caaccggcgc ctgcgtgagc aggccgacgc aagcgacctg acttcggccc
     3361 aaaagtccgt gctcctgcgg ctggagcgcg acgggcccgc gaccgggagc gcgctggcgc
     3421 gggccgaggc catgcggccg caatcgatgg gcgcgatcat cacggccctg gagacggccg
     3481 ggtatgtggc gggcgcgccc gaccccgcgg acggacgcca gaccatcatc tcgctcaccg
     3541 accatttccg cgactgggtg cgcgcggcgc gagcggctcg gcaggactgg ctgattcgca
     3601 ccttgcaggc gcggctgacc gcccaggaac agcaccaggt cgccggcgca ctggagctcc
     3661 tcaaacgcct cctcggcgct gggcaaccct aaggctcacc catgaccgat caagccgatc
     3721 gtcttgatcc caatgtctgg aaagtagtct gtgtcgcggc cactggctcc ttcatggcgc
     3781 agctcgacgc caccgtcgtc aacgtgtctc ttgcaagcct tgcggccgat ctgcgcgtca
     3841 gtcttgcggc gatccagtgg gtgacgagcg gctatttgct ggcgctggcg ctgatgctcc
     3901 cgctgaacgg ctggctcgtc gaccgcatcg gcgccaaggc gctctatctc tggtgcttcg
     3961 cggccttcac cctctcttcg accctgtgcg gcctggcatg gtccgccgat tcgctgattg
     4021 tgttccgcat cctgcagggc atgagcggcg gactgctcgc gcccatggcg cagatgatga
     4081 tcgcccgggc ggcgggcagg cagatggcgc gggtagtcag catcgccgcc ctgccggtcc
     4141 tgctcgcgcc cctgctgggg cccgtcatcg ccggcgccat cctccagttc gcttcctggc
     4201 gctggctgtt cctcgtcaac ctgccggtcg gcgtcctggc gcttgccctg gcggcggcat
     4261 tcctgcccaa tgaccgggag gaaacgcggc cgcgcggcct cgacctgctc ggactggccc
     4321 tgctatcgcc ggggctggtg ctgtttcttt atggttcgga ccatctcggc gagcggatcg
     4381 gcggcgcggc cctggccgtc tccctcatca tgttcgcgct gttctaccgc acggcgcggg
     4441 ccaagggcga tgccgcattg atcgaccttc ggctgctcaa gagcaaggcg ttctccgctt
     4501 cgatcgccgc catgttcatg gtcaacggca tcacctttgc cggccagatg ctgattccga
     4561 tctatctggt ccacgcctgc ggcctgtcgc cgagccagac cggctggctg ctggcgccgc
     4621 tggggctcgg catgatgtgc acctatccat tcctgggccg cctcacgcag cggttcggca
     4681 tccgcaagct tgcagcctgc ggtgcgctgc tggccctggc cgggaccctg ccgctcgtct
     4741 tcctggccca gcacggcctc gtcgtcgccg tcctggcggc gagcctcttc attcggggcg
     4801 ttggcgtcag cgccatcggc gttccgtcga tcacctcggg ctatgcctca gtggcgcgcc
     4861 aggacctgcc gatggcgacg accgccatga acatcgtcca gcgcctgggc ggcccgacgt
     4921 tgacgacact ctgcgcgacc ttccttggat ggcgcatgtc ggccacgccc gataccgcgg
     4981 cggcgtccgg ccccttcgtc gcggcgttcg gcctgctctg cgtcctgcac gcgctgctgt
     5041 tcctggccac cctccggctg ccctggctgg tgcctcaatc aggacccggg ccccactctt
     5101 ccgcgacgga tcctcggcct gcctgaagac cgcgatatcg cgtccgaggc cacaagtgcg
     5161 gtttgtgtgc cgcgttgcag tcgtccaagc agcgccgcaa aaacgtcggg gtcgtcggag
     5221 gctcgggcga tcaccagggc gccttggacg ccggcgacca cgtccagcgc gcgggcggat
     5281 gcctgcgccg ggggccatcc agcgtcgcgt agcgcggcgg ccaacgcgat ggtccagtcg
     5341 tcgaaatagc ggcggatctg cccggtgaag cgccgctgtt cctgcccgag ggcgaaggcg
     5401 ccgaacaggc acacgagctg gttggagcgg aagtacgcct cgcacgcggt gaacatcgcc
     5461 gcgatccggg cccctggggc gcccgcagcg gcgagcggtt cgttgacatt gagctggaac
     5521 cacgccgaca cctgatcgag gacggcctgc gccatttcct ccttgccgcc cgggaaaaag
     5581 tgatacaggc tgcctttgcc gagccccgtg ctttttgaga tgaccgacag gcttgccccg
     5641 gcgaagccat gggtgcgaaa cgtcgcggcc agcggtgcga tcacgtccgc gcgttcagtg
     5701 accgcgcgcg ccatcagagt cccagctcgg tcaggctggc gtgatcggcg gggcgcgcaa
     5761 cggactgatc ccagaagtac agccgctcgg acgcgtcgat cgggaggtcg ttgatgctcg
     5821 cgtgacgctc ggccatgagc ccatcttcgt cgaagaacca gttctcgttc ccgtaggacc
     5881 ggaaccactg gccttccgcg ttgcgccact cgtaggcgaa ccgcaccgcg atgcgcgccc
     5941 catcgaaagc ccacagctcc ttgatgaggc ggtattcctg ctcccgctgc cacttcgcgg
     6001 ccagaaattc cacgatctgc ccacgcccgg tcacgaacgt cgagcggttg cgccaccggc
     6061 tgtcctcggt gtaggccagc gccacccgct ctggatctcg ggtattccag gcatcctcgg
     6121 ccgcgcggac cttctggata gcggactcgc gggtgaacgg gggaagaggc ggacgggcca
     6181 tgaggcgatc tccttggttg atatttaccg aacagtacaa ttatgtactg atcagtaaac
     6241 tatggcaagg cgtggtcttt atatcctgga ggcacgacag ggccatatct tccatcgctt
     6301 cgatggaaga attttctctt ttttgctgtg ggttcttgta aattgacagg aaacacaggg
     6361 gaaatatcca tgccgcagcc tttgcaaagg ctaatcgcgc tgcgccatgt ggctagccag
     6421 atcagcgcgg acgaagacat cgacgcgctg ctgcgcgacc tcctccgttc ggcctgccag
     6481 caaggcggct gggacatggg ggccatcatg agcatcgatc tggccaacgg ctatggaacc
     6541 gtcgtgaccc gccacgaggc caccatactg catcggctga tggaagagcg atgggatctc
     6601 gccaccagtc cttcgctgat tgcgctccag aaaggcgagc cgatctacat ccggaacgca
     6661 caggagagcg agcagttccc gggctatcgc cgcgaagcca agctgcgcgg ctaccgcacc
     6721 gtgctggtat gccccgtcgc gagcagcgac accgaggggc ggccgctcgt cttgtcggtc
     6781 tcgtcgcgac agatccgcga cgtcgacgag gagctgatgg cattcatgac gaccatcgtc
     6841 cacctgggcg ccatcgccat cgagcgcgcg catcgcaacc gcgccaagac ccggcatacc
     6901 gagcagttgc agcgggttct cggcgcgcag cgcacgctgc tgcgcgacgt gctggaaggc
     6961 gaacccgccg acgccctggc gacgacgctg gacgaactgc tcaacggccc ggtgctgatt
     7021 gccgacttct ttggcggcag cctggtcggt tcccgctctc ctgttcccgg gatctacggc
     7081 gatgccgcct ggagaggact gctggcaggc cctctgggcc gggaagtgtt ctacaccatc
     7141 cgggacgccg taaagctcgg cagaaagtcg tccatcgcat tgccgctggg ccatgaggca
     7201 actatcgaag ccgaagtcga gtcgctcacc gtggatgccg agaccgtcgg cgcattgctg
     7261 accttcggcc aggcgccgaa cggcgacctg cacgcgctca tgatggagag cgcccggttc
     7321 gcgctgagcg tgcacttcat gcgcagcgtc atccagttcc gctacgagac gcgcacgctg
     7381 accgagctct tcttcgagat cgtggagcgc cgctggcgcg acgagcagga tgtgctgtat
     7441 cgggggcacc acctgggcct ggcgcttgcc gagccgctac gcatgctggt gatcgactat
     7501 ccgcaaacag ccggccgcgg cgacaatccc tcggtcgaat gccagaacat cgtcacgctg
     7561 ctggcccggc agcaaggcat cgccttgcat gtggtcaccg tgggcggcgg cttggtctgc
     7621 ctgattccgc aggccggcaa tgccgacccg gacggcctga tgcgacttgc ccgccgcatg
     7681 gccgaggcgc tgcggcgccc cttgggctgc gaacccatcg tcgtgttgag cgacacttgc
     7741 gtgggcctgg agcggcttgc catggaatgg gagcgctgct ggcgcatgat ccgcatcgcg
     7801 cgcgatttcg gcaagagcgg cgtgctcggc atgcccgacc tggggccctt gcccatgctg
     7861 gtgggcgctg ccgactcggc cgacgtcagg gatttcatcc atggcgccat aggcaagctg
     7921 gtcgagtgcg atcagcagac ccattcaccc tacctggaaa ccctggccgc ctatctgcgc
     7981 tcgggttgcc gcggccaacc ctgcgccgat gcgatgggac tgcacgtgac cacgctgcgc
     8041 taccgcctgt cgcgcatcaa atccctattc ggcatcgacc tcgactctcc cgagcggcgt
     8101 tttgccatcg aactggccat caacctgcac aagctgacgc gcggcgagag tccggctacg
     8161 ggttcacccg aatccctcca tcgcacatga ggtgattttc catccgcctt cctccgaatc
     8221 ggtggaaggc ggcggcttcg cgatccccta ttgttgacct cggcgcagtg cagtgtcgac
     8281 ctgacccatg cgcttcgcgc acgtatcttt tcgagaaaac aatgagatcc tcccgccgaa
     8341 tgcatctggt cgcctatctg aagaccggac cgaccgctcg ccacgccggc ggctggcgtc
     8401 acccggaggc cgtgctggac gatttcctga gcccttccag gtacatccat ctcgcgaagc
     8461 ttctggagtc cgcgaagttc gacggttgct tctttgcgga ccttttcggc ctgtatgaca
     8521 tccacgacgg aaacttcgat gcctacgtcc gcaggggcgg ccagatcagc tatctcgacc
     8581 ccatgacggt cctgccgatc atggccgccg cgacgagcaa gctggggttg ggcgccaccc
     8641 tttcgacgac gttctacaac ccctaccacc tggcgcgggc gctcgcgtcc ctcgacgtgc
     8701 tcagcggcgg ccgcgcggcc tggaacgtcg tcacttccgc gacggaactg gaagcccaga
     8761 acgccggcat ggaccagctc ccctccaagg acgagcggta tgacagggcg gacgaggtgc
     8821 tggaagcctg catggccttg tggaacagct gggatgccga tccgttcctg ctcgacaagg
     8881 cgcaaggaac gtttgccgat ccgtccaagg tccactatgc caactacaag ggaaagtggg
     8941 tcaagacgag aggcccgctc tccataccga gaagcccgca ggggcgcccc gtgatcatgc
     9001 aggcgggcgg gtcggatcgc gggcggattt tcgcggcgcg ctgggccgaa gcgattttca
     9061 ccgtccagcg cgaccaggaa ggcatgcgcc gcttctacca ggaaataaaa ggcgggctcg
     9121 ccaagtgggg acgctccgag aacgactgcg caatactgcc agcggtatcc gtggtggtgg
     9181 gagaaacgga aagcatcgcg caggagcgag ccgaatacct gcgcagcctg gtcgacggcg
     9241 aactggtggc cgccagcgcg tccagcaatc ttggcgtcga catcaattcg gtggccagtg
     9301 aaaaggaact ggcgagcaag cagcgcaacc agggcatggc ggggtcggaa atcctcatca
     9361 agcaatccat ggcggccaat aacatcggct tcaaggaagc ggcgcagaaa tatcagcccg
     9421 acatgctggt cggcaccccg caatccattg ccgatcagtt gcagcattta ttcgaaagcg
     9481 aagtgtgcga tggattcatc gtgacgccga cctatagccc cggcatgctc gagcagtttt
     9541 gccgggcggt cgttccagaa ttgcaaaggc gcggggtttt cagaaaagaa tactcgggat
     9601 cgacgctgcg ccagcacctg caatccgatg ccgcttgaga ccgtgaggaa tctttcccgt
     9661 gactgatcga ctcaaaatcg agcatcttta caaggtcttc agcgatgcgc ccggcccggc
     9721 gctcgccctg ctgcgcagtg gcaggagcaa gaccgaggtg caggaacaac tgggccaggt
     9781 ggtcgggctg gacgatatct cgctggatat tccctccggc gcgatgtaca tgatcatggg
     9841 cctgtcgggc tccggcaagt cgacgctggc gcgctgcatc aaccgcctca acgaacccag
     9901 cgccggccgc atcctgctcg acgggcagga catctgcgcg ctcgacgaga cagggttgcg
     9961 ggaggtgcgg cgcaaccgca tctcgatggt gttccagcac ttcgcgctgc tgccgaaccg
    10021 ccgcgtcatc gagaacgtcg agttcgggct gaagctgcgc ggcgtgtccg ccaaggagcg
    10081 gcggcgccac gcgcaagagg tgctggaagt ggtggggttg gcgcgctggg cctatcacat
    10141 gccccagcaa ttgagcggcg gcatgcgcca gcgcgtcggc ctggcccgcg cgctggcgac
    10201 cgacgccgac gtgctgatca tggacgaggc cttcagcgcg ctcgacccgc tcatccgcag
    10261 cgaaatgcag gacgagctgc tgcgcctgca gcgcacgctc aagaaaacca tcctcttcat
    10321 cacccacgac ttccacgagg cgctcaagct gggcacccgc atcgccatca tggccgaggg
    10381 gcggctggtg cgcgaaggca cgccgcaatc catcgtgctc gaacccggca gcgactacgt
    10441 cgccgccttc acccgcgacg tcgaccgcgc ccgcctgttc gacgccgcct cggccatgac
    10501 gccgctgtcg gattggcgtg gcgacggttc ggcggccacc ggcgtcccgg acggcctggt
    10561 gtgcgtgccg ggcaccgcca agctcttcga agtggcggga aaatatgcat ccgcaggtcc
    10621 tcttctcggc gtcaccgacg acgcgggacg attgatcggc gtgctcgagg cgccgcaggt
    10681 gctggcccgc atcggccagg ctcaagccgc ggaggcgcac catgcttgac gccgaccaat
    10741 tggccttcct tcccgtcgat ctatggatac aaggcggcgt gaaatggctc gcgtccaacc
    10801 tgcgtccgct gttcctcacg atcaagtggc ccatcgagca gctgctggcc atgtgcgaca
    10861 ccgcgctgca cgccgtgcct ttcgtcgtcc tggtgctggt ggcggcgctc gtggtgtggc
    10921 ggctggccgg cgcggccgtg gcggccttca gcgccgtcgt gctggtcgtg atcgccatgc
    10981 tgggcgtctg gaacgaggcg atgaccacgc tgtccctcat cgtgacggcc atcgtctttt
    11041 gtgccgtgat cggcattccc atcggcattg cctgcgcgcg cagcgaccgg gtctggatga
    11101 ttgtccggcc catcctcgac gtcatgcaga cgacgccgac cttcgtctac ttggtacccg
    11161 tcgtcatgct gttcggcgtg ggcaccgtgc cgggcgaagt ggcggtggtg acggccgcca
    11221 tgccgccgct gatccgcttc acccagctcg gcatccgcat ggtcgaccac gagatcgtcg
    11281 aggccggcct ggcgttcggc gccgaccgcc gccaattgct ctgggaagtt caattgccgc
    11341 tggccgtgcc gaccatcctg ggcggcttga accagaccgt gctcacggcg atggtgatgt
    11401 cggtcgtggt ggcgatgatc ggcgccgagg gcctgggact ggtcgtgctg caaggcctcg
    11461 gccgcctgga cgtgggccgg gccgcggtcg gcggcatcgc catcgtgctg ctggcgatca
    11521 tgctcgaccg catcacgcag aagttgccga cgttcgaacc gtttgcccgg ctcggcctgc
    11581 tctcggtgat cgcccgcttc ctgcgcggcg gcaggccggc ggcagactcg gccgccaagc
    11641 gcgccgaaga catcaaaggt gtcctgtaat cctgttcacg caacttttca cgcacgaccc
    11701 gcatacaacc aggagtttcc aatgaaaaca tacgtcaagg gatacgcagc cgctctgctc
    11761 gccgtgctgg ccatcaacct gccggccgcg gcgcaagacc tgcccggcaa gggaaagacc
    11821 gtgcgctacg cgcagggaga cagcctgggc agcaactacg tcgtctccct gatcgtttcg
    11881 caggcgctga aggacatggg ctacggcgtc aagctgagca cgatgaacac caccctgttc
    11941 ttccaggccg cggcgcaagg cgaccaggac atagccacgg atatcaactt cccgcagcgc
    12001 gagctggcct tcaaggccgt ggcggatcag gagaccatca tcggcacggg cttgatcatc
    12061 ggcggcggcg tcaacggcta catgatcgac aagaaaaccg ccctggccca caacatcacc
    12121 agcgtggacc agttgaagga cccgaagctg gccggcctgt tcggcgccga cggcaaggcg
    12181 gagctgatca gctgcgaccc gggctggagc tgtggcgacg tggtcgacta ccagctggac
    12241 aagtttggcc tgaagcagac tgtcaagtcc gtgcgcggca agtacgaagc cctgatggtc
    12301 gacacgatcg ccaaagtgaa cagcgacaag ccggtgctct tctacacctg gagcccgtcc
    12361 tgggtgacca gcaccctcgt gccgggcaag gacgtggtgt ggctgcccac accgatggac
    12421 gccctgccgc ccaacatcac cgccagaggg tcggcgctgg tacccaacgt cgagggctgc
    12481 gccggcggcg ccaacccctg ccgcatggcc atggcgtcct ggaattgggg cgtcgtcgcc
    12541 aacaatgcct tcatcgcggc caatcccgcg gtcaaggccc tgctcgaaga gatcaagttc
    12601 ccgctctcga ccttctcgca ctgggaaggc gagatcggca agaatggcgg ctcccaggcc
    12661 acgatcaacc ggctttcaag cgagtggctg ggggcgaaca agacccagtt tgaccaatgg
    12721 gtggctactg ccgccaaggt ccattgatgc cgggagcggg ccatggcggc cacatcccac
    12781 ggcggtgctc cgcaatggat gtgaaaaact agagcaccgc atcggcggcg ccgcgcggca
    12841 gccgcgcggc gccctcctct tcttccagcc gggcgaccag ccgccggccg ctgcccaggc
    12901 aatgctcgga ggccatggcc tcggctttgt ccgcctcgcc cgcgcagatg gcttccacga
    12961 tggcgcagtg cgcctcgaac accagatcgt aggacggctg catggcagtc gtcgcggcga
    13021 tgtacaggcg tatctggtgg gtcaccggcg catagtgctg cgacagccgg cgatggcccg
    13081 acagatccac gatcagtttg tgcagtccca ggtccgcgct ggtcagggcg ctgtggtcgc
    13141 cctgccgcac cgcccggccc agggcatcca gtcccgcgcg cagcgccttg cggcgctcgt
    13201 cgtcgatgtt ctcggccgtc aaccgcacgc ccagcccctc gaagcgcgcg cgcagcgtgg
    13261 aaatctccca ggcgtcgtgc ggcgtcagct tcatcacgtg ccagcctgta taaggctgct
    13321 gcacgaccag gccctcggtg accaggcggt gcagcgccgc gcgcaccgtg ccacgtgaca
    13381 acgacaggcg ttcggccagc cggatctccg taagccgcgc accgggcggg aagacgcccg
    13441 tgacgatggc ctgacgcagg ctgtccgccg ccgcgcggtc caggctgcgc ttttccggcg
    13501 tgaccaggtc gtccagcttg ttctccattg ttagctcctc caagccctgc atagatcgat
    13561 tcccaaagca aaacgcctgc gtgcttgaca acacacagta ccgtctttta gagttaattg
    13621 gtgattgtca acaatcacca attgcttgta gcgtcttccc ccaagggtca atcgcgcgca
    13681 gcggactgtt ccggttttgc ttcatcactt ttggctattt gctgaatgtt gaatgttagc
    13741 agggcctctc tgccatgctg cgccgcacaa caacactacc ggagtcgttc catgacggac
    13801 gagacaaccg ttctttacac cgtcagcggc aaattctgca cgctgacgct caac
//
LOCUS       DASWPO010000151        25129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2533656, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000151 DASWPO010000000
VERSION     DASWPO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 25129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25129
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533656"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VGC69_08350"
     CDS             <1..951
                     /locus_tag="VGC69_08350"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0295326.1"
                     /translation="VPETLYPVRLLERDLVAWRDEDGALHVWEDLCVHRGARLSKGMI
                     HNNQVICPYHGWRYDVEAKCTMIPAAPKEAPMKKAQAFPYRVQERYGLVWVCLGEPAH
                     DIAVFPEWEDEAFLKVHCGPYAFAANGFRAIENFLDISHFPFVHSGMNGVPENPDSLD
                     PYSVEMTESGLMSSEIKVFQPFGDARGTPIISNYTFRSYRPLVGYFSKRTLDADAAGN
                     PVTEHCNYFTTYCAAQAIDETHCILRVCAALSVKPAPDPESVRRRADVIFGQDKEIVE
                     TQRPERIPSELRYELHHRSDLMGQRYRTWLRSLGITYGVI"
     gene            981..1907
                     /locus_tag="VGC69_08355"
     CDS             981..1907
                     /locus_tag="VGC69_08355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY0295327.1"
                     /translation="MRLDAIRYGARNINLYHFVPVSGGPLPAAEPGAHIDLRIGEGLT
                     RQYSLINAGDAPGEYVIAVQKDERGRGGSKAFHTASIVGEEYLISPPRNHFALTSRGS
                     PTFLFAGGIGITPLFNMYRSLKRDGHPVTLYYWARTAADFLFYDELRAAEDVHLIATD
                     PDPARVALLNDVIPAIADDAHLYCCGPAGMLAAFDAACAGRRGELIHQERFAAKDIAP
                     GTGTFEVRLARFGKTIVVDPDTSILQACLEAGADVSYSCEEGVCGACEVRVLAGKVVH
                     NDTVRSASAHDAEGTMMICCSRAAGASLTLDV"
     gene            1938..2588
                     /locus_tag="VGC69_08360"
     CDS             1938..2588
                     /locus_tag="VGC69_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0295328.1"
                     /translation="MSPRPKAYLFDFDLTLADSSAGIISSAQAALDQMDFPVPDADAV
                     RRSIGLPLEASFTFLTANHDAALAKTYSRYFVAHADKVMVAMTHFYPSVPPLLGHLRK
                     QGIRVGIVSTKFRYRIDAILRHAGLEPYVDIVVGGEDVARHKPHPEGIRKALGAIAVA
                     PADAVYVGDHIMDAQAARAAGTRFIGAVTGSIGYADWQAEGESAVRKDLSELLDIA"
     gene            complement(2633..3553)
                     /locus_tag="VGC69_08365"
     CDS             complement(2633..3553)
                     /locus_tag="VGC69_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295329.1"
                     /translation="MDNEIGWELYRTFLAVLTEGSLSGAARALGITQPTAGRHIAALE
                     AALDQTLFTRSQTGLMPTEAAQALRGHAETMHATAAALQRAATRHGQGIQGTVRISAS
                     EVIGMEVLPPTLARLRQAHPGLRIELAATNRLQDLLHREADIAVRMTAPRQDSLIARR
                     VGSVTIGLYAHPDYLARCGTPAMPADLKQHALIGFDVETPFLRAARTTLPAWMRDAWT
                     RDAFALRADSDLAQLAMVRAGCGIGICQVALARRVPPLTRVLPGQVAIPLETWITMHE
                     DLRGNPACKAVFDALVRCLLAHIAAGQAKA"
     gene            3650..4651
                     /locus_tag="VGC69_08370"
     CDS             3650..4651
                     /locus_tag="VGC69_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0295330.1"
                     /translation="MRNSGKTGTALVLGASGGVGGEALRQLRDAGWAVRALQRGPAGE
                     AAQRDGIIRLQGDAMNRDDVLRAARGCEVIVHAVNPPGYRRWGELVLPMIDNTIAAAV
                     AERATIVLPGTVYNYGPDAFPMLTEDAPQRPLTRKGAIRVELERRLRDAVRDGARAIV
                     LRAGDFFGPRARNNWFSQGLVKPGRPVGVVNQPGNPGVGHAWAYLPDVARTMVALLAR
                     RETLEPFARFHLAGHWDDDGTKMAAAIQGAAGRHGGRVKLKAFPWWLVPLAAPFVPTM
                     RELLEMRYLWREPVRLDNARLVGVLGAEPHTPWEEAVEATLAGLGCLGAGFHALSPP"
     gene            4849..5649
                     /locus_tag="VGC69_08375"
     CDS             4849..5649
                     /locus_tag="VGC69_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0295331.1"
                     /translation="MLRTIESLYGCPVVARDGEIGTVDQVYFDDEAWGVRYLVVETGG
                     WFNKRQVLISPYSIQHADPGSDAVHVDLTRQQVRDSPSIDTDKPVSRQHESQYLGYYG
                     YPNYWSGGYLWGVSANPLFGPMFPEQNLMLEERRQREQQEDEPPADVHLRGTKAVKGY
                     HIEAKDGNIGHVSGFIFDDVAWAIRYLVVDTRNWWPGSKEVLLATQWIGLVDWAGSTV
                     STELTRDAIKSSPPYDESALPSRSHEAALHQHYGKEGYWSKEESPPAS"
     gene            complement(5734..5810)
                     /locus_tag="VGC69_08380"
     tRNA            complement(5734..5810)
                     /locus_tag="VGC69_08380"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5774..5776),aa:Asp,seq:gtc)
     gene            complement(5879..5954)
                     /locus_tag="VGC69_08385"
     tRNA            complement(5879..5954)
                     /locus_tag="VGC69_08385"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5918..5920),aa:Glu,seq:ttc)
     gene            complement(6005..6080)
                     /locus_tag="VGC69_08390"
     tRNA            complement(6005..6080)
                     /locus_tag="VGC69_08390"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6045..6047),aa:Ala,seq:ggc)
     gene            complement(6307..7473)
                     /locus_tag="VGC69_08395"
     CDS             complement(6307..7473)
                     /locus_tag="VGC69_08395"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295332.1"
                     /translation="MAQAVPAWCAGLIDITRHALLGCKNNYHFRIDIQTRIQRPRLGP
                     HPAPVAAPVQETVINTTADSSRRALLRAALAAPALAAAGRAAAQGLAAAPIHVVVPFP
                     AGGSSDTLGRLVAAALAAQLKQSVIVENKGGAGGNIAADYVFRAQPDGHTLLVAGQAI
                     LAINEALYDHISYRPEAFEYLGMLGQNANVILANTASLSVHDLAGLIEQAKARPGAIS
                     FGSNGVGSLSHLTAEVLASAAHVRFLHVPYQGAAPMATDLRAGRLNFCVTGSTLATSL
                     MQGSDTLRALAVTTGVRIPQLPDTPTLRELGYPQLDAPSWWALMATPGTPEATLAQLR
                     TALAAAVGAPQFIQALAKQSTLPYRLDPHDAPAFLANERAKWAAAVKSSGAKLS"
     gene            7486..8262
                     /gene="tam"
                     /locus_tag="VGC69_08400"
     CDS             7486..8262
                     /gene="tam"
                     /locus_tag="VGC69_08400"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEY0295333.1"
                     /translation="MSSVIDQYAKQYVMFESERTRPVRDLVAAIPNDHVTVAADLGCG
                     PGNSTEVLAARYAQARIVGVDSSADMIASARKRLPGLRFELMPIEDWRGESACDVILA
                     NASLQWVPDHAALMPRLVAQLAPGGSLAVQMPDARGEPAQGLMRALAGQGPWAAKLKD
                     VERVPVDSVRGYYTLLKPHCSRIEVWRTTYHHVLAGGAPAVVEWFKASALRPYLKRLD
                     TAEQAEFLERYTAAVAEAYPALDDGSVLLPFPRLFFVATR"
     gene            complement(8270..9877)
                     /gene="lnt"
                     /locus_tag="VGC69_08405"
     CDS             complement(8270..9877)
                     /gene="lnt"
                     /locus_tag="VGC69_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0295334.1"
                     /translation="MSTPLRQRIGRPALLTLAGALHALTFAPGPLPDGVLALAQVLAL
                     AVLAWATLHSARPRQALLQGWWFATVYFSLGLYWLYISMHDYGDLSAPLAAAGVLTLS
                     AFLALFPGAACALARRLLPDPARTPWRAALIWAAAWTLLEWLRATVLTGFPWLNIGYA
                     QVDSPIAGWAPVLGVHGMALVAACAAAALAGLWPRRGKPTRGALLALALPLVLVLLGW
                     HLRGIAWSQPVGRPLHVRMVQGNIPQSQKFDPAMLRQGFALHFDLALRPPADGEPAPD
                     VIILPETVLPIFQDDIPPRVWDAWRAVAGRQHAALIMGVPLHTFGPQGERYTNSAIGL
                     TADTPTEDLLRGQTAQRYEKHHLVPWGEYVPPGFHWFINMLNMPLGDFDNGPERQKPF
                     AIADQHLALNICYEDLFGPELLPALRPVDGEPGATLLLNLSNLGWFGDTWALRQHLQI
                     GRLRTLETARPMLTDTNTGLTTAIDARGRVQASLPPHQAGVLAVTVQGMQGLTPYARY
                     GDLPIVLIIAAVLLAGAAGAWRRRNPP"
     gene            complement(9874..10764)
                     /locus_tag="VGC69_08410"
     CDS             complement(9874..10764)
                     /locus_tag="VGC69_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY0295335.1"
                     /translation="MSDPYPAADATTRPAKSSGRSLLDRVLSLVRREPEDREGIKAFL
                     DAAHERQLLDAEAYTMIKGALAVSERTVADIMVPRSRMDLLDITQPLPQLLATIIEAA
                     HSRFPVYEHDRDNIIGILLAKELLRCMLEPGLDIRALLRPAVFIPEAKRLNVLLHEFQ
                     EGSTHQAIVIDEHGGISGLVTMEDVLEQIVGDIEDEFDEDEEESIFPEGEGQWRLMAS
                     TQISHFNEVFHTTLPDDEYDSVGGWLGGQLGRIPKRGDNAAYGDLRLEVIRADARRAL
                     WLRARRLPQPRSALASTATE"
     gene            complement(10830..11375)
                     /gene="ybeY"
                     /locus_tag="VGC69_08415"
     CDS             complement(10830..11375)
                     /gene="ybeY"
                     /locus_tag="VGC69_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY0295336.1"
                     /translation="MKTGSKRSDAARAALAVPREGRAGLGAALSLTVQYGPAEPRLPR
                     WRLRNWAGYALQAAAEDGQAVFGRAELNLRLVGLAEGRKLNRDFRGRDYATNVLTFEY
                     GVDPKGTAHGDIIICAPVLAREAREQKKPFLHHAAHLTIHGVLHSLGYDHLNKRDAQR
                     METLEIQVLARMNIADPYTIT"
     gene            complement(11359..12363)
                     /locus_tag="VGC69_08420"
     CDS             complement(11359..12363)
                     /locus_tag="VGC69_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0295337.1"
                     /translation="MPVIVNLDGDNTHLANLCGPLDENLRQLADGMGITLSRRGSRIT
                     LEGGQAELAGRALRRFFDQATHKALSIDDIQLGLVEIGIVRPAEAPAIPTEAPTLDDN
                     GLPSLADEDSIPLRTRRSDLRPRTPRQRDYLNNILKHDITFGVGPAGTGKTWLAVACA
                     IDAMERETVQRLVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFDRVQK
                     LFEKQTIEIAPLAYMRGRTLNHAFVILDEAQNTTPEQMKMFLTRIGFGSKAVITGDPS
                     QVDLPRGQQSGLAHAVQVLEDVQGIACTRFTSRDVVRHPLVARIVDAYDKASADEDRQ
                     "
     gene            complement(12444..13913)
                     /gene="miaB"
                     /locus_tag="VGC69_08425"
     CDS             complement(12444..13913)
                     /gene="miaB"
                     /locus_tag="VGC69_08425"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY0295338.1"
                     /translation="MQETTLSRPGSKSGGKTKSIQGKSGEPIVSTAATATGGAPRKVY
                     IRTFGCQMNEYDSDKMLDVLRAEHGLEPTQTPEDADVILLNTCSVREKAQEKVFSDLG
                     RANQLKKANPNLVIGVGGCVASQEGEAIVRRAPYVDVVFGPQTLHRLPELIDKRRALG
                     ASQVDISFPEIEKFDNMPPPRVEGATAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFDD
                     VLVEVADLADQGVREVTLLGQNVNAYRGKMSDTGEIADFATLLEYVHEIPGIERIRYT
                     TSHPKEMTQRMVEAYARLPKLVSFLHLPVQAGSDRVLAAMKRGYTALEFKSVVRKLRA
                     ARPGLTLSSDFIVGFPGETEEDFEKTMKLIEDVGFDTSFSFVYSRRPGTPAADLHDDT
                     PQDIKLRRLQRLQARINEQAAAIAQGMVGTRQRILVEGTSRRDDKELMGRTENNRIVN
                     FASPESTHPRLIGQMVDVVITQAYPNSLRARVADVDAAR"
     gene            complement(13998..14384)
                     /locus_tag="VGC69_08430"
     CDS             complement(13998..14384)
                     /locus_tag="VGC69_08430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0295339.1"
                     /translation="MTRRQAIYVKGFAHNNPIPAACRIGNIVTTGVINGVDPATGEFP
                     DTLEQQCVFLFQQVDIIVRTAGGSMDDIIKMTVWMKDSKARAALNEVWLSVFPDADTR
                     PARHTMQAALEGKRLIQCDFTAILGD"
     gene            complement(14400..15206)
                     /gene="hpaI"
                     /locus_tag="VGC69_08435"
     CDS             complement(14400..15206)
                     /gene="hpaI"
                     /locus_tag="VGC69_08435"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HEY0295340.1"
                     /translation="MEISRNLFKQALSENKAQIGLWVGLADAYVAELLAITGFDWLLI
                     DGEHAPNDLRSTLAQLQAVAPYPSHPVVRPPIGDVALIKQVLDVGAQTLLIPMVETAE
                     QAAQMVAATRYPPSGIRGVGSALARSSRWNQIPDYLHRADAEMCLLVQIESAAGLRNL
                     EAIARTPGVDGVFFGPADLSASLGHLGNPVHPEVQDAIMEGIKQVRAAGKAPGILTSD
                     AKLARAYLDAGALFVAVGVDTTLLVNSARRLASSFKHAPEPEANGPSSVY"
     gene            complement(15223..16026)
                     /gene="hpaH"
                     /locus_tag="VGC69_08440"
     CDS             complement(15223..16026)
                     /gene="hpaH"
                     /locus_tag="VGC69_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEY0295341.1"
                     /translation="MLSPDTISALAQQLYQARKTRTQLRHFSTAHPDMTIDDGYRIQA
                     EWVRLEQVDGRTIRGRKIGLTSRAMQLSSQINEPDYAPLMDDMFFETGGDIPYSRFIA
                     PRVEVELAFLLREPLRGPGVTLYQVLAAAEYVVPAIEIIDSRIEQFDRDTKAPRKVFD
                     TISDFAANAGVVLGGRPVKPDSIDLRWVGALLHRNGVIEESGLAAAVLNHPANGIAWL
                     ANKIARHDEQLNAGDIVLAGSFTRPTPAQPGDTFHVDYGPLGSVSFRFI"
     gene            complement(16089..16847)
                     /locus_tag="VGC69_08445"
     CDS             complement(16089..16847)
                     /locus_tag="VGC69_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0295342.1"
                     /translation="MKRARVIYQSQTYEATDAGGGKVRLSDGRVLDELEVSWLPPMEF
                     GTIFVLALNYADHAKEIAFNKAPDAPVVFLKGPGSFTGHRTTTRRPADVNYMHYECEL
                     AVVIGTRCKNVPRERAYEVIGGYTVANDYAIRDYLENYYRPNLRVKSRDATTPIGPWL
                     VDAADVPDPMNLRLRSWVNGHPTQDGNTRDMVFDIPALVAYLSSFMTLNPGDVILTGT
                     PDGVSDAAVGDEIITEVEGIGRLVNTIAADDLPD"
     gene            complement(16844..17506)
                     /locus_tag="VGC69_08450"
     CDS             complement(16844..17506)
                     /locus_tag="VGC69_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0295343.1"
                     /translation="MANRTFLPRIVYGTLLNDRATLARMANAFTQPPYKAPPVHPVLY
                     IKPANTYAPPGARIEVPADPGVVRIDATLGVVMRHDARRVEASNAMDYVGGYLIASDI
                     TLPHDDLYRPPLLQRCRDGFCPMTPPLHAGPDFEPGKAVIDIAINGHLAHRRSLQDLC
                     RPIAQLIADVSADMTLAAGDVLLVGTPEGAPLARPGDRIRIEVAGLGSLEHELIAEGA
                     AA"
     gene            17728..18189
                     /gene="hpaR"
                     /locus_tag="VGC69_08455"
     CDS             17728..18189
                     /gene="hpaR"
                     /locus_tag="VGC69_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HEY0295344.1"
                     /translation="MATDEMREGLRAFSRSLPMALLRARESVMGRFRPMLRDHDLTEQ
                     QWRVLRALNAAKAPLSANELSDQTFISMPSLSRLLRTLEARGVIERTAHHEDLRVAQL
                     SINRVGRELVAVVGPLSEASYGEITEQIGEHELEQLERLLNHVVARLGLPR"
     gene            complement(18214..19362)
                     /locus_tag="VGC69_08460"
     CDS             complement(18214..19362)
                     /locus_tag="VGC69_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0295345.1"
                     /translation="MQSHSIAEIHAFPVSYPLPPHAQPGMGTGRVVRRDAVVVKVVTA
                     SGLVGWGECFHARSHMAIATLVNAYLHPLLQGMDATAAVDVWSKVYNYHLAGYGAGAA
                     CYIALSGIDLALWDIRGKVAGMPLYQLLGGSRRRVPAYAGGLSLGFEEPAKLIERVRA
                     LADAGYSAVKLRVGDTPERDIERLRAIRAAFPALTVMTDANTAYSLAAAQRVLPVMDE
                     LGIGWLEEPFPMDHREAYRQAARNARTPLAAGENLYGKVDFARLIEEGSVQVLQPDLS
                     KSGGITECLRIAALAAAHKLPVHVHSSMTGINQAASIHFLASIENPGYFEADVSEGNK
                     FRDELVTTPYRVEADGCVLPNDRPGLGIDVDESFFDRYPAAQGLTNKV"
     gene            complement(19377..20357)
                     /locus_tag="VGC69_08465"
     CDS             complement(19377..20357)
                     /locus_tag="VGC69_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295346.1"
                     /translation="MKILTKIIAAAVSTAALFGACAHAADSYPGKPIRLVVGFAPGGA
                     TDVLARTVASRLSEKLHGNVVVENRTGAGGLIAGNFVAKSAPDGYTLAFASTGAFSIG
                     PSLYKKKLPYDPIKDFTPVALVSQNTHVLVVNPSVPAKTVAELVAYAKSKPGALSYGS
                     FGNGSTSHLLAEQFKKLAGVDAVHIPYAGSPAMVTATMTGDVAFAVDTLQSSLPYIRS
                     GKLRALAVISPDRSPVAPEIPTFKEAGYADIDLKNWYGIVAPAGTPAEVVDILAKNIT
                     EIVGSPDFRDFVSRQGGETATAQGPEFGAFLAVQEKRWGGLVKASGATVD"
     gene            complement(20376..21497)
                     /locus_tag="VGC69_08470"
     CDS             complement(20376..21497)
                     /locus_tag="VGC69_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0295347.1"
                     /translation="MAIAAIHTIPVLIPFDVDGPKQEFLGQPRSHIEALYLKVETSDG
                     YTGWGEAFGFNLWPVIRKAMDDFIAPFIIGRDENDIDALMTELRRRFHVFGRTGPVNY
                     ALSGLDIALWDIAGKKAGKPLAELFGGARRDSLPAYASLMKYGEADALARNCAHAKDL
                     GYSAVKIHESTVRLAQIARDSLGAGPGLMVDTNCAWTLEQACSYLPALQAMNLLWLEE
                     PTWPPENLDNFRVLRERADIPLAAGENAATPWELAAMSRSGLLDVVQPSVIKIGGISE
                     MRAAYQALTPLSKTQGSARAQPIAHSPYFGPGLFATLQLAAALEQESPLEKLFCTFEA
                     HPYGDAIEVVNGRLRVPTGPGLGIDPDPQILQAFSVTQP"
     gene            complement(21515..22489)
                     /locus_tag="VGC69_08475"
     CDS             complement(21515..22489)
                     /locus_tag="VGC69_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295348.1"
                     /translation="MRSFRTLLGIAVAVLYAAAPLAAQAQQAPGTIRLIVPYVPGGST
                     DTVARLIAPYIGKALGATAVVENRAGANGTIGLQAVARATPDGTTFGVTDSAFVVSPS
                     LSKIPLPYDTRKDFKPVVLMAASPQVLTINPQIPARTIKELVTLAKQSPGKYSFASAG
                     PGTAIHIAGEQLKVSAGIDILHVPYRGLGPAMTDVISGQVSMVFGGPHTTRQQVAAGN
                     LRALAITGPHRSPAMPGVMTFAEAGYPGVDMVTTNGLVAPAGTPDAIVNKVNQAVIQA
                     LKDPELKRHFDELGLEPIGDTPQEFTAWIDSQLDKWATLVKQANIQVD"
     gene            22897..23883
                     /locus_tag="VGC69_08480"
     CDS             22897..23883
                     /locus_tag="VGC69_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295349.1"
                     /translation="MTLQRRIALKLTQGLLALAACLAATLAQADYPDHPITFISAYVP
                     GGTNDFLTRFIAQGVGKDLNSSTIVENKPGANGIVGTTFVSRSAPDGYTLLMGNSASH
                     GINPTLYPNLQYDAVKGFQGVSMVASVPIVLVVNAKLPVHNLKELVEYGKSHPGRLSF
                     GSSGTGGAGHLAGEAFILSSGIDMVHVPYKGDAAAVADTMAGQIPACFVALSSATPQL
                     ASGMLKVLAVASDKRLAKYPDVPTMDESGYPNMEFAQWFAIVAPAATPRPVIEKLNLS
                     IKRVLESPAARSSFDTQGAEPVYTTPEQTKAFIESEIQRFGSIIHKLNLHAN"
     gene            23909..25090
                     /locus_tag="VGC69_08485"
     CDS             23909..25090
                     /locus_tag="VGC69_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0295350.1"
                     /translation="MNAKVAKIETLSCDAGWRNYHFVKLTTEDGIVGWSEFDERRGVP
                     AVAAIIEQLGQGLIGAPVGAHERFHAQASAATRTAVRGVVSQAIGAIENAMLDAKART
                     LGVPCYELLGGKIRDRLRVYWSHCPTWRIAHPQYYSPAVTDLDGVKAAAREARERGFG
                     ALKTNMFIHDEGPAYFWRPAFGSPFQPEVNVDRKVLRNVRGHLEALRDGAGPEMDILL
                     DLNFNARTEGHLKLLRAIADLDIFWVEIDCFEPNALAYIRDHSPHPISSGETLFGVRD
                     FLPFFRQDAMDVAIIDAVWNGVWQSMKIAALADGCEINIAPHNFYGHLCTMMNAHFAA
                     AVPNLRIMEADIDRLAWDDELYTHAPEYHDGHLIIPDRPGWGTEPNEDALRAHPARSE
                     K"
BASE COUNT         4104 a         7672 c         8829 g         4524 t
ORIGIN      
        1 gtgccggaaa cgctctaccc ggtgcggctt ctggagcggg acctcgtcgc ctggcgcgat
       61 gaggacggcg ctctccatgt ctgggaagac ctgtgcgtcc accgtggcgc ccgcctgtcc
      121 aaagggatga tccacaacaa ccaggtgatc tgtccctacc atggctggcg ctacgacgtc
      181 gaggcgaagt gcacgatgat tcccgccgcg cccaaggaag cgccgatgaa aaaggcccag
      241 gcgtttccgt atcgggtcca ggaacgctac ggcttggttt gggtctgcct tggcgaaccc
      301 gcgcacgaca ttgcggtgtt tcccgagtgg gaagacgagg ccttcctgaa ggtccactgc
      361 ggtccgtacg cgttcgccgc caatggcttt cgcgcgatag aaaactttct ggacatctcg
      421 cactttccct tcgtgcattc gggcatgaac ggcgttccgg aaaatccgga cagtctcgat
      481 ccctactcgg tcgagatgac cgagtcgggt ttgatgtcgt ccgagatcaa ggtcttccaa
      541 cccttcggcg acgcccgggg cacccccatc atttccaact acacgtttcg cagttaccgg
      601 ccgctcgtcg ggtatttctc gaagcggacg ctggacgcgg atgcggcggg caaccccgtc
      661 acggagcatt gcaactactt tacgacctac tgcgccgcgc aagcgatcga cgaaacccat
      721 tgcatcctgc gggtatgcgc cgcgctgagc gtgaagcccg cgccggatcc ggagagcgtg
      781 cgccggcgag ccgacgtgat cttcggccag gacaaggaga tcgtcgaaac gcagcgcccc
      841 gagcggattc ccagcgagct gcggtatgaa ctgcatcacc gctccgacct gatggggcag
      901 cgttatcgga cctggctgcg ttcgctcggc atcacctatg gcgtgatctg aaggcgatgc
      961 attccagcac cagcctgcgc gtgcgcctgg acgccatccg gtacggcgcg cgcaacatca
     1021 acctctacca ttttgtcccc gtgtccggcg ggccgctgcc cgccgccgag cctggcgcgc
     1081 atatcgacct gaggatcggc gaggggctca cgcgccaata ctcgctcatc aacgccggcg
     1141 atgcacccgg cgagtatgtg atcgccgtcc agaaggacga acggggacgc ggcgggtcca
     1201 aggcctttca caccgccagc atcgtcggcg aggaatacct gatttcgccg ccgcgcaatc
     1261 atttcgccct gaccagccgc ggcagcccca cttttctgtt cgccggcggc atcggcatca
     1321 cgccgctgtt caatatgtat cgctccctca agcgcgacgg ccatccggtg acgctctatt
     1381 attgggcgag aacggcggcc gatttcctgt tttacgacga gttgcgcgcg gccgaagacg
     1441 ttcatcttat cgcgaccgat ccggatccgg cccgggtcgc gctgctgaac gatgtcattc
     1501 ctgccattgc ggacgacgcg cacttgtact gctgcggccc ggccggcatg ctcgcggcct
     1561 tcgacgcggc atgcgcggga cggcgcgggg aactgataca ccaggagcgg ttcgccgcga
     1621 aggatatcgc gcccggtacc ggtaccttcg aagtcaggct ggctcgattc ggcaaaacaa
     1681 tcgtcgtcga cccggacacg tcgatactgc aggcatgcct tgaggccggc gcggatgtct
     1741 cctattcttg cgaagagggc gtgtgcggcg cctgcgaggt gcgcgttctt gccggcaagg
     1801 tcgtgcacaa cgatacggtg cgctccgcga gcgcccacga cgccgagggt acgatgatga
     1861 tctgctgctc gcgcgccgcc ggcgccagcc tgaccctcga cgtttgaact caacccgcca
     1921 ccctgcggag cccaggcatg agcccacggc ctaaagccta tctattcgat ttcgatctca
     1981 ccctcgcgga ttcgtccgcc gggatcatct ccagcgcgca ggcggcgctc gaccagatgg
     2041 atttccctgt gccggatgcg gacgcggttc gacggtcgat cggcctgccg ctggaagcca
     2101 gctttacctt cctgaccgcg aaccatgacg cggcgctggc caaaacctat tcccgatact
     2161 tcgttgccca cgccgacaag gtcatggtgg ccatgacgca tttctatccg tcggttccgc
     2221 cgctgctcgg acacctgcgc aagcagggca tccgggtggg aatcgtctcc accaagttcc
     2281 gctaccgcat cgatgcgatc ctgcggcacg cgggacttga accctacgtg gacatcgtcg
     2341 tcgggggaga agacgtcgcc cggcacaagc cgcatcccga gggtatccgg aaagcgctgg
     2401 gggcaatcgc ggtcgctccc gctgacgccg tctatgtcgg cgatcacatc atggatgcgc
     2461 aggcggcgcg cgcggccggc acgcgcttta tcggcgcggt gaccggatcg atcggctacg
     2521 cagactggca ggccgaaggg gagagcgcgg tcagaaagga tttatccgag ctcctggata
     2581 tcgcctgagg cgaaccgggc ggcgcggatg atgtttgggc cgccctgctt tttcacgcct
     2641 tggcttgccc ggcggcgatg tgcgccagca ggcaccgcac gagcgcgtcg aacacggcct
     2701 tgcaggccgg gttgccgcgc aggtcctcgt gcatggtgat ccaggtttcc agcggaatcg
     2761 ccacctgccc gggcagtacg cgcgtcagcg gcgggacgcg ccgggccagg gcgacctggc
     2821 agatgccgat gccgcagccc gcgcggacca tggccagctg cgccaggtcg ctgtcggcgc
     2881 gcagcgcgaa agcgtcgcgt gtccaggcat cgcgcatcca ggccggcagt gttgtccgcg
     2941 ccgcgcgcag aaagggcgtt tccacgtcga agccgatcag cgcatgctgc ttcaggtcgg
     3001 ccggcatggc gggcgtgccg cagcgcgcca ggtagtcggg gtgcgcgtag aggccgatcg
     3061 tcacgctgcc cacgcgccgc gcgatgagcg agtcttgcct gggcgcggtc atgcgcaccg
     3121 caatgtcggc ctcgcgatgc agcagatcct gcaggcggtt ggtggctgcc agttcgatgc
     3181 gcagaccggg atgcgcctgc cgcagtcgcg ccagcgtagg cggcagcact tccatgccga
     3241 tcacttcgct ggccgagatg cgcacggtgc cctggatgcc ctgcccgtgc cgcgtggcgg
     3301 cgcgctgcag cgcggcggcc gtggcgtgca tggtctcggc atggccgcgc agcgcctggg
     3361 cggcttcggt gggcatcagc cccgtctgcg agcgggtgaa cagcgtctgg tccagcgccg
     3421 cttcgagcgc ggcgatgtgc cggcccgccg tgggctgggt gatgccgagc gcgcgcgccg
     3481 cgcccgagag cgagccctcc gtcagcaccg ccagaaaggt gcggtacagc tcccagccaa
     3541 tttcattgtc catacataaa tgtatagctg ataggctatg ttcagtaatt tattttgttc
     3601 atgcccaggc ccagaatgcc tgccatacgg aacagccagg agcatggaca tgcgtaattc
     3661 aggcaagacg gggacagccc tggtgttggg cgccagcggc ggcgtcggcg gcgaggcctt
     3721 gcgccagttg cgcgacgcgg gctgggcggt ccgggcgctg caacgcggcc cggccggcga
     3781 ggcagcgcag cgcgacggca tcatccggct gcaaggcgac gcgatgaacc gcgacgacgt
     3841 attgcgggcc gcgcggggct gcgaggtcat cgtgcatgcg gtcaacccgc cgggctatcg
     3901 ccgctggggt gaactggtgt tgcccatgat cgacaacacc atcgcggcgg ccgtggccga
     3961 gcgcgccacc atcgtgctgc cgggcacggt ctacaactac ggcccggatg cttttcccat
     4021 gctgaccgaa gacgcgccgc agcgcccgct gacccgcaag ggcgccatcc gcgtcgagct
     4081 ggagcgccgc ctgcgcgacg ccgtccgcga cggcgcgcgg gccatcgtgc tgcgggccgg
     4141 cgacttcttc gggccgcgcg cccgcaacaa ctggttttcg caagggctgg tcaagccggg
     4201 gcgtccggtc ggcgtcgtca atcaacccgg caatccgggc gtgggccacg cgtgggccta
     4261 cctgccggac gtggcgcgca cgatggtcgc gctgctggcg cggcgcgaaa cgctggaacc
     4321 gttcgcccgt tttcatctgg cgggtcactg ggacgacgat gggacgaaga tggcggcggc
     4381 catacaaggc gcggccgggc ggcatggcgg acgcgtgaaa ctgaaagcct tcccctggtg
     4441 gctggtaccg ctggccgcgc ctttcgtgcc gacgatgcgc gagttgctgg agatgcgtta
     4501 tctgtggcgc gagcccgtgc ggctggataa cgcgcgcctg gtcggcgtgc tgggcgccga
     4561 gccgcatacg ccgtgggagg aggcggtgga ggccacgctg gccggtctgg ggtgtttggg
     4621 agccggattc catgcgctat cgccgccctg aatggttaag aatttttggg catggcatgg
     4681 caaccgtcag ccgatgcagg ggcaaatgga agtgccaagt ccggcgtaat gggttcggca
     4741 ccgtactgac tcgcatgccg gttgaacgta gtgtttttcc atcacgttaa agctcgttcg
     4801 agcatgcgcg acccgctgga tggcgggaat ggaaaaggag tttcagatat gttgagaacc
     4861 atagaaagtc tctacggatg cccggtcgtc gcacgcgatg gcgagatcgg gacggtcgac
     4921 caagtctatt tcgatgacga ggcgtggggt gtgcgctatc tcgtggtcga aaccggaggt
     4981 tggttcaata agcggcaggt attgatttca ccctattcga tccaacatgc ggatcctggt
     5041 tcggacgccg tgcacgtgga ccttacgcgg cagcaggtca gggatagccc aagtatcgac
     5101 acggataagc cggtgtcgcg ccagcacgaa tcccaatacc tcggttacta cggctatccg
     5161 aattactgga gcggtggata cctatgggga gttagcgcca atcccttgtt cggaccgatg
     5221 tttccggagc agaacctcat gttggaggaa cgcaggcagc gagagcagca ggaagacgag
     5281 ccgccagcgg atgttcatct gcggggcacg aaagcggtaa agggttatca catcgaggcc
     5341 aaggatggca acataggcca cgtctccggg tttatcttcg acgacgtggc atgggctatc
     5401 cgttacctgg ttgtcgatac gcggaactgg tggcctggta gcaaggaggt gctgttggcg
     5461 acgcaatgga tcggtctggt tgactgggct ggatccacag tgtccaccga gctcacacgc
     5521 gatgccataa aaagcagccc gccttacgac gaatccgcgc taccgagccg tagccacgag
     5581 gcagcgcttc atcaacacta tggcaaggag ggctattggt cgaaagagga aagcccgcct
     5641 gcgagctgaa tcctgctgga ttcatgttgt gtggccagaa aagcaaaagc cctgatttct
     5701 cagggctggt cgctaggcga tgcatccaag ctttggcgga gcggacggga ctcgaacccg
     5761 cgacccccgg cgtgacaggc cggtattcta accaactgaa ctaccgctcc gcagcggtat
     5821 tgcgtcactg tgtagcggat gcaggcagcc aggcttcatt cggtactgcc gtaccgagtg
     5881 gcgtccccta ggggattcga acccctgtac tcaccgtgaa agggtgatgt cctaggcctc
     5941 tagacgaagg ggacagcgga cgcgcattac tactactgca acaactacgt tcttcgcttt
     6001 cgactggtgg aggtaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct
     6061 cccagctgag ctataccccc ggtgccttac taatgcgtcc tcgcgcttgc ttttctgctt
     6121 ccaccgtggc gccttttttt ataggccttt ttagcctact gccgctgcgg tttctgcttc
     6181 cctgcttttg ctcggttgct tcgtttcggt taatgccgtt ggcgaagaaa cgagattatg
     6241 cacgagtttt ttggggcgtg caagttgagg gccgggcctg gaaaaagggt gccagggcaa
     6301 ggctggtcag gacagtttgg ccccgctgct cttgaccgcg gccgcccatt tggcgcgttc
     6361 gttggccagg aaggcgggcg cgtcgtgggg atcgagccgg tagggcaggg tggactgttt
     6421 ggccagggcc tggatgaact gcggtgcgcc gacggcggcg gcgagcgcgg tgcgcagctg
     6481 ggccagcgtt gcctcgggcg tgccgggcgt ggccatcagg gcccaccacg agggggcgtc
     6541 cagttgcggg tagcccagtt cgcgcagggt gggggtgtcg ggcagttgcg ggatgcgcac
     6601 cccggtcgtg acggccaggg cgcgcagggt gtcgctgcct tgcatcaggc tggtggccag
     6661 cgtggacccg gtgacgcaga aattgagccg gccggcgcgc aggtcggtgg ccatgggggc
     6721 ggcgccctgg tagggcacgt gcaggaagcg cacgtgggcg gcgctggcca gtacttcggc
     6781 cgtcaggtgg ctgagcgagc ccacgccgtt ggagccgaag ctgatggcgc ctgggcgggc
     6841 cttggcctgc tcgatgagcc cggcgaggtc gtgcacggac agggacgcgg tgttggccag
     6901 gatgacgttg gcgttctggc cgagcatgcc gaggtattcg aaggcctcgg ggcggtagct
     6961 gatgtggtcg tacagggcct cgttgatggc caggatggcc tggccggcca ccagcagcgt
     7021 gtggccgtcg ggctgcgccc ggaatacata gtcggccgcg atgttgccgc cggcgccgcc
     7081 cttgttttcg acgatgacgg actgcttgag ctgcgcggcc agggcggccg cgaccagccg
     7141 gcccaaggta tcggaggagc cgccggcggg aaagggcacg acgacatgga tgggagcggc
     7201 ggccaggcct tgcgcggcgg cgcggcccgc cgcggcgagc gcgggggcgg ccagcgcggc
     7261 acgcagcagg gcgcggcgcg atgagtcggc ggtggtgttg atgactgtct cctgtacggg
     7321 tgcggcaacc ggtgctggat gcggcccgag tcttggtctc tgaatcctgg tctggatgtc
     7381 gattcggaag tgataattgt tcttgcatcc tagaagcgca tggcgcgtaa tgtcaatcag
     7441 acccgcgcac catgcaggga ctgcctgagc catgaggagg tggccatgtc ttccgtcatt
     7501 gaccagtacg ccaagcaata tgtgatgttc gaaagcgaac ggacgcggcc ggtgcgcgac
     7561 ttggtggcgg ccatccccaa cgaccatgtc acggtggcgg cggacctggg ctgcggcccg
     7621 ggcaattcca ccgaggtgct ggccgcgcgc tatgcccagg cgcggatcgt gggcgtggac
     7681 tcttccgccg acatgatcgc gtcggcccgc aagcgcctgc ccggcttgcg cttcgagctg
     7741 atgcccatcg aggattggcg tggcgagtcg gcctgcgacg tgatcctggc caatgcctcg
     7801 ctgcagtggg tgcccgacca cgcggcgctg atgccgcgcc tggtggcgca gctcgcgccg
     7861 ggaggcagcc tggccgtgca gatgcccgat gcccgcggcg aacccgcgca ggggctgatg
     7921 cgcgcgctgg cgggccaggg tccgtgggcc gccaagctca aggatgtcga gcgcgtgccg
     7981 gtcgattcgg tgcgtgggta ctacaccttg ctcaagccgc attgcagccg catcgaagtg
     8041 tggcgcacca cctaccacca tgtgctggcg ggaggcgcgc cggccgtggt cgaatggttc
     8101 aaggccagcg cgctgcggcc ctatctgaag cggctggaca cggccgagca ggcggagttt
     8161 ctcgagcgct atacggccgc ggtggccgag gcctatccgg cgctcgacga cggcagcgtg
     8221 ctgctgccgt ttccgcgctt gtttttcgtg gcaacgcgct gaaggcagct cagggcgggt
     8281 tgcgccggcg ccaggcgccg gccgcgccgg cgaggaggac cgcggcgatg atgagcacga
     8341 tgggcaggtc gccgtatcgg gcgtagggcg tgaggccctg catgccctgc acggtgacgg
     8401 ccaatacgcc ggcctggtgc ggcggcaggc tggcctgcac gcggccgcgc gcgtcgatgg
     8461 ccgtggtcag gccggtgttg gtgtcggtca gcatgggccg cgccgtttcc agcgtgcgca
     8521 ggcggcctat ctgcaggtgt tggcgcaagg cccaggtgtc gccgaaccac cccaggttgc
     8581 tgaggttcag cagcagggtg gcgccgggct cgccgtcgac gggccgcagg gcgggcagca
     8641 gttcggggcc gaacagatcc tcgtagcaga tgttcagcgc caggtgctgg tcggcgatgg
     8701 caaagggctt ctggcgttcg ggcccgttgt cgaagtcgcc cagcggcatg ttcagcatat
     8761 tgatgaacca gtggaatccc ggcggcacgt attcgcccca gggcaccagg tggtgcttct
     8821 cgtagcgctg tgcggtctgc ccgcggagca ggtcctcggt gggggtgtcg gcggtcaggc
     8881 cgatggcgct gttggtgtag cgttcgcctt gcgggccgaa agtatgcagg ggcacgccca
     8941 tgatgagggc ggcgtgctgg cggcccgcca cggcgcgcca ggcgtcccag acccgcgggg
     9001 ggatgtcgtc ctggaagatc ggcagcacgg tctcgggcag gatgatcacg tcgggcgcgg
     9061 gctcgccgtc ggccggcggg cgcagggcca ggtcgaaatg gagcgcgaag ccttgccgca
     9121 gcatcgccgg atcgaatttc tgcgattggg ggatattgcc ctggaccatc cgcacgtgca
     9181 gggggcggcc cacgggctgc gaccaggcga tgccgcgcag gtgccaaccc agcagcacca
     9241 gcaccagggg cagggccagg gccagcaggg cgccgcgcgt gggcttgccg cgccgaggcc
     9301 agaggccggc cagcgcggcg gcggcgcaag cggccaccag ggccatgccg tgcacgccca
     9361 ggaccggcgc ccagccggcg atggggctgt cgacctgggc atagccgatg ttcagccagg
     9421 gaaagccggt gagcacggtg gcgcgcagcc attcgagcag cgtccaggcg gcggcccaga
     9481 tcaatgcggc gcgccagggc gttcgcgccg ggtcgggcag cagccggcgg gccagggcgc
     9541 aggccgcgcc ggggaacagg gcgagaaagg ccgacagcgt cagcacgccg gccgcggcca
     9601 gcggcgcgga caggtcgccg tagtcgtgca tgctgatgta cagccagtac aggccgaggc
     9661 tgaagtacac ggtggcgaac caccagccct gcagcaaggc ctgccggggg cgggcgctgt
     9721 gcagggtggc ccaggcgagc acggccaggg ccagtacctg cgccagggcc agcacgccgt
     9781 cgggcagcgg gccgggggcg aaggtgaggg cgtgcagcgc gcccgccagc gtcaggaggg
     9841 ccggccggcc gatgcgctgg cgcagcggag tgctcattcg gtggctgtgc tggccagggc
     9901 ggaacgcggt tgcggcaggc ggcgggcgcg cagccacagg gcgcgccgcg cgtcggcgcg
     9961 gatgacctcg aggcgcaggt cgccgtaggc ggcgttgtcg ccgcgcttgg gaatgcggcc
    10021 gagctggccg cccagccagc cgccgacgct gtcgtactcg tcgtcgggca gggtggtgtg
    10081 gaagacttcg ttgaagtggc tgatctgcgt cgaggccatc aggcgccact ggccctcgcc
    10141 ttcggggaag atcgattctt cttcgtcttc gtcgaattcg tcctcgatgt cgccgacgat
    10201 ctgctcgagc acgtcttcca tggtgaccag gcccgagatg ccgccatgct cgtcgatgac
    10261 gatggcctgg tgggtgctgc cttcctggaa ctcatgcagc agcacgttca ggcgcttggc
    10321 ttcggggatg aacaccgccg ggcgcagcag cgcgcggatg tccaggccgg gctcgagcat
    10381 gcagcgcagc agttccttgg ccagcaggat gccgatgatg ttgtcgcggt cgtgctcgta
    10441 gacggggaag cgcgagtggg cggcttcgat gatggtggcc agcaactgcg gcaggggctg
    10501 cgtgatgtcg agcaggtcca tgcgcgagcg cggcaccatg atgtcggcca cggtgcgttc
    10561 ggacaccgcc agggcaccct tgatcatggt gtaggcttcg gcgtcgagca gttggcgctc
    10621 gtgcgccgca tcaaggaagg ccttgatgcc ttcgcggtct tcgggctcgc gccgtacgag
    10681 ggacaggacg cgatcgagca gggatctacc ggaggatttt gcgggacgcg tcgttgcgtc
    10741 ggcagcaggg tatgggtctg acatcgtccg ggcggacaag ttttgaagcc gccagcataa
    10801 ccgaatatgg ctgccagtcc gcgattttgt caggtaatcg tgtacggatc ggcgatgttc
    10861 atacgggcaa gcacctggat ttccagggtc tccatgcgtt gcgcgtcgcg tttgttcaga
    10921 tggtcgtaac ccagcgagtg cagcacgccg tggatggtga ggtgcgcggc atggtgcagg
    10981 aagggctttt tctgctcgcg ggcttcgcgg gccagcaccg gggcgcagat gatgatgtcg
    11041 ccgtgcgcgg tgcccttggg gtcgacgccg tattcgaagg tgagcacgtt ggtggcgtag
    11101 tcgcgcccgc ggaaatcgcg gttgagcttg cggccttcgg ccaggccgac caggcgcagg
    11161 ttgagttcgg cgcggccgaa gacggcctgg ccgtcttcgg ccgcggcctg cagggcgtag
    11221 cccgcccagt tgcgcaggcg ccagcggggc aggcgcggct cggcggggcc gtattggacg
    11281 gtgagggaaa gggccgcccc aaggccggcg cgtccttcgc gggggacggc aagcgcggcg
    11341 cgtgcggcat cggagcgctt actgcctgtc ttcatcggcg gaggccttgt cgtaggcgtc
    11401 cacgatgcgc gccaccagcg ggtggcgcac cacgtcgcgg ctggtgaagc gggtgcaggc
    11461 aatgccctgc acgtcttcca gtacctgcac ggcatgcgcc aggccgctct gctggccgcg
    11521 cggcaggtcg acctgcgacg ggtcgccggt gatcaccgcc ttgctgccga agccgatgcg
    11581 ggtgaggaac atcttcatct gctcgggcgt ggtgttctgc gcttcgtcca ggatcacgaa
    11641 ggcgtggttg agcgtgcgcc cgcgcatata ggccagcggc gcgatctcga tggtctgctt
    11701 ctcgaacagt ttctgcacgc ggtcgaagcc catcaggtcg tagagcgcgt cgtacagcgg
    11761 gcgcaggtag gggtcgacct tctgcgccag gtcgccgggc agaaagccca ggcgctcgcc
    11821 ggcctcgacc gccgggcggg tgagcaccag gcgctgcacg gtctcgcgtt ccatggcgtc
    11881 gatggcgcag gccaccgcca gccaggtctt gccggtgccg gccgggccga cgccgaaggt
    11941 gatgtcgtgc ttgaggatgt tgttgaggta gtcgcgctgg cgcggcgtgc gcgggcgcag
    12001 gtcgctgcgg cgggtgcgca gcgggatgct gtcctcgtcc gcgagggacg gcaggccgtt
    12061 gtcgtcgagc gtgggcgcct cggtgggaat ggcgggcgcc tcggcggggc gcacgatgcc
    12121 gatctcgacc aggccgagct ggatgtcgtc gatcgacagg gccttgtgcg tggcctggtc
    12181 gaagaagcgg cgcagggcgc ggccggcgag ttcggcctgg ccgccttcca gcgtgatgcg
    12241 gctgccgcgg cgcgacaggg tgatgcccat gccgtcggcc agctggcgca ggttctcgtc
    12301 gagcggcccg caaaggttgg ccaggtgggt gttgtcgcca tcgaggttga cgatgaccgg
    12361 catggagcgg gcgcccttgg cgccgcgcgc cgtgccccgg gggcggcccg ccgcgcccgc
    12421 gctggttttg gcgtgcgcgg tgctcatcgc gccgcgtcca cgtcggccac gcgcgcgcgc
    12481 agcgagttgg ggtaggcctg cgtgatgacc acgtcgacca tctggccgat cagccggggg
    12541 tgggtggatt ccgggctggc aaaattgacg atgcggttgt tctcggtgcg gcccatgagt
    12601 tccttgtcgt cgcggcgcga cgtgccttcc accagaatgc gctggcgcgt gccgaccatc
    12661 ccctgggcga tggcggcggc ctgttcgttg atgcgtgcct gcaggcgctg caggcggcgc
    12721 agcttgatgt cttgcggggt gtcgtcgtgc aggtcggccg cgggcgtgcc cggccggcgc
    12781 gaatagacga acgagaagga cgtgtcgaag cccacgtcct cgatgagctt catggtcttt
    12841 tcgaagtctt cctcggtttc gccgggaaag cccacgatga agtccgacga cagcgtgagc
    12901 cctgggcgcg cggcgcgcag cttgcgcacc accgacttga actccagcgc ggtgtagccg
    12961 cgcttcatcg cggccagcac gcggtcgctg ccggcctgca ccggcaggtg caggaaggac
    13021 accagcttgg gcaggcgggc ataggcctcg accatgcgct gggtcatttc cttgggatgc
    13081 gaggtcgtat agcgtatgcg ctcgatgccg gggatctcgt gcacgtattc gagcagggtg
    13141 gcgaagtcgg cgatctcgcc ggtatcagac atcttgccgc ggtaggcatt gacgttctgg
    13201 cccagcaggg tgacttcgcg cacgccctgg tcggccaggt cggccacctc gaccagcacg
    13261 tcgtcgaagg ggcgcgagac ttcctcgccg cgggtgtagg gcaccacgca gaagctgcag
    13321 tacttgctgc agccttccat gatggagacg aaagccgtgg cgccctcgac gcgcggcggc
    13381 ggcatattgt cgaacttctc gatctcggga aagctgatgt ccacttgcga ggcgcccagg
    13441 gcgcggcgct tgtcgatcag ctcgggcagg cggtgcaggg tctgcgggcc gaacaccacg
    13501 tccacatagg gcgcgcggcg gacgatggcc tcgccttcct ggctggccac gcagccgccc
    13561 acgccgatca ccagattggg attggccttc ttcaactggt tggcgcggcc gaggtcggag
    13621 aacactttct cctgggcctt ctcgcgcacc gagcaggtgt tgaggaggat gacgtcggcg
    13681 tcttcgggcg tctgggtggg ttcgaggccg tgctccgcgc gcagcacgtc gagcatcttg
    13741 tccgagtcgt actcgttcat ctggcagccg aaggtgcgga tgtagacctt gcggggcgcg
    13801 ccgcccgtgg cggtggctgc ggtggagacg atgggctcgc cgcttttgcc ctggatggac
    13861 ttggtcttgc cgccggattt gcttccgggg cgggagaggg tggtttcttg catgatgtgg
    13921 cgccgtgacg ggtgttgatc ccgggggcgg agagagcggt aaacgaagga ttttaccgcc
    13981 gatgcaaaga aagggggtca gtcgcccaga atcgcggtga aatcacactg aatcaggcgc
    14041 ttgccttcca gggctgcctg catcgtgtgg cgcgctggcc gggtgtcggc gtctggaaag
    14101 accgagagcc atacctcatt caatgccgcg cgcgccttgc tgtctttcat ccagacggtc
    14161 atctttatga tgtcgtccat cgaacctccg gcggtgcgta cgatgatgtc gacctgctgg
    14221 aacaagaaca cgcactgctg ttccaatgtg tcgggaaatt cgcccgtggc tggatcgacg
    14281 ccgttgatga cgcccgtggt cacgatgttg ccgatgcggc aagctgcggg gatgggattg
    14341 ttgtgcgcga atcccttgac gtaaatggct tggcggcggg tcatggcagt ctcctcgtat
    14401 cagtaaacgc tgctcgggcc gttggcctct ggttcggggg catgcttgaa gctgctggcc
    14461 agccgtcgcg ccgagttgac gagcaaggtg gtgtccacgc ccacggccac gaacagcgcg
    14521 ccggcatcca ggtatgcgcg cgccagcttg gcgtcggaag tcagaatgcc gggggccttg
    14581 ccggctgccc gcacttgctt gatgccttcc atgatggcgt cttgcacctc aggatgcacg
    14641 ggattgccca gatggccgag cgaagccgac aggtccgccg gaccgaagaa tacgccgtcc
    14701 acgcctggcg tgcgggcgat ggcttccagg ttgcgcaggc cggccgcgct ttcgatctgc
    14761 accagcaggc acatctcggc gtcggcgcgg tgcagatagt cggggatctg gttccagcgc
    14821 gaggagcgcg ccagcgcgct gcccacgccg cgtatgccac tgggcggata gcgggtggcg
    14881 gccaccatct gcgccgcctg ttcggcggtc tcgaccatgg ggatcagcag tgtctgggcg
    14941 ccgacgtcga gcacctgctt gatcagcgcc acgtcgccga tgggcgggcg cactaccggg
    15001 tgcgaggggt agggtgccac ggcctgcaat tgcgccagcg tgctgcgcag atcgttgggc
    15061 gcgtgttcgc cgtcgatcaa cagccagtcg aagccggtga tggccagcaa ctcggcgacg
    15121 taggcatcgg ccaggccgac ccataggccg atctgcgcct tgttttcact cagggcttgc
    15181 ttgaagagat tgcgggagat ttccatatga gttctgccgt tgctagatga agcggaatga
    15241 cacggagccc agcgggccat agtcgacgtg gaaggtgtct ccaggctgcg ccggcgtggg
    15301 ccgtgtgaac gagccagcca acacgatatc tccggcgttg agctgctcgt cgtggcgggc
    15361 gatcttgttg gccagccagg cgatgccgtt ggccggatga ttcagcacgg cggccgccag
    15421 gccggactcc tcgatgacgc cgttgcgatg cagcagggcg ccgacccatc gcaggtcgat
    15481 actgtcgggc ttgacggggc gaccgcccag caccacgccc gcgttggcgg cgaagtcgga
    15541 gatcgtgtcg aatactttgc gtggcgcctt ggtgtcccgg tcgaactgct cgatgcggga
    15601 atcgatgatc tcaatggcgg gcacgacata ctcggcggca gccagcactt ggtagagcgt
    15661 gacgccgggg ccgcgcagcg gttcgcgcag caagaaagcc agttcgacct cgacgcgcgg
    15721 tgcgataaag cgcgaatagg ggatgtcgcc gccggtctcg aagaacatgt catccatcag
    15781 cggagcatag tcaggctcgt tgatctgcga cgataactgc atggcgcgcg aagtcaggcc
    15841 gatcttgcgg ccccggatgg tgcggccatc gacctgctcc aaccgcaccc attcggcctg
    15901 gatccggtac ccgtcgtcga tcgtcatgtc tgggtgcgcg gtcgagaaat gacgcaattg
    15961 ggtgcgcgtc ttgcgggcct ggtacaactg ctgggccagc gcagagatgg tgtcgggaga
    16021 aagcatatgc aaggtctttt cggctaggga gaaaagtccg gcccgttgcg cgcgcgggcc
    16081 ggctggattc agtcgggcag gtcgtcggcg gcgatggtgt tgactaggcg gcctatgccc
    16141 tcgacctcgg tgatgatctc atcgcctacg gcggcgtcgg acacgccgtc gggcgtgcct
    16201 gtcaggatga catcacccgg gttgagcgtc atgaagctgg acaagtaagc caccagcgcg
    16261 gggatgtcga acaccatgtc tcgcgtattg ccgtcctgag tcggatggcc attgacccag
    16321 ctgcgcagcc gcaggttcat gggatcgggc acgtcggcgg catcgaccag ccacggaccg
    16381 atgggcgtgg tggcatcgcg gcttttgacg cgtagattgg ggcggtagta gttttcgagg
    16441 taatcgcgga tggcgtagtc gttggccacg gtatagccgc cgatgacctc gtaggcgcgt
    16501 tctctgggca cgttcttgca gcgcgtcccg atgaccacgg ccagctcgca ctcatagtgc
    16561 atgtaattca cgtcggcagg ccggcgcgtc gtggtgcgat gtccggtgaa cgatccgggg
    16621 cctttgagga acacgaccgg cgcatcgggc gccttgttga aggcgatttc cttggcgtgg
    16681 tcggcgtagt tcagtgccag aacgaagatc gtgccgaatt ccatgggcgg cagccaggaa
    16741 acctcgagtt cgtccagcac gcggccgtcg ctgagccgga ctttgccgcc gccggcatcg
    16801 gtggcctcgt aggtctggct ttggtagatg acgcgtgcgc gcttcatgcg gcggctcctt
    16861 cggcgatcag ctcgtgctcg aggcttccca gtccggccac ctcaatgcga atgcggtcgc
    16921 cggggcgggc gagcggcgcg ccctcgggcg tgccgaccag cagcacgtcg ccggcggcca
    16981 gcgtcatgtc ggcgctgacg tcggcgatga gttgggcaat cggccggcac aggtcttgca
    17041 ggctgcggcg gtgcgccaga tggccgttga tggcgatatc gatcacggcc ttgccgggtt
    17101 cgaaatccgg gccggcgtgc agcggcggcg tcatagggca gaatccgtcg cggcagcgct
    17161 gtagcagcgg cggccggtag agatcgtcgt gcggcagcgt gatgtcgctg gcgatgagat
    17221 agccgccgac atagtccatt gcattgctcg cctcgacgcg ccgcgcatcg tgccgcatga
    17281 ccacgccgag cgtggcatcg atgcgtacca cgccaggatc ggccggaacc tcgattcgag
    17341 ccccgggcgg ggcgtaggta ttggccggct tgatgtagag caccggatgg actggcggtg
    17401 ccttgtatgg cggttgtgtg aatgcgttgg ccatgcgcgc gagcgtggcc cggtcgttta
    17461 gcagtgtgcc gtagacgata cgaggaagga aagtgcgatt cgccatgctc gcccttgtga
    17521 ggtgcgacgg cagcgatcgc cgccgcgctt tgatcagcgg atagtttcgt cgttaattat
    17581 ttaacaggtt aagtatatag atggcatgca acccaggcaa gcttcaggcg ggcgttgtac
    17641 gacgtgccgc ttgcagcctc tgggcctgga aagcaccgtc catactagaa tgcccaggtg
    17701 aaaaaaaaat ccagatcaaa tccggccgtg gcgactgatg agatgcgcga aggcttgcgc
    17761 gctttttccc gctcgctgcc catggcgctg ctgcgcgccc gcgaatccgt catggggcgc
    17821 tttcggccca tgctgcgcga tcacgacctg accgaacagc agtggcgcgt gttgcgcgcg
    17881 ctcaatgccg ccaaggcgcc gctgagcgcc aacgagctat ccgaccagac gttcatcagc
    17941 atgccgagcc tgtcgcggct tttgaggacc ttggaagccc gcggtgtcat cgaacgcacc
    18001 gcgcaccacg aggacttgcg ggtggcgcag ttgtcgatca accgcgtcgg ccgcgagctg
    18061 gtggctgtgg tcgggccgct gtcggaagcc tcatacggcg agatcaccga gcagatcggc
    18121 gaacacgaac tggaacagct cgagcggctc cttaatcatg tggtggcgcg gctcggattg
    18181 ccgcgttgac gccggtcgcc ggcgggttcg ctgctatacc ttgttggtca ggccttgcgc
    18241 ggccgggtat cggtcgaaga agctttcgtc gacatcgatg cccaggcctg gcctgtcgtt
    18301 cggcagcacg cagccgtcgg cctcgacgcg gtagggcgtg gtgaccaatt cgtcgcggaa
    18361 cttgttgccc tcggagacgt cggcttcgaa atagccggga ttttcgatgc tggcgaggaa
    18421 atggatcgac gcggcctggt tgatgccggt catcgaactg tgcacatgca ccggcaattt
    18481 gtgggcggcc gccagcgccg cgatgcgcag gcattcggtg atgccgccgc tcttggacag
    18541 atcgggctgc agaacctgca cgctgccttc ttcgatcagg cgcgcgaaat cgactttgcc
    18601 atatagattc tcgcccgcgg ccagcggtgt gcgggcattg cgcgcggcct gtcgatatgc
    18661 ctcccgatgg tccatcggaa agggttcttc gagccaacct atgcccagtt cgtccatgac
    18721 cggcagtacg cgctgcgccg ccgccaggga ataggcggta ttggcgtcgg tcatgacggt
    18781 cagcgcgggg aaggcggcgc ggatcgcgcg caggcgttcg atatcccgtt cgggcgtatc
    18841 gccgacccgc agcttgaccg cgctgtagcc ggcgtcggca agtgcccgta cccgctcgat
    18901 cagttttgcc ggctcctcga atcccagcga gagcccgccg gcgtacgccg gcacgcgccg
    18961 ccggctgccg cccagaagct ggtacagcgg catgccggcc accttgccgc gaatatccca
    19021 gagtgcaagg tcgatgccgc ttagcgcgat atagcaggcc gccccggcgc cgtagccggc
    19081 caggtggtag ttgtagacct tgctccatac gtccaccgcc gcggtggcgt ccatcccctg
    19141 caagagggga tggaggtagg cattgaccag cgtggcgatg gccatgtggc tgcgcgcgtg
    19201 gaagcattcg ccccagccca ccaggccgct ggcggtgacg accttgacga ccacggcatc
    19261 ccgcctgacg acacgcccgg tacccatgcc gggctgggca tggggcggca gcgggtacga
    19321 cacgggaaag gcgtggattt ccgcgatgga atgggattgc atggctctat ccgtatttag
    19381 tcgacggtcg cgcccgatgc cttcaccaga cccccccagc gcttttcctg cactgccaga
    19441 aacgcgccga attcggggcc ttgcgccgtg gcggtttcgc cgccctgcct ggagacaaag
    19501 tcgcggaaat cgggcgagcc gacgatctcg gttatgttct tggccagtat gtcgaccact
    19561 tcggccgggg tgccggccgg ggccacgatg ccgtaccagt tcttcaggtc gatatcggca
    19621 tagccggctt ctttgaatgt ggggatctcg ggcgcgacag gcgagcggtc gggcgagatc
    19681 acggccagtg cgcgcagctt gcccgagcgg atgtaaggca gcgacgactg cagcgtgtcg
    19741 acggcgaagg cgacgtctcc cgtcatcgtc gccgtgacca tggcggggct gccggcatag
    19801 ggaatgtgca cggcatcgac gccggcaagc tttttgaatt gttcggccag cagatggctg
    19861 gtggagccgt tgccgaacga accgtacgac agggcgccgg gcttggactt tgcgtaggcc
    19921 accagttcgg ccaccgtctt ggcgggcacg gacgggttga ccaccagcac atgggtgttt
    19981 tgcgacacca gcgcgaccgg cgtgaaatcc ttgatgggat cgtagggcag tttcttcttg
    20041 tagagggacg gcccgatcga aaacgcgccg gttgaggcaa aggccagggt gtagccgtcg
    20101 ggcgccgact tggccacgaa attgcccgcg atcaggccgc cggcgccagt gcggttttcc
    20161 acgaccacgt tgccgtgcag tttttcggac agacggctgg cgacggtgcg cgccaacaca
    20221 tcggtggcgc cgccgggggc gaagcccacg accaggcgga tcggtttgcc gggataggaa
    20281 tcggctgcat gggcgcaggc gccgaagagc gcggccgtgg acaccgctgc cgcgatgatt
    20341 ttggtcagga ttttcatatt gaatcagtcc aaggatcagg gttgcgtcac ggaaaaagct
    20401 tgcagaatct gcgggtcggg atcgatgccc aggcccgggc cggtcggcac gcgcaagcgg
    20461 ccgttgacga cttcaatggc atcgccgtat gggtgcgcct cgaaagtgca gaacagtttt
    20521 tcgagcggcg actcttgttc cagcgctgcc gccagttgca atgtggcgaa caggccaggg
    20581 ccgaaatagg gcgagtgggc gatgggctgg gcgcgggcgc tgccctgggt tttgctcagc
    20641 ggcgtcaacg cttggtaggc agcgcgcatt tctgatatgc cgccgatctt gatgaccgat
    20701 ggctgtacga cgtcgagcag gccgctgcgc gacatggctg ccagttccca cggcgtggcg
    20761 gcgttttcgc cggcggccag agggatgtcc gcgcgttcgc gcaggacacg gaaattgtcc
    20821 agattctcgg gcggccaggt gggctcttcg agccacagca ggttcatcgc ctgcagtgcc
    20881 ggcaaatagg agcaggcctg ctccagtgtc caggcacaat tggtgtcgac catcagcccg
    20941 ggacccgcgc ccaggctgtc gcgcgctatc tgggccaggc gcaccgtgct ttcatggatc
    21001 ttgaccgcgg agtagcccag gtccttggcg tgcgcgcaat tgcgcgccag cgcatcggcc
    21061 tcgccgtact tcatcaggct ggcgtaggcc ggcagcgagt cgcgtcgcgc gccgccgaat
    21121 agctcggcca gcggcttgcc ggccttcttg ccggcgatat cccacagggc gatgtccagg
    21181 ccggagagcg catagttgac ggggccggtg cggccgaaca catggaagcg gcggcgcagc
    21241 tcggtcatca gcgcatcgat atcgttttcg tcgcggccga tgatgaaggg cgcaatgaaa
    21301 tcgtccatgg ccttgcgtat cacgggccac agattgaagc cgaacgcctc gccccagccg
    21361 gtatagccgt cgctggtctc gaccttgaga tacagcgcct cgatgtggct gcgaggttgg
    21421 ccaaggaatt cctgcttggg gccgtcgaca tcgaaaggga tgaggacggg gatggtatga
    21481 atagcggcaa ttgccatggt gcgaagctcc ttgttcagtc gacctggatg ttggcctgct
    21541 tgaccaacgt ggcccattta tcgagttggg aatcgatcca cgctgtgaac tcctgaggcg
    21601 tgtcgccgat gggttccagg cccagctcgt cgaaatggcg cttgagttcc gggtctttca
    21661 gcgcctggat cacggcctga ttgaccttgt tgacgatggc gtcgggcgtg ccggccggcg
    21721 cgacgaggcc attggtggtg accatgtcga cgccgggata acccgcttcg gcgaaggtca
    21781 tgacgccggg cattgcgggc gagcggtgcg ggccggtgat ggccagcgcg cgcagattgc
    21841 ccgcggcgac ttgctggcgc gtggtgtgcg ggccgccgaa caccatggaa acctggccgc
    21901 tgatgacatc ggtcatggcg gggcccaagc cgcgataggg cacgtgcagg atgtcgatgc
    21961 cggccgagac cttgagttgt tcgccggcga tatggatggc ggtgccgggc ccggcgctgg
    22021 cgaacgaata cttgccgggc gattgcttgg ccagcgtgac gagctctttg attgtcctgg
    22081 cggggatttg cgggttgatg gtgagcacct gcggcgaggc ggccatcaag accacgggct
    22141 tgaaatcttt gcgtgtgtcg tagggcaagg ggatcttgct gaggctgggg ctgaccacga
    22201 aggccgagtc tgtgacgcca aaggtggtgc cgtcaggcgt ggcgcgggcc acggcttgca
    22261 gaccgatggt tccgttggcg ccggcgcgat tttcgaccac agcggtagcg cccagcgcct
    22321 tgccgatgta gggcgcgatc aggcgcgcca cggtgtcggt ggagccgccg gggacatacg
    22381 gaacgatcag tctgatcgtc cccggcgcct gctgggcttg ggcggccaat ggcgccgcgg
    22441 cgtaaagcac ggccacggct atgccgagaa gagttcgaaa tgaacgcatg gtttgtctcc
    22501 tgtcatctcc gcccgggctt gccgggcagg tggcggcttt gagtgccgcg cttaataatt
    22561 taacttgtta attaattata tagttaactt gttaattaaa aaatcatagc ggatttttcc
    22621 gctgatttcc aagccagggc cgctgccttg cccgagcctt ggaacgcgtt cttgaattct
    22681 ggaaatgcgg gtcgtcgggg cgtccgctcg cgcggcacct tacttccacc aggcggaata
    22741 acgcctcctg ccagccggtg ccgcgctgga tctcgttgat accattcctg cgcatcgata
    22801 ccgccccggg cgcagcgcgg ccatgccgcg catgcgcaag ggatcagcac acggcctggc
    22861 cagcccccgt gcgaataaca atagacggag acaatcatga cattgcaaag acgtattgcc
    22921 ctcaagctga cccaaggcct gctggcgctg gccgcgtgcc tggcggccac gctggcgcaa
    22981 gccgattatc ccgaccaccc catcactttc atttcggcct acgtgcctgg cggcaccaac
    23041 gactttctca cgcgtttcat cgcgcaaggc gtgggcaagg acctgaattc cagcacgatc
    23101 gttgaaaaca agccgggcgc caacggcatc gtgggaacca ctttcgtgtc ccgctccgcg
    23161 ccggacggct acacgctgct gatgggcaat agcgcgtcgc atggcatcaa ccccacgctt
    23221 tacccgaact tgcaatatga cgcggtcaag ggtttccagg gcgtcagcat ggtggcgtcg
    23281 gtgcccatcg tcctggtcgt caatgccaag ctgccagtgc ataacctgaa ggagctggtg
    23341 gaatacggca agagccaccc cggcaggctc agcttcggca gcagcggcac cggcggcgcc
    23401 ggccatctgg cgggcgaggc gttcatcctg tcttccggca tcgatatggt ccatgtgccg
    23461 tacaagggcg acgccgcggc cgtggcagat accatggcgg gccagatccc ggcctgtttc
    23521 gtggcgctgt cctcggccac tccgcaactg gcctcgggca tgttgaaggt tcttgcggtc
    23581 gccagcgata agcgcctggc caagtatccc gacgtgccca cgatggacga atccggctat
    23641 ccgaacatgg agttcgccca atggttcgcc atcgtcgcgc ccgctgccac gcccaggccg
    23701 gtcatcgaaa aattgaacct gtccatcaag cgggtgctgg agtcgcccgc ggccaggagc
    23761 agcttcgaca cgcagggcgc cgagcctgtc tacaccacgc ccgagcaaac caaggcgttc
    23821 atcgaatcgg agatccagcg ctttggctcc atcatccaca aactgaacct gcatgctaac
    23881 taacttcgat ccccatggca gcacaaccat gaacgcgaaa gtcgcaaaga tagagacctt
    23941 gagttgcgat gcgggctggc gcaattacca cttcgtgaag ctcaccacgg aagacggcat
    24001 cgtcggctgg agcgagttcg acgagcggcg cggcgtgccc gcggtggccg ccatcatcga
    24061 gcaattgggg caagggctga tcggcgcgcc ggtcggggcg catgagcgct tccatgcgca
    24121 ggccagcgct gccacgcgca ccgctgtgcg cggtgtcgtc tcgcaggcga ttggcgcgat
    24181 cgagaacgcg atgctggatg ccaaggcgcg cacgctgggc gtgccttgct acgaactcct
    24241 gggcggcaag atccgcgacc ggctgcgggt ctactggtcg cattgcccga cctggcgcat
    24301 tgcccacccg caatactatt ccccggcggt caccgacctg gacggcgtca aggccgcggc
    24361 gcgcgaggcg cgcgagcgcg ggttcggcgc gctgaagacc aatatgttca tccacgatga
    24421 gggcccggcc tatttctgga gaccggcgtt cggttcgccg ttccagccag aggtcaatgt
    24481 cgaccgcaag gtgctgcgca atgtgcgtgg ccatttggag gccctgcgcg atggggccgg
    24541 tccggagatg gacatcctgc tggatctcaa cttcaatgcc cggaccgaag ggcacctgaa
    24601 gcttctgcgc gcgattgcgg acctggacat attctgggtg gagatcgatt gtttcgagcc
    24661 gaatgcgctg gcgtacattc gcgatcacag cccgcatccg atcagctccg gtgagacatt
    24721 gttcggcgtg cgcgacttcc tgcctttctt ccgccaggac gccatggacg tggcgatcat
    24781 cgacgcggtc tggaacggtg tatggcagtc gatgaagatc gcggcgctgg ccgatggctg
    24841 cgagatcaat atcgcgcctc acaatttcta tggccacttg tgtacgatga tgaacgccca
    24901 tttcgccgct gcggtgccca atctgcgcat catggaggcc gacatcgacc ggctggcctg
    24961 ggacgacgaa ctctatacgc acgcgcccga gtaccacgat ggccacctca tcattcccga
    25021 tcgtcccggc tggggtactg aaccgaatga ggacgcgctg cgcgcccatc ccgcgcgctc
    25081 ggaaaaatag cgcggcatct gcgcaaggga gcgccaggtg caaagcctg
//
LOCUS       DASWPO010000152         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2087484, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000152 DASWPO010000000
VERSION     DASWPO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087484"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VGC69_08490"
     CDS             complement(<1..1026)
                     /locus_tag="VGC69_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY0295351.1"
                     /translation="MSSPSNTHDHATSAYQAKDVNEAFRGGERVPAFLGQINEASIVM
                     LAQAGILDRATAGVIAAGILSLAQDAPGTQRTGDYLDYEKRLAERIGAQASLVHAGRS
                     RQDIASTLSRMNLRVDLLQAHAALARARLEAIALARQHTDTVIPAYTHGVQAQPTTFA
                     HYLLALSSALGRQLDRLAGAYPRINLSPLGAAALSSSSFAIDRPLLADLLGFDGLVLN
                     TYDANHLAPVDSCLELAGILASCAVQISQFAQDVHSQYLAPLPWLTFKPGDLMGISSL
                     MPQKRNPAALEQLRAQSSLLMSRMQAPFLLAHNVRTGMFDYRAYDPLPSQDSVALFNL
                     LERIIGNL"
     gene            1142..2116
                     /locus_tag="VGC69_08495"
     CDS             1142..2116
                     /locus_tag="VGC69_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295352.1"
                     /translation="MAVTFQQLVCIRELVSNGFSVSKTAEALLNTQPSVSKMIRALEH
                     ELGVDVFARRGNRLVDLTEAGRDVYLLAGRMLRDAGSLRQIKHAAGSGPDVQGALRVG
                     TTHIHARYRLLDVTERFLKQYPQVQIEYVVGTPQDIHAGVRDGSLDIGLSTMPDAVSS
                     GILALKAYEMTRCLVVPAGHPLLSVERITLEELARWPWIVHSESLTSGAVVRRTFQMH
                     GLQPRVVMRAMDVDVMKAYVARGIGIAVLQQMAMEGDENGNLRQIDVDGLFPSSTTMV
                     IVRDDHLLKRFAFDFICMLIPRMRGKAIAAALNMAPPRAGAARGRRAR"
     gene            complement(2138..2986)
                     /locus_tag="VGC69_08500"
     CDS             complement(2138..2986)
                     /locus_tag="VGC69_08500"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY0295353.1"
                     /translation="MDAIFYYDGQWTTENPKLLGPADHAFWMASMVFDGARAFDGLTP
                     DLDLHCQRVIRSARKMLMEPKLTWEEVQKLALEAVARFPAGTALYIKPMFFCADGFLL
                     PEASKTQFVLHVFKVPMPGAEGFSAMSQDTYRRAWSSMAPTDAKASCLYPNGQRATKE
                     ANAAGFDTAVMLDGDGNVAEFASSNLWLAKDGKIATPAPNGTFLNGVTRQRVLALLRA
                     HGASVEERTITPDDLKNADEVFSTGNYGKVIHVNRLDDRKLAYGPAAQLAHKLYMDYA
                     QSAGKR"
BASE COUNT          483 a          931 c         1143 g          551 t
ORIGIN      
        1 cagattgccg atgatgcgtt cgagcagatt gaacagcgcg acgctgtcct gcgagggcag
       61 cggatcgtag gcgcggtagt cgaacatgcc ggtgcggaca ttgtgggcca gcaggaaggg
      121 cgcctgcatc cggctcatca gcaggctgct ctgggcgcgc aattgttcca gcgccgcggg
      181 gttgcgcttc tgcggcatca ggctgctgat gcccatcagg tcgccgggct tgaaggtgag
      241 ccagggcagc ggcgccaggt actgcgaatg cacgtcctgg gcgaactggc tgatctgcac
      301 ggcgcagctt gccaggatgc ccgccagctc caggcagctg tccacgggag cgagatggtt
      361 ggcgtcgtag gtgttgagca ccagtccgtc aaagcccagc agatcggcca ggagcgggcg
      421 gtcgatggcg aagctcgagc tcgacagggc cgcggcgccc aacgggctga ggttgatgcg
      481 agggtaggcg ccggccaggc ggtcgagctg gcggccgagc gcgctggaca gggccagcag
      541 atagtgcgcg aacgtggtgg gctgggcttg cacgccatgc gtgtaggcgg ggatcaccgt
      601 gtcggtatgc tggcgcgcga gcgcgatggc ctccaggcgc gcgcgcgcca gcgcggcgtg
      661 agcctgcagc aggtcgacgc gcaggttcat gcgcgacagc gtggaggcga tgtcctggcg
      721 gctgcggccc gcatggacca gcgaagcctg cgcgccgatc cgttcggcca gccgcttttc
      781 atagtcgaga tagtcgccgg tgcgctgcgt gccgggcgcg tcctgcgcca ggctcaggat
      841 gcccgcggcg atgacgcccg cggtggcccg gtcgagtatg cccgcctgcg ccagcatgac
      901 gatggaggct tcgttgatct gccccaggaa ggcaggcacg cgctcgccgc cgcggaaggc
      961 ttcgttgacg tccttggcct ggtaggcgct cgtggcgtgg tcgtgggtat tgcttgggct
     1021 ggacatgggc ggattgccgg cgtgatgaaa gtgacggcga ctttgcccgc gcctaaacct
     1081 gctgtcaaat gaataaattt gaacttaata ttcactccat ggaatagttt gcggaggcat
     1141 catggcagtg acttttcaac aattggtctg catccgggag ctggtgagca acggcttcag
     1201 cgtgtcgaag acagccgagg cgctgctcaa tacgcagccg agcgtgagca agatgatccg
     1261 ggcgctggag cacgaactcg gcgtcgacgt cttcgccagg cgtggcaacc ggctggtgga
     1321 cctgaccgag gcgggccgcg atgtctacct gctggccggc cggatgctgc gggacgccgg
     1381 ctccttgcgc cagatcaagc acgctgccgg cagcggcccc gacgtgcagg gcgcgctgcg
     1441 ggtgggaacc acgcacatcc acgcgcgcta ccgattgctg gacgtgaccg agcgcttcct
     1501 caagcagtat ccgcaggtcc agatcgaata tgtggtgggc acgccgcagg acatccacgc
     1561 gggcgtgcgc gacggcagcc tcgatatcgg gctaagcacc atgcccgacg ccgtgtcttc
     1621 cggcatcctg gcgctcaagg cctacgagat gacgcgctgc ctggtggtgc cggccgggca
     1681 tccgctgttg tccgtcgaac ggatcacgct ggaagaactg gcgcgctggc cgtggatcgt
     1741 ccatagcgag agcctcacct cgggcgcggt cgtgcggcgc accttccaga tgcacggctt
     1801 gcagcccagg gtggtgatgc gcgcgatgga cgtcgatgtg atgaaggcct atgtcgcgcg
     1861 cggcataggc atcgcggtgc tgcagcaaat ggcgatggaa ggggatgaga atggaaatct
     1921 caggcagatc gacgtcgatg gcttgtttcc gtcgtcgacc accatggtca tcgtgcggga
     1981 cgaccacctg ctcaagcgtt tcgccttcga tttcatctgc atgctgatac cgcgcatgcg
     2041 gggcaaggcg atcgccgccg cgctgaacat ggcgccgccg cgcgcgggcg cggcacgcgg
     2101 gcggcgagcc cggtagccgg cgcctttgcc gcgggcttca gcgcttgccc gcggactgcg
     2161 cgtagtccat gtacagcttg tgcgccagct gcgcggccgg gccgtaggcc agcttgcggt
     2221 cgtccaggcg gttgacgtgg atcaccttgc cgtagttgcc ggtggagaac acttcgtcgg
     2281 catttttcag gtcgtcgggc gtgatcgtcc gttcttccac gctggcgccg tgggcgcgca
     2341 gcagggccag cacgcgctgg cgcgtcacgc cgttcaggaa ggtgccgttg ggcgccgggg
     2401 tggcgatctt gccgtccttg gcgagccaca ggttgctgct ggcgaattcg gccacgttgc
     2461 cgtcgccgtc gagcatgacg gcggtgtcga agccggcggc attggcttcc ttggtggcgc
     2521 gctggccgtt cgggtacagg caggaggcct tcgcgtccgt cggggccatg ctggaccagg
     2581 cgcggcggta ggtgtcctgc gacatggccg agaagccttc ggcgccgggc atgggcacct
     2641 tgaacacgtg cagcacgaac tgggtcttgc tggcttcggg cagcaggaag ccgtcggcgc
     2701 agaagaacat gggcttgatg tagagcgcgg tgccggccgg gaaccgggcc acggcttcga
     2761 gggccagttt ctggacttct tcccaggtga gcttgggctc catgagcatt ttccgggccg
     2821 agcggatcac gcgctggcag tgcaggtcga ggtcgggcgt caggccgtcg aaggcgcgcg
     2881 cgccgtcgaa gaccatgctg gccatccaga aggcgtggtc ggcggggccc agcaatttgg
     2941 ggttctcggt cgtccattga ccgtcgtagt agaagatggc atccattctt gagtcctggg
     3001 ggtgtatctg agttcttgag ccgcatggag aaacggggca acacggggcg gcttcgctgc
     3061 ggcgggctcg ggcgcgccat caggcgccct cggcccttcg ggcttccc
//
LOCUS       DASWPO010000153         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1730523, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000153 DASWPO010000000
VERSION     DASWPO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730523"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGC69_08505"
     CDS             <1..484
                     /locus_tag="VGC69_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0295354.1"
                     /translation="GVVWLVGAASGGRDVLQPLSQLSASARLDEARQGLAGNDAPQPG
                     FVAVRSVAELDDALARSGKPAMLDFYADWCVSCREMERYTFSDAAVATRMGRMLLLRA
                     DVTGNTAEDRALLKRFKLFGPPGTVFFAPGGRELATRVIGYQDASRFVASLDSAIAAQ
                     "
     gene            496..1407
                     /locus_tag="VGC69_08510"
     CDS             496..1407
                     /locus_tag="VGC69_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0295355.1"
                     /translation="MPDPFPAISRADFGCVSVFFGAKNGKYPDGNQVLIRGRDTRVAF
                     DTPLVSNHIGPEFDATELVVMGHVHEDHMAGLHRLPHAAVQVHEADLPALQSQAGMAA
                     ACGGTPARMAETLERFRREFFYAPRPDATGYVDGAVWELGRSRVRAMHMPGHTAGHTV
                     LLVEPEGVAFLGDIDLSGFGPFYGEATSNLGDFRRTLARVAEIPAQVWVTYHHRGVYT
                     DREVFLRDLAAFAAKLDAREQRLLQWLRESPQTIAQMVERRLLYPQGHQAPWMVDAET
                     RTIEQHLQELLADGRVRVDEAGVFHLA"
     gene            1531..1731
                     /locus_tag="VGC69_08515"
     CDS             1531..1731
                     /locus_tag="VGC69_08515"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0295356.1"
                     /translation="MEFEWDIAKAERNWHKHGVSFNMAARVFLDPGRIEAFDDRSAYG
                     EDRWATIGCAAPAVLYVVYNGA"
     gene            1770..2087
                     /locus_tag="VGC69_08520"
     CDS             1770..2087
                     /locus_tag="VGC69_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0295357.1"
                     /translation="MKKSADNIVKLTLDPKNPPPLTAAERAELKAVMARPDSAIDYSD
                     APELGDAFWAAADKAVHAKKQITLRIDSDVVDFFKHTGKRYQTRINAVLRAYVDAHKK
                     AHS"
     gene            complement(2114..3127)
                     /gene="hemB"
                     /locus_tag="VGC69_08525"
     CDS             complement(2114..3127)
                     /gene="hemB"
                     /locus_tag="VGC69_08525"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY0295358.1"
                     /translation="MTQHLVSAGYPASRPRRLRRDDFTRRLVRENTLSADDLIYPVFV
                     AEGQGLRQAVPSLPGVARYSLDTLLPVAEECVALGIPVMALFPAIDPALKTPDGIEAA
                     NPDGLIPRVVAELKKRFPELGVLTDVALDPYTSHGQDGLIDEQGYVLNEPTIEVLVKQ
                     ALVQAQSGVDIVAPSDMMDGRIGAIRQALEGGQFIHTRIMAYSAKYASAFYGPFRDAV
                     GSASNLGKSDKQAYQMDPGNTDEALREVAADLAEGADMVMVKPGTPYLDVVRRVKDAF
                     GVPTFAYQVSGEYAMIKAAANNGWLDHDKVMMEALLGFKRAGADGVLTYFAVEAAKLL
                     KHG"
     gene            complement(3124..>3269)
                     /locus_tag="VGC69_08530"
     CDS             complement(3124..>3269)
                     /locus_tag="VGC69_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEY0295359.1"
                     /translation="MRTIQAVRKELADIGQLNAIPFSSTDRIGLPEAGLAIENWISPQ
                     IVP"
BASE COUNT          564 a          994 c         1139 g          572 t
ORIGIN      
        1 cggcgtggtc tggctggtgg gcgcggcaag cggcgggcgc gacgtgctgc agcccttgtc
       61 gcaactgagc gcgtcggcgc gcctggacga ggcgcggcag gggcttgccg ggaatgacgc
      121 gccgcagccc gggttcgtcg ccgtgcgcag cgtggccgaa ctcgatgacg ccctggctcg
      181 cagcggcaag cccgccatgc tggatttcta tgccgactgg tgcgtgtcgt gccgcgaaat
      241 ggagcgctac accttcagcg acgcggccgt ggcgacgcgc atgggccgca tgctgctgct
      301 gcgcgcggac gtcacgggca ataccgccga ggaccgcgcg ctgctcaagc gcttcaagct
      361 cttcgggccg ccgggcacgg tcttcttcgc gccgggcggc cgcgagctgg ccacgcgcgt
      421 gatcggctac caggatgcca gccgtttcgt cgcgtcgctg gactcggcga tcgccgcgca
      481 ataggcgtat tgctcatgcc cgatcctttc cccgccatct cccgcgccga tttcggttgc
      541 gtgtcggtgt ttttcggcgc gaagaacggc aagtatccgg acggcaacca ggtgctgatc
      601 cgcggccgcg atacgcgcgt ggccttcgac acgcccctgg tctccaacca tatcgggccg
      661 gaattcgatg caaccgagct ggtggtgatg gggcatgtgc acgaagacca catggccggg
      721 ctgcaccgcc tgccgcatgc cgccgtgcag gtgcacgagg ccgacttgcc ggccttgcaa
      781 agccaggcag gaatggcggc ggcctgcggc ggcacgccgg cccgcatggc cgagacgctg
      841 gaaagattcc ggcgcgagtt tttctatgcg ccgcgccccg atgccacggg ctacgtggat
      901 ggcgcggtct gggagctggg gcggtcgcgg gtgcgggcca tgcacatgcc aggccatacg
      961 gccggccaca cggtgttgct ggtcgagccc gagggcgtgg ccttcctggg cgatatcgac
     1021 ctgagcggct tcggcccttt ctatggcgag gccacgtcca acctggggga tttccgccgc
     1081 acgctggcgc gggtggcgga gattcccgcg caggtgtggg tcacctatca tcaccgcggc
     1141 gtctataccg acagggaggt cttcctgcgc gatctcgcgg ccttcgccgc caagctcgac
     1201 gcgcgcgaac agcgcctgct gcaatggctg cgcgaatcac cgcagaccat tgcgcaaatg
     1261 gtcgagcggc gcctgctgta cccgcagggc catcaagcgc cgtggatggt ggatgccgaa
     1321 acccgcacca tcgagcagca cctgcaggag ttgctggccg atggccgggt gcgggtggat
     1381 gaagcggggg tttttcacct ggcgtaggcg tgtagggtag ggacaaatcc cttcacattc
     1441 cacccaggca ctcgtagcgg atcatgcata ctctgccgcc tgaaagcgga ggccaattca
     1501 tggttcctgt aaactacaaa agtaactaca atggaattcg agtgggacat tgcaaaggca
     1561 gaacgcaatt ggcacaagca cggcgtgagc ttcaacatgg cggcaagggt ttttcttgat
     1621 cctggccgga tagaggcctt tgacgacagg agtgcgtatg gcgaggatag atgggcgacg
     1681 attggctgcg ctgcgccagc ggtgctgtac gtggtctata acggtgcgtg aaggcgacgt
     1741 tatccggctg atttcagcga gaaaagcaaa tgaaaaagag cgcagacaat atcgtcaagc
     1801 tcacgcttga tccgaagaat ccgccgccgt tgaccgccgc ggaacgagcg gaattgaagg
     1861 cggtgatggc caggccggat tcggccatag actacagcga tgcgccagaa ctgggggatg
     1921 cgttctgggc tgccgcagac aaggccgtgc atgcaaagaa gcaaatcacg ctacgcatcg
     1981 attccgatgt ggtggacttc ttcaaacata ccggcaagcg ttatcagacg cgcatcaatg
     2041 cggtcttgcg cgcttacgtg gatgcgcaca aaaaagcgca cagttgatct gaatcaccag
     2101 atggctgcgt gaatcagccg tgcttgagca gcttggccgc ttcgacggcg aaataggtca
     2161 gcacgccgtc ggcgccggcg cgcttgaagc ccagcagggc ttccatcatc accttgtcgt
     2221 gatccagcca gccgttgttg gcggcggcct tgatcatggc gtattcgccg ctcacctggt
     2281 aggcgaaggt cggcacgccg aaggcgtcct tcacgcggcg caccacgtcc aggtagggcg
     2341 tgccgggctt gaccatcacc atgtcggcgc cctcggcgag gtcggccgcc acttcgcgca
     2401 gggcctcgtc ggtattgccc ggatccatct ggtaggcctg cttgtcggac ttgcccaggt
     2461 tgctggccga gcccacggca tcgcggaagg ggccgtagaa ggcgctggcg tacttggccg
     2521 agtaggccat gatacgcgtg tggatgaact gcccgccttc gagcgcctgg cggatggcgc
     2581 cgatgcggcc gtccatcatg tcgctggggg cgacgatgtc cacaccggac tgcgcctgca
     2641 ccagcgcctg cttgaccagt acctcgatgg tgggctcgtt gagcacgtag ccctgctcgt
     2701 cgatcaggcc gtcctggccg tggctggtgt agggatcgag cgccacgtcg gtgagcacgc
     2761 ccagctcggg aaagcgtttc ttgagttcgg ccaccacgcg cgggatgagg ccgtcggggt
     2821 tggcagcctc gatgccgtcg ggcgtcttga gcgcggggtc gatcgccgga aagagcgcca
     2881 tcacggggat gcccagcgcc acgcattcct cggccaccgg cagcagggtg tcgagcgaat
     2941 agcgcgccac gcccggcagc gagggcacgg cctggcgcag gccctggcct tcggccacga
     3001 acacggggta gatcaggtcg tcggccgaca gggtgttttc gcgcaccagg cggcgcgtga
     3061 agtcgtcgcg gcgcaggcgg cgggggcggg aggcgggata gccggcggaa acgagatgct
     3121 gggtcatggc acgatttgag gagaaatcca gttttcgatg gccagcccgg cttctggcag
     3181 gccaatgcga tccgtggagg aaaaagggat ggcgttgagc tggccgatgt cggcgagctc
     3241 cttgcgcacg gcctgtatgg tccgcatgc
//
LOCUS       DASWPO010000154        20853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_391690, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000154 DASWPO010000000
VERSION     DASWPO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20853
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391690"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(553..837)
                     /locus_tag="VGC69_08535"
     CDS             complement(553..837)
                     /locus_tag="VGC69_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295360.1"
                     /translation="MTTCSEVSPVHLVSAIPTTDVAYFIEDLARRCNVTYVPTAMDSW
                     ANTVTCLSGDEVQSGHVQDLLIALRRAGKLSTNEMMSLLVNYLRECQQHR"
     gene            complement(850..1794)
                     /locus_tag="VGC69_08540"
     CDS             complement(850..1794)
                     /locus_tag="VGC69_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY0295361.1"
                     /translation="MPDFLHARKDFDQLLALVADERGLDPMLVEKDYWIMHCLWGLQA
                     QGFQFELKGGTSLSKGFGVIHRFSEDIDIRIEPPADMDVKTGRNQDKPAHVLSRRTYY
                     DELAARIRIPGIGNVERDTQFDDDKMRSAGIRLIYTPRVSALAGVKDGILLELGFDDT
                     APNRAVTISSWAFDVARDRGVDVFDNRAVAVPCYAPTHTFVEKLQTISTKYRRLGESE
                     AFPGNFLRHYYDVYCLLGLEEVQAFMREPAYQERKAQRFRSGDEQVIARNQAFVLADA
                     AQRERFELEYRKTQALYYQGQPDFDALVARIHQYIDVM"
     gene            complement(1787..2398)
                     /locus_tag="VGC69_08545"
     CDS             complement(1787..2398)
                     /locus_tag="VGC69_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295362.1"
                     /translation="MPAATLSAVEPLRGAAAHLADELQAGRVYRREDLARLSTAVDRH
                     LRELVATGKLKKLAQGLYHAPKQSSFGPLPPTDDQVVGGFLRDKDFLVFSPSAYNAVG
                     LGTTQLYNRTLVYNHKRHGVFQLGNRQFDFRMKPRFPKKLTPEFLYVDLLNNLEELAE
                     DRDAVLRQARGKLSSFDSVRLQRAADSFGNVATRKRLREWMSA"
     gene            2636..3469
                     /locus_tag="VGC69_08550"
     CDS             2636..3469
                     /locus_tag="VGC69_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY0295363.1"
                     /translation="MPNAGRQERDTKVGRILLWEGRVSRARLISEYGLSPIRASEWLR
                     DFRESYPEWVAWDSKLKADVATSIAYADAEQAANTSWPGAISAMLVPYALNVPGKPLS
                     ISWDFAHISPRTFSRLNLAIADRLRVRFVYCSMNNPEPHERTVEPHSLLRAGRRWHVR
                     GYCLQRRAFRDFVLGRMSKVTLLTEPSLADIAADAAWSTVLQVRITAHPGLSPAQQLI
                     VRNEYFNGASARVESCRAALLNYMVQEIMAATDVSRQAPPDYQLAIENTEECRQWLFM
                     T"
     gene            3652..4548
                     /locus_tag="VGC69_08555"
     CDS             3652..4548
                     /locus_tag="VGC69_08555"
                     /inference="COORDINATES: protein motif:HMM:NF039513.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetA family
                     protein"
                     /protein_id="HEY0295364.1"
                     /translation="MREITADIAEELQYQDWTPEGDDVTPATPLAIRAIMVFNRLRDA
                     GWIRVDRVGVREMVTMAPAVAHFMNRLVEFAHTGPEFVSGKIRSIEANLKLLLDESTD
                     GASLQEAARQSRALLEHVRIAGTNVRDLMLEIGRVDATGEFVRRFFDDYVERMADGEM
                     VRKKPANPALFKIGPTSTATRTSIAEQARQLQEKIDEFKERYAKLKELLNNLGPFVGG
                     EQDKVHEVLEIFDRITKEAADEEDLTRRLQALNTEEYRQLVQVKPMDSMITEGLDLGP
                     TGFLALFLDSWGDPIVDQPLGG"
     gene            complement(4480..4755)
                     /locus_tag="VGC69_08560"
                     /pseudo
     CDS             complement(4480..4755)
                     /locus_tag="VGC69_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(5343..6935)
                     /gene="guaA"
                     /locus_tag="VGC69_08565"
     CDS             complement(5343..6935)
                     /gene="guaA"
                     /locus_tag="VGC69_08565"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY0295365.1"
                     /translation="MHQRILILDYGSQVTQLIARRVREAGVYSEIHPGDVKDDFIRDQ
                     AKLGLKGVILSGSHASAYDEASMRVPQAVFELGLPVLGICYGMQAMAHQLGGKVTYHD
                     HREFGYAEVRAHGHTKLLEGLQDFATPEGHGMLKVWMSHGDSVDEMPPGFKLMASTPS
                     CPIAGMADESRNFYAVQFHPEVTHTVQGKALLTRFVRDICEAAGDWNMPDYVSEAVAR
                     IREQVGKDEVILGLSGGVDSSVAAALIHKAIGDQLTCVFVDHGLLRLDEGKQVMDTFA
                     KNMGVKVIHVDATAQFMGKLAGVADPEAKRKIIGREFIEVFSAEKAKLKSAKWLAQGT
                     IYPDVVESAGTKTGKAAAIKSHHNVGGLPDDLDFKLLEPLRELFKDEVRELGVNLGLP
                     PEMVYRHPFPGPGLGVRILGEVKREFAELLRRADAIFIEELRAVIDPETGKSWYDLTS
                     QAFAVFLPVKSVGVMGDGRTYEYVVALRAVQTFDFMTADWAPLPHALLARVSSRIINE
                     VRGINRVVYDVSSKPPATIEWE"
     gene            complement(7083..8543)
                     /gene="guaB"
                     /locus_tag="VGC69_08570"
     CDS             complement(7083..8543)
                     /gene="guaB"
                     /locus_tag="VGC69_08570"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0295366.1"
                     /translation="MRLVQQALTFDDVLLVPAYSEVLPRDTSLATRLTRNITLNIPLV
                     SAAMDTVTESRLAIAMAQEGGIGIIHKNLTDDEQAKEVARVKRHEFGIVIDPVTVTPD
                     MKVRDAIALQRQNGISGLPVVEGRKLVGIVTNRDLRFEDRLDQPLRNIMTPRERLITM
                     KEGTTLNEAQALMHKHRLERILIVNDAFELRGLATVKDIVKNTEHPVANKDAQGQLRV
                     GAAVGVGDGTEERIEKLVAAGVDVLVVDTAHGHSRGVLERVRWVKQNFPKVDVIGGNI
                     ATAAAARALVEYGADGVKVGIGPGSICTTRVVAGVGVPQITAISDVARALEGTGVPLI
                     ADGGIRFSGDIAKALSAGAYACMMGGMFAGTDEAPGEIVLFQGRSYKSYRGMGSLGAM
                     TDGSADRYFQDPANNADKFVPEGIEGRVPYKGSVLAIIYQLVGGIRSSMGYCGCATID
                     EMRTKTEFVQITSAGFRESHVHDVQITKEAPNYRAD"
     gene            8823..9692
                     /locus_tag="VGC69_08575"
     CDS             8823..9692
                     /locus_tag="VGC69_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0295367.1"
                     /translation="MSNTHEIRPGQSIELLKALHILTRDGKMNQDSRRKLKQVYHLFQ
                     FIEPLLQDVQDERGAVTLVDHGAGKSYLGFILYDLFFKEKAQARQQGVDAPRSSHIYG
                     IETREELVQHSRELAQHLGFAQGMSFLNLSVADSIASQALPPTVDVVTALHACDTATD
                     DAIRFALEKKARHVVVVPCCQAEVASVLRKNKAHALADPLAEIWRHPLHTREFGSQVT
                     NVLRCLQLEAHGYQVSVTELVGWEHSMKNELIIAQRKQDRPRHKAAERLKEMLERIGL
                     QQLKDRFFAPETA"
     gene            complement(9697..10227)
                     /locus_tag="VGC69_08580"
     CDS             complement(9697..10227)
                     /locus_tag="VGC69_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0295368.1"
                     /translation="MTDTLRVRRVPASQARACVDMLADVLIDCVQGGASVSFMQPLSR
                     EKAVAFWRGVADGVERGERALLVAEDGAGRIVGTVQLVMAQPENQPHRADVAKMLVHR
                     RARRQGIAQRLMTEIEQVAREEGKSVLVLDTVTGGDAERLYARAGWQRVGTVPNYALM
                     PDGPPCGTTFYYKQIL"
     gene            complement(10224..10814)
                     /locus_tag="VGC69_08585"
     CDS             complement(10224..10814)
                     /locus_tag="VGC69_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0295369.1"
                     /translation="MDINSLIAQRVRERRDARGWSLDVLAERSKVSRSAISLIERGQS
                     SPTATVLDKLASALGVAVVSLFEPYAQPAAAPLPVSRAAEQPAWSDPASGYVRRNLSP
                     AWPSPLQLVEVRFPPGQHVAFDTDVRDTGIHQQVWMIEGTMEVTVGEQCWRLETGDCL
                     AMRLDRPIAYHNPARKPARYLVALSGLDFNQARSKP"
     gene            complement(10960..11511)
                     /locus_tag="VGC69_08590"
     CDS             complement(10960..11511)
                     /locus_tag="VGC69_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEY0295370.1"
                     /translation="MIVDDDAARQVAADTLRWVEKAVIGLNLCPFAKAVQIKSQVRYA
                     VSDATDEEGVLADLEDELLLIAEADPEKVDTTLLILPEALHDFYEFNDFGELSDRLLK
                     RLRLVGDLQVATFHPAFQFADTDPGDAGNYTNRSPYPILHILREASIDRAVDAFPEAD
                     AIYEKNIETMEKLGLDGWRKLMD"
     gene            complement(11508..12977)
                     /locus_tag="VGC69_08595"
     CDS             complement(11508..12977)
                     /locus_tag="VGC69_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY0295371.1"
                     /translation="MNARIPDDALPPTLDPDALQAFVNEKWDGEIIPALTTYIEIPAK
                     SPSFDADWEKNAYIERVIRDAAQWVEAQKIHGLVLEIVRLPGRTPVIFFDAPATRADN
                     GDTIMLYGHLDKQPEFSGWRNDLGPWTPKLENDKLYGRGGADDGYAVYASITAIMALD
                     KQGVPRPRCVGIIETCEESGSYDLLPYVDALRGRLGNVALVVCLDSGAGSYDQLWMTT
                     SLRGMVSGTLEVQVLDEGVHSGDSSGVVPSTFRILRHLLDRLEDSGTGRLLPQSLHCE
                     IPAERVEQVKATAKILGEEVWRRFPWSCGADGSFVLPMTAEPEEALLNRTWRPTLSVT
                     GAEGLPPLSSAGNVLRPRTAFKLSLRLPPLIDAVAAAQEIKGLLEDDAPYNAKVIFHA
                     NQGAATGWNAPATAPWLSAALDRASRQYYGAPCGYIGQGGTIPLMSILQQGFPKAQFM
                     VCGVLGPRSNAHGPNEFLHIPYGKKITAAVAQTMADMPA"
     gene            13232..13570
                     /locus_tag="VGC69_08600"
     CDS             13232..13570
                     /locus_tag="VGC69_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295372.1"
                     /translation="MAIRFDTLKIVERLERGGFTREQAKTQAEVLADVINPDALGFAT
                     RQDLQDVRGELRNELHEVEYRLVARITTVEHKLENFQKATEGKFNLLYWMMGFMLTMM
                     SGLFYKLVIL"
     gene            complement(13647..17750)
                     /gene="purL"
                     /locus_tag="VGC69_08605"
                     /gene_synonym="purI"
     CDS             complement(13647..17750)
                     /gene="purL"
                     /locus_tag="VGC69_08605"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY0295373.1"
                     /translation="MSLVQHLPGSSVLSAFRRERLLAQLKQAGLPVADVSARYEHFVL
                     TESELGAQDAQRLADLLDYGTPAAASDAPAKALELLVIPRLGTVSPWASKATDIARNC
                     GLSAVRRIERGIRYTLTPERGLLGSKSFEADTLARAAACLHDRMTETVVDAGFDGQAL
                     FAPLAGKPVRTIPLQAQGRAALVEANAALGLALSEDEIDYLADAFAALRRDPTDVELM
                     MFAQANSEHCRHKIFNAQWKIDGADQPHTLFGMIRATHKAQPNGTVVAYSDNAAVMEG
                     GAAQRFEAGVPGLAGASPVYRRRETTVHTLMKVETHNHPTAIAPFPGASTGAGGEIRD
                     EGATGRGSKPKAGLTGFTVSHLRLPGAEQPWEADHHGLPERIATPLSIMIDGPLGGAA
                     FNNEFGRPNLLGYFRTYEQTTEQTAGEGAQQTRWGYHKPIMIAGGLGSIDASLTHKDA
                     IPPGALLIQLGGPGLRIGMGGGAASSMGVGSNSADLDFNSVQRGNPEIERRAQEVIDR
                     CWQLGRGNPIIAIHDVGAGGLSNAFPELVNDAARGAVFDLKRVPVEESGMSPAEIWSN
                     ESQERYVLSVLPRDLERFEAIARRERCPYAVVGVATEERALRVVDGEGLPGLEGDGTA
                     PRAKPDGEVRPVDVPIDVILGKPPRMTRDVRRLPGAAGKLDLAGIDLTEAAYRVLRHP
                     TVANKTFLITIGDRTVGGLSARDQMVGPWQVPVADCAVTLADYEGFRGEAMSMGERTP
                     IAVIDAPASGRMAVAEALTNLAAADVAQVEDIKLSANWMAACGAPGQDAALYDTVTAV
                     SELCQAVGLSIPVGKDSLSMKTSWTVDGTAGAEVRQVVSPVSLIVTAFAPVKDARASL
                     TPQLRTDIGDSALILIDLGQGRHRMGGSILAQAYGQVGGGDNDGVPDIDRPQDLRAFF
                     ITIRTLAEAGIILAYHDRSDGGLFATLVEMALAGRTGISVNLDMLTFDPNSADWGDYK
                     IRADQVAVQREELTLKALFSEEAGAVIQVPLSQRDAVMQVLRGAGLSAYSHVIGGLNG
                     HDEIEFYRDGKKVWSQPRPELGSAWSEVSHGIAMLRDNPACAQAEFDTWKDVANPGLS
                     PRVAFNPQDDIAAPFIATGRRPRVAILREQGCNSQVEMAWAFDTAGFEAVDVHMTDLL
                     AGRIDLSTMQGLVAVGGFSYGDVLGAGEGWARTIRFNSKLSDQFAEYFARPDTFGLGV
                     CNGCQMMAALAPMIPGAGHWPRFTRNQSEKYEARLSLVEIAESPSLFFAGMAGTRVPV
                     AVAHGEGYADFSQQGNAGKVIGAAHFINHYGNRTEDYPYNPNGSPGGLTSVTTADGRF
                     TVIMPHPERVTRNVMMSWAPEKWGTADTGGQFSPWMRIFRNARVWLK"
     gene            complement(17853..18050)
                     /locus_tag="VGC69_08610"
     CDS             complement(17853..18050)
                     /locus_tag="VGC69_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEY0295374.1"
                     /translation="MTTEFLVPDMSCGHCVKTITAAVQAVAPAAQVKTDLASHRVVID
                     GVDAAQARAAIEEAGYEVQAG"
     gene            18196..20481
                     /locus_tag="VGC69_08615"
     CDS             18196..20481
                     /locus_tag="VGC69_08615"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0295375.1"
                     /translation="MNTLATTELELAVSGMTCASCVRRVEKALAKVPGAREAAVNLAT
                     ERAQVRYDPAEGSPDAFVAAVAKAGYQARPIVAQDNQARELAESRAAEARHLRRDFLI
                     ALALSLPVFILEMGGHLLPAWHHWIAATLGERNGFYLQFVLTSAVLLGPGRRFFSSGL
                     AALWRLAPEMNTLVALGAGAAWLYSSVATFAPHALPDSARQVYFESAAVIVTLILLGR
                     MLEAAARGRTGAAIQRLVSLQPQTARVLRDGQPQDLPIEQLIPDDAVLVRPGERIPAD
                     GTVTEGASYVDESMLTGEPLPVEKTAGAPVTGGTLNTTGSFTLRVSHLGADTVLARII
                     RMVETAQGARLPIQALVDRVTGWFVPAVLAAALLTFAAWMIWGPDPALPHALVNAVAV
                     LIIACPCAMGLATPTSIMVGTGRAAELGVLFRQGDALQALRDVDVVAFDKTGTLTLGK
                     PTMTDWTAPGCDESLLLQRLASVQARSEHPIARAITAAAQARGLAPLPVTGFQAVTGA
                     GVTAQVDGRLLIAGSARLMAELGIDLSAFGNQAADWGRDGKTPIYVAVDGQAQAMAAV
                     ADPLQPSAVQAIAALHAQGLSTAMITGDHRDTAQAVARQLGVDDVRAEVLPEGKVQAI
                     EALRQGGRKVLFVGDGINDAPALAAADIGMAIGSGTDIAIEAAHVVLLGKDLRGVADA
                     IALSRATLRNIRQNLFWAFAYNVALIPLAAGALYPAAGWQLSPMLAAGAMALSSVFVV
                     SNALRLKTFQPARVRPARAGA"
     gene            20499..>20853
                     /gene="cueR"
                     /locus_tag="VGC69_08620"
     CDS             20499..>20853
                     /gene="cueR"
                     /locus_tag="VGC69_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEY0295376.1"
                     /translation="MNIGQAAQATGISAKMIRYYETIGLIGPAGRTAAGYRTYTEQDL
                     HTLRFVRRARGLGFSVEQMHELLALWRDRKRASADVKRVALQHVEELERKAAELQQMA
                     DTLKHLARHCHGDERP"
BASE COUNT         3689 a         6744 c         6782 g         3638 t
ORIGIN      
        1 gacgccatat tgttactcca tttcagagaa aagtcatcaa aaacgattgc agatctgcaa
       61 ttcataacaa aaatgataac aatacgcctc ttcctcctgc ctgccttaga tcccagcccg
      121 gcaaggggcg ctatttgcga caaccattgc tgaagcgcca cggacgccta gagggaggca
      181 actcgcgcac cggcatctcg acacccctag cctcccaggc gctccaccaa accagggaca
      241 ctaaccgcga ctcgccaaac aacaaaccat cgccacccgg tgtcacgtaa gctcgcgaaa
      301 gcacgcttta aggcagttcc ctcagcgtcg cgacatcttg cgggtcaatg cgcagcaccc
      361 gcccgttgct gtacacgagc tccagcacat gagccgttgc caaataccgc gccgacacag
      421 catgaggcgc ctctgacgcc tgatagccac gcgccagcgc ctgttcgtaa ctccgacgag
      481 tgattctggt tgccatgtgc tcgacacctc atcagcaatt tttgcctacc gactcgaaac
      541 gacattgcca gatcatctgt gttgctgaca ttcacgcaga tagttgacta gcagcgacat
      601 catctcgttg gtagaaagct tgcccgcccg cctcagcgca atcagcaaat cctgcacatg
      661 gcctgactgc acctcgtctc ccgaaaggca ggtcactgta tttgcccaac tatccatcgc
      721 ggtggggaca taagtcacat tgcagcgtcg cgcaagatct tcaatgaaat acgctacgtc
      781 cgtggtcggg atagccgaaa cgagatgaac tggcgagact tcactgcatg tagtcatgca
      841 cggcacgtct cacattacgt cgatgtattg atgaatccga gccaccagcg catcaaaatc
      901 tggctgaccc tggtaataca gcgcttgcgt cttgcggtac tccaactcaa agcgctcgcg
      961 ttgtgcagcg tcagcaagga caaaggcctg gtttcgtgca atcacctgct catcaccgct
     1021 ccgaaagcgc tgcgccttgc gctcttggta ggccggttcc cgcatgaatg cctgcacctc
     1081 ctccaaaccc agaaggcagt acacgtcata gtagtgccga aggaaattgc ccggaaacgc
     1141 ctccgactca cccaagcgac ggtatttggt ggaaattgtc tgaagcttct cgacgaaagt
     1201 gtgcgtcggc gcatagcaag gtaccgcgac tgcacggttg tcgaacacgt ctacgccgcg
     1261 atctctggca acatcgaaag cccatgacga gatcgtgaca gctcgattgg gtgccgtatc
     1321 gtcaaaccca agctccaaca aaattccatc tttgacgcct gccaatgccg atacgcgcgg
     1381 cgtgtatatc aagcggatgc cggcgctgcg catcttgtcg tcgtcaaatt gcgtgtcgcg
     1441 ctcaacgttg ccgatgcctg ggatacggat acgcgccgcc agttcgtcgt aatacgtacg
     1501 gcgcgaaagt acgtgcgctg gcttgtcttg attgcgcccc gtcttcacat ccatgtcggc
     1561 gggcggctct atgcgaatgt cgatgtcttc ggaaaaacgg tggatgacac caaagccctt
     1621 cgagagcgac gtccctccct ttagttcgaa ctggaagccc tgtgcctgca aaccccacag
     1681 gcaatgcatg atccagtagt ccttctcgac caacatagga tcaagccctc gttcgtcagc
     1741 gacaagcgca agcaactgat caaagtcctt ccgcgcgtgc aggaaatcag gcactcatcc
     1801 actcccgcag gcgcttgcgc gtcgccacat tgccaaaact gtcggcagcc cgctgcagcc
     1861 ggactgagtc gaacgaggac agcttgccac gcgcttggcg caaaacggca tcacgatcct
     1921 cggccaactc ttcaaggttg tttagcagat cgacatacag aaactcgggg gtcaacttct
     1981 tcgggaagcg cggcttcatt cggaaatcga actgccggtt gccaagttgg aaaacaccat
     2041 ggcgcttgtg gttgtagacc agcgtgcggt tatagagctg agtggtacca agacccacgg
     2101 cgttgtaggc cgagggtgaa aacaccaaaa agtccttgtc acgaagaaag ccccccacca
     2161 cttggtcatc cgttggtggc agcgggccaa agctggactg cttcggcgcg tggtagaggc
     2221 cctgagcaag ttttttcagc tttcctgtgg cgaccagctc acggagatgc ctatccactg
     2281 cggtggacag acgtgccagg tcctcgcgcc gatagacacg accggcttgc aactcatccg
     2341 caagatgagc tgcggcccct cgaaggggct caacggcaga tagagtagct gcaggcacgt
     2401 ctaatttctc attgaaattt catgcatttt atcgcattct gccgactacg tcagtcccaa
     2461 aaaagtgtcc aaagggtcac cgccaatctc tcgatgcgtc ctgtggacgt acccgctgga
     2521 agaggagagg tctctagcgc atgacaataa tcatcgctgg ctacgccaaa tcatatggat
     2581 ttacatatca tatgtttttt cataccatat agacattcat cacaaggcga cacccatgcc
     2641 taacgcgggc cggcaggaaa gagataccaa agttggccga atccttctct gggaggggcg
     2701 ggttagccgc gctcgactta tctccgagta tgggttgagc cctatacggg ccagcgagtg
     2761 gctgcgcgat ttccgggagt catacccgga gtgggtagca tgggacagca agctcaaagc
     2821 agatgtggcc acctccatcg cgtatgcgga cgccgaacag gcggcgaata cctcgtggcc
     2881 gggcgctatc agcgccatgc tggtcccgta tgcgctgaac gtcccaggca agccgctctc
     2941 catctcatgg gacttcgccc acatctcgcc tcgcaccttc tcccgactga acttggccat
     3001 cgcagatcgt ctcagggtga ggttcgtcta ctgctccatg aacaacccgg aaccgcacga
     3061 gcgaaccgtc gagccgcaca gcctgttacg ggcaggacga cggtggcacg tccgcggcta
     3121 ctgtctgcag aggcgagcct tccgtgactt cgtattaggg cgaatgagca aggtcacact
     3181 gctcacggag cccagcttgg cagacatcgc cgccgatgct gcctggtcca cagtcctcca
     3241 agtcaggatt accgctcacc ctggcctcag ccctgcgcag caactgatcg tcagaaacga
     3301 gtacttcaac ggggcatcag cgcgcgtaga aagctgtagg gctgccctcc tgaactacat
     3361 ggtgcaggag atcatggcag ccacggacgt gagtcgacag gctcctcccg actatcaact
     3421 ggcgattgaa aacacagagg agtgccggca atggctgttc atgacttaac agaccaaatt
     3481 cgccaagaga aggtggctga acgctatccg ccgctcttca aacgcctgcc cgaccgcctg
     3541 ttcgcgccgc tggcttcggc caatcgcttc caatattggt cactgctgtg tgttctgcac
     3601 gccaaacgct tcggcccaga ggccccgttg ccgccgagta ccggcttctt gatgcgcgaa
     3661 atcacggccg acatagccga agaactccag taccaggact ggactccgga aggcgatgac
     3721 gtgacgccag ccacaccgct ggcaatccga gccatcatgg tcttcaatcg cttgcgcgac
     3781 gcgggatgga tccgcgtgga ccgagtgggc gtgcgcgaga tggtgacgat ggctcccgcc
     3841 gtcgcccatt tcatgaaccg cctcgtggag ttcgctcaca ccggcccgga gttcgtctcc
     3901 ggcaagatcc gcagcatcga agccaacctg aagctgcttc tggacgagag cacagatggt
     3961 gcctcgctgc aggaagcagc ccgccagtcg cgcgccctac ttgaacacgt ccgcatcgcc
     4021 ggcacgaatg tgcgcgatct aatgctggag atcggtcgcg tcgacgccac cggcgagttc
     4081 gtgagacgct tcttcgatga ctacgtcgag cgcatggctg atggcgagat ggtacgcaag
     4141 aagccggcga atcccgccct cttcaagatc ggcccgacct ctacggcgac tcgcacttcc
     4201 atcgccgaac aagcccgtca gctccaagag aagattgatg aattcaagga gaggtacgcc
     4261 aagctcaagg agctcttgaa caaccttgga ccattcgtgg gtggagagca ggacaaggtt
     4321 cacgaagtcc tcgaaatctt cgatcggata accaaagagg cggccgatga ggaggatctg
     4381 acgcggcgcc tacaagccct caatactgaa gagtatcgac agttggtgca ggtaaagcca
     4441 atggacagca tgatcactga aggtcttgat ctcgggccca cgggctttct cgcgctgttt
     4501 ctggatagct ggggagatcc catcgtggac cagccgcttg gcggctgaca atttgtcccg
     4561 cgcggcagca agcgagatgc caccaaaacc atagcggccg atggtcagcg tttcgcgccg
     4621 gccattgatg ctgtagtcgt atcgaaagga gatggaccct gtcggtgaga tggccacgta
     4681 catcccatca cggtcagcga ctttgtaaag ggtcgagcga ggcttgaggt ttcgaagggt
     4741 gaagtctgta agcatgtaag gtttcctaaa ttgagacggc cggttgccgt caccaatcgc
     4801 gaaacctaca gatttccgct cacactgagc gtccaatcac ttgcttgagc accgaatccc
     4861 gtttcatatg gtctcacgtc ctagccttca acatcgccac ccgaaaacaa ccggcaaact
     4921 ggccgaaaaa cggctctctg ccttttgcca gcttgtatgc aggggcatgg cgccctaagc
     4981 tacttaaaac caaagaacca cgatgaatat ctgaaagaga cgaatgcctt ccagccagac
     5041 gacgtacacc tgcccgccaa cccgcatgaa acctgacttt tttgaccgca gcataccgtg
     5101 tcgcacggta tcggcagacg gtatggcact tagcctagct cggacctaag ctgagagcgc
     5161 catttctacc gtcgccgata ccgtcgattt cagcgtctag caacaatcga ccacgagagg
     5221 atcaagaaga cgattaccct tttaaatcaa tagcttagag gcagctcatc ggctatattt
     5281 catcaacttg cacatgcaaa gcatgccgta agtttttgat ttataaggga ttttttcgcc
     5341 cctcactccc actcgatcgt cgccggcggc ttgctgctca cgtcatacac cacccggttg
     5401 ataccccgta cttcattgat gatgcgtgaa gacacgcgag ccagcaacgc atgcggcaac
     5461 ggcgcccaat ccgcagtcat gaaatcgaag gtctgcacgg cgcgcagggc caccacgtat
     5521 tcgtaggtgc ggccgtcgcc catcacgccc accgacttca ccggcaggaa gaccgcgaag
     5581 gcctgcgagg tgaggtcgta ccagctcttg cccgtttccg gatcgatgac ggcgcgcagt
     5641 tcttcgatga agatggcgtc ggcgcggcgc agcagttcgg cgaattcacg cttgacctcg
     5701 cccaggatgc gcacgcccag gccggggccg gggaacgggt ggcggtagac catctcgggc
     5761 ggcaggccga ggttgacgcc gagctcgcgc acttcgtcct tgaagagttc gcgcagcggt
     5821 tcgagcagct tgaaatcgag gtcgtcgggc aggccgccca cattgtggtg cgatttgatg
     5881 gcggcggcct tgccggtctt ggtgccggcc gattccacca cgtcggggta gatggtgccc
     5941 tgggccagcc acttggcgct cttcaacttg gccttctcgg cggagaagac ttcgatgaac
     6001 tcgcggccga tgatcttgcg cttggcttcg gggtcggcca cgccggccag cttgcccatg
     6061 aactgggcgg tggcatcgac gtggatgacc ttgacgccca tgttcttggc aaaggtgtcc
     6121 atgacctgct tgccttcgtc cagacgcagc aagccgtggt cgacgaacac gcaggtaagc
     6181 tggtcgccga tggccttatg gatgagggcc gcggccacgg acgagtcgac gccgcccgac
     6241 aggcccaaga taacctcgtc cttgccgacc tgttcgcgga tgcgggccac ggcctcggag
     6301 acgtagtcgg gcatgttcca gtcgcccgcc gcctcgcaga tgtcgcgcac gaagcgcgtg
     6361 agcagggcct tgccctgcac ggtgtgggtg acctcggggt ggaactgcac ggcgtagaag
     6421 ttgcgcgact cgtcggccat gccggcgatg gggcacgagg gcgtggaggc catgagcttg
     6481 aagcccgggg gcatttcgtc gacgctgtcg ccgtggctca tccacacctt gagcattccg
     6541 tggccctcgg gcgtggcgaa gtcctgcagg ccctcgagca gcttggtatg gccatgggcg
     6601 cggacttcgg catagccgaa ctcgcgatgg tcgtgatagg tgaccttgcc gcccagctgg
     6661 tgcgccatgg cctgcatgcc gtagcagatg cccagcaccg gcaggcccag ctcgaacacg
     6721 gcctggggca cgcgcatcga ggcctcgtcg taggccgagg catggctgcc ggagaggatc
     6781 acgcccttca agccgagctt ggcctggtcg cggatgaagt cgtccttgac gtcgccgggg
     6841 tggatctcgg aatagacgcc cgcctcgcgc acgcggcgtg cgatgagctg ggtgacttgc
     6901 gaaccgtaat cgagaatcag gatgcgctgg tgcatggagt tctgccaaca attgaaaaaa
     6961 gcagtgcaag aacgcaagcc catgcactgc caaggtctat ccagggcaca gcggatccgg
     7021 ctctgccggt ccgccagtgc cgccccctcg agggggcgcg cgaagcgcgt agggggtggg
     7081 ccctagtcgg cgcggtagtt gggggcttcc ttggtgatct gcacgtcgtg cacatgcgat
     7141 tcgcggaagc ccgccgaggt gatctggacg aactcggtct tggtgcgcat ctcgtcgatg
     7201 gtggcgcagc cgcagtagcc catcgaggaa cggatgccgc cgaccagttg gtagatgatg
     7261 gccagcacgc tgcccttgta gggcacgcgg ccttcgatgc cttcgggcac gaacttgtcg
     7321 gcgttgttgg ccgggtcctg gaagtagcgg tcggccgagc cgtcggtcat ggcgcctagg
     7381 ctgcccatgc cgcggtacga cttgtacgaa cggccctgga acagcacgat ctcgccgggg
     7441 gcttcgtcgg tgccggcgaa catgccgccc atcatgcagg cgtaggcgcc ggccgagagg
     7501 gccttggcga tgtcgcccga gaagcggatg ccgccgtcgg cgatgagcgg cacgccggtg
     7561 ccctcgagcg cgcgggccac atcggagatg gcggtgatct gcggcacgcc cacgccggcc
     7621 accacgcggg tggtgcagat ggagccggga ccgatgccga ccttgacgcc gtcggcgccg
     7681 tattcgacca gggcgcgggc cgccgccgcc gtggcgatgt tgccgccgat gacgtcgacc
     7741 ttggggaagt tctgcttgac ccagcgcacg cgctcgagca cgccgcggga gtggccatgg
     7801 gccgtgtcga ccaccagcac gtcgacgccg gcggcgacca gcttctcgat gcgttcttcg
     7861 gtgccgtcgc ccacgccgac cgcggcgccc acgcgcaatt ggccttgcgc gtccttgttg
     7921 gcgaccgggt gctcggtgtt cttgacgatg tccttgaccg tggccaggcc gcgcaactcg
     7981 aaggcgtcgt tgacgatgag gatgcgctcg agccggtgct tgtgcatcag cgcctgggct
     8041 tcgttgagcg tagtgccctc tttcatggtg atcaggcgtt cccgcggggt catgatgttg
     8101 cgcaggggct ggtcgaggcg gtcttcgaag cgcaggtcgc ggttggtgac gatgcccacg
     8161 agcttcctgc cctcgaccac cggcaggccg gagatgccgt tctggcgctg cagggcgatg
     8221 gcgtcgcgca ccttcatgtc gggcgtgacg gtgaccgggt cgatgacgat gccgaattcg
     8281 tggcgcttga cgcgggccac ttccttggct tgttcgtcgt ccgtgaggtt tttatggatg
     8341 atgccgatac cgccctcttg cgccatggcg atggccaggc gcgactccgt caccgtatcc
     8401 atggcggcgg acacgagagg aatgttcagg gtgatattgc gggtgagacg ggtggcgagc
     8461 gaggtatcgc gcggcaacac ctcggagtag gcaggcacca gcaacacatc gtcgaaggtg
     8521 agtgcttgtt ggacgagacg catggggaag ctccgggcgc aaagcaagat tatacgccta
     8581 ccttgcgatt tttcctaggg ttattaccta gggattacgt gacatatgcg ctcttgccgt
     8641 gcgtccctta ttgagttctt gagccgtccg ccgggagcgg gccaggggcg cggacacgtc
     8701 ggtgcggctc gggcgcgcca gcaggcgccc tcggccccta gcggggcttc ccttcctcct
     8761 actgtccgcg cccctggccc gcccggcgga ctctccatag gtcgaaaggc tacactttcg
     8821 ctatgtcgaa cacccatgaa atccgccccg ggcagtccat cgaactgctc aaggcgctgc
     8881 acatcctcac gcgcgacggc aagatgaacc aggacagccg ccgcaagctc aagcaggtct
     8941 accacctgtt ccagttcatc gagcctttgc tgcaagacgt gcaggacgaa cgcggcgcgg
     9001 tgacgctggt cgaccatggc gcgggcaaat cgtatctggg cttcatcctg tacgacctgt
     9061 tcttcaagga aaaggcccag gcgcggcaac agggcgtcga tgcgccccgc tcctcgcaca
     9121 tctacggcat cgaaacgcgc gaggagctgg tgcagcattc gcgcgagctg gcgcagcacc
     9181 tgggttttgc ccagggcatg tctttcctga acctgtcggt ggccgattcc atcgcgtcgc
     9241 aggccttgcc gcccacggtc gacgtggtga cggcgctgca tgcctgcgac acggcgaccg
     9301 acgatgccat ccgcttcgcg ttggagaaaa aggccaggca tgtggtggtg gtgccctgct
     9361 gccaggcgga agtggcgtcg gtgctgcgca agaacaaggc ccacgcgctg gcggacccgc
     9421 tggccgagat ctggcgccat ccgctgcaca cgcgcgagtt cggcagccag gtgaccaatg
     9481 tgctgcgctg cctgcagctg gaggcgcatg gctaccaggt gagcgtgacc gagctggtgg
     9541 gctgggagca ttcgatgaag aacgagctca tcatcgcgca gcgcaagcag gaccggccgc
     9601 ggcacaaggc cgccgagcgc ctgaaggaaa tgctggaacg catcggcctg cagcagctca
     9661 aggaccgttt cttcgcgccc gaaaccgcgt gacggctcac aggatctgct tgtagtagaa
     9721 cgtcgtcccg catggcgggc cgtccggcat cagtgcgtag ttcggcaccg tgccgacgcg
     9781 ctgccagcct gcccgggcat agagccgctc ggcgtcgccg ccggtgaccg tatcgagaac
     9841 cagcacggac ttgccttcct cccgcgcgac ttgctcgatc tcggtcatca gccgctgcgc
     9901 gatgccctgc cgccgggccc ggcgatggac cagcatcttg gcgacatcgg cccgatgcgg
     9961 ctggttctcc ggctgggcca tgacgagctg cacggtgccg acgatgcgcc ctgctccatc
    10021 ctcggcgacc agcaaggccc gctcgccacg ctcgacgccg tcggcgactc cgcgccagaa
    10081 ggccacggcc ttttcccggg agagcggctg catgaagctg accgaagcgc ccccttgcac
    10141 gcaatcgatc aggacatcgg ccagcatgtc cacgcaggcc cgggcctggc tggccgggac
    10201 gcggcgcacg cggagggtgt ctgtcatggc ttgctcctgg cttgattgaa atcgaggccc
    10261 gacagcgcca cgagatagcg cgccggcttg cgggcagggt tgtgataggc gatgggacgg
    10321 tcgagccgca tggccaggca gtcgccggtt tcgagccgcc agcactgttc gccgacggtc
    10381 acttccatgg tcccttcgat catccatacc tgctggtgga tcccggtgtc gcgcacgtcc
    10441 gtgtcaaagg cgacatgctg tccaggcggg aagcgaacct cgaccaattg gaggggcgac
    10501 ggccaggcgg gagacaggtt gcggcggaca tagccggagg ccggatcgga ccatgccggc
    10561 tgctccgccg cgcgggagac gggcaaaggc gcggcagcgg gctgcgcgta aggctcgaac
    10621 agcgacacga ccgccacgcc cagcgccgaa gccagcttgt cgagcaccgt cgcggtaggg
    10681 ctgctttgcc cgcgttcgat cagcgagatg gccgaccggc ttaccttgct gcgctcggcc
    10741 agcacatcga gcgaccagcc acgcgcgtcc cggcgctcgc gcacacgctg tgcgatcagg
    10801 ctgttgatgt ccatgatatc tagttttatg tacaaaaaat ctagtaaact ggatattcat
    10861 gccaagcccg gaccctgtca agcaaaacgc ggggaatctt ccggctcttc ccggccaggc
    10921 ctgcaagcct gcacacgcgc gaaacccgtg tgcagcgccc tagtccatca atttgcgcca
    10981 accgtcgagc cccagcttct ccatggtctc gatgttcttc tcgtagatgg catcggcctc
    11041 gggaaaggca tcgaccgcgc gatcgatgct ggcttcgcgc aggatgtgca ggatgggata
    11101 cggcgaacgg ttggtgtagt tgccggcgtc gccgggatcg gtatcggcaa actggaaggc
    11161 cggatggaaa gtggccacct gcaggtcgcc caccaggcgc aggcgcttga gcaggcggtc
    11221 ggacagctcg ccgaaatcgt tgaactcgta gaaatcgtgc aaggcctcgg gcaggatgag
    11281 cagcgtggtg tcgacctttt ccggatcggc ctcggcgatg agcaggagct cgtcctccag
    11341 gtcggccagc acgccctcct cgtccgtcgc gtcgctcacg gcatagcgca cctggctctt
    11401 gatctgcacg gccttggcga agggacagag gttcagcccg atgacggcct tttccaccca
    11461 gcgcagcgtg tcggccgcca cctggcgggc agcgtcgtcg tcgacgatca tgccggcatg
    11521 tcggccatgg tctgcgccac ggccgcggtg attttcttgc cgtaggggat atgcaggaac
    11581 tcgttggggc cgtgcgcatt ggacctgggg cccaacacgc cgcacaccat gaactgcgcc
    11641 ttgggaaagc cctgctgcag aatgctcatg agcgggatgg tgccgccctg gccgatatag
    11701 ccgcaaggag cgccgtagta ctgccgcgaa gcgcggtcga gcgcggccga cagccagggc
    11761 gccgtggccg gcgcattcca gcccgtggcg gcgccctggt tggcatggaa gatgaccttg
    11821 gcgttatagg gcgcgtcgtc ttcgagcagg cccttgattt cctgcgcggc ggccaccgcg
    11881 tcgatcaggg gcggcaggcg cagcgacagc ttgaaggcgg tgcgcgggcg cagcacgttg
    11941 ccggccgagg acaggggcgg caggccttcg gcgccggtca ccgacagcgt aggccgccag
    12001 gtgcggttga gcagcgcctc ttcgggctcg gcggtcatgg gcagcacgaa gctgccgtcg
    12061 gcgccgcagc tccaggggaa acggcgccag acctcctcgc ccaggatctt ggccgtggcc
    12121 ttgacctgct cgacgcgttc ggcggggatt tcgcaatgca ggctctgtgg caacaggcgg
    12181 ccggtgccgc tgtcttccaa gcggtcgagc agatggcgca ggatgcggaa ggtcgacggc
    12241 accacgccgc tggaatcgcc cgagtgcacg ccctcgtcga gcacctgcac ttccagcgtg
    12301 cccgagacca tgccgcgcag cgaggtggtc atccacagct ggtcgtagct gcccgcgccc
    12361 gagtccaggc acaccaccag ggccacgttg cccagccggc cgcgcagcgc gtccacatag
    12421 ggcagcaggt cgtagctgcc ggattcctcg caggtttcga tgatgcccac gcagcgcggg
    12481 cgcggcacgc cctgcttgtc gagcgccatg atggcggtga tcgacgcata gacggcatag
    12541 ccgtcgtcgg cgccgccgcg gccgtagagc ttgtcgttct cgagcttggg cgtccagggg
    12601 ccgaggtcgt tgcgccagcc ggagaactcg ggctgcttgt cgaggtggcc gtacagcatg
    12661 atggtgtcgc cgttgtcggc gcgggtggcc ggcgcgtcga aaaagatcac cggcgtgcgg
    12721 cccggcaggc gcacgatctc gagcacgagg ccgtggatct tctgcgcttc gacccactgc
    12781 gccgcgtcgc ggatgacgcg ctcgatgtag gcgttctttt cccagtcggc gtcgaaggac
    12841 gggctcttgg ccgggatctc gatataggtg gtcagcgcgg ggatgatctc gccatcccat
    12901 ttttcgttga cgaaggcctg cagggcgtcg gggtcgaggg tgggcggcag ggcgtcgtcg
    12961 ggaatgcggg cgttcatggg aggatcctca aaagcagtcg ctgtgccgac attttatgtc
    13021 cagccgcaga cgtctcgcca acgggcctaa cccctaggcc ttctctttgg cgtggccccg
    13081 tgacaagttt gtccaactcg ggatccacga atggcgccac gaagcctcca aattcaaaat
    13141 gctgtcccag gccgcatgcg gaaccactcc cggttttgac cactgcgtgc taacttataa
    13201 attcctgaac gggatacgga tgatgagcga tatggcaata cgctttgata ccctcaagat
    13261 tgttgaacgc ctcgagcgag gcgggttcac gcgtgagcag gccaaaactc aggctgaggt
    13321 gctggccgac gtcatcaacc ccgatgcgct gggctttgct acacggcagg atctgcaaga
    13381 cgtgcgtggc gaactgcgca atgaactgca cgaagtcgaa taccgcctcg tcgcccggat
    13441 caccaccgtg gaacacaaac tcgagaattt ccagaaagcc acggaaggca agttcaacct
    13501 gctttattgg atgatgggct tcatgctcac catgatgagc ggcctgttct acaaactggt
    13561 catcctgtag aagtccatcg tcccactatc ctggcaatga aaatggccgc ccaatagcgg
    13621 ccatttgcac gccagctcgc acagccttac ttcagccaca cccgcgcatt gcggaaaatg
    13681 cgcatccacg gcgagaactg gccgccggtg tcggcggtgc cccacttctc gggcgcccag
    13741 gacatcatca cattgcgggt gacgcgttcg gggtgcggca tgatgaccgt gaagcggccg
    13801 tcggccgtgg tgacggaagt caggccgcca ggactgccgt tggggttgta ggggtagtct
    13861 tcggtgcggt tgccgtagtg gttgatgaaa tgcgccgcgc cgatgacctt gccggcattg
    13921 ccctgctgcg agaaatcggc atagccctcg ccgtgcgcca cggccaccgg gacgcgggtg
    13981 cccgccatgc cggcaaagaa gagcgagggc gactcggcga tttccaccag cgacagacgg
    14041 gcttcgtact tttcggactg gttgcgcgtg aagcgcggcc aatgcccggc gcccgggatc
    14101 atgggcgcga gcgccgccat catctggcaa ccgttgcaca cgcccaggcc gaaggtgtcg
    14161 gggcgcgcga agtactcggc gaactggtcc gacagcttgc tgttgaagcg gatggtgcgc
    14221 gcccagcctt cgccggcgcc cagcacgtcg ccgtagctga agccgcccac ggccaccagg
    14281 ccctgcatgg tggagaggtc gatgcggccg gccagcaggt cggtcatgtg cacgtcgacc
    14341 gcctcgaagc ccgcggtgtc gaaggcccag gccatttcga cctggctgtt gcagccctgc
    14401 tcgcgcagga tggccacgcg cgggcggcgg ccggtggcga tgaagggcgc ggcgatgtcg
    14461 tcctgcggat tgaaagccac gcgcggcgac aggccggggt tggcgacgtc cttccaggtg
    14521 tcgaactcgg cctgggcgca ggccggattg tcgcgcagca tcgcgatgcc gtggctgact
    14581 tcgctccagg cgctgcccag ctcgggacgc ggctggctcc agaccttctt gccgtcgcgg
    14641 tagaactcga tctcgtcgtg gccgttgagc ccgccgatga catgcgagta agccgacagg
    14701 cccgcgccgc gcagcacctg catgacggcg tcgcgctgcg acagcggcac ctggatcacg
    14761 gcgccggcct cttccgagaa cagggccttg agcgtgagct cctcgcgctg cacggccacc
    14821 tggtcggcgc ggatcttgta gtcgccccag tcggccgaat tggggtcgaa ggtgagcatg
    14881 tcgaggttga ccgagatgcc ggtgcggccg gccagggcca tctcgaccag ggtggcgaac
    14941 aggccgccgt cagagcggtc gtggtaggcc aggatgatgc cggcctcggc cagcgtgcgg
    15001 atggtgatga agaaggcgcg caggtcctgc gggcggtcga tgtcgggcac cccgtcgttg
    15061 tcacccccgc cgacctgccc ataggcctgg gccaggatgg agccgcccat gcgatggcgg
    15121 ccctgcccca ggtcgatgag gatgagcgcg ctgtcgccga tatcggtgcg cagctgcggc
    15181 gtgaggctgg cgcgcgcgtc cttcacgggc gcgaaggcgg taacgatgag cgacaccggc
    15241 gacaccacct ggcggacctc ggcgcctgcc gtgccgtcga ccgtccacga ggtcttcatg
    15301 gacagcgaat ccttgcccac ggggatcgac aggcccacgg cctggcatag ttcgctgacg
    15361 gccgtgacgg tgtcgtacag cgcggcatcc tggcccggcg cgccgcaggc ggccatccag
    15421 ttggccgaga gcttgatatc ctcgacctgg gccacgtcgg cggcggccag gttggtgagc
    15481 gcctcggcca cggccatgcg gcccgaggcc ggcgcgtcga tcacggcgat gggcgtgcgc
    15541 tcgcccatgg acatggcctc gccgcggaac ccttcgtagt cggccagcgt gacggcgcag
    15601 tcggccaccg gcacctgcca ggggccgacc atctggtcgc gcgccgacag gccgcccacg
    15661 gtgcggtcgc cgatggtgat gaggaaggtc ttgttggcca ccgtgggatg gcgcagcacg
    15721 cggtaggcgg cctcggtgag gtcgatgccg gccaggtcga gcttgccggc cgcgccgggc
    15781 aggcgccgca cgtcgcgcgt catgcgcggc ggcttgccga ggatgacgtc gatgggcacg
    15841 tccaccgggc gcacctcgcc gtcgggcttg gcccgcggcg cagttccgtc accctccagg
    15901 cccggcaggc cttcgccgtc gaccacgcgc agcgcgcgct cttcggtggc cacgccgacc
    15961 acggcgtagg ggcagcgctc gcggcgcgca atggcctcga agcgttccag gtcccgcggc
    16021 agcaccgaga gcacatagcg ttcctgcgac tcgttgctcc agatctcggc gggcgacatg
    16081 ccggattctt cgaccggcac gcgcttcagg tcgaacaccg cgccgcgcgc cgcatcgttg
    16141 accagttcgg gaaaggcatt ggacaggccg ccggcgccca cgtcgtggat ggcgatgatg
    16201 gggttgcccc ggcccagctg ccagcagcgg tcgatgacct cttgcgcgcg gcgctcgatc
    16261 tcggggttgc cgcgctgcac ggagttgaag tccaggtcgg ccgaattgct gcccacgccc
    16321 atgctggacg cggcgccgcc gcccatgccg atgcgcaggc ccgggccgcc cagctggatg
    16381 agcagcgcgc ccggcgggat ggcgtccttg tgcgtgaggc tggcgtcgat gctgcccagg
    16441 cccccggcga tcatgatggg tttgtgatag ccccagcggg tttgctgcgc cccttcgccc
    16501 gcggtctgct ccgtcgtttg ctcgtacgtg cggaagtagc ccagcaggtt gggccggccg
    16561 aattcgttgt tgaaggccgc gccgcccagc gggccgtcga tcatgatgga cagcggcgtg
    16621 gcgatgcgct cgggcaggcc gtgatggtcg gcctcccagg gttgctcggc gccgggcagg
    16681 cgcaggtgcg acacggtgaa gccggtcagg cccgccttgg gcttggagcc gcggcccgtg
    16741 gcgccttcgt cgcggatctc gccgccggcg ccggtggagg cgccggggaa cggcgcgatg
    16801 gcggtcgggt ggttgtgcgt ctcgaccttc atcagggtat gcacggtggt ctcgcggcgg
    16861 cgatacaccg ggctcgcccc ggccaggccg ggaacgccgg cctcgaagcg ctgcgccgcg
    16921 ccgccttcca tgacggcggc gttgtcggaa taggccacca ccgtgccgtt gggctgcgcc
    16981 ttgtgcgtgg cgcggatcat gccgaagagc gtgtggggct gatcggcacc gtcgatcttc
    17041 cattgcgcgt tgaagatctt gtggcggcag tgttcgctat tggcctgcgc gaacatcatc
    17101 agttcgacgt cggtcgggtc gcgcctgagc gcggcaaacg catcggccag gtaatcgatt
    17161 tcgtcttcgg acagggccag gcccagcgcg gcgttggcct cgaccagcgc ggcgcggccc
    17221 tgggcctgca gcggtatcgt gcgcaccggc ttgcccgcca gcggcgcgaa cagcgcctgg
    17281 ccgtcgaagc cggcgtcgac cacggtttcg gtcatgcggt cgtgcaggca ggcggccgcg
    17341 cgggccaggg tgtcggcctc gaaggacttg ctgcccagca agccacgctc aggcgtcagc
    17401 gtgtagcgga tgccgcgttc gatgcggcgc accgccgaca ggccgcagtt gcgggcgatg
    17461 tcggtggcct tgctggccca gggcgacacg gtgcccaaac gcgggatgac cagcagttcg
    17521 agcgccttgg ccggcgcgtc ggaggccgcg gccggcgtgc cgtagtccag caggtcggcc
    17581 aggcgctggg cgtcttgcgc gccgagctcg gattcggtga ggacgaaatg ctcgtagcgg
    17641 gccgagacgt cggccaccgg caggccggcc tgcttcaact gggcaagcag gcgttcgcgg
    17701 cggaaagcgg aaaggacgga ggaaccgggc agatgctgga cgagggacac gatgcggccg
    17761 aaggggtggg atcagaaagc ggtggatcaa aagacgattt tacctttttg ccccagcccc
    17821 tggcgccgcg cctgcccggg catcagaccg gatcagccgg cctgcacctc gtagccggcc
    17881 tcttcgatgg cggccctggc ctgggcggcg tcgacgccgt cgatgacgac ccggtggctg
    17941 gccaggtcgg tcttgacctg cgccgcgggc gccacggcct ggacggcggc cgtgatggtc
    18001 ttgacgcagt ggccgcagct catgtcggga acaaggaatt cggtggtcat gcggggactc
    18061 ctgtagaaga ttcggagcca gcttaaacct tcccatcatg gcaatgtcaa gcctacaatg
    18121 aaccttgacc ttcccataat gggaaggtgg acactggctt cctgcttccc cacaggcgcg
    18181 acgcccgaga agaacatgaa caccctcgcc accacagaac tcgaactcgc tgtttccggc
    18241 atgacctgcg cctcgtgcgt gcgccgcgtc gaaaaggcgc tggccaaagt gcccggcgcc
    18301 cgggaagcgg ccgtcaacct ggccaccgag cgcgcccagg tacgctacga cccggccgaa
    18361 ggcagccccg acgccttcgt cgccgccgtc gccaaggcgg gctaccaggc ccggcccatc
    18421 gtcgcgcagg acaaccaggc ccgcgaactg gccgagtcgc gcgcagccga ggcgcggcat
    18481 ctgcgccgcg atttcctcat cgcgctggcc ttgtccctgc ccgtgttcat cctggagatg
    18541 ggcggccacc tgctgcccgc ctggcaccac tggatcgccg ccacgctggg cgaacgcaac
    18601 ggcttctacc tgcaattcgt gctgaccagc gcggtgctgc tgggtccggg ccggcgcttc
    18661 ttcagcagcg gcctggcggc cctgtggcgc ctggcgcccg agatgaacac gctggtggcg
    18721 ctgggcgccg gggcggcctg gctctattcc agcgtggcca ccttcgcccc gcatgcgttg
    18781 cccgacagcg cgcggcaggt gtatttcgaa tcggccgccg tcatcgtgac gctgatcctg
    18841 ctgggccgca tgctcgaagc cgctgcccgc ggacgcacgg gcgcggccat ccagcggctg
    18901 gtgagcctgc agccgcagac cgcccgcgtg ctgcgcgacg gccagccgca ggacctgccc
    18961 atcgaacaac tgattccgga cgatgccgtg ctggtgcggc ccggcgaacg catccccgcc
    19021 gacggcacgg tgaccgaagg cgcttcgtac gtggacgaat ccatgctcac cggcgaaccc
    19081 ctgcccgtgg aaaaaacggc gggggctccg gtcaccggcg gcacgctcaa caccaccggc
    19141 agcttcaccc tgcgcgtctc gcacctgggc gccgacaccg tgctggcgcg catcatccgc
    19201 atggtcgaga ccgcccaggg cgcgcgcctg cccatccagg ccctggtcga ccgcgtcacc
    19261 ggctggttcg tgccggccgt gctggccgcc gccctcctca cctttgccgc ctggatgatc
    19321 tggggacccg accccgccct gccccatgcc ctggtcaatg ccgtggccgt gctcatcatc
    19381 gcctgccctt gcgccatggg cctggccacg ccgacctcca tcatggtggg caccggccgc
    19441 gccgccgagc tgggcgtgct gttccgccag ggcgatgcgc tgcaggccct gcgcgacgtg
    19501 gacgtcgtgg ccttcgacaa gaccggcacg ctgaccctgg gcaaaccgac catgaccgac
    19561 tggacggccc ccggctgcga cgagtcgctg ctcctgcagc ggctggcctc ggtgcaggcg
    19621 cgctcggaac atcccatcgc gcgcgccatc acggccgcgg cgcaggcccg cggcctggcc
    19681 ccgctgccgg ttaccggctt ccaggccgtc accggcgccg gcgtaacggc gcaggtggac
    19741 ggccggctgc tcatcgccgg ttcggcgcgc ctgatggcgg aactcggcat cgacctctcg
    19801 gccttcggca accaggcggc cgactggggc cgcgacggca agacgccgat ctatgtggcc
    19861 gtcgacgggc aggcgcaggc catggccgcg gtggccgacc ccttgcagcc ctcggctgtc
    19921 caggccatcg cggccttgca cgcgcaaggg ctgtccaccg cgatgatcac cggcgaccat
    19981 cgcgacaccg cgcaagccgt ggcgcgccag ctgggcgtgg acgatgtgcg cgcggaagtc
    20041 ctgcccgaag gcaaggtcca ggccatcgaa gccctgcgcc agggcgggcg caaggtgctc
    20101 ttcgtcggcg acggcatcaa cgacgcgccg gcgctggcgg ccgccgacat cggcatggcc
    20161 atcggcagcg gcaccgacat tgccatcgag gcggcgcacg tggtgctgct gggcaaggac
    20221 ctgcgcggcg tggccgacgc catcgcgctc agccgcgcca cgctgcgcaa tatccgccag
    20281 aacctgttct gggcctttgc ctataacgtc gcgctcattc cgctggcggc cggcgccctg
    20341 tatccggccg ccggctggca gctctcgccc atgctggcgg ccggcgccat ggccctgtcc
    20401 agcgtgttcg tcgtcagcaa cgccctgcgc ctgaaaacct tccagcccgc gagggtccgc
    20461 cctgcccggg caggcgcctg aacccaggga aacgcgccat gaacatcgga caagccgccc
    20521 aggccaccgg catctcggcc aagatgatcc gctactacga gaccatcggc ctcatcggcc
    20581 cggccggccg cacggccgcg ggctatcgca cttataccga gcaggacctg cacaccctgc
    20641 gcttcgtgcg ccgcgcgcgc gggcttgggt tttcggtgga gcagatgcac gagctgctgg
    20701 ccctgtggcg cgaccgcaag cgcgccagcg ccgacgtcaa gcgcgtggcc ctgcagcatg
    20761 tggaagaact ggaacgcaag gcggcggaac tgcagcagat ggccgacacg ctcaagcacc
    20821 tggcccggca ttgccacggg gatgagcggc cgg
//
LOCUS       DASWPO010000155         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_658826, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000155 DASWPO010000000
VERSION     DASWPO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658826"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1204)
                     /locus_tag="VGC69_08625"
     CDS             complement(41..1204)
                     /locus_tag="VGC69_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0295377.1"
                     /translation="MRSLQIKTWYAVHKWTSLVCTLFLLVICLTGLPLVFQHEIEAGF
                     RTGKAPAALPAGTPRASLDRVAGQVLQRHPGERIDYLYLDPDEPTVLVGLVPPSASGG
                     GHAVHVDARTAEVLLDGPSYEAARFSFMGIMLALHTDLYAGLAGELFLGSMGLLFVVA
                     IVSGVVLYGPFMKKLEFGTVRAARSRRIKWLDLHNLLGIVTLAWGGVVGVTGVINELS
                     TPLFSLWQRNTLPALLAAAGAPAPAGAGPASPQRVAEVALEAVPGMSLTSMNYPGSPY
                     GSPRHFIVWLQGNTPLTSRTHTAVLVDGASGSLVKVAALPWYLTALEVSRPLHFGDYG
                     GLPLKVLWALLDLATIVVLASGLYLWIARRKAGDARLAELARRRQAEVRENAA"
     gene            1294..>1516
                     /locus_tag="VGC69_08630"
     CDS             1294..>1516
                     /locus_tag="VGC69_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0295378.1"
                     /translation="MRFRLRPLALFIAALPCQAALAQAQTTPAPANGDQPAVMETITV
                     QASADASGVGLPPAYAGGQVAQGGRVGVLG"
BASE COUNT          275 a          532 c          504 g          205 t
ORIGIN      
        1 aggatcggca tgccccagac ggccccgaag cgccgcgcgc tcacgccgcg ttctcccgga
       61 cctcggcttg acggcgccgc gccagctccg cgagccgcgc atcgccggcc ttgcgccggg
      121 cgatccacaa atacaggccg ctggccagca ccacgatggt ggccagatcg agcaaggccc
      181 acagcacctt cagcggcagg ccgccgtaat cgccgaaatg caagggccgg gagacttcca
      241 gcgcggtcag gtaccagggc agggcggcca ccttgaccaa agagcccgaa gcaccgtcga
      301 cgagcaccgc cgtgtgcgtg cgcgacgtga gcggcgtgtt gccctgcagc cacacgatga
      361 aatgccgcgg actgccgtag gggctgccgg gatagttcat gctggtcagc gacatgccgg
      421 gcacggcctc gagcgccacc tcggccacgc gctggggtga agccgggccg gcgcctgccg
      481 gcgcaggcgc gccggcggcc gccagcaggg cgggcagggt attgcgctgc cagaggctga
      541 aaagcggcgt cgacagttcg ttgatcacgc cggtcacgcc gaccacgccg ccccaggcca
      601 gggtcacgat gcccagcagg ttgtgcaagt ccagccactt gatgcgccgg gaccgcgccg
      661 cgcgcacggt gccgaactcg agcttcttca tgaaaggccc gtagagcacc acgcccgaga
      721 caatggcgac gacgaagagc aggcccatcg agcccaggaa caactcgccc gccaggccgg
      781 catagagatc agtgtgcagg gccagcatga tgcccatgaa actgaagcgc gccgcctcgt
      841 aggacgggcc gtcgagcagg acctcggccg tgcgcgcatc cacgtgcacg gcatggccgc
      901 cgcccgaggc ggagggcggc accagcccca cgagcaccgt cggctcgtcc ggatccaggt
      961 acaggtagtc gatgcgctcg cccgggtgcc gctgcaagac ctggccggcc acgcgatcga
     1021 ggctggcgcg cggcgtgccc gcgggcaggg cagcgggcgc cttgcccgtc ctgaacccgg
     1081 cttcgatctc gtgctggaac accaggggca ggccggtcag gcaaatcacc agcaggaaaa
     1141 gcgtgcagac caggctcgtc catttgtgaa cggcatacca agtcttgatt tggagggagc
     1201 gcatcgccga ataatattcg aaaagataat gataatgatt cttttttgat attatctctc
     1261 ccatcgattc acctcctttt cagcagaccg cgcatgagat ttcgccttcg ccccctagcc
     1321 ctgttcatcg ccgcattgcc ttgccaggcg gccctcgcgc aggcgcaaac cacccccgcg
     1381 cccgccaacg gcgaccagcc ggccgtgatg gaaaccatca ccgtccaggc cagcgccgac
     1441 gcctcgggcg tcggcctgcc gcccgcctat gccggcggcc aggtggcgca gggcggccgg
     1501 gtgggggtgc tgggca
//
LOCUS       DASWPO010000156         9754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_213894, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000156 DASWPO010000000
VERSION     DASWPO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213894"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VGC69_08635"
     CDS             <1..944
                     /locus_tag="VGC69_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295379.1"
                     /translation="LAEGEQVSLFFGGLKSDAGLALASYAGQQQAFYLATLPMSSRLT
                     WEQGNAYTYRLAPSGWMQVAALVPRTFGLRKRRWALVYPDDAYGQPTAAAFRSMITSF
                     QSRTTIVSDQAVAPGKFDARELVRRLREDKPDAILNLLTGPQLARFVHEGKAQGLLQD
                     MAMVAPLAGEPEALQAMGADASAGWLVSGYPCEPADAATLPRFDRDYQARYGSAPGAA
                     SALGYIALRALAEGLRKAGEAGPQAVAAAFAGLNLDTPYGPVQFRAIDHQATLGVCLA
                     TTAEQDGRIVAQPAGYLDGSRLQPPDKTVHRLRGKQQ"
     gene            1095..1514
                     /locus_tag="VGC69_08640"
     CDS             1095..1514
                     /locus_tag="VGC69_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0295380.1"
                     /translation="MATKKAPAKKVVTKTVKKAAVKPAAKKAAAPAKKAGPIKAALNK
                     SQLVAYLVESTGVEAKSVKAVLAGLETATLSSVDKKGAGSFTLPGLFKIAVQKVPAKP
                     KRFGKDPFTGEERWFPAKPASVKVKVRPLKKLKDAAQ"
     gene            1785..4454
                     /gene="leuS"
                     /locus_tag="VGC69_08645"
     CDS             1785..4454
                     /gene="leuS"
                     /locus_tag="VGC69_08645"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY0295381.1"
                     /translation="MQERYQPNSVEAAAQQDWQARNAYLVSEHVKNADGSEKPKFYAC
                     SMLPYPSGKLHMGHVRNYTINDMMARQLRMRGYNVLMPMGWDAFGMPAENAAIKSKVP
                     PAKWTYDNIAYMKKQMLAMGLAIDWSREMCACDPKYYKWNQWLFLKMLEKGIAYRKTQ
                     VVNWDPVDQTVLANEQVIDGKGWRSGAPVEKREIPGYYLRITDYADELLREVQTGLPG
                     WPERVRVMQENWIGKSEGLRFAFPHRIQGADGRLIQDGRLYVFTTRADTVMGVTFCAV
                     APEHPLATHAAQSDAKLAAFIEECKLGGTTEAELATREKSGLPTGLSVTHPITGEQIP
                     VWVGNYVLISYGDGAVMGVPAHDERDFAFARKYGLAIKQVVALEGKTYSTDAWQEWYG
                     DKQAGKTINSGKYDGLTHKEAVDAVAADLAPQGLGEKQTTWRLRDWGISRQRYWGTPI
                     PIIHCADCGPVPVPEKDLPVVLPEDLIPDGSGNPLAKHEAFLACACPKCGKPARRETD
                     TMDTFVDSSWYFMRYTSPGNDGAMVDARNDYWMPMDQYIGGIEHAVLHLLYARFWTKV
                     MRDMGMLKFDEPFTKLLCQGMVLNHIYSRRNEKGGIEYFWPEEVENLYDAKGVITGAQ
                     LKSDGSPVDYGGVGTMSKSKNNGVDPQALIDHQGADTARLFVMFASPPEQTLEWSDSG
                     VDGANRFLRRLWSLAYAQREAVARGLAAGLDWQQAPAPVKDLRREVYGLLKQADYDYQ
                     RIQYNTVVSAGMKMLNAIESARLPADGTAGAATDAALAETLGILLRVLYPVVPHVTWQ
                     LWRDLGYAHELGDLLDAPWPHVDEAALVADEIELMLQVNGKLRGSLRMPAQAAKEDIE
                     QQAAAHPETARFLEGRPAKRVIVVPGKLVNVVG"
     gene            4507..5127
                     /gene="lptE"
                     /locus_tag="VGC69_08650"
     CDS             4507..5127
                     /gene="lptE"
                     /locus_tag="VGC69_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY0295382.1"
                     /translation="MVSAAGLALLTLLAACGFQMRGVTPLPFDTLYVGVSDTTKFGAD
                     LRRALRAASPGTRLVDSPKDAQVLLQQVSSQRLIDDTALNAQGRVEEYELSVYFTFRV
                     IDAKGNAILGDTAFSAFRELPYNDQAAQALSSQIDSLYASMEQSLINRVLRRLNAIDV
                     RENYARLQRGEVNPDRPVFDPTKVAPVPAMPPGWNSPTNNLGGPPR"
     gene            5178..6242
                     /gene="holA"
                     /locus_tag="VGC69_08655"
     CDS             5178..6242
                     /gene="holA"
                     /locus_tag="VGC69_08655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY0295383.1"
                     /translation="MATALDASSLASHLARNGQSLAPLYTVSGDEPLLVIEAIDALRA
                     AARQAGYADRTSLVMDARSDWSAALAATQTVSLFGDRSLLEIKLDSGKPGKTGGETLV
                     RLAEQARVQAQTQANPDVLVIIALPRLDRDTRNSKWAQALAAGGVMVDIATVDRPQLP
                     AWVGARLARQNQRADNATLQWMADKIEGNLLAAHQEIQKLGLLYPEGELSAEDVERAV
                     LNVARYDVFGLRDAMLAGDSARTLRMLEGLRAEGEAEPLVLWAVGEEIRLLSRLADAR
                     QQGQDVGGLMRRLRIFGNHERLALQAISRVSPKSWPAAVQHAHDVDRLIKGLPTSGRM
                     SDAWEEMARLALRVAAAGTR"
     gene            6405..7661
                     /locus_tag="VGC69_08660"
     CDS             6405..7661
                     /locus_tag="VGC69_08660"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY0295384.1"
                     /translation="MSIEQTMLALGQRARTASRHTMLADTGQKNQALLAMADAIAEHR
                     GELQAANAADVADARRDGLDAALLDRLTLSDRHIGQMQEGLRQVAALPDPVGGITAST
                     LRPNGMRVAQMRVPLGVIGIIYESRPNVTIDAAALCLKSGNAAILRGGREALRSNLAL
                     AKVVRQGLAAAGLPPEAIQVVETSDRAAVGALITMTEYIDVIVPRGGKGLIARLAQDA
                     RVPLIKHLDGNCHVYLDSAADPARAHEIAYNAKTYRYGICGAMETLLVHAAIAPAVLP
                     GLGRALHDHGVELRGCARTRALLPYAVAATEEDWGTEYLGPILAIKIVDNVDEAMAHI
                     ARWGSGHTDAIVTENLATARQFQREVDSSSVYVNLPTCFADGFEYGLGAEIGISTNRL
                     HARGPVGLEGLTTLKWVLEGNGQIRG"
     gene            7668..8072
                     /locus_tag="VGC69_08665"
     CDS             7668..8072
                     /locus_tag="VGC69_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295385.1"
                     /translation="MQNLPLVPVFQLTFSLFVLCFAACTVCGLLLFYFKSDQVNNVMQ
                     HPYLQHKPFKQLPFTIRATILLDYYLHLVFPKRRFWVIGQANYLLQHVDPAQVPKGVK
                     WLICSFWGSCWLGLIDMAVLWGVLSLGAGLGL"
     gene            complement(8069..8935)
                     /locus_tag="VGC69_08670"
     CDS             complement(8069..8935)
                     /locus_tag="VGC69_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295386.1"
                     /translation="MDWDNARTFLALYRSGTLRAAAVALQVDQATVGRRLAALEASLE
                     ARLFLRTPRGYVPTTAGEMAYAAAERMEQAADQLARQMQGLDRRLSGVVRVATSDTIG
                     RHFLMQAIAQVQAQHPDIQVVLATSPHVTNLTRREADLAVRVVRPDNPELIVRHLSRR
                     TVGLYATQDYLQRYGEPRPGEAFAGHRLVAYERGLLPQRPDTFCGEPTHNAVIAVEVN
                     SGLLMMEAVASGLGIGELPNHLTQEFAQLVRLWPRREEGYDIWLVMHSDLHRTARVRA
                     VADAIVESFAQA"
     gene            9075..>9754
                     /locus_tag="VGC69_08675"
     CDS             9075..>9754
                     /locus_tag="VGC69_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295387.1"
                     /translation="MSSSCPAIPASPALPAHDPSLLPLATLAIGFVMAMLDVTVVNVA
                     LPSIASQFNTPLTNLVWIVDGYTLTFAALLLVAGALANRFGAKRIYLTGLAVFTLASL
                     LCGLSLNAGMLIGARLVQGFGAALFMPSSLSLLAHAYDNDTVRTRMLSLWSAIVSIAA
                     AAGPLVGGLLIDTFGWRSVFLVNIPVGLIGLWLASSRIAAAPRHPHPLSITSHALGVL
                     FLGSICYAL"
BASE COUNT         1678 a         3612 c         2981 g         1483 t
ORIGIN      
        1 aactggccga gggcgagcag gtctccctgt ttttcggcgg cctgaagtcg gacgccggcc
       61 tggccctggc cagctatgcc ggccagcagc aggccttcta cctggccacc ctgcccatgt
      121 ccagccggct cacctgggaa caaggcaatg cctacaccta ccgcctggcg ccatcgggct
      181 ggatgcaggt ggccgcgctg gtgccccgga cattcgggct gcgcaagcgg cgctgggcgc
      241 tggtctatcc cgacgacgcc tacggacagc cgaccgccgc ggcctttcgc agcatgatca
      301 ccagcttcca gtcccggacg accatcgtgt cggaccaggc cgtggcgccg ggcaagttcg
      361 atgcgcgcga gctggtgcgc cgcctgcggg aggacaagcc cgacgccatc ctcaacctgc
      421 tcaccggccc gcaactcgcc cgcttcgtgc atgaaggcaa ggcgcagggc ttgttgcagg
      481 acatggccat ggtcgcgccg ctcgccggcg agcccgaggc actgcaggcc atgggcgccg
      541 atgcttcggc gggctggctg gtcagcggct atccctgcga gccggccgac gccgcgaccc
      601 tgccgcgctt cgaccgggac taccaggcgc gctacggcag cgcgcctggc gcggcatcgg
      661 cgctgggcta catcgccctg cgcgccttgg ccgaaggcct gcgcaaggcc ggcgaggccg
      721 gcccgcaagc cgtggccgcc gcctttgccg gcctcaacct cgacacgccc tacggccccg
      781 tgcaattccg cgccatcgat catcaggcca cgctgggcgt ctgcctggcc accacggccg
      841 agcaggacgg ccgcatcgtc gcgcagccgg ccggctacct ggatggctcg cggctgcagc
      901 cgcccgacaa gaccgtgcac cgactgcgcg gaaaacaaca ataaatacgg gctcgtagcg
      961 cgcaaatgca cgaattcccc gcgagttccg ctattatcgg gcgctaggca gcctgtgctg
     1021 gcaaaggccc attaacaagt ggtccgttgc aagcgccgtg cagtctcatc ctcacccttt
     1081 tcatggattg gatcatggca accaaaaaag cccccgccaa aaaagtcgtc accaagactg
     1141 tcaaaaaagc tgctgtcaaa cccgcagcca agaaagccgc cgcccccgcc aagaaagccg
     1201 gccccatcaa agcagccctg aacaagtcgc aactggttgc gtacctggtc gagagcaccg
     1261 gcgtcgaagc caagtcggtc aaggccgtgc tggccggcct ggaaaccgca accctgagtt
     1321 cggtcgacaa gaagggcgct ggctcgttca cgctgcccgg cctgttcaag atcgccgtgc
     1381 agaaggttcc cgccaagccc aagcgcttcg gcaaggaccc cttcaccggc gaagagcgct
     1441 ggttcccggc caagccggct tcggtcaagg tcaaggtgcg cccgctcaag aagctgaagg
     1501 acgccgcgca gtaattcctg cgacgttaca gcgccgaaac ccgccttcgt ggcgggtttt
     1561 tttacgcctg ccaacatgcc tagcctgccg cttcccgggc catgcggcag ccgattggag
     1621 cgcgggcagc ccgcagggcc gaggccccgt cgtcttgggg ccgagccgag cgactcggct
     1681 gccgcatggc ccgggaagcg gcagacgggt tatggaccca gggcttatga accaagcccc
     1741 tctataatcc agggtttaaa gcccgaatca cccacacgga ttccatgcag gaacgctacc
     1801 agcccaattc cgtcgaagcc gccgcccagc aggactggca ggcccgcaat gcctacctcg
     1861 tctccgagca cgtcaagaac gccgacggct ccgagaagcc caagttctac gcctgctcca
     1921 tgctgcccta ccccagcggc aagctgcaca tgggccatgt ccgcaactac accatcaacg
     1981 acatgatggc gcgccagttg cgcatgcgcg gctacaacgt gctcatgccc atgggctggg
     2041 acgccttcgg catgcccgcc gaaaacgccg ccatcaagtc caaggtgccg ccggccaaat
     2101 ggacctacga caacatcgcc tacatgaaga agcagatgct ggcgatgggc ctggccatcg
     2161 actggtcgcg cgagatgtgc gcctgcgatc ccaagtacta caagtggaac cagtggctgt
     2221 tcctcaagat gctcgaaaaa ggcatcgcct accgcaagac ccaggtcgtc aactgggacc
     2281 cggtcgacca gaccgtgctc gccaacgaac aggtcatcga tggcaaaggc tggcgctccg
     2341 gcgcgcccgt tgaaaaacgc gagatccccg gctactacct gcgcatcacc gactacgccg
     2401 acgagctgct gcgcgaagtg cagaccggcc tgcccggctg gcccgagcgc gtgcgcgtca
     2461 tgcaggaaaa ctggatcggc aaatccgagg gcctgcgctt cgccttcccg catcgaatcc
     2521 agggcgccga cggccggctc atccaggacg gccgcctgta cgtcttcacc acccgcgccg
     2581 acaccgtcat gggcgtgacc ttctgcgccg ttgcgccgga acaccccctg gccacgcacg
     2641 ccgcgcagtc cgacgccaag ctcgccgcct tcatcgagga atgcaagctg ggcggcacca
     2701 ccgaggccga actcgccacc cgcgaaaaat ccggcctgcc cacggggctg tcggtcaccc
     2761 accccatcac cggcgaacag atcccggtgt gggtgggcaa ctatgtgctc atcagctatg
     2821 gcgacggcgc cgtgatgggc gtgcccgccc acgacgagcg cgacttcgcc ttcgcccgca
     2881 agtacggcct ggccatcaag caggtcgtgg cgctggaagg caagacctat tccaccgatg
     2941 cctggcagga atggtacggc gacaagcagg ccggcaagac catcaactcg ggcaaatacg
     3001 acggcctcac gcataaagaa gccgtcgatg ccgtggccgc cgacctggcc ccgcagggcc
     3061 tgggcgaaaa gcagaccacc tggcgcctgc gcgactgggg catctcgcgc cagcgctact
     3121 ggggcacgcc catccccatc atccactgcg ccgactgcgg ccccgtgccg gtgcccgaga
     3181 aggacctgcc cgtggtcctg cccgaagacc tcatccccga cggcagcggc aacccgctgg
     3241 ccaagcacga ggccttcctg gcgtgcgcgt gccccaaatg cggcaagccc gcgcggcgcg
     3301 agaccgacac catggacacc ttcgtggact cgtcctggta cttcatgcgc tacacctcgc
     3361 ccggcaacga cggcgccatg gtcgatgccc gcaacgatta ctggatgccc atggaccagt
     3421 acatcggcgg catcgagcac gccgtgctgc acctgctgta cgcccgcttc tggaccaagg
     3481 tcatgcgcga catgggcatg ctcaagttcg acgagccctt caccaagctg ctgtgccagg
     3541 gcatggtgct caaccacatc tattcgcgcc gcaacgaaaa aggcggcatc gagtacttct
     3601 ggcctgaaga agtcgagaac ctctacgacg ccaagggtgt catcaccggc gcccagctca
     3661 agtccgatgg ctcgccggtg gactacggcg gcgtgggcac catgtccaaa tccaagaaca
     3721 acggcgtcga tccgcaggcc ctgatcgacc accagggcgc ggacaccgcc cgcctgttcg
     3781 tcatgttcgc cagcccgccc gagcagacgc tggagtggtc cgactccggc gtggacggcg
     3841 ccaaccgctt cctgcgccgc ctgtggtcgc tggcttatgc ccagcgcgag gccgtggcgc
     3901 gcggcctggc cgccggtttg gactggcagc aggcccccgc cccggtcaag gacctgcgcc
     3961 gcgaggtcta cggcctgctc aagcaagccg actacgacta ccagcgcatt cagtacaaca
     4021 ccgtggtgtc ggccggcatg aagatgctca acgccatcga atcggccagg ctgcccgccg
     4081 acggcaccgc gggggccgcc accgacgcgg cgctggccga gaccctgggc atcctgctgc
     4141 gcgtgctgta cccggtggtg ccgcacgtca cctggcagct gtggcgcgac ctgggctacg
     4201 cccacgaact gggcgacctg ctcgacgctc cctggccgca cgtcgacgaa gcggcgctcg
     4261 tggccgacga aatcgagctc atgctgcagg tcaacggcaa actgcgcggt tcgctgcgca
     4321 tgcccgccca ggccgccaag gaagacatcg agcagcaagc cgcagcccat cccgaaacgg
     4381 ctcgcttcct cgaaggccgt ccggccaagc gggtcatcgt cgtgcccggc aagctggtca
     4441 atgtcgtcgg ctagaggtcc attgatgaat cacgcctggt tggccctacg tacccgctcc
     4501 cgctggatgg tttccgctgc cggcctggcg ctgcttaccc tgctggccgc ctgcggcttc
     4561 cagatgcgcg gcgtgacgcc cttgccgttc gacacgctct atgtcggcgt gtccgacacc
     4621 accaagttcg gcgccgacct gcgccgcgcc ctgcgtgccg catcgcccgg cacgcggctg
     4681 gtcgactccc ccaaggacgc ccaggtcctg ctgcaacagg tctcgagcca gcgtttgatc
     4741 gacgacaccg cgctcaatgc ccagggccgc gtcgaagagt acgaactgag cgtgtatttc
     4801 accttccgcg tgatcgacgc gaagggcaac gccatcctgg gcgacaccgc gttctcggcc
     4861 ttccgcgagc tgccctacaa cgatcaggcc gcccaggccc tgagcagcca gatcgattcg
     4921 ctgtacgcct cgatggagca gtcgctcatc aaccgcgtgc tgcgccgcct caacgccatc
     4981 gacgtgcgcg aaaactacgc gcgcctgcag cgcggcgaag tcaatcccga ccgcccggta
     5041 ttcgatccga ccaaggtggc gccggtcccg gccatgcccc cgggctggaa ctctcccacc
     5101 aacaacctgg gcggcccgcc gcgctgagat gaaacggcct gcacgagtgc gttcgctgcc
     5161 cccccggcaa agctgacatg gccacggccc tggacgccag cagtctggcc agccacctgg
     5221 cgcgcaacgg ccagagcctg gcaccgctct atacggtgtc gggcgacgaa ccgctcctgg
     5281 tcatcgaggc catcgatgcc ctgcgcgccg ccgcgcggca ggccggctac gccgaccgca
     5341 cttcgctcgt catggacgcg cgcagcgact ggagcgcggc gcttgccgcc acgcagaccg
     5401 tctcgctctt cggcgaccgc agcctgctcg agatcaagct cgacagcggc aaacccggca
     5461 agaccggcgg cgagaccctg gtccgcctgg ccgaacaggc ccgggttcag gcccagaccc
     5521 aagccaatcc cgatgtcctg gtcatcatcg ccctgccgcg gctcgacagg gacacgcgca
     5581 acagcaaatg ggcgcaggcc ctggcggccg gcggcgtcat ggtcgacatc gccaccgtcg
     5641 accggccgca gctgccggcc tgggtcggtg cccgcctggc ccgccagaac cagcgcgccg
     5701 acaacgccac cctgcaatgg atggccgaca agatcgaagg caacctgctg gccgcccacc
     5761 aggaaatcca gaagctcggc ctgctctacc ccgaaggcga gctctccgcc gaggacgtgg
     5821 aacgcgccgt gctcaatgtg gcccgctacg acgtcttcgg cctgcgcgac gccatgctgg
     5881 ccggcgacag cgcgcgcacc ctgcgcatgc tcgaaggcct gcgcgccgag ggcgaagccg
     5941 aaccgctggt gctgtgggcc gtcggcgagg aaatccgcct gctgtcgcgc ctggccgacg
     6001 cccgccagca agggcaggac gtgggcggcc tgatgcgccg cctgcgcatc ttcggcaacc
     6061 acgaacgcct ggccttgcag gcgatttcgc gcgtgtcgcc caagtcctgg cccgccgccg
     6121 tgcagcatgc ccacgacgtc gaccgcctca tcaagggcct gcccacctcc ggccggatgt
     6181 ccgacgcctg ggaagaaatg gcccgcttgg ccctgcgcgt ggccgccgcg ggaacccggt
     6241 aaactaattt tcttttcgac gctccccctt ttgagctgct aagagccgag gaccccggag
     6301 caattcgaag cgcagccccc acccgggggc gagcatcgga attgcgcagg gcagcctcgg
     6361 cgcctcaaga actcaacagg gacgccgcat aaaaaaacca gaacatgtcc atagaacaaa
     6421 ccatgctggc tctgggccag cgcgcccgaa ccgcctcgcg gcacaccatg ctggccgata
     6481 ccggccagaa gaaccaggcc ctgctggcca tggccgatgc catcgccgaa caccgcggcg
     6541 aactgcaggc cgccaacgcg gccgacgtcg ccgacgccag gcgcgacggc ctcgacgccg
     6601 ccctgctcga ccggctcacg ctgtccgacc ggcacatcgg ccagatgcag gaaggcctgc
     6661 gccaggtcgc cgccctgccc gacccggtcg gcggcatcac ggcctcgacg ctgcgcccca
     6721 acggcatgcg cgtggcgcag atgcgcgtgc cgctgggcgt catcggcatc atctacgaat
     6781 cgcgccccaa cgtcaccatc gacgccgccg cgttgtgcct gaaatccggc aacgccgcca
     6841 tcctgcgcgg cggccgcgag gccctgcgct cgaacctggc cctggccaag gtggtgcgac
     6901 aaggactggc cgcggccggc ctgccgcccg aagccatcca ggtcgtggaa acgagcgacc
     6961 gcgcggccgt cggcgcactc atcaccatga ccgagtacat cgacgtgatc gtgccgcgcg
     7021 gcggcaaggg cctcatcgcc cgcctggcgc aggatgcccg cgtgccgctc atcaagcacc
     7081 tggacggcaa ctgccacgtc tacctcgaca gcgcggccga tcccgcccgc gcccacgaga
     7141 tcgcctacaa cgccaagacc tatcgctacg gcatctgcgg cgccatggaa accctgctgg
     7201 tgcacgccgc catcgccccg gccgtgctgc cgggcctggg ccgtgccctg cacgaccatg
     7261 gcgtcgagtt gcgcggctgc gcgcgcacgc gggcgctgct gccctacgcc gtcgcggcca
     7321 ccgaggaaga ctggggcacc gaatacctgg ggcccattct cgccatcaag atcgtcgaca
     7381 acgtcgatga agccatggcg cacatcgcgc gctggggctc gggccatacc gacgccatcg
     7441 tcaccgaaaa cctggccacg gcgcggcaat tccaacgcga ggtcgactcc agctcggtct
     7501 acgtcaacct gcccacctgc tttgccgacg gcttcgaata cggcctgggc gcggaaatcg
     7561 gcatctcgac caaccggctg cacgcgcgcg gccccgtcgg gctggaaggg ctcaccacgc
     7621 tgaagtgggt gctcgaaggc aacggacaga tccgagggta agacaccatg cagaatctcc
     7681 ccctggttcc ggttttccag ctgaccttca gcctcttcgt gctgtgcttt gccgcctgca
     7741 cggtctgcgg cctgctcttg ttctacttca agagcgacca ggtcaacaat gtcatgcagc
     7801 acccctacct gcagcacaag ccgttcaagc agctgccttt caccatccgc gccaccatcc
     7861 tcctcgacta ctacctgcac ctggtgtttc ccaagcgccg tttctgggtg atcgggcagg
     7921 ccaactacct gctccagcac gtcgatcccg cccaggtgcc caagggcgtc aagtggctga
     7981 tctgcagttt ctggggatcc tgctggctgg ggctgatcga catggcggtg ctctggggcg
     8041 tgctgtcgct gggcgcgggc ctgggcctct aggcctgcgc gaacgactcg acgatcgcat
     8101 cggccacggc gcgcacgcgc gccgtgcggt gcaggtcgga atgcatcacc agccagatgt
     8161 cgtaaccctc ctcgcgcctg ggccataggc gcaccagctg cgcaaactcc tgcgtcagat
     8221 ggttgggcag ctcgccgatg cccaggccgc tagccaccgc ctccatcatc aacagcccgg
     8281 aattgacctc cacggcgatc accgcattgt gcgtgggctc accgcagaac gtatccggcc
     8341 gctgcggcag caacccgcgc tcgtaagcca ccaatcgatg ccccgcgaac gcctcgccgg
     8401 gccgcggctc gccgtagcgc tgcagatagt cctgcgtggc gtacagccct accgtgcggc
     8461 gcgacaggtg gcgcacgatc agctcggggt tgtcgggccg caccacgcgc accgccaggt
     8521 cggcctcgcg gcgcgtcagg ttggtgacgt gcggcgaggt ggccagcacg acctggatgt
     8581 ccgggtgctg cgcctggacc tgcgcaatgg cctgcatcag gaaatgccgg cctatggtgt
     8641 ccgaggtggc cacccgcacc acgccgctca gccgccgatc cagcccctgc atctggcgcg
     8701 ccaactggtc ggccgcctgt tccatgcgtt cggccgcggc ataggccatt tctcccgccg
     8761 tggtcggcac gtagccgcgc ggcgtgcgca ggaagagccg cgcctccagc gaggcctcca
     8821 gcgccgccag gcggcgcccg accgtggcct ggtccacctg cagcgcgacc gccgcggctc
     8881 tcagggtgcc gctgcggtaa agcgccagaa aagtgcgggc gttgtcccaa tccacgatcg
     8941 atgcgcatcc ctttcgacgc tgtgcgccgt atgatgcatt tttgcatgca taggcggcaa
     9001 ttctagtgct ttattgcatc gcccagccgg cttacgatgc tgtcttgctc cttgccagat
     9061 ccgtcatcca caccatgtct tcctcttgcc ccgccatccc ggccagcccc gccctgccgg
     9121 cccacgatcc cagcctgctg ccgctggcca cgctggccat cggcttcgtc atggccatgc
     9181 tcgacgtcac ggtcgtcaac gtggccctgc ccagcatcgc cagccaattc aacaccccgc
     9241 tgaccaacct ggtctggatc gtcgacggct acaccctcac tttcgctgcc ctgctgctcg
     9301 tcgccggcgc cctggccaat cgtttcggcg ccaagcgcat ctacctgacc ggactggccg
     9361 tcttcacgct ggcctcgctg ctgtgcgggc tgtcgctcaa tgccggcatg ctgatcggcg
     9421 cgcgcctggt ccagggcttc ggcgccgcgc tgttcatgcc cagttccctc agcctgctcg
     9481 cgcatgccta cgacaacgac accgtgcgca cccgcatgct gtcgctctgg tcggccatcg
     9541 tctcgatcgc cgccgcggcc gggccgctgg tcggcggcct gctgatcgac acattcggct
     9601 ggcgcagcgt cttcctggtc aacatccccg tgggcctgat cggactttgg ctggccagct
     9661 cccgcattgc cgccgcgccc cgccaccccc acccgctcag catcaccagc cacgcgctgg
     9721 gtgtgctgtt cctgggttcg atctgctacg ccct
//
LOCUS       DASWPO010000157         4811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2535047, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000157 DASWPO010000000
VERSION     DASWPO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535047"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VGC69_08680"
     CDS             complement(<1..137)
                     /locus_tag="VGC69_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY0295388.1"
                     /translation="MLHISGALYLDERDIVFSMIRAQGAGGQNVNKVSSAVHLRFDVR
                     AS"
     gene            291..932
                     /gene="queE"
                     /locus_tag="VGC69_08685"
     CDS             291..932
                     /gene="queE"
                     /locus_tag="VGC69_08685"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY0295389.1"
                     /translation="MAYSVKELFKTLQGEGAQAGRAAVFCRFAGCNLWSGRESDRAQA
                     VCTFCDTDFVGTDGAGGGKFANAQALARAIAACWGDGADKRYVVFTGGEPLLQLDDAL
                     VAAVHAQGFAIAIETNGTLAPPEGIDWICVSPKGTSQLAITRGNELKLVYPQLDALPE
                     RYTHLAFEYFFLQPMDGPGETARAANTERIVRYCLQHPQWRLSLQTHKYIGIP"
     gene            929..1402
                     /gene="queD"
                     /locus_tag="VGC69_08690"
     CDS             929..1402
                     /gene="queD"
                     /locus_tag="VGC69_08690"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY0295390.1"
                     /translation="MNHPHAEPGPALSVTRRLEFDAGHRIPDHRSQCRNLHGHRYVLE
                     VTLSGDIVSTPGASDNGMLMDFSEIKAIAQAHIVEVWDHAFLVYEGDVPVYDFLQSLP
                     GHKTVALDRIPTVENLAAIAFETLAPHYQGHYGNNLRLTRVRLYETPNCWADCEG"
     gene            1503..1970
                     /locus_tag="VGC69_08695"
     CDS             1503..1970
                     /locus_tag="VGC69_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0295391.1"
                     /translation="MKHPTDETRQRVMSTLMVLSRAYRGAVDKALASYGLSQATAWPV
                     IMIARRGDGVRQGVLAEALGVEGPSLVRVLDHLGTAGLIERREDAHDRRSRTLHLTEA
                     GRDLYGQIESVIVELRRDLLKDASAEDLEACLRVFDSLKTTLGREHAAPPQDG"
     gene            1973..4006
                     /locus_tag="VGC69_08700"
     CDS             1973..4006
                     /locus_tag="VGC69_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0295392.1"
                     /translation="MKLPTATETVFAVKCYAAAMAALYLAYSIGLTRPFWAMTTVYIV
                     SQPLAGAVRSKAVFRLVGTFVGATVAIATIPTLSNYPVLVVAFLSLWIGLCLYFGVLD
                     RTPRSYLFMLAGYTVSLVGLSSIAAPGDIFDLALARVEEISLGILCATLVHSLILPRG
                     LAQAVIARLDATVADARTWMQIVLGGDTGGKTAKERHVLANDISLLRQLSTHVPFDTS
                     NIRWTVQPLNLIQDQIAAITAVVSAIEDRLLALREAGHELPAPVAQALTDVSAWLAQG
                     RKADPAQAGELRRRLAGLMPVIEPGLHWEDALLVSLLARLRELIHTFRRCVDLRQDIQ
                     ASLEKARARAPRRPRARGARLHGDHPLALWSAITTTLTIATCCTVWILTAWPYGSTAA
                     IMASVFGALYSSLDNPAASIKNFMKFILASMPVSALYLLVVMPMIDTFDMMALSFFPA
                     LFVMGVLMARPAYTAKATPIVIGFCGTLALYDYTSVSNQVAFFEFSVSQAMGLGIATI
                     IAAIFRNARPAWRARRIQAANWNELARLASSSGSRAGHVYATRMLDRVSLLQMRLAAA
                     DRAASDTLLDLRVGRDITELQRARRRLPAAETAIGGVLQGLARYYRGRSAGRHAQPPE
                     LLAQIDATLAGIVTHGGLSQARTRATVALVGIRRDLYPGTVSPAGIGLQASPA"
     gene            4003..4215
                     /locus_tag="VGC69_08705"
     CDS             4003..4215
                     /locus_tag="VGC69_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEY0295393.1"
                     /translation="MIGELDLYGAYVPWLLVLGLCAWVLFWLAQKLLARLGAYRLVWH
                     PPLFNLALYVIVLYGVMRLSPWIFQR"
     gene            4220..>4811
                     /locus_tag="VGC69_08710"
     CDS             4220..>4811
                     /locus_tag="VGC69_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0295394.1"
                     /translation="MKLPETLKPEALGRYAVTALVVLAALYAGWRIWQHYELDPWTRD
                     GHVRADVVQVAPDVSGLVTAMLVHDNQDVHAGDVLFEIDRARFQLALDQAQAALTARQ
                     VARDQAARDVKRNQGLGQLVSAEALEQSRTRLEQSEADIAQAVVQVHTAQLNLKRSQV
                     LASASGRVTNLDLHVGSYANAGRPLMALIDANSFYVE"
BASE COUNT          755 a         1819 c         1474 g          763 t
ORIGIN      
        1 gaggcgcgca cgtcgaagcg caggtggacg gcgctggaaa ccttgttgac gttctgcccg
       61 cccgccccct gggcgcggat catggagaac acgatgtcgc gctcgtcgag gtagagagcg
      121 ccggagatgt gcagcagcat aagtccgcaa tctacccgat cccgcccggg acgcaagacc
      181 ggcccggcca gggccgggag cgggggccgg acgaggcgcc cgcaaatccc cggtaaaata
      241 gcggattccc gatattttcc ttgcgcaccg gccccggctg gcggcgtccc atggcttatt
      301 ccgtcaaaga actcttcaaa acgctgcagg gcgaaggcgc ccaggccggc cgcgcggccg
      361 tgttctgccg cttcgccggc tgcaacctct ggagcggccg cgaaagcgac cgggcgcagg
      421 ccgtctgcac cttctgcgac accgacttcg tcggcaccga cggcgcaggc ggcggcaagt
      481 tcgccaatgc ccaggcactg gcccgcgcca tcgccgcctg ctggggcgac ggcgccgaca
      541 agcgctacgt ggtcttcacc ggcggcgagc cgctcctgca gctcgacgac gcgctggtgg
      601 ccgccgtgca tgcgcaaggc tttgccatcg ccatcgaaac caacggcacg ctcgcgccgc
      661 ccgagggcat agactggatc tgcgtcagcc ccaagggcac ctcgcagctg gcgatcacgc
      721 gcggcaacga gctcaagctg gtctacccgc agctcgacgc cctgcccgaa cgctacacgc
      781 acctggcgtt cgagtacttc ttcctgcagc ccatggacgg ccccggggaa acggccaggg
      841 cggccaacac cgaacgcatc gtgcgctact gcctgcagca cccgcaatgg cggctgagcc
      901 tgcagactca taaatacata ggcattccat gaatcaccct catgccgagc cgggcccggc
      961 cctgtccgtc acccgccggc tggaattcga cgcgggccac cgcatccccg atcaccgcag
     1021 ccagtgccgc aatctgcacg gccaccgcta cgtgctcgaa gtcacgctca gcggcgacat
     1081 cgtgagcacg cccggcgcgt ccgacaacgg catgctgatg gacttctccg agatcaaggc
     1141 cattgcccag gcccatatcg tcgaggtctg ggaccatgcc ttcctggtgt atgaaggcga
     1201 tgtgccggtg tacgacttcc tgcagtcgct gccgggccac aagacggtcg cactcgaccg
     1261 cattcccacc gtcgagaacc tggcggccat cgccttcgag accctggccc cccattacca
     1321 gggccactac ggcaacaacc tgcgcctgac ccgggtgcgc ctgtacgaaa cccccaactg
     1381 ctgggccgac tgcgaaggct gacaggcccg tcccctttca ttgcacggcg tttttttgcg
     1441 atttcgtcgt tttttagcat cctaatagat agctttctaa taattaggat acaatcttct
     1501 tcatgaaaca tcccacggac gaaaccagac aacgcgtcat gagcacgctg atggtgctgt
     1561 cgcgcgccta tcgcggcgcc gtcgataagg cgctggccag ctatgggctg tcgcaggcca
     1621 cggcctggcc cgtcatcatg atcgcccgcc gcggcgacgg cgtgcgccag ggcgtgctgg
     1681 ccgaggccct gggcgtcgag gggccgtcgc tggtgcgcgt gctcgaccac ctgggcaccg
     1741 ccggcctgat cgagcgccgc gaggacgccc acgaccggcg cagccgcacc ctgcacctca
     1801 ccgaggccgg ccgcgatctg tacgggcaga tagaaagcgt gatcgtcgaa ctgcgccgcg
     1861 acctgctcaa ggacgccagc gccgaagacc tcgaagcctg cctgcgagtc ttcgacagcc
     1921 tcaagacgac gctgggccgg gaacacgccg cgccgcccca agacgggtag ccatgaaact
     1981 gcccaccgcc actgaaaccg tctttgcggt caaatgctat gcggccgcca tggcggccct
     2041 ctacctggcc tattccatcg gcctgacgcg cccgttctgg gccatgacca ccgtctacat
     2101 cgtctcccag ccgctggccg gcgccgtgcg ctccaaggcg gtcttccgcc tggtcggcac
     2161 cttcgtcggc gcgaccgtcg ccatcgccac gatacccacc ctgtccaact accccgtgct
     2221 ggtggtggcc ttcctgtcgc tgtggatcgg cttgtgcctg tatttcggcg tgctcgaccg
     2281 cacgccgcgt tcgtacctgt tcatgctcgc cggctacacg gtctccctgg tcgggctctc
     2341 cagcatcgcc gcgccgggcg acatcttcga cctggccctg gcgcgcgtgg aagaaatcag
     2401 cctgggcatc ctgtgcgcca ccctggtcca cagcctcatc ctgccgcgcg gcctcgcgca
     2461 ggccgtcatc gcgcggctgg acgccacggt cgcggacgcc cgcacctgga tgcagatcgt
     2521 gctgggcggc gacaccggcg gcaagaccgc caaagagcgc cacgtcctgg ccaatgacat
     2581 ctcgctgctg cgccagctgt ccacccatgt gccgttcgac acctccaaca tccgctggac
     2641 ggtccagccc ctgaacctca tccaggacca gatcgcggcc atcaccgccg tggtctcggc
     2701 catcgaagac cgcctgctcg ccctgcgcga ggccggccac gaattgcccg cgcccgtggc
     2761 gcaagcgctg accgacgtct cggcctggct ggcgcaaggc cgcaaggccg accccgccca
     2821 ggccggcgaa ctgcgccgcc gcctggccgg gctcatgccc gtcatcgagc ccggcctgca
     2881 ctgggaggac gccctgctcg tcagcctgct ggcccgcctg cgcgagctca tccatacctt
     2941 ccggcgctgc gtggacctgc gccaggacat ccaggccagc ctggaaaaag cccgggcacg
     3001 cgcgccgcgg cgcccgcgcg cgcgcggcgc gcgcctgcac ggcgaccatc cgctggccct
     3061 gtggtcggcg atcaccacca cgctgaccat cgccacctgc tgcacggtct ggatactgac
     3121 cgcgtggccc tacggctcca ccgccgccat catggcctcg gtcttcggcg ccttgtactc
     3181 ctcgctggac aacccggccg cgagcatcaa gaacttcatg aagttcatcc tggcctcgat
     3241 gccggtctcc gcgctgtacc tgctcgtcgt catgcccatg atcgatacct tcgacatgat
     3301 ggccctgtcg ttctttcccg cgctcttcgt gatgggcgtg ctgatggccc gccccgcgta
     3361 caccgccaag gccaccccca tcgtgatcgg cttttgcggc acgctggcgc tctacgacta
     3421 caccagcgta agcaaccagg tcgccttctt cgagttcagc gtgtcgcagg ccatgggcct
     3481 gggcatcgcc acgatcatcg ccgccatctt ccgcaacgcc cggcccgcct ggcgcgcccg
     3541 gcgcatccag gcagccaact ggaacgagct tgccaggctg gcaagctcct ccggcagccg
     3601 ggccggacac gtctatgcca cgcgcatgct cgaccgcgtc agcctgctgc agatgcgcct
     3661 ggccgcggcc gaccgggccg cctccgacac gctgctggac ctgcgcgtcg gccgcgacat
     3721 caccgaactg cagcgcgcgc gccggcgcct gcccgcggcc gagaccgcca tcggcggcgt
     3781 gctgcaaggc ctggcgcgct actaccgcgg ccgttcggcg gggcgccacg cgcagccgcc
     3841 cgagctgctg gcgcagatcg acgccacgct ggccggcatc gtcacgcacg gcggcctgtc
     3901 ccaggcgcgc acccgcgcca cggtggccct ggtcggtatc cgccgcgacc tctatcccgg
     3961 caccgtcagc cccgcaggta tcggcctgca ggcgtccccc gcatgatcgg cgaactcgac
     4021 ctctacggcg cctacgtccc ctggctgctg gtcctgggcc tgtgcgcctg ggtgctcttc
     4081 tggctggcgc agaagctcct ggccaggctg ggcgcctacc ggctggtctg gcacccgccg
     4141 cttttcaacc tggcgctcta cgtcattgtt ctctatggcg tgatgcgcct gtccccttgg
     4201 attttccaga gatagcagca tgaaactccc cgagaccctc aagcccgagg ccctgggccg
     4261 gtacgccgtc acggctctcg tcgtgctggc cgcgctctat gccggctggc gcatctggca
     4321 gcactacgag ctcgacccct ggacgcgcga cggccacgtg cgcgccgacg tcgtccaggt
     4381 agcgcccgac gtatccggac tggtgaccgc catgctggtg cacgacaacc aggacgtcca
     4441 tgccggcgac gtgctgttcg agatcgaccg cgcccgcttc cagctggcgc tggaccaggc
     4501 gcaggccgcg ctgaccgccc gccaggtggc gcgcgaccag gccgcgcgcg acgtcaagcg
     4561 caaccagggc ctgggccaat tggtgtcggc cgaagcgctg gagcaaagcc gcacccgcct
     4621 cgaacagagc gaagccgaca tcgcccaggc cgtggtgcag gtgcacaccg cgcagctcaa
     4681 cctcaagcgc agccaggtgc tggcctcggc ctccggccgc gtcaccaacc tcgacctgca
     4741 cgtgggttcg tacgccaacg ccggtcgccc cttgatggcc ctgatcgacg ccaactcgtt
     4801 ctatgtcgaa g
//
LOCUS       DASWPO010000158        11248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_838848, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000158 DASWPO010000000
VERSION     DASWPO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838848"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VGC69_08715"
     CDS             complement(<1..655)
                     /locus_tag="VGC69_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HEY0295395.1"
                     /translation="MTIKKRGQIAVLAVVVAVLALVAVQAIWRPLQRRVVLRSHPAAL
                     DVGYPDAIIDSGSLSRLPADIIRVPLLHSLLTRDFVDYYESNPTRMSVEGTLRRLAFE
                     HDLPWRDELLKRVFDEPSRVLLWRTPDGRLGYWLMSMRRNGFAKLLQAVGNVVSDDTQ
                     LSQAGSLPGDVPLYALKLAVGRTLLFASKGDQLVVLSSPQLLMDQQGQLIQDRARMVA
                     "
     gene            1015..3864
                     /locus_tag="VGC69_08720"
     CDS             1015..3864
                     /locus_tag="VGC69_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEY0295396.1"
                     /translation="MRWFIGILAFVGGALFALNDDLPVYECAIAFAVVGFVLTTLVGR
                     SARQAQGASAPEIQAPRTEADPEFLAVRISRLEQELAQIRLELSQRRPLSSDAQPPSM
                     PVAPDTGALGATTADARAVTATAQATAASSMADAMQANAAPPDTDNIPATDQPPLPAA
                     VRQPGILERGLAATRDWFLGGNTVVRVGIVVLFFGIAFLLKYAADNDIFPIEFRLAGV
                     ALCAIALLVTGWRLRGRRQVYGLVLQGGAVGMLYLVIFAATRLYGLLPSNGALPLMIA
                     VCALSAILAIRQDAPALAFMGSAGGFLAPILLSTGGGSHIMLFGYYALLNAGILGIAW
                     FKAWRPLNLLGFVCTFGIGAAWGATAYQPELFASAESFLLLFFLMYVGVALLYAIRRD
                     LALTHYVDGTLVFGTPLLASLMQARLVYDLPFGLAYSAVALAAFYLGLAVWLRRSHPR
                     LNLLGHTMLALAIIFMTLAIPAAFSGPTTTAVWALEGAAAVWINLRQRRLIALIFGLL
                     LQLGAAFVFLGQAGSDVEAAARMPVLNGEYLATLFIGLAALFSGWRLHGKRESGAWLP
                     WLPQVGIAAAAWGLLWWLGGGLEEIQAFHILHPDMPFEGIYDAVVLFILLTAWLLWGA
                     QRVLNWPLAKWPAAMLAPALAVLSVVISMDIPPLSEVPALPVALALSYLLLHKEERDD
                     LPPRLLDALHTLSFWTLCGLLTVQAFYQMGAWVPEGAWQWSAWAYGYGLALLLLVGPG
                     AGLAWPVQQHSRAYLVWGAAPLALLLWAWSLASVVNDGDASPLFYLPILNPLDVAQLL
                     AALAVANWMKRLGSTTSCLAQYGAKLRAAAALTGFIWLNAVLLRTLHHWSGVDYRLEA
                     LGASTLVQASVSVFWTICALATMIMATRRAGRTPWFAGACLLAITIVKLFLFDLSYLA
                     GVSRIVAFIGIGLLLLLIGYFSPLPPKQAATEAHS"
     gene            3861..5195
                     /locus_tag="VGC69_08725"
     CDS             3861..5195
                     /locus_tag="VGC69_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HEY0295397.1"
                     /translation="MRTERILARICLLGSLILSIEAPAYSEPPSPGAQRFALELPSAS
                     AYYQVTLTEAAIAASLRDLDDLRVFNGAGEPVPYTLEGAATPAQQAAVQDLAWFPLPA
                     DATASATDLGVTIEPDGRLRAIARPSSPATDRGGDLVDLGPGATPLSALLISLSNDTY
                     LGRVDISASDDLRQWTPVTQAQLLKTHNGAAILVQKRVELDGVRARYLRLKWPDGAPA
                     IAALQAESVPDSPPPARQWRTGITMRPSGSPGEYRFDSGGAYPVDRVQFELPQPNTVA
                     RGQLYSRVDGSTPWRLVAEGQLLRLQATSPANGEQHNPPWSLDVDTDRQWLLRVDTRG
                     GGLGGGTAKAILGWQPVSLTFAARGPGPFVLEAGDPSWHAASVSREELLPPGSAAPAQ
                     AQVGQPLGQAPAQVPTDPQARRRYVLWAVLVAAVAMLGIMAWRLMRPRGPQA"
     gene            complement(5280..6053)
                     /locus_tag="VGC69_08730"
     CDS             complement(5280..6053)
                     /locus_tag="VGC69_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295398.1"
                     /translation="MLFATATLSGCVTTGTNGTAVSGSAGGESSVGASNTLEHCDSPL
                     GTLAMDDGRNADWYGQFGSTTHVTSIEPLLRLAVQQSNCFVITSIGNLRTDSRLSRIT
                     NMQRESGEYRAGSKQQKGQRVAADYYMEPQIIIDNDSVGGLGAAVGSMAGGIFGAVAG
                     TLQSKASVVTLTLYDIRSAVQISASEGSATATNFGAAFGALGGTAAGGLGGFSRTPEG
                     KATVAAFMDAYNGMVVALRNYKAQDVKGGLGRGGRLKVN"
     gene            complement(6171..6746)
                     /locus_tag="VGC69_08735"
     CDS             complement(6171..6746)
                     /locus_tag="VGC69_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEY0295399.1"
                     /translation="MKKIHPAALIICFLLASCAQSNQADTVRVCNSQGCSDRSRQAVS
                     QELNDIPDDGRIAALENIAKQTPRAAYDLGLRYFRGDGVPQDSYKAITWMRAAAEQGE
                     LEAQKALGRVYLTGLEEMGPDSREAQKWLSIASSRGDAEASKLLAQAEAARRSDEAEW
                     KWRQHWRGIFYNYWYSGYPYFGRWQGTYWIY"
     gene            7450..7578
                     /locus_tag="VGC69_08740"
     CDS             7450..7578
                     /locus_tag="VGC69_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEY0295400.1"
                     /translation="MNNKSFVLIIALLASLVAGCNTIHGAGKDIERGGEKIQEKSQ"
     gene            7952..8848
                     /locus_tag="VGC69_08745"
     CDS             7952..8848
                     /locus_tag="VGC69_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295401.1"
                     /translation="MQNAQPTTSYTHTSNGAESSRSAVSWSAVIAGAVIAAAVSAMLM
                     TGGSGLGFLSMSPWRDEGASGSALAIGSIVWLFVTQIIAYGVAGYVAGRLRTKWTDTH
                     DDEVYFRDTAHGFLVWALSAVVGLVLLGSTIASVVSGTAKAGATLAGAGAGAATAVAG
                     QAAQAGADHLSLDYFTDALLRPNDPAASNGPGDVRQEASRILARSLANGNVSDEDQSY
                     LVKVIAQRAGVDEAAAKQRLTQVEEQAKQAAQQAGQKAREAADAARKAAAAFTLWAFA
                     SLLVGAFVASFCATIGGRARDR"
     gene            8946..9992
                     /locus_tag="VGC69_08750"
     CDS             8946..9992
                     /locus_tag="VGC69_08750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0295402.1"
                     /translation="MKDPSSLSSLSDPAALRVFLGRQGTGADIYQVLQSLVEAGYGDL
                     PRPGSSRTLRRWQMLAAAAQFDLSLAKLYESHADALAILHELGHADLSMRGGIWGVWC
                     AEPPSHRVQACPRDSGGKSVRLHGAKAWCSGASHVTQALVSAWNDQGHSCLVALDLDQ
                     PEIEIVHEAWGAVGMAATASVDIVFHGAAGTLVGAPGAYLDRPGFYHGAAGVAACWYG
                     AASAIAQRVLDSVRSHQDAPHSLAHLGALDLVLTQARHQLRIAAAEIDANPGESCQQA
                     VRRARLAAESAAETILIRAPRAFGPGPMCQDVRIARLLADLPVFIRQSHAERDLQTHG
                     QALAEQATEALWTL"
     gene            9980..10738
                     /locus_tag="VGC69_08755"
     CDS             9980..10738
                     /locus_tag="VGC69_08755"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY0295403.1"
                     /translation="MDAVSTRAIAGAGTPEACWRAWLSHYPPARGAVEDLVLARHRLH
                     ILAPHPDDEVLGCAGIMRQALRLGRPVHVWAITDGEASHAGSRAIRASELAQVRTRES
                     ERALRRLGTGIRRHRLNFPDGGVGRHIPEIAGMLAPHVQPGDTIISPWSLDGHPDHEA
                     AACAGRQAARAHGCRLFEVVIWGWHWADPQRGEFPIQRAVAIALTAADCLAKARAIQA
                     FRSQLEPDPATGNPPILPDFVLARFQRPFEVVLR"
     gene            10735..>11248
                     /locus_tag="VGC69_08760"
     CDS             10735..>11248
                     /locus_tag="VGC69_08760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0295404.1"
                     /translation="MTDLDSHFDALYQASADPWSVTTAWYERRKRATLMAALPRERYR
                     HALELGCGNGATTRLLAERCDAVRAVDSSPAAVARCAHAVAAGGFTNVQLDVCRLPDT
                     WPLQQGEPVDLIVVSELAYYFTDADLDRLLSRCRQALAQDGDWLMCHYTRPFHDRLQD
                     TARIHDKINNL"
BASE COUNT         2006 a         3625 c         3456 g         2161 t
ORIGIN      
        1 tcgcgaccat ccgggcgcgg tcctggatga gttggccttg ctggtccatc agcaactgcg
       61 gcgaggacag cacgaccagt tgatcgccct tggacgcgaa caacaaggtg cgccccaccg
      121 ccagcttcaa ggcatagagc ggcacatcgc caggcaggct gccagcctgg ctcagttgcg
      181 tatcgtcgct taccacgttg cccacggcct gcagcagctt ggcaaagccg ttgcggcgca
      241 tcgacatcag ccaataaccc agccggccat ccggcgtgcg ccacaacaac acgcgcgagg
      301 gttcgtcgaa aacccgcttg agcaactcgt cgcgccacgg taggtcgtgc tcgaacgcca
      361 gccgccgcaa cgtgccttcg accgacatgc gggtcgggtt cgactcgtaa taatcgacga
      421 agtcgcgcgt caacaggcta tgcagcaaag gcacccggat aatgtcggcc ggcaaccggg
      481 acaaagagcc actgtcgata atggcatccg gatatccgac gtcgagcgcc gccggatgcg
      541 agcgcagaac cacccgtctc tgcaggggac gccatatcgc ctgcacggca accagcgcca
      601 aaaccgccac cacaacggcc aacaccgcga tttggccgcg tttttttatt gtcatgacta
      661 acgtctctac ccgactaatt ggttttctgc cgccccatca acccggcgat caaaacacta
      721 tgcgcgaccg gcgtgcgaac gcttattcag gttcagaatc ctacccgaac tcgctcctag
      781 caagcccagt tcggaaaatt tttactcttg caccactgcg aaatcaaaag aaacatccta
      841 ttaactttgg tacagacgct tctgatcggc catgacgctg tgttcgacat ttgtcggcac
      901 atcctaaacg ctcgaccagg cattgcaggc gctcaggcca gggacgtgtc aaccacgccc
      961 ctggcgagtt ggcataattg cgaccgggca cggcactcag actgagaggg aagcatgcgt
     1021 tggtttatcg ggattctggc gttcgttggc ggcgcgctgt ttgcgctgaa cgacgattta
     1081 ccagtctacg aatgcgcaat tgcattcgcg gtggtcggct tcgtgctgac aacgctcgta
     1141 ggccgttcgg cccgccaggc gcaaggcgca tccgcgcccg agattcaagc tccccgtaca
     1201 gaggcggacc cggagttctt ggctgtacgc atatcacgcc tcgagcagga gctggcgcag
     1261 atacgcctgg aactctcgca acgacgcccc ctatcatccg acgcccagcc gccttcgatg
     1321 ccggtcgcgc cggacaccgg ggcattgggc gccacgactg ccgatgcgcg cgctgtgact
     1381 gccaccgcac aggccacggc agccagctcg atggccgacg ccatgcaggc gaatgccgcg
     1441 ccgccagaca cggacaacat tcccgccacg gaccagccgc ccctgcctgc cgcggtgcgc
     1501 cagccaggca tcctggagcg cgggctggcc gccacgcgcg actggttcct gggcggcaat
     1561 accgtggtcc gggttggcat tgtcgtgctt tttttcggca tcgctttctt gctgaaatat
     1621 gcagccgata acgacatatt cccgatcgag tttcgcctgg ccggggtagc cttgtgcgcg
     1681 atcgcgctgc tggtcaccgg ctggcggctg cgcggccgcc gccaggtcta tggcctggtt
     1741 ctgcaaggcg gcgcggtcgg catgctctac ctggtcatct tcgccgcgac ccgcctgtac
     1801 gggctattgc cgtcgaacgg cgcattgccg ttgatgatcg ccgtctgcgc cctgagcgca
     1861 atactggcca tacgccagga tgcgccggcg ctggccttca tgggaagcgc cggcggattt
     1921 ctggcgccta tcctgctgag cacaggcggc ggcagtcaca tcatgctatt tggctattac
     1981 gcgctgctca atgccggcat cctgggcatc gcctggttca aggcctggcg gccgctcaac
     2041 ctgctgggct ttgtctgcac gttcggaatc ggagcagctt ggggagccac cgcctaccag
     2101 ccggaactgt ttgcttccgc ggagtcattc ctgctcctgt tcttcctgat gtacgtgggc
     2161 gtcgcgctgc tgtatgccat acgccgcgac ctggcgctga cgcattatgt ggacggcacg
     2221 ctggtgttcg gcacgccatt gctggcaagc ttgatgcagg cccggctggt gtatgacttg
     2281 cctttcggcc tggcctacag cgcggtggcg ttggcggcct tttatctggg gctggccgtg
     2341 tggctgcgcc gcagccatcc acggctgaac ttgctaggcc acaccatgct ggcgctggcc
     2401 atcatcttca tgacgctggc gattccagct gccttcagcg gtccgacgac cacggcggta
     2461 tgggcgctgg aaggtgcggc cgcggtctgg atcaacctgc ggcagcgcag gctgatcgcg
     2521 ctgatcttcg ggctgttgct gcaactgggc gcggcattcg ttttcctggg ccaggctgga
     2581 tccgatgtcg aagcggccgc ccggatgcca gtactcaacg gcgaatacct ggctacgctg
     2641 ttcatcgggc tggctgcctt gtttagcggc tggcgcttgc acggcaagcg cgaatcgggg
     2701 gcctggctac cgtggctgcc gcaggtcggc atcgccgcgg cggcctgggg actgctctgg
     2761 tggctggggg gcggcctgga ggagatccag gccttccata ttctgcaccc cgacatgccg
     2821 ttcgagggaa tctacgatgc ggtcgtcctg ttcatcctgc tcacggcctg gctgctgtgg
     2881 ggcgcgcagc gtgtgttgaa ctggccgttg gccaagtggc ccgcggccat gctcgcgccg
     2941 gcgctggcgg tcctgtccgt ggtgatttca atggacatcc cgcctttaag cgaggttccg
     3001 gccctgcccg tcgcgctcgc gttgtcctac ctgctgctcc acaaggagga gcgcgacgac
     3061 ttgcccccac gcttgctgga cgcgctgcac accttgtcct tctggacttt gtgcgggctg
     3121 ctgaccgtcc aggccttcta ccagatgggc gcctgggtgc ccgagggcgc gtggcaatgg
     3181 agcgcctggg cttacggata tggtctggcg ctcctgctgc tcgtcggccc cggcgcaggc
     3241 ctggcctggc cggtgcagca gcactcccgg gcctatctgg tctggggcgc cgcgccgctg
     3301 gcgctgttgc tatgggcatg gagcctcgcc agcgtggtca acgacggcga tgcctcgccg
     3361 cttttttacc tgccgatact caatccactc gacgtggcgc agctgcttgc ggcgctggca
     3421 gtggccaact ggatgaagcg gctgggctcg acgacgtcct gtctcgctca atacggcgcg
     3481 aagctgcggg cggcggcagc gctcaccgga ttcatctggc tcaatgcggt cctgctgcgt
     3541 acgcttcatc actggagcgg cgtggactat cgcctggagg cgctcggcgc ctcgaccctg
     3601 gtgcaggcgt cggtttcggt attctggacg atctgcgcgc tggccacgat gatcatggcc
     3661 acacggcgtg caggccgtac gccttggttc gccggcgcat gcctgcttgc gattactatc
     3721 gtcaagctct ttctgttcga tctttcgtat ctggcgggcg tgtcgcgcat cgtcgccttc
     3781 atcgggatcg gtctgttgct gctgctcatt ggctatttct ctccgctgcc gcccaaacaa
     3841 gcggcgacgg aggctcactc atgagaaccg aacgcatcct cgcgagaatc tgcctgctag
     3901 gttctctcat cctatcgatc gaagcgccgg catacagcga gcccccttcg cctggagcgc
     3961 aacgcttcgc actggaattg ccgtccgcat cggcctacta tcaggtcacg ctgacagagg
     4021 ctgccatcgc agcaagccta cgcgacctgg acgacctgcg tgtctttaac ggcgcgggcg
     4081 agcccgtgcc gtataccctg gaaggcgccg ccacacccgc gcaacaagcg gccgtgcagg
     4141 atctggcctg gtttccgctg cccgccgacg cgacagccag cgcaaccgat ctcggcgtga
     4201 cgatcgaacc ggacgggcgc ctgcgcgcca tcgcccgccc atcatctccg gcaacagatc
     4261 ggggcggcga tctcgtcgac ctgggccccg gcgcgacgcc attgtcggcg ctgttgattt
     4321 cgttgagcaa cgacacctac ttggggcggg tcgatatctc cgccagcgac gacctgcgcc
     4381 aatggactcc ggtaacgcaa gcccagttgc tgaaaacgca caacggtgcg gcgatcctcg
     4441 tgcagaagcg cgtggagctc gacggcgtgc gggcccgcta cttgcggctg aaatggccgg
     4501 atggcgcccc ggccatcgcc gcgctgcagg cggaatccgt tcccgacagc ccgccgcctg
     4561 cccggcaatg gcgaaccggc atcaccatgc ggccaagcgg ctcaccgggt gaataccgct
     4621 tcgacagcgg cggcgcctac cccgtagacc gggttcagtt cgaactgcca cagcccaata
     4681 ccgtcgcgcg cggacagctt tattcacgcg tcgatggcag caccccctgg cgcttggtgg
     4741 ccgaaggcca gctgctgcgt ctccaagcga ccagcccggc aaacggcgag caacacaacc
     4801 cgccctggtc cctggacgtc gacaccgacc gccaatggct cctgcgggtg gacacgcgcg
     4861 gcggcggatt gggcggcggc actgccaagg ccatcctggg ctggcagccc gtgtcgttga
     4921 cattcgccgc gcgcgggccg ggacctttcg tgctcgaggc aggagatcca tcctggcacg
     4981 cggcgtcggt ttcacgcgaa gagctcttgc cgccaggctc ggcggcaccg gcgcaggccc
     5041 aggttggcca gcccttgggc caagcgcccg cccaggtgcc caccgatccg caggcgcggc
     5101 gccgatacgt gctatgggcc gtgctggtcg ccgcggtggc aatgctcgga atcatggcgt
     5161 ggcggctgat gcgccctcgg gggccgcagg cgtgatctcc acagagaccc gtccgcggcg
     5221 cgcccgatcc gcgttctgcc agcagggagc gggcctaccc taacgcaggt aactcagtct
     5281 taattgacct tgaggcgacc gccgcgacca aggcctcctt tgacatcttg tgctttgtag
     5341 ttccgcaaag cgacaaccat gccgttgtag gcatccatga acgcagcaac agtggctttc
     5401 ccttcgggtg tccgggaaaa gccgcccagc ccgccggccg cggtaccgcc cagggcgcca
     5461 aatgcagcgc cgaaattcgt tgcggtggca ctgccttcag acgccgaaat ctggactgcg
     5521 gagcgaatat catagagggt cagggtgaca accgaagctt ttgactgcag ggtgccagcc
     5581 accgcgccaa agatgccacc ggccatgctg cctaccgcag cgcccaggcc gccgaccgaa
     5641 tcattatcga taatgatctg cggttccatg tagtagtcag cggcaacgcg ttggcccttt
     5701 tgctgcttag accccgcgcg gtactcgcca gattcccgct gcatattggt gatgcgggac
     5761 aggcggctat ccgtcctgag gttgccaatg gacgtaataa cgaagcagtt tgattgctgg
     5821 accgcgaggc gaagcaacgg ctcgattgat gtaacgtgag tagtgctgcc aaattggcca
     5881 taccaatccg cattgcggcc gtcgtccatg gcaagcgtgc ctagcgggga atcgcaatgc
     5941 tcgagcgtat tggatgcccc cacgctcgat tcgccgccag ccgatccgct aactgcagtg
     6001 ccattagtgc ctgtggtgac gcacccgctc aatgtcgcgg ttgcaaacaa catgagggaa
     6061 atgcgtcgga tgctgaacgg ggtcatggtt tttaatggct cctttgcctt attagagtgg
     6121 ggtgaaaacg gggtctcgcc ggcgcacgcc ttcgacctcg gtggccagcg ttagtaaatc
     6181 caatacgttc cctgccagcg cccgaagtag gggtagccgc tataccaata gttatagaaa
     6241 atgccccgcc agtgctggcg ccatttccat tcggcttcgt ctgatctgcg ggcggcttct
     6301 gcttgtgcca ataatttcga cgcttcggca tcgccgcggc tggaagcaat ggagagccat
     6361 ttctgcgctt cccgagagtc tggccccatc tcttccaggc ctgtcaggta gacgcgcccc
     6421 agcgcctttt gtgcttccaa ttccccttgt tcggctgcgg cacgcatcca cgtgatggct
     6481 ttgtaactgt cctggggaac tccgtcgccc ctgaaatatc tcaatcccag atcgtaggcg
     6541 gcgcgggggg tttgctttgc aatgttctcc aacgcggcaa tgcggccatc atcgggaata
     6601 tcgttgagtt cctgggaaac ggcctggcga gatcggtcgc tgcacccttg agaattgcat
     6661 acccttaccg tgtcagcctg attggattgc gcgcaggacg ccaacaaaaa gcaaataatc
     6721 agtgcggcag gatgaatttt tttcacggca ggaaataaga aaagcagaga aataggacta
     6781 gagaaagcgt ttcaaagagc caatacgtac gtggcatgat tgggctgaag aataccgcca
     6841 agtaatcctt aaaaaaatct gagttatttt tttttataaa tatttgtatc gataatttaa
     6901 tggcctgggt taggccccga ctgattcgac aactattaaa gagatctgtt gcggatggcg
     6961 actccagata ggggataagc ttcttgcatg gaccccgggg aacatgcgtc gcctatatgg
     7021 gtcccttcta ctcactgcca cgggcttgac gagccatgac gcggccccgt agcgacgaac
     7081 ggcaggccat ttccgttgct tttctacatt cacccggcta ctttttgtaa ctccaaaaaa
     7141 gcacggaaat cgcagctacg accgcgtggc cttcattaga tgggcttacc gcatgcgagc
     7201 aaacgccatg agcgcaggca cagcaccgct atcgcctggg caagaaagcg cggcattgtc
     7261 ggcgacgaga agccaactcg caccaaacgg cgaggcaacc agtatttcga tactaggaga
     7321 attagtattt tcttcgctta ttgcgccatg ccgttctttc tatagtgcag ccatccggat
     7381 cgctccgcat gcgtggttaa tcacgcatcg tctgcgtcaa aaacgcgtct tgacgggata
     7441 ccaactgcca tgaacaacaa atcattcgtc ctaatcatcg cattgctggc gagcctggtg
     7501 gccggctgca ataccatcca cggcgccggc aaggacattg agcgcggcgg ggaaaaaatc
     7561 caggaaaaat cgcaatagcc acgtggataa cccgcgcgac cccccctgtg caggcggacc
     7621 gccgcgcggg ctgatccctg caaaaaacgc tcaagcatcg tgcaaccaca atgattcaaa
     7681 ccattgccgg acatttccgc agaataattt tgtttaacgc tgaaacttgc gaaaaatccg
     7741 gtcacgggta ttttaaataa agaagtaccg catcggcgtc atgagttttc tcggagttgc
     7801 ccaatacggc gccgacgacc ctcaacaagg agacaacatg cgatccatac ttttgtggct
     7861 attgggcgtt cctattccga tcatcatttt gatcgccctg ttctggcatt gattttcaat
     7921 gcgaatgact caacgagaaa aaggaaacgt catgcagaat gctcagccca ccacttcata
     7981 tactcacaca tcgaacggtg cggaatcgtc tcgttcggcc gtctcctgga gcgcagtcat
     8041 cgccggggcg gttatcgccg cagccgtatc ggcgatgttg atgacaggag gctccggact
     8101 cggatttctt tccatgtccc cttggcgcga tgaaggcgct tcgggttcgg cgcttgccat
     8161 tggatccatt gtttggttat tcgtgaccca gatcatcgcc tatggcgtcg cagggtatgt
     8221 cgcaggccga ttgcgcacga agtggacgga cacccacgat gacgaggtct acttccgcga
     8281 taccgcccat ggattcctgg tctgggcgct cagtgcggtg gtcggcttgg tgctattggg
     8341 gtcgacgatc gcgtcggtcg tttccggaac cgcgaaagcc ggagcgacgc tggcgggcgc
     8401 cggtgcgggc gcggcgaccg cggttgccgg acaagcggcg caagcggggg ccgaccatct
     8461 ctcgctggat tatttcaccg atgcattgct gcgcccgaac gatccggcgg cctccaacgg
     8521 cccgggcgac gtgcgccagg aggcatcgcg aattttggcc cgcagcctcg ccaacggcaa
     8581 cgtttccgat gaagaccagt cctatctggt caaggtgatc gcccagcgtg cgggcgtcga
     8641 cgaagccgcc gctaagcagc gcctcacgca agtcgaggaa caggccaagc aggccgcgca
     8701 gcaagccggg cagaaagcgc gagaagccgc cgacgcggca cgcaaggcgg cggcggcttt
     8761 cacgctctgg gccttcgcct ccttgctcgt cggcgctttc gtggcgagct tctgcgccac
     8821 gataggcggg cgcgcccgcg accgctgacg gccacaagac caggcgtctc gggtcaccgg
     8881 tatcgactgg cgtcccgaga cagcgaatat cggtcttatt ctccagtccg cgttgggggc
     8941 tccacatgaa ggacccgtcg tcgctatctt cgctgtcgga tcccgcggcg ctccgcgtat
     9001 ttctcggccg gcagggtact ggggccgata tctaccaggt gcttcagtcg ctggtcgagg
     9061 caggctatgg cgatctgcca cgcccgggat ccagccgtac gctcaggcgc tggcagatgc
     9121 tggccgccgc ggcgcaattc gacctgtccc tggccaagct ctacgagagc cacgccgacg
     9181 cgctggccat actccatgag ttggggcatg cggacctctc catgcgtggc ggaatctggg
     9241 gagtatggtg cgcggagccg cctagtcatc gcgtgcaggc ctgtcctcgt gattccggcg
     9301 gcaagtcggt gcgactgcac ggcgccaagg cttggtgctc cggcgccagc catgtcacgc
     9361 aggcactggt gagcgcatgg aacgaccaag ggcattcttg tctggttgca ctcgatctcg
     9421 accagcccga aatcgagatc gtccacgagg cgtggggcgc ggtcggcatg gccgcgaccg
     9481 ccagcgtgga catcgtgttt cacggcgccg caggcacgct ggtcggcgca ccgggcgcct
     9541 acctggaccg tccgggcttc taccacgggg ccgccggcgt cgccgcctgc tggtatggcg
     9601 cagccagcgc gattgcgcaa cgggtgctgg atagcgtccg aagccatcag gatgccccgc
     9661 actccctggc ccacctcggc gccctcgacc tcgtcctcac gcaagcccgt catcaattgc
     9721 gcattgccgc cgccgagatc gacgccaacc ctggggaaag ctgccagcag gccgtgcgtc
     9781 gcgcacgcct ggctgccgag tcggcggcgg aaacgatcct gatccgcgca ccgcgcgcct
     9841 tcggcccagg cccgatgtgc caagacgtcc gcatcgcgcg attgctcgcc gatcttcctg
     9901 tgttcatccg ccagagtcac gcggaacgcg acctgcaaac ccatggccag gcgctggccg
     9961 agcaggcgac ggaggcgtta tggacgctgt gagcacgcgc gcgattgccg gcgcaggcac
    10021 cccggaagcg tgctggcgcg cctggctgtc gcattatccg cctgcccggg gcgccgtcga
    10081 agacctggtc ctggcgcgtc atcgtctaca catcctcgca ccgcatcctg acgacgaggt
    10141 attgggatgc gccggaatca tgcgccaggc gctgcgcctg ggccgcccgg tacatgtctg
    10201 ggccatcacc gatggcgaag ccagtcatgc gggctcgcgc gcgatacgcg ccagcgagct
    10261 ggcgcaagtg cgcacccgag aaagcgagcg cgccttgcgc cggctcggaa caggtatccg
    10321 gcggcatcgc ctgaatttcc ccgatggcgg cgtcggccgg cacataccgg aaatcgcggg
    10381 catgcttgcc ccccatgtcc agcctggcga cacgatcatc tcgccctgga gcctggatgg
    10441 ccatcccgac catgaagcgg ccgcttgcgc gggcaggcaa gcagcccggg cacacggatg
    10501 ccggctattc gaagtcgtca tttggggctg gcactgggcc gatccgcaac gcggcgaatt
    10561 ccccatccag cgcgccgtgg ccattgcctt gacggcggcg gattgcctgg ccaaggcgcg
    10621 cgccatccag gcattccgca gccaattgga accggacccg gcaaccggca atccgcccat
    10681 actgccggat ttcgttctcg cccgtttcca gcgtcccttc gaggtggtgt tgcgatgacg
    10741 gacctcgact cccacttcga cgccctctac caggccagcg ccgacccctg gtcggtcacc
    10801 acggcgtggt acgagcgccg gaagcgggca acgctcatgg ccgcgcttcc tcgggaacga
    10861 taccggcacg cactcgagct gggttgcggc aacggcgcga ctacccgcct gctggctgag
    10921 agatgcgatg cggtccgtgc cgtggacagt tcgcccgccg cagtggcccg atgcgctcac
    10981 gcggtcgcgg cgggcggctt cacgaacgtc cagctggacg tctgcaggct gcccgatacc
    11041 tggccgttgc agcaaggcga accggtggat ctgatcgtcg tctcggaatt ggcgtactac
    11101 ttcaccgacg ccgatctgga tcgcctgctt tcgcgatgta ggcaggcgct ggcccaggac
    11161 ggcgattggc tcatgtgcca ttacacgcgc ccgtttcacg accggctcca ggataccgcg
    11221 cgaatccacg acaagataaa caacctgg
//
LOCUS       DASWPO010000159         6984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_571876, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000159 DASWPO010000000
VERSION     DASWPO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571876"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGC69_08765"
     CDS             complement(<1..383)
                     /locus_tag="VGC69_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295405.1"
                     /translation="MSALVLKIAIDRTPLTQALFDGAVASERVAFDFVEVKPITRAFR
                     RMARALEFDACEMALVTLAVAHRFAIPLTGIPAVLLREYPLSKLLCLRDSPVRQAADL
                     RGRRIAARSYSQTTVVWIRGLLETE"
     gene            complement(380..1114)
                     /locus_tag="VGC69_08770"
     CDS             complement(380..1114)
                     /locus_tag="VGC69_08770"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0295406.1"
                     /translation="MVDVLNRSVDRALTILKCYKAEDRGLTLAEVSRRTGIHKTTALR
                     LLATLESHRMIARLPSGMFALGAEVLRLGTLFGDTLEIAPIVRPFLQRLVQETGESAS
                     LFVKEGDFRRCLMREETTRTIREHVRVGDLLPLPLGAFGRVVDAFAAPPLDGERREPA
                     AVPIVTLAERVPGLGSIAMPVWQSGNRFCGAVGISIPLFRATDEVVEAAVRHVAAAGE
                     DLTRRLGGMAPEWAMPQHAHERRMNQ"
     gene            complement(1141..1818)
                     /locus_tag="VGC69_08775"
     CDS             complement(1141..1818)
                     /locus_tag="VGC69_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY0295407.1"
                     /translation="MAIGFRILERRQAVGAELAKRFAEVPVACISDVMSRLSAAGPTL
                     RPLHAGGVLCGPAFTVKTRPGDNLMVHKALMLAQPGDVIVVDAGGDLTNALVGELMLS
                     YGMQRGIAGVVINGAVRDSEWIGKHGFPVYAAGITHRGPYKDGPGEINVPVSLDGMVV
                     TPGDLMVGDADGLVCVPHDHAEDVYALAAKKRDGEAKVLANTLAGKLDAKAWVDETLR
                     KLGCEGL"
     gene            complement(1887..2921)
                     /locus_tag="VGC69_08780"
     CDS             complement(1887..2921)
                     /locus_tag="VGC69_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0295408.1"
                     /translation="MIIDIHGHYTTEPQALHHFRDKQLAGLLDPMRRPVSSDLGISDD
                     ALRESVAPQLKLQRERGSDLTIFSPRAAGMAHHVGTEETGKQWTTMSNDLIHRVCTLL
                     PDNFAPVGQLPQHPGVSPKHCIPELERIVQELGFVGVNLNPDPSGGYWTDPPLTDRHW
                     YPLYEKLVDLDVPAMIHVSSSCNPAFHHTGAHYINGDTTAFMQLLQGNLFKDFPTLRF
                     VIPHGGGAVPFHWGRYRGLAQELKKPPLPEHLLKNVFFDTCVYHLPGMELMAKVIPVE
                     NILFASEMLGAVKGVDPETGHNYDDTKRYVDALPKLGDAERYKIFEGNARRVYPRLDR
                     RLTERGRPGR"
     gene            complement(2992..3867)
                     /locus_tag="VGC69_08785"
     CDS             complement(2992..3867)
                     /locus_tag="VGC69_08785"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295409.1"
                     /translation="MTVPHKALHLRAALGDHAHVAPLKDGRVTSPLLEWEFIEFKPLP
                     AAFRRLLREGDLDLSEMAVASHLLGLDAGRPITALPIPLWGRLPHGNLLCLRDSPIRT
                     PRDLEGRRVGVRSYAQTSGVWVRGILQTAYGVDPDRITWVTLEDAHVAEFADPPNAIR
                     AAPGARLRDLLQAGEVQAIMGEREIDPAGVRTVIPDAEAAGRAWAASQRIDPIHHIVT
                     LRRELLAGRPWLAAELMHLFEQARRVAQGDGPAATPDYGLAPNRRSLDAILDYSYAQQ
                     LTRRRYAADDIFVAA"
     gene            complement(4016..4549)
                     /locus_tag="VGC69_08790"
     CDS             complement(4016..4549)
                     /locus_tag="VGC69_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEY0295410.1"
                     /translation="MALRATIYKAELHIADGDRHYYGSHSLTIARHPSETDERMMVRL
                     LAFALNAHEDLAFTKGLSDTDEPDVWRKALTGDIVDWIEVGQPEERRILKACGRAENV
                     LVYCYAHASKLWWDGIKNKVDRARNLRVIHFPSEQSQALAALAERTLQIHVNIQDGEA
                     YVSTDKGQVTVQPQAWR"
     gene            4685..5347
                     /locus_tag="VGC69_08795"
     CDS             4685..5347
                     /locus_tag="VGC69_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY0295411.1"
                     /translation="MTPATALLAFSIAAALLTLTPGLDTALVLRTAAGAGGRQALMAG
                     LGICLGCLAWGAVVALGLGSLLAVSSLAYDVLRYAGAAYLFWLGAKLLWHARARAASD
                     AAASPDADDASQAGPPSADSPWRWFARGVLTNVLNPKIGVFYATFLPQFIPAGVNVLG
                     FSLLLAGLHAAMGMAWFALLVAATRPLGRWLARPAVKRAMDRLTGLVFVAFGLRLALE
                     RR"
     gene            complement(5361..>6984)
                     /locus_tag="VGC69_08800"
     CDS             complement(5361..>6984)
                     /locus_tag="VGC69_08800"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY0295412.1"
                     /translation="FDGHDLLHLTEAEMRARRGRAISMIFQEPMTALNPAMPIGEQIA
                     EAVRVHHPKLSRADVRGRVLESLEQVALPDPARRIGDYPHQFSGGQRQRILIAMAVVN
                     KPQLLIADEPTTALDVTIQAQILQLIRQLQEEMDMGVIFITHDMGVVAEVADRVLVMY
                     RGKKVEEGTSDAVFAAPQHGYTRALLSAVPRLGAMHGTTQPAPFPLLRVDGSQAPAAP
                     GAGDAGTVRREQGPILRVNDLVTRFDIASGVLGRVEKRVHAVEKVSFDLYPGETLSLV
                     GESGCGKTTTGRSLLQLVKSQGGTIEFDGQDIGALRGPAMQAVRRHIQFIFQDPFASL
                     NPRMAVGDSIMEPLIIHRVARGREARERVRWLMEKCGLVPDMINRYPHEFSGGQRQRI
                     SIARALALNPKVVVADESVSALDVSIQAQIVNLLLDLQRELGVSFLFISHDMAVVERV
                     SHRVAVMYLGQIVEIGPRQAIFENPQHPYTRKLMAAVPIADPARRHRQRSLAVDEIPS
                     PIRAVGDEPVVRPLVQVGEGHFVARHPIGVYA"
BASE COUNT         1069 a         2353 c         2415 g         1147 t
ORIGIN      
        1 tattcggttt ccagcaggcc gcgtatccac acgacggtcg tctggctgta ggatctggcg
       61 gcgatgcggc ggccgcgcag gtcggctgcc tgccgcaccg gcgaatcgcg caggcagagc
      121 agcttggaca gcggatactc cctgagcagc acggccggaa tgccggtcag gggaatcgcg
      181 aaccgatgcg ccaccgccag cgtgaccagc gccatttcgc aggcgtcgaa ctccagcgcg
      241 cgcgccatgc ggcgaaaggc gcgggtaatg ggcttgacct ccacgaaatc gaacgcgacc
      301 cgctccgacg cgacggcgcc gtcgaacagg gcttgcgtca acggggttcg atcgatggca
      361 atcttcaata ccaatgcgct cattgattca tcctcctctc atgcgcatgc tgcggcatgg
      421 cccactcggg cgccatgccg cccagccgcc gggtcagatc ctcgccggcc gccgcgacat
      481 ggcgcaccgc cgcttcgacg acctcgtcgg tggcgcgaaa cagcgggatg gaaatcccca
      541 ccgcgccgca gaaccggttg cccgactgcc acacgggcat ggcgatcgaa cccagccctg
      601 gaacgcgctc ggccagcgtg acgatgggca ctgccgcggg ctcgcgccgc tcgccatcca
      661 agggcggcgc ggcaaaggcg tcgacgacgc ggccgaaggc gcccagcggc aacggcagca
      721 ggtcgcccac gcgcacgtgc tcgcggatcg tgcgggtggt ttcctcgcgc atcaggcagc
      781 ggcgaaagtc gccttccttg acgaacagcg aagcgctctc gcccgtctcc tgcaccagcc
      841 gctgcaggaa cggcctgacg atgggcgcta tctccagcgt atcgccgaac agcgtgccca
      901 gccgcagcac ctccgccccc aaggcgaaca tgcccgacgg caggcgcgcg atcatgcggt
      961 gggactccag cgtggccagc agccgcagcg cggtcgtctt gtggatgccg gtgcgccggg
     1021 aaacttccgc cagcgtcagg ccgcggtctt cggccttgta gcacttcagg atcgtcaggg
     1081 cgcggtcgac cgaacggttc aggacgtcga ccatgcctca gcctcggtgc gccacgatgt
     1141 tcacaaccct tcgcagccga gcttgcgcag cgtctcgtcc acccacgcct tcgcgtccag
     1201 cttgccggcc agggtattgg ccagcacctt ggcttcgccg tcgcgcttct tcgcggccag
     1261 ggcatagacg tcctcggcgt ggtcgtgggg cacgcacacc aggccgtcgg catcgcccac
     1321 catcaggtcg cccggcgtca cgaccatgcc gtccagcgac acgggcacgt tgatctcgcc
     1381 ggggccgtcc ttgtacgggc cgcgatgggt gatgccggcg gcgtagaccg ggaagccgtg
     1441 cttgcctatc cattccgaat cgcgcaccgc gccattgatg accacgccgg cgatgccgcg
     1501 ctgcatgccg tacgacagca tcagctcgcc caccagggcg ttggtcaggt cgccgccggc
     1561 gtcgaccacg atgacgtcgc ccggctgggc cagcatcagc gccttgtgca ccatcaggtt
     1621 gtcgcccggg cgggtcttga cggtgaaggc cgggccgcag agcacgccgc cggcatgcaa
     1681 cgggcgcagc gtcgggccag cggcgctcag ccgcgacatc acatcgctga tgcaggccac
     1741 gggaacctcg gcgaagcgct ttgccagttc cgcgccgacc gcctgcctgc gctcaagaat
     1801 tctgaatccg attgccatgt cttgggtacc ttgaaatggg tttgatcgat aggtgtccgt
     1861 ggactgccct ggcctgccgg cccggcttat cttcccggcc ggccgcgctc ggtcaggcgc
     1921 cggtccagcc tgggatagac ccggcgggca ttgccctcga agatcttgta gcgctccgcg
     1981 tcgccgagct ttggcagcgc gtcgacgtag cgcttggtgt cgtcgtagtt gtggcccgtt
     2041 tccgggtcca cgcccttcac cgcgcccagc atttccgagg cgaacaggat gttctccacc
     2101 gggatcacct tggccatcag ctccatgccg ggcaggtggt acacgcaggt gtcgaagaac
     2161 acgttcttga gcaggtgctc gggcagcggc ggcttcttga gttcctgggc caggccgcgg
     2221 tagcgccccc agtggaacgg cacggcgccg ccgccgtgcg ggatgacaaa gcgcagcgtg
     2281 gggaaatcct tgaacaggtt gccctgcagc agctgcatga acgccgtcgt gtcgccgttg
     2341 atgtagtgcg cgccggtatg gtggaaggcc ggattgcacg acgacgagac gtggatcatc
     2401 gcgggcacgt caaggtcgac cagcttttcg tacagcggat accagtggcg gtcggtcagg
     2461 ggcggatccg tccagtagcc gccggacgga tcggggttca ggttcacgcc gacgaagccc
     2521 agctcctgca cgatgcgttc cagctccggg atgcagtgct tcggcgacac gccggggtgc
     2581 tggggcaact ggccgacagg cgcgaaattg tccggcagca gcgtgcagac ccggtggatc
     2641 aggtcgttgg acatcgtggt ccactgcttg cccgtctcct ccgtgccgac atggtgcgcc
     2701 atgccggcgg cgcgcggcga aaagatggtc aggtcgctgc cgcgctcgcg ctgcagtttc
     2761 agctgcggcg ccacggattc ccgcagcgcg tcgtcggaaa tgcccaggtc ggacgaaacg
     2821 ggacggcgca tggggtccag cagcccggcg agctgcttgt cgcggaaatg gtgcagggcc
     2881 tgcggctcgg tcgtgtagtg gccgtggatg tcgatgatca gggggggatt gtgcgtgtcg
     2941 gtcataaatg gattccgggg caatggcggc cgatggcgtc aaaccagggt gtcaagcggc
     3001 cacgaagatg tcatcggccg catagcggcg gcgggtcaat tgctgggcgt agctgtagtc
     3061 caggatggca tccagcgagc ggcggttcgg cgccagcccg taatcgggcg tggcggccgg
     3121 cccgtcgccc tgcgccacgc gccgcgcctg ctcgaataaa tgcatcagct cggcggccag
     3181 ccacggccgg ccggccagca attcgcggcg cagcgtcacg atgtggtgga tgggatcgat
     3241 ccgctggctg gcggcccagg cccgtccggc ggcttcggca tccgggatga ccgtgcgcac
     3301 gccggcggga tcgatctcgc gctcgcccat gatggcctgc acctcgccgg cctgcagcag
     3361 gtcgcgcagc cgtgcacccg gcgcggcgcg gatggcgttg ggcggatcgg cgaattccgc
     3421 gacgtgcgca tcttccagcg tgacccaggt gatgcggtcc ggatccacgc cgtaggcggt
     3481 ctgcagaatg ccgcggaccc acacgcccga ggtctgcgcg taggaacgca cgcccacgcg
     3541 ccggccttcc aggtcgcgtg gcgtccggat gggagaatcc cgcaggcaaa gcagattgcc
     3601 atggggcagc cgtccccaca ggggaatggg cagcgcggtg atcgggcggc cggcgtcaag
     3661 ccccagcaga tggctggcga ccgccatttc cgacaggtcg aggtcgccct cgcgcagcag
     3721 gcggcggaaa gccgcgggca atggcttgaa ctcgatgaac tcccattcaa gcaggggcga
     3781 cgtgacgcgg ccatccttca gcggcgcgac gtgcgcgtgg tctccaagcg ccgcccgcag
     3841 gtgtagcgcc ttatgtggaa ctgtcatgga aaagctggcg gccttctctt tccggatgag
     3901 ccctttgatg cccgctcatg cactgaaaat gaaattgcgt ttcatacagt gatactaaaa
     3961 tgcgcaaagc ctggccgtca agccagcact ttcatttttt tgaatgcccc gcccgctagc
     4021 gccaggcttg cggctgcacc gtcacctggc ccttgtcggt ggagacgtag gcttcgccgt
     4081 cctggatgtt gacgtggatc tgcaaggtgc gctcggccag ggcggccagg gcctgcgact
     4141 gttcggacgg gaagtggatc acgcgcaggt tgcgggcgcg gtcgaccttg ttcttgatgc
     4201 cgtcccacca gagcttgctg gcgtgggcat agcagtagac gagcacgttt tccgcgcggc
     4261 cgcaggcctt gaggatgcgc cgctcctcgg gctggccgac ctcgatccag tcgacgatgt
     4321 cgccggtgag cgccttgcgc cagacgtcgg gctcgtcggt gtcgctcagg cccttggtga
     4381 aggccaggtc ttcatgggcg ttcaaggcga aggccaggag gcgcaccatc atgcgctcgt
     4441 cggtctcgga aggatggcgg gcgatggtca gggaatggct gccgtagtag tgcctatcgc
     4501 catcggcaat gtgcagttcg gctttgtaga tggttgcgcg cagggccatg ggacggattt
     4561 gatagaggcc ggcgccggcg gaaacggccg gcggcaagcc cgccatcata cctcgcgggc
     4621 atggcggcgg cggccactgc caccccggat aaactgactg ccgcgcccgc atagctccct
     4681 ttccatgacg cccgccaccg ccctgctcgc cttttcgatc gccgccgcgc tcctcacgct
     4741 tacccccggc ctggacaccg ccctggtgct gcgcaccgcc gccggcgccg gcgggcggca
     4801 agccctgatg gccggactgg gcatctgcct gggctgcctg gcatggggcg cggtggtggc
     4861 gctgggcctg gggtcgctgc tggccgtctc cagcctggcc tacgacgtgc tgcgctatgc
     4921 cggcgcggcg tatctgttct ggctgggtgc caagctgcta tggcatgcgc gggcgcgggc
     4981 cgccagcgat gccgccgcct cgcccgatgc cgacgatgca tcgcaggccg gcccgccctc
     5041 tgccgactcg ccgtggcgct ggttcgcgcg cggcgtcctg accaatgtgc tcaaccccaa
     5101 gatcggcgtg ttctacgcga ccttcttgcc gcagttcatc cccgccggcg tgaacgtgct
     5161 gggcttcagc ctgctgctgg ccggcctgca cgcggccatg ggcatggcct ggttcgcgct
     5221 gctggtggct gccacccgcc cgctggggcg ctggctggcg cgcccggcgg tcaagcgcgc
     5281 gatggaccgg ctgaccggcc tggtgttcgt ggccttcggg ctgcggctgg cgctggaacg
     5341 ccgttgaagg cagccgcccc tcaggcgtag acgccgatgg ggtggcgggc cacgaaatgg
     5401 ccctcgccca cctgcaccag gggccgcacg accggctcgt cgcccacggc ccggatgggg
     5461 ctggggatct cgtcgaccgc cagcgagcgc tggcgatggc ggcgcgccgg atcggcgatg
     5521 ggcacggcgg ccatcagctt cctggtatag gggtgctgcg ggttctcgaa gatggcctgg
     5581 cgcgggccga tctcgacgat ctggcccagg tacatcaccg ccacgcggtg gctgacgcgc
     5641 tcgaccaccg ccatgtcgtg cgagatgaag aggaaggaca cgcccagctc gcgctgcagg
     5701 tccagcagca ggttgacgat ctgcgcctgg atcgacacgt ccagcgccga cacggactcg
     5761 tcggccacca ccaccttggg gttgagcgcc agggcccgcg cgatgctgat gcgctggcgc
     5821 tggccgcccg agaactcgtg cggatagcgg ttgatcatgt cgggcacgag gccgcacttc
     5881 tccatcagcc agcgcacccg ttcgcgcgcc tcgcgaccgc gggccacgcg gtggatgatg
     5941 agcggctcca tgatggaatc gcccaccgcc atgcgcggat tcaaggaggc gaagggatcc
     6001 tggaaaatga actggatgtg ccggcgcacg gcctgcatgg ccggcccgcg cagggcgccg
     6061 atgtcctggc cgtcgaattc gatggtgccg ccctggctct tgaccagctg cagcagcgaa
     6121 cggcccgtgg tggtcttgcc gcagcccgac tcgcccacca gcgacagcgt ctcgccgggg
     6181 tagaggtcga aactgacctt ctccacggca tgcacgcgct tttcgacgcg gcccagcacg
     6241 ccgctggcga tgtcgaagcg cgtgaccaga tcgttcacgc gcaggatggg gccctgttcg
     6301 cggcgcaccg tgccggcgtc gccggcgccc ggcgccgcgg gcgcctggct gccgtccacg
     6361 cgcaagagcg gaaacggcgc cggctgcgtg gtgccgtgca tggcgcccag gcgcggcacg
     6421 gccgacagca gcgcgcgggt atagccgtgc tgcggcgcgg cgaaaaccgc gtcggacgtg
     6481 ccctcttcca ccttcttgcc gcgatacatc accagcacgc ggtcggccac ctcggccacc
     6541 acgcccatgt cgtgcgtgat gaagatcacg cccatgtcca tttcctcctg caactggcgg
     6601 atgagctgca ggatctgcgc ctggatggtc acgtccagcg cggtggtggg ctcgtcggcg
     6661 atcagcagtt gtggcttgtt gaccacagcc atcgcaatca ggatgcgctg acgctgtccg
     6721 ccggaaaact ggtgcggata gtcaccgatg cggcgggcgg ggtcgggcag cgcaacctgc
     6781 tccagcgact ccagaacgcg cccccgcacg tcggcgcgcg acagcttcgg gtggtggaca
     6841 cgcacagcct cggcaatctg ctcgccgata ggcatggccg gatttagcgc ggtcatgggc
     6901 tcctgaaaga tcatgctgat ggccctgccg cgacgcgcac gcatctccgc ctccgtcagg
     6961 tgcaggagat catggccgtc gaag
//
LOCUS       DASWPO010000160        10157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_659971, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000160 DASWPO010000000
VERSION     DASWPO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659971"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VGC69_08805"
     CDS             complement(<1..591)
                     /locus_tag="VGC69_08805"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486080.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HEY0295413.1"
                     /translation="MLEKYRQHVAERAALGIPPLPLSAQQTADLIELLKNPPAGEAEY
                     LLDLLTHRVPAGVDDAAKVKASYLAAVALGKEKNALISRAKATELLGTMLGGYNVAPL
                     IELLDDKEVGSVAADGLKKTLLMFDAFHDVQEKAEKGNANAKSVLQSWADAEWFTSRP
                     EVPQSLTVTVFKVPGETNTDDLSPAPDATTRPDIPLH"
     gene            885..1997
                     /gene="dusA"
                     /locus_tag="VGC69_08810"
     CDS             885..1997
                     /gene="dusA"
                     /locus_tag="VGC69_08810"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY0295414.1"
                     /translation="MSATAASALDAWRLSVAPMIDVTDRHCRYFHRLLAPRARLYTEM
                     ITTGALLHGDVARHLDFDPAEHPVALQLGGSEPDALARSARLGRQWGYDEINLNCGCP
                     SERVQKGAFGACLMAEPDLVADCVKAMQDAVDLPVTVKHRLGLDYDESYGFVRDFVGK
                     LYDVGCRVFVVHARNAVLKGLSPKDNREIPPLRYGTATQLKRDFPDCVVVLNGGLADA
                     DEAQQWLPGEPPGRSKASEALSPWGDADTQCERGAVFDGVMLGRAAWHTPRVLSELSL
                     RFWPAVRLPSDAQVVDAMTSYATRQVAAGVPLRVIVRPMLGLVNGQSGARRWRRLLSD
                     PAHLAANDPGLIYEAWRSLQSRHYEPEPAMAVQADC"
     gene            complement(2001..2459)
                     /locus_tag="VGC69_08815"
     CDS             complement(2001..2459)
                     /locus_tag="VGC69_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0295415.1"
                     /translation="MLKVREILRVKGDALYTATPDTPVSEAIRTMSKHDIGSMVIMEN
                     GMLVGMLTFREIIRLLNNQHEVGDTTIRAVMDDAPVSVSPNTEADEVQRLMLEKHARY
                     IPVMDGPTLQGVISFYDMAQAIVAAQKFENSMLKAYIRDWPSDSAEPAQA"
     gene            2543..4039
                     /locus_tag="VGC69_08820"
     CDS             2543..4039
                     /locus_tag="VGC69_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY0295416.1"
                     /translation="MTALRLADLATENTFAALPADFYSRLSPRPPDEPRLLHANPAVA
                     ALIGLDPAAFATPEFLAVCSGRMPLPGGDTLAAVYSGHQFGVWAGQLGDGRAHLLGEI
                     TGPECATSGSTPGNWELQLKGAGRTPYSRMGDGRAVLRSSVREYLAGEAMHGLGIPTT
                     RALALVVSDEPVARETMETAAVVTRMAPSFVRFGSFEHWSSNDRPDLLRILADYVIDK
                     FYPECREATGESGPVLALLEAVTRRTARLMAGWQAVGFCHGVMNTDNMSILGLTLDYG
                     PYGFMDAFQAGHICNHTDTQGRYAWNRQPSVALWNLYRLAGALHALVPDADALRGALD
                     GYEAVFTEAFHARMAARLGIAGWQAEDEPLLDDLLRLMHENRADFTLSFRRLAGVVRG
                     EPRGFLDLFIDREAAAAWLARLQARHAQAGGEAGARAAGMDRVNPLYVLRNHLAELAI
                     RAAKEDDASEIDTLLRLLADPCTERPGYERYAELPPEWATGIEVSCSS"
     gene            complement(4073..5236)
                     /locus_tag="VGC69_08825"
     CDS             complement(4073..5236)
                     /locus_tag="VGC69_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295417.1"
                     /translation="MRILVTLMAIATLAPALALADPVKIGIAADISGSFAALGAEARD
                     GYNLALKQLGNKVGGQPVEIVQADTGGNPDQARQLVSRFIQRDKIDFFTGPIGSNVAL
                     AVGPALFAAKIPYLSNNPGPSQYSGAQCNAYWFSLSYQNDAPSEAAGKVAADRGFKKV
                     FSVAPDYPAGKDVVAGFKLGYALPLAAEVYTKLGQVDYSAELAEIRAARPDVVFLFLP
                     GGMGINFIKQYAGAGLSPAIPLLGSGFSGEEDVIRAVGEPMLGMYNTMSWAHDLDVPA
                     NQKFVAAFRQEYQGRYPSVYAANAYDVIMAIDAAVSQTGGKVSDRDAVVKALEKADFP
                     SVRGHFAYGRNHFPIQPYYVRQIVKDSQGVITNKLAGKALDSYQDKHLGECKL"
     gene            complement(5248..6783)
                     /locus_tag="VGC69_08830"
     CDS             complement(5248..6783)
                     /locus_tag="VGC69_08830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0295418.1"
                     /translation="MTLQASTSAIHAFVAQIRDELGESAISLAEETLTRYGEHTLPAA
                     DVAPIAVAYPASTQDVRKLVLAANRHKVPLFPISAGQNIGLGSRAAVLPGQVIVDLGR
                     NMQRILEVNETLGYCVIEPGVSFQALHDELKKRGDKLMMSPTAGPPQGSVIGNAMDKG
                     GGYGPAADHFGAICGMEIVLGNGEIIRTGDGSLDSPEQLNWHVSKYSFGPTLDGLFAQ
                     SNYGIVTRVGMWLMPRPPHIASFFFSFPDDDDLGEIIDLIRPLKLSNFVPVMMRATND
                     LYLSAAEEKHPEYAATQGRKALSDAARKTLQRQHGIGAWTVSGALYGASAQAVQPQID
                     RLRKHFTQSGKGRYIDPAQAEDMAPLDISRDNYAGAPGEGELGMLRWRPGGGAVWFTP
                     GLPMVGSVANEFRRLSEDICRDHGLEYMVSNVCGARFARGVHVILFNRTDPDESARAD
                     ACYRALSDMFSRRGVFVGRAPTAYQPFHQQRRMPAFNAACSAIKQALDPNDIIAPGKY
                     GIE"
     gene            complement(6789..8189)
                     /gene="glcF"
                     /locus_tag="VGC69_08835"
     CDS             complement(6789..8189)
                     /gene="glcF"
                     /locus_tag="VGC69_08835"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY0295419.1"
                     /translation="MTVSSGYIPISRLDQGMKVADACVHYGFCTAVCPTYVLDGEENE
                     SPRGRIGLIKTMLASGKRPDPATVTHIDRCLSCLSCASTCAAGVEYRTLIDTAREYVE
                     QSGVRPWPERLWRAGLSRMLTSPRLLRVMLRLGRPAGKATARLPGRFGALGRMAKAPG
                     LDAVAHRPAALAQIDQPTATRTVALLEGCVQSALGPEINQAARRIFARLGIGVADAPG
                     ALAGACCGALDLHMGQRERARRQAARWVMHWSELMAQGVIDRIVVTTSGCGSVIKHYP
                     ELFDDDDDLARRARQVAQACVDASEFLADADLPRQDAHLGARVAYHDSCSLKHGQNVT
                     RQPRAVLRRLGFKVLDVPESHLCCGSAGTYNLLQPAIADRLGARKAANIAGLGADVMA
                     AANLGCIVQISRFLGTPVAHWIQLVDWAGGGPAPLGLAGHVPLRPEPEEPEAGPPAAE
                     TSGAGADTAPDSNSFW"
     gene            8370..9341
                     /locus_tag="VGC69_08840"
     CDS             8370..9341
                     /locus_tag="VGC69_08840"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0295420.1"
                     /translation="MPSWRRWQADGPAQKRTSYWQDVVCETMLHVTMAPGEPADARPF
                     DGILESCAQADARLVNFRSSAHRITRDARQAGLGNDQYIIVGLQRRGMTSLSQRHGEV
                     RLAYGDIGVIDGTQPFQMQFADTIERCVVLLPRLVLKDRLPRFGGLKAPLRLGADDAV
                     TAILARTVQALTTEHSSFDDGSASVLLSHIADLLALRMTDSAGQPELPGRLAYENLLR
                     IIDRHLGMAGLTPAYAAAQARMSLRTAHRLFRQYSEPRISIEKCIFERRLVRAYQALA
                     QGKARNVSDAAFTAGFADLSHFTRRFGERFGISPSALLPGAGAGPDL"
     gene            9416..>10157
                     /gene="aroC"
                     /locus_tag="VGC69_08845"
     CDS             9416..>10157
                     /gene="aroC"
                     /locus_tag="VGC69_08845"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0295421.1"
                     /translation="MSGNTLGTLFRVTNFGESHGPAIGCVVDGCPPGLPLDAADIQAE
                     LDRRRPGTSRHVTQRQEADQVEILSGLYEGVTTGTPIGLLIRNTDQRSKDYGNLLDTF
                     RPGHADYAYWKKYGVRDPRGGGRASARLTAPTVAAGAIAKQWLARECGVKVRGYMSQL
                     GPIGIPFETWDAVGENAFFAPNAGIVPELEAYMDQLRRDGDSVGARIEVVAESVPAGW
                     GEPIYDRLDADIAHAMMGLNAVKGVSIGD"
BASE COUNT         1535 a         3409 c         3450 g         1763 t
ORIGIN      
        1 gtgcagggga atgtcggggc gcgtggtggc gtcgggcgcg ggagaaagat cgtcggtgtt
       61 ggtttcgccg ggcaccttga acacggtcac ggtcaggctt tgcggcactt ccggacggct
      121 ggtgaaccac tcggcgtcgg cccagctttg cagcacggac ttggcgttgg cgttgccctt
      181 ttcggccttt tcctgcacgt cgtggaaggc gtcgaacatc agcagggttt tcttcaggcc
      241 gtcggcggcc acggaaccca cttccttgtc gtcgagcagc tcgatcagcg gggcgacgtt
      301 gtagccgccc agcatggtgc ccagcagctc ggtggccttg gcgcggctga tcagggcgtt
      361 cttttccttg cccagggcca cggcggccag gtaggaggcc ttgaccttgg cggcatcgtc
      421 gacgccggcc ggcacgcggt gcgtcagcag gtcgagcagg tactcggctt cgccggcggg
      481 cggattcttc agcagttcga tcagatctgc ggtctgttgg gccgacaggg gcaggggcgg
      541 gatgcccagg gctgcgcgtt cggcgacgtg ttgacggtac ttttccagca tgggaggcct
      601 gcgtaaaaag tgggttggtg gaaattttcc gccattgtag aaggaagccg gattgggagc
      661 aacagtctta tatcttatat aagacttaaa ggattgcgtc ccggattgcg ttccctcaac
      721 cccggattgc cgctcctggc ccgctcgtcg cgcggccctt tgcacggcga accgtcaccg
      781 taaaatcgcc ccgcgctcgc ggcgaaccgt tcccgcgacc ggcggctgga gaacccaaaa
      841 aacaatcccc ccagccagtt cccccacaat gcgataattc tggaatgtcc gccaccgcag
      901 cctctgctct tgacgcttgg cgcctttccg ttgcgcccat gatcgacgtc accgatcgcc
      961 attgccgcta tttccaccgg ctgctggcgc cgcgcgcgcg gctctatacc gagatgatca
     1021 ccacgggcgc gctgctgcac ggcgatgtcg cccggcacct ggatttcgac ccggccgagc
     1081 accccgtggc cctgcaactg ggcggcagcg agcccgacgc cctggcccgg tcggccaggc
     1141 tgggccggca gtggggctac gacgagatca acctcaactg cggctgcccg tccgagcggg
     1201 tgcagaaggg cgcctttggc gcctgcctga tggccgaacc cgatctggtg gccgactgcg
     1261 tcaaggccat gcaggacgcg gtcgacctcc ccgtcacggt caagcatcgg ctggggctgg
     1321 actacgacga gtcctacgga ttcgtgcgcg atttcgtcgg caagctctac gacgtgggct
     1381 gccgcgtctt cgtggtgcat gcgcgcaacg cggtgctcaa gggcctttcg cccaaggaca
     1441 accgcgagat cccgccgctg cgctacggca cggccacgca gctcaagcgc gactttcccg
     1501 attgcgtggt ggtgctcaac ggcggcctgg ccgatgcgga cgaggcacag caatggttgc
     1561 cgggcgagcc gccgggccgc tccaaggcga gcgaagcgct ttccccttgg ggggacgcgg
     1621 acacgcagtg tgagcggggg gctgtattcg atggcgtgat gctgggccgc gccgcctggc
     1681 ataccccgcg cgtgctgtcc gagctgtcgc tgcgcttctg gcccgcggtg cgcttgccgt
     1741 cggacgcgca ggtggtcgac gccatgacgt cctatgccac ccggcaagtg gccgcgggcg
     1801 tgcccctgcg ggtgatcgtg cggcccatgc tgggcctggt caacggccag tcgggcgcgc
     1861 gccgctggcg ccgcctgctg tcggatccgg cccatctggc ggccaacgat cccggcctga
     1921 tctacgaggc ctggcgcagc ctgcagagcc ggcactacga acccgaaccc gccatggccg
     1981 tgcaggcgga ctgctgatcc tcaggcctgc gccggttcgg cgctgtcgct cggccagtcg
     2041 cggatatagg ccttgagcat gctgttctcg aacttctgcg ccgccacgat ggcctgggcc
     2101 atgtcgtaga acgagatcac gccttgcagg gtggggccgt ccatgacggg aatgtagcgg
     2161 gcatgctttt ccagcatcag gcgctgcacc tcgtcggcct cggtattggg cgagacgctc
     2221 acgggcgcgt cgtccatgac ggcgcggatg gtggtgtcgc cgacctcgtg ctggttgttc
     2281 agcaggcgga tgatttcgcg gaaggtgagc atgcccacca gcatgccgtt ttccatgatc
     2341 accatggaac cgatgtcatg cttgctcatg gtgcggatgg cttccgacac cggcgtgtcg
     2401 ggcgttgcgg tgtacagggc gtcgcccttc acgcgcagga tttctctgac tttcagcatg
     2461 gcgttcctcc tcaggcttta ttgtgcgcca gccgggcggg ttcttccagc ccgaaggccg
     2521 cgcatgcggt gataatgatg ccatgactgc cttgcgcctg gccgacctgg ccactgaaaa
     2581 caccttcgcg gccctgccgg ccgatttcta ctcccggctg tcgccgcgcc cgccggacga
     2641 gccgcggctg ctgcacgcca atccggccgt ggccgcgctg atcgggctcg accctgccgc
     2701 cttcgccacg ccggaattcc tcgcggtgtg ctcgggccgc atgccgctgc ccggcggcga
     2761 cacgctggcg gccgtctaca gcggccacca gttcggcgtc tgggccggcc aactgggcga
     2821 cgggcgggcg cacctgctgg gggaaataac ggggcctgaa tgtgccacgt ccggttccac
     2881 ccccggcaac tgggagctgc agctcaaggg cgcgggccgc acgccttact cccggatggg
     2941 ggacggacgg gccgtgctgc gctcgtcggt gcgcgaatac ctggccggcg aggccatgca
     3001 cggcctgggc atacccacca cgcgggcgct ggcgctggtg gtgtccgacg agccggtcgc
     3061 gcgcgagacc atggaaaccg ccgccgtcgt cacgcgcatg gcgcccagct tcgtgcgctt
     3121 cggctcgttc gaacactggt cttccaacga ccggcccgac ctgttgcgca tcctggccga
     3181 ctacgtcatc gacaagttct accccgaatg ccgcgaggcg accggcgaat cgggcccggt
     3241 cctggccctg ctggaggccg tcacgcgccg cacggcgcgg ctcatggccg gctggcaggc
     3301 cgtaggtttt tgccacggcg tgatgaacac cgacaatatg tccattctgg ggctgacgct
     3361 ggactacggc ccctacggtt tcatggatgc cttccaggcc ggccacatct gcaatcacac
     3421 cgatacgcag ggtcgctacg cctggaaccg ccagccctcg gtggcgctgt ggaacctcta
     3481 tcgcctggcc ggcgccctgc acgcgctggt gccggatgcc gacgcgctgc gcggcgcgct
     3541 cgacggctac gaggccgtgt tcaccgaggc cttccacgcc cgcatggccg ccaggctggg
     3601 catcgccggc tggcaggccg aggacgagcc gctgctcgac gacctgctgc gtctcatgca
     3661 cgagaaccgc gccgatttca ccctgagctt tcgccgcctg gccggcgtgg tgcgcggcga
     3721 gccgcgcggc tttctcgacc tgttcatcga ccgcgaagcg gcggcggcct ggctggcccg
     3781 cctgcaggcc cgccacgcgc aagcgggcgg ggaggccggc gcgcgcgcgg ccggcatgga
     3841 ccgggtcaac cccctgtacg tgctgcgcaa ccacctggcc gaactggcga tccgcgccgc
     3901 caaggaagac gatgcttccg agatcgacac cctgctgcgc ttgctggccg acccctgcac
     3961 cgagcggccg ggctacgagc gctacgccga attgccgccg gaatgggcaa ccggcatcga
     4021 ggtcagttgc tcgtcctgag cccgtccgcg gcctgcggcg cgggccgtcc gctcaaagct
     4081 tgcattcgcc caggtgcttg tcctggtagc tgtcgagcgc cttgcccgcc agcttgttgg
     4141 tgatcacccc ctggctgtcc ttgacgatct ggcgcacgta ataaggctgg atggggaaat
     4201 ggttgcggcc gtaggcaaaa tgtccgcgca cggaaggaaa gtccgctttc tccagcgcct
     4261 tgaccacggc atcgcggtcg gaaaccttgc cgcccgtctg gctgaccgcg gcatcgatgg
     4321 ccatgatcac gtcgtaggca ttcgcggcat acaccgaagg gtagcggccc tggtattcct
     4381 ggcgaaaggc cgccacgaac ttctggttcg cgggcacgtc caggtcgtgc gcccatgaca
     4441 tggtgttgta catgcccagc atgggctcgc ccacggcgcg gatcacgtcc tcttcgcccg
     4501 agaagcccga gccgagcagg ggaatggcag gggacaggcc cgcgcccgcg tactgcttga
     4561 tgaagttgat gcccatgccg ccgggcagga ataggaagac gacgtcgggc ctggcggcgc
     4621 ggatctcggc cagttcggcc gagtagtcga cctggcccag cttggtgtag acctcggcgg
     4681 ccaagggcag cgcgtagccg agcttgaagc cggcgacgac atccttgccg gcgggataat
     4741 ccggcgcaac gctgaacacc ttcttgaagc cgcggtccgc ggcgaccttg ccggcggctt
     4801 cggacggcgc gtcgttctga taagacaaag agaaccagta cgcgttgcac tgcgcgccgg
     4861 aatactggct ggggccgggg ttgttcgaca ggtaggggat cttggccgcg aagagcgccg
     4921 gtcccacggc cagcgccacg ttcgaaccga tgggcccggt gaagaaatcg atcttgtcgc
     4981 gctggatgaa gcgcgagacc agttgacgag cctggtcggg attgccgccc gtgtcggctt
     5041 gcacgatctc gaccggctgc ccgcccacct tgttgcccag ctgcttgagc gccaggttgt
     5101 agccgtcgcg ggcttcggcg cccagcgccg cgaaagagcc cgagatatcg gcggcgatgc
     5161 cgatcttgac gggatcggcc aaggccaggg cgggcgccag ggtggcgata gccattagcg
     5221 taacgagaat gcgcattgct tgagctccta ttcgatgccg tatttgcccg gcgcgatgat
     5281 gtcgtttgga tccagggctt gcttgatggc tgagcacgcc gcgttgaacg ccggcatgcg
     5341 gcgctgctgg tggaatggtt gataggccgt gggcgcccgc cccacgaaca cgccgcggcg
     5401 cgagaacatg tccgacaggg cgcggtagca cgcgtcggcg cgcgcgcttt cgtcgggatc
     5461 ggtccggttg aagaggatca cgtgcacgcc gcgcgcgaac cgcgcgccgc agacgttcga
     5521 caccatgtat tccagcccat ggtcgcggca gatgtcttcc gacaggcggc ggaattcatt
     5581 ggcgacgctt cccaccatgg gcaggcccgg cgtgaaccag acggccccgc cgcccgggcg
     5641 ccagcgcagc attcccagtt cgccttcgcc cggcgcgccc gcgtagttgt cccgcgagat
     5701 atcgagcggc gccatgtcct cggcctgcgc cgggtcgatg tagcgcccct tgccggactg
     5761 ggtgaaatgc ttgcgcaacc tatcgatttg cggctgcacg gcctgcgcgc tggcgccata
     5821 cagggcgccg gagacggtcc aggcgccgat tccatgctgg cgctgcaggg ttttgcgggc
     5881 ggcgtcgctg agcgccttcc tgccctgcgt ggcggcatat tcggggtgct tttcctccgc
     5941 cgcgctcagg tagagatcat tggtcgcgcg catcatcacc ggaacgaagt tcgacagttt
     6001 cagcggccgg atcaggtcga tgatttctcc caggtcatcg tcgtccggga aggagaagaa
     6061 aaaggacgcg atgtgcggcg gcctgggcat cagccacatg cccacgcgcg tcacgatgcc
     6121 gtaattcgac tgggcgaaca gtccgtccag ggtcggcccg aaactgtatt tggagacatg
     6181 ccaattcagt tgctccggcg agtcgaggct gccgtcgccg gtgcggatga tctcgccgtt
     6241 gcccagcacg atctccatgc cgcatatcgc gccgaaatgg tccgccgccg ggccgtagcc
     6301 gccgcccttg tccatcgcat tgccgatcac gctgccttgc ggcgggccgg cggtgggcga
     6361 catcatcaac ttgtcgccgc gctttttcag ctcgtcatgc agggcctgga agctcacccc
     6421 gggttcgatc acgcaatagc cgagcgtctc gttgacctcg agtatgcgtt gcatgttgcg
     6481 gcccaggtcc acgatgacct ggccgggcag cacggcggcc ctggagccca ggccgatgtt
     6541 ctgcccggcg ctgatgggga acagcggcac cttgtgccgg ttggccgcca gcacgagctt
     6601 gcgcacatcc tgcgtcgagg cgggatacgc caccgcgatg ggcgcgacgt ccgcggcggg
     6661 cagcgtgtgt tcgccatagc gcgtcagcgt ttcttcggcg agcgagatcg cgctttctcc
     6721 cagttcgtcg cgtatttgcg cgacgaatgc gtgaatggcg ctcgtggagg cttgcagggt
     6781 catgagggtc accagaaact gttgctgtcg ggagcggtat ccgcgcctgc gccggacgtt
     6841 tccgcggcag gggggccggc ctcgggttct tcgggttccg gccgcagcgg aacatggcct
     6901 gccaggccga gcggcgcggg tccgccgccg gcccagtcga ccagctgtat ccaatgcgcc
     6961 accggcgtgc cgaggaaccg tgaaatctgc acgatgcagc ccaggttggc ggcagccatg
     7021 acgtcggcgc ccagtcccgc gatattggcc gccttgcggg cgcccaggcg atcggcgatg
     7081 gcgggctgca gcaggttgta ggtgccggcc gatccgcagc acaggtggct ctccggcaca
     7141 tcgagcacct tgaagccgag gcggcgcagc accgcgcgcg gttgccgggt gacgttctgg
     7201 ccatgcttga gcgagcagga atcgtgatag gccacgcgcg cgccgagatg ggcatcctgg
     7261 cggggcaggt cggcatccgc gagaaattcg gacgcatcga cgcaggcctg cgcgacttgc
     7321 cgcgcgcgcc gcgcgagatc gtcgtcatcg tcgaagagct cggggtagtg cttgatgacg
     7381 gagccgcagc ccgaggtcgt gaccacgatg cggtcgatga cgccttgcgc catcagttcc
     7441 gaccagtgca tgacccagcg cgcggcttgc cgccgtgccc gctcgcgctg tcccatgtgc
     7501 aggtcgagcg cgccgcagca ggcgccggcc aaggcgccgg gcgcgtcggc gacgcctatg
     7561 cccaggcggg cgaagatgcg gcgcgccgcc tggttgatct ccggtcccag ggcggattgc
     7621 acgcagcctt ccagcagcgc gacggtccgc gtggcggtgg gttgatcgat ctgcgccagg
     7681 gcggcgggcc tgtgcgccac ggcgtccagg cccggtgcct tggccatgcg tcccagcgcg
     7741 ccgaagcgtc ccggaagccg ggcggtcgct ttgccggcgg ggcgtcccag gcgcagcatg
     7801 acccgcagca gccgggggga ggtcagcatg cgcgacaagc ccgcgcgcca caggcgttcc
     7861 ggccaggggc gcacgcccga ttgctccacg tattcgcgcg cggtgtcgat cagcgtgcgg
     7921 tattcgacgc cggccgcgca ggtgctggcg cacgacaggc aggaaaggca gcgatcgata
     7981 tgcgtgacgg tggccggatc cggccgcttg ccgcttgcca gcatggtctt gatcaggccg
     8041 atgcggccgc gcggcgattc gttttcttcg ccgtccagca cataggtcgg acagaccgcg
     8101 gtgcaaaagc cgtagtgcac gcaggcatcg gctactttca ttccctgatc caggcgcgag
     8161 atcgggatgt agccgcttga cacagtcatg tcgggctctc catacttggt tcgtattttg
     8221 gaatcggata gcggggaagc cgcgttgcgc ttcggcctgg atttgagtgt gcccccgcat
     8281 gccgcgcctg gcttggccga tcgcgtcacc cgtatttgcc cagcgtgcca tccgatctag
     8341 cgaaaacccg cataccctct tcaagcgata tgccctcatg gcgacgttgg caggctgacg
     8401 gtcctgccca gaaacgcacc tcgtactggc aggatgtcgt ctgcgagacg atgctgcacg
     8461 tcaccatggc gcctggcgag ccggccgatg cgcggccttt cgacggaata ctcgagagct
     8521 gcgcgcaggc cgacgcgcgc ctggtgaatt tccgctcgtc ggcccaccgg atcacgcgcg
     8581 atgcgcgcca ggcaggcctg ggcaacgacc aatacatcat cgtcggcctg cagcgccggg
     8641 gcatgacgag cctgtcgcag cgccatggcg aggtgcgtct ggcttatggc gacatcgggg
     8701 tgatcgacgg cacgcagcct ttccagatgc aattcgcgga caccatcgag cgctgcgtcg
     8761 tcctgctgcc gcgccttgtc ctgaaagacc gcctgccgcg tttcggcggg ctcaaggcgc
     8821 ctttgcgcct gggcgccgat gacgccgtca ccgcgatcct ggcccgcacc gtgcaggcgc
     8881 tgaccacgga gcacagttcc ttcgacgacg gcagcgcgag cgtactgctg tcgcacatcg
     8941 ccgatctgct cgccttgcgc atgacggact cggccggcca gcccgagctg cccggccgcc
     9001 tggcttatga aaacctgctg cgcatcattg accgccatct gggcatggcc ggtcttactc
     9061 ccgcgtacgc cgcggcgcag gcgcgcatgt cgctgcgcac cgcgcaccgg cttttcaggc
     9121 agtacagcga gccgcgcatc agcatcgaga aatgcatctt cgaacggcgc ctggtgcggg
     9181 cctatcaggc cctggcgcag ggcaaggcgc gcaatgtgtc ggacgccgcg ttcacggccg
     9241 gctttgccga tctgtcgcat ttcacccggc gtttcggcga acgcttcggc atttcgccct
     9301 cggcgctgct gcccggggca ggtgccgggc ccgatttgta acgtctcgga taaaatcgcc
     9361 acaggcggcc tgccaggccg attttccgcg cctcgcgcgg tgcgatgatg attccatgtc
     9421 cggcaacact ctaggcactc ttttccgcgt caccaacttc ggcgaatccc acggcccggc
     9481 catcggctgc gtggtcgacg gctgcccccc gggcctgccg ctggacgcgg ccgacatcca
     9541 ggccgaactc gaccgccgcc gtcccggcac gtcgcgccac gtgacccagc gccaggaagc
     9601 ggaccaggtc gagatcctgt cgggcctgta cgagggcgtc accaccggca cgcccatcgg
     9661 cctgctgatc cgcaataccg accagcgcag caaggactac ggcaatctcc tcgacacctt
     9721 ccgtccgggg catgccgact acgcctactg gaagaaatat ggcgtgcgcg atccgcgcgg
     9781 cggtggccgc gcgtcggccc gtctcacggc gcccaccgtg gcggccggcg ccatcgccaa
     9841 gcaatggctg gcgcgcgaat gcggcgtcaa ggtgcgcggc tacatgagcc agctcggccc
     9901 catcggaatt cccttcgaaa cctgggacgc ggtgggggag aacgcctttt tcgcgcccaa
     9961 tgccggcatc gtccccgagc tcgaggccta tatggaccaa ctgcgccgcg acggcgattc
    10021 ggtcggcgcg cgcatcgagg tcgtggccga gtccgtgccc gccggctggg gcgagcccat
    10081 ctacgaccgg ctcgatgccg acatcgccca cgccatgatg ggcctgaacg ccgtcaaggg
    10141 cgtgtccatc ggcgatg
//
LOCUS       DASWPO010000161        11565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2893742, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000161 DASWPO010000000
VERSION     DASWPO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893742"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /gene="acnA"
                     /locus_tag="VGC69_08850"
     CDS             <1..1673
                     /gene="acnA"
                     /locus_tag="VGC69_08850"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY0295422.1"
                     /translation="PQTIAYLKLTGREDEQVKLVETYARTAGLWADSLKNVQYERVLS
                     FDLSSVVRTLAGPSNPHRRLPVSDLAARGISGIVENTPGQMPDGAVIIAAITSCTNTS
                     NPRNVIAAGLLARNANRAGLTRKPWVKSSLAPGSKTVALYLDEAGLTSELEKLGFGVV
                     AFACTTCNGMSGALDPKIQQEIIARDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLV
                     VAYAIAGTIRFDIERDVLGVDAAGKEIRLKDIWPSDEEIDAVVAASVKPEQYRKVYDV
                     MFAVHDQRGEAVSPLYAWRPQSTYIRRPPYWEGALAGERSLRGMRPLALLGDNITTDH
                     LSPSNAILPDSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRATFANPTLVNEMALAA
                     DGSVKKGSLARVEPEGKTMRMWEAIETYMQRKQPLIIVAGADYGQGSSRDWAAKGVRL
                     AGVEAIAAEGFERIHRTNLIGMGVLPLEFKPGVNRKTLGLDGTETYDVVGERKPRADL
                     TLVIHRKNGETLQVPVTCRLDTAEEVSIYEAGGVLQRFAQDFLEAARA"
     gene            1800..2990
                     /gene="prpF"
                     /locus_tag="VGC69_08855"
     CDS             1800..2990
                     /gene="prpF"
                     /locus_tag="VGC69_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEY0295423.1"
                     /translation="MTHAPQVRIPATYMRGGTSKGVFFKLTDLPEAARVPGAVRDALL
                     MRVIGSPDPYGKQIDGMGAATSSTSKTVIVGKSTRKDHDVDYLFGQVAIDRAFVDWTG
                     NCGNLTAAVGPFAISNGLIDSARVPKNGVAVIRIWQANIGKTIIAHVPMTDGMVQETG
                     DFELDGVTFPAAEVPLEFMAPADDGEDGGAMFPTGNLVDKLEVPDVGTFNATMINAGI
                     PTIFLNAQDLGYTGTELQGDINGDPQALKRLETIRAYGALRMGLIKSLDEIAGRQHTP
                     KVAFVAPPASYTASSGKKIDAKDIDVLARAMSMGMLHHAMMGTASVAIATAAAVPGTL
                     VNLAAGGGERNAVRFGHPSGVLRVGAEARLVDGQWVVNKALMSRSARVMMEGWVRVPL
                     ETIV"
     gene            3372..3659
                     /locus_tag="VGC69_08860"
     CDS             3372..3659
                     /locus_tag="VGC69_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0295424.1"
                     /translation="MKSKSSASTSRSTPPRLRVGLKDVKQEGFAAAVQEKLGKQRVSI
                     MLDTSVIAFFKAKAGDRGYQTLINQALHQAMLGEQLEATLRRVIREELHPA"
     gene            complement(3739..4935)
                     /gene="prpC"
                     /locus_tag="VGC69_08865"
     CDS             complement(3739..4935)
                     /gene="prpC"
                     /locus_tag="VGC69_08865"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEY0295425.1"
                     /translation="MSTAPKKKAAAATPAPEAAPSGFKPKKSVALSGVVAGNTALCTV
                     GRTGNDLHYRGYDILDFASDAEFEEIAHLLVHGKLPNKAELKAYKEKLRSLRGLPAPL
                     QDALEALPAASHPMDVMRTAVSVLGCVLPEKDDHNIPGARDIADRLMANLGSALLYWY
                     HYSHNGRVIDVQTDDDSIGGHFLHLLHGKKPSEDWVRAMHTSLILYAEHEFNASTFTC
                     RVVAGTGSDMYSAITGGIGALRGPKHGGANEVAFEVQSRYDTLDEAEADIRQRVENKE
                     VIIGFGHPVYTVSDPRNKVIKEVSRKLSKQAGSTKLFDIAERLETVMWDIKKMFPNLD
                     WFSAVSYHMMGVPTAMFTPLFVIARTAGWSAHIIEQRVDNKIIRPTANYIGPEDQKFV
                     SIEKRK"
     gene            complement(5019..5912)
                     /gene="prpB"
                     /locus_tag="VGC69_08870"
     CDS             complement(5019..5912)
                     /gene="prpB"
                     /locus_tag="VGC69_08870"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY0295426.1"
                     /translation="MTRPASPGALFRQALAEEQPLQIIGTINANHALLAKRAGYRAIY
                     LSGGGVAAGSLGLPDLGINTMDDVLIDVRRITDVCDLPLLVDIDTGFGPSAFNIARTI
                     KSLIKSGAAACHIEDQVGAKRCGHRPGKEIVSTEEMADRVKAAADARTDSDFYLVART
                     DAIASHGVDAAIERAIKCVEAGADAIFAEAAYDLPTYDKFVRAVKVPVLANITEFGKT
                     PLFTVDELKSVNVGMVLYPLSAFRAMNKAAEAVYTAIRRDGHQKNVLDTMQTRDELYD
                     RIGYHEFESKLDALFQQGKSK"
     gene            complement(6110..7099)
                     /locus_tag="VGC69_08875"
     CDS             complement(6110..7099)
                     /locus_tag="VGC69_08875"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY0295427.1"
                     /translation="MSKPAMRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQKALKGVMMELDDCAFPLLQEMTAHSDPMTAFKDIDVALLVGARPRGPGMERK
                     DLLHANAQIFTAQGKALNAVAKRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLR
                     LDHNRALSQLSAKSGKPVADIESLIVWGNHSPTMYPDTRFTTVAGQKIDAIINDQDWN
                     HGTFIPTVGKRGAAIIDARGLSSAASAANAAIDHVRDWVLGSNGKWVTMGIPSDGSYG
                     IPEGIIYGFPVTTAGGEYTLVKDIEIDAFSREKMDFTLKELLEERDGVKDLLK"
     gene            7377..8183
                     /locus_tag="VGC69_08880"
     CDS             7377..8183
                     /locus_tag="VGC69_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0295428.1"
                     /translation="MTEARPEPLVRAARPAAEGGVSLASGRGEGASFSPLYRQIKALL
                     VQSLERGEWKPGEIIPSEIDLAARFQVSQGTVRKAIDELAAEHMLLRRQGKGTFVATH
                     HEARVRYRFLRLAPDEEGEGGRAESRILECRRVRASAEIARVLELRAGETALMIRRVL
                     SFNAMPTVVDEIWLPGAVFRGLTLDLLVANRAPLYGLFETQFGVSMVRADEKLRAVAA
                     SGEIAGLLGIAAGTPLLQVERVSYTYGDRPMEVRRGTYLTNHYHYRNSLN"
     gene            8326..8751
                     /gene="sdhC"
                     /locus_tag="VGC69_08885"
     CDS             8326..8751
                     /gene="sdhC"
                     /locus_tag="VGC69_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY0295429.1"
                     /translation="MTDSAAQNVKPARPEFRNIGLSDIRHYRLPLPGRLSILHRVSGA
                     LLFLCLPLVIVPLFGASVSSPEGYASVAKCVGYIPVKIVLLILIWGFLHHFCAGIRFL
                     ILDLHYGIDKDSARKSAGVVFIVSLVLTLIFALKLFGAL"
     gene            8751..9131
                     /gene="sdhD"
                     /locus_tag="VGC69_08890"
     CDS             8751..9131
                     /gene="sdhD"
                     /locus_tag="VGC69_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY0295430.1"
                     /translation="MAASNNYGPKRLVVGAHYGILDFIAQRVTAILMAVYTLILVIGL
                     LVMPFSYESWKALFTFHVYTLPVGQLLATLAFFALAWHAWIGVRDIWMDYVKPVGVRL
                     LLQVLTLLWLIGSVVYFAQILWRI"
     gene            9135..10913
                     /gene="sdhA"
                     /locus_tag="VGC69_08895"
     CDS             9135..10913
                     /gene="sdhA"
                     /locus_tag="VGC69_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY0295431.1"
                     /translation="MVAVMNSLPRRQFDVVVVGAGGAGMRCSLQLAQAGLSVAVLSKV
                     FPTRSHTVAAQGGVSASLGNMSEDNWYWHMYDTVKGSDWLGDQDAIEFMCREAPNAVY
                     ELEHFGMPFDRNPDGTIYQRPFGGHTANFGEKPVQRACAAADRTGHALLHTLYQRNVA
                     ARTHFFVEWMALDLLRNEAGDVLGVTALEMETGDIYILEAKTTVLATGGAGRIWAAST
                     NAFINTGDGLGMAARAGIPLQDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNKDGER
                     FMERYAPTLKDLAPRDFVSRSMDQEIKEGRGCGPDGSYVVLKLDHLGAETINKRLPSI
                     REIAIKFGNVDPIKDPIPVVPTIHYMMGGIPTTVNGQVLTWTGSENKVVNGLYAVGEC
                     AAVSVHGANRLGTNSLLDLIVFGRATGNHIVSSHPERQHQHQSVPEAAVDYSLDRVNQ
                     LETRTSGEKPQDVGNGIRAAMQAHCGVFRTLDLLNKGVGKIDELADQAKHIYFKDKSK
                     VFNTARIEALEIANMTEVARATIKSAASRNESRGAHALDDHPTRDDVNWLKHTLWFSA
                     DGRLEYKPVHLKPLTVESFPPKARTF"
     gene            10927..>11565
                     /locus_tag="VGC69_08900"
     CDS             10927..>11565
                     /locus_tag="VGC69_08900"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY0295432.1"
                     /translation="MTKRIIKFEIYRYDPDKDERPYMQKLEVELQPTDKMLLDAIVRI
                     KNEVDDSLAIRRSCREGVCGSDAMNINGKNGLSCTTNLNDLTQPIVLRPLPGLPVIRD
                     LIVDMTHFFNQYHSIRPYLINDTPPPERERLQTPEAREELDGLYECILCACCSTSCPS
                     FWWNPDKFVGPAGLLQAYRFIADSRDEATGSRLDNLEDPYRLFRCHTIMNCVD"
BASE COUNT         2027 a         3762 c         3719 g         2057 t
ORIGIN      
        1 atccgcagac catcgcctac ctcaagctca cgggccgcga ggacgagcag gtcaagctgg
       61 tcgaaaccta tgccaggacg gccggcctgt gggccgacag cttgaagaac gtgcaatacg
      121 agcgcgtgct gagcttcgac ctgtccagcg tggtgcgcac gctggccggg ccgtccaacc
      181 cgcaccgccg cctgcccgtg tccgacctgg ctgcgcgcgg catctcgggt atcgtcgaaa
      241 acacgccggg ccagatgccc gacggcgcgg tcatcatcgc cgccatcacc agctgcacca
      301 acaccagcaa cccgcgcaac gtgatcgcgg cgggcctgct ggcgcgcaac gccaaccgcg
      361 ccggcctcac gcgcaagccc tgggtgaaga gttcgctcgc gcccggctcc aagacggtgg
      421 cgctgtacct ggacgaggcc ggcctgacgt ccgaactgga aaagctcggt ttcggcgtgg
      481 tggcgttcgc ctgtacgacc tgcaacggca tgtccggcgc gctcgacccg aagatccagc
      541 aggaaatcat cgcccgcgac ctctacgcca ccgccgtgct ctcgggcaac cgcaacttcg
      601 acggacgtat ccacccctat gccaagcagg ccttcctggc ttcgccgccg ctggtggtgg
      661 cctacgccat cgccggcacc atccgcttcg acatcgagcg cgacgtgctg ggcgtggacg
      721 cggccggcaa ggaaatccgt ctcaaggata tctggccgag cgatgaggaa atcgacgccg
      781 tggtcgccgc ctcggtcaag cccgagcaat accgcaaggt ctacgacgtg atgttcgccg
      841 tccatgacca gcgcggcgag gcggtgagcc cgctctacgc ctggcggccg cagagcacct
      901 acatccgccg cccgccctat tgggaaggcg cgctggccgg cgagcgctcc ctgcggggca
      961 tgcggccgct ggcgctgctg ggcgacaaca tcacgaccga ccatctgtcg ccgtcgaacg
     1021 ccatcctgcc cgacagcgcg gccggcgaat acctggcgaa aatgggcttg cccgaagaag
     1081 acttcaactc ctacgccacg caccgcggcg accacctcac ggcccagcgc gccacgtttg
     1141 ccaaccccac gctcgtcaac gagatggccc tggccgccga cggcagcgtg aagaagggtt
     1201 cgctcgcccg cgtcgaaccc gaaggcaaga ccatgcgcat gtgggaagcc atcgaaacct
     1261 atatgcagcg caagcagccg ctcatcatcg tggcgggcgc cgactacggc cagggctcgt
     1321 cgcgcgactg ggccgccaag ggggtgcgcc tggcgggcgt cgaggccatc gcggccgagg
     1381 gcttcgagcg catccaccgc accaacctca tcggcatggg cgtgctgccg ctggaattca
     1441 agcccggcgt caatcgcaag acgctggggc tggacggtac cgaaacctac gacgtcgtgg
     1501 gcgagcgcaa gccgcgcgcg gacctgacgc tggtcatcca tcgcaagaac ggcgagacct
     1561 tgcaggttcc cgtcacgtgc cgactcgata cggccgaaga ggtgtccatt tatgaagcgg
     1621 ggggcgtgct gcaacgtttt gctcaagact ttcttgaggc ggcacgcgcg taattcgtgt
     1681 ttttaagccg ccgcggctgc cctgcggaat gccgatgctc ggcgctggaa agcgcctgcg
     1741 cttcggatcc ctccggaccg ccgcggctca cagcatccaa cattgatcga ggcatagcca
     1801 tgactcacgc tccccaagtc cgcattcccg ccacctatat gcgtggcggc accagcaaag
     1861 gcgttttctt caagctcacc gacctgcccg aggcggcccg cgtgccgggt gccgtccgcg
     1921 acgcgctgct gatgcgcgtg atcggcagcc ccgaccccta cggcaagcag atcgacggca
     1981 tgggcgcggc cacgtccagc accagcaaga cggtcatcgt cggcaagagc acccgcaagg
     2041 accacgacgt cgactacctg ttcggccagg tggccatcga ccgggccttc gtggattgga
     2101 ccggcaactg cggcaacctc acggccgccg tcggcccctt cgccatcagc aacggcctga
     2161 tcgattccgc gcgcgtgccc aagaacggcg tggccgtgat ccgcatctgg caggccaata
     2221 tcggcaagac catcattgcc cacgtgccca tgaccgacgg catggtgcag gaaaccggcg
     2281 acttcgaact cgatggcgtg acctttccgg ccgccgaggt gccgctcgaa ttcatggccc
     2341 cggccgacga cggcgaggac ggcggcgcca tgttccccac cggcaacctg gtggacaagc
     2401 tggaagtgcc ggacgtgggc actttcaatg ccacgatgat caatgccggc atcccgacca
     2461 tcttcctgaa cgcccaggac ctgggctaca ccggcaccga gctgcagggc gacatcaacg
     2521 gcgacccgca agcgctcaag cgcctcgaga ccatccgcgc ctacggcgcg ctgcgcatgg
     2581 gcttgatcaa gtcgctcgac gagatcgccg gccggcagca cacgcccaag gtggcgttcg
     2641 tcgcgccgcc ggcaagctat acagcctcca gcggcaagaa gatcgacgcc aaggatatcg
     2701 acgtgctggc gcgcgccatg tccatgggca tgctgcacca cgcgatgatg ggcacggcgt
     2761 cggtcgccat cgccacggca gcggccgtgc ccggcaccct ggtcaacctg gccgcgggcg
     2821 gcggcgaacg caacgccgtg cgcttcggcc acccttcggg cgtgctgcgc gtgggcgccg
     2881 aagccaggct ggtcgacggc caatgggtcg tcaacaaagc gctgatgagc cgcagcgcgc
     2941 gggtgatgat ggaagggtgg gtaagggtgc cgttggagac gattgtctga gtaggtccga
     3001 ctcccaggtt gccggccatg cgaactgtcg ccaagcatgg ttccttagcc ggcagcaagg
     3061 tcacgcacag gtaaatccac gactgcgggt tttgcgcata gacgggattg gtgtgcctgc
     3121 cgatactgcc agctttcttt gggcatgtgg gcaaagcgtt ttgtcctggt gtgaaacgcc
     3181 cattggaata tgtattttta tacatataat tgagggcgca ggcatttcat gcgtttcact
     3241 tggcacgagc ccaagcggca gtcgaaccta aaaagcacgg gtcgggtttc tggagacagg
     3301 catcgtctcc atcagccaca cggaaactga cgcggaaatt cacgttatct ccatgcgcaa
     3361 ggcgactaca gatgaaatcg aaatcctcag cctctacctc caggtccacg ccgcctcggt
     3421 tgcgggtcgg tctgaaggac gtcaagcaag agggctttgc tgccgcagtg caggaaaagc
     3481 tgggcaagca gcgtgtttcc atcatgctgg atacttcggt catcgccttt ttcaaggcca
     3541 aggccggtga tcggggctat cagacgctca tcaaccaggc cttgcatcag gccatgctgg
     3601 gtgagcaatt ggaggccacg ctgcgtcgcg tcatccgtga ggaactgcat ccggcatgag
     3661 cgaccgcgcg ttggcgaccg tcccaaaacc catacgcccg cgcaaggcgg gcgtatgcag
     3721 cacggttttt tacagttctt acttgcgctt ctcgatcgac acgaacttct gatcttccgg
     3781 tccgatgtag ttggccgtcg gccggatgat cttgttgtcc acgcgctgtt cgatgatgtg
     3841 cgccgaccag ccggccgtgc gcgcgatcac gaacaagggc gtgaacatcg ccgtgggcac
     3901 gcccatcatg tgatagctca ccgccgagaa ccagtccagg ttggggaaca tcttcttgat
     3961 gtcccacatc accgtctcca ggcgctccgc gatgtcgaac agcttggtgc tgccggcctg
     4021 cttggagagc ttgcgcgaga cttccttgat gaccttgttg cgcgggtcgg acacggtgta
     4081 gaccgggtgg ccgaagccga tgatgacttc cttgttctcg acgcgctggc ggatgtcggc
     4141 ctcggcttcg tcgagggtgt cgtagcggct ctgcacctcg aaggccactt cgttggcgcc
     4201 gccgtgcttg ggcccgcgca gcgcgccgat gccgccggtg atggccgagt acatgtccga
     4261 gcccgtgcca gccacgacgc ggcaggtgaa ggtggacgcg ttgaattcgt gttcggcgta
     4321 gaggatgagc gaggtgtgca tggctcgcac ccaatcctcg gagggcttct tgccgtgcag
     4381 caggtgcagg aagtggccgc cgatgctgtc gtcgtcggtc tgcacgtcga tcacgcggcc
     4441 gttgtggctg tagtggtacc agtacagcag ggccgagccc aggttggcca tcaggcggtc
     4501 ggcgatgtcg cgcgcgccgg gaatgttgtg gtcgtctttc tcgggcagca cgcagcccag
     4561 caccgacacg gcggtgcgca tcacgtccat ggggtggctg gcggcgggca gggcttccag
     4621 cgcgtcctgc aacggagcgg gcaggccgcg caggctgcgc agcttttcct tgtaggcctt
     4681 gagctcggcc ttgttgggca gcttgccgtg caccagcagg tgggcgattt cctcgaactc
     4741 ggcgtcgctg gcgaaatcga ggatgtcata gccgcggtag tgcaggtcgt tgccggtgcg
     4801 gcccaccgtg cacagggcgg tattgccggc cacgacgccg gacagggcga ccgacttctt
     4861 gggcttgaag ccggagggcg cggcctcggg ggcgggggtt gccgcggcgg cttttttctt
     4921 gggtgccgtg ctcatgtctc actactcctt gtttgggaag gaaatggcac ggccgttccg
     4981 ctgcgtggat gccggcgtgc cgctcgattc acagcttact acttggactt gccctgttgg
     5041 aacagggcgt cgagcttcga ttcgaattcg tggtagccga tgcggtcgta gagttcgtcg
     5101 cgggtctgca tcgtgtcgag cacgttcttc tggtgcccgt cgcgacggat ggcggtgtag
     5161 acggcttcgg cggccttgtt catggcgcgg aaggccgaca gcgggtagag caccatgccc
     5221 acgttcacgc tctttaactc atccacggta aagagcgggg tcttgccgaa ctcggtgatg
     5281 ttggccagca ccggcacctt caccgcgcgc acgaacttgt cgtaggtggg caggtcgtag
     5341 gcggcctcgg cgaaaatggc gtcggcgccg gcttccacgc acttgatggc gcgctcgatg
     5401 gcggcatcga cgccgtgcga ggcgatggcg tcggtgcggg ccaccagata gaagtcgcta
     5461 tcggtgcgcg cgtcggcggc ggccttgacg cggtcggcca tttcctcggt ggagacgatc
     5521 tccttgccgg gacgatggcc gcagcgcttg gcgccgacct ggtcctcgat gtggcaggcc
     5581 gcggcgccgg acttgatcag gctcttgatg gtgcgggcga tgttgaaggc cgaggggccg
     5641 aagcccgtgt cgatgtcgac cagcaggggc aggtcgcaca cgtcggtgat gcggcgcacg
     5701 tcgatgagca cgtcgtccat cgtgttgatg cccaggtcgg gcaggcccag ggagccggcg
     5761 gcgacgccgc cgcccgacag gtagatggcc cggtagccgg cgcgcttggc cagcagggcg
     5821 tggttggcgt tgatcgtgcc gatgatctgc aggggttgtt cttcggcgag cgcctggcga
     5881 aacagggcgc cgggtgaggc ggggcgagtc atgtcttgtc tccgtgctac ggatattttt
     5941 aaaggccgtc gctttgtggc tgtcttgagc cgagggtccc gaaggaatcc gaagcgcagc
     6001 cccttttcag gggcgagcag cggaattgcg cagggcagcc tcggcggcta aagaacgcaa
     6061 aaagcgcgaa aaaaaacggc gaccaaggcc gccgtctttt ttcatctgct tacttcagca
     6121 ggtctttcac gccgtcgcgc tcttcgagca gctccttgag cgtgaagtcc atcttctcgc
     6181 gcgagaaggc gtcgatctcg atgtccttga ccagcgtgta ctcgccgccg gcggtggtca
     6241 cggggaagcc gtagatgatg ccttcgggga tgccgtagga accgtccgag gggatgccca
     6301 tggtgaccca cttgccgttc gaacccagca cccagtcgcg cacgtggtcg atggcggcgt
     6361 tggcggccga ggcggccgac gacaggccgc gggcgtcgat gatggcggcg ccgcgcttgc
     6421 ccacggtggg gatgaaggtg ccgtggttcc agtcctggtc gttgatgatc gcgtcgatct
     6481 tctggccggc cacggtggtg aagcgggtgt cggggtacat cgtgggcgag tggttgcccc
     6541 acacgatcag gctctcgatg tcggccaccg gcttgcccga cttggccgac agttgcgaca
     6601 gggcgcggtt gtggtccagg cgcagcatgg cggtgaagtt cttggcgggc aggtcgggcg
     6661 ccgacttcat ggcgatgtag gcgttggtgt tggcggggtt gccgacgacc agcaccttga
     6721 cgtcgcgctt ggccacggcg ttcagggcct tgccctgggc cgtgaagatc tgggcgttgg
     6781 cgtgcagcag gtccttgcgc tccatgccgg ggccgcgggg acgggcgcca accagcaggg
     6841 cgacgtcgat gtccttgaac gcggtcatgg gatcgctgtg ggcggtcatt tcctgcagca
     6901 gcgggaaggc gcagtcatcc agttccatca tgacgccctt gagggctttc tgcgcttttt
     6961 cgtcggggat ttcgagcagt tgcaggatca ccggctggtc tttgccgagc atttcgccgg
     7021 aggcgatgcg aaacagcagg gcgtaaccga tctggccggc ggcgccggtg acggcgactc
     7081 gcatggcggg cttggacatg aaaattctcc gtgatgtaag aaaggagggc tggaaagacg
     7141 ctgggccttt cagtttaatc ggtgcggccg gcctcgaact tggcaaaaat attgcacaag
     7201 cgttcgccgg gcccgttcga gcatcctaga tcggattagg gtttgcgtca attgatctta
     7261 tatcttatat aagacataag acgttggaca gcggccgtat ccatgtgcga caatgcctcg
     7321 ctcattcacc gccagcttcc aaccggccga tgggcatgaa atcaaaacgc agacccatga
     7381 cagaagcccg gcccgaaccc ctggtgcgcg ctgcccgacc ggcagccgag gggggcgttt
     7441 cgctcgcctc gggacgcggt gaaggggctt cgttcagccc gctctaccgg cagatcaagg
     7501 cgctcctggt acaaagcctg gagcgcggcg aatggaagcc tggcgagatc atccccagcg
     7561 aaatcgatct ggcggcccgt ttccaggtca gccagggcac ggtgcgcaag gccatcgatg
     7621 aactcgccgc cgagcacatg ctgctgcgcc gccagggcaa gggcactttc gtggccacgc
     7681 accacgaggc gcgcgtgcgc taccgtttcc tgcgcctggc gcccgacgag gaaggcgagg
     7741 gcgggcgcgc cgaaagccgc atactcgaat gccgccgcgt gcgcgcctcg gccgagatcg
     7801 cccgcgtcct cgagctgcgg gccggcgaga ccgcgctcat gatccgccgc gtgctgtcgt
     7861 tcaacgccat gccgacggtg gtcgacgaga tctggctgcc cggcgccgtg ttccgcggcc
     7921 tcaccctgga tctgctggtc gccaaccggg cgccgctcta tgggttgttc gagactcagt
     7981 tcggcgtcag catggtgcgt gccgacgaga agctgcgggc cgtggcggcc agcggcgaaa
     8041 tcgccgggct gctcggcatt gcagcgggca cgccgctgct gcaggtcgag cgcgtctcct
     8101 atacctatgg cgaccggccc atggaagtcc ggcgcggcac atacctgacc aaccactatc
     8161 actatcgcaa tagcttgaat tgatggattt ttttatcggc gtttgaaaca actcaccacc
     8221 tgggtgaaaa taccgcgatt tccccgatgg ggaacgggcg catccgccgc gaagattttg
     8281 ctttaccaaa aacctagcca taaacccaga aacaccgagg tcgtcatgac tgactcagct
     8341 gctcagaacg taaagccagc gcgtcctgaa tttcgcaata tcggcttgtc cgacattcgc
     8401 cattaccgcc tgcctctccc gggccggctg tccattctgc atcgcgtcag cggcgcactg
     8461 ttgttcctgt gcctgcccct ggtcatcgtg ccgctcttcg gcgccagcgt gagttcgccc
     8521 gagggctacg ccagcgtggc caagtgcgtg ggctacatcc ccgtcaagat cgtcctgctg
     8581 atcctgatct ggggcttcct gcatcacttc tgcgcgggca tccgcttcct gatcctcgac
     8641 ctgcactacg gcatcgacaa ggattcggcg cgcaagagcg ccggcgtggt gttcatcgtc
     8701 agcctggtgc tcacgctgat tttcgctctc aaactattcg gagctctgta atggcagcca
     8761 gcaacaacta cggcccgaag cgtctcgttg tcggcgcgca ttacgggatt ctcgacttca
     8821 tcgcccagcg cgtcacggcc atcctcatgg cggtgtatac gctgatcctg gtcatcggcc
     8881 tgctggtcat gcccttctcg tacgagagct ggaaggcgct gttcacgttc catgtgtaca
     8941 ccctgcccgt gggccagctc ctggccactc tcgccttctt cgcgctggcc tggcatgcct
     9001 ggatcggcgt gcgcgacatc tggatggact acgtcaagcc ggtgggcgtg cgcctgctgc
     9061 tgcaggtcct gaccctgctg tggctgatcg ggtcggtcgt ctatttcgcg caaatcctct
     9121 ggagaatcta agccgtggtc gctgtcatga attccctgcc gcgccgccag tttgatgtgg
     9181 tggtcgtggg cgccggcggt gccggcatgc gttgttccct gcaattggcc caggcgggcc
     9241 tgtcggttgc cgtgctgtcc aaagtgtttc ccacccgttc gcatacggtg gcggcgcagg
     9301 gcggcgtgag cgcctcgctg ggcaatatga gcgaagacaa ctggtactgg cacatgtacg
     9361 acaccgtgaa ggggtcggac tggctgggcg accaggatgc catcgagttc atgtgccgcg
     9421 aagcgcccaa cgccgtctac gagctcgagc acttcggcat gcccttcgac cgcaaccccg
     9481 acggcacgat ctaccagcgt ccgttcggcg gccacaccgc caacttcggc gagaagccgg
     9541 tgcagcgcgc ctgcgccgcc gccgaccgca ccggccacgc cctgctgcac acgctctacc
     9601 agcgcaacgt cgcggcccgc acccacttct tcgtggagtg gatggcgctc gacctgctgc
     9661 gcaacgaagc cggcgacgtg ctcggcgtga ccgccctgga aatggaaacg ggcgacatct
     9721 acatcctcga agccaagacc accgtgctcg ccacgggcgg cgccggccgc atctgggccg
     9781 cgtccaccaa cgccttcatc aacaccggcg acggcctggg catggcggcg cgcgccggca
     9841 tcccgctgca ggacatggag ttctggcaat tccaccccac cggcgtggcc ggcgcgggcg
     9901 tgctgatcac cgaaggcgtg cgcggcgaag gcggcatcct gctcaacaag gacggcgaac
     9961 gcttcatgga gcgctatgcg cccacgctga aggacctggc gccgcgcgac ttcgtgtcgc
    10021 gctcgatgga ccaggaaatc aaggaaggcc gcggctgcgg tcccgatggc agctacgtcg
    10081 tgctcaagct cgaccacctg ggcgccgaga ccatcaacaa gcgcctgccc tcgatccgcg
    10141 aaatcgccat caagttcggc aacgtcgatc ccatcaagga cccgatcccg gtcgtgccca
    10201 ccatccacta catgatgggc ggcatcccca ccaccgtgaa cggccaggtg ctcacctgga
    10261 cgggcagcga gaacaaggtc gtcaacggcc tgtacgccgt cggcgagtgc gccgcggtgt
    10321 cggtgcacgg cgccaaccgc ctgggcacca actcgctgct cgacctgatc gtgttcggcc
    10381 gcgccaccgg caaccacatc gtcagctcgc atcccgagcg ccagcaccag caccaatcgg
    10441 tgcccgaggc ggcggtcgac tattcgctgg accgcgtcaa ccagctcgaa acccgcacct
    10501 cgggcgaaaa gccccaggac gtcggcaacg gcatccgcgc ggccatgcag gcccactgcg
    10561 gcgtgttccg cacgctggac ctgctcaaca agggcgtggg caagatcgac gagctggccg
    10621 atcaggccaa gcacatctac ttcaaggaca agtccaaggt gttcaacacc gcgcgcatcg
    10681 aggcgctgga aatcgccaac atgactgaag tggcccgcgc cacgatcaag tcggcggcca
    10741 gccgcaacga aagccgcggc gcgcatgcgc tcgacgacca cccgacccgc gacgacgtga
    10801 attggctcaa gcacaccctg tggttctcgg ccgacggccg cctggaatac aagcccgtgc
    10861 atttgaagcc cctgaccgtc gagtccttcc cgcccaaggc gcgtactttc taagcaggat
    10921 cacgcaatga ccaagcgtat catcaagttc gaaatctacc gctacgatcc cgacaaggac
    10981 gagcgtccct atatgcagaa gctggaggtc gaactccagc ccaccgacaa gatgctgctc
    11041 gacgccatcg tgcgcatcaa gaacgaagtc gacgacagcc tggcgatccg ccgctcctgc
    11101 cgcgaaggcg tgtgcggttc cgacgccatg aacatcaatg gcaagaacgg cctgtcgtgc
    11161 accaccaacc tcaacgacct gacccagccc atcgttctgc ggccgctgcc cggcctgccc
    11221 gtgatccgcg acctgattgt ggacatgacg cacttcttca atcagtacca ctcgatccgt
    11281 ccgtacctca tcaacgacac gccgccgccc gagcgcgagc gcttgcagac gcccgaggcc
    11341 cgcgaagagc tcgacggcct gtacgagtgc atcctgtgcg cctgctgctc gacgtcctgc
    11401 ccgtcgttct ggtggaaccc ggacaagttc gtcggcccgg ccggcctgct gcaagcctac
    11461 cgcttcatcg ccgacagccg cgacgaagcc accggctcgc gcctggacaa cctggaagat
    11521 ccgtaccgcc tcttccgctg ccacaccatc atgaactgcg tcgat
//
LOCUS       DASWPO010000162        15071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1108450, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000162 DASWPO010000000
VERSION     DASWPO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108450"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..814
                     /locus_tag="VGC69_08905"
     CDS             56..814
                     /locus_tag="VGC69_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY0295433.1"
                     /translation="MSSSISSGRVVNIAAYQFVTLDALAVLREQVRERALALGLKGTV
                     LLAPEGINLFLAGTAEAIEGFLRWLRGDGRFADLQAKYSESATVPFGKMLVKIKREII
                     RMDHPAIRPESGRASSVDGPTLARWLEQGRDDDGREVLMLDTRNAFEVDVGTFEGAVD
                     WRISRFTQFPAAVRAHLGELQDKTVVSFCTGGIRCEKAAIYMAEAGVPHVYQLEGGIL
                     KYFEETGGVGFRGNCFVFDERMSLDPALLPARDR"
     gene            complement(860..1642)
                     /locus_tag="VGC69_08910"
     CDS             complement(860..1642)
                     /locus_tag="VGC69_08910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0295434.1"
                     /translation="MASQSPVRIGYAANFTAPCHAWLQKRLFTHLLPDNFGKTHMSLR
                     MAEISKQLRKIRSIDRHAPAHDARGKVSGLRNFSWQASASNVMVVAPSASTPTRIWPI
                     ERYAEVINHLYRSRGIKPVIIGGPADKAVCRQLLGLIQNTEAVDMAGQLSLVESLALI
                     GTSRLLLTNESGPMHMGRISNAPTVVLASGADFTNYLQYPDPHSNYRVVTADDKSCFD
                     CSWHCIYKSPPGQVIKPCLNAVGGGQALAAIESLLETTATSA"
     gene            1523..2182
                     /locus_tag="VGC69_08915"
     CDS             1523..2182
                     /locus_tag="VGC69_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295435.1"
                     /translation="MRLAEVVRQEVGEQPLLKPGMAWRGEVRRIAYSDGALAGQGHGV
                     AQFGAGQVGVDPDVESRLPDQVQECEFPGKMLSDGLGVDDVQMVGQVDDAGDLGVLPF
                     GKKRDFRGGSAVCDALQRFVEEDPIPDSAALDDAYPGLAGEGAALLFEKQVRCKQGKR
                     ASGAFDLVEGGLLYFHAGSWGVSLDSVADRLDASPMRLENANCYRTRIPGVAPGNGSS
                     T"
     gene            complement(2131..4239)
                     /gene="metG"
                     /locus_tag="VGC69_08920"
     CDS             complement(2131..4239)
                     /gene="metG"
                     /locus_tag="VGC69_08920"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY0295436.1"
                     /translation="MSSRTLFVTTALPYANGSFHIGHIMEYIQADIWVRAMRMAGHTV
                     HFVSADDAHGAPIMLKAEKEGITPQQLVARYAAERPQYLDGFHVRFDHWHSTDTPENV
                     ELSQDIYRALQAQDLIDTRVIEQFYDPVKGMFLADRYIKGECPKCHSKDQYGDSCEVC
                     GSAYAPTDLINPYSSLTGATPVLKSSEHFFFKLSDPRCVAFLREWTAASNAQGTPHLQ
                     SEMFGKTREWLGSDGGESSLGDWDISREAPYFGIEIPGAPGKYFYVWLDAPVGYLASL
                     KAYCALKGLDFDALLDPKGHTEQVHFIGKDIIYFHALFWPAMLKFAGRKTPDAINVHG
                     FITVSGEKMSKSRGTCISPLRYLEIGMNAEWLRYYLAAKLNAKVEDLDFNPEDFIARV
                     NSDLVGKYINIASRAANFVTKYFDGALGYLETAQGGTAALSAEYAAAAEAARAAFEAR
                     EYNRAIREIMALADRINQAFDAAQPWVLAKDFANADAGRQAALQDICSRALAGFKALS
                     VMLAPVLPALARRVAHELFGGGADFVWADAGKLPERVAPFKHLMQRVEPKMLDDLFEP
                     PAEAASEAPAAESAAPPGGESLAETISIDDFAKVDLRIARIVNCEHVEGSTKLLRLTL
                     DVGEGRHRNVFSGIKSAYEPADLIGKLTVMVANLAPRKMKFGVSEGMVLAASHADEHA
                     NPGIYVLEPFPGATPGMRVR"
     gene            complement(4412..4690)
                     /locus_tag="VGC69_08925"
     CDS             complement(4412..4690)
                     /locus_tag="VGC69_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295437.1"
                     /translation="MHDFLPRLLRNVFIALIALIGMFMALIFMVSTAVAVGLLYIAAK
                     LRGKPFGMRAYWQRRQAQHPGVPPFAAPGQPYAKAATDVIDVEAREIR"
     gene            4811..5896
                     /gene="apbC"
                     /locus_tag="VGC69_08930"
     CDS             4811..5896
                     /gene="apbC"
                     /locus_tag="VGC69_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEY0295438.1"
                     /translation="MSITIEQIRAALRTAHDPYTRLPLAASVKDRDIQAGAAGLAVHI
                     ELGYPAGHAQADVAGLAVQALAAAGAPGAQVTVGWKVGPHAVQSTLKPLSTVRNIIAV
                     ASGKGGVGKSTTAVNLALALAADGARVGVLDADIYGPSVPLMLGLKGKPVSHDNKSMQ
                     PLQSHGIEANSIGLLIEPDSPMIWRGPMATQALEQLLRQTNWGDLDYLLIDMPPGTGD
                     IALTLAQKVPVVGAIIVTTPQDIALLDARKGLRMFEKVDVPVLGVVENMAMHICTQCG
                     HVEHIFGEGGGKRMAEQYGVAWLGSLPLAMSIREQADSGAPTVAAHPDSEAAGLYRGI
                     ARKVAALVAALPRDMAGKLPSVVVQPG"
     gene            5950..7692
                     /locus_tag="VGC69_08935"
     CDS             5950..7692
                     /locus_tag="VGC69_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY0295439.1"
                     /translation="MACAKAPLVEVDAGDAQPGAVQGIVEAVTRAVGAIARLADDQDA
                     DESARLQRRADEAARSALATQGYFTPRVTLSPGQDAQGGPVWRIAVAPGVRAEVESVD
                     LRFAGRVTRPEYADRIAAWRRDWALPAGRPFVNADWSKAKSDLLRAVSGQDFPTARIT
                     SSSADVDPQSARVRLVVAIDSGPRVRLGALQVDGLRRVPRKLVEGTVRYAPGEAYEQD
                     KLDHWQQALQSTAFFRGAFVSLDTSKLPPAPDDDGDFTLPVTVRVTEAAPRRAAVSVG
                     VDDASGARLESTYRQNVVFGQPVTLSAGFGVDRLRQRAYMDFLLPPDADGDKDSIGVL
                     ADRSDVQGLQVTRFALGATRLQERHACCGGRAEYETRWGALLAHDHVKIDGGETYDLP
                     TATVTVEWLRRDVDKKYDPREGNLIALGGGVGMALDTGRPYTRAHLRAQKWWPVAKLD
                     SLTLRGEVGRVWADARTTVPDDFGFRTGGARTVRGYSYLSLGEKRDNAVVGAPALAVA
                     SIEYDHYFNYRWGMGVFVDAGDAAASFSSLHPAVGYGVGARVRTPAGPLFLDVAYGQR
                     DHQLRLHFSLGIAF"
     gene            7701..10967
                     /locus_tag="VGC69_08940"
     CDS             7701..10967
                     /locus_tag="VGC69_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0295440.1"
                     /translation="MALLRAVLHWLLVWLLPGLAMLAALCLACALWLLGSQAGAQLLL
                     PGALELLGGHAEAVQGSVLRGLRVGRLQAAFGGTEVDVEGLALEVDWRALLARRAHVR
                     LLAADRLRIVLAGAAAGAGPDDAPMAPITLPVAVALDRLELGSFELVRDGQPLPVSLD
                     GLSASLAADADGARLRVDGLRAGHAQEQAQVQGSVELGPQPWSAQSLRALALDLSREG
                     GQDLSLRVGRQALDARTDRLAGTLRASRFDLGSLLGPGLPQALLDLQADFEADVAGLQ
                     TLRRLHASARIGGDSRWNGQALSGTLEAGIRALDAPADPQAPLPGWALDAAQADLRLG
                     ASRISLRGQADTHQGQLALEANIPALAAFWPGLPGGARLQARVEGTAASHRLTAQASY
                     ASGHAQAGRLGMAPLRLDVAAQGGWGPGPDGAPTGWRGTVSRLSADSAGFSVALGRPA
                     PLAWLPGAQAPQWGIQVGATTLSLAFPGGQRLVLSHAGSRLGGGRWETAGRGDGLALT
                     TDMMNQLHRALGAPVRAGGDAAGRGAAPSQRVVLDASWNLRFDGALAGQARIERRAGD
                     LLIPGSPPISLGLRGLTLSVAATPTGPATSRLDASLDLRTEKMGTLQARASAMLAALA
                     LDARQPVRTSLDADIGDLAWLGLFTGDAYAFGGALRVQAQAQGTPQGKWSAQGSLHGT
                     GLRMLRLEDGVQLVDGTLDAHLDGDRFVLDALRFPASRQVMPQDARTRDWVEHDPDAQ
                     GGDVEASGHWDLAESRGQVDVRLHRFPALQRADRYAMLSGALRLDAELPRLRLTGDLT
                     ADAGWASVDILQGVPTLDDDVHVARPGQESAAPATPLQFDMDIGVNAGPRFFLTGMGL
                     DAGLQGSLRIVMQDGQLRGTGELRTRAGTFQAYGQKLQLRRGIVTFQGRLDNPLLDIE
                     AVRTDIQQVQAGVRVAGTAQRPRIDLISTPDMSDVEKLSWLVMGRAPDASGSDTALLL
                     SVGASLLGGDTPFYKQLGLDDVGIRTGTLGSSGSLLPDQTVATNAVDSTSASDLATQF
                     IVASKHFANGITLSVEQAMSGTGTVGRASYRLARGLSLDLKAGEVNGLDLVYRWFLGE
                     "
     gene            11020..11583
                     /gene="dcd"
                     /locus_tag="VGC69_08945"
     CDS             11020..11583
                     /gene="dcd"
                     /locus_tag="VGC69_08945"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY0295441.1"
                     /translation="MSIKSDRWIRQAAQAGMIEPFEAGQVRNVNGERIVSYGTSSYGY
                     DVRCAAEFKIFTNINSTIVDPKNFDEKSFVDFKGDVCIIPPNSFALARTVEYFRIPRS
                     VLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYAGEGCAQFLFLE
                     SDEVCETSYADRGGKYQGQRGVTLPRT"
     gene            complement(11595..12107)
                     /locus_tag="VGC69_08950"
     CDS             complement(11595..12107)
                     /locus_tag="VGC69_08950"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0295442.1"
                     /translation="MPVQIRRLAPDDAPALRDLRLDALSEAPHAFGSSLAEEQAMDLD
                     GFRQWTSGKESALFGAFVGGHLAGMVGMTRQPRSKMRHKAYIWGMYIEPGQRGLGLGG
                     RLLEAALASAAALPGLRQVQLSVNAGNAPAIALYERAGFREYGREPEALCVAGALYDE
                     ILMARPIPRS"
     gene            12297..14558
                     /locus_tag="VGC69_08955"
     CDS             12297..14558
                     /locus_tag="VGC69_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEY0295443.1"
                     /translation="MKFRFPIFIIDEDYRSENASGLGIRALASAIQAEGMEVIGVTSY
                     GDLSSFAQQQSRASAFILSIDDEEFDDSSPEDVASAIKNLRSFIGELRFRNADIPIYL
                     YGETRTSEHIPNDILRELHGFIHMFEDTPEFVGRHIIREAKSYVDGLAPPFFKELVDY
                     AQDGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPI
                     AESELNAARIFHADHCFFVTNGTSTSNKVVWHANVAAGDVVVVDRNCHKSILHAITMT
                     GAIPVFLRPTRNHLGIIGPIPLEEFDPENIRKKIEANPFAREALDKRPRILTLTQSTY
                     DGVIYNVEMIKEKLGTYIDTLHFDEAWLPHASFHEYYQDMHAIGQNRPRSKEAMVFAT
                     HSTHKLLAGISQASQIIVQESETRKLDRNIFNEAYLMHTSTSPQYAIIASCDVAAAMM
                     EPPGGTALVEESIKEALDFRRAMRKVESEFGKNDWWFKVWGPDRLPASGIGSRDDWIL
                     ESNAHWHGFGDLAEGFNMLDPIKATVVTPGLDMSGSFGETGIPAALVSKYLTEHGVVV
                     EKTGLYSFFILFTIGITKGRWNTLLTALQQFKDDYDRNHPLWRILPEFCRDHRGYERL
                     GLRDLCQQIHEAYRAHDVARLTTEMYLSDMVPALKPSDAFARMAHREVERVTIDELEG
                     RVTGVLLTPYPPGIPLLIPGERFNRTIVQYLQFAREFNARFPGFETDIHGLAEDVGPD
                     GETRYYVDCLIED"
     gene            complement(14707..>15071)
                     /locus_tag="VGC69_08960"
     CDS             complement(14707..>15071)
                     /locus_tag="VGC69_08960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0295444.1"
                     /translation="RKVFITPMENGLRMAGTVEFGSLTHPPSMQRALLLGEHARAGLP
                     TVDTRGAATWMGHRPCLPDSMPVLGPVPGRNGLWCAFGHGHLGLTGSANTGKLIAGAM
                     AGQPDAQRILGPFSIERF"
BASE COUNT         2265 a         4921 c         5424 g         2461 t
ORIGIN      
        1 gttcggcaaa tccacgctga tccgggagcg gcgccggccc gattaaaatc cgggcatgag
       61 ttcctccatt tcttccggcc gcgtggtcaa tatcgcggct taccaattcg tcacgctcga
      121 tgcgcttgcc gtgctgcgcg agcaggtgcg cgagcgggcg cttgcgctgg gcctgaaggg
      181 cacggtgctg ctggcgcccg agggcatcaa tctgttcctg gccggcacgg ccgaggcgat
      241 cgagggtttt ctgcggtggc tgcgtggcga cgggcgcttc gcggatctgc aggccaagta
      301 cagcgagtcc gccacggtgc cgttcggcaa gatgctggtg aagatcaagc gcgagatcat
      361 ccgcatggat cacccggcga tccgccccga gtcgggccgc gcgagcagcg tggacgggcc
      421 gacgctggcg cgctggctgg agcaggggcg cgacgacgac ggccgcgagg tgctgatgct
      481 cgatacgcgc aatgccttcg aggtggatgt gggcacgttc gagggggcgg tggattggcg
      541 catttcgcgc ttcacgcagt ttcccgccgc ggtgcgggcg cacctgggcg agctgcagga
      601 caagacggtg gtgagctttt gcaccggcgg catccggtgc gagaaggcag ccatctatat
      661 ggccgaggcg ggggtgcccc atgtctacca gctcgaaggc gggatcctga agtacttcga
      721 ggagaccggc ggcgtggggt tcagggggaa ctgcttcgtg ttcgacgaac ggatgtcgct
      781 ggatcctgcc ttgctgcccg cgcgggaccg gtagcgcgat gcggtgcgat tttgatcccg
      841 ctgccatggc ggccgcgggt caggcgcttg ttgccgtggt ttccagaaga gactcgatgg
      901 cggccagggc ttggccgccg ccgacggcgt tcaggcaggg cttgatgacc tggccgggcg
      961 ggcttttgta aatgcagtgc cagctgcagt caaagcagga cttgtcgtcg gcggtgacga
     1021 cccggtagtt gctgtgaggg tcggggtatt gaaggtagtt cgtaaagtcg gcgcccgagg
     1081 cgaggacgac ggtcggcgca ttgctgatgc gccccatgtg catggggccg ctttcgtttg
     1141 tcaggagcag cctcgatgtg ccgatgaggg ccaggctttc caccagggag agttgcccgg
     1201 ccatgtctac cgcctcggta ttctggatca ggccgagcag ttgccggcaa accgccttgt
     1261 cggccgggcc gccgatgatg acgggtttta tcccgcgcga gcggtacaga tggttgatga
     1321 cttcggcgta gcgttcaatc ggccatatgc gtgtcggggt gctggcgctg ggggcgacga
     1381 ccatgacgtt cgacgcgctt gcctgccatg agaaattccg caggcctgaa accttgcccc
     1441 gggcgtcatg ggccggggca tggcggtcga tggatcgaat cttccgcagt tgcttcgata
     1501 tttccgccat gcgcaaggac atgtgcgtct tgccgaagtt gtccggcagg aggtgggtga
     1561 acagccgctt ttgaagccag gcatggcatg gcgcggtgaa gttcgccgca tagcctattc
     1621 tgacggggct ttggctggcc agggccatgg cgtcgcccag tttggtgcgg gacaggtcgg
     1681 cgtcgatcca gatgtcgaaa gtcgattgcc tgaccaggtt caggagtgcg agtttcctgg
     1741 aaagatgctt tctgatggcc ttggggtcga tgatgtgcag atggtcggcc aggttgatga
     1801 tgccggcgat ctcggcgttc tgcctttcgg caaaaagcgt gatttccgcg gcgggagcgc
     1861 agtttgcgat gctctccagc gtttcgtgga agaggatcca atccccgatt ccgccgcgct
     1921 tgatgatgcc tatcctgggt tggccggaga aggcgccgcc ctgctttttg agaagcaggt
     1981 tcgttgcaag caaggcaagc gtgcgtctgg cgcattcgat ctggtagagg gcggactgct
     2041 ttattttcat gccggatcgt ggggagtcag cctggattcc gtagccgatc ggctcgatgc
     2101 ctcaccgatg cggctcgaga acgcgaattg ctaccgcacc cgcatcccgg gcgtcgcgcc
     2161 cggaaacggt tccagcacat agatgccggg attggcatgc tcgtccgcgt ggctggcggc
     2221 cagcaccatg ccttcggaca cgccgaactt catcttgcgc ggggccaggt tggccaccat
     2281 cacggtgagc ttgccgatca ggtcggcggg ctcataggcc gacttgatgc ccgagaacac
     2341 attgcgatgg cggccttcgc ccacgtccag ggtcaggcgc agcagcttgg tcgagccttc
     2401 gacgtgttcg caattgacga tcctggcgat gcgcaggtcg accttggcga aatcgtcgat
     2461 ggagatggtc tcggccaggg attcgccgcc gggaggcgcg gcagactcgg ccgcgggcgc
     2521 ttcggacgcg gcttcggccg ggggttcgaa caggtcgtcg agcatcttgg gctccacgcg
     2581 ctgcatcagg tgcttgaagg gggcgacgcg ctcgggcagc ttgccggcgt cggcccagac
     2641 gaagtcggcg ccgccgccga agagttcgtg cgccacgcgc cgggccaggg cgggcagcac
     2701 cggggccagc atcaccgaca gggccttgaa gccggccagg gcgcgcgagc agatgtcctg
     2761 cagggcggcc tggcggccgg cgtcggcgtt ggcgaagtcc ttggccagaa cccagggctg
     2821 ggcggcgtcg aaggcctggt tgatgcggtc ggccagggcc atgatctcgc ggatggcgcg
     2881 gttgtactcg cgggcctcga aagcggcgcg ggcggcttcg gcggcggcgg cgtattcggc
     2941 cgagagggcg gcggtgccgc cttgcgccgt ttccagatac cccagggcgc cgtcgaagta
     3001 cttggtgacg aagttggccg cgcggctggc gatgttgatg tacttgccca ccaggtcgct
     3061 gttgacgcgg gcgatgaagt cctcggggtt gaagtccagg tcttcgacct tggcgttgag
     3121 cttggcggcc aggtagtagc gcagccattc ggcgttcatg ccgatttcca ggtagcgcag
     3181 cggcgagatg caggtgccgc ggctcttgga cattttctcg ccgctcacgg tgatgaagcc
     3241 gtgcacgttg atcgcgtcgg gcgtcttgcg gccggcgaac ttcagcatgg cgggccagaa
     3301 gagcgcgtgg aagtagatga tgtccttgcc gatgaagtgc acctgctcgg tatggccctt
     3361 ggggtcgagc agggcgtcga agtccaggcc cttgagcgcg caataagcct tgagcgaggc
     3421 caggtagccg accggggcgt cgagccacac gtagaagtac ttgcccggcg cgccggggat
     3481 ctcgatgccg aaatagggcg cttcgcgcga gatgtcccag tcgcccaggc tcgactcgcc
     3541 gccatccgat cccagccatt cgcgcgtctt gccgaacatc tcggactgca ggtgcggcgt
     3601 gccctgggcg ttgctggcgg ctgtccattc gcgcaggaat gccacgcagc gcgggtcgga
     3661 gagcttgaag aagaagtgct cggacgattt cagcaccggc gtggcgccgg tgagcgagga
     3721 gtaggggttg atgaggtcgg tgggggcgta ggccgagccg cacacctcgc aggagtcgcc
     3781 gtactggtcc ttgctatggc acttggggca ttcgcccttg atatagcggt cggccaggaa
     3841 catgcccttg accgggtcgt agaactgctc gatcacgcgc gtgtcgatga ggtcctgggc
     3901 ttgcagggcg cggtagatgt cctgcgacag ctcgacgttc tcgggcgtgt cggtggagtg
     3961 ccagtggtcg aagcggacgt ggaagccgtc gaggtactgg gggcgttcgg cggcgtagcg
     4021 ggccaccagt tgctgggggg tgatgccttc cttttcggcc ttgagcatga tgggcgcgcc
     4081 gtgcgcgtcg tcggcgctca cgaagtgcac cgtatgcccg gccatgcgca tcgcccgcac
     4141 ccagatgtcg gcctggatgt attccatgat gtggccgata tggaaggagc cgttggcgta
     4201 ggggagggcg gtggtgacga agagcgtgcg tgacgacatg gctgggctgg gctgtctgtg
     4261 aaaaggaaaa agtcccgggg ggagggagcc tcgtatttta gaggttcggc ggtgtttggg
     4321 ttcttgaggc gcatcggcgc ggcggggcct ggaggcgtgg ccgtagggtg ggtggtcccg
     4381 cctctttggg gcgggaccac ccaccgccaa ctcagcgaat ttcgcgggcc tcgacgtcga
     4441 tgacgtcggt ggcggccttg gcgtagggct ggccgggcgc ggcaaagggc gggacgcccg
     4501 gatgctgggc ctggcgccgc tgccagtagg cgcgcatgcc gaaaggcttg ccgcgcagct
     4561 tggcggcgat gtagagcagg cccaccgcga cagccgtgga gaccatgaag atgagcgcca
     4621 tgaacatgcc gatcagggcg atcagggcga tgaacacatt gcgcagcagg cggggaagga
     4681 aatcgtgcat ggcggctggg gtggaaaacc cggcagtagg gggacccggg ggtcgggccg
     4741 gtatatccgc tattcttccg gaatgagcgg cgggcgcgac gaaatgtccg cccatgggaa
     4801 cctggatgtc atgagtataa cgatagagca aattcgcgct gcgctgcgca ccgcgcacga
     4861 cccctacacc cggctgcccc tggcggcttc tgtaaaagat cgtgacattc aggcgggcgc
     4921 ggccgggctg gccgtgcata tcgagctggg ctaccccgcc ggccatgcgc aggccgacgt
     4981 ggccgggctc gccgtccagg cgctggccgc cgccggcgcg cccggcgccc aggtgacggt
     5041 gggctggaag gtcgggcccc acgcggtgca gagcaccctc aagcccctgt ccacggtgcg
     5101 caacatcatt gccgtggcgt cgggcaaggg cggggtgggc aagagcacca cggccgtcaa
     5161 cctggccctg gccctggcgg ccgacggcgc ccgggtgggc gtgctggacg ccgacatcta
     5221 cggccccagc gtgccgctga tgctgggcct caagggcaag ccggtgagcc acgacaacaa
     5281 gtccatgcag cccctgcaga gccacggcat cgaggccaat tccatcgggc tgctgatcga
     5341 gcccgactcg cccatgatct ggcgcggtcc catggcgacc caggcgctgg agcagttgct
     5401 gcggcaaacc aactggggcg acctggacta cctgctcatc gacatgccgc cgggcacggg
     5461 cgacatcgcc ctgaccctgg cgcagaaggt gccggtggtg ggcgccatca tcgtcaccac
     5521 cccgcaggac atcgccctgc tcgacgcccg caagggcctg cgcatgttcg agaaggtcga
     5581 cgtgccggtg ctgggcgtgg tcgagaacat ggccatgcac atctgcacgc aatgcggcca
     5641 cgtggagcac atcttcggcg agggcggcgg caagcgcatg gccgagcagt acggcgtggc
     5701 ctggctgggc agcctgccgc tggccatgtc gatccgcgag caggccgatt cgggcgcgcc
     5761 caccgtggcc gcgcatcctg acagcgaagc cgcgggcctg taccgcggca tcgcgcgcaa
     5821 agtggccgcg ctcgtggccg cgctgccgcg cgacatggcg ggcaagctac cctccgtcgt
     5881 cgtgcaaccg ggctgatgcg ggcgcctgcc cgcatcgtgg cagggtggtg cctgtcggcc
     5941 tgggcctgta tggcctgcgc caaggcgccg ctggtcgaag tggatgccgg cgacgcgcag
     6001 cccggggcgg tgcagggcat cgtcgaggcg gtgacgcggg ccgtgggcgc cattgcccgg
     6061 ctggccgatg accaggacgc ggacgagtcg gcccggctgc aacggcgcgc cgacgaggcc
     6121 gcgcggtcgg cgctggccac gcaaggttat ttcacgccgc gggtgacgct gtcgcccggc
     6181 caggacgcgc aaggcggtcc ggtctggcgg attgccgtgg cgcccggcgt ccgggccgag
     6241 gtcgagtcgg tggacctgcg ttttgccggc cgcgtcaccc gtcccgaata cgccgaccgc
     6301 atcgccgcct ggcgccgcga ttgggccttg ccggcggggc ggcccttcgt caacgccgat
     6361 tggagcaagg ccaagtccga tctgctgcgg gccgtctccg ggcaggactt ccccaccgcg
     6421 cgcatcacgt cctcgtcggc cgacgtcgac ccgcaatcgg cgcgcgtgcg gctggtggtc
     6481 gccatcgaca gcgggccgcg ggtgcgcctg ggcgcgttgc aggtggatgg cctgcggcgc
     6541 gtgccgcgca agctcgtcga gggcaccgtg cgctacgcac cgggagaggc ctacgagcag
     6601 gacaagctcg atcattggca gcaggccttg caatccaccg cctttttccg cggcgccttc
     6661 gtctcgctgg atacatcgaa gctgcctccc gcgcccgatg acgacggcga tttcacgctg
     6721 cccgtgacgg tgcgcgtgac cgaggccgcg ccgcgccgcg cggcggtgtc ggtgggcgtg
     6781 gacgacgcct cgggcgcgcg gctggagtcc acctaccggc agaacgtggt gttcggccag
     6841 ccggtcaccc tgagcgccgg cttcggcgtg gaccggctgc gccagcgcgc ctatatggac
     6901 ttcctgctgc cgcccgacgc ggatggcgac aaggactcca tcggcgtgct ggccgaccgc
     6961 tccgatgtgc aaggcttgca ggtgacgcgc ttcgcgctgg gcgccacgcg cctgcaggag
     7021 cgccatgcct gttgcggcgg ccgggccgaa tacgagacgc gctggggcgc cctgctggcg
     7081 cacgaccacg tcaagatcga cggcggcgag acctatgacc tgcctaccgc cacggtcacc
     7141 gtcgaatggc tgcggcgcga cgtggacaag aaatacgatc cgcgcgaagg caacctgatc
     7201 gcgctgggcg gcggcgtcgg catggcgctg gacaccggcc ggccctatac ccgggcgcat
     7261 ctgcgggccc aaaaatggtg gccggtggcc aagctggact cgctcacgct gcgcggggag
     7321 gtggggcgcg tctgggccga tgcgcgcact accgtgcccg acgatttcgg gttccgcacg
     7381 ggcggcgcgc gcacggtgcg cggctattcc tacctgagcc tgggcgagaa gcgcgacaac
     7441 gccgtggtgg gcgcgcccgc gctggcggtg gccagcatcg agtacgacca ttacttcaac
     7501 taccgctggg gcatgggcgt attcgtcgat gcgggcgacg cggccgcctc gttctcgagc
     7561 ctgcaccctg ccgtgggcta tggcgtgggc gcgcgcgtgc gcacgcccgc cgggccgctg
     7621 ttcctggacg tggcctatgg ccagcgcgac caccagctgc gcctgcactt ttccctgggg
     7681 atcgcgtttt gaagacggtc ctggccctgc tgcgcgccgt gctgcattgg ctgctggtct
     7741 ggctgctgcc gggcctggcc atgctggcgg cgctgtgcct ggcctgcgcg ctgtggctgc
     7801 tcggctcgca agccggcgcg cagctgctgc tgccgggcgc gctcgaactg ctcggcggcc
     7861 acgccgaggc cgtgcagggc agcgtgctgc gcggcctgcg cgtggggcgc ctgcaggctg
     7921 ccttcggcgg caccgaagtg gacgtcgagg gcctggcgct ggaggtcgac tggcgcgcct
     7981 tgctggcgcg ccgggcgcat gtgcggctgc tcgcggccga ccggctgcgc atcgtcctgg
     8041 cgggcgcggc agccggcgcc ggaccggacg atgcccccat ggcgcccatt accttgccgg
     8101 tggcggtggc gctggaccgt ctggaactgg gcagtttcga gctcgtgcgg gacgggcagc
     8161 ccctgccggt atcgctggac ggcctgtcgg ccagcctggc ggcggacgcg gacggcgcgc
     8221 gcctgcgggt cgacggcctg cgggcggggc acgcgcagga acaggcgcag gtccagggca
     8281 gcgtcgaact ggggccgcag ccctggtccg cgcagtcgct gcgcgccctg gcgctggatc
     8341 tgtcgcgcga gggcgggcag gacttgtcgc tgcgcgtcgg caggcaggcc ctggatgcgc
     8401 gcaccgacag gctggccggc acgctgcggg ccagccgctt cgacctcggc agcctgctgg
     8461 ggccgggcct gccgcaggcg ctgcttgatc tgcaggccga tttcgaggcc gatgtcgccg
     8521 gcctgcaaac cctgcggcgc ctgcatgcat cggcgcgcat cggcggggac agccgctgga
     8581 acggccaggc gttgtccggc accctggagg ccggcatccg ggccctggac gcgccggcgg
     8641 atccgcaggc gccgctgccg ggctgggcgc tggacgccgc gcaggccgac ttgcgcctgg
     8701 gcgccagccg catcagcctg cgaggacagg cggatacgca ccaagggcaa ctggcgctgg
     8761 aggcgaatat ccccgccctg gcggcgttct ggccggggct gcccggtggt gcgcggctcc
     8821 aggcgcgggt cgagggcacg gcggccagcc accgcctgac cgcgcaggca agttatgcgt
     8881 ccggccatgc ccaggccggg cggctgggca tggcgccgct gcggctggac gtggccgcgc
     8941 agggcggctg ggggccgggg ccggacggcg cgcccacggg ctggcgcggt acggtgagcc
     9001 ggttgtcggc cgatagtgcg gggttctcgg tagcgctcgg ccggccggca ccgctcgcct
     9061 ggctgccggg cgcgcaggcg ccgcaatggg gcatccaggt cggtgccacg actttgtccc
     9121 tggcttttcc cggcgggcag cgcctggtgc tgtcgcatgc cggttcgcgc ctgggcggcg
     9181 ggcgctggga gacggccggt cggggtgatg gcttggcgct caccacggac atgatgaacc
     9241 agttgcaccg cgccctgggc gcgccggtgc gggccggcgg cgatgccgcc ggccgcggcg
     9301 cggcgcccag ccagcgcgtc gtgctcgatg cctcgtggaa cctgcgcttc gacggcgcgc
     9361 tggccggcca ggcccgcatc gagcgtcgcg cgggcgatct gctcatccct ggcagcccgc
     9421 cgatctcgct gggcttgcgc ggcctgacgc tgtcggtcgc ggcgacgccg accggcccgg
     9481 cgaccagccg cctggatgcg agcctggact tgcgcaccga gaaaatgggc acgctgcagg
     9541 cccgcgccag cgccatgctg gccgccctgg ccctggacgc gcgccagccc gtgcgcacga
     9601 gcctggacgc cgacatcggc gaccttgcct ggctgggatt gttcaccggc gatgcctatg
     9661 ctttcggcgg cgcgctgcgc gtccaggcgc aggcccaggg cacgccgcag ggcaagtggt
     9721 cggcccaggg cagcctgcat ggcaccgggc tgcgcatgct gcggctcgag gacggcgtgc
     9781 agctggtcga cggcacgctc gacgcgcact tggacggcga ccgcttcgtg ctggacgcgc
     9841 tgcgctttcc cgcctcgcgg caggtgatgc cccaggacgc gcgcacgcgc gactgggtcg
     9901 agcacgatcc ggacgcccag ggtggcgatg tcgaggccag cggccactgg gacctggccg
     9961 agtcgcgcgg gcaggtcgac gtgcggctgc atcgctttcc ggccctgcag cgcgccgacc
    10021 gctacgccat gctgagcggc gcgctgcggc tggacgccga gttgccgcgc ctgcgcctga
    10081 cgggcgacct gacggccgac gccggctggg ccagcgtgga catcctgcag ggcgttccca
    10141 cgctggacga cgacgtgcac gtggcgcgtc ccggccagga gagcgcggcg ccggccacgc
    10201 ccctgcagtt cgacatggac atcggcgtga acgccgggcc gcgctttttc ctgaccggca
    10261 tgggcctgga tgcgggcctg cagggcagcc tgcgcatcgt gatgcaggat ggccagttgc
    10321 gcggcaccgg cgaactgcgc acccgcgcgg gcaccttcca ggcctatggc cagaagctgc
    10381 agctgcggcg cggcatcgtc accttccagg ggcggctcga caatccgctg ctcgacatcg
    10441 aggccgtgcg caccgatata cagcaggtgc aggcgggggt gcgggtggcc ggcacggctc
    10501 agcggccgcg catcgacctg atctccacgc ccgacatgag cgacgtggag aagctgtcgt
    10561 ggctggtcat gggccgcgcg cccgacgcca gcgggtccga cacggcgctg ctgctgtcgg
    10621 tgggcgcgtc cctgctgggc ggcgatacgc ctttctataa gcagctgggc ctggacgatg
    10681 tcggcatccg caccggcacg ctgggcagct cgggcagcct gctgcccgac cagacggtgg
    10741 ccaccaacgc cgtcgacagc accagcgcca gcgacctggc cactcagttc atcgtggcca
    10801 gcaagcattt cgccaacggc atcacgctca gcgtggagca ggccatgtcg ggcaccggca
    10861 cggtgggccg ggccagctac cgcctggcgc gcggactgtc gctggacctg aaggcgggcg
    10921 aggtcaacgg cctggacctg gtgtaccggt ggtttctggg cgaatgagta aaatacgcgc
    10981 cttgtacaga tcgcaactcg gaattcgggg cacccatcca tgagcatcaa gagcgaccgc
    11041 tggatacgcc aggccgccca ggccggcatg atcgagccct tcgaggcggg ccaggtgcgc
    11101 aacgtcaacg gcgagcgcat cgtcagctac ggcaccagca gctacggcta cgacgtgcgc
    11161 tgcgccgccg aattcaagat cttcaccaat atcaactcca ccatcgtcga ccccaagaat
    11221 ttcgacgaga agtccttcgt ggacttcaag ggcgacgtct gcatcatccc gcccaactcg
    11281 ttcgcgctgg cccgcacggt ggagtacttc cgcatcccgc gcagcgtgct caccatctgc
    11341 ctgggcaaga gcacctacgc gcgctgcggc atcatcgtca acgtgacgcc gctcgagccc
    11401 gagtgggaag gccacgtcac gctcgagttc tccaacacca ccccgctgcc ggccaagatc
    11461 tacgccggcg agggctgcgc ccagttcctg ttcctggaaa gcgacgaggt ctgcgagacc
    11521 tcctacgccg accgcggcgg caagtaccag ggccagcgcg gcgtgaccct gccgcgcacc
    11581 tgatatggcg ggcgctagct ccgcgggata ggccgcgcca tcaggatctc gtcgtacagc
    11641 gcgcccgcca cgcaaagcgc ctcgggttcc cggccgtatt cccgaaagcc cgcgcgttca
    11701 tataacgcga tcgccggcgc gttgccggca ttgactgaca attgcacttg gcgcaagcca
    11761 ggcagcgcgg cggcgctggc cagggcggct tccagcagcc tgccgcccag gcccagcccg
    11821 cgctgcccgg gttcgatgta catgccccag atataggcct tgtggcgcat cttggaccgg
    11881 ggttgccgcg tcatgccgac catgccggcc aggtggccgc cgacgaaggc gccgaatagc
    11941 gcgctttctt tgccagaggt ccattgccgg aagccgtcca ggtccatcgc ctgctcttct
    12001 gccaggctcg acccgaacgc gtgcggcgcc tcggatagcg catcgaggcg caggtcccgc
    12061 agggcggggg catcatcggg ggcgagacgg cgtatttgga cgggcatggc gctggcgggc
    12121 ggggcggccg gttggtatgc cggtcagtat ggcggcggca atggcgcggg gcaaggtttc
    12181 ccgctgccaa accgtgtcaa cagcctgtcc cggactcgca gtacactcgc gtccgttatt
    12241 ggcccgaggc cccgccgtcg gtgtgtacag gccccaccct cctttggagc gcctgcatga
    12301 aatttcgttt tcccatcttc atcatcgacg aagattaccg gtccgagaac gcctcgggcc
    12361 tgggcatccg ggccctggcc tcggccatcc aggccgaggg catggaagtc atcggcgtca
    12421 ccagctacgg cgacctgagt tcctttgccc agcaacagag ccgcgcgagc gccttcatcc
    12481 tgtcgatcga cgacgaggaa ttcgacgaca gctcgcccga agacgtggcc agcgccatca
    12541 agaacctgcg ctcgttcatc ggcgaactgc gctttcgcaa cgccgacatt cccatctacc
    12601 tgtatggcga gacgcgcacc tccgagcaca tccccaacga catcctgcgc gagctgcacg
    12661 gcttcatcca catgttcgag gacacccccg aattcgtggg gcggcacatc atccgcgagg
    12721 ccaagagcta tgtcgacggc ctggcgccgc cgtttttcaa ggagctggtc gattacgcgc
    12781 aggacggctc gtactcgtgg cactgtcccg gccactcggg cggggtggcc ttcctgaaga
    12841 gcccggtggg ccagatgttc caccagttct tcggcgagaa catgctgcgc gccgacgtct
    12901 gcaacgccgt ggacgaactg ggccagctgc tggaccacac cggccccatc gccgagtccg
    12961 agctgaacgc ggcgcgcatc ttccacgccg accactgctt cttcgtgacc aacggcacct
    13021 ccacgtccaa caaggtggtg tggcatgcca acgtggccgc gggcgacgtg gtggtggtcg
    13081 accgcaactg ccacaagtcc atcctgcacg ccatcacgat gacgggcgcc atcccggtgt
    13141 tcctgcgccc cacgcgcaac cacctgggca tcatcggccc gattccgctg gaagagttcg
    13201 accccgagaa catccgcaag aagatcgagg ccaacccttt cgcccgcgag gcgctcgaca
    13261 agcggccgcg cattctcacg ctgacgcaga gcacctacga cggcgtgatc tacaacgtgg
    13321 aaatgatcaa ggaaaagctg ggcacctaca tcgacacgct gcacttcgat gaagcctggc
    13381 tgccgcacgc gtcgttccac gagtactacc aggacatgca tgccatcggc cagaaccgtc
    13441 cgcgcagcaa ggaggccatg gtgttcgcca cgcactccac gcacaagctg ctggccggca
    13501 tttcgcaggc ctcgcagatc atcgtgcagg agtccgagac ccgcaagctc gaccgcaata
    13561 tcttcaacga ggcgtatctg atgcacacct cgacgtcgcc gcagtacgcc atcattgcct
    13621 cgtgcgatgt ggccgcggcc atgatggagc cgccgggcgg caccgcgctg gtcgaggaaa
    13681 gcatcaagga ggccctggat ttccgccgcg ccatgcgcaa ggtggagtcc gagttcggca
    13741 agaacgactg gtggttcaag gtgtggggcc cggaccgcct gccggcgtcg ggcatcggca
    13801 gccgcgatga ctggatcctg gaatccaatg cgcactggca cggcttcggc gacctggccg
    13861 agggcttcaa catgctcgac ccgatcaagg ccacggtggt gacgccgggc ctggacatgt
    13921 cgggcagctt cggcgagacg ggcattccgg ccgcgctggt gtccaagtac ctgaccgagc
    13981 acggcgtggt ggtcgagaag accggcctgt attcgttctt catcctgttc accatcggca
    14041 tcaccaaggg ccgctggaac acgctgctca ccgccctgca gcagttcaag gacgactacg
    14101 accgcaatca tccgctgtgg cgcatcctgc ccgagttctg ccgcgaccat cgcggctacg
    14161 agcgcctggg cctgcgcgac ctctgccagc agatccacga ggcctaccgc gcgcacgacg
    14221 tagcccgtct gaccaccgag atgtacctga gcgacatggt gccggcgtta aagccgtccg
    14281 acgcctttgc ccgcatggcg caccgcgagg tcgagcgcgt gacgatcgac gagctggaag
    14341 gccgcgtcac gggcgtgctg ctcacgcctt atccgccggg cattccgctc ctgatcccgg
    14401 gcgagcgctt caaccgcacc atcgtgcagt acctgcagtt cgcccgcgag ttcaatgcgc
    14461 gctttcctgg gttcgagacc gacatccacg gtttggctga ggacgttggt ccggacggcg
    14521 agacgcgcta ctacgtggat tgcctcatcg aggattgagc ggttcttgcg ccgcatcgga
    14581 gccgagggcg caaagcgctc cggcttcgat gaattgggct cgggcgcatg caggcgccct
    14641 cggccccctg cgggggctgc cccaattcat aggcgcctgc gcgctttgcg ccctcggctc
    14701 cgatgcttag aaccgttcta tcgagaacgg cccgaggatg cgttgggcat cgggctggcc
    14761 ggccatggcg ccggcgatca gcttgcccgt gttggccgat ccggtcaggc ccaggtggcc
    14821 gtggccaaag gcgcaccaca ggccgttgcg gccgggcacg gggcccagca ccggcatgga
    14881 gtcgggcagg caggggcggt gtcccatcca ggtggcggcg ccgcgggtgt ccaccgtcgg
    14941 caggccggcc cgggcgtgtt cgcccagcag cagggcgcgc tgcatgctgg ggggatgcgt
    15001 caggctgccg aactccaccg tgccggccat gcgcaggccg ttctccatgg gggtgatgaa
    15061 caccttgcgg t
//
LOCUS       DASWPO010000163        10779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1823980, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000163 DASWPO010000000
VERSION     DASWPO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823980"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VGC69_08965"
     CDS             complement(<1..570)
                     /locus_tag="VGC69_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0295445.1"
                     /translation="MDSQRRADVIVVGLGAFGSATVYQLARRGVSVIGIDQFQPPHDR
                     GSSHGETRITRLAVGEGEAYVPLIRRSNEIWQALEAETGETLVHRVGGLIVGPRDGTI
                     SHHGKSDFVRRTIETAERFGIEHEVFDAQEFAYRYPQFMPRGDELVYAEPASGVLVPE
                     ACVQAQLDLARAHGAAIRFNEKVLSISETA"
     gene            721..1701
                     /locus_tag="VGC69_08970"
     CDS             721..1701
                     /locus_tag="VGC69_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295446.1"
                     /translation="MKRPSLIETHALRVFVVAAQERNMSTAAARLGLSQSAVSQTIRN
                     LENQVGAILVNRQDRPISLTPAGSEMAHRGALLLDGMIDLGNAVADLAKGVNPNIHLG
                     IVDSFAATCGGRLARALAARTTRLAVRTGLTPSLEQQLLKRELDLIISSEAGSDTPAI
                     KRYELFRESFLAITPKAKHASQACVQDLRELAAGLPLIRYNLDSHLGMQLEEVVQRHQ
                     IKSPPLLEFDTADTLTSMVSEGLGWALTTPLCALQAEHYAERIDYAFIGDLQGVRSLY
                     LAARSDQHEGFVEDALEICRELIRTDLIPRLRQIHPRLADCVTPSAQAPA"
     gene            complement(1673..3058)
                     /locus_tag="VGC69_08975"
     CDS             complement(1673..3058)
                     /locus_tag="VGC69_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0295447.1"
                     /translation="MASLTLAIAQYAVSRMTVPAAIAAIAKSGFIDCAACIVAGRNDG
                     AVLALRRFIRDQGRGGAARAHAWTDGTQLAAADAAMLDATAAHALDYDDVALAGHPSA
                     VLVPAILAAAQSVPQCNGMDVLRAYVIGYEVWAELYRRDQDALHQKGWHPSSTLGLVA
                     ATAAVISVSGLRQDIAQHALGIACSRAAGVTANFGSATKPLHIGYAASEAMTAVALAR
                     CGMTSAADAIEHGSGLLNALSPAGRVDLSGEVGLSDGSAYVARYGLSIKRYPVCYALH
                     RVLDGILALRAQPGFDTRQIEAIELCVGDTQANMLRVEAPQTTLEAKFALKFCVAVAA
                     VRGRLGMAELDPGLMRHPDVAHLLPRIRVTIDTAKCDVYPWFSPADTVTVRLADARVL
                     SSGPIRFAKGNAHNPVDAAALKEKYEDCLAGQDTLAAAPLHACLATLEAVPDIASALA
                     ACIKPAPAPRA"
     gene            complement(3088..4044)
                     /locus_tag="VGC69_08980"
     CDS             complement(3088..4044)
                     /locus_tag="VGC69_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295448.1"
                     /translation="MRKLLFWMWGCLACYQSVASAAYPEHAITIVVPFAAGSATDNIV
                     RALASKLSPKLNVPIIVEDRAGANGIIGTSHGAKAEHDGYTLTAITGTTIAQNPWMFK
                     DMSYDPLKDFQAVGRLGGFTLAIVVKADSPYHSYSELLSDIRQGKNNVSYATSYGMQT
                     VCGEMVGRDAGGKVLSVPYKSSPQSMVDLMGGMVTFVCADLATSLSGLNGKTIKALTV
                     LMPEGSRYLPDVVPVQKSWPKFPAMQSWVGIAAPTGIPQDRLKILSDAVYSVALDPDF
                     AKSLAAVGFASTPMDAQKFTAYMHDEYSRWKMLIQQAGIVPQ"
     gene            4334..5125
                     /locus_tag="VGC69_08985"
     CDS             4334..5125
                     /locus_tag="VGC69_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0295449.1"
                     /translation="MPTLSVDGARIYYELAGEGPAVVVTAGQGTGPQARQALIDGLAR
                     RHTVLTYDPRGTGRSDPVPQGHDIETLAQDVLALMDAVKFEKARVIGLSTGTGKATVL
                     AAQHPDRVERLILAAPWTHGDPEFFILQNLRKAAARTMPPEHYAHFNALLLYCPAYRR
                     EHAEQFAEMARRAIGLQQDAEGTAARLDAILRFDARPLYGRIACPTLVFGARDDLVMP
                     LWFAQDAARAIPGAPDHHRRRRPHVLRIAHRCISARRPALSGLTD"
     gene            5134..6114
                     /locus_tag="VGC69_08990"
     CDS             5134..6114
                     /locus_tag="VGC69_08990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0295450.1"
                     /translation="MPEANLHTSSIVIDGLVVSRWSREVFEDMRRGGLTAANCTCSVW
                     EGTRDTMLYMARWNRYFDEYGDLIMPVRSAGDIRKAKDLGKVGILLGWQNTAALEDRI
                     DFLPLFKDMGLRVIQLTYNTQNLVGAGCWEENDSGLSGFGREVVEEMNRLRLLVDLSH
                     VGQKTASDAIAHSKRGCAYTHVAPYGLFNHARNKTDEQLREVVERGGFVGVATYPPFM
                     KTGAETNLDDCIDLFEYMLNVCGESNIGIGTDFTQGYGDDFFDWLRQDKGYARRANPG
                     RGRAPTVKGMETLASYPKLTGAMQRRGWHETRIRGVLGENWLRFLEESIG"
     gene            complement(6179..6814)
                     /locus_tag="VGC69_08995"
     CDS             complement(6179..6814)
                     /locus_tag="VGC69_08995"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0295451.1"
                     /translation="MKTAASLGLEPRKTPRQARSEATVAAIYEATIQVLLAEGVRRLT
                     TTRVAERAGASVGTLYQYFPNKQALLHSVLALHLDGVADAVEAACRGPLADGLEARVA
                     TIVQAYMDAKLSRPDVSRVLYQIYAELNATELVGRVGGRIRAAVTDALAGAGEIRAED
                     LPMAVFMFQSALAGIARSFLEQGAPRRFRSGLREQAALLCMAYLRARTPAP"
     gene            6895..7626
                     /locus_tag="VGC69_09000"
     CDS             6895..7626
                     /locus_tag="VGC69_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0295452.1"
                     /translation="MATLPRIALITGANKGIGFETARQIGHAGLRVLMGARAPALGEA
                     AAAQLRSAGIDARFIHLDLGDPATMAAAAAAIEAAYGRLDVLVNNAGITDRADAPPSR
                     ASLDAVRRIFDTNVFGSLAITQAMLPLLRKSSAARIVNVSSGLGSLAFNGDPAWEFAS
                     VKLFGYNASKAAVNMLTVQLAAELPPAGIKVNAADPGFTATDLNGHRGHQTVEQGAEV
                     VIRLALLPDDGPTGGFFCTQGSNPW"
     gene            complement(7649..9157)
                     /locus_tag="VGC69_09005"
     CDS             complement(7649..9157)
                     /locus_tag="VGC69_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY0295453.1"
                     /translation="MTLAVLASRALLGLEAPAVRVETHVGSGLPAFHIVGLPEAEVRE
                     SRERVRAALVNSGYEFPAGRITVNLSPADLPKASARFDLPIALGVLLASGQIAPPAGQ
                     PPEALADIMLAAELSLTGALVPVASALPLALGLARERPGGAMVLPPGSAGIAAWVPGL
                     RVYVARTLAEAAQHLAGTVQLPLAEPAARPQAEAASPCLSDVKGQAGARRALEICAAG
                     GHSLLMSGPPGSGKSMLAQRLPGLLPPLSPVQALEVAALACLAGAGPQAGISERPPLR
                     APHHTASAAALVGGGGRPRPGEISLAHHGVLFLDELPEFRRAALEALREPLETGRVAI
                     SRALRQVEFPARFQLVAAMNPCPCGWRGHPRKACRCTPEQVERYAGRVSGPLLDRIDL
                     HVSVPPADLGWMDGPPGEPSSAVRERVARCRARQHARQGRPNAALQGAELERHCPLSP
                     PARELLQQVMHRLSGSTRGLHRALRVARTLADLDASDIIETRHVAEAAQYRR"
     gene            9504..>10779
                     /locus_tag="VGC69_09010"
     CDS             9504..>10779
                     /locus_tag="VGC69_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295454.1"
                     /translation="MLLAGQLLPMIDFSIVNVALDAMAASLHATEVQLELIVAVYGVT
                     FAVCLAMGGRLGDNFGRRRLFGWGVALFTLASLLCGVATSVWMLLGARILQGAAAALA
                     MPQILATIHVGLRGREHSRALGLYGAIGGLAFVVGQVLGGLLITLDIGGLGWRSVFLI
                     NLPIGLAVLACTRRLVPETRSQHPARVDGPGTLLLALVILCLLLPMSLGPVLHWPWQC
                     GVALGAVPVLLYAVWRVELRQERRQAFPLLPPTLLRLSSMRFGIWIALLFFSGWSGFL
                     FCMALTFQAGAGMSPLQSGDAFIAMGSAYFIGSLCTSRAAARFEQTTVLLFGCLMQMA
                     GLLGTMYTLWRVWPAPDALNLIPATILIGFGQAFIVGSFFRIGLSEVRPEQAGAGSAT
                     LSTVQQSALGLGPALLGAVLTHFLNTREHDYLT"
BASE COUNT         1754 a         3728 c         3537 g         1760 t
ORIGIN      
        1 ggcggtctcc gaaatcgaca gcactttctc attgaagcgg atcgcggcgc catgcgcccg
       61 ggccaaatcc agttgcgcct gcacgcaggc ttcgggcacg agcacgcccg aggccggttc
      121 agcatagacc agctcgtcgc cgcgcggcat gaactgcgga tagcggtagg cgaactcttg
      181 cgcatcgaac acctcgtgtt cgatgccgaa gcgctcggcc gtctcgatcg tgcggcgcac
      241 gaaatcggat ttgccgtgat ggctgatcgt gccgtcccgc ggcccgacga tcaggccgcc
      301 gacccggtgc accagcgtct cgccggtttc cgcttcgagc gcttgccaga tttcattcga
      361 ccgccggatg agcggcacat aggcctcgcc ctcgcccacc gccagccggg tgatccgggt
      421 ctcgccgtgg ctggacccgc ggtcgtgggg cggctggaat tgatcgatgc cgataacgct
      481 gacgccgcgg cgcgccagtt gatagaccgt cgcgctgccg aatgctccca ggcccaccac
      541 gatcacgtcc gccctgcgtt gactatccat ctttccaagc ctgcttttat tgatatggct
      601 tcatactagg tgcggcatgg aaggatcgca ataaaccggc atgcaagata agcggttctg
      661 atagtatgaa tcagttgggt gcgtattgat tcttccgtgc gctggcagcg catctcgctc
      721 atgaaacgac cttcacttat cgaaacgcat gcgctgcggg ttttcgtcgt cgccgcgcaa
      781 gaacgaaaca tgtccaccgc cgcggcgcgg ctgggcctga gccagtcggc cgtctcgcag
      841 accatacgca atctcgaaaa ccaggtcggc gccattctgg tgaaccgcca ggatcgcccc
      901 atttcgctga cccccgcggg cagcgaaatg gctcatcgcg gcgccctgct gctcgacggc
      961 atgatcgacc tgggcaatgc ggtcgcggac ctggccaaag gcgtcaaccc caatattcac
     1021 ctgggcatcg tggactcctt cgccgcgacg tgcggcggac gcctggcgcg cgccctggcc
     1081 gcccgcacca cgcggctggc cgtgcgcacc gggctcacgc cctcgctgga acagcagctc
     1141 ttgaagcgcg agctcgacct catcatctct tccgaagccg gcagcgacac gcccgcgatc
     1201 aagcgctacg aactgttcag ggaatcgttc ctggccatca cgcccaaggc caagcacgcg
     1261 tcgcaggcct gcgtgcaaga cctgcgcgag ctggccgcgg ggctgccgct gatccggtac
     1321 aacctcgatt cgcacctggg catgcaattg gaagaagtcg tccagcggca ccagatcaag
     1381 tcgcctccct tgctggagtt cgacacggcc gacacgctga cgtcgatggt ttccgagggc
     1441 ctgggctggg ccctcaccac gccgctgtgc gccctgcagg ccgagcatta cgcggaacgc
     1501 atcgactatg ccttcatcgg cgatctgcaa ggcgtgcggt cgctgtacct ggccgcccgg
     1561 tcggaccagc acgagggctt cgtcgaagac gccctggaga tttgccgcga actgatccgc
     1621 acggatctca tcccccggct gcggcagatc cacccccggc tcgcggattg cgtcacgccc
     1681 tcggcgcagg cgccggcttg atgcacgcgg ccagtgccga ggcaatgtcc ggcacggctt
     1741 cgagcgtcgc aagacacgca tggagcggcg ccgccgcgag cgtgtcctgg cccgccaggc
     1801 aatcttcgta tttttccttc agcgccgccg cgtcgacggg attgtgcgca ttgcccttcg
     1861 cgaaccggat cggcccgctg ctcagcacgc gcgcgtcggc cagccgcacg gtgacggtat
     1921 ccgccggaga gaaccacggg tagacgtcgc acttcgccgt gtcgatcgtg acgcggatgc
     1981 gtggcagcag gtgcgccacg tcggggtggc gcatcagccc gggatcgagc tcggccatgc
     2041 ccagcctgcc ccgcacggcc gccaccgcga cgcagaactt gagggcgaac ttggcctcga
     2101 gcgtggtctg cggcgcttcg acgcgcagca tattggcctg ggtatcgccg acgcacagct
     2161 cgattgcctc gatctgccgc gtgtcgaaac cgggctgcgc gcgcagcgcg agtatgccgt
     2221 ccaacacgcg atgcagcgca tagcagaccg gatagcgctt gatggacagg ccgtatcggg
     2281 ccacataggc cgagccgtcg ctcaggccga cctcgccgga aagatcaacc cggcccgccg
     2341 gcgaaagcgc gttcagcagg cccgagccat gctcgatcgc atccgcggcc gaggtcatgc
     2401 cgcagcgggc cagggcgacc gcggtcatgg cctcggaggc ggcatagccg atatgcaagg
     2461 gcttggtcgc cgaaccgaag ttggccgtga cgcccgcggc ccggctgcag gcgatgccca
     2521 gcgcgtgctg cgcgatatcc tgccgcaggc cgctcacgct gatgacggcg gccgtcgcgg
     2581 cgaccagccc caacgtggaa gaaggatgcc agcccttctg gtgcaaggcg tcctggtctc
     2641 ttcggtagag ctcggcccag acttcatagc cgatgacata ggctcgcaat acgtccatgc
     2701 cgttgcattg cggcacggat tgcgccgcgg cgagtatggc cggcaccagc acggcgctgg
     2761 gatgccccgc cagcgccacg tcgtcgtagt ccagcgcgtg cgccgccgtc gcatcgagca
     2821 tggcggcatc ggccgcggcc aactgcgtgc cgtctgtcca ggcatgcgct cgcgccgcgc
     2881 cgccgcgacc ctgatcccgg atgaaccgcc gcagcgccag gaccgcgcca tcgttgcggc
     2941 cggcgacgat gcaggccgcg cagtcgatga agccggactt ggcgatagcg gcgatcgccg
     3001 cggggaccgt catgcggctt accgcgtatt gcgcaatggc gagtgtcaga gaggccatgg
     3061 ttcaccttgc cggttcggcg ccgccgctca ctgcggcacg atgcccgcct gctggatcag
     3121 cattttccag cgggagtatt catcatgcat gtacgccgtg aatttttgcg catccatcgg
     3181 ggtggatgcg aagccgacgg cggcaaggga ttttgcaaaa tcggggtcca gcgcgacaga
     3241 gtaaaccgcg tcgctcagga tcttgagcct gtcctgggga atgccggtgg gcgcggcgat
     3301 gcccacccag gactgcatgg cggggaactt gggccaggac ttctgcacgg gaacgacgtc
     3361 cggcagatag cgggatccct cgggcatcag cacggtcaag gccttgatcg tcttgccgtt
     3421 caaaccgctc aggctggtcg ccagatcggc gcaaacgaag gtcaccattc cgcccatcag
     3481 atccaccatc gactgcgggc tcgatttgta gggcacgctc agcaccttgc cgcccgcatc
     3541 gcggccgacc atttccccgc acaccgtctg catgccgtag gacgtggcat acgagacatt
     3601 gttcttgcct tgccggatat cgctcaacag ctcgctatag ctgtgatagg ggctgtcggc
     3661 cttgacgacg atggccaggg tgaaaccgcc cagccgtccc accgcctgga aatccttgag
     3721 cgggtcgtag ctcatgtcct tgaacatcca gggattctgg gcgatggtcg tgccggtgat
     3781 cgcggtcagc gtatagccgt cgtgctccgc tttcgcgcca tggctcgtgc cgatgatgcc
     3841 attggcgccc gccctgtcct ccacgatgat cggcacgttc agcttgggcg aaagcttgga
     3901 ggcgagcgcc ctgacgatat tgtcggtggc gctgcccgcc gcaaaaggca cgacgatagt
     3961 gatggcatgc tcgggataag ccgcgctcgc aacactttga taacacgcca ggcaacccca
     4021 catccagaac agcagtttcc tcatgatttc cccttgcgaa atcgctttat ctaacctggg
     4081 ctctctctgc gtaggccctg ttggtggcac tatcctgcgg catcgctccc gtgaagtcca
     4141 tgcgggatac gggcagcatc agcgcaattg atcgtgaggt gcacgccgct taatactggg
     4201 gattccccat gcgtacaggg cgcggcctct tgcactaagg tagccacagc cagcacgccc
     4261 tcccgggcgt tgctggtatt tacccgggat cggcacttgc gaaccgggca ggcacggcaa
     4321 ccgaggagcg ggaatgccta cactcagcgt cgacggcgcg cggatttact acgagctcgc
     4381 gggcgaaggg cccgcggtgg tggtcacggc aggccagggc accggcccac aagcccgcca
     4441 ggcgctgata gacggcctgg cgcgccgcca caccgtgctc acctacgacc cgcgcggaac
     4501 cggccgcagc gaccccgtcc cccaggggca cgacatcgag acgctggcgc aggacgtcct
     4561 ggcattgatg gacgccgtga aattcgagaa ggcgcgcgtc atcggattgt ccaccggcac
     4621 gggcaaggcc acggtgctcg ccgcgcaaca cccggaccgg gtcgagcgcc tgattctcgc
     4681 cgcgccgtgg acgcacggcg acccggaatt cttcatcctg cagaacctgc gcaaagccgc
     4741 cgcccggacg atgccgccag aacactacgc gcacttcaat gcgctgctgc tgtactgccc
     4801 ggcctaccgc cgcgagcatg ccgaacaatt cgcggagatg gcgcgccggg ccatcggcct
     4861 gcagcaggac gccgaaggca ccgcggcccg cctggacgcc atcctgcgct tcgacgcccg
     4921 ccccctgtat ggccggattg cctgccccac gctggtcttc ggcgcgcgcg acgatctggt
     4981 catgcccctg tggttcgcgc aggatgccgc gcgggcgatt cccggcgcgc ctgatcacca
     5041 tcgaagaagg cggccacatg ttcttcgaat cgcgcaccga tgcatttctg caagacgccc
     5101 tgccctttct gggctgaccg actaggaacc gacatgcctg aagcaaacct gcacacatcc
     5161 agcatcgtca tcgacggact cgtggtgtcc cgctggagcc gggaggtctt cgaagacatg
     5221 cggcgaggcg gcctgaccgc cgccaattgc acctgctcgg tctgggaagg cacgcgcgac
     5281 accatgctct acatggcccg ctggaaccgg tacttcgacg agtacggcga cttgatcatg
     5341 ccggtgcgca gcgccggcga catccgcaaa gccaaagacc tgggcaaggt cggcattctg
     5401 ctcggctggc agaacaccgc cgccctcgaa gaccgtatcg acttcctgcc gctcttcaag
     5461 gacatgggcc tgcgcgtcat ccagctgacg tacaacaccc agaacctggt gggcgcgggt
     5521 tgctgggaag agaacgacag cgggctgtcg gggttcggcc gcgaggtcgt cgaggaaatg
     5581 aaccgcctgc gcctgctcgt cgacctctcg cacgtggggc agaaaacggc atccgacgcg
     5641 atcgcgcact ccaagcgggg ctgcgcctac acgcacgtcg ccccttatgg actgttcaac
     5701 cacgcgcgca acaagacaga cgagcaactg cgcgaagtcg tggagcgggg cggcttcgtg
     5761 ggcgtcgcca cctatccgcc cttcatgaaa accggtgcgg agaccaacct ggacgattgc
     5821 atcgacctct tcgaatacat gctgaacgtg tgcggggaaa gcaatatcgg catcggcacc
     5881 gacttcacgc aaggctacgg cgacgacttt ttcgattggc tcaggcaaga caagggctat
     5941 gcgcgccgcg cgaacccggg gcgcggcagg gcgcctacgg tcaaaggcat ggaaaccctc
     6001 gcaagctacc cgaagctgac cggcgccatg cagcgacgcg gctggcacga aacgcgaata
     6061 cgcggcgtgc tgggagaaaa ctggctgcgc tttctggaag aaagcatcgg ctgatgtcca
     6121 acgggcgccg gccgcggcat tcgggttgag agcgtagcgg cccgcacgtt cgcgcccgtc
     6181 aaggggccgg cgttcgggcg cgcagatagg ccatgcagag cagggccgcc tgttcgcgca
     6241 agccgctcct gaaacggcgc ggcgcgccct gctcgagaaa cgaccgggcg atgccggcca
     6301 gcgcgctctg gaacatgaag accgccatgg gcaggtcttc ggcgcggatc tcgccggcgc
     6361 cggccagtgc gtccgttacc gcggcccgta tccggccgcc cacccggccc accagttccg
     6421 tcgcgttgag ttccgcgtag atctggtaga gcacccggga cacgtcgggg cgggacagct
     6481 tcgcgtccat ataggcctgg acgatcgtcg ccacccttgc ctccaggccg tccgccaacg
     6541 ggccgcggca cgcggcctcg accgcatccg ccaccccatc caggtgcagc gccaggaccg
     6601 aatgcagcaa tgcctgcttg ttcggaaagt actggtagag cgtccccacc gaggcgcccg
     6661 cgcgctcggc gacccgcgtg gtcgtcaggc gccgcacgcc ttcagccagc aaaacctgaa
     6721 tggttgcctc gtagatggcg gccaccgtgg cctcggaacg ggcctggcgc ggcgtctttc
     6781 ttggctcgag gccgagcgaa gcggcagtct tcatatgcga attcaaaata tgaataatcc
     6841 ttcataaact ggagtctata cctgaaccac gcccaagcac ctggaaaacc cgccatggcc
     6901 accctgcccc gcattgcctt gatcaccggc gccaacaaag gcatcggatt cgaaaccgcc
     6961 cggcaaatcg gccatgccgg cttgcgcgtg ctgatgggcg cccgcgcgcc agcgcttggc
     7021 gaggccgcgg ccgcccaact gcgcagcgca ggcatcgatg cccgcttcat ccacctggac
     7081 ctgggcgatc cggcgaccat ggccgctgcg gcggcggcca tcgaggccgc gtacggccgg
     7141 ctggacgtgc tggtcaacaa tgccggcatc accgaccgcg ccgacgcccc gccgagccgg
     7201 gccagcctgg atgcagtgcg gcgcatcttc gacacgaatg ttttcggctc gctcgccatc
     7261 acccaggcca tgctgccctt gctgcgcaag tcgtccgcgg cgcggatcgt caatgtatcg
     7321 agcggcctgg gttcgcttgc cttcaatggc gaccccgcct gggaattcgc gagcgtcaaa
     7381 ctgtttggct acaacgcgtc caaggccgcg gtaaacatgc tgacggtgca attggccgcc
     7441 gagctgccgc cagcgggcat caaggtcaat gcggcggacc cgggcttcac ggcgaccgac
     7501 ctgaacggcc accgcggcca tcaaacagtg gaacagggcg ccgaagtcgt gatccgcctg
     7561 gccctgctgc cggacgacgg cccaacgggc gggttcttct gcacgcaagg atccaacccc
     7621 tggtaggcgc cgcccggcgg cctgcccttc agcggcggta ttgcgccgct tccgccacat
     7681 gccgggtttc gatgatgtcc gaggcgtcca ggtcggccag cgtgcgtgcc acccgcagcg
     7741 cgcgatgcag cccgcgcgtc gaaccgctca accggtgcat cacctgctgc aacagttcgc
     7801 gcgccggcgg cgatagcggg caatggcgtt cgagctcggc gccttgcagg gcggcgttgg
     7861 gccggccctg ccgggcatgc tggcgcgccc ggcagcgcgc cacccgctcg cgcaccgcgg
     7921 acgacggctc gcccggcggg ccgtccatcc agcccaggtc cgccggcggc accgaaacat
     7981 gcagatcgat gcggtccagc aaggggccgg acaccctgcc ggcataacgc tccacctgtt
     8041 ccggcgtgca gcggcaggct ttccggggat ggccgcgcca gccgcagggg cagggattca
     8101 tggcggcgac caattggaaa cgggctggaa actccacctg gcgcagggcg cgagagatcg
     8161 ccacccgccc ggtttccaac ggctcgcgca aggcttccag ggccgcgcgc cggaactcgg
     8221 gcagctcatc caggaacagc acgccgtgat gggccaggct gatctcgccc ggtcgcggcc
     8281 ggcccccgcc gcccaccagg gcggccgccg acgccgtatg gtgcggtgcg cgcaggggcg
     8341 gccgctcgga aatgccggcc tgcggccccg cgccggccag gcaggccaag gccgcgacct
     8401 ccagggcctg caccggcgac aagggcggca gcaggcccgg cagccgctgt gccagcatgc
     8461 tcttgcccga ccccggcggg ccgctcatca gcaagctgtg gccgcccgca gcgcagatct
     8521 ccaaggcgcg gcgggcaccc gcctgccctt tcacgtcgga caagcaaggc gatgcggctt
     8581 cggcctgcgg acgcgctgcc ggctcggcca gcggcaattg caccgtgccc gccagatgct
     8641 gcgcagcctc ggccagggtg cgcgccacat agacgcgcag gccgggtacc caggcggcaa
     8701 tgccggcgct gccgggcggc agcaccatcg ccccgccggg ccgttcgcgc gccaggccca
     8761 gcgccaacgg caaggccgag gccaccggca ccagcgcgcc ggtcagcgac agctcggcgg
     8821 ccagcatgat gtcggccagc gcctcgggcg gctgcccggc cggcggcgcg atctgccccg
     8881 aggccagcag cacgcccagt gcgatgggca ggtcgaagcg cgccgaggcc ttgggcaggt
     8941 cggcgggaga aagattgacg gtgatgcggc ccgcgggaaa ctcgtagccg ctattgacca
     9001 gcgcggcgcg cacgcgctcg cggctctcgc gcacctcggc ttcgggcagg ccgacgatat
     9061 ggaaggccgg cagcccgctg ccgacgtggg tttccacccg cacggcgggc gcctccaaac
     9121 ccagcaaggc acggctggct aatacggcta gagtcatgca tgcctcgctc ggctgacgga
     9181 acgaggagta tgcgcgaggc aaagcgccgc ttggcggccg gtgcgacatt catgtcacgc
     9241 agaacccgcc gccttgcgta ccccggatca ccccgccgca cctacgggta ttccccagcg
     9301 gcagccgggt ccatgccata ctctgtgccc aagtgcggtg gtaatcgatc ctcatcccta
     9361 gggtgccggc gccgttcacg cgtcatgcgt cgcccagtcc ttgccccttg cggcacaccg
     9421 cgtcgttttc ctttctcctt ttcccctctc tgtccatgac ggattccgct tctcttgtcc
     9481 ggctcggtcg agccggactg tatttgctgt tggccggcca attgctgccg atgatcgatt
     9541 tttccatcgt caatgtggcg ctggacgcga tggccgcttc gctgcacgcc accgaggtgc
     9601 agctcgaact gatcgtggcc gtctacggcg tgaccttcgc ggtatgcctg gccatgggcg
     9661 gacggctggg cgacaacttc gggcgccggc gcctgttcgg ctggggcgtg gcgctgttca
     9721 cgctggcttc gctgttgtgc ggggtcgcga cgtcggtatg gatgctgctg ggcgcccgca
     9781 tcctgcaagg ggccgcggcg gccctggcga tgccgcagat cctggccacc atccacgtcg
     9841 gcctgcgcgg ccgcgagcac tcgcgggcgc tgggcctgta cggcgcgatc ggcggcctgg
     9901 ccttcgtggt ggggcaggtg ctgggcgggc tgctgatcac cctggacatc ggcggcctgg
     9961 gctggcgcag cgtgttcctc atcaatctgc ccatcggcct ggcggtgctg gcctgcacgc
    10021 gccggttggt gcccgagacg cgctcgcagc acccggctcg cgtcgatgga ccgggtacgc
    10081 tgctgctggc gctggtcatc ctgtgcctct tgctgcccat gtcgctggga cccgtgctgc
    10141 attggccctg gcagtgcggc gtggcgctgg gcgcggtgcc ggtgctgctc tatgccgtgt
    10201 ggcgcgtgga actgcgccag gagcggcgcc aggccttccc gctgttgccg cccacgctgc
    10261 tgcgcctgtc cagcatgcgt ttcggcatct ggatcgcgct gctgttcttc tcgggctgga
    10321 gcggcttcct gttctgcatg gccctgacct tccaggccgg cgccggcatg tcgcccttgc
    10381 agtcgggcga tgccttcatc gccatgggtt cggcgtattt catcggatcg ctgtgcacca
    10441 gccgggccgc ggcgcgcttc gagcagacga cggtgctgct gttcggctgc ctgatgcaga
    10501 tggccggcct gctgggcacg atgtacacgc tgtggcgcgt ctggcccgcg ccggacgcgc
    10561 tgaacctgat acccgcgacc atactgatcg ggttcgggca ggctttcatc gtgggtagct
    10621 tcttccggat cggcctgtcg gaagtgcggc ccgagcaggc cggcgccggc agcgcgacgc
    10681 tgtcgaccgt gcagcagtcg gcgctgggcc tggggccggc gctgctgggc gcggtgctga
    10741 cgcatttcct gaatacgcgc gaacacgact acctgaccg
//
LOCUS       DASWPO010000164         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2804802, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000164 DASWPO010000000
VERSION     DASWPO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2804802"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGC69_09015"
     CDS             complement(<1..359)
                     /locus_tag="VGC69_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF802 domain-containing protein"
                     /protein_id="HEY0295455.1"
                     /translation="MKNLLNPVVFLLGLLAVCWVGIGYLGSNPLGAAIALVIGACYIA
                     GALELYRYRQATATLKQAVAEVAPAQSDLGTWLGQLHPSLRNAVRLRIEGERIALPAP
                     ALTPYLVGLLVLLGMLGT"
     gene            complement(370..1110)
                     /locus_tag="VGC69_09020"
     CDS             complement(370..1110)
                     /locus_tag="VGC69_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3348 domain-containing protein"
                     /protein_id="HEY0295456.1"
                     /translation="MSKAPQRTPLPGPAFIRLLARLTDVDVPPSRHALSDRLSHWVDW
                     NRAIVLSRALDGSPAPGTDAPAFGSTEEAECARLRTSLAQAILEAPELNPTAQSPDPG
                     TADDPAPGPALDFSVFRKRYVARQQAMQTATGYLRGRLRDMLAAQSGERARLAEIDAV
                     MELTLSPREQTLLAAVPTLLGEHFERLRQGEAPPAQDDTPPPQDPTPAAQRAWLEGFR
                     RDMQQLLLAELDVRFQPIDGLLAALRTH"
     gene            complement(1287..>1984)
                     /locus_tag="VGC69_09025"
     CDS             complement(1287..>1984)
                     /locus_tag="VGC69_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0295457.1"
                     /translation="MGQQGYRVIAHDRRSHGRSDQTWTGNDMDTYADDLAAVLDTLDV
                     RDAILVGHSTGGGEIAHYVGRHGTRRVAKMVLVGAVPPLMLKTPGNPNGTPLEVFDGI
                     RAGTAGDRSQFFRDLSGPFFGANREGNTVTPGMRDHFWLQGMAGGIKGQYDCIHAFSQ
                     VDYTQDLEKINVPTLVIHGDDDQIVPIDAAGKLSSKIIRNAELKIYPGAPHGLTVTHA
                     DTFNADLLAFAKR"
BASE COUNT          299 a          625 c          740 g          320 t
ORIGIN      
        1 gtgcccagca tgcccagcag caccagcagg ccgaccaggt acggcgtcag cgcgggcgcg
       61 ggcagagcga tgcgctcgcc ctcgatgcgc aggcgcacgg cgttgcgcag gctcggatgc
      121 agctggccca gccaggtccc caggtcggat tgcgccggcg ccacctcggc cacggcctgc
      181 ttcagcgtgg cggtggcctg tcggtagcgg tacagctcca gggcaccggc gatgtagcag
      241 gcgccgatca ccagcgcgat cgccgcgccc agcgggttgg agccgaggta gccgatgccg
      301 acccagcaca cggccagcag gcccaggagg aagacgacgg ggttgagcag attcttcatg
      361 aggtcccagt tagtgggtgc gcagcgctgc aagcaggccg tcgatcggtt ggaaacgcac
      421 gtccagttcg gccagcagca gctgctgcat gtccctgcgg aatccctcca gccaggcgcg
      481 ctgggcggcc ggcgtcgggt cctgtggagg cggggtgtcg tcctgcgcgg gcggtgcttc
      541 accctggcgc aggcgttcga aatgttcgcc cagcaacgtg ggcacagcgg ccagcagggt
      601 ctgttcgcgc gggctcaatg tcagttccat caccgcgtcg atctcggcca ggcgcgcgcg
      661 ttcgcccgat tgcgcggcca gcatgtcgcg caggcgaccg cgcaggtagc cggtcgcggt
      721 ctgcatcgcc tgctgtcggg ccacgtagcg tttgcgaaaa acggaaaaat ccagcgccgg
      781 gccgggcgct ggatcgtcgg cagtgcccgg gtcgggcgat tgggccgtcg ggttcagctc
      841 gggcgcctcc aggatggcct gggccagcga ggtgcgcagg cgggcgcatt cggcttcttc
      901 ggtgctgccg aaggccggcg cgtcggtgcc gggagcgggg ctgccatcga gcgcccggga
      961 caggacgatg gcgcggttcc agtcgaccca atggctcagc cggtcggaca aggcatggcg
     1021 cgatggcggc acgtcaacgt cggtcaaccg ggcaagcagg cggatgaacg ccgggccagg
     1081 cagcggcgtc cgttgagggg ctttcgacat tggactggct aaaacgcgcc attttacacg
     1141 ccaatgacag tgcccgccgg actggggcag ggcgcggctc ccggcgagca gcggtccaag
     1201 ctgaacggac tggcgatgca ggtggacatg cggaaagggc cggcggttgt ccgctggccc
     1261 tcctggagtc ccgatggatt gaccgatcag cgcttggcga aggccagcag gtcggcgttg
     1321 aacgtatcgg catgggtgac ggtcaggccg tgcggggcgc cgggatagat cttcagttcg
     1381 gcgtttctga tgatcttcga ggacagcttg ccggccgcgt cgatcggtac gatctggtcg
     1441 tcgtcgccgt ggatgaccag ggtcggtacg ttgatcttct ccaggtcctg cgtgtagtcg
     1501 acttgcgaaa acgcatgaat gcagtcgtac tggcccttga tcccgccggc catgccttgc
     1561 agccagaagt ggtcgcgcat gcccggggtg acggtgttgc cctcgcggtt ggcgccgaag
     1621 aacgggccgc tcaggtcccg gaagaactgc gagcgatcgc cggcggtgcc ggcccggatc
     1681 ccgtcgaaca cctccagcgg ggtgccgttg ggattgccgg gggtcttgag catcagcggc
     1741 ggcaccgcgc cgaccaggac catcttggcc acgcgcctgg tgccgtggcg gccgacgtag
     1801 tgggcgatct cgccgccacc ggtggaatgc ccgaccagga ttgcgtcgcg cacgtccagc
     1861 gtgtccagca cggcggccag gtcgtcggca taggtgtcca tgtcgttgcc ggtccaggtc
     1921 tggtccgagc ggccgtggct gcggcggtca tgggcgatca cgcggtagcc ctgttggccc
     1981 atga
//
LOCUS       DASWPO010000165        16087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_749831, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000165 DASWPO010000000
VERSION     DASWPO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 16087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16087
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749831"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VGC69_09030"
     CDS             <1..1367
                     /locus_tag="VGC69_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0295458.1"
                     /translation="LAALLVLGGCGLPIARTGAGLDLPAAWQAPAAPGAAIDADWWRG
                     FGSAELSGLVETAGAGSFDVAAAAARVRQADALARVAGAALLPEVDGGASAERSKGVG
                     YPGLTSYTVGLSASYELDIWGANRAARQAAVDTLAATAYARDAVALTLTAGVADTYLQ
                     TLALRERAAIARQNLAAGERILAFVASQYRAGATTALDLAQQRTLAAQLRQAVAQRDQ
                     QARDSLTALATLIGRPPQGFAIDAAALGALAAPAMDAGVPSDLLARRPDVALAERQLA
                     AADADVTVARAAMLPAITLTASAGVGSDRLSGLFDHPIHDLAAGLAAPIFNAGRLAAG
                     RDLALAQREELLADYRKAIVNALGDVETALNAIDGVARQQQAQDEALAQAREAVRLAE
                     SRYRAGAETLLTLLDAQRTLYDAQDQAAQLRLARLQASVALYKALGGGWQIQHAHIVE
                     GDKP"
     gene            1364..1816
                     /locus_tag="VGC69_09035"
     CDS             1364..1816
                     /locus_tag="VGC69_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0295459.1"
                     /translation="MNVIEAFPIAPVLDRLRAAQLRTTAPRIAVLQALLSRPEDWFCA
                     ETLYRALIKRGVETSLGTVYRVMNEMAPLGLLLRRVDGNGKRFFRLRPDDHVLHRIVC
                     RDTGRLLAMTDDTSQGHLASVLRSQGLMLADAPMTVQVRCVEKTDQAM"
     gene            1860..2750
                     /locus_tag="VGC69_09040"
     CDS             1860..2750
                     /locus_tag="VGC69_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295460.1"
                     /translation="MNIRQLVSFVRVAELGSFAAAAKALNTTQSTISARIRELETAWG
                     VALFDRSHHRTQLSPKGQEILPWARQIIRLSDRIGYQLGEPEAFTGLLRIGVAGRIAH
                     TWLPRLVGAIRARHPRVRFDIRLGLTAPLLQQLKAGELDLAFAGGPITDPALDFRSLG
                     YDEFVWMASPLLELPETTLAPQDLARWPMVGLSQDSPHYPAIAQWFQASQIEYQPAIS
                     CNDMATAAKLIGAGLGVGLLHRASHRAEVLAGLLAVLDTAPPFPRLEFVAVYPRSGAS
                     PLALAVAELAMEISEFPRKT"
     gene            complement(2775..3752)
                     /locus_tag="VGC69_09045"
     CDS             complement(2775..3752)
                     /locus_tag="VGC69_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295461.1"
                     /translation="MRFIRSAALLAPAFCAVLLAPSGQAQAAYPDKPIKIIVPFTPGG
                     TSDVLTRTVGEQLGKDLGQSVVVENRAGAGGALGAGFVARAEPDGYTLLMGSSSGLAV
                     NPGLFSNLPYDPEKSFTPISMVAGIQNILLVNPSLPIKTVPDLIAYGKQHPLFFASAG
                     IGSSPHMSAEVFEAMSGLKMTHVPFKGGPESLSEVISGRVNLVFDNMPTAAVMVKSGQ
                     LRGVAVTGLNRSPMVPDLPTIDSQGLPGYNVTVWYGLVAPAGTPPAIIALLSGEVAKI
                     LARPEIKAKLIEIGTEPASSTPAEFKALIASERAKWADVIKKADIKVMN"
     gene            complement(3887..4933)
                     /locus_tag="VGC69_09050"
     CDS             complement(3887..4933)
                     /locus_tag="VGC69_09050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0295462.1"
                     /translation="MELHRQIDLDADEVVAIMEDRTVSANPKVRDTPLAGPIVWDRTT
                     LGPDDGIIPIGPACLAEIEAFLDDLRRNPLPTLLLDAADYDMPHCKAMMAKARDILHN
                     GVGFVILDKLPVASYSLEESRAVYWMLCQMSGRPVAQSFDGKMIYDVKNIERPYSTSV
                     RGDTTNRGQNFHTDNNYNLCPPHFVALFCLHPAKSGGINSIVSFYAAYNEMLKRYPMR
                     LVERLYHPYLANRQREHAPGDPMVLRRPLFTYDGAWMNCKLSKHQTLSGYQVAGQEID
                     PLGMEAIEALEAIMMEDRFNRDFFFERGQIQIVDNRRCGHRRTAFEDFEEPERKRHLL
                     RIWLRDEGRRSYLG"
     gene            complement(5114..5788)
                     /locus_tag="VGC69_09055"
     CDS             complement(5114..5788)
                     /locus_tag="VGC69_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEY0295463.1"
                     /translation="MKILIYGATGMVGQGVLRECLDAPDVASVVAIGRTPLGMRHPKL
                     QEIVRPDLYRYDGLETQLSGFDACFFCLGTSSAGKSEAVYTRITYDLTMAAASLLARL
                     NPDMVFTYVSGASTDSSEQGRAMWARVKGRTENALLKLPFKAAYMFRPGLIRATDGSV
                     SKTPAYRFLYGLLAWLIPLLHRAFPNQVVSTREIGKVMLSLARAPAGKPIYEIRDIRA
                     ALDRLG"
     gene            complement(5907..6830)
                     /locus_tag="VGC69_09060"
     CDS             complement(5907..6830)
                     /locus_tag="VGC69_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295464.1"
                     /translation="MKLEAFQTLAAVVGKGSFAAAAQAMHMTPSAVSMQMKQLEQYLG
                     KPLFDRSGQQVRMRPAALALVETMAPALNALSAMRRRTATEIEGHLQVGVIEGLQPLL
                     LPPAIRYLRDHYPRLEVSPQRGTSLALTEEVKAGRLDAAIAAKPPRGTQAGLRWIPLA
                     QQGLVFILPPAGSGAAPHAPRRLGEAELKRRIRTTDWIRYDRATTTGSLATRFVHKLV
                     PGKRSLMELGSAAAIAAMVNAGLGFSVLQLIDAGLVRQYPLEVFQLPRNGPVFELCVV
                     MRASDADDRLLQAWLEAVRHAMPGDGAAPSA"
     gene            6968..8074
                     /locus_tag="VGC69_09065"
     CDS             6968..8074
                     /locus_tag="VGC69_09065"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0295465.1"
                     /translation="MTDLASGWTSQQATEDASWTQRLSPAAVQGFDVALRHAKATGKA
                     LLDMEQADFPLPAVSRQALDLALQATQGPWSMCLLKGFPVDRWSEADVRLAYWGMGLY
                     LGVARTQNRASDILNDVRDAGGSYKTTNGRGYNTNAELDFHCDSCDVVALLCRRTAKE
                     GGASKVVNSRALVAEFGRRRPDLPAVLRQPFYYSYQGAQDPVQPPYYSIPLLGAESAP
                     FAFRINRKNVAAAQRDFDTVPRLSLAQTEALDLIDTLLEDPSLCYSMWLEKGDLQLLN
                     NYNVVHSRTRFVDFEDPDQKRHLLRLWLAVPISQRLPDDWAPYYGDNRAGAVRGGLRG
                     SNRTPAFEAFERRQAARMSMPFTPFVAPVAPAMA"
     gene            8200..9174
                     /locus_tag="VGC69_09070"
     CDS             8200..9174
                     /locus_tag="VGC69_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY0295466.1"
                     /translation="MNRRHFLAATLPAALALRALPARAQTGLDGHGAIRLEVGAPPGG
                     GTDTVARAMAMGMAPAIKRNIVVENRPGAGGNIAASTVAQAAGDASILLLAYTSHSIN
                     ASIQDNLPFDPLKSFTPVSLVATCPLILVCNPALPVSNTAELIDYARRNPGKLSMAGA
                     GLGSASQMSGEMLKAQAKLDIISVPYKGAAPAIQDILGGQVQMMLSDVAAVRPFIQSG
                     KLKALGVSVPEPIAAFPQLAPIDKVLPGFDYRIWYGLLGPAGLDPAQAATLEQAAQAA
                     VKTDAVSKHLVMEGLDPVGSTSVAFTDFIRKELARWKTVAQVTGVHLS"
     gene            complement(9270..9938)
                     /locus_tag="VGC69_09075"
     CDS             complement(9270..9938)
                     /locus_tag="VGC69_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEY0295467.1"
                     /translation="MKISIPRLLAFNGGYVDTAGFLALQGLFTAHVTGNFVTLGAALV
                     QGTSGIAAKLLALPVFCIMVLVVRFLSKPVEEGALRVLLVVKLLLFVAGAVMAIKFGP
                     FPDGDSAPALATGLTLVAAMAIQNAVHRIYFPKAPPSTLMTGSTTQIMLDAADLLRGG
                     DPAETRSATRARSLSMLYSVVIFAVGCGIAAVVYANLGMPVFLLPPVITALVLRLHLR
                     APRA"
     gene            10153..11739
                     /locus_tag="VGC69_09080"
     CDS             10153..11739
                     /locus_tag="VGC69_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY0295468.1"
                     /translation="MHNVEFFHYLLFLICGAGLLTWLAERIAIAPAVVLFAGGSAVAL
                     FGGQPAASMDPALVLMAILPPLLMSSAFYTAWGDFKRNGAAIASLALGAVVFTTVAVA
                     AAVRIADPSLPWPACFALGAIVSPPDAVAAKAMLQRFPLPARLVTVLEGESLVNDASG
                     LLLYQMAIAAALAGRFTAGTAASLFVTLTLVGIVTGVICGQVMMWLIRRLRDPMLCIL
                     LTFLMAWGSYAIAEALGGSGVLSVVACGLVLGVRQHKAFDAGMRIKAESTWETVVFVL
                     DALVFILIGLSLHALLARMQADGALLRAGLDVALPAVGAVVAARLLWVGLAVYLPSRL
                     TARPGGRPAWSFGESLVLGWAGMRGVVSLAAALALPEALPGRDVILFSTFVVIFATLV
                     VQGLTLAPLIRLLKLKPGHRAAAMNEHEVRSHTFRASLIELRHRRAANPAAQDEDLDR
                     LIGEYQIRVRNNENAHQHGQEHVDRRARQLRIELDLVGVSRQELLRLHRAGRVPDPVL
                     HRIESELDFEELRLQRLLAP"
     gene            complement(11812..12684)
                     /locus_tag="VGC69_09085"
     CDS             complement(11812..12684)
                     /locus_tag="VGC69_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0295469.1"
                     /translation="MQAHVDANGAYTKRFNAVLAYIEMNLEGDLSVDALSRVANFSKF
                     HFHRQFAAYVGVPVARYVQRMRLRRAAHRLASREFCSVLDAAFGAGFDSPEAFSRAFK
                     RAFGMAPSSFRRHPNWQIWSATFVVPYLSRKLTMQVHIVDFSETRVAALEHRGPTGLV
                     NESVRKFVDWRKQSGQSPIASSRTFGIPRNNPDATPADEFRFDICGEIDEPVAANAHG
                     IRELVIPGGRCAVVRHTGSTDHVGETIYPIYRDWLPASGEELRDHPLFFHYLSVFPET
                     PQDQWQTDIYIPLV"
     gene            complement(12806..14059)
                     /locus_tag="VGC69_09090"
     CDS             complement(12806..14059)
                     /locus_tag="VGC69_09090"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEY0295470.1"
                     /translation="MPNDYRASAYNGRINLEQQRKRAKALLTRLKDGTAAAQRNVLER
                     QAAFSGTFRLADAQWLIARELGFASWPALKAHADAVDFAARHPRFAADDEAGTQHWRC
                     GNDVAHSLRLAGFQGAFHMFADPLCMGPVPDVPDAEFLDMRSAFISHAFAMPLEDARR
                     RMHAEYQDLARISQATRAVLWCDADAYDQLFLIAILAGLKTSSGTLELIEIGHVPGVE
                     RFIGIGQLAPEVLAWLWPQRRRLDAGALALARTAWRAYRAASPQAWAELACGETPALP
                     FLAPALRRQLQELPSLRDGLSLSERLVLQIISQHDRPTLGQVFAELNGRREPLPFLGD
                     LMFHAVARSLIDASSPLIREFDSQHDWPHRPLELTGLGRRVLEGKAYWFDHTDIGRWV
                     GGVHIEPGRPHWALDEMLMPKWRRQ"
     gene            complement(14233..14892)
                     /locus_tag="VGC69_09095"
     CDS             complement(14233..14892)
                     /locus_tag="VGC69_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0295471.1"
                     /translation="MGHPGELKQARSIATRDALIAAALDLIYEDGYDAATTHAISQRA
                     QVSRGALLYHFPNKNDIIIAAMDRSMSEATDLIRGEARRVAARDSNIEEFVDFVWRMF
                     TKRFFYLAMEHITESRTNPELRERLLPRVRQFHEALDAVWVELYRDAPISARHAQVVL
                     NMTVCLIRGMGVQTVYRGDADYFDSMLRLWKSVLRGLVEGDLDAFIARAGLPISLRSS
                     P"
     gene            15162..15800
                     /locus_tag="VGC69_09100"
     CDS             15162..15800
                     /locus_tag="VGC69_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0295472.1"
                     /translation="MSTSDKYAERSFGAIPVGFGKRPGIVVVDFQIGYTHEGYPFGGS
                     PLIDRALANTQKLLEVARRLGVPVANCNTAYMDEREMPYWKITATRELMRHGNKAAEF
                     DPRIYDPGYDLAVCKKGASIFFDTTVASYFIKNQVDTVIVTGCNTSGCIRASVVDSFN
                     HRFRTIVPEDCVGDLDEGPHRDNLRDVERRYADVTDLKTCIEYLERCSAAAE"
     gene            15862..>16087
                     /locus_tag="VGC69_09105"
     CDS             15862..>16087
                     /locus_tag="VGC69_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295473.1"
                     /translation="MMTRRRALMQLGALPLAIGMPALVRAQGEQVYRIGALNPVTGQG
                     SAYGTGMQKMILAMADQINAAGGVLGRKVEV"
BASE COUNT         2646 a         5512 c         5311 g         2618 t
ORIGIN      
        1 cgctggccgc gctcctggtc ctgggcggct gcggcctgcc cattgcccgg accggcgccg
       61 gcctggactt gcccgccgcc tggcaagcgc cggcggcgcc gggcgcggcc atcgatgccg
      121 actggtggcg ggggttcggc agcgccgagc tgtccggcct ggtcgagacg gcgggcgccg
      181 gcagtttcga cgtggcggcc gcggccgcgc gcgtgcgcca ggccgatgcg ctggcgcgcg
      241 tcgcgggcgc cgccctgttg cccgaggtcg acggcggggc ctcggccgaa cgcagcaagg
      301 gggtcggcta tccggggctg acgtcctaca ccgtcggcct gtcggccagc tacgagctgg
      361 acatctgggg cgccaaccgc gccgcccgcc aggcggcggt cgataccctg gccgccacgg
      421 cctatgcccg cgatgccgtg gcgctgacgc tcacggcggg cgtggccgac acctacctgc
      481 aaacgctggc gctgcgcgag cgcgccgcca tcgcgcggca gaacctggcg gcaggcgaac
      541 gcatcctggc cttcgtcgca tcgcaatacc gggccggcgc gaccacggcg ctggacctgg
      601 cccagcagcg cacgctggcg gcccagctgc ggcaggccgt ggcgcagcgc gaccagcagg
      661 cgcgcgacag cctcaccgcc ctggcgaccc tcatcggccg cccgccccag ggattcgcga
      721 tcgacgcggc cgccttgggc gccctggcgg ccccggccat ggatgcgggc gtgccgtcgg
      781 acctgctggc ccgccgcccc gacgtggccc tggccgaacg gcagctcgcc gctgcggatg
      841 ccgacgtgac cgtggcgcgc gcggccatgc tgcccgcgat cacgctcacc gcctcggccg
      901 gcgtgggcag cgaccggctg agcggcctgt tcgaccatcc catccacgac ctggccgccg
      961 gcctggccgc gccgatcttc aacgccgggc ggctggccgc cgggcgcgac ctggccctgg
     1021 cccagcggga agaactgctg gccgattacc gcaaggccat cgtcaatgcc ctgggcgacg
     1081 tggaaacggc gctcaacgcc atcgacggcg tcgcccgcca gcagcaagcc caggacgagg
     1141 cgctggccca ggcccgcgag gcggtgcggc tggccgagtc ccgctaccgg gcgggggccg
     1201 agacactgct gacgctgctc gatgcccagc gcaccctcta cgacgcccag gaccaggccg
     1261 cgcagctcag gctcgcccgc ctgcaggcgt cggtggcgct ctacaaggcg ctgggcggcg
     1321 gctggcaaat acaacacgcc cacatcgtgg aaggagacaa gccatgaacg tgatcgaagc
     1381 attccctatc gcgccggtgc tcgacaggct gcgcgccgcg cagctgcgca cgacggcgcc
     1441 gcgcattgcc gtcctgcaag ccctgctaag ccggccggaa gactggtttt gcgcggagac
     1501 cctgtaccgc gcgctgatca agcgcggcgt ggaaaccagc ctgggcacgg tctaccgcgt
     1561 catgaacgag atggcgccgc tgggcctgct gctgcgccgg gtggacggca acggcaagcg
     1621 gttcttccgg ctgcggcccg acgaccacgt gctgcatcgc atcgtctgcc gcgacaccgg
     1681 ccgcctgctc gccatgaccg acgacacctc ccagggccac ctggccagcg tgctgcgcag
     1741 ccagggcctg atgctggccg acgccccgat gaccgtgcag gtccggtgcg tcgagaagac
     1801 ggaccaggcc atgtaatacg cgacttggcc ggatgacggg cgctataaac tggcaaccca
     1861 tgaacatccg ccaattggtt tccttcgtcc gcgtggccga gctgggcagc ttcgccgccg
     1921 cggccaaggc gctcaatacc acgcaatcga ccatctcggc ccgcatccgc gagctggaaa
     1981 cggcctgggg cgtggcgctg ttcgaccgca gccatcaccg tacccagctc agtcccaagg
     2041 gccaggaaat cctgccctgg gcgcggcaga tcatccggct gtccgaccgc atcggctacc
     2101 agttgggcga acccgaggcc ttcaccgggc tgctgcgcat cggcgtggcc gggcgcatcg
     2161 cccatacctg gctgcccagg ctggtcggcg ccatccgcgc ccggcacccg cgggtgcgct
     2221 tcgacatccg gctcgggctg accgcgccgc tgctgcagca attgaaagcc ggcgagctgg
     2281 acctggcctt tgccggcggg ccgatcaccg acccggcgct ggacttccgt tcgctgggct
     2341 acgacgaatt cgtctggatg gcctcgccgc tgctcgagct gccggaaacg acgctggcgc
     2401 cgcaagacct ggcccgctgg ccgatggtcg gcctgtcgca ggattcgccg cactacccgg
     2461 ccatcgcgca gtggttccag gcgagccaga tcgagtacca gcccgccatc tcgtgcaatg
     2521 acatggccac ggccgccaag ctgatcggcg ccgggctggg ggtgggcctg ctgcaccgcg
     2581 cgtcgcaccg ggccgaggtg ctcgccggcc tgctggccgt gctcgacacc gcgccgccct
     2641 tccctcgcct ggagttcgtg gccgtgtatc cgcgcagcgg cgccagcccg cttgccctgg
     2701 ccgtggcgga actggcgatg gaaatcagcg aattcccgcg caagacctga cgccgcgcgg
     2761 gcagtccatc gccgtcagtt catcactttg atgtcggcct tcttgatgac atcggcccac
     2821 ttggcccgct cggacgcaat cagcgccttg aattcggcgg gcgtcgagct tgccggctcg
     2881 gtgccgatct cgatgagctt ggccttgatc tcgggcctgg ccaggatctt cgccacttcg
     2941 ccgctcaata gcgcgatgat cgccggcggc gtgccggccg gcgccaccag gccgtaccag
     3001 acggtcacgt tatagcccgg cagcccctgc gaatcgatgg tcggcaagtc cggcaccatg
     3061 ggcgagcggt tcaggcccgt cacggccacg ccgcgcaact ggcccgactt gaccatgacg
     3121 gcggccgtgg gcatgttgtc gaacaccagg ttgacgcgcc cggagatcac ttcggacaag
     3181 gactcggggc cgcccttgaa gggcacgtgc gtcattttca ggccgctcat ggcctcgaac
     3241 acctcggccg acatatgcgg cgacgaaccg atgcccgcgc tggcgaagaa cagcggatgc
     3301 tgcttgccat aggcaatcag gtccggcacg gtcttgatcg gcagcgacgg gttgaccagc
     3361 aggatgttct ggatgccggc caccatcgag atgggcgtga agcttttctc ggggtcgtaa
     3421 ggcaggttgg agaacaggcc ggggttcacc gccaacccgg agctggagcc catcagcaag
     3481 gtatagccgt cgggctcggc gcgggccacg aagccggcgc cgagcgcgcc gcccgcgccc
     3541 gcgcggttct cgaccaccac ggactggccc aggtccttgc ccagttgttc gccgaccgtg
     3601 cgcgtcagca cgtcggaggt gccgcccggc gtgaacggca cgatgatctt gatgggtttg
     3661 tccggatagg ccgcctgggc ctggcccgac ggggcgagca atacggcaca gaaggccggc
     3721 gcaagcagcg ccgccgagcg gatgaaacgc atgagtattc ccttagtcgc gttatttgga
     3781 aggatggacg catcggtctc tctccagccc cccttcccca ccatgacggt ggcggccggc
     3841 attctatgtg ccagccaaaa tactcgcacc catatgctta tgctttttag cccaggtaag
     3901 agcgccggcc ctcgtcgcgc agccagatgc gcagcaggtg gcgcttgcgt tcgggctcct
     3961 cgaaatcctc gaaggccgtg cgccgatggc cgcagcggcg gttgtcgacg atctggatct
     4021 gcccgcgctc gaagaagaag tcgcggttga agcggtcttc catcatgatg gcctccagcg
     4081 cctcgatggc ctccatgccc agggggtcga tctcctggcc ggcgacctgg tagccgctca
     4141 gcgtctggtg cttggacagc ttgcagttca tccacgcgcc atcgtaggtg aagagcgggc
     4201 ggcgcagcac catgggatcg cccggcgcgt gctcgcgctg gcggttggcc aggtagggat
     4261 ggtagaggcg ctcgaccagc ctcatgggat agcgcttgag catctcgttg taggccgcgt
     4321 agaagctcac gatgctgttg atcccgccgc tcttggccgg atgcaggcag aacagcgcca
     4381 cgaaatgcgg cgggcagagg ttgtagttat tgtcggtgtg gaaattctgc ccgcggttgg
     4441 tggtgtcgcc ccgtaccgag gtgctgtagg gccgctcgat attcttgacg tcgtagatca
     4501 tcttgccgtc gaagctttgc gcgaccggcc ggccggacat ctggcacagc atccagtaca
     4561 ccgcccggct ctcctccagc gaatacgacg ccaccggcag cttgtcgaga atgacgaagc
     4621 ccacgccgtt gtgcaggatg tcgcgcgcct tggccatcat ggccttgcag tgcggcatgt
     4681 cgtagtcggc cgcgtcgagc agcagggtcg gcagcgggtt gcggcgcagg tcgtccagga
     4741 aggcctcgat ctcggccagg caggccgggc cgatggggat gatgccgtcg tcgggcccga
     4801 gcgtggtgcg gtcccacacg atgggccccg ccagcggcgt atcgcgaacc ttggggttgg
     4861 cgctgaccgt gcggtcttcc atgatggcga cgacttcgtc ggcgtcgaga tcgatctggc
     4921 ggtgcaattc catgatgcgg cgcagcttcc ttgttgcagt cttgtgaccc tctgggggga
     4981 atcgatcaag tataggaatg cgccccaccc ggccaaaatg aataattttg gtgttccacc
     5041 aacgaaaaaa acgatgctgc cgggccggcc cgccgggttg ggtctgcctg aaaagcgggc
     5101 cggcccgccg ccgtcacccc agcctgtcca gcgccgcccg gatgtcccgg atttcgtaga
     5161 tcggttttcc cgccggcgcg cgcgccaggc tcagcatgac tttgccgatt tcccgcgtgc
     5221 tgacgacctg gttcggaaag gcccggtgca gcaggggtat gagccaggcg agcaagccgt
     5281 agaggaatcg gtacgccggc gtcttggaga cggagccgtc ggtcgcccgg atcagccccg
     5341 gccggaacat ataggcggcc ttgaacggca atttcaggag cgcgttctcg gtgcgcccct
     5401 tgacgcgcgc ccacatggcc ctgccctgtt cgctgctgtc ggtgctggcc ccgctgacgt
     5461 aggtgaacac catgtccggg ttcaggcgcg ccagcaggct ggccgccgcc atcgtcaggt
     5521 cgtaggtaat gcgggtgtag acggcctcgc tcttgccggc cgagctggtg cccaggcaga
     5581 agaagcaggc gtcgaacccg ctcagttgcg tttccaggcc gtcgtagcgg tacaggtcgg
     5641 ggcgcacgat ctcctgcagc ttcggatgcc gcatgcccag cggcgtgcgt ccgatggcga
     5701 cgacggacgc cacatccggc gcatccaggc attcccgcag gacgccctgc ccgaccatgc
     5761 cggtggcgcc gtagatcagt attttcatgg tggctgccgc ctgtccggat aaaaattccg
     5821 gataaaaatg agtgagtgat taatcaatta taaggggctt caacgcgccg gcaaagcgcg
     5881 ctcccccgga acgcgtcgct tccagcctag gccgagggcg ccgccccgtc gcccggcatg
     5941 gcgtgccgga ccgcttccag ccaggcctgc agcagccggt cgtcggcatc gctggcgcgc
     6001 atgacgacgc acagttcgaa cacgggcccg ttcctgggca actggaagac ctccagcgga
     6061 tactgccgca ccaggcccgc gtcgatgagc tgcagcacgg aaaagcccag cccggcattg
     6121 accatggccg cgatggcggc ggcgctgccc agctccatca ggctgcgctt gccgggcacc
     6181 agcttgtgca cgaagcgcgt agccaggctg ccggtggtgg tggcccggtc gtagcgtatc
     6241 cagtcggtcg tccggatgcg gcgcttgagt tcggcctcgc ccaatcggcg cggcgcgtgc
     6301 ggcgccgcgc cgctgcctgc cggcggcaag atgaagacca gcccctgctg cgccagcggg
     6361 atccagcgca ggccggcctg cgtgccgcgg ggcggcttgg ccgcgatggc cgcgtcgagc
     6421 cggccggcct tgacctcctc ggtcaacgcc aggctggtgc cgcgctgggg cgacacctcc
     6481 agccgcggat agtggtcgcg caggtagcgg atggccggcg gcagcagcaa gggctgcaag
     6541 ccctcgatga cgccgacctg caaatgccct tcgatctcgg tggccgtgcg ccggcgcatg
     6601 gccgacaggg cattcaaggc cggggccatg gtctcgacca gcgccagcgc ggcgggacgc
     6661 atgcgcacct gctggccgga acggtcgaac aagggcttgc ccaggtattg ctcgagctgc
     6721 ttcatctgca tgctcaccgc gctgggcgtc atatgcatgg cctgggccgc cgcggcaaag
     6781 ctgcccttgc cgacgaccgc agccaaggtc tggaaggctt cgagtttcat caaatttttt
     6841 gatcttgaaa aacaaattaa gtcacttttt ctgctgactg gcaagacgta gactgcgaaa
     6901 catgcacggt aagtaccgct accgtggcgg caaacaaaat aagcagacaa cggagagaca
     6961 gaccatcatg accgacctag cctcgggctg gaccagccag caagccaccg aagacgcgtc
     7021 ctggacccag cggctgagcc ccgcagccgt gcagggattc gatgtcgcgc tgcggcatgc
     7081 caaggcaacg ggcaaggcgc tgctcgacat ggaacaggcc gactttcccc tgcccgccgt
     7141 atcgcgccag gcgctcgacc tggccctgca ggccacgcaa gggccctgga gcatgtgcct
     7201 gctcaagggc tttccggtgg atcgctggag cgaggccgac gtccgcctgg cctattgggg
     7261 catgggcctg tacctgggcg tggcgcgcac gcaaaaccgg gccagcgaca ttctcaacga
     7321 cgtgcgcgat gccggcggca gctacaagac cacgaacggc cgcggctaca acaccaatgc
     7381 cgagctcgac ttccactgcg actcgtgcga cgtggtggcg ctgctctgcc ggcgcaccgc
     7441 gaaagaaggc ggcgcgagca aggtcgtcaa ttcgcgcgcc ctggtggccg agttcggccg
     7501 ccgccgcccg gacctgccgg ccgtgctgcg ccagcccttc tactacagct accaaggcgc
     7561 ccaggatccg gtccagccgc cctactacag catccccctg ctgggcgccg aaagcgcgcc
     7621 tttcgccttt cgcatcaacc gcaagaacgt cgccgccgcg cagcgcgact tcgacacggt
     7681 gccgcgcctg agcctggcgc agaccgaggc cctggacctg atcgacaccc tgctcgagga
     7741 cccgtcgctg tgctattcga tgtggctgga aaagggcgat ctgcaactgc tcaacaacta
     7801 caacgtggtg cactcccgca cccgcttcgt cgacttcgag gaccccgacc agaagcgcca
     7861 cctgctgcgc ctgtggctgg cggtgccgat ttcccagcgg ctgcccgatg actgggcgcc
     7921 gtactacggc gacaatcgcg ccggcgcggt gcgcggcggc ctgcgcggca gcaaccgcac
     7981 cccggccttc gaagccttcg agcgccggca ggcggcccgc atgagcatgc cgttcacccc
     8041 tttcgtggcg cccgtggcgc cggccatggc ctagacgatt ggccgccctt catcccctat
     8101 taccaggccg cctcgaacga ggcggccttt atgccatgta ctgacgcaac acaagcttcg
     8161 catagcgggg ctacccctat ataaacaagg agacaaacca tgaaccgccg tcattttcta
     8221 gccgccacgc tgcccgccgc cctggcgttg cgcgccctgc ccgcccgggc ccagaccgga
     8281 ctggatggcc atggcgccat ccgcctggag gtcggcgcgc cgccgggagg cggcaccgac
     8341 accgtggccc gcgccatggc catgggcatg gcgccggcga tcaagcgcaa catcgtggtc
     8401 gagaaccgtc ccggcgccgg cggcaatatc gccgccagca cggtggccca ggcggcgggc
     8461 gacgccagca tcctgctgct ggcctatacc agccactcga tcaacgcgag catccaggac
     8521 aacctgcctt tcgatccgct caagagcttc acgccggtga gcctggtcgc gacctgcccg
     8581 ctgatcctgg tgtgcaatcc tgccctgccc gtctccaaca ccgccgagct gatcgattac
     8641 gcgcgccgca accccggcaa gctcagcatg gccggcgccg ggctgggcag cgccagccag
     8701 atgtccggcg aaatgctcaa ggcccaggcc aagctcgaca tcatcagcgt gccctacaag
     8761 ggcgccgcgc ccgccatcca ggacatcctg ggcggccagg tgcagatgat gctgtccgat
     8821 gtggccgccg tgcggccttt catccagagc ggcaagctca aggccctggg cgtgagcgtg
     8881 cccgagccga ttgccgcctt tccccagttg gcgccgatcg acaaggtgct gcccggcttc
     8941 gactatcgca tctggtatgg cctgctcggc cccgccgggc tggacccggc ccaggcggcc
     9001 accctggagc aggccgccca ggccgcggtc aagaccgatg ccgtgtccaa gcacctggtc
     9061 atggaagggc tcgacccggt cggcagcacg agcgtggcgt tcaccgattt catccggaaa
     9121 gaactcgcgc gctggaagac ggtcgcccag gtgaccggcg tgcacctgag ctgaaatcgg
     9181 ccggcgaact ggcgcggccg ggctttcgga tcgtgatctt ccccggcggc gctacgagca
     9241 aggccagccg ccggatccgg cgcgcatgct caggcccgcg gcgcgcgcaa gtgcagccgc
     9301 agcaccaggg ccgtgatcac cggcggcagc aagaagaccg gcatgccgag gttggcatag
     9361 accaccgcgg caatgccgca acccacggca aagatgacca cgctgtagag catcgaaaga
     9421 ctgcgggcgc gtgtagccga ccgggtctcg gccggatcgc cgccgcgcag caggtcggcc
     9481 gcgtcgagca tgatctgcgt cgtgctgccc gtcatcaagg tcgagggcgg cgccttggga
     9541 aaatagatgc ggtgcacggc gttctggatg gccatcgcgg ccaccagcgt caggccggtc
     9601 gccagggccg gcgcgctgtc gccgtcgggg aaaggcccga acttgatcgc catcacggcg
     9661 ccggccacga agagcagcag cttcacgacc agtaaaaccc gcagcgcgcc ttcctcgacc
     9721 ggcttgctca agaagcgcac caccagcacc atgatgcaga acaccggcag ggccagcagc
     9781 ttggccgcga tgcccgaagt gccttgcacc agggccgcgc cgagcgtcac gaagttgccc
     9841 gtgacgtggg ccgtgaacag tccctgcagc gccaggaagc cggcggtatc gacatagccg
     9901 ccattgaaag ccaacaacct cgggatcgaa attttcatat tctcgccttg acctcggtcg
     9961 gatcgcttgc ggccccatgg ccgccaggca agtgtactgt cgccggcccg gccgatttgg
    10021 tacatgcgta ccccgggatt gggacatgcg tgcccggccc cccatcgcgc cgacccgcga
    10081 tgtagcatag gcccgcatcc aatcatccgc ttgttgcctt cgttgccttc gttgctttcg
    10141 ccgcctgcgc ccatgcacaa cgttgaattc ttccactacc tgcttttcct gatctgcggc
    10201 gcgggattgc tgacctggct ggccgaacgc atcgccatcg cgccggcggt ggtgctgttc
    10261 gccggcggct cggccgtcgc cctgttcggc ggccagccgg cggccagcat ggacccggcc
    10321 ctggtgctca tggcgattct cccgcccctg ctgatgtcca gcgccttcta tacggcctgg
    10381 ggagacttca agcgcaatgg cgcagccatt gcctcgctgg cgctgggggc ggtggtcttc
    10441 accaccgtgg cggtcgcggc ggccgtgcgc atcgccgatc cctccctgcc ctggccagcc
    10501 tgctttgcgc tgggcgccat cgtctcgccg cccgatgccg tcgcggccaa ggccatgctg
    10561 cagcgctttc cgctgccggc gcgcctggtg accgtgctgg aaggcgaaag cctggtcaac
    10621 gatgcctccg gcctgctgct ctaccagatg gccattgccg ccgcgctggc cgggcgattc
    10681 acggcgggca ccgccgcttc gctgttcgtc acgctgacgc tggtgggcat cgtgaccggc
    10741 gtgatctgcg gccaggtcat gatgtggctc atccggcgcc tgcgcgaccc catgctctgc
    10801 attctgctga cctttctcat ggcctggggc agctacgcca tcgccgaggc gctgggcggc
    10861 tcgggcgtgc tgtcggtggt ggcgtgcggg ctggtcctgg gcgtgcgcca gcacaaggcc
    10921 ttcgatgccg gcatgcgcat caaggccgag tccacctggg aaaccgtggt cttcgtgctg
    10981 gacgcgctgg tgttcatcct gatcggcctg tccctgcacg ccctgctggc gcgcatgcag
    11041 gccgacggcg ccttgttgcg cgccggtctg gacgtggccc tgccggcggt gggcgcggtc
    11101 gtggcggcgc gcctgctgtg ggtgggcctg gcggtctacc tgccgtcgcg gctgaccgcc
    11161 cgccccgggg gacggccagc ctggtccttc ggcgaaagcc tcgtgctggg ctgggccggg
    11221 atgcgcggcg tggtcagcct ggccgccgcc ctggccctgc ccgaagcatt gcccggccgc
    11281 gacgtcattc tctttagtac cttcgtcgtc atcttcgcca cgctggtcgt gcaggggctc
    11341 acgctggccc cgctcatccg cctgctcaag ctcaagccgg ggcatcgggc cgccgctatg
    11401 aacgaacacg aagtccgctc gcacaccttc cgcgcctcgc tgatcgagct caggcaccgg
    11461 cgcgccgcca acccggcggc gcaagacgag gacctggacc ggctcatcgg cgaataccag
    11521 atccgcgtgc gcaacaacga gaacgcccac cagcatggac aggagcacgt ggaccggcgc
    11581 gcccggcagc tgcggatcga actcgacctg gtcggcgtgt cgcggcagga gctcctgcgc
    11641 ctgcaccgcg ccggccgcgt gcccgacccc gtcctgcacc gcatcgagtc ggagctggat
    11701 ttcgaggaac tgcggctgca gcggctgctg gcgccttgag ccgtatgccc accattggat
    11761 tgtgccgtcc ggcttgactc gcccatcgaa tgggcacagc gttccggtcc ctcacacaag
    11821 cggaatatag atgtcggttt gccactgatc ctgcggcgtt tccggaaaga cgctcaggta
    11881 gtgaaagaac agggggtggt cccgcagctc ttctccgctt gcgggcagcc aatcgcgata
    11941 gatcggatag atggtctcgc cgacgtggtc ggtcgagccg gtatgccgga ccaccgcaca
    12001 gcggccgccc ggaatgacga gctcgcgaat gccgtgggcg ttcgcggcga ccggctcgtc
    12061 gatttctccg cagatgtcga agcggaattc atccgccggc gtcgcgtccg gattattgcg
    12121 cgggatgcca aacgttctgc tcgacgcgat cggagattgc ccgctctgct tgcgccagtc
    12181 gacgaacttg cgcacgctct cgttgacgag cccggtcggc ccgcggtgtt caagcgcggc
    12241 gacgcgggtt tcggaaaaat cgacaatgtg cacttgcatg gtgagcttcc ttgaaagata
    12301 ggggacgaca aacgtcgcgc tccagatctg ccagttcgga tgccgccgga acgaactcgg
    12361 cgccatgccg aacgcgcgct tgaacgcgcg gctgaaggct tcggggctgt cgaatcccgc
    12421 gccgaacgcg gcgtcgagca ccgagcagaa ctcgcgcgat gccaggcgat gggccgcccg
    12481 ccgcaagcgc atccgttgca cgtagcgcgc aaccggcacc cccacatagg cggcaaactg
    12541 gcggtgaaaa tggaacttcg agaagttcgc aacgcggctc agcgcgtcca cggacaggtc
    12601 gccctcgagg ttcatctcga tgtatgcgag tacggcattg aagcgcttcg tgtaagcgcc
    12661 attggcgtcg acatgagctt gcatggcctg gaatctggat ggtgcgactt gcgttatggt
    12721 cgcccatgat cgaaccatcg cgcctagccg cgcttgctcg atcggggggt cgaatgactg
    12781 ctttcgacta cggattcgcc agtagtcatt ggcgacgcca cttcggcata agcatttcat
    12841 cgagcgccca atgaggacgg ccgggttcga tatgcacgcc gccgacccag cgcccaatat
    12901 cggtgtgatc gaaccaatag gccttgcctt ccagaacccg tcggcccaac ccggtcagtt
    12961 cgagcggccg atggggccag tcatgctggc tatcgaattc tcgtatcagc gggctggatg
    13021 catcgatcag ggagcgcgcc accgcatgga acatcaagtc gcccaaaaaa ggcaatggct
    13081 cgcggcggcc gttcagttca gcgaagactt ggcccaaggt cggccgatca tgctggctga
    13141 tgatctgcag cacaagccgc tcgctcaggg acaggccgtc gcgcagggac ggcagctcct
    13201 gcaattggcg acgcagcgcc ggggccagaa acggcagcgc gggggtctcg ccgcaagcaa
    13261 gttcagccca ggcttgcggc gaggcggcgc gataggcccg ccacgccgtg cgcgcgagcg
    13321 ccagggcgcc tgcgtccagc cgccgccgct ggggccagag ccaggcgagc acctccggcg
    13381 ccaattgtcc gatgccaata aagcgttcga cgccgggaac gtgaccgatc tcgatcagtt
    13441 ccagcgtgcc ggacgaagtc ttcagcccgg ccaggatcgc aatcaggaat agctggtcgt
    13501 aagcgtcggc atcgcaccag agcacagcgc gagtcgcttg ggatatccgg gccaggtcct
    13561 ggtactcggc atgcatgcgc cgccgggcgt cctcgagggg catagcgaag gcatggctga
    13621 tgaaggcact gcgcatgtcg aggaattcgg catcgggcac atccggtacc ggccccatgc
    13681 acagcggatc cgcgaacata tggaacgcgc cctggaagcc ggcgaggcgc aagctgtggg
    13741 caacgtcgtt gccgcagcgc cagtgttgcg tgccggcctc gtcgtctgcg gcaaaccggg
    13801 gatgccgggc ggcgaagtca acggcatcgg catgcgcttt gagtgcgggc cagctcgcga
    13861 aaccaagctc gcgcgcgatc agccactgcg cgtccgccaa tcgaaaagtg ccagagaagg
    13921 cggcttggcg ctcgaggacg ttgcgttggg cagccgccgt gccgtccttg agccgcgtca
    13981 gcagcgcctt ggcgcgcttg cgttgttgct cgaggttgat gcgaccgttg taggccgagg
    14041 cgcgatagtc attgggcata cgatctcctg ggtcggccgt gtccgctgct tggccgggag
    14101 tcgcatgtga aaaccgagtg atctggaacc tgggcgcagc cctttccgcg gacggggggc
    14161 gcggaagagc gcccagcgga aattctacaa cgagcgcccg gatcttgagc tgctatatcc
    14221 tgcccgtccc cgtcatgggg agctccgcaa tgagatgggc agcccggcac gcgcgatgaa
    14281 cgcgtccagg tccccttcca ccaagccccg cagcacggac ttccacagcc gcagcatgga
    14341 gtcgaagtaa tcggcgtccc cgcggtagac ggtctgcacg cccatgccgc ggatcaggca
    14401 gaccgtcatg ttgagcacga cctgcgcgtg gcgggccgag atgggcgcgt cccggtacag
    14461 ttcgacccag acggcatcca gggcctcgtg gaattgccgc acgcgcggca gcaggcgctc
    14521 gcgcaactcc ggattggtgc gcgattcggt gatgtgctcc atcgccaggt agaagaaccg
    14581 cttcgtgaac atgcgccaga cgaagtccac gaattcctcg atgttcgagt cgcgcgccgc
    14641 cacgcgccgg gcctcgccgc ggatcaggtc ggtcgcctcg ctcatcgagc ggtccatcgc
    14701 cgcgatgatg atgtcgttct tgttggggaa gtggtagagc agcgccccgc gggaaacctg
    14761 ggcccgctgg ctgatcgcat gggtagtggc cgcgtcgtac ccgtcttcgt agataaggtc
    14821 gagcgcggcg gcaatgagcg cgtcgcgcgt cgcaatgctc cgggcctgct tcagttcgcc
    14881 ggggtgtccc atcgatctcc tcctttttgt cctggccggc accgccgcgc gcgggcgcaa
    14941 tgataccgga ccgatgccgc cggcccacgc cgccagccgg ctggccgatt attgttttga
    15001 accataaaaa atcactcgtg atttttattt tttaataagc tgacgagata attatataaa
    15061 aacatagggg aaattactta tcctccataa aaacaaacag ccttgactgt ttttttctcg
    15121 attcatagaa tcgtccactt actgcaacag gagagcggca catgagcacc agcgacaaat
    15181 atgcggaacg cagcttcggg gcaatccccg tcggcttcgg caagcgcccc ggcatcgtgg
    15241 tggtggactt ccagatcggc tacacccacg aaggctaccc gttcggcggc tcccccctga
    15301 tcgaccgggc gctggcgaac acgcagaagc tgctcgaggt cgcccgccgt ctcggcgtgc
    15361 ccgtggccaa ctgcaacacc gcctacatgg acgaacgcga gatgccctac tggaagatca
    15421 ccgcgacccg ggagctcatg cgccacggca acaaggccgc cgaattcgac ccgcgcatct
    15481 acgaccccgg ctacgacctc gcggtctgca agaagggcgc gtcgatcttc ttcgacacca
    15541 ccgtggccag ctacttcatc aagaaccagg tggacaccgt catcgtcacc ggctgcaaca
    15601 ccagcggctg catccgcgcc tcggtggtcg acagcttcaa ccatcgcttt cgcaccatcg
    15661 tgccggaaga ctgcgtcggc gacctggacg aaggcccgca ccgcgacaac ctgcgcgacg
    15721 tcgaacgccg ctacgcggac gtcaccgacc tgaagacctg catcgagtac ctcgagcgct
    15781 gctcggccgc ggccgagtaa gccgtcccgg gcaagcagcc ctctgcccct gtttcttcct
    15841 atcgttcgtt tggagaatgc catgatgacc cggcgccgcg cgctcatgca gttgggagcc
    15901 ctgcctctcg ccatcggaat gcctgcgctg gtgcgcgcgc agggtgaaca ggtctaccgg
    15961 atcggcgcgc tgaaccccgt gaccggccag ggcagcgcct acggcaccgg catgcagaag
    16021 atgatcctgg ccatggccga ccagatcaac gccgcgggcg gcgtgctggg ccgcaaggtc
    16081 gaggtct
//
LOCUS       DASWPO010000166         5556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_305069, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000166 DASWPO010000000
VERSION     DASWPO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305069"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VGC69_09110"
     CDS             complement(<1..1448)
                     /locus_tag="VGC69_09110"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0295474.1"
                     /translation="MPDIPNLPLLIGGKLIQSKTTQWRDVINPATQKVVARVPMATRE
                     ELDLAVANAKDAYHDWRGSGQGTRMRVMLTLQQLLRENAGKLAEMITREHGKTLPDAE
                     GEVGRGLEVVEHACSIASLQLGEYAENAAGGIDVYTLVQPLGVCAGITAFNFPVMLPC
                     FMFPVAVACGNTFVLKPSEQDPSASLFLAQLALEAGLPPGVLNVVHGGPDIANGLCEH
                     PDVKAVSFIGSTRVGTEIYNRSSKAGKRCQAMMGAKNHCVILPDADPEVALNQLLGAA
                     FGAAGQRCMAASVAVLVGEARNWLPEFVARAKKLKVNAGIDRQADLGPLVSAAARKRV
                     EGLIQQGVDEGAELLLDGRNIKVPGFEQGNFVGPTVFGGVTGKMALYKEEIFGPVLCV
                     VGVETLEEAVAFINRNPNGNGVALFTQDGAAARYFQNNIDVGQVGINIPIPVPVAWFS
                     FTGSRGSKLGDLGPNGKQAVQFWTQTKTVTAR"
     gene            complement(1584..2690)
                     /gene="lptF"
                     /locus_tag="VGC69_09115"
     CDS             complement(1584..2690)
                     /gene="lptF"
                     /locus_tag="VGC69_09115"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY0295475.1"
                     /translation="MSLFKRSVVSEITSHGGVVFSTLLIVWISVLLVRLLGEAAAGNI
                     GADVVMGMAALSTITALPIILLVSLFIAVLTTVTRNYRESEMVVWFASGLSLSDWLKP
                     VLTVAAPVAVAVAWFTMQAAPWSYRQMGEYRERFEQRSDLSKVSAGQFAESSGGQRVF
                     FAEDPTKPGDELGNVFARVIDQDWHSIVTAHSAHTETMPNGDRFVVLTQGHRYDLKPG
                     TPEIRLVNFERYGLRLESRSSKDPETEAKEAAERSSKARTTWQLMTDNTLETWSQVMW
                     RVSMPLAALNLALLAIPLGAVNPRLGRSGDLLIAGLVAMLYMNLINLSRAWISDGKVS
                     FGLGVWLIHALVTGLFVFLLLRRLRVKAPKQSKP"
     gene            2796..4310
                     /locus_tag="VGC69_09120"
     CDS             2796..4310
                     /locus_tag="VGC69_09120"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0295476.1"
                     /translation="MEFSTQTTASPHQIKAAALAVGVYADGQLSPAADVVDRATGGGL
                     RAVVKAEFRGRAGSTLVLRALPGVSAQRVVLVGLGKRDEYNARAHAHAEQAFAAYCVA
                     ARLAEGVSTLAANDIADVSIRDRARAAAIAAGNAAYHYDATLGKPDAEARPKLKKVVQ
                     VVERADATAVQQGLKEGAAIARGMSLAKTLGNLPGNLCTPTYLGEEAKKLARDFKSIK
                     AQVLEQKQIEALGMGSFLSVARGSYEPPRFIVLRKPAAVAAKAKGKGKGPVGPVVLVG
                     KGITFDSGGISIKPAATMDEMKFDMCGAASVLGTFRALAELDLPYEVIGLIASCENMP
                     SGRANKPGDVVTSMSGQTIEILNTDAEGRLILCDALTYAERFKPAAVIDIATLTGACV
                     VALGSVNSGLFSKDDALAGALLAAGKQALDPAWRMPLDDAYQEQLKSNFADIANIGGP
                     KAGAVTAACFLSRFTKAYPWAHLDVAGTAWKSGGDKGSTARPVPLLAQYLLNRA"
     gene            4307..4756
                     /locus_tag="VGC69_09125"
     CDS             4307..4756
                     /locus_tag="VGC69_09125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY0295477.1"
                     /translation="MMRIDFAFGAPDRLRSACQTARKRHLAGQRLVVYCGDGARLSAF
                     DRMLWAFDDTSFVPHVLATDPLAPDTPVLLTAADPAVALPADQAASAWLINLDDECPP
                     SYDGYARLLEFVSGDAADRQSARQRWRIYQAAGHDLHGHDLTQSPEA"
     gene            4758..5021
                     /locus_tag="VGC69_09130"
     CDS             4758..5021
                     /locus_tag="VGC69_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295478.1"
                     /translation="MTTPYSTDGIPTLTQRAEPDIPLLDTPAVPLPTAGTIPEAVLRA
                     ALQADIEQAVKTAVDEASSLLRSRLETELPVILAQAMARVKPG"
     gene            5209..>5556
                     /locus_tag="VGC69_09135"
     CDS             5209..>5556
                     /locus_tag="VGC69_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEY0295479.1"
                     /translation="MNDYRPSPLNRSGSDAYAGAPGEVVRNRVLRNTYWLLALSMVPT
                     VLGAAVGLSTGLNLTMGASPGISAIIFLVGAFVLMMAIERNKNSSMGVALLLVFTFFM
                     GVMLSQLLGFVLGK"
BASE COUNT          916 a         2029 c         1753 g          858 t
ORIGIN      
        1 cagcgggccg tcaccgtctt ggtctgcgtc cagaactgca cggcctgctt gccgttgggc
       61 cccagatcgc ccagcttcga accgcgtgag ccggtgaagc tgaaccaggc caccggcacc
      121 gggatgggaa tattgatgcc cacctggccc acgtcgatat tgttctggaa gtagcgcgcc
      181 gccgcgccgt cctgggtgaa gagcgccacg ccattgccat tggggttgcg gttgatgaaa
      241 gccaccgcct cctccagggt ttccaccccc accacgcaca gcaccggccc gaagatttcc
      301 tccttgtaga gggccatctt gccggtgacg ccgccgaaca ccgtgggccc gacgaagttg
      361 ccctgctcga aacccggcac cttgatgttg cggccgtcca gcagcagttc cgcgccttca
      421 tccacgcctt gctggatcag cccctccacg cgcttgcggg cggcggccga caccagcggc
      481 cccagatcgg cctgccggtc gatgccggcg ttgaccttga gctttttcgc gcgcgccacg
      541 aattccggca gccagttgcg cgcctcgccc accagcaccg ccacggaagc cgccatgcag
      601 cgctggccgg ccgcgccgaa ggccgcgccc agcagctggt tgagcgccac ctccgggtcg
      661 gcgtcgggca ggatcacgca atggttcttg gcgcccatca tggcctggca gcgcttgccc
      721 gccttggatg agcggttgta gatctcggtg cccacccggg tcgagccgat gaacgacacg
      781 gccttgacgt cgggatgctc gcacaggccg ttggcgatgt ccgggccgcc gtgcaccacg
      841 ttgagcacgc ccggcggcag gcccgcctcg agcgccaact gggccaggaa cagcgacgcg
      901 ctcggatcct gttcggaagg cttgagcacg aaggtattgc cgcaggccac cgcgacggga
      961 aacatgaagc acggcagcat caccggaaag ttgaaggccg tgatgccggc gcacacgccc
     1021 agcggctgga cgagcgtgta gacatcgatg ccgcccgcgg cgttctcggc gtattcgccc
     1081 aattgcaggc tggcaatgga acaggcgtgc tcgaccactt ccagcccgcg gcccacttcg
     1141 ccctcggcgt cgggcagggt cttgccgtgc tcgcgggtga tcatctcggc cagcttgccc
     1201 gcgttctcgc gcagcagctg ctgcagcgtg agcatcacgc gcatgcgcgt gccctggccg
     1261 ctgccgcgcc agtcgtggta ggcgtccttg gcattggcca cggccaggtc gagttcttcg
     1321 cgcgtggcca tcggcacccg cgccaccact ttctgggtgg cggggttgat cacgtcgcgc
     1381 cactgcgtgg tcttggactg gatgagcttg ccgccgatca atagcggcag attgggaatg
     1441 tcaggcatgg cttgtctccg gattttttgc gcgccgctcc tgcggcgtct tcatccagtg
     1501 taggggtgcg gcaagcccgc agcaagcatc cagcccaacc ccaacccaac ccaaccccgg
     1561 cccgaccccg gccaaaaacg ggtctacggc ttggactgct tcggcgcctt cacgcgcagc
     1621 ctgcgcagca gcaggaacac gaacaacccc gtcaccaggg cgtggatcag ccacacgccc
     1681 aggccgaaac tcaccttgcc gtcgctgatc caggcacgcg agaggttgat gaggttcatg
     1741 tagagcatgg ccaccaggcc ggcgatcagc aggtcgccgg agcggcccag gcgcggattc
     1801 accgccccca gcggaatggc cagcagcgcc aggttgagcg ccgccagcgg catggaaacg
     1861 cgccacatca cctgcgacca ggtctcgagc gtgttgtcgg tcatcagctg ccaggtggtg
     1921 cgcgccttgc tggagcgctc ggccgcctcc ttggcctcgg tttccgggtc tttcgacgag
     1981 cggctttcca ggcgcagccc gtagcgctcg aagttcacca gccggatctc cggcgtgccg
     2041 ggcttgaggt cgtagcgatg gccctgggtc agcaccacga aacggtcgcc gttgggcatg
     2101 gtctcggtat gcgcgctgtg cgccgtgacg atgctgtgcc agtcctggtc gatgacgcgg
     2161 gcgaagacgt tgcccagctc gtcgcccggt ttggtcgggt cctcggcgaa gaacacgcgc
     2221 tgcccgccgg acgactcggc gaactgcccg gccgagacct tggacaggtc ggagcgctgc
     2281 tcgaagcgtt cgcggtactc gcccatctgc cggtacgacc agggagcggc ctgcatcgtg
     2341 aaccaggcca cggcgaccgc cacgggcgcc gccacggtca gcacgggctt gagccagtcg
     2401 gacagcgaca gcccgctggc gaaccacacg accatttcgg attcgcggta attgcgcgta
     2461 accgtcgtca gcaccgcgat gaagagcgac accagcaaaa tgatgggcag cgccgtgatg
     2521 gtcgacaggg ccgccatgcc catcactacg tcggcgccga tattgccggc cgcggcctcg
     2581 cccagcaggc gaaccagcag cacgctgatc cacacgatca acagggtgga gaacaccacg
     2641 ccgccgtggc tcgtgatctc gctgacgacg gatcgcttga ataaagacat gtgagaatat
     2701 ttggggggat aatcccgcaa gacatcggtc ggctgaccga tgcatccagc taaccatacg
     2761 cggaccggct tcggcccgcc acggaaaaca gagtcatgga atttagcaca cagaccaccg
     2821 cgtccccgca ccagatcaag gccgccgcgc tggcggtcgg cgtctacgcc gacggccagc
     2881 taagccctgc cgcggacgtg gtcgaccgcg ccaccggcgg cggcctgcgc gccgtcgtca
     2941 aggccgagtt ccgcggccgc gccggcagca cgctggtgct gcgcgcgctg ccgggcgtgt
     3001 cggcccagcg cgtggtgctg gtgggcctgg gcaagcgcga cgaatacaac gcccgcgccc
     3061 acgcccatgc cgagcaggcc ttcgccgcct actgcgtcgc ggcccgcctg gccgagggcg
     3121 tctccaccct ggccgccaac gacatcgccg acgtgtccat ccgcgatcgc gcccgcgccg
     3181 cggccatcgc ggccggcaac gccgcctatc actacgacgc caccctcggc aagcccgacg
     3241 ccgaggcccg ccccaagctc aagaaggtcg tccaggtggt cgaacgcgcc gacgccacgg
     3301 ccgtccagca gggcctgaag gaaggcgccg ccatcgcgcg cggcatgagc ctggccaaga
     3361 ccctgggcaa cctgcccggc aacctatgca cgcccaccta cctgggcgaa gaggccaaga
     3421 agctggcccg cgatttcaag tccatcaagg cgcaggtgct cgagcagaag cagatcgagg
     3481 cgctgggcat gggctccttc ctgtcggtgg cgcgcggctc ctacgaaccc ccgcgcttca
     3541 tcgtgctgcg caagccggcc gccgtcgcgg ccaaggccaa gggcaagggc aagggcccgg
     3601 tgggcccggt ggtgctggtg ggcaagggca tcaccttcga ctcgggcggc atctcgatca
     3661 agccggccgc caccatggac gaaatgaagt tcgacatgtg cggcgccgcc agcgtgctcg
     3721 gcaccttccg cgccctggcc gagctcgacc tgccttacga agtcatcggc ctgatcgcca
     3781 gctgcgagaa catgcccagc ggccgggcca acaagccggg cgacgtggtc accagcatgt
     3841 cgggccagac catcgagatc ctcaacaccg acgccgaggg ccgcctcatc ctgtgcgacg
     3901 ccctcaccta cgccgagcgc ttcaagccgg ccgccgtgat cgacatcgcc accctcaccg
     3961 gcgcctgcgt ggtggccctg ggcagcgtca acagcggcct gttctcgaaa gacgacgcct
     4021 tggcgggcgc cctgctggcc gccggcaagc aggcgctcga cccggcctgg cgcatgccgc
     4081 tggacgacgc ctaccaggag cagctcaagt ccaacttcgc cgacatcgcc aacatcggcg
     4141 gccccaaggc cggcgccgtc acggccgcct gcttcctgtc gcgcttcacc aaggcctatc
     4201 cctgggcgca cctggacgtc gccggcacgg cctggaaaag cggcggcgac aagggctcca
     4261 cggcccggcc ggtcccgctg ctcgcgcagt acctgctcaa ccgggcttga tgcgcatcga
     4321 tttcgccttc ggtgcgccgg accgcctgcg ctcggcctgc cagaccgccc gcaagcggca
     4381 cctggcgggc cagcgcctgg tcgtctactg cggcgacggc gcccggctct cggccttcga
     4441 ccgcatgctg tgggccttcg acgacacgtc cttcgtgccg cacgtgctgg ccaccgaccc
     4501 gctggcgccc gacacgcccg tcctgctcac ggcggccgac cccgccgtcg cgctgcccgc
     4561 cgaccaggcg gcctcggcct ggctcatcaa cctggatgac gagtgcccgc ccagctatga
     4621 tggctatgcg cgcctgctgg aattcgtctc cggcgacgcg gccgaccgtc aatcggcccg
     4681 ccagcgctgg cgcatctacc aggcggccgg ccacgacctg cacggccacg acctgaccca
     4741 gtcgcccgag gcctgagatg actacgccct actccaccga cggcatcccc acgctgacgc
     4801 agcgcgccga gcccgacatt cccctgctcg acacgccggc cgtcccgctg cccaccgccg
     4861 gcaccattcc cgaggccgtg ctgcgcgccg ccctgcaggc cgacatcgaa caggcggtca
     4921 agaccgccgt ggacgaagcc tccagcctcc tgcgcagccg cctggagacc gaactgcctg
     4981 ttatcctggc ccaggccatg gcccgggtaa agccgggctg aaacgagata aaacagggcc
     5041 acacctcttt gcccaggccc tgccattgaa accagccgca atatccccat cttcatcctg
     5101 acagcattca gtggaattta aggaaactgt caggcatact ggatgtctga agctggtggg
     5161 catcgtgccc gcctcgcttt catccgcata cctcctagag gaaccgtcat gaacgattac
     5221 cgtccttccc ccctcaaccg ttccggtagc gacgcctacg ccggcgcgcc gggcgaggtc
     5281 gtccgtaacc gcgtgctgcg caacacctac tggctgctcg ccctgtcgat ggttcccacc
     5341 gtgctgggcg ccgccgtggg cctgtccacc ggcttgaacc tgaccatggg cgccagcccc
     5401 ggcataagcg ccatcatctt cctcgtcggc gccttcgtgc tgatgatggc catcgaacgc
     5461 aacaagaaca gctcgatggg cgtggccctg ctgctggtct tcaccttctt catgggcgtc
     5521 atgctctcgc agttgctggg cttcgtgctg ggcaag
//
LOCUS       DASWPO010000167        11511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_484449, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000167 DASWPO010000000
VERSION     DASWPO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484449"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VGC69_09140"
     CDS             complement(<1..1463)
                     /locus_tag="VGC69_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0295480.1"
                     /translation="MLRHALAPLFEPGSLVVVADRDLPVLAALPAGLRARVTVVECEP
                     GLAPVPPGTCQGLAPGERPDLALVCVSPAVLPETLRRLGGLAPRGVIVLPHELPDPYP
                     GGTRALCEAWAEQHDCVLLGPRSFGVQRPHSQLNLSQHPTLALAGRVALVTQSRSIAA
                     AIMDWAEDVHIGFSTVVSLGDEAVVGLTQTLDFLASDPRTDSIVLYLEEAGPAREFMS
                     ALRAAASVKPVIVLKTGRGDDGGDAVFDAVLRRAGAVRVRYFVQLFSAVKVLGHVRRP
                     RGRRVAVLSNGNGPPQLAMDLIGPDGPTLRAELAPGTRRALQDMLEPDAATNNPVISY
                     VPLTPDRIQGLLDNLLADVGVDGVLVLLAPDALADMPAVAGRLSQIAPKARKPVISCF
                     MGDAGMRPLRRMLDDAGTPAFRTPESAVDAFGVLASYNYNQQLLQQTQPSEPTELPPD
                     IAGARAVIAGARAAGRRQLDTAEAQALLDAFQVPLSA"
     gene            1591..2997
                     /gene="gltX"
                     /locus_tag="VGC69_09145"
     CDS             1591..2997
                     /gene="gltX"
                     /locus_tag="VGC69_09145"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0295481.1"
                     /translation="MTAQNPSVVRTRFAPSPTGFLHLGGARTALFSWAFARHHQGTFV
                     LRIEDTDVERSTQAAVDAILDSMAWLGMQPDEGPFYQMQRMDRYREVLAGMLQEGTAY
                     HCYSSPEEVEAMREAARAKGLKPRYDGTWRPEPGKALPPIPAGRKPVVRFKNPQDGAT
                     RWDDLIKGAISFDNTELDDLIIARPDGTPTYNFCVVVDDMDMGITHVLRGDDHVNNTP
                     RQINILRALGATLPQYGHVPMILGPDGQKLSKRHGAVNVMEYDKQGYLPEAMVNYLAR
                     LGWSHGDDELFTREQLVQWFDTDHLSKSASQWDPKKLNWVNAHYLRQMAEAELAERVA
                     PRIAARGGNPRAVDLPAALALLKDRAETLEQLAEGAMLFCAPFQPAPAELAEQHLTPA
                     AREALADFAARAQGADWTKEGIAALIKAVLADRGLKMPQLAIPLRVAATGRAQTPAVD
                     AVLALLGKQTVLARLAAI"
     gene            3072..3716
                     /locus_tag="VGC69_09150"
     CDS             3072..3716
                     /locus_tag="VGC69_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY0295482.1"
                     /translation="MVPFHTKDRIIKTMHFAAAAAAAACLAGWGTAAQAGAGDILIRA
                     RAVSVTPQVSTGDTLSTLNAGVKQSVIPELDFTYMFTDHLGAELILGTTRNRLTSSLG
                     SLGKVSLLPPTATLQYHFNDEGAIRPYVGAGINYTRFYDNGLSAGGVPIGIKQNSFGP
                     ALQLGVDVKLSENWFFNVDVKKLWVRTDASLAGANLGTLKIDPWLFGVGVGYRF"
     gene            complement(3717..4901)
                     /locus_tag="VGC69_09155"
     CDS             complement(3717..4901)
                     /locus_tag="VGC69_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295483.1"
                     /translation="MTSGNRAILLLTLAAFASSSAFRLCDPMLPRLADEFGASTGEAA
                     HAVTAFAVGYGVLQMFFGPIGDRYGKYRVVCLATCLCALGSLGAVLSSSLDMLVASRF
                     LSGATGAGIVPMAMAWIGDHVSYERRQATLARFLTGTVMGMAFGQMAGGWFADALGWR
                     WAFGAMLVIYLVVGALLFFEVRRQSALGEGKGLGAGSAGLLAQGRQVLGVPWARVVLA
                     TVFLEGLCVFGALAFVPAYLHDRFDLSLTHAGGVMALYAAGGFAYTLVAGRVIKALGE
                     RGLAVSGGFVLCLAYLCYLLGPSWYWSVPASLMAGFGYYLLHATLQTNATQMVPAARG
                     TAVAWFASCLFLGQAVGVALAGSVVDVTGAPVLFAVSAAALLLLGLFFSQALHRRAAI
                     PA"
     gene            complement(4934..5443)
                     /locus_tag="VGC69_09160"
     CDS             complement(4934..5443)
                     /locus_tag="VGC69_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295484.1"
                     /translation="MRRSLFLPFAGTACAVLVLAGCVSRKPPAPVVEAPPPGPPPVVC
                     TPAPPDDPMVGTWYSVSRQKGVAGDYRTLTVLSPDGRMTYETQLKVGRKIRPALRESG
                     CWQVADGIYTMRTTLSNGEAVDTDDPIYTNRYRIEKFDRAKLTLRALKDGGQAVTAQR
                     MPPGYQLPF"
     gene            complement(5622..6074)
                     /locus_tag="VGC69_09165"
     CDS             complement(5622..6074)
                     /locus_tag="VGC69_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0295485.1"
                     /translation="MDKTDRAILSALQQDGRASAQQLSDQVGLSTAPVWRRVKALEAD
                     GVIQGYHAHIDRSKVGLQSCMFVQISLERHVASTVENFERSVRDAPEILECYAVTGDS
                     DFLLKILVESPEAYDKFLHRFLFNLPGIRQTRTIVALREIKHEVRLPL"
     gene            6258..9749
                     /locus_tag="VGC69_09170"
     CDS             6258..9749
                     /locus_tag="VGC69_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY0295486.1"
                     /translation="MDGTPAAFPGLDLDYRLDDNFTRTQGRVFLTGTQALVRALLTQA
                     RLDRERGLDTAGFASGYRGSPLAGVDLAMWKNKALLEAARIRFLPGINEDLAATAVMG
                     TQQAGLRQDRKVDGVFAMWYGKGPGLDRAGDALHHGNAAGASRHGGVLMVVGDDHTAV
                     SSSIPHASESSLIGWLVPIVNPASVAEYETFALWGWALSRHAGAWVAFKAISETVESG
                     QSFDLAPTPAYDMPDDPMLPRESIEYNSRDFLTPAIETRMLRRLEAVRAFAQRHSIDR
                     LAWPAPAASAGIIAVGKAYLDTLEALQRLGVAPDAVRLYKPGLTWPLDAGRLLDFARG
                     LRHILVIEEKGAVVESQVKDLLFNLPQHPSVCGRRGLDGEPLIPAAGQLRPSLLAAPL
                     SGWLDKAAGIAAAIGGAFQCPAPLSNEADGMRRRPYFCSGCPHSTSTKVPEGSQALAG
                     VGCHYMAAWMDRNTGGLTQMGGEGVDWVGLAPYVDMPHVFQNMGEGTYYHSGYLAIRQ
                     AIAARANITYKILFNDAVAMTGGQPVDGPISVPQICQQLRGENVARIVVTSDEPEKYR
                     GVDLGPGIAVHHRRELDALQRELREIRGVTVLIHDQTCAAEKRRRRKKNQFPDPPRRM
                     LINSAVCEGCGDCGVQSNCLSVTPLETPYGRKRAIDQSSCNKDYSCAEGFCPSFVSVL
                     NGKPRRQAAAPDGRGQLDARIAALPEPTLAPLAAPYRLMVAGVGGTGVITIGAIVSMA
                     AHLQGLSASTLDLTGLAQKGGTVLSHIRLAPAGADAGPVRLDWQQADAAILCDPVVSI
                     APDALGALRLGHTRVTLNTYVAPVSDFTRNPDAPLRAEELLAKIRHAAGDAQVAALDA
                     HAAAQSLFGDSILSNMFLLGHAWQRGAIPLSLQALARAIELNGVAVQANRAAFDAGRL
                     AAHDPQALGPLLAPRAQVVQLHMPESFDKAVARREHDLVAYQNAAYARQYRELVDRVA
                     DRERALLPGNRAPRLALAVARSLFKLMAYKDEYEVARLYTDGAFRAELASQFEGEMQL
                     RFHMAPPLFARKDPRTGVPRKIALGAWAEPALRVLRHGKGLRGTWFDPFGHTAERRME
                     RALIREYRQTVQALLDGLAPERLDLAVRIAALAETVRGYGHIKTAAAQKYRTELQGLM
                     QVYSRPSEEAIPLQKTA"
     gene            10036..>11511
                     /locus_tag="VGC69_09175"
     CDS             10036..>11511
                     /locus_tag="VGC69_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEY0295487.1"
                     /translation="MQTTIASVTRPSAVPPTTNAPADASSGQWAGYQIIRRNGSVVGF
                     EPSKIAIAMTKAFLAVNGGQGAASARVRELVEQLTSQVVTALVRNHPGGGTFHIENVQ
                     DQVELALMRSGQHDVARAYVLYREKRTQERAAAAHVLGGETAAPADEPAIQVTENGAR
                     RPLDMAALRATIEAAGVGLTQFVDTEAILKETLKNLYDGVPVDEVYKSAILSARALVE
                     KDPAYTQVTARLLLHTVRKEVLGEEVSQAGMETRYAEYFPTFITRGIEGGLLSPELAN
                     FDLARVAAALDARRDLQFDYLGLQTLYDRYFLHIRGTRIELPQVFFMRVAMGLALREA
                     DREARAIEFYEILSSFDFMSSTPTLFNAGTLHSQLSSCYLTTVSDDLEGIYEAIKENA
                     LLAKYAGGLGNDWTPVRALRSHIKGTNGESQGVVPFLKVVNDTAVAVNQGGKRKGAVC
                     TYLETWHLDIEEFLELRKNTGDERRRTHDMNTANWIPDLFMR"
BASE COUNT         1960 a         4233 c         3765 g         1553 t
ORIGIN      
        1 tcggccgaca gcggcacctg gaaagcgtcc agcagcgcct gcgcctcggc cgtgtcgagc
       61 tgccgccggc cggccgcgcg ggcgccagcg atcacggcgc gggcgccggc aatgtcgggc
      121 ggcagctcgg tcggctcgga aggctgggtc tgctgcagca gctgctggtt gtagttgtag
      181 gaggccagca cgccgaaggc atccaccgcc gactcgggcg tgcgaaaggc cggcgtgccg
      241 gcgtcgtcga gcatgcgccg caagggccgc attcccgcgt cgcccatgaa gcagctgatc
      301 accggcttgc gggccttggg ggcgatctgc gacaggcggc ccgccacggc gggcatgtcg
      361 gccaaggcat ccggggccag cagcaccagc acgccgtcga cgccgacatc ggccagcagg
      421 ttgtcgagca ggccctggat gcgatcgggc gtcagcggca catagctgat gacgggattg
      481 ttcgtggccg catcgggttc gagcatgtcc tgcaatgcgc ggcgcgtgcc gggcgcgagc
      541 tcggcgcgca aggtggggcc gtccggcccg atgagatcca tggccagctg cggcggcccg
      601 ttgccgttgg acagcacggc cacgcgccgg ccgcgcgggc gccgcacgtg gcccagcacc
      661 ttgaccgccg agaacagctg cacgaaatag cgcacgcgca cggcgccggc gcggcgcagg
      721 acggcgtcga acacggcatc gccgccgtcg tcgccgcggc cggtcttgag cacgatgacg
      781 ggcttgacgc tggcggccgc gcgcaaggcg ctcatgaact cgcgcgcggg gccggcttct
      841 tcgaggtaca gcacgatgct gtcggtgcgc ggatcgctgg cgaggaagtc cagcgtctgc
      901 gtcaggccca ccacggcctc gtcgcccagc gacaccaccg tggagaaacc gatgtgcacg
      961 tcctcggccc agtccatgat ggcggcggcg atggagcgcg actgcgtcac cagcgccacc
     1021 cgcccggcca gcgccagcgt cggatgctgg ctgagattga gctggctgtg cggccgctgc
     1081 acgccgaaag aacgcgggcc gagcagcacg caatcgtgct gctcggccca ggcctcgcac
     1141 agggcgcgcg tgccgccggg ataggggtcg ggcagttcgt ggggcaggac gatgacgccg
     1201 cgcggcgcca ggccgccgag ccgccgcagg gtctcgggca gcacggcggg cgacacgcac
     1261 accagcgcca ggtcgggccg ctcgcccggg gccaggccct ggcaggtccc gggcggcacc
     1321 ggcgccaggc caggttcgca ttcgacgacc gtgacgcgcg cgcgcaggcc ggccggcagc
     1381 gcggccagga ccggcaggtc gcggtcggcc acgaccacca gggagcctgg ctcgaacagg
     1441 ggcgctaggg cgtgtcgcag catgatggcg cgggcggcag tcgggcactc atctaaaata
     1501 ggaggatcat cgtaacaaga ccgcagcccg acatccacgg ttgcgtgcct ttccgccgcg
     1561 gccccacgcg ccgcctctca ccgcttcacc atgaccgcac aaaatccctc cgtcgtccgc
     1621 acccgcttcg cgccctcgcc caccggcttc ctgcacctgg gcggcgcgcg caccgcgctg
     1681 ttctcctggg ccttcgcccg ccatcaccag ggcaccttcg tgctgcgcat cgaggacacc
     1741 gacgtggaac gctccaccca ggcggccgtg gacgccatcc tcgacagcat ggcctggctg
     1801 ggcatgcagc ccgacgaagg cccgttctat cagatgcagc gcatggaccg ctaccgcgaa
     1861 gtgctggccg gcatgctgca agaaggcacg gcctaccact gctacagttc gcccgaagaa
     1921 gtcgaggcca tgcgcgaggc cgcccgcgcc aagggactga agccgcgcta cgacggcacc
     1981 tggcgccccg agccgggcaa ggcgctgccg cccatccccg caggccgcaa gccggtggtc
     2041 cgcttcaaga acccgcaaga cggcgccacc cgttgggacg acctgatcaa gggcgccatc
     2101 agcttcgaca acaccgagct cgacgacctc atcatcgcgc gccccgacgg cacgcccacc
     2161 tacaacttct gcgtggtggt cgacgacatg gacatgggca tcacccatgt gctgcgcggc
     2221 gacgaccacg tcaacaacac gccccgccag atcaacatcc tgcgcgcgct gggcgccacg
     2281 ctgccgcaat acggccacgt gcccatgatc ctgggccccg acggccagaa gctctcgaag
     2341 cggcacggcg cggtcaacgt catggaatac gacaagcaag gctacctgcc cgaggccatg
     2401 gtcaactacc tggcccgcct gggctggagc cacggcgacg acgagctctt cacccgcgag
     2461 cagctggtgc aatggttcga caccgaccac ctgtccaagt ccgcctcgca gtgggacccg
     2521 aagaagctca actgggtcaa tgcccactac ctgcgccaga tggccgaggc cgaactggcc
     2581 gaacgcgtcg cgccgcgcat cgccgcgcgc ggcggcaacc cgagggccgt ggacctgccc
     2641 gccgccttgg ccctgctcaa ggaccgcgcc gagaccctgg aacaactggc cgaaggcgcc
     2701 atgctcttct gcgccccctt ccagcccgcg cccgccgaac tcgccgagca gcacctgacg
     2761 ccggccgcgc gcgaagccct ggccgacttc gcggcccgcg cgcaaggcgc cgactggacc
     2821 aaggaaggca tcgccgccct cataaaggcc gtgctggccg accgcggcct gaagatgccg
     2881 caactggcca tcccgctgcg cgtggccgcg accggccggg cgcagacgcc ggcggtcgat
     2941 gcggtattgg ccctgctggg caagcaaacc gtgctggcgc gcctggccgc gatctgaagc
     3001 ggcacacgcc gcgccgcttt cgcaggcgga tttgatctag cgcaaatggc cggcgggaga
     3061 tgggcgcgat catggtgccg ttccacacca aggaccgcat cataaaaacc atgcatttcg
     3121 ccgccgcggc cgcggcggcc gcctgcctgg ccggctgggg cacggccgcc caggccggcg
     3181 ccggcgacat cctcatccgg gcccgcgccg tgagcgtcac cccgcaggtc tccaccggcg
     3241 acaccctgtc caccctgaac gcgggcgtca agcagtcggt catccccgag ctggacttca
     3301 cctatatgtt caccgaccac ctcggcgccg agctgatcct gggcacgacg cgcaaccgcc
     3361 tcacctccag cctgggcagc ctgggcaaag tgagcctgct gccgccgacg gccacgctgc
     3421 aataccactt caacgatgaa ggcgccatcc gcccctacgt gggcgccggc atcaactaca
     3481 cgcgcttcta cgacaacggc ctgtcggccg gcggcgtgcc catcggcatc aagcagaaca
     3541 gcttcggccc ggccctgcag ctgggcgtgg acgtgaagct gagcgaaaac tggttcttca
     3601 acgtcgacgt gaagaagctg tgggtgcgca ccgacgcctc gctggccggc gccaacctgg
     3661 gcacgctgaa aatcgacccc tggctgttcg gcgtgggcgt gggctaccgc ttctgatcag
     3721 gcggggatgg cggcgcgccg atgcaaagcc tgcgagaaaa acaggcccag cagcagcagc
     3781 gcggcggccg acacggcgaa cagcaccggc gcgccagtca catccaccac cgagccggcc
     3841 aaggccacgc ccaccgcctg ccccaggaac aggcacgagg cgaaccacgc caccgccgtg
     3901 ccgcgcgcgg ccggcaccat ctgcgtggcg ttggtctgca gcgtggcgtg caggaggtaa
     3961 tagccgaacc ccgccatcag gctggccggc acgctccagt accaggacgg tcccagcaaa
     4021 tagcacagat aggccaggca aaggacgaac cctccggaca ccgccaggcc gcgctcgccc
     4081 agcgccttga tcacgcgccc ggccaccagg gtgtaggcga acccgcccgc ggcatacagc
     4141 gccatcaccc ctccggcatg cgtgagcgag aggtcgaaac gatcgtgcag ataggccggc
     4201 acgaaagcca gcgcgccgaa tacgcacaag ccttccagga acaccgtggc cagcaccacg
     4261 cgcgcccacg gcacgcccag cacctggcgg ccctgcgcca acagcccggc gctgccggct
     4321 cccaggccct tgccctctcc gagcgcggat tggcggcgca cctcgaaaaa cagcagcgcg
     4381 ccgaccacca ggtaaatgac cagcatggcg ccaaaggccc agcgccagcc cagggcatcg
     4441 gcaaaccagc cgccagccat ctggccgaac gccatgccca tcaccgtgcc ggtgagaaac
     4501 cgcgccaggg tcgcctgccg gcgctcataa gacacgtggt cgcctatcca ggccatggcc
     4561 atgggcacga tgcccgcgcc agtggcgccc gacaggaagc ggctggccac cagcatgtcc
     4621 agcgaactcg acagcaccgc ccccaggctg cccagggcgc acaggcaggt ggccaggcac
     4681 accacgcggt acttgccgta gcggtcgccg atggggccga agaacatctg cagcaccccg
     4741 tagcccacgg caaaggccgt gacggcatgg gccgcctcgc ccgtgctggc gccgaactcg
     4801 tcggccagcc tgggcagcat ggggtcgcag aggcgaaagg cgctggaact ggcaaaggcc
     4861 gccagggtca gcagcaaaat ggctcggttt ccggaagtca ttgctgcatg ggcctcgtcg
     4921 gcaagcgatg cggttagaag ggcaactggt agccgggcgg catgcgctgc gcggtgaccg
     4981 cctggccgcc gtccttgagc gcgcgcaagg tcagcttggc gcggtcgaat ttttcgatgc
     5041 ggtagcggtt ggtgtagatc ggatcgtcgg tatcgaccgc ttcaccgttg gaaagtgtag
     5101 tccgcatggt gtagatgccg tcggcgacct gccagcaacc cgactcgcgc agggccgggc
     5161 ggatcttgcg gccgaccttg agctgtgttt cgtaggtcat gcggccgtcg ggcgacagga
     5221 ccgtcagcgt ccggtagtcg cccgccacgc ccttttgcct ggacaccgag taccaggtgc
     5281 cgaccatggg gtcgtccggc ggggcgggcg tgcagacgac agggggagga ccaggcggcg
     5341 gcgcctcgac taccggcgca gggggcttgc ggctgacgca gccggccagg acgagcacgg
     5401 cacaggctgt gccggcaaaa ggcaaaaaca acgaacgacg catgattcca ggggccaagc
     5461 ccgcacaaga gcggccgcat cacgcgatgc ggctgtcaaa cactgctctc actctaaaga
     5521 ccggaaaccc tcaaatcaat gaagtcgcgc gcactttgcc ccgctttgcg ggcggttggt
     5581 gcaatcgcgc aacattcaag aacgcgccgg gccgggcgcc gctacagggg cagccggacc
     5641 tcgtgcttga tctcgcgcag ggccacgatg gtgcgggtct ggcggatgcc gggcaggttg
     5701 aacaggaagc gatgcaggaa cttgtcgtag gcctccgggc tttcgaccag gatcttcagc
     5761 aggaagtccg agtcgcccgt gacggcgtag cactcgagaa tctcgggcgc gtcgcgcacc
     5821 gagcgttcga agttctcgac cgtgctggcg acgtgccgct ccaggctgat ctgcacgaac
     5881 atgcagctct gcaggcccac cttgctgcgg tcgatgtgcg cgtgatagcc ctggatgacg
     5941 ccgtcggcct ccagcgcctt gacccgccgc cacaccggcg cggtcgacaa gccgacctgg
     6001 tcggacaact gctgggccga agcccggccg tcttgctgca gggcgctcag gatggcgcgg
     6061 tcggtcttgt ccatggcggg cttcctccgg gcggcgggca aaaaatacgc aattgataaa
     6121 ttcacatgcc cggaatcggg caaaacgaaa cgtttgtttc gcattatagg gaattcacga
     6181 tccactaacg caataaaagg ccaggccgct ctgtctatca tggctcaaaa caagcattcc
     6241 attctggaga caatgccatg gacggcaccc ccgccgcctt tccgggcctg gacctcgatt
     6301 accggctcga cgacaacttc acccgcaccc agggccgcgt cttcctcacg ggcacgcagg
     6361 cgctggtgcg cgccctgctg acccaggccc ggctggaccg cgaacgcggc ctggacaccg
     6421 cgggcttcgc ctcgggctac cggggctcgc cgctggccgg ggtggacctg gccatgtgga
     6481 agaacaaggc gctgctggaa gccgcccgca tccgcttcct gcccggcatc aacgaagacc
     6541 tggccgccac cgccgtcatg ggcacccagc aggccggcct gcgccaggac cgcaaggtcg
     6601 atggcgtatt cgccatgtgg tacggcaagg gcccggggct ggatcgcgcc ggcgacgccc
     6661 tgcaccacgg caacgccgcc ggcgcctcgc gccacggcgg cgtgctgatg gtggtgggcg
     6721 acgaccacac cgccgtgtcc tcgtcgatcc cgcatgccag cgaatcctcg ctgatcggct
     6781 ggctggtacc catcgtcaat ccggcctcgg tcgccgagta cgagaccttc gcgctgtggg
     6841 gctgggccct gtcgcgccat gcgggcgcct gggtggcctt caaggcgatt tcggaaaccg
     6901 tggaaagcgg ccagtccttc gacctggcgc ccacgccggc ctacgacatg ccggacgacc
     6961 ccatgctgcc gcgcgagtcc atcgagtaca actcgcgcga cttcctcacc ccggccatcg
     7021 agacgcgcat gctgcggcgc ctggaagcgg tgcgggcctt cgcgcaacgc cacagcatcg
     7081 accggctggc ctggcccgcg ccggccgcct cggccggcat catcgccgtg ggcaaggcct
     7141 acctggacac cctggaggcc ttgcagcgcc tgggcgtggc cccggacgcc gtgcgcctct
     7201 acaaacccgg cctgacctgg ccgctggacg ccgggcgcct gctcgacttc gcccggggcc
     7261 tgcgccacat cctggtgatc gaggaaaaag gcgcggtggt cgagagccag gtcaaggacc
     7321 tgctcttcaa cctgccgcag cacccgtcgg tgtgcggccg gcgcggcctg gacggcgagc
     7381 cgctgatccc cgcggccggc cagttgcggc cctcgctgct ggccgcgccg ctgtccggct
     7441 ggctggacaa ggcggccggc atcgcggccg cgatcggcgg cgccttccag tgcccggcgc
     7501 cgctgtccaa cgaagccgac ggcatgcgcc gccggcccta cttctgctcg ggctgcccgc
     7561 acagcacctc gaccaaggtg cccgagggca gccaggcgtt ggccggcgtg ggctgccact
     7621 acatggcggc ctggatggac cgcaacaccg gcggcctgac gcagatgggc ggcgagggcg
     7681 tggactgggt gggcctggcg ccctacgtcg acatgcccca cgtgttccag aacatgggcg
     7741 agggcactta ttaccactcg ggctacctgg ccatccgcca ggccatcgcc gcgcgcgcca
     7801 acatcaccta caagatcctc ttcaacgacg ccgtggccat gaccggcggc cagccggtgg
     7861 acggtccgat ctcggtgccg cagatctgcc agcaattgcg cggcgagaac gtggcgcgca
     7921 tcgtggtcac gtccgacgag cccgagaaat accgcggcgt ggacctcggc cccggcatcg
     7981 ccgtgcacca ccgccgcgag ctcgacgccc tgcagcgcga attgcgcgag atacgcggcg
     8041 tgaccgtgct gatccacgac cagacctgcg ccgccgagaa gcgccgccgc cgcaagaaga
     8101 accagttccc cgacccgccg cgccgcatgc tgatcaacag cgccgtgtgc gagggctgcg
     8161 gcgactgcgg cgtgcagtcc aactgcctgt cggtcacgcc gctggagacg ccctacggcc
     8221 gcaagcgcgc catcgaccag tcgagctgca acaaggacta ctcctgcgcc gagggcttct
     8281 gccccagttt cgtctcggtg ctgaacggca agccgcgccg gcaggccgcc gcgccggacg
     8341 gccgcggaca gctcgacgcg cgcatcgccg ccctgcccga gcccacgctc gccccgctgg
     8401 ccgcgcccta ccggctgatg gtggccggcg tcggcggcac cggcgtcatc accatcggcg
     8461 ccatcgtctc gatggccgcc cacctgcagg gcctgtcggc ctcgacgctg gacctcaccg
     8521 gcctggcgca aaagggcggc acggtgctca gccacatccg cctggcgccg gcgggcgccg
     8581 acgccggccc ggtgcgcctg gactggcagc aggccgacgc cgccatcctc tgcgatccgg
     8641 tggtgtcgat cgcccccgac gcgctgggcg ccctgcgcct gggccacacg cgcgtcacgc
     8701 tcaacaccta cgtggccccg gtctcggact tcacccgcaa ccccgacgcg ccgctgcgcg
     8761 ccgaggaact gctggccaag atccgccacg cggccggcga cgcgcaggtc gcggccctgg
     8821 acgcccacgc cgccgcgcag tcgctattcg gcgacagcat cctgtcgaat atgttcctgc
     8881 tgggccatgc ctggcagcgc ggcgccattc cgctctcgct gcaggccctg gcgcgcgcca
     8941 tcgagctcaa cggcgtggcg gtgcaggcca accgcgcggc cttcgacgcc ggccggctgg
     9001 ccgcgcacga cccgcaggcg ctcggccccc tgctcgcgcc gcgcgcccag gtggtgcagt
     9061 tgcatatgcc ggaatccttc gacaaggcgg tggcgcggcg cgaacatgac ctggtcgcct
     9121 accagaacgc ggcctacgcg cggcaatacc gcgagctggt cgaccgcgtg gccgaccgcg
     9181 agcgcgcgct gctgcccggc aatcgcgcgc cgcgcctggc cctggccgtg gcccgcagcc
     9241 tcttcaagct gatggcctac aaggacgagt acgaagtggc gcggctctat accgacggcg
     9301 ccttccgcgc cgagctggca agccagttcg agggcgaaat gcagctgcgc tttcacatgg
     9361 cgccgccgct cttcgcccgc aaggacccgc gcaccggcgt gccccgcaag atcgcgctgg
     9421 gcgcctgggc cgaaccggcc ctgcgcgtgc tgcgccacgg caagggcctg cgcggcacct
     9481 ggttcgaccc cttcggccac accgccgaac gccgcatgga gcgcgcgctg atccgggaat
     9541 accggcaaac ggtgcaggcc ctgctcgacg gcctggcccc cgaacggctg gatctggccg
     9601 tgcggatcgc cgccctggcc gagaccgtgc gcggctacgg ccacatcaag accgccgccg
     9661 cgcagaaata tcggaccgag ctccagggac tgatgcaggt ctattcacgg ccctccgaag
     9721 aggcaattcc cctgcagaag accgcctgac gcggatgcga agtgaacact tcggcggtcg
     9781 cgccctaccc ggcgcgaccg aaggccgcgc ctatctttac gattaacaat tactaacatc
     9841 aagcttaccg acacatgctt gtcgtatgcc gtctccctca ctagaattag tagcaacaga
     9901 ggggtgatac actacatata gtaggtcaca tccgccgcgc caccagcacg gcctgcccgc
     9961 aacatccccc tctcacattc ccaggcaagg ctgccccggc cttgcctttc gcgccttttc
    10021 cgcacaggag cttccatgca gactacgatc gcctctgtga ctcggccctc ggccgtgccg
    10081 cccaccacca acgccccggc cgacgcttcc agcggccagt gggcgggtta ccagatcatc
    10141 cgccgcaacg gctcggtggt cggctttgaa cccagcaaga tcgccatcgc catgaccaag
    10201 gccttcctgg ccgtcaacgg cggccagggc gcggcctcgg cgcgcgtgcg cgagctggtc
    10261 gaacaactca ccagccaggt ggtcaccgcg ctggtgcgca accaccccgg cggcggcacc
    10321 ttccacatcg aaaacgtgca ggatcaggtc gaactcgccc tgatgcgctc cggccagcac
    10381 gacgtggccc gcgcctacgt gctctatcgt gaaaagcgca cccaggagcg cgccgcggcc
    10441 gcccatgtgc tgggcggcga aaccgccgcg cccgccgacg aacccgccat ccaggtcacc
    10501 gagaacggcg cgcgccgccc gctcgacatg gccgccctgc gcgccaccat cgaagcggca
    10561 ggcgtcggcc tgacccagtt cgtcgacacc gaggccatcc tcaaggaaac cctcaagaac
    10621 ctgtacgacg gcgtgccggt cgacgaagtc tacaagtcgg ccatcctgtc cgcccgcgcg
    10681 ctggtcgaga aagacccggc ctacacccag gtcaccgccc gcctgctgct gcacacggtg
    10741 cgcaaggaag tgctgggcga ggaagtctcg caggccggca tggaaacccg ctacgccgaa
    10801 tacttcccca ccttcatcac gcgcggcatc gaaggcggcc tgctgtcgcc cgaactggcc
    10861 aatttcgacc tggcccgcgt ggccgccgcg ctcgacgccc gccgcgacct gcagttcgac
    10921 tacctcggcc tgcagaccct gtacgaccgc tacttcctgc acatccgcgg cacccgcatc
    10981 gaactgccgc aggtgttctt catgcgcgtg gccatgggcc tggcgctgcg cgaagccgac
    11041 cgcgaagccc gcgccatcga gttctacgag atcctgtcgt ccttcgactt catgagctcg
    11101 acgcccacgc tgttcaacgc gggcacgctg cactcgcagc tgtcgtcgtg ctacctcacc
    11161 accgtgtccg acgacctgga aggcatctac gaagccatca aggaaaacgc cctgctggcc
    11221 aagtatgccg gcggcctggg caacgactgg acgccggtgc gcgcgctgcg cagccacatc
    11281 aagggcacca acggcgaaag ccagggcgtc gtgcccttcc tgaaggtggt caacgacacc
    11341 gccgtggcgg tgaaccaggg cggcaagcgc aagggcgccg tttgcaccta cctggaaacg
    11401 tggcacctcg acatcgaaga gttcctcgag ctgcgcaaga acaccggcga cgagcgccgc
    11461 cgcacgcacg acatgaacac ggcgaactgg attcccgacc tgttcatgcg c
//
LOCUS       DASWPO010000168         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2358569, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000168 DASWPO010000000
VERSION     DASWPO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358569"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..777
                     /locus_tag="VGC69_09180"
     CDS             55..777
                     /locus_tag="VGC69_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295488.1"
                     /translation="MLSIKNLEAGYGKVKVLHGISMEVPKAKVVTLIGSNGAGKTTTM
                     RALSGMIAPSAGEIMLGGKRIDGLASHRIARLGLAHSPEGRRVFPTLSVIDNLLLGAF
                     PRLTGRRPKGDVQADLERALTLFTRLKERRNQLAGTLSGGEQQMLAMARAVMLNPDVV
                     LLDEPSMGLAPILVEEVFRIIERLKEQGVTMLLVEQFAVAALNVADYGYVLENGRISV
                     HGTPDKLRHDPAVVAAYLGGAH"
     gene            complement(794..1705)
                     /locus_tag="VGC69_09185"
     CDS             complement(794..1705)
                     /locus_tag="VGC69_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HEY0295489.1"
                     /translation="MEHLSRIDHDQLRALAAVVREGSFERAAARLSVTPSAVSQRIKL
                     LEHRIGRLLVRRTTPASATADGEVLVRLAEQAALLEHEAFVRLGVDDAAPRAGIRVAV
                     NHDSLETWFVDAAARFAAQTDITLDMQTEDQDHTAALLRDGSVLGAVTTLADPVQGCR
                     IHALGSMRYVATCTPAFHALHFSQGVGARTLAAAPVLVFNRKDAMQARFVQRVMRGAA
                     WQPPVWWLPSSRAFVQATLAGLGWTMNMQILVQEHLDSGRLVPLRPRAWEDVPLYWQH
                     WRVETQAMHQLTDAVLAAAAGQLRRGR"
     gene            1806..2438
                     /locus_tag="VGC69_09190"
     CDS             1806..2438
                     /locus_tag="VGC69_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY0295490.1"
                     /translation="MLFSLPFSALFGAWFSGAATGLGLFAAVGAQSAYILRQGILRAH
                     LLGILAVCALLDATFIFASVLGLRMLDHGLPWLTQAISGFGVAFLAWYALQAARRALR
                     PGQGLQAAAQGRHSRRAALLGAVSLSLFNPHFWLDIMVVGSLAHGLDQDRLAFGAGAM
                     TASLFWLAVLGLGSRLFAPLFAAPRAWRVLDGGMAVLMSALALKMAAAAI"
     gene            complement(2467..>2737)
                     /locus_tag="VGC69_09195"
     CDS             complement(2467..>2737)
                     /locus_tag="VGC69_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEY0295491.1"
                     /translation="RLAGRGGGVFLRLLLTPELWVASQIRKRATTMAWTPGLTLDYAR
                     AMSMLADPRPSGWWKMSRAAIRRMAVLRTGWRRQDRQAAQQREDL"
BASE COUNT          411 a          989 c          923 g          414 t
ORIGIN      
        1 gacggcaaag tgatcgaagc ctatctgggc ggcgcgaccg cataggagaa aaagatgcta
       61 tcgatcaaga atctcgaagc gggctacggc aaggtcaagg ttctgcacgg catcagcatg
      121 gaggtgccca aggccaaggt cgtgacgctc atcggctcca acggcgcggg caagaccacc
      181 accatgcgcg cgctctcggg catgatcgcg cccagcgccg gcgaaatcat gctgggcggc
      241 aagcgcatcg acggcctggc gtcgcaccgc atcgcgcggc tgggcctggc ccactcgccg
      301 gaaggccggc gcgtctttcc cacgctgtcg gtcatcgaca acctgctgct gggcgccttt
      361 ccgcgcctga cgggccgccg cccgaagggc gacgtgcagg ccgacctgga acgcgccctg
      421 acgctcttca cccgcctgaa ggagcgccgc aaccagctgg ccggcacctt gtccggcggc
      481 gaacagcaga tgctggccat ggcgcgcgcc gtgatgctca accccgacgt ggtgctgctc
      541 gacgaaccgt ccatgggcct ggcgcccatc ctggtcgaag aggtctttcg catcatcgag
      601 cgcctgaagg aacagggcgt gacgatgctg ctggtggagc agttcgccgt cgcggcgctc
      661 aacgtggccg actacggcta tgtgctggag aacggccgca tctcggtgca cggcacgccg
      721 gacaagctgc gccacgaccc ggcggtggtg gcggcctacc tgggcggggc gcactgaacc
      781 gcgcgagccg gcatcaacgc ccgcgccgca actgcccagc ggcggctgcc agcacggcgt
      841 cggtgagctg gtgcatggcc tgcgtctcca cccgccagtg ctgccagtaa agcggcacgt
      901 cttcccaggc gcgcgggcgc agcggcacca ggcgtcccga atccaggtgc tcctgcacca
      961 ggatctgcat gttcatcgtc cagcccaacc cggccaaagt ggcctgcacg aaagcccgtg
     1021 acgaaggcag ccaccacacc ggcggctgcc aggcagcgcc gcgcatcacg cgctggacaa
     1081 agcgggcctg catcgcgtcc ttgcggttga acaccagcac cggcgccgcg gccagcgtgc
     1141 gggcgccgac gccctgcgag aaatgaagcg cgtggaatgc cggcgtgcag gtggccacat
     1201 agcgcatgct gcccagggca tggatgcggc agccctgcac cggatcggcc agggtggtca
     1261 ccgcgcccag gaccgaaccg tcgcgcagca gggcggcggt gtggtcctgg tcctcggtct
     1321 gcatgtccag cgtgatgtcg gtctgcgcgg caaaacgcgc cgccgcgtcg acgaaccagg
     1381 tttccaggct gtcgtggttg accgccacgc ggatgccggc ccgcggcgcc gcgtcgtcca
     1441 cccccagccg cacgaaagcc tcgtgctcga gcagggccgc ctgctcggcc aggcgcacca
     1501 gcacctcgcc gtcggccgtg gcgctggccg gcgtggtgcg ccgcaccagc aggcggccga
     1561 tgcggtgctc cagcagcttg atgcgctgcg acaccgccga gggcgtcacg ctgagccgcg
     1621 cggcggcccg ctcgaagctg ccttcgcgca ctaccgccgc cagggcgcgc aactggtcgt
     1681 gatcgatgcg ggaaagatgt tccatgacat tagaaattct taagtgattt aaggaaaatt
     1741 agctggaatc catgccgacg gcaagccaga atgccaaccg catcacgctt tcccctggat
     1801 tcaccatgct gttttccctg cctttctccg ccctgttcgg cgcctggttc agcggcgccg
     1861 ccaccggcct gggcctgttc gccgccgtgg gcgcgcaaag cgcctacatc ctgcgccagg
     1921 gcatcctgcg cgcccacctg ctcggcatcc tggccgtctg cgcgctgctc gacgcgacgt
     1981 tcatcttcgc cagcgtgctg ggactacgga tgctggacca tggcctgccc tggctgacgc
     2041 aggccatatc gggattcggc gtcgccttcc tggcctggta tgcgctgcag gccgcgcgcc
     2101 gcgcgctgcg gccaggccag ggcctgcagg ccgcggcgca aggccgccac tcgcgccgcg
     2161 cggcgctgct gggcgcggtg agcctatcgc tgttcaatcc gcacttctgg ctggacatca
     2221 tggtggtggg ctcgctggcg cacggcctcg accaggaccg cctggccttc ggcgccggcg
     2281 ccatgaccgc gagcctgttc tggctggccg tcctaggcct gggctcgcgc ctgttcgcgc
     2341 cgctgttcgc cgcgccgcgc gcctggcgcg tgctggacgg cggcatggcc gtgctgatgt
     2401 ccgcgctggc gctgaaaatg gccgccgccg cgatatagcc tcaacccgcg gcgtcttccc
     2461 ggcgcctcag aggtcttccc gctgctgcgc ggcttgccgg tcctggcgcc gccagccggt
     2521 gcgcagcacg gccatccgcc ggatcgcggc ccgcgacatt ttccaccagc cggacggccg
     2581 cggatcggcc agcatgctca tggcccttgc gtaatccagc gtcagtccag gcgtccaggc
     2641 catcgtcgtg gcgcgcttgc ggatctgcga ggcgacccac agctcgggcg tcaacagcag
     2701 gcgcaggaat accccgccgc cccggccggc cagccgc
//
LOCUS       DASWPO010000169         5760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2359514, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000169 DASWPO010000000
VERSION     DASWPO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359514"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VGC69_09200"
     CDS             <1..1366
                     /locus_tag="VGC69_09200"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY0295492.1"
                     /translation="AAYLGYEAQVASGEITLFKKMPAIALWRKMLSMLFETGHPWITF
                     KDPCNIRSPQQHVGMVHSSNLCTEITLNTNDSEIAVCNLGSVNLPRHMKPATNGGYEL
                     DHDKLKRTVGIAMRMLDNVIDINYYAVDKARNSNARHRPVGMGIMGFQDCLHMMRVPY
                     ASQAAVEFADRSMEAVCYQAYWASSGLAQERGQYQSYEGSLWSRGILPQDTIAMLREA
                     RGGHVEVDASSTLDWDALRARIKTQGMRNSNCIAIAPTATISNIIGVSACIEPTYQNL
                     YVKSNLSGEFTVVNDYLVRDLKKLGLWDEVMVADLKYFDGSLSRIDRVPAELRELYAT
                     AFEVEPSWLVECASRRQKWIDQSQSLNIYMAGASGKKLDDTYKLAWLRGLKTTYYLRT
                     LGATSAEKSTGRGGELNAVNSSGIANPAASAAAPVLPEPEIVGAACTMKPGDPGFEEC
                     EACQ"
     gene            1385..2566
                     /locus_tag="VGC69_09205"
                     /pseudo
     CDS             1385..2566
                     /locus_tag="VGC69_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
     gene            2899..3366
                     /locus_tag="VGC69_09210"
     CDS             2899..3366
                     /locus_tag="VGC69_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like DNA-binding protein"
                     /protein_id="HEY0295493.1"
                     /translation="MATAKKAAKKAVKKTPAKKAVKKAVKKVAAKKAVVKKAAVKKVA
                     VKKVAKKAVKKVAKKAVKKVAAKKPAAKKVAKKAAKKAVAKKAVKKVAKKAAKKTAKK
                     AAKKAAKPAAKKPAAKKAAPKKPATPPSTAAAPGAKTALNPAASWPFPTGGRP"
     gene            3500..4060
                     /locus_tag="VGC69_09215"
     CDS             3500..4060
                     /locus_tag="VGC69_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY0295494.1"
                     /translation="MIGDIARFLLDLCFTLFGAALILRAWMHAVRLPPFNPVARGVYQ
                     ASNWLILPLRKLIPATSRIDWTSLAALWLTALVYLLLAWLVSVGALIPPALLPSVAAS
                     ALLMAVKWALNLVVWMTLAQAVLSWVNPLSPLMPILQTLTAPLLGPIRQILPRMSVDF
                     SPLLLLVIAQVLLMVLTRVGYGLMGI"
     gene            complement(4175..4672)
                     /locus_tag="VGC69_09220"
     CDS             complement(4175..4672)
                     /locus_tag="VGC69_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY0295495.1"
                     /translation="MMQSNSSSFVRAGRWTAVAALGLSLSVLAGCADRSASSSVYTYG
                     QAQREQIVRTGTIVAMRPITIQNDRSSGVGLIAGGVLGGVAGNGIGRGSGNALATVGG
                     AILGAVAGNAVENQVGKTSGYEITVRLDNGETRVVAQEADQPLGVGQRVQVISGAGPT
                     RVAPM"
     gene            complement(4823..>5760)
                     /locus_tag="VGC69_09225"
     CDS             complement(4823..>5760)
                     /locus_tag="VGC69_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY0295496.1"
                     /translation="PVLVCGSMAFDAIAVFEGRFGEHILPDRIHTLSVSFLVPSLRRE
                     YGGCAGNVAYNLKLLGGAPLPVASVGEDAGGYLQRLTDLGISTAHVKVVPGTLTAQCW
                     ITTDLDDNQITAFHPGAMAMSAQNDFGSDTRAVWGVVSPDAKEGMFAHAERLNKLGIP
                     FIFDLGQAMPLFDGADIERMIRLAQVLTANDYEASVIEQRTGRQIADIAKTMQAVIVT
                     RGGEGATLMTGGETIEVPPVKAAAVVDPTGCGDSHRAGLVYGLTQGWNWLDSCRLASV
                     MGAIKIASRGAQNHKPARGEIDGLLHASYGIHLPA"
BASE COUNT         1109 a         1934 c         1774 g          943 t
ORIGIN      
        1 agcggcctac ctgggctacg aagcccaggt cgccagcggc gagatcacgc tcttcaagaa
       61 gatgccggcc atcgcgctgt ggcgcaagat gctgtcgatg ctgttcgaaa ccggccaccc
      121 ctggatcacg ttcaaggatc cttgcaacat ccgctcgccg cagcagcacg tgggcatggt
      181 gcacagctcc aacctttgca ccgagatcac gctcaacacc aacgactcgg aaatcgccgt
      241 ttgcaacctg ggctcggtca acctgccccg gcacatgaag cccgccacca acggcggcta
      301 cgaactcgac cacgacaagc tcaagcgcac cgtcggcatc gccatgcgca tgctcgacaa
      361 cgtgatcgac atcaactact acgccgtcga caaggcccgc aactccaacg cccgccaccg
      421 cccggtgggc atgggcatca tgggcttcca ggattgcctg cacatgatgc gcgtgccgta
      481 cgcgtcgcaa gccgcggtgg agttcgccga ccgctcgatg gaagccgtgt gctaccaggc
      541 ctactgggcc tcgagcggac tcgcgcaaga acgcggccaa taccagtcgt acgaaggttc
      601 gctgtggtcg cgcggcatcc tgccgcaaga cacgatcgcg atgctgcgcg aagcgcgcgg
      661 cggccatgtc gaggtcgatg catcgagcac gctcgattgg gatgcgttgc gcgcacgcat
      721 caaaacgcag ggcatgcgca actccaactg cattgcgatt gcaccaaccg cgacgatttc
      781 caatatcatt ggcgtgtctg cgtgcattga acctacatac caaaacttgt acgtcaaatc
      841 gaatctctct ggcgagttca cggtcgttaa cgattacctc gtgcgtgacc tgaagaaact
      901 cggtctgtgg gacgaagtca tggtcgccga tctcaagtac ttcgacggca gcctgtcccg
      961 catcgatcgc gttcccgccg aacttcgcga actctacgcc accgcgttcg aagtcgaacc
     1021 gagctggctg gtcgagtgcg cgtcgcgtcg acagaaatgg atcgaccagt cccagtcgct
     1081 gaacatctac atggccggcg cctcgggcaa gaagctcgac gacacctaca agctggcctg
     1141 gctgcgtggc ctgaagacca cctactacct gcgcacgctg ggcgccacca gcgccgagaa
     1201 gtccaccggc cgcggcggcg agctcaacgc cgtgaactcc tccgggattg ccaaccccgc
     1261 tgcctctgcc gcggcccctg tcctgcccga acccgaaatc gtcggcgcag cctgcaccat
     1321 gaaaccgggc gaccccggtt tcgaagagtg cgaagcctgc caataacgtt tcggagaatt
     1381 caatatgctg acatgggaag acaacgccgc ccaacaaccg gcacccgctg ccgggggttc
     1441 cacgggtggt cccgccgccg aggcacagcc cgccacgggg ctgttcgccg acacggccat
     1501 tccgccttcg gccgccgcac cgcagcaagc ggctggcgcc gaggcggcgc gccgcgtcaa
     1561 agtggccgac aagcgcatca tcaacggcca gaccgacgtc aaccagctgg tgccgttcaa
     1621 gtacaaatgg gcctgggaaa aatacctggc cacctgcgcc aaccactgga tgccgcagga
     1681 aatcaacatg tcgcgcgaca tcgcgctgtg gaagaacccc aacgggctga cggaagacga
     1741 gcgccgcgtc gtcaagcgca acctgggctt cttcgtcacc gccgactcgc tggccgccaa
     1801 caacatcgtg ctgggcacct accgccacat cacggcgccc gagtgccgcc agttcctgct
     1861 gcgccaggcc ttcgaagagg ccatccacac gcacgcctac cagtacatcg tggaaagcct
     1921 ggatctcgac gaatccgaga tcttcaacgc ctacaacgaa gtgccggcga tccgcgccaa
     1981 ggatgagttc ctcatcccct tcatcgacgc catctccgac cccaacttca ataccggcac
     2041 ccccgaggcc gaccagacgc tcctgaagtc gctgatcgtc ttcgcctgcc tgatggaagg
     2101 cctgttcttc tacgtgggct tcacgcagat cctggcgctg ggccggcaga acaagatgac
     2161 cggcgccgcc gagcagtaca tgtaaatcct gcgcgatgaa tccatgcact gcaatttcgg
     2221 catcgacctg atcaacacca tcaagctcga gaacccgcac ctctggacgc ccgagttccg
     2281 cgaggaaatc cgcgcgctgt tcaagaaggc ggtcgatctc gaatacgcct acgccgagga
     2341 caccatgccg cgcggcgtgc tgggcctcaa tgcgcccatg ttcaaatcgt atctgcgctt
     2401 catcgccaat cggcgctgcc agcagatcgg catcgaaccg ctgttccccc aggaagaaaa
     2461 ccccttcccg tggatggcgg aaatgatcga cctgaagaat gaacgcaact ttttcgaaac
     2521 ccgtgtgatc gaataccaga ccggcggcac gctgaactgg gaataagccg aacacaccgc
     2581 ccgcctgatg gcccggcggt gtttctgcct ccctctcctt agctgcacgg tgtggcatcc
     2641 ggccacgcaa gaccatacgg cagacacagc gcaaccgtct gccggcgcca tttgaaatgg
     2701 gtcgcgccgc atctgtctca tacagggcgg gatctatatc aaatggccgt gctgaattca
     2761 ttagcgcaag ccgtagccgt acttccgctc gcacagggtc gtggcatgcg ccgatgaata
     2821 acccgggcgc ggttcgtcat ccgacggatc gtgacacggg tttacgttct gggacccctg
     2881 aacctaagga gcatgaccat ggcaactgcc aaaaaagccg ccaagaaggc ggtgaagaag
     2941 accccggcca agaaagccgt caagaaagct gtcaagaaag tcgccgccaa gaaggcagtg
     3001 gtgaagaaag ccgctgtcaa gaaggttgcg gtcaagaagg tagcgaagaa ggccgtcaag
     3061 aaggtcgcca agaaggcggt caagaaagtc gccgccaaga agcccgctgc caagaaggtc
     3121 gccaaaaagg cggccaagaa ggctgtagcc aagaaggccg tcaagaaggt tgccaagaag
     3181 gctgccaaga agaccgccaa aaaggccgcg aagaaggctg cgaagcctgc tgcgaaaaag
     3241 cctgccgcca agaaggctgc gcccaagaag cccgccacgc cgccctcgac ggctgctgcc
     3301 ccgggtgcca agacggcgct caacccggcc gcttcctggc ccttcccgac cggcggccgt
     3361 ccctaatcgg accccgctgc gttgatgcag caaagccgcc tggcctggcc aggcggcttt
     3421 ttcattaccg gccaaagctg ggccagtatg cgacaatgaa aaccttcaac ttcatgcaag
     3481 gtaaggtaca cggcgcatta tgatcggcga catcgcgcgg ttcctgctcg acctctgttt
     3541 tacgcttttc ggcgcggcgc tcatcctgcg cgcctggatg catgctgtgc gcctgccccc
     3601 cttcaacccc gtggcgcgcg gcgtctacca ggccagcaac tggctgatcc tgccgctgcg
     3661 caagctcatc cccgccacca gccggatcga ctggacgagc ctggcggccc tgtggctgac
     3721 ggccctggtg tacctgctgc tggcctggct ggtgagcgtg ggcgcgctga tcccgccggc
     3781 gctcttgcct tccgttgcgg ccagcgccct gctgatggcc gtgaagtggg cgctcaacct
     3841 ggtcgtctgg atgacgctgg cccaggccgt gctgtcgtgg gtcaacccgc tctcgcccct
     3901 gatgcccatc ctgcagacgc tgacggctcc cctgctcggc cccatccggc agatcctgcc
     3961 gcgcatgagc gtggacttct cgccgctgct gctgctggtg atcgcccagg tgctgctgat
     4021 ggtgctgacc cgcgtgggct atgggctgat gggcatctag ccatcgctcg cggcaagagc
     4081 agcccgcaaa gcaaaacccc ggcacgaggc cggggtttcg ttttgcattt ttgtttgcag
     4141 ggcaggcatg gcggcaaggc catgcggcac gaacctacat cggcgccacg cgcgtggggc
     4201 cggcgccgct gatgacttgc acgcgctggc ccacgcccag gggctggtcg gcttcctgcg
     4261 ccacgacgcg ggtttcgccg ttgtccaggc gcacggtgat ttcgtagccg gaagtcttgc
     4321 ccacctggtt ttcgacggcg ttgcccgcca ccgcgcccag gatggcgccg cccacggtcg
     4381 ccagcgcatt gcccgagccc cggccgatgc cgttgcccgc cacgccgccc agcacgccgc
     4441 cggcgatcag gccgaccccg gaggagcggt cgttctggat ggtgatgggc cgcatggcga
     4501 cgatagtgcc cgtgcgcacg atctgttcgc gctgcgcctg accgtaggta tagacgctgc
     4561 tcgaagcgct ccggtcggcg cagccggcca gcacggacag ggacaagccc agcgcggcga
     4621 cggccgtcca gcggccggcg cgcacgaaag aggaagaatt cgattgcatc attttgggag
     4681 ctcctgaaac ggaacgtttt cgcggcgggc tcgaatttgc cggagaaaca gagctgattt
     4741 tagccctgtc cgccagaagc cggcgtccat catgaaaccg ggcgcaacca ctgcgaagca
     4801 gcgcgcccgg ccggcggcgg gttcaggccg gcagatggat gccgtaggag gcgtgcagca
     4861 ggccgtcgat ctcgccgcgg gcgggcttgt gattctgcgc cccgcgcgaa gcgatcttga
     4921 tggcgcccat cacgctggcc aggcggcagc tgtccagcca gttccagccc tgggtcaggc
     4981 cgtacaccag gccggcgcgg tgcgagtcgc cgcagccggt ggggtcgacc acggcggccg
     5041 ccttgacggg cggcacttcg atcgtttcgc cgccggtcat cagcgtggcg ccttcgccgc
     5101 cgcgcgtcac gatgacggcc tgcatggtct tggcgatatc ggcgatctgg cgaccggtgc
     5161 gctgctcgat cacgctggcc tcgtagtcgt tggcggtgag cacctgcgcc agcctgatca
     5221 tgcgctcgat gtcggcgccg tcgaacagcg gcatggcctg gcccaggtcg aaaatgaagg
     5281 ggatgccgag cttgttcagg cgctcggcgt gggcgaacat gccctccttg gcgtcgggcg
     5341 aaaccacgcc ccacacggcg cgggtatcgg agccgaagtc gttttgcgcc gacatggcca
     5401 tggcgcccgg gtggaaggcg gtgatctggt tgtcgtcgag gtcggtcgtg atccagcact
     5461 gcgcggtcag ggtgccgggc acgaccttga cgtgcgccgt gctgatgccc aggtcggtca
     5521 ggcgctgcag gtatccgccg gcgtcttcgc ccaccgaggc cacgggcagc ggcgcgccgc
     5581 ccagcagctt caggttgtag gccacgttgc cggcgcagcc gccgtactcg cggcgcagcg
     5641 aaggcaccag gaaggacacg ctcagcgtgt ggatgcggtc gggcaggatg tgttcgccga
     5701 aacgcccttc gaacaccgcg atggcgtcga aggccatcga gccgcaaacc agtaccggag
//
LOCUS       DASWPO010000170        14208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_485634, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000170 DASWPO010000000
VERSION     DASWPO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14208
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485634"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..826
                     /locus_tag="VGC69_09230"
     CDS             149..826
                     /locus_tag="VGC69_09230"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY0295497.1"
                     /translation="MTALPDAFFNRDACQLARELIGKVIRRKLGRLWLSARIIETEAY
                     YLGEKGSHASLGYTHKRRALFMDGGTIYMYHSQGGDSLNFSAAGPGNAVLIKSAHPWL
                     DAVSGPRSLARMQALNPDARGNPRDPRKLCAGQALLCRSLALKIADWDAQGFDPARFY
                     VEDVGAAPARLVNAPRLGIPLGRDEHLFHRYIDPDYAACCTRNPLRRGQQEGSDYVWV
                     DIQGTIL"
     gene            826..2226
                     /gene="rmuC"
                     /locus_tag="VGC69_09235"
     CDS             826..2226
                     /gene="rmuC"
                     /locus_tag="VGC69_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY0295498.1"
                     /translation="MENILLWVAAVAALLALIVSLVAALRGRDEGAQRALLDALERSG
                     RSLRDELADSQRDTRNELSESQRGLRAELSEAMRTLAAGLTQSHEDLRTALTRDARET
                     RVEHNDALGRFATQFGERLQGLVELNDRRMGEVRQTVDERLKALQADNGQRLEAMQRT
                     VDEKLHATLEQRLGESFRLVSERLEAVHKGLGEMQSLAAGVGDLKRVLTNVKSRGTWG
                     EVQLARLIEDTMTPEQYGRNVKPVPGKDVIVEFAIRLPGRRDDGEPVWLPIDAKFPKE
                     EYERLVDAQEAGDADAARAASAALARAIEVQARNITKYVEPPHTTDFAIMFLPTEGLY
                     AEVLRVPGLVDKLQGMRVNVAGPSNLAAMLNSLQMGFRTLAIEQRSSEVWKILGAVKT
                     EFGKFGESLASVKKTLDQASNKIGQTEVRTRAMLRNLREVEALPESEAVQLLGGESGE
                     GGEGSEADDADRPDEG"
     gene            complement(2260..3654)
                     /locus_tag="VGC69_09240"
     CDS             complement(2260..3654)
                     /locus_tag="VGC69_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0295499.1"
                     /translation="MDELPPPDAEFDVLVVGAGGCGLTAALAAQAAAPGASVAVVEKA
                     DRMRGNTTLSSGSIPAAGTRMQAQAGMADSPEDFIEDLQRIAGPHEMPGLTRRLAQVS
                     AELVEWLVDEAGANLTLVETYKHIGHRQYRLHSPPSRRGADLMADLARSVEARGIPLA
                     FGNPAVELLATPQGGVGGAITLTPDGERTVIGARAVILATNGFGANRALLARFCPELA
                     EAPYGGSPGSEGEAMAWGEALGADLANMGAYQAHASLADPHGSLVTWTVVEKGGIVVD
                     GQGRRFGDESLGYSAFAALELERKGPFYVVADTRVRDMTARGQEEYGELVQHKGVVEA
                     PDADALAQRLGLPADALRATLAQAAQAAAGAADAFGRSHWGLGALVAPYTATRIGPAL
                     FHTQGGLRIDDDGRVLRPGGKPIAGLYAGGGAAAGISGNRGSTGYMSGNGLLAALGLG
                     YLAGRAAVRDLEAM"
     gene            complement(3673..4665)
                     /locus_tag="VGC69_09245"
     CDS             complement(3673..4665)
                     /locus_tag="VGC69_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295500.1"
                     /translation="MAGRSRRRALLAGAAVIGCALMPIWAHADGADGFPKHAVTLIVP
                     YTPGGPTDLTGRIIGEGLSKMWGQSVIIENRPGAGGNIGSAMVAKAPPDGYTIVLGVT
                     GSHAINKWLYKNLPYDPQKDFEPIGMAAIYANAILINPSVPANSLKELVELIRKNPGK
                     YSFGSDGNGAASHLSMELVKMQAKLDLPHIPYKGSAPLLNDLMGGTVPIGITGVPSAE
                     PLVKSGKLKLLAVTTAKDYSGNNYPTIADQGFPGFDTAAFSGLFAPKGTPKAIVDKMS
                     ADLASVLAQPEVKAKMNTLGLQVQITTPKEFADFLAKQIDQWHEAVTVSGAQVD"
     gene            4989..5669
                     /gene="rpiA"
                     /locus_tag="VGC69_09250"
     CDS             4989..5669
                     /gene="rpiA"
                     /locus_tag="VGC69_09250"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY0295501.1"
                     /translation="MLSQQELKQQAADAALEFVDAVAAPGVVIGVGTGSTADLFIDGL
                     ARFKGRIGATAASSERSAARLAGHGLKVLDLNEVESMPIYVDGADEIDANLHMIKGGG
                     GALTREKIVASVAERFICIADESKLVERLGKFPLPVEVIPMAREAVARRLAALGGQPR
                     LREGFITDNGNVILDVSGLRIADAPGLEADINSYAGVVTCGLFALAGADVALLATQNG
                     IRRLERPR"
     gene            5758..6480
                     /gene="phoU"
                     /locus_tag="VGC69_09255"
     CDS             5758..6480
                     /gene="phoU"
                     /locus_tag="VGC69_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY0295502.1"
                     /translation="MPDHTNKQFDSDLEFVRSQFLIMGGVVEAMVNEAIEALSTSDLV
                     LVDKVREREKEVNRSEVEIDERISLLLARHQPTAIDLRTLLAVSKMLTDMERSGDEAE
                     KIAVAVRRMQESGQPHLPLIELRHMSDKVASMLRQMLDAFARLDPVQAAAVVRSDKEV
                     DREWKAALRNLITYMMEDPRTISHAIDLIFISRSLERIGDHAKNMAERVIYMVRGADV
                     RHTGVKNTERQARGEGEATTEA"
     gene            6548..7318
                     /locus_tag="VGC69_09260"
     CDS             6548..7318
                     /locus_tag="VGC69_09260"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0295503.1"
                     /translation="MRQRRNGAPRAIGRPLRDKYDEDELTALYYGIGLHLGKPVTQNA
                     RGDLLGNVMNVGDPRKKSTRVYETNAYLPYHSDVSDVVGLLCLRKAKAGGLSCLVSSA
                     TIYNEMLGKHPEFLGYFYCPIYYDHMGEELPSLTPIFSYHEGKLSSRYLRAYIELGHE
                     RRGIPLSRVAIEALDLFDALMNDADLRLDMMLEPGDIQFCNNYAIMHSRTHFEDFEEP
                     ARRRKLSRLWLKMPNARTLAPDFPGRNGIPATGAMPDF"
     gene            complement(7350..8132)
                     /locus_tag="VGC69_09265"
     CDS             complement(7350..8132)
                     /locus_tag="VGC69_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0295504.1"
                     /translation="MTERDKLPPLERYVRILELLAAFPDGLTLADVARMLDLPKTSAH
                     RLLGTMQDSDLIDMAAGASSTYVLGQRIARLAYRGAGTQWLDTLVRPYLRDLSAETGE
                     TCYLARLDGLLVTSVVMEAPDTPWRGFVLPGKDMPAHAAASAKAILAFQDETSIMEIL
                     SQPLPRLTSKTRTSTEDILAEYEQVRRQGYATCIGEIDEGLAALGVPVHIPNVGVIYS
                     VGVTGPLQRIMDQQPHLTAQTMARCAERIAHALSVGLARRPG"
     gene            8336..9484
                     /locus_tag="VGC69_09270"
     CDS             8336..9484
                     /locus_tag="VGC69_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295505.1"
                     /translation="MKISLFRFGAAVRVVTLAASLAASGVAWAQDTIEVGATVPLTGP
                     LSMTGKQYYNTLKLAEEDINKAGGIQGKKLSFVFEDAQASNGAAVNAFVKIVQEHKPP
                     FVFLSSYSTQNIAVTPEVAKAGIPVMYAGGADAVSDAKSPWMFRIRPQDSTAAVAMAR
                     FVKENLKATKPGILYLQNDFGQGGATAAQRFLEEQGIKPVGMEAYGQNDKDMSAQLLS
                     LKNKGADVLLAFVYPQDGALLLRQIKMLGLKQPVVASSAAFVPAAMQLLSPADLDNVW
                     GVIDADLESTPAGKDFTARYKAKYQIDADPYGAAYYDGAMLMARAMNAVGTDPAKLRD
                     YLAGVKGYQGVSHVYSIDAQGNGVHDVAVVKFKPGTKDMQFVDSISVQ"
     gene            9543..10418
                     /locus_tag="VGC69_09275"
     CDS             9543..10418
                     /locus_tag="VGC69_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0295506.1"
                     /translation="MQDFIQLIVNGVALGSVYALAALGFVIVYSATGIVNFSAGQFVM
                     LWTFFGVTTVLQAQLPTIEAYPLAIAMMAAFGVLFYLAVHFPLQGRPVVAVIIGTVAV
                     GIAIQNAALLIWGAWPFRIPSPFGDGTVSLGGSVITVHALATIVITMLLIAGLHVLLH
                     RTSLGRSMRAVAQDVETARLMGLPVALLLGFSWVLAAMFAGFAGLLVAPMWFADVNMG
                     DPIALKAFAATIIGGFGSVPGAIVGGLSVGLIEILGASYLSSTYKDLLAFGVMILFLL
                     VRPQGIFGERVADRG"
     gene            10420..11373
                     /locus_tag="VGC69_09280"
     CDS             10420..11373
                     /locus_tag="VGC69_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0295507.1"
                     /translation="MNQRKLLLSSVLLLVLAALLPLVLADYGTRLLNAALVAAVAVLG
                     LNFVFGYAGLISLGHAAFVGVGAYTVALLTTAIGVNSWVAVPCAVVLSALSAFVTGWI
                     LLRIKGHYLALATLGLNVSFTIVASNWMALTGGSNGVAGIPTLSLAGIELGDERRFYW
                     FALAVLALLTWLAARIRGSYAGRAMVAVRDDEIATAMSGLAIARVKVAAFTLGGAYAG
                     VAGCLFAFHMKFVSPDDFNFSHSITYLSMLIVGGEGTLLGSILGAVGVTLLPEVLRGL
                     GNAYLLVFGLLVLLVLAVLPNGLAGLLARWRAPVGAQGAKP"
     gene            11370..12149
                     /locus_tag="VGC69_09285"
     CDS             11370..12149
                     /locus_tag="VGC69_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295508.1"
                     /translation="MSLLQVSSVSKRYGGVQALSDINLEIQPDQFFAVIGPNGAGKST
                     LLNVLTGLVPPSSGSIRFEGRDLTASGTHAIVRAGIGRIFQSGRLFSRLTVLENVMMG
                     SAMAEPPSVWATVLRPGATRRREAVAREAAMGMLESFGLQALADRSIGSLSYGNRRLV
                     EMARVMAARPRLLLLDEPAAGLNSGEVENLMEMLRRLRGAHRLSVVLIEHNMGMVMRL
                     AERIAVLNFGSKLAEGTPQEIQGNPDVLEAYLGKGYQNVEM"
     gene            12136..12837
                     /locus_tag="VGC69_09290"
     CDS             12136..12837
                     /locus_tag="VGC69_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295509.1"
                     /translation="MLKCSGLGAAYGDIVALRGVDMHVAQGEAVALIGVNGAGKTTLL
                     RAISGLLPSTGDIAFDGASLGRLSPERRVRTGIAHVPEGRRIFPGLTVRENLEVGASV
                     RRGKGDVAADFDRIFDLFPRLAERHRQLGWSLSGGEQQMLAIGRALMASPRLLLLDEP
                     SLGLAPRLAEEVYTRIATINEGGLTVLLVEQNTVLALDVADRGYVLENGTIVLADAAA
                     SLRSNPRVRDAYLGR"
     gene            12891..>14208
                     /locus_tag="VGC69_09295"
     CDS             12891..>14208
                     /locus_tag="VGC69_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0295510.1"
                     /translation="MYPDTASKSYALHQRAKRSLPGGNTRTTVFMSPYPVYAARGEGC
                     RVYDVDGNALIDCINNFTSLIHGHAHPAIVETAQRHVTQGSAFGLPTESEIALAEVLC
                     EQVPSIEHVRFTNSGTEAVMMALKAARAYTGRPKIAKVEGAYHGSYDYAETSLDAEPA
                     NWGGDAPNSVPYAKGTPQGVLSDVVVLPFNDVARTEALLRRHGSELAGVLIDLVPNRV
                     GLVPASPDYLAMLRRVTRELGILLIVDEVITLRLARGGAQTRLGVEADLTTVGKIMGG
                     GFPVGGVGGRKDIMAVFDPTSGRPAVPHGGTFSANPMSMACGLRAMELLTGEAYAQLD
                     HMGDTLRQALNQAFHNAGVAGQATGLGSLLKLHLRDQPITDYRSAYPAKAERDAMAAI
                     NVKLLNRGVLMAGYGLIALSTAMRDADIAHIAEAAHDSLREWVAERS"
BASE COUNT         2372 a         4595 c         4691 g         2550 t
ORIGIN      
        1 ccagtggggg cgagcatcgg aattccgcag ggcagcctcg gcggctgaag aatccgatac
       61 cggccgaaga acccgaatac cgcctgccat gagcgcgcaa ccctggatgt aacattggcc
      121 ccctcccggt tttcagaaaa agtccttcat gaccgcgctg cctgacgcct tcttcaaccg
      181 cgacgcctgc cagttggcgc gcgagctgat cggcaaggtc atccgccgca agctgggacg
      241 cttgtggctg tcggcccgca tcatcgagac cgaggcctac tatctgggcg agaagggcag
      301 ccacgcttcg ctgggctata cccacaagcg ccgcgccctg ttcatggacg ggggcacgat
      361 ctacatgtac cactcccagg gcggcgattc gctgaacttc agcgccgcgg ggcccggcaa
      421 tgccgtgctg atcaaatccg cccacccatg gctcgacgcc gtgtccggcc ccaggtcgct
      481 ggcgcgcatg caggcgctca accccgacgc gcggggcaat ccccgcgacc cgcgcaagct
      541 gtgcgccggg caggcgctgc tgtgccgctc gctcgccctg aaaatcgccg actgggacgc
      601 gcaaggcttc gaccccgccc ggttctatgt cgaggatgtg ggcgccgcgc cggcccgcct
      661 cgtcaacgcc ccccggctgg gcatcccgct gggacgcgac gaacacctgt tccaccgcta
      721 catcgatccc gactacgccg cctgctgcac ccgcaatccg ctgcgccgcg ggcagcagga
      781 gggcagcgac tacgtctggg tcgacattca aggtacgatt ttgtgatgga aaacattctg
      841 ctttgggttg ccgccgtggc cgcgttgctg gccttgatcg tgagcctggt cgccgccttg
      901 cgcggccgcg acgagggcgc ccagcgtgcc ttgctcgatg cgctggaacg ttcggggcgc
      961 agcctgcgcg acgaactggc cgacagccag cgcgataccc gcaacgaact ctccgagagc
     1021 cagcgcggcc tgcgcgccga gctgtccgag gccatgcgca ccctggcggc cggcctcacg
     1081 cagagccatg aagacctgcg caccgcgctg acccgcgatg cccgcgagac ccgcgtcgag
     1141 cacaacgacg ccctgggccg cttcgccacc cagttcggcg agcgcctgca gggcctggtc
     1201 gagctcaacg accgccgcat gggcgaggtg cggcaaaccg tggacgagcg cctcaaggcc
     1261 ctgcaggccg acaacggcca gcgcctggag gccatgcagc gcaccgtcga cgaaaagctg
     1321 cacgccacgc tcgagcagcg cctgggcgaa tccttccgcc tggtctccga gcgcctggaa
     1381 gccgtgcaca agggcctggg cgagatgcag agcctggcgg ccggggtggg ggacttgaag
     1441 cgcgtgctga ccaacgtcaa gtcgcgcggc acctggggcg aggtgcagct ggcccgcctg
     1501 atcgaggaca ccatgacgcc cgagcagtac gggcgcaacg tcaagccggt gccgggcaag
     1561 gacgtcatcg ttgaattcgc catccgcttg ccgggacgcc gcgatgatgg cgaaccggtc
     1621 tggctgccca tcgacgccaa gttccccaag gaagaatacg aacggctggt cgatgcccag
     1681 gaggccggcg atgcggacgc ggcccgcgcg gcgtcggccg cgctggcgcg cgccatcgag
     1741 gtccaggcgc gcaacatcac caagtacgtc gagccgcccc acacgacgga cttcgccatc
     1801 atgttcctgc ccaccgaagg cctgtatgcc gaggtcctgc gcgtgccggg cctggtcgac
     1861 aagttgcaag gcatgcgcgt gaacgtggcc ggcccgtcga atctggccgc catgctcaac
     1921 agcttgcaga tggggtttcg caccctggcc atcgagcagc gttcgtccga ggtctggaag
     1981 atcctggggg cggtcaagac cgaattcggc aaattcggcg agtcgctggc cagcgtcaaa
     2041 aagaccctgg accaggccag caacaagatc ggccagactg aggtgcgcac ccgggccatg
     2101 ctgcgcaatc tgcgcgaggt agaggccctg ccggaaagcg aggccgtgca actgctgggt
     2161 ggtgaaagtg gcgaaggcgg tgagggcagc gaagccgacg acgcggatcg gccagacgag
     2221 gggtgagcgg gtggcgcggc cggggccgcg cctgccgggc tacatggcct cgaggtcgcg
     2281 cacggcggcg cgtcccgcga gatagcccag ccccagcgcg gccagcaggc cgttgccgga
     2341 catatagccg gtgctgcctc gattgccgct gatgccggcc gccgcgccgc cgcccgcgta
     2401 caggccggct atgggcttgc cgccggggcg cagcacgcgg ccgtcgtcat cgatgcgcag
     2461 gccgccttgc gtgtggaaca gcgccgggcc gatgcgggtc gcggtgtagg gcgcgacgag
     2521 cgcgcccagg ccccaatggc tgcggccgaa agcatccgca gcgccggcgg ccgcttgcgc
     2581 ggcttgcgcc agggtcgcgc gcagcgcatc ggccggcagg cccaggcgct gcgccaaggc
     2641 gtccgcgtcg ggcgcctcga cgacgccctt gtgttggacc agttcgccgt attcttcctg
     2701 gccccgggcc gtcatgtcgc gcacgcgggt gtcggcgacg acatagaagg ggcccttgcg
     2761 ctccagctcg agcgcggcga acgcggaata gcccaggctt tcgtcgccga agcggcggcc
     2821 ctgcccatcg acgacgatcc cgcccttttc gaccacggtc caggtaacga gcgaaccgtg
     2881 cgggtcggcc aggctggcat gggcctggta ggcgcccata ttggccaggt ccgcgcccag
     2941 tgcctcgccc caggccatgg cctcgccctc gctgcccggc gagccgccat agggcgcctc
     3001 ggccagttcg gggcaaaagc gcgccagcaa cgcccggttg gcgccgaagc cgttggtggc
     3061 caggatgacg gcgcgcgcgc cgatgacggt gcgttcgccg tccggggtca gggtgatcgc
     3121 gccgcctacc ccgccttgcg gcgtggccag cagttcgacg gctgggttgc cgaaggccag
     3181 cgggatgccg cgcgcctcca ccgaacgcgc caggtcggcc atcagatcgg cgccacggcg
     3241 cgagggcggc gaatgcaggc ggtattggcg gtggccgatg tgcttgtagg tctcgaccag
     3301 cgtgaggttt gcgcccgcct cgtcgaccag ccattcgacc agctcggccg agacttgcgc
     3361 cagccggcgg gtcaggccgg gcatttcgtg cggaccggcg atgcgctgca ggtcctcgat
     3421 gaagtcctcc ggactgtcgg ccatgcccgc ctgggcctgc atgcgcgtgc cggcggcggg
     3481 gatcgaaccg ctggacagcg tggtgttgcc gcgcatccgg tccgctttct cgacgaccgc
     3541 taccgaggcg ccgggcgcgg cggcctgggc cgcgagcgcc gcggtcaggc cgcagcctcc
     3601 cgcgccgacg acgagcacgt cgaattccgc gtccggcggc ggcaactcgt ccacattcct
     3661 gatccgcatt gttcagtcga cctgggcgcc ggacaccgtc accgcttcat gccattggtc
     3721 gatctgcttg gctagaaagt cggcgaattc cttcggcgtg gtgatctgca cctgcaggcc
     3781 gagcgtgttc atctttgcct tcacctcggg ttgagccagc acggaggcca ggtcggccga
     3841 catcttgtcg acgatggcct tgggcgtgcc cttgggcgcg aacaggcccg agaacgcggc
     3901 ggtatcgaag cccgggaagc cctggtcggc gatcgtggga tagttgttgc cgctgtagtc
     3961 cttcgcggtg gtgacggcca gcagcttgag cttgcccgac ttgaccagcg gctcggcgga
     4021 tggcacgccc gtgatgccga tgggcaccgt gccgcccatc aggtcgttga gcagcggcgc
     4081 gctgcccttg tacgggatgt gcggcaggtc gagcttggcc tgcatcttca ccagttccat
     4141 cgacaggtgg ctggccgcgc cgttgccgtc ggagccgaag ctgtacttgc cggggttctt
     4201 gcggatcagc tcgaccagtt ccttgagcga attggccggc acgctgggat tgatgagaat
     4261 ggcattggcg tagatggcgg ccatgccgat cggttcgaaa tccttctgcg gatcgtaggg
     4321 gaggttcttg tacagccatt tgttgatggc atgcgagccc gtgacgccca gcacgatggt
     4381 gtagccgtcg ggcggcgcct tggcgaccat ggcggaaccg atgttgccgc ccgcgccggg
     4441 acggttttcg atgatcacgc tctggcccca catcttggag agcccttcgc cgatgatgcg
     4501 gccggtcagg tcggtcgggc cgccgggcgt gtagggcacg atcagggtga cggcgtgctt
     4561 gggaaagccg tcggccccat cggcatgcgc ccagatgggc atcagggcgc agccgatgac
     4621 cgccgcgccg gcgagcaggg cgcggcggcg cgaacgtcca gccatggatt gagcgtgctt
     4681 gctgcgaaac tgcatcatca tttcccctat atgtgtgaac aaattttgaa gtgtcgacaa
     4741 tataggattc gtcttggcaa gggtcaacct tcagtggcgc aatttccgcg taaaaagagt
     4801 gttaattagg gtaaaccagg gtaattttcc tgggttgaat ataaaactgt cgacaaattg
     4861 actggacaga taggacggga aacggcctgg aatgcctctc ccggcgccag gctgggcaca
     4921 ggtccgcgtt cacgtatctg ctacattgcg ggtggctttc cacaatgcgt tcaacggccc
     4981 gacatcccat gctctcccaa caagaactca agcaacaggc cgccgacgcg gccctggaat
     5041 tcgtcgatgc cgtggcggct cccggtgtgg tcatcggcgt gggaaccggc tccaccgccg
     5101 acctcttcat cgacggcctg gcgcgtttca agggccgtat cggcgccacc gccgccagct
     5161 ccgagcgcag cgcggcgcgg cttgccggcc atggcttgaa agtcctcgac ctcaacgaag
     5221 tcgagtccat gcccatctat gtcgacggcg ccgacgagat cgacgccaac ctgcacatga
     5281 tcaagggcgg cggcggcgcg ctcacgcgcg agaagatcgt cgcctccgtg gccgagcgct
     5341 tcatctgcat cgccgacgaa tccaagctgg tcgagcgcct gggcaagttc ccgctgccgg
     5401 tcgaagtcat ccccatggcc cgcgaggccg tggcgcgtcg cctggcggcg ctgggcggcc
     5461 agccgcgcct gcgcgagggc ttcatcaccg acaacggcaa cgtcatcctg gatgtgagcg
     5521 gcttgcgcat tgccgatgcc ccggggctcg aggccgacat caacagctac gccggcgtgg
     5581 tcacctgcgg cctgttcgcc ctggccggcg ccgacgtcgc cttgctggcg acgcaaaacg
     5641 gtattcgccg cctggaacgc ccccgctagt ccgattcata atttcgtcat acttacgtca
     5701 taagctgctt agctgccacc aaaggcccta ggctcgtcca tcatcgaagg aacacgcatg
     5761 ccggatcaca ccaacaaaca gttcgacagc gacctggagt tcgtccgttc gcagttcctc
     5821 atcatgggtg gcgtcgtcga agccatggtc aacgaggcga tcgaggcctt gtccaccagc
     5881 gacctggtcc tggtcgacaa agtccgcgag cgcgaaaagg aagtcaatcg cagcgaagtc
     5941 gagatcgacg agcgtatcag cctgctgctt gcccgccacc agcccacggc catcgacttg
     6001 cgcacgctgc tggccgtgtc caagatgctc accgacatgg agcgttcggg cgacgaggcc
     6061 gagaagatcg ccgtcgccgt gcgccgcatg caggagagcg gccagcccca cctgccgctg
     6121 atcgaattgc gccacatgag cgacaaggtc gccagcatgc tgcgccagat gctcgacgcc
     6181 ttcgcccgcc tcgacccggt ccaggccgcc gccgtcgtgc gcagcgacaa ggaagtcgac
     6241 cgcgaatgga aggcggccct gcgcaatctc atcacctaca tgatggaaga tccgcgcacc
     6301 atctcgcatg ccatcgacct gatcttcatc tcgcgctcgc tcgagcgcat cggcgaccat
     6361 gccaagaaca tggccgagcg cgtcatctac atggtgcgcg gcgcggatgt gcgccacacc
     6421 ggcgtcaaga acaccgagcg ccaggcccgc ggcgagggcg aagccaccac cgaagcctga
     6481 ttttcttcca ggctcaaaag cagcctcgta ccgggtggac cccgttacga ggctgttttg
     6541 ctttaggttg cgtcagagac gcaatggcgc gcccagagcg atcggacggc ccctgcggga
     6601 caagtacgac gaagacgagc tcaccgccct ctattacggc atcggcctgc acctgggcaa
     6661 gcctgtcacg caaaacgccc gcggcgacct gctgggcaat gtgatgaacg tgggcgaccc
     6721 gcgcaagaaa agcacgcggg tgtacgaaac caatgcctat ctgccttatc actccgacgt
     6781 gtcggacgtc gtgggcctct tgtgcctgcg caaggccaag gctggcggcc tgagctgcct
     6841 ggtgagctca gccacgatct acaacgagat gctcggcaag caccccgaat ttcttggcta
     6901 tttctactgc ccgatctact acgaccacat gggcgaggag ctgcccagcc tcacgccgat
     6961 cttcagctac cacgagggca agctcagcag ccgctacctg cgcgcctaca tcgagctggg
     7021 ccacgagcgg cgcggcattc ccttgtcgcg cgtggccatc gaagcgctcg acctgttcga
     7081 tgcgctgatg aacgacgccg atctgcgcct ggacatgatg ctcgaaccgg gcgacatcca
     7141 gttctgcaac aactacgcca tcatgcattc gcgcacccat ttcgaggatt tcgaagagcc
     7201 cgcccggcgc cgcaagctgt cgcgcctgtg gctcaagatg cccaacgcgc ggacgctggc
     7261 gcccgacttc ccgggccgca acggcattcc ggccacgggc gcgatgccag atttttgatt
     7321 cttgaacata ccccacggac tgtgcggcgt tacccaggcc ggcgcgcaag tcctacggac
     7381 agagcatgtg caatgcgttc ggcgcagcgt gccatggtct gtgccgtgag atgcggttgc
     7441 tggtccatga tgcgttgcag cggacccgtg acgccgacgc tgtatatcac ccccacattg
     7501 ggaatgtgca ccgggacgcc cagcgcagcc agaccctcat cgatttcacc gatacaggtt
     7561 gcatagcctt gtcggcggac ttgctcatac tcggccagga tgtcctcagt cgaagtcctg
     7621 gtttttgagg taagcctggg caagggctgg ctcaagattt ccatgatgct cgtttcatcc
     7681 tggaaggcca gaatggcctt tgcagaggct gcggcatgtg cgggcatgtc tttccccggc
     7741 aatacgaagc cgcgccatgg cgtatccggt gcttccatca cgacagatgt cacgagcagc
     7801 ccatccaatc gcgccaggta acaggtctcg cctgtttccg ctgacaagtc gcgtagatag
     7861 ggcctgacca gcgtatcgag ccattgcgtg cccgcgccgc gataggccag cctggcgatg
     7921 cgctggccca gcacataggt gctggacgca ccggcggcca tgtcgatcag atccgaatcc
     7981 tgcatggtgc ccagcaagcg gtgcgcgctg gtcttgggca gatccagcat gcgcgccacg
     8041 tccgccagcg tcaatccgtc gggaaaggcc gcaagcagtt ccaggatgcg tacgtatcgt
     8101 tccagggggg ggagtttgtc tcgttccgtc atgtgaagtt tcgtggactg caatgcgccg
     8161 gtgatgcggt tcttttgcaa agagccaggg gaaaacccga gcttgccgat gaaggcgctc
     8221 ttttcttatc ataccgaaaa tcagtattaa gttccgaaaa tcggaacgat caacggggct
     8281 gtggcactga agccgcgtgc cttcggttca atccagctct ctaggagtgc catacatgaa
     8341 gatcagcctt ttccggtttg gtgccgcggt tcgcgtggtg acgctggcgg caagtctcgc
     8401 cgcctctggc gtggcgtggg cgcaggatac catcgaggtc ggcgccacgg tgccgctcac
     8461 cgggccgctg tcgatgacgg gcaagcagta ctacaacacg ctcaagcttg ccgaagagga
     8521 catcaataag gctggcggca tccagggcaa gaagctgagt ttcgtcttcg aggacgcgca
     8581 ggcatcgaac ggcgcggccg tcaatgcctt cgtcaagatt gtccaggagc acaagccgcc
     8641 tttcgtcttc ctgagcagct acagcacgca aaacatcgcg gtgacgcccg aagtcgccaa
     8701 ggcgggcatt cccgtcatgt acgctggcgg cgccgacgcc gtgtcggacg ccaagagccc
     8761 ctggatgttt cgcatccgcc cgcaggacag caccgcggcc gtcgccatgg cgcgtttcgt
     8821 caaagagaat ctcaaggcca cgaagccagg catcctctac ctgcagaacg atttcgggca
     8881 gggcggcgcg acagcggccc agcggttcct ggaagaacag ggcatcaagc cggtgggcat
     8941 ggaagcctac ggccagaacg acaaggacat gtcggcccag ttgctgagcc tgaagaacaa
     9001 gggcgccgat gtgctgctgg ccttcgtgta cccgcaagat ggcgcgctgc tgctgcgcca
     9061 gatcaagatg ctgggcttga aacagccggt ggtcgcgtct tccgcggctt tcgtgccggc
     9121 ggccatgcaa ctgctgtcgc ccgccgacct agacaatgtc tggggtgtga ttgatgccga
     9181 tctggaaagt acgcccgcgg gcaaggactt cacggcccgc tacaaggcca agtaccaaat
     9241 cgacgccgac ccctacggcg cggcctacta cgacggcgcg atgctgatgg cgcgcgccat
     9301 gaacgcggtc ggtaccgacc cggccaagct gcgcgactac ctcgccggcg tcaagggcta
     9361 ccagggcgtt tcgcacgtct acagcatcga cgcccagggt aatggcgtgc atgacgtggc
     9421 ggtggtcaag ttcaagccgg gcaccaagga catgcagttc gtcgattcga tctcggtgca
     9481 ataaaaccgc gcccgcggca agggccgggc ggtgcagccg cccggcgatt cagggggaag
     9541 ctttgcagga tttcatccaa ctcattgtca acggtgtcgc gctgggcagc gtttatgcgc
     9601 tggcggcgct cggcttcgtg atcgtctatt ccgcgacggg catcgtcaat ttctccgccg
     9661 gccagttcgt gatgctgtgg acgttcttcg gcgtcaccac cgtgttgcag gcgcaactgc
     9721 ccacgatcga ggcttatcca ctggcgatcg ccatgatggc ggcctttggc gtgctcttct
     9781 acctggcagt ccatttcccg ctgcagggcc ggccggtagt ggccgtcatc atcggcacgg
     9841 ttgccgtggg catcgccatc cagaacgcgg cgctgctgat ctggggggcc tggccgttcc
     9901 gcatcccttc gccttttggc gatggcacgg tctctctggg cggcagcgtc atcacggtcc
     9961 atgccctggc caccattgtc atcacgatgc tgctcatcgc cgggcttcac gtgctgttgc
    10021 accggaccag cctgggacgt tccatgcgcg ccgtggcgca ggatgtcgag accgcacgcc
    10081 tcatgggatt gcccgtggcg ctgctgctgg gattctcctg ggtactggcc gcgatgttcg
    10141 ccgggttcgc cggactgttg gtggcgccca tgtggttcgc cgacgtcaat atgggcgatc
    10201 ccattgcgct caaggccttc gcggccacca tcatcggcgg cttcggcagt gtgccgggtg
    10261 ccatcgtggg cggcctgtcg gtggggctga ttgaaatcct gggagcgtcc tacctgtcgt
    10321 ccacgtacaa ggatctcctg gctttcggcg tgatgatcct gttcctgctg gtgcgtccgc
    10381 agggcatttt cggcgaacgt gtcgcagaca ggggctgata tgaatcaacg caagctattg
    10441 ctgagcagcg tgctgctgct ggtgctggcc gctcttttgc cgctggtgct ggccgattac
    10501 ggcacgcggc tgctcaacgc ggcgctcgtc gccgccgtcg ccgttcttgg actgaatttt
    10561 gtctttggct atgccggcct gatttcgctg gggcacgccg ccttcgtcgg cgtgggggcc
    10621 tatacggtgg cgctgctgac aactgcgatt ggcgtcaatt cctgggtggc ggtgccgtgc
    10681 gcggtggtgc tgtcggccct gtcggctttc gtcacgggat ggatactgct gcggatcaag
    10741 gggcattacc tggcactggc gacgttgggt ctgaatgtca gtttcaccat tgtcgcctcg
    10801 aactggatgg cgcttacggg cggtagcaat ggcgtggccg gcattcccac cttgtcgctg
    10861 gcgggcatcg aactgggcga cgaacggcgt ttttactggt tcgcattggc agtgctggcg
    10921 ctgttgacct ggctggccgc gcgcatccgg ggctcatatg ccggccgtgc catggttgcg
    10981 gtgcgtgatg acgagatcgc cactgcgatg tcgggcctgg ctatcgcgcg cgtcaaggtg
    11041 gcggccttca cgctgggcgg cgcctatgcc ggcgtggccg gttgcctgtt cgcgttccat
    11101 atgaagttcg tatctccgga cgacttcaac ttttctcatt ccatcactta tctgtccatg
    11161 ctgatcgtgg gcggagaggg cacgctcctg ggcagcatat tgggtgcggt cggcgtgaca
    11221 ctgctgcccg aggtgctgcg cggcctgggc aatgcctacc tgctggtctt cggcctgctg
    11281 gtactgctgg tgctggccgt gctgcccaat ggcctggctg gactgttggc acgctggcgc
    11341 gcaccggttg gcgcacaggg agccaaacca tgagtctttt gcaagtcagc agcgtgagca
    11401 agcgctacgg cggcgtgcag gcgctgtccg atatcaacct ggaaatccag cccgaccagt
    11461 ttttcgcagt catcggtccc aacggcgcag gcaagagcac gctgctcaat gtgctcaccg
    11521 ggctggtgcc gccgtcctcg ggcagtatcc ggttcgaagg gcgcgacctg acggcctcgg
    11581 gcacccatgc cattgtccgt gccgggatcg gccgcatttt ccagagcggc cgcctgttca
    11641 gccggctgac cgtgctggaa aacgtcatga tgggttcggc catggccgaa cccccatccg
    11701 tctgggccac ggtgttgcgc cctggcgcga cccgtcggcg tgaggccgtg gcccgtgaag
    11761 cggccatggg catgctggag tcgttcggct tgcaagccct ggccgaccgt agcatcggtt
    11821 ctctttccta tggcaatcgg cgcctggtgg aaatggcccg ggtcatggcc gcgcggcctc
    11881 gtctgctgct gctcgacgag ccggcggcag gactgaattc cggcgaggtg gaaaacctga
    11941 tggaaatgtt gcgccggctg cgcggcgcgc accggctgtc ggtcgtcctc atcgagcaca
    12001 acatgggcat ggtcatgcgg ctggccgagc gtattgccgt gctcaatttc ggcagcaagc
    12061 tggccgaggg tacgccgcag gagatccagg gcaatccgga tgtgctggag gcgtatctgg
    12121 gcaaggggta tcagaatgtt gaaatgtagc ggactgggcg cagcctacgg cgacatcgtc
    12181 gccttgcgtg gcgtggatat gcatgtggcg cagggcgagg ccgtcgcgct gatcggcgtc
    12241 aacggcgcgg gcaagacgac cctgctgcgt gcgatttccg gcctcttgcc cagtactggc
    12301 gacatcgctt tcgacggcgc gagcctgggc cggctgtcgc ccgagcggcg ggtacgcacg
    12361 ggcattgccc atgtgccgga agggcgccgc atctttcctg gcctgaccgt gcgggagaac
    12421 ctcgaggtgg gtgcctcggt gcgtcggggg aagggggatg tcgctgcgga ttttgaccgc
    12481 atttttgact tgttcccgcg gctggccgaa cgccatcggc aactgggctg gtcgctgtcc
    12541 ggcggggagc agcagatgct cgccatcggt cgggcgctaa tggccagccc gcgtctgcta
    12601 ctgctcgacg agccctcgct gggcctggcg ccgcgcctgg ccgaggaggt gtacacgcgc
    12661 atcgccacca tcaacgaagg cggtctgacc gtgctgctgg tcgagcagaa caccgtgctg
    12721 gcgctcgatg tggccgatcg cggctacgtg ctcgagaacg gcaccatcgt gctggccgat
    12781 gcggccgcca gcctgcgcag caatccgcgt gtgcgcgatg cttacctggg ccgctgaagc
    12841 aatgccagcg cctggttttc cacaaaactt atccaaccga gagtagtgcc atgtatcccg
    12901 ataccgcatc gaagtcctat gcgctgcatc agcgcgccaa acggtcgctg cccggcggca
    12961 acacccgtac aaccgtcttc atgtcgcctt atccggtgta tgccgcgcgc ggcgagggct
    13021 gccgcgtgta tgacgtcgac ggcaatgcgc tgatcgattg catcaataat ttcacctccc
    13081 tgatccacgg ccatgcgcat cccgctatcg tggaaaccgc gcagcgccat gtgacgcaag
    13141 gctcggcctt cggcctgccc accgagagcg aaatcgcatt ggccgaagtg ttgtgcgagc
    13201 aggtgccttc catcgaacac gtccgcttca ccaattcggg caccgaggct gtgatgatgg
    13261 cgctcaaggc agcacgtgcc tataccggcc gtcccaagat cgccaaggtc gaaggtgcct
    13321 atcatggttc ctacgactac gccgagacca gcctggatgc ggaaccggcc aattggggcg
    13381 gcgatgcgcc caactcggtg ccctatgcca agggcacgcc gcaaggcgtg ttgtcggacg
    13441 tggtggtgtt gcctttcaac gacgtcgcgc gcaccgaggc gctgttgcgc cggcatggca
    13501 gcgaactggc cggcgtgctg atcgacctgg tgcccaatcg tgtgggcctg gtgcccgctt
    13561 cgccggacta cctggccatg ttgcgccggg ttacccggga actgggcatc ttgctgatcg
    13621 tcgacgaagt catcaccttg cgcctggcgc gtggcggtgc gcagacgcgc ctgggggtcg
    13681 aggccgacct gaccaccgtg ggcaagatca tggggggagg cttcccggtg ggcggcgtgg
    13741 gcgggcgcaa ggacatcatg gccgtgttcg accctacttc ggggcgcccg gcagtgccgc
    13801 acggtggcac tttctcggct aatcccatgt ccatggcgtg cggtttgcgg gccatggaac
    13861 tgcttaccgg cgaggcttat gcgcagctcg accatatggg cgacactttg cgccaggcgc
    13921 tgaaccaggc tttccacaac gccggtgtgg ccgggcaggc caccggcctg ggatcgttgc
    13981 tcaagctgca cctgcgcgac cagcccatca ccgattatcg cagcgcctat ccggccaagg
    14041 ccgagcggga tgccatggcc gccatcaacg tcaagctgct caaccgcggc gtgctgatgg
    14101 ccggctatgg cctgattgcc ttgtcgaccg ccatgcgcga cgcggacatt gcccacatcg
    14161 ccgaggccgc gcacgacagc ctgcgcgagt gggtcgcgga gcgcagcg
//
LOCUS       DASWPO010000171         6670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_575629, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000171 DASWPO010000000
VERSION     DASWPO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575629"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VGC69_09300"
     CDS             complement(<1..671)
                     /locus_tag="VGC69_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0295511.1"
                     /translation="MVSRLIASKFASFALAAGALAATSPAQAETISVVSFGGAYENAM
                     KEAFFKPYTAQKSNIDFNYESYDGGLAKLSAMVQAKNTTWDLMDMEEQDMIAACDEGM
                     LQPFDKSLLGDASDFLPGTIAKCGVATAMWTSVFAYDTTKLATAPTTIADFFDTRKFP
                     GKRGMRKTATLTLEAALLADGVPTTDVYKVLGTPEGLDRAFKKLDSIKSDIVWWEAGS
                     QPPQLL"
     gene            complement(725..1828)
                     /locus_tag="VGC69_09305"
     CDS             complement(725..1828)
                     /locus_tag="VGC69_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295512.1"
                     /translation="MQKEDAFIVFRSVDKTYDGSHYVVQDLSLEIARGEFLSLLGPSG
                     SGKTTTLMMLAGFETPTSGAILLGGQRLDDKPPHRRDIGMVFQNYALFPHLSIADNVA
                     FPLSVRRVGKNEQRARVKRALDMVELSHLAHRRPAQLSGGQQQRVALARALVFEPSVV
                     LMDEPLGALDKRLRETMQYEIMRLHKELSLTVVYVTHDQGEALTMSDRVAVFSDGFIQ
                     QLATPTELYENAANVFVANFVGENNGLRGNISKMDGDWAELALDGGITVRGRHGAGLR
                     LNGAATLALRPERTHLGSGDPRTENTVSGQVRKLIYCGDHHRVELKLAQGVDIIAKVP
                     NTQRQALPAAGETASVCWRHDDCKILHLPPASG"
     gene            complement(1961..2965)
                     /locus_tag="VGC69_09310"
     CDS             complement(1961..2965)
                     /locus_tag="VGC69_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY0295513.1"
                     /translation="MISAPLARYRVHLENQADLPEMFHMTAGRYQAAAQQFPALADRI
                     DLSIGWDQDNFDAAIADADVLVGWNIPRKALEQKAPRLKLLHLAGAGLDHLAPLDWIP
                     PGLTITNSSGIHAERVAEYIAMAVLALNARLPRYADDKVRRRWKPVFMPGVRGTTALV
                     VGVGGLGEAGARRCKALGMHVIGVRPSGKPSPWVDEMVGPDRLMDVLPRADVALLTMP
                     LTDKTRGLMGPRQLQAMKPGSGLINLARAGVLDHAALAQCLSAGHISGAFLDVASPEP
                     PPADHPIWSLANTIITPHISCDDPDRYIIDALRVLFENLSRLDKGDALRNTVDPARGY
                     "
     gene            complement(2962..3957)
                     /locus_tag="VGC69_09315"
     CDS             complement(2962..3957)
                     /locus_tag="VGC69_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0295514.1"
                     /translation="MLARRQIHRGSHRDSAQLPGLPQVARPANRLFLLIMTTQKTLSD
                     AAIQKFWDEGFLVMPDAISAAQLAALRNDLGGWIEESREKTGAWGELLDGRPRFDIAR
                     EHTPQNPLLRRVNNPSEISAGYRDAMENSRMTNAVAELIGPNLAFHHCKLNLKQPGAD
                     TEVGWHQDFSFTPHTNDDIVTALLFLDDVTDENGALRVSPGSHKEGQKSLWRNGKFTG
                     YVDPETEADAERRAVSATGPAGTVCFMHTSLLHGSLPNRSARSRSLYISVYRAADAFP
                     LAPSPVPSSMEGRILRGQSTHKVRLSVPEVELPDNIKTSSFFNIQAQARTTGMTM"
     gene            complement(3885..5132)
                     /locus_tag="VGC69_09320"
     CDS             complement(3885..5132)
                     /locus_tag="VGC69_09320"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0295515.1"
                     /translation="MSVEKIDTLVVGAGQSGVAMSEHLGAMGVPHIVLERQRIAERWR
                     SERWDSLVANGPAWHDRFPNLEFDDVGPDVFPPKERMARYFEDYAEMLKAPIRTGVAV
                     RSVKRNEGRPGFSVATSDGVIEAQRVVAATGPFQVPSHPDIIPADAGIDQLHSSAYRN
                     PGQLAQGAVLVVGGGASGSQIAEELRRAGRTVYLSIGEHYRPPRAYRQRDYVWWLGVL
                     GKWDEVKIKPKKKHVAFAVSGYEGGKTVDFRRLAHMGITLLGLTKSHENGVLQFEDDL
                     ARNIAEGDEAYFDVLREADAYIENNALDLPPEPEAWQLLEDPECVKRPILGLDLARAG
                     IKTVLWATGFKFDFSWLEVDAFDEHGMPLHKRGISAESGIYFLGLPELTNRSSSFIWG
                     CWHDAKYIADHIVIQRNYLAYRR"
     gene            5325..5726
                     /locus_tag="VGC69_09325"
     CDS             5325..5726
                     /locus_tag="VGC69_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0295516.1"
                     /translation="MISVDMNILPAYFHGEDATHLHLRLGTGLVSIERGRLAGGRHDG
                     GRLDIYIRPEHIRLEPISADAVLTGTVVRHVFQGDHVDIYVDIDIPASGCQRVIVCSP
                     GTDGVARWPIGTVTALALSPHDVDVFPPSAA"
     gene            5751..6380
                     /locus_tag="VGC69_09330"
     CDS             5751..6380
                     /locus_tag="VGC69_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0295517.1"
                     /translation="MQLRYAPTSPYVRKVMVAARLLGLVEKIELLDSAAHPIQRDARI
                     AAHNPLAKVPTLILADGQSLYDSRVICEYLDGLAPGYGLFPEAGPLRWRAIARQALGD
                     GLLDAALLARYEATARPPEKQWEAWRAAQRVKIEACLDAIESQAETLATRQLTIGEVT
                     LACALGYLDFRFPELDWRAARPKAAHWNAQVQALPALQATVPHTGFSPP"
     gene            6377..>6670
                     /locus_tag="VGC69_09335"
     CDS             6377..>6670
                     /locus_tag="VGC69_09335"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY0295518.1"
                     /translation="MTDTATPSSAATTVWFLNGPNANLYGLDANQVYGQESFPVLQAR
                     CEAKAAALGLTLHFVQSNWEGQLVDWIQQARGTAGGIVINAAGLTYSSVPLLDA"
BASE COUNT         1081 a         2162 c         2201 g         1226 t
ORIGIN      
        1 agcagctggg gcggctgcga gccggcttcc caccagacga tgtcgctctt gatggagtcg
       61 agcttcttga acgcgcggtc caggccttcc ggggtgccga gcaccttgta gacgtccgtc
      121 gtgggaacgc cgtcggccag cagcgccgcc tccaacgtca acgtggccgt cttgcgcatg
      181 ccgcgcttgc cggggaattt ccgggtgtcg aagaaatcgg cgatggtggt gggcgccgtg
      241 gccagcttgg tcgtgtcgta ggcgaagacg ctggtccaca tcgcggtggc cacgccgcac
      301 ttcgcgatgg tgcccggcag gaagtcggac gcgtcgccca gcaggctctt gtcgaagggc
      361 tgcagcatcc cctcgtcgca agcggcgatc atgtcctgct cttccatgtc catcaggtcc
      421 caggtggtgt tcttcgcctg gaccatcgcg gacagcttcg ccaggccgcc gtcatacgat
      481 tcgtagttga aatcgatgtt gctcttctgc gccgtgtacg gcttgaaaaa ggcctctttc
      541 atggcgtttt cataggcgcc gccaaacgaa acgacgctga tcgtttcggc ctgggccggg
      601 ctggtggcgg cgagggcgcc tgccgcaagg gcgaaggacg cgaatttcga agcaatgagg
      661 cgggaaacca tgatggcaac tcctgtggag tgaaaatgga aacagcgggg gtacggcaaa
      721 agcgtcagcc tgaagcgggc ggcagatgca ggatcttgca gtcgtcgtgc cgccagcaga
      781 cgctggcggt ttcgccggcg gcgggcagcg cctggcgctg ggtgtttgga accttcgcga
      841 tgatgtcgac gccctgcgcc agcttcagct ccacgcgatg gtggtcgccg caatagatca
      901 gtttgcgcac ctggccggac acggtgtttt ccgtccgggg gtctcccgag cccagatggg
      961 tgcgctcggg ccgcagggcc agcgtggcgg cgccgttcag gcgcaggccc gcgccgtggc
     1021 ggccgcgcac ggtgatgccg ccgtccagcg ccagctcggc ccaatcgccg tccatcttgc
     1081 tgatgttgcc ccgcaggccg ttgttctcgc caacgaagtt cgccacgaag acgttcgccg
     1141 cgttttcata gagctccgtc ggcgtggcga gctgctggat gaagccgtcg gaaaacaccg
     1201 cgacccggtc ggacatggtc aacgcctcgc cctggtcgtg cgtcacgtag acgacggtca
     1261 ggctcagttc cttgtgcagg cgcatgattt catactgcat ggtttcacgc agccgcttgt
     1321 cgagcgcgcc cagcggctca tccatcagca cgacgctggg ctcgaacacc agggcgcgcg
     1381 ccagggccac gcgctgctgc tgcccgccgg aaagctgggc cgggcggcgg tgcgccaggt
     1441 gggacagctc caccatgtcg agcgcgcgct tcacgcgcgc gcgctgctcg ttcttgccca
     1501 cgcggcgcac cgacagagga aacgccacgt tgtccgcgat gctcaggtgc ggaaacaggg
     1561 cgtagttctg gaacaccatg ccgatgtcgc gccgatgcgg cggcttgtcg tccaggcgct
     1621 ggccgccgag cagaatggcg ccgctcgtcg gcgtctcgaa cccggccagc atcatcagcg
     1681 tggtggtctt gccggaaccg gacggcccca gcagcgacag gaactctccg cgggcgattt
     1741 cgaggctcaa gtcctgcacg acgtagtgcg agccgtcgta ggtcttgtcc acgctccgga
     1801 agacgatgaa tgcgtcctct ttctgcaatg tgtttcccct ggctgccctg ccttggcgga
     1861 cagctgattt gcattatgta atgtggatca gcttaatgaa cgcggaacag ttggaaaaca
     1921 ctgttttatt gatgcgcccg ttatgttttt tcgatgcaga tcaatacccc ctggccgggt
     1981 ccaccgtgtt gcgcaacgcg tctcccttgt ccagccggga gagattctcg aacagaaccc
     2041 gcaaggcgtc gatgatatag cggtccggat cgtcgcagga gatgtgcggg gtgataatgg
     2101 tgttggccag cgaccagatc ggatggtcgg cgggcggcgg ctccgggctg gccacgtcga
     2161 gaaacgcgcc gctgatgtgg cccgcggaca ggcattgcgc gagcgccgca tgatccagga
     2221 cgccggccct ggccagattg atcaggccgc tgcccggctt catcgcctgc agctggcggg
     2281 gccccatcaa gccgcgcgtc ttgtcggtca gcggcatggt caacagcgcc acgtccgcgc
     2341 gcggcaggac gtccatcagg cgatcgggcc cgaccatttc atcgacccag gggctgggct
     2401 tgccgctcgg cctaacgccg atcacgtgca tgcccagcgc cttgcagcgc ctggcgccgg
     2461 cttcgcccag gccgcccacg cccaccacca gcgccgtggt gccgcgcacg cccggcatga
     2521 agacgggttt ccagcggcgc cggaccttgt cgtcggcgta ccggggcagc ctcgcgttca
     2581 aggccagcac cgccatcgcg atgtattcgg ccacgcgctc ggcgtggatg ccgctgctgt
     2641 tggtgatggt caagccgggc ggaatccagt ccagcggagc caggtgatcc aggcccgcgc
     2701 cggcaaggtg cagcagcttc aacctgggcg ctttctgttc aagcgccttg cgcggaatgt
     2761 tccagccgac cagcacgtcg gcatcggcaa tcgccgcatc gaagttgtcc tggtcccagc
     2821 ctatgctgag atcgatccgg tccgccaacg cgggaaactg ctgcgccgcg gcctggtagc
     2881 gtccggcggt catgtggaac atttccggca ggtcggcctg gttctccagg tgcacccggt
     2941 atcgcgccag cggagcggaa atcacatcgt catcccagtg gtccgcgcct gcgcttgaat
     3001 gttgaagaac gaactggtct tgatgttgtc gggcagttcg acttcgggca cggacagccg
     3061 gaccttgtga gtcgattggc cgcgcaggat gcggccttcc atcgagctgg gaacgggcga
     3121 aggcgccagg gggaaggcgt ccgcggcgcg gtagaccgag atgtagagac tgcggctcct
     3181 ggcgctgcga ttcggcagcg aaccgtgcag cagggatgtg tgcatgaagc agacggttcc
     3241 cgccgggccg gttgccgaga cggcgcgccg ttccgcgtcg gcctcggtct cggggtccac
     3301 gtagcccgtg aacttgccgt tgcgccaaag gcttttctgg ccttccttgt gggaaccggg
     3361 ggacacgcgc agcgcgccgt tctcatcggt cacgtcgtcc aggaacagca gcgccgtgac
     3421 gatgtcgtca ttggtgtgcg gcgtgaatga aaaatcctgg tgccagccca cttccgtgtc
     3481 ggcgccaggc tgtttgaggt tcagcttgca gtggtggaac gccaggttcg gcccgatcaa
     3541 ctccgcgacg gcattcgtca tgcggctgtt ttccattgcg tcccgatagc cggccgatat
     3601 ttccgaagga ttgtttacgc ggcgcagcag tggattctgc ggcgtgtgct cgcgcgcgat
     3661 atcgaaccgg ggacggccgt ccagcagctc gccccatgcg cccgtttttt cccggctttc
     3721 ctcgatccag ccgcctaggt cattgcgcag cgcggccaat tgcgccgcgc tgatggcatc
     3781 gggcatcaca agaaaacctt cgtcccagaa tttctggatt gccgcgtcag agagtgtttt
     3841 ttgcgtagtc atgatgagga ggaatagtcg attggcgggc cgggctacct gcggtaggcc
     3901 aggtaattgc gctgaatcac gatgtgatcc gcgatgtatt tggcgtcgtg ccagcatccc
     3961 cagatgaaag acgatgaccg gtttgtcaac tccggcaggc ccaggaagta aataccgctt
     4021 tccgcggata tcccccgctt gtgcaagggc atgccgtgct cgtcgaacgc gtcgacttcc
     4081 agccagctga aatcgaactt gaaacccgtc gcccacagga cggtcttgat cccggcccgc
     4141 gcgaggtcga ggccgagaat cggccgcttc acgcattcgg ggtcttcgag aagctgccag
     4201 gcctcgggtt cgggcggcag gtccagggcg ttgttctcga tgtaggcatc ggcctcgcgc
     4261 agcacgtcga agtaggcttc atcgccttcg gcgatgttcc tggccaggtc gtcctcgaat
     4321 tgaagcaccc cgttttcatg ggacttggtc agcccgagca gcgtgatgcc catgtgggcc
     4381 aggcgccgga agtccaccgt cttgccgccc tcgtagccgc tcacggcgaa ggcgacgtgc
     4441 ttcttcttcg gcttgatctt gacttcgtcc cacttgccga gcacgcccag ccaccacacg
     4501 tagtcgcgct ggcgataggc gcgcggcggg cggtagtgct cgccgatgga gagatagacg
     4561 gtccggccgg cgcggcgcag ctcttcggcg atctgcgacc ccgaggcgcc gccgcccacg
     4621 accagcaccg cgccctgggc caattgtccg gggttgcggt aggccgagga atgcagctgg
     4681 tcgatgccgg cgtcggcggg aatgatgtcc gggtggctgg gcacctggaa cgggccggtg
     4741 gccgccacga cgcgctgggc ctcgatcacg ccgtcggacg tggcgacgct gaagccgggc
     4801 ctgccttcgt tgcgcttgac gctcctgacc gcgacgccgg tgcggatcgg agccttgagc
     4861 atctcggcgt agtcttcgaa gtacctggcc atgcgttcct tgggcggaaa gacgtcggga
     4921 ccgacgtcgt cgaactccag gttgggaaag cggtcgtgcc aggccgggcc gttggcgacc
     4981 agggaatccc agcgttccga gcgccagcgc tccgcgatgc gctggcgttc gagcacgatg
     5041 tgcggaacgc ccatcgcgcc caggtgttcg ctcatcgcca ccccggactg accggccccg
     5101 acgacgagcg tatcgatttt ttcaactgac atctttggtt cccaagaaaa gcccatggcc
     5161 gcaagtgtgc tcaagcccgc cgcgctcgag aatcatgatt tttggactcg gggcatcgga
     5221 aaaagtaaac gcagacgccg tccgggcgaa ttctgttttt ctgacgcgag ggctcaagat
     5281 tcgcaaagcc gaggagggcc gcggctgccg tattcttcga gaccatgatc tccgtcgaca
     5341 tgaacatcct gccggcgtac ttccacggcg aagatgcgac gcacctccac ctgcggctgg
     5401 gcacgggcct ggtcagcatc gagcggggcc gcctggccgg cggccggcac gacggcgggc
     5461 ggttggacat ctacatccgc ccggaacaca tccgcctgga gccgataagc gcggacgcgg
     5521 tcctgaccgg caccgtggtg cgccatgtat tccagggcga tcacgtcgat atctacgtgg
     5581 acatcgacat tcccgccagc ggatgccagc gcgtgatagt gtgttcgccg ggcacggacg
     5641 gcgtggcccg ctggcccatc ggcaccgtga ccgcattggc gctgtcgccg catgacgtgg
     5701 atgtctttcc gccgtcggcg gcctgaccga tgacgccgcc gacttccatg atgcaactac
     5761 gctacgctcc cacttccccc tatgtgcgca aggtgatggt cgccgcccgc ctgctgggcc
     5821 tggtcgagaa aatcgaactg ctcgacagcg ccgcccaccc catccagcgc gatgcgcgca
     5881 tcgcggcgca caatcctttg gccaaggtgc cgaccctgat cctggccgac ggccagtcgc
     5941 tgtacgacag ccgcgtcatc tgtgaatatc tcgacggcct tgcgcccggc tatggcttgt
     6001 tccccgaggc cggccccttg cgctggcggg cgattgcccg ccaggcgctg ggcgacggcc
     6061 tgctcgacgc ggccctgctg gcgcgctacg aggctaccgc ccgcccgccg gaaaagcagt
     6121 gggaggcctg gcgtgccgcg caacgggtca aaatcgaggc ctgcctcgat gccatcgaga
     6181 gccaggccga gacgctggcg acgcgccagc tgaccatcgg cgaagtcacg ctcgcctgcg
     6241 cgcttggcta cctggatttc cgctttcccg aactcgattg gcgcgccgcg cgtcccaagg
     6301 ccgcgcactg gaacgcccag gtccaggcgc tgcccgcgct gcaagccact gttccccaca
     6361 ctggattttc gccgccatga ccgacaccgc cacgccttcg tccgccgcca cgaccgtctg
     6421 gttcctcaat ggtcccaacg ccaacctgta cgggctcgac gccaaccagg tctacgggca
     6481 ggagagcttc cccgtattgc aggcgcgctg cgaggccaag gctgcggcgc tgggcctgac
     6541 gctgcatttc gtccagtcga actgggaagg gcagttggtg gactggatac agcaggcgcg
     6601 gggcacggcc ggcggcatcg tcatcaacgc cgccggcctg acgtattcct cggtgccgct
     6661 gctcgatgcc
//
LOCUS       DASWPO010000172         9164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1469046, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000172 DASWPO010000000
VERSION     DASWPO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469046"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..656)
                     /gene="dksA"
                     /locus_tag="VGC69_09340"
     CDS             complement(198..656)
                     /gene="dksA"
                     /locus_tag="VGC69_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY0295519.1"
                     /translation="MATKAASKKSSKSEATIDLPSEKELLAMPESDYMNDRQLAFFKE
                     RLKQLEQDILANAGETTEHLRETQFVPDPADRATIEEEHALELRTRDRERKLLKKVQQ
                     AIARIDSGEYGWCEETGEPIGIPRLLARPTATLSLEAQERREMRQKLYGD"
     gene            complement(727..1791)
                     /locus_tag="VGC69_09345"
     CDS             complement(727..1791)
                     /locus_tag="VGC69_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0295520.1"
                     /translation="MNTPLASMVPVTILTGFLGAGKTTLLKRILSEYHGRRLAVIENE
                     FGPESIDNDLLVQDSDEEIIELSNGCVCCTVRGDLMRTLNDLRAKRQAGKLNFERVII
                     ETTGMANPGPVCQTFFMDDDIADYYRLDAVITVVDAKHGQETLDVQTEAQKQVGFADR
                     ILVSKKDLVSEDEYAALRERLVRMNPRASITPVHFGEADLKSVIDISGFNLNSILDID
                     PEFLADEHPDAAHDHDHDDHDHGHGEHDHHHHHHHHDDEIGAFVFRSNKPFDPARLEE
                     FLGGVVQVYGPDLLRYKGILYMKGLNRRMLFQGVHMMMGAEPGKPWTSAEKPSTKMVF
                     IGRKLPQEIFTRGLEQCLAG"
     gene            complement(2005..2514)
                     /locus_tag="VGC69_09350"
     CDS             complement(2005..2514)
                     /locus_tag="VGC69_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY0295521.1"
                     /translation="MSDSPTDAHDIEAQLALADTLCAQRGKRLTPIRRKVLELLLRHG
                     RSIKAYELLDAIKAEHPGAAPPTVYRALDFLVEEGLIHRLDAVNAWSACHDAAGDPHD
                     LLVVCTHCGAVAEISDPAMSRLLAERVAKTGFVLATHETEIRALCPDCQKDQPKDAGH
                     GHTHAGHAH"
     gene            complement(2524..3504)
                     /gene="xerC"
                     /locus_tag="VGC69_09355"
     CDS             complement(2524..3504)
                     /gene="xerC"
                     /locus_tag="VGC69_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY0295522.1"
                     /translation="MTEPAAPLNAPMQAWLQHLQANRRYAGHTLDGYGRDLRRLAELA
                     TQAKVPLERIANGHIRQFVARLHAQGLGPRSLARALAAWRGFYQWWAPQAGLPGNPAA
                     GVRAPKAPRGLPKALSVEQAQGLLDHANTQAGLQGAGDPAALRDAAMFELFYSSGLRL
                     SELIGLDWRYLREDGYESRSWFNRGEAEVTVLGKGGKRRSVPVGQAALAAIDRWLPVR
                     NAMLAPAAAPGDQAALFLGARGKRIAPRVVQMRLAALAQAAGLPAHVHPHVLRHSFAS
                     HVLQSAQDLRAVQEMLGHANITTTQIYTRLDFQHLARAYDQAHPRAGRKS"
     gene            complement(3527..4195)
                     /locus_tag="VGC69_09360"
     CDS             complement(3527..4195)
                     /locus_tag="VGC69_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY0295523.1"
                     /translation="MTYSAQDIADFLAQHPAFFEEHADVFTAMRVPHPHSGQTISLAE
                     RQILTLRERGREQEWRINELVRNAVANETITTRLTQWCEGLVGEANPQHLPGAIALGL
                     GEQFKIEHVALRLWSLPGLAADSGYAAGVSEDVRTFADSLKTPYCGNDTEFEAAAWLD
                     AKPKSLALIALRPAEDEASIGLLALGSDDPARFDPDMGTVFLDTIARLSCAALRRLAE
                     PAAG"
     gene            complement(4218..5129)
                     /gene="dapF"
                     /locus_tag="VGC69_09365"
     CDS             complement(4218..5129)
                     /gene="dapF"
                     /locus_tag="VGC69_09365"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0295524.1"
                     /translation="MRDNRTMIWNFTKMHGAGNDFVVLDGVRQTLDMTPERARALADR
                     HFGIGADQILLVEPATRPDADFRYRIFNADGSEVEHCGNGARCFVRFVHEQGLSDRNP
                     LRAEIMTGVIVLDEGDDAQVTVDMGSTRFDPESLPFDTAGLASRTQHQDTLWTLAYDA
                     PAGIAGSADISVVAISNPHAVQVVDDVDAAPVAVVGPLIESHKRFPRRVNAGFMQVLG
                     PHDIKLRVYERGAGETLACGTGACAAVAAGVRRGLLQSPVRVQTRGGVLIIAFDGRQL
                     RMTGPAESVFNGALDIDKLVFSLALNR"
     gene            complement(5317..6213)
                     /locus_tag="VGC69_09370"
     CDS             complement(5317..6213)
                     /locus_tag="VGC69_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0295525.1"
                     /translation="MDKFKTWLLVGLLRWFARMGHPARQRAGRVAAWLAFRLAGRRVR
                     IARRNLELCFPEASAQTRERWVREHFRALGQSIVDRGVLWYGSPDAIRDMVSLEGCEY
                     ITDTLKQGKPVLILAPHFITLDATGTRLTMEMTGKYPEGASMYTPQRDPVIDAVTRRG
                     RARFNTIHLVSRHNGVRELVRHLRNGLPVYYLPDMDFGAEGSAFVPFFGIPTATLTAT
                     AQLARKAAVVPAIGYWNPATGRYHIEVHPPLADFPGAGTLEEATARLNRELETWIRRC
                     PSQYYWVHRRFKTRPEGEPSLY"
     gene            complement(6221..7084)
                     /locus_tag="VGC69_09375"
     CDS             complement(6221..7084)
                     /locus_tag="VGC69_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0295526.1"
                     /translation="MLLILVRLLAALPLPVLHTLGRLAGRLVYACPSRYRRRLRANAA
                     QAGYPDAAFARRAAGEIGAMILETPRIWFRHEASLAMVVSDDEQLVQQARAEGKGLLF
                     LTPHLGSFEICARYITRDIPGTVMFRPPRKAILAPLFDSARNTANVHAVPATQQGVRE
                     FVRALRRGELVGMLPDQVPGPSEGVWAPFFGRMAYTITLPVKLAVQTGATIIVTTGER
                     LPGGRGWRMHYVRVPAPLPEDATAQAALLNEAMEALIRRFPHQYLWGYNRYKTPPGAP
                     PAPDADAFPST"
     gene            7377..8558
                     /gene="metK"
                     /locus_tag="VGC69_09380"
     CDS             7377..8558
                     /gene="metK"
                     /locus_tag="VGC69_09380"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY0295527.1"
                     /translation="MANDFLFTSESVSEGHPDKVADQISDAILDAIFEQDPKSRVAAE
                     TLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYDNTDYGIDYRGCAVLVAYDKQSN
                     DIAQGVDHASDDYLNTGAGDQGLMFGYACDETPDLMPAPIYYAHRLVERQAQLRKDGR
                     LPFLRPDAKSQITMRYVDGKPHSIDTVVLSTQHSPDQSETQTKMKASFIEAVIEEIIK
                     PVLPKEWINDTRYLINPTGRFVVGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDP
                     SKVDRSAAYAARYVAKNVVAAGLAKQCQIQLSYAIGVAEPINITVYTEGTGIISDDQI
                     AKLVREHFDLRPKGIINMLDLLRPIYAKTAAYGHFGRPEPEFTWEATDKAQALKAAVG
                     K"
     gene            8682..>9164
                     /locus_tag="VGC69_09385"
     CDS             8682..>9164
                     /locus_tag="VGC69_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0295528.1"
                     /translation="MHNLFDLPLRRIEANGIRLAARIGGEGPPLLLLHGHPQTHAIWH
                     KVWPGLARRHTCVAVDLRGYGDSDKPAASPGHAAHAKRAMAQDMADLMRGLGHARFAV
                     LAHDRGARVAHRLALDHADRVTRMMLLDIAPTLDMYEGTTRAFAQAYFHWFWLIQPAP
                     L"
BASE COUNT         1395 a         2883 c         3385 g         1501 t
ORIGIN      
        1 ccggaccgtt gaggcggggg cagccccgta ggggccgagg gcccgtcatc ttgggcccga
       61 gccccgccga tggtccggcg accccccacc cccgcctgat ggatggccca agaactcttg
      121 ttttacccgc ccaaaaacaa aaaggccacg cataaaaagc gtggccttcc ggggacagag
      181 cgcagaaaaa gtgccgttta gtcgccgtac agcttctggc gcatctcgcg gcgctcctgc
      241 gcctcgagcg acagggtggc ggtggggcgg gccagcaggc ggggaatgcc gatgggctcg
      301 ccggtttcct cgcaccagcc gtattcgccg ctgtcgatgc gggcgatggc ctgctgcacc
      361 ttcttgagca gcttgcgttc gcggtcgcgg gtgcgcagtt cgagcgcgtg ctcttcctcg
      421 atggtggcgc ggtcggccgg atcgggcacg aactgggtct cgcgcaagtg ctcagtggtt
      481 tcgccggcgt tggccaggat gtcctgctcg agctgcttga gccgttcttt gaagaaagcc
      541 agctggcgat cgttcatgta gtcggactcg ggcatggcca gcaattcttt ttcgctgggc
      601 agatcgatcg tcgcttccga cttgcttgat tttttgcttg ctgccttcgt tgccatgaaa
      661 atcactccac tatgaatcga tccgggcgcg catgccgcgc gccggtgagc cggccgggct
      721 cagcgcttaa ccggccaggc actgctccag gccccgggtg aaaatttcct ggggcagttt
      781 gcgtccaatg aacaccatct tggtcgaggg cttctcggcc gacgtccagg gcttgccggg
      841 ttcggcgccc atcatcatgt gcacgccctg aaaaagcatg cggcggttga ggcccttcat
      901 gtaaaggatg cctttgtagc gcagcaggtc ggggccgtaa acctggacaa ccccaccgag
      961 gaattcttcg aggcgggccg ggtcgaaagg cttgttcgag cggaacacga aggcaccgat
     1021 ctcgtcatcg tgatgatgat ggtggtgatg gtggtcgtgc tcgccgtgcc cgtgatcatg
     1081 gtcgtcgtgg tcatgatcgt gcgcggcgtc ggggtgttcg tcggccagga attccgggtc
     1141 gatgtccagg atggagttca ggttgaagcc gctgatgtcg atgacggact tgaggtcggc
     1201 ctcgccgaaa tgcaccggcg tgatcgaggc gcgcgggttc atgcgcacca ggcgctcgcg
     1261 cagggcggcg tattcgtcct cggacaccag gtccttcttg gacaccagga tgcggtcggc
     1321 aaagcccacc tgcttctgcg cctcggtctg gacgtcgagc gtttcctggc cgtgcttggc
     1381 gtcgaccacc gtgatcaccg cgtcgaggcg gtagtagtcg gcgatgtcgt cgtccatgaa
     1441 gaaggtctgg cacaccgggc cggggttggc catgccggtg gtctcgatga tgacgcgctc
     1501 gaagttgagc ttgccggcct ggcgcttggc gcgcaggtcg ttgagcgtgc gcatcaggtc
     1561 gccgcgcacc gtgcagcaga cgcagccgtt ggacagctcg atgatttcct cgtcgctgtc
     1621 ctgcaccagc aggtcgttgt cgatgctctc gggtccgaat tcgttttcga tcacggcgag
     1681 gcgccggccg tggtattcgg acaggatgcg tttgagcagg gtcgttttgc ctgcgccgag
     1741 aaagccggtg aggatggtga cgggaaccat ggaagctaag ggggtattca tgatggctga
     1801 gaacgccgga aaacgctgga ttacagcata gtcgcaagcc aggggcaaac ctggggaaga
     1861 caacctaggt gggggcttgc ggccggattt caagggccgg ggccggcttg ctacggccct
     1921 attttgccgc cggcccgtcc caaggcaaga aaatgctcct tgggagccgg cacgcagtgc
     1981 gcggcgcggg gagcgctttc ctgctcagtg ggcgtgcccg gcgtgggtgt ggccgtgccc
     2041 ggcatctttc ggctggtctt tctggcagtc ggggcagagg gcgcggatct cggtttcgtg
     2101 ggtggccagg acgaagccgg tcttggccac gcgttcggcc agcaggcggc tcatggccgg
     2161 atcgctgatt tcggcgacgg cgccgcagtg ggtgcagacc accagcaggt cgtgcggatc
     2221 gccggcggca tcgtggcagg cactccaggc gttgacggcg tccaggcggt ggatgaggcc
     2281 ttcctcgacc aggaagtcca gggcccggta gacggtaggg ggcgcggcgc cgggatgttc
     2341 ggccttgatg gcgtcgagca gctcgtaggc cttgatgctg cggccgtggc gcagcaacag
     2401 ctccagcacc ttgcggcgga tgggcgtgag ccgcttgccg cgctgggcgc agagcgtgtc
     2461 ggccagcgcg agctgggcct cgatgtcatg ggcgtcggtc gggctgtcgg acatgggcgg
     2521 gcttcaggat ttgcggccgg cccgcgggtg ggcctggtcg taggcacggg ccaggtgctg
     2581 gaaatcgagc cgtgtgtaga tctgggtagt cgtgatattg gcatgtccca gcatttcctg
     2641 cacggcgcgc aggtcttgcg ccgattgcag cacgtggctg gcgaagctgt ggcgcagcac
     2701 gtgcgggtgc acgtgggccg gcaggccggc ggcctgggcc agggcggcca ggcgcatctg
     2761 cactacgcgc ggggcgatgc gcttgccgcg cgcgcccagg aacagggcgg cctggtcgcc
     2821 gggggcggcg gcgggcgcca gcatggcgtt gcgcaccggc agccagcggt cgatggccgc
     2881 gagcgcggcc tggcccaccg gcacgctgcg gcgcttgccg cccttgccca gcacggtgac
     2941 ctcggcttcg ccgcggttga accagctgcg cgattcgtag ccgtcttcgc gcaggtagcg
     3001 ccagtccagc ccgatgagtt cggacaggcg cagcccgctg gaatagaaca gctcgaacat
     3061 ggccgcatcg cgcagggccg cggggtcgcc cgcgccctgc aagccggctt gggtgttggc
     3121 atggtcgagc agcccctggg cctgttccac ggagagcgcc ttgggcaggc cgcgcggcgc
     3181 cttgggcgcg cgcacgccgg ccgccgggtt gccgggcagc ccggcctgcg gcgcccacca
     3241 ttggtagaag ccgcgccagg cggccagggc gcgggccagg ctgcgcgggc ccaggccctg
     3301 ggcatgcagg cgggcgacga actggcggat gtggccgttg gcgatgcgct cgagcggcac
     3361 cttggcctgt gtggccagct cggccaggcg gcgcaggtcg cggccgtagc cgtccagggt
     3421 gtggccggcg tagcgtcggt tggcctgcag gtgctgcagc caggcctgca tgggggcgtt
     3481 gagcggggcg gcaggctcgg tcatggcagg ctcgggtggg cggcggtcag ccggccgcgg
     3541 gttcggccag gcggcgcagc gcggcgcagg acaggcgggc gatggtgtcg agaaacaccg
     3601 tgcccatgtc ggggtcgaag cgcgccgggt cgtccgagcc cagcgccagc aggccgatgc
     3661 tggcctcgtc ttcggccggg cgcagcgcga tcagggccag cgacttgggc ttggcgtcga
     3721 gccaggcggc ggcttcgaat tcggtgtcgt tgccgcagta gggggtcttg aggctgtcgg
     3781 cgaaggtgcg cacgtcttcg gaaacgcccg cggcatagcc gctgtcggcc gccaggccgg
     3841 gcaggctcca caggcgcagc gccacgtgct cgatcttgaa ttgctcgccc aggcccaggg
     3901 cgatggcgcc gggcaggtgc tgcggattgg cttccccgac gaggccttcg caccattgcg
     3961 tgaggcgggt ggtgatggtt tcgttggcga cggcgttgcg cacgagttcg ttgatgcgcc
     4021 attcctgttc gcggccgcgt tcgcgcagcg tgaggatctg ccgttcggcc agcgagatgg
     4081 tctgcccgga atgcggatgc ggcacgcgca tggcggtgaa cacatcggcg tgttcctcga
     4141 agaaagccgg atgctgagcg aggaagtcgg cgatgtcttg ggcggagtag gtcatggcgt
     4201 cgggaagggg cggcgtgtca gcggttgaga gccagggaga aaaccagctt gtcgatgtcg
     4261 agggcgccgt tgaagacgga ttcggccggg ccggtcatgc gcagctggcg gccgtcgaag
     4321 gcaatgatga ggacgccgcc gcgcgtctgc acgcgcaccg ggctttgcag gaggccgcgg
     4381 cgcacgccgg ccgcgacggc cgcgcaggcg ccggtgccgc aggcgagcgt ttcgcccgcg
     4441 ccgcgttcat agacgcgcag cttgatgtcg tgcgggccga gcacctgcat gaagccggcg
     4501 ttgacgcgcc tggggaagcg cttgtgcgat tcgatcagcg ggccgaccac ggccacgggc
     4561 gcggcgtcga cgtcgtcgac gacctgcacg gcatggggat tggagatggc gacgacggag
     4621 atgtcggcgc tgcccgcgat gccggcgggg gcgtcgtagg ccagggtcca gagcgtgtct
     4681 tggtgctggg tgcgcgaggc caggccggcg gtgtcgaagg gcagggactc gggatcgaag
     4741 cgggtgctgc ccatgtcgac ggtgacctgg gcgtcgtcgc cctcgtcgag cacgatgacg
     4801 ccggtcatga tctcggcgcg cagggggttg cggtcggaga ggccctgctc gtggacgaag
     4861 cgcacgaagc agcgcgcgcc gttgccgcag tgctcgacct cgctgccgtc ggcgttgaag
     4921 atgcggtagc ggaagtcggc gtcggggcgc gtggcgggtt cgaccagcag gatctggtcg
     4981 gcgccgatgc cgaaatgacg gtcggccagg gcgcgggcgc gctcgggcgt catgtccagg
     5041 gtttggcgca cgccgtcgag gacgacgaag tcgttgccgg cgccgtgcat tttggtgaag
     5101 ttccagatca ttgttcgatt atcgcgcaaa ctcattgggt tcttgagacg cagcgtcgcc
     5161 tcgggcctgg gagggggatc ccggcccgat cggcagggct cgggcgcaag acgacgcgcc
     5221 ctcggccccc ttcgggggct gcccccgcct caaagggccg ggatccccct cccaggcccg
     5281 aggcgacgct gctcgcgtag tttcacaacc gtagtctcaa tacaacgatg gttcgccttc
     5341 cggccgggtc ttgaagcggc ggtgcaccca gtagtactgg ctggggcagc ggcggatcca
     5401 ggtttcgagt tcccggttca ggcgggcggt ggcttcctcc agcgtgcctg cgccggggaa
     5461 gtcggccagc gggggatgca cttcgatatg gtagcggccg gtggcggggt tccaatagcc
     5521 gatggcgggg acgacggcgg ccttgcgggc cagttgggcg gtcgccgtca gcgtggcggt
     5581 ggggatgccg aaaaagggca cgaaggccga gccttcggcg ccgaagtcca tgtcgggcag
     5641 gtagtacacg ggcaggccgt tgcgcaggtg gcgcaccagt tcgcgcacgc cgttgtggcg
     5701 gctcaccagg tggatggtgt tgaagcgcgc ccggccccgg cgcgtgacgg cgtcgatcac
     5761 ggggtcgcgc tgcggggtgt acatggaggc cccctcgggg tatttgccgg tcatctccat
     5821 ggtcaggcgc gtgccggtgg catccagcgt gatgaaatgc ggcgccagga tgagcaccgg
     5881 cttgccctgc ttgagcgtgt cggtgatgta ttcgcagcct tccaggctga ccatgtcgcg
     5941 gatggcgtcg gggctgccgt accagagcac gccgcggtcg acgatggact ggcccagggc
     6001 gcggaagtgc tcgcggaccc agcgttcgcg ggtctgcgcg gacgcttcgg gaaagcacag
     6061 ttcaaggttg cgccgcgcga tgcgcacgcg ccgcccggcc agccggaacg ccagccaggc
     6121 ggcgacgcgg ccggcgcgtt ggcgcgcggg gtgacccatg cgggcgaacc agcgcagcaa
     6181 tccaaccagt agccaggtct tgaatttgtc catcggtgat tcaggtggag gggaaggcgt
     6241 ccgcatcggg cgccggcggc gcgccgggcg gcgtcttgta gcggttgtag ccccacaggt
     6301 attggtgggg gaaccggcgg atgagggctt ccatcgcctc gttgaggagc gcggcctggg
     6361 ccgtggcgtc ttcgggcagg ggggctggaa cgcgcacata gtgcatgcgc cagccgcgcc
     6421 cgccgggcag gcgctcgccg gttgtcacga tgatggtcgc gccggtctgc acggccagct
     6481 tgacgggcag ggtgatggtg taggccatgc ggccgaagaa gggagcccag acgccttcgc
     6541 tcgggccggg gacctggtcg ggcagcatcc ccaccagttc gccgcggcgc aaggcgcgca
     6601 cgaattcgcg caccccctgc tgcgtggcgg gcacggcatg cacgttggcg gtgttgcggg
     6661 cgctatcgaa caaaggggcc aggatggcct tgcgaggcgg gcggaacatg accgtgccgg
     6721 ggatgtcgcg ggtgatgtag cgcgcgcaga tctcgaagct gcccaggtgc ggcgtgagga
     6781 acagcaggcc cttgccttcg gcgcgggcct gctgcacgag ttgctcgtcg tcggacacga
     6841 ccatggccag gctggcttcg tggcggaacc agatccgggg ggtttccagg atcatggcgc
     6901 cgatctcgcc ggcggcgcgg cgggcgaagg ccgcgtcggg atagccggcc tgggcggcat
     6961 tggcccgcag gcgccggcgg tacctgctgg ggcaggcgta gaccagccgg ccggccaggc
     7021 ggccaagcgt gtgcagcacg ggcaggggca gggcggcgag cagccggacc aggatgagca
     7081 gcatgaaggc ggcgatagga agaaagttgc aatgcttatg cggccgggcc ggagtaggcg
     7141 agggcttacc ggggccggat ctgcaagacg acggtatttt cgcctagaat agggccaagt
     7201 cgccgagtta accgacaact tgcggagcga cggcggcctg ggatgcactc cgggccggcc
     7261 aaatccgcta aagcgtcgcg cccagaagtc accctggagc cgggcttgta caaggccact
     7321 cgaggttggg gttcaggggc gcagcgcgcc tctgtttcaa cctaaaggac atttctgtgg
     7381 ctaacgattt tctcttcact tccgaatccg tctccgaagg ccatcccgac aaggtcgccg
     7441 atcagatttc cgacgcgatc ctggacgcca tcttcgagca ggaccccaag tcccgcgttg
     7501 ccgccgagac gctgaccaac accggtctgg tggtgctcgc cggtgaaatc accaccaatg
     7561 cgcacgtcga ctacatccag gtcgcgcgcg acaccatcaa gcgcatcggc tacgacaaca
     7621 ccgactacgg catcgactac cggggttgcg cggtgctggt ggcctacgac aagcagtcca
     7681 acgacatcgc ccagggcgtg gaccacgcca gcgacgacta cctgaacacc ggcgccggcg
     7741 accagggcct gatgttcggc tacgcctgcg acgagacccc cgacctgatg cccgcgccga
     7801 tctactacgc gcaccgcctg gtcgagcgcc aggcccagct gcgcaaggac ggccgcctgc
     7861 ccttcctgcg ccccgacgcc aagtcgcaga tcaccatgcg ctacgtcgac ggcaagccgc
     7921 acagcatcga caccgtcgtg ctgtcgaccc agcacagccc cgaccagtcc gaaacgcaga
     7981 ccaagatgaa ggccagcttc atcgaggccg tgatcgaaga gatcatcaag cccgtgctgc
     8041 ccaaggagtg gatcaacgac acgcgctacc tgatcaaccc caccggccgc ttcgtcgtgg
     8101 gcggcccgca gggcgactgc ggcctgaccg gccgcaagat catcgtcgac acctacggcg
     8161 gcgcctgccc gcacggcggc ggcgcgttct cgggcaagga cccctccaag gtcgaccgct
     8221 cggccgccta cgcggcgcgc tacgtggcca agaacgtggt ggccgcgggc ctggccaagc
     8281 agtgccagat ccagctgtcc tacgccatcg gcgtggccga gcccatcaac atcacggtct
     8341 acaccgaagg cacgggcatc atttccgatg accagatcgc caagctggtg cgcgagcatt
     8401 tcgacctgcg tcccaagggc atcatcaaca tgctcgacct gctgcgcccc atctacgcca
     8461 agacggccgc ctatggccac ttcggccgcc ccgagcccga gttcacgtgg gaagccacgg
     8521 acaaggcgca ggcactgaag gccgccgtgg gcaagtaagc cgccggcagg ctggcagcct
     8581 gcccgtcccg ccccgcaaac ccccggcatg ccgggggttt tttcctgcgc gccgtccggg
     8641 cctgcgcggg ctacgatgag ggccttgcct gggagggttt catgcacaat ctgttcgacc
     8701 tgccgctgcg ccgcatcgag gccaacggca ttcgcctggc cgcgcgcatc gggggcgaag
     8761 ggccgcccct gctgctcctg cacgggcatc cgcagacgca tgcgatctgg cacaaggtct
     8821 ggcccgggct ggcgcgccgg cacacctgcg tggcggtcga cctgcgcggc tacggcgaca
     8881 gcgacaagcc ggcggcctcg cccggccatg ccgcccacgc caagcgcgcc atggcgcaag
     8941 acatggccga cctgatgcgc ggcctgggcc acgcgcgctt cgccgtgctg gcgcacgatc
     9001 gcggcgcgcg ggtggcgcat cgcctggccc tggatcacgc cgaccgggtg acgcgcatga
     9061 tgctgctgga catcgcgccg accctggaca tgtacgaggg caccacgcgc gccttcgccc
     9121 aggcgtattt ccactggttc tggctgatcc agcccgcgcc cctg
//
LOCUS       DASWPO010000173         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_486730, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000173 DASWPO010000000
VERSION     DASWPO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486730"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGC69_09390"
     CDS             complement(<1..435)
                     /locus_tag="VGC69_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HEY0295529.1"
                     /translation="MSTESNSKGSRSRSKSSSARGASSGFSLRNVRVGTLLLLVMALF
                     FVLVASVGGMAAYFLQENYKSVQAVGELTARAREVATINSDMLRARVGLMVAARYYQE
                     AGWGGGDAATASAKKSMDEAVATLDGVRSHFNDFQKNMLEDDA"
     gene            complement(527..946)
                     /locus_tag="VGC69_09395"
     CDS             complement(527..946)
                     /locus_tag="VGC69_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295530.1"
                     /translation="MLLVLAGCATHGHNFDSSKLSALTPGQTTLAQAAYVLEAPPDNI
                     YPQSDGTTLAFWQFHISFLNDGLYSRKEAMLQFGPDGRLMRLVDSTNLLLEPWERRKL
                     LGVAPPPAAQPAPAVAAGADSAGPQTYAIPVPAASAP"
     gene            complement(1004..>1699)
                     /locus_tag="VGC69_09400"
     CDS             complement(1004..>1699)
                     /locus_tag="VGC69_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0295531.1"
                     /translation="AFQTNILALNAAVEAARAGEQGKGFAVVAGEVRSLAQRSAQAAK
                     EIKVLIEDSVGKVNVGSEQVERAGNTMQEIVTSVKRVTDIMGEISAASEEQSGGIDQV
                     NRAVSQMDEVTQQNAALVEQAAAAAGSLEEQARHLAEAVSVFKINSGEVIDVQARQLA
                     SPRAAATRAVTSPTAQPRVAAAPSAAPRLPSAKPPVGGKPVAKKPSAAPAAPAAPRAR
                     KAVAAGSDDWESF"
BASE COUNT          271 a          613 c          520 g          295 t
ORIGIN      
        1 ggcatcgtcc tccagcatgt ttttctggaa atcgttgaag tggctgcgca cgccgtcgag
       61 cgtggccacc gcctcgtcca tggacttctt cgcggaagcc gtggcggcat cgccaccacc
      121 ccagccggcc tcctggtaat agcgcgcggc caccatcaac cccacccgtg cccgcagcat
      181 gtcgctgttg atggtggcca cttcgcgcgc ccgggcggtc agctcgccga ccgcctgcac
      241 cgacttgtag ttctcctgca ggaaataggc ggccatgccg cccacgcttg ccaccagcac
      301 gaaaaacaag gccatgacca gcaacagcag ggttcccacg cgcacattgc gcaggctgaa
      361 acccgacgac gcgccccggg cggaggatga cttgctgcgg gacctggatc ccttgctgtt
      421 cgattctgta ctcataatgg ctcttccctc tctccgtacg gttcataggc cgcactgttt
      481 taaaacacga ctcgtccttg catgcgatgc catgcaacgc gaacgactat ggcgcggacg
      541 ctgccggaac tggaattgcg taagtctgcg gtccggccga atccgcgcct gcggccacgg
      601 ccggcgcagg ctgggcggca ggcggcggcg ccacgcccag cagtttccgc cgctcccaag
      661 gctcgagcag caggttggta ctgtcgacca ggcgcatcaa gcgtccgtcc gggccgaact
      721 gcagcatcgc ctccttgcgg ctgtacaagc catcgttcag gaagctgatg tggaactgcc
      781 aaaacgccag cgtcgtgccg tcgctttgcg gatagatatt gtccggcggg gcctccagca
      841 cataggccgc ctgcgccagg gtggtctgcc ccggcgtcag cgccgagagc ttgctggaat
      901 caaaattgtg cccatgggtg gcgcaccccg ccagcaccag gagcattgcg gcggcgaacg
      961 ccttcaaacc ctgcatgacc ctacctcgaa acatcaaaaa cgtttagaag gattcccaat
     1021 catccgaacc cgccgctacc gccttgcggg cgcgcggggc tgccggcgcc gccggcgcgg
     1081 cggacggctt cttggccacg ggcttgccgc ccacgggcgg cttggccgac ggcaggcgcg
     1141 gcgcggcgga cggcgcggcg gccacccgcg gctgggcagt tggcgaagtc acggcgcgcg
     1201 tcgccgccgc gcgcggactg gccagctgcc tggcttgcac gtcgatcact tcacccgagt
     1261 tgatcttgaa cacggacacg gcctcggcca ggtgcctggc ctgctcttcc agcgacccgg
     1321 ccgccgcggc ggcctgttcg accagcgccg cgttctgctg cgtgacctcg tccatctgcg
     1381 acaccgcgcg gttgacctga tcgatgccgc cggactgctc ttccgaggcc gccgagatct
     1441 cgcccatgat gtcggtcacc cgcttcaccg aagtcacgat ctcctgcatg gtgttgccgg
     1501 cccgctccac ctgttcggaa cccacattga ccttgcccac cgagtcttcg atgaggacct
     1561 tgatttcctt ggcggcctgg gcgctgcgct gcgccagcga acgcacctcg cccgccacca
     1621 ccgcgaagcc cttgccctgc tcgccggcgc gcgccgcttc caccgccgcg ttcagcgcca
     1681 ggatattggt ctggaaggc
//
LOCUS       DASWPO010000174         6797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1200397, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000174 DASWPO010000000
VERSION     DASWPO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200397"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VGC69_09405"
     CDS             complement(<1..721)
                     /locus_tag="VGC69_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEY0295532.1"
                     /translation="MVASIGDQAVGELRDQGGLWSFQYTPAWLNSPHRYALSPHLPLR
                     AEPLLDGASVRPVQWYFDNLLPEEGRRTLLASNAKLDAADAFGLLTDYGAESAGSLTL
                     MPPDEQEAPAGGLRPLPDAALSLRIQQLPRISLAQGAAKRMSLAGAQHKLAVILQDDV
                     LSEPQGSRPSTHILKPEHAGPDYPHSVVNEWFIMRLADRVGLDVPGVQRRYVPEPVYL
                     IERFDRVSREGQVRRLHAIDAC"
     gene            complement(733..1008)
                     /locus_tag="VGC69_09410"
     CDS             complement(733..1008)
                     /locus_tag="VGC69_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY0295533.1"
                     /translation="MKRELTQARDLGPIVRAVRKVQGIRQDDAAGSIGVSENFLGKIE
                     RGGEAVQWGKLFHVLHGLGIKVILDIPDEAAQRLPPAASADEGSPWP"
     gene            complement(1146..2138)
                     /locus_tag="VGC69_09415"
     CDS             complement(1146..2138)
                     /locus_tag="VGC69_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0295534.1"
                     /translation="MTVETLRIEGISAPVSRVGLGTWAMGGWMWGGTDEAAAIATLRG
                     AVEQGINLIDTAPVYGFGRSEEIVGRALEGGLRDQAVIATKAALEWSDAGVRRNSTAA
                     RIRQEVEDSLRSLRTDRIDLYQVHWPDTLVPFEETAAELEKLRQAGKILAIGVSNYSP
                     QQMDAFSQAAPLVAVQPPYNLFERAIEADVLPYAKQHGLTVLAYGALCRGLLSGRMNA
                     ATQFEGDDLRKVDPKFQQPRFGQYLSAVQALAELARERHGKSVLALAIRWVLDQGPTV
                     ALWGARRPDQLEGIDDVFGWKLTAQDLADIDVLLATHVTDPVGPEFMAPPLRQA"
     gene            2269..3171
                     /locus_tag="VGC69_09420"
     CDS             2269..3171
                     /locus_tag="VGC69_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295535.1"
                     /translation="MPALDRAELADLNVFLTICRRRSFRQAAAELGVTPSALSHAMRN
                     LEARLGVRLLNRTSRSVAPSAAGTALAEQLEHGFAQITQALDAMEHFRATPKGRLRLN
                     VPRDASRLLVNPVLPDFFAAYPELALEITVEDRMVDIVAEGYDAGIRYGNTVPRDMIA
                     MPLTGPLRWVVVGSPAYLARRGRPRSPDDLLQHDCVRIRLGDNSLYKWELGSGGQAVE
                     IDVPGSLTANETDLAVDAALNGVGLAYCLQARVRQELADARLEVVLPEWAVSGPPLSM
                     YYASRRQTHPGLRQLIDMLRAQAQ"
     gene            complement(3165..4103)
                     /locus_tag="VGC69_09425"
     CDS             complement(3165..4103)
                     /locus_tag="VGC69_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY0295536.1"
                     /translation="MRLIVKSGGPGAHVEWKDLFAQVAPGIEVLDWYNPPADPGGIEY
                     AMVWDPDPGRLAALPDLKLIISSGAGIDHILADPQLPAHLPIARMVLEVTSIEMSEFV
                     LMSALMLVRDMKRATVSQARRHWDTFPAPRRSTTTRVGIMGLGSLGAHAAGMLMRVGF
                     PVSGWSRRPADLPGVTCYAGPEQLDAFLAGTDILVCLLPATEATRGILDADTFARLPR
                     GASVINAARGAHVVVPDLLAALDSGQLNQAVLDVFEPEPLAADSPLWTHPGVIVTPHI
                     ASTPGRRDRAMHAADLIRKQRQGEPLPNLYDRAAGY"
     gene            complement(4133..5188)
                     /locus_tag="VGC69_09430"
     CDS             complement(4133..5188)
                     /locus_tag="VGC69_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295537.1"
                     /translation="MKISRKCGAALLAGATLSGMLSAPAWAQQKTLYVGMNGGDMERA
                     FTQHVFPPFEQENNVKIVVVPGTSTEVLAKAQAYKNSPQMHVMFLDDGVMVRAVSMGL
                     CQKMTDGPELKQIYPAAILKDGMAAGIDAGMTGLAYNTKLFADKGWAPPTSWMDLADP
                     KYKGEVVFQSAATSTFGLHAFMMFNRIQGGSEKNTEPGFQKWAGTIGPNVVEYIPNTA
                     KLAEMIQSGEAGIFPLTPTAVARFKQRGLPVEYVQPKEGSVVLMVGECVIANNTEPDL
                     SQKLAHYLLSPKAQALMLEFSGQSPANRETPPPAGQEAFLGQLRGYMQTANQVDWDAI
                     NATRTDFNARWNRTVER"
     gene            complement(5257..6354)
                     /locus_tag="VGC69_09435"
     CDS             complement(5257..6354)
                     /locus_tag="VGC69_09435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0295538.1"
                     /translation="MNAAIETDVAVIGGGIVGSSAALFLRRRGLAATLIEAALCGAKA
                     SGVNYGGVRRQGRAPAQMPLAQRAHAIWGQLADFIGTDGEYVRSGHLKLARSQADLEA
                     LEQYRQSTRGYGMNLQMLDRAAVHRRFPWLGDSVAGGSMCPEDGHANPRLVSPAFARA
                     ARGLGAEVREQTRVASVEFDGTGFDVLTDTGPRIRARYVLNCAGAWAWKFAEHFGEPV
                     PETSIHPLMMVTEPLPVFMAPSLGMQGGGIYARQVARGNCIIGGGRGVPDQGDYARPE
                     RGAVAALLANTADLIPALRQAQVIRFWSGVEGSMPDHNPVLGPSARVPGLFHAFGFSG
                     AGFQIGPAAGEVLADLVAEGGTSIPIDAFCI"
     gene            complement(6351..>6797)
                     /locus_tag="VGC69_09440"
     CDS             complement(6351..>6797)
                     /locus_tag="VGC69_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0295539.1"
                     /translation="IAGADAAELASERAARAVLQDLGMAWSEARAAELERRLAGIAAT
                     RRALERAFPFPRDWAAGLDDDTQVCRCEEITVGTLRSTAAQGVDELNRLKALTRIGMG
                     RCQGRMCGVAAAELLAQAWGVEPAGVGRLRAQPPVKPIPIALEQAS"
BASE COUNT         1058 a         2321 c         2333 g         1085 t
ORIGIN      
        1 ggcaggcgtc gatcgcgtgc aagcgccgca cctggccctc acgcgagacg cgatcgaacc
       61 gttcgatgag atagactggc tcgggcacat agcggcgctg cacgccaggc acatccaggc
      121 cgaccctgtc ggccagccgc atgatgaacc attcgttgac gacagagtgc gggtagtctg
      181 ggcctgcgtg ctcaggcttg aggatatgcg tggacggccg gctcccttgc ggctcggaca
      241 agacatcgtc ctgcaaaatg acggccagct tgtgctgggc gcccgccagc gacatgcgct
      301 tggccgcgcc ttgcgcgagc gatatgcgcg gcaactgctg gatgcgcagg gaaagtgcgg
      361 cgtcgggcag cggacgcagg ccgcccgcgg gagcctcctg ctcgtccggc ggcatcagcg
      421 tcaacgagcc ggccgattcc gcgccgtagt cggtcaacag gccgaatgcg tccgcggcgt
      481 cgagcttggc gttggacgcc agcagggtac gccgcccttc ctcgggcagc aggttgtcga
      541 aataccattg caccggccgg acgctggcgc cgtcgagcaa gggctcggca cgcagaggca
      601 ggtgcgggct gagcgcgtag cgatgcgggc tgttcaacca ggccggcgtg tactggaagg
      661 accacaggcc accctggtcg cgcagctcgc ccacagcctg gtcgccgatg ctggcgacca
      721 atgccctccg ggttacggcc acggcgagcc ttcgtcagcc gatgccgcgg gaggcagccg
      781 ctgcgccgct tcgtccggga tgtccaggat gaccttgatc cccaggccgt gcagtacgtg
      841 gaagagcttg ccccactgca cggcttctcc accgcgctcg atcttgccga gaaaattctc
      901 gctgacgccg atgctgcccg ccgcgtcgtc ctggcggatg ccttgcacct tgcgcactgc
      961 ccgcacgatg ggcccaagat ccctggcttg tgtcagttcc cgcttcataa aatcctcacg
     1021 atcgtgagaa tttcatcata agcaggcctg gaaaacaata aatcctcata atcatgagga
     1081 ttttgttgtt ttaagtaatt gttccgtaaa aatcctcacg ataatgagga tttttacggt
     1141 agcgcttatg cctggcgcaa cggcggcgcc atgaactccg gcccgacggg gtcggtgaca
     1201 tgcgtggcca gcaggacatc gatgtcggcc aggtcctggg cggtgagctt ccagccgaac
     1261 acgtcgtcga tgccttcgag ctggtcggga cggcgcgcgc cccacaaggc caccgtggga
     1321 ccctggtcca gcacccagcg gatggccagc gccagcaccg acttgccgtg gcgctcgcgc
     1381 gccagctcgg ccagggcctg cacggcggac aggtactggc cgaagcgcgg ctgctggaac
     1441 ttgggatcga ccttgcgcag gtcgtcgccc tcgaactgcg tggcggcgtt catgcggccc
     1501 gacagcaggc cgcggcacaa ggcgccgtag gccagcacgg tcaggccgtg ctgcttggca
     1561 tagggcagca cgtcggcctc gatggcgcgc tcgaacaggt tgtagggcgg ctgcacggcg
     1621 accagcggcg cggcttgcga gaaagcgtcc atctgctgcg gcgaataatt gctgacgccg
     1681 atggccagga tcttgccggc ctggcgcagc ttctccagct cggcggcggt ttcctcgaag
     1741 ggcaccagcg tgtcgggcca gtgcacctgg tagaggtcga tgcggtcggt gcgcaggctg
     1801 cgcagcgagt cctcgacttc ctggcggatg cgggcggcgg tggaattgcg gcgcacgccg
     1861 gcgtcgctcc actccagggc ggctttggtg gcgatcaccg cctggtcgcg caggccgcct
     1921 tccagcgcgc ggccgacgat ctcctcggaa cggccgaagc cgtacacggg cgccgtgtcg
     1981 atcaggttga tgccttgctc gaccgcgccg cgcagggtgg cgatggcggc ggcctcgtcg
     2041 gtgccgcccc acatccagcc gcccatggcc caggtgccca ggcccacgcg cgataccggc
     2101 gcggaaatgc cttcgatacg cagggtttcc acagtcatgg tttgctcctt actaattcgg
     2161 aatttaatga cggaatggtc aggtgaatta atctatctca tatcttgatg agataagctc
     2221 atcaatccat tgagatcccc gcatacatcc gcgaaacgag acgccgccat gcccgccctg
     2281 gaccgcgccg aactggccga cctcaatgtc tttctcacca tctgccgccg gcgcagtttc
     2341 cgccaggcgg cagccgagct gggcgtcacg ccctcggcgc tgagccatgc catgcgcaac
     2401 ctggaggcgc ggctgggggt caggctgctc aatcggacca gccgctcggt ggcgcccagc
     2461 gctgccggca cggccctggc cgaacaactg gagcacggct tcgcgcagat cacccaggcg
     2521 ctggacgcga tggagcactt tcgcgccacg cccaagggcc ggctgcggct gaacgtgccg
     2581 cgcgatgctt cccgcctgct ggtcaacccg gtgctgccgg acttctttgc cgcctacccc
     2641 gagctggcgc tggagatcac cgtcgaagac cgcatggtgg atattgtcgc cgaaggctac
     2701 gacgccggca tccgctatgg caataccgtg ccgcgcgaca tgatcgccat gccgctgacc
     2761 ggcccgctgc gctgggtcgt cgtgggttcg ccggcctatc tggcccggcg cggccgcccg
     2821 cgatcccccg acgacctgct gcagcacgac tgcgtgcgca tacgcctggg cgacaactcg
     2881 ctctacaaat gggaactggg cagcggcggc caggccgtcg agatcgacgt gccgggctcg
     2941 ctgaccgcca acgaaaccga cttggccgtc gacgccgcgc tcaacggcgt ggggctggcc
     3001 tattgcctgc aagcgcgcgt gcgccaggaa ctggccgacg cccgcctgga ggtggtgctg
     3061 ccggaatggg ccgtgagcgg cccgccgcta tcgatgtact acgccagccg ccgccagacc
     3121 cacccgggcc tgcggcagct gatcgacatg ctgcgcgccc aggctcagta gcccgccgcg
     3181 cggtcgtaga gattgggcag cggctcgccc tgcctttgct tgcggatgag atcggccgcg
     3241 tgcatggcgc ggtcgcggcg gcccggcgtg gaggcgatgt gcggcgtgac gatcacgccg
     3301 ggatgcgtcc agagcggcga gtcggcggcc aggggttcgg gttcgaacac gtccagcacg
     3361 gcctggttga gctggccgct gtcgagcgcc gccagcaggt cgggcacgac cacgtgcgcg
     3421 ccgcgcgccg cgttgatgac ggaggccccg cgcggcaggc gcgcgaaggt atcggcgtcg
     3481 agaatgccgc gcgtggcttc ggtggccggc agcaggcaga ccaggatgtc cgtgcccgcc
     3541 aggaaggcgt cgagctgttc gggaccggcg tagcaggtca cgccgggcag gtcggccggc
     3601 cggcgcgacc agcccgatac gggaaagccc acgcgcatga gcatgccggc ggcatgcgcg
     3661 cccaggctgc ccaggcccat gatgcccacg cgcgtcgtgg tcgagcggcg cggcgcggga
     3721 aaggtgtccc agtgcctgcg cgcctggctg acggtggcgc gcttcatgtc gcgcaccagc
     3781 atcagcgccg acatcagcac gaactcgctc atctcgatgg acgtgacctc gagcaccatg
     3841 cgcgcgatgg gcagatgcgc cggcagctgc gggtcggcca ggatgtggtc gatgcccgcg
     3901 ccggagctga tgatgagctt gagatcgggc agggccgcca gccggcccgg gtcgggatcc
     3961 cagaccatgg cgtactcgat gccgccgggg tcggccggcg ggttatacca atccaggact
     4021 tcgatcccgg gcgccacctg ggcgaacaag tccttccact cgacatgggc cccggggccc
     4081 cccgatttca caatcaggcg catgacactc cctgctgatt cacgattcac gattagcgtt
     4141 cgacggtacg gttccagcgc gcgttgaagt cggtgcgcgt ggcgttgatg gcgtcccagt
     4201 ctacttggtt agcggtctgc atgtagccgc gtagttgccc caggaaagcc tcctggccgg
     4261 ccggcggcgg ggtttcgcgg ttcgccggcg actggccgct gaactccagc atcaaggcct
     4321 gcgccttggg cgacagcagg taatgcgcca gcttctgcga caggtcgggc tcggtattgt
     4381 tggcgatcac gcattcgccc accatcagca ccaccgaccc ctccttgggc tgcacgtact
     4441 cgaccggcag gccgcgctgc ttgaagcggg ccaccgccgt gggcgtgagc ggaaaaatgc
     4501 ccgcctcgcc gctctggatc atctcggcca gcttggccgt gttggggatg tactcgacca
     4561 cattggggcc gatggtgccg gcccacttct ggaagcccgg ctcggtgttc ttctcggagc
     4621 cgccctggat gcggttgaac atcatgaagg catgcaggcc gaaggtgctg gtggccgccg
     4681 actggaacac cacctcgccc ttgtacttgg gatcggccag gtccatccac gaggtgggcg
     4741 gcgcccagcc cttgtccgcg aacagcttgg tgttgtaggc caggcccgtc atgccggcgt
     4801 cgatgccggc ggccatgccg tccttgagga tggcggccgg atagatctgc ttgagctcgg
     4861 gaccgtcggt cattttctgg cacaggccca tggacacggc gcgcaccatg acgccgtcgt
     4921 ccaggaacat gacgtgcatc tgcgggctgt tcttgtaggc ctgggccttg gccagcacct
     4981 ccgtggaggt gcccggcacc accacgatct tgacgttgtt ctcctgctcg aagggcggga
     5041 agacgtgctg cgtgaaggcg cgctccatgt cgccgccgtt catcccgaca tacagggtct
     5101 tctgctgcgc ccaggcaggc gccgacagca tgcccgacaa ggttgcgccg gccagcaggg
     5161 ccgcgccaca tttgcgactg atcttcatca tcactccagt gttgactcac gcagttcatg
     5221 acgagacacg gcggcatgag cggcgcccgc gtatcgctag atgcaaaacg catcgatggg
     5281 tatggatgtc ccgccctcgg cgaccaggtc cgccagcacc tcgcccgcgg cggggccgat
     5341 ctggaagccc gcgccggaaa aaccgaaggc gtggaacagg cccggcaccc gggcgctggg
     5401 cccgagcacg gggttgtggt ccggcatgct gccctcgacg ccggaccaga agcggatgac
     5461 ctgcgcctgg cgcagggccg gaatgaggtc ggccgtattg gccagcaacg ccgccaccgc
     5521 gccgcgctcg ggccgggcat agtcgccctg gtcgggcacg ccgcggccgc cgccgatgat
     5581 gcagttgccg cgcgcgacct ggcgcgcata aataccgccg ccctgcatgc ccaggctggg
     5641 cgccatgaaa acgggcaagg gctcggtcac catcatcagg ggatggatgg acgtctcggg
     5701 cacgggttcg ccgaagtgct cggcaaattt ccaggcccag gcgcccgcgc aattgagcac
     5761 gtagcgggcg cggatccgcg ggcccgtgtc ggtcagcacg tcgaagcccg tcccgtcgaa
     5821 ttccaccgac gccacgcgcg tctgctcgcg cacctcggcg cccagcccgc gcgcggcccg
     5881 ggcgaaggcg ggcgatacca ggcggggatt ggcgtggccg tcttcaggac acatcgaccc
     5941 gcccgccacg ctgtcgccca gccagggaaa gcggcggtgc acggcggcgc ggtccagcat
     6001 ctgcaggttc atgccgtagc cgcgcgtgga ctgccggtac tgttcgagcg cctccagatc
     6061 cgcctggctg cgcgccagct tgaggtggcc cgaacgcacg tattcgccat cggtgccgat
     6121 gaaatcggcc agctgccccc agatggcgtg cgcgcgctgc gccagcggca tctgcgcggg
     6181 cgcgcgcccc tgccggcgca cgccgccata gttgacgccg ctggccttgg cgccgcacaa
     6241 ggccgcctcg atcagggtcg cggccaggcc gcggcggcgc aggaacaggg ccgcgctgct
     6301 gccgacgatg ccgccgccga tgacggccac atccgtttcg atggcggcgt tcacgatgcc
     6361 tgctccaggg cgatggggat gggcttgacc ggcggctgcg cgcgcagccg tcccacgccg
     6421 gctggttcga cgccccaggc ctgcgccagc aattcggccg ccgcgacgcc gcacatgcgg
     6481 ccctggcagc gccccatgcc gatgcgcgtc agcgccttga gccggttgag ttcatcgacg
     6541 ccctgcgcgg cggtcgaacg caaggtgcct accgtgattt cctcgcagcg gcagacctgg
     6601 gtgtcatcgt ccaggccggc ggcccagtcc cggggaaagg ggaaggcgcg ctccagcgcg
     6661 cggcgcgtgg cggcgatgcc ggccagcctg cgctccagtt cggccgcgcg cgcttcggac
     6721 cacgccattc ccaggtcctg caagacggcc cgggccgccc gctcgctggc cagctcggcc
     6781 gcgtccgccc cggcgat
//
LOCUS       DASWPO010000175         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_753709, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000175 DASWPO010000000
VERSION     DASWPO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753709"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGC69_09445"
     CDS             complement(<1..305)
                     /locus_tag="VGC69_09445"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0295540.1"
                     /translation="MIDALAASLGLAPGDPAFWMPLVFMAILLLLIAAGIVFDGFDIG
                     VGLLLQLAPTESRPQMLAMLSAWRDANEFWPLLGVGLFCAAFPFAWGPVLGKLYGPLA
                     "
     gene            complement(302..1642)
                     /locus_tag="VGC69_09450"
     CDS             complement(302..1642)
                     /locus_tag="VGC69_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0295541.1"
                     /translation="MDITPLLLARIQFLASFSFLALFMALALALSWILLFFKLSARLT
                     GHAGWTAAYRFWVRVFALSFVLSLAACLPVLIQLGSIWSGLMDKIGNVAGPLLGYAVL
                     SVFVLKSCFLGVMLFGQRRVSDTIHTLAVFMVALGMLLAVAWLVAVHSWMQTPDGAAV
                     VDGRYQVYDWFEVIFNPSFGWSLLLTVLGAALAAAFLMMGVTALQARRRPSGDGERNA
                     FKTALVVAVLASLLQALAAVGIGDVVSRYQPVKAATLAGYWHSGDEPELILLGWPDAQ
                     TQTNRVLVSIHGMGGRWLGRDAQGYLGLDKFTGPRPPVALSFWLLRGSALLGMLMAAV
                     SVLTLMRTMWRRMDLAVLSAWWHKVLVGMTFSGCLSVVLGWGVSLVGLLPFVVNRSIN
                     LIDVLGPVSTGTLVYGLVGYAALYAVLIVAFLGMLLHAVRYGVVPVRKTTGGAA"
     gene            1777..3579
                     /locus_tag="VGC69_09455"
     CDS             1777..3579
                     /locus_tag="VGC69_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY0295542.1"
                     /translation="MTTLGTPAPTEAPAAAAPPANHAASNFLRPIIEADLAADRYQGK
                     RWSGKPGPAAAQSQGAPDPARIRTRFPPEPNGYLHIGHAKSICVNFGLAREFGGVCHL
                     RFDDTNPEKEDQEYVDAIAEAVSWLGFDWKADGNDNKYFASDYFDWMYEFAEALIDAG
                     HAYVDGQSADAIRANRGTLTEPGANSPWRDRPAAESLALLREMRDGRHPDGSLVLRAR
                     IDMASPNINLRDPVIYRVRHAHHHRTGDKWCIYPMYTFAHPIEDALEGITHSICTLEF
                     EDQRPFYDWILARLADLGKLARPLPRQYEFARLNMTYVVTSKRKLLQLVKEGHVDGWD
                     DPRMPTIFGLRRRGYTSSAIRLFCDRTAVSKSDSRIDYSLLEQAVRDDLDPVAQRSVA
                     VLDPLKLVIANYPEGQSETCHAARNPHDPAAGNREFLFSRELWIERDDFREEAPKKYF
                     RLFPGNLVRLKYGYVVKCTGCTKDADGNVIEVQAEYLPETRSGTSGADSVKVKGTITW
                     VSAAHAVPATVHLYDRLFADPMPDAGDKDFLACLNPNSRKTVQAWLEPGTVAAPGATW
                     QFERLGYFTADRVDSTPETPVLNRIVTLRDSWGA"
     gene            3581..>3712
                     /gene="minC"
                     /locus_tag="VGC69_09460"
     CDS             3581..>3712
                     /gene="minC"
                     /locus_tag="VGC69_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936549.1"
                     /note="blocks the formation of polar Z-ring septums;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEY0295543.1"
                     /translation="MSDDLALDFKSATLYAVRLVLHSADAARLDEALAQRMKDAGGFF
                     "
BASE COUNT          702 a         1386 c         1146 g          478 t
ORIGIN      
        1 gccagcggcc cgtacagctt gcccagcacc ggcccccagg caaacggaaa cgccgcgcag
       61 aacaggccca cgcccagcag gggccagaat tcgttggcat cgcgccaggc gctcagcatg
      121 gccagcatct gcggacgcga ctccgtcggc gccagctgca gcaagaggcc cacgccgatg
      181 tcgaagccgt cgaacacgat gcccgctgcg atcagcagca gcaggatcgc catgaacacc
      241 aggggcatcc agaacgcggg atcgccgggc gccaggccca gcgaagcggc caatgcgtcg
      301 atcatgccgc tccccccgtg gtcttgcgca ccggcaccac gccgtagcgc accgcatgca
      361 gcagcatgcc cagaaacgcg acgatcagca ccgcgtacaa ggccgcgtag cccaccagcc
      421 cgtagacgag cgtgcccgtg ctcaccggac ccagcacgtc gatcagattg atgctgcggt
      481 tgaccacgaa gggcagcagc cccaccagcg acacgcccca gcccagcacc accgacaggc
      541 agcccgagaa cgtcatgccc accagcacct tgtgccacca ggcgctcagc accgccaggt
      601 ccatgcgccg ccacatggtg cgcatcaggg tcagcaccga cacggccgcc atcagcatgc
      661 ccagcagcgc cgagccgcgc aacagccaga acgacagggc caccggcggg cgcgggccgg
      721 tgaatttatc cagccccagg tagccctgcg cgtcgcggcc cagccagcgg ccgcccatcc
      781 cgtggatgga caccaggacc cggttggtct gcgtctgcgc gtcgggccag cccagcagga
      841 tcagttcggg ctcgtcgccg ctgtgccagt agccggccag cgtggcggcc ttgaccggct
      901 ggtagcggct gaccacgtcg ccgatgccca cggccgccag ggcctgcagc agcgaggcca
      961 gcaccgccac gaccagcgcc gtcttgaagg cattgcgctc gccgtcgccg gacgggcggc
     1021 gccgggcctg cagggccgtc acgcccatca tcaggaaggc cgcggccagc gccgcgccca
     1081 gcaccgtcag caacaggctc cagccgaaag agggattgaa gatcacctcg aaccagtcat
     1141 agacctggta gcggccgtcc accacggcgg cgccgtcggg cgtctgcatc caggagtgca
     1201 ccgcgaccag ccaggccacg gccaggagca tccccagggc caccatgaag acggccaggg
     1261 tgtggatggt gtcggacacg cggcgctggc cgaacagcat cacgcccagg aagcacgact
     1321 tcagcacgaa caccgatagc acggcatagc ccaggagcgg tccggcgacg ttgccgatct
     1381 tgtccatgag cccgctccag atgctgccga gctggatcag caccggcagg caggccgcca
     1441 ggctcagcac gaaggacagg gcgaagacgc gcacccagaa acggtaggcc gccgtccaac
     1501 cggcatggcc ggtcagccgg gcgctgagct tgaaaaacaa cagtatccag gacagcgcga
     1561 gggccagggc catgaacaac gccaggaaac tgaagctggc caggaactgg atccgggcca
     1621 gcaggagcgg ggtgatatcc atgatgggcg ctagtgtaga gcctgttatg tcgccaagaa
     1681 ggtcaatctc tgtggtttcc tggggggttc tcggcggttt tcggggcgtg gacgtggcaa
     1741 aattgacgct ttgttgctgt ttttcactat atacgcatga ccaccctcgg cactcctgcg
     1801 cctaccgaag ctccggctgc ggccgctccc ccagcaaacc acgcggccag caatttcctg
     1861 cgccccatca tcgaggccga cctggcggcc gaccgctacc agggcaagcg ctggtccggc
     1921 aagcccggcc cggccgccgc gcagagccag ggcgcgcccg acccggccag gatccgcacc
     1981 cgcttcccgc ccgagcccaa cggctacctg cacatcggcc acgccaagag catctgcgtg
     2041 aacttcggcc tggcgcgcga attcggcggg gtctgccacc tgcgcttcga cgacaccaac
     2101 cccgagaagg aagaccagga atacgtcgac gccatcgccg aggccgtgag ctggctgggc
     2161 ttcgactgga aggccgacgg caacgacaac aagtacttcg ccagcgacta cttcgactgg
     2221 atgtacgagt tcgccgaggc gctgatcgac gccggccacg cctatgtgga cggccagagc
     2281 gccgacgcga tccgcgccaa ccgcggcacc ctcaccgagc cgggcgcgaa ctcgccctgg
     2341 cgcgaccgcc cggccgccga gtcgctggcc ctgctgcgcg aaatgcgcga cggcaggcac
     2401 cccgacggca gcctggtgct gcgcgccagg atcgacatgg cctcgcccaa catcaatctg
     2461 cgcgaccccg tcatctaccg cgtgcgccac gcccaccacc accgcacggg cgacaagtgg
     2521 tgcatctacc ccatgtacac cttcgcgcat cccatcgagg acgcgctcga aggcatcacg
     2581 cacagcatct gcacgctgga gttcgaagac cagcgcccct tctacgactg gatcctggcc
     2641 cgcctggccg atctcggcaa gctggcccgg cccctgcccc gccagtacga gttcgcccgg
     2701 ctgaacatga cctatgtggt caccagcaag cgcaagctgc tgcagctggt caaggaaggc
     2761 cacgtcgacg gctgggacga cccccgcatg cccaccattt tcggcctgcg ccggcgcggc
     2821 tacacatcct cggccatccg cctgttctgc gaccgcacgg ccgtgtccaa gtccgactcg
     2881 cgcatcgact acagcctgct cgagcaggcc gtgcgcgacg acctcgaccc cgtcgcgcag
     2941 cgctcggtgg cggtgctcga cccgctcaag ctggtcatcg ccaactaccc cgagggccag
     3001 tcggaaacct gccatgccgc gcgcaacccg catgacccgg ccgccggcaa ccgcgaattc
     3061 ctcttctcgc gcgagctctg gatcgaacgc gacgacttcc gcgaagaagc gcccaagaag
     3121 tacttccgcc tcttccccgg caacctggtg cgcctgaagt acggctacgt cgtcaagtgc
     3181 accggctgca ccaaggacgc cgacggcaat gtcatcgagg tgcaggccga gtacctgccc
     3241 gaaacccgca gcggcacgtc cggcgccgac agcgtcaagg tcaagggcac catcacctgg
     3301 gtcagcgccg cgcatgccgt gccggccacc gtgcacctgt acgaccgcct cttcgccgac
     3361 ccgatgcccg atgccggcga caaggacttc ctcgcctgcc tcaaccccaa ctccaggaag
     3421 accgtgcagg cctggctgga acccggcacc gtggccgcgc ccggcgccac ctggcagttc
     3481 gagcgcctgg gctatttcac cgccgaccgc gtcgactcca cgcccgaaac gccggtgctc
     3541 aaccgcatcg tgaccttgcg cgactcgtgg ggggcttgaa gtgagcgacg atctggccct
     3601 ggacttcaag agcgcgaccc tctacgccgt gcgcctggtg ctgcacagcg ccgacgccgc
     3661 ccgcctggac gaagcgctgg cgcaacgcat gaaggacgcc ggcggcttct tc
//
LOCUS       DASWPO010000176         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2183903, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000176 DASWPO010000000
VERSION     DASWPO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183903"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..731
                     /gene="tsaE"
                     /locus_tag="VGC69_09465"
     CDS             201..731
                     /gene="tsaE"
                     /locus_tag="VGC69_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY0295544.1"
                     /translation="MSEPLALLSLHLPDEAATDGLAGRLAPLVSGAAGLPAGGRIHLQ
                     GDLGAGKTAFTRALLRACGIQGRIKSPSYALLESYEVSNLYFYHFDFYRFSDSREWLD
                     AGFRDLLRDDAVVLIEWPERAGSLLAPPDLLITLSYAQRGRDATLAAHTARGQTWLNA
                     LALPKSSPPAPPPAGA"
     gene            668..2089
                     /locus_tag="VGC69_09470"
     CDS             668..2089
                     /locus_tag="VGC69_09470"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0295545.1"
                     /translation="MVERARPAEVLPASAATRRRLISVATTLLVLPVLPRLAQAATIL
                     AVRTWPADEYTRVTLELDSELKAEHFTLENPNRLVVDIQGLTASSTLNDLVSKVRPDD
                     PYIRGLRVAQNRPNVVRLVFDLKQAVAPQVFTLKPVADYQYRLVLDLYPKVAQDPLMA
                     MVNRPVAPDVDDPLARVLDQIANNPAARSADPAPMPMAKGQEAPPVPPGAPLNVIVHG
                     AGKQDAPPAPPTRQANRKRMLTIALDPGHGGEDPGAIGATGLREKDVVLRIARRLKAM
                     IDNQPAMRAYLTRDDDYFVPLNVRVEKARRVRADLFISIHADAFIKPTASGSSVFALS
                     EHGASSTQARWMANRENSADLIGGVNLGSHDAQVAKVLLDLSTTAQINDSLKVGTAFL
                     DELGKINRLHSNEVEQAGFAVLKAPDIPSVLVETAFISNPREEALLRTDSHQEKVAVA
                     LMSGIDRYFTANPPLARTLADAS"
     gene            2258..3046
                     /locus_tag="VGC69_09475"
     CDS             2258..3046
                     /locus_tag="VGC69_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0295546.1"
                     /translation="MALHALKPSPSVPSDAERRVREDLAAAYRLVAHFGLDDSIYTHI
                     SARVPGTLDQFLINPFGMLFKDITASSLVKIDTEGRILEPTDYSVNPAGFTIHSAVHM
                     ARHDAECVLHTHTVAGVAVSSLQCGLQPCNQWALQFYNRVVYHEFEGIALNLDERERL
                     VADLGPDAKAMILRNHGLLTCGDSVAEAFILMMNLERACRVQMSIQASGQPIHPVPVE
                     VCELTANQYTGWVKHCKTIGKDPHEREWKAYLQRLEPTVSSYRD"
     gene            3147..>3848
                     /locus_tag="VGC69_09480"
     CDS             3147..>3848
                     /locus_tag="VGC69_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295547.1"
                     /translation="MKRRQFIQMGTAGLGLSIAGVPLHVLAQGRAGGTLNVIVQPEPP
                     GLMLGIIQNGPTQMISGNIYEGLLRYDEKLAPQPLLATEWSVSPDKLTYTFKLKPNVK
                     WHDGQPFTADDVVFSADVFLRKTHARLRNNLAAVDTIKALDPMTVEFKLKYPFDSFMG
                     IFETGSMPMVPKHVYENTDFLSNPANNTPIGNGPYKFKEWVKGSYIQLTANTDYHVQG
                     VPIIADIYFQVIPYAA"
BASE COUNT          711 a         1449 c         1086 g          602 t
ORIGIN      
        1 gaaggtgtct ttttcgatgc gggtcttcat ggcgatgcaa ctccgtgtcg gcgccccgcc
       61 ggcaacaagg ccgtccaggg catggccgga acgattccgg acatttacaa ataagaatcg
      121 atatcaatac aagcgtagca gaagccgcgc cgttcctata gcatcgcggc acgcccagtc
      181 gttttaccac ccctgccgac atgtccgaac cgctcgccct gctttccctg cacctgcccg
      241 acgaggcggc caccgacggc ctggccggcc ggctcgcgcc cctggtcagc ggcgcggcgg
      301 gcctcccggc cgggggacgc atccacctcc agggcgacct gggcgcgggg aagaccgcct
      361 tcacccgggc actgttgcgc gcatgcggca tccagggccg gatcaaaagt cccagctacg
      421 cgctgcttga atcttatgaa gtttctaact tatacttcta tcactttgat ttttatagat
      481 ttagcgattc gcgcgaatgg ttggatgccg ggtttcgcga tttgctgcgc gacgatgcag
      541 tggtattgat cgaatggccc gagcgtgccg gcagcttgct ggccccgccc gacctgctga
      601 tcacactctc ctacgcgcag cgtggtcgtg acgcaaccct cgccgcccac accgcccgag
      661 gtcaaacatg gttgaacgcg ctcgccctgc cgaagtcctc cccgccagcg ccgccacccg
      721 ccggcgcctg atcagcgtcg ccacgacgct gctcgtgctg cccgtgctgc cgcgcctggc
      781 gcaggcggcc accatcctgg cggtgcgcac ctggcccgcc gacgaataca cccgcgtcac
      841 gctcgagctc gacagcgagc tcaaggccga gcacttcacg ctcgaaaacc cgaatcgcct
      901 ggtcgtcgac atccagggcc tgacggccag cagcacgctc aacgacctgg tttccaaggt
      961 ccgtcccgac gacccctaca tccgcggcct gcgcgtggcg cagaaccgcc ccaacgtcgt
     1021 gcgcctggtg ttcgacctca agcaggccgt ggcgccgcag gtcttcacgc tcaagcccgt
     1081 ggccgactac cagtaccgcc tggtgctcga cctgtacccc aaggtggcgc aggacccgct
     1141 gatggcgatg gtcaacaggc ccgtcgcccc cgacgtcgac gacccgctgg cgcgcgtgct
     1201 cgaccagatc gccaacaacc ccgcggcccg ttccgccgac cctgcgccga tgccgatggc
     1261 caaggggcag gaagccccgc ccgtgccgcc tggcgcgcca ttgaacgtga tcgtgcatgg
     1321 cgccgggaag caggacgccc cgcccgcgcc ccccacgcgc caggccaacc gcaagcgcat
     1381 gctgaccatc gcgctcgatc ccggccacgg cggcgaagac ccgggcgcca tcggcgccac
     1441 cggcctgcgt gaaaaagacg tggtgctgcg catcgcgcgc cgcctcaagg cgatgatcga
     1501 caaccagccc gccatgcgcg cctacctcac gcgcgacgac gactacttcg tgcccctgaa
     1561 cgtgcgcgtg gaaaaggccc gccgcgtgcg cgccgacctg ttcatctcca tccacgcgga
     1621 cgccttcatc aagcccaccg ccagcggctc gtcggtgttc gccctgtccg aacacggcgc
     1681 ctccagcacg caggcccgct ggatggccaa tcgcgagaac agcgccgacc tcatcggcgg
     1741 cgtcaacctc ggctcgcacg acgcccaggt ggccaaggtg ctgctcgacc tctccaccac
     1801 cgcgcagatc aacgactcgc tcaaggtcgg caccgccttc ctcgacgaac tgggcaagat
     1861 caaccgcctg cacagcaacg aagtcgaaca ggccggcttc gccgtgctca aggcccccga
     1921 cattccctcc gtgctggtcg agaccgcctt catcagcaac ccgcgcgaag aggccctgct
     1981 gcgcaccgac agccaccagg aaaaagtcgc cgtggcgctg atgagcggca tcgaccgcta
     2041 cttcaccgcg aacccgccgc tggcgcgtac gctggccgac gcctcctgat ccgatctccg
     2101 gacaaacccc gaagttgcgg gcataccctg atttacacct ttccggggtg gaaatagcct
     2161 agtcacacct gtcgacagtt gacagagata cgctccgccg accgcgaagg cgtaccaggc
     2221 aacgcaattt ttccacgatc cagaaaggga tgcctccatg gcgctgcatg cactcaagcc
     2281 gtctccctcc gtcccgtccg acgcggaacg ccgcgtgcgc gaggatctcg ccgccgcata
     2341 ccgcctggtg gcccacttcg ggctcgacga cagcatctac acccacatct cggcgcgcgt
     2401 gcccggcacg ctggaccagt tcctgatcaa cccgttcggc atgctgttca aggacatcac
     2461 cgcctcgtcg ctggtgaaga tcgacaccga gggccgcatc ctcgaaccga ccgactactc
     2521 ggtcaatccc gccggcttca ccatccacag cgccgtgcac atggcgcggc acgacgccga
     2581 atgcgtgctg cacacccaca ccgtggccgg cgtcgccgtg tcctcgctgc aatgcggcct
     2641 gcaaccctgc aaccagtggg cgctgcagtt ctacaaccgg gtcgtctacc acgagttcga
     2701 gggcatcgcg ctcaacctcg atgaacgcga gcgcctggtc gccgacctcg gccctgacgc
     2761 caaggccatg atcctgcgca accacggcct gctgacctgc ggcgactcgg tggccgaggc
     2821 cttcatcctg atgatgaacc tcgaacgcgc ctgccgcgtg cagatgtcca tccaggccag
     2881 cggccagccc atccatcccg tgcccgtcga ggtctgcgaa ctgaccgcca accaatacac
     2941 gggctgggta aagcactgca agaccatcgg caaggacccg cacgaacgcg agtggaaagc
     3001 ctacctgcag cgcctggaac ccaccgtttc gtcctaccgg gactgagtct cctccctcgc
     3061 cgcctccccg atcacggccc gtgctccgca cggccgcgtc ggccttcctg ttgtcgtcat
     3121 tcgccctgaa caaggagcat caagcaatga aacgtcgcca attcatccag atgggaacgg
     3181 ccggcctcgg cctgagcatc gccggagtgc ccctgcatgt gctggcccag ggccgcgccg
     3241 gcggcacgct gaacgtcatc gtgcaacccg agccgcccgg cctgatgctg ggcatcatcc
     3301 agaacggccc cacccagatg atctccggca acatctacga gggcctgctg cgctacgacg
     3361 agaagctcgc cccccagccc ctgctggcca ccgagtggag cgtcagcccc gacaagctga
     3421 cctacacctt caagctcaag cccaacgtca agtggcacga tggccagccc ttcacggccg
     3481 acgacgtggt gttctcggcc gacgtgttcc tgcgcaagac gcatgcgcgg ctgcgcaaca
     3541 acctggccgc cgtcgacacc atcaaggcgc tggacccgat gacggtggaa ttcaagctga
     3601 agtacccctt cgactcgttc atgggcatct tcgagaccgg ctccatgccc atggtgccca
     3661 agcacgtcta cgaaaacacg gacttcctct ccaacccggc caacaacacc ccgatcggca
     3721 acggcccgta caagttcaag gagtgggtca agggctcgta catccagctc acggccaaca
     3781 ccgactatca cgtgcagggc gtgcccatca tcgccgacat ctacttccag gtgatcccct
     3841 acgcggcc
//
LOCUS       DASWPO010000177         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_398949, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000177 DASWPO010000000
VERSION     DASWPO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398949"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VGC69_09485"
     CDS             <1..767
                     /locus_tag="VGC69_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY0295548.1"
                     /translation="QVQSLSRTTAIIRALASARSARMRLTDIARATGLTLSTTTRLLA
                     AMAAERLVEAHDGGKTWKLGSELVYMGLSAARQQPLIRQASPHIEALAQETGDTAYLT
                     VRCGDDSVCAFRAAGTHRAQAYATYIGSRRPLGIGAGGLALLVALPAEEIDAILQRNA
                     SKLAGYPHAGLQSLRHDMTAAVTRGYTYTENHLSGGVHAMGLAVRSTQGSLVAAVSVS
                     AIAARLLEPRRGRIAQLLRDTAARIAIDLDATSLEE"
     gene            complement(786..1712)
                     /locus_tag="VGC69_09490"
     CDS             complement(786..1712)
                     /locus_tag="VGC69_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295549.1"
                     /translation="MDLTSLTLLVEIIDSGNLSQAARKLKMTRANVSYHLAQLEKAAG
                     VQLVHRTTRRVEPTEVGLSLYKHGLAIRNEMRAAQEAIANLSQGLQGRVGLSVPSGYG
                     QLVMSDWLIEFKRLYPGIVLDVLFENRADNLRDEVDIAIRVIPEPPPSLVARSLGDVR
                     YLACASREFARRHGLPRTLPALRDTPLITSGVVGRQLRLSAYRGDERQEVMLEPTLIS
                     EHFPFLRQGILAGLGVGLVPDYVVRDQVACGEVVTTLDEYRLSIFGTHMYLLYMPNRH
                     QTRAVRTCIDFLLEKAGQARDQTHKENQTKTL"
     gene            1863..>2331
                     /locus_tag="VGC69_09495"
     CDS             1863..>2331
                     /locus_tag="VGC69_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0295550.1"
                     /translation="MSASSPDSVVATRLEGDVLLVTIDNPPVNALGVAVRRGLLDAMA
                     QADADAAVKAVLIVGMGKAFIAGADIREFGKPPLPPSLPEVCNRIEACAKPVVAAIQG
                     PALGGGLEVAMSAHYRLALPAAKLGLPEVTLGLLPGAGGTQRAPRLMGVQAAAE"
BASE COUNT          369 a          751 c          802 g          409 t
ORIGIN      
        1 gccaggtgca aagcctgagt cggaccactg ccatcatccg tgcactggcc tcggcgcgta
       61 gtgcccgcat gcgtctgact gacattgcgc gagccaccgg gctgacgctg tcgaccacca
      121 cgcggttgct ggcggccatg gcggccgagc gcttggttga ggcgcacgac ggcggcaaga
      181 cctggaaact cggctcagag ctcgtctata tgggtttatc ggccgcgcgg caacagccgc
      241 tgatccgcca ggcgtcgccg cacatcgagg cgctggcgca agaaacgggc gatacagcct
      301 atctgaccgt gcgttgcggc gatgattcgg tctgcgcctt ccgagccgcc ggcacgcacc
      361 gcgcacaggc ctacgccacc tatatcggta gccgtcggcc gctggggatc ggcgcgggcg
      421 gtctggcgtt gctggtcgct ttgcctgccg aggagatcga tgctattttg cagcgcaacg
      481 ccagcaagct ggcgggttat ccgcacgcgg gcctgcagtc gttgcgtcat gacatgaccg
      541 ccgcggtcac gcgtggctat acctacacgg aaaatcatct ttccggcggg gtgcatgcaa
      601 tggggcttgc agtgcgctcg acgcagggat cgctggtcgc cgctgtcagc gtgtcggcga
      661 tcgcggcacg gctgctggag ccgcgccgcg gtcgcatcgc gcaactgctg cgggacacgg
      721 cggcgcgcat tgccatcgac ttggatgcga caagcctgga ggaataagat aaaccggcta
      781 agcagtcaga gggtcttggt ctggttttcc ttgtgggttt gatcgcgcgc ttggccggcc
      841 ttctccagca gaaagtcgat gcaggtgcgc acggcccggg tctggtgccg gttgggcatg
      901 tacagcaggt acatatgcgt gccgaagatg ctgaggcggt actcgtccag cgtggtcacg
      961 acctcgccgc aggcgacctg gtcgcgcacc acgtagtcgg gcaccaggcc cacgcccagt
     1021 ccggccagga tgccctggcg caggaagggg aagtgttccg agatcagcgt gggctcgagc
     1081 atgacctcct ggcgttcgtc gccccggtag gcggagaggc gcagttgccg ccccacgacg
     1141 ccggaggtga tcaggggcgt gtcgcgcagc gccggcaggg tgcgcggcag gccatgccgc
     1201 cgcgcgaatt cccgcgaggc gcaggccagg tagcgcacgt cgcccaggct gcgcgccacc
     1261 agcgatggcg gcggctcggg aatcacgcgg atggcgatgt ccacttcgtc gcgcagattg
     1321 tcggcgcggt tctcgaagag cacgtcgagc acgatgccgg ggtagaggcg cttgaactcg
     1381 atcagccagt cgctcatcac cagctggccg tagccgctgg gcacgctcag gcccacgcgg
     1441 ccctgcaggc cctggctcag gttggcaatg gcttcctggg cggcgcgcat ttcgttgcgg
     1501 atggccaggc cgtgcttgta caggctcagc cccacttcgg tgggctcgac gcggcgcgtg
     1561 gtccggtgca cgagctgcac cccggcggcc ttttccagct gggcgaggtg ataactgaca
     1621 tttgcccgcg tcatcttgag cttgcgcgcg gcctggctca ggttgccgct gtcgatgatt
     1681 tccaccagca gggtcaggga agtgagatcc atggacattt gtcaaaaaat attagacaga
     1741 ttgtcaacat ttgattggat tgtaaagttt atttgtcgca ggcaaaatct ttagtctgtt
     1801 ccccaaaact caaggctgcg cggttcgccg gcgcagccgg cacaacatcg gagacgcgca
     1861 gcatgtctgc ttcaagccct gattccgtgg tcgccacccg cctcgaaggc gatgtgctgc
     1921 tggtcaccat cgacaatccc cccgtcaacg cgctgggcgt ggccgtgcgc cgcggcctgc
     1981 tggacgccat ggcgcaggcc gacgccgatg cggccgtcaa ggccgtgctc atcgtgggca
     2041 tgggcaaggc cttcatcgcg ggcgccgaca tccgcgaatt cggcaagccg ccgctgccgc
     2101 cctcgctgcc cgaggtgtgc aaccgcatcg aggcatgcgc caagcccgtg gtcgcggcca
     2161 tccagggtcc ggccctgggt ggcggcctgg aagtggccat gtccgcacac taccgcctgg
     2221 ccttgccggc cgccaagctg ggtttgcccg aggtgaccct gggcctgctg cccggcgcgg
     2281 gcggcaccca gcgcgcgccg cgcctgatgg gcgtgcaggc ggccgccgaa c
//
LOCUS       DASWPO010000178         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2632142, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000178 DASWPO010000000
VERSION     DASWPO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632142"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1296)
                     /locus_tag="VGC69_09500"
     CDS             complement(40..1296)
                     /locus_tag="VGC69_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0295551.1"
                     /translation="MNKILNVAVGAVALLAATGAQAADTIKWLHVEVNPTQIAIWNEA
                     GHAFEATHPGVKVEPQYLENEAYKSKLTTLLQSRDKPAMFYSWAGGVLSAQVQAGVIE
                     DLTDKLRGYSDTLTPTALAAFTVNGRIYGVPQAITEVGFLYNKALFAKAGLDADKIKS
                     WDDFLAAVKTLKAAGITPLTAGGADKWPLSFFWSYLSLRQGGKAVFDAAMKDQNGGFA
                     GPDFVKAGEYFKQLVDLQPFQNGFLGAKHLPAIGLFADGKAAMTLAINVIYNQQAAIA
                     ADKKGLAPDQIGWLDFPTLPGGKGLATDTLGGVVGWVVSKDAPPATADFIKSFVSPEV
                     QAKLTAAGFIVPVVKGADQSLNSPFMRRIGEELAHSTYHQNFYDQVLGPSVGRTVNDV
                     VAELAGGTMTPDAAAKEVEAAWKQGN"
BASE COUNT          281 a          547 c          571 g          332 t
ORIGIN      
        1 cggccggcca cgacagagga atgcgatgcc atccggtgcc tagtttccct gtttccaggc
       61 ggcttccact tccttggcgg cggcgtccgg cgtcatcgtg ccgccggcca gctccgccac
      121 cacatcgttc accgtgcggc cgaccgatgg gccaagcacc tggtcgtaga aattctggtg
      181 gtaggtcgaa tgcgccagct cctcgccgat gcgccgcatg aacgggctgt tcagcgactg
      241 gtcggcgccc ttgaccacgg gcacgatgaa gccggcggcg gtcagcttgg cctgcacctc
      301 gggcgagacg aaggacttga tgaaatccgc ggtggccggc ggcgcgtcct tgctgaccac
      361 ccagccgacg acgccgccca gcgtgtccgt cgccagcccc ttgccgccgg gcagggtcgg
      421 gaagtccagc cagccgatct ggtcgggggc caggcccttc ttgtcggcgg cgatggcggc
      481 ctgctggttg tagatgacgt tgatggccag cgtcatcgcc gccttgccgt cggcgaacag
      541 gccgatggcg ggcaggtgct tggcgccgag gaagccgttc tggaacggct gcagatcgac
      601 gagctgcttg aaatactcgc cggccttgac gaagtcggga ccggcgaaac cgccgttctg
      661 gtccttcatc gcggcgtcga acacggcctt gccgccctgg cgcagcgaca ggtaggacca
      721 gaagaagctg agcggccatt tgtcggcgcc gcccgccgtc agcggcgtga tgccggcggc
      781 cttcagcgtc ttcaccgcgg ccaggaagtc atcccagctc ttgatcttgt cggcatccag
      841 gccggccttg gcgaacagcg ccttgttgta gaggaagccg acctcggtga tggcctgcgg
      901 cacgccgtag atgcggccgt tgacggtgaa ggcggccagc gccgtgggcg tcagcgtgtc
      961 gctgtagccg cgcagcttgt cggtgaggtc ctcgatcacc cctgcctgga cctgcgcgct
     1021 cagcacgccg ccggcccagc tgtagaacat cgccggcttg tcgcgggatt gcagcagcgt
     1081 ggtcagcttg gatttgtagg cctcgttctc gagatattgc ggctccacct tcacgccggg
     1141 atgggtggcc tcgaaggcat ggccggcctc gttccagatg gcgatctgcg tggggttcac
     1201 ctccacatgc agccacttga tggtatcggc ggcctgcgcc ccggtggccg cgagaagggc
     1261 cacggcaccg actgcgacgt tgagaatttt gttcacgtct tccccccttg cccgctccga
     1321 ggctggcccg gctctcaggc tgggcgaggg gacactgcca gcaggcattg aggtgcgcaa
     1381 ctcaaaatta tcgcggaatt tctggtgggt ctggtcggcg cgttcggcgt tgaaacggtc
     1441 gaattcaaat ccggtcgaaa taccgtacgt tgctgtgtca attcacgacg ggttcgccaa
     1501 ggtattccaa ggtatgatgg catcgaacga caataatgac gacgcgatcc ggtacgtttc
     1561 cgcgcccgca ggtgcggtcc gtccggccgg caccggcagc cgctgcgata agggaatccg
     1621 ccggattttt gcaggtcggt ggtaggaagc attgaaaatc cgccggaaag gtctatacat
     1681 actccacgat ccacttcgtg gacctccgct gcgatcaatt catttccatt g
//
LOCUS       DASWPO010000179         9887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2900098, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000179 DASWPO010000000
VERSION     DASWPO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2900098"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1354)
                     /locus_tag="VGC69_09505"
     CDS             complement(275..1354)
                     /locus_tag="VGC69_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEY0295552.1"
                     /translation="MRHLVRSILSCLLAAATVTLVVIPVVSLACTRVVYLGDNGDVIT
                     ARSMDWKLDITTNLYILPRGVSRTGEAGPNSLKWTAKYGSVVATGYDVSTTDGMNEKG
                     LAAELLWLVESQYPAFDKSSKPGLTIAAWAQYVLDNFATVSEAVAALEKQPFTIVTDS
                     VPGEQRLATLHLAMSDATGDSAIVEYVNGKQVIHHGRQYQVMTNSPVFDQQLALNEYW
                     KGIGGATWLPGTNRSADRFARASFYINAIPKSEDPVVALASVFSVIRNVSVPFGITTA
                     DEPNISSTRWRTVADHKRLLYFFESALTPNTFWVDLKKVDFSSGAPVKRLDLGKDQRN
                     TFNGEVSGDFRPAPPFPFLGLGAGD"
     gene            complement(1565..2341)
                     /locus_tag="VGC69_09510"
     CDS             complement(1565..2341)
                     /locus_tag="VGC69_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0295553.1"
                     /translation="MQVDMKGRTAIITGGSKGLGLAIARRYAESGANVFIVARDAAVL
                     EQARAGLAQLAPQRVGAEPADVATAAGVEAAYAAAMARFGQVDIVVNNAGISRAGAFE
                     EVTDEAWQADLDLKLFAAIRFVRLAWPQMKARRWGRVINVLNIGAKAPSAKSVPTSVS
                     RAAGMALTKALAGEGAPFNILVNALLVGRIESDQWVRRAQATGQSLETVLEEMGRSIP
                     MGRVGTADEFASAACFLASDGASYITGTALNVDGGASPVV"
     gene            complement(2461..3450)
                     /locus_tag="VGC69_09515"
     CDS             complement(2461..3450)
                     /locus_tag="VGC69_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295554.1"
                     /translation="MQPILNRLAALCAAFFLCAAPAGAASTWPSGPIRLVVPYGAGGS
                     LDVIARVLATGLTAELGRGVIVENAPGGDGTVGVHRTLLARPDGYTFLVAISSNVTLP
                     PLINASVDYHVSDLAAVAKVGTSGIVLAGRPDLKANTLPELAALSKTVKGGLTYGTLG
                     VGSMQHLSMLGVSTQAGIDALLVPYNAAAQIASDVMGGHLDLISAGLPSLVPLIQQGK
                     LKAYAVMSHDRDIGDPDIPALGEIPGLENLNFDLWTGVFAPKKTPQPIVQAMNQAIAK
                     VLAKQEVIEQYRSLGVKTAQPMTPEAFSDFVLSDEKQMRELVQKAGIGRDSAK"
     gene            complement(3500..4495)
                     /locus_tag="VGC69_09520"
     CDS             complement(3500..4495)
                     /locus_tag="VGC69_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0295555.1"
                     /translation="MLVNDPGAAPQRDEKPIRLRRMRSFFVGGGLRRVAGQPVQSFRL
                     TQGGAPRAIDVNGDHITGQMYVQEYLLEEPRHPWPILLWHGGGMSGVNWETTPDGRPG
                     WLNYFLHAGFDVYVSDAMERGRSSWSPTYPLPPIFRTLKDGWDIFRMGPVEGYATDPA
                     SRKPFPGQQFPMEAYDTFATQWVPRWAEHEAETSAAYDALLEAVGRCVVIGHSQGGGF
                     AFEAIRRRPRDVAAVVLLEPSGAPQKTEGMDAGLPPHLVVWGDFFAEHAGWRANRAVV
                     DRYGQALREHGVEVDQLDLPAMGIHGNSHFPMMDRNAGDIAALVERWIVQHARRP"
     gene            4625..5542
                     /locus_tag="VGC69_09525"
     CDS             4625..5542
                     /locus_tag="VGC69_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295556.1"
                     /translation="MQRINYPLSELRALVALHQTSSFIKAAQWQNITQSAFSRRIARL
                     EEAVGGLLVERSTRHVALTALGRELVGRIGPLLEQLDDAMTVTGSKARGESGHIVVAV
                     LISASHTIIPPALQRLRADYPGVGLHLRDDTNERVLQAVLSREAEFGVCAMAGYPPEI
                     TEQFCVEDAYVVAFPPRHPLAGRRESVSWRELQGLRVVGMSASNSNRQLIDQGLAAVG
                     IAPPWVDEVEYLSSMLGLLQDGGAVGVLPGLAVGLSQAGKLRVLPLVAPRIGRRISLI
                     RRADSSLSVVAAAFWDAVRAELLALPNIR"
     gene            5715..6026
                     /locus_tag="VGC69_09530"
     CDS             5715..6026
                     /locus_tag="VGC69_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEY0295557.1"
                     /translation="MSAKTTVTSALLASAVASIFASAAHAAPLTQAEASAALAAHKEK
                     CYGVALKGQNDCAAGPGTTCQGTSTMDFQGNSWKFVQGGTCASIQVPGGGHGSLTPIK
                     S"
     gene            6055..6978
                     /locus_tag="VGC69_09535"
     CDS             6055..6978
                     /locus_tag="VGC69_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY0295558.1"
                     /translation="MNAYAGTTTIHVPGRPRRTAPVAHVLAGTSFKHEHLAAILADPL
                     QGGFFEVHAENYMGAGGPPHRMLAAIRERYPVSLHGVCMSIGGPDALDGRHLERFRDL
                     VLRYEPVLVSEHLAWSSHGGTFFNDLLPLPYTQGTLDRVCEHIDQVQEAIRRPLLLEN
                     PSTYVAFSASTMSETDFIRAIARRTGCGLLLDINNVFVSAVNHGYSAHAYLADFPLGQ
                     IGEIHLAGHDEQRDDENERLLIDSHDRAVADAVWDLYRHVIARTGPRPTLIEWDSDLP
                     DWRLLRAQALMARRIMRADEAAPMESLDHAA"
     gene            6968..7753
                     /locus_tag="VGC69_09540"
     CDS             6968..7753
                     /locus_tag="VGC69_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY0295559.1"
                     /translation="MRPEQRPAEHEYAAAFTPGLTDPALAVPQGVVARRGKGVVRRYN
                     VYRNNVTVSLTDALAAIYPAVQRITGIEFFRAMARFHVRATPPASPLLLEYGRDFPAF
                     IESYEYARDMPWLADTARIERAWLDAYHAADLPVLAAQALAAVDPAALAGVRFRPHPA
                     TRVVRSDYPAVAIFTMNKTEGAVSPLFSSAAEDALITRPGQDVVVSRLPAGGAAFLIG
                     LIAGAPLGAAAAAAFDAAPSFDLPANLAGILSAGVFTSLQQGD"
     gene            7759..8262
                     /locus_tag="VGC69_09545"
     CDS             7759..8262
                     /locus_tag="VGC69_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0295560.1"
                     /translation="MSATQDKRHTLPGACCTALAAARRRIERLAQPWFVQLALRLALA
                     VPFWKSGILKWQGFLQLNDTAITLFTDEFQLHLPGGPYPFPAPAVFAFLSGCGEVIFP
                     VLLVLGLGTRFAAVGVLLMTCIIELTVPEGWPIHLTWAAMALGIAAWGPGRLSFDHLL
                     GDRSPRL"
     gene            8417..8989
                     /locus_tag="VGC69_09550"
     CDS             8417..8989
                     /locus_tag="VGC69_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0295561.1"
                     /translation="MTRASQNGSLESAEIRLRALLLAGLDGDAQAYHAFLQALGAHLR
                     AFLRKRLYRLPDDIEDIVQEILIAVHNGRHTYLPEQPLTAWAHAIARYKVADYLRHRS
                     RREALHEPLDDQHEIFAESDEEATQARRDVDKLLAQLPDRHRLPILHVKLQGLSVAET
                     AQLTGLSESAVKVGVHRGLKALAAKIRGTL"
     gene            8986..9627
                     /locus_tag="VGC69_09555"
     CDS             8986..9627
                     /locus_tag="VGC69_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1109 domain-containing protein"
                     /protein_id="HEY0295562.1"
                     /translation="MKTDDMISLLAANVAPVDPGALARRYRRALLLGGLGAVLLMLGL
                     FGIRPDIRAMLVTPLFWAKMAFPACVAAAALLTAARLSRPGMPVGRGWQLLAAPVAVI
                     WIAAAVTLVAAPAPERLPMILGTTWRTCPFNITLLSIPAFIALFRAMKGLAPTHLRTA
                     GAVAGLLASSVATMAYCLHCPEMGVAFWAVWYLAGMLIPAFVGAALGPALLRW"
BASE COUNT         1611 a         3303 c         3261 g         1712 t
ORIGIN      
        1 tcccccgccg caaggcgtgc cggttcgatt ccggcctcgg gcaccataag ctgtatcagt
       61 aagtccaaga tagtctcaaa acccgcagaa aataaggctc tgtgggtttt ttgtcgtccc
      121 aaacatgccg gcaccaacag ataccgtctg tcgaaggttg cggcccggca cctggcgtgt
      181 actttccggt caatcccaac ggcggaaaat actggcgccg ggactaccac ttcgcagaca
      241 agcgcaaaac ccggacgccc gggggcctat ggcgtcagtc gccggcacct agtcccagga
      301 atggaaacgg cggcgcaggt cggaagtcac ccgagacttc gccgttgaaa gtattgcgct
      361 gatccttgcc gagatcgagg cgctttacgg gcgcgcctga tgagaagtca accttcttca
      421 ggtcgaccca gaacgtgttg ggcgtcaagg ctgactcgaa gaagtagagc aagcgcttgt
      481 gatcggcgac ggtgcgccag cgggtggacg agatattcgg ctcatccgca gtggtgatgc
      541 cgaacggcac ggacacgttg cggatcacgc taaagacgct ggcaagtgcc accacggggt
      601 cttcgctttt cggaatggca ttgatgtaga acgaagcgcg agcgaatcgg tcggccgagc
      661 ggttcgtacc gggcaaccac gttgcgccgc cgatcccttt ccagtactcg ttaagggcaa
      721 gttgctggtc gaaaacgggc gagttggtca tcacctgata ctgcctgccg tggtggatga
      781 cctgcttgcc attgacgtac tcaacgatgg cgctgtcgcc agtggcatcc gacatggcga
      841 ggtgcagcgt ggcgagacgc tgttctccgg gaacactgtc cgtgacgatg gtgaaaggct
      901 gtttttcgag cgcggccacg gcctccgaaa ccgttgcgaa attgtccagg acgtactgtg
      961 cccacgcggc gatggtgagt ccgggcttgg agcttttgtc gaaagccggg tattgcgatt
     1021 cgacgagcca gagaagttcg gcagcgaggc ccttctcgtt catgccatcg gtggtggaga
     1081 cgtcgtagcc ggtcgcgacc acgctgccgt atttggcggt ccatttgagt gaattagggc
     1141 cggcctcgcc ggtacgggaa acgccgcgcg gcaggatgta gagattggtg gtgatgtcga
     1201 gcttccagtc catcgatcgg gccgtgatga catcgccatt gtcgccaaga tagacgacac
     1261 gcgtgcaggc gagcgagacg accggaatga cgaccaacgt cactgtcgca gcggcgagaa
     1321 ggcaagacag aatcgaccga acgaggtggc gcatggctag tccttactgt cgtcaaaaga
     1381 taccgctcga ttgatctccg gcgccaagca gcccaagttg accgatgcgg ctggttgtca
     1441 cgaacgagag cggccccgtg cacatggggc gccgccggca ccccgaccgc cgtggtcggc
     1501 tccggcgcgg actacaccaa ctccctgcgc tacccgcagg cccgctgctg gaatccggac
     1561 cgcgctacac caccggcgaa gccccgccgt ccacgttcag cgccgtcccg gtgatgtacg
     1621 aagccccgtc cgacgccagg aagcaggccg ccgaggcaaa ctcgtcagcc gtacccaccc
     1681 ggcccatcgg aatgctgcgg cccatttcct ccagcaccgt ctccaaagac tgcccggtgg
     1741 cctgggcgcg gcgcacccat tggtcgcttt cgatgcggcc gaccagcagg gcattgacca
     1801 ggatattgaa gggcgcgccc tcgccggcca gcgccttggt cagcgccatg ccggcggcgc
     1861 gcgacacgga ggtgggcacg gacttggccg acggcgcctt ggcgccgatg ttcagcacat
     1921 tgatgacgcg gccccagcgg cgcgccttca tctgcggcca ggccaggcgg acgaagcgga
     1981 tcgcggcgaa cagcttcagg tccaggtcgg cctgccaggc ttcgtcggtg acctcctcga
     2041 acgcgccggc gcgcgagatg ccggcgttgt tcaccacgat gtccacctgg ccgaaccggg
     2101 ccatggccgc ggcgtaggcg gcctcgacgc cggcggcggt ggccacgtcg gccggttcgg
     2161 cgccgacgcg ctgcggcgcc agttgcgcca ggccggcgcg ggcctgctcc agcaccgcgg
     2221 catcgcgcgc cacgatgaag acattggcgc cggactcggc gtagcgccgg gcgatggcca
     2281 ggcccaaacc cttgctgccg ccggtgatga tggcggtgcg ccctttcatg tcgacctgca
     2341 tggcggactc ctcgtcggtg gaacgccgcg ggcgcggtgc gcccgcgcgg catgaccagg
     2401 ccattgtatg cgcgtgccca ggccgcgctc catcccgatc cggaccgcgc ggcggcgctt
     2461 ctatttggcg ctgtcgcggc cgatgccggc cttctgcacc agctcgcgca tctgcttttc
     2521 gtcgctgagc acgaagtcgg agaaggcctc gggcgtcatg ggctgcgccg tcttgacgcc
     2581 cagggagcgg tactgctcga tgacttcctg cttggccagc actttggcga tggcctggtt
     2641 catggcctgc acgatgggct gcggggtttt cttgggcgcg aacacgccgg tccacaggtc
     2701 gaaattcagg ttttccagcc cggggatttc ccccaaggcg ggaatatcgg ggtcgccaat
     2761 gtcccggtcg tggctcatga cggcgtaggc cttgagcttg ccctgctgga tcagcggcac
     2821 cagcgacggc aggcccgccg agatgagatc caggtggccc cccatgacgt cggaggcgat
     2881 ctgcgcggcg gcgttgtagg gcacgagcag cgcgtcgatg cccgcctggg tgctgactcc
     2941 cagcatggaa agatgctgca tcgaccccac gcccagcgtg ccgtaggtca gcccgccctt
     3001 gacggtcttg ctcagggccg cgagctcggg cagcgtgttg gctttcaggt ccggccggcc
     3061 ggcgagcacg atgccgctgg tgcccacctt ggcgacggcc gccaggtccg agacgtggta
     3121 gtcgacgctg gcgttgatca ggggcggcag cgtgacgttg gacgaaatgg ccaccaggaa
     3181 ggtgtagccg tcgggccgcg ccaggagggt ccggtgcacg cccaccgtgc cgtcgccgcc
     3241 gggcgcgttt tccacgatga cgccgcggcc cagttcggcg gtcaggcccg tggccaggac
     3301 gcgggcgatg acgtccagcg aaccgcccgc gccgtacggc acgaccaggc ggatgggccc
     3361 ggacggccac gtgctggcgg cgccggcggg cgccgcgcac aggaagaagg cggcgcacag
     3421 ggcggccagc cggttcagga tcggttgcat ggtttgtctc ctcggtcttg tttgttgttt
     3481 gtcgtgtttg gggcggcggc tagggacggc gggcgtgctg cacgatccag cgctccacca
     3541 gggccgcgat gtcgccggca ttgcggtcca tcatggggaa gtgcgaattg ccatggatgc
     3601 ccatggccgg caggtcgagc tggtcgacct cgacgccgtg ttcccgcagg gcctggccgt
     3661 agcggtcgac cacggcgcgg ttggcgcgcc agcccgcgtg ctcggcgaag aagtcgcccc
     3721 agacgaccag gtgcggcggc aggccggcat ccatgccctc cgttttctgc ggcgcgccgc
     3781 tgggttccag cagcacgacc gcggccacgt cgcggggccg ccgccggatg gcctcgaacg
     3841 cgaagccgcc gccctggctg tgcccaatga cgacgcagcg gcccaccgcc tccagcagcg
     3901 cgtcgtacgc cgccgacgtc tcggcctcgt gctcggccca gcgcggcacc cactgcgtgg
     3961 cgaaggtgtc gtaggcctcc atgggaaatt gctgccccgg gaacggcttg cggctggcgg
     4021 ggtccgtggc atagccctcg accgggccca tgcggaatat gtcccaacca tccttgagcg
     4081 tgcggaagat gggcggcagg ggataggtgg gggaccagga cgagcgcccg cgctccatcg
     4141 cgtcgctgac gtagacatcg aagcccgcat gcaggaaata attcagccag ccgggccggc
     4201 cgtccggcgt ggtctcccag ttgacgccgc tcatgccgcc gccgtgccag agcaggatgg
     4261 gccagggatg gcggggctcc tccagcaggt attcctggac gtacatctgg ccggtgatgt
     4321 ggtcgccgtt gacgtcgatg gcgcgcggcg cgccgccttg cgtgaggcgg aagctttgca
     4381 ccggctggcc ggccacgcgg cgcaggccgc cgcccacgaa gaagctgcgc atgcgccgca
     4441 gccggatggg cttctcgtcg cgctgcggcg cggcgcccgg atcgttgacc aacatcgtgc
     4501 tgtctcctat ttgcctacct gcgagttccg gcgcggggac gctggcggga acggacgcgg
     4561 gccggtcagc attttctggc cactgctata ttctgtatat tgaattttgt gcgctcattg
     4621 atgcatgcag agaataaatt accccctcag cgagctgcgc gcattggtcg ccctgcacca
     4681 gacgagcagc ttcatcaagg cggcgcagtg gcagaacatc acccagtccg cgttcagccg
     4741 ccgcattgcc cggctggaag aggcggtggg agggctgctg gtggagcgat ccacgcgcca
     4801 tgtcgcgctg acggccctgg ggcgcgaact cgtcggccgg atcgggccct tgctggagca
     4861 gctcgacgac gccatgaccg tcaccgggtc gaaggcgcgg ggcgagtccg gccacatcgt
     4921 ggtggccgtg ctcatcagcg cttcgcacac gatcatcccg cccgcgctgc agcgcctgcg
     4981 cgccgattat cccggcgtgg gcctgcacct gcgggacgac acgaacgagc gcgtgctgca
     5041 ggctgtcctg tcgcgcgagg ccgagttcgg cgtgtgcgcc atggccggct atccgccgga
     5101 aatcacggag cagttctgcg tcgaggacgc ctacgtggtc gccttcccgc cccggcatcc
     5161 gctggcgggc cggcgcgagt ccgtgtcgtg gcgtgaattg caggggctac gcgtggtcgg
     5221 catgagcgcc agcaactcca accggcagct catcgaccag ggccttgccg cggtcggcat
     5281 cgcgccgccc tgggtggacg aggtggagta tctgtcgtcg atgctgggcc tgctgcagga
     5341 cggcggcgcg gtcggcgtgc tgccggggct ggccgtcggg ctgtcccagg ccggcaagct
     5401 gcgcgtgctc ccgctggtgg ccccccgcat cggccggcgc atcagcctga tccgccgcgc
     5461 cgacagcagc ctgtcggtcg tcgccgcggc gttctgggat gccgtgcgcg ccgaactgct
     5521 ggccctgccc aatatccgct gaccggcaaa acacaaaata cccgcgaaag ccttgccttg
     5581 cgcgggcgac ccacgttcga acgactccgg gcgcacaaaa aaaccaggtc cgctgtaacc
     5641 cgatctccag tccaggcgaa ttagcttatg tatccgtcgt ggataccttc gttcgcaatt
     5701 actggagaga ctcaatgtcc gcaaaaacca ccgtcacgtc cgccctgctc gccagcgccg
     5761 tcgccagcat tttcgcctcg gccgcccacg cggcgcccct gacgcaggct gaagccagcg
     5821 ccgccctcgc cgcgcacaag gaaaagtgct atggcgtcgc cctgaagggc cagaacgact
     5881 gcgccgcagg tcccggcacc acttgccagg gcacctccac catggatttc cagggcaatt
     5941 cgtggaagtt cgtccaaggc ggcacctgcg ccagcatcca ggtcccgggg ggcggccatg
     6001 gttcgctcac cccgatcaaa tcctgatccc acagaggcgg aaacggggat ggccatgaac
     6061 gcctatgccg ggacgacgac gatccatgta cccggccgtc cccgccgaac cgcgccggtt
     6121 gcgcacgtgc ttgccggaac cagtttcaag catgaacacc tggccgcgat tctcgcggat
     6181 ccgttgcagg gtggcttctt cgaagtccac gccgagaatt acatgggcgc cggcggcccg
     6241 ccgcaccgga tgcttgcggc tatccgggag cgctatccgg tctccctgca cggcgtgtgc
     6301 atgtccatcg gcggcccgga cgcactggac ggccggcacc tggaacgctt tcgcgatctg
     6361 gtgctgcgct acgagccggt gctggtatcg gaacaccttg cctggtcctc tcatggcggg
     6421 acgttcttca atgacctgct gccgctgccc tatacccaag gcacgctgga cagggtttgc
     6481 gagcacatcg atcaggtcca ggaggcgatc cggcgtccgc tgctgcttga aaatccatcg
     6541 acctacgttg cgttctcggc gtcgacgatg agcgagaccg atttcatccg ggcgatagca
     6601 aggcgcaccg gctgcggcct gctgctcgac atcaacaacg tgttcgtttc ggcggtcaat
     6661 catggctatt cggcgcacgc ctaccttgcc gattttcccc tcggacaaat cggcgaaatc
     6721 catctggccg ggcacgacga acagcgcgac gacgaaaacg aacgcctgct catagatagt
     6781 cacgaccgcg ccgtcgccga tgccgtctgg gatctctaca ggcatgtcat cgcgcgcacc
     6841 gggcccaggc cgacactgat cgaatgggac agcgacctac ccgactggcg cctgttgcgg
     6901 gcccaggcgc tgatggctcg ccggatcatg cgggcagatg aggctgcgcc gatggagagc
     6961 ctcgatcatg cggcctgaac agcgtcccgc cgagcacgaa tacgccgcgg catttacgcc
     7021 gggcctgacg gatccggcgc tcgcggtgcc gcaaggcgtg gtcgcccgcc gtggaaaagg
     7081 cgtggtccgg cgatataacg tgtaccggaa caacgtgacc gtcagcctga ccgatgcgct
     7141 ggcggccatc tatcccgcgg tccagcgcat caccggcatc gaattcttcc gggcgatggc
     7201 ccgcttccat gtccgcgcca caccgcccgc gtccccgctg ctcttagagt acgggcggga
     7261 cttccccgcc tttatcgaat cctatgaata cgcgcgcgac atgccatggc tggcggacac
     7321 ggcccgcatc gaacgggcct ggctggatgc ctatcatgct gccgatctgc cggtcctggc
     7381 agcgcaagcg ctcgcggccg tcgacccggc agcactggcc ggcgtgcgtt tcaggccgca
     7441 ccccgccaca cgggtcgttc gatcggacta tcccgcggtt gcgatcttca cgatgaacaa
     7501 gaccgaaggc gcggtgtcgc cgctcttttc aagcgcggcg gaagatgccc tgatcacccg
     7561 gcccgggcag gatgtcgtgg tgtcgcgcct gcccgcgggc ggcgcggcct tcctcatcgg
     7621 cctcatcgcg ggcgcgccgc tgggcgcggc ggcagccgcc gcgttcgacg cggccccgtc
     7681 cttcgatctt ccggccaatc tggcgggaat tctctcggcg ggtgtattta cctccctcca
     7741 acaaggagat tgaccagaat gtccgccaca caagataagc ggcacaccct cccgggcgca
     7801 tgctgcacgg cgcttgccgc cgccaggcgg cggatcgagc ggctggcgca accgtggttc
     7861 gtccagcttg cgctgcgtct cgcgctggcc gtgcctttct ggaagtcggg catccttaaa
     7921 tggcagggct tcctgcagct caacgacacg gcgataaccc tgttcaccga cgagttccag
     7981 ctgcacctgc cgggcggccc ctatcccttt cccgctcccg ccgtatttgc cttcctgtcc
     8041 ggctgcggcg aagtgatatt tcccgtgctg ctggtactgg gcctgggaac gcgcttcgcg
     8101 gccgttggcg tgctgctgat gacatgcatc atcgagctga ccgtgccgga aggctggccc
     8161 atccatttga cctgggccgc catggcgctg ggaatcgcgg catggggccc gggccgcctc
     8221 tccttcgatc atcttctggg agaccggtcg cccagactat gaatcagcca cgggaatgag
     8281 ggcccaggac ctggcatgtg cccgcggccg tcaccgccat gggcatcgat gccagacttg
     8341 cctcgaacta tctgcagtgg tggccggccg cgtagaattc cccaagaatt ttcgagcctc
     8401 gcagccggaa tcacccatga cccgtgcatc gcagaatgga tcgctggagt ccgccgaaat
     8461 caggctgaga gccctgctcc tggctgggtt ggacggcgac gcgcaagcct atcatgcctt
     8521 cctgcaagcg ttgggcgcgc acctgcgggc ttttttgcgc aagcggctct accgcctgcc
     8581 ggacgacatc gaggatatcg tccaggagat attgatcgct gtgcataacg gacgccatac
     8641 ctatcttccc gagcagccgt tgactgcctg ggcgcatgcc atcgcgcgct acaaggtggc
     8701 cgactacctg cgccaccggt cgcgccgcga agcgctgcac gaaccgctgg acgatcagca
     8761 cgagatcttc gcggaatccg acgaagaagc cacgcaagcc cggcgcgatg tcgacaaact
     8821 gctggcgcag ttgccggatc ggcaccgcct gcccatcctg cacgtcaaat tgcaaggatt
     8881 atccgtggcc gagacggccc agctcaccgg cctgtcggaa tcggcggtca aggtgggcgt
     8941 acaccgcgga ctcaaggcgc tggccgcgaa gatacgaggc acgctatgaa gacggacgac
     9001 atgatttccc tgctggccgc caatgtcgct cccgtggacc ctggcgccct ggcccggcgc
     9061 taccgccggg cgctgctgct cggcggactg ggcgcggtac tgctgatgct cgggctgttc
     9121 gggatacggc ccgatatccg tgcgatgctg gtcacgccct tgttctgggc caagatggct
     9181 ttccccgcct gcgtggccgc ggccgcactg ctgacggctg cccgcctgtc gcgtcccggc
     9241 atgcccgtgg gacgcggctg gcagttgctg gccgcaccgg tagccgtcat ctggattgcc
     9301 gcggcggtga cgctggtcgc cgcgccggcg ccggaacgcc tgcccatgat tctcggcacc
     9361 acatggcgca cctgcccatt caatatcacg ctgttgtcca tcccggcgtt tatcgcccta
     9421 ttccgggcca tgaaaggcct ggctcccaca cacttgcgta cggctggagc ggtggcggga
     9481 ctgctggcca gttccgtcgc cacgatggct tactgcctgc actgcccgga aatgggcgtg
     9541 gcgttctggg cggtctggta tctggccggc atgctgatcc cggcctttgt gggcgcggcg
     9601 cttgggccgg cattgctacg ctggtaacaa ggccaggtct cgccgcctta cctccatgca
     9661 tccatgcatc catgaggatg aggcacggca tcgccaggcg tggcagtttc tttattttgg
     9721 tataccatag gatactaaag ataccgcgcc gggcacccga caccctgaag cgcggaaaac
     9781 aacatcccat cgaggagaca aagacatgca cctgccctat cgcatcgggc tttgcgccgc
     9841 cctggcgagc ctggccttcg ctacccacac cgcggccggc gcggccg
//
LOCUS       DASWPO010000180        15323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1292236, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000180 DASWPO010000000
VERSION     DASWPO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15323
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292236"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /gene="ntrC"
                     /locus_tag="VGC69_09560"
     CDS             complement(<1..1130)
                     /gene="ntrC"
                     /locus_tag="VGC69_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY0295563.1"
                     /translation="MIKPVWIVDDDQAIRWVLEKALSRAGVATRSFFQASDVLEAMDR
                     DSPSALVSDIRMPGGNGLELLRQIKERHPALPVIVMTAFADLDSTVAAFQGGAFDYLA
                     KPFDVNEAVALIQRAMEESVGETPAPENTVDGQQGGERWMMTQSSSAAMQEVFRAIGR
                     LSQSQVTVMITGESGSGKELVARALHRHSARASGPFVAINTAAIPRDLLESELFGHER
                     GAFTGAQSMRRGRFEQADGGTLFLDEIGDMPAELQTRLLRVLSDGSFYRVGGHSPVHA
                     NVRVIAATHQPLEERVKQGLFREDLFHRLNVIRLRLPPLRERSEDIPLLARHFLARSA
                     RDLGVDTKRFTPAALAALARFPFPGNVRQLENVCHWLTVMAPA"
     gene            complement(1127..2191)
                     /gene="glnL"
                     /locus_tag="VGC69_09565"
     CDS             complement(1127..2191)
                     /gene="glnL"
                     /locus_tag="VGC69_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEY0295564.1"
                     /translation="MNVDAFDLLATSVFLVDAQGHIEYANAAAEDLFGRSRKQLCGQE
                     VARLYDDPASLKSSIDRAATGNFADVRQLSSMRRGSESVEVTVTTVALSGQPWPVMIE
                     TREIEQRMVVDRSQRLVEEIESHRELLRNLAHEVKNPLGGLRGAAQLLEAELPSPALA
                     EYTQVIISEADRLQALVDRLSGPKRQPLNAKPVNIHEICERVCALIQAEFRDGVALLR
                     DYDASAPDLLGDSARLMQAVLNVARNAAQELVLQKPGVDVPPPQITLRTRVARQVMLA
                     HRRHRLALVLSIIDNGPGVPEHIRDRIFHPLVTARPGGTGLGLSLAQDFIQQHGGLIE
                     FESRPRHTEFRLVLPLEQSH"
     gene            complement(2319..3731)
                     /gene="glnA"
                     /locus_tag="VGC69_09570"
     CDS             complement(2319..3731)
                     /gene="glnA"
                     /locus_tag="VGC69_09570"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY0295565.1"
                     /translation="MASPKDVLKTIADNEVKFVDFRFTDTIGKEQHVSVPVSVIDEDK
                     LLTGQAFDGSSIAGWKGIEASDMILIPDLETANLDPFREEPTLILTCDVIEPSDGKGY
                     ERDPRSLAKRAEAYLKSSGLGDTAYFGPEPEFFVFDSVKWGDGMSGCFVTVNSEEAPW
                     SSGLDFEGGNLGHRPKVKGGYFPVPPVDSFQDLRSEMCLVLEAMGVPVEVHHHEVAAP
                     GQLEIGTKFNTLVRRADWNQILKYVVHNVSDAYGKTATFMPKPIVGDNGSGMHVHQSI
                     WKDGQNLFAGNGYAGLSEFALYYIGGIIKHAKALNAITNPTTNSYKRLVPHYEAPVKL
                     AYSARNRSASIRIPYVGNPKGRRIETRFPDPMANPYLAFSALMMAGLDGVQNKILPGD
                     PADKNLYDLPPEEDAKIPTVCASLDQALESLDKDREFLTRGGVFTNGMLDAYIALKEA
                     DVQRLRTVTHPIEFDMYYSL"
     gene            complement(3907..5076)
                     /gene="hemW"
                     /locus_tag="VGC69_09575"
     CDS             complement(3907..5076)
                     /gene="hemW"
                     /locus_tag="VGC69_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY0295566.1"
                     /translation="MPPGESTLTSLPPLSIYVHVPWCVRKCPYCDFNSHAASDIPERA
                     YLDALRADLEQALPSVWGRQVVSVFLGGGTPSLLSAAGLDELLAMLRSHLRLLPDAEI
                     TMEANPGTAEAGRFRDYAASGVTRLSLGIQSFDDAQLQKLGRIHDAGQARAAIAMAQQ
                     AVGRVNLDLMFALPGQTPEACLADAREALSFGTEHLSLYHLTMEPNTVFAKYPPQDLP
                     DDDTAAAMQDAVEAELAAAGLQRYEVSAYARPGARCRHNLNYWEFGDYLGLGPGAHGK
                     ISFHDRIVREARARQPESWMERAGRGDGSHIAETRTLGPLDLPFEFMLNALRLKDGVA
                     ASLFAERTGLSLAAIAHQLEAGVSRGLLDADPTRLQASALGWRFLNDLQETFLPE"
     gene            complement(5227..5844)
                     /gene="rdgB"
                     /locus_tag="VGC69_09580"
     CDS             complement(5227..5844)
                     /gene="rdgB"
                     /locus_tag="VGC69_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY0295567.1"
                     /translation="MDTPNFPLRQVVLASNNPGKLREFAALFAPLGIELVPQSRLGVS
                     EAEEPHVTFVENALAKARHASRHTGLPALADDSGLCVAALGGAPGVYSARYAQRQGGE
                     KSDTANNALLVRELAGQSDRRAWYVALLVLVRSENDPRPLIGEGVWQGEIVDAPQGDH
                     GFGYDPHFYLPAQGMTAAAMPAEEKNRISHRAQALQELLVKLQMA"
     gene            5957..7753
                     /locus_tag="VGC69_09585"
     CDS             5957..7753
                     /locus_tag="VGC69_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0295568.1"
                     /translation="MPEIHATPSALAAELARIVREDQLSAHYQLVADLFHGSIHGYES
                     LTRGPQDSPLHMPSALFDTARRQGQQVALELACLRASLQGFVKSGARGKLFLNLSINA
                     VLACWHRWGADLPRHLLDDSGLPPENLIIELTEHDAAGDDMDSLLRARARLREHGIGL
                     ALDDYGTGNTNLQLWVELQPDLIKVDRYFFQRISNSASRQQLMRALMSVAQSLGTPIL
                     AEGIETAADLATVRDLGIRYAQGWHLGLVENQPQPELPEPVQALLSLDRSRRALAEGG
                     SVETLLIEAPAAHRNHHTNDDIYKLFLDFPDLHAVAVVDQAQRPVGLINRHAFSEKYA
                     MRYTRELFGRNPCTVFMTTQPVLLDVGTAIDRLAPVLASEDQRYLRDGFILTKAGRYA
                     GLGTGESLVRAVTELRLETARYANPLTSLPGNIPISRQVQLLMAGSERFIVCYGDLDH
                     FKSFNDMHGYWRGDAMIQLCAEVIKQHCQAQQNFVGHVGGDDFVMLMRSGDWRGRLER
                     LIGEFNRRAVELYDENDRNQGGIELQDRYGVPRFFPFVTLGVAAMVVHPEQCRNALPQ
                     DIATAIAELKRRVKKERASLLVDDYVAAGSPR"
     gene            complement(7726..9357)
                     /locus_tag="VGC69_09590"
     CDS             complement(7726..9357)
                     /locus_tag="VGC69_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0295569.1"
                     /translation="MRKGFKVSTALSVTLLVFVVCFALAAAAAILILRNDRQTIELLG
                     KSNIERASDLSDLNSRLFQARADLAEAKTYMESGLQEPRDAALKHADTLLAAARDSGK
                     RLMANPENDPVGRPLFERVMNTGQALNEQILTPLHKAIGGWNGIEANRLAGQLLPDAG
                     QRYVEAVDAFQAYARAQGRDAVADASSAQSRAMLGALAALALIALLALAIRLVFHRAI
                     LKPLNAAGQHFDRIADGDLTGRVAERSKNEIGVLYAAMARMQAGLGQAVASVRQGVEK
                     IHAGTAGIAEAGNDMSARSIRQAGALQEASASLAQLADTVTHNADEAGQASRQVQEVA
                     SLARQGGQAVEAAVASMRGIATASKRIAEIVGVVDSIAFQTNLLALNAAVEAARAGTQ
                     GKGFAVVAGEVRTLAQRSAQAAREIKGLIGDASQRVDMGVREVGQAGATIGRVVQSVD
                     SITAIMSHISQASLRQAEDLATVNRTVETLERHTQEDSAMVERTASQADELAGQAQSL
                     RQAVAAFKLEKHAQADPDGLPAPAGMAYLGLPAAT"
     gene            complement(9532..10257)
                     /gene="rph"
                     /locus_tag="VGC69_09595"
     CDS             complement(9532..10257)
                     /gene="rph"
                     /locus_tag="VGC69_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0295570.1"
                     /translation="MSTSTRPSGRQPDELRPFSLERGFTRYAEGSVLVKAGHTHVLCT
                     ASVLDKVPPFLKGQGQGWVTAEYGMLPRATHTRGDREAARGKQSGRTQEIQRLIGRSL
                     RAVIDLKALGERTLHLDCDVLQADGGTRCASITGAWVAAADAVALLITRGDLQASPLR
                     DAVAAVSVGLVEGRPVLDLDYQEDSACQADVNVVMTGEGAFVEVQGTGEEATFTRAQL
                     DAMLGLAERGIARLVAAQRAALA"
     gene            10514..11449
                     /locus_tag="VGC69_09600"
     CDS             10514..11449
                     /locus_tag="VGC69_09600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY0295571.1"
                     /translation="MIRSMTAFGNARADLDQGSVALELRSVNSRYLDLYFRLPDELRH
                     IETPLRELLTANLERGKVEVRASYTRNSGTELAELDPAWLERLATQLEAARQVILDVA
                     APRLVELYNWPGQRSNDALDPQVWGAACLRAARQALEQMQEARAREGARLAGMMRECA
                     DGVARIVTSVEALLPQLLADHKEKLATKLRESVDAAAPGGFAHISGPELSERLAQEAT
                     LFALRIDVAEELSRLRSHLEELQHLLSDDGKGKAAGGKRSGGSAGKRLDFLFQEMNRE
                     ANTLGSKAGSLEVTRAAMDLKLLIEQMREQAQNIE"
     gene            complement(11755..12729)
                     /locus_tag="VGC69_09605"
     CDS             complement(11755..12729)
                     /locus_tag="VGC69_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295572.1"
                     /translation="MLNRISLRQMEYFLATARHGSIAIAANQIHISPPSISAAIAHME
                     AELGVQLFVRHPSKGLTLTAVGTRVMQECEDLLERASKLYDIASDSSDTIQGVLRVGC
                     FQPLTPMIAPEVVFGFKRAFEKVDLHMVEGDQQELMQRLHNAELDVALTYDLQLEEDI
                     QFEVLSQLPPHVIVNELHPLAQQVATTLQDLASQPMVLLDLPMSRDYFISLFTEAGLE
                     PLIAARFRSEDVIRSMVANGIGYALFNVRPKSNQSLDGKRFVRVRLAGKHRPMLLGLA
                     TYAPVKQSRLAQVFMQRCRAYISDQYIPGMSAPSFFDPHIAVPPDPEP"
     gene            complement(12817..13488)
                     /locus_tag="VGC69_09610"
     CDS             complement(12817..13488)
                     /locus_tag="VGC69_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY0295573.1"
                     /translation="MTFSIIARCPGTGQFGAAVASSSPAVASRCIRARKGVGAAASQN
                     ITDPALGTMALDLMASGLAPEQTLADLQQRPFIAYRQLMAIDAHRLPAVFTGDSALGT
                     LASAVGEHAACAGNMLASRDVPAIMLSAFERASGPLAERLMQALLAGQAAGGEEGPVH
                     SAGVLVYGELDWPIVDLRLDWMERDPVQALYAAWKVYEPQVRDYVTRAHDPRSAPSFG
                     VPGDL"
     gene            complement(13520..13933)
                     /locus_tag="VGC69_09615"
     CDS             complement(13520..13933)
                     /locus_tag="VGC69_09615"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0295574.1"
                     /translation="MKHTRIRTFNTKDTYPEQKLDNDLCQVVVARGQMVFLRGQIGQN
                     LDTSENVCIGDAAGQAEQAMSNIAMLLKEAGGELQHVCKITLYIIDPRYREEVYRVVG
                     RWLKGVYPVSTGIVVSALGRPEWLVEIDATAVIPD"
     gene            complement(14074..15006)
                     /locus_tag="VGC69_09620"
     CDS             complement(14074..15006)
                     /locus_tag="VGC69_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY0295575.1"
                     /translation="MGTLVFYSAFDDFGTWKRALQAQAPALRVLHWQEADDPQAVDYA
                     LVWKPPEGFFRGMTRLKLIVNLGAGVDSLVSRADLPEGVPITRISDPQMARMMAGYVL
                     FATLRHARDIPYFEQAQRRGEWAYRHPRNPEDIRVAVLGLGELGARAASELRRQGLTV
                     LGWSRSPRQIEGVRCHAGMETLDTVLAQADILVVMLPLTPQTRGLLDRDRLSRLPRGA
                     AVVNVARGALVDQAALTELLQGGHIGSATLDVFEREPLPADDPLWRMPNVLITPHLAS
                     VAIPASAARQISNNLARVAAGQAPANRINPRLGY"
     gene            complement(15009..>15323)
                     /locus_tag="VGC69_09625"
     CDS             complement(15009..>15323)
                     /locus_tag="VGC69_09625"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY0295576.1"
                     /translation="LGLTLHFVQSNWEGQLVDWIQQARGTAGGIVINAAGLTYSSVPL
                     LDALLSFPGKIIEVHMSNIWRRERFRHHSYISKAADGVIAGLGGNGYELAIEAMSRLM
                     RA"
BASE COUNT         2534 a         4888 c         5313 g         2588 t
ORIGIN      
        1 gcaggcgcca tcaccgtcag ccagtggcaa acgttttcca actggcgaac attgcccgga
       61 aacgggaagc gagccaatgc cgccaacgcc gccggggtga accgtttggt gtccacgccc
      121 aggtcgcgcg cgctgcgtgc aagaaagtgc cgcgccagca gcgggatgtc ttccgagcgt
      181 tcgcgcaacg gcggcaggcg cagccggatc acgttcagcc ggtggaaaag gtcttcgcgg
      241 aacaggccct gcttgacgcg ctcttccagc ggctgatggg tggctgcgat gacccgtaca
      301 ttggcatgca cgggactgtg tccgcccacg cgatagaaac tgccgtcgga caacacgcgc
      361 aacagacgcg tctgcaattc ggcgggcatg tcgccgattt catcgaggaa gagcgtgccg
      421 ccatcggcct gttcgaaccg cccccgccgc atgctttgcg cgccggtgaa ggcgcctcgc
      481 tcatggccaa acagttccga ttcaagcagg tcgcgcggga tggccgcggt gttgatggcg
      541 acgaaggggc cggacgctcg cgcgctgtgc cgatgcagtg cgcgggcgac cagttccttg
      601 ccgctgccgg actcgcccgt gatcatcacg gtgacctggg actgcgacag ccgtccgatg
      661 gcgcgaaaga cttcctgcat ggcggccgag gacgactgcg tcatcatcca gcgttcgccg
      721 ccctgctggc cgtcgaccgt gttttcgggc gcgggggttt ctcccacgga ctcttccatg
      781 gcgcgctgga tcagcgccac ggcttcgttc acgtcgaaag gcttggccag gtagtcgaag
      841 gcgccgccct ggaaggccgc gacggtgctg tcgagatcgg cgaaggccgt catgacgatg
      901 accggcaagg cgggatggcg ttccttgatc tggcgcagga gctccaggcc gttgcccccg
      961 ggcatgcgga tgtcggacac cagcgccgag gggctgtccc ggtccatggc ctcgagcacg
     1021 tccgaggcct ggaagaagct gcgcgtggcc acgcccgccc gcgacagcgc cttttcaagc
     1081 acccagcgta tggcttgatc gtcgtcgacg atccagacag gcttaatcaa tgggattgct
     1141 ccaaggggag aaccaagcgg aattcggtat ggcgcgggcg cgattcgaat tcgatcaggc
     1201 cgccgtgttg ctgtatgaag tcctgggcca ggctgaggcc caggccggtc ccgcctggcc
     1261 gggcggtgac cagcggatgg aaaatgcggt cgcggatgtg ctcgggcacg cccgggccgt
     1321 tgtcgatgat ggacagcacc agcgccagcc gatggcggcg atgcgccagc atgacctggc
     1381 gggcgacgcg ggtgcgcagg gtgatctgcg gcggcggcac gtcgacgccg ggtttctgca
     1441 gcaccagttc ctgcgcggcg ttgcgcgcca cgttgagcac ggcctgcatg aggcgcgcgg
     1501 aatcgcccag caggtcgggc gccgaggcgt cgtagtcgcg cagcagggcc acgccgtcgc
     1561 ggaactcggc ctggatcagc gcgcagacgc gctcgcagat ctcgtggata ttgaccggct
     1621 tggcattgag cggctggcgc ttgggaccgc tcaggcggtc gaccagggcc tgcaggcggt
     1681 ccgcctcgga aatgatgact tgcgtgtatt cggccagggc cgggctgggc agctcggcct
     1741 ccagcaattg cgcggcgccg cgcaggccgc ccagcgggtt cttcacctcg tgggccaggt
     1801 tgcgcagcag ttcgcgatgg gattcgattt cttcgaccag gcgctggctg cggtcgacga
     1861 ccatgcgctg ttcgatttca cgggtttcga tcatgaccgg ccagggctgg cccgacaggg
     1921 ccaccgtggt cacggtgacc tcgaccgact ccgagccgcg ccgcatggac gacagttggc
     1981 gcacgtcggc gaagttgccg gtggcggccc ggtcgatgga ggatttcagc gatgccgggt
     2041 cgtcgtagag acgggcgacc tcctggccgc acaattgctt gcgggagcgg ccgaacaggt
     2101 cctcggccgc ggcattggca tattcgatat ggccctgggc gtccacgaga aagacggacg
     2161 tcgcgagcag atcaaaagca tcaacattca tcattcgaaa gtaccagggc agtttcagga
     2221 acgggcacgg aaacccactc cgcgaaacgg caaatgcatc gccagcgttt gccggcaggt
     2281 tgaaggcgcg aggcgctcca ccctgccaga ctagcgcatt acaggctgta gtacatgtcg
     2341 aactcgatcg ggtgagtcac cgtgcgcaag cgctgcacgt cggcttcctt gagtgcgatg
     2401 taggcgtcga gcatgccgtt ggtgaatacg ccgccgcgcg tcaggaactc gcggtccttg
     2461 tcgagggatt ccagcgcctg gtcgagcgag gcgcacacgg tcgggatctt ggcgtcttct
     2521 tcgggaggca gatcgtacag gttcttgtcg gcgggatcgc ccggcaggat cttgttctgc
     2581 acgccgtcca ggccggccat catcagcgcc gagaaagcca ggtaggggtt ggccatcggg
     2641 tcggggaagc gcgtttcgat gcggcggccc ttggggttgc ccacgtaggg gatgcggatc
     2701 gaggccgaac ggttgcgggc cgagtaggcc agcttgaccg gggcttcgta gtgcggcacc
     2761 aggcgcttgt acgagttggt ggtcgggttg gtgatggcgt tgagcgcctt ggcgtgcttg
     2821 atgatgccgc cgatgtagta cagcgcgaat tcggacaggc cggcgtagcc gttgcccgcg
     2881 aacaggttct ggccgtcctt ccagatcgac tggtggacgt gcatgccgga gccgttgtcg
     2941 ccgacgatgg gcttgggcat gaaggtggcg gtcttgccgt aggcgtccga gacgttgtgc
     3001 acgacgtact tcaggatctg gttccagtcg gcgcggcgga ccagggtgtt gaacttggtg
     3061 ccgatctcga gctggccggg agcggccact tcgtggtggt gcacttcgac cggcacgccc
     3121 atggcttcga gcaccaggca catttccgag cgcaggtcct ggaaggagtc gaccggcggc
     3181 acggggaaat agccgccctt gaccttggga cgatggccca ggttgccgcc ttcgaagtcc
     3241 aggccggagg accagggcgc ttcttcggag ttgaccgtga cgaagcagcc cgacatgccg
     3301 tcgccccact tcacgctgtc gaagacgaaa aattcgggtt cggggccgaa gtaggcggtg
     3361 tcgcccaggc ccgaggactt caggtaggcc tcggcgcgct tggccaggga gcgggggtcg
     3421 cgttcgtagc ccttgccgtc ggaaggttcg atgacgtcgc aggtcaggat gagggtgggc
     3481 tcttcgcgga acggatccag gttggcggtc tccagatcgg ggatgaggat catgtccgaa
     3541 gcttcgatgc ctttccagcc ggcgatcgaa gaaccgtcga aggcctggcc ggtcagcagc
     3601 ttgtcttcgt cgataacgct gacaggcacc gaaacgtgtt gttctttgcc tatggtgtcg
     3661 gtaaagcgga agtcaacgaa cttgacctcg ttgtcagcga ttgtcttcaa tacatcttta
     3721 gggcttgcca tgtcatctcc aatggtaaaa atgtcgtggg ggcatcctcc cgaccggcat
     3781 gagactgtaa gcaatatacg tgccaatgct ggtcttgggg tatccccgca gacgaagtga
     3841 aaagtcaaaa aaatgatgca atttattggt gcatttgata gtgcataatg caccaaaccg
     3901 gggcaattat tcgggcagaa aagtttcttg caggtcgttc aagaagcgcc atcccagggc
     3961 gctggcctgc aatcgggtcg gatcggcgtc cagcaagccc cgggaaacgc ctgcttccaa
     4021 ttggtgcgca atggcagcca gggacagccc ggtgcgctcg gcaaagaggc ttgccgccac
     4081 cccgtccttg agccgcaagg cattgagcat gaattcaaaa ggaaggtcaa ggggcccaag
     4141 cgtgcgggtt tcggcaatat ggctgccgtc gccccgccct gcccgttcca tccaggattc
     4201 gggctggcgg gcgcgggcct cgcgcacgat gcggtcatgg aacgagatct tgccgtgcgc
     4261 gcccggcccc aggcccaggt agtcgccgaa ttcccaataa ttgagattgt gccggcagcg
     4321 cgccccgggc ctggcgtagg ccgagacttc gtagcgctgc aggccggcgg ccgccagttc
     4381 ggcttccacg gcgtcctgca tggcggccgc ggtgtcgtcg tcgggcaggt cctgcggcgg
     4441 atatttggca aataccgtat tgggctccat cgtcaggtga tagagcgaca ggtgctcggt
     4501 gccgaaggac agggcctcgc gcgcatcggc caggcaggcc tcgggggtct ggcccggcaa
     4561 ggcgaacatc aggtccagat tgacccggcc cacggcctgc tgcgccatgg cgatggccgc
     4621 cctggcctgc ccggcatcgt ggatgcggcc cagcttctgc aattgggcgt cgtcgaagct
     4681 ctggatgccc agcgacagcc gcgtcacgcc gctggcggca tagtcgcgaa agcggccggc
     4741 ctcggccgtg cccggattgg cttccatggt gatctcggcg tcgggcaaga ggcgcaggtg
     4801 cgagcgcagc atggccagca gttcgtccag gcccgccgcc gacaacaggc tgggcgtgcc
     4861 gccgcccagg aagaccgaaa cgacctggcg gccccagacc gaaggcagcg cctgttcgag
     4921 atcggcgcgc aaggcgtcga gatacgcgcg ttcgggaatg tcgctggccg cgtgcgagtt
     4981 gaagtcgcaa tacgggcatt tgcgcacgca ccagggcacg tgcacgtaga tggacagcgg
     5041 cggcaggctg gtcagcgtcg attcgcccgg cggcatgacg acgcgattgc gggttgcctg
     5101 gccgtcggga tggagagtga aggtgcggga cattgagttc ttaagtcgcc agggctgccc
     5161 tgcgaaattc cgatgctcgg gggctgaaag gccccctgcg cttcggattg ctccggggtt
     5221 cgtggcttaa gccatttgca atttcaccag caattcttgc agcgcttggg cgcggtggct
     5281 gatgcggttt ttttcttcgg ccggcatggc ggcggcggtc atgccctggg ccggcaggta
     5341 gaagtgcggg tcgtagccga agccatggtc gccttgcggc gcatcgacga tctcgccctg
     5401 ccagacgcct tccccgatca agggccgcgg gtcgttctcc gatcgcacca gcaccagcaa
     5461 ggccacgtac caggcgcgcc ggtcggactg gccggccagc tcgcgcacca gcagcgcgtt
     5521 gttagcagta tcactctttt ctcccccctg gcgctgcgcg tagcgggcgg aatagacgcc
     5581 gggcgccccg cccagcgcgg ccacgcacag gcccgagtcg tcggccaaag cgggcagccc
     5641 ggtatgccgg ctggcatggc gggccttggc cagcgcgttt tccacgaagg tgacgtgcgg
     5701 ctcctcggct tcgctcacgc ccagccggga ttgcgggacg agttcgatgc ccaggggcgc
     5761 gaacagggcg gcgaactcgc gcagcttgcc cgggttgttg gaagccagca cgacctggcg
     5821 cagggggaaa ttcggggtgt ccatggggtg gaattgtata agccccgccg ccgccggcct
     5881 ggcgtgtgtg accaggccac aatttcgcaa cgaatttgag cgaaaatgca gggttgcttc
     5941 atagggtccc gccctcatgc ccgagatcca cgccacgccg tctgccctgg cggccgagct
     6001 cgcgcgcatt gtgcgcgaag atcagttgtc tgcgcattac caactcgtcg ccgacctgtt
     6061 ccacggcagc atccacggct acgagagctt gacgcgcgga ccgcaggaca gcccgctgca
     6121 catgccttcg gcgctgttcg acacggcgcg caggcagggg cagcaggtcg cgctggaatt
     6181 ggcctgcctg cgggccagcc tgcagggatt cgtgaaaagc ggcgcgcggg gcaagctgtt
     6241 tctcaacctc tcgatcaacg ccgtgctggc ctgctggcat cgatggggcg ccgacctgcc
     6301 ccggcacctg ctcgacgaca gcggtctgcc gcccgaaaac ctcatcatcg aactcaccga
     6361 gcacgacgcg gcgggcgacg acatggacag cctgctgcgg gcccgcgcgc gcctgcgcga
     6421 gcatggcatc ggcctggcgc tggacgacta cggcacgggc aacaccaacc tgcagctatg
     6481 ggtggaattg cagcccgacc tcatcaaggt cgaccgctat tttttccagc gcatcagcaa
     6541 cagcgcatcg cgccagcaac tgatgcgcgc gctcatgtcg gtcgcccagt cgctgggcac
     6601 gccgatcctg gccgaaggca tcgaaacggc cgccgatctc gccacggtgc gcgacctggg
     6661 catacgctat gcgcagggct ggcacctggg cctcgtggaa aaccagccgc aacccgaact
     6721 gcccgagccg gtccaggcgc tgctgagcct ggaccgttcg cggcgggccc tggccgaggg
     6781 cggcagcgtc gagacgctgc tcatcgaggc gccggcggcg catcggaacc accataccaa
     6841 tgacgacatc tacaagctgt tcctggactt tccggacctg cacgcggtgg ccgtggtgga
     6901 ccaggcgcag cggccggtgg ggctgatcaa ccggcacgcg ttctccgaaa agtacgccat
     6961 gcgctatacg cgcgagcttt tcgggcgcaa cccctgcacg gtcttcatga ccacccagcc
     7021 cgtcctgctc gacgtcggga cggccatcga ccgcctggcg ccggtgctgg catcggaaga
     7081 ccagcgctac ctgcgcgacg ggttcatcct caccaaggca ggccgctatg cgggcctggg
     7141 cacgggcgaa tcgctggtgc gcgccgtgac cgaactgcgc ctggaaaccg cgcgctacgc
     7201 caacccgctg acgtccctgc ccggcaatat tcccatcagc cggcaagtgc agctgctgat
     7261 ggccggcagc gaacggttca tcgtctgcta cggcgatctg gaccacttca agtccttcaa
     7321 cgatatgcac ggctattggc gcggcgacgc catgatccaa ttgtgcgccg aggtcatcaa
     7381 gcagcattgc caggcgcagc agaacttcgt cggccatgtg ggcggcgacg atttcgtgat
     7441 gctgatgcgc agcggcgact ggcgcggcag gctcgagcgc ctgatcggcg agttcaatcg
     7501 gcgggccgtc gaactctatg acgagaacga ccgcaaccag ggcggcatcg agctgcagga
     7561 ccggtacggc gtgccgcgct tctttccgtt cgtcacgctg ggcgtggctg ccatggtggt
     7621 gcaccccgag caatgccgca acgccctgcc gcaggacatc gcgaccgcca tcgccgagct
     7681 caagcgccgc gtcaagaaag agcgcgcgtc cctgctggtg gacgattacg ttgcggcggg
     7741 cagcccgaga taggccatgc ctgccggcgc cggcaggccg tcggggtcgg cttgcgcgtg
     7801 tttttcgagc ttgaaggcgg cgacggcttg ccgcaggctc tgggcctggc cggccagctc
     7861 gtcggcctgg ctggccgtgc gctcgaccat ggccgaatct tcctgtgtat ggcgctccag
     7921 cgtttccacc gtgcggttga cggtggcgag gtcttcggcc tggcgcaggc tggcttgcga
     7981 gatatgggac atgatggccg tgatgctgtc gaccgactgg acgacgcggc cgatggtggc
     8041 gccggcctgg ccgacttccc gcacgcccat gtcgacgcgc tggctggcgt cgccgatgag
     8101 ccccttgatt tcgcgggcgg cctgggcgct gcgctgcgcc agcgtgcgca cctcgcccgc
     8161 cacgacggca aagcccttgc cctgcgtgcc ggcgcgggcc gcctcgaccg cggcgttcag
     8221 ggccagcaga ttggtctgga aggcgatgct gtccacgacg ccgacgattt cggcgatgcg
     8281 cttcgaggcc gtggcgatgc cccgcatgct ggccacggcg gcttcgacgg cctggccgcc
     8341 ctggcgcgcc aggctggcga cctcctgcac ctggcggctg gcctggccgg cttcgtcggc
     8401 gttgtgcgtc acggtgtcgg ccaactgggc caggctggcc gaggcttcct gcaaggcgcc
     8461 ggcctgccgg atgctgcgcg ccgacatgtc gttgcccgcc tcggcgatgc cggccgtgcc
     8521 ggcgtggatt ttttcgacgc cttggcgcac cgaggcgacg gcctggccca ggccggcctg
     8581 catgcgggcc atggcggcat agagcacgcc gatctcattc ttgctgcgct cggccaccct
     8641 gcccgtcagg tcgccgtcgg cgatgcggtc gaagtgctgg ccggccgcgt tgagcggttt
     8701 caggatggcg cggtggaaca cgaggcggat ggccagggcc agcaaggcga tcagcgccag
     8761 ggcggccagc gcgcccagca tggcgcgcga ttgcgcgctg ctggcgtcgg ccacggcatc
     8821 gcggccctgc gcgcgggcat aggcctggaa ggcatcgacg gcctccacgt agcgctggcc
     8881 ggcatcgggc agcagctggc ccgcaaggcg gttggcctcg atgccgttcc agccgccgat
     8941 ggccttgtgc aggggagtca ggatctgctc gttgagcgct tgcccggtgt tcatgacgcg
     9001 ctcgaacaag gggcgcccca cgggatcgtt ctcagggttg gccatcaggc gcttgccgct
     9061 gtcccgcgcc gccgccagca gggtgtcggc gtgcttgagc gcggcgtcgc gcggctcctg
     9121 caggccgctt tccatatagg tcttggcctc ggccaggtcc gcgcgcgcct ggaacaggcg
     9181 gctgttgaga tcgctgagat cgctggcgcg ctcgatgttg gacttgccca gcagttcgat
     9241 ggtctggcgg tcgttgcgca ggatcaggat cgcggccgcg gccgcaagcg cgaaacagac
     9301 cacgaacacc agcagcgtga ccgacagggc cgtgcttacc ttgaagcctt tgcgcatttt
     9361 ctacaacctt gccgcatatt gggtccgcgc cccgcgcgcg ccatggcatg cattgtagaa
     9421 atcgactttg gcagtttctt gacacgggct gtataaaaac ccgccgccag caagtcaggc
     9481 gcagggttgg gcagggttgc atgcccgggg ctgcacggcc caggcgcgcg gtcaggccag
     9541 cgcggcccgc tgcgcggcca ccagccgcgc aatgccgcgt tcggccaggc ccagcatggc
     9601 gtcgagctgg gcgcgggtga aggtggcttc ttcgccggtg ccctgcactt ccacaaaggc
     9661 gccctcgccg gtcatgacca cattgacatc ggcctggcag gccgagtctt cctggtagtc
     9721 caggtcgagc acgggccggc cttcgaccag gccgaccgac acggccgcca cggcgtcgcg
     9781 cagggggctg gcctgcaggt cgccgcgcgt gatcagcagc gccacggcat cggcggccgc
     9841 cacccaggcg ccggtgatgc tggcgcagcg cgtgccgccg tcggcctgca ggacatcgca
     9901 atccaggtgc agggtgcgtt cgcccagggc cttgaggtcg atgacggcgc gcaggctgcg
     9961 gccgatcagg cgctggattt cctgcgtgcg gccgctttgc ttgccgcgcg ccgcttcgcg
    10021 atcgccgcgg gtatgcgtgg cgcgcggcag catgccgtac tcggccgtca cccacccctg
    10081 cccctggccc ttgagaaagg gcggcacctt gtcgagcacg ctggccgtgc acagcacgtg
    10141 agtgtgcccc gccttcacca ggacggagcc ttcggcgtag cgggtgaagc cgcgttccag
    10201 tgaaaaagga cgcagttcgt cgggctggcg gccggagggg cgtgtggagg tggacatatg
    10261 aagttcctga taattaggtc gaatgatgaa attgtacggg gtggtattct cggacgagct
    10321 tgtcgggttc tggagctttg ggttcctttg gagttcttga gacgccgagg ctgccctgcg
    10381 caattccgat gctcgggggc cgaaatgccc cctgcgcttc ggattgctcc ggggtcctcg
    10441 gctcagggca tcacagaccc tcgaccagaa catgactcat tcaatactcg gtttcttttt
    10501 caggacgcat tccatgattc gcagcatgac cgccttcggc aacgcccggg ccgatctcga
    10561 ccagggctcg gtcgcattgg aattgcgcag cgtcaacagc cgttatctgg acctgtactt
    10621 ccggctgccc gacgaattgc ggcatatcga gacgccgctg cgcgaactgc tcaccgccaa
    10681 tctggagcgc ggcaaggtcg aggtgcgcgc ctcgtacacg cgcaacagcg gcacggaact
    10741 ggccgagctc gacccggcct ggctggagcg cctggccacg cagctcgaag cggcccgcca
    10801 ggtgatcctc gacgtggccg cgccgcgcct ggtcgagctc tacaactggc cgggccagcg
    10861 cagcaacgac gcgctcgatc cccaggtctg gggcgcggcc tgcctgcgag ccgccaggca
    10921 ggcgctggaa cagatgcagg aagcccgggc ccgcgaaggc gcgcgactgg ccggcatgat
    10981 gcgcgaatgc gccgatggcg tggcgcgcat cgtgaccagc gtcgaggccc tgctgcccca
    11041 actgctggcc gatcacaagg aaaaactggc caccaagctg cgcgaatcgg tggacgccgc
    11101 cgcgccgggc ggttttgccc acatcagcgg cccggaactg tccgaacggc tggcgcagga
    11161 agccaccttg ttcgccctgc gcatcgacgt ggccgaggag ctctcgcgcc tgcgctcgca
    11221 cctcgaggag ctgcagcacc tgctgtccga cgacggcaaa ggcaaggcgg cgggcggcaa
    11281 gcgcagcggc ggcagcgccg gcaagcggct ggacttcctc ttccaggaaa tgaaccgcga
    11341 ggccaatacc ctgggttcga aggccggcag cctggaagtc acccgcgccg ccatggattt
    11401 gaagctgctc atcgagcaga tgcgcgagca ggcgcaaaat attgagtagc gtgtcaccac
    11461 aattgcccac gcaaaggccg atactcggcc ccctggccaa agcccgtctc taagggcttt
    11521 tttcatgccc caggggttgc gggaagtctc acgccaccca ggttcggcgg gcggcgcggc
    11581 gacatatccg gtccgccgag cgacggtgcg gccgatgaaa gcatcgatcc acgactgccg
    11641 cgagcgactc atgcgcggtt gatgcgaagg ctgatggaca ttgcaggcac agaatcgcca
    11701 ggcgcttgca aacaggagga aaggcggcca attcgcgcag caggccgagg cgattcaagg
    11761 ttccgggtcc gggggaacgg cgatgtgagg gtcgaagaaa ctgggcgcgc tcatcccggg
    11821 aatgtattgg tccgagatat aggcgcggca gcgctgcatg aagacctggg ccaggcgcga
    11881 ctgcttcacc ggcgcgtagg tggcgagccc cagcagcatg gggcggtgct tgcccgccag
    11941 gcgcacgcgg acgaagcgct tgccgtccag ggactggttc gatttcggcc ggacgttgaa
    12001 cagcgcatag ccgatgccgt tggccaccat ggaacggatg acgtcctccg aacggaagcg
    12061 cgccgcgatc agcggctcga ggcccgcctc ggtgaagagc gagatgaaat agtcccggct
    12121 catgggcagg tcgagcagca ccatgggctg cgaggccaag tcctgcaagg tggtcgcgac
    12181 ctgctgggcc agggggtgca gctcgttcac gatgacatgc ggcggcagtt ggctcaggac
    12241 ttcgaactgg atgtcctctt ccagctgcag atcgtaggtg agcgccacgt cgagttcggc
    12301 gttgtgcagc ctctgcatca actcctgctg gtcgccctcg accatgtgca ggtcgacttt
    12361 ctcgaacgcc cgcttgaagc cgaagacgac ttccggcgcg atcatcggag tcaagggctg
    12421 gaagcagccg acgcgcagca cgccctggat ggtgtcgctg gaatccgagg cgatgtcgta
    12481 cagcttcgag gcgcgttcca gcaggtcttc gcattcctgc atcacgcgcg tgccgacggc
    12541 ggtcagcgtc aagcctttgg acggatgccg gacgaacagc tgcacgccca gttccgcctc
    12601 catgtgggcg atggcggcgg agatggacgg cggcgagatg tgtatctggt tggccgcgat
    12661 ggcgatgctg ccatgccggg cggtagccag gaaatactcc atttggcgta gcgagatgcg
    12721 attgagcatg aggaaaaagg ggccggttcg aagtgccccc ggcatgacgc cggggttgac
    12781 gcgacaatct tcgcacgaag ccccacggcg cgccggtcaa aggtcgccgg gcacgccgaa
    12841 gctcggcgcc gagcgcggat cgtgggcgcg cgtgacgtag tcgcgcactt gcggctcgta
    12901 gactttccag gccgcgtaga gcgcctggac gggatcgcgc tccatccaat cgaggcgcaa
    12961 atcgacgatg ggccagtcca gttcgccgta gacgagcacc cccgcggagt gaaccggccc
    13021 ctcttcgccg ccggccgcct ggccggcgag cagggcctgc atgaggcgtt cggccaaagg
    13081 gccgctcgcc cgctcgaagg ccgacagcat gatggccgga acgtcgcggg acgcgagcat
    13141 attgccggcg caggcggcat gttcgccgac ggcgcttgcc agcgtgccga gcgcgctgtc
    13201 cccggtgaaa actgccggca gccgatgcgc gtcgatggcc atcagctggc ggtaggcgat
    13261 gaacgggcgc tgctgcaggt ctgcgagggt ttgctcgggg gccagcccgc ttgccatgag
    13321 gtcgagcgcc atcgttccca gggccggatc ggtgatgttc tggctggccg ccgcgccgac
    13381 gcccttgcgg gcgcggatgc agcgggacgc gacggctggc gacgaggagg cgacggccgc
    13441 gccgaattgg cccgtcccgg ggcagcgggc gatgatggag aacgtcatgg aattggcctg
    13501 gcgcgggttc ggggggatgt cagtcgggaa tgacggcggt cgcgtcgatt tccaccagcc
    13561 actcggggcg gcccagggcc gagaccacga tgccggtcga gaccggatac acgcccttga
    13621 gccaacggcc gaccacgcgg tagacctctt cgcggtagcg tgggtcgatg atgtagaggg
    13681 tgatcttgca gacgtgttgc agttcgccgc ccgcttcctt cagcagcatg gcgatgttgg
    13741 acatggcctg ttcggcctgc ccggcggcgt cgccgatgca cacgttctcg gacgtgtcca
    13801 gattctgccc gatctgcccg cgcaggaaaa ccatttgccc gcgcgccacg acgacctggc
    13861 acaagtcgtt gtccagcttc tgttcgggat aggtgtcttt ggtgttgaag gtgcggatac
    13921 gagtatgttt catattattt ccgcccgggg cggtcgaagg aggctgtgaa gaaagccctg
    13981 atgattcacc aagcgcatgg tgaaacctat ttgtcttttc cggggacgtg tctcggaatt
    14041 tccgatagcg tcccgatgac ccagcgtccc ggctcaatag cccaggcgcg gattgatccg
    14101 gttggcgggc gcttgcccgg cggcgacacg cgccaggttg ttggagatct gcctggccgc
    14161 cgaggcgggg atggccaccg acgccagatg cggcgtgatc agcacattgg gcatgcgcca
    14221 gagcgggtcg tccgccggca acggctctcg ctcgaagacg tcgagcgtcg ccgaaccgat
    14281 gtggccgcct tgcagcagtt cagtcaaagc cgcctggtcg accagcgccc cgcgggccac
    14341 attgacgacg gccgcgccgc gcggcaggcg cgacagccgg tcccggtcca gcaggccgcg
    14401 cgtctgcggc gtgagcggca gcatgaccac caggatgtcg gcctgcgcca gcaccgtgtc
    14461 cagcgtttcc atgccggcgt ggcagcgcac accctcgatt tgccgcgggc tgcgcgacca
    14521 gcccagcacg gtcaggccct ggcgccgcag ctcgctcgcg gcgcgcgcgc cgagctcgcc
    14581 caggccgagc acggccacgc ggatgtcctc gggattgcgc ggatggcgat aggcccattc
    14641 gccgcgccgc tgcgcctgct cgaaataagg aatgtcgcgc gcatggcgca gcgtggcgaa
    14701 cagcacgtag ccggccatca tgcgggccat ctgcggatcg ctgatgcgcg taatgggcac
    14761 gccctcgggc aaatccgcgc gcgacaccag cgaatccacg cccgcgccga gattgacgat
    14821 cagcttgagc cgcgtcatgc cgcgaaaaaa cccctcgggc ggtttccaca ccagcgcata
    14881 gtcgacggcc tgggggtcgt ccgcctcttg ccagtgcagc acgcgcaagg cgggcgcctg
    14941 cgcctgcagc gcgcgcttcc aggtgccgaa gtcgtcgaac gcgctgtaga agacgagcgt
    15001 gcccacggtc aggcccgcat tagccgcgac atggcctcga tggccagctc atagccgttt
    15061 ccgcccaggc ccgcgatgac gccgtcggcc gccttcgaaa tgtaggaatg gtgtcgaaag
    15121 cgctcgcgcc gccagatgtt gctcatgtgc acctcgataa tcttgccggg gaaggacagc
    15181 agggcatcga gcagcggcac cgaggaatac gtcaggccgg cggcgttgat gacgatgccg
    15241 ccggccgtgc cccgcgcctg ctgtatccag tccaccaact gcccttccca gttcgactgg
    15301 acgaaatgca gcgtcaggcc cag
//
LOCUS       DASWPO010000181         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_41712, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000181 DASWPO010000000
VERSION     DASWPO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41712"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /gene="glyS"
                     /locus_tag="VGC69_09630"
     CDS             complement(<1..1588)
                     /gene="glyS"
                     /locus_tag="VGC69_09630"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY0295577.1"
                     /translation="MSNQNLLVELFVEELPPKALRKLGDAFASVLAEQLKAQGLAGAD
                     ATLTAYASPRRLGAHLTAVAAQAADRAVSQKLMPVSVGLDAAGRPTPALLKKLAALGA
                     DASAVAGLKRAPDGKAEALFYESTAKGATLAHGLQKALDEALAKLPIPKVMTYQLADG
                     WTDVKFVRPAHGLLALHGAQVVPVTALGLASGNTTRGHRFEAAADPVVVKEADSYADT
                     LAKDGAVIAGFDARRAEIRRQLDAAAAKVGGGAKPIDDDALLDEVTGLVERPNVLVCQ
                     FEPEFLAVPQECLILTMKANQKYFPLLDAAGKLTNKFLVVSNISPADASAVTGGNERV
                     VRPRLADAKFFFDQDRKKTLASRMPGLAKVVYHNKLGTQGERVERVRSIAKSIAGAIG
                     VDAQQADQAAQLAKADLLTDMVGEFPELQGTMGRYYALNDGLAADVADAIEDHYKPRF
                     AGDELPRGQVGVVVALADKLETLAGMFGIGNLPSGDKDPFALRRHALGVIRMLIEKDL
                     PLDLDALLARYAAPAFGDKIQ"
     gene            complement(1592..2587)
                     /gene="glyQ"
                     /locus_tag="VGC69_09635"
     CDS             complement(1592..2587)
                     /gene="glyQ"
                     /locus_tag="VGC69_09635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY0295578.1"
                     /translation="MLTFQQIILTLQEYWAKQGCALLQPYDMEVGAGTSHTATFLRAI
                     GPEPWRAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPANILELYLGSLAALGF
                     DLKKNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPITGEITY
                     GLERLAMYLQGVENVYDLIWTEGAPSPGRAQDGAAPSGDSKPEAQRGGRLTYGDVYLQ
                     NEKEQSTYNFEHSDADFLFTAFAAHERQAQHLMDEQLALPAYEQVLKAAHSFNLLDAR
                     GAISVTERAAYIGRIRNLARNVAQSYLDSRARLGFPMAPKTWADETLAKLDKKAA"
     gene            2860..>3473
                     /locus_tag="VGC69_09640"
     CDS             2860..>3473
                     /locus_tag="VGC69_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY0295579.1"
                     /translation="MTTIIKEDDLIESIADGIQFISYYHPADYIRHLARAYEREESPA
                     AKDAIAQILTNSRMSAEGRRPICQDTGIVNVFMKIGMDVRFETRHTLQDLCDEGVRRG
                     YLNPDNKLRASVLADPQFERKNTKDNTPCVLNIELVPGDKVDIQIAAKGGGSENKSKM
                     AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLMA"
BASE COUNT          511 a         1142 c         1214 g          606 t
ORIGIN      
        1 cctggatctt gtcgccgaag gccggcgccg cgtaccgggc gagcagcgcg tcgagatcga
       61 gcggcaggtc tttctcgatc agcatgcgga tcacgcccag cgcgtgccgc cgcagcgcga
      121 acgggtcctt gtcgccgctg ggcaggttgc cgatgccgaa catcccggcc agcgtttcga
      181 gcttgtcggc cagcgcgacg accacgccca cctggccgcg cggcagttcg tcgcccgcga
      241 agcgcggctt gtagtggtcc tcgatcgcgt cggcgacgtc cgccgccagg ccgtcattga
      301 gggcatagta gcggcccatg gtgccttgca gctcggggaa ctcgcccacc atgtcggtca
      361 gcaggtcggc cttggcaagc tgcgcggcct ggtcggcctg ctgcgcatcg acgccgatcg
      421 ccccggcaat cgacttggcg atcgaccgca cgcgctccac gcgctcgcct tgcgtgccga
      481 gcttgttgtg atagaccacc ttcgccaggc ccggcatgcg ggacgctagc gtcttcttgc
      541 gatcctggtc gaagaagaac ttggcgtcgg ccaggcgcgg gcgcaccacg cgctcgttgc
      601 cgccggtcac ggcgctggcg tcggccgggc tgatgttgct gacgacgagg aatttgttcg
      661 tgagcttgcc cgccgcatcg agcagcggga agtacttctg gttggccttc atggtgagga
      721 tgaggcattc ctgcggcacg gcgaggaatt ccggctcgaa ctggcagacc agcacgttgg
      781 ggcgttcgac cagcccggtc acttcgtcga gcagggcgtc gtcgtcgatc ggcttggcgc
      841 cgccgccgac cttggccgcc gccgcgtcga gctgccgcct gatttccgcg cggcgcgcgt
      901 cgaagccggc gatcaccgcg ccgtctttcg ccagcgtgtc ggcatagctg tcggcttcct
      961 tgacgacgac cgggtcggca gccgcctcga agcggtggcc gcgcgtggta ttgccggacg
     1021 cgaggcccag ggccgtgacg ggcacgacct gggcgccatg cagcgcgagc aggccgtggg
     1081 cggggcgcac gaacttcacg tcggtccagc cgtcggccag ctggtaggtc atgaccttcg
     1141 ggatcggcag cttggcaagc gcctcatcca gcgccttctg caggccgtgg gccaatgtcg
     1201 cgcccttggc ggtgctttcg tagaacaggg cttcggcctt gccgtcgggc gcgcgcttga
     1261 ggccggccac ggcggaggca tccgcgccga gggcggccag tttcttgagc agcgcgggcg
     1321 tgggccggcc cgcggcatcg aggcccacgc tcacgggcat gagcttttgc gagacggcgc
     1381 ggtcggccgc ctgcgcggcg acggcagtca ggtgcgcgcc caggcggcgc ggcgaggcgt
     1441 aggcggtcag cgtggcatcc gcgccggcca gcccttgcgc cttgagttgt tcggccagca
     1501 cgctggcaaa ggcgtcgccc agcttcctga gcgccttggg cggcagttcc tcgacgaaca
     1561 gttcgaccag caggttctga ttgctcatgt ctcaggccgc cttcttgtcg agtttggcca
     1621 gcgtttcatc ggcccacgtt ttcggcgcca tcgggaaacc caggcgcgcg cggctgtcga
     1681 gatagctctg cgccacgttg cgcgcgagat tgcggatgcg gccgatgtag gcggcgcgct
     1741 cggtcacgct gatggcgccg cgcgcgtcca gcaggttgaa gctgtgcgcg gccttgagca
     1801 cctgctcgta ggcgggcagc gcgagctgct cgtccatcag gtgctgggcc tggcgctcgt
     1861 gcgcggcgaa ggcggtgaac aggaagtcgg cgtcgctgtg ctcgaagttg taggtcgact
     1921 gttctttctc gttctgcagg tagacgtcgc cataggtgag gcggcccccg cgctgcgcct
     1981 ctggcttgct gtccccggag ggggcggcgc catcctgggc gcggcccggc gatggcgcgc
     2041 cttcggtcca tatcaggtcg tagacgttct ccacgccctg caggtacatg gccaggcgct
     2101 ccagcccgta ggtgatttcg ccggtgatgg gcttgcagtc gatgccgccg acctgctgga
     2161 aataggtgaa ctgcgtgact tccatcccgt tgagccagac ttcccagccc agtccccagg
     2221 cgccgagggt ggggttttcc cagtcgtcct cgacgaagcg gatgtcgttc ttcttgagat
     2281 cgaagcccag cgccgccagg ctgcccaggt agagttccag gatattcgcc ggcgcgggct
     2341 tgagcaccac ctggtactgg tagtagtgct gcaggcggtt ggggttttcg ccgtagcggc
     2401 cgtccttggg gcggcgcgag ggctgcacgt aggcggcccg ccagggctcg gggccgatgg
     2461 cgcgcaggaa ggtggcggtg tgcgaggtgc ccgcgccgac ttccatgtcg tagggctgca
     2521 gcagggcgca gccttgcttg gcccagtatt cctggagggt gaggatgatt tgctgaaaag
     2581 taagcattga aaacctggtt ttgccggctg gatgggcggt aagccgggca ttttaactgg
     2641 gcgcaggggg agctgggttc ttgaggcgtc cattggccgg cggaaatacc ccgcgggctt
     2701 cgcagcgccg ggctcgggcc caagacgacg ggccctcggc ccttcgggct gccccggcac
     2761 gcaggcgcct gcggggcctc ccggcctcct gcgggactag gcatcgcgtc aggcgcacgt
     2821 cgtattattg aggtttttcc tccgcatccc ctgaccgcca tgaccaccat catcaaagaa
     2881 gacgacctca tcgagtcgat tgccgatggc atccagttca tcagctacta ccacccggcg
     2941 gactacatcc gtcacctggc ccgcgcctac gagcgcgagg aaagccccgc cgccaaggac
     3001 gcgatcgcgc agatcctgac caactcgcgc atgagcgccg agggccgccg ccccatctgc
     3061 caggacaccg gcatcgtcaa cgtgttcatg aagatcggca tggacgtgcg cttcgagacc
     3121 cggcacaccc tgcaggacct gtgcgacgaa ggcgtgcgcc gcggctacct caacccggac
     3181 aacaagctgc gcgcctcggt gctggccgac ccccagttcg agcgcaagaa caccaaggac
     3241 aacaccccct gcgtgctcaa tatcgagctg gtgcccggcg acaaggtcga catccagatc
     3301 gcggccaagg gcggcggttc ggagaacaag tccaagatgg ccatgctcaa tcccagcgac
     3361 tcgatcgtgg actgggtgct caagaccgtg ccgaccatgg gcgcgggctg gtgtcccccg
     3421 ggcatgctgg gcatcggcat cggcggcacc gccgagaagg ccgtgctgat ggc
//
LOCUS       DASWPO010000182         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2452675, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000182 DASWPO010000000
VERSION     DASWPO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452675"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGC69_09645"
     CDS             <1..201
                     /locus_tag="VGC69_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6492 family protein"
                     /protein_id="HEY0295580.1"
                     /translation="DSDGLFALVQSNTNIPLRQVVQKLSPYFPIKIQPYERRVDWALK
                     AAELYSAVTRRGIKMLRRARGL"
     gene            complement(230..1531)
                     /locus_tag="VGC69_09650"
     CDS             complement(230..1531)
                     /locus_tag="VGC69_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HEY0295581.1"
                     /translation="MNTTATAVSWSTPGRTTYTVLLVCWMAILFEGYDVGVMGAVLPA
                     LADYKAWALTPLQLGVLGSYALAGMFVGSFAVGMMSDMLGRRRMLLACVTLFSLTMVG
                     ASWSPTPTWFGIFRFAGGLFLGGVIPVAAALTVEYSPPERRGINYGLMYSAYSLGILC
                     AAGIAMWLMPHYGWRTVVGIGAVPIVLLPVMALLLPESLEYLLAKQDKAAALKQAQRL
                     GITSLDAFQPATAQAQSTPRDIMAAIFAPRHLRATICFWVSLFLGIMLVYGLGTWLPQ
                     IMRKNGYDLGSSLSFLMVFSLVSAVGGVILGQFADKAGTRRMVALFYVLGALGIYCLT
                     YKNTLLLNYVLVGLAGLGSISASLILTGYLANYYPSHARGAATGWALSFARIGAMSGP
                     IVGGYIGSLNLPLAWNFIIFAVAALVAGLFVMLIPKQYQAA"
     gene            1665..>2598
                     /locus_tag="VGC69_09655"
     CDS             1665..>2598
                     /locus_tag="VGC69_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0295582.1"
                     /translation="MKRFYPLMALLIGLVGALVAGPAQAAQLSPAAAAQREAYIDKLM
                     QRMTVQEKIGQLRLRSLEAGDHPNKEALLQEIRDGHVGAIFNTVIRPDIRVLQDAAMR
                     SRLKIPLFFSYDVIHGHRTIFPIGMGLASSWDLDAIAKTGRISAIEASADGLNLTFSP
                     MVDISRDPRWGRNSEGFGEDTYLTSKIGATLVHAYQGDDPAAPGHIMATVKHFALYGA
                     IEGGRDYNTVDMSPQRMFQDYLPPYKAALDAGAAGVMVALNSVNGMPATADKWLLQDL
                     LRKQWGFKGITISDHGAIMELIKHGVAADGQDAAR"
BASE COUNT          489 a          855 c          870 g          384 t
ORIGIN      
        1 gacagcgacg ggttgttcgc gctggtgcag agcaatacca atattccgct gcggcaggtg
       61 gtgcagaagc tgtcgccgta tttccccatc aagatccagc cctacgagcg ccgcgtcgac
      121 tgggccttga aggcggccga actgtattcg gccgtgacgc gccgcggaat caagatgctg
      181 cggcgcgcgc gcggcctgtg acggccgcgc ggcgggccat gcggcctggt caggcggcct
      241 ggtactgttt gggaatcagc atgacgaaca ggcccgcgac cagggccgcc acggcgaata
      301 tgatgaagtt ccacgccagc ggcaggttca ggctgcctat atagccgccc acgatagggc
      361 cgctcatggc gccgatgcgg gcgaagctca gcgcccagcc ggtggccgcg ccccgcgcgt
      421 gcgagggata ataattggcc aggtagccgg tgaggatgag cgaggccgag atgctgccca
      481 ggccggccag gcccaccagc acgtagttca gcaacagcgt gttcttgtag gtcaggcagt
      541 agatgcccag ggcgcccagc acgtagaaga gcgccaccat gcggcgcgtg ccggccttgt
      601 cggcgaactg gccgaggatc acgccgccca cggccgagac caggctgaac accatcagga
      661 acgacaggct cgagcccagg tcgtagccat tcttgcgcat gatctgcggt agccaggtgc
      721 ccaggccgta gaccagcatg atgcccagga acagcgatac ccagaagcag atcgtggcgc
      781 gcaggtggcg cggcgcgaag atggcggcca tgatgtcgcg cggcgtggac tgcgcttgcg
      841 cggtggcggg ctggaaggcg tccagcgagg tgatgcccag gcgctgcgcc tgcttgaggg
      901 cggccgcttt gtcctgcttg gccagcaggt actcgaggga ctcgggcagc agcagcgcca
      961 tcacgggcag cagcacgatc ggcacggcgc ctatgccgac caccgtgcgc cagccgtagt
     1021 gcggcatcag ccacatggcg atgcccgcgg cgcacaggat gccgagggaa taggccgaat
     1081 acatgaggcc gtagttgatg ccgcggcgtt ccggcggcga gtactcgacg gtcagcgccg
     1141 cggccacggg gatcacgccg cccaggaaca ggccgcccgc gaagcggaag atgccgaacc
     1201 aggtcggcgt cggcgaccag gacgcgccca ccatggtgag ggagaacagg gtcacgcagg
     1261 ccagcagcat gcggcggcgg cccagcatgt cgctcatcat gcccacggcg aacgagccca
     1321 cgaacatgcc ggccagcgcg tagctgccca gcacgcccag ctgcagcggc gtcagcgccc
     1381 aggccttgta gtcggccagg gcgggcagca cggcgcccat gacgccgacg tcatagcctt
     1441 cgaacaagat ggccatccag cagaccagca ggacggtata ggtggtgcgc cccggtgtgg
     1501 accaggacac ggcggtggcc gtggtgttca tgttcttccc ctgagttatg ttggtcgaag
     1561 cgtcaaacaa tttagggttg aacaatatat gtgtcaatca aatagacggg ttccagggcc
     1621 gtatcttcta gaatatgcgg tttgcccgag acatccgcag atatatgaaa cggttctacc
     1681 ccctgatggc gctgctgatc ggcctggtcg gcgcgctcgt cgccggcccc gcccaagccg
     1741 cccagctctc gccggccgcc gccgcccagc gcgaggctta catcgacaag ctcatgcagc
     1801 gcatgaccgt ccaggaaaag atcggccaac tgcgcttgcg cagcctggag gcgggcgacc
     1861 accccaataa agaggccctg ctgcaggaaa tccgcgacgg ccacgtgggg gcgattttca
     1921 atacggtgat ccggcccgat atccgcgtgc tgcaggacgc ggccatgcgg agccgcctga
     1981 agatcccgct gtttttctcg tacgacgtca tccacggcca tcgcaccata tttcccatcg
     2041 ggatgggcct ggcctccagc tgggacctgg atgccatcgc caagaccggc cgcatttcgg
     2101 ccatcgaggc cagcgccgat ggcctgaacc tgaccttctc gcccatggtc gacatctcgc
     2161 gcgatccgcg ctgggggcgc aactccgagg gtttcggcga ggacacctac ctgacctcga
     2221 agatcggcgc caccctggtg cacgcctatc agggcgacga tcccgccgcg ccgggccaca
     2281 tcatggccac ggtcaagcac ttcgcgctgt acggcgccat cgagggcggg cgcgactaca
     2341 acacggtcga catgagcccg cagcgcatgt tccaggacta cctgccgccc tacaaggcgg
     2401 cgctggacgc cggcgcggcc ggcgtgatgg tggcgctcaa ctccgtcaac ggcatgcccg
     2461 ccacggccga caaatggctg ctgcaggacc tgctgcgcaa gcagtggggc ttcaagggca
     2521 tcaccatcag cgaccacggc gccatcatgg agctgatcaa gcatggcgtg gccgccgacg
     2581 gccaggacgc cgcgcgcc
//
LOCUS       DASWPO010000183         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2097380, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000183 DASWPO010000000
VERSION     DASWPO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097380"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VGC69_09660"
     CDS             complement(<1..1353)
                     /locus_tag="VGC69_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0295583.1"
                     /translation="MFDTLLIANRGEIACRVAATARKLGIRTVAVYSDADAQAKHVAA
                     CDTAVHIGGPEPRASYLRADAILQAARDTGAQAIHPGYGFLSENGDFARAIAAAGMTF
                     VGPPASAIAAMGSKSAAKTLMEKAGVPLVPGYHGDNQDPQFLKGQADKIGYPVLIKAS
                     AGGGGKGMRVVESSDAFIEALASCQREAKSSFGDDRVLIERYLQKPRHIEIQVFADTL
                     GHCVYLFERDCSVQRRHQKVIEEAPAPGMTPERRQAMGEAAVAAAQAVGYVGAGTVEF
                     IAEPDGRFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVASGEPLPLSQEDLRIDGHA
                     IEVRLYAENPDTGFLPSIGTLAYLELPAHTAFANGAVRVDGGVRMGDAITPFYDPMVA
                     KLIVHGADRDQARRRMLAALAGTRVVGVQTNVAFLGRLMRDEAFAAADLDTGLIERRR
                     A"
     gene            complement(1365..2972)
                     /locus_tag="VGC69_09665"
     CDS             complement(1365..2972)
                     /locus_tag="VGC69_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0295584.1"
                     /translation="MPIIESRINARSQDFIDNARAMQVQIDDLAAVTAKTALGGSEAA
                     RGKHTARGKLLPRERVERLIDPGSPFLELSPLAAHGMYDGEAPGAGIITGIGRIEGTE
                     CVIVCNDATVKGGTYYPLTVKKHLRAQEIAQQNRLPCVYLVDSGGANLPRQDEVFPDR
                     DHFGRIFYNQAVMSSMGIAQIAVVMGSCTAGGAYVPAMSDESIIVRNQGTIFLGGPPL
                     VKAATGEIVSAEDLGGGDVHTRLSGVVDHLAANDLHALQLARQSVARLNLTKPQPLAL
                     APVREPRFDPAELNGIIPADTRKPFDVREIIARLVDGSEFDEFKARFGATLVTGFAHI
                     HGMPVGIVANNGILFSESAQKGAHFIELCAQRKIPLVILQNITGFMVGRKYENEGIAR
                     HGAKMVTAVATAAVPKFTVLIGGSFGAGNYGMCGRGFSPRMLFMWPNARISVMGGEQA
                     ASVLATIRREGIESKGGQWPAEEEEAFKAPIRSQYEREGHPYYATARLWDDGIIAPSD
                     TRRVLGLALSAALNAPIEDTRFGLFRM"
     gene            complement(3297..>4283)
                     /locus_tag="VGC69_09670"
     CDS             complement(3297..>4283)
                     /locus_tag="VGC69_09670"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0295585.1"
                     /translation="PARQAALNAGLPKQVACTTIHKVCGSGLKAAMFGHDLLVAGSAE
                     IVVAGGQESMSNAPYLMLKGRQGYRYGHSTIYDHMALDGLEDAYQRGTAMGVFAEDCA
                     AKYHFTREEQDAFSTESLRRARAATEDGSFKWEIAPVTVAGRKGDTVVDTDEAPTKVD
                     PAKIPTLKPAFKKDGTVTAATSASISDGAAAVVMMRASTAEQLGLKPLARVVAHAQHS
                     QEPEWFTTAPVGALKNLFAKTGWKASDVDLYEINEAFAVVTMAAMKELDLPHAKVNVN
                     GGATALGHPIGASGARLLTTLVGALKKTGGKRGVATLCIGGGEAVAMAIEMV"
BASE COUNT          585 a         1411 c         1549 g          738 t
ORIGIN      
        1 ggcgcggcgg cgttcgatca gcccggtgtc cagatcggcg gcggcgaagg cctcgtcgcg
       61 catgagccgg cccaggaagg ccacattggt ctgcacgccc accacgcgcg ttccggccag
      121 ggcggccagc atgcggcgcc gcgcctggtc gcggtcggcg ccgtgcacga tgagcttggc
      181 caccatgggg tcgtagaagg gggtaatggc atcgcccatg cgcacgccgc cgtccacgcg
      241 caccgcgccg ttggcaaagg ccgtgtgcgc cggcaactcc aggtaggcca gcgtgccgat
      301 ggagggcagg aagcccgtgt cggggttctc ggcgtacagg cgcacctcga tggcgtgccc
      361 gtcgatgcgc aagtcttcct gggagagcgg caggggttcg cccgaggcca cgcgcaattg
      421 ccactccacc aggtccaggc cggtgatcat ctcggtgacc ggatgctcga cctgcaggcg
      481 ggtgttcatt tccatgaagt agaagcggcc gtcgggttcg gcgatgaact ccaccgtgcc
      541 ggcgcccaca tagcccacgg cctgggccgc ggccacggcg gcctcgccca tggcctggcg
      601 gcgctcgggt gtcatgccgg gcgcgggggc ctcttcgatc actttctggt ggcggcgctg
      661 caccgagcag tcgcgctcga acaggtacac gcaatggccc agcgtatcgg cgaacacctg
      721 gatctcgatg tggcgcggct tctgcagata gcgttcgatg agcacgcggt cgtcgccgaa
      781 gctggacttg gcttcgcgct ggcaggaggc cagggcttcg atgaaggcat cggacgactc
      841 gaccacgcgc atgcccttgc cgccgccgcc ggcgctggcc ttgatgagca ccgggtagcc
      901 gatcttgtcg gcctggccct tgaggaactg cgggtcctgg ttgtcgccgt gatagccggg
      961 caccagcggc acgccggcct tttccatcag cgtcttggcg gccgacttgc tgcccatggc
     1021 ggcgatggcc gaggccggcg ggccgacgaa ggtcatgccg gcggcggcaa tggcccgggc
     1081 gaagtccccg ttctcggaca ggaagccgta gcccgggtgg atggcctgcg cccccgtgtc
     1141 gcgcgccgcc tgcaggatgg cgtcggcgcg caggtagctg gcgcgcggct cggggccgcc
     1201 gatatgcacg gccgtgtcgc aggcggccac gtgcttggcc tgcgcgtcgg cgtcggaata
     1261 cacggccacc gtgcggatgc ccagcttgcg ggcggtggcg gcgacgcggc aggcgatttc
     1321 gccgcggttg gcgatcaaca aggtatcaaa cattttgtct tgtcctacat ccggaacagg
     1381 ccgaagcgcg tgtcttcgat gggcgcgttg agcgcggccg acagggccag gcccagcacg
     1441 cggcgcgtgt ccgacggcgc gatgatgccg tcgtcccaca ggcgcgccgt ggcgtagtag
     1501 ggatgccctt cgcgctcgta ctgggagcgg atgggggcct tgaaggcttc ttcttcttcc
     1561 gccggccact gcccgccctt ggactcgatg ccctcgcggc gtatggtggc cagcacgctg
     1621 gccgcctgct cgccgcccat caccgagatg cgcgcgttgg gccacatgaa gagcatgcgc
     1681 ggcgagaagc cgcggccgca catgccgtag ttgcccgcgc cgaacgaacc gccgatgagc
     1741 acggtgaact tgggcacggc ggcggtggcc acggccgtga ccatcttggc gccgtggcgc
     1801 gcgatgcctt cgttctcgta cttgcggccc accatgaagc cggtgatgtt ctgcaggatc
     1861 accagcggga tcttgcgctg cgcgcacagt tcgatgaagt gcgcgccttt ctgcgccgat
     1921 tcggaaaaca ggatgccgtt gttggcgacg atgcccaccg gcatgccgtg gatgtgggcg
     1981 aagcccgtga ccagcgtggc gccgaagcgc gccttgaatt cgtcgaattc cgagccgtcg
     2041 accaggcgcg cgatgatttc gcgcacgtcg aagggcttgc gggtgtcggc ggggatgatg
     2101 ccgttgagct cggccgggtc gaagcgcggt tcgcgcacgg gcgccagggc cagcggttgc
     2161 ggcttggtga ggttcaggcg ggcgacggac tggcgcgcca gctgcagggc gtgcaggtcg
     2221 ttggcggcga ggtggtcgac cacgcccgac aaccgggtgt gcacgtcgcc gccgcccagg
     2281 tcttcggcgc tgacgatttc accggtggcg gctttgacca gcggcgggcc gccgaggaag
     2341 atggtgccct ggttgcggac gatgatggat tcgtcgctca tggcgggcac ataggcgccg
     2401 ccggcggtgc aggagcccat gacgacggcg atctgcgcga tgcccatcga ggacatgacg
     2461 gcctggttgt agaagatgcg gccgaagtgg tcgcggtcgg gaaagacttc gtcctggcgc
     2521 ggcaggttgg cgccgccgga gtccaccagg tagacgcagg gcaggcggtt ctgctgggcg
     2581 atttcttgcg cgcgcaggtg tttcttgacc gtcagggggt agtaggtgcc gcctttcacg
     2641 gtggcatcgt tgcagacgat gacgcattcg gtgccctcga tgcggccgat gccggtgatg
     2701 atgccggcgc cgggcgcctc accatcgtac atgccgtgcg cggccagggg cgaaagttcg
     2761 agaaaggggc tgcccgggtc gatgaggcgt tcgacgcgct cgcgcggcag gagcttgccg
     2821 cgggcggtgt gcttgccgcg ggcggcttcg ctgccgccca atgccgtttt ggccgtcacc
     2881 gcggccagat cgtcgatctg gacctgcatg gcacgagcgt tatcgatgaa atcctgcgac
     2941 cgcgcgttga tgcgggactc gatgatgggc atgcattacc ccgttgaggt tgtctcggtt
     3001 cgttcttgtc gggctgctgg tattgcgtgt ttactgcttt gggttcttaa ggcgtctggc
     3061 gcgcaggcgc cgggcaggcg gtgcccggcc acggaggctg actcggcccc tgaacggggc
     3121 ctcggccatc gccttcctcg tcagggcggg ccgggggcga actcgcgtcc ctgcgggccg
     3181 ctcaaacacg cccccggact tcccccgccc ttccttcgga agcgctcggc ggcctcctac
     3241 cggccgggca ccgcctgccc ggcgcctgcg cgccagactc acaacatctc agtcgctcac
     3301 accatctcga tggccatggc cacggcttcg ccgccgccga tgcacagcgt ggccacgccg
     3361 cgcttgccgc cggtcttctt cagcgcgccg accagcgtgg tcaacagccg cgcgcccgaa
     3421 gccccgatgg ggtggcccag cgcggtggcc ccgccgttca cattgacctt ggcgtgcggc
     3481 aggtcgagct ccttcatcgc ggccatggtc accacggcga acgcttcgtt gatctcgtac
     3541 aggtccacgt ccgacgcctt ccagcccgtc ttggcgaaca ggttcttcag cgcgcccacc
     3601 ggcgcggtgg tgaaccactc gggctcctgc gagtgctggg catgggccac cacccgggcc
     3661 aggggcttca gaccgagctg ctcggcggtc gaggcgcgca tcatcaccac cgcggccgcg
     3721 ccgtcggaga tcgaggccga ggtggccgcg gtcaccgtgc cgtctttctt gaaggccggc
     3781 ttgagcgtgg ggatcttggc ggggtcgacc ttggtgggcg cttcgtcggt gtcgaccacc
     3841 gtgtccccct ttctgccggc cacggtcacc ggggcgattt cccacttgaa gctgccgtcc
     3901 tcggtggccg cgcgcgcccg gcgcagcgac tcggtggaga aggcgtcctg ctcctcgcgg
     3961 gtgaagtggt acttggcggc gcaatcctcg gcgaacacgc ccatggcggt gccgcgctgg
     4021 taggcgtctt ccaggccgtc gagcgccatg tggtcataga tggtgctgtg gccgtagcgg
     4081 tagccctggc ggcccttgag catcaggtag ggcgcgttgc tcatgctttc ctggccgccg
     4141 gccaccacga tctcggccga accggccacc agcaggtcgt ggccgaacat ggcggccttc
     4201 aggcccgaac cgcacacttt atggatggtg gtgcaggcca cctgcttggg cagccccgcg
     4261 ttgagcgcgg cctggcgcgc cgg
//
LOCUS       DASWPO010000184         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1025342, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000184 DASWPO010000000
VERSION     DASWPO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025342"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGC69_09675"
     CDS             <1..298
                     /locus_tag="VGC69_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0295586.1"
                     /translation="VLALVGLAAREGHFPSQLSGGERQRVALARALVTRPACVLADEP
                     TGNLDRQTAHDVWGLLTRVNRESGTAFVIVTHDQELAARADRVLHMARGKLVAG"
     gene            320..1132
                     /locus_tag="VGC69_09680"
     CDS             320..1132
                     /locus_tag="VGC69_09680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0295587.1"
                     /translation="MLIDTHCHLDAAEFDSDRAAMIQRAREAGVQGIVIPAVGRMNFA
                     AVRDLAHAFPGGTYALGIHPMYVRHAGEGDLDLLRGLVRDALDDPRFVAIGEIGLDFF
                     VPEISQGAERARQEHFYAAQLDIAAEFKLPVLLHVRRSQDMLLKYLRRQPGVPGGIAH
                     AFNGSAQQARAFVERGFALGLGGAMTYARALQIRRHATDIGLAHLVLETDAPDIPPAW
                     LQAPDRRNAPHELARIAGVLAELRGMAREDIVHATTANACRVLPRLQALGVA"
     gene            complement(1187..2089)
                     /locus_tag="VGC69_09685"
     CDS             complement(1187..2089)
                     /locus_tag="VGC69_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295588.1"
                     /translation="MKIDSIHSLLIFMHVAQAQSFAGAALRLGITPSGASKAVSRLEA
                     KLGLRLFQRTTRHLQLTEDGTALLENCRDLFFQLEHAEEKLANRKGKPAGRLLLRMPV
                     AFGRIVIAPLLNELLETHPDLVIDIHLNDVRNDVVDDDFDVLIRIGSVPQSRLVARKL
                     YDMHYVTVASPGYVKRHGKPNRPEDLSAHHCLAYFEPLARTYRPWDFSANGRGSSVTV
                     PGSLNINNAEALLDAAVRGEGIATVARFIAAEALRLGKVTQLLPGYARPSLPIYLLYP
                     ERRYRSPRVHTLVEFLLSRVSAVD"
     gene            2196..>2747
                     /locus_tag="VGC69_09690"
     CDS             2196..>2747
                     /locus_tag="VGC69_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295589.1"
                     /translation="MIYITEMAFEDPDLLDAWHAFYLENIANLQSCPGFLASQRFESV
                     VDVIAPFTAIHEIESPEVFTSDIYRKRGGRQSNGDWQPLMTHWHRNLYAGLDATPRVA
                     PTAFLVYFDAPRAEAERELPLPSRASVNWLELVGLDRTIPHRGISVLDDASDQIAFAR
                     QDPRVKVFRPITEKISNGRPFHLY"
BASE COUNT          484 a          871 c          901 g          491 t
ORIGIN      
        1 ggtgctggcc cttgtgggcc tggcggcgcg cgagggccac ttccccagcc agctgtccgg
       61 cggcgaacgc cagcgcgtgg cgctggcgcg cgccctggtc acccgtcccg cctgcgtgct
      121 ggccgacgaa cccaccggca acctggacag gcagacggcg cacgatgtgt ggggcctcct
      181 cacgcgcgtg aaccgcgagt cgggcacggc cttcgtcatc gtgacgcacg accaggaact
      241 ggcggcgcgc gccgaccgcg tgctgcacat ggcgcgcggc aaactggtgg ccggctgaac
      301 gcgggcggag ccggatgcca tgctgatcga cacgcactgc catctggatg ccgccgagtt
      361 cgacagcgac cgcgcggcca tgatccagcg cgcccgcgag gcgggcgtgc aaggcatcgt
      421 gatcccggcc gtgggccgca tgaacttcgc cgccgtgcgc gacctggcgc atgcctttcc
      481 cggcgggacc tacgcgctgg gcatccatcc catgtatgtg cgccatgccg gcgagggcga
      541 cctggacctg ctgcgcgggc tggtgcgcga cgccctggac gacccgcgct tcgtggccat
      601 cggcgagatc gggctggatt tcttcgtgcc cgagatttca cagggcgccg aacgcgcgcg
      661 gcaggagcat ttctatgccg cgcaattgga tatcgcggcc gaattcaagc tgccggtgct
      721 gctgcacgtg cggcgctcgc aggacatgct gctcaaatac ctgcgccgcc agcccggcgt
      781 gccgggcggc atcgcgcatg ccttcaacgg cagcgcccag caggcgcggg ccttcgtcga
      841 acggggcttc gccctgggcc tgggcggcgc catgacctat gcgcgggccc tgcagatacg
      901 gcgccatgcc acggacatcg gcctggcgca cctggtgctg gagaccgacg cgccggatat
      961 tccgcccgcg tggctgcaag cgccggatcg gcgcaatgcg ccgcatgagc tggcgcggat
     1021 cgccggggtg ctggccgagt tgcgcggcat ggcgcgcgag gacatagtcc acgcgaccac
     1081 ggcgaacgcc tgccgtgtcc tgcctcgctt gcaggccttg ggcgtggcat aagggtattg
     1141 cgcgagtcca caggcatcac tgggccgagg ttttgcggag ggcacatcaa tccacggctg
     1201 agacacggga cagcagaaac tccaccaaag tgtgcacccg gggcgagcgg tagcggcgct
     1261 cggggtagag caagtaaata ggcaggctgg gccgcgcgta gcccggcaag agttgcgtca
     1321 cctttcccag gcgcaacgcc tcagccgcga tgaaccgggc aacggtcgcg atgccttctc
     1381 cgcgaacggc cgcatcgagt agcgcttccg cattattgat gttcagcgaa cccggtaccg
     1441 tgaccgacga accgcgtccg ttcgccgaga aatcccaagg gcgatatgtc ctggccaggg
     1501 gttcgaaata cgcgaggcaa tggtgggcgg ataagtcctc ggggcggttg ggcttgccgt
     1561 ggcgcttcac gtagccgggc gaagcgaccg tcacatagtg catgtcgtac agcttgcgcg
     1621 ccaccagtct cgattgcggc acggaaccaa ttcggatcaa gacgtcgaag tcgtcatcca
     1681 ccacatcgtt gcgaacatcg ttgagatgta tgtcgatcac caggtcgggg tgcgtctcca
     1741 ggagttcgtt cagcagcggc gcgatcacga tccggccgaa ggcgacgggc atgcgcagca
     1801 gcaggcggcc ggccggcttg cccttgcggt tggcaagctt ttcttccgcg tgctcgagtt
     1861 gaaagaacaa gtcgcggcaa ttctccagca gcgccgtgcc atcttccgtc aattgcagat
     1921 gacgggtcgt ccgctgaaac aggcgcagcc ccaacttggc ttccagccgg cttaccgcct
     1981 tgcttgcgcc cgatggcgtg atgcccaagc gcagcgccgc gccggcaaag ctctgagctt
     2041 gcgccacgtg catgaatatc agcagggagt gtatggaatc gattttcatt catgacattg
     2101 tgtcattaac tgttggacat tgcatgtctg ttttgcgcat tcgaaaaaac ctatcctgaa
     2161 attcccttgg cgactggaaa aggaatttct tctcgatgat ttacatcact gaaatggcat
     2221 tcgaggaccc ggacctgctg gatgcctggc atgcgttcta cctggagaac atcgcgaatt
     2281 tgcagagctg cccgggcttt ctggcatcgc agcgtttcga gtccgtggtg gatgtcattg
     2341 ctccgttcac cgccatccac gagatcgagt cgcccgaggt cttcaccagc gatatatacc
     2401 ggaaacgggg cggccgtcag tccaatggcg actggcagcc gctgatgacg cattggcatc
     2461 ggaatctcta cgccgggcta gacgcgaccc ccagggttgc gccaaccgct ttccttgtct
     2521 acttcgatgc gccacgggcc gaagcggaac gggaactgcc cctgccatcc agggcgtcgg
     2581 tgaactggct ggaactcgtg ggcctggatc gcacgattcc gcatcgcggc attagcgtgc
     2641 tggatgacgc gtcggatcag atcgcgttcg cacggcagga cccgcgcgtg aaggtattca
     2701 gacccatcac ggagaagatc agcaatggac ggccgtttca tctttac
//
LOCUS       DASWPO010000185         4229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1739659, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000185 DASWPO010000000
VERSION     DASWPO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739659"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VGC69_09695"
     CDS             complement(<1..1393)
                     /locus_tag="VGC69_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY0295590.1"
                     /translation="MSDAFQPEWAFPYTSQRMPVIAEHVACTEQPLATQIALSIMAAG
                     GNAVDAAIAAGIALAVVNPCMNGIGGDIFAIVWDGQRLHGLNASGRSPAAWTPGHFAH
                     YDKMPYRGWDTVTVPGTVSGWVALSRRFGKLPFAELFKPAIRYAREGYLVTPIVSRQW
                     QAQIGQIIGTPGFAEAFAPNGRAPLPGERFICPGQADTLEAIARTQGEDFYRGDLAAR
                     LVRYAQETGGTMTASDLAEHQADWVEPISMDYGELALHEIGPSGQGIGALMALGMLDA
                     AGASKQALDSIDYFHYQIEAMKLAFADINRYVADPAHMDKVTAAHLLDRDYLRKRAGD
                     IDPARAQFPGPGTPHDGGTTYLTTADAGGMMVSFIQSNFKGFGSGLVVPDSGIALHNR
                     GWGFNLIPGHPNQVGPRKRPFHTIIPGFIMRGGRPLMSFGVMGGSLQAQGHLQVTARL
                     ARGQNPQAIIDAPR"
     gene            complement(1418..2416)
                     /locus_tag="VGC69_09700"
     CDS             complement(1418..2416)
                     /locus_tag="VGC69_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295591.1"
                     /translation="MIAFRRGRRMLRAMPGALLALGLACAAGQALGADTFPDHAIKLI
                     VPVPPAGAADTSARAIGQFISARLGQQIIIDNRPGAGTTLGLRMAAHAPADGYTLTLV
                     AVSGIAIATVSYNDLPDMRKDFVTVAGIVDAPHMLVASAALGVHSVPELVGLLAKNPN
                     RYNYASQGTGSLSHLESAVFVDARHLQVQHIPYAGSSAAMPGLISGTTAMMMDSVASV
                     LPQARAGKLIVLGTTARRRVPQFPDAPTMGELGVPGLVADNPFVILAPAGTPPERVKV
                     LTDALAAALADPQVDKTLADAGLVPHFVAPADFAKIFDQEYGLWPGVARKLTGPAS"
     gene            2566..3171
                     /locus_tag="VGC69_09705"
     CDS             2566..3171
                     /locus_tag="VGC69_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEY0295592.1"
                     /translation="MDEDVIKAMARWPDVPAAYGWLSLDARGRWRLHPAGDSAAGGPG
                     EAIAHAGVLAFMNRNYAREGPDWYVQNGPQRAYARLDAAPYLLRRADAGPGLQTHTGL
                     AVGRLEHWWLDEEGHLYAQTDLGPGMVEDRELSPLLERMRTAAGEPVLLVLEGWLEDV
                     RAALPALSLDGGPPAPLARLARADVPGHLGFHTNPSPRTGG"
     gene            3182..3763
                     /locus_tag="VGC69_09710"
     CDS             3182..3763
                     /locus_tag="VGC69_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0295593.1"
                     /translation="MTAPAPIRYFPAPRTARFCSQCGSPIRRGIPDDDNRERDICDHC
                     GAIHYQNPRIVVGTLPRWQGKVLLCKRAIEPRYDTWTLPAGFMELNESTVQGAERETV
                     EEAGARIELGQLYTMVDLPHIGQVHLFYLADALGPELDPGPESLEARYFDLAEIPWGD
                     LSFHSVSSTLRHYVEDSRKGVFPVRQYTLNTPL"
     gene            3779..>4229
                     /locus_tag="VGC69_09715"
     CDS             3779..>4229
                     /locus_tag="VGC69_09715"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY0295594.1"
                     /translation="MKLAWLDLDSPFPPVGMALDDPPGLLAAGADLSLPRLTLAYRSG
                     IFPWYAQGEPILWWSTHPRMVLACADFAPSHSLRKRLRRIEAESAGPAPAVAVKVDTA
                     FDAVLAGCAAPRDGQSGTWILPAMQQAYRAWHAAGAVHSIETWMDGEL"
BASE COUNT          643 a         1493 c         1460 g          633 t
ORIGIN      
        1 agcgcggcgc gtcgatgatg gcctgcgggt tctggccgcg cgccagccgc gccgtcacct
       61 gcaggtggcc ctgggcttgc agcgagccgc ccatcacgcc aaagctcatc agcggccggc
      121 cgccgcgcat gatgaaaccc ggaatgatgg tgtggaatgg gcgcttgcgc gggcccacct
      181 ggttggggtg tcccgggatc aggttgaagc cccagccccg gttatgcagc gcgatgccgg
      241 aatcgggcac caccagcccc gagccgaagc ccttgaaatt ggactggatg aacgacacca
      301 tcatgccccc ggcatcggcc gtggtcaggt aggtggtgcc gccgtcgtgc ggcgtgccgg
      361 ggccgggaaa ctgcgcgcgc gccgggtcga tgtcgcctgc gcgcttgcgc aaatagtcgc
      421 ggtcgagcag atgcgccgcc gtgaccttgt ccatatgggc cgggtcggcc acatagcggt
      481 tgatgtcggc gaaggccagc ttcatggctt cgatctggta gtggaagtag tcgatcgaat
      541 ccagcgcctg cttggaggcg cccgcggcgt cgagcatgcc cagggccatc agtgcgccga
      601 taccctgccc gctggggccg atctcgtgca gcgccagttc gccgtagtcc atggagatcg
      661 gctcgaccca gtcggcctgg tgctcggcca ggtccgaggc ggtcatcgtg ccgccggtct
      721 cctgcgcata gcgcacgaga cgcgccgcca ggtcgccacg gtagaaatcc tcgccctggg
      781 tgcgggcgat ggcctccagc gtgtcggcct ggcccgggca gatgaagcgc tcgcccggca
      841 ggggcgcccg gccattgggc gcgaaggcct cggcgaagcc tggcgtgccg atgatctgcc
      901 cgatctgcgc ctgccactgc cggctgacga tgggcgtgac cagatagccc tcgcgcgcgt
      961 agcggatggc gggtttgaac agctcggcga acggcagctt gccgaagcgc ctggacagcg
     1021 ccacccagcc cgagaccgtg cccggcacgg tgaccgtgtc ccagccgcga tagggcatct
     1081 tgtcgtagtg ggcaaaatgc ccgggcgtcc aggcggccgg cgagcggccc gacgcgttca
     1141 ggccatgcag gcgctggccg tcccagacga tggcgaaaat gtcgccgccg atgccattca
     1201 tgcacgggtt gaccacggcc agtgcgatgc ccgcggcaat cgcggcatcg accgcgttgc
     1261 cgccggccgc catgatgctc aacgcgatct gcgtggccag cggctgttcg gtgcaggcga
     1321 catgctcggc gatcaccggc atgcgctgcg aggtgtaggg gaaggcccat tcgggctgga
     1381 aagcatcgga catggaacga tcctggacaa aaggcgatta agaagccggg ccggtcagct
     1441 tgcgcgccac gccgggccac aagccatatt cctggtcgaa gatcttggcg aagtcggccg
     1501 gcgcgacgaa atgcggcacc aagccggcat cggccagcgt cttgtcgacc tgcggatcgg
     1561 ccagcgcggc ggccagcgca tcggtgagca ccttgacacg ttccggcggc gtgccggcgg
     1621 gcgccaggat cacgaacgga ttgtcggcca ccagccccgg aacgccgagc tctcccatgg
     1681 tgggcgcatc ggggaactgc ggcacgcggc ggcgcgccgt ggtgcccagc acgatgagct
     1741 tgccggcgcg ggcctgcggc agcacggagg ccacgctgtc catcatcatc gcggtggtgc
     1801 cgctgatcag ccccggcatg gcggccgagc tgcccgcgta cggaatgtgc tgcacctgca
     1861 ggtgccgggc gtcgacgaac accgccgact ccaggtgcga caacgacccg gtgccctgcg
     1921 acgcatagtt gtagcggttg ggattcttgg cgagcagccc gacgagttcg ggcacgctgt
     1981 gcacgcccag ggccgccgag gccaccagca tgtgcggcgc gtcgacgatg ccggccacgg
     2041 tgacgaaatc cttgcgcatg tccggcaggt cgttgtagct gacggtcgcg atggcgatgc
     2101 ccgacaccgc caccagggtg agcgtgtagc cgtcggccgg cgcgtgggcc gccattctca
     2161 ggcccagcgt ggtgcccgcg ccgggacggt tgtcgatgat gatctgctgg ccgagccggg
     2221 ccgagatgaa ctggccgatg gcgcgcgcgc tggtgtcggc cgcgccggcg ggcggcacgg
     2281 gcacgatcag cttgatggca tggtcgggaa aggtatcggc gcccagcgcc tgcccggcag
     2341 cgcaggccag gcccagcgcc agcaaggccc ctggcatcgc gcgcagcatg cggcggcccc
     2401 gtcgaaaagc aatcatggca ttccccttgg atttttttgc tcgctacccg ggcgggccac
     2461 acaggcatgg ctccagcgga gcgcggcggt gcatcgatca taggaattga tagttgttgc
     2521 gtcaaccaat taatcgggcg tgaggtctgc ggctatattg gccgcatgga cgaagacgtg
     2581 atcaaggcca tggcccgctg gcccgatgtg ccggcagcct acggctggct ctcgctggac
     2641 gcgcgcggcc gctggcggct gcaccccgcg ggcgactcgg cggcgggcgg cccgggcgag
     2701 gccatcgccc atgccggcgt gctggccttc atgaaccgca actatgcccg ggaaggcccg
     2761 gactggtatg tgcagaacgg cccgcagcgc gcctacgcgc ggctggatgc cgcgccctat
     2821 ctgctgcgca gggccgatgc cggcccggga ctgcagaccc ataccggcct ggccgtgggc
     2881 cggctggaac actggtggct ggatgaagaa ggccatctgt acgcccagac cgacctgggg
     2941 cccggcatgg tcgaggaccg tgaactgtcc ccgctgctgg agcgcatgcg gaccgcggcg
     3001 ggcgagcccg tcctgctcgt cctggaaggc tggctggagg acgtgcgcgc cgccttgccg
     3061 gccctctcgc tcgacggcgg cccgcccgcg ccgctggccc gcctggcccg cgccgacgtg
     3121 cccggccatt tgggatttca caccaacccg tcgccccgga cgggcggtta gaatcttccg
     3181 catgactgct cccgcgccca tccgctattt cccggcaccc cgcactgccc ggttctgctc
     3241 ccaatgcggc tcgcccatcc ggcgcggcat acccgacgac gacaaccgcg aacgcgacat
     3301 ctgcgaccat tgcggcgcca tccactacca gaacccgcgc atcgtggtgg gcaccctgcc
     3361 gcgctggcag ggcaaggtgc tgctgtgcaa gcgcgccatc gagccgcgct acgacacctg
     3421 gaccctgccc gccggcttca tggagctcaa tgaaagcacc gtgcagggcg ccgagcgcga
     3481 aacggtggaa gaggccggcg cgcgcatcga gctcggccag ctctacacca tggtggacct
     3541 gccgcacatc ggccaggtgc acctgttcta cctggccgat gccttgggcc ccgaactcga
     3601 ccccggcccc gaaagcctgg aggcgcgcta cttcgacctg gccgagattc cctggggcga
     3661 cctgtccttc cattcggtgt cgtccaccct gcgccactat gtcgaagaca gcaggaaggg
     3721 cgtgttcccc gttcggcaat acacgctgaa cacgcccctc tgagtcccgc ctgacgcctt
     3781 gaaactcgcc tggctcgacc tcgactcgcc cttcccgccc gtgggcatgg cgctcgacga
     3841 cccgcccggg ctgctggccg ccggcgccga cctgtccctc ccccgcctga ccctggccta
     3901 ccgcagcggc atcttcccct ggtacgcgca aggcgaaccc atcctctggt ggagcacgca
     3961 cccgcgcatg gtcctggcct gcgcggactt cgccccctcg cactcgctgc gcaagcgcct
     4021 gcgccgcatc gaggccgaat cggccggccc cgcccccgcg gtcgcggtca aggtcgacac
     4081 cgccttcgac gccgtgctgg ccggctgcgc cgcgccgcgc gacggccagt ccggcacctg
     4141 gatactgccc gccatgcagc aggcctaccg cgcctggcac gccgccggcg ccgtgcacag
     4201 catcgaaacc tggatggacg gcgagctgg
//
LOCUS       DASWPO010000186         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1382988, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000186 DASWPO010000000
VERSION     DASWPO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382988"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /gene="hpnE"
                     /locus_tag="VGC69_09720"
     CDS             complement(<1..1123)
                     /gene="hpnE"
                     /locus_tag="VGC69_09720"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEY0295595.1"
                     /translation="MKAAVIGAGWAGLAAATALHEAGAHVTVFEAGRTPGGRARRVVQ
                     PGFDSVLDNGQHILLGAYAQTLALMRRLGRNPDTLLMRRPLRLASLDGRFRLSAPSLP
                     PPWHGAAALLTARGLGWRAKLAALRFVRSLQASGWNPPPVWTVAELLRYRQQPADLVD
                     CLWTPLCLSALNTPPEQASATLFARVLKDTLAGKLRDSDLLLPCVDLSALWPDAATLH
                     LTMRYHSTVRQLHTDAGSVEINGERFEAAVLAVPPAIVVRLLEPALQAQGAHELLQTL
                     QSFDYQPIATLNLKLAQPWPLPEPMLMLREDRARGHLGQWLFDRSRLTGRSRQAELAV
                     VASAAQTLADTPKETAIAALVEQIREQAADGGLPALPAVE"
     gene            complement(1120..1986)
                     /gene="hpnD"
                     /locus_tag="VGC69_09725"
     CDS             complement(1120..1986)
                     /gene="hpnD"
                     /locus_tag="VGC69_09725"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY0295596.1"
                     /translation="MTPDDYCQDKAAKSGSSFYYAFLFLPSERRRAITALYAFCREAD
                     DVVDEAREESVARIKLAWWRTQIDKMFDGAPDHPVTRALQPHLQAFGITRERLLAIID
                     GMEMDLDQSRYLDWPGLRKYCWLAAGVVGELSAGIFGYTDPRSLEYAGKLGVAFQLTR
                     IIRDVGDDARRGRIYLPVDDLQQFQVKAADILNGVYSERFTALMRFQAERARGLYREA
                     VGLLPEADRRAQRPGLIMAAIHHDLLAEIERDGWQVLHQRISLTPMRKLWLAWKTWVG
                     GGRGLIRRLARA"
     gene            complement(1988..2890)
                     /gene="hpnC"
                     /locus_tag="VGC69_09730"
     CDS             complement(1988..2890)
                     /gene="hpnC"
                     /locus_tag="VGC69_09730"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY0295597.1"
                     /translation="MAVDHYENFPVASLLLPCRLRPAVRAIYRYARSADDIADEGDAS
                     DDERLADLARYRHELQRIGTGQVPGDGSRPLHAAPFGLAASQEGEAALGRPGADLAPV
                     FTPLAAVIAQHQLPLVPFFDLLSAFEQDVTVKRYESYASLLDYCAHSANPIGRLMLAL
                     YGAATPDNLRDADAICTGLQLVNCWQDIRADWRRGRVYLPQEDLRRHGVAEEDLAVGR
                     LTPQWRALIAFETERAAALLHSGAALAGRLPGRIALELRLVIQGGLRILRRIEQTDYD
                     VFLNRPELRAQDWAVMLWRALLRY"
     gene            2936..>3825
                     /gene="alr"
                     /locus_tag="VGC69_09735"
     CDS             2936..>3825
                     /gene="alr"
                     /locus_tag="VGC69_09735"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0295598.1"
                     /translation="MPRPISASISIPALRHNLSVVRRHLDQAAQAAGGQAASIWAVIK
                     ANAYGHGIERAVAGFSAAQGLAMLDLDEAVRCREAGWGGPILLLEGFFKAADLELVDR
                     YHLTVAVHGDEQFDMIEAARLTRRLDIQLKLNTGMNRLGYAPSAYAAAHARAELLQRR
                     GVLGSVIKMTHFATADGPEGIAGALGVFTQATQGLAGGVSICNSAATLRYPEASVSRD
                     GSPQWVRPGVCLYGASPFADVPAASYGLRPAMTLSSEIIGVQSVSAGGAIGYGATFRA
                     ERRMRVGVVACGYADGYPRHA"
BASE COUNT          552 a         1272 c         1421 g          580 t
ORIGIN      
        1 gctctacagc gggcagggcg ggcaggccgc cgtccgcggc ctgttcgcgg atctgttcga
       61 ccagcgcggc gatggccgtt tccttgggcg tgtcggccag ggtctgcgcg gcgctggcca
      121 ccacggccag ttcggcctgg cggctgcggc cggtaaggcg cgagcggtcg aaaagccatt
      181 gccccaggtg gccgcgcgcg cggtcttcgc gcagcatcag catgggttcg ggcaggggcc
      241 agggctgcgc cagcttgagg ttcagcgtgg cgatgggctg gtagtcgaag ctctgcaggg
      301 tctgcagcag ttcatgcgcg ccttgcgcct gcagggcggg ttccagcagg cggacgacga
      361 tcgccggcgg gacggccagc acggccgcct cgaagcgttc gccgttgatt tccaccgagc
      421 ccgcatcggt atgcaattgg cgcaccgtgc tgtggtatcg catggtcagg tgcagcgtgg
      481 cggcatccgg ccacagggcc gacaggtcca cgcagggcag cagcaggtcg ctgtcgcgca
      541 gcttgccggc cagcgtgtcc ttgagcacgc gggcgaacag cgtggcgctg gcttgttcgg
      601 gcggcgtgtt caaggcggac aggcagagcg gcgtccagag gcagtcgacc aggtcggccg
      661 gctgttgccg gtagcgcagc agttcggcca cggtccagac ggggggcggg ttccagccgc
      721 tggcctgcag ggaacgcacg aagcgcagcg ccgccagctt ggcgcgccag cccaggccgc
      781 gcgcggtcag cagcgcggcg gcgccatgcc agggcggcgg cagggacggg gccgacagcc
      841 ggaagcggcc gtccaggctg gccagccgca aggggcggcg catcaggagc gtatcggggt
      901 tgcggcccag gcgccgcatc agcgccaggg tttgcgcata ggcgcccagc aggatgtgct
      961 ggccgttgtc cagcaccgag tcgaagcccg gttgcaccac gcggcgcgcg cggccgcccg
     1021 gcgtgcggcc ggcctcgaac acggtgacgt gcgcgccggc ttcgtgcagg gccgtggcgg
     1081 cggccaggcc ggcccagccg gcgccaatga cggcggcttt catgcgcgcg ccaggcggcg
     1141 tatcaggccg cggccgccgc ccacccaggt tttccaggcc agccagagct tgcgcatggg
     1201 cgtgagcgag atgcgctggt gcagcacctg ccagccgtcg cgctcgatct cggccagcag
     1261 gtcgtgatgg atggccgcca tgatcaggcc cggacgctgg gcgcggcggt cggcctcggg
     1321 cagcagcccg acggcttcgc ggtagagccc gcgggcgcgt tcggcctgaa agcgcatcag
     1381 cgccgtgaag cgttcggaat acacgccgtt gagtatgtcg gcggccttga cctggaactg
     1441 ctgcagatcg tcgacgggca ggtagatgcg gccgcggcgg gcatcgtcgc ccacgtcgcg
     1501 gatgatgcgg gtgagctgga aggccacgcc cagcttgccc gcgtattcca gcgagcgcgg
     1561 gtcggtgtag ccgaagatgc ccgccgacag ttcgcccacc acgccggccg ccagccagca
     1621 gtatttgcgc aggccgggcc agtccaggta gcggctctgg tccaggtcca tttccatgcc
     1681 gtcgatgatg gccagcagcc gttcgcgcgt gatgccgaag gcctgcaggt gcggctgcag
     1741 ggcgcgggtg acggggtggt cgggcgcgcc gtcgaacatc ttgtcgatct gcgtgcgcca
     1801 ccaggccagc ttgatgcggg ccacggattc ttcgcgggct tcgtcgacca cgtcgtccgc
     1861 ctcgcggcag aaggcataca gcgcggtgat ggcgcggcgg cgctcggacg gtaggaacag
     1921 gaaggcgtag tagaagctcg aaccgctctt ggcggccttg tcctggcagt agtcgtcagg
     1981 ggtcatttca atatctgagt agtgctcgcc acagcatgac ggcccagtcc tgggcacgca
     2041 gttcgggccg gttcaggaac acgtcgtaat cggtttgctc gatgcgtcgc aggatgcgca
     2101 ggccgccctg gatgaccagg cgcagctcca gcgcgatgcg gcccggcagt cggccggcga
     2161 gcgctgcgcc agagtgtagc agcgccgcgg cgcgttccgt ctcgaaggcg atgagggcgc
     2221 gccattgcgg ggtgagccgg cccacggcca ggtcttcctc ggcgacgccg tgccggcgca
     2281 ggtcttcctg cggcaggtag acgcggccgc ggcgccagtc cgcgcggatg tcctgccagc
     2341 agttcaccag ttgcaggccg gtgcagatcg catcggcatc gcgcaggttg tcgggcgtgg
     2401 cggcgccgta cagggccagc atcagccggc cgatggggtt ggccgagtgc gcgcagtagt
     2461 cgagcaggga cgcgtagctc tcgtagcgct tgacggtgac gtcctgctcg aaggccgaga
     2521 gcaggtcgaa gaacggcacg agcggcagct ggtgctgcgc gatgacggcg gccagggggg
     2581 taaaaacggg cgccaggtcg gcgccgggcc gccccaaggc ggcctcgccc tcttgggagg
     2641 cagcaagccc gaagggcgcg gcgtggaggg gcctgcttcc gtcgccgggc acctggccgg
     2701 tgccgatgcg ctgcagttca tggcggtagc gcgccaggtc cgccaagcgc tcgtcgtcgc
     2761 tggcatcgcc ttcgtcggcg atatcgtcgg cactgcgcgc gtagcggtag atcgcgcgca
     2821 cggccgggcg caacctgcag ggcagcaaca gcgatgcaac agggaaattc tcgtaatgat
     2881 cgacagccat ggggaaatca tatgtcaggt agactagcgg cacaggaatt ccaccatgcc
     2941 gcgtcccatc tccgcatcga tttccattcc cgccctgcgc cacaacctgt ccgtcgtacg
     3001 ccgtcatctc gaccaggccg cgcaggcggc gggcggccag gccgcgtcca tctgggccgt
     3061 catcaaggcc aatgcctacg gccacggcat cgagcgggcc gtggccggat tctcggccgc
     3121 gcagggcctg gccatgctcg acctcgacga ggccgtgcgc tgccgcgagg ccggctgggg
     3181 cggtcccatc ctgctgctcg aaggcttctt caaggccgcc gatctcgagc tcgtcgaccg
     3241 ctaccacctc acggtggccg tgcacggcga cgagcagttc gacatgatcg aggcggcgcg
     3301 cctcacccgg cgcctcgaca tccagctcaa gctcaacacc ggcatgaatc gcctgggcta
     3361 tgcgccgagc gcctatgcgg ccgcgcacgc tcgcgctgaa ctcctgcagc ggcgcggcgt
     3421 gctgggttcg gtcatcaaga tgacgcactt cgccacggcc gacggtccgg aaggcatcgc
     3481 cggcgcgctg ggcgtattca cgcaggccac gcaggggctg gcgggcggcg tcagcatttg
     3541 caattcggcc gccacgctgc gttatcctga agcctcggta agccgcgacg ggtcgccgca
     3601 atgggtgcgc ccgggcgtct gcctgtacgg cgcgtcgccg ttcgccgatg ttcccgcggc
     3661 gtcctacggc ttgcgtcccg ccatgacgct gagctccgag atcatcggcg tgcagtcggt
     3721 gtccgcgggc ggcgccatcg gttatggcgc caccttccgc gccgagcggc gcatgcgcgt
     3781 gggcgtcgtg gcctgcggct atgccgacgg ctatccgcgc catgc
//
LOCUS       DASWPO010000187        31240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1293243, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000187 DASWPO010000000
VERSION     DASWPO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 31240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31240
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293243"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VGC69_09740"
     CDS             <1..227
                     /locus_tag="VGC69_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295599.1"
                     /translation="GLAAQLEAQMQTVVDRYECEWANALQDPEKLKRFRTFVNAQGGD
                     PDIRFVQERGQIRPADPRPARVIPIAEELL"
     gene            227..586
                     /gene="nirD"
                     /locus_tag="VGC69_09745"
     CDS             227..586
                     /gene="nirD"
                     /locus_tag="VGC69_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEY0295600.1"
                     /translation="MPGAETPGIQPAWRAVCSRRDLVANSGVVALVDGVQVALFYLPQ
                     AEGPTLYAIENRDPKSGANVIGRGIVGHLGGSLVVASPLYKQHFRLEDGACLEYPEQR
                     LRTWKARLNADTVELAL"
     gene            838..2142
                     /locus_tag="VGC69_09750"
     CDS             838..2142
                     /locus_tag="VGC69_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HEY0295601.1"
                     /translation="MARNRMPATLSFLLAARRCELHDMEDLARTCELVILVGDLVHAL
                     QKERGYSNLFLRHAQPQMAPVLAALQEEALQAEIKTRGLLGGLVPDEALPIMGKTRLF
                     NCIAYALHRLDEAMDLRWRVRERRIESQEATAQFTRLIASLLAVVFEAADTALDPDVT
                     RTLVALFNFMQGKELSGQERALGVVGYAAGYFTDAQKARMRELAESQGRCFGIFAEYA
                     PPDALQRWEQTQYDSAPVQRMRAMAQQTSPEQAIDGGLSELWYDIATARIDAMRAVAM
                     LLAQGLARLCRQRIADTREELDNHRLLMSRFTDQAGSETPTQLFNVQARPIDAPLQDG
                     LASGTERAMLDIMREQRLRMDRYDADLARARSALDERKTIEQAKWLLVHHRKLSEQAA
                     HTLLQQTAMKNSLSLAEVARRVLSEISDADQAQDGADTPAGH"
     gene            complement(2145..3155)
                     /locus_tag="VGC69_09755"
     CDS             complement(2145..3155)
                     /locus_tag="VGC69_09755"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HEY0295602.1"
                     /translation="MPDQARALPRIALLATGGTIAGAQAQANTATYKAGALSVANLVD
                     AVPALAGLARIQAEQIANIGSQNMSHAVWAALARRIDELDGDDAVHGIVITHGTDTLE
                     ETAYFLSLVARTEKPLVLVGAMRPATALGAEGPANLYNAVALAASAAARDRGPLVVMN
                     EDIHYAREVQKIASMGLCAFASPNRGRAGVMHGGQPHFYSRNTTRHTRDSEFTLAALE
                     TAGWPRVDIVHAHAGMDGLVIDALAGHAAGIVLAGVGDGNAADAALAALSRAAAAGVA
                     VVRASRTGSGLVARGVEVDDAGLGFVAAGELNPQKARVLLMLALSASRDPAQWQRCFE
                     QY"
     gene            complement(3148..4062)
                     /locus_tag="VGC69_09760"
     CDS             complement(3148..4062)
                     /locus_tag="VGC69_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY0295603.1"
                     /translation="MATKKPKGLGRGLDVLLGADAPAVDNLGKAASQKEGPPSVLPVS
                     KLRAGKYQPRTRMDEGALAELAESIRTQGIMQPILVRGLGEATPGEYEIIAGERRFRA
                     AQMAGLKEVPVLVRDVPDENAAVMALIENIQREDLNPLEEAQGVRRLLDEFGLTHEQA
                     AQAIGRSRSATSNLLRLLNLAAAVQTMLLAGDIDMGHARSLLSVDAATQIQLANQVVA
                     KRLSVREAEKLVARTQKEAETATAKKGKSVSRDVTRLEEALSDHLGTRVSLKIGAKEK
                     GQIVIDFHGWEHLNSLLERQGLSGVLDA"
     gene            complement(4102..4893)
                     /locus_tag="VGC69_09765"
     CDS             complement(4102..4893)
                     /locus_tag="VGC69_09765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0295604.1"
                     /translation="MQENRKTPGAVDVTRIEEASLNATVVPEQQLYHGWLVRWAQSSA
                     KRARSVSILSPSPVALDERLAYVNGIYARHGLPLLFRFTDACPDAALEPALHARGFKA
                     YGNTLVMAVSMQAAADAAARQGQGALQLRVADTRTFAAQVGRLKGSSPEYIAEHALRL
                     ASVAMPKQALLAYDGTDCVGAALGVFDGGLMGIFDVISDAARRRQGIAAQLLQAQLQE
                     GLRQGARQAYLQVEAGNQAARTLYARYGFTEHHQYWYRSLAGAQQ"
     gene            complement(4896..5708)
                     /locus_tag="VGC69_09770"
     CDS             complement(4896..5708)
                     /locus_tag="VGC69_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0295605.1"
                     /translation="MTASASQSKPSALVFCIANQKGGVGKTTTAINLAAGLATQGQRV
                     LLVDLDPQGNATMGSGIDKSTLNATLYQVLIHEATITDARVRSEVGGYDVLPANRDLA
                     GAEIDLVGMEERERQLKQAISSVADQYDFILIDCPPTLSLLTLNGLVAAHGVIIPMQC
                     EYFALEGLSDLVNTIKRVHRNINGELQLIGLLRVMFDPRMTLQQQVSAQLESHFGDKV
                     FKTVVPRNVRLAEAPSYGMPGVVYDRSSRGAQAYIAFGLEMIERVKAMKVES"
     gene            complement(5757..6449)
                     /gene="rsmG"
                     /locus_tag="VGC69_09775"
     CDS             complement(5757..6449)
                     /gene="rsmG"
                     /locus_tag="VGC69_09775"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY0295606.1"
                     /translation="MNQAVATSADFARRLTAACQQLGLRASDAQRDKLLRYVEQMQRW
                     NRTYNLTAVRDAGQMLVQHVFDSLAVARPIENETADNETAPRPLRIMDVGSGGGLPGV
                     VLAILHDRWRVQCVDAVDKKMAFVRQISGALALPNLQASHARVETLPQAGCDVVVSRA
                     FASLLDFANLAGLHVHEKGSLLAMKGKTPEDEIAALHAQGVWRVRRIETLHVPELDAQ
                     RCLVWMRRTGQA"
     gene            complement(6446..8365)
                     /gene="mnmG"
                     /locus_tag="VGC69_09780"
     CDS             complement(6446..8365)
                     /gene="mnmG"
                     /locus_tag="VGC69_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY0295607.1"
                     /translation="MDFPREFDVIVVGGGHAGTEAALASARTGATTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAIATDEAGIQFRILNSSKGPAVRATRAQADRV
                     LYRKAIRGRLENQANLWLFQQAVDDLMVEGDKVVGAVTQIGLKFRARTVVLTAGTFLN
                     GLIHVGLQNYSGGRAGDPPAISLGARLKELKLPQGRLKTGTPPRIDGRTIDYSALEEQ
                     PGDLNPIPVFSFMGNAAMHPRQLPCWITHTNSRTHDIIRGGLDRSPMYSGVIEGVGPR
                     YCPSIEDKIHRFADKDSHQVFLEPEGLDTHEVYPNGVSTSLPFDVQLDLIHSLPGLEQ
                     AHILRPGYAIEYDYFDPRELKSTLETKAISGLFFAGQINGTTGYEEAAAQGLLAGVNA
                     ALQAQGKEAWTPRRDEAYLGVLVDDLVTRGVTEPYRMFTSRAEYRLSLREDNADLRLT
                     EIGRKLGLVDDARWDAFSRKRDAVAQELERLKSTWVNPRLMPAETAEPLLGKAIEREY
                     SLSDLLKRPNVSYAKLMAARLGDGSLLAGPGLSDDEVLAEQIEIQTKYAGYITRQQDE
                     VRKHVEHEQQSIPADIDYDAVTSLSFEVRQKLKAHKPETIGQASRISGVTPAAISLLL
                     IHLKRLHYGARKQAA"
     gene            complement(8911..9114)
                     /locus_tag="VGC69_09785"
     CDS             complement(8911..9114)
                     /locus_tag="VGC69_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0295608.1"
                     /translation="MAKGKVKWFNADKGYGFITPDSGGTDVFAHFSAIQGRGYRSLNE
                     GQEVEFEVKDGPKGPQAAEIKPL"
     gene            9385..10551
                     /locus_tag="VGC69_09790"
     CDS             9385..10551
                     /locus_tag="VGC69_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0295609.1"
                     /translation="MLQSKLPDVGTTIFTVMSRLAQEHQAINLGQGFPDFNPDPVLCQ
                     LVAKAMAEGHNQYPYMTGVPPLRHAIARKVQALYGRAYDPDTEITVTSGATEALMATV
                     LAVVGTGDEVVVIEPCYDSYLPAIRLAGGIPVPVPMQAPTREDPCFRVDWQRVRDAMT
                     PRTRLIMMNFPHNPTGIVLQEQDLDALESIVRDTGVLLLSDEPYEHIIFDGQPHRSLA
                     TRPLLADHAFVISSFGKTYHATGWKVGYCCAPKALTAELRKIHQFIVFTVSSPMQFAL
                     AEYMQDPKPYLELPAFYQAKRDRLATGLADSRFKVLPNPGTFFLLANYSDISRQSEAD
                     FSRWLTVEHGVTVIPVSAFYRDPDAPQSNHQLVRFCFAKRESTLDAAIEKLQKV"
     gene            11186..12124
                     /locus_tag="VGC69_09795"
     CDS             11186..12124
                     /locus_tag="VGC69_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY0295610.1"
                     /translation="MKLDLLVLVPLDDENKASLANHFEIHHVPDKKDQAPQQLAQHAQ
                     VRAILTNGTVGCTAAQLAALPKVELVCALGAGYENIDLEAMRQRGITVGNGAGTNDNC
                     VADHAMGLLLAAVRHIVQLDPYTRAGGWRDALGPLSNVSGKRMGILGMGIIGRQIAKR
                     GLGFDLEIGYHNRRQIEGEPYAYFGSAKELAAWADYLIVVTPGGPTTRHLVNAEVLEA
                     LGPRGFVVNVARGSVVDTAALAAALKGGVIAGAGLDVYESEPKPPQALLELENVVLTP
                     HVAGNSPEAIAASYQRFIDNAARYFAGQPLISPVPL"
     gene            complement(12226..13236)
                     /locus_tag="VGC69_09800"
     CDS             complement(12226..13236)
                     /locus_tag="VGC69_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295611.1"
                     /translation="MISDVVSSLTRPSRRHVGKALLALGLAAALPGMAMAEDAYPSHP
                     VTIVVPFPPGGATDISARIVAEGLSKKWNQSVIVVNKPGAGGNVGSEYVAHTKPDGYT
                     LLLGVTGSHGINVSLYPNLPYDPIKDFTPLTMATLYPNAIAVNPSVPAKNLQELIALL
                     KKDGNKYSYGSDGNGTASHLGLEILKQRGQFPITHVPYKGSAPMLTDLMAGQIQVGIT
                     GLPAILPGAQSGKLRLIAITTADRFPGLDYPTVAEQGFPGYAAAPWSGFFAPKGLSPE
                     LTAKISADMREVMSDPEAQKKILTVGSQFVPSTPEAFHDFLVDEIKKWGDAVKISGAK
                     ID"
     gene            complement(13302..14192)
                     /locus_tag="VGC69_09805"
     CDS             complement(13302..14192)
                     /locus_tag="VGC69_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY0295612.1"
                     /translation="MKATSQFRALIKSGDFIVSPGVYDGYSIRLVEAAGFKTACTSGA
                     AISNALLGIPDIGVMGLTENVNHCRNLARSVSIPLTADADNGYGNPVNVFHTVRMFEE
                     AGVAGINLEDQVSPKRCGHMPGKDVIDIKEAVKKIEAACEARRDDDFVIIARTDSLAI
                     EGIEGAIKRAKAYAAAGADMLFPDAVHSEDEIKRIVDAAGIPVSINMGFGIRKRPTTP
                     LIPLPRLKELGVRRISLPRMLPASAIHAMQQALEVMKEVIATGEPVHRPDVAVGIEDI
                     MNLMGYEAMRALEKRLTTIE"
     gene            complement(14245..14928)
                     /locus_tag="VGC69_09810"
     CDS             complement(14245..14928)
                     /locus_tag="VGC69_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0295613.1"
                     /translation="MDIQESTKADLDDNDAPLADVVYQHLLDAIYQGQLPPGSVVNEL
                     ALAQEFGISRGPVREAVRRLQGIQLITRQPYAKSRVISLSTESAIELFELRMALEGMA
                     CSLATRRMNDVDIEQLLHELESHHHPRSDPTPKVFDFHERIVRACGNTRIINALCGDL
                     YHLLRIYRRHSTSVVIERKEDAYEEHWQILHAMKARNAALAESLMRSHIGRATDNLAK
                     QLSTSSLSR"
     gene            15044..17755
                     /gene="acnA"
                     /locus_tag="VGC69_09815"
     CDS             15044..17755
                     /gene="acnA"
                     /locus_tag="VGC69_09815"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY0295614.1"
                     /translation="MHQYQRTQTSRFSMQRTLDCPAGAYQYVDISDVSRRFGRAVETL
                     PYVHRVLLENLARSQAWGLPVAENEIRALLAQDQGADLPLYVGRVILPDSSGLPVLQD
                     LAALRDAVARAGGDASAVEARIPVDLIVDHSLQVDHWGDSQAVMLNLGREFQRNGERY
                     RFLKWAQQAFRRVHVFPPGTGIIHQVNLERIATVVSAREQNGAQWAFPDFVIGGDSHT
                     PTVNALGVLAWGVGGIDAEAAMLGQAYTFPRPNVVGVRLVGSIVPPALTTDAALLITQ
                     RLREIGVAGDMVEYFGPAVAGLTVQERATIANMSPEYGATCGFFAVDEQTLRYLRDSG
                     RSDAQVELVKAYCQANHFWRGEDAPLPEYDRVVEIDLSEAAPSMAGPRRPQDRLVLGA
                     ISGDFRGRLAKPLSEGGFGAKPLAGTKDRLKHGDIVLAAITSCTNTSNPVVMLAAGLV
                     ARKAMEHGLRVPSWVKTSMAPGSRVVTQYLQRAGLLAPLETLGFAVIGYGCTTCGGKS
                     GPLDPGVAEQITRNDLVVASVLSGNRNFEGRIHKLIRANYIGAPPMVVIYALAGRIDI
                     DFAAEPVGHDAAGKPVFLRDLWPTPDEIAQLLPLANDHDLFNEIYDGESDGAGPWTAL
                     EAPQGERFAWDPKSSYLVEPPFFHDASGGNAIAKLADSLKQARVLAAFGDSLTTDHIS
                     PSGEIPAETPAGQYLQSLGVAPKDFNTFVGRRGNFEVMTRGTFANIRIKNVLLPQEEG
                     GYTRHFPDDVRMTIFDAAAAYRQAGVASIILGGREYGTGSSRDWAAKGSALLGVRAVI
                     AESYERIHRANLVGMGVLPLAFMPGQGWRQLGLDGSESFCFERVEDAVRSGGTVLVIA
                     QGKSGEIRFEAQPQVLTEAERGLMAAGGIPAHVLQSFLSRAEGPAAA"
     gene            17779..19140
                     /locus_tag="VGC69_09820"
     CDS             17779..19140
                     /locus_tag="VGC69_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0295615.1"
                     /translation="MSLDLLLTNARLMLPQRGLTPGVVGVKDGRIAVIADKASGLSAQ
                     DTIDCGGLWVLPGVIDPHVHFGFGSPETDFATESKTAAIGGTTTVLSFHRSKDIRESA
                     DAARDLGESQSCIDFGFHFGITSHLHVQTLEEISRRFGVSSYKLYMMYKGAAGLSKGF
                     TDIDDGLLYAALQQVAAIPGAVLGVHCENVEVIPVLREPLRAAGRDDLKAWNEQSPDF
                     LEAENVHRVCYFASRTRTPVNIVHLSCKEALDEARRHRLKKAAPIYVEGCPHYLFLND
                     ESPAGTYAKVNPPVRSQADVEAMWEGVIDGSITTLGSDHVPRKRVTKDKDIWTASNGF
                     PGTGMMLPIMLHEGYHKRGVPLEKIVELLSYNSSRLYNMPAKGSISVGCDADLLIVDP
                     DLQRTVDPAELESNADYSPYEGMTLKGWPVRTLVRGRTIMQDGRITDDARRQPGGRFI
                     RRL"
     gene            19197..19922
                     /locus_tag="VGC69_09825"
     CDS             19197..19922
                     /locus_tag="VGC69_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0295616.1"
                     /translation="MSETKTKRIWDDFLTERDQEVLKFAGYGKRGGFGKRPALFIIDV
                     QYNFTGDTPEDILDGLKTYRTHCGREAWAAVEHIIPLLELAREKNMPVFYTESGRRPD
                     LIDSGVQVGKNHRGGEKTVIAGTHATQTVEPLAPRPQDFLISKQKPSCFFGTTFMSQL
                     NFLDVDTLIMVGCTSSGCLRATTVDAYSYNFKCIIPEEAAFDRFQASHAVNLFDLNCK
                     YADVIPSIEVVEYLRNQPQFVRP"
     gene            19947..20957
                     /locus_tag="VGC69_09830"
     CDS             19947..20957
                     /locus_tag="VGC69_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0295617.1"
                     /translation="MSQAPPLPVYLLTGFLGSGKTTLLARLIHQPAFADTAVIINEFG
                     DVGLDHVLLEKSDDRDVVLLESGCLCCASNSPLQDSLEDLYYRRLRGDIPAFSRVVVE
                     TSGLADPLPLMNAINASATVSRQYRFAGVVTVVDAVNGAASLREYRECAVQLAVADRV
                     VLTKTDVASAAETEAVRGLIAQANPQADVLPVTLDSEIDLLRGLEMREHAPVAPVAGQ
                     AAGKFSHLLRYGITSHVLRVNGPVGWPRYAAWVGTAQRVLGERLLRAKGILEFEDGEV
                     CAIHGVRHLFGTPRPLSGPVPAPQCGAVVLITSDVDEEDIETVARALGATVMPQAVLA
                     AM"
     gene            20985..22154
                     /locus_tag="VGC69_09835"
     CDS             20985..22154
                     /locus_tag="VGC69_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0295618.1"
                     /translation="MSNTDVIVAGAGPVGLVAAVVLAQAGLKVALLEKRDQLNQASKA
                     STFHPPTLAIIDRLGVFGIMESHAQHVGSQQYRTTERGVIGNLSYDQLQGLTTHPFRK
                     HLEQSRLTPLLLEKFKTFPGAQAHFNTELVGIDANDDSGVTATVLQDGQQKQLKARYL
                     LGTDGAHSNVREAAGIAFDARPYPGRVLRARAEPASLDRILPGMAAITYLVNDSHSAS
                     FLRMPDCWRIILRVPAGTSDETAMADDWIFERLHALAPAMRALPTLVGRDVYGASKAM
                     AAQNRAGNVYLCGDAAHLTNTRGGMNMNCGIHDSYTLARAMVRAIQDGDAAGLHAAAA
                     ERLRVVSEVLLPRTDSMVSQEGTWIERVSKLLNEPDHARDYLYKSAMLDMVDLSV"
     gene            22192..22929
                     /locus_tag="VGC69_09840"
     CDS             22192..22929
                     /locus_tag="VGC69_09840"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295619.1"
                     /translation="MKNALPMLPSPEYRLGVLVPPANPTVEIEYPALVPAGVALHAMR
                     LPVIPGDLDARNRGYVPAYAECIKGFGSLKLDAIAVAMTGPQYRLLFDGDIDLCKRLS
                     DAAGIPVETASMALYNALKALGVERLSLLSPYPDWLTTLAAAYWQSAGFQVDHIRAFE
                     DKLVAYTVTPAQVAEALRDTRAEPGGAVVMSGTGMRTLEALAQVQAKGGTPILSSNLC
                     SVWSLVRNVKDMTSAPWMSAALPYALR"
     gene            23004..23999
                     /locus_tag="VGC69_09845"
     CDS             23004..23999
                     /locus_tag="VGC69_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295620.1"
                     /translation="MPKQQTSGLRAALAAFAAVGLMAGAGAHAEQPVPGYPSKSIRMI
                     VPFPAGGTTDVVARLIAKGITTAWGQSVVIDNRGGAAGMIGSAEGARAAPDGYTLVMG
                     NNQTHATNASLFAKPQFDITNGVTPIAMLTRTKHVIVVRSDSPIKTYQDLIAAGKDHH
                     LSFGSPSAGSSSHIISDAIRRATGMDATHIPYKGAAPLMVDLLSGQIDFSTATYGSAA
                     SYIHDGKLRPLAVGAEQRDPELPNVPTFDELGLKGLSLYSWVGLYAPANTPEPIVRAW
                     SDAVARILKDPDVISTLKTAGFEVWCKPYTEMKTFHPEEIKRWADLVKAGGISLD"
     gene            complement(24365..25594)
                     /locus_tag="VGC69_09850"
     CDS             complement(24365..25594)
                     /locus_tag="VGC69_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295621.1"
                     /translation="MNNDEFLNLACLRAVREVAETGSVSRAAMALFRAQSAVTRAVQE
                     VEAMLGAPLFDRRPTGMLPTPVCGTVLKRSERVFDELDALARWCAARQSRWQAVSDGG
                     APAYLLNTRRLQILTALARHRHMPSAARALGITQPAVSSAIRILENGAGMLLFHRNPR
                     GLLPTNDGETFLLHVRRALNELRHVSDDVAALRGSIQGVVTVGALPLGRTLILPQAVA
                     RVIGRYPGVRVVTDESAYEPLVAGLRAGDIDFVLGALRENAAASGLENEKLMSENLAI
                     LARRGHPLAGVRGLTLKDLTHAQWILSRAHSPARGLFDALFRRVKIAPPMPAVETADL
                     AVIRGLLLDTDMITVLSAQQLHYELASGQLVALDVTLRKVRRDIGLTLRAGGTPSPAA
                     RVLIDAIREVAGEVAGR"
     gene            25682..26707
                     /locus_tag="VGC69_09855"
     CDS             25682..26707
                     /locus_tag="VGC69_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0295622.1"
                     /translation="MIIDIHGHYTTAPKALEAWRNRQVAAIGSPSEMPKAAELRISDD
                     ELRESVETNQLRLMRERGLDLTIFSPRASFMAHHIGDFQVSSTWAAICNELCWRVSQL
                     FPDNFVPAAMLPQSPGVDIATCIPELVKCVEQYGNVAINLNPDPSGGHWTSPPLSDRY
                     WYPIYEKMVEYDIPAMIHVSTSCNACFHTTGAHYLNADTTAFMQCLTSDLFHDFPTLR
                     FVIPHGGGAVPYHWGRFRGLAQEMKKPLLKDHLLNNIFFDTCVYHQPGINLLTSVIPV
                     DNILFASEMIGAVRGIDPETGHYFDDTKRYIETAHLDPDERYKIYEGNARRVYPRLDA
                     RLKRNGH"
     gene            26774..27457
                     /locus_tag="VGC69_09860"
     CDS             26774..27457
                     /locus_tag="VGC69_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY0295623.1"
                     /translation="MSTTLLRVLEQPLPRVDDALLKRYAEVGCAQISDCMNRLYGVVS
                     LAPYHDTKGPRMVGIALTVKARPGDNLFIHKAITLARPGDVIVVDGGGDLTNALVGEL
                     MAMEAERRGVRGFVIDGSVRDADVFAKNGNFGCFARGVSHRGPYKDGPGEINVPVSIG
                     GLVVLPGDVVVGDADGVMAIPAGMAELVLGLAEKKEAAEVQSKEKIRNGTNARPWIDQ
                     AIARLNGQG"
     gene            27577..28572
                     /locus_tag="VGC69_09865"
     CDS             27577..28572
                     /locus_tag="VGC69_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295624.1"
                     /translation="MTKDKHTLFRMARSSLLGAGLALVAALPAHADDAYPNKPIHIVV
                     PYTPGGAVDIVTRLVTPKMGEILKQSIIVDNRPGAATNIGMDYVTRQPADGYTLLTAS
                     PSLSSNGALFTNLHFDPAKAFAPVGEIGYAPLVVVVPAASPYKTLKDLMDDGRAHPEK
                     LSFGTAGNGSSGHLAGELLKAEGKFNATHIPYKGGSPAITDLLGGRLAFMPINPLEVI
                     SHLKSGSLRALAVLDAKPAALLPDVPTAAQQGVPGAQAAVWWGLEGPAGMPQAVVDKL
                     NAALQAALADPMVQSRMADLGATLTPGSAADFGTFVANETVKWTKVIKAADIKAD"
     gene            28652..29473
                     /locus_tag="VGC69_09870"
     CDS             28652..29473
                     /locus_tag="VGC69_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295625.1"
                     /translation="MPTRLLKTPYRYFAHLLSLACLLTAGLASASPADDAARQALAPS
                     GVLRVGLYRGSPTSIIENAASHESRGVAYDLGRQLAAALGVPCETVVYPANAPLLKAL
                     ASSEVDVVFTNATAERARHMDFSPVFMSVEKSLLVPGGSSLKTLQDAKRPGLRVGVSQ
                     GSSTSEELLALYPGMSTQPIDTLQHAAQMLASGQIDAFGTNDAILYQMSDGVPGSRVL
                     PGHWGTETFAAGIPKGRQAGMPFLRGFMAAAEADGSVAHAVARADLRGVLPGSAH"
     gene            29532..30581
                     /locus_tag="VGC69_09875"
     CDS             29532..30581
                     /locus_tag="VGC69_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY0295626.1"
                     /translation="MIYTDSLQITPEILGLVAGIDEFKGAWRALGALAPDRLSALRRV
                     ATIESIGSSTRIEGSKLSDREVERLLSNLQIKSFETRDEQEVAGYAEVMELVFSSWQD
                     IALTENHIRQLHRDLLVHSDKDAWHRGGYKTSTNSVAAFDEQGKPLGVVFETATPFDT
                     PRLMAELVAWFNEERGTGRLHPLLLIGIWVVVFLEIHPFQDGNGRLSRVLTTLLLLQT
                     GYAYVPYSSLESVIEQSKEAYYLALRQTQGTIRTAAPNWQPWLAFFLRALSQQVRRLE
                     RKVEREKLVLAKLPDLALQVVEFTREHGRVTMAEAMRLTGGNRNTLKQHFRALVEQGH
                     LVQHGGGRGTWYALR"
     gene            complement(30613..>31240)
                     /locus_tag="VGC69_09880"
     CDS             complement(30613..>31240)
                     /locus_tag="VGC69_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY0295627.1"
                     /translation="PIAGTTRDKVVQEIHIDGVPLHIVDTAGLRETVDTVESLGIART
                     WQEIERADVILHLQDATHPGDALDAGITARLPARTPVLAVYNKVDLLPWPFAAPTGGQ
                     GHAGALGISARSGAGLAELRAELLRLAGWNPGAESPWLARERHLHALQAAAEHLALAA
                     QHASQDDRVLDLFAEELRLAHASLTAITGQFTSDDLLGKIFSSFCIGK"
BASE COUNT         5527 a        10228 c        10013 g         5472 t
ORIGIN      
        1 tgggcctggc ggcgcaactc gaagcgcaga tgcagaccgt ggtcgaccgc tatgaatgcg
       61 aatgggccaa cgcgctgcaa gacccggaaa agctcaagcg tttccgcacc ttcgtcaatg
      121 cgcagggtgg cgacccggac atccggttcg tgcaggagcg cggccagatc cggcccgccg
      181 atcccaggcc ggccagggtc attccgatcg ccgaggagct cctgtaatgc ccggcgccga
      241 aaccccaggc atccagccgg cctggcgcgc ggtttgctcg cgccgcgacc tggtcgccaa
      301 ctccggcgtg gtggcgctgg tcgacggcgt gcaggtggcc ctgttctacc tgccgcaggc
      361 cgagggcccc acgctctatg ccatcgagaa ccgcgatccg aagtcgggcg ccaatgtcat
      421 cggccgcggc atcgtcggcc acctgggcgg ctccctggtg gtggcctcgc cgctctacaa
      481 gcagcacttc aggctggaag acggcgcctg cctggaatat cccgagcagc gcctgcgcac
      541 ctggaaggca cggctcaacg ccgataccgt ggaactcgcc ctttgatttt tttgcgctgg
      601 cgtcttcacc caggcttgga ctctcctacg caaaagggga ttcaccatgc cttaccgcac
      661 tccagcggaa ttcgccgcca acgcggtatg ctgtgcaact ggatggctac gccatgggtc
      721 ttgaacattc ggtcgtgaat atgtacctgt tcccctcagc cctgctcatg ggtagcaagt
      781 tctccatctc cggctgcgcc aagcgcagcc tgggctaggc aggcaaggcc gatcgccatg
      841 gccaggaacc gcatgccggc cacgctgagc ttcctgcttg ccgcgcgccg ctgcgaactg
      901 cacgacatgg aagacctcgc caggacttgc gagctggtca tcctggtcgg cgacctggtg
      961 catgccttgc agaaagaacg gggctattcc aacctgttcc tgcgccatgc gcagccgcaa
     1021 atggccccgg tcctggcagc cttgcaggaa gaagccctgc aggccgagat caagacccgc
     1081 ggcctattgg gcggcctggt gcccgacgag gccctgccca tcatgggcaa gacgcgcctc
     1141 ttcaattgca tcgcctatgc cttgcaccgg ctcgacgagg ccatggacct gcgctggcgc
     1201 gtgcgcgagc gccgcatcga gtcgcaagag gcgaccgcgc agttcacgcg cctgatcgcc
     1261 agcctgcttg ccgtggtgtt cgaggcggcc gacaccgcgc tcgacccgga cgtgacccgc
     1321 acgctggtgg cgctgttcaa tttcatgcag ggcaaggaat tgagcggcca ggagcgcgcg
     1381 ctgggcgtcg tgggctatgc cgcgggctat ttcacggacg cgcagaaggc ccgcatgcgg
     1441 gaactggccg aaagccaggg ccgctgtttc ggcatcttcg ccgaatacgc cccgccggac
     1501 gcgctgcagc gctgggaaca gacgcagtac gacagcgccc ccgtgcagcg catgcgcgcc
     1561 atggcccagc agacttcgcc ggaacaggcc atcgacggcg gcttgagcga gctctggtac
     1621 gacatcgcca cggcgcgcat cgatgccatg cgcgccgtgg cgatgctgct tgcgcaaggc
     1681 ctggcccggc tatgccgcca acgcatcgcc gacacccgcg aagagctcga caaccatcgc
     1741 ctgctcatga gccgttttac ggaccaggcc ggcagcgaga cgcccaccca gctgttcaac
     1801 gtccaggctc gccccatcga cgcgcccttg caggacggac tggccagcgg cacggagcgc
     1861 gccatgctcg acatcatgcg ggagcagcgc ctgcgcatgg accgctacga tgccgacctg
     1921 gcccgggccc gcagcgcgct ggacgagcgc aagaccatcg aacaggccaa gtggctgctg
     1981 gtgcaccatc gcaagctcag cgagcaggcc gcgcacaccc tgttgcaaca gacggccatg
     2041 aaaaacagcc tgtcgctggc ggaagtggcc cggcgcgtcc tgtccgagat cagcgacgcc
     2101 gaccaggcgc aggacggcgc cgatacgccg gccgggcact agggtcagta ctgctcgaag
     2161 cagcgctgcc actgcgcggg atcgcggctc gccgacaggg ccagcatcag cagcacgcgg
     2221 gccttctgcg gattgagttc gccggcggcg acaaacccca ggccggcatc gtcgacctcg
     2281 acgccgcgcg ccaccaggcc gctgccggtg cggctggcgc gcaccacggc cacgccggcc
     2341 gcggccgcgc gcgacaaggc cgccagcgcg gcgtcggcgg cattgccgtc acccacgcca
     2401 gcgagcacga tgcccgccgc atggccggcc agagcatcga tgacgaggcc gtccatgccg
     2461 gcatgggcat ggacgatgtc cacgcgcggc cagccggcgg tttcgagcgc ggccagggtg
     2521 aattcgctgt cgcgggtatg ccgcgtggtg ttccgcgaat agaaatgcgg ctggccgcca
     2581 tgcatgacgc ccgccctgcc ccggttgggc gaggcaaagg cgcagagtcc catgctggcg
     2641 atcttctgga cttcgcgcgc gtagtggatg tcctcgttca tcaccaccag cggacctcgg
     2701 tcgcgcgcgg ccgcgctggc ggccagcgcc acggcgttgt acaggttggc cggcccttcc
     2761 gcccccagcg cggtggcggg acgcatggcg cccaccagca ccagcggttt ttccgtgcgc
     2821 gccaccaggc tcaggaaata cgcggtttct tcgagcgtgt cggtgccatg cgtgatgacg
     2881 atgccgtgca cggcatcgtc gccgtccagc tcgtcgatgc gtcttgccag cgccgcccag
     2941 acggcatggc tcatgttctg gctgccgata ttggcaattt gctcggcctg tatccgggcc
     3001 aggccagcca gggcgggcac ggcgtcgacc aggttcgcaa ccgacagcgc gcccgcctta
     3061 tacgtcgccg tattggcctg ggcctgcgcg cccgcgatcg tgccgcccgt ggcgagcagc
     3121 gcgatccgcg gcagggcccg ggcctggtca ggcatcgagc actccggaca ggccctggcg
     3181 ctcgagcagg gagttcaggt gctcccagcc atggaagtcg atgacgattt gccctttttc
     3241 cttggcgccg atcttgagcg acacgcgcgt gcccagatga tcggacaagg cttcttcgag
     3301 gcgggtgaca tcgcgcgaga cgctcttgcc ctttttagcc gtggcggttt cagcctcctt
     3361 ttgggttcgg gcgacgagtt tttcggcctc gcgcaccgac agccgcttgg ccacgacctg
     3421 gttggccagc tggatttgcg tggcggcatc gaccgacaac agcgaccggg cgtggcccat
     3481 gtcgatatcg ccagccagca gcatggtctg cacggccgcg gccagattga gcaggcgcag
     3541 caggttgctg gtggccgagc gcgatcggcc gatggcctgc gcggcctgct cgtgggtcag
     3601 gccgaattcg tccagcagcc ggcgcacgcc ctgggcctct tccagcggat tgagatcttc
     3661 gcgctggatg ttctcgatga gggccatgac cgcggcgttt tcatcgggaa cgtcgcgcac
     3721 caggaccggc acttccttca ggccggccat ctgggcggcg cggaagcgcc gttcaccggc
     3781 gatgatttcg tattcgcccg gcgtggcctc gcccaggccg cggaccagga tgggctgcat
     3841 gatgccctgg gtgcgtatgg attcggccag ctcggcgagc gcgccttcgt ccatgcgggt
     3901 gcgcggctgg tatttgcccg cgcgcagctt gctgacgggc aataccgacg gcggaccttc
     3961 cttctggctg gcggccttgc ccagattgtc gacggcaggc gcatcggcgc ccagcaatac
     4021 atccaggccc cggcccaggc ccttgggttt tttcgtcgcc atgcgattcc tctttctcga
     4081 tttccgtttt tgacggcttg tttattgctg ggcgccggcc agcgaccggt accagtattg
     4141 gtggtgttcg gtaaagccat agcgggcgta gagcgtccgc gcggcctgat tgccggcctc
     4201 gacctgcaaa taggcctgcc gggcgccctg ccgcaggccc tcttgcaact gcgcctgcag
     4261 cagctgagcg gctatgcctt gccggcgccg cgcggcatcg ctgatgacat cgaaaatgcc
     4321 catcaggccg ccatcgaaca cgcccaacgc ggctccgacg cagtccgtgc cgtcataggc
     4381 cagcagcgcc tgcttgggca tggccacgct ggcaagccgc aaggcatgct cggcaatata
     4441 ttccgggctg gatcccttga gccgtccgac ttgcgccgcg aacgtccgcg tgtccgcgac
     4501 gcgcaattgc aaggccccct gcccttgccg ggcggcggcg tcggccgcgg cctgcatgga
     4561 cacggccatg accagcgtgt tgccataggc cttgaagccg cgcgcatgca gggcgggctc
     4621 cagcgcggcg tcgggacagg catcggtgaa ccggaaaagc aggggcaggc cgtgccgcgc
     4681 atagatgccg ttcacatacg ccaggcgttc gtccagcgcc accggggacg gcgacaggat
     4741 gctgacgctg cgcgcgcgct tggccgagga ctgcgcccag cggaccagcc agccgtgata
     4801 gagctgctgc tcgggcacga ccgtggcgtt cagcgaggct tcttcgatgc gggtcacatc
     4861 gaccgcgccg ggcgtcttgc gattttcctg catggtcagc tctcgacctt catggccttg
     4921 acgcgctcga tcatctcgag gccgaaagca atataggcct gcgcgccgcg cgaactgcgg
     4981 tcgtagacca cgccgggcat gccgtagctg ggcgcttcgg ccaggcgcac attgcgcggc
     5041 acgaccgtct tgaagacctt gtcgccgaag tgcgactcga gctgcgcgga cacttgctgc
     5101 tgcaaggtca tgcgcggatc gaacatcacg cgcagcaagc cgatgagctg cagttcgccg
     5161 ttgatattgc gatgcacgcg cttgatggtg ttgacgagat cggacaggcc ttcgagcgcg
     5221 aagtattcgc actgcatggg gatgatcacg ccatgcgccg ccaccaagcc attcagggtc
     5281 aggagcgaca aggtgggcgg gcagtcgata agaatgaaat cgtactggtc ggcgacgctg
     5341 ctgatggcct gcttgagctg gcgttcgcgc tcttccattc cgaccaggtc gatttcggcg
     5401 ccggccaggt cgcgattggc cggcagcaca tcgtagccgc ccacttcgga gcgcacgcgc
     5461 gcatcggtga tggtcgcctc gtggatcagc acctggtaga gcgtggcatt gagcgtgctc
     5521 ttgtcgatgc cgctgcccat ggtggcattg ccttgcgggt cgaggtcgac cagcagcacg
     5581 cgctgcccct gcgtggccag gcctgcagcc aggttgatgg ctgtcgtcgt cttgccaacg
     5641 ccacccttct ggttggcgat gcaaaaaacg agtgcggacg gcttgctctg ggacgcggag
     5701 gcagtcatgg ggttcctgtc ggggttgctg cgtaaagctg aattgcgttc gaggtttcag
     5761 gcctgtccgg tgcggcgcat ccagaccagg cagcgttgcg catcgagttc aggcacgtgc
     5821 agcgtttcaa tgcgccgcac ccgccagacg ccttgcgcat gcagcgcggc gatttcgtct
     5881 tcgggggttt tgcctttcat ggcaagcaag ctgcctttct catgtacatg caagcctgcc
     5941 aagttagcaa agtccagcaa tgatgcaaag gcacgcgaaa ccacgacatc gcagccggct
     6001 tgcggcaagg tctcgacacg cgcatggctg gcctgcagat tgggcagggc cagcgcgccg
     6061 cttatctggc gcacaaaggc cattttcttg tccacggcgt cgacgcactg gacccgccat
     6121 cgatcgtgca ggatggccag cacgacgccg ggcagtcctc cgccggatcc cacgtccatg
     6181 atcctgagcg gccgcggcgc cgtttcgttg tccgctgttt cgttttcgat gggccgcgcc
     6241 acggccaggc tgtcgaacac gtgctgcacc agcatctggc cggcgtcgcg cacggccgtg
     6301 agattgtagg tgcggttcca gcgctgcatc tgctcgacat agcgcagcag tttgtcgcgc
     6361 tgcgcgtccg aagcccgcag tccgagctgc tggcaagctg ccgtcagccg ccgcgcaaaa
     6421 tcggcggaag tcgctacggc ctgattcatg ccgcctgctt gcgcgcgccg taatgcaggc
     6481 gcttaagatg aatgagcagc agagaaatgg ccgcgggcgt gacgccggaa atgcggctgg
     6541 cctgccctat ggtctcgggc ttgtgcgcct tgagtttctg gcggacctcg aaggacaggc
     6601 tggtgaccgc gtcgtagtcg atatcggcgg ggatggactg ctgctcatgc tcgacgtgct
     6661 tgcgcacctc gtcctgctgg cgcgtgatgt agccggcgta tttggtctga atctcgatct
     6721 gttcggccaa cacctcgtcg tcgctcaggc ccggacctgc cagcaggctg ccgtcgccca
     6781 gcctggccgc catgagcttg gcgtagctga cgttgggacg cttcagcaga tctgatagtg
     6841 agtactcacg ctctatcgct ttgcccagca aaggctccgc cgtctcggcc ggcatcaggc
     6901 gtggattgac ccaggtggat ttgaggcgtt ccagttcttg ggcaacggcg tcacgcttgc
     6961 ggctgaatgc atcccagcgg gcatcatcga ccaggcccag cttgcggccg atttcggtca
     7021 ggcgcaggtc ggcattgtct tcacgcaagc tcaagcggta ttcggcgcgc gaagtgaaca
     7081 tgcgataggg ctcggtgaca ccccgcgtga ccaggtcgtc gaccagcacg cccaagtagg
     7141 cttcatcgcg acggggcgtc caggcttcct tgccttgcgc ctgcagcgcg gcattcaccc
     7201 cggccaacaa gccttgggcc gcggcttctt catagccggt ggtgccgttg atctgaccgg
     7261 cgaaaaacag accggagatg gccttggttt ccagcgtgga cttgagttca cgtggatcga
     7321 agtaatcgta ttcgatggca tagcccgggc gcaggatatg cgcctgctcc aggccgggca
     7381 aggaatggat cagatccagc tgcacatcga aaggcaggct ggtggaaaca ccattgggat
     7441 aaacctcatg cgtgtccagc ccttccggct cgaggaagac ctggtgcgag tccttgtcgg
     7501 caaagcgatg gatcttgtct tcgatggacg ggcaataccg cggacccacg ccttcgatca
     7561 cgccgctata catgggcgag cgatccagcc cgccgcggat gatatcgtgc gtgcggctgt
     7621 tggtatgggt gatccagcaa ggcagctgcc ggggatgcat ggccgcgttg cccatgaaag
     7681 aaaacacggg gatgggattc aggtcgccgg gctgttcttc aagcgcgctg tagtcgatgg
     7741 tccgaccatc gatgcgcggc ggagtccctg tcttcaagcg tccttgcggc agcttgagtt
     7801 ctttcaaccg ggcacccagc gaaatggccg gcggatcgcc tgcccggccg cccgagtagt
     7861 tttgcaaccc gacgtggatc aagccattga ggaaagtccc tgccgtcagc accacggtac
     7921 gggcccggaa cttgagtcca atctgggtga cggcgccgac aaccttgtcg ccctcgacca
     7981 tcaggtcatc gacggcttgc tggaacaacc aaagattggc ctgattttcc aggcggccac
     8041 gaatcgcctt gcggtatagc acccggtctg cctgggcgcg agtagcacgc acagccggac
     8101 ccttggaact attaagaatg cgaaactgga ttcccgcttc atcggtggcg atggccatgg
     8161 cgccacccag ggcgtccacc tctttcacca gatgcccttt gccaatgccg cctatcgagg
     8221 ggttgcagga catctgccca agcgtctcga tattgtgcgt gagcaggagc gttgttgcgc
     8281 cagtgcgcgc cgaagccaga gcggcttcgg tacctgcgtg accgccacca acgacgatca
     8341 catcaaattc gcgtgggaaa tccatagtga taaagagaaa gagcgttcgg gcttcattat
     8401 agagtctggc gcattggtgt ttcacgtgaa acatcgaaat tgctggtaag cccactgttt
     8461 cacgtgaaac catggccaca gcttgtcggc gagcccgcta tccgacagag ctatccacac
     8521 cctaccccgg attgcaatat gaaggtcatc aaaggattcg ggcgcataaa gagagatggg
     8581 gatatctttg tgaataaata gccagaatca acctacaggc cctacccatt tcaaggaaaa
     8641 ctccctttta cccagtaagc cagcagcggg aacgatcggt ccgacttcct ggctatcatg
     8701 tgaacaaccg gtgggcttcg ggccttgcac gagcaataat caagaaagtc cacgcgcttc
     8761 gacggccagt tatcccaact tatccaaccc ttatcccggc cttatccaca tgtttttccc
     8821 aagcctgaaa atgacaacgc cccaaaggct tgggccttgg ggcgttgtgg ccagacgacg
     8881 ctggcaagca ggaaacggga tttcccgcga tcagagcggc ttgatctcgg ccgcctgggg
     8941 tcctttcgga ccatccttga cttcgaattc gacttcctgg ccttcgttca ggctgcgata
     9001 gccacgaccc tggatggccg agaaatgtgc gaacacatcc gtaccaccgc tgtcaggggt
     9061 aatgaagcca tagcccttgt cagcgttgaa ccacttgact ttgccttttg ccattttcta
     9121 gagcgtcctt gattgataaa acgagatgca aatgaatcaa ggacgccaaa accaatatgt
     9181 ctacgcaaac cggacttgcg cactgcttga atcaactgca taaggcagca tactcagccg
     9241 ttacccaagg aacactagat attttcccta ggttattttc catatatcca ttgggcgcat
     9301 tcaaataact taatcgcgca acccacgtct tattatcacg actgattaac ccgatcgcca
     9361 ataacaaaac ctcatttgaa ttccatgctg caatctaaac ttcccgatgt cggtacgacc
     9421 attttcacgg tgatgagccg gctcgcccag gaacaccagg ccatcaacct gggccagggc
     9481 tttcccgatt tcaacccaga ccccgtcctg tgccagctgg tcgcgaaggc catggctgaa
     9541 ggccataacc agtatcccta catgaccggc gtgccgcccc tgcggcacgc cattgcacgc
     9601 aaggtccagg ccctctacgg ccgggcctat gaccccgata ccgagatcac ggtaaccagt
     9661 ggcgcaaccg aagccctgat ggccactgtg ctggctgtgg ttggaaccgg cgatgaagtc
     9721 gtggtgatcg agccctgcta cgactcctat ttgccagcca tccgcctggc aggcggtatt
     9781 ccagtccctg tgcccatgca ggctcctacc cgggaggatc cctgcttccg ggtggattgg
     9841 cagcgtgtgc gcgatgccat gacgcctcgc acccggctga tcatgatgaa ctttccccat
     9901 aatcccacgg gaatcgtcct gcaggaacag gacctggatg cgctggaaag catcgttcgc
     9961 gacaccggcg tgctgctatt gtccgacgag ccctacgaac acatcatctt cgacggtcaa
    10021 ccgcatcgca gcctggctac ccgtcccctg ctggcagacc atgccttcgt tatttcctcg
    10081 ttcggcaaga cctatcacgc tacgggctgg aaagtcggct attgctgcgc acccaaggca
    10141 ctgaccgccg aactgcgcaa aatccaccag ttcatcgtct ttaccgtgtc ttcgcccatg
    10201 cagttcgcct tggccgaata catgcaagac cccaagccct acctggaact gcctgccttc
    10261 taccaggcca agcgcgaccg cctggctacg ggtctggccg acagccgttt caaggttctg
    10321 cccaaccctg gcacgttctt cctgctggcg aactacagtg acatttcccg acagagcgag
    10381 gcggatttct ctcgctggtt gaccgtcgaa catggcgtga cggtcattcc ggtttcggcc
    10441 ttctaccggg atccggatgc gccgcaatcg aatcaccagc tggtgcgctt ctgcttcgcc
    10501 aagcgcgaaa gcacgctgga tgccgcgata gaaaaactgc agaaggtctg aaccggcaaa
    10561 ttcctgccct tctggcacca cagacccgcc tgcaggcggg ttttttgttt ccagccaagg
    10621 agccagacgg gattattgag acagctgggg caaggacctc aggccctgcc gaacggaaag
    10681 tcgacgtcga ggcaggactg tagcaccaat caggcaatcc tggtgcaggc aggttttgct
    10741 ggttttcttg ccgtggctgc gcgagcctgg cgtgtcttat cttttatcca atgctttttt
    10801 ctatatctac ttggtggtgg tggatgagtg tctgtggata actgcatcaa ccaaaaaaat
    10861 tacatatatt tcaatgggtt gtggggtttt ttgcttgtgc agggaaaggc ttttgagtgt
    10921 ctggcaaaga tgaacaacat ttcggccaag ctgaaaaagg gctagttgtt catcttgggc
    10981 ccacagactg tccacacatc ccggtctcag cgcttatccc cggttatcca caagctggaa
    11041 tagtgtggcc acgcgataaa aacctggatt cggatgccat gaaatgaaaa aaatcatgta
    11101 accgtggcga gtcaggatcg acagcaggcc gtgatcctga catgctgacg gattgccccg
    11161 ccggggctgt ttctcttttt ttgccatgaa actcgatttg ctggtgctcg ttcccctgga
    11221 cgacgagaac aaggcttccc ttgccaatca cttcgaaatt catcatgtcc ctgataaaaa
    11281 ggaccaggcg ccgcaacaac tggcgcaaca tgcccaagtg cgggcaatac tgacgaacgg
    11341 cacggtggga tgcactgccg cacaactggc ggcgctgccc aaggtggagc tggtctgcgc
    11401 tctcggtgcg ggttacgaga acatcgatct cgaggcgatg cggcagcgcg gcattaccgt
    11461 cggcaacggc gcgggcacca acgacaactg tgtggccgac catgccatgg gcctgttgtt
    11521 ggccgccgtg cgccacattg tccaactcga tccctacacg cgagccggcg gctggcgcga
    11581 tgccctgggg cccctgtcca atgtatcggg caagcgcatg ggcatcctgg gcatggggat
    11641 catcggtcgc cagatcgcca agcgcggcct gggtttcgac ctggaaatcg gctatcacaa
    11701 ccgccgccag atcgaaggcg agccctatgc gtatttcggc agcgccaagg aactggcggc
    11761 ctgggcggat tatctgatcg tggtgacccc gggcggcccc actaccaggc acctggtcaa
    11821 tgccgaggtg ctcgaagccc tgggtccgcg gggctttgtg gtcaacgtgg cgcgcggcag
    11881 cgtggtggat acggccgccc tggcggcggc gctgaagggc ggcgtcatcg ccggcgccgg
    11941 actggatgtc tacgaaagcg aacccaagcc gccgcaagcc ctgctggagc tggagaatgt
    12001 cgtgctgacg cctcacgtgg cgggcaattc accggaagcg atagcggcct cgtaccagcg
    12061 cttcatcgac aatgcggcac ggtacttcgc cgggcagccg ctgatctcgc cggtcccgct
    12121 ctaggcaggt cctgcccgaa aaaaacagcc cggcggattt gctccgccgg gctggaaaat
    12181 tcacggcgcg gcggcaagcg ccgcgccgtg ggacaaaaca gcaggtcagt cgatcttggc
    12241 gccggatatc ttcacggcgt cgccccactt cttgatttcg tcgaccagga agtcgtggaa
    12301 agcctcgggc gtggaaggca cgaactggct gcccacggtg aggatcttct tctgcgcttc
    12361 gggatcgctc atcacttcgc gcatatcggc cgagatcttg gccgtcagtt cggggctcag
    12421 gcccttgggc gcgaaaaaac ccgaccaggg tgcggcggca tagcccggaa agccctgttc
    12481 ggccacggtg ggatagtcca agccggggaa gcgatcggcg gtggtgatgg cgatcaggcg
    12541 cagcttgccg ctctgcgcgc ccggcaggat ggccggcagg ccggtgatgc ccacctggat
    12601 ctggccggcc atcaggtcgg tcagcatggg agcgctgccc ttgtagggca cgtgcgtgat
    12661 cggaaattgg ccgcgctgct tgaggatttc caggcccagg tgcgaagcgg tgccgttgcc
    12721 atccgagcca taggaatact tgttgccgtc tttcttgagc agcgcgatca gttcctgcag
    12781 gttcttggcc ggcaccgacg ggttcacggc gatggcgttg ggatacaggg tggccatggt
    12841 caggggcgtg aagtccttga tcgggtcgta gggcaggttc ggatagagcg agacattgat
    12901 gccgtgcgaa cccgtcacgc ccagcagcag cgtgtagcca tcgggcttgg tgtgggccac
    12961 gtattcggaa ccgacgttgc cgccggcgcc gggcttgttg actacgatga ccgattggtt
    13021 ccacttcttg ctcaggcctt cggccacgat gcgcgcgctg atgtcggttg ccccgcccgg
    13081 cgggaaaggc accacgatgg tcacgggatg ggaaggatag gcatcctcgg ccatggccat
    13141 gccgggcagc gcggcggcaa ggcctagcgc cagcagggct ttgccgacgt ggcggcgaga
    13201 cggacgggtg agggaggaaa caacgtcgga aatcattaat tggcctttca taaatttatt
    13261 gataaacaac taaagagcag gcgtgatgcc ttggcttgtt cttattcgat cgtggtcagg
    13321 cgcttttcga gggcgcgcat ggcttcgtag cccatcagat tcatgatgtc ttcgataccc
    13381 acggccacgt cgggacggtg cacgggttcg ccagtggcga tgacttcctt catgacttcc
    13441 agcgcctgct gcatggcgtg gatggccgag gccggcaaca tgcgcggcag gctgatgcgg
    13501 cgcacgccga gttccttcag gcgcggcagc ggaatcagcg gcgtggtggg acgcttgcgg
    13561 atgccgaagc ccatattgat ggaaaccgga atgccggcgg cgtcgacgat gcgcttgatt
    13621 tcgtcctcgc tgtgcacggc atcgggaaac agcatgtcgg cgccggccgc cgcataagcc
    13681 ttggcacgct tgatggcgcc ttcgatgcct tcgatggcca gcgagtcggt gcgggcgata
    13741 atgacgaagt cgtcgtcgcg gcgggcttcg caggcggcct cgatcttctt gaccgcttcc
    13801 ttgatgtcga tgacgtcctt gccgggcatg tggccgcagc gcttgggcga gacctggtct
    13861 tcgaggttga tgccggccac gccggcttct tcgaacatgc gcacggtgtg gaacacattg
    13921 accgggttgc catagccgtt gtcggcatcg gccgtcagcg ggatggacac gctgcgggcc
    13981 aggttgcggc agtggttgac gttttcggtc aggcccatca cgccgatgtc ggggatgccc
    14041 agcagcgcgt tggaaatggc ggcgccgctg gtgcaggccg tcttgaagcc ggcggcctcg
    14101 accaggcgga tgctgtagcc gtcatagacg ccgggggaga cgatgaaatc acccgacttg
    14161 atcagggcgc ggaattgact ggtggccttc atggggttct cctgaatatt cgaattcgat
    14221 agggcttttg gaaagagcgg cgcgtcacct gctcagcgac gaggtgctca attgttttgc
    14281 aaggttgtcg gtggcccggc cgatgtgcga gcgcatcagc gattcggcca gcgcggcatt
    14341 gcgggctttc atggcgtgca ggatttgcca gtgttcttcg taggcgtctt ccttgcgctc
    14401 gatgacgacg ctggtcgaat gccggcggta gatgcgcagc aggtggtaca ggtcgccgca
    14461 cagcgcgttg atgatgcggg tgttgccgca ggcgcggacg atgcgctcgt ggaagtcgaa
    14521 caccttgggc gtggggtcgg acctggggtg gtgatggctt tcgagctcgt gcagtagttg
    14581 ctcgatgtcg acatcgttca tgcgccgggt ggccaggctg caggccatgc cctcgagcgc
    14641 catgcgcagc tcgaacaatt cgatggccga ttcggtggac agcgaaatca cccgcgattt
    14701 ggcgtagggt tgccgggtga tcagctggat gccctgcagg cggcgcacgg cttcgcgcac
    14761 cgggccgcgg ctgatgccga actcttgcgc cagggcgagt tcgttgacca cgctgccggg
    14821 aggaagctgg ccttggtaaa tcgcgtcgag cagatgttga taaaccacgt ccgccagggg
    14881 ggcgtcgttg tcgtccaaat cggccttggt gctttcttgg atatccatag tgttaacaat
    14941 ttaatggtgt ttcagctaaa tggtcaataa atcaacggga aaattacgga taattagggg
    15001 ttttccctag acagtgttga cactttgaca aataaaaccc tacattcatc aatatcaacg
    15061 aactcagacc agccgctttt ccatgcaacg caccctcgat tgccccgccg gcgcctatca
    15121 atatgtggac attagcgatg tttcccgccg gttcggtcgc gcggtcgaaa ccctgcctta
    15181 cgtccatcgc gtgttgctgg aaaacctggc gcgcagccag gcgtggggcc tgcctgtcgc
    15241 cgagaacgag atccgcgcgc tgctggcgca agaccagggc gccgacctgc ctttgtatgt
    15301 aggccgggtc attctcccag actccagcgg cctgcccgtg ctgcaggacc tggcggccct
    15361 gcgcgacgcc gtggcgcgcg ccggcggcga tgccagcgcc gtcgaggccc gcattcccgt
    15421 cgacctgatt gtggatcatt ccctgcaggt cgaccactgg ggcgacagcc aggccgtcat
    15481 gctgaacctg ggccgcgagt tccagcgcaa cggcgagcgc taccgcttcc tcaagtgggc
    15541 gcagcaggcc tttcgccgcg tccatgtctt tccgcccggc acgggcatca ttcaccaggt
    15601 caacctggaa cgcatcgcca cggtggtcag cgcgcgtgag cagaacggcg cgcaatgggc
    15661 cttcccggac ttcgtcatcg gcggcgactc acatacgccg acggtcaatg ccctgggcgt
    15721 gctggcttgg ggcgtgggcg gcatcgatgc cgaagcggcc atgctgggcc aggcctatac
    15781 cttcccccgt ccgaacgtgg tgggcgtgcg cctggtgggc agcatcgtgc cgccggcgct
    15841 caccaccgat gccgcgctcc tgatcaccca gcgcctgcgc gaaatcggcg tggcgggcga
    15901 catggtcgaa tacttcggcc ccgccgtggc gggcctgacg gtgcaggaac gcgccaccat
    15961 cgccaatatg tcgcccgaat acggcgccac ctgcggcttc tttgccgtgg acgagcagac
    16021 gctgcgctat ctgcgcgact ccggccgcag cgacgcgcaa gtcgagctgg tcaaggccta
    16081 ttgccaggcc aaccacttct ggcgcggcga agatgcgccg ctgcccgagt acgaccgcgt
    16141 cgtggaaatc gatttgagcg aagccgcgcc cagcatggcc ggcccgcgcc gcccgcagga
    16201 ccgcctggtg ctgggcgcca tctccggcga cttccgcggc cgcctggcca agcccttgtc
    16261 cgaaggcggc ttcggcgcca agccgctggc cgggacgaaa gatcggctca agcatggcga
    16321 catcgtgctg gcggccatca cgtcctgcac caacacgtcc aatccggtgg tcatgctggc
    16381 cgccggcctg gtggctcgca aggccatgga acatggcctg cgcgtgccgt cgtgggtgaa
    16441 gacctcgatg gcgccgggtt cgcgcgtggt cacgcagtac ctgcagcgcg ccggcctgct
    16501 ggcgcccctg gaaacgctgg gctttgccgt gatcggctac ggctgtacca cctgcggcgg
    16561 caagtcgggt ccgctcgatc ccggcgtggc cgagcagatc acccggaacg acctggtcgt
    16621 ggcctcggtg ctgtcgggca accgcaattt cgaaggccgc atccacaagc tgatccgcgc
    16681 caactacatc ggcgcgccgc ccatggtggt gatctatgcg ctggccggcc ggatcgacat
    16741 cgacttcgcc gccgagccgg tggggcacga cgccgcgggc aagccggtct tcttgcgcga
    16801 cctgtggcct actcccgacg agatcgcgca gctgctgccg ctggccaacg accacgacct
    16861 cttcaacgag atctacgacg gcgaaagcga cggcgcgggt ccgtggacgg cgctcgaagc
    16921 gccgcagggc gagcgctttg cctgggatcc gaaatccagc tatctggtcg agccgccgtt
    16981 cttccacgac gcctcgggcg gcaacgccat cgccaagttg gccgacagcc tcaagcaggc
    17041 ccgcgtgctg gcggccttcg gcgactcgct gaccaccgac cacatctcgc ccagcggcga
    17101 aattcccgcc gagacgccgg ccgggcagta cctgcaatcg ctgggcgtcg cgcccaagga
    17161 tttcaatacc ttcgtgggcc ggcgcggcaa cttcgaagtg atgacgcgcg gcaccttcgc
    17221 caacatccgc atcaagaacg tcctgctgcc gcaggaagaa ggcggctata cgcgccattt
    17281 tcccgacgac gtgcgcatga cgattttcga cgcggcggcc gcataccgcc aggccggcgt
    17341 ggccagcatc atcctgggcg gccgcgaata cggcaccggc agcagccgcg actgggccgc
    17401 caagggttcg gcgctcctgg gcgtgcgcgc ggtcattgcc gaatcgtacg aacgcatcca
    17461 tcgcgccaac ctggtgggca tgggcgtgct gccgctggcc ttcatgcccg gccagggctg
    17521 gcgccaattg ggcctggacg gcagcgagag cttttgcttc gagcgcgtcg aagacgccgt
    17581 gcgctcgggc ggcacggtgc tggtgatcgc gcagggcaag tcgggcgaga tccgcttcga
    17641 agcccagccc caggtgctga ccgaggccga gcgcgggctg atggccgccg gcggcatccc
    17701 ggcccatgtg ctgcagtctt tcctgtcgcg ggccgagggg cccgccgcgg cgtaatcatt
    17761 tttataggca acgtttccat gtctctcgat ctgcttctga ccaatgcgcg gctgatgttg
    17821 ccgcaacgcg gcctgacccc tggcgtggtc ggcgtgaagg acggccgcat cgccgtcatc
    17881 gccgacaagg cttctggcct gtcggcgcaa gacaccatcg attgcggcgg cctgtgggtg
    17941 ctgcccggcg tgatcgatcc gcacgtgcat ttcggcttcg gatcgcccga gaccgacttc
    18001 gccaccgaat ccaagaccgc ggccatcggc ggcacgacca cggtgctgag cttccaccgc
    18061 tcgaaggaca tccgcgaaag cgccgacgcc gcgcgcgacc tgggcgagag ccagagctgc
    18121 atcgacttcg gcttccactt cggcattacc agccatctgc acgtgcagac gctggaagaa
    18181 atctcgcgcc gcttcggcgt gtcgtcgtac aagctgtaca tgatgtacaa gggtgcggcg
    18241 ggcctgtcca agggctttac cgacatcgac gatggcctgc tctacgcggc cttgcagcaa
    18301 gtggccgcca tccccggcgc cgtgctgggc gtgcactgcg agaacgtcga ggtgatcccc
    18361 gtgctgcgcg agccgctgcg cgccgcgggc cgcgacgatc tcaaggcctg gaacgaacag
    18421 agccccgact tcctggaagc cgagaacgtg caccgcgtgt gctatttcgc ctcgcgcacc
    18481 aggacgccgg tgaacatcgt gcacctgtcg tgcaaggaag cgctcgacga agcccgccgc
    18541 catcgcctca agaaggccgc gcccatctac gtggaaggct gcccgcacta tctcttcctg
    18601 aacgacgaat cgcccgccgg cacctatgcc aaggtcaacc cgccggtgcg cagccaggcc
    18661 gacgtggagg ccatgtggga aggcgtgatc gacggttcca tcaccaccct gggctcggac
    18721 catgtgccgc gcaagcgcgt caccaaggac aaggacatct ggacggccag caacggcttt
    18781 cccggcaccg gcatgatgct gcccatcatg ctgcacgagg gctaccacaa gcgcggcgtg
    18841 ccgctggaaa agatcgtcga gctgttgtcc tacaactcgt cccgcctcta caacatgccc
    18901 gccaagggca gcatcagcgt gggctgcgac gccgacctgc tgatcgtcga ccccgatctg
    18961 caacgcaccg tcgaccccgc cgaactggaa tcgaatgccg attattcgcc gtacgaaggc
    19021 atgaccctga agggttggcc ggtacgcacc ctggtgcgcg gccgcaccat catgcaggac
    19081 ggccgcatta ccgatgacgc ccgccggcag cccggcggcc gcttcatccg ccgcctttga
    19141 ttcgacgcat caacgcggca aacacgaccc ctaccgatca tcgcaaagag accatcatga
    19201 gcgaaaccaa gaccaaacgc atttgggacg acttcctgac cgagcgcgac caggaagtcc
    19261 tgaaattcgc cggctacggc aagcgcggcg gcttcggcaa acgccccgcc ctgttcatca
    19321 tcgacgtgca gtacaacttc acgggcgaca cgcccgagga catcctcgac ggcctgaaga
    19381 cctaccgcac gcactgcggc cgcgaagcct gggccgccgt cgagcacatc atccccctgc
    19441 tcgagctggc ccgcgaaaag aacatgcccg ttttctacac cgaaagcggc cgccgtcccg
    19501 acctgatcga cagcggcgtg caagtgggca agaaccaccg cggcggcgaa aagacggtga
    19561 tcgccggcac gcacgccacg cagacggtgg agccgctggc gccgcgcccg caggacttcc
    19621 tcatcagcaa gcagaagccc tcgtgcttct tcggcaccac cttcatgagc cagctgaatt
    19681 tcctggacgt ggacacgctg atcatggtgg gctgcacctc gtcgggctgc ctgcgcgcca
    19741 ccacggtgga cgcctactcg tacaacttca agtgcatcat ccccgaagag gccgccttcg
    19801 accgcttcca ggccagccat gccgtgaacc tgttcgacct gaactgcaag tacgcggacg
    19861 tgatccccag catcgaagtc gtcgaatacc tgcgcaacca gccgcagttc gtccgtccct
    19921 aagcctcggg cgatccggca cgcatcatgt cccaggcccc gccgctaccg gtctacttgc
    19981 tcacgggctt tctgggcagc ggcaagacca cgctgctggc ccggctgatc catcagccgg
    20041 ccttcgcgga cacggccgtc atcatcaacg agttcggcga tgtgggcctg gaccatgtgc
    20101 tgctggagaa atccgacgac cgcgacgtgg tgctgctgga gtcgggctgc ctgtgctgcg
    20161 ccagcaacag ccccttgcag gacagcctcg aagacttgta ttaccgccgg ctgcgcggcg
    20221 acatcccggc gttttcgcgg gtggtggtgg agacgtccgg cctggccgat cccctgcccc
    20281 tgatgaacgc catcaacgcc tcggccacgg tgtcgcgcca gtaccgcttt gccggcgtcg
    20341 tcacggttgt cgatgcggtc aacggcgcgg ccagcctgcg cgagtaccgc gagtgcgcgg
    20401 tgcagctcgc cgtggccgac cgcgtggtgc tgaccaagac cgacgtggcc tcggccgccg
    20461 agaccgaagc cgtgcgcggc ctgatcgcgc aggccaaccc gcaggccgat gtgcttccgg
    20521 tcacgctcga cagcgagatc gacctgctgc gcgggctgga aatgcgcgag cacgcgccgg
    20581 tggcgccggt ggcgggccag gcggcgggca agttctcgca tctgctgcgc tacggcatca
    20641 ccagccacgt gctgcgcgtg aacggaccgg tgggctggcc gcgctatgcc gcctgggtgg
    20701 ggactgcgca gcgcgtgctg ggcgagcgcc tcctgcgcgc caagggcatc ctggagttcg
    20761 aggatggcga ggtctgcgcc atccatggcg tgcgccatct gttcggcacg cccaggccct
    20821 tgtccggccc ggtgccggcg ccgcaatgcg gcgccgtggt gctgatcacc agcgacgtgg
    20881 atgaagaaga tatcgagacg gtcgcccgcg cgctgggcgc gacggtgatg ccccaggcgg
    20941 tcttggctgc catgtaagaa cttccttgaa aatgggattt gaccatgagc aatacggatg
    21001 tgatcgtcgc cggcgcgggc ccggtcggcc tggtggccgc agtggtcctg gcgcaagccg
    21061 gtttgaaggt ggccctgctg gagaagcgcg atcagctgaa ccaggcctcc aaggcgtcga
    21121 cctttcaccc gcccacgctg gccatcatcg atcgcctggg cgtgttcggc atcatggaaa
    21181 gccatgcgca gcacgtgggc tcgcagcagt accgcacgac tgagcgcggc gtgatcggca
    21241 atttgtcgta cgaccagctc cagggcctga ccacgcatcc tttccgcaag cacctcgagc
    21301 agtcgcgcct gacgccgctg ttgctggaaa aattcaagac cttccccggc gcgcaagccc
    21361 atttcaacac cgagctggtc ggcatcgacg ccaatgacga cagcggcgtg acggcgaccg
    21421 tgctgcagga cggccagcaa aagcagctca aggcccgcta cctgctgggc accgacggcg
    21481 cgcacagcaa tgtgcgcgaa gccgcgggca tcgccttcga cgccaggccc tacccgggcc
    21541 gcgtgctgcg cgcccgcgcc gaaccggcga gcctggatcg gatcctgccc ggcatggcgg
    21601 ccatcaccta cctggtcaat gactcgcact cggccagctt cctgcgcatg cccgactgct
    21661 ggcgcatcat cctgcgcgtg cccgccggca ccagcgacga gaccgcgatg gccgatgact
    21721 ggatcttcga gcgcctgcac gccctggcgc ccgccatgcg cgccctgccc acgctggtcg
    21781 gccgcgacgt gtacggcgcc agcaaggcca tggccgcgca gaaccgcgcc ggcaacgtct
    21841 acctctgcgg cgacgcggcg cacctgacca acacgcgcgg cggcatgaac atgaactgcg
    21901 gcattcatga cagctacacc ctggcgcgcg ccatggtgcg cgccatacag gacggcgacg
    21961 cggccggcct gcacgccgcg gcggctgagc gcctgcgcgt ggtgtccgag gtgctgctgc
    22021 cgcgcaccga ttccatggtg tcgcaggaag gcacgtggat cgaacgcgtg agcaagctgc
    22081 tcaacgagcc cgaccacgcc cgcgattacc tgtacaagtc cgcgatgctg gacatggtcg
    22141 atctgtcggt ctgatcccgc tgcaacgcag ccacgcataa cgagactcct catgaagaat
    22201 gcattgccca tgctgcccag ccccgaatac cgcctgggcg tgctggtccc gccggccaac
    22261 cccacggtgg aaatcgaata cccggcgctc gtgcccgcgg gcgtggcgct gcacgccatg
    22321 cgcttgccgg tgattcccgg cgacctggat gcgcgcaacc gcggctacgt gccggcctac
    22381 gccgaatgca tcaagggctt cggctcgctc aagctcgatg ccattgccgt ggccatgacc
    22441 ggcccgcagt accgcctgct gttcgacggc gacatcgacc tgtgcaagcg cctgtcggac
    22501 gccgccggca ttccggtgga aaccgccagc atggcgctct acaacgccct gaaggcgctg
    22561 ggcgtcgaac gcctgagcct gctctcgccc tatcccgact ggctcaccac gctggccgcg
    22621 gcctactggc agagcgccgg tttccaggtc gaccacatca gggccttcga ggacaagctc
    22681 gtggcctata ccgtcacgcc cgcgcaagtc gccgaagccc tgcgcgatac cagggccgaa
    22741 cccggcggcg cggtggtgat gagcggcacc ggcatgcgca cgctggaagc gctggcgcag
    22801 gtgcaggcca agggcggaac gcccattctt tcgtccaatc tatgcagcgt atggagcctg
    22861 gtgcgcaacg tcaaggatat gacgtccgca ccctggatga gcgccgccct gccgtacgcg
    22921 ctgcgctgat tcaacacatt gcggaccggc atggtgccgg cccgcgacgg atgcaaaaac
    22981 cctaggggaa taaagtgatt gccatgccca agcagcaaac cagcggcctg cgtgccgccc
    23041 tggccgcgtt tgccgcggtc ggcctgatgg ccggcgccgg cgcccacgcc gaacaacccg
    23101 tgccgggcta tcccagcaaa tccatccgga tgatcgtgcc cttcccggcc ggcggcacca
    23161 ccgacgtcgt cgcccgcctg atcgccaagg gcatcaccac cgcgtggggc cagtccgtcg
    23221 tcatcgacaa ccggggcggc gccgcgggca tgatcggttc ggccgaaggc gcccgcgcgg
    23281 cacccgacgg ctacacgctg gtgatgggca acaaccagac ccacgccacc aacgcctcgc
    23341 tgttcgccaa gccgcagttc gacatcacca acggcgtaac gccgatcgcc atgctcaccc
    23401 gcaccaagca cgtgatagtg gtgcgcagcg actcgcccat caagacctac caggacctga
    23461 tcgccgcggg caaggaccat cacctgtcct tcggttcgcc cagcgccggc tcgtcctcgc
    23521 acatcatcag cgacgccatc cgccgcgcca cgggcatgga cgccacgcac atcccctaca
    23581 agggcgccgc gcccctgatg gtcgacctgc tgagcggcca gatcgatttc tccaccgcca
    23641 cctacggcag cgccgccagc tacatccacg acggcaagct gcgtccgctg gccgtcggcg
    23701 ccgaacagcg cgaccccgag ctgcccaatg tgcccacctt cgacgaactg ggcctgaagg
    23761 gcctgtcgct gtattcgtgg gtgggcctgt atgccccggc caataccccc gaacccatcg
    23821 tccgcgcctg gagcgacgcc gtggcccgga tcctgaagga tccggacgtg atcagcacgc
    23881 tgaagactgc cggtttcgag gtttggtgca agccctatac cgagatgaag accttccacc
    23941 ccgaagaaat caagcgctgg gccgatctgg tgaaggccgg cgggatttcg ctggattgat
    24001 ccgtgacgcg ccgatcaggc gcggttgaaa aacgggacgg cttcgcgaga agccgccccg
    24061 tttgcttttg ggcccttggg cgatgtgcgg aaaaggtgaa cggaatccct gccgtcttgc
    24121 cgggcgataa aaaaaacaga tatctaaatc gaggtttcgc aacgccggtc gggcgtccac
    24181 tgggcatact agaaaaggcg ttcacgccag ggcacgttca cgccagaaca acatggcggt
    24241 gcgaggagac aggcatgaag atttcaagaa gctgtacaga cggggactgc cgggctcggc
    24301 gatcatgctg cgccgcggca tcatgatcga gggtcacggg gcgttcctga ccgggcgcgc
    24361 ggcgtcagcg tccggccacc tcgcccgcga cctcgcggat tgcgtcgatc agtacccgcg
    24421 ccgccggcga gggcgtgccg ccggcgcgca aggtcaggcc gatgtcgcgc cgcaccttgc
    24481 gcaaggtcac gtccagcgcc acgagttgtc ccgaggcaag ttcgtagtgc agttgctgcg
    24541 cggaaagcac ggtgatcatg tcggtgtcga gcagcaggcc gcggatcacc gcaagatccg
    24601 ccgtctcgac cgcgggcatg ggcggggcga tcttcacgcg tcggaacaag gcgtcgaaca
    24661 ggccgcgcgc gggcgagtgc gcgcgcgaca ggatccattg cgcatgggtc aggtccttga
    24721 gggtcagccc gcgcacgccg gccagcgggt ggccgcgacg ggccaggatg gcgaggtttt
    24781 ccgacatcag cttttcattt tccaggccgc tggccgcggc attctcgcgc agcgcgccca
    24841 ggacgaagtc gatgtcgccc gcgcgcaggc cggcgaccag gggctcgtag gcgctttcat
    24901 cggtgaccac gcgcacgccg gggtagcggc cgatcacccg ggccacggcc tggggaagga
    24961 tcagcgtgcg tcccagcggc agcgcgccga ccgtcaccac gccctggatg ctgccgcgca
    25021 gggcggcgac gtcgtccgat acgtggcgca gttcattcag cgcgcggcgc acgtgcagca
    25081 ggaaggtctc gccgtcgttc gtcggcagca gtccgcgcgg gttgcggtgg aagaggagca
    25141 tcccggcgcc gttttcgagg atgcggatcg cgctgctgac ggcgggctgc gtgatgccga
    25201 gcgcgcgggc cgcgctcggc atgtggcgat ggcgcgccag cgccgtgagt atttgcaggc
    25261 gccgcgtatt gagcagatag gccggcgcgc cgccatcgct gaccgcctgc cagcgcgact
    25321 gccgggccgc gcaccagcgc gccagcgcat cgagttcgtc gaagacgcgt tcgctgcgct
    25381 tgagcacggt gccgcagacc ggcgtaggca gcatcccggt cggcctgcgg tcgaaaagcg
    25441 gcgcaccgag catggcctcg acttcctgca cggcacgcgt gacggccgac tgcgcgcgga
    25501 acaacgccat ggcggcgcgg cttacgctgc cggtttctgc gacctcccgc accgcgcgca
    25561 ggcaggccag gttgaggaat tcatcgttgt tcatgccggg gctttgcgcg agctgccaat
    25621 ggcaggttgt ttaccagggt attcatttta tttatgaacg agcaagtttt tggaaacggc
    25681 aatgatcatt gatatccacg gacactacac caccgcaccc aaggcgctgg aggcctggcg
    25741 caaccggcag gtcgccgcga tcggcagccc ttcggaaatg cccaaggccg ccgaattgcg
    25801 catttccgac gacgaattgc gcgaatcggt cgagacaaac cagctgcggc tgatgcgcga
    25861 acgcggcctg gacctcacga tcttcagccc gcgggccagt ttcatggcgc accacatcgg
    25921 cgatttccag gtgtcgagca cctgggccgc gatctgcaac gaactgtgct ggcgggtaag
    25981 ccagctcttt cccgacaact tcgtgccggc ggcgatgctg ccgcaaagcc ccggcgtgga
    26041 catcgccacc tgcatcccgg agctggtcaa gtgcgtcgag cagtatggca atgtggccat
    26101 caacctcaac ccggacccgt cgggcggcca ctggacgagc ccgcccctgt cggaccgcta
    26161 ctggtacccg atctacgaaa aaatggtcga gtacgacatc ccggccatga tccatgtgag
    26221 caccagctgc aatgcgtgct tccacaccac gggcgcccac tacctgaatg cggacaccac
    26281 cgccttcatg cagtgcctga cttccgactt gttccacgat tttccgacgc tgcgcttcgt
    26341 catcccgcac ggcggcggcg ccgtgccgta tcactggggg cgcttccgcg gcctcgcgca
    26401 ggaaatgaaa aagccgttgc tcaaggacca tctgctgaac aatatctttt tcgacacgtg
    26461 cgtctaccat cagccgggca tcaacctgct caccagcgtc attcccgtcg acaacatcct
    26521 gttcgccagc gagatgatcg gcgcggtgcg cggcatcgat cccgagaccg gccattattt
    26581 cgacgacacg aaacgctaca tcgagaccgc gcacctcgat ccggacgagc gctacaagat
    26641 ctacgaaggc aatgcgcggc gcgtctatcc gcgactggat gccaggctca agcgcaacgg
    26701 acactgaaca ggcgtgagcc ggctcgagtc cggacggcgc ttacccaata ttgctacaca
    26761 actggaaatc aacatgtcta cgacccttct ccgcgtgctt gaacaaccgc tgccccgcgt
    26821 cgatgacgcc ctcttgaagc gctatgccga ggtcggctgc gcgcagatca gcgactgcat
    26881 gaaccggctc tacggcgtgg tgagcctggc cccttaccat gacaccaagg ggccgcgcat
    26941 ggtgggcatc gccctgacgg tcaaggcgcg cccgggcgac aacctgttca tccacaaggc
    27001 cattaccctg gccaggccgg gagacgtcat cgtcgtcgac ggcggcggcg acctgaccaa
    27061 tgccctggtg ggcgagctca tggccatgga agccgagcgg cgcggcgtgc gcggcttcgt
    27121 catcgacggt tcggtacgcg atgccgatgt ctttgccaag aatggcaatt tcggctgctt
    27181 tgcccgcggc gtcagccacc gcggccccta caaggatggt cccggcgaaa tcaacgtgcc
    27241 cgtcagcatc ggcgggctgg tggtgcttcc tggcgacgtg gtggtcggcg atgccgatgg
    27301 cgtcatggcc attcccgccg gcatggccga gctcgtgctg ggcctggccg aaaagaaaga
    27361 agccgccgaa gtgcaaagca aggaaaagat ccgcaatggc accaatgcca ggccgtggat
    27421 agaccaggcc attgcaaggc tgaatggtca aggctgaaca agccgggccc gcggcgcttg
    27481 aaccgcgccg caagacggtg caagctgctt gcgctgacgc ccaaggaata agcgggcctg
    27541 gtcgatcgta tacatataaa ggaaaggaga agaaacatga ccaaagataa gcacaccctg
    27601 tttcgaatgg ctcgttcgtc gctgctgggc gccggcctcg ccctcgtggc ggccttgccg
    27661 gcgcatgccg acgatgccta tcccaacaag cccattcata tcgtcgtgcc gtatacgccg
    27721 ggcggggcgg tggacatcgt cacgcggctg gtgacgccga agatgggcga aatactcaag
    27781 cagtccatca tcgtcgacaa tcgtccgggc gccgcgacca atatcggcat ggattacgtg
    27841 acgcgccagc cggccgacgg ctacacgctg ctcacggcct cgcccagcct gtcctccaac
    27901 ggcgcgctgt tcaccaacct gcatttcgat ccggccaagg ctttcgcgcc ggtgggcgag
    27961 atcggctatg cgccgctggt ggtcgtggtc cccgcggcgt cgccctacaa gaccctcaag
    28021 gacctgatgg acgacggccg ggcgcatccc gagaaactgt ccttcggcac ggccggcaac
    28081 ggcagctcgg gccacctggc gggcgaactg ctcaaggccg agggcaagtt caatgccacg
    28141 cacattccct acaagggcgg ttcgccggcc atcaccgacc tgctgggcgg gcgcctggcg
    28201 ttcatgccga tcaacccgct ggaagtgatc tcgcacctga agtccggcag tctgcgcgcg
    28261 ctggccgtgc tcgacgccaa gccggcggcg ctgctgcccg atgtgccgac cgcggcgcag
    28321 caaggcgtgc ccggcgcgca ggccgcggta tggtggggcc tggaggggcc ggccggcatg
    28381 ccgcaagcgg tggtcgacaa gctcaatgcc gccttgcagg ccgcgctggc cgacccgatg
    28441 gttcagagcc gcatggccga cctgggcgcg acgctgaccc cgggcagcgc cgccgatttc
    28501 ggcaccttcg tcgcgaacga aaccgtgaag tggaccaagg tgatcaaggc ggccgacatc
    28561 aaggccgatt gaagtgcagc gatagcagcg ccgcggtccg gtgacgccgg caccgcggcg
    28621 ctcgaaaaca aaatcaaaga ggagaccggc catgccgaca cgcttgctga aaacgccata
    28681 ccgctatttt gcccacctgc tgtccctcgc ctgcctgctg acggccggcc tcgccagcgc
    28741 cagccccgcg gacgacgccg cgcgccaggc gctggcgcct tccggcgtgc tgcgcgtcgg
    28801 cctgtatcgc ggcagtccga cgtcgatcat cgagaacgcc gcttcgcacg agtcccgggg
    28861 cgtggcgtac gatctgggcc ggcagttggc cgcggccctg ggcgtgccct gcgagacggt
    28921 ggtctatccc gcgaacgcgc ccctgctcaa ggcgctggca tccagcgagg tcgacgtggt
    28981 cttcaccaac gccacggccg agcgcgcccg gcacatggac ttctcgccgg tcttcatgtc
    29041 ggtcgagaaa agcctgctgg tgccgggcgg ttcgtctctg aagaccctgc aggacgccaa
    29101 gcgcccgggt ttgcgggtcg gcgtgagcca gggcagcagc acgtccgagg agctgctcgc
    29161 gctctatccc ggcatgtcga cccagcccat cgataccctg cagcatgccg cgcagatgct
    29221 ggccagcggg cagatcgacg ctttcgggac caacgacgcg atcctctacc agatgtccga
    29281 cggcgtgcct ggctcgcggg tgctgccggg ccactggggc acggagacct tcgccgccgg
    29341 cattcccaag ggacgccagg ccggcatgcc tttcctgcgc ggcttcatgg ccgccgccga
    29401 ggccgatgga tccgtcgccc atgccgttgc gcgagccgac ctgcgcgggg tgctgccggg
    29461 ctcggcgcat tagggggcgg gcagcgcgga gggagatgaa ttgggtgtaa atgggtgtat
    29521 tattggatcc catgatttac accgattctc ttcaaatcac cccggaaatc ctgggcctgg
    29581 tcgctggcat cgacgagttc aagggtgcct ggcgcgcgct gggcgcgctg gcgcccgacc
    29641 ggctgtcggc gctgcggcgg gtcgccacca tcgagagcat cggttcctcg acccggatcg
    29701 agggcagcaa actgtcggac cgcgaagtcg aacgtttgct ctccaacctg cagatcaagt
    29761 cgttcgagac ccgcgacgaa caggaagtgg cgggctatgc cgaagtcatg gagctggtgt
    29821 tttcctcctg gcaggacatc gcgctgacgg agaaccacat ccggcaactg caccgcgacc
    29881 tgctggtcca cagcgacaag gatgcctggc accgcggcgg ctacaagacg tccaccaaca
    29941 gcgtggccgc gttcgacgaa cagggcaagc cgctaggcgt ggtgttcgag accgccacac
    30001 ccttcgatac gccgcgcctg atggccgaac tggtggcctg gttcaatgag gagcgcggca
    30061 cgggacgcct gcatcccctg ttgctcatcg gcatctgggt cgtggtgttc ctggagatcc
    30121 accccttcca ggacggcaac ggccgcttga gccgggtgct gaccacgctg ctgctgctgc
    30181 agaccggcta cgcctacgtg ccctacagct cgctcgaaag cgtgatcgag cagagcaagg
    30241 aggcctacta tctggcgctg cgccagaccc agggcaccat ccgcaccgcg gcgccgaact
    30301 ggcagccttg gttggcgttc ttcctgcgcg cactgtccca gcaggtgcgc cggctggaac
    30361 gcaaggtcga gcgcgagaag ctggtgctcg ccaagctgcc ggacctggcg ctgcaggtcg
    30421 tcgaattcac gcgcgagcat ggccgcgtca ccatggccga ggccatgcgc ttgaccggcg
    30481 gcaaccgcaa cacactcaag cagcacttcc gggcgctggt ggagcagggg cacctggtcc
    30541 agcatggcgg cggccgcggg acctggtatg cgttgcgctg acatgccgcc atggccggct
    30601 atggaccgcg aatcacttgc cgatgcagaa actggagaag atcttcccca gcaaatcatc
    30661 gctggtgaac tggcccgtga tcgccgtcag gctcgcgtgc gccaggcgca gctcctcggc
    30721 gaacagatcc agcacccggt cgtcctgcga ggcgtgctgg gcggcgagcg ccaggtgttc
    30781 cgccgccgcc tgcagcgcgt gcaggtggcg ttcgcgcgcc agccagggcg attcggcgcc
    30841 cgggttccag ccggccaggc gcagcagttc ggcgcgcagt tcggccaggc ccgcgcctga
    30901 acgggccgaa atgcccagcg cgccggcatg gccctggccg cccgtgggcg cggcaaaagg
    30961 ccagggcagc aggtccacct tgttgtagac ggccagcacc ggcgtgcggg ccggcaggcg
    31021 ggcggtgatc ccggcatcga gcgcgtcgcc cgggtgcgtg gcgtcctgca ggtgcaggat
    31081 cacgtcggcg cgttcgattt cctgccaagt gcgggcgatg cccaggctct cgacggtgtc
    31141 gacggtctcg cgcaggccgg cggtgtcgac gatgtgcagc ggcaccccgt cgatgtggat
    31201 ctcctgcacg accttgtcgc gcgtggtgcc ggcgatcggc
//
LOCUS       DASWPO010000188         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_756590, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000188 DASWPO010000000
VERSION     DASWPO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756590"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..752
                     /locus_tag="VGC69_09885"
     CDS             141..752
                     /locus_tag="VGC69_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY0295628.1"
                     /translation="MSIATNPTRIEMWFDFASPYSYLAMARLPALAREAGVPFALQPF
                     LLGPIFQAQGWNDSPFRLFPGKGAYMMRDIVRLADKYGVAYKRPDVFPRVGVLPTRVA
                     LLGQNQPWGVDFCLAAFQANFVHDRDIQSGDTMADLLRGLSLDAEALIEQARHEDAKE
                     ALRQQVDRARDLGIFGAPMFLVGGEMFWGNDRLEDAIDWAMKH"
     gene            761..1516
                     /locus_tag="VGC69_09890"
     CDS             761..1516
                     /locus_tag="VGC69_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0295629.1"
                     /translation="MAVESRVMLVTGAGRGIGAAVARLAAQRGYAVCVNYHQRREPAE
                     AVVQAIRAAGGTALAVQADVAREDRILALFGQVDEAFGRLDVLVNNAGVLDLQTTVEQ
                     MSAARIGRILATNVLGPFLCCREAVRRMAPRHGGRGGAIVNVSSMAARLGSPNEYVDY
                     AASKGAVDTLTIGLAKEVAGDGIRVNGVRPGLIHTGMHASGGEPGRVGRMKDGIPLKR
                     GGTAEEVANAVLWLCSDEAAYTTGSFIDVAGGR"
     gene            complement(1518..1847)
                     /locus_tag="VGC69_09895"
     CDS             complement(1518..1847)
                     /locus_tag="VGC69_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEY0295630.1"
                     /translation="MNKVTATPAALRLIDELTAQYGPIMFHQSGGCCDGSSPMCYPLG
                     ELLVGDSDVLMGEIGGAPFYIGAAQYQAWKHTDLIIDVVPGRGGMFSLDNGREERFLT
                     RSTVCMT"
     gene            complement(1919..>2050)
                     /locus_tag="VGC69_09900"
     CDS             complement(1919..>2050)
                     /locus_tag="VGC69_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011748649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0295631.1"
                     /translation="AHAAFGGYKQSGIGRENHLMMLNHYQQTKNLLVSYSPKKLGFF"
BASE COUNT          315 a          689 c          714 g          332 t
ORIGIN      
        1 tttccacagc cagggtttcg gccgcgccgc cgtcatcggt gaaatgcggg ccggcgagcc
       61 ggccgtgcac gtagcatgac ccataaggcc atgcgccgcc agccggcgcg tggcgaggtt
      121 cgagatttcg ggagttttgc atgagtatcg cgacgaaccc cacccgcatc gagatgtggt
      181 tcgactttgc cagcccctac agctacctgg ccatggcgcg cctgccggcg ctggcccgcg
      241 aggccggcgt gccgttcgcg ctgcagcctt tcctgctggg gcccatcttc caggcccagg
      301 gctggaacga cagccctttc cgtctctttc ccggcaaggg cgcctacatg atgcgcgaca
      361 tcgtgcgact ggccgacaaa tacggcgtcg cctacaagcg gccggacgtg ttcccgcgcg
      421 tgggcgtgct gcccacgcgc gtggccctgc tgggccagaa ccagccctgg ggcgtggatt
      481 tctgcctggc ggccttccag gccaacttcg tgcacgaccg cgacatccag tccggcgaca
      541 cgatggcgga cctgctgcgg ggcctgtcgc tggatgccga ggccttgatc gagcaggcac
      601 gccacgaaga cgccaaggaa gccctgcgcc agcaggtcga ccgcgcccgc gacctgggca
      661 ttttcggcgc gcccatgttc ctggtcggcg gcgagatgtt ctggggcaac gatcgcctgg
      721 aagacgcgat cgattgggca atgaaacact aggagccgcg atggccgtcg aatccagggt
      781 catgctcgtc accggcgcgg gccggggcat aggcgccgcc gtggcgcggc tggcggcgca
      841 gcgcggctat gcggtctgcg tcaattacca ccagcgccgc gaaccggccg aggccgtggt
      901 ccaggccata cgcgcagcgg gcggcacggc gctggccgtc caggccgacg tggcgcggga
      961 agaccggatc ctggccttgt tcgggcaggt ggacgaggcc ttcggccggc tcgatgtcct
     1021 ggtcaacaat gccggcgtgc tcgacctgca gaccacggtc gagcagatga gcgcggcgcg
     1081 catcggccgc atcctcgcca ccaatgtgct cgggcctttt ttgtgctgcc gcgaagcggt
     1141 gcgccgcatg gcgccgcgcc atggcggccg cggcggggcc atcgtcaacg tgtcgtccat
     1201 ggcggcgagg ctgggttcgc cgaacgaata tgtggactac gccgccagca agggggcggt
     1261 ggatacgctc acgataggcc tggccaagga agtcgccggc gacggcatcc gcgtcaacgg
     1321 cgtgcgcccg ggcctcatcc acaccgggat gcacgccagc ggcggcgagc ccggccgggt
     1381 cggccggatg aaggacggca ttccgctcaa gcgcggcggc accgccgagg aagtggcgaa
     1441 cgcggtgctg tggctgtgct cggacgaggc cgcctacacc acggggtcgt tcatcgacgt
     1501 ggccggcggc cgataggtca cgtcatgcaa accgtcgagc gcgtcaggaa gcgctcttcc
     1561 cgcccgttgt ccagcgagaa catcccgccc cgtccgggca ccacgtcgat gatcaggtcg
     1621 gtgtgcttcc aggcctggta ctgcgccgcg ccgatgtaga agggcgcgcc gccgatctcg
     1681 cccatcagca cgtcgctgtc gcccaccagc agctcgccca ggggatagca catgggcgaa
     1741 gagccgtcgc agcagccgcc cgattgatgg aacatgatgg ggccgtactg cgccgtcagc
     1801 tcgtcgatga gccgcagcgc cgccggcgtg gccgtgactt tgttcatgag ggttcctttc
     1861 gactgctcat ccgaagggcc gggcacgggc tgcgccatgg cccgtgcccg ccgtccggtc
     1921 agaagaagcc gagcttcttc gggctgtagc tcaccagcag gttcttggtt tgctggtaat
     1981 ggttgagcat catcaggtgg ttttcacgcc cgatgcccga ctgcttgtag ccgccgaagg
     2041 ccgcatgcgc
//
LOCUS       DASWPO010000189        17133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_131255, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000189 DASWPO010000000
VERSION     DASWPO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 17133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17133
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131255"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VGC69_09905"
     CDS             complement(<1..337)
                     /locus_tag="VGC69_09905"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEY0295632.1"
                     /translation="MSDPDFSSSIAPTPRRALVLFSGGQDSTTCLAWALARYDHVETV
                     AFDYGQRHRIELDARLNVLRELRAAFPRWTARLGEDHLLDLSVLGQVGDTALTSDRKI
                     EMQANGLPNT"
     gene            414..3293
                     /gene="ppc"
                     /locus_tag="VGC69_09910"
     CDS             414..3293
                     /gene="ppc"
                     /locus_tag="VGC69_09910"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY0295633.1"
                     /translation="MTTELPHADVSLRHTIRLVGTLLGEVIVACEGRQVFDTIETLRR
                     TAVRFRRQGRTADSKILQDSVRRLRGSNPNDVARAFSYFLHLWNIAEDRDQTRQQREQ
                     VLRGADPARGSLRDTMARLKAQGITPSQIRRLLANACIMPVLTAHPTEVQRKSTLDQH
                     QAISALLRRREQPLADDEQAQVQEALLGRIATLWQTRMLRHTRLTVADEIDNALSYYR
                     STFLSVIPRLYADLDKLLRPAAKPFDPPAPPMTPFLRMGSWIGGDRDGNPNVDAGTLD
                     HALRRQSTVLLEHYLQEIHAIGATLSVSTLLIGVSPALQALSDASGDDSPHREDEPYR
                     RALIDMYARLASTAQRLIGETLALRRTHAAAPYAGPAEFARDLAVVADSLAKHHGAPI
                     ARLRLTGLQQAVAVFGFHLATVDLRQSSDVHERVLAELFTRADAAWQGKPLVYTALSE
                     DERVALLRNELAHARPLASPWIAYGEETTRELAILRTAAACRARYGKDAVRQTIVSHT
                     ETLSDLLEAMVLQKEAGLLASPPDAGASAPQGESGPAPDRSLDGLMVVPLFETIPDLE
                     RGAGIMAAWLDLPEIRERVHYAQGGAQEVMLGYSDSNKDGGFLTSNWALYHAERALVE
                     VFGARGVALRLFHGRGGSVGRGGGSSFEAILAQPPGTVAGQLRLTEQGEVIQSKYKDA
                     EIGRWNLELLLTATLESSLAPQAEAARAEAAHMEDYGPAMSFLSDQAQRTYRGLVYDT
                     PGFAEYFFASTPISEIAGLNIGSRPSSRKPSQRIEDLRAIPWGFSWAQCRLMITGWYG
                     VGSAFEHYLETGAPGAPRSKKARLALLREMAETWPAMRTLLSNMEMVLAKSDLAIGAR
                     YAELVPRRALRERVFGMIAAEHERTLSMLRLLTRRELLADNPGLHASLHERFAYIDPL
                     NYLQIELLRRWRAGPAKPGKTPDPRVRSAIHLTINGIAAGLRNSG"
     gene            3396..4085
                     /locus_tag="VGC69_09915"
     CDS             3396..4085
                     /locus_tag="VGC69_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY0295634.1"
                     /translation="MINKIVSSTAEALAGVPDGATVMIGGFGTAGQPIELIDALLEQG
                     AKDLVIINNNAGNGTAGLAALLAANRVRKIICSFPRQVDSQIFDGLYRGGKIELELVP
                     QGNLAERIRAAGAGIGAFFTPTGYGTPLAEGKETREIDGRHYVLEYPLHADYALIKAE
                     CGDRWGNLTYRKTARNFGPIMASAAKVAVVQVSKVVELGELDPESVATPGIFVQRIVQ
                     IDAPRGVQEQK"
     gene            4082..4723
                     /locus_tag="VGC69_09920"
     CDS             4082..4723
                     /locus_tag="VGC69_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY0295635.1"
                     /translation="MSHPLSRDQIAARVARDIPEGAYVNLGIGLPTLVANHLPAGREV
                     ILHTENGMLGFGPAPEKGQEDYDLINAGKQPVTELPGCSYFHHADSFGMMRGGHLDIC
                     VLGAFQVSRHGDLANWHTGAPDAIPAVGGAMDLAIGAKDVYIMMELTTREGRSKLVEA
                     CTYPLTGVRCVSRVYTDMAVFDLKNGEITVVDRFGDATPGSLLALTGLPLKFS"
     gene            4858..5457
                     /locus_tag="VGC69_09925"
     CDS             4858..5457
                     /locus_tag="VGC69_09925"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEY0295636.1"
                     /translation="MNARFAALLLLALASGPACAQNLRHDPAVYALTATRDLTLGQTT
                     ITGSYTNGGYATQPAPKITFALYDADDHEVGRLIEHAEADLAPGQTWDIRATTPLLFA
                     RYLPMTDAPPPVAAPVSLGVRRAPRRSPALAKAPATTANPAPKKTVIYRKPELYDWSK
                     HHRPVSTTAHGSTVRRASPAVRPGYVPRKNAAPSTRSVR"
     gene            complement(5583..5918)
                     /locus_tag="VGC69_09930"
     CDS             complement(5583..5918)
                     /locus_tag="VGC69_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY0295637.1"
                     /translation="MASLSRKLAAAAVVAAFSAAPVWAQTAAPAKTLTPQQQRMNDCN
                     SKASGQALKGDARKSYMSSCLRGETPAPVGKPLTPQQQKMKDCNGQAGAQSLSGEARK
                     TFMSSCLKK"
     gene            6132..6785
                     /locus_tag="VGC69_09935"
     CDS             6132..6785
                     /locus_tag="VGC69_09935"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY0295638.1"
                     /translation="MFPKQLSDGYQSFLTGRLPSERSRYQALAERGQQPEVLIIGCCD
                     SRVSPEVIFDAGPGELFVIRNVANLVPPCETDTASSSYHGTSAAIEFAVNGLNVKHIV
                     VLGHASCGGIRSFYDDGEPLSKLDFIGKWMSQIQPVADRLGPGTGDRDADIKRLELGV
                     IEHSLANLLTFPSIRRRVDTGELQLHGAYFGVATGMLFVRDPVTRGFTPFTTLNPQD"
     gene            7006..8256
                     /locus_tag="VGC69_09940"
     CDS             7006..8256
                     /locus_tag="VGC69_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY0295639.1"
                     /translation="MAGLILGILLGLASYYSFPDKATAAEVAKYMSLVSSIFLKLIKM
                     IIGPLVLSTLIVGIGHMGDAATVGRVGAKTMAWFVCASIVSLLLGLFMVNLLQPGVGI
                     AIADQGAAVANLSTSAFSIENFVDHIVPTSIFKSLADGEILQIVVFSIFAGVAIMAMG
                     ERGKPLIELADNISHMMLVITGYVMKLAPIAVCASVASVITKSGPGILLNFAKFLGGF
                     YLTLIMLWLILVVVGYLTLGSRMGNLVKRMYEPFLLAFSTASSEASYPKILDQLEKFG
                     VSKKIASFVLPLGYSFNLDGTMAYCTFATLFIAQAYHIDMPISQQLAMAAVLMLTSKG
                     VAGVPRASLVVILATLKQFGIPEAGLLLILGIDQFLDMGRSATNVIGNSLAASAVAKF
                     EKALGPAQDEAPAAADGAPRPTAS"
     gene            8572..10314
                     /locus_tag="VGC69_09945"
     CDS             8572..10314
                     /locus_tag="VGC69_09945"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY0295640.1"
                     /translation="MYVYDEVDQQLVDERVAQFADQTRRFLDGQLTEDDFRVLRLQNG
                     LYVQKHAPMLRIAIPYGMLAARQLRTLAHVARTWDRGYGHFSTRQNLQLNWPRLEDVP
                     AILAELARVQMHAIQTSGNCIRNTTTDHFAGVAPDELVNPLVWCEIVRQWSALNPEFA
                     FLPRKFKIAVSGAIEDRAAVGVHDLGLQAVERDGKLGFRVWVGGGLGRTPMVSHLLQP
                     FMEWQHLLTYLHAVLQVYNLHGRRDNKYKARIKILVKDLTPDVFAQQVDEAWQLLKNG
                     PDTVTQEYVDAIAARFTQPGYDAQAAGEPDETEARAAENPSFAHWLRRNTHPHKVPGY
                     AAVTLSLKPHGAPPGDITADQMDAVADLSERYGFGELRVSHEQNLILPDVRRSQLHAL
                     WQELRELKLATANIGLLTNIIACPGGDFCALANAVSIPVAQAIQERFDNLDYLFEIGE
                     LDLNISGCINACGHHHVGHIGILGVDKAGEEWYQVTIGGRQNGAARPLPDLESTRGVG
                     AAIGRIIGPSFARATVPLVVDCLIRTYLGLRESDAERFIDVVDRLGIEPFKHDVYADP
                     EVIKAAEQREVAHA"
     gene            10307..10837
                     /locus_tag="VGC69_09950"
     CDS             10307..10837
                     /locus_tag="VGC69_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY0295641.1"
                     /translation="MPDAPIATAPAPSLIRNGGIEADAWQVFQPTENSALPADEAGWI
                     VPLPAWLSSPAALRARRHPVGVLLPSDTELKDWAGEAGKPGKIDFTGIAFIAVDFPVY
                     TDGRGYTLAQQLRRQYGYAGELRAVGDVLIDTIHYQARVGFDSFLVKPGHDPAKALAA
                     LHTFSVHYQKAYRVPA"
     gene            complement(10856..11635)
                     /locus_tag="VGC69_09955"
     CDS             complement(10856..11635)
                     /locus_tag="VGC69_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0295642.1"
                     /translation="METTDSGPCVLRRGLRVLGVLRQAGMDSLHVVEIARAAGMQRST
                     VYRYLDVLVEEGYALRDQDGPRYRLADSEIAPAIDPHVQAIRQLQPVLRQISDELGDS
                     SFLICRAGDDALCLHRELGSYPVQVLSVPVGHRQPLGVGSAGLALLAALPPAEAGEVI
                     ASNGLALHAYGGMTAGKMHKLVDNTRARGWAVVGNSAVPGVLGVGVALCDSKGYPRFS
                     ISVSSLIDRMPTPRQHAIAELIRNALKSSSDGRYGLNAQGV"
     gene            11764..12750
                     /locus_tag="VGC69_09960"
     CDS             11764..12750
                     /locus_tag="VGC69_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295643.1"
                     /translation="MNKPRTRMLGALAVAGALASPAPAPAANAYPQHPITIVVGYAAG
                     GATDIAARLISKPLSEILGQSVLVENKTGANSNIGAEVVTRSQPDGYTLYLGSIANTI
                     NQTLYAKLNYDFVKDFKPVGLLATIPNILVVNPKLPIKTVQDYIAYAKSHPGKLSCAS
                     SGTGSSIHLSCELFKMETGTNILHVPYRGSGPAVVDLLGGQVDSMFDNLPSSLPQVQA
                     GKLRAIGVTSPQRVPTAPDVPTIAESGLPGYHVQSWFGLVAPAGTPQAVIERLNAALN
                     KALAQPGVQALYAQSGFSLPESPNSPETFGHLISTEIDQWGKVVKTAGITTE"
     gene            12766..14268
                     /locus_tag="VGC69_09965"
     CDS             12766..14268
                     /locus_tag="VGC69_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0295644.1"
                     /translation="MYPIDFFWRAAERHPDRIALDGPDGRLTYQALASRVNALAAALQ
                     TLDPQPQTRVAICAGNSARHLIALLATLASGKVWVPLNYRSTPHEISRIIDATEPSIV
                     MVDASGEALVPAAPHAYLRLHAGDAHGLDALIASHAGDQPARHDLPRDATQAIKFTGG
                     TTGMPKGVMQPYRAWNTNIINQIDAWQLTPEDRYVMAAPVTHGTGTYLLPVLARGGTH
                     LLLDGTSPATVTQAFRERGGTLSFMPPTLIYMLMAQPGVTQADFPHLRNLIYGGAPMP
                     ADKVDQVRDFFGPVLATSYGQTEAPQLVTVMRPCDFEAPANHGSVGCVTWLSDMAVMG
                     PDGRLLPRGQIGEIVVRGDLVMTGYWRLPEKTAETLVDGWLRTGDGGLIDERGYLFLK
                     DRLRDVIITGGFNVYPVDVENALSTHPAVYECSVFGIPDEKWGEAVHAAVQFHPGAEA
                     TDDELKAHVRALLGPVQTPKHIHVHRSLPRSSVGKVLKTAVRDAALKETP"
     gene            14265..15050
                     /locus_tag="VGC69_09970"
     CDS             14265..15050
                     /locus_tag="VGC69_09970"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEY0295645.1"
                     /translation="MTTPETHLCAHHLTGMYYRDADLVNDLIGEQTFVQVLFSQIMGR
                     EARPVDLRIVDAVLITLMEHGLTPSAIATRLIYMSAPENLQGAVAAGLMAVGSQFVGT
                     MENCSRLLDRINQAADPRAEAQAIAGEYRADKRALPGFGHHLHKPDDPRAVKLLSLAE
                     AEPDLDQTHVHALRLLAGAVDAAHGRHITINATGAVAALLGGIGVPTALMRGFAVISR
                     AAGLVSHVAEEQQSPSGRFIWETIDHAIPYVGAGKSHQPGKQP"
     gene            15047..15772
                     /locus_tag="VGC69_09975"
     CDS             15047..15772
                     /locus_tag="VGC69_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0295646.1"
                     /translation="MKPHALVTGGSQGIGRAIVQRLIHDGMHVLNIDRCVPEAPLQGE
                     THVRLDLTDVGAVREAIAGIARGTPILRLVNNAGIVHPAPLEDASPEDLDAVVALNLR
                     APMLLMQGLLPVMRAHGFGRVVNIASRAALGKSERTVYAATKAGLLGMTRTWALECGA
                     HGITVNAVGPGPIASALFDRVNPPGAPQTERIIATIPLRRMGRPDEVAHAVASLLDER
                     AGFITGQVLYVCGGMTVGLGNAA"
     gene            15769..16581
                     /locus_tag="VGC69_09980"
     CDS             15769..16581
                     /locus_tag="VGC69_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0295647.1"
                     /translation="MTTRQDDRLSGKVAFVAGAGSSAAGWSIGKASCVTLARRGAMVV
                     ALDCDLAAAQDAAHEVESAGGRALPVQADIADPHAMRAAVDAALRHFGRIDILQANAG
                     IGKVGGPEDISLEDWNRIQQVNVTSLLIAMNLFAPIMRRQGGGAVVAVSSVAGIRYTG
                     YPHLAYSVSKAAVIHFARMAAQQYAADGIRVNTVIPGLIDTPRVARNVARMFDPDDLQ
                     AARTARDRQVPMGRMGTPWEVADAVAFLASDEASYITGTELLVDGGLTGKYA"
     gene            complement(16646..>17133)
                     /locus_tag="VGC69_09985"
     CDS             complement(16646..>17133)
                     /locus_tag="VGC69_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0295648.1"
                     /translation="GVLSIDIARSPMLWFTGYWQQDTPAIDGGYYRTGDTVEFEPDGS
                     VSFIGRSDDVITSAGYRIGPFDVESALIEHPAVMEAAVVGVPDPERTEIVKAYVVLSQ
                     GVEASPALAEELSNYVKKRLSAHAYPRLVEFVEGLPKTPSGKVQRFILRKMSAGEPGS
                     Q"
BASE COUNT         2950 a         6259 c         5317 g         2607 t
ORIGIN      
        1 aagtgttggg caatccgttg gcctgcatct cgatcttgcg atcggaagtc agcgcggtgt
       61 cgcccacctg gcccagcacg ctcaggtcca gcaggtgatc ctcgcccagc ctggccgtcc
      121 accgcggaaa cgccgcgcgc agctcgcgca gcacgttcag gcgcgcgtcg agctcgatgc
      181 ggtggcgctg cccgtaatcg aaggccacgg tctcgacatg gtcgtagcgc gccagcgccc
      241 aggccaggca agtggtggaa tcctggccgc ccgagaacag caccagggcc cgccgcggcg
      301 taggggcgat ggaagaggaa aaatcgggat cgctcatgcg ggcaatgtaa cgcaggcgga
      361 gcgggtgcgt acaatggccc ggcgcttgcg cgccctatcc gatggaacgc cccatgacga
      421 ctgaactccc gcacgccgat gtctccctgc gccacaccat acgcctcgtc ggcacgctgc
      481 tgggcgaggt gatcgtcgct tgcgagggcc ggcaggtgtt cgacaccatc gagacgctgc
      541 gccgcaccgc ggtgcgcttt cgccgccagg gccgcacggc cgacagcaag atcctgcagg
      601 acagcgtgcg ccgcctgcgc ggcagcaacc ccaacgacgt ggcgcgcgcg ttcagctatt
      661 tcctgcacct gtggaacatc gccgaagacc gcgaccagac ccgccagcag cgcgagcagg
      721 tgctgcgcgg cgccgacccg gcccgcggca gcctgcgcga cacgatggcc cgcctgaagg
      781 cccagggcat caccccctcc cagatccggc gcctgctggc caacgcctgc atcatgcccg
      841 tgctcacggc gcaccccacc gaagtgcagc gcaagagcac gctggaccag caccaggcca
      901 tcagcgccct gctgcggcgg cgcgaacagc cgctcgccga cgacgaacag gcgcaagtgc
      961 aggaagcgct gctgggccgc atcgccacgc tgtggcagac ccgcatgctg cgccacaccc
     1021 gcctgaccgt ggccgacgaa atcgacaatg ccctgtccta ctatcgcagc accttcctgt
     1081 cggtcatccc gcgcctgtat gccgacctgg acaagctgct gcgcccggcc gccaagccct
     1141 tcgatccgcc ggccccgccc atgacgccct tcctgcgcat gggcagctgg ataggcggcg
     1201 accgcgacgg caaccccaat gtggatgccg gcacgctgga ccacgcgctg cgccgccagt
     1261 ccacggtatt gctggaacac tatctgcagg aaatccacgc catcggcgcc acgctctcgg
     1321 tcagcacgct gctgatcggc gtgtcgcccg ccctgcaagc gctgtccgac gccagcggcg
     1381 acgattcgcc ccatcgggaa gacgagccct accgccgcgc gctgatcgac atgtacgcgc
     1441 ggctggccag caccgcgcag cgcctgatcg gcgaaacgct ggcgctgcgc aggacgcatg
     1501 cagccgcccc ctacgccggc ccggccgagt tcgcacgcga tctggcggtg gtggccgact
     1561 cgctggccaa gcaccatggc gcccccatcg cgcgcctgcg cctgaccggc ctgcagcagg
     1621 ccgtggcggt gttcggtttc cacctggcca cggtcgacct gcgccagagt tccgacgtcc
     1681 acgaacgcgt gcttgccgag ctcttcacgc gcgccgatgc cgcatggcag ggcaagcccc
     1741 tggtttacac ggcattgagc gaggacgagc gcgtcgccct gctgcgcaat gagctggccc
     1801 atgcccggcc gctggcctcg ccctggatcg cctacggcga ggaaaccacg cgcgagctcg
     1861 ccatcctgcg cacggccgcc gcctgccgcg cgcgctacgg caaggatgcc gtgcgccaga
     1921 ccatcgtctc gcacaccgaa acgctgagcg atctgctcga agcgatggtg ctgcagaaag
     1981 aagccggatt gttggcgagc cccccggatg ccggcgcgtc agccccccag ggggaaagcg
     2041 gcccggcgcc ggatcgctct cttgacggcc tgatggtggt gccgctcttc gagaccatcc
     2101 ccgacctgga gcgcggcgcc gggatcatgg ccgcctggct ggacctgccc gaaatccgcg
     2161 agcgcgtgca ctatgcgcag ggcggcgcgc aggaggtcat gctgggctat tccgacagca
     2221 acaaggacgg cggctttctc acgtcgaact gggccctcta ccatgccgag cgcgcgctgg
     2281 tcgaggtatt tggcgcgcgc ggcgtggccc tgcgcctgtt ccacggccgc ggcggctcgg
     2341 tgggccgcgg cggcggttcg agcttcgagg ccatcctggc gcagcccccc ggcaccgtgg
     2401 ccggccagtt gcgcctgacc gagcagggcg aagtcatcca gagcaaatac aaggacgccg
     2461 agatcggccg ctggaacctg gagctgctgc tgaccgccac gctcgagtcc tcgctcgccc
     2521 cgcaggccga ggccgcccgg gccgaggccg cccacatgga agactacggc ccggccatgt
     2581 cctttttgtc cgaccaggcg cagcgcacct accgcggcct ggtctacgac acgccgggct
     2641 tcgcggaata cttcttcgcc tccacgccca tcagcgagat cgccgggctg aacatcggct
     2701 cgcgcccctc gtcgcgcaag cccagccagc gcatcgaaga cctgcgcgcc attccctggg
     2761 gcttctcatg ggcccagtgc cggctgatga tcaccggctg gtacggcgtg ggttcggcct
     2821 tcgagcacta tctggaaacc ggcgcgccgg gcgcgccgcg ctcgaagaag gcgcgcctgg
     2881 ccctgctgcg cgagatggcc gaaacctggc cggccatgcg caccctgctg tccaatatgg
     2941 aaatggtgct ggccaagtcc gacctggcca tcggcgcccg ctatgccgag ctggtgccca
     3001 ggcgggcctt gcgcgaacgc gtgttcggca tgatcgcggc cgagcacgag cgcaccctgt
     3061 cgatgctgcg cctgctgacc cggcgcgaac tgctggccga caacccgggg ctgcatgcgt
     3121 cgctgcatga gcgcttcgcc tacatcgacc cgctcaacta cctgcagatc gaactgctgc
     3181 gccgctggcg cgccggcccg gccaaacccg gcaagacgcc agacccgcgc gtgcgcagcg
     3241 ccatccacct gaccatcaac ggcatcgcgg cgggcctgcg caactccggc tagcacagcc
     3301 cgcattcggg cagaatcagg cagaatcgac ataaaacgcc cacccgagca ttaaaaatac
     3361 cgagacatgc agcctttctt cgcgcagaca tccccatgat caacaaaatc gtatccagca
     3421 cggccgaggc gctggctggc gtgcccgacg gcgccaccgt catgatcggc ggcttcggca
     3481 ccgccggcca gcccatcgag ctgatcgacg ccctgctcga gcagggcgcg aaagacctgg
     3541 tcatcatcaa caacaacgcc ggcaacggca ccgccggcct ggccgccctg ctggccgcca
     3601 accgcgtgcg caagatcatc tgctcgtttc cgcgccaggt cgactcgcag atcttcgacg
     3661 gcctgtaccg cggcggcaag atcgagctcg aactcgtgcc gcagggcaac ctggccgagc
     3721 gcatccgcgc cgccggcgcg ggcatcgggg ccttcttcac gcccaccggc tacggcaccc
     3781 cgctggccga aggcaaggaa acgcgcgaga tcgacggccg ccactacgtg ctcgaatacc
     3841 ccctgcatgc cgactacgcc ctgatcaagg ccgagtgcgg cgaccgctgg ggcaacctca
     3901 cctaccgcaa gaccgcccgc aacttcggcc ccatcatggc gagcgccgcg aaagtggccg
     3961 tggtccaggt gagcaaagtg gtcgagctgg gcgagctcga cccggagagc gtggcgaccc
     4021 ccggcatctt cgtccagcgc atcgtgcaaa tcgacgcccc gcgcggggtc caggagcaaa
     4081 aatgagccat ccactttccc gcgaccagat cgccgcgcgc gtcgcccggg acatccccga
     4141 aggcgcctat gtgaacctgg gcatcggcct gcccacgctg gtggccaacc acctgcccgc
     4201 cggccgtgaa gtgatcctgc acaccgagaa cggcatgctg ggcttcggcc cggcgccgga
     4261 aaagggccag gaagactacg acctcatcaa tgccggcaag cagcccgtca ccgagctgcc
     4321 cggctgctcg tacttccatc acgccgactc cttcggcatg atgcgcggcg gccacctgga
     4381 catctgcgtg ctgggcgcct tccaggtgtc gcgccacggc gacctggcca actggcacac
     4441 cggcgcgccc gacgccatcc ccgccgtggg cggcgccatg gacctggcca ttggcgccaa
     4501 ggacgtctac atcatgatgg aactcaccac ccgcgaaggc cgcagcaagc tggtcgaggc
     4561 ctgcacctac ccgctcaccg gcgtgcgctg cgtctcgcgc gtctacaccg acatggccgt
     4621 gttcgacctg aagaacggcg agatcaccgt cgtggacagg ttcggcgacg ccacccccgg
     4681 ctccctgctc gcgctcaccg ggctgccgct caagttcagc tgagggcggc ggatccgcgt
     4741 gccgcgccgg cctgttacac ccccgttccg ggaaagcgct taaattttgg gccgagctgc
     4801 cggtaatacc tttacgccgg tggctgcaat gccgccggca ccttaccgga gcctgacatg
     4861 aatgcccgtt ttgctgccct gctgctgctt gccctggcgt ccgggccggc ttgcgcgcaa
     4921 aacctgcggc acgacccggc cgtctatgcc ctgacggcca cgcgcgacct caccctgggc
     4981 cagaccacca tcaccggcag ctataccaac ggcggctacg ccacccagcc ggcgcccaag
     5041 atcaccttcg ccctgtacga cgccgacgac cacgaggtcg gccgcctcat cgagcatgcc
     5101 gaggccgacc tggccccggg ccagacctgg gacatccgcg ccaccacgcc gctgctgttc
     5161 gcccgctacc tccccatgac cgacgcgccg ccgccggtcg ccgcgcccgt cagcctcggc
     5221 gtgcgccgcg cgccgaggcg cagtcccgcg ctcgccaagg cccccgccac gaccgccaat
     5281 ccggccccca aaaagacggt gatctatcgc aagcccgagc tctacgactg gagcaagcac
     5341 caccggcccg tcagcaccac cgcgcacggc agcacggtgc ggcgcgccag ccccgcggtg
     5401 cggcccggtt acgtgccccg caagaacgcg gcgcccagca ccagatccgt ccgctgagac
     5461 gacgcaagcc cccacgctgc gccttcggct tgccgccccc ggagggggcc gttttttgcc
     5521 ttgggtcggc ccggcggcaa aaagggcccc gcgaggggcc cttcacatgc aatggccagc
     5581 gcttacttct tcaggcagga gctcatgaaa gtcttgcggg cctcgcccga cagcgactgg
     5641 gcgccggcct gcccattgca gtccttcatc ttctgttgct gcggggtcag gggcttgccg
     5701 accggcgcgg gggtctcgcc gcgcaggcag gagctcatat aggacttgcg ggcatcgccc
     5761 ttgagcgcct gcccgctggc cttgctgttg caatcgttca tgcgctgctg ctggggcgtc
     5821 agcgtcttgg cgggagcggc cgtctgggcc cagacgggcg cggcggagaa ggccgccacg
     5881 acggcagcag cggccagttt gcgggaaaga gaagccatgt atcctccgag gcgcattccg
     5941 tccggacgaa ggcccgaagg gtgcggactg cgcagacagc cggcgctttg taaccggcgc
     6001 gccaatattc gggcaaattc ccctaccgtg caaggtgctt tacgtgaaga aatgacgtcc
     6061 gccgcgccga gccggaacga aagccgtcaa tatgggaaaa tccgccgacg ccccgcaacc
     6121 caaaggccct catgtttccc aagcaactct ccgatggcta ccagtccttc ctgaccggcc
     6181 gcctgcccag cgaacgcagc cgctaccagg ccctggccga acgcggccag cagcctgagg
     6241 tgctcatcat cggctgctgc gattcgcgcg tgtcgcccga ggtcatcttc gacgccggcc
     6301 cgggagaact gttcgtgatc cgcaacgtgg ccaacctcgt gcccccttgc gaaaccgata
     6361 ccgccagttc ctcctaccac ggcaccagcg cggccatcga attcgcggtc aacggcctga
     6421 acgtcaagca catcgtcgtg ctgggccatg cctcgtgcgg cggcatccgc tcgttctacg
     6481 acgacggcga accgctctcc aagctggact tcatcggcaa gtggatgtcg cagatccagc
     6541 ccgtggccga ccggctgggc cccggcacgg gcgaccgcga cgccgacatc aagcgcctgg
     6601 aactgggcgt gatcgaacac agcctggcca acctgctcac ctttccgtcg atccgccgcc
     6661 gggtcgacac cggcgaactg caactgcacg gcgcctattt cggcgtggcc accggcatgc
     6721 tgttcgtgcg cgatccggtc acgcgcggct tcacgccctt caccaccctg aacccgcagg
     6781 actagcgtcc agtacggctc gccccttgcc gccgggacgc gggaaaacgc gagttgtagg
     6841 gaagacagcc ttatgtcaat tttgtgtctc cggctacggc gataggcggc acgcatgatt
     6901 ccgtccctct ccgggcctca tcgcaggcaa acgtgatgga acccgcgcgc cgcaacggcc
     6961 gcgggcggac aggaggctgg cttgaacaag aatttcctct atgtgatggc cgggctcatc
     7021 ctcggcatcc ttctcggcct tgcgagctac tactcctttc ccgacaaagc gacggcggcc
     7081 gaggtggcga aatacatgtc gctggtgtcc tcgatcttcc tcaagctcat caagatgatc
     7141 atcgggccgc tcgtgctgtc gacgctcatc gtcggcatcg gccacatggg ggacgcggcc
     7201 accgtggggc gggtcggcgc caagaccatg gcctggttcg tctgcgcctc catcgtctcg
     7261 ctgctgctcg gcctcttcat ggtcaacctg ctgcagcccg gcgtcggcat cgccatcgcc
     7321 gaccagggcg ccgcggtggc caacctctcg acctccgcct tcagcatcga gaatttcgtc
     7381 gaccacatcg tgccgacgtc gatcttcaag tcgctggccg atggcgagat cctgcagatc
     7441 gtggtgttct cgatcttcgc gggcgtcgcc atcatggcga tgggcgagcg cggcaagccg
     7501 ctgatcgagc ttgccgacaa tatttcccac atgatgctcg tcatcaccgg ctatgtgatg
     7561 aagctcgccc cgattgcggt atgcgcctcg gtcgcttcgg tgataaccaa gtccggcccc
     7621 ggcatcctcc tcaatttcgc gaagttcctg ggcggcttct acctgacgct catcatgctc
     7681 tggctgatcc tggtcgtcgt cggctatctg acgctcggct cgcgcatggg caacctggtc
     7741 aagcggatgt acgagccctt cttgctcgcg ttctcgacgg cgagctcgga agcctcttat
     7801 ccgaagatcc tggaccagct cgagaaattc ggcgtctcca agaagatcgc gagctttgtg
     7861 ctgccgctgg gctattcctt caacctcgac ggcacgatgg cctattgcac cttcgcgacc
     7921 ctgttcatcg cgcaggccta ccatatcgac atgccgatct cgcagcaatt ggccatggcg
     7981 gccgtgctga tgctgacctc caagggcgtg gccggcgtgc cccgcgcctc gctggtggtg
     8041 atcctcgcca cgctcaagca attcggcatt ccggaggccg gcctcctgct catcctcggc
     8101 atcgaccagt ttctcgacat gggccgcagc gcgacgaacg tgatcggcaa ctcgctcgcg
     8161 gccagcgccg tcgcaaaatt cgaaaaggcg ctgggaccgg cccaggacga ggcgccggca
     8221 gcggcggacg gcgcgccgcg gccgaccgcc tcctgatcgg cgcgcgcttg ttgtatcctg
     8281 cgcgcggcga accgcttccg gcaccccccc tatcggcctc ctgccgcccg gaaccgggcg
     8341 gcggtgcggc atccgcgggg tcttgcgcct tgcggccgtc cgccagaagg ccgcctgggt
     8401 agcggccgcc ccgtgcctgg ccggcccgat atatccacgg gcaccatata gatattggct
     8461 atgtgtaaat aaggcctcac aacctgaggc ttctttctta taacgaaaaa tgcttagacc
     8521 gacctgcctt gctgcggtta cggttatgta aattctaagg ttgcattccc catgtatgtt
     8581 tatgacgaag tcgaccagca attggtcgac gagcgcgtgg ctcaattcgc cgaccagacg
     8641 cgccgctttc tcgatggcca gctgactgaa gacgacttcc gcgtgctgcg tctgcaaaac
     8701 ggcctgtacg tgcaaaagca cgcgcccatg ctgcgcatcg ccattcccta cggcatgctg
     8761 gccgcgcgcc agctgcgcac gctggcccat gtggcccgca cctgggaccg cggctacggc
     8821 cacttctcca cgcgccagaa cctgcagctg aactggccgc gcctggaaga cgtgcccgcc
     8881 atcctggccg agctggcccg cgtgcaaatg cacgccatcc agaccagcgg caactgcatc
     8941 cgcaacacca ccaccgacca cttcgccggc gtcgcgcccg atgaactggt gaacccgctg
     9001 gtgtggtgcg agatcgtgcg ccaatggtcg gccctgaacc ccgaattcgc cttcctgccg
     9061 cgcaagttca agatcgccgt gagcggcgcc atcgaagacc gcgccgccgt gggcgtgcac
     9121 gacctgggcc tgcaggccgt cgagcgcgac ggcaagctgg gctttcgcgt gtgggtgggc
     9181 ggcggcctgg gccgcacgcc catggtgagc cacttgctgc agcctttcat ggaatggcag
     9241 cacctgctga cctatctgca cgccgtgctg caggtctaca acctgcacgg ccgccgcgac
     9301 aacaaataca aggcgcgcat caagatcctg gtgaaggatc tcacgcccga cgtctttgcc
     9361 cagcaggtcg acgaagcctg gcaattgctc aagaacggcc ccgacaccgt cacgcaggaa
     9421 tacgtcgacg ccatcgccgc gcgcttcacg cagcccggct acgacgcgca ggccgccggc
     9481 gagcccgatg aaaccgaggc gcgcgccgcc gagaacccgt cgttcgcgca ctggctgcgc
     9541 cgcaacacgc atccgcacaa agtgcccggc tacgccgccg tgacgctgtc gctcaagccc
     9601 catggcgccc cgccgggcga catcaccgcc gaccagatgg acgccgtggc cgacctgtcc
     9661 gagcgctacg gcttcggcga actgcgcgtc tcgcacgaac aaaacctgat cctgcccgac
     9721 gtgcgccgct cgcagctcca cgccctgtgg caggaactgc gcgagctcaa gctggccacc
     9781 gccaacatcg ggctcctcac caacatcatc gcctgccccg ggggcgactt ctgcgcgctg
     9841 gccaacgcgg tgtccatccc cgtggcccag gccatccagg aacgcttcga caatctcgac
     9901 tacctgttcg agatcggcga gctggacctg aacatctcgg gctgcatcaa cgcctgcggc
     9961 caccatcacg tgggccacat cggcatcctg ggagtggata aggccggcga ggagtggtat
    10021 caggtcacga tcggcggccg gcagaacggc gccgcgcgcc cgctgcccga cctggaatcc
    10081 acgcgcggcg tgggcgcggc catcggccgc atcatcgggc cgtcgttcgc ccgcgccacc
    10141 gtgccgctgg tggtggactg tctgatccgc acctacctgg gcctgcgcga gagcgatgcc
    10201 gaacgcttca tcgacgtggt cgaccgcctc ggcatcgaac cgttcaagca cgatgtctat
    10261 gccgatccgg aagttatcaa ggccgccgaa caacgcgagg tggcccatgc ctgacgcccc
    10321 catcgccacc gcgccggcac ccagcctgat ccgcaacggc ggcatcgagg cagacgcctg
    10381 gcaggtcttc cagccaaccg agaacagcgc cctgcccgcc gacgaagccg gctggatcgt
    10441 gccgctgccg gcatggctgt cgtcgcccgc cgccttgcgc gcgcgccggc acccggtggg
    10501 cgtgctgctg ccctcggaca ccgaactcaa ggattgggcc ggcgaggcag gcaaacccgg
    10561 caagatcgac ttcacgggca tcgccttcat cgccgtcgat tttcccgtct ataccgacgg
    10621 gcgcggctac acgctggcgc agcaactgcg ccgccaatac ggctacgcgg gcgaactgcg
    10681 cgccgtgggc gatgtgctga tcgacaccat ccactaccag gcgcgggtgg gcttcgacag
    10741 cttcctggtc aagcccggcc acgacccggc caaggcgctg gcggcgctgc acacgttctc
    10801 ggtgcactac cagaaggcgt accgcgtgcc ggcgtagagc cggtcgcaag ggggtttaca
    10861 ccccttgcgc attcaacccg tagcggccgt ccgaggacga tttcagcgca ttgcgtatga
    10921 gttcggcgat ggcgtgctgc cgcggcgtcg gcatccggtc gatcagggaa gaaacgctga
    10981 tcgagaaccg cggatagccc ttgctgtcgc acagggccac gcccacgccc agcacgccag
    11041 gcaccgccga gttgcccacc acggcccagc cgcgtgcccg ggtgttatcc accaatttgt
    11101 gcatcttgcc ggcggtcatc ccaccgtagg cgtgcagcgc aagcccattg ctcgcgatca
    11161 cctcgccggc ctcggccggc gggagggccg ccagcagggc caaccccgca gaaccgacgc
    11221 ccagcggctg tcggtgcccc acggggactg acaacacctg cacgggatag ctgccaagct
    11281 cgcgatgcag gcacagcgca tcgtcgcccg cccggcatat caaaaaagac gaatcgccca
    11341 gctcgtcgct gatctggcgc agcacgggct gcaactggcg aatcgcctgg acatgcggat
    11401 cgatggcggg tgcgatctcg ctatcggcca ggcgatagcg cggtccgtcc tggtcgcgca
    11461 gcgcatagcc ctcttccacc agcacgtcga gataccggta gaccgtggaa cgctgcatgc
    11521 cggcggcgcg ggcgatttcc accacatgca ggctgtccat ccccgcctgg cgcaaaacgc
    11581 ccagcacgcg caagccccgg cgcaggacgc aaggaccgga atcggttgtc tccatcttgt
    11641 tcattttttg gacagcttga attggccgtg atgccaatac tgcggatcat aaagctggac
    11701 caccagtgtg ttgtccaaat attagacaaa aacaaatcag acaagaacga catggaggag
    11761 acaatgaaca aaccccgcac ccggatgctg ggcgcgctgg ccgtcgccgg cgcgctcgcc
    11821 tcgcccgccc cggcgcccgc cgccaatgcc tatccgcagc accccatcac catcgtcgtg
    11881 ggctacgccg ccggcggcgc caccgatatc gcggcgcggc tcatctccaa gccgctttcc
    11941 gaaatactgg ggcaatccgt cctggtcgag aacaagaccg gcgccaacag caatatcggc
    12001 gccgaagtcg tcacccgttc ccagccggac ggctacaccc tctacctcgg gtcgatagcc
    12061 aacaccatca accagacgct gtacgccaag ctcaactacg acttcgtcaa ggacttcaag
    12121 cccgtagggc tgctcgccac gatcccgaac atcctggtgg tcaaccccaa actgcccatc
    12181 aagacggtgc aggactacat cgcctacgcc aagtcccacc ccggcaaatt gagctgcgcc
    12241 tcgtcgggca cgggctcttc catccacctg tcttgcgagc ttttcaagat ggagacgggc
    12301 acgaacatcc tgcatgtgcc ttaccgcggc agcgggccgg cggtggtcga tctgctgggc
    12361 ggccaggtcg attccatgtt cgacaatctg ccgtcttccc tgcctcaggt gcaggccggc
    12421 aagctgcgcg ccatcggcgt cacgtcgccg caacgcgtgc ccaccgcgcc ggacgtgccg
    12481 accatcgccg aatcgggcct gcccggctac cacgtgcaat cgtggttcgg cctggtcgcg
    12541 ccggccggca ccccgcaagc cgtcattgaa cgcctgaacg ccgcactgaa caaggcgctg
    12601 gcgcagcccg gcgtgcaagc cctctacgcc caatccggct tttcgctgcc cgagtcgccc
    12661 aactctcccg aaaccttcgg ccacttgatt tcgaccgaaa tcgaccaatg gggcaaggtc
    12721 gtcaaaaccg ccggaatcac gaccgaataa accaaggact tctggatgta tcccatcgat
    12781 tttttctggc gggcggccga gcgccacccc gaccgcatcg cgctcgacgg tccggacgga
    12841 cgcctcacct atcaggcgct ggccagccgc gtcaacgcgc tggccgcggc gctgcagacg
    12901 ctcgatccgc agccccagac gagagtcgcg atctgcgccg gcaattcggc ccgccacctc
    12961 atcgcgctgc tggccacgct ggccagcggc aaggtctggg ttcccttgaa ctaccgcagc
    13021 acgccgcacg aaatcagccg catcatcgac gcgacggagc cttccatcgt catggtcgat
    13081 gcgtcgggcg aagcgctggt cccggccgcc ccgcatgcct acctgcgcct gcacgccggc
    13141 gatgcccacg gcctggatgc cctgatcgcc agccacgctg gcgatcagcc cgcgcgccac
    13201 gatctgcctc gcgacgcgac gcaagccatc aagttcactg gcggcacgac cggcatgccc
    13261 aaaggggtca tgcagcccta tcgggcatgg aacaccaaca tcatcaacca gatcgacgcc
    13321 tggcagctca ccccggaaga ccggtacgtc atggcggcgc cggtcaccca cggcaccggc
    13381 acctacctgc tgccggtact ggcccggggc ggcacgcacc tgctgctgga cggcacctct
    13441 cccgccacgg tcacccaggc tttccgggaa cggggcggca ccctgagctt catgccgccc
    13501 accctgatct acatgctgat ggcgcagccc ggcgtcaccc aagccgactt cccgcatctg
    13561 cgcaacctca tctacggcgg cgcacccatg cccgccgaca aggtcgacca ggtacgcgat
    13621 ttcttcgggc ccgtcctggc cacgagctac ggacagaccg aggcgccgca gctggtgacc
    13681 gtcatgcgtc cctgcgactt cgaggctccc gccaaccacg gctcggtcgg ttgcgtgacg
    13741 tggctctccg acatggccgt catgggcccc gacggacgac tcctgccacg cggccagatc
    13801 ggggaaatcg tggtgcgcgg cgaccttgtc atgaccggtt attggcgcct gcccgagaaa
    13861 accgccgaaa ccctggtgga cggctggctg cgcacgggcg acggcgggct gatcgacgaa
    13921 cggggctacc tgttcctgaa agaccggctg cgcgacgtga tcatcaccgg cggattcaac
    13981 gtctatcccg tcgacgtgga gaacgcgctg tcgacccacc ccgccgtgta cgaatgctcg
    14041 gtcttcggca tacccgacga gaagtggggc gaagccgtgc acgcggcggt gcagttccac
    14101 ccgggcgccg aggccacgga cgacgagttg aaagcgcatg tccgggcatt gctgggcccc
    14161 gtgcagaccc ccaagcacat ccacgtccac cggagcctgc cccgctcgtc agtgggcaaa
    14221 gtactcaaaa ccgcggtgcg cgacgccgcc ctgaaggaga caccatgacc acgcccgaaa
    14281 cccacctgtg cgcacaccac ctgaccggca tgtactaccg ggacgccgac ctggtcaatg
    14341 acctgatcgg cgagcagacc ttcgtccagg tgctcttctc ccaaatcatg ggccgcgagg
    14401 cccgaccggt cgacctgcgc atcgtcgacg ccgtgctcat caccctgatg gaacacggcc
    14461 tgacgcccag cgcgatcgcc acccgtctca tctatatgag cgcgcccgag aacctgcaag
    14521 gcgccgtcgc ggccggcctg atggccgtcg gcagccagtt cgtcggcacc atggaaaatt
    14581 gctcgcgcct attggaccgc atcaaccagg ccgccgatcc gagggccgag gcgcaggcga
    14641 tcgccggcga ataccgcgcg gacaagcgcg cgctgcccgg tttcgggcac cacctgcaca
    14701 agcccgacga tccgcgcgcc gtgaaactgc tgtccctggc cgaagccgaa cccgacctgg
    14761 accagaccca cgtccacgcc ctgcggctgc tggccggcgc cgtcgacgcc gcccacggca
    14821 ggcacatcac catcaatgcc accggggcgg tcgccgcgct gctcggtggc atcggcgtgc
    14881 cgaccgccct gatgcgaggc ttcgccgtca tctcgcgcgc cgccggcctg gtctcgcacg
    14941 tggccgaaga gcagcaaagc ccgtcgggcc gcttcatctg ggaaacgata gaccacgcca
    15001 tcccctacgt aggcgcgggg aaatcccatc agccggggaa acagccatga agccccacgc
    15061 gctcgtgacc ggcggcagcc agggcatagg ccgcgccatc gtgcaacgcc tgatccatga
    15121 cggcatgcat gtcctcaaca tcgaccggtg cgtgcccgaa gcgccgctgc agggcgaaac
    15181 ccatgtgcgg ctcgatctca ccgacgtggg cgccgtgcga gaagccattg ccggcatcgc
    15241 tcgcggcacc ccaatactga ggctggtgaa caacgccggc atcgtgcacc cggcgcccct
    15301 ggaagacgcg agcccggaag acctggacgc cgtcgtcgcg ctcaacctgc gggcgcccat
    15361 gctgctcatg caggggctgc tgcccgtcat gcgcgcgcac ggcttcggac gcgtggtcaa
    15421 catcgcgagc cgggcggccc tgggcaagag cgaacgaacc gtctacgccg cgaccaaggc
    15481 cggcctgctc ggcatgacgc gcacctgggc gctggagtgc ggggcccacg gcatcaccgt
    15541 caacgcggtg ggccccggcc ccatcgccag cgccctgttc gaccgcgtca acccgccggg
    15601 cgcgccccag accgaacgca tcatcgccac catccccctg cgccgcatgg gcaggcccga
    15661 cgaggtggcg catgccgtcg cctcgctgct ggacgagcgg gccggcttca tcaccgggca
    15721 ggtcctgtat gtctgcggcg gcatgacggt cgggctgggg aacgcggcat gaccacgcgg
    15781 caggacgacc ggctctcggg caaggtggcc ttcgtcgcgg gcgcgggctc atcggccgca
    15841 ggctggagca taggcaaggc gagctgcgtc acgctggctc gccgcggggc catggtggtg
    15901 gcgctggatt gcgacctggc cgccgcccag gacgccgccc acgaagtcga gtcggccggc
    15961 ggccgggcgc tgcccgtcca ggccgacatc gccgacccgc atgccatgcg ggccgccgtg
    16021 gatgccgcgc tgcgccattt cggccgcatc gacatcctgc aggccaacgc gggcatcggc
    16081 aaagtgggcg gccccgagga catttccctg gaagactgga accgcatcca gcaggtcaat
    16141 gtcaccagcc tgctgatcgc catgaacctg ttcgccccca tcatgcgccg gcagggtggc
    16201 ggcgccgtgg tcgcggtgtc gtcggtggcg ggcatacgct ataccgggta cccgcacctg
    16261 gcctacagcg tgagcaaggc cgccgtcatc cacttcgcgc gcatggcggc gcagcagtac
    16321 gcggccgacg gcatccgcgt gaacacggtg atcccgggac tgatcgacac gccccgcgtc
    16381 gcccgcaacg tcgcccgtat gttcgacccg gacgacctgc aggcggcgcg cacggcgcgt
    16441 gacaggcagg tcccgatggg ccgcatgggc acgccgtggg aggtggccga cgccgtggcg
    16501 ttcctggcct cggacgaggc ctcatacatc accggaacgg aattgctggt ggacggcggg
    16561 ctgacgggca aatatgcctg aggcgccatg cgggccggac cttggccaaa tgacgccgat
    16621 gcgcaagcct ggaaactccg cagggttact gcgaacccgg ttcgccggcc gacatcttgc
    16681 gcaggatgaa gcgctggacc ttgccgctgg gcgtcttcgg caagccttcc acgaactcga
    16741 ccagccgcgg ataagcgtgc gccgagagcc gtttcttgac gtaattgctc aattcctcgg
    16801 caagcgccgg gctggcctcg accccttgcg acaacaccac gtaggccttg acgatctcgg
    16861 tgcgctccgg gtccggcacg ccgacgacgg ccgcctccat gacggcgggg tgttcgatca
    16921 gcgcgctctc cacgtcgaag ggaccgatgc ggtagcccgc cgaagtaatc acgtcgtcgg
    16981 atcggccgat gaagctcacg gagccgtccg gctcgaactc cacggtatct cccgtccggt
    17041 aatagccgcc gtcgatggcg ggcgtgtcct gctgccaata ccccgtaaac cacaacatgg
    17101 gtgaacgcgc gatgtcgatg gacaacacgc cgg
//
LOCUS       DASWPO010000190        15475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2721670, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000190 DASWPO010000000
VERSION     DASWPO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15475
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721670"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..532
                     /locus_tag="VGC69_09990"
     CDS             2..532
                     /locus_tag="VGC69_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0295649.1"
                     /translation="MLFAITGLTLNNAERIESKPRVTVRQAALPADVLAAANQAAASK
                     PATLPAPLAGWLRREWRIDTDDHKPEWSPDEVYVSLPRPGGDAWTSVDLKTGQARYEV
                     TDRGWIAYLNDLHKGRNTGRAWSWFIDFFSIACLVFALTGLLLLKMHASKRAATWPMV
                     GLGVLIPLLLALAFIH"
     gene            573..1088
                     /locus_tag="VGC69_09995"
     CDS             573..1088
                     /locus_tag="VGC69_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEY0295650.1"
                     /translation="MRQHLLPLLFVGAIARSAHAADLNVNVELPRLDTAEYHRPYVAI
                     WIESADQSTARDLAVWYDVAKRNNAGTQWLKDLRLWWRRSGRDQQMPVDGVSGATRPA
                     GKHELSFAGAAKPLADLKPGSYAVVVEAARESGGHELVRVPFDWPPKQASQPAAQGHA
                     ELGAIALDIKP"
     gene            1158..1970
                     /locus_tag="VGC69_10000"
     CDS             1158..1970
                     /locus_tag="VGC69_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEY0295651.1"
                     /translation="MKKPARLAATLAAALALSLPLYAHARSPWLMPSVTVLSGNGAWV
                     TFDGSMGDDTFYPDHALHLDGLAIQAPDGSAAQAENGSEGKLRSTFDVNVRQPGTYRI
                     AIADDTVFARWQENGQRKRYFGKADGLAGAVPAKAEDLHISQNLNRAETFVTLGKPSM
                     PRDDVKQGLSLVPVTHPNDLYAGDTATFRFMLDGKPAANLDVEVIASGSRYRDQPGGT
                     PLKTGADGSFKVTWPAPGMYLVHAQAEDKSTSVPRAEMRRMSYGATLEVLPQ"
     gene            1967..2914
                     /locus_tag="VGC69_10005"
     CDS             1967..2914
                     /locus_tag="VGC69_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY0295652.1"
                     /translation="MTQAAALAGATMGTTWSARLNRPAGVNDAQAQDAIQHALDAVVA
                     QMSHWEQGSDLSRYNRAAPGWHDIPAGFGDVLGCALALAAGTGGAYDPTLGPLVDAWG
                     FGPRGRAIEPPSASALQALRERCGWQKVRLDATARRAWQPGGLSLDFSSIAKGYGVDQ
                     AAFALDALGIPDYLVEVGGELRSRGQRPDGLPWRVAVETPDASGSQALAIRLPDCAIA
                     TSGDYRRYIESADGRYSHTLDPRTGRPVDNGLASVTVVCGNGTGSCMLADALATALTV
                     LGPQDGMAYAQTHKLAALFIVRESAGHAVLVTPAFHALL"
     gene            3023..4174
                     /locus_tag="VGC69_10010"
     CDS             3023..4174
                     /locus_tag="VGC69_10010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0295653.1"
                     /translation="MNAMQQLEPIVRDVIAPSAAETDQQASYPRAALNALGQAGLLGL
                     ISAREVGGMGAGLPEAVQVIERIAQDCPCTAMVLTMHYCGVSLIEPFADETVRRDIAQ
                     GAHVTTLAVSEAASRSHIWAPAGTASAGDPADADGDVRLNALKSMITSAGEADSYVWI
                     SRATQGEGNTLWLVDSRLPGLRIPARFDGMGLRGNASSPIEAVDVHVPRRCMLGADGE
                     GELNKARYLMPFFPTLIATTSIGLMQGILRRAVEHVASTRFSDTGASLGDLPTIRAYL
                     ARAWLQAEQARLLRDATVASVGRADPDTRIRLLQVKAAAAEAALAVTETAMRVCGGAA
                     FRRETGIERLFRDARAASVMAPTTDVLYDTLGKALCEGAAIAPAAAQAA"
     gene            4213..5073
                     /locus_tag="VGC69_10015"
     CDS             4213..5073
                     /locus_tag="VGC69_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY0295654.1"
                     /translation="MIASPLRLGAVAYAPKVITIWEGFKAHFAARGLYFDYVLYSNYE
                     ALTESLLAGDVQLAWNSPLAFVRADRMARARGQRVQSIAMRDTDLDLHSLLVVPADSP
                     VHSLADLRGKTLGFGAVDSPQATLIPLDHLRQNGLAGGADYTVRRFDVLGGKHGDHIG
                     GERDAARAMVAGDIAASWMVGKNYQLFAKEGTLPAGGTRVLATTGAFDHCNMTSGPAL
                     DPKAADAFRDILLAMSWDDPQVRPLLELEGLKVWQPGRDTGYALLERAVTDENFYDAA
                     GHITADGYRY"
     gene            5080..6294
                     /locus_tag="VGC69_10020"
     CDS             5080..6294
                     /locus_tag="VGC69_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0295655.1"
                     /translation="MHTTPLATVDLEHLGFDAGAHLLVKQALAQVEAGQHIAVRGQAP
                     GWQAQLAAWCQAQGHAVLPGVADSNGAALVRKGSAQAGRWRGAAQTGHSDPDIEQAIA
                     EQARPCWGLAARGAAVEAGSPDFDFRLADKAQMWTDNAADLYAQAAAAQWNADRDIDW
                     NNAPELPDAVEDAIVQIMTYMVENENAALLVPARFLGQLHPHYRELQAALAIQVADEA
                     RHIDAFTRRIRRHGHEPALSTAGGQASLKSLLDENDFSIAGFLLSVLGEGTFVNLLQF
                     LQAEAPDPLTRQIARLAARDEARHVALGMSHLLYRLQREPDFRLQLARAVEQRHDALE
                     GTAGLNEEVFDALIIVAAGDLTPAALARGAQRVQQLMRDMAGGRYAKLVRLGFGREEA
                     EQLSALHTRNFM"
     gene            6359..7495
                     /locus_tag="VGC69_10025"
     CDS             6359..7495
                     /locus_tag="VGC69_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEY0295656.1"
                     /translation="MQASPLPVLPPVARPGGPLSQVDTPSLVLDLDAFEANLRAMQDW
                     ADQHGVALRPHAKAHKCPQVTLRQLALGAKGICCQKVSEALPFLAAGVRDIHISNEVV
                     GASKLALLAELAGHADASVCVDNLSNLQDIAAAVARAGTRLTVLIEVDIGAHRCGLTD
                     AAELLALARAIATAPGLRFGGLQAYHGSSQHVRSRAERIATCKAGAGKAAAFARLLRD
                     NGIACERITGAGTGSAEFDAASGVYTELQAGSYAFMDVDYGANQWEGASLTFRNSLFL
                     WSTVMSRPTAQRAVVDIGLKTTSTESGLPAVHGRTGLRCVGVSDEHGTLEILDGADAP
                     ALGEKLRLVPSHVDPTFNLHDTLVTYRGDTVAELWPIAARGQSR"
     gene            7659..8441
                     /gene="xth"
                     /locus_tag="VGC69_10030"
     CDS             7659..8441
                     /gene="xth"
                     /locus_tag="VGC69_10030"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0295657.1"
                     /translation="MKLATWNVNSLNVRLPQVLDWLAANPVDALCLQELKLADDKFPL
                     QAFTDIGYHAAWAGQKTYNGVAVLSRQPGATMVRNIPGFDDPQQRVVAVTLPSPAGDV
                     RVISAYCPNGQALDSDKYPYKLAWFAALHRWLQEEMAAHPRLAVLGDYNVAPADEDVH
                     NPEKWVGQVLVSEPERAAFRTLLDLGLTDAFRLFEQPPKSFSWWDYRQFGFKRNAGLR
                     IDHILLSDPLKAACIECVIDKGPRGNEQPSDHAPVIATLKLD"
     gene            8450..9475
                     /locus_tag="VGC69_10035"
     CDS             8450..9475
                     /locus_tag="VGC69_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0295658.1"
                     /translation="MPTHEPQDGIYQAGNVVLQSGRTFRNMFISYRTFGTLNADKSNA
                     ILYPTSFSAQHTDVEHMVRVDGGLDPGKYFIIIANQFGNGLSSSPSNTPWPDIGTRYP
                     DVTYYDAARVQKQMLEELWGIEKLALVYGWSMGAMQAYHWAAIFPDAVERIAVVCGSA
                     RCAPHNQVFLEGVRHALIADPAYRDGVFTEKPVRGLRAMGRVYAGWAMSQTFYRQELW
                     RTFGCSSLEDYLVSQWEVTYARRDPCDLLAHLWTWQHGDLSANELYHGDLPRALAAIK
                     ARVLLMPGDHDLYFQVADNELELPHLKHGQLKPIPSIWGHRAGNPMNQPEDRAFIDNN
                     VKALLAS"
     gene            9509..10435
                     /locus_tag="VGC69_10040"
     CDS             9509..10435
                     /locus_tag="VGC69_10040"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0295659.1"
                     /translation="MEKNAPFRLKAQDVMPLSAKSDLPGLLRASGHLGAIVLADMALW
                     HALGTWWAVPLTFLSGYVVAFLFCVEHETAHQTAFRTRALNYVFGHLASLAILYPYEY
                     YRAFHWDHHRYTQDPARDPELAVPQPASLAGIAWACVGMAALTLRVRKLLEHAAGRAN
                     EPWVPDNKRALVVLEARCYLLVYALVAVASIATGSLAALWLWLLPLLAGQCLMRPYLM
                     SEHVGCLHDPDTLANTRTTYTNAVVRFFAWNMPYHAEHHTYPSVPFHALPKLNVLLAG
                     HIANTEQGYIAAFSAVLRHLTRRGEADAQAKA"
     gene            10489..10564
                     /locus_tag="VGC69_10045"
     tRNA            10489..10564
                     /locus_tag="VGC69_10045"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10522..10524,aa:Ala,seq:cgc)
     gene            complement(10951..12342)
                     /locus_tag="VGC69_10050"
     CDS             complement(10951..12342)
                     /locus_tag="VGC69_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0295660.1"
                     /translation="MPTDIDASPTARLAQWCSRPDHNWNRDCLDGARRAFLDTTAVML
                     AGSGEVAVRRTAAAALSWGSGAAHVVGQAAMLAAPWAALINGTAAHALDYDDVLDPAR
                     SHPSAALVPALYALAEETGASGHDCLEAYLVGFEVMTRLGEAMNLDHYGRGWHTTLSL
                     GAPGVAAACARLLRLDTRATAMAISLATSMAGGSKCQFGTMAKPLHAGLAAKNGLVAA
                     RLAQAGVSAIAEPFEGTWGYREMTSGAGSPGFAGALTNLDGEPAMTQYGIWAKPYPCC
                     AGAHRAIDAALALAHAYPDVMGRAVRITASVSVIASRNLKYRVPADVSEARFSLPYCV
                     CTALVDGAPGPASFTAEAITRPFVLALIERFETIVDTEVGSGDVSASRESSTVTLYLN
                     DGRTLSESVDIPHGYPPRMLGEADLSRKFFACVDNVLPMTAAHRLHEQLLDIEALPRA
                     AALAAMLGEARPA"
     gene            complement(12401..13156)
                     /locus_tag="VGC69_10055"
     CDS             complement(12401..13156)
                     /locus_tag="VGC69_10055"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295661.1"
                     /translation="MIVLLAFLVAPILVVVYVSFTSSSFISLPVESFSLRWYAQIVRY
                     RPFLDSLLLSIELALASTACGAVLAVPAALALGRMRGRITSGIVVVLLAPISIPAVVL
                     GFAMLYYLSALSLGTSFLSLLIAHTVIAIPYISRTVLSVYRGIGPDLEESAAILGAGK
                     WETLRRVTLPLVLPGIFAGSVFALLISLDNLPISFFFGTAETNTLPVVMLSYMQNQFD
                     PSIAAIATVQTLISLVALFIVNKLYGLSSLMAA"
     gene            complement(13228..14154)
                     /locus_tag="VGC69_10060"
     CDS             complement(13228..14154)
                     /locus_tag="VGC69_10060"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295662.1"
                     /translation="MSRAQGPAADGAVGPARERAGRAAGAPAPRASYRLMRPAWLLPA
                     LAFLLIFFIGPLLYNVLINGSAAMGARHDSWFYYHKLFTDPYYLGTLAETLKLSLVVT
                     LGCLVFGYPASYYMVRHAGRWNGVIVFLLIAPILTSVIMRTFGWQILLGRHGPVSALL
                     VAFGLFTRPVDLARMPFSVYLSLVHVMLPFMILSITAVLKGVDRRCEEAARVLGAGWW
                     QAFFLVTFPLSMEGVASGCVLVFVVTNGSFLANLLLGDGEVNTLPLQIYQQFNLTHDI
                     AFASAMGNVLLVAALVCLIVQIRLLRRKGVNA"
     gene            complement(14151..15245)
                     /locus_tag="VGC69_10065"
     CDS             complement(14151..15245)
                     /locus_tag="VGC69_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295663.1"
                     /translation="MKQDASSLVLCSISKRFGANQALHPLDLQVEEGELLALLGPSGC
                     GKTTTLRIVAGFESSDTGSVRIGERDITALPPNQRGLGMMSQSYSLFPHMTVAENIGF
                     GLRMRKVPAGEATKRLIEALQLVRLQGFEDRYIHQLSGGQQQRVALARAIVTQPSVLL
                     LDEPMGALDKHLRERMQFELRSLQQRLGITSVMVTHDQEEALTMSDRIAVMHEGRIVQ
                     AGTPAEVYARPRTRFVAEFLGTANIFDATVDEAGANGQGRVRLDIAPERVLPVGGLKQ
                     ARVGQRVRVAVRPERFTFASGEAAAFGAQISAVIFRGNHYAYELRAPGRGAPVFITSQ
                     DRVAAAPGQSVGLTWPAEDAVVLVDEDPPR"
     gene            complement(15267..>15475)
                     /locus_tag="VGC69_10070"
     CDS             complement(15267..>15475)
                     /locus_tag="VGC69_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295664.1"
                     /translation="FIDFVLSPEPEACWGNALQYGMSNTQTQFSPKVAPRITKLPEII
                     WPPFDQLDAHQGAWVEQWHKTVGN"
BASE COUNT         2739 a         5620 c         4801 g         2315 t
ORIGIN      
        1 gctgctcttc gccatcaccg gcctgacgct gaacaacgcc gagcgcatcg aatccaagcc
       61 gcgcgtcacc gtgcgccagg cggcgctgcc cgccgacgtg ctggcggccg ccaaccaggc
      121 cgccgcgtcc aagcccgcca ccctgcccgc cccgctggcc ggctggctgc gccgcgaatg
      181 gcgcatcgac accgacgacc acaagccaga atggtccccg gacgaggtct atgtttccct
      241 gccccgcccc ggcggcgacg cctggacctc tgtcgacctg aaaaccggcc aggcccgcta
      301 cgaagtcacc gaccgcggct ggatcgccta tctcaacgac ctgcacaaag gccgcaatac
      361 cgggcgcgcc tggagctggt tcatcgactt cttctccatt gcctgcctgg tgttcgcgct
      421 gaccggcctg ctgctgctca agatgcacgc ctccaagcgc gccgccacct ggcccatggt
      481 gggcctgggc gtactcattc cactgctcct ggccctggcc ttcatccact gaccgcttcc
      541 actacttagg acgagacacc gtgcaccctg ccctgcgtca acacctgctg cccctgctat
      601 tcgtcggcgc catcgcccgc agcgcccacg ccgccgacct gaacgtgaat gtcgaactcc
      661 cgcgcctgga caccgccgaa taccaccggc cctacgtggc catctggatc gagagcgccg
      721 accagagcac ggcccgcgac ctcgccgtct ggtacgacgt ggccaagcgc aacaacgcag
      781 gcacgcaatg gctgaaggac ctgcggctat ggtggcgccg cagcggccgc gaccagcaga
      841 tgccggtgga cggcgtcagc ggcgccaccc gcccggccgg caaacacgag ctgtccttcg
      901 ccggggccgc caagcccctg gccgacctga agcccggctc ctatgccgtg gtggtcgaag
      961 cggcccgcga gtccggcggc cacgaactgg tgcgcgtccc cttcgactgg ccgcccaagc
     1021 aggccagcca gcccgccgcc caagggcatg ccgaactggg cgccatcgcg ctggacatca
     1081 agccctgacc cgccccggca cccgttaggc cccgatcgca tggcgcgccg ccgcgcgccc
     1141 ctaggagaac cccgcccatg aaaaaacccg cccgcctggc cgcgacgctc gccgccgcgc
     1201 tcgccctgag cctgcccctg tacgcccatg cccgcagccc ctggctgatg ccctccgtca
     1261 ccgtcctctc aggcaacggc gcctgggtca ccttcgacgg gtcgatgggc gacgacacct
     1321 tctatcccga ccacgccctg cacctggacg gcctggccat ccaggccccc gacggcagcg
     1381 ccgcgcaagc cgagaacggc agcgagggca agctgcgcag caccttcgac gtgaacgtcc
     1441 gtcaacccgg cacctaccgc atcgccatcg ccgacgacac cgtcttcgcc cgctggcagg
     1501 agaacggcca gcgcaagcgc tacttcggca aggccgacgg cctcgccggc gccgtccccg
     1561 ccaaggcaga agacctgcac atctcgcaga acctgaaccg cgcggaaacc ttcgtcaccc
     1621 tgggcaagcc cagcatgccg cgcgacgacg tcaagcaggg cctgtccctg gtgccggtca
     1681 cgcatcccaa cgacctctat gccggcgaca ccgccacctt ccgcttcatg ctcgacggca
     1741 agcccgccgc caacctggac gtggaagtca tcgccagcgg cagccgctac cgcgaccagc
     1801 ccggcgggac cccgctcaag accggcgcgg acggcagctt caaggtcacc tggccggcgc
     1861 ccggcatgta cctggtgcac gcccaggccg aggacaagtc caccagcgtc ccgcgcgcgg
     1921 aaatgcgccg catgagctac ggcgccaccc tggaagtcct gcctcaatga cccaggccgc
     1981 cgcgctggcc ggcgccacca tgggcaccac ctggtcggcg cggctgaacc ggccggccgg
     2041 cgtgaacgac gcccaggcgc aagacgccat ccagcacgcg ctcgatgccg tcgtggcgca
     2101 gatgagccat tgggagcaag gctccgacct cagccgctac aaccgcgcgg cgcccggctg
     2161 gcacgacatc ccggccgggt tcggcgacgt gctgggctgc gcgctggccc tggcggccgg
     2221 caccggcggc gcctacgacc ccaccctcgg cccgctggtc gatgcatggg gtttcggccc
     2281 gcgcggccgc gcgatcgaac cgccctcggc cagcgccttg caggccttgc gcgaacgctg
     2341 cggctggcaa aaagtgcggc tcgacgcgac ggcgcggcgc gcctggcagc ctggcggcct
     2401 ctcgctcgat ttctcgtcca tcgccaaggg ctacggcgtc gaccaggccg cgttcgcgct
     2461 cgatgcgctg ggcattccgg attacctggt ggaagtgggc ggcgagctgc gctcgcgcgg
     2521 ccagcggccc gacggcctgc cctggcgcgt ggccgtggaa acgcccgatg ccagcggcag
     2581 ccaggcgctg gccatccgcc tgccggactg cgccatcgcc acctcgggcg actaccggcg
     2641 ctacatcgag agcgccgacg gccgctattc ccataccctg gacccgcgca ccggccgccc
     2701 cgtggacaac ggcctggcct cggtcacggt cgtctgcggc aacggcacgg gcagctgcat
     2761 gctggccgat gcgctggcca ccgcgctcac cgtgcttggc ccgcaggacg gcatggccta
     2821 cgcgcagacg cacaagctgg ccgccttgtt catcgtgcgc gaaagcgcgg ggcatgccgt
     2881 gctggtaacg ccggcctttc acgccctgct gtagcgcctg ccaggcctta agacgactgc
     2941 ccgtctcctt gccgcccgca ccgcgccgtg gccggcgcta tcatcgtgcg aggcgactca
     3001 gcgataggga gcaaatgcgg tcatgaatgc catgcaacaa ctcgagccca tcgtgcgcga
     3061 tgtcatcgcg ccatcggcgg ccgaaacgga ccagcaggca agctatccgc gtgccgcgtt
     3121 gaatgcgctg ggacaagccg gattgctggg cctgatcagc gcgcgcgagg tcggcgggat
     3181 gggcgcgggg ctgcccgaag ccgtgcaggt catcgagcgc atcgcccagg actgcccctg
     3241 taccgccatg gtgctgacca tgcactattg cggcgtatcg ctcatcgaac cctttgccga
     3301 cgaaaccgtc cggcgcgaca tcgcccaagg cgcccacgtc accacgctgg ccgtttccga
     3361 agccgcctcg cgcagccata tctgggcgcc ggccggcacg gccagcgccg gcgacccggc
     3421 cgatgcggac ggcgacgtgc gcctgaacgc cctcaagagc atgatcacct cggccggcga
     3481 ggccgattcc tatgtctgga tctcccgcgc cacgcagggc gaaggcaaca cgctctggct
     3541 ggtcgacagc cggctgccgg gcctgcgcat tcccgcgcgc ttcgacggca tgggcctgcg
     3601 cggcaacgcc tcctcgccca tcgaggccgt cgacgtgcac gtgccccggc gctgcatgct
     3661 gggcgcggac ggcgaagggg aactgaacaa ggcgcgctac ctgatgccct tcttccccac
     3721 cctgatcgcc acgacgtcca tcggcctgat gcaaggcatc ctgcgccgcg cggtcgaaca
     3781 tgtcgcgtcg acccggttct ccgacaccgg cgcgtccctg ggcgacctgc cgaccatccg
     3841 cgcctacctg gcgcgcgcct ggctccaggc cgagcaggcg cggctgctgc gcgacgctac
     3901 cgtggcgtcc gtgggccggg ccgaccccga tacgcgcatc cggctgctgc aggtcaaggc
     3961 cgccgcggcc gaggcggccc tggccgtcac cgagaccgcc atgcgggtgt gcggcggcgc
     4021 cgccttccgc cgggagaccg gcatcgagcg cctgttccgc gacgcgcgcg ccgccagcgt
     4081 gatggccccc accaccgacg tgctctacga cacgctgggc aaggccctgt gcgaaggcgc
     4141 ggcgatcgcg cccgctgccg cgcaggccgc ctgacctttt ccctttccct tccgccgaca
     4201 aggaaacccg acatgatcgc cagccccctg cgcctgggcg ccgttgccta cgcccccaag
     4261 gtcatcacca tctgggaagg cttcaaggcc cacttcgccg cccgcggcct gtatttcgac
     4321 tatgtgctct attccaacta cgaagccctg accgagtcgc tgctggccgg cgacgtccag
     4381 ttggcctgga actcgcccct ggccttcgtg cgggccgacc gcatggcccg cgcgcgcggg
     4441 cagcgcgtgc aatccatcgc catgcgcgac accgacctcg acctgcactc gctcctggtg
     4501 gtgcccgccg acagccccgt gcacagcctg gcggacctgc gcggcaagac cttgggcttc
     4561 ggcgccgtcg actcgccgca agccaccctg atcccgctcg accacctgcg ccagaacggc
     4621 ctggcgggcg gcgccgacta caccgtgcgg cgcttcgacg tgctcggcgg caagcacggc
     4681 gaccacatcg gcggcgagcg cgacgcggcc cgcgccatgg tcgccggcga catcgcggct
     4741 tcctggatgg tgggcaagaa ctaccagctc ttcgccaagg aaggcaccct gccggccggc
     4801 ggcacacgcg tcctggccac caccggcgcc ttcgaccatt gcaacatgac cagcggcccc
     4861 gcgctggacc ccaaggcggc cgacgccttc cgcgacatcc tgctggccat gtcctgggac
     4921 gacccgcaag tgcgccccct gctggagctg gaaggactga aggtctggca acccggacgc
     4981 gacacgggct atgcgctgct cgaacgcgcc gtcacggacg aaaatttcta cgacgccgcg
     5041 ggccacatca cggccgacgg ctaccgctac tgaaccgcca tgcacaccac gcccctggcc
     5101 accgtcgacc tggaacacct gggcttcgat gccggtgccc acctgttggt caagcaggcg
     5161 ctggcccagg tggaagccgg ccagcacatc gccgtgcgcg ggcaggcccc cggctggcag
     5221 gcgcaattgg ccgcctggtg ccaggcccag ggacatgccg tgctgcccgg cgtggccgat
     5281 tcgaacggcg ccgcgctggt gcgcaagggc tcggcccagg ccggacgctg gcgcggcgcg
     5341 gcacagaccg gccacagcga tccggacatc gaacaggcca tcgccgagca ggcccggcct
     5401 tgctggggcc tggctgcccg gggcgcggct gtcgaggccg gctcgcccga tttcgatttc
     5461 cgcctggcgg acaaggcgca gatgtggacg gacaacgccg ccgatctcta tgcccaggcc
     5521 gcggccgccc agtggaatgc cgatcgcgac atcgactgga acaatgcccc cgaactgccc
     5581 gacgccgtcg aggacgccat cgtgcagatc atgacctata tggtggaaaa cgaaaacgcc
     5641 gccctgctcg tgcccgcgcg cttcctgggc caattgcatc cgcactaccg cgagctgcag
     5701 gccgcgctcg ccatccaggt ggccgacgag gcccgccaca tcgacgcctt cacgcgccgc
     5761 atccgccgcc acggccacga gcccgccttg tcgaccgccg gcggccaggc gtcgctcaag
     5821 agcctgctcg acgagaacga cttttccata gcgggattcc tgctgtcggt gctgggcgaa
     5881 ggcaccttcg tgaacctgct gcaattcctg caggccgaag cgcccgaccc gctcacccgc
     5941 cagatcgccc gcctggccgc gcgcgacgaa gcgcgccacg tggcgctggg catgtcgcac
     6001 ctgctgtacc gcctgcagcg cgaaccggac ttccgcctgc agctggcccg cgcggtcgag
     6061 cagcgccacg acgccctgga agggacggcc ggcctgaacg aggaagtgtt cgacgccctg
     6121 atcatcgtgg cggcgggcga cctgacgccg gccgcgcttg cccgcggcgc gcagcgcgtg
     6181 cagcagctga tgcgcgacat ggccggcggc cgctatgcca agctggtccg cctgggcttc
     6241 gggcgcgaag aagccgagca gctgtcggcg ctgcacacgc gcaacttcat gtagcgcggc
     6301 agcggcaaag ccgctttggc aatatgcttg cggggctaca ggaaaatcac ccatgcccat
     6361 gcaagcctcc cctctccccg ttctgccgcc cgtggcgcgc cccggcggcc cgctatcgca
     6421 agtcgataca cccagcctgg tgctggatct cgatgccttc gaggccaatc tgcgcgccat
     6481 gcaagactgg gccgaccagc acggcgtggc cctgcgccct cacgccaagg cgcacaaatg
     6541 cccgcaagtc acgctgcgcc agcttgcact gggcgccaag ggcatttgct gccagaaggt
     6601 aagcgaagcc ctgcctttcc tggcagccgg cgtgcgcgac atccacatca gcaatgaagt
     6661 ggtcggtgcc agcaagctcg cattgctggc cgaactcgcc ggccacgccg acgcgagcgt
     6721 gtgcgtggac aacctgagca acctgcaaga catcgccgcg gccgtcgcgc gcgccggcac
     6781 gcggctgacc gtgctgatcg aggtcgacat cggcgcgcat cgctgcgggc tcacggatgc
     6841 cgccgagctg ctcgcgctgg cgcgcgccat cgcaacggcg ccgggcctgc gcttcggcgg
     6901 cctgcaggcc taccacggct catcgcagca cgtgcgctcg cgagccgagc gcatcgccac
     6961 gtgcaaggcg ggcgccggca aagcggcggc ctttgccagg ctattgcgcg acaacggcat
     7021 cgcctgcgag cgcatcaccg gcgccggcac gggcagcgcc gagttcgacg cggcaagcgg
     7081 cgtctacacc gagttgcaag ccggctccta cgccttcatg gacgtcgact acggcgccaa
     7141 tcaatgggaa ggcgcctccc tgaccttccg caacagcctc ttcctgtggt ccaccgtcat
     7201 gagccgcccc accgcgcaac gcgcggtggt cgacatcggc ttgaaaacca ccagcaccga
     7261 gtcgggcctg ccggccgtgc acggccggac cggcctgcgt tgcgtcggcg tcagcgatga
     7321 gcacggcacc ctcgagatac tcgacggcgc cgacgccccc gccttgggcg aaaagctcag
     7381 gctcgtcccc tcccacgtcg accccacctt caacctgcac gacacgctgg tcacttaccg
     7441 gggcgatacc gtggctgaac tctggcccat cgccgcccgg ggacaaagcc gctgaatggc
     7501 caggagggga aatccctagc cccatgctcc aggcctcggc tccacgaacc ggaaacgcat
     7561 tcgattcatg ccggtccgtt cttgccggtc cgatgagcga atagaattgg cagtcgccgc
     7621 ttttgcgcag caagaagaac aaaccacgtg tccatcccat gaaactcgcc acctggaacg
     7681 tcaactccct caacgtgcgc ctgccccagg tcctggactg gctggccgcc aaccccgtcg
     7741 acgcgctctg cctgcaggag ctcaagctcg ccgacgataa atttcccctg caggccttca
     7801 ccgacatcgg ctaccacgcc gcctgggccg ggcagaagac ctacaacggc gtggccgtgc
     7861 tgtcgcgcca gcctggcgca accatggtgc gcaacatccc cggcttcgac gacccgcagc
     7921 agcgcgtcgt cgccgtcacc ctgcccagcc cagcgggcga cgtgcgcgtg atcagcgcct
     7981 actgccccaa cggccaggcg ctggattcgg acaaataccc ctacaagctg gcatggttcg
     8041 ccgcgctgca tcggtggctg caagaagaga tggccgccca cccgcgcctg gccgtcctcg
     8101 gcgactacaa cgtcgccccg gccgacgagg acgtgcacaa ccccgagaaa tgggtgggcc
     8161 aggtgctggt gtccgagccc gaacgcgccg ccttccgcac cctgctcgac ctgggcctga
     8221 ccgacgcctt ccgcctcttc gaacagccgc ccaagtcctt cagctggtgg gactaccggc
     8281 agttcggctt caagcgcaac gccggcctgc gcatcgacca catcctgctc tccgacccgc
     8341 tcaaggcggc ctgcatcgaa tgcgtcatcg acaagggccc gcgcggcaac gagcagccct
     8401 ccgaccacgc ccccgtcatc gccacgctca aactcgactg aggaacccca tgcctacgca
     8461 tgaaccccaa gacggcatct accaggcagg caatgtcgtc ctgcaatccg gacgcacctt
     8521 ccggaacatg ttcatctcgt accgcacctt cggcacgctc aatgccgaca agtccaacgc
     8581 gatcctctac cccacctcgt tctcggccca gcacaccgac gtcgagcaca tggtgcgcgt
     8641 ggacggcggg ctcgacccgg gcaagtattt catcatcatc gccaaccagt tcggcaacgg
     8701 cctgtcgagc tcgccctcga acacgccctg gcccgacatc ggcacccgct atcccgacgt
     8761 cacctactac gacgccgccc gcgtgcaaaa gcaaatgctc gaagaactgt ggggcatcga
     8821 gaaactggcc ctggtctacg gctggtccat gggcgccatg caggcctacc actgggccgc
     8881 gatctttccc gacgcggtcg agcgcatcgc cgtggtctgc ggctcggcgc gctgcgcgcc
     8941 gcacaaccag gtctttctcg agggcgtgcg ccacgccctg atcgccgacc cggcctaccg
     9001 cgacggcgtc ttcaccgaga agcccgtgcg cggcctgcgc gccatgggcc gcgtctacgc
     9061 cggctgggcc atgtcgcaga ccttctaccg ccaggaactg tggcgcacct tcggctgcag
     9121 ctcgctggaa gactacctgg tctcgcaatg ggaagtcacc tacgcccgcc gcgacccctg
     9181 cgacctgctc gcccatctct ggacctggca gcacggcgac ctcagcgcca acgagctcta
     9241 ccacggcgac ctgccgcgcg ccctggccgc catcaaggcc cgcgtgctgc tcatgcccgg
     9301 cgaccacgat ttgtacttcc aggtggccga caacgaactg gagctgccgc acctcaagca
     9361 cggccagctc aagcccatcc cctcgatatg gggccaccgg gccggcaacc ccatgaacca
     9421 gcccgaagac cgggccttca tcgacaacaa cgtgaaggcg ctgctggctt cctgacccgt
     9481 tcccccacat ccggcccgcg agaccgacat ggaaaaaaac gcaccctttc gcctcaaggc
     9541 ccaggatgtc atgcccctgt cggccaagtc ggacctaccc ggcctgcttc gagccagcgg
     9601 gcacctcggc gccatcgtgc tcgccgacat ggccctctgg catgcgctgg gcacctggtg
     9661 ggccgtgccg ctgaccttcc tgtccgggta tgtcgtcgcc ttcctgttct gcgtcgaaca
     9721 cgagaccgcc caccagacgg ctttccgcac gcgcgccctc aactacgtgt tcggccacct
     9781 ggcgagcctg gccatcctct acccctacga gtactaccgc gccttccact gggaccacca
     9841 ccgctacacc caggacccgg cccgcgaccc cgagctcgcc gtgccccaac ccgcctcgct
     9901 cgcgggcatc gcctgggcct gcgtgggcat ggccgccctg acgctgcgcg tgcgcaagct
     9961 gctggaacac gccgcgggcc gcgccaacga gccctgggtg cccgacaaca agcgcgcgct
    10021 cgtcgtgctc gaagcgcgct gctacctgct ggtttatgcc ctcgtcgccg tcgcatccat
    10081 cgccaccggc tcgctcgccg ccctctggtt gtggctgctg cccctgctgg ccggtcaatg
    10141 cctgatgcgg ccctacctca tgtccgagca cgtgggctgc ctccacgacc ccgacaccct
    10201 ggccaatacc cgcaccacct acaccaatgc cgtcgtgcgc ttcttcgcct ggaacatgcc
    10261 ctaccatgcc gagcatcaca cctacccgtc ggtgcctttt catgccctgc cgaagctgaa
    10321 cgtgttgctg gccgggcata ttgccaacac cgaacagggc tatatcgccg ccttctcggc
    10381 ggtgctgcgg caccttactc gccgcggcga agccgacgcc caggccaagg catgaggcgc
    10441 cgcttttaca gggtgggcca aaatacggta tagttgcggc cttgatttgg ggcggtagct
    10501 cagctgggag agcgtcgcgt tcgcaatgcg aaggtcggga gttcgatcct cctccgctcc
    10561 accagaaata cccagaaaaa tcaggccgtt agcgcaatgc tctcggcctg attttcattt
    10621 tcggcaatgg catttgccag aaatctgaca ttgccggcaa gggcgaaaag aagagagtct
    10681 ttgattcgat aagatcgata aacttgtcca gagacatata tcgccaaaga acatcactgt
    10741 ccttaaggct cttgcccacc ctaggcgttg ccaaaatcaa cctccttctt gtgtaaacat
    10801 gcaggaagca ccccataaga gtaccggacg cccacaccag gcgctgggtg cgttcctcac
    10861 aggctttgcg ctcttgctag gcactggcct cgatctaatg tttcaagaag gaggtacttc
    10921 ccctaatcgt gccgatcgcg cctgaaggcg tcaggcaggc cgggcctcgc ccagcatggc
    10981 cgccagcgcc gcagcgcgcg gcagtgcctc gatatccaat agttgctcgt gcaggcggtg
    11041 ggcggccgtc atgggcagca cgttgtccac gcaagcgaag aatttgcgcg acaggtcagc
    11101 ctcgccgagc atgcggggtg gatagccatg gggaatatcg acgctttccg agagtgtccg
    11161 gccatcgttc aggtatagcg tgacggtgga gctctcgcgt gaagcgctaa cgtcgccgga
    11221 gcctacttcc gtatcgacga tagtctcgaa gcgctcgatg agcgcgagca cgaaaggccg
    11281 tgtgatggcc tcggcagtga agctggccgg accaggcgcg ccgtcgacta gcgccgtgca
    11341 tacacaataa ggcaggctga aacgcgcctc ggaaacatcg gccggcacgc ggtatttcag
    11401 gttgcgcgaa gcgatgacgg acacgctggc ggtgatacgc acggcgcgtc ccatcacatc
    11461 gggataggca tgcgccagcg ccagcgccgc gtcgatggcg cggtgggcgc cagcacagca
    11521 cggatagggc ttggcccaaa taccgtattg cgtcatcgcc ggctcgccgt ccaggttggt
    11581 caacgcgccg gcaaagcccg gcgaaccggc tccgctagtc atttcccggt agccccaagt
    11641 gccctcgaag ggctcggcga tggcgctcac accggcctgc gccaggcgcg cggcgactag
    11701 gccgtttttg gccgccaggc ccgcatgcaa tggcttggcc atggtaccga actggcactt
    11761 ggagccgccg gccatgctgg tggccagcga aatcgccatg gcggtggcac gcgtatccag
    11821 tcgcagtagg cgggcgcacg ccgcggccac gccgggtgcg ccgagcgaca gcgtagtgtg
    11881 ccagccgcgg ccgtagtgat ccaggttcat ggcctcgccc agtcgcgtca tgacctcgaa
    11941 gccgaccaaa taggcttcca ggcaatcgtg accgctcgcg ccggtttcct cggccagcgc
    12001 gtacaacgcc ggcaccagcg cggcactcgg atgcgaacgg gccgggtcca acacgtcgtc
    12061 gtagtccagc gcatgcgcag cggtaccgtt gatgagcgcg gcccacggcg cagccagcat
    12121 agcggcttgt cccacgacat gcgctgcgcc gctgccccag gacaaggcgg ccgcggccgt
    12181 acggcgcact gccacttcac ccgagcctgc cagcatgacc gcggtggtgt ccaaaaaggc
    12241 gcgtcgcgcg ccgtccaggc aatcgcggtt ccagttgtga tcgggacggc tgcaccattg
    12301 cgccagacgg gctgtgggag aggcgtcaat atcagtcggc atggtgtcgt aatcctgggc
    12361 gcctcgcggc gcgttgaacg tgatgctccc tggcgagggt tcaggcagcc attagtgacg
    12421 acagcccgta cagtttgttg acgataaata gtgcaaccag ggagatgagc gtctgtacag
    12481 tggcgatagc ggcgatcgat gggtcgaact ggttctgcat atagctaagc atgaccactg
    12541 gcagcgtgtt ggtctcggcc gtgccgaaga agaacgagat cggcagattg tccagcgaga
    12601 tcagcagcgc gaacaccgag ccggcgaaga tacccgggag caccagcggt agcgtcacgc
    12661 gccgtaatgt ctcccacttg cccgcgccga ggatggcggc gctttcctcc aggtcgggtc
    12721 cgatgcctcg gtatacagac agaacggtgc gcgagatgta cgggatagca ataacggtgt
    12781 gggcgatcag tagcgacagg aatgatgtgc ccagcgacag cgccgacagg tagtacaaca
    12841 tggcgaaacc cagcaccacg gccgggattg agatgggcgc cagcagaacg actacgatgc
    12901 ccgaggtgat ccgcccgcgc atacggccca gtgccagcgc agcgggcacg gccagcaccg
    12961 cgccacaggc cgtcgaggcc agcgccagtt cgatgctcag cagcaacgaa tccaggaacg
    13021 gccggtagcg cacgatctgg gcataccagc gcagcgagaa cgactccacc ggcagcgaga
    13081 tgaacgacga tgacgtgaaa gatacgtaga cgacgacgag gatgggcgcg accagaaagg
    13141 ccagcagcac gatcatgtag gcggtcattg gccacatgcg tgcggaaacc ggcggtgcgc
    13201 cagcgggctt cgtgatcggg acagcactca tgcgtttacc cccttgcgcc gcagcaggcg
    13261 gatctggact atcaggcaga ccaacgcggc aaccaacagt acattgccca ttgcgctggc
    13321 aaatgcaatg tcatgcgtga gattgaactg ctggtagatc tgcagcggca gcgtgttgac
    13381 ctcgccgtcg cccaacaata ggttagccag gaaactgccg ttggtaacca cgaaaaccag
    13441 aacgcagccg ctggccacac cctccatgct caacgggaag gtaaccagga aaaaggcctg
    13501 ccaccagccc gcgccgagca cgcgcgcggc ctcctcgcag cgccgatcca cgcccttgag
    13561 cacggcggtg atcgacagga tcatgaaggg cagcataacg tgcaccagcg agagatagac
    13621 cgagaacggc atgcgcgcta gatcgaccgg gcgtgtgaac agcccgaacg ccaccagcag
    13681 tgcgctgacc ggcccatgtc gacccagcaa tatctgccaa ccaaatgtgc gcatgatgac
    13741 cgaagtcagg atcggcgcga tgagaaggaa gacgataacg ccgttccagc gtccggcatg
    13801 gcgcaccatg tagtacgagg ccggatagcc gaacaccagg cagcccagtg tgacaaccag
    13861 gctcagcttg agggtctcgg ccagagtacc cagataatag ggatcggtga acagtttatg
    13921 gtagtagaac caactgtcgt gccgggcgcc catggcggca ctgccattga tcaagacgtt
    13981 gtagagcagc gggccgatga agaagatcag caggaacgcc agcgccggca gcagccacgc
    14041 cgggcgcatg aggcgatagg aagcgcgggg cgccggagcg cctgccgcgc gccctgccct
    14101 ctcccgcgca ggccccacgg ccccgtccgc ggctggaccc tgcgcgcggc tcatcggggc
    14161 ggatcctcgt cgaccaggac gactgcgtcc tcggcgggcc aggtcagccc gacggactgc
    14221 ccaggggctg cagcgacacg gtcttggctg gtgatgaaga ccggcgctcc tcggcccggt
    14281 gcgcgcaatt cataggcgta gtggttgccg cgaaaaatca ctgcgctgat ctgcgcgccg
    14341 aaagccgccg cctcaccact ggcgaaagtg aagcgttccg gacgcaccgc cacgcgcacg
    14401 cgctggccga cgcgcgcctg cttcaagccg ccaacaggca gaacgcgctc tggcgcgatg
    14461 tccaagcgca cgcggccctg cccgttcgcg cctgcctcgt ccacggtggc gtcgaagata
    14521 ttggccgtac cgaggaattc cgccacaaac cgcgtgcgcg gccgggcgta aacttcagca
    14581 ggcgtgcccg cctgcacgat acggccttcg tgcatgacgg caatacggtc gctcatggtc
    14641 aacgcctctt cctgatcgtg cgtgaccatg acggaagtga tgcccaggcg ttgttgcagc
    14701 gagcgcaact cgaactgcat gcgctcgcgc aagtgcttgt ccagcgcgcc catcggttca
    14761 tcgagcaaca acaccgaagg ttgggtaacg atggcgcgcg ccagcgccac gcgttgctgc
    14821 tggcctcccg aaagctgatg gatatagcgg tcctcgaagc cctgcaggcg aacgagctgc
    14881 aaggcttcga tcaagcgctt ggtggcttcg cctgccggca ccttacgcat cctcaaccca
    14941 aaaccgatgt tctcggccac agtcatgtgc gggaaaagac tgtagctctg cgacatcatg
    15001 cctagcccac gctggttggg cggcaaggcg gtgatatctc gttcgccgat gcgcacgcta
    15061 ccggtgtcgg aggattcgaa accggccaca atgcgcagcg tggtcgtctt gccgcagccc
    15121 gaggggccga gcaaggcaag caattcgcct tcttccacct gcaggtccag aggatgcagc
    15181 gcctggttcg caccgaaacg cttactaatc gagcacagta ctaatgacga ggcgtcctgt
    15241 ttcatcggtg tgtcacgcat actgcgtcaa ttgccgacgg tcttgtgcca ctgctccacc
    15301 cacgcgccct ggtgcgcgtc gagctggtcg aacggaggcc agatgatctc cggcagcttg
    15361 gtaatgcgcg gggcgacctt gggcgagaac tgagtctggg tattggacat gccgtattgc
    15421 agcgcattgc cccagcaagc ttcaggttca ggcgagagca cgaagtcaat gaact
//
LOCUS       DASWPO010000191         9996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1740094, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000191 DASWPO010000000
VERSION     DASWPO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740094"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="VGC69_10075"
     CDS             <1..1990
                     /locus_tag="VGC69_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0295665.1"
                     /translation="TKDTMSTPFSTTNYTNELIQDKQAQSVADVLQNDPGVRLARGFG
                     NFQESYFIRGFILDSDSVSYNGLYGLLPRQYIASELFERVQVLRGASAFVNGMPPAST
                     ASGIGGSIDLLPKRAPIDPLTRVTTGVGSGGQASASADIARRFGPDDRLGIRLNAVAR
                     DGDTAVAGEHSRLGAGLLGLDWQGDRLRLSADLGWQDNRLRGTRPNVGVGALTSALPA
                     APSAGDNWAESWSYSNERDVFGTVRAEYDFNDNVTGWAAFGMRDGKEANSLSSLDLAN
                     AQGDGTTYRFDNTRTDFVHTGEIGLRAKARTGPIGHEFVLSASYFEHTEKDAWAMSGS
                     LATNLYNPVQYSLANLPTLYSAGNLADPSLGAHTWSSSYAFGDTLSFLDDSVLLTLGL
                     RHQQITQRGYDYTSGALTSSYEASHNSPAAGLVFKVTPQVSLYANYAEALVQGETAPF
                     TFNGQAVANAGASLSPYVSRQKEAGVKYDGGTLGGSLAFFTTDRPRAYVNDALVFSSG
                     GTDRHRGVELTAFGEITPRVRVLGGATWLDARQADTVTASLNGNRVIGIPHLQANLGV
                     EWDVPGLNGVTLDGRVVYTGSVYADDANTLRLPSWTRLDAGLRYKTKLSGHDLTLRAR
                     VENLANRRYWASAGGYPDQGYLVAGMPRTFWLSASMDF"
     gene            complement(2058..3071)
                     /locus_tag="VGC69_10080"
     CDS             complement(2058..3071)
                     /locus_tag="VGC69_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0295666.1"
                     /translation="MAGHDPHHDHDDHDHDPSGHDHAHAGHSHGHGHAGHDHLHGVTD
                     QRRIGWAFVIIAVFMVVEIVGGVLSGSLALLADAGHMVSDTAALGFSWMAIHYGRRPA
                     TSHLSYGYKRLEVLAAFVNGCALFAIAAWIVIEAVHRFAAPVPVIGKTMLIVAFAGLA
                     ANIAAFMVLHGGNRENLNMRGAWLHVLGDLLGSVAAVVAAGVILGTGWMPIDPILSIV
                     VAVIILKSAWGIVKSSAHILLEGTPAGVSLADIKADLESHVPEIRNAHHIHAWSITGE
                     QHMVTLHVLPVGGVPARDAVGAVQRRLAARFQITHATVQVEEDACLDAPAHRDAAACC
                     LEH"
     gene            3192..3521
                     /locus_tag="VGC69_10085"
     CDS             3192..3521
                     /locus_tag="VGC69_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0295667.1"
                     /translation="MTEDQAVASLAALAHAQRLRVFRALVVAGLGGLTPSVLADHLGV
                     ARNALSFHLKELAHAGLVTVEQQGRNLIYRADFSRMNGLLGYLTEHCCQGGICEANAT
                     PSACQTC"
     gene            3535..3981
                     /locus_tag="VGC69_10090"
     CDS             3535..3981
                     /locus_tag="VGC69_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY0295668.1"
                     /translation="MKRFHVHLHVDDLNRNIAFYSGLFAAQPARVEADYAKWMLDDPP
                     VNFAISARGRAPGLDHFGIQVEDDAELAVLKTQARAAAAPILDEGETTCCYARSDKHW
                     VTDPQGIAWEHFHTLENMPRFNEAAAPAAAAACCAPAANAARKSCC"
     gene            3993..5048
                     /gene="arsB"
                     /locus_tag="VGC69_10095"
     CDS             3993..5048
                     /gene="arsB"
                     /locus_tag="VGC69_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEY0295669.1"
                     /translation="MQTPASMSVFERYLTVWVFLCIVAGILLGQAAPAAFQAIGRLQI
                     AQVNLPVGLLIWLMVIPMLLKVDFGAMGRMRRHWRGIGITLLVNWAVKPFSMAFLGWL
                     FVRQAFAPWLPAGQIDSYIAGLILLAAAPCTAMVFVWSRLTNGDPLFTLSQVALNDAI
                     MVFAFAPIVGLLLGLSSITVPWHTLLTSVALYIVIPVILAQAWRRALLRRGQAVLDAA
                     LSRIGPWSIAALLLTLVLLFAFQGQAILRQPLVIAMLAVPILIQVLFNAGLAYWLNRQ
                     AGEPHSVACPSALIGASNFFELAVAAAISLFGFQSGAALATVVGVLIEVPVMLLAVRV
                     VNRTRGWYGRGAAAQAQ"
     gene            5045..5923
                     /gene="arsN2"
                     /locus_tag="VGC69_10100"
     CDS             5045..5923
                     /gene="arsN2"
                     /locus_tag="VGC69_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HEY0295670.1"
                     /translation="MIANRIEDTSTMSTARIYHNPDCGTSRNVLALLRAAGIGPAVIE
                     YLVTPPSRQTLRALIADAGLTVRGALRIKGTPYKDLGLDDAALSDDQLIDHMLRHPLL
                     INRPFVVTERGTRLCRPSDLALDLLPADAAAELEKKEVKKEEGTPFLRDEPIAGDAPD
                     FVAALLDAGLPIGDLAESNRTFYAYRTPAGRLAGYGGFERYADNALLRSITVPPMNRG
                     QGIGRNLLALLQRRAYGLGARQAWLLAGTAAPFFAQAGFKAVAREQAPQAILATRQAA
                     DLCPANAALMKRTIAF"
     gene            6022..7350
                     /locus_tag="VGC69_10105"
     CDS             6022..7350
                     /locus_tag="VGC69_10105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0295671.1"
                     /translation="MTTQRQLKLGAFMRPVSIHTGAWRYPGAYRDANFNFAHLKRFAQ
                     QLEAARFDAFFMADHLALLNMPMDALKRSHTVTSFEPFTLLSALSQATEHIGLVATAS
                     TTFDQPFHIARRFASLDHLSNGRAGWNVVTTSNPDAALNFGLEEHMDHDERYARAREF
                     YDVVTGLWDSWADDAFIRDAEQGLYFDPSRLHTLDHKGKYLSVRGPLHIARPVQGWPV
                     IVQAGASEPGKQLAAETAEVVFTAQNSLEGGRRFYADVKGRMDKLGRSRDYLKILPGA
                     LVVVGDTLEEARAIRARLDSLVYYDSAIASLSIALGCDASTFDPDGPLPEDIPESNAT
                     RSARERAIETARREGLTVRQLAQRLGGYGGLAFVGTAASIADEMQEWLETEGSDGFNI
                     MFPWLPGGLDAFVDKVVPELQRRGIFRREYEGATLRENLGLPRPGNRFFP"
     gene            7392..8588
                     /locus_tag="VGC69_10110"
     CDS             7392..8588
                     /locus_tag="VGC69_10110"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HEY0295672.1"
                     /translation="MSTVQPRVLLILSALMSLASISTDIYLPAMPALATGLHAEISQV
                     ALTLSSFLIGFSLGQLIWGPISDRYGRRVPIAIGMVLFMAGSVGCALSDSVSQMLGWR
                     VVQAAGACAGPVLARAIVRDLYAREHAARMMSTLMLVMGIAPLVGPLLGGQILVYSSW
                     RGIFWFLVVLGGLILFSLRGLPETLTEERRATTPLSSAFRDYYDLVRDPRLLGCALSG
                     GFFYAGVYAFLAGTPFAYIQYYHVRPQLYGFLFGLNIVAMMLANFTNSRLVMRVGPER
                     MLRLGGWVVALAGVVLAVNALFGWGGLAGLVAPIFFYVGMNGFIVANSVAAALSAFPN
                     RAGSASSLVGAIHFGSGILSSAMVGWFADGTPWTMGWIMGLMGLGCLAAAAGSFGIQR
                     RRANAA"
     gene            8714..9796
                     /locus_tag="VGC69_10115"
     CDS             8714..9796
                     /locus_tag="VGC69_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY0295673.1"
                     /translation="MRRLIRALFGDEQGELRQKIAVLYLLLIGFNVAAWAWAFMQFHR
                     YPLLMGTSLLAWTLGLRHAVDADHIAAIDNVTRKLMQEGKRPISVGLTFSLGHSTIVA
                     LASVGIAMTALAMQSRMAAFKEIGGLVGTLVSTFFLFTIALMNLWILRSVVRTFRRVR
                     RGEPYADEDLDLLLAGRGPLSRLFRPLFGLITRSWHMYFLGFLFGLGFDTATEVGLLG
                     ISAAGAAQGMPLWSILVFPALFTAGMTLVDTTDSILMLGAYGWAFVKPIRKLYYNITI
                     TAISVAVALIVGGIEGLGLLADKLQLNGAFWDAVGGLNDNFGLIGYIVIAVFVGSWGL
                     SAAIYRIKRFEDLDIPIQPGAPSGRT"
     gene            complement(9806..>9996)
                     /locus_tag="VGC69_10120"
     CDS             complement(9806..>9996)
                     /locus_tag="VGC69_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295674.1"
                     /translation="VRAVLRAGILPQRFFDLLGCHDDASRQQHAALRRVLAGQALDQA
                     PEALFDFTLLQDDGGRPG"
BASE COUNT         1535 a         3569 c         3207 g         1685 t
ORIGIN      
        1 cacgaaagac acgatgagca cgccgttcag caccaccaac tacaccaacg aactcatcca
       61 ggacaagcag gcccaaagcg tggccgacgt gctgcagaac gacccgggcg tgcgcctggc
      121 ccgcggtttc ggcaacttcc aggagtcgta tttcatccgc ggcttcatcc tcgactcgga
      181 cagcgtgtcg tacaacggcc tctacggcct gctgccgcgc cagtacatcg ccagcgagct
      241 gttcgaacgg gtccaggtgc tgcgcggggc gtcggccttc gtcaacggca tgccgccggc
      301 cagcaccgcc agcggcatcg gcggctccat cgacctgctg cccaagcgcg cgcccatcga
      361 tccgctcacg cgcgtcacca cgggcgtcgg cagcggcggc caggcctcgg cgtcggccga
      421 catcgcgcgc cgcttcggtc ccgacgaccg cctgggcatc cgcctcaacg ccgtcgcgcg
      481 cgacggcgac acggcggtgg ccggcgaaca ctcccgcctg ggcgccggcc tgctcggcct
      541 ggactggcag ggcgaccgcc tgcgcctgtc ggccgacctg ggctggcagg acaaccgctt
      601 gcgcggcacg cgccccaacg tgggcgtcgg cgcgctgacc tccgcgctgc ccgcggcgcc
      661 ctcggccggc gacaactggg ccgagtcgtg gtcgtactcc aatgagcgcg acgtattcgg
      721 cacggtgcgc gccgaatacg acttcaacga caacgtcacg ggctgggccg ccttcggcat
      781 gcgcgacggc aaggaagcca actcgctgtc cagcctcgac ctcgccaacg cccagggcga
      841 cggcaccacc taccgtttcg acaacacccg caccgacttc gtccacaccg gcgagatcgg
      901 cctgcgcgcc aaggcgcgca ccggcccgat cggccatgaa ttcgtgctgt cggcgtcgta
      961 tttcgagcac accgagaaag acgcctgggc gatgagcggt tcgctggcca ccaacctcta
     1021 caacccggtg cagtattccc tggcgaacct gcccacgctc tattcggccg gcaacctcgc
     1081 cgacccgagc ctgggggcgc atacctggag cagcagctac gccttcggcg acacgctgtc
     1141 cttcctggac gactcggtgc tgctcacgct gggcctgcgc caccagcaga tcacccagcg
     1201 cggctacgac tacaccagcg gcgccctcac atccagctac gaagccagcc acaactcccc
     1261 ggccgccggc ctggtgttca aggtcacgcc gcaggtttcg ctgtacgcca actacgccga
     1321 ggccctggtg cagggcgaga ccgcgccctt cactttcaac ggccaggccg tggccaacgc
     1381 cggcgcctcg ctgtcgccct atgtgtcgcg ccagaaggaa gccggcgtca agtacgacgg
     1441 cggcacgctg ggcggcagcc tggccttctt caccaccgac cggccgcgcg cctacgtcaa
     1501 cgatgcgctg gtcttctcct ccggcggcac cgaccgccac cgcggcgtgg agctcaccgc
     1561 cttcggcgag atcaccccgc gcgtgcgcgt gctgggcggc gcgacctggc tggatgcgcg
     1621 gcaggccgac accgtcacgg cgtccctcaa cggcaatcgc gtgatcggca tcccgcacct
     1681 gcaggccaac ctgggggtcg aatgggacgt cccggggctg aacggcgtca cgctcgatgg
     1741 acgggtggtc tataccggca gcgtctatgc cgacgacgcc aacacgctgc ggctgccgag
     1801 ctggacgcgc ctggacgctg gcctgcgcta caagaccaag ctgagcggcc atgacctcac
     1861 cctgcgcgcc cgggtggaga acctcgccaa ccgccgctac tgggcctcgg cgggcggcta
     1921 ccccgaccag ggctacctgg tggcgggcat gccgcgcacc ttctggctca gcgccagcat
     1981 ggacttctga cccgatcggg cgcaaacggg cagggcagcg cagggcagtg gccgccgccg
     2041 ctgcgctgcg ctttttttta gtgttccagg cagcaagccg ccgcgtcgcg gtgagctggc
     2101 gcgtcgaggc aggcgtcttc ctcgacctgc accgtggcgt gggtgatctg gaaccgggcg
     2161 gccaggcgcc gctgcacggc gcccacggca tcgcgggccg gcacgccgcc gacaggcagc
     2221 acgtgcaggg tcaccatatg ctgttcgccc gtgatggacc aggcatggat gtgatgggca
     2281 ttgcgaatct cgggcacatg cgactcgaga tcggccttga tgtcggccag gctcacgccc
     2341 gccggcgtgc cttcgagcag gatgtgggcc gatgacttca cgatgcccca ggcgctcttg
     2401 aggatgatca cggccaccac gatggacagg atagggtcga tcggcatcca gccggtgccc
     2461 aggatcacgc ccgcggccac cacggcggcc accgatccca gcagatcccc cagcacatgc
     2521 agccaggcgc cgcgcatatt gaggttctcg cggttgccgc cgtgcagcac catgaaggcc
     2581 gcgatattgg ccgccaggcc cgcgaaggcg acgatcagca tggtcttgcc gatgacgggc
     2641 accggcgcgg cgaaacggtg gacggcctcg atcacgatcc aggcggcaat ggcgaaaaga
     2701 gcgcagccat tgacgaaggc cgccagcact tccagccgct tgtagccgta cgacagatgc
     2761 gaggtggccg ggcggcggcc gtagtggatg gccatccagc tgaaacccag cgccgccgtg
     2821 tcgctcacca tgtggcccgc gtcggccagc agggcaagcg accccgagag cacgccgccg
     2881 acgatctcca ccaccatgaa cacggcgatg atgacgaagg cccagccgat gcggcgctgg
     2941 tccgtcaccc cgtgcaggtg atcgtggccg gcgtggccgt ggccatggct gtgcccggca
     3001 tgggcgtgat catgaccgct agggtcgtga tcatggtcgt catgatcgtg gtgagggtcg
     3061 tggccggcca tgggtgcgct cgctcagatg caggtgttcg tagtttacaa agcgcgaagc
     3121 gatcttcttg cgggaaaaga acaatatccc ctataattca attaacgttg aattattgaa
     3181 tcaatagcgc catgaccgaa gaccaagccg ttgcctccct tgccgcgctg gcccatgccc
     3241 agcgcctgcg cgtgttccgc gccctggtcg tcgccgggct gggagggctc acgcccagcg
     3301 tcctggccga ccatctcggc gtggcgcgca atgccttgtc ctttcacctg aaggaactcg
     3361 cccatgccgg gctggtgacg gtcgagcagc aaggccgcaa cctgatctac cgcgccgact
     3421 tttcccgcat gaacggcctg ctcggctacc tgaccgagca ctgctgccag ggcggcatct
     3481 gcgaggccaa cgccacgccg tccgcctgcc aaacctgctg accctaggta tcccatgaaa
     3541 cgcttccacg tccacctgca tgtcgacgac ctgaaccgca acatcgcctt ttactcgggc
     3601 ctcttcgccg cccagcccgc ccgggtcgag gcggactacg ccaagtggat gctggacgat
     3661 ccgccggtga atttcgcgat ttccgcgcgc ggccgcgcgc cgggcctgga ccacttcggc
     3721 atccaggtcg aggacgatgc cgagctggcc gtgctcaaga cgcaagcccg ggccgccgcg
     3781 gcgcccatcc ttgacgaagg ggagacgact tgctgctacg cgcgcagcga caagcattgg
     3841 gtcaccgacc cccagggcat cgcctgggag cacttccaca cgctggaaaa catgccgagg
     3901 ttcaacgagg ccgccgcccc ggcggccgcc gcggcctgct gcgcgccagc tgccaacgca
     3961 gcccgcaaat cctgctgttg agacccggct tcatgcaaac tcccgcatcc atgagcgtgt
     4021 tcgagcgcta cctcacggtc tgggtcttcc tgtgcatcgt cgccgggatc ctgctcggcc
     4081 aggccgcgcc ggccgcgttc caggccatcg gccgcctgca gatcgcgcag gtcaacctgc
     4141 cggtgggcct gctcatctgg ctcatggtca ttcccatgct gctgaaagtg gatttcggcg
     4201 ccatggggcg gatgcgccgg cattggcgcg gcatcggcat cacgctcctc gtcaattggg
     4261 ccgtcaagcc gttttccatg gccttcctgg gttggctctt cgtgcggcag gcattcgcgc
     4321 cgtggctgcc ggcgggccag atcgacagct acattgccgg actgatcctg ctggcggccg
     4381 cgccctgcac ggccatggtg ttcgtctgga gccggctgac caacggcgat ccgctcttca
     4441 cgctgtccca ggtggccttg aacgacgcca tcatggtgtt tgccttcgcc cctatcgtcg
     4501 gactgctgct ggggctgtcg tccatcacgg tgccgtggca tacgctgctg acttcggtgg
     4561 cgctctacat cgtcatcccg gtcatcctcg cccaggcctg gcgccgcgcg ctgctgcgcc
     4621 gcgggcaggc ggtcctcgac gccgcgctgt cgcgcatcgg cccctggtcg atcgccgcgc
     4681 tgctgctgac gctggtgctg ctcttcgcct tccagggcca ggccatcctg cggcagccgc
     4741 tggtcatcgc catgctggcc gtccccatcc tgatccaggt gctgttcaac gccgggctgg
     4801 cctattggct caaccgccag gcaggagaac cgcacagcgt ggcctgtccg tcggcgctga
     4861 tcggcgcatc gaatttcttc gagctggccg tggcggcggc catcagcctg ttcggtttcc
     4921 agtccggcgc cgcgctggcc accgtggtgg gcgtgctgat cgaagtgccc gtcatgctgc
     4981 tggcggtgcg ggtcgtcaac cgcacccgcg gctggtacgg gcggggcgcc gccgcgcagg
     5041 cccaatgatc gcaaacagga tcgaggacac ctccaccatg agcaccgcca ggatctacca
     5101 caacccggat tgcggcacgt cccgcaacgt gctggccttg ctgcgcgcgg ccggcatcgg
     5161 gcctgccgtc atcgaatacc tcgtcacgcc gcccagccgc cagacgctgc gggcgctgat
     5221 cgccgatgcc ggcttgaccg tgcgcggcgc cttgcgcatc aagggcacgc cgtacaagga
     5281 tctgggcctg gacgatgccg ccctgagcga cgaccagctc atcgaccaca tgctgcggca
     5341 ccccctcctg atcaaccggc ctttcgtcgt caccgagcgg ggcacgcggc tgtgccgtcc
     5401 gtcggacctg gcgctcgacc tgctgcccgc cgacgccgcg gccgagctcg agaaaaaaga
     5461 ggtcaagaaa gaggagggca cccctttcct gcgcgatgaa cccatcgccg gcgacgcgcc
     5521 ggatttcgtc gccgcgctgc tcgatgccgg attgcccatc ggcgacctgg ccgaatccaa
     5581 ccggaccttc tacgcctatc gcacacctgc cggccgcctg gccggctatg gcggattcga
     5641 gcgctacgcc gacaacgcgc tgctgcgctc catcaccgtg ccgccaatga accggggaca
     5701 gggaataggg cgcaacctgc tggcgctgct gcagcgccgc gcctacggcc tgggcgcccg
     5761 ccaggcctgg ctgctggccg gcacggcggc gccgttcttc gcgcaggcgg gtttcaaggc
     5821 cgttgccagg gagcaggcgc cgcaggccat cctggccacc cggcaagccg cggatctgtg
     5881 cccggccaat gccgcgctga tgaagcggac cattgcattc tgaggcaacg ccttgaagca
     5941 aacgccagcc cggcgcgccc ggttatatac ggcgcgcgat tttttcttaa acccgtcaag
     6001 acgccgcagt atgcttgcct catgactacc caacgacaac tcaagctggg cgctttcatg
     6061 cgcccggtca gcatccacac cggcgcctgg cgctatcccg gcgcgtatcg cgatgccaat
     6121 ttcaacttcg cccatctcaa gcgcttcgcg cagcagctgg aggccgcgcg cttcgacgcc
     6181 ttcttcatgg ccgaccacct ggcgctcttg aacatgccga tggacgcgct caagcgcagc
     6241 cacaccgtga cctcgttcga gcccttcacg ctgctctcgg cgctgagcca ggccaccgaa
     6301 cacatcggcc tggtggcgac cgcctcgacc accttcgacc agcctttcca catcgcccgc
     6361 cggttcgcct cgctcgacca cctgagcaac ggccgggcgg ggtggaacgt cgtcaccacc
     6421 tccaaccccg acgccgccct gaacttcggg ctggaagagc acatggacca cgacgagcgc
     6481 tacgcccggg cccgcgagtt ctacgacgtc gtgaccggcc tgtgggacag ctgggccgac
     6541 gacgccttca tccgcgatgc ggagcagggc ctgtacttcg acccgtcgcg cctgcatacg
     6601 ctggaccaca agggcaaata cctgtcggtg cgcgggccgc tgcacatcgc gcggccggtg
     6661 cagggctggc cggtcatcgt gcaggccggg gcctccgagc ccggcaaaca actggccgcc
     6721 gaaaccgccg aggtcgtctt caccgcgcag aattcgctgg aagggggccg ccgcttctac
     6781 gccgacgtga aagggcgcat ggacaagctc ggccgcagcc gcgactacct caagatcctg
     6841 ccgggcgcgc tggtcgtggt gggcgatacc ctcgaagagg cccgcgccat ccgcgccagg
     6901 ctcgacagcc tggtgtatta cgacagcgcc atcgcctcgc tgtccatcgc cctgggctgc
     6961 gacgcctcga cattcgaccc cgacggcccc ctgcccgagg acatccccga gagcaacgcc
     7021 acccgcagcg cccgcgaacg cgcgatcgaa acggcgcggc gcgaaggcct gaccgtgcgc
     7081 cagctggccc agcgcctggg cggctacggc gggctggcct tcgtgggcac ggcggcttcc
     7141 atcgccgacg agatgcagga atggctggag accgaagggt cggacggctt caacatcatg
     7201 ttcccgtggc tgcccggcgg cctggacgcc ttcgtcgaca aggtcgtgcc ggaactgcag
     7261 cgccgcggca tcttccggcg cgaatacgaa ggcgcgacgc tgcgcgagaa cctggggctg
     7321 cccaggccgg gaaaccggtt ttttccttaa ttaagtgttg cgtcattggc cgagtggttt
     7381 aatgacgggc tatgtccact gttcaaccac gcgtgctgct catcctcagc gcgctcatgt
     7441 cgctcgcctc gatctcgacc gacatttacc ttcccgcgat gcccgcgctg gccaccggcc
     7501 tgcatgctga aatcagccaa gtcgcgctca cgctttcatc ctttctgatc ggtttttccc
     7561 tggggcagct gatatggggc cccatcagcg accgctacgg ccggcgggtg ccgatcgcca
     7621 tcggtatggt cctgttcatg gcgggatcgg taggctgcgc cttgtccgat tcggtctccc
     7681 agatgctggg ctggcgcgtg gtgcaggccg ccggcgcctg cgcgggaccg gtgctggcgc
     7741 gcgcgatcgt gcgcgacctc tatgcgcggg aacatgcggc gcgcatgatg tccacgctca
     7801 tgctggtcat gggcatcgcg ccgctggtcg ggccgctcct gggcgggcag atcctggtct
     7861 attcgtcctg gcgcggcatc ttctggttcc tggtcgtcct gggcggcctg atcctgttct
     7921 ccctgcgcgg cctgcccgaa accctgacgg aggaacgccg cgccaccacg ccgctgagca
     7981 gcgcgttccg cgactattac gacctcgtcc gcgacccccg gctgcttggc tgcgccttgt
     8041 cgggcggttt cttctatgcc ggggtctatg ccttcctggc cggcacgccc tttgcctaca
     8101 tccagtacta ccacgtgcgg ccgcagctct acggctttct gttcgggctg aacatcgtcg
     8161 ccatgatgct cgccaatttc accaactccc ggctggtgat gcgggtcggc cccgaacgca
     8221 tgctgcgcct gggcggctgg gtcgtggcgc tggccggcgt ggtcctggcc gtcaatgccc
     8281 tgttcggctg gggcgggctg gccggcctgg tcgcgccgat cttcttttac gtgggcatga
     8341 acggcttcat cgtggccaac tcggtcgcgg ccgcgctgtc ggccttcccg aatcgcgccg
     8401 gctcggcctc gtcgctggtg ggcgccatcc atttcggctc cggcatcctc agttcggcga
     8461 tggtcggctg gtttgcggac ggcacgccct ggaccatggg ctggatcatg ggcctgatgg
     8521 gcctgggctg cctggccgcg gcggcggggt ctttcgggat acagcggcgc agggccaacg
     8581 ccgcgtgatc gccgggcagg ccatgggcgt cgtcaacccc atgctcaccc gcatatagga
     8641 tttttctcga aaaggtatta tctgctgcgg aaccgggcga gaccggacca ttcgaacaag
     8701 gcggaaatac ctcatgcgcc gactgatccg cgcgctgttc ggcgacgagc aaggcgagtt
     8761 gcgccaaaaa atcgctgtcc tctacctgct gctgatcggc ttcaacgtcg cggcatgggc
     8821 gtgggcgttc atgcaattcc atcgctaccc gctgttgatg ggcaccagcc tgctggcctg
     8881 gacgctcggg ctgcgccacg ccgtcgacgc cgaccacatc gccgccatcg acaacgtcac
     8941 gcgcaagctg atgcaggaag gcaagcgccc gatttcggtc ggcctgacgt tctcgctcgg
     9001 gcattccacc atcgtggcgc ttgcctcggt gggcatcgcc atgacggcgc tcgccatgca
     9061 aagccgcatg gcggccttca aggaaatcgg cgggctggtc ggcacgctgg tctccacctt
     9121 cttcctgttc acgatcgcgc tgatgaacct gtggatactc cgctcggtcg tgcgcacgtt
     9181 ccgccgcgtg cggcgcgggg agccctacgc cgacgaggat ctcgacctgc tgctggccgg
     9241 ccgcgggccg ctctcgcggc tgttcaggcc gctcttcggg ctcatcaccc gcagctggca
     9301 catgtatttc ctcggtttcc tgttcgggct gggcttcgac acggcgaccg aggtggggct
     9361 gctgggcatc tcggccgccg gcgcggcgca gggcatgccg ctctggtcca tcctggtgtt
     9421 tcccgcgctc ttcaccgccg gcatgacgct ggtcgacacg accgatagca tcctgatgct
     9481 gggcgcctac ggctgggcct tcgtcaagcc gatccgcaag ctctactaca acatcaccat
     9541 taccgcgatc tccgtcgcgg tggccttgat cgtcggcggc atcgaggggc tgggcctgct
     9601 cgccgacaag ctccagttga acggggcctt ctgggacgcg gtcggcggcc tgaacgacaa
     9661 cttcgggctc atcggctata tcgtgatcgc cgtgttcgtc ggcagttggg ggctgtccgc
     9721 ggcgatctac cggatcaagc gcttcgaaga cctcgacatc ccgatccagc ccggcgcgcc
     9781 atccggccgg acctagcgga acgcgtcagc ccggccggcc gccgtcgtcc tgcagcagcg
     9841 tgaagtcgaa gagcgcttcg ggcgcctggt ccagcgcctg gccggcgagc acgcggcgca
     9901 gcgccgcgtg ctgctgccgg gatgcatcgt catggcaccc gagcaagtcg aaaaagcgct
     9961 gcggcaggat gccggcgcgc agcactgccc gcaccc
//
LOCUS       DASWPO010000192         9400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2721851, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000192 DASWPO010000000
VERSION     DASWPO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721851"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1584
                     /locus_tag="VGC69_10125"
     CDS             76..1584
                     /locus_tag="VGC69_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0295675.1"
                     /translation="MNPTTSAAPPVPCRRPALLLLLTALALTGCTVGPDYVRPAVDAP
                     ASYKEQGPWKTAQPGNIDDRQDWWKSYGDSTLDQLMDQANAANLTVQVAMAQYRQARA
                     EDQAARSSFWPQLGLSAGPSRARTNTGGTDGGTKLANAYAIGLDASWEPDLWGEVRRS
                     VEASTATAQASAAQLAAARLSIQAELAQDYLQLRVTDQQKALYARTLAAYTRSLALTR
                     SQYEAGVTLRSDVALADAQLKTTQAQSIDLDAQRNQLEHAIAILIGKPPAEFSLPPAD
                     GWRASLPQTPAGVPSQLLERRPDIANAERLAAAANANIGVAQAAYYPSLTLSAGGGFS
                     APGGGLAQWFSVPNRVWALGASLAETLFDGGLRDAHTDAARAAYDAAVAQYKQTVLGG
                     FQEVEDNLSTLRVLGDENTAQDQAVDASRLAERLALSQYRAGTTTYLTVVTVQAQTLS
                     NERTSSELLGRQLVASVALVKAVGGGWKASQLDGEAAAAQTEQAAADGKRAD"
     gene            1611..2786
                     /locus_tag="VGC69_10130"
     CDS             1611..2786
                     /locus_tag="VGC69_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0295676.1"
                     /translation="MMAKPSFPRRAVLAAGAAALAAAGLWQAVRPVHAADTPAPKDPP
                     VLVRSATVQQQDVPIYLTGVGTVTANQSVTVKVRVDGQLDNVAFTEGQDVQAGQLLAQ
                     IDPRALQAQLQQAVATQAKDQAQLVNARRDMQRYAKLIKEDAATQQQIDTQTALVSQL
                     EAAVQMDAAQVSYAKVQLGYATITAPIGGRTGVRLIDQGNIVHATDNTGLVVINQIDP
                     IAVLFTLPEDAVTEINRILSTGGQALAVAALKRDTNQPLGQGKLMLVNNQIDTSSGTV
                     QLKALFPNPAHALWPGQYVNARLQLGLRKQALTIAAPAVQRGQDGTYVYTLDDNRVAR
                     LQPVHVADIQDGTAVIDQGVKAGDRVVIDGQYKLKPGLRTVEAKTDATDAGDTGAGK"
     gene            2783..6019
                     /locus_tag="VGC69_10135"
     CDS             2783..6019
                     /locus_tag="VGC69_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0295677.1"
                     /translation="MSISAAFIKRPIGTSLLAAALLLVGVAAWPLLPIAPLPQVDFPT
                     MQITVNLPGGSPETMASNVAQPLERQFALIAGLSQMTSVSAQNQSQITLQFDLDRNID
                     LAAVDVQAAINAASGQLPTNLPSSPSYRKVNPADAPVMLLSVQSKTLPITQVNDYADN
                     ILAQQISQIKGVGLVNIGGQQKPAVRIQVDPEKLRALGLSLEDIRGVIADTTVNQPKG
                     TLDGPAQNFTAYTNDQLLKAAPWNDMVLAYKNGAPIRVRDVGVAVDAAENNKLAGWAF
                     TGGSPVPGNTITNGRGVVLQITKQPGANVIETVDAINAAMPQLRAEIPPTVEVNTIID
                     RTQNIRASVKDVEFTLLLTILLVVMIIFVFLRDLAATLIPCITVPLALMGTAAFMYVL
                     GYSLDNLSLMALTIAVGFVVDDAIVMLENIYRYVEEGMNPLEAAYKGASEIGFTIMSI
                     SVSLIAVFIPLLLMGGIVGRLFREFAVTVTLTIVVSVAVSLTLTPMLCSRYLRNQHGR
                     PHSRLFDLFERGFDAMLNGYKRGLQFVLKHQFATLLVFLGTMAFTGLMFVTMPKGFFP
                     QQDTGFIYGFAQSAQDSSFSSMNERMLQLSDVLRKDKDITAFGMNGNQTQFNTGNFYI
                     NLRSKDKGRTATPDEIIRRLRPQLAKVPGVTLYMQAGQDINVGGRLSRTQYQYTLTDT
                     SLEELNQWAPKLLTRFSQLPQLTDVASDQQSNAPAANLTIDRARASSFGITPAMIDST
                     IYDAIGQRQVTQYFTQLNSYHVVLEVTPALQKDPSLFDKLYVTSPINNEQIPLSSFVH
                     LDTTKTAYLAINHQGQFPAVTLSFNLAPGASLGDAVNAINAAKAQMGVPDTLVGAFQG
                     TAKAFGDSLRSQPYLIAAALIAVYIVLGLLYESYIHPITILSTLPSAGLGALLILRAG
                     GFDLSVIALIGIILLIGIVKKNGIMMIDFALHAEREKGMSPREAIYQACLLRFRPIMM
                     TTMCALLSGLPLMIGNGQGAELRRPLGYAMVGGLVVSQMLTLFTTPVVYLYLDRAHFW
                     YMKRKEARAARKRGAGTGEHGGGHGAGSHGQHGEPPAAPSGAQTVVQK"
     gene            6050..6736
                     /locus_tag="VGC69_10140"
     CDS             6050..6736
                     /locus_tag="VGC69_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEY0295678.1"
                     /translation="MKILIIEDEVKTADYLRKGLTEQGCAVDVAHNGIDGRHQALEHD
                     YDVIVLDAMLPGMDGFEVLRSLRLVRQTPVIMLTARDSLEDRIKGLHDGADDYLVKPF
                     SFLELLARLQALTRRGRAQEPMQLRIGDLQIDLASRKAQRGGVRIDLTAKEFALLAVL
                     ARRKGDILSKTAIAELVWDMNFDSSVNVVEVAIKRLRAKIDGPFNTKLLHTIRGMGYV
                     LEPRDETGVA"
     gene            6746..8110
                     /locus_tag="VGC69_10145"
     CDS             6746..8110
                     /locus_tag="VGC69_10145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEY0295679.1"
                     /translation="MKPSITTRLAVIFASVALVTFTLIGVGLYAVLYSELLRQQTDVL
                     NTTANDIQYSLARIGTLDRWGRLKTRLDTLTTSDGSLRFWVISPDARFDYGSDPPSGD
                     SMAEGFGHLVRPDREYPMRTLTRMIPPIDDRPRVKLVIGIETAPYFHTLHTFLSALAV
                     LLMLAVLLILLLSQWVAKTALRPLRRLSNEAQQLSPRHLSQRLRTDRLPIELADLTAA
                     FNGALSRLEAAYKQLEAFNADVAHELRTPLTNLIGHTQVALSRRRSAKDLEEVLQTNL
                     EDLDQLRAIVNDMLFLARADQGEAASGLVRTVLAGEVGKTIEFFEFVLDDLGLTVDIE
                     GDVEAKAAIDTARLRRAITNLLQNAIQHTQGGTRIVVRIQRLDSMVRIAVANPGKTIE
                     PQHLPRLFDRFYRVDPARHDDGGTHGHGLGLAIVKAVASMHGGSVFATSDAGLTTIGF
                     SVRA"
     gene            8205..9309
                     /gene="prfB"
                     /locus_tag="VGC69_10150"
     CDS             join(8205..8285,8287..9309)
                     /gene="prfB"
                     /locus_tag="VGC69_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY0295680.1"
                     /translation="MEAERQNQIAARLADYAQRERALRGYLDYDARAERLQVVNAELE
                     DPAVWNDPKHAQDLGREKKSLDDVVTTLTELSTGIADARELFELAAADGDDDTMLAVD
                     EDANGFQVRLEGLEFRRMFSNPADPLNCFLDIQAGAGGTEAQDWASMLLRQYLKYAER
                     KGFAASVMEESEGEIAGIKSATIKIEGEYAFGMLRTETGVHRLVRKSPFDSSGGRHTS
                     FASVFVYPEIDDSFEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHLPTNIVVQC
                     QNDRSQHRNRAEAMQMLKSRLYELEMRNRMAEQQKLEDSKTDVGWGHQIRSYVLDQSR
                     IKDLRTNVEISNTQKVLDGDLDPFIQASLKQGV"
BASE COUNT         1696 a         3460 c         2942 g         1302 t
ORIGIN      
        1 cgactggcat ataactgacc gaaatgtcag ttgctcgaca gcgtgcggta ccgcagcgtt
       61 tcatacagtc gagccatgaa ccctacgaca tccgctgcgc cgcccgtccc gtgccggcgc
      121 ccagccctgc tcctgctgct gacagccctg gcgctgaccg gctgcacggt cggaccggac
      181 tatgtgcgcc ccgccgtcga cgcgcccgcc tcttacaagg agcaaggccc ctggaaaacg
      241 gcccagcccg gcaatatcga cgaccggcag gactggtgga aaagttacgg cgacagcacc
      301 ctggatcaat tgatggacca ggccaatgcc gcgaacctga ccgtgcaggt ggccatggcg
      361 caataccgcc aggcgcgcgc cgaggaccag gccgcgcgct cgtcgttctg gccgcagctg
      421 ggcctgagcg ccggcccgag ccgcgcccgc accaataccg gcggcaccga cggcggcacc
      481 aagctggcca acgcctatgc catcggcctg gacgccagtt gggagcccga cctgtggggc
      541 gaggtgcgcc gttcggtgga agccagcacc gccacggcgc aggcttcggc cgcgcaacta
      601 gccgccgccc ggctgagcat ccaggccgag ctggcccagg actacctgca gttgcgcgtc
      661 accgaccagc agaaagccct gtacgcgcgc accctggccg cctacacgcg ttcgctggcg
      721 ctcacccgca gccagtacga ggccggcgtc acgctgcgct cggacgtggc cctggccgac
      781 gcccagctga agacgacgca ggcccagtcc atcgacctgg atgcccagcg caaccagctc
      841 gaacacgcca tcgccatcct gatcggcaag ccgccggcgg aattctcgct gcccccggcg
      901 gatggctggc gggccagcct gccgcagacg cccgccggcg tgccctcgca actgctggag
      961 cgccggcccg acatcgccaa cgccgaacgc ctggccgcgg cggccaacgc caatatcggc
     1021 gtggcgcagg cggcctacta ccccagcctg acgctgagcg ccggcggcgg cttcagcgcc
     1081 ccgggcggcg gcctggcgca atggttcagc gtgcccaacc gcgtctgggc gctgggcgcc
     1141 tcgctggccg agacgctgtt cgacggcggc ctgcgcgatg cgcacaccga cgccgcccgc
     1201 gccgcctacg acgccgcggt ggcgcagtac aaacagaccg tgctgggcgg cttccaggaa
     1261 gtcgaggaca acctctccac cctgcgggtg ctgggcgacg agaacaccgc ccaggaccag
     1321 gccgtggacg cctcgcgcct ggccgagcgg ctggccctga gccaataccg ggccggcacc
     1381 acgacctatc tcaccgtcgt cacggtgcag gcgcagaccc tatccaatga acggacctcg
     1441 tccgagctgc tgggccgcca gttggtggcc agcgtggcgc tggtcaaggc ggtcggcggc
     1501 ggctggaagg ccagccagct cgacggcgag gccgccgcgg cgcagacgga acaggcggcc
     1561 gcggacggca agcgcgccga ttgaacgcac atcagactcg agagcagaaa atcatggcta
     1621 aaccatcgtt tccacgccgc gccgtcctgg ccgcaggcgc cgcggcgctc gccgccgccg
     1681 gcctctggca ggcggtccga ccggtccacg cggccgacac ccccgcgccc aaggacccgc
     1741 cggtgctggt ccgttcggcc acggtacagc agcaggacgt acccatctac ctcaccggcg
     1801 tgggcacggt gacggccaac cagtcggtca cggtcaaggt gcgcgtcgac ggccagctcg
     1861 acaacgtggc cttcaccgag ggccaggacg tgcaggcggg ccagttgctg gcgcagatcg
     1921 acccgcgcgc cctgcaggcc cagctgcagc aggccgtggc cacccaggcc aaggaccagg
     1981 cgcagctcgt caacgccagg cgggacatgc agcgctacgc caagctgatc aaggaagacg
     2041 ccgccaccca gcagcagatc gacacgcaga ccgcgctggt cagccaactg gaagcggcgg
     2101 tgcagatgga cgcggcccag gtcagctacg ccaaggtgca gctcggctac gcgaccatca
     2161 ccgcccccat cggcgggcgc accggcgtgc gcctgatcga ccagggcaat atcgtgcacg
     2221 ccaccgacaa caccggcctg gtggtgatca accagatcga ccccatcgcg gtgctgttca
     2281 ccctgcccga ggacgccgtg accgagatca accgcatctt gtcgacgggc ggccaggcgc
     2341 tcgcggtggc ggcgctcaag cgcgacacca accagccgct gggccagggc aagctgatgc
     2401 tggtcaacaa ccagatcgac accagcagcg gcacggtcca gctcaaggcg ctgttcccca
     2461 acccggcgca cgccctgtgg cccggccagt acgtcaacgc gcggctgcaa ctgggcctgc
     2521 gcaagcaggc gctgacgatc gccgccccgg ccgtgcagcg cggccaggac ggcacctacg
     2581 tctacaccct cgacgacaac cgcgtggcca ggctgcagcc ggtgcacgtc gcggacatcc
     2641 aggacggcac cgccgtcatc gaccagggcg tcaaggccgg cgaccgcgtg gtgatcgacg
     2701 ggcagtacaa gctcaagccc ggcctgcgca ccgtcgaagc caagaccgac gcgaccgacg
     2761 ccggtgacac cggggcaggc aaatgagcat ttccgccgct ttcatcaaac gtcccatcgg
     2821 caccagcctg ctggcggcgg ccctgctgct ggtgggcgtg gccgcctggc cgctgctgcc
     2881 gatagcccca ctgccgcagg tcgacttccc gaccatgcag atcaccgtca acctgccggg
     2941 cggcagtccg gagaccatgg cgtcgaacgt ggcccagccg ctggagcggc agttcgccct
     3001 gatcgccggc ctgtcgcaga tgacctcggt gagcgcgcag aaccagtcgc agatcaccct
     3061 gcagttcgac ctggaccgca acatcgacct ggcggccgtc gacgtccagg cggccatcaa
     3121 cgcggcctcg ggccagttgc ccaccaacct gcccagctcg ccgtcctacc gcaaggtcaa
     3181 tccggccgac gcgcccgtca tgctgctgag cgtgcagtcc aagaccctgc ccatcaccca
     3241 ggtcaacgac tacgccgaca acatcctggc gcagcagatc tcgcagatca agggcgtggg
     3301 cctggtcaac atcggcggcc agcagaagcc cgcggtgcgc atccaggtcg accctgaaaa
     3361 gctccgcgcc ctgggcttgt cgctggaaga catccgcggc gtcatcgccg acaccaccgt
     3421 caaccagccc aagggcaccc tggacggccc ggcgcagaac ttcaccgcct acaccaacga
     3481 ccagctgctc aaggccgcgc cgtggaacga catggtgctg gcctacaaga acggcgcgcc
     3541 catccgcgtg cgcgacgtgg gcgtggcggt ggacgccgcc gagaacaaca agctggccgg
     3601 ctgggccttc accggcggct cgccggtgcc gggcaacacc atcaccaacg gccgcggcgt
     3661 cgtgctgcag atcaccaagc agcccggcgc caacgtgatc gaaacggtgg acgcgatcaa
     3721 cgcggccatg ccgcaattgc gcgcggagat cccgcccacg gtggaggtca acaccatcat
     3781 cgaccgcacg cagaacatcc gcgcctcggt caaggacgtg gagttcaccc tgctgctgac
     3841 catcctcctg gtggtgatga tcatcttcgt gttcctgcgc gacctggcgg cgaccctcat
     3901 tccctgcatt accgtgccgc tggcgctgat gggcaccgcc gctttcatgt acgtgctggg
     3961 atacagcctg gacaacctgt cgctgatggc cctgaccatc gcggtgggct tcgtggtcga
     4021 cgacgccatc gtgatgctgg agaacatcta ccggtacgtc gaagagggca tgaatccgct
     4081 ggaggccgcc tacaagggcg ccagcgagat cggcttcacc atcatgtcga tctcggtgtc
     4141 gctgatcgcg gtcttcatcc cgctgctgct gatgggcggc atcgtcggcc ggctgttccg
     4201 cgaattcgcg gtgaccgtca ccctgaccat cgtggtgtcg gtggcggtgt cgctcacgct
     4261 cacgcccatg ctgtgctcgc gctacctgcg caaccagcac ggcaggccgc acagccgcct
     4321 gttcgacctg ttcgaacgcg gcttcgacgc catgctcaac ggctacaagc gcggcctgca
     4381 gttcgtgctc aagcaccagt tcgccaccct gctggtcttc ctgggcacga tggcgttcac
     4441 cggcctgatg ttcgtgacca tgcccaaggg cttcttcccc cagcaggaca cgggattcat
     4501 ctacggcttc gcccagtcgg cccaggactc ctcgttcagc tccatgaacg agcgcatgct
     4561 gcaactgtcc gacgtgctgc gcaaggacaa ggacatcacc gccttcggca tgaacggcaa
     4621 ccagacgcag ttcaacaccg gcaacttcta catcaacctg cgctccaagg acaaaggccg
     4681 caccgccacg cccgacgaga tcatccgccg gctgcgcccg cagctcgcca aggtgccggg
     4741 cgtcacgctc tacatgcagg ccggccagga catcaacgtg ggcggccgcc tgtcgcgcac
     4801 gcaataccag tacacgctca ccgacaccag cctggaggag ctcaaccaat gggcgcccaa
     4861 gctgctgacg cgcttctcgc agttgccgca actgaccgac gtggcgtccg accagcagag
     4921 caacgcgccc gcggccaacc tgaccatcga ccgcgcccgc gcctccagct tcggcatcac
     4981 gccggccatg atcgacagca ccatctacga cgcgatcggc cagcggcagg tgacgcagta
     5041 cttcacccag ctcaacagct accacgtcgt gctggaagtc acgccggcgc tgcagaagga
     5101 cccgagcctg ttcgacaagc tgtacgtgac ctcgccgatc aacaacgagc agatcccctt
     5161 gtcgtccttc gtgcacctgg acaccaccaa gaccgcctac ctggcgatca accaccaggg
     5221 ccagttcccg gcggtgacgc tgtcgttcaa cctggcgccg ggcgcctcgc tgggcgacgc
     5281 ggtcaatgcg atcaacgccg ccaaggccca gatgggcgtg cccgacacgc tggtcggcgc
     5341 cttccagggc acggccaagg ccttcggcga ctcgctgcgc tcgcagccct acctgatcgc
     5401 cgccgcgctg atcgcggtgt acattgtgct gggcctgctg tatgagagct acatccaccc
     5461 gatcaccata ctctcgaccc tgccctcggc cggcctgggc gcgctgctga tcctgcgcgc
     5521 gggcggcttc gacctgagcg tgatcgcgct gatcggcatc atcctgctga tcggcatcgt
     5581 caagaagaac ggcatcatga tgatcgactt cgccctgcac gccgagcgcg agaagggcat
     5641 gtcgccgcgc gaggccatct accaggcctg cctgctgcgc tttcgcccca tcatgatgac
     5701 caccatgtgc gcgctgctca gcggcctgcc gctgatgata ggcaacggcc agggcgccga
     5761 gctgcgccgc cccctgggct acgccatggt gggcggcctg gtggtgtcgc agatgctgac
     5821 gctcttcacg accccggtgg tctacctcta cctggaccgc gcccacttct ggtacatgaa
     5881 acgcaaagaa gcccgcgcgg cgcgcaagcg cggcgccggc acgggtgaac acggcggcgg
     5941 gcacggcgcg ggatcgcacg ggcagcacgg cgaacccccg gccgccccat cgggggcgca
     6001 gacggtcgtg caaaaatagg aacatgcatc ctagctcccg gcccttgcca tgaagatact
     6061 gatcatcgaa gacgaggtca aaaccgccga ctacctgcgc aaggggttga cggagcaagg
     6121 ctgcgccgtc gacgtggcgc acaacggcat cgacgggagg caccaggcgc tggagcacga
     6181 ctacgacgtg atcgtgctcg acgccatgct gcccggcatg gacggcttcg aggtgctgcg
     6241 ttcgctgcgc ctggtgcgcc agacgccggt catcatgctg accgcccgcg acagcctgga
     6301 agaccgcatc aagggcctgc acgacggcgc cgacgactac ctggtcaagc ccttctcctt
     6361 cctggagctg ctggcgcgcc tgcaggcgct gacgcggcgc ggccgggcgc aggagcccat
     6421 gcagctgcgc atcggcgacc tgcagatcga cctggccagc cgcaaggcgc agcgcggcgg
     6481 cgtgcgcatc gacctgaccg ccaaggaatt cgccctgctg gcggtgctgg cgcggcgcaa
     6541 gggcgacatc ctctccaaga cggcgatcgc cgagctggtc tgggacatga acttcgacag
     6601 cagcgtcaac gtggtcgagg tcgccatcaa gcggctgcgc gccaagatcg acggtccgtt
     6661 caacaccaag ctgctgcaca cgatacgcgg catgggctac gtgctggagc cgcgcgacga
     6721 aaccggcgtc gcctgaggcc aggccatgaa gccgtccatc acgacccggc tggccgtcat
     6781 cttcgcctcg gtggcgctgg tgacgttcac gctgatcggc gtggggctgt acgcggtgct
     6841 ctacagcgaa ctgctgcgcc agcagaccga cgtgctcaac accaccgcca acgacatcca
     6901 gtattcgctg gcgcgcatcg gcacgctgga ccgctggggc cgcctgaaga cccgcctgga
     6961 cacgctgacc acctccgacg gcagcctgcg cttctgggtc atcagccccg atgcgcgctt
     7021 cgactacggc agcgatccgc cgtccggcga cagcatggcc gagggcttcg gccacctggt
     7081 ccggccggac cgggaatacc ccatgcgcac cctcacccgc atgatcccgc ccatcgacga
     7141 ccggccccgg gtcaagctcg tcatcggcat cgagaccgcg ccctattttc acaccctgca
     7201 caccttcctc agcgccctgg cggtcttgct gatgttggcg gtgctgctga tcctgctgct
     7261 cagccagtgg gtggccaaga ccgccctgcg ccccctgcgg cgcctgtcga acgaagccca
     7321 gcagctcagc ccccggcacc tgtcgcagcg cctgcgcacc gaccggctgc ccatcgagct
     7381 ggccgacctg acggcggcct tcaacggcgc gctcagccgg ctcgaggccg cctacaagca
     7441 gctggaggcc ttcaacgccg acgtggccca cgaactgcgc accccgctca ccaacctgat
     7501 cgggcacacc caggtggcgc tgtcgcggcg gcgcagcgcc aaggacctgg aagaagtgct
     7561 gcagaccaac ctggaagacc tcgaccagtt gcgcgccatc gtcaacgaca tgctgttcct
     7621 ggcgcgcgcc gaccagggcg aagcggccag cgggctggtg cgcaccgtgc tggccggcga
     7681 ggtcggcaag accatcgagt tcttcgagtt cgtcctcgac gacctggggc tcacggtgga
     7741 catcgagggc gacgtcgagg ccaaggcggc catcgacacg gcgcgcctgc gccgcgccat
     7801 caccaacctg ctgcagaacg ccatccagca tacgcagggc ggcacgcgca tcgtggtgcg
     7861 catccagcgc ctggacagca tggtgcgcat cgccgtggcc aacccgggca agaccatcga
     7921 gccgcagcac ctgccgcggc tcttcgaccg cttctaccgg gtcgacccgg cccgccacga
     7981 cgacggcggg acccacgggc acggcctggg tctggccatc gtcaaggcgg tcgccagcat
     8041 gcacggcggc agcgtattcg ccaccagcga cgccggcctg accacgatag gcttcagcgt
     8101 ccgggcctag gagcccgccg gaggcgcccg cgcccgcccg gccggccggc gcgctaaaat
     8161 agtctgtttc gcaccgcaaa aatccaagaa tcaggaaccg ctccatggaa gccgaacgcc
     8221 agaaccagat cgccgcccgc cttgccgact acgcgcagcg cgagcgggcg ctacgggggt
     8281 atctttgact acgacgcccg cgccgagcgt ctgcaagtcg tcaatgccga gctggaagac
     8341 ccagccgtct ggaacgaccc caagcacgcc caggacctgg gccgtgaaaa aaaatccctc
     8401 gatgacgtcg tcacgacgct gaccgaactc agcaccggca ttgccgacgc gcgcgagctt
     8461 ttcgagctgg ccgccgccga cggcgacgac gacacgatgc tggccgtcga cgaggacgcc
     8521 aacggcttcc aggtccgcct cgaaggcctg gaattccgcc gcatgttctc caacccggcc
     8581 gacccgctga actgcttcct ggacatccag gccggcgccg gcggcaccga ggcgcaggac
     8641 tgggcctcca tgctgctgcg ccagtacctc aagtacgccg agcgcaaggg cttcgcggcc
     8701 tccgtcatgg aagaatccga gggtgaaatc gccggcatca agtcggccac catcaagatc
     8761 gagggcgagt acgccttcgg catgctgcgc accgagaccg gcgtgcaccg cctggtgcgc
     8821 aagagcccct tcgactcctc gggcggccgc cacacctcgt ttgccagcgt gttcgtctac
     8881 cccgagatcg acgactcctt cgagatcgag atcaacccgg ccgacctgcg catcgacacc
     8941 taccgcgcca gcggcgcggg cggccagcac atcaacaaga ccgactccgc cgtgcgcatc
     9001 acccacctgc ccaccaatat cgtggtgcag tgccagaacg accgctcgca gcaccgcaac
     9061 cgcgccgagg ccatgcagat gctgaagtcg cgcctgtacg agctggaaat gcgcaaccgc
     9121 atggccgagc agcaaaagct cgaagactcc aagaccgacg tgggctgggg ccaccagatc
     9181 cgctcgtatg tgctggacca gagccgcatc aaggacttgc gcaccaacgt cgaaatttcc
     9241 aacacgcaga aggtgctgga cggcgacctg gaccccttca tccaggccag cctgaagcaa
     9301 ggcgtctagg cccctgcccc tccgaggaca cccgcatgac cgacaccgtt gcaatcctca
     9361 ccggcgcctc gcgcggcata ggcctcgcgc tggcgcgcgg
//
LOCUS       DASWPO010000193        11478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2007075, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000193 DASWPO010000000
VERSION     DASWPO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007075"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="tilS"
                     /locus_tag="VGC69_10155"
     CDS             complement(<1..813)
                     /gene="tilS"
                     /locus_tag="VGC69_10155"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY0295681.1"
                     /translation="MTDAERRLPDCPADLIACVRAALRGLPKDTAHIAAAVSGGADSA
                     MLALAAAHAARGLGIDLTLFHIHHGLLAQADDWAAHVQTLGALLGAPVLQARVRVPDD
                     TGKGIEAAARQARYEAFAGLARHAGVNHILLAHHQDDQAETVLLRLLRGAGLQGMAAM
                     AAQTERDGIRYLRPWLAVPRAALRDAAADFARAQGWQPVEDPTNTDARYTRAAVRTQL
                     KPALDARWPGWQAIVARHARHMAEAAEVLAEVAQADLAALDFSPADASFALAA"
     gene            complement(850..1815)
                     /locus_tag="VGC69_10160"
     CDS             complement(850..1815)
                     /locus_tag="VGC69_10160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY0295682.1"
                     /translation="MRNTFLEFEQPLAELENKIEQLRYVQADSAVDISDEIGRLQQKS
                     QTLAKEIYGKLTPWQTALVARHPQRPYTLDYVREIFTDFHELHGDRMYTDDQSIVGGL
                     ARFNGAACMVIGHQKGRDTKERAARNFGMPRPEGYRKALRLMRLAEKFNLPVFTFVDT
                     PGAYPGIGAEERGQSEAIGHNLYAMAELKVPVICTIIGEGGSGGALAIAVGNAVLMLQ
                     YATYSVISPEGCASILWRSAEKAPDAAEALAITAPRLKDLGLVDRVVNEPVGGAHRDP
                     RVMARLLRRALGDTLRQLQGMNPEELVEQRVQRLLSYGRFQEVRA"
     gene            complement(1861..2505)
                     /locus_tag="VGC69_10165"
     CDS             complement(1861..2505)
                     /locus_tag="VGC69_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY0295683.1"
                     /translation="MSSADIAIEKPDYWEDAVAHLVKRDRILKKIIPQHPQVWLTSRG
                     TPFITLARAIVGQQISVRAADAVWGKFIDTVGKRPAPAVVLKTGMEGLRAAGLSQRKA
                     EYLQDLAAHFSERRVRPEKWAAMQDEDVIDELVAIRGIGRWTAEMFLIFNLQRPDILP
                     LDDLGLLKAISLHYFSGEPVSRFEAREVSLAWQPWRTVATWYLWRSLDPTPVQY"
     gene            complement(2522..3970)
                     /gene="cysS"
                     /locus_tag="VGC69_10170"
     CDS             complement(2522..3970)
                     /gene="cysS"
                     /locus_tag="VGC69_10170"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY0295684.1"
                     /translation="MLQIYNSLSRAKTTFVPVETGKVRMYVCGMTVYDYCHLGHARML
                     VSFDVVQRWLRASGYAVEYVRNITDIDDKIIRRAVETNRRIGEVTSYYIDAMHADELA
                     LGVQSPDHEPRATQYVGEMLGIIAKLRENGLAYQADDGDVNYAVRGFAGYGKLSGKTL
                     DDLRAGERVAVGSAKRDPLDFVLWKSAKPEEPADTKWDSPYGPGRPGWHIECSAMSHA
                     LLGQPLDIHGGGPDLKFPHHENEIAQTEGAFGGTMANTWMHCGPLMVDADKMSKSLGN
                     FRTIRQTVADEAGLSDSQADYAVRAREAEMLRFFIVRNHYRSPQNFTPDNLLDAQSAL
                     DSLYQALRNVAPDWLNIDWTEPQAQAFKAAMDDDFNTSGAVAALFELASEANRTKSAR
                     AAGQLKQLGGLLGLLQQDPETYFQSVTRYRAGSAASAELDSAAIDALVAARAAAKQAR
                     NFTEADRIRAELHAAGIELDDKPGGITQWRRA"
     gene            4108..4755
                     /locus_tag="VGC69_10175"
     CDS             4108..4755
                     /locus_tag="VGC69_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0295685.1"
                     /translation="MPMSVTSMLRFRRAVLAAASLAVLAAAPPAAQAQSMGGGGALTS
                     NANLTTLDPYPPEKGWQGLSHALDHLRPGLDTRLQPSPSQITDHIEARISNGDYAGAL
                     SQIEQREAQLKGKPGTDVQLLFQHARVLAALGRTDEAIAIYTDMTTRFPELPEPWNNL
                     AALHAKQGNLDQARAALRMALRADPDYADAQANLKLLASRDPSADQPRTKEPPSQ"
     gene            4887..5396
                     /locus_tag="VGC69_10180"
     CDS             4887..5396
                     /locus_tag="VGC69_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0295686.1"
                     /translation="MSTQPRVKMETSLGDFVLTLDAEKAPKTTENFLTYVKEGFYDGT
                     IFHRVIDGFMIQGGGFEPGLKQKPTHSPIENEANNGLKNDKYTIAMARTSDPHSATAQ
                     FFINAANNDFLNFTAPTPNGWGYTVFGKVTEGTETIDKIKGVKTGNSGFHQNVPTEDV
                     LIKKAEIVE"
     gene            5389..6162
                     /locus_tag="VGC69_10185"
     CDS             5389..6162
                     /locus_tag="VGC69_10185"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY0295687.1"
                     /translation="MNRLVLPEGPVWLASDLHLSESTPATQEAFLGFLQAAAGEAAAL
                     LLPGDIFNTWIGDDVITDPEPWLAQALDGLKTAAARLPVYLGRGNRDFLMGRRLARAL
                     GAHLLPDSVLAKTAYGDVLLSHGDEYCTDDEAYQRWRRKSRNPIVQRIFLLRSLASRQ
                     DFARRAREKSMAAKRDTPPAIMDVNPQAVEKAFRESGARLMVHGHTHRPARHVLTVDG
                     RQCERWVLPDWDLDHAEPPRGGWLVIDADGPQFYDLESA"
     gene            6174..6821
                     /locus_tag="VGC69_10190"
     CDS             6174..6821
                     /locus_tag="VGC69_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY0295688.1"
                     /translation="MYTPAHFDESRPEALHDLITRHPLGMLVTHGKSGLDANHIPFEL
                     VPGEGPRGILRAHVSRNNPVWQDVANGDDVLVVFRAADAYISPTWYPSKHEAHKQVPS
                     WNYMVVHVHGRIAIRDDERYVRGVVARLTRAHEAGQPVPWKMTDSPAQFIDAMLRAIV
                     GIEIGITRFEGKFKLSQNKEARDIRGAGQALTQGGPDAVGEAMLACAAAKAAAEK"
     gene            complement(6828..8420)
                     /locus_tag="VGC69_10195"
     CDS             complement(6828..8420)
                     /locus_tag="VGC69_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0295689.1"
                     /translation="MAWLLDPTAWVGLLTLVVLEIVLGIDNLIFIAILADKLPPKQRD
                     RARIMGLGLALVTRLVLLTVMSWLVTLTRPLFSLGPLSFSGRDLILLVGGLFLLFKGT
                     VELHERLEGSIHADGLGKRIYASFWVIVTQIVVLDAIFSIDSVITAVGMVDHLAIMMT
                     AVVIAVGIMLVASKPLTRFVNAHPTVVVLCLGFLLMIGFSLLAESLGFKVPKGYLYAA
                     IAFSAAIESLNQVARRNLLRLDGVRPMRERTAETVMRMLGQRPLDVEPDVDVPPSAQA
                     AQKAFGVEERNMVSGVLTLAERSIHSIMTPRTDVSWINIEDDAAAIREQISAAPHSFF
                     PVCRGALDEIIGIGRAKDLVADLITEGHIDHSHLRDPIIVHEAIGILRLMDTLKRSRG
                     QLVLIADEFGAIVGLVTPIDVFEAIAGEFPDEDEMPDILVERANVWRIDGAADLHHVE
                     QVLETDGLVDDSQDYTTLAGYLLARFGHLPKPGDACEYEAHYMHFRFEVLQIEGRRIA
                     SVRVEKSHIEPEEPGDPHENLV"
     gene            complement(8462..9247)
                     /locus_tag="VGC69_10200"
     CDS             complement(8462..9247)
                     /locus_tag="VGC69_10200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0295690.1"
                     /translation="MHPMLNTAIKAARRAGTIINRASLDLDRLSVARKGPRDYVTEVD
                     RASEEAIVEALLAAYPDHAVLGEEYGRQGSEHSEFLWIIDPLDGTTNFIHGLPTYAVS
                     IALQHRGQITQAVVYDPSRNELFTASRGGGTFLNDRRVRVSGRIRFAEALLGAHWPGS
                     TNPGLGAPRFAGMAEGCSSVRRMGATVLDLAYVAAGRLDGFCGVGLKPWDLAAGSLLV
                     LEAGGLVSDFDGEQSWLDSGDVLAGTPKIFAQMVAGLQNKPAT"
     gene            9334..10119
                     /locus_tag="VGC69_10205"
     CDS             9334..10119
                     /locus_tag="VGC69_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0295691.1"
                     /translation="MTQAFSRVRFIMTQPSHPGNVGSAARAIKTMGFSELVLVEPKLP
                     DMAAQPEAVALASGAVDVLERSQVFDTLEEALAPATLAFALTARVRDLGPPPCDIRQT
                     AELARAHLAGTGQGCVAIVLGTERAGLTNAQIALCHRICHIPANPEYSSLNVAQALQL
                     AAWEMRYALLQETGAPLLPASTAQQPDPGAAPADGAAVQALLAHWEEALVAVQFLDPA
                     HPKKLMPRMRHLFSRQALTRDEVDMLRGVCTAMLAAARRARDQ"
     gene            complement(10169..11173)
                     /locus_tag="VGC69_10210"
     CDS             complement(10169..11173)
                     /locus_tag="VGC69_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295692.1"
                     /translation="MIPASLQRHPFVRKAAGLAAGLAGAFVLSSAQAAYPEQSVRLVV
                     GFPAGGTTDIVARVLSKELTTEFNQSFVVDNRPGAGSNLAASEVARAKPDGYTLLMVA
                     VTSAINDTLYKNLTFNLVKDFKAVALGAEVPNILVVNPKLPVQSVPELIAYAKANPGK
                     LAFASSGSGTSIHMAGELFKQKTGIDMMHIPYKGSGPAITDLIGGQVQLMFDNMPSSW
                     PSAQAGKLRALAVTTKERSPSAPDLPTLAELGIPELKDFDVSSWFGVIAPAGTPDDVV
                     NKLNAAIEKALHKPDVQARFKDLGAQIEYTTPQQFADMIKREVDTWRPVVKASGATVD
                     "
     gene            complement(11297..>11478)
                     /locus_tag="VGC69_10215"
     CDS             complement(11297..>11478)
                     /locus_tag="VGC69_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0295693.1"
                     /translation="SEYKDSGTLLNVRVWSQNANYWDLRWDLFHQIRTAMDAAGFKPP
                     AQVRDVREAGHQGAA"
BASE COUNT         1944 a         3869 c         3854 g         1811 t
ORIGIN      
        1 ggccgccagg gcgaaactgg cgtcggccgg cgagaaatcc agcgcggcca ggtcggcctg
       61 ggccacttcg gccagcacct cggccgcctc ggccatgtgg cgggcatggc gcgccacgat
      121 ggcctgccac ccgggccagc gcgcatcgag cgcgggcttg agctgcgtgc gcaccgccgc
      181 gcgggtgtag cgggcgtcgg tattggtggg gtcctcgacg ggctgccagc cctgggcccg
      241 ggcaaagtcc gccgccgcgt cacgcagcgc ggcgcgcggc acggccagcc aggggcgcag
      301 gtagcggatg ccgtcgcgct cggtttgcgc cgccatggcg gccatgccct gcaggccggc
      361 gccgcgcagc aggcgcagca gcacggtctc ggcctggtcg tcctggtgat gggccagcag
      421 gatgtggttt acgcccgcat gccgggccag gccggcaaag gcttcgtagc gggcctggcg
      481 ggccgcggcc tcgatgccct tgccggtatc gtccggcacg cgcacgcggg cctgcagcac
      541 gggggcgccc agcagcgcgc ccagggtctg gacgtgcgcg gcccagtcat cggcctgcgc
      601 cagcaggccg tggtggatat ggaacagggt gaggtcgatg cccaggccgc gtgccgcatg
      661 cgccgcggcc aaggccagca tggccgaatc ggccccgccg ctgacggcgg cggcgatgtg
      721 cgcggtgtcc ttgggcaagc cccgcagcgc agcgcgcacg caggcaatca ggtcggcggg
      781 acaatccggc aggcggcgct cggcatcggt catggagcgc tcgcgcggca ggtcgcccgc
      841 ccacgcaggc taggcgcgga cttcttggaa acggccgtag gacagcaggc gctgcacgcg
      901 ctgctcgacc agttcttccg gattcatgcc ctgcagctgg cgcagggtat cgcccagcgc
      961 gcggcgcagc aggcgcgcca tgacgcgcgg gtcgcgatgg gcgccgccca cgggctcgtt
     1021 gaccacgcgg tcgaccaggc ccaggtcttt caggcgcggg gccgtgatgg ccagggcttc
     1081 ggccgcgtcg ggcgccttct cggcgctgcg ccacagaatg gacgcgcagc cttcgggcga
     1141 gatcaccgag taggtggcgt attgcagcat gagcacggca ttgcccacgg cgatggccag
     1201 cgcgccgccc gagccgcctt cgccgatgat ggtgcagatg acgggcacct tgagctcggc
     1261 catggcgtac agattgtggc cgatggcctc ggactggccg cgctcctcgg cgccgatgcc
     1321 gggataggcg cccggggtat cgacgaaggt gaacacgggc agattgaact tctcggccag
     1381 gcgcatcagg cgcaaggcct tgcggtagcc ctcgggacgc ggcatgccga aattgcgcgc
     1441 ggcgcgctcc ttggtgtcgc ggcccttctg gtggccgatc accatgcacg cggcgccgtt
     1501 aaagcgcgcc aggccgccca cgatggactg gtcgtcggtg tacatgcggt cgccgtgcaa
     1561 ttcgtggaag tcggtgaaga tctcgcgcac gtagtccagc gtataggggc gctgggggtg
     1621 gcgcgccacc agggcggtct gccagggagt gagcttgccg tagatttcct tggccagcgt
     1681 ctggctcttt tgctgcagcc gtccgatctc gtcggaaatg tcgaccgccg agtcggcctg
     1741 cacgtagcgc agctgctcga tcttgttttc cagctcggcc aggggctgtt cgaattccag
     1801 gaatgtattg cgcatgaaga agattccggt aatggacgcc tgctttgttc ttgcccgggc
     1861 tcagtactgg accggtgtcg ggtccaggct gcgccagagg taccaggtcg ccacggtgcg
     1921 ccagggttgc catgccagcg aaacttcacg agcctcgaaa cgcgagaccg gttcgccact
     1981 gaaatagtgt aacgagattg ctttgagcaa ccccaggtca tccaacggca agatatccgg
     2041 ccgctgcaga ttgaagatca aaaacatctc cgctgtccag cggccgatac ccctaatagc
     2101 gaccagctcg tcaatgacgt cctcatcctg catggcggcc catttctcgg ggcgcacccg
     2161 gcgctcgctg aaatgcgcgg ccaggtcttg caggtattcg gccttgcgct gggacaggcc
     2221 ggcggcgcgc aggccttcca tgccggtttt cagcacgacc gccggcgccg ggcgcttgcc
     2281 cacggtgtcg atgaacttgc cccacacggc atcggcggcc cggacggaaa tctgctgtcc
     2341 gacaatggcg cgcgcaagcg taatgaaagg cgtgccgcgc gaggtcagcc agacctgcgg
     2401 gtgctgcggg atgatcttct tgagaatgcg gtcgcgcttt accaggtgcg cgacggcgtc
     2461 ttcccaataa tcgggcttct cgatggcgat atcagcgctg gacatggatg gacaactcgg
     2521 ttcaggcgcg acgccattgg gtgatgccgc ccggcttgtc gtcgagctcg atgccggcgg
     2581 catgcagctc ggcgcggatg cggtcggcct cggtgaaatt gcgggcctgc ttggccgcgg
     2641 cgcgggcggc gaccagggcg tcgatggcgg cggaatcgag ctcggccgag gcagccgagc
     2701 cggcacgata gcgcgtgacg ctctggaaat aggtctcggg atcctgctgc agcaggccca
     2761 gcaggccgcc caactgcttg agctgcccgg ccgcgcgcgc gcttttcgtg cggttggcct
     2821 cggatgccag ctcgaacagc gcggccacgg cgcccgaggt gttgaagtcg tcgtccatgg
     2881 cggccttgaa ggcctgggcc tggggctcgg tccagtcgat gttcagccag tcgggcgcga
     2941 cgttgcgcag ggcctggtag aggctgtcga gcgcgctctg ggcatcgagc agattgtcgg
     3001 gcgtgaagtt ctgcgggctg cggtagtgat tgcgcacgat gaaaaagcgc agcatctccg
     3061 cctcgcgcgc acgcacggcg taatcggcct ggctatcgga caggccggcc tcgtcggcca
     3121 ccgtctggcg gatggtgcgg aagttgccca gcgacttcga catcttgtcg gcgtcgacca
     3181 tgagcgggcc gcagtgcatc cacgtattgg ccatggtgcc gccgaaggcg ccctcggtct
     3241 gggcgatttc gttttcgtgg tgggggaact tcaggtcggg gccgccgccg tggatgtcca
     3301 ggggctggcc cagcagggcg tggctcatgg ccgagcactc gatgtgccag cccggccggc
     3361 cggggccgta gggagagtcc cacttggtgt cggccggctc ctcgggcttg gcggacttcc
     3421 acagcacgaa atccagcgga tcgcgcttgg ccgaacccac ggcgacgcgc tcgccggcgc
     3481 gcaagtcgtc gagcgtcttg cccgagagct tgccatagcc ggcaaagccg cgcaccgcat
     3541 agttcacgtc gccgtcatcg gcctggtagg ccaggccgtt ttcccgcagc ttggcgatga
     3601 tgccgagcat ttcgcccacg tactgcgtgg cgcgcggctc gtggtcgggc gactgcacgc
     3661 ccagcgcgag ctcgtcggcg tgcatggcgt cgatgtagta ggacgtcacc tcgccgatgc
     3721 gccggttggt ttccacggcg cggcggatga tcttgtcgtc gatatcggtg atgttgcgca
     3781 cgtattcgac ggcgtagccg ctggcgcgca gccagcgctg caccacgtcg aacgacacca
     3841 gcatgcgggc gtggcccagg tggcaatagt cgtacaccgt catgccgcag acgtacatgc
     3901 gcaccttgcc cgtttcaacc ggcacgaaag tcgtcttggc acgcgagagc gagttataga
     3961 tctgcagcat ggaacagggt tgttgcccgg caggaatgca ccccgaagat gcgaatcgga
     4021 atgccgggct aaaatggcgg tcgacaagta taacaagcgg cctgcctcgc aagcagcttc
     4081 cctggccggc cgctttccga cactatcatg cccatgtccg tgacctcgat gctgcgtttc
     4141 cgccgtgccg tactggcggc cgcctcgctg gccgtgctgg cggccgcccc gccggccgcc
     4201 caggcccaat ccatgggcgg cggcggcgcg ctcacgtcca acgccaacct caccacgctc
     4261 gacccctacc cgcccgagaa aggctggcag gggctgtccc atgcgctgga ccatctgcgc
     4321 cccggactcg atacccggct ccagccctcg ccctcgcaga tcaccgacca catcgaggcc
     4381 cggatcagca acggcgacta cgccggcgcc ctgagccaga tcgagcagcg cgaggcccag
     4441 ctcaagggca agcccggcac cgacgtgcag ctgctgttcc agcacgcgcg cgtgctggcc
     4501 gccctgggcc gcaccgacga agccatcgcg atctatacgg acatgaccac gcgctttccc
     4561 gaattgcccg aaccgtggaa caatctagcg gcactccacg ccaagcaagg caatctggac
     4621 caggcccgcg cagccctgcg catggcgctg cgggccgacc ccgactatgc cgatgcgcag
     4681 gcaaacctga agctgctggc ttcgcgcgat ccttccgcgg accagccccg gaccaaggaa
     4741 cccccttcgc aatgatgcat cgcaaccacc cgctgcgcct gtcggtctgc gcctttgcgc
     4801 tggccgccct gctccccctg ggagccggcg cccaaaccgg gtctactccc gactcttccg
     4861 ccaaaacaga taaaggcacg ctacacatga gcacacaacc ccgcgtcaaa atggaaacct
     4921 ccctgggtga cttcgtcctg accctggacg ccgagaaggc ccccaagacc accgaaaact
     4981 tccttaccta cgtcaaggaa ggcttctacg acggcacgat ctttcaccgc gtgatcgacg
     5041 gcttcatgat ccagggcggc ggcttcgagc cgggcctcaa gcaaaagccc acccactcgc
     5101 ccatcgagaa cgaggccaac aacggcctga agaacgacaa gtacacgatc gccatggccc
     5161 gcaccagcga tccgcactcg gccacggccc agttcttcat caacgcggcc aacaacgatt
     5221 tcctgaactt cacggcgccc acccccaatg gctggggcta caccgtgttc ggcaaggtca
     5281 ccgaaggcac cgagacgatc gacaagatca agggtgtgaa aaccggcaac agcggcttcc
     5341 accaaaacgt gccgaccgaa gatgtgctca tcaagaaagc cgaaatcgtt gaataggctg
     5401 gtgctgccgg aaggccccgt ctggctggcc tccgacctgc acttgagcga aagcaccccg
     5461 gccacgcagg aagcctttct gggctttttg caggcggccg ccggcgaggc cgcggcgctg
     5521 ctcctgccag gcgacatctt caatacctgg atcggcgacg acgtgatcac cgatccggag
     5581 ccctggctgg cccaggcgct ggacggcctg aagaccgccg ccgcgcgcct gccggtctac
     5641 ctgggccgcg gcaaccgcga ctttctcatg ggccgccgcc tggcccgcgc gctgggcgcc
     5701 cacctgcttc ccgacagtgt cctggcgaag accgcctacg gcgacgtgct gctgtcccac
     5761 ggcgacgaat actgtaccga cgatgaagcc taccagcgct ggcgccgcaa gtcgcgcaac
     5821 cccatcgtgc aacggatctt cctgctgcgc agcctggcct cgcgccagga cttcgcccgc
     5881 agggcccgcg aaaaaagcat ggcggccaag cgggacacgc cgccggccat catggacgtg
     5941 aacccgcagg ccgtggaaaa agccttcagg gaaagcggcg cgcgcctgat ggtgcatggc
     6001 cacacgcacc ggccggcccg ccacgtgctc acggtggacg gccggcagtg cgagcgctgg
     6061 gtgctgcccg actgggacct ggaccatgcg gagccgccgc gcggcggctg gctggtcata
     6121 gacgcggacg gcccgcagtt ctacgatctg gaatccgcct gaccggaacc gccatgtaca
     6181 cccccgccca cttcgacgaa agccgccccg aagccctgca cgacctcatc acccgccacc
     6241 ccctgggcat gctggtgacc cacggcaaga gcggcctgga cgccaaccac attcccttcg
     6301 agctggtgcc cggcgaaggg ccgcgcggga tcctgcgcgc ccacgtatcg cgcaacaacc
     6361 cggtgtggca ggacgtggcc aatggcgacg acgtgctggt ggtgttccgc gcggccgacg
     6421 cctacatttc gcccacctgg tatcccagca agcacgaggc gcacaagcag gtgccctcct
     6481 ggaactacat ggtggtccac gtccacggcc gcatcgccat ccgcgacgac gagcgctatg
     6541 tgcgcggcgt ggtggccagg ctgacgcgcg cccacgaggc cggccagccc gtcccctgga
     6601 agatgaccga cagcccggcg caattcatcg acgccatgct gcgcgccatc gtcggcatcg
     6661 aaatcgggat cacccgcttc gaaggcaagt tcaagctcag ccagaacaag gaagcgcgcg
     6721 acatccgcgg cgccggccaa gccctgaccc aagggggccc ggatgccgtg ggcgaggcca
     6781 tgctggcctg cgccgccgcc aaggccgcag cggaaaagta aaccgcgcta caccaggttt
     6841 tcgtgcgggt cgccgggttc ttcgggctcg atgtgcgact tctcgacccg caccgaggcg
     6901 atgcgccgtc cctctatctg cagcacttcg aagcggaagt gcatgtaatg cgcctcgtac
     6961 tcgcaggcat cgcccggctt gggcagatgg ccgaagcgcg ccagcagata gcccgccagc
     7021 gtggtgtagt cctgcgaatc gtcgaccagg ccgtcggtct cgagcacctg ttcgacatga
     7081 tgcaggtcgg ccgcgccgtc gatgcgccag acattcgcgc gttcgaccag gatgtcgggc
     7141 atttcgtctt cgtcggggaa ctccccggca atggcctcga acacgtcgat gggcgtgacc
     7201 aggccgacga tggcgccgaa ttcgtcggcg atcagcacca attgcccgcg cgagcgcttg
     7261 agggtgtcca tcaagcgcag gatgccgatg gcctcgtgca cgatgatcgg gtcgcgcagg
     7321 tggctatggt cgatgtggcc ctcggtgatg agatcggcca ccaggtcctt ggcgcggcct
     7381 atgccgatga tttcgtcgag cgcgccgcgg cacaccggga aaaagctgtg cggggcggcg
     7441 gagatctgct cgcggatggc tgccgcgtca tcctcgatat tgatccatga cacatcggtg
     7501 cgcggcgtca tgatggagtg gatgctgcgc tcggccaggg tcagcacgcc gctgaccata
     7561 ttgcgctctt ccacgccaaa ggccttctgc gcggcctggg cagacggcgg cacatcgaca
     7621 tcgggctcga cgtcgagcgg gcgctggccc agcatgcgca tcacggtttc ggccgtgcgc
     7681 tcgcgcatgg gccgcacgcc gtcgagccgc agcaggttgc gccgcgccac ctggttgagc
     7741 gattcgatgg cggccgaaaa agcgatggcc gcgtacagat agcccttggg aaccttgaag
     7801 cccaggctct cggccaggag cgagaagccg atcatgagca ggaagcccag gcacagcacc
     7861 accacggtgg gatgcgcatt gacaaagcgc gtgagcggct tggaggccac cagcatgatg
     7921 ccgacggcga tcaccaccgc cgtcatcatg atggccaggt ggtcgaccat gccgaccgcg
     7981 gtgatcaccg aatcgatcga gaagatcgcg tcgagcacca cgatctgcgt cacgatgacc
     8041 cagaagctgg cgtagatgcg cttgcccagg ccgtcggcat ggatgctgcc ttccaggcgc
     8101 tcgtgcagct cgaccgtgcc cttgaagagc aggaacaggc cgcccaccag caggatgagg
     8161 tcgcggcccg agaaagacag ggggccgagg gagaacagcg ggcgggtcag cgtgaccagc
     8221 caggacatga ccgtcagcag caccagccgg gtgaccaggg ccagccccag gcccatgatg
     8281 cgagccctgt cgcgctgctt gggcggcagc ttgtcggcca ggatggcgat gaaaatgagg
     8341 ttgtcgatac ccaggacgat ttcgaggacg accagggtga gcaggccgac ccacgccgtc
     8401 gggtccagca accatgccat cagggactcc agcagttcac aagcggccta tcgtacagga
     8461 atcaggttgc aggtttgttc tgcaggcccg ccaccatctg cgcgaagatc ttgggggtgc
     8521 cggccagcac gtcgccggaa tccagccagc tctgttcgcc gtcgaagtcg gaaaccaatc
     8581 cgcccgcctc gagcaccagc aggctgccgg cggccaggtc ccagggcttg aggcccacgc
     8641 cgcagaaacc gtccagccgc cccgcggcca cgtaggccag atccagcacg gtggcgccca
     8701 tgcgacgcac gctggagcag ccttcggcca tgccggcaaa gcgcggcgcg cccaggcccg
     8761 gattggtcga acccggccag tgggcgccca acagcgcctc ggcgaagcgg atgcggcccg
     8821 agacgcgcac gcggcggtcg ttcaggaagg tgccgccgcc gcggctggcg gtgaaaagct
     8881 cgttgcgcga cgggtcgtac acgacggcct gggtgatctg cccgcgatgc tgcagggcga
     8941 tggacaccgc gtaggtgggc aggccatgga tgaaattggt ggtgccgtcc agcggatcga
     9001 tgatccatag aaactcggaa tgttccgacc cctggcggcc gtattcctcg cccagcacgg
     9061 catggtcggg gtaggccgcg agcagcgctt cgacgatggc ttcttccgag gcgcggtcga
     9121 cttccgtgac ataatcacgc ggccccttgc gcgccacgct gaggcgatcc agatcgaggc
     9181 tggcgcgatt gatgatggtg ccggcacgcc gggccgcctt gatggcggtg ttgagcatcg
     9241 gatgcatagg gttccgtacg agtgaagagc aaaaaggcga ccgcggcatt gcaggcaatg
     9301 cgcggacccg ccaaaccacc tattttaatt taaatgaccc aggcattttc gcgggtgcgt
     9361 ttcatcatga cgcaacccag tcacccaggc aatgtcggct cggccgcccg ggccatcaag
     9421 accatggggt tttccgagct ggtgctggtc gagcccaagc tgccggacat ggccgcccag
     9481 cccgaggccg tggccctggc cagcggcgcc gtcgacgtgc tggaacgcag ccaggtcttc
     9541 gacacgctgg aagaggcgct ggcccccgcc accctggctt tcgccctgac ggcccgggtg
     9601 cgcgacctgg gcccgccgcc ctgcgacatc cgccagacgg ccgagctggc ccgcgcccac
     9661 ctggccggga ccggccaggg ctgcgtggcc attgtgctcg gcaccgagcg cgccggcctc
     9721 accaacgccc agatcgccct gtgccaccgc atctgccaca ttccggccaa tccggaatac
     9781 agctcgctca acgtggccca ggccctgcag ctggccgcct gggaaatgcg ttacgccttg
     9841 ttgcaagaaa ccggtgcccc gttgctgccc gcctcgaccg cgcagcagcc cgaccccggc
     9901 gcggcccccg ccgacggcgc ggccgtgcag gccctgctgg cccactggga agaagccctg
     9961 gtggccgtgc agttcctcga tcccgcccat cccaagaagc tcatgccgcg catgcggcac
    10021 ctcttcagcc gccaggcgct gacccgggac gaagtcgaca tgctgcgcgg cgtgtgcacc
    10081 gccatgctgg ccgcggcccg gcgcgcgcgg gaccaatgaa aacagccctt ccctgcgagg
    10141 cgagccgccg tgtacgcggc aagcgggttc agtcgaccgt ggcgccggaa gccttgacga
    10201 ccgggcgcca ggtatcgacc tcgcgcttga tcatgtcggc gaactgctgc ggcgtggtgt
    10261 attcgatctg ggcgcccagg tctttgaagc gcgcctgcac atcgggcttg tgcagcgcct
    10321 tctcgatagc ggcgttgagc ttgttcacca cgtcgtcggg cgtacccgcc ggggcgatca
    10381 cgccgaacca ggacgacaca tcgaagtcct tgagctcggg aatgcccagt tcggccagcg
    10441 tcggcaggtc gggcgcgctg ggcgaacgct ccttggtcgt caccgccagc gcgcgcagct
    10501 tgccggcctg cgcggaaggc caggacgacg gcatgttgtc gaacatcagc tgcacctggc
    10561 cgccgatcag gtcggtgatg gcggggcccg agcccttgta ggggatatgc atcatgtcga
    10621 tgccggtctt ctgcttgaag agctcgcccg ccatgtggat ggaagtgccg ctgcccgacg
    10681 aggcgaaggc cagcttgccc gggttggcct tggcgtaggc gatcagttcc ggcacggact
    10741 ggaccggcaa tttgggattg accaccagga tattgggcac ttcggcgccc agggccacgg
    10801 ccttgaagtc cttgaccagg ttgaaggtca gattcttgta cagcgtatcg ttgatggcgc
    10861 tggtgacagc caccatcagc agggtatagc catcgggttt ggcgcgcgcc acctcggaag
    10921 cggccaggtt gctgcccgcg ccgggccggt tgtccaccac gaaggactgg ttgaattcgg
    10981 tggtgagttc cttggacagg acgcgggcca cgatgtcggt cgtgccgccg gccgggaaac
    11041 cgaccaccag gcgcaccgac tgctcgggat aggcggcctg ggcgctggat agaacaaagg
    11101 cgcccgccag gcccgcggcc agtccggcgg ccttgcgaac gaagggatgg cgctgcagtg
    11161 aagcaggaat catgggcttg tctccggttt ggcgtccatg cgcccggctc gggcgagccg
    11221 gcgcgtggac ttatttattg gtttgcgggg ctgatgttat caccggaccg ccccgctgca
    11281 agcctcagga aatgccctag gccgcgccct gatggccggc ctcgcgcacg tcgcgcacct
    11341 gggccggcgg cttgaagccc gccgcgtcca tggccgtgcg gatctggtgg aacaggtccc
    11401 agcgcaggtc ccaataattc gcgttctggc tccagacccg cacgttgagc agcgtgccgc
    11461 tgtccttgta ctcgctgg
//
LOCUS       DASWPO010000194         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2902373, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000194 DASWPO010000000
VERSION     DASWPO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2902373"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VGC69_10220"
     CDS             <1..651
                     /locus_tag="VGC69_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295694.1"
                     /translation="HKGINRRAARRQAIGLLDLVGIPAAAQRVDNYPHQMSGGMRQRV
                     MIAVGLACRPSLLIADEPTTALDVTVQAQILQLLRDLQRELSMSVLLITHDLGIVTEF
                     ADRVTVLYAGRVAERAPVAPLFTAPAHPYTQGLLASIPPLDRDVDQLTPIPGAVPGLA
                     DMPDGCRFAPRCGLALPACEAARPPLHALDAQHQAACIRLAETLKNGPIAAYRPEP"
     gene            652..1668
                     /locus_tag="VGC69_10225"
     CDS             652..1668
                     /locus_tag="VGC69_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0295695.1"
                     /translation="MPESLPHPAAARPAEPLVSVRMLSKHFGGQAGLFSRRDAPVVRA
                     VDGVSFDIARGETLGVVGESGCGKSTTGRLLIRMLDATGGHVLYEGVDLLQLDRQRMR
                     EYRRKVSIVFQDPFASLNPRMTVGSIIAEPMDIHGLYRGGERRRRLLEIMDRVGLPAA
                     YLQRHPHEFSGGQRQRIGIGRALALDPDFVVCDEAVSALDVSIQAQIINLLDALQRER
                     GLTYFFISHNLAVVRHIADRVAVMYLGSVVEIAPTRELYANPRHPYTHALLSSIPVPV
                     VGAARERIELQGDLPSPLDPPSGCRFHTRCPLAKAECKSDDPPLRKVADAQGGAHYVA
                     CHFA"
     gene            complement(1684..2712)
                     /locus_tag="VGC69_10230"
     CDS             complement(1684..2712)
                     /locus_tag="VGC69_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0295696.1"
                     /translation="MPTSTRSRTAASGDAVQKNGPTQKDVARMAGVSQSAVSRVFAGR
                     GYVAAEVRREIERAARKLGYVPDMAARSLVSGRSNIVAVVTSNITHPFVPQMLEKLTL
                     AIQRRGQEVLLLNSPPGQDIDRQVPIAMRYRVRGILIANVSLGTAIIKRVRASGTPIV
                     MMNRYVEEKAVHAVSCDNAHGSRLIADALVAAGHRRIAYIGGLSASPTNAVRKQGFLK
                     ALKAHGLAPALALDGEFTHAWGYAAVDLLAAQKPPVDAVLCGDDMVALGMLDGLRERH
                     DRFKCMPAVVGFDDIPNASWSPYMLTTYSNPLDDMVEAALSLLELPADAAPMRKVMEG
                     ALVRRKSF"
     gene            complement(2766..3812)
                     /locus_tag="VGC69_10235"
     CDS             complement(2766..3812)
                     /locus_tag="VGC69_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0295697.1"
                     /translation="MPAQSASGPGAPRAGRLHQPVKRVADQRIRVEAPEGAGELPVYA
                     NRPIDAPAPGIRQAFIIVHGTLRNADDYYAIGLKVLAQAGEDGRHSLLVAPQFLTEPD
                     VRAHSLPGQVLAWSQNGWKDGAAARRPAPISSFSAFDALLAHFADRRLYPDLSSVIVV
                     GHSAGAQAVQRYAVAGQGEAALTRAGIAVRYVVANPSSYAYFDEQRPAPAGGFAPADA
                     GACPSAVQWKYGMAMAPPYVSAQDGGTIEARYAARKVTYLLGMNDTDPRRHFIDRSCA
                     AMAQGPYRLARGLAYFDYVRRRHPSGLRQSLVEVPGVGHDGAGMLTSACGMAVLFGRK
                     PPASCPATLETGLE"
     gene            complement(3969..>4726)
                     /locus_tag="VGC69_10240"
     CDS             complement(3969..>4726)
                     /locus_tag="VGC69_10240"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041034.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEY0295698.1"
                     /translation="IPHGGGGPGVGPVAVREHLAPYLPGVLDTQGKLPAGAKTGPVAA
                     APFGSAGILPIPYVYVVLMGAEGLKRATEVAILNANYIASRLNAYYPVLYSGRNGRVA
                     HECILDLRPLKDATGISGEDVAKRLMDYGFHAPTMSFPVAGTLMVEPTESEGLVELDR
                     FIDAMIAIREEIAQVERGERDAEDNVLKNAPHTAQMLLAEEWHHDYPRQQAAYPVASL
                     RDNKYWPPVARVDNVYGDRNLVCSCPPIEAYAS"
BASE COUNT          707 a         1712 c         1600 g          707 t
ORIGIN      
        1 cacaagggca tcaaccgccg cgcggcgcgc cgccaggcca tcggcttgct ggacctggtg
       61 ggcatccccg ccgcggccca gcgggtcgac aactatccgc accagatgtc gggcggcatg
      121 cgccagcgcg tgatgatcgc cgtgggcctg gcctgccggc ccagcctgct catcgccgac
      181 gagcccacca cggccctgga cgtgacggtc caggcgcaga tcctccaact gctgcgcgac
      241 ctgcagcgcg agctgtccat gtcggtgctg ctgatcaccc acgacctggg catcgtgacc
      301 gagttcgccg accgcgtgac cgtgctgtat gccggccgcg tggccgagcg cgcgccggtg
      361 gcgcccttgt tcaccgcccc cgcgcatccc tatacgcaag gcctgctggc ctcgatcccg
      421 ccgctcgacc gcgacgtgga ccagctcacg cccattccgg gcgcggtgcc cggcctggcc
      481 gacatgcccg acggctgccg cttcgcgccg cgctgcggcc tggcgctgcc ggcctgcgaa
      541 gccgccaggc cgccgctgca tgcgctcgac gcgcagcacc aggccgcctg catccggctg
      601 gccgaaaccc tgaagaacgg gccgatcgcc gcctatcgcc cggagccctg aatgccagag
      661 tcccttcccc accccgctgc tgcccggcct gccgaacccc tggtctcggt gcgcatgctg
      721 agcaagcatt tcggcggcca ggccggcctg ttctcgcgcc gcgacgcgcc ggtggtgcgc
      781 gccgtggacg gcgtcagctt cgacatcgcg cgcggcgaaa ccctgggcgt ggtgggcgag
      841 tccggctgcg gcaagtccac cacgggccgg ctgctgatcc gcatgctcga cgccaccggc
      901 ggccacgtcc tctacgaagg cgtcgacctg ctgcagctcg accgccagcg catgcgcgag
      961 taccgccgca aggtctccat cgtcttccag gatccgttcg cctcgctgaa cccgcgcatg
     1021 accgtgggct ccatcatcgc cgagcccatg gacatccacg gcctgtaccg cggcggcgaa
     1081 cgccggcgcc gcctgctgga gatcatggac cgggtcggcc tgccggccgc ctacctgcag
     1141 cgccatccgc acgaattctc cggcggccag cgccagcgca tcggcatcgg ccgggccctg
     1201 gcgctggatc cggatttcgt ggtgtgcgac gaggccgtct cggcgctgga cgtctcgatc
     1261 caggcgcaga tcatcaacct gctcgatgcg ctgcagcgcg agcgcggcct gacgtacttc
     1321 ttcatctcgc acaacctggc ggtggtgcgc cacatcgccg accgggtcgc ggtcatgtac
     1381 ctgggcagcg tggtcgagat cgcccccacg cgcgagctct acgccaaccc caggcatccc
     1441 tacacgcacg ccctgctgtc atccatcccc gtgccggtgg tgggcgccgc gcgcgagcgc
     1501 atcgaactgc agggcgacct gcccagcccg ctcgatccgc cttcgggctg ccgcttccat
     1561 acgcgctgcc ccctggccaa ggccgaatgc aaaagcgacg acccgccctt gcgcaaggtg
     1621 gccgatgcgc agggcggcgc gcactacgtc gcctgccatt tcgcctgagc ggcaagcgcg
     1681 gcgctagaag gatttgcgcc gcaccagcgc gccttccatg accttgcgca tgggcgcggc
     1741 gtcggccggc agctccagca gggacagcgc ggcctcgacc atgtcgtcca gcggattgct
     1801 gtaggtggtc agcatgtagg gcgaccagct ggcgttggga atatcgtcga agcccacgac
     1861 ggcaggcatg cacttgaacc ggtcgtggcg ctcgcgcaag ccatcgagca tgcccagcgc
     1921 caccatgtcg tcgccgcaca gtaccgcatc cacgggcggc ttctgcgcgg ccagcagatc
     1981 gactgccgca tagccccagg catgggtgaa ctcgccgtcg agcgccagcg cgggcgccag
     2041 gccatgcgcc ttcagcgcct tcaggaaacc ctgcttgcgc accgcgttgg tgggcgaggc
     2101 ggacagcccg ccgatgtagg caatgcgccg gtgcccggcg gcgaccagcg catcggcgat
     2161 cagccggctg ccgtgcgcgt tgtcgcacga cacggcatgc acggcctttt cctcgacgta
     2221 gcggttcatc atgacgatgg gggtgccgct tgcccgcacg cgcttgatga tggccgtgcc
     2281 gagactgaca ttggcgatca ggatgccccg cacgcggtag cgcatggcaa tgggcacctg
     2341 cctgtcgatg tcctgccccg gcggggaatt cagcagcagc acctcctggc cgcgccgctg
     2401 gatggccagc gtcagcttct cgagcatctg cggcacgaaa gggtgggtga tgttgctggt
     2461 gaccaccgcg acgatgttcg accggccgct gaccagcgaa cgcgccgcca tgtcgggcac
     2521 atagcccagc ttgcgcgcgg cgcgctcgat ctcccggcgc acctccgcgg ccacatagcc
     2581 acggccggcg aacacgcgcg acaccgcgct ctgcgacacg ccggccatgc gcgccacgtc
     2641 tttctgggta ggcccgtttt tctgcacggc atcgccggaa gcagcggtgc gggagcgcgt
     2701 cgaggtcggc aagatagttt tagtcgggaa agaaagtcat gggcggcgag gcggcggcag
     2761 gcgggtcact ccaggccggt ttcaagtgta gcggggcatg aggccggcgg ttttctgcca
     2821 aacagcaccg ccatgccgca tgccgaggta agcatgccgg cgccatcatg ccccacgccc
     2881 ggcacctcga ccaggctctg ccgcaggccg gacggatggc gccggcgcac ataatcgaaa
     2941 tacgccagcc cgcgagccag ccggtacggc ccctgggcca tggccgcgca tgagcggtcg
     3001 atgaaatggc ggcgggggtc ggtgtcgttc atgcccaata gataggtcac cttgcgcgcc
     3061 gcatagcggg cctcgatcgt gccgccgtcc tgcgccgaaa catacggcgg cgccatggcc
     3121 atgccgtact tccattgcac ggcggacggg cacgcgccgg catcggcggg ggcaaacccg
     3181 ccggccggcg ccggccgctg ctcgtcgaag tacgcatagc tcgacggatt ggccaccaca
     3241 tagcggacgg caatgccggc gcgcgtcagc gcggcctcgc cctggccggc caccgcatag
     3301 cgctgcacgg cctgtgcgcc ggcggaatgg ccgaccacga tcacgctcga cagatcggga
     3361 tacaggcgcc ggtcggcgaa atgcgccagc agggcatcga atgccgagaa tgaactgatg
     3421 ggcgcggggc gccgcgccgc cgcgccgtct ttccagccgt tctggctcca cgccagcacc
     3481 tgcccgggca gggagtgggc ccgcacgtcc ggctcggtca ggaattgcgg cgcgacgagc
     3541 aggctgtgcc ggccgtcctc gccggcttgc gccagcacct tgaggccgat tgcatagtag
     3601 tcgtcggcat tgcgcagcgt gccgtggacg atgatgaacg cctgccggat ccccggcgcc
     3661 ggggcatcga tgggccggtt ggcatagacc ggcagttcgc ccgcgccttc gggcgcctcg
     3721 acccggatgc gctggtcggc gacacgcttg accggctggt gcagccgccc cgcgcgcggg
     3781 gcgcccggcc cgcttgccga ttgcgcgggc atgccatgcg cggcagcgcc cgccgccagc
     3841 gcaatgccca ccgcgatgcg caatgcgcgc cagaattcct tcatgcccag cccccttcct
     3901 gacatcgatc ccccgcccca tgaaaaaagg cggctcatcg ccgccctttt tatcaccaaa
     3961 gccatgcgtc aggacgcata tgcctcgatc ggcgggcacg agcacaccag gttgcggtcg
     4021 ccgtagacat tgtcgacgcg cgccacgggc ggccagtact tgttgtcgcg caaggacgcc
     4081 accgggtagg cggcctgctg gcgcgggtag tcgtggtgcc actcttcggc cagcagcatc
     4141 tgggcggtgt gcggggcatt cttgagcaca ttgtcctcgg catcgcgctc gccgcgctcg
     4201 acctgggcga tttcctcgcg gatggcgatc atcgcgtcga tgaagcggtc gagctcgacc
     4261 aggccttccg actcggtggg ctcgaccatc agcgtgcccg ccacggggaa gctcatggtg
     4321 ggggcgtgaa agccatagtc catcaaacgc ttggccacgt cctcgcccga gatgccggtg
     4381 gcgtccttga gcggacgcag gtcgaggatg cactcgtgcg ccacgcggcc gttgcgcccg
     4441 gagtacagca ccgggtagta cgcgttcagg cgcgaggcga tgtagttggc gttgaggatg
     4501 gccacctcgg tcgcgcgctt gaggccttcg gcgcccatca ggaccacgta cacataggga
     4561 atcggcagga tgccggccga gccgaagggc gccgccgcca ccgggccggt cttggcgccg
     4621 gcgggcagct tgccctgggt gtccagcacg ccgggcaggt agggcgccaa gtgctcgcgc
     4681 accgccacgg ggcccacgcc cgggccgccg ccgccgtgcg ggatgc
//
LOCUS       DASWPO010000195        10298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2276220, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000195 DASWPO010000000
VERSION     DASWPO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276220"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1006
                     /locus_tag="VGC69_10245"
     CDS             143..1006
                     /locus_tag="VGC69_10245"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY0295699.1"
                     /translation="MNDDLRPARWRTLWLSDIHLGTSGCKAQFLLDFLEHNDADTLYL
                     VGDIIDGWQLRKHWYWPQQHNDVVQRILRKARNGTRVIFVPGNHDEFAREFIGYAFGD
                     IEILDEDVHVTAKGLKLLVLHGDQFDGVIQHSKWLAHLGDTLYQFTLWLNHHFNRLRH
                     RMGLHYWSLSQYLKHKVKNAVSFITHFEEALAGEARRRGLDGVVCGHIHKAELREVDG
                     ILYCNDGDWVESLSALAESQDGQLQLLDWAAVLAQREGSKTPRSNRPMSLPALPSSLR
                     PVQQVTPAKSR"
     gene            1163..2821
                     /locus_tag="VGC69_10250"
     CDS             1163..2821
                     /locus_tag="VGC69_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0295700.1"
                     /translation="MNDHYQALYQTFHWLVPAQFNIAQVCCHQWAASAPQARRIAIYD
                     EDEAGKREVWTFGRLAEATNQLANGLIRMGVSPGDRVAVAMGQRPETVVAHMAIYSVG
                     AVSVPLSALFGPEAMETRLRDSGAHVALIDTASSAALLGVADRCPALTQIVGVGLADE
                     RVLPWRSLLARQSANFKPVATRSSDPAILLYTSGTTGAPKGALLPHSVLIGNLPGFVA
                     SQDWFPRPADVFWSPADWAWTGGLMDALLPTLYFGRPIVGTRGRFSPERAFELMERYQ
                     VTNTFLFPTALKMMMKSVPDPRERYKLALRAVMSAGESVGETVHAWCESALGLTPNEM
                     FGQTEMNYVVGNSSKRWPSRPGSMGRPYPGHNVAVIDEQGQVCPAGEIGEVAVNRYDI
                     HGHPDPVMFLGYWHNEQATRDKFSGDWCRTGDLANADADGYLWYAGRADDVFKSAGYR
                     IGPGEIENCLLGHPAVGNAAVVPKPDAERGALVKAYVVLTPAYQDRPRDEITQALQEH
                     VRDRLAPYEYPKEIEYVEELPMTTTGKIQRNVLRKRELELASGR"
     gene            2947..3468
                     /locus_tag="VGC69_10255"
     CDS             2947..3468
                     /locus_tag="VGC69_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY0295701.1"
                     /translation="MAIYQLDDFVPDIHPSAYVADSADIIGQVTLREGASIWSQVSIR
                     GDNEPITIGRHSNVQESSVLHVDAGCPLVVGEHVTVGHQAMLHGCTIEDGALIGMQAI
                     VLNRAVVGRNCLVGAGAIIPEGRVIPDNSLVIGIGKVVRELSAEEIATMHANTRHYVE
                     RGQQYKTALKRIG"
     gene            complement(3557..5542)
                     /locus_tag="VGC69_10260"
     CDS             complement(3557..5542)
                     /locus_tag="VGC69_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0295702.1"
                     /translation="MPGLSTFLQDLVPLVDQVIDGFHRSLRVLPKSQNLLKALSADDL
                     DKLKRWEAANLLKLLAPDLTAKGHETMARAIGRVHVLSCVTKEELSFSKDLLYAAVCS
                     HVDQDKHSQALSVLSARMMRDLAWHLTVFQEIYAQRVQLLLNVTRLSWEAINYTDLIA
                     KTAVLLAAHPGICACFFGRPSSEETFNFEVLEGPRLESYLTRVQEIRTGYFNAGSPWS
                     GRSPADKAWRSNRIEHCINIATDPDMPPWRDVALAHRFRSIVAVPLVGQPDIGPAVVL
                     GLYSDHTGGFSSDDQTAFLEQLQSLLTYAIIRLEAQEGGTQTVSYGVRRRWAALLKSD
                     ALCMHYQPLLDLKTRRVTKVEALARLRDGDRLLSPNEFFPALTSEDFFVLYAQGLEQA
                     LRQRAVWQRSGMDLGISVNMPVDALGDVRYYNTTVQALLAHGCPASLLTLEILETNEM
                     PVGVDVATELGRYKELGVGLAEDDLGSGHSSLMRLRELPFDTVKIDRSLVMLNGENSA
                     DVLHFTYQLTRLGHSLGKSVVVEGVENADLLEAVAILGADAIQGYVLTRPMPADQITA
                     WLSEHLVYPAPAHTQSVLAQMARLLIWEECLYLLRDEATTTGGIADINTSVLPFGAID
                     AGIQRVFVLTAVNQGPGSPEYRQAREQLMQALIAKTA"
     gene            complement(5726..6448)
                     /locus_tag="VGC69_10265"
     CDS             complement(5726..6448)
                     /locus_tag="VGC69_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEY0295703.1"
                     /translation="MRRVLFPFQHGSALVESAVALGVVLFAGLLALEFAHWQLMRHLA
                     QVALTEAARAGATGHGRPSLMAQAFEAGMAPRYAAPDGGSRQRLRAAWARTAARTGLP
                     AWRIWVLQPDAAAHADFGQTGLPLPDDSRGQPAIRNDYQAEQHARYAARWPQGRGPRS
                     GLTIFEANMLHLRLLYLVDPLVPALRPLLRSLASGATDACTRQALAAGALPLRLDLLM
                     DMQSHPVNWPAGHGVLVSDYMC"
     gene            complement(6561..7484)
                     /gene="hslO"
                     /locus_tag="VGC69_10270"
     CDS             complement(6561..7484)
                     /gene="hslO"
                     /locus_tag="VGC69_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY0295704.1"
                     /translation="MTDQLKKYLFEDRTARVQAVRLTDTWREARANHQYPPAVCKLLG
                     ELVAASTLLASNIKFDGSLLLQIQGDGPIALLVVECRADLSLRATVKVREGHTVPETG
                     TLQSLLNTHGDGRFIVLLDPRRKVPGQQPYQGIVPLEGETVAEVLEHYMKSSEQLDTR
                     MWLAADDHHAVGLLVQRLPDQGGTPGEAASGSDTWNRAVQLAATLGEAELLAADVDTL
                     IRRLYWEETLVAFEPETVRWHCPCGRERVADMLRMLGQSEIRSILAEQGKVEVSCDFC
                     GKPYLFDAVDCAGLFAGGTQVADRQDPPTVH"
     gene            complement(7506..7850)
                     /gene="ftsB"
                     /locus_tag="VGC69_10275"
     CDS             complement(7506..7850)
                     /gene="ftsB"
                     /locus_tag="VGC69_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEY0295705.1"
                     /translation="MRLLFLVLLLLAGLIQYPLWLGKGGWLKVWDMQKQVATQHETND
                     SLRARNAALQAEVKNLESGTEAVEERARGELGMMREGEVFVNILPPGAAVPNTPPVPA
                     AKPAPPRPQPRR"
     gene            complement(7874..9160)
                     /gene="eno"
                     /locus_tag="VGC69_10280"
     CDS             complement(7874..9160)
                     /gene="eno"
                     /locus_tag="VGC69_10280"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY0295706.1"
                     /translation="MSAIVDIIGREILDSRGNPTVECDVLLESGAMGRASVPSGASTG
                     AREAIELRDGDKDRYLGKGVLRAVENLNTEISEALMGLDAQEQTFVDRTLIELDGTES
                     KERLGANAILAASMAVARAAADESGLSLYRYFGGSGPLSMPVPMMNVINGGAHANNTL
                     DMQEFMILPVGAASFREALRWGSEVFHSLKKLINAQGMSTAVGDEGGFAPNVANHEAA
                     IQLILKAIGEAGYEPGTQIALGLDCASSEFYKNGKYVLEGEGGVSLSSQEFTNLLAAW
                     CDKYPIISIEDGMAENDWDGWKLLTDQLGKKVQLVGDDLFVTNTKILREGIRKGVANS
                     ILIKINQIGTLTETFAAIEMAKRAGYTAVVSHRSGETEDSTIADIAVATNAMQIKTGS
                     LSRSDRMAKYNQLLRIEEELAEVASYPGIEAFYNIR"
     gene            complement(9200..10072)
                     /gene="kdsA"
                     /locus_tag="VGC69_10285"
     CDS             complement(9200..10072)
                     /gene="kdsA"
                     /locus_tag="VGC69_10285"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY0295707.1"
                     /translation="MNKLCGFDVGLDKPFFLIAGPCVIESRQMAFDTAGQLKEIAGKL
                     GIPFIYKSSFDKANRSSGKSYRGPGMDEGLKILADVKKELGVPVLTDVHDTPQIEPVA
                     SVVDVLQTPAFLCRQTDFIRACAATLKPVNIKKGQFLAPHDMVQVVNKARDAAIEAGG
                     DGGTIMVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTSSGGQR
                     EFVPVLARAAVAVGVAGLFMETHPDPAHAMSDGPNAVPLGQMAALLETLADLDRITKR
                     NGFIEDRADWACAG"
     gene            complement(10136..>10298)
                     /locus_tag="VGC69_10290"
     CDS             complement(10136..>10298)
                     /locus_tag="VGC69_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295708.1"
                     /translation="EHLPEMMELPSHPWFVGVQFHPEFTSTPRDGHPLFSSFIRAALE
                     HKARRVKGD"
BASE COUNT         1757 a         3416 c         3430 g         1695 t
ORIGIN      
        1 gccctccgcc ccgcactccg tttcttgctc aggccgcgcg cagcgggacc gcaacagtca
       61 caaagccgtc attcaaaagt cacgaatgcg tcacccctaa atggtctcat gctggagcaa
      121 caaggcctga acgggaaacg ctgtgaacga cgacctccgc cctgcgcgct ggcgcactct
      181 ctggctctcg gacatccatc tgggaacatc cggctgcaag gcccagttcc tgctggactt
      241 tctcgaacac aacgatgccg acacgctcta cctggtcggc gacatcatcg acggctggca
      301 gttgcgcaag cactggtact ggccgcagca gcacaatgac gtggtgcagc gcatcctgcg
      361 caaggcccgc aacggcaccc gcgtgatctt cgtgccgggc aaccacgacg agttcgcccg
      421 cgaattcatc ggctatgcct tcggcgacat cgagatcctc gacgaggacg tgcacgtcac
      481 cgccaagggc ctgaagctgc tggtgctgca cggcgaccag ttcgacggcg tgatccagca
      541 cagcaagtgg ctggcccatc tgggcgacac gctctaccag ttcaccctgt ggctcaacca
      601 ccacttcaac cgcctgcgcc accgcatggg cctgcactat tggtccttgt cgcagtacct
      661 caagcacaag gtcaagaacg ccgtctcctt catcacgcat ttcgaggaag ccctggcggg
      721 cgaggcccgc cgccgcggac tggacggcgt ggtgtgcggc cacatccaca aggccgaact
      781 gcgcgaggtg gacggcatcc tgtactgcaa cgacggcgac tgggtggaaa gcctgtcggc
      841 cctggccgag tcccaggacg gccagttgca gctgctggac tgggccgccg tgctggccca
      901 gcgcgagggc tcgaagaccc cgcgctcgaa ccgcccgatg tcgctgcctg ccctgccctc
      961 gtctttgcgc ccggtacaac aggtcacgcc ggccaaatcc cgctaaaacc ccagtaataa
     1021 cccttatatg tccctaatag gggcctggac atattgcagt ccgggtcccg ctaggtcatg
     1081 cttgcgtctg ggcgtgcccg gcacactgcc accgctggcg ggccggttcc tgaacccgtg
     1141 tgcgcttaca caccctaggg ccatgaacga ccactaccag gcgctctacc aaaccttcca
     1201 ctggctggtt cccgctcaat tcaatattgc gcaggtctgt tgccaccaat gggccgccag
     1261 cgcgccgcaa gcgcgccgca tcgccatcta tgacgaagac gaggccggca aacgggaagt
     1321 ctggaccttc ggccgattgg ccgaggccac caaccaattg gccaacgggc tgatccgcat
     1381 gggcgtgtcc ccgggcgacc gggtcgcggt ggccatgggc cagcggcccg agaccgtggt
     1441 ggcgcacatg gccatctaca gcgtcggagc cgtgtccgtg ccgctttccg cgctgttcgg
     1501 cccagaagcc atggaaaccc gcctgcgcga ctcgggcgcc cacgtggcgc tgatcgacac
     1561 cgcctccagc gccgccctgc tcggcgtggc cgaccgctgt cccgcgctca cgcagatcgt
     1621 cggcgtcggc ctggccgacg aacgcgtgct gccctggcgc agcctgctgg cgcgccagtc
     1681 cgccaacttc aagcccgtgg ccacccggtc gagcgacccg gccatcctgc tctatacctc
     1741 gggcaccacc ggcgcgccca agggcgccct gctgccgcac agcgtgctga tcggcaacct
     1801 gcccggcttc gtcgcctcgc aggactggtt ccccaggccg gccgacgtgt tctggtcgcc
     1861 ggccgactgg gcctggaccg gcggcctgat ggacgccctg ctgcccaccc tgtacttcgg
     1921 ccgccccatc gtcggcacgc gcgggcgctt ctcgccagaa cgcgccttcg agctgatgga
     1981 gcgctaccag gtcaccaaca ccttcctgtt cccgaccgcg ctcaagatga tgatgaagag
     2041 cgtgcccgat ccgcgcgagc gctacaagct ggcgctgcgc gccgtcatga gcgcgggcga
     2101 aagcgtgggc gagaccgtgc acgcctggtg cgaatccgcc ctgggcttga cgcccaacga
     2161 aatgttcggc cagaccgaga tgaactacgt ggtgggcaac agcagcaagc gttggccctc
     2221 gcggcccggc agcatgggcc gcccctatcc cggccacaac gtcgccgtga tcgacgaaca
     2281 aggccaggtc tgccccgcgg gcgagatcgg cgaagtggcg gtcaatcgct acgacatcca
     2341 cggccatccc gacccggtga tgttcctggg ctactggcac aacgagcagg ccacgcgcga
     2401 caagttcagc ggcgactggt gccgcacggg cgacctggcc aacgccgatg ccgacggtta
     2461 cctgtggtat gccgggcgcg ccgacgacgt cttcaaatcg gccggctacc gcatcggccc
     2521 gggcgagatc gagaactgcc tgctgggcca tcccgccgtg ggcaacgcgg ccgtggtgcc
     2581 caagcccgac gccgagcgcg gcgcgctggt caaagcctat gtggtgctga cgcccgccta
     2641 ccaggaccgt ccgcgcgacg agatcaccca ggccttgcag gaacacgtcc gcgaccgcct
     2701 ggcgccgtac gaatacccca aggaaatcga atacgtagaa gagctgccca tgaccaccac
     2761 gggcaagatc cagcgcaatg tgctgcgcaa gcgcgagctg gagctggcct cggggcgata
     2821 atctggcaga agcctcaaac gccccggagg gatccgaagc acaggcgcta ccagcgccga
     2881 gcatcggaat cccgcagggg cgtctgaggc cgctcaagaa ctcaaaccga ccgagacaag
     2941 cccatcatgg ccatctacca actggacgac ttcgtccccg acatccaccc cagcgcctac
     3001 gttgccgaca gcgccgacat catcggccag gtcacgctgc gcgaaggcgc cagcatctgg
     3061 tcgcaggtat cgatacgcgg cgacaacgag cccatcacca tcggccgcca cagcaatgtc
     3121 caggaatcca gcgtgctgca cgtggatgcc ggctgcccgc tggtcgtcgg cgagcacgtc
     3181 accgtcggcc accaggccat gctgcatggc tgcaccatcg aagacggcgc cctgatcggc
     3241 atgcaggcca tcgtgctcaa ccgggccgtc gtcggccgca actgcctggt gggcgcgggc
     3301 gccatcatcc ccgaaggccg cgtgattccg gacaactcgc tggtaatcgg catcggcaag
     3361 gtggtgcgcg agctctccgc cgaagaaatc gccaccatgc acgccaacac gcggcattac
     3421 gtcgagcgcg gccagcagta caagaccgcg ctcaagcgca tcgggtagac gggcgggaag
     3481 ccccccacgc tgcgcactac gtgcttgctg cccccgaggg ggcccttttg ccttgaggcg
     3541 gctcggcggc aaaagctcag gccgtcttgg cgatcagggc ctgcatgagc tgctcgcggg
     3601 cctggcggta ttcggggctg ccggggccct ggttgacggc cgtcaacacg aacacccgct
     3661 ggatgcccgc gtcgatggcg ccgaaaggca gcaccgaggt attgatgtcg gcaatgccgc
     3721 ccgtggtggt ggcttcgtcg cgcagcaggt agaggcactc ttcccagatg agcaggcggg
     3781 ccatctgcgc caggacgctt tgcgtatggg ctggcgcggg atacaccagg tgctcggaca
     3841 gccaggcggt gatctggtcg gccggcatgg ggcgggtcag cacatagccc tggatggcat
     3901 cggcccccag gatggccacg gcctcgagga ggtcggcgtt ctcgaccccc tccacgacca
     3961 ccgacttgcc cagggaatgg cccaggcgcg tgagctggta ggtgaagtgc agcacgtcgg
     4021 cggagttctc gccgttgagc atcaccaggc tgcggtcgat cttgaccgtg tcgaaaggca
     4081 gctcgcgcag gcgcatcagg ctgctgtgcc ccgagcccag gtcgtcttcg gccaggccga
     4141 cgcccagttc cttgtagcgc cctagttcgg tggcgacatc gacgcccacc ggcatctcgt
     4201 tggtttcgag gatttcgagc gtgagcaggg aagccgggca gccatgcgcc agcaaggcct
     4261 gcacggtggt gttgtagtag cggacgtcgc ccagcgcgtc gacgggcatg ttgacggaaa
     4321 tgcccagatc catcccgctg cgctgccata cggcccgctg gcgcagggcc tgctccaggc
     4381 cctgggcgta cagcacgaag aagtcttccg aggtcagggc cgggaagaac tcgttgggcg
     4441 acagcagccg gtcgccgtcg cgcaggcgag ccagcgcctc gaccttggtg acacggcgcg
     4501 tcttgaggtc gagcagcggt tggtagtgca tgcacagcgc gtcggacttg agcaaggcgg
     4561 cccagcggcg gcgcacgccg tacgacaccg tctgggtgcc gccttcctgg gcctccaggc
     4621 ggatgatggc gtaggtcagc agcgactgca gctgctcgag aaaggcggtc tggtcgtccg
     4681 agctgaagcc gccggtatgg tcgctgtaca gccccagcac gaccgcgggg ccgatgtccg
     4741 gctggccgac cagcggcacc gccacgatgg agcggaagcg atgcgccagc gccacatccc
     4801 gccagggcgg catgtccgga tcggtggcga tattgatgca atgctcgatg cgattgctgc
     4861 gccaggcctt gtccgcgggg ctgcggccgg accatggcga accggcattg aaatagcccg
     4921 tgcggatctc ctggacgcgc gtcaggtagg actccagccg gggcccttcc agcacctcga
     4981 aattgaaggt ctcctcgctg gagggccggc cgaagaaaca ggcgcagatg ccgggatgcg
     5041 cggccagcag cacggcggtc ttggcgatga gatcggtgta attgatcgcc tcccaggaca
     5101 ggcgcgtgac gttcagcagc aactggacgc gctgggcgta gatttcctgg aagaccgtca
     5161 agtgccaggc caggtcgcgc atcatgcggg cggacagcac ggacagggcc tggctgtgct
     5221 tgtcctgatc gacgtggctg cagaccgcgg cgtagagcag gtccttgctg aaggacagct
     5281 cctccttggt gacgcacgac agcacgtgca cgcgcccgat ggcgcgggcc atggtttcgt
     5341 ggcctttggc agtgaggtcc ggcgccagca gcttgagcag gttggcggcc tcccagcgct
     5401 tgagtttgtc caggtcgtcc gccgacagcg ccttcaatag attttgcgac ttgggcagca
     5461 cccgcaggct gcggtgaaag ccgtcgatga cctgatccac cagggggacc aggtcttgca
     5521 aaaaggtgga caggcccggc atgcaggcct ggccatataa ctgcgattcg tcgtatgccg
     5581 tagcaatagc tattgcagca gcgctatctt gcaaggcagg aatttcgggc atgcacagtc
     5641 ctggctggca cttcacgtta tttgactgga cgcctggcgg caggcgtcct acagatcgga
     5701 atttgaatca tatatgacat tggcgtcaac acatgtaatc ggacactaaa accccatgcc
     5761 cggcaggcca atttaccgga tgggactgca tgtccatgag caggtcgagc cgcaggggca
     5821 aggcgccggc ggccaacgcc tgccgcgtgc aggcatcggt ggcgcccgag gccaatgacc
     5881 gcagcagcgg ccgcaaggcc ggcaccaatg gatccaccag gtagagcaga cgcaggtgca
     5941 gcatattggc ctcgaagatc gtcaaccccg agcgcggccc gcggccctgc ggccatcgcg
     6001 ccgcatagcg cgcatgctgc tccgcttgat aatcattacg aatggcaggc tgcccgcgcg
     6061 agtcgtccgg caagggcaag ccagtctgcc cgaaatcggc atgggccgcc gcatccggct
     6121 gcagcaccca gatacgccag gcgggcagtc cggtacgcgc cgcggtgcgg gcccaggcgg
     6181 cgcgcagccg ttgccgcgag ccgccatccg gcgcggcata gcgcggcgcc atgccggcct
     6241 cgaaggcctg cgccatcaag ctgggacggc cgtgcccggt ggcgcccgcc cgggccgctt
     6301 cggtcaaggc cacctgagcc agatggcgca tgagttgcca gtgcgcgaac tcgagcgcca
     6361 gcaggccggc aaacaggacg acgcccagcg ccacggccga ctcgaccagg gcgctgccgt
     6421 gttggaaggg aaagagaacg cggcgcatgg tcggcagtgt gcttgccgcg aagcgccgcg
     6481 tcgattgtgg gaactacgtt gtggcaatta cgattgtgga aactacgcca ggacaagatc
     6541 gccacgcggt ctcgccgggc tcagtggact gtcggcggat cctgtcgatc cgcgacctgc
     6601 gtgccgcccg cgaacaagcc tgcgcaatcg accgcgtcga acaggtaggg cttgccgcag
     6661 aaatcgcagg agacctccac cttgccttgc tcggccagga tggaccggat ttcggactgg
     6721 cccagcatgc gcagcatgtc ggccacgcgc tcgcgcccgc atgggcaatg ccagcgcacg
     6781 gtttccggct cgaaggcgac cagcgtttct tcccagtaca ggcggcgtat cagcgtatcg
     6841 acgtcagcgg ccagtaactc ggcttcgccc agggtggcgg ccaattgcac cgcgcggttc
     6901 caggtgtcgg aacccgacgc ggcctcgccc ggcgtgccgc cctggtcggg cagccgctgc
     6961 accagcaggc ccacggcatg gtggtcgtcg gcggccagcc acatgcgggt gtcgagctgc
     7021 tcggaggact tcatgtagtg ctccagcacc tcggccacgg tttcgccttc cagcggcacg
     7081 atgccctggt agggctgctg gcccggaacc ttgcgcctgg gatcgagcag cacgatgaag
     7141 cgcccgtcgc catgcgtgtt cagcagcgac tgcagcgtgc ctgtctcggg cacggtgtgg
     7201 ccttcgcgca ccttgaccgt ggcgcgcagg ctgagatcgg cgcggcactc gaccaccagc
     7261 aaagcgatcg gcccgtcgcc ctggatctgc agcagcagcg aaccgtcgaa cttgatgttg
     7321 gacgccagca gggtcgacgc cgccaccagt tcgcccagca gcttgcatac ggcaggcgga
     7381 tattggtggt tggcccgtgc ctcgcgccag gtgtcggtga ggcgcacggc ctgcacgcgg
     7441 gcggtacggt cttcgaatag gtattttttg agctggtctg tcataaagca aatcgttcgg
     7501 cggcgtcagc ggcggggctg cggtcgcggc ggcgcgggct tggcggctgg caccgggggg
     7561 gtattgggaa cggctgcccc cggcggcagg atgttcacga acacctcgcc ttcgcgcatc
     7621 atgccgagct cgccgcgggc gcgctcctcg acggcttcgg tgccgctctc caggttcttg
     7681 acctcggcct gcagggccgc gttgcgggcc cgcaggctgt cgttggtctc gtgctgcgtg
     7741 gcaacctgct tctgcatgtc ccataccttg agccacccgc ccttgcccag ccagagcggg
     7801 tattggatca ggccggccag caagagcagc accaggaaca gcaagcgcat atcagtgccc
     7861 tccgctcaca cttctagcgg atgttgtaga acgcctcgat gccggggtag gaagccacct
     7921 cggcgagttc ttcttcgata cgcagcagct ggttgtactt ggccatgcga tcggaacgcg
     7981 acagcgaccc ggtcttgatc tgcatggcgt tggtggccac ggcgatatcg gcgatggtgg
     8041 agtcctcggt ttcgcccgaa cggtgcgaca cgacggcggt gtagccggcg cgcttggcca
     8101 tttcgatggc ggcgaaggtt tcggtgagcg tgccgatctg gttgatcttg atgaggatcg
     8161 agttggccac gcccttccgg atgccttcgc gcaggatctt ggtgttggtg acgaacaggt
     8221 cgtcgcccac cagctgcacc ttcttgccga gctgatcggt caggagcttc cagccgtccc
     8281 agtcgttttc ggccatgccg tcctcgatgg agatgatggg gtacttgtcg caccaggcgg
     8341 ccagcaggtt ggtgaactct tgcgaagaca gtgacacgcc gccctcgcct tcgagcacgt
     8401 acttgccgtt cttgtagaac tcggaactgg cgcagtccag gcccagggcg atctgggtgc
     8461 cgggctcgta gccggcttcg ccgatcgcct tgaggatgag ctggatggcc gcttcgtggt
     8521 tggccacgtt gggcgcgaag ccgccttcgt cgcccacggc ggtggacatg ccttgcgcgt
     8581 tgatcagctt cttgaggctg tggaagacct cggaacccca gcgcagcgct tcgcgaaagc
     8641 tcgccgcgcc caccggcagg atcatgaatt cctgcatgtc gagggtgttg ttggcgtgcg
     8701 cgccgccgtt gatgacgttc atcatcggca ccggcatgga gagcgggccg ctgccgccaa
     8761 agtagcggta cagcgacagg ccggactcat cggcggcggc gcgggccacg gccatgctgg
     8821 cggccaggat ggcgttggcg cccaggcgtt ccttggactc ggtgccgtcg agttcgatca
     8881 gggtgcgatc gacgaaggtc tgctcctgcg cgtccaggcc catgagggcc tcggagatct
     8941 cggtgttgag gttctcgacg gcgcgcagca cgcccttgcc cagatagcgg tccttgtcgc
     9001 cgtcgcgcag ctcgatggct tcgcgcgcgc cggtggacgc gcccgacgga accgaggcgc
     9061 ggcccatggc gccggattcc agcagcacgt cgcattcgac ggtgggattg ccgcgcgaat
     9121 cgaggatttc gcggccgatg atgtcgacga ttgcactcat gagttcactt ccctgaaacg
     9181 atgaaaatca gtctgatgcc tagcctgcgc aggcccagtc ggcccggtct tcgatgaagc
     9241 cgttgcgctt ggtgatgcgg tcgaggtcgg ccagcgtctc cagcagcgcg gccatctggc
     9301 ccagcggcac ggcgttgggg ccgtcggaca tggcatgcgc cgggtcgggg tgggtttcca
     9361 tgaagaggcc cgccacgccc acggccacgg ccgcgcgcgc cagcaccggc acgaactcgc
     9421 gctggccgcc cgacgacgtg ccctgcccgc cgggcagctg caccgagtgg gtggcgtcga
     9481 acaccaccgg acagccggtt tcgcgcatga tggccagcga gcgcatgtcg gacaccaggt
     9541 tgttatagcc gaacgaagcg ccgcgctcgc acaccatgat ggtgccgccg tcgccgccgg
     9601 cttcgatggc cgcgtcgcgc gccttgttga ccacctgcac catgtcgtgc ggcgccagga
     9661 actggccctt cttgatgttg accggcttga gcgtcgccgc gcaggcgcgg atgaagtcgg
     9721 tctggcggca caggaaggcc ggcgtctgca gcacgtcgac cacagaagcc acgggctcga
     9781 tctgcggcgt gtcgtgcacg tcggtgagca ccggcacgcc gagctccttc ttcacgtcgg
     9841 ccaggatctt caggccttcg tccatgcccg ggccgcggta cgacttgccc gagctgcggt
     9901 tggccttgtc gaacgagctc ttgtagatga agggaatgcc cagcttgccg gcgatttcct
     9961 tgagctggcc ggcggtatcg aaagccatct ggcgcgactc gatcacgcag gggccggcga
    10021 tgaggaagaa gggcttgtcg aggccgacgt cgaagccgca gagcttgttc atggccggct
    10081 ccggcggacc gcccccgggg gggcggcgaa cgaagtgagc gtgggggtcg tcatatcaat
    10141 ctcccttgac gcgccgggcc ttgtgctcga gcgctgcgcg gatgaagctg gagaacagcg
    10201 gatggccgtc gcgcggggtg gaggtgaact ccgggtggaa ctgcacgccc acgaaccagg
    10261 ggtgcgaagg cagctccatc atctcgggca ggtgctcg
//
LOCUS       DASWPO010000196         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2990191, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000196 DASWPO010000000
VERSION     DASWPO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2990191"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGC69_10295"
     CDS             <1..278
                     /locus_tag="VGC69_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0295709.1"
                     /translation="CGAHGAILPTPAPSVMLDGIENGFLVFQVIAYVQSPRLAGGVRS
                     DLLFSMLDGLKDAGLPLAAYAPVLPPGSPMPGERAVAPSTPPTPSAG"
     gene            363..1310
                     /locus_tag="VGC69_10300"
     CDS             363..1310
                     /locus_tag="VGC69_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY0295710.1"
                     /translation="MNIHKNASMTPRGRAHLMREIDRIGLRPAAAAAGLSERTARKWQ
                     RRWVSEGAPGLLDRSSRPERCPRRSDASKVERAVALRRNQRLTYECIAERVGLSRSAV
                     ARACKAAAVARLPALQDTLPVRRYERPSPGELLHLDTKKLGRFDKPGHRVTGDRTQNT
                     PRAGWQALHVAIDDHSRVGFSLMLADETARSACAFLLAALRYYRGLGVKIEQVMTDNG
                     AAYKSRRFAKLLRRLGIRHIRTRPYTPRTNGKAERFIQTLLREWAYAFIYPSSDHRAR
                     ELQPWMYHYNFHRPHSATSHLPPITRLGFDGYNVLRNYS"
     gene            complement(1315..>1614)
                     /locus_tag="VGC69_10305"
     CDS             complement(1315..>1614)
                     /locus_tag="VGC69_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY0295711.1"
                     /translation="GAYGMRQSAFDLFLLYAIGVLGVVMRRFDFPTAPVVVGMILGPL
                     AEAQLRNAMSIGEGSAAVFFQRPMSLVLILVVLAVLILPRVAKAMALRKARARFA"
BASE COUNT          291 a          581 c          499 g          243 t
ORIGIN      
        1 cctgcggggc gcacggcgcc atcctgccga cgcccgcacc ctcggtgatg ctcgacggca
       61 tcgagaacgg cttcctggtc ttccaggtga tcgcctacgt gcagagtccc cggctggccg
      121 gcggcgtgcg cagcgacctg ctgttcagca tgctcgacgg cctgaaggat gccggactgc
      181 cgctggcggc ctacgcaccg gtcctgccgc cgggatcacc gatgcccggc gagcgggcgg
      241 tcgcgccgtc cacgccaccc acaccatcgg ccggctagct gtagtttccc aagaggttgt
      301 tcatccgagg tgaaggaaga tggaggttct caagccaaac cacttccttc aggactcccc
      361 ggatgaacat ccacaagaat gcctcaatga cgccgcgagg tcgagcgcat ctgatgcgcg
      421 agatcgatcg gatcgggctc aggcctgcgg ctgcagccgc aggcctgagc gaacgcaccg
      481 cccgcaagtg gcagcgccgt tgggtcagcg agggcgcacc tggcctgctc gaccgcagtt
      541 cccgccccga acgctgtccc cggcgcagcg atgcctccaa ggttgaacgc gccgtggcgc
      601 tgcgccgcaa tcagcgcctg acctatgaat gcattgccga gcgtgtgggc ctgtccagaa
      661 gcgccgtggc gcgagcctgc aaggccgctg ccgtggcccg gttgccagcg ttgcaggaca
      721 cgctgcccgt gcggcgctat gagcgcccgt cgcccggcga actgctccac ctggatacca
      781 agaagctcgg gcgcttcgac aagcctggcc accgtgtcac gggcgaccgc acgcagaaca
      841 ccccgcgcgc gggctggcag gcattgcacg tggccatcga cgatcactcg cgcgtgggat
      901 tcagcttgat gctcgcggac gaaacggcca gaagcgcttg cgccttcctg ctggcggcgc
      961 tgcgctatta cagaggcctg ggtgtgaaga tcgagcaggt gatgacggac aacggcgctg
     1021 cctacaagtc acgccgcttc gccaagctct tgcgccgcct gggcatccgg cacattcgca
     1081 ctcgcccgta cacgccgcga accaacggca aggccgagcg gttcatccag acgctgctgc
     1141 gcgaatgggc ctacgccttc atctacccca gctcagacca tcgagcccgc gaactgcaac
     1201 cctggatgta ccactacaac ttccatcgac cacactccgc cacctctcat ctccctccca
     1261 tcactcgcct tggcttcgac gggtacaacg tgttgagaaa ctacagctag ccgatcaagc
     1321 gaaccgggcg cgcgccttcc tgagcgccat cgccttcgcc acccgcggca ggatcagcac
     1381 cgccagcacg acgaggatca gcacgagcga catgggccgc tggaagaaca ccgccgcgct
     1441 gccctcgccg atggacatcg cgttgcgcag ttgcgcctcg gccagcggac cgaggatcat
     1501 gcccaccacc accggcgctg tcgggaagtc gaagcgccgc atgaccacgc ccagcacgcc
     1561 gatggcgtag agcaggaaca ggtcgaacgc gctctggcgc atgccgtagg cgcc
//
LOCUS       DASWPO010000197        11362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_131937, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000197 DASWPO010000000
VERSION     DASWPO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131937"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VGC69_10310"
     CDS             complement(<1..1024)
                     /locus_tag="VGC69_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0295712.1"
                     /translation="MPSPEYDIAILGAGPVGQALALLLSRLAPDPARIALLAGDLGTA
                     QPAPADDPRIIAINHGSRVMLESLQAWPARSAAIGHIHVSQRGRLGRTVIAGEDFGVP
                     ALGNTIAYADLHARLLERVAASGITLLSGPPATIAAQDAGMVGIRQGDTTLACSIAVR
                     CDGDGGQDVKRDYGQHAVLTRASATLARPGWAWERFTREGPLALLPHPQMNGDYAVVW
                     CCAPDHAARIAQAGDADFSAALTEAFGTRLGRLHSSAPRQVFPLHLKARHAQAQGRVA
                     TIGNAAQTLHPVAGQGLNLGLRDAAQLAQALGQWLAARQTGRNEAEAQADDPAPLLAD
                     FARTRLP"
     gene            complement(1102..2448)
                     /locus_tag="VGC69_10315"
     CDS             complement(1102..2448)
                     /locus_tag="VGC69_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY0295713.1"
                     /translation="MSLPSTDIAPFRERRRLLLAHLRAQGGGIAVLPTAPQAVRNRDG
                     EYPYRHDSDFYYLSGFVEPEAWLVLIAGADDHALLFCRPRNPEAETWEGYRVGPEAAA
                     ERFGFDAAYDVGELDEILPELLLDQPALYAPFDRGDVVDGPLRGWLEAARGQARSGRR
                     APQRMQNLSSIVAEMRLVKDAAEIATMRRAARISAGAHARAMRTVRPGMHEYELEAEL
                     LYEFRRHGAQAPAYNSIVAGGANACILHYPAGDAELRDGDLVLIDAACELDSYASDIT
                     RTFPVNGRYSGPQRALYDLTVAAQQAAADATRPGQPWNAGHQAALRVLAQGMLDEKLL
                     KGTLDGVLESGDYRRFYMHQTGHWLGLDVHDVGDYRDPAAALDDGSRPWRQLQPGMML
                     TIEPGIYVTPAEDVPAKFWNIGIRTEDDALVTETGCELITRGVPVDAKEIEALMRE"
     regulatory      2493..2605
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            2692..3696
                     /locus_tag="VGC69_10320"
     CDS             2692..3696
                     /locus_tag="VGC69_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEY0295714.1"
                     /translation="MLFTLFLFLLSAVIIYLACEYFVNGVEWVGHRFSLGATATGTVL
                     AAFGTALPESVVTFMAVAFGQTPEQKDIGVGAAMGGPLVLSTVAYAVVGLVLLAVARK
                     GGHAPAIGQDDQQRLARDQAWFMAVFVFKVGLGLLAFAWKPWLGILFLAVYAVYVKRE
                     LASDEAGVDHEDLEPLKLRPGDDRPTMFWAGAQTLLSLVVIAVASRVFVHQIGTLGEA
                     MGASPHVAALLLAPVATELPEILNALIWVRQGKARLALANISGAMMIQATVPSALGIF
                     MTPWLFDAPLLVSGLVTLAAIVLLWLRLRQGRMTARLLVWSGSLYVAFAAWLVWHFST
                     "
     gene            4065..4379
                     /locus_tag="VGC69_10325"
     CDS             4065..4379
                     /locus_tag="VGC69_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295715.1"
                     /translation="MFGLFLRRATRTGFAARQGASRLTVECPREALQALRRQICLEFA
                     SAGLNVSRLQVGSGSQPGLASACVTVVCPPERRAELMSQAQRLSGHPAVRLVRLGAMP
                     AY"
     gene            4626..7370
                     /gene="mgtA"
                     /locus_tag="VGC69_10330"
     CDS             4626..7370
                     /gene="mgtA"
                     /locus_tag="VGC69_10330"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEY0295716.1"
                     /translation="MLQFFKVLFSPAAQVRRAGRHFRRGPMLEQVGHDAGRAAVVEAA
                     RQAGGDIVALARAPLADVYARFGSGPQGLDAGHAEQIRTAFGPNEVEHERPLSLATHL
                     WLSFRNPFNLLLAALAVLSCVTDVIMATPGEQSWASVIIIGAMVAIATLLRLVQERRS
                     NKAADALRAMVQNTATVLRPGQGRGVEMPLRELVPGDVVTLSAGDMIPADCRIVAAKD
                     LFVAQAALTGESLPVEKFAQAGHTAGNPLELENIGFMGTNVVSGAGLALVIGTGSRTC
                     FGQLASSVTQAANTPTQFQQGINRVSWLLIRFMLVMVPVVFVLNGLTKHDWLEALLFA
                     LAVAVGLTPEMLPMIVTSTLAKGAVAMARKKVVVKRLDAIQNLGAMTVLCTDKTGTLT
                     QDRIVLERHVNIDGVASDEVLAYAYLNSHYQTGLKNLLDVAVLEHAEVGRRLNPAANY
                     DKIDEVPFDFSRRRMSVVVSERGREASGHILICKGALEEMLAACTRVRGDDGDIDLDE
                     NGRADIRRTAAGMNAEGLRVIAVGIKRLPAGHGVYSVADEAGLTLVGYIAFLDPPKES
                     TAPALRALLASGVEVKVLTGDVERVARKVCKEVGLDVRGEYTGPELEAMDDAALAEAA
                     RQGTLFARLAPAMKERIVRSLRAQGATVGFMGDGINDAPALRAADVGISVDSAVDISK
                     EAADIILLEKSLTVLEDGIVEGRRIFNNMLKYLKMTASSNFGNVFSVLIASAFLPFLP
                     MLPIQLLAQNLMYDFSQIAIPFDRVDAEQLRQPQQWDPAGLGRFMVFFGPISSIFDVA
                     TFAMMWFVFHADHVGAQSLFQSGWFVEGLLSQTLVVHLIRTARIPFLQSRAAASLLVM
                     TGAVMLAGLLLPFSPLAEYFHMQPLPWLYFAGLIVILLAYAALVQWMKGLWIKRHGWS
                     "
     gene            7380..8528
                     /locus_tag="VGC69_10335"
     CDS             7380..8528
                     /locus_tag="VGC69_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0295717.1"
                     /translation="MKAGRKIQKTFNKLGPISRITNGTLYAGGAAMVLSLCAGGMARA
                     DDGVTVYGLIDDSIASTHTGGTRTRPGNNQVSLLTGGWTDSLWGIKGEESLDGGWKAR
                     FGLESGIDSATGRSLDGQDRLFDYGAWVGLDHAGYGAVKLGRLSTVAQEYGGQLEVAS
                     WRDMGMGQLLKDSDNFRRDRTLNLYTPDIGGAQLGVGRSFNDRSGVPDMQAWSLGFKY
                     ASGPWLGVLTWDRSSRALGDSHGGSPSAAQAGVTYITDDGYQFSVAWSRQRNGFVEQD
                     IGWSGGPAAFMYGGYADSWLVGAQIPVSTSGSVLMQVSQDRPSWHEADGSRAHPVALA
                     TLGYRYTLSPRTSLYAFIGRQTRGSLADPFDHDTGGVTRVAVGVTHSF"
     gene            complement(8542..9381)
                     /gene="galU"
                     /locus_tag="VGC69_10340"
     CDS             complement(8542..9381)
                     /gene="galU"
                     /locus_tag="VGC69_10340"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY0295718.1"
                     /translation="MQPIRKAVFPVAGFGTRFLPATKAMPKEMLPVVDKPLIQYAVEE
                     AVAAGITDLIFVTGRNKRAIEDHFDAAPELERDLEEKGKNELLAVVRGVIPPQVTCMF
                     IRQKAMLGLGHAVLTAAPAVGNEPFAVLLADDLIDADNSVTKQLMDTAMTHGVSVLGV
                     QEVPHADTGKYGIVASEMLNARTARVTHIVEKPKPEDAPSNLAVVGRYVLEPAIFDHL
                     RKTERGAGDEIQLTDAIAALLREREVYAHRYEGTRYDCGSKAGMFQATVAIGRKYHGL
                     IPE"
     gene            9525..11030
                     /locus_tag="VGC69_10345"
     CDS             9525..11030
                     /locus_tag="VGC69_10345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0295719.1"
                     /translation="MLPNARRALAGALCLALSLPGLPAAAQPVGLPSMGAASGYDLSP
                     AQERQLGEAIMVQGRRDPAYIDDAELNQYLTAMGRKLAAFAPNPVPLVELFGVLDPEI
                     NAFTMPGGFIGVNSGLVLTCESESELAAVLGHELGHVAQRHIARGMTQGGQSGAVALA
                     SLAAALLAALAGSGDLAMGIVAFGQAAALSQQLGFSRDAEREADRAGLQMLYKAGYDP
                     SAMARIFGRLMNASRLNEGMGGGAWASTHPQSIERMSDMQNRIQEMPVAHYADSADFW
                     LLRAKLRVLQGGDSQALLYVREQLQAESASSQGVQRAAALYGLSRYWLRREDLKQAAS
                     YWEQAAAAWPAGAPELARLGIDITLARKDAPGAVGQAQAAIKRWPGQRALGMAYVTAL
                     QAAGRQAEAQNYLRDRVKAWGSDQPDLYQMLAKSEDASGNPVAARSDMAQYYLLTGAY
                     AAAAMQLQQARRISTDFYEQSQLDAQLRDTRQRLQDERNLLEKFKDVVPAG"
     gene            complement(11058..>11362)
                     /locus_tag="VGC69_10350"
     CDS             complement(11058..>11362)
                     /locus_tag="VGC69_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0295720.1"
                     /translation="HDFDDAYTAPMHGFRNALDYWTRASSKPWLASVRVPTLVLNARN
                     DPFLPSQALPAPEDCSARVLLHQPEQGGHAGFPTGRFPCSLNWLPGRLGRFFETGT"
BASE COUNT         1632 a         3716 c         4117 g         1897 t
ORIGIN      
        1 cgggcaggcg ggtgcgggcg aaatcggcga gcaggggcgc cgggtcgtcg gcctgggctt
       61 ccgcctcgtt gcggccggtt tgccgtgctg ccagccactg gcccaaggcc tgggccagtt
      121 gggcggcatc gcgcaggccg aggttcaggc cctggcccgc gacgggatgc agggtttgcg
      181 cggcattgcc gatggtggcg acgcgtccct gggcctgggc gtggcgggcc ttgagatgca
      241 gggggaagac ctggcgcggg gcgctgctgt gcaggcggcc caggcgggtg ccgaaggcct
      301 cggtgagcgc ggcggagaag tcggcgtcgc cggcctgggc gatccgggcg gcgtggtcgg
      361 gcgcgcagca ccagacgacg gcgtagtcgc cgttcatctg cggatggggc agcagggcca
      421 gcgggccttc ccgggtaaag cgttcccagg cccagcccgg gcgcgccagg gtggcgctgg
      481 cgcgggtgag cacggcgtgc tggccgtagt cgcgcttcac gtcctggccg ccgtcgccgt
      541 cgcagcgcac ggcgatggaa caggccagcg tggtgtcgcc ttgcctgatg ccaaccatgc
      601 ccgcgtcttg cgccgcgatc gtggcgggcg ggccggacag cagggtgatg ccgctggcgg
      661 cgacgcgctc gagcaggcgg gcgtgcaggt cggcataggc gatggtgttg cccagggccg
      721 gcacgccgaa gtcttcgccg gcgatgacgg tgcggcccag gcggccgcgc tgcgagacgt
      781 ggatgtggcc gatggcggcc gagcgcgcgg gccaggcttg caggctctcc agcatcacgc
      841 ggctgccgtg gttgatggcg atgatgcgcg ggtcgtcggc cggcgcgggc tgcgccgtgc
      901 cgagatcgcc agccagcagg gcgatgcggg cgggatcggg agccaggcgc gacaacagca
      961 gggccagcgc ctggcccacg ggaccggcgc cgaggatggc gatgtcgtat tcaggggaag
     1021 gcatgcctgg attttaccgg tcgaggcatg cggctgaaga acgcctatgc ggcgattcag
     1081 gaatgcaaat ccttgcccgc ctcattcccg catcaaggct tcgatttcct tcgcatccac
     1141 cggcaccccc cgcgtgatga gttcgcatcc cgtttcggtg accagcgcgt cgtcctcggt
     1201 gcgtatcccg atgttccaga acttggcggg cacgtcctcg gccggggtga cgtagatgcc
     1261 gggctcgatg gtgagcatca tgccgggctg gagctgccgc caggggcgcg agccgtcgtc
     1321 gagcgcggcc gccgggtcgc ggtagtcgcc gacatcgtgc acgtccagcc ccagccagtg
     1381 gccggtctgg tgcatgtaga agcggcggta gtcgcccgac tcgagcacgc cgtcgagcgt
     1441 gcccttgagc agcttctcgt cgagcatgcc ctgggccagc acgcgcaggg cggcctggtg
     1501 gccggcgttc cagggctggc cggggcgggt ggcgtcggcg gccgcctgct gggccgcgac
     1561 ggtcaggtcg tagagcgcgc gctgcgggcc gctgtagcgg ccgttgacgg gaaaggtgcg
     1621 ggtgatgtcc gaggcgtagc tgtcgagctc gcaggcggcg tcgatcagca ccaggtcgcc
     1681 gtcgcgcagt tcggcgtcgc ccgcggggta gtgcaggatg caggcgttgg cgccgccggc
     1741 gacgatgctg ttgtaggccg gggcctgggc gccgtggcgg cggaattcgt agagcagttc
     1801 ggcttcgagc tcgtattcgt gcatgccggg acgcacggtg cgcatggcgc gggcgtgcgc
     1861 gccggccgag atgcgcgcgg cgcggcgcat ggtggcgatc tcggcggcat ccttgaccag
     1921 gcgcatctcg gccacgatgg acgagaggtt ctgcatgcgc tgcggcgcgc ggcggcccga
     1981 acgggcctgg ccgcgagcgg cctcgagcca gccgcgcagc gggccgtcga cgacgtcgcc
     2041 gcggtcgaag ggggcgtaga gcgcaggctg gtcgagcagc agttcgggca ggatctcgtc
     2101 gagctccccg acgtcatagg ccgcatcgaa accgaagcgc tcggcagcgg cctcggggcc
     2161 gacgcgatag ccttcccagg tttccgcctc ggggttgcgc ggtcggcaga acagcagggc
     2221 gtggtcgtcg gcgccggcga tcagcaccag ccaggcctcg ggttcgacga agccgctcag
     2281 gtagtagaag tcgctgtcgt ggcgataagg gtattcgccg tcgcggttgc gcacggcctg
     2341 cggcgcggtg ggcagcacgg cgatgccgcc gccctgggcg cgcaggtgcg ccagcagcag
     2401 ccggcggcgt tcacggaaag gggcgatgtc ggtggagggc aggctcatgc gggtacttta
     2461 ctcgcggggg ggcgctgcta caatctgccc gcacttccgt cattggggag tagccatccc
     2521 tgcaagggga gcctgcgtca acagacttgg cggtcatgga ccccatggcg caggcagcct
     2581 gcggcgcatt tcgcgccagc ggcccggcga gacctttggc cacgtcgcgt tcaccctgcc
     2641 gggcgagccg cggcgttgcg ccattgtcgc tgcttgcccg gccatcgtcc catgctgttc
     2701 accctgtttc tgttcctgct atccgcggtc atcatctacc tggcctgcga atacttcgtc
     2761 aacggcgtcg aatgggtggg acatcgcttt tcgctgggcg ccacggccac gggcacggtg
     2821 ctggcggcct tcggcacggc cttgccggaa agcgtggtca ccttcatggc ggtggcgttc
     2881 ggccagacgc ccgagcagaa ggacatcggc gtgggggcgg ccatgggcgg gccgctggtg
     2941 ttgtccacgg tggcgtacgc ggtggtcggc ctggtgttgc tggcggtggc ccgcaagggc
     3001 gggcacgcgc cggccatcgg ccaggacgac cagcagcgcc tggcgcgcga ccaggcctgg
     3061 ttcatggcgg ttttcgtgtt caaggtgggg ctgggcctgc tggcctttgc ctggaagccc
     3121 tggctgggca tcctgttcct ggcggtgtat gccgtctacg tcaagcgcga actggccagc
     3181 gacgaggccg gcgtggacca tgaggatctc gagccgctca agctgcgtcc gggcgacgac
     3241 cgtcccacga tgttctgggc gggcgcgcag acgctgctgt cgctggtggt gatcgccgtg
     3301 gcctcgcgcg tgttcgtgca tcagatcggc acgctgggcg aggccatggg cgcctcgccg
     3361 cacgtggcgg ccctgctgct ggcgccggtg gccaccgagc tgccggaaat cctcaatgcg
     3421 ctgatctggg tgaggcaggg caaggcgcgc ctggccctgg ccaatatctc gggcgccatg
     3481 atgatccagg ccacggtccc cagcgccctg ggcatcttca tgacgccgtg gctgttcgat
     3541 gcgccgctgc tggtttcggg cctggtcacg ctggccgcga tcgtcctgct ctggctgcgc
     3601 ctgcgccagg ggcgcatgac ggcgcggctg ctggtctggt cgggcagcct ctatgtggcg
     3661 ttcgccgcct ggctggtctg gcatttcagc acctgagccg atgtgcggcg tttgtgcatc
     3721 atgcgcgcga tgccgggcaa aaagcccttg tttgcgggga tagcccggga ctacaatttc
     3781 aaggtcttca tttccagggg ttctctaccg tgagtaccga ttccggcgac agtagtagtc
     3841 gatctcgcat cgacgccatt gcctgacagg tatccagcag agcctcttct gccgcatcct
     3901 gcctgccagt gcaagggtgt ggtttcttcc cccagttcta ccaccgccag aaccgccgcg
     3961 gcccgcgcgt gcatgcgtcc tgcctgtctc ttgaccgggc gggtgctgcc gcgagcctgc
     4021 ccgtatccac ggcagacgga ttccgttcgt gcacggaggt cgtcatgttc ggtctgtttc
     4081 tgcgtcgcgc cacccggacg ggttttgccg cccgccaggg tgcgtcgcgc ctgacggtgg
     4141 agtgtccgcg cgaggccctg caggccctgc gccggcagat ctgcctggag tttgcctcgg
     4201 ccggcctgaa cgtgtcgcgc ctgcaggtgg ggtcgggcag ccagcccggc ctggcctcgg
     4261 cctgcgtcac ggtggtctgt cctcccgagc gccgcgccga gctgatgtcc caggcccagc
     4321 gcctgagcgg ccatccggcg gttcgcctgg ttcgcctggg cgccatgccg gcgtattgat
     4381 tccatccatt tttcaaaagg tcacgccatg tcaggttcac tcgatcccga gcctcgattg
     4441 cgccggccgt gcaaggccgg gcgccgcgcc tgacccgctt cctttttccg gcgggccggc
     4501 agcagcccgt acggattctt ccccttgcgc caccggcgcg acgaacgctt cccttccggc
     4561 catgccgtgc atcgcaagat gcatggcatg gacttcgttg aaataaaaaa accgggagca
     4621 gtgccatgct gcagtttttc aaagtcctgt tttcgccggc cgcgcaggtc cggcgcgcgg
     4681 gccgtcattt ccgtcgcggc cccatgctcg agcaggtcgg ccatgacgcc ggccgcgccg
     4741 ccgtcgtcga ggccgcgcgc caggcgggcg gcgacatcgt cgcgttggcg cgggcgccgc
     4801 tggccgacgt gtacgcgcgt ttcggcagcg gcccgcaggg cctggatgcc gggcacgccg
     4861 agcagatccg cacggccttc ggtcccaatg aggtcgagca tgaacgtccg ctgtcgctgg
     4921 cgacgcacct gtggctgtcg tttcgcaatc ccttcaacct gctgctcgcc gcgctggccg
     4981 tcctgtcgtg cgtgaccgac gtgatcatgg ctacgcccgg cgagcagagc tgggcttcgg
     5041 tcatcatcat cggcgccatg gtggcgatcg ccacgctgct gcgcctggtg caggagcggc
     5101 gctcgaacaa ggcggccgac gccttgcgcg ccatggtgca gaacaccgcc accgtgctgc
     5161 gcccggggca gggccgcggc gtggaaatgc cgctgcgcga gctggtgccc ggcgacgtgg
     5221 tcaccctgtc ggccggcgac atgatcccgg ccgactgccg catcgtggcc gccaaggact
     5281 tgttcgtggc gcaggccgcg ctcaccggcg agtcgctgcc ggtggagaag ttcgcgcagg
     5341 caggccacac ggccggcaat ccgctggaac tggagaacat cggcttcatg ggcaccaacg
     5401 tggtcagcgg cgccggcctg gcgctggtga tcggcacggg ctcgcgcacc tgcttcggcc
     5461 aactggccag ctcggtcacg caggcggcga acacgcccac gcagttccag cagggcatca
     5521 accgcgtgag ctggctgctg atccgcttca tgctggtcat ggtgccggtg gtgttcgtgc
     5581 tcaacgggct gaccaagcac gactggctgg aggcgctgct gttcgccctg gccgtcgcgg
     5641 tgggactgac gcccgagatg ctgcccatga tcgtcacctc gaccctggcc aagggggcgg
     5701 tggccatggc gcgcaagaag gtggtggtca agcgcctgga cgccatccag aacctgggcg
     5761 ccatgacggt gctgtgcacc gacaagaccg gcacgctcac gcaggaccgc atcgtgctcg
     5821 agcgccacgt caatatcgac ggcgtggcca gcgacgaggt gctggcctat gcctacctca
     5881 acagccacta ccagacgggc ctgaagaacc tgctcgacgt cgcggtgctg gagcatgccg
     5941 aggtgggacg ccggctcaac cctgcggcca actacgacaa gatcgacgag gtgcctttcg
     6001 atttctcgcg ccgccgcatg tcggtggtgg tgagcgaacg cggccgcgag gcgtccggcc
     6061 atatcctcat ctgcaagggg gcgctggagg aaatgctggc ggcctgcacg cgcgtgcgcg
     6121 gcgacgacgg cgacatcgat ctcgacgaga acggccgggc cgacatccgc cgcacggcgg
     6181 ccggcatgaa cgccgagggc ctgcgggtga tcgccgtggg catcaagcgc ctgccggccg
     6241 gccatggcgt ctacagcgtg gccgacgagg ccgggctgac gctggtgggg tatatcgcct
     6301 ttctcgatcc gcccaaggaa tcgacggcgc cggcgttgcg cgccctgctg gcctcggggg
     6361 tcgaggtcaa ggtgctgacc ggcgacgtcg agcgggtggc gcgcaaggtg tgcaaggagg
     6421 tcgggctgga cgtgcgcggc gaatacaccg gccccgagct cgaggccatg gacgacgccg
     6481 cgctggccga ggccgcgcgg cagggcacgc tgttcgcccg gctggcgccc gcgatgaagg
     6541 agcgcatcgt gcgcagcctg cgcgcgcagg gcgccacggt gggcttcatg ggcgacggca
     6601 tcaacgacgc gccggcgctg cgcgcggccg acgtgggcat ttcggtggac tcggccgtgg
     6661 acatctcgaa agaggcggcc gacatcatcc tgctggaaaa gagcctgacc gtgctcgagg
     6721 acggcatcgt cgagggccgg cgcatcttca acaatatgct caagtacctg aagatgacgg
     6781 ccagctcgaa cttcggcaat gtgttctcgg tgctgatcgc cagcgccttc ctgccgtttt
     6841 tgcccatgct gcccatccag ctgctggcgc agaacctgat gtacgacttc tcgcagatcg
     6901 ccataccctt cgaccgcgtc gacgccgagc agctcaggca gccgcagcaa tgggacccgg
     6961 ccggcctggg gcgcttcatg gtgttcttcg gtccgatcag ctcgatcttc gacgtggcca
     7021 ccttcgccat gatgtggttc gtcttccatg ccgaccacgt gggcgcccag tccctgttcc
     7081 agtcgggctg gttcgtcgag ggcctgttgt cgcagaccct ggtcgtgcac ctgatccgca
     7141 cggccaggat tcccttcctg caaagccggg ccgccgcgtc cctgctggtc atgacgggcg
     7201 cggtcatgct ggcgggcctg ctgctgcctt tttcgccgct ggccgagtat ttccatatgc
     7261 agcccctgcc ctggttgtat ttcgccggac tgatcgtcat attgctggcc tatgctgcgc
     7321 tggtacagtg gatgaaaggt ctctggatca agcgccacgg ctggagctga gacgggaaaa
     7381 tgaaagccgg tcgaaaaata caaaagacat tcaacaagct cggcccgatc agtcgcatca
     7441 cgaacggcac tttgtatgcc ggcggcgcgg cgatggtcct gtccttgtgc gcgggcggca
     7501 tggcccgggc cgacgacggc gtgacggtct atggcctgat cgacgacagc atcgccagca
     7561 cccataccgg cggcacccgg acccggccgg gcaacaacca ggtgagcctg ctcaccggcg
     7621 gctggacgga cagcctctgg ggcatcaagg gcgaggaatc gctcgacggc ggctggaagg
     7681 cgcgcttcgg cctggaaagc ggcatcgatt cggccacggg ccgttcattg gacgggcagg
     7741 accggctgtt cgattacggc gcctgggtcg ggctggacca tgccggctac ggcgcggtga
     7801 aactgggccg cttgagcacc gtcgcccagg aatacggcgg ccagctcgag gtggcctcgt
     7861 ggcgcgacat gggcatgggc cagctgctca aggattccga caatttccgg cgcgaccgga
     7921 cgctcaacct gtacacgccc gatatcggcg gcgcgcagct gggcgtgggc cgcagcttca
     7981 acgaccgcag cggcgttccc gatatgcagg cgtggagcct gggcttcaag tacgccagcg
     8041 gtccctggct gggcgtgctg acctgggacc gcagcagccg tgccctgggc gactcccatg
     8101 gcggatcgcc ctcggccgcg caggccggcg tcacctacat caccgatgac ggctaccagt
     8161 tctcggtggc ctggtcgcgc cagcgcaacg gcttcgtgga gcaggacatc ggctggtccg
     8221 gcgggccggc ggcgttcatg tacggcggct atgcggacag ctggctggtc ggcgcgcaga
     8281 tcccggtcag cacgtcgggc tcggtgctga tgcaggtgtc ccaggaccgg ccgagctggc
     8341 atgaggccga cggcagccgc gcgcatcccg tggcgctggc cacgctgggc taccgctata
     8401 cgctgtcgcc gcgcaccagc ctctatgcct tcatcggccg ccagacgcgc ggttcgctgg
     8461 ccgacccctt cgaccacgac acgggcggcg tgacgcgcgt ggcggtcggg gtgacccact
     8521 cgttctgagc ggggctttgc cttattcggg gatcaggccg tggtacttgc ggccgatggc
     8581 cacggtggcc tggaacatgc cggccttgct gccgcagtcg tagcgggtgc cttcgtagcg
     8641 gtgggcgtag acctcgcgct cgcgcagcag cgcggcgatg gcatcggtga gctggatttc
     8701 gtcgccggcg ccgcgttcgg tcttgcgcag gtggtcgaag atggcgggtt cgagcacgta
     8761 gcggcccacc acggcgaggt tggagggcgc gtcttcgggc ttgggcttct cgacgatgtg
     8821 cgtgacgcgc gcggtgcggg cgttgagcat ctcgctggcg acgatgccgt acttgccggt
     8881 gtccgcgtgg ggcacttcct gcacgcccag cacgctgacg ccgtgggtca tggcggtgtc
     8941 catcagctgc ttggtgacgg agttgtcggc gtcgatcagg tcgtcggcca gcagcacggc
     9001 gaagggctcg ttgcccacgg ccggcgcggc ggtcagcacg gcgtggccca ggcccagcat
     9061 cgccttctgg cggatgaaca tgcaggtgac ttgcggcggg atcacgccgc gcaccaccgc
     9121 cagcagctcg ttcttgccct tttcttcgag gtcgcgctcg agttcgggcg ccgcgtcgaa
     9181 gtggtcttcg atcgcgcgct tgttgcgacc ggtaacgaag atcaggtcgg tgatgccggc
     9241 cgcgacggct tcttccaccg cgtattggat gagcggttta tctaccacgg gcagcatttc
     9301 cttgggcatg gccttggtgg ccggcaggaa acgcgtgcca aaacccgcga cggggaagac
     9361 ggctttgcgg ataggctgca tcgaaaagaa ctccagtgaa aattaaaatg tgaattcgtc
     9421 aggctgccga aagaaaggct gtaaagaggg ccataaaaaa gggccaaaaa actttgcaaa
     9481 caggccgcta tcatagtgcc tattacattt tgggcatgat accgatgttg ccgaatgcaa
     9541 ggcgcgcgct ggccggagcg ctctgtctgg cgctatccct gcccggcctg ccggcggcgg
     9601 ctcagccggt cggcctgcct tccatggggg cggcttccgg atacgacctc tccccggccc
     9661 aggagcgcca gctgggcgag gccatcatgg tgcagggccg gcgcgaccct gcctatatcg
     9721 acgacgccga gctcaaccag tacctcacgg ccatgggccg caagctggcg gccttcgcgc
     9781 ccaacccggt tccgctggtc gagctgttcg gggtgctcga cccggagatc aacgccttca
     9841 ccatgccggg cggcttcatc ggggtgaatt ccggcctggt cctgacctgc gagagcgaat
     9901 ccgaactcgc ggcggtgctg ggccatgagc tcggccacgt cgcccagcgc cacatcgccc
     9961 gcggcatgac ccagggcggc cagagcggcg ccgtggcgct ggcctccctg gcggcggccc
    10021 tgctggccgc gctggcgggc tcgggcgacc tggccatggg catcgtcgcc ttcggccagg
    10081 ccgccgcgct cagccagcag ctgggcttct cgcgcgatgc cgagcgcgag gccgaccgcg
    10141 ccggcctgca gatgctctac aaggccggct acgatcccag cgccatggcc cgcatcttcg
    10201 gccgcctgat gaatgcctcg cgcctgaacg aaggcatggg cggcggcgcc tgggccagca
    10261 cccatccgca gtccatcgag cgcatgtccg acatgcagaa ccgcatccag gagatgcctg
    10321 tcgcgcacta tgccgacagt gccgatttct ggctcctgcg ggccaagctg cgcgtgctgc
    10381 agggcggtga ttcgcaggcg ctgctttatg tgcgcgagca gttgcaggcc gagtcggctt
    10441 ccagccaggg cgtgcagcgt gccgccgcgc tctacggctt gtccaggtac tggctgcggc
    10501 gcgaagacct gaagcaggcc gcctcgtact gggagcaggc cgcggcggcc tggcccgccg
    10561 gggcgcccga gctggccagg ctgggcatcg acatcaccct ggcccgcaag gatgcgccgg
    10621 gcgccgtcgg gcaggcgcag gccgcgatca agcgctggcc cggacagcgg gcgctgggca
    10681 tggcgtatgt cacggccctg caggccgccg gccggcaggc cgaggcgcag aactacctgc
    10741 gcgaccgggt caaggcctgg ggctcggacc agcccgacct gtaccagatg ctggccaaga
    10801 gcgaggatgc cagcggcaac ccggtggcgg cgcgcagcga catggcgcag tactacctgt
    10861 tgacgggcgc ctacgccgcc gcggcgatgc agctgcagca ggcgcggcgg atctccaccg
    10921 atttctatga gcagtcgcag ctcgatgcgc aactgcgcga cacccggcag cgcctgcagg
    10981 acgagcgcaa cctgctcgag aaattcaagg acgttgtccc ggccggctag gcgtggggcc
    11041 cggctggcgc gccaaggcta ggtgcctgtt tcgaagaaac ggcccagccg cccgggcagc
    11101 cagttcaggc tgcaggggaa cctgccggtc ggaaaaccgg catggccgcc ttgttcgggc
    11161 tggtgcagca gcacgcgggc cgaacagtct tcgggcgcgg gcagcgcctg gctgggcagg
    11221 aaggggtcgt tgcgcgcatt gagcaccagg gtgggcacgc gcaccgacgc cagccagggc
    11281 ttgctcgatg cccgcgtcca gtaatcgagc gcattgcgaa agccgtgcat gggcgcggtg
    11341 taggcatcgt cgaaatcgtg ca
//
LOCUS       DASWPO010000198         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1294831, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000198 DASWPO010000000
VERSION     DASWPO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294831"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGC69_10355"
     CDS             <1..330
                     /locus_tag="VGC69_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0295721.1"
                     /translation="LGTIAMFGIIMRNSVILVDQIEQDIRGGAPRWQAIIGATVRRFR
                     PITLTAAAAVLALIPLLRSNFFGPMATALMGGITVATVLTLFYLPALYAAWFRVRADE
                     PAKEAQP"
     gene            327..1796
                     /locus_tag="VGC69_10360"
     CDS             327..1796
                     /locus_tag="VGC69_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0295722.1"
                     /translation="MNAALAMATAAGAARLLKRGLLPATMALALAGCAFEAPTKPPAL
                     PDPARYTAAPLPADTVAADGTAQHFSLGERPVPQWWRRYGSETLDAWIDEGLRNSPTL
                     ESAGHALAAARETLRGQIGAVELPTVDVQVQTERQRALGLPAFGQPTNLYNVYTGQLA
                     LSYDLDLSGAARYGIAQVTAQVDRQSFELDTARRTLAANIVMAAISAASADAQRQAAE
                     RLAALAHSRGDIAARARGLGAASPADVLTVQQEAASLDAALPALRAQTQRARHALAVL
                     MGRSPDQAPPVLPLDDLRLPDDVPVSVPSELLHQRPDVLAAEAGVRISAAQIGVATAN
                     LFPQISLSASFGTGAFTPPNLFSAAGAIWGAGLSIAQPIFHGGELRAQRRAAMENYDA
                     SLAQYRQTVLNAFQNVADTLTALHEDAQALQSARQAADAARASFELADARYRLGAVAY
                     PEQVLAEQQWQNAQQAVIQSRAARLSDTAGLFQAMGSPP"
     gene            1836..2948
                     /locus_tag="VGC69_10365"
     CDS             1836..2948
                     /locus_tag="VGC69_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY0295723.1"
                     /translation="MSLLFTPTPLGPVTLDNRIVIAPMCEYSAENGCASDWHMIHLGH
                     LALSGAALLILEATAVEPIGRISPYDLGLWSDENEAALGKVVQAIRRYSPIKLGVQIS
                     HAGRKASSRAPWEGGQQITVAEGGWRCVAPSAVPHSETEETPLALDEAGLARIKQAFV
                     DTALRAARLGFDVIELHGAHGYLLHQFLSPLANQRTDAYGGSLENRMRFPLDVFKAVR
                     AAVPQAVSVGIRVSATDWVEGGLDTPQVVEFAHALKASGCDFIHVSTAGVSPLQKIPL
                     SAGYQVPFAEIIKREVGMPTITVGLITEAEHAEEIVQKGQADMVALARGILYDPRWPW
                     HAAAKLGAAVDAPRQYWRCQPRELKALFKDAAYGQR"
BASE COUNT          431 a         1065 c         1043 g          441 t
ORIGIN      
        1 ctgggcacga tcgcgatgtt cggcatcatc atgcgcaact cggtcatcct ggtggaccaa
       61 atcgagcagg acatccgcgg cggggcgccg cgctggcaag ccatcatcgg cgccaccgtg
      121 cggcgtttcc gtcccattac gctgaccgcc gccgccgcgg tgctggcgct gattccgctg
      181 ttgcgcagca atttcttcgg gccgatggcg acggccctga tgggaggcat taccgtggcg
      241 acggtgctga cgctgttcta cctgcccgcg ctgtatgccg cgtggttccg cgtgcgggcc
      301 gacgagccgg ccaaggaggc gcagccatga acgccgcgct tgcaatggcc acggccgcgg
      361 gagcggcgag gctcctgaag agagggctcc tgccagccac catggcgctg gcgctggccg
      421 gttgcgcgtt cgaagcgccc accaagcctc ccgcgctgcc cgatcccgcg cgctacaccg
      481 cggcgccgct gccggcggac acggtggcgg ccgacgggac ggcccagcat ttcagcctgg
      541 gcgagcggcc cgtgccgcag tggtggcgcc gctacggcag cgagacgctc gacgcctgga
      601 tcgacgaggg cctgcgcaac agtccgacgc tcgaatcggc cggccatgcc ctggccgccg
      661 cgcgcgagac cttgcgcggg cagatcggcg cggtggagct gccgacggtc gatgtccagg
      721 tgcagaccga gcggcagcgg gccttgggcc tgcctgcctt cggccagccc accaacctgt
      781 acaacgtcta taccggccag ctggcgctga gctacgacct ggacctgtcc ggcgccgcgc
      841 gttacggcat cgcgcaggtc accgcgcagg tcgaccggca atcgttcgag ctcgacacgg
      901 cccgccgcac gctggcggcc aatatcgtga tggcggcgat cagcgcggcc tcggccgatg
      961 cgcagcgcca ggccgccgag cgccttgcgg ccctggcgca cagccggggg gacatcgccg
     1021 cgcgggcccg cgggctcggc gcggcgtcgc cggccgacgt gctgaccgtg cagcaggagg
     1081 cggccagcct cgatgccgcg ttgcccgcgc tgcgcgcgca gacgcagcgg gcgcgccatg
     1141 cgctggcggt gctgatgggg cgctcgcccg accaggcgcc gcccgtgctg ccgctggacg
     1201 acctgcgcct gcccgacgac gtgcccgtga gcgtgccgtc ggagttgctg caccagcgcc
     1261 ccgacgtgct ggcggccgag gccggcgtgc gcatcagcgc cgcccagatc ggcgtggcga
     1321 cggccaatct gtttccgcag atctcgctgt cggcctcgtt cggcacggga gcctttacgc
     1381 cgcccaacct gttttcggcg gcgggggcga tctggggggc cggcctgtcg atcgcgcagc
     1441 ccatcttcca cggcggcgaa ctgcgggcgc agcgccgggc cgccatggaa aactacgatg
     1501 cttccctggc gcaataccgg caaactgtgc tcaatgcctt ccagaacgtc gccgacacgc
     1561 tgacggccct gcacgaggac gcgcaggcgc tgcagtcggc acggcaggcg gccgacgcgg
     1621 cacgggcctc cttcgagctg gcggatgcgc gctaccggct gggcgcggtt gcctatccgg
     1681 agcaggtgct ggccgagcag cagtggcaga acgcgcagca ggccgtgatc caatcgcgcg
     1741 ccgcgcgcct gtccgatacc gcgggactgt tccaggctat gggctcgccg ccctgatcgt
     1801 tgtatgctga cgacgaattt ttccggagtc cttccatgag tctgctgttc accccgaccc
     1861 ccctggggcc tgtcactttg gacaatcgca tcgtcatcgc gccgatgtgc gaatactcgg
     1921 ccgaaaacgg ctgcgccagc gattggcaca tgatccacct gggccacctg gccctgtccg
     1981 gcgcggccct gctgatcctg gaggccacgg cggtcgaacc catcggccgc atcagcccct
     2041 acgacctggg cctgtggagc gacgagaacg aagcggcgct gggcaaggtc gtgcaggcca
     2101 tccgccgcta ttcgcccatc aagctgggcg tgcagatctc gcatgccggg cgcaaggcct
     2161 cgagccgggc gccctgggaa ggcggccagc agatcaccgt ggccgaaggg ggctggcgct
     2221 gcgtggcgcc gtcggccgtg ccgcacagcg agaccgaaga gacgccgctg gccctggacg
     2281 aggccggcct ggcccgcatc aagcaggctt tcgtggacac ggccctgcgc gccgcgcgcc
     2341 tgggcttcga cgtgatcgag ctgcacggcg cgcacggcta cctgctgcac cagttcctgt
     2401 cgccgctggc caaccagcgc accgacgcct atggcggttc gctggagaac cgcatgcgtt
     2461 ttccgctgga cgtgttcaag gcggtgcgcg ccgccgtgcc gcaggcggtg tcggtcggca
     2521 tccgcgtgtc ggccaccgat tgggtcgaag gcggcctgga tacgccgcag gtcgtcgagt
     2581 tcgcccatgc gctcaaggcg tcgggctgcg acttcatcca cgtgtcgacg gccggcgtct
     2641 cgcccttgca gaagatccct ctttcggcgg gctaccaggt cccgttcgcc gagatcatca
     2701 agcgcgaagt cggcatgccc accatcacag tgggtctcat caccgaagcc gaacacgccg
     2761 aggaaatcgt gcagaagggg caggccgaca tggtggccct ggcgcgcggg atactctacg
     2821 atccccgctg gccctggcac gccgcagcca agctgggcgc cgcggtcgac gcgccgcgcc
     2881 agtattggcg ctgccagccg cgcgagctca aggccctgtt caaggacgcc gcctacggcc
     2941 agcgctgagc aagcgtcgat gcgaaacagc ccgcttatcg
//
LOCUS       DASWPO010000199         5030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2187846, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000199 DASWPO010000000
VERSION     DASWPO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187846"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..946
                     /gene="tam"
                     /locus_tag="VGC69_10370"
     CDS             176..946
                     /gene="tam"
                     /locus_tag="VGC69_10370"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEY0295724.1"
                     /translation="MSWSAKQYVTFEDERTRPVRDLLAAIPAHNVRSAVDIGCGPGNS
                     TEILAAHFPDATVSGLDSSGDMIEAARKRMPRVHFDIGSIEAWTGTDPFDVILANAVL
                     QWVPDHVSLLPLLVERLAPGGSLAIQMPDNLEEPAHRVMREIALTGKWAAKLAKAFET
                     RAALPSADWYYELLKKQCVKVDVWRTIYHHPLAGGASAVVEWFKGSALGPFLEPLDGE
                     ERAQYLAEYLKAIDGAYRTLSDGSVLLPFPRLFIVATR"
     gene            complement(927..2153)
                     /locus_tag="VGC69_10375"
     CDS             complement(927..2153)
                     /locus_tag="VGC69_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295725.1"
                     /translation="MPASAPPLSQPMDARPERATLRAWLAVLALAVGCFAFVSSEMLP
                     IGLLGPIAAGLHLPLGVAGLLVTGFAVMVALTAGPMTVLTGRIDRRRLMVLLMLASLA
                     GNALAAVSRDFATLLVARLIVALAIGVFWSIATAMAVRLVPERQAVSATSVVLGGLAV
                     AAVLGVPMGTLIGQYAGWHTAFAALSLLSLLVGMAALLLLPPMPAQSAGSLRAIADAF
                     GHDALRTVFYATALTMTGNFLAYTYVTAYLQDVAGIPSHWMSALLLVYGVAGVAGNFS
                     VGAAMQHSLRGALLGVLALLAASLLLLWAAGTNRIAAIALLVPWGIGYAALPVLLQTW
                     VFRAARETGDAEAALSLFVMAFNAAIALGALLGGLVVDHAGPRAPMLYAGLLVAAAWL
                     LVARSRRTAQISAWPQ"
     gene            complement(2185..3051)
                     /locus_tag="VGC69_10380"
     CDS             complement(2185..3051)
                     /locus_tag="VGC69_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEY0295726.1"
                     /translation="MYAITGITGQVGGALARRLLAQEQTVRAVVRDPAKGRAWTAQGC
                     GLALAQADDVQALTRAFADTQGVFILLPPNFDPAPGFPESRRLIGALHAALLAARPQR
                     VVCLSTVGAQATQTSLLSQLGLLEEALSSLPMPIAFLRAAWFMENALWDVASACDQGC
                     LDSYLQPLDRAIPMVSAADVGRTAAELLQETWTGRRIVELQGPTPVSPRDLAAGFARL
                     LGRDVGTRAVPRERWEALFREQGMANPLPRMQMLDGFNQGWIRFEEDGVQARRGGTAL
                     DAVLRSLLERAR"
     gene            3167..4084
                     /locus_tag="VGC69_10385"
     CDS             3167..4084
                     /locus_tag="VGC69_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295727.1"
                     /translation="MAFDERALNGMGVLAAVVNGGSFAAAADALDMTPSGVSRAVVRL
                     EERLGIRLFDRTTRSVALTDEGRGFYERIVPLLAGLEEAAALASEGQVAVRGRLRVNV
                     DPFFSRLILGPRLGSFLRECPALRVELITREEPGDMIAEGFDLAIRFGEPRPSSLVAR
                     KLLDTRILTVASPAYLKAHGRPAEPRDLPAQGHVCIQFRDPQTGRPFTWDFKRGRKRL
                     ELPLGGPLLVNDVGTLHGACLAGCGMAQVMELGVEHLLAEGRLVNVLPDWSDERFPLY
                     AFYPSRSYLPAKTRAFLDFVLAVVQPPAR"
     gene            4256..>5030
                     /locus_tag="VGC69_10390"
     CDS             4256..>5030
                     /locus_tag="VGC69_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0295728.1"
                     /translation="MTATHSVTEQALLARYGSDALPGPLNDIAAQLLEHRSVRSYADK
                     PVSDEILRVSIAAAQSASTSSNLQVWSVVAVRDRAKLAKLGDLAGGQQHVINAPLFLA
                     WVLDLSRLRRLGDALGVPTKGTDYLEMYTTGAVDVALAAQNAAVALESYGLGLVYIGG
                     MRNHPEDVAELLGLPQGSMVLFGMCVGYPDPALPADIKPRLPQSSVLHFERYDAANET
                     AEVQDYDARMDAFNESQARKTRPWSQLATHRVRGPESLSG"
BASE COUNT          862 a         1588 c         1790 g          790 t
ORIGIN      
        1 acaactgggg gtacttccag cgattcccgc gaagaccggt gacgcaattt ctccgcgaaa
       61 cctagtgttc acagcctttt gccggccgat caacgtttaa acgtgtgatc aacgtctgat
      121 cacagcatca gaaggaagcg gcggcggtac tgccgaatag tcaaaggaac gtctcatgag
      181 ttggtccgcc aagcagtatg tgacattcga agacgaacgt acgcgcccgg tgcgcgacct
      241 gctcgccgca ataccggccc acaacgtccg ctcggccgtg gacataggat gcgggccggg
      301 taattcgacc gagattctcg ccgcgcattt tccggacgcg acggtaagcg ggctcgacag
      361 ctcgggcgac atgatcgagg cggcgcgcaa gcgaatgccg cgcgttcatt tcgacattgg
      421 gagcatcgag gcctggactg gcacagatcc cttcgatgtg atcctggcga acgccgttct
      481 ccagtgggtg cccgaccacg tcagcctgtt gcccttactt gtggagaggc tggcgcccgg
      541 cggcagtctt gccattcaga tgcccgacaa ccttgaggaa ccggcacacc gggtcatgcg
      601 ggaaatagca ttgaccggga aatgggcggc gaagcttgcg aaagcattcg agacgcgcgc
      661 tgcgttgcca agtgccgatt ggtattacga attgctgaag aagcagtgcg tgaaggttga
      721 cgtttggcga acgatttatc accacccgct cgctggcggc gcatccgccg tcgtcgaatg
      781 gttcaagggc agtgccttgg gaccttttct cgagccgctc gatggtgagg agcgggcgca
      841 atacctggcg gaatatctga aggctatcga tggcgcctac aggacgctgt cggatgggtc
      901 ggtgttgttg ccctttcccc gtttgtttat tgtggccacg cgctgatttg cgcagtccgg
      961 cgtgagcgcg ccaccagcag ccaggcggcg gcgaccagaa gccccgcgta cagcattggc
     1021 gcacgcggcc cggcatgatc gacgaccaag ccgcccagca gggcgccgag cgcgatcgcc
     1081 gcgttgaatg ccatgacgaa gagcgaaagc gcggcttcgg cgtcgccggt ctcgcgtgcc
     1141 gctcgaaaaa cccaggtctg cagcagcacg ggcagcgcgg cgtagccgat gccccaaggc
     1201 acgagcaggg cgatggcggc gatgcgattc gtgccggcgg cccagagcag cagcaggctg
     1261 gccgccagga gggcgagcac gcccagcagc gcaccgcgca gtgagtgctg catggcggcg
     1321 ccgaccgaga aattccccgc cacacccgcc acgccgtaga ccagcagcag cgcgctcatc
     1381 cagtgcgacg ggatgccggc gacgtcctgc aagtaggctg tgacgtaggt ataggccagg
     1441 aagttgcccg tcatggtcag ggcggtggcg tagaacacgg tgcgcaaggc gtcatggccg
     1501 aaggcgtcgg caatggcgcg caggctgccg gcgctttgcg cgggcatggg cggcaggagc
     1561 agcagggcag ccatgcccac cagcaggctc agcagcgaca gcgccgcgaa ggcggtgtgc
     1621 cagccggcgt attgaccgat caaggtaccc atcggcacgc ccagcacggc ggccacggcc
     1681 aatccgccca gtacgacgga ggtggcggat acggcctgcc gttcaggcac gagccggaca
     1741 gccatggcgg tggcgatgga ccagaacacg ccgatggcca gcgccacgat caggcgggcg
     1801 acgagcaggg tggcgaagtc ccgggagacg gcggccagcg cgttgccggc caggctggcc
     1861 agcatgagca ggaccatcag ccggcggcgg tcgatgcggc cggtcagcac ggtcatgggg
     1921 ccggccgtca gggcgaccat gacggcgaag ccggtgacaa gcaggccggc gacgcccagc
     1981 ggcaagtgca ggcccgcggc gatcgggccg agcagtccga tgggcagcat ctcggagctg
     2041 acgaaggcga agcagccgac ggccagcgcc aggacggcga gccaggcgcg cagcgtggcg
     2101 cgctccgggc gggcgtccat gggctgggac aagggtgggg cggaagcggg catgagtgat
     2161 cctgtgaaaa aagggcaggg cgggtcagcg cgcccgctca agcaggctgc gcagcacggc
     2221 gtccagggca gtgccgccgc gccgggcctg caccccgtct tcttcgaagc ggatccagcc
     2281 ttggttgaag ccgtcgagca tctgcatgcg cggcaaggga ttggccatgc cctgttcgcg
     2341 aaacagcgct tcccagcgct cacgcggcac ggcgcgggtc ccgacatccc ggcccagcag
     2401 gcgggcaaag cccgcggcca ggtcccgtgg cgagaccggt gtcgggccct gcagctcgac
     2461 gatgcgcctg ccggtccagg tctcttgcag cagctcggcc gcggtgcggc cgacgtcggc
     2521 ggcgctgacc atgggaatgg cacggtccag cggctgcagg tagctgtcga ggcagccctg
     2581 gtcgcaggcg ctggccacat cccacagggc gttttccatg aaccaggccg cgcgcaggaa
     2641 ggcgatgggc atgggcaggc tgctcaatgc ctcttccagc agacccaatt ggctgagcag
     2701 gctggtttgc gtggcctggg cgcccacggt cgagaggcag acgacgcgct gcggccgcgc
     2761 ggcaagcaag gccgcatgca atgcgccgat gaggcggcgc gattcgggaa agccgggcgc
     2821 agggtcgaaa ttgggcggca gcaggatgaa cacgccctgc gtgtccgcga aggcacgggt
     2881 cagggcctgg acatcgtcgg cctgcgccag ggcgaggccg cagccctggg cggtccatgc
     2941 gcggcccttg gcggggtcgc gcacgacggc gcgcacggtt tgttcctggg cgagcagccg
     3001 gcgggcgagg gcgccgccga cttggccggt gattccggtg atggcgtaca tgaagtttcc
     3061 ttgaagaaag acgctatggc agccattctg gattccggta attaatgtgt cgacagcatg
     3121 agtgtccata tagaattgac aaaagatcat caaatagaga gacggcatgg cattcgacga
     3181 acgggcgctc aacggcatgg gggtgctggc ggcggtggtc aacggcggca gctttgccgc
     3241 cgcggccgat gcgctggaca tgacgccttc gggcgtgagc cgcgctgtcg tgcggctgga
     3301 agagcgcctg ggcatacgtc tcttcgaccg gaccacgcgc tcggtggcgc tcaccgacga
     3361 ggggcgcggc ttctacgagc gcatcgtccc gctgctggcc gggctggaag aggccgccgc
     3421 gctggcatcg gaagggcagg tagcggtacg cgggcggctg cgcgtcaatg tggacccgtt
     3481 cttctcccgc ctgatcctgg ggccgcgcct ggggagcttc ctgcgcgagt gcccggcctt
     3541 gcgggtggag ttgatcactc gcgaggaacc gggcgacatg atcgcggagg gcttcgattt
     3601 ggcgatccgc ttcggcgagc cgcggccctc gtcgctggtg gcgcgcaagc tgctcgatac
     3661 gcgcatcctc accgtggcgt cgcccgccta cctcaaggcg catggcaggc cggccgagcc
     3721 tcgggacttg ccggcgcaag gccatgtctg catccagttc cgcgatccgc agaccggccg
     3781 gcccttcacc tgggatttca agcgcggccg caagcggttg gaactgcccc tgggcggtcc
     3841 gctgctggtc aacgacgtgg gtaccctgca tggcgcctgc ctggcgggct gcggcatggc
     3901 gcaggtcatg gaattggggg tggagcacct gctggccgag ggccggctgg tcaatgtatt
     3961 gccggactgg tcggacgagc gctttccgct ctatgcgttc tatccttcgc gcagctactt
     4021 gccggccaag acgcgggcct tcctggattt cgtgctggcc gtcgtccagc cgccggcaag
     4081 gtgaaggcct gaccagcctg ccgatggctg acctgcaggg tcggtctctc ctccggggaa
     4141 agcccgggcc gccgtgcggc ttttcccgta ttccggatgg atcgcgatac gataagggcc
     4201 acgtcggggc aggcttggat ccgctccggt tcagatcaat ataggtgaga cccacatgac
     4261 tgctacccat tccgtcacag aacaggcgct gcttgcccgt tacggcagcg atgcgctgcc
     4321 cggcccgctc aatgacatcg cggcccaatt gctcgagcac cgctcggtgc gcagctatgc
     4381 cgacaagccg gtgagcgacg agatcctgcg cgtgtcgatc gccgccgcgc agtcggcctc
     4441 gacttcgtcc aatctgcagg tatggagcgt cgttgccgtg cgtgaccgcg ccaagctggc
     4501 caagctgggc gatctggccg gcggccagca gcatgtgatc aatgcgccgc tgttcctggc
     4561 ctgggtgctg gacctgtccc gcctgcgccg gctgggcgat gcgctgggcg tgccgaccaa
     4621 gggcacggat tatctggaga tgtacaccac gggtgcggtc gacgtcgccc tggccgcgca
     4681 gaacgccgcc gtggcgctgg agtcgtacgg cctgggcctg gtgtatatcg gcggcatgcg
     4741 caaccatccg gaggatgtag ccgagctgct gggcctgccc cagggcagca tggtgctgtt
     4801 cggcatgtgc gtgggctacc ccgatcccgc cctgcccgcc gacatcaagc cgcgcctgcc
     4861 gcagtcgtcg gtgctgcatt tcgagcgcta tgacgcggcc aacgaaaccg cggaagtgca
     4921 ggactacgac gcgcgcatgg acgctttcaa tgaaagccag gcccgcaaga cgcgcccctg
     4981 gtcgcaactg gcgacgcacc gcgtgcgcgg gccggagtcg ctcagcggcc
//
LOCUS       DASWPO010000200         5545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2545174, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000200 DASWPO010000000
VERSION     DASWPO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545174"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VGC69_10395"
     CDS             <1..553
                     /locus_tag="VGC69_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0295729.1"
                     /translation="MLAAYGDQGLRLIEVDKSDLGDLSDIVELVAGRPERYIVFCDDL
                     SFEEGEAGYKALKSVLDGSVSSAGENVLIYATSNRRHLMPEYMSENLQATHGADGEIH
                     PGETVEEKISLSERFGLWLSFHPFKQDDYLDIVHHWLRELGCPAGQIDASRAEALQWA
                     LERGSRSGRVAYQFARDWAARHD"
     gene            606..1613
                     /locus_tag="VGC69_10400"
     CDS             606..1613
                     /locus_tag="VGC69_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEY0295730.1"
                     /translation="MSEHKIIDVAAGLILRDDGQLLLGQRPEGKPWPGWWELPGGKLE
                     PGETVLQALARELFEEIGIRVTQAWPWVTYVHRYPNSTVRLAFCQVTAWEGEPSGLEN
                     QQLRWVDPARALADALNQGEDLDPAQPNGRVLPATVPPLRWLQLPTTYGISSIGAPQR
                     LPAFLQRLDAALAGGLKLVQFREPGWPQGSQAESLHAALLAVRERCRAAGAKLLVNGA
                     HPASWWREADGVHLRAPDAAALQSRPALPDGALVGVSAHGHAEIAHARAIGADFTVLG
                     PVLETPSHPGEPGLGWAGFAAGLRDAGLPVFALGGQTEATRKIAREHGAHGIAGIRAI
                     I"
     gene            1621..2070
                     /locus_tag="VGC69_10405"
     CDS             1621..2070
                     /locus_tag="VGC69_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HEY0295731.1"
                     /translation="MNTTAPNLTLGDPRLDQDHAELHRLVQQLAQARNREEVAAALDA
                     LAGHCLDHFATEDEDLHLMQGNDDAVCHIDEHAAVLKSLAEVRAWLEQNEDVVQMVNV
                     VQSLCAELERWLPEHVQYMDAAVAAYRTKNRFGGVPIVFPSRLPKAQ"
     gene            complement(2033..3532)
                     /locus_tag="VGC69_10410"
     CDS             complement(2033..3532)
                     /locus_tag="VGC69_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0295732.1"
                     /translation="MKAMPPIPSLSISGRAARGALGLSLCALLGACAVGPDYHRPDAP
                     VAASYKELAGWRPASPDAELAPKGEWWRAFHDPLLDELEPQVIVSNQTVRQSYANYQQ
                     ALAEVRVAQSSLFPTIGLTGSATRQRSNGGTSLGGASRVQSSGSLEGSADWELDIWGR
                     IRRTIEENKAVAQADEALLASAALSEQIALANAVVELRTADADIDLLQRTVSAYEASL
                     RVVENQSRAGTAAPSDLMTALTQHDSARASLISLGVARAQYEHAIAVLVGKNPAELAI
                     AHNGRLPDLPEVPVGLPSELLQRRPDIAAAERQMAAQNAAIGVAVAAYYPTISLSGLL
                     GFTQSPLSGLLNAANRVWSLGADANATLFDGGARSGAVRAAQAAYDAEVANYRGTVLA
                     AFQGVENDLSGLRILAQQAEAQRAAVDHATQGARITLNEYQAGTVDYTTVATTQATLL
                     SNQQSLLNVESQRRLDAVSLIGDLGGGWEGLKEPAAEATAPSASATEKQ"
     gene            complement(3532..>5545)
                     /locus_tag="VGC69_10415"
     CDS             complement(3532..>5545)
                     /locus_tag="VGC69_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0295733.1"
                     /translation="AEVSFTVISMSLSLIAVFLPILIMPGVLGLMFHEFAVTLSIAIL
                     ISLVVSLTVTPAMCAYLLSRENSGHSPSRWARWVEAGFERFKHAYDRSLAVALDHSFL
                     VILVLVGLLVSNLFLFRLVPATFFPEQDNGLLMGSIRADQSISFPSMEKKLAQLQQIV
                     KQDPAVESVAGFTGGRALNSATSFIQLKPLAERGVSAQEVANRLRPKLDQVAGARLFL
                     QAQQDLRIGGRQSASEYQYTLTSDDPDTIITWTPKLIDELNKHPEALTDVNSDLQQHG
                     LQTYLTINRATAMRYGFQPNQIDSVLYDAFGQRTVSTIYNALNQYFVVMEVAPQYWQY
                     PQMLDQIYLSTAAGNANGSAQTQMSGALVPKAAKQAAAASNNTNARNTDAATNQATNS
                     ISNSKGSSSSGSADSTAAETMVPLSALASWATSHTATQVNHQSGLVAGTISFNLPPGG
                     SLSTADKAINDALSEIRAPTTLHGSFAGSARTYAQSMGTLPLLVAAAIGVVYLVLGIL
                     YENTIHPLTILSTLPSAGIGATLALLVFGTPFSVIALIGMFLLIGIVKKNGIMMVDVA
                     IQLQRADGLDARTAIHRAAVMRLRPIMMTTAAAVLGAVPLAIGIGQGAALRQPLGITV
                     MGGLVLSQVFTLYTTPVIYLYLDRLRARIAVWSARLPWSRQPDTTS"
BASE COUNT          872 a         1918 c         1921 g          834 t
ORIGIN      
        1 catgctggcc gcctacggcg accagggcct gcgcctgatc gaagtcgaca agtccgacct
       61 gggcgacctg tccgacatcg tcgaactggt cgccggccgg cccgagcgct acatcgtctt
      121 ctgcgacgac ctctccttcg aagagggcga ggccggctac aaggcgctga agtccgtgct
      181 cgacggctcg gtctcctcgg ccggcgaaaa cgtgctgatc tacgccacct ccaaccggcg
      241 ccacctgatg ccggaataca tgagcgagaa cctgcaggcc acgcacggcg ccgacggcga
      301 gatccacccc ggcgaaaccg tcgaggaaaa aatctcgctg tccgagcgct tcggcctgtg
      361 gctgtccttc caccccttca agcaggacga ctacctcgac atcgtccacc actggctgcg
      421 cgagctgggc tgcccggccg gccagatcga cgcctcgcgc gccgaagccc tgcaatgggc
      481 cctggagcgc ggctcgcgtt cgggccgcgt ggcctaccag ttcgcccgcg actgggcggc
      541 gcgccacgat tgaacccggg gcgggcccga cgccggcccg ccccttgcca catacaccgc
      601 aagccatgtc cgaacacaag atcatcgacg tcgccgccgg cctcatcctg cgcgatgacg
      661 gccagttgct gctgggacag cgccccgagg gcaagccctg gccgggctgg tgggaactgc
      721 ccggcggcaa gctcgaaccc ggcgaaaccg tgctgcaggc cctggcccgc gagctgttcg
      781 aggaaatcgg catccgcgtc acccaggcct ggccctgggt gacctatgtg caccgctacc
      841 ccaacagcac cgtgcggctg gccttctgcc aggtcacggc ctgggaaggc gagccgagcg
      901 gcctggagaa ccagcaactg cgctgggtcg acccggcgcg ggccctggcc gacgcgctga
      961 accagggcga ggacctcgat cccgcccagc ccaacggccg ggtgctgccc gccaccgtcc
     1021 cgccgttgcg ctggttgcaa ctgcccacca cctacggcat ctcgtcgatc ggcgcgccgc
     1081 aacggctgcc cgcttttctg caacggctcg acgcggccct ggccggcggc ctgaaactgg
     1141 tgcagttccg cgaaccgggc tggccgcaag gcagccaggc cgagtcgctg cacgccgcct
     1201 tgctggccgt gcgcgagcgc tgccgcgccg ccggcgcgaa gctgctggtc aacggcgcgc
     1261 acccggcatc gtggtggcgg gaagccgacg gcgtgcacct gcgcgcgccg gatgccgcgg
     1321 cgctgcaaag ccgcccggcc ctgcccgacg gcgcgctggt cggcgtctcc gcccatgggc
     1381 acgccgagat cgcgcacgcc cgcgccatcg gcgccgactt caccgtgctg ggcccggtgc
     1441 tcgaaacgcc ttcgcacccg ggcgaaccgg gcctgggctg ggcgggtttt gccgcgggcc
     1501 tgcgcgatgc gggactgccc gtcttcgccc tgggcgggca aaccgaagcc acgcgtaaaa
     1561 tcgcccgtga acacggcgcg cacggcatcg ccggcatacg cgcgataatc taggcacacc
     1621 atgaatacca ccgcacccaa cctcaccctg ggcgacccgc ggctcgacca ggaccacgcc
     1681 gagctgcatc gcctggtgca gcagctcgcg caagcccgca accgggaaga ggtcgccgcc
     1741 gccctggacg cgctggccgg gcactgcctc gaccacttcg ccaccgagga cgaagacctg
     1801 cacctcatgc agggcaacga cgacgccgtc tgccatatcg acgagcacgc cgcggtgctg
     1861 aagtccctgg ccgaggtcag ggcctggctc gagcagaacg aagacgtcgt ccagatggtg
     1921 aacgtggtgc agtcgctatg cgccgagctg gagcgctggc tgcccgagca cgtgcaatac
     1981 atggacgcgg ccgtggccgc ctatcgcacc aagaaccgct tcggcggcgt gcctattgtt
     2041 tttccgtcgc gcttgccgaa ggcgcagtag cctcggccgc cggctctttc aagccttccc
     2101 atccgccgcc caggtcgccg atcagcgaga cggcgtcgag ccggcgctgc gactcgacgt
     2161 tcagcagcga ctgctggttg ctgagcaggg tggcctgcgt cgtggccacg gtggtgtagt
     2221 cgaccgtgcc cgcctggtac tcgttgagcg tgatgcgcgc gccctgggtg gcgtgatcca
     2281 ccgcggcgcg ctgcgcctcg gcctgctgcg cgagaatgcg caagccggac aggtcgttct
     2341 cgacgccctg gaaggcggcc agcaccgtgc cgcggtagtt ggccacctcc gcatcgtagg
     2401 cggcctgcgc cgcgcggacc gcgccgctgc gcgcgccgcc gtcaaagagc gtcgcgttcg
     2461 cgtcggcgcc cagcgaccag acgcggttgg ccgcgttcag caggccggac agcggcgact
     2521 gcgtaaagcc gagcagcccg gacagcgaga tcgtcgggta gtaggcggcc acagccacgc
     2581 cgatcgccgc gttctgcgca gccatctggc gctccgccgc cgcgatatcg gggcgccgct
     2641 gcagcagctc cgaaggcagg ccgacgggca cctcgggcag atccggcaag cggccgttat
     2701 gggcaatggc gagctccgcg ggattcttgc cgaccagcac ggcaatggca tgctcgtatt
     2761 gcgcgcgcgc cacgcccagc gagatcaggc tggcccgcgc gctgtcgtgc tgggtgagcg
     2821 cggtcatcag gtccgacggc gcggccgtgc cggcgcggct ctggttctcg accacgcgca
     2881 gggacgcctc gtaggcggag acggtccgtt gcagcaggtc gatatcggca tcggccgtgc
     2941 gcagctcgac gaccgcgttg gccagcgcga tctgctcgga cagggccgcg ctggccagca
     3001 aggcctcgtc ggcctgcgcg accgccttgt tttcctcgat ggtgcggcgt atccggcccc
     3061 agatgtcgag ctcccagtcc gcgctgcctt ccagcgagcc ggagctctgc acgcgcgacg
     3121 cgccgcccag ggacgtcccg ccgttgctgc gctgccgggt ggccgagccg gtcaggccta
     3181 tcgtgggaaa gagcgagctt tgcgcgaccc gcacctcggc cagcgcctgc tggtagttgg
     3241 cgtagctttg gcgcacggtc tggttggaca cgatgacctg cggttccagc tcatcgagca
     3301 gcgggtcgtg gaacgcgcgc caccattcgc ccttgggcgc cagctccgcg tcgggggagg
     3361 caggccgcca gcccgccagt tccttgtagg aggccgcgac cggggcatcg ggacggtggt
     3421 agtccggacc cacggcgcac gcgcccagca gcgcgcaaag cgacaggccc agcgcgccgc
     3481 gcgccgcgcg gccggaaatg gacaaggacg ggataggagg catggctttc atcatgaagt
     3541 cgtgtccggt tgccggctcc agggcaggcg cgccgaccag accgcgatcc gggcgcgcaa
     3601 acggtcgagg tacaaataaa tgaccggcgt cgtgtagagc gtaaacacct ggctcagcac
     3661 cagcccgccc atcacggtga tcccgagcgg ctggcgcagc gcggcgccct ggccgatgcc
     3721 gatcgccagc ggcaccgcgc cgagcacggc ggccgcggtg gtcatcatga tggggcgcag
     3781 ccgcatgacg gcggcccggt ggatcgccgt gcgcgcgtcg aggccgtcgg cgcgctgcaa
     3841 ctggatcgcg acgtcgacca tcatgatgcc gtttttcttg acgattccga tcagcaggaa
     3901 catgccgatc agggcgatca ccgagaaagg cgtgccgaac accagcaggg ccagcgtggc
     3961 gccgatcccg gccgagggca gcgtggaaag aatcgtcagc gggtggatgg tgttctcgta
     4021 cagaatgccc agcaccaggt agaccacccc gatggccgcg gcgaccagca agggcagcgt
     4081 gcccatggat tgcgcatacg tgcgcgccga gccggcaaag gagccatgca gcgtggtcgg
     4141 cgccctgatt tcgctcagcg catcgttgat ggccttgtcc gccgtgctca gcgagccgcc
     4201 cggcggcagg ttgaacgaga tcgttcccgc caccaggccg ctctggtggt tgacctgcgt
     4261 ggccgtatgg ctggtggccc agctcgcgag cgccgagagc ggcaccatgg tctcggccgc
     4321 ggtgctgtcg gcgctgccgc tcgaggagct gcccttgctg ttggaaatgc tgttggtcgc
     4381 ctggttggtc gccgcgtcgg tgttgcgcgc gttggtgttg ttcgacgcgg cggccgcctg
     4441 cttcgcggcc ttgggcacca gcgcgcccga catctgcgtc tgcgccgagc cgttggcgtt
     4501 gcccgccgcg gtgctgaggt aaatctggtc cagcatctgc gggtattgcc aatattgcgg
     4561 cgccacttcc atgacgacga aatactggtt gagcgcgttg tagatggtcg agaccgtgcg
     4621 ctggccgaag gcatcgtaca gcacgctgtc gatctggttg ggctgaaaac cgtagcgcat
     4681 ggcggtcgcc cggttgatcg tcaggtaggt ctgcaggccg tgctgctgca ggtccgagtt
     4741 gacgtcggtc agcgcctcgg gatgcttgtt gagctcgtcg atcagcttcg gcgtccacgt
     4801 gatgatggtg tccgggtcgt cgctggtcag ggtgtactgg tattcggagg ccgattgccg
     4861 cccgccgatg cgcaggtcct gctgcgcctg caggaacagg cgcgcgccgg cgacctggtc
     4921 gagcttgggc cgcagccggt tggcgacttc ctgggcggac acgccgcgtt ccgcgagcgg
     4981 cttgagctgg atgaaggacg tcgccgagtt gagcgcccgc ccgccggtga agcccgccac
     5041 cgactcgacg gccgggtcct gcttgacgat ctgctgcagc tgggcgagct tcttttccat
     5101 ggacgggaag gaaatgctct ggtcggccct gatcgagccc atcagcaagc cgttgtcctg
     5161 ttccgggaag aaagtcgccg gcaccaggcg gaacaggaac aggttcgaga ccagcaggcc
     5221 gaccagcacc aggatcacca ggaacgaatg gtcgagcgcc accgcgagcg agcggtcgta
     5281 ggcatgcttg aagcgctcga accccgcttc gacccagcgc gcccagcgcg acggcgaatg
     5341 gccgctgttt tcgcggctca gcaagtaggc gcacatggcc ggcgtgacgg taagcgacac
     5401 gaccagcgag atcaggatgg cgatcgacag ggtgaccgcg aactcgtgga acatcaggcc
     5461 gagcaccccc ggcatgatca ggatgggcag gaacacggcg atcagcgaca ggctcatcga
     5521 gatcacggtg aagctcacct ccgcg
//
LOCUS       DASWPO010000201         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1832246, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000201 DASWPO010000000
VERSION     DASWPO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832246"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /gene="coaBC"
                     /locus_tag="VGC69_10420"
     CDS             <1..918
                     /gene="coaBC"
                     /locus_tag="VGC69_10420"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY0295734.1"
                     /translation="FMAKLAHGLADDLLSTLCLARACPLLVAPAMNREMWANPATQRN
                     VAQLQADGIAILGPGSGSQACGEIGDGRMLEPHQLLAEMIAFFQPKVLAGRHVLLTAG
                     PTAEPIDPIRVLSNRSSGKTGYALARAAQEAGARVTLVSGPTALPAPYGVTLLPVTTA
                     RQMHAAVMAEAADADLFIAVAAVADWRVKNASTHKIKKTGDGAGAPRLEFEANPDILA
                     DVARLPKGPWCVGFAAETENLAQYAEDKRRRKNIPLLVGNLAQNVMDAEATELVLFDA
                     QGMHPLPAAPKLDAARRLIAEIAARLPRG"
     gene            942..1208
                     /locus_tag="VGC69_10425"
     CDS             942..1208
                     /locus_tag="VGC69_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295735.1"
                     /translation="MPEPRIPTSTSPAAKRRLSIVLFVLAVVALAQAVVQLRDALGHA
                     PRAIDDFIMPAFLLIFGAMLLAAARAARRPDTQLTIPPEERPLP"
     gene            1212..1661
                     /gene="dut"
                     /locus_tag="VGC69_10430"
     CDS             1212..1661
                     /gene="dut"
                     /locus_tag="VGC69_10430"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY0295736.1"
                     /translation="MKSVDLKILDARMRDALPTYATPGSAGLDLRACLDEPLEIAAGA
                     TVLVPTGLAIHIADPGYAALILPRSGLGHKHGIVLGNLVGLIDADYQGQLMISTWNRG
                     GTPFTLNPMERLAQLVIVPVQQVSFNVVEEFGDSERGEGGFGSTGRK"
     gene            complement(1726..>1834)
                     /locus_tag="VGC69_10435"
     CDS             complement(1726..>1834)
                     /locus_tag="VGC69_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004202890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY0295737.1"
                     /translation="AAAKLGAAVDAPRQYWRCQPRELKALFKDAAYGQR"
BASE COUNT          285 a          712 c          578 g          259 t
ORIGIN      
        1 ttcatggcga agctggcgca cggcctggcc gacgacctgc tgtccacgct atgcctggcg
       61 cgcgcctgtc cccttctggt ggcgcccgcg atgaaccgcg agatgtgggc caacccggcc
      121 acgcagcgca atgtggcgca gttgcaggcc gacggcatcg ccatcctggg cccgggctcg
      181 ggcagccagg cctgcggcga aatcggcgac ggccgcatgc tcgaaccgca ccagttgctg
      241 gccgaaatga tcgccttctt ccagcccaag gtgctggccg gcaggcacgt gctgctcacg
      301 gcaggcccca ccgctgaacc catcgacccg atacgcgtgc tgagcaaccg ctcctcgggc
      361 aagacgggtt atgcgctggc gcgcgccgcg caggaggccg gcgcccgcgt cacgctggtg
      421 agcggcccca cggccctgcc cgcgccctac ggcgtcaccc tgctgcccgt gacgacggcg
      481 cggcagatgc atgccgccgt gatggccgaa gcggcagacg ccgacctctt catcgccgtg
      541 gccgccgtgg ccgactggcg cgtgaaaaac gccagcacgc acaagatcaa gaaaaccggc
      601 gacggcgcgg gcgccccccg gctggaattc gaagccaacc ccgacatcct ggccgacgtg
      661 gcccgcctgc ccaagggccc ctggtgcgtg ggctttgccg ccgaaaccga aaacctcgcc
      721 cagtacgccg aggacaagcg ccgccgcaag aatatcccgc tgctggtggg caacctcgcc
      781 cagaacgtca tggacgccga ggccaccgaa ctggtgctct tcgacgccca gggcatgcat
      841 ccgctgccgg ccgcccccaa gctcgacgcc gcccgccggc ttatcgccga aatcgccgcg
      901 cggctgccca ggggctgacc gcgcatcgcc cgcgagccct catgcccgag ccccggattc
      961 cgacctcgac ctcgcccgcc gccaagcggc gcctgtccat cgtgctgttc gtcctcgccg
     1021 tcgtcgcgct ggcccaggcc gtggtccagc tgcgcgatgc gctcgggcac gcgccccggg
     1081 cgatcgacga cttcatcatg ccggccttcc tgctgatctt cggcgcgatg ctgctggccg
     1141 ccgcgcgcgc cgcgcgccgc cccgacaccc aactgaccat tcctcccgaa gaacgtcccc
     1201 ttccctgacc catgaaatcc gtcgacctca agatcctcga tgcgcgcatg cgcgatgccc
     1261 tgcccaccta cgccacgccc ggctcggccg gcctggactt gcgcgcctgc ctggacgaac
     1321 ccctcgagat cgctgccggc gccaccgtgc tggtgcccac cggcctggcc atccacatcg
     1381 ccgaccccgg ctacgcggcg ctcatcctgc cgcgctcggg cctgggacac aagcatggca
     1441 tcgtactggg caacctggtc ggcctgatcg acgccgacta ccagggccag ctcatgatct
     1501 ccacctggaa ccgcggcggc acgcccttca cgctcaaccc catggagcgc ctggcccagt
     1561 tggtgatcgt gcccgtccag caagtgagct tcaacgtggt cgaggagttc ggcgactcgg
     1621 aacgcggcga aggcggcttc ggcagcaccg gccgcaaata ggcatggcct gccgcaaaag
     1681 aaaacagccc gctcgataag cgggctgttt cgcatcgacg cttgctcagc gctggccgta
     1741 ggcggcgtcc ttgaacaggg ccttgagctc gcgcggctgg cagcgccaat actggcgcgg
     1801 cgcgtcgacc gcggcgccca gcttggctgc ggcg
//
LOCUS       DASWPO010000202         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2455220, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000202 DASWPO010000000
VERSION     DASWPO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2455220"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /gene="aspS"
                     /locus_tag="VGC69_10440"
     CDS             <1..1465
                     /gene="aspS"
                     /locus_tag="VGC69_10440"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY0295738.1"
                     /translation="FQLDDDNLSETTRLTHRVLDLRRPQMQNNLKLRYRVSIEVRKYL
                     DSLGFIDIETPMLTKSTPEGARDYLVPSRVNPGHFFALPQSPQLFKQLLMVSGFDRYY
                     QITKCFRDEDLRADRQPEFTQIDCETSFLNEVEIREIFEGMIRHVFKVVKDVDLPTPF
                     PQMTWIEAMARYGSDKPDLRVDLEFTDVADIMADVDFKVFASAATTPGSRVVALRVPK
                     GGELTRSEIDAYTQFVGIYGAKGLAYIKVNEVAKGRDGLQSPIVKNLHDAALAELVRR
                     TGAQDGDLIFFGADRVKVVNDAIGALRIKIGHSEFGKRTGLFTSAWKPLWVIDFPMFE
                     YDEEEGRYTAAHHPFTSPKDGHEDFLETDPGQAYAKAYDMVLNGWEIGGGSVRIHREE
                     VQSKVFRALKIGAEEARQKFGFLLDALQYGAPPHGGLAFGLDRIVTMMTGAESIRDVI
                     AFPKTQRAQDLLTQAPSEVDEKQLRELHIRLRNVEVK"
     gene            1561..2274
                     /locus_tag="VGC69_10445"
     CDS             1561..2274
                     /locus_tag="VGC69_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0295739.1"
                     /translation="MTRLRVVTYNIHKGRSALGLRESLNGLRPALHGLHPDLVFLQEV
                     QGRNENISLFDAQHEALAATLRLHMVYGCNAVRHCTDHGNAFLSRYPILRHENQDISD
                     HRLEQRGLLHALIEMEGTQVHCFVVHLGLFAAGRARQAQALADRMRAYVPDDAPILVA
                     GDFNDWGNKLTPFFTRGLGLSDVFAQAPRTFPAAFPWLRLDRIYQRGFAVHGARVLHG
                     PEWARLSDHAPLLAELELR"
     gene            2297..3559
                     /gene="clsB"
                     /locus_tag="VGC69_10450"
     CDS             2297..3559
                     /gene="clsB"
                     /locus_tag="VGC69_10450"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEY0295740.1"
                     /translation="MKDEDLAFLTWTNGNAMHLLQSGADFFPALCAAIDGAQHSVHLE
                     TYIFALDRTGYRVLDSLMAAARRGVQVCLVLDGFGSSTTIAAVRAGLDAAGGQCLVFR
                     PEPRWWGKLIPSRNRLRRLHRKTAVIDGAIAFVGGINIEDDYDDLDPAEGARTPRFDF
                     SVEIRGPLVADVIYAQDLMWVRLNWSRLRRNPGELRDWPRMRLLVPHHVDIAEVSDGD
                     TGQPRPSQYKHLRAALVLRDNVRFRQTFERAYLCCIRDAEREILIANAYFFPGRALRK
                     ALLEAAERGVRVRILLQGKSTHHIQYHATRVLYDHFLAGGIEIHEYQAGYIHAKAAVI
                     DDIATVGSSNLDPFSLLLAREANVMIDDEGFAADLKARVEQGITQGSVSIQQQEFRHR
                     GWLRRGIDNVSYALLRIGVALTGQSRSF"
     gene            complement(3572..3997)
                     /locus_tag="VGC69_10455"
     CDS             complement(3572..3997)
                     /locus_tag="VGC69_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0295741.1"
                     /translation="MSYLLDTNVLSELRRRKPDAQVVQWMKERPATTLYLSVLTLGEL
                     RKGIEALPDSSRKRLLLDWLEIDLPGFFTGRVLPVDVRVANRWGHLLAQAKRPLPAID
                     SLLAATALTHGLTLVTHNLRDFQHPGLEIIDPWQAAGTL"
     gene            complement(3994..4245)
                     /locus_tag="VGC69_10460"
     CDS             complement(3994..4245)
                     /locus_tag="VGC69_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY0295742.1"
                     /translation="MHAWQMQEAKSRLSEVVKEASREGPQEITVHGRPVAVVLSRHDY
                     DQLAGTGESLVAFMRRSPLAQVDDLDFTRDASPTREVGF"
     gene            complement(4351..>4516)
                     /locus_tag="VGC69_10465"
     CDS             complement(4351..>4516)
                     /locus_tag="VGC69_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0295743.1"
                     /translation="GATSVTFLMPVFGMVWGLLLLGEPITQRILLGACVILAGTALAM
                     GLVGPRRAAD"
BASE COUNT          798 a         1565 c         1386 g          767 t
ORIGIN      
        1 gttccagctc gacgacgaca acctgtcgga aaccacgcgc ctgacgcacc gggtgctgga
       61 tctgcgtcgc ccgcagatgc agaacaacct gaagctgcgt tatcgcgtgt cgatcgaagt
      121 gcgcaagtac ctcgactcgc tgggcttcat cgacatcgaa acgccgatgc tgaccaagag
      181 cacgcccgaa ggcgcgcgcg actacctggt gccctcgcgc gtgaacccgg gccacttctt
      241 cgcgctgccg cagtcgccgc agcttttcaa gcagttgctg atggtctcgg gcttcgaccg
      301 ctactaccag atcaccaagt gcttccgcga cgaagacctg cgcgccgacc gccagcccga
      361 attcacccag atcgactgcg agacctcctt tctcaatgaa gtcgagatcc gcgagatttt
      421 cgagggcatg atccgccacg tcttcaaggt ggtgaaggac gtcgacctgc ccacgccttt
      481 cccgcagatg acctggatcg aggccatggc gcgctacggc tccgacaagc ccgacctgcg
      541 cgtcgacctg gagttcaccg atgttgccga catcatggcc gacgtcgact tcaaggtgtt
      601 tgcctcggcc gccaccacgc cgggcagccg cgtcgtggcc ctgcgcgtgc caaagggcgg
      661 cgagcttacc cgcagtgaga tcgacgccta cacccagttc gtcggcatct acggcgccaa
      721 gggcctggcc tatatcaagg tcaatgaagt ggccaagggc cgcgacggcc tgcaatcgcc
      781 catcgtcaag aacctccacg acgccgcgct ggccgagctc gtccgccgca ccggcgcgca
      841 ggacggcgac ctgatcttct tcggcgccga ccgtgtcaag gtcgtcaacg acgccatcgg
      901 cgcgctgcgc atcaagatcg gccacagcga attcggcaag aggacgggcc tgttcaccag
      961 cgcctggaag ccgttgtggg tcatcgactt tcccatgttc gagtacgacg aggaagaagg
     1021 ccgctacacc gccgcccacc accccttcac cagccccaag gacggccacg aggatttcct
     1081 cgagaccgat ccgggccagg cctacgccaa ggcctacgac atggtgctca acggctggga
     1141 aatcggcggc ggctcggttc gtatccatcg cgaggaagtg cagagcaagg tgttccgcgc
     1201 gctgaagatc ggcgccgagg aggcccgcca gaagttcggc ttcctgctcg acgcgctgca
     1261 gtacggcgcg ccgccgcacg gcggcctggc cttcggcctg gaccgcatcg tcaccatgat
     1321 gaccggcgcc gaatcgatcc gcgacgtgat cgccttcccc aagacccagc gcgcgcaaga
     1381 cctgctcacc caggcgccgt ccgaggtcga cgagaagcag ctgcgcgaac tgcacatccg
     1441 tctgcgcaac gtcgaagtca aataggcccc cggctcacac cgcgtgttcg ccgacctccg
     1501 ggagggcaac cgtgccgccc ggcttggggc ggcccggcgc cgggcccggg tggcacaacc
     1561 atgactcgcc tgcgcgtcgt cacctacaac atccacaagg gccgttcggc cctgggcctg
     1621 cgcgagtccc tgaacgggct gcggccggcc ctccacggcc tgcatcccga cctggtcttt
     1681 ctgcaggaag tccaggggcg caacgaaaac atctcgctgt tcgacgcgca gcacgaagcc
     1741 ctggccgcca ccctgcgcct gcacatggtc tacgggtgca atgccgtgcg gcactgcacc
     1801 gaccacggca atgccttcct gtcgcgctac cccatcctgc gccacgaaaa ccaggacatc
     1861 tccgaccacc ggctggagca gcgcggcctg ctgcatgccc tgatcgaaat ggagggcacc
     1921 caggtgcatt gcttcgtggt gcacctgggc ttgttcgccg ccggccgcgc ccgccaggcg
     1981 caggccctgg ccgatcgcat gcgcgcctac gtgcccgacg atgcgcccat cctggtggcg
     2041 ggggatttca acgactgggg caacaagctc acgcccttct ttacccgcgg gctgggcctg
     2101 tccgacgtgt ttgcccaggc gccgcgcacc tttccggcgg cgtttccctg gctgcgcctg
     2161 gaccgcatct accagcgcgg ttttgccgtg cacggcgccc gcgtcttgca tggtcctgaa
     2221 tgggcgcggc tatccgacca cgctcccttg ctggcggagt tggagctgag atagccccga
     2281 cgttatgctc tggcccatga aagacgaaga tctcgccttc ctgacctgga ccaacggcaa
     2341 cgccatgcac ttgctgcaga gcggggcgga cttttttccc gccctgtgcg ccgccatcga
     2401 cggcgcgcag cacagcgtgc atctcgaaac ctacatcttt gccctggacc gcaccgggta
     2461 tcgcgtgctc gacagtctta tggccgcggc ccggcgcggc gtgcaggtct gcctggtgct
     2521 ggatggattc ggcagcagca cgaccatcgc ggccgtgcgc gccggcctgg acgcggccgg
     2581 cggccagtgc ctggtgtttc gccccgagcc gcgctggtgg ggcaagctga tcccgtcgcg
     2641 caaccgcctg cgccgcctgc atcgcaagac ggccgtgatc gacggcgcca tcgcgttcgt
     2701 cggcggcatc aatatcgagg acgactacga tgatctcgac cccgccgagg gcgcgcggac
     2761 gccgcgcttc gatttctccg tcgagatccg cggcccgctg gttgccgacg tgatctacgc
     2821 gcaagacctg atgtgggtgc gcctgaattg gtcgcgcctg cgccgcaatc ccggcgaact
     2881 gcgcgactgg ccgcgcatgc ggctgctcgt gccgcaccac gtcgatatcg ccgaagtgtc
     2941 cgacggcgac acgggccagc cccggccttc gcagtacaag cacctgcgcg ccgccttggt
     3001 tctgcgcgac aacgtgcgct ttcggcagac cttcgagcgg gcctatctgt gctgcatccg
     3061 tgacgcggag cgtgaaatcc tcatcgccaa cgcctatttc tttccgggtc gggccttgcg
     3121 caaggccttg ctggaggcgg ccgagcgcgg cgtgcgggtg cgcatcctgc tgcaaggcaa
     3181 gtccacccat cacatccagt accacgccac tcgcgtgctg tacgaccatt tcctggccgg
     3241 cggcatcgaa atccacgaat accaggcggg ctatatccac gccaaggccg ccgtcatcga
     3301 cgacatcgcc acggtgggct cgtccaatct cgatcccttc agcctgctgc tggcgcgcga
     3361 ggccaatgtc atgatcgacg acgaaggctt cgccgcggac ctgaaggccc gcgtggaaca
     3421 gggcattacc cagggctccg tcagcatcca gcagcaggag ttccgccatc gcgggtggtt
     3481 gcgccgcggc atcgacaatg tgtcgtatgc cctgctgcgc atcggcgtgg ccctgaccgg
     3541 gcagtcacgc tcgttttgag gggctggttt tctatagcgt cccggctgct tgccaaggat
     3601 cgattatctc aagcccgggg tgctggaaat cgcgcaaatt gtgggtcacc agcgtcaagc
     3661 catgcgtcag ggccgtggcc gccaacaagc tgtcgatggc tggcaggggg cgtttcgcct
     3721 gggcaagcaa atgcccccaa cggttggcga cgcgcacatc aacaggcagc acacgcccgg
     3781 taaagaaccc gggcaggtcg atttccagcc agtccagcag caggcgtttg cgagaactgt
     3841 ccggcaaggc ctcaatgccc ttgcgcaatt ctcccagcgt cagcaccgat aggtagagcg
     3901 tcgtggcagg gcgttctttc atccattgca cgacctgggc gtcaggtttg cgccggcgca
     3961 actccgacag cacattggtg tcgagcaaat aactcaaaaa cctacctcgc gcgtcgggct
     4021 ggcgtcgcgt gtgaagtcaa gatcgtccac ctgggccagc ggagaacggc gcatgaaggc
     4081 aaccagggat tcgccggttc cggccaactg gtcgtagtcg tggcgagaca gcaccaccgc
     4141 taccgggcgg ccatgcacgg tgatttcttg cgggccttcg cggctggctt ccttgacgac
     4201 ctccgagagc cgcgacttgg cctcttgcat ttgccaggca tgcatgatgt ccccaataaa
     4261 tctgactaga atagtcagat tttaatcgac tttgcacgca agcgccaaga cgcaaatcca
     4321 cctatccaaa atcgtaggtg gccgaaaagt tcagtcggct gcccggcgcg gcccgaccag
     4381 ccccatggca agcgccgtac ccgccaggat gacgcacgcg ccgagcagga tgcgctgcgt
     4441 gatcggctcg cccagcagca gcaggcccca caccatgccg aacaccggca tcaggaaggt
     4501 cacgctggtg gcgccg
//
LOCUS       DASWPO010000203        20344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1296335, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000203 DASWPO010000000
VERSION     DASWPO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20344
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296335"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1530
                     /locus_tag="VGC69_10470"
     CDS             121..1530
                     /locus_tag="VGC69_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0295744.1"
                     /translation="MNAPQHPETLRRPVPQACLDALRARFGERFSVAEAVREHHGKDE
                     SPYLPVPPDAVVFAHSTDEVAAVARLCNEHRVPLIPYGTGSSLEGHLLAIQGGISLDL
                     SQMNQVLAVNAEDLTATVQAGVTRKQLNEEIRDTGLFFPIDPGADASLGGMSATRASG
                     TNAVRYGTMRENVVSLTVVTADGRVVRTAGRARKSSAGYDLTRLFVGSEGTLGIITEV
                     TVRLYPQPEAVSAAVCNFPTLDAAVKSVIEIIQMGVPIARVEFMDAASVRAVNLHSKL
                     SLRETPMLVFEFHGSPDGVQEQARTVQAIAAENGGHNFEWAERPEDRSRLWTARHNAF
                     FAALQLRPGCRASTTDVCVPISRLADCVRETAEDLERASFPYIILGHVGDGNFHVVMM
                     LDPDSTQEWQESEAINHRLVHRAIAADGTCTGEHGIGLHKMQFMAEEHGEDALALMRS
                     LKHAFDPNNILNPGKIIAW"
     gene            1612..3111
                     /locus_tag="VGC69_10475"
     CDS             1612..3111
                     /locus_tag="VGC69_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0295745.1"
                     /translation="MNTVVDSVLVPGQEPFSAAPVIEALRRILPAHCVLYREEDTRPY
                     ECDGLPLYRCLPAAVVLPESEEQVCEVLKLCKRMNVPLVPRGAGTGLSGGALPHERGV
                     LIGLSKLNRIKRLDREACVAVVEPGVRNLAISEAAAPYGLYYAPDPSSQIACSIGGNV
                     AENSGGVHCLKYGLTVHNVLRVRMVTIEGEVVELGSEAPDAAGVDLLSVFVGSEGMLG
                     IVTEVTVKLVPKPACAQVVMASFPSVEAAGNAVTQVIASGIIPAGLEMMDRPSVRMVE
                     PFVQAGYDLEAQAILLCESDGTPEEVAHEIGRVEAVFRQAGATRCQVSVDEAERLRFW
                     AGRKNAFPAAGRVSPDYYCMDGTIPRRHLARVLTAIEQMEDEFGLRCAIVFHAGDGNL
                     HPLILFDSNKPDEVERADKFGAAILELCVQVGGTVTGEHGVGMEKINQMCVQFTRDEL
                     DTFMAVKRAFDPPMLLNPDKVIPTLARCAEYGKMHVHDGELLFPDLSRF"
     gene            3121..4218
                     /gene="glcE"
                     /locus_tag="VGC69_10480"
     CDS             3121..4218
                     /gene="glcE"
                     /locus_tag="VGC69_10480"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY0295746.1"
                     /translation="MEFVLSELTDQVLTARAAEQSLVILGGGTKSFYGNHRARRPQDG
                     HYQLDMTAYRGIVNYHPSELVVTARAGTPLAELEDELARNGQMLAFEPPHFGATATVG
                     GCVAAGLAGPRRMAAGGVRDFVLGTRVLDAQGSLVSFGGEVMKNVAGYDVSRLMAGSL
                     GIFGVLLEVSLKVLPRPLEETTRVLDTTQAEALRHFTAWRGQPLPISAAAWVPDGSGQ
                     GRLAVRLSGAPPAVQSAVARIGGRPLDDAQAWWRGLREQTHPFFDPTRPLWRVALPPT
                     APVLDWGPMLLEWGGGQRWFSGPADAQTLRAAAAHAGGHVTLFRAAGQAAPTGGVFHP
                     LAPGVATITRRLKQELDPSGLFNPGRLYPEL"
     gene            4219..5451
                     /gene="glcF"
                     /locus_tag="VGC69_10485"
     CDS             4219..5451
                     /gene="glcF"
                     /locus_tag="VGC69_10485"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY0295747.1"
                     /translation="MQTHLASWAQDTELGKEADAILRRCVHCGFCSATCPTYQVLGDE
                     LDSPRGRIYLIKQVLEGAEPTAATQAHLDRCLTCRNCETTCPSGVQYGHLVDIGRKMV
                     DERVSRPWGERLQRFALRKGLNSPLFAPAMRLGQALRGLLPPGLRRKVPLRRDIGLLP
                     DAARHARQVLMLAGCVQPAMMPTIDAATVRVLDAIGIGARVASGGGCCGAVNFHLDAQ
                     DDALAQMRANVDAWWPLVERGGIEAIVMNASGCGAMVKEYAHHLRGDPAYAARAAHIV
                     SLVKDVAEIVAPHSSQLRGRLRAEDARTVFHPPCTLQHWQGLRPLSEKVLADLGFALQ
                     PFGESHLCCGSAGAYSVLNPDMSLSLRDRKLAQVESAQAELIVSSNMGCIGHLQSGTP
                     VPVRHWIEAVDERLMASH"
     gene            5629..6942
                     /locus_tag="VGC69_10490"
     CDS             5629..6942
                     /locus_tag="VGC69_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0295748.1"
                     /translation="MTSYTAESLGALVQDGGARINGRIYTDPEIFELEMDKIFSKVWV
                     YVGHESQVPNPGDYITGLLARQPIILTRGKNGKTHVLFNRCTHRGAKIVNQDAGNEKV
                     LACMYHGWAFEMDGSLRGIPMKKDFPAAAVEDGKASLGKPPRVESYRGFIFASLNADV
                     EPLLDFLGAAASGIDEIVDRAPAGTIRLSGGYHRYVFPGNWKLQMENLADMYHPVACH
                     ISTVGADGKQFKRRPGEEGGRAAFLGPDGEPLVIQTGVRGFSHGHSSEASLFGSNDMS
                     QEAKQRGGVFDEYRSLLVRLHGAEKTDDVLKNRRHSMTLFPTLDILMVQTSVRVVIPL
                     AYDRTEVRIYPVHLDGAPDELNRQIIKFINITHSAASFIQTDDLEAFKRQQEGMAAQS
                     PEWMNVSRGQGGEIDEGRGTFFGPRASEVGQRMRHQYWRELMVAE"
     gene            6947..7456
                     /locus_tag="VGC69_10495"
     CDS             6947..7456
                     /locus_tag="VGC69_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY0295749.1"
                     /translation="MLKAISDDAVLLDDARMAPLRRFVEWECRLLDDQRFEKWLELYA
                     PTATYWAPAKHGQASPYDHVSLFWDDKETMQIRVERLLHPEIHSQIPASATMRMVNNF
                     RAVAAGEDFQVECRFLMVEDRLGSARLAFAGTLHYLLAAQGKNLQILHKRVDLTNCDH
                     AFNALTQPF"
     gene            complement(7460..8443)
                     /locus_tag="VGC69_10500"
     CDS             complement(7460..8443)
                     /locus_tag="VGC69_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295750.1"
                     /translation="MSFPSLTALRTFEAAARNHSFKLAAAELHVTPTAVSHQIQQLED
                     LLGVKLFERTGRGLLLTDAAASCLPLLQQGFDSLRQGVEKLRKHRGPDIITVNTSPSF
                     ASLWLFPRLHRFTMLYPEIDVRVTTRLRQAAKLREEQQSGVNNVQDWIQEADLIIAYG
                     NGQFGGLETEELLPLYLTPLCAPGLLPRMDKDMMASRLVKLPRLHDDRGTMYGQTSFW
                     RIWTDAMGIAPHDNVQEIHFTHAMLALSAAADGLGVVVTTPVLAESFLGDGRLHMPFD
                     HQVKIDKGYYIITETAMSASSRLAVFVEWLRQESAPYKPATAGQELPALAR"
     gene            8729..9751
                     /locus_tag="VGC69_10505"
     CDS             8729..9751
                     /locus_tag="VGC69_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295751.1"
                     /translation="MFMHRCGSVCDRSSIRRVAAAIAPPLGGAAIAMLAAMAPAQAAY
                     PDHSITLVVPYAPGGGVDGVARVFARALGEKLGQSVVVENHAGAGAMIGIDYVNRARP
                     DGYTLLVVDPAFVINPSLQPHVPYKVEDFKPISIMTGSPLVLVSAKDSKFKSFDAMLK
                     MAKSDGVSVTFATPGIGTTPHMAGELLREIQGAHMMPVAYKGSGPAATDVLSGQVDVG
                     FMSITAASSFLKQGNLQGLATTGEKRASVLPDLPTIAELTKSDYSVLFWVGCFAPAKV
                     PEEVRKKLDETIQAIATDPTYAKGLASLGETSVGSTSEVASQYVASEYGKWSALISTA
                     HIKGAE"
     gene            9805..10800
                     /locus_tag="VGC69_10510"
     CDS             9805..10800
                     /locus_tag="VGC69_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929200.1"
                     /note="similar to the 3-(2,3-dihydroxyphenyl)propionate
                     dioxygenase from E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEY0295752.1"
                     /translation="MAKVVAGIGMSHAPGALGWPDAPAASVRERLRRAAGQLGRRLEA
                     ARPDVIVAFLDDHFENHFRALMPTVALGVAQSHVGPAAQWLQALRMTEQRRFPGAPEL
                     AERLLGALVEAGFDVARMGEIEYGNNLIVPWELMAPAIAPAIIPVFINVFSPPVMPCR
                     RAYAFGDAVRQAVESLDDGLRVAFLATGGMSHWPPFWNDASPEADTFLARMKAFQTHG
                     KPVLDKDPHLLRDLAAYEIEMARRNQWPLHSPHPLVNEAWDRNMLAALAQGDVQYLCG
                     LRLEDVKREGGHGGQEILNWIALMGAMRGAPAALIDYEAVVEWICGMAYLDYGAM"
     gene            complement(10839..11588)
                     /locus_tag="VGC69_10515"
     CDS             complement(10839..11588)
                     /locus_tag="VGC69_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcI/YcgG family protein"
                     /protein_id="HEY0295753.1"
                     /translation="MDHPLSPTRCDWTCRLLHGRRLGADPGEVPGWLAQAYADYRARL
                     DDPRYPCFFGQAAERQAEMFYTSGSDAPESVGAAMSRFVELGRQTAYARFSLAMFFAP
                     DPAMDSHEAFLRRFWGALQRLNAADAGPMRPDDPDDPLWEFSFDGRKMFVVGTSPTYR
                     LRRSRKLGDGMLLLFQPRELFVDPVTGQAIPAEVRRRIHARMLDYDGMTVHPDIGFYG
                     DPGNREWKQYVLPDDNTPTTGACPFRHGGKA"
     gene            complement(11606..12478)
                     /locus_tag="VGC69_10520"
     CDS             complement(11606..12478)
                     /locus_tag="VGC69_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0295754.1"
                     /translation="MIASALSHWPPQARLWLLFAACGVLQVGLSYLICTPLEKRLPLL
                     HWPVRQPMVSDITYTFIVRIVLFPLVAYFEYDALLALVHGALAARGIAAPALVGGMAR
                     WAGTPLAGFLAGFVILDLSDYWRHRLSHRYGWWYGLHTLHHAELQMSFWSDDRSHLLE
                     DVITYIWLMVVALAIGMPALQFPFVILGFRLVGSLAHANTRARYGWLGERLLVSPQFH
                     RVHHSPDIPRGHLSNYGTVFPWWDMLFGTANFTHAARRTGDPGADLILATGNWWQQHA
                     AGVRRMRQLSRRSA"
     gene            complement(12635..13744)
                     /locus_tag="VGC69_10525"
     CDS             complement(12635..13744)
                     /locus_tag="VGC69_10525"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0295755.1"
                     /translation="MTSILDLARPEARSLPAYNSGLSSEAVRAKYGVSRVARLGSNEN
                     PFGPSPQVALALADLAAGSADYPDAGSTALREAVGRMTGIAPDCIVVGNGSEHIIEML
                     CQALLAPGDRVVTLIPSFGLHDIYPRMMGAKVDLLPVDAAMQYDVDAWVRALGQGVKL
                     VFLSNPSNPVGCMLDAAQFRRIVDAMPADGVLVVDEAYYEYALHSPGFPDTLAELKNR
                     RIHWIVLRTFSKAWGLAGLRVGYGLASGPAFVDLLNRVRTPFNVNLAAQKAALAALGD
                     PAHMRAAVLRTLAARDAMAATLRGMGLFVAPSAGNFLFFDVKRPNGPVAEGLLARGVI
                     IKPWKEPGYETYIRVSIGSDADNALFLDALQAVLG"
     gene            complement(13752..15161)
                     /locus_tag="VGC69_10530"
     CDS             complement(13752..15161)
                     /locus_tag="VGC69_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0295756.1"
                     /translation="MSQVFLDALRAVLGDKGLRTGDDIPPSHYTDWSGHTPTRPLALL
                     LPASTEEVSQALRVCHEHRIPITPQGGLTGMAGGAIPAADTVALSLSRMNVIESVDAA
                     GATLQAQAGVTLQAAQEAAHQVGMLFGVDLGARGSCQIGGNISTNAGGMEVLQYGMMR
                     EQVLGLEVVLADGTVLSMLRPMLKNNTGYDLKHWFIGSEGTLGIVTRALLRLRPAPRS
                     RATALLGLPDYAASLAVLGRLQRRFPGALTSFELMWADFYETSLAWMQARSAMAGKHP
                     FYALIAVVGNDAAALADEVQEALGEAMEAGELADAVVAQSESQAAALWRVREAPAEFP
                     TRMDPINFDVSLPLPRIGEVAQACVDEMRRRWPDMKHPMLRFGHIGDSNLHFTVNARD
                     IPVADRDEAEHLVDDLVYGLVAKAGGSISAEHGIGTLKRPYLSASRTPAELAAMRALK
                     EALDPLNLLNPGKILTPLA"
     gene            complement(15158..15976)
                     /gene="hutG"
                     /locus_tag="VGC69_10535"
     CDS             complement(15158..15976)
                     /gene="hutG"
                     /locus_tag="VGC69_10535"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEY0295757.1"
                     /translation="MSITDRPAFHFHQGTAPLLVSMPHAGTYVPPAIAARLTDDARHV
                     PDTDWHMDRLYDFARELGASILVATHSRYVIDLNRPPDGASLYPGQATTGLCPDLTFD
                     ATPVYRAKEDEPDAAEIAQRREAVWLPYHQKLAAEIARIRAEHGVAGLWDAHSIRSVL
                     PQFFEGKLPDLNFGTGKGTSCDDAMTQAITAVAKEAAGYTWVLNGRYTGGYITRHFGR
                     PQDNVHAIQLEMCQSAYMQEALPFDYLPELAAKIQPHLKRMLEAMLRFLGSKAA"
     gene            complement(16011..17411)
                     /locus_tag="VGC69_10540"
     CDS             complement(16011..17411)
                     /locus_tag="VGC69_10540"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY0295758.1"
                     /translation="MTPTHHLHAAHALLPEGWARDVLLSWNAAGDLTGVAAGVPRPAN
                     VASAAGPVLPGMPNLHSHAFQRAFGGLTEYRGQYRDKTQDSFWSWRTLMYRFAAQITP
                     ELLETLATWLYVEMLEAGYTSVCEFHYVHHDQDGRPYANRAELSLALLRAARTAGIGL
                     TLLPVLYQSGGFGGQPPSAGQRRFLNGTDDMLAMLAGLAPACAAQGARLGLAPHSLRA
                     VPPDALARAVAGLHAMDAGAPVHIHIAEQTAEVDACLAWSGQRPVQWLLEHMPVDARW
                     CLVHATHMTPGEYRDAARSGAVAGLCPTTEANLGDGIFDLPAWQADGGAWGVGSDSHA
                     SVNAAEELMMLEYSQRLALRQRNVAASPQHPEVATSMMLAAVHGGAQASGRKIAGLTA
                     GQRADFVVLDAGHVALQGLPAPSMLSGHVFASHRTSAIVRVVCGGVERVADGRHALHG
                     QAAAAFTAARARVMQS"
     gene            complement(17408..18628)
                     /gene="hutI"
                     /locus_tag="VGC69_10545"
     CDS             complement(17408..18628)
                     /gene="hutI"
                     /locus_tag="VGC69_10545"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY0295759.1"
                     /translation="MNFSTLPSADGVWHDLRLVQPDGSALSNTSLVVRDGKLAWMGPC
                     ASLPGEHAKLPRHDGRGCLATPGLVDCHTHLVYGGLRANEFAMRLAGADYEAIARAGG
                     GIVATVRATREASEDELYASTVPRLEALLADGVCAIEIKSGYGLALEHERKQLRVARR
                     LGRDYGVTVRTTFLGAHALPPEYAGRSGDYIARVCHDMLPALAEEGLVDAVDVFCERI
                     AFSLAETEQVFRAAQALGIPVKLHAEQLSDMDGAALAARYGALSCDHIEHLSQKGVDA
                     MQQAGTVAVLLPGAYYTLRDTHLPPIAALREAGVPMAVATDHNPGTSPALSLLLMVNM
                     ACTLFRLTVPEALAGVTTHAARALGIAGTHGALHLGQPANFVLWQLDEAAELAYWLGQ
                     RPIHAIVRQGRIAS"
     gene            complement(18655..19263)
                     /locus_tag="VGC69_10550"
     CDS             complement(18655..19263)
                     /locus_tag="VGC69_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEY0295760.1"
                     /translation="MSLQRFDRASLPASPWKNGGGVTREIACYPAGAGMDDFLWRLSI
                     ATVAASGPFSAFPGIDRIITLLDGPGVRLTSDDGALDHTLARPLEPFAFAGETAIHGA
                     VLGAASSDFNVMARRAKLRAQLDVLRASAACEAAEHGMLLAVSGGWHAHDAQRGAEHR
                     LEPGQGLWWAAQALAWRLAPLSPGASLIAVRLLPAARPCAAP"
     gene            complement(19260..19928)
                     /locus_tag="VGC69_10555"
     CDS             complement(19260..19928)
                     /locus_tag="VGC69_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0295761.1"
                     /translation="MHLDFSPVAAGWLDLLHGTIITVAVTAGALVLGCVLGLLVGLGR
                     LNPARRAVYVLCTAYVAVIRGTPLLVQLFIWFFGLPFFGIVLPAFVCGVIGMGIYSAA
                     YVSEIVRGAIQSVSGGQLQAALSIGMSPGVAMRYVILPQALVRMVPPLGNEFIALIKN
                     SALVSLLTIQDLMHEGQKIISVSYRSLEVYLAIAAVYFVLTGATALALHRIELRLRAG
                     GLVR"
     gene            complement(19928..>20344)
                     /locus_tag="VGC69_10560"
     CDS             complement(19928..>20344)
                     /locus_tag="VGC69_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295762.1"
                     /translation="YYAGGLVAMVKADNDSIKSIDDLNGKKVTVQVGTKSVGFLKDNY
                     PKIETVEVEKNDQMFNLVEIGRADGAVTGLPAAYQYVRVHGGLKVLPKQLTTEAYGMA
                     VSKNEPELTAAPNKSLAALKADGTYDQIVKTWFSNK"
BASE COUNT         3181 a         6507 c         7414 g         3242 t
ORIGIN      
        1 tgcgcgcgcg ccgccgggcg gtgggcatcg cggcgggtaa atagcgcggc gcgcggccgc
       61 tgaaacggac gggcgtaaaa tccaaggcag ctacttcata agatctgtac ggagacactc
      121 atgaatgccc cccagcatcc cgagactttg cgccgtcccg tcccgcaagc ctgcctggac
      181 gccttgcgcg cccgcttcgg cgagcgcttt tcggtggccg aggccgtgcg cgagcaccat
      241 ggcaaggacg agtcgccata cctgcccgtg ccgcccgacg ccgtcgtctt cgcgcacagc
      301 accgacgagg tcgcggccgt ggcccggctg tgcaacgagc atcgcgtgcc gctgatcccc
      361 tacggcacgg gctcctcgct cgaaggacac ctgctggcca tccagggcgg catcagcctg
      421 gacctgtcgc agatgaacca ggtgctggcc gtcaacgccg aggacctcac cgccacggtg
      481 caggccggcg tgacgcgcaa gcagctcaac gaagaaatcc gcgacaccgg cctgttcttt
      541 ccgatcgacc cgggcgccga tgccagcctg ggcggcatgt cggccacgcg cgcctcgggc
      601 accaatgccg tgcgctacgg caccatgcgc gagaacgtgg tgtcgctcac cgtggtcacg
      661 gccgacggcc gggtggtgcg cacggcgggc cgcgcccgca agtcctcggc cggctatgac
      721 ctgacccgcc tcttcgtggg cagcgaaggc acgctgggca tcatcacgga agtcaccgtg
      781 cggctgtatc cgcagcccga ggccgtgtcg gccgcggtgt gcaactttcc cacgctggac
      841 gcggcggtca agagcgtgat cgagatcatc cagatgggcg tgccgatcgc gcgcgtggag
      901 ttcatggacg ccgcctcggt gcgcgcggtc aacctgcaca gcaagctctc cctgcgcgag
      961 acgcccatgc tggtgttcga gttccacggc agccccgatg gcgtgcagga gcaggcgcgg
     1021 acggtgcagg ccatcgccgc ggaaaacggc ggccataact tcgagtgggc cgaacgcccc
     1081 gaagaccgca gccgcctctg gacggcgcgc cacaatgcct ttttcgccgc cctgcagctg
     1141 cgtcccggct gccgcgccag cacgacggac gtgtgcgtgc ccatctcccg cctggccgac
     1201 tgcgtgcgcg agaccgccga ggacctggag cgcgccagct ttccctacat tatcctgggt
     1261 catgtgggcg acggcaactt ccacgtggtg atgatgctcg accccgacag cacccaggaa
     1321 tggcaggagt ccgaagccat caaccatcgc ctggtgcacc gcgcgatcgc ggccgacggc
     1381 acctgcacgg gcgagcacgg catcggcctg cacaagatgc agttcatggc cgaggagcac
     1441 ggcgaggatg cgctggcgct catgcgcagc ctcaagcacg ccttcgatcc caacaatatc
     1501 ctcaatcccg gcaagatcat cgcctggtga taaacgagcg cgggtctcgc cggacgtgct
     1561 gcgggtttgt ccctgtgggc gtccggtgac cacccgctta ttgtttttgt catgaatacc
     1621 gtcgtcgaca gcgtcctggt tccaggacag gaaccgttct cagcagcacc cgtgatcgag
     1681 gcgctgcgcc gcatcctgcc cgcgcactgc gtgttgtatc gggaagagga cacccggccc
     1741 tacgaatgcg acggcctgcc gctgtatcgc tgcctgccgg cggcggtcgt gctgcccgag
     1801 agcgaggagc aggtgtgcga agtgctcaag ctgtgcaagc gcatgaacgt gccgctggtg
     1861 ccgcgcggcg cgggcacggg gctgtccggc ggggccttgc cgcatgagcg cggcgtgctg
     1921 atcggcctgt ccaagctcaa ccgcatcaag cggctcgacc gggaggcctg cgtggccgtg
     1981 gtcgagccgg gcgtgcgcaa cctggccatc tccgaggcgg ccgcgcctta cggcctgtac
     2041 tacgcgccgg atccttccag ccagatcgcc tgttccatcg gtggcaacgt ggccgagaat
     2101 tccggcggcg tgcactgcct caagtacggg ctgaccgtac acaatgtgct gcgcgtgcgc
     2161 atggtcacca tcgagggcga ggtggtggag ctgggctccg aggcgccgga tgcggccggt
     2221 gtcgatctgc tttcggtttt tgtcggttcc gagggcatgc tgggcatcgt caccgaggtg
     2281 accgtcaagc tggtgcccaa gccggcctgc gcgcaggtgg tgatggcgag ctttcccagc
     2341 gtggaggcgg ccggcaacgc ggtcacgcag gtgatcgctt ccggcatcat tcccgccggc
     2401 ctggaaatga tggaccgccc gtcggtgcgc atggtcgagc ctttcgtgca ggccggctac
     2461 gacctcgagg cgcaggccat cctgctgtgc gagtccgacg gcacgccgga agaggtcgcc
     2521 cacgaaatcg gccgggtcga ggccgtgttc cgccaggcgg gcgccacgcg ttgccaggtg
     2581 tcggtcgacg aggccgagcg cctgcgtttc tgggcgggcc gcaagaatgc ctttccggcc
     2641 gcgggccggg tgtcgcccga ctactactgc atggacggca ccattccgcg ccgccacctg
     2701 gcccgcgtgc tgaccgccat cgagcagatg gaagacgagt tcggcctgcg ctgcgccatt
     2761 gtgttccatg cgggcgacgg caatctgcac ccgttgatcc tgttcgattc gaacaagccc
     2821 gacgaggtcg agcgggccga caagttcggc gccgccatcc tcgagctgtg cgtgcaagtg
     2881 ggaggcaccg tgacgggaga gcacggtgtg ggcatggaga agataaatca gatgtgtgtt
     2941 caatttacgc gcgatgagct cgataccttc atggcggtca agcgggcttt cgacccgccc
     3001 atgctgctca accccgataa ggtcataccg acgcttgcgc gctgcgccga atacggcaag
     3061 atgcacgtgc acgatggcga actgcttttc cccgatctca gccgcttctg agggggcggc
     3121 atggaattcg tcttgtcgga attgaccgat caggtcctga cggcgcgcgc cgccgagcag
     3181 tcgctggtca tcctgggcgg cggcaccaag tcgttctatg gcaaccaccg cgccaggcgg
     3241 ccgcaggacg gccattacca gctcgatatg acggcctacc gcggcatcgt caattaccat
     3301 ccgtccgaac tcgtggtgac ggcgcgcgcc ggcacgccgc tggccgagct cgaggacgag
     3361 ttggcccgca acggccagat gctggctttc gagccgccgc atttcggcgc cacggccacg
     3421 gtgggcggct gcgtggccgc cggcctggcc gggccgcgcc gcatggcggc gggcggcgtg
     3481 cgcgacttcg tcctgggcac gcgcgtgctg gacgcgcagg gcagcctcgt gtcgttcggc
     3541 ggcgaggtca tgaagaacgt ggccggctac gacgtgtcgc ggctgatggc cggctcgctg
     3601 ggcattttcg gcgtgctgct ggaggtgtcg ctcaaggtgc tgccgcgccc gctcgaagaa
     3661 accacgcgcg tgctcgacac gacccaggcc gaggccttgc ggcacttcac ggcctggcgc
     3721 ggccagccct tgccgatctc ggcggccgcc tgggtgccgg acgggtcggg ccaggggcgc
     3781 ctggccgtgc gcctgtcggg cgcgccgccc gcggtgcagt cggccgtggc gcgcatcggc
     3841 ggcaggcccc tggacgatgc ccaggcctgg tggcgcggcc tgcgcgaaca gacgcatccc
     3901 tttttcgatc ccacgcgtcc cttgtggcgg gtggccttgc cgccgacggc gccggtgctc
     3961 gactggggcc ccatgctgct ggagtggggc ggcgggcagc gctggttcag cggcccggcc
     4021 gacgcgcaga ccctgcgcgc ggcggcggcg catgccggcg gccacgtgac cttgtttcgc
     4081 gccgccgggc aggccgcgcc cacgggcggc gtcttccatc cgctggcgcc gggcgtggcc
     4141 acgatcacgc ggcggctcaa gcaggaactc gacccttcag gcctgttcaa tccgggccgg
     4201 ctgtatccgg agttgtaaat gcagacccac ctggcttcct gggcgcaaga caccgaactg
     4261 ggcaaggagg ccgatgccat cctgcgccgt tgcgtgcact gcggcttctg ttcggccacc
     4321 tgtcccacct atcaggtgct gggcgacgag ctggacagcc cgcgcgggcg catctacctg
     4381 atcaagcagg tgctcgaggg cgccgagccc acggccgcca cgcaggcgca cctggatcgc
     4441 tgcctgacct gccgcaattg cgaaaccacc tgcccgtcgg gcgtgcaata cgggcacctg
     4501 gtcgacatcg gccgcaagat ggtcgacgag cgggtgtccc ggccctgggg cgagcgcctg
     4561 cagcgcttcg cgctgcgcaa gggactgaat tcgccgttgt tcgcgccggc catgcggctg
     4621 gggcaggcgc tgcgcggcct gctgccgccc ggcctgaggc gcaaggtgcc cctgcggcgc
     4681 gacatcgggc tgctgcccga tgccgcgcgc cacgcgcgcc aggtgctgat gctggcgggc
     4741 tgcgtgcagc cggccatgat gcccacgatc gacgcggcca ccgtccgggt gctggatgcc
     4801 atcggcatcg gcgcgcgcgt ggcctcgggc gggggctgtt gcggcgcggt caatttccac
     4861 ctggacgcgc aggacgatgc gctggcgcag atgcgcgcca atgtggacgc atggtggccg
     4921 ctggtcgagc gcggcggtat cgaggccatt gtgatgaacg cgtcgggctg cggggcgatg
     4981 gtgaaggagt acgcgcatca tctgcgcggc gatccggcct atgcggcgcg cgcggcgcat
     5041 atcgtgtcgc tggtcaagga cgtggcggag atcgtggcgc cgcattcctc gcagttgagg
     5101 ggccgcctgc gggcggagga cgcgcggacg gtgtttcatc cgccttgcac cctgcagcat
     5161 tggcaggggc tgcggccctt gagcgagaag gtgctggccg acctggggtt cgccttgcag
     5221 ccgtttggcg agagccattt gtgctgcggg tcggcggggg cgtattcggt gctcaatccg
     5281 gacatgtccc tgtccttgcg ggatcgcaag ctggcccagg tggagtcggc gcaggccgag
     5341 ttgatcgtct cgtccaacat ggggtgcatc gggcatctgc agagcggcac gccggtgccg
     5401 gtcaggcatt ggatcgaggc ggtggacgag cggctgatgg ccagccactg acggccatcg
     5461 gggcgccgga acccggatct tcctgtcgcc tgcctagtga attttcctca ggcgacgggt
     5521 gaaatatcct cgggtgacat gccccgaccg cattactagt attcgttacc accaacgacg
     5581 ggcgccggca cacggccgga gccgtcgctc ttttccatgg ggtaatgaat gacgagctat
     5641 acggcagagt cgctgggcgc gctggtccag gacggcggtg cgcgcatcaa cggccgcatc
     5701 tacacggatc ccgagatctt cgagctggag atggacaaga tcttctccaa ggtctgggtc
     5761 tacgtggggc atgaaagcca ggttccgaac ccgggcgact acatcaccgg cttgctggcg
     5821 cgccagccca tcatcctgac gcgcggcaag aacggcaaga cgcacgtcct gttcaatcgc
     5881 tgcacgcacc gcggcgcgaa gatcgtcaac caggacgccg gcaacgagaa ggtgctggcg
     5941 tgcatgtacc acggctgggc gttcgagatg gacgggtcgc tgcgcggcat tcccatgaaa
     6001 aaggatttcc cggcggcggc ggtcgaggac ggcaaggcca gcctgggcaa gccgccgcgc
     6061 gtggagtcgt atcgcggctt cattttcgcc agcctcaacg cggacgtgga gccgctgctc
     6121 gacttcctgg gggccgccgc ctcgggcatc gacgagatcg tggaccgcgc gccggcgggc
     6181 accatccgcc tgagcggcgg ctaccaccgg tatgtcttcc cgggcaactg gaagctccag
     6241 atggagaacc tggccgacat gtaccatccg gtggcctgcc atatctccac ggtgggtgcg
     6301 gacggcaagc agttcaagcg ccgtcccggc gaggagggcg ggcgcgccgc ttttctgggg
     6361 ccggacggcg agccgctggt gatccagacc ggcgtgcggg gcttttcgca cgggcacagt
     6421 tcggaggcca gcttgttcgg cagcaacgac atgtcccagg aagcgaagca gcgcggcggc
     6481 gtgttcgacg aataccgttc cctgctggtg cggctgcatg gcgccgaaaa gaccgacgac
     6541 gtcctgaaga accgccgcca cagcatgacg ctgttcccca ccctggacat cctgatggtc
     6601 cagacttccg tgcgcgtggt catcccgctg gcctacgacc gcaccgaggt gcggatctat
     6661 cccgtgcacc tggacggcgc gccggacgag ctgaatcgcc agatcatcaa gttcatcaac
     6721 atcacgcact cggccgcgtc gttcatccag accgacgacc tggaagcctt caagcgccag
     6781 caggaaggca tggcggcgca gtcgcccgag tggatgaatg tctcgcgcgg gcagggcggc
     6841 gagatcgacg aaggccgggg cacgttcttc ggcccgcggg ccagcgaagt ggggcaacgc
     6901 atgcgccatc agtattggcg cgaactgatg gtggcggagt aaacctatgc tgaaggcgat
     6961 aagtgacgat gccgtcctgc tggacgatgc ccggatggcg ccgctgcgcc gctttgtcga
     7021 gtgggagtgc cgcctgctgg acgaccagcg cttcgagaaa tggctcgagc tgtatgcccc
     7081 gacggctacg tactgggcgc cggccaagca tggccaggcc agtccctacg accatgtgtc
     7141 gcttttctgg gacgacaagg agacgatgca gatccgcgtg gagcggctcc tgcatccgga
     7201 aatacacagc cagatcccgg cctcggcgac gatgcggatg gtgaacaact tccgcgcagt
     7261 cgccgcgggc gaggatttcc aggtggagtg ccggttcctg atggtcgagg accggctggg
     7321 cagcgcaagg ctggcgttcg ccgggacctt gcactacctg ctggccgccc agggcaagaa
     7381 cctgcagatc ctgcacaagc gggtcgacct gaccaactgc gaccacgcct tcaatgcctt
     7441 gacccagccg ttctaggctt cagcgggcca gggcgggcag ctcctggccc gccgtggcgg
     7501 gcttgtaggg cgcgctttcc tggcgcagcc attccacgaa caccgccagc cggctgctgg
     7561 ccgacatggc ggtttccgtg atgatgtagt agcccttgtc tatcttgacc tggtgatcga
     7621 agggcatgtg cagcctgccg tcccccagga agctttcggc caggacgggc gtcgtgacca
     7681 cgacgccgag tccgtccgcg gcggcggaca aggccagcat ggcatgggtg aagtgtattt
     7741 cctggacatt gtcgtgcggc gcgatgccca tggcatccgt ccagatccgc cagaaagagg
     7801 tctggccgta catcgtgccg cggtcgtcgt gcagccgcgg cagcttcacc agccggcttg
     7861 ccatcatgtc cttgtccatg cgcggcagca ggccgggggc gcagaggggg gtgaggtaca
     7921 ggggcagcag ttcttccgtc tccaggccgc cgaactgccc gttgccgtag gcgatgatga
     7981 ggtccgcttc ctgtatccag tcctgcacgt tgttcacgcc gctttgctgt tcttcccgca
     8041 gcttcgcggc ctgcctgagc cgggtcgtca cccggacgtc gatttccggg tagagcatgg
     8101 tgaaccggtg caggcgcggg aacagccaca gggacgcgaa ggacggcgag gtgttgaccg
     8161 tgatgatgtc ggggccccga tgcttgcgca gtttttcgac tccttgcctg agcgagtcga
     8221 acccctgttg cagcaggggc aggcacgacg ccgccgcatc cgtcaggagc aggccgcgtc
     8281 ccgtccgttc gaacagcttc acgcccagca ggtcttcgag ctgctggatc tgatggctta
     8341 ccgccgtcgg ggtgacgtgc aattccgccg cggccagctt gaagctgtgg ttgcgggccg
     8401 cggcctcgaa tgtgcgcaag gcggtgagcg atggaaacga catgggggaa gatgagtttt
     8461 attgagttca gggatgacga atggtcgttt gaacatgcct ggccgcctct ctatgatcat
     8521 gcggcctgat cggacaggat ttgctgccat gcgttgacat gattgtgcgg cggccggttt
     8581 cacgaatcaa gcaatacggc ttcgacggca ggcggcctcg ccgggtagcg cgagcatttg
     8641 cttgcgggtt tacccgacat gcgcgcctgc gtttcgaaag acggatcgat agttcataca
     8701 tgcctgataa aaggcaaagg ggaattcaat gttcatgcat cgctgcggtt ccgtctgtga
     8761 tcgttcttcc attcgccgcg tcgccgcggc gatcgcgccg ccattggggg gcgcggcgat
     8821 cgccatgctt gcagcgatgg cgccggcgca ggcggcctat ccggatcact cgatcacgct
     8881 cgtcgtgccc tatgcgcccg gcggcggcgt cgatggcgtg gcgcgcgttt tcgcccgggc
     8941 gttgggcgaa aaactgggac agtcggtcgt cgtggagaac catgcgggcg ccggggcgat
     9001 gatcggcatc gattacgtga accgggcccg gccggacggc tacacgctgc tggtcgtcga
     9061 tccggcgttc gtgatcaatc cgagcctgca gccccatgtc ccgtacaagg tcgaggactt
     9121 caagcccatc tccatcatga ccggatcccc gctggtcctg gtatcggcca aggattcgaa
     9181 attcaagtcg ttcgatgcga tgctgaagat ggccaagtcg gacggggtca gcgtgacctt
     9241 tgcgacgccc ggcatcggga cgacgccgca catggcgggc gaattgctgc gcgagatcca
     9301 gggcgcgcac atgatgccgg tggcgtacaa gggcagcggc ccggcggcga ccgacgtgct
     9361 gtcgggccag gtggatgtgg ggttcatgag catcacggcg gcctcgtcct ttctcaagca
     9421 gggcaacttg cagggcttgg cgacgaccgg tgaaaagcgc gcgtccgtcc tgcccgacct
     9481 gccgactatt gccgaactga cgaaatcgga ctacagcgtg ctgttctggg tgggctgctt
     9541 tgccccggcc aaagtccccg aggaggtgcg caagaagctg gacgaaacca tacaagccat
     9601 cgcgacggat ccgacctacg ccaaagggct cgccagcctg ggcgagacat ccgtggggtc
     9661 cacgtcggag gtcgcgagcc aatacgtcgc gagcgaatac ggcaaatggt cggcgctgat
     9721 cagcacggcg cacatcaagg gcgcggaatg atcgggaccg gatccgggcg cgttcgttgt
     9781 caatcggcct gagcgggagt gatcatggcg aaagtggttg ccggtatcgg catgtcccat
     9841 gcgccgggcg cactgggctg gccggatgcg cccgcggcat ccgtgcgcga gcgcttgcgc
     9901 cgcgccgccg gccagctagg caggcggctg gaggccgcgc ggcccgatgt catcgtcgcg
     9961 ttcctggacg atcatttcga gaatcatttc cgcgcgctca tgcccaccgt ggcgttgggg
    10021 gtggcgcaaa gccatgtcgg ccccgcggcg cagtggctgc aagcgctgcg gatgacggaa
    10081 cagcggcgct ttcccggcgc gccggaactg gccgagcggc tgctcggcgc gctggtcgag
    10141 gcggggttcg atgtcgcgcg catgggcgag atcgaatacg gcaacaacct gatcgttccc
    10201 tgggaattga tggcgccggc catcgcgccg gctatcatcc cggtcttcat caatgtcttt
    10261 tcgccgccgg tgatgccgtg ccggcgcgcg tatgccttcg gagacgccgt gcgccaagcg
    10321 gtggagtccc tggacgacgg actgcgcgtg gccttcctgg ccaccggcgg aatgtcgcac
    10381 tggccgccat tctggaacga cgccagcccc gaggccgata cctttctggc ccgcatgaaa
    10441 gctttccaga cgcacggcaa accggtgctg gacaaggatc ctcatctgct gcgggacctg
    10501 gcggcctacg agatcgagat ggcgcggcgc aatcagtggc cgctgcactc gccccatccc
    10561 ttggtcaacg aagcgtggga ccgcaacatg ctcgcggcgc ttgcgcaagg cgatgtgcaa
    10621 tacctgtgcg gcctgcgatt ggaggacgtc aagcgcgagg gcggccacgg cgggcaggaa
    10681 atcctcaact ggatcgcgct gatgggcgcc atgcgcgggg cgcccgccgc cttgatcgac
    10741 tacgaggccg tcgtcgaatg gatttgcgga atggcctatc tggactatgg cgcgatgtag
    10801 cccggcgcca cggcggcgct gccgggcgca aggcgcggct aggccttgcc gccgtggcgg
    10861 aaggggcagg cgcccgtcgt cggcgtgttg tcgtccggca ggacgtattg cttccattcg
    10921 cggttgccgg ggtcgccgta gaagccgatg tccggatgta cggtcatgcc gtcgtagtcg
    10981 agcatgcgcg cgtggatgcg ccggcggacc tccgcgggga tggcctgccc ggtgacggga
    11041 tcgacgaaca gctcgcgagg ctggaacagc agcagcatgc cgtcgcccag cttgcggctg
    11101 cgccgcagcc ggtaggtcgg gctggtgccg acgacgaaca tcttgcgccc gtcgaaggag
    11161 aactcccaca gcgggtcgtc ggggtcgtcc ggccgcatcg ggccggcgtc ggcggcgttc
    11221 aggcgctgca gcgcgcccca gaagcgccgc aggaaggcct catggctgtc catcgccggg
    11281 tcgggcgcga agaacatcgc caggctgaag cgcgcatagg ccgtctggcg gcccagctcg
    11341 acgaagcgcg acatggccgc gcccaccgat tcgggcgcgt cgctgcccga ggtgtagaac
    11401 atttcggcct gccgttcggc ggcctggccg aagaagcagg gatagcgcgg gtcgtcgagc
    11461 cgggcgcgat agtcggcata ggcctgcgcc agccagccgg gcacctcgcc cgggtccgcg
    11521 cccagccgcc tgccatgcag cagccggcag gtccagtcgc atctcgtggg ggaaagggga
    11581 tgatccatga tgaccgcgcg cccgatcagg cgctccttct ggacagttgc cgcatgcgac
    11641 gcacgccggc ggcgtgttgc tgccaccagt tgcccgtcgc caggatgagg tcggcgcccg
    11701 ggtcgccggt gcggcgcgcc gcatgcgtga agttcgcggt gccgaaaagc atgtcccacc
    11761 aggggaagac ggtgccgtag ttgctgagat ggcccctggg gatgtcgggg ctgtggtgca
    11821 cgcggtggaa ctgcggcgag accagcagcc gttcgcccag ccagccgtag cgcgcgcggg
    11881 tattggcgtg cgccaggctg ccgaccaggc gaaagcccag gatcacgaag ggaaactgca
    11941 gggccggcat gccgatggcc agggccacta ccatcagcca gatataggtg atgacgtctt
    12001 cgagcaggtg cgagcggtcg tcggaccaga agctcatctg cagttcggca tggtgcagcg
    12061 tgtgcaggcc gtaccaccag ccgtagcggt gcgacagacg gtggcgccag tagtcgctca
    12121 agtccaggat gacgaagccg gccagaaagc ccgccagggg cgtgccggcc cagcgcgcca
    12181 tgccgccgac gagcgcgggc gcggcgatgc cgcgcgcggc cagcgcgccg tggacgagcg
    12241 cgagcagggc gtcatactcg aaatacgcga cgagcgggaa caggacgatg cggacgatga
    12301 aggtgtaggt gatgtccgac accatcggtt gccgcacggg ccagtgcagg agcggcaggc
    12361 gcttttcgag cggcgtgcag atcaggtagc tcaaacccac ctgcaggacg ccgcaggcgg
    12421 cgaacagcag ccaaaggcgc gcctggggtg gccagtgaga cagggcggat gcaatcatgg
    12481 cgggcctcct cgtgggagta tgcctgatcc atcttaacaa gccgcgcctg acagcggcac
    12541 gacaatgaaa cacgctgcaa aggcagcgtg cttcattcgg gcgggggcgc cacccagggg
    12601 gacatacccg gggagacgga gggatcgggg gggatcagcc cagcaccgcc tgcaatgcat
    12661 ccaggaacag cgcgttgtcc gcatcgctgc cgatgctcac gcggatgtac gtctcgtagc
    12721 cgggttcttt ccagggtttg atgatgacgc cgcgcgccag cagcccctcg gccacggggc
    12781 cgttggggcg cttgacgtcg aagaacagga agttgccggc cgagggggcc acgaacaggc
    12841 ccatgccgcg cagcgtggcg gccatggcgt cgcgggcggc cagggtgcgt agcacggcgg
    12901 cccgcatgtg ggccggatcg cccagggcgg cgagcgcggc cttctgcgcg gccaggttca
    12961 cgttgaaggg tgtgcgcacg cggttgagca ggtcgacgaa ggccgggccg ctggccaggc
    13021 cgtagcccac gcgcaggccg gccaggcccc aggccttgga gaaggtgcgc agcacgatcc
    13081 agtggatgcg gcggttcttc agctcggcca gcgtgtccgg aaagcccggg ctgtgcagcg
    13141 cgtattcgta ataggcctcg tccaccacca gcacgccgtc ggccggcatg gcgtcgacga
    13201 tgcggcgaaa ttgcgcggcg tcgagcatgc agcccaccgg gttggacggg ttgctcagga
    13261 acaccagctt cacgccctgg cccagggccc ggacccaggc gtcgacgtcg tactgcatgg
    13321 cggcgtcgac cgggagcaga tcgaccttgg cgcccatcat gcgcggatag atgtcgtgca
    13381 ggccgaagga cgggatcagc gtgaccacgc ggtcgccggg cgcgagcagg gcctggcaga
    13441 gcatctcgat gatgtgctcg gagccgttgc ccaccacgat gcagtccggc gcgatgccgg
    13501 tcatcctgcc cacggcttcg cgcagggcgg tcgagccggc gtcggggtag tcggcgctgc
    13561 ccgcggccag gtcggccagg gccagggcga cctggggact ggggccgaag gggttttcgt
    13621 tgctgcccag gcgcgccacg cggctcacgc cgtatttggc gcgcacggcc tcggaagaca
    13681 ggcccgagtt gtaggcgggc aggctgcggg cttcggggcg ggcgagatcg aggatggaag
    13741 tcatggcggg ttcaggcaag gggggtgagg atcttgccgg ggttgagcag gttgagcggg
    13801 tcgagcgcct ctttgagcgc ccgcatggcg gccagttcgg cgggcgtgcg gctggcggac
    13861 aggtagggcc gcttgagggt gccgatgccg tgttcggccg agatgctgcc gccggccttg
    13921 gccaccaggc cgtagacgag atcgtccacc aggtgctcgg cctcgtcgcg gtcggccacc
    13981 gggatgtcgc gcgcattgac ggtgaagtgc aggttgctat cgccgatatg gccgaagcgc
    14041 agcatggggt gcttcatgtc gggccagcgc ctgcgcattt catcgacgca ggcctgggcc
    14101 acttcgccga tcctgggcag gggcaggctg acgtcgaaat tgatcggatc catgcgggtg
    14161 gggaattcgg ccggcgcctc ccgcacgcgc cacagggcgg cggcctggga ctcggactgc
    14221 gccaccacgg cgtcggccag ctcgccggcc tccatcgcct cgcccagggc ttcctgcact
    14281 tcgtcggcca gcgcggcggc gtcgttgccg acgaccgcga tcagggcgta gaagggatgc
    14341 ttgccggcca tggccgagcg ggcctgcatc cacgcgagcg aggtctcgta gaagtcggcc
    14401 cacatcagct cgaacgaggt cagcgcgccg ggaaagcgcc gctgcaggcg gccgagcacg
    14461 gccagcgagg ccgcgtagtc gggcaggccc aggagcgcgg tggcgcggct gcgcggcgcc
    14521 gggcgcaggc gcagcagggc gcgggtgacg atgcccagcg tgccctcgga accgatgaac
    14581 caatgcttga ggtcgtagcc ggtgttgttc ttgagcatgg ggcgcagcat ggacagcacg
    14641 gtgccgtcgg ccagcaccac ctccaggccc agcacctgct cgcgcatcat gccgtactgc
    14701 agcacttcca tgccgccggc attggtgctg atgttgccgc cgatctggca ggagccgcgc
    14761 gcgcccaggt ccacgccgaa cagcatgccg acctggtgcg cggcttcctg cgcggcctgc
    14821 agggtgacgc cggcctgggc ctgcagcgtg gcgcccgcgg cgtcgacgga ttcgatgacg
    14881 ttcatgcggg acaaggacag ggctaccgta tcggccgccg ggatggcgcc gccggccatg
    14941 ccggtcaggc cgccctgcgg cgtgatggga atgcggtgtt cgtggcagac gcgcagggcc
    15001 tgcgagactt cttcggtgct ggccggcagc agcagggcca gcggacgggt gggcgtgtgg
    15061 ccgctccagt cggtgtagtg cgacggcggg atgtcgtcgc cggtgcgcaa gcccttgtcg
    15121 cccaatacgg cgcgcagggc atcgagaaag acctggctca tgcggctttg ctcccgagga
    15181 atcgcagcat ggcttcgagc atgcgcttca gatgcggctg gatcttggcg gctaactcgg
    15241 gcaggtagtc gaagggcagc gcttcctgca tataggcgct ttggcacatt tcgagctgga
    15301 tggcgtggac gttgtcctgc ggccggccga aatggcgggt gatgtagccg ccggtgtagc
    15361 ggccgttgag cacccaggtg tagcccgcgg cttccttggc gacggcggtg atggcctgcg
    15421 tcatggcgtc gtcgcagctg gtgcctttgc cggtgccgaa gttcaggtcg ggcagcttgc
    15481 cttcgaagaa ctgcggcagc accgagcgga tggagtgcgc atcccacagg ccggccacgc
    15541 cgtgctcggc ccggatgcgg gcgatctcgg ctgccagttt ctgatggtag ggaagccaca
    15601 cggcctcgcg gcgctgggcg atctcggcgg cgtcgggttc gtcttccttg gcgcggtaga
    15661 ccggcgtggc gtcgaaggtc aggtccgggc acaggccggt ggtggcctgg ccggggtaga
    15721 ggctggcgcc gtcgggcggc cggttcaggt cgatgacgta gcgcgaatgc gtggccacca
    15781 ggatggaggc gcccagctcg cgggcgaagt cgtagagcct gtccatgtgc cagtcggtgt
    15841 cgggcacgtg gcgcgcgtcg tcggtgaggc gcgcggcgat ggccggcggc acgtaggtgc
    15901 cggcatgcgg catggagacc agcaggggcg cggtgccctg gtggaaatgg aaggcggggc
    15961 ggtcggtgat ggacatgatg gacagtgtgc gaaaaacgga tgtgggtgag ctatgactgc
    16021 atgacccgcg cgcgcgcggc ggtgaacgcg gcggcggcct ggccgtgcag ggcgtggcgg
    16081 ccgtccgcca cgcgttcgac gccgccgcag acgacgcgga cgatggccga agtgcggtgg
    16141 ctggcgaaca catggccgga caacatgctg ggcgcgggca ggccctgcag ggcgacgtgc
    16201 ccggcgtcga gcacgacgaa gtcggcgcgc tggccggcag tcagtcctgc gatcttacgc
    16261 ccggatgcct gggcgccgcc atggacggcg gccagcatca tggacgtggc cacctcgggg
    16321 tgctgcgggc ttgccgcgac attgcgctgg cgcagcgcca ggcgctggct gtattcgagc
    16381 atcatcagtt cttccgcggc attgacgctg gcatggctgt ccgagcccac gccccaggcg
    16441 ccgccgtcgg cctgccaggc gggcaggtcg aagatgccgt cgcccaggtt ggcctcggtg
    16501 gtggggcaca ggccggccac ggcgccgctg cgggcggcat cgcggtattc gccgggcgtc
    16561 atgtgggtgg cgtgcaccag gcaccagcgg gcgtccaccg gcatgtgctc cagcagccat
    16621 tgcaccgggc gctggccgct ccaggccagg caggcgtcga cttcggcggt ctgctcggcg
    16681 atgtggatgt gcacgggcgc gccggcatcc atggcgtgca ggccggccac ggcccgggcc
    16741 agggcgtcgg gcggcacggc gcgcagcgaa tgcggggcca ggcccaggcg cgcgccttgc
    16801 gccgcgcagg cgggcgccag gccggccagc atggccagca tgtcgtcggt gccgttgaga
    16861 aagcggcgct ggcctgcgct gggcggctgt ccgccgaagc cgccggactg gtagagcacg
    16921 ggcagcaggg tcaggccgat gccggccgtc ctggcggcgc gcagcagcgc gagcgagagc
    16981 tcggcgcggt tggcgtaggg ccggccgtcc tggtcgtggt gcacgtaatg gaattcgcag
    17041 accgaggtgt agccggcctc gagcatttcc acatacagcc aagtggcgag cgtttccagc
    17101 agttcgggcg tgatctgcgc ggcgaagcgg tacatcagcg tgcgccagct ccagaagctg
    17161 tcctgggttt tgtcacgata ttggccgcgg tattcggtga gcccgccgaa ggcgcgctgg
    17221 aaggcgtgcg aatgcaggtt gggcatgccg ggcagcaccg ggccggcggc cgacgccacg
    17281 ttggccgggc ggggcacgcc ggctgccacg ccggtgaggt cgcccgcggc gttccagctg
    17341 agcagcacgt cgcgggccca gccttcgggc agcagggcgt gcgcggcatg caggtgatgg
    17401 gttggcgtca tgatgcgatg cgtccttggc ggacgatggc gtggatcggg cgctggccga
    17461 gccaataggc cagttcggcg gcttcatcca gttgccagag cacgaaattg gccggctggc
    17521 ccaggtgcag cgcgccgtgc gtgccggcga tgccgagcgc gcgcgcggcg tgtgtggtga
    17581 cgccggccag cgcttcgggc acggtcaggc gaaacagcgt gcaggccata ttgaccatga
    17641 gcagcaggct gagcgcgggc gaggtgccgg ggttgtggtc ggtggccacg gccatgggca
    17701 cgccggcttc gcgcagcgcc gcgatgggcg gcaggtgcgt atcgcgcagg gtgtagtagg
    17761 cgccgggcag cagcacggcc accgtgccgg cctgctgcat ggcgtctacg cctttttgcg
    17821 acaggtgctc gatgtggtcg caggacaggg cgccgtagcg cgccgccagg gcggcgccgt
    17881 ccatgtcgga caactgctcg gcatgcagct tgacgggaat gcccagtgcc tgggccgcgc
    17941 gaaagacctg ctcggtctcg gccagcgaaa aggcgatgcg ttcgcagaac acgtccacgg
    18001 cgtcgaccag gccttcttcg gccagcgcgg gcagcatgtc atggcagacc cgggcgatgt
    18061 agtcgccgct gcggccggcg tattcgggcg gcagggcatg ggcgcccagg aaggtggtgc
    18121 gcaccgtcac gccgtagtcc cggccgagcc ggcgcgccac gcgcagctgc ttgcgctcgt
    18181 gctcgagcgc gaggccgtag ccggacttga tctcgatggc gcagacgcca tcggcaagca
    18241 gggcctccag gcgcggcacg gtgctggcgt agagctcgtc ttcgctggcc tcgcgggtgg
    18301 cgcgcacggt ggcgacgatg ccgccgcctg cgcgggcgat ggcttcgtag tcggcgccgg
    18361 ccaggcgcat ggcgaactca ttggcgcgca gtccgccata gaccaggtgc gtatggcagt
    18421 cgaccaggcc gggcgtggcc aggcagcccc ggccgtcgtg gcgcggcagc ttggcgtgct
    18481 cgccgggcag gctggcgcag gggcccatcc aggccagctt gccgtcgcgc acgacgaggc
    18541 tggtgttcga aagcgccgag ccgtccggct ggaccaggcg caggtcgtgc cagacgccgt
    18601 cggcgctggg cagggtggaa aagttcatgg cgcgagggtt catggtgcgt aaggtcatgg
    18661 cgcagcgcat ggccgcgcgg cgggcagcag gcggacggcg atcaggctgg cgccgggcga
    18721 gaggggcgcg agccgccagg ccagcgcttg cgccgcccac cagaggccct ggccgggctc
    18781 gagccgatgt tccgcgccgc gctgggcatc atgggcatgc cagccgccgg acacggccag
    18841 cagcatgccg tgttcggccg cttcgcaggc ggccgatgcg cgcaggacat ccagttgcgc
    18901 gcgcagtttg gcgcggcgcg ccatgacgtt gaagtcgctg gaggccgcgc ccagcacggc
    18961 gccgtggatg gccgtctcgc cggcaaaggc aaagggttcg agcggccgcg ccagggtatg
    19021 gtcgagggcg ccgtcgtccg aggtgagccg cacgcccggc ccgtcgagca gggtaatgat
    19081 gcggtcgatg cccgggaagg ccgagaaagg cccgctggcg gccaccgtgg caatgctcag
    19141 ccgccacagg aagtcgtcca tgcccgcgcc cgccggatag caggcgatct cgcgggtgac
    19201 gccgccgccg ttcttccagg gcgaagcggg caggctggcg cggtcgaagc gttgcaggct
    19261 catcgcacca ggcctccggc gcgcaggcgc agttcgatcc ggtgcaaggc cagggccgtg
    19321 gccccggtca gcacgaaata cacggcggcg atggccaggt agacctcgag cgaacggtag
    19381 gaaacactga tgattttctg tccctcgtgc atcaggtcct ggatggtcag gagcgacacc
    19441 agggccgagt tcttgatgag cgcgatgaat tcattgccca gcggcgggac catgcgcacc
    19501 agggcctggg gcaggatgac gtagcgcatg gccacgcccg ggctcatgcc gatcgacagg
    19561 gcggcctgca actgcccgcc cgagaccgac tggatggcgc cgcgtacgat ctccgagaca
    19621 taggccgcgg aataaatgcc catgccgatc acgccgcaga cgaaggccgg cagcacgatg
    19681 ccgaagaagg gcaggccgaa gaaccagatg aagagctgca ccagcagcgg cgtgccgcgg
    19741 atcaccgcca cgtaggcggt gcacaggacg tagaccgccc ggcgggccgg attgagccgg
    19801 cccagcccga ccagcaggcc cagtacgcag cccagcacca gcgcgccggc cgtgaccgcc
    19861 acggtgatga tcgtgccgtg cagcaggtcc agccagcccg ccgcgaccgg tgagaagtca
    19921 aggtgcatta tttattgcta aaccatgtct ttacgatctg gtcgtaggtg ccgtcggcct
    19981 tgagcgcggc cagcgacttg ttcggcgcgg cggtgagctc gggctcgttc ttgctcacgg
    20041 ccatgccgta ggcctcggtg gtgagctgct tgggcagcac cttcaggccg ccgtgcacgc
    20101 gcacgtactg gtaggccgcg ggcaggccgg tgaccgcgcc gtcggcgcgg ccgatttcca
    20161 ccagattgaa catctggtcg ttcttctcga cctcgacggt ttcgatcttg ggatagttgt
    20221 ccttgaggaa gccgaccgac ttggtgccga cctgcacggt gactttcttg ccgttgaggt
    20281 cgtcgatgga cttgatgctg tcgttgtcgg ccttgaccat ggcgaccagg ccgccggcgt
    20341 agta
//
LOCUS       DASWPO010000204        23318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_670763, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000204 DASWPO010000000
VERSION     DASWPO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 23318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23318
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670763"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VGC69_10565"
     CDS             <1..620
                     /locus_tag="VGC69_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0295763.1"
                     /translation="IITRLYSEEAPFDYDGEFYHLKQAMTRPASLQRPRPVTMNAAFG
                     GPGRDFAAKHCDYLFTSFSELESGAQHVADIRARGAAAGRELGAYTVGHVVCRPTEQE
                     ARAYYDRYTIEHVDRAALDNHVKKKQGFSNSHDDKAYLLYRQRFAAGTGSYPLVGTPQ
                     QIVDELVKIQSLGFSGAALSFVNYADELPYFCSQVLPLMREAGLR"
     gene            647..1885
                     /locus_tag="VGC69_10570"
     CDS             647..1885
                     /locus_tag="VGC69_10570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0295764.1"
                     /translation="MPRSLYSKKGFFILTRRYYAGGDPAQALSIEELRRMARRRLPAF
                     VFEYLEGGAEDEVTLRRNREVFDQLAFVPRTLQRVCVVDTSVQLLGGRSALPLAIAPT
                     GFNGLMARDGDAALARAAAAAGVPFIQSTVSNATLETVSKVAGLRHWMQLYVFRSREF
                     MERLVGRALEAGCEALVLTSDGSVFGNREWDKRSYRGDMRLTWRKKLEVLGHPHWMAQ
                     VLARGIPTFGNLAELLPPEQRRLAAAAKWSREEIDADLDWDRLTWLRALWPRKLLLKG
                     VLDVEDAGRALQAGVDGVVLTNHGGRQLDGAIAPLTALPAIAGRYRDQMDIFVDSGYR
                     RGSDVVKALALGAHGVLLGRATLYGLGAAGEAGAARALAIMAGEIRRAMALLGRPAIG
                     QLDRSCLLGEPVLPASARGG"
     gene            complement(1914..2843)
                     /locus_tag="VGC69_10575"
     CDS             complement(1914..2843)
                     /locus_tag="VGC69_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295765.1"
                     /translation="MRSLDHVDLNLLRVFQAIIEEHSLTRAGARLALSQPAVSYALGR
                     LRTLFDDPLFIRTREGMQPTAVALELSAIVARAMDALREALRYAERFDPAASRRTFRL
                     SLSDAGEIAYLPGLCRLLAQQAPHVKLSIQPWPVGDMADALRTSKLDLVIGNLPGLVP
                     HTRHQVLFEEDYVCVTRRRPGLPAGRKLSLAQFQSASHIRVNSPENSHYALDETLRAR
                     GVARHIALELPHFVVLPKVLAVTDLFVILPRRLAHVLDDGGLFQLYALPFPLPAAQVT
                     MHWHEQFEAEDGLAWLRSLVEGLVDEFDLSSRR"
     gene            complement(2840..3142)
                     /locus_tag="VGC69_10580"
     CDS             complement(2840..3142)
                     /locus_tag="VGC69_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0295766.1"
                     /translation="MSLTVFRCARCGLVLFPARYFCPACGGGDWTGQAARRGRVTAAT
                     VVRRRAGAQEDVHIASVATDAGPTVVARLPRALPVGAEVALDMDAENRIEARPSTT"
     gene            complement(3139..4305)
                     /locus_tag="VGC69_10585"
     CDS             complement(3139..4305)
                     /locus_tag="VGC69_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0295767.1"
                     /translation="MASPPCAHALLAAGVEVPYSRQAPPGTTGDLLAQAFGAALAQSG
                     FTARDVDGLGVSSFTLGPDHAIDLAWRLGITPRWCMDDALGGAGALNLLQHAIRAIQG
                     GDAGVIALLAGDRFEAADFKRLVEHYNLTTQTYLRPIESGAPNSVFAMLTQRHAARYG
                     LSRTDYGALCVAQRAWAQRNPLAVYRQPLTIAEYLVAPLVADPLGRFDCVPVVCGANA
                     VIVARADRVKTARNVAVRSLQCLYNTNHQMADGLQTPLAQVAGELWRTAGAGPQDMDL
                     AAVYDDYPVMVLAQMADLGFAPRDDLRALIARIASGDLPVNTSGGQLSAGQAGAAGGM
                     HGLVEAMTQLRGQAGDRQVESARLALVTGYGMVQYRYGMCANAAVLEAVSGDGS"
     gene            complement(4341..5936)
                     /locus_tag="VGC69_10590"
     CDS             complement(4341..5936)
                     /locus_tag="VGC69_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005324 - long-chain fatty acid
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent acyl-CoA ligase"
                     /protein_id="HEY0295768.1"
                     /translation="MPGGSAASRTRLTLPALLAARVGRGADSPLFSDRATAWTAAQAM
                     DAAARWAGALAAHGIARGDRVALLCSNRKEFMEIVLGCAWLGAVVVPVNIASRGMQLA
                     HILDNSAARLAVAEAGLAESIHALEHRPQALEQVWLIGGEPAQGGPAPVDTIALPPPG
                     EALPAADVRDGDPMAILYTSGTSGPSKGVVCPHAQFYWWARYTADKLGIVPGDVLHTC
                     LPLFHTNALNTFFQALVHDAGMVADRRFSASNFFASLIDTGATVTYLLGAMVPILLGR
                     HESDAERRHRVRIALGPGVPARSVLDFKARTGIGLIDGFGSTETNFVIGGDIGQQRPG
                     YMGKLSPGFDARVVDEDDRPVPDGAAGELILRSDEPYAFALGYFGMPEKTVEAWRNLW
                     FHTGDRVVREADGYYRFIDRLKDAIRRRGENISSYEVEQVIASHPAVETVAVYAVQSS
                     LAEDEVMAAVVLRQGARLDALDLIRYCEPRMPYFAVPRYVAFAQDLPRTENGKIQKYK
                     LRAAGITATAWDLECSGYRLKRP"
     gene            complement(6060..6884)
                     /locus_tag="VGC69_10595"
     CDS             complement(6060..6884)
                     /locus_tag="VGC69_10595"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY0295769.1"
                     /translation="MNDKLTPRTVFIVSDSTGITAETFSHSVMAQFDEIDFQAIRMPF
                     IDNLDKADEVVRRIDRTALETGLKPIVFSTLVDADIQSRVRQANGLFLDLFGTFVGQI
                     EQSLGLKSSHAIGRSLMPVDSLKYQARIDAINFSLSHDDGQFINQLDKADVILVGVSR
                     CGKTPTSLYLAMQYAVMAANFPLTPDDFERGTLPKTVAPYRDKLFGLSIDPDRLSQVR
                     NERRPNSQYATLEQCRYEVAEAERMMRRESIAWMSTTTKSIEEIATTVMQEVKLGR"
     gene            7114..9483
                     /gene="ppsA"
                     /locus_tag="VGC69_10600"
     CDS             7114..9483
                     /gene="ppsA"
                     /locus_tag="VGC69_10600"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY0295770.1"
                     /translation="MSYVVSFEQLRMTDVDSVGGKNASLGEMISQLAGAGVRVPGGFA
                     TTAEAFRDFLGASGLDKRIADRLATLNPEDVRELALAGAEIRQWVIDAPFSPAFEKAI
                     RESFAKLDADGRGSFAVRSSATAEDLPDASFAGQQETFLNVVGIDDVLDKIRHVFASL
                     YNDRAISYRVHKGYEHAGVALSAGIQRMVRSDKGSAGVMFTIDTESGFQEVVFITSSY
                     GLGETVVQGAVNPDEFYVFKPALAKGHYPIVSRRIGSKLIKMEFNGEHKPGENAVRTV
                     DVPVSERNRYSLTDDEVCELARYAVIIEKHYGRPMDVEWGRDGIDGKLYILQARPETV
                     KSQQTGDVQQRYRLKATGDVLITGRAIGQKIGSGPVRIVADVSEMDKVQPGDVLVTDM
                     TDPNLEPVMKRASAIVTNRGGRTCHAAMIARELGIPAVVGTGEGTNLLKEGQPVTVSC
                     AEGDEGRIYDGLIETEVEEVRRGAMPDIDLKIMMNVGNPQLAFDFAQIPNGGVGLARL
                     EFIINNNIGIHPKAVLDYPNVDSELKKAVESAARGYASPRAFFVEKLAEGIATIGAAF
                     YPKSVIVRLSDFKSNEYRKLVGGSRYEPEEENPMLGFRGASRYISADFAECFKMECEA
                     LKKVRDVMGLTNVEIMVPFVRTVSQAERVVKLLAGHGLKRGENGLKLIMMCEVPTNAI
                     LADEFLAHFDGFSIGSNDMTQLTLGLDRDSGMELLAADFDERDEAVKFLLRRAIQACL
                     KAGKYVGICGQGPSDHPDFAQWLKDEGILSISLNPDTVVDTWQKLAATK"
     gene            9714..9923
                     /locus_tag="VGC69_10605"
     CDS             9714..9923
                     /locus_tag="VGC69_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295771.1"
                     /translation="MKDDLKFMGAEVGRLATGMDRFATKDELKLLGTELRGEINLLKW
                     MYSPVALVALVWISKEAPKVFGVTG"
     gene            complement(9975..10697)
                     /locus_tag="VGC69_10610"
     CDS             complement(9975..10697)
                     /locus_tag="VGC69_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY0295772.1"
                     /translation="MTASPLPVLILHTGDPDAALRRQHGNYGEQMRRVAGLAPADARI
                     VAVYQGETPEAPENYRAVLITGSPAMVTDHEPWSEAAAAWLREAVDAGLPAFGVCYGH
                     QLLAYALGGEVDYNPAGSGLGTLPVQRLADDPLTEGLPAEFPAQLLHAQVVTRPPAGA
                     TALAQASHDAYQLLRYAPGVYSSQFHPEFDTDFMREHLLHHAAYYGNKGYDAPAVAEK
                     LAPTRHASNLVRRFLALHAPAA"
     gene            10739..11161
                     /locus_tag="VGC69_10615"
     CDS             10739..11161
                     /locus_tag="VGC69_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY0295773.1"
                     /translation="MLTWFGLAVLALIGEVLTGTFYLLLVALGLAAGGVAAWLSYSPE
                     WQVIAFGVIGLAALVVLRKTRVLKKREIDSQRNADVNLDIGQPVEVAGWAPDGTAQVW
                     YRGATWQADLAEGETPQPGTHVIAAMRGSRLLLARKPA"
     gene            11227..12159
                     /locus_tag="VGC69_10620"
     CDS             11227..12159
                     /locus_tag="VGC69_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEY0295774.1"
                     /translation="MMFDSSLSVLVIIAVLALVIIVKSIAIVPQQHAWVIERLGRYDR
                     TLSPGAGFVIPFIERVAYRHSLKEIPLAVPSQVCITRDNTQLQVDGVLYFQVTDAMRA
                     YYGSSNYINAITQLAQTTLRSVIGKLELDRTFEERAIINSSIVAALDEAALNWGVKVL
                     RYEIKDLTPPAEILHAMQAQITAERGKRALIAASEGRRQEQINIATGEREAAIARSEG
                     EQQAQINQAQGEAAAVVAIAEATSKAIIQVAQAIQQPGGMEAVNLKVAERYVEAFGQV
                     AKQGNTLILPANLADMGGLIASAMTVVKASNRGS"
     gene            complement(12336..12803)
                     /gene="smpB"
                     /locus_tag="VGC69_10625"
     CDS             complement(12336..12803)
                     /gene="smpB"
                     /locus_tag="VGC69_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY0295775.1"
                     /translation="MSIIDNRKASHDYFIEDRYEAGIVLEGWEVKAIRAGRVQLKESY
                     VIVRDGEIYLLGMHVSPLATASTHIHPDAMRTRKLLLNAEEIHKLIGKVEQRGYTLVP
                     LNLHYKNGRVKLDFALGRGKKLFDKRDTSREKDWEREKDRIMKHDTRNSRRDD"
     gene            12869..13303
                     /locus_tag="VGC69_10630"
     CDS             12869..13303
                     /locus_tag="VGC69_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY0295776.1"
                     /translation="MHTVQRSVLVPYSAAQMFDLVADVEKYPEFMPWCGGTEVQSRDE
                     HGMQASVLISFAGLKQRFTTRNRHDYPSRIDLDLVDGPFSSLAGHWQFLSLAEDACKV
                     VFTLDYAFSSRALEAVVGPVFNRIATSFIDSFTKRAQAKYGE"
     gene            13293..13643
                     /locus_tag="VGC69_10635"
     CDS             13293..13643
                     /locus_tag="VGC69_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEY0295777.1"
                     /translation="MANEAAPGPCLRVQVCYALHDSVWQRDLALPQGATVAQALAASG
                     LAEDFPDLDVWALGAGLYGKLAAPGTPLADGDRIEIYRALSFDPKESRRRREEHRRAK
                     QAAQGRRERPPGLL"
     gene            complement(13679..14401)
                     /locus_tag="VGC69_10640"
     CDS             complement(13679..14401)
                     /locus_tag="VGC69_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0295778.1"
                     /translation="MIDSLRVSSAPSLREQVVVRLREAIALGHFAPGERLRERVLCEL
                     TGVSRTSLREALRELESEGLVTSVPNRGIIVSRIDLDLARSTFELRGVLEELAVTLFV
                     QRAGPAHGVVLKQAFEALRQAYDRDDSASILEGKTGFYDAILEGGGNPLLKPALKSIH
                     VRVSQLRSASLAQPSRRAESLREFAALFEAMQRRDLAAAQAACREHVARAAVAALEGL
                     AQSARQDPGPEQYESKAAPVLE"
     gene            14601..15572
                     /locus_tag="VGC69_10645"
     CDS             14601..15572
                     /locus_tag="VGC69_10645"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295779.1"
                     /translation="MRRFLRTFILFILGAAFSLPAMADPSYPSGPVTLVVPFGPGGGT
                     DLIARLFAKPFAQAIGGSVIVRNHGGSGTTIGTAYVAHSRPDGLTLLLNGSTLTYHPA
                     MYKSLPYDVKKDLIPIAFISNQPYALIVNKDFPAKTLGDLVALAKQSPGQIPYGSAGI
                     GSAMHLSAELLWENLGIKMLHVPYPGTGQAMNDLIAGRVKAVYTTAAGAAGMVRAGTV
                     RALGVSSPQRMATMPDVPTIAESGLPGYQHGSWMALFAPAGTPPAVVEQIAQATNKAL
                     ADPELIRQFTAQGLETKIGSPTEVKQFFDKEVDRWTGVIKAAGIEAQ"
     gene            15574..16359
                     /locus_tag="VGC69_10650"
     CDS             15574..16359
                     /locus_tag="VGC69_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0295780.1"
                     /translation="MDNYLEVNGIRIRYRVEGSGDWLVLIHGVGGRLEQWDQFVALLQ
                     GQYRTLRFDQRGMGETSKPAGPYHIDDYVNDAYALMEAVGVDRCHLAGCSLGALVAQG
                     LALAHPEKLKKLIFLAGVAGRTEEEKRRVQERLGIVAGGIPGQHFENSVIRWFTDEFR
                     AQHPDVIAAYAASNRKNDPKAYAAAYAVLANTDFAERLHEIEIPTLVVTGENDMGSNP
                     RMAQFMHEQIAGSQVAILPKLRHSLLIEGPQQVLDVVGPFLKS"
     gene            16399..16986
                     /locus_tag="VGC69_10655"
     CDS             16399..16986
                     /locus_tag="VGC69_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEY0295781.1"
                     /translation="MSFDIRKIVCFKEIIHREGGKPLAKPITMLGVAAVIRNPWHGRG
                     FVEDLSPEQHDGCAALGQVMVERILQEIGGADVVESYGKASVVGMDGEIEHASAVIHN
                     LRFGNIYRDAVSAETYLSFTNKRGGPGTSLQIPMKHLHEASARSHFITLEFSIPDAPA
                     ADEIVVLLGAATGGRAHPRIGDRNLDRVEMAKEAS"
     gene            17090..18244
                     /locus_tag="VGC69_10660"
     CDS             17090..18244
                     /locus_tag="VGC69_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0295782.1"
                     /translation="MDLRLTEEQQAFAQAARDYAEGELAPHAAQWDEEGIFPKRAFAR
                     AGELGFCGLYASEDIGGLGLPRLDAALVFEEMAAVDPSTTAFLTIHNMVAWIIGQWGG
                     AAVRQAWGALLASGEKLGSYCLTEPGAGSDAGSLATRAERDGGSYLISGAKAFISGAG
                     DTDLLVVMARTGGPGPGGISAFAVPADTPGISYGRKENKMGWNSQSTRPIAFDRVRVP
                     AANLLGQEGEGFRIAMKGLDGGRINIATCSVGAAQGALDAARRYMDERRQFNRKLSEF
                     QALQFKLADMATHLVAARQMVRLAACKLDAGDPEATTYCAMAKRFATDMGFQICLDAQ
                     QIHGGYGYLKDYPLERLVRDTRVHQILEGTNEIMRVIVARQLLEKGADIR"
     gene            18241..19428
                     /locus_tag="VGC69_10665"
     CDS             18241..19428
                     /locus_tag="VGC69_10665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0295783.1"
                     /translation="MSSTTNTPANAAPVLFEERFTDNGMRLGVATLNQPQTLNGLSLD
                     MVDLLQARLTAWAADPMVAAVILQGAGEKAFCAGGDLHSLYRSMRDYAGQGAWSNVYA
                     RTFFEREYRLDYCIHTYPKPLLCWGRGIVMGGGVGLMMGASHRVLSTDARVAMPEVGI
                     GLFPDVGGSWMLNRMPGRLGLFLALTGAQLNASDAIHTGLADHALDPDRWDALCQVLM
                     QQPWAGNPHQGGPAARSINDGLLRRAIGTFAPRQDDKPGPLRQHAFMINGLCGESQLE
                     DIHAGIAQLATHPDPWLARAAKTMLAGAPGSVRLSYMLQQNTRLRSLEDVFRMEYIVA
                     LHCCSSRGNLAEGIRALLIDKDRQPRWQPATLREADADWAWGFFTEPWPSDKVHPLAD
                     LGS"
     gene            19501..20394
                     /gene="mmsB"
                     /locus_tag="VGC69_10670"
     CDS             19501..20394
                     /gene="mmsB"
                     /locus_tag="VGC69_10670"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEY0295784.1"
                     /translation="MSRIAFIGLGNMGAPMALNLIRAAHELTVFDLVPAAAQALADAG
                     ASVAASAQAAVPGADIVITMLPASKHVEGLYLGDAGLLKHIDPRSLVIDCSTIAPDSA
                     RKVAEAARALGLAMIDAPVSGGTGGAAAGTLTFIVGGAADALARAHPVLEKMGKNIFH
                     AGPSGAGQVAKICNNMLLGILMAGTAEALALGVASGLDPRVLSDIIAKSSGRNWATEL
                     YNPWPDVMPNAPASKGYAGGFGTDLMLKDLGLAAETAMSARSATPLGELARNLFAMHS
                     QAGHGKLDFSSILKLYTREPE"
     gene            20525..21208
                     /locus_tag="VGC69_10675"
     CDS             20525..21208
                     /locus_tag="VGC69_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEY0295785.1"
                     /translation="MPAREHHRIYRVGWLRAAVLGANDGIVSTASLIAGVAAAQASHP
                     AIWVAGVAALVAGALSMAAGEYVSVHSQADTEAADLKLEMGSLKRNADQELEELTGIY
                     MARGLAPGLALQVATALTRHDALDAHARDELGISVDHRARPVQAAVASAFSFASGAAL
                     PLVIAMLAPLAHLVAIVLLGSVICLAILGAVAAHAGGAPIGRAVLRVMILGAAAIALT
                     AGIGSLFGA"
     gene            complement(21221..22228)
                     /locus_tag="VGC69_10680"
     CDS             complement(21221..22228)
                     /locus_tag="VGC69_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY0295786.1"
                     /translation="MPTPFYAGLPAHLLDAPTCAALDGFRVIAATGDEALDFLHGQLT
                     QDVTGLAADRASLAGYCTAKGRLLATLAMWRAPADPDAADARWHALVRADLAEALIKR
                     LSMFVLRAKAKLVLTPLHAAGVQCEGPQRAALQAAAGGALPAEPWQRADLPTGTWIAA
                     PAASNAAGPRWWWIASDAQLGGAQALSQVLAQGDAQAWQAADLAAGLPWIGSLTQDMF
                     IPQTVNLELAGGVSFTKGCYPGQEVVARSHYRGTVKRRMAYGQLDDAGATVRPGADIY
                     DAAQPHEPSGRVVDAAGPALLFETTLASLPDGDLRLGAADGPRIRVLPLPYALPEQDA
                     A"
     gene            22392..>23318
                     /gene="mltG"
                     /locus_tag="VGC69_10685"
     CDS             22392..>23318
                     /gene="mltG"
                     /locus_tag="VGC69_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0295787.1"
                     /translation="MKIRLRTLFLLFPLLIVALVAGVVGGFVLWANQPVQMPSAKIDF
                     IVDPGSTPRAITRALADAGIPIWAPGFVWLARLTERDKLIKSGGYEAVQGDSPWTLLE
                     RMARGERSERQLAFIEGWTFKQMREALHNAPDLKQTLDGLSDEEVMARLGADIKHPEG
                     LFFPDTYVFAAGSTDFDVLRRAYAAGQQILDEAWEKRAPNLPVATPYEALILASLVEK
                     ETSQRAERARVAGVFINRLRKGMLLQTDPSVIYGLGDAYDGHIHKRDLLADTPWNTYT
                     RPGLPPTPIALAGRAALNAALHPEANNYLYFVA"
BASE COUNT         3867 a         7986 c         7837 g         3628 t
ORIGIN      
        1 agatcatcac gcggctgtat tcggaagagg cgcccttcga ctacgacggc gagttctacc
       61 acctgaagca ggccatgacc cgcccggcgt cgctgcagcg gccccggccg gtcacgatga
      121 acgcggcctt cggcggtccc ggccgtgatt tcgccgcgaa gcattgcgac tatctgttca
      181 cgtcgttcag cgagctcgag agcggcgcgc agcacgtcgc cgacatccgc gcccgcggcg
      241 ccgcggccgg ccgcgagctg ggcgcctaca ccgtcggcca cgtggtctgc cggcccacgg
      301 agcaggaggc cagggcctat tacgaccgct acacgatcga gcacgtcgac cgtgccgcgc
      361 tcgacaatca cgtcaagaag aagcagggct tcagcaacag ccacgacgac aaagcctatc
      421 tgctgtatcg ccagcgcttc gccgccggca ccggcagcta ccccctggtg ggcacgccgc
      481 agcagatcgt cgacgagctg gtcaagatcc agagcctcgg tttctcgggc gcggccctgt
      541 cgttcgtcaa ctacgccgac gagctgccgt atttctgcag ccaggtgctg ccgctgatgc
      601 gcgaggcggg cctgcgctga tccgggagga cggcgcgcgg cgcggcatgc cgcgatccct
      661 ctacagcaag aaaggattct tcatcttgac gcgccgctat tacgcaggag gcgacccggc
      721 ccaggccttg tcgatcgagg agctgcgccg catggcgcgc cggcgcctgc cggccttcgt
      781 gttcgaatac ctggagggcg gcgccgaaga cgaggtgacg ctcaggcgca accgcgaggt
      841 gttcgatcaa ctggcgttcg tgccgcgcac gctccagcgg gtttgcgtcg tggatacgtc
      901 ggtccagctg ctgggcggcc ggtctgccct gccgctggcc atcgcgccca cgggcttcaa
      961 cggcctcatg gcgcgcgacg gcgacgcggc gctggcgcgc gccgcggcgg cggcgggcgt
     1021 gcccttcatc cagagcacgg tgtcgaacgc caccctcgag acggtgtcga aagtcgccgg
     1081 cctgcgccac tggatgcagc tgtacgtctt ccgctcgcgc gagttcatgg aacggctcgt
     1141 cggccgggcg ctcgaggccg gctgcgaggc gctggtgctg acgtccgacg gctcggtctt
     1201 cggcaaccgc gaatgggaca agcgcagcta ccgcggcgac atgcgcctga cctggcgcaa
     1261 aaagctggag gtgctggggc atccgcactg gatggcgcaa gtgctggcgc gcggcatccc
     1321 gaccttcggc aacctcgccg aactgctgcc tcccgagcag cgccgcctgg ccgcggccgc
     1381 caaatggtcg cgcgaggaga tcgacgccga tctcgactgg gacaggctga cgtggctgcg
     1441 cgccctctgg ccgcgcaagc tgctgctcaa gggcgtgctc gacgtcgaag acgcgggccg
     1501 cgccctgcag gccggcgtcg acggcgtcgt gctgaccaac catggcgggc ggcagctcga
     1561 cggcgccatc gcgcccctga ccgccttgcc ggccatcgcc gggcgctatc gcgaccagat
     1621 ggacattttc gtcgacagcg gctatcgccg cggcagcgac gtcgtcaagg cgctcgcgct
     1681 gggcgcgcat ggcgtgctgc tggggcgcgc gaccctatac gggctgggcg cggcgggcga
     1741 ggcgggggcc gccagggcgc tggccatcat ggcgggcgag atccggcggg cgatggccct
     1801 gctgggccgg ccggccatcg gccagctgga ccgctcctgc ctgctggggg agccggtcct
     1861 gccggcatcg gcccgtgggg gatagatagc ctggatgctg tcgctggagc gtttcagcgg
     1921 cggctggaga ggtcgaattc gtccaccagg ccctcgacca ggcttctcag ccatgccagg
     1981 ccgtcctcgg cctcgaattg ctcgtgccaa tgcatggtga cctgggccgc gggcaagggg
     2041 aagggcaggg catagagctg gaaaagcccg ccgtcgtcca ggacatgcgc cagccggcgc
     2101 ggcaggatca cgaacaggtc ggtcacggcc aggaccttgg gcaggaccac gaagtgcggc
     2161 agctccagcg cgatgtggcg cgcgacgcct cgcgcgcgca gcgtttcgtc gagcgcatag
     2221 tggctgttct cgggcgagtt gacacgaatg tgcgaggcgg actggaactg tgccagcgac
     2281 agcttgcggc cggcgggcag gcccggtcgg cggcgcgtga cgcagacgta gtcttcttcg
     2341 aacaggactt ggtggcgcgt gtgcggcacc aggccgggca ggttgccgat gaccaggtcg
     2401 agcttgctgg tgcgcagcgc gtcggccatg tcgccgacgg gccagggctg gatgctcaac
     2461 ttgacgtgcg gcgcctgctg cgccagcagg cggcacaggc ccggcaggta ggcgatctcg
     2521 ccggcgtcgg agagggacag cctgaaagtg cgccggctgg cggcggggtc gaagcgctcg
     2581 gcgtagcgca gcgcttcgcg cagcgcgtcc atggcacggg cgacgatggc cgacagctcc
     2641 agcgcgaccg ccgtgggctg catgccttcg cgggtgcgga tgaagagcgg gtcgtcgaac
     2701 agcgtgcgca gccgccccag cgcgtagctc acggcgggct gggacagggc caggcgcgcg
     2761 ccggcccggg tcaggctgtg ctcttcgatg atggcctgga agacgcgcag caggttcagg
     2821 tcgacgtggt ccagggatct catgtggtgc tcggtcgggc ctcgatgcgg ttttccgcgt
     2881 ccatgtccag cgctacctcg gcgccgacgg gcagcgcgcg gggcaggcgc gccaccaccg
     2941 tcggcccggc gtcggtggcg acgctggcaa tgtgcacgtc ttcctgcgcg cccgcgcggc
     3001 ggcgcacgac ggtggcggcc gtcacccggc cgcgccgcgc ggcctgcccg gtccagtcgc
     3061 cgccgccgca cgcggggcag aaatagcggg cgggaaacag gacaagcccg caccgcgcgc
     3121 agcggaacac ggtcaggctc atgatccgtc tccggacacg gcttcgagca cggcggcgtt
     3181 ggcgcacatg ccgtagcggt actgcaccat gccatagccc gtcaccaatg ccagcctggc
     3241 cgactcgacc tggcgatcgc cggcctggcc gcgcagctgg gtcatggcct cgaccaggcc
     3301 gtgcatgccg ccggcggcgc ccgcctggcc ggcgctgagc tggccgcccg aggtgttgac
     3361 cggcaggtcg cccgaggcga tcctggcgat cagcgcccgc aggtcgtccc ggggcgcgaa
     3421 acccaggtcg gccatctgcg ccagcaccat gacgggatag tcgtcgtaga cggcggccag
     3481 gtccatgtcc tgcggcccgg cgccggcggt gcgccagagc tcgccggcca cctgggcgag
     3541 cggcgtctgc aggccatcgg ccatctggtg gttggtgttg tagaggcatt gcaggctgcg
     3601 caccgcgaca ttgcgcgcgg tcttgacccg gtcggcccgg gccacgatga cggcgttggc
     3661 gccgcacacc accggcacgc agtcgaagcg ccccagcggg tcggcgacca gcggggccac
     3721 caggtactcg gcgatggtca gcggctggcg gtagaccgcc agcggattgc gctgcgccca
     3781 ggcgcgctgc gccacgcaca gcgcgccgta gtccgtccgc gacaggccgt atcgcgcggc
     3841 gtgccgctgc gtcagcatgg cgaacacgct gttgggggcg ccgctctcga tggggcgcag
     3901 gtaggtctgc gtggtgaggt tgtagtgctc gacgagccgc ttgaagtcgg cggcctcgaa
     3961 gcggtcgccc gccagcaggg cgatgacgcc ggcgtcgccg ccctggatgg cccggatggc
     4021 gtgctgcagc aagttgagcg ccccggctcc gcccagggcg tcgtccatgc accaccgcgg
     4081 cgtgatgccc aggcgccacg ccaggtcgat ggcgtggtcg ggccccagcg tgaacgagct
     4141 cacgcccagg ccgtcgacgt cgcgcgccgt gaagcccgac tgggccaggg ccgcgccgaa
     4201 ggcctgcgcc agcaagtcgc cggtggtgcc cggcggcgcc tggcggctgt agggcacttc
     4261 cacgccagcc gccagcaagg cgtgcgcgca gggggggctg gccatgagcg gctcctgccg
     4321 agaaggttgg agaggcacgg ctacggccgc ttcagccggt agcccgagca ttccaggtcc
     4381 caggccgtcg cggtgatgcc ggccgcgcgc agcttgtact tctggatctt gccattttcg
     4441 gtcctgggca ggtcctgggc aaacgccaca tagcgcggca cggcgaagta gggcatcctg
     4501 ggctcgcaat accggatgag atcgagcgcg tccaagcggg cgccctggcg cagcaccacc
     4561 gccgccatga cctcgtcctc cgccagggaa gactgcacgg catagacagc cacggtttcc
     4621 accgccggat ggctggcgat gacctgctcg acctcgtacg acgagatgtt ctccccgcgc
     4681 cgccggatcg cgtccttgag gcggtcgatg aagcggtagt agccgtcggc ttcccgcacc
     4741 acgcggtcgc ccgtgtggaa ccagaggttg cgccaggcct cgacggtctt ttccggcatg
     4801 ccgaaatagc ccagcgcgaa ggcgtagggt tcgtcggacc gcaggatgag ctcgcccgcc
     4861 gcgccgtcgg gcacggggcg gtcgtcctcg tcgaccaccc gggcgtcgaa gccgggcgac
     4921 agcttgccca tatagcccgg ccgctgctgg ccgatgtcgc cgccaatgac gaagttggtt
     4981 tcggtggagc cgaagccgtc gatcaggccg atgccggtgc gcgccttgaa gtccaggacg
     5041 gaccgggccg gcacgccggg ccccagggcg atgcgcacgc ggtgccggcg ctcggcgtcc
     5101 gactcgtgcc ggcccagcag gatgggcacc atggcgccca gcagataggt caccgtggcg
     5161 ccggtgtcga tcagggacgc gaagaaattg ctggccgaga agcgccggtc ggccaccatg
     5221 ccggcatcat gcaccagggc ctggaagaag gtgttgagcg cgttggtgtg gaacagcggc
     5281 aggcaggtgt ggagcacgtc gcccggcacg atgcccagct tgtcggcggt gtagcgcgcc
     5341 caccagtaga actgggcgtg cgggcacacc acgcccttgg acgggccgga agtcccggag
     5401 gtgtagagga tggccatggg gtcgccgtcg cggacgtcgg ccgcgggcag ggcctcgccc
     5461 ggcggcggca gggcaatcgt gtccaccggc gccgggccgc cctgggcggg ttcgccgccg
     5521 atcaaccaga cctgctcgag ggcctggggc ctgtgctcca gggcgtggat cgactcggcc
     5581 aggccagcct cggccaccgc cagccgggcc gcgctgttgt ccaggatgtg cgcgagctgc
     5641 atgccgcgcg aagcgatgtt gacggggacc acgaccgcgc cgagccaggc gcagccgagc
     5701 acgatctcca taaattcctt gcggttcgag cacagcaggg ccacccggtc gccgcgcgcg
     5761 atgccgtgcg ccgccagggc gccggcccac cgcgcggcgg cgtccatggc ctgcgcggcc
     5821 gtccaggcgg tggcgcggtc cgagaacaag ggggagtccg cgccgcgccc gacccgggcg
     5881 gcgagcaggg cgggcaaggt caatcgggtc cgggatgctg cgctgccgcc tggcatgaga
     5941 ggcttccttc atctgatgag gcagtctagg agcgtcccgg caaatcagtc aaatttattt
     6001 aatatcagtc caattatcaa taggactgat gatcctggcg tcggctcgag cactcgaaat
     6061 caccggccga gcttcacttc ctgcatcacc gtggtcgcga tttcctcgat cgacttggtc
     6121 gtcgtcgaca tccaggcgat gctttcgcgg cgcatcatcc gctcggcctc ggccacctcg
     6181 taccggcatt gctccagcgt cgcatactgg ctgttgggcc ggcgctcatt gcgcacctgc
     6241 gacaggcggt ccgggtcgat cgaaagaccg aacagcttgt cgcgataggg cgcgaccgtc
     6301 ttgggcagcg tgccgcgctc gaaatcgtcc ggcgtgagcg ggaaattggc cgccatcacc
     6361 gcgtattgca tcgccaggta caggctggtg ggcgtcttgc cgcagcgcga cacgcccacc
     6421 aggatcacgt cggccttgtc gagctggttg atgaactgcc cgtcgtcgtg cgagaggctg
     6481 aaattgatgg cgtcgatgcg ggcctggtac ttcagggaat ccaccggcat cagcgagcgc
     6541 ccgatggcat ggctggactt cagtcccagc gactgctcga tctggcccac gaaggtgccg
     6601 aacaggtcca ggaacagccc gttggcctgg cgcacccgcg actggatgtc cgcatccacc
     6661 agggtgctga agacgatggg cttgagcccg gtctccaggg ccgtccggtc gatgcgacgg
     6721 accacctcgt cggccttgtc caggttgtcg atgaagggca tgcggatcgc ctggaaatcg
     6781 atctcgtcga actgcgccat caccgaatgg ctgaaggttt cagccgtgat gcccgtgctg
     6841 tcggaaacga tgaaaacggt gcggggagtg agcttgtcat tcatgtgtaa aaatatccga
     6901 cagagcgccc aaaggctagg gttttaccag gggtgagcag gtacaatcgg ccctctgtgc
     6961 acaggcattt cgacagtcac gccaaaagca gctttgcagc aaacaaggcc ggtttggtca
     7021 gcgcggttcc cgggtgcggt tttcaacgca gttttcaagc ttcccggctc tctgaccaaa
     7081 ctcgctttct ttccattgta aaaggtgact tcaatgtcgt acgttgtttc gttcgagcag
     7141 ctccgcatga cggatgtgga ctcggtagga ggcaagaacg catcgcttgg tgaaatgatc
     7201 agccagctgg ctggcgccgg cgtgcgcgtg ccgggcggct tcgccaccac ggccgaggct
     7261 ttccgcgact ttctcggcgc ctcggggctg gacaagcgca tcgccgaccg cctggccacg
     7321 ctcaaccccg aggatgtccg cgaactggcc ctggccggcg cggaaatccg ccagtgggtc
     7381 atcgacgccc cgttctcgcc cgcgttcgaa aaagccatcc gcgagtcctt cgccaagctc
     7441 gacgccgacg gccgcggctc gttcgccgtg cgttcgtcgg ccaccgccga agacctgccc
     7501 gacgcctctt tcgccggcca gcaggaaacc ttcctcaacg tggtcggcat cgacgacgtg
     7561 ctcgacaaga tccgccacgt cttcgcctcg ctctacaacg accgcgccat ctcctaccgc
     7621 gtgcacaagg gctacgaaca cgccggcgtg gcgctgtcgg ccggcatcca gcgcatggtg
     7681 cgttccgaca agggtagcgc cggcgtgatg ttcaccatcg acaccgaatc gggcttccag
     7741 gaagtggtct tcatcacctc gtcgtacggc ctgggcgaga ccgtggtgca gggcgccgtc
     7801 aaccccgacg agttctacgt cttcaagccc gcgctggcca agggccacta tcccatcgtc
     7861 agccgccgca tcggctccaa gctgatcaag atggagttca atggcgagca caagcccggt
     7921 gaaaacgccg tgcgcacggt ggacgtgccc gtgtccgagc gcaaccgcta ctcgctcacc
     7981 gacgatgaag tctgcgaact cgcgcgctac gccgtcatca tcgagaagca ctacggccgc
     8041 cccatggacg ttgaatgggg ccgcgacggc atcgacggca agctctacat cctgcaggcc
     8101 cgccccgaga ccgtgaagtc gcagcaaacc ggcgacgtgc agcagcgcta tcgcctgaag
     8161 gccaccggcg acgtcctcat caccggccgc gccatcggcc agaagatcgg ttccggcccc
     8221 gtgcgcatcg tggccgacgt gtccgaaatg gacaaggtcc agcccggcga cgtgctggtc
     8281 accgacatga ccgaccccaa cttggagccc gtcatgaagc gcgcctcggc catcgtcacc
     8341 aaccgcggcg ggcgcacctg ccacgcggcc atgatcgccc gcgagctggg catcccggcc
     8401 gtggtcggca ccggcgaagg caccaacctg ctcaaggaag gccagcccgt caccgtgtcg
     8461 tgcgccgaag gcgacgaagg ccgcatctac gacggcctga tcgagaccga ggtcgaggaa
     8521 gtgcgccgcg gcgccatgcc cgacatcgat ctcaagatca tgatgaacgt gggcaacccg
     8581 cagctggcct tcgacttcgc gcagatcccc aacggcggcg tcggcctggc ccgcctggaa
     8641 ttcatcatca acaacaacat cggcatccac cccaaggccg tgctcgacta tcccaacgtc
     8701 gacagcgagc tcaagaaggc ggtggagtcg gcggcccgcg gctatgccag cccgcgcgcc
     8761 ttcttcgtcg agaagctggc cgagggcatc gccaccatcg gcgcggcctt ctaccccaag
     8821 tccgtcatcg tgcgcctgtc ggacttcaag tccaacgagt atcgcaaact ggtgggcggt
     8881 tcgcgctacg agcccgagga agaaaacccc atgctgggct tccggggcgc ctcgcgctac
     8941 atctctgccg atttcgccga gtgcttcaag atggaatgcg aagccctcaa gaaggtgcgc
     9001 gacgtgatgg gcctgaccaa cgtcgaaatc atggtgccct tcgtgcgcac cgtgagccag
     9061 gccgagcgcg tggtcaagct gctggccggc cacggcttga agcgcggcga gaacggcctg
     9121 aagctcatca tgatgtgcga agtgccgacc aacgccatcc tggccgacga gttcctggcg
     9181 catttcgacg gcttctcgat cggctcgaac gacatgaccc agctcaccct gggcctggac
     9241 cgcgactcgg gcatggaact gctggccgcc gatttcgacg agcgcgacga ggccgtgaag
     9301 ttcctgctgc gccgtgccat ccaggcttgc ctgaaggccg gcaagtacgt gggcatttgc
     9361 ggccagggcc ccagcgacca tcccgacttc gcgcagtggc tcaaggacga gggcatcctg
     9421 tcgatttcgc tgaatccgga tacggtggtc gatacctggc agaagctggc ggcgacgaag
     9481 taagccggcg cgcatcggcc ggcggcacgt gttgccgtcg gccaggaaac aggctccccg
     9541 ttaccggggg cctgtttcgt tttcagcgcc gtgccaaggc cggatgaaat cgacggaccg
     9601 ggtcaaccga caggcgcgtt ttcatccacc acggcttgta cgaatagcgc agtcggcagg
     9661 ccttgtcgat actgacgctt ccctttgcga tggaccgacc gggttttcca tgcatgaagg
     9721 acgatctcaa gttcatgggg gctgaagtcg ggcgcctcgc cactgggatg gatcgcttcg
     9781 ccaccaagga cgagctcaag ctcctgggga ccgaattgcg cggcgaaatc aatctgctca
     9841 aatggatgta cagcccggtc gcgttggtgg cgctggtctg gatcagcaag gaagcgccca
     9901 aggtcttcgg cgtgacgggt tgattttccc gcttttgcca ggcggcaggc ccggttcccc
     9961 cgggccttta cgccttaagc agccggcgca tgcagcgcca ggaaacgccg caccaggttc
    10021 gacgcgtgcc gcgtcggggc gagcttttcg gccacggccg gcgcatcgta gcctttgttg
    10081 ccgtagtagg ccgcgtgatg cagcaggtgc tcgcgcatga agtccgtgtc gaattccgga
    10141 tggaactggc tggaatacac gccgggcgcg tagcgcagca attggtaggc gtcatggctg
    10201 gcctgggcca gcgccgtggc gccggcgggc gggcgcgtga ccacctgggc atgcagcagc
    10261 tgcgccggga actcggcggg caggccttcg gtgagcgggt cgtcggccag gcgctgcacg
    10321 ggcagggtgc ccaggccgct gccggccggg ttgtagtcca cctcgccgcc cagcgcatag
    10381 gccagcaact ggtgcccgta gcacacgccg aaggcgggca ggcccgcgtc caccgcctcg
    10441 cgcagccagg cggccgcggc ttcgctccag ggttcgtggt cggtgaccat ggcgggcgag
    10501 ccggtgatga gcacggcacg gtaattttcg ggggcttcgg gcgtttcgcc ctggtagacg
    10561 gccacgatgc gggcgtctgc gggcgccagg ccggcgacgc gccgcatctg ttcgccgtag
    10621 ttgccgtgct gccgccgcag ggcggcatcc gggtcgccgg tgtgcaggat gaggacgggc
    10681 aggggggatg cggtcataat aggtgggcgc tgtaaacctt tgcgggaagc atacaacgat
    10741 gttgacgtgg ttcggcctgg ctgtgctggc cttgatcggc gaggtgctca ccggcacctt
    10801 ctacctgctg ctggtcgcgc tgggcctggc ggccggcggc gtcgcggcgt ggctgtccta
    10861 ctcgcccgaa tggcaagtga tcgccttcgg cgtgatcggg ctggcggcgc tcgtcgtgct
    10921 gcgcaagacg cgcgtgctga aaaaacgcga aatcgattcg cagcgcaacg ccgacgtcaa
    10981 tctcgatatc ggccagccgg tcgaggtggc cggctgggcg cccgacggca cggcacaggt
    11041 ctggtaccgg ggcgccacct ggcaggccga cctggccgaa ggcgaaacgc cccagcccgg
    11101 cacccacgtc atcgcggcga tgcgcggcag ccgcctgctc ctggcccgca agcccgcctg
    11161 acggcaggac aggccggcac acgcttcgcc caccccgtct ttccccatac accccaagag
    11221 gcagtcatga tgttcgattc ttccctatcg gtcctggtca tcatcgccgt gctggcgctg
    11281 gtgatcatcg tcaagtccat cgccatcgtg ccgcagcagc atgcctgggt gatcgagcgc
    11341 ctgggcaggt acgaccgcac gctgtcgccg ggcgcgggct tcgtcattcc cttcatcgag
    11401 cgcgtggcct acaggcactc gctcaaggaa atcccgctgg ccgtgcccag ccaggtctgc
    11461 atcacgcgcg acaacacgca gctgcaggtc gacggcgtgc tgtatttcca ggtcaccgac
    11521 gcgatgcggg cctattatgg ctcgtcgaac tacatcaatg ccatcaccca gctggcgcag
    11581 accacgctgc gctcggtgat cggcaagctc gagctcgacc gcaccttcga ggagcgagcc
    11641 atcatcaaca gcagcatcgt cgcggcgctg gacgaggccg cgctcaactg gggcgtgaag
    11701 gtgctgcgct acgaaatcaa ggacctcacc ccgccggccg agatcctgca cgccatgcag
    11761 gcgcagatca ccgccgaacg cggcaagcgg gcgctgatcg ccgcctcgga aggccgccgg
    11821 caggagcaga tcaacatcgc cacgggcgag cgcgaagcgg ccattgcccg ctcggaaggc
    11881 gagcagcagg cgcagatcaa ccaggcgcag ggcgaggcgg ccgccgtggt ggccatcgcc
    11941 gaggctacct ccaaggcaat cattcaggtg gcgcaggcga tccagcagcc gggcggcatg
    12001 gaggccgtga acctgaaggt ggccgaacgc tacgtggaag ccttcgggca agtggccaag
    12061 cagggcaaca cgctgatcct gccggccaac ctggccgaca tgggcgggct gatcgcctcg
    12121 gcgatgacgg tggtgaaggc gtcgaaccgg ggcagctagc cacatgtttt tgctgccgtg
    12181 gagcgatgaa accgcgagct gcggaccccg gaggaatccg aagcgcaggc gctgccagcg
    12241 ccgagcatcg gaattccgca gggcagccgc agcggttcaa ggaccccgac gcgcctcacg
    12301 aacgccgagc caagccggcc cgcacagaat caacatcagt cgtcgcgacg gctgttgcgc
    12361 gtgtcgtgct tcatgatgcg gtccttctcg cgctcccagt ccttctcgcg cgacgtatcg
    12421 cgcttgtcga agagcttctt gccgcggccc agcgcgaaat ccagcttcac ccggccgttc
    12481 ttgtagtgca ggttgagcgg caccagcgtg tagccgcgct gctcgacctt gccgatcagc
    12541 ttgtggattt cctccgcatt gagcaagagc ttcctcgtgc gcatcgcgtc ggggtggata
    12601 tgcgtggagg cggtcgccag cgggctcacg tgcatgccca gcaggtagat ctcgccatcg
    12661 cgcacgatga cgtagctttc cttgagctgc acgcgtcccg cccggatggc cttgacctcc
    12721 cagccttcca ggacaatgcc ggcctcgtag cggtcttcga tgaagtaatc gtgcgaagcc
    12781 ttgcggttat cgataatgct cataggggaa aggatagtac agcgcaaagc cggtaaaatc
    12841 tttcaattct agccatttgc cctgatcgat gcacaccgtc cagcgttccg tcctcgttcc
    12901 ttactcggcg gcccagatgt tcgacctcgt cgccgacgtg gaaaagtacc cggaattcat
    12961 gccctggtgc ggcggcacgg aggtgcagtc gcgcgacgag cacggcatgc aggcctcggt
    13021 gctcatcagc ttcgccggac tcaagcagcg cttcaccacc cgcaaccggc acgactaccc
    13081 cagccgcatc gacctggacc tggtcgacgg cccgttctcc agcctggccg gccactggca
    13141 gttcctgtcc ctggccgagg acgcctgcaa ggtcgtcttc accctggact acgccttttc
    13201 cagccgcgcg ctggaggccg tggtggggcc ggtgttcaac cgcatcgcca ccagcttcat
    13261 cgattcgttc accaagcgcg cgcaggccaa gtatggcgaa tgaggcggcc cccggcccct
    13321 gcctgcgcgt gcaggtctgc tatgccctgc acgacagtgt ctggcagcgg gacctggcgc
    13381 tgccccaagg cgccaccgtc gcccaggccc tggcggccag cggtttggcc gaagattttc
    13441 cggacctgga tgtctgggcg cttggcgcgg gcctctacgg caagctcgcc gcgcccggca
    13501 cgccgctcgc cgacggcgac cgcatcgaaa tctatcgcgc cctgagcttc gaccccaagg
    13561 aatcccgccg ccggcgcgag gagcaccgcc gcgccaagca ggcggcgcaa gggcgccgcg
    13621 aacgcccgcc gggcctgctt taagccgtat cggccgcgcc ttgaacgcct gcccgggcct
    13681 attccagcac cggcgcggcc ttgctctcgt attgctccgg gcctgggtcc tgccgggccg
    13741 attgcgccag gccttccagg gcggccaccg ccgcacgcgc cacatgctcg cggcatgccg
    13801 cttgcgccgc ggccaggtcg cggcgctgca tggcctcgaa cagcgcggca aactcgcgca
    13861 gcgattcggc cctgcgggag ggctgggcca atgaggcgga ccgcagttgc gaaacgcgga
    13921 catggatgct cttgagggcg ggcttcagca agggattgcc cccgccttcc aggatggcgt
    13981 cgtaaaagcc cgtcttgcct tcgaggatgc tggccgaatc gtcgcggtcg taggcctgcc
    14041 gcagcgcctc gaaggcctgc ttcaggacga cgccatgggc gggcccggca cgctgcacga
    14101 acagcgtcac cgccagttct tccaggaccc cgcgtagctc gaaggtcgat cgcgccaggt
    14161 ccaggtcgat ccgggacacg atgatgcccc ggttgggcac cgacgtgacc aggccctcgc
    14221 tttccagttc tcgcagcgcc tctcgcaaag aggtgcggga aacccccgtc agctcgcaca
    14281 gcacgcgctc tctgaggcgc tcgcccgggg cgaagtgccc tagcgcaatg gcctcccgca
    14341 agcggacaac cacttgttca cgcagggaag gcgcggacga gactcgcagg gaatcgatca
    14401 aagggcaggg ctccgatggg gtccggtgac caggcattcc cgggcacgga cgacaggatc
    14461 aacgatgcgg ccggcttgct ccgcggactg ggtaagcaat catacaaatt ccagtgacgg
    14521 gttattgtca tgattattgt attaccataa tattgtcaga caaaattaaa cgctgaaata
    14581 aaaccaaggg gaaattcgcc atgcgtcgct tcttacgcac cttcatcctg ttcatcctcg
    14641 gggccgcctt ctccctgccc gccatggccg acccgtcgta tccctcgggt cccgtcaccc
    14701 tggtcgtgcc tttcgggccg ggcggcggca ccgacctgat cgcccgcctg ttcgccaagc
    14761 cgttcgcgca agcgatcggc ggctcggtca tcgtcaggaa ccacggcgga tcgggcacga
    14821 ccatcggcac ggcctacgtc gcgcattccc ggccggacgg gctgaccttg ctgctcaatg
    14881 gcagcacgct gacctatcac ccggccatgt acaagtcgct gccgtacgac gtcaagaaag
    14941 acctgatccc gatcgccttc atttcgaacc agccctacgc cttgatcgtc aacaaggact
    15001 tcccggccaa gacgctgggc gacctggtgg ccttggccaa gcagtccccc gggcagattc
    15061 cctatggcag cgccgggatc ggcagcgcca tgcacctgtc ggccgaactg ctctgggaaa
    15121 acctgggcat caagatgctg cacgttccct atcccggaac gggccaggcg atgaacgacc
    15181 tgatcgccgg ccgcgtcaag gcggtctaca ccacggccgc gggcgccgcc gggatggtcc
    15241 gcgccggcac ggtgcgggcg ctcggcgtga gcagccccca gcgcatggcc accatgcccg
    15301 acgtgccgac catcgcggaa tcgggcctgc cgggctatca gcatggcagc tggatggcct
    15361 tgttcgcgcc ggcgggaacg ccgccggccg tcgtggagca gatcgcccag gcgacgaaca
    15421 aggcgctcgc cgatcccgaa ttgatcaggc aattcacggc gcaagggctc gaaaccaaaa
    15481 tcggcagccc gaccgaagtg aagcaattct tcgacaagga agtcgatcgc tggaccggcg
    15541 tcatcaaggc ggcaggcata gaggctcaat gaaatggata actacctgga agtaaacggc
    15601 atccggatcc gctatcgggt cgagggcagc ggcgattggc tggtcttgat ccacggcgtc
    15661 ggcggacgcc tggagcaatg ggaccaattc gtggcactgc tgcaagggca gtaccgcacc
    15721 ttgcgattcg accagcgcgg catgggggag acctcgaagc ccgcggggcc ctaccacatc
    15781 gatgattacg tcaacgatgc ctatgcgctc atggaggccg tgggcgtcga ccgctgccat
    15841 ctggcgggct gttcgctggg cgccctggtg gcgcagggcc tggcgctggc ccacccggag
    15901 aagctcaaga agctgatctt cctggccggc gtcgccggac ggaccgagga agaaaaacgc
    15961 agggtgcagg aacggctcgg catcgtcgcc ggcggcatac cggggcagca tttcgagaat
    16021 tcggtgatcc ggtggttcac cgacgaattc cgcgcgcagc atcccgacgt catcgcggcc
    16081 tatgccgcca gcaatcggaa gaacgacccc aaggcgtacg cggcggccta tgccgtgctg
    16141 gccaataccg atttcgccga acgcctgcac gaaatcgaga tccccacgct cgtcgtgacg
    16201 ggcgaaaacg acatgggttc gaacccccgc atggcgcaat tcatgcacga gcagatcgcc
    16261 ggctcccaag tcgcgatcct gccgaagctg cgccactcgt tgctcatcga aggcccccag
    16321 caggtgctgg acgtcgtggg gcccttcctg aaatcctgac cccgccaccc cggcgcattt
    16381 ccacaacaag gagaggacat gtctttcgac atcagaaaaa tcgtgtgttt caaggaaatc
    16441 atccatcgcg agggcggcaa gccattggcc aagcccatca ccatgctggg cgtggcggcc
    16501 gtcatccgca acccctggca cggacgcggc ttcgtcgagg acctgagccc cgaacagcat
    16561 gacggctgcg cggccctggg ccaggtcatg gtcgagcgga tactgcagga aatcggcggc
    16621 gcggacgtgg tggaaagcta cggcaaggcc agcgtcgtcg gcatggatgg cgaaatcgag
    16681 cacgcctcgg ccgtcatcca caacctgcga ttcggcaaca tctatcgcga cgcggtgtcg
    16741 gcggagacct acctgagctt caccaacaag cggggcggac cgggaacctc tttgcaaatc
    16801 ccgatgaagc atctgcacga agcctcggcg cgttcgcatt tcatcaccct ggagttttcc
    16861 attcccgatg cgccggccgc ggacgagatc gtcgtcctgc tcggcgcggc aaccggcggg
    16921 cgcgcgcatc cccgcatcgg tgatcgaaac ctggaccggg tggagatggc gaaggaagcc
    16981 tcgtagtcat tcaaaaagcg gtagccaagg cgtaagataa gcggacaaaa atgacgttga
    17041 cgtcaacgtc aacgcagaca aagcgatccg ccgcacgagg agacaacccg tggatctacg
    17101 ccttaccgaa gagcagcagg ctttcgccca ggctgcccgc gactacgccg aaggcgaact
    17161 ggcgccgcat gccgcgcagt gggacgaaga gggcatcttt cccaagcggg ccttcgcccg
    17221 ggccggcgaa ctcggctttt gcggcctcta cgccagcgaa gacatcggcg ggctgggcct
    17281 gccgcgcctg gacgccgccc tggtcttcga ggaaatggcc gccgtcgatc cctccaccac
    17341 ggcctttctc accatccaca acatggtggc ctggatcatc ggccagtggg gcggcgcggc
    17401 cgtcaggcaa gcctggggag ccttgcttgc cagcggcgaa aaactcggct cctactgcct
    17461 caccgagccc ggcgccggat cggatgccgg ctcgctggcc acccgcgccg aacgcgacgg
    17521 cggcagctat ctcatctccg gcgccaaggc cttcatctcg ggcgccggcg acaccgatct
    17581 gctggtggtc atggcccgca ccggcggccc cgggcccggc ggcatcagcg ccttcgccgt
    17641 gccggccgac acgcccggca tcagctacgg ccgcaaggaa aacaaaatgg gctggaacag
    17701 ccagtccacc cggcccatcg ccttcgatcg ggtgcgcgtg cccgccgcca acctgctggg
    17761 ccaggaaggc gagggctttc gcatcgccat gaagggcctg gacggcggac gcatcaacat
    17821 cgcgacctgt tccgtcggcg cggcccaggg cgcgctggac gccgcgcgcc gctacatgga
    17881 cgagcgccgc cagttcaatc gcaagctgtc cgaattccag gccctgcaat tcaagctggc
    17941 cgacatggcc acgcacctgg tggccgcccg gcagatggtg cgccttgccg cctgcaagct
    18001 cgacgcgggc gaccccgagg ccaccaccta ctgcgccatg gccaagcgct tcgccaccga
    18061 catgggcttc cagatctgcc tggacgccca gcagatacac ggcggctatg gctatttgaa
    18121 agactacccg ctcgagcgcc tggtgcgcga tactcgggtg catcaaatcc tcgaaggcac
    18181 caacgaaatc atgcgcgtca tcgttgcccg ccaattgctg gaaaaaggag ccgacattcg
    18241 atgagcagca cgacgaacac cccagccaac gccgcgccgg tcctcttcga ggaacgcttc
    18301 accgacaacg gcatgcgcct gggggtggcc acgctcaacc agccgcagac gctcaacggc
    18361 ctgtcgctgg acatggtgga cctgctgcag gcgcgcctga ccgcctgggc ggccgacccg
    18421 atggtggccg cggtgatcct gcagggcgcg ggtgaaaagg ccttctgcgc gggcggcgac
    18481 ctgcacagcc tttaccgcag catgcgcgac tacgccgggc agggcgcctg gagcaatgtc
    18541 tacgcgcgca cgttcttcga acgcgaatac cgcctcgact actgcatcca cacctatccc
    18601 aagccgctgc tgtgctgggg ccgcggcatc gtcatgggcg gcggggtggg gctgatgatg
    18661 ggcgccagcc accgcgtgct cagcaccgat gcgcgcgtgg ccatgcccga ggtcggcatc
    18721 ggcctgtttc ccgacgtggg cggcagctgg atgctcaacc gcatgcccgg ccggctcggc
    18781 ctgttcctgg cgctgacggg cgcgcagctc aatgcctcgg acgccatcca taccggcctg
    18841 gccgaccatg cgctcgaccc ggaccgctgg gacgcgctct gccaggtgct gatgcagcag
    18901 ccctgggccg gcaacccgca ccagggcggc cccgcggcgc gctccatcaa cgatggcctg
    18961 ctgcgccgcg cgatcggcac tttcgctccg cggcaggacg acaagccggg tccgctgcgc
    19021 cagcacgcct tcatgatcaa cggcctgtgc ggcgaatccc agctggaaga catccatgcc
    19081 gggatcgccc agctggcaac gcaccccgac ccctggctgg cccgcgccgc caagaccatg
    19141 ctggccggtg cgcccggctc ggtgcggctg tcgtacatgc tgcagcagaa cacccgcctg
    19201 cgctcgctgg aggatgtgtt ccgcatggaa tacatcgtgg cgctgcattg ctgctcgtcg
    19261 cgcggcaacc tggccgaagg catccgcgcc ttgctcatcg acaaggacag gcagccccgc
    19321 tggcagccgg ccacgctgcg ggaggccgac gccgattggg catggggctt cttcaccgag
    19381 ccctggccgt cggacaaggt gcatccgctg gccgacctgg gcagctgaac gcgcacgaca
    19441 aacccatccg gcaacaacag ggcacaagcc cggctgccat ggttcaacga ggagacaagc
    19501 atgagccgca tcgcattcat cggcctgggc aatatgggcg cgcccatggc gctgaacctc
    19561 atccgcgccg cacacgaact cacggtgttc gacctggtgc cggccgccgc gcaggcactg
    19621 gccgacgccg gcgcctcggt cgccgcctcg gcccaggctg ccgtgccggg cgccgacatc
    19681 gtcatcacca tgctgccggc cagcaagcac gtcgaggggc tgtacctcgg cgatgccggt
    19741 ttgctgaagc acatcgaccc gcgcagcctg gtgatcgact gcagcaccat cgcgcccgat
    19801 tcggcccgca aggtggccga ggccgcccgc gcgctgggcc tggccatgat cgacgcgccg
    19861 gtgtccggcg gcacgggcgg ggccgcggcc ggcacgctca ccttcatcgt gggcggggcc
    19921 gccgacgcgc tggcgcgggc ccatccggtg ctggaaaaaa tgggcaagaa catcttccac
    19981 gccggcccct cgggcgcggg gcaggtggcc aagatctgca acaacatgct cctgggcatc
    20041 ctcatggcgg gcaccgccga ggcgctcgcc ctgggcgtgg ccagcgggct cgacccgcgg
    20101 gtgctctcgg acatcatcgc caagagctcg gggcgcaact gggccaccga gctttacaac
    20161 ccctggccgg acgtgatgcc caatgcgccg gcttccaagg gctatgccgg gggcttcggc
    20221 accgacctca tgctcaagga cctggggctg gccgccgaga ccgccatgtc ggcgcgttcg
    20281 gccacgccgc tgggcgagct ggcccgcaat ctgttcgcca tgcacagcca ggccggccac
    20341 ggcaaactgg atttctccag catcctcaag ctgtacacgc gcgaacccga ataaaaacca
    20401 ttgcggatgc ggtttacgat cgacacgggc aggccgcaaa acgcggctcc cgggctctat
    20461 actcgcggct gattttttcc agccactatt tgcaagcggg ccgcaggccc gcgccgctgc
    20521 gcccatgccc gcaagagaac atcaccgcat ataccgcgtc ggctggctgc gcgccgccgt
    20581 gctcggcgcc aacgacggca tcgtctccac cgccagcctg atcgccggcg tggccgccgc
    20641 ccaagccagc catcccgcca tctgggtggc cggcgtggcg gccctggtgg cgggcgcctt
    20701 gtcgatggcg gccggcgaat acgtgtcggt gcacagccag gccgacaccg aggccgccga
    20761 tctcaagctg gaaatgggtt cgctcaagcg caacgccgac caggagctcg aagagctgac
    20821 cggcatctac atggcgcgcg gcctggcgcc cggcctggcc ctgcaagtgg ccaccgcgct
    20881 cacccgccac gatgcgctgg acgcgcatgc gcgcgacgag ctgggcattt cggtggacca
    20941 ccgggcccgg cccgtgcagg ccgcagtcgc gtcggcgttt tctttcgcct cgggcgcggc
    21001 gctgcccctg gtgatcgcca tgctggcgcc gctggcgcac ctggtggcca tcgtgctgct
    21061 cggttcggtg atctgcctgg ccatcctggg cgcggttgcc gcccacgccg gcggggcgcc
    21121 tattgggcgg gcggtcctgc gcgtcatgat tctgggcgcc gcggccattg cgctgacggc
    21181 cggcatcggt tcgttgttcg gcgcctaggc ccgaatacgt tcaggcggcg tcttgctcgg
    21241 gcagggcgta gggcaggggc aggacgcgga tgcgcgggcc gtcggccgcg cccaggcgca
    21301 aatcgccatc gggcagcgaa gccagcgtgg tctcgaacag cagggccggt cccgcggcgt
    21361 ccaccacgcg gccgctgggt tcgtgcggct gcgcggcgtc gtagatatcg gcgccgggcc
    21421 ggacggtcgc gccggcatcg tccagttggc cgtaggccat gcggcgcttg accgtgccgc
    21481 ggtaatggct gcgcgccacc acttcctggc ccggatagca gcccttggtg aaactgaccc
    21541 ccccggccag ttccaggttg acggtctgcg gaatgaacat gtcttgcgta aggctgccga
    21601 tccagggcag gccggcggcc aggtcggccg cttgccaggc ttgcgcgtcg ccttgcgcca
    21661 atacctgcga cagggcctgg gcgccgccga gctgcgcatc cgaggcgatc caccaccagc
    21721 gcgggccagc cgcattggat gcggcaggcg cggcgatcca ggtgccggtg ggcaggtcgg
    21781 cgcgctgcca gggctcggcg ggcagggcgc cgcccgcggc ggcctgcagg gccgcccgct
    21841 gcggaccctc gcactgcacg cccgcggcat gcaggggcgt cagcaccagc ttggccttgg
    21901 cccgcagcac gaacatcgac aggcgcttga tcaaggcctc ggccaggtcg gcgcgcacca
    21961 gggcatgcca gcgggcatcg gccgcgtccg ggtcggccgg cgcgcgccac atggccagcg
    22021 tggccagcaa gcggcccttg gccgtgcaat agccggccag gctggcccgg tcggccgcca
    22081 ggccggtgac gtcctgcgtg agctggccgt gcagaaagtc cagggcctcg tcgcccgtgg
    22141 cggcgatcac gcgaaaaccg tccagcgcgg cgcaggtcgg ggcatctagc aaatgggcgg
    22201 gcagcccggc gtagaaaggt gtcggcataa aatttctgtt gaggcgtgtt acggcaaggc
    22261 cagcgcgagc ctcccaggca atgtcccatg ataccgtgcc cccttgatcc agaccggcca
    22321 tgcccgcgcc gccttcaggg gcagttctcc ccgcgtccgg cggggcatat ccattaaact
    22381 gcgcgatagc catgaagatt cgtttgcgca ctctctttct gttgtttccg ctgctcatcg
    22441 tcgcgctggt cgcgggggtg gtcggcggct tcgtgctatg ggccaaccag cccgtgcaga
    22501 tgccctcggc caagatcgat ttcatcgtcg atccgggcag cacgccgcgc gccatcaccc
    22561 gcgcgctggc cgatgccggc attcccatct gggcccccgg cttcgtctgg ctggcccggc
    22621 tcaccgaacg cgacaagctc atcaagtccg gcggctacga ggccgtgcag ggcgattcgc
    22681 cctggacgct gctcgagcgc atggcgcgcg gcgagcgctc cgagcgccag ctcgccttca
    22741 tcgagggctg gaccttcaag cagatgcgcg aagcgctgca caacgcgccc gacctcaagc
    22801 agaccctgga cggcctgagc gacgaggagg tcatggccag gctgggcgcc gacatcaagc
    22861 accccgaagg cctgttcttc cccgacacct acgtattcgc cgcgggcagc accgatttcg
    22921 acgtcctgcg ccgcgcctat gcggccggcc agcagatcct cgacgaggcc tgggaaaagc
    22981 gcgcgcccaa cctgccggtg gccacgccct acgaggccct gatcctggcc tcgctggtcg
    23041 aaaaggaaac cagccagcgc gccgagcgcg cccgtgtcgc cggcgtgttc atcaaccgcc
    23101 tgcgcaaggg catgctgctg cagaccgacc cctcggtcat ctatggcctg ggcgacgcct
    23161 acgacgggca catccacaag cgcgacctgc tcgccgacac cccctggaac acctacacgc
    23221 gcccgggcct gccgcccacg cccatcgcgc tggccggccg cgccgcgctc aacgcggccc
    23281 tgcatcccga ggccaacaac tacctgtatt tcgtggcg
//
LOCUS       DASWPO010000205         4805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_493263, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000205 DASWPO010000000
VERSION     DASWPO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493263"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VGC69_10690"
     CDS             complement(<1..136)
                     /locus_tag="VGC69_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY0295788.1"
                     /translation="MPHVIFHKNGQTYAGEVKEGANLVVRAGIKQFPFPHLAYQCGMG
                     K"
     gene            410..1138
                     /locus_tag="VGC69_10695"
     CDS             410..1138
                     /locus_tag="VGC69_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY0295789.1"
                     /translation="MSMLMSRDEFRSALENAIKGKSANQAPFSIAWAAGKLSRAHLSR
                     WAENHYHYVGPFADYLGYLYARTPDHMTEAKDFLLANMYEEEIGGDRHTDLLIRFAEA
                     CGTTRERVTNPDNMSPTTRGLQAWCYSVAMREDPVVAVAALVVGLESQVPSIYRKQTP
                     TLREKYGFSDEEVEFFDLHIVSDEIHGERGYQIVLEHADTPELQARCLKICEIGAQMR
                     LLYTTALYRDYVANDVSEEELLPA"
     gene            1234..2691
                     /locus_tag="VGC69_10700"
     CDS             1234..2691
                     /locus_tag="VGC69_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0295790.1"
                     /translation="MTDSIKHYQNYIDGQWQPCQSGRTFDNINPADTAEIVGRFQASS
                     AADAEAAVAAAAKAFPAWRGTPISKRARILTDAADYLEAHADEFARDLTREEGKALGQ
                     ARDEILRSAQTFRFYAVEGQTFTGETYPQDDPLMHVYSHREPLGVVTVISPWNFPASI
                     AARKIAPALITGNTVVFKPSSDAPLTGLHLAEALAHAGAPAGAVNCLTGRAGDVGPVI
                     TTSRAVRAISFTGSTQAGAQIHRSVHLTTRLQMELGGKNPLLVMEDADLDAAVDLAIK
                     GGFSLTGQACTGTSRIIIVPQVKEAYTRKLLERIKRLKIGPGLEAGMDLGPLATARQL
                     DTVLQYIAIGKQEATHLIGGEHLQGGRYGTGYYVAPAVFTDVKPGMRIAQEEIFGPVL
                     AIIEAADFQEALAIANDSEYGLSAAIATRNPRTMHLFAQDIESGTVKINRTTTGNLVN
                     APFGGLKNSSTSTFRESGRAGLDFFTQIKTIYRGV"
     gene            2726..3202
                     /locus_tag="VGC69_10705"
     CDS             2726..3202
                     /locus_tag="VGC69_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0295791.1"
                     /translation="MKAAQTLELHEARAMAQAALEKAREIGVTETVCITDAGGFPIVM
                     ERMDGARVTGPQIAWNKAFTAAGHKRSTHLFNTPPSGPALPGNEAFGIQFSFDGKFAV
                     FVGGYPVVVNGEVVGGVGLSGGNGEQDTACGVAALAALQTLLAGKADVLVQADIKK"
     gene            3214..4206
                     /locus_tag="VGC69_10710"
     CDS             3214..4206
                     /locus_tag="VGC69_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0295792.1"
                     /translation="MSHRVLWLQTQQSFDVEDGETVLAAAQRQEVDLPHECTFGGCGT
                     CRMAIVQGAVRYEAPPLSLTEAEHAQGQGLACQALCASDLVISIETAPAVSDPVLMEA
                     TIEAVSALAPGITLLRLQLPPEHGVSYLPGQYLNIHLEDGARRSFSIANAPCEQRIDL
                     HIRRIEGGRFTGRMLQDIKAGDTLRVELPHGAFYYREQDYQPMIFAATGTGLAPIKAI
                     LESLLDHDDLPPCRLYWGMSSQDDLYHLDELAQWGKRLYEFEFVPVLSRPAPGWQGRT
                     GYVQDAVLADVPDLAEHSVYLCGSPDMINDAKARFVQAGADPAHLYADSFTFQT"
     gene            complement(4203..4601)
                     /locus_tag="VGC69_10715"
     CDS             complement(4203..4601)
                     /locus_tag="VGC69_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0295793.1"
                     /translation="MTDRYSIGSLSRRSGVNAETIRYYEKVNLLPAASRAGNGYRQYG
                     DASLERLAFIRRGRELGFTIDEIRELLTLAHHPGHPCSDADRMTHAHLEDIEGKIRDL
                     QRMRRALRQVADCAGQTAERCELLRALAAA"
BASE COUNT          906 a         1720 c         1425 g          754 t
ORIGIN      
        1 acttgcccat gccgcactgg taggccagat gcggaaaagg aaactgcttg atgcccgcgc
       61 ggacaaccag gttggcgccc tctttgacct cgccggcgta ggtctggccg ttcttgtgaa
      121 agatgacgtg gggcatggtg caaagaaact ccaattagta tactgtatac taaattgtct
      181 cgactttcag acgcatcgaa aatcccggat taacccgtat tcgaccccgc tcccgatact
      241 caagcttgcg acaaggcggt tttaccaggg ttttcccgat tccaagacct tgatatcgtt
      301 tctattatgt atacagtatc ccataaatgc cgcgggatac aaggccggag acgctcgctg
      361 acacccgcgg cgcctttcca ctgcacctcc ccctacaaag gatcctgtca tgtccatgct
      421 catgagccga gacgaattcc gcagcgccct cgaaaacgcc atcaagggca agagcgccaa
      481 ccaagcccct ttcagcatcg cctgggccgc aggcaagctg agccgcgccc acctgtcgcg
      541 ctgggccgaa aaccattacc actatgtcgg ccccttcgcc gactatctgg gctacctgta
      601 cgcccgcacc cccgaccaca tgaccgaggc caaggacttc ctgctggcca atatgtacga
      661 agaggaaatc ggcggcgacc gccacaccga cctgctgatc cgctttgccg aagcctgcgg
      721 caccacccgc gaacgcgtga ccaaccccga caatatgtcg cccaccaccc gcggcctgca
      781 ggcctggtgc tatagcgtcg ccatgcgcga agaccccgtc gtcgccgtcg ccgccctggt
      841 cgtgggcctg gaatcgcagg tgccctcgat ctaccgcaag cagaccccca cgctgcgcga
      901 aaaatacggc ttcagcgacg aagaagtcga attcttcgac ctgcacatcg tgtcggatga
      961 aatccacggc gaacgcggct accagatcgt gctggagcac gccgacacgc ccgagctgca
     1021 agcccgctgc ctgaagatct gcgaaatcgg cgcgcagatg cgcctgctct acaccacggc
     1081 gctgtaccgc gactacgtcg ccaacgacgt ttccgaggag gaactgctgc ccgcctgatc
     1141 ggaccctcca tgacggccgg ggcaggcagg cgtccaccac tgccccggcc gtcacctctc
     1201 ccgcccgaca cactcctagc gtgcctcatc gccatgacgg attccatcaa gcattaccag
     1261 aactacatcg acggccagtg gcagccctgc caatccggac gcaccttcga caacatcaac
     1321 cccgccgaca cggctgaaat agtgggccgc ttccaggcct ccagcgccgc cgacgccgaa
     1381 gccgccgtgg ccgccgccgc gaaagccttc cccgcctggc gcggcacgcc catcagcaag
     1441 cgggccagga tactgaccga cgccgccgac tacctcgaag cccatgccga cgaattcgcg
     1501 cgggatctca cccgcgaaga gggcaaggcg ctcggccagg cgcgcgacga gatcctgcgc
     1561 agcgcgcaga ctttccgctt ctacgccgtg gaagggcaga ccttcaccgg cgaaacctat
     1621 ccgcaagacg accccctgat gcacgtctac agccatcgcg aaccgctggg cgtggtcacc
     1681 gtcatctcgc cctggaactt ccccgcctcc attgccgcgc gcaagatcgc gcccgccctg
     1741 atcacgggca acaccgtggt gttcaagccg tcgtccgacg cgccgctgac cggcctgcat
     1801 ttggccgaag cgctggcgca cgccggcgcg cccgccggcg ccgtcaactg cctgacgggc
     1861 cgcgcgggcg acgtgggccc ggtcatcacc acgtcccggg ccgtacgcgc catctcgttc
     1921 accggatcga cgcaggccgg cgcgcagatc caccgcagcg tgcacctgac cacccgcctg
     1981 cagatggagc tgggcggcaa gaacccgctg ctcgtcatgg aagacgccga cctcgacgcc
     2041 gcggtcgacc tggccatcaa gggcggcttc tcgctgaccg gacaggcctg caccggcacc
     2101 agccgcatca tcatcgtgcc ccaggtcaag gaggcctaca cccggaagct gctcgagcgc
     2161 atcaagcgcc tgaagatcgg tcccggcctc gaagccggca tggacctggg cccgctggcg
     2221 accgcccggc aactggacac cgtgctccag tacattgcca tcggcaagca agaagccacc
     2281 cacctgatcg gcggcgaaca cctgcaaggc ggccgctacg gcacgggcta ttacgtggcg
     2341 ccggccgtct tcaccgatgt gaagccgggc atgcgcatcg cccaggagga aatcttcggg
     2401 cccgtgctgg cgatcatcga ggccgccgac ttccaggaag cgctcgccat cgccaacgac
     2461 tccgaatacg gcttgtcggc cgccattgcc acgcgcaatc cgcgcaccat gcacctcttc
     2521 gcgcaggaca tcgaatccgg cacggtcaag atcaaccgca ccaccaccgg caacctggtc
     2581 aacgcgccct ttggcggcct gaaaaactcc agcacctcga cgttccgcga gtcgggccgc
     2641 gccgggctgg atttcttcac gcagatcaag accatctatc gcggcgtgta ggccgcggcg
     2701 catccccttc ttcacggaca ttcccatgaa agcagcccaa accctcgaac tgcacgaagc
     2761 gcgcgccatg gcgcaggccg ccctggaaaa ggccagggaa atcggcgtga ccgaaaccgt
     2821 ctgcatcacc gatgcgggcg gctttcccat cgtcatggag cgcatggacg gcgcgcgcgt
     2881 cacgggcccg cagatcgcct ggaacaaggc cttcaccgcc gccggtcaca agcgctcgac
     2941 ccacctgttc aacaccccgc ccagcggccc cgccctgccc ggcaacgaag ccttcggcat
     3001 ccagttcagc ttcgacggca agttcgccgt gttcgtcggc ggctatcccg tcgtggtcaa
     3061 tggcgaagtc gtcggcggcg tgggcctgtc cggcggcaac ggcgaacagg acaccgcctg
     3121 cggcgtggcc gcgctggccg ccctgcaaac cctgctggcg ggcaaggccg acgtgctggt
     3181 gcaggccgac atcaagaaat aggccggaac gccatgtcgc atcgcgtgtt atggctgcaa
     3241 acgcagcaaa gcttcgacgt cgaagacgga gaaaccgtgc tggccgccgc gcagcgccag
     3301 gaagtggacc tgccgcacga atgcaccttc ggcggctgcg gcacctgccg catggcgatc
     3361 gtgcaaggcg cggtgcgcta cgaagcgccg cccctgtcgc tgaccgaagc agaacacgcg
     3421 caagggcagg gcctggcctg ccaggccctg tgcgcgagcg atctcgtcat cagcatcgaa
     3481 accgccccgg cggtctccga ccccgtgctg atggaggcga cgatcgaggc ggtctcggcg
     3541 ctggcgcccg gcatcaccct gctgcgcctg caactgccgc cggaacatgg cgtgagctat
     3601 cttcccggcc agtatctgaa catccatctg gaagacggcg cgcgccgcag cttttccatc
     3661 gccaacgcgc cctgcgaaca acgcatcgac ctgcacatcc gccgcatcga aggcggccgc
     3721 ttcacgggcc ggatgctgca ggacatcaag gcaggcgaca ccctgcgcgt ggaactgccg
     3781 catggcgcct tctactaccg cgaacaggac taccagccca tgatcttcgc cgccaccggc
     3841 accgggctgg cgccgatcaa ggccatcctg gagtccctgc tcgaccatga cgacctgccg
     3901 ccttgccgcc tgtattgggg catgagcagc caggatgatc tctaccatct ggacgaactg
     3961 gcgcaatggg gcaagcgctt gtacgaattc gaattcgtgc ccgtgctgtc gcgccccgcg
     4021 cccggctggc aaggacgcac cggctacgtg caggacgccg tgctggccga cgtgcccgac
     4081 ctggccgagc actccgtcta cctgtgcggc tcgcccgata tgatcaacga cgccaaagcg
     4141 cgcttcgtgc aggcgggggc cgacccggcg cacctctacg ccgacagttt caccttccag
     4201 acctaggcgg cggcaagcgc ccggagcagt tcgcagcgct ccgccgtctg gccggcgcaa
     4261 tcggctacct gcctgagcgc gcgccgcatg cgctgaagat cgcggatctt gccctcgatg
     4321 tcctcgagat gggcgtgagt catccggtcg gcgtccgagc aaggatgccc ggggtgatgc
     4381 gcgagcgtga gcagttcgcg gatctcatcg atggtaaagc cgagctcccg gccgcggcga
     4441 atgaaggcca ggcgctccag cgaggcgtcg ccgtattgcc ggtagccgtt gcctgcccgg
     4501 ctggcggcgg gcaacaggtt cactttttcg taatagcgga tcgtttcggc gttgacgccc
     4561 gagcgtcggc tcaggctgcc gatggaatac ctgtctgtca tggcttgacc ctgtagtggc
     4621 tacagggttc acgatacgcc tttcaagagt cgcgaggaac cgccatgcca ggacactgct
     4681 gccaccattg cgaggatcaa tccggcccgc cgcgggctgg ctatcgcaga ttgctctgga
     4741 tcgcgctggc gatcaacgtc gccatgttcg ccgtcgaagt ggcgacgggg ctgcatggcg
     4801 gatcc
//
LOCUS       DASWPO010000206         6268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2010105, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000206 DASWPO010000000
VERSION     DASWPO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010105"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..837)
                     /locus_tag="VGC69_10720"
     CDS             complement(64..837)
                     /locus_tag="VGC69_10720"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEY0295794.1"
                     /translation="MIRSWLDLPTPGLFGVLCVLYFGIAALLTFVVFHSPFKRPVQSL
                     IGIVAPYFNSVAILFALLTGFLANDVSDRNRQSAHAVQAEASELRNIYTLSVASESDM
                     NTIRAKWRAYVDSVVSQEWPAMAGNHLSMQTYAAYDDLLKEVSTPAIAQTAGQAVSAA
                     LLGATVRVGTARSDRLALASGTTNDMKWLVVIILGIFTQLTIALVHLERPRAFITALV
                     VFSSAAIVALGTIALQEYPFSGPLRISPDPIVALQALGQ"
     gene            complement(1216..1291)
                     /locus_tag="VGC69_10725"
     tRNA            complement(1216..1291)
                     /locus_tag="VGC69_10725"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1256..1258),aa:Phe,seq:gaa)
     gene            1459..2733
                     /gene="hemA"
                     /locus_tag="VGC69_10730"
     CDS             1459..2733
                     /gene="hemA"
                     /locus_tag="VGC69_10730"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY0295795.1"
                     /translation="MSVAVFTFGLNHTSAPVSVRERVSLPEDLLKPALAGLRAAFGGA
                     VREAAILSTCNRTEVYCAAEAQAAEQLPCWLAEHHRLETNVLRPHLYRHDKDAAVRHA
                     FRVASGLDSMVLGEPQILGQMKDAVRAADEAGSLGTLLHQLFQRTFSVAKEVRSQTAI
                     GAQSVSMAAAAVRLAERVFGKLDQARVLFIGAGEMIELCATHFAAQQPQCMVVANRTV
                     ERADALAGRFNAGTMRLADLPERLADFDVIVSCTASSLPILGLGLVERAIRQRRHRPM
                     VMVDLAVPRDIEPEAGRLADVYLYSVDDLGRMVQSGTDARRAAVVQAESIIDTRVQGF
                     MHWLQSREMVPVIHDLQQAAADVQAAELDRARRMLARGESPEAVLEQLAHGLTQKYLH
                     GPLAALNRSEGDDRRQLLAMVPRLFPGKDARR"
     gene            2824..3906
                     /gene="prfA"
                     /locus_tag="VGC69_10735"
     CDS             2824..3906
                     /gene="prfA"
                     /locus_tag="VGC69_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY0295796.1"
                     /translation="MKASMRGRLEQLAHRLIEVDALLADPDSASDMDRFRRLSRERAD
                     IEPVAEAFRHFERTEADLAAAQEMLGDPEMRDMAEEEIKAGRARVEQLEADLQLLLLP
                     KDPDDGRSLFLEIRAGTGGDESALFSGDLLRMYTRYAERQGWRVELMSESASELGGYK
                     EVIARIDGEGAYGRLKFESGAHRVQRVPATEAQGRIHTSACTVAVMPEADEMGAIVIN
                     PNDLRIDTFRASGAGGQHINKTDSAVRITHLPTGLVVECQDDRSQHRNKDRAMQVLAA
                     RLKDKEQRERQSKEAAERKSLIGTGDRSERIRTYNFPQGRVTDHRINLTLYKLLQIME
                     GDLDELTGALIAEHQAEQLAALGEQV"
     gene            3983..4723
                     /gene="prmC"
                     /locus_tag="VGC69_10740"
     CDS             3983..4723
                     /gene="prmC"
                     /locus_tag="VGC69_10740"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY0295797.1"
                     /translation="MLDKPRAWLLTHDTDEAEPRAQAAYFALAERRLAGEPVAYLVGE
                     REFMGHMFRVTPDVLIPRPDTELLAETAIALLAGRQGARALDLGTGSGAIAVSIALAC
                     PHVEVGATDRSADALAVAADNARRLGAKVHCWQGDWYEALPEGERFDVIVSNPPYIAR
                     DDAHLGQGDLRFEPRGALTDGGNGLADLASIVAGAGKRLNPGGALWLEHGWEQAEIVR
                     DMLAQAGFAGVNSHKDLAGIERISGGNL"
     gene            4829..5155
                     /gene="grxD"
                     /locus_tag="VGC69_10745"
     CDS             4829..5155
                     /gene="grxD"
                     /locus_tag="VGC69_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY0295798.1"
                     /translation="MSDIQDFIRETVTTHPVVLFMKGTAQFPQCGFSSRAIQILKGNG
                     VKKLVTVNVLEDDEVRQGIKEFSSWPTIPQLYIQGEFIGGSDIMNEMNENGELKQLLE
                     ASGATV"
     gene            5224..>6268
                     /locus_tag="VGC69_10750"
     CDS             5224..>6268
                     /locus_tag="VGC69_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0295799.1"
                     /translation="MTDLSTSGIPAYADAVAGFDLAAEAARLHGDLERGVNACVECCD
                     RYAGTGATALDYIDTEGRHQAFSFAQMQALAARVANLLASQGVRPGDIVAGLLPRTPE
                     ILATILGTWRAGAVYQPLFTAFGPKAIEHRLRMSQAKLVVTNTANRAKLDEIGHCPPV
                     AVVLKPGESLRDGDIDFQDALARQSEVFEPVLRKGTDLFMMMSTSGTTGLPKGVPVPL
                     RAMLAFAAYMRQAVDLRPGDRFWNIADPGWAYGLYYGITGPLLLGHGTTFFEGGFTVE
                     STYDLIEKLGITSFAGAPTAYRMLMAAGAQAAKRIKGRLRVVSSAGEPLNPEVIRWFD
                     TAVGVPIHDHYGQT"
BASE COUNT         1098 a         2012 c         2088 g         1070 t
ORIGIN      
        1 tccctttacg aattcaggcc gacattgact gcttcgcgtg ccatgccggg agtgcgccca
       61 tcactattga cccagcgcct gcagcgccac gatgggatcc ggtgaaatcc gcagcgggcc
      121 actgaacgga tattcttgca aggcaatggt tcccagcgcc acgatggcag cactggagaa
      181 caccaccagc gcggtaatga aagcgcgtgg gcgctcgaga tggacaagcg cgatcgtgag
      241 ttgcgtgaaa atgccgagga tgatcaccac cagccatttc atatcgttgg tggtgcccga
      301 tgccagtgcc agccggtcgc tacgagctgt gccgacacgt acagtcgccc ccagcagcgc
      361 ggcgctaacg gcttgtccgg cggtttgggc gatggccggg gtactgactt ccttcagcaa
      421 atcgtcgtaa gccgcgtagg tctgcatgga gagatgattg cccgccatcg cgggccattc
      481 ttggctcacg accgagtcga catacgctct ccatttcgcc cggatggtgt tcatgtccga
      541 ctcggatgca acactgagcg tatagatatt tcgcaattcg ctggcttcgg cctgaacggc
      601 gtgcgcggac tgtcggtttc tatcgctgac gtcattggcg aggaatcccg tcagcaacgc
      661 gaacaatatg gcgaccgagt tgaaatacgg ggcgacaatg ccgatgagcg attgcacagg
      721 acgtttgaac ggcgaatgaa acaccacgaa agtcagcagc gctgcaatac cgaaatagag
      781 cacacacaac acgccaaata ggccgggcgt gggcaaatcc aaccaggatc tgatcatgct
      841 tttcctcatt cataaaatat atttgcgcga gtctcgccct ttcgtccctg gcgcctacgg
      901 cccgtcttgc ctgatcctga cctgcgcgct tcttccgtca agggtggcgg ccggccccgt
      961 gtcctcgcct gctcctgcgg gccgggcttg gccgctctga tctgcatttc gttgagcagt
     1021 cccatgagac ttgtaccccc agaggccgct tttcgagaaa ccattctggg gtacaggttg
     1081 aggtacaacc aagcgtggga tgtaacggga tgacttgggg cggcatgact tgggacgtta
     1141 cgggtgagct ttctcaccta agtccctgat gcacaaatga aaatgcccag ctttcagggc
     1201 tggacatcga atcgctggtg gcctggggcg gaatcgaacc accgacacgc ggattttcaa
     1261 tccgctgctc taccgactga gctaccaggc caacgaagtc cgccaatata ccaaagaatt
     1321 tgtctttttg ccaaagccac ccaagagcca aatccaggcg cggcggctgg ggatatcgac
     1381 taagtccttg ccctgcaagg aattccattt ctcccgcggg cggtataatg aaaacctccc
     1441 ccgcagcccc cgcctgccat gtctgtcgcc gtttttacct ttggcctgaa ccacacctcc
     1501 gcgccggttt ccgtgcgtga gcgggtgtcg ctgcccgagg acttgctcaa gcccgcgttg
     1561 gcgggtctgc gcgcggcctt cggcggcgcg gtgcgcgagg cggcgatcct gtccacctgc
     1621 aaccgcaccg aggtgtattg cgccgccgag gcgcaggcgg ccgagcagct gccgtgctgg
     1681 ctggccgaac accaccggct cgagacgaac gtgctgcggc ctcatctata ccgccatgac
     1741 aaggacgccg ccgtgcgcca tgccttccgc gtggccagcg ggctggattc catggtgctg
     1801 ggcgagccgc agatcctcgg ccagatgaag gacgccgtgc gcgccgccga cgaagcgggg
     1861 tcgctgggca cgctgttgca tcagttgttc cagcgcactt tctcggttgc caaggaagtg
     1921 cgctcgcaga ccgccatcgg cgcccaatcg gtgtccatgg cggccgccgc ggtgcgcctg
     1981 gccgagcggg tgttcggcaa gctggatcag gctcgcgtgc tgttcatcgg cgcgggcgag
     2041 atgatcgagc tctgtgccac gcatttcgcc gcccagcagc cccagtgcat ggtggtggcc
     2101 aatcgcacgg tcgagcgcgc cgatgcgctg gccgggcgtt tcaacgcggg cacgatgcgg
     2161 ctggcggatc tgcccgagcg cctggcggat ttcgacgtga tcgtgtcctg tacggcgagc
     2221 tccctgccca tcctgggcct gggcctggtc gagcgcgcca tccggcagcg ccgccatcgc
     2281 cccatggtga tggtcgacct ggccgtgccg cgcgacatcg agcctgaagc cggccgcctg
     2341 gccgacgtgt atttgtattc ggtggatgat ctgggccgca tggtgcagag cggcaccgat
     2401 gcgcgacgcg ccgcggtggt gcaggccgag tccatcatcg acacgcgggt gcagggcttc
     2461 atgcactggc tgcagtcgcg cgagatggtg ccggtgatcc acgatcttca acaggccgcc
     2521 gccgatgtgc aggcggccga gctcgaccgc gcccgccgca tgctggcgcg cggcgaatcg
     2581 cccgaggcgg tgctcgagca attggcgcat ggcctgacgc aaaagtacct gcacggtccg
     2641 ctggcggcgc tcaaccgcag cgaaggcgac gatcgccggc aactgttggc gatggtgccg
     2701 cgcctgtttc ccggcaaaga cgcccgccgt tagcggcgcc gtccgtcttc cgccccatga
     2761 gcggcggatg cttgccgcgc gagcgcccgc cttgtcgttg tccctttctt ggtatttccc
     2821 ctgatgaaag cttccatgcg cggccggctg gagcagctgg cgcatcgcct gatcgaagtg
     2881 gacgccctgc tggccgaccc cgattccgca tccgatatgg accggttccg caggctgtcg
     2941 cgcgagcgcg ccgacatcga gccggtggcc gaggcgttca ggcattttga gcgcaccgag
     3001 gccgacctgg ccgccgcgca ggagatgctg ggcgatcccg aaatgcgcga catggccgaa
     3061 gaagagatca aggccggccg cgcgcgcgtg gagcagctcg aggcggacct gcagttgctg
     3121 ctgctgccca aagatccgga cgatggccgc agcctgttcc tggaaatccg cgcgggcacg
     3181 ggcggcgacg agagcgcgct gttctcgggc gacctgctgc gcatgtacac gcgctatgcc
     3241 gaacggcagg gctggcgcgt ggagctgatg tcggaaagcg cgtcggaatt gggcggctac
     3301 aaggaagtga tcgcgcgcat cgacggcgag ggcgcctacg gacggctgaa gttcgagtcg
     3361 ggcgcgcacc gcgtgcagcg cgtgccggcc acggaagcgc agggccgcat ccatacctcg
     3421 gcctgcacgg tggccgtcat gcccgaggcc gatgaaatgg gcgcgatcgt gatcaacccc
     3481 aacgacctgc gcatcgatac cttccgcgcc agcggcgcgg gcggccagca catcaacaag
     3541 acggactcgg ccgtgcgcat tacccacctg cccacgggcc tggtggtgga gtgccaggac
     3601 gaccgctcgc agcaccgtaa caaggaccgc gcgatgcagg tgctggccgc gcgcctgaag
     3661 gacaaggagc agcgcgagcg ccagagcaag gaggcggccg agcgcaagag cctgatcggc
     3721 acgggcgacc gttccgagcg catccgcacc tacaacttcc cgcaagggcg cgtgacggat
     3781 caccgcatca acctcacgct gtacaagctg ctgcagatca tggaaggcga cctggacgag
     3841 ctgaccggcg cgctgatcgc cgagcaccag gccgagcagt tggccgccct gggcgaacag
     3901 gtctgaagcg atgacgacca tccgcgacct gctccttggc gccggcctgc cccgcctgga
     3961 ggcgcgcatg ctgctctagc acgtgctgga caagccgcgc gcctggctgc tgacgcacga
     4021 caccgacgag gccgagccgc gggcgcaggc ggcgtatttc gcgctggccg agcggcggct
     4081 ggcgggcgag cccgtggcct acctggtggg cgagcgcgag ttcatgggcc atatgttccg
     4141 cgtgacgccc gatgtgctca ttccccgtcc cgacacggaa ttgctggccg agacggccat
     4201 tgccctgctg gccggccggc agggcgcgcg ggcgctggac ctgggcacgg gcagcggcgc
     4261 catcgcggtg tcgatcgcgc tggcctgtcc gcatgtcgag gtcggcgcca cggaccgcag
     4321 cgccgacgcg ctggccgtgg cggcggacaa tgcgcgccgg ctgggggcga aggtgcattg
     4381 ctggcaaggc gactggtacg aagcgctgcc tgagggggag cgctttgacg tgatcgtgtc
     4441 caatcccccc tatattgccc gcgacgacgc gcacctgggg cagggcgacc tgcgcttcga
     4501 accacgcggg gcgctgaccg atggcggtaa cggcctggcc gacctggcca gcatcgtcgc
     4561 aggggctggc aaacgcctga atccgggggg ggcgctctgg ctggagcacg gctgggagca
     4621 ggccgagatt gtccgggata tgcttgcgca ggccggcttt gccggcgtga atagccacaa
     4681 ggacctcgcg ggcatagagc ggatttccgg cggaaatcta taatcaagct atagacatag
     4741 ctgtagtaat cgtggtggca tgatgctgcc gtgagccgag gaccctgcgc agggcagcct
     4801 cggcggctca agaacttaaa ggacctccat gagcgacata caagacttca tccgtgaaac
     4861 cgtgaccacc caccccgtgg tgctgttcat gaagggcacg gcccaattcc cgcagtgcgg
     4921 cttttccagc cgcgccatcc agatcctcaa gggcaatggc gtgaagaagc tggtcaccgt
     4981 caatgtgctc gaggacgacg aagtccgcca gggcatcaag gaattctcca gctggcccac
     5041 cattccccag ctctacatcc agggcgagtt catcggcggc tcggacatca tgaacgagat
     5101 gaacgagaac ggcgagctca agcagttgct ggaagcttcg ggagccacgg tctgaccgtg
     5161 ctttcctgat tcgttgcaaa cgaccatgcc gcatcgagca tggcgaaaaa ggtaggagac
     5221 atcatgacgg acttatccac ttccggcatt cccgcctatg ccgatgcggt tgccggtttc
     5281 gacctggctg ccgaggctgc gcggctgcat ggcgatctcg agcgcggcgt gaacgcctgc
     5341 gtcgaatgtt gcgaccggta tgccggcacg ggcgccaccg cgctcgatta catcgacacc
     5401 gagggccgcc accaggcctt cagctttgcg cagatgcagg cgcttgcggc gcgcgtggcc
     5461 aacctgctgg ccagccaggg cgtgcggccg ggcgacatcg tggcgggcct gctgccgcgc
     5521 acgccggaaa tcctcgccac gatcctgggc acctggcgcg cgggcgcggt gtaccagccg
     5581 cttttcaccg ccttcgggcc gaaggcgatc gagcaccggc tgcgcatgag ccaggcgaag
     5641 ctcgtggtca ccaatacggc caatcgcgcc aagctggatg aaatcggcca ttgtccgccc
     5701 gtcgcggtgg tgctcaagcc cggcgaatcg ctgcgcgacg gggacatcga tttccaggat
     5761 gcgctggcgc ggcagagcga ggtgttcgag cccgtcctgc gcaagggcac ggatctcttc
     5821 atgatgatgt cgacctcggg cacgaccggg ctgcccaagg gcgtgcccgt gccgctgcgc
     5881 gccatgctgg cgttcgccgc gtacatgcgc caggcggtcg acctgcgtcc cggggacagg
     5941 ttctggaaca tcgccgatcc gggttgggct tatggcctgt actacgggat caccggaccg
     6001 ctcctgctgg ggcatggcac gaccttcttc gagggcggtt tcacggtcga gagcacttac
     6061 gacctgatcg agaagctggg catcaccagc tttgccggcg cgccgacggc ctaccgcatg
     6121 ctgatggctg ccggcgcgca ggcggccaag cgcatcaagg gccggctgcg cgtggtgagc
     6181 agcgcgggcg agcctttgaa ccccgaggtg atccgctggt tcgacaccgc cgtgggcgtg
     6241 ccgatccacg atcattacgg gcagaccg
//
LOCUS       DASWPO010000207        19357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3172839, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000207 DASWPO010000000
VERSION     DASWPO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 19357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19357
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3172839"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGC69_10755"
     CDS             complement(<1..506)
                     /locus_tag="VGC69_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0295800.1"
                     /translation="MSIHPAVVPIDASRQPPVSGSTGPIIEFDGVDVELGGQRIYDNL
                     GFEVQRGEFLCILGPSGCGKSTSLRVMGGLLPISAGRVSVAGLPPSEAWAEISFVFQS
                     PRLVSWRSALDNIVLACELRFGKGSLPQRRARAAELLALVGLAGDAAKYPSMLSGGER
                     QRVAIARAL"
     gene            891..1316
                     /locus_tag="VGC69_10760"
     CDS             891..1316
                     /locus_tag="VGC69_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295801.1"
                     /translation="MADAWQFQIRINVSADLAASLRNDPTAAGHPTIRDVLRAHNATV
                     KSQFDAFAEYVSEAERTGKENFPLYRWTKETIGNPEKKTKYLQVFTVSVDGREIYGED
                     AADPLQAQLSALGEDAGIKSVSKLDSNPANNPQPPRRFQ"
     gene            1388..1900
                     /locus_tag="VGC69_10765"
     CDS             1388..1900
                     /locus_tag="VGC69_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY0295802.1"
                     /translation="MPFYVALLRAVNVGGTGKLPMSELKALCLAEGFTQVATYIASGN
                     VVFSADRAETEVKAALERRMQAYAGKPVGVIVRTAKEIADVLKANPFPDEPPNWTVAI
                     FLDSAPSADALNTVQNRKDERIGLGKREIYVAYGAGMGRSRLKILEAANGTARNINTI
                     AKLAEMAAAK"
     gene            2031..2909
                     /locus_tag="VGC69_10770"
     CDS             2031..2909
                     /locus_tag="VGC69_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0295803.1"
                     /translation="MPPISYVRAADGMLLAHYEWRPDPGAPSPLPGPGTPSVYLLHGL
                     SEHAGRYALLARRLNALGWHVGAHDHRGHGRSGGPHAMLRTQDDLVTDAAERLRAWTQ
                     RHGRPPVLLGHSLGALVATRIALYRHAELAGLVLASPPFKVRVPRWSRPALTWLAMRY
                     PDARLWHGRSPKRISHDRRIVRAFRKDPLAHRYLSGRLAAFIDQGGREALRDAPLLDC
                     PTLLLAAGDDRIVAPEGSRLFAMRAPADRLTLRWYDHAWHELFNETPELAEPVHADLT
                     HWLTRLGGPARQNPCL"
     gene            2986..4560
                     /gene="gshA"
                     /locus_tag="VGC69_10775"
     CDS             2986..4560
                     /gene="gshA"
                     /locus_tag="VGC69_10775"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY0295804.1"
                     /translation="MTDSLRYARLAAHACLLDETLRGIEKEGLRVDSRGALSLTPHPA
                     VLGSALTNEQVTTDYSESLLELITGTHRRVDSLIGELTETHRYVCGALGPELIWNQSM
                     PASLPDEAGIPIAWYGTSNSGMLKHVYRRGLAERYGKAMQCIAGVHYNFSLPEALWMA
                     LDTADDDARAHRNRGYIGLIRNFTRYAWLLMYLFGATPALSRDFLRGRGHAMETLDAD
                     TLYLPHATSLRMSDLGYQNKAQSQLKLVYNDLETFLARQYTAVTQPWPAYEAIGTHRD
                     GQWIQLNTNVLQIENEYYSIIRPKRATGRGERPITALAERGVQYVEARCLDIDPWAPA
                     GISAETARFVDAFLLFCAAADSPPFPEDGRCQRSADNFATVVKRGRQPGLMLDRDGTP
                     IGLRQWGEELIDRIAPYAALYDQTQGGSAYAETLAAQRAKLADLALTPSARLLREIRE
                     SGLSFHDYTLRQSRAHAEALRAQPLPADRHAFYVEAARQSVAAQAKLEASDKVDFDTY
                     VANYHAALKNPLPDQG"
     gene            complement(4612..5145)
                     /locus_tag="VGC69_10780"
     CDS             complement(4612..5145)
                     /locus_tag="VGC69_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295805.1"
                     /translation="MRFPPLRGPLRRALRGAVIIAVLTTVMLFGFPDQARHFMQTLSG
                     EADEPDPISVYPYNFTPYGIEYVISPGLLGYLPPGLADGKLMLKQGYVPAAADKGSVP
                     VRWRYVTGEYASASRDTYTFGASLALSSPSKGDVLTLRFYPDGKAAARYTAHPEVGAA
                     GNVDPALPGPGWKTNKP"
     gene            complement(5570..7645)
                     /locus_tag="VGC69_10785"
     CDS             complement(5570..7645)
                     /locus_tag="VGC69_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0295806.1"
                     /translation="MAILRILMVEDSEDDSVLILEALGAAGYEVRALRVDAEEDLRRA
                     LREGQWDLVLSDYFLPGLDARGVLATLKDMGADIPAIVISGTVGEDVAVETLQLGAQD
                     YLLKQNLTRLVPAVARVLEMAENGRRQSKLEHMKTVILENSLDLIGTLDMQGRFLEIN
                     PASQAILGYAPAELTGRVFISLAHPDEFAAILSGFQAVLRGERIRDFECRLIHKSGQD
                     VHLVWSAVLSKADEVIVLVGRDVTERKGIELRLRHLATHDALTGLPNRGLIQEEILQA
                     IKRARDTGQPLALLYLDLDRFKVINDAYGHPFGDAVLKAVGEMLVALVGEKGAVARHG
                     GDEFLILLGNIQRADEAHAISVQIIESLDRPLVVQGRELYLSSSVGVSVYPQDGETPD
                     ELIDNADTAMYRAKEAGRNNYQFFTWKMSAEIQRRVELETRLRGAVAAGQLRLVYQPK
                     VSLESGGIIGCEALLRWHHPDLGAVSPVHFVPVAEDSGLIVPIGDWVLRTACAQAKAW
                     LDAGLPPVCMAVNLSTRQFLQQDVATWVMRTLADTGLPADQLELELTESLIAQDAEKV
                     IATFSRLKDIGVKLSIDDFGTGYSSLGYLKRFRVDTLKIDQSFVHNMFIEPTDATIIL
                     GIISLGHNLRFKVIAEGVETEQHCRFLSQHACDEIQGYYFSQPVPAEEFAAMLGSDKR
                     LPDAAMRPA"
     gene            complement(7659..8081)
                     /locus_tag="VGC69_10790"
     CDS             complement(7659..8081)
                     /locus_tag="VGC69_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0295807.1"
                     /translation="MKNKTILLVEDNPDDEELALLAFRQMDVDARIVVARDGVEALDL
                     LFGPDGRGADVLPSVVLLDLNLPRVDGFEVLDRIRGGDRTRFLPVVILTSSREERDFI
                     ESYSRGCNSYVRKPVDFAEFMQAVRQLGLYWLSLNETP"
     gene            complement(8078..10459)
                     /locus_tag="VGC69_10795"
     CDS             complement(8078..10459)
                     /locus_tag="VGC69_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0295808.1"
                     /translation="MNSQPQANLSDADPRAATAVLPMSFSRALIGNVVGLVVMLGLVL
                     LGALAWQGIASTEKAERRDIAQTLNSSTERLQLLTRAAEMTADSAVRASLVSAVTGAT
                     LRSTLEQALAAFEQRPELSYLGIILPDAGEYGTLERTAKGEILLWLFPGTLANDPVTR
                     IFTLTDRGFVLRESRPYDGYDPRGRPFYQAALAAAPGGTWMRAYPWIIHDEHSEPLWG
                     FSYVRALRDQEGRLVSVLDTDFDLPAFNAFLKSIADEYHARLQVVELGETPRLIGGAG
                     VGRTPLPLNAELAPLIKSTDAVFTARMALEGERRWVAARRVTLPGGPTWLVVASRDAP
                     LMEASLRRQLYQLAGMGLVVVAGLALLSARMARHFGRPLAELERRLDRIERHGLQAPE
                     PGLSVVDAFRETQLLGKALDRMSVAVRQQVLAKEQQLASIALKAAIFDHTNTAIFTLD
                     ARGVIIEWNAVAEQQFGLARAGALGQPVAQAIRAPDGPADWDAIQASQGAGTFTLLGE
                     QGPFEAEARAVAFEQDGLQVRTFFLNDVSEYKRAQARLLVFNAELEQRVAHRTRELRA
                     LNDELESFCHSVSHDLRAPLRGISGFTEILAKNHAGALDEKARGYLGRVQLAACRMDE
                     LIDDLLKLSRVSREEMHREKVDLSAIARQVLADLRQAEPQRRVDISIEDGLAAEGDAR
                     LLRIALENLLGNAWKFTSKTPDARISFAAMPGENGEPGFAVADNGAGFDMRYARRLFA
                     PFQRLHRAVEFPGTGIGLATVQRIVKRHGGYIAAQAEPGRGARFWFSLDQAAS"
     gene            complement(10832..10906)
                     /locus_tag="VGC69_10800"
     tRNA            complement(10832..10906)
                     /locus_tag="VGC69_10800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10871..10873),aa:Arg,seq:ccg)
     gene            complement(10929..12446)
                     /locus_tag="VGC69_10805"
     CDS             complement(10929..12446)
                     /locus_tag="VGC69_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY0295809.1"
                     /translation="MQEIQTIGIVGAGAMGRGIAQIAAQAGLRVRLYDASAEAVAAAR
                     EALRQTWDKLAQKGKLSEADAQAALARVEPAAGLDALADCQAVVEAIVERLDIKREVF
                     TRLEAIVAADCILASNTSSLSITAIAAACKQPARVAGYHFFNPVPLMKVVEVIDGLRS
                     SPAVGDALMALARRMGHTPVRAKDMPGFVVNHAGRGMNTEGLRVAQESVAGFDQIDAI
                     MREQAGFRMGPFELLDLTGLDVSHPVMESIYQQFYDEPRYTPSPITAVRQAGGLLGRK
                     TGEGFYAYVDGRKQVPAEPAVPALPAGLKVWVSRHSSQGHARAAALLSGLGATLSEKS
                     EAGVLCIVTPYGQDVSTCIAQQGLEADRTVGLDTLHELAPGRRRTLMLSPGTLPSWRD
                     AAHALLAGDGAPVSVIADSAGFVAQRIVAHIVNIACDIAQQRIATPEDIDRAVTLGLG
                     YPMGPLAMGDALGAGRIMEILVNMQRVTGDMRYRPSLWLQRRVQLGLPLLAQPAA"
     gene            complement(12476..13720)
                     /locus_tag="VGC69_10810"
     CDS             complement(12476..13720)
                     /locus_tag="VGC69_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0295810.1"
                     /translation="MSVQSAPQRPAPLTGIRVLDLTRVLAGPWCTQNLADLGAEVVKI
                     ERPGAGDDTRAWGPPYLKDQDGNPTTEAAYYLSANRNKLSVAVDIATPRGAEVVRELA
                     GQSDIFVENFKAGGLRKYGLDYESLRQINPRLIYCSVTGFGQTGPYASRPGYDFMIQG
                     MGGLMSITGERDDLPGGGPQKAGVAVADLMTGMYSTVGILAALIERGHSGLGQHIDMA
                     LLDCQVAMLANQNLNFMTSGVAPRRAGNAHQNLVPYQVFAAADGHLIVAVGNDSQFRN
                     YCRVIGMTELAEDPRFATNPQRVINREALVPILVERMKTGARDQWLAELEAAGVPAGP
                     INTLEQVYQDPQVLARNMKLELPHPTAGVAPIAASPLKLSASPVRYDRPAPMLGQHTH
                     EVLRERLGLSDADIEALSRPAD"
     gene            13802..14656
                     /locus_tag="VGC69_10815"
     CDS             13802..14656
                     /locus_tag="VGC69_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0295811.1"
                     /translation="MTISTPILRDQLPALFTKRESDTNKGSFGTLGVLGGGPGMTGAA
                     LLAARAALRLGAGKVLVGFAQGALPLACDPLQLELMLRTGRDLITDAGALGVNAWVAG
                     CGIGATADAARLLAALFQARKNAPLVLDADGLNLLAQGALQADWGPAPVVLTPHPAEA
                     GRLLDCTAAQVQADRPAAAQALAQRYQAWIVLKGAGTLVCPPGGGDCLVNTTGNPGLA
                     SAGTGDVLAGMLGSLIAQGVPLAQAVPGAVWLHGAAADALVAAGIGPIGLTAGELAEA
                     ARRLRNAP"
     gene            14817..15041
                     /locus_tag="VGC69_10820"
     CDS             14817..15041
                     /locus_tag="VGC69_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295812.1"
                     /translation="MNTTNEFRLTDELERALLREAMVTPREVELLPALKSGLAAIGRA
                     FDSVFAFIGNLNDAMNQARARSATYSGSQW"
     gene            15271..16173
                     /locus_tag="VGC69_10825"
     CDS             15271..16173
                     /locus_tag="VGC69_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0295813.1"
                     /translation="MKPADLSNADRLALELGHAGRNDFAYLDEDRNADRPITLNTYRP
                     YGYTPDRPVVIVQHGVMRNGDDYRDFWIPAADQHKLLIVAPTFSDAVWPGVESYNNGL
                     AYTAMGNPRHVDGWAYALPGRVVADMVAAELIDGQNTYLFGHSAGGQFVHRLMGSQAH
                     APWKAVAIGNPGWYTLPDMDRKFPEGLGGVGLTEAHLERLLAYPMLILAGDQDIETGG
                     HLPSDPPAMAQGPHRYARAHYYLEAGQAEARRRGIACNWSLRSVPGIGHDGRAMSAAC
                     ASLWFEGRMPDAGALAALASGHVA"
     gene            16243..17373
                     /locus_tag="VGC69_10830"
     CDS             16243..17373
                     /locus_tag="VGC69_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY0295814.1"
                     /translation="MSTLPNTEQLVAGIIAWMQCESPTSSPAGVAAMAEITAGYARQQ
                     GLKAAVTPLGENVGPMLYATNRAEGDTRPGLLILAHMDTVHPVGTLDDNPIRIEGDRL
                     YGPGGYDMKAGTYIALSAMAGLGAPGATALPIDFLMVPDEETGSHASRPQIEKFAAGA
                     KYCLVCEPARADTGMCVTARKGTGMLRLGVKGMPSHAGVAHQKGRSAIKEMAHQVLAL
                     EAITDYDCGITVTVGTIAGGTATNTVPERCRCVVDFRVPDMASADYVLRRMRELCAVG
                     PDTELDIDVELNRPPMVKTASSTALLDHARVFAKEAGFELNDAPMTGGGSDANFTSAM
                     GVPTLDGLGADGDGAHTLKEHILVSTLEQRVRFWHLLLEGLA"
     gene            17445..17672
                     /locus_tag="VGC69_10835"
     CDS             17445..17672
                     /locus_tag="VGC69_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY0295815.1"
                     /translation="MAQITLYLDEDTEILVAQAAKAKGISKSRWVAEAIRQYAHDTWP
                     AECLALAGAFPDFPLREDSPSSGEDLPRIGF"
     gene            17675..18082
                     /locus_tag="VGC69_10840"
     CDS             17675..18082
                     /locus_tag="VGC69_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0295816.1"
                     /translation="MLVLDSNTISYYFRGDECVVPRLQALAPEEVGVPAIVVYELRYG
                     LLRLPSVAAQPRLDALEALLRPMQTLAFDAACAEQAAALRQSLEKTGTPIGPHDVLIA
                     ATALRHHARLVTRNEREFARVPNLQIINWHAAA"
     gene            complement(18116..18259)
                     /locus_tag="VGC69_10845"
     CDS             complement(18116..18259)
                     /locus_tag="VGC69_10845"
                     /inference="COORDINATES: protein motif:HMM:NF023508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HEY0295817.1"
                     /translation="MFSLFFRSLSRLLAWRGPSRHDAYLASAIDLAQLEDRMRVWARS
                     QED"
     gene            complement(18342..18950)
                     /locus_tag="VGC69_10850"
     CDS             complement(18342..18950)
                     /locus_tag="VGC69_10850"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0295818.1"
                     /translation="MQLIGMLDSPYVRRTAIGLKLLGIAFEHRAVSVFSTFEAFQALN
                     PVVKAPTLILDDGQALMDSTLILDYAQSLAKPAASIFPAEPGARARAVRLTGLALAAC
                     EKTMQIVYEHNLRPPEKQHQPWLDRVRGQLLAAYGLLEAAQRTDPLDAEVLDHAGVAV
                     AVAWTFTRMMVPAIVPADDFPALRTFSARCEQLPVFRDTPPV"
     gene            19109..>19357
                     /locus_tag="VGC69_10855"
     CDS             19109..>19357
                     /locus_tag="VGC69_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295819.1"
                     /translation="MNHLPPLPALRYFEAVARLGSVTSAARELHVTHSAVSQQIKLLE
                     DMMGVPLFVRAARGLTLTEEGRLYALEIRAALRDIETAT"
BASE COUNT         3354 a         6701 c         6219 g         3083 t
ORIGIN      
        1 agggcgcgcg cgatggccac ccgctggcgc tcgccgccgg acagcatcga cggatacttg
       61 gccgcgtcgc ccgccaggcc gaccagcgcc aacaattccg cggcccgcgc gcggcgctgc
      121 ggcagcgaac ctttgccgaa gcgcagctcg caagccagca caatattgtc cagcgcgctg
      181 cgccaggaca ccagccgcgg ggattggaag acaaaggaga tctcggccca ggcttcgctg
      241 ggcggcaatc ccgccacgct gacgcggccg gcggaaatcg gcagcaagcc gcccatgacc
      301 ctgagcgagg tggacttgcc gcagccggac ggtcccagga tgcacaggaa ctcgccgcgc
      361 tggacctcga agccgagatt gtcgtaaatg cgttggccgc cgagttcgac gtcaacgcca
      421 tcgaactcga tgatggggcc ggtgctgccg gaaacggggg gctgcctgga tgcgtcgatg
      481 ggcacgactg ccgggtggat cgacatggat cgtcaagtat gcatcagatt gtttacatct
      541 aaactaacta taaggactcg gtttcgttcc aacctatagg tacaaacctg gatggttgct
      601 ttgcaatacg gcatggggtc tctttttttc aggcgtttag cccgtattca gcagcgccgt
      661 cgggcgattt ttttggcggt cccggcaagg gccgctttcg aatcccgtga atttagcatt
      721 ccccacattt cctgatgtgc aggcccggat gcgcgcgggc tgcccggcct gcattcggcg
      781 tggatattat cgaaagccga tgttcccgcc cgtaaggttg cccataaaat aaacgaggct
      841 cttgcgcttg ccgccacatg gcatcgccgc ccctggggag cacatcgaat atggctgacg
      901 catggcaatt ccagataagg atcaacgttt cggccgatct tgcggccagc ctgcgcaacg
      961 accccacggc ggcgggacac ccgacgatcc gcgacgtact gcgcgcgcac aatgccacgg
     1021 taaagtcgca gttcgatgcc ttcgccgagt acgtcagcga agccgagcga accggaaagg
     1081 aaaacttccc gctttatcgg tggaccaagg aaacgatcgg gaaccccgag aaaaaaacga
     1141 agtacctaca ggtcttcacc gtgtccgtgg acggccggga gatctatggc gaggacgccg
     1201 ccgatccttt gcaggcgcag ttgtcggcgc tgggcgagga cgctgggatc aaaagcgtat
     1261 cgaagctcga tagcaatcca gcgaacaacc cacagccgcc caggcgattt caataaaaat
     1321 tgccgaatcc atgtcggctt caggttgacg agggatttca caagattgga aagtaacgag
     1381 gatttgcatg cctttctacg ttgcgttgct gcgcgctgtg aacgttggcg ggacggggaa
     1441 gctgcctatg tccgaattga aggcgttgtg ccttgccgag ggtttcacgc aggttgcgac
     1501 ctacatcgcc agcggtaatg tcgtcttttc agccgacaga gccgagacgg aggtcaaggc
     1561 cgctcttgaa cggcgcatgc aggcctacgc cggaaagccg gtgggcgtca tcgtccgcac
     1621 ggccaaggaa atcgcggacg tcctgaaggc caatccattt cccgacgagc ccccgaattg
     1681 gaccgtcgca atctttcttg actccgcccc gtccgccgat gcgctcaata ctgttcaaaa
     1741 caggaaggac gagaggatcg gcctgggcaa gcgggagatt tatgtggctt acggtgctgg
     1801 catggggcgc tccagactca agattctgga ggccgcgaac ggtactgccc gcaacatcaa
     1861 taccattgca aagctggcgg agatggcggc agccaaataa aaaaagcggc gggccgccag
     1921 gccgctgtcg ccctggcgct acgcctggac cggctttgac ggggccttct tcaacatgaa
     1981 atgaaactga gttgaaatgc gctcatccca actcgagcga ggcttgcccc gtgccgccga
     2041 tctcgtatgt ccgcgccgcg gacggcatgc tgcttgcgca ttacgaatgg cgtcccgacc
     2101 ccggcgcgcc gtctccgctg cccggccccg gcacgcccag cgtctacctg ctgcacgggc
     2161 tgagcgaaca cgccggccgc tatgccctgc tcgccaggcg gctcaatgcc ctgggctggc
     2221 acgtcggcgc ccacgaccac cgcggccacg ggcgctccgg cggcccgcac gccatgctcc
     2281 ggacccagga cgacctggtc acggacgccg ccgagcgtct ccgcgcctgg acccagcggc
     2341 acggccgccc gcccgtgctg ctgggccaca gcctgggcgc cctggtggcc acgcgcatcg
     2401 ccctctaccg ccacgccgag ctggccggcc tggtgctggc ctcgccgccc ttcaaggtgc
     2461 gcgtgccgcg ctggagccgc ccggcgctga cctggctggc catgcgctac cccgatgccc
     2521 ggctgtggca cggccgcagc cccaagcgca tctcgcacga ccgccggatc gtgcgcgcct
     2581 tccgcaagga cccgctggcg caccgctacc tgtcgggccg gctggccgcc ttcatcgacc
     2641 agggcgggcg cgaggccctg cgcgatgcgc cgctgctcga ctgccccacg ctgctgctgg
     2701 ccgcgggcga cgaccgcatc gtcgcccccg agggcagccg gctatttgcg atgcgcgcgc
     2761 cggccgaccg gcttacgcta cgctggtacg accatgcctg gcacgagctt ttcaacgaaa
     2821 cccccgagct cgccgagccc gtgcacgccg atctgaccca ctggctgacc cggctgggcg
     2881 ggccggccag gcagaatccc tgcctgtaac cccggtaagc aacatgacag cggccccggc
     2941 accgtaaaat cgggggccaa cctgcataag catatataag aactcgtgac tgactcccta
     3001 cgctacgccc ggctcgccgc ccatgcctgc ctgctcgacg aaaccctgcg cgggatcgag
     3061 aaggaaggcc tgcgcgtcga cagccggggc gccctgtccc tgaccccgca ccctgccgtg
     3121 ctgggctcgg ccctgaccaa cgaacaagtc accaccgact attccgaatc gctgctcgaa
     3181 ctgatcaccg gcacgcaccg tcgcgtcgac agcctgatcg gcgagctcac cgaaacccac
     3241 cggtacgtct gtggcgccct gggcccggag ctgatctgga accagtccat gcccgcctcc
     3301 ctgcccgacg aggccggcat tcccatcgcc tggtacggca cgtccaactc gggcatgctc
     3361 aagcacgtct accgccgcgg cctggcagag cgctacggca aggccatgca atgcatcgcc
     3421 ggcgtgcatt acaacttctc gctgcccgag gcgctctgga tggcgctcga caccgcggat
     3481 gacgacgcgc gcgcgcatcg caaccgcggc tatatcggcc tgatccgcaa cttcacgcgc
     3541 tacgcctggc tgctgatgta cctgttcggc gccacgcccg cgctgtcgcg cgacttcctg
     3601 cgcggccgcg gccatgcgat ggaaacgctg gatgccgaca cgctctacct gccgcacgcc
     3661 accagcctgc gcatgagcga cctgggctac cagaacaagg cccagtcgca gctcaagctg
     3721 gtctacaacg acctggaaac cttcctggcc cgccaataca ccgccgtcac gcaaccctgg
     3781 ccggcctacg aggccatcgg cacgcaccgc gacggccagt ggatacagct caacaccaac
     3841 gtgctgcaga tcgagaacga gtactactcg atcatccgtc ccaagcgcgc caccggccgg
     3901 ggcgagcgcc ccatcaccgc gctggccgag cgcggcgtgc agtacgtgga agcccgctgc
     3961 ctggacatcg acccctgggc gccggccggc atctcggccg agacggcccg cttcgtcgat
     4021 gccttcctgc tgttctgcgc cgcggccgac agcccgccct tccccgagga cggccgctgc
     4081 cagcgcagcg ccgacaattt cgccacggtc gtcaagcggg gccgccagcc cggcctgatg
     4141 ctcgaccgcg acggcacgcc gatcggcctg cggcaatggg gcgaggagct gatcgaccgc
     4201 atcgccccct acgcggccct gtacgaccag acccagggcg gcagtgccta tgccgagacg
     4261 ctggccgcgc agcgcgccaa actggccgac ctggcgctca cgccttcggc gcgcttgctg
     4321 cgcgaaatac gcgagtccgg gctgagtttc cacgactaca cgctgcgcca gagccgcgcg
     4381 catgccgagg ccctgcgcgc gcagcccttg ccggccgacc gccacgcctt ctatgtcgag
     4441 gcggcccggc agtccgtcgc cgcccaggcc aagctcgagg cgtccgacaa ggtcgacttc
     4501 gacacctacg tggccaacta ccacgccgcg ctcaagaacc ccctgcccga ccaaggttga
     4561 tcgcaggggc tggcgggccc cccccggccg aggggggttc cggcggccgg gtcagggctt
     4621 gttggtcttc cagcccggcc ccggcaaggc gggatcgacg ttgccggccg cgccgacctc
     4681 cgggtgggcg gtgtagcggg cggcggcctt gccgtccggg tagaaacgca gcgtcagcac
     4741 gtcgcccttg gatggggagg acagcgccag ggaggcgcca aaggtgtagg tgtcgcgcga
     4801 cgcgctggcg tattcgcccg tcacatagcg ccagcgcacc ggcacgctgc ccttgtccgc
     4861 ggccgccggc acataaccct gcttgagcat cagcttgccg tcggccaggc cgggcggcag
     4921 atagcccagc agccccgggc tgatcacata ttcgatgccg tagggcgtga agttataggg
     4981 atagacggag atcggatccg gctcgtcggc ctcgccggac aaggtctgca tgaaatggcg
     5041 cgcctggtcg ggaaagccga acaacatgac cgtggtcagc acggcgatga tcaccgcacc
     5101 gcgcagcgcg cgccgcaagg ggccgcgcaa agggggaaac cgcatggaaa cttcctggtt
     5161 tttcttgcaa aagcggaagt tttaacccat gcccccgatc caggggtttc agaactgctg
     5221 caaagaccgc gtcagcaaac ctgccggccg gcgtagccga agggccagcg gcgcggcggc
     5281 ggcacgacgg gcgggggagc cgagtctttg ttcatgatga tctccaaggg agaattcgac
     5341 gacttgagca tagccgcggc cctgggataa accttgaaca ggccagacaa agcgaaacaa
     5401 aatctaaaca gggccgggta gaccttccaa gatgcgtctc gactgtaacg tcatggaaac
     5461 ggattccgcc tggccggcaa gacggatcgg cgctgcgcca cccgcgcgca gggcaagaca
     5521 cgattgttac aaactgggcg caagaccgtg gcggcaatgc cgcacatcgc taggccggcc
     5581 gcatcgcggc gtccggaagc cgcttgtccg aacccagcat ggcggcgaac tcctcggccg
     5641 gaaccggctg gctgaagtaa tagccctgga tttcatcgca ggcatgctgg ctgaggaagc
     5701 ggcagtgctg ctcggtctcg accccctccg cgatgacctt gaacctgagg ttgtgcccca
     5761 gcgagatgat gcccaatatg atggtcgcgt ccgtcggctc gatgaacata ttgtgcacga
     5821 acgactggtc gatcttcagc gtatccacgc ggaagcgctt caagtagccc aggctcgaat
     5881 agccggtgcc gaagtcgtcg atggaaagct tcaccccgat atccttgagc cggctgaacg
     5941 tggcgatcac cttctccgca tcctgggcga tcaggctttc ggtgagctcg agctcgagct
     6001 ggtcagccgg caggcccgtg tccgccagcg tgcgcatcac ccaggtcgcc acgtcctgct
     6061 gcaggaactg gcgcgtggaa agattcaccg ccatgcatac gggcggcagc cccgcgtcca
     6121 gccacgcctt ggcctgcgcg caggccgtgc gcagcaccca gtcgccgatc ggcacgatca
     6181 gcccggaatc ctccgcgacc ggcacgaagt ggacggggga aaccgcgccc aggtccgggt
     6241 ggtgccagcg cagcagcgct tcgcagccga tgatgccgcc gctttccagg ctcaccttgg
     6301 gctggtagac cagccgcagc tggccggcgg ccacggctcc ccggagccgc gtctccagct
     6361 cgacgcggcg ctggatttca gcgctcattt tccacgtgaa gaattggtaa ttgttccggc
     6421 cggcctcctt ggcgcggtac atggccgtgt ccgcgttgtc gatcagttcg tcgggcgttt
     6481 cgccgtcctg gggatagacg ctcaccccga cgctgctgga tagatagagc tcgcgccctt
     6541 gcacgacgag cggcctgtcc aggctttcga tgatctgcac ggaaatggcg tgcgcctcgt
     6601 cggccctttg tatattgccg agcaatatca gaaactcgtc gccgccgtgg cgcgccacgg
     6661 cccccttctc gcccaccagc gcgacgagca tctcgccgac ggccttgagc acggcgtcgc
     6721 cgaacgggtg gccgtaggca tcgttgatca ctttgaaccg gtcgaggtcg aggtaaagca
     6781 gcgcaagcgg ttggcccgtg tcgcgcgcgc gcttgatcgc ctgcaggatt tcctcctgga
     6841 tcaggccgcg attgggcagg ccggtcagcg catcgtgcgt ggccagatgg cgcagcctca
     6901 gctcgatgcc cttgcgctcg gtgacgtccc ggccgaccag cacgattacc tcgtcggcct
     6961 tggaaagaac ggccgaccag accaggtgca cgtcctgccc ggacttgtgg atgagccggc
     7021 actcgaaatc gcgtatgcgc tcgccgcgca agacggcctg gaagccggac aatatggcag
     7081 cgaactcgtc gggatgggcc aggctgatga agacgcggcc ggtcagttcg gcgggggcat
     7141 agcccaatat ggcttgcgaa gccgggttga tctcgaggaa ccggccctgc atgtcgagcg
     7201 tgccgatgag gtccagcgag ttttccagga tgaccgtctt catgtgctcg agcttgcttt
     7261 gccgccggcc gttctcggcc atctcaagca cccgggccac cgcggggacg agccgggtca
     7321 ggttctgctt gagcaggtaa tcctgggcgc ccagctggag cgtttccacg gcgacgtcct
     7381 cgcccaccgt gccggagatg acgatcgcgg gaatgtcggc ccccatgtcc ttcagcgtcg
     7441 ccaggacgcc gcgcgcgtcc aggccgggca ggaaatagtc ggagaggacg aggtcccatt
     7501 gcccttcgcg cagggcccgc cgcaaatctt cttcggcatc gaccctgagc gcgcgcacct
     7561 cgtagccggc cgcgcccaac gcctcgagaa tgagcaccga gtcgtcttcg gaatcctcga
     7621 ccatgagaat gcgcagtatc gccatagcct ggagtgtcct agggagtctc gttcaaggaa
     7681 agccagtaaa ggccgagctg gcgcacggct tgcatgaact cggcgaaatc gaccggcttg
     7741 cgcacatagc tgttgcagcc ccggctgtag ctctcgatga agtcccgctc ctcgcgcgac
     7801 gaggtgagta tgacgacggg caggaatcgc gtgcggtcgc cgccgcggat gcggtccagc
     7861 acctcgaacc catccactct gggcagattg aggtcgagca gaacgaccga aggcagaacg
     7921 tctgcgcccc ggccatcggg gccgaagagc aggtcgagcg cctcgacccc gtcgcgcgcc
     7981 acgacgatcc tggcgtcgac gtccatttgc cggaaggcga ggagcgcaag ttcctcgtcg
     8041 tccggattgt cctcgacaag aagaatggtc ttgtttttca cgatgcggcc tgatccaggg
     8101 aaaaccaaaa tcttgcgccc cggccgggct cggcctgggc ggcgatgtat ccgccatgac
     8161 gcttgacgat gcgctggacg gtggccaggc ctatcccggt accggggaac tccaccgcgc
     8221 gatgcaggcg ctggaaaggc gcgaacaacc tgcgcgcata acgcatgtcg aaacccgcgc
     8281 cgttgtcggc cacggcaaag cccggctcgc cgttctcgcc gggcatcgcg gcaaagctta
     8341 tccgggcatc aggcgtcttc gacgtgaatt tccaggcgtt gcccagcaga ttttccagcg
     8401 cgatgcgcag cagcctggca tcgccctcgg cagccaggcc gtcctcgatg gaaatgtcca
     8461 cccggcgctg cggctcggcc tggcgtaggt cggccaggac ttggcgcgcg atggcgctga
     8521 ggtcgacttt ctcccggtgc atttcttcgc gggaaacccg cgagagcttg agcaggtcgt
     8581 cgatcagctc gtccatcctg caggcagcca gctgcacgcg gccgagatag ccgcgcgcct
     8641 tctcgtcgag cgcgccggca tggttcttgg ccaggatctc ggtaaagccc gagatacccc
     8701 gcaagggcgc gcgcaggtcg tgcgacacgg aatggcagaa actctccaat tcatcgttga
     8761 gcgcccgcag ttcccgggtg cgatgggcga cgcgctgctc gagttcggca ttgaagacga
     8821 gcagccgggc ctgggccctt ttgtattcgg aaacgtcgtt gagaaagaac gtgcggactt
     8881 gcaggccgtc ctgctcgaac gccacggccc gcgcctcggc ttcgaagggc ccctgctccc
     8941 cgagcagcgt aaacgtgccc gcgccttgcg aagcctggat cgcgtcccaa tccgcggggc
     9001 cgtctggggc gcgtatcgcc tgagcgacgg gctggccgag cgctccggcc cttgccaggc
     9061 cgaactgttg ttccgcgacg gcgttccact cgatgatgac gcctcgggcg tcgagggtga
     9121 atatcgccgt attggtgtga tcgaagatag cggccttcag cgcgatcgag gccagttgct
     9181 gttccttggc gagcacctgc tggcgcactg cgacggacat gcggtccagc gccttgccca
     9241 gcagctgggt ttcccggaat gcgtccacga cggacaggcc gggctccggc gcttgcagcc
     9301 catgccgttc aatgcggtcg aggcgccgct ccagctcggc gagcggccgg ccgaaatggc
     9361 gggccatgcg cgccgaaagc agcgcgaggc ccgccacgac caccagcccc attccagcca
     9421 gttgatacag ctgccggcgc aaggatgctt ccatgagggg cgcatcgcgc gaagccacga
     9481 ccagccacgt gggcccgccc ggcagggtga cgcggcgggc ggcaacccag cggcgctcgc
     9541 cttccagcgc catgcgcgcc gtgaaaacgg cgtccgtgga tttgatcaac ggcgccaatt
     9601 cggcgttcag cggcagcggg gtccggccca cgccggcgcc gccgatcagg cgcggcgtct
     9661 ctcccagttc gacaacctgg agccgcgcat gatattcatc ggcgatggat ttcagaaaag
     9721 cattgaacgc cggcagatcg aaatccgtat cgagcacgct gacgagccgg ccctcctggt
     9781 cgcgcagcgc tctcacgtag ctgaagcccc agaggggctc gctgtgctcg tcatgaatga
     9841 tccacggata cgcgcgcatc catgtcccgc cgggcgcagc ggccagcgcc gcctggtaga
     9901 acggccgccc gcgcggatcg tagccatcgt aaggacgcga ttcgcgaagc acgaagcccc
     9961 ggtctgtcaa ggtgaagatg cgcgttaccg ggtcgttggc cagggtgccg ggaaacagcc
    10021 aaagcagtat ctcgcccttg gcggtccgct ccagggttcc gtattcgccc gcgtcgggca
    10081 ggatgatgcc gagatagctc agctccggcc gctgctcgaa cgcggccaag gcctgttcca
    10141 gggtggaacg caacgtcgcc cccgtcaccg ccgagaccag cgaggcgcgt accgccgaat
    10201 cggcggtcat ctccgccgcc cgggtgagca gctgcaggcg ctccgtcgag ctattgagcg
    10261 tttgggcgat gtcgcgccgc tcggctttct ccgtgctggc gatgccttgc cacgccagcg
    10321 cgcccagcag caccagcccg agcatcacca ccagccccac gacgttgccg atcagcgccc
    10381 gggagaacga catcggcagg acggccgtcg ctgcgcgggg atccgcatcg gaaagattgg
    10441 cctggggctg ggagttcacg ggaagctagc gcggctggtg ggtggattcg gctggtgaat
    10501 gctttcactg cgaaacatcc tcctcctgcc cctgacgccc ggcggcctcg cttgccgcca
    10561 tgcagcgcta cgactccccg caagaagagc acaacggatc aatcgtactt gatttttgta
    10621 agactttata aggaatggaa aataaaaccc gcataaatca acgacttatg cgggtttcgg
    10681 tgctgattgg gaccgggctc gccttgatgc gccattcaac gaacgcggca ccctgttgct
    10741 tccctcgaga cgggaagaaa acctctttgc gatcaatggt gtatttgggt ttggatcgaa
    10801 ttgttttttt gagcccaaac acgatgtgat gtggtgcggc cgacaggaat cgaacctgta
    10861 tcgagagctt cggaaactct aattctatcc attgaactac ggccgcagcg ggctgcattg
    10921 taccggactt atgcggccgg ctgggccagc agcggcaggc ccagctgcac gcggcgctgc
    10981 agccagaggc tggggcgata gcgcatgtcg ccggtgacgc gctgcatgtt caccaggatc
    11041 tccatgatgc ggccggcgcc cagggcatcg cccatggcca gcgggcccat gggatagccc
    11101 aggcccagcg tgacggcgcg gtcgatgtcc tcgggcgtgg cgatgcgctg ctgcgcgatg
    11161 tcgcaggcga tgttgacgat gtgcgccacg atgcgctgcg ccacgaagcc cgccgaatcg
    11221 gcgattacgc tcaccggcgc gccgtcgccg gccaggagcg cgtgggcggc gtcgcgccag
    11281 gacggcagcg tgcccggcga aagcatcagc gtgcggcggc ggccgggcgc gagttcgtgc
    11341 agcgtatcca ggcccaccgt gcggtcggcc tccaggccct gctgcgcgat gcaggtggac
    11401 acgtcctgcc catagggcgt gacgatgcac agcacgccgg cctcggactt ttcgctgagc
    11461 gtggcgccca ggcccgacag cagcgccgcg gcccgcgcgt ggccttgcga gctgtggcgg
    11521 ctcacccaga ccttcaggcc ggccggcagc gcgggcacgg ccggctcggc gggcacctgc
    11581 ttgcggccgt cgacataggc atagaagccc tcgcccgtct tgcggcccag caggccgccg
    11641 gcctggcgca ccgccgtgat cggcgacggg gtatagcgcg gctcgtcgta gaactgctgg
    11701 tagatggatt ccatcaccgg gtgcgagacg tccaggccgg tcaggtccag gagttcgaaa
    11761 ggacccatgc gaaagcccgc ctgctcgcgc atgatggcgt cgatctggtc gaagccggcc
    11821 acggattcct gcgccacgcg caggccctcg gtgttcatgc cgcggcccgc gtggttgacg
    11881 acgaagccgg gcatgtcctt ggcgcgcacg ggcgtgtggc ccatgcggcg cgccagcgcc
    11941 atcagcgcgt cgcccacggc cgggctgctg cgcaggccgt cgatcacctc gaccaccttc
    12001 atcagcggca cgggattgaa gaaatggtag cccgccacgc gcgccggctg cttgcaggcc
    12061 gcggcgatcg ccgtgatgga cagcgacgag gtgttcgacg ccaggatgca atcggccgcg
    12121 acgatggcct cgagcctggt gaacacctcg cgcttgatgt cgagccgctc gacgatggcc
    12181 tcgaccacgg cctggcaatc ggccagggcg tccaggccgg cggccggctc gacgcgggcc
    12241 agcgcggcct gggcatcggc ctcgctcagt ttgccctttt gcgccagctt gtcccaggtc
    12301 tggcgcagcg cttcgcgcgc ggcggccacg gcctcggcgc tggcatcgta aagccgcacg
    12361 cgcaggccgg cctgcgcggc gatctgcgcg atgccgcggc ccatggcgcc ggcgccgacg
    12421 atgccgatgg tttggatctc ttgcatggaa aatctctctt gtcggtaaaa ggggttcaat
    12481 ccgccggcct ggacagcgcc tcgatgtccg catcggacag gcccagccgt tcgcgcagca
    12541 cctcgtgcgt gtgctggccc agcatgggcg cgggccggtc gtagcgcacc gggctggccg
    12601 agagcttgag cgggctggcc gcgatgggcg ccacgccggc cgtggggtgc ggcagttcga
    12661 gcttcatgtt gcgcgccagc acctgcgggt cctggtagac ctgctccagc gtgttgatgg
    12721 ggccggccgg cacgccggcg gcctcgagtt cggccagcca ttggtcgcgc gcgccggtct
    12781 tcatgcgctc gaccaggatg ggcacgagcg cctcgcggtt gatcacgcgc tgcgggttgg
    12841 tggcaaagcg cgggtcctcg gccagttcgg tcatgccgat cacgcggcag taattgcgga
    12901 actggctgtc gttgcccacc gccacgatga ggtggccgtc ggcggccgcg aacacctggt
    12961 agggcacgag gttctggtgc gcattgccgg cgcgcctggg agccaccccg gatgtcatga
    13021 aattgaggtt ctggttggcc agcatggcca cctggcagtc gagcagcgcc atgtcgatgt
    13081 gctggcccag gccgctatgg ccgcgctcga tcagcgccgc caggatgccc accgtggaat
    13141 acatgccggt catcaggtcg gccacggcca cgccggcctt ttgcgggccg ccgccgggca
    13201 ggtcgtcgcg ctcgccggtg atgctcatca gcccgcccat gccctggatc atgaagtcgt
    13261 agccgggccg gctggcgtag gggccggtct ggccgaagcc ggtgaccgag caatagatca
    13321 ggcgcggatt gatctggcgc aggctttcat agtcgagccc gtatttgcgc aggccgcccg
    13381 ccttgaagtt ctcgacgaag atgtcgctct gcccggccag ttcgcgcacc acctcggcgc
    13441 cgcgcggcgt ggcgatgtcc accgccaccg acagcttgtt gcggttggcc gagaggtaat
    13501 aggccgcctc cgtggtgggg ttgccgtcct ggtccttcag gtagggggga ccccaggcgc
    13561 gggtgtcgtc gcccgccccc ggccgctcga tcttgacgac ctcggcgccc aggtcggcca
    13621 ggttctgggt acaccagggg cccgccagca cgcgggtcag atcgagaacg cgtatgccgg
    13681 tcaacggagc gggacgctgc ggcgcagatt gcacagacat gggttttaag cctacaagga
    13741 agcgacaagc cggtaatgtt agccaattcc ttccgaccgg ccacccataa agcctccgcc
    13801 catgacgatt tccactccca tcctgcgcga ccagctgccc gcgctgttca ccaagcgcga
    13861 atccgatacg aacaaaggca gcttcggcac cctgggcgtg ctgggcggcg gccccggcat
    13921 gacaggcgcg gccctgctgg ctgcgcgggc cgccttgcgc ctgggcgccg gcaaggtgct
    13981 ggtgggcttc gcccagggcg ccctgcccct ggcctgcgac ccgctgcagc tcgaactgat
    14041 gctgcgcacc ggccgcgacc tcatcaccga cgccggcgcg ctgggcgtga atgcctgggt
    14101 ggccggctgc ggaatcggcg cgacggccga cgcggcccgc ctgctggcgg cgctgttcca
    14161 ggcccgcaag aacgcgccgc tggtgctgga tgccgacggc ctgaacctgc tggcccaagg
    14221 cgccctgcag gccgactggg gtccggcccc cgtggtgctc acgccgcacc ccgccgaggc
    14281 cggacggctg ctggactgca cggccgccca ggtccaggcc gaccggcccg ccgcggcgca
    14341 agccctggcg cagcgctacc aggcgtggat cgtcctcaag ggcgcgggca ccctggtctg
    14401 cccgccggga ggaggcgact gcctggtcaa caccaccggc aacccgggcc tggccagcgc
    14461 cggtaccggc gacgtgctcg ccggcatgct gggcagcctg atcgcccagg gcgtgccgct
    14521 ggcccaggcc gtgcccggcg ccgtctggct gcacggcgcg gccgccgacg ccctggtggc
    14581 ggcaggcatc ggccccatcg gcctgacggc gggagaactg gcggaggctg cccgccggct
    14641 gcgcaacgcc ccctgaagcg tcacgaaatt ttcacaatgt ggcgaaaaca cccacaatac
    14701 ggaaattctt acaatatttt cgggtttacc ctagtgtttt tgttgcgctg cactacgggt
    14761 aatccctata ctggttccat acatcaccaa ggcactactg cctacctgga accaccatga
    14821 ataccaccaa cgaattccgc ctgaccgacg aactggaacg cgccctgctg cgtgaagcca
    14881 tggtcacccc ccgcgaagtc gaactgctgc ccgccctgaa gtcgggcctt gccgccatcg
    14941 gccgcgcttt cgacagcgtg ttcgccttca tcggcaacct gaacgacgcc atgaaccagg
    15001 cccgcgcccg cagcgcgacg tactccggtt cgcagtggta atgatgccgt aaggccctgc
    15061 gctcgcaggg tcccaggaca tgacgacggc cagtcggcgg gtttacccgc cgactggccg
    15121 ttttgctttt cagccccgaa ctccggggag ataatccccc gctacccacc cggcgtcccg
    15181 tccatggcgc cgcgcatcgg cagcgcgcca ccgctggcgg cgccgcgatg cctgccgcct
    15241 ggacgcatca cactgaattt ccctatcgat atgaaacccg ccgatctcag caacgccgac
    15301 cgcctggccc tggaactggg ccacgccggc cgcaacgact tcgcctatct cgacgaagac
    15361 cgcaacgccg accgccccat cacgctcaac acctaccgtc cctacggcta cacccccgac
    15421 cggccggtgg tgatcgtgca gcatggcgtc atgcgcaacg gcgacgacta ccgcgacttc
    15481 tggatccccg cggccgacca gcacaagctg ctgatcgtgg cccccacctt ttccgacgcg
    15541 gtctggcccg gcgtggaaag ctacaacaac ggcctggcct acaccgccat gggcaacccg
    15601 cgccacgtcg acggctgggc ctacgccctg cccggccgcg tggtggccga catggtcgcg
    15661 gccgaactca tcgacggcca gaacacctat ctcttcggcc actccgcggg cggccagttc
    15721 gtgcaccggc tgatgggcag ccaagcgcac gcgccctgga aggccgtggc catcggcaac
    15781 cccggctggt acaccctgcc cgacatggac cgcaagtttc ccgaaggcct gggcggcgtg
    15841 ggcctgaccg aggcgcacct ggagcgcctg ctggcctatc ccatgctgat cctcgcgggc
    15901 gaccaggaca tcgagaccgg cgggcacctg ccctccgacc cgccggccat ggcccagggc
    15961 ccgcaccgct acgcgcgggc gcactactat ctcgaggccg gccaggccga agcccggcgc
    16021 cgcggcattg cctgcaactg gtcgctgcgg agcgtgcccg gcatcggcca cgacggccgc
    16081 gccatgtcgg ccgcctgcgc cagcctgtgg ttcgaaggcc ggatgcccga tgccggcgcc
    16141 ctggccgcgc ttgccagcgg ccacgtcgcc tgaacccggg ccgtccgaac gccccaagca
    16201 cctagccgtt ccattcaacc acctcccctt tcggataccg ccatgagcac gctacccaat
    16261 accgagcagt tggtcgccgg catcattgcc tggatgcaat gcgaatctcc caccagttcc
    16321 ccggccggcg tggccgccat ggccgagatc accgccggct acgcccgcca gcaaggcctg
    16381 aaggccgccg tcaccccgct gggcgagaac gtcggcccca tgctctacgc caccaaccgc
    16441 gccgaaggcg acacccgccc cggcctgctc atcctggcgc acatggacac cgtgcacccc
    16501 gtcggcacgc tcgacgacaa ccccatccgc atcgagggcg accgcctcta cggccccggc
    16561 ggctacgaca tgaaggccgg cacctacatc gccctgtcgg ccatggccgg cctgggcgcg
    16621 cccggcgcca cggcgctgcc catcgacttc ctgatggtgc ccgacgagga aaccggcagc
    16681 cacgcctcgc gcccgcagat cgagaagttc gccgccggcg ccaaatactg tctggtgtgc
    16741 gagcctgcgc gggccgacac cggcatgtgc gtcaccgccc gcaagggcac cggcatgctg
    16801 cgcctgggcg tcaagggcat gccctcgcac gccggcgtgg cccaccagaa gggccgcagc
    16861 gccatcaagg aaatggccca ccaggtgctg gccctggaag ccatcaccga ctatgattgc
    16921 ggcatcaccg tgaccgtggg cacgattgcc ggcggcacgg ccaccaacac cgtgcccgag
    16981 cgctgccgct gcgtggtcga cttccgcgtg cccgacatgg cctcggccga ctacgtgctg
    17041 cgccgcatgc gcgaactgtg cgccgtcggc cccgacaccg agctggacat cgacgtcgag
    17101 ctgaaccgtc cgcccatggt aaagacggcc tccagcaccg cgctcctgga ccacgctcgc
    17161 gtgttcgcca aagaagccgg cttcgagctc aacgacgcgc ccatgaccgg cggcggcagc
    17221 gacgccaact tcacctcggc catgggcgtg cccacgctcg acggcctggg cgccgacggc
    17281 gacggcgcgc acacgctcaa ggaacacatc ctggtgtcca cgctggaaca gcgcgtgcgg
    17341 ttctggcact tgctgctcga gggactggcc tgaaccgggt acttgagact tgccatccca
    17401 ggcacatata attggacata tatgttccca tgggagggcc gaccatggcc cagatcaccc
    17461 tctacctcga cgaagacacg gaaatcctgg tcgcccaggc agccaaagcc aagggcatct
    17521 ccaagagccg gtgggtggcc gaagccatcc gccagtacgc tcacgatacc tggcccgcgg
    17581 aatgccttgc actggcaggc gcattccccg actttcccct gcgtgaagac tcgccatcct
    17641 ccggcgagga tctgcctcgc atcgggttct gaccatgctg gtactggaca gcaacaccat
    17701 cagctactac ttccggggcg atgaatgcgt cgtgccgcgc ctgcaggcac ttgccccgga
    17761 agaagtaggc gtgcccgcca tcgtggtcta tgaattgcgc tacggcctgc tgcgcttgcc
    17821 gtccgtggcg gcgcaacccc gccttgacgc tttggaggca ttgctgcgcc ccatgcaaac
    17881 cctggctttc gatgccgcat gcgccgagca ggctgccgcc ctgcgtcagt cgctcgaaaa
    17941 aaccggcacg cccataggcc cgcatgatgt gctgatcgcc gccaccgcgc tacggcacca
    18001 cgcccggctc gtcacgcgaa atgagcgcga gtttgcccgc gtgcccaacc tgcagatcat
    18061 caactggcat gccgcggcat gaccatccgg cacgcaaacc agcctgaacc gcaagtcagt
    18121 cttcctggct gcgcgcccag acacgcatcc ggtcttccaa ctgcgcgaga tcgatagccg
    18181 aagccaggta ggcatcgtgg cgggacgggc cgcgccacgc cagcaaacgg gacaagctgc
    18241 ggaaaaaaag cgaaaacatg gtctctaccc tgtccggcaa gcggacgggg acctcattga
    18301 agtcgaagga aaacgaaccc gcgcgtccag gcgcgcgaga cttacacggg cggcgtgtcg
    18361 cgaaacaccg gcagttgctc gcaacgcgcc gaaaacgttc gaagcgccgg gaaatcatcc
    18421 gccggcacga tcgccggcac catcatccgg gtgaaggtcc aagccaccgc gaccgccacg
    18481 cccgcgtgat ccaggacctc cgcatccagc gggtcggtgc gttgcgccgc ctccagcagt
    18541 ccgtaggccg ccagcaactg cccgcggaca cggtccagcc acggctggtg ctgtttttcc
    18601 ggaggccgca gattgtgctc gtagacgatc tgcatcgtct tttcgcaggc cgccagcgcc
    18661 aggcccgtca gccgcacggc gcgcgcccgc gccccgggct cggcggggaa gatgcttgcc
    18721 gccggcttgg ccaggctctg ggcatagtcg aggatgaggg tcgaatccat caacgcctgg
    18781 ccgtcatcca ggatcagcgt cggcgccttg accaccggat tcaaggcctg gaacgcctcg
    18841 aaggtgctga agacggacac cgcccgatgc tcgaacgcga tgccgagcaa tttgaggccg
    18901 attgccgtgc ggcggacata gggcgagtcg agcatgccga tgagttgcat ggcgcttccg
    18961 ttagaattgc ttaataaagt gcggcacccc gctcttccgg gccgcttctg taggcgccgg
    19021 caaaaaccaa gggcataccc taagattacc gactgccaaa tcgcaggaaa agcggtaaaa
    19081 atcaacactc aacgttagtt tttctaacat gaatcattta cccccgctgc cggcgctgcg
    19141 ttacttcgag gccgtggccc ggctcggcag cgtcacgtcg gccgcgcgcg aactgcatgt
    19201 cacgcactcg gccgtcagcc agcagatcaa gctgctggag gacatgatgg gcgtgccctt
    19261 gttcgtgcgc gcggcgcgcg gcctgacgct gaccgaagaa ggccgcctgt atgcgctcga
    19321 gatccgcgcc gcgctgcgcg atatcgaaac cgccacc
//
LOCUS       DASWPO010000208        21287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1297610, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000208 DASWPO010000000
VERSION     DASWPO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 21287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21287
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297610"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..999)
                     /locus_tag="VGC69_10860"
     CDS             complement(145..999)
                     /locus_tag="VGC69_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295820.1"
                     /translation="MKQPFNIASMMSSTARLLACGALLASSALAHATTLDEAKKALPD
                     AIKSSGTLRVATSLQWAPFAYSDENSQAAGIDIELVKLLAQKLGLKATFDDLKFPSIV
                     PGISTGRYDIGVDQIGITEEREKVVDMIPYFRSGFGLLVKKGSAPLNINKLCGHTLAL
                     TQGSSQVQIIGQMSTACTASGQKEIAQLFYPNSADTYMAVANGRGEGFIADHAVAVYI
                     AKLHGSLDVMPEVLDGKTLVAGIAVGKQLPTLKQALQVALQAAVQDGSYKALLTKYGV
                     PDSAYTEE"
     gene            1456..1722
                     /locus_tag="VGC69_10865"
     CDS             1456..1722
                     /locus_tag="VGC69_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295821.1"
                     /translation="MRAGRQGQRGQGMTEYIIVVALIAVAAIAVYQLFGQVVRSQTAA
                     MAKELAGEDGSSQSRQAGTAAATAAEQTKSRSLQSFTGNAKAAK"
     gene            1722..3083
                     /locus_tag="VGC69_10870"
     CDS             1722..3083
                     /locus_tag="VGC69_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY0295822.1"
                     /translation="MSRERLPYGGQALVPALLLLSVCGVAWAALYNLGQVAATRARLT
                     HAADAAAYSAALEQARALNLLAYINRAQIAHQVAMAHLVTLATWAQFSDAQARRRGMG
                     NPPAYLIGSLFGAKAEASYAAGRSTSGAPDEAIRAYRAHDDLVHDTLLHVARQAVSAL
                     SRQRADMVQLVLRANYPDLDDLPQALPWRVLADGWPGYVQFRPGAALQPLAYAAAGRY
                     DFLQPRNFTRTNSWMVEKRCPEKRHELRRRGATQMDEQGNWASFDSLSYHALRANKWI
                     GCYFREYPMGWGLAGDGGSGDTVQHAPEDFSAEDFWRWVSANTSWDIFSGSGNPLADA
                     YAQDDRATPHGRGLPGSDDVAAGREAEPLRFSIAVRQPAASLPISGGTSRLAWAGAYA
                     WRAFRPDEGVTVSAAAETYYVRPQARDDGKRELPTLFRPYWQARRVAVTTGEQSAAHG
                     AAR"
     gene            3080..3691
                     /locus_tag="VGC69_10875"
     CDS             3080..3691
                     /locus_tag="VGC69_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295823.1"
                     /translation="MTRQSGQGLVEALFALAALLACACAIAWTGSARHQAMQVAQASR
                     AAAFMAARGGTPPAGRDGVRLELSRDRAGSMPSDERQAQLVQDWLRLDPRLVTARAER
                     LVQPAGGWTFLSVGAVPVHRHTSVAQVGGHAESDMDGQRRILDSRIGWRQAADPSQSL
                     SQRLRQHLAGIDAAWGERKPDADWVSAWPGLVPAEKLTNGGRP"
     gene            3799..4392
                     /locus_tag="VGC69_10880"
     CDS             3799..4392
                     /locus_tag="VGC69_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295824.1"
                     /translation="MASGAKVYGAPAEVLSYEAPMDAPALIAFLAQQYPALRDMAVFP
                     GMAVLSDLSGPCSRVVTVSGQDGRGSRGTLSTICWEKAMTRPALPAPWLPAGAELVFD
                     FSEPAGRAGYVQQIWQYASPPDAVARQVHGNLARQGWRPAYDPDGAGSSWQTWQRGSE
                     TVIVDILGKAAGSMLAVLRFDSGQSTLAGQAASGGMP"
     gene            4389..5225
                     /gene="cpaB"
                     /locus_tag="VGC69_10885"
     CDS             4389..5225
                     /gene="cpaB"
                     /locus_tag="VGC69_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY0295825.1"
                     /translation="MKLTRWLPMARALGAYAFAAAAGLAAAWAVHEYIQQRTRAIEAQ
                     SRVPMVTRLVAARDLPAGASLDAESLAMRDVPQDWASSDSLPPEALGQIEGGMLALAV
                     RAGEPVLLHQVLRHKSEPVAQQLAAGRRAFSLPMQEIRDLPQHVQPDDRIDLYVSFAH
                     GGRELTVPLLQGGKVLSVDGEPGQPAHITLEAAASEAVKVVAARQGGVLTALLRPAAD
                     AGSREDASAPRDLPGLLGMAQPASRRRAVDIIYGDRIEGGDYGAFADDAAAAPAARRG
                     AP"
     gene            5222..6658
                     /locus_tag="VGC69_10890"
     CDS             5222..6658
                     /locus_tag="VGC69_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEY0295826.1"
                     /translation="MTTLRRAGKGFAPLDMLRRNLVGLFILCGPAYGAQDTPQSLELD
                     LEVGESRVLSMPGARRVAVGNGQVLSAIADDEREVVLFARQEGMSSVHIWSGSPVPAA
                     YAVRVLPAGLRRMRAEVDKLLEGIPAAHSTQVGGNLVIEGNDLSDEDRARIAALAQRY
                     PGILDFTGQVGWDRMVLLDVQVVELPRSRLTDFGVRWDGATQGGLQAGMVWDGGTAAG
                     MLSERPGTAPLAAALPTYRAASYFGANAVLSSSLAALAQRGEAVMLAQPQLLARSGST
                     ASFLAGGEVPYTSSDRNGVPTTLFKPYGVSLHITPRIDRNGAIRSRIEVEASSVDASM
                     SLPSGPALRTRRAVTEFNVRSGQTLVLGGFLSREQSFERSGIPLLSEIPVLGSLFGAD
                     KTQRRDTELAIFVTPSIVSNDYAGFAQSVAQGRQVLQQAFPEPTQLRLLPPAGATRGW
                     SAPEGAGSQWQSGNGDEPDRLATPGKEP"
     gene            6661..8229
                     /locus_tag="VGC69_10895"
     CDS             6661..8229
                     /locus_tag="VGC69_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0295827.1"
                     /translation="MLEITLIFEDGTRQVHAAMPPVSIGRAPQCAVRIANWRVGRQHA
                     RLVRQGGDILIEDLGTLGGTLVNGERVATHGPLTAEDRILIGPCQIQVRERQETPPPS
                     DPVSNNEAAPPASAASMETPAALAWLPHRRSLHAALLQALDLRRRDVSGMSDQALREE
                     ARRLLDELVQEADFPAQLDRALLCRMVLDEAVGLGPLESLLADPGITEIMVNRHDEVY
                     VERAGRLSRHDTVFSSEQAVRGVIDRIVAPIGRRIDESSPMVDARLPDGSRVNAVIAP
                     IALRGASLTIRKFPLRRPQMPDLVRLGSLNEAMAELLALCVRRRKSIVVSGGTGSGKT
                     TLLNILSNAIPDGERIVTIEDAAELRLGHAHLVALEARPPNLEGRGRIDIRDLVRNAL
                     RMRPDRIVVGECRGAEAFDMLTAMNTGHEGSLTTLHANSPRDALSRLEAMTLMAGLDL
                     PLAVVREHIASSVDIVVQQVRLADGRRVVTGIVEVTGMESGRIQLQDLFHYEHGHGFV
                     DSGMRPTFLEGPAP"
     gene            8226..9071
                     /locus_tag="VGC69_10900"
     CDS             8226..9071
                     /locus_tag="VGC69_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0295828.1"
                     /translation="MIWLAFVLTGLSVAALAWSAQAWVGPALRRYRQVYTQETGVKLS
                     ELFLFVDPAQLWLASLACAALGGLSAWALSGSGLAGVLAGLLATRLPGVLVETLRRRR
                     LLRFERQLPVALMSLAGGLSAGASLAVALKHIVEQSEAPLSQEFGLMLREQRLGVPFA
                     AALDHLHERVPSEATVLVTAALRVAAQTGGNLAEALERIADTLRSRLLLQGKIRALTA
                     QGRMQAWIVGSLPVLLALVLNRLEPQAMAVLWSTPMGWTVLAVVLLLETTGVMMIRRI
                     VNIDI"
     gene            9081..9944
                     /locus_tag="VGC69_10905"
     CDS             9081..9944
                     /locus_tag="VGC69_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0295829.1"
                     /translation="MSVLAIMLSGLCAAGLVYLLLAPLRPGLQPVPAVSSMPWWWRCA
                     WPWTRALTPLAEPLMSWRTREKKRQALGRAGVPPQIGPQHWLAMTWLMACAGGGAALG
                     LALVLAWPPAIPVALAALAGAGWPGLWLRSQAMRRKRRIDRDMPFVLDMMTLCVEAGL
                     GLHGALQQSVQCSAPGPLRDTLDGALADMRTGMSRMAALKAMAGRSGSASLQAWAASM
                     AQADALGMSLGPLMRGQAAQCRADRFQHAERLAMEAPVKMLLPLIGCIFPCTFIVLGF
                     PIAWQLLPALQ"
     gene            complement(10190..10996)
                     /locus_tag="VGC69_10910"
     CDS             complement(10190..10996)
                     /locus_tag="VGC69_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEY0295830.1"
                     /translation="MQAASLPATSGWQWIRAGLQLFRKQPLAMFTWAMVISLFVVFAA
                     LTLPIGALLLPLLMPVITVMTLAACKHVEAGRIMLPSMWPKPLLRPGVFRKLMLMGLL
                     YTAFCIAAELVAVLPFADDVMDGMSVASTAQDLAPLMMAMRKPMLVFVALYVPIAALF
                     WHAPVLVAWHGVRLMQSLFFSIVACWRCKWAFLVYGVAWALIFMFIDLIAGLLVTAGM
                     PGTLVDSLQVPVNIIAGGVLYCSFYPAYTSVFGIDDDTRTGLDHPDSAHA"
     gene            complement(11061..12023)
                     /locus_tag="VGC69_10915"
     CDS             complement(11061..12023)
                     /locus_tag="VGC69_10915"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY0295831.1"
                     /translation="MAVFTPVSDDDARALLARYNLGTLVSLRGITSGIENTNYFLGTS
                     RGEYVLTLFEVLTREQLPFYIELMHHLAQRGIPVPEPQTLDDGTRLTELNGKPCAIVT
                     RLPGSYEPAPGKAHCGLAGATLARAHLAGRDFAIRQPNLRGLDWWRQTAPKVRPFLNP
                     AQTELLDAEIAAQVEACAAWPALPAGPAHCDLFRDNALFAGTHDSPRMGGFIDFYFAG
                     CDTWLFDVAVCLNDWCIVHETGEFIPELAQAWLAAYAAVRPFTDAERQAWPLMLRAAA
                     LRFWLSRLYDFFLPRPAQTLKHNDPTHFERVLLARHRLAPPALP"
     gene            complement(12119..13408)
                     /gene="egtB"
                     /locus_tag="VGC69_10920"
     CDS             complement(12119..13408)
                     /gene="egtB"
                     /locus_tag="VGC69_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY0295832.1"
                     /translation="MPAATPAPASAAQRYDAVRQLSLLLAQPLSPEDCQAQSMPCCSP
                     VKWHLAHTTWFFETFVVQRADPGRPPYDPRLRQLFHAYEVGDSKLPPASHGLLTRPSL
                     AEVLDYRAHVDATMHALLSRPGGLGDQAGAFFDLALQHEQQHQERQLADIKHLLSCNP
                     LRPAYAQAPAPGALPAGTPAGWTHYAGGQVRLGHEGAGFAFSNEHPAHGVLLPPYYLA
                     DRLVTQGEYLEFMLDGGYRRPEIWLSLGWEAVQSAGWKAPLYWEGQKDAWQVYTLHGM
                     QALDPHAPVVHVSYFEADAYARWARVRLPREAEWEHAARLRKEGNGDRPNLLENGLLQ
                     PRPAPVPMLARNGHGPVQLLGDAWEWTSSAYDAYPGHQPPAGMQGEYNGKFMCNQYVL
                     RGGSCITPGSLIRTTYRHFLPPGARWQFTGIRLARNA"
     gene            13786..14145
                     /locus_tag="VGC69_10925"
     CDS             13786..14145
                     /locus_tag="VGC69_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295833.1"
                     /translation="MARSAAHALVADGFSEDVVSVFYGRRPARSRGLWARLVEAVRRR
                     RRSAGEILLVVQLSSADVQAAIGLLRDAGSVSVEQSQDDWQTGPWERAAVHRIRPVAR
                     RPAGPGPVAHRAQWQQL"
     gene            complement(14158..15816)
                     /locus_tag="VGC69_10930"
     CDS             complement(14158..15816)
                     /locus_tag="VGC69_10930"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY0295834.1"
                     /translation="MRWIVNRYTTFALILIGTIVTALLAVRYTAYWLWAVIPLGALSL
                     LGIYDMLQTRHAIRRNYPVLGNLRFLFEFIRPEIRQYFLEDDTNASPFSRAQRSIVYQ
                     RAKEELDKRPFGTQEDVYSDRYEWINHSLAPAHIEDTDFRIMVGGPDCKQPYAMSVFN
                     ISAMSFGALSANAVLALNEGARLGNFAHDTGEGGISRYHRKAGGSLVWNIGSGYFGCR
                     DEQGRFSEEAFVRNACTPQVKLIEIKLSQGAKPGHGGILPAGKVTIEIAEARGVPAWQ
                     DCNSPAAHSAFDSPIGLLHFVARLRKLSQGKPVGFKFCMGHPWEWFAIVKAMLQTGIT
                     PDFIVVDGAEGGTGAAPVEFVDHVGTPLREALRLVHNTLVGANLRDRIKLGASGKIIT
                     AFDMARVLAMGADWCNAARGFMFALGCIQAQACHTGKCPTGVTAQDPLRQRALVVPDK
                     AQRVHNFHRNTLHALAELLCAAGLTHPGDLRPQHIARRISPAEVRLLSALFPTLSPGE
                     LLRGEFRHDVFRIGWHMAQADSFQPACSINDVPLAQPRQQPVAA"
     gene            complement(15943..17169)
                     /locus_tag="VGC69_10935"
     CDS             complement(15943..17169)
                     /locus_tag="VGC69_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY0295835.1"
                     /translation="MSTHASALPLPASLPPIFANRKPLLVALLIWLAGAAYLLQTVSW
                     RQSALWIVGGLLGVTLYHAAFGFTQAWRVFVSDRRGAGLRAQMVMLAFGVLLFFPFLS
                     QGSLFGQPVVGLVSPAGVSVLLGAFLFGLGMQLGGGCASGTLFAVGGGNTRMIVTLLF
                     FIVGSVLATYTYGWWSALPALPPTSLVKSWGVPTALVANLVVFALIAWITLVLEKRRH
                     GHVISTTTGTNRPPSLLRGPWPLVAGGVVLVLLNFATLALAGRPWGITSAFALWGVKV
                     LQAFGTNIGGWQFWASQKAALADPVSHDVTTVMDIGIMVGALAAASAAGKFAPVWKVP
                     ARSLVGAVVGGLLLGFGSRLAYGCNIGAYFSGILSGSLHGWLWLPAAFVGSALGVTLR
                     PLFGLAVEKTPTPSSC"
     gene            17325..17942
                     /locus_tag="VGC69_10940"
     CDS             17325..17942
                     /locus_tag="VGC69_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0295836.1"
                     /translation="MTEIPLFPLGSTLFPAGVLHLRIFEVRYLDMVKRCIAEGSSFGV
                     VALYAGGEVRAPHGGEVLSPAGTLACITQWEAPAPTLYQLTCTGSTRFRLLRSWQAKY
                     GLWMGEVAWLPDDPVEEIPAELQPSADALGRLIAGMQQQGVPSAQMPLAPPFRLDECA
                     WVANRWCELLPLPATEQVHLLELSDPVERLRAVHALLSGMLPPQA"
     gene            complement(17964..18992)
                     /locus_tag="VGC69_10945"
     CDS             complement(17964..18992)
                     /locus_tag="VGC69_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0295837.1"
                     /translation="MQAADLNVLLACQGWLRQGRKAVLLTVLRTWGSSPRPPGAMMAI
                     RDDAVVSGSVSGGCIEDDLIDRVRQTGIASLCPGNRPAVAIYGIQAEEARRFGIPCGG
                     TLQLVLEPLAAHSRIDDTVRILQQRQALRRDIDMRTGAATLGCASAEVPPQLQEEHFS
                     YTLGPSNRLIVIGAGQLSRYLCEIATGLDFDVTVCDPREEYRADWNIPGVHLTYDMPD
                     DVVIAAQPDPRTAVVALTHDPKLDDLALMHALQSEAFYVGALGSRANNARRRERLRQY
                     FDITEAQLARLHGPAGLYIGSRTPPEIALSILAEIVAYKNGVNLPDSLAVGTAKETIE
                     HLPRRNAA"
     gene            complement(19008..19388)
                     /locus_tag="VGC69_10950"
     CDS             complement(19008..19388)
                     /locus_tag="VGC69_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="HEY0295838.1"
                     /translation="MVSRLLNALMPLALTALLAGCAAPGSNGSSAAGATGGTGSYSSA
                     SYSGSASASGPDAPSKDCDAQPVQNMLGQAYGDSVGESVRQRSGSNAVRLLKPGQVMT
                     MEYNPARINIILDAKGAIEALRCG"
     gene            complement(19448..20803)
                     /gene="gorA"
                     /locus_tag="VGC69_10955"
     CDS             complement(19448..20803)
                     /gene="gorA"
                     /locus_tag="VGC69_10955"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY0295839.1"
                     /translation="MNYDYDLYVIGAGSGGVRASRFAASFGARAAIAESRYLGGTCVN
                     VGCVPKKLLVYGAHYAEDFEQAAGFGWTAEAPRFDWPRLIANKNREIQRLNDIYRSLL
                     VNAGVTLHEGHARIVDPHTIEINDQRHTAKHILVATGSWPFVPEIPGREHITTSNEAF
                     FLETLPRRVVVVGGGYIAVEFASIFNGMGAQVTQLYRGALFLRGFDNGVREHLREELA
                     KKGVNLRFNADPARIDKLPDGTLRVTLQDGAALDADCVFYATGRRAMVDNLGLENTRV
                     QFDKDGFIEVDGDYRTADPSILALGDVINRVPLTPVALAEGMAIARQLFRPEEYQRVD
                     YSLIPTAVFSLPNIGTVGLTAEQARATGHTVKIFESRFRPMKLTLTDSQEKTLMKLVV
                     DAETDRVLGCHMVGPDAGEIIQGLAVALKAGATKRIFDETIGIHPTAAEEFVTLRTPV
                     A"
     gene            21013..>21287
                     /locus_tag="VGC69_10960"
     CDS             21013..>21287
                     /locus_tag="VGC69_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0295840.1"
                     /translation="MSAVRTNEYGLPIGPELPGWSARARPPCTPIQGRFCRIEPLAPA
                     RHADDLVAAFRQAPDGRAWTYLAVGPFTDPAEHRAWLAKAAAGEDLL"
BASE COUNT         3357 a         6583 c         7736 g         3611 t
ORIGIN      
        1 cgatctccca ctggaagccc gggttcgtcc cgatctggta gaccacccag gccacgaaga
       61 ccagggccag gacgatgcag accccccgcc cgggccgggc gcgctcggcg atcttgattt
      121 cgacgtctgt catgccgggc ccgcttactc ttcggtgtag gcgctgtcgg gcacgccgta
      181 tttcgtcagc agcgccttgt agctgccgtc ctgcaccgcc gcctgcagcg ccacttgcag
      241 ggcctgcttc agggtgggca gctgcttgcc cacggcgatg cccgccacca gcgtcttgcc
      301 gtccagaact tccggcatga catccaggct tccatgcagc ttggcgatat agacggcaac
      361 ggcgtgatcg gcgataaagc cctcgccgcg gccattggcc acggccatat aggtgtcggc
      421 gctgttcggg tagaagagct gcgcgatttc tttctggccg ctggccgtgc aggccgtcga
      481 catctgtccg atgatctgga cctgggaact gccttgggtc agcgcgagcg tgtggccgca
      541 cagcttattg atattcagcg gggcgctgcc cttcttgacc agcaggccga atcccgaacg
      601 gaaataaggg atcatgtcga cgactttttc gcgctcttcg gtgatgccga tctggtcgac
      661 gccgatatcg tagcggcccg tcgaaatgcc gggcacgatg gacggaaact taaggtcgtc
      721 gaaggtcgcc ttcagaccca gcttctgggc cagcagcttg accaactcga tatcgatgcc
      781 cgctgcctgg ctgttttcgt cggaataggc aaacggagcc cattgcaggg aagtcgccac
      841 gcgcaaggtg ccgctgctct tgatggcgtc cggcagcgct ttcttggctt cgtccagcgt
      901 cgtggcatgg gccagcgcgg aactggccag cagcgcgccg caggcgagca atcgcgctgt
      961 cgacgacatc atcgacgcga tgttgaaagg ctgtttcatc ttttccctgt tttgttcggt
     1021 gcgtttgttc ggtgcgaaat taatgctctg cttgagcatg aatagagtgt aggcactata
     1081 aattgccaag taaatattat ttctgccgtc tacgtgaaat ccctgatgtc gaccgttgcg
     1141 cgaggccccg gggtgatccg cttgagtgct gcctatcgag ctgaattacg ctgaaatggc
     1201 ttgatttata gggtcggctg catgtatgga cgatcgatgg ccaagatggg ccgcgattca
     1261 tgcagcggac ttacaattca aggccgtgaa agagcagatt gagcgtacac acttatgttt
     1321 ttgtgtgcgg cacgcatcat cgagggcctg tcaatgcatc gggacgcttt gatccacctc
     1381 ggcatccgcg catagccgcg caggactggc gtatccatac tttcccccac gttcaaggct
     1441 tcagggggaa gcatcatgag ggcaggcagg caaggtcagc gcgggcaggg catgacggaa
     1501 tacatcatcg tggtggcgct catcgcggtg gccgcgattg cggtgtatca actgtttggg
     1561 caggtcgtgc gttcgcagac ggcggccatg gccaaggaac tggccgggga ggatggttca
     1621 tcgcaatccc ggcaggctgg tacggcggcc gccacggcgg ctgaacaaac caaatcgcgg
     1681 tccctgcagt cgttcacggg caatgccaag gcggccaagt aatgtcccgc gaacggctgc
     1741 catatggcgg acaggccctg gtcccggcct tgctgctgct gtcggtctgt ggcgtggcct
     1801 gggcggcctt gtacaacctg ggccaagtgg cggcgacccg cgcccgcctg acccacgccg
     1861 ccgatgccgc ggcatacagt gccgccctgg agcaggcccg cgcgctgaac ttgctggcct
     1921 atatcaaccg cgcgcagatc gcgcaccagg tggccatggc ccacctcgtc actttggcga
     1981 cctgggcgca gttttccgat gcccaggctc ggcggcgcgg catggggaat cctccggcct
     2041 atctgatcgg ctccttgttc ggcgcaaagg ccgaggcatc ctatgcggca gggagatcca
     2101 cctcgggtgc gccggacgag gcaatccggg cgtaccgggc gcacgacgac ctggtgcacg
     2161 acaccttgct gcacgtggcg cggcaggctg tttcggcctt gtccaggcag agggcggaca
     2221 tggtccagct tgtcttgcgc gccaattatc ccgatctgga tgatctgccg caggccttgc
     2281 catggcgcgt gcttgccgat ggctggccag gctatgtgca atttcgtcca ggggcggcgc
     2341 tgcaacccct ggcctacgct gccgcggggc gctacgactt cctgcagccc cggaatttca
     2401 cccgcaccaa tagctggatg gtcgagaagc gttgccccga aaagcggcat gagttgcgcc
     2461 ggcgtggcgc aacgcagatg gatgagcaag gcaactgggc ctcgttcgac tccttgtcgt
     2521 atcacgccct gcgggccaat aaatggatag gctgctattt ccgcgaatac cccatgggct
     2581 gggggctggc gggagacggc ggctcgggcg ataccgtgca gcatgctccc gaggattttt
     2641 ccgcggaaga tttctggcgt tgggtatcgg ccaatacctc gtgggacatt ttttcgggca
     2701 gcggcaatcc cttggccgat gcctacgccc aggatgaccg cgctacgccg cacgggcgag
     2761 gactgccggg cagcgacgac gtggcagcgg gccgggaggc cgagccgctg cgcttttcga
     2821 ttgccgtccg gcaaccggcg gcgagcctgc ccatctcggg cggcacaagc cggcttgcct
     2881 gggctggcgc ttatgcctgg cgggccttcc ggccggatga aggcgtcacg gtcagcgccg
     2941 cggccgaaac ctattatgtg cggccccaag cccgcgatga cggcaagcgt gagctgccta
     3001 ccctgtttcg tccttattgg caggcgcgtc gcgtcgccgt gacgaccggc gagcagtccg
     3061 cggcccatgg agcagcaaga tgacgaggca gagcggccag ggattggtcg aagcgctttt
     3121 tgccctggct gccttgctgg cttgcgcctg cgccattgcc tggaccggtt ccgcacggca
     3181 ccaggcgatg caggtggcgc aggcgagccg tgcggcggcc ttcatggccg cgcgcggcgg
     3241 taccccgccg gccggtcgcg acggcgtgcg gctcgagctg agccgggatc gggcgggatc
     3301 gatgccgtcg gatgagcggc aggcgcaact ggtgcaggac tggctgcgtc tcgatccccg
     3361 gctcgtgacg gcaagggccg aacgcctggt acagcccgcc gggggttgga cgtttttatc
     3421 tgtcggggcc gtgcctgtcc accgccatac ctcggttgcg caggtgggtg gacatgccga
     3481 atcggacatg gatgggcagc ggcgcattct ggacagtcga atcggctggc gccaggccgc
     3541 ggatccgtcg caatccctgt cgcagcgctt gcggcaacac ctcgcaggca tcgacgcggc
     3601 ctggggcgag cgcaagccgg atgccgactg ggtatcggca tggccgggcc tggtgcccgc
     3661 cgaaaaactg accaatgggg ggcggccgtg attgcgcgtc gtcatcgctt atggcttgcc
     3721 gcggcttgtc tggcccccgc ggctgccggc catgccgcag cctcgtgggt attgcccaag
     3781 ggtagcgcga tggtcgaaat ggcatcaggc gcaaaggtgt acggcgcgcc agccgaggtg
     3841 ctgtcgtacg aggccccgat ggacgcgccg gcgttgatcg ccttcctggc gcagcaatat
     3901 ccggctttgc gagatatggc ggtgttcccg ggcatggccg tgctatcgga tctcagcggg
     3961 ccctgttccc gcgtggtgac cgtttcgggg caggacggac gcggcagcag gggaaccctg
     4021 tccaccattt gctgggagaa ggccatgacc cggcccgcat tgcctgcgcc ttggctgcct
     4081 gccggcgcgg aactggtgtt cgacttttcc gagccggcgg gccgggcggg atatgtccag
     4141 cagatatggc aatacgcgtc gccgcccgat gccgtcgcca ggcaggtgca cggcaatctt
     4201 gcgcgccagg ggtggcggcc cgcatacgac ccggatggcg ccgggtcttc gtggcaaacc
     4261 tggcagcgtg gatcggagac cgtgatcgtc gatatcctgg gcaaggccgc gggcagcatg
     4321 ctggctgttc tgcgattcga ctccggccag tccacgctgg cagggcaggc cgcatcggga
     4381 ggcatgcctt gaagctgacc cgatggttgc ccatggcgcg tgcgctgggg gcctatgcat
     4441 ttgccgcggc ggcagggctg gcggcggcct gggccgtgca tgaatacata caacagcgca
     4501 cgcgggccat cgaagcccag tctcgcgtac ccatggtgac caggctggtc gcagcccggg
     4561 atctgccggc aggcgcatcg ctggacgccg aaagcctggc catgcgggac gtgccccagg
     4621 actgggcatc atccgattcc ttgccgcccg aagcgctcgg ccagatcgag ggcggcatgc
     4681 tggcattggc cgtcagggcc ggggaacccg tcctgctcca tcaggtcttg cggcacaaga
     4741 gcgagcccgt cgcgcagcag ctggccgccg gccgccgggc cttcagcctg cccatgcagg
     4801 aaatccgcga tcttccccag catgtgcagc ccgacgaccg catcgacctg tacgtgtcgt
     4861 ttgcgcatgg cggacgcgag ctcacggtcc ccttgctgca aggaggaaag gtgttgtccg
     4921 tcgatggcga gccggggcag cctgcccata tcacgctgga ggccgccgcc agcgaggcgg
     4981 tcaaggtcgt tgccgcgcgg cagggcggcg tgctgacggc cctgctgcgt ccggccgccg
     5041 acgcgggatc ccgcgaggat gcctcggcgc cgcgcgatct tcctggcttg ctcggcatgg
     5101 cgcagcccgc ctcccgccgg cgagccgtgg acatcatcta tggggaccgg atcgaaggcg
     5161 gggactatgg tgcttttgcc gacgatgccg ccgcggcgcc ggctgccagg cgaggtgcgc
     5221 catgacgacc ttgcgccgtg ctggcaaggg tttcgcgccg ctggacatgc tgcggcggaa
     5281 cctggtcggc ctgttcatat tgtgcggccc ggcttatggc gcacaggaca cgccccaaag
     5341 cctcgaactc gacctggagg tcggcgaaag ccgggtattg tccatgccgg gcgcccgccg
     5401 ggttgccgtg ggcaacgggc aggtgctcag cgccatcgcc gacgacgagc gggaagtcgt
     5461 gctgtttgcc cggcaggagg ggatgtcctc ggtgcatatc tggtccggtt cgccagtgcc
     5521 cgcggcctat gccgtgcggg tattgcccgc gggcctgcgc cgcatgcgcg ccgaggtcga
     5581 caaattgctc gagggcatcc cggcggcgca tagtacccag gtgggcggca acctggtcat
     5641 cgagggcaat gatctgtccg acgaggaccg ggctcgcatc gccgcgctgg cccagcgcta
     5701 tccgggcatc ctggatttca cgggacaggt gggttgggac cgcatggtgc tgctcgatgt
     5761 gcaagtggtc gagctgccgc gttcccgcct gaccgatttc ggcgtgcgct gggatggcgc
     5821 cacgcagggc ggcctgcagg ccggcatggt ctgggacggc ggtacggcgg cgggcatgct
     5881 gagcgagcgt cccggaaccg ctccactggc cgcggccttg cctacttacc gtgccgccag
     5941 ctatttcggc gccaacgccg tgttgtcttc cagcctggct gccctggcgc agcgcggcga
     6001 ggcagtgatg ctggcgcagc cgcaattatt ggcccgcagc ggcagtacgg cttccttcct
     6061 ggctggcggc gaagtgcctt atacctcgtc cgaccgcaat ggcgttccca ccacgctgtt
     6121 caaaccttat ggcgtgtcct tgcatatcac gccgcgcatc gatcgcaacg gggcgattcg
     6181 ctcccgtatc gaggtggagg ccagctccgt ggacgcctcc atgagcctgc cgtcggggcc
     6241 ggcgctgcgc acgcggcggg cggttaccga attcaatgtc cgttcgggcc agaccttggt
     6301 gctgggtggg ttcctctcgc gcgaacaatc gttcgaacgc agcgggattc cgttgctgtc
     6361 cgagattccc gtgctgggca gcctgttcgg cgcggacaag acccaacggc gcgataccga
     6421 gctggccatt ttcgtgacgc ccagcatcgt gtcgaacgac tacgccgggt ttgcgcaaag
     6481 cgtggcgcaa ggacgccagg tgctgcagca ggccttcccc gaacccaccc aactgcgcct
     6541 gttgccgccg gcgggcgcca cgagaggctg gagcgcgccc gaaggcgccg gctcgcaatg
     6601 gcaatccggc aacggcgatg agcccgaccg tttggccaca ccaggcaagg agccctgaac
     6661 atgctggaaa tcacactgat attcgaagac ggcacacggc aggtgcatgc cgccatgcca
     6721 cccgtgtcaa ttggccgcgc gccgcaatgc gccgtgcgca tcgccaactg gcgggtaggc
     6781 cggcagcacg ccaggctggt gcgccagggc ggagacatcc tgatcgaaga cctgggtacc
     6841 ttgggcggca ccctggtcaa tggcgaacgc gtggcgacgc acggcccttt gacggcggag
     6901 gatcgcattc tcatcgggcc ttgccagatc caggtgcgtg agaggcagga gacgccgccg
     6961 ccatcggatc cggtttccaa caatgaggcc gcgccgccgg caagcgccgc ctccatggaa
     7021 acgccggcgg ccttggcctg gctgccgcac cggcgcagcc tgcacgccgc gctgctgcag
     7081 gccctggatc tgcgtcggcg ggatgtgtcg ggcatgagcg accaggcctt gcgcgaggag
     7141 gcccggcggc tgctggatga actggtccag gaagcggatt ttccggccca gctcgatcgg
     7201 gcgctgcttt gccgaatggt gctggatgag gcggtcgggc tcggtccgct cgaaagcctg
     7261 ctggccgatc caggcatcac cgaaatcatg gtgaaccgcc atgacgaagt ctatgtggaa
     7321 cgcgcgggac ggctgtcgcg gcacgatacg gtgttcagca gcgaacaggc cgtgcgcggc
     7381 gtcatcgacc gcatcgtcgc cccgataggc cggcgcatcg atgaaagttc gcccatggtg
     7441 gatgcgcgcc tgcccgacgg ctcgcgcgtg aatgccgtga ttgcgcccat tgccctgcgc
     7501 ggcgccagcc tcaccatacg caaattcccg ctgcggcggc cgcagatgcc cgatctggtc
     7561 cgcctgggca gcttgaacga ggcgatggcc gaactgcttg ccttgtgcgt gcgccgccgc
     7621 aagagcatcg tcgtgtccgg cggcacgggt tcgggcaaga ctaccttgct caacatcctc
     7681 tccaatgcca ttcccgacgg cgagcgcatc gtcacgatcg aggacgccgc cgagctgcgg
     7741 ctggggcatg cgcacctggt tgcgctggag gcgaggccgc ccaatctgga aggccggggc
     7801 cgcatcgata ttcgcgacct ggtgcgcaat gccctgcgca tgcggcccga tcgcatcgtg
     7861 gtgggggagt gccgaggcgc ggaagccttc gacatgctga cggccatgaa taccgggcat
     7921 gaaggttcct tgaccaccct gcatgccaat tcgccgcgcg atgccttgtc gagactggag
     7981 gccatgacct tgatggccgg gctggacctg ccgctggccg tcgtgcgcga gcacatcgcc
     8041 tccagcgtgg acatcgtggt gcagcaggtc cggcttgcgg acggcaggcg ggtggtgacg
     8101 ggcatcgtcg aagtcacggg catggaaagc ggccggatac agctgcagga cctgttccat
     8161 tacgaacacg ggcacgggtt cgtcgattcc ggcatgcgtc cgacttttct ggaaggaccg
     8221 gcgccatgat ctggctggct ttcgtgctga cgggcttgag cgtcgccgcc ctggcctgga
     8281 gcgcccaggc atgggtcggt ccggcattgc ggcgttatcg gcaggtctat acgcaggaaa
     8341 ccggagtcaa gttgagcgaa ctgttcctgt tcgtcgaccc ggcccagttg tggctggcct
     8401 ccttggcctg cgcggccttg ggcgggctgt cggcatgggc gctgtcgggc agcgggctgg
     8461 cgggtgtgct ggcgggattg ctggccacgc gattgccggg cgttctggtc gagacgttgc
     8521 gcaggcgcag gctgctgcgg ttcgagcgcc aattgccggt ggcgctgatg tcgctggcgg
     8581 gcggattgtc ggctggcgcg agcctggcgg tcgcgctcaa gcatatcgtc gaacaaagcg
     8641 aggcgccgtt gtcgcaggag ttcggcctga tgctgcgcga gcagcgcctg ggcgtgcctt
     8701 ttgccgccgc gctggatcac ctgcacgagc gcgtgccatc ggaagccacc gtcctggtga
     8761 cggcggcctt gcgcgtggcg gcgcagacgg gcggcaacct ggccgaagcg ctcgagcgta
     8821 ttgccgatac cttgcggtcg aggctgctcc tgcaaggcaa gatccgcgcc ttgacggcac
     8881 agggccgcat gcaggcctgg atagtcgggt ccttgcccgt gttgttggcc ctggtgctga
     8941 accgtctcga acctcaggcc atggcggtgt tgtggagcac gcccatgggc tggacggtgc
     9001 tggctgtcgt cctgctcctg gaaacgacgg gcgtgatgat gatccggcgc atcgtgaaca
     9061 tcgacatcta ggagtgtggg atgagcgtcc tggccatcat gctgtccgga ctgtgcgcgg
     9121 cggggctggt ttacctgttg cttgcgccgc tgcgcccagg gctgcagccc gtgccggccg
     9181 tatcgagcat gccctggtgg tggcgctgtg cctggccatg gacgcgcgcg ctgacgccgc
     9241 tggcggaacc gctgatgtcg tggcggacgc gtgaaaaaaa gcgccaggcc ctgggccgcg
     9301 ccggtgtgcc gccgcagatc gggccgcagc actggctggc catgacgtgg ctgatggcat
     9361 gcgcgggggg cggggcggca ctgggcttgg cgcttgtcct ggcgtggccg ccggcaatac
     9421 ccgttgccct ggccgcgctg gcgggagccg gttggccggg cttgtggctg cgcagccagg
     9481 cgatgcggcg caaacgccgc atcgaccggg acatgccttt cgtgctggac atgatgacgc
     9541 tctgcgtcga ggccgggctg ggcctgcatg gcgccttgca gcaatccgtg cagtgcagcg
     9601 cgcccggccc tttgcgcgac accttggacg gcgcgctggc cgacatgcgc acgggcatgt
     9661 cgcgcatggc cgcgctcaag gcgatggcgg gacgcagcgg cagtgcatcc ttgcaggcct
     9721 gggcggcttc catggcgcag gccgacgcgc tgggcatgag cctgggcccc ttgatgcgcg
     9781 gccaggccgc gcaatgccgg gccgatcgtt ttcagcatgc cgagcgcctg gccatggagg
     9841 cgccggtcaa gatgctgctg ccgctgatcg gctgtatctt tccttgcacg ttcatcgtgc
     9901 tgggattccc gattgcctgg caattgttgc cggcgttgca gtagcaggca tggccatgaa
     9961 gcatccgccc gacggcaagc cgctgcgcct ggtgcgcctg catacgcact gggcgcggct
    10021 gtgggggttg ttgtgccggc gccggccgcc ggggcggcgc tgcggcgtct ggctcgcgcc
    10081 ttgccgggcc gtccacaccc tgggcatggc ctacgccatt gacgtggctt ttattggcaa
    10141 gcagggcaag gtgctgcgag ccgttcggct cgcgccctgg cgcatggcgc tatgcgtgcg
    10201 cgctgtcggg gtggtcgaga cccgtgcggg tgtcgtcgtc gatgccgaaa accgaggtgt
    10261 aagccgggta gaagctgcaa tacagcacgc cgcccgcaat gatgttcacc ggcacctgca
    10321 ggctgtcgac cagggtgccg ggcatgccgg ccgtcaccag caggccggcg atcaggtcga
    10381 tgaacatgaa gatgagggcc caggccacgc cgtagaccag gaaggcccat ttgcagcgcc
    10441 agcaggccac gatggaaaag aatagcgact gcatcaggcg cacgccatgc caggccacca
    10501 ggacgggcgc atgccagaac agcgcggcaa tgggcacgta cagcgcgacg aacaccagca
    10561 tgggcttgcg catggccatc atcagcgggg ccaggtcctg cgcggtggag gccacgctca
    10621 tgccgtccat cacgtcgtcg gcaaagggca gtacggcgac gagttcggct gcgatgcaga
    10681 aggcggtgta gagcagcccc atgagcatga gctttctgaa tacgccgggg cgcagcaggg
    10741 gcttgggcca catcgacggc agcatgatgc gcccggcctc gacgtgcttg caggccgcga
    10801 gcgtcatgac ggtgatgacg ggcatcagca gcggcagcag cagggcgccg atgggcaggg
    10861 tgagcgcggc gaataccacg aacaggctga tcaccatggc ccaggtgaac atggccaggg
    10921 gctgcttgcg gaacaattgc aggcctgcgc gtatccattg ccagccggag gttgcaggga
    10981 gcgaggctgc ttgcatgagg tatgaggacg gtgaataaag gggccgtgag gcggtgggtc
    11041 aattatcggg cgtcgcggga tcagggcaag gccggcggtg ccagtcggtg gcgcgccagt
    11101 aacacccgct cgaaatgggt cgggtcgttg tgcttgagcg tctgggccgg acgggggaga
    11161 aaaaaatcgt aaagtcgcga cagccagaag cgcagggccg cggcgcgcag catcagcggc
    11221 caggcctggc gctcggcgtc ggtgaagggg cgcacggcgg cataggcggc cagccatgcc
    11281 tgggccagct cgggtatgaa ctcgccggtt tcgtgcacga tgcaccaatc gttcaggcat
    11341 acggccacgt cgaacagcca ggtgtcgcag ccggcaaagt agaaatcgat gaaaccgccc
    11401 atccgcgggc tgtcgtgggt gccggcaaac agcgcgttgt cgcggaacag gtcgcagtgc
    11461 gccgggcccg cgggcagggc aggccatgcg gcgcaggctt cgacctgcgc ggcgatttcc
    11521 gcgtcgagca gctcggtttg ggccggattc aggaaaggac ggaccttggg ggcggtctgg
    11581 cgccaccagt ccagcccgcg cagattgggc tgccggatgg cgaagtcgcg gccggccagg
    11641 tgggcgcgcg ccagggtggc gccggccagc ccgcagtggg ccttgccggg ggcgggttcg
    11701 tagctgccgg gcaggcgggt gacgatggcg cagggcttgc cgttgagttc ggtcaggcgc
    11761 gtgccgtcat ccagggtctg gggctcgggc acggggatgc cgcgctgcgc cagatgatgc
    11821 atgagctcga tgtagaacgg cagctgttcg cgcgtgagca cttcgaacag ggtcagcacg
    11881 tactcgccgc ggctggtgcc gaggaagtag ttggtgtttt cgatgcctga cgtgatgccg
    11941 cgcagcgaca ccagggttcc caggttgtag cgcgccagca gggcgcgcgc gtcgtcgtct
    12001 gatacagggg tgaaaacggc cattgggtgc aggcagggtt acgtcaggat tcagcgtttg
    12061 cgacgggttt gcgatcgaca tcgcccgctt gagtggggga ttttagacta cctgcccgtt
    12121 acgcattccg cgccaaccgt atccccgtga actgccagcg tgccccgggg ggcaggaagt
    12181 gccggtaggt agtacggatg agcgaacccg gcgtgatgca ggatccgccg cgcagcacgt
    12241 actggttgca catgaatttg ccgttgtatt cgccctgcat gccggcggga ggctgatggc
    12301 cggggtaggc gtcataggcg ctggacgtcc attcccaggc atcgcccagg agctggacgg
    12361 gcccgtggcc attgcgagcg agcatgggca cgggcgcggg gcggggctgc agcaggccgt
    12421 tttcgagcag gttgggccgg tcgccgttgc cttccttgcg caggcgggcg gcatgttccc
    12481 attcggcttc gcgcggcagg cgcacgcggg cccagcgcgc ataggcatcg gcctcgaaat
    12541 agcttacgtg cacgacgggg gcgtgcgggt ccagcgcctg catgccgtgc agggtgtaga
    12601 cctgccaggc gtctttttgc ccttcccagt agagcggcgc cttccagccg gcggactgca
    12661 cggcttccca gcccagggac agccatatct cggggcgccg gtagccgccg tcgagcatga
    12721 attccaggta ttcgccctgg gtgaccaggc ggtcggccag gtagtagggc ggtagcagca
    12781 cgccgtgcgc gggatgttcg ttgctgaagg caaagcctgc gccttcatgg cccagccgga
    12841 cctggccgcc ggcatagtgc gtccaccctg cgggcgtgcc ggccggcagg gcgccgggtg
    12901 ccggcgcctg cgcataggcg gggcgcagcg ggttgcagga cagcaggtgc ttgatgtcgg
    12961 cgagctggcg ctcctggtgc tgctgttcgt gctgcagggc cagatcgaag aaggcgcctg
    13021 cctggtcgcc gagcccgccc ggacgggaca gcagcgcgtg catggtggca tcgacatggg
    13081 cgcggtagtc gagcacttcg gccagcgaag gccgcgtcag caggccatgg ctggcggggg
    13141 gaagtttgct gtcgcccacc tcataggcat gaaagagctg gcgcaggcgc ggatcgtaag
    13201 gagggcggcc ggggtcggcg cgctgcacca cgaaggtctc gaaaaaccag gtggtatggg
    13261 ccaggtgcca tttgaccggg ctgcagcagg gcatggattg cgcctggcag tcttcggggc
    13321 tcaggggctg ggccagcagg aggctgagct ggcgaacggc gtcgtagcgt tgcgcggcac
    13381 tggccggggc gggcgtagcg gccggcatgg cgccgggcag gaatggatcg cgggcaaccg
    13441 cgtgggccgt cgaaccggga tgggtcagca ggcagggaag ctccatcggg cgctcctggt
    13501 ggggcacggc atcgggagta cgacctgggg tgctgcgtgg ccgtccattg tgcccggctg
    13561 tggccgggac gtgtcaagcc gggaatctgg attgcaatgg acttttacga aattcgcgat
    13621 cttgcggggg ggagggatgg ggacggaaat actgctttac ggctggttgc caaagcacag
    13681 gcaaggcagc gaggcaagta gtaagaataa tgtcatcgtg aaaacggaag gtcgggagac
    13741 tcaggcatgt cgacgatggt gatagcgcgg ttcgaaagcg tgccaatggc gcgtagtgcc
    13801 gcgcatgcct tggtcgcgga cggcttttcc gaagacgtcg tcagtgtgtt ttacggccgg
    13861 cgcccggcca ggagccgtgg cttgtgggcc cggctggtcg aggccgtgcg gcggcgcagg
    13921 cggtcggccg gcgagatcct gctcgtggtg cagctgtctt cggccgacgt ccaggcggcc
    13981 atcggcctgt tacgggatgc gggcagcgtg tccgtggagc agtcgcagga tgattggcag
    14041 acggggccct gggaaagggc ggccgtgcac cgcatccggc cggtagcgcg ccggccggcc
    14101 ggacccgggc cggtggcgca ccgcgcgcag tggcagcagc tctgatccgg gccggggtca
    14161 ggcggcgacg ggctgttgcc gcggctgcgc gagcggcacg tcgttgatcg agcaggccgg
    14221 ctggaaggaa tcggcctggg ccatgtgcca gcctatcctg aagacatcat ggcggaattc
    14281 gccgcgtagc agttcgcccg gcgacagcgt ggggaacagg gccgacagca ggcggacctc
    14341 ggcgggcgag atgcggcggg cgatgtgctg cggccgcagg tcgccggggt gggtcagccc
    14401 ggcggcgcag agcagctcgg ccagggcgtg cagcgtattg cggtggaagt tgtgcacgcg
    14461 ctgggccttg tccgggacca ccagggcgcg ctggcgcagg ggatcctggg ccgttacgcc
    14521 ggtgggacat ttgccggtgt ggcaggcctg ggcctggatg cagcccagcg cgaacatgaa
    14581 gccgcgggcg gcattgcacc agtcggcgcc catggccagg acgcgggcca tgtcgaaggc
    14641 ggtgatgatc ttgcccgagg cgcccagttt gatgcggtcg cgcaggttgg cgcccaccag
    14701 ggtgttatgc accaggcgca gggcttcgcg cagcggcgtg cccacgtggt cgacgaattc
    14761 caccggcgcg gcgcccgtgc cgccttcggc gccgtcgacc acgatgaagt cgggcgtgat
    14821 gccggtctgc agcatcgcct tgacgatggc gaaccattcc cacgggtggc ccatgcagaa
    14881 cttgaagccc acgggcttgc cctgcgagag cttgcgcagc cgcgccacga agtgcagcag
    14941 gccgatgggc gaatcgaagg cgctatgcgc ggccggcgag ttgcagtcct gccaggccgg
    15001 cacgccgcgg gcctcggcga tctcgatggt gaccttgccg gccggcagga tgccgccgtg
    15061 gccgggtttg gcgccctgcg agagcttgat ctcgatgagc ttgacctgcg gtgtgcaggc
    15121 gttgcgcaca aaggcctctt cagagaagcg gccctgttcg tcgcggcagc cgaaatagcc
    15181 cgagccgatg ttccagacca gcgagccgcc ggctttgcgg tggtagcggc tgatgccgcc
    15241 ttcgccggtg tcgtgggcga agttgcccag gcgggcgcct tcgttcaagg ccagcacggc
    15301 attggccgac agggcgccga agctcatggc cgagatgttg aagaccgaca tggcataggg
    15361 ctgcttgcag tcggggccgc ccaccatgat gcggaagtcg gtgtcttcga tgtgggcggg
    15421 ggcgagcgaa tggttgatcc attcgtagcg gtcgctgtag acgtcttcct gggtgccgaa
    15481 agggcgtttg tcgagttctt ccttggcgcg ctggtagacg atggagcgct gcgcgcgcga
    15541 gaacggcgag gcattggtgt cgtcctcgag gaagtactgg cggatctcgg gacggatgaa
    15601 ttcgaagagg aagcgcagat tgcccagcac cggatagttg cggcggatgg cgtggcgcgt
    15661 ctgcagcatg tcgtagatgc ccagcaggga gagggccccc agcgggatca ccgcccacag
    15721 ccagtatgcg gtgtaacgca cggccagcag ggccgtgacg atcgtgccga tgaggatcag
    15781 ggcgaaggtc gtgtagcggt tgacgatcca gcgcatgcat atctccacgt gacgggaacg
    15841 gcccaccata acagaacctg attacaggaa cgtgccccca cgccgcgccc gcggcttgct
    15901 gcctatcgat ggggccgctt ttgcatgatg cgggccatgc gttcagcagc tcgagggcgt
    15961 cggggtcttc tcgacggcca ggccgaacag ggggcgcagg gtcacgccga gggcgctgcc
    16021 gacgaaggcg gcgggcagcc atagccagcc gtgcaggctg ccggacagga tgccgctgaa
    16081 gtaggcgccg atattgcagc cgtaggccag gcgcgaacca aagcccagca gcaggccgcc
    16141 cacgacggcg ccgactagcg agcgggcggg caccttccag accggagcga acttgcctgc
    16201 cgcgctggcg gcggccagcg cgccgaccat gatgccgatg tccatcacgg tggtgacgtc
    16261 gtggctgacc gggtcggcca gcgcggcttt ctgggacgcc cagaactgcc acccgccgat
    16321 gttcgtgccg aaggcctgga gcaccttgac gccccacagg gcgaaggccg aggtgatgcc
    16381 ccaggggcgg ccggccagcg ccagggtggc gaagttcagc agcaccagca ccacgccgcc
    16441 ggcgaccagg ggccaggggc cgcgcagcag cgaaggcgga cggttggtgc ccgtggtggt
    16501 ggagatcaca tggccgtggc ggcgcttctc gagcaccagg gtgatccagg cgatgagggc
    16561 gaagacgacc aggttggcga ccagcgccgt gggcacgccc cagcttttga ccagcgaggt
    16621 gggcggcagg gcgggcaggg ccgaccacca gccgtaggta taggtggcca gcacactgcc
    16681 cacgatgaag aacagcaggg tgactatcat gcgggtattg ccgccgccga ccgcgaacag
    16741 cgtgcccgag gcgcagccgc cgcccaactg catgcccagg ccgaacagga aggcgcccag
    16801 gagcaccgat acgccggccg gcgacaccag tccgaccacc ggctggccga acagcgaacc
    16861 ctgcgacaga aaagggaaga acagcagcac gccgaaagcc agcatcacca tctgcgcgcg
    16921 caggccggcg ccgcggcgat cggacacgaa cacgcgccag gcctgggtga agccgaaagc
    16981 ggcgtggtag agcgtgacgc ccagcaggcc gccgacgatc cagagcgcgc tctggcgcca
    17041 gctgacggtc tgcagcaggt aggcggcgcc ggccagccag atcagcaggg cgacgaggag
    17101 cggtttgcgg ttggcgaaaa tgggcggcag cgaggccggc aggggcaggg cggaagcgtg
    17161 ggtgctcata ggaagaggat gcgcgcggga gcgtcaggca tgcgggacgg attcgacttg
    17221 caaaccggct tgcaaataac gcacgcgcat atggcaggaa aaaaagataa cctgatttta
    17281 tgacgcgaga ggcaggctct ctcatttcca ttgattatga gcttatgaca gaaatccctc
    17341 tttttccctt gggcagcacg ctgtttcccg cgggcgtcct gcacttgcgc atcttcgagg
    17401 tgcgctacct ggacatggtc aagcgctgta ttgccgaggg ttcgtccttc ggcgtggtgg
    17461 cgctgtatgc cggtggcgag gtgcgcgccc cgcatggggg cgaagtcctg tcgcctgccg
    17521 gtaccctggc ttgcatcacc cagtgggagg cgccggcccc gacgctctat cagctcacct
    17581 gtacgggcag cacgcgtttt cgcctgttgc gcagctggca ggccaagtat ggcttgtgga
    17641 tgggcgaggt ggcgtggctg cccgacgatc cggtagagga gattccggcc gaactgcagc
    17701 ccagcgccga tgccctgggc cgcttgatcg ccggcatgca gcagcagggg gtgccgtcgg
    17761 cccagatgcc cctggcgccg ccgttccggc tggacgaatg cgcctgggtg gccaatcgct
    17821 ggtgcgaact cctgcccttg ccggctaccg agcaggtgca tctcctggaa ctatccgacc
    17881 cggtcgagcg gctgcgcgcg gtgcacgccc tgctgagcgg catgctgccg ccgcaggctt
    17941 agcgcccatt caatcggcgc aattcaggcc gcgttgcgcc ggggcaggtg ctcgatggtt
    18001 tccttggccg tgcccacggc gagggagtcg gggagattga cgccgttctt gtaggccacg
    18061 atttcggcca ggatggacaa ggcgatctcg ggcggcgtgc ggctgccgat gtacaggccg
    18121 gccgggccgt gcaggcgcgc cagctgtgct tcggtgatgt cgaaatactg gcgcaggcgt
    18181 tcgcgccgcc tggcgttgtt ggcgcggctg cccagcgcgc ccacatagaa agcctcggac
    18241 tgcagcgcgt gcatgagcgc caggtcgtcg agcttggggt cgtgcgtgag cgccacgacg
    18301 gcggtgcgtg ggtcgggctg ggcggctatc accacgtcgt cgggcatgtc gtaagtgagg
    18361 tgcacgcccg ggatgttcca gtcggcccgg tactcttcgc gcgggtcgca gacggtgacg
    18421 tcgaaatcca gccctgtggc gatctcgcac aggtagcgcg agagctgtcc ggcgccgatg
    18481 acgatgagcc ggttgctcgg acccagcgtg tacgagaagt gttcttcctg caactgcggc
    18541 gggacttcgg ccgaggcgca gcccagggtg gccgcgccgg tgcgcatgtc gatgtcgcgg
    18601 cgcagggcct ggcgctgttg caggatgcgc accgtgtcgt cgatgcggct gtgcgcggcc
    18661 aggggctcga gcacgagttg cagggtgccg ccgcagggaa tgccgaaacg ccgggcttct
    18721 tcggcctgga tgccgtagat ggccacggcc ggccggttgc cggggcagag cgatgcgatg
    18781 ccggtctggc gcacccggtc gatgaggtcg tcttcgatgc agccgccgga gaccgagccg
    18841 gacacgaccg cgtcgtcacg gatggccatc atggcgccgg gcggccgggg cgaggagccc
    18901 caggtgcgca gcactgtcag cagcacggcc ttgcggccct gcctgagcca gccctggcag
    18961 gccagcaaaa cgttgagatc ggcagcttgc atggcgtggt gttgcgttca gccgcagcgc
    19021 agcgcctcga tcgcgccctt ggcatcgagg atgatgttga tgcgcgcggg gttgtattcc
    19081 atggtcatga cctggccggg cttgagcagc cgaacggcgt tcgagcccga gcgctggcgc
    19141 accgattcgc ccacgctgtc gccgtaggcc tggcccagca tgttttgcac gggctgggcg
    19201 tcgcagtctt tcgatggcgc gtcggggccg gacgcggacg cagagcccga ataggaggcg
    19261 ctcgagtagg agcccgtgcc gccggtggcg ccggcggcag agcttccgtt cgagccgggg
    19321 gcggcgcaac cggccagcag ggcagtcagc gcaagcggca tcaaggcgtt cagcaatctg
    19381 gaaaccatgg tcttccctgg gtgtgacggc aagagtctag gcattacggt agcagatgcc
    19441 gcggggctca ggccaccggc gtgcgcaggg tgacgaactc ttcggcggcc gtgggatgga
    19501 tgccgatggt ttcgtcgaag atgcgcttgg tggcgccggc cttgagcgcc acggccaggc
    19561 cctggatgat ctcgccggca tccggcccca ccatgtggca gcccagcacg cggtccgtct
    19621 cggcgtcgac caccagcttc atcagggtct tttcctggga atcggtgagc gtgagcttca
    19681 tggggcggaa gcggctctcg aagatcttga ccgtatggcc ggtggcgcgg gcttgttcgg
    19741 ccgtcaggcc gaccgtgccg atattgggca ggctgaagac cgcggtgggg atcaggctgt
    19801 agtcgacccg ctggtactcc tcgggccgga acagctggcg agcgatggcc atgccctcgg
    19861 ccagggccac cggggtgagc ggcacgcggt tgatgacgtc gcccagcgcc aggatggaag
    19921 gatcggcggt gcggtagtcg ccgtcgacct cgatgaagcc gtccttgtcg aactgcacgc
    19981 gggtgttttc caggcccagg ttgtcgacca tggcgcggcg gcccgtggca tagaagacgc
    20041 aatcggcgtc cagcgcagcg ccgtcctgca gcgttacgcg cagcgtgccg tcgggcagct
    20101 tgtcgatgcg cgccggatcg gcgttgaatc gcaggttcac gcccttcttg gcgagctcct
    20161 cgcgcaggtg ttcgcgcacg ccgttgtcga agccgcgcag gaacagcgcg ccgcggtaga
    20221 gctgcgtgac ctgggcgccc atgccgttga agatggaagc gaattccacg gcgatgtagc
    20281 cgccgccgac caccaccacg cgccgcggca gggtctccag gaagaaggcc tcgttggacg
    20341 tggtgatgtg ctcgcggccc gggatctcgg gcacgaaggg ccaactgccc gtggccacca
    20401 ggatgtgctt ggccgtgtgg cgctggtcgt tgatctcgat ggtgtgcggg tcgacgatgc
    20461 gggcgtggcc ttcgtgcagg gtgacgccgg cattgaccag caggctgcgg tagatgtcgt
    20521 tcaggcgctg gatttcgcgg ttcttgttgg cgatgagcct gggccagtcg aagcgcggcg
    20581 cctcggccgt ccagccgaaa ccggccgcct gttcgaaatc ctcggcgtag tgggcgccgt
    20641 agaccagcag cttcttgggc acgcagccca cattgacgca ggtgccaccc aggtagcggc
    20701 tttcggcgat ggccgcgcgg gcgccgaagc tggcggcaaa gcgcgaggcg cgaacgccgc
    20761 ccgagccggc gccgatgaca tagaggtcgt agtcgtaatt catggtgcga gggtgggggc
    20821 gccgtggcgg cgccctggca gatattggag attagtcctt attaaaccgc ttttgccgcc
    20881 gccgctggcc aaagacccct gggcgcggat gccgggcggc tgttttcgcg ccggtttccg
    20941 tgccggtttt gctctatcgt acggggctgc ttcacgggtt cgtttcaggg attcactgag
    21001 gagatttcaa gcatgtccgc cgtgcgcacc aatgaatacg ggctgcccat cggccccgaa
    21061 ctgccgggct ggagcgcgcg cgcgcgcccg ccgtgcacgc ccatccaggg ccgcttctgc
    21121 cggatcgagc cgctggcccc cgcgcgccat gccgacgacc tggtcgccgc cttccggcag
    21181 gcgcccgacg gacgcgcctg gacgtacctg gccgtcggcc ctttcacgga tccggccgag
    21241 catcgcgcct ggctggccaa ggccgcggcc ggcgaggacc tcctgca
//
LOCUS       DASWPO010000209         9358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1207890, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000209 DASWPO010000000
VERSION     DASWPO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207890"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VGC69_10965"
     CDS             complement(<1..358)
                     /locus_tag="VGC69_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY0295841.1"
                     /translation="MIRKHRAAFIAVAALFALLALLIAGAVAAVTWVDLNRFKPQIES
                     RVSAALGRPFAIRGDLSVAWAREPATPGWRGWVPWPRVSANDITLGNADWAQTPVMAS
                     LKRVQFGLSPLPLLAHR"
     gene            484..885
                     /locus_tag="VGC69_10970"
     CDS             484..885
                     /locus_tag="VGC69_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0295842.1"
                     /translation="MPDSAPSTAAPTPDGNPAGFNPCLDCGACCAHFRVSFYCGEVAG
                     ANGGSVPEALVTQLSPLRVCMRGTEQGGGRCTALRGELGKPGIHCAIYPLRPTPCREF
                     NIWETDGSVNPDCQRLRRGLGLPELAPRPGN"
     gene            1015..2328
                     /locus_tag="VGC69_10975"
     CDS             1015..2328
                     /locus_tag="VGC69_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295843.1"
                     /translation="MTQSHIEVIDAKRRIKAIFIGSIGNLVEWYDFYAYTAFALYFAQ
                     AFFPSSDPVVQQLNAATLFAAGFIVRPVGGWLFGLIADRYGRRLSLMTSVVMMCFGSL
                     IVAFTPTYASIGFAAPALLALARVIEGLSLGGEYGASAAYLTEVADAEHRGFYSSFQY
                     VTLIGGQLTAIIVLLVLQNIFLTHDQLVAWGWRIPFVIGALLAIIAAVMRRNLPETEN
                     FTKSLESGAARKSSAGSLRGLARYPREVALVVGLTAGGTAAFYTFTTYMQTFVKQTVG
                     LSDNTTTYVIFATLIFASILQPLYGALSDRIGRKPVLLFFGIAGTLLTVPILTTLAGT
                     KSPFIAFLLICAAWIFTAGYTSINAVVKAELFPTSIRAIGVAFPYAITVSVFGGTAPV
                     IALYFKTHGHEQWFYYYLAAMICVSLLVYLFMRDTKHASVMDRHE"
     gene            2469..3200
                     /locus_tag="VGC69_10980"
     CDS             2469..3200
                     /locus_tag="VGC69_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295844.1"
                     /translation="MPNHFLDDFTPTGVLRASINLGNPILAKRDETGLPAGVSVDLAH
                     EFARELGAQLELVVFDSAGKSVDAVTNKQADIGFFAVDPVRGAGIAFTEPYVLIEGAY
                     LVTQDSPLQSNDEVDHPGIRVAVGKGSAYDLYLTRELKHAEIVRAPTSPAVVDTYLEQ
                     GLEVAAGVKQQLEKDARRVGGLRLLPGRFMEIRQAMGCPKDMPDSAVRFLADFVERMK
                     AGGFVAQALQRHGIQGASVAPAAHP"
     gene            complement(3271..4002)
                     /locus_tag="VGC69_10985"
     CDS             complement(3271..4002)
                     /locus_tag="VGC69_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0295845.1"
                     /translation="MISLKNVNKWYNPQFQVLKDCSTSIDKGDVVVVCGPSGSGKSTL
                     IKTVNGLESIQGGEIIVDGTSVAAKGTNLSKLRSRVGMVFQHFELFPHLSITQNLCLG
                     QEKVLGRSHGEAVDKALKLLDRVGLKAHADKFPGQLSGGQQQRVAIARALVMDPICML
                     FDEPTSALDPEMVNEVLDVMVELAGEGMTMMVVTHEMGFARKVADRVIFMDAGEIVED
                     ESKESFFQAPKSDRARQFLSKILSH"
     gene            complement(4039..4731)
                     /locus_tag="VGC69_10990"
     CDS             complement(4039..4731)
                     /locus_tag="VGC69_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0295846.1"
                     /translation="MFANFDFNVIYRSLPYLFREGMSFTLMLTVIATLGGVILGTLIA
                     MMRLSSTPGLTQVATIYVNLIRSLPLVLVIFLIYFLLPYVGQWVTGASRPIQVGALSS
                     SIITFTLFEAAYFAEIMRAGIQSIPRGQIAAGYGIGLGYLQMMRYVVLPQAFRTMLPV
                     IFMQTIVLFQDTSLVYVLSITDFLGAASKVAQRDSRVTEMYMFAAVVYFIISFTASRF
                     VRRLQNRLAIVR"
     gene            complement(4734..5465)
                     /locus_tag="VGC69_10995"
     CDS             complement(4734..5465)
                     /locus_tag="VGC69_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0295847.1"
                     /translation="MNYHWDWGVFWHPTLDGSGNYLDSLLIGLQWTVVTSLCAWVIAL
                     VIGSVLGVFRTTRSSLLAGVANVYIELFRNVPLLMQLFLWYFVMPEMLPQAWGDWVKQ
                     LPNGSVYTAILGLGLYMAPRVAIQLSAGVGALPRGQRMAAQAMGLTTVQTYRYVLLPM
                     AFRIIFPPLTSEFLNTIKNSSVAITVGLLELTARARAMQEETFQVFEAFIAAAVLYLA
                     LNFIVTFAMRWIERRLAVPGYISGR"
     gene            complement(5582..6490)
                     /locus_tag="VGC69_11000"
     CDS             complement(5582..6490)
                     /locus_tag="VGC69_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0295848.1"
                     /translation="MQLKHILAGLCLAAMGTTAAVAQDLGPTLSKIKDAKAITIGYRE
                     SSIPFSYLDDKQKPIGFAMDICYKIVDAVKAKLGMPTLEVNLTPVTSSTRIPLIANGT
                     IDLECGSTTNNADRQKQVAFTQTYFLTANRVVSKKSSNIKSVEDLKGKTVVSTSGTTN
                     IQQLAEANKAKSMGMNILAAKDHAEAFLMVETGRAVGFAMDDVLLASLAASSKTPTDF
                     VITPDAFNKPEPYGIMLPKGDPQFKALVDQATTALYISPDMAKLWAKWFTSPIPPTGI
                     NLNMPMDATMTKAFAHPTDSPNTADY"
     gene            6886..7719
                     /locus_tag="VGC69_11005"
     CDS             6886..7719
                     /locus_tag="VGC69_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0295849.1"
                     /translation="MFGKLILKLVFAGALLASGAAWAADPSVDQVYQAARDGNYSQAQ
                     SMMDQVLRDHPNSAKAHFIEAELLARQGKLTAARSEFDTAQKLDSSLSFAKPGAVQEL
                     KSMLSSGGGAMSSRAGGVHVPWGPLLAILAFIVVVLMIVRSRSQRMADPGMAGGMRPM
                     PGGPMMPSGYGPGGATMAPPMAGGGMGSGILGGLATGAAVGAGMVAGEALAHRVLGDG
                     SHNVVPNNDYAQGNPGNYDMGGNDFGVNDANSWNDNSGGGGASDWGDSGGGGGSDDDW
                     S"
     gene            complement(7780..9081)
                     /locus_tag="VGC69_11010"
     CDS             complement(7780..9081)
                     /locus_tag="VGC69_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0295850.1"
                     /translation="MPTPSADSSQEILSLKNVYRGFDKSQGELLVLDGVNLSLREGEI
                     VGLLGRSGSGKSTLLRIIAGLIKPSGGEVSYLGKPLSGPAEGVAMVFQTFALFPWLTV
                     LQNVEAGLEALGVAAKERRARALAAIDLIGLDGFENAYPREMSGGMRQRVGFARALVV
                     DPTLLLMDEPFSALDVLTAETLRTDLLDLWTQGRMPIKAVLIVTHNIEEAVFMCDRIL
                     VLSSNPGKVISEIKVPFKHPRNRLDPAFRKLVDDIYAQMTTRAGQSARGGLELGSWLP
                     SVSTNLMAGLIETLATAPYNGRADLPEIARSLQLEVDELFPIAEMLQHLGFADVHQGD
                     MFLTPAAHKFAELGTQERKMMFAEHLLKNVPLAARIRAVLNERPGHRAPRVRFEQELE
                     DFLTDGAAEETLDAVIDWGRYGEIFSYNDQSEIFSLEDVES"
     gene            complement(9106..>9358)
                     /locus_tag="VGC69_11015"
     CDS             complement(9106..>9358)
                     /locus_tag="VGC69_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfonate ABC transporter permease"
                     /protein_id="HEY0295851.1"
                     /translation="YVTGAITASGGAWNASIVSEYVQWGDTKLAAHGLGAYIARTTAA
                     GDFPKIILGIAVMSLFVTLFNRLLWRPLHAYAESRLRLD"
BASE COUNT         1646 a         3037 c         3033 g         1642 t
ORIGIN      
        1 cccgatgcgc cagcagcggc aatggcgaca agccgaactg cacgcgcttc aggctggcca
       61 tgaccggcgt ctgcgcccag tcggcgttgc cgagcgtgat gtcgtttgcg ctgacacgcg
      121 gccacggcac ccagccgcgc cagccggggg tggcaggttc gcgcgcccag gctaccgaca
      181 gatcgccacg gatggcgaac gggcggccca gcgcggccga gacgcgcgac tcgatctgcg
      241 gcttgaaccg gttcaggtcc acccaggtca cggccgcaac cgcccccgcg atcaggaggg
      301 caagcagcgc gaacaaggca gccacggcga tgaatgctgc gcggtgtttg cggatcatgt
      361 gtgctttgct ccgggccggc acacatccgg ccccttatat ttagggtagc gtagccagcc
      421 ggccgatggc gcatactgca ggcaaccgat acatccggca aagctcaggt acactcgcgc
      481 gccatgccag acagtgcgcc ttccaccgcg gccccgacgc ccgacggcaa cccggccggc
      541 ttcaatccct gcctggattg cggggcttgc tgcgcgcatt tccgcgtgtc cttctattgc
      601 ggcgaagtcg ctggcgccaa cggcggcagc gtgccggaag cgctcgtcac gcagctgtcg
      661 ccgctgcgcg tctgcatgcg gggcaccgaa cagggcggcg gccgttgcac ggccctgcgc
      721 ggcgagctgg gcaagcccgg catccactgc gccatctatc cgctgcgccc cacgccctgc
      781 cgcgaattca acatctggga aaccgatggc agcgtgaacc ccgactgcca gcggctgcgc
      841 cgcggcctgg gcctgcctga gctcgctccg cgccccggca attgatgcaa tccggaccgg
      901 caggaagctg tctttaagct gaatttaagt cgatagttct ttcaatgtgc tatcgaacgg
      961 gcgcaaaatg cggcccgggg gattccatcc ataaattcca aaagcgaggc agcaatgact
     1021 cagtcccata tcgaggtgat cgacgcaaaa cgtcgcatca aggcgatctt cattggctct
     1081 atcggcaacc tggtggagtg gtacgacttc tatgcctaca cggcctttgc gctgtatttc
     1141 gcgcaggcgt tctttccgtc gagcgacccg gtcgtgcagc agctcaacgc ggccaccctg
     1201 ttcgccgccg gtttcatcgt gcgcccggtg ggcggctggc tcttcggcct catagccgac
     1261 cgctacggcc ggcgcctgtc gctcatgacc tccgtggtaa tgatgtgctt tggctcgctc
     1321 atcgtggcct tcacgcccac ctacgcctcg atcggctttg ccgcgcccgc gctgctggcc
     1381 ctggcccgcg tgatcgaggg cctgagcctg ggcggcgagt atggcgccag cgccgcctat
     1441 ctgaccgaag tggccgatgc cgagcatcgc ggcttttatt ccagcttcca gtacgtcacg
     1501 ctgatcggcg gccagctcac cgccatcatc gtattgctgg tgctgcagaa catcttcctc
     1561 acgcacgatc agctcgtggc ctggggctgg cgcatcccct tcgtcatcgg cgccctgctg
     1621 gcgattattg ccgccgtcat gcgccgcaac ctgcccgaga ccgagaactt caccaagtcc
     1681 ttggaatcgg gcgcggccag gaagtccagc gcgggctctc tgcgcggcct ggcgcgctac
     1741 ccgcgcgagg tggccctggt ggtgggcctg acggccggcg gcacggcggc cttctacacg
     1801 ttcaccacgt atatgcagac cttcgtcaag cagaccgtgg ggctctccga caacaccacc
     1861 acctacgtga tcttcgccac gctgatcttc gcctcgatcc tgcagccgct gtatggcgcg
     1921 ctgtcggacc gcatcggccg caagccggtg ctgctgttct tcggcatcgc gggcacgctg
     1981 ctgacggtgc ccatcctgac gacgctggcg ggcaccaagt cgcccttcat cgccttcctg
     2041 ctgatctgcg cggcctggat cttcaccgcc ggctatacct cgatcaacgc cgtggtgaag
     2101 gccgagctct tccccacctc gatccgcgcc atcggcgtgg cctttcccta cgccatcacc
     2161 gtctcggtgt tcggcggcac ggccccggtc atcgcgctgt atttcaagac gcacggccat
     2221 gagcagtggt tctactacta cctggccgcc atgatctgcg tgtcgctgct ggtctacctg
     2281 ttcatgcgcg acaccaagca cgcctcggtg atggacaggc acgaataggc gtaaacagcg
     2341 tacagggaca tgcggccgcc cccgccgcat gggatgagat acaaaaaagc ggccaggccg
     2401 gataagcctg gccgcttttt tatcgctatc atctttttac cgccgcgagc catcagccag
     2461 ggacagccat gccaaaccac tttctcgacg acttcactcc cacaggcgtc ctgcgcgcct
     2521 ccatcaacct gggcaacccc attctcgcca agcgcgacga gacgggactg cccgccggcg
     2581 tctccgtgga tcttgcccac gagttcgccc gcgagctggg cgcgcagctg gaactcgtgg
     2641 tgttcgactc cgccggcaag tccgtcgacg cggtcacgaa caaacaggcc gacatcggtt
     2701 tcttcgcggt cgatcccgtg cgcggcgcgg gcatcgcctt taccgagccc tatgtgttga
     2761 tcgagggcgc ctacctggtc acccaggact ccccgctgca gagcaacgac gaagtcgacc
     2821 accccggcat ccgcgtcgcg gtcggcaagg gcagcgccta cgacctgtac ctcacgcgcg
     2881 agctcaagca cgccgagatc gtgcgcgcgc cgacctcccc cgccgtggtc gacacctatc
     2941 tcgaacaagg gttggaggtt gccgcgggcg tgaagcagca gcttgaaaaa gatgcccggc
     3001 gcgtgggcgg cctgcggctg ttgccagggc gcttcatgga aatccggcag gccatgggct
     3061 gccccaagga catgcccgat tccgccgtgc ggttcctggc ggattttgtc gagcgcatga
     3121 aggccggcgg gttcgtcgcg caggccttgc agcggcatgg catccagggg gcgtcggtgg
     3181 cgcccgcagc gcacccctga atgccgggcg ccggccggcg ccgccctgtc agggcgacac
     3241 tttcagtgcg acaccttcag tgcgacactt tcagtgcgac aggatcttcg acaggaactg
     3301 gcgtgcgcgg tcgctcttgg gagcctggaa gaacgattcc ttgctctcgt cctcgacgat
     3361 ctcgccggcg tccatgaaga tgacgcggtc ggccaccttg cgggcgaagc ccatttcgtg
     3421 ggtcacgacc atcatggtca tgccttcgcc ggccagttcc accatcacgt ccagcacttc
     3481 attgaccatt tccgggtcga gcgccgaagt gggttcgtcg aacagcatgc agatggggtc
     3541 catcaccagc gcgcgggcga tggccacgcg ctgctgctgg ccgcccgaca actggccggg
     3601 gaacttgtcg gcgtgcgcct tcaggcccac gcggtcgagc aacttgagcg ccttgtccac
     3661 ggcttcgccg tgcgaacggc ccagcacctt ttcctggccc aggcagaggt tctgcgtgat
     3721 gctcaggtgc gggaacagtt cgaagtgctg gaacaccatg cccacgcgcg aacgcagctt
     3781 ggacaggttg gtgcccttgg cggccaccga ggtgccgtcg acgatgatct cgccgccctg
     3841 gatggactcc aggccgttga cggtcttgat gagcgtggac ttgcccgagc ccgacgggcc
     3901 gcacaccacc accacgtcgc ccttgtcgat ggaggtgctg cagtccttca gaacctggaa
     3961 ctgcgggttg taccacttgt tgacgttttt cagagaaatc atggacacga aatggccctc
     4021 cggcgtgtcg gcatacgatc aacgaacgat cgccaggcgg ttctgcaacc gccggacgaa
     4081 cctcgatgcc gtgaacgaaa tgatgaaata gaccaccgcc gcgaacatat acatctcggt
     4141 gacgcggctg tcgcgctggg ccaccttgga cgcggcgccc aggaagtcgg tgatcgacag
     4201 cacatacacc agcgaggtgt cctggaacag cacgatggtc tgcatgaaga tcaccggcag
     4261 catggtgcga aaggcctgcg gcagcaccac gtagcgcatc atctgcagat agcccaggcc
     4321 aatgccgtag cccgccgcga tctgccccct gggaatggac tggatgcccg cccgcatgat
     4381 ctcggcgaaa taggctgcct cgaacagggt gaaagtgatg atggacgagc tgagcgcccc
     4441 cacctggatg ggccgcgatg cgccggtcac ccactggccc acgtagggca gcaggaagta
     4501 gatcaggaag atcaccagca ccagcggcag cgagcggatc aggttgacgt agatcgtggc
     4561 gacctgggtc aggcccggcg tcgacgacag gcgcatcatg gcgatcaggg tgcccaggat
     4621 cacgccgccc agcgtggcga tgaccgtgag catcagcgtg aagctcatac cctcccggaa
     4681 caggtagggc aacgaccggt aaatgacgtt gaaatcgaaa ttggcgaaca tggtcagcgc
     4741 cccgagatgt agccgggcac tgccaggcgg cgttcgatcc agcgcatcgc gaacgtgacg
     4801 atgaagttga gcgccaggta cagcaccgcc gccgcgatga aggcctcgaa cacctggaag
     4861 gtttcttcct gcatggcgcg ggcccgcgcc gtgagctcga gcaggccgac ggtgatggcc
     4921 accgagctgt tcttgatggt gttgaggaac tcggaggtca gcggcgggaa aatgatgcga
     4981 aacgccatgg gcagcagcac gtagcgatag gtttgcaccg tggtcaggcc catggcctgg
     5041 gccgccatgc gctgcccgcg cggcagggcg ccgacaccgg ccgacaactg gatggccacg
     5101 cgcggcgcca tgtacagccc cagccccagt atcgccgtat aaaccgagcc attgggcagt
     5161 tgcttgaccc aatcgcccca ggcctgcggc agcatctcgg gcatgacgaa ataccagaga
     5221 aacagctgca tcagcaacgg cacgttgcga aagagttcga tatagacgtt ggccacgccg
     5281 gccaacaggg aagaacgggt ggtccggaac acgcccagca ccgagccgat gaccagtgcg
     5341 atcacccacg cgcacagcga ggtgacgacg gtccattgca ggccgatgag cagggagtcc
     5401 agatagttgc cggagccatc cagcgtgggg tgccagaaaa cgccccagtc ccagtggtaa
     5461 ttcatgcgat caccctcacc aaggcagcac gcgtgcgcgg tgcgcacgcg ccttcttcgt
     5521 tacgacaggc gccacccggg cgcgacggcc cgggggtgga tcgcctcatg tcggctatcg
     5581 atcaatagtc agccgtattg gggctgtcgg tgggatgcgc gaaggccttc gtcatggtgg
     5641 cgtccatcgg catgttcagg ttgatgccgg tgggcggaat gggcgaggtg aaccacttgg
     5701 cccagagctt ggccatgtcg gggctgatgt agagcgccgt ggtggcctgg tcgaccagcg
     5761 ccttgaactg cgggtcgccc ttgggcagca tgatgccgta gggttcgggc ttgttgaagg
     5821 cgtcgggcgt gatgacgaag tccgtcgggg tcttcgagct ggcggccagg ctggccagca
     5881 gcacgtcatc catcgcgaag ccgacggcac ggccggtttc gaccatcagg aaggcttcgg
     5941 cgtgatcctt ggccgccagg atgttcatcc ccatgctctt ggccttgttg gcctcggcca
     6001 gctgctggat gttggtcgtg cccgaggtcg agaccacggt cttgcccttc aggtcttcga
     6061 cgctcttgat attgctcgac ttcttgctga cgacgcggtt ggccgtcagg aagtaggtct
     6121 gggtgaaggc gacctgcttc tggcggtcgg cgttgttggt ggtggaaccg cactccaggt
     6181 cgatggtgcc gttggcgatc agcgggatgc gggtggacga ggtgaccggg gtcaggttca
     6241 cttcgagggt gggcatgccc agcttggcct tgaccgcgtc gacgatcttg tagcagatgt
     6301 ccatggcgaa accgatgggt ttttgcttgt cgtccagata ggagaaggga atcgacgatt
     6361 cgcggtagcc gatcgtgatc gccttggcat ccttgatctt gctcaaggtc gggcccaagt
     6421 cttgggccac ggcggcggtg gtgcccatgg cagccaggca gaggccggcc aggatgtgct
     6481 tgagttgcat gcaattaccc cttgcgtgaa acaacagtta aacgagaaac gccccgcggc
     6541 gtacgctgac agagatgcac tgctgtcgca ttgctttcat ttggcctgcg acaaagcccc
     6601 cggaaagggg cccgcatgcg caagaacggc gtagtttagc agccgattat tgctctgtcg
     6661 gggtcatatc tacagtaatg acagccaatc agggcaaacc acaaggaccg tccgtttcta
     6721 tttacatttc aaaaactggt ccgatacgca ctttatgtaa catcccaata gatcgatcaa
     6781 caccgcgtgc cgcattagcg cgctacgctt gccctatccg gagcggggtt gaactttttc
     6841 gaagcaaccc catctcatat gtcccgctta tgggagctat cgaccatgtt tggaaaactc
     6901 atcctgaaac tcgtttttgc gggggcgctt ctggcctccg gcgccgcctg ggccgccgat
     6961 ccctcggtgg atcaggtcta ccaggccgcc agggacggca actacagcca ggcgcaaagc
     7021 atgatggacc aggtgctgcg cgaccacccc aacagcgcca aggcgcactt catcgaggcc
     7081 gagctgctgg ccaggcaggg caagctcacc gccgcgcgca gcgagttcga cacggcgcag
     7141 aagctcgatt ccagcctgtc cttcgccaag ccgggcgccg tgcaggaact gaagtcgatg
     7201 ctgtcgtccg gcggcggcgc catgtcgtcg cgggcaggcg gcgtccatgt gccgtggggg
     7261 ccgctgctgg cgatcctggc ctttatcgtc gtggtcctga tgatcgtgcg ttcccgttcg
     7321 cagcgcatgg ctgatcccgg catggccggc ggcatgcgcc ccatgcccgg gggtcccatg
     7381 atgcccagcg gctacggtcc gggcggcgcc accatggcac cgcccatggc cggcggcggc
     7441 atgggctctg gcatcctggg cggactggcc acgggtgccg ccgtgggcgc cggcatggtg
     7501 gcgggcgagg cgctcgcgca ccgggtgctg ggcgacggct cgcacaatgt cgtgcccaac
     7561 aacgactatg cgcaaggcaa tccgggcaac tacgacatgg gcggcaacga tttcggcgtg
     7621 aacgacgcca actcgtggaa cgacaacagc ggtggcggcg gcgccagcga ctggggcgac
     7681 agcggcggcg gcggcggttc cgacgacgac tggagctgac ctgcccacgg cgtcacgcca
     7741 ggaaaaagcc cccgagcgcg aagccggggg cttttttcat caggactcga cgtcttccag
     7801 gctgaagatc tcgctctggt cgttgtacga gaagatctcg ccgtagcggc cccagtcgat
     7861 cacggcgtcg agcgtttcct cggcggcgcc gtcggtcagg aagtcctcga gctcctgctc
     7921 gaagcgcacg cgcggcgcgc gatggcccgg gcgctcgttg agcacggccc ggatgcgcgc
     7981 ggccagcggc acattcttga gcaggtgctc ggcaaacatc atcttgcgtt cctgggtgcc
     8041 cagctccgcg aacttgtgcg cggccggcgt caggaacatg tcgccctggt gcacgtcggc
     8101 gaagcccagg tgctgcagca tctcggcgat ggggaacagc tcgtcgacct ccagctgcag
     8161 cgaacgggcg atctcgggca ggtcggcgcg gccgttgtag ggcgcggtgg ccagggtttc
     8221 gatcaggccg gccatcaggt tggtcgacac gctgggcagc cagctgccca gctccagccc
     8281 gccgcgcgcg gactgcccgg cgcgcgtggt catctgcgcg tagatgtcgt cgaccagctt
     8341 gcggaaggcc gggtccaggc ggttgcgcgg atgcttgaag ggcaccttga tctcggagat
     8401 caccttgccc gggttggacg acagcaccag gatgcggtcg cacatgaaga ccgcctcttc
     8461 gatgttgtgc gtgacgatga gcaccgcctt gatcggcatg cggccctgcg tccacaggtc
     8521 gagcaggtcg gtgcgcagcg tttcggccgt cagcacgtcc agcgcggaga aaggctcgtc
     8581 catcaggagc agcgtggggt cgaccaccag cgcccgcgca aagcccacgc gctggcgcat
     8641 gccgcccgac atctcgcggg gataggcgtt ctcgaaaccg tccaggccga tcaggtcgat
     8701 ggccgccagg gcgcgggcac gccgctcctt ggcagccacg cccagcgcct cgaggccggc
     8761 ctcgacgttc tgcagcaccg tcagccaggg aaacagcgcg aaggtctgga acaccatggc
     8821 cacgccttcg gccgggccgg agagcggctt gcccaggtag ctcacctcgc cgccggacgg
     8881 cttgatcagg ccggcgatga tgcgcaggag cgtcgacttg cccgaacccg aacggcccag
     8941 caggccgacg atctcgccct cgcgcagcga caggttgacg ccgtcgagca ccagcagctc
     9001 gccctggctc ttgtcgaagc cccggtagac gtttttgagg ctgaggattt cctggctgga
     9061 gtccgcggag ggagtcggca taatcggcat ggttttactc tacggtcagt cgagccgcag
     9121 ccgggattcg gcataggcat gcaacggccg ccacaacagg cggttgaaca gcgtcacgaa
     9181 cagggacatc acggcgatgc ccaggatgat cttgggaaaa tcgcccgccg ccgtggtccg
     9241 cgcgatatag gcgcccagcc cgtgcgcggc cagcttggtg tcgccccatt gcacgtactc
     9301 ggagacgatg ctcgcgttcc aggcgccgcc cgaggccgtg atggcgcccg tgacgtaa
//
LOCUS       DASWPO010000210        30137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_672263, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000210 DASWPO010000000
VERSION     DASWPO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 30137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30137
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672263"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VGC69_11020"
     CDS             complement(<1..1038)
                     /locus_tag="VGC69_11020"
                     /inference="COORDINATES: protein motif:HMM:NF037006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0295852.1"
                     /translation="MDKRIDVKARSGSTDTHTVAQNTQNTSIPIHDQAKVVLFATQSD
                     IASMQREGDDLLIQYADGTAVRLEGYFDCPVDAIPTLVLDDKNAADNTEQMWKAEFNP
                     AVCYAPDDATAGLVAYSWVPYADGGAAALALPILLGALGLIGGGVAIAAGGGGGGGGD
                     DNGANSTPTRPAAPSAHFNGDGSAVIGKGEPNATIKITDASGQSLGTGTADGNGDFNV
                     PLNDPQKNGEVDHVTQTTPGGTSPDTAITAPDLTAPEAPAITSATDDAAPVTGPVASG
                     GSTNDNTPTLAGTAEPHATVNIYDNGALIGTATADASGNWTFTPGTALADGAHSITAT
                     ATDAAGNTSPAS"
     gene            1334..2728
                     /locus_tag="VGC69_11025"
     CDS             1334..2728
                     /locus_tag="VGC69_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0295853.1"
                     /translation="MTRTARCVFIVAMAMAPGLGAAMPLEEAVRLAALRDPVVAALQQ
                     DVAQQETNIEIIRDGQRPRFSVNVGRATSLGSDPTLATERNIGSELVVSQLLFDWGKV
                     SAQIEGANDDRVQIVSDLKKQLESVIFSIAGLYLDAETAKAKLQATDEYRKNAVKLSE
                     MTQDRAQGGLGDVSETARAKLEISRAQERQQTFESNRDVALDQLNLLVGVSGISTQKV
                     PELAYGQHVGTSGTLAQSIEGAPDYLKSHAAMDSARTGIDVAKAATKPALRVEAAGHP
                     GFSGGGRTSGSLGLVLGVDLGVSDLYGRQAQAAEQKYEGARKRLEGVERDLKNQTQSY
                     ARQLKTLAASEAAFRLQVDEARSVVATYEDQFTAGLRNMTDLFTSIQELYSTQLSWID
                     TIDQLRRTEYKAAQALGLQGTLLQERTGMSLPDVPAFLSAEQAPQPVRQPTAPFDDPG
                     HYAKLPFNSREVTP"
     gene            2725..4893
                     /locus_tag="VGC69_11030"
     CDS             2725..4893
                     /locus_tag="VGC69_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEY0295854.1"
                     /translation="MSAADQIAMYEPGLLAGLVRLCAAHGVSTSAQRLGDGLLREPGV
                     LPFSQVERALRRVNMTCGMVALPLKRLSAHALPVLLLMRDGSCLVLEKLTPTSAEVSH
                     PESGAGRSAMPRKALEEGYAGRYVIAKHIEVVSAHLDGSFDKKPRHWILGPVRDQWHV
                     YRDVLLGSLMANLLAVGVALFSMQVYDRVVPNAAFDTLWVLSSGVIAAVLLEWVLRYM
                     RARLIDHSGRALDLQLSAQLFERVVHMRLSHQPTSIGAFANQVRDFTSVRDFFTSGTL
                     ATLCDLPFVVVLLALIGFIGGWVVAVPLVAVVLITLPGIVMQKRLAALSRQNTRESAA
                     LNGLLLEAVDNLENVKATRSEERLQRAHASLTAIMATSGSKTRDITSMLQLMAGSTQQ
                     LCYTAVVIVGVYLITDGRLTTGSMVACTLLSSRVLSPMSQVAGLLTRWQFVKAAMEGL
                     DRIISLPVERPADRHFVRAEQMRGAYRLEHVVYAHRPDSPAALHIPRLAIRAGEHVAL
                     LGGNGAGKSTLMRLLAGLTDPQQGNVLLDDLALSQIDPIDRRRQIGYLPQTVALFQGT
                     LRENLTLGHALWSDAELLKALDAVGLGPFVRKHVRGLDQDILSIGNMSGGQKQAVGLA
                     RVILQDPRIVILDEPTSAFDQANEARVVGFMKTWLDGRTALIATHKRELLVLTQRAIV
                     LRDGKVVHDGEMGQLVELATQRARRPPAAPEIKVVTGGAS"
     gene            4890..6059
                     /locus_tag="VGC69_11035"
     CDS             4890..6059
                     /locus_tag="VGC69_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY0295855.1"
                     /translation="MSRGHAMLLDVSDGGRLQRVRTSRTVWFITLILALLIAWAAWAH
                     VNEVTRGKGRVTPYSRLQSVQSLEGGILTELLVREGDIVQADQPLARIDSTRFQSAYN
                     ETQSQIDTLAAEIARLETEVNEKTELVFEGEGDAKARQAEMELFKARRTRLDKSIGAL
                     QEEDRILQRQTAMVQPLVKKQSVPESELLRLQQQSAQLHGRIGELRNQYVQEAYSDMV
                     DKKAKMAALQQSMMQKKDQLDRTLVTAHVAGRVNNVRINSVGGVVQPGETIMEISPIH
                     DQLVVETLVEPKDVAFIAPGMPASVKITAYDYSVYGDLRGKVTQISEDTVKEDTPHGP
                     QEYYRVMVTTDRAYLQKGEEQLPIRPGMVAEVDIETGSRSILNYLTRPLMKARLR"
     gene            complement(6075..7415)
                     /gene="argG"
                     /locus_tag="VGC69_11040"
     CDS             complement(6075..7415)
                     /gene="argG"
                     /locus_tag="VGC69_11040"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY0295856.1"
                     /translation="MATILPSLPVGQKVGIAFSGGLDTSAALIWMREKGAIPYAYTAN
                     LGQPDEPDYEDIPRRARQYGAENARLVDCRQQLVAEGIAALQAGAFHISTGGITYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTFKGNDIERFYRYGLLTNPELKIYKPWLDQ
                     QFIDELGGRAEMSAYMAKAGFDYKMSAEKAYSTDSNMLGATHEAKDLEQLNSGIRIVK
                     PIMGVAFWKDDVAVKAEAVTVRFEEGQPVALNGVEYSDPVALMAEANLIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLFIAYERLVTGIHNEDTIEQYRDNGRKLGRLLYQG
                     RWFDPQAIMLRETAQRWVARAITGEVSIELRRGNDYSLLNTVSPNLTYAPERLSMEKV
                     ENAPFTPADRIGQLTMRNLDITDTRDKLFTYTKAGLLLPSGGSALPQLQDSKKK"
     gene            complement(7468..8418)
                     /locus_tag="VGC69_11045"
     CDS             complement(7468..8418)
                     /locus_tag="VGC69_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295857.1"
                     /translation="MDIKQIEYFVRVAERRSFSRAAELLDVAQPTLSRQVGLLEQELG
                     QHLLYRNGRGVEPTEAGLRFLEYARALLALAARAKEDLQGLRDTPTGKVAIGLPPRIA
                     RPVTPPLVQAFRRACPDASITVAEGLSTQVREWLLAGRIDLALLYDPPASPQLAYESL
                     FREDLVLAAAPGGRLPLPAVVQVAQLGRYPLILPSLPNAIRALVESACRRQGVRLTVA
                     AEVDAVQTIVELASQGDAYAILPRSAAQGPAAAHPLVLCRIEAPVIRNDLVLAVPRHR
                     PAPRLASATAQLLRELDLKRLFVGAAAGAGTPGKRVKMRV"
     gene            8536..9417
                     /locus_tag="VGC69_11050"
     CDS             8536..9417
                     /locus_tag="VGC69_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0295858.1"
                     /translation="MTGDRFVPDCRPPLAAPSPPRRRLPEGACDTHCHVFGPADRFPY
                     AEGRSYTPPDAPFEKLAALHAHLGAAHAVIVQANCHGHDHAALLDAVARSQGRYRGVA
                     LLPPQTDAAMIRALHAGGVRGARFNFVPHLGAPPSPEAFDRMVSLIAPFGWHVCLHVD
                     GAMIGELLPRLAGLPVPFVVDHMGRIRACDGLDAPAFRALLALARVPGAWVKVSGIDR
                     IGDGRRPFHEGIPFIRALVEAMPDRTVWGTDWPHPNVAGDMPDDGEVVDAFFEACPDE
                     AARRRVLVENPARLYGF"
     gene            9508..10461
                     /locus_tag="VGC69_11055"
     CDS             9508..10461
                     /locus_tag="VGC69_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295859.1"
                     /translation="MFKRMVCAGALAWAALGAAQAEGPVQLIVAFPPGGPVDLVGRVL
                     AEQLSKALDRPVIVENKAGANGNIGAAYVSKAPGDGSVLFLTSVGAVSISPALYKSLP
                     YDPVSGFKPISKVVDNATVFVVNPSNPAVDAADFVKRSQAASQPVAIGSSGVGSIPHL
                     TLEMFADASKAKVMHVPYKGAAPVISDLLGNQVAGFFGDVPGLIGQIQSGRLKVLGLA
                     APERLPLLPQARTLAEQGIPGVETNNWYGLLAPAATPPALVDKLNAAVRAALQAPEVR
                     DKLAQFGCQVAPTSPQELADLIAADRRKWSDLIMHKNIHLE"
     gene            10466..10996
                     /locus_tag="VGC69_11060"
     CDS             10466..10996
                     /locus_tag="VGC69_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0295860.1"
                     /translation="MSPYRIAPVEPGARPELAGIEARILAARGRITPLYQVLLNSPPV
                     VDGWEAMLTAIRQKTALSPRVRELIILRVAVLNRAPYEFEAHVPHARAAGLREAVIEG
                     LRVYAAPDAVQGLDGGEREVLALTDAMTRDIEVPDAVFEPLRAHFDEQGLIELAATVA
                     AYNMVSRFLVALRIGH"
     gene            11017..11595
                     /locus_tag="VGC69_11065"
     CDS             11017..11595
                     /locus_tag="VGC69_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295861.1"
                     /translation="MQTQEILLVKVPARLDAAAAGRVRVALSDGLALSVEVCAALELD
                     ESYVYCRPRSGVDAAQALPAARGRAATACPAGSQALLLEGLLHLQSEPDGGAAPWHYI
                     VETDVTPEFEREMNDWYDREHMPGLAVVPGTVRADRYLSRDGSPRYYACYDLRTLETF
                     GSPPWLAVRATDWSSRVRPAFRNTKRTMFARV"
     gene            11609..12499
                     /locus_tag="VGC69_11070"
     CDS             11609..12499
                     /locus_tag="VGC69_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0295862.1"
                     /translation="MRIKPWLACLAAGAVFVLGAARPADAAATAAVAADAVEHHYVFA
                     GGGVRIEVLAQGSGRPLVLLPSRGRGAQDFDQLAQRFAQAGYRVLRPQPRGIGGSIGP
                     MRGATLHDYADDMAAVIEDVARQPVVMVGHAFGNWVARATAVDHPEWVRGVVIVAAAA
                     KQYPPGLSQLVDRSSDLSLPDAERLKAIQDAFFAPGHDARVWLGGWYPEVNESQNQAG
                     KATRQADWWSGGKAPMLDLQADLDKFKPPATRGEVRDEFGQRVTVAVIRGAGHALVPE
                     APQAVADAVIGWERGLPDRP"
     gene            complement(12506..13477)
                     /gene="argF"
                     /locus_tag="VGC69_11075"
     CDS             complement(12506..13477)
                     /gene="argF"
                     /locus_tag="VGC69_11075"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY0295863.1"
                     /translation="MTATPSPSNVNPSAAQGNPAPLRHFLQLKDFSSGELAYVLDRAR
                     VIKDKFKRYEPHMPLHDRTLAMVFEKASTRTRVSFEAGMYQMGGSVINLTSNDSQLGR
                     SEPIEDTARIISRMVDIVMIRTFEQARIESFAAYSRVPVINGLTNEFHPCQILADILT
                     FIEHRGPIAGKTVAWVGDSNNMAYTWLQAAELLGFTLHVCAPAGYELDPARIGAPPPT
                     VLRQFKDPMDACLGAHLVTTDVWTSMGYEAENEARRKAFADWCVDADMMAVADPRAIF
                     QHCLPAHRGEEVTAEVLEGPQSVVWDEAENRLHVQKALMEFLLLGQL"
     gene            13606..13908
                     /locus_tag="VGC69_11080"
     CDS             13606..13908
                     /locus_tag="VGC69_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEY0295864.1"
                     /translation="MTTRVRQFLIYGITESGSRFRPSDWAERLAGVMAQFRPPGVVGT
                     HLTYSPYVLPVVMDGVRGVVVDQRLRELEPLAYKFVVDFARDNNLKTEEREIDEKA"
     gene            complement(13915..14922)
                     /locus_tag="VGC69_11085"
     CDS             complement(13915..14922)
                     /locus_tag="VGC69_11085"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY0295865.1"
                     /translation="MGIITEAEYRYNMLIKQFPSKAEPAFHTPEEQIANWGRVWGCDN
                     DVGQIRKVLMHRPGDELKVIDPGKRIPGINAFGEVDKGWYWRGDVIPSLAAMQAEHDA
                     YAAVLREEGAEVVYIDQVAPGRMKQVYTRDSVIAVNGGAIVTRLGPPIRRGEELPVTR
                     TLAALGMPILRTIAGTGVLEGGSFVLLNAKTAVLGLSSRVNAEGARQLREVLATQGVE
                     LLTIELTGYRLHIDGMLVMVDVDTAFINPALLPFWFLEKLQELKIRTIEVCDEDENWV
                     VNCLAVRPGRVLMAEGLSDRTAEKFERAGVSIRVVPYANVALGGGGLHCSTAPLIRDS
                     V"
     gene            complement(14959..15753)
                     /locus_tag="VGC69_11090"
     CDS             complement(14959..15753)
                     /locus_tag="VGC69_11090"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295866.1"
                     /translation="MSSAFQQKPAMATRSLAWTIICFLVLPITILFAVSLTDRSYLSL
                     PAEHVSLQNYHKLFTSPQWLASIWQSFVIACASTAIAGTLGTLAAVGCWRIGTALANM
                     VRFAMLVPMMLPPIIYALGAYQVWSDLGWINTYTGIIVAHSVTGIPYVIVTVSTALAG
                     LDPRLEQAARNLGAGIGQTLRHVILPNIRPGIVSGLVFAFIHSWDELVIVLFIAGRTV
                     FTLPRKMWDGINEALDPTMAAVASVLILLTLALFTLDLALRARKKH"
     gene            complement(15833..16681)
                     /locus_tag="VGC69_11095"
     CDS             complement(15833..16681)
                     /locus_tag="VGC69_11095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295867.1"
                     /translation="MNVPRFRHPLLKYWLLTLPALLGLAAFYFYPIGRVLWMSVTVPE
                     PGLSNFAFVAASRSIHRMLGTTLRITLLSTVATLMLAYVVAYVIANADERRRRVLFMG
                     VVVTLWISVLVRAFAWFTLLRNDGLVNQLLMGLGIISSPLPLMWNEFAVLVGMVHYML
                     PLAILPLYSGMHAIDPRLVAAARGLGASRRRAFWRVYFPLTLPGVFGASMLVFIYALG
                     FYVTPALLGGGKTLMIAEYIKMQILDVVNWGVGSALASLLLIFILAVLAAVGRFIDLR
                     KLFGAA"
     gene            complement(16685..17794)
                     /locus_tag="VGC69_11100"
     CDS             complement(16685..17794)
                     /locus_tag="VGC69_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0295868.1"
                     /translation="MEEQTFAQDYAELMQEQRARGMSRRDLLRSALAMGVAPLAVGAM
                     GARNAFAADKPEVVLVNWGGVSVDVFKTAFADPYVAAGGKMVIDGSGPVNGKILTMVQ
                     SKNVTWDICDGGITTIAELGPKGALEPIDYSIVDKSKVMDGFAYELGVVNYFFSTVMA
                     WDTAKIQGTPTLADFFDIKKIPGRRMIRKDSQAMLEMALLADGVAIKDLYPMDVERAM
                     AKFESIKDSLLFWSSGAESQSLLRDGECVMGFLWSTRANVLKSETKGRVAYTFKDGLL
                     QPGIWVVPKGNPAGKEAFRAIASMQRPKEQLYVLEQMGNGPANPAAAGLVPESLRADN
                     PSDPANAAVQAKMGADWYIKNHSKVFQAFMGRIAS"
     gene            complement(17832..18851)
                     /locus_tag="VGC69_11105"
     CDS             complement(17832..18851)
                     /locus_tag="VGC69_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295869.1"
                     /translation="MTASNSHKTQSRGETLGIQAVSKRYGAFQALDGVSLDVRQGEFL
                     TLLGPSGSGKTTLLMSIAGFIQPDAGRLTLGGRDITRAAPEKRNFGMVFQGYALFPNM
                     NVSENVRFPLSMRAEKGAQADKKVAGALELVGLADKGKRYPRELSGGQQQRVALARAI
                     VFQPHLLLLDEPLSALDTQLRGQLQSELKRLHRTVGLTFIYVTHDQGEALSMSDRIAV
                     MKDGRIEQTGTPQALYDRPGNRFVAEFLGIENFLPPALTGQPGTLALRADALRLASGA
                     GRIRARVVERGYHGAEIRYTALADGGQALRFNLHLQAAARVPQENEEIALDWRDADAL
                     PVGSE"
     gene            19021..19686
                     /locus_tag="VGC69_11110"
     CDS             19021..19686
                     /locus_tag="VGC69_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0295870.1"
                     /translation="MKREAMIQAARELFEARGFDSTQMEEIAASAGVSTATAYNYFES
                     KTNVLASIALLHLRSALPARRALLRKLPADPVKGAAAFERLLAKQALATMGENGWRVI
                     FRAGYDMPPTKLSRLGKLMSRKITQHYLKMLRAYRDQGSLVPDTDVELAAELLTTVGM
                     ARFARFVMDDEMSAKERLAVIPRQVSVILSPWLPATRPARSPRAADRPPPPARRRAAG
                     ARP"
     gene            complement(19604..21151)
                     /locus_tag="VGC69_11115"
     CDS             complement(19604..21151)
                     /locus_tag="VGC69_11115"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0295871.1"
                     /translation="MSHSQPARPRASLYGRRHAVVTGHHLASRAAAARLEQGGSAVDA
                     MIAASAVLTVTVPHATSLGGCAMLLEYDAAEGRLRALNGSGRAPAAARPDLFPRGLDP
                     RGPRAWVVPGLVRLWAQAHRSRGRLPWPSLFTDAIDLAEHGTGFGSEVARNLARASAD
                     VRAQPGFAQAFSADGRDRAAGSLHTQPALAWTLKHIAQGGEDAFYRGEVAWRLAAYAR
                     QVGALLSAEDLAASSAEWTEPVMQDYGRWQVGVMPPNSVGVLMLAQLERLKPVYDLPR
                     DERLHAQILHAKDCLPRLRDRVADPQAAWPWQDAAAAVSPAKSAADSVDPRRGDPGDT
                     AGIVLADAEGNGLVMLQSVFQPFGSGCVDPGTGILMNNRLGEFSLKPGAHNLLAPGKR
                     PVHTLNPYIVLADGRPVLYAVSPGGVSQTTTGVQCIFNALLDGMRLPQAVDAPRWSLS
                     RDGEVMCEPGFPDEVVARLREAGLAVATDSLHPFYFGSIKAVRRLPGGVLEAAADLRR
                     EANALAW"
     gene            complement(21178..21669)
                     /locus_tag="VGC69_11120"
     CDS             complement(21178..21669)
                     /locus_tag="VGC69_11120"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0295872.1"
                     /translation="MSLEIFKTLKGKTPEERLAELGIELPPKRTPMGNYCGAVSTASK
                     LVFVAGHGTFNGPTQTHKGKLGEISVEEGKAGARQAIISALTSLKAEIGELSRVKRIV
                     RLFGMVASTPDFHRQPEVIDGASDVLTAVWGPAGVHARCAVGFAALPADMPVELEMVV
                     EIE"
     gene            complement(21702..22790)
                     /locus_tag="VGC69_11125"
     CDS             complement(21702..22790)
                     /locus_tag="VGC69_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295873.1"
                     /translation="MKHSQPGIVGVACVPHAPQFLSRPDSEDLAQVDRVHAAMRQLGE
                     RLRALEPDCIIMLSNDHGDHFVTHTVPPFCVHAAPQADGMHKHRGNWTLDAGMAYPLV
                     QCMGEEGFDLAFTLDAKLPTAFTIPYEFMGFSRDVPLTAIFVNAYVPPQPSPLRCHAF
                     GQALARAVARLGKRAVLIASGGLSHYPGTAHYPHPDLETDKVIFEQMSAGNLTHLLTY
                     DSAALDKTGNVELRMTLAAAAVAGNRKPFMALFEPSWHHIYSVHAWDLTEPASPAPLI
                     YPALPQHHAALVAAVFRLRTDPAGARAFLADPAAFCDGYALAAGERDALMAVHATLDT
                     DRLRDEFSIHALLTAGAATQLRLQRDKA"
     gene            23099..23818
                     /locus_tag="VGC69_11130"
     CDS             23099..23818
                     /locus_tag="VGC69_11130"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0295874.1"
                     /translation="MTALDEHVDPGTPENGEGSGRLRRPAGVYEALFQEIVSGKLRAG
                     ERLLIDDLAARFGVSKIPVREALKALEGKGWVESAPRRGTYVRRLSREELHELFELRL
                     IVEPEITAMAAHRRRASHLQELAAFIEEGEAAVQVSDFVTSSRINSQFHAVIAQAAGN
                     SLAYAVIQDLELRLCRYFFVAEWEERRESILQHRSLYEAIRDQDVEAARKVTIAHLRH
                     TEAMASRSLLVRRMAGLEEDK"
     gene            complement(24030..24293)
                     /gene="rpsT"
                     /locus_tag="VGC69_11135"
     CDS             complement(24030..24293)
                     /gene="rpsT"
                     /locus_tag="VGC69_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY0295875.1"
                     /translation="MANTAQARKRARQSVERNKHNSSLRSMLRTAIKRVRQAIDAGDK
                     AVASETLQKATGVIDRVADKNIIHKNKAARHKSRLAAAVKALA"
     gene            complement(24417..25400)
                     /locus_tag="VGC69_11140"
     CDS             complement(24417..25400)
                     /locus_tag="VGC69_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0295876.1"
                     /translation="MNLSWQPLSDQVVNWLVLLRSGRATPADHASFVAWRAADPRNES
                     AWQQLNDTLRSSGETGRLSDAYPYANPETHDKPATRGVGQGLNRRHFLGGLLALGGAT
                     VAGAAAVNAYYPLAGLGNDMATQTGERRLYRLSDGSQMLLDARTRINLSYTASERTLQ
                     LVAGALSLTAQPDPGRPLLIRTGEGLVQALGARVVVRQQARRTLVIAPDNEVEIETSS
                     GTRQIIPAGTGTRFDNTRIGEPRADLLADAAWENGWIEARDRPLAEVITALRPYREGV
                     LRISMAAGGLLARGRYPLDDTNAALRALEADMPIQVHRITPWFTAIDVASV"
     gene            complement(25786..27354)
                     /gene="murJ"
                     /locus_tag="VGC69_11145"
     CDS             complement(25786..27354)
                     /gene="murJ"
                     /locus_tag="VGC69_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY0295877.1"
                     /translation="MALFKSAATISGLTLLSRITGLARDILIARTFGASPLTDAFWVA
                     FRIPNLLRRLFAEGAFSQAFVPILGTARAQHGDDGIRPLLDRVALLLTIVLALLTVVG
                     IIAAPWVVTLMASGLRDTGRGHEFGAAVAMTRIMFPYILCMSLAAFATGVLNTWRRFA
                     LPALTPVLLNLCMIAACLWLSPRMGLPIYGLAIGVMAGGVAQLAVQWAELAHIGLAPR
                     LLSTRLTQAWHDATVQRIIRQMAPATLGVSVAQISLLINTNVATWLRPGSVTWLSFAD
                     RLMEFPTALLGVALGTVLLPSLSAAHAKNDHDTYSNLIDGGLRLTLLLGLPAAIGMAL
                     LSDGLVATLFNYGAFHAEDVRQTHLAVIAYSVGLIGLLAVKILAPGFYARQDIRTPVK
                     IAIAVLVLTQGFNLLFVPIFAHAGLALSIGLGACVNAGTLLIGLRRRGVYRPAPGWGR
                     FFLRMLPALAGMGVLLWYAEHRLDWIALQTHAGQRALWLAGVLAASAAVYFGLLFATG
                     FRPADFDHRGVGGR"
     gene            complement(27559..28317)
                     /locus_tag="VGC69_11150"
     CDS             complement(27559..28317)
                     /locus_tag="VGC69_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY0295878.1"
                     /translation="MEIANKVFIVTGGASGLGAGTVRMLTANGAKVVIADLQDEPGQA
                     LAKELGQYYVHCDVTQEADAQAVVAQAKSLGPVFGLVNCAGIAPASKIVGKDGAHPLA
                     LFQKVVSINLIGSFNMMRLAAEAMSANTPEPTGERGVLINTASVAAFDGQIGQAAYAA
                     SKAGVVGMTLPIARDLARTGIRCMTIAPGIFGTPMIFGMPQEVQDSLAAAIPFPSRLG
                     RPEDYAKLVYSIVTNEMLNGETIRLDGAIRMPPK"
     gene            complement(28373..29029)
                     /gene="adk"
                     /locus_tag="VGC69_11155"
     CDS             complement(28373..29029)
                     /gene="adk"
                     /locus_tag="VGC69_11155"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY0295879.1"
                     /translation="MRLILLGPPGAGKGTQAAFLTQRYGIPQISTGDMLRAAVKAGTP
                     LGIEAKKVMDAGGLVSDGIIIGLVQDRLKQPDCAAGYLFDGFPRTIPQADALKSAGVK
                     LDYVVEIEVPDSDIITRMSGRRVHPASGRSYHAVFNPPKVEGKDDVTGEPLVQRDDDR
                     EETVRNRLSVYQQQTRPLVDYYSVWSVSEPAAAPKYRKISGVGSVEEITQRLLDALQG
                     "
     gene            complement(29118..29882)
                     /gene="kdsB"
                     /locus_tag="VGC69_11160"
     CDS             complement(29118..29882)
                     /gene="kdsB"
                     /locus_tag="VGC69_11160"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY0295880.1"
                     /translation="MDYTVLIPARAASTRLPDKPLADIAGKPMVVRVAERASQSGARR
                     VCVATDDTRVKAAAEAHGYAALMTRADHATGTDRLAEAVQLLGLPDDAVVVNVQGDEP
                     LIDPALIDAVADCLAASPWADIATCACPLADAEALFNPNAVKVVCAQDGRALYFSRAP
                     IPWARDALAGGDRVLAPGLPAWHHIGLYAYRVSFLRRFPLLQQGLLEKFESLEQLRAM
                     EYGHAIAVHRAAQAPAAGVDTPADLERVRAWYANRL"
     gene            complement(29896..30087)
                     /locus_tag="VGC69_11165"
     CDS             complement(29896..30087)
                     /locus_tag="VGC69_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY0295881.1"
                     /translation="MESRLLDILVCPLCKGRLQFDRAQSELVCRADRLAFPVRDGIPV
                     MLESEARDLDVTTAATPAA"
BASE COUNT         4813 a         9397 c        10943 g         4984 t
ORIGIN      
        1 gctggcgggg ctggtattgc ccgcggcatc cgtcgccgtt gccgtgatgc tgtgcgcgcc
       61 gtcggccagc gccgtgccgg gcgtgaaggt ccagttgccc gaggcgtcgg ccgtggcggt
      121 gccgatcagg gcgccgttgt cgtagatgtt gacggtggcg tgcggttcgg cggtgccggc
      181 cagcgtgggc gtgttgtcgt tggtgctgcc gcccgaagcg accgggccgg tgaccggcgc
      241 ggcgtcgtcg gtcgccgagg tgatggccgg cgcctcgggc gcggtgagat cgggcgccgt
      301 gatcgcggta tcgggcgacg tgccgccggg cgtggtttgc gtgacgtggt ccacttcccc
      361 gtttttctgc gggtcgttca gcggcacgtt gaagtcgccg ttgccatcgg cggtgcctgt
      421 gcccagggac tggcccgacg cgtcggtgat cttgatggtg gcgttgggtt cgcccttgcc
      481 gatgacggcg ctgccgtctc cgttgaagtg cgccgatggg gcggcgggcc gggtgggcgt
      541 gctgttggcg ccgttatcgt cgccgccgcc accaccgccg ccgcccgccg cgatggcgac
      601 gccgccgccg atgaggccca gcgcgccaag caggatggga agggccaggg cggcggcgcc
      661 gccgtccgca tacggcaccc agctgtaagc cacgagaccc gccgtggcgt cgtccggcgc
      721 atagcagacg gcgggattga actccgcctt ccacatctgc tcggtattgt cggcggcgtt
      781 cttgtcgtcc agcaccagcg tggggatggc gtccaccggg cagtcgaagt agccctcgag
      841 gcgcaccgcc gtgccatcgg cgtattggat gagaaggtca tcgccctcgc gctgcatgga
      901 agcgatgtcg ctctgtgtgg cgaacagcac cactttcgct tgatcgtgta tcgggatcga
      961 ggtgttctgc gtgttttgcg caacggtgtg ggtgtctgtc gatccagagc gggcttttac
     1021 atcaatacgc ttgtccatct aggttccctg catgttggtt gatgcttgca cattcagtgg
     1081 gaaatcgatg cgccgtgcga actccattgc gccccatcta ttagtaggta ttttgaaagt
     1141 aagttgagat gtcttttgat aatgaagtgt gtcgacagta aaagacggca atttttatta
     1201 ataaataaga gagcaaattc tttcgacgcc ctgtcttttg gcatacgttg tcgcctgagg
     1261 aggtgaaaga cacgagaaca ggcgtcatgc ctccgtgtca catttgtcta atgtcgaatc
     1321 atcaaaggta tacatgacgc gtacggcacg ttgcgtgttc atcgtggcga tggccatggc
     1381 gcccggactg ggcgccgcga tgccattgga ggaggccgtg cgcctggccg ccttgcgcga
     1441 tcccgtggtc gccgccttgc agcaggacgt ggcgcagcag gaaaccaata tcgagatcat
     1501 cagggacgga cagcgtccgc gcttttccgt gaacgtgggc cgggccacgt cgctcggctc
     1561 ggatccgacg ctggccaccg agcgcaatat cggctcggag ctggtggttt cgcagctgct
     1621 gttcgattgg ggcaaggtat cggcccagat cgaaggcgcg aacgatgacc gcgtgcagat
     1681 cgtgtccgat ctcaaaaagc agctcgagtc ggtcatcttc agcattgccg gactgtatct
     1741 cgacgccgaa accgccaagg ccaaactgca ggcgaccgac gagtaccgca agaacgccgt
     1801 caagctcagc gagatgacgc aggatcgcgc gcagggtgga ttgggcgacg tgtcggagac
     1861 ggcgcgggcg aagctggaga tatcccgcgc ccaggagcgc cagcagactt tcgagtccaa
     1921 tcgcgacgtt gccctggacc aattgaatct gctggtgggg gtttccggca tctcgacgca
     1981 aaaggtgccg gagctggcct acggccagca cgtcggcacc tccgggacgc tggcgcaatc
     2041 catcgagggg gcgccggatt acctcaagtc gcacgcggcc atggattcgg cccggacggg
     2101 catcgatgtg gccaaggcgg ccaccaagcc cgccttgcgc gtggaagcgg cggggcatcc
     2161 ggggttttcc ggcggcggcc gcaccagcgg ttccttggga ctggtgctgg gggtggacct
     2221 gggcgtgtcc gacctgtatg gccgtcaggc ccaggcggcc gagcagaaat atgaaggggc
     2281 ccgcaagcgc ctggaaggcg tcgagcggga cctgaagaac cagacgcaaa gctatgcgag
     2341 gcagctcaag accctggcgg ccagcgaagc cgcgttcagg ctccaggtcg acgaggcgcg
     2401 cagcgtggtg gcgacctatg aagaccagtt caccgcgggc ctgcgcaaca tgacggacct
     2461 gttcaccagc atacaagagc tgtacagcac gcaattgagc tggatcgaca ccatcgacca
     2521 gcttcgccgc acggaataca aggccgcgca ggccctgggc ctgcagggca cgctgctgca
     2581 ggaaagaacc ggcatgtcct tgcccgatgt gccggcgttc ctgtccgcgg agcaggcgcc
     2641 gcagccggtg cggcaaccca cggcgccgtt cgacgatccg ggacactacg ccaaactgcc
     2701 ctttaacagc cgggaggtca cgccatgagc gcggcggatc agatagcgat gtatgaaccc
     2761 ggcctcctgg ccgggctggt gcgattgtgc gcggcgcatg gcgtgagcac gtcggcgcag
     2821 cgattgggcg acggcctgct gcgcgagccg ggcgtgttgc ctttcagcca ggtggagcgg
     2881 gccttgcggc gcgtcaacat gacgtgcggc atggtcgcgc tgccgctcaa gcggctttcg
     2941 gcccatgcct tgccggtttt gttgctgatg cgcgacggca gttgcctggt gctggaaaag
     3001 ctgaccccga cctcggccga agtgtcccat cccgagtcgg gcgcggggcg cagcgccatg
     3061 ccgcgcaagg cgctcgagga gggctatgcg ggccgctacg tcatcgccaa gcacatcgag
     3121 gtggtctccg cgcacctcga tggcagcttc gacaagaagc cccgccactg gatcctgggg
     3181 cccgtgcggg accaatggca tgtctatcgg gatgtcctgc tggggtcgct gatggccaat
     3241 ctgctggccg tcggcgtggc gcttttctcg atgcaggtct atgaccgcgt ggtgcccaat
     3301 gccgcgttcg acacgctgtg ggtgctgtcc agcggcgtga tcgccgccgt cctcctggaa
     3361 tgggtgctgc gctatatgcg ggccaggctg atcgaccata gcggccgcgc gctggacctg
     3421 cagctgtcgg cccagctgtt cgagcgcgtg gtgcacatgc gcttgtcgca ccagccgacg
     3481 tccatcggcg ccttcgccaa ccaggtgcgc gatttcacca gcgtgcgcga tttttttacc
     3541 tcgggcacct tggccacctt gtgcgacctg cctttcgtcg tggtgctgct ggccctgatc
     3601 ggcttcatcg gcggctgggt cgtggcggtg ccgctggtcg cggtggtgtt gatcacgctg
     3661 cccggcatcg tgatgcaaaa gcggctcgcc gcgctgtcgc gccagaacac ccgggaaagc
     3721 gcggcgctca acggcctgct gctggaggcg gtcgataacc tggagaatgt caaagccacc
     3781 cgctcggaag agcgcctgca gcgggcccat gccagcctca ccgccatcat ggctacctcg
     3841 ggcagcaaga cgcgcgatat caccagcatg ctgcagctca tggcgggcag cacccagcag
     3901 ctgtgctata ccgcggtcgt catcgtgggg gtatacctga tcaccgacgg caggctgacg
     3961 acgggcagca tggtggcctg cacgctgctc tcgagccggg tgctctcgcc catgagccag
     4021 gtggccggcc tgctgacgcg ctggcagttc gtcaaggccg ccatggaggg gctggaccgc
     4081 atcatcagcc tgccggtgga acggcctgcc gaccggcatt tcgtgcgcgc ggaacagatg
     4141 cggggcgcct atcggctgga gcacgtggtg tacgcgcaca ggcccgacag ccccgcggcc
     4201 ctgcatatcc cgcgcctggc gatccgggcc ggcgaacacg tggcgctctt gggcggcaac
     4261 ggcgcgggca agtccacgct gatgcgcctg ctggccggcc tgaccgatcc gcagcagggc
     4321 aatgtgctgc tggacgacct ggccctgtcg cagatcgatc ccatcgaccg ccgccggcag
     4381 atcggctacc tgccgcagac cgtggcgctg ttccagggca ccttgcgcga aaacctgacc
     4441 ctgggccatg cgctgtggtc cgacgccgaa ctgctcaagg cgctcgatgc cgtgggcctg
     4501 gggcctttcg tgcgcaagca cgtgcgcggg ctggaccagg acatcctgtc catcggcaat
     4561 atgtcgggcg ggcaaaagca ggccgtcggc ctggcgcgcg tcatcctgca ggaccccagg
     4621 atcgtgatcc tggacgagcc cacgtcggcg ttcgaccagg ccaacgaggc gcgggtggtc
     4681 gggttcatga agacgtggct cgatggccgg accgcgctga tcgccacgca caagcgcgaa
     4741 ctcctggtcc tgacgcagcg ggccatcgtg ctgcgcgacg gcaaggtggt ccacgacggc
     4801 gagatgggcc agctcgtcga gctggccacg cagcgcgcca ggcgcccgcc cgccgcgccc
     4861 gagatcaagg tcgtcacggg aggcgcgtca tgagccgcgg ccacgccatg ctgctcgacg
     4921 tgtcggacgg cggccgcctg cagcgcgtgc ggaccagccg gaccgtgtgg ttcatcacgc
     4981 tcatcctggc gctgctgatc gcctgggcgg cctgggcgca cgtcaacgaa gtaacccgcg
     5041 gcaaaggccg ggtgacgccc tactcccgcc tgcagtcggt ccagagcctg gagggcggca
     5101 tactcaccga actgctggtg cgcgagggcg acatcgtgca ggccgaccag ccgctggccc
     5161 gcatcgacag cacgcggttt cagtcggcct ataacgaaac ccagtcccag atcgacacgc
     5221 tcgcggccga gatcgcccgc ctcgagaccg aggtgaatga aaagaccgaa ctggtcttcg
     5281 agggcgaggg cgacgccaag gcgcgccagg ccgagatgga gctgttcaag gcgcggcgca
     5341 cccggctgga caagtccatc ggcgccttgc aggaagaaga ccgtatcctc cagcgccaga
     5401 ccgccatggt ccagcccttg gtgaagaagc agtcggtgcc cgagtccgag ctcttgcggc
     5461 tgcagcagca gtcggcgcaa ctgcatggcc gtatcggcga actgcgcaac cagtacgtgc
     5521 aggaggccta cagcgacatg gtcgacaaga aggccaagat ggcggcgctg cagcagagca
     5581 tgatgcagaa gaaagaccag ctcgaccgca cgctggtcac cgcgcacgtg gcggggcgcg
     5641 tcaacaatgt gcgcatcaat tcggtgggcg gcgtggtgca gccgggcgag accatcatgg
     5701 agatcagccc catccacgac cagttggtgg tcgagacgct ggtggagccc aaggacgtgg
     5761 ccttcatcgc gcccggcatg ccggccagcg tcaagatcac ggcctacgac tattcggtgt
     5821 acggcgactt gcgcggcaag gtcacgcaga tctccgagga caccgtcaag gaggacacgc
     5881 cgcacggccc gcaggagtac taccgcgtca tggtcaccac cgacagggcc tatctgcaaa
     5941 agggcgagga gcagctgccc atacgcccgg gcatggtggc cgaggtggat atcgaaacgg
     6001 gcagccggtc gatattgaac tacctgaccc ggcccctgat gaaggcgcgc ctgcgctagg
     6061 gcgccgcgcg ccgattattt cttcttgctg tcctgcagct ggggcagggc cgagccgccg
     6121 gagggcagca gcaggccggc cttggtgtag gtgaagagct tgtcgcgcgt gtcggtgatg
     6181 tcgaggttgc gcatggtcag ctggccgatg cggtcggcgg gcgtaaaggg cgcgttctcg
     6241 accttttcca tggacaggcg ctcgggcgcg taggtcaggt tgggcgagac ggtgttcagc
     6301 agcgagtagt cgttgccacg gcgcagctcg atgctgactt cgccggtgat ggcgcgggcc
     6361 acccagcgct gggcggtttc gcgcagcatg atggcctgcg ggtcgaacca gcggccctgg
     6421 tagagcaggc ggcccagctt gcggccgttg tcgcggtact gctcgatggt gtcttcgttg
     6481 tggatgccgg tgaccaggcg ctcgtaggcg atgaacagca gggccatgcc cggggcttcg
     6541 tagatgccgc ggctcttggc ctcgatgatg cggttctcga tctggtcgct catgcccagg
     6601 ccgtggcgcc cgccgatgag gttggcctcg gccatcaggg ccacggggtc gctgtattcg
     6661 acgccgttga gcgccacggg ctggccttcc tcgaagcgca cggtgactgc ttcggccttg
     6721 accgccacgt cgtccttcca gaaggccacg cccatgatgg gcttgacgat gcggatgccg
     6781 gagttcagct gctcgaggtc tttggcttcg tgcgtggcgc cgagcatgtt ggagtcggtg
     6841 gagtaggcct tttccgccga catcttgtag tcgaagccgg cctttgccat gtaggcggac
     6901 atttcggcgc ggccgcctaa ttcgtcaatg aattgttgat ccagccaggg cttgtagatc
     6961 ttgagctcgg ggttggtgag caggccgtag cggtagaagc gctcgatgtc gttgcccttg
     7021 aaggtgctgc cgtcgcccca gatgttgacg ccgtcttcct tcatggcggc caccagcatg
     7081 gtgccggtga cggcgcggcc cagcggggtg gtgttgaagt aggtgatgcc gccggtggag
     7141 atgtggaagg cgccggcctg cagggcggca atgccttcgg caaccagttg ctggcggcag
     7201 tcgaccaggc gggcgttctc ggcgccgtac tgcctggcgc ggcgcgggat gtcttcgtag
     7261 tcgggttcgt cgggctggcc gaggttggcg gtgtaggcgt agggaatggc gcctttctcg
     7321 cgcatccaga tcagggccgc gctggtgtcc aggccaccgg agaaggcgat gccgactttc
     7381 tggccgacgg ggagggaggg gagaatggtt gccattgtgc gaagggcgcc aacacgcaag
     7441 tgcgcgccaa gtaagggtag gaaaaggtca aacgcgcatt ttaacgcgtt tgccaggcgt
     7501 gccggccccg gcggcggcgc ccacgaacag ccgcttcagg tcgagttccc gcagcagttg
     7561 cgcggtggcg ctggccagcc gcggggcggg ccggtggcgc ggcacggcca gcaccagatc
     7621 gttgcggatc acgggcgctt cgatgcggca caggaccagg ggatgggcgg cagccggccc
     7681 ctgcgccgcc gagcgcggca ggatggcgta ggcatcgccc tgcgaggcga gttcgacgat
     7741 ggtctgcacc gcgtcgacct cggccgccac ggtcaggcgc acgccctggc gccggcacgc
     7801 gctttcgacc agggcgcgga tggcgttggg caggctgggc aggatcaggg ggtaccggcc
     7861 cagttgggcc acttgcacca cggcgggcag cggcaggcgc ccgcctggcg ccgccgccag
     7921 caccaggtct tcccggaaca gcgattcgta ggccaattgc ggcgaggcgg gcgggtcgta
     7981 cagcagggcc aggtcgatgc gcccggccag cagccattcg cgcacctggg tgctcagacc
     8041 ctcggccacg gtaatcgagg catcggggca ggcgcgccgg aaagcctgca ccagcggcgg
     8101 ggtgacgggg cgggcgatgc gcggcggcag gccgatggcg accttgccgg tgggcgtgtc
     8161 gcgcagtccc tgcaggtctt ccttggcccg ggcggccagg gccagcaggg cgcgagcata
     8221 ttccaggaag cgcaggcccg cctcggtggg ttcgaccccg cggccgttgc ggtagagcag
     8281 gtgctggccc agttcctgtt cgagcaggcc cacctggcgg ctgagcgtgg gctgggcgac
     8341 gtccagcagt tcggccgcgc gcgagaagct gcggcgctcc gccacgcgca cgaagtattc
     8401 gatctgcttg atgtccatat gggatatttg atttttctat atctgaaatc atagatatat
     8461 cattgttcgg gcgcgggcga tcggccacca taccaatggc cggcgcgcgc gatgcgcggc
     8521 cggcccagaa caataatgac cggagaccgc ttcgtgcccg attgccgccc gccgctcgcc
     8581 gcgccctcgc cgccgcgccg ccgcctgccc gagggggcat gcgatacgca ctgccacgtg
     8641 ttcggcccgg ccgatcgctt cccctacgcc gaaggccgct cgtacacgcc gcccgacgcg
     8701 ccgttcgaga aactggcggc cttgcacgcg cacctgggcg ccgcgcacgc ggtcatcgtg
     8761 caggccaatt gccacggcca tgaccatgcg gccttgctgg acgcggtggc gcgcagccag
     8821 ggacgctacc ggggcgtggc gctgctgccg ccgcaaaccg acgcggcgat gatccgggcg
     8881 ctgcatgccg ggggcgtgcg cggcgcccgt ttcaatttcg tgccgcacct gggcgcgccg
     8941 ccgtcgcccg aggcgttcga ccgcatggtg tcgctgatcg cgccgtttgg gtggcatgtg
     9001 tgcctgcacg tcgacggcgc catgatcggc gagctgctgc cgcggctggc cgggttgccg
     9061 gtgccgttcg tggtggatca catggggcgc attcgcgctt gcgacggact ggacgcgccg
     9121 gccttccggg cgctgctggc gctggcgcgc gtgccgggcg cctgggtcaa ggtgtcgggc
     9181 atagaccgca tcggcgacgg ccggcggcct tttcacgaag gcattccctt catccgcgcc
     9241 ctggtcgagg ccatgcccga ccgcactgtg tggggcaccg actggccgca tccgaacgtc
     9301 gccggcgaca tgcccgacga tggcgaagtg gtcgatgcct ttttcgaggc ctgcccggac
     9361 gaggcggcgc gacggcgtgt gctggtggaa aatccggcgc ggctgtatgg gttttagatc
     9421 gtccgggccc cggtccgggc gcggcgcggc agaaggcagc ggacggcaaa acgccggccg
     9481 ccatgaagaa aacaagagga gacgcaaatg ttcaaacgca tggtgtgcgc gggcgcgctg
     9541 gcctgggcgg cgctgggcgc ggcgcaagcc gaagggccgg tgcagctcat cgtggctttt
     9601 ccgcccggcg gcccggtgga cctggtcgga cgcgtgctgg ccgaacaact gagcaaggca
     9661 ctggaccgcc cggtcatcgt cgagaacaag gcgggggcca acggcaatat cggcgcggcc
     9721 tatgtgtcca aggccccggg cgacggctcg gtcctgttcc tgaccagcgt gggggcggtg
     9781 tcgatcagcc cggcgctgta caagtcgctg ccctacgacc cggtcagcgg cttcaagccg
     9841 atttccaagg ttgtcgacaa cgccacggtg ttcgtcgtga atccctcgaa tcccgccgtc
     9901 gatgccgcgg atttcgtcaa gcgttcgcag gcggccagcc agccggtggc catcggttcg
     9961 tcgggcgtgg gcagcattcc ccacctgacc ctggagatgt tcgccgacgc cagcaaggcc
    10021 aaggtgatgc atgtgccgta caaaggggcg gcgccggtca tcagcgatct ccttggcaat
    10081 caggtggccg gcttcttcgg cgacgtgccg ggcctgatcg gccagataca gagcggacgc
    10141 ctcaaggtgc tgggcctggc cgcgcccgag cgcctgccgc tgctgccgca ggcgcggacg
    10201 ctggccgaac agggcatccc gggcgtcgag accaataatt ggtacggact gctggcgccg
    10261 gcggccacgc cgcccgcgct ggtcgacaag ctcaacgccg ccgtgcgggc cgccttgcag
    10321 gctcccgagg tgcgcgacaa gctggcgcag ttcggctgcc aggtcgcccc caccagcccg
    10381 caggaactgg ccgacctgat cgcggccgac cgccgcaagt ggtcggacct gataatgcac
    10441 aagaacatcc acctggagta atggcatgag cccataccgc attgccccgg tcgagccggg
    10501 cgcgcgcccg gaactggccg gcatcgaggc acgcatcctg gccgcgcgcg gccgcatcac
    10561 gccgctgtac caggtgctgc tgaacagtcc gccggtggtc gacggctggg aggccatgct
    10621 gacggcgatc cgccagaaaa ccgcattgtc gccgcgcgtg cgcgagctga tcatcctgcg
    10681 cgtggcggtg ctcaaccgcg ccccgtacga attcgaggcc catgtgccgc acgcgcgcgc
    10741 cgccggcctg cgcgaggcgg tcatcgaggg cctgcgggtc tatgccgcgc cggacgcagt
    10801 ccaggggctg gacggcggcg aacgcgaagt gctggccctg accgatgcca tgacgcgcga
    10861 catcgaggtg ccggacgccg tgttcgagcc cttgcgcgcg cattttgacg agcagggcct
    10921 gatcgagctg gccgcgaccg tggcggccta caacatggtg tcgcgctttc tggtggcgct
    10981 gcgcatcggg cactgagcgg tccgggagga atcatcatgc agacacagga aatattgctg
    11041 gtgaaggtgc cggcgaggct ggacgccgcg gccgccggcc gggtccgcgt cgcgctgtcc
    11101 gacggcctgg cgctgtcggt ggaggtgtgc gccgcgctcg aactcgacga gtcgtacgtg
    11161 tattgccggc cgcgtagcgg ggtcgacgcc gcgcaggcgc tgccggcagc ccgcggccgc
    11221 gcggcgaccg cctgtccggc gggcagccag gccctgctgc tggagggcct gctgcatttg
    11281 caaagcgagc cggacggcgg cgccgcgccc tggcactaca tcgtcgagac cgacgtcacg
    11341 cccgagttcg agcgcgagat gaacgattgg tacgatcggg agcacatgcc cggcctggcc
    11401 gtcgtgcctg gcacggtgcg ggccgatcgc tacctgtccc gggacggatc gccgcgctat
    11461 tacgcctgct acgacctgcg caccctcgag acgttcggca gtcctccctg gctggccgtg
    11521 cgcgcgacgg attggagcag ccgcgtccgc ccggcgttcc gcaataccaa gcgcaccatg
    11581 ttcgcgcggg tgtagcaagg agacgagcat gcgcatcaaa ccttggctgg cttgcctggc
    11641 ggccggtgct gtctttgtcc tgggggcggc gcggccggcc gatgccgcgg ccacggcggc
    11701 cgtggccgcc gatgcggtcg aacatcatta tgtgttcgcc ggcggcggcg tgcgtatcga
    11761 agtgctggcg cagggcagcg gccgcccgct ggtgctgctg ccctcccgcg ggcgcggcgc
    11821 gcaggatttc gatcaattgg cgcagcgttt cgcgcaagcg ggctatcgcg tgctgcgccc
    11881 gcagccccgc ggcatcggcg gcagcatcgg gccgatgcga ggcgcgacct tgcacgacta
    11941 cgccgacgac atggcggcgg tgatcgagga tgtcgcccgc cagcccgtcg tcatggtggg
    12001 ccacgccttc ggcaattggg tcgcgcgcgc cacggccgtc gatcatccgg aatgggtgcg
    12061 cggcgtggtc atcgtggcgg ccgcggccaa gcagtatccg cccggattga gccagctggt
    12121 ggatcgttcg agcgacctgt cgctgccgga cgccgagcgc ctgaaggcca tccaggacgc
    12181 tttcttcgcg ccggggcacg acgcccgggt atggctgggc ggctggtatc ccgaggtcaa
    12241 cgaaagccag aaccaggccg gcaaggcgac ccggcaggcg gactggtggt cggggggcaa
    12301 ggcgcccatg ctcgacctgc aggccgatct ggacaagttc aagccgcccg ccacgcgcgg
    12361 cgaggtgcgc gacgaattcg gccagcgggt gacggtggcg gtgatccgcg gcgcgggcca
    12421 cgcgctggtg ccggaggcgc cccaggccgt ggccgatgcc gtcatcggct gggaacgcgg
    12481 cctgccggac cggccttgag caggtttaca gctgccccag cagcaggaac tccatcagcg
    12541 ctttctggac gtgcaggcgg ttttccgcct cgtcccacac cacgctctgc ggcccttcga
    12601 ggacctcggc ggtgacttcc tcgccgcggt gggcgggcag gcagtgctgg aagatggccc
    12661 gcgggtcggc cacggccatc atgtcggcgt ccacgcacca gtcggcgaaa gccttgcggc
    12721 gggcctcgtt ctcggcctcg tagcccatgc tggtccagac gtcggtggtc accaggtggg
    12781 cgccgaggca ggcgtccatc gggtccttga actgcctgag caccgtgggc gggggcgcgc
    12841 cgatgcgcgc cgggtcgagc tcatagcccg cgggcgcgca gacgtgcagg gtgaagccca
    12901 gcagctcggc ggcctgcagc caggtatagg ccatgttgtt ggagtcgccc acccaggcca
    12961 ccgtcttgcc ggcgatgggg ccgcggtgct cgatgaaggt gaggatgtcg gccaggatct
    13021 ggcaggggtg gaattcgttg gtcaggccgt tgatgacggg cacccgcgaa tacgcggcga
    13081 agctttcgat gcgcgcctgc tcgaaggtgc ggatcatcac gatgtcgacc atgcgcgaga
    13141 tgatgcgcgc ggtgtcctcg atgggctcgg agcggcccag ctgggagtcg ttggaagtca
    13201 ggttgatgac cgagccgccc atctggtaca tgccggcctc gaacgagacg cgggtgcgcg
    13261 tgctggcctt ctcgaacacc atggccagcg tgcggtcgtg cagcggcatg tggggttcgt
    13321 agcgcttgaa cttgtccttg atgacgcggg cgcgatccag cacgtaggcc aactcgccgg
    13381 acgagaagtc cttgagctgc aggaagtgcc gcagcggggc cgggttgccc tgggcggcac
    13441 tcggatttac gttggacggg gacggggtcg cggtcatggt taaaaatcac aaagccaggt
    13501 tggcgaaaag gtcaatcata tacaattcgg gcgcgggtcg aattgcgacc cccgggccga
    13561 cccggcctgg gtggcgggca aacgccggct ccgcagtgaa gaaacatgac tacgcgcgtt
    13621 cgacaattcc tcatctacgg tattacagaa agcggcagcc gtttccgtcc cagcgactgg
    13681 gcagagcgcc ttgccggtgt catggcgcag ttccgtcccc ccggcgtggt cggcacccac
    13741 ctcacgtatt cgccctatgt gctgcccgtg gtcatggacg gggtgcgcgg cgtcgtggtc
    13801 gaccagcggc tgcgtgaact cgaaccgctg gcctacaagt tcgtggtgga tttcgcccgc
    13861 gacaacaacc tcaagaccga agagcgcgag atcgacgaaa aggcctgaac gcgctcagac
    13921 gctgtcgcgg atcagcggcg cggtcgagca gtgcaggccg ccgccgccca gggcgacatt
    13981 ggcgtagggc acgacgcgga tgctgacgcc ggcgcgctcg aacttctcgg cggtgcgatc
    14041 cgacaggcct tcggccatca gcacgcggcc cggccggacg gccaggcagt tcacgaccca
    14101 gttctcgtct tcgtcgcaga cctcgatggt cctgatcttc agttcctgga gtttttccag
    14161 gaaccagaag ggcagcagcg ccggattgat gaaagcggtg tcgacgtcga ccatgacgag
    14221 catgccgtcg atgtgcagcc ggtagcccgt cagttcgatg gtcagcagct cgacgccttg
    14281 cgtggccagc acctcgcgca gttgccgggc gccttcggcg ttgacgcggc tggataggcc
    14341 gagcacggcg gtcttggcat tgagcagcac gaagctgccg ccctcgagca cgccggtgcc
    14401 cgcgatggtg cgcaggatgg gcatgcccag cgcggccagg gtgcgggtga cgggcagctc
    14461 ttcgccgcgg cggatcgggg gccccaggcg ggtgacgatg gcgccgccat tgacggcgat
    14521 cacgctgtcg cgggtgtaga cctgcttcat gcggccgggc gcgacctggt cgatgtagac
    14581 gacttcggcg ccttcctcgc gcaggactgc ggcataggcg tcatgctcgg cctgcatcgc
    14641 cgccagcgag gggatgacgt cgccgcgcca gtaccagccc ttgtccactt cgccgaaggc
    14701 attgatgccc gggatgcgct tgccggggtc gatgactttc aattcgtcgc cggggcggtg
    14761 catcagcacc ttgcggatct ggccgacgtc gttgtcgcag ccccagaccc gcccccagtt
    14821 ggcgatctgc tcttcgggcg tgtggaaggc gggttccgcc ttggagggaa actgcttgat
    14881 cagcatgttg tagcggtatt cggcttcggt aatgatgccc atcggggtct ccgtagaggg
    14941 tgtttgaaaa aggtaggctc agtgtttctt cctggcgcgc agcgccaggt cgagcgtgaa
    15001 gagggcgagg gtgagcagga tgaggacgga ggcgaccgcg gccatggtcg ggtcgagcgc
    15061 ctcgttgatg ccgtcccaca tcttgcgcgg cagggtgaag acggtgcgcc cggcgatgaa
    15121 cagcacgatg accagctcgt cccaggagtg gatgaaggcg aagaccagcc ccgagacgat
    15181 ccccggccgg atgttgggca ggatcacgtg gcgcagggtc tggccgatgc cggcgcccag
    15241 gttgcgcgcg gcctgctcca gccgcgggtc gagcccggcg agcgcggtcg acaccgtgac
    15301 gatcacataa ggaatgccgg tgacggagtg ggcgacgatg atgccggtat aggtgttgat
    15361 ccagcccagg tcggaccaga cctggtaggc ccccagggcg tagatgatgg gcggcagcat
    15421 catcgggacg agcatggcga agcgcaccat gttggccagc gcggtgccga tgcgccagca
    15481 gccgacggcg gccagcgtgc ccagcgtgcc ggcgatggcg gtcgaggcgc aggcgatcac
    15541 gaagctctgc cagatcgacg cgagccactg cggcgaggtg aagagcttgt ggtagttctg
    15601 cagggacacg tgttcggccg gcagcgacag gtagctgcgg tcggtcagcg agacggcgaa
    15661 caggatggtg atgggcagga cgagaaagca gatgatggtc caggcgagcg agcgggtcgc
    15721 catggcgggt ttttgctgaa aagccgagga catgtcagcc tccgcacggc cgcccgacgg
    15781 aggctgaagc cccctcgggg ggcagagaac gtagtgagcg tgggggtctc attcaagcgg
    15841 ctccgaacag tttgcgcagg tcgatgaagc ggccgaccgc ggccagcacg gcgaggatga
    15901 agatcagcag caggctggcc agggccgagc ccacgcccca gttcacgacg tcgaggatct
    15961 gcatcttgat gtattcggcg atcatcaggg tcttgccgcc gcccagcagc gccggggtga
    16021 catagaagcc gagcgcgtag atgaagacga gcatcgaggc gccgaagacc ccgggcaggg
    16081 tcagcgggaa atacacccgc cagaaggcgc gccggcggct cgcgcccagg ccgcgcgcgg
    16141 cggcgaccag gcgcgggtcg atggcgtgca tgccggaata cagcggcagg atggccagcg
    16201 gcagcatgta gtgcaccatg cccaccagca cggcgaactc gttccacatc agcggcaggg
    16261 gcgaactgat aatgcccagg cccatcagca gctggttgac gaggccgtcg ttgcgcagca
    16321 gggtgaacca ggcgaaggcg cgcaccagca ccgagatcca caaggtcacg accacgccca
    16381 tgaacagcac gcgccgccgc cgctcgtcgg cgttggcgat gacgtaggcc acgacatagg
    16441 ccagcatcag cgtggcgacg gtggaaagca gcgtgatccg cagcgtggtg ccgagcatgc
    16501 ggtggatgga gcgcgacgcg gccacgaacg cgaagttcga caggccgggc tcgggcaccg
    16561 tcacgctcat ccagagcacg cggccgatcg ggtagaagta gaaagccgcc aagcccagga
    16621 gggcgggcag ggtcagcagc cagtacttga gcaggggatg gcggaatctc ggcacgttca
    16681 tcgatcagga ggcgatgcgt cccatgaacg cctggaagac cttggagtgg ttcttgatgt
    16741 accagtcggc gcccatcttg gcctgcacgg cggcattggc cggatcgctc gggttgtcgg
    16801 cgcgcagcga ttcggggacc aggcccgcgg cggcggggtt ggccgggccg ttgcccatct
    16861 gctccaggac gtagagctgc tccttggggc gctgcatcga cgcgatggcg cggaaggctt
    16921 ccttgccggc cgggttgccc ttgggcacca cccagatgcc cggctgcagc aggccgtcct
    16981 tgaaggtata ggcgacgcgg cccttggtct ccgacttcag cacgttggcg cgcgtcgacc
    17041 acaaaaagcc catgacgcac tcgccgtcgc gcagcaggct ctggctttcc gcgcccgagg
    17101 accagaacag caggctgtcc ttgatgctct cgaacttggc catcgcgcgc tcgacgtcca
    17161 tcgggtagag gtccttgatg gccacgccgt cggccagcag cgccatttcc agcatggcct
    17221 ggctgtcctt gcggatcatg cggcggccgg ggatcttctt gatatcgaag aaatcggcca
    17281 gcgtcggcgt gccctggatc ttggcggtat cccaggccat gaccgtcgag aagaagtagt
    17341 tcaccacgcc cagctcgtag gcgaagccgt ccatcacctt ggacttgtcg acgatgctgt
    17401 agtcgatcgg ctccagcgcg cccttgggac cgagctcggc gatggtggtg atgccgccgt
    17461 cgcagatgtc ccaggtgaca ttcttggact gcaccatggt caggatcttg ccgttgaccg
    17521 ggcccgagcc gtcgatcacc atcttgccgc ctgcggcgac gtacgggtcg gcgaaggcgg
    17581 tcttgaagac gtccacggac accccgcccc agttgaccag cacgacctcg ggcttgtcgg
    17641 cggcgaaggc attgcgcgcg cccatggcgc ccacggcgag cggcgcgacg cccattgcca
    17701 gggcgctgcg caggaggtcg cggcggctca tgccgcgggc gcgctgctcc tgcatgagct
    17761 cggcatagtc ttgggcgaaa gtctgttctt ccatgatgtg ttcccctgtg cgatggtttt
    17821 tggactgcct gttactcgga cccgaccggc agtgcgtcgg cgtcgcgcca gtcgagcgcg
    17881 atttcttcgt tttcctgcgg cacgcgcgcg gcggcctgca ggtgcaggtt gaagcgcagc
    17941 gcctgcccac cgtcggccag cgcggtgtag cggatctcgg cgccgtggta gccgcgctcg
    18001 accacccgcg cccggatgcg gccggcgccg ctggccaggc gcagggcgtc ggcccgcagc
    18061 gccagcgtgc ccggctgccc ggtgagcgcc ggaggcagaa agttctcgat gccgaggaac
    18121 tcggccacga agcggttgcc gggcctgtcg tacagcgcct ggggcgtgcc ggtctgctcg
    18181 atccggccgt ccttcatcac cgcgatccgg tcggacatgg atagcgcctc cccctggtcg
    18241 tgggtgacgt agatgaaggt cagccccacg gtgcggtgca gccgtttcag ctcgctttgc
    18301 agctggccgc gcaactgcgt gtcgagcgcg ctgagcggtt cgtcgagcag gagcaggtgc
    18361 ggctggaaca cgatggcgcg ggccagggcc acgcgctgct gctggccgcc ggacaactcg
    18421 cgcggatagc gcttgccctt gtcggccagg ccgaccagct ccagggcccc ggcgactttc
    18481 ttgtcggcct gcgcgccttt ctctgcgcgc atcgacagcg ggaagcgcac gttttcgctg
    18541 acgttcatgt tgggaaacag cgcatacccc tggaacacca tgccgaagtt gcgcttttcc
    18601 ggcgcagcgc gggtgatgtc gcgcccgccc agcgtcaggc gcccggcgtc gggctggatg
    18661 aaccccgcga tcgacatcag cagggtggtc ttgccggagc cggacggccc caggagcgtc
    18721 aggaattcac cctggcggac gtccagcgag acgccatcca gcgcctggaa cgcgccgtag
    18781 cgcttggaga ctgcctggat gccgagcgtt tcgcccctgg attgcgtctt gtgcgagttg
    18841 cttgccgtca tgggccgcta taaatttata gtcatatata attttattgt tgactctata
    18901 tttataggtg tctataaggt tttccctggg gcagaagcgg acgggggtag aatcgccttg
    18961 cgtacacgca ggcaataggg cgtcattcat tgcatgggat tgagaacacg aaacaagcgc
    19021 ttgaagcgcg aagcgatgat ccaggcggct cgcgagctgt tcgaggcgcg cggcttcgac
    19081 tccacccaga tggaagaaat cgcggcgtcg gcgggggttt ccaccgccac ggcctacaac
    19141 tatttcgaaa gcaagaccaa tgtcttggcc agcatcgcgc tcctgcatct gcgcagcgcg
    19201 ctgccggcgc ggcgggcgct gctgcgcaaa ttgcctgccg acccggtgaa gggggcggcg
    19261 gctttcgaac ggttgctggc gaagcaggcg cttgccacca tgggcgagaa cggctggcgc
    19321 gtcattttcc gggccggcta cgacatgccg cccaccaagc tttcgcgctt gggcaagctg
    19381 atgtcgcgca agattaccca gcactacctc aagatgcttc gcgcttaccg cgatcagggc
    19441 agcctggttc ccgacacgga cgtagaactg gcggcggaac tcttgacgac agtgggcatg
    19501 gcccgtttcg cgcggttcgt catggacgac gagatgagcg caaaagagcg gcttgccgtc
    19561 attccgcgcc aggtgagcgt catcctgtcg ccctggctgc ctgctaccag gccagcgcgt
    19621 tcgcctcgcg ccgcagatcg gccgccgcct ccagcacgcc gccgggcagc cggcgcacgg
    19681 ccttgatcga cccgaaatag aaggggtgca gcgaatctgt cgcgacggcc aggccggcct
    19741 cgcgcagccg cgccacgacc tcgtccggaa agcccggctc gcacatcact tcgccgtcgc
    19801 gcgacaggct ccagcgcggc gcgtcgacgg cctgcggcag gcgcatgccg tcgagcaggg
    19861 cgttgaagat gcattgcacg ccggtggtcg tctggctgac gccgccgggc gagacggcat
    19921 acaggaccgg cctgccgtcg gccagcacga tgtaggggtt gagcgtgtgc accggccgtt
    19981 tgccgggcgc gagcaggttg tgcgcgccgg gcttgaggct gaactcgccc aggcggttgt
    20041 tcatgaggat gcccgtgccc gggtcgacgc agccgctgcc gaagggttgg aacacgctct
    20101 gcagcatgac caggccattg ccctcggcat cggccagcac gatgccggcg gtgtcgcccg
    20161 gatcgccgcg gcgcgggtcg acggagtcgg cggcggattt tgccggcgag acggcggcgg
    20221 ctgcgtcctg ccatggccag gcggcctgcg ggtcggccac gcggtcgcgc agcctgggca
    20281 ggcagtcctt ggcgtgcagg atctgggcat ggagccgctc gtcgcgcggc aggtcgtaga
    20341 ccggcttgag ccgttccagc tgcgccagca tgagcacgcc caccgagttg ggcggcatga
    20401 cgcccacctg ccagcggcca tagtcctgca tgacgggttc ggtccattcg gcgctgctgg
    20461 cggcgaggtc ctcggcgctc agcaaggcgc cgacctgtct ggcgtaggcg gccaggcgcc
    20521 atgcgacctc gccccggtag aaggcgtctt caccaccttg cgcgatgtgc ttgagcgtcc
    20581 aggccagcgc gggctgcgtg tgcagcgaac cggcggcgcg gtcccggccg tcggcgctga
    20641 aggcctgcgc gaaaccgggt tgcgcgcgca cgtcggccga ggcccgcgcc aggttgcggg
    20701 cgacctcgct gccgaagccc gtgccgtgct cggccaggtc gatggcgtcg gtgaacagcg
    20761 agggccaggg caggcggccg cggctgcggt gggcctgggc ccacagccgc accaggccgg
    20821 gcacgaccca ggcgcgcggg ccgcgcgggt ccagcccccg cgggaacagg tcgggccggg
    20881 ccgcggccgg ggcccggccc gaaccattga gggcgcgcag cctgccctcg gccgcatcgt
    20941 attcgagcag catggcgcag ccgcccagcg acgtggcgtg cggcacggtg acggtgagca
    21001 ccgcggaggc ggcgatcatg gcgtcgaccg ccgagccgcc ttgctccagg cgcgccgccg
    21061 cggcgcggct ggccaggtgg tggccggtga cgacggcgtg gcgccggccg tagagactgg
    21121 cgcgcggccg cgcgggctgg ctgtggctca tgggttggcg cgggtgattg cggggcgtta
    21181 ttcgatttcg acgaccattt ccagctcgac cggcatatcg gccggcaggg cggcaaagcc
    21241 cacggcgcag cgcgcgtgca cgccggccgg accccacacg gcggtcagca cgtcggaggc
    21301 gccgtcgatc acttcgggtt ggcgatggaa gtcgggcgtg gaagccacca tgccgaacag
    21361 gcggacgatg cgcttgacgc gcgacagctc gccgatctcg gccttgagcg aggtcagcgc
    21421 gctgatgatg gcctggcgcg cgccggcctt gccttcctcg acggagattt cgcccagctt
    21481 gcccttgtgg gtctgcgtgg ggccgttgaa ggtgccgtgg ccggccacga agaccagctt
    21541 gctggcggtg ctgacggcgc cgcagtagtt gcccatgggc gtgcgcttgg gcggcagttc
    21601 gatgccgagt tcggccaggc gttcttcggg ggtcttgcct ttgagcgtct tgaagatttc
    21661 gagagacatg ctgggctcct ggaaaggatc ggggtgtggg gtcaggcttt gtcgcgctgg
    21721 aggcgcaact gggtggccgc gcccgcggtc agcagggcgt ggatggagaa ttcgtcgcgc
    21781 aggcggtcgg tgtcgagcgt ggcgtgcacg gccatgaggg cgtcccgttc gccggcggcc
    21841 agcgcgtagc cgtcacagaa ggcggccgga tcggccagga aggcgcgggc gccggccggg
    21901 tcggtgcgca gcctgaacac cgccgcgacc agggccgcgt gatgctgcgg cagggccggg
    21961 tagatgagcg gcgcggggct ggcgggctcg gtgaggtccc aggcgtgcac gctgtagatg
    22021 tggtgccacg agggttcgaa cagcgccatg aatggcttgc ggttgcccgc cacggccgcc
    22081 gccgccaggg tcatgcgcag ctcgacgttg ccggtcttgt cgagcgcggc gctgtcgtag
    22141 gtcagcaggt gggtcagatt gccggcgctc atctgctcga agatcacctt gtcggtctcg
    22201 aggtcggggt gcggatagtg cgcggtgccg gggtagtgcg acaggccgcc gctggcgatc
    22261 agcacggcac gcttgcccag gcgcgccacg gcgcgggcca gcgcctggcc gaaggcgtgg
    22321 cagcgcagcg gcgagggctg cggcggcaca taggcgttga cgaagatcgc cgtcagcggc
    22381 acgtcgcgcg agaagcccat gaactcgtag ggaatggtga aggccgtggg cagcttggca
    22441 tcgagcgtga aggccaggtc gaagccttcc tcgcccatgc attgcaccag cggataggcc
    22501 atgccggcgt ccagcgtcca gttgccgcgg tgcttgtgca tgccgtcggc ctggggcgcc
    22561 gcatgcacgc agaagggcgg cacggtatgc gtgacgaagt ggtcgccgtg gtcgttggac
    22621 agcatgatga tgcagtcggg ttcgagcgcg cgcaggcgtt cgccgagctg ccgcatggcc
    22681 gcgtgcaccc ggtcgacctg ggcgaggtct tcgctgtcgg ggcgcgacag gaactgcggc
    22741 gcgtgcggaa cgcaggccac gccgacgatg ccgggttggg aatgcttcat ggtgtgatct
    22801 cctcgcgggt atgggcaatg gggcggcgtg tgcgtgggcc cgcatgcgct cggttagcaa
    22861 cagcgtgttg ccgccgggct gaccgccagg gcaacggcaa ctgcgcagca ggccagcgcc
    22921 aggcgaccgc tgtgtcgggc acgccggaaa gaatgtggta agtggagcat taaagaatac
    22981 tattaataag taaagtatac tttaaagcgg gaataatatc tgtccgtggt aacatgtcaa
    23041 gaaaaaagac acggcatgcg cgtaatcacg aatcaacgga cccgcgaacg gacttgaaat
    23101 gaccgcccta gacgaacatg tcgacccagg tacaccagaa aacggggaag gatcagggcg
    23161 gctgcgccgg ccggcggggg tgtatgaagc gctgttccag gagatagtca gcggcaagct
    23221 gcgcgccggc gagcgcctgc tgatcgacga tctggccgcg cgtttcgggg tgagcaagat
    23281 tccggtgcgc gaggccctga aggcgctgga aggcaagggc tgggtcgagt cggccccgcg
    23341 gcgcggcacc tatgtgcgcc ggctgtcccg ggaagaactg cacgaactct ttgagttgcg
    23401 tctgatcgtc gagcccgaga tcacggccat ggcggcccac cggcgccgcg cctcgcactt
    23461 gcaggagctg gctgccttca tcgaagaggg cgaggccgcc gtgcaggtca gcgatttcgt
    23521 gaccagctcg cggatcaatt cccagtttca tgcggtcatt gcccaggcgg caggcaatag
    23581 cctggcctat gcggtcatcc aggatctgga gctgcggcta tgccgctatt tcttcgtggc
    23641 cgagtgggag gagcgcaggg agtccatcct gcagcatcgc tccctgtacg aggctatccg
    23701 cgaccaggac gtcgaggcgg cgcgcaaggt gacgatcgcg cacctgcgcc ataccgaagc
    23761 catggcgtcg cgttcgctgc tggtccggcg catggccggg ctggaggaag acaagtagcc
    23821 cgcgcaggcg gcttggcggg cctgccgggc aggcgcccca atcggcgcgc aaaacaaaat
    23881 ggcgcgcaaa acaaaaagga tgccgggtag gcatcctttt tgttttgccg attcagttag
    23941 ccaaccccgt tgcggcattg ggcctgcgca gagacgatgc ggcaggcctg cgggcgcgta
    24001 tggctgggga ggtgtggcta gtcagtcgat caggccagcg ccttgacggc ggcagccagg
    24061 cggctcttgt ggcgagcggc cttgttcttg tggatgatgt tcttgtcggc cacgcggtcg
    24121 atcacgccgg tggctttttg cagcgtttcg ctggcaacgg ccttgtcgcc ggcgtcgatc
    24181 gcctggcgaa cgcgcttgat cgcggttcgc agcatcgaac gcagcgagga attatgtttg
    24241 ttgcgctcaa ccgattgacg ggcgcgctta cgagcttggg cggtattggc catggtttat
    24301 ctgaaataaa gattcggcaa agtccagcat tttagcgggc tttcccttgc ggcgcaaggg
    24361 cttgtctggg gggcgggcaa gaaggcaggc gcagttcagc ggtctgcgcc tgtcattcat
    24421 accgatgcga catcgattgc ggtgaaccag ggagtgatcc ggtgcacctg gatgggcatg
    24481 tcggcctcca gggcacgcag ggcggcgttg gtgtcgtcca gcggatagcg gccgcgcgcc
    24541 agcaggccgc ccgccgccat cgagatccgc agcacgcctt cgcggtaggg gcgcagggcg
    24601 gtgatgacct cggccagcgg ccggtcgcgg gcttcgatcc agccgttttc ccaggcggca
    24661 tcggccagca ggtcggctcg gggttcgccg atgcgggtat tgtcgaaacg ggtgccggtg
    24721 ccggcgggga tgatttgccg cgtgccggac gaggtttcga tttcgacctc gttgtcgggg
    24781 gcgatgacta gggtgcggcg ggcttgctgg cggaccacga cgcgcgcgcc gagcgcctgg
    24841 accaggcctt cgcccgtgcg gatcaggagg ggacgccccg ggtcgggttg cgcggtgagc
    24901 gagagggcgc cggcgaccag ctgcagggtg cgttcgctgg cggtatagga cagattgatc
    24961 cgggtgcgcg cgtccagcag catctggctg ccgtcggata agcggtacag gcggcgttcg
    25021 cccgtctggg tggccatgtc gttgcccagg ccggccaggg ggtagtaggc attgacggcc
    25081 gcggcgccgg cgacggtcgc gccgcccagc gccagcaggc cgcccaggaa gtggcggcgg
    25141 ttcagcccct ggccgactcc gcgcgtcgcc ggcttgtcgt gggtctcggg gttggcatag
    25201 gggtaggcat cgctgagccg cccggtttcg ccgctgctgc gcagggtgtc gttgagctgc
    25261 tgccaggcgc tttcgttgcg tgggtccgct gctcgccagg cgacgaagct ggcgtgatcg
    25321 gccggcgtag cgcggccgga gcgcagcagc accagccagt tcacgacctg atcggaaaga
    25381 ggttgccaag agagattcat ggttacacct cgcaagcttg cctgacagag ggcaacctgc
    25441 tggttgctac atgtataagc aggtgagatc gctaatgatt aacaagtact aatagtttac
    25501 gaaaatttac ctcgaatcaa tttgccactg caacccaaca actgcaaatt ttataatcaa
    25561 atttggtagt taacgttaat atttttgtga acttaaggga agataaacgg attttttgta
    25621 tatatatgta ttgattgata aatatattta cacaaaatgc cgggaatcca gcattttcgt
    25681 tgttacagaa ttcgtccaat agacgaatcg agatacttat tgatagagag tggaaagcaa
    25741 aaggcccgcc gtgcatggca ggccccttta cgggcggcgg gggattcagc ggccgccgac
    25801 gccgcggtgg tcgaagtccg ccggccggaa accggtggcg aacagcaggc cgaaatagac
    25861 ggccgcggag gcggccagga cgccagccag ccacagggcg cgctggcccg catgggtctg
    25921 cagcgcgatc cagtccagcc gatgctcggc ataccagagc aagacgccca ttccggccag
    25981 cgcgggcagc atgcgcagga agaagcggcc ccagcccggc gcgggccggt agacgccgcg
    26041 gcggcgcaag ccgatgagca gggtgccggc attgacgcag gcgcccaggc cgatggacag
    26101 ggccaggccg gcgtgggcga aaatcggcac gaatagcagg ttgaagccct gcgtcagcac
    26161 cagtacggcg atggcgatct tgaccggggt acggatgtcc tggcgggcgt agaagccggg
    26221 cgccaggatc ttcacggcca agaggccgat caggccgacg gagtaggcga tgacggccag
    26281 gtgggtctgt cgcacgtctt cggcgtggaa ggcgccgtag ttgaacagcg tggccaccag
    26341 gccgtcggac agcagggcca tgccgatggc ggccggcagg cccagcagca gcgtcaggcg
    26401 caaacctccg tcgatcaggt tgctataggt gtcgtggtcg ttcttggcgt gggccgccga
    26461 gaggctgggt agcagcacgg tgcccagcgc cacgcccagg agcgccgtgg ggaattccat
    26521 gagccggtcg gcgaacgaga gccaggtcac gctgccgggc ctgagccagg tggcgacgtt
    26581 ggtgttgatg agcagtgaga tctgcgccac ggacacgccc agggtggcgg gcgccatctg
    26641 cctgatgatg cgctgcacgg tggcgtcgtg ccaggcctgg gtcaggcggg tcgacagcag
    26701 gcgcggcgcc aggccgatat gcgccagttc ggcccattgc acggccagtt gggcgacgcc
    26761 gcccgccatg acgccgatgg ccaggccgta gatcggcagg cccatgcgtg gcgacagcca
    26821 caggcaggcg gcgatcatgc acaggttgag cagcaccggc gtgagggcgg ggagggcgaa
    26881 gcggcgccag gtgttgagca cgccggtggc gaaggccgcc agcgacatgc acaggatgta
    26941 cgggaacatg atgcgcgtca tggccacggc ggcgccgaat tcatggccgc gcccggtatc
    27001 gcgcaggccg ctggccatca gcgtcaccac ccagggcgcg gcgatgatgc ccaccacggt
    27061 gagcaatgcc agcacgatgg tcagcagcag ggccacgcgg tcgagcaggg ggcggatgcc
    27121 gtcgtcgcca tgctgggcgc gggccgtgcc gagaatgggc acgaaggcct gcgagaaggc
    27181 gccttcggca aacaggcggc gcagcaggtt ggggatgcga aaggccaccc agaaggcatc
    27241 ggtgagcgga ctggcgccga aggtgcgggc gatcaggatg tcgcgggcca acccggtgat
    27301 gcgggacagc agcgtcaggc cgctgatcgt ggcggctgat ttgaacaagg ccatttattt
    27361 gggttcttga gtcgtctgtt tcagggctgg cgccgccaag gcccgccagg gcttcgctac
    27421 ggcgggctcg ggcccaagac gagggggccc tcggccctgc gggctacccc gccgcagagg
    27481 cgccccgacg ggccctggcg gcgccagccc cgaaacagac tgcgtcagag ctgagcgtgg
    27541 ggcggccggt tcttttgctt atttcggcgg catgcggatc gcgccgtcga ggcggatcgt
    27601 ttcgccgttg agcatctcgt tggtgacgat gctgtagacc agcttggcgt agtcttcggg
    27661 gcggcccagg cgcgagggga aggggatggc ggcggccagc gaatcctgca cttcctgcgg
    27721 catgccgaag atcatgggcg tgccgaagat gccgggggcg atggtcatgc agcggatgcc
    27781 cgtgcgggcg aggtcgcgcg cgatgggcag tgtcatgccg accacgccgg ccttggaggc
    27841 cgcgtaggcg gcctggccga tctggccgtc gaaagccgcc accgaggcgg tgttgatcag
    27901 cacgccgcgc tcgccggtgg gctcgggcgt gttggcgctc atggcctcgg cggccaggcg
    27961 catcatgttg aagctgccga tcaggttgat cgagacgacc ttctggaaga gcgccagcgg
    28021 atgcgcgccg tccttgccca cgatcttgga ggcgggcgcg atgccggcgc agttcaccag
    28081 gccgaacacc gggccgagcg acttggcctg ggcgaccacg gcctgcgcgt cggcttcctg
    28141 ggtaacgtcg cagtgcacgt agtactggcc cagctccttg gccagcgcct ggccgggttc
    28201 gtcctgcagg tcggcgatga cgaccttggc gccgttggcc gtcagcatgc gcacggtgcc
    28261 ggcgcccagg cccgaggcgc cgccggtgac gatgaatacc ttgtttgcga tttccatatt
    28321 cgggtgtctc tctcgtgggg gttctcgatg cggcggcgag gagcgcgccg tgtcagcctt
    28381 gcagggcgtc gagcaggcgc tgggtgattt cctcgacgct gcccacgccg gatatcttgc
    28441 ggtacttggg ggcggcggcg ggctcgctga ccgaccagac cgagtagtag tcgaccagcg
    28501 ggcgggtctg ctgctggtag acgctgaggc ggttgcgcac ggtttcctcg cggtcgtcgt
    28561 cgcgctgcac cagcggttcg ccggtgacgt cgtccttgcc ttcgaccttg ggcgggttga
    28621 acaccgcgtg gtagctgcgg ccgctggccg gatgcacgcg gcggccgctc atgcgggtga
    28681 tgatgtcgct gtcgggcact tcgatctcga ccacgtagtc gagcttcacg ccggcgctct
    28741 ttaacgcatc ggcctgcggg atggtgcggg gaaagccgtc gaacaggtag ccggccgcgc
    28801 agtcgggctg cttgaggcgg tcctgcacca ggccgatgat gattccgtcg gataccaggc
    28861 cgcctgcgtc catgaccttc ttggcttcga tgcccagcgg cgtgccggcc ttgacggcgg
    28921 cgcgcagcat gtcgcccgtg gagatctgcg ggatgccgta gcgctgggtc agaaaagcgg
    28981 cttgagtgcc tttgccggcg ccgggcggtc cgagcaggat aagacgcatt gtgtactcct
    29041 ggatagggtt tttgtctgga tgtctggcgg gctgttgccg cccggcgggc agggcggcaa
    29101 cgctgaaact ttaactttta taacctattt gcgtaccagg cccggacgcg ttcgaggtcg
    29161 gcgggggtgt ccacgccggc cgcgggggcc tgggccgcgc ggtgcaccgc gatggcatgg
    29221 ccgtattcca tggcgcgcag ctgctcgagc gattcgaact tttccagcag gccttgctgc
    29281 agcagcggaa agcggcgcag gaatgatacg cggtaggcgt acaggccgat gtggtgccat
    29341 gccggcaggc cgggcgccag aacgcgatcg ccgccagcca gcgcgtcccg cgcccaaggg
    29401 atgggcgcgc gcgagaagta cagggcgcgg ccgtcctggg cgcacaccac cttcacggca
    29461 ttgggattga agagtgcctc ggcatcggcc agcgggcagg cgcaggtggc gatgtcggcc
    29521 cagggcgagg cggccaggca gtcggccacc gcgtcgatga gggcggggtc gatgagcggt
    29581 tcgtcgccct gcacgttgac caccacggca tcgtcgggca ggcccagcag ttgcacggcc
    29641 tcggccaggc ggtcggtgcc ggtggcgtgg tcggcgcggg tcatcagcgc cgcatagccg
    29701 tgcgcttcgg cggcggcctt gacgcgcgtg tcgtcggtgg ccacgcagac gcggcgggcg
    29761 ccggactggc tggcgcgttc ggccacgcgc acgaccatgg gcttgccggc gatgtcggcc
    29821 agcggcttgt ccggcaagcg ggtggaggcg gctcgggcgg ggatgagtac ggtgtagtcc
    29881 acggcgcggc aggactcagg ccgcgggcgt ggccgcagtc gtcacgtcga ggtcgcgcgc
    29941 ctcggactcc agcatgacgg ggatgccgtc gcgcacgggg aaggccagcc ggtcggcccg
    30001 gcagacgagt tcgctctgcg cgcggtcgaa ttgcaaacga cccttgcaaa ggggacacac
    30061 gaggatgtcg agcaggcggg attccatggc cggaattcta gcgcgcaatg gccccggcca
    30121 gccaggcggc caggtcc
//
LOCUS       DASWPO010000211         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_47581, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000211 DASWPO010000000
VERSION     DASWPO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47581"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGC69_11170"
     CDS             <1..191
                     /locus_tag="VGC69_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HEY0295882.1"
                     /translation="DFARTRLPDRLVTAGITDLMPRIFATGLAPVEHACGLTLLGLDL
                     SASMRAPLARHFLQGLRT"
     gene            446..1453
                     /gene="dusB"
                     /locus_tag="VGC69_11175"
     CDS             446..1453
                     /gene="dusB"
                     /locus_tag="VGC69_11175"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY0295883.1"
                     /translation="MQIGPWTLPNNVLVAPMAGVTDRPFRQLCKRLGAGYAVSEMAAS
                     NPKLWDSVKTSRRLNHDGEIAPVSVQIAGADPAMMAEAAAFNAQRGARIVDINMGCPA
                     KKVCNLASGSALLRHEDLIQRILDAVVAACAPLGVPVTLKTRTGWDREHKNALRIARM
                     AQDAGIAALTLHGRTRADLYAGQAEYDTIREVKAALDIPVVANGDIDSPEKARAVLDY
                     TGADAVMIGRAAQGRPWIFREIDHYLRTGNKLPAPSYAEMRDLLLEHLDDHYRFYGEH
                     TGVRTARKHIGWYIEGLPDAQTFRIRMNLIDNTADQARAVARWFGRLAQAGGEPQRLA
                     A"
     gene            1501..1740
                     /locus_tag="VGC69_11180"
     CDS             1501..1740
                     /locus_tag="VGC69_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0295884.1"
                     /translation="MSKQSVLEECVRASLARYFEDLDGAEPHDMWDMVMRCVERPVLE
                     VAMERAGGNQSRASEMLGITRNTLRKKLLAHNIQP"
     gene            1754..>1946
                     /gene="purH"
                     /locus_tag="VGC69_11185"
     CDS             1754..>1946
                     /gene="purH"
                     /locus_tag="VGC69_11185"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019849.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0295885.1"
                     /translation="MKIETALLSVSDKTGIVEFAQALHSRGVRLLSTGGTAKQLAAAG
                     LPVTEVAQHTGSPEILDGRV"
BASE COUNT          367 a          748 c          543 g          288 t
ORIGIN      
        1 ccgatttcgc ccgcacccgc ctgcccgacc gcctcgtcac cgccggcatc accgacctca
       61 tgccccgcat cttcgccacc ggcctggctc cggtcgagca cgcttgcgga ttgacgctcc
      121 tggggctgga cctctccgcc tcgatgcgcg caccgttggc aaggcatttt ctgcagggac
      181 tgcggactta aatcaattga gttcttaatc cgttcgtcgg gaggcgcagt ggaggcgccg
      241 gaccctcggc ggggctcggg cccaagacga cgggccctcg gccccctgcg ggggctgccc
      301 ccgcctcaac ggtccggcgc ccccactgcg cttcccgacg aactcacaac atcttgcatt
      361 gaaacgcagc gcctatgcga ctcaagaacc caaaaaccgc aacccagacc accccacctc
      421 cagtctggtt aaaatctgag caatcatgca aatcggcccc tggaccctcc ccaacaatgt
      481 gctcgtcgcc cccatggccg gcgtcaccga ccgacccttc cggcagcttt gcaagcgcct
      541 gggagccggc tatgccgtgt ccgagatggc cgccagcaat cccaagctct gggacagcgt
      601 caagacctcc cgccgcctga accacgacgg cgaaatcgcc ccggtgtccg tccagatcgc
      661 cggcgccgac cccgccatga tggccgaggc cgccgccttc aacgcccagc gcggcgcccg
      721 catcgtcgac atcaacatgg gttgccccgc caagaaagtg tgcaacctcg ccagcggctc
      781 cgccctgctg cgccacgaag accttatcca acgcatcctc gacgccgtcg tcgcggcctg
      841 cgccccgctc ggcgtacccg tgaccctgaa aacccgcacc ggctgggacc gcgaacacaa
      901 gaacgccctg cgcatcgccc gcatggccca ggatgccggc atcgccgccc tgaccctgca
      961 cggccgcacc cgcgccgacc tctacgccgg ccaagccgaa tacgacacca tccgcgaagt
     1021 caaggccgcc ctcgacatcc ccgtcgtggc caacggcgac atcgactcgc ccgagaaagc
     1081 cagggccgtc ctggattaca ctggcgccga cgccgtcatg atcggacgcg ctgcccaggg
     1141 ccggccctgg atattccgcg aaatcgatca ttacctgcgc accggcaaca agctgcccgc
     1201 gccctcatat gccgagatgc gcgacctgct gctggaacac ctggacgacc actaccgctt
     1261 ctacggcgag cacaccggcg tacgcacggc gcgcaagcac atcggctggt acatcgaagg
     1321 actgcccgat gctcaaactt tccgtatccg catgaacctg atcgacaaca ccgccgatca
     1381 ggcgcgggcc gtggcgcggt ggttcggccg gttggcgcag gccggcggcg aaccgcaacg
     1441 cctggcggcc tgacggcccg cctgtcttta ccctacacat agaacgatct gtacctaaaa
     1501 atgagcaagc aaagtgttct ggaagaatgc gtgcgcgcca gcctggcgcg ctatttcgaa
     1561 gacctggacg gcgcagagcc ccacgacatg tgggacatgg tgatgcgctg cgtcgaacgg
     1621 cccgtcctcg aggtggccat ggaacgcgcc ggcggcaacc agtcgcgcgc ttccgaaatg
     1681 ctcggcatca cccgcaatac cctgcgcaag aaactgctgg cgcacaacat ccagccctga
     1741 gtcctcttaa tccatgaaaa tcgaaaccgc cctcctctcg gtctccgaca agaccggcat
     1801 cgtcgaattc gcccaggccc tgcacagccg cggcgtgcgc ctgctttcca ccggcggcac
     1861 ggccaagcag ttggccgcgg ccggcctgcc cgtgaccgaa gtggcccagc acaccggctc
     1921 gcccgagatc ctcgacggcc gcgtca
//
LOCUS       DASWPO010000212        24008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3083546, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000212 DASWPO010000000
VERSION     DASWPO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 24008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24008
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3083546"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGC69_11190"
     CDS             <1..648
                     /locus_tag="VGC69_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEY0295886.1"
                     /translation="LVEHRMAFLTQYQNAAYAKRYRALVDKVAQAEQVTTGTNRLALA
                     VARYYFKLMAYKDEYEVARLYSDGAFIKQIESMFEGDWKLNLHLAPPTFAKHDKQGHL
                     VKRRYGPWMLKAFRVLAGLRGLRGTALDVFGYTEERRAERGLISQYAEDITAILAKLE
                     RGNLDRAVALASLPEQIRGYGHIKEASMAKAAARRAELLKELSAQIVRLGGSRAA"
     gene            complement(1233..2501)
                     /locus_tag="VGC69_11195"
     CDS             complement(1233..2501)
                     /locus_tag="VGC69_11195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0295887.1"
                     /translation="MDDIAPCVAVIGLDENKNLVVTACNSQFFRLTGGLRADTQTFPI
                     PLDTLMADPAPRGFSEKLLACFSSSRPQEPEQACDLREGGRWWRLALKPIRHASGDAG
                     ARQVLVTGFDMGTKTNPAQEPEDANASRYRALVDLAYDAIVTMDRQHNITFFNRAAEN
                     LFGYSSAEVLGRPITTLMPDKFRANHPQKVQQFADSYQPSLRRATPPRMDESNSVYGQ
                     HRDGSVIPVEIAVSKLDLNSGTEFMAIVRDITDRARLMEMLKKQAATDALTGLPNRRE
                     FLKFIEKELGTHDDLSVFILDIDFFKEINDQHGHDAGDEVLRVLAKIGTSMMDGKSLF
                     ARWGGEEFVAALPGADANLAYRIADELRQRYERQDFEQAWRVRPIPFTVSIGVVTREA
                     DEIDVDALMKRADRALYRAKKSGRNRVEAG"
     gene            complement(2642..3151)
                     /locus_tag="VGC69_11200"
     CDS             complement(2642..3151)
                     /locus_tag="VGC69_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295888.1"
                     /translation="MRPKRLARIGAQVLAGASLVLACQAAYALSSDARECRASGGKLL
                     TGQVVSAPKFKHGMYKKGVELSHTHLRLQGDDGNTYDVAIDNVFASGYRPNSRRVPAP
                     LDTIEVGDKIEACGIPFKGGIHWVHNNCGDTSTRSDPNGWLKVVQTSGGTGPNLEDNQ
                     AYCRLWPRR"
     gene            complement(3241..3891)
                     /gene="cheD"
                     /locus_tag="VGC69_11205"
     CDS             complement(3241..3891)
                     /gene="cheD"
                     /locus_tag="VGC69_11205"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HEY0295889.1"
                     /translation="MPSASKARATRYYYDSAFDCPAVKILPNEYYVTTGEELMLSTVL
                     GSCVSACIHDPVTKVGGMNHFMLPEGDSTSPASATMRYGSFAMEVLINELIKAGAERS
                     RLEAKVFGGGAVLAAMQQMNIGERNGAFVLNYLKTEGIPVRAQDLGDVHARRINYFPR
                     SGKVMVRRMAPHHHKGEILIAKRDEITAKVVKAEAQTPAPVQRFTTQGGMKIERFK"
     gene            complement(4029..4937)
                     /locus_tag="VGC69_11210"
     CDS             complement(4029..4937)
                     /locus_tag="VGC69_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY0295890.1"
                     /translation="MRALLQQHRYALVTTLRRLAAQPFSSLANLLVMALALALPLLGA
                     AVLVSVEPVARQLSVTPELTVFMQPGAPGDAAQSVAARIKSEAAGQVEAVRVVPRAQA
                     LDSLRNNPAWREALAALPDNPLPDAVVVTLTRGDDLAARAGSLAGQWGKWPQVDHVQL
                     DSAWVQRLEAILQFGRIGLLFLAVCVAVVVLATVFNTVRLQAMTQREEIAVARLVGAT
                     ESFVRRPFLYLGALTGVAAALLAILAAGLALQPLNGAVLGLARSYGAEFALHLPSLPV
                     LAIEVLAAGILGALSARWSVTRSTRF"
     gene            complement(4934..5635)
                     /locus_tag="VGC69_11215"
     CDS             complement(4934..5635)
                     /locus_tag="VGC69_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0295891.1"
                     /translation="MIEFQHVFKSYGRGRNILADINFRITAGEFVFVSGPSGAGKSTL
                     LKLVGGLEAPSRGSIQVNGQRLDRLTPRARPYLRRAVGVILQDTHLLYDRSALANVML
                     PLMVAGLPAGTASGRARAALDKVGLAGKEDLSPIELSGGEQQRLAIARAIVNRPAILI
                     ADEPTANLDHGNAQRILNVFRDFNRVGVTTLISSHDTELMSGYANRVLRIPEPGKFLD
                     SHPTNSQSSDAGAPA"
     gene            5930..6946
                     /locus_tag="VGC69_11220"
     CDS             5930..6946
                     /locus_tag="VGC69_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0295892.1"
                     /translation="MKALKLVAIGATLFAASTAAHAGATFDNVQKKGFVQCGVSTGLQ
                     GFSLADSKGEWQGIDVDVCRAIAATMFGDASKVKYTPLSSQQRFTALQSGEVDLLSRN
                     TTQTITRDTTLGLIGTGVDYYDSQGVMVKKDLGVKSAKELAGATVCVQPGTTTELNLA
                     DWFRAQKLEFKPVVIEKMEEAVNAFVAGRCDAYTTDKSGLAATRATLANPDQYIILPE
                     DFSKEPLGPMVRQGDEQWFNVVRWSLNAMLEAEEYGITSKNADEELKSSNPNVQRILG
                     VTPGAGKGMGVDDKWAYNIVKQVGNYGEVFERNLGMDSPLKLVRGLNASYKNGGIQYG
                     WPIR"
     gene            6996..8183
                     /locus_tag="VGC69_11225"
     CDS             6996..8183
                     /locus_tag="VGC69_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0295893.1"
                     /translation="MTSSSNSTASAPARRLSWGDPGVRSVVYQIVAVGIVGLAIWFLI
                     SNLLHNLNSRHITTGFAFLSRESGFAISETLIDYSPASTYGRAICVGILNTLIISVLG
                     IVFATLLGTLVGIGRLSRNWLIGRLATVYVEAIRNVPLLLQLFFWYSIISEVMPGPRL
                     AHQPLPGVFLSNRGLKIPALAGESVDWMLLGLLLAVVGIFVLRYWGRRRQQATGQPFP
                     LGRSAIALLIVLPFLGWLLSGSHLHLDMPALRGFNFAGGWTLSPELAALLFGLVIYTA
                     SFIAEVVRSGIQAVDNGQWEAAGSLGLRRGLVLRLVVLPQALRVIIPPMTSQYLNLTK
                     NSSLAVAIGYPDIVSILNTTMNQTGQAIEGVLIIMAAYLTVSLAISFFMNWYNKRMAL
                     VER"
     gene            8239..9336
                     /locus_tag="VGC69_11230"
     CDS             8239..9336
                     /locus_tag="VGC69_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0295894.1"
                     /translation="MSTATPTPEASLPPPNRIGAGHWIRTRLFSSPLNALLTILAAWF
                     LLMAVPALLEWVLIKAHFTGTSEVCRADRSGACWSFIVDKHRLILFGTYPYEQQWRPL
                     AATLVMVAMVVAGGMRRFWNLKIVVIWTAGLVLSGVLMWGGVLGLGYVEDSLWGGLPV
                     TLLLSVFGIILAFPLGVMLALGRRSDMPAIKTLCVVYIELIRGVPLISMLFMASVMIP
                     LFLPEGFNIDKLLRAQIAIIMFTAAYIAETVRGGLQAIPKGQYEGADSIGLSYWQKMR
                     KIILPQALRLVIPPIVSTFISLFKDTSLVVIIGTFDLMLTAKTAMSDVAWRGFSAEGY
                     VFIGLIYFAFCFSMSKYSQALEKRLRTGYSR"
     gene            9356..10096
                     /locus_tag="VGC69_11235"
     CDS             9356..10096
                     /locus_tag="VGC69_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0295895.1"
                     /translation="MANAIIRLNQVNKWYGQFHVLRDINLEVKQGERIVICGPSGSGK
                     STMIRCINRLEEHQQGQIIVDGTELTNDLRQIEAIRREVGMVFQHFNLFPHLTVLENL
                     TLGPTWVLKKPLAEARATAMKYLERVRIPEQADKYPGQLSGGQQQRVAIARSLCMIPK
                     VMLFDEPTSALDPEMVKEVLDVMVRLAEESGMTMLCVTHEMNFARKVADRVIFMDRGE
                     IIEENSPDLFFDSPQNERTKLFLSQILH"
     gene            10318..11340
                     /locus_tag="VGC69_11240"
     CDS             10318..11340
                     /locus_tag="VGC69_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295896.1"
                     /translation="MTHFSLFPSPVRRALVGGALALALGLSAAPLLAQAASDYPDKPL
                     RFIVPYPPGGPLDTMARLLAQKASPALGQPIIVENRAGAGGNIGADLIAKAAPDGYTL
                     GMGAVATNAINPWLFAKLPYDPIKDFAPITIVASVPNVLDVNVAFAQKNNIKTLNDLI
                     AYAKAHPGRLNFGSGGNGSAGHLSGALLNARAGISTQHIPYQGASPAQLALLSGQSDF
                     MFDNLAASAPLIKDGKVLALAVTTTKRSSLLPAVPTVQEAGVKDFDLGTWFGIMTTGG
                     TPPEIVAKLNKAYSDALRQPDVRKVLLEMGSEIGPMSAAEFTEFVKNEKDKYQEIVKI
                     SGANVN"
     gene            11340..11750
                     /locus_tag="VGC69_11245"
     CDS             11340..11750
                     /locus_tag="VGC69_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0295897.1"
                     /translation="MLYIWLRLLHVAVLTIWLLGMLAASVACFPSGEAQAPSRFVLGM
                     ARWNSRVTTPAMLLTWLCGLSLASMAGWFGQNWLTAKIVIALVLSGVHGLQTAGLRRL
                     CAGERAPGVLIVSGMVVVLGAMVALSLAFVKPDF"
     gene            complement(11755..12459)
                     /locus_tag="VGC69_11250"
     CDS             complement(11755..12459)
                     /locus_tag="VGC69_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295898.1"
                     /translation="MLRVEKLEAGYGPSKVLFGVDLEIAPRQVVSLIGRNGMGKTTTV
                     RTLMGMLPAQGGRVLLDGKPIASLAPHRIARLGLGLVPEGRRVFASLSVEENLIATSR
                     GATPGNRASWHLGRVYELFPRLKERRLQSARTLSGGEQQMLAVGRALLTNPRLLILDE
                     ATEGLAPLIRLEIWSCLRRLKEDGQTILVIDKNLKEMAALVDRHHVLEKGRVVWEGTP
                     DAFGKNPGLANRYLGV"
     gene            complement(12459..13298)
                     /locus_tag="VGC69_11255"
     CDS             complement(12459..13298)
                     /locus_tag="VGC69_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295899.1"
                     /translation="MIEQQARLDIRDLRKSFGAVQATQGVNLSVRPGELHALIGPNGA
                     GKTTLLSQIAGELLPDSGSIRLDGRDITRMPAQQRPAAGLARSFQISQVYPEFTVEDN
                     AAMAVQAHTPGGFNLWRDARRDLRLREPARRALARVGLAHRAGERVANLAHGEKRQLE
                     LAMALCMDASLLLLDEPMAGLGAEESLRMAELLRELKGQYAILLVEHDMDIVFSLADR
                     ITVLVYGKTLFTGAPDEVRVHPEVRAAYLGEEDASVAGPPEDGCAPPGGGKLAGRSVG
                     GKQ"
     gene            complement(13291..14259)
                     /locus_tag="VGC69_11260"
     CDS             complement(13291..14259)
                     /locus_tag="VGC69_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0295900.1"
                     /translation="MSRKLLINAAGLLLFALIPAIAAATGTPFLVNLCTQFAIYALAA
                     VSLDLILGYGAMVSFGHAAFFAIGGYTVGIIGFHMGQGDTLLGWAGSGSALVIWPLAV
                     IIAAIAGLAVGFLSLRTSGVQFIMITLAFGQMLYFILVGLMVYGGDDGLSIDARNTLP
                     GVDLGSPTAFYYTCLVLMALWIGLCRRIVRSPFGMALRAIKQNARRSVALGYAPLRYR
                     LTAFVLSAAGAGLAGALWANYALFVSPDMAAWQKSGELMAMVILGGLGSIFGPVAGVA
                     LYLGLEQTATAFTEHWMLILGPVLVLVVLFGKRGIYGWLTGGRHHD"
     gene            complement(14267..15181)
                     /locus_tag="VGC69_11265"
     CDS             complement(14267..15181)
                     /locus_tag="VGC69_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0295901.1"
                     /translation="MLMLLEQALNGLQYSALLFLLSAGLTLVFGIMNVINLTHGSFYM
                     VGAFCAAAAMAKTGSFLAAILAALAGAGLYGLVIELLVIRHLYRRDHLDQVLATLGLA
                     LFTNELVTVLFGRSPPLMNIPPVLAGSVQVLPGLAYPVMRLALIGAGALVAVGLWWLI
                     SRTRVGMLVRAGADDGEMVDALGIDIQRLFTLIFALGALLCGFAGVMAAPLLAVEIGM
                     GERILITTFVVIVVGGVGSVRGALVGSLLIGMTDAMGRAFIPRLTSMLLPPQLSDPLS
                     SSLVSASIYILMAIILLFKPRGLVAARH"
     gene            complement(15198..16376)
                     /locus_tag="VGC69_11270"
     CDS             complement(15198..16376)
                     /locus_tag="VGC69_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295902.1"
                     /translation="MKQHSAHRIAAALLLAAAGSAPALAQQAVKIGFITTLSTPAGYL
                     GEDERDGFMLAVKEGGGKLGGVPVDVRVEDDGLKPASAKQIADKMVQDGIRLFTGINF
                     SNVMAAVGPTVLNAGGFYVSLNAGPSNYAGRACNKNYFDAAFQNDSFSDTAGMVANDL
                     GAKRMVVMAPNYQAGRDAVAGFKSTYKGQIVDEIYTKLDQTDFSVELARIRSLEPDAI
                     FQFHPGGVGINLAKQFANSGLADKIKMITPIFSMDDRMLAASGAAAKGYYLSALWSAD
                     LPNAQNKHFIEAFTKTYNRVPTTSAAQAYDTANLIASALKAVDGDVTGRADAFRDALR
                     KADFPSVRGNFKFAANQHPIQDWYLLHVEDDGTGKLVFKNLKVIARDHVDPHVADCKL
                     "
     gene            complement(16421..17629)
                     /locus_tag="VGC69_11275"
     CDS             complement(16421..17629)
                     /locus_tag="VGC69_11275"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HEY0295903.1"
                     /translation="MNAASTPQSAIVVGGGIGGLAAALALTRQGIRVQLLEQAAEIGE
                     IGAGIQLGPNAFAALDALGVGQIARQRAVFTDHIIMMDAVDNTEVVRIETGREFQERF
                     NGPYAVIHRADIHGAILEGVKHDPLISFHTSTHIKRFHQADDYAEVVDEQGKAYRADI
                     VVGCDGVKSVVREHLIGDPPRVTGHVVYRAVVERENMPEDLRINAPVLWAGPRCHLVH
                     YPLRGGAQYNLVVTFHSREPEEWGVRDGSKEEVLSYFQGIHARPRQLLDTPSSWRRWA
                     TADREPVEQWSYGRATILGDAAHPMSQYMAQGACMALEDAVTLGLAVAGHKDDLRAAF
                     KLYESLRIPRSARVVWSTREMGRIYHAKGVERAVRNSLWQGRSQQSFYDALQWLYGWN
                     AENCMRGIRL"
     gene            complement(17646..18995)
                     /locus_tag="VGC69_11280"
     CDS             complement(17646..18995)
                     /locus_tag="VGC69_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0295904.1"
                     /translation="MHITEPARQVPLHGEFEVVVLGGGPAGILAAVSAARNGARVLLI
                     ERYGFLGGMGTAAGVSNFCGLHANIHGDIRQVVHGMTDELLDRMRALDGLNDPHLILG
                     KIHAQAYDLSAFKCAADGMVQASGAQLLFHALATGVARDAEGRIDTLFLETKSGRCAV
                     RGRVFIDCSGDADLAHWAGLPYEKGDADGHMMYPTLMFKVGNVDSARAQEAWKTIPQL
                     MDAAAASGEFTFPRRGAIVRPQKHDYEWRVNVTQLANRDGSAADGTDADSLSAAELEG
                     RRQIVDYLAFLRAKVPGFEQAYILDIAPQLGIRETRRIVGEYVLTREDVLGCADFPDS
                     IGVNGWPLEIHTAGDVQWVWPPIPESRGYNQLPFRMMVPRQGPGIPDNVLVAGRCASM
                     THEGQSAARVSGSCFVMGEAAGTAAAMALRDGIPPARLAPAALQRQLEHQGAFLGIR"
     gene            complement(19027..20022)
                     /locus_tag="VGC69_11285"
     CDS             complement(19027..20022)
                     /locus_tag="VGC69_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295905.1"
                     /translation="MNKIQTPGLLRSILAAGLTALALAAGAAHADTWPSRPLRLIVPF
                     GAGSSPDQAARVIGEKLGGILDQSVVVDNRPGANGNLGTFDIASAKPDGYTFGVSITG
                     PLVNNTLLYDNLPYKPATDLAPLTLAVHQPNVLVVPANSGITDINGLLDALKKNPGKY
                     NFPSPGAGTVSQLAVELLLQQIGASATHIPYPSSPAALTSLLAGDTQFAALPPVAVMG
                     MVKSGRLRALAVTSAKRSSLLPDIPTLAEVGVPGIEGSAWIGFVTSAKVPADVQKKLS
                     DALIQAIHDPDVSQKLRSLFMDPVGSTPAEFRSYMDDELKRWGPLIDKLGLKGKV"
     gene            complement(19988..20392)
                     /locus_tag="VGC69_11290"
     CDS             complement(19988..20392)
                     /locus_tag="VGC69_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY0295906.1"
                     /translation="MQEPDAQGQPLRAYIDPAYRPLCATLAEVRHNIDRLDDEIVRLV
                     AQRAMYVKDAARFKRDAFQVGAPARQAEVFDKVRRLAQRHDQGFANLEQVVEAAYRAM
                     VAAFIANEQAYFDKMKNIGDNHEQDPNPGAAA"
     gene            complement(20400..21044)
                     /gene="maiA"
                     /locus_tag="VGC69_11295"
     CDS             complement(20400..21044)
                     /gene="maiA"
                     /locus_tag="VGC69_11295"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY0295907.1"
                     /translation="MQLYSFFNSSTSYRVRIALALKGLSYDYAGVDIRIKKHREADYV
                     ALNPNANVPLLRDDGFDLGQSLAIIDYLDATHPEPRLIPADPKLRARVLELSNVIACD
                     IHPVNNLRILKYLVGELGVSAEKKDEWYAHWIREGLTTVEELLARHGSSAGPYCFGDQ
                     PTMADCCLVPQLYNAIRMKCDMSAYPRAVKVYEHCIGQPAFMQAAPSVQPDFKQ"
     gene            complement(21045..21746)
                     /locus_tag="VGC69_11300"
     CDS             complement(21045..21746)
                     /locus_tag="VGC69_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0295908.1"
                     /translation="MSYVFAPQAPIGVPVAGSNDLFPVRRVYCVGRNYAAHAREMGFD
                     PDREPPFFFCKPADAVVPVAAGSTLELAYPGETSNYHYEIELVAAIGKAGSNIGAAQA
                     LEHVWGYAVGLDMTRRDLQMKMREAGRPWELGKAFDLSAPIAPLYPATQAGDIHQARI
                     WLKVNGETKQSSEIPKLIWSVAETVSYLSKFFRLEPGDLIYTGTPEGVGPVVAGDLME
                     GGVDGLGTLNVRVTK"
     gene            22129..23061
                     /locus_tag="VGC69_11305"
     CDS             22129..23061
                     /locus_tag="VGC69_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295909.1"
                     /translation="MDWTHRLRLRNVKMLLSLAQTRNISHSAAMLNTTQPGLSKWLKD
                     LEEDIGLPLFERHARGLVPTHYGEVLIEHARRLDTQLDRASRDMAALREGSAGRVVIG
                     ASGAAASDTAPLAMLRLLDRMPQASVSLVEGTTDRLLGQLAQGDLDLVMGRAGPERTD
                     PAIEMESLYRDPIRLVARPHHPLLSRERLSWEDLRAYRWIIWPQGTPVRTALNEALAE
                     AGEALPRDAVETNSVTSNLTLLNNSDMVGVASHRAAVRLAQMNTLRILPIRMSGFGAV
                     ALYWRHESFRPQAVQVAMDCIREVVAEYAAVEGV"
     gene            complement(23058..23582)
                     /locus_tag="VGC69_11310"
     CDS             complement(23058..23582)
                     /locus_tag="VGC69_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY0295910.1"
                     /translation="MRRFVSAVPLTLACIVSPPATAGAAPVQAVQKVDLNRYVGTWYE
                     IANFPRFFLPHCVADTVSEYTANGDGTFAIKNRCRTRDGGTNEAYGTATPLQGSDNTR
                     MEVTFMRPFSADYVVVGLDPDYRWTVIGSVNRKSLWILSRTPQLPKPELDQALSVASA
                     QGYPMQKLHYTPQD"
     gene            complement(23650..>24008)
                     /locus_tag="VGC69_11315"
     CDS             complement(23650..>24008)
                     /locus_tag="VGC69_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY0295911.1"
                     /translation="AELLACMQADSGIALSELRADGGAARNDLLMQMQADLLGVPVVR
                     PRVLESTALGAAGLAGLAVGFWSSQDELAGHWQAERRFEPAWSADRREARMARWRQAV
                     EHAKGWARPLDPASAL"
BASE COUNT         3801 a         7720 c         8354 g         4133 t
ORIGIN      
        1 ctggtcgaac accgcatggc cttcctcacg caataccaga acgcggccta tgcgaaacgc
       61 tatcgcgcgc tggtcgacaa ggtggcgcag gcggagcagg tgacgaccgg caccaaccgg
      121 ctggcgctgg cggtggcgcg ctattacttc aagctcatgg cctacaagga tgagtacgag
      181 gtggcgcgcc tgtatagcga cggcgctttc atcaagcaga tcgagtcgat gttcgagggc
      241 gattggaagc tcaacctgca cctggctccg ccgacgttcg ccaagcacga caagcaaggc
      301 catctggtca agcggcgcta tggtccctgg atgctgaagg ccttccgcgt gctggcgggt
      361 ttgcgcggtc tgagggggac ggcgctggac gtgttcggct atacggagga gcggcgcgcg
      421 gagcgcgggc tgatcagcca gtatgccgag gacatcacgg ccattctggc caagctcgag
      481 cgcggcaacc tggatcgcgc ggtggccttg gcgagtttgc ccgagcagat ccgcgggtat
      541 gggcacatca aggaagcctc gatggccaag gcggcggcgc ggcgcgcgga attgctcaag
      601 gaactgagcg cgcagatcgt gcggctgggc gggtcgcggg cggcctgatg gcgccctgac
      661 gacgtcaagg cgccaagctc gctggccggg cttggcgctc gacccctgtc gatgaacgcc
      721 cgcaaattcg tcggcctatg acaggcgctg accttcgcgg ccgtctgctt gcgcttcgcg
      781 ggagacagtc agggcatctg gcggcggcgc gtcattgttc ccgacttgga tcaatacctc
      841 cgggtactgc tcgaggcaat acgcgccgag gctaccgaaa taggtcacgc agcgatcagc
      901 gcatccttat cgaaaggcgg gacgtccacc gagcctgtgg ccaagggctc caatatttcc
      961 gcgagtttct gcagaaccgt gcgaagcgca gtccgctctc cctcgtcgag agctgagaag
     1021 aaatgatcgt cgttgttgtc cgcaatttct gtcgaacagg caaccggcgt cgggcatcgc
     1081 gcgtgagcga caggaacagg acgcggaaag cgcagaactt gtcaggcccc gagtccgttc
     1141 gatcttgttc agcgcgaccc actcggcggt tgaaatatcg tgatgactga gcgagcgtgc
     1201 gaacgcatta ttgattccat gttcgcaacc cgctatccgg cctcgacgcg attgcggccg
     1261 gatttcttcg cccggtagag cgcccggtcg gcacgcttca tcagcgcgtc gacatcgatc
     1321 tcgtcggctt cgcgcgtgac cacgccgatg ctgaccgtga atggaatcgg cctgacacgc
     1381 catgcctgct cgaagtcctg tcgttcgtag cgctgccgca gctcgtccgc gatccggtac
     1441 gcaagatttg cgtccgcccc tggcagggct gcgacaaact cttcgccgcc ccagcgggcg
     1501 aacaggctct tgccgtccat catcgacgtc ccgatcttcg cgagcacacg caagacttca
     1561 tcgccagcgt cgtgcccatg ctggtcgttg atctccttga agaagtcgat gtcgagtatg
     1621 aacacggaca aatcgtcatg ggtaccaagc tctttttcta tgaacttgag aaactcgcgg
     1681 cgattcggca ggcctgtcag cgcgtcggtc gcggcctgct ttttcagcat ttccattaac
     1741 cgggcgcgat cggtgatgtc gcggacgatc gccatgaatt cggtcccgct gttcaagtcg
     1801 agtttggaga ccgcgatttc gaccggtatg accgacccgt cgcgatgttg cccgtacacg
     1861 ctgttgcttt cgtccatgcg cggcggggtc gcgcgccgca ggctcggctg atacgagtcc
     1921 gcgaactgct gcactttttg cggatggttc gcgcgaaact tgtccggcat aagggttgtg
     1981 atcggccgcc ccagcacctc ggccgacgaa tagccaaaga gattctccgc cgcgcgattg
     2041 aaaaacgtga tgttgtgctg gcgatccatg gtcacgatcg cgtcgtaagc caagtcgacc
     2101 agggcgcgat aacgcgaggc attggcgtct tccggctctt gtgccggatt ggtcttcgtg
     2161 cccatgtcga aaccggtcac gagaacctgg cgcgcgccgg cgtcgccgct tgcgtgcctg
     2221 atcggtttga gcgcaaggcg ccaccaccgg ccgccttcgc ggaggtcgca agcctgctcc
     2281 ggctcttgcg gtcggctgga cgaaaagcac gcgagcagtt tctcgctaaa cccgcgcggc
     2341 gccggatccg ccatcagcgt gtcgagtgga atggggaaag tctgcgtatc cgcgcgcaat
     2401 ccacccgtca atcggaagaa ttggctattg caggcggtga cgacgagatt cttgttctcg
     2461 tcgagcccga tcacggcgac gcatggggcg atatcgtcca cgacctagtt cagttccttg
     2521 gacaaatcag acaaaacctt acctggcaaa agtgctgcgc agcaccagtg tccgcttcga
     2581 tggaattgtc gcacaaggtt ggcgcgctcc ctgtttcgca acaattgtgc agccccctgg
     2641 tctatcggcg cggccagagg cggcagtagg cctgattgtc ttcgaggttc gggccggtgc
     2701 cgccgctggt ttgaaccacc ttcagccagc cattcgggtc cgacctcgtg gacgtatccc
     2761 cgcaattatt gtggacccaa tggatgccgc ccttgaacgg aatgccgcag gcctcgatct
     2821 tgtcgcccac ctcgatggtg tccagcggcg cgggcactct tctcgagttc ggccggtatc
     2881 ccgaggcaaa gacattgtcg atggcgacgt cgtaggtgtt gccgtcgtcg ccctgcagcc
     2941 gcaggtgcgt gtgcgacagc tcgacgcctt ttttgtacat gccgtgcttg aacttcggcg
     3001 cggacaccac ctggccggtg agcaattttc cgccggatgc cctgcattcc cgggcgtcgc
     3061 tggacaaggc gtaggcggcc tggcaggcca ggaccagcga ggcgccggcc aggacctgcg
     3121 ccccgatccg ggcaaggcgc tttgggcgca tgggtaaagg cgacatgggc ttgctccgtc
     3181 cgttcatctc ggccgaatta tgcgcccagt ttctccgggc ccggcatccg aaagtcctgc
     3241 ctacttgaag cgctcgatct tcatcccgcc ctgggtcgta aaccgctgca ccggcgccgg
     3301 cgtctgggcc tcggccttga ccaccttggc cgtgatctcg tcgcgcttgg cgatgaggat
     3361 ctcgcccttg tggtggtggg gcgccatccg ccgcaccatc accttgccgc tgcggggaaa
     3421 ataattgatg cggcgggcat gcacgtcccc caggtcctgc gcgcggaccg ggatgccttc
     3481 ggtcttcagg taattgagca cgaacgcgcc gttgcgctcg ccgatgttca tctgctgcat
     3541 cgccgcgagc accgccccgc cgccgaacac cttggcttcc aggcggctgc gctccgcgcc
     3601 ggccttgatc agttcgttga tgagcacttc catggcgaac gagccgtagc gcatcgtggc
     3661 cgacgccggc gacgtgctgt cgccctcggg cagcatgaaa tggttcatgc cgcccacctt
     3721 ggtcaccggg tcgtggatgc aggccgatac gcatgagccc agcaccgtcg agagcatcag
     3781 ctcctcgccg gtggtgacgt aatactcgtt cggcaggatc tttactgcgg ggcagtcgaa
     3841 ggcgctgtcg tagtagtagc gggtagcccg ggctttggag gcagatggca tggcgggtaa
     3901 gagtggtacg gctccgccgt tacatcatga cgggtaagtc cgaaattgtt acatattgcg
     3961 atgcagcggt cccgattcgg gccaatgtag ccggtcggcc ccctggttca atcctcagat
     4021 tagggccgtt aaaagcgcgt actacgggtc actgaccagc gtgccgagag agcgcccagg
     4081 atgccggccg ccagcacctc gatcgccagc acgggcaggc tgggcaggtg cagggcgaat
     4141 tcggcgccgt agctgcgggc caggcccagg acggcgccat tgagcggctg cagggccagg
     4201 ccggcggcca ggatggccag cagggcggcg gccacgcccg tcagggcgcc caggtagagg
     4261 aagggccggc gcacgaagga ctcggtggcg cccaccagcc gggccaccgc gatctcctcg
     4321 cgctgggtca tggcctgcag gcgcacggtg ttgaagaccg tggccagcac caccacggcc
     4381 acgcagacgg ccaggaacag caggccgatg cggccgaact gcaggatggc ttccaggcgc
     4441 tgcacccagg cgctgtcgag ctgcacgtgg tccacctggg gccacttgcc ccattgtccg
     4501 gccagcgagc cggcgcgggc ggccaggtcg tcgccgcggg tcagggtgac caccacggcg
     4561 tcgggcaggg ggttgtcggg cagggcggcc agggcttcgc gccaggccgg gttgttgcgc
     4621 agggagtcca gggcctgggc gcgcggcacc acgcgcacgg cctcgacctg gccggcggcc
     4681 tcgctcttga tgcgggccgc cacggattgg gcggcgtcgc cgggggcgcc cggctgcatg
     4741 aagacggtga gctcgggggt gaccgacaac tggcgggcca ccggttccac cgacaccagc
     4801 acggcggcgc ccagcagcgg caaggccagg gccagggcca tgaccagcag gttggccagc
     4861 gaggaaaacg gttgggcggc gagccggcgc agcgtggtga cgagcgcgta gcggtgctgt
     4921 tgcagcaggg ccctcatgcc ggggctcccg cgtcgctgga ttgcgaattc gtggggtgcg
     4981 agtccaggaa cttgccgggc tcggggatgc gcagcacgcg gttggcatag ccggacatca
     5041 gttcggtgtc gtgcgaggag atcagggtgg tcacgcccac ccggttgaag tcgcggaaga
     5101 cgttcaggat gcgctgggca ttgccgtggt cgagattggc cgtgggctcg tcggcgatca
     5161 ggatggcggg gcggttgacg atggcgcggg cgatggccag gcgctgctgc tcgccgccgg
     5221 agagctcgat ggggctgagg tcttccttgc cggccaggcc caccttgtcg agcgcggcgc
     5281 gcgcgcgccc gctggcggtg ccggccggca ggccggcgac catcagcggc agcatgacgt
     5341 tggccagcgc gctgcggtcg tagagcaggt gcgtgtcctg caggatcacg ccgacggcgc
     5401 ggcgcaggta gggccgcgcg cgcggcgtga gccggtccag ccgctggccg ttgacctgga
     5461 tgctgccccg gctgggcgct tcgaggccgc ccaccagctt gagcagggtg gacttgccgg
     5521 cgcccgacgg gcccgatacg aagacgaatt ctccggccgt gatgcggaag ttgatgtcgg
     5581 ctaggatatt gcggccgcgt ccgtacgatt tgaaaacgtg ctggaactcg atcatggcga
     5641 tgcgcaagtg aagccgcaat agtaaccgct catggggcgg gcctgcatgg cgtgcatgcc
     5701 tggcgcggcg ccgcgcggcg ggatcgggcc tgcgcagcta aataacacaa gcttgctact
     5761 aaataaccct aaatacttat aggtgtcccc taattggtgg caaattgcaa agggtttccc
     5821 cccttgtccc catatatcgg tccacgtacg atcccgccgt gcgcatcgaa taccgataac
     5881 cagaccggag ccggtgccgc gctcgttgtt tttattggga gaacacataa tgaaagctct
     5941 aaaactcgtg gcgatcggcg cgacgctgtt cgccgcctcc acggcggcgc acgcgggcgc
     6001 caccttcgac aacgtccaga agaaaggttt cgtccagtgc ggcgtgtcca ctggcctgca
     6061 gggcttttcc ctggctgaca gcaagggcga gtggcaaggc atagacgtcg acgtgtgccg
     6121 cgccatcgcc gccaccatgt tcggcgatgc cagcaaggtc aagtacaccc ccctgagctc
     6181 ccagcagcgc ttcacggccc tgcagtccgg cgaggtcgac ctgctcagcc gcaataccac
     6241 ccagaccatc acccgcgaca ccaccctggg cctgatcggc accggcgtcg actactacga
     6301 cagccagggc gtcatggtca agaaggacct gggcgtcaag agcgccaagg aactggccgg
     6361 cgccacggtc tgcgtgcagc ccggcaccac caccgaactc aacctggccg actggttccg
     6421 cgcccagaag ctcgaattca agccggtcgt catcgagaag atggaagagg ccgtcaatgc
     6481 cttcgtggcc ggccgctgcg acgcctacac gaccgacaag tcgggccttg ccgccacgcg
     6541 cgccacgctg gccaaccccg accagtacat catcctgccg gaagacttct ccaaggagcc
     6601 gctgggcccc atggtgcgcc agggcgacga gcagtggttc aacgtcgtgc gctggtcgct
     6661 caatgccatg ctcgaggccg aggaatacgg catcacctcg aagaacgccg acgaagagct
     6721 caagagctcc aaccccaacg tgcagcgcat cctgggcgtg accccgggcg ccggcaaggg
     6781 catgggcgtg gacgacaagt gggcctacaa catcgtcaag caggtgggca actacggcga
     6841 agtcttcgag cgcaacctgg gcatggacag cccgctcaag ctggtccgcg gcctgaacgc
     6901 ctcgtacaag aacggcggca tccagtacgg ctggccgatc cgctgagcgc tttctgcacg
     6961 cggcgtgccg gcggcacgcc gcgacccgcc cccctatgac ttcatcctcc aacagcactg
     7021 cgtccgcgcc cgcgcggcgg ctcagctggg gcgatcccgg cgtcaggtcg gtggtctacc
     7081 agatcgtggc ggtcgggatc gtcggcctgg cgatctggtt cctgatctcc aacctgctgc
     7141 acaacctcaa ttcacgccac atcacgaccg ggttcgcctt cctgagccgc gaatccggct
     7201 ttgccatcag cgagacgctg atcgattatt cgccggccag cacctacggc cgggccatct
     7261 gcgtaggcat cctcaacacc ttgatcatct cggtgctggg catcgttttc gccaccttgc
     7321 tgggcacgct cgtcggcatc ggccggctgt ccaggaactg gctgatcggc cggctggcca
     7381 cggtgtatgt cgaagccata cgcaacgtgc cgctgctgct gcagctgttc ttttggtatt
     7441 cgatcatcag cgaggtcatg ccggggccgc gcctggcgca ccagcctttg ccgggcgtgt
     7501 tcctgtccaa ccgcggcctg aagatccccg cgctggccgg cgagtcggtc gactggatgc
     7561 tgctgggcct gctgctggcc gtcgtcggca tcttcgtcct gcgctactgg ggcaggcggc
     7621 gccagcaggc cacagggcag ccctttcccc tggggcgcag cgccatcgcc ttgctcatcg
     7681 tcctgccttt cctgggctgg ctgctgagcg gcagccacct gcatctcgac atgcccgcgc
     7741 tgcgcggctt caacttcgcc ggcggctgga cgctgtcgcc cgagctggcg gccctgctgt
     7801 tcggcctggt catctatacg gcgtccttca tcgcggaggt ggtgcgctcg ggcatccagg
     7861 ccgtcgacaa cggccagtgg gaggcggccg gctcgctggg cctgcgccgc ggcctggtgc
     7921 tgcgcctggt ggtgctgccg caggcgctgc gcgtgatcat tccccccatg accagccagt
     7981 acctcaacct caccaagaac agctcgctgg ccgtggccat cggctatccc gacatcgtgt
     8041 ccatcctcaa caccacgatg aaccagacgg gccaggccat cgagggcgtg ctcatcatca
     8101 tggcggccta tctcaccgtg agcctggcga tctccttctt catgaactgg tacaacaagc
     8161 gcatggcgct ggtggagcgt tgatgaaacg gcccccttgc ccgctgcgtt ggcggccctc
     8221 cggcggggcg tgccaaccat gagcaccgcc acgccaacgc ccgaagccag cctgccgccg
     8281 ccgaaccgca tcggcgcggg ccactggata cgcacgcggc tgttctcttc gccgctcaac
     8341 gcgctgctga ccatcctggc ggcatggttc ctcctgatgg ccgtgccggc gctgctggag
     8401 tgggtcctga tcaaggccca tttcaccggc acgtccgagg tgtgccgcgc cgaccgctcc
     8461 ggcgcctgct ggagcttcat cgtcgacaag catcgcctga tcctgttcgg cacctatccc
     8521 tacgaacagc agtggcgccc gctcgcggcc accctcgtca tggtcgccat ggtggtggcg
     8581 ggcggcatgc gccgcttctg gaacctcaag atcgtcgtca tctggacggc cggcctggtg
     8641 ctgtcgggcg tgctgatgtg gggcggcgtg ctggggctgg gctatgtcga ggactccctg
     8701 tggggcggcc tgccggtgac gctgctgctg tcggtgttcg gcatcatcct ggcgtttccg
     8761 ctgggcgtga tgctggcgct ggggcggcgc tcggacatgc cggccatcaa gacgctgtgc
     8821 gtggtctaca tcgaactgat ccgcggcgtg ccgctcatca gcatgctgtt catggcctcg
     8881 gtgatgatcc cgctcttcct gcccgagggc ttcaatatcg acaagctgct gcgcgcgcag
     8941 atcgccatca tcatgttcac ggcggcctat atcgccgaga ccgtgcgcgg cggcctgcag
     9001 gccatcccca aggggcagta cgagggggcg gattccatcg gcctgtcgta ctggcagaag
     9061 atgcgcaaga tcatcctgcc gcaggcgctg cggctggtca tcccgcccat cgtcagcacc
     9121 ttcatctcgc tcttcaagga cacgtcgctg gtggtgatca tcggcacctt cgacctgatg
     9181 ctcacggcca agacggccat gtcggacgtg gcctggcgcg gcttcagcgc cgaaggctat
     9241 gtgttcatcg ggctgatcta cttcgcgttc tgcttttcca tgtccaagta cagccaggcc
     9301 ctggaaaagc ggctgcgcac cggctattca cgatagagtc accaggagtc ccggcatggc
     9361 caatgcgatc atccgcctga atcaggtcaa caagtggtat gggcagttcc acgtgctgcg
     9421 cgacatcaat ctcgaggtca agcagggcga gcgcatcgtg atctgcggcc cctcgggctc
     9481 gggcaagtcc acgatgatcc gctgcatcaa ccggctggaa gaacaccagc agggccagat
     9541 catcgtcgac ggcaccgagc tcaccaacga cctgcgccag atcgaggcca tccgccgcga
     9601 ggtgggcatg gtgttccagc acttcaacct gttcccccac ctgacggtgc tggagaacct
     9661 gaccctgggg cctacctggg tgctcaagaa gccgctggcc gaggccaggg caacggccat
     9721 gaagtacctg gagcgcgtgc gcattcccga gcaggccgac aaataccccg gccagctctc
     9781 gggcggccag cagcagcgcg tggcgatcgc gcgctcgctg tgcatgatcc ccaaggtcat
     9841 gctgttcgac gagccgacct cggcgctcga cccggaaatg gtcaaggaag tgctggacgt
     9901 catggtgcgg ctggcggagg aaagcggcat gaccatgctg tgcgtcacgc acgagatgaa
     9961 cttcgcccgc aaggtggccg accgcgtgat cttcatggac cgcggcgaga tcatcgagga
    10021 aaacagcccg gacctgttct tcgacagtcc gcagaatgag cggacgaagc tgttcttgag
    10081 tcagattctg cattgagttc ttgatccaca gcaccgccgc cggcctgggc ggcttcgctg
    10141 agccgggctc gggcgcgcca tcaggcgccc tcggcccctt cggggcttcc ccgtctcaga
    10201 ggcgccgccc aggccggcgg ccggcggcgg cgctgttcca gcgtctcgga tgtgagacga
    10261 ctgcggcgcg gctgctgggg tatgcttgtc ggcaatttgc taacaaggag acgcctcatg
    10321 acacactttt ccctgtttcc ctcgccggtg cgccgtgccc tggtgggcgg cgcgctggcc
    10381 ctggccctgg ggttgtccgc cgccccgttg cttgcgcagg ccgcctccga ctatcccgac
    10441 aagccgctgc gcttcatcgt gccttacccg cccggcggtc cgctggacac catggcgcgc
    10501 ctgctggcgc aaaaggccag ccccgcgctg ggccagccca tcatcgttga aaaccgcgcc
    10561 ggcgcgggcg gcaatatcgg cgccgacctg atcgccaagg ccgcgcccga cggctatacg
    10621 ctgggcatgg gcgcggtggc caccaacgcc atcaatccct ggctgttcgc caagctgccc
    10681 tacgacccca tcaaggactt cgcgcccatc accatcgtgg cctcggtgcc caatgtgctg
    10741 gacgtcaacg tcgctttcgc gcagaagaac aacatcaaga ccctgaacga cctgatcgcc
    10801 tatgccaagg cgcatccggg caggctcaac ttcggctcgg gcggcaacgg cagcgcgggc
    10861 cacctgtcgg gcgcgctgct caacgcgcgc gccggcatct cgacgcagca cattccctac
    10921 cagggggcgt cgccggccca gctggcattg ctgtcgggcc agtcggactt catgttcgac
    10981 aacctggccg cctcggcgcc gctgatcaag gacggcaagg tgctggcgct ggccgtgacc
    11041 acgaccaagc gttcgtcgct tctccctgct gtgccgacgg tgcaggaggc gggcgtcaag
    11101 gacttcgacc tgggcacctg gttcggcatc atgaccacgg gcggcacgcc gcccgagatc
    11161 gtggccaagc tgaacaaggc ctactcggac gccctgcgcc agcccgacgt gcgcaaggtt
    11221 ctgctcgaga tgggttccga aatcgggccc atgtccgcgg ccgagttcac cgagttcgtc
    11281 aagaacgaga aggacaagta ccaggaaatc gtcaagattt ccggtgcgaa cgtgaactga
    11341 tgctctacat ctggctcagg ctgctgcatg tggccgtgct cacgatctgg ctgctgggta
    11401 tgctggcggc gtcggtggcc tgctttcctt ccggcgaggc gcaggcgccc agccggttcg
    11461 tgctgggcat ggcgcgctgg aacagccggg tgacgacgcc ggccatgctg ctgacatggt
    11521 tgtgcgggct gtcgctggcg tccatggcgg gctggttcgg ccagaactgg ctgaccgcca
    11581 agatcgtgat cgcgctggtg ctgtcgggcg tgcacggcct gcagacggcg ggcctgcggc
    11641 gcctgtgcgc gggcgagcgc gcgccgggcg tgctcatcgt cagcggcatg gtggtcgttc
    11701 tgggcgccat ggtggcgctg tcgctggcgt tcgtgaagcc ggacttctga cgaatcagac
    11761 gcccaggtag cggttggcca gcccggggtt cttgccgaac gcgtcggggg tgccttccca
    11821 taccacgcgg cccttctcca gcacgtggtg ccggtccacc agcgcggcca tttccttgag
    11881 gttcttgtcg atcaccagga tcgtctggcc gtcttccttc aggcggcgca ggcagctcca
    11941 gatctccagc cggatgagcg gggccaggcc ttcggtggcc tcgtccagga tcaggaggcg
    12001 cgggttggtc agcagggcgc ggcccacggc cagcatctgc tgttcgccgc cggacagggt
    12061 gcgggccgat tgcaggcggc gctccttcag gcgcgggaag agttcgtaga cccgccccag
    12121 gtgccagctc gcgcggttgc ccggcgtggc gccgcgcgat gtcgcgatca ggttttcttc
    12181 cactgacagc gaggcgaaca cgcgccggcc ctcgggcacc aggcccaggc ccagccgcgc
    12241 gatgcggtgc ggcgccaggc tggcgatggg cttgccgtcg agcagcacgc gcccgccctg
    12301 ggcgggcagc atgcccatca gcgtcctgac cgtggtggtc ttgcccatgc cgttgcggcc
    12361 gatcagcgag accacttggc gcggggcgat ctccaggtcg acgccgaaca gcaccttgct
    12421 ggggccgtag cccgcttcga gtttttcgac gcgcagcatt attgcttgcc ccccacgctg
    12481 cgccccgcga gcttgccgcc ccccggggga gcgcatccgt cttcgggcgg cccggcgacg
    12541 gatgcgtctt cctcgcccag gtaggcggcg cgcacttccg ggtggacgcg gacttcgtcc
    12601 ggcgcgccgg tgaagagggt cttgccgtag accagcacgg tgatgcggtc ggccagcgag
    12661 aacacgatgt ccatgtcgtg ctcgaccagc aggatggcgt attggccctt gagttcgcgc
    12721 agcagttcgg ccatgcgcag cgattcctcg gcgcccaggc cggccatggg ttcgtccagc
    12781 agcagcagcg aggcgtccat gcacagggcc atggccagtt cgagctggcg cttctcgcca
    12841 tgggccaggt tggccacgcg ctcgccggcg cggtgcgcca ggccgacgcg cgccagggcc
    12901 cggcgggccg gctcgcgcag gcgcaggtcg cgccgcgcgt cgcgccacag gttgaagccg
    12961 cccggcgtgt gggcctgcac cgccatggcg gcgttgtcct cgaccgtgaa ttccggatag
    13021 acctggctga tctggaacga gcgggcgagc ccggcggccg ggcgctgctg cgccggcatg
    13081 cgggtgatgt cgcggccgtc gagccggatg ctgccgctgt cgggcaagag ttcgcccgcg
    13141 atctgcgaca gcagggtggt cttgccggcg ccgttggggc cgatcagcgc gtgcagctcg
    13201 cccgggcgca ccgacaggtt gacgccctgc gtggcctgga ccgcgccgaa ggacttgcgc
    13261 aggtcgcgga tgtcgagcct ggcctgttgt tcaatcatga tgcctgcctc cggtgagcca
    13321 gccgtagatg ccgcgcttgc cgaacagcac caccagcacc agcacggggc cgaggatcag
    13381 catccagtgt tcggtgaagg ccgtggcggt ctgctccagg cccaggtaga gggccacgcc
    13441 ggccacgggg ccgaaaatcg agcccaggcc gcccaggatg accatggcca tgagttcgcc
    13501 cgacttctgc caggcggcca tatcggggct gacgaagagc gcgtagttgg cccacagggc
    13561 gcctgccagg ccggcgccgg ccgccgacag gacgaaggcc gtgaggcggt agcgcagggg
    13621 cgcgtagccc agggccacgc tgcggcgggc gttctgcttg atggcgcgca gggccatgcc
    13681 gaagggcgag cgcacgatgc gccggcacag gcctatccac agcgccatca agaccaggca
    13741 ggtgtagtag aaggcggtgg gcgagcccag gtcgacgccg ggcagggtgt tgcgcgcatc
    13801 gatcgacagg ccgtcgtcgc cgccgtagac catcaggccc accaggatga agtacagcat
    13861 ctggccgaag gccagcgtga tcatgatgaa ctgcaccccc gaggtgcgca gcgacaggaa
    13921 gccgaccgcc aggccggcga tggcggcgat gatcacggcc agcggccaga tgaccagcgc
    13981 cgagccgctg ccggcccagc ccaggagggt gtcgccctgg cccatgtgaa agccgatgat
    14041 gccgacggtg tagccgccga tggcgaagaa ggccgcgtgg ccgaagctga ccatggcgcc
    14101 gtagcccagg atcaggtcga ggctgacggc ggccagcgca tagatggcga actgcgtgca
    14161 caggttgacc aggaagggcg tgccggtcgc cgcggcaatg gcggggatca atgcgaacag
    14221 cagcagcccg gccgcattga tgagcaattt acgggacatg gacacctcag tggcgcgcgg
    14281 cgacgaggcc gcgcggcttg aacagcagga tgatggccat caggatgtag atgctggccg
    14341 acaccaggct ggagctgagc gggtcgctca actgcggcgg cagcagcatg gacgtcagcc
    14401 tggggatgaa ggcgcggccc atcgcgtcgg tcatgccgat gagcagcgat cccaccaggg
    14461 ccccgcgcac cgatcccacg ccgcccacca cgatcaccac gaaggtggtg atgaggatgc
    14521 gctcgcccat gccgatctcc acggccagca gcggcgccgc catgacgccg gcaaagccgc
    14581 acagcagcgc gcccagggcg aagatcaggg tgaagaggcg ctggatgtcg atgcccagcg
    14641 cgtcgaccat ctcgccgtcg tcggcgccgg cgcgcaccag catgcccacg cgggtgcgcg
    14701 agatcagcca ccacaggccc acggccacca gcgcgccggc gccgatcagc gccaggcgca
    14761 tgaccggata ggccaggccg ggcaggacct gcaccgagcc ggccaggacg ggagggatgt
    14821 tcatgagcgg cgggctgcgg ccgaacagca ccgtgaccag ctcgttggtg aagagcgcca
    14881 ggcccagcgt ggccagcacc tggtcgagat ggtcgcgccg gtacaggtgc cggatcacca
    14941 gcagctcgat gaccaggccg tacaggccgg cgcccgccag cgcggccagg atggcggcga
    15001 ggaaggagcc ggtcttggcc atggcggcgg ccgcgcagaa ggcgccgacc atgtagaagg
    15061 agccatgcgt caggttgatg acgttcatga tcccgaatac cagggtcagg ccggccgaca
    15121 gcaggaacag cagggcgctg tattgcaggc cgttcagggc ctgctccaac aacatcagca
    15181 tggaatggct ccggtgctta cagcttgcag tcggcgacgt gcgggtcgac gtggtcgcgg
    15241 gcgatgacct tgaggttctt gaagaccagc ttgccggtgc cgtcgtcttc cacgtgcagc
    15301 aggtaccagt cctggatggg gtgctggttg gcggcgaact tgaagttgcc gcgcacgctg
    15361 gggaagtcgg ccttgcgcag cgcgtcgcgg aaggcgtcgg cgcggccggt gacgtcgccg
    15421 tccacggcct tcagcgccga ggcgatcagg ttggccgtgt cgtaggcctg cgccgcggac
    15481 gtggtcggca cgcggttgta ggtcttggtg aaggcttcga tgaaatgctt gttttgcgcg
    15541 ttgggcaagt cggcgctcca gagcgcgctc aggtagtagc ccttggcggc cgcgccgctg
    15601 gcggcgagca tgcggtcatc catcgagaag atcggggtga tcatcttgat cttgtcggcc
    15661 aggcccgagt tggcgaactg cttggccagg ttgatgccga cgccgcccgg gtggaactgg
    15721 aagatcgcgt cgggttcgag cgagcggatg cgcgccagct ccaccgagaa gtcggtctgg
    15781 tcgagcttgg tgtagatctc gtcgacgatc tggcccttgt aggtgctctt gaagccggcc
    15841 acggcatcgc gtccggcctg gtagttgggc gccatcacga ccatgcgctt ggcgcccagg
    15901 tcgttggcga ccatgccggc ggtgtcgctg aacgagtcgt tctggaacgc cgcgtcgaag
    15961 tagttcttgt tgcaggcgcg gccggcatag ttcgaggggc cggcgttcag gctcacgtag
    16021 aagccgccgg cgttgagcac ggtcggtccg acggcggcca tcacgttcga gaagttgatg
    16081 ccggtgaaca ggcggatgcc gtcctgcacc atcttgtcgg cgatctgctt ggcggaggcg
    16141 ggcttgaggc cgtcgtcctc gacccgcacg tccacgggca cgccgcccag cttgccgccg
    16201 ccttccttga cggccagcat gaagccgtcg cgctcgtcct cgccgagata gccggccggc
    16261 gtggacagcg tggtgatgaa gccgatcttc acggcctgct gggccagggc cggcgcgctg
    16321 ccggcggcgg ccagcagcag ggctgccgcg atgcgatggg cgctgtgttg tttcatggtg
    16381 tctcctcgtt gggatttatt ggatttatgg cgggcgcggc ctagagccgg atgccgcgca
    16441 tgcagttctc ggcgttccag ccgtacagcc actgcagggc gtcgtagaag gactgctgcg
    16501 agcggccttg ccacagcgag ttgcggaccg cgcgctcgac gcccttggcg tggtagatgc
    16561 ggcccatttc ccgggtcgac cagaccacgc gggcgctgcg cgggatgcgc agcgattcat
    16621 acagcttgaa cgcggcgcgc aggtcgtcct tgtgcccggc cacggccagg ccgagcgtca
    16681 cggcgtcttc cagggccatg caggcgccct gggccatgta ttgcgacatg gggtgggcgg
    16741 cgtctcccag gatggtggcg cggccatagc tccattgttc gacgggctcg cggtcggccg
    16801 tggcccagcg gcgccacgaa gacggcgtat cgagcagctg gcgcgggcgg gcgtggatgc
    16861 cctggaagta cgacagcact tcttccttgc tgccgtcgcg cacgccccat tcttccggtt
    16921 cgcggctgtg gaaggtgacg accaggttgt attgggcgcc gccgcgcagc ggatagtgca
    16981 ccaggtggca gcgcgggccg gcccacagca ccggcgcgtt gatgcgcagg tcctcgggca
    17041 tgttctcgcg ctcgaccacg gcgcggtaga ccacgtggcc ggtcacgcgc ggcgggtcgc
    17101 cgatgaggtg ctcgcgcacc accgacttca cgccgtcgca gcccaccacg atgtcggcgc
    17161 ggtaggcctt gccctgctcg tcgaccacct cggcgtagtc gtcggcctgg tggaagcgct
    17221 tgatgtgggt cgaggtgtga aaggagatca gcgggtcgtg cttgacgcct tccaggatgg
    17281 cgccgtggat gtcggcgcgg tggatcacgg cgtaggggcc gttgaagcgt tcctggaatt
    17341 cgcggccggt ctcgatgcgc acgacctcgg tgttgtcgac cgcgtccatc atgatgatgt
    17401 ggtcggtgaa cacggcgcgc tggcgggcga tttggcccac gcccagcgcg tcgagcgcgg
    17461 cgaaggcatt ggggccgagc tggatgccgg cgccgatttc gccgatctcg gcggcctgct
    17521 ccagcagttg cacccggatg ccctggcggg tcagcgccag cgcggcggcc aggccgccga
    17581 tgccgccgcc gaccacgatg gcggattgcg gggtcgatgc tgcgttcata gtcgtttcct
    17641 gtgattcaac ggatgcccag gaatgcgccc tggtgctcga gttgccgctg cagcgccgcc
    17701 ggcgccaggc gggcgggcgg gatgccgtcg cgcagggcca tcgccgccgc ggtgccggcc
    17761 gcttcgccca tgacgaagca gctgccgctg acgcgcgcgg cggattggcc ttcgtgcgtc
    17821 atggacgcgc agcggcccgc caccagcacg ttgtcgggga tgcccggccc ttgcctgggc
    17881 accatcatgc gaaacggcag ctggttgtag ccgcgcgatt cggggatggg cggccacacc
    17941 cactggacgt cgccggccgt gtggatttcc agcggccagc cattgacgcc gatgctgtcg
    18001 gggaagtccg cgcagcccag cacgtcttcg cgggtgagca cgtattcgcc gacgatgcgg
    18061 cgggtttcgc ggatgcccag ctgcggcgcg atgtccagga tgtaggcctg ttcgaagccc
    18121 gggaccttgg cgcgcaggaa ggccaggtag tcgacgatct ggcggcgccc ttcgagctcg
    18181 gcggccgaca gcgagtcggc gtcggtgccg tcggccgccg agccgtcgcg gttggccagc
    18241 tgcgtcacgt tgacgcgcca ttcgtagtcg tgcttttgcg ggcgcacgat ggcgccgcgc
    18301 cgcgggaagg tgaattcgcc gctggctgcg gcggcgtcca tcaattgcgg gatggtcttc
    18361 caggcttcct gcgcgcgcgc gctgtcgacg ttgccgacct tgaacatcag cgtggggtac
    18421 atcatgtgcc cgtcggcgtc gcccttttcg tagggcaggc cggcccagtg cgcgaggtcg
    18481 gcatcgcccg agcagtcgat gaagacccgg ccgcgcaccg cgcagcggcc ggacttggtc
    18541 tccaggaaca gcgtgtcgat gcggccttcg gcgtcgcgcg ccacgccggt ggccagcgcg
    18601 tggaacagca gctgcgcgcc gctggcctgc accatgccgt cggcggcgca cttgaaggcc
    18661 gagaggtcgt aggcctgcgc gtggatcttg cccaggatca ggtgcgggtc gttcaggccg
    18721 tccagggcgc gcatgcggtc gagcagctcg tcggtcatgc cgtgcacgac ctggcggatg
    18781 tcgccgtgga tgttggcgtg caggccgcag aaattggata cgccggccgc cgtgcccatg
    18841 ccgcccagga agccgtagcg ctcgatcagc agcacgcgcg cgccgttgcg cgccgcgctg
    18901 accgcggcga ggatgccggc ggggccgccg ccgagcacca cgacctcgaa ttcgccatga
    18961 aggggaacct gccgggcggg ttcggtgatg tgcatcgtgt tctcctgtgt gccgcgcggc
    19021 aggccgtcag accttgccct tgaggcccag cttgtcgatg agcgggcccc agcgcttgag
    19081 ttcgtcgtcc atatagctgc ggaattcggc cggcgtgctg ccgaccgggt ccatgaagag
    19141 cgagcgcagc ttctggctca cgtcggggtc gtggatggcc tggatcagcg cgtcggacag
    19201 tttcttctgg acgtcggccg gtaccttggc cgaggtcacg aagccgatcc aggccgagcc
    19261 ctcgatgccg ggcacgccga cctcggccag cgtgggaatg tcgggcagca gcgacgaacg
    19321 cttggccgag gtgacggcca gcgcccgcag gcggccgctc ttgaccatgc ccatgaccgc
    19381 gaccggcggc agcgcggcga actgggtgtc gccggccagc agcgaggtca gcgcggccgg
    19441 ggacgagggg tacggtatgt gggtggcgct cgcgccgatc tgctgcagga gcagttcgac
    19501 ggccagttgc gagacggtgc cggcgcccgg cgaggggaag ttgtatttgc cggggttttt
    19561 cttgagggcg tcgagcaggc cgttgatgtc ggtaatgccc gagttggccg gcaccaccag
    19621 cacgttgggc tggtgcacgg ccagcgtcag cggcgccagg tcggtggcgg gcttgtaggg
    19681 caggttgtcg tacagcagcg tgttgttcac cagggggccg gtgatggaca cgccgaaggt
    19741 gtagccgtcg ggcttggcgc tggcgatgtc gaaggtgccg aggttgccgt tggcgcccgg
    19801 gcggttgtcg accacgacgc tctggtccag gatgccgccg agcttttcgc cgatgacgcg
    19861 cgccgcctgg tcggggctgg agcccgcgcc aaagggcacg atgagccgca gcggccggct
    19921 gggccaggtg tccgcgtggg cggcgccggc ggccagcgcc agcgcggtca ggcctgctgc
    19981 caggatgcta cgcagcagcc ccggggtttg gatcttgttc atggttgtct ccgatatttt
    20041 tcattttgtc gaaatacgcc tgctcgttgg cgatgaaggc cgccaccatg gcgcggtagg
    20101 cggcttccac gacctgttcc aggttggcga agccttggtc gtggcgctgc gccagcctgc
    20161 gcaccttgtc gaagacctcg gcctgccgcg ccggggcgcc gacctggaag gcgtcgcgct
    20221 tgaagcgggc cgcgtctttc acatacatgg cgcgctgcgc gaccaaccgg acgatctcgt
    20281 cgtcgagacg gtcgatgttg tgccgcacct cggccagcgt ggcgcacaag ggccggtagg
    20341 ccgggtcgat gtaggcgcgc agcggctggc cttgggcgtc gggctcttgc atggccggct
    20401 tattgcttga agtcgggttg gacgctgggc gcggcctgca tgaatgccgg ctgcccgatg
    20461 cagtgttcgt agaccttgac ggcgcgcggg taggccgaca tgtcgcactt catgcggatg
    20521 gcgttgtaca gctgcggcac caggcagcaa tccgccatgg tgggctggtc gccgaagcag
    20581 taggggccgg cgctgctgcc gtggcgcgcc agcagttcct cgacggtggt caggccttcg
    20641 cggatccagt gcgcgtacca ctcgtctttt ttctcggcgc ttacgcccag ctcgcccacc
    20701 aggtacttca ggatgcgcag gttgttcacc ggatggatgt cgcaggcgat gacgttgctc
    20761 agttccagca cccgggcgcg cagcttgggg tcggccggga tcaggcgcgg ctcgggatgc
    20821 gtggcgtcga gataatcgat gatggccagc gactggccca ggtcgaagcc gtcgtcgcgc
    20881 agcagcggca cgttggcgtt ggggttgagc gcgacgtagt cggcctcgcg gtgcttcttg
    20941 atgcggatgt ccacgcccgc gtagtcgtag gacaggccct tgagcgccag cgcgatgcgc
    21001 acgcggtacg aggtggagct gttgaagaag ctgtagagct gcatttattt ggtcactcga
    21061 acgttgaggg tgcccaggcc gtccacgccg ccttccatca ggtcgccggc cacgacgggg
    21121 cccacgcctt ccggggtgcc cgtgtagatc aggtcgcccg gctccagccg gaagaacttg
    21181 gacaggtagg acaccgtctc ggccaccgac cagatcagct tggggatctc gctggactgc
    21241 ttggtctcgc cattgacctt caaccagatc ctggcttggt ggatgtcgcc ggcttgggta
    21301 gcaggataca acggcgcgat cggggcgctc aggtcgaaag ccttgcccag ctcccagggg
    21361 cggccggcct cgcgcatctt catctgcagg tcgcggcgcg tcatgtccag gcccacggca
    21421 tagccccaga cgtgctcgag cgcctgtgcc gcgccgatgt tcgagccggc cttgccaatg
    21481 gcggcgacca gttcgatctc gtagtggtag ttggacgttt cgccggggta ggccagctcg
    21541 agcgtgctgc cggcggccac gggcacgacg gcgtcggccg gcttgcagaa gaagaagggc
    21601 ggctcgcggt cggggtcgaa gcccatttca cgggcgtggg cggcgtagtt gcggcccacg
    21661 cagtagacgc ggcgcaccgg gaacagatcg ttgctgccgg cgacgggcac gccgatgggg
    21721 gcttggggag cgaagacgta ggacatgatg actctgcgaa gtaggggcgc gccgggagcg
    21781 cgcgatttga aaacgaagga gcggccgaga tcaggccttg cgcaattcgc gccatgggtc
    21841 cagggcatcc tgcatcggcc tgcgcaaggc ggcgcagaag aggccgcgcg cgggccgaat
    21901 ggcaggtcct ggtcgatgag gcgcaccggg ccgagaggct ctgtctgtct cctgcaaatg
    21961 ccggtttgcc ggttgttttt agggggagcg aaggtcttgc cgggcagcct gcgcgagatg
    22021 tccggatcgc ttgatttgct ggaaattatg cgcatgcagc tatacgattt aaaatgaatt
    22081 aatcttatta ttcatacata aaaacatatg cctggaggcg gcatggccat ggattggacc
    22141 caccggctgc ggctgcgcaa cgtcaagatg ctgctcagcc tggcgcagac ccgcaatatc
    22201 agccattcgg cggccatgct caataccacc cagccgggcc tgtccaagtg gctgaaagac
    22261 ctggaagagg acatcgggct gccgctgttc gagcgccatg cgcgcgggct ggtccccacc
    22321 cactacggcg aggtgctgat cgagcatgcc cgccgcctgg acacgcagtt ggatcgcgcc
    22381 agccgcgaca tggcggccct gcgcgagggc agcgccggcc gcgtggtgat cggcgcctcg
    22441 ggcgcggcgg cctccgatac ggcgccgctg gccatgctgc gcctgctgga ccggatgccg
    22501 caggccagcg tgagcctggt cgagggcacg acggaccggc tgctggggca gctggcgcag
    22561 ggcgacctgg atctggtcat ggggcgggcg gggccggaac ggacggatcc ggcgatcgag
    22621 atggagtcgc tgtaccggga tcccatccgc ctggtggcgc ggccgcacca ccccttgttg
    22681 tcgcgcgaga ggctgtcctg ggaggatttg cgggcttacc ggtggatcat ctggccccag
    22741 ggcacgccgg tgcgcacggc gctgaacgag gcgctggccg aagcggggga ggccttgccg
    22801 cgcgatgcgg tggagacgaa ttcggtgacc agcaacctca ccctgctcaa caacagcgac
    22861 atggtcgggg tggcgtcgca tcgggcggcc gtgcggctgg cgcagatgaa taccttgcgg
    22921 atcctgccga tacggatgtc ggggttcggg gcggtggcgc tgtattggcg ccacgagagt
    22981 ttccggccgc aggcggtgca ggtggcgatg gattgcatcc gcgaggtggt ggcggagtat
    23041 gcggcggtgg agggtgttta gtcctgcggg gtgtagtgca gtttttgcat cggataccct
    23101 tgcgcgctgg cgacggacag ggcctggtcc agttcgggct tgggcaattg cggcgttcgc
    23161 gacaggatcc acagcgattt gcggttgacc gagccgatga ccgtccagcg atagtccggg
    23221 tccagtccca ccaccacata atcggccgag aacggccgca tgaaggtcac ttccatcctg
    23281 gtgttgtcgc tgccctgcag gggcgtggcg gtgccatacg cttcattggt cccgccgtcc
    23341 cgggtacggc agcggttctt gatggcgaac gtgccgtcgc cgttggccgt atattcggac
    23401 acggtatcgg ctacgcagtg aggcaggaaa aaacggggaa aattggcaat ttcgtaccag
    23461 gttcccacgt agcggttcag gtcgaccttc tggacggctt gcacgggcgc cgcgccggcg
    23521 gtggctgggg gagaaacgat gcacgccagc gtcagcggca ccgcggacac gaaacgtcgc
    23581 atggagacct cctgtaggca atggtcggaa aaccttccgg cagccacttc agcaccggct
    23641 gccttccgat catagcgccg aagccgggtc taaaggcctg gcccaacctt tggcgtgttc
    23701 gaccgcttgc cgccacctgg ccatccgcgc ctcgcgccgg tccgccgacc aagcgggctc
    23761 gaagcggcgc tcggcctgcc aatgcccggc aagctcatcc tgcgacgacc agaatccgac
    23821 cgccagcccc gccagcccag cggcgcccag ggcggtcgat tcgagcacgc gcgggcgcac
    23881 caccggcacg cccagcaagt cggcctgcat ctgcatgagc aggtcgttgc gcgccgcgcc
    23941 gccgtcggcg cgcagctcgg acagggcgat gccgctgtcg gcctgcatgc aggccagcag
    24001 ctcggcgc
//
LOCUS       DASWPO010000213        10069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_227099, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000213 DASWPO010000000
VERSION     DASWPO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227099"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="VGC69_11320"
     CDS             <1..154
                     /locus_tag="VGC69_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY0295912.1"
                     /translation="TEVHHQRAFTNLSVRDVEVDFVLQTRGPEHIQEVIAQLNAAGFA
                     ASNHDR"
     gene            complement(174..779)
                     /locus_tag="VGC69_11325"
     CDS             complement(174..779)
                     /locus_tag="VGC69_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0295913.1"
                     /translation="MPTNTEPSAPSPFIVLDACVLMSTVLRQLLLRVARAGVFQPVWT
                     ERIGEEWRRNAARLWGAPQEILDGLWDDMNRDFPGAMEADIQPYESVLRYSDPKDFHV
                     IAAGLARRARCGLQQVPVVQVMTWNLKDFNRSELRRQGLDVFSPDVMLARWWQAGPER
                     LAPAVGLVEQDFLALGREPAPLAAILHRERLYRLEKLAAAG"
     gene            complement(831..915)
                     /locus_tag="VGC69_11330"
     tRNA            complement(831..915)
                     /locus_tag="VGC69_11330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(879..881),aa:Leu,seq:taa)
     gene            1056..4001
                     /locus_tag="VGC69_11335"
     CDS             1056..4001
                     /locus_tag="VGC69_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0295914.1"
                     /translation="MITFNRLVLAWKHRQLATHLALVFGSTVLVAMLCIVFYVNHQAV
                     GIVQDASQTLFDHTANESRSQIDKSVSAVDSLLSTYAADPQGGDFGSASQRVFIQRLS
                     VSLKASPLLSAFYIGFDDGGFVLLRQLTSPVARQKLDAPDTARYFLEIVRTGAQAARM
                     ELTFLDDQLNTVGTAAAPATPYDPRTRGWYQAAIGSEASILTDPYWFFVTQERGVTLA
                     RRLASGHGVVGADLGLADLSQALQQMKSTKSSALMIVNPNGEVIASSEPIKPDTSGDP
                     GAMPSWPASSIAVDMLEAATGAADEQAQTRTIDKDGVTWVMRTVPLHAGPWTFRMVTA
                     TPANEMLAGARELVSTLVWLGVAMAIAAMVVIQIASKAVSRPLVQLAREAERMQRLQF
                     KPETDEAPTSVREIQTLSQSMRQAGCTIQRFIEIGQALSAERDPMRLMGRLLQETITI
                     AEAEGGLLLLTEDRGETLSVIDRNTLAEGIESRPIGRHDVDGHVLQALADKAVAHFDC
                     ARAVLPAFLSSLVDAKPLANDQVFRFSVVPLLNHSEEVIGGLLLAARIEAGMEMPDAN
                     LELVRALSGTAAMTIEMTLLLKARKSLLDAVIRMIANSIDAKSPYTSGHCQRVPLLTT
                     ALVHAASQSRSGPFEGFSLSQDEWEAVKVASWLHDCGKLTTAEYIVDKATKLETIYDR
                     LHEIRMRFELLKANAWTAYWRGVAEGGNPQTLAVVRDADLAALDDDFSFVAACNEGGE
                     AMAPQDIERLQEIGDRKWIRTLDDRIGTSREEQQRKNRMPTPALPVEERLLDDRVDHL
                     IEHYDSRLGTLENQADFSLKPLPYRLNLGERYNLSIGRGTLTAEERYEINRHITHTIL
                     MLQDLPLTGALRLVPEFAGGHHEKMDGTGYPNGLRRDDMSIVARIMAIADVFEALTAA
                     DRPYKKAKKLSEVVRIMGFMKRDRHLDPDLLDLFLTEGIWRDYAREFMSPELIDEPDV
                     ESVLAIKVA"
     gene            complement(4020..4796)
                     /locus_tag="VGC69_11340"
     CDS             complement(4020..4796)
                     /locus_tag="VGC69_11340"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0295915.1"
                     /translation="MNSLTQLAADLAGGAIKVVDLTQTLSPDFPALVLPPQFGQVWAF
                     KMETISQYDENGPGWYWNNFSCGEHTGTHFDAPVHWVSGKDHPDNTVDTIDAKKFIAP
                     AVVVDASSEVAGNRDWVLTVEFLQAWESKHGKIPAGAWVLFRTDWSLKTDPADFLSLR
                     EDGAHTPGPSQETVEWLIHQRNVLGFGVETINTDAGQAFAWPLPYPCHTLMHGNNRYG
                     LQCLKNLDQLPPTGTMIVAAPLKIQKGSGSPLRVLALVAG"
     gene            5011..5334
                     /locus_tag="VGC69_11345"
     CDS             5011..5334
                     /locus_tag="VGC69_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295916.1"
                     /translation="MSTEKTVEKAEALAGKGANAAEDAMDTVASKANTMSDYASLKRF
                     ASLGAIVVLASLGSGCSSVVGECGWLRSSCMYDGHYEPGEKDYAEQKAQQLNKEEAQK
                     IKSGF"
     gene            complement(5446..5535)
                     /locus_tag="VGC69_11350"
     tRNA            complement(5446..5535)
                     /locus_tag="VGC69_11350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5499..5501),aa:Ser,seq:tga)
     gene            complement(5618..6592)
                     /gene="hemC"
                     /locus_tag="VGC69_11355"
     CDS             complement(5618..6592)
                     /gene="hemC"
                     /locus_tag="VGC69_11355"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0295917.1"
                     /translation="MSKTYKIGTRASLMAVWQTEAIIEALQAQFPQHRFEMVTRPARA
                     DLDLKSRLGALGGKGGAFIGAMQEMMLRGEADMVMHSLKDLPGNEEYYADTRFSIGAC
                     LPRSNPCDALVLKQGLAADAVPAVIGTASVRRGAFLRRLYRHARIVPFRGSADKRIAR
                     LDSDTPMEFNYGGKTPPLDALVLAQSGLERIGLGNRSSRIFTPDEMCPAVGQGVVVAE
                     YATGNEEVRQLLAAVNHRETAYCYQAERALLRALNGHCDSPIGAHAWIEDKQLKLRGI
                     VVSLDGASAVEVFDATSNIDPVSLGLRAGQRLNKLGAQAIIDETRYAD"
     gene            6757..7512
                     /gene="tpiA"
                     /locus_tag="VGC69_11360"
     CDS             6757..7512
                     /gene="tpiA"
                     /locus_tag="VGC69_11360"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0295918.1"
                     /translation="MTHQVRARLVLGNWKMHGSLAENALLLAALRAADVANAAGSHCQ
                     IGVCVPFPYLAQAAQALQGSAVSWGGQDISAHDKGAYTGEVSAAMLNDFACRWALAGH
                     SERRAYHGETDQVVADKARAALRAGLTPVVCVGETLAERDAGQTLAVIQRQLAPVLAL
                     GTDALARMVLAYEPVWAIGTGRSATPEQAQEVHGAIRAELAALGAAQVQVLYGGSVKA
                     ANAASLFAMPDIDGALVGGAALVAEEFLRIAAA"
     gene            7532..7945
                     /gene="secG"
                     /locus_tag="VGC69_11365"
     CDS             7532..7945
                     /gene="secG"
                     /locus_tag="VGC69_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY0295919.1"
                     /translation="MSYTTILLVVQFLTALAIIILVLLQQGKGADMGSSFGSGSAGSL
                     FGASGAANFLSRTTKWAAVVFFASTIGLAYASHSIKAGGTPIDSGVMQGFGAAAPKGD
                     AVPAANGTAATPAAPGADAAKGDASIPNVPAAPAK"
     gene            8023..8107
                     /locus_tag="VGC69_11370"
     tRNA            8023..8107
                     /locus_tag="VGC69_11370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8057..8059,aa:Leu,seq:gag)
     gene            8305..9618
                     /locus_tag="VGC69_11375"
     CDS             8305..9618
                     /locus_tag="VGC69_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0295920.1"
                     /translation="MPKLAQQLTDKAIRAFKPQAKVYTRPDGNNLHLRITPSGAKSWQ
                     VRYLLPTGERGAVVIGDYPAMGVAEARVKTAEIHQAARTGVPIVGQRAANQAQRQTRT
                     EAEIAAQRAVEEARKHSFMVLSDKWLEAMKPGWADESYRKARYVVHTYLQPKIGTLDM
                     RTMRSKDVTTPLREMAAKVPSLAKKAVQYLNGIVTYCIHEGIREDDQVLVLDGVLPSH
                     RGGHIPAVTQEQGIGPLMRAIHAYEGFVVRCALLLAAWTTLRSGVVATARWSEIDLER
                     AEWSIPGLDDGFHETGKRKNRMKTGHAHVVSLPTQAVEMLREMHQFTGGTEYVFPSVG
                     KRKNPHLHRDALSAALRGMGFGGKHAPHGFRAMLRTVARVKMKIDPDVLEAQLAHAKK
                     DEIQSAYDRTMFDDERREVMQRWADYLDNQAAIGINNAVSLEQQA"
     gene            complement(9742..>10069)
                     /locus_tag="VGC69_11380"
     CDS             complement(9742..>10069)
                     /locus_tag="VGC69_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0295921.1"
                     /translation="MFGAMGNPDHDESIGVIHRALDAGINFIDTADVYSLGESEEIVG
                     QALKGRRADVVLATKFGLPMGAGNDTGGGSRRWIIREVEDSLRRLGTDYIDLYQLHRP
                     DHATAT"
BASE COUNT         1806 a         3152 c         3322 g         1789 t
ORIGIN      
        1 caccgaggtg catcaccagc gggccttcac caacctgtcg gtgcgcgatg tcgaggtgga
       61 cttcgtgctg cagacgcgcg ggcccgagca catccaggaa gtcatcgcgc agctcaacgc
      121 ggcgggtttc gccgccagca accacgaccg ctgacccgcc gggtggcccg ttttcagccg
      181 gccgccgcca gcttttccag gcggtagagg cgctcgcggt gcaggatggc ggccaaaggg
      241 gcgggctcgc gccccagcgc caggaagtcc tgttcgacca aacccacggc gggcgccaat
      301 cgctcgggcc cggcctgcca ccagcgcgcc agcatcacgt ccgggctgaa cacgtccagg
      361 ccctgccggc gcagttcgga acggttgaaa tccttcaggt tccaggtcat tacctgcacc
      421 acgggcacct gctgcagccc gcagcgcgcg cgccgtgcca ggccggccgc gatcacatgg
      481 aaatccttgg ggtcgctata gcgcagcacc gactcgtaag gctggatatc agcctccatg
      541 gcgccgggaa agtcccggtt catgtcgtcc cacaagccgt cgagaatttc ctgcggcgcc
      601 ccccacaggc gcgccgcgtt gcgccgccat tcctcgccga tgcgctccgt ccagacgggt
      661 tggaacacgc ccgcccgggc cacgcgcagc agcagttgcc gcagcaccgt ggacatcagc
      721 acgcaggcgt ccaggacgat gaagggggac ggggcggagg gttcggtatt cgtcggcatg
      781 cacaggccct tgcccgcggc agggagaaaa cctggggccg ccaggagggg tggtgcaccc
      841 agccggaatc gaaccggcac gcctcgcggc gagggatttt aagtcccttg cgtctaccaa
      901 tttcgccatg ggtgcctgtt ttgggcaggc tggtggcccc tgtttcggga ggcggcaact
      961 ataacatcga catcggtgcg aattttcgtt tcttcggtgt actctacgac gcagaattac
     1021 attcgttaac gctatgtttc gcgatagagt cgcagatgat cacgttcaat cggttggtat
     1081 tggcgtggaa acaccggcag ctggccacgc atcttgccct ggtgttcggt tcgacggtgc
     1141 tggtggccat gctgtgcatc gtcttttatg tgaatcacca ggcggtcggc atcgtccagg
     1201 atgcttccca gaccttgttc gaccacacgg caaacgaaag ccgcagccag atcgacaaga
     1261 gcgtcagcgc ggtagacagc ttgctgagca cgtacgccgc cgatccccag ggcggcgatt
     1321 tcggcagcgc ttcccagcgc gtgttcatcc agcggctgag cgtctcgctc aaggcctcgc
     1381 cgctgctgtc ggcgttctac atcggtttcg acgacggcgg cttcgtgctg ctgcgccaac
     1441 tgacctcgcc cgtcgcccgc cagaagctcg atgcgcccga cacggcgcgt tatttcctgg
     1501 aaatcgtgcg cacgggcgcc caggccgcga ggatggagct caccttcctc gacgaccaat
     1561 tgaataccgt cgggacggcg gccgcgccgg ccacccccta cgatccgcgc acgcgcggct
     1621 ggtaccaggc ggccatcggc agcgaggcgt ccatcctcac ggacccgtat tggtttttcg
     1681 tgacgcagga gcgcggcgtc acccttgcgc ggcgcctggc gtccggccat ggcgtggtgg
     1741 gggccgatct cgggctggct gatttgtcgc aggcgctgca gcagatgaaa agcaccaagt
     1801 cctcggcgct gatgatcgtc aatcccaatg gcgaggtcat cgccagcagc gagccgatca
     1861 agccggatac ctcgggcgat ccgggcgcaa tgccgtcatg gcccgcatcc tccatcgcgg
     1921 tggacatgct ggaagccgcc accggcgcgg cggacgagca ggcccagacc cgcacgatcg
     1981 acaaggacgg cgtcacgtgg gtgatgcgca cggtgccgct gcatgccggc ccctggactt
     2041 tccgcatggt cacggccacg cccgccaacg aaatgctggc cggcgcgcgc gaactggtct
     2101 cgacactggt ctggctgggc gtggccatgg ccatcgccgc gatggtcgtg atccagatcg
     2161 cctccaaggc ggtcagccgc cccctggtgc agctggcccg ggaggccgaa cgcatgcagc
     2221 gtctccagtt caagccggag acggacgagg cgcctacctc cgtgcgggag atccagacct
     2281 tgtcgcagtc catgcgccag gccggctgca ccattcagcg cttcatcgaa atcggccagg
     2341 cgctctcggc cgagcgcgat cccatgcggc tgatgggccg gctgctgcaa gagaccatca
     2401 ccatcgccga ggccgaaggc gggctcctgc tgctgaccga agaccggggc gagacgctgt
     2461 cggtcatcga ccggaacacc ctggccgaag gtatcgagtc ccggccgatc ggcaggcatg
     2521 acgtcgacgg gcatgtgctg caagcgctgg cggacaaggc ggtcgcgcac ttcgactgcg
     2581 cgcgggcggt gctgccggca ttcctctcga gcctggtcga tgcgaagccg ctggcgaatg
     2641 accaggtctt ccggttttcg gtggtcccgc tgctcaatca ctccgaggag gtgatcggcg
     2701 gcctgctcct ggcggcgcgg atcgaggcgg gcatggagat gccggacgcc aatctcgaac
     2761 tggtgcgcgc cttgtccggc acggcggcga tgaccatcga gatgacgctg ctgctcaagg
     2821 cgcgcaagtc gctgctcgat gccgtgatcc gcatgatcgc caattccatc gacgccaagt
     2881 ccccctatac cagcgggcac tgccagcgcg tgcccttgct gaccacggcg ctggtgcatg
     2941 ccgccagcca gtcccgtagc gggcctttcg agggcttctc gctctcgcag gacgaatggg
     3001 aagcggtcaa ggtcgcgagc tggctgcatg attgcggcaa gctgaccacc gccgaataca
     3061 tcgtcgacaa ggccaccaag ctcgaaacca tctacgaccg tcttcacgaa atccgtatgc
     3121 gcttcgagct gctcaaggcc aatgcctgga cggcctattg gcgcggcgtg gccgagggcg
     3181 ggaatccgca gacgctggcc gtcgtccgcg acgccgacct ggccgcgctg gacgacgatt
     3241 tctccttcgt ggcggcgtgc aacgaaggcg gcgaggcgat ggcgccccag gatatcgagc
     3301 gtttgcagga gatcggcgat cgcaaatgga tacgcacgct cgacgaccgc atcggtactt
     3361 cgcgcgaaga gcagcagcgc aagaaccgga tgcccacgcc ggcgctgccc gtcgaagaaa
     3421 ggctgctgga cgaccgggtc gatcacctca tcgagcatta cgacagccgg ctcggcacgc
     3481 tggaaaacca ggccgacttc agcctgaagc cgcttccata ccgcctgaac ctgggcgaac
     3541 gctacaacct ctcgataggc cggggcacgc tgacggccga agagcgctac gagatcaacc
     3601 ggcacattac ccacaccatc ctcatgctgc aggatctgcc gttgacgggc gccttgcggc
     3661 tggtgccgga attcgccggc gggcaccacg agaagatgga cggcacgggg tatcccaacg
     3721 gcctgcgccg cgacgacatg agcatcgtgg cgcgcatcat ggccatcgcc gacgtcttcg
     3781 aggcgctgac cgctgccgat cggccctata aaaaggccaa gaaactgagc gaggtggtgc
     3841 gcatcatggg gttcatgaag cgcgacaggc acctcgaccc cgacttgctc gatctcttcc
     3901 tgaccgaggg catctggcgg gactatgcgc gcgaattcat gagccccgaa ctgatcgacg
     3961 agccggatgt cgaatccgtg ctcgcgatca aggtggcctg agccgcgggg cgcaggggct
     4021 cagcccgcga ccagcgccag cacgcgcagc gggctgcccg agcctttctg gatcttcagc
     4081 ggcgcggcca cgatcatggt gccggtgggc ggcaattgat cgaggttctt caggcattgc
     4141 aggccgtagc ggttgttgcc gtgcatcagc gtgtggcagg ggtagggcag gggccaggca
     4201 aacgcctggc cggcgtcggt attgatggtt tccacgccga agcccagcac gttgcgctgg
     4261 tggatcagcc attccaccgt ttcctgcgaa gggccgggcg tgtgggcgcc gtcttcccgc
     4321 aggctgagga aatcggccgg gtcggtcttc agcgaccagt cggtgcgaaa cagcacccag
     4381 gcgccggcgg ggattttgcc gtgcttgctt tcccacgctt gcaggaactc gaccgtcagc
     4441 acccagtcgc ggttgccggc cacctcggaa ctggcatcga cgaccacggc gggagcgatg
     4501 aatttcttgg cgtcgatggt gtcgacggta ttgtcgggat ggtccttgcc gctcacccaa
     4561 tgcacggggg cgtcgaagtg cgtgccggtg tgttcgccgc acgagaagtt gttccagtac
     4621 cagccggggc cgttctcgtc gtactggctg atggtttcca tcttgaaggc ccatacctgg
     4681 ccgaactggg gcggcagcac cagggccggg aaatccggcg aaagcgtttg cgtgagatcg
     4741 acgaccttga tcgcgccgcc ggcaaggtcg gcggccagtt gggtaaggct gttcatatgg
     4801 tgtttcccct tattgtggct ggaatcaggc gcctgcccgg cacgctggcg ggtggcggag
     4861 atgattcaag cttaaagtat gtggggcgtg gcaggcaagg gagggaaatg ccgggggtag
     4921 ggggcaagcg gacgctggca tccctgtcat cccgtctgtc cgtctgcacg gtagactctg
     4981 gctgaaattt caggaaacaa caggaacccc atgagtaccg aaaaaacagt cgagaaagcc
     5041 gaggcgctgg ccggcaaagg cgcgaatgcc gcggaagacg caatggatac ggtagccagc
     5101 aaggcaaata cgatgagtga ttacgcgtct ctaaaaaggt ttgccagcct tggcgcgata
     5161 gtggtgctcg cctcgcttgg ctctggctgt tcctcggtgg tgggcgaatg cggctggttg
     5221 cggagtagct gcatgtacga cggccactac gagcctgggg aaaaggacta cgccgagcag
     5281 aaagcgcaac aactgaataa agaagaggcc cagaaaatca aatcgggttt ctagtccttg
     5341 gttcggatcc gcggcttgtc gggaagctcc gaacgagatc gggtcatcgc ctgccgcgcg
     5401 aggccagagg gccttgcgcc tagcgatcca gatcggcatg agcaatggcg gaagcggtga
     5461 gattcgaact cacggacggt ttaacccgtc gctggttttc aagaccagtg ccttaaacca
     5521 ctcggccacg cttccatcgg gaaggctgca taataatcca tcccgtcatg ccatcgggat
     5581 gggcggggca cggcgcagcg gccggcccgc gcggggctca gtccgcgtag cgcgtctcgt
     5641 cgatgatggc ctgcgcgccc agcttgttga gccgctggcc tgcgcgcaat cccagcgaga
     5701 ccgggtcgat gttgctggtg gcgtcgaaca cctcgacggc gctggcgccg tcgagcgaca
     5761 ccacgatgcc gcgcagcttg agctgcttgt cctcgatcca ggcatgcgcg ccgatgggcg
     5821 agtcgcagtg gccgttgagc gcgcgcagca aggcgcgctc ggcctggtag cagtacgcgg
     5881 tctcgcgatg gttgacggcg gccagcagtt gccgcacttc ttcgttgccg gtggcgtatt
     5941 cggccacgac cacgccctgg cccacggcgg ggcacatttc gtcgggcgtg aagatgcgcg
     6001 aggagcggtt gcccaggccg atgcgttcca ggccggactg ggccagcacc agggcgtcca
     6061 gcggcggcgt cttgccgccg tagttgaatt ccatgggggt gtcgctgtcc aggcgggcga
     6121 tgcgcttgtc ggccgagccg cggaaaggga cgatacgcgc gtggcggtac agccggcgca
     6181 ggaaggcccc gcgccttacc gaggcagtgc cgatcacggc ggggaccgcg tcggccgcca
     6241 gtccctgctt gagcaccagg gcgtcgcagg ggttgctgcg cggcaggcag gcgccgatgg
     6301 agaaccgggt gtcggcgtag tattcttcgt tgccgggcag gtccttgagc gagtgcatga
     6361 ccatgtcggc ctccccccgg agcatcatct cctgcatggc gccaatgaaa gcgccgccct
     6421 tgccgcccag ggcgcccagg cggctcttca ggtcgagatc ggctcgcgcc gggcgcgtga
     6481 ccatctcgaa gcggtgctgg ggaaactgcg cctgcagcgc ctcgatgatg gcctcggtct
     6541 gccacaccgc catcaggctg gcgcgggtgc cgattttgta agtcttggac atctggattc
     6601 ctgagtacgg aaatgcgccg ccggggcgca ggcgatcatc cgcctgccgc catgatagca
     6661 aggcctggaa atagggtcgg gcacgcttcg caccgacagg atacaatcac aggtttagct
     6721 cggactattt cgggctctga agtcccgtat cttgccatga ctcatcaagt tcgcgcccgg
     6781 ctggttctgg gcaactggaa aatgcacggc agccttgccg agaacgcgtt gcttctggcc
     6841 gcgttgcgcg cggccgatgt agccaatgcg gcgggctcgc actgccagat cggcgtctgc
     6901 gtgcctttcc cgtacctggc gcaggctgcc caggccttgc agggcagcgc cgtgtcctgg
     6961 gggggccagg acatcagcgc gcacgacaag ggcgcctaca cgggcgaagt ttcggccgcc
     7021 atgctcaacg attttgcctg ccgctgggcc ctggccgggc attccgagcg ccgtgcctat
     7081 catggcgaga ccgaccaggt cgtggccgac aaggcccgcg ccgccctgcg ggccggcctg
     7141 acgccggtcg tgtgcgtggg cgaaaccctg gccgagcgcg acgccgggca gacgctggcc
     7201 gtcatccagc gccaactggc tcccgtgctg gccctgggga cggatgccct ggcccgcatg
     7261 gtgctggcct atgagcccgt ctgggccatc ggcaccggcc gcagcgccac gcccgagcag
     7321 gcgcaagaag tccatggcgc catccgcgcc gagctggccg ccctgggcgc agcccaggtg
     7381 caggtgctct atggcggcag cgtcaaggcc gccaatgccg ccagcctgtt tgccatgccc
     7441 gacatcgacg gcgcgctggt tggcggcgcc gcgcttgtcg ccgaagaatt cctgcgcatc
     7501 gccgcagcct aagtttcgat ctggagtcat catgtcttac accaccatcc tgctggtcgt
     7561 tcagttcctg acggccctgg caatcatcat tctggtcctg ctgcagcagg gcaagggtgc
     7621 cgatatgggt tcgtccttcg gcagcggttc ggccggcagc ctgttcggcg catcgggcgc
     7681 ggccaacttc ctgtcgcgca ccaccaagtg ggccgccgtg gtcttcttcg cctcgaccat
     7741 cgggctggcc tatgcctcgc actccatcaa ggcgggcggc actcccatcg attccggcgt
     7801 catgcagggc ttcggcgccg ctgcgcccaa gggtgacgcg gtgcccgccg ccaacggcac
     7861 tgccgctact ccggcagcgc ctggcgcaga cgccgccaag ggcgatgcct ccattcccaa
     7921 cgtgcctgcc gcgcctgcga agtaagccga tcttgtgcta gaattttgga ctttccgcaa
     7981 aaagcaatag ctgcttttgc tttgcaggaa gcaaaaaccc aagccgtcgt ggtggaattg
     8041 gtagacacgc tatcttgagg gggtagtggc gaaagctgtg cgagttcgag tctcgccgac
     8101 ggcaccaaca cttagtccaa gctgtatcaa ggactgctag aaagcctgta agaatcaata
     8161 gcttacgggc ttttttgtcg cctgacgttc tgtggcgtgt tatggcgttg catagccttc
     8221 tagggttttt aggcaaattc ttgggtaaac ttttcgaggt ttacccaagg cgggctccgg
     8281 gtgaattcgt cctacgagag cgacatgcct aaacttgccc agcagcttac cgacaaagcc
     8341 atccgtgcct tcaagcccca ggccaaggtc tacaccaggc cggacggcaa caacctccac
     8401 cttcgcataa cccccagcgg ggccaagtcc tggcaagtgc gctatttgct gcccaccggc
     8461 gagcgtggtg cggtggtgat cggagactat ccggccatgg gcgtggccga ggcccgcgtc
     8521 aaaacggccg agatccacca ggcggcacga accggtgtcc ctatcgtcgg gcagcgtgcc
     8581 gcgaatcagg ctcagcggca aacccgtaca gaagccgaga tcgcagccca gcgggccgtc
     8641 gaggaggcgc gtaagcattc gttcatggtg ctgtcggaca aatggctgga ggctatgaaa
     8701 cccggctggg ccgacgagag ctaccgcaag gcccgatatg tcgtgcacac ctacttgcag
     8761 cccaagattg gcaccttgga catgcgcaca atgcgcagca aggacgtgac cacgcctctc
     8821 cgagaaatgg cggccaaggt tcccagtctg gcgaagaagg cggttcagta cctaaacggg
     8881 atagtgacct attgcatcca cgaaggtatc cgcgaagacg atcaagtcct ggttctggat
     8941 ggggtgctgc ctagtcacag gggcggccat atcccggcgg taacccagga acagggtatc
     9001 ggcccgctca tgcgcgctat ccacgcctat gaagggttcg tggtgcgctg tgcgctattg
     9061 ctggcggcgt ggacgacgct gcgatctggc gtggtggcta cggcaagatg gtcggagatc
     9121 gacttggagc gagccgagtg gtccattccc ggcctggatg atgggtttca cgaaacgggt
     9181 aagcgtaaaa accgcatgaa gaccgggcat gcccatgtcg tcagtctgcc gactcaggcg
     9241 gtggaaatgt tgcgagaaat gcaccaattc accggaggga ccgagtacgt attcccgtct
     9301 gtagggaaga ggaagaatcc gcacctgcat cgtgacgcgc tgtcggcggc attgaggggt
     9361 atgggatttg gcgggaagca tgccccgcat gggttccgag ccatgttacg cactgtggca
     9421 cgggtgaaga tgaagatcga tcctgacgtg ctggaagctc agcttgccca tgccaagaag
     9481 gatgaaattc aatcagccta tgatcggact atgttcgatg atgagcggcg cgaggtaatg
     9541 cagagatggg ctgactatct ggacaatcag gctgctattg gcattaacaa cgcggtttca
     9601 ctggaacagc aggcataatc ttttacgctt gcaccttggc ccgagagggt gagatgccgg
     9661 aacgtctacc ggattggtgc tggttcctac tttaaatagg cggcgcatag gtgagcgcat
     9721 ggagatggta gatcttagcc attacgtcgc ggtggcgtgg tccggccggt gcagctggta
     9781 caggtcgatg tagtcggtgc cgaggcgacg caggctgtcc tcgacctccc ggatgatcca
     9841 gcgcctcgac ccgccgcccg tgtcgttgcc cgcgcccatc ggaagcccga acttggtggc
     9901 gagcacgaca tcggcgcgcc gccccttcag cgcttggccg acgatctcct cggactcccc
     9961 gagggagtac acgtctgccg tgtcgatgaa gttgatcccg gcgtccagcg cgcggtggat
    10021 gacgccgatc gactcatcgt ggtccgggtt gcccattgca ccgaacatc
//
LOCUS       DASWPO010000214        15231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2370088, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000214 DASWPO010000000
VERSION     DASWPO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370088"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1357
                     /gene="hisD"
                     /locus_tag="VGC69_11385"
     CDS             59..1357
                     /gene="hisD"
                     /locus_tag="VGC69_11385"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0295922.1"
                     /translation="MPLINRLDSRDPGFKAALAKLLAFEAGEDESIDRAAAGILADVR
                     KQGDAALLDYTRRFDRVQADSAAALEIPKAEWQAALAALPAAQRAALEAAADRVRRYH
                     ERQRGETWTYTEADGTVLGQKITPLDRVGLYVPGGKAAYPSSVLMNAIPAKVAGVPEV
                     IMVTPTPDGVRNPIVLAAAAIAGVDRAFAVGGAQAVGALAYGTATVPAVDKIVGPGNA
                     YVAAAKRRVFGVVGIDMIAGPSEILVICDGSTPADWIAMDLFSQAEHDELAQAILLCP
                     DAAYIRQVEAAIERLLPQMPRLDILRTSLGNRGALIQVRDLDEACAIANDIAPEHLEI
                     STENPQQWAARIRHAGAIFMGRYASEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQK
                     RSSLIQVSRQGAQTLGRVAVELAMGEGLQAHAASARYRLD"
     gene            1367..1954
                     /gene="hisB"
                     /locus_tag="VGC69_11390"
     CDS             1367..1954
                     /gene="hisB"
                     /locus_tag="VGC69_11390"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY0295923.1"
                     /translation="MRTAEITRNTNETRIRVAINLDGTGKQSIATGVPFLDHMLDQIA
                     RHGLIDLDIQAEGDLHIDDHHTVEDVGITLGMAVAQAVGSKAGLRRYGHAYVPLDEAL
                     SRVVIDFSGRPGLVFNLDFTRARIGSFDVDLTREFFQGFVNHALVTLHVDNLRGINAH
                     HQCETVFKAFGRALRMAAELDPRLDGAVPSTKGVL"
     gene            2016..2705
                     /locus_tag="VGC69_11395"
     CDS             2016..2705
                     /locus_tag="VGC69_11395"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY0295924.1"
                     /translation="MSTIAIVDYGMGNFHSVARALAHAAPDADIRICNQPGQIDAADR
                     VVFPGQGAMADCMRTLNESGLREAVLRAAREKPLLGVCVGEQMLFEHSEEGDTPGLGI
                     FPGTVRRFAGPQFAAPVAGDGAAPQGGPAAAGSGDERLKVPHMGWNRVRQTRSHPLWR
                     DIPDGTHFYFVHSYYADPADAGLTTGVSDYGLAFTCAVAAANIFAVQFHPEKSAEHGL
                     RLYRNFVDWNP"
     gene            2800..3561
                     /gene="hisA"
                     /locus_tag="VGC69_11400"
     CDS             2800..3561
                     /gene="hisA"
                     /locus_tag="VGC69_11400"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY0295925.1"
                     /translation="MLLIPAIDLKDGRCVRLRQGDLDDATVFSEEPATMASHWLELGA
                     RRLHLVDLNGAVAGKPANGAAIQDILDAVGDEIPVQIGGGIRDLDTIERYLDAGISYV
                     IIGTAAVKNPGFLHDACGAFPGSIIVGLDARDGKVATDGWSKLTRHDVLDLAKKFEDY
                     GCEAIIYTDIGRDGMLSGVNVDATVRLAQHVRIPVFASGGIAGLTDIEALCAVEDEGV
                     EGAILGRSIYEGTLDFQAAQARADELNGIDPGDNE"
     gene            3680..4459
                     /gene="hisF"
                     /locus_tag="VGC69_11405"
     CDS             3680..4459
                     /gene="hisF"
                     /locus_tag="VGC69_11405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY0295926.1"
                     /translation="MTRRIIPCLDVTAGRVVKGVNFVNLTDAGDPVEIARRYDEQGAD
                     ELTFLDITATSDGRDLILPIIEQVASQVFIPLTVGGGVRQVADIQRLLNAGADKISIN
                     SAAVANPELVRAASDYHGSQCIVVAIDARRVAGPGPAEDEPGRWEVYTHGGRKPTGLD
                     AVAWARRMAAYGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGNLQHM
                     ADGVTLGRASAVLAASIFHFGQHSVRECKQYMAERGISVRL"
     gene            4492..4932
                     /gene="hisI"
                     /locus_tag="VGC69_11410"
     CDS             4492..4932
                     /gene="hisI"
                     /locus_tag="VGC69_11410"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY0295927.1"
                     /translation="MCDTHGKAPAPADTSLPAWLADVAFDAHGLIPAIAQDAATGQVL
                     MVAWMNREALLETANTGRAVYWSRSRKRLWRKGEESGHEQPVHELRLDCDGDVILLKI
                     EQIGGIACHTGRASCFYRRLDGQPGTAVWTTVDPVLKDPELIYK"
     gene            4929..5273
                     /locus_tag="VGC69_11415"
     CDS             4929..5273
                     /locus_tag="VGC69_11415"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY0295928.1"
                     /translation="MTTPAPASDLLARLADRLATRRPDQGGDPQTSYVAKLLSKGPDA
                     FLKKIGEEATELVMAAKDGLPERIVSETADLWFHCLVLLTHYNLRPEDVLAELARREG
                     LSGLEEKARRPE"
     gene            5286..5654
                     /locus_tag="VGC69_11420"
     CDS             5286..5654
                     /locus_tag="VGC69_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY0295929.1"
                     /translation="MSENCIFCKIVKGEIPSKRVYVDEDCVVFHDINPAAPVHLLVVP
                     RKHVVSLQDVTPADAGWLGRMMALVPQLASENGCRPGPEGGFRLLVNSGKDGGQEVAH
                     LHFHILGGPRPWQGRVAPAA"
     gene            5667..5882
                     /gene="tatA"
                     /locus_tag="VGC69_11425"
     CDS             5667..5882
                     /gene="tatA"
                     /locus_tag="VGC69_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEY0295930.1"
                     /translation="MGSFSIWHWLIVLVIVALIFGTKKLRNIGGDLGGAVKGFKEGMK
                     DAAAEQVTQKSVTGETIDVQAKEKTQA"
     gene            5882..6391
                     /gene="tatB"
                     /locus_tag="VGC69_11430"
     CDS             5882..6391
                     /gene="tatB"
                     /locus_tag="VGC69_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY0295931.1"
                     /translation="MFDISFSELAIIGVVALVVIGPERLPKVARTVGHLMGRAQRYVN
                     DVKSDIQREIELDEFRNLKQSVDNAASELRTSLQDTENTIRTAGQSLQAELAATTSEA
                     KQALDLHAQVDAASSEASAQAAAPAEPATQAELPLADATPTPLPTPAPTAPAQAPAPS
                     ANATTGTPT"
     gene            6388..7185
                     /gene="tatC"
                     /locus_tag="VGC69_11435"
     CDS             6388..7185
                     /gene="tatC"
                     /locus_tag="VGC69_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY0295932.1"
                     /translation="MSNTVAPEEKPQQEESFISHLIELRSRLIKAGAAVLVVFLVLFI
                     YPGASFIYDLLAQPMLAALPQGTHMIATGVITPFMVPVKVTMLASLVIALPIVLYQAW
                     AFVAPGLYRHEKRLALPLIVSSTLLFILGMAFCYFFVFRTVFHFIANFAPQSITPAPD
                     IEAYLSFVMTMFLAFGVTFEVPVVVVLLVKTGIVPLAKLRAARGYVVVGAFIVAAVVT
                     PPDMVSQCLLAIPLCLLYELGLQVARLVEPPAKPEDTPDSDAVVERD"
     gene            7278..7697
                     /locus_tag="VGC69_11440"
     CDS             7278..7697
                     /locus_tag="VGC69_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0295933.1"
                     /translation="MTLANRLRARMRWRGIRSQTQLARLSGVSQSSIHHILTRGDGYA
                     AKRETLRRLAAALGTTPAWLDGDTGEPPALREPHPAYAADGYDAELQALMARLPGPAR
                     RKIVDIVRLIAENATPTEQTQEEKPVGDAATDDKPDP"
     gene            complement(7720..8931)
                     /locus_tag="VGC69_11445"
     CDS             complement(7720..8931)
                     /locus_tag="VGC69_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0295934.1"
                     /translation="MRRLWLIFAQAVTICLAILFVVTTLRPDWLRLAGPYRPMPPQAG
                     AVPVPTPAPLQGDPVPPRAPPAAGRGVVSYADAAARAVPWVLNIYTTKHVDVPLVPLP
                     DDPTLQHLLGQVPGMSRRQPASSLGSGVIMREDGYVLTNYHVVEAADAIEVALTDGRK
                     SAAKVVGVDPETDLAVLKLAVGLGRLPVAALAPEHALRVGDVVLAIGNPFGVGLTTTQ
                     GIVSALGRNGLGINTFENFIQTDAAINPGNSGGALIDARGQLVGINTAIYSETGGSLG
                     IGFAIPIESARRVMDEIIRSGAVQRGWLGVEPQDITPDLAQAFQLPTGVSGVILASVV
                     HDGPAGRAGLKVGDIVQRLAGTPVLNTPGFLSMIASLPPGRETTLDVLRGSKAMQVKV
                     KVVSRPAGQEK"
     gene            8954..9730
                     /locus_tag="VGC69_11450"
     CDS             8954..9730
                     /locus_tag="VGC69_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY0295935.1"
                     /translation="MKTIATRELAAWLDDTLQAPRFKDYCPNGLQVEGRERVGTIIAG
                     VTACEALLRAAIERGADAVLVHHGWFWKNEDPRVRGPRRTRLALALAHDINLFAYHLP
                     LDAHPRLGNNAQLARVLGLAPDTDADGRPRTCGPDGLVWLGTAPGLATLGRLAERVAQ
                     RLNRTPLVVGDADQPIGRVAWCTGGAQGFLDAAIEAGADAYLTGEASEPTVHLARETG
                     TGFLAAGHHATERYGVQALGCAVAERFGIEVEFVDIDNPV"
     gene            9880..11178
                     /locus_tag="VGC69_11455"
     CDS             9880..11178
                     /locus_tag="VGC69_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEY0295936.1"
                     /translation="MVGLTPDIKTRILQAVDQGYAAQTDLLARLVRIPSQRGGEAEAQ
                     RFMAGQYAARGYEVDHWRIDVDAIRDMPGFSPVAVSYDDAYNVVGTHRSRTRAGRSLI
                     LNGHIDVVPVGPLAQWSRDPYDPAIVDGWMHGRGAGDMKSGLIACMAALDALRGLGCQ
                     PAADVFLQSVVEEECTGNGALACLQRGYRADAAFIPEPFTPMLMRAQVGPMWFQVQVD
                     GDPQHASAAFSEAGANAIEKAIFLIQAMGELEEKWNGRKGQHAHFHDHPHPIRFNLGK
                     IAGGDWPSSVPAWCTFDMRVAVFPGQSLETARAEIEAFVAEVAARDPFLAKHPPKVVY
                     HGFMAEGYELKNADEAEAALRASHEQVFNEPLREHATSAATDARFYGLYADTPAIVYG
                     PECRMPHGYDEAVNLESVRKVTQTIALFIAEWCGLEEIAA"
     gene            11175..12254
                     /locus_tag="VGC69_11460"
     CDS             11175..12254
                     /locus_tag="VGC69_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0295937.1"
                     /translation="MTTRSNAATSEQALFAADPPKLSTAEARSLAEQVYGLAAQVQLL
                     SSERDQNFQLRLDDGRAFVLKVTHPAEDPAVTDFQTQAQLHVMRADGALPVPHLFPSR
                     DGAYVHWHEGPDGTRRAVRLMAFLEGVPLYKAARSAGQGAAIGHALASFDLALADFDH
                     PMAGHELLWDLQHADRLADLLARIGEADRRALAERHMERFRSRVRPALAGLRRQVIHN
                     DLNPYNMMVHPRDPARVAAILDFGDMVRAPLAQDLAVACAYRLEDEGELLSQATPLVA
                     SYHRALPLAETEIALLPDLIAARLLITVAITGWRAEQHPENRTYILRNNGLAWSGLAR
                     LDALPLSQVQDVLFNACRTTLENPA"
     gene            12251..13579
                     /locus_tag="VGC69_11465"
     CDS             12251..13579
                     /locus_tag="VGC69_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0295938.1"
                     /translation="MSKDKAMLNAFDPAAAGELPAAERAMIARRQRVLGPAYRLFYDK
                     PLHIVRGEGVWLYGADGQRYLDAYNNVASVGHSHPRVVAAIAEQAAVLNTHTRYLHDG
                     VLDYAERLLATLPEPLSQVIFTCTGSEANDLALRVARSCTGASGVIVTQLAYHGVTAS
                     VAAVSPSLGKSVPLGLETRTVAAPDTYRLDPATLGAWFAAQVQSAIDDMLRHGIRPAA
                     LLVDTIFSSDGVYADPAGFLAPAVEAIRAAGGLFIADEVQAGFARTGSRMWGFQRHGL
                     VPDIVTMGKPMGNGHPIAAMVARPDVLERFGRESRYFNTFGGNPVSCAAAAATLSVIQ
                     DEGLQANAARTGQYLREGFRQLAQRHERIGDVRGDGLFIGVELVQDRASKAPAKEAMH
                     GFVNRMREERILLSATGLHGNVLKLRPQLPFGREHADILLAAADKVLAGL"
     gene            complement(13623..15104)
                     /locus_tag="VGC69_11470"
     CDS             complement(13623..15104)
                     /locus_tag="VGC69_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0295939.1"
                     /translation="MKEVLLSDLLAGALSREAEQSLQRQIYEIVRRGVLDHTLVAGQR
                     LPSSRALADELGISRITVSLAYDRLIAESYLTARSGSGTFVERTVERPLAGISQAEIA
                     RQRPGISTRGAVIGSGTGGLGQHSGAFVPGIADAALFPFHIWRRLLARHLDKSNLALA
                     GYAKDGAGFLPLRETIANYLRISRSVVCEPSQVIITAGTHQSVDLCARLLADAGDAAL
                     VESPCHWAFPPVLAAAGLGVRAAGLDEHGVTLAPGRIPRRTRMLITSPSHQYPTGVVM
                     PLSRRLELIQAARDRALWIIEDDYDSEFRYDGAPIPSLQGLDTDGRVIYMGTFSKTLF
                     AGLRMSYMVVPGHVAHAFSNACARIYRPGHLHLQAALADFMADGHFSHNIKRMRVEYA
                     ERQGVLREALHAKAGDLMTLSEARAGLHVYARLAPRVPAARLAEAARAEGLVLGMPHF
                     LPDHDGRDRNAVVLGYGGVPTAHIADGVDRLARALARVAESKR"
BASE COUNT         2472 a         5673 c         4899 g         2187 t
ORIGIN      
        1 tcgcgccgcg gcccctgggt ccgccctcgc aatgcggctt aagaacccaa ataccgccat
       61 gcctctcatc aaccgcctgg actcccgcga ccccggcttc aaggccgccc tcgccaagct
      121 gctggccttc gaagccggcg aagacgaatc catcgaccgc gccgcggccg gcatcctggc
      181 cgacgtgcgc aagcagggcg atgccgcgct gctggactac acccgccgtt tcgaccgcgt
      241 gcaagccgat tccgccgccg ccctggagat ccccaaggcc gaatggcagg ccgcgctcgc
      301 cgccctgccc gccgcccagc gcgcggccct ggaggccgcc gccgaccgcg tccgccgcta
      361 ccacgagcgc cagcgcggcg agacctggac ctacaccgaa gccgacggca ccgtgctggg
      421 ccagaaaatc acgccgctcg accgcgtggg cctgtacgtg cccggcggca aggccgccta
      481 tccgtcctcg gtgctgatga acgccatccc ggccaaggtg gccggcgtgc ccgaagtcat
      541 catggtcacg cccacgcccg acggcgtgcg caaccccatc gtgctggccg ccgccgccat
      601 cgccggcgtg gaccgcgcct tcgccgtggg cggcgcccag gccgtgggcg ccctggccta
      661 cggcaccgcc acggtgcccg ccgtggacaa gatcgtcggc cccggcaatg cctacgtggc
      721 ggccgccaag cgccgcgtgt tcggcgtggt gggcatcgac atgatcgccg gccccagcga
      781 aatcctggtg atctgcgacg gcagcacgcc ggccgactgg atcgccatgg acctcttctc
      841 gcaggccgag cacgacgagc tggcccaggc catcctgctg tgcccggacg ccgcctacat
      901 acggcaagtc gaggccgcga tcgaacggct cctgccgcag atgccgcgcc tggacatcct
      961 gcgcacgagc ctgggcaacc gcggcgcgct catccaggtg cgcgacctgg acgaggcctg
     1021 cgcgatcgcc aacgacatcg cccccgagca cctggaaatc tccaccgaaa acccgcaaca
     1081 atgggccgcc aggatccgcc acgccggcgc catcttcatg ggccgctacg cctccgaggc
     1141 gctgggcgac tactgcgccg gccccaacca cgtgctgccc acctcgcgca ccgcgcgctt
     1201 ctcctcgccg ctgggcgtgt acgatttcca gaagcgctcc agcctgatcc aggtatcccg
     1261 gcaaggcgcc cagaccctgg gccgcgtggc cgtcgaactg gccatgggcg aggggctgca
     1321 ggcccacgcc gccagcgccc gctaccgtct ggactaaaat ttcgccatgc gcaccgccga
     1381 aatcacccgc aataccaacg aaacacgcat ccgcgtcgcg atcaacctcg acggcaccgg
     1441 caagcagagc attgccaccg gcgtgccctt cctcgaccac atgctcgacc agatcgcccg
     1501 ccacggcctg atcgacctcg acatccaggc cgagggcgac ctgcacatcg acgaccacca
     1561 caccgtcgag gacgtcggca tcacgctggg catggccgtc gcccaggccg tgggcagcaa
     1621 ggccggcctg cgccgctacg gccacgccta cgtgccgctc gacgaggccc tgtcgcgcgt
     1681 ggtgatcgat ttctccggcc ggcccggcct ggtgttcaac ctggacttca cgcgcgcgcg
     1741 catcggcagc ttcgacgtcg acctcacccg cgaattcttc cagggcttcg tcaaccacgc
     1801 cctggtcacg ctgcacgtcg acaacctgcg cggcatcaat gcgcaccacc agtgcgaaac
     1861 cgtcttcaag gccttcggcc gcgcgctgcg catggccgcc gagctcgatc cgcgcctgga
     1921 cggcgccgta ccgtcgacca agggcgtcct gtaaacgcac cctgcggcgc ctcgcgccgc
     1981 gtccactcct gccgcgatca caccccgtga aacccgtgag taccatcgcc atcgtcgact
     2041 acggaatggg caacttccat tccgtcgcgc gcgccctggc gcacgccgcc ccggatgccg
     2101 acatccgcat ctgcaaccag cccggccaga tcgacgcggc cgaccgcgtc gtcttccccg
     2161 gccagggcgc catggccgac tgcatgcgca cgctcaacga gtcgggcctg cgcgaggcgg
     2221 tgctgcgcgc cgcgcgcgag aagcccctgc tgggcgtctg cgtgggcgaa cagatgctgt
     2281 tcgagcacag cgaagaaggc gacacgcccg gcctggggat ctttcccggc acggtgcgcc
     2341 gcttcgccgg cccgcagttc gccgcgccgg tggcgggcga cggcgccgcc ccgcaaggcg
     2401 gcccggcggc ggccgggtcc ggcgacgaac gcctgaaagt gccgcacatg ggctggaacc
     2461 gcgtgcgcca gacccgttcg caccccctct ggcgcgacat tcccgacggc acgcacttct
     2521 acttcgtgca cagctattac gccgaccctg ccgatgccgg cttgaccacg ggggtaagcg
     2581 attatggcct cgcctttacc tgcgcggtgg ccgccgctaa tattttcgcg gtgcagttcc
     2641 acccggagaa aagcgccgag cacggtttgc ggctctaccg caattttgta gactggaacc
     2701 cctgagcccc caagggctcg tccgcgagct cgcccgccac gtcatggcac ggccgctttt
     2761 atccattccg acacttacac aagacgcacg ccacccacca tgctgctgat ccccgccatc
     2821 gacctcaaag acgggcgctg cgtgcgcctg cgccagggtg acctggacga tgccacggtg
     2881 ttctccgaag aacccgccac catggccagc cactggctgg aactgggcgc gcggcgcctg
     2941 cacctggtcg acctcaacgg cgccgtcgcc ggcaagcccg ccaacggcgc cgccatccag
     3001 gacatcctcg acgccgtggg cgacgagatc ccggtgcaga tcggcggcgg catccgcgat
     3061 ctcgacacca tcgagcgcta tctcgacgcc ggcatctcct acgtcatcat cggcaccgcc
     3121 gcggtcaaga acccgggctt cctgcacgac gcctgcggcg ccttccccgg cagcatcatc
     3181 gtcggcctgg acgcgcgcga cggcaaggtg gccaccgacg gctggagcaa gctcacccgc
     3241 cacgacgtgc tcgacctcgc caagaagttc gaggactacg gctgcgaggc catcatctac
     3301 accgacatcg gccgcgacgg catgctctcg ggcgtgaacg tcgacgccac cgtgcgcctg
     3361 gcgcagcacg tgcgcatccc cgtgttcgcc tccggcggca tcgccggcct gaccgacatc
     3421 gaagccctgt gcgcggtcga ggacgaaggc gtcgaaggcg ccatcctggg ccgcagcatc
     3481 tacgaaggca cgctcgactt ccaggccgcg caggcccggg ccgacgagct gaacggcatt
     3541 gaccccggcg acaatgaata acggcacagc cagcgctccc cgacgcccct ccccccctcc
     3601 cggagggggg gaccaagggg ggggagccca catccgcccc aaaggggatg atgcgcctcg
     3661 tcaggccgca acaagcggcc tgacgaggcg catcatccct tgcctggacg tcaccgccgg
     3721 gcgcgtggtc aagggcgtga acttcgtcaa cctcaccgat gccggcgatc ccgtcgagat
     3781 cgcgcgccgc tacgacgagc agggcgccga cgaactcacc ttcctcgaca tcaccgccac
     3841 cagcgacggc cgcgacctga tcctgcccat catcgagcag gtggcctcgc aggtcttcat
     3901 cccgctcacc gtgggcggcg gcgtgcggca ggtagccgac atccagcgcc tgctcaacgc
     3961 cggcgccgac aagatcagca tcaacagcgc ggccgtggcc aaccccgagc tggtgcgcgc
     4021 cgccagcgac taccacggct cgcagtgcat cgtggtggcc atcgacgcac gccgcgtggc
     4081 cggccccggc ccggccgagg acgagcccgg gcgctgggag gtctacaccc acggcgggcg
     4141 caagcccacg ggcctggacg ccgtggcctg ggcgcgccgc atggcggcct acggcgccgg
     4201 cgagatcctg ctcaccagca tggaccgcga cggcaccaag gccggcttcg acctggcgct
     4261 cacgcgcgcg gtgtccgacg ccgtgccggt gcccgtgatc gcctccggcg gcgtgggcaa
     4321 tctgcagcac atggccgacg gcgtcaccct cggccgtgcc agcgccgtgc tggcggccag
     4381 catcttccac ttcggccagc actccgtgcg cgagtgcaag caatacatgg ccgagcgcgg
     4441 catctccgtg cggctgtagg ccccttccac ccggccagcg agaccctcat catgtgcgac
     4501 actcacggca aagcccccgc ccccgctgac accagcctgc ccgcctggct ggccgacgtc
     4561 gccttcgacg cgcacggcct catccccgcc atcgcccagg acgccgccac cggccaggtg
     4621 ctgatggtgg cctggatgaa ccgcgaggcc ctgctcgaga ccgccaacac cggccgcgcc
     4681 gtgtactggt cgcgctcgcg caagcgccta tggcgcaagg gcgaggaatc cggccacgag
     4741 caaccggtgc acgaactgcg cctggactgc gacggcgacg tcatcctgct gaaaatcgag
     4801 cagatcggcg gcatcgcctg ccacacgggc cgggccagct gcttctaccg ccgtctggac
     4861 ggccagcctg gcacggccgt ctggacgacc gtcgacccgg tgctcaaaga ccccgaactg
     4921 atctacaaat gaccactccc gcccccgcca gcgacctgct ggcccgcctg gccgacaggc
     4981 tggccacgcg ccgccccgac cagggcggcg acccgcagac ctcctatgtg gccaagctgc
     5041 tgtccaaggg gcccgacgcc ttcctgaaaa agatcggcga agaagccacc gagctcgtca
     5101 tggccgccaa ggacgggctg cccgagcgca tcgtcagcga aaccgccgac ctgtggtttc
     5161 attgtttggt cttgctcacg cactacaatc tgcggcctga agacgtgctg gccgagctgg
     5221 cccgccgcga gggcctttcc ggcctcgaag aaaaagcccg ccgtcccgag taagatgagg
     5281 cttccatgag cgaaaactgt atcttctgca agatcgtcaa aggcgagatc ccgtccaaga
     5341 gggtctacgt ggacgaggat tgcgtggttt tccacgacat caacccggcc gcgccggtgc
     5401 atctgttggt ggttccgcgc aaacacgtgg tttccctgca ggatgtcact cccgccgatg
     5461 cgggctggtt aggtagaatg atggcattgg ttccgcagct ggcttccgag aacggttgcc
     5521 gccccggacc cgaaggcggg ttccgcctgc tcgtcaattc cggcaaggat ggcggccagg
     5581 aagtcgccca cctgcatttc cacatacttg gcggtccgcg tccatggcaa ggccgtgtgg
     5641 cgccggcagc ttaacggagg ctctccatgg gcagtttcag catttggcat tggttgatcg
     5701 ttctggtgat cgtggcgctg atcttcggca ccaagaagct gcgcaacatc ggcggcgacc
     5761 tgggcggcgc cgtcaagggc ttcaaggaag gcatgaagga cgccgccgcg gagcaggtca
     5821 cgcaaaagag cgtcactggc gagaccattg acgtccaggc caaagagaaa actcaggcct
     5881 gatgtttgac atcagcttct ccgaactggc gatcatcggc gtcgttgcgc tggtggtcat
     5941 cggtcccgaa cgcctgccca aggtggcccg cactgtcggc cacctgatgg gtcgcgcgca
     6001 gcgttacgtc aacgacgtca agtccgacat ccagcgcgag atcgaactcg acgagttccg
     6061 caatctcaag caaagcgtcg acaacgccgc cagcgagctg cgcacctcgc tgcaggacac
     6121 cgaaaacacc atccgcaccg ccgggcaatc cctgcaggcc gagctcgccg ccaccaccag
     6181 cgaggccaag caggcgctcg acctccacgc gcaggtcgac gcagcctcgt ccgaggcgtc
     6241 cgcgcaggca gccgcccccg ccgagccggc gacccaggcc gaattgccgc tggccgacgc
     6301 cacgcccaca cccctaccca cacccgcgcc tacggcgccg gcacaagcgc ccgcgccgtc
     6361 cgccaacgcc accacgggta cgcccacgtg agcaacaccg tagcccccga agaaaaaccg
     6421 cagcaagaag aaagcttcat ctcccacctc atcgaactcc gctcccgcct gatcaaggcc
     6481 ggcgccgcgg tgctggtggt gttcctggtg ctcttcatct atcccggcgc ttcgttcatc
     6541 tacgacttgc tggcccagcc catgctggcc gcgctgccgc agggcacgca catgatcgcc
     6601 accggcgtca tcacgccctt catggtgccg gtcaaggtca ccatgctggc ctccctggta
     6661 atcgccctgc ccatcgtgct ctaccaggcc tgggccttcg tcgcccccgg cctgtaccgc
     6721 cacgaaaagc gcctggcgct gccgctcatc gtctccagca cgctgctctt catcctgggc
     6781 atggcgttct gctatttctt cgtgttccgc acggtcttcc acttcatcgc caacttcgcc
     6841 ccgcaatcga tcacgccggc gcccgacatc gaagcctatc tcagcttcgt catgaccatg
     6901 ttcctggcct tcggcgtcac cttcgaggtg ccggtggtcg tggtgctgct ggtcaagacg
     6961 ggcatcgtgc cgctggccaa gctgcgcgcc gcgcgcggct atgtggtcgt gggcgccttc
     7021 atcgtggccg ccgtggtcac gccgcccgac atggtcagcc agtgcctgct ggccattccc
     7081 ctgtgcctgc tctacgagct gggcctgcag gtcgcccgcc tggtggagcc gcccgccaag
     7141 ccggaagata cgccggactc cgacgccgtc gtcgagcgcg actgagtccc gcgccgccgg
     7201 cggtgcgggt attcccgctg tcataaattg gacgctttca ccttgttcag gccagggtga
     7261 cgtcctagat tgcgggcatg acgctcgcaa accgcttacg tgcccgcatg cgctggcgcg
     7321 gcattcgcag ccagacccag ctggcacgcc tgtccggcgt gtcgcaatcg tccatccacc
     7381 acatcctcac gcgcggcgac ggctatgccg ccaagcgcga gaccctgcgc cgcctggccg
     7441 cggccctggg caccacgccg gcctggctgg acggcgacac cggcgagccc cctgcgctac
     7501 gcgagccgca ccccgcctat gccgccgacg gctacgacgc cgagctccag gccctcatgg
     7561 cccgcctgcc cggccccgcc cggcgcaaga tcgtcgacat cgtccggctc atcgccgaga
     7621 acgccacgcc aaccgagcag acccaggagg aaaaacccgt tggcgacgcg gcgaccgatg
     7681 ataagcccga cccgtagcat gccgcgtagc cgggcagccc tacttctcct ggcccgccgg
     7741 ccgggaaacc accttgacct tgacctgcat ggccttgctg ccgcgcagca catccagggt
     7801 ggtttcccgc cctggcggca gcgaggcgat catggacagg aagcccgggg tattgagcac
     7861 gggcgtgccc gccaggcgct gcacgatgtc gcccaccttc agtccggccc ggcccgccgg
     7921 cccgtcatgc accacgctgg ccaggatcac gccgctcacg cccgtcggca gctggaaggc
     7981 ctgggccagg tcgggcgtga tgtcctgcgg ctctacgccc agccagccgc gctgcaccgc
     8041 gccgctgcgg atgatctcgt ccatgacccg gcgcgccgat tcgatgggaa tggcaaaacc
     8101 tatgcccagc gagccgccgg tctcggaata gatggccgta ttgatcccca ccagctgccc
     8161 gcgcgcatcg atcaaggcgc cgcccgaatt gccggggttg atggccgcgt cggtctggat
     8221 gaagttctcg aaggtgttga tgcccaggcc gttgcggccc agggccgaca cgatgccttg
     8281 cgtggtggtc agccccacgc cgaaggggtt gccgatggcc agcaccacgt cgcccacccg
     8341 caaggcatgc tcgggcgcca gcgccgccac cggcaggcgg cccagcccga ccgccagctt
     8401 gagcacggca agatcggttt ccggatccac gcccaccact ttcgcggcgg acttgcgccc
     8461 gtcggtcagc gccacctcga tggcatcggc ggcctccacc acgtggtagt tggtgagcac
     8521 atagccgtcc tcgcgcatga tcacgcccga gcccaggctg gaggccggct ggcgccggct
     8581 catgccaggc acctggccca gcaggtgctg cagcgtggga tcgtcgggca gcggcaccag
     8641 gggcacgtcg acatgcttgg tggtgtagat gttgagcacc cacggcaccg cgcgcgcggc
     8701 ggcatcggcg tacgacacca cgccgcgccc cgccgccggc ggcgcccggg gcgggaccgg
     8761 atcgccctga aggggcgccg gggtaggcac cggcacggcg cctgcctgcg gcggcatggg
     8821 gcgatagggc ccggccagcc gcagccagtc cggccgcaac gtggtgacga cgaataaaat
     8881 ggccagacag atcgtgacgg cctgcgcgaa tatcagccaa aggcgacgca tgatccccgg
     8941 gaacacctca atgatgaaaa cgatcgccac ccgcgaactg gccgcctggc tggacgacac
     9001 cctgcaggcc ccgcgcttca aggactattg tccgaacggc ctgcaggtcg aaggacgcga
     9061 gcgcgtaggc accattatcg ccggggtcac ggcctgcgag gcgctgttgc gcgccgccat
     9121 cgaacgcggg gccgacgccg tgctggtgca ccacggctgg ttctggaaga acgaagaccc
     9181 gcgggtacgc ggcccgcgcc gcacccggct ggccctggcc ctggcccacg atatcaatct
     9241 ctttgcctac cacctgccgc tggatgccca cccccggctg ggcaacaacg cccaattggc
     9301 ccgggtgctg ggcctggcgc ccgacaccga cgcagacggc cggccgcgga cctgcgggcc
     9361 cgacggcctg gtctggctgg gcaccgctcc cgggctggcc acgctgggcc ggctggccga
     9421 acgcgtcgcc cagcgcctga accgcacgcc cctggtcgtg ggcgacgcgg accagcccat
     9481 cggccgcgtg gcctggtgca ccggcggggc gcaaggcttc ctggacgccg ccatcgaggc
     9541 cggcgccgac gcctacctca ccggcgaagc ctccgaaccc accgtgcacc tggcccgcga
     9601 aaccggcacg ggctttctcg ccgccggcca ccacgccacc gagcgctacg gcgtgcaagc
     9661 cttgggttgc gccgtggcgg agcgcttcgg catcgaggtc gagttcgtcg atatcgacaa
     9721 tccggtctga cgcagcgcgc caggccacgc ccggcggctc gaaacggcca gtccagatct
     9781 tgaaatggct atagccaata tccagaactg gctatctgca ttaggaccaa tttcttctac
     9841 catggctggc gtcaccgctt acgtagggaa ggaacgacca tggtaggact cacccccgat
     9901 atcaagaccc gcatcctgca agccgtggac cagggctacg cggcgcagac cgacctgctc
     9961 gcccggctgg tgcgcattcc ctcgcaacgc ggcggggaag ccgaggcgca gcgcttcatg
    10021 gccggacaat acgcggcgcg cggctacgag gtggaccact ggcgcatcga cgtcgacgcc
    10081 atccgcgaca tgcccggctt ctcgcccgtg gccgtctcgt acgacgatgc ctacaacgtc
    10141 gtcggcacgc accggtcgcg cacccgggcg ggccgctcgc tcatcctcaa cggccacatc
    10201 gacgtggtgc ccgtgggccc cttggcgcaa tggagccgcg acccctacga tccggccatc
    10261 gtcgacggct ggatgcacgg ccggggcgcg ggagacatga agtccggcct gatcgcgtgc
    10321 atggcggcgc tcgacgcgct gcgcggcctg ggctgccaac cggcagccga tgtgttcctg
    10381 cagtcggtgg tcgaggaaga atgcacgggc aacggcgcgc tggcctgcct gcagcgcggc
    10441 taccgcgccg acgcggcctt catccccgag ccgttcacgc ccatgctgat gcgcgcccag
    10501 gtcggcccca tgtggttcca ggtgcaggtc gatggagatc cgcagcacgc ctccgcggcg
    10561 ttttccgagg cgggcgccaa tgccatcgag aaagccattt tcctgatcca ggccatgggc
    10621 gagctcgaag aaaaatggaa cggccgcaaa ggccagcacg cgcatttcca cgaccacccg
    10681 caccccatcc gcttcaacct cggcaagatc gccggcggcg actggccgtc cagcgtgccg
    10741 gcctggtgca ccttcgacat gcgcgtggcc gtgttcccgg ggcagagcct ggagaccgcg
    10801 cgcgccgaaa tcgaagcctt cgtggccgag gtcgcggcgc gcgacccctt ccttgccaag
    10861 catccgccca aggtggtcta tcatggcttc atggccgaag gctacgagct gaagaacgcg
    10921 gacgaggccg aggcagccct gcgcgccagc catgagcagg tattcaacga gccgctgcgg
    10981 gagcacgcca cgtcggcggc gaccgacgcg cgcttctacg gcctctacgc ggatacgccc
    11041 gccattgtct acgggcccga atgccgcatg ccgcacggct atgacgaggc ggtcaacctg
    11101 gaatcggtgc gcaaggtaac gcagaccatc gcccttttca tcgccgaatg gtgcgggctt
    11161 gaagagatcg ccgcatgacg acacgcagta acgccgccac gtccgagcag gccctgttcg
    11221 ccgcggatcc tcccaagctg tcgaccgccg aggcccgatc cctggccgag caggtctacg
    11281 gcttggcagc gcaagtgcag ctattgagca gcgagcggga ccagaacttc caactacgcc
    11341 tggacgacgg ccgcgccttc gtgctgaaag tcacgcaccc ggcggaagac ccggcggtga
    11401 ccgatttcca gacgcaagcc cagctgcatg tgatgcgcgc cgacggcgcg ctgcccgtgc
    11461 cgcacctgtt cccctcgcgc gacggcgcct acgtgcactg gcacgaaggg cccgacggca
    11521 cgcggcgggc ggtccgcctg atggccttcc tggagggcgt gccgctctac aaggcggcgc
    11581 gcagcgccgg gcaaggcgcg gcgatcgggc acgccctggc cagcttcgac cttgccctgg
    11641 ccgatttcga ccaccccatg gccggccacg agctgctctg ggacctccag cacgccgacc
    11701 gcctggcgga cctgctggcc cgcatcgggg aggccgaccg gcgcgcgctg gccgagcgcc
    11761 acatggagcg cttccggtcg cgcgtcaggc ccgccctggc cggcttgcgc cggcaggtca
    11821 tccacaacga cctcaacccc tacaacatga tggtgcaccc ccgcgacccg gcgcgcgtgg
    11881 cggccatcct ggatttcggc gacatggtcc gggccccgct ggcgcaggac ctggccgtgg
    11941 cctgcgccta tcgcctggaa gacgagggcg agctgctgtc ccaggccacg cccctggtcg
    12001 ccagctatca ccgggcgctg ccgctggccg aaacggagat cgcgctgctg cccgacctga
    12061 tcgcggcccg gctgctgatc acggtggcca tcaccggctg gcgcgccgag cagcacccgg
    12121 aaaaccgaac ctatatcctg cgcaacaacg gactggcctg gtcggggctg gcgcggctgg
    12181 acgcgctgcc gctgtcgcaa gtccaggacg tcctgttcaa cgcctgccgc accactctgg
    12241 agaatcccgc gtgagcaaag acaaagccat gctcaatgcc ttcgaccccg ccgccgcggg
    12301 ggagcttccc gccgccgagc gcgccatgat cgcgcgccgc cagcgcgtgc tgggacccgc
    12361 ctaccggctc ttctacgaca agcccctgca catcgtgcgc ggcgagggcg tgtggctgta
    12421 cggcgccgac gggcaacgct atctcgacgc ctacaacaac gtggcgtcgg tgggccacag
    12481 ccacccgcgc gtggtcgccg ccatcgccga gcaggccgcc gtgctgaaca cgcacacgcg
    12541 ctatctgcac gacggcgtgc tggactacgc cgagcgcctg ctggccacct tgcccgaacc
    12601 gctgtcccag gtcatcttca cctgcacggg cagcgaggcg aacgacctgg cgctgcgcgt
    12661 ggcgcgcagc tgcaccggcg ccagcggcgt gatcgtcacg cagctggcct accacggcgt
    12721 gacggcctcg gtcgccgccg tgtcgccctc gctgggcaag tccgtgccgc tgggcctgga
    12781 gacgcgcacc gtcgccgccc ccgacaccta ccgcctcgat ccggcgacgc tgggcgcgtg
    12841 gttcgccgcg caggtgcagt cggccatcga cgacatgctg cgccatggca tccggcccgc
    12901 cgccctgctg gtggacacca ttttctcgag cgacggcgtc tatgccgacc cggccggctt
    12961 cctggcgccc gcggtggagg cgatccgcgc ggccggcggc ctgttcatcg ccgacgaagt
    13021 gcaggccggc ttcgcgcgca ccggctcgcg catgtggggc ttccagcgcc acggcctggt
    13081 gccggacatc gtcaccatgg gcaagcccat gggcaacggc catcccatcg cggccatggt
    13141 ggcgcgcccc gatgtgctcg agcgcttcgg ccgcgagtcg cgctacttca acacgttcgg
    13201 cggcaacccg gtgtcgtgcg cggccgccgc ggcgaccctg agcgtgatcc aggacgaagg
    13261 cctgcaggcc aacgcggcgc gcaccggcca gtacctgcgc gagggctttc gccagctcgc
    13321 gcagcgccac gagcgcatcg gcgatgtccg gggagacggc ctgttcatcg gcgtcgagct
    13381 ggtgcaagac cgcgcctcca aggcacccgc caaagaagcc atgcacggct tcgtcaatcg
    13441 catgcgcgaa gagcgcatcc tgctgagcgc caccggcctg cacggcaacg tgctcaagct
    13501 gcgcccgcag ctgccgttcg gccgcgagca cgccgacatc ctgctcgcag ccgcggacaa
    13561 ggtgctggcc gggctttgag cgccgcggcg cgcggccagc gcgcccggtc gcgctggccg
    13621 ccttaccgct tgctttccgc cacccgggcc aaggcccggg ccaggcggtc gacgccgtcg
    13681 gcgatgtgcg cggtgggcac gccgccatac cccagcacga ccgcgttgcg gtcccggccg
    13741 tcatggtcgg gcaggaagtg gggcatgccc agcaccaggc cttcggcccg ggccgcctcg
    13801 gccagccgcg ccgccggcac gcgcggcgcc agccgcgcat agacatgcag ccccgccctg
    13861 gcctcggaca gcgtcatcag gtccccggcc ttggcgtgca gcgcctcgcg cagcacgccc
    13921 tggcgctcgg cgtattcgac gcgcatgcgc ttgatgttgt gcgagaaatg gccgtccgcc
    13981 atgaaatccg ccagggcggc ctgcaagtgc aggtggccgg gccggtagat gcgcgcgcag
    14041 gcattcgaga acgcgtgcgc cacgtgcccg ggcaccacca tatagctcat gcgcaggccg
    14101 gcgaacaggg tcttgctgaa ggttcccatg tagatgacgc ggccgtccgt atccagccct
    14161 tgcaaggaag ggatgggcgc gccgtcgtag cggaattcgc tgtcgtagtc gtcctcgatg
    14221 atccagagcg cccggtcgcg cgccgcctgg atcagttcga ggcggcgcga cagcggcatg
    14281 acgacgccgg tcgggtactg gtgcgaaggg ctggtgatta gcatgcgcgt gcgccggggg
    14341 atgcggcccg gcgccagggt gacgccatgc tcgtcgagcc cggccgcccg cacgcccagg
    14401 ccggcggcgg cgagcaccgg gggaaaagcc cagtggcatg ggctttccac cagcgcggcg
    14461 tcgcccgcgt cggccagcag gcgggcgcac aggtccaccg attgatgcgt gcccgccgtg
    14521 atgatcacct gcgagggctc gcagacgacc gagcgcgaga tgcgcaggta gttcgcgatg
    14581 gtttcccgca acggcaggaa gcccgcgccg tccttggcgt agccggcgag cgcaaggttg
    14641 gacttgtcga ggtggcgcgc cagcaggcgc cgccagatgt ggaagggaaa cagcgcggcg
    14701 tcggcgatgc ccggcacgaa agcgccgctg tgctgcccaa ggccgccggt gccgctgccg
    14761 atgactgcgc cgcgcgtcga gatcccgggc cgctggcgcg cgatctcggc ctgcgagatg
    14821 ccggccagcg gccgctcgac ggtccgctcg acgaaggtgc cgctgccgct gcgcgcggtg
    14881 agatagcttt cggcgatgag ccggtcgtag gccagcgata ccgtgatgcg cgagatgccc
    14941 agctcatcgg ccagcgcgcg cgaggaaggc agccgctggc ccgccaccag cgtgtggtcg
    15001 agcacgcccc gccgcacgat ctcgtatatc tgcctttgca gcgactgttc cgcctcgcgc
    15061 gacagcgcgc ccgcgagcaa atcggacagc aggacttctt tcatttttaa ttggacttac
    15121 aacatctgtt tttgtggcta tataaggtat agccagtggc tgctatcgtc aacgccgttg
    15181 ttcaaaacca atcaagggga aatgcatgaa cggcaatacg agccggccgc a
//
LOCUS       DASWPO010000215        10593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1479538, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000215 DASWPO010000000
VERSION     DASWPO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479538"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2840)
                     /locus_tag="VGC69_11475"
     CDS             complement(<1..2840)
                     /locus_tag="VGC69_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0295940.1"
                     /translation="MNEQERFNLSAWTLRHKSLVFFLMGMIALFGMLSYKNLAQSEDP
                     PFTFKVMVIQTLWPGASAKQVQEEVTDRIARKLQEIPSTDFLRSYSRPGESLVFFNLK
                     DSAPASVVPGTWYQVRKKIGDIAAQLPQGVQGPFFNDEFGDVYTNIYALQGDGYTPAQ
                     RHDYADQLRTELLRVPGVAKVDYFGDQNQHVYIDIDNAQLAKLGITAQQIGQAINAQN
                     AVASAGVMTTADDRVFVRPTGQLADTGTLADTLLHINGRSIRLGDIAVIRRGYDDPPG
                     QLMRFMGKPSLGIGITMQPGGDVIKLGTALDQATQALQRRLPAGLELAQVTSMPSAVS
                     QSVDDFLESVAEAIAIVLVVSLLSLGLRTGMVVVISIPFVLAATAFFMDVFGIGLHKV
                     SLGTLILALGLLVDDAIIAVEMMSVKLEQGWNRLRAAAFAYTSTAFPMLTGTLVTVSG
                     FLPIALAKSGTGEYTRSIFEVSAIALLVSWLAAVIAIPLLGYYLLPEHHQRARPGQEW
                     WARLLPRRLREARLARAAQQPAHEGGEIEIYDSPFYRRLRGWLGFCLRRRWLVLGSTV
                     ALFVVALAGFGLVPKQFFPSSDRPELLVDLRLAEGASIQATQREAQRLEKALQDRPEI
                     ANYTSFVGAGAPRFYLPLDQQLAQPNFAQFLVTAKDIKQREQLAAYLEGMLKRDFGNA
                     RTRVSRLENGPPVGFPVQFRVSGDDIATVRGIAEQVAAAMREDPDTRGVQFDWDEPSE
                     RSVRFEVDQIKARQLGISSNDIAGFLAMTLSGTTVTQLRERDKLISVDLRGAPGDRVH
                     PDQIERLAMPTADGRAIPLAQLGHAVYGLEYGVVWERDRQPSITVQSDIRSGTQGLDV
                     THAIDKRLDAIRAHLPVGYLVEVGGSVEQNTKAQDSINVEMPIMVVAVLTFLMIQLQS
                     FGRMVMVVLTAPLGLIGVIGALLLFHQPFGFVA"
     gene            complement(2837..3997)
                     /locus_tag="VGC69_11480"
     CDS             complement(2837..3997)
                     /locus_tag="VGC69_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0295941.1"
                     /translation="MSHRSSFFLPFSGRLEPGRAARRALLPGAAVLALALLAGCGDKT
                     SNSEAAVPVIAQPVQAAGGVEASRFPGAVHARFEMPLSFRVAGQLTARYVEAGAAVKK
                     GQPLAQLDPADAGSQQAAARAALDAAEHRLLYAAQQRGRDAAQARQNLISQLQLEQTQ
                     DAYAAALAARDQARQQYQLAQNQSRYTTLTADHDGVIAGLRAEVGEVLTAGQAVYDYA
                     WDGEREVRIDVPESRIGGLALGQAATVRLPARPGREYAARVREIAAAADPQSRTYLVR
                     LTLDPAGGDLPPLGMTADVALQGTAAAPGQVRIPATALFHQGEQPAVWVVGADNTLAL
                     RPVSVRRYGERDVLVASGLQAGERIVMQGVHTVSAGMKVELVNPPHPEDAPR"
     gene            4130..4747
                     /locus_tag="VGC69_11485"
     CDS             4130..4747
                     /locus_tag="VGC69_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0295942.1"
                     /translation="MSRQRLTREESREQTRRRLLDSAAALLAKKGLAATSVEDIAKHA
                     GYTRGAFYSNFKSKVDLFIDLLKCSHQHIQQDLQGLLDADIQGEDLRQQLVQFYARCH
                     GDAPSYVLWAEARLYAARDAKFRQRLNQLYLEKREVIAYFITQFCQKMGTAPPAPAED
                     LALGLIALADGVFYFNLSMPALLPDGAAQSLLSVFFTSTFFGARA"
     gene            4888..6183
                     /locus_tag="VGC69_11490"
     CDS             4888..6183
                     /locus_tag="VGC69_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY0295943.1"
                     /translation="MGKLILAISGNNLAASQAPQRLARLRAMTGSGLLVAVGYIDPGN
                     WATDIAGGSHYGYGLLAVVLVSALLAMGFQVLTARLALATGLDLAVLTTRYLPRPLAR
                     AAWLAGEAAILATALAELIGGAIALRLLFGLPLMGGVGITALGTLAVFLYTQGQNERH
                     ELVIGALLGVVALSFLYLLARAQPSAAEMLQGVAATGSTLRHADAFLIALGILGATLM
                     PHNLYLHSGDLAQRAAGLDQADRPLAMRIARNDTLAALGLAMLINAAILIVAASSLSG
                     RNGPAVDSLDGAHAALGATLGAAAAAIFAIALYAAGQSSTITGVMAGGILSRGFRQGR
                     QSSDRRRAFLTRAVAGAAALGLLAATGGQSPDELLVLSQVVLSLALPFALVPLVVLAC
                     RRDVMGRYAIQGIGRAAAVLATACIVVLDLYLLWDTLAG"
     gene            complement(6272..6487)
                     /locus_tag="VGC69_11495"
     CDS             complement(6272..6487)
                     /locus_tag="VGC69_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295944.1"
                     /translation="MLRQENTMQDAIHTLAQRRQAVLKPLRACWLRLQDLVDETVRML
                     ADGMSSQATRRMRPIPIRIDERRDPRR"
     gene            complement(6670..8382)
                     /gene="ptsP"
                     /locus_tag="VGC69_11500"
     CDS             complement(6670..8382)
                     /gene="ptsP"
                     /locus_tag="VGC69_11500"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY0295945.1"
                     /translation="MRGKGVARGYAIGRAIIMGAAALEVAHYRIAPEDVESECRRLKD
                     ALARAQADLLHLANTLPADAPRELGAMLNVHSLLLGDPLLVEQAQALIHGRHYNAEWA
                     LTAQGQLLGEQFDAMEDEYLRERGADVRQVIERVLHVLSGTPALLPEEKVCSGGNEPL
                     VVVARDISPADMLRLRGARFSAFVTDLGGPTSHTAIVARSMGVPAVVALGNIRDLVRD
                     GDMLIVDGASGTVLVNPSQSMLAQYRQLQRIYADERAELELLRDEPSVTLDGINVALE
                     ANIELPEEAAVALAAGAQGIGLFRSEFLFMGRAELPGEEEQYQAYASVVKVMAGRPVT
                     IRTLDIGSDKTLDDEVTVATNPALGQRAIRYCLARPEMFATQLRAILRASAHGPVRIL
                     IPMISHMHEVEATFAAIESARRELDARGQAYAPKIPVGAMVEVPAIAIAIEPFVQELD
                     FLSIGTNDLIQYVLAIDRGDHDVASLYDPLHPAVLRLLAHTINAGERAGKPVAVCGEM
                     AGDSRYTRLLLGLGLSEFSMHPQQLLDVKREVRRAHSNALRARVANVLNRALPVDLAR
                     LEQP"
     gene            complement(8435..8704)
                     /locus_tag="VGC69_11505"
     CDS             complement(8435..8704)
                     /locus_tag="VGC69_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY0295946.1"
                     /translation="MATSDIVISNKLGLHARAAAKLTQLASRFSSEIFIARGAQRVNA
                     KSIMGVMMLAAGMGTTVRLEATGGDADLALSEIQNLFDNKFGEHE"
     gene            complement(8745..9146)
                     /locus_tag="VGC69_11510"
     CDS             complement(8745..9146)
                     /locus_tag="VGC69_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEY0295947.1"
                     /translation="MTGIVIVVHAPLGEAMHEMARHVLGDGIADTLAVHDIQPDDQPE
                     DQAPAVLDDIRRLGRGGGVLVLTDLVGATPANIAKRAVAQAQAEGLNCCALAGLNTPM
                     LLRALTYRNLSLAETREKALAGGVQGCLQVD"
     gene            complement(9239..10195)
                     /gene="gshB"
                     /locus_tag="VGC69_11515"
     CDS             complement(9239..10195)
                     /gene="gshB"
                     /locus_tag="VGC69_11515"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY0295948.1"
                     /translation="MHVLFVIDPLPLLKAYKDSSVAMMRALVARGHTLSVALQGELFI
                     DMGVVRARTTPIALVAGADLHEHAWWRETGAASEVPLSSFGAVVMRKDPPFDMEYVYS
                     THLLGYAQAQGARVFNRGPAILSHPEKLAITEFPELTAPTLVTRDMARIKAFHALHHD
                     VIVKPLDGMGGTGIFRLRAEEPNRNAILETLTLNGTQTIMAQRYIPEIAKGDKRVLLI
                     GGEPVPYCLARIPQAGETRGNLAAGGRGVAQPISERDRFIAETVAKRIAGRGLLLVGL
                     DVIGDCLTEVNVTSPTCFVEITEQTGFDVGDCFAQALERACA"
     gene            complement(10333..>10593)
                     /gene="infC"
                     /locus_tag="VGC69_11520"
     CDS             complement(10333..>10593)
                     /gene="infC"
                     /locus_tag="VGC69_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY0295949.1"
                     /translation="TDEGDYQVKLRNLKRFLEEGDKAKVTLRFRGREMAHQELGMRVL
                     ERVRDDLTELALVEAMPKLEGRQMVMVLAPKKKAAGKTDVPA"
BASE COUNT         1676 a         3661 c         3546 g         1710 t
ORIGIN      
        1 gccacgaagc cgaagggctg gtgaaacagc agcagcgcgc cgatcacgcc gatcaggccc
       61 agcggcgcgg tcagcaccac cattaccatc cgcccgaagc tttgcagctg gatcatcagg
      121 aaagtcagca cggccacgac catgatcggc atctccacat tgatcgaatc ctgggccttg
      181 gtgttctgct cgaccgagcc gccgacctcc acgaggtagc ccaccggcag atgggcgcgg
      241 atggcgtcga gccgcttgtc gatggcatgg gtcacgtcca gcccctgggt gcccgagcgg
      301 atgtcggact gcaccgtgat cgacggctgg cggtcgcgct cccagacgac cccgtactcc
      361 aggccgtaca cggcatgccc gagctgggcc aggggtatcg cccggccgtc ggccgtcggc
      421 atcgccagcc gctcgatctg gtcgggatgg acgcgatcgc ccggcgcccc gcgcaggtcg
      481 accgagatca gcttgtcgcg ctcgcgcaac tgcgtcaccg tcgtgcccga cagcgtcatg
      541 gccaggaagc cggcgatgtc gttcgagctg atgccgagct ggcgcgcctt gatctggtcg
      601 acctcgaaac gcaccgaacg ctcggacggc tcgtcccagt cgaactgcac gccgcgcgtg
      661 tcgggatcct cgcgcatggc cgcggcgacc tgctcggcga tgccccgcac cgtggcgata
      721 tcgtcgcccg agacgcggaa ctgcaccgga aagcccaccg gcggcccgtt ctccaagcgg
      781 ctgacgcggg tgcgcgcatt gccgaaatcg cgcttgagca tgccctccag ataggccgcc
      841 agctgctcgc gctgcttgat gtccttggcg gtgacgagga actgcgcgaa attgggctgc
      901 gccagctgct ggtccagcgg caggtagaag cgcggcgcgc cggcccccac gaagctggtg
      961 tagttggcga tctccggacg gtcctggagc gccttctcca gacgctgcgc ctcgcgctgc
     1021 gtggcctgga tcgaggcgcc ttcggccagg cgcaggtcga ccagcagctc ggggcggtcc
     1081 gaactgggaa agaactgctt gggcaccagg ccgaaaccgg ccagcgccac cacgaacagc
     1141 gccacggtcg accccagcac cagccagcgc cggcgcaggc agaaacccag ccagccgcgc
     1201 aggcgccggt agaacgggct gtcgtagatc tcgatctcgc cgccctcgtg cgcgggctgc
     1261 tgcgcggccc tggccaggcg cgcctcgcgc aggcgccgcg gcagcagccg ggcccaccac
     1321 tcctgcccgg ggcgggcgcg ctggtgatgt tcgggcagca ggtagtagcc cagcagcggg
     1381 atcgcgatca ccgccgccag ccacgacacc agcaaggcga tcgccgacac ctcgaagatc
     1441 gaacgcgtgt attcgccggt gcccgacttg gccagggcga tgggcagaaa gcccgacacc
     1501 gtcaccagcg tgccggtcag catcggaaag gccgtgctgg tataggcgaa tgcggccgcg
     1561 cgcaggcggt tccagccctg ctccagcttc accgacatca tctccacggc gatgatcgcg
     1621 tcgtcgacca gcaggcccag cgccaggatc agcgtgccca gcgaaacctt atgcagcccg
     1681 atgccgaaca cgtccatgaa gaaggccgtg gccgccagca cgaaggggat ggagatgacc
     1741 accaccatgc cggtgcgcag ccccaggctc agcaggctga ccaccagcac gatggcgatg
     1801 gcctcggcga ccgactccag gaagtcgtcg acggattgcg acaccgcgct cggcatgctg
     1861 gtgacctggg ccagttcgag gcccgcgggc aggcgccgct gcagcgcctg cgtggcctgg
     1921 tccagggccg tgccgagctt gatcacgtcg ccgccgggct gcatggtgat gccgatgccc
     1981 agcgacggct tgcccatgaa acgcatcagc tgcccgggcg gatcatcgta gccgcgccgg
     2041 atgacggcga tgtcgcccag ccggatgctg cggccgttga tgtgcagcag cgtatcggcc
     2101 agcgtgccgg tgtcggccag ttgcccggtg ggccgcacga acacgcggtc gtcggccgtg
     2161 gtcatcacgc cagccgaggc caccgcgttc tgcgcgttga tcgcctggcc gatctgctgc
     2221 gcggtgatgc ccagcttggc caattgcgcg ttgtcgatgt cgatgtagac gtgctggttc
     2281 tggtcgccga aataatcgac cttggccacg cccggcacgc gcagcagctc ggtgcgcagc
     2341 tggtcggcgt agtcgtgccg ctgcgccggg gtgtagccgt cgccctgcag ggcatagata
     2401 ttggtgtaga cgtcgccgaa ctcgtcgttg aagaacggcc cctgcacgcc ctgcggcaac
     2461 tgcgcggcga tgtcgccgat cttcttgcgc acctggtacc aggtgccggg cacgacggac
     2521 gcgggcgccg aatccttgag gttgaagaac accagcgact cgcccgggcg ggaatagctg
     2581 cgcaggaaat cggtggatgg gatctcctgc aatttgcggg cgatgcggtc ggtgacctct
     2641 tcctgcacct gcttggcgct ggcgccgggc cagagggtct ggatcaccat gaccttgaaa
     2701 gtgaaaggcg gatcttccga ctgcgccagg ttcttgtacg acagcatgcc gaacaaggcg
     2761 atcatgccca tcaggaagaa caccagcgac ttgtgccgca aggtccaggc ggaaagattg
     2821 aaacgctcct gctcgttcat cgcggcgcgt cctcgggatg cggcgggttc accagctcga
     2881 ccttcatgcc cgccgagacc gtatgcacgc cctgcatgac gatgcgctcg ccggcctgca
     2941 gaccgctggc caccagcacg tcgcgctcgc catagcgccg taccgagact ggccgcaatg
     3001 ccagcgtgtt gtcggcgccc accacccaca ccgccggctg ctcgccctgg tggaacaggg
     3061 ccgtggccgg gatgcgcacc tgccccggcg cggcggccgt cccttgcagg gcgacgtcgg
     3121 ccgtcatgcc cagcggcggc agatcgccgc ctgccggatc gagcgtcagc cgcaccaggt
     3181 aggtccgcga ctgcgggtcg gccgcggccg cgatctcgcg cacccgggcg gcgtattcgc
     3241 gcccggggcg ggccggcagg cgcaccgtcg cggcctggcc cagggccagg ccgccgatgc
     3301 ggctttcggg cacgtcgatg cggacttcgc gctcgccgtc ccaggcatag tcatagaccg
     3361 cctggccggc ggtcagcacc tcgcccacct cggcgcgcag gcccgcgatg acgccgtcgt
     3421 ggtcggccgt gagcgtggtg tagcgcgact ggttctgcgc cagctggtat tgctggcggg
     3481 cctggtcgcg cgccgccagc gcggcggcat aggcatcctg cgtctgctcc agctgcagct
     3541 ggctgatgag gttctgccgg gcctgcgccg cgtcgcggcc gcgctgctgg gcggcgtaca
     3601 gcaggcggtg ctcggccgcg tccagcgccg cgcgggcggc ggcctgctgg ctgccggcgt
     3661 cggccgggtc gagctgcgcc aggggctggc ccttcttcac cgcggccccc gcctcgacgt
     3721 agcgcgccgt caactggccg gccacccgga acgacaaggg catctcgaaa cgcgcgtgca
     3781 cggcgccagg gaagcggctg gcctcgacgc cgccggccgc ctgtaccggc tgggcgatga
     3841 cgggcaccgc tgcctcggag ttggacgtct tgtcgccgca gccggccagg agcgccaggg
     3901 ccaggacggc cgcgccgggc agcaaggcgc gccgcgccgc cctgcccggc tcaagccggc
     3961 cggaaaacgg aagaaaaaag gatgaacgat ggctcactgg ccgctcctga acgcggcagc
     4021 cggaccggct tgccgtcttt tggatacgag aaagaattct aatacagtat tgaatctgaa
     4081 ttcaaatatg tatttaatat tggcatcatg ccccgtgata cgatcaaaca tgtcccgcca
     4141 acgcctgacc cgtgaagaaa gccgcgaaca aacgcgtcgg cgcctgctgg actccgccgc
     4201 ggcgctgctt gccaaaaagg gcctggccgc gaccagcgtg gaagacatcg ccaagcatgc
     4261 cggctacacc cggggcgcct tctattccaa cttcaagagc aaggtcgacc tgttcatcga
     4321 cctgctcaaa tgcagccacc agcacatcca gcaagacctg caaggcctgc tcgacgccga
     4381 catccagggc gaggacctgc gccagcagct ggtccagttc tacgcccgct gccacggcga
     4441 tgcccccagc tacgtgctgt gggccgaggc gcggctgtac gcggcgcgcg atgccaaatt
     4501 ccgccagcgc ctgaaccagc tctacctcga aaagcgcgaa gtcatcgcct atttcatcac
     4561 ccagttctgc caaaaaatgg gcacggcgcc gcccgcgccg gccgaggacc tcgccctggg
     4621 cctgatcgcg ctggccgacg gcgtgttcta tttcaatctc agcatgcctg ccctcctgcc
     4681 ggacggcgcc gcgcaatccc tgctgagcgt atttttcacc tcgaccttct tcggggcccg
     4741 cgcctgaccc agccgcgccg tctgcatctc cagccggccg gccgcgcttc caaataggtt
     4801 ttctctagaa atcccattgt ttctattgat tggacctatt taaaatctta cttgacaatg
     4861 agacacattc tcaataaaag taaggccatg ggcaagctga tcctcgcgat ttccggcaac
     4921 aacctcgccg ccagccaggc gccgcagcgc ctggcgcggc tgcgcgccat gacgggctcg
     4981 ggcttgctgg tggccgtggg ctatatcgat ccgggcaact gggccaccga catcgccggc
     5041 ggcagccact atggctacgg cctgctggcc gtggtgctgg tctccgccct gctggccatg
     5101 ggttttcagg tgctgaccgc ccgcctggcc ctggccaccg gcctggacct ggccgtcctc
     5161 accacccgct acctgccccg cccgctggcg cgcgccgcct ggctggccgg cgaagccgcc
     5221 atcctggcca ccgcattggc cgaactgatc ggcggcgcca tcgcgctgcg cctgctcttc
     5281 ggcctgccgc tgatgggcgg ggtcggcatt accgcgctgg gcaccctggc cgtgtttctc
     5341 tacacccagg ggcaaaacga acgccacgag ctggtcatcg gcgcgctgct gggcgtggtg
     5401 gcgctgtcct ttttgtacct gctggcgcgc gcccagccct cggccgccga aatgctgcaa
     5461 ggcgtggccg ccaccggcag caccctgcgc catgccgacg ccttcctgat cgcgctgggc
     5521 atcctgggcg cgacgctcat gccgcacaat ctttatctgc actccggcga cctggcgcag
     5581 cgcgccgccg gcctggacca ggccgaccgc ccgctggcca tgcgcatcgc ccgcaacgac
     5641 acgctcgccg ccctgggcct ggccatgctg atcaacgccg ccatcctgat cgtggccgcc
     5701 agctcgttgt cgggccgcaa cggcccggcc gtggacagcc tggacggcgc gcatgccgcc
     5761 ctgggcgcca cgctgggcgc ggccgccgcg gccatcttcg ccatcgcgtt gtacgcggcc
     5821 ggccaaagct ccaccatcac cggcgtcatg gccgggggaa ttctttcgcg gggcttccgg
     5881 cagggccggc aaagctccga ccgccgccgc gccttcctca cgcgcgccgt cgcgggtgcc
     5941 gcggccctgg ggctgctggc cgcgaccggc ggccaaagtc cggacgaact gctggtgctc
     6001 agccaggtcg tgctcagcct ggccttgccg ttcgcgctgg tcccgctcgt cgtgctggcc
     6061 tgccggcgcg acgtgatggg ccgctacgcc atccagggca tcgggcgcgc cgccgccgtc
     6121 ctggccacgg cctgcatcgt ggtgctggac ctttacctgc tatgggacac gctggccggg
     6181 taaagacccg ggatgagccg tgaaaagcca tgaaaaaagc cggccaggca tggggcctgg
     6241 ccggctttca tttttgaaac ctgggaagga atcagcggcg cggatcgcgg cgttcgtcga
     6301 tgcggatcgg gatgggacgc atgcggcgcg tggcctggct cgacatccca tcagccagca
     6361 tccggacggt ctcgtcgacc agatcttgga gtcgcaacca gcaggcgcgc agcggcttga
     6421 gcacggcttg gcgacgctgg gcaagggtgt ggatggcgtc ctgcatggtg ttctcctgac
     6481 gaagcattcg acttcatctt agacctgtcg attttgcgtc gcaataaata tttgacaatc
     6541 gtgtgatgaa aagcggcatt ccgggctgtt ttggcgcccg ttatcgccct gtcctgtcac
     6601 aaacaggcaa acaaacccgg ggagaaatcc gggccgcatc atcaacccga ccaggccagc
     6661 cttgacggat cagggctgct ccagcctggc caggtcgacc ggcaaggcgc ggttgagcac
     6721 attggccacc cgcgcgcgca aggcgttgga atgcgcgcgg cgcacctcgc gcttgacgtc
     6781 cagcagctgc tgcgggtgca tggagaattc ggacaggccc aggcccagca gcaggcgtgt
     6841 gtagcgcgaa tcccccgcca tttcgccgca cacggccacc ggcttgccgg cgcgctcgcc
     6901 cgcgttgatg gtgtgcgcca ggaggcgcag caccgccggg tgcagcggat cgtacaggct
     6961 ggccacgtcg tggtcgcccc ggtcgatggc cagcacgtac tggatgaggt cgttggtgcc
     7021 gatggacagg aagtccagct cctgcacgaa gggttcgatg gcgatggcaa tcgccggcac
     7081 ctcgaccatg gcgcccacgg gaatcttggg cgcataggcc tggccgcgcg cgtcgagctc
     7141 gcgccgcgcc gactcgatgg cggcgaaggt ggcttcgacc tcgtgcatgt gcgagatcat
     7201 gggaatgagg atgcgcaccg gcccgtgcgc cgaggcgcgc aggatggcgc gcaactgggt
     7261 ggcgaacatc tcgggccggg ccaggcaata gcggatggcc cgctgcccca gcgccggatt
     7321 ggtggccacc gtgacctcgt cgtccagcgt cttgtcggag ccgatatcga gcgtgcggat
     7381 ggtgaccggc cgccccgcca tgaccttcac caccgaggca taggcctggt attgctcttc
     7441 ctcgcccggc agctcggcgc gccccatgaa gaggaattcg ctgcggaaca ggccgatgcc
     7501 ctgcgcgccg gcggccagcg cgacggcggc ctcctcgggc aattcgatat tggcttccag
     7561 cgccacattg atgccgtcga gcgtgaccga gggctcgtcg cgcagcagct ccagctcggc
     7621 gcgctcgtcg gcgtagatgc gctgcagttg gcggtactgc gccagcatgc tctgcgaagg
     7681 attgaccagc accgtgcccg aggcgccgtc gacgatgagc atgtcgccgt cgcgcaccag
     7741 gtcgcggata ttgcccaggg ccaccacggc cggcacgccc atgctgcggg ccacgatggc
     7801 cgtatgcgac gtggggccgc ccaggtcggt gacgaaggcg gagaagcgcg cgccgcgcag
     7861 gcgcagcatg tccgccgggg aaatgtcgcg cgccaccacc accaggggct cgttgccgcc
     7921 gctgcagacc ttctcttcgg gcaacagcgc cggcgtgccc gacagcacgt gcagcacccg
     7981 ctcgatcacc tggcgcacgt cggcgccgcg ctcgcgcagg tattcgtctt ccatggcgtc
     8041 gaactgctcg cccagcagct ggccttgggc cgtcagggcc cattcggcgt tgtagtggcg
     8101 tccgtggatc agcgcctggg cctgctcgac caggagcggg tcgcccagca gcaggctgtg
     8161 cacattgagc atggcgccga gctcgcgcgg cgcgtcggcc ggcagcgtat tggccaggtg
     8221 cagcaggtcg gcctgggcgc gcgccagcgc gtccttgagc cgtcggcatt cgctttcgac
     8281 gtcttcgggc gcgatgcggt agtgcgccac ctcgagggcg gcggcgccca tgatgatcgc
     8341 tcggccaatg gcatagccgc gcgccacgcc cttgccgcgc agcgccagga ctgcgctggg
     8401 cggctcggcc agcgccacgc cgccggatgg gtcgctattc gtgctcaccg aacttgttgt
     8461 cgaacagatt ctggatttca gacagggcca ggtcggcatc gccgcccgtg gcctccaggc
     8521 gcaccgtggt gcccatgccc gcggccagca tcatcacgcc catgatgctt ttcgcattga
     8581 cccgctgcgc gccgcgcgcg atgaagatct cgctgctgaa ccggctggcc agctgcgtga
     8641 gcttggccgc cgcgcgtgca tgcaatccga gcttgttgct gatgacgatg tctgaagtag
     8701 ccatagagtg atgagccaaa aacaatgcgc ttaaaacaat gcgactagtc tacctgcagg
     8761 cagccttgca cgcctccggc cagggctttt tcgcgcgttt cagccaatga caaattgcga
     8821 taagtcagcg cccgcagcag catgggggtg ttcaggcccg ccagtgcgca gcagttcaac
     8881 ccctcggcct gcgcctgggc caccgcgcgc ttggcgatat tggccggcgt ggcgccgacc
     8941 agatcggtca gcaccagcac gccgccgccg cggccaaggc gcctgatgtc gtccaatacc
     9001 gcgggcgcct ggtcctcggg ctggtcgtcg ggctggatgt cgtgcacggc cagcgtgtcg
     9061 gcgatgccgt cgcccagcac gtgccgggcc atctcatgca tggcctcgcc cagcggggcg
     9121 tgaacgacga tgacgatgcc cgtcatatcc gctccgccgg gccgccccta ggagcggata
     9181 cgccccttcg ggaggcagcg aacgcagtga gcgtgggggt cgtcatatcc ggttcctatc
     9241 aggcgcaggc gcgctcgagc gcctgcgcaa agcaatcacc gacatcgaag cccgtctgtt
     9301 cggtgatctc gacgaagcag gtcgggctgg tgacgttgac ctcggtgagg cagtcgccga
     9361 taacgtccaa tcccaccagc agcaagccgc ggccggcgat gcgcttggcc accgtctcgg
     9421 cgatgaaacg gtcgcgctcg gaaatcggct gggccacgcc gcggccgccc gcggccaggt
     9481 tgccgcgcgt ttcgccggcc tgcggaatgc gcgccaggca atacggcacg ggctcgccgc
     9541 cgatcagcag cacgcgcttg tcgcccttgg cgatctcggg gatgtagcgc tgcgccatga
     9601 tggtctgcgt gccgttcagg gtcagcgtct cgaggatggc attgcgattg ggctcctcgg
     9661 cgcgcaagcg gaagatgccc gtgccgccca tgccgtcgag cggcttgacg atgacgtcgt
     9721 ggtgcagcgc atgaaaggcc ttgatgcgcg ccatgtcgcg cgtgaccagc gtgggcgcgg
     9781 tgagctcggg gaattcggtg atggccagct tttcgggatg gctgaggatg gccgggccgc
     9841 ggttgaacac gcgggcgccc tgtgcctgcg catagccgag caggtgcgtg gaatacacgt
     9901 attccatgtc gaagggcggg tccttgcgca tgaccacggc gccgaaactg gacaggggca
     9961 cctcggaagc cgcgccggtc tcgcgccacc aggcatgctc gtgcaggtcc gcgcccgcca
    10021 ccagggcgat gggggtggtg cgcgcgcgca ccacccccat gtcgatgaag agctcgccct
    10081 gcaaggccac gctgagcgta tggccgcgcg cgaccagcgc ccgcatcatg gcgacggaac
    10141 tgtccttgta cgccttcagc agcggcaagg gatcgatcac gaacaaaaca tgcatcggtt
    10201 tcataccccg ggtcctatac ccgattcatg gtaactgaat tctgcctagc ctaagcgcat
    10261 acccctacaa ggccaacggc ccggccgccc cgctgtcggg atggccgggc catgcacatt
    10321 gccgttgccg gatcaggccg ggacgtcggt cttgccggcg gcctttttct tgggcgccag
    10381 caccatgacc atctggcggc cttcgagctt gggcatggct tcgaccaggg ccagctcggt
    10441 gaggtcgtcg cgcacgcgtt ccagcacgcg catgcccagc tcctggtggg ccatttcgcg
    10501 gccgcgaaag cgcagcgtga ccttggcctt gtcgccttct tccaggaagc gcttcagatt
    10561 gcgcagcttg acctggtaat cgccctcgtc ggt
//
LOCUS       DASWPO010000216        19715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1300105, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000216 DASWPO010000000
VERSION     DASWPO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 19715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19715
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300105"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VGC69_11525"
     CDS             <1..697
                     /locus_tag="VGC69_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0295950.1"
                     /translation="GCHARAVQPVADLLPRGARGLRIGVLGGYFAQYAGPAASEAVRL
                     AAGALGAVATVDWPDAALGRAAAFIVTAGEGGSLHLPDLRTRAADFEPLSVDRFMAGA
                     LQPAHWVTRAQRFRRVYRDRVNALFADWDVLLAPATPVPAPAIGAEWIDINGERHLCR
                     PSMGLLTQPVSFAGCPVVAAPLWPAAGQGLPLGVQLIAAPWREDLALRAAHALEQAGI
                     AGLRPAFFASTTL"
     gene            697..1071
                     /gene="hpxZ"
                     /locus_tag="VGC69_11530"
     CDS             697..1071
                     /gene="hpxZ"
                     /locus_tag="VGC69_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HEY0295951.1"
                     /translation="MDINLPDVLAEVQRQFDRYEKALTGNDVAVLDELFWDSPHTLRY
                     GATENLYGYAQIRDFRAARPSQGLAREVLKTVITTYGRDFATCNIEFRRAGVPGTGRQ
                     SQAWVRTPAGWRVVAAHVSMLR"
     gene            1121..1849
                     /locus_tag="VGC69_11535"
     CDS             1121..1849
                     /locus_tag="VGC69_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0295952.1"
                     /translation="MQPIPPPQPKAAIVHRTRADDVYDLLKRDIAEFHLVPGDRFTEG
                     EISERLGVSRTPVRQALFRLQQEGYVEVLFRSGWRVLPFDFKLFEQLYDFRLVLETEA
                     VRRLCTMGAEAARQALAGLEAVWLVPPDARSTDMAQVGRWDEAFHCSLLDASGNAEMA
                     RAHREVTDRIRIIRRLDFTKKHRVDTTYEEHARILRAILAQRSDEACRLLAAHIGASH
                     AEVRKITLHEIYEARHAGRAEAST"
     gene            1874..2797
                     /locus_tag="VGC69_11540"
     CDS             1874..2797
                     /locus_tag="VGC69_11540"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295953.1"
                     /translation="MKHYTVAQMEAFLAISESGSFRIAADRLGITQPSISLRIRELET
                     ALGCALFLRGKGGVRLSEAGQIALQYVQRGFDIFQEMERRLSSGDPLTGSLRLGASNT
                     FALSCLPILLAALEQLHPTLRVELTINNSGALRALLDAQKLDIAFLVDAPAGPHYVVE
                     PLASCEIAWFSRAAPGGTRALRARDLLDRRIVTMPPSSPFHTVISDWFARAKVPLPTL
                     DICNDMATLVRLVRLGVAVSVLPASLVDHEMTLGGMVRHRTVPPLEPLVIYAAHRAKA
                     RGRASRVIIDIARDAIANAGPSMTPLPAPGA"
     gene            complement(2821..3699)
                     /locus_tag="VGC69_11545"
     CDS             complement(2821..3699)
                     /locus_tag="VGC69_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEY0295954.1"
                     /translation="MSDSSKRRAAFRALVERRQGLLMPGAFNALSARVAADAGFQSLY
                     LSGAGVTNMYLGMPDLAFIGLTDMADHTARVRDAVSLPLLVDADTGYGNAVNVGFAVR
                     TLERAGADAIQLEDQVSPKKCGHFNGKGVIETAEMLGKIRAAVDAREDPNTLIIARTD
                     ACAVHGFEAAIERAHAFSEAGADVLFVEAVESLAEIEKLPGLLPRPLLMNIVIGGKTP
                     ALPQARLAELGFGIVLYANAALQGAVLGMQKAFAALGSTGMLEENPELVVPFQERQRL
                     VNKPLYDKLEKQYAAP"
     gene            complement(3771..5084)
                     /locus_tag="VGC69_11550"
     CDS             complement(3771..5084)
                     /locus_tag="VGC69_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0295955.1"
                     /translation="MPQENLRPDTIAERAVIKKIAWRFLPVLILGYLLNYLDRTNIGF
                     AALTMNKDIGLSSTQFGFGAGVFFLAYCLFEIPSNILLFRVGARLWLARIMITWGIFS
                     AVMALVVDPYSYYGARLLLGLSEAGFFPGVTFFLSSWFPAKYRTSILAWFLLAIPLSS
                     LIGSPLSGSLLYLDGTWGLSGWKWMFLLEGTPTALVGIWALFVLSDSPAKATWLNAQE
                     RALITGMLEGEKREKPRKNFAAALSDPRVHLLALIQFGFTVGSYGVGIWLPLILKQFL
                     STPLEIGLVSAIPYIFASVGMLLWARYSDRSGNSLGNLAWSCALGAVGLAFSVFAHST
                     VSSMVGLTLSLVGVTAARAIFWAIPGRIMTGIGAAAGFAYINCIAALGGFAGPYVMGW
                     ARDKTGNFSAGLALMGLILLVTVLLVLPLHYYGKRARTDGAVVTP"
     gene            complement(5132..5977)
                     /locus_tag="VGC69_11555"
     CDS             complement(5132..5977)
                     /locus_tag="VGC69_11555"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0295956.1"
                     /translation="MQPNIPAHAPAPAPQPGGVQRVDCHAHIMVRGAALAPGRHSEPK
                     RDISAEDFLALLDAHGITHGVLTQPSFYGTDNSLLLDALRRHPGRLRGTAIVDFGIAD
                     AELAALHAGGVRGLRLNWLRRDDLPDPAGAPCQRLFGRAREHGLHIELYIEGRKMARV
                     LPAIRRSGAAVVVDHFGSPEPDTGIAGAGFQEVLEGLRAGDTWVKLSAPYRLGGAPAG
                     PYARALLEAGGPGQLLWGSDWPWVSHEDTQRYEQCLADFERWIPDPAQRAEIWRDTPR
                     RVFGF"
     gene            complement(6190..7053)
                     /locus_tag="VGC69_11560"
     CDS             complement(6190..7053)
                     /locus_tag="VGC69_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0295957.1"
                     /translation="MPIIHPLSYRLDQAPAQTPAPLVLDSPHSGTAYPPDFASSADFG
                     ALRTAEDTWVDDLWGGAVDQGVPLLGATCPRAYIDVNRGLEDIDPTLLDAPWPGPLSD
                     SPKVKLGKGLIWRMLDDGLPLYDRKLSVAEVQYRIDACWRPYHAAVRKLLDEAHARLG
                     KVWHINCHSMPSVAGAYATGAPGAAHPDFVLGDRDGTTADPAFAAFVAGWLRARGYDV
                     AVNDPYKGVELVRAYSNPAQGRHSLQIEVNRKLYMDEVTLRPNAGYGKLKQDLRDLTT
                     ALLGWMQPQLG"
     gene            complement(7057..8043)
                     /locus_tag="VGC69_11565"
     CDS             complement(7057..8043)
                     /locus_tag="VGC69_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295958.1"
                     /translation="MHRRHLVLGLSLAATLACAPLSAAQAQDDYPSKPIRLVIPFPPG
                     GTTDIVGRLFAEKLGRELGRAVVVENRGGAGGSIGSAVVAQAAPDGYTLGIATVSTHG
                     INPAVYPKLPFDVVKDFTPISNLAAVPNIMTVNPDKVPARNIAEFIQLAKSQPGKFTY
                     ASAGNGSVSHMMGELFKMTAGVNLVHVPYRGVGPALNDTLSGQVDILFDNLPSSLPHA
                     QSGKLVAMAVASPKRVAALPDVPTFAEAGLAPVNDASWFGLVAPAKLPQPILDRLYQA
                     VVKVSAQPDVVKTLESLGAEPVANTPAEYARQIADEVAKNRRIAQQAHIQVD"
     gene            8227..9144
                     /locus_tag="VGC69_11570"
     CDS             8227..9144
                     /locus_tag="VGC69_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0295959.1"
                     /translation="MRRFCPSLADLRAFEVAARHCNFTRAAQELCITQGAVSKQVKHL
                     EAFLGVLLFARGRQGLVLTEAGRGYLEKVQAGLGQIEAATVELIAHQGQGGTLHLTSM
                     PTFGARWLIPRLAAFMRLRPDIHLEFLPHRQGYDFSAPELDAAVRFGEGAWPGSGADY
                     LVGRDVVPVCSPRLVPSAQAGAADLLAYPLLHHTSAPDGWSDWFAQAGCDTRRAPEGA
                     RFDQYALLSQAAAAGFGIALIPRCLVEEELRDGRLAVPVALPILARQGYYLCYPQQKA
                     SLPALQAFRGWLKSVSLEAEPRLPGAAGA"
     gene            9171..10304
                     /gene="mnmA"
                     /locus_tag="VGC69_11575"
     CDS             9171..10304
                     /gene="mnmA"
                     /locus_tag="VGC69_11575"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0295960.1"
                     /translation="MSSLTPKSKKGRVVVGMSGGVDSSVTAWLLKQQGYEVVGLFMKN
                     WEDDDDSEYCSTRQDLLDAASVADRVGVEFEYVNFAAEYKDRVFADFLREYSAGRTPN
                     PDVLCNAEIKFKAFLDHAMSLGAECIATGHYARVRRVDGAGGPEYQLLKALDASKDQS
                     YFLHRLNQAQLSRTLFPLGEIKKTEVRRIAHEIGLHNAAKKDSTGICFIGERPFREFL
                     NRYLPTAPGPILTPEGQKVGMHHGLSFYTLGQRKGLGVGGVKGRQNDDGTAEAWYAAR
                     KDLDKNTLYVVQGHEHPWLLSDTLRAQDASWVAGRPPAAGAGPARYGAKTRYRQADAA
                     STLAEAQGETFSLAFGQPQWAVTPGQSAVLYDGDICLGGGIIS"
     gene            10530..11525
                     /locus_tag="VGC69_11580"
     CDS             10530..11525
                     /locus_tag="VGC69_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0295961.1"
                     /translation="MKTPGWVWKTVGVLAAAWGLGTAPAAAQNGGWRPDHPVRIVVPF
                     PPGGTADFMARALAARLSGELGQQFIVDNKPGAAAALAATTVAHASPDGYTLLFANSA
                     ALVNNRYMFKNLGYDPDAFTPVALLGTAPLVLLANVDTPFKTVPDLMKYAHAHQDSMS
                     FASFGTGTLSHLTMELLDSREGVKLEHIPYKGEAEAMTALMGGQIKVFVSTLTSSQAS
                     IRAGKVRALGVTSQARTALLPGVPSIAEQGYAGFDLVTWFGIDAPPGTPAPVVDALSR
                     AVAKLNALPDYRQELARIGVEAPAGDSSPAALVAKMKSDMPVMQQLIKGAGIQPQ"
     gene            complement(11600..12211)
                     /locus_tag="VGC69_11585"
     CDS             complement(11600..12211)
                     /locus_tag="VGC69_11585"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0295962.1"
                     /translation="MKLIGSLTSPYVRKVRIVMAEKKLDYQLEIESVWSPDTQIQKYN
                     PLGKVPCLVMEDGGALFDSRVIVEYLDTLSPVARLLPQSGRERAAVKCWEAIADGILD
                     ACLIIVKERQRPAAQQSPEWIERQYGKVHASLDAMDKSLGDHQYCMGINYSLADTAAG
                     CAMGYLDLRFPDLDWRADHANLSRLYDKLSARASFVDTAPPKD"
     gene            12528..13904
                     /gene="purB"
                     /locus_tag="VGC69_11590"
     CDS             12528..13904
                     /gene="purB"
                     /locus_tag="VGC69_11590"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY0295963.1"
                     /translation="MQIAQALTELNALSPLDGRYASRGDALRGLLSEAGFMAHRVEVE
                     VAWLIALSDAGLPELPAFSQGERARLGELVSAFTEDDARRIKEIERVTNHDVKAVEYW
                     LKEKVADDAELSRAAEFIHFACTSEDINNTSHALMLARARSQVLVPRLNQLAGKLKEM
                     AVAYSAQPMLSRTHGQPASPTTLGKEMANVAARLQRAIAAIEAVEPLAKMNGATGNYN
                     AHTSAYPEIDWPEFSRRVLAGLGLTQNRHTIQIEPHDWMSALFDAVSRANIVILDLDR
                     DIWGYVSLGYFKQRLKEGEVGSSTMPHKVNPIDFENSEGNLGLANAVLRHLADKLPVS
                     RWQRDLTDSTVLRNLGVGLGYSLVAWDSCLRGLDKLEVNLAGIDADIDACWEVLAEPV
                     QTVMRRYGLPQPYEQLKALTRGKGITEEALREFIGGLALPDEPKQRLLAMTPRSYVGL
                     AVELARTV"
     gene            13941..14384
                     /locus_tag="VGC69_11595"
     CDS             13941..14384
                     /locus_tag="VGC69_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0295964.1"
                     /translation="MQRIAAAIGLTLAAFVAVPAQAQFAKPEDAVKYREAAMTMISSH
                     FGRLVAAARGSSPSDPAQVKNNAQVLQTLAALPWAAYGPGTEGGHAKPEVWSNAADFK
                     QKQADFTTHVAALSAAAETGDLAKLREAVGAVGASCKACHSSYRE"
     gene            complement(14372..15310)
                     /locus_tag="VGC69_11600"
     CDS             complement(14372..15310)
                     /locus_tag="VGC69_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295965.1"
                     /translation="MPIDRSGLSGVATFLAVAECLSFRQAALRLELSPTAVSKAVRQL
                     EKRHGVVLFQRTTRSVKLTEAGEALYRRLQPAAAVMNEAFTELGQYQQQPHGTLRLTL
                     SRSSMRTFAEPVIAAFRQRYPEVALELSLDDGFADLAAGGFDAGIRLGEAIAQDMVAV
                     RLTPEVTWAVMGSPGYFRQHGLPETPEDLMAHEAIQYRFVGSGQIHAWEFSRDGQPLK
                     VEMKGQLTVNDRASLLTLACRGLGLAYVSDREAADDLAAGRLQAVLRDYAPADGGLYL
                     YFPARMQMQPKLRAFIDLATSAPAATRGRQNQRYSR"
     gene            15458..16306
                     /locus_tag="VGC69_11605"
     CDS             15458..16306
                     /locus_tag="VGC69_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0295966.1"
                     /translation="MPRTWFITGTSSGFGRLLTEKLLERGERVAATLRTPAALDDLKS
                     RYADLLWVERLDVTDTSAIHRVVDQAFADLGRIDIVVNNAGYALFGAAEELSPEQIRQ
                     QIGVNLIGSIEVTQAVLPHLRAQGGGRILQVSSMGGQISLPALSLYHATKWGIEGFFE
                     SVIPEVAPFGIEITLVEPAGAKTNFAHGSMVHAEPLAAYDDTPVGIWRKARAAGDYHP
                     TLDPEKTVLAMIGSAAQHPAPKRLTLGSGAYELVHSGLTERLGALEAQKQVALDADYD
                     AAIGGD"
     gene            complement(16320..16997)
                     /locus_tag="VGC69_11610"
     CDS             complement(16320..16997)
                     /locus_tag="VGC69_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY0295967.1"
                     /translation="MTAMRIWDLPTRLFHWCFAACIVGAYVSVKLGGLYMDWHVRFGL
                     AALGLVIFRVVWGFIGPRHARFCSFVRGPGAVLAYLRELAAGTARSAGHNPLGALSVV
                     AMLADLGAQAVLGLFTSDDVMVQGPLYNFVSESTSSLLTSLHRANEWIIIALVVLHLL
                     AVAWHTHKKEPLVRAMVTGDMPADHLPGQAQASTDTAGVRLRALALAVIVAIFVLWIR
                     SLGAAAA"
     gene            17146..18210
                     /locus_tag="VGC69_11615"
     CDS             17146..18210
                     /locus_tag="VGC69_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0295968.1"
                     /translation="MSRSTLMHDKNFHVLLAIVSAAFVWLLWPFWGAVFWGTILAIIF
                     SPVQRRLVARFGGRRNLAALATLLLVLVVAILPLAFIAGSLVSEGAALYQRMRSGNLD
                     FGAYFQQALAALPTWMHDLLSRFNLADLDSLREKLSDGAMQVSRFLATQAFNIGQDTF
                     QFLINLGIMLYLLFFLLRDGSRLARRMLRAMPLSDGHKEPLFIKFTTVVRATVKGNVA
                     VAAAQGVLGGFIFWALGIQGALLWGVVMGFLSLLPAIGSALIWVPVAAYFLLTGALWQ
                     GVVLVLFCVLVIGMVDNLLRPLLVGKETKLPDYVILISTMGGMALFGLNGFVIGPLIA
                     ALFIASWDLFSPHEDGEAGS"
     gene            complement(18235..18564)
                     /locus_tag="VGC69_11620"
     CDS             complement(18235..18564)
                     /locus_tag="VGC69_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEY0295969.1"
                     /translation="MTLIRLAALYALTALAEIAGCYLVWLALVQHRSAWLLLPAALSL
                     AAFAWLLTWHPAAAGRVYAAYGGMYIVMALAWLRVVDGVALSRWDLAGAVLALAGMAV
                     IAWQPAA"
     gene            complement(18561..>19715)
                     /locus_tag="VGC69_11625"
     CDS             complement(18561..>19715)
                     /locus_tag="VGC69_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295970.1"
                     /translation="SCNAVVKAFGAETREEARLARVLGKWRQRTRRTWVRGTRNGGIQ
                     GLMLTAMRASLLGAALLLWKSGAAGAGDITFALTMFFVLQGYMRDIGMHIRNLQRSIN
                     DMEELVIMERQPLGIADAPGARPITITQGEIRFEHVTFHYGNHVLPLYQDFSTRIAPG
                     ERVGLVGHSGSGKTTFIKLIQRLYDLNGGRITIDGQDIAQVQQASLRGQIAIVQQEPI
                     LFHRTLSENIAYARPGASRAQIEQAARQANAHEFIETLPRGYDTLVGERGIKLSGGER
                     QRVAIARAFLADAPILIFDEATSSLDSESEALIQQAMERLMAGRTTLVVAHRLSTVRA
                     LDRLLVMEHGRIAEEGSHDALVRMPGGLYRRLFERQALELTKGLAAETLA"
BASE COUNT         3067 a         6608 c         6853 g         3187 t
ORIGIN      
        1 gggctgccac gcgcgcgccg tgcagccggt ggcggacctg ctgccgcggg gcgcgcgcgg
       61 cttgcgcatc ggcgtgctcg gcggctattt cgcgcaatat gccggcccgg ccgcgagcga
      121 ggccgtgcgc ctggcggccg gcgcgctggg cgccgtcgcc acggtcgatt ggcccgacgc
      181 ggcgctgggg cgcgccgcgg ccttcatcgt cacggcgggc gaggggggca gcctgcactt
      241 gcccgacctg cgcacccgcg cggccgattt cgaaccgctt tcggtggacc gcttcatggc
      301 cggcgccttg cagcccgcgc actgggtaac gcgcgcgcag cggtttcgcc gcgtctaccg
      361 cgaccgcgtc aacgcgctct tcgccgactg ggatgtgctg ctggccccgg ccacgccggt
      421 gccggcgccc gccatcggcg ccgagtggat agatatcaat ggcgaacgcc atctctgccg
      481 cccgtccatg ggcctgttga cccagcccgt ttccttcgcc ggctgcccgg tggtcgccgc
      541 gcccttgtgg ccggcggcag gacaaggcct gccgctgggt gtacaactga tagccgcgcc
      601 gtggcgcgaa gacctcgcct tgcgcgcggc ccatgccctg gagcaggccg gcatcgccgg
      661 cttgcggccc gcttttttcg cgtcaacgac actatgatgg acatcaacct gcccgatgtg
      721 ctggccgaag tgcaaaggca attcgaccgc tatgaaaagg ccttgaccgg caacgacgtc
      781 gccgtgctcg acgagttgtt ctgggacagt ccgcacacgc tgcgctatgg cgccaccgag
      841 aacctgtatg gctacgcgca gatccgggat tttcgcgcgg cgcgtccgtc ccaggggctg
      901 gcgcgcgagg tgctgaagac ggtcatcacc acctatggcc gcgatttcgc cacctgcaat
      961 atcgaattcc gccgcgcggg cgtgccgggc accgggcgcc agagccaggc ctgggtgcgc
     1021 acgccggccg gctggcgggt ggtcgcggcc cacgttagca tgctgcgcta agcctgcgcc
     1081 gcttcccgcg ccgcccgcgt ccgccaccga gcttttcatc gtgcaaccca tccctccgcc
     1141 ccagcccaag gccgccatcg tccaccgcac gcgggccgac gacgtctacg acctgctcaa
     1201 gcgcgacatc gccgaattcc acttggtgcc tggcgaccgc ttcaccgagg gcgagatcag
     1261 cgaacgcctg ggcgtgtcgc gcacgcccgt gcgccaggct ttgttccgcc tgcagcagga
     1321 gggctacgtc gaggtgctgt ttcgcagcgg ctggcgggtg ctgcccttcg acttcaagct
     1381 gttcgagcag ctctacgatt tccgcctggt gctggagacg gaagcggtgc ggcgcctgtg
     1441 caccatgggc gcggaagcgg cgcggcaggc cctggccggc ctggaggccg tctggctggt
     1501 gccccccgac gcgcgcagca ccgacatggc ccaggtgggg cgctgggacg aggctttcca
     1561 ttgcagcctg ctcgacgcct cgggcaacgc ggagatggcg cgcgcgcacc gcgaggtgac
     1621 cgaccgcatc cgcatcatcc ggcggctgga cttcaccaag aagcatcgcg tggacaccac
     1681 ctacgaagag cacgcgcgca tcctgcgcgc catcctggcg cagcgctccg acgaagcctg
     1741 ccgcctgctg gccgcgcaca tcggcgccag ccacgccgaa gtgcgcaaga tcacgctgca
     1801 cgagatctac gaagcccgcc acgcggggcg ggccgaggcg tccacctgag cccggttcga
     1861 tgaagcgcta tcgatgaaac actacaccgt cgcccagatg gaggcttttc tcgcgatcag
     1921 cgagtcgggg tcgttccgca tcgcggcgga ccggctgggc atcacccagc cgtcgatctc
     1981 gttgcgcatc cgcgagctcg aaaccgccct gggctgcgcc ctgttcctac gcggcaaggg
     2041 cggggtgcga ttgtcggagg cggggcagat cgccttgcag tacgtgcaac gcggctttga
     2101 catattccag gaaatggagc ggcgcctgag cagcggcgac ccgctgacgg gctcgctgcg
     2161 gctgggggcg tcgaacacct ttgccttgag ctgcctgccg atcctgctgg cggcgctgga
     2221 gcaactgcac ccgacgctgc gcgtggagct gaccatcaac aacagcggcg cgctgcgcgc
     2281 gctgctcgac gcgcagaagc tggacatcgc cttcctggtg gatgcgccgg ccggcccgca
     2341 ttacgtcgtc gagccgctgg cctcgtgcga gatcgcctgg ttctcgcgcg ccgcgccggg
     2401 cggcacccgc gcgctgcgcg cgcgcgacct gctcgatcgg cgcatcgtca cgatgccgcc
     2461 ttcctctccg ttccacacgg tcatcagcga ctggttcgcg cgggccaagg tgccgctgcc
     2521 cacgctggac atctgcaacg acatggcgac gctggtgcgg ctggtgcgcc tgggcgtggc
     2581 ggtcagcgtg ctgccggcca gcctggtgga ccacgaaatg accttgggcg gcatggtgcg
     2641 gcaccgcacg gtgccgccgc tcgaacccct ggtgatctac gccgcgcacc gcgccaaggc
     2701 gcgcggccgc gccagccgcg tcatcatcga catcgcgcgc gacgcgatcg ccaacgccgg
     2761 cccctcgatg acgccgctgc cggcgccggg ggcctgagcg cgggtccgat cgagccgttt
     2821 tcagggcgcc gcgtactgct tttccagctt gtcgtagagg ggcttgttca ccagccgctg
     2881 gcgttcctgg aagggcacga ccagttccgg gttctcttcc agcatgccgg tcgagccgag
     2941 cgccgcgaag gccttctgca tgccgagcac ggcgccctgc agcgcggcgt tggcgtacag
     3001 gacgatgccg aagcccagct cggccaggcg cgcctggggc agggccgggg tcttgccgcc
     3061 gatgacgatg ttcatcagca gcgggcgcgg cagcaggccg ggcagcttct cgatctcggc
     3121 cagcgattcc acggcctcga cgaacagcac gtcggcgccg gcctcgctga aggcgtgggc
     3181 ccgctcgatg gcggcctcga agccgtgcac cgcgcaggcg tcggtgcggg cgatgatcag
     3241 cgtattgggg tcttcgcgcg cgtcgacggc ggcgcggatc ttgcccagca tctcggccgt
     3301 ctcgatcacg cccttgccgt tgaagtggcc gcacttcttg gggctgactt ggtcttccag
     3361 ctggatggcg tcggcgccgg cccgttcgag cgtgcgcacc gcgaagccga cgttcaccgc
     3421 gttgccgtag ccggtgtcgg cgtcgaccag cagcggcagc gagacggcgt cgcgcacccg
     3481 ggcggtgtgg tcggccatgt cggtcaggcc gatgaaggcc aggtcgggca tgcccaggta
     3541 catattggtg acgcccgcgc cgctcaggta gagcgactgg aagccggcgt cggccgcgac
     3601 gcgggcgctc agggcgttga aggcgccggg catcagcagg ccctggcggc gttcgaccag
     3661 cgcgcggaaa gcggcgcggc gcttggatga gtctgacatg attgaaatcc tcctggtggt
     3721 tcgcgggtgg ttaggtcagt gcgatcaggc gcatggtatg cccgaagtcc ttacggcgtc
     3781 acgacggcgc cgtcggtccg ggcccgcttg ccgtagtaat gcaggggcag caccagcagg
     3841 acggtcacca gcaggatcag tcccatgagc gccagcccgg ccgagaaatt gccggtcttg
     3901 tcgcgcgccc agcccatgac gtaggggccg gcgaagccgc cgagcgcggc gatgcagttg
     3961 atgtaggcaa agcccgcggc cgcgccgatg ccggtcatga tgcggcccgg gatggcccag
     4021 aagatggcgc gcgcggcggt cacgccgacc agcgacagcg tcaggcccac catcgagctt
     4081 acggtggaat gggcgaacac cgagaaggcc aggcccaccg cgcccagcgc gcagctccag
     4141 gccaggttgc ccagcgaatt gccgctgcgg tcggaatagc gcgcccacag cagcatgccg
     4201 acgctggcga aaatgtaggg gatggccgac accaggccga tctccagcgg ggtggagagg
     4261 aattgcttga ggatcagcgg cagccagatg cccacgccgt aggagccgac cgtgaagccg
     4321 aactggatca gcgccagcag gtgcacgcgc gggtcgctca gggcggcggc gaaattcttg
     4381 cgcggcttct cgcgcttttc gccttccagc atgccggtga tgagggcgcg ctcctgcgcg
     4441 ttgagccatg tggccttggc ggggctgtcc gacagcacga acagcgccca gatgccgacc
     4501 aacgcggtcg gcgtgccttc cagcaggaac atccacttcc agccgctcag gccccaggtg
     4561 ccgtccaggt agagcagcga acccgacagc gggctgccga tcagcgagga cagcgggatg
     4621 gccagcagga accaggcaag aatgctggtg cggtacttgg cggggaacca gctcgacagg
     4681 aagaacgtga cgccggggaa gaagccggcc tcggacaggc cgagcagcag gcgcgcgccg
     4741 tagtagctat aggggtcgac caccagcgcc atgaccgccg agaaaatgcc ccaggtgatc
     4801 atgatgcggg ccagccacag ccgcgcgccg acgcgaaaca gcaggatgtt gctgggtatc
     4861 tcgaacaggc agtaggccag gaagaagacc cctgccccga agccgaactg ggtggaggag
     4921 agcccgatgt ccttgttcat ggtcagggcg gcgaagccga tgttggtgcg atccaggtag
     4981 ttcagcaggt agcccaggat cagcaccggc aggaagcgcc aggcgatttt cttgatgacg
     5041 gcccgttccg cgatcgtgtc gggtcgtaga ttctcttgcg gcatggtttg tctccttatg
     5101 ggcaatggtt gttttgtgta tcggggcggc gtcagaagcc gaacacccgg cgcggcgtgt
     5161 cgcgccagat ctcggcgcgc tgcgccggat cgggaatcca gcgctcgaag tcggccaggc
     5221 attgctcgta gcgctgggtg tcctcgtgcg agacccaggg ccagtcgctg ccccacagca
     5281 gctgcccggg gccgcccgct tccagcagcg cccgcgcgta gggtccggcg ggcgcgccgc
     5341 ccagccggta gggggccgag agcttgaccc aggtgtcgcc ggcgcgcagg ccctcgagca
     5401 cttcctggaa gcccgcgccc gcgatgccgg tgtccggctc gggcgagccg aaatggtcca
     5461 ccaccacggc cgcgccgctg cggcggatgg ccggcagcac ccgcgccatc ttgcgcccct
     5521 cgatgtagag ctcgatgtgc agcccgtgtt cgcgggcgcg gccgaacagg cgctggcagg
     5581 gcgcgcccgc ggggtcgggc aggtcgtcgc ggcgcagcca gttcaggcgc agcccgcgca
     5641 cgccgccggc atggagcgcc gccagttccg cgtcggcgat gccgaagtcc acgatggcgg
     5701 tgccgcgcag gcgtccgggg tggcgccgca gcgcgtccag cagcagcgag ttgtcggtgc
     5761 cgtagaagct gggctgcgtc agcacgccgt gcgtgatgcc gtgcgcatcg agcagggcca
     5821 ggaagtcttc ggcgctgatg tcgcgcttgg gttcggagtg gcggccgggc gccagcgcgg
     5881 cgccgcggac catgatgtgg gcgtggcaat ccacgcgttg gacgccgccg ggttgcgggg
     5941 cgggcgccgg cgcgtgggcc gggatattgg gctgcatgga ggggactgtc tctggtttct
     6001 gtcttggtcc cgcgcgcctc gccgtggccg gcctgggcgc gccatcggga tcgcgcgctg
     6061 ccggttccgg cggcgcgcgg gtatgccgat gactctacgt accctcaaat catttgtcta
     6121 attgatactt cttgagaaaa tcaataggaa tttcagatga cttggttcgg atggcctggc
     6181 tcggacaagc taaccgagct gcggctgcat ccagcccagc agggccgtcg tcaggtcgcg
     6241 cagatcctgc ttgagcttgc cgtagccggc gttgggccgc agcgtcacct cgtccatgta
     6301 cagcttgcgg ttgacctcga tctgcaggct gtgccggcct tgcgccgggt tgctgtaggc
     6361 ccgcaccagc tccacgccct tgtaggggtc gttgaccgcc acgtcgtagc cgcgcgcgcg
     6421 cagccagccg gccacgaacg cggcgaaggc cgggtcggcc gtggtgccgt cgcggtcgcc
     6481 cagcacgaag tcgggatggg ccgcgccggg cgcgccggtg gcataggccc cggccacgct
     6541 gggcatggag tggcagttga tgtgccatac cttgcccagg cgcgcgtggg cctcgtccag
     6601 cagcttgcgc accgcggcgt ggtaaggccg ccagcaggcg tcgatgcggt actgcacttc
     6661 ggccacgctc agcttgcggt cgtacagggg caggccgtcg tcgagcatgc gccagatcag
     6721 gcccttgccc agcttgacct tgggcgagtc cgagagcggg ccgggccagg gtgcgtccag
     6781 cagggtcggg tcgatgtctt ccaggccgcg gttcacgtcg atgtaggcgc gcgggcaggt
     6841 cgcgcccagc aagggcacgc cctggtccac ggcgccgccc cacaggtcgt cgacccaggt
     6901 gtcctcggcc gtgcgcagcg cgccgaaatc ggcggagctg gcgaagtcgg gcgggtaggc
     6961 cgtgccgctg tgcggcgaat ccagcaccag cggcgcgggc gtttgcgcgg gcgcctggtc
     7021 caggcggtag gaaagcggat ggatgatggg catgtctcaa tctacctgga tatgggcttg
     7081 ctgggcgatg cggcggttct tggccacttc gtcggcgatc tgccgggcgt actcggccgg
     7141 ggtgttggcc acgggctcgg cgcccaggct ttcgagcgtc ttgaccacgt cgggctgggc
     7201 gctgaccttg accacggcct ggtagagcct gtcgaggatg ggctgcggca gcttggccgg
     7261 cgccaccagg ccgaaccagg aggcgtcgtt gaccggcgcc aggccggctt cggcgaaggt
     7321 gggcacgtcg ggcagggcgg ccacgcgctt gggcgaggcc acggccatgg ccaccagctt
     7381 gcccgactgg gcgtgcggca gcgacgaagg caggttgtcg aacaggatgt ccacctggcc
     7441 ggacagcgtg tcgttgagcg ccgggcccac gccgcggtag ggcacgtgca ccagattcac
     7501 gcccgcggtc atcttgaaga gttcgcccat catatgggag accgagccgt tgccggccga
     7561 ggcataggtg aacttgccgg gctggctctt ggccagctgg atgaattcgg cgatgttcct
     7621 ggcgggcacc ttgtcgggat tgaccgtcat gatgttgggc acggccgcca ggttggagat
     7681 gggcgtgaag tccttcacca cgtcgaaggg cagcttggga tagacggccg ggttgatccc
     7741 gtgcgtgctc acggtggcga tgcccagggt atagccgtcg ggagcggcct gcgccacgac
     7801 cgcgctgccg atggagccgc cggcgccgcc gcggttctcg accaccacgg cgcggcccag
     7861 ttcgcggccc agcttctcgg cgaacaggcg gccgacgatg tcggtggtgc cgccgggcgg
     7921 gaaggggatg accaggcgga tgggcttgct gggatagtcg tcctgggctt gggcggcgga
     7981 caggggcgcg caggcgaggg tggcggccag cgacaggccc aggacgagat ggcggcgatg
     8041 catggaaggg atcccctttt gaatgtcggt tcatggcgca gggcgcgggt gccgcgcacg
     8101 actgcggggg cgattctggc gtccgcccgc gtttttgcac aaacgaaaga ttctccttaa
     8161 actattccgt ttactcatgg ctggacctgg ggctgaatct tgcaaacccc ggggccggcc
     8221 ggttccatgc gccgattctg tccgtccctg gccgacctcc gggctttcga ggtggcggcc
     8281 cgccattgca atttcacgcg cgccgcccag gagctgtgca tcacccaggg ggccgtcagc
     8341 aagcaggtca agcacctgga ggcattcctg ggcgttttgc tgttcgcccg cggcaggcaa
     8401 ggcctggtgc tgacggaggc tgggcgcggc tacctggaaa aagtgcaggc ggggctgggc
     8461 cagatcgagg ccgccaccgt cgagctgatc gcccaccagg ggcagggcgg caccctgcac
     8521 ctgaccagca tgccgacttt cggggcgcgc tggctgatcc cgcgcctggc ggcgttcatg
     8581 cggctgcgcc cggacatcca cctcgaattc ctgccgcacc ggcagggcta cgatttttcg
     8641 gcgcccgaac tcgacgcggc ggtgcgcttc ggcgaagggg cctggccggg cagcggggcg
     8701 gactacctcg tgggccggga cgtcgtgccg gtgtgcagtc cccggctggt gccatcggcg
     8761 caggcgggcg cggccgacct gctggcctat cccctgctgc atcacacctc ggcgccggac
     8821 ggctggtcgg actggttcgc ccaggccggc tgcgacaccc gccgcgcccc ggaaggcgcg
     8881 cgcttcgacc agtacgcatt gctgtcgcag gccgccgccg cgggcttcgg catcgcgctc
     8941 atcccgcgct gcctggtcga ggaggaactg cgcgacggcc ggctggccgt gcccgtggcg
     9001 ctgcccatcc tcgcgcgcca gggctactac ctctgctatc cgcagcaaaa ggccagcctg
     9061 cccgccctgc aggccttccg cggctggctg aagtcggttt ccctggaggc cgagccgcgc
     9121 ctgccgggcg cggccggggc ctgatacgca ttcgacttta aaatcccatc atgtcttccc
     9181 ttacccccaa gtccaagaaa ggccgcgtcg tcgtcggcat gtcgggcggc gtcgattcgt
     9241 cggtcaccgc ctggctgctc aagcaacagg gctacgaggt cgtcggcctg ttcatgaaga
     9301 actgggaaga cgacgatgat tccgagtact gctccacccg gcaggacctg ctggacgccg
     9361 ccagcgtggc cgaccgcgtg ggcgtggaat tcgaatacgt caatttcgcg gccgagtaca
     9421 aggaccgcgt cttcgccgat ttcctgcgcg aatactcggc cggccgcacg cccaaccccg
     9481 acgtgctgtg caatgccgag atcaagttca aggcctttct cgaccacgcc atgtcgctgg
     9541 gcgccgagtg catcgccacc ggccactacg cgcgcgtgcg ccgtgtcgac ggcgccggcg
     9601 gccccgagta ccagttgctc aaggcgctgg acgccagcaa ggaccagagc tacttcctgc
     9661 atcgcctgaa ccaggcgcag ctgtcgcgca cgctgttccc gctgggcgag atcaagaaaa
     9721 ccgaggtgcg ccgcatcgcc cacgagatcg gcctgcacaa cgcggccaag aaggactcca
     9781 ccggcatctg cttcatcggc gagcggccgt ttcgcgaatt cctcaaccgc tacctgccca
     9841 cggcgccggg ccccatcctc acccccgaag ggcagaaggt gggcatgcat catggcctgt
     9901 cgttctacac gctgggccag cgcaagggcc tgggcgtggg cggcgtgaag ggccggcaga
     9961 acgacgacgg caccgccgag gcctggtatg ccgcccgcaa ggacctggac aagaacacgc
    10021 tctacgtcgt gcaggggcac gagcacccct ggctcttgtc cgataccctg cgggcgcagg
    10081 acgccagctg ggtggccggc cgcccgcccg cggccggcgc gggcccggcc cgatacggcg
    10141 ccaagacccg ctatcgccag gccgacgccg cctctaccct ggccgaggcg caaggcgaga
    10201 ccttctcgct ggccttcggg caaccgcagt gggcggtgac cccgggacag tcggccgtgc
    10261 tgtacgacgg cgacatctgc ctaggtggcg gcatcatttc ctgaagggcc cgcaaagcta
    10321 ctgcgcggac cgatttcacg ccggtgatgc tcgccgtgca ctagcacggc tgcgcttctc
    10381 ggcgcgaact cggtccgctc gctacgcttt tcggaccctt caggccgtca ggcttataac
    10441 tttcctatat ttgcatatgg tgttcattcc tctgggatgg tgctgcaata tcgggtttta
    10501 tcgcattgat tttgctagag gaaaagaata tgaaaacgcc aggatgggtc tggaaaacgg
    10561 tgggtgtgct ggccgcggcc tggggcctgg gcacggcgcc cgccgcggcg cagaacggcg
    10621 gatggcggcc tgatcatccg gtccgcatcg tggtgccgtt cccgccgggc ggcacggccg
    10681 acttcatggc gcgggccctg gcggccaggc tgtcgggcga gctgggccag cagttcatcg
    10741 tcgacaacaa gccgggcgcg gcggcggcgc tggccgccac gaccgtggcc cacgcatcgc
    10801 cggacggcta tacgctgctg ttcgccaatt cggccgcgct ggtgaacaac cgctacatgt
    10861 tcaagaacct gggctacgac ccggacgcct ttacgcccgt tgccctgctg ggcacggcgc
    10921 cgctggtgct gctggccaat gtcgatacgc cgttcaagac ggtgcccgac ctcatgaaat
    10981 acgcgcacgc ccaccaggac tccatgagct ttgcctcgtt cggcacgggc accctgtcgc
    11041 acctgacgat ggaattgctc gacagccgcg agggcgtcaa gctcgagcac attccctaca
    11101 agggcgaggc cgaggccatg acggccctga tgggcgggca gatcaaggtg ttcgtgtcca
    11161 cgctcaccag cagccaggcg tccatccgcg ccggcaaggt gcgggcgctg ggcgtgacct
    11221 cgcaggcgcg cacggccctg ctgcctggcg tgcccagcat cgcggagcag ggctatgccg
    11281 ggttcgacct ggtgacctgg ttcggcatcg acgccccgcc gggcacgccg gcgccggtgg
    11341 tcgacgcgct gagccgcgcc gtcgccaagc tcaatgcctt gccggactat cgccaggagc
    11401 ttgcccgcat cggcgtcgaa gcgcccgcgg gagattcgag cccggcggcg ctcgtcgcca
    11461 agatgaagag cgacatgccg gtgatgcagc agttgatcaa gggcgcgggc atccagccgc
    11521 aatgaagcct tcttcggcaa gcccggtctg aaaagcacaa ggcccgcatg tcgcgggcct
    11581 tgtcgcatgc gccgtccgct cagtctttcg gcggggcggt gtcgacgaac gaggcgcggg
    11641 ccgacagctt gtcgtacagg cggctcaggt tggcatggtc ggcgcgccag tccaggtcgg
    11701 gaaagcgcag gtccaggtag cccatggcgc agccggcggc ggtgtcggcc aggctgtagt
    11761 tgatgcccat gcagtactgg tgatcgccca ggctcttgtc catggcgtcc aggctggcgt
    11821 ggaccttgcc gtattggcgc tcgatccatt cggggctttg ctgcgcggcg gggcgctggc
    11881 gttccttgac gatgatgagg caggcgtcga ggatgccgtc ggcgatggct tcccagcact
    11941 tgaccgcggc gcgctcgcgc cccgattgcg gcagcaggcg ggcaacgggc gagagggtgt
    12001 cgaggtattc gacgatgacg cgcgagtcga acagcgcgcc gccgtcttcc atgaccaggc
    12061 agggaacctt gcccagcggg ttgtatttct ggatttgcgt gtcgggcgac cagacacttt
    12121 cgatttcgag ctgatagtcc agctttttct cggccatgac gatccgtact ttgcggacgt
    12181 aaggactggt aagcgagccg atcagtttca tgaggtcaca gaattgcgaa ggtcacagaa
    12241 ttgcaaaggc aaagccggaa agcgggcgaa gtatagcagg gcgccccccg aaacaagaat
    12301 ggtcgcaagc ttggcggcat ccgtaacgat gcgccgccgc gacagtattc atagagggac
    12361 ggtgttaaaa tccggcgcgc cactaggcgt gcgccatgcc gcatgccgat aagtgcccga
    12421 caggccgccc gccggctgtc ctcccgcttt ttccagccac gggcgcccat cgccctggac
    12481 accattgggt acgggattcc gtacacacat ttcttcccat cgacaccatg cagattgccc
    12541 aagcgcttac cgaactcaat gccctgtcgc cgctggacgg ccgctatgcc tcgcggggcg
    12601 acgccctgcg cggcctgctg tccgaggccg gcttcatggc gcaccgggtc gaggtcgagg
    12661 tggcctggct gatcgccctg tccgacgcgg gcctgcccga gctgcctgcc ttcagccagg
    12721 gcgagcgcgc gcgcctgggc gagctggtgt cggcctttac cgaggacgac gcgcgccgca
    12781 tcaaggaaat cgagcgcgtc accaaccacg acgtcaaggc cgtcgaatac tggctgaagg
    12841 aaaaggtggc ggatgacgcc gagctgtcgc gcgccgccga gttcatccac ttcgcctgca
    12901 cctcggaaga catcaacaac acctcgcacg cgctgatgct cgcacgcgcg cgcagccagg
    12961 tgctggtgcc gcgcctgaac cagctggccg gcaaactcaa ggaaatggcc gtggcctact
    13021 cggcccagcc catgctgtcg cgcacgcacg gccagcccgc cagcccgacc acgctgggca
    13081 aggaaatggc caatgtggcc gcgcgcctgc aacgcgccat cgccgccatc gaagcggtcg
    13141 agcccctggc caagatgaac ggcgccaccg gcaactacaa cgcgcacacc tcggcctatc
    13201 ccgaaatcga ctggcccgag ttcagccgcc gcgtgctggc cggcctgggc ctcacgcaaa
    13261 accgccacac catccagatc gagccgcacg actggatgtc ggcgctgttc gacgcggtgt
    13321 cgcgcgccaa tatcgtcatt ctcgacctcg accgcgacat ctggggctat gtgtcgctgg
    13381 gctacttcaa gcagcgcctg aaggaaggcg aggtgggctc gtccaccatg ccgcacaagg
    13441 tcaatcccat cgatttcgag aactccgaag gcaatctggg cctggccaac gccgtgttgc
    13501 gccacctggc cgacaagctg cccgtgtcgc gctggcagcg cgacctcacc gattccaccg
    13561 tgctgcgcaa cctgggcgtg ggcttgggct acagcctggt ggcctgggat tcctgcctgc
    13621 gcgggctgga caagctggaa gtcaacctcg cgggcatcga cgccgacatc gacgcctgct
    13681 gggaagtgct ggccgagccg gtgcagaccg tcatgcgccg ctacggcctg ccgcagccct
    13741 acgaacagct caaggcgctc acgcgcggca agggcattac cgaggaagcc ctgcgcgaat
    13801 tcatcggcgg cctggccctg cccgacgagc ccaagcagcg cctgctggcc atgacgccgc
    13861 gttcctacgt gggcctggcg gtggagctgg ctcggacggt atagttgtcc gcggcgcctg
    13921 ccgcgccaag gagacctgcc atgcaacgta ttgcggccgc catcggcctt accctggcgg
    13981 cgttcgtcgc cgtccccgcc caggcgcaat tcgccaagcc ggaagacgcg gtcaagtacc
    14041 gcgaagcggc catgacgatg atctcgtcgc atttcggccg cctggtggcg gcggcgcgcg
    14101 ggtcttcccc gagcgacccg gcccaggtca agaacaatgc ccaggtgctg cagacgctgg
    14161 ccgcgctgcc ctgggcggcc tacggtccgg gcacggaggg tggccacgcc aagcccgagg
    14221 tctggagcaa cgcggcggac ttcaagcaga agcaggcgga tttcaccacg catgtcgcgg
    14281 ccttgtcggc cgccgccgag acgggcgatc tggccaagct gcgcgaggcc gtgggggcgg
    14341 tgggggccag ctgcaaggcc tgccacagtt cttatcgcga gtagcgctgg ttttgccgcc
    14401 cccgggtggc tgcgggggcg gacgtcgcca aatcgatgaa cgccctgagc ttgggctgca
    14461 tctgcatccg cgccgggaaa tacaggtaca ggccgccgtc ggcgggcgca tagtcgcgca
    14521 gcaccgcttg caggcgcccc gccgccagat cgtccgccgc ctccctgtcc gacacgtaag
    14581 cgagcccaag gccgcggcat gccagcgtca gcagcgatgc ccggtcattg acggtcagct
    14641 ggcctttcat ctccaccttc aagggctggc cgtcccggct gaattcccac gcatggatct
    14701 gcccggagcc gacgaagcgg tattggatgg cttcgtgcgc catcaagtct tcgggcgttt
    14761 cgggcaagcc gtgctgccgg aaatagccgg ggctgcccat gaccgcccag gtgacttccg
    14821 gcgtgagccg gaccgccacc atgtcctgcg cgatggcctc gcccagccgg atcccggcat
    14881 cgaagccgcc cgcggcgaga tcggcaaaac cgtcgtccag cgagagttcg agcgccacct
    14941 ccgggtagcg ctggcgaaag gccgcgatga ccggttccgc gaaggtccgc atgctggagc
    15001 gcgatagcgt caggcgcaag gttccgtggg gctgctgctg gtattggccc aattcggtga
    15061 acgcttcgtt catgaccgcc gccgccggct gcaggcgccg gtagagcgct tccccggctt
    15121 cggtcagctt cacgctgcgc gtggtgcgct ggaacaggac cacgccgtgc cgcttttcca
    15181 gttggcgcac cgccttgctg accgcggtcg gcgacagctc caggcgcagc gccgcctgcc
    15241 ggaagctgag gcactcggcc acggcaagaa aggtggcgac gccgctcagt ccggatcgat
    15301 cgataggcat aggttgagca ccggccattc agggataaga acccacgatt gagaactata
    15361 agtacacagt acatcaactt tataccggtt tattccagca atgaaggcgc gcatgatgcg
    15421 gacttccctg gtttcacaac gcctcaagga gtcttccatg ccccgtacct ggttcatcac
    15481 cggcacatcg tccggcttcg gccgcctatt gacggaaaaa ttgcttgaac gcggcgagcg
    15541 cgtcgcggcg accctgcgca cgccggctgc gctggacgac ctgaaaagcc gctacgccga
    15601 tctgctctgg gtcgagcggc tggacgttac cgacacctcg gcgatccatc gtgtcgtcga
    15661 ccaggcgttt gccgatctgg gccggatcga tatcgtggtc aacaatgccg gttatgcgct
    15721 gttcggcgcc gccgaggaac tgagccccga acagatccgc cagcagatcg gcgtcaacct
    15781 gatcggctcg atcgaggtca cgcaggccgt gctgccgcac ctgcgcgcgc agggcggggg
    15841 acgcatcctg caggtgtcgt cgatgggcgg gcagatcagc ctgccggcgc tctcgctcta
    15901 ccacgccacc aagtggggca tcgagggctt ttttgaatcg gtgatccccg aagtcgcgcc
    15961 gttcggcatt gaaatcacgc tcgtggagcc cgccggggcc aagacgaact tcgcccacgg
    16021 cagcatggtc catgccgaac ccctggccgc ctatgacgac acgccggtgg gcatctggcg
    16081 caaagcccgc gccgcgggcg actatcaccc gacgctcgat ccggaaaaaa ccgtgctggc
    16141 aatgatcggc agcgccgctc agcacccggc gcccaagcgg ctgacgctag gcagcggcgc
    16201 ctacgaactg gtgcacagcg gcttgaccga acggctgggc gcgctggagg cgcagaagca
    16261 agtcgcgctc gacgccgatt acgacgcggc catcggcggc gactgatctc cctgcggcgt
    16321 caagccgcgg cggcgcccag cgaccgtatc cagagcacga agatcgccac gatgacggcc
    16381 aatgccagcg cgcgcaggcg cacgccggcc gtgtcggtgg aggcctgcgc ctggcccggc
    16441 aagtggtcgg cgggcatgtc gcccgtcacc atggcgcgca ccagcggttc ctttttatgg
    16501 gtatgccagg ccacagccag caggtgcaga accaccaggg cgatgatgat ccactcgttg
    16561 gccctgtgca gcgaggtcag caggctcgag gtggattcgg atacgaagtt gtaaagcggt
    16621 ccctgcacca tgacgtcgtc gctggtgaag aggcccagca ccgcctgcgc gccgaggtcg
    16681 gccagcatgg ccacgaccga cagggcgccc agcgggttgt ggccggcgct cctggcggtg
    16741 ccggcggcca gctcgcgcag ataggccagc acggcgcccg gcccccgcac gaagctgcaa
    16801 aagcgcgcat ggcgcgggcc gatgaagccc cagaccaccc ggaagatcac caggcccagc
    16861 gcggccaggc cgaagcgcac gtgccagtcc atgtacaggc cgcccagctt gaccgagacg
    16921 taggcgccga cgatgcaggc ggcaaagcac cagtggaaga ggcgggtggg caagtcccag
    16981 atacgcatgg cggtcatggt ggcggggcgg ggagtggtgt ttggcggcaa tgtagcacgc
    17041 gcggcgggcc gttggcttgc gcgctcatct tttacgcggg cagcttgccg gctcccccgc
    17101 tcggggcagg caaaggatat gatcggcctt ctccgaggaa ggttcatgag cagatccacc
    17161 ctgatgcacg acaagaattt ccatgtcctg ctggccatcg tgtcggcggc ctttgtctgg
    17221 ctgctctggc cgttctgggg cgcggtgttc tggggcacga tcctggccat catcttcagt
    17281 ccggtgcagc gccgcctggt cgcgcgtttc ggcggccgcc gcaacctggc cgcgctggcg
    17341 acgctgctgc tggtgctggt ggtggccatt ttgccgctgg cgttcatcgc cgggtcgctg
    17401 gtcagcgaag gggcggcgct ctaccagcgc atgcgcagcg gcaacctgga tttcggcgcg
    17461 tatttccagc aggccctggc ggcgttgccg acctggatgc acgatttgct gtcgcgcttc
    17521 aacctggccg acctggacag cctgcgcgag aagctgagcg acggcgccat gcaggtgagc
    17581 cgtttcctgg cgacccaggc cttcaatatc gggcaggaca cgttccagtt cctcatcaac
    17641 ctgggcatca tgctctacct gctgttcttc ctgctgcgcg acggctcgag gctggcgcgc
    17701 cggatgttgc gggccatgcc gctctccgac ggccacaagg agccgctgtt catcaagttc
    17761 acgacggtgg tgcgcgccac ggtcaagggc aatgtggcgg tggcggcggc gcagggcgtg
    17821 ctgggcggtt tcattttctg ggcgctgggc atccagggcg ccttgctgtg gggcgtggtg
    17881 atgggcttcc tgtcgctttt gccggccatc ggctcggcct tgatctgggt gccggtggcg
    17941 gcctatttcc tgctgacggg cgcgctgtgg cagggcgtgg tgctggtgct gttctgcgtg
    18001 ctggtcatcg gcatggtgga caacctgctg cggcccctgc tggtgggcaa ggaaaccaag
    18061 ctgcccgatt acgtgatcct gatctccacc atgggcggca tggcgctgtt cgggctgaac
    18121 ggcttcgtga tcggaccgct gatcgccgcg ctgttcatcg ccagctggga cctgttttcg
    18181 ccgcatgaag acggcgaggc cgggtcgtaa tcccgggttg tcgccgggcg gactttacgc
    18241 ggccggctgc caggcgatca ccgccatgcc ggccagcgcc agcacggcgc cggccaggtc
    18301 ccagcggctc agcgccacgc cgtcgaccac gcgcagccag gccagcgcca tcacgatgta
    18361 catgccgccg taggcggcat acacgcgtcc cgcggcagcc gggtgccagg tcagcagcca
    18421 ggcgaaggcc gccagcgaca gggcggctgg cagcagcagc caggcgctgc ggtgctggac
    18481 cagggccagc cataccaggt agcagcctgc gatctcggcc agtgccgtca aggcatagag
    18541 ggcggccagc ctgatcagcg tcatgccagc gtctcggccg ccaggccttt ggtgagctcc
    18601 agcgcctgcc gctcgaacaa gcgccggtac agcccgcccg gcatgcggac cagcgcatcg
    18661 tggctgcctt cttcggcgat gcggccatgc tccatcacca gcagcctgtc gagcgcgcgc
    18721 accgtcgaca ggcgatgcgc caccaccagc gtcgtgcggc ccgccatcag gcgttccatg
    18781 gcctgctgga tcagcgcctc gctttcgctg tccaggctcg aggtggcctc gtcgaagatc
    18841 aggatgggcg cgtccgccag gaaggcccgg gcaatcgcca cgcgctggcg ttcgccgccc
    18901 gagagcttga tgccgcgctc gcccaccagc gtgtcgtagc cgcgcggcag ggtttcgatg
    18961 aactcgtgcg cgttggcctg gcgggccgcc tgctcgatct gcgcgcggct tgcgccgggg
    19021 cgggcatagg cgatgttctc cgacagcgtg cggtgaaaca ggatgggctc ctgctgcacg
    19081 atggcgatct ggccgcgcag cgaagcctgc tgcacctggg cgatgtcctg cccgtcgatg
    19141 gtgatgcgcc cgccgttcag gtcgtacagg cgctggatga gcttgatgaa ggtggtcttg
    19201 cccgagcccg aatgacccac caggcccacg cgctcgcccg gcgcgatccg cgtggagaag
    19261 tcctggtaga gcggcaggac gtgattgccg tagtggaagg tcacgtgctc gaagcggatc
    19321 tcgccctgcg tgatggtaat gggccgcgcg cccggcgcgt ccgcgatgcc caggggctgg
    19381 cgctccatga tcaccagttc ttccatgtcg ttgatcgaac gctgcaggtt gcggatatgc
    19441 atgccgatat cgcgcatata cccctgcagg acgaagaaca tggtcagcgc gaaggtgatg
    19501 tcgccggcgc cggccgcgcc gctcttccac agcagcaggg ccgcgcccag cagggatgcg
    19561 cgcatggcgg tgagcatcag gccctgtatg ccgccgttgc gggtgccccg cacccaggtg
    19621 cggcgcgtgc gctggcgcca cttgcccagc acgcgcgcca gccgggcctc ctcgcgggtt
    19681 tccgcgccga aggccttgac cacggcattg cagct
//
LOCUS       DASWPO010000217         5615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2013649, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000217 DASWPO010000000
VERSION     DASWPO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013649"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /gene="purH"
                     /locus_tag="VGC69_11630"
     CDS             <1..1398
                     /gene="purH"
                     /locus_tag="VGC69_11630"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0295971.1"
                     /translation="KTLHPKIHGGLLARRDSDEHMRTIAEHGIDRIDLLVVNLYPFRE
                     TIIKPDCTFADAVENIDIGGPAMLRAAAKNHGTEVGGVTVVIDPADYARVLDEIDEND
                     GTSYTLRLELAGKVYAHTAAYDGAIAAYLSSLKDATPEQNAVPERAEYPRVLTVQMTR
                     EQALRYGENPHQTAAFYVDVQRPVGLLAGYAQLQGKELSYNNIADADAAWDCVRSFSL
                     PACVIIKHANPCGVAVAVDALTAYRQAFKTDPTSAFGGIIAFNRRVDLATAEAVSKQF
                     MEVLLAPAYDDDALALLVAKKNVRVLVVSLGDGQNPYDVKRVGGGWLVQTPDAYDPPY
                     EGFKVVTSRKPTENEMNNLLFAWKVAKFVKSNAIVFAGNGLTLGVGAGQMSRIDSARI
                     AAIKAANAGLSLQGSAAASDAFFPFRDGLDVIVDAGATCVIHPGGSMRDDEVIAAAEE
                     RGIAMVLTGTRHFRH"
     gene            1406..1948
                     /gene="ruvC"
                     /locus_tag="VGC69_11635"
     CDS             1406..1948
                     /gene="ruvC"
                     /locus_tag="VGC69_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY0295972.1"
                     /translation="MRVLGIDPGLRRTGFGVIDLEGARLRYVASGTIVVPTDLPLPGR
                     LKVILDNLREVARETQPDVAALEIVFLNTNPASTLLLGQARGAALCALADSRLDVHEY
                     TALQIKKAVVGTGRAAKEQVQMMVQRLLSLNGTPAPDSADALACAICHAHVSPLQQRL
                     TQAQGSPSSRARVRAGRLRG"
     gene            2017..2589
                     /gene="ruvA"
                     /locus_tag="VGC69_11640"
     CDS             2017..2589
                     /gene="ruvA"
                     /locus_tag="VGC69_11640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY0295973.1"
                     /translation="MIGRITGTLLEKTPPTVCVDVNGVGYEIDVPMSTLYTLPETGAR
                     VTLYTHLVVREDAHLLFGFANASERGVFRLLIKVTGIGARIALAVLSGMSAAELAQAV
                     TLQETGRLTRVPGIGKKTAERLLLELRGKLGADIGATAHAVPDNQTDILNALTALGYS
                     DKESLASLKQLPEGVGVSEGIRIALKALAR"
     gene            complement(2605..3387)
                     /locus_tag="VGC69_11645"
     CDS             complement(2605..3387)
                     /locus_tag="VGC69_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295974.1"
                     /translation="MRRLRRMAGLAGAAGLGLALAGCVGPTALKPMLEDSEGYSARYF
                     QLDRLSPGGKAVLAPPGSEPLGFRRLTAKGRVQVSGTGQPDTHRIAVFERTLINDRND
                     GTVREIELRTVDGLPLLVLYGLSYRGVAQLAVQQGQPARNLALPILALRSSSSWKSMA
                     GLQENTAYDYVDSYGTGDPLMPSIKIERHCKSGTFYDAGKFLAGLPGQAIDLVCDDIN
                     SGGAKVSENRFVWLGQYGVVLPARLTTLNGITEMQYDSVTAQ"
     gene            complement(3522..4490)
                     /locus_tag="VGC69_11650"
     CDS             complement(3522..4490)
                     /locus_tag="VGC69_11650"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEY0295975.1"
                     /translation="MSLVLPTFADVEAAAARIAGFAHRTPVLTSSTADALAGARLFFK
                     CENFQRMGAFKFRGGYNAIAKLTPGQKQAGVLTFSSGNHAQAIALAARLLGVQATIIM
                     PQDAPAAKRAATEGYGGKVVTYDRYQEDREAVARAIQEKTGATLIPPYDHEDVIAGQG
                     TAAKELFEEVGELDYLFAPLGGGGLLSGTLLSAGALSPSCKVYGVEPEAGNDGQQSLR
                     KGEVVHIQAPKTLADGAATTHLGNITFPIIKQRVTDIVTISDAQLVQAMKFFAERMKM
                     VVEPTGCLSAAAVLQQVVPVPAGARVGMILSGGNVDLAAYGKLLAG"
     gene            4688..>5615
                     /gene="ruvB"
                     /locus_tag="VGC69_11655"
     CDS             4688..>5615
                     /gene="ruvB"
                     /locus_tag="VGC69_11655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY0295976.1"
                     /translation="MAIQNDSLSALPDSTRLVAPQPVSPNEESIERALRPKALQEYVG
                     QARAREQLEIFIAAARGRREALDHVLLFGPPGLGKTTLAHIIAFEMGVNLRQTSGPVL
                     ERPGDLAALLTNLERNDVLFIDEIHRLSPVVEEILYPALEDFQIDILIGEGPAARSVK
                     LDLQPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYTAEELSRIVTRSAGLLNAAITPD
                     GAQEVARRARGTPRIANRLLRRVRDYAEVKAGGTIDTEVASRALAMLEVDPQGLDLMD
                     RKLLEAIVHKFDGGPVGVDSLAAAIGEERDTIE"
BASE COUNT          940 a         2058 c         1804 g          813 t
ORIGIN      
        1 aagaccctgc atcccaagat ccacggcggc ctgctggccc gccgcgacag cgacgaacac
       61 atgcggacca tcgccgagca cggcatcgac cgcatcgacc tgctggtggt gaacctctac
      121 cccttccgcg aaaccatcat caagccggac tgcacctttg ccgacgccgt cgagaacatc
      181 gacatcggcg gccccgccat gctgcgcgcc gcggccaaga accacggcac cgaagtcggc
      241 ggcgtcacgg tggtgatcga ccccgccgac tacgcccgcg tgctggacga gatcgacgaa
      301 aacgacggca cctcgtacac cctgcgcctg gagctggcgg gcaaggtcta tgcccatacc
      361 gccgcctacg acggcgcgat tgccgcctac ctgagcagcc tgaaggacgc cacccccgaa
      421 cagaacgccg tccccgaacg cgccgaatat ccgcgcgtgc tcaccgtgca gatgacccgg
      481 gaacaagccc tgcgctacgg cgagaacccg caccagaccg ccgccttcta tgtcgacgtg
      541 cagcgccccg tcggcctgct cgccggctac gcgcaactgc aaggcaagga gctctcgtac
      601 aacaacatcg ccgacgccga cgcggcctgg gactgcgtgc gcagcttcag cctgccggcc
      661 tgcgtcatca tcaagcacgc caacccctgc ggcgtggccg tggcggtcga cgcgctcacc
      721 gcctaccgcc aggccttcaa gaccgacccc acctcggcct tcggcggcat catcgccttc
      781 aaccgcaggg tcgacctggc cacggccgaa gccgtgagca agcagttcat ggaagtgctg
      841 ctggccccgg cctacgacga cgacgcgctg gcgctgctgg tcgccaagaa gaacgtgcgc
      901 gtgctggtcg tatccctggg cgacggccag aacccctacg acgtcaagcg cgtgggcggc
      961 ggctggctgg tgcagacgcc cgacgcctac gatcctccct acgaaggctt caaggtcgtc
     1021 acctcgcgca agcccaccga gaacgagatg aacaacctgc tgttcgcctg gaaggtggcc
     1081 aagttcgtca agtccaacgc catcgtgttc gccggcaacg gcctgacgct gggcgtgggc
     1141 gcgggccaga tgagccgcat cgactcggcg cgcatcgccg ccatcaaggc ggccaacgcc
     1201 ggcctctcgc tgcagggttc ggccgccgcg tcggacgcct tcttcccctt ccgcgacggc
     1261 ctggacgtga tcgtggacgc gggcgccacc tgcgtgatcc atcccggcgg cagcatgcgc
     1321 gacgatgaag tgatcgccgc cgccgaagag cgcggcatcg ccatggtgct caccggcacc
     1381 cgccacttcc gccattaagc cgcgcatgcg cgtactcggc atcgatcctg gcctgcgccg
     1441 caccggtttc ggcgtgatcg atctcgaggg cgcgcggctg cgctacgtgg ccagcgggac
     1501 catcgtggtc ccgaccgacc tgccgctgcc cgggcggctc aaggtgatcc tggacaatct
     1561 gcgcgaggtt gcccgcgaga cgcagcccga cgtggccgcg ctcgaaatcg ttttcttgaa
     1621 taccaacccg gcttcgaccc tgctgctggg ccaggcgcgc ggcgccgccc tgtgcgcgct
     1681 ggccgacagc cggctcgacg tgcacgaata caccgcgctg cagatcaaga aggccgtggt
     1741 cggcaccggc cgcgccgcca aggagcaggt gcaaatgatg gtgcagcgcc tgctgtccct
     1801 gaacggcacg cccgcccccg actcggccga cgccctggcc tgcgccatct gccatgccca
     1861 cgtcagcccg ctgcaacagc ggctgacgca ggcgcaaggc tcgcccagtt cgcgcgcgcg
     1921 cgtgcgggcc gggcggctgc gcggctgacc tagcgataga atgccggcat cagccgccct
     1981 caggcggcgc ccaccaagac aaccacgggc catcgcatga tcggacgcat caccggaacc
     2041 ctgctcgaga aaaccccgcc caccgtctgc gtggacgtca acggcgtggg ctatgagatc
     2101 gacgtgccca tgagcacgct ctacaccctg cccgaaaccg gcgcgcgggt cacgctctac
     2161 acccacctgg tggtgcgcga ggacgcgcac ctgctgttcg gcttcgccaa cgccagcgaa
     2221 cgcggcgtct tccgcctgct gatcaaggtc acgggcatcg gcgcgcgcat cgcgctggcc
     2281 gtgctgtcgg gcatgtcggc ggccgagctg gcccaggccg tcacgctgca ggagaccggg
     2341 cggctgacgc gcgtgccggg catcggcaag aaaaccgccg aacgcctgct gctggaactg
     2401 cgcggcaagc tgggcgccga catcggcgcc acggcccacg ccgtccccga caaccagacg
     2461 gacatactca atgcgttgac ggccctgggc tattcggaca aggaatcgct agcctcgctc
     2521 aagcagttgc ccgaaggcgt gggcgtatcc gagggcatcc gcatcgcgct caaggcgctg
     2581 gcgcgctgag gccgctgccc ggcgctattg cgccgtcacg ctgtcgtact gcatctccgt
     2641 gatgccgttg agcgtggtca gcctggcggg cagcaccacg ccgtactggc ccagccagac
     2701 gaagcggttt tccgatacct tggcgccgcc gctgttgatg tcgtcgcaga ccagatcgat
     2761 agcctgcccg ggcaggccgg cgaggaattt cccggcatcg tagaaggtgc ccgacttgca
     2821 atgccgctcg atcttgatcg acggcatgag cgggtcgccg gtgccatagg aatccacgta
     2881 gtcgtaggcc gtgttctcct gcaggccggc catgctcttc caggatgagc tgctacgcaa
     2941 ggccaggatg ggcagggcca ggttgcgggc gggctggccc tgctgtacgg ccaactgggc
     3001 cacgccgcga tacgacagcc catacaagac cagcagcggc aggccatcga ccgtgcgcag
     3061 ctcgatctcg cgcaccgtac cgtcgttgcg gtcgttgatc agggtgcgct cgaagacggc
     3121 gatgcggtgc gtatccggct ggccggtgcc cgatacctgg acgcggccct tggccgtgag
     3181 ccgcctgaag cccagcggct ccgaccccgg cggcgccagc acggccttgc ccccgggcga
     3241 caaccggtcc agctggaaat agcgcgccga atagccctcg ctgtcttcca gcatgggttt
     3301 caacgcggtg gggcccacgc agcccgccag ggccagcccc aggcccgcag cgccggccag
     3361 gccggccatg cgtcgcaatc gtctcatcct ctacacctcg cctcttgttt gaattcggga
     3421 aagcggcagc agtgtagcgg cgcctcgcgc cgaatggggg cgattccccg gccatgccgg
     3481 gttttttgga aaaaaatgaa tgggcggggg gcctgcgccg ctcagccggc cagcagtttg
     3541 ccgtaagcgg ccagatccac gttgccgccg ctcaggatca tgcccacccg cgcgccggcg
     3601 ggaacgggca cgacttgctg cagcaccgcg gcggccgaca ggcagccggt gggctcgacc
     3661 accatcttca tgcgctcggc gaagaacttc atcgcctgca ccagctgcgc gtcgctgatg
     3721 gtgacgatgt cggtcacgcg ctgcttgatg atgggaaagg taatgttgcc caggtgcgtg
     3781 gtggccgcgc catcggccag cgtcttgggc gcctggatgt gcaccacctc gcccttcctc
     3841 agcgactgct ggccgtcgtt gccggcctcg ggctcgacgc cgtagacctt gcacgacggg
     3901 ctcagcgcac cggccgacag cagcgtgccc gacagcagcc cgccgccgcc cagcggcgcg
     3961 aacaggtagt ccagctcgcc cacttcctcg aacagctcct tggccgccgt gccctggccg
     4021 gcgatcacgt cttcgtggtc gtagggcggg atcagcgtgg cgccggtctt ttcctggatg
     4081 gcgcgggcca cggcctcgcg gtcttcctgg tagcggtcgt aggtgacgac cttgccgccg
     4141 tagccctcgg tcgcggcgcg cttggccgcc ggcgcgtcct gcggcatgat gatggtggcc
     4201 tgcacgccca gcagcctggc cgccagggca atggcctgcg catggttgcc cgacgagaac
     4261 gtcagcacgc cggcctgctt ctgcccgggc gtgagcttgg cgatcgcgtt gtagccgccg
     4321 cgaaacttga aggcgcccat gcgctggaag ttctcgcact tgaagaacag ccgcgcgccc
     4381 gccagggcgt cggccgtcga cgaggtcagc accggcgtgc ggtgggcaaa gcccgcaatg
     4441 cgggcggcgg cggcttcgac atcagcaaaa gtaggcagga caagcgacat aacaatcctc
     4501 gatgacttga gcattgccgt cggcgggcag gggccatggc ggcgcctctg aggcggggaa
     4561 gcccgcgcag cgggccgagg gcgcctgatg gcgagcccga gcccgactca gcgaagcagc
     4621 catggcccct gcccgccgac ggcaatgcgg cttaagaacc ataaccaggg tagtgtacga
     4681 tcaccgcatg gccatccaga acgactcgct ctccgccctc cccgactcga cgcgcctggt
     4741 cgcgccgcag cccgtctcgc ccaacgaaga atccatcgaa cgggccctgc gccccaaggc
     4801 gctgcaggaa tacgtcggcc aggcccgggc acgagaacag ctggaaatct tcatcgccgc
     4861 cgcccgcggc cgccgcgaag ccctcgacca cgtcctgctc ttcggcccgc ccggcctggg
     4921 caagaccacc ctggcgcaca tcatcgcctt tgaaatgggc gtgaatctgc gccagacctc
     4981 cggccccgtg ctcgaacgcc ccggcgacct ggccgcgctg ctgaccaacc tggaacgcaa
     5041 cgacgtgctc ttcatcgacg aaatccaccg cctctcgccg gtcgtcgaag aaatcctcta
     5101 ccccgcgctc gaagacttcc agatcgacat cctcatcggc gaaggccccg ccgcgcgcag
     5161 cgtcaagctc gacctgcagc ccttcaccct ggtcggcgcc accacccgcg ccggcatgct
     5221 caccaacccc ctgcgcgacc gcttcggcat cgtctcgcgc ctggagttct acacggccga
     5281 agaactctcg cgcatcgtca cccgcagcgc gggcctgctc aacgccgcca tcacgcccga
     5341 cggcgcccag gaagtcgccc gccgcgcgcg cggcacgccg cgcatcgcca accggctgct
     5401 gcgccgcgtg cgcgactacg ccgaggtcaa ggccggcggc accatcgaca ccgaggtggc
     5461 cagccgcgcg ctggccatgc tggaagtcga cccgcagggg ctggacctga tggaccgcaa
     5521 gctgctggaa gccatcgtgc acaagttcga cggcgggccg gtaggcgtgg acagcctggc
     5581 cgccgccatc ggcgaagaac gcgacaccat cgagg
//
LOCUS       DASWPO010000218         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_674922, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000218 DASWPO010000000
VERSION     DASWPO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674922"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGC69_11660"
     CDS             complement(<1..473)
                     /locus_tag="VGC69_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0295977.1"
                     /translation="MTAAPRTTPASAKPIRATIWKRIYGRVGAHWKALALAGLTMAGA
                     AATQPTLAILMKPLLDGGFSGAKPYYVWSLPLAVISLVLVRGLCTFCSDYVLSWVANN
                     VLRDLRRDMFDRLLCLPDSDFKRGDTGLLLNRFTVETTIVVNSATEVATAIIREGL"
     gene            543..1511
                     /locus_tag="VGC69_11665"
     CDS             543..1511
                     /locus_tag="VGC69_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0295978.1"
                     /translation="MPAISRLYVRLPNWVGDVCMSLPALHLLQQAGPPLVLCARPWAL
                     DLLAGLPAHDFVALRGKLREDRAAVAAHRRALGREARAARGLLIPDSLSSALVFRLAG
                     LPSAGFRDEGRSMLLRWPIAKPGPALHTVQAWHYLARTALRQWSLPAASDAPGPRLGL
                     PLTAAHREAARALLREQGLDGRPFVLIAPTAVGLHHGRVKVWPHFDALTRALQADGHT
                     VIMCPPPAEAGQARANAPTATLLAPLGLGAYAALAAQARLVVCNDSGVSHIAAAVGAR
                     QLTLFGVTQASRTGPWSPTARGLGSDLAWPEIDAVRQTVDGLLAEG"
     gene            1521..2285
                     /locus_tag="VGC69_11670"
     CDS             1521..2285
                     /locus_tag="VGC69_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY0295979.1"
                     /translation="MEPSSSLANLIIPLNLCLALLVLGVLLRLLRLRRLGALVALLGL
                     AWALAWSLPVTSVWLGGALEQRYPHLAPADAPTAEAIVVLGGNTANGRSNWFLPLDKE
                     TAVARMDVATGLYQAGRAPKIVLSGGALSGEVSEARVLAHRMRQDGIPEEALILENSS
                     RTTYENAALTEDTLELHDIHTILLVTSALHMPRAMAAFAKQGVQATAVPAPPQITLPA
                     DDSVMPWLPNARAFEASRSIIKEYAGLLVYWWRGWV"
     gene            2282..2560
                     /locus_tag="VGC69_11675"
     CDS             2282..2560
                     /locus_tag="VGC69_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0295980.1"
                     /translation="MSIALECRPGCGACCIAPSISSAIPGMPNGKKAGERCVQLDDVN
                     RCRIFGHPDRPQVCGSLMPAAEMCGDSREQALRWLGYVEEMTAPPHEK"
     gene            complement(2567..>3091)
                     /locus_tag="VGC69_11680"
     CDS             complement(2567..>3091)
                     /locus_tag="VGC69_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY0295981.1"
                     /translation="EPQREPQWMCGVLAAVRVMMTRRGKMVFATLDDGSAQVEISIFN
                     ELYEKHRNRLSADQLLVIRGKVSNDDYSGGMRIVAEELYDLQLAREARARSLRVRLNG
                     QADAARLHQMLNPFRAEPENGVPGVPVDIVYRGKAGFECTMRLSEDWRVRMADTLLAN
                     LSEWAKPDGVEVTY"
BASE COUNT          451 a         1069 c         1070 g          501 t
ORIGIN      
        1 agcccctcgc ggatgatggc cgtggcgacc tcggtggccg agttgacgac gatggtggtc
       61 tcgacggtga agcggttgag cagcagcccc gtgtcgccgc gcttgaagtc gctgtcgggc
      121 aggcacagca agcggtcgaa catgtcgcgg cgcaggtccc gcaggacgtt gttggccacc
      181 caggacagca catagtcgct gcagaaggtg cacaagccgc gcaccagcac caggctgatc
      241 acggccagcg gcaggctcca cacatagtac ggtttggcgc cggagaaacc gccgtcgagc
      301 agcggcttca tcaggatggc gagcgtgggc tgggtggccg cggcgccagc catggtcagc
      361 ccggccagcg ccagcgcctt ccagtgggcg ccgacacggc cgtatatccg cttccatatc
      421 gtggcgcgta tgggtttcgc actggcaggc gtggtgcgtg gagcagcagt caaaatgcgg
      481 tctctcaggg gctatcgaca gggtggggat tgtaccgtcc ggccttgcat acaatggcag
      541 ccatgcccgc gatctcccgc ctctatgtcc gcctgcccaa ctgggtgggc gatgtctgca
      601 tgagcctgcc ggccctgcat ctgctgcaac aggcgggccc gcccctggtg ctgtgcgccc
      661 gcccctgggc cctcgacctg cttgccgggc tgcccgcgca cgatttcgtg gccctgcgcg
      721 gcaagctgcg cgaagaccgg gccgccgtgg cggcgcaccg gcgcgcgctg ggccgggaag
      781 cccgggccgc gcgcggcctg ctgataccgg attcgctgtc gagcgccctg gtgttccgcc
      841 tggccggcct gcccagcgcc ggctttcgcg acgaagggcg cagcatgctg ctgcgctggc
      901 ccatcgccaa gcccggtccg gccctgcaca ccgtgcaggc gtggcattac ctggcgcgca
      961 ccgcgctgcg gcaatggagc ctgcccgccg ccagcgacgc gcccggcccc aggctgggcc
     1021 tgccgctgac cgccgcccac cgcgaggcgg cccgggccct gctgcgcgag cagggcctgg
     1081 acggccgccc cttcgtgctg atcgccccca cggccgtcgg gctgcaccat ggccgcgtca
     1141 aggtctggcc gcacttcgat gcgctgacgc gcgcgctgca agccgacgga cacaccgtga
     1201 tcatgtgccc gcctcccgcc gaggccggac aggcgcgcgc caacgcgccg acggccacgc
     1261 tgcttgcgcc gctcgggctg ggcgcctatg cggcgctggc cgcgcaggca cggctggtgg
     1321 tctgcaacga ctcgggggtg tcacatatcg ccgccgccgt gggcgcgcgg caactgaccc
     1381 tgtttggcgt gacccaggcc agccgcaccg gcccctggtc gcccacggcc cgcggcctgg
     1441 gctcggacct ggcatggcct gaaatcgatg ccgtccggca gacggtcgac ggcctgctgg
     1501 ccgaaggcta agattagggc atggaaccat caagttcgct tgccaacctg atcattccgc
     1561 tgaatctctg ccttgccttg ctggtgttgg gagtattgct gcgtttgctc agactgcgcc
     1621 gcctgggcgc cttggtcgcg ctgctggggc tggcctgggc gctggcctgg tccttgcccg
     1681 ttacatccgt ctggctgggc ggcgccctgg aacagcgcta ccctcacctg gccccggccg
     1741 atgcgcccac ggccgaggcc atcgtggtgc tgggcggcaa taccgccaac ggccgcagca
     1801 actggttcct gccgctggac aaggaaaccg ccgtcgcccg catggacgtg gccaccggcc
     1861 tgtaccaggc cgggcgcgcg cccaagatcg tgctgtcggg cggcgccctg tcgggcgaag
     1921 tgagcgaggc cagggtcctc gcccaccgca tgcgccagga cggtattccc gaagaagcgc
     1981 tgattctcga aaacagcagc cgcaccacct atgaaaacgc cgcgctcacc gaggacaccc
     2041 tcgaactgca cgacatccat accattttgc tggtgacttc ggcgctgcac atgccgcgtg
     2101 ccatggcggc ctttgccaag cagggcgtgc aggccaccgc cgtgccggct ccgccgcaga
     2161 tcaccctgcc cgccgacgat tcggtgatgc cctggctacc caatgcacgc gctttcgagg
     2221 ccagccgctc catcatcaag gaatatgcgg gccttctggt gtattggtgg cggggctggg
     2281 tatgagcatc gccctggaat gccggccggg ctgcggagcc tgctgcatcg cgccgtcgat
     2341 ttccagcgcg atccccggga tgccgaacgg caagaaggcg ggcgagcgtt gcgtgcagct
     2401 ggatgacgtc aatcgctgca ggattttcgg ccatccggat cggccgcagg tgtgcgggtc
     2461 gctcatgccc gccgcggaga tgtgcggaga cagccgggag caggcgctgc ggtggctggg
     2521 gtatgtggaa gagatgacgg ctccccccca cgaaaaataa tttccctcag taggtgactt
     2581 cgacgccgtc gggcttggcc cactcggaca ggttggcgag cagggtgtcg gccatgcgca
     2641 cgcgccaatc ctcgctcagc cgcatggtgc actcgaagcc ggccttgccg cggtagacga
     2701 tgtcgaccgg cacgccgggc acgccgttct cgggctcggc gcgaaaggga ttgagcatct
     2761 gatgcaggcg ggcggcgtcg gcctggccat tgaggcgcac gcgcagcgag cgggcgcggg
     2821 cctcgcgggc cagttgcagg tcgtagagtt cctcggccac gatgcgcatg ccgcccgagt
     2881 agtcgtcgtt gctgaccttg ccgcggatca ccagcaactg gtcggcgctg aggcgattgc
     2941 ggtgcttctc gtacagctcg ttgaagatgg atatttcgac ctgggccgag ccgtcgtcca
     3001 gcgtggcgaa caccatcttg ccgcggcggg tcatcatgac gcgcacggcg gccagcacgc
     3061 cgcacatcca ttgcggctcg cgctgcggtt c
//
LOCUS       DASWPO010000219         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1925846, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000219 DASWPO010000000
VERSION     DASWPO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925846"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /gene="secD"
                     /locus_tag="VGC69_11685"
     CDS             <1..1606
                     /gene="secD"
                     /locus_tag="VGC69_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0295982.1"
                     /translation="LGFFEPRPMYLGLDLRGGVHFLLQVDMQGALTTRYDALAGDIRS
                     ALRDQKISVGGVERTDLSVTATFANADDRSKARDALHARMPDLEFTASEDAGKFLLAG
                     ELSPAAVTRVQDAALTQNITTLHNRINELGVSEPIIQQQGSDRIVVELPGVQDVAKAK
                     ELLGRTATLEIRLVDDSPAAAAALNSGTVPFGLERYTDRDGRPILVRRQVILTGENLQ
                     DAQAGRDNQTQQAAVHLTLDDKGARIFRDVTRDNINKRMAILLFENGKGEVVTAPVIR
                     SEIGGGQVQISGSMTAEEAADTALLLRAGSLAAPMSIIEERTIGPSLGADNIAKGFHS
                     TLYGFIAIALFIIVYYHLFGMFSTLGLSLNLLLLLALLSMLQATLTLPGIAAIALTLG
                     MAIDSNVLINERIREELRNGATPQQAIFQGFERAWGTILDSNLTTLIVGLSLLAFGSG
                     PVRGFAVVHCLGILTSMFSSVVGVRAMANFWYGRQRKLAGISIGTVWKPDQADAKSGA
                     AAKPAASNKALAKPAPKAPAKAPAKQ"
     gene            1660..2589
                     /gene="secF"
                     /locus_tag="VGC69_11690"
     CDS             1660..2589
                     /gene="secF"
                     /locus_tag="VGC69_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0295983.1"
                     /translation="MEFFRIHRSIPFMRHALVLNIISLVTFLAAVFFIVTRGFHLSIE
                     FTGGTVMEVNYAQTVQLDEVRGAVSKLGYSDFQVQNFGTSRDVMIRLPLAHGQTSAAQ
                     SDTVLGALKASDASVQLRRVEFVGPQVGRELMHNGLLALLAVVVGIIIYLAFRFEWKF
                     AVSGVLANLHDVVIILGFFAFFQWEFSLSVLAGVLAVLGYSVNESVVIMDRIRENFRK
                     MRKADVREVINSAITQTISRTIITHACTQIMVLSMLFLGGPTLHYFALALTIGIWFGI
                     YSSVFVAAALAMWMGVKREDLIKPVKKDETEVV"
     gene            complement(2590..3150)
                     /locus_tag="VGC69_11695"
     CDS             complement(2590..3150)
                     /locus_tag="VGC69_11695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0295984.1"
                     /translation="MSALDNAVPRMAETPPACAFDSRELRRTLGAFTTGVTVMTTLDA
                     AGNLYGVTANSFSSVSLDPPFILWSQALTSSSYPAFRDCERFVVNILADHQIHVSNQF
                     ARSGGDKFRGIAVREGLGGVPIIDDCAAHLECAKVAAYPGGDHVVYLGQVERIHRGLH
                     RPLAFGDGRYVVPVAHETAQAAAAAR"
     gene            complement(3265..4203)
                     /locus_tag="VGC69_11700"
     CDS             complement(3265..4203)
                     /locus_tag="VGC69_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0295985.1"
                     /translation="MNKLIDPPSLTRLELAELETFLWVAREGSFSVAARKLHLSQPAV
                     TNRVRRLEDKLRVKLLVRTTRRVELTPDGELLRGAAEQAVAGLRDVIGKFQSVSESDR
                     NRVTVVVTPMLAATIMPALIHAYTERYPDVHIVLRDLPYDQVIRSVTEGHADMAVAAL
                     DSAPRGLRFQSLAEEKMVLVVPARHPLAGSSIATLDMILPYPLMLLDRYQTLRKQLQA
                     EFDRRGVPFNPGSTITLPTLLGMVDAGNCITFLPRSMAQDNARHVRKLLEVEDLAVLR
                     CYGTLVVRKAELSAAAQNFQAFLGKNFKTRLARSPE"
     gene            4330..5691
                     /locus_tag="VGC69_11705"
     CDS             4330..5691
                     /locus_tag="VGC69_11705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0295986.1"
                     /translation="MSTPARQMTLIAFLQAQNCTNYAGSWRHPASMTDYLTPEYYQRV
                     ARTLEDGKFDMAFFDDRLAMPDVYGNSHRETVAHGVRPIKLEPTAVLMAMAMVTSRLG
                     LGATYSTTYYEPYHVARLYATLDLMTKGRAAWNVVTSMNNSEAANFGASEHLEHDLRY
                     DRADEFMEVVMGHWDTWQEGAILADKQNDYFADPDKVTRLDHQGRFFNSRGPLTVPRS
                     PQGHPVILQAGQSGRGLAFAARWAEAVFAKYPTLENGKKQYQALKDAVANAGRDPDSV
                     KIAPELKIIVAETESQAREQRDLVASLSRPVDGLTMMGETLNIDFSGRPYEQPFTDEE
                     LAAMSWQSLRDKVVQVSGKRNPSVKDFVEASGRGTLNDGPVFCGTGAQVADQMEEWFK
                     TACDGFVLSGTSVPGTYEDIVRLVVPELQKRGLFRKEYADTTLRGNLGIVKPRAGDWR
                     RNA"
     gene            5706..7070
                     /locus_tag="VGC69_11710"
     CDS             5706..7070
                     /locus_tag="VGC69_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY0295987.1"
                     /translation="MTTKTVRMMSASGILGYGFPESSIKAALELKPHMIGVDGGSSDP
                     GPHYLGSGKTLNSRLAMKRDLSLLLRGAIANGIPMMVGTCGGAGGTPHLQACAEIIRE
                     IAAEHGLHFKMALIDSEQDKGFLLEQLRAGKIKPLNNAPELLAENLEEAVRIVGMMGP
                     EPYRQALAAGAQVILGGRGTDPAPWVALAMHHGLPQAPAWYAGKILECACNAAIPKKH
                     DCLMVTVGEDFVETEPLNPELRCTPMSVAVQALHENASPIIRFEPGGVVDSSDCRIEA
                     LTDRRVRITGMRWTPRPYSIKLEGARMAGYSAITIAATRDPGLIGQLDSFMDFVRAST
                     ATKVSALGIAPESYQLVLRLYGRDGVMGAWEPQPDSTPTEVAIIAEAVAPTQEVANAA
                     LSLARVTLLHSDFPGRMCREGNMAFPFSPSDIERGPIYEFMLQHVLEIDDPLRMFPIH
                     YEQV"
     gene            7077..>7217
                     /locus_tag="VGC69_11715"
     CDS             7077..>7217
                     /locus_tag="VGC69_11715"
                     /inference="COORDINATES: protein motif:HMM:NF025688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 family protein"
                     /protein_id="HEY0295988.1"
                     /translation="MVKLKHIAKACKSKNAGPFHITLDIMFDDPALFERVRGTGVITA
                     ELI"
BASE COUNT         1310 a         2519 c         2250 g         1138 t
ORIGIN      
        1 gctgggcttc ttcgagccgc gccccatgta cctgggcctg gatctgcgcg gcggcgtgca
       61 ctttctgctg caggtcgaca tgcagggcgc gctgaccacc cgctacgacg cgctggccgg
      121 cgacatccgc tcggccctgc gcgaccagaa aatcagcgtg ggcggcgtcg agcgcaccga
      181 cctgtcggtg acggcgactt tcgccaacgc ggacgaccgc agcaaggcgc gcgatgccct
      241 gcacgcccgc atgcccgacc tggaattcac cgccagcgaa gacgcgggca aattcctgct
      301 cgccggcgag ctgagcccgg ccgccgtcac ccgcgtgcag gacgccgccc tcacccagaa
      361 catcaccacc ctgcacaacc ggatcaacga gctcggcgtg tccgagccca tcatccagca
      421 gcagggctcc gaccgcatcg tcgtcgagct gcccggcgtg caggacgtgg ccaaggccaa
      481 ggaactgctg ggccgcaccg ccacgctgga aatccgcctg gtcgacgact cgccggccgc
      541 cgccgccgcg ctcaacagcg gcaccgtgcc cttcggcctg gagcgctaca ccgaccgcga
      601 cggccgtccc atcctggtgc gccgccaggt gatcctcacc ggcgaaaacc tgcaggacgc
      661 ccaggccggc cgcgacaacc agacccagca ggccgccgtg cacctcacgc tcgacgacaa
      721 gggcgcgcgc atcttccgcg acgtcacccg cgacaacatc aacaagcgca tggccatcct
      781 gctgttcgag aacggcaagg gcgaagtcgt caccgccccg gtcatccgca gcgaaatcgg
      841 cggcggccag gtgcagatct cgggcagcat gacggccgaa gaagccgccg acaccgccct
      901 gctgctgcgc gcggggtcgc tcgccgcgcc catgagcatc atcgaggaac gcaccatcgg
      961 ccccagcctg ggcgccgaca acatcgccaa gggctttcac tcgacgctct acggcttcat
     1021 cgcgatcgcc ctcttcatca tcgtctacta ccacctgttc ggcatgttct cgacgctggg
     1081 cctgtcgctc aacctgctgc tgctgctggc gctgctgtcg atgctgcagg ccacgctcac
     1141 cttgcccggc atcgcggcca tcgcactcac gctgggcatg gccatcgact ccaacgtgct
     1201 gatcaacgag cgcatccgcg aagaactgcg caacggcgcc acgccgcagc aggccatctt
     1261 ccagggcttc gagcgcgcct ggggcaccat cctcgactcc aacctcacca ccctgatcgt
     1321 gggcctgtcg ctcctggcct tcggctcggg cccggtgcgc ggctttgccg tggtgcactg
     1381 cctgggcatc ctcacctcga tgttctcgtc cgtggtcggc gtgcgcgcca tggccaactt
     1441 ctggtacggc cgccagcgca agctggccgg catctcgatc ggcacggtct ggaagcccga
     1501 ccaggccgat gccaagtccg gcgcggcggc caagcccgcg gccagtaaca aggccctcgc
     1561 caagccggcg cccaaggcgc ccgccaaagc ccccgccaaa cagtgaccaa cctagattga
     1621 tgcccacgga ggccgcgagg tctcccgctt gagaaaacca tggaattttt ccggattcat
     1681 cgcagcatcc cgttcatgcg ccacgcgctg gtgctgaaca tcatcagcct ggtcactttc
     1741 ctggcggcgg tgttcttcat cgtgacgcgg ggctttcacc tgtccatcga gttcaccggc
     1801 ggcacggtga tggaagtcaa ctacgcgcag accgtccagc tcgacgaggt gcgcggcgcc
     1861 gtgtccaagc tgggctacag cgacttccag gtgcagaact tcggcacctc gcgcgacgtg
     1921 atgatccgcc tgcccctggc ccacggccag acctcggccg cgcagagcga caccgtgctg
     1981 ggcgcgctca aggcgtccga cgcctcggtg cagctgcgcc gcgtcgagtt cgtcggcccg
     2041 caggtgggcc gggagctgat gcacaacggc ctgctggcgc tcctggcggt ggtggtcggc
     2101 atcatcatct acctggcctt ccgcttcgaa tggaagttcg ccgtgtccgg cgtgctggcc
     2161 aacctgcacg acgtggtcat catcctgggc ttcttcgcct tcttccagtg ggagttctcg
     2221 ctgtcggtgc tggccggcgt gctggcggtg ctgggctatt cggtgaacga gtccgtggtg
     2281 atcatggacc ggatccgcga aaacttccgc aagatgcgca aggccgacgt gcgcgaagtg
     2341 atcaacagcg ccatcacgca gaccatctcg cgcaccatca tcacccacgc ctgcacccag
     2401 atcatggtgc tgtccatgct gttcctgggc ggccccacgc tgcactactt cgccctggcg
     2461 ctgaccatcg gcatctggtt cggcatctac tcgtcggtct tcgtggccgc ggcgctggcc
     2521 atgtggatgg gggtcaagcg cgaagacctg atcaagccgg tcaagaagga cgagaccgaa
     2581 gtggtctgat cagcgcgcgg cggccgcggc ctgcgccgtc tcgtgggcca cgggcaccac
     2641 atagcggccg tcgccgaaag ccagcggccg gtgcagcccg cgatggatgc gttcgacctg
     2701 gccgaggtag acgacgtgat cgccgccggg ataggccgcc accttggcgc actccaggtg
     2761 cgcggcgcaa tcgtcgatga tgggcacccc gcccaagccc tcgcgcaccg cgatgccgcg
     2821 gaacttgtcg ccgcccgacc gggcaaactg gttggagaca tggatctggt gatcggccag
     2881 gatgttgacg acgaagcgct cgcaatcccg gaacgccggg tagctgctcg acgtcaacgc
     2941 ctggctccac aggatgaagg gcggatccag cgacaccgaa ctgaaggaat tggcggtcac
     3001 gccataaagg ttgccagcgg cgtccagcgt ggtcatcacc gtgacgcccg tggtgaaagc
     3061 gcccagcgtg cgccgcaatt cccggctgtc gaacgcgcat gccggcggtg tttcagccat
     3121 gcgcggcact gcattgtcca aggctgacat agccttctcc tgttattaga ctgggcacaa
     3181 gtctaggagg ctcccatggc ctgggacaat ttgcggagtt gaattcatct ataaccagcg
     3241 ggaataaaag cccgcgggcc ggcgctactc gggcgagcgc gccaggcgcg tcttgaagtt
     3301 cttgcccagg aatgcctgga aattctgcgc cgcagcactc agctcggcct tgcgcaccac
     3361 cagcgtgccg taacagcgca acacggccag atcctcgacc tcgagaagct tgcggacatg
     3421 ccgggcattg tcctgcgcca tggagcgcgg caaaaaggtg atgcaattgc ccgcatccac
     3481 catgcccagc agcgtcggca gggtgatggt gctgcctgga ttgaacggca cgccgcgccg
     3541 gtcgaactcc gcctgcaact gcttgcgcag ggtctggtag cggtccagga gcatgagcgg
     3601 gtacggcagg atcatgtcca gggtcgcgat gctcgatccc gccagcggat ggcgcgccgg
     3661 aaccaccagc accattttct cttccgccag cgactggaag cgcagcccgc gcggcgcgct
     3721 gtccaaggcc gccacggcca tgtcggcatg gccctcggtc acgctgcgga tcacctgatc
     3781 gtagggcagg tcgcgcaaca cgatgtgcac gtcggggtag cgctcggtgt aggcatggat
     3841 cagggcgggc atgatggtgg cggccagcat gggcgtgacc accacggtga cgcggttgcg
     3901 atcgctctcc gaaaccgact ggaattttcc gatcacatcg cgcaggccgg ccacggcctg
     3961 ctcggccgca ccccgcagca actcgccgtc gggagtgagc tcgacgcggc gggtcgtgcg
     4021 caccagcagc ttgacccgca gcttgtcttc gaggcgccgg acccggttgg tcaccgccgg
     4081 ctgggacaga tgcagtttgc gcgctgcaac actgaagctg ccttcccgcg ccacccagag
     4141 aaaggtctcg agctcggcga gctccaatcg agtcagcgag ggtggatcga tcaacttatt
     4201 catgggcgtt atgaatgtat cacagcgcgg aaattgtgct cagccaagtc tgaaccaata
     4261 ctgcggaacg gttttcgcat gccccgatgc atgccccgca tgaattccta gacacaacag
     4321 agacacgcca tgagcacgcc tgcgcgccaa atgaccttga ttgcctttct ccaggcacag
     4381 aactgcacga actatgcggg ctcatggcgg caccccgcca gcatgaccga ctatctgacg
     4441 ccggaatact accaacgcgt cgcccgcacg ctggaagacg gcaagttcga catggccttc
     4501 ttcgacgacc gcctggccat gcccgacgtc tacggcaaca gccatcggga aaccgtggcc
     4561 catggcgtgc ggcccatcaa gctggaaccg accgccgtgc tcatggccat ggccatggtc
     4621 acctcgcggc tcggcctggg cgcgacctac tccaccacct actacgagcc ctaccacgtg
     4681 gcgcgcctgt acgccacgct cgacctgatg accaaaggcc gcgcggcctg gaacgtcgtc
     4741 acctccatga acaactcgga ggcggccaac ttcggcgcgt ccgagcatct ggagcacgac
     4801 ctgcgctatg accgcgccga cgaattcatg gaggtggtca tgggccactg ggacacctgg
     4861 caggaaggcg ccatcctcgc cgacaagcaa aacgactatt tcgccgatcc cgacaaagtc
     4921 acgcggctgg atcaccaagg gcgctttttc aattcgcgcg gtccgctcac ggtgccgcgc
     4981 tcgccgcaag gccatccggt catcctgcag gccgggcaaa gcgggcgcgg cctggccttc
     5041 gccgcgcgct gggccgaagc cgtgttcgcc aaatatccca cgctggaaaa cggcaagaag
     5101 caataccagg ccctcaagga cgccgtggcc aatgccggac gcgacccgga ctcggtgaag
     5161 atcgcgcccg agctgaagat catcgtggcc gagaccgaaa gccaggcgcg cgaacagcgc
     5221 gacctggtcg ccagcctgtc gcgccccgtc gacggcctga ccatgatggg cgagacgctg
     5281 aacatcgact tctccggacg cccctacgag caacccttca ccgacgagga attggccgcc
     5341 atgtcgtggc agagcctgcg cgacaaggtg gtgcaggtca gcggcaagcg caatccttcg
     5401 gtcaaggatt tcgtcgaagc ctcgggacgc ggcaccttga acgacgggcc ggtcttctgc
     5461 ggcaccggcg cgcaagtggc cgaccagatg gaagagtggt tcaagacggc ctgcgacggc
     5521 ttcgtgctgt ccggcacctc ggtgcccggc acctacgagg acatcgtccg cctggtcgtg
     5581 cccgaactgc aaaaacgcgg cctgttccgc aaggaatacg ccgacaccac gctgcgcggc
     5641 aatctgggca tcgtcaagcc ccgcgccggc gactggcgcc gcaacgcctg agaggagccg
     5701 cgacgatgac caccaagacc gtacgcatga tgtcggccag cggcattcta ggctatggat
     5761 tcccggaaag ctccatcaag gccgcgctgg aactcaagcc gcacatgata ggcgtggacg
     5821 gcggcagcag cgaccccgga ccgcactacc tgggttcggg caagaccctg aactcgcgcc
     5881 tggccatgaa gcgcgacctg tcgctactgc tgcgcggcgc catcgccaac ggcatcccca
     5941 tgatggtggg cacctgcggc ggcgccggcg gcacgccgca cctgcaggcc tgcgccgaga
     6001 tcatccgcga gatcgccgcc gagcacgggc tgcatttcaa gatggcgctc atcgattccg
     6061 aacaggacaa gggcttcctg ctcgagcagt tgcgcgccgg caagatcaag ccgctgaaca
     6121 acgcgccgga actgctcgcc gaaaacctcg aagaagccgt gcgcatcgtc ggcatgatgg
     6181 gcccggagcc ctatcgccag gccctggcgg cgggcgcgca ggtcatcctg ggcggccgcg
     6241 gcaccgaccc ggcgccctgg gtcgcgctgg ccatgcacca cggcctgccg caagcgccgg
     6301 cctggtatgc cggcaagatc ctggaatgcg cctgcaacgc cgccatcccc aagaagcacg
     6361 attgcctgat ggtcacggtg ggcgaggatt tcgtcgagac cgaaccgctc aatcccgaac
     6421 tgcgctgcac gcccatgtcg gtggcggtgc aggccctgca cgagaacgcc agccccatca
     6481 tccgtttcga gcccggcggc gtggtggatt ccagcgattg ccgcatcgaa gccctgaccg
     6541 atcggcgcgt gcgcatcacc ggcatgcgct ggacgccccg gccctacagc atcaagctgg
     6601 aaggcgcgcg catggcgggc tatagcgcca tcaccatcgc cgccacgcgc gacccgggcc
     6661 tgatcggcca gttggacagc ttcatggact tcgtgcgcgc gtccacggcc accaaggtca
     6721 gcgcgctggg catcgcgccc gaatcctacc aattggtgct gcgcctgtat gggcgcgacg
     6781 gcgtgatggg cgcctgggaa ccgcagccgg acagcacgcc caccgaggtg gccatcatcg
     6841 ccgaagccgt ggcgcccacg caggaagtcg ccaacgcggc cttgtcgctg gcgcgcgtga
     6901 ccttgctgca ctccgacttt cccgggcgca tgtgccgcga aggcaatatg gcctttccgt
     6961 tctcgccttc ggacatcgag cgcggaccga tctacgaatt catgctgcag cacgtcctgg
     7021 agatcgacga cccgctgcgc atgttcccca tccattacga acaggtctga atcgccatgg
     7081 tcaagctcaa gcatatcgcc aaggcctgca aaagcaagaa cgccgggccg ttccacatca
     7141 cgctggacat catgttcgac gaccccgccc tgttcgagcg ggtgcgcggc accggcgtga
     7201 tcacggcgga actgatc
//
LOCUS       DASWPO010000220        20015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_943523, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000220 DASWPO010000000
VERSION     DASWPO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20015
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943523"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VGC69_11720"
     CDS             complement(<1..1240)
                     /locus_tag="VGC69_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0295989.1"
                     /translation="MDRQQIDKLVRADEGLVSRSIFVDDTVFKLEMERIFSRAWLFVG
                     HESLVPKPDDYFVSRMGGESVILTRDRQGQIQVLLNSCTHRGMKLCRYDQGNNRTFTC
                     PYHGWSFSTDGKLAEQPGALVGVPGHATHYKGELDKAQWGLKRVAQLCNYKGTIWATW
                     DPGAPPFLDYLGNMRLYLDAALDHRDGSPGGSEVIGGVQKWRIQCNWKFPPENFIGDM
                     YHDISHRSVDIVGIGPSGGKGRRDQATTRTTICFPELGHGVIGRLPFYEEPPYAPSWT
                     QYPEVEAYFKEIHEKRVQGLGNSMRVQMSVGTIFPNMSFHGRQPRTLAVFHPISATEV
                     EMWRMHLVDKDAPESVKDAARHYFLRYSGPGGMTESDDLENWTGATEASMGPMSRTLD
                     FNYQMGVGHARPVPEIAGCVE"
     gene            complement(1308..2060)
                     /locus_tag="VGC69_11725"
     CDS             complement(1308..2060)
                     /locus_tag="VGC69_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY0295990.1"
                     /translation="MSFLSSSALLDNADALRSEPNLTDRVAGILLDEVTGGRYQIGDV
                     LPPEQAMATQLGVSRTVLREAVSRLKADGFVQSKQGRGLTVMQTSRPSVLRMYAADDS
                     DVEQVLHIVELRRAFEAEAAMLAAQRRDADDLAALGASLQAMARALASGDVAAGTAAD
                     VEFHRRIARATHNEYYLSFFDFLSLLLKKNLQISRTKSAQTGRGDRAQKEHEMIYAAI
                     EQGDAVLARQCARAHIENTEARLRSERAAGGS"
     gene            complement(2195..3634)
                     /gene="rpoN"
                     /locus_tag="VGC69_11730"
     CDS             complement(2195..3634)
                     /gene="rpoN"
                     /locus_tag="VGC69_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY0295991.1"
                     /translation="MTNRPALELRPGQHLTLTPQLQQSIRLLQLSSIELEMEISQALA
                     ENPLLERDDDPAPAAEAADPQREASVDQDGPSMETAELHFEEMPGSGGVYPDEDGDLP
                     QTAYTDTLREHLLGQLTLTRAEPRDLALVQVLIDELDDNGYLSSPLEEILTWLPAELE
                     VDLDELHAALTLLRSFDPPGIGARDMADCLLLQLRNPDTTHLPEALDLDVLACARLIC
                     VHHLPLLAAGNPARLRDVLQCDAATLRAAYALIVRLDPRPGRAWTVPAADYAVPDVLV
                     RKGRQGWQAVLNSAVVPRLHINGVYAQMLGNQREASHAGLQAQLQQARWMIRNVEQRF
                     DTILRVSQAIVVRQSDFFTRGAAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFG
                     TMELKRFFGTGLATDTGDSASATAVQEHIRQMVAEENRSKPLSDSQIMERLCQRGIVI
                     ARRTVAKYREALRIAPAAVRKAQATVSAR"
     gene            3679..4299
                     /locus_tag="VGC69_11735"
     CDS             3679..4299
                     /locus_tag="VGC69_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0295992.1"
                     /translation="MPNTMQATPDRIRQDRRLIGQALFKLALPRLAILAVGIVIAVAV
                     WLYATNWLLDLGPRVGLDWLHILGPQGAEFLGHLINPYLWWVLVAIWTIIVLYVLKSW
                     IGASLAASRVYTLDSGVLAQLRPRLCDEVVDVLRWVWSDRNEPFTVCDLQRAHQELRS
                     NRIGKIALVRVQSAILDQPAGGDSATPEDRARPARAPAEPWLDRAR"
     gene            4734..6446
                     /gene="leuA"
                     /locus_tag="VGC69_11740"
     CDS             4734..6446
                     /gene="leuA"
                     /locus_tag="VGC69_11740"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY0295993.1"
                     /translation="MMLANPATKYIPFRPFERDFSERTWPSKRITHPPIWMSTDLRDG
                     NQSLIEPMSVERKLRFFEQLVKIGFKEIEVGFPSASQTDFDFVRRLIDEKRIPDDVTI
                     IVLTQSREELISRTVESAAGAKRAMVHLYNATAPAFRKIVFNMTPEEVKNLAIEGTRL
                     IMKHMARHPETQWRYEYSPEVFSTTEPEIALMVSDAVAETWGATPDNKLVLNLPATVE
                     ATQPNLYADQIEWMCKHLARRDSIIVSLHPHNDRGTAVAAAELGVMAGADRIEGCLFG
                     SGERTGNVDLVTLALNLYTQGIHPGLDFSDIDEVRRTAEYCNQLPVHPRHPYAGDLVF
                     TAFSGSHQDAIKKGFAQQKPDAVWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGVSYL
                     IEQEHGLVLPRRLQIEFSRAIQRVTDETGREVTSADVYGLFEREYLSQKSPWALVNHR
                     IETNASAQEGQRYTVTAELEHDGRRRTLAGSGDGALSAFVDAIGVPIRIMDYTEHAIG
                     TGTHTRAASYVEMRIGDAPTGFGVGIDHDIVTASFRAVLSAVNRHAAARGKAETREAD
                     EATA"
     gene            6519..6995
                     /locus_tag="VGC69_11745"
     CDS             6519..6995
                     /locus_tag="VGC69_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0295994.1"
                     /translation="MAHEHKYRVSVQWTGNRGTGTSGYRDYGRDHVIRAQGKPDIAGS
                     SDPAFRGDAARWNPEDLLVASASACHKLWYLHLCSDAGIVVLDYADEAEGTMTDASEP
                     GRFTEIVLRPRVTIRAQDDSAKARHLHHAAHEKCYVANSVNFPIRCEPIIEQAPAP"
     gene            complement(7149..8582)
                     /locus_tag="VGC69_11750"
     CDS             complement(7149..8582)
                     /locus_tag="VGC69_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0295995.1"
                     /translation="MRQHFIDNRACAGASGEQIPVIDPSTGEEYDGIARGDAVDIDLA
                     VQAARRAYDGAWGRLSPAERSRLLLKLAVKVQDHHEELAQIESRDCGKPTKQARADAT
                     ALARYFEFYGGAADKLHGETIPYSQGYTVLTLREPHGVTGHIVPWNYPMQIFGRSVGG
                     ALAAGNACVVKPAEDACLSLLRVAELAAEAGFPPGALNIVTGYGHEAGDLLVRHAGVD
                     HISFTGSPRVGVLVTQAAAERHVPVTLELGGKSPQIVFADADMDAMVPVVLNAIVQNA
                     GQTCSAGSRLLIERGAYEPVLDRVGKAFAELRTGPAAMDLDCGPLIRKTQQERVQGFL
                     RDAERDAIATVARGVVVAEAPTGGFYQAPTLLRDVPVTHRLAQEEVFGPVLSAMPFDD
                     EADAVRIANATEYGLVASLWTRDISRALRLSRRVRSGQVFINNYGAGGGIELPFGGVK
                     ASGHGREKGFEALYGFTVLKTVSIKHD"
     gene            complement(8615..9391)
                     /locus_tag="VGC69_11755"
     CDS             complement(8615..9391)
                     /locus_tag="VGC69_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0295996.1"
                     /translation="MTLPYLRQFSLVDRVALVTGSARGLGLCIARALAGSGAHVLLNG
                     RDAQAVEQAAAALRADGLAASALPFDVSDDAAAEAAFARIDNEFGRLDILVNNVGARN
                     RKTLRAFTPAEVRELIEMDLVAGILLSKRAAERMVRHGYGRLIAITSVVGEIARVGDA
                     VYPAAKQGLTGMMRAVAMEYAPLGVTSNAIAPGTFATETNAAMVADAAVDAAVRGRNP
                     MGRWGLPEEIAGAAVFLASPAASYVNGHVLVVDGGLSVQF"
     gene            complement(9410..10285)
                     /locus_tag="VGC69_11760"
     CDS             complement(9410..10285)
                     /locus_tag="VGC69_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0295997.1"
                     /translation="MSDQQAAALSELTAAALKDQLLQGRRAAVRRRLRISAWQLLILA
                     LILGAWEGLTRIPWFVQNTLFDPFFISQPSRVAVRLWQWMQPGPHSVWPHLLLTLQAT
                     LLGLLTGVGSGFAVGLALSRSRTASEVCNPFIVAFNSMPRIAFVPLITMFFGLGLASK
                     VVTSWFVVFFLVFFNTYKGGRSVERELVDFCRTLGASPRQILWRVRIPTAAAWTFAAL
                     PNAISFALIGVVLAEFVGSTTGMGYLMITALATLNATDMFAAVALLSAVGIILVYAVT
                     WLERRLLHWAPEFRD"
     gene            complement(10303..11073)
                     /locus_tag="VGC69_11765"
     CDS             complement(10303..11073)
                     /locus_tag="VGC69_11765"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0295998.1"
                     /translation="MIELDGVGKAYQGRNGVTHAVGRVDLRIEAGEFVSIVGPSGCGK
                     STLLNMIAGFLPTTTGSVRVEGRPVRDEVPPALGYIFQKDTLLPWFTVRKNVALGLRF
                     HGAPEQDIAPRVESLLALGNLAGFGDAYPHQLSGGMRRRVALLMSLAVDPRILLLDEP
                     FGALDTHTKTHLHRELMDLWRKLGQTIVMVTHDLDEAVTLSTRVVVLSGPPSQVLRDE
                     RIDIPYPRDVFTLRETPQFTTHLQGLWSVLGRQFRAAA"
     gene            complement(11085..12152)
                     /locus_tag="VGC69_11770"
     CDS             complement(11085..12152)
                     /locus_tag="VGC69_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0295999.1"
                     /translation="MNTDYQSMPGAASRRRFLAASTGLAAAGLFGPGRARAQAPVKIV
                     YGQGSIDPFFTAGYLALKKGYFTDNGLEVEYLNSQSGPRTNQLLAAGQILFGATAATA
                     APALTLAGKPAALIFGFDRKLTYASIIVRREDYESGKIRTLKDLAGKRLGATQPQSVT
                     WLMGVYLMQKAGLGDTVDIRPLGDLATMLGALKTGSVSASMATMSMMEQARQEGWGVP
                     IFDATTESAWNELMGGDVPGIAALTLRETIDKRPEVVQGFVSALVKAQDFISAHGAAE
                     VTDAIYDDYLSGFARPAIEKTIAVYQKTVFLKDNIITEDAYGRMTAIMGDGRQFGNDE
                     LAKAPYSACVDMRFVRHARGA"
     gene            complement(12276..14897)
                     /locus_tag="VGC69_11775"
     CDS             complement(12276..14897)
                     /locus_tag="VGC69_11775"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY0296000.1"
                     /translation="MTTKTLIIAEKPSVALDISRALGGFTREGDYFENDQYVLSSSVG
                     HLLGLVAPNDPVKGKWSFTHLPVIPPEFDLGPADKRSTERLKLLVKLAKRKDVDTIIN
                     ACDAGREGELIFRYIIQYAGVKKAIKRLWLQSMTQAAIRDAFLNLRDDTQMKPLEAAA
                     RSRAEADWLVGINGTRAMTAFNSKDGGFFKTPVGRVQTPTLAIVTEREERIRRFVPRD
                     YWEVHGTFVAAAGLYEGRWIDPDFKKDDRDPERRESRLWSQAAAQSVVAACRGQTGSV
                     REESKPSTQISPALYDLTSLQREANGRFGFSAKTTLALAQTLYERHKALTYPRTDSRY
                     LPEDYVATVQQTMQALAQGDSRAVGGLSQHAATVVKNQWVKPNRRIFDNKKVSDHFAI
                     IPTLQVPRELSEAEAKLYDLVLKRFLAVFFPAAEYRVTTRITEVQGHQFKTEGKILVA
                     PGWLAVYGKEAQGEDALLTLVAEGEKVRTEEVEAVGLTTKPPARYNEATLLSAMEGAG
                     KLVDDEELREAMSERGLGTPATRASIIEGLINETYLRREGRDLVPTAKSRQLMTLLSG
                     LDVSELTSPELTGEWEHKLKQIEQGSLNREAFMREIAQMTQVIVKRAKEYERDTVPGD
                     YATLQTPCPKCGGVVKENYRRYACTNCDFSISKHPGGRTFELPEVEDLLAKREIGPLQ
                     GFISKMGRPFAAILRISDEYKLEFDFGQSDDDDAQAVDFSGHTPVGICPKCGSKVFEH
                     GMSYVCEKSVGPEKSCDFRSGKVILQQEISRAQMEKLLADGRTDLLDGFVSSRTNRKF
                     KAFLVRQPDGKVGFEFEPRPERPARKTAAKTAAKTASAQTPVAVKKAAAKTAVKKTAA
                     KTAARKAVKKAAKKTAG"
     gene            complement(14988..15860)
                     /gene="gluQRS"
                     /locus_tag="VGC69_11780"
     CDS             complement(14988..15860)
                     /gene="gluQRS"
                     /locus_tag="VGC69_11780"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY0296001.1"
                     /translation="MYIGRFAPSPSGPLHAGSLAAALASWLDARARAGRWLVRIEDVD
                     TPRTVAGADALILSQLRALGLRCDGDVVWQSKRGALYQRAFDGLAARGLVYGCGCTRR
                     EIADSQPDGALGADGERPYPGTCRHGLAPGRQARAWRVRVPPGVERFDDRWLGPQQQD
                     VAQAVGDFALRRADGLWAYQLAVVVDDAEQAVTDVVRGADLLGSTARQRVLGRMLDLP
                     LPRVMHVPLVVDPATGLKLSKQNHAQALDVGRPLAALDAAWRKLGFASLPARDVPVFL
                     AEATAQWGRRFPLT"
     gene            complement(15976..16434)
                     /locus_tag="VGC69_11785"
     CDS             complement(15976..16434)
                     /locus_tag="VGC69_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY0296002.1"
                     /translation="MFDILVYLFENYYTPQACPTADVLAKRLAAAGFEHEDIDDALGW
                     LYGLAETTERCVELAQVQSEGVRIYADSEYRQLGTESIGFITFLESAGILPAPLREIV
                     IDRALAAPEAPVPLSCTKIIALMVLWSQEAEIDNLVLDELLDDEGGRRLH"
     gene            complement(16587..17603)
                     /locus_tag="VGC69_11790"
     CDS             complement(16587..17603)
                     /locus_tag="VGC69_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0296003.1"
                     /translation="MTEAPSQNVAELSARIDRVTSVKIQEIKSITLETRLLSLNAAIE
                     AAHVGEAGRSFAVVAGRVREVSDRVEALANSLQQELSEVGGSMHQALYRSRGQRLADM
                     ALNVIETIDRNLYERTCDVRWWATEAAVQALAAEPDDDDLAAQTRQRLGVILGAYTVY
                     LDIWVVDPSGRVLCNGRPDAYPAARGHDVSGQDWFRQALAGRGLDAYSAGDVQPVAAL
                     GGARSAIYAAAIHADAECLDTPRGVLAVFFDWGKQASAVVNGIRVSAEEAESVRCMVV
                     DARRHVIAANNSDVPATIDLPADRMDGFYTKDTDLIGYARTPGYETYQGLGWYGVVIE
                     RGAV"
     gene            17905..19095
                     /locus_tag="VGC69_11795"
     CDS             17905..19095
                     /locus_tag="VGC69_11795"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEY0296004.1"
                     /translation="MQFSQFNVNALMEITSRPDLVFVRGEGSWLEDHTGKRYLDFIQG
                     WAVNTLGHCAPEMRQAIIEQSGKLMNPSPAFYNLPSIELAERLAKSSVFKRVFFANSG
                     GEANEGAIKLARKWGKVKKNGAYKIITMDHGFHGRTLATMSASGKPGWDTMFAPQVPG
                     FPKADLNDLDSVKKLIDLDTVAIMLEPVQGEAGVYPATQAFMQGLRKLADENQLLLIV
                     DEVQTGMGRTGKLFAYQHFGITPDIMTLAKGIGGGVPLAALLAREEVSVFSHGDQGGT
                     YNGNPLMTAVGVAVFDKLASPGFMESVNTRARQLSEGLLALSAKWGFEGERGLGLLRA
                     LILDADIGPAIVDAARDLAPQGLLLNAPRANLLRFMPALNVSEAEVAQMLEMLDGVIA
                     KLKK"
     gene            19272..19604
                     /locus_tag="VGC69_11800"
     CDS             19272..19604
                     /locus_tag="VGC69_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0296005.1"
                     /translation="MPYTISYYSDAFALEILALPPTLLAHYLNLTDRMVHAGPNLGPP
                     HTEAFGNGLFQLRLKRAEGISRVFYCTLAGQRIVMLHGFIKKTKKTPPAERRIAEARL
                     KEVKRDHT"
     gene            19591..19878
                     /locus_tag="VGC69_11805"
     CDS             19591..19878
                     /locus_tag="VGC69_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0296006.1"
                     /translation="MITHEKLRARALANPAVRAEYERIEREEMPMLDAILKARAEAGL
                     TQAQVAERMGTKAPAVARLENALITGAPSPSLNTLRKYAAALGKRLEIRLV"
     gene            complement(19875..>20015)
                     /locus_tag="VGC69_11810"
     CDS             complement(19875..>20015)
                     /locus_tag="VGC69_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY0296007.1"
                     /translation="NSQHRADAMKSWLHHYNWHRPHQGIGRAVPISRLNLDGYNLLTL
                     HS"
BASE COUNT         3356 a         6870 c         6502 g         3287 t
ORIGIN      
        1 tctcgacgca acccgcgatc tcgggaaccg gccgggcgtg gcccaccccc atctggtagt
       61 tgaaatcgag cgtgcgcgac atcggcccca tgctggcttc ggttgcgcca gtccagtttt
      121 ccaggtcgtc ggactcggtc atgcccccgg gcccggaata gcgcaggaaa taatggcgcg
      181 ccgcgtcttt caccgactcc ggcgcgtcct tgtccaccag gtgcatgcgc cacatttcca
      241 cttcggtggc gctgataggg tggaatacgg ccagggtgcg gggctggcgg ccatggaagg
      301 acatattggg aaagatggtg cccacgctca tctgcacgcg catggaattg cccaggccct
      361 gcacgcgctt ttcatggatc tccttgaaat acgcctccac ctcgggatat tgggtccacg
      421 aaggcgcgta gggcggctcc tcgtagaacg gcaggcggcc gatcacgcca tgccccagct
      481 cgggaaagca gatggtcgtg cgcgtggtgg cctggtcgcg ccgccccttg ccgccgctgg
      541 ggcctatgcc cacgatatcg accgaacgat ggctgatgtc gtgatacatg tcgccgatga
      601 agttctccgg cgggaatttc cagttgcact ggatgcgcca cttctgcacg ccgccgatga
      661 cctccgagcc gcccgggctg ccgtcgcgat ggtccagcgc ggcatccaga tacaggcgca
      721 tattgcccag gtaatcgaga aaaggcggcg cgccgggatc ccaggtagcc cagatggtgc
      781 ctttgtagtt gcagagttgg gccacccgct tgagccccca ctgcgccttg tcgagctcgc
      841 ccttgtaatg cgtggcatgc cccggcacgc ccaccagcgc gccgggctgt tccgccagct
      901 tgccgtccgt ggaaaaactc cagccgtggt agggacaggt gaaggtgcga ttgttgccct
      961 ggtcatagcg gcacaacttc atgccgcgat gcgtgcagct gttgagcagc acctggatct
     1021 ggccctggcg atcgcgagtc aggatgaccg actcgccgcc catgcgcgat acgaaatagt
     1081 cgtccggctt gggcaccagg ctctcgtgcc cgacaaacag ccaggcccgc gagaaaatcc
     1141 gctccatctc gagcttgaaa acggtgtcgt cgacgaaaat cgaacggctc accaggcctt
     1201 catcggccct gaccagttta tctatctgct gacggtccat gtgttttccc cttgcggttc
     1261 cgtccggtat tcaaatcggt atgtatccac tgcggccgag acggggctca gctaccgcca
     1321 gcggcccgct cgctgcgcag gcgggcctcc gtattctcga tatgggcgcg cgcgcattgg
     1381 cgagccagga cggcatcgcc ctgctcgatg gcggcgtaga tcatttcgtg ctctttctgc
     1441 gcccggtcgc cgcggccggt ctgcgccgac ttggtgcgtg agatctgcag gttcttcttg
     1501 agcagcagcg aaagaaaatc gaagaaggag aggtaatact cgttgtgcgt ggcgcgcgcg
     1561 atccggcggt ggaactccac atcggcggcc gtcccggcgg cgacgtcgcc gctggccagg
     1621 gcccgcgcca tggcctgcag gctggcgccc aatgcggcca gatcgtccgc gtcgcggcgc
     1681 tgcgcggcca gcatggcggc ctccgcctcg aaagcccgcc gcagctcgac gatgtgcagc
     1741 acttgctcga catcgctgtc atcggcggca tacatgcgca ggaccgaagg gcggctggtc
     1801 tgcatgacgg tcaggccgcg gccctgcttg ctttgcacga acccatccgc cttcagcctc
     1861 gacacggcct cccgcagcac cgtgcgggac acgcccagct gggtggccat ggcttgctcc
     1921 gggggaagca cgtcgccgat ctggtagcgc ccgccggtca cttcatcgag caggatgccc
     1981 gcgacccgat ccgtcagatt gggttcggac cggagcgcat cggcgttgtc gagcagggcg
     2041 gaagacgaaa gaaacgacat gggtacggat tcaaatacca tttgataggt acgataatca
     2101 tacaaagtcg tcatgcctac tagggaaagc gcgagcactg tcccccgcgg acggcaggca
     2161 aagggaaaac gggaattagg aaacgggggc caagtcagcg cgccgacacc gtcgcctgcg
     2221 ccttgcgcac cgcggccggg gcgatgcgca gcgcctcgcg gtacttggcc accgtgcggc
     2281 gggcaatgac gatgccgcgc tggcacaggc gctccatgat ctggctgtcg gacaggggct
     2341 tgctgcggtt ttcctcggcc accatttggc ggatgtgctc ctgcacggcc gtggccgagg
     2401 ccgagtcgcc ggtatcggtg gccagcccgg tgccgaagaa gcgcttgagc tccatcgtgc
     2461 cgaacggtgt gagcatgtac ttctgcgttg tggcccgtga aatcgtggat tcgtgcaagc
     2521 ccagctcgcc ggcgatgtcc ttaaggatca ggggccgcat ggcggccgcg ccgcgggtga
     2581 agaagtccga ctggcgcacc acgatggcct gcgacacgcg caggatggtg tcgaaacgct
     2641 gctcgacgtt gcggatcatc cagcgggcct gctgcagctg cgcctgcagg ccggcatggc
     2701 tggcctcgcg ctggttgccc agcatctggg cgtagacccc gttgatgtgc aggcgcggca
     2761 ccacggcgct gttgagcacg gcctgccagc cctggcggcc cttgcgcacc agcacgtcgg
     2821 gcacggcata gtcggccgcc ggcacggtcc aggcgcgccc cgggcgcggg tccagccgca
     2881 cgatcagggc ataggccgcg cgcagcgtgg ccgcgtcgca ctgcagcacg tcgcgcagcc
     2941 gggccgggtt gcccgccgcc agcaggggca ggtgatggac gcagatcagc cgcgcgcagg
     3001 ccagcacgtc caggtcgagc gcttccggca ggtgggtggt gtcggggttg cgcaattgca
     3061 acaacaggca gtcggccatg tcgcgcgcgc cgatgccggg gggatcgaac gaccgcagca
     3121 gcgtcagcgc ggcgtgcagt tcgtcgaggt cgacttccag ttcggccggc agccaggtca
     3181 ggatttcctc gagcggggaa ctcaggtagc cgttgtcgtc gagctcgtcg atcagcacct
     3241 gcaccagcgc caggtcgcgc ggctcggcgc gggtcagcgt cagctggccc agcaggtgtt
     3301 cacgcagcgt gtcggtgtac gccgtctgcg gcaagtcgcc gtcttcgtcg gggtacaccc
     3361 cgcctgaacc cggcatttcc tcgaaatgca gctcggccgt ctccatcgac ggaccgtcct
     3421 ggtcgaccga ggcctcgcgc tgcgggtcgg ccgcctcggc cgccggggcc ggatcatcgt
     3481 cgcgttcgag caaagggttt tcagccaacg cctgcgagat ctccatttcc agttcgatcg
     3541 acgacagctg cagcaaccgg atggactgct gcagctgcgg cgtcaaggtc aggtgctggc
     3601 ctgggcggag ttctagggca ggacgattgg tcataggtac aatcataatg gcgcattagc
     3661 gcgccgcaga ccatttgagt gccaaacacg atgcaagcca cgcccgaccg tatccgccag
     3721 gaccgccgcc tcatcggcca ggccctgttc aagctggcgc tgccgcgcct cgccatcctg
     3781 gccgtaggca tcgtcattgc cgtggcggtc tggctgtatg ccacgaactg gctgctggac
     3841 ctgggtccgc gggtcgggct cgactggctg cacatcctgg gtccgcaggg cgccgagttc
     3901 ctcggccacc tcatcaatcc ctacctctgg tgggtcctgg tggccatctg gacgatcatc
     3961 gtcctctacg tcctgaagtc ctggatcggc gccagcctgg ccgccagccg cgtctatacc
     4021 ctcgacagcg gcgtgctggc ccagctgcgc ccccgcctgt gcgacgaggt ggtcgacgtg
     4081 ctgcgctggg tctggagcga ccgcaacgaa cccttcacgg tgtgcgacct gcaacgcgcc
     4141 caccaggaat tgcgcagcaa ccgcatcggc aagatcgccc tggtgcgggt gcaaagcgcc
     4201 attctcgacc agccggccgg cggcgactcg gccacgcccg aagaccgggc gcgtcccgcg
     4261 cgcgcgccgg ccgaaccttg gctcgaccgg gcgcgctgac gcccggcccc ggcccgaatt
     4321 tgcatcggcc cggaaagtat ggaagacttc agggcatgga cgcgcaacgc accttcttcg
     4381 cacacgccgc tacggccacg ggcaacgccc gcgccgccgc gtttgcgtct gcctttgcgc
     4441 ccgtcttttc gtccattgcc gccgcggccc ccgcgcgcct tccctcggcc acctcggccc
     4501 tagccctacc cctaagccta gcgatcggtt gccgggccta gtgcctcatg tggtagctcg
     4561 gcagccctca ctgtagccat catcggcttc agtacgcttg ccacaggctt gctcccgttt
     4621 taaaaacaga atccagcccg cgccatcctt gcgggcctgc gccggtacaa cccgggccgc
     4681 taccctggcc cgccgccggc ccgacggatt cacaagaccc gaggtgacct gacatgatgc
     4741 ttgccaaccc cgctaccaaa tacatcccct tccggccgtt cgagcgcgat ttttccgagc
     4801 gcacctggcc tagcaaacgc atcacccatc cgcccatctg gatgagcacc gacctgcgcg
     4861 acggcaacca gtcgctgatc gaacccatga gcgtggagcg caagctgcgc ttcttcgagc
     4921 agctggtgaa gatcggcttc aaggagatcg aggtgggctt tccctcggcc tcgcagaccg
     4981 acttcgattt cgtgcgccgg ctgatcgatg aaaaacgcat ccccgacgac gtcaccatca
     5041 tcgtgctgac gcaatcgcgc gaagagctga tcagccgcac ggtggagtcc gccgccggcg
     5101 ccaagcgcgc catggtgcac ctgtacaacg ccaccgcgcc ggcctttcgc aagatcgtct
     5161 tcaacatgac gccggaagaa gtgaagaacc tcgccatcga gggcacccga ctgatcatga
     5221 agcacatggc ccgccacccc gagacgcaat ggcgctacga atactcgccc gaggtcttct
     5281 ccaccaccga gcccgagatc gcgctgatgg tctccgatgc cgtggccgag acctggggcg
     5341 ccacgcccga caacaagctg gtgctgaacc tgcccgccac cgtcgaggcc acccagccca
     5401 acctgtacgc cgaccagatc gagtggatgt gcaagcacct ggcgcgccgc gacagcatca
     5461 tcgtcagcct gcacccgcac aacgaccgcg gcaccgccgt ggcggccgcc gagctcggcg
     5521 tgatggccgg cgccgaccgc atcgaaggct gcctcttcgg cagcggcgag cgcaccggca
     5581 acgtcgacct ggtcacgctg gcgctcaacc tctacacgca gggcatccac cccggcctgg
     5641 atttctccga catcgacgaa gtgcgccgca cggccgagta ctgcaaccag ctgcccgtgc
     5701 atccgcgcca cccctacgcg ggcgacctgg tgttcaccgc cttctccggc tcgcaccagg
     5761 acgccatcaa gaaaggcttc gcgcagcaaa agcccgatgc cgtgtgggaa gtgccctacc
     5821 tgcccatcga tccagccgat ctcggccgca gctacgacgc ggtgatccgc gtcaacagcc
     5881 agtcgggcaa gggcggggtg tcgtacctga tcgagcagga acacggcctg gtgctgccgc
     5941 gccgcctgca gatcgagttt tcccgcgcca tccagcgcgt gaccgacgaa accggccgcg
     6001 aagtcacctc ggccgacgtg tacggcctct tcgagcgcga atacctgtcg cagaagtcgc
     6061 cctgggcgct ggtgaaccac cgcatcgaaa ccaacgcctc ggcccaggaa ggccagcgct
     6121 acaccgtcac cgccgagctc gagcacgacg gccgccgccg caccctcgcc ggcagcggcg
     6181 acggcgcgct ttccgccttc gtcgacgcca tcggcgtgcc catccgcatc atggactaca
     6241 ccgagcacgc catcggcacc ggcacgcaca cccgcgcggc cagctacgtc gagatgcgca
     6301 tcggcgacgc gcccacgggc ttcggcgtgg gcatcgacca cgacatcgtc acggcttcgt
     6361 tccgcgccgt gctgagcgcg gtgaaccggc atgcggcggc gcgcggcaag gccgaaacca
     6421 gggaagccga cgaagcgacg gcctgagccg cccgccggtc catgcgcgtg ggtaaactgt
     6481 gatgcacgcg gcacgcgccg ccctcacagg agcccgccgt ggcgcatgaa cacaaatacc
     6541 gagtctcggt gcaatggacc ggcaaccgcg gtaccggcac ctccggctac cgcgactacg
     6601 gccgggatca cgtgatacgg gcgcagggca agccggatat cgccggttcg tccgacccgg
     6661 cgtttcgcgg cgacgccgcg cgctggaacc ccgaagacct gctggtggcc tcggcctcgg
     6721 cctgccacaa gctctggtat ctgcacctgt gctccgacgc gggcattgtc gtgctggatt
     6781 atgcggacga ggccgaaggc acgatgaccg acgcatcgga gcctggcagg ttcaccgaga
     6841 tcgtgctgcg tccacgcgtc accatccgcg cgcaggacga ctcggccaag gcacggcact
     6901 tgcatcatgc cgcccacgaa aaatgctatg tcgccaattc agtgaacttc cccatacgct
     6961 gcgagccgat catcgaacag gctcctgcgc cctgagtcgc cctgggcgtg ctgcagcgcc
     7021 tctcgttctg gatattcacg cgcggatcgc caggccgcgg ctttcgatca ttcattcatg
     7081 ccgtaataaa ccacgccatc tgcgcaggat ggcatgcagc gccttccggc caaggcgccg
     7141 tatggatgtc aatcgtgctt gatcgagacg gtcttgagca cggtgaagcc atacaaggcc
     7201 tcgaagccct tttcgcggcc gtggccgctg gccttcacgc cgccgaaggg cagttcgatg
     7261 ccgccgcccg cgccgtagtt gttgatgaag acctggccgc tgcgcaccct gcgggacaac
     7321 cgcagggcgc gcgagatgtc gcgcgtccag aggctggcga ccaggccgta ttcggtggcg
     7381 ttggcgatgc gcacggcatc ggcctcgtcg tcgaagggca tggccgacaa caccgggccg
     7441 aagacttctt cctgcgccag gcggtgcgtg accggcacat cgcgcaatag cgtgggcgcc
     7501 tggtagaaac cgccggttgg cgcctcggcc accacgacgc cgcgcgccac ggtggcgata
     7561 gcgtcgcgct cggcgtcgcg cagaaagccc tgcacgcgct cctgctgggt cttgcgaatc
     7621 agcgggccgc agtccaggtc catggcggcc gggccggtac gcaactcggc aaacgccttg
     7681 ccgacccggt cgagcaccgg ttcgtaggcg ccgcgctcga tcagcaagcg gctgccggcc
     7741 gagcaggtct gcccggcgtt ctgcacgatg gcgttgagca ccaccggcac catggcgtcc
     7801 atgtcggcat cggcgaacac gatctgcggc gacttgccgc ccagttcgag ggtgaccggc
     7861 acgtgccgct cggccgcggc ctgcgtcacc agcacgccca cgcgcggcga gccggtgaac
     7921 gaaatgtggt ccaccccggc gtggcgcacc agcaggtcgc ccgcttcgtg gccgtagccc
     7981 gtgacgatgt tcaacgcccc cggcggaaag ccggcctccg cggccagttc ggccacgcgc
     8041 agcagggaca ggcaggcgtc ctcggccggc ttgaccacgc aggcattgcc cgccgccagc
     8101 gcgccgccca cgctgcggcc gaagatctgc atggggtagt tccagggcac gatatggccg
     8161 gtgacgccgt gcggctcgcg caaggtcagc acggtatagc cctgcgagta ggggatggtc
     8221 tcgccatgca gcttgtcggc cgcgccgccg tagaactcga agtagcgcgc cagggccgtg
     8281 gcgtcggcgc gcgcctgctt ggtgggcttg ccgcagtcgc gcgattcgat ctgcgccagc
     8341 tcttcgtggt ggtcctgcac cttgacggcc agcttgagca gcaggcggct gcgctcggcc
     8401 gggctcaggc gcccccaggc gccgtcgtag gcgcggcgcg cggcctgcac cgccaggtcg
     8461 atgtcgacgg cgtcgccgcg cgcgatgccg tcgtactcct cgccggtgga cgggtcgatg
     8521 acgggaatct gctcgcccga ggcgccggcg cacgcgcggt tgtcgatgaa gtgttgtctc
     8581 atttgatgtc tcctcttcga ttattgtgcc gtgcttaaaa ctgaaccgac aggcctccgt
     8641 cgaccaccag cacgtggccg ttgacgtacg acgccgccgg cgaggccagg aaaacggccg
     8701 cgccggcgat ttcctcgggg agcccccagc ggcccatggg attgcggccc cgcacggcgg
     8761 cgtcgaccgc cgcgtcggcc accatggccg cgttggtttc ggtggcgaag gtgcccgggg
     8821 cgatcgcgtt gctggtaacg cccaggggcg catactccat ggccacggcg cgcatcatgc
     8881 ccgtcaggcc ctgcttggcc gccggataga cggcatcgcc cacgcgcgcg atctcgccga
     8941 ccaccgacgt aatggcgatc agccgcccat agccgtgccg caccatgcgt tcggccgccc
     9001 gcttggacag caggatgccg gccaccaggt ccatctcgat cagctcgcgc acttccgcgg
     9061 gcgtgaacgc ccgcagcgtc ttgcggttgc gggcgccgac attgttgacc aggatgtcga
     9121 gccggccgaa ctcgttgtcg atgcgggcga aggcggcttc ggccgccgca tcgtcggaca
     9181 cgtcgaacgg cagcgccgag gccgccaggc catcggcccg caaagcggcg gcggcctgct
     9241 cgacggcctg cgcgtcgcgg ccgttgagca gcacgtgcgc gccgctgccg gccagggccc
     9301 gcgcgatgca caggcccagg ccgcgcgccg agccggtcac gagggcgacc cggtcaacca
     9361 gggagaattg ccgaagataa ggaagcgtca tgctcgcacc gcagtcggat cagtcccgga
     9421 actccggcgc ccagtgcagc agccgtcgct ccaaccacgt tacagcgtac accaggataa
     9481 tccccaccgc ggacagcagc gccaccgcgg cgaacatgtc ggtggcgttc agggtggcca
     9541 gcgcggtgat catcagatag cccatgcccg tggtcgagcc cacgaactcg gccagcacca
     9601 cgccgatcag cgcgaagctg atggcgttgg gcagcgccgc gaaggtccac gccgccgcgg
     9661 tggggatgcg cacccgccac aggatctgcc gcggcgaggc ccccagggtg cggcagaagt
     9721 ccaccagctc gcgctcgacg ctgcgcccgc ccttgtaggt attgaagaac accaggaaga
     9781 acaccacgaa ccaggacgtc accaccttgg aggccaggcc caggccgaag aacatggtga
     9841 tgagcggcac gaaggcgatg cgcggcatcg agttgaaggc gacgatgaaa ggattgcaga
     9901 cctccgaggc cgtgcggctg cgcgacagcg ccaggcccac ggcgaagccg ctgcccacgc
     9961 cggtcagcag ccccagcagc gtggcctgca gggtcagcag caggtgcggc cacaccgaat
    10021 gcggccccgg ctgcatccat tgccacaggc gcacggccac gcgcgagggc tggctgatga
    10081 agaacgggtc gaagagcgtg ttctgcacga accaggggat gcgcgtcagc ccctcccagg
    10141 cgcccagaat gagggccagg atcagcagtt gccaggcgct gatgcgcagg cggcggcgca
    10201 ccgcggcgcg gcggccctgc agcagctggt ccttgagcgc ggcggcggtc agttcggaaa
    10261 gcgcggcggc ttgctggtcg gacatggcgc tattcccggg catcaggcgg cggcgcggaa
    10321 ctgccgcccg agcaccgacc acaagccctg cagatgcgtc gtgaattgcg gcgtttcgcg
    10381 caaggtgaag acatcgcgcg ggtagggaat atcgatgcgc tcgtcgcgca ggacctggct
    10441 gggcggcccg gaaagcacca ccacgcgggt cgagagcgta acggcttcgt ccaggtcgtg
    10501 cgtcaccatg acgatggtct ggcccagctt gcgccagagg tccatcagct cgcggtgcag
    10561 atgggtcttg gtgtgcgtgt ccagcgcccc gaacggttcg tccagcagca ggatgcgcgg
    10621 atcgaccgcc aggctcatca gcaaggccac gcgccgccgc atgccgccgg agagctggtg
    10681 cggatacgca tcgccgaagc ccgccaggtt gcccagcgcc agcagcgact ccacgcgcgg
    10741 cgcgatgtcc tgttccggcg cgccgtggaa ccgcaggccc agcgcgacgt tcttgcgcac
    10801 cgtgaaccag ggcagcaggg tgtccttctg gaagatatag cccagggccg gcggcacctc
    10861 gtcccgaacg ggccggccct cgacgcgcac gctgccggtg gtcgtgggca ggaagcccgc
    10921 gatcatgttc agcagcgtgc tcttgccgca gcccgacgga ccgacgatgg agacgaactc
    10981 gcccgcctcg atgcgcaggt cgacccggcc caccgcgtgc gtgacgccgt tgcggccctg
    11041 gtaggccttg cccaccccgt cgagctcgat catcgcgtcc gccatcacgc gccccgcgcg
    11101 tggcgcacga agcgcatgtc cacacaggcg gaatacggcg ccttggccag ctcgtcattg
    11161 ccgaactggc ggccgtcgcc catgatggca gtcatgcgcc cgtaggcgtc ctcggtgatg
    11221 atgttgtctt tcaggaagac cgtcttctgg tagacggcta tggttttctc gatcgccggc
    11281 cgcgcgaagc cgctgaggta gtcgtcgtag atggcgtcgg tgacttccgc ggcgccatgc
    11341 gcgctgatga agtcctgcgc cttgaccagg gcgctcacga agccctgcac gacctccggc
    11401 cgcttgtcta tggtctcgcg cagcgtcagc gcggcgatgc cgggcacgtc gccgcccatg
    11461 agctcgttcc aggcggattc ggtcgtggca tcgaagatgg gcacgcccca gccttcctgg
    11521 cgggcctgct ccatcatcga catggtggcc atgctggccg agaccgaacc cgtcttgagc
    11581 gcgcccagca tggtggccag gtcgcccagc ggccggatgt cgaccgtgtc gcccaggccg
    11641 gccttctgca tcagatatac ccccatcagc caggtcaccg actgcggctg ggtcgccccc
    11701 aggcgcttgc cggccaggtc tttcagcgtg cggatcttgc cgctttcgta gtcctcgcgc
    11761 cgcacgatga tgctggcgta ggtcagcttg cggtcgaagc cgaagatcag cgccgcgggc
    11821 ttgccggcca gcgtcagcgc cggcgccgcc gtggcggccg tcgcgccgaa caggatctgc
    11881 ccggcggcca gcaattgatt ggtgcgcggc ccgctctgcg agttcaggta ctcgacctcg
    11941 aggccattgt cggtgaaata gcctttcttc aacgcgaggt agccggcggt gaagaagggg
    12001 tcgatgctgc cctggccgta cacgatcttg accggcgcct gggcgcgcgc gcgcccaggc
    12061 ccgaacagcc cggcggcggc caggccggtg gaggcggcca ggaagcggcg gcgcgacgcc
    12121 gcgccgggca tggattgata gtcggtgttc attgtcgtcc tcacggatat cgttttattt
    12181 atgcggcatg gcctgcggaa cggccatgcc gcgtgagcac gatagcgcaa gtggcgggcg
    12241 gcgcaaacaa aaccgcgccg cctcggcggc cccgatcacc cggcggtttt cttcgccgcc
    12301 tttttcaccg ctttcctggc ggcggtcttc gccgccgtct tcttcacggc agtcttggcg
    12361 gcagccttct tcaccgccac cggcgtttgc gccgaagccg tcttggcggc agtcttggcg
    12421 gccgtcttgc gcgccgggcg ttcgggacgc ggctcgaact cgaagcccac cttgccgtcc
    12481 ggctggcgca ccaggaaagc cttgaacttg cgattcgtgc ggctggagac gaaaccatcg
    12541 agcagatccg tgcgcccatc ggccagcagc ttctccatct gcgcgcgcga gatctcctgc
    12601 tgcaggatca ccttgcccga gcggaaatcg caactctttt ccgggcccac cgacttctcg
    12661 cacacatagc tcatgccgtg ctcgaacacc ttcgagccgc atttcggaca aatgcccacc
    12721 ggcgtatggc ccgagaaatc cacggcctgc gcatcgtcat cgtcgctctg gccgaaatcg
    12781 aactccagct tgtactcgtc gctgatgcgc aaaatggccg cgaaaggccg gcccatcttg
    12841 ctgatgaagc cctgcagcgg gccgatctcg cgcttggcca gcagatcctc cacctcgggc
    12901 agctcgaagg ttcgcccgcc cggatgcttg ctgatcgaga aatcgcaatt cgtgcaggca
    12961 tagcggcggt agttttcctt caccacgccg ccgcacttgg ggcagggcgt ctgcagggtg
    13021 gcgtagtcgc cgggcaccgt gtcgcgttcg tattccttgg cgcgcttgac gatcacctgc
    13081 gtcatctgcg cgatctcgcg catgaaggct tcgcggttca ggctaccctg ctcgatctgc
    13141 ttgagcttgt gctcccactc gccggtgagc tcgggcgagg tgagttccga aacgtccagg
    13201 cccgacagca gcgtcatcag ctggcgcgac ttggccgtgg gcaccaggtc gcggccctcg
    13261 cggcgcagat aggtctcgtt gatcaggcct tcgatgatgg acgcgcgcgt ggctggcgtg
    13321 cccaggccgc gttcggacat ggcctcgcgc agttcctcgt cgtccaccag cttgcccgcg
    13381 ccttccatgg cggagagcaa ggtggcttcg ttgtagcgcg ccggcggctt ggtggtcagg
    13441 cccacggcct cgacttcctc ggtgcgcacc ttctcgccct cggccaccag cgtgagcagg
    13501 gcgtcctcgc cctgggcttc cttgccatag acggccagcc agcccggggc caccaggatc
    13561 ttgccctcgg tcttgaactg atgcccctgc acttcggtga tgcgcgtagt gacgcgatac
    13621 tcggccgccg ggaagaacac cgccagaaag cgcttgagca ccaggtcgta gagcttggcc
    13681 tcggcctcgc tcagctcgcg cggcacctgc agcgtgggaa tgatggcaaa gtggtccgac
    13741 accttcttgt tgtcgaagat gcggcggttg ggcttgaccc actggttctt caccaccgtg
    13801 gccgcatgct gcgacagccc gcccacggcg cgcgaatcgc cctgcgccag cgcctgcatg
    13861 gtctgctgca ccgtggcaac gtaatcctcg ggcaggtagc gcgaatcggt acgcggatag
    13921 gtcagcgcct tgtggcgctc gtacagggtc tgcgccaggg ccagcgtggt cttggccgag
    13981 aagccgaagc ggccgttggc ctcgcgctgc agcgaggtca ggtcgtagag cgccggcgat
    14041 atctgcgtcg acggcttgga ctcttcgcgc acgctgcccg tctggccgcg gcaggcggcc
    14101 accacgctct gcgcggcggc ctgcgaccac aggcgcgact cgcgcctttc cgggtcgcga
    14161 tcgtctttct tgaagtccgg gtcgatccag cggccctcgt acaggccggc ggccgccacg
    14221 aaggtgccgt gcacctccca gtagtcgcgc ggcacgaagc ggcggatgcg ttcctcgcgc
    14281 tcggtcacaa tggccagcgt cggcgtctgc acccggccca ccggcgtctt gaaaaagccg
    14341 ccgtccttgc tgttgaaggc cgtcatggcg cgcgtgccgt tgatgcccac cagccagtcg
    14401 gcctcggcgc gcgaacgggc ggcggcctcc agcggcttca tctgcgtgtc gtcgcgcagg
    14461 ttcaggaagg catcgcggat ggcggcctgc gtcatggact gcagccacag gcgcttgatg
    14521 gccttcttca cccccgcgta ctggatgatg tagcggaaga tcagctcgcc ctcgcgcccc
    14581 gcgtcgcagg cattgatgat ggtgtccaca tccttgcgct tggccagctt gacgagcagc
    14641 ttaagccgct ccgtggagcg cttgtcggcc gggccgaggt cgaactcggg cggaatcacc
    14701 ggcaggtggg tgaagctcca cttgcccttg accggatcgt tcggcgccac caggccgagc
    14761 aggtgcccga cgctggaaga cagcacatat tgatcattct cgaaatagtc gccttcccgc
    14821 gtgaagcccc ccagggcgcg cgagatatcc agagctaccg aaggcttttc ggcgatgatc
    14881 agcgtttttg ttgtcatgag tattccgcat ggcgggagaa ccgccggtta gcgcggataa
    14941 taagtgcggg ccttcagggc atgcaagtcg caagggtacg accctcatta tgtcagaggg
    15001 aaacgtcgtc cccattgggc agtggcctcg gccaggaaaa ccggcacgtc gcgggccggc
    15061 aaggacgcaa aacccagctt gcgccaggcc gcgtccagag ccgccagcgg acggccgacg
    15121 tccagggcct gggcatggtt ctgcttggac agtttaagcc cagtggccgg atcgaccacc
    15181 agcggcacat gcatcacccg cggcagcggc aggtccagca tgcggcccag cacgcgctgg
    15241 cgcgccgtgg agcccagcag atcggcgccg cgcacgacat cggttactgc ctgctcggca
    15301 tcgtcgacca ccaccgccag ctggtaggcc cacagcccgt cggcccgccg cagcgcgaaa
    15361 tcgcccaccg cctgcgccac gtcctgctgc tgcggcccca gccagcggtc gtcgaaccgt
    15421 tcgacccccg gcggcacgcg cacccgccag gcgcgcgcct ggcggccggg agcaaggccg
    15481 tggcggcagg tgccgggata cggccgctcc ccgtcggccc cgagggcgcc gtccggctgc
    15541 gaatcggcga tctcgcgccg cgtgcagccg cagccgtaca ccaggccgcg cgcggccagg
    15601 ccgtcgaagg cccgctggta cagcgcgccg cgcttcgatt gccagacgac atcgccgtca
    15661 cagcgcaggc cgagcgcgcg cagctgggaa aggatgagcg cgtcggcgcc cgccaccgtg
    15721 cgcggcgtgt cgacatcctc gatgcgcacc agccagcggc ccgcccgggc ccgggcgtcc
    15781 agccagctgg ccagcgccgc cgccagcgaa ccggcgtgca agggcccgct ggggctggga
    15841 gcgaagcggc ctatatacat aagaaaccag gaatgaacga ggcgggcgcg acgcccgcct
    15901 tgcagaattt cgggggacgg gaggccctcc ggccggcagc cggtttttgc atcgccccgg
    15961 acagccccgc gccgctcagt gcaggcgccg gccgccttcg tcgtcgagca gctcgtcgag
    16021 cacgaggttg tcgatttcgg cctcctggct ccacaggacc atcagggcga tgatcttggt
    16081 gcacgacagc ggcaccgggg cttccggcgc ggccagggcg cgatcgatga cgatctcgcg
    16141 caagggcgcg ggcaggatgc cggcggattc caggaaggtg atgaaaccga tggactcggt
    16201 acccagctgc cggtactcgg aatcggcgta gatgcgcacg ccctcgctct ggacctgggc
    16261 cagctcgacg cagcgttcgg tggtttcggc caggccgtag agccagccga gcgcatcgtc
    16321 gatgtcctcg tgctcgaaac cggccgcggc gagccgcttg gccagcacgt cagccgtggg
    16381 acaggcttga ggcgtgtagt agttctcgaa caggtaaacc aggatatcga acatatggga
    16441 gttgcctgtt aagagcccat ttgcacagtc ggcccactca tcggcaaacc agcagatgta
    16501 attttactct acgctgattt gtcaccatcg acaacaccgc gcaagccctg aggccgggcc
    16561 tgcgcgtatc ataaaagcga cagcgctcag accgcgccgc gctcgatcac caccccatac
    16621 cagcccaggc cttgataggt ctcgtagccc ggcgtgcggg cgtagccgat caggtcggtg
    16681 tctttcgtgt agaagccgtc catccggtcg gcgggcaggt cgatggtcgc gggaacgtcg
    16741 ctgttattgg ccgcgatcac gtgccggcgc gcgtcgacca ccatgcagcg cacggactcg
    16801 gcctcttcgg ccgacacgcg tatgccgttg accaccgccg aagcctgctt gccccagtcg
    16861 aagaacacgg ccagcacgcc ccgcggcgtg tcgaggcatt cggcatcggc atggatggcc
    16921 gccgcgtaga tggcgctgcg ggcgccgccg agcgccgcca ccggctgcac gtcgcccgcg
    16981 gaataagcgt ccaggccgcg ccccgccagc gcctgcctga accagtcctg gccgctgacg
    17041 tcgtggcccc gcgcggccgg gtaggcatcg ggacggccgt tgcacagcac gcggcccgag
    17101 ggatcgacga cccagatgtc caggtagacg gtataggcgc ccaggatcac gcccaggcgc
    17161 tggcgggtct gggcagccag gtcgtcgtcg tccggctcgg cggccagcgc ctgcacggcc
    17221 gcctcggtgg cccaccagcg cacgtcgcag gtgcgctcgt acaaattgcg gtcgatggtc
    17281 tcgatgacgt tgagcgccat gtcggccagg cgctgcccgc gggaacggta caaggcctgg
    17341 tgcatgctgc cgcccacttc gctcagctcc tgctgcagcg agttggccag cgcctcgacg
    17401 cggtcggaca cttcgcgcac ccggccggcc acgacggcga agctgcggcc cgcctcgccc
    17461 acgtgcgccg cttcgatggc ggcattcagg ctgagcagcc gggtctcgag cgtgatggac
    17521 ttgatttcct ggatcttgac cgatgtgacc cggtcgatgc gcgccgacag ttcggcgacg
    17581 ttttgtgaag gagcttcggt catggcaaaa agctgtctat ccgggatgct gcgtccggta
    17641 ctcgattgaa ggggggcgca ggcggtcacg ccggcaagcg aatcagcgcc gagtgcggca
    17701 ggcggcaccg aacaggctag caccggccac ctggcgccat agcgggaaaa actccctctt
    17761 ctcacccttt cattgctctt tcattgcgtc cccatattag cgtccgccat gggccggcag
    17821 cgccgccctg tcatgccgcg ggcatccacc ggtgctacat ttctttcttt cccgccccgt
    17881 tcgcttcttt acacaggaac cgccatgcaa ttcagccagt tcaacgtcaa cgccctgatg
    17941 gagatcacct cccgtcccga cctggtgttc gtgcgaggcg aaggttcctg gctggaagac
    18001 cacacgggca agcgctacct ggacttcatc cagggctggg ccgtcaacac cctgggccac
    18061 tgcgcgcccg agatgcgcca ggccatcatc gagcagtccg gcaagctcat gaacccctcg
    18121 ccggccttct acaacctgcc ctccatcgag ctggccgagc gcctggccaa gagctcggta
    18181 ttcaagcgcg tgttcttcgc caatagcggc ggcgaggcca acgaaggcgc gatcaagctc
    18241 gcgcgcaagt ggggcaaggt caagaagaac ggcgcctaca agatcatcac catggaccac
    18301 ggctttcacg gccgcaccct ggccaccatg tcggcctcgg gcaagccggg ctgggacacc
    18361 atgttcgccc cgcaggtgcc gggctttccc aaggccgacc tcaacgacct ggattcggtg
    18421 aagaagctca tcgacctgga caccgtggcc atcatgctcg aacccgtgca gggcgaggcc
    18481 ggcgtgtatc ccgccacgca ggcgttcatg cagggcctgc gcaagctggc cgatgaaaac
    18541 cagctgctgc tcatcgtcga cgaagtgcag accggcatgg gccgcaccgg caagctcttc
    18601 gcctaccagc acttcggcat cacccccgac atcatgacgc tggccaaggg catcggcggc
    18661 ggcgtgcccc tggccgcgct gctggcccgc gaagaagtct ccgtgttctc gcacggcgac
    18721 cagggcggca cctacaacgg caacccgctg atgaccgccg tcggcgtggc cgtgttcgac
    18781 aagctcgcct cgcccggctt catggaaagc gtgaataccc gcgccaggca gctctccgaa
    18841 ggcctgctgg ccctctcggc caagtggggc ttcgagggcg agcgtggcct gggcctgctg
    18901 cgcgccctca tcctggacgc cgacatcggc ccggccatcg tcgacgccgc ccgcgaccta
    18961 gccccgcagg gcctgctgct caacgccccg cgcgccaacc tgctgcgctt catgcccgcg
    19021 ctgaacgtga gcgaagccga agtggcgcag atgctggaaa tgctggacgg cgtcatcgcg
    19081 aagctgaaga agtaaaccag cgccctggct cccttcaaca aaacggccac ccaccatttg
    19141 ggtggccgtt ttgcttggca caaatgaggg tgatgttttg atccgttgcg atatccgcaa
    19201 atagccaagc ggcacaccaa attccacact cttggcattg ttgatgatta acatatttgt
    19261 taatattccc agtgccgtac accattagtt attacagcga cgcgttcgct ctggaaatcc
    19321 tggccttgcc gccaaccctg ctggcgcatt atttaaattt aactgaccgc atggttcatg
    19381 caggacccaa tctcggcccc cctcataccg aggcatttgg caatggctta ttccagttgc
    19441 gcctgaaaag agccgaaggc atcagtcgtg ttttctattg caccctggca ggccagcgca
    19501 ttgtgatgct gcatggtttc atcaagaaaa cgaagaagac tcctcctgcc gaacgacgaa
    19561 ttgccgaagc cagattgaaa gaggtcaaac gtgatcacac atgaaaaact cagggcccga
    19621 gctctcgcca atcccgccgt acgcgccgaa tacgagcgca tcgaacggga agaaatgccc
    19681 atgctcgacg ccatcctcaa ggcccgcgcg gaagcagggc tgacgcaggc acaagtggcc
    19741 gagcgcatgg gcaccaaggc gcctgccgtg gcgcgcctgg aaaacgcatt aattaccgga
    19801 gcgccatccc catcgctcaa taccctgcgc aaatatgccg ccgcgctggg caaacggctg
    19861 gaaatacgcc tggtctagct gtgaagagtc aagaggttat atccgtcgag gttgagtctg
    19921 gagatgggga cagcgcggcc gatgccctgg tgtggtcgat gccagttgta gtggtgtagc
    19981 caggatttca tggcatcggc tcgatgttgg gagtt
//
LOCUS       DASWPO010000221        19376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2550873, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000221 DASWPO010000000
VERSION     DASWPO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 19376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19376
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550873"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VGC69_11815"
     CDS             <1..670
                     /locus_tag="VGC69_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0296008.1"
                     /translation="EAMLVIDKPAGVAVHGGSGVSFGVIEQLRAARPEAKFLELVHRL
                     DRETSGLLMVAKKRNALLALHAMLREGQGGKHYYALVEGDWVNDRQHIKLPLTKWTTA
                     SGERRVKVDREGGLAAHTIVTLQQRYGGYSLVDAELRSGRTHQIRVHLASNGFPIVGD
                     DKYGRDETRAAMAKQGFNRMFLHAHNLTLPHPLTGETLALNAPLPEACVRLLKQLDRQ
                     TETD"
     gene            670..1320
                     /locus_tag="VGC69_11820"
     CDS             670..1320
                     /locus_tag="VGC69_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0296009.1"
                     /translation="MPYSLVVFDWDGTLMDSTHDIVAAIQAACRDLELPVPSASEASW
                     VIGLSLESALQRAVPKLTPSLLPRFLERYRIHYLLRDPDLRLFDGVNEMLDALSARNV
                     KLAVATGKSRVGLNRVLSATGLGTRFDTTRTADETFSKPNPAMLHQIMSELDVAPERV
                     VMVGDTSHDLQMASNAGIHGLGVTYGAHAPGELTACAPQAVLDNVPELQAWLLDRV"
     gene            1432..2379
                     /locus_tag="VGC69_11825"
     CDS             1432..2379
                     /locus_tag="VGC69_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0296010.1"
                     /translation="MALRKIGQSSLQVAPLMLGGNVFGWTADEATSFSVLDAFVDAGL
                     NFIDTADVYSAWAPGHQGGESETLIGKWLARSGKRDRVVIATKVAKYARRPGLSAANI
                     QAAVEDSLKRLQTDCIDIYFSHEDDPKVPIEESLGAYQKLIQAGKVRVIGASNFSGER
                     TREALRVAQANGLPRYAILQPEYNLYSRAAYEAELEPVALAEQLAVVPYFSLASGFLS
                     GKYRSEADLSKSVRGGRAVKNYLNPRGFGILAALDQVAERHGATQASVALAWLMARPS
                     ITAPIASATSVEQVSSLAAAVRLKLGADDIRALDAASAT"
     gene            2401..3381
                     /gene="msrP"
                     /locus_tag="VGC69_11830"
     CDS             2401..3381
                     /gene="msrP"
                     /locus_tag="VGC69_11830"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEY0296011.1"
                     /translation="MLIQRSSDILPSEITDEAVWQARRQWLKQSIRGAAALGLGTGLG
                     YSAGAQAAAAPDDAPLPGTPSPQYALMDKPTSFDDVTHYNNFYEFGLDKSDPAETAGK
                     LITRPWTVSVEGEVAKPRTFDIDELLKLAPMEERVYRLRCVEGWSMVIPWEGYSLSTL
                     LKQVEPTGNAKYVEFVSVVQKETMPGVSSRVLDWPYVEGLRLDEAMNPLAMLVFGLYG
                     KVLPKQNGAPLRLAVPWKYGFKSAKSLVTIRLVEKQPETAWVNAASNEYGFYANVNPD
                     VPHPRWSQATERRIGDSGGPFAPKRKTLMFNGYADQVASLYAGMDLKANF"
     gene            3383..3991
                     /gene="msrQ"
                     /locus_tag="VGC69_11835"
     CDS             3383..3991
                     /gene="msrQ"
                     /locus_tag="VGC69_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEY0296012.1"
                     /translation="MAAPALTVRTVGRIKPLLFIVGLLPFLRWLWLGWHDGLTANPVE
                     FLTRSSGTWTLVCLLVTLGITPLRRLIGQPALVRLRRMCGLFAFFYALMHFLTWAWWD
                     QDWVPSSMIEDVVRRPFITVGFTAFVLMSLLAVTSPQAAMRKLGRNWQRLHYAIYVIG
                     LLAILHFWWMRSGKNNFEAPLIYGGVLLVLLGWRVLARLRRG"
     gene            complement(4050..4796)
                     /locus_tag="VGC69_11840"
     CDS             complement(4050..4796)
                     /locus_tag="VGC69_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296013.1"
                     /translation="MQPRLKAPDTTHTPMYLRIRNAIRDAITQGAYKPGDLLPSETEL
                     AKRYGTTRATVVHAMQQLVFEGLIDRRRGLGSFVAPSALGTVVDTRQIGYFERDIHAS
                     GQNLTYEVVGFGKAPVDDQIRNALRLDAKEPVFRLQRLRIVHAAPIAFEMRYMPALIG
                     TQLTHDQLVHHPVQDLLEQHVGLHIQQYENTIRVALPPASVAKHLKIDRGRPVLVRAY
                     TWLDARGIPLLWGETLYREEYQIHYMLESK"
     gene            4986..6215
                     /locus_tag="VGC69_11845"
     CDS             4986..6215
                     /locus_tag="VGC69_11845"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HEY0296014.1"
                     /translation="MNTSHDDTFSIARFRVDLGLGGYWINDQAAVQTSAKPDGFFFEG
                     ATSSPVFDAVRAPSLAYLISLELDDGRVAFGDCVTVANAGYAGRPAPLRRASAAALEA
                     ALNEQLTRRRYGGFREAAQVLQEISLPQAIELPARYGVSQALLDAAAQAKRTTMMRVL
                     CDEYGLPVPTEPFGFAGSCGGAWEANVDKAIVRKVAMFPQSAIQTVAECERLPGYVTW
                     IRERIDKWGGEGYLPDLHFDFHSSLGRMLDNDETRIIDYLAGICEKAGPCRVYFEDPV
                     LSKGPAEAIERMGSLRAKLQARLENARLVADEWANGPGQVAAFAAAQASHAIQIKMPD
                     NGSLLESIAAMQACKQHGVLAYLGGSCNETDISARASVHVGLAFGAWRMFTKPGLGFD
                     EGLMIMANEVSRTLGRG"
     gene            6252..7241
                     /locus_tag="VGC69_11850"
     CDS             6252..7241
                     /locus_tag="VGC69_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296015.1"
                     /translation="MMTSRLLRPAAMALLFGLGLAMADSSAMAQGFPNHIVTLIVPFP
                     PGAATDMVARTVAQQLSIKWNQSVLVENRAGATGGIGSNYVARSAPDGYTLLVATSSS
                     HTMGPNMIKKQLWDPIKDFSPVTLLTWAPNVLTVNPSVPAKTIPELIALLKKNPGKYT
                     FASSGTGSSIQLAGELFKKLAGVDMVHVPYKGAAPALADLLGGQVNMMFDTVAESLPL
                     IKSGKLRALAVTTPRRSSVLADIPTMQEAGVPGYEMSAWIGLMAPANTPPDVVDKIGR
                     DTRAVLAMPEVESRFAEAGMEAAPMSSDEFAARIKMELPTYGKLMRDAGIQPE"
     gene            7291..8670
                     /locus_tag="VGC69_11855"
     CDS             7291..8670
                     /locus_tag="VGC69_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296016.1"
                     /translation="MDDVLSPDVAQATRWLGGLATRVRYADLPAPARRIVLHCIVDWY
                     AVTLAAAGHPALAPLLDDAGREGGRPLATAVGMAEKLGLYPAALINGTTAHFLDYDDV
                     NLAITGHPTAVVYSALLPLAQYRRSSGPEVLDAFVAGYETACRLGRWLGDSHYEHGYH
                     ATATAGVVAAAAACARLMRLDADRMACALGLAATQACGLKAQFGTLGKPLHAGLAARN
                     GLMAACLAAGGYDGSLGVLEAVQGYAATLSPAPDFAAAAAEPAGGWHLYGRLFKYHAA
                     CYGTHAGIECARALRAGGLDPATVSAVRVVAHVSGRNMCNIAAPLSANEARFSLRLNI
                     AFALLGIDTGDIHAYADERLRHPDVISLRDRIEVDFSDDMQMMEASIEVALLDGRTLQ
                     VRKDAGLPEQDVPVEENRIRAKFLSLAAPVLGAGAAQGLLERLFAFAGLPDLDCVADV
                     LAAPGGARA"
     gene            8667..9887
                     /locus_tag="VGC69_11860"
     CDS             8667..9887
                     /locus_tag="VGC69_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296017.1"
                     /translation="MTLGRGRARHILPVIVISQFGGTSLWFAGNAVAGDLAAGLSPAY
                     AASAVQLGFIAGTLFSARLRLADRYAAPALFFVCCLLGALFNYPVTALARGAGGYAVP
                     AVLAARFVVGLTLAGIYPVGIKVAAGWYREGLGGALGYLTGALVLGTAFPHMVRGLGA
                     AVPWNAVLAAASMAAVAGGALLWALVAEGPYVAPRLARDAIRPQVVPGFSPWSAVRRQ
                     ARLRASACGYFGHMWELYAFWAFLPVVLAAHGTVDAWTTELVSRWSFAVIAVGAIGCI
                     VGGLVANRRGSAPVALACLVVSGVCCLASPWAYGLPMPAFLAFLAVWGIAVVGDSPQF
                     SALNALESPRNQVGSVVTLVNSIGFAITVVSIQLLAWLLPAIGPRWLLLPLVAGPVVG
                     CLVFAQGYFRASRD"
     gene            complement(9923..11452)
                     /locus_tag="VGC69_11865"
     CDS             complement(9923..11452)
                     /locus_tag="VGC69_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0296018.1"
                     /translation="MNVFRFSSSHGLAVGLALALSSCASVGPDFHAPEATAPADWRAR
                     PTSAAELAASVAPSTDPLPASWWEVFGDPTLDALQARAIQASPDLQTATLRFAQARLQ
                     RRVAASQALPQVDLDAQAARQRQSEESAETRLAGALGGASAPQLVQVLSEPFSLYQAG
                     FDVSWELDLWGRVRRLVEAADAGIEAAQAQLQDTRLVLSSELARAYFELRQVQRQQAL
                     LTRDVAVARDLLALRQAQAANGLTDADPPLAQSQRVAQLAAQRAALQARQAALDNQIG
                     LLTGDAPGALNALLAPASADLDQDAPPGLPALALGQPADLLRRRPDVRGAEARLHAAT
                     AEIGVAVADLYPRIGFDASAGFQSLDAADLGDWGSRTWQVGPVLSLPVFDRGRRRANV
                     ELRRKDQQLAAVAWHQRVLKSWQEVDDALNDYAAEHARNARLREREAASREQLTFARV
                     RAGTGLGNDLSVLQAELAWRQTQADVAESDCALQTALVRVYKASGGGTAPAPGVANAS
                     P"
     gene            complement(11462..12571)
                     /locus_tag="VGC69_11870"
     CDS             complement(11462..12571)
                     /locus_tag="VGC69_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296019.1"
                     /translation="MIDYLRQLANLCRKEFLAVLKDPANRVILFVPVILQSLLFGYGA
                     TFDLTDVPYAVVDQSHGEHSMRLLAKLDGTGKFHLVATLHSPAQIADVIDAGDALLVL
                     HFAADFDRALDNQGTAPLQVILDGRNSTTAGNAAGQVGAIVAAFNATLPNAARPPITV
                     EAHAWFNPNLETRWNIVPGLIAALSMIQVMMLAALSVAREREQGTFDQLLVTPASPGQ
                     IMIGKAVPAIVIGLVQSALVVLIARYWFRVPMAGDVLSLAAGLLVFTIAVVGIGLSIS
                     ALSANMQQAMLYTFVLLVPLVLLSGLATPVGNMPEAFQVATYANPLRFAIDLVRRVYL
                     EGEPLGQVWRDFIPFVCLALLTLPLASWLFRNRLV"
     gene            complement(12580..13737)
                     /locus_tag="VGC69_11875"
     CDS             complement(12580..13737)
                     /locus_tag="VGC69_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296020.1"
                     /translation="MTDAQPRGGYDGTRGVRRRLVALIRKEFHQLLRDRSNLLIGIGL
                     PLVLILIFGYGLSLDVRDARVVVVLEDPSPAAHDIVAALDGSRYFSTTVVRSLAEGRH
                     LMADHEAEALLHLQSDFARHANDADARVQLLLQGGDPLRSAAIRSYVESAVALMAQKD
                     QDRQGGAALARGVAVVDRMWFNAANNSTWYLVPGLIVLIMTLVGTFLTALVMAREWER
                     GTLESLFVTPVRPIEVLIAKIVPYFAVGMAGLGMCLVAAKLLFGVPIHGSLAVLLGAG
                     MLYMLVSLALGLLISALTRNQFLASQVAILASFMPSMMLSGFVFDLRNVPQPVRLVGN
                     LLPATYFMELVKTLFLAGTYWPIVLKDCTILALYAVALLTLARVVTRKRLD"
     gene            complement(13734..15533)
                     /locus_tag="VGC69_11880"
     CDS             complement(13734..15533)
                     /locus_tag="VGC69_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0296021.1"
                     /translation="MSESDSPVIDARGVGKHFSDPATRRPVIALEDLSIQAARGAITA
                     LVGPDGAGKTTFLRLVAGLMRPDQGRLAVLGLDVAQHPQAVQDRISYMPQRFGLYEDL
                     SVQENLDLYADLHAVPLSARRERFARLLEMTDLAHFTRRLAGKLSGGMKQKLGLACTL
                     VRSPELLLLDEPTVGVDPLSRRELWEIVQRLVVQEHLSVLLATTYMDEAERCARVHVL
                     HEGRLLTSGDPQEIAGHAHGLTYRADPPAGQPPRLLQARLLDDDAAIVDAVPNGGRVH
                     FIRRPSADPAALGLPAGQWRPVDAKLEDGFMVLLRARQSERPSTAQIATPDAVDAPQP
                     ARDGATVIEVRELVRRFGDFIAVDKTTFSVKRGEIFGLLGPNGAGKTTTFRMLCGLLP
                     ASGGEARVAGMDLRTAGARARSRIGYVSQKFALYANLTVRENLQFFGGAYGLRGAALR
                     ERMHTVLGQFDLQGQELQPAGQLPGGVKQRLAMAVGLLHEPEILFLDEPTSGADPLAR
                     RSFWRRITAFASAGTTIVITTHFMEEAEYCDRIVIQDAGKLLALGTPEQVRRQAGETA
                     DSRLDMEQAFIRIVETARAEREANAPRAAEAAA"
     gene            complement(15530..16549)
                     /locus_tag="VGC69_11885"
     CDS             complement(15530..16549)
                     /locus_tag="VGC69_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY0296022.1"
                     /translation="MTRKRVLAGALLAVILIAGALYAWHRQRGTGSDVLTLYGNVDIR
                     QVALAFDGNDRIAEMRAEEGDRVQPGQVLAVLDTRTDTLQLDQANAQVGVLEQTLLAL
                     HHGSRPEEIAQAAAQVAAAEAEVQRSRLQLQRLQQTSAKTEGRAVSRQDLDNAQAQLR
                     VALAQRDVQRQAQNLARIGPREEEIARATAQLQAARAEQALWRHRIDLALLRAPQAAI
                     VRSRLLEPGDMASPQHPAYTLALTDPKWVRAYVNEAELGRIHQGMAASVITDGAPGSP
                     ISGRVGFISSVAEFTPKNVQTEELRTSLVYEVRILVDDPRDALRLGMPATVRIDAGSR
                     AQTPP"
     gene            complement(16662..17438)
                     /locus_tag="VGC69_11890"
     CDS             complement(16662..17438)
                     /locus_tag="VGC69_11890"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0296023.1"
                     /translation="MMRRLAPALTLALAGLLAGCATGPVPIDDSIRAVGQDSRVRYVV
                     LHYTDLPADASLRVLSQQQVSVHYLITDANPPHVYRLVPETRNAWHAGASSWYGEIEL
                     NNTSIGIEIVNLANSAGAWQPYTQGQIDTLLPLLRDIVQRHGIAPQNIVGHSDIAPQR
                     KIDPGPLFPWRLLAQNGLGRWYDEAAQAAQLARLQNEPLPDVSWFQQQLQRLGYECPQ
                     DGQLTPATRRVIAAFQMHYRPSRYDGTPDAETAAIMIALK"
     gene            complement(17474..18196)
                     /locus_tag="VGC69_11895"
     CDS             complement(17474..18196)
                     /locus_tag="VGC69_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0296024.1"
                     /translation="MNAPGRGALHLIPVGLGEAPVARWLPGDVRALAARLDTYIAENA
                     KTARAFLKQMDTERPLQEIVIHTLSPDTDAAQIAQWLKPLHAGAEIGLVSEAGCPAVA
                     DPGARVVAEAHRQGLAVRPWVGPSSILLGLMASGLDGQRFAFHGYAPVDPAERAKQLR
                     AWEQHSARHDQTQMLIETPYRNAAMFKTMLGALRGDTRLCIARSLTTEDEQVRTRTVA
                     DWKQQPAPDLDKKPTLFLFLAR"
     gene            complement(18264..18875)
                     /locus_tag="VGC69_11900"
     CDS             complement(18264..18875)
                     /locus_tag="VGC69_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HEY0296025.1"
                     /translation="MPAAPVPPRLILASSSRYRREMLSRLRLPFSAVSPNIDETPHPG
                     EAPADLALRLSVEKAQAVAADHPGAVIIGSDQVATVDGQPIGKPGSFERAVEQLTRLS
                     GREVQFHSALAVTDGTRVERADIVTYCRFRPLSQAAIEAYLHAEKPFDTAGSAKAESL
                     GIALMASMRSDDPTAIIGLPLIALTGMLASFGLDPLQYAGAQA"
     gene            18917..>19376
                     /locus_tag="VGC69_11905"
     CDS             18917..>19376
                     /locus_tag="VGC69_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296026.1"
                     /translation="MFLQALFGLAQGESIVKDADPRNIDAFALARKGGTLSGETAIVR
                     FLRLVEDLPEQNGLARWQVRGSMGMGTAKTGVIAGQPLLHLHAQADLLLDCQRCGQPF
                     TFAVDAQVVLQLVESEADLDDELGLLQEDGADEIDANPFFDESGGDEDVAA"
BASE COUNT         3043 a         6372 c         6600 g         3361 t
ORIGIN      
        1 cgaagccatg ctggtcatcg acaagccggc cggcgtggcg gtgcatgggg gcagcggcgt
       61 gtcgttcggc gtcatcgaac aactgcgcgc ggcccggccc gaggcgaaat tcctcgaact
      121 ggtgcaccgg ctcgaccgcg agacgtcggg cctgctcatg gtggccaaga agcgcaacgc
      181 cctgctggcc ctgcacgcca tgctgcgcga aggccagggc ggcaagcatt attacgcgct
      241 ggtcgaaggc gactgggtca acgaccggca gcacatcaag ctgccgctga ccaaatggac
      301 gaccgccagc ggcgagcgcc gcgtcaaggt cgaccgcgaa ggcggcctgg cggcgcatac
      361 aattgtcacg ctgcagcaac gctacggcgg ctacagtctc gtcgacgccg aactgcggtc
      421 cgggcgcacc caccagatcc gggtgcatct ggcgtccaac ggctttccca tcgtcggcga
      481 cgacaaatac ggccgcgacg aaacccgggc ggcaatggcc aagcagggtt tcaaccgcat
      541 gttcctgcat gcgcacaacc tgaccctgcc gcatccgctc acgggcgaga ccctggcgct
      601 gaacgcgccg ctgcccgagg cctgcgtgcg gctcctgaag caactcgata ggcaaacgga
      661 gacggattga tgccttattc gctggtggtg ttcgactggg acggcacctt gatggattcc
      721 acccatgaca tcgttgccgc catccaggcg gcctgccgcg acctcgaact gcccgtgcct
      781 tcggcctcgg aagccagctg ggtgatcggc ctgtcgctcg aaagcgcgct gcaacgcgcc
      841 gtgcctaagc tgacaccgtc gctgctgccg cgctttctgg agcgctatcg catccactat
      901 ctgctgcgcg atcccgatct gcggcttttc gacggcgtca acgagatgct cgacgccctg
      961 tcggcgcgca acgtcaagct ggccgtggcc accggcaaga gccgggtggg cctgaaccgg
     1021 gtgctgagcg ccacgggcct gggcacgcgt ttcgatacca cccgcacggc cgacgaaacc
     1081 ttcagcaaac cgaatcccgc catgctgcac cagatcatga gcgagctcga cgtggcgccc
     1141 gagcgcgtcg tgatggtggg cgacacctcg cacgacctgc agatggccag caatgccggc
     1201 atccacggcc tgggcgtcac ctacggcgcg catgcgcccg gcgaactgac ggcctgcgcg
     1261 ccgcaggcgg tgctggacaa cgtgccggaa ctgcaggcct ggctgctcga cagagtttga
     1321 ttacggccgg tttcccggcg cacgacatgc cgggcgggga aagggtatcc tgatcgtttc
     1381 ggcccctccg cctctcggcg catgggtctt gtcacatcag ggggaaacct catggcatta
     1441 cgcaaaatcg gtcagtcgtc cctgcaagtc gcgccgctga tgctgggcgg caatgtcttc
     1501 ggctggacgg ccgatgaagc cacctcgttt tcggtgctgg acgcttttgt cgacgccggc
     1561 ctgaatttca tcgacacggc cgacgtctat tcggcctggg cgcccggcca ccagggcggc
     1621 gagtcggaaa ccctcatcgg caagtggctg gccaggagcg gcaagcgcga ccgcgtggtg
     1681 atcgccacca aggtggccaa gtacgccagg cggcccggcc tgtcggcggc caatatccag
     1741 gccgccgtcg aagactcgct caagcgcctg cagacggact gcatcgacat ctacttttcc
     1801 cacgaagacg atcccaaggt gcccatcgaa gaatccctgg gcgcctatca aaagctgatc
     1861 caggcgggca aggtgcgcgt catcggcgca tccaatttca gcggcgagcg cacgcgcgag
     1921 gccttgcgcg tggcccaggc caacggcctg ccgcgctacg caatcctgca gcccgaatac
     1981 aacctctaca gccgcgcggc ctacgaggcc gagctcgagc cggttgccct ggccgagcag
     2041 ctcgccgtgg tgccgtactt cagcctggct tccgggttcc tgtccggcaa ataccgttcg
     2101 gaagccgact tgtcgaaaag cgtgcgaggc ggccgggccg tcaagaacta cctcaatccg
     2161 cgcggattcg gcatcctggc ggcgctggac caggtggccg agcgccacgg cgccacgcag
     2221 gcctcggtgg cgctggcctg gctgatggcg cggccgagca tcacggcgcc catcgccagc
     2281 gccacgtccg tcgaacaggt gtccagcctc gcggcggccg tgcggctcaa gctcggcgcc
     2341 gacgacatcc gggccctgga cgccgccagc gcaacctgaa cgcgcacaag aggtgtggtc
     2401 atgctcatac agcggtcttc cgacatcctg ccctccgaaa tcactgacga agccgtgtgg
     2461 caggcgcggc ggcagtggct caagcaatcg atccggggcg ccgccgcgct ggggctgggc
     2521 acgggtctgg ggtactcggc cggcgcgcag gccgctgccg cgccggacga cgcgccgctg
     2581 cccggcacgc ctagtccgca gtacgcgctg atggacaagc cgacctcgtt cgacgacgtc
     2641 acccactaca acaacttcta cgagttcggc ctggacaagt ccgatccggc cgaaaccgcc
     2701 ggcaagctca tcacccggcc ctggacggtg agcgtcgagg gcgaggtcgc caagccccgc
     2761 accttcgaca tcgacgaact gctcaagctc gcgcccatgg aagagcgcgt ctaccggctg
     2821 cgctgcgtcg aaggctggtc gatggtgatt ccctgggaag gctattcgct gtccacgctg
     2881 ctcaagcagg tcgagcccac gggcaatgcc aagtacgtgg agttcgtcag cgtggtgcag
     2941 aaggaaacca tgcccggcgt gagttcgcgg gtgctcgact ggccctacgt cgaggggctg
     3001 cgcctggacg aggccatgaa tccgctggcc atgctggtgt tcggcctgta tggcaaggtg
     3061 ctgcccaagc agaacggcgc gccgctgcgc ctggccgtgc cctggaaata cgggttcaag
     3121 tcggccaagt cgctggtgac gatacgcctg gtcgagaagc agcccgagac ggcctgggtc
     3181 aatgcggcgt cgaacgaata cggtttctat gccaacgtga atcccgacgt gccgcatccg
     3241 cgctggagcc aggccaccga gcgccgcatc ggcgattcgg gcggcccttt cgcgcccaag
     3301 cgcaagacgc tgatgttcaa cggctatgcc gaccaggtcg cttcgctcta cgcgggcatg
     3361 gacctgaagg ccaatttctg acgtggcggc ccccgcgctc actgtgcgga cggtcggccg
     3421 catcaagccc ttgctgttca tcgtcggcct gctgcctttt ctgcgctggc tgtggctggg
     3481 ctggcacgat gggctgacgg ccaacccggt cgagttcctg acgcgttcgt cgggtacctg
     3541 gacgctggtg tgcctgctcg tcacgctggg catcacgccg ctgcgccgct tgatcggcca
     3601 gccggcgctg gtgcgcctgc gccggatgtg cgggctcttc gcctttttct atgccctgat
     3661 gcattttctc acctgggcct ggtgggacca ggactgggtg ccgtcctcga tgatcgagga
     3721 tgtggtgcgg cggcccttca tcacggtggg ttttaccgcc ttcgtattga tgagcctgct
     3781 ggcggtcacg tcgccgcagg cagccatgcg caagctgggc cgcaattggc agcgcctgca
     3841 ttacgcgatc tatgtcattg ggctgttggc catcctgcac ttttggtgga tgcggtcggg
     3901 caagaataat ttcgaagcgc cgttgatcta tggcggagtg ttgcttgtgc tgctggggtg
     3961 gcgggtgctt gcccggctgc ggcgagggta atcgccgcga attcctggct ccatggacgc
     4021 ctcgatagtc cgagagcttt tcaacaaatc tatttcgatt ccagcatgta gtggatctgg
     4081 tattcctcgc ggtagagcgt ctcgccccac agcaggggta ttccgcgcgc atccagccac
     4141 gtataagcgc gaaccagcac cggacggccc cggtcgatct tcaggtgttt ggctaccgag
     4201 gctgggggga gcgcgacgcg tatggtgttt tcgtattgct ggatgtgcag gccgacatgc
     4261 tgttccagca agtcctgcac cggatgatgg accaattggt cgtgcgtcaa ttgcgtgccg
     4321 atcaaggcgg gcatgtagcg catctcgaaa gcgatgggcg cggcatgcac gatgcgcagg
     4381 cgctgcagcc ggaacacggg ctccttggcg tccaggcgca gggcgttgcg gatctggtcg
     4441 tcgaccggcg ctttgccgaa gccgaccact tcgtaggtca ggttttggcc gctggcgtgg
     4501 atgtcccgct caaaatagcc tatctgccgg gtgtccacga cggtgcccag ggcagacggc
     4561 gcgacaaagc tgcccaggcc gcgccgccgg tctatgaggc cttcgaagac gagttgctgc
     4621 atcgcgtgca cgacggtggc gcgggtggtg ccgtaacgct tggcgagctc tgtttcagag
     4681 ggcagcagat cgcccggttt gtaggcgcct tgcgtgatcg catcccggat ggcgttgcgg
     4741 atgcgcaaat acatgggggt atgcgtggtg tcgggcgctt tgagcctggg ctgcatggag
     4801 agggcctgtg ggcgttggtc cgtgtccggg tgggcacgga tttgaatgta ttgattgtat
     4861 agtcaactac aagtatagtt gccccattca aggctcggcg cgaggtgctc gactgtgagc
     4921 agcgaagaca tctgccgggc ttttttgtag ctcaaattga ttagtcaact agttgtattg
     4981 gcaatatgaa cacatcacac gacgacacgt tttccatcgc ccggttccgg gtggatttag
     5041 gcctgggcgg ctattggatc aacgaccagg cggccgtcca gacatcggcc aagccggacg
     5101 gttttttctt tgaaggcgcc accagttcgc ccgttttcga cgcggtccgc gcgcccagcc
     5161 tggcctatct gatatcgctg gagctggacg acgggagggt cgctttcggc gattgcgtca
     5221 ctgtcgccaa tgccggctat gccgggcgcc cggcgccgct gcgtcgagcg tctgcggccg
     5281 cgctggaggc cgcgttgaac gagcaactga cgcggcggcg ctacggcggc ttccgcgagg
     5341 ccgcacaggt cctgcaggaa atatcgctgc cgcaggcgat cgaactgcct gccaggtatg
     5401 gcgtttccca ggctttgctg gacgccgccg cgcaggccaa gcggaccacg atgatgcgcg
     5461 tgctgtgcga tgaatacggc ctgccggtgc ctaccgagcc gttcggtttc gccggttcct
     5521 gcggcggagc ctgggaagcc aatgtggaca aggccatcgt tcgcaaagtg gcgatgttcc
     5581 cgcaatcggc catccagacc gtcgccgagt gcgagcggct gcccggctat gtgacctgga
     5641 tacgggaacg gatcgacaag tgggggggcg aaggctatct gcccgacctg catttcgatt
     5701 tccattcttc gctgggtcgc atgctcgaca acgacgaaac ccggatcatc gactatctgg
     5761 ccggtatctg cgagaaggcg ggaccgtgcc gggtgtattt cgaagatccg gtcctgtcga
     5821 agggtccggc cgaggcgatc gagcgcatgg gttcgttgcg agccaagctg caagcacggc
     5881 tggagaacgc caggctggtc gctgacgagt gggccaatgg tcctggccag gtggctgctt
     5941 tcgccgctgc gcaagcctcg cacgccattc agatcaagat gcccgacaac gggtcgctgc
     6001 tcgagtccat cgcggccatg caggcttgca agcagcacgg cgtgcttgcc tacctggggg
     6061 gcagttgcaa cgaaaccgat atttcggctc gggcttctgt ccatgtgggg ctggcgttcg
     6121 gcgcctggcg catgttcacc aagccgggcc tgggctttga cgaaggcctg atgatcatgg
     6181 ccaacgaggt cagccggacg ctggggcgcg gctgattatt ttcctccaca acaatttcca
     6241 aggagacaac aatgatgaca tcgcgactgc tccgtccggc ggctatggcc ctgctgttcg
     6301 gcttgggtct cgccatggcg gactcgtccg ccatggcgca gggctttccc aaccatatcg
     6361 tcacgctgat cgtgcctttt ccgccgggcg cggcgaccga tatggtcgcg cgcacggtgg
     6421 cgcagcagct gtcgatcaag tggaaccagt cggttctggt cgagaaccgc gcgggggcca
     6481 cgggaggcat cggttccaac tatgtcgcgc ggtcggcgcc cgacggctat acgctgctgg
     6541 tcgccacgag cagcagccat acgatggggc ccaacatgat caagaagcag ctatgggacc
     6601 cgatcaagga tttctcgccc gtcacgctgc tgacctgggc gcccaacgtg ctgacggtca
     6661 acccttccgt gccggccaag accatacccg aattgatcgc cttattgaaa aagaaccccg
     6721 gcaaatatac cttcgcctcg tccggcacgg gcagttccat ccagctcgcc ggagagcttt
     6781 tcaagaagct ggccggcgtc gacatggtgc acgttccgta caagggggcg gcgccggccc
     6841 tggccgattt gctgggcgga caagtcaaca tgatgttcga taccgtggcc gaatcgctgc
     6901 cgctcatcaa gtccggcaag ctgcgcgcgc tggcggtgac gaccccgcgc cgctcgtcgg
     6961 tgctggcgga cattcccacc atgcaggaag ccggcgtgcc aggctatgaa atgtcggcgt
     7021 ggatcggcct gatggcgcca gccaacacgc cgcccgacgt ggtcgataag atcggccgcg
     7081 atacgcgggc tgtcctggcg atgcccgagg tcgagagccg cttcgccgaa gccggcatgg
     7141 aggcggcgcc catgtccagc gacgagttcg ctgcgcgcat caagatggag ctgccgacct
     7201 acggcaagct catgcgggat gcgggcattc agcccgaatg acggcacatc gccctgtgcg
     7261 ctgggcctgt cgtgcgatgg aggtttgatt gtggatgacg ttctttcacc cgatgtcgcg
     7321 caagcgactc gctggttggg cgggcttgcc acgcgcgtcc ggtacgccga tctgccggcg
     7381 ccggcgcggc gcattgtcct gcattgcatt gtcgactggt acgccgtgac gctggcggcg
     7441 gccggccatc cggccctggc gccgctgctc gatgacgccg ggcgcgaggg cggaaggccg
     7501 ttggccacgg ccgtcggcat ggcggaaaag ctgggcttgt atccggccgc gctcatcaac
     7561 ggcacgacgg cgcacttcct ggactacgac gacgtcaatc tggccatcac cgggcatccc
     7621 actgcggtgg tgtattccgc cctgttgcct ttggcgcaat accggcgcag cagtggaccg
     7681 gaggtgctcg atgccttcgt ggccggctac gaaaccgcct gccgtctcgg ccgctggctg
     7741 ggcgactccc attacgagca cggctaccat gcgaccgcga ccgctggcgt ggtggccgcc
     7801 gccgcggcat gcgcgcggct gatgcggctg gatgcggacc ggatggcgtg cgcgctgggg
     7861 ctggctgcga cccaggcctg cggcctgaaa gcgcaattcg gcacgctggg caagcccctg
     7921 cacgccggct tggccgcccg caatggatta atggcggcct gcctggccgc cggcggttat
     7981 gacggcagcc ttggcgtgtt ggaggcggtc cagggttatg ccgcgacgct gagcccggcg
     8041 ccggacttcg ccgccgcggc cgccgagccg gccggtggat ggcatctgta cggccgtttg
     8101 ttcaagtatc acgcggcctg ttacggcacg cacgcgggca tcgaatgtgc ccgtgccctg
     8161 cgggcaggcg gacttgaccc ggcgacggtg tcggccgtgc gcgtcgtggc gcatgtcagc
     8221 ggccgcaata tgtgcaatat cgcggcgccg ctctcggcga acgaagcgcg cttcagcttg
     8281 cgcctgaata tcgccttcgc cctgctgggc atcgacactg gcgatatcca tgcctatgcg
     8341 gatgaacggt tgcggcatcc ggatgtgatc agcctgcgcg accgtatcga agtggatttt
     8401 tccgacgata tgcaaatgat ggaagcttcg atcgaggtcg cgctgttgga cggccggacg
     8461 ctgcaggtgc gcaaggacgc gggtctgccc gagcaggacg tgccggtcga ggaaaaccgg
     8521 atacgggcca aattcctcag cctggcagcg cctgttctgg gggccggcgc cgcgcaaggc
     8581 ttgctcgagc gcctgtttgc ctttgccggc ctgccggacc tggattgcgt ggcggatgtg
     8641 ctggccgcgc ccggcggcgc ccgcgcatga cgctaggtcg cggcagggcc cgccacatac
     8701 tgcctgtcat cgtcatttcg cagttcggcg gaacctcgct ctggttcgcc ggcaacgcgg
     8761 tggccggcga tttggccgcg gggttgagcc cggcctacgc ggccagcgcg gtgcaactgg
     8821 gcttcatcgc cggcacactg ttttccgctc gcctgcgcct ggcggaccgc tacgccgcgc
     8881 cggcactttt ctttgtctgt tgcctgctcg gcgcgctgtt caattatccc gtgaccgcgt
     8941 tggcgcgtgg cgccggcggc tatgccgttc ctgccgttct cgcggcccgc ttcgttgtcg
     9001 gtctgacatt ggccggcatc tatcccgtgg gcatcaaggt cgccgcgggc tggtatcgcg
     9061 aggggctggg cggagcgctg ggctatctga cgggcgcgct ggtgctcggt acggctttcc
     9121 cgcacatggt ccgggggctg ggagcggcgg tgccatggaa tgccgtgctg gctgctgcgt
     9181 ccatggccgc cgtggcgggc ggcgcgttgt tgtgggcctt ggtggctgaa ggtccatacg
     9241 tcgcgccccg gctggcacgc gatgcgatcc ggccgcaggt tgttcccggc ttttcgccgt
     9301 ggagcgccgt gcggcggcaa gcccggctgc gagcttcggc ctgcggctat tttggccata
     9361 tgtgggagct gtatgccttc tgggccttcc tgcctgtcgt gcttgccgcg cacggcaccg
     9421 tcgatgcctg gacgacggag ctggtgtcgc gatggtcgtt tgccgtgatc gcggtgggtg
     9481 cgatcggatg catcgtgggc ggcctggttg cgaatcgccg cggcagcgcg ccggtggcgc
     9541 tggcttgtct ggtcgtttcc ggggtgtgct gtctggcttc gccttgggcg tatggcctgc
     9601 ccatgccggc attccttgct tttctggccg tttggggaat cgctgtcgta ggggactcac
     9661 cgcagttctc cgccttgaat gcgctggagt ccccgcgcaa tcaggtcggc agcgtcgtga
     9721 cgctggtcaa cagcataggc ttcgccatca cggtcgtcag tatccaattg ctggcttggc
     9781 tgctgcccgc gatcggcccg cgctggctgc tgctcccttt ggtcgctggc cctgtggtgg
     9841 gttgcctggt gttcgcgcaa gggtatttcc gggcgagccg agactagcgt gatgaaggcc
     9901 ggctgaggcg ttcgacatcg tgtcacgggg aggcgttcgc aacgcctgga gccggcgccg
     9961 tcccgccgcc cgacgccttg tagacccgca ccagcgcggt ctgcaaggcg cagtcgcttt
    10021 ccgccacatc ggcctgtgtc tggcgccagg ccagttccgc ctgcagcacg gacaggtcat
    10081 tgcccagtcc cgtcccggcg cgcacgcggg caaaggtcag ttgctcgcgg ctggctgctt
    10141 cgcgctcgcg caggcgcgcg ttgcgcgcgt gttcggcggc gtaatcgttg agcgcgtcat
    10201 ccacttcctg ccaggacttc agcacacgct ggtgccaggc gactgccgcc agttgctgat
    10261 ccttgcggcg caattcgaca ttggcccggc ggcgtccccg gtcgaatacc ggcaggctca
    10321 gcacgggccc cacctgccag gtgcgcgacc cccagtcgcc caagtctgcc gcatcgaggg
    10381 actggaatcc agccgaggca tcgaacccga tgcgcggata caggtcggcc accgcgacgc
    10441 cgatttccgc cgtggccgca tgcaggcgcg cttcggcgcc gcggacatcg ggacggcggc
    10501 gcagcagatc cgccggctgc cccaatgcca gggccggcag gcccgggggc gcgtcttgat
    10561 cgagatcggc gcttgccggc gcgagcagcg cattcaatgc gcccggcgca tcgcctgtga
    10621 gcaacccgat ctgattgtcg agcgcggcct ggcgagcctg caaagcggcc cgttgcgcgg
    10681 ccagttgcgc gacccgctgc gattgcgcca gcggcgggtc cgcgtcggtc agtccgttgg
    10741 cggcctgggc ctgccgtagt gccagcaggt cccgggcgac cgctacatcg cgcgtcagca
    10801 gcgcctgctg gcgctggacc tggcgcagtt caaaatacgc gcgggccagc tcgctggaca
    10861 gaaccaggcg cgtgtcctgc aactgcgcct gcgcggcctc gatgccggcg tctgcggcct
    10921 cgaccaggcg cctgacacgg ccccacagat ccagttccca cgaaacgtcg aacccggcct
    10981 ggtacagcga gaaaggctcg ctgagcacct gcaccagttg cggcgcgctg gcgccgccca
    11041 gcgcgccggc gagtcgtgtt tccgccgatt cctcgctttg cctttggcgc gcagcctgcg
    11101 cgtccagatc cacttgcggc aaggcctgcg aagccgccac gcgccgctgc aggcgggcct
    11161 gagcgaagcg cagggttgcc gtctgcaagt cggggctggc ctggatcgcg cgcgcctgca
    11221 gcgcatccag cgtcggatcg ccaaatactt cccaccagct cgcgggcagc ggatcggtgg
    11281 acggcgccac ggacgccgcg agttccgccg cgctggtcgg gcgggcgcgc cagtccgccg
    11341 gcgccgtcgc ctcgggcgca tgaaaatcgg gccccacgct cgcgcacgag gacagcgcga
    11401 gcgcgagtcc caccgccagc ccatgcgaag aagagaatcg gaacacgttc atgatgacca
    11461 ctcagacgag acgattgcgg aacagccagc tcgccagcgg cagcgtcagc agcgccaggc
    11521 agacgaacgg aatgaagtcg cgccacacct gcccaagcgg ctcgccctcc agatacacgc
    11581 ggcggaccag gtcgatcgcg aagcgcaaag gattggcata ggtcgcgacc tggaatgcct
    11641 cgggcatatt gcctaccggc gtggccagcc ccgagagcag cacgagcggc accagcagca
    11701 cgaaggtgta tagcatcgct tgctgcatgt tcgccgacag cgccgagatg gacagtccga
    11761 tgccgaccac cgcgatcgta aaaaccagca ggccggccgc gagcgacagc acgtccccgg
    11821 ccatcggcac gcggaaccag tagcgcgcga tcagcacgac cagcgcggac tggaccaggc
    11881 cgatgacgat cgccggcacc gccttgccga tcatgatctg gccaggcgag gccggcgtca
    11941 ccagcaattg gtcgaaagtg ccttgctccc gttcgcgcgc caccgacagc gccgcgagca
    12001 tcatgacctg gatcatgctc agcgccgcga tcaggccggg cacgatgttc cagcgcgtct
    12061 ccagattcgg gttgaaccac gcatgcgcct ccaccgtaat cggcgggcgg gccgcgttcg
    12121 gcagcgtggc gttgaaagcc gcgacgatcg cgcccacctg gcccgcggca ttgccggcgg
    12181 tggtcgaatt gcgcccgtcc aggatcacct gcaatggcgc cgtgccctgg ttgtccagcg
    12241 cgcggtcgaa gtcggccgcg aagtgcagca ccagcagcgc gtcgccggca tcgatcacat
    12301 cggcgatctg cgccggggag tgcagggtgg cgacaagatg aaacttgccc gtgccgtcga
    12361 gctttgcgag caggcgcatc gaatgttcgc cgtggctctg gtccaccacg gcatacggca
    12421 cgtcggtcag gtcgaaggtc gcgccgtagc cgaacagcag gctttgcagg atgaccggca
    12481 cgaacaggat cacgcggttg gccggatcct tgagcaccgc gaggaattcc ttgcggcata
    12541 gattggcgag ctggcgcagg tagtcgatca tcgcgtgcct caatcgagac gcttgcgcgt
    12601 cacgacccgc gcgagcgtca gcagcgccac cgcatacaag gcgaggatcg tgcagtcctt
    12661 gagcacgatc ggccaatacg tgccggcgag aaatagcgtc ttgaccagct ccatgaagta
    12721 ggtcgccggc agcaggttgc cgaccagccg cacaggctgc ggcacgttgc gcaggtcgaa
    12781 tacgaagccc gacagcatca tcgaaggcat gaagcttgcg agaatcgcga cctggctcgc
    12841 aaggaactgg ttgcgcgtga gcgccgagat cagcagcccc agcgccagcg atacgagcat
    12901 gtacagcatc ccggcgccga gcagcaccgc cagcgagccg tggatcggca cgccgaacag
    12961 cagcttcgcg gccacgaggc acatccccag tccggccatg ccgaccgcga aatacggcac
    13021 gattttcgcg atcagcacct cgatgggcct caccggcgtg acgaacagcg actccagcgt
    13081 gccgcgctcc cattcgcgcg ccatcaccag ggcggtgaga aacgtgccga ccagcgtcat
    13141 gatcagcacg atcagcccgg gcaccagata ccaggtgctg ttgttggcgg cgttgaacca
    13201 catgcggtcg accacggcga cgccgcgcgc cagcgccgcg ccgccctggc ggtcctggtc
    13261 tttttgcgcc atcagcgcga cggcgctttc gacatagctg cggattgccg ccgagcggag
    13321 cggatcgccg ccttgcagca gcagctgcac ccgcgcatcc gcgtcattgg cgtgccgcgc
    13381 gaaatcgctt tgcaggtgca gcagcgcttc ggcttcgtga tcggccatca ggtgccgccc
    13441 ttcggccagc gagcgcacga ccgtcgtcga gaaatacctg gacccgtcca gcgcggcgac
    13501 gatgtcgtgt gcggccggag agggatcttc gagcacgacc acgacacgcg cgtcgcgcac
    13561 atccagcgac aacccatagc cgaagatcag gatgagcacc agcggcaagc cgatgccgat
    13621 cagcaggttg ctgcgatccc gcagcagctg gtgaaactcc ttgcggatca gcgcgacgag
    13681 tcgccgccgg acgccgcgcg tcccgtcgta gccgccgcgt ggctgtgcgt ccgtcatgcc
    13741 gccgcctccg ccgcgcgcgg cgcgttcgct tccctttcgg cgcgcgccgt ctcgacgatg
    13801 cggatgaacg cctgttccat atccagccgg ctgtcggcgg tttcgccggc ttgccggcgg
    13861 acctgttccg gcgtccccag cgccagcagc ttgcccgcat cctggatgac gatgcggtcg
    13921 caatattccg cctcctccat gaaatgcgtg gtgatcacga tcgtggttcc ggcgctcgcg
    13981 aacgcggtga tgcgccgcca gaagctgcgt cgcgccagcg gatcggcgcc gctggtcggt
    14041 tcgtcgagaa acaggatctc cggctcgtgc agcagcccga ccgccatcgc cagccgctgc
    14101 ttgacgccgc cgggcagttg cccggccggc tgcaattcct gtccttgcag atcgaactgc
    14161 ccgagcacgg tatgcatccg ttcgcgcagc gccgcgccgc gcaggccata cgcgccgccg
    14221 aagaactgca ggttctcccg caccgtcagg ttcgcgtaca gcgcgaattt ctgcgacacg
    14281 tagccgatcc ggctgcgcgc ccgcgcgcct gccgtgcgca gatccatgcc ggccacgcgc
    14341 gcctcgccgc cgctggccgg cagcaggccg cagagcatcc ggaacgtggt cgtcttgccc
    14401 gcgccgttgg ggccgagcag tccgaagatt tccccgcgct tcacgctgaa ggtcgtcttg
    14461 tcgactgcga tgaaatcgcc gaaacgacgc accagttcgc gcacctcgat caccgtggcg
    14521 ccatcgcggg cgggctgcgg cgcatcgacc gcgtccggtg tcgcgatctg cgccgtcgac
    14581 gggcgctcgc tttggcgcgc gcggagcaag accatgaagc cgtcttccag cttggcgtcc
    14641 actggccgcc attgccccgc gggcaggccc agcgcagccg gatcggcgct gggccgccgg
    14701 atgaagtgca cgcgtccgcc gttgggcacc gcgtcgacga tcgccgcatc gtcatcgagc
    14761 aggcgcgcct gcagcaggcg cggcggctgt cccgccggcg gatccgcgcg gtaggtcagg
    14821 ccatgcgcgt gcccggcaat ctcttgcgga tcgccgcttg tcagcaaacg gccttcgtgc
    14881 agtacgtgaa cccgcgcgca acgctcggcc tcgtccatgt aggtcgtggc cagcagcacc
    14941 gacaggtgtt cctggacgac cagccgctgc acgatctccc ataattcgcg ccgcgacagc
    15001 ggatcgacgc cgacggtcgg ttcgtcgagc agcagcagtt ccggcgaacg caccagcgtg
    15061 cacgcaaggc cgagcttctg cttcatgccg ccggacagct tgcccgcgag gcgccgggtg
    15121 aagtgcgcca ggtcggtcat ctccagcagg cgggcgaagc gttcgcggcg cgccgacagc
    15181 ggcaccgcat gcaggtcggc gtatagatcg aggttttcct gcacgctcag gtcctcgtac
    15241 aggccgaagc gttgcggcat gtagctgatg cggtcctgga ccgcctgggg atgttgcgcc
    15301 acgtccagcc cgagcacggc gagccgaccc tggtccgggc gcatcagacc cgccaccaga
    15361 cgcaggaaag tcgtcttgcc ggcgccgtcc ggcccgacca gcgccgtgat cgcgccgcgc
    15421 gccgcctgga tggacaggtc ttcgagcgcg atgaccgggc ggcgcgtcgc cggatcgctg
    15481 aaatgcttgc cgaccccgcg tgcgtcgatg actggcgagt cgctctcgct catggaggcg
    15541 tctgcgcgcg cgagccagcg tcgatccgca ccgtggccgg catgccgagg cgcaaggcgt
    15601 cgcgcggatc gtcgacgagg atgcgcacct cgtacacgag gctggtgcgc aattcctcgg
    15661 tctgcacgtt cttgggcgtg aattcggcta cggatgagat gaaaccgacg cgtccggaaa
    15721 tcggcgatcc cggcgcgccg tcggtgatca cgctggccgc catgccctga tggatgcggc
    15781 ccagctcggc ctcgttcaca taggcgcgca cccatttcgg atcggtcagc gccagcgtgt
    15841 aggccgggtg ctgcggcgac gccatgtcgc ccggctccag caggcgggaa cgcacgattg
    15901 cggcctgcgg cgcgcgcagc agggcgaggt cgatccggtg ccgccacaac gcctgttcgg
    15961 cacgcgccgc ctgcagttgc gccgtggcgc gcgcgatctc ttcctcgcgc ggcccgatgc
    16021 gcgcgaggtt ctgcgcctgc cgctgcacat cccgttgcgc cagcgccacc cgcaattgcg
    16081 cctgggcgtt gtccagatcc tggcggctga ccgcgcgtcc ttcggttttc gcgctggttt
    16141 gctgcaagcg ctgcagttgc aggcggctac gctgaacctc cgcctcggcc gccgccacct
    16201 gcgcagccgc ctgcgcgatt tcctcggggc ggctgccgtg atgcaaggcc agcaaggtct
    16261 gttccagcac gccgacctgg gcgttcgcct ggtccagctg caaggtgtcg gtgcgcgtat
    16321 cgagcacggc caggacttgc ccgggctgga cgcggtcgcc ttcctcggcg cgcatctcgg
    16381 cgatccggtc gttgccatcg aaagccagcg caacctggcg gatgtcgaca ttgccgtata
    16441 gcgtcagcac atcgcttccg gtcccgcgct gccgatgcca tgcgtacaac gcgccggcaa
    16501 tgaggatgac ggccagcaat gcacccgcaa gaacacgctt tctggtcatg gaaggatgcc
    16561 tggctccggg aggatgccgc cattctaatg ggttatttcc gcgccgattc cgtgtttggc
    16621 atgcggctcc ggctgcgcgc ggcgggccct gggactatcc ctcacttcaa cgcgatcatg
    16681 atcgccgccg tttccgcatc cggcgtcccg tcatagcgcg acggacggta atgcatctgg
    16741 aaggccgcga tcacgcgccg cgtggcgggc gtgagctgtc catcctgcgg gcactcgtac
    16801 cccaggcgct gcagttgctg ctggaaccag gagacgtcgg gcagcggctc gttctgcagg
    16861 cgcgccagct gcgcggcctg cgccgcttcg tcataccagc gtcccagccc gttctgcgcc
    16921 agcaagcgcc acgggaagag cggcccggga tcgatcttgc gctgcggggc gatgtcgctg
    16981 tgccccacga tgttctgcgg cgcgatgccg tggcgttgca cgatgtcgcg cagcagggga
    17041 agcagcgtat cgatctgccc ctgcgtgtag ggctgccagg caccggcgct gttggccagg
    17101 ttgacgatct cgatgccgat cgacgtgttg ttcagctcga tctcgccata ccaggaactg
    17161 gcgccggcat gccaggcgtt gcgcgtctcg ggcaccaggc ggtagacatg cggcggattg
    17221 gcatcggtga tcaggtagtg gacgctgacc tgctgctggg acagcacgcg cagcgaggcg
    17281 tcggccggga ggtcggtgta atgcagcacc acgtagcgca cgcggctgtc ctggccgacg
    17341 gcccgtatgg aatcgtcgat gggcacgggg cccgtggcgc agccggcgag caggccggcc
    17401 agcgccaggg tcaacgcggg cgccaggcgc cgcatcatgc gcgacaggcc gttgggcagg
    17461 cagcggaaca cgatcatcgc gccaggaaca ggaacagcgt gggtttcttg tccaggtcgg
    17521 gcgcgggctg ctgtttccag tcggccacgg tgcgcgtccg cacctgctcg tcttcggtgg
    17581 tgagcgagcg ggcgatgcac agccgggtgt cgccgcgcag cgcgcccagc atggtcttga
    17641 acatggccgc gttgcgataa ggcgtctcga tcagcatctg cgtctggtcg tgcctggccg
    17701 aatgctgctc ccaggcgcgc agctgtttgg cgcgttcggc cgggtctacc ggggcgtagc
    17761 cgtggaaggc gaagcgctgg ccgtccaggc cgctggccat caggcccagc aggatggagg
    17821 aggggcccac ccagggccgc acggccaggc cttgccggtg cgcctcggcc accacgcggg
    17881 cgcccgggtc ggccacggcc gggcagccgg cctcggacac caggccgatc tcggcccctg
    17941 cgtgcagggg cttgagccat tgcgcgatct gggcggcatc cgtatcgggg gacagggtgt
    18001 ggatgacgat ttcctgcaac ggccgctcgg tgtccatctg cttgagaaag gcgcgcgccg
    18061 tcttggcgtt ttcggcgata taggtgtcga gccgcgcggc cagggcgcgc acgtccccgg
    18121 gcagccagcg cgccacgggg gcctcgccca gccccacggg gatgaggtgc agggcgccgc
    18181 ggcccggcgc gttcatgctg cccccttggc gctgcgcgcc atccccccaa gggggaatga
    18241 acgctcgagg cggctcggcg cgttcatgcc tgggcccctg cgtattgcag cggatcgagg
    18301 ccgaaggacg ccagcatgcc ggtcagggcg atcagcggca ggccgatgat ggcggtgggg
    18361 tcgtcgctgc gcatgcttgc catcagggcg atgcccaggc tctccgcctt ggcgctgccg
    18421 gccgtgtcga acggcttttc ggcgtgcagg taggcctcga tggccgcttg cgacaggggg
    18481 cgaaagcggc agtacgtgac aatatcggcg cgctccacgc gcgtcccgtc ggtgacggcc
    18541 agggcgctgt ggaattgcac ctcgcggccc gacaggcggg tgagctgctc gacggcgcgt
    18601 tcgaagctgc cgggcttgcc gatgggctgg ccgtcgacgg tggccacttg gtccgaccct
    18661 attatgacgg cgcccgggtg atccgccgcc acggcttggg ccttttccac cgacaggcgc
    18721 agggccaggt cggccggcgc ctcgccggga tggggggtct cgtcgatatt gggggacacg
    18781 gcggaaaagg gcaggcgcag tcgggacagc atctcgcgcc gatagcgcga actcgaggcc
    18841 aggatcaagc ggggaggaac aggggctgca ggcattctat cttttgacaa aaaactgtaa
    18901 gtcacggtat tatctaatgt ttttgcaggc cttgtttggc ttggcgcagg gcgaatcaat
    18961 tgtgaaagac gccgaccccc gcaatatcga tgcttttgct ttggcccgca agggcggcac
    19021 cttgtctggc gagacggcca tcgtgcgctt tttgcgcctg gtcgaggacc tgcccgaaca
    19081 gaacgggctt gcccgctggc aggtgcgggg ctcgatgggc atgggcacag ccaagaccgg
    19141 cgtgattgcc ggccagccgt tattgcacct gcatgcgcag gctgacctgc tcctggattg
    19201 ccagcgttgc ggccagcctt tcaccttcgc ggtggatgcg caggtcgtgc tgcagctggt
    19261 cgagtccgaa gcggatctcg acgacgagct gggtctcctg caagaagacg gagcggatga
    19321 gattgacgcg aacccgttct ttgacgagag cgggggcgat gaagatgtcg ccgcgg
//
LOCUS       DASWPO010000222        10578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2641536, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000222 DASWPO010000000
VERSION     DASWPO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641536"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VGC69_11910"
     CDS             complement(<1..478)
                     /locus_tag="VGC69_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0296027.1"
                     /translation="MNQSILEVADLSARYGKVGALAGASLSVPAGRIVTVIGANGAGK
                     STLLNAVMGSLPQTGHASGAVRYDGGEVQGWQAERRVAVGMSLVPEKRELFGTMSVED
                     NLLLGGFRRYRARERNWRATLDEVYDLFPRLRERRVQQAGTLSGGERQMLAVGRALM"
     gene            complement(546..2384)
                     /locus_tag="VGC69_11915"
     CDS             complement(546..2384)
                     /locus_tag="VGC69_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY0296028.1"
                     /translation="MSAAKNRIALWVFLAALVVLPLASATPEFWITQLNYIGLAALVA
                     MGLMLLTGVAGLTSFGQAAFVGLGSYATAYLTTRYGASPWLGLVAGLALTGLVAWLLG
                     LITLRLSGHYLPLGTIAWGLSLYYLFGNLEALGEHNGIAGIEPISFFGVSLLEGRRIY
                     FLIWVCVLLAAWASSNLLDSRPGRAIRALKSGANMAESMGVNTASYKVVVFVWAALLA
                     SLSGWLYAHMQRTVSPSPFGLEVGIEYLFMVVVGGSGSVWGAILGAALILVIKDQLQN
                     LLPLLLHTDANFELIVFGVLMIVVLQYASNGLWPLVAGGWSRVLGTAGSRRGRAAPAE
                     APALARRARPAAGQAVLEVSGLRKQFGGLVAVNDISFTVRAGEIMGLIGPNGAGKSTT
                     FNLVSGVAPATRGDVRFLGEAISGLSARSVAMRGVGRTFQHVQLLPTMTVIENVALGA
                     HLRSQVGVFAGALHGDRAREAQLLHEAARQLQRVGLGDYLYEQAGNLALGQQRILEIA
                     RALASDPVLLLLDEPAAGLRYKEKQELAQVLEQLRDEGLSILLVEHDMDFVMRLANHL
                     VVMDFGTKLAEGLPAEVRENPAVLEAYLGGIDDDLPEEVRSAESQA"
     gene            complement(2381..3418)
                     /locus_tag="VGC69_11920"
     CDS             complement(2381..3418)
                     /locus_tag="VGC69_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296029.1"
                     /translation="MDFSIALILLQDGIVNGAIYALLGMALVLVFAVTRVIFIPQGEF
                     VAFGALTLAMLVDGRVPGTVYLLPMLGLVVLLLELLRALRSGIWSGLPAVLGSCVALP
                     LLLLWLTRAYAGPNTSLWVDMLLSLLLVVPMGPMVYRIVYTPLAEASVLVLLIVSVAV
                     HFTLTGLGLIFFGAEGWRTPTFMSGQVNLGFMSWSAQSLFVVGVCLLLILALWLFFGK
                     TLYGRALRATAVNRRGARLVGISTAMSGGLTFTLASAIGAVSGMLIAPLTTIYYDTGF
                     LIGLKGFVAAIIGGLASYPVAAAGALLVGVLESFSSFWASSYKEVIVFTLIIPVLVWR
                     SFNNRPTDDEE"
     gene            complement(3486..4646)
                     /locus_tag="VGC69_11925"
     CDS             complement(3486..4646)
                     /locus_tag="VGC69_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296030.1"
                     /translation="MMKHLSAAAAALVFLGLPLAANAQIKVGVTLSSTGPAASLGIPE
                     RNAAALLPKEADGQKIEWIVLDDATDPTAAVRNARKLATEDKVDVLIGTSAVPGSLAM
                     VDVAAELKVPMISVAASAAIVSPMDDKRHWAFKTPQNDALMAGALADAMAKAKVKTLG
                     FIGFSDAYGDSWLNAMQQAGEAKGIKIVAVEKYGRTDTGVTGQALKLVAAHPDAILIA
                     GAGTPAALPQKELKARGYAGTIYQTHGAANNDVLRVCGKDCEGMLLPAGPLLVAAQLP
                     DSNPVKKSAMAFVQAYEQAYGAGSTNPFGGHVWDAGQLILAALPVALQTGAKPGTPAF
                     RAAMRDAIEGVKNLPASQGVFNMSPTDHAGFDERSRVMVKVEGGKWVYQPDL"
     gene            complement(4684..5844)
                     /locus_tag="VGC69_11930"
     CDS             complement(4684..5844)
                     /locus_tag="VGC69_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296031.1"
                     /translation="MRKAGLQKIAAALVLALPLAAVAQVKVGVTLSSTGPAASLGIPE
                     RNAANLLPKEVAGQKIEWIVLDDATDTTAAVRNARKLATDDKVDVLVGTSVTPGSLAM
                     VDVAAELKIPVISVAASAQIVEPVDDKHRWAFKTPQNDALMAGALADAMDKAKVKTLG
                     FIGFSDAYGDGWLSVMQQAAKAKGINIVEVEKYSRTDTSVTGQVLKVVAAHPDAVLIA
                     GAGTPVALPQKELKARGYTGLIYQTHGAANIDVLRVCGKDCEGMLLPAGPLLVAAQLP
                     DSNPVKKSAMAFVQAYEQANGAGSINTFGGHMWDAGQLIINALPVALKTGAKPGTPAF
                     RAAMRDAIEGIKNLAASQGVFNMSPTDHAGLDARAHVMVKVEGGKWVYQPDL"
     gene            6179..6577
                     /gene="msrB"
                     /locus_tag="VGC69_11935"
     CDS             6179..6577
                     /gene="msrB"
                     /locus_tag="VGC69_11935"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0296032.1"
                     /translation="MEKVRKTDAEWRALLTPQEFSVAREKATERAFTGRYWDTFTPGI
                     YRCVACGTALFASDTKFDAGCGWPSYFEPLGPGLVREEKDASHGMVRTEVLCNICDSH
                     LGHVFPDGPPPTGLRYCINSLSLSFEPQSK"
     gene            6574..7170
                     /locus_tag="VGC69_11940"
     CDS             6574..7170
                     /locus_tag="VGC69_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY0296033.1"
                     /translation="MKKFLFDLFPLILFFVAYRYADIYAATAVAVVASIAQIVWLKAT
                     RKPIEVTHWINLGVIVLFGGATLWLHSDVFIKWKPTALYWLFGAVLIGGRFTGRNPMR
                     KLLGQQIAMSEAAWNRLNLSWALFFAAAGLINLYVAFSGHFTEAQWVNFKVFGLMGLL
                     LVFVIGQSIWLSRHMQPDPQLDEAGPADEAGKNTGNTP"
     gene            7167..7460
                     /locus_tag="VGC69_11945"
     CDS             7167..7460
                     /locus_tag="VGC69_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY0296034.1"
                     /translation="MTVDRIALIRERLAALSPASLDIQDDSHLHAGHAGAQGGAGHYQ
                     VRIVASCFKGLSAVARHRLVYDRLNDLMPHTIHALAIDARPPVETTPETSKAH"
     gene            7507..8283
                     /locus_tag="VGC69_11950"
     CDS             7507..8283
                     /locus_tag="VGC69_11950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0296035.1"
                     /translation="MKRIIMLAAACAIAVPAFAQNVATVNGKPISQKSMDDFVKLLVN
                     QGATDSPQLRDQVKEEMINRQVFVQAAEKQGLAKQASVQTEVELARQGILVRALMTDY
                     LAKHPVTDADVQTQYDKVKQQESGKMEYKVRHILVKDEKTADDLLAQIKADKSKFEDL
                     AKKNSQDPGSSGNGGDLGWAPATNYVPPFADAVTKLKKGEITDKPVQTQYGWHIIMVD
                     DTRPVEFPPLAQVKPQIEEMLRQQTLGDYQKSLRDAAKIQ"
     gene            complement(8594..>10578)
                     /gene="ligA"
                     /locus_tag="VGC69_11955"
     CDS             complement(8594..>10578)
                     /gene="ligA"
                     /locus_tag="VGC69_11955"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0296036.1"
                     /translation="EHNVRYYVHDEPSISDADYDALMRELQALEAAHPELLTPDSPTQ
                     RVGAAPLAEFGSVRHAVPMLSLGNAFESDDVEAFDKRVADTLHGAGLLAAEAQVDYFC
                     ELKLDGLAISLRYENGELVQAATRGDGQEGEDVTSNIRTIRAIPLRLLGAPPRVLEVR
                     GEVLMNRADFERLNQAQAARGEKVFVNPRNAAAGSLRQLDPRITAQRPLRFFAYGWGE
                     IPEDAGLPRDTHGGMLGWLAGFGLPVNAKHNHRAQGAAGLLAFYEHVGRIRPGLPYDI
                     DGVVYKVDALAAQQVLGFVARAPRFAVAHKFPAEEATTELLGIEVQVGRTGAITPVAR
                     LKPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDVIPEVVGPVLEKRVGDPP
                     PFVMPTACPVCGSAIEKLEDEAIARCTGGLFCAAQRRQTLLHAAGRKALDIEGLGEKL
                     VDQLVDGGRVKSLADLYGLKAEELAALDRMGEKSAENLVQAIAQARQPALGRLLFALG
                     IRHVGETTARDVARHFGTIEAIMDADEEALLAVRDVGPVVAGSIRRFFAEPHNREIIA
                     QLRGQGVEPVAEAEPQGASLAGKTFVLTGAMPTWSRDEATRHILAAGGKVSGSVSKKT
                     AYVVAGEEAGSKLAKAQELGVAVLSEDELKALLGLL"
BASE COUNT         1792 a         3711 c         3415 g         1660 t
ORIGIN      
        1 ccatcaatgc gcggcccacc gccagcatct ggcgttcgcc gcccgagagc gtgccggcct
       61 gctggacgcg gcgctcgcgc aagcgcggaa acaggtcgta gacctcgtcc agcgtcgcgc
      121 gccaattccg ttcacgcgcc cgatagcggc gaaaaccgcc caggagcaga ttgtcttcca
      181 ccgacatggt gccgaacagc tcgcgctttt ccggcaccag cgacatgccg accgccacgc
      241 ggcgctcggc ctgccagccc tgcacctcgc cgccgtcgta acgcaccgcg ccgctggcgt
      301 ggccggtttg cggcaaggag cccatcaccg cgttgagcag cgtggacttg cccgcgccgt
      361 tggcgccgat cacggtgacg atgcggcccg ccggcaccga cagcgacgcg ccggccaagg
      421 cgccgacctt gccgtagcgc gccgacaggt cggccacttc gagtatggac tggttcatgt
      481 tgccctcttg gcgctacgcg ccgtcccccc gagggggaat gatcgcttga ggcggctcgg
      541 cgcgttcatg cctggctctc cgcgctccgg acctcttccg gcagatcgtc gtcgatgccg
      601 cccaggtagg cctccagcac cgccggattc tcgcggacct cggcgggcag tccctcggcc
      661 agcttggtgc cgaagtccat caccaccaga tggttggcca ggcgcatgac gaaatccata
      721 tcgtgttcca ccagcaagat gctcaggccc tcgtcgcgca gctgttcgag cacctgggcc
      781 agctcctgct tttccttgta gcgcaggccg gcggcgggct cgtcgagcag cagcagcacc
      841 ggatcgctgg ccagcgcccg cgcgatctcc aggatgcgct gctggcccag ggccaggttg
      901 ccggcctgtt cgtagagata gtcgcccagg cccacgcgct gcagttggcg ggccgcctcg
      961 tgcagcaact gcgcctcgcg ggcacggtcg ccgtgcaggg cgcccgcgaa cacgccgacc
     1021 tggctgcgca ggtgcgcgcc cagggccacg ttctcgatca cggtcatggt gggcaacaac
     1081 tgcacgtgct ggaaggtacg gcccacgccg cgcatggcga cgctgcgcgc cgacaggccg
     1141 ctgatggcct cgcccaggaa acgcacgtcg ccccgggtgg ccggcgccac gccgctcacc
     1201 aggttgaagg tggtgctctt gcccgcgccg ttggggccga tcaggcccat gatctcgccg
     1261 gcgcgcaccg tgaaggaaat atcgttgacc gccaccaggc cgccgaactg cttgcgcagg
     1321 ccgctcacct cgagcacggc ctgccccgcc gccggccggg cgcgccgtgc cagggccggc
     1381 gcctcggccg gcgcggcccg cccacggcgc gaacccgccg tgccgagcac gcgcgaccac
     1441 ccgccggcca ccaggggcca caggccgttg ctggcgtact gcagcaccac gatcatcagc
     1501 acgccgaaga cgatgagctc gaaattcgcg tcggtgtgca gcaagagcgg caacaggttt
     1561 tgcagctggt ccttgatgac gaggatcagc gcggcgccca ggatggcccc ccacacgctg
     1621 cccgaaccgc ccaccaccac catgaacagg tactcgatgc cgacttccag gccgaaggga
     1681 ctggggctga ccgtgcgctg catgtgcgca tacagccatc ccgacaggct ggccaggagc
     1741 gcggcccaga caaagaccac gaccttgtac gaagccgtgt tcacgcccat ggactcggcc
     1801 atgttggcgc cgctcttcaa ggcgcggatg gcgcgcccgg gacgcgagtc gagcagattg
     1861 ctgctggccc aggccgccag cagcacgcag acccagatca ggaagtagat gcgccggcct
     1921 tccagcaggg agacgccgaa gaatgaaatg ggctcgatgc cggcaatgcc gttgtgctcg
     1981 cccagcgctt cgagattgcc gaacaggtaa tacagcgaca ggccccaggc aatggtgccc
     2041 agcggcaggt agtggcccga caggcgcagg gtgatgagcc ccagcagcca ggccaccagg
     2101 ccggtcagcg ccaggcccgc caccaggccc agccacggcg aggcgccgta ccgggtggtg
     2161 agataggccg tggcatagga tcccagcccg acgaaggccg cctgcccgaa ggaagtcagc
     2221 ccggccacgc cggtcagcag catcaggccc atggccacca gggcggccag gccgatgtag
     2281 ttgagctggg tgatccagaa ctcgggcgtc gcggacgcca gcggcagcac caccagcgcg
     2341 gcgaggaaca cccacagggc gatacggttc ttggcggcgc tcattcttcg tcgtccgtgg
     2401 ggcggttgtt gaacgaacgc cagaccagca ccgggatgat cagcgtgaac acgatgactt
     2461 ctttatagga gctggcccag aatgacgaga acgactccag cacgcccacc agcagcgcgc
     2521 cggcggcggc caccgggtag ctcgccagcc cgccgatgat ggcggccacg aagcccttca
     2581 ggccgatcag gaaacccgtg tcgtagtaga tcgtggtcag cggcgcgatc agcatgcccg
     2641 acacggcgcc gatggccgag gccagcgtga aggtcagccc gcccgacatg gcggtgctga
     2701 tgcccaccag ccgcgcgccg cgccggttca ccgccgtggc gcgcagggcg cggccgtaca
     2761 gcgtcttgcc gaagaacagc cagagcgcca ggatgagcag caggcagacg cccaccacga
     2821 acaggctctg cgccgaccag ctcatgaagc ccaggttgac ctggccggac atgaaggtcg
     2881 gcgtgcgcca gccttcggcg ccgaagaaga tcaggcccag gccggtcagc gtgaaatgca
     2941 cggccaccga cacgatgagc agcaccagca ccgatgcctc ggccaggggg gtatagacaa
     3001 tgcggtacac catggggccc atgggcacca ccagcagcag gctcagcagc atgtcgaccc
     3061 acagcgaggt attgggaccg gcataggcgc gggtcagcca cagcagcagc aggggcagcg
     3121 ccacgcagct ccccagcacc gccggcaggc cggaccagat gccgctgcgc aaggcccgca
     3181 gcagctccag caacagcacc accagcccca gcatcggcag caggtacacc gtgcccggca
     3241 cccggccatc gaccagcatg gccagcgtca gggcgccgaa ggccacgaat tcgccctggg
     3301 gaatgaagat gacgcgcgtg acggcgaaca ccagcaccag agccatgccc agcagggcat
     3361 agatggcgcc gttgacgatg ccgtcctgca gcaggatcaa tgctatcgag aaatccacgt
     3421 ttcgatcttt tattcttttc tatatggaac acgccgcccg gatggggcgg cgtgcggcgg
     3481 cgactttaca gatcgggctg gtacacccat ttgccgccct cgaccttgac catgacgcgc
     3541 gaacgctcgt cgaagccggc gtggtcggtg gggctcatgt tgaacacgcc ttgcgaggcc
     3601 ggcaggttct tgacgccctc gatggcgtcg cgcatggccg cgcggaacgc cggcgtgccc
     3661 ggcttggcgc ccgtttgcag ggccaccggc agcgccgcca gaatcagttg gccggcatcc
     3721 cacacgtgac cgccgaaggg gttggtggaa cccgcgccgt aggcctgttc gtaggcctgc
     3781 acgaaggcca tggccgactt cttcaccggg ttgctgtcgg gcagctgcgc ggccaccagc
     3841 agcgggccgg ccggcagcag catgccttcg cagtccttgc cgcagacgcg cagcacgtcg
     3901 ttgttggccg cgccgtgggt ctggtagatg gtgccggcat agccgcgcgc tttcagttcc
     3961 ttctgcggca aggcggccgg cgtgccggcg ccggcgatca ggatggcgtc gggatgcgcc
     4021 gccaccagct tgagcgcctg gccggtgacg ccggtatcgg tgcggccata tttctcgacc
     4081 gccacgatct tgatgccctt ggcctcgccg gcctgctgca tcgcgttgag ccagctgtcg
     4141 ccgtaggcgt cggagaagcc gatgaaaccg agcgtcttga ccttggcctt ggccatggcg
     4201 tcagccaggg cgccggccat cagcgcgtcg ttctgcggcg tcttgaaagc ccagtggcgc
     4261 ttgtcgtcca tcggcgacac gatggccgcg ctggcggcca cgctgatcat cggcaccttg
     4321 agctcggcgg ccacgtcgac catggccagc gaacccggca cggccgaggt gccgatcagc
     4381 acgtccacct tgtcttcggt ggccagcttg cgcgcgttgc gcacggccgc ggtggggtcg
     4441 gtggcgtcgt cgagcacgat ccactcgatc ttctggccat ccgcctcctt gggcagcagg
     4501 gccgcggcgt tgcgctcggg gatgcccagc gaggcggcgg ggccggtcga ggaaagcgtc
     4561 accccgacct tgatctgggc attcgccgcg agcggcagcc ccaggaaaac cagggcggcc
     4621 gcggcggcgg acaggtgctt catcattatg tgttgtctcc ggaaaaaaaa cggcaatcct
     4681 accttacaaa tcaggctggt acacccactt gccgccctcg accttgacca tgacgtgcgc
     4741 gcgcgcgtcg aggccggcat ggtcggtggg gctcatgttg aacacgccct gcgaggccgc
     4801 aaggttcttg atgccctcga tggcgtcgcg catggccgcg cggaacgccg gcgtgcccgg
     4861 cttggcgccg gtcttcagcg cgacgggcag cgcgttgatg atcagttggc cggcatccca
     4921 catatggccg ccgaaggtat tgatagaacc ggcgccgttg gcctgttcgt aggcctgcac
     4981 gaaggccatg gccgacttct tcaccgggtt gctgtcgggc agctgcgcgg ccaccagcag
     5041 cgggccggcc ggcagcagca tgccctcgca gtccttgccg cagacgcgca gcacgtcgat
     5101 gttggccgcg ccgtgggtct ggtagatgag gccggtgtag ccgcgcgcct tgagttcctt
     5161 ctgcggcagg gccaccgggg tgccggcgcc ggcgatcagc acggcgtcgg ggtgcgccgc
     5221 caccaccttg agcacctggc cggtgacgct ggtgtcggtg cggctgtact tctcgacctc
     5281 gacgatattg atgcccttgg ccttggcggc ctgctgcatc acgctcagcc agccgtcgcc
     5341 gtaggcgtcg gagaagccga tgaagccgag cgtcttgacc ttggccttgt ccatggcatc
     5401 ggccagggcg ccggccatca gcgcgtcgtt ctgcggcgtc ttgaaggccc agcggtgctt
     5461 gtcgtcgacc ggttcgacga tctgggcgct ggcggccacg ctgatcaccg gtatcttgag
     5521 ctcggctgcc acgtcgacca tggccagcga accgggcgtg accgaggtgc ccaccagcac
     5581 gtcgaccttg tcgtccgtgg ccagcttgcg ggcattgcgc acggcggcgg tggtgtcggt
     5641 ggcgtcgtcg agcacgatcc attcgatctt ctggccggcg acttccttgg gcagcaggtt
     5701 ggccgcgttg cgctcgggga tgcccagcga ggcagccggg ccggtcgagg aaagcgtcac
     5761 gccgaccttg acctgggcaa ccgccgccaa gggcagggcc agaaccaggg cggccgctat
     5821 tttttgcaag cctgccttgc gcaggccgat acgggccgca gtgggcaaac tcgtgggcaa
     5881 gccaatacgc ttggtcatgt tttgtctcct gtttggctct tgattttgtg gccggatggc
     5941 caacgagcct gattaaagtt atacagaaaa tcgtatttta tgctgaaaac agtatcaaat
     6001 taaatcttcc tcagggttct tgaacaccgt atcaacccta ggttgggcgt gcaacccggc
     6061 gtaaccgggt ccggcgcgct gccgcctgcc acgggacgaa ggcccggcgg caatggatta
     6121 agataatcga ccctttccga atttcgccct ccggcctcgc cggatcttcg agagcctcat
     6181 ggaaaaagta cgcaagaccg acgccgaatg gcgcgccctg ctcaccccgc aggaattctc
     6241 cgtcgcccgc gaaaaagcca ccgagcgcgc tttcaccggc cgctactggg acaccttcac
     6301 cccgggcatc taccgctgcg tggcctgcgg cacggcgctg ttcgcctccg acaccaagtt
     6361 cgacgccggc tgcggctggc ccagctactt cgaaccgctc ggccccggcc tggtgcgcga
     6421 ggaaaaggac gccagccacg gcatggtccg caccgaggtg ctgtgcaata tctgcgactc
     6481 gcacctgggc cacgtcttcc ccgacgggcc gccgcccacg ggcctgcgct actgcatcaa
     6541 ttcgctgtcg ctgagcttcg aaccccagtc caaatgaaga agttcctgtt cgatctcttc
     6601 ccgctgatcc tgttcttcgt cgcctaccgc tacgccgaca tctacgcggc cacggcggtg
     6661 gcggtggttg catcaatcgc gcagatcgtc tggctcaagg ccaccagaaa gcccatcgag
     6721 gtcacgcact ggatcaacct gggcgtcatc gtgctgttcg gcggcgccac gctatggctt
     6781 cacagcgacg tcttcatcaa atggaagccg acggcgctgt actggctctt cggcgcggtg
     6841 ctgatcggcg gccggttcac cggccgcaac cccatgcgca agctgctggg ccagcagatc
     6901 gccatgtccg aggccgcctg gaacaggctc aacctcagct gggccttgtt tttcgcggcc
     6961 gcgggcctga tcaatctgta cgtcgccttc tcgggccatt tcaccgaggc gcaatgggtc
     7021 aacttcaagg tcttcggcct gatgggactg ctgctggtgt tcgtgatcgg ccagtcgatc
     7081 tggctgagcc gccatatgca gcccgacccg cagctcgacg aggccggccc ggccgacgag
     7141 gcgggcaaga acacgggcaa caccccatga cggtagaccg catcgcgctg atccgcgagc
     7201 gcctggccgc gctctcgccc gccagcctcg acatccagga cgactcccac ctgcacgccg
     7261 gccacgccgg cgcccagggc ggagccggcc actaccaggt ccgcatcgta gccagctgtt
     7321 tcaaggggct ctccgccgtg gcccgccatc ggctggtgta tgatcgtctg aacgatctta
     7381 tgccccatac catacatgcg ctggccatcg atgcccggcc gcctgtcgag acaacccccg
     7441 agacatccaa ggctcattga gccttcctct ctttcttccc ctagaaccat cgaggacttg
     7501 catttcatga aacgcatcat catgctggcc gccgcttgtg cgattgccgt gcccgccttc
     7561 gcgcagaacg tggctaccgt caatggcaag cccatctcac aaaagagcat ggacgatttc
     7621 gtcaaactgc tggtcaacca gggcgccacc gattcgcccc agctgcgcga ccaggtcaaa
     7681 gaagaaatga tcaaccgcca ggtcttcgtg caggccgccg agaagcaagg cctggccaag
     7741 caggcctcgg tgcagaccga agtcgaactg gcccgccagg gcatcctggt gcgcgccctc
     7801 atgaccgatt acctggccaa gcaccccgtc accgacgccg acgtccagac ccagtacgac
     7861 aaggtcaagc agcaggaatc gggcaagatg gagtacaagg tccgccacat cctggtcaag
     7921 gatgaaaaga ccgccgacga cctgctggcc cagatcaagg ccgacaagag caagttcgag
     7981 gacctggcca agaagaactc gcaagacccc ggcagctcgg gcaacggcgg cgacctgggc
     8041 tgggcccccg ccaccaatta cgtgcccccc ttcgccgacg ccgtcaccaa gctgaagaag
     8101 ggcgagatca ccgacaagcc ggtgcaaacg cagtacggct ggcacatcat catggtcgac
     8161 gacacccgcc cggtggagtt ccccccgctt gcgcaagtca agccgcagat cgaagaaatg
     8221 ctgcgccagc aaacgctggg cgactaccag aagtcgctgc gcgacgctgc caagatccag
     8281 taagcccctg cggtgcgaaa aagcccgctt catctcgtga agcgggcttt tttatttggc
     8341 acagacccga gttcttgata cgccggcgcc aggactgcgg gattccgatg ctcggcgcca
     8401 ctggcgcctg cgcttcggtt ccctccgccc tgccaccggc tcccatatcg tcacatgccc
     8461 ggtttgaaaa ccgtcgcatt gggctgacgg agccgaggac cccggagcaa tccgaagcgc
     8521 agcccccact aggggcgagc atcggaattg cgcagggcag cctcggcggc ccaagaactc
     8581 aaggcagtcg cgctcaaagc agccccaaca aggctttcaa ctcgtcttcg ctgagcacgg
     8641 ccacgcccaa ttcctgggcc ttggccagct tgctgccggc ctcctccccg gccacaacgt
     8701 aggccgtctt cttcgacacc gacccgctca ccttgccgcc cgccgccagg atatggcgcg
     8761 tggcctcgtc ccgcgaccag gtaggcatgg cgccggtgag cacgaacgtc ttgccggcca
     8821 gcgacgcgcc ctgcggctcg gcttcggcga ccggctcgac cccctgcccg cgcagctgcg
     8881 caatgatctc gcggttgtgc ggctcggcga agaagcggcg gatcgaaccc gccaccaccg
     8941 gccccacgtc gcgcacggcc agcaaggcct cttcgtcggc gtccatgatg gcctcgatgg
     9001 tgccgaaatg ccgcgccacg tcgcgcgccg tggtttcgcc cacgtggcgg atgcccagcg
     9061 cgaacagcag ccggcccagc gcaggctggc gcgcctgcgc gatcgcctgc accaggttct
     9121 cggccgactt ctcgcccatg cggtccagcg cggccagctc ctcggctttg agcccgtaca
     9181 gatcggccag cgatttcacc cggccgccgt cgaccagctg gtccaccagc ttctcgccca
     9241 gcccctcgat gtccagcgcc ttgcggccgg cggcgtgcag cagcgtctgc ctgcgctggg
     9301 cggcgcagaa caggcccccc gtgcagcgcg cgatggcctc gtcttccagc ttctcgatgg
     9361 ccgacccgca taccggacag gccgtcggca tgacgaacgg cgggggatcg cccacgcgct
     9421 tttccagcac ggggcccacc acttcgggga tcacgtcgcc cgcgcgccgc acgatcaccg
     9481 tgtcgcccac ccgcacgtcc ttgcggcgga cctcgtcttc gttgtgcagc gtggcattgg
     9541 tgaccgtcac cccgcccacg aacacgggct tcaggcgcgc cacgggcgtg atcgccccgg
     9601 tgcggcccac ctgcacctcg atgcccagca gctcggtcgt ggcctcctcg gccggaaact
     9661 tgtgggccac ggcaaagcgc ggcgcccgcg ccacgaagcc cagcacttgc tgcgcggcca
     9721 gggcgtcgac cttgtagacc acgccatcga tgtcgtaggg caggccggga cggatgcggc
     9781 ccacgtgctc gtagaaggcc agcagccccg ctgcgccctg ggcccggtgg ttgtgcttcg
     9841 cattgaccgg caggccgaac ccggccagcc agcccagcat gccgccgtgc gtatcgcgcg
     9901 gcaagccggc gtcttcgggg atctcgcccc agccgtaggc aaagaaccgc aggggccgct
     9961 gtgccgtgat gcgcggatcc agctggcgca ggctgcccgc ggccgcgttg cgcggattga
    10021 cgaagacttt ctcgccgcgc gccgcctggg cctggttcag gcgctcgaaa tcggcgcggt
    10081 tcatcagcac ctcgccgcgc acctccagca cgcgcggcgg cgcgcccagc aatcgcaagg
    10141 gaatggcgcg gatggtgcgg atattggacg tgacgtcctc gccctcctgg ccgtcgccgc
    10201 gcgtggcggc ctgcaccagt tcgccgtttt catagcgcag gctgatcgcc aggccgtcga
    10261 gcttgagctc gcagaagtag tccacctgcg cctcggcagc cagcaggccg gcgccgtgca
    10321 gcgtgtcggc cacgcgcttg tcgaaggcct ccacatcgtc ggactcgaag gcgttgccca
    10381 gcgacagcat gggcaccgcg tggcgcacgc tgccgaattc ggccagcggc gccgcgccga
    10441 cccgctgggt gggcgaatcg ggcgtcagca gttcgggatg ggccgcctcg agcgcctgca
    10501 gctcgcgcat cagcgcgtcg tagtcggcat ccgagatcga gggttcgtcg tggacgtaat
    10561 agcggacgtt gtgctcgt
//
LOCUS       DASWPO010000223         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2461422, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000223 DASWPO010000000
VERSION     DASWPO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461422"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGC69_11960"
     CDS             <1..891
                     /locus_tag="VGC69_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0296037.1"
                     /translation="GGSWRLFNALAAKGHFELITADLTDPRSLADALAQSPKLVLIET
                     PSNPLLRITDLRFVIDAAHKVGALAVVDNTFLSPALQKPIGLGADIVLHSTTKYVNGH
                     SDVVGGAVIARDAEVHQQLVWWANALGLTGSPFDAFLTLRGLRTLDARLRVHQENATA
                     IVGLLESHDAVAQVYYPGLPTHPGHAIAARQQTGFGAMISFELEGGEAAVRAFVDGLK
                     YFTLAESLGGVESLVAHPATMTHAAMTPEARAKAGISDGLLRLSVGIELAQDLVADLQ
                     AGLARAQAVQAKAERKLVDA"
     gene            888..1967
                     /locus_tag="VGC69_11965"
     CDS             888..1967
                     /locus_tag="VGC69_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296038.1"
                     /translation="MNAVVRLNARRAPRLALLGTGVVGSALVDRYQRLQARGIALPVF
                     AWVANSREVADGGQALGESLARLRKAPANTRIVPPWAEADALDAGDIVVDATASDAIA
                     LRHAEWLGRGVHVVTANKLGAGTDLLRAQAIDQAARDGGATYGDSATVGAGLPLLRSL
                     RALVAGGDTVRSVEGVLSGSLAWLFNQYDGTQPFSAYVRQARAAGYTEPDPRIDLSGE
                     DVRRKLLILARAAGFALQASDVRVASLVPQVLANAGDAQVDDLLSVLDAPLQETLEAA
                     RRDGAQLRVVGRFDAAGASVGLRVLPAGHVLCGGAGTDNRVAIHSDRYPDQPLLIQGP
                     GAGAEVTAAALLDDVLALLKDAPVA"
BASE COUNT          279 a          712 c          703 g          307 t
ORIGIN      
        1 ggcggcagct ggcgcctgtt caacgcgctg gcggccaagg gccacttcga gctgatcacc
       61 gccgacctga ccgacccgcg ctcgctggcc gatgcgctgg cgcaatcgcc caagctggtc
      121 ctgatcgaaa ccccgtccaa cccgctgctg cgcatcaccg atttgcgctt tgtcatcgac
      181 gcggcccaca aggtcggcgc gctggcggtg gtcgacaaca cgttcctgtc gccggcgctg
      241 cagaagccga tcggcctggg tgccgacatc gtgctgcact ccaccaccaa gtacgtcaac
      301 ggccatagcg atgtggtcgg cggtgcggtg atcgcacgcg atgccgaagt gcaccagcaa
      361 ctggtctggt gggccaacgc gctgggcctg accgggtcgc cgttcgatgc cttcctgacc
      421 ctgcgcggcc tgcgcacgct ggacgcgcgc ctgcgcgtgc accaggaaaa cgcgaccgcc
      481 atcgtcggcc tgctggagag ccacgacgcg gtcgcccagg tctactaccc gggcttgccg
      541 acccatccgg gccatgcgat cgccgcccgt cagcagacgg ggtttggcgc gatgatctcc
      601 ttcgagctgg agggcggcga agcggccgtg cgtgcgttcg tcgatggcct caagtacttc
      661 accctggccg aatcgctggg cggcgtggaa agcctggtcg cccatccggc caccatgacc
      721 cacgcggcca tgaccccgga agcccgcgcc aaggcgggca tctccgacgg cctgctgcgc
      781 ctgtcggtgg gcatcgaact ggcgcaggac ctggtcgccg acctgcaggc cggtctggcc
      841 cgtgcccagg cggtgcaggc caaggccgag cggaaactgg tcgacgcatg aacgcggtcg
      901 tccgactcaa cgcgcgccgc gcaccgcgcc tggccctgct gggcaccggg gtggtcggca
      961 gcgcgctggt ggaccgctac cagcgcctgc aggcgcgcgg catcgcgctg ccggtctttg
     1021 cctgggtcgc caactcgcgg gaagtcgccg acggtggcca ggcgctgggc gagagcctgg
     1081 cccgcctgcg caaggcaccg gccaacaccc gcatcgtccc gccctgggcc gaggccgatg
     1141 cgctggatgc cggcgacatc gtcgtcgatg ccactgccag cgatgcgatc gcgctgcgcc
     1201 atgccgaatg gctgggccgc ggcgtgcatg tggtgaccgc caacaagctt ggtgcgggta
     1261 ccgatctgct gcgtgcccag gccatcgacc aggctgcgcg cgacggcggt gcgacctacg
     1321 gcgacagcgc cacggtcggt gccggcctgc ccctgttgcg cagcctgcgt gcgctggtgg
     1381 ccggcggcga tacggtccgc tcggtcgaag gcgtgctgtc cggttcgctg gcctggctgt
     1441 tcaaccagta cgacgggaca cagccgtttt cggcctacgt ccgccaggcg cgcgcggctg
     1501 gctataccga gccggacccg cgcatcgacc tgtccggcga agacgtccgc cgcaagctac
     1561 tgatcctggc gcgtgcggcg gggttcgcgc ttcaggccag cgacgtgcgc gtggcctcgc
     1621 tggtgccgca agtgctggcc aacgccggcg atgcacaggt cgacgacctg ctcagcgtgc
     1681 tggacgcgcc gctgcaggaa accctggaag ccgcgcgccg cgatggcgcg cagttgcgcg
     1741 tggtcgggcg cttcgatgcc gctggtgcca gcgttggcct gcgtgtcctg ccggccggtc
     1801 atgtcctgtg cggcggcgcc ggcaccgaca accgggtcgc catccacagt gatcgctatc
     1861 cggaccagcc gctcttgatc cagggcccgg gtgccggtgc ggaagtcacc gccgccgcgc
     1921 tgctggacga cgtgctggcg ctgctgaagg acgctccagt cgcctagcag acgcggcaac
     1981 gaggattgtt gtccgcaagc t
//
LOCUS       DASWPO010000224         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3177072, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000224 DASWPO010000000
VERSION     DASWPO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3177072"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VGC69_11970"
     CDS             complement(<1..458)
                     /locus_tag="VGC69_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0296039.1"
                     /translation="MSTANSGPSYAAGPTDVPLIEQTIGDFFDAMAARQPQREALICR
                     HEGKRYTYLELQAEASRLASALLATGLAPGDRVGIWSHNNSAWVLMQLATAKAGLILV
                     NINPAYRLTEVEYVLNKVGCKALITATRFKTSDYIGMINTLAPELPIARPG"
     gene            709..1848
                     /locus_tag="VGC69_11975"
     CDS             709..1848
                     /locus_tag="VGC69_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0296040.1"
                     /translation="MPDKIPDRALQLRSLITSKGELEVSLSLADVPRPAADQVIVRIE
                     ASPINPSDIGLLFGPADITQARLEGTADTPVLKAPVAQAALGGIGGRLGQSLPVGNEG
                     AGVVVAAGDSPDAQALLGKMVSVAGGGLYAQYCLKNADDCLVLPPGTSAAAGASAFIN
                     PLTALGMLETMRREGHTALVHTAAASNLGQMLQNACLKDGIGLVNIVRSPEQADLLRQ
                     RGAVHVCDTSQASFMADLTDALEATGATIAFDAIGGGKLAGQILLAMEAAINRRGLPY
                     SRYGSDVHKQVYVYGGLNAGLVEIDRSAGMAWAVGGWLLYHFLKKIGREQTQWLRQRV
                     ASEIASTFKSAYAGNISLSEMVQPEIVAKYYKRATGEKYLVTPNA"
     gene            1995..2795
                     /locus_tag="VGC69_11980"
     CDS             1995..2795
                     /locus_tag="VGC69_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296041.1"
                     /translation="MASSSEIAQRIHHAVLASKLGAGDRLGEQSLAELFDCSRTVVRE
                     ALVELAVRGLVVSSARRGWYVVELTPEQTRQAFEAREVIEISILRHVRKVPAAAIRRL
                     REHVQRQEEAIRDDDAGLRSFLLGDFHVCLADSLGNTTLSDVLRDLTARTMLVAARHQ
                     SPRQAMCSCAEHAQIVHALAAGNMPLAEKRLAEHLRNWGGQLSAPEHADDPLDQLRQA
                     LEPVQPMAAATAARLRNANAAKSPRNSKSSKPAPKIPPRSPTDLGKLL"
     gene            2792..3604
                     /locus_tag="VGC69_11985"
     CDS             2792..3604
                     /locus_tag="VGC69_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296042.1"
                     /translation="MTSLRTPIKAVRTSLCLGLLACATLLASTAQAQTALDDILKAKE
                     IQIGIPTDFPPYGSMGTDMTPHGLDVDMAGYIASKLGVKIDLVPVSSNNRVAYLQTKK
                     VSLVISTLGKSPDREKVIDFTAAYSPFFIGVFGPKSIAVKTPADLAGKTIAVTTGAVD
                     DDELTKVAPSGANIRRFSENAGTVAAYSTGQTQLIAANIATASAIMSQNPQLNTEYKF
                     GLKDSPNFIGVAKGEDTLRMKVNEIIAQARQNGDLDKLSLKWLGKPAGDLPQ"
     gene            complement(3639..>4266)
                     /locus_tag="VGC69_11990"
     CDS             complement(3639..>4266)
                     /locus_tag="VGC69_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0296043.1"
                     /translation="VLSISETASGVLVKTAAGDYAAAKVIVTAGPWVPGLAGGRVAGN
                     LRVMRQVLHWFETTQPQRYHPSRCPVFIWMHGERDEDYLYGFPMVDGKDGVKVATEQY
                     DETCDPDAMNRLVSDDESQAMYARNVAGRLKYVEPRTVHRAACMYTVSSDSGFIVDRY
                     QDMENGLVVSACSGHGFKNSAGLGESLALKLMGEPTPGLAPFAVSRFA"
BASE COUNT          798 a         1449 c         1306 g          713 t
ORIGIN      
        1 cccggacggg cgatcggcag ttccggcgcc agcgtgttga tcatgccgat atagtcgctg
       61 gtcttgaacc gagttgcggt gatcagcgcc ttgcagccga ccttgttcag cacgtactcc
      121 acttcggtca gacggtaggc cggattgatg ttgaccagga tcagcccggc cttggccgtg
      181 gccagctgca tcagcaccca ggccgaattg ttgtgcgacc agatgcccac ccggtcgccg
      241 ggcgcgaggc ccgtggccag caaggcgctg gccaggcggc tggcctcggc ctgcagttcg
      301 agataggtgt agcgcttgcc ctcgtggcgg cagatcagcg cctcgcgctg gggctggcgg
      361 gcggccatgg catcgaaaaa gtcgccgatg gtctgctcga tcaacgggac gtcggtaggc
      421 cctgcggcgt aggacgggcc cgaattggcg gtgctcacga tagtctcctt caaggggggg
      481 ccagggcagt ctgcgttgcc ttctggcggg cttgaaaagt ctactccaca tcgtgcaccc
      541 gatgtccagt cgaaatcgtg ttcagcgcct tgcccggaat ggccatgacc agcccccggg
      601 tacacagcgg cgtggcatgg ctccactcag cactgaacga gacaggcggc acctatactg
      661 ctccgggaaa acctggcgtt cgccatgtcc aacttttcag gtgcccccat gcccgacaaa
      721 attccggatc gcgctttgca gctgcgctcg ctcattacca gcaagggcga gctcgaagta
      781 tcgctgtccc tggccgacgt accccgtccg gcggcagacc aagtcatcgt acgaatcgag
      841 gcctcgccga tcaatccttc cgacatcggc cttttattcg ggccggccga catcacccag
      901 gcccgcctcg aaggcacggc ggacacaccg gtcctgaaag cgcccgtcgc gcaggctgcc
      961 cttggcggga tcggcggccg cctcggccag tcgctgcccg tgggcaacga aggcgcgggc
     1021 gtggtcgttg cggcaggaga ttcccccgac gcccaggcgc tgctcggcaa gatggtatcc
     1081 gtggcgggcg gcggactcta tgcccagtat tgcttgaaaa atgcggacga ttgcctcgtc
     1141 ctgccgcccg gcaccagcgc cgcagccggc gcgtccgctt tcatcaatcc gctgacggca
     1201 ttgggcatgc tggagaccat gcggcgcgaa ggacacactg cgctggtcca caccgccgcg
     1261 gcctcgaatc tgggccagat gctgcaaaac gcgtgcctca aggatggcat cggcctggtc
     1321 aatatcgtgc gcagccccga gcaggcagac ttgctgcgcc agcggggcgc cgttcacgtg
     1381 tgcgacacca gccaggcaag cttcatggct gatctgaccg acgcgctgga agcgacgggg
     1441 gccaccattg ccttcgatgc cattggcggc ggcaagctgg ccggccagat cctcctggcg
     1501 atggaagcgg ccatcaatcg ccgggggcta ccctacagcc gctatggctc cgacgtgcac
     1561 aagcaggtct atgtctacgg cgggctcaat gcggggcttg tcgaaatcga tcgttccgcc
     1621 ggcatggcat gggccgtcgg cgggtggctc ctgtatcact tcctcaagaa gatcgggcgt
     1681 gaacagacgc aatggcttcg gcaacgggtg gcgtccgaga tcgcatccac gttcaagagc
     1741 gcttacgccg ggaatatctc gctatccgag atggttcagc cggagatcgt ggccaagtat
     1801 tacaagcgcg ccacgggcga gaagtatctc gtcaccccca atgcctgagt tcatccaggc
     1861 accgcggcgg ctggcttcat aagccgcaag gcctagggtt tgctctgtct tgtataccag
     1921 ttcagcttga tttgaaatac aagattgaat aaatctagca ttccagattt tctggaaaac
     1981 cctcgcgggc ccgcatggca agctcatccg aaatcgcgca acgcatccat cacgccgtgc
     2041 tggccagcaa gctgggagca ggcgaccgcc tgggcgaaca atcgctggcg gaacttttcg
     2101 actgcagccg cacggtggtg cgcgaggcgc tcgtcgaact ggccgtgcgc ggcctggtcg
     2161 tctccagcgc ccgccgcggc tggtatgtgg tcgaactcac gccggagcaa acgcgccagg
     2221 ccttcgaggc gcgcgaagtc atcgagatat cgatactgcg ccatgtccgc aaagtgcccg
     2281 ccgcggcgat ccgccgcctg cgcgagcacg tgcaacggca ggaagaggcc atccgcgatg
     2341 acgatgccgg cctgcgcagc ttcctgctgg gcgacttcca tgtctgcctg gcggacagcc
     2401 tgggcaatac cactttgtcc gacgtgctgc gcgacctgac ggccaggacc atgctcgtcg
     2461 cggcacgcca tcaatcgccg cgccaggcca tgtgctcctg cgccgagcac gcgcagatcg
     2521 tgcatgccct ggcggccggc aatatgcccc tggccgaaaa gcgtctggcc gaacacctgc
     2581 gcaactgggg cggccagctg tccgcccccg aacacgccga cgacccgctg gatcaattgc
     2641 gccaggccct ggagccggtg cagcccatgg cggcggccac ggccgcccga cttcgaaacg
     2701 cgaacgccgc caagtcgcca cgcaactcca aatccagcaa acccgctccc aaaattcccc
     2761 cgcgctcccc caccgaccta ggaaagctcc tatgacatcc ctacgcactc ccatcaaagc
     2821 tgtccggacc agcctctgcc tcggtctgct ggcttgcgcc acgctcctgg ccagcaccgc
     2881 ccaggcgcag accgcgctcg acgatattct caaggccaaa gagatccaga tcggcatccc
     2941 caccgacttc ccgccctacg gctcgatggg aaccgacatg acgccgcacg gcctggacgt
     3001 ggacatggcg ggctatatcg ccagcaagct cggcgtgaag atcgacctcg tgccggtgag
     3061 cagcaacaac cgcgtcgcct acctgcagac caagaaagtc tccttggtga tctccacgct
     3121 gggcaagagc cccgaccgcg aaaaggtgat cgacttcacg gcggcctatt cgcccttctt
     3181 catcggcgtg ttcgggccga aatcgatcgc ggtgaagacg cccgcggacc tggccggcaa
     3241 gaccatcgcc gtcacgaccg gcgcggtcga cgatgacgag ctgaccaagg tggcgccgtc
     3301 cggcgccaat attcgccgct tctcggagaa cgccggcacc gtggccgcct attcgaccgg
     3361 ccagacgcag ttgatcgccg ccaacatcgc cacggccagc gccatcatgt cgcagaatcc
     3421 gcagctgaac acggagtaca aattcggcct caaggattcg cccaacttca tcggcgtggc
     3481 caagggcgaa gacacgctgc gcatgaaggt caacgagatc atcgcgcagg cgcgccagaa
     3541 cggcgacctc gacaagctct cgctcaagtg gctgggcaag cccgccggcg acttgcctca
     3601 ataaccgatc ctggccggcc tgtggacacc aagccggctc aagcgaaccg gctgacggcg
     3661 aaaggcgcca ggccgggcgt cggctcgccc atcagcttca aggccagact ttcgcccagc
     3721 ccggcggaat tcttgaatcc atggccggaa caggcggaaa ccaccaggcc gttctccatg
     3781 tcctggtagc ggtcaacgat gaagcccgaa tccgagctga cggtgtacat gcaggccgcg
     3841 cgatgcacgg tgcgcggctc aacgtatttc agccgcccgg cgacattgcg cgcgtacatg
     3901 gcttgcgatt cgtcatccga cacgagccgg ttcatggcat ctggatcgca cgtttcgtcg
     3961 tactgttcgg tggcgacttt cacgccgtcc ttgccgtcca ccatgggaaa gccgtagagg
     4021 taatcctcgt cgcgctcgcc gtgcatccag atgaagaccg gacagcgcga gggatggtag
     4081 cgctgcggct gcgtggtttc aaaccagtgc agcacctggc gcatcacgcg cagattgccg
     4141 gccacgcggc ctcccgccag ccctggcacc cagggcccgg ccgtcacgat caccttggcc
     4201 gcggcatagt cgcccgccgc cgtcttcacc aacacgcccg aggcggtctc cgaaatcgac
     4261 agcact
//
LOCUS       DASWPO010000225         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1033733, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000225 DASWPO010000000
VERSION     DASWPO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033733"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VGC69_11995"
     CDS             complement(<1..970)
                     /locus_tag="VGC69_11995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY0296044.1"
                     /translation="MAQPDQLELSFDSGPAEPAGRPAAAVPAPRPAPAADAPLLVLTP
                     SPAHATPAARVPTPCPAKLPDGTRWREVDTPLQAIGFVLQRSRRRSIGFVIADDGLRV
                     TAPNWVTLGQIDEAVREKAKWILAKLREWQDRKEQLALTSTRWDAGGELPYLGKRIVL
                     GLAADRRQAFFAAAPSQGKNALLVGRQAEGAAPERVLAGDADDPQDGDTLWLPLSPGA
                     GQARVRDTAQAWLQQRASDRFGARLAHFLQLTGLTIRRWRLSSAATRWGSCTSDGSIM
                     LNWRLIHFAPDIIDYVIAHELAHLREMNHSQDFWAEVGHILPGFEEA"
     gene            complement(987..1697)
                     /locus_tag="VGC69_12000"
     CDS             complement(987..1697)
                     /locus_tag="VGC69_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0296045.1"
                     /translation="MAWLRSLIYAVYLTVTVIPYAFACLIWSPLPRAWRYRLTMGWPR
                     LAIWGARAICGIRWQIKGWENLPDGPAVLLSKHQSAWETLFLPSHMPRQICFVYKREL
                     HRVPFFGWGLGLLAMIPIDRSKGRDAFEQVVRVGQQRLAEGRWPLLFPEGTRVPPGKM
                     GRFKMGGAMLAARAGVPVVPIALNSGAYWRRNAFVKRPGLITVSIGPAIESQGLSPDE
                     LNAKVQAWIAGEMRVLET"
     gene            complement(1799..2335)
                     /gene="gmhB"
                     /locus_tag="VGC69_12005"
                     /pseudo
     CDS             complement(1799..2335)
                     /gene="gmhB"
                     /locus_tag="VGC69_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
     gene            complement(2364..>2878)
                     /locus_tag="VGC69_12010"
     CDS             complement(2364..>2878)
                     /locus_tag="VGC69_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEY0296046.1"
                     /translation="SAALADFIYDRLAGALREQGYSAQEIDAVLALRPQRLGEVPKRL
                     AAVRAFAALPEAAALAAANKRVGNILKKAEGQARERIDAALLKEPAEQALAEVLATVR
                     AASGQLFDSGEYAASLQELAALKGPVDAFFDNVMVNAEDPALRANRLGLLATLHAAMN
                     RVADLSRLAA"
BASE COUNT          405 a          925 c         1120 g          428 t
ORIGIN      
        1 tggcttcttc gaagccgggc aggatgtggc cgacctcggc ccagaagtcc tggctgtggt
       61 tcatctcgcg cagatgggcc agctcgtgcg cgatgacgta gtcgatgatg tcgggcgcga
      121 agtggatcag gcgccagttg agcatgatgc tgccgtcgct ggtgcaggag ccccagcgcg
      181 tggcggccga ggacaggcgc cagcggcgga tggtcaggcc ggtgagttgc aggaaatgcg
      241 ccaggcgcgc gccgaagcgg tcgctggcgc gttgctgcag ccaggcctgc gcggtatcgc
      301 gcacgcgggc ctggccggcg cccggcgaca ggggcagcca gagcgtgtcg ccgtcttgcg
      361 ggtcgtcggc atcgccggcc agcacgcgct ccggtgccgc gccttcggct tgccgcccca
      421 cgaggagggc gtttttgcct tgcgacggcg cggcggcgaa aaaggcctgg cggcgatcgg
      481 cggccaggcc cagcacgatg cgcttgccca ggtagggcag ctcgccgcct gcgtcccagc
      541 gggtgctggt gagcgcgagc tgttccttgc ggtcctgcca ttcgcgcagc ttggcgagga
      601 tccacttggc tttttcacgc acggcttcgt cgatctgccc cagggtgacc cagttgggcg
      661 ccgtgacgcg caggccgtcg tcggcgatga cgaagccgat gctgcgccgg cgcgagcgct
      721 gcaggacgaa gccgatggcc tgcagggggg tgtcgacttc ccgccagcgc gtgccgtcgg
      781 gcagcttggc ggggcagggc gtaggcaccc gggccgccgg ggtggcgtgg gcgggagagg
      841 gcgtgaggac cagcagcggc gcatcggcgg ccggcgccgg ccgcggcgcg ggcacggcgg
      901 cggcaggcct gccggccggt tcggcggggc cggaatcgaa cgacagctcg agctgatcag
      961 gttgggccat ggcgttcggg acgggtttac gtttccagca cgcgcatctc gccggcgatc
     1021 caggcctgta ccttggcatt gagctcgtcg ggcgagagac cctgcgattc tatcgcggga
     1081 ccgatggaga cggtgatcag gccgggacgc ttgacgaagg cgttgcggcg ccagtatgcg
     1141 ccggagttga gcgcgatcgg aacgacgggc acgccagccc gggcggccag catggcgccg
     1201 cccatcttga agcgccccat ctttcctggc ggcacgcgcg tgccctcggg aaagagcagc
     1261 ggccagcggc cctcggccag gcgctgctgg ccgacgcgca ccacctgttc gaaggcgtcg
     1321 cgccccttgc tgcgatcgat ggggatcatg gccaggagcc ccaggcccca gccgaagaag
     1381 ggcacgcgat gcagttcgcg cttgtagacg aagcagatct ggcgcggcat gtgggacggc
     1441 aggaacaggg tttcccaggc cgactggtgc ttggacagca gcacggccgg gccgtcgggc
     1501 aggttttccc agcccttgat ctgccagcgt atgccgcaga tggcgcgcgc gccccagatg
     1561 gccaggcggg gccagcccat ggtgagccgg tagcgccatg cgcgcggcag cggcgaccag
     1621 atcaggcagg caaaggcata gggaatgacg gtgacggtga ggtaaacggc gtagatgagg
     1681 gagcgcagcc aggccatcca ggtcaacctt ttttagaaga agggtcgtcc gccgccgggc
     1741 tgtccgaagc cggacgcgcg ctttcggggg gtggcacgcg cgcggcgcgc ggcgcggggg
     1801 cctccgacag gatcctttcc gcgaaggccg gcagcccgtc gcagatctgc gtgcccggcg
     1861 gcaggccgcc cttgtcgcgc gtcttgacgc cattgccggt gagcgccagc cagggcgtgc
     1921 agcccgcggc ggcggcggcc tgcaggtcgc gcaacgagtc gcccagcgcc ggcacgccgg
     1981 ctaggtccac gtcatagcgg cgggcgatgt cgaggtacat gccgggcagc ggcttgcggc
     2041 aggtgcagcc ttcgtcgggg ccgtgcgggc acaggaagac ggcgtcgatg acgccgccca
     2101 gctgggccag ttcctggtgc atcttcgagt ggatggcgtt gagcgccgtc acgtcgaaca
     2161 ggccgcgcgc caggccggac tggttggtgg ccaccaccac ggtccaaccg gcctgcgaca
     2221 ggcgcgcgat ggcctgcagg ctgcccggca gggccaccca ttcgtcggga tttttgacga
     2281 aggcgtcgct gtcctgattg atgacgccgt cgcggtcgag gatgacgagt ttcatagggg
     2341 ctctaggcgg gctttggata gggctaggcg gccaggcgcg acaggtcggc cacgcggttc
     2401 atcgccgcgt gcagggtggc gagcaggccc agccggttgg cgcgcagcgc cgggtcttcg
     2461 gcattgacca tcacgttgtc gaagaaggcg tcgaccgggc ccttgagcgc ggcgagctcc
     2521 tgcagcgagg cggcgtactc gccgctgtcg aacaattggc cggaggcggc gcgcaccgtg
     2581 gccagcacct cggcgagcgc ctgctcggcg ggttccttga gcagcgcggc gtcgatgcgc
     2641 tcgcgcgcct ggccctcggc ctttttcagg atgttgccga cgcgcttgtt ggccgcggcc
     2701 agcgccgcgg cctcgggcag cgcggcgaag gcgcgcacgg cggccaggcg cttgggcact
     2761 tcgcccaggc gttgcgggcg caaggcgagc acggcgtcga tttcctgcgc gctatagccc
     2821 tgttcgcgca gggcgccggc gaggcggtcg tagatgaagt cggccagcgc ggcgctcg
//
LOCUS       DASWPO010000226         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_856125, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000226 DASWPO010000000
VERSION     DASWPO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856125"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VGC69_12015"
     CDS             complement(<1..315)
                     /locus_tag="VGC69_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HEY0296047.1"
                     /translation="MSFPDISPGLLYLTASVGCSVVVSVLLKFMRSAGVDLRQAIFSN
                     YIVAAVLCWLIFVPAPVQSLERIHAWPLMLTLAVLLPAVFVAMGQSVRHAGIVRSDVA
                     QRL"
     gene            complement(620..802)
                     /locus_tag="VGC69_12020"
     CDS             complement(620..802)
                     /locus_tag="VGC69_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296048.1"
                     /translation="MSFITAVLLFVLFIWPLGRILRRTGHHGWWAILSIIPVVNVLAI
                     WYWAYKDWPPSKVTAS"
     gene            complement(907..1746)
                     /locus_tag="VGC69_12025"
     CDS             complement(907..1746)
                     /locus_tag="VGC69_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0296049.1"
                     /translation="MNRQAISKTRQSASDRWSEFVDSLVAFDPDVLDGAAVALRVEAT
                     RASHDLPMHSHRRGQLVMATHGSVRCDVADGFWTIPPRCALWIPAGHPHRSQVSAKGR
                     IIFLYIAPGSISLPDACCTLLITPLVEELIRHLAAQKQDYDPSSPTARLVDVLLEQLQ
                     EMPTTRLHLPVPKNPRLAKIADALFTDPANRNTVAQWAASVALTERTLARLIHAELGM
                     SFGRWRQQLHIIVALQQLAEGTAVQSVSQQLGYESVSAFITMFKKMMGHPPGHYLQRA
                     DSA"
BASE COUNT          406 a          563 c          616 g          356 t
ORIGIN      
        1 aagccgctgc gccacgtcgc tgcgcacgat gccggcatgg cgcaccgact gccccatggc
       61 cacgaacacg gccggcagca gcacggccag cgtcagcatc agcggccagg catggatgcg
      121 ttccagcgac tgcacgggcg cgggcacgaa aatcagccag cacagcacgg cggccacgat
      181 gtagttgctg aagatcgcct ggcgcaggtc gacgccggcg ctgcgcatga acttcagcag
      241 caccgagacg accacgctgc agcccacgct ggcagtcagg tagagcaggc cggggctgat
      301 gtcgggaaag ctcatgcggg gtgactcctg ggtaatgtgc cggggcgccg gggctgagag
      361 gccatggttg gaggcgaatt gccgggatcg taaggacgtc cggcaagatg cgcaatggca
      421 gcaagtgact gaaagatgag ggtagtttgg taggacttgt ctgacaaagc ctgattttga
      481 ataaagtgag ccggtatcgt gccgggattt cctgccagcc cgcaagccgc gaatttatgc
      541 atttttttgt aaatcaccgc cacggcgcat gaaaaagccg cctcttgggc ggcttgtgct
      601 gtcgcgcagg cgggcggcct tacgacgccg tgactttcga cggcggccag tctttatagg
      661 cccaatacca gatcgccagg acgttgacga cgggaatgat gctgagaatg gcccaccatc
      721 catgatgccc cgtcctgcgc aggatgcgtc ccaaaggcca gatgaacaag acgaaaagaa
      781 gaaccgcggt gatgaaagac atagcgtgtt cctgatgaga gaaaaatttt tggaaacgtc
      841 gatatctcgg tactgcgctt atcactatac gcacgccatg gcgccggcgc aggcaaattc
      901 atccgatcaa gcgctgtccg cgcgctggag ataatgcccg ggcggatggc ccatcatctt
      961 cttgaacatc gtgatgaagg cgctgaccga ctcgtagccc agttgctggg agaccgattg
     1021 cacggccgtg ccttcggcaa gctgttgcag cgcgacgatg atgtgcagtt gctggcgcca
     1081 ccgcccaaag ctcattccga gttcggcatg gataagcctc gcgagcgtac gctccgtcag
     1141 cgcgaccgaa gccgcccatt gggccacggt attccggttc gccggatccg tgaacagtgc
     1201 gtccgcgatc ttcgcgagcc tggggttctt gggaaccggc agatgcagcc gtgtcgtcgg
     1261 catttcctga agctgctcga gcaagacatc caccagcctc gccgtgggac tggacgggtc
     1321 gtagtcctgc ttttgcgccg ccagatgccg gatgagctct tccaccaatg gggtgatcaa
     1381 cagggtgcag caggcatcgg gcaaggagat gcttcccggc gcgatataga gaaaaatgat
     1441 gcgtcccttg gcggatacct ggcttcgatg cggatgcccc gccgggatcc atagcgcgca
     1501 gcgcggcggg atcgtccaga agccgtcggc gacgtcgcac ctgaccgagc cgtgcgtcgc
     1561 catgaccagc tggccccgcc gatgcgagtg catcggcagg tcgtggcttg cccgcgtggc
     1621 ctcgacgcgc aatgcgaccg ccgccccatc caggacgtcc ggatcgaagg ccaccaggct
     1681 atcgacaaac tcggaccacc ggtcggaagc agattgtctg gttttggaaa tcgcttgtct
     1741 gttcacggaa atattcccgt attggtcagg cttatagtga tttcaaggct cgccggtctt
     1801 ccaggcgagt tccaacccga actgccgagg ggggcgtatg acgacaatga gtatacctat
     1861 caccgcaacg gatggatgcc aggatccggt gatgctgaac gaatggcacg caataggtta
     1921 ctcaacggat ttcgtgccgg a
//
LOCUS       DASWPO010000227        17774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2015970, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000227 DASWPO010000000
VERSION     DASWPO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 17774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17774
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015970"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VGC69_12030"
     CDS             complement(<1..1264)
                     /locus_tag="VGC69_12030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0296050.1"
                     /translation="MKRNTMAVGLIFQPMGNHPASWLLSDAPAGAEVSLDHYVNIARI
                     AESGALDFMFFADLPAIRDGNMNAIRRWPLYVSQFEPVTLLGALAASTRRLGLAATVS
                     TSFYEPYNLARQFASLDHLSKGRVGWNVVTTSSGAAAPNFGDKGREDHASRYARAREF
                     LTIVKGLWDSWEDDAFLRDKESGIFFDPGKLHRLDFNGKHFAVRGPLNVPRPPQGHPV
                     IIQAGGSEAGKELAAETAEVVFTADRNIDNAKKFYADLKGRMPRFNRALDQLKILAGL
                     NPIIGATKTEAEDKFDRLQRHIHPDVGREILSIDLDGVDLSDVPLDSPIPDDKIPKDI
                     EGGKSYLGYIRKLMNEGPVTLRQLYEIYAPARGGNFIVGTPKDIADMMEEWFVGKAAD
                     GFMIGLAHVPSDAQAFVEGVVLALRKRGL"
     gene            complement(1277..2377)
                     /locus_tag="VGC69_12035"
     CDS             complement(1277..2377)
                     /locus_tag="VGC69_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296051.1"
                     /translation="MNEANRLGETRKADKAELKGIAKSFGAKVAVQVDALSIAEGEFL
                     TLLGPSGCGKTTLLMMLAGFIQPDRGEIRAGMRDITRLPPEQRNFGMVFQGYALFPHL
                     SVRDNIAYPLRRRGTDAAALRKRVDAMLELVQLPHLADRMPKQLSGGQQQRVALARAL
                     VFEPDIVLLDEPLSALDANLRHDLQGELKAIHQETGRTFVCVTHDQDEALSMSDRIAV
                     MRDGHIIQLADPYTLYNRPASRFVAKFMGAKNFLDMKVESVEGGVAVCRVNGVRVRQA
                     LGGAAAAAGAEITAALRPGAIRLGAGERNTVQGVVVSCNYTGDGFVVTLDTDIGPLTL
                     HHPASLASDAISVGARVQASWHDDAPVLVNND"
     gene            2505..3401
                     /locus_tag="VGC69_12040"
     CDS             2505..3401
                     /locus_tag="VGC69_12040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296052.1"
                     /translation="MADAIAAARVQAPYSRPGWMAWLTSHKEFLVLSVPLVLALVVFF
                     ILPLLNVFLLSVTEPSTGLGNYALLVSSEGIRHILWRTVRLCAVTTVFSVAAAYLVAY
                     VLAASTPRQRSLMMFFILVPFWVSALVRAFAWLLLLGQDGPVNQALVGLGVMARPFQF
                     LFNEVGVTLGMVHYMLPYAVLPLYALMKDIDPQLIRASRGLGATAFGAFIHTYLPLTV
                     PAIVASSSLVLVYSLGFYIIPAILGGGRVLMIAQYISINVLETVRWGLASMLSVTLLV
                     SVLLLSLATGRLGRRATQGGNA"
     gene            3401..4192
                     /locus_tag="VGC69_12045"
     CDS             3401..4192
                     /locus_tag="VGC69_12045"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296053.1"
                     /translation="MDQALSGWRFRGLQAGAWVVLAFLFLPLLVVIPVSLSNQSYLSL
                     PRHGLSLQHYDKVFGDPAWRASIGLTAALSAVSALIATVLAGMAAIACWRFRGPGAWA
                     LRALVMAPLVVPSIVSALGFYQMWAKLGLLDTYAGVVIANVVQTIPYAFTAVSASLLL
                     LDPQLEKAARNLGATQFGAIWTVVVPATRAGIFSGMLFAFVYAWDEVVVQLFITSRGV
                     RLLPRLIWEGLQDTVDPAIAVIASCLTVLTAVLILAMGWAQNRKK"
     gene            4293..5357
                     /locus_tag="VGC69_12050"
     CDS             4293..5357
                     /locus_tag="VGC69_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296054.1"
                     /translation="MTISKNGGVNRRDVLKLGTALAVSPLLAGTARAQSAPITLVNWG
                     GDSLKGMTDAFAAPFEKATGIKVQVDTSGPSAGKIRAMVQSGKVTWDVCDGTVGYCLE
                     LGPAGLLEEIDYAIVPKTATLDSFAFKWGVGSYAYSSVLAFDKEKFKDKAPSNWADFW
                     NLKDFPGRRLLRADLPTMLEAALMADGVDPAKLYPLDTKRGFEKLREIKENTIFWKTA
                     AESAQLLRNGEVVMGNIWSTRAAPLSMEPNSKIGFTWNQGILQSAVWIVPKGNPAGRE
                     AVMKLIAFMQQPEPAIALSRVLYCGPMSPAAAAALPADLRPLVPTAPENVKVQVPMDG
                     MWYAENYQKELPKYLDLIAS"
     gene            5419..5931
                     /locus_tag="VGC69_12055"
     CDS             5419..5931
                     /locus_tag="VGC69_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0296055.1"
                     /translation="MIDVDEFRRLFQAQVSSVAVITAQAGERRAGFTATAVCSLQDDP
                     PSLIVSINRKTNAYPLIVESKRFAVNFLAADQEHVSARFAKHAADLDEAAMKFDAAGD
                     WCLSAHGALLLQNATAVAECELVQSVELNTHAILIGNILDAEVSETAKPLLYGFKRYV
                     RIPETREQHA"
     gene            5934..7373
                     /locus_tag="VGC69_12060"
     CDS             5934..7373
                     /locus_tag="VGC69_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0296056.1"
                     /translation="MKDSMLLSIDGLRKAYAGKAVSPVEVMRHTIGQINALNPELNFV
                     YEMHEEEALRMAAESERRWMAGRPAGLLDGIPATIKDALPLAGYCSYRGTAAEDPDPV
                     PSTTDAPCVARLKEEGAIAFGKTTMCDLGILPSGTGSSYGVTRNPWNPARNPGGSSAG
                     AAASVAAAVHPFAIGTDIVGSIRLPAAWSGIYGFKPSQGRVPYYFPNSPSLVAGPMTH
                     SVRDAAELMNIIARPDPRDFAALAYDGFDYLAQLDAPIKPGRIGIVRDLGFDAKPDTE
                     SLGVLDAAAAILKSMGWEIRDIAPPCTQQDLLAAENFYRVRAYAELKTKPMARREKAA
                     VVNGWASAAASASASDFYDWFNTLQRCREQVVSMMRYVDYLLLPPTLTAAPEAEFPRS
                     AADALFVPWAQTYLFNLSEQPAASVPMRLSGAGLPLGVQIVGRRFDDAGVLRLSARLE
                     AARGRFPLSPYAQRGIQGFNERLNEIARG"
     gene            7376..8515
                     /locus_tag="VGC69_12065"
     CDS             7376..8515
                     /locus_tag="VGC69_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0296057.1"
                     /translation="MRFTLLVIPYLRERGEKVGAVGGEPARFQRLMQQLKTQMVFAES
                     VGYDGFCMTEHHLQVEGVESTTNPLFWDYFVAQHTKKMRVGQLGMNLTVINPIQLAEN
                     IAMLDHLTGGRVFAGFSRGNTARWANTFGQHIGIGSTESDRSAADQRNREIFYENWNI
                     VKQLWTQPTVDIDGKYWQVPPEMHWHFAPTDDWAPETLGPDHALKRVGIVPRPLQTPH
                     PPVYAPFSYSMETSKFWAREGGKMVSFVAPEKESFIRTALDVYMAEAEQAGRQTTTQD
                     SLAIGGHLVCGSTPAESRIIEDGFRELFRYAYDAPPYHVPMGRLWKGSRQQMLDDVAT
                     LAEKFGVEEFFCWHHVGWFTQEQEMSALNEFAEAVIKPLNGEVKL"
     gene            8528..10084
                     /locus_tag="VGC69_12070"
     CDS             8528..10084
                     /locus_tag="VGC69_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0296058.1"
                     /translation="MLTLGDLCRRNAERWTNKDAYVEEERRVSWGEFDRRTDQLGAAL
                     RAMGVKPGDRVAMLGYDCVETAEVFMACAKIGAVRVGLNPRLAAREIAALMQDCGASI
                     LFVAASSEALVGEAAAAQPALPLIGFGAGHAQAIDYERLIATEHGIGRLPQTPSPVVM
                     IAYTTGSTGLPKGAVYEHGPFLQSIMMIALCEGVDSSSVWLHAMPAAGIPIMHMFRNI
                     FHGSTTVIVGPWDAQKALRLIRNERTTNTVLVPTMLNSLLACGDLGSTDTSSMRLLGY
                     GASTLPAATIRAAMQAFDCPFLQMYGTTELMGMGMMLFPSDHALGLSTYPEILTSTGK
                     ALPYVDVKVIDDDGNVLGAGEVGELIIYSEYRFKEYLNNPQQTADALRDGWIHTGDIG
                     QVSPEGYVFLHDRAKFKMKTGGYLVYPTEVENALAEHDAVDEVAVVGIPDERWGDRIE
                     ALITLKPSTRVDEAALLAFCKERIASFKVPKRIVISDLMPKGPTGKIQKLDVIKVLKQ
                     SAEQAGGAKA"
     gene            10081..10491
                     /locus_tag="VGC69_12075"
     CDS             10081..10491
                     /locus_tag="VGC69_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0296059.1"
                     /translation="MSGPAPEAGPQQRVVDGLRQGRLLLQKCRSTGEYLFYPRAAARG
                     VRSGDWDLVPACGLGRVYAATHVGMRGAPSYHIALVDLAEGPRLMARLEWPGDIPPPP
                     DTPVRMKMVEPEWAPQLGHPVPVFEPAGAGGGTP"
     gene            10488..11636
                     /locus_tag="VGC69_12080"
     CDS             10488..11636
                     /locus_tag="VGC69_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY0296060.1"
                     /translation="MTHPLRGAAAIVGQGYSQLGSVPGVSAMELLAQAAERAVEDAGL
                     DFSDIDGLCAGTFYHFWPTLSVAEYLRIEPAWSNADMVGGSSFMAHVQQAALAIQAGL
                     CHTVLIAYGSNARSLRDGAGLIETPWTERWSAPAIPIAGYALAATRHMHQYGTTRAHL
                     AEVAVAARKWAQRNPLATARDELALDDVLAAPQIATPFSKYDCCLVSDGAAAIVLTSA
                     DHAAHGRRKPVYVLGAASAHRHREIAQMGDLTSTAAVQSGRAAFAMAGVAPKDVDVVQ
                     VYDAFTLNTLLFLEDLGFCAKGEGGAFVEGGAIAPGGRLAVNTSGGGLSFVHPGAYGL
                     FGLSESVLQLRGETGARQVEGARLAVAHGNGGTLSHQSTVVLGTADYL"
     gene            11695..12075
                     /locus_tag="VGC69_12085"
     CDS             11695..12075
                     /locus_tag="VGC69_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0296061.1"
                     /translation="MQGQEQLLAFAQEYFEVLHTCDVVRAAKIFHPECGLFNVVEGKI
                     NFVPGRQYLEILKGRESPRSRGEPVYGKVLTIDQTDENTAFLKVLSSVQPRYFEDYLT
                     LLREDGQWRVVAKVYRALAACRDD"
     gene            12180..13523
                     /locus_tag="VGC69_12090"
     CDS             12180..13523
                     /locus_tag="VGC69_12090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0296062.1"
                     /translation="MSHGRMSIGLHFHPVGTHPASWLCSDAPAGAEVSIDYYAEVARI
                     AESGALDFMFFADVPAMRDGNMAASRRWPLYAAQFEPVTLLGALAASTRRLGLAATVS
                     TSFYEPYNLARQFASLDHLSKGRVAWNVVTTSSGAAAFNFGRNPRQEHAQRYERAREF
                     LAIVKGLWDSWDDDAFPRDKASGVFFDPGKLHRLDFNGKHFAVRGPLNVPRSPQGHPV
                     IIQAGGSETGKAFAAETAEVAFTTESSLAGAQKFYADLKGRLPGFMRTEDQLKILSGL
                     KPILGMTKTEAEDKFDRLQRHIHPDVGREILSIDLDGVDLSDVPLDSPIPDDKIPKDV
                     EGGKSYFGYLRKLMDNGPITVRQLYEIYAPARGGNFIVGTPKDIADMMEEWFVGKAAD
                     GFMIGLAYVPCDARLFVEHVVPALRRRGLFRLAYEGATLRDHLGLARPVSRYASR"
     gene            13656..15008
                     /locus_tag="VGC69_12095"
     CDS             13656..15008
                     /locus_tag="VGC69_12095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0296063.1"
                     /translation="MSARFMHIGALLSHVGIHPAAWLEPGVPLGGEVDLANWIGMAKT
                     AEQAKLDFIFRADSPASREGDLEAITRFPGHIAELEPLTIMSALAAVTKNIGLASTIS
                     TSFYEPYNVARLYASLDHISGGRAAWNVVTTSASIAAKNFGKTGLEDHAQRYARANEF
                     VDVVTGLWDSWDEDAFIRNRASGRFFDPSKRHVLDYKGEFYSVRGPLDVPRSPQGRPV
                     IVNAGGSEAGMELAARTAEVVFSVDRSLEKAKAFYANLKGRMAKYGRRPEALKIVCAL
                     NPYVGETDAQGQEKLDYLQSMIHPAVGREIISVDLGGIDLHNLPLDEPIPESALPTAS
                     NATKSYFDNMVAAIREDKLTVRQLYLACAASRGGMNAIAGSGATIADRMEQWFKEGAA
                     DGFMVRVSHLPGGLEDFTKYVIPELQERKLFRTEYSGPTLRDQLGLARPQSRYRPTSS
                     "
     gene            15022..16143
                     /locus_tag="VGC69_12100"
     CDS             15022..16143
                     /locus_tag="VGC69_12100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0296064.1"
                     /translation="MKKQVQQHLGVPAGTVQAKRRSVLKAAAAAGIFLPAMAGYQRRA
                     FAQTKVVNFASYGGSYGDALRKAWLDPFEKETGIKVNLGVNANLALAKLQTMNANGAE
                     WDIVDLSGQEYSIAAKENILLPLDHKLIDTAKLEPDFVKSHGFGYASYIWGMAWDRRK
                     IPDETAPKSWPEFWDKQKYPGKRSLIQVNTNGHAIEAALLADGVPVSKLFPLDVPRSL
                     KSLDKLGRDNIVWNTSNQEPVQRLTSGECPLAGMFVGRALMANRSGASIGFSLNGANI
                     APDYLGVIRTARNKPEAHALLNYIATRADRAAEFTTITSYPVPIQGLAALIPASAADI
                     LAVLPSDPKVKAQIYEDNGAYWAENLPRVAAMFKQWQLT"
     gene            16233..17057
                     /locus_tag="VGC69_12105"
     CDS             16233..17057
                     /locus_tag="VGC69_12105"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296065.1"
                     /translation="MQRKYNLAQSFLLTPVVLLYGLAAVAPLLVIIELSLQKGLATYL
                     EVIASPLFFKTAINTLAISIETTVAAVVLGYLVSALLVQAKGGWRVLIMAMVLLPFWT
                     SALIKNFAWAALLQDNGVINGLLMRLGLVTQPLTLLHTRFAVVVGMVHYVIPYAVFPI
                     YSAMLAIDPALYRAAQSMGASRLSSSTHITLPLTRPGLYAAFLLVFIISIGFYITPIV
                     LGSPRDMMVANLVDFYTHQLIDFSSASALAIVILVVTAFFIALYNSIPKEGQYGKI"
     gene            17044..>17774
                     /locus_tag="VGC69_12110"
     CDS             17044..>17774
                     /locus_tag="VGC69_12110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296066.1"
                     /translation="MARSNTLLLIACLPIFVFLLGPILIIVPMSLTQGDILVFPPSGV
                     SLHSFFDLFMDPQWVSSGVTSFKVALLATLISTLVGTASALALHRNAVPGRGALSTLI
                     MLPLLMPVIVLALGDYLFSSRIDLSSKWVAIAIAHSVVVTPYVFVSVQTSLAGLDPAL
                     HRAARSLGGDSWALLAHVYWPAIRSGVLGGALFSFIGSFDEVVISLFLSGPDAVTLPV
                     QMFTAIQYDLTPKIAAVSTVLLAISV"
BASE COUNT         2983 a         5737 c         5884 g         3170 t
ORIGIN      
        1 acagcccgcg cttgcgcagc gccagcacga cgccctcgac gaaggcctgg gcgtcgctcg
       61 gcacgtgcgc caggccgatc atgaagccgt ccgcggcctt gccgacgaac cactcctcca
      121 tcatgtcggc gatgtctttc ggcgtgccca cgatgaagtt gccgccgcgc gccggcgcat
      181 agatttcgta cagctggcgc aaggtgaccg ggccttcgtt catcagcttg cggatatagc
      241 ccagatagga cttgccgccc tcgatgtcct tggggatctt gtcgtcgggg atggggctgt
      301 cgagcggcac gtcggacaag tccacgccgt ccaggtcgat cgacaggatc tcgcggccca
      361 cgtcggggtg gatatggcgc tgcagcctgt cgaacttgtc ttccgcttcc gtcttggtcg
      421 cgccgatgat ggggttcagg cccgccagga tcttcagctg gtcgagcgcg cggttgaagc
      481 gcggcatgcg gcctttcagg tcggcgtaga acttcttggc gttgtcgatg ttgcggtcgg
      541 cggtgaagac gacctcggcg gtttcggcgg ccagctcctt gccggcttcc gagccgccgg
      601 cctggatgat gacgggatgg ccctgcggcg ggcgcggcac gttcagcggg ccgcgcaccg
      661 caaagtgctt gccgttgaag tccaggcggt gcagcttgcc gggatcgaag aagatgccgg
      721 attccttgtc gcgcaggaag gcgtcgtctt cccagctgtc ccacagcccc ttgacgatgg
      781 tcaggaactc gcgcgcacgc gcgtagcggc tggcatggtc ttcgcgtccc ttgtcgccga
      841 agttgggcgc ggccgcgcca gacgacgtgg tgacgacgtt ccagcccacg cggcccttgc
      901 tcaggtggtc gagcgaggcg aactggcggg ccaggttgta cggctcatag aacgaggtgg
      961 agacggtcgc ggccaggccg agccggcggg tcgacgcggc cagcgcgccc agcagcgtca
     1021 cgggctcgaa ctgcgagacg tagagcggcc agcggcggat ggcattcata ttgccgtcgc
     1081 ggatggcggg caagtcggcg aagaacatga aatcgagcgc gcccgactcg gcgatgcgcg
     1141 cgatgttcac gtaatgatca aggctgactt ccgcgcccgc gggcgcgtcg gagagcagcc
     1201 aggacgcggg gtggttgccc atgggttgga aaatcaggcc aacggccatc gtgttgcgtt
     1261 tcatgtgtcc catctcttag tcgttgttga cgagcaccgg cgcgtcgtca tgccatgagg
     1321 cctgtacgcg ggctcccacg gaaatggcat cgctcgccag gctggcgggg tggtggaggg
     1381 tcaaggggcc gatgtcggtg tcgagggtga cgacgaatcc gtcgccggtg tagttgcagg
     1441 agacgacgac gccttgcacc gtgttgcgtt caccggcgcc caggcggatg gcgccgggcc
     1501 gcaaggccgc cgtgatctcg gccccggccg cggctgccgc gccgcccagg gcctggcgca
     1561 cgcgcacgcc gttgacgcgg cagacggcga cgccgccttc gacgctttcg accttcatgt
     1621 cgaggaagtt cttggcgccc atgaacttgg ccacgaagcg gctggcgggg cggttgtaga
     1681 gcgtgtaggg gtcggccagc tggatgatgt ggccatcgcg catgacggcg atgcggtccg
     1741 acatggacaa ggcctcgtcc tggtcgtggg tgacgcagac gaaggtgcgg ccggtttcct
     1801 ggtggatggc cttcagttcg ccttgcagat cgtgccgcag gttggcgtcc agcgcggaca
     1861 gcggctcgtc gagcaggacg atgtcgggct cgaagaccag cgcgcgggcc agggcgacgc
     1921 gctgctgctg cccccccgag agctgcttag gcatgcggtc ggccaagtgg ggcagctgca
     1981 cgagttcgag catcgcgtcg acacgcttgc gcagcgcggc ggcgtccgtg ccgcggcggc
     2041 gcaagggata ggcgatgttg tcgcgcacgc tcaagtgcgg aaagagggca tagccctgga
     2101 acaccatgcc gaagttgcgc tgctccggcg gcaggcgcgt gatgtcgcgc atgccggccc
     2161 ggatctcgcc gcggtcgggc tggatgaacc cggccagcat catgagcaag gtcgtcttgc
     2221 cgcagcccga gggcccgagc agcgtcagga attcaccctc ggcgatggac agcgcgtcga
     2281 cctggacggc caccttggcg ccgaagcttt tggcgatgcc cttcagttcc gccttgtctg
     2341 ccttacgggt ttccccgaga cgatttgcct cattcatttg catgaatgcc cttgtcaaat
     2401 tatttgttga acgtacaata aaaatattac acatacatgt ttgccaaatc aacagaggga
     2461 ttaccagatg gcaataaatg gcggccgggg ggaaacggga aatcatggct gacgcgatcg
     2521 cggcggcgcg cgtgcaagcg ccgtattcaa ggccgggctg gatggcgtgg ctcacgtcgc
     2581 acaaggaatt cctggtgctg tccgttcccc tggtgctggc cctggtggtg ttcttcatcc
     2641 tgccgctgct caatgtgttc ctgctcagcg tcaccgaacc gtccaccggc ctgggcaact
     2701 acgcgctgct ggtgtcgagc gagggcatac gccacatact gtggcgcacc gtgcggctgt
     2761 gcgcggtcac gacggtgttt tccgttgcgg ccgcctatct cgtcgcctat gtgctggcgg
     2821 caagcacgcc gaggcaacgc agcctgatga tgttcttcat cctggtgccg ttctgggtgt
     2881 cggcgctggt gcgcgcgttt gcctggctgc tgctgctggg gcaggacggc ccggtcaatc
     2941 aggcgctggt cggcctgggc gtgatggccc ggcctttcca gttcctcttc aacgaggtcg
     3001 gcgtgaccct aggcatggtg cattacatgc tgccttacgc cgtgctgcca ctctacgccc
     3061 tgatgaaaga catcgatccc cagctgatcc gcgccagccg gggcttgggc gcgacggcct
     3121 tcggcgcctt catccatacc tacctgcccc tgacggtgcc ggccatcgtc gcgtccagca
     3181 gcctggtgct ggtctacagc ctgggcttct acatcatccc cgccatcctg ggcggcgggc
     3241 gcgtgctgat gatcgcccag tacatcagca tcaacgtgct ggaaaccgtg cgctggggcc
     3301 tggcgagcat gctgtcggtc acgctgctgg tcagcgtgct gctgttgtcg cttgccacgg
     3361 ggcgcctggg caggcgcgcg acgcagggag ggaacgcgtg atggatcaag ctttgtccgg
     3421 gtggcgcttc cgcggcctgc aggcgggggc ctgggtggtg ctggcgtttc tctttttgcc
     3481 cttgctcgtc gtcattccgg tgtccttgtc gaaccagtcc tatctctcgc tgccccggca
     3541 cggcttgtcg ctgcagcact acgacaaggt gttcggcgat ccggcatggc gcgccagcat
     3601 cggcctgacg gcggcgctgt cggcggtctc ggccttgatc gccaccgtgc tcgcaggcat
     3661 ggccgcgatc gcgtgctggc gcttccgcgg ccctggcgcc tgggcgctgc gggcgctggt
     3721 gatggcgccg ctggtcgtgc cttccatcgt cagcgcgctg ggcttctatc agatgtgggc
     3781 caagctgggc ctgctcgata cctatgcggg ggtggtcatc gccaacgtcg tgcagaccat
     3841 cccgtatgcg ttcacggccg tgtcggcctc gctattgctg ctggacccgc agttggaaaa
     3901 ggcggcgcgc aacctgggcg ccacccagtt cggcgcgatc tggacggtcg tcgtgccggc
     3961 gacgcgcgcg gggattttct ccgggatgct gttcgccttc gtctatgcct gggacgaggt
     4021 ggtggtgcag ctcttcatca ccagccgggg ggtgcggctg ctgccgcggc tgatctggga
     4081 aggcctgcag gacacggtcg acccggccat tgcggtgatt gcctcctgcc tgaccgtgct
     4141 gacggccgtg ctgattctgg ccatgggctg ggcgcagaac cggaaaaaat aaaagcccaa
     4201 aaaaattgtt ggacatacag caaattaaaa gatagatttt acagatcggt atcgggccgg
     4261 tcgtttgttc gaaaaaccaa ggggaatgca ccatgactat ctcgaaaaac gggggtgtga
     4321 accgccgcga tgtactcaag ctcggcacgg ccctcgcggt cagcccgctg ctggccggca
     4381 cggcccgggc tcaatccgcg cccattacgc tggtgaactg gggcggcgat tccctcaagg
     4441 gaatgaccga tgccttcgcg gcgccctttg aaaaggccac cggcatcaaa gtgcaggtcg
     4501 atacgtccgg ccccagcgcc ggcaagatcc gcgccatggt tcagagcggc aaggtgacct
     4561 gggatgtctg cgacggcacg gtcggctatt gcctggaact cggcccggcc ggcctgctcg
     4621 aggaaatcga ctatgccatc gtgcccaaga ccgcgacgct cgactcgttc gcgttcaagt
     4681 ggggcgtggg cagctatgcc tacagctccg tgctggcctt cgacaaggaa aagttcaagg
     4741 acaaggcccc gtcgaactgg gccgacttct ggaacctgaa ggactttccc ggccgccgcc
     4801 tgctgcgcgc ggacctgccc accatgctcg aggcggcgtt gatggccgat ggcgtcgatc
     4861 cggccaagct gtatccgctg gacaccaagc gcggtttcga gaagctgcgc gagatcaagg
     4921 aaaacaccat tttttggaag accgccgccg aatcggcgca gctgttgcgc aacggcgagg
     4981 tcgtcatggg caatatctgg agcacgcgcg ccgcgccgct gtccatggag cccaacagca
     5041 agatcggctt cacctggaac cagggcatcc tgcaatccgc cgtctggatc gtcccgaagg
     5101 gcaacccggc gggccgcgaa gccgtgatga agctgatcgc gttcatgcag cagcccgagc
     5161 cggcgatcgc cctgagccgc gtgctgtatt gcggcccgat gagcccggcc gccgcggctg
     5221 ccctgccggc ggacctgcgt ccgctggtgc cgaccgcgcc ggagaacgtc aaggtccagg
     5281 tgcccatgga cggcatgtgg tacgcggaaa actaccaaaa agagctgccc aagtatctgg
     5341 acctgatcgc gtcctgaccg ggcgcggcgc aggggccgcg gcaaaaagcc aagaagcgtt
     5401 ttatcaagga gttttcccat gatcgatgtc gacgaattcc gccgcctgtt ccaggcgcag
     5461 gtttcctcgg ttgcggtgat cactgcccag gccggcgaac gccgcgccgg ctttaccgcc
     5521 accgcggtct gttcgctgca ggacgatccg ccttcgctga tcgtctcgat caaccgcaag
     5581 accaacgcct atccgctgat cgtcgagtcg aagcggttcg cggtgaattt cctggctgcc
     5641 gaccaggagc acgtgtcggc gcgattcgcc aagcacgcgg ccgacctgga cgaggcggcc
     5701 atgaagttcg acgccgcggg cgattggtgc ctgtcggccc atggcgcgct gctcctgcag
     5761 aacgccacgg ccgtggccga gtgcgagctg gtccagtcag tcgagctcaa cacccacgcg
     5821 atcctgatcg gcaacatcct ggacgcggag gtgtcggaga cggccaagcc gctgctctac
     5881 gggttcaagc gctacgtccg catccctgaa acccgggagc aacacgcctg atcatgaaag
     5941 acagcatgct tttgtccatc gacggcttgc gcaaggccta tgccggcaag gcggtctcgc
     6001 cggtcgaggt gatgcggcac acgatcggcc agatcaacgc gctcaacccc gagctcaatt
     6061 ttgtctacga gatgcacgag gaagaggcgc tgcgcatggc ggccgagtcc gagcgccgct
     6121 ggatggccgg ccggccggcg ggattgctgg acggtattcc cgccaccatc aaggacgcct
     6181 tgccgctggc gggctactgc agttatcgcg gcacggcggc cgaagatccc gatcccgtgc
     6241 ccagcacgac ggacgcgccg tgcgtggcgc gcctgaagga agagggcgcc atcgctttcg
     6301 gcaagacgac tatgtgcgac ctgggcatcc tgcccagcgg cacgggctcg tcctacggcg
     6361 tgacgcgcaa tccctggaac cccgcgcgca acccgggcgg atcgagcgcg ggagccgccg
     6421 cgagcgtcgc cgccgccgtg catcccttcg cgatcggcac cgacattgtc ggctccatcc
     6481 gcctgccggc ggcgtggagc ggcatctatg gcttcaagcc ttcgcagggg cgtgtgccgt
     6541 attactttcc caacagcccg tcgctggtcg ccgggccgat gacgcattcg gtgcgcgacg
     6601 cggcggaact gatgaacatc atcgcccggc ccgatccgcg cgactttgcg gcgctggcct
     6661 acgacgggtt cgactatctg gcgcagctcg atgcgccgat caagccaggc cgcatcggta
     6721 tcgtccgcga cctgggcttc gacgccaagc cggacacgga aagcctgggc gtgctcgacg
     6781 ccgcggcggc cattctcaag agcatgggct gggaaatccg cgacatcgcg ccgccctgca
     6841 cgcagcagga cctgctggcc gccgagaatt tctaccgcgt gcgcgcctac gccgagctca
     6901 agaccaagcc catggcgcgg cgggagaagg ccgcggtggt caacggctgg gcgagcgcgg
     6961 cggcctcggc ctcggcgagc gacttctacg actggttcaa caccctgcag cgctgccgcg
     7021 agcaggtcgt gtcgatgatg cgctacgtcg attacctgct gctgccgccc accctgacgg
     7081 ccgcgccgga agccgaattc ccgcgcagcg ctgccgatgc gctgttcgtg ccctgggcgc
     7141 agacatacct cttcaacctg tccgagcagc ccgcggcgtc ggtgcccatg cgcctgtcgg
     7201 gcgcgggcct gccgctgggc gtgcagatcg tcggccggcg tttcgacgac gccggcgtgt
     7261 tgcgcttgtc ggcgcgcctg gaggcggcgc gcggcaggtt ccccctcagc ccctatgcgc
     7321 aacgcggcat tcaaggcttc aacgaaagac tcaacgaaat cgctcgaggt taattatgag
     7381 attcacgcta ctggtcattc cttatctgcg cgagcgcggg gaaaaagtcg gcgcggtcgg
     7441 cggcgagccg gcgcgcttcc agcgcctgat gcagcagctc aagacgcaga tggtcttcgc
     7501 cgaatcggtc ggctacgacg gcttctgcat gacggagcac catctgcagg tcgagggcgt
     7561 cgaatccacg accaaccccc tgttctggga ctacttcgtg gcgcagcaca cgaagaagat
     7621 gcgcgtcggc cagctgggca tgaacctgac ggtgatcaac cccatccagc tggccgagaa
     7681 catcgccatg ctcgaccact tgacgggcgg gcgcgtgttc gcgggcttct cgcgcggcaa
     7741 taccgcgcgc tgggccaaca ccttcggcca gcatatcggc atcggctcga ccgaatccga
     7801 ccgttcggcg gccgaccagc gcaaccgcga gattttctac gaaaactgga acatcgtgaa
     7861 gcagttgtgg acgcagccta ccgtcgacat cgacggcaag tactggcagg tgccgccgga
     7921 gatgcactgg cacttcgcgc cgaccgacga ctgggcgccg gaaacgctgg gccccgacca
     7981 cgcgctcaag cgcgtgggca tcgtgccccg cccgctgcag acgccgcatc cgccggtgta
     8041 cgcgccgttc agctacagca tggagacgtc caagttctgg gctcgcgaag gaggcaagat
     8101 ggtgtccttc gtggcgccgg aaaaggagag cttcatccgg accgcgctgg acgtctacat
     8161 ggccgaggcg gagcaggccg gccgccagac cacgacgcag gattcgctgg cgatcggcgg
     8221 ccacctggtg tgcgggtcca ccccggccga aagccgcatc atcgaggacg gctttcgcga
     8281 acttttccgc tatgcctacg atgcgccgcc gtatcatgtt cccatgggcc ggctgtggaa
     8341 gggctcgcgc cagcagatgc tcgacgacgt cgcgacgctg gcggaaaaat tcggtgtcga
     8401 ggaattcttc tgctggcacc acgtgggctg gttcacgcaa gagcaggaga tgtcggcgct
     8461 caacgaattc gccgaagcgg tcatcaagcc gctgaacggg gaggtcaaac tgtgattggg
     8521 ggcgagcatg ctcacgctgg gtgatttgtg tcggcgcaac gccgaacgct ggaccaacaa
     8581 ggatgcctat gtcgaggaag aacggcgcgt aagctggggc gagttcgacc gccggaccga
     8641 ccagctgggg gcggccctgc gcgccatggg cgtcaagccc ggcgaccggg tggcgatgct
     8701 gggctacgac tgcgtcgaga cggcggaggt cttcatggcc tgcgccaaga tcggcgccgt
     8761 gcgcgtgggc ctgaacccgc gcctggcggc gcgcgagatc gccgcgctga tgcaggactg
     8821 cggcgcctcc atcctgttcg tcgcggctag cagcgaggcc ctggtgggcg aggcggcggc
     8881 ggcccagccg gcgctgcccc tgatcggctt cggcgcgggg catgcgcagg ccatcgacta
     8941 cgaacgcctg atcgccacag agcacggcat cggccggctg ccgcagacgc cgtcgccagt
     9001 cgtcatgatc gcctacacca ccggttcgac gggcctgccc aagggcgcgg tctacgagca
     9061 tgggccgttc ctgcaatcca tcatgatgat cgccctgtgc gagggcgttg acagcagttc
     9121 ggtctggctg cacgccatgc cggcggccgg cattccgatc atgcacatgt tccgcaacat
     9181 cttccacggc tcgacgacgg tgatcgtcgg tccctgggat gcgcagaagg ccttgcggct
     9241 gatccggaac gagcgcacga cgaacacggt gctggtgccg acgatgctca attcgctgct
     9301 ggcgtgcggc gacctgggat ccaccgacac gagttcgatg cggctcctgg gctacggcgc
     9361 ctcgacgctg ccggccgcga cgatccgggc cgcgatgcag gccttcgatt gccccttcct
     9421 gcagatgtac ggcaccaccg agctgatggg catgggcatg atgctctttc ccagcgacca
     9481 tgcgttgggc ctgtcgacgt atcccgagat actgacctcc accggcaagg ccttgcccta
     9541 cgtcgacgtc aaggtgatcg acgacgacgg caatgtgctg ggcgcgggcg aggtcggcga
     9601 actcatcatc tacagcgaat accgcttcaa ggaatatctc aacaacccgc agcagacggc
     9661 ggacgcgctg cgcgacggct ggatccatac cggcgacatc ggccaggttt cgcccgaggg
     9721 ctatgtgttc ctgcacgacc gcgccaagtt caagatgaag acgggcggct atctggtcta
     9781 tccgaccgaa gtcgaaaatg ccctggccga gcacgacgcg gtggacgagg tggcggtggt
     9841 cggcatcccc gacgagcgct ggggcgaccg catcgaggcc ctgatcacgc tcaagcccag
     9901 cacgcgcgtc gacgaggccg cgctgctggc gttctgcaag gaacgcatcg cttccttcaa
     9961 ggtgcccaag cgcatcgtca tttccgacct gatgcccaag gggccgacgg gcaagatcca
    10021 gaagctcgat gtcatcaagg tgctgaagca atcggccgag caggctggcg gagcgaaggc
    10081 gtgagcggcc cggcgcccga ggccggcccg cagcagcgcg tcgtcgacgg cctgcgccag
    10141 ggccggctgc tgctgcagaa atgccgcagc acaggggaat acctgttcta tcctcgcgcg
    10201 gcggcccgcg gcgtgcgcag cggcgattgg gacctggtgc cggcctgcgg cctgggccgg
    10261 gtctatgccg ccacccatgt cggcatgcgc ggcgcgccga gctatcacat cgcgctggtc
    10321 gacttggcgg aaggccccag gctgatggcc agactggaat ggcctggcga tatcccgcct
    10381 ccgcccgaca cgccggtgcg catgaaaatg gtcgagcccg agtgggcgcc gcaactgggc
    10441 catcccgtgc cggtcttcga gccggcgggc gccgggggcg gcacaccatg acccaccctt
    10501 tgcgcggcgc ggccgccatc gtcggacagg gctattcgca gctgggcagc gtgcccggcg
    10561 tcagcgccat ggagttgctg gcgcaggccg ccgagcgggc cgtggaggat gccggcctgg
    10621 acttcagcga tatagacggg ctgtgcgccg gcaccttcta ccatttctgg cccacgcttt
    10681 ccgtcgcgga gtacctgcgc atcgagccgg cctggtcgaa tgccgacatg gtgggcggct
    10741 cgtcgttcat ggcgcacgtc cagcaggcgg cgctggccat ccaggcgggc ttgtgccata
    10801 cggtgctgat cgcctatggc agcaacgctc gcagcctgcg cgacggcgct ggcctgatcg
    10861 agacgccctg gaccgagcgc tggtcggcgc cggccatacc gatcgccggc tatgcgctgg
    10921 cggcgacccg gcacatgcac cagtacggca cgacacgcgc tcacttggcc gaggtggcgg
    10981 tggcggctcg caagtgggcg caacgcaacc ccctggcgac cgcgcgcgac gagctggcgc
    11041 tcgatgacgt gctggccgcg ccgcagatcg ccacgccatt ttccaaatac gactgctgcc
    11101 tggtttccga tggcgcggcg gcgatcgtgc tgacttccgc tgaccacgcg gcgcacggca
    11161 ggcgcaagcc ggtctacgtg ctgggcgccg catcggcgca ccggcaccgc gagatcgcgc
    11221 aaatgggcga cctcacgagc accgccgcgg tccagagcgg cagggccgct ttcgcgatgg
    11281 ccggcgttgc gcccaaggac gtggatgtcg tccaggtcta cgacgccttc accctcaaca
    11341 cgctcctttt cctggaagac ctgggctttt gcgccaaggg cgagggaggc gccttcgtcg
    11401 aaggcggcgc catcgcgccg ggcggccggc tcgcggtcaa cacgagcggc ggcggcctgt
    11461 cgttcgtgca ccccggggct tacggtttgt tcggcctgtc ggagtccgtg ctgcaactgc
    11521 gcggcgagac gggggcgcgg caggtcgagg gcgccaggct ggccgtagcg cacggcaacg
    11581 gcggaacgct gtcgcaccag tccaccgtgg tgctgggcac ggccgattat ttgtaagact
    11641 cattgcgccg ccgcgatgtt gaccatccgc cggcctggat tttaggagaa cgacatgcaa
    11701 ggccaagaac aactgcttgc tttcgcgcag gaatatttcg aagtgctcca tacctgcgac
    11761 gtggtgcgcg ccgccaagat ctttcatccg gaatgcggcc tgttcaatgt ggtcgaaggc
    11821 aagatcaatt tcgtgccggg ccgccagtac ctggagatcc tcaagggtcg cgagtcgccc
    11881 aggagccggg gcgagcccgt ctacggcaag gtgctgacga tcgaccagac cgacgagaac
    11941 accgccttcc tgaaagtgct gtcttccgtg cagccgcgct acttcgaaga ctacctgacc
    12001 ctgctgcgcg aagatggcca gtggcgcgtc gtcgccaagg tctaccgcgc cctggccgcg
    12061 tgccgcgacg actgactcaa ggctttccaa ggacatcgag ggcggcaagt cctggccggt
    12121 ttcgcaagac gcttgagcct ctgtcccgaa cgcgacaaaa accaacgagg aaacaagcga
    12181 tgagccatgg caggatgtcg atcgggctgc attttcatcc ggtgggaacc cacccggcgt
    12241 cgtggctttg ctcggatgcg cccgcgggcg cggaagtcag catcgactat tacgccgagg
    12301 tggcgcgcat cgccgagtcg ggcgcgctcg acttcatgtt cttcgccgac gtgccggcca
    12361 tgcgcgacgg caatatggcg gccagccgcc gctggccgct ctacgcggcg cagttcgagc
    12421 cggtcacctt gctgggcgca ttggccgcgt cgacccgccg gctcggcctg gccgcgaccg
    12481 tctccacctc gttctacgag ccgtacaacc tggcccgcca gttcgcctcg ctcgatcacc
    12541 tgagcaaggg gagggtggcc tggaacgtcg tcaccacgtc gtcaggcgcg gcggcgttca
    12601 acttcggcag aaacccgcgc caggaacacg cgcagcgcta tgaacgagcc agggagttcc
    12661 tcgccatcgt caaggggctg tgggacagct gggacgacga cgcttttccg cgggacaagg
    12721 cgtccggggt tttcttcgac cccggcaagc tgcaccgcct ggacttcaac ggcaagcact
    12781 ttgcggtgcg cggcccgctg aacgtgccgc gttcgccgca gggccatccc gtcatcatcc
    12841 aggccggcgg ctcggaaacc ggcaaggctt tcgccgccga gaccgcggag gtggccttca
    12901 ccacggaaag cagcctcgct ggcgcacaga agttctatgc ggacctcaag ggacgcctgc
    12961 ccggcttcat gcgcacggaa gatcaattga agatcctttc cggcttgaag cccattctgg
    13021 gcatgaccaa gacggaagcg gaagacaagt tcgacaggct gcaacgccat atccaccccg
    13081 acgtgggccg cgagatcctg tcgatcgacc tggacggcgt ggacttgtcc gatgtgccgc
    13141 tcgacagccc catccccgac gacaagatcc ccaaggatgt cgaaggcggc aagtcctact
    13201 tcggctacct ccgcaagttg atggacaacg ggcccatcac ggtgcgccag ctgtacgaaa
    13261 tctatgcgcc ggcgcgcggc ggcaacttca tcgtgggcac gccgaaggac atcgccgaca
    13321 tgatggagga gtggttcgtc ggcaaggccg cggacggctt catgatcggc ctggcctatg
    13381 tgccgtgcga tgcgcgtctg ttcgtcgagc acgtcgtgcc ggcgctgcgc aggcgcgggc
    13441 tgttccggct ggcctacgaa ggcgcgacgc tgcgcgatca cctcggcttg gcgcggccgg
    13501 tgtcgcgcta cgcatcgcgc tagcgggtct cgcgtgttgc gctcagggaa aacgatagcg
    13561 ccctgaaagt cattttttgt tgaatgttca acatatattg agatagcctc tttaaacgac
    13621 gcgacgcacg cacgtcgctc atacccggag taaagatgag cgctcgattc atgcacatcg
    13681 gtgcattgct ttcccatgtg ggaatacatc ccgcggcctg gctggaacct ggcgtgccgc
    13741 ttggcggcga ggtcgatctg gccaactgga tcggcatggc caagacggcc gaacaggcca
    13801 agctcgattt catcttccgg gcggattccc ccgcgagccg cgaaggcgac ctcgaggcca
    13861 tcacgcgctt tccgggccat atcgcggaac tggagccgct cacgatcatg tcggcgctgg
    13921 ccgcggtcac caagaacatc ggcctggcca gcaccatttc caccagcttc tacgagccct
    13981 acaacgtggc gcgcctctac gcctcgctgg atcacatcag cggcggccgc gccgcgtgga
    14041 acgtcgtgac gacctcggcg tcgatcgccg ccaagaactt cggcaaaacc ggactcgaag
    14101 accatgccca gcgctacgcg cgcgccaacg aattcgtcga cgtggtgacg gggctctggg
    14161 acagctggga cgaagacgcc tttatccgga accgcgcctc gggccgcttt ttcgatccga
    14221 gcaagcgcca cgtcctcgac tacaagggcg agttctacag tgtccgcggc ccgctggacg
    14281 tgccgcgctc gccgcagggg cgcccggtga tcgtcaatgc cggcggatcc gaagcgggca
    14341 tggaactggc ggcccgcacc gccgaagtcg tgttcagcgt ggaccgcagc ctggaaaagg
    14401 ccaaggcctt ctatgccaac ctcaagggcc gcatggccaa gtacggcagg cgcccggaag
    14461 cgttgaagat cgtgtgcgcc ttgaatccct acgtcggcga gaccgacgcg caagggcagg
    14521 aaaagctcga ctatctgcag tcgatgatcc acccggcggt gggccgcgag atcatctcgg
    14581 tggatctggg cggcatcgac ctgcacaacc tgcccctgga cgaacccatc cccgagtccg
    14641 ccctgcccac ggcgagcaac gcgaccaagt cctatttcga caatatggtc gccgcgatca
    14701 gggaagacaa gctgacggta cgccagctct acctcgcgtg cgcggcctcg cgcggcggca
    14761 tgaatgcgat tgccggttcg ggcgccacca tcgccgaccg gatggagcaa tggttcaagg
    14821 agggggctgc cgacggcttc atggtgcgcg tctcgcacct gccgggcggg ctggaggatt
    14881 tcaccaagta tgtgatcccc gagttgcagg agcgcaagct ctttcgcacc gagtactcgg
    14941 gtcccacgct gcgcgaccaa ctgggcctgg cacgtccgca atcgcgttat cgaccaacca
    15001 gcagttgatt cggagaatcc catgaaaaag caggtccagc agcatctggg tgtgccggcg
    15061 ggcacggtac aggccaagcg gcgcagtgtc ttgaaggccg cggcggcggc gggcattttc
    15121 ctgccggcga tggcgggcta ccagcgccgc gcgttcgcgc agaccaaggt ggtcaacttc
    15181 gcttcctacg gcggcagcta cggcgacgcc ttgcgcaagg cgtggctgga tccgttcgag
    15241 aaagaaaccg gcatcaaggt caacctcggc gtgaacgcca atctggcgct ggccaagctg
    15301 cagacgatga acgccaacgg tgccgagtgg gacatcgtcg acctgtcggg tcaggaatac
    15361 agcatcgccg ccaaggaaaa catcctgctg ccgctcgacc acaagctgat cgatacggca
    15421 aagctggaac ccgatttcgt gaagagccac ggcttcggct atgcgtccta catctggggc
    15481 atggcttggg accggcgcaa gatcccggac gagaccgcgc ccaagagctg gcccgagttc
    15541 tgggacaagc aaaaataccc gggcaagcgt tcgctcatcc aggtcaatac caacggccac
    15601 gcgatcgagg ccgccctgct ggctgacggc gtgccggttt ccaagctatt tcccctggat
    15661 gtgccgcgct cgctcaagag cttggacaaa ctgggccgcg acaacatcgt gtggaacacc
    15721 agcaaccagg agcccgtgca gcgcctgacc tcgggcgagt gcccgctggc cgggatgttc
    15781 gtcgggcgcg cgctcatggc caaccgctcc ggcgccagca tcggcttttc gttgaacggc
    15841 gccaacatcg ccccggacta cctgggcgtg atccggaccg ctcgcaacaa gccggaggcc
    15901 cacgccctgc tcaactacat cgcgacccgc gcggaccgcg cggcggagtt cacgacgatc
    15961 acttcatatc cggtgcccat tcaggggctg gcggccttga tcccggcgag cgccgccgat
    16021 atcctggctg tcctgccgag cgatccgaaa gtgaaggctc agatttacga agacaacggc
    16081 gcgtattggg cggaaaacct gccgcgagtc gcggcgatgt tcaagcagtg gcagttgacc
    16141 tgatttcagg gttggcatga tgggcggacc gcctggagcg atcgaggcgg tccgcccttg
    16201 tcatgatggc ttgattttcc ggggtatgaa aaatgcaacg taaatacaac ttggcgcagt
    16261 ctttcctgct gacgcccgtc gtgctgttgt acgggctggc tgccgtggcg ccattgctgg
    16321 tgatcatcga gttgagcctg caaaaaggcc ttgcgactta cctggaggtg atcgcttcgc
    16381 cgctcttttt caagacggcg atcaatacgc tggccatttc gatcgagacg acggtggcgg
    16441 cggttgtgct tggctatctg gtgtctgctt tgctggtgca ggccaagggg ggctggcggg
    16501 tcctgatcat ggcgatggtg ctcctgccgt tctggacctc ggcgctgatc aagaacttcg
    16561 cctgggccgc cttgctccag gacaacggcg tcatcaacgg cctgctcatg cggctgggtc
    16621 tggtcacgca gcccctgacc ctgctgcata cccgcttcgc ggtggtcgtg gggatggtgc
    16681 actacgtgat cccctacgcg gtgtttccga tctacagcgc gatgctcgcc atcgatccgg
    16741 cgctgtaccg cgctgcgcag agcatggggg cttcccgtct ttcaagcagc acccacatca
    16801 ccctgccgtt gacgcggccc ggactgtacg cggcgttcct gctggtgttc atcatttcga
    16861 tcgggttcta cataacgccc atcgtgctgg gcagcccccg cgacatgatg gtggccaacc
    16921 tggtggattt ctacacgcat cagctcatcg atttttcctc ggcctcggcg ctggccatcg
    16981 tcattctcgt ggtgacggcg ttcttcatcg ccctgtacaa ctcgatcccg aaggaggggc
    17041 agtatggcaa gatctaatac cttgctgctg atcgcctgcc tgccgatctt cgtcttcctg
    17101 ctcggcccga tcctgatcat tgtgcccatg tcgctgacgc agggagatat cctggtgttt
    17161 ccgccctcgg gtgtctccct gcattccttc tttgatctgt tcatggaccc gcaatgggtc
    17221 tcttcgggcg tcacctcgtt caaggtcgct ttgctggcga cgcttatctc gaccctcgtc
    17281 ggcaccgcgt cggcgctcgc gctgcatcgc aatgccgtgc ccggccgcgg cgccttgtcc
    17341 acgctgatca tgctgccgct cttgatgccg gtgatcgtgc tggccctggg cgattatctg
    17401 ttcagttcgc ggatcgactt gagttcgaag tgggtggcca tcgccatcgc gcatagcgtg
    17461 gtggttaccc cttatgtttt cgtttcggtg cagacctcgc tcgcgggact ggacccggcc
    17521 ctgcatcggg cggcgcgcag ccttggcggc gactcctggg cgctgctggc gcacgtgtac
    17581 tggcctgcga ttcgttccgg ggtgctgggc ggggcattgt tctcgttcat cggatcgttc
    17641 gacgaggtgg tgatctcgct gttcctctcg gggccggatg ccgtcaccct gcctgtccag
    17701 atgttcacgg ccatccaata cgacctcaca cccaagatcg ccgcggtatc gaccgtcttg
    17761 ctggcgatca gcgt
//
LOCUS       DASWPO010000228         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_139520, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000228 DASWPO010000000
VERSION     DASWPO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139520"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VGC69_12115"
     CDS             complement(<1..382)
                     /locus_tag="VGC69_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296067.1"
                     /translation="MLEQQIVNALSLGCVYALFALGFTLVFGVLGIINLGHGAVFMVG
                     AYAALMVIIHLGLPLWAAIIASMVAAGLVGAVIDVLVLKPLRKRNAPQLIPMIATIGT
                     AIILTNGIQAVFGADNQRFPAGILP"
     gene            complement(476..1624)
                     /locus_tag="VGC69_12120"
     CDS             complement(476..1624)
                     /locus_tag="VGC69_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296068.1"
                     /translation="MKSVLKLAPVVAALLAGSVHAADIKIGAVESLSGGAAQYGNNIR
                     NGLQLAADEINAAGGVNGNKLVLAVEDDQTKKEEAINAFKKLIFQDKALMVFGPTLSN
                     TALAAQPIAQAARTVAFGTSTTVDGITDPGEYIFRNAVTESAVIPVTVKTAQAKLGLK
                     KVAVMYGNNDALSKGGYDIFKKALEDNHIDVTDTETFSTGDVDFKAQLTKIKGTNPDA
                     IVLSALIAEGAPIVAQARQLGITVPFIGGNGMNSPKLFDLASGTASDNLWVGSPWASD
                     NASPENTKFIADYKARYNAAPDQFAAQAYDAVHIASSAMKKVQFSGDLAKDRAAFRDA
                     LASVQWTGATGAFKFVQIKNKAGKMAGYDAEQTPIISVTKSGKYDIVK"
     gene            1917..>2730
                     /gene="rocF"
                     /locus_tag="VGC69_12125"
     CDS             1917..>2730
                     /gene="rocF"
                     /locus_tag="VGC69_12125"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY0296069.1"
                     /translation="MGPEALRVAGIAEMLVRRGLAVVDRGNLHGPANPWLPPQGGYRH
                     LGEVVAWNRLVHDAVYAALAAGELPVLLGGDHCLGIGSISAVARHCAAAGRKLRVLWL
                     DAHADFNTSVITPTGNLHGMPMACLCGDGPAPLVAMSGRTPAIEASWVRQIGIRSVDD
                     GESQRVRASQLEVYDMRYLDEAGMRMVMESALMGLDDDTHLHVSFDVDFLDPEIAPGV
                     GTTVPGGPTYREAQLCMEMIADTGLVRSLDIVELNPALDVRNRTAQVAVDLVE"
BASE COUNT          421 a          797 c          995 g          517 t
ORIGIN      
        1 ggggcaggat gccggcggga aagcgctggt tgtccgcgcc gaacacggcc tgtatgccgt
       61 tggtgaggat gatggccgtg ccgatggtgg cgatcatggg gatgagttgg ggcgcgttgc
      121 gcttgcgcag cggcttgagg accagcacgt cgatcacggc gccgaccagg ccggcggcga
      181 ccatggacgc gatgatggcc gcccacagcg gcaggccgag gtggatgatc accatgaggg
      241 cggcgtaggc gcccaccatg aacaccgcgc cgtgtcccag gttgatgatg cccagcacgc
      301 cgaagaccag cgtgaacccc agggcgaaca gcgcatacac gcagcccagg gacagggcgt
      361 tgacgatttg ttgttcaagc atgacggatg ccggatgttg aaggataagt acccgatgac
      421 ataaacgcat aagggcgacg cagggtcgcc cttatgcggc ttggcgctgc cgcgcctatt
      481 tgacgatatc gtatttgccg gacttggtca cgctgatgat cggggtctgt tcggcgtcgt
      541 agccggccat cttgcccgcc ttgttcttga tctggacgaa cttgaaggcg ccggtggcgc
      601 ccgtccactg gaccgaggcc agggcgtcgc ggaaggcggc gcggtccttg gcgaggtcgc
      661 cgctgaactg cactttcttc atggcgctgc tggcgatgtg cacggcgtca taggcctgcg
      721 cggcgaactg gtcgggcgcg gcgttgtagc gggccttgta gtcggcgatg aacttggtgt
      781 tttccggcga ggcgttatcg gacgcccagg ggctgccgac ccacagattg tcggaagcgg
      841 tgcccgaggc caggtcgaag agcttgggcg agttcatgcc gttgccgccg atgaagggca
      901 cggtgatgcc cagctggcgg gcctgcgcca cgatgggggc gccttcggcg atcagcgccg
      961 acagcacgat ggcgtcgggg ttggtgccct tgatcttggt gagctgggcc ttgaagtcca
     1021 cgtcgccggt cgagaaggtc tcggtgtcgg tgacatcgat gtggttgtct tccagggctt
     1081 tcttgaagat gtcgtagccg cccttggaca gggcgtcgtt gttgccatac atcacggcga
     1141 ccttcttcag gccgagcttg gcctgggccg tcttgaccgt gaccgggatg accgcggatt
     1201 cggtgacggc gttgcggaag atgtattcgc caggatcggt gatgccgtcg accgtcgtcg
     1261 acgtgccgaa ggccacggtg cgggcggcct gggcgatggg ctgggccgcc agggcggtat
     1321 tggacagcgt ggggccgaag accatcaggg ccttgtcctg gaagatcagt ttcttgaagg
     1381 cgttgatggc ttcttctttc ttggtctggt cgtcttcgac ggccagcacc agcttgttgc
     1441 cgttgacgcc gcccgcggcg ttgatttcgt cggcggccaa ctgtagaccg ttgcggatgt
     1501 tgttgccata ctgggcagcg cctcccgaca gggattcgac ggcgccgatc ttgatgtctg
     1561 ctgcatgaac ggagcccgcg agcagcgcgg caaccaccgg cgcgagttta agcacggatt
     1621 tcatgggggt ggatccttgg aatgggttgt ctcaagatgt ataccggcca ggggtacggc
     1681 cggtggcaac ttcgcggcgg ttgaccgggc tgccagggtt tgtgatagta caccaattgt
     1741 tgcgaatcaa taccttaggc gttcttgaaa ggagtttgca agattttgtt ccatcaccat
     1801 ccgcgagccg ggcacccgtg acccaaggcc tttccgtgac cgtttatccc ccggggttga
     1861 tcgccgcctt gatcggcgcg cccaccgaca tcggcgccgg tgtccgtggc tcgtcgatgg
     1921 ggccggaggc cctgcgggtg gccggcattg ccgagatgct ggtgcggcgc ggcctggcgg
     1981 tggtcgaccg gggcaacctg cacggcccgg ccaacccctg gctgccgcct cagggcggct
     2041 accggcactt gggcgaggtg gtggcctgga accgcctggt gcatgacgcg gtgtacgcgg
     2101 cgctcgccgc cggggagctg ccggtgctgc tggggggaga ccactgcctg ggcataggct
     2161 cgatcagcgc cgtggcccgg cactgcgccg cggccggccg caagctgcgc gtgctgtggc
     2221 tcgatgccca cgccgatttc aataccagcg tgatcacgcc gaccggcaat ctgcacggca
     2281 tgcccatggc ctgcctgtgc ggcgacgggc ccgcgccgct ggtcgccatg agcggccgca
     2341 cgccggcgat cgaggcgtcc tgggtacggc agatcggcat acgcagcgtc gacgatggcg
     2401 agagccagcg ggtgcgcgct tcgcagctgg aggtctacga catgcgctat ctggacgagg
     2461 cgggcatgcg catggtgatg gaatcggccc tgatggggct ggacgacgat acccatttgc
     2521 acgtgagctt cgatgtggat ttcctcgacc cggagatcgc gccgggcgtg gggacgacgg
     2581 tgccgggcgg gccgacctac cgcgaggccc agctgtgcat ggagatgatc gccgacaccg
     2641 ggctggtgcg ttcgctggac atcgtcgagc tcaatccggc cctggacgtg cgcaaccgca
     2701 cggcccaggt ggcggtggac ctggtggaga
//
LOCUS       DASWPO010000229         6655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2463473, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000229 DASWPO010000000
VERSION     DASWPO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463473"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGC69_12130"
     CDS             <1..346
                     /locus_tag="VGC69_12130"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0296070.1"
                     /translation="VKGVSIGDGFASIAQRGSEHGDEITPEGFASNHAGGVLGGISTG
                     QDITVSLAIKPTSSIRIERRSINRAGEPVMVQTLGRHDPCVGIRATPIAEALLALVLI
                     DHALRQRGQRGA"
     gene            complement(417..1016)
                     /locus_tag="VGC69_12135"
     CDS             complement(417..1016)
                     /locus_tag="VGC69_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0296071.1"
                     /translation="MARPREFDEAVVLDAAVHCFWSRGYESTSVKDLVNGTGITAASL
                     YNAYGDKLGLFRTALDRYVEHSIAARLRRCAALPPREAIDAFFDDILSRSLNDRQHKG
                     CMLVNSALEMAPHDPEFQQTIAGVLKRLERFFFDCVEAGQEAGTISRALAAEDLARHL
                     LGTLMGVRVLARARPERPLLEGVVAMALALLDQPGGKAA"
     gene            1121..1825
                     /locus_tag="VGC69_12140"
     CDS             1121..1825
                     /locus_tag="VGC69_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY0296072.1"
                     /translation="MIRFYFHPTPNPAKVALLLEETGLPYEVVPVDTSKGEQHEPGFR
                     AVNPNGKVPAIIDTEGPGGKEARVFDSTAILLYLAEKTGKFLGAGEDRPELLSWLLFL
                     ASGLGPFSGQAVHFQYAAPAGLDYAVNRYRREAERHYQVLDDHLEGRTYIVGETYTIA
                     DISAWGWLDRAARVRKGEDDPLGPFPNLKRLFETVDARPAVARARAVGQDHAFKKIND
                     EETKRALFPSNYPPAA"
     gene            1853..2725
                     /gene="yghU"
                     /locus_tag="VGC69_12145"
     CDS             1853..2725
                     /gene="yghU"
                     /locus_tag="VGC69_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEY0296073.1"
                     /translation="MADSPDYTPPKVWTWTQPNGGAFASTNRPVAGPTHDKELPVGRH
                     PLQLYSLGTPNGIKVTILLEELLALGHAGAEYDAWLIKIGDGDQFGSGFVAVNPNSKI
                     PALLDRSGPKPIRVFESGSILLYLAEKFGAFLPKDVAARTETLNWLFWQMGSAPYLGG
                     GFGHFYAYAPTKIEYAIDRFAMEVKRQLDVLDRRLAESAYLAGDDYTIADIAVFPWYG
                     GLVKGWQYGAAEFLSVQDYKHVQRWADAILARPAVKRGRMVNRVRGEPSEQLHERHDA
                     SDFDNKTQDKLAGA"
     gene            2728..3534
                     /locus_tag="VGC69_12150"
     CDS             2728..3534
                     /locus_tag="VGC69_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296074.1"
                     /translation="MKIENKRILITGGSSGIGFALARALLAKGARVAITGRRPDALAK
                     ALRELREISPSVSIITADVTTPDGRGATIGQAIAALGGLDILMNNAGGVRAGRLENTP
                     EAELQAMIDVDLVAPVLLTRAALPALRASGDAMVVNISSGIALIGAPFYAAYAATKAG
                     LARFGESLRREWKGEGIHVLTAYPGGTDTPMMQSNRAGPELGFSREPASTVADAIVEG
                     IEANAFEVVRGGEARAQMIALNRDNPAAVDERFLGLKPALEAAVKDHSAL"
     gene            3675..5084
                     /gene="leuC"
                     /locus_tag="VGC69_12155"
     CDS             3675..5084
                     /gene="leuC"
                     /locus_tag="VGC69_12155"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0296075.1"
                     /translation="MAQTLYDKLWQSHVVHQESDGTCLLYIDRHLLHEVTSPQAFEGL
                     TLAGRKPWRIGANLAVADHNVPTVTAHRAQGIDDPVSKLQVDTLDDNCGKFGITEFKM
                     NDLRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGALATLAFGIGTSEVEHVLATQTL
                     LMKKNKSMLIKVEGELPFGCTAKDVVLHIIGIIGTAGGTGHAIEFAGSAIRALSIEGR
                     MTVCNMAIEAGARSGMVAVDDKTVDYFKGRPFAPTGVLWDQAVSYWRTLHSDPGATFD
                     RVIEVDARQIKPQVTWGTSPEMVLPVDGRVPDPDREKDDVRRSGMERALQYMGLTPNT
                     PLTDIRVDRVFIGSCTNSRIEDLRAAASIARGRRVAANVKEAIIVPGSGLIKQQAESE
                     GLDKIFIAAGFQWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSP
                     AMAAAAAVAGHFVDVRTLR"
     gene            5105..5758
                     /gene="leuD"
                     /locus_tag="VGC69_12160"
     CDS             5105..5758
                     /gene="leuD"
                     /locus_tag="VGC69_12160"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0296076.1"
                     /translation="MQAFTSHEGLVAPLDRENVDTDLIIPKQFLKSIKRTGFGPNLFD
                     ELRYLDHGEPGMDNSRRPLNPDFVLNQQRYQGASILLARKNFGCGSSREHAPWALTQY
                     GFRAIIAPSYADIFFNNSFKNGLLPIILSEFEVAKLFDEVKAFPGYKLIVDLPGQVVR
                     TPGGQAMHFDIEPFRKHCLVNGLDDIGLTLQHADKIRVFEAERLARHPWLEGQPVLG"
     gene            5948..>6655
                     /gene="leuB"
                     /locus_tag="VGC69_12165"
     CDS             5948..>6655
                     /gene="leuB"
                     /locus_tag="VGC69_12165"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY0296077.1"
                     /translation="MTTHQIAVLPGDGIGPEITEQAVRVLTALNLSLDLKQAPVGGAA
                     FDQFEHPLPPATLELAKQSRATLFGAVGDWKYDALPREFRPEQAILGLRKNLGLFANL
                     RPAILYPELASASSLKPEIVAGLDILIIRELTGDIYFGTPRGVRTAPDGAFKGEREGY
                     DTMRYTDPEVRRIARVGFETARKRGKKLCSVDKANVLETSQFWRDIVIEVAREYPDVE
                     LSHMYVDNAAMQLVRNPR"
BASE COUNT         1211 a         2367 c         2062 g         1015 t
ORIGIN      
        1 cgtcaagggc gtgtccatcg gcgatggctt cgccagcatc gcccagcgcg gctccgagca
       61 cggcgacgag atcacgcccg agggctttgc cagcaaccac gccggcggcg tgctgggcgg
      121 catttccacc gggcaggaca tcaccgtttc gctggccatc aagcccactt ccagcatccg
      181 catcgagcgc cgctccatca atcgggccgg cgagcccgtg atggtccaga ccctgggccg
      241 ccacgacccc tgcgtgggca tccgcgccac gccgatcgcc gaggcgctgc tggcgctggt
      301 cctgatcgac cacgcgctgc ggcagcgtgg gcagcgcggg gcgtaaatcc gccggcctgc
      361 gcggcgcggg cggaacacct cccgcttgac gcagcgcggg gcaaggccat cgcccgtcag
      421 gccgctttgc cgcctggctg atcgagcagg gccagggcca ttgccacgac tccttccagc
      481 agcgggcgct cgggccgcgc ccgtgccagg acgcgaacgc ccatcagcgt gccgagaagg
      541 tgccgggcga gatcttctgc ggccagggcg cgtgaaatcg tgccggcctc ctggccggct
      601 tcgacgcagt cgaagaaaaa gcgctccagg cgcttgagga cgccggcgat ggtttgctgg
      661 aactccgggt cgtgcggcgc catttccagg gcggagttca ccagcatgca tcccttgtgc
      721 tggcggtcgt tcaatgagcg gctcaggata tcgtcgaaaa atgcgtcaat cgcctcgcgg
      781 gggggcagcg ctgcgcagcg ccgcaggcgc gccgcgatgc tgtgctccac gtagcgatcg
      841 agcgcggtcc ggaaaagccc gagcttgtcg ccgtaggcgt tgtagaggct ggcggcggta
      901 atgcccgtcc cattgacgag gtccttgacg gacgtcgatt cgtagcctcg gctccagaag
      961 caatggaccg ccgcatccag gactacggcc tcgtcgaatt ctcttggtct tgccatctcg
     1021 gcacccgatc ccttatggcc caatggcctt gccacgcttg gcatggcggc ctattataga
     1081 acgagcatac taaaactaaa aaacaccaaa ggagccaccc atgatccgtt tctatttcca
     1141 tcccacgccg aatcccgcaa aggtcgcgct cttgctcgaa gagacgggac tgccctacga
     1201 agtggtcccg gtcgatacca gcaagggcga gcagcatgag ccggggttcc gcgcggtcaa
     1261 tccgaacggc aaggtgcccg cgatcatcga caccgaaggc cctggcggga aagaggccag
     1321 ggtcttcgac tccacggcca tcctgctcta cctcgccgag aagaccggca agttcctcgg
     1381 cgccggggag gaccggccgg aactgctatc gtggctcctg ttcctcgcct ccggcctcgg
     1441 cccgttctcc gggcaggccg tgcatttcca gtacgcggcg ccggcgggcc tggactatgc
     1501 cgtgaaccgc tatcggcgcg aagccgagcg gcactatcaa gtcttggacg atcacctcga
     1561 aggccggaca tacattgtcg gcgagaccta tacaatcgcc gacatatcgg cctggggctg
     1621 gctcgaccgc gccgcgcgcg tgcgcaaggg cgaggacgac ccgctcgggc cgtttcccaa
     1681 tctcaagcgg ctcttcgaga cggtcgatgc gcggccggcc gtcgcccgcg cccgcgccgt
     1741 cggccaggat cacgcgttca agaagatcaa cgacgaagag accaagcgcg cgctcttccc
     1801 gtccaactac cccccggccg cttgagcggc cgcaaccgat tgcggagaaa acatggcgga
     1861 ttcccccgac tacacccccc cgaaagtatg gacctggacc cagcccaacg gcggcgcctt
     1921 tgccagcacc aaccggccgg tcgccggacc gacgcatgac aaggaactgc ctgtcggccg
     1981 gcacccgctc cagctctact cgctgggcac gccgaacggc atcaaggtca ctatcctgct
     2041 ggaagagctg ctggccctgg gccatgccgg cgccgagtac gacgcctggc tgatcaagat
     2101 cggcgatggc gaccaattcg gcagcggttt cgtggcggtc aacccgaatt cgaagatccc
     2161 ggcgctgctg gaccgcagcg gcccgaagcc gatccgcgtg ttcgagtcgg gatccatcct
     2221 gctgtacctg gcggaaaagt tcggcgcctt cctcccgaag gacgtcgcgg cgcgcaccga
     2281 aacgctgaac tggctgttct ggcagatggg cagcgctccc tacctgggcg gcggcttcgg
     2341 ccacttctat gcctatgcgc cgaccaagat cgaatatgcg atcgaccgct tcgccatgga
     2401 ggttaagcgc cagctcgacg tgctcgaccg gcgcctggcc gaaagcgcct atctggccgg
     2461 cgatgactac acgatcgccg acatcgcggt cttcccctgg tacggcggac tcgtcaaggg
     2521 ctggcagtac ggggcggcgg aattcctgag cgtgcaggac tacaagcacg tgcagcgctg
     2581 ggccgacgcc atcctggcgc ggccggcggt caagcgcggc cgcatggtca accgcgtgag
     2641 gggcgaaccg tcggagcaac tgcacgaacg ccacgacgcc agcgatttcg acaacaagac
     2701 gcaggacaag ctcgcggggg cgtaatcatg aagatcgaga acaagcgcat cctgatcacc
     2761 ggaggatcga gcggcatcgg tttcgcgctc gctcgcgcac tgctcgccaa gggcgcgcgc
     2821 gtggccatca ccggccggcg tcccgacgcc ctcgcgaagg cgcttcggga gctgcgggag
     2881 atcagcccgt ccgtctcgat cattaccgcg gacgtaacga cgccggacgg tcgcggggcg
     2941 acgatcgggc aggccatcgc cgcgcttggc ggtctcgaca tcctgatgaa caatgccggc
     3001 ggcgtcaggg ctggacgtct tgaaaacacg cccgaagccg aattgcaggc catgatcgat
     3061 gtcgacctcg tcgcgccggt cctcttgacg cgcgccgcgc tgccagccct gcgcgcgagc
     3121 ggcgatgcaa tggtcgtgaa catttcctcc ggcatcgcct tgataggcgc gccgttctac
     3181 gcggcttatg ccgcgaccaa ggccggcctg gcccggttcg gcgagtcgct ccggcgcgaa
     3241 tggaaaggcg agggcatcca tgtgctgacc gcctatcccg gcggcaccga cacgccgatg
     3301 atgcaatcga accgtgccgg gccggagctc ggtttctcgc gcgagccggc ttccactgtt
     3361 gccgatgcca tcgtcgaggg catcgaggcc aatgccttcg aggtcgtccg cggcggcgag
     3421 gcgcgggccc agatgatcgc gctcaaccgc gacaatccgg cggctgtgga cgagcggttc
     3481 ctggggttga aaccagccct cgaagcggcg gtgaaagacc actcggcgct gtgacgcgcc
     3541 gccagagtgg gaatccgcgt ggcggcgcca ggctatcact gccagtacta ggataatagg
     3601 ttccgttgta ctagcttgaa gctgtaggca taataggctg gagacattcc tccacctatt
     3661 aaaagagcgt agtcatggcc cagactctct acgacaaact ctggcagtcc cacgtcgttc
     3721 atcaagaatc cgacggcacc tgcctgctct acatcgaccg ccacctgctg cacgaggtca
     3781 ccagcccgca ggccttcgaa ggcctgaccc tggccggccg caagccctgg cgcatcggcg
     3841 ccaacctggc cgtggccgac cacaacgtgc ccacggtcac ggcccatcgc gcccagggca
     3901 tcgacgatcc cgtctccaag ctgcaggtcg acacgctcga cgacaattgc ggcaagttcg
     3961 gcatcaccga attcaagatg aacgacctgc gccagggcat cgtgcacgtc atcggccccg
     4021 agcagggcgc cacgctgccc ggcatgacgg tggtctgcgg cgactcgcac accagcacgc
     4081 acggcgcgct ggccacgctg gccttcggca tcggcacctc cgaggtcgag cacgtgctgg
     4141 ccacgcagac gctgctcatg aaaaagaaca agagcatgct catcaaggtc gagggcgagc
     4201 tgcccttcgg ctgcacggcc aaggacgtgg tgctgcacat catcggcatc atcggcaccg
     4261 cgggcggcac gggccatgcc atcgaattcg ccggcagcgc catccgcgcg ctgtcgatcg
     4321 aaggccgcat gaccgtgtgc aatatggcca tcgaggcggg cgcgcgctcg ggcatggtgg
     4381 ccgtcgacga caagacggtc gactacttca agggccgccc cttcgcgccc accggcgtgc
     4441 tgtgggacca ggccgtgtcc tactggcgca ccctgcacag cgatcccggc gccaccttcg
     4501 accgcgtgat cgaagtcgac gcccgccaga tcaagccgca agtcacctgg ggcacttcgc
     4561 ccgaaatggt gttgcccgtc gacggccggg tgcccgatcc cgaccgcgaa aaagacgacg
     4621 tgcgccgcag cggcatggag cgcgccctgc aatacatggg cctcacgccc aacacgccgc
     4681 ttaccgacat ccgcgtcgac cgcgtcttca tcggctcctg caccaactcg cgcatcgaag
     4741 acctgcgcgc cgccgcttcc atcgcgcgcg gccgccgcgt ggccgccaat gtaaaagaag
     4801 ccatcatcgt gccgggctcc ggcctgatca agcagcaggc cgagagcgaa ggcctggaca
     4861 agatcttcat cgccgcgggt ttccagtggc gcgaaccggg ctgctccatg tgcctggcca
     4921 tgaacgccga ccgcctcgag cccggcgaac gctgcgcctc cacgtcgaac cgcaacttcg
     4981 agggccggca agggcagggc ggacgcaccc atctggtcag ccccgccatg gccgccgccg
     5041 ccgccgtggc cggccatttc gtcgacgtcc gcacgctgcg ctgaacgcca accaggagca
     5101 catcatgcaa gcattcacct cccacgaagg cctggtggcg ccgctcgacc gcgagaacgt
     5161 cgacaccgac ctcatcatcc ccaagcaatt cctcaagtcg atcaagcgca ccggcttcgg
     5221 ccccaacctg ttcgacgaac tgcgctacct cgaccacggc gagccgggca tggacaacag
     5281 ccgccgcccg ctcaatcccg acttcgtgct caaccagcag cgctaccagg gcgcttccat
     5341 cctgctggcc cgcaagaact tcggctgcgg ctccagccgc gagcacgcac cctgggcgct
     5401 cacgcagtac ggttttcgcg ccatcatcgc gccgtcctac gccgacatct tcttcaacaa
     5461 cagcttcaag aacggcctgc tgcccatcat cctgtccgag ttcgaggtcg ccaagctgtt
     5521 cgacgaagtc aaagccttcc ccggctacaa gctcatcgtc gacctgcccg ggcaggtcgt
     5581 tcgcacgccc ggcggccagg ccatgcactt cgacatcgag cccttccgca agcactgcct
     5641 ggtcaacggc ctggacgaca tcggcctgac cctgcaacat gccgacaaga tccgcgtctt
     5701 cgaggccgag cgcctggcca ggcacccgtg gctggaaggc cagccggtgt tgggttgagg
     5761 tgatggagac gacgcaagtc tgccgcgggc gcaggcccgt ggcggcgcct ctgcggcggg
     5821 gtagcccccc gaagggggcc gagggcgcct ggttgcgccc gagcccgacg cagcgaagcc
     5881 gacacgggcc tgcgcccgcg gcagacgttt taagaactca gatagaacac gcaatagagt
     5941 ccaagacatg acgacccatc aaatcgcagt cctccccggc gacggcatcg gccccgaaat
     6001 caccgaacaa gccgtgcgcg tgctcaccgc gctgaacctg tccctcgacc tcaagcaggc
     6061 ccccgtcggc ggcgccgctt tcgaccagtt cgaacacccg ctgccgcccg ccaccctcga
     6121 gctcgccaag caatcccgcg ccacgctctt cggcgccgtg ggcgactgga aatacgacgc
     6181 cctgccgcgc gaattccgcc ccgagcaggc cattctcggc ctgcgcaaga acctgggcct
     6241 cttcgccaac ctgcgcccgg ccatcctcta tcccgagctg gccagcgcct cctcgctcaa
     6301 gcccgagatc gtcgccggcc tcgacatcct catcatccgc gaactcaccg gcgacatcta
     6361 cttcggcacc ccgcgcggcg tgcgcacggc ccccgacggc gccttcaagg gcgagcgcga
     6421 aggctacgac accatgcgct acaccgaccc cgaagtgcgc cgcatcgccc gcgtcggctt
     6481 cgagaccgcc cgcaagcgcg gcaaaaagct gtgcagcgtc gacaaggcca acgtgctgga
     6541 aacctcgcag ttctggcgcg atatcgttat tgaagtggcg cgcgaatacc ccgatgtcga
     6601 gctgtcgcac atgtatgtcg acaacgccgc gatgcagctg gtgcgcaacc cgcgt
//
LOCUS       DASWPO010000230        15614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_410029, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000230 DASWPO010000000
VERSION     DASWPO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15614
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410029"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VGC69_12170"
     CDS             complement(<1..775)
                     /locus_tag="VGC69_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY0296078.1"
                     /translation="MQIAILDDYHGVAERYADWTALGPQAKVRAFREHLPEGPGRAQA
                     LAPFDVIVAMRERMPFPADLIADLPNLKLLITTGMRNNAIDMAACKARDVLVCGAPGS
                     NQAVNATSELAWALILALFKRIPDEHAAMRTGLWQTDMPQVLSGKRLGVLGLGKLGIA
                     TAQVGRAFGMDVVAWSPNLTDERAAQAGFKRVDKHELFATSDVVSLHLVLSERTQHVV
                     DAAALDAMKPTAFLVNTSRAGLVDQDALFAALREGRLAGA"
     gene            complement(847..1623)
                     /locus_tag="VGC69_12175"
     CDS             complement(847..1623)
                     /locus_tag="VGC69_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY0296079.1"
                     /translation="MTPAFLRKLVAVLCTAAAAALAPAVHAADPAAAMMSTAAVSADP
                     AAEAVQALFHERFPDLDVSRVRRTPYGLYEVQVGMDMVYTDEKVSWVLQGQLVDAMTR
                     RDVTRESLAALGDIPFDKLPLALAVKQVKGNGKRKVAIFEDPNCIYCKQLRHTLQQVS
                     DVTIYTFLYAILAPDSADKSRTIWCSKDPAGAWDDWMLHGKMPASANCDAPIDKVLAL
                     GKQLMVRGTPTLYFGDGTHTSGALPLNMLQARLDGRDAQQ"
     gene            complement(1649..2818)
                     /locus_tag="VGC69_12180"
     CDS             complement(1649..2818)
                     /locus_tag="VGC69_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HEY0296080.1"
                     /translation="MKPQILICGAGIVGLSCALALARRGQRVAVLAPRNEVPAAQPDA
                     YHPRVYAISPASQRLLDELGVWQMLPAARIAPVQAMEIHGDGDGRVDLHAWQAAQENL
                     AWIVESGEIERVLIQAARMVGIEWIDDRCEGWREGEVQTAAGRRLQAELFVGADGAAS
                     TLREAAGLRHDSRSYQDVGLVAHLDAERPHLGTALQWFRDDGVLALLPLPDTAAGPQV
                     SMVWSMREARAQALLGLPRPEQAAELERLLAPAAQGRLGALRARGAVHGFPLTLDQAQ
                     MVGPGIALVGDAAHRVHPLAGQGLNLGLGDVAALVRTVAQRENFRSAGDPRVLRRYQR
                     ARAEPVLAMRLATDGLHRLFGAQAAPLAWLRNAGMRWVDALPFVKRQLIEGASRN"
     gene            complement(2815..4113)
                     /locus_tag="VGC69_12185"
     CDS             complement(2815..4113)
                     /locus_tag="VGC69_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEY0296081.1"
                     /translation="MKRLTRCLCLALVSAVLAACSTTSELPPATSSSGAAAIAAASQE
                     GPLTVPPLSALPETPPRPLSGKFARAAWADMPGWNDDDLSDFWAVFLRDCRGLVRPVS
                     GSLALPARAAPRAWQPVCAAAASQTLNDPAAVRHFLQAWLQPWRLNGPDNQAASNLVT
                     GYYEPVVNGSRQRGGPYQWPLYAVPGDLLTIDLGSVYPELAGKRVRGKLEGQRVVPYD
                     TRAAIESSDHKPPVLVWIDDPVDNFFLQVQGTGRVRLADGPDAGQTLRLAYADHNGQP
                     YMSIGRWLADHGQMTADQASMQNIRAWARRNPSRVQEMLSANPAVVFFREEKLTDASL
                     GPNGAYGLPLTPGRSVAVDAHFVPLGTPVFLATTQPGSAAPLQRLVLAQDTGTAIRGA
                     ARTDFYWGSGDEAGQQAGRMKQRGQMWLLWPKQAGEPSAR"
     gene            complement(4137..4511)
                     /gene="apaG"
                     /locus_tag="VGC69_12190"
     CDS             complement(4137..4511)
                     /gene="apaG"
                     /locus_tag="VGC69_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY0296082.1"
                     /translation="MKPYDLTVAITPRFVPEQSDPGQQQYLFAYTVRITNSGEHPVQV
                     ISRHWIITDGNQQVQEVRGLGVVGQQPLLAPGETFEYTSGCPLPTPVGTMKGIYHCVG
                     ENGIPFEVPIAEFLLAMPRTLH"
     gene            4560..5276
                     /gene="rpe"
                     /locus_tag="VGC69_12195"
     CDS             4560..5276
                     /gene="rpe"
                     /locus_tag="VGC69_12195"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0296083.1"
                     /translation="MPSETPNAMSTTRIAPSILSADFARLGEEVRNVAAAGADWIHFD
                     VMDNHYVPNLTIGPMVCAAIRPHVQIPIDVHLMVEPVDEIVPLFAKAGADLITFHPEA
                     SRHVDRTLSLIHDSGCKAGLVFNPATPLSYMDYVMDKLDVVLLMSVNPGFGGQAFIPT
                     TLEKLRQARARLDRWMQGGGQPILLEVDGGVKIDNIAEIRAAGADTFVAGSAIFGKPD
                     YPAVIRSLREQIARGETLSA"
     gene            5279..5986
                     /locus_tag="VGC69_12200"
     CDS             5279..5986
                     /locus_tag="VGC69_12200"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY0296084.1"
                     /translation="MAPESSPPPLLAALLDLDGTLVDSIPDLAHAANAMRVELGMPPL
                     GLDVLATFVGKGMDNLVRRTLAGTLLNGEPDAALFTQGRASFRHHYGLINGDKATVYD
                     GVVEGLRTMQDQGLQLAVVTNKPTEFTGPLLARTGLAGFFPVIVCGDTCARRKPDPDQ
                     VLHACDRLGVPPAQAVMIGDSMNDALAGRAAGTRVLAVPYGYNEGQDVRTLDVDGIVP
                     RLTDAAQVIARWNAAAG"
     gene            6270..7790
                     /gene="trpE"
                     /locus_tag="VGC69_12205"
     CDS             6270..7790
                     /gene="trpE"
                     /locus_tag="VGC69_12205"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY0296085.1"
                     /translation="MTELEFNALAAQGYNRIPLVAETYADLDTPLALYLKLAHGGPRQ
                     GKMTCLMESVVGGERFGRYSFIGLPARTVIRASGHATEVLTDGVVAETHRGDPLAFIE
                     QYQQRFKVALRPGMPRFCGGLAGYFGYDTVRHMEPRLGPALKPFPAGMEEGTPDLMLM
                     HVDELVIVDNLAGRIYLMIYADPAKPESYSRAQQRLLDLRAQLRKPVDIPYSHASMVT
                     EERRDFKKNDYLAAVRRAKEYIAAGDLMQVQVGQVIAKPFRDAPLSLYRALRSLNPSP
                     YMYFWNFGDFQVVGASPEILVRQESIVQDDEPRSQITIRPLAGTRKRGATPEEDAALA
                     AELKADPKEVAEHVMLIDLARNDVGRVSEPGTVKVTDTMAIERYSHVMHLVSNVAGTL
                     KPGMSSMDVLRAAFPAGTLTGAPKVRAMELIDELEPVRRGLYGGAAGYLSYGGEMDLA
                     IAIRTGVIKNGTLYVQAAAGIVADSNPEAEWAETEAKARAVLRAAEQVQHGLDAPL"
     gene            complement(7860..8006)
                     /locus_tag="VGC69_12210"
     CDS             complement(7860..8006)
                     /locus_tag="VGC69_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296086.1"
                     /translation="MGLWCNEFGCRADAGYSCGRHAALAALRFPMAHVTLITRERQLI
                     KAGG"
     gene            complement(7990..8805)
                     /locus_tag="VGC69_12215"
     CDS             complement(7990..8805)
                     /locus_tag="VGC69_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296087.1"
                     /translation="MEKPARYNLADDVRRRLEEEINDGRLMPGDALDERQLAKRFEVS
                     RTPIREAIHQLAAQGLVRAIPRQGVYVARMSIKELLAMFELQAELEGLCARFAARRMT
                     TDQRQALAAIYARCKESAENRDIRRYAQENADFHETIYRGSHNRYLAEQARTIRRRTE
                     IYRQNTFQREGRIPASLEEHGKVLEAILRGDDAEACVAMRDHISVGGTGFAEFISTLP
                     DEMLESHEQAYPIARAPSPGASADGAPAAKRPAAKKTGTRRPKAVAYKNGALV"
     gene            8955..9908
                     /locus_tag="VGC69_12220"
     CDS             8955..9908
                     /locus_tag="VGC69_12220"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEY0296088.1"
                     /translation="MSMLLPQSRTMDFSEVPVIDIGPLLEGVRMDATIEAIGAACSNV
                     GFLYVKNHGIAQPVIDAMVLQMRRFFALPESRKMESSMEKSTQFRGFLPMKSGGKKVA
                     INHREGYIMREDRPLDPAYPLDGPNVWPPDMPAFRDAMMAYFKEAEHLSRCLLQGFSL
                     ALGLPAGQLAGMFTHPTTQLKLNYYPAQDRPEKEDEIGIVAHADSGAFTILWQDDVGG
                     LETVNGNGEWVVVPPIPGTFVINIGDILQIWSNGRFSSTPHRVINRYGTDRFSVPLFV
                     HPDPDTLVRPLIGAVAPDYVPFVSGEYQEGVYDRLYPRMEA"
     gene            9905..10768
                     /locus_tag="VGC69_12225"
     CDS             9905..10768
                     /locus_tag="VGC69_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0296089.1"
                     /translation="MMARIQVAPGVALHCEATGAGVPVVFLHEFAGDHRTWEPQVRHF
                     SRSHRCVTFSARGYPPSDIPSDPALYSQDIARRDALAVMDALEIERAHIVGHSMGAYT
                     ALHLGISNPERCLSVTAAGCGWGSDPADTPKVVQTCTDIARLFRDNDMATAAAQYARA
                     PMRLTFIAKDPRGFAEFEQMLAQHSGPGSALVMEQVQARRPSLGEMAPALQAMEVPLL
                     VIVGDEDFPCIEGSLLLKRTVPSAALLMLPRTGHTVTSEEPAAFNAALAELFCAAESG
                     TWMRHHASSGA"
     gene            10772..11551
                     /locus_tag="VGC69_12230"
     CDS             10772..11551
                     /locus_tag="VGC69_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0296090.1"
                     /translation="MAGDILIDIDGGIAILRLSSPSRRNAISASMWRTLRNFAVQANR
                     MDDLRAVVVRGDGERIFSAGADIGEFDAARSGADTRAYDDLVESACAAVEAIDRPTLA
                     AIAGPCVGAGVSLAASCDLRIASQDASFSVPAARLGLGYDPRGVARLMRVFGPALTRS
                     LLYTADRIAVRDAGASAILRIAESAQQVAADALVLAQRIAANAPLTLRAAKAAVRAAE
                     RGEAACMQAALEAAALADASADYTEGRKAFAEKRPPNFRGT"
     gene            11554..13104
                     /locus_tag="VGC69_12235"
     CDS             11554..13104
                     /locus_tag="VGC69_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0296091.1"
                     /translation="MASHNLVHHLYGGRTPDSIAISRPGHAPLSTAELRDGVGRYAAC
                     LEQLGLRRGDRVSLKLAKSVDALMLAHGCVQLGAIIHPISTDYTLEETEYLLNDVQPS
                     LIVCETAELERLRAIAPAGTQLQDLGQDGHGGLRGMLRETAPRRDIEPVAPDDIAAIL
                     YTSGTTGRPKGAQITHRNLTECAQALVSAWQITARDRLLHFLPMSHAHGLLTAVNTTL
                     VGGAALLFLERFDLKMVIAALKDTTVMLGVPTHYARLMREPVFAASLPADFRLAISGS
                     APLPLELAQAFSSATGYDLYQRYGATETSIVAAVPADRENRVGWVGWPLPGVQVRVQC
                     DDGAHHGRAATGSLETRGHNVFSGYWRRPEADSEAFTDDGWFITGDIAEIDEQGCVRI
                     LGRKKEIIISGGLNVYPLEVENAIVSLPGIAEAAVFGVPHPDFGEAGVAAVRLEPDGE
                     LDETAVAQALRGKLAPYKIPKRFVAVEQIPSNGNGKISKTELRERFRNMFSDPAASGR
                     TSYNSHKE"
     gene            13109..13918
                     /locus_tag="VGC69_12240"
     CDS             13109..13918
                     /locus_tag="VGC69_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0296092.1"
                     /translation="MDLQLKGKTALVTGGSEGIGKAIAWALAKEGVDVAICARRKKPL
                     EAAAAEIAKATGRKIVAIAADLTKSEDAERFIKEGAAALGRIDIMINNAGSAPGGVIE
                     TLTEADWEQALQLKFMGYVRCLRYALPIMVTQGGGRVVNVIGNDGVKPSYWEITPGAA
                     NAAGQNLTMSLAGQYGKHNISFCAVNPGPVRTERWAGLVQAMSRDMGISYEEADKLAP
                     SAIPLGRIAEPEEVANLVAMMASPLSHFINGTMIEIDGGQEKVLMDQIRDR"
     gene            13936..15126
                     /locus_tag="VGC69_12245"
     CDS             13936..15126
                     /locus_tag="VGC69_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0296093.1"
                     /translation="MDAHRMALPDAPCDAFGAFVALAAGRPAAMGPLAGVRLAVKDNI
                     AVQGLPYTAGLPLFQDRLGPADAAAVRTLQAAGAVVCGVARTDAGGFGVATPRVRNPA
                     YPRHIAGGSSGGPAAAVAAGLADLGLGTDTGGSARIPAACCGLYGFKPSHGAISTDGV
                     WPLAPELDTVGLMARDLDLIRRAYAALAGRDADRGRAPRELVIGVDAGRLAHTDRTVA
                     AAFLRTVDAQRDRFKQVRLLHLPGRGIVQRIHAACVLLAARDIYCGIDRDFSDARYGP
                     TAGAALRAALLLDAAQARRAREDAAGIRQEWEQSMRDVDYVLTPTLAVPRPLAGSRHA
                     RLLGRQIPLVHALTAETALANLVGAPAISIPCGRSLAGLQLSGRAGCDGELIEAAAAF
                     SAPR"
     gene            complement(15140..>15614)
                     /locus_tag="VGC69_12250"
     CDS             complement(15140..>15614)
                     /locus_tag="VGC69_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296094.1"
                     /translation="DEPTSALDVSVQAQILNLLQDLREEFGLTYLLISHNLAVVEHVA
                     THVAVMYLGRIIEYGTTVDVFNRPRHPYTKALLASVLTPVPGIGVPDPGLRGQFPNPI
                     APPSGCAFHPRCAQAMARCSETVPLLRDGCACYLYPAPAAADRSVQDLGRTIAIA"
BASE COUNT         2441 a         5450 c         5375 g         2348 t
ORIGIN      
        1 cggcgccggc caggcggcct tcgcgcaggg cggcgaacag ggcgtcctgg tccaccaggc
       61 cggcgcgcga ggtgttcacc aggaaggcgg tgggcttcat ggcgtcgagc gccgcggcgt
      121 cgaccacgtg ctgcgtgcgc tcgctcaaca ccaggtgcag gctgaccacg tcggaggtgg
      181 cgaacagttc gtgcttgtcc acgcgcttga agccggcctg cgcggcgcgc tcatcggtga
      241 ggttggggct ccaggccacc acgtccatgc cgaaggcgcg gccgacctgc gccgtggcga
      301 tgcccagctt gcccaggccc agcacgccca ggcgcttgcc ggacagcacc tgcggcatgt
      361 cggtctgcca caggccggtg cgcatggcgg cgtgctcgtc gggaatgcgc ttgaaaagcg
      421 cgaggatcag cgcccaggcc agttcggacg tggcgttgac ggcctggttg ctgcccggcg
      481 cgccgcatac cagcacgtcg cgcgccttgc aggcggccat gtcgatggcg ttgttgcgca
      541 tgccggtggt gatcagcagc ttgagattgg gcaggtcggc gatcaggtcg gcgggaaacg
      601 gcatgcgctc gcgcatggcc acgatcacgt cgaaaggcgc cagcgcctgc gcccggcccg
      661 gcccttcggg cagatgctcg cgaaacgcac gcaccttggc ctgagggccg agagcggtcc
      721 aatcggcgta gcgctcggcc acgccatgat agtcatccag tatcgcgatc tgcattgccg
      781 tctcctttgc ccgggcccat accggattct taacctggaa tacctgacac ccgcctgttg
      841 cgccgcctat tgctgcgcat cgcggccgtc gagcctggcc tgcagcatat tgagcggcaa
      901 ggcgccgctg gtatgcgtgc cgtcgccaaa atacagcgtg ggcgtgccgc gcaccatcag
      961 ctgcttgccc aaagccagca ctttgtcgat cggcgcatcg cagttggcgg aggccggcat
     1021 cttgccatgc agcatccagt cgtcccaggc gccggccgga tccttggagc accagatggt
     1081 gcgcgacttg tcggccgagt ccggcgccag gatggcatag aggaaggtgt agatggtgac
     1141 gtcgctcacc tgctgcaggg tatgccgcag ctgcttgcaa tagatgcagt tggggtcttc
     1201 gaaaatggcg accttgcgct tgccgttgcc cttgacctgc ttgaccgcca acgccagcgg
     1261 cagcttgtcg aacgggatgt cgccgagcgc cgcgaggctt tcgcgcgtca cgtcgcgccg
     1321 cgtcatggcg tctaccagtt ggccctgcag gacccagctc accttttcat cggtgtagac
     1381 catgtccatg ccgacctgca cctcgtacag gccgtagggc gtgcggcgca cgcggctcac
     1441 gtcgaggtcg ggaaagcgct cgtggaaaag cgcctgcacc gcctcggcgg ccggatcggc
     1501 cgacacggcg gcggtcgaca tcatggcggc ggccgggtcg gcggcatgca cggccggcgc
     1561 cagcgcggcg gccgccgcgg tgcacagcac cgccacgagc ttgcgtaaga atgcgggggt
     1621 catcaaaggc tccggttcgg cgccgcgctc agttgcgcga agcgccctcg atcagctgtc
     1681 gtttcacaaa gggtagcgca tcgacccagc gcatgcctgc attgcgcagc caggccagcg
     1741 gcgcggcctg cgcgccgaac aggcggtgca ggccgtcggt ggccaggcgc atggccagca
     1801 ccggttcggc gcgggcgcgc tggtagcggc gcagcacgcg cgggtcgccc gccgagcgga
     1861 aattctcgcg ctgcgcgacc gttcgcacca gtgccgcgac atcgcccagg cccaggttca
     1921 ggccctgccc cgccagggga tgcacgcggt gcgcggcatc gccgaccagg gcaatgcccg
     1981 ggccgaccat ctgcgcctga tccagcgtca ggggaaaccc gtgcacggcg ccgcgcgcgc
     2041 gcagggcgcc caggcggcct tgcgcggccg gggccagcag gcgctccagc tcggcggcct
     2101 gctccggccg cggcaggccg agcagggcct gcgcccgggc ctcccgcatg gaccagacca
     2161 tggacacctg cgggccggcg gcggtgtcgg gcagcggcag cagggccagc acgccgtcgt
     2221 cgcggaacca ttgcaaggcc gtgcccaggt gcggccgttc ggcatcgaga tgcgccacca
     2281 ggccgacgtc ttggtaggaa cgggagtcgt gcctgagccc ggcggcctcg cgcagcgtcg
     2341 aggcggcgcc atcggcgccg acgaagagtt cggcctgcag ccggcggccg gcggcggtct
     2401 gcacttcccc ttcccgccaa ccctcgcagc ggtcgtcgat ccattcgatg ccgaccatgc
     2461 gcgcggcctg gatcagcacg cgctcgatct cgccggattc gacgatccag gccaggtttt
     2521 cctgggcggc ctgccaggca tggaggtcga cgcgaccgtc gccgtcgccg tggatttcca
     2581 tggcctgcac gggcgcgatg cgcgcggccg gcagcatctg ccacacgccc agctcgtcga
     2641 gcaggcgctg gctggccggg gaaatggcat agacgcgcgg atgataggcg tcgggctgcg
     2701 cggccggcac ttcgttgcgc ggggccagca cggccacgcg ctggccccgc cgggccagcg
     2761 ccagcgcgca ggacaagccc acgatgccgg cgccgcagat aaggatttga ggtttcatcg
     2821 cgccgaaggc tccccggcct gcttgggcca caacagccac atctggccgc gctgcttcat
     2881 gcggccggcc tgctgcccgg cctcgtcgcc cgacccccag tagaaatcgg tccgcgccgc
     2941 gccgcggatg gccgtgcccg tgtcctgcgc cagcaccagg cgctgcaggg gcgcggccga
     3001 acccggctgg gtggtggcca ggaacaccgg cgtgcccagg ggcacgaagt gcgcgtccac
     3061 cgcgaccgag cgcccgggcg tgagcggcag gccgtaggcg ccgttcggcc ccagcgaggc
     3121 gtccgtcagc ttttcttcgc ggaagaacac cacggccgga ttggcgctga gcatttcctg
     3181 cacgcgcgac ggattgcgcc tggcccaggc gcggatattc tgcatggagg cctgatcggc
     3241 cgtcatctgc ccatggtcgg ccagccagcg gccgatggac atatagggct ggccgttgtg
     3301 gtcggcatag gccaggcgca gggtctgccc ggcatcgggc ccgtcggcca ggcgcacccg
     3361 gcccgtgccc tgcacctgca ggaagaagtt gtcgaccggg tcgtcgatcc agaccagcac
     3421 cggcggcttg tggtcggacg actcgatggc cgcccgcgtg tcgtagggca cgacgcgctg
     3481 gccctcgagc ttgccgcgca cccgcttgcc cgccagctcg ggatagaccg aacccaggtc
     3541 gatggtaagc agatcgccgg gcaccgcgta caagggccat tggtaggggc cgccgcgctg
     3601 gcgcgaaccg ttgaccaccg gttcgtagta gccggtgacg agattgctgg ccgcctggtt
     3661 gtcgggaccg ttcaggcgcc agggttgcag ccacgcctgc aggaaatggc gcaccgcggc
     3721 cgggtcgttc agggtctgcg atgccgccgc cgcgcaaacc ggctgccagg cgcgcggcgc
     3781 cgcccgcgcg ggcaaggcca ggctgccgct gaccggccgc accaggccgc ggcagtcacg
     3841 caggaatacc gcccagaagt cggacaggtc gtcgtcgttc cagcccggca tgtccgccca
     3901 cgcggcgcgc gcgaacttgc cggacaaggg gcgcggcggc gtctcgggca gcgccgacaa
     3961 gggcggcacc gtcaacggcc cttcctgaga cgccgccgca atggcggcgg cgccggacga
     4021 cgacgtggcg ggggggagtt cggaggtcgt gctgcaggcc gccaggacgg ccgacaccag
     4081 ggcaagacaa aggcaacgcg taagacgctt catgaaaaat cgaatcggcc ccatgctcaa
     4141 tgcagggtac ggggcatggc caacaggaat tcggcgatgg gcacttcgaa cgggatgccg
     4201 ttctcgccta cgcaatgata gatgcccttc atggtaccca ccggcgtggg cagcgggcag
     4261 ccgctggtgt actcgaaggt ttccccgggc gcaagcaggg gctgctgccc caccacgccc
     4321 agcccgcgca cttcctgcac ctgctggttg ccgtcggtga tgatccagtg ccggctgatg
     4381 acctgcaccg ggtgctcgcc ggaattggtg atgcgcaccg tgtaggcgaa caggtactgc
     4441 tgctggccgg gatcggactg ctccgggacg aatcgcgggg tgatggcgac ggtcaggtcg
     4501 tagggtttca cgggggggtc ccggggttgc gctgaggaaa actacaataa gctgtaatta
     4561 tgccctcgga aaccccgaac gccatgtcca ccactcgtat cgcccccagc atcctctccg
     4621 ccgatttcgc ccgcctgggc gaggaagtgc gcaatgtcgc cgccgccgga gccgactgga
     4681 tccacttcga cgtgatggac aaccattacg tgcccaacct caccatcggc cccatggtgt
     4741 gcgcggccat ccgcccgcac gtgcaaatac ccatcgacgt gcacctgatg gtcgagccgg
     4801 tggacgagat cgtgccgctg ttcgccaagg ccggcgccga cctcatcact ttccaccccg
     4861 aggcctcgcg ccacgtggac cgcaccctgt cgctcatcca cgacagcggc tgcaaggccg
     4921 gcctggtgtt caacccggcc acgccgctgt cctacatgga ctacgtgatg gacaagctcg
     4981 acgtggtgct gctgatgtcg gtcaaccccg gtttcggcgg ccaggccttc atccccacca
     5041 cgctcgagaa gctgcgccag gcgcgcgcgc gcctcgaccg ctggatgcag ggcggcggcc
     5101 agcccatcct gctggaggtc gacggcggcg tgaagatcga caacatcgcc gagatccgcg
     5161 cggccggcgc cgacaccttc gtggccggct cggcgatctt cggcaagccc gactacccgg
     5221 ccgtgatcag gtcgctgcgt gaacagatcg cccgcggcga aacgctgagc gcgtgatcat
     5281 ggcgccggaa agctcaccgc ctcccctcct cgccgcgctg ctggacctgg acggcacgct
     5341 ggtcgattcc atccccgacc tggcccacgc cgccaacgcc atgcgggtcg aactgggcat
     5401 gccgcccctg ggcctggacg tgctggccac tttcgtgggc aagggcatgg acaacctggt
     5461 gcgccgcacg ctggcgggca cgctcttgaa cggcgagccc gatgcggcgc tgttcaccca
     5521 gggccgcgcc tcctttcgcc atcactacgg cctgatcaac ggcgacaagg ccacggtgta
     5581 cgacggcgtg gtcgaaggcc tgcggaccat gcaggaccag ggcctgcaac tggccgtggt
     5641 caccaacaag cccaccgagt tcaccggccc gctgctcgcg cgcaccgggc tggccggctt
     5701 cttccccgtg atcgtgtgcg gcgacacctg cgcgcgccgc aagcccgacc cggaccaggt
     5761 gctccacgcc tgcgaccggc tgggcgtgcc ccccgcgcag gccgtcatga tcggcgactc
     5821 gatgaacgac gccctggccg gccgggccgc cggcacccgc gtgctggccg tgccctacgg
     5881 ctacaacgaa gggcaggacg tgcgcacgct ggacgtcgac ggcatcgtgc cgcggctcac
     5941 cgacgcggcc caggtcatcg cgcgctggaa cgcggcggca ggctgaggcc gccgcgccag
     6001 ggcagacgcc ggatgccgtg atatagtctt cgccagtcaa cgagcaaaca gcccgctacc
     6061 catcatgacc gtccgccccg ccgcatccct gcgcaacgcc tcctggcgtt ggtggcgcac
     6121 gccttccggg tggcaatcgg ctcgcctggc actggacgca cccgccgcct agcgcctcgc
     6181 tcgacacaaa agccaagccc ggaacctcga caggaccggg ctttttgttt ggcatgtccc
     6241 ggtcgaaccc tcacccacct ggacccgaca tgaccgaact cgaattcaac gccctggcgg
     6301 cgcaaggcta caaccgcatc cccctggtgg ccgagaccta tgccgacctg gatacgcccc
     6361 tggcgctgta cctgaaactc gcgcacggcg ggccgaggca ggggaaaatg acatgcctga
     6421 tggagtccgt cgtcggcggc gagcgcttcg gccgctactc cttcatcggc ctgccggccc
     6481 gcaccgtcat ccgcgccagc ggccatgcca ccgaagtgct gaccgacggc gtggtggcgg
     6541 aaacccaccg gggcgacccg ctggccttca tcgagcagta ccagcagcgc ttcaaggtgg
     6601 cgctgcgccc cggcatgccg cgcttctgcg gcggcctggc gggctacttc ggctacgaca
     6661 ccgtgcgcca catggagccg cgcctgggcc cggccctcaa gccctttccg gcgggcatgg
     6721 aagaaggcac gcccgacctg atgctgatgc acgtggacga gctggtgatc gtcgacaacc
     6781 tggccggccg catctacctg atgatctatg ccgacccggc caagcccgag agctacagcc
     6841 gcgcccagca gcgcctcctg gatctgcgcg cccagttgcg caagcccgtg gacatcccct
     6901 acagccatgc cagcatggtc accgaagaac ggcgcgactt caagaaaaac gactacctgg
     6961 ccgccgtgcg ccgcgccaag gaatacatcg ccgccggcga cctgatgcag gtgcaggtgg
     7021 gccaggtgat cgccaagccc tttcgcgacg cgccgctgtc gctgtaccgc gcgctgcgtt
     7081 cgctcaatcc ctcgccctat atgtacttct ggaatttcgg cgacttccag gtggtgggcg
     7141 cctcgcccga gatcctggtg cgccaggaaa gcatcgtcca ggacgatgag ccccgctcgc
     7201 aaatcaccat ccgccccctg gccggcacgc gcaagcgcgg cgccacgccg gaagaagacg
     7261 ccgcgctggc cgccgagctc aaggccgatc ccaaggaagt cgccgagcac gtgatgctga
     7321 tcgacctggc gcgcaacgac gtgggccgtg tgagcgagcc gggcacggtc aaggtgaccg
     7381 acaccatggc catcgagcgc tattcgcacg tgatgcacct ggtgtcgaac gtggccggca
     7441 cgctcaagcc cggcatgagc agcatggacg tgctgcgcgc ggcgttcccg gccggcacgc
     7501 tgaccggcgc ccccaaggtg cgcgccatgg aactgatcga cgagctcgag cccgtgcgcc
     7561 gcggcctgta cggcggggcg gccggctacc tgagctacgg cggcgagatg gacctggcca
     7621 ttgccatccg caccggcgtc atcaagaacg gcacgctcta tgtgcaggcc gcggccggca
     7681 tcgtggccga ctccaacccc gaggccgaat gggccgagac cgaggccaag gcccgcgccg
     7741 tgctgcgcgc cgccgaacag gtgcagcacg gcctggacgc gcccctctga atcgccccgg
     7801 acggcaaggc tcgcggtgga tggccagcgt gcagatgggg gctctcgcga gggatatcgt
     7861 cacccgccgg ccttgatgag ctggcgctcc cgcgtgatga gcgtgacatg cgccatggga
     7921 aagcgcaggg cggccaacgc ggcatgccgg ccacatgaat agccggcgtc ggcgcggcag
     7981 ccgaattcat tacaccaaag ccccattttt gtatgccaca gctttgggcc gccgcgttcc
     8041 cgtcttcttg gccgcgggcc gctttgccgc gggcgccccg tccgcgctgg cgccgggcga
     8101 cggcgcgcgg gcaatgggat aggcctgctc gtgggattcg agcatctcgt caggcagggt
     8161 ggagatgaac tccgcgaagc cggtgccgcc caccgagata tggtcgcgca tggcaacgca
     8221 agcctcggcg tcgtcgccgc gcaggatcgc ttccagcacc ttgccgtgct cttccagcga
     8281 cgccgggatc cggccttcgc gctggaaggt gttctggcgg tagatttcgg tgcggcgacg
     8341 gatcgtgcgg gcctgttcgg ccaggtagcg gttgtggctg ccgcggtaga tggtttcgtg
     8401 gaagtcggca ttttcctgcg catagcggcg gatatcgcgg ttttccgcgc tttccttgca
     8461 gcgcgcgtag atggccgcca gcgcctggcg ctggtcggtc gtcatgcggc gtgcggcaaa
     8521 gcgcgcgcac aagccctcca gctcggcctg cagctcgaac atggccagca gttccttgat
     8581 ggacatccgg gccacataca cgccctggcg gggaatcgcc cgcaccaggc cctgcgccgc
     8641 gagctggtgg atggcttcgc ggatgggcgt gcgcgacacc tcgaagcgct tggccagttg
     8701 gcgctcgtcg agcgcatcgc ccggcatcag gcgtccgtcg ttgatctcct cttccagccg
     8761 ccggcgcacg tcgtcggcca ggttgtagcg ggcaggtttt tccatatgcg tccacaaaaa
     8821 ttataacgac gccctcttga ataccaacag tgttgtttgg tattattgtt tccagattga
     8881 ggacaccctg agcatatcag gatttcgcga cgtacacagg ctacgccgcc ggggcaagag
     8941 caaggagcca ttcgatgagc atgttgctgc cgcagtcacg caccatggat ttttccgaag
     9001 tgccggtgat cgacatcggg cctctattgg agggggtccg gatggacgcg acgatcgagg
     9061 ccatcggcgc ggcctgttcg aatgtcggct tcctgtacgt gaagaaccac ggcatcgcgc
     9121 agccggtgat cgacgcaatg gtgctgcaga tgcggcgctt cttcgccttg ccggaatcgc
     9181 gcaagatgga atcgtcgatg gagaaatcca cccagttccg cggttttctg cccatgaaga
     9241 gcggcggcaa gaaggtggcc atcaatcacc gcgaaggcta catcatgcgc gaggaccggc
     9301 cgctcgatcc ggcctatccg ctggatggcc ccaatgtgtg gccgcccgac atgccggcgt
     9361 tccgcgacgc gatgatggcc tatttcaagg aggccgagca cttgtcgcgc tgcctgctgc
     9421 agggcttttc gctggccctg ggcctgcccg ccggccaact ggccgggatg ttcacgcatc
     9481 ccaccaccca gttgaagctg aattactacc cggcgcagga tcgtcccgaa aaagaggacg
     9541 agatcggcat cgtggcgcac gcggattccg gcgccttcac catcctgtgg caggacgacg
     9601 tgggcgggtt ggaaaccgtc aacggcaatg gcgaatgggt ggtcgtgccg ccgattccgg
     9661 gcacgttcgt catcaatatc ggcgacatcc tgcagatctg gtccaacggg cggttctcct
     9721 ccacgccgca ccgcgtcatc aaccgctatg gcaccgaccg cttctcggtg ccgctattcg
     9781 tgcatcccga cccggatacg ctggtgcgcc cgctgatcgg cgcggttgcg ccggactacg
     9841 tgcccttcgt cagcggcgag taccaggaag gcgtgtacga ccgcctctat ccgcgcatgg
     9901 aggcatgatg gcgcgcatcc aagtcgcacc cggcgtggcg ctgcactgcg aggccaccgg
     9961 cgccggcgtg ccggtggtat tcctgcacga atttgccggc gaccatcgca cctgggaacc
    10021 gcaggtgcgc catttttcgc gctcgcaccg ctgcgtgacc ttcagcgcgc gcggctatcc
    10081 gccgtccgac ataccgtccg atccggccct gtacagccag gacattgccc gccgcgacgc
    10141 gctggccgtc atggacgccc tggaaatcga gcgcgcccac atcgtcggcc actcgatggg
    10201 ggcatacacg gccctgcatc tcggtatatc gaacccggag cgctgtcttt cggtcacggc
    10261 cgccggctgc ggctggggtt ccgatccggc cgacacgccc aaggtggtgc agacctgcac
    10321 cgacatcgcc aggctgttcc gcgacaacga catggcgacg gcggccgcgc agtatgcgcg
    10381 cgcgccgatg cggctgacct tcatcgccaa ggatccccgc ggcttcgccg aattcgagca
    10441 gatgctggcg cagcactcgg ggccgggctc ggcactggtc atggagcagg tgcaggccag
    10501 gcgcccgtcg ctgggcgaga tggcgcccgc cctgcaggcc atggaagtgc cgctgctggt
    10561 catcgtgggc gacgaggact tcccctgcat cgaaggcagc ctgctgctca agcgtaccgt
    10621 gccgagcgcc gccctgctga tgctgccgcg caccggccac accgtcacca gcgaagagcc
    10681 ggccgccttc aacgccgccc tggccgaact gttctgcgcg gccgagtccg gcacctggat
    10741 gcgccaccac gcgtcgtccg gggcatagca gatggcgggc gacatcctca tcgacatcga
    10801 cggcggcatc gctatcctgc gcctgtccag cccatcccgg cgcaacgcga tttcggcgtc
    10861 catgtggcgc accctccgca acttcgccgt gcaggccaac cgcatggacg atctgcgcgc
    10921 ggtggtcgtg cgcggcgacg gcgagcgcat cttctcggcc ggggcggaca tcggcgagtt
    10981 cgacgccgcg cgcagcggcg ccgacacccg cgcctacgac gacctggtcg aatccgcctg
    11041 cgcggcggtc gaagccatcg accggccgac gctggcggca atcgccggcc cgtgcgtggg
    11101 agcgggcgtc agcctggcgg ccagctgcga cctgcgcatc gcatcgcagg acgcctcttt
    11161 ctcggtgccg gccgcgcggc tcgggctcgg ctacgacccg cgtggcgtgg cgcggctgat
    11221 gcgggtcttc ggcccggccc tcacgcgcag cctgctctac acggcggacc ggatcgcggt
    11281 gcgcgacgcc ggcgcgtcgg ccatcctgcg gatcgccgag tccgcccagc aagtggccgc
    11341 cgatgcgctg gtattggcgc agcgcatcgc cgccaacgcg ccgctgacat tgcgcgcggc
    11401 caaagcggcc gtgcgcgcgg ccgagcgcgg cgaggcggcc tgcatgcagg ccgcgctcga
    11461 ggccgccgcc ctggccgacg ccagcgccga ctacaccgag ggccgcaaag cgttcgcgga
    11521 aaaacgtccg cccaattttc gcggaacgta gccatggctt cccacaatct ggttcaccat
    11581 ctgtacggcg ggcgtacgcc ggactcgatc gccatctcgc gcccgggcca tgcgccgctg
    11641 tcgacggccg agctgcgcga cggggtgggc cgctacgccg cctgcctgga gcagttgggc
    11701 ctgcggcgcg gggaccgcgt ctcgctcaag ctggccaagt cggtggatgc gctgatgctg
    11761 gcgcacggct gcgtccagct cggcgccatc atccacccga taagcaccga ctacacgctc
    11821 gaagagaccg agtacctgtt gaacgatgtg cagcccagcc tgatcgtgtg cgagacggcc
    11881 gagctggaac gactgcgcgc catcgcgccg gcgggcacgc agcttcagga tctggggcaa
    11941 gacggccacg gcggcctgcg cggcatgctg cgcgagaccg cgccgcgccg ggacatcgag
    12001 ccggtggcgc ccgacgacat cgccgccatc ctgtatacct ccggcaccac cggccggccc
    12061 aagggggccc agatcacgca ccgcaatctg accgagtgcg cgcaggccct ggtgtcggcc
    12121 tggcagatca ccgcgcgcga ccggctgctg cacttcctgc cgatgtcgca cgcgcacggc
    12181 ctgctgaccg cggtcaatac cacgctggtg ggcggggcgg cgctgctgtt cctggaacgg
    12241 ttcgacctca aaatggtgat cgcggcgctg aaggacacga ccgtgatgct gggcgtgccg
    12301 acgcactacg ccaggctgat gcgcgaaccc gtgttcgccg cgtcgctgcc ggccgatttc
    12361 cggctggcca tctccggctc ggcgcccctg ccgctggaac tggcgcaggc gttctccagc
    12421 gccaccggat acgacctgta ccagcgctat ggcgccacgg aaaccagcat cgtcgcggcc
    12481 gtgccggccg accgggagaa ccgcgtcggc tgggtgggct ggccgttgcc gggcgtgcag
    12541 gtgcgggtgc agtgcgacga cggcgcgcac cacggccgcg ccgcgacggg cagcctggag
    12601 acgcgcggcc acaatgtgtt ctcgggctac tggcgacggc ccgaggccga cagcgaggcc
    12661 ttcaccgacg acggctggtt catcaccggc gatatcgccg agatcgacga gcagggctgc
    12721 gtgcgcatcc tcggccgcaa gaaggaaatc atcatcagcg gcggcctgaa cgtgtatccg
    12781 ctggaggtcg agaacgccat cgtctcgctg cccggcatcg ccgaggcggc ggtgttcggg
    12841 gtgccgcatc cggatttcgg cgaggccggc gtggcggccg tgcggctcga accggacggc
    12901 gagctggacg aaacagccgt cgcacaggcg ctgcgcggca agctggcccc ctacaagatt
    12961 ccaaaacgct tcgtcgcggt cgagcagatt cccagcaacg gcaacggcaa gatcagcaag
    13021 accgaattgc gcgaacgctt tcgcaatatg ttctcggacc cggccgcatc cggccgaacc
    13081 tcatacaaca gccacaagga gtagtcgcat ggatttgcag ctcaagggaa aaaccgccct
    13141 cgtcaccggc ggcagcgaag ggatcggcaa ggccattgcc tgggcgctgg ccaaagaggg
    13201 cgtggacgtg gcgatctgcg cccggcgcaa gaagccgctg gaggctgccg ccgccgagat
    13261 cgccaaggcc accggccgca agatcgtcgc gatcgcggcc gacctgacca agtccgagga
    13321 cgccgagcgc ttcatcaagg aaggcgcggc ggcgctggga cggatcgaca tcatgatcaa
    13381 caatgccggc tccgcgccgg gcggcgtcat cgagacgctg accgaggccg actgggagca
    13441 ggcgctgcag ctcaagttca tgggctatgt gcgctgcctg cgctacgcgc tgcccatcat
    13501 ggtgacccag ggcggcgggc gcgtggtgaa cgtgatcggc aacgacggcg tcaagccctc
    13561 ctactgggaa atcacgccgg gcgcggcgaa cgccgcgggc cagaacctga cgatgtcgct
    13621 ggctggccag tacggcaagc acaacatcag cttctgcgcg gtcaacccgg ggccggtgcg
    13681 caccgagcgc tgggccgggc tggtccaggc gatgtcgcgc gacatgggga tttcctatga
    13741 agaggccgac aagctggcgc cgtcggcgat tccgctgggg cgcatcgccg agcccgaaga
    13801 ggtggccaac ctggtcgcga tgatggcttc gccgctctcg cactttatca acggcaccat
    13861 gatcgagatc gatggcggcc aggaaaaagt gctgatggac cagatccgag accgctgagc
    13921 ggcgcgcctg ccggcatgga tgcgcatcgc atggccttgc cggatgcgcc ctgcgatgcc
    13981 ttcggcgcct tcgtcgcgct cgcggcgggg cggcccgccg ccatgggccc gctggcaggc
    14041 gtgcgcctgg cggtcaagga caacatcgcc gtgcagggcc tgccgtatac ggcaggcctg
    14101 cccttgttcc aggatcgcct cggcccggcc gatgcggccg ccgtgcgtac gctgcaggcc
    14161 gcgggcgcgg tggtgtgcgg cgttgcccgc accgatgccg ggggcttcgg cgtggcgacg
    14221 ccgcgggtac gcaatccggc ctatccaagg cacatcgccg gcggctcctc gggcggcccg
    14281 gcggcggcgg tcgcggccgg gctggccgac ctgggcctgg gcaccgacac cggcggcagc
    14341 gcgcgcatcc cggccgcctg ctgcggcctc tacggattca agcccagcca tggcgcgatc
    14401 tcgaccgacg gcgtctggcc gctggccccc gagctggaca cggtgggact gatggcccgc
    14461 gatctcgacc tgatccggcg cgcctatgcc gcgctggccg gccgggacgc cgatcgcggc
    14521 cgggcgccgc gcgaactggt catcggcgtg gatgccgggc gcctggcgca caccgaccgg
    14581 accgtggccg ccgccttctt gcggacggtg gatgcccagc gcgaccgctt caagcaggtt
    14641 cgcctgctgc acctgcccgg acgcggcatc gtccagcgca tccatgccgc ctgcgtgctg
    14701 ctggcggccc gcgacatcta ttgcgggata gaccgcgatt tctccgacgc gcgctatggg
    14761 ccgacggcgg gcgccgcgct gcgggccgcg ctgctgctgg atgcggcgca ggcgcgccgg
    14821 gcgcgcgagg acgcggcggg catccggcag gaatgggaac agtcgatgcg ggatgtcgat
    14881 tatgtgctga ccccgacgct ggcggtgccg cgtccgctcg ccggcagccg ccatgcgcgc
    14941 ctgctggggc ggcagattcc gctggtgcac gcgctgacgg cggaaaccgc gcttgccaac
    15001 ctggtgggcg cgccggccat ctccatcccg tgcgggcgca gcctggccgg cctgcagctg
    15061 tccggcaggg ccggctgcga cggcgagttg atcgaggccg cggcggcctt ctcggcgccg
    15121 cgctgaaagc aatcccgtct caggcgatcg ctatcgttcg gcccaggtct tgtacggacc
    15181 ggtccgccgc cgccggcgcc ggatagagat agcaggcgca gccgtcgcgc agcagcggca
    15241 cggtttccga gcagcgcgcc atggcctgcg cgcagcgcgg atggaacgcg cagccggacg
    15301 gcggcgcgat cgggttggga aactgccccc gcaggccggg gtccggcacg ccgatgccgg
    15361 gcaccggcgt cagcaccgaa gccagcaggg ccttggtgta cggatggcgc ggccggttga
    15421 agacgtcgac ggtggtgccg tattcgatga tgcgccccag gtacatgacc gccacgtggg
    15481 tggccacgtg ctccaccacg gccaggttgt ggctgatcag caaataggtc aggccgaatt
    15541 cctcgcgcag gtcctgcagc aggttcagga tctgcgcctg caccgacacg tccagcgccg
    15601 aggtcggctc gtcg
//
LOCUS       DASWPO010000231         6416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2109186, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000231 DASWPO010000000
VERSION     DASWPO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109186"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGC69_12255"
     CDS             <1..370
                     /locus_tag="VGC69_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0296095.1"
                     /translation="SGLAATWGLLVLLWQPVVDYARSYRTASAELAAVLQANVRPGEC
                     VRSQGLGPGQRASFLVFQGLNFPYDPACVLVLQQTTRQSLRDGTAAYSGADAQELWRG
                     GRLADRNEIFRLLRVPPGNP"
     gene            367..1800
                     /locus_tag="VGC69_12260"
     CDS             367..1800
                     /locus_tag="VGC69_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0296096.1"
                     /translation="MTASSHAPAQSLGTTMLNIMRQAWPVLVGQWASIAFGVLDTAMT
                     GHSSPADLAAMSLSASIYITVFVGLMGVVHALIPILAQHYGAQRHHEVGRLWGQGVWL
                     ALGLSVLGGAIMQFPDAWLSMSGNVDPAVRERMASYLEALTLALPAMLVFRTIYALGS
                     AVSRPRVAMLINLAAVLLKVLFNWLLIYGQLGLPAMGATGAGLSTALVAWLGLGAGLW
                     FVTHDRGFAMFRLRLGWPQWHALKELLRLGLPMGGSYLIEVSAFTFMALLVAREGTYV
                     LGGHQIMSNLAALCYMMPMSIGVASSAVTAQSVGAASLAQARRTGWAGLALGLLGALL
                     TSAVVLAGRPYIVAAYTSDAQVAAAASSLVMMMPLFHLWDAFQCINAYLLRAYKVAVA
                     PLLIQTLALAVVGLLGGWWFGFGPGEGGLDGVIAVLMPGARTGAGSLWLMAMIGLGLS
                     ALLLHGWYWRTLRRLGRRAATPDLSGI"
     gene            1802..2581
                     /locus_tag="VGC69_12265"
     CDS             1802..2581
                     /locus_tag="VGC69_12265"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296097.1"
                     /translation="MPNLRLALHDAIPLYFRLYVALEQKILAGEWPEGRPLPSEQELA
                     AQYKVSRVTVRNTMELLKNAALVVRHRGRGTFVNMVTLRKRGQPNFSGLSENSRDFEA
                     TTAIVLHEFGEVDVPEEVSQLLNGAMSGRALRIVRTRHTKAGPFSYSICYVSQPEAAS
                     LDKNELGNLTVLTLLERKGFLASRALQRLTSRAATPEMAGRLGIETGEPLICMKRAVY
                     DRQERLIEYIETFYRPDKFEYRVELSRERGGERPPRWVQAN"
     gene            complement(2775..2960)
                     /locus_tag="VGC69_12270"
     CDS             complement(2775..2960)
                     /locus_tag="VGC69_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296098.1"
                     /translation="MTKSRIALILSASALCFGLAAAPAASFAADSSATAVKVAAKKKV
                     KKAKKAKKAKTAAPAAN"
     gene            complement(3200..3979)
                     /locus_tag="VGC69_12275"
     CDS             complement(3200..3979)
                     /locus_tag="VGC69_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0296099.1"
                     /translation="MSLLAVAGICLVAGAMIGFLGGLLGIGGGLIAIPALALVLGMPQ
                     QLAQGTALVLVLPTVMLSVRKYYQHAQFDLRVVFAGALVSVFFTWVGARLALGIDPRL
                     LRLSFAGFLFCVALFYVVQARRKRRCAAAAASARSAPAFTPRRAAALSVATGTLGGFF
                     SVGGAVLAVPVITTVYRLPQTTAQALALSTVIPGSAIALATYAWAGQTDWHVGLPLAA
                     GSLLFVAAGVRLAHRMAEWKLRAAFALMLFVTVALLIVESR"
     gene            complement(3976..5340)
                     /gene="radA"
                     /locus_tag="VGC69_12280"
     CDS             complement(3976..5340)
                     /gene="radA"
                     /locus_tag="VGC69_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY0296100.1"
                     /translation="MAKSRSIYVCSECGGTSLKWQGKCPHCGAWNALEETVETPQSSA
                     SAAHRYAPLAASTPVRSLADIEARETPRQPTGLEEFDRVLGGGLVPGAVVLIGGDPGI
                     GKSTLLLQALASLSEAVPVLYVTGEESAEQVALRARRLGLTTGKVDLLAEIRLEAIQA
                     AVAEQKPSVAVIDSIQTLYSGELTAAPGSVSQVRECAAQLTRLAKQTGITIVMIGHVT
                     KDGALAGPRVLEHIVDTVLYFEGDTHSSFRLVRAFKNRFGAVNELGVFAMTDRGLRGV
                     ANPSALFLSQHPQQVAGSCVMATQEGTRPLLVEVQALVDASHAPNPRRLTVGLEGNRL
                     AMLLAVLHRHAGVSTYDQDVFVNAVGGVRITEPAADLPVLLAIMSSLRDKPLPRGLVA
                     FGEVGLAGEIRPAPRGQERLREAAKLGFSTALIPKANAPKQPIEGLEIWAVDRLDAAL
                     EKLR"
     gene            complement(5440..6159)
                     /locus_tag="VGC69_12285"
     CDS             complement(5440..6159)
                     /locus_tag="VGC69_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296101.1"
                     /translation="MKDKCVLITGATKGIGWALTQRLADQGCHVVGIARNTEDVDFPG
                     YLYACDLADAGRTEEVLREIREKYPVDAVVNNVGVVSPQPLGSIDLATLYRVLDLNVR
                     VAVQVTQTFVESMKVRRAGRIVNIASRAIHGGYDRTAYSAAKSALVGCTNTWALELAE
                     YGITANAIAPGPIETELFRQNHPAGSEAEKLSLSSIPMKRFGTPADVAAAAAFLLSDE
                     AGYITGQVLGVDGGGALGGRR"
     gene            complement(6186..>6416)
                     /locus_tag="VGC69_12290"
     CDS             complement(6186..>6416)
                     /locus_tag="VGC69_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0296102.1"
                     /translation="GTPITVAGMPTRVVGRVSMDMVAVDLDPVPDAGIGAPVVLWGEG
                     GPSVDEVAQASGTIGYELMCALALRVPVQTLS"
BASE COUNT         1024 a         2208 c         2202 g          982 t
ORIGIN      
        1 cagcggcctg gccgccacct ggggcctgct ggtgctgctg tggcagccgg tggtggacta
       61 cgcgcgcagc taccgcaccg cctcggccga gctggccgcc gtgctgcagg ccaatgtgcg
      121 gcccggcgaa tgcgtgcgca gccaaggcct gggccccggc cagcgcgcct cgttcctggt
      181 gttccaaggg ctcaacttcc cctacgaccc ggcctgcgtg ctggtcctgc agcaaaccac
      241 ccgccaaagc ctgcgcgacg gcacggccgc ctatagcggc gccgacgcgc aggaactctg
      301 gcgcggcggc cgtcttgccg accgcaacga aatcttccgc ctgctgcgcg tgccacccgg
      361 caatccatga ccgcgtcgtc tcacgcgccc gcgcagagcc tgggcacgac catgctgaac
      421 atcatgcgcc aggcctggcc ggtgctggtg ggccagtggg ccagcattgc gttcggcgtg
      481 ctcgatacgg ccatgaccgg ccactccagc ccggccgacc tggccgccat gtcgctgtcg
      541 gcctcgatct acatcaccgt attcgtcggc ctgatgggcg tggtgcacgc gctcatcccc
      601 atcctggccc agcactacgg cgcgcagcgc caccatgaag tgggccgcct gtgggggcaa
      661 ggcgtctggc tggcgctggg cctgtcggtg ctgggcggcg cgatcatgca gtttcccgac
      721 gcctggctgt cgatgtcggg caacgtcgac ccggccgtgc gcgagcgcat ggcgtcctac
      781 ctggaagcgc tgacgctggc cctgccggcc atgctggtgt tccgcacgat ctatgcgctg
      841 ggctcggccg tgtcgcgccc ccgcgtggcg atgctgatca acctggcggc cgttctcctc
      901 aaggtgctct tcaactggct gctcatctac ggccaactgg gcctgcccgc gatgggcgcg
      961 acgggcgcgg gactatcgac cgcgctggtg gcctggctgg gcctgggcgc cggcctgtgg
     1021 ttcgtgacgc acgaccgggg cttcgcgatg ttccgcctga ggctgggctg gccgcaatgg
     1081 cacgcgctca aggagctgct gcgcctggga ctgcccatgg ggggctcgta cctgatcgag
     1141 gtatcggcct tcaccttcat ggccctgctg gtggcgcgcg agggcacata cgtgctgggc
     1201 ggacaccaga tcatgtccaa cctggcggcc ctctgctaca tgatgcccat gtccatcggc
     1261 gtggccagtt cggccgtgac ggcgcaatcg gtcggcgcgg ccagcctggc gcaggcgcgg
     1321 cgcaccggtt gggcgggcct ggcgctgggc ctgctgggcg ccctgctgac ctcggccgtc
     1381 gtgctggcgg gccgccccta catcgttgcc gcctacacca gcgatgccca ggtcgccgcg
     1441 gcggcgagca gcctggtgat gatgatgccg ctctttcacc tgtgggatgc cttccagtgc
     1501 atcaacgcct atctgctgcg cgcctacaag gtggcggtgg cccccctgct gatccagacg
     1561 ctcgcgctgg ccgtggtggg cctgctgggc ggctggtggt tcgggttcgg cccgggtgaa
     1621 ggcgggctcg acggcgtcat cgccgtgctc atgccgggcg cccgcaccgg ggccggcagc
     1681 ttgtggctga tggctatgat aggcctgggc ctgtcggccc tgttgctgca cggatggtac
     1741 tggcgtacgc tgcgccgcct ggggaggcgg gctgccaccc ccgatctctc aggaatctaa
     1801 gatgcccaac ctcagactgg cgttgcacga tgccattccg ttgtatttcc ggctttacgt
     1861 cgcgctggaa cagaagatcc tggccggcga atggcccgaa ggccggcccc tgcccagcga
     1921 gcaggaactg gccgcccagt acaaggtctc gcgcgtgacc gtgcgcaaca ccatggagct
     1981 gctcaagaat gcggcgctgg tggtgcgcca ccgcggccgc ggcaccttcg tcaacatggt
     2041 caccctgcgc aagcgggggc agcccaactt cagcggcctg tccgagaact cccgcgattt
     2101 cgaggccacc acggccatcg tcctgcacga gttcggcgaa gtcgacgtgc ccgaagaggt
     2161 ctcgcaactg ctcaacggcg cgatgagcgg gcgcgccctg cgcatcgtcc gtacccgcca
     2221 cacgaaggcc gggccgtttt cctattcgat ctgctatgtg tcgcagccgg aagccgccag
     2281 cctcgacaag aacgaactgg gcaacctcac cgtgctgacc ctgctcgagc gcaagggctt
     2341 tctggcctcc cgggccctgc agcggctgac ctcgcgcgcc gccacgcccg agatggccgg
     2401 gcgcctgggc atcgaaaccg gcgaaccgct gatctgcatg aagcgcgccg tctacgaccg
     2461 gcaggaacgc ctgatcgaat acatcgagac cttctaccgg cccgacaagt tcgaataccg
     2521 ggtcgagctc tcacgcgaac ggggcggcga acgtccgcca cgctgggtgc aggccaactg
     2581 aaaaaacgga acgaaacgca aggggaagcg gccagaaacg gcctggagac aaaagcttgg
     2641 gagggaaggc ctgagagaag gctttccggc aacccggcgg cccgacccgg agacgacacg
     2701 gacatggagg ccgtgcggcc ggggcgagcc gccggctcgc cttaacaatc gcagaagacc
     2761 tgcgctatcg ctgcttagtt ggcggcggga gcggcggtct tggccttctt agccttcttg
     2821 gccttcttga ccttcttctt ggcggcaacc ttgacggcgg tggccgacga gtcggcggcg
     2881 aacgaagcgg ccggggcagc agccaggccg aagcacagag ccgaagcaga caggatcagg
     2941 gcgatacgcg atttggtcat ggcgagaact cctaagatag tgacgggccc gccggcccgg
     3001 ctgccgagac ctggccaaag tgccagatac ctcatgcata caggcgttgg agtagtcgcc
     3061 tcgataaagg ttcacggtct cgccaaaatt ttttacgctg cttgacgctt gtcctggctc
     3121 gcgccggatg tcatcgcgct tttgttcaac aaccgcgcat accttgcata atttttcaac
     3181 aatgttctca tccgcctgat cagcgggact cgacgatcag caaggccacg gtgacgaaaa
     3241 gcatgagcgc gaaagccgct ctcagtttcc attcagccat gcgatgcgcc agccggacgc
     3301 cggcggccac gaacaggagg ctgccggccg ccagcggcag gccgacatgc caatcggtct
     3361 gcccggccca tgcataggtg gccagggcga tggccgatcc gggaatcacc gtggacaagg
     3421 ccagcgcctg ggccgtggtc tgcggcagcc ggtagaccgt cgtgatgacg ggcacggcca
     3481 gcaccgcgcc gcccacgctg aaaaacccgc ccagcgtgcc ggtggccacg ctcaatgccg
     3541 ccgccctgcg gggagtgaag gccggcgccg accgcgcgga cgccgcagcc gccgcgcagc
     3601 ggcgcttgcg gcgggcctgc accacgtaga acagcgcgac gcagaacagg aagcccgcga
     3661 agctcaggcg cagcagccgg ggatcgatgc ccagcgccag gcgggctccc acccaggtaa
     3721 agaaaacgga aaccagcgcg ccggcgaaca ccacccgcag gtcgaactgc gcatgctggt
     3781 agtacttgcg caccgatagc atcacggtcg gcagcaccag gaccagcgcc gtgccctgcg
     3841 ccagctgctg cggcatgccc aggaccaggg cgagcgccgg gatggcgatc aggccgccgc
     3901 cgatgcccag caggccgccc aggaaaccga tcatcgcccc ggcgaccagg cagatgccgg
     3961 ccaccgccag caagctcacc gcagcttctc cagggcggcg tccaggcggt ccaccgccca
     4021 gatctccagc ccttctatcg gctgcttggg cgcgttggcc ttggggatca gcgccgtgga
     4081 gaagcccagc ttggccgcct cgcgcaggcg ctcctggccg cgcggcgccg ggcggatctc
     4141 gccggccaag ccgacctcgc cgaaagccac cagcccgcgc ggcaggggct tgtcgcgcag
     4201 cgaggacatg atggccagca gcacgggcaa atcggccgcc ggctcggtga tgcgcacccc
     4261 gcccacggca ttgacgaaga cgtcctggtc gtaggtgctc acgccggcat ggcgatgcag
     4321 caccgccagc agcatggcca ggcgattgcc ttccaggccc acggtgagac ggcgcggatt
     4381 gggcgcgtgc gaggcgtcga ccagggcctg cacctcgacc agcaggggcc gggtgccctc
     4441 ctgcgtggcc atgacgcagg agccggcgac ctgctgcggg tgctgcgaca ggaacagggc
     4501 cgaggggttg gccacgccgc gcagcccccg gtcggtcatg gcgaacacgc ccagctcgtt
     4561 gaccgcgccg aagcggttct tgaaggcccg caccaggcgg aacgaagaat gcgtgtcgcc
     4621 ctcgaaatac agcacggtgt cgacgatgtg ctcgagcacg cgcggaccgg ccagcgcgcc
     4681 gtccttggtg acgtggccga tcatcacgat ggtgatgccg gtctgcttgg ccaggcgcgt
     4741 gagctgggcc gcgcactcgc gcacctgcga caccgagccc ggcgcggcgg tgagttcgcc
     4801 gctgtagagc gtctggatgg agtcgatcac ggcaacggaa ggcttctgct cggccacggc
     4861 ggcctggatg gcctcgagcc ggatctcggc gagcaggtcg accttgcccg tcgtgagccc
     4921 cagacggcgg gcgcgcaatg ccacctgctc ggccgactcc tcgcccgtga cgtacagcac
     4981 gggcacggcc tcggacaggg acgccaaggc ctgcagcagc aaggtggact tgccgatgcc
     5041 cgggtcgccg ccgatcagca ccaccgcgcc gggcaccagg ccgccgccca ggacgcggtc
     5101 gaattcttcc agcccggtgg gctggcgcgg ggtttcgcgc gcttcgatgt cggccaggct
     5161 gcgcaccggc gtcgaggccg ccagcggggc atagcggtgc gccgccgagg cggatgactg
     5221 cggcgtctct acggtttctt caagcgcgtt ccaggcgccg caatgcgggc acttgccctg
     5281 ccatttgagg ctggtgccgc cgcactcgga gcagacgtag atcgatcggg atttagccac
     5341 ggcaagatgc cacccgagcg agccgccggg ccgccccaag gcgagcgacg cgagtacccc
     5401 cttggggggt cggcgaacac gcagtgtgag ccggggggct caccgtctcc ctcccagcgc
     5461 cccgccgccg tccacgccca gcacctggcc ggtgatgtag ccggcctcgt cggacaggag
     5521 gaaggcagcg gcggcggcca cgtcggccgg cgtgccgaag cgcttcatgg ggatggagga
     5581 tagcgacagt ttttcggcct cgctgcccgc gggatggttc tggcggaaca gctcggtttc
     5641 gatggggccc ggcgcgatgg cgttggcggt gatgccgtac tcggccagct cgagggccca
     5701 ggtgttggtg cagcccacca gcgcgctctt ggccgccgaa taggcggtgc gatcgtagcc
     5761 gccgtggatg gcgcggctgg cgatgttgac gatgcggccg gcacggcgga ccttcatcga
     5821 ctcgacgaag gtttgcgtga cctgcacggc cacgcggaca ttgagatcga gcacgcggta
     5881 gagcgtggcc aggtcgatgg aacccagcgg ctgcggcgac accacgccga cgttgttgac
     5941 caccgcatcg acggggtact tttcgcggat ctcgcgcagc acttcttcgg tgcggcccgc
     6001 gtcggccagg tcgcaggcat aaagatagcc gggaaaatcc acgtcttcgg tgttgcgggc
     6061 aatgcccacc acatggcagc cctggtcggc caggcgctgc gtcagtgccc agcctatgcc
     6121 cttggtggcg cctgtgatca acacgcattt gtctttcatg gatcgaaccc cttgtagcgt
     6181 tgtattcagc tcagcgtctg caccggcacc cgcagggcca gcgcgcacat cagctcgtag
     6241 ccgatggtgc ccgaggcttg cgccacctcg tcgaccgacg ggccgccctc gccccagagc
     6301 acgaccggcg cgccgatgcc ggcatccggg accggatcga gatcgaccgc gaccatgtcc
     6361 atggacaccc ggccgaccac ccgcgtgggc atgccggcga cggtgatggg cgtgcc
//
LOCUS       DASWPO010000232         4021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2375665, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000232 DASWPO010000000
VERSION     DASWPO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375665"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VGC69_12295"
     CDS             complement(<1..1109)
                     /locus_tag="VGC69_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0296103.1"
                     /translation="MHSWLQRFLSFSPGPGIVPKLVVACIGLAACGVLAAGLAVALAW
                     PSLPDLHAMTDYRPNVPLRVYTADKVLIGEFGEEHRNVLRFDEIPQLMKDSVLAAEDD
                     KFYQHGGIEWTGVLRAGFTNLIHMSKVQGASTITMQVARNFYLSSEKTYARKFYELLL
                     TLKIESQLSKDQILELYMNQIYLGHRSYGFAAAARTYFGKPLADVTPAEAAMLAGIPK
                     APSRANPMTDLNRARERQHYVLGRMRALGKLTDEQYQAALAQAITVRGSGSDGGPSRG
                     YAVHGEYPAELARQLMYGVYQENAYTQGLDVYTTIDSKSQQTAYQAVRDGVLDYTRRS
                     PYPGPEDQIDLPPGIENNPQALDEVLDSVQDNTPD"
     gene            complement(1186..2013)
                     /locus_tag="VGC69_12300"
     CDS             complement(1186..2013)
                     /locus_tag="VGC69_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0296104.1"
                     /translation="MTASPPASSPWRSINVDDYQHVPRRVTAMAKEFSDGSNTGPHTH
                     ARGQLIYAVAGSMRVSTAQGVWALPSRRALWVPPGIEHAVAMLGDVSMRTLYLNADAA
                     STLGRDCCVIEVSDLLRALILSLVAEPIEYALEGRSGQIAALILTELSLAARVPLHIP
                     WPQDRRLQAVCQAILKQPGTHRTVHEWGHEVGASGRTLIRLFQSELGLNYREWVQQVR
                     LADALGRLSMGQPIARIAAELGYRSPSAFAAMFRRVLGTSPNHYLNQAASGVAPGKA"
     gene            2118..3359
                     /locus_tag="VGC69_12305"
     CDS             2118..3359
                     /locus_tag="VGC69_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296105.1"
                     /translation="MSTVLQTTAPSVAPEQPAANSTAFKVLGTISAAHMANDMIQSIL
                     LAIYPMLKANFSLSFAQIGLLTLVYQFAASLLQPWIGLYTDKKPMPYSLPVGMGFTLL
                     GLLMLSVAPSYAFLLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSLFQVGGNLG
                     SALGPLLAALVIIPHGQRSVAWFSLVALVGIVLLTQIGRWYSANRWRLKPKARKAGGA
                     AELSRARVGMTLAVLGMLVFSKYFYLSSLNSYFTFYLIDKFGLPVRSAQLFLFLFLAS
                     VAVGTLIGGPVGDKIGRKAVIWTSILGVAPFTLLLPHANLFWTGVLVMVIGLVLASAF
                     SAILVYAQELVPGKVGLVAGLFFGFAFGMGGVGAALLGRLADATSIEFVYQVCAFLPL
                     LGLLTVFLPDIEGKKAKVAVA"
BASE COUNT          587 a         1199 c         1461 g          774 t
ORIGIN      
        1 ctgtcggggg tgttgtcctg cacgctgtcg agcacttcat cgagcgcttg cggattgttc
       61 tcgatgccgg gcggcaggtc gatctggtct tccgggccgg ggtagggcga gcggcgggtg
      121 tagtcgagca cgccgtcgcg cacggcctgg taggcggtct gctggctctt ggagtcgatg
      181 gtggtgtaga cgtcgaggcc ctgggtgtag gcgttttcct ggtagacgcc gtacatcagc
      241 tggcgcgcga gctcggccgg gtattcgcca tgcacggcgt agccgcgcga cgggccgccg
      301 tcgctgccgc tgccgcgcac ggtgatggcc tgggccagcg cggcctggta ttgctcgtcg
      361 gtgagcttgc ccagcgcccg catgcggccc agcacgtagt gctggcgctc gcgggcccga
      421 ttcaggtcgg tcatggggtt ggcgcgcgaa ggcgccttgg ggatgcccgc cagcatggcg
      481 gcctcggccg gcgtgacgtc ggccagcggc ttgccgaagt aggtgcgagc cgccgccgcg
      541 aagccgtagc tgcgatggcc caggtagatc tggttcatgt acagctcgag gatctggtcc
      601 ttgctgagct gcgattcgat cttgagcgtg agcagcagtt cgtagaactt gcgcgcgtag
      661 gttttctcgg acgagagata gaagttgcgc gccacctgca tggtgatggt gctggcgccc
      721 tggaccttgg acatgtggat caggttggtg aagccggcgc gcagcacgcc ggtccactcg
      781 atgccgccat gctggtagaa cttgtcgtcc tcggcagcca gcaccgagtc cttcatgagc
      841 tgggggatct cgtcaaagcg cagcacgttg cggtgctctt cgccgaattc gccgatgagc
      901 accttgtcgg ccgtatacac gcgcagcggc acattgggcc ggtagtcggt catggcgtgc
      961 aggtcgggca ggctgggcca ggccagggcg accgccaggc ccgcggccag gacgccgcag
     1021 gcggcgaggc cgatgcaggc aacgacaagc ttggggacaa tgccggggcc gggggaaaag
     1081 gacagaaatc gttggagcca gctatgcatg gcccattatc gcaggtgatg ggccggcgcc
     1141 cgaatcgggg ccgcgcgctg gaaacgggtt ggaacaagtt ggggtttagg ccttgccggg
     1201 cgccacgcct gaagcagctt gattcagata atggttgggc gatgtgccca ggacgcgacg
     1261 gaacatggcc gcgaaggcgc tggggctgcg gtagcccagc tcggcggcga tgcgggcgat
     1321 gggctggccc atggacaatc ggcccagggc atcggccagc cgcacctgct gcacccattc
     1381 gcggtagttc agccccagct cggactggaa gagccggatc agggtgcgcc cgctggcgcc
     1441 gacctcatgc ccccattcgt gcacggtgcg gtgcgtgccg ggctgcttga ggatggcctg
     1501 gcagacggcc tgcaggcggc ggtcctgcgg ccagggaatg tgcaggggga cgcgcgcggc
     1561 cagcgacagt tcggtgagga tcagggcggc gatctggccg ctgcggcctt cgagcgcgta
     1621 ttcgatgggc tcggcgacca ggctgaggat gagtgcgcgc agcaggtccg agacttcgat
     1681 cacgcagcag tcgcggccca gggtcgaggc ggcgtcggcg ttcaggtaca gggtgcgcat
     1741 ggacacatcc cccagcatgg ccacggcatg ctcgatgccg ggcggcaccc acagggcgcg
     1801 ccgcgagggc aacgcccaga cgccttgcgc ggtgctcacg cgcatgctgc ccgcgacggc
     1861 gtagatcagc tggccgcggg cgtgggtgtg cggcccggtg ttcgagccgt cgctgaactc
     1921 cttggccatg gcggtgacgc gccgcggcac gtgctggtag tcgtcgacgt tgatgctgcg
     1981 ccagggggag gaggcgggcg gagaggcagt catgccgcta gtgtcacttt ttcgatgaaa
     2041 attgtcactt accctatttt aagcctggct acactatggc caggagacac tacaacccaa
     2101 aatcccgagc cacgacgatg agtaccgtat tacagaccac cgccccgagc gtcgcgcccg
     2161 agcagcccgc cgccaattcc accgccttca aggtgctggg caccatcagc gcggcgcata
     2221 tggccaacga catgatccag tcgatcctgt tggccatcta tcccatgctc aaggccaatt
     2281 tctcgctgag cttcgcgcag atcggcctgc tgacgctggt ctaccagttc gcggcgtcgc
     2341 tgctgcagcc gtggatcggc ctttataccg acaagaagcc gatgccttat tccctgccgg
     2401 tgggcatggg cttcaccttg ctgggcctgc tgatgctgtc ggtggcgcct tcgtacgcct
     2461 tcctgctgct ggctgcggcg ctggtgggca cgggctcgtc ggtgttccat cccgagtcgt
     2521 cgcgcgtggc gcgcatggcg tcgggcggcc ggcatggcct ggcgcagtcg ctgttccagg
     2581 tgggcggcaa tctggggtcg gcgctggggc cgctcctggc ggcgctggtg atcattccgc
     2641 acgggcagcg cagcgtggcc tggttttcgc tggtggcgct ggtgggcatc gtgctgctga
     2701 cgcagatcgg ccgctggtat agcgcgaacc gctggcgcct caagcccaag gcccgcaagg
     2761 ccggcggcgc ggccgagctg tcgcgcgccc gggtggggat gacgctggcg gtgctgggca
     2821 tgctggtgtt ctccaagtat ttctacctgt ccagcctcaa cagctatttc acgttctacc
     2881 tgatcgacaa gttcggactg ccggtgcggt cggcgcagtt gttcctgttc ttgttcctgg
     2941 cgtcggtggc ggtggggacg ctgatcggcg ggccggtggg cgacaagatc ggccgcaagg
     3001 cggtgatctg gacctcgatc ctgggcgtgg cgccgttcac gctgctgctg ccgcacgcga
     3061 atctgttctg gacgggcgtg ctggtgatgg tcatcggcct ggtgctggcg tcggcgttct
     3121 cggcgatcct ggtgtatgcg caggagctgg tgccggggaa ggtgggattg gtcgcggggc
     3181 tgttcttcgg ctttgccttc ggaatgggag gcgtgggggc cgcgctgctg ggcaggttgg
     3241 cggatgcgac gagcatcgag tttgtttatc aggtttgcgc gttcctgccc ttgctggggt
     3301 tactgacggt gttcttgccg gatatcgaag ggaagaaggc caaggttgcg gttgcttgag
     3361 ttggttcgtt cgtttttgat gtgtctgttc gtggttcttg aggcgtccgt cgcggggtgg
     3421 gaagcccttg ggcgcttcac tgagccggtc gggcgcccag cgcgcccgac accgcatcgg
     3481 cacgctcgtc cgcgcctgcg cgctcccttc gcgtccgcct cgctcggctc agaggcgcgc
     3541 ccaagggctt cccaccccgc gacggactca tggatcggaa tgatcaaggc cgtcggggcg
     3601 agtggggttc ttgcgctttg tggtcccggc tggggtgggt ggggagatct tgacgcgctg
     3661 gttaaatggg gctgcccagc gcagccccat ttaacgtcgg gtctgggcat gcgtagggtg
     3721 ttgtgaggcg tggattggtg tgtatgcccg ccgccattgc atacaacggg tgctgcaagg
     3781 gcatcagcca aacctgtgtc agcggtcgtt gtctgctacg gtggcgagtc agacactggc
     3841 ctatgccata ccatcaaccg ctgcatgcaa cggtccgaga caaaccgggc tgcgcagggc
     3901 agcccggttt ggcgagcgaa ccaagatttt cctcccaccc ccagccttgg cccacgaagc
     3961 gccaagcacc caacacgtcc ccagcaatgc tgggaacaca aactactgca gtcagtcggg
     4021 g
//
LOCUS       DASWPO010000233         7214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1841663, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000233 DASWPO010000000
VERSION     DASWPO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841663"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGC69_12310"
     CDS             <1..210
                     /locus_tag="VGC69_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296106.1"
                     /translation="RRNQAATAGWKSASASALACGAVMPTYIGAAASKSIGLRCIAHT
                     FRTPRWRSAGRGHGTGDAQRGGMRI"
     gene            complement(207..632)
                     /locus_tag="VGC69_12315"
     CDS             complement(207..632)
                     /locus_tag="VGC69_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0296107.1"
                     /translation="MKNILIPVDGSANSLRAVHYVIDYVREHGPCTVHLLNVQPLMAS
                     GIVPTFIGQETIQEYYEVEAGAALAEAKAAMDKAGVTYLAAHRIGSVADSIAAYATEH
                     QCDHIVMGSRGLGAVESLLLGSVTLKVLHLIHIPAVLIN"
     gene            complement(867..1526)
                     /locus_tag="VGC69_12320"
     CDS             complement(867..1526)
                     /locus_tag="VGC69_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY0296108.1"
                     /translation="MPVFLSALVRRVDPSLLVRIVARGLAAGCVAGGLAMLGGCAAPR
                     ISAHVTSFQQWPADAAGQAYAFAPVPSEQASNLEFQAYRDVVRAGLGSIGVVEAKPGQ
                     VARFTVDYQYGVQPVQTIVRQPYDPDFDGGFYRSRWWGRPYWGPDWVDVPVVRYRNWL
                     NLQIRDASRGGAEVYRSLAFTLSDRDGLLHSMPYLVRALFDRFPGRNGEEHEVEYPLP
                     Q"
     gene            1670..2173
                     /locus_tag="VGC69_12325"
     CDS             1670..2173
                     /locus_tag="VGC69_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0296109.1"
                     /translation="MSRRRPSEHGWLAYYRELIGTWAKGNSLRHDAEDAAHDAVANML
                     GQKQAAVLDQKAYLYRASRNRLINEVRRQTRREMLSLEDLADGDHPLADPDALLHTRE
                     LVQALERALERLPLKKRQAYIYHRLEGYTQAEIAGKMGVALNTVERYIMDATREIREQ
                     LQNFCPK"
     gene            2298..3284
                     /locus_tag="VGC69_12330"
     CDS             2298..3284
                     /locus_tag="VGC69_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY0296110.1"
                     /translation="MTASIDPDTLQDPRDAAAYWFARERSGHMDEAERQDFENWRCAD
                     PEHDLEYRRAKGIWHATLLIPQERLRTLAREPQRTGAALYLPRRRLVFGLGAACAAAV
                     AAAVALPELDSPTYSDRFATGHGERRQVALPDDSLIDLNTDTSLAVHFYAGRRVVELA
                     AGEAAFSVARNADRPFSVHAGDTVVRVTGTRFDVRRDGKEVLVAVESGSVEVKRGPWW
                     RRSTAQLGAGQTAQTRTQGLATTSQADVESLLAWRHGRLVFQDTPLSQAIAEINRYAP
                     HAIQLNDAALARVRIAGVFSTDDTQPFLDLLPTIAPVRVLRLPDGTPVVARR"
     gene            3529..5964
                     /locus_tag="VGC69_12335"
     CDS             3529..5964
                     /locus_tag="VGC69_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0296111.1"
                     /translation="MDRRIPHSTARRRALPHSPAFSNGLTALAVSLLLAFGLPSPSAQ
                     AQEAPVQVSIAAQPLGSALVLLANQYALELAYSPATVAGLNAPAVSGQLTADQALQQL
                     LAGTGIEFKRSGKNVSLSKPGQSPVEQLPAISVVGNALERAWGPVDGYAAQRSASATK
                     TDTPLLETPQSISVISRAQVQAQNVQNLVQAAQYTPGVQTSIDPVDNRFDSLRIRGFA
                     PTIFLDGMPLPSGSTGYGEPKVDPYILERIEILRGPSSALYGAVPPGGLVNLVSLRPP
                     ETPVHSVQLQANTYGRAETAFDFGGPVDDDGKLSYRLTGLGYGGGTQIDHTKESRALI
                     APSLTLRPDADTTLTLLGEYQRDNSGVQIQFLPAYGTLLNNPNGTVPYGKNVGEPDYD
                     HYSRTQAWAGYDFEHRFNDTTKVRQKLRYVDIDTDVRAIIGAGLQPDMRTLNRQNFHV
                     PEATQSLMMDNDLEFNFKTGQVAHTALLGLDYHWASSDTQQGFGSAPAIDLFNTRYGA
                     PVATPASSSSSSQNQNQLGLYLQDQLAYEHWRLTLSGRHDWVDTRTTNRIANTSQSQE
                     EGAFSGRAGLNYVFDSGISPYVAYSRSFQPVIGTAFDSTPFNATSGQQYEAGVKYQPP
                     GRDISMTAAAFTLTQENALTVDTEHPLFQTQAGQVRSRGIELQAIGKITPDVNLVAAY
                     TYTNASVTKSNGTDLGKQVIATPRHQASLWVDYTVPGGALRTLSLGAGMHYFGRSYGN
                     AANTIGIPSNTLVDLAISYDLGALNARLKGARVSLAVNNLFDRRYVATCASLSQCYYG
                     TSRVAMATLRYDW"
     gene            5983..7074
                     /locus_tag="VGC69_12340"
     CDS             5983..7074
                     /locus_tag="VGC69_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0296112.1"
                     /translation="MPWGSFKAWYGVHRWTSLACTLSLLLLCLTGAPLVLKEEIGVWS
                     GSTVEPPDMPGVTTRVSIDGLIADARRRRPGDEVRYVSQSDDAPAWFVAMGRTADAAE
                     ATAVYKYDARTGAMIHDVTQGGKVMRAIRSLHVDLFAGLPGTLFIGLMGLMFVASTLS
                     GLMVYRFHWQAVPFGTIRRNKSARLFWLDWHNLLGMMTLAWLILVGVTGVINTLALPL
                     LGYWQAHELPAMTQAWRGPAAAIDSGSAQRAMEAARAAMPGMEVDFLGMPGSRFATPR
                     HYMVFMHGDTFWTSRILQPVMVQADNAEVVSTRALPWYLVALLVCQPLHFGDFGGMPL
                     KILWICLDLGTIGLLLSGLYLWRRRKAKA"
BASE COUNT         1311 a         2459 c         2223 g         1221 t
ORIGIN      
        1 cggcggaacc aggcggcgac ggcgggatgg aaatcggcca gcgcgtcggc gctggcctgc
       61 ggggcggtca tgcctactta tattggggcg gccgcgtcga agtcaatcgg gttgcgctgt
      121 attgcgcata cgttcaggac gccgcgatgg cgctcggcag gccgcggcca tggcaccggc
      181 gatgcgcaac gtggcggcat gcgcatctag ttgatcaaaa cggccggaat atggatgaga
      241 tgcaggactt tcagggttac cgatcccagc agcaggcttt ccacggcccc caagccacgc
      301 gatcccatga cgatgtgatc gcattgatgc tctgtggcgt aggctgcgat cgagtcggcc
      361 acgctgccga tgcgatgggc cgccaggtac gtgactcccg ccttgtccat ggccgctttc
      421 gcctcggcga gggccgcgcc ggcctcgact tcgtaatact cctggatcgt ttcctggccg
      481 atgaaggtcg gcacgattcc tgaagccata agaggctgga cgttcagcag atgaacagtg
      541 caggggccat gttctcgaac atagtcgatc acgtagtgca cagcgcgcag ggagttggcc
      601 gagccatcta cggggataag tatgtttttc acgttgtctc caggaaatcg gacgccttcg
      661 gcaaaatagc cgttcggcgg gcatatcgcc aattatggcg acgtcaccat cgttttaatt
      721 gatgccgatc aatcccggct cttttttttg gatgcccctg accaggaaga caagacctca
      781 ccgtccccca atccgattta tcttacttta cataatatac attatgcgca tttcaagccg
      841 acttgttcga acccagcgga tcggccttat tgaggcagcg gatattcaac ctcgtgctct
      901 tcgccattgc gtccgggaaa acggtcgaac agcgcccgca ccagatacgg catgctgtgc
      961 agcagcccgt cccggtcgct cagggtaaag gccaaggacc ggtacacctc ggccccgcca
     1021 cgggaagcat cgcggatctg caggttcagc caattgcgat agcgcaccac tggcacgtcc
     1081 acccaatccg gcccccagta aggcctgccc caccagcgcg accggtagaa cccgccatcg
     1141 aaatcgggat cgtagggctg gcgcacgatg gtctgcaccg gctggacgcc gtattggtaa
     1201 tcgacggtga aacgcgccac ctggcccggc tttgcctcga ccaccccgat cgagcccagg
     1261 cccgcccgca cgacgtcgcg gtacgcctgg aactcgaggt tgctggcctg ctcggacggc
     1321 accggcgcga aggcataagc ctggcccgcg gcatcggcgg gccattgctg gaacgaggtc
     1381 acgtgcgccg atatcctcgg cgcggcgcag ccacccagca tggcgagccc gcccgcgaca
     1441 cagcccgcgg ccaggccgcg cgcaacaatg cggaccagca ggctgggatc gactcgccgc
     1501 accagggcag ataagaaaac cggcatagac ctcggcgcct ggggcgcccc ttctgcaaaa
     1561 cttatgggat tatttcaata cctgcatgag atagtaatga gaattattgt taacatcaaa
     1621 ccctaacttg ttgcactgaa attttctccc cgcttcgtcc cacgccgcca tgtcccgccg
     1681 ccgcccttcc gaacacggct ggctcgccta ttatcgcgag ctgatcggca catgggccaa
     1741 aggcaactcg ctgcgccatg atgccgagga cgcggcgcac gacgccgtgg ccaatatgct
     1801 cggccagaag caggcggcgg tgctcgacca gaaggcctac ctgtaccggg cctcgcgcaa
     1861 ccggctgatc aacgaagtcc ggcggcagac ccgccgcgaa atgctgtccc tggaagacct
     1921 ggcggacggg gatcatcccc tggccgaccc ggacgccctc ttgcacactc gcgaactggt
     1981 gcaggcgctg gaacgcgccc tggaacgcct gcccctgaag aagcgccaag cctatatcta
     2041 ccatcgcctg gaaggatata cccaagccga gatcgctggg aaaatgggcg tcgcgctcaa
     2101 cacggtcgag cggtacatca tggacgccac ccgggaaatt cgtgaacagt tacaaaactt
     2161 ctgcccgaaa tgatcaggga tccaggccgc tgcggtgtct ttatgatgag aaggcgtgaa
     2221 ttggcctggc ctggacatgc gggccatgcc gaagcccgcc cttgccccct atgccagcct
     2281 gcgctggcga ctgcctgatg actgcttcca tcgacccgga tacgctgcaa gaccctcgcg
     2341 acgcggccgc ctattggttc gcccgggagc gctcggggca catggacgag gccgaacgcc
     2401 aggacttcga gaactggcgc tgcgccgacc ccgagcatga tctcgaatac cggagggcca
     2461 agggcatctg gcatgccacc ctgctgatcc cgcaggagcg gctgcgcacg ctggcacgcg
     2521 aaccccagcg gacgggcgca gcgctgtact tgccgcgccg ccggctggtg tttggcctgg
     2581 gcgcggcgtg cgccgccgcc gtggcggcag cggtggccct gcccgagctg gactcgccca
     2641 cctactcgga ccggttcgcc accggacatg gcgaacggcg ccaggttgcg ctgccggacg
     2701 actccctcat cgacctgaac acggacacct cgctggcggt gcacttctac gccgggcggc
     2761 gcgtcgtcga actggccgcg ggcgaagccg ctttcagcgt ggcccgcaac gcggaccgcc
     2821 ccttctctgt gcacgcgggc gacaccgtcg tgcgcgtcac ggggacgcgc ttcgacgtgc
     2881 ggcgcgatgg caaggaggta ttggttgccg tcgaatccgg ctctgtcgaa gtcaagcgcg
     2941 gtccctggtg gcgccgcagc accgcccagc tcggcgccgg acagacggcc cagacccgca
     3001 cccagggact tgccacgacg agccaggcgg atgtcgagtc gctgttggct tggcggcacg
     3061 ggcggctggt cttccaggac acgccgctgt cccaggccat cgccgagatc aaccgctacg
     3121 cgccccatgc catccagctg aacgacgccg cgctggcccg ggtgcgcatc gccggggtct
     3181 tcagcaccga cgacactcag cccttcctcg acctgctgcc gaccatcgcg ccggtgcgag
     3241 tgctgcgcct gcccgacgga acacccgtcg tcgcccggcg ctaggcagac ctctgatttg
     3301 cgtggacgca aggcacctct gggtgtcttt ttgcgctgcg gccgccatcc caaatgaaaa
     3361 tacatcttgt ttcaaacaaa tcggcagatt tcatcaggta ttgggagagc tgcggtgtct
     3421 gttttatgag gaaggctgct tttgccaggc aagacggcct ccgcagaacc tttggcctaa
     3481 gggtggtggc gcgtgagcgc cttggtgaat tacctggaaa actgtcccgt ggatcgtcgc
     3541 attccccaca gcacggcacg ccgtcgcgcg ctgccgcact ctcccgcgtt ctcgaacggc
     3601 ctgaccgccc tggcggtgtc gctactgctg gcattcggtc tgccttcgcc gtccgcccag
     3661 gcccaggaag cccccgtgca ggtcagcatc gccgcgcagc cgctgggcag cgcgctggtg
     3721 ctgctcgcca accaatacgc gctggagctg gcctactccc cggccaccgt ggcggggctg
     3781 aacgcgcccg ccgtgtcggg ccagctgacg gcagaccagg cattgcagca gttgctggca
     3841 ggcacgggca tcgaattcaa gcgcagcggc aagaacgtgt cgctgtcgaa acccgggcaa
     3901 tcgcccgtgg agcaactgcc tgcgatctcc gtggtgggca atgcgctgga acgcgcctgg
     3961 gggccggtcg acggctatgc cgcccagcgc agcgcctcgg cgaccaagac cgacacgccg
     4021 ctgctggaaa cccctcaatc catttccgtg ataagccgcg cccaggtgca ggcgcaaaac
     4081 gtccagaacc tggtgcaagc cgcgcaatac accccgggcg tgcaaacgag catcgacccc
     4141 gtggacaacc gtttcgattc gctgcgcatc cgggggtttg cacccacgat attcctggac
     4201 gggatgccct tgccctcggg ttcgaccggc tatggcgagc ccaaggtcga cccctacatc
     4261 ctcgaacgca tcgagatcct gcgcggcccc tcctccgcgc tgtacggcgc cgtgcctccc
     4321 ggcggcctgg tcaacctcgt cagcctgcgg ccgcccgaga cgccggtgca ctcggtgcag
     4381 ttgcaagcca acacctacgg ccgagcggaa accgccttcg atttcggcgg cccggtggac
     4441 gacgacggca aactgtccta ccggctgacc ggactgggct atggcggcgg cacgcaaatc
     4501 gaccacacca aagaaagccg cgcgctgatc gccccgtcgc ttaccctgcg ccccgacgcc
     4561 gacaccaccc tgaccctgct gggcgaatac cagcgcgaca acagcggcgt gcagatccag
     4621 ttcctgccgg cctacggaac cctgctgaac aatcccaacg gcacagtccc ttacggcaag
     4681 aacgtcggcg agccggatta cgaccattac tcgcgcaccc aggcctgggc tggctacgat
     4741 ttcgagcatc ggttcaacga tacgaccaag gttcgccaga agcttcgcta tgtcgacata
     4801 gacaccgacg tccgcgcgat catcggcgcc ggcctgcagc cggacatgcg cacgctcaac
     4861 cgccagaact tccacgtgcc cgaagccacc cagtccttga tgatggacaa cgatctcgag
     4921 ttcaacttca agaccggcca ggtagcgcat acggcgctgc tggggctgga ctatcactgg
     4981 gcctccagcg atacccagca agggttcggc agcgcgcctg ccatcgatct tttcaacacg
     5041 cgctacggcg ccccggtcgc cacgccggcg agttcatcca gcagttcgca gaaccagaac
     5101 cagctgggcc tctacctgca ggaccaactg gcctatgagc actggcgcct gacgctcagc
     5161 ggccgccatg actgggtcga cacccgcacg accaaccgca tcgccaacac ctcccagagc
     5221 caggaagaag gcgccttctc ggggcgcgcc ggcctcaact acgtgttcga ttccggcatc
     5281 tcgccttatg tcgcctactc gcgctctttc cagcccgtca tcggcaccgc cttcgacagc
     5341 acgccgttca atgccacgag cggccagcag tacgaagccg gcgtgaagta ccagccgcct
     5401 ggccgggaca tttcgatgac cgccgcggcc ttcacgctga cgcaagagaa tgccttgacg
     5461 gtcgataccg agcatccgct attccagacg caggcgggcc aggtccggtc gcgcggcatc
     5521 gagctgcagg cgatcggcaa gataacgccc gatgtcaacc tggtggcagc ctatacctat
     5581 accaatgcca gcgtcacgaa aagcaatggc accgacctgg gcaagcaggt catcgccacg
     5641 ccgcggcacc aggcctcgct gtgggtcgac tacacggttc cgggcggcgc cctgcgcacg
     5701 ctcagcctcg gcgccggcat gcattatttt ggccgcagct acggcaacgc cgccaatacc
     5761 atcggcatcc cgtccaacac gctggtcgac ctggcgataa gctacgacct gggcgcgttg
     5821 aatgcccgat tgaagggggc ccgcgtctcg ctggcggtga acaacctgtt cgacaggcgc
     5881 tacgtcgcga cctgcgccag cctgagccag tgctattacg gcaccagccg cgtggccatg
     5941 gcgacgctgc gctacgactg gtaggcgccg atgcgctggc gcatgccatg gggaagtttc
     6001 aaggcctggt acggcgtcca tcgctggacc agcctggcct gcaccctctc tttgctgctg
     6061 ctctgcctga cgggcgcgcc cctcgtgctc aaggaggaga tcggcgtctg gtccggcagc
     6121 acggtggagc cgcccgacat gccaggcgtg accacgcggg taagcatcga tggcctgatc
     6181 gccgatgcgc ggcgacggcg gccgggcgac gaagtccgct atgtctcgca aagcgacgac
     6241 gcgcccgcgt ggttcgtggc catgggccgc accgcggacg ccgccgaggc cacggcggtc
     6301 tacaagtacg atgcccgcac gggcgccatg atccatgatg tcacgcaagg cggcaaggtc
     6361 atgcgcgcga tccgcagcct gcacgtcgac ctgttcgcgg gcttgcccgg cacgctgttc
     6421 atcggcctga tggggctcat gttcgtcgcc tcgaccctgt ccggcttgat ggtgtatcgc
     6481 tttcattggc aagcggtgcc tttcggcacg atccgcagga acaagtccgc gcgcctattc
     6541 tggctggact ggcacaacct gctgggcatg atgaccctgg catggctgat tctggtgggc
     6601 gtcacgggcg tcatcaatac gctggccttg ccgctgctgg gctattggca agcgcatgag
     6661 ctgcccgcca tgacgcaagc atggcgaggg ccggccgcgg ccatcgacag cggctcggcg
     6721 caacgagcga tggaggcggc gcgggccgcc atgccaggca tggaagtcga tttcctcggc
     6781 atgcccggca gccgtttcgc cacgccgcgc cattacatgg ttttcatgca cggcgacacg
     6841 ttctggacct cgcgcatact gcaaccggtg atggtgcaag ccgacaatgc agaagtcgtc
     6901 tcgaccaggg cgctgccctg gtacctggtg gcgctactcg tctgccagcc gctgcatttc
     6961 ggcgatttcg gcggcatgcc gctcaagatc ctgtggatct gcctggacct gggaaccatc
     7021 gggctgctgc ttagcggcct gtacctatgg cgcaggcgca aggccaaggc ttgacctgcc
     7081 ctgcctaggg ctgcccttcg aactcctggg ccccggcgaa agacggcatc cacgggaatc
     7141 tgcgctgggt ccagatctgc cgcgcgggcg tgcccagttc gtagcgctgc ttcaatgcgc
     7201 ccacgcgcag cgaa
//
LOCUS       DASWPO010000234         7334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_591891, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000234 DASWPO010000000
VERSION     DASWPO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591891"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1320)
                     /locus_tag="VGC69_12345"
     CDS             complement(4..1320)
                     /locus_tag="VGC69_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296113.1"
                     /translation="MSDKATRIDLFSLRTVPMRIFHLTWLAFFVCFFAWFSAAPLMPV
                     ISKEFGLTAGQVADINIAAVAATILVRLIVGPLCDRYGPRRVYTWLMILGAIPVFALP
                     FAYDYTTVLLCRLGIGAIGASFVITQYHTSVMFAPNVVGTANAASAGWGNAGGGVAQA
                     VMPMLFAAALWFGLQDSSAWRAALVVPGIAMLLMAAAYWRWGQDCPQGDYLELRRQGV
                     QVEGGKKGGWASFVAAGANYRVWMLFVTYGACFGLELFIHNIAAMYYVNHFHLTLEGA
                     GLAVGVFGLLALFARALGGWVSDKLARRRGLGARPVLLAVMMVGEGLGLLWFSHEESA
                     ALAIVAMAVFGLFTHMACGATYALVPFIDRKALGGVAGIVGAGGNVGAVAAGFVLKGV
                     GDIQQTIIWLGSFVIASAVCALAVRFSQGHAASMQAGVLTADAASR"
     gene            complement(1650..2996)
                     /locus_tag="VGC69_12350"
     CDS             complement(1650..2996)
                     /locus_tag="VGC69_12350"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296114.1"
                     /translation="MEKQATQGLTRHLVEQGLRRSLPGLPDDVRRVGLDCLIDWLGCS
                     FAALDEPAGRIVAEVAREEGGHAQATLLGRRWRGSLQQAALVNGTTSHALDYDDVNLS
                     VPGHMSAAIFPALVALAEYRDVRVADLLVAFAAGYEFACSMGKLVEPAHYANGFHATA
                     TIGCLAAAMACARLLALPVEQACHAVGVAATQAAGLKVMFGSMAKPLHAGLASQSGLR
                     AVLLAGKGFTSRMDALECPQGFVQVHGADFHADQALSAPRGGFHLLNNLFKFHAACYS
                     THSTIEAVVALRREHALAAQSVARIDVVAGEGCSICNLQQPGTALEAKFSLRATAAFA
                     MLGIDTSDLGTWGRVSDPEVRALMERVRVELVPEMGLSEATVAIVQDDGRRLVKSHDC
                     GTPMADKAEQSKRVAAKFRAIAAPALGSGGVGQVLDILGGRSLDGRVAELMSCCRG"
     gene            complement(3005..3964)
                     /locus_tag="VGC69_12355"
     CDS             complement(3005..3964)
                     /locus_tag="VGC69_12355"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296115.1"
                     /translation="MACATAMAVAGFSASAAYPDHPIQLIQPYAAGGPTDIASRVMAQ
                     YLSTQLDQSVVVMPMTGAGGELGSDYASRAKPDGYTLLFAVNSMAIFPFTRSAKNPLP
                     FDPNDFVPIGTVAESAHVIVANPKSGIKSIADLVAAAKKKPDAISYGSAGVGGTTHLP
                     LAFFSNRAGIKLLHVPYKGAAPAMADTMAGVVTLSAPGYSDAVTASVNNGSLVPLAVT
                     SAKRLPFLDKVPTLAEQGYPDMVFPIWYALFAPKGTPQNVVDKLNGALKVMAQDPEYQ
                     KKMYAQGNVATYSTPKELRDLLKTSIEQLGVRLKAAGINLEQE"
     gene            complement(4074..5534)
                     /locus_tag="VGC69_12360"
     CDS             complement(4074..5534)
                     /locus_tag="VGC69_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0296116.1"
                     /translation="MVLHDDKHFDVVVVGCGVAGLSAAVAAADAGAKVAVLERSRYEE
                     RGGNTRYTEAYLRMKSETELSPDFGTLLLDNSGYYIQPDFVEATVRDYENWPALVRAL
                     PFTDPELISFFMDAAPEAIAWLKQHGVTFGEAGFYGLTPRPSPRIAINGGGLQLVETM
                     TACAEKAGVRFFYEMTAFDLVQSDSGAVCGLMATDTGNVPHRFGCKSVILASGGFQGN
                     LEMVVRYIGPKGRYLRPVAQGGYYNRGEGIKMALDIGAAPAGDYAEYHGQPVDPRSSA
                     SEALVMVYPYGVLVNRDGKRFINESPGSIDAHYEETIRLIAEQKKGIAYCILDDKINR
                     INNWKRCVRSDVPPETAPDIKSLGAKLGFDGEAAQRTVDEYNQACTSGTFNPQILDGV
                     STAGLAPPKSNYAVPLDTGPFHAYPIISANTFTLGGLKVNANAQVVKNEGCILPGLYA
                     AGETVGLYYGRYAGATSVLRGAVFGRKAGEHAAGQI"
     gene            5772..7088
                     /locus_tag="VGC69_12365"
     CDS             5772..7088
                     /locus_tag="VGC69_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296117.1"
                     /translation="MEEAYMALGQLVAQANLGAIAAPARQAIDQGIRVSLLCTLFGIE
                     RERWVWPFQERRNAQGRQGPADMLAGLLAPLGGIGYLGTLGGASDLDPMDPGPSHTVL
                     PATLAACTACTLVDATGTPAAGDDDHVGSAVLAGVEAAWRFRSAITGARPGLGFHSPG
                     VFGTLAAAASGARALRLDPQACLNALAIALTRVAGLGINSAASMIGMTHFGWGASHGL
                     EAALLASQGWTASHDFQRGLATLYDADRVAIERLDSAGPLAAAALVFKRYPCNIYLNL
                     LVEMLEEIEEKPVDRIDITLPWVPHLDCPAPRDLRQARNSAQAVAAIAGSGSASYAAF
                     SGPPGPWVPTPEAGRLLPNVRLHMDKAAPTGLRDARVAVQASRGGSVILERERSMREM
                     GGWGIEHARKLMGSPDPHGSIAALYEGSYMQGFAHLAQRRHAAAAA"
     gene            complement(7100..>7334)
                     /locus_tag="VGC69_12370"
     CDS             complement(7100..>7334)
                     /locus_tag="VGC69_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296118.1"
                     /translation="MAYRAAQQGLGVALAQSFLIQEELKNGSLVKIFPPEVASERVYY
                     LMSSPRHCGDPALELFRAWLLDELASGAGPVPA"
BASE COUNT         1281 a         2509 c         2366 g         1178 t
ORIGIN      
        1 cgctcaacgc gacgccgcgt cggccgtcag cacgccggcc tgcatgctgg ccgcatgccc
       61 ctggctgaag cgcaccgcca gcgcgcaaac ggccgaggca atgacgaagc tgcccagcca
      121 gataatggtc tgctggatat cgcccacgcc cttgagcacg aagccggcgg cgaccgcgcc
      181 cacattgccg cccgcgccca cgatgcccgc cacgccaccc agggccttgc ggtcgatgaa
      241 gggcacgagc gcatacgtcg cgccgcaggc catgtgcgtg aacaggccga agacggccat
      301 ggccacgatg gccagcgccg cgctctcttc atgcgagaac cacagcagcc ccagcccttc
      361 gcccaccatc atcacggcca gcagcaccgg gcgcgcgccc agcccgcggc gccgcgccag
      421 cttgtcggac acccagccgc ccagcgcccg cgcaaacagc gccagcaggc cgaagacgcc
      481 cacggccagg cccgcgcctt cgagcgtcag gtggaagtga ttcacgtaat acatcgcggc
      541 gatgttgtgg atgaagagct ccaggccgaa gcacgcgccg tacgtgacga agagcatcca
      601 cacgcggtag ttggcgccgg ccgcgacgaa gctcgcccag ccgcccttct tgccgccctc
      661 gacctgcacg ccctggcggc gcagttcgag gtaatcgccc tgcgggcagt cctgccccca
      721 gcgccagtag gccgcggcca tcagcagcat ggcgatgccc ggcacgacga gcgccgcgcg
      781 ccaggccgac gaatcctgca ggccaaacca cagcgcggcg gcgaagagca tcggcatcac
      841 ggcctgcgcc acgccgccgc ccgcattgcc ccagcccgcg ctggccgcgt tggccgtgcc
      901 caccacattg ggcgcgaaca tcaccgaggt gtggtactgc gtgatcacga agctcgcgcc
      961 gatggcgccg atgcccagcc ggcacagcag caccgtggtg tagtcgtagg caaacggcag
     1021 cgcgaacacg ggaatggcgc ccaggatcat cagccaggta tagacgcggc gcgggccgta
     1081 gcggtcgcac agcgggccga cgatgagccg caccaggatg gtggccgcca ccgcggcgat
     1141 attgatgtcg gccacctgcc cggcggtcag gccgaactcc ttgctgatca ccggcatcag
     1201 cggcgcggcg ctgaaccagg cgaagaagca gacgaagaac gccagccagg tcaggtggaa
     1261 gatgcgcatc ggcacggtgc gcaggctgaa caaatcgatg cgggtcgctt tgtcggacat
     1321 gatctttcct cggcacaaaa tgaaaacggc gccctgcgca tgcggtcgcg aaaccggatg
     1381 cgcagggcgc cgttgccctg ggagcctgtt gccaggccat tcgaaaattc gaaatttgga
     1441 gacccgcggt atggcagcgg atccatccag gaccatgcaa atcttgtgcc aaggtcggcc
     1501 aaacggccgc caagtcgtct atctttttga aatcaaaaag aaaatagccg ctccccgttg
     1561 tcaaaagcgc ggaactgcgg tgtgctgcgg cgcacctatt cagtgccagc aggcacagat
     1621 tccgtgcgtc gaccgctgaa tccgtcctcc tagccccggc agcacgacat caattccgcg
     1681 accctgccgt ccagagaacg cccgcccaat atatcgagca cctgcccgac acctccgctc
     1741 cccaatgcag gcgccgcgat ggcgcggaac ttcgccgcga cccgcttcga ttgctccgcc
     1801 ttgtcggcca tgggagtccc gcagtcatgc gacttgacca ggcgcctgcc atcgtcctgc
     1861 acgatggcga cggtggcctc gctcaatccc atctccggca ccagttcgac ccggacgcgc
     1921 tccatcaaag cccggacctc gggatcgctg accctgcccc aggtgcccag gtcgctggta
     1981 tcgatgccga gcatggcgaa ggccgccgtg gcccggaggc tgaatttcgc ttcgagcgcg
     2041 gtgccgggct gctggagatt gcagatgctg cacccctccc ccgccaccac gtcgatacga
     2101 gcgacggact gcgcggccag ggcatgttcc cggcgcagcg cgacgacggc ttcgatcgtc
     2161 gaatgcgtgc tgtagcacgc ggcgtggaac ttgaacaaat tattcaggag atgaaagccg
     2221 ccgcgcggcg cggacagcgc ctggtcggca tggaaatccg cgccatggac ctggacgaat
     2281 ccctggggac attcaagcgc atccatgcgg ctcgtgaacc ccttgccggc cagcagcacc
     2341 gcccgcagtc ccgactggga cgcaagaccg gcatgcagcg gcttggccat gctgccgaac
     2401 atcaccttca ggccggcggc ctgggtcgcc gccaccccga ccgcatggca ggcctgctcg
     2461 accggcaagg caagcagcct cgcgcaagcc attgccgcgg ccaggcaacc gatggtcgcc
     2521 gtcgcgtgaa agccgttcgc gtaatgcgcc ggctcgacga gcttgcccat gctgcaggca
     2581 aactcgtagc cggcggcaaa agcgacgagg aggtccgcca cccgcacgtc ccggtattcg
     2641 gccagggcga cgagcgccgg gaatatcgcg gcggacatgt ggccgggaac ggacaggttg
     2701 acgtcgtcgt agtccagggc gtgggaggtc gtgccgttga ccagggcggc ctgctgcagg
     2761 ctgcctcgcc atcgccgccc gagcagggtg gcctgggcgt ggccgccctc ttcgcgcgcc
     2821 acctccgcga cgatgcggcc cgcgggctcg tcgagcgccg cgaacgaaca gcccagccaa
     2881 tcgatcaggc agtccaggcc gaccctgcgc acgtcatcgg gaagccccgg caggctcctg
     2941 cgcaagccct gttccacgag atgccgcgtc agtccctgcg tcgcttgctt ttccatggga
     3001 cggcctattc ctgttccagg ttgatgcccg ccgccttcag gcgcacgccc agttgctcga
     3061 tgctcgtctt cagcaagtca cggagttcct tgggcgtcga ataggtcgcg acattgccct
     3121 gggcatacat tttcttctgg tactcggggt cctgcgccat gaccttgagc gcgccgttga
     3181 gcttgtcgac gacgttctgc ggcgttccct tgggcgcgaa cagggcatac cagatcggga
     3241 agaccatgtc ggggtagccc tgctcggcca gggtgggcac cttgtccagg aagggcaggc
     3301 gcttggccga ggtcaccgcc agcgggacca ggctcccatt attgacggag gccgtcacgg
     3361 catcggaata cccgggcgcg gaaagggtca cgacgcccgc catcgtgtcg gccatggcgg
     3421 gcgcggcgcc cttgtagggc acgtgcagca gcttgatccc ggcgcggttc gagaaaaacg
     3481 ccagcggcag gtgggtcgtg ccgcccacgc ccgccgagcc atagctgatc gcatcgggtt
     3541 ttttctttgc cgccgccacc agatcggcga tgctcttgat gccggacttc ggattggcga
     3601 cgatcacatg ggccgattcc gcgacggtgc cgatcggcac gaagtcgttg ggatcgaagg
     3661 gcagcggatt cttggccgag cgcgtgaacg gaaaaatcgc catcgaattc accgcgaaca
     3721 gcagcgtata gccgtcgggc ttggcccggc tcgcatagtc cgaaccgagt tcgccgccgg
     3781 cgcccgtcat gggcatgacc accacgctct ggtccagctg cgtggacagg tattgcgcca
     3841 tcacccgcga tgcgatatcg gtagggccgc ccgccgcgta gggctggatg agctggatgg
     3901 gatggtccgg ataggcggcg gatgcggaaa agccggccac ggccatggcg gtggcgcagg
     3961 ccatgcgctg caatgctcgg tgagcagaaa acgtgatcac aatgttgtct ccacttatag
     4021 gtcgtcagtg agtccatcga gggacccagc gcttttaacg gcaaatcgat tttttatatt
     4081 tggccggcgg catgctcccc ggccttgcgg ccaaacaccg cgccgcgcag cacggacgtc
     4141 gccccggcat accggccgta atacaggccg acggtttctc ccgccgcata gaggcccggc
     4201 aggatgcacc cttcgttctt gacgacctgc gcattcgcgt tgaccttcag cccgcccagc
     4261 gtgaaggtgt tcgcggagat gatggggtag gcatggaacg gccccgtatc caggggcacg
     4321 gcgtaattgg atttcggcgg cgccagcccc gcggtcgaga cgccatccag gatttgcgga
     4381 ttgaacgtgc cgctcgtgca ggcctggttg tattcgtcca ccgtgcgctg ggcggcctcg
     4441 ccgtcgaagc cgagcttggc gcccagcgac ttgatgtccg gcgcggtctc gggcgggaca
     4501 tcgctgcgca cgcagcgttt ccagttattg atgcggttga tcttgtcgtc gaggatgcag
     4561 taggcaatgc cctttttctg ctcggcgatc agccggatcg tctcttcgta atgcgcgtcg
     4621 atggagccgg gcgactcatt gatgaagcgc ttgccgtcgc ggttgaccag gaccccgtac
     4681 ggatacacca tgaccagggc ttccgaggcg ctcgaacgcg gatcgaccgg ctgcccgtgg
     4741 tattcggcgt agtcgccggc gggcgcggcg ccgatatcga gcgccatctt gatgccttcg
     4801 ccccggttgt agtaaccgcc ctgggcgacg ggacgcaggt accggccctt ggggccgata
     4861 tagcggacga ccatctccag gttgccctgg aagccgccgc tggcaagaat gacggacttg
     4921 cagccgaatc gatgcggcac gttgccggtg tcggtggcca tcaggccgca taccgccccg
     4981 ctgtccgact ggaccaggtc gaaggccgtc atttcataga agaaccgcac gccggccttt
     5041 tccgcgcagg cggtcatggt ctccaccaac tgcaggccgc cgccgttgat ggcgatgcgc
     5101 ggcgacggac gaggcgtcag gccgtagaaa ccggcctcgc cgaaggtgac gccatgctgc
     5161 ttgagccacg cgatggcttc cggcgccgcg tccatgaaaa acgagatcag ctccggatcg
     5221 gtgaacggca gggcgcgaac cagcgccggc cagttttcgt aatcgcgcac ggtggcttcg
     5281 acgaaatccg gctggatgta atagccgctg ttgtccagga gcagcgtccc gaaatcgggc
     5341 gacagctccg tctcgctctt catccgcaga taagcctcgg tatagcgggt gttgccgccc
     5401 cgctcctcgt accgggaccg ttcgagaacg gcaaccttgg cgcccgcatc ggcggcggcg
     5461 accgccgcgc tcaagccggc cacgccgcat cccaccacca ccacgtcgaa atgcttgtcg
     5521 tcatgcaaaa ccattgatgt tccttgcgaa tgaggttgtc tacccacctg ctcgatatgc
     5581 ttgatgttct ggactgcccg tttgagcgtc ccgcaagtct tgaggatttc gatctgtctc
     5641 tcaatcgata tttttgtcgc catgcatgag tcgcgcttat gcaaagcacg catgaatatc
     5701 gtgttgtgcc gcgcacaacc ctgtgtatag ttttctacac atgccctgca caaatagaag
     5761 aggagactga aatggaagag gcatacatgg cgctcggcca gctggtggcc caggcgaacc
     5821 tgggcgccat cgcggcgccc gccaggcagg cgatcgacca gggcatccgg gtgtcgctgc
     5881 tgtgcacgct gttcggcatc gaacgcgagc gatgggtatg gcccttccag gaacggcgca
     5941 acgcccaagg ccgccaaggg ccggcggaca tgctggccgg cctgctggcg cccctgggcg
     6001 gcatcggcta cctgggcacg ctgggcgggg cttccgacct cgaccccatg gatcccgggc
     6061 catcccatac cgtgctgccg gccacgctgg ccgcctgcac ggcctgcacc ctggtcgacg
     6121 caaccggcac gcccgccgcc ggcgacgacg atcacgtggg ctcggccgtc ctggcgggag
     6181 tggaagccgc atggcgcttc agaagcgcca tcaccggcgc gcggcccggg ctgggctttc
     6241 acagccccgg cgtcttcggc accctggccg ccgccgcgag cggcgcccgc gcattgcgcc
     6301 tggatccgca ggcatgccta aacgccctgg ccatcgcctt gacgcgcgtg gccggcctgg
     6361 ggatcaacag cgccgcctcg atgataggca tgacgcattt cggctggggc gcatcgcacg
     6421 gactcgaagc ggcgctgctg gcctcgcaag gctggacggc ttcgcatgat ttccagcgcg
     6481 gcctggccac gctttacgac gcagaccgcg tagccatcga acggcttgac tcggcgggcc
     6541 cgctggctgc cgccgccctg gtcttcaagc gctatccctg caatatctac ctcaacctac
     6601 tggtcgagat gctcgaagag atcgaagaaa agccggtgga ccggatcgac atcaccctgc
     6661 cgtgggtgcc gcatctcgac tgccccgccc cccgggactt gcgccaggcg cgcaacagcg
     6721 cgcaagccgt cgccgccatc gcgggcagcg gcagcgcctc gtacgccgcg ttctccggcc
     6781 cgccgggccc ctgggtgccg acgcccgagg cggggcggct gctgccgaat gtgcgcctgc
     6841 acatggacaa ggccgcgccc accggcctgc gcgatgcccg cgtcgccgtg caagcgtcgc
     6901 gcggcggctc ggtcatcctc gagcgtgaaa gatccatgcg ggaaatgggc ggctggggca
     6961 tcgaacatgc ccgcaagctc atgggctcgc cggaccccca cggcagcatt gccgccctgt
     7021 acgagggttc ctacatgcaa ggtttcgccc acctggccca gcgccgccat gccgccgcgg
     7081 cggcatagga cgcaaggctt caggcgggga ccggtcccgc gccggaggcg agttcgtcga
     7141 gcagccatgc ccggaacagt tccagcgccg gatccccgca atgccgcggc gagctcatga
     7201 ggtaatacac ccgctcggac gccacttcgg ggggaaagat cttcaccagc gaaccgttct
     7261 tcagttcctc ctggatcagg aagctctgcg ccagggcgac gcccaggccc tgctgcgcgg
     7321 cgcggtaagc catg
//
LOCUS       DASWPO010000235         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2111193, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000235 DASWPO010000000
VERSION     DASWPO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111193"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGC69_12375"
     CDS             complement(<1..251)
                     /locus_tag="VGC69_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296119.1"
                     /translation="MHHCSPRSLSRKTALAWALAAAIGVTAAAGTAQAARLGHARVVS
                     APGTPLQVLVPLLDLTPDETTGLQVSLADAAAWQQAGLQP"
     gene            complement(393..1523)
                     /gene="asd"
                     /locus_tag="VGC69_12380"
     CDS             complement(393..1523)
                     /gene="asd"
                     /locus_tag="VGC69_12380"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY0296120.1"
                     /translation="MKQAVGLVGWRGMVGSVLMQRMREENDFAHLDPVFFSTSNAGGS
                     APDWADGAGPLQNAYDIEALKKLPIVVTAQGGDYTTAVYGKLRNAGWKGLWIDAASTL
                     RMADDAIIVLDPVNRPVIDAALKRGVRNFIGGNCTVSCMLMGLAGLFNNDLVEWMTTM
                     TYQAASGGGAQHMRELLTQFGLLNGAVKPLLDNPASAILDIDRGVLAKQKDAGLPQEQ
                     FGVPLAGNLIPWIDKDLGNGMSREEWKGEAETNKILGRGTAFGTPATPIDGLCVRIGA
                     MRCHSQALTIKLERDLPIDEIEGILAEGTQWAKVIPNTKEDTVKALTPVAVTGTLDIP
                     VGRLRKLSMGPQYLGAFTVGDQLLWGAAEPLRRMLRIALAEA"
     gene            complement(1651..>1965)
                     /locus_tag="VGC69_12385"
     CDS             complement(1651..>1965)
                     /locus_tag="VGC69_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY0296121.1"
                     /translation="MLTGSIGMLPSASLNESAQGLYEPSHGSAPDIAGQGIANPLATI
                     LSAAMLLRYSLDQAAQADRIEAAVRKVLAQGLRTADIYEPGTTKVSTAQMGDAVLKAL
                     GA"
BASE COUNT          321 a          583 c          717 g          344 t
ORIGIN      
        1 ggctgcaggc cggcttgctg ccaggccgcg gcgtcggcca gcgagacctg caggccggtg
       61 gtttcgtcag gcgtgagatc gagcaagggc accagcactt gcagcggcgt ccccggcgcc
      121 gacaccacgc gggcatgccc caggcgggcg gcttgcgcgg tgcccgcggc ggcggttacg
      181 ccgatggcgg cggccagcgc ccaggccagg gcggtcttgc gagacaggct gcgaggagaa
      241 caatgatgca tatcgcggtc atacccgatc atgagcgaat gttccaaatg ccaaaaagtc
      301 agcttgaaag tgtagacgta tcagtaatcc ggcacaaata aagcggggta cccaacggta
      361 ccccttggta agcaaaaacc gaatccgctg gatcaggctt cggccagcgc gatgcgcagc
      421 atgcggcgca gcggttcggc ggcgccccac agcaactggt cgcccacggt gaaggcgccc
      481 aggtattgcg ggcccatgga aagcttgcgc aggcggccca ccgggatgtc gagcgtgccg
      541 gtgacggcga cgggcgtcag cgccttgacg gtgtcttcct tggtattggg gatgaccttg
      601 gcccactggg tgccttcggc gaggatgcct tcgatttcgt cgatcggcag gtcgcgctcg
      661 agcttgatgg tgagcgcctg gctgtggcag cgcatggcgc cgatacgcac gcacaggccg
      721 tcgatgggcg tggcgggcgt gccgaaggcg gtaccgcggc cgaggatctt gttggtttcg
      781 gcctcgccct tccattcttc gcgggacatg ccgttgccga gatccttgtc gatccagggg
      841 atgaggttgc cggccagggg cacgccgaac tgttcttgcg gcaagccggc gtctttttgc
      901 ttggcaagca cgccgcggtc gatgtcgagg atggcggaag cggggttgtc gagcaacggc
      961 ttgaccgcgc cgttgagcag gccgaactgg gtgagcagct cgcgcatgtg ctgggcgccg
     1021 ccgccggaag cggcctggta ggtcatggtg gtcatccact cgaccaggtc gttgttgaag
     1081 aggccggcca ggcccatgag catgcagctg acggtgcaat tgccgccgat gaagttgcgc
     1141 acgccgcgct tgagcgccgc gtcgatcacc gggcggttga cgggatcgag cacgatgatg
     1201 gcgtcgtcgg ccatgcgcag ggtgctggcg gcgtcgatcc acaggccctt ccagccggca
     1261 ttgcgcagct tgccgtagac ggcggtggtg tagtcgccgc cttgggcggt gacgacgatg
     1321 ggcagttttt tgagcgcttc gatgtcgtag gcgttttgca gggggccggc gccgtcggcc
     1381 cagtccggcg cggaaccgcc ggcattgctg gtggagaaga acacggggtc gaggtgggcg
     1441 aagtcgtttt cttcgcgcat gcgctgcatg agcacggaac cgaccatgcc gcgccagccg
     1501 acgaggccta ctgcttgctt catttggaag aactcttaag gaaaaacagg aaatgactga
     1561 ttttccccaa ctttatcaga aagttggagt tgttgcggcg ccgcaaacag ggccggaagt
     1621 ccatccggcc ccgtgtgttg cctgccggac tcaggcgccc agggccttga ggacggcgtc
     1681 gcccatctgg gcggtggaca ccttggtggt gccgggctcg tagatgtccg cggtgcgcag
     1741 gccctgggcc agcaccttgc gcacggccgc ctcgatgcgg tcggcctggg cggcctggtc
     1801 cagcgaatag cgcagcagca tggccgccga caggatggtg gccagcgggt tggcgatgcc
     1861 ctggcccgcg atgtccggcg ccgagccgtg gctgggctcg tacaggccct gggccgactc
     1921 gttgagcgag gccgagggca gcatgccgat ggaacccgtg agcat
//
LOCUS       DASWPO010000236        17735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3000765, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000236 DASWPO010000000
VERSION     DASWPO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 17735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17735
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3000765"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /gene="pyrF"
                     /locus_tag="VGC69_12390"
     CDS             complement(<1..604)
                     /gene="pyrF"
                     /locus_tag="VGC69_12390"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY0296122.1"
                     /translation="MTFLQKLQDAWTSSGSLLQVGLDPDPQRFPADLQGKPDAIFQFC
                     RGIVDATAPYASSFKPQIAYFAAHRAEEQLEALCRHIRTAHPARPIVLDAKRGDIGST
                     AENYAREAYERYAADAVTVSPYMGLDSIEPYLAWQDRGVIVLCRTSNPGGSDLQFLKM
                     ADGQPLYLHVAGLVADKWNRGGQCGLVVGATFPNELAAVRR"
     gene            complement(646..1965)
                     /locus_tag="VGC69_12395"
     CDS             complement(646..1965)
                     /locus_tag="VGC69_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0296123.1"
                     /translation="MNSPIQPSAPTRVLIVGGGAGGLELAARLGDELGPGSVTLADSR
                     PFHIWKPSLHEAAAGTLDIHQEGLSYLMLAHMRGFTFAQGELLAVDRERRAARLGAVT
                     DAAGQTVLPERELPYDVLVLAIGSISNFFNTPGAAEYAVTLDSTEAAEAFRLTMLKAM
                     AQVDQAKERDPQARLRLVIVGGGATGVELAVELREASHIVSAYGLKRFQPEHDLAITL
                     VEGAPRILSPLSEKISRAALARLVSLGIQVEAQCKVSEITATEVRTADGRAFPTDLCM
                     WAAGIQGPPVLAGLGLPLNRLGQLQVDERLESPDPHILALGDCCAVPWTDGRNVPARA
                     QAAHQQASYLAGKILARAQGRPEPAAPYVYHDNGSLVSLGREAGVGSLMGKLSGRGLF
                     VSGTLARLMYMSLHLMHHRAVLGFWRTAALALARLLMRRTRPRVKLH"
     gene            complement(2023..2427)
                     /locus_tag="VGC69_12400"
     CDS             complement(2023..2427)
                     /locus_tag="VGC69_12400"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY0296124.1"
                     /translation="MQPPESSAKSPYKSKGGLRRILNALRYSLQGLKAAIQYEAAFRQ
                     ELGLAILLVPAAFFLGRTTTEVFILIMSVMLVLMAELLNSAVEAVADALSVEINPLLG
                     RAKDLGSAAVMLMLIFTLAVWAAVAISRFILN"
     gene            complement(2529..4307)
                     /locus_tag="VGC69_12405"
     CDS             complement(2529..4307)
                     /locus_tag="VGC69_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0296125.1"
                     /translation="MNTSSTASLPARSAASSSRPFRPDVLWLALPAALFLLVFLAYPA
                     VKVLALSLVQNNGSAFSTAAFERFFGPSVYNRVLGTTFSIAWQTTAWCLLCGYPLAYW
                     LSRLPARRQQLLALLVLLAFWTSSLVKNFSWLVLLGRTGFMATLLQAIGIPGGDHLLF
                     NRAVVIFAMVHTLLPLGVVTMLPVMNQVDQRCMSAAATLGADRAQMFWRVFLPLSMRG
                     VASAGLLVFIGALGFFITPTLLGSPKEMMLGQLVIVQINELQNWQMGCALAVVLVLTA
                     LVCCVVYDLVFGLSSLAGGGAGQAGSSRQKWLRTLGIALANGLGTACSRLLRLYEVQP
                     LRLPLLNLYSILVIVVLLFPIVAIVPMAFTDSTFLSFPPKGFSLRWFAAYFDSPLWMA
                     ATVRSFGIGLATALITSVFATLAALAVLRSSSRLRQPAFLLFLTPMMVPSIVIAIGMF
                     YLCAHIGLVATNAGITLGHIVIAMPIMFVIMLSTFKGHDWGLDSAAATLGANRLQVLR
                     RVTLPLVKAGLAASFVIGFLTSFEELTVALFIGGGIKSTMPKQMWTDIILQTTPLLAA
                     ASTVVLIIVTLLFLAAHTLRPRGSRT"
     gene            complement(4304..5644)
                     /locus_tag="VGC69_12410"
     CDS             complement(4304..5644)
                     /locus_tag="VGC69_12410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0296126.1"
                     /translation="MSKKHIPLATLVHATGSHPASWLQASAPRGASTNIEHYRSLAQL
                     AEQGLMDLFFIADTPAARTNRLEAFSRYPMFMNVFEPITLLSALAGSTSHIGLGATAS
                     TSFYEPYNLARLFASLDHISHGRAAWNVVTSANDYAARNFGLDKLPPHDRRYEKAAEC
                     YDIVDELWDTWDDDAFIYDRADGRVFDPAKQHVTDHKGEFFQVHGALNIARAPQGKPV
                     IIQAGSSEAGKEFAARTAEVVFASDPTLEKSQQFYADLKGRMGKFGRSPDSMKILAGL
                     SVIVGDSKTEAQAKYRTLQELIHPAVGLMRLGDDLEMDLTKLPLDEPIPVSMIPEKSN
                     LHQAYFNQIATLIREQKLTLRQMYTSYERGKVTLCGTPAEIVDEMQNWVESQACDGFM
                     LSFHVLPDHMTDFVRRVTPELQKRGLFRSAYEGKTLRDNLGLARPANRYAKKAE"
     gene            complement(5682..6752)
                     /locus_tag="VGC69_12415"
     CDS             complement(5682..6752)
                     /locus_tag="VGC69_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296127.1"
                     /translation="MSLDFSNRRRRFIKTTAAGLATIAAPAIWVPTARAAGKQITVAD
                     NGGALQPVMRKAFYDPFEKATGIQVVNVAHESDPVTAFKLSVDSGSNIWDLCMVTPDH
                     VARLSAPKNYLAPLNIADADAKGILPGMLTGNWFGFSVYGVLMAYRSDKYPNGPTGWH
                     DFWDTDKFPGRRGLYRSPIGMMENALLADGVALKDLYPVDVDRALKALDRIRKSVAVW
                     WTNGAQNTQILQSGEVDMADTWNARALAAIEAGAPVKIVWEGTYSTDGWSIPISTPRV
                     EEARQFVRFCMQPERQAEYSSLISNGPSNGDAYKFIDAKRAELLPTYPKNFERLAKRD
                     AAWWGKNYDAVNEKFQEWLLSA"
     gene            complement(6831..7913)
                     /locus_tag="VGC69_12420"
     CDS             complement(6831..7913)
                     /locus_tag="VGC69_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296128.1"
                     /translation="MASVSPYLSMQALDSSGIKMRAEGLSKHYGDTVALHPTDLELHS
                     GELLTLLGPSGSGKTTLLQLISGLVPPTSGKLYIDGLDRTHAPVHQRDIGVVFQNYAL
                     FPHLTVYENIAFPLQMRGIDAASMRVQIGDALEMVNMETLLDRYPRELSGGQQQRVAL
                     ARCLVYRPSLILMDESLSALDRKLRENMQVEIKRIHRETGSTIIFVTHDQEEALALSD
                     RVCLMSTGRIEQIGTPEDIYERPRTTFVADFIGISNKLHGTIADNCLATADGRIPLAP
                     PASVSGEATLIVRPEHVRLADPGQGLVHGQVIERIYGGSGSRVMVRLNSGAVMTVLQA
                     PRGAQCVIGDTVSLAWQAEDARLIQH"
     gene            complement(8111..9022)
                     /locus_tag="VGC69_12425"
     CDS             complement(8111..9022)
                     /locus_tag="VGC69_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0296129.1"
                     /translation="MPVLSHFRLPRLAELGGMMADCAREWTRHRASSKGAALALYMVF
                     SLAPMLILVIAVAGFFFGESAVRSELFSQVRDLMGERGADIIQTVLASAHESNSGWIA
                     ALLSISVLIFSATTAFTELKASLDELWEVQPSAKNALRGMVQSRMASFGLVLVLALFL
                     LLSLMINAGLAATRRYYGDLWSYSTFVLAADWLSNLLSFSLVTALFAVVFKLLPSLRI
                     AWLDVIPGAIVTSALFLVGKWGIGLYLSHGGTVSAYGAAGSLIALLLWIYYSAQIFFF
                     GAVFTRQFALRFSRSFSQQHAAPTTPS"
     gene            complement(9140..10780)
                     /gene="groL"
                     /locus_tag="VGC69_12430"
     CDS             complement(9140..10780)
                     /gene="groL"
                     /locus_tag="VGC69_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938027.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0296130.1"
                     /translation="MAAKQVLFGDDARVRIVRGVNILANAVKTTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENIGAQLVKDVASKTSDNAGDGTTTATVLAQAIVQE
                     GLKYVAAGFNPIDLKRGIDKAVTAAVAELKKISKPVTTSKEIAQVGSISANSDHSIGQ
                     IIADAMDKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINNPDKQVAALEDP
                     FVLIYDKKISNIRDLLPVLEQVAKSSRPLLIIAEDVEGEALATLVVNNIRGILKTTAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEETGLSLEKATLAELGQAKRIEVAKENTTIID
                     GAGDGKSIEARVKQIRVQIEEATSDYDREKLQERVAKLAGGVAVIRVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALLRAKQAIANLKGDTPDQNAGIKLILRAVEEP
                     LRTIVANAGEEASVVVNNVLNGKGNFGYNAATGEYVDLVEQGVLDPTKVTRTALQNAA
                     SVASLLLTAEAAVVELVEDKPAAPAMPGGMGGMGGMDF"
     gene            complement(10854..11141)
                     /locus_tag="VGC69_12435"
     CDS             complement(10854..11141)
                     /locus_tag="VGC69_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0296131.1"
                     /translation="MALRPLHDRVIVKRLDNERKTASGIVIPESAAEKPDQGEVMAVG
                     PGKKTEDGKILPVDLKVGDKVLFGKYAGQSVKVDGEELLVIREEEILAVIL"
     gene            complement(11505..12467)
                     /locus_tag="VGC69_12440"
     CDS             complement(11505..12467)
                     /locus_tag="VGC69_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296132.1"
                     /translation="MKRCITVLLLCLGLCGALRAAPVWPTKRILVIVPYAAGSTPDLI
                     ARLIFDQVGQSTGQPVIVENKPGAGGMIGADFVAKSTPDGSTLLLAPSGPLETDALLY
                     KKMTYDPVKDLAPVALVAETPSVVVTSNAVSAPDARALLRDMADASRKLAYASPGYGT
                     LSHLSTAYLVARSGGNVPHVAYPGSPQVVTGLIANDVQIATLPPLAVAPFIRAGKIRA
                     VALVGTHRSDALPDVATLREQGVDFDPVGWFGVAAPGGTPAPVLEAVHDAISAALKNS
                     ALVQAYRAQGLEVADLGPDAFAAYIRKDLVQWRPVIEQNHISLD"
     gene            complement(12512..13348)
                     /locus_tag="VGC69_12445"
     CDS             complement(12512..13348)
                     /locus_tag="VGC69_12445"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0296133.1"
                     /translation="MAGRGFALIRGVPVQRLSDEEARLAYWGIGLLFGVGLSQNAKAD
                     FLCPVTDMGVDFGYSGAASQYNVRGYQSRADLNYHCDPTDVVALLCLRQARSGGISTI
                     VSTPAIFNEIRAGHPEHLPVLLRGFAYDRKGEGWAAEAPVTPRIPVFVHHGGRVSCRY
                     ARSYIQGAAQKLGQALDDDERAALDCFDAIARREDMAMHMEFRPGDIQLLNNHTVVHG
                     RTAYEDDPDPARRRFLYRLWLQIDRAPWTEEDEVMRWAFARFGNLGRTAEQWRALQDS
                     TR"
     gene            complement(13349..13588)
                     /locus_tag="VGC69_12450"
     CDS             complement(13349..13588)
                     /locus_tag="VGC69_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296134.1"
                     /translation="MQERDAMQQALPPVAGPSAWRPADFVQDHSWCHELSQDQAEEVA
                     RAAQRWRGRAFDSLTREEAGQALAGLASLASRVTG"
     gene            13690..14586
                     /locus_tag="VGC69_12455"
     CDS             13690..14586
                     /locus_tag="VGC69_12455"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296135.1"
                     /translation="MNLRYLEVFRLVMQTGTIKGAAALMHVSEAAVSKLLGTAERRMG
                     LALFERVKGRLIPTPEARRLYEEVELLWTRVERIENLTASLANPDGGSLTVAISPSLG
                     TTVAPAAATALLARLPRATINIELLIPQLLVQALVDGIAELGVSLSPQEHPSLQVLQR
                     YPCGLACVMPRGHPLAKKRVIGPADLRGHAVVSFPQALAYGITEAQLYGSHADEINVR
                     LNVRSGQTACWFSLAGAGVAIVDAATVAGQAFPDLAVRPYRSQARLALHVLRHRNRPL
                     SRAGIIFCETLDETWKRLGKEG"
     gene            complement(14630..15466)
                     /locus_tag="VGC69_12460"
     CDS             complement(14630..15466)
                     /locus_tag="VGC69_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HEY0296136.1"
                     /translation="MKTPKRLMPLLQEGLIDEVLGQLMSGKEATVYTVRCGESVRCAK
                     VYKDVKQRSFRQAASYLDGRKVKNSREARALEKGTRYGRQVQEKLWQTAEVDALFLLA
                     NAGVRVPQPYICTDGVLLMELVTDEAGNVAPRLGDVAMTETQALDLHARLLDQVVRML
                     CAGLIHGDLSEYNTLLAADGPVIIDLPQAVNAAGNLEARAMLERDVDNLKVFFGQAAP
                     QLLTTHYGKEIWGLFERGGLHVGVALTGHVEIDTTPVALDALLDDIEDARLEMEAALN
                     GR"
     gene            complement(15584..16084)
                     /locus_tag="VGC69_12465"
     CDS             complement(15584..16084)
                     /locus_tag="VGC69_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296137.1"
                     /translation="MNDDQIPDTDIPEDRKTQEPRLWRNDGWTARVIKNEDDDGWAVE
                     MTQDGEAEPALVGPWTMGRDKKNPKPLDAPAFFTLVKTANEVLRRHEQQLHATLHKSV
                     KIDAAGGRLTIKLDIIPDEDDPYALLSAYDESGEQLAQIRVLPTYKLGVQRASEWAAG
                     GFGNGG"
     gene            complement(16107..16409)
                     /locus_tag="VGC69_12470"
     CDS             complement(16107..16409)
                     /locus_tag="VGC69_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0296138.1"
                     /translation="MRTYNHPDPANFVLERIFHALSDPVRLEIVQRLAHVEEATCGEL
                     DGGRPKSSMSHHFRILREAGLVQTSVAGTVHQNTLRRAELNSRFPGLVDAILRQMA"
     gene            16499..17596
                     /locus_tag="VGC69_12475"
     CDS             16499..17596
                     /locus_tag="VGC69_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY0296139.1"
                     /translation="MSSLFTPYKLKDVSLRNRIAVPPMCQYSADDGLINDWHRVHLAG
                     LARGGAGLVIVEATAVSPEGRITPGCAGLWNDEQAQAFVPAVASIKAAGAVPGIQIGH
                     AGRKASANRPWEGDDHIAADDPRGWPTIAPSAIAFGEGLSKMPSAMSLDDIARVRQDF
                     VAAAKRARDAGFEWLELHFAHGYLGQSFFSPHSNQRNDAYGGSLENRSRFLLETLAAV
                     RKVWPEHLPLTARFGVIEFDGRDEETLAESIELARNFKREGLDMLSVSMGFSTPTANI
                     PWAPALLAPIAERVRREADIPVSSAWGIDTPELADGAVKHGQLDLVMVGRAHLADPHW
                     PYYAARKLGIERPSWTLPAPYAHWLERYAVN"
BASE COUNT         2950 a         5566 c         6128 g         3091 t
ORIGIN      
        1 gccggcgcac cgccgccagt tcgttgggga aggtggcgcc caccaccagg ccgcactgcc
       61 cgccgcggtt ccatttgtcg gccaccaggc cggccacgtg caggtacagg ggctggccgt
      121 cggccatctt caggaactgc aggtcggagc cgccgggatt ggacgtgcgg cacagcacga
      181 tcacgccgcg gtcttgccag gccaggtagg gctcgatcga gtccaggccc atgtaggggc
      241 tgaccgtgac ggcgtcggcc gcatagcgtt cgtaggcctc gcgcgcgtag ttctcggccg
      301 tggagccgat gtcgccgcgc ttggcgtcga gcacgatggg ccgggcggga tgggcggtgc
      361 ggatgtggcg gcacagcgcc tcgagctgct cttcggcgcg gtgcgcggcg aagtaggcga
      421 tctgcggctt gaaactgctg gcgtagggag ccgtggcgtc gacaatgccg cggcagaact
      481 ggaaaatggc gtcgggcttg ccctgcagat cggcggggaa gcgttgcgga tcggggtcca
      541 ggcccacttg cagcagcgag ccgctggagg tccaggcgtc ttggagtttt tgcaggaacg
      601 tcatcgggcg agaaagtggt ggccggccag atgggggtcg gcctgctagt gcagcttgac
      661 gcggggccgg gtgcgccgca tcagcaggcg ggccagcgcc agggcggcgg tgcgccagaa
      721 cccgagcacg gcgcggtggt gcatcaggtg caggctcatg tacatcaagc gcgccagtgt
      781 accgcttacg aaaagcccgc gcccgctgag cttgcccatc aggctgccca cgccggcctc
      841 ccgccccagc gacaccagcg agccgttgtc gtggtagaca tagggcgcgg ccggctcggg
      901 ccggccttgc gcgcgtgcca ggatcttgcc ggccagatag ctggcctgct ggtgcgccgc
      961 ctgcgcccgg gccggcacgt tccggccatc cgtccacggc accgcgcagc agtctcccag
     1021 ggccaggatg tgcggatcgg ggctttccag ccgctcgtcc acctgcagct ggcccagccg
     1081 gttcagcggc aggcccagcc cggcgaggac gggcggcccc tggatgccgg cggcccacat
     1141 gcagaggtcg gtgggaaagg cccggccgtc ggccgtgcgc acctcggtcg cggtgatttc
     1201 cgagaccttg cactgggcct ccacctggat accgagcgag accagccgcg caagggcggc
     1261 gcgcgagatc ttctcggaca gcggcgacag gatgcgcggc gcgccctcga ccagggtgat
     1321 ggccaggtcg tgctcgggct ggaagcgctt gagcccgtag gcgctgacga tatggctggc
     1381 ttcgcgcagt tccacggcca gctccactcc cgtggcgccg ccgcccacga tgaccaggcg
     1441 caggcgggcc tgcggatccc gctccttggc ctggtccact tgggccatgg ccttgagcat
     1501 ggtcagccgg aaagcctcgg ccgcctcggt ggagtccagg gtgacggcgt actcggccgc
     1561 gccgggcgtg ttgaaaaagt tggagatgct gccgatggcc agcaccagga cgtcgtaggg
     1621 cagctcgcgc tcgggcagca cggtctgtcc cgcggcgtcg gtgacggcgc ccaggcgcgc
     1681 cgcgcgccgt tctcggtcga cggccagcag ctcgccctgg gcgaaggtga agccgcgcat
     1741 gtgcgccagc atcaggtagg acaggccttc ctggtgaatg tccagcgtgc cggcggcggc
     1801 ttcgtgcagc gagggcttcc agatgtggaa gggccggctg tcggccagcg tgacgcttcc
     1861 cggcccgagt tcgtcgccga gccgggcggc cagttccagg ccgcccgcgc cgccgcccac
     1921 gatgaggacc cgggttggcg cgctgggctg gattggggag ttcatggtcg cagtatatga
     1981 gctggatcgt tgaggattcc tggccgcgcg gtcgacgggc ggctagttca ggatgaagcg
     2041 gctgatcgcc acggcggccc agaccgccaa ggtgaaaatc agcatcagca tcaccgccgc
     2101 gctgcccagg tccttggcgc ggcccagcag cgggttgatc tcgaccgata gcgcgtcggc
     2161 caccgcctcg acggcggaat tgagcagctc ggccatcagc accagcatga ccgacatgat
     2221 caggatgaat acctcggtcg tggtgcggcc caggaaaaag gcggcgggca ccagcaggat
     2281 ggccaggccc agctcctgcc ggaaagcggc ctcgtactgg atggcggctt tcaggccttg
     2341 cagcgaatag cgcagggcat tgaggatgcg gcgcaggccg cccttgctct tgtacggtga
     2401 cttggcggag gattcggggg gctgcatgga agactccagc ggaaaccggg atcggctggg
     2461 ctgagtttta acagatatgc cagccggaat ccgttatatc gcagccgtac gtgccaggat
     2521 gcgcgatttt aggtgcggga gccacgcggg cgcaaggtat gcgccgccag gaagagcagc
     2581 gtcacgatga tcagcaccac ggtcgaggcg gcggccagca gcggggtggt ctgcaggatg
     2641 atgtcggtcc acatctgctt gggcatggtg ctcttgatgc cgccgccgat gaagagcgcc
     2701 accgtcagct cctcgaacga ggtcaggaag ccgatcacga acgaggcggc caggccggcc
     2761 ttgaccagcg gcagggtcac gcggcgcagc acttgcaggc gattggcgcc cagcgtggcg
     2821 gcggcgctgt ccagccccca gtcgtggccc ttgaaggtgc tcagcatgat gacgaacatg
     2881 atgggcatgg cgatcacgat atggcccagc gtgatgccgg cattggtggc caccaggccg
     2941 atgtgcgcgc acaggtagaa catgccgatg gcgatgacga tgctgggcac catcatgggc
     3001 gtgaggaaca gcaggaaggc cggctggcgc agccggctgc tcgagcgcag cacggccagc
     3061 gcggccagcg tggcgaacac gctggtgatg agcgccgtgg ccagcccgat gccgaaggac
     3121 cgcaccgtgg ccgccatcca cagcggcgaa tcgaaatacg ccgcgaacca gcgtaggctg
     3181 aagcccttgg gcgggaagga caggaaggtg ctgtcggtaa acgccatggg cacgatggcc
     3241 acgatgggaa agagcagcac cacgatcacc aggatgctgt agagattgag cagcggcagg
     3301 cgcagcggct ggacttcata gaggcgcagc agccgcgaac aggccgtgcc caggccgttg
     3361 gccagggcga tgcccagcgt gcgcagccac ttctggcgcg acgatcccgc ctggccggct
     3421 ccgccgccgg ccagcgacga caggccgaag accaggtcgt acaccacgca gcagaccagg
     3481 gccgtgagca cgagcaccac ggccagggcg cagcccatct gccagttctg cagctcgttg
     3541 atctgcacga tgacgagctg gcccagcatc atttccttgg ggctgcccag cagcgtgggc
     3601 gtgatgaaga agcccagggc gccgatgaag accaagagcc cggccgaggc cacgccgcgc
     3661 atcgacagcg gcaggaacac ccgccagaac atctgcgcgc gatcggcgcc cagcgtggcg
     3721 gcggcgctca tgcagcgctg gtcgacctgg ttcatcaccg gcagcatggt gaccacgccc
     3781 aggggcagca gggtatgcac catggcgaag atcaccacgg cgcggttaaa gagcagatgg
     3841 tcgccgcccg ggatgccgat ggcctgcagc agcgtggcca tgaagccggt gcggcccagc
     3901 agcaccagcc aggaaaaatt cttgaccagc gaactcgtcc agaaggccag cagcaccagc
     3961 agcgccagca gctgctggcg gcgcgcgggc aggcgcgaca gccagtaggc cagcgggtag
     4021 ccgcacagca ggcaccaggc cgtggtctgc caggcgatgg agaaagtggt gcccagcacc
     4081 cggttgtaga ccgacgggcc gaagaagcgc tcgaaggccg ccgtcgagaa ggcgctgccg
     4141 ttgttctgca ccaggctcag cgccagcacc ttgacggccg gataggccag gaacaccagc
     4201 aggaacagcg cggccggcag ggccagccac aggacgtcgg gccggaaggg gcgcgacgag
     4261 gatgcggccg agcgggccgg cagtgaagcg gtactgctgg tgttcattcg gcttttttgg
     4321 cgtagcggtt ggcgggacgc gccaggccga ggttgtcgcg cagggtcttg ccttcgtagg
     4381 cgctgcggaa caggccgcgc ttttgcagct cgggcgtgac gcgtcgcacg aaatcggtca
     4441 tgtggtcggg cagcacgtgg aacgacagca tgaagccgtc gcaggcctgg gactcgaccc
     4501 agttctgcat ttcgtcgacg atctcggcgg gcgtgccgca cagcgtgacc ttgccgcgct
     4561 cgtagctggt gtacatctgg cgcagcgtga gcttctgctc gcggatcagg gtggcgatct
     4621 ggttgaagta cgcctggtgc aggttggact tctcgggaat catggacacc gggatgggct
     4681 cgtccagcgg cagcttggta aggtccattt ccagatcgtc gcccaggcgc atcaggccca
     4741 ccgcggggtg gatgagctcc tgcagcgtgc ggtacttggc ctgggcttcg gtcttgctgt
     4801 cgcccacgat caccgacagg ccggccagga tcttcatcga gtcgggcgag cggccgaact
     4861 tgcccatgcg gcctttcagg tctgcataga actgctgcga tttctccagc gtggggtcgg
     4921 aggcgaacac cacttcggcg gtgcgggcgg cgaattcctt gcccgcctcg gacgagccgg
     4981 cctggatgat caccggcttg ccctgcggcg cgcgcgcgat gttcagcgcg ccgtgcacct
     5041 ggaagaactc gcccttgtgg tcggtgacgt gctgcttggc cgggtcgaac acgcggccgt
     5101 cggcgcggtc gtagatgaag gcgtcgtcat cccaggtgtc ccacagctcg tcgacgatgt
     5161 cgtagcactc ggccgccttc tcgtagcggc ggtcgtgcgg gggcagcttg tccaggccga
     5221 agttgcgcgc ggcatagtcg ttggccgagg tgaccacgtt ccaggcggcg cggccgtgcg
     5281 aaatgtggtc gagcgaggcg aacaggcggg ccaggttgta gggttcgtag aagctggtgg
     5341 acgccgtggc gcccaggccg atgtgcgagg tcgagcccgc cagcgccgac agcagcgtga
     5401 tgggctcgaa cacgttcatg aacatcgggt agcggctgaa ggcttccagc cggttggtgc
     5461 gcgcggccgg ggtgtcggcg atgaagaaca agtccatcag gccctgttcg gccaattggg
     5521 caaggctgcg atagtgttcg atattggtcg aggcgccgcg cggcgcgctg gcctgcagcc
     5581 acgaagccgg atgcgagccg gtggcatgca ccagcgtggc cagggggatg tgctttttgc
     5641 tcatgacggg actcccggtg gggcggggaa ggcgcgggcg cttaggcgct cagcagccat
     5701 tcctggaatt tttcgttgac ggcgtcgtag ttcttgcccc accaggccgc gtcgcgcttg
     5761 gccaggcgct cgaagttctt gggataggtg ggcagcagct cggcgcgctt ggcgtcgatg
     5821 aacttgtagg cgtcgccgtt gctcgggccg ttggagatca ggctggagta ttcggcctgg
     5881 cgctcgggct gcatgcagaa gcgcacgaac tggcgggctt cctcgacgcg cggcgtgctg
     5941 atggggatgg accagccgtc ggtgctgtag gtgccttccc agacgatctt caccggtgcg
     6001 ccggcctcga tggcggccag cgcgcgggcg ttccaggtgt cggccatgtc cacttcgccg
     6061 ctttgcagga tctgcgtgtt ctgcgcgccg ttggtccacc acaccgcgac cgacttgcgg
     6121 atcctgtcga gcgccttcag ggcgcggtcc acgtcgaccg ggtagaggtc cttgagcgcc
     6181 acgccgtcgg ccagcagcgc gttttccatc atgccgatcg gggaacggta caggccgcgg
     6241 cggccgggaa atttgtcggt gtcccagaag tcgtgccagc cggtcgggcc gttgggatat
     6301 ttgtcgctgc ggtaggccat cagcacgccg tacaccgaga agccgaacca gttgccggtg
     6361 agcatgccgg gcaggatgcc cttggcgtcg gcgtcggcga tgttcagcgg cgccaggtag
     6421 ttcttgggcg cggacaggcg cgccacgtgg tcgggcgtga ccatgcagag atcccagatg
     6481 ttggagccgg agtccaccga cagcttgaag gccgtgaccg ggtcggactc gtgcgccacg
     6541 ttgacgacct ggatgccggt ggctttttcg aaggggtcgt agaaggcctt gcgcatcacg
     6601 ggctgcaggg cgccgccgtt gtcggccacg gtgatctgct tgcccgcggc gcgtgcggtg
     6661 ggcacccaga tggccggggc ggcgatcgtc gccaggccgg cggcggtggt tttgataaag
     6721 cgtcggcggc ggttcgagaa atcgaggctc actgcattct ccttgtgcgc tgggatgtcg
     6781 ggccgttcct tcgccgcgcc gcgaaagaaa gggggcaaga ttaaaggggg tcagtgctgg
     6841 atgagccgcg cgtcttcggc ctgccaggcc agcgacacgg tatcgccgat gacgcattgc
     6901 gcgccgcgcg gcgcttgcag cacggtcatg acggcgccgc tgtttaagcg caccatgacg
     6961 cggctgcccg agccgccgta gatgcgctcg atgacctggc cgtgcaccag gccctggccc
     7021 ggatcggcca gccgcacatg ctcgggccgc acgatgagcg tggcctcgcc ggacacggag
     7081 gcgggcggcg ccaggggaat gcggccgtcg gccgtggcca ggcaattgtc ggcgatggtg
     7141 ccgtgcagct tgttggagat gccgatgaaa tccgccacga aggtggtgcg gggacgttcg
     7201 tagatgtcct cgggcgtgcc gatctgctcg atgcggccgg tgctcatcag gcagacgcgg
     7261 tccgatagcg ccagggcttc ttcctggtcg tgcgtgacga agatgatggt cgagccggtt
     7321 tcgcggtgga tgcgcttgat ctcgacctgc atgttctcgc gcagcttgcg gtccagcgcc
     7381 gagagcgatt cgtccatgag gatgagcgag ggccgataca ccaggcagcg cgccagcgcc
     7441 acgcgctgct gctgcccgcc ggagagctcg cgcggatagc ggtcgagcag ggtttccata
     7501 ttgaccattt ccagcgcgtc gccgatctgc acgcgcatgc tggccgcgtc gatgccgcgc
     7561 atctgcagcg gaaaggcgat gttctcgtac accgtcaggt gcgggaacag ggcgtagttc
     7621 tggaacacca cgccgatgtc gcgctggtgc accggcgcgt gggtgcggtc gagcccgtcg
     7681 atgtacagct tgcccgaagt gggcggcacc aggcccgaga tgagttgcag cagcgtggtc
     7741 ttgcccgagc ccgaagggcc cagcagggtg agcagttcgc cgctgtgcag ctcgaggtcg
     7801 gtggggtgca gggcgacggt gtctccataa tgcttgctca agccttcggc ccgcatcttg
     7861 atgccggagg aatcgagcgc ttgcatagac aaatacgggg agacggaagc catgggaatc
     7921 tcaatgctgc tacatgttgt ctagacagga taaggcagcg ccgagaaaac cccaatatcg
     7981 cgttttcccc ggggtatcgc aaggcgattt gccatctcgc cgatcgtgag cggatacggt
     8041 gcggcctggc tcgatatggt gcgagaacgc accgggattg ggcggcccgg gctggtggcc
     8101 gcggcagctt tcagctcggt gtcgtggggg ccgcgtgctg ctggctgaag gaacggctaa
     8161 agcgcagggc gaactggcgg gtgaagacgg cgccgaagaa gaagatctgc gccgagtaat
     8221 agatccagag cagcagcgcg atcagcgagc ccgccgcgcc gtaggccgaa acggtgccgc
     8281 catggctcag gtacaggccg atgccccatt tgcccaccag gaagagcgcc gaggtgacga
     8341 tggcgccggg gatgacgtcc agccaggcga tgcgcaggct gggcagcagc ttgaacacca
     8401 cggcgaacaa ggcggtgacc agcgagaacg acagcaggtt cgacagccag tcggccgcca
     8461 gcacgaaggt ggaatagctc cagaggtcgc cgtagtagcg gcgcgtggcc gccagcccgg
     8521 cgttgatcat cagcgacaac agcaggaaga gcgccagcac cagcaccagg ccgaacgacg
     8581 ccatccggct ttgcaccatg ccgcgcagcg cgttcttggc gctcggttgc acctcccaca
     8641 gctcgtcgag gctggccttg agttcggtga aggccgtggt ggcggaaaag atcagcacgc
     8701 tgatcgacaa cagggcggcg atccagccgc tgttggactc atgggcgctg gccagcaccg
     8761 tctggatgat gtcggcgccg cgctcgccca tcaggtcgcg cacctgcgag aacagttcgg
     8821 agcgcaccgc cgactcgccg aagaaaaagc cggccacggc gatcaccagg atcagcatgg
     8881 gcgccagcga gaacaccatg tacagggcca aggcggcgcc cttgctcgag gcgcggtggc
     8941 gggtccattc cctggcgcag tcggccatca tcccgccgag ttcggcaagg cggggcaggc
     9001 ggaagtgcga cagtacgggc atgggtccaa cgtccagttc gccaggcggc gggttaagaa
     9061 ccgatcatcg cttgcatcat aaagaaaaaa ccccgcaggg cgaacctgcg gggtttcgtg
     9121 agggcaggaa ggccgggact tagaagtcca tgccgcccat gccacccatg ccgccgggca
     9181 tggccggggc agcgggcttg tcttcgacca gctcgaccac ggcggcttcg gcggtcagca
     9241 gcaggctggc gaccgaggcg gcgttttgca gggcggtgcg ggtcaccttg gtgggatcca
     9301 gcacgccttg ctcgaccagg tcgacgtact cgccggtggc cgcgttgtag ccgaagttgc
     9361 ccttgccgtt gagcacattg ttcaccacca cgctggcttc ttcgccggcg ttggcgacga
     9421 tggtgcgcag gggctcttca accgcgcgca ggatcagctt gatgccggcg ttctggtcgg
     9481 gggtgtcgcc cttcaggttg gcaatggctt gcttggcgcg cagcagggcc acgccgccgc
     9541 cagccaccac gccttcttcc accgcggcac gggtcgcgtg cagggcgtct tcgacgcggg
     9601 ccttcttttc cttcatttcg acttcggtgg cagcgcccac gcgaatcacg gcaacaccgc
     9661 cggccagctt ggccacgcgt tcctgcagct tttcacggtc gtagtccgag gtggcttctt
     9721 cgatctgcac gcggatttgc ttgacgcggg cttcgatcga cttgccgtcg ccggcgccgt
     9781 cgatgatcgt ggtgttttcc ttggccactt cgatgcgctt ggcctggccc agctcggcca
     9841 gggtcgcctt ctcgagcgac aggccagtct cttccgagat cacggtgccg ccggtcagga
     9901 tggcgatgtc ttcgagcatg gccttgcggc ggtcgccgaa gccaggcgcc ttgacggcgg
     9961 tggtcttcag gatgccgcgg atgttgttca caaccagggt ggccagggct tcgccttcga
    10021 cgtcttcagc gatgatcagc agaggacggc tcgacttggc gacttgttcc agcacgggca
    10081 gcaggtcgcg gatgttgctg atcttcttgt cgtagatcag gacgaaagga tcttccagcg
    10141 cggcgacttg cttgtcgggg ttgttgatga agtagggcga caggtagccg cggtcgaatt
    10201 gcatgccttc gacgacgtcc agctcgtttt ccagcgactt gccgtcttcg acggtgatga
    10261 cgccttcctt gccgaccttg tccatcgcgt cagcgatgat ctggccgatg gagtggtcgc
    10321 tgttggccga gatcgagccg acctgggcga tttccttgct ggtggtcacc ggcttggaga
    10381 tctttttcag ttcggccacg gcggcggtca cggccttgtc gatgccgcgc ttcagatcga
    10441 tggggttgaa gccagcggcg acgtacttca ggccttcctg cacgatggcc tgggccagca
    10501 cggtggcggt ggtggtgccg tcaccggcgt tgtcggaggt cttggaggca acgtccttga
    10561 ccagttgggc gccaatgttt tcgaacttgt ccttcagttc gatttccttg gcgacggaca
    10621 caccgtcctt ggtgacggtg ggggcgccga acgagcgctc caacacgaca ttgcggccct
    10681 tggggcccag ggtggtcttg acggcgttgg cgaggatatt cacgccacgg acaatgcgca
    10741 cgcgggcgtc atcgccgaac agaacttgtt tggcagccat ttctcagctt ccttcgttga
    10801 attcgagggg gttgaccatg gatcgatcac gcggtgatcg atcggggcag caattacagg
    10861 atcacggcga ggatttcttc ctcgcggatg acgagcaact cttcgccatc gaccttgacg
    10921 ctctggccgg catacttgcc gaacagcacc ttgtcgccga ctttcaggtc gaccggcagg
    10981 atcttgccgt cttcggtctt cttgccggga ccgacagcca tcacttcgcc ttggtcgggc
    11041 ttttcagccg cgctttcggg gatgacgatg cccgaggcag ttttgcgctc gttgtccagg
    11101 cgtttgacga tcacgcgatc gtgcagggga cgcagggcca tttggaactc ctaactttgt
    11161 aaagaaaagc caatgggaat tgcttggggg tgacccggga gcctggctgc caggcggagt
    11221 ctccgaccgg tggcagccgg aggtgttagc actcaccggt gacgagtgct aattataggg
    11281 gcgagacggg gaggatcaag tggggggagc agggattgtt acaaattggt agcgaatgcc
    11341 gaagttgtcg aaatgtcgaa agcccagccg aagtggaatt ttggtcagtt cgtaatctcc
    11401 ccccaaacag tagacgacag ccggtttctc taggctggcc ctgtccactt ctggggggag
    11461 gtcagcagcg gctcgtagcc gcggagcacg cctggctttc cgccctagtc cagcgaaata
    11521 tggttctgct cgatgaccgg ccgccattgc acaagatcct tccggatata ggccgcgaac
    11581 gcatccggcc cgagatcggc cacctccagg ccttgcgcgc gatacgcctg gacgagcgcg
    11641 gagttcttca gggcggccga gatcgcgtcg tgcacggctt ccaggacagg tgcgggcgtg
    11701 ccgccgggcg cggccacgcc gaaccatcca acgggatcga aatccacccc ctgctcacgc
    11761 agcgtggcca cgtccggcaa ggcatcgctg cgatgcgtgc ccaccaatgc cacggcgcgg
    11821 atcttccccg cgcgaataaa gggcgcgacc gccagcggcg gcaaggtcgc gatctggaca
    11881 tcattggcga tgagccccgt cacgacttgt ggcgaccccg ggtaagccac atgaggcaca
    11941 ttgccgcccg aacgggcgac cagataggcc gtgctcaggt ggctcaacgt gccgtagccg
    12001 ggcgaggcgt aggccagttt ccgggacgcg tccgccatgt cgcgcagcaa ggcccgggcg
    12061 tccggcgctg aaacggcgtt gctcgtcacg acgacggaag gggtttcggc caccagggca
    12121 acgggggcca ggtccttgac cgggtcgtag gtcatcttct tgtacagcag cgcgtcggtt
    12181 tccagcgggc ccgacggcgc cagcagcaag gtcgaaccgt cgggcgtgga tttcgcgacg
    12241 aagtccgcgc ctatcatgcc acccgcgccc ggcttgttct ccacgatcac gggctgcccg
    12301 gtgctctggc cgacctggtc gaagatcaag cgggcgatga gatcgggcgt cgatcccgcg
    12361 gcatagggca cgatgaccag tatccgtttc gtcggccaaa cgggggctgc ccgcaaggcc
    12421 ccgcacaggc ccaggcacag cagcaacacg gtgatgcagc gtttcatgtc tctctccttt
    12481 ctttgttttt gttccggcct tgcgccgggc gttatcgggt actgtcctgc agagcccgcc
    12541 attgttcggc cgtccggccc aggttgccga agcgcgcgaa ggcccagcgc atgacctcgt
    12601 cctcctcggt ccagggcgcc cggtcaatct gcagccacaa tcggtacagg aaccggcgcc
    12661 gcgcgggatc cggatcgtct tcgtaggccg tcctgccgtg gaccacggta tggttgttca
    12721 gcagctggat gtcgccgggc cggaattcca tgtgcatcgc catgtcttcc cggcgcgcga
    12781 tcgcatcgaa gcaatccagc gcggcgcgct cgtcgtcgtc gagcgcctgc cccagcttct
    12841 gggccgcgcc ctggatgtag gagcgggcat agcggcaact gacgcgcccg ccatggtgca
    12901 cgaacacggg gatgcgcggc gtcacgggcg cttcggccgc ccagccctcg cccttgcggt
    12961 cgtaggcgaa gccgcgcagc agcacgggca ggtgctcggg atgtccagcg cggatctcat
    13021 tgaatatcgc aggcgtgctg acgatcgtgc tgatgccgcc ggagcgggcc tggcgcaggc
    13081 aaagcagcgc gacgacatcg gtgggatcgc agtggtagtt caggtccgcc cggctctggt
    13141 agccgcggac gttgtattgc gaggccgcgc cgctgtagcc gaagtccacg cccatgtccg
    13201 tgaccgggca caggaagtcg gccttggcgt tctggctcag gccgacgccg aacagcagcc
    13261 ctatccccca gtaggcgagc cgcgcctctt cgtcggacag gcgctgcacc ggcacgccgc
    13321 ggatcagcgc gaagccgcgc ccggccagtt agccggtcac gcggcttgcc agggaagcca
    13381 ggccggcaag cgcctgtccg gcctcttcgc gggtcagtga atcgaacgcc cggccgcgcc
    13441 agcgctgcgc ggcacgcgcc acctcttcgg cctgatcctg ggaaagctca tggcaccagg
    13501 agtgatcctg gacaaaatcc gcagggcgcc aggcggacgg cccggcgacg ggtggcagcg
    13561 cctgctgcat tgcatcacgc tcttgcacga tgtctccttg ttttcatgtt ttatgcatgc
    13621 agttttccag cttgacttcc ttaagtaaaa ggaaaaaata tcggtctcta ttaaccaaaa
    13681 gttaatttca tgaatcttcg ctacctggaa gtgttccggc tggtcatgca gaccggcacc
    13741 atcaagggcg ccgcggccct gatgcacgtt tccgaggccg cggtcagcaa attgctgggc
    13801 accgccgaac gccgcatggg gctggccctg ttcgagcgcg tcaaggggcg gctcatcccc
    13861 acccccgaag cgcgtcgcct gtacgaagaa gtcgagctgc tatggacgcg cgtggagcgc
    13921 atcgagaacc tgacggcgtc gctggccaac cccgacggcg ggtcgctcac ggtggccatc
    13981 tcgcccagcc tgggaacgac cgtcgcgccg gccgccgcga ccgcgctgct ggcacgcctg
    14041 ccccgcgcca ccatcaacat cgagctcctg atccctcaat tgctcgtgca ggcgctggtc
    14101 gacggcatcg ccgaactggg cgtctcgctg agcccgcagg aacaccccag cctgcaggtc
    14161 ttgcagcgtt acccgtgcgg gctggcctgc gtcatgccgc gcgggcaccc cttggcgaag
    14221 aaacgcgtga tcgggccagc cgatctgcga gggcacgcgg tggtgtcgtt tccccaggcg
    14281 cttgcatacg gaataaccga ggcccagcta tacggcagcc acgcggacga aatcaacgtg
    14341 cggctgaacg tgcgttcggg acaaaccgcc tgctggttca gcctggctgg agccggcgtg
    14401 gcgatcgtcg atgccgcgac ggtggccggc caagcctttc ccgacctggc ggtcaggccc
    14461 tatcgcagtc aggccaggct ggccttgcac gtgctgcgcc atcgcaaccg gccgctgtcg
    14521 cgggccggga tcattttctg cgaaacgctg gacgagacgt ggaaacggct tgggaaggaa
    14581 gggtaaagag cattcgagct ttggggatta ttcgacagtc ggcctggctt caacggccgt
    14641 taagcgccgc ttccatttca agccgcgcgt cttcgatgtc gtcgagcagc gcgtcgagcg
    14701 caacaggggt ggtgtcgatc tcgacgtgcc cggtcaatgc cacgccgacg tgcagtccgc
    14761 cgcgctcgaa cagcccccat atctctttgc cgtaatgggt cgtgagaagc tggggcgcgg
    14821 cctggccgaa aaagaccttg aggttgtcca cgtcgcgttc gagcatcgcg cgggcctcga
    14881 ggttgccggc ggcattcacg gcctggggca ggtcgatgat gacggggccg tcggcggcca
    14941 gcagcgtgtt gtattccgaa agatcgccgt ggatcagccc ggcgcacagc atgcgcacca
    15001 cctgatcgag cagccgcgca tgcagatcca gggcctgggt ctcggtcatg gcgacatcgc
    15061 ccagccgggg cgcgacgttg ccggcctcgt ccgtgaccag ttccatcagc agcacgccgt
    15121 cggtgcagat gtagggctgc ggcacgcgca cgccggcgtt ggccagcagg aacagggcat
    15181 cgacctcggc cgtctgccat agtttttcct gcacctggcg gccgtaacgg gtgccttttt
    15241 ccagggcgcg cgcttcgcgg ctgttcttga ccttgcggcc gtcgagatac gacgcggcct
    15301 gccggaagct gcgctgcttc acgtctttgt agaccttggc gcaccgcacg gactcgccgc
    15361 agcgcaccgt gtataccgtg gcttccttgc cgctcatcag ttggccgagc acttcgtcga
    15421 tgagaccttc ctggagcagc ggcatgagcc gcttaggggt cttcacgcgg tctccggcaa
    15481 aggcgggacc gcgcacgaac ggcgagggtg gcagggcaaa taggtcatgc gcgattatag
    15541 aggcccggcg ccatgccggc ccgggtcgct gggcaacgcc gtgtcagccc ccgtttccga
    15601 aaccgccggc ggcccattcc gatgcgcgtt gtacgcccag cttgtacgtc ggtaacacac
    15661 ggatttgggc cagttgttca ccggattcat cgtaggcgct cagcaaagca tacggatcgt
    15721 cttcgtcggg aatgatgtcc agcttgatgg tcaggcgacc gccggcggca tcgatcttca
    15781 cgctcttgtg cagcgtggca tgcaactgct gttcatgccg gcgcaagacc tcgttggcgg
    15841 tcttgaccag cgtgaagaaa gcaggggcgt cgagcggctt ggggttcttc ttgtcgcggc
    15901 ccatggtcca gggcccgacc agggccggtt cggcttcgcc atcctgcgtc atctccacgg
    15961 cccagccgtc atcgtcctcg ttcttgatga ctcgggcagt ccagccgtcg ttgcgccaca
    16021 gcctgggctc ctgcgtcttg cggtcttcgg gtatgtccgt gtccgggatc tggtcgtcat
    16081 tcactgctgc aatactcctg gttccgtcag gccatctgcc tgaggatggc atccaccagc
    16141 cctggaaacc ggctgttcag ttcggcccgc cgcaacgtat tctggtgcac ggtcccggca
    16201 acgctagtct gcaccaagcc cgcctcgcgc agtatgcgga agtgatggga catgctcgac
    16261 ttggggcggc cgccgtcaag ttcgccgcag gtggcctcct ccacatgcgc gaggcgttgg
    16321 acgatctcca gtcgcaccgg gtcgctcaag gcatgaaaaa tacgctcgag gacaaagttt
    16381 gcgggatcag gatggttgta agtacgcata tagcggattg taatgcgttc ttcgatagta
    16441 cgataattat cgaactacgg tactattggc ggccaagctt ttctacggaa cccgatcgat
    16501 gtcctcgtta ttcaccccct acaagctcaa agacgtatcc ctgcgcaacc gcatcgccgt
    16561 tccgcccatg tgccagtact ccgccgatga cggcctgatc aacgactggc accgcgtgca
    16621 tttggccgga ctcgcgcgcg gcggcgcggg gctcgtcatc gtcgaggcaa cggccgtttc
    16681 gccggaaggc cgcatcaccc ccggatgcgc cggcctgtgg aacgacgagc aggcgcaggc
    16741 tttcgtgccg gcggtcgcat cgatcaaggc agccggcgcc gtgcccggca tccagatcgg
    16801 ccatgccggc cgcaaggcca gcgccaaccg tccgtgggaa ggcgacgacc atatcgccgc
    16861 ggacgatccg cgcggctggc cgaccattgc gccgtcggcg attgcattcg gcgagggtct
    16921 ctccaagatg cccagcgcga tgagcctcga cgatatcgcg cgcgtgcgcc aggatttcgt
    16981 tgccgccgcc aaacgcgcgc gcgacgccgg cttcgaatgg ctggaactgc actttgccca
    17041 tggctatctg gggcagagct tcttttcgcc ccactcgaac cagcgcaatg acgcttacgg
    17101 cggcagcctg gaaaatcgca gccgcttctt gctggagacc ttggctgccg tgcgcaaagt
    17161 gtggcccgag catttgccgc tgacggcccg cttcggcgtg atcgagttcg acggacgcga
    17221 tgaagaaacc ttggcggaat ccatcgaact ggccaggaac ttcaagcgcg aaggcctgga
    17281 tatgctgagc gtcagcatgg gtttttccac gccgacggca aacattccgt gggcgccggc
    17341 gctgctggcg ccgatcgccg aacgtgtacg ccgggaagcg gacattcccg tttcgtccgc
    17401 ctggggcatc gacacgcccg aattggcgga tggcgccgtc aagcatggcc agctcgatct
    17461 cgtcatggtg ggccgcgcgc atctggccga cccgcactgg ccgtattacg ccgcgcgcaa
    17521 gcttggcatc gaacgcccgt cgtggacgct gccggcgcct tacgcgcatt ggcttgaacg
    17581 ctatgccgtc aactaagcca cccctggggg catgacgccg ccggcccggg tattccgggc
    17641 cggcatccgg cttgatctca caccatttcg atggccatgg ccacggcctc gccgccgccg
    17701 atgcacagcg tggccacgcc cttcttgccc ccggt
//
LOCUS       DASWPO010000237        10110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2556522, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000237 DASWPO010000000
VERSION     DASWPO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556522"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..2328)
                     /locus_tag="VGC69_12480"
     CDS             complement(76..2328)
                     /locus_tag="VGC69_12480"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY0296140.1"
                     /translation="MSAPPVPALAAPFDDAWLQAAGEGLDEDGVRLLARAAAWVAPRF
                     HDHQALTGEPLIGHAAGAARLLAGLHTDAATRAAALLASVPPEAMPPASSRNDPIAAE
                     FGAETAKLLAGSRALLRLGVMARQASDSAADTGDQKEMQRKMLLAMAADLRIVLMRLA
                     SRLQTLRWHAETKVPCPPALARETLDLYTPLANRLGIWQLKWELEDLAFRFLEPDRYK
                     QIARQLEEKRVEREAFITDAVRRLGEALARAGIAAEVYGRPKHIYSIWNKMRVKSVDF
                     SQLLDLRALRVIVGDVRACYAALALVHEMWTPMTEAFDDYISRPKPNGYRSLHTVVID
                     ADGRPFEVQIRTREMHQFAEYGMAAHWRYKEAGAKGGQVSASSDYDRQLSWMRQLLAW
                     NSELETRGQGDAAAADAVAANAAAAQMGDGRIYVLTPQARVIELPEGATPVDFAYHLH
                     TDLGHRCRGARIDGQMVPLNTRLATGQTVEVIAAKSGGPSRDWLNPQLGYLASPRARS
                     KVRLWFNAIELAQRITQGQALVEKELQRLGKTAVNLEQLAQALGFARADDLYVAVAKE
                     EFSLRHIDSAFQQPAPASEAPPPVPKFTRSADSAETSGKSGVLVVGVGSLLTQLARCC
                     RPAPPDLIRGFVTRGRGVSIHRADCASYRTLAAREPERVIDVTWGNTADTFYPVDVSV
                     RAHDRPGLLRDLSEVFARQRLNVVGVNTQSKQSLAHMVFTVEVRDGEALHRALDALAA
                     VADVTSAARR"
     gene            complement(2425..2775)
                     /locus_tag="VGC69_12485"
     CDS             complement(2425..2775)
                     /locus_tag="VGC69_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0296141.1"
                     /translation="MSSIKRINVGKRLSDVAVYNGVAYVAGQIPDDATLDITGQTKQV
                     LATIDKLLAEAGTDKSRILMAQVFVANMAEFAGMNAAWDAWVADGAAPPRATIEARLA
                     NPYLKVEIVVTAAV"
     gene            complement(2830..4005)
                     /gene="pncB"
                     /locus_tag="VGC69_12490"
     CDS             complement(2830..4005)
                     /gene="pncB"
                     /locus_tag="VGC69_12490"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY0296142.1"
                     /translation="MIITSLLDTDLYKFSMMQVVLHQFPAAQVEYRFKCRTPGVDLRP
                     YLDEIRAEVHHLCQLRFTDEELEHLRSLRFLSSDFIDFLGLFHMPERCIDIAAGERPG
                     EIAIGIKGPWLHTILFEIPVLAIVNEVYFRNTQPRPDLEEGRRRLQAKMHLVLDDPAL
                     AGFRVADYGTRRRFSKVWHEEVVTTLKGQMTPHFVGTSNVLLAKRHGVLPLGTMGHEY
                     LQACQALGPRLRDSQVYALEMWAKEYRGDLGIALSDVYGMGAFLRDFDMYFCKLFDGA
                     RHDSGDPFIWGERMIEHYRNNRTDPATKTLVFSDSLNFPKAIELFRQFSGRSKVSFGI
                     GTNLTNDLGYEPLQIVIKMVRCNGQPVAKVSDSPEKTMCDDPAYLAYLRQVFDLPPP"
     gene            4235..4558
                     /gene="fdxA"
                     /locus_tag="VGC69_12495"
     CDS             4235..4558
                     /gene="fdxA"
                     /locus_tag="VGC69_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY0296143.1"
                     /translation="MTHVVTENCIKCKYTDCVDVCPVDCFREGPNFLVIDPDECIDCA
                     VCIPECPANAIYAEEDVPQDQLKFIQINAELTSEFQSISRAKKPLPDADQWNGVPDKL
                     QHLEK"
     gene            4559..5338
                     /locus_tag="VGC69_12500"
     CDS             4559..5338
                     /locus_tag="VGC69_12500"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY0296144.1"
                     /translation="MAQAKYTRQTVTHVQPWVPGKLFSLRVSRDPAFAFQPGQFARLG
                     LPVIAPAGAGPDGEPTLWRAYSMVSAPREAELEFYSIVVPEGPFSPRLAQLRPGDALY
                     VDQTAFGFLTLERFPDGDDLWLLATGTGLSAYLSILRDPAARQRFARVVLVHGVRTAD
                     ELAYRDELQDLGRQCAGFTYLPVATREALPGMPQARLTTLITDGGLEKLAGLALDPAR
                     SRIMLCGNPDMLSDARKLLGERGFAPGRRGVPGNLAVENYW"
     gene            5495..6715
                     /gene="phaZ"
                     /locus_tag="VGC69_12505"
     CDS             5495..6715
                     /gene="phaZ"
                     /locus_tag="VGC69_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY0296145.1"
                     /translation="MLYQLHEMQRAFLRPLAEFTDAGAQWFSNPANPLSYMPISRQMV
                     AGLQLVHRIGKDYHKPAWDIDSVDIGGKPVAVRESCAVARPFCHLLHFERELPARRAR
                     DPRVLLVAPMSGHHATLLRDTVRALLPSHDVYVTDWIDARMVPLSAGPFHLDDYVRTI
                     QEFVRHLGPDVHVISVCQPTVPVLAAISLMASAGDIQPRSMVMMGGPIDPRQSPTQVN
                     NLATTKPYEWFESQVIHAVPGNYRGAGRLVYPGFLQHAGFIAMNPDRHLKSHYDFYLD
                     LLRGDDDDAESHRRFYNEYNAVLDMPAEFYLDTIRIVFQEFRLPNGTWEIDGQLVKPA
                     DIRKTALLAIEGELDDISGVGQTKAAIALCRGIPAARKQYFLAKGAGHYGIFSGRRWR
                     DMVCPKIAEFIRQS"
     gene            6712..7347
                     /gene="rsxB"
                     /locus_tag="VGC69_12510"
     CDS             6712..7347
                     /gene="rsxB"
                     /locus_tag="VGC69_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEY0296146.1"
                     /translation="MTDLATRIDALLPQTQCTKCGYDGCRPYAQAIADGRAPIDRCPP
                     GGEAGIAALANLLAVPALPLDTTRGRHGPLLAAVIDEAHCIGCTLCIQACPVDAIVGA
                     NKRMHTVLADLCTGCDLCVPPCPVDCISMVPANRAWTPADAQAARQRHQARGARLARL
                     AADNTRLMADAPALDQPAAAAAPTPHDAERKRSAVESALARARARRAAKQP"
     gene            7379..8014
                     /gene="nth"
                     /locus_tag="VGC69_12515"
     CDS             7379..8014
                     /gene="nth"
                     /locus_tag="VGC69_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0296147.1"
                     /translation="MNATKRREIFARLQAANPHPRSELEYETPFQLLIAVLLSAQATD
                     KSVNLATRKFFPKYGTPEKLAALGEARLEEFIKTIGLFRTKAKNAIATSRLILERHGG
                     EVPHSREALEALPGVGRKTANVVLNIAFGEPTIAVDTHIFRVANRTGLAPGKDVLAVE
                     LKLEKVVPRDYAQHAHHWLILHGRYVCVARTPKCPQCGIADLCDFKSKTKA"
     gene            8188..8961
                     /locus_tag="VGC69_12520"
     CDS             8188..8961
                     /locus_tag="VGC69_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296148.1"
                     /translation="MSDAILETKKLTKEFRGFVAVNGVDLRIERGEIHALIGPNGAGK
                     TTCFNLLTKFLSPTSGSIFFNGVDITRERPAQIARRGVIRSFQISAVFPHLTVLENVR
                     IGLQRKTGLSYHFWQSESPLQRLDARARELLDEVDLGGFAGEITLNLPYGRKRALEIA
                     TTLAMEPELMLLDEPTQGMGHEDVDRVTQLIKRVSRGRTILMVEHNMAVISSIADTIT
                     VLARGAVLAQGNYAEVSRHPAVMQAYMGTTDGQLQGAHP"
     gene            8958..9749
                     /locus_tag="VGC69_12525"
     CDS             8958..9749
                     /locus_tag="VGC69_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296149.1"
                     /translation="MSTPALQLSALHAWYGESHILHGVDLEVGQGEVVTLLGRNGAGR
                     TTTLRAILGLTGSRKGSVRIHGTESIALPTYRVAHLGVGYCPEERGIFASLSCEENLL
                     LPPPLQSAASGGRLPPGRPKATDASLEGSGPQAPLGGAIPLAEIYEMFPNLHERRHSP
                     GTRLSGGEQQMLAVARILRTGANLLLLDEISEGLAPVIVQALARMIVALKARGYTIVM
                     VEQNFHFAAPLADRFYVMEHGQIVEHFAASQLAEKQDTLNELLGV"
     gene            9809..>10110
                     /locus_tag="VGC69_12530"
     CDS             9809..>10110
                     /locus_tag="VGC69_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296150.1"
                     /translation="MKLRNLTAALAAAGLGLAGLSAQAAGISDDVVRIGLITDMSGVY
                     ADIDGKAGVTAIQMAVDDFGGTVDGKKIEVLSADHQNKADVAASRAREWFDQQKVD"
BASE COUNT         1728 a         3581 c         3238 g         1563 t
ORIGIN      
        1 ctggctactt ccggctgaac aagggcgaat tcgcgggttt ctcccaggcc cgcgggctcg
       61 ccagcgcgca ttgcgtcagc gccgcgcggc cgaggtgacg tccgcaacgg cggccagggc
      121 gtccagcgcg cggtgcaggg cctcgccgtc gcgcacctcc acggtgaaca ccatgtgcgc
      181 cagcgactgc ttgctctgcg tgttcacccc caccacgttc aggcgctgcc gggcgaagac
      241 ttcggacagg tcgcgcaaga ggcccggccg gtcgtgggcg cgcacgctca cgtccaccgg
      301 gtagaaggta tcggcggtat tgccccaggt cacgtcgatg acccgctcgg gctcgcgggc
      361 ggccagggtc cggtagctgg cgcagtcggc ccggtggatg gacaccccgc gcccgcgcgt
      421 gacgaagccg cggatcaggt cgggcggcgc cggccggcag cagcgcgcca gctgggtcag
      481 cagcgacccc acccccacca ccagcactcc gctcttgccg ctggtctcgg cgctgtcggc
      541 gctgcgggtg aacttcggca cgggcggcgg cgcctccgag gccggcgcgg gctgctggaa
      601 ggcgctgtcg atgtgccgca ggctgaactc ttccttggcg accgccacgt acaggtcgtc
      661 ggcgcgggca aagcccagcg cctgggccag ttgctcgagg ttgaccgccg tcttgcccag
      721 gcgctgcagc tctttttcca ccagggcctg gccctgggtg atgcgctgcg ccagctcgat
      781 ggcgttgaac cacaggcgca ccttgctgcg cgcgcgcggg ctggccaggt agcccagttg
      841 cggattgagc cagtcgcgcg acgggccgcc cgacttggcg gcgatgacct ccaccgtctg
      901 gcccgtggcc aggcgcgtgt tcaggggcac catctggccg tcgatccgcg cgccgcggca
      961 gcggtggccc agatcggtgt gcaggtggta ggcgaagtcg accggcgtgg cgccctcggg
     1021 cagctcgatc acgcgcgcct gcggggtaag cacatagatg cgcccgtcgc ccatctgcgc
     1081 cgccgccgca ttggccgcca cggcatcggc cgccgcggca tcgccctgcc cgcgggtttc
     1141 cagctcgctg ttccaggcca gcagctggcg catccacgac agctgccggt cgtagtcgct
     1201 ggaagccgac acctggccgc ccttggcgcc cgcctctttg tagcgccagt gcgccgccat
     1261 cccgtattcg gcgaactggt gcatctcgcg cgtgcggatc tgcacctcga agggccggcc
     1321 gtcggcatcg atcaccaccg tgtgcagcga gcggtagccg ttgggcttgg gccgcgaaat
     1381 atagtcgtcg aaggcctcgg tcatcggcgt ccacatttcg tgcaccaggg ccagggcggc
     1441 ataacaggcg cgcacgtcgc cgacgatcac gcgcagggcg cgcaagtcga gcagctggga
     1501 gaagtccacg ctcttgacgc gcatcttgtt ccagatgctg tagatatgct tgggccggcc
     1561 atagacctcg gcggcgatgc cggctcgcgc cagggcctcg cccagccgcc tgacggcatc
     1621 ggtaatgaag gcttcgcgct cgacgcgctt ttcctcgagc tgtcgcgcga tctgcttgta
     1681 gcggtcgggt tcgaggaagc ggaaggccag gtcctcgagc tcccacttga gctgccagat
     1741 gcccagtcga ttggccagcg gcgtgtagag atccagcgtt tcgcgcgcca gggcgggcgg
     1801 acacggcacc ttggtttcgg cgtgccagcg cagggtctgc aggcgcgagg ccaggcgcat
     1861 cagcacgatg cgcaggtcgg ccgccatggc cagcagcatc ttgcgctgca tttccttctg
     1921 gtcgccggtg tcggccgcgc tgtcgctggc ctgccgggcc atgacgccca ggcgcagcaa
     1981 ggcgcgcgaa cccgccagca gcttggcggt ttcggcgccg aactcggcgg cgatgggatc
     2041 gttgcgcgac gaggcgggcg gcatggcttc gggcggcacc gaggccagca gggccgccgc
     2101 ccgggtggcc gcatcggtat gcaggccggc cagcagcctg gcggcgccgg cagcatggcc
     2161 gatcagcggc tcgcccgtca gcgcctggtg atcgtggaag cgcggcgcga cccaggccgc
     2221 ggcacgcgcc agcaggcgca cgccgtcttc gtccaggccc tccccggcag cctgcagcca
     2281 ggcgtcgtcg aacggcgcgg caagggcggg aacaggcggg gcggacatcg gcaagccact
     2341 tggacagaaa tgcggtattc acactctacc gcaaaaaaac agggccgccc cgaggcggcc
     2401 ctgcgctgtc gcgatgccgc ggaatcagac cgcggcggtg accacgatct cgaccttgag
     2461 gtaggggttg gccaggcggg cctcgatggt ggcgcgcggc ggcgcagcgc cgtcggccac
     2521 ccaggcgtcc caggcggcgt tcatgccggc gaattcggcc atattggcca cgaagacctg
     2581 cgccatcagg atgcgcgact tgtcggtgcc ggcttcggcc agcagcttgt cgatggtggc
     2641 cagcacctgc ttggtctggc cggtgatgtc cagcgtggcg tcgtcgggaa tctggccggc
     2701 cacataggcc acgccgttgt acaccgcgac gtcggacagg cgcttgccca cattgatgcg
     2761 tttgatgctg ctcatggaat ccccttatga cgaaaaaatc ggtgggttgc ggaaaaaatc
     2821 gccctgcgct cagggcggcg ggaggtcgaa aacctggcgc aggtaggcca ggtaggccgg
     2881 atcgtcgcac atggtctttt ccggcgaatc ggacaccttg gccaccggct ggccattgca
     2941 gcggaccatc ttgatgacga tctgcagcgg ctcgtagccg aggtcgttgg tgaggttggt
     3001 gccgatgccg aacgatacct tgctgcgccc cgagaactgc ctgaacagct cgatggcctt
     3061 gggaaagttc agcgaatcgg aaaacaccag ggtcttggtg gccgggtcgg tgcggttgtt
     3121 gcggtagtgc tcgatcatgc gctcgcccca gatgaagggg tcacccgaat cgtggcgcgc
     3181 gccgtcgaac agcttgcaga agtacatgtc gaaatcgcgc aggaaggcgc ccatgccgta
     3241 gacgtcggac agggcgatgc ccaggtcgcc ccggtactcc ttggcccaca tttccaacgc
     3301 atagacctgc gagtcgcgca ggcgcgggcc cagcgcctgg caggcctgca ggtattcatg
     3361 ccccatggtg cccagcggca gcacgccgtg gcgcttggcc aggagcacat tgctggtgcc
     3421 gacgaaatgc ggggtcatct ggcctttcag cgtggtcacg acctcttcgt gccacacctt
     3481 ggaaaaacgc cggcgcgtgc cgtagtcggc cacacgaaag ccggccagcg cgggatcgtc
     3541 gagcaccagg tgcatcttgg cctgcaggcg cctgcgcccc tcttccaggt cgggccgggg
     3601 ctgggtattg cggaaataga cctcgttgac gatggccagc accgggatct cgaacaggat
     3661 ggtgtgcagc caggggccct tgatgccgat cgcgatctcg ccgggacgct cgcccgcggc
     3721 aatgtcgatg cagcgctcgg gcatgtggaa caggcccagg aaatcgatga aatcgctgga
     3781 gagaaagcgc aggctgcgca ggtgttccag ctcttcgtcg gtaaagcgca gctggcacag
     3841 gtgatgcacc tcggcccgga tctcgtccag atagggccgc aggtcgacgc ccggcgtgcg
     3901 gcacttgaac cggtactcca cctgggcagc cgggaactgg tgcagcacga cctgcatcat
     3961 gctgaatttg tagagatcgg tgtcgagcag cgaggtgatg atcatggaag acgaaagcgc
     4021 ctgctagatt tgcgcacaca ttaacacggc ggtgcatgaa tcccggctct gagccgaggc
     4081 cgcgccggag gaatccgagg cgcaggcgct acgagcgccg agcatcggaa ttccgcaggg
     4141 gcggtctcgg cggctcaaga acccatgtgc cgccgccatt aggtaaaatc cctcagatca
     4201 actatagcca tagccctgcc cggagcccgc aagcatgacc cacgtcgtca ccgaaaactg
     4261 tatcaagtgc aagtacaccg attgcgtgga cgtctgcccg gttgactgct tccgcgaggg
     4321 cccgaacttc ctggtcatcg atcccgacga gtgcatcgac tgcgccgtct gcatccccga
     4381 atgcccggcc aacgccatct acgccgagga agacgtgccg caggaccagt tgaagttcat
     4441 ccagatcaac gccgagctga ccagtgaatt ccagagcatc agccgggcca agaagcccct
     4501 gcccgatgcc gaccagtgga acggcgttcc cgacaagctg cagcacctcg aaaagtaagt
     4561 ggctcaagcc aaatacacgc gccagaccgt cacccacgtc caaccctggg tgccgggcaa
     4621 gctgttctcc ctgcgcgtca gccgcgaccc ggccttcgcc ttccagcccg gccagttcgc
     4681 ccggctgggc ctgccggtca ttgcccccgc cggcgccggc ccggacggcg agcccacgct
     4741 gtggcgcgcc tactccatgg tgtcggcccc gcgcgaggcc gagctcgagt tctattccat
     4801 cgtcgtgccc gaaggcccct tcagcccccg gctggcgcaa ctgcgcccgg gcgatgccct
     4861 gtacgtcgac cagacggcct tcggcttcct gacgctggaa cgctttcccg acggagacga
     4921 cctgtggctg ctggccaccg gcacggggct gtcggcctac ctgtccatcc tgcgcgatcc
     4981 agcggcgcgg cagcgtttcg cccgcgtcgt gctggtgcac ggcgtgcgca cggccgacga
     5041 gctggcctat cgcgacgaac tgcaggacct gggccggcaa tgcgcgggct tcacctacct
     5101 gcccgtcgcc acccgcgaag ccctgcccgg catgccccag gcccgcctga ccacgctgat
     5161 caccgacggc gggctggaaa agctggccgg cctggcgctg gacccggccc gctcgcgcat
     5221 catgctctgc ggcaaccccg acatgttgtc cgatgcgcgc aagctgctgg gcgaacgcgg
     5281 cttcgccccg ggccgccgcg gcgtgccggg caacctggcc gtcgagaact actggtagcc
     5341 ggacaagcca ttggtcgctt ttttgcaacg caacataaac caaacccgtg ttgcaagtgc
     5401 ttgttctgac atattttctt aatatcgggg ttgaccgctt tgtcaccatg ccgggcgcgg
     5461 gacacgcccg accaaaaaca acaggaatac acgcatgctg tatcaattgc atgaaatgca
     5521 gcgggcattt ctccgtccgc tggccgaatt caccgatgcc ggtgcgcagt ggttctccaa
     5581 tcccgccaac cccctgtcgt acatgcccat ctcgcgccag atggtggccg ggctgcagct
     5641 ggtgcaccgc atcggcaagg actaccacaa gcccgcctgg gacatagaca gcgtcgacat
     5701 cggcggcaag cccgtggccg tgcgggaaag ctgcgcggtg gcccgcccct tctgccacct
     5761 gctgcacttc gagcgcgaac tgccggcccg gcgcgcccgc gatccgcgcg tgctgctggt
     5821 cgcccccatg tcgggccacc acgccacgct gctgcgcgac accgtccggg cgctcctgcc
     5881 cagccatgac gtctacgtca ccgactggat cgacgcccgc atggtgccgc tctcggccgg
     5941 ccccttccac ctggacgact acgtgcgcac catccaggag ttcgtgcgcc acctggggcc
     6001 cgacgtgcat gtgatctcgg tctgccagcc cacggtgccg gtgctggcgg ccatttcgct
     6061 catggccagc gcgggcgaca tccagccgcg cagcatggtg atgatgggcg gccccatcga
     6121 cccgcgccaa tcgcccaccc aggtcaacaa cctggccacc accaagccct acgagtggtt
     6181 cgagagccag gtcatccacg ccgtgcccgg caactaccgc ggcgcgggcc ggctcgtcta
     6241 ccccggcttc ctgcagcacg cgggcttcat cgccatgaac cccgaccgcc acctgaagtc
     6301 gcattacgac ttctacctgg acctgctgcg cggcgacgac gacgatgccg agtcgcaccg
     6361 ccgcttctac aacgagtaca acgccgtgct ggacatgccg gccgagttct acctggacac
     6421 cattcgcatc gtgttccagg agttccgcct gcccaacggc acctgggaaa tcgacggcca
     6481 actggtcaag cccgccgaca tccgcaagac cgcgctgctg gcgatcgaag gcgagctgga
     6541 cgatatttcc ggcgtgggcc agaccaaggc ggccatcgcc ctgtgccgcg gcatcccggc
     6601 cgcgcgcaag cagtacttcc tggccaaggg cgccggccac tacggcattt tctcgggccg
     6661 gcgctggcgt gacatggtct gccccaagat cgcggaattc atccggcagt cgtgaccgac
     6721 ctcgcaaccc gcatcgacgc cctgctgccg cagacgcaat gcacgaaatg cggctatgac
     6781 ggctgccgcc cctatgccca ggccatcgcc gacggccggg cgccgatcga ccgctgcccg
     6841 ccgggcggcg aggcaggcat cgcggcattg gccaacctgc tggccgtgcc cgccctgccg
     6901 ctggacacca cccgcggccg gcacggcccg ctgctggccg ccgtgatcga cgaggcgcac
     6961 tgcatcggct gcaccctgtg catccaggcc tgcccggtcg acgccatcgt cggcgccaac
     7021 aagcgcatgc acacggtgct ggcggacctt tgcaccggct gcgacctgtg cgtgccgccc
     7081 tgcccggtcg actgcatcag catggtgccg gccaaccgcg cctggacgcc cgccgacgcc
     7141 caggcggcgc gccagcgcca ccaggcccgc ggcgcgcgcc tggcacgcct ggcggccgac
     7201 aacacccggc tgatggccga cgcgcccgca ctcgaccagc ccgccgccgc ggccgccccc
     7261 accccgcacg acgccgaacg caagcgcagc gccgtcgaat ccgccctggc ccgcgcccgc
     7321 gcgcggcggg ccgccaaaca gccatgaatc ccgccaagca cgcgcttgaa atccaccgat
     7381 gaatgcgacc aagcgccgtg aaatcttcgc ccgcctgcag gcggccaatc cgcacccccg
     7441 aagcgagctc gaatacgaga cgccgttcca gctgctgatc gccgtgctgc tgtcggccca
     7501 ggccaccgac aagtcggtca acctggccac gcgcaagttc tttcccaagt acggcacgcc
     7561 cgaaaaactc gcggcgctgg gcgaggccag gctggaagaa ttcatcaaga ccatcggcct
     7621 gttccgcacc aaggccaaga acgccatcgc caccagccgg ctgatcctgg agcgccacgg
     7681 cggcgaagtg ccgcacagcc gcgaggccct ggaagccctg cccggcgtcg gccgcaagac
     7741 ggccaacgtg gtgctcaaca tcgccttcgg cgaacccacc atcgcggtgg acacgcatat
     7801 cttccgcgtc gccaaccgca ccggactggc gcccggcaag gacgtgctgg cagtcgagct
     7861 caagctggaa aaagtggtgc cgcgcgacta tgcgcagcac gcgcatcact ggctcatcct
     7921 gcatggccgc tatgtctgcg tggcgcgcac gcccaagtgc ccccagtgcg gcatcgccga
     7981 cctgtgcgac ttcaagtcca agaccaaagc ctgacccgag acttacgtct cgacttccgt
     8041 gagtcgggcc atgtgacacg acacaccatt gcaagacggc cactgggcgg caaagcctgg
     8101 taaaacctat gtaaaaccct catggcaatt tggagagggc gtccgcatac tcgccgcagt
     8161 tttataaaaa gcgaaggaca catcaccatg agcgatgcga tcctggagac aaaaaagctt
     8221 accaaggaat tccgcggttt cgtcgcggtc aatggtgtcg atctgcggat cgagcgcggg
     8281 gaaatccatg ccctcatcgg tcccaacggt gcgggcaaga ccacctgctt caatctgctc
     8341 accaaattcc tgtcgcccac ctccggctcg atcttcttca acggcgtcga catcacccgc
     8401 gagcggcccg cgcagatcgc gcgccgcggc gtgatccgat cgttccagat ctcggcggtt
     8461 ttcccgcatc tcacggtgct ggaaaacgtg cgcatcggcc tgcaacgcaa gaccggcctt
     8521 tcctatcact tctggcaaag cgaaagcccg ctgcagcgcc tggacgcgcg cgcccgcgag
     8581 ctgctcgacg aggtcgacct cggcggcttc gccggcgaga tcaccctcaa cctgccctac
     8641 ggccgcaagc gcgccctgga aatcgccacc accctggcca tggagcccga gctcatgctg
     8701 ctcgacgaac ccacgcaggg catgggccac gaagacgtcg accgcgtcac ccagctcatc
     8761 aagcgggtga gccgcggccg caccatcctc atggtcgaac acaatatggc cgtgatctcg
     8821 tccatcgccg acaccatcac cgtgctggcg cgcggcgccg tgctggcgca gggcaactat
     8881 gccgaggtct cccgccaccc cgccgtcatg caggcctata tgggcacgac cgacggccaa
     8941 ctgcaggggg cccacccatg agcacgcccg cattgcagct ctcggcactg cacgcctggt
     9001 acggcgaatc gcacatcctg cacggcgtcg acctcgaagt cggccaaggc gaggtcgtga
     9061 cgctgctcgg ccgcaatggc gccggccgca ccaccacgct gcgcgccatc ctcggcctga
     9121 ccggctcgcg caagggttcg gtgcgcatcc acggcacgga atcgatcgcg ctgcccacct
     9181 atcgtgtcgc ccacctgggc gtgggctact gcccggaaga gcgcggcatc ttcgccagcc
     9241 tgtcctgcga agaaaacctg ctgctgcccc cgccgctgca aagcgccgcg tccggcggcc
     9301 ggttgccgcc gggccgcccc aaggcaaccg acgcctcctt ggagggcagc gggccgcagg
     9361 caccgcttgg gggcgccata ccgctggccg aaatctacga gatgttcccc aacctgcacg
     9421 agcggcgcca ctcgccgggc acgcgcctgt cgggcggcga gcagcagatg ctggccgtgg
     9481 cccgcatcct gcgcaccggc gccaacctgc tgctgctcga cgaaatctcc gaaggcctgg
     9541 cgccggtgat cgtgcaggcc ctggcccgca tgatcgtcgc gctcaaggcg cgcggctaca
     9601 ccatcgtcat ggtggagcag aacttccact tcgccgcgcc gctggccgac cgcttctacg
     9661 tcatggagca cggccagatc gtcgaacatt tcgccgcatc ccaactcgca gaaaagcagg
     9721 acacgctcaa cgaattgctg ggcgtttgat tcaagaagcc tgttatatcc gccggcgatc
     9781 ccggctcaaa aagcaacgag gagacaccat gaaactacgc aatctcaccg ccgcgctggc
     9841 cgccgcgggc ctgggccttg ccgggttgag cgcccaagcc gccgggattt ccgacgacgt
     9901 cgtgcgcatc ggcctcatca ccgatatgtc gggggtgtat gccgacatcg acggcaaggc
     9961 cggcgtcacc gccatccaga tggcggtcga cgatttcggc ggcacggtcg acggcaagaa
    10021 gatcgaagtg ctgtcggccg accaccagaa caaggccgat gtggccgcct cgcgcgcgcg
    10081 cgaatggttc gaccagcaaa aagtcgacat
//
LOCUS       DASWPO010000238         5254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2199972, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000238 DASWPO010000000
VERSION     DASWPO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199972"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2150
                     /locus_tag="VGC69_12535"
     CDS             <1..2150
                     /locus_tag="VGC69_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEY0296151.1"
                     /translation="CAWPDSKHTATFEFCENGVKAVAAEATGKRATPTLFAANTVAAL
                     MRQSDYDLEQHGRLTDPLAYDAQTDTYRPIAWDDAFKLIARHLNGLASPDDAAFYTSG
                     RASNEAAFLYQLFVRMFGTNNFPDCSNMCHEATSRGLPATVGIGKGTVTLDDFELADT
                     VLIFGQNPATNHPRMLGELRACARRGATIVSINPLRERGLERFASPQSPVEMLTLSST
                     KIASTFIQPKVGGDFALIKGVAKHVLELDDEAVAQGGERVLDLDFIARHTQGFEAFAG
                     DLRAESWQVLESESGVARRDIEGLARIYAAGKRVIATWGMGITQHKNSVATIRMLSNL
                     MMMRGNIGREGAGLCPVRGHSNVQGNRTVGIEEKPTAAFLDRLGKAFGFEPPRRHGYD
                     VVETIEAMLEGRIKVFISLGGNFAMATPDTPRTFDALRRCDLTVQIATKLNRSHLVHG
                     KDALILPTLGRTEIDLQDGVPQGITVEDSMSMVHVSYGMNPPASGQLKSETAIVAGMA
                     DATLGGGRADWLGMASDYARIRDAIEASIAGFDRYNERIAQPGGFHLRVASRERDWKT
                     DTGKANFMVLPVEEDTPIRRARALHGERLMVLMTTRSHDQYNTTIYAMDDRYRGVFGQ
                     RRVVFINPADLEMLGFKAGEWVDMSTVWDDGIERRADSFLLVEYDIPRGCIGAYYPET
                     NPLVPLASVADVCNTPTSKSIPVLLRRGMPLAA"
     gene            2162..3079
                     /locus_tag="VGC69_12540"
     CDS             2162..3079
                     /locus_tag="VGC69_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEY0296152.1"
                     /translation="MIIRPTQHWLRLLFVWHGSVLQSILPQLLLMTGIGVAAVLTHGQ
                     VFGEKVPLNATPFTLCGVALTIFMAFRNNASYARYVEARHLWGNLLIATRTLTSQALA
                     YIPPGAGFAPRPFTHELIGFVHALKHQLRKTDPAQALAAHLEPALAQQLARQHYRPIA
                     ILRDVRQRLSGLQRRAVITDNQLWMLDTQLVEMEKTVGGCERLASTPIPFAYGVLLHR
                     TVYAYCALLPFGLVDSTGVFTPFVSVFVAYTFLALEAIASEITDPFGTAPNHLALNAI
                     TCTIERSLLELSGETMPDEAKPARRYQLT"
     gene            complement(3121..3582)
                     /locus_tag="VGC69_12545"
     CDS             complement(3121..3582)
                     /locus_tag="VGC69_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0296153.1"
                     /translation="MTQTPAPGIYLEDLAVGAVFRSGTHTMSVAEITQFARQYDPQVF
                     HLDDARARETLFGGLAASGWHTAAVTMRLLVDGGMPIATGLIGAGTELSWPRPVRPGD
                     TLRVETTVLAITPSKSKPDRAIVLCQSNTLNQRGEIVQQSTAKLVVFRRPA"
     gene            complement(3626..4210)
                     /locus_tag="VGC69_12550"
     CDS             complement(3626..4210)
                     /locus_tag="VGC69_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0296154.1"
                     /translation="MPEKLSHKERTHARIVEAAASAMREHGWDGIGVAALMKRAGLTH
                     GGFYAHFASRDELVEEAVDCMFKDTGAMLERCLHGKDGASGLAALIDFYLSDRLRKSV
                     DRGCPLPGLTGEASRMPAAARARFDRGVRHFRQAITAALTDMGRDDAQSLASSVLAEM
                     IGAMSLARATPEEKEGRDILHASRERLKERLGLA"
     gene            complement(4214..>5254)
                     /locus_tag="VGC69_12555"
     CDS             complement(4214..>5254)
                     /locus_tag="VGC69_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0296155.1"
                     /translation="TYSFYQLGAQASWELDVFGGLRRRREAAGADYAGVQDEADAVRL
                     SVEAEVADTYLQLRGLQARFAIATRQWDTARQLADLVRLRQSQGLASPRELNRVVGEQ
                     AGREAALPPLRAAIAATLNRIDVLAGRQAGTGAPSLSAAAAIPLAPDPSGSIEPAMLL
                     RRRPDIGAAERRLASAYARIGAALSEYYPQVSLGGLAGVAGVGAGNLWDSSAVQAGAL
                     LGVRWRLFDFGRIDAEVSAARGRQAEALAQYRGTVLRATEEVETAFSRLAEGRKEVAA
                     GEREVAALTVSREQARKAYQGGVIALIDVLDADRALLDASNRLADAQAQAARASVAAI
                     RALGGGWQEDRS"
BASE COUNT          833 a         1943 c         1690 g          788 t
ORIGIN      
        1 gctgcgcctg gccggacagc aagcacaccg ccaccttcga attctgcgag aacggcgtca
       61 aggccgtggc ggccgaggcg accggcaagc gggccacgcc gacgctgttc gccgccaaca
      121 ccgtcgcggc cctgatgcgg caaagcgact acgacctcga gcagcacggc cggctgaccg
      181 acccgctggc ctacgatgcg cagaccgaca cgtaccggcc gattgcctgg gacgacgcgt
      241 tcaagctgat cgcccggcac ctgaacggcc tggcctcgcc cgacgatgcc gccttctata
      301 cctcgggccg ggccagcaac gaagcggctt ttctgtacca gctgttcgtg cggatgttcg
      361 gcaccaacaa cttccccgac tgctcgaaca tgtgccacga ggccaccagc cgcggcctgc
      421 ccgccacggt cggcatcggc aagggcacgg tcacgctgga cgatttcgag ctggccgaca
      481 ccgtcctgat cttcggccag aaccccgcca ccaaccatcc gcgcatgctg ggcgaattgc
      541 gcgcgtgcgc ccggcgcggc gcgacgatcg tctcgatcaa cccgctgcgc gagcgcggcc
      601 tggaacgctt cgccagcccg cagagcccgg tcgagatgct cacgctgtcg agcaccaaga
      661 tcgcctcgac cttcatccag cccaaggtag gcggcgattt cgcgctcatc aagggcgtcg
      721 ccaagcacgt gctggaactc gacgacgagg ccgtcgcgca aggcggcgag cgcgtgctcg
      781 acctcgactt catcgcccgg cacacgcaag gcttcgaggc cttcgccggc gacttgcgcg
      841 ccgaaagctg gcaagtgctg gaatcggagt ccggcgtcgc ccgccgcgac atcgagggcc
      901 tggcgcggat ctacgccgcg ggcaagcgcg tcatcgcgac ctggggcatg ggcatcacgc
      961 agcacaagaa ttccgtggcc accatacgga tgctctccaa cctgatgatg atgcgcggca
     1021 atatcggccg cgaaggcgcg gggctgtgcc cggtgcgcgg gcattccaat gtgcagggca
     1081 accgcacggt gggcatcgag gaaaagccca ccgcggcctt cctcgacagg ctgggcaagg
     1141 ccttcggttt cgagccgccc cgcaggcacg gctacgacgt ggtcgagacg atcgaggcca
     1201 tgctcgaggg ccgcatcaag gttttcatca gcctgggcgg gaatttcgcc atggccacgc
     1261 ccgacacgcc gcgcaccttc gacgccctgc gccgctgcga cctgacggtg cagatcgcca
     1321 ccaagctcaa ccgcagccac ctggtgcacg gcaaggacgc gctgatcctg ccgaccctgg
     1381 gccgcaccga gatcgacctg caggacggcg tgccgcaagg catcaccgtc gaggattcga
     1441 tgagcatggt gcatgtgtcc tacggcatga acccgcccgc ctccggccag ctgaaatcgg
     1501 agaccgccat cgtcgccggc atggccgatg ccacgctcgg cggcggccgg gccgactggc
     1561 tgggcatggc gtccgactat gcgcgcatcc gcgacgcgat cgaggcgtcc atcgccggct
     1621 tcgaccgcta caacgagcgc atcgcccagc ccggcggctt tcacctgcgg gtcgcgtccc
     1681 gggaaaggga ctggaagacc gatacgggca aggccaattt catggtgctg cccgtcgagg
     1741 aagacacgcc catccggcgc gcccgcgcgc tgcacggcga gcgcctgatg gtgctcatga
     1801 ccacgcgctc gcacgaccag tacaacacca ccatctacgc catggacgac cgctaccgcg
     1861 gggtcttcgg ccagcgccgc gtggtgttca tcaaccccgc cgacctggag atgctgggtt
     1921 tcaaggccgg cgaatgggtc gacatgagca cggtctggga cgacggcatc gaacggcggg
     1981 ccgactcgtt cctgctggtc gaatacgaca tcccgcgcgg ctgcatcggc gcctattacc
     2041 ccgagaccaa ccccctggtg ccgctggcca gcgtggccga cgtctgcaat acgccgacct
     2101 cgaaatccat ccccgtgctg ctgcgccggg gcatgcccct cgccgcctga gctccaccgc
     2161 catgatcatc agacccaccc agcactggct gcggctgctc ttcgtctggc acggctcggt
     2221 cctgcaatcc atcctgccgc agctgctgct gatgaccggc atcggcgtcg ccgcggtgct
     2281 gacgcacggc caggtcttcg gggaaaaagt gccgctgaac gcgacgccgt tcacgctgtg
     2341 cggcgtggcc ctgacgatct tcatggcctt tcgcaacaac gcgagctatg cccgctatgt
     2401 cgaagcccgg cacctgtggg gcaacttgct gatcgcgacg cgcacgctga cttcccaggc
     2461 gctggcctac atcccgcccg gcgcggggtt cgcccccagg ccgttcacgc acgagctgat
     2521 cggcttcgtc cacgcgctca agcaccagtt gcgcaagacc gacccggccc aggcgctcgc
     2581 cgcgcacctg gaaccggcgc tcgcgcagca gctggcgcgc cagcattacc gccccatcgc
     2641 catcctgcgc gacgtccgcc agcgcctgtc cggcctgcag cgccgggccg tcatcaccga
     2701 caaccagctc tggatgctcg acacgcaact ggtggagatg gaaaaaaccg tgggcggctg
     2761 cgaacgcctc gcctccacgc ccatcccctt cgcctacggg gtgctgctgc accgcaccgt
     2821 ctacgcctat tgcgcgctgc tgcccttcgg gctggtcgat tcgacgggcg tcttcacgcc
     2881 cttcgtctcg gtcttcgtcg cctacacctt cctcgccctg gaagccatcg ccagcgaaat
     2941 caccgacccg ttcggcaccg cgcccaacca cctcgcgctc aacgccatca cgtgcacgat
     3001 cgagcgttcg ctgctggaac tgagcgggga aaccatgccc gacgaggcaa agccggcgcg
     3061 gcgctaccag ttgacctgac aggcccgcgg gcggcgcggc tcgctagcga tggcgacccg
     3121 tcaggccggc cgcctgaaca ccacgagctt cgcggtcgat tgctggacga tctcgccgcg
     3181 ctggttcagc gtgttgctct ggcacagcac gatggcccgg tccggcttgg acttcgacgg
     3241 cgtgatcgcc agcacggtgg tctccacccg cagcgtgtcg cccggacgca cgggacgggg
     3301 ccagctcaat tcggtgccgg cgccgatgag gccggtggcg atcggcatcc cgccgtcgac
     3361 cagcaggcgc atggtgaccg ccgccgtgtg ccagccgctg gcggccagcc cgccgaacag
     3421 ggtctcccgc gcccgggcgt cgtcgaggtg gaacacctgc gggtcgtatt gccgggcgaa
     3481 ctgcgtgatc tccgctacgc tcatcgtgtg cgtgccgctg cgaaacaccg cgccgacggc
     3541 caggtcttcc aggtagatgc ctggagcggg ggtttgcgtc atgatgtggg actcctcgaa
     3601 tcggtagtgg atgccgtagc gtttgctagg ccaggcccag ccgctctttc aggcgttcgc
     3661 gcgatgcatg caggatatcg cggccttcct tttcttccgg cgtggcccgg gccagggaca
     3721 tggcgcctat catttccgca agcacggacg aggccagcga ttgcgcatcg tccctgccca
     3781 tgtccgtcaa cgccgccgta atcgcttgcc tgaaatggcg cacgccgcga tcaaagcgcg
     3841 cccgcgcggc cgccggcatg cggctggcct cgcccgtcag cccgggcaag gggcagccgc
     3901 ggtcgaccga cttgcgcaag cggtcggaaa gatagaaatc gatcagggcc gccagccccg
     3961 aggcgccgtc cttgccgtgc aggcaccgct ccagcatggc cccggtgtcc ttgaacatgc
     4021 agtcgactgc ttcctcgacc agttcgtcgc gggacgcgaa atgcgcgtag aagccgccgt
     4081 gggtcaggcc cgcgcgcttc atcagcgcgg cgacgccgat gccgtcccag ccatgctcgc
     4141 gcatggcgct ggccgccgct tcgacgatgc gcgcatgggt gcgctcttta tgcgaaagtt
     4201 tttccggcat ggttcaactc ctgtcctcct gccatccgcc gcccagggcg cggatcgccg
     4261 ctacggaagc ccgcgcggcc tgcgcctgcg catccgcgag acggttggac gcatcgagca
     4321 gcgcccggtc ggcatccagc acgtcgatca gggcgatcac gccgccctgg taggccttgc
     4381 gcgcctgctc ccgggacacg gtcaacgccg cgacttcccg ttcgccggct gccacttctt
     4441 tcctgccctc ggccaggcgg gagaaagccg tttcgacttc ctcggtggcg cgcagcaccg
     4501 tgccccggta ttgggccagc gcctcggcct gccggccgcg cgccgccgac acctcggcat
     4561 cgatgcggcc gaaatcgaac agccgccacc ggacacccag caaggcgccc gcctgcaccg
     4621 cgctggaatc ccacaggttt ccagcgccga cgccggccac gcccgccagg ccgcctagcg
     4681 agacttgcgg ataatactcg gacagggccg cgccgatgcg ggcataggcc gacgccagcc
     4741 ggcgttcggc cgcgccgatg tcgggccggc ggcgcagcag catagccggc tcgatgctgc
     4801 cgctcgggtc cggcgcaagc ggaatggccg ccgcggccga cagcgacggc gcgcccgttc
     4861 ccgcctgccg gccggcgagc acgtcgatcc ggttcagcgt cgcggcgatc gcggcgcgca
     4921 agggcggcag cgcggcttcg cggcccgcct gttcgccgac gacccggttc agctcccgcg
     4981 gagaggccaa cccctgggac tgccgcagcc ggaccaggtc cgccagctgg cgcgccgtgt
     5041 cccattgccg ggtggcgatc gcgaaccgcg cctgcagccc gcgcagttgc agataggtat
     5101 cggccacttc cgcctcgacg ctcaggcgga ccgcatcggc ctcatcctgg acgcccgcgt
     5161 agtcggcgcc cgccgcctcg cgccggcggc gcagcccgcc gaacacgtcc agctcccagg
     5221 acgcctgggc cccgagctgg tagaacgaat aagt
//
LOCUS       DASWPO010000239         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1665800, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000239 DASWPO010000000
VERSION     DASWPO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665800"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VGC69_12560"
     CDS             <1..694
                     /locus_tag="VGC69_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0296156.1"
                     /translation="IHLAFNHDFSTFAANCEDDRRVIEALGSALAGSDRPLVVTSGTG
                     MANAVPGQPAREDDATISSDVIPRAASEEAAAAAAAQGVNVSVVRLPQVHDTARQGLV
                     TPAIGIYREKGVCAYVGDGLNRWPAAHVLDVARLYRLAIEKAEPNARYNAVAEEGVPV
                     RDIAATLGRRLHLPVESVTAEEAGAFFGWLAMFAGLDLPASSEQTRKKLGWQPTGPGL
                     IADLERLQVADA"
     gene            complement(707..1189)
                     /locus_tag="VGC69_12565"
     CDS             complement(707..1189)
                     /locus_tag="VGC69_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY0296157.1"
                     /translation="MPSFDVVSEVDKHELTNAVDQASRELTTRFDFKGIDASFELEGY
                     VVSLAAASAFQIKQMLDILRGRLSGRGIDVRCLEPGEMVENLGGARQKVTIKQGIEQA
                     VAKKLVAAIKGSKIKVDSQINGDKLRITGKKRDDLQAVIALLRKTDVELPLQFQNFRD
                     "
     gene            complement(1248..1976)
                     /locus_tag="VGC69_12570"
     CDS             complement(1248..1976)
                     /locus_tag="VGC69_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0296158.1"
                     /translation="MPGYWASWCRRAACAALLAALAACSSTPSSSGKSPAYYRVKEGD
                     TLSQIAQKHDQSTGDLLRWNKLTDANQITPGMLLRVRPPFHDASSKGDGAPPPRASGK
                     GDKPAPRTGAAAPASAPVHGITLVWPAQGKLLAGYNGSSSKGLTITGSTGAPVVAAAP
                     GTVAYANSGLRGYGNLVIVRHENNFLTIYAHNSRLLVKQGDRVKRGQRIAEMGSSDAR
                     RVELYFELRQGGQPVNPMGVLPKQ"
BASE COUNT          345 a          718 c          702 g          358 t
ORIGIN      
        1 catccacctg gccttcaacc acgatttctc gacattcgcg gcgaactgcg aggatgaccg
       61 gcgtgtcatc gaggcgctcg gctcggcgct ggccggctcc gaccggccgc tggtcgtcac
      121 ctccggcacc gggatggcga atgccgtccc cggccagccg gccagggaag acgatgcgac
      181 gatcagctcg gacgtgattc ctcgcgccgc ctcggaagaa gccgccgccg ctgccgccgc
      241 gcaaggggtc aatgtgtcgg tggtgcggct cccgcaagtc cacgacacgg ccaggcaggg
      301 cctcgtcacc ccggcgatcg ggatatatcg cgaaaaaggc gtgtgcgctt atgtcgggga
      361 cggactcaat cgctggccgg cggcgcacgt cctcgacgtg gcccgcctct acaggctggc
      421 gatcgaaaag gccgagccga acgcaagata caacgcggtg gcggaagaag gcgtgccggt
      481 gcgcgatatt gccgcaaccc tgggccggcg cctgcacctg ccggtcgaat ccgtcaccgc
      541 cgaagaggcc ggcgcctttt tcggctggct cgcgatgttt gccgggctcg acctgcccgc
      601 ctccagcgag caaacgcgga aaaaactcgg atggcagccg accgggcccg gcttgattgc
      661 cgatctcgaa cggctgcagg tcgccgacgc ctgaggcagc gcctcgtcag tcccggaaat
      721 tctggaactg cagcgggagt tcgacgtcgg tcttgcgcag cagggcgatg accgcctgca
      781 ggtcgtcgcg cttcttgccg gtgatccgca gcttgtcgcc gttgatctgg ctgtccacct
      841 tgatcttgct gcccttgatc gcggccacca gcttcttggc cacggcttgc tcgatcccct
      901 gcttgatggt gaccttctgg cgcgcgccgc ccaggttctc gaccatctcg cccggctcca
      961 ggcagcgcac atcgatgccg cggcccgaca ggcgcccgcg caggatgtcg agcatctgct
     1021 tgatctggaa agccgaggcg gccgcgaggc tcaccacata gccctccagc tcgaaactgg
     1081 catcgattcc cttgaaatcg aaccgcgtgg tcagctcgcg gctggcttgg tcgaccgcat
     1141 tggtcagttc gtgcttgtcg acctcggaga cgacgtcgaa actcggcatg gcgcaaatcc
     1201 tgtcatcaaa aaggcatgcc tgccagttta accgcccggg aggctgctta ttgcttgggc
     1261 agcaccccca tcggattgac cggctgcccg ccctggcgca gctcgaaata cagctcgacc
     1321 cggcgcgcat cgctgctgcc catttcggcg atgcgctggc cccgcttgac gcggtcgccc
     1381 tgcttgacca gcagcctgct attgtgcgcg tagatcgtca ggaagttgtt ttcgtgccgc
     1441 acgatcacca ggttgccgta gccgcgcagc ccgctgttgg cataggccac cgtgcctggc
     1501 gccgcggcca ccaccggcgc gccggtggaa ccggtgatgg tcagcccctt ggagctcgaa
     1561 ccgttgtagc cggccagtag cttgccctgg gccggccaga cgagcgtgat gccatgcacc
     1621 ggcgccgacg cgggcgccgc cgcccccgtg cgcggcgcgg gcttgtcgcc cttgcccgag
     1681 gcgcgcggcg gcggcgcgcc gtcgcccttg ctgctggcat cgtgaaacgg cggccgcacc
     1741 cgcagcagca tgcccggcgt gatctggttg gcgtcggtga gcttgttcca gcgcagcagg
     1801 tcgcccgtgc tctggtcgtg cttctgggcg atttgcgaca gcgtgtcgcc ctctttgacg
     1861 cggtaatagg cgggagactt gcccgacgac gagggggtgc tgctgcaggc ggccaatgcg
     1921 gccagcaggg cggcacaggc ggcccgcctg caccaggagg cccagtaccc cggcatggcc
     1981 gtggaaatca cgcgaaaaca ggacgaaaag aaagcgggag aagcggacac ggaatcgaac
     2041 gcagtggcag accggcctaa gagtatcagg aagtcggcgc ccgccataag tcttgacgat
     2101 tgataacgcc tgcccatccg gga
//
LOCUS       DASWPO010000240         7490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_324649, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000240 DASWPO010000000
VERSION     DASWPO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324649"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VGC69_12575"
     CDS             complement(<1..694)
                     /locus_tag="VGC69_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0296159.1"
                     /translation="MSISTASCGRLDIPSVRDQVSAEEWEARVDLAACYRLMHHFGMT
                     EMIANHISVRVPGTEDRFLINPYGMFYDEITASSLIEIDLEGNELRNTTPYHANKVGF
                     IIHSAIHRNARHLHCVIHTHTIAGMAVAALEEGLIPMSQTAMRFGRIGYHKYEGVVLN
                     MEEQARLVADLGDNEAMILRNHGLLTAGETIQEAFNTMFRLERACQLQVAALSCGRKL
                     SLPSEELVQETYR"
     gene            complement(706..1674)
                     /locus_tag="VGC69_12580"
     CDS             complement(706..1674)
                     /locus_tag="VGC69_12580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296160.1"
                     /translation="MIHRTLLFLASVLLMVPATASLAAYPEKPIRIIIPFDAGGPTDV
                     LIRSIAPILTQDLGQNIVIENKGGAGSVIGVGYVAHAKPDGYTLVVGGSPTPINASFM
                     PNLPYDTEKDLTAIAPLARTPYFLIAGSKFPVNSVKDLIAYAKAHPGQVTYASSGIGN
                     RPHLAGAQFGILTGTQLLHVPFKGTAPATTALLSGEVNIMFAGLATVLPNLQAGQLKV
                     LAVATPKRSSFLPNAPTLGEEGIPNFYPDVWYGLLAPGGTPQAIKDRLAQAVNKALKN
                     PDTVKLYASLGAVPFISTPAQFQTFFDGEVNEWRDFFKTNPSLLKE"
     gene            complement(1861..2781)
                     /locus_tag="VGC69_12585"
     CDS             complement(1861..2781)
                     /locus_tag="VGC69_12585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296161.1"
                     /translation="MRTRLTVQELEKFIDVAETLSFRASAARCSVSQPALSRTIASAE
                     AKLGTRLFDRNTHEVTLTQSGRQLLPIARRIVYELHDSLSDLAEFVAGRSGRISIASI
                     PSVAASVLPDSMQRFLQAYPNVSIGLRSVSAEEVLSNVADGSADIGVSALTFDQATLE
                     ASRFAFTPFRKDDLLLICAEDDPLASRKSVTWEVFTQRPYIANGATSSIRLLAERVFA
                     NTPGQLQPRYESFNISVTARMVAAGLGIAVMPSLAKDIVNTQGLAFLKLTDPVVSRNI
                     GIFTLKNRSLAVPTQNFLRYLHGDASALSQ"
     gene            complement(2838..3731)
                     /locus_tag="VGC69_12590"
     CDS             complement(2838..3731)
                     /locus_tag="VGC69_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296162.1"
                     /translation="MNTEFIKTLVAVARHGSMAEAARRLGLTHGAVAQQIRVLEKDLG
                     ADLVARAGRTVHLTEKGAELLGHFDQMLEQVEKIRYMAKSNELMGELRLGAGMTALTT
                     VVPRILERLVKQDLLINVDIQPGHSVSFYPEIEAGRLDIAIALEAPYELPKSQSWHLL
                     REEPYVLVAAARHAGKDPHRLLATQPLIRYSLSDWGGRHADEYLHRANIQPNVRFELN
                     AIESIAVMISRDLGVAILPVSTSGAIRRLKLATIPLPRPCEPRRVGLIWQRASPRASL
                     IKVFLDAAVVEYKRYAIQMSS"
     gene            3819..4673
                     /locus_tag="VGC69_12595"
     CDS             3819..4673
                     /locus_tag="VGC69_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY0296163.1"
                     /translation="MRSKLFVPASRPELFEKAMKSMADSISFDLEDAVVPEAKESARH
                     TLAAYLRGADFQKKEKKIIVRINAADTPYFQDDLRVLAGAPVDILNVPKLECADDVRH
                     AVTAVEAAGFADTDVALLANIETPRALRLAAEIAAAHPRVCGLQLGLADLFEPYGIAR
                     YEAGTLRQVMLALRLAAGECGKYALDGAYARAADTQGFRDEALLARSLGLMGKSCIHP
                     NQIAIANEVFGPSAQDIEHAARVMQASRRNAGAGAFLLDGQMIDAPFVKRARETLRLA
                     GLDPDVDQ"
     gene            4690..5883
                     /locus_tag="VGC69_12600"
     CDS             4690..5883
                     /locus_tag="VGC69_12600"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0296164.1"
                     /translation="MTTDILPLSGVKVLDLSAYIAGPYGCTLLGDLGADVIKVEPPEG
                     DNLRKYPSTIEAESRAFLGLNRNKRGLCLDLKNPDGHAVLIRLVRQADVLVHNFRPGV
                     PERLRIDFETLRAANPRLVYCAMTGYGDRGPLARNAGYDQVMQSMTGMCASQGKAGGP
                     PEILYGSIVDYYGASMIAGTVASALYQRERTGAGLAVQVSLLGSALCMQSARLVWADG
                     EPRDIERDMRSGGITGIHPTKDGYIYLSANTPHFWHSLCEHLGLAELADDERYDSVKK
                     RAVHAGVLVSRVRAALAGATAHEWAAKFGQSVPCSVVNRVENMFDDPQVQAMDFMGTY
                     ANERVGGYRGLARWASFDGDAQPAAGRHEGGAPGLGEHSRQVLRDLGYDETAIDRLFA
                     STAVR"
     gene            complement(5939..6937)
                     /gene="miaA"
                     /locus_tag="VGC69_12605"
     CDS             complement(5939..6937)
                     /gene="miaA"
                     /locus_tag="VGC69_12605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY0296165.1"
                     /translation="MPDSASTLPVICLAGPTAAGKSAACLALAQRWPLEIINVDSATL
                     YRGMDIGTAKPDAAERALAPQHLLDIRDPAESYSAAQFREDALRLIAEIRARGRLPLL
                     AGGTMLYYKALRDGLDDLPQADPALRAELEAEAARHGWPHLHARLAALDPATAARLSP
                     NDSQRIQRALEICLLSGKPMSELLSGTTPSAAASAPGLQRKAGAADTPHYLTISLEPA
                     DRLALHARIARRFDAMLAAGFLDEVRALRARGDLHPGLPAIRCVGYRQLWAHLDGETT
                     LEAAREQGIAATRQLAKRQITWLRAQPDRIRIDCMAADAVAQAVDAAATSLSRRAA"
     gene            complement(6991..>7490)
                     /gene="mutL"
                     /locus_tag="VGC69_12610"
     CDS             complement(6991..>7490)
                     /gene="mutL"
                     /locus_tag="VGC69_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929705.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEY0296166.1"
                     /translation="HAAHERVVYEQLKRELDTRGLPRQDLLVPVVFHAAEKDVALVEE
                     HAEQLAELGFEMRPAGPASIAVRCVPAMLARGDIETLARAVLRDLAAVGVSQLLTEQR
                     NELLSTMACHGSVRANRKLTLDEMNALLRQMEATERADQCNHGRPTWVQWSVNELDKL
                     FMRGQ"
BASE COUNT         1212 a         2264 c         2688 g         1326 t
ORIGIN      
        1 tccggtaggt ctcctgcacc agttcctcgg agggcaggct gagcttcctg ccgcacgaca
       61 aggccgccac ctggagctgg caggcgcgct ccagccggaa catggtgttg aaggcttcct
      121 ggatcgtctc gcccgccgtc agcaagccgt gattgcgcag gatcatggcc tcgttgtcgc
      181 cgaggtcggc caccaggcgg gcctgttctt ccatgttgag gacgacgcct tcgtacttgt
      241 ggtagccgat ccggccaaag cgcatggcgg tctgggacat ggggatcagg ccttcctcca
      301 gcgccgcgac ggccatcccg gcaatggtgt gcgtgtggat gacgcagtgc aaatgcctgg
      361 cgttgcggtg gatggcgctg tggatgatga agccgacctt gttggcatgg tagggcgtcg
      421 tgttgcgcag ttcgttgccc tccagatcga tctcgatcag gctggaggcc gtgatctcgt
      481 cgtagaacat gccgtaggga ttgatcagga atctgtcttc ggtccccggc acgcgcaccg
      541 agatatggtt ggcgatcatc tccgtcatgc cgaagtggtg catgagccga tagcaggcgg
      601 ctaggtcgac gcgggcctcc cactcttcgg cgctcacttg atcacgcacg ctggggatgt
      661 ccaggcgacc gcaagaggct gtagagatgg acatgatgga ttcctttact ctttcaacaa
      721 tgagggattg gtcttgaaaa agtcccgcca ttcgttgacc tcgccgtcaa agaatgtctg
      781 gaactgagcc ggtgtggaaa tgaacggcac ggcgcccagg ctcgcgtaga gcttgaccgt
      841 gtcggggttc ttcaaggctt tgttcaccgc ctgggcgagc ctgtccttga tggcctgggg
      901 cgtgccgccg ggagcgagca gcccatacca gacgtccggg tagaaattgg gaatgccttc
      961 ctcgcccagc gtgggggcgt tcggcaggaa ggaggagcgc ttgggcgtgg cgaccgccag
     1021 caccttgagt tggccggcct gcagattggg cagcacggtg gccaggccgg cgaacatgat
     1081 gttgacctcg ccggagagca gcgccgtggt ggcgggcgcg gttcccttga acggcacatg
     1141 cagcagttgc gtgcccgtca ggatgccgaa ctgggcgccc gccaggtgcg gccggttgcc
     1201 gatgccggag gaggcatagg tgacctggcc gggatgcgct ttggcgtagg cgatcaaatc
     1261 cttgaccgag ttgaccggga acttgctgcc ggcaatcagg aagtagggcg tgcgggccag
     1321 cggggcgatc gccgtcaggt ccttctccgt gtcatacggc aggttgggca tgaagctggc
     1381 gttgatgggc gtgggcgatc cgcctaccac cagggtgtag ccgtccggtt tggcatgggc
     1441 cacgtagccg acgccgatga cgctgcccgc tccgcctttg ttttcgatca cgatgttctg
     1501 gccgaggtcc tgcgtcagaa tcggggcaat cgaccggatc agcacgtcgg tcgggccgcc
     1561 ggcgtcgaag ggaatgatga tgcgtatggg tttttccgga taagcggcca ggctggcggt
     1621 ggcgggcacc atcagcagga cggacgccag gaaaagcaag gtgcggtgga tcatgatcgt
     1681 tcttcttgtg cggatgcgtt tttgggaatc gcgcgctgcg ggcattcccc ggcggcaggc
     1741 agaagcctcg tcacgcatgc ggtgacgagg ctcccgacgc gcactgcacc ggtgtctcgt
     1801 ggaaagccgt ggggagatta tctcggcgaa ggccgagata agggaattgc tatcttctgt
     1861 tcactgcgat aacgcagacg catcaccgtg caggtatcgc aggaagttct gcgtcggcac
     1921 ggcgagcgac cggttcttga gcgtgaagat gccgatgttg cgggacacga ccggatcggt
     1981 cagcttcagg aaggccaggc cctgggtgtt gacgatgtcc ttggcgagcg acggcatgac
     2041 cgcgatgccc aggccggcgg cgaccatgcg cgccgtgacc gagatattga atgattcgta
     2101 gcggggctgg agctggccgg gcgtgttcgc gaagacccgc tcggccagca gcctgatgct
     2161 gctggtggcg ccgttggcaa tgtagggccg ctgcgtgaac acttcccagg tcacggactt
     2221 ccggctcgcc aggggatcgt cctcggcgca gatgagcagc aggtcatcct tgcggaacgg
     2281 cgtgaaggcg aatcggctgg cttccagggt ggcctggtcg aaggtcagcg cggatacgcc
     2341 gatgtcggcc gatccatcgg cgacattgct caggacttct tcggccgata ccgagcgcaa
     2401 gccgatcgag acgttcggat acgcctgcag gaagcgctgc atcgagtccg gcagcaccga
     2461 ggcggcgacg gaggggatgc tggcgatcga tatccgcccg cttcggccgg cgacgaactc
     2521 cgccaggtcg ctgagcgaat cgtgcagttc atagacgatg cggcgcgcga tgggcagcag
     2581 ctgccggccc gactgggtca gcgtgacctc gtgcgtgttg cggtcgaaca ggcgtgttcc
     2641 cagcttggcc tcggcggagg cgatggtgcg gctgagcgcc ggctgcgaaa cgctgcatct
     2701 cgccgccgat gcccggaaac tgagggtctc ggcgacgtcg atgaattttt ccagttcctg
     2761 gacggtcaag cgggtgcgca tggtcttgct gtttttctcg cggcggggga aatgcctggc
     2821 gccccaggag cgccgtgcta cgacgacatc tggatggcgt accgtttgta ctctactacg
     2881 gccgcgtcca ggaacacctt gatcaggctg gcgcggggcg aggcgcgctg ccagatcaag
     2941 ccgacgcggc gcggctcgca aggccgcggc agggggatgg tggccaattt gagccgcctg
     3001 atggcgccgc tggtggagac gggcaggatg gccacgccga ggtcccggct gatcatgacg
     3061 gcgatcgatt cgatcgcatt gagttcgaag cgcacattgg gctggatatt ggcccgatgc
     3121 agatactcgt cggcatggcg cccgccccaa tcgctcaggc tataccggat cagcggctgc
     3181 gtggccagca gccggtgggg gtccttgccg gcatggcgcg ccgccgccac caggacatag
     3241 ggttcttcgc gcagcaagtg ccagctctgg ctcttgggca attcgtacgg cgcctccagc
     3301 gcgatggcga tatcgagtct gcccgcctcg atctccggat agaagctgac cgagtgcccc
     3361 ggctggatat ccacgttgat gagcaggtcc tgcttgacga ggcgttcgag tatgcggggg
     3421 accacggtgg tcaatgcggt catccccgcg cccaggcgca gttcgcccat gagctcgttg
     3481 gatttggcca tatagcggat cttttccacc tgttcgagca tctggtcgaa atgcccgagc
     3541 agttcggcgc ctttttccgt caggtgcacg gtgcggccgg cgcgcgcgac caggtcggcc
     3601 cccaggtcct tttccaggac gcggatctgc tgcgccaccg cgccatgcgt caggcccagg
     3661 cgccgggctg cctcggccat ggagccatgg cgggccacgg cgaccagggt tttgatgaac
     3721 tcggtattca tgacgatagg aaagttatct tcttatcgaa ccaatcatac acatatcgaa
     3781 ggcccgccct ttgccaaaat aggaaaaagg agacaatcat gagaagcaag ctgttcgttc
     3841 cggcgtcccg tccagaactg ttcgagaagg ccatgaaaag catggcggat tcgatctcgt
     3901 tcgatctgga ggacgccgtg gtgccagagg ccaaggagtc cgcccgccat acgctggcgg
     3961 cttatctgcg cggcgccgat ttccagaaga aagaaaaaaa aattatcgtt cggatcaatg
     4021 cggccgatac gccctacttc caggacgatt tgcgggtcct ggccggcgcg ccggtcgaca
     4081 tcctgaacgt gccgaagctg gaatgcgccg atgacgtccg ccatgcggtg acggccgtcg
     4141 aggcggccgg gttcgcggac acggacgtgg cgctgctggc caatatcgaa accccgcgcg
     4201 ccttgcgcct ggccgccgaa atcgccgccg cgcatccccg cgtgtgcggc ctgcagctgg
     4261 gcctggccga tttgttcgag ccttacggca tcgcgcgata cgaagccggc accttgcggc
     4321 aggtcatgct ggccttgcgg ctggccgccg gggagtgcgg aaaatacgcg ctggacggcg
     4381 cttacgccag ggcggccgac acgcagggct tccgggacga agccttgctg gcccgctccc
     4441 tgggcctgat gggcaagtcc tgcatacacc ccaatcagat tgcgattgcc aacgaggtgt
     4501 tcggcccttc ggcccaggac atcgagcacg ccgcgcgcgt catgcaggca agccgccgga
     4561 acgccggcgc cggcgcgttt ttgctcgacg ggcaaatgat agacgcgcct ttcgtcaagc
     4621 gtgcccgcga aaccctgcgc ctggcaggac tcgatcccga tgtggatcaa tgaattcgga
     4681 ccgatgacca tgacaaccga tatccttccc ttgtccggcg tgaaggtgct cgatttgagt
     4741 gcctatatcg ccggtccgta tggctgcacg ctgctcggcg atctgggcgc ggacgtgatc
     4801 aaggtggagc cgccggaagg cgacaacctg cgcaaatatc cctccaccat cgaagccgag
     4861 agccgggctt tcctgggcct gaaccgcaac aagcgcggcc tgtgcctgga cctgaagaat
     4921 cctgacggcc atgccgtgct gatccggctg gtgcgccagg ccgacgtgct ggtgcacaat
     4981 ttccgtcccg gcgtgccgga acggctgcgc atcgatttcg aaaccctgcg ggcggcaaat
     5041 ccgcgcctcg tctattgcgc catgacggga tacggggatc gcggccccct ggcgagaaac
     5101 gcgggctacg accaggtaat gcagtccatg accggcatgt gcgcctcgca gggcaaggcc
     5161 ggcgggccgc ccgagattct gtacggctcc atcgtcgact attacggcgc atcgatgata
     5221 gccggcacgg tcgccagcgc gctgtatcag cgcgaacgga ccggcgcggg cctggccgtg
     5281 caggtatcgc tgctgggcag cgccttgtgc atgcagtcgg cgcggctggt atgggccgat
     5341 ggagagccgc gcgacatcga gcgcgatatg cgttccggcg gcatcaccgg gatacatccg
     5401 accaaggacg gctatatcta cctgtcggcc aacacgccgc atttctggca ttccttgtgc
     5461 gagcatctgg gcctggccga gctggccgac gacgaacggt acgacagcgt caagaagcgc
     5521 gccgtccatg ccggcgtcct ggtgtcccgg gtgcgcgccg ccctggccgg cgcgacggcg
     5581 catgaatggg cggccaaatt cgggcagtcc gtgccgtgtt ccgtggtcaa ccgcgtggag
     5641 aatatgttcg acgatcccca ggttcaggcc atggatttca tggggaccta tgccaacgag
     5701 cgcgtgggcg gctatcgcgg cctggcccgc tgggccagct tcgacggcga tgcccagccg
     5761 gctgccgggc gccatgaagg cggcgcgccc ggtctgggcg agcacagccg ccaggtattg
     5821 cgcgacctgg gctatgacga gaccgccatc gaccggttgt ttgcatccac cgccgtgcgg
     5881 tgacgggtcg gcgtcgagct tgccaggggt cctgaaggcc gggtctggcg ccggggattc
     5941 aggcagcccg gcgtgagagg ctcgtggccg cggcatccac cgcttgagcg acggcgtcgg
     6001 cggccatgca atcgatccgg attctatccg gctgcgcgcg cagccaggtg atctgccgct
     6061 tggcgagttg ccgcgtcgcg gcgatgcctt gttcccgcgc tgcttcgagc gtggtctcgc
     6121 cgtccaggtg ggcccagagc tggcgatagc ccacgcagcg gatggcgggc aggccggggt
     6181 gcaggtcgcc gcgcgcgcgc agggcgcgga cttcgtcgag gaagccggcc gcgagcatcg
     6241 cgtcgaagcg ccgggcgatg cgggcgtgca gggccaggcg gtcggcgggt tcgagactga
     6301 tggtgaggta gtgcggcgtg tccgcggcgc cggccttgcg ttgcaggccg ggcgccgagg
     6361 cggcggcgct gggcgtggtg ccgctcagca gctcggacat gggcttgccg ctcagcaggc
     6421 agatttccag cgcgcgctgg atgcgctggc tgtcgttggg cgagagacgg gccgcggtgg
     6481 ccggatccag cgcggcaagc cgcgcgtgca aatgcggcca gccatgccgg gccgcctcgg
     6541 cttcgagttc ggcgcgcagc gcggggtcgg cctggggcag gtcgtcgagg ccgtcgcgca
     6601 gggccttgta gtagagcatg gtgccgccgg ccagcagcgg caggcggccg cgggcgcgga
     6661 tctcggcgat gaggcgcagg gcgtcttcgc ggaactgcgc ggcggaatag gattcggcgg
     6721 ggtcgcggat gtcgagcagg tgctggggcg ccagggcgcg ctcggccgcg tcgggcttgg
     6781 cggtgccgat gtccatgccg cggtagaggg tggccgagtc gacgttgatg atctccagcg
     6841 gccagcgctg cgccagggcg aggcaggccg cgctcttgcc ggcggcggtg gggccggcga
     6901 ggcagatgac gggaagcgtc gaggcggaat cgggcacgga cggggcgcgg cgttcagggc
     6961 gtagggaatg cggaggctgg gcgcgcgcgc ctactgcccg cgcatgaaaa gcttgtccag
     7021 ctcgttcacg ctccactgca cccaggtggg ccggccgtgg ttgcactggt cggcgcgctc
     7081 ggtggcttcc atctggcgca gcagggcgtt catctcgtcg agcgtgagct tgcggttggc
     7141 acgcaccgaa ccgtggcagg ccatggtgga gagcagttcg ttgcgctgct cggtgagcag
     7201 ctgcgagacg ccgacggcgg ccaggtcgcg cagcacggcg cgcgccaggg tctcgatgtc
     7261 gccgcgcgcc agcatggccg gcacgcagcg cacggcgatc gacgcggggc cggcggggcg
     7321 catttcgaag ccgagctcgg ccagttgttc cgcgtgctct tcgaccaggg ccacgtcttt
     7381 ctcggcagcg tggaacacga cgggcacgag caggtcctgg cggggcaggc cgcgggtgtc
     7441 gagctcgcgc ttgagctgtt cgtagaccac gcgttcatgg gcggcatgca
//
LOCUS       DASWPO010000241         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_415094, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000241 DASWPO010000000
VERSION     DASWPO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415094"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1092)
                     /locus_tag="VGC69_12615"
     CDS             complement(115..1092)
                     /locus_tag="VGC69_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296167.1"
                     /translation="MNRQLLAYALAAIVVAALPYLGVYPIFVMEVLCYALFACAFNLL
                     LGYTGLLSFGHAAFLGFAGYAAGQALKVWGFPTELGILFGTAAAALLGLVMGLLAIRR
                     SGIYFAMITLAMAQMVFFFFLQAPFTGGENGLQQIPRGKALGFIDLADDLHLYYLVLI
                     VFAIGYFIIWRAVHSPFGQVLKALRENEPRAISLGYDVDRFKLLAFVLSAALAGLAGA
                     TKALVFVSATLSDATWQMSGLVILMTLIGGMGTLVGPILGAFIVVLLENKVGDFGTFL
                     AKLTHVDWFNQLGESVTIVIGLIFVVCVLAFRRGIVGELLAWNQRRRTV"
     gene            complement(1089..1976)
                     /locus_tag="VGC69_12620"
     CDS             complement(1089..1976)
                     /locus_tag="VGC69_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296168.1"
                     /translation="MISLFGIPIQALLGQLLLGLVNGSFYAILSLGLAVIFGLLNVIN
                     FAHGALYMTGAFIAWMGMSYLGLNYWIMLILAPIVVGLFGIVIERLLLKPLARLDNLY
                     GLLLTFGLALLIEGLFRSVYGVSGQPFPPPAALRGATNLGFMVLPNYRAWVVVASVVV
                     CLATWFVIERTRLGSLLRAATENPKLVEAFGVNVPRMITLTYGFGVALAGFAGVLAAP
                     VLQVAPLMGSNLIIVVFAVVVIGGMGSILGAIVTGLGLGIIEGFTKVFRPEASSTVVF
                     IIMVVVLLLRPTGLFGKEK"
     gene            complement(2050..>2997)
                     /locus_tag="VGC69_12625"
     CDS             complement(2050..>2997)
                     /locus_tag="VGC69_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296169.1"
                     /translation="AREWFDQQKVDMLIGGTNSGTGLSMAVVAAEKHKPFIAIGAGAS
                     ELTTTQCSPYTIHYAYDTVALARGTGSAVVKDGGKSWFFLTADYAFGQALERDTTAVI
                     KATGGTVKGSVRAPLGTTDFSSFMLQAQSSGAQILGLANAGGDTINSVKAANEFGVTA
                     SMKLAGMLVFINDVHALGLDATKGMYLTDGWYWDLNDDTRAWAKKFEAKVGRKPSMLQ
                     AGDYSATMFYLNAVKETGTDDPDTVMKWMKSHKINDFFAKGGYVRADGRMIHDMYLMQ
                     VKTPQESKGPWDLYKVVATLPGDEVYTKPSESPCALLKQ"
BASE COUNT          562 a          920 c         1052 g          463 t
ORIGIN      
        1 gcgccatcag gcgccctcgg cccttcgggc ttccccgccg cagaggcgcc gccccgtgtt
       61 gccccgtttc tccatgctct gttactacta tataacgccc cgggatatac cggcctatac
      121 agttcgccgc cgttggttcc aggccagtag ctcgcccacg atgccgcgcc ggaaggccag
      181 cacgcagacc acgaagatca ggccgatcac gatggtgacg gattcgccga gctggttgaa
      241 ccagtcgaca tgggtgagct tggccaggaa ggtgccgaag tcgcccacct tgttttccag
      301 cagcaccacg atgaaggcgc ccaggatggg gccgacgagc gtgcccatgc cgccgatcag
      361 cgtcatgagg atcaccaggc ccgacatctg ccaggtggcg tcggacaggg tggccgagac
      421 gaacaccagg gccttggtgg cgcccgccag cccggccagc gccgccgaga gcacgaaggc
      481 gagcagcttg aagcggtcga cgtcgtagcc cagcgaaatg gcgcgcggct cgttctcccg
      541 cagggccttg agcacctggc cgaagggcga gtgcaccgcg cgccagatga tgaagtagcc
      601 gatggcgaaa acgatcagca ccaggtagta gaggtgcagg tcgtcggcga ggtcgatgaa
      661 gcccagcgcc ttgccgcgcg ggatctgctg caggccgttc tcgccgccgg tgaagggggc
      721 ctgcaggaag aagaagaaca ccatctgcgc catggccagc gtgatcatgg cgaaatagat
      781 gccgctgcgc cggatggcca gcaggcccat caccaggccg agcagcgcgg ccgcggcggt
      841 gccgaacaga atgcccagtt cggtgggaaa gccccagacc ttgagcgcct ggccggcggc
      901 gtagcccgcg aagcccagga aggcggcgtg gccgaaggac agcaggcccg tatagcccag
      961 cagcaggttg aacgcgcagg cgaacagcgc gtagcagagg acttccatga cgaagatcgg
     1021 atagacgccc aggtagggca gggcggccac cacgatggcc gccagcgcgt aggccaggag
     1081 ttggcggttc atttttcttt tccgaagagc ccggtggggc gcagcagcag cacgacgacc
     1141 atgatgatga aaacgacggt gctcgatgcc tcgggccgga acaccttggt gaagccctcg
     1201 atgatgccca ggcccaggcc ggtgacgatg gcgcccagga tggagcccat gccgccgatc
     1261 accaccacgg cgaacaccac gatgatgagg ttcgaaccca tcagcggggc gacctgcagg
     1321 acgggcgcgg cgagcacgcc ggcaaagccg gccagggcca cgccgaagcc gtaggtgagg
     1381 gtgatcatgc gcggcacgtt cacgccgaag gcctcgacca gcttggggtt ctcggtggcg
     1441 gcgcgcagca gcgaacccag gcgggtgcgc tcgatgacga accaggtggc caggcagacc
     1501 acgacggagg ccaccaccac ccaggcccgg tagttgggca gcaccatgaa gcccaggttg
     1561 gtggcgccgc gcagcgcggc gggcggcgga aagggctggc ccgacacgcc gtagacgctg
     1621 cggaacagcc cttcgatgag cagcgccagg ccgaaggtga gcagcaggcc gtaaagattg
     1681 tcgagcctgg cgagcggctt gagcagcagt ctttcgatga cgatgccgaa caggccgacc
     1741 acgatgggtg cgaggatcag catgatccag tagttcaggc ccagatagct catgcccatc
     1801 caggcgatga aggcgcccgt catgtagagc gcgccgtgcg cgaaattgat gacgttgagc
     1861 aggccgaaga tgaccgccag ccccagggag aggatggcgt agaacgaacc gttcaccagt
     1921 ccgagcagca attgtccgag cagtgcctgg atagggatgc cgaagaggga aatcatcgta
     1981 tgagggtgtt cctacgtggg ttccgcgcgc cgcggcccca ggtaacgggg ccgcggcgga
     2041 tggcatcgct tactgcttga gcagcgcgca gggggattcg gaaggcttgg tgtagacctc
     2101 gtcgcccggc agggtggcga ccaccttgta gagatcccag gggcccttgg actcctgcgg
     2161 ggtcttgacc tgcatgaggt acatgtcgtg gatcatgcgg ccgtcggcgc gcacgtagcc
     2221 gcccttggcg aagaagtcgt tgatcttgtg cgacttcatc cacttcatga cggtgtcggg
     2281 atcgtcggtg ccggtttcct tgacggcgtt caggtagaac atggtcgcgg aatagtcgcc
     2341 cgcctgcagc atggagggtt tgcggccgac cttggcctcg aacttcttgg cccaggcgcg
     2401 ggtgtcgtcg ttcaggtccc agtaccagcc gtcggtcagg tacatgccct tggtggcatc
     2461 caggcccagc gcgtgcacgt cgttgatgaa gaccagcatg ccggccagtt tcatggacgc
     2521 ggtgacgccg aattcgttgg cggccttgac cgagttgatg gtgtcgccgc ccgcgttggc
     2581 caggcccagg atctgcgcgc ccgaggactg cgcctgcagc atgaaggacg agaagtcggt
     2641 ggtgccgagc ggcgcgcgca ccgaaccctt gaccgtgccg ccggtggcct tgatcacggc
     2701 ggtcgtgtcg cgttcgagcg cctggccgaa ggcgtagtcg gccgtcagga agaaccagct
     2761 cttgccgccg tccttcacca cggccgaacc ggtgccgcgc gccagcgcga cggtgtcgta
     2821 ggcatagtgg atggtgtagg gcgagcactg ggtggtggtg agctcggaag cgccggcccc
     2881 gatggcgatg aagggcttgt gcttttccgc ggcgaccacg gccatggaca ggccggtgcc
     2941 cgagttggtg ccgccgatca gcatgtcgac tttttgctgg tcgaaccatt cgcgcgc
//
LOCUS       DASWPO010000242         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_415182, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000242 DASWPO010000000
VERSION     DASWPO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415182"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGC69_12630"
     CDS             <1..263
                     /locus_tag="VGC69_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY0296170.1"
                     /translation="AFEARFRDGAIPDDMPEISVAGAPVGILKLLRESGLAASSSEAQ
                     RNIEQGGVRIDGARVEDKSLQLSAGSYVLQVGKRKFARVNLA"
     gene            382..1035
                     /locus_tag="VGC69_12635"
     CDS             382..1035
                     /locus_tag="VGC69_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY0296171.1"
                     /translation="MKLSSLSFSDHESIPERYAFGKIDPQSHVALADNFNPQFSWDDV
                     PAGTRSFVLICHDPDVPSRPDDVNQEGRLVPADLPRVDFFHWLLVDLPADLREIDESA
                     FSSGITPRGKGGPLAPIMNARQGLNDYTSWFAGDHDMSGDYFGYDGPCPPWNDAIVHH
                     YVFTLYALDVETLPVQGSFKGVDVLSAMQGHVLAKDSVTGIYTLNPDLAPTQIATSE"
     regulatory      1057..1180
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            1249..2496
                     /gene="glyA"
                     /locus_tag="VGC69_12640"
     CDS             1249..2496
                     /gene="glyA"
                     /locus_tag="VGC69_12640"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY0296172.1"
                     /translation="MFDRNLTLDKVDPEVWAAVQQENTRQEQHIELIASENYASPAVM
                     QAQGTQLTNKYAEGYPGKRYYGGCEFVDIVETLAIERLKKIFVAEAANVQPNSGSQAN
                     QGVYNAMLKPGDTVLGMSLAEGGHLTHGASVNQSGKLYNFIQYGLDANEVLDYAKVEA
                     LAQEHKPKMIVAGASAYSLHIDFERLGKIARDNGAWLMVDIAHYAGLVAGGAYPNPVP
                     HADFVTSTTHKSLRGPRGGVIMMKAEFEKAVNSAIFPGIQGGPLMHVIAAKAVAFQEA
                     LAPEFKTYAQQVVKNARVLADTLVKRGLRIVSGKTESHVMLVDLRAKGITGKEAEAVL
                     GQAHITVNKNAIPNDPEKPFVTSGIRLGTPAMTTRGFTEAEAELTANLIADVLDNPRD
                     EANIAAVRERVNALTARLPVYRG"
BASE COUNT          490 a          941 c          774 g          386 t
ORIGIN      
        1 cggccttcga ggcgcgcttt cgcgacggcg ccatcccgga cgacatgccc gagatctcgg
       61 tggccggcgc gcccgtcggc atcctgaagc tcctgcgcga atccggcctg gcggcctcga
      121 gttccgaggc gcagcgcaat atcgaacagg gcggcgtgcg catcgacggc gcccgcgtcg
      181 aagacaaatc gttgcaattg tctgccggtt cttatgtgct ccaggtaggc aaacgtaaat
      241 ttgcccgtgt taacttggcc taaccggcaa gtgtgagccc cccggctgcc gcgcagccac
      301 tcccttgggg gcccggcccg gcttggggcg gcccggcgcc aggccgcccg ccctgaaaac
      361 cagccagcag gagatgacgt catgaagctc tcgagtcttt ccttttccga ccacgagtcc
      421 attcccgaac gctatgcctt cggcaagatc gatccccaat cgcacgtcgc attggccgac
      481 aacttcaacc cccagttctc ctgggacgac gtgccggcgg gcacccgctc gttcgtgttg
      541 atctgccacg accccgacgt gccctccagg cccgacgacg tcaaccagga aggccgcctc
      601 gtgccggccg acctgccgcg cgtggatttc ttccattggc tgctggtgga cctgccggcc
      661 gacctgcgcg agatcgacga aagcgccttc tccagcggca tcacgccgcg cggcaagggc
      721 ggcccgctgg cccccatcat gaacgcgcgc cagggcctca acgactacac cagctggttc
      781 gccggcgacc acgacatgag cggcgactac ttcggctacg acggcccctg cccgccgtgg
      841 aacgatgcca tcgtgcacca ctatgtcttc acgctctacg cgctcgacgt cgagacgctg
      901 ccggtgcagg gcagtttcaa gggcgtggac gtgctgtccg ccatgcaggg gcatgtgctg
      961 gccaaggact cggtcacggg catctacacc ctgaatcccg accttgcgcc cacccagatc
     1021 gccaccagcg aataaggtaa aatttgctcg attgcctttc cctcgcgtaa ctggcgaatc
     1081 cgtgggtcac cacaaggaag cgcgagggtc atcagcgccg ggggcggcgc atgcagcgcg
     1141 caccgcccgc cgacgtacgc cgttcgcctg ggcagacgtt aggcagatct tgtagcagca
     1201 gcaacgatcc ccattaacgc catccctgaa cgacaaggac ctctcgccat gttcgaccgc
     1261 aatctcaccc tcgacaaagt cgaccccgaa gtctgggccg ccgtccagca agaaaacacc
     1321 cggcaggaac agcacatcga gctgatcgcc tccgaaaact acgccagccc cgccgtcatg
     1381 caggcgcagg gcacccagct caccaacaag tacgccgaag gctacccggg caagcgctac
     1441 tacggcggct gcgaattcgt cgacatcgtc gagacgctgg ccatcgagcg cctgaagaag
     1501 atcttcgtcg ccgaagccgc caacgtgcag cccaattcgg gctcgcaggc caaccagggc
     1561 gtctacaacg ccatgctcaa gccgggcgac accgtgctgg gcatgagcct ggccgaaggc
     1621 ggccacctca cgcacggcgc ctcggtcaac cagtcgggca agctgtacaa cttcatccag
     1681 tacggcctgg acgccaacga agtgctcgac tatgccaagg tcgaggccct ggcacaagaa
     1741 cacaagccca agatgatcgt ggccggcgcc tcggcctact cgctgcacat cgacttcgag
     1801 cgcctgggca agatcgcgcg cgacaacggc gcctggctga tggtcgacat cgcccactac
     1861 gcgggcctgg tggccggcgg cgcctatccc aaccccgtgc cgcacgccga cttcgtcacc
     1921 tccaccacgc acaagtccct gcgcggcccg cgcggcggcg tcatcatgat gaaggccgag
     1981 ttcgagaagg ccgtcaattc ggccatcttt cccggcatcc agggcggtcc gctgatgcac
     2041 gtcatcgccg ccaaggccgt ggccttccag gaggccctgg cgcccgagtt caagacctac
     2101 gcgcagcaag tggtcaagaa cgcccgcgtc ctggccgaca cgctggtcaa gcgcggcctg
     2161 cgcatcgtct ccggcaagac cgaaagccac gtcatgctgg tcgacctgcg cgccaagggc
     2221 atcaccggca aggaagccga ggccgtgctg ggccaggccc acatcacggt caacaagaac
     2281 gccatcccca acgacccgga aaagcccttc gtcaccagcg gcatccgcct gggcaccccg
     2341 gccatgacca cccgcggctt caccgaggcc gaggccgagc tcaccgccaa cctgatcgcc
     2401 gacgtgctcg acaacccgcg cgacgaagcc aacatcgctg ccgtgcgcga acgcgtcaac
     2461 gccctgaccg cgcgcctgcc cgtttatcgc ggctaaagcg ggttgcctac tagtcaggcg
     2521 ggaagtggcg ggcatcgcgg gccctttgag gcgggggcag ccccgcaggg gccgagggcg
     2581 cctgatggcg c
//
LOCUS       DASWPO010000243        14115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2291624, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000243 DASWPO010000000
VERSION     DASWPO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291624"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VGC69_12645"
     CDS             complement(<1..541)
                     /locus_tag="VGC69_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0296173.1"
                     /translation="MQRIYDAVIVGGGPAGVSCAVWLARLGLSPLLVEAGERLGGLTA
                     TNPFVDDWIAALPGATGLQIADNLARSAQAAGVPVRLRAPAVQARAEADGFALTLAAS
                     QETVRGRALVIASGVLARNLPGHALRARWPGVLVGPGSSVVAQDYTGLSVAVLGGGDN
                     AFENFIYVRDHGAKTVHLYS"
     gene            605..964
                     /gene="folB"
                     /locus_tag="VGC69_12650"
     CDS             605..964
                     /gene="folB"
                     /locus_tag="VGC69_12650"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY0296174.1"
                     /translation="MLTRRIILSGLALDARVGILEHERRATQPLHIDAEFDVDITRKT
                     DDQDIHSVLDYRRLRETIIEQCTRAHVNLLETLTEQIAERLLADFPDVNAIRLRISKP
                     MAFSDCVAVGVEIQTSR"
     gene            1095..2039
                     /gene="ttcA"
                     /locus_tag="VGC69_12655"
     CDS             1095..2039
                     /gene="ttcA"
                     /locus_tag="VGC69_12655"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY0296175.1"
                     /translation="MDTTVFPTLAEQKARHEGNKLAKRLARETTRAISDYSMIEAGDR
                     VMVCLSGGKDSYGMLDILMALQKRAPFRFELIAVNLDQKQPGFPDDVLPRYLGGLGVP
                     FHIETQDTYSTVTRVIPEGKTLCSLCSRLRRGILYRVAGELGATKIALGHHRDDILGT
                     FFLNLFYGGKMKGMPPKLVSDDGRHTVIRPLAYVPESDLVAYAQLKQFPIIPCNLCGS
                     QENLKRREVSRMLQEWDRKHPGRAWNIFNALSSVVPSHLMDRELFDFAGLKPTGLPDA
                     DGDIAFDQEDPDGASPSCSDGDAAELDGMTEKAIRFVS"
     gene            complement(2207..3973)
                     /locus_tag="VGC69_12660"
     CDS             complement(2207..3973)
                     /locus_tag="VGC69_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0296176.1"
                     /translation="MGMNNSAILYRLQPFDPAGHRYRITLTVAQPAPEGQKLSLPAWI
                     PGSYLIRDFSRQVETLSARSRGRPVAVGKLDNHTWQAAPCDGPLEVDYTVYAWDLSVR
                     GAHVDETHAFFNGASVFLRVEGQAHEPCLLDLAAPPGLEHWKVYTALPEARGLRGAAR
                     RHGFGLYQAPDYDALIDYPVEMGTPQVIGFTAHGAPHELVFTGVIPRLDLQRIADDAR
                     AICETQIAFFEPRSKRAPFLDSTDRYVFMTTVTGDGYGGLEHRASTALLASRKDLPVI
                     GQQGQGEGYRTFLGLVSHEYFHTWNVKRIKPAAFVPYDLSQPGLTRLLWIFEGFTSYY
                     DDLLLLRSGAITRADYLRMLARTITGVARTPGRRKQSVAESSFDTWTRYYKQDENSLN
                     AIVSYYTKGALVALGLDLTIRQQSGGRHSLDDVMRLMWQRYGRDFYKGPQHGLDESVF
                     PALVREATGVDVRRFVARHAYGTADLPLAELLATAGVTLQWKPATPIPSMDVRVRKQG
                     ENLALATVLEGGAAHKAGLSAGDVLVAVDGLRVEAPAGLDQLVSQYRAGERVQVHVFR
                     RDELRAFTVRLGAPAALDCVLS"
     gene            4003..4779
                     /locus_tag="VGC69_12665"
     CDS             4003..4779
                     /locus_tag="VGC69_12665"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0296177.1"
                     /translation="MSEPLVLVETRGRVGLLTLNRPKALNALNDALMDQLGAALLAFE
                     ADADIGAIVITGSEKAFAAGADIGAMKDWSYMDVYGNEYITRNWETLKRIRKPVIAAV
                     AGYALGGGCELAMMCDILIAADTAKFGQPEIKLGVIPGAGGTQRLPRAIGKAKAMDLV
                     LTARMMGAEEAERAGLVSRLVPAGKLLDEAIEAATVIAAMSLPSAMLAKECVNRAYEG
                     SLAEGILFERRVFHSLFATEDQKEGMAAFVEKRAPAFKHR"
     gene            5060..5521
                     /locus_tag="VGC69_12670"
     CDS             5060..5521
                     /locus_tag="VGC69_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY0296178.1"
                     /translation="MRGDNTSIEAEEAEWQPVILSAEDRLVLDARACRDMLVMLAAQA
                     VMGLLAAALAGIWAGPAAGLSALLGAGAYWFPNAFFALRLLVNVVRSVRANPFAFLFG
                     ELTKLFLAALLMGLVAWYARGWLVWPAALAGLVFTLKGYLPFVLLRKLSRR"
     gene            5622..6503
                     /gene="atpB"
                     /locus_tag="VGC69_12675"
     CDS             5622..6503
                     /gene="atpB"
                     /locus_tag="VGC69_12675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0296179.1"
                     /translation="MAAESGVSPQAEYIQHHLVHLNNIGEKQPIIAQFNVVNYDSLFW
                     SILMGLLVVLGLWFAARRASSGVPGRFQTFVEMIVDLVDDQAKSIVNNAKSRLFVTPL
                     ALTVFLWIIFMNALDLLPVDLPHVIFHALGFGRQQGDFLYYHRIVPTADLNVPMGMSL
                     SVLLLMFYYGIKIKHPGGFVKELFTAPFHAHGVGAVILAPINLLMNVIEYAAKSVSLG
                     MRLFGNMFAGELLFLLIALLGGAWTGFNGTSIALGAGQIVAGSIWAIFHILIVLLQAF
                     IFMMLTLVYLGQAHEGH"
     gene            6614..6856
                     /gene="atpE"
                     /locus_tag="VGC69_12680"
     CDS             6614..6856
                     /gene="atpE"
                     /locus_tag="VGC69_12680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY0296180.1"
                     /translation="MTNVAFVALACGLIIGLGAIGACIGIGLMGGRYLEASARQPELM
                     NALQTKMFLLAGLIDAAFLIGVGIAMLFAFANPFAG"
     gene            7033..7503
                     /locus_tag="VGC69_12685"
     CDS             7033..7503
                     /locus_tag="VGC69_12685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY0296181.1"
                     /translation="MNLNATIFFQMIVFFVLGWFTMKFVWPPLTKAIDERRQKIADGL
                     AASEKGKADLAQAQARVSLMEASAKSDNHARMAEAEKQAAGLIEQARREAEAERARIV
                     AQAAQDAAQEVQRARDALRDDVAALAVKGAAQILKREVDARAHAELLDQLKAQL"
     gene            7597..8139
                     /locus_tag="VGC69_12690"
     CDS             7597..8139
                     /locus_tag="VGC69_12690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY0296182.1"
                     /translation="MAELSTVARPYAEALFEAAGDDQALGLPGWADLVGEMAQVAANP
                     DVREAMGDPRLDDAQRIQVFSSLLKSTLPASAGNFIELLVQNDRLLLLPEIAEQFIVL
                     KNRHEGTAQAEITSAFELTEAQVKDLVVALESKFGLKLKPHVTVDPSLIGGVRVAVGD
                     QVLDTSVQAQLTRMRDMLAA"
     gene            8186..9727
                     /gene="atpA"
                     /locus_tag="VGC69_12695"
     CDS             8186..9727
                     /gene="atpA"
                     /locus_tag="VGC69_12695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0296183.1"
                     /translation="MQLNPSEISELLKSRIEGLGASTDVRTQGTVVSVTDGITRIHGL
                     SDVMQGEMLEFPNNVYGVALNLERDSVGAVILGSYLGVSEGDQVKTTGRILDVPVGPE
                     LKGRVVNTLGVPIDGKGPINAKETDIIEKVAPGVIARRSVSQPLQTGIKAIDAMVPIG
                     RGQRELIIGDRQTGKTAVAVDAIISQKGKGVTCVYVAIGQKASTVNNVVRKLEEHGAM
                     EFTIVVVATASESAAMQYLAAYAGCTIGEYFRDRGEDALIVYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEDYVEKFTKGAVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGVRPAINAGISVSRVGGAAQTKVIKKLSGG
                     IRTDLAQYRELAAFAQFASDLDDATRRQLERGKRVVELLKQPQYQPLQVWELAVSLFA
                     VNSGYLDDIDVTQVLSFEKAFKEQLKSKHAGLIQRIEDTKELSKDDEAELTAAVQDFK
                     KHGAF"
     gene            9744..10652
                     /gene="atpG"
                     /locus_tag="VGC69_12700"
     CDS             9744..10652
                     /gene="atpG"
                     /locus_tag="VGC69_12700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY0296184.1"
                     /translation="MPGIKEIRTKIKSVQNTRKITKAMEMVAASKMRKAQDRMRAGRP
                     YARKVRDIAMHLMQANPEYSHPFLVEREMKAVGVVMVTTDKGLCGGLNTNISRVVLAK
                     LKEFQHKGIAVQTTALGNKGLGLLTRISSKLVSQEVQLGDKPDMERLLGAIKVQLDDY
                     LAGRIDALYLATTRFVNTMRQEPTFVRLLPLAGSLDDPFQSGVSDEQSEGAKLATSHG
                     WDYIYEPDAQSVIDDLLQRYIEGLLYQGVAENMASEQSARMVAMKAASDNAKKVISDL
                     QLVYNKTRQAAITKEISEIVGGAAAV"
     gene            10695..12098
                     /gene="atpD"
                     /locus_tag="VGC69_12705"
     CDS             10695..12098
                     /gene="atpD"
                     /locus_tag="VGC69_12705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0296185.1"
                     /translation="MSNGTIVQCIGAVVDIQFPRDHMPKIYEALTLVDEDASSFAEKG
                     LTLEVQQQLGDGVVRTIALGSSDGLRRGMQVAGTGAPISVPVGTGTLGRIMDVLGRPI
                     DDAGPIVADEKRGIHQAAPAFDELSPSVELLETGIKVIDLVCPFAKGGKVGLFGGAGV
                     GKTVNMMELINNIAKQHSGLSVFAGVGERTREGNDFYHEMEESNVLDKVAMVFGQMNE
                     PPGNRLRVALTGLTMAEKFRDEGLDILFFVDNIYRYTLAGTEVSALLGRLPSAVGYQP
                     TLAEEMGVLQERITSTKTGSITSIQAVYVPADDLTDPAPATTFQHLDSTVVLSRDIAA
                     LGIYPAVDPLDSSSRQLDPLVIGDEHYQVARGVQQTLQRYKELRDIIAILGMDELSPE
                     DKQAVARARKIQRFLSQPFHVAEVFTGSPGKYVPLAETIRGFKMIVNGECDSLPEQAF
                     YMVGTIDEAFEKAKKLQ"
     gene            12123..12548
                     /locus_tag="VGC69_12710"
     CDS             12123..12548
                     /locus_tag="VGC69_12710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY0296186.1"
                     /translation="MAILQVDVVSAQEAIFAGEAKFVVLPGESGELGILPGHTPLISR
                     IRPGTVKIVRPDNSEELVFVAGGILEVQPGSVTVLADTAIRGADLDEARAEEARKKAE
                     EALRNTKDKADIAAVEAELAMLTAEAAAARKIRQSRSTH"
     gene            12753..13523
                     /locus_tag="VGC69_12715"
     CDS             12753..13523
                     /locus_tag="VGC69_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0296187.1"
                     /translation="MREVFECALLQAPGLAESGDRLRLQVYDLGEAVRAGREGGAAPD
                     TTGLIASAALALQRYDACLLYVEPANLPWARIALARARPVLRTPVLALVRDVKAAALA
                     DLLALGVADFVRMPMCPEELRARLMRLAGRTLLREPGQAYGAAPSSAAAAAPRAARPR
                     LQRSGVNIALYDPDEPFRQAKARIVSGFERAYLCHALQRHEGNVAQAARASNKHRRAF
                     WALMHKHAIDARAYRSAVAGGHARPPACNRPRPRPAKR"
     gene            13565..>14115
                     /locus_tag="VGC69_12720"
     CDS             13565..>14115
                     /locus_tag="VGC69_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY0296188.1"
                     /translation="MPSVLKNDVFLRALLRQPVPYTPIWLMRQAGRYLPEYNATRARA
                     GSFLGLAQNPDYATEVTLQPLARYPLDAAILFSDILTVPDAMGLDLSFAQGEGPRFAR
                     PVRDEADVARLAVPDLDKLRYVFDAVASIRRELDGRVPLIGFAGSPWTIACYMVEGQG
                     STDYRLIKSMLYARPDLLHRILG"
BASE COUNT         2473 a         4769 c         4522 g         2351 t
ORIGIN      
        1 gcgagtacag atgcacggtt ttcgcgccat ggtcgcgcac gtagatgaaa ttctcgaagg
       61 cattgtcgcc gccgcccaga accgccaccg acaggccggt atagtcctgc gccacgacgg
      121 acgatccggg ccccaccaga acgccgggcc agcgggcccg cagcgcatgg cccggcagat
      181 tgcgcgccag cacgcctgag gcgatgacca gcgcgcggcc gcgcacggtt tcttgcgagg
      241 cggccagcgt cagggcaaag ccatcggcct cggcgcgcgc ctggacggcc ggcgcgcgca
      301 gccgcacagg cacgccggcg gcctgcgcgc tgcgcgccag gttgtcggcg atctgcagtc
      361 cggtggcccc gggcagagcg gcgatccagt cgtccacgaa agggttggtg gccgtcaggc
      421 cgcccagccg ttccccggcc tcgaccagca ggggcgaaag tcccaggcgc gccagccaga
      481 ccgcgcacga caccccggcc ggtccgcctc ccacgatcac cgcgtcgtat atccgttgca
      541 tgcccataga agctgattgt agtcacgctt tacaatgcct ccttcccacc caggtcccga
      601 catcatgctg acccgccgca tcattctttc cggtctggcg ctcgacgccc gtgtcggcat
      661 actcgagcac gaacgccgcg ccacgcagcc cctgcacatc gatgccgagt tcgacgtgga
      721 catcacgcgc aagaccgacg accaggacat ccacagcgtg ctggactacc ggcgcctgcg
      781 cgagaccatt atcgaacagt gcacccgggc gcatgtgaac ctgctcgaga cgctcaccga
      841 gcagatcgcc gaacgcctgc tggccgactt cccggacgtc aacgcgatca ggctgcgcat
      901 cagcaagccc atggcttttt cggattgcgt ggcggtcgga gtggagatcc aaaccagccg
      961 gtgatgtttg atttattggg tcaggtggtt ttttgagttc ttgagccgcc gcggctgccc
     1021 tgcggaattc cgatgctcgc ccccagcggg gctgtgcttc ggattcctcc ggggtccgcg
     1081 gctcacatat cgtcatggac actaccgttt ttcctacgct ggctgaacaa aaggcgcggc
     1141 acgaaggcaa caagctcgcc aagcgcctgg cgcgcgagac cacgcgcgcc atttccgact
     1201 acagcatgat cgaggcgggc gaccgcgtga tggtgtgcct gtcgggcggc aaggactcgt
     1261 acggcatgct cgacatcctg atggcgctgc aaaagcgcgc gccgttccgg ttcgagctca
     1321 tcgccgtcaa cctcgaccag aagcagccgg gctttcccga cgacgtgctg cccaggtacc
     1381 tgggcggcct gggcgtgccc ttccacatcg agacgcagga cacctattcc accgttacgc
     1441 gcgtcatccc cgaaggcaag accctgtgct cgctgtgctc gcggctgcgg cgcggcattt
     1501 tgtaccgcgt ggccggcgaa ctgggcgcca ccaagatcgc gctgggccac catcgcgacg
     1561 acatcctggg caccttcttc ctgaacctgt tctacggcgg caagatgaag ggcatgccgc
     1621 ccaagctggt gtccgacgac ggccggcaca cggtcatccg gccgctggcc tatgtgccgg
     1681 aaagcgacct ggtcgcctac gcccagctca agcagtttcc catcatcccc tgcaacctgt
     1741 gcggttcgca ggaaaacctc aagcgccgcg aagtctcgcg catgctgcaa gagtgggacc
     1801 gcaagcaccc gggccgcgcc tggaacatct tcaacgcgct ctcgagcgtg gtgccttcgc
     1861 acctgatgga ccgcgagctg ttcgacttcg cgggcctcaa gcccacgggc ctgcccgatg
     1921 ccgacggcga catcgccttc gaccaggaag acccggacgg cgcatcgcca tcgtgcagcg
     1981 atggcgatgc cgccgagctt gacggcatga cagaaaaagc gatccggttc gtttcgtagc
     2041 cggcattgag ttgccgtgag ccgaggaccc cggaggaatc cgaagcgcag cccccactgg
     2101 gggcgagcat cggaattgcg cagggcagcc tcggcgcctc aagaactcag atcgccacgg
     2161 cccaagaact caaatcgccg cagtccgaga accccaaaac ggcaactcaa gacaacacgc
     2221 aatccaaggc cgccggcgcc cccagcctca cggtaaacgc ccgcagctca tcccgccgga
     2281 acacgtgcac ctgcacgcgc tcccccgccc ggtattgcga caccaactgg tccagcccgg
     2341 ccggcgcctc cacgcgcagg ccatcgacgg ccaccagcac gtctcccgcc gacagccccg
     2401 ccttgtgcgc ggcgccgcct tccagcaccg tggccagcgc cagattctcg ccctgcttgc
     2461 gcacccgcac gtccatggaa ggaatcggcg tggccggctt ccattgcagc gtcacgccgg
     2521 ccgtcgccag caattcggcc agcggcagat cggccgtgcc gtaggcatgg cgcgccacga
     2581 agcgccgcac gtccacgccc gtggcctcgc gcaccagcgc gggaaagacc gactcgtcca
     2641 ggccgtgctg cgggcccttg tagaaatccc ggccgtagcg ctgccacatc aggcgcatca
     2701 cgtcgtccag cgaatgccgg ccgccgctct gctggcggat ggtcaggtcc aatcccagcg
     2761 ccaccagcgc gcccttggtg tagtagctga cgatcgcgtt gagcgagttc tcgtcctgct
     2821 tgtagtagcg cgtccaggtg tcgaacgagc tttcggccac cgactgcttg cgccggccgg
     2881 gcgtgcgcgc caccccggtg atggtcctgg ccagcatgcg caggtaatcg gcgcgggtaa
     2941 tcgcgcccga gcgcagcagc agcaagtcgt cgtagtacga ggtgaaaccc tcgaaaatcc
     3001 acaagaggcg cgtcaggccg ggttgcgaca gatcgtaggg cacgaaggcg gccggcttga
     3061 tgcgcttgac gttccaggta tggaagtact cgtggctcac caggcccagg aaggtgcggt
     3121 agccctcgcc ctggccctgc tggccgatca cgggcaggtc cttgcgcgag gccagcagcg
     3181 ccgtgctggc gcggtgttcc aggccgccgt agccgtcgcc cgtgaccgtg gtcatgaaca
     3241 cgtagcggtc ggtgctgtcg agaaagggcg cgcgcttgct gcgcggctcg aaaaaggcga
     3301 tctgggtttc gcagatggcg cgcgcgtcgt cggcgatgcg ctgcaggtcc agcctgggga
     3361 tcacgccggt gaacaccagc tcgtgcggcg cgccgtgcgc cgtgaagccg atcacctgcg
     3421 gcgtgcccat ttccaccgga tagtcgatca gcgcgtcgta gtcgggcgcc tggtacaggc
     3481 cgaagccgtg ccgccgcgcc gcgccgcgca ggccacgggc ctcgggcagg gcggtgtaga
     3541 ccttccagtg ctccagcccg ggcggggcgg cgaggtcgag caggcagggt tcgtgcgcct
     3601 ggccttcgac gcgcaggaac acgctggcgc cgttgaagaa ggcgtgggtc tcgtccacgt
     3661 gcgcgccgcg cacggacagg tcccacgcgt agacggtgta gtcgacttcg agcgggccgt
     3721 cgcagggggc ggcctgccag gtgtggttgt cgagtttgcc cacggccacc gggcggccgc
     3781 ggctcctggc cgagagcgtc tcgacctggc gcgagaagtc gcggatcagg tagctgccgg
     3841 gtatccaggc gggcagggag agtttctgtc cttcgggggc gggctgggcc acggtgagcg
     3901 tgatgcggta ccggtggccg gcgggatcga agggctgcag gcggtaaaga atggcgctat
     3961 tgttcatgcc tatatcttat tcccaccaca ggagacatac tcatgagcga accccttgtt
     4021 ctggtcgaga cccgtggccg ggtgggcctc ttgaccctga accgtcccaa ggccctgaac
     4081 gcgctgaacg acgccctgat ggaccagctc ggcgcggccc tgctggcctt cgaggccgac
     4141 gcggacatcg gcgccatcgt catcaccggc agcgaaaagg cctttgccgc gggcgccgac
     4201 atcggcgcca tgaaagactg gtcgtacatg gacgtctacg gcaacgagta catcacccgc
     4261 aactgggaga cgctcaagcg catccgcaag ccggtgatcg ccgcggtggc gggctatgcc
     4321 ctgggcggcg gctgcgagct ggccatgatg tgcgacatcc tcatcgccgc cgacacggcc
     4381 aagttcggcc agcccgagat caagctgggg gtcatccccg gggccggcgg cacgcagcgc
     4441 ctgccgcgcg cgatcggcaa ggccaaggcc atggacctgg tcctgacggc gcgcatgatg
     4501 ggcgccgagg aagccgaacg cgccggcctg gtgtcgcgcc tggtgccggc cggcaagctg
     4561 ctcgacgagg ccatcgaggc cgccacggtc atcgccgcca tgtcgctgcc ctcggccatg
     4621 ctggccaagg aatgcgtcaa tcgcgcctac gaagggtcgc tcgccgaagg catcctgttc
     4681 gagcgccggg tgttccattc gctcttcgcc accgaagacc agaaagaggg catggccgcc
     4741 ttcgtggaga agcgcgcgcc ggcgttcaag caccgttgaa tcccgctttg ccgacgtctt
     4801 tcccgggggc tcccggacgg ctgacagcgg gcttgctaat ttgcggtttt taaacaaagc
     4861 aagctatact tgatgggttt gatgcttagc gaaacacggt tttcgcgtgc gagttgcaaa
     4921 ttgatacgag gtggggccaa gcgatctggt gccacctttt gttgtctaat ttgcagccta
     4981 aacctaaaag ggcctgatcg attcgggccc aacaggaagg attcagggct gaaaagcccg
     5041 aggcgcgcga gtcgtggcaa tgcggggaga caacaccagt atcgaagcgg aagaggcgga
     5101 atggcagccg gtaatcttgt cggccgaaga tcgcctggtg ctcgatgctc gggcttgccg
     5161 ggacatgctg gtcatgttgg cggcccaggc ggtgatgggg cttctcgctg cggcgctggc
     5221 ggggatatgg gcggggccgg cggcggggct gtcggcattg ctgggggcgg gggcctattg
     5281 gtttcccaat gcgttttttg cgctgcgcct tctggtcaat gtcgtgcggt cggtgcgggc
     5341 gaaccccttt gcctttctct tcggtgagtt gaccaagctg tttctagccg cgctgctgat
     5401 gggccttgtc gcctggtatg cgcggggatg gctggtctgg ccggcggcgc tggccggcct
     5461 ggtgttcacg ctgaagggtt atctcccgtt cgtgttgttg cgcaagctgt caaggcggta
     5521 ggcggtatcc agaacataaa tgctcggctg ttcatgccag gctcctcgcg gggctacagg
     5581 gacggcttgt ccagtaaacg gcagtaaaag gtagggttct catggctgca gaaagcggtg
     5641 tctcgcctca ggcagagtac attcagcacc acttggtgca cctgaataat atcggcgaaa
     5701 agcagcccat catcgcccag ttcaacgtcg tcaactacga ctcgctgttc tggtccatcc
     5761 tcatgggtct cctggtggtg ctgggcctgt ggttcgccgc ccgccgcgcg tcttccggcg
     5821 ttcccggccg cttccagacc ttcgtggaaa tgatcgtcga cctggtcgac gaccaggcca
     5881 agagcatcgt gaacaacgcc aagagccgcc tgttcgtgac cccgctggcg ctcaccgtct
     5941 tcctctggat catcttcatg aacgcgctcg acctgctgcc ggtcgacctg cctcacgtga
     6001 tcttccatgc cctgggcttc ggccggcaac agggtgattt cctgtactac caccgcatcg
     6061 ttcccacggc cgacctgaac gtgcccatgg gcatgtcgct ctccgtgctg ctgctgatgt
     6121 tctattacgg catcaagatc aagcacccgg gcggcttcgt caaggaactg ttcaccgcgc
     6181 ccttccatgc gcatggtgtc ggcgccgtga tcctggcgcc catcaatctg ctgatgaacg
     6241 tcatcgaata cgccgccaag tccgtgtcgc tgggcatgcg gttgttcggc aacatgttcg
     6301 ccggcgagct gttgttcctg ctgatcgccc tgctgggcgg cgcctggacg ggtttcaacg
     6361 gcacgagcat cgccctgggc gcgggccaga tcgtggccgg ctccatctgg gcgatcttcc
     6421 acatcctgat cgtgctgctg caggccttca tcttcatgat gctgacgctg gtgtatctcg
     6481 gccaggccca cgaaggacac taatcccctt tttccggcgc gggccgcgcg gcccgcgccg
     6541 gatcgcaaga gtttcttgca ttgagcagta cccattttca aatctgaact cagatattta
     6601 acaaggagtt gtcatgacca acgtcgcttt cgttgccctc gcttgcggtc ttatcatcgg
     6661 tctgggcgcc atcggcgcct gcatcggcat tggcctgatg ggcggtcgct acctggaagc
     6721 ctcggcccgc cagcccgaac tgatgaacgc cctgcagacc aagatgttcc tgctggccgg
     6781 cctgatcgac gctgcgttcc tgatcggcgt gggtatcgcc atgctgttcg ccttcgccaa
     6841 ccccttcgcg ggctgagcca ttggtcgatg gcaacgcccg ccgccgaggc aatatgtcca
     6901 gggggcgggt tttgacgccg ggggctgggg gcttgtccca atggggacca ggctctttcc
     6961 cgccggcaca gtatcgggag ctccggaacg cgatggtcgt ggccggagac gcaaggtgtt
     7021 aaaggaacga ccgtgaatct gaacgcgacg atctttttcc agatgatcgt gttcttcgtt
     7081 ctgggttggt tcacgatgaa attcgtgtgg ccccccctga cgaaggcaat cgacgagcgc
     7141 cgtcagaaaa tcgctgacgg actggctgcc tctgaaaaag gcaaggccga tctggcccag
     7201 gcccaggcgc gtgtcagcct gatggaagcg tctgccaagt ctgacaatca tgcccgcatg
     7261 gccgaggccg agaagcaggc tgccggactg atcgagcagg cccgccgcga ggcggaggcg
     7321 gaacgtgccc gcatcgtggc ccaggccgcg caggacgccg cccaggaagt gcagcgcgcc
     7381 cgcgatgcgc tgcgcgacga cgtggcggcg ctggccgtca agggcgccgc gcagatcctc
     7441 aagcgcgaag tcgacgcacg tgcccacgcc gagctgctcg atcagctcaa ggcgcagctt
     7501 tgatgcgagc cgagatgacg acccccacgc tcgctattcg ttcgctgccc cccgaggggg
     7561 cttcggcctc cttcgagcgg ccgtgcggag tccgacatgg ctgaactttc cactgtcgcc
     7621 cgcccctacg ccgaggccct gttcgaagcg gccggcgacg accaggccct gggcctgccg
     7681 ggctgggccg atctggtcgg cgaaatggcg caggtcgccg cgaaccccga cgtgcgcgaa
     7741 gccatgggcg acccgcgcct ggacgacgcg cagcgcatcc aggtcttcag cagcctgctc
     7801 aagagcacgc tgcccgcatc ggccggcaac ttcatcgagc tgctggtgca gaacgatcgc
     7861 ctgctactgc tgcccgagat cgccgagcaa ttcattgtgc tgaagaatcg ccatgaaggc
     7921 acggcgcaag cggaaatcac cagcgcgttc gaactcaccg aagcccaggt caaggacctg
     7981 gtcgtcgcct tggaaagcaa gttcggcctc aagctcaagc ctcacgtcac ggtcgatccc
     8041 tcgctgatcg gcggtgtgcg cgtggccgtg ggcgaccagg tgctcgacac ttccgtccaa
     8101 gcccaactga cccgcatgcg cgacatgctg gccgcgtagt ttccaaaacc acggccagcc
     8161 cctcacagga ttccaggagt caacaatgca actcaatccc tccgagatca gcgaactgct
     8221 caagagccgc atcgagggcc tgggcgcttc gaccgatgtt cgtacgcaag gcaccgtcgt
     8281 gtccgtgacc gacggcatca cccgcatcca cggcctgtcc gacgtgatgc agggcgaaat
     8341 gctcgaattc cccaacaacg tctacggcgt ggcgctgaac ctcgagcgcg actcggtcgg
     8401 cgccgtgatc ctgggcagct acctcggcgt gtccgaaggc gaccaggtca agaccaccgg
     8461 ccgcattctc gacgtgcccg tcggccccga actcaagggc cgcgtggtga acacgctggg
     8521 cgtgcccatc gacggcaagg gccccatcaa cgccaaagaa accgacatca tcgaaaaagt
     8581 ggccccgggc gtgatcgccc gccgctcggt gtcgcaaccg ctgcaaaccg gcatcaaggc
     8641 catcgacgcc atggtgccga tcggccgcgg ccagcgcgag ctgatcatcg gcgaccgcca
     8701 gaccggcaag accgccgtgg ccgtcgacgc catcatcagc cagaagggca agggcgtcac
     8761 ctgcgtgtac gtcgccatcg gccagaaggc gtccaccgtc aacaacgtgg tgcgcaagct
     8821 cgaagagcac ggcgccatgg aattcaccat cgtcgtggtc gccacggcct ccgagtcggc
     8881 cgccatgcag tacctggccg cctacgccgg ctgcacgatt ggcgaatact tccgcgatcg
     8941 cggcgaagac gccctgatcg tctacgacga cctgaccaag caagcctggg cctatcgcca
     9001 ggtgtcgctg ctgctgcgcc gcccgcccgg ccgcgaagcc taccccggcg acgtgttcta
     9061 cctgcactcg cgcctactcg agcgcgccgc ccgcgtcaac gaagactacg tcgagaagtt
     9121 caccaagggc gccgtgaagg gcaagaccgg ttcgctgacc gcgctgccca tcatcgaaac
     9181 ccaggcgggc gacgtgtcgg ccttcgtgcc gaccaacgtg atctcgatca ccgacggcca
     9241 gatcttcctg gaaaccgacc tgttcaacgc cggcgtgcgt cccgccatca acgccggtat
     9301 ctcggtgtcg cgcgtgggcg gcgccgccca gaccaaggtc atcaagaagc tgtccggcgg
     9361 tatccgtacc gacctggccc agtaccgcga actggccgcc ttcgcgcagt tcgcctccga
     9421 tctcgacgac gccacccgcc gccagctcga gcgcggcaag cgcgtggtgg agctgctcaa
     9481 gcagccccag taccagccgc tgcaggtgtg ggaactggcc gtgtcgctgt tcgccgtcaa
     9541 cagcggctac ctggacgaca tcgacgtcac ccaggtgctg tctttcgaaa aggcgttcaa
     9601 ggaacagctc aagtccaagc acgccggcct gatccagcgc atcgaagaca ccaaggaact
     9661 gtccaaggac gacgaagccg agctcaccgc ggccgtccag gatttcaaga agcacggtgc
     9721 tttttaagta acgggaaagc gcaatgcccg gaatcaagga aatccgcacc aagatcaaga
     9781 gcgtgcaaaa cacgcgcaag atcaccaagg cgatggaaat ggtcgccgca tccaagatgc
     9841 gcaaggcgca ggatcggatg cgcgcgggcc gtccctatgc ccgcaaggtg cgcgacatcg
     9901 ccatgcacct gatgcaggcc aacccggaat acagccaccc cttcctcgtc gaacgcgaga
     9961 tgaaggcggt gggtgtcgtc atggtcacta ccgacaaggg tctgtgcggc ggcctgaaca
    10021 ccaacatcag ccgcgtggtg ctggccaagc tcaaggagtt ccagcacaag ggcattgcgg
    10081 tgcagaccac ggccctgggc aacaagggcc tgggcctgct gacccgcatc agcagcaagc
    10141 tggtgtcgca ggaagtccag ctgggcgaca agcccgacat ggaacgcctg ctgggcgcca
    10201 tcaaggtgca gctcgacgac tacctcgccg gccgcatcga tgcgctctac ctggccacca
    10261 cgcgcttcgt caacacgatg cgccaggagc cgaccttcgt gcgcctgctg cccctggccg
    10321 gcagcctgga cgatcccttc cagagcggcg tgtccgacga gcagtcggaa ggcgccaagc
    10381 ttgccacgtc gcatggctgg gactacatct acgaacccga tgcgcagagc gtgatcgacg
    10441 atctgctgca gcgctacatc gaaggcctgc tgtaccaggg ggtggccgag aacatggcgt
    10501 ccgagcaatc ggcccgcatg gtggccatga aggcggcgtc ggacaacgcc aagaaggtca
    10561 tcagcgacct gcagctggtc tacaacaaga cccgccaggc ggcgatcacc aaggaaattt
    10621 cggaaatcgt ggggggcgcg gcggccgtct aaggcccccg ccacctgtca aacgctatca
    10681 agcaaggaat cgacatgagc aacggaacca tcgttcagtg catcggcgcc gtggtggata
    10741 ttcaattccc ccgcgatcac atgcccaaga tctacgaagc cctcaccctg gtcgacgagg
    10801 acgcctcctc gttcgccgag aagggcctca cgctggaagt ccagcagcag ctgggcgacg
    10861 gcgtggtgcg caccatcgcg ctgggttctt ccgacggcct gcgccgcggc atgcaggtcg
    10921 ccggcaccgg cgcgccgatc tcggtgcccg tgggcaccgg cacgctgggc cgcatcatgg
    10981 acgtgctggg ccgtcccatc gacgacgccg gccccattgt tgccgatgaa aagcgcggca
    11041 tccaccaggc cgcgcctgcc ttcgacgaac tgtcgccgtc ggtcgagctg ctggaaaccg
    11101 gcatcaaggt catcgacctg gtctgcccct tcgccaaggg cggcaaggtg ggcctgttcg
    11161 gcggcgccgg cgtgggcaag accgtcaaca tgatggaact gatcaacaac atcgccaagc
    11221 agcacagcgg cttgtcggtg ttcgccggcg tgggcgagcg cacccgcgaa ggcaacgact
    11281 tctaccacga aatggaagag tcgaacgtgc tggacaaggt cgccatggtg ttcggccaga
    11341 tgaacgaacc cccgggcaac cgcctgcgcg tggcgctgac cggcctgacc atggccgaga
    11401 agttccgcga cgaaggcctc gacatcctgt tcttcgtcga caacatctac cgctacaccc
    11461 tggccggcac cgaagtgtcc gccctgctgg gccgcctgcc ttccgccgtg ggctaccagc
    11521 ccaccctggc cgaagaaatg ggcgtgctgc aagagcgcat cacctcgacc aagaccggtt
    11581 cgatcacctc gatccaggcc gtgtacgtgc ctgccgatga cttgaccgac cccgcgcccg
    11641 ccacgacctt ccagcatttg gactcgaccg tcgtgctgtc gcgcgacatc gccgcgctgg
    11701 gcatctaccc cgccgtggat ccgctggact cctccagccg ccagctcgac ccgctcgtca
    11761 tcggcgacga gcactaccag gtggcccgcg gcgtgcagca gaccctgcag cgctacaagg
    11821 aactgcgcga catcatcgcc atcctgggca tggacgaact ctcgcccgaa gacaagcagg
    11881 ccgtggcccg cgcccgcaag atccagcgct tcctgtcgca gcctttccac gtggccgaag
    11941 tgttcaccgg ctcgcccggc aagtacgtgc cgctggccga gacgatccgc ggcttcaaga
    12001 tgatcgtcaa cggcgagtgc gattcgctgc ccgagcaggc cttctacatg gtcggtacca
    12061 tcgacgaggc cttcgagaag gccaagaagc tgcaataagc gtagttacaa ggaatacagg
    12121 gcatggctat ccttcaagtg gacgtcgtta gtgcgcagga agcgatcttc gccggcgagg
    12181 cgaagttcgt ggtgctgccg ggcgagtcgg gcgagctggg catcctgccc ggccacaccc
    12241 cgctgatctc gcgcatccgt cccggcaccg tcaagatcgt ccgtcccgac aacagcgaag
    12301 agctcgtctt cgtggcgggc ggcattctcg aagtgcagcc gggcagcgtg acagtcctgg
    12361 ccgacaccgc catccgcggc gccgacctgg acgaagcccg ggccgaggaa gcgcgcaaga
    12421 aagccgaaga ggccttgcgc aacaccaagg acaaggccga catcgccgcg gtcgaggccg
    12481 agctggccat gctgacggcg gaagccgccg ctgcgcgcaa gatccgccag agccgttcga
    12541 cgcattgacc gcgggcctgc gggcttcccg tgccgcaccc gatgcggcca cgcaaggcgg
    12601 gcttcggccc gccttgttca tttccggcgc atgtcgaagc cggtacctgt cagttttcgc
    12661 agcgtatatc ccgcagtgcg ggaagtgcga ctagtcgcgt ccggacaggc cacctaagct
    12721 ggctcctgcc ttgtttctct caggagcttt ccatgcgtga ggtgttcgaa tgcgcgttgc
    12781 tgcaggcgcc cggcctggcc gagagcggcg atcggctcag gctgcaggtc tacgatctgg
    12841 gcgaggccgt ccgcgcgggc agggagggcg gcgccgcgcc ggataccacg ggcctgatcg
    12901 cgtcggcggc cctggcgctg cagcgctacg atgcctgcct gctttatgtc gagccggcca
    12961 acctgccgtg ggcgcgcatt gcgctggcac gcgcccggcc tgtgctgcgc acgccggtgc
    13021 tggcgctggt gcgcgacgtc aaggccgcgg cgctggccga cctgctggcg ctgggcgtgg
    13081 cggatttcgt gcgcatgccc atgtgtcccg aggaactgcg cgcccggctc atgcgcctgg
    13141 ccggccggac gctgctgcgg gagcccgggc aggcctacgg cgccgcgccg tcctccgcgg
    13201 ccgcggcggc tccccgcgcg gcgcggccgc gcctgcagcg ttctggcgtc aacatcgctc
    13261 tgtacgatcc cgacgagccc tttcggcagg ccaaggcgcg catcgtgtcg gggttcgagc
    13321 gggcttatct gtgccatgcc ctgcagcggc acgaaggcaa tgtggcgcag gcggcccggg
    13381 catcgaacaa gcaccggcgg gccttctggg cgctcatgca caagcatgcc atcgatgcca
    13441 gggcctatcg ttcggcggtc gccggcgggc atgccaggcc gcccgcctgc aaccgtccgc
    13501 ggcccaggcc ggccaagcga tagcgcggca gtaaaatcac ggcttccgac cgacaaggat
    13561 agccgtgccc tccgtcctga agaacgatgt gttcttgcgt gccctgctgc gccagcctgt
    13621 gccctatacg cccatctggc tgatgcgcca ggcgggccgc tacttgcccg agtacaacgc
    13681 gacccgggcg cgcgcgggct cgttcctggg gctggcgcaa aaccccgact atgcaaccga
    13741 ggtgacgctg cagcccctgg cccgctatcc gctggacgcc gccatcctgt tttccgacat
    13801 cctgaccgtg cccgatgcca tgggcctgga cctgagcttc gcccagggcg aggggccgcg
    13861 ctttgcccgg cccgtgcgcg acgaggccga tgtggcccgc cttgccgtgc ccgatctcga
    13921 caagctgcgc tatgtgttcg atgcggtcgc gtcgatccgg cgcgagctcg atggccgcgt
    13981 ccccctgatc ggttttgccg gcagcccctg gaccatcgcc tgctatatgg tcgaagggca
    14041 gggcagcacc gactatcgcc tcatcaagag catgctctat gcgcgccccg atctgctgca
    14101 ccgcattctg ggcat
//
LOCUS       DASWPO010000244        18034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1041260, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000244 DASWPO010000000
VERSION     DASWPO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 18034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18034
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041260"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1739)
                     /locus_tag="VGC69_12725"
     CDS             complement(198..1739)
                     /locus_tag="VGC69_12725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0296189.1"
                     /translation="MVQIYCGFKRLVLLSISRLLTPRGVLATGAALWVGVAALCCWLV
                     YLAYQDAIRLATESSKNLSLAIQRDLERTIRSYDLSLRAVINGAGDPEIMSLPARLRN
                     RVLFDHSATAPYFGTIVLLDANGKVLADSAAASRTPVADFSDRKSYQVQRQDPALGLW
                     ISPPHRSKRRPDETEITVSRRVSSADGSFAGVVVGTIDVAYFHSLLDGIDLNRFGSAA
                     IIMTDGSLLARIPHVPSLVGRNFNTGAVFRKLTQEPSGVVWGTAGLDNVRRLYVFKRL
                     ETFPLILSVAPSALDILAEWRKRSLLISVLGALFSLVVLPASFLFARELRQRRKAEIA
                     LQRQADLDALTELENRGAFDRAIRNACARASRTGTALSLLFLDLDKFKVYNDSYGHQA
                     GDQALRRVAAAANAELQRLSDHFARYGGEEFVAILEGTRPEQAMRVAERIRAATERLG
                     IPHSGSPSGVVTVSIGVAGTQGEASPEALIRMADQALYDAKEQGRNCVAMFSPSEQTA
                     HAPGA"
     gene            complement(1832..4147)
                     /locus_tag="VGC69_12730"
     CDS             complement(1832..4147)
                     /locus_tag="VGC69_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0296190.1"
                     /translation="MQLLDKLNPEQRAAVTLEPQHALVLAGAGSGKTRVLTTRMAWLI
                     QTGQASPFSLLAVTFTNKAAREMLSRMTAILPIDTRGLWIGTFHGLCNRMLRAHYRDA
                     GLPQSFQILDTADQLAAIKRLLKAHNVDDEKYPPRDVQRFINNAKEGGERPGQMQAWD
                     AHSRRLVELYQLYEDQCQREGVVDFAELLLRAYELLSRNAPVREHYQRRFRHILVDEF
                     QDTNILQYKWLQLLAGGGAHVFAVGDDDQSIYAFRGAHVGNMADFERDYARGTVIRLE
                     QNYRSYGHILDSANALIGHNSGRLGKNLWTDQGEGEPVRVIEQPSDAMEAQWLVDEVR
                     ALVADGRLRREIAVLYRSNAQSRVIEHNLFSAGIPYKVYGGLRFFERQEIKHALAYLR
                     LMANPHDDTSWMRVVNFPTRGIGARTLEQLADAARTHGTSLYGAVAMVAGKGGSNLAQ
                     FAQLITRLAQETRELPLTELVGHMLDGSGLIAHYKTEREGAERLENLNELITAAAVFA
                     NEENYDGLPAGIAPPPAADEAGEFPPLVDADGVVAEGMTPLAAFLSHAALEAGDHQAE
                     AGQDAVQLMTVHAAKGLEFDAVFITGLEEGLFPHENSILEQSGLEEERRLMYVAITRA
                     RERLYLSLAQSRMLHGQTRYAMRSRFLDEIPEQHLKWITPKAGLARGGSWGDDFNNGR
                     GGASGNWRGDAFGRAHASNVAPRAARTMSSGVMVGEVQYRIGQGVRHARFGDGTIIGL
                     SGTGQDAQAQIQFRDVGQKTLALGIAKLEII"
     gene            complement(4287..5477)
                     /locus_tag="VGC69_12735"
     CDS             complement(4287..5477)
                     /locus_tag="VGC69_12735"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0296191.1"
                     /translation="MDLILSFLAGGLTQAHWWQVVVYTLVMTHITIVSVTVFLHRSQA
                     HRGLDLHPAVMHFFRFWLWMTTGMVTKEWVAIHRKHHAKCEKEGDPHSPILFGIWKVL
                     FRGADLYRVEAANQETLAKFGHGTPNDRLEQNLYAKHSLTGVLILLAIDIALFGAIGL
                     TVWAVQMAWIPFWAAGVVNGIGHYWGYRNYNSPDTSTNVIPWGFIIGGEELHNNHHAH
                     GTSAKFSAKWFEIDVGWGYISVLRMLGLAKIKKVAPKLKLDPTARAIDLRTLQGVITH
                     RYEVMARYTDVIRQAAREELSKLQGQSDRWAKLYRVRGSLTRNEEMLQPDQLAEVDST
                     IAQHPMLSTLVQMRRELGRLWESSSSSSEQLLVELQAWCHRAQQSGIAGLEQFAKRLS
                     RYAA"
     gene            5733..6155
                     /locus_tag="VGC69_12740"
     CDS             5733..6155
                     /locus_tag="VGC69_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296192.1"
                     /translation="MLNIARRLLAAAALAAAAPALADDIPPLAPGQQMVLANYYELSA
                     TGCRALNPPRVVITQQPALGKLIVVRTRGTANTSSPRCAVSTMSLPISQVLYQADKPG
                     LDTMAWAIKFQARVGPRGKDPRIEYGNARLQIKPGPAR"
     gene            complement(6133..7458)
                     /locus_tag="VGC69_12745"
                     /pseudo
     CDS             complement(6133..7458)
                     /locus_tag="VGC69_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220233.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
     gene            complement(7638..8237)
                     /gene="purN"
                     /locus_tag="VGC69_12750"
     CDS             complement(7638..8237)
                     /gene="purN"
                     /locus_tag="VGC69_12750"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY0296193.1"
                     /translation="MQALVEACQRDSWPARIAAVIASRPDAPGLDWARGQGIAARGIS
                     HKDYPSREAFDAALAEEIDRHRPDYVVLAGFMRVLTAGFVARYAGRLINIHPSLLPAF
                     PGLHTHAQALATGVRVHGCTVHFVTPVLDHGPIIAQGCVPVLAGDTPELLAERVLQVE
                     HQVFPAAVRWLAQGRVTLAADQRVDVAGDPQRLFMLEPS"
     gene            8399..9364
                     /locus_tag="VGC69_12755"
     CDS             8399..9364
                     /locus_tag="VGC69_12755"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY0296194.1"
                     /translation="MKPMPRIFRHTASAAPRPACALTIGNFDGVHLGHQAILASVRRE
                     SQVRGLVPAVMTFEPHPREYFAALYQRPELAPTRISGLRDKLSALVQAGIEEIFVQRF
                     DARLAAMSANSFIEDLLVRDMNVRWLLVGEDFRFGHKRSGDIELLREAGLAHGFEVRT
                     LADVADAHGHRISSSEVRTALAVGDLPRAQHLLGHPYRLSGHIIHGQKLGRQLGFPTM
                     NLRVAPRCAARSGIYVVRVLGLGDAPLPGVASLGVRPTVEDGGRVLLEAHVLDARVDA
                     YGKLVRIEFLRHLRDEEKFSDLPALTAAIAEDARNARAYFAAHGL"
     gene            complement(9414..10370)
                     /locus_tag="VGC69_12760"
     CDS             complement(9414..10370)
                     /locus_tag="VGC69_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296195.1"
                     /translation="MPARNSDPIEQFFRSGLKLSHLRMLVSLAELGQVTRVAAAFHVT
                     QPAISKQIAEIESALDVQIVQRLGNAVGLTGIGRVLVEHGREILRHIELARRDVSALA
                     AGTGGHVRVGAVVTIPEPIIASAVRLFMRRAPSSSFSFVEATLDRLIKLLDDGELDIA
                     LGRNHLTGVEMRLRQECLYREPFVFVAGVGHALGNPERPVSWEDLRPYRWVVPLRSSP
                     AFMSLAETLGAHDVALAGSAVESSSLALNVTLIESGDFVSILPLSVARGHMTRGTMRL
                     LPLPPLEALGEVVAYWRTDSTEPATSLFHECLRETSAEHFNE"
     gene            10588..11379
                     /locus_tag="VGC69_12765"
     CDS             10588..11379
                     /locus_tag="VGC69_12765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296196.1"
                     /translation="MTHSKQPVCMITGAATGIGAACARRFARAGWAVAIGNFDDGTRA
                     AALATEAACHGDGAPALVFDADVGANDDCRRAVDTVATAWGRIDALINCAGTTRFVPH
                     ADLAALDDAEFERVYRVNLIGMFQMTRAAESLLRETATAGRSASVINISSLAGLNGTG
                     SSIAYAASKGAANTLTLSLARSLAPHIRVNAIAPGMVDDGLLNRVLDAAAYERVVARM
                     IEAAPLKRVSQPAEVAELAWFIAAHAPALTGQVIAMENGLMLAGG"
     gene            11418..13169
                     /gene="ilvD"
                     /locus_tag="VGC69_12770"
     CDS             11418..13169
                     /gene="ilvD"
                     /locus_tag="VGC69_12770"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0296197.1"
                     /translation="MGNLHKHRSRAIVEGTTRAPHRAFLRSTGMDEAAMQKPFVAIVD
                     TYGENSPCSMSLNQVSDNVRLGVAAGGGVPIRGSAISVTDGTSMNHSGMRMSLVSREI
                     VADSVEVFVRAHGYDALVGVAGCDKSLPGILMGMVRVNLPGVFLFGGAMLPGVAPGEI
                     PGVAGRNRQATILTTIEAVGAAQRGDITREMLDAIEKQCTPGAGSCPGQFTANTMAMV
                     AETLGLAPLGSAMVPSVYSERIAIARRAGETVMRLLKSGGPLPRDLVTHKSLENACAA
                     VAATGGSTNAALHIPAIAHEAGLRFTLDDVQRVFERIPLIGDLQPGGRYLAQDLHHAG
                     GVPAVLNALLAGGYLHGDVPTLDGITLEAALTEFSGPDGTIVRPCNEPLSDGGGLVIL
                     RGNLAPDGACLKIAGLKSLKFSGTARIFECEEDCMAVVAQRAYAQGDVMVIRNEGPKG
                     GPGMREMLSVTAAIYGQGMGEKVALLTDGRFSGATRGLCIGYVGPEAALGGPIALLRD
                     GDRIHIDAQAGTLHVDLSEDELARRRAEAVPFSRGHLGGVLEKYEALVRPAHLGAVTH
                     SGAVAWPYEAPTPGDQA"
     gene            13166..14050
                     /locus_tag="VGC69_12775"
     CDS             13166..14050
                     /locus_tag="VGC69_12775"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0296198.1"
                     /translation="MSPAQVGFCGLGRMGRPMARRLLDAGHTLYAWNRSPDPAHALAA
                     QSPEHCIACATPADVARNADCVMLCLTDGAAVEAVVFGAQGLASAAPAAPTIVDHSSI
                     PPALTRTLAARWHTATGGAWIDAPVSGGTAGAAAGTLAVMAGGAAAAIDAAGPVLASY
                     AARVTRMGGIGAGQTTKLANQTIVVATIAAIAEAVLLAQRTGIDAARMPDALRGGWAD
                     SVLLQTLLPRMLTPPEQASGTIRVMLKDLDAVHALGGEYGAALPLADTVRRWLRRAVD
                     AGYGDADISQLVRVPLDD"
     gene            14132..15448
                     /locus_tag="VGC69_12780"
     CDS             14132..15448
                     /locus_tag="VGC69_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296199.1"
                     /translation="MATTAELSLPSGTQEHKRVLTATLVGTTIEWYDFFVYAQAAGLV
                     FGPAFFAPVERQNPGLAQILSFATIGISFLFRPFGAAFCGHLGDRFGRRAVLAFTLIM
                     MGAATALIGLLPTYAAIGAWAPTLLIVLRVLQGISAGGEWGGAALMSVEYAPANRRSL
                     FGAAPQIGTPLGMIISTGVLWFLTEALGRQAMTEWGWRLPFLFSIVLIVIGAIVRRSV
                     AESPVYEAMRARRAQSSAPLAELLRHHLGKVISAALIFMANNAVGYIVIAFFISYGQK
                     TLHISPNQMLLIGTLSAGGWFVFTLLGGWLGDRLGRVRTFQIGYGLMAVWTIPMWFLI
                     DTGSPALFLFAALVLTAALGLSYGPQAALYAEMFPAKVRYSGVSLGYALGAILGGAFA
                     PAIAQWLITSFGQSWTIGVYLAALSVISLIAVSLVKDTSKVDLRAS"
     gene            15632..>18034
                     /gene="ileS"
                     /locus_tag="VGC69_12785"
     CDS             15632..>18034
                     /gene="ileS"
                     /locus_tag="VGC69_12785"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY0296200.1"
                     /translation="MDYKKTLNLPDTPFPMRGDLAKREPVWVAEWEENRVYAAIRAAS
                     AGRPRFVLHDGPPYANGDIHIGHAVNKILKDIVVKSRNMAGYDAHYVPGWDCHGMPIE
                     IQIEKKYGKNLPVAEMQSKARAYAYEQIDRQRKDFKRLGVLGDWDRPYLTMNFSNEAN
                     EIRVLGRIMEKGYVFRGLKPVNWCFDCGSALAEAEVEYADRTDPAIDVAFPFADKAAL
                     AAAFGLSSIEDGAAVIWTTTPWTIPANQALNVHPEFDYALVRVSPAPAHGPLLLLAAD
                     RVEACLKNWKLQGEVIATAKGAALAGIAMRHPLARLDAGYDRLSPIYLGEYVTLDAGT
                     GIVHSSPAYGIEDFISCKAHGLKDDDILSPVMGDGHYIGTLPLFGGLMIWDANPKVIE
                     ALRSAGSLMDVVQTPHSYMHCWRHKTPIIYRATSQWFAGMDVEPNDDGPTLRESALKA
                     IDETVFYPAWGRARLHNMIANRPDWTLSRQRQWGTPMAFFVHKETGELHPRTLELLEQ
                     VAQRVEQGGIEAWQTLDPRDLLGDEAEQYEKNKDTLDVWFDSGSTHATVLGGKDHDLQ
                     GSHADGLAWPADIYLEGSDQHRCWFHSSLLTGCMLYGRAPYKALLTHGFAVDGQGRKM
                     SKSVGNVVAPQKVSDSLGAEILRLWVASTDYSGELAISDEILKRVVEGYRRIRNTLRF
                     LLANLADFDAATQALPHSELLEIDRYALSMTAQMQAEVTGHYQSYDFHPAVSRLQTFC
                     SEDLGAFYLDILKDRLYTTAPDSRARRSAQTALLDVTQTLLRLMAPILSFTAEEAWKV
                     LLD"
BASE COUNT         2961 a         6155 c         6031 g         2887 t
ORIGIN      
        1 gggcttctat gccgacgcgc ggcgcgctga acggcagaag gccatggccg agcacggcga
       61 actcggcaag cgggccggcg agctggatga gcaatggctg gcgctccagg aagaactgga
      121 agcgctggcc tgagcctggc cagccgcctc gcgggcggcg tgcgcgtcga tggctggccg
      181 gcacgcccgc gtccccgtca ggcgccgggc gcatgggcag tttgttccga aggcgagaac
      241 atcgccacgc agttcctgcc ctgctccttg gcgtcgtaca aggcctggtc cgccatgcgg
      301 atgagcgcct cggggctggc ttcgccttgc gtgccggcga caccgatgct cacggtgaca
      361 acgccggatg gactgcctga atgcgggatc ccgagtcgct ccgttgccgc gcggatccgt
      421 tccgcgaccc gcatcgcctg ctccggcctc gtcccttcga gaatggcgac gaactcttcg
      481 ccgccatacc gcgcgaaatg atcggaaagg cgctgcaatt cggcatttgc cgctgcggcc
      541 acccggcgca gcgcctggtc ccccgcttga tggccatagc tatcgttgta gaccttgaac
      601 ttgtcgaggt cgagaaaaag cagcgacagc gcggtacccg tgcgcgaagc gcgcgcgcac
      661 gcatttcgaa tggcccggtc gaaagcgccc cggttttcaa gttcggtcag cgcatccagg
      721 tcggcttgcc gctggagcgc gatctcggcc ttgcgccgct ggcgcaattc tcgcgcgaac
      781 aagaaactgg ccggcagcac caccaggctg aacagggcgc caagcacgga tatcagcaac
      841 gagcgcttgc gccactcggc aagaatatcg agcgccgaag gcgccacgct gaggatgagg
      901 ggaaatgtct ccagcctctt gaacacgtac aggcgcctga cattatcaag gccggccgtc
      961 ccccagacga cgcccgaggg ttcctgggtc aatttccgga aaacagcccc ggtgttgaag
     1021 ttccgaccga ccagggaggg aacatgggga atccgcgcca acaggctgcc atccgtcatg
     1081 atgatcgccg cggaaccgaa tcggttcagg tcgatcccat cgagcagcga gtggaaatac
     1141 gccacatcga tcgtgccgac gaccacgcca gcgaagctgc cgtcggcgct gctcacgcgc
     1201 ctgctgacgg ttatctcggt ctcgtcggga cggcgcttcg accggtgcgg cgggcttatc
     1261 cacaagccca gcgccggatc ctgccgctgc acctggtagg acttgcggtc ggaaaaatcg
     1321 gcgaccggtg tccgactcgc ggccgccgag tcggcaagca ccttgccatt cgcgtcgagc
     1381 aatacgatag tcccgaaata cggagccgtt gccgaatgat cgaacagcac gcggttcctg
     1441 agccgcgcgg gcaaggacat gatttcagga tcccccgcgc cgtttatgac ggcccgcaag
     1501 gacaagtcat atgaacggat cgtgcgctcc agatcccttt gtatggccag cgacaagttt
     1561 ttcgaacttt ccgtggcaag ccggatcgca tcctgatagg caagataaac cagccagcag
     1621 cacaaggcgg cgacgccgac ccacagtgcc gcaccggtcg cgagaacacc gcgaggcgtg
     1681 agaagcctgg atatcgacag cagcaccagc cgcttgaatc cacagtatat ctgcaccatg
     1741 cggagccccc cgatccgggt tatttgcagc gggcgcaacg gtcgcggcgg cggctaccct
     1801 gcccgttttc agtatcgtcc aaaaaccgcc cctagatgat ctcgagtttg gcgatgccca
     1861 gcgccagcgt cttctggccc acatcccgga actggatctg cgcctgggcg tcctgccccg
     1921 tgccgctcag gcctatgatc gtgccgtcgc cgaagcgcgc atgacgcacg ccctggccga
     1981 tgcggtattg cacctcgccc accatgacgc cgctggacat cgtgcgcgcc gcgcgcggcg
     2041 cgacgttgct ggcatgcgcg cggccaaagg catcgccgcg ccagttgcct gacgcgccgc
     2101 cgcggccgtt gttgaaatcg tcgccccacg aaccgccgcg cgccagcccg gccttgggcg
     2161 tgatccactt gagatgctgc tcgggaatct cgtcgagaaa gcgcgagcgc atggcgtagc
     2221 gggtctggcc atgcagcatg cggctttgcg ccaggctcag gtagagccgt tcgcgcgccc
     2281 gggtgatggc cacgtacatc aggcggcgct cttcttccag gcccgactgc tcaagaatgc
     2341 tgttctcgtg cgggaacagg ccctcttcga ggccggtgat gaacaccgcg tcgaactcca
     2401 ggcccttggc ggcatgcacc gtcatgagct gcacggcgtc ctggccggcc tcggcctggt
     2461 ggtcgccggc ctccagcgcg gcatgcgaca ggaaggccgc cagcggcgtc atgccctcgg
     2521 ccaccacgcc gtcggcgtcg accagcggcg ggaattcgcc cgcctcgtcg gccgcgggcg
     2581 gcggggcgat gccggccggc aagccatcgt agttttcttc gttggcgaac acggccgccg
     2641 cagtgatgag ttcgttgagg ttctcgagcc gctcggcgcc ttcgcgctcg gtcttgtagt
     2701 gggcgatcag gccgctgccg tcgagcatgt gcccgaccaa ttcggtcagc ggcagctcgc
     2761 gggtttcctg cgccaggcgc gtgatgagct gggcgaactg cgccaggttg gagccgccct
     2821 tgcccgccac catggccacc gcgccgtaca ggctggtgcc gtgcgtgcgg gccgcgtcgg
     2881 ccagctgctc gagcgtgcgg gcgccgatgc cgcgcgtggg aaagttcacc acccgcatcc
     2941 acgaggtatc gtcgtgcggg ttggccatca ggcgcaggta ggccagcgcg tgcttgattt
     3001 cctggcgctc gaaaaagcgc agcccgccat agaccttgta gggaataccc gccgagaaca
     3061 ggttgtgctc gatcacgcgc gactgcgcat tgctgcggta gagcacggcg atttcacggc
     3121 gcagccggcc gtcggcgacc agggcgcgca cttcgtcgac cagccattgg gcctccatgg
     3181 cgtcgctggg ctgctcgatc acgcggaccg gctcgccctc gccctggtcg gtccagaggt
     3241 tcttgcccag gcggccgctg ttgtggccga tgagcgcgtt ggccgagtcc aggatgtggc
     3301 cataggagcg gtagttctgc tccaggcgaa tgaccgtgcc gcgggcgtag tcgcgctcga
     3361 aatcggccat attgcccacg tgcgcgccgc gaaaggcgta gatcgactgg tcgtcgtcgc
     3421 ccacggcgaa gacatgcgcg ccgccgcccg ccagcagctg cagccacttg tactgcagga
     3481 tgttggtgtc ctggaactcg tcgaccagga tgtggcgaaa gcgccgctgg tagtgctcgc
     3541 gcaccggcgc gttgcggctc aacagctcgt aggcgcgcag cagcagctcg gcgaaatcca
     3601 ccacgccctc gcgctggcac tggtcttcgt agagctggta gagctcgacc aggcggcggc
     3661 tgtgggcatc ccaggcttgc atctggccgg ggcgctcgcc gccttccttg gcattgttga
     3721 tgaagcgctg cacgtcgcgc ggcgggtatt tctcgtcgtc gacgttgtgg gccttgagca
     3781 ggcgcttgat ggcggccagc tggtcggcgg tgtcgaggat ctggaaagac tgcggcaggc
     3841 ccgcgtcgcg gtaatgcgcg cgcagcatgc ggttgcacag gccatggaag gtgcctatcc
     3901 acaagccgcg cgtgtcgatg ggcaggatgg ccgtcatgcg cgagagcatc tcgcgggcgg
     3961 ccttgttggt gaaggtgacg gccagcaggc tgaagggact ggcctggccc gtctggatga
     4021 gccaggccat gcgggtggtc agcacccgcg tcttgccgct gcccgccccg gcgagcacca
     4081 aggcgtgctg cggttcgagc gtgacggctg cccgttgttc gggattgagt ttgtccagca
     4141 attgcatccc caatacagca aggcccccgc gccacgcctt cggcttgcgg cccctccggg
     4201 gggaccgttt taccctgggg cggcacggcg gcaaaaagca tcggccccca accgggggcc
     4261 gataccgtaa accagtttag cggtaattat gccgcgtagc ggctcaggcg ctttgcaaac
     4321 tgctcgagcc cggcgatgcc gctttgctgg gcgcggtggc accaggcctg cagctcgacc
     4381 agcaactgct cgctgctgga gctggagctt tcccagaggc ggcccagttc gcggcgcatt
     4441 tgcaccaggg tggacagcat ggggtgctgg gcgatggtgc tgtcgacctc ggccaactgg
     4501 tcgggctgca gcatctcttc gttgcgggtg agcgagccgc gcacgcggta cagcttggcc
     4561 cagcgatcgg actggccctg gagcttggag agctcttcgc gggcagcctg ccggatgaca
     4621 tcggtgtagc gggccatgac ttcgtagcgg tgcgtgatca cgccctgcaa ggtgcgcagg
     4681 tcgatggcgc gggccgtggg gtcgagcttg agcttgggcg cgaccttctt gatcttggcc
     4741 aggcccagca tgcgcagcac gctgatgtag ccccagccca cgtcgatctc gaaccacttg
     4801 gccgagaact tggccgaggt gccatgcgcg tggtggttgt tgtgcagttc ttcgccgccg
     4861 atgatgaagc cccaggggat cacattggtg ctggtgtcgg ggctgttgta gttgcgatag
     4921 ccccagtagt ggccgatgcc gttgaccacg ccggccgccc agaagggaat ccaggccatc
     4981 tgcacggccc acacggtcag gccgatggcg ccgaacaggg cgatgtcgat ggccagcagg
     5041 atcagcacgc cagtgaggct gtgcttggcg taaaggtttt gctcgagccg gtcattgggc
     5101 gtgccgtggc cgaacttggc cagggtttcc tggttggccg cctcgacgcg gtacaggtcg
     5161 gcgccgcgga acagcacctt ccagatgccg aacaggatgg gcgagtgcgg atcgccctct
     5221 ttttcgcatt tggcgtggtg cttgcggtgg atggcgaccc actccttggt gaccatgccg
     5281 gtggtcatcc acagccagaa gcggaagaaa tgcatgacgg ccgggtgcag atcgagcccc
     5341 cggtgagcct ggctgcggtg caggaagaca gtgacggaca cgatggtgat gtgcgtcatc
     5401 accagtgtgt agacgacgac ctgccaccaa tgggcttgcg tcagaccgcc ggccaggaaa
     5461 gaaaggatga gatccataaa gctgtggctt acctcaaagg aggtgaatgt gaagaaccag
     5521 tggggattct agcctaccag cctcccgatg tgacagctgg atttcaaata cttcttatgg
     5581 aaaatcaatg actaaggcca aatgccagct tcccgcccgg aaaaaccggc ttcgccgggg
     5641 gccgcacaga aattcgttac aagacctgtc tgaaacggct gtgcatgcta tacagcacgg
     5701 gcccgcccca tcctcgtttt tccgacatgt ccatgctgaa catcgcgcgc cgcctgctgg
     5761 cggcagccgc cctcgccgcc gccgcgccgg ccctggccga cgacatcccg ccgctcgcgc
     5821 ccggccagca aatggtgctg gccaactatt acgaactcag cgccaccggc tgccgcgccc
     5881 tgaatccacc ccgcgtggtc atcacccagc agcccgccct gggcaagctc atcgtggtgc
     5941 gcacccgggg gacggccaac accagttcgc ccaggtgcgc ggtgagcacc atgtccctgc
     6001 cgatctcgca agtgttgtac caggccgaca aacccggcct cgacaccatg gcctgggcca
     6061 tcaagttcca ggcgcgcgtg ggcccgcgcg gcaaggatcc gcgcatcgaa tacggcaacg
     6121 cccggctgca gatcaagcct ggcccggctc gctgatcggc tcgccgcctt ccgcttccgg
     6181 ttcgccggcg acgtcctcct ccggctcggc ctgggccttg gcgcttgaaa cagccttctt
     6241 gcgctcgaac acggcggcaa aaaagccatc cgtgccgtgc acgtccggac gcagctgcat
     6301 gtatgggccg tccaacacca gggccggcac gcgctgggcc agcagggccg cggcatccag
     6361 gcgctcgaag tccggatgct cggccaggaa acgctcggcc tgctgggtgt tttcttcggc
     6421 cagcaggctg caagtggcgt agaccagccg cccgccgggc gccacgcagc gcgcggcgct
     6481 cttcaagatg cggtgctgca gcgcggtcag ctcggccagc gattccggac tctggcgcca
     6541 tttcaagtcg ggattgcggc gcagcgtgcc gatgccgctg cacggcgcat cgaccagcac
     6601 gcgctgggcc ttgccggcca ggcgcttgac gcgcgcatcg ttctcgccct cgatgaccac
     6661 cggcaccacg ttcgacaggc cgctgcgcgc aaagcgcggc ttggcgcgcg ccagccgccc
     6721 ggccgacacg tcgaaggcat agagccggcc ggtggaacgc atcaacgccc ccagcagcaa
     6781 ggtcttgccg cccgcgccgg cgcagaaatc gatgatcatc tcgccgcgct tggggccgac
     6841 cagcagggcc agcaattggc tgccctcgtc ctgcacttcg aggccgccgt gctcgaactg
     6901 tgcccagcgg ttcagggatg ggtgccccgt cacgcggatg ccccagggcg aatacggcat
     6961 gggcaccggg tcgaaacggg ccgaatcccc cccgttgcgc aggacttaca gcaaggcatc
     7021 gcgctcgatc ttgagcggat tggcgcgcag gtccagcggc gcgggttggt tgagcgcctg
     7081 catcaggcgc tcgggctcgg tgtggcggtc caggcgctgc accagccagt cgggcatgct
     7141 gtagcgcact tcgcgcgcca gggtggcgga gtctatcgtc gacacatgcg acagccacgc
     7201 ggcttcgccg ggatccagga catcggccag ccgtgccgcg cccagcgtgg cggtcaggcc
     7261 cagaatggcc aggcggcgca cggccggtcc cacgcccgac tcggccaggc tgcgatagcg
     7321 gcgcaggtgg cgcagcacgt catagaccgc ctcggcgatc tcgctgcgat cgcggctgcc
     7381 cagcctggga ttggcgcgca gccaaaggga aagcaccacg tcggcgggtt gatcccattg
     7441 caggacttcg cccagcacgc gctgcacctg ctcgatgcgc atggccgcca gcggcgggcg
     7501 gggacggtcg tcgcgcggca cgtaggaggc cccctccgcg gcaggccggg agggccggtc
     7561 ggcgcgctcg ggacggccgg gccgggagtc cccggcaggc gccctgtcgg ggcgggagcg
     7621 gggcgcttgg gatttggtca tgaaggttcc agcatgaaaa gacgttgcgg atccccggcc
     7681 acgtcgaccc gttggtcggc ggccagcgtg acccggccct gggccagcca gcgcacggcg
     7741 gcggggaaca cttgatgctc gacctgcagc acccgttcgg ccagcagctc gggcgtgtcg
     7801 ccggccagca cgggcacgca gccctgggcg atgatggggc cgtggtcgag caccggcgtg
     7861 acgaagtgca ccgtgcagcc gtgcacgcgc acgcccgtgg ccagcgcctg ggcatgcgta
     7921 tgcaggccgg gaaaggccgg cagcagcgag ggatggatat tgatcaggcg gcccgcgtag
     7981 cgcgccacga aaccggcggt gagcacccgc atgaagccgg ccagcaccac gtagtcgggc
     8041 cggtggcggt cgatttcctc ggccagggcc gcgtcgaagg cctcgcggct gggatagtcc
     8101 ttgtggctga tcccgcgggc ggcgatgccc tgcccgcgcg cccagtccag gcccggggcg
     8161 tcgggccggc tggcgatcac ggcggcgatc cgggcaggcc aggaatcgcg ctggcaggcc
     8221 tcgaccagcg cctgcatatt gctgccgcgg cccgagatga ggatgacgaa acggcgcggc
     8281 cgcggcgcgg cggcggggga ggactggaac gcggcggacg gggatgcggc ggaatccggc
     8341 aagatgggtt ccaaatttca ggaaatcagg gaaagactta aattgtaaac tctcgcctgt
     8401 gaaacccatg ccgcgcatct tccgccatac cgcctccgcc gccccgcgcc ccgcgtgcgc
     8461 cctgaccatc ggcaacttcg atggcgtgca tctcggccac caggccatcc tggccagcgt
     8521 gcgccgggag tcccaggtcc gcggactggt gccggcggtc atgaccttcg aaccgcatcc
     8581 gcgcgaatac ttcgccgcgc tctaccagcg ccccgagctc gcgcccacgc gcatttcggg
     8641 cctgcgcgac aagctctccg ccctggtcca ggccggcatc gaggaaatct tcgtgcagcg
     8701 cttcgacgcg cgcctggccg ccatgtcggc caacagcttc atcgaagacc tgctggtgcg
     8761 cgacatgaac gtgcgctggc tgctggtggg cgaggacttc cgcttcggcc acaagcgcag
     8821 cggcgacatc gagctgctgc gcgaagccgg cctggcgcac ggcttcgagg tccgcaccct
     8881 ggccgacgtc gccgacgcgc acggccaccg catttccagc tccgaggtgc gcaccgcgct
     8941 ggcagtgggc gacctgccgc gcgcccagca cctgctgggc catccctatc gcctgagcgg
     9001 ccacatcatc cacggccaga agctgggccg gcagctgggc tttcccacca tgaacctgcg
     9061 cgtggcgccg cgctgcgcgg cgcgctcggg catctacgtg gtgcgcgtgc tgggcctggg
     9121 cgacgcgccg ctgcccggcg tggccagcct gggcgtgcgc ccgaccgtgg aagacggcgg
     9181 gcgggtgctg ctcgaagcgc acgtgctcga tgcccgcgtc gacgcttacg ggaaactggt
     9241 gcgcatcgag ttcctgcgcc atctgcggga tgaggaaaaa ttctccgacc tccccgcgct
     9301 aaccgccgcc attgccgaag acgcacgcaa tgcgcgcgcc tattttgccg ctcacggact
     9361 ataaaagttc cgcaatccca ggcgcatgcg ccggcgcggg cggcgccccg ggattattcg
     9421 ttgaaatgct ccgccgaagt ctctcgcagg cattcatgga atagcgacgt ggcgggctcg
     9481 gtcgagtcgg tgcgccagta tgcgacgacc tcgcccagcg cttccagcgg cggcagcggc
     9541 aacagccgca tggtgccgcg cgtcatatga ccgcgcgcca ccgatagcgg caggatcgac
     9601 acgaaatccc cgctttcgat cagcgtgacg ttcagcgcaa gggagctgga ttcgaccgcg
     9661 ctgcccgcga gcgcgacgtc atgcgcgccc aatgtttcgg cgagcgacat gaatgccggc
     9721 gagctgcgca agggcacgac ccaccgataa ggccgcagat cctcccaact gaccggacgt
     9781 tcgggatttc ccagcgcatg gccaacgccg gccacgaaga caaacggctc ccggtacagg
     9841 cattcctgcc gcagtcgcat ttccacgccg gtcaggtggt tgcggcccaa ggcgatatcg
     9901 agttcgccgt catcgagcag cttgatcagg cggtcgagcg tcgcttcgac gaacgagaac
     9961 gacgacgagg gcgcgcgtcg catgaacaac cgcaccgcgc tggcgatgat cggctcggga
    10021 atggtcacga cggcgcccac gcggacgtgc ccgccggtgc cggccgcaag cgcgctgacg
    10081 tcgcgccggg caagctcgat atgccgcagg atctcgcgcc catgctcgac caggaccctg
    10141 ccgatgccgg tcagcccgac ggcatttccc agccgctgca cgatttgcac atcgagcgcg
    10201 ctctcgattt ccgcgatctg cttggaaatg gcgggctgcg tcacgtgaaa ggcggccgcc
    10261 acgcgcgtca cctgccccag ttcggccagg ctcacgagca tgcgcaaatg ggacagcttc
    10321 aggccgctgc ggaaaaactg ctcgatcgga tcgctgttgc gcgctggcat cacgttcttg
    10381 agaaaataac ttggcagtta tgacttgtac caaaaaagtg atttgccgtg tcgggccgcg
    10441 cctcgataag ctccgagaac agcgatccat cgtgataggc gggcacggcc aggtcaatat
    10501 aactggcacg atatgtcccg ccacgaaaaa cgcaaagaac aaggctgccg gataccccct
    10561 gtgcagccca taagtccgga gacagacatg acgcattcca agcaacccgt ctgcatgatc
    10621 acgggtgcgg cgaccggtat cggcgcggct tgcgcgcgac gctttgcgcg cgccggctgg
    10681 gcggtcgcca tcggcaattt cgacgacggc acgcgcgccg cggcactggc gaccgaggcg
    10741 gcgtgccacg gcgacggggc gcccgcgctc gtgttcgacg cggacgtcgg cgcgaacgac
    10801 gattgccgcc gcgccgtcga cacagtcgca acggcctggg ggcgcatcga cgccctgatc
    10861 aattgcgcgg gaaccacgcg cttcgtaccg cacgcggacc tcgcggcgct cgacgatgcg
    10921 gagttcgagc gcgtctatcg cgtgaacctc atcggcatgt tccagatgac gcgcgctgcc
    10981 gaatccttgc tgcgcgagac ggcgacggcc gggcgaagcg ccagcgtcat caatatttcg
    11041 tccctggcgg ggctcaacgg cacgggctcc tcgatcgcct atgccgcgtc gaagggcgcg
    11101 gccaacaccc tgacgctgtc gctggcgcgc agcctcgcgc ctcatatccg cgtgaacgcc
    11161 atcgcaccgg gcatggtcga tgatggcttg ctcaaccgcg tgctcgacgc cgccgcatac
    11221 gagcgcgtcg tcgcccgcat gatcgaggcc gccccgctca agcgcgtgtc gcagcccgcc
    11281 gaagtggcgg aattggcctg gttcatcgcc gcgcacgcgc ccgcgctcac cggacaggtg
    11341 atcgccatgg aaaacggctt gatgcttgcc ggcggctaga tccgccgctt gctgctttca
    11401 aaaaaagggc ttcattgatg ggtaacctac acaagcaccg ctcgcgcgcc atcgtcgaag
    11461 gcacgacgcg cgcaccgcac cgcgcgtttc tgcgctccac cggcatggac gaggcggcca
    11521 tgcaaaaacc gttcgtcgcg atcgtcgata catacggcga gaattccccc tgttcgatgt
    11581 ccttgaacca ggtttccgac aacgtgcggc tgggcgtggc ggccggcggc ggcgtgccga
    11641 tccgcggctc ggcgatctcg gtgaccgacg ggacgtcgat gaaccactcc ggcatgcgca
    11701 tgagcctggt gtcacgcgag atcgtcgccg acagcgtcga ggtattcgtg cgcgcccacg
    11761 gctatgacgc gctggtcggc gtggccggct gcgacaaatc gctgcccggc atcctcatgg
    11821 ggatggtgcg cgtcaacctg cccggggtgt tcctgttcgg cggcgccatg ctgccgggcg
    11881 tggcccccgg ggagattccc ggcgtcgcgg ggcgcaaccg gcaggcgacg atcctcacca
    11941 cgatcgaagc ggtgggcgcg gcgcagcgcg gcgacatcac gcgcgaaatg ctcgatgcca
    12001 tcgaaaagca atgcacgccg ggcgcgggct cctgcccggg ccaattcacc gcgaacacga
    12061 tggccatggt cgccgagacg ctcggcctgg cgccgctggg ctcggcgatg gtgccctcgg
    12121 tgtatagcga acgcatcgcg atcgcgcggc gcgcgggcga aaccgtcatg cgcctgctga
    12181 agtccggagg gccgctgccg cgcgacctcg tcacgcacaa gagcctcgag aacgcgtgcg
    12241 cggcggtcgc ggccaccggc ggttcgacca acgccgcctt gcatattccc gcgattgccc
    12301 acgaggcagg cctgcgcttc acgctcgacg acgtgcagcg cgtcttcgag cggattccgc
    12361 tgatcggcga cctgcaacca ggcggccgct atctcgccca ggacctgcac catgccggcg
    12421 gcgtgcccgc cgtgctcaat gccttgctgg cgggggggta tctgcatggc gacgtcccga
    12481 cgctcgacgg catcacgctc gaggcggcat tgactgaatt ttccggaccg gacggcacca
    12541 tcgtgcgtcc ctgcaatgaa ccgctgagcg acggcggcgg cctggtcatt ctgcgcggca
    12601 acctggcgcc cgacggcgcc tgcctgaaga tcgccgggct caagtccctg aaattctccg
    12661 ggacggcgcg catcttcgaa tgcgaggaag actgcatggc ggtcgtcgcg cagcgcgcct
    12721 atgcgcaggg cgacgtgatg gtgatccgca acgaagggcc caagggcggc cccggcatgc
    12781 gcgagatgct gagcgtgacg gccgcgatct acggacaggg catgggcgag aaggtcgccc
    12841 tgctgaccga cggacggttt tccggcgcga cgcgcggcct gtgcatcggc tatgtcgggc
    12901 cggaagcggc gctgggcggc ccgatcgcgc tgctgcgcga cggcgaccgc atccatatcg
    12961 acgcgcaggc gggcacgctg catgtcgacc tgtccgagga cgagctggcg cgacggcgcg
    13021 ccgaggcggt gccgttttcg cggggacacc tgggcggcgt gctcgagaaa tacgaagccc
    13081 tcgtccggcc cgcgcacctc ggcgcggtca cgcattcggg cgccgtcgcc tggccctatg
    13141 aggcgccgac gcccggagat caggcatgag tccggcgcag gtgggtttct gcgggctggg
    13201 ccggatgggc aggccgatgg cgcgccgcct gctcgatgcc ggccatacgc tgtacgcctg
    13261 gaatcgttcg cccgaccccg cgcacgcgct ggcggcccag agcccggagc actgcatcgc
    13321 ctgcgcgacg ccggccgacg tcgcgcgcaa cgcggactgc gtcatgctgt gcctgaccga
    13381 cggcgccgcc gtcgaagcgg tcgtcttcgg cgcgcaaggg ctcgcgagcg cggcacccgc
    13441 cgcgccgacc atcgtcgacc attcaagcat cccgcccgcg ctgacgcgga ccttggcggc
    13501 gcgctggcat acggcgaccg gcggcgcatg gatcgacgcg ccggtgtcgg gcggcaccgc
    13561 gggcgcggcg gccggaacgc tggcggtcat ggcgggcggc gctgccgccg ccatcgacgc
    13621 cgccggcccg gtgctcgcca gctacgcggc gcgcgtcacc cgcatgggcg gcatcggcgc
    13681 cgggcagacg accaagctcg ccaaccagac catcgtcgtc gccaccatcg cggcgatcgc
    13741 cgaagcggta ttgcttgcgc agcgaacggg tatcgacgcg gcgcggatgc cggacgccct
    13801 gcgcggcggc tgggccgatt cggtgctgct gcagacgctg ctgccgcgca tgctcacgcc
    13861 gcccgagcag gcgtcgggca cgatccgcgt catgctcaag gacctcgacg cggtccatgc
    13921 gctcggcggc gaatacggcg cggcgctgcc gcttgccgat acggtgcggc gctggctcag
    13981 gcgcgcggtc gatgccggat acggcgacgc cgatatttcg caactcgtgc gagtgccgct
    14041 ggacgactga cgcttcatca caaccggccg cgatcgaatc gcatggcgat acgcgccgga
    14101 ccttcaaaaa accatgcccg aggagacaac aatggcgacc acagccgaac tttccctgcc
    14161 gagcggcacg caggaacaca aacgcgtgct gaccgcgacc ctggtcggaa cgacgatcga
    14221 atggtatgac ttcttcgtct acgcgcaggc cgccggcctg gtgttcgggc cggcgttctt
    14281 cgccccggtc gaaaggcaga accccggcct ggcgcagatc ctgtcgttcg ccacgatcgg
    14341 catttcgttt cttttccgtc cgttcggggc agccttctgc gggcacctcg gcgaccggtt
    14401 cggccggcgc gccgtcctgg ccttcacgct gatcatgatg ggggcggcga ccgccctcat
    14461 cggcctgctg ccgacctacg cggcaatcgg cgcctgggcg ccgacgctcc tgatcgtgtt
    14521 gcgcgtgctg cagggcatct cggccggcgg cgagtggggc ggcgccgcgc tgatgtcggt
    14581 cgagtacgcg cccgcgaacc ggcgcagcct gttcggcgcc gcgccgcaga tcggcacgcc
    14641 gctggggatg atcatctcga ccggcgtgct gtggttcctg accgaagcac ttggccggca
    14701 ggcgatgacc gaatggggct ggcggctgcc gttcctgttc tccatcgtcc tgatcgtcat
    14761 cggcgccatc gtgcgccgct ccgtcgcaga atcgccggtc tacgaagcga tgcgggcgcg
    14821 ccgagcgcaa tcgtccgcgc cgctggccga actgctgcgc catcacctcg gcaaggtcat
    14881 cagcgcggcg ctgatcttca tggccaacaa tgccgtcggc tacatcgtga tcgccttttt
    14941 catcagctac ggccagaaga cgctccacat atcgcccaac cagatgctgc tgatcggcac
    15001 cttgtcggcg ggcggatggt ttgtcttcac gctgctcggc ggctggctgg gcgaccgcct
    15061 gggccgggtg cggacattcc agatcggcta cgggctcatg gcggtctgga ccatcccgat
    15121 gtggtttctc atcgatacgg gcagtccggc gctgttcctg ttcgccgcgc tggtgctgac
    15181 cgcggcgctc ggcctttcgt acgggccgca ggcggcgctg tatgcggaaa tgtttccggc
    15241 caaggtgcgc tattccgggg tgtcgctagg gtatgccctg ggcgccatcc tcggcggcgc
    15301 gttcgcgccc gcgatcgcgc aatggctgat cacgagcttc gggcagtcgt ggacgatcgg
    15361 cgtctacctg gcggcgttgt cggtgatttc cttgatcgcg gtctcgctgg tcaaggacac
    15421 gagcaaggtg gatctccggg cttcctagcc gcctggcagg tcgttcacgg agcatccccg
    15481 cggggtggcg caatgaccgg attgcgccac cccgttcaat ttggatgccc aggttttggt
    15541 gacggatgtc ccgccggaaa taaaggccgt ccccgtctgc taatctatat ggttgcactg
    15601 atcaaaattt gcccggatac gcctagttct catggactat aagaaaaccc tcaatctgcc
    15661 cgataccccc ttccccatgc gcggcgatct cgctaagcgt gaacccgtct gggtcgcgga
    15721 gtgggaagaa aatcgcgttt acgcggccat ccgcgccgcc agcgccggcc gcccgcgctt
    15781 cgtgctgcac gacggcccgc cctacgccaa cggcgacatc cacatcggcc acgcggtcaa
    15841 caagatcctg aaggacatcg tcgtcaagag ccgcaacatg gccggctacg acgcgcacta
    15901 cgtgcccggc tgggactgcc acggcatgcc catcgagatc cagatcgaga agaagtacgg
    15961 caagaacctg ccggtggccg agatgcagtc caaggcgcgc gcctatgcct acgagcagat
    16021 cgaccgccag cgcaaggact tcaagcgcct gggcgtgctg ggcgactggg accggcccta
    16081 cctcaccatg aacttcagca atgaggccaa cgagatccgc gtgctcgggc gcatcatgga
    16141 gaaaggctat gtattccgcg gcctgaagcc cgtgaactgg tgcttcgact gcggctcggc
    16201 cctggccgag gccgaggtcg agtacgccga ccgcaccgac ccggccatcg acgtggcctt
    16261 tcccttcgcc gacaaggcgg ccctggccgc cgccttcggc ctgtcgtcca tcgaggacgg
    16321 cgccgccgtc atctggacca ccacgccctg gaccattccc gccaaccagg cgctgaacgt
    16381 gcaccccgag ttcgactacg cgctggtacg cgtctcgccc gcgccggccc atgggccgct
    16441 gctgctgctg gccgccgacc gcgtcgaggc ctgcctgaag aactggaagc tgcagggcga
    16501 agtgatcgcc acggccaagg gcgccgcgct ggccggcatc gccatgcgcc acccgctggc
    16561 caggctggac gcgggctacg accgcctctc gcccatctac ctgggcgaat acgtcacgct
    16621 ggacgccggc accggcatcg tgcactcgtc gcccgcctac ggcatcgagg acttcatctc
    16681 ctgcaaggcg cacggcctga aggacgacga catcctcagc cccgtgatgg gcgacggcca
    16741 ctacatcggc accctgccgc tgttcggcgg cctgatgatc tgggacgcca accccaaggt
    16801 catcgaggcc ctgcgcagcg ccggctcgct gatggacgtg gtgcagaccc cgcacagcta
    16861 catgcactgc tggcgccaca agacgcccat catctaccgc gccaccagcc agtggttcgc
    16921 cggcatggac gtcgaaccca atgacgacgg ccccacgctg cgcgaaagcg cgctcaaggc
    16981 catcgacgaa acggtcttct accccgcctg gggccgtgcg cgcctgcaca acatgatcgc
    17041 caaccgcccc gactggacgc tctcgcgcca gcgccagtgg ggcacgccca tggccttctt
    17101 cgtgcacaag gaaaccggcg aattgcaccc gcgcacgctc gagctgctcg aacaagtggc
    17161 gcagcgcgtc gagcaaggcg gcatcgaggc ctggcagacg ctcgacccgc gcgacctgct
    17221 gggcgacgag gccgagcagt acgagaagaa caaggacacg ctcgacgtgt ggttcgactc
    17281 cggctccacc cacgccaccg tgctgggcgg caaggaccac gacctgcagg gttcgcacgc
    17341 cgacggcctg gcctggccgg ccgacatcta cctggaaggc tccgaccagc accgctgctg
    17401 gttccactcg tcgctgctga ccggctgcat gctgtatggc cgcgcgccct acaaggcgct
    17461 cctgacccac ggcttcgccg tcgacggcca gggccgcaag atgagcaagt cggtgggcaa
    17521 cgtggtcgcc ccgcagaagg tgtccgattc gctgggcgcc gagatcctgc gcctgtgggt
    17581 ggcctcgacc gactattcgg gcgaactggc catttccgac gagatcctca agcgcgtggt
    17641 ggaaggctac cgccgcatcc gcaacacgct gcgcttcctg ctggccaacc tggccgactt
    17701 cgatgccgcc acgcaagccc tgccgcacag cgagctgttg gaaattgacc gctatgccct
    17761 gagcatgacc gcgcagatgc aggccgaggt gaccgggcac taccagtcct acgacttcca
    17821 tccggcggtg tcgcgcctgc agaccttctg ctcggaagac ctgggcgcct tctacctgga
    17881 catcctcaag gaccgcctct acaccacggc ccccgacagc cgcgcgcgcc gctcggcgca
    17941 gaccgccctg ctggacgtca cgcagacgct gctcaggctg atggcgccca tcctgtcgtt
    18001 cacggccgag gaagcctgga aggtcctgct ggat
//
LOCUS       DASWPO010000245         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2917524, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000245 DASWPO010000000
VERSION     DASWPO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2917524"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3053
                     /locus_tag="VGC69_12790"
     CDS             <1..3053
                     /locus_tag="VGC69_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY0296201.1"
                     /translation="LVSFIGPKPNLLDINNVNPPLRLEVAQPVLDFADMAQIRSIEQI
                     TSKKFRSYELDITYPAAWGPEGIEARVAALCARAVDAIKSGYNILIVSDRLVDNERVA
                     IPALLAVSAVHQHLIRAGLRTATGLVVETGSAREVHHFALLGGYGAEAIHPYLALESL
                     GKLDEPARAVKNYIKAVGKGLNKVMSKMGISTYMSYCGAHIFEAVGLQRELIEKYFTG
                     TASNIEGIGIFQVAEEALRLHRAAFGNDPVLAQDLDAGGEYAFRIRGEEHMWTPDSIA
                     KLQHSARANNYRTYKEYAQIINDQSKRHMTLRGLFEFKVDPSRAIALDEVEPAKEIVK
                     RFASGAMSLGSISTEAHSTLAVAMNRLGGKSNTGEGGEDELRYRDELRTGKTNIKDGD
                     TLASVLGSDRIAADVALRKGDSMRSRIKQVASGRFGVSAEYLSSADQIQIKMAQGAKP
                     GEGGQLPGHKVTDYIAKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNSRASI
                     SVKLVSEVGVGTVAAGVAKAKADHVVIAGHDGGTGASPVSSIKYAGTPWELGLAETQQ
                     TLVLNRLRSRIRVQADGQMKTGRDVAIGALLGAEEFGFATAPLVVEGCIMMRKCHLNT
                     CPVGVATQDPELRKKFSGKPEHVVNFFFFVAEEVREIMAQLGIRKFGDLIGRTDLLDM
                     RAGIDHWKAHGLDFSRVFHRPVPVGDDAIFQVEEQDHGLAGALDHQLIERSKPALERG
                     EKVSFITPVRNRNRTIGAMLSGAVAARYGHEGLPDDTVHIQCNGTAGQSFGAFLAHGI
                     TLDLVGEGNDYVGKGLSGGRIIVRSPNDFRGFGPEHIIAGNTVLYGALAGEAYFNGVA
                     GERFAVRNSGAATVVEGTGDHGCEYMTGGTVVVLGHTGRNFAAGMSGGVAYVWDPDRS
                     FRQRCNLTMVELEPVLPHAEQSGTNAQATWHSAQRGGERQPDETLLRRLVEEHFRHTG
                     SFRAREILGDWEASRGKFVKVMPTEYKRALAELWQAANPEQMVA"
     gene            3237..>4694
                     /locus_tag="VGC69_12795"
     CDS             3237..>4694
                     /locus_tag="VGC69_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY0296202.1"
                     /translation="MGKITGFMEYQRLSEAYEAPQKRLKHWSEFVVHLTDDQAKQQAA
                     RCMDCGIPFCNNGCPVNNIIPDWNDLVYRQQWQQALHVLHSTNNFPEFTGRICPAPCE
                     AACTLNINSDAVGIKSIEHAIIDKGWAEGWVQPLLPARKTGKKVAVVGSGPAGLACAQ
                     QLARAGHAVTVFDKSDRIGGLLRYGIPDFKLEKTLIDRRIAQMEAEGVEFAPSTYIGK
                     ASEAAADGLTVRTPESLMADFDAVVMAGGSETPRDLPIPGRELSGIYYAMDFLRPQNK
                     AVAGDRLADQPSAAGKHVVVIGGGDTGSDCVGTSNRHGAASITQFEINEQPPETENKP
                     LVWPYWPLKLRTSSSHDEGCQRDWSVVTKSFSGSNGKLEKLVAVHVAWARDEATGQMK
                     MAEVPGSEFEIKADLVLLAMGFVSPVQTVLDAFGVDRDTRGNIRANTDDYRTNVDKVF
                     AAGDMRRGQSLVVWAIREGRQCARSVDEFLMGSTEL"
BASE COUNT          863 a         1707 c         1478 g          646 t
ORIGIN      
        1 cgctggtgtc gttcatcggc cccaagccca acctgctcga catcaacaac gtcaacccgc
       61 cgctgcgcct cgaagtcgcg cagcccgtgc tggacttcgc cgacatggcg cagatccgca
      121 gcatcgagca gatcaccagc aagaagttcc gcagctacga actggacatc acctacccgg
      181 ccgcctgggg ccccgaaggc atcgaggccc gcgtggccgc gctgtgtgcg cgcgccgtcg
      241 acgcgatcaa gagcggctac aacatcctga tcgtgtccga ccgcctggtc gacaacgaac
      301 gcgtggccat cccggcgctg ctggccgtgt cggccgtgca ccagcacctg atccgcgccg
      361 gcctgcgcac cgccaccggc ctggtggtgg aaaccggctc ggcgcgcgaa gtgcaccact
      421 tcgcgctctt gggcggctac ggcgccgaag ccatccaccc ctacctggcg ctcgaatcgc
      481 tgggcaagct ggacgaaccc gcccgcgccg taaagaacta catcaaggcc gtgggcaagg
      541 gcctgaacaa ggtcatgtcc aagatgggca tctcgaccta tatgtcgtac tgcggcgcgc
      601 acatcttcga ggccgtgggc ctgcagcgcg agctgatcga gaagtacttc accggcacgg
      661 cgtccaatat cgaaggcatc ggcatcttcc aggtggccga ggaagccctg cgcctgcacc
      721 gcgccgcctt cggcaacgac cccgtgctgg cgcaggacct ggacgccggc ggcgaatacg
      781 ccttccgcat ccgcggcgaa gagcacatgt ggacgcccga ctcgatcgcc aagctgcagc
      841 attcggcgcg cgccaacaac taccgcacct acaaggaata cgcgcagatc atcaacgacc
      901 agtccaagcg ccacatgacg ctgcgcgggc tgttcgagtt caaggtcgat cccagccgcg
      961 ccatcgccct ggacgaagtc gagcccgcca aggaaatcgt caagcgcttc gcctccggcg
     1021 ccatgtcgct gggctcgatc tccaccgagg cgcactccac gctggccgtg gccatgaacc
     1081 gcctcggcgg caagtccaac accggcgagg gcggcgagga cgaactgcgc taccgcgacg
     1141 aactgcgcac cggcaagacg aacatcaagg acggcgacac gctggcctcg gtgctgggct
     1201 ccgaccgcat cgccgccgac gtggcgctca ggaagggcga ctcgatgcgc tcgcggatca
     1261 agcaggtggc ctcgggccgc ttcggcgtca gcgccgagta cctgtcctcg gccgaccaga
     1321 tccagatcaa gatggcccag ggcgccaagc ccggcgaggg cggccagctg cccggccaca
     1381 aggtcaccga ctacatcgcc aagctgcgct actcggtgcc gggcgtgggc ctgatttcgc
     1441 ccccgccgca ccacgacatc tactccatcg aagacctggc ccagctcatc cacgacctga
     1501 agaacgtcaa cagccgcgcc tccatctcgg tcaagctggt gtcggaagtg ggcgtgggca
     1561 cggtggcggc cggcgtggcc aaggccaagg ccgaccacgt cgtgatcgcc ggccatgacg
     1621 gcggcaccgg cgcctcgccc gtgtcgtcga tcaagtacgc cggcacgccc tgggaactgg
     1681 gcctggccga gactcagcag acgctggtgc tcaaccgcct gcgcagccgc atccgcgtgc
     1741 aggccgacgg ccagatgaag acaggacgcg acgtcgccat cggcgcgctc ctgggcgccg
     1801 aggaattcgg cttcgccacc gcgccgctgg tggtcgaggg ctgcatcatg atgcgcaagt
     1861 gccacctgaa cacctgcccg gtgggcgtgg ccacgcaaga cccggaactg cgtaagaagt
     1921 tctcgggcaa gcccgagcac gtggtgaact tcttcttctt cgtcgccgag gaagtgcgcg
     1981 agatcatggc ccagctgggc atccgcaagt tcggcgacct gatcggccgc accgacctgc
     2041 tcgacatgcg cgccggcatc gaccactgga aagcccacgg cctggacttc tcgcgcgtgt
     2101 tccaccgccc cgtgcccgtc ggcgacgacg ccatcttcca ggtcgaggaa caagaccacg
     2161 gcctggccgg cgcgctcgac caccagctca tcgagcgcag caagcccgcg ctggagcgcg
     2221 gcgagaaagt ctccttcatc acccccgtgc gcaaccgcaa ccgcaccatc ggcgccatgc
     2281 tgtcgggcgc ggtggccgcg cgctacggcc atgaaggcct gcccgacgac accgtccaca
     2341 tccagtgcaa tggcacggcc ggccagagct tcggcgcttt cctggcgcac ggcatcacgc
     2401 tcgacctggt cggcgaaggc aacgactacg tgggcaaggg cctgtcgggc ggccgcatca
     2461 tcgtgcgctc gcccaacgac ttccgcggct tcggccccga gcacatcatc gccggcaaca
     2521 ccgtgctgta cggcgccctg gcgggcgagg cctacttcaa cggcgtggcc ggcgaacgct
     2581 tcgccgtgcg caactcgggc gcggccaccg tggtcgaagg caccggcgac cacggctgcg
     2641 aatacatgac gggcggcacg gtcgtggtgc tgggccacac cggccgcaac ttcgccgccg
     2701 gcatgtcggg cggcgtggcc tacgtgtggg acccggaccg cagcttccgc cagcgctgca
     2761 acctgaccat ggtcgagctc gaacccgtgc tgccgcacgc cgagcagtcg ggcaccaacg
     2821 cccaggccac ctggcacagc gcccagcgcg gcggcgagcg ccagcccgac gaaaccctcc
     2881 tgcgccgcct ggtggaagag cacttccgcc acaccggcag cttccgcgcc cgcgagatcc
     2941 tgggcgactg ggaggcctcg cgcggcaagt tcgtcaaggt catgccgacc gaatacaagc
     3001 gcgcgctggc cgaactatgg caagccgcca atcccgaaca gatggtggcg taaccatcgc
     3061 tggaacttga gatattggcg ttgtctgctc agcgggcatg gcgtgggggc gcctctctga
     3121 cgaggcagcc ccgcaggggc cgagggcccg taatcttggg cccgagcccg tcacaacgaa
     3181 gcccctacgc catgcccgct gggcagacaa cgccttaaga acacaaagga accaaaatgg
     3241 gcaagatcac cggcttcatg gaataccagc gcctgtccga ggcctacgag gccccgcaaa
     3301 agcgcctgaa gcactggagc gaattcgtcg tgcacctgac cgacgaccag gccaagcagc
     3361 aggccgcccg ctgcatggac tgcggcatcc ccttctgcaa caacggctgc ccggtcaaca
     3421 acatcatccc cgactggaac gacctggtgt atcgccagca gtggcagcag gcgctgcacg
     3481 tgctgcactc caccaacaac tttccggaat tcaccggccg catctgcccc gccccctgcg
     3541 aagccgcctg cacgctcaat atcaacagcg atgccgtcgg catcaagtcg atcgagcacg
     3601 caatcatcga caagggctgg gccgaaggct gggtccagcc gctgctgccc gcccgcaaga
     3661 ccggcaagaa agtcgccgtg gtgggctcgg gcccggccgg cctggcctgc gcccagcaac
     3721 tggcgcgcgc cggccatgcc gtgacggtgt tcgacaagag cgaccgcatc ggcggcctgc
     3781 tgcgctacgg catccccgac ttcaagctcg agaaaacgct gatcgaccgc cgcatcgccc
     3841 agatggaagc cgaaggcgtg gaattcgccc cctcgaccta catcggcaag gcttccgagg
     3901 ccgccgccga cggcctgacc gtgcgcacgc ccgaatcgct catggccgac ttcgacgccg
     3961 tggtcatggc cggcggctcg gaaaccccgc gcgacctgcc tatccccggc cgcgagctgt
     4021 ccggcatcta ctacgccatg gacttcctgc gcccgcagaa caaggccgtg gccggcgacc
     4081 gcctcgccga ccagccctcc gccgccggca agcacgtggt ggtgatcggc ggcggcgaca
     4141 cgggttcgga ctgcgtgggc accagcaacc gccacggcgc ggcctcgatc acccagttcg
     4201 agatcaacga gcaaccgcct gaaaccgaaa acaagcccct ggtgtggccc tactggccgc
     4261 tcaagctgcg cacctcgtcc tcgcacgacg aaggctgcca gcgcgactgg agcgtggtca
     4321 ccaagtcgtt ttcgggcagc aacggcaagc tcgagaagct ggtcgccgtg cacgtcgcct
     4381 gggcccgcga cgaagccacc ggccagatga agatggccga ggtccccggc tcggaattcg
     4441 agatcaaggc cgacctggtg ctgctggcca tgggcttcgt ctcgccggtg cagaccgtgc
     4501 tcgacgcctt cggcgtggac cgcgacacgc gcggcaacat ccgcgccaat accgacgact
     4561 accgcaccaa cgtcgacaag gtcttcgccg ccggcgacat gcgccgcggc cagtccctgg
     4621 tggtgtgggc catccgcgaa ggccgccagt gcgcacgctc cgtggatgag ttcctgatgg
     4681 gcagcaccga gctg
//
LOCUS       DASWPO010000246        24019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_416072, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000246 DASWPO010000000
VERSION     DASWPO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 24019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24019
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416072"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..1452
                     /locus_tag="VGC69_12800"
     CDS             475..1452
                     /locus_tag="VGC69_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEY0296203.1"
                     /translation="MNLQQFRFVRETIRRDFNLTEAARTLYTSQPGVSKAIIEFEDEL
                     GIKIFERHGKRIKGLTKPGQAVAQVIDRIMREVDNLKKVSDEYARRDEGGLVIACTHT
                     QARYLLPRVIPEFRKQFPKVRLSLAEGSPGQLAEMVLHEQADLALATESLALTPGLVT
                     LPVYSWEHTVVIRPDHPLGDLTSSEGRNLTLSQLAAYPIVTYDRAFTGRSTIDEVFSS
                     QGIHPDIVLEAIDADVIKTYVDVGLGIGIIAGVAYDPRRDNNLVGLPVGHLFGTHTTR
                     VGLKAGVFLRDYVYTFLEMLAPGLDRDTVMEALQGGSAGQAAVAAALEA"
     gene            complement(1483..2139)
                     /locus_tag="VGC69_12805"
     CDS             complement(1483..2139)
                     /locus_tag="VGC69_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY0296204.1"
                     /translation="MQATQNASRPDYPSSPLPVIWLRRAVAAGSLAVLATLAGCAGMG
                     QRSDSMIDAEEMAAHSDDPIGALIAYQYTRGRKKTGSSHSPILSEALSQLGTPYRFGG
                     ESPATGFDCSGLITYSAAHAVGLKLPRNSAALATVGTWVDRDDLRPGDLVFFDTMGSR
                     YSHVGIYMGADRFVHSPSTGGVVRIDDMTIDYWSRRYTGARRIPVRIAATQGPLQASR
                     "
     gene            2211..4112
                     /locus_tag="VGC69_12810"
     CDS             2211..4112
                     /locus_tag="VGC69_12810"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEY0296205.1"
                     /translation="METKMNKIMAFHERSIVDREGFWRREAERIHWEAPFEQVLDASR
                     LPFARWFVGGRTNLCYNAVDRWLPQLAGHPALVWVSTEVGLERVYTRKQLYDEVNAAA
                     AMLRALGVERGDRVLLYMPMVPEAVFAMLACARIGAVHSVVFGGFASVNLAQRIDDAA
                     PKVVVSTDGGSRAGKVLAYKPLLDRALALCAKPPQSVVILDRGLAPYEPVAGRDLDYA
                     ALRQRHWGQVVPVQWLESSEPSYVLYTSGTTGKPKGVQRDTGGYAVALAASMEYVFDG
                     KPGDTFFCTSDIGWVVGHSYIVYGPLIGGQTTILYEGTPVRPDGAVLWRLVERFRANT
                     LFSSPTAVRVLKRQDPALLRRHDVSSLRAVYLAGEPLDEPTARWLSDGLGKPIIDNYW
                     QTESGWPILSAQPGVERTPARLGSPSFPVYGFDARIVSESTGEDLPAGEKGVVAIVPP
                     LPPGAMSTIWGDDARFVQTYFDAIPGKRYYSTFDWGRVDEDGYWFILGRTDDVINVAG
                     HRLGTREIEESVSSHAGVAECAVVGVADELKGQVAMAFVVLKQAMQTGQTAETRALEG
                     DIMRMVEEQLGAVARPARIRFVGALPKTRSGKVLRRAIVAVCENRDPDDLTTIEDPTA
                     LEQIKESLQ"
     gene            4109..4516
                     /locus_tag="VGC69_12815"
     CDS             4109..4516
                     /locus_tag="VGC69_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0296206.1"
                     /translation="MKSKPVLGAEDVKKILAASEAHALANQWAVSIAVVDDGGHLLGM
                     LRGDGVAPISAHIAPAKARTAALGQRESKGYEDVINQGRYSFLSAPLLEGMLEGGVPV
                     VAGGQVIGAVGVSGVKSNEDAEIAKAGIAALGL"
     gene            4606..5862
                     /locus_tag="VGC69_12820"
     CDS             4606..5862
                     /locus_tag="VGC69_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296207.1"
                     /translation="MYDFANSGYSTVILTTVFSTYFVGVVGGRAAWATLAWTAALSLS
                     YLAIMLTMPTLGARADARAGKRRLLYRSTAGCVLFMLLLTRAGPGDVWLALAAVACSN
                     YCYCIGESVVAAFLPELARPHALGRVSGWGWSFGYCGGMLSLGLSLAVVSASQAHGLD
                     AQRYVPWVIMVTAAVFVLAALPSFLMLRERARPHAGPLARGFLADMRDMLARLARLWR
                     ETGVQYPQFRRLLMCCACYQAGISVVITLAAVYAEQVMGFGMAQVMLLVFTVNIAAAA
                     GAFAFGYAQDRIGHKLALAITLCGWIAMVLTAYAAVTVQVFWVAAFLAGLCMGTSQSA
                     GRAMVGVFAPAERLAEFFALWTFAVQLAAVVGPLTYGLVTWFTHGNHRLAILVTGLFF
                     VGGLALLARVRMPRRTGPPAEQCVPE"
     gene            5947..7926
                     /gene="acs"
                     /locus_tag="VGC69_12825"
     CDS             5947..7926
                     /gene="acs"
                     /locus_tag="VGC69_12825"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0296208.1"
                     /translation="MSTSIESVLVETRVFPPPASAVQGAAISGMDAYRALAEEAERDP
                     DGFWGRQARENLQWAKPFTQVLDESKAPFYRWFGDGELNVSANCLEAQIEKGNGDKVA
                     IIFEADDGKVDRITYRQLLARVSRFANGIKQLGYKQGERAIIYLPMSIEAVVAMQACA
                     RLGLTHSVVFGGFSSKSLQERIVDVGASLVITADAQMRGGKRIPLKPAVDDALAMGGC
                     EAVGKVIVYRRAGGQVDWTEGRDLWMHDVEAGQPDVCAPVPVNAEHPLFILYTSGSTG
                     KPKGVQHSSAGYLLWAMLTMKWTFDARPGDVYWCTADVGWITGHTYIAYGPLACGLTQ
                     VVFEGVPTYPDAGRFWDMIARHQVTVFYTAPTAIRSLIKACEGAPKTHPSHYALDSLR
                     ILGSVGEPINPEAWIWYHKNVGRERCPIVDTWWQTETGGHMITPLPGATPLKPGSCTL
                     PLPGIAAAIVDETGGDVEAGHGGFLVVKRPWPSMIRTIWGDPERFVKSYFPPELKGYY
                     LAGDGAQRDADGYFWIMGRIDDVLNVSGHRLGTMEVESALVAHELVAEAAVVGRPDDT
                     TGEAVVAFVVLKRARPEGDEALAIAKQLRDWVAKEIGPIAKPRDIRFGDNLPKTRSGK
                     IMRRLLRVVAKGEEVTQDVSTLENPAILEQLSKPL"
     gene            7970..8986
                     /gene="ltaE"
                     /locus_tag="VGC69_12830"
     CDS             7970..8986
                     /gene="ltaE"
                     /locus_tag="VGC69_12830"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEY0296209.1"
                     /translation="MIDLRSDTVTRPSEAMRLAMAQAPVGDDVMGDDPTVQQLQAAVA
                     ERAGKEAGLFFPSGTQSNLAALMAHCERGDEYLVGQLSHTYKYEGGGAAVLGSIQPQP
                     IEHAADGTLPLEKLQAAVKVKGDPHFANTKLLALENTFHGKVLPQDYVRAATDWAHGK
                     GLSTHLDGARVFNASVASGLSLRELCSTFDSVSICFSKGLGTPVGSVLVASQALIDKA
                     RRWRKVLGGGMRQSGILAAAGLYALAHHVDRLAQDHANAERLAAGLKGLPGVKSLSHA
                     TNMVFVEFDPAVCEGLTAALAAQGILMLASYGGPTRLVTHLDVTEADIDRAIAAAKAY
                     LMQR"
     gene            complement(9014..9811)
                     /locus_tag="VGC69_12835"
     CDS             complement(9014..9811)
                     /locus_tag="VGC69_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0296210.1"
                     /translation="MPQLQDIVRNPLKEKLRRNEIASSMMVRLVRTIEIARIIKTAGF
                     DSFYIDLEHSSLTIDAASQICMAGLELGLAPMVRVPANTPEYISRVLDGGAMGVIAPH
                     VDTAEQAARVVSYAKFAPLGERSANSGLPHLQYRNFPVAQANDALNDATMVVVQIETA
                     RALENVAEIAATPGVDMILVGTNDLLADMGLAGQYEHPALREAYGKIIAACKEAGKFV
                     GVGGLASQPKLAAEFVQRGARYVSTGTDLSFLAAACAARAKQVADLR"
     gene            complement(9828..10667)
                     /locus_tag="VGC69_12840"
     CDS             complement(9828..10667)
                     /locus_tag="VGC69_12840"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0296211.1"
                     /translation="MILPHACDTHFHVFGPAGRYPYAGKLRYTPPKADLDDYLAEARR
                     LGFTRYVFVQPSAYGQDNRCMLDAMRTMGPACRGIADLPDDTQEAKLADLHAVGVRGV
                     RINVNPIEPPTAGLSAAMLPRIARWAALCAELGWQLDFLGPGWLTAELLPTMSRLTVP
                     YTIAHLGMNLAKDGPDAPGFKALLDLLRHGDGFCHIKLTGSYRISTAPGFTDIAPMVH
                     ALLEAAPDRLIWGSDYPHLSFAENTSDALFALFKSWVPDAGLQRTILCDTPARLYGWT
                     ESN"
     gene            complement(10699..11676)
                     /locus_tag="VGC69_12845"
     CDS             complement(10699..11676)
                     /locus_tag="VGC69_12845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296212.1"
                     /translation="MHKAWAALWLAILQFALPATAATAAETAWPTHPIRLIVPFAPGS
                     FTDIAARAVSAEISSQLGQTFVVENRGGAGSIIGTDQVAKADPDGYTLLFVDNSFAVS
                     AAYYRKLPYDPAKDLTQVALVADTPAVLVCRTGLNIHTLKDLVAQARKTSGGMTFGSG
                     GQGSSAHLAMEAFLLQNGLQMVHVPFKGVAESMIDIAANRVDTGIGSIGSTVQHIQNK
                     RMVGLAISGDHRSASMPDVPTFAEAGFPDYKVRYWFGYMAPARVPAAVVERIAQAVGK
                     ALQSDRVKQVFAAAGVDPTITTPARFHALVQSDIAMWKEVIARADIQTQ"
     gene            11875..12654
                     /locus_tag="VGC69_12850"
     CDS             11875..12654
                     /locus_tag="VGC69_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0296213.1"
                     /translation="MSSLDKMLGLLDVFTPAAPVWSTEDLIRYTGVPTSTCYRYIKSL
                     HTAGFLARVGNGSYVLGPRVLELDRTMRLCDPIYIAGSPVIRELTSETGHSTLLCILF
                     SDSVMCVQEALGQHAPSQLFSRGQRRPLVAGASAKIILAHLPPHQLRVLYAKHRKAIA
                     AVGLGADWDRFRQSIKQIRQAGFVMTSGEYNPGVLSIAAPLFNRGGDVLGSLAVAASS
                     SHVDPAQFRALADRVVEAGREVSTRIATVSNAVDLPARAVG"
     gene            complement(12617..13261)
                     /locus_tag="VGC69_12855"
     CDS             complement(12617..13261)
                     /locus_tag="VGC69_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0296214.1"
                     /translation="MNAPGSSQDPAMQPQRFPAIDPGHMTARQREVADKIAGGPRGGV
                     KGPFLALLHHPELADRLQQVGEHLRFGTGMPQEVVELVILVTARHWDCQYEWYAHNRL
                     ARANTRLDPAVIEAVARDEAPAGMPAELQDAYDFCIEVHRHGQPGDRAYAAVEARFGK
                     QGVLDLLATCGYYSLLAMVLNTARIPLPEGEPPPLGPKPSSGAIPRPAPAGPPR"
     gene            complement(13258..14283)
                     /locus_tag="VGC69_12860"
     CDS             complement(13258..14283)
                     /locus_tag="VGC69_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296215.1"
                     /translation="MTSRIELYAGTAGHSAWFSEDLGQTWVHPNSHSGMYLEARVWSI
                     SSHPARPGVLYAGTDMGLFRWDEAAARWTALPSPMQDVWAVTQSADDPDVLYAGARPA
                     AFYRSADGGTTWEPLAVPGLATFSTINNGPTRVTQILVDPLDHDTLWATVEIGGIYRS
                     RDRGRNWSLQAEGLASQDVHGIAALRGPGGERILLATTNYGLHRSEDDGGHWTLQRLD
                     SPWQYTRSVVPRADTDRIVFLTNGNGPPGTDGRLLRSDDYGATWRGLPLPGRLNSTVW
                     CVATHPSDPLFIMVCTNLGQLFRSGDGGDTWERLPHEFGEVRALHWRLADIPGNRPAH
                     SITVRKS"
     gene            14387..15355
                     /locus_tag="VGC69_12865"
     CDS             14387..15355
                     /locus_tag="VGC69_12865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296216.1"
                     /translation="MRKILAWAGLCLACWIGPIGGAAAAYPEHPVHIIIGFPPGGAID
                     TIARLMAPPLSQRLGQSIIVENRAGAGGIIAAQQVVRSDPDGCTVLMGTMGNLSISPM
                     LVKDLNFDMDKDFTAVTVVASTAMVLYVNPKLPVHTVAELIAYAKANPNRVNFSSSGN
                     GGLPHMAGEMFNSATGLKLLHVPYKGSAPSVSDVVAGQVQMTIEAPAIGLPLVQTGQL
                     RALATTGSRRMAVLPDVATVAETVPGFEVMNWFGIVAPARTPADSIARLQQAIAAVLA
                     DPGLQAKLAALGVDVVANTPQEAGKYMADERVRWAKVVKDAHITIN"
     gene            15389..16939
                     /locus_tag="VGC69_12870"
     CDS             15389..16939
                     /locus_tag="VGC69_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY0296217.1"
                     /translation="MNGAESLVRTLLASNVDVCFANPGTSEMHFVAALDRVPGMRCVL
                     GLFEGVATGAADGYARMAGRPASTLLHLGPGLANGMANLHNARKARTPLLNIVGEHAS
                     DHLPYESPLRSDVPGLAALASDWYRVAADGKSVANDAAEAVRAARSRDGQIATLILPA
                     DTAWSEGAAPAVAAEPPPLAQATGESIAHVARAVRSGKRCALLATGQALGEAGLALMA
                     RYAGSSIRLLAQQSNRRMARGRGRLIVPRVPFAVDQAVEALGGLDMLVLVGSHPPAAF
                     FGYPGKPRLLAPPDCDVHTLVEPGGDALHALAWLADELGLPARTAAVESVSAPVLPGA
                     DQMLDADAAMQTMAALLPEGAILCDESVSSGRSLFRFTDAAAPHDYLQITGGSIGCGL
                     PLATGASVACPDRKVICFEGDGSGMYTLQALWTQARERLDVVTLILANRSYRILQGEL
                     KGVGIAEPGEQAHRMLDLVDPALDWVALARGQGVEAVAVETVAGLADAIKAALARRGP
                     FLIEVRLP"
     gene            complement(16940..17626)
                     /locus_tag="VGC69_12875"
     CDS             complement(16940..17626)
                     /locus_tag="VGC69_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0296218.1"
                     /translation="MRTDTGAPLKVGLMVPINNTTMESEMLGWLPPGSTCVTQRIPRG
                     QGLLTAETMPAYLAKALALAAEFPKDIDVVAYGCTAAGFIAGPEGEAKLAEQLADITG
                     KPVVTTARSMVSVLQVLGARRIALVTPYSDMVNRSLMRFLTDGDISVNRLSTFNAPDV
                     DALGRITAEEVDQRARETMATDCDALFIACSQLPTQKILAPLQRDLGRPAWSSIRATA
                     WSARQAVAAP"
     gene            complement(17739..18569)
                     /locus_tag="VGC69_12880"
     CDS             complement(17739..18569)
                     /locus_tag="VGC69_12880"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296219.1"
                     /translation="MSGKRVLLVGAGDLGLRAAQRLLAQGDEVWALRRHPLAAGHERL
                     HSLVADLGRPDTLRGLPAGITHMVWSPAPGRRDAQAYETVFTQGLRRVFAALDAAALQ
                     RVVFVSSSAVYSEHGGDWVDERTPAAPRGFNGRILLEAEQWLADQGLPAVALRLAGLY
                     GPGRTQLLDRLRAGQARAPIDPPHWANRLHVDDAASAVAHLLALPDAQPVYIGCDDTP
                     LPLHLLYGHLAGLLGAPMPMAGPAPADVGSKKLGNARLKASGWTPRWPDARDGYAHLI
                     "
     gene            complement(18655..20301)
                     /locus_tag="VGC69_12885"
     CDS             complement(18655..20301)
                     /locus_tag="VGC69_12885"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY0296220.1"
                     /translation="MSERESMEYDVVVVGGGPGGLAAAIRLKQLASERNREISVCVLE
                     KAAELGAHTLSGAVMDPQALTELIPDWQEKGAPLTVAVTQDQFLFLTQTGSRRTPGWL
                     LPACFQNHGNYIVRLGDVVKWLGEQAEALGVDVFPGFAAVEVLYDEAGAVRGVATGDM
                     CVARDGSHTDHYQPGMELLARYTLFAEGARGQLGRQLIERFKLDAGRDPQSYGIGIKE
                     LWEVDPAQSRPGLVMHTAGWPLDSDTYGGSFLYHLDNNQVAVGMVVGLDYANPWLSPY
                     DEFQRYKTHPAIRPTFEGGKRIAYGARAITAGGLLSLPKLVFPGGAMVGCEAGFLNAS
                     RIKGSHAAIKTGKMAAEAAFEAIAADRKQDELSAYPAAFESSWLHVELNKARNFKQWF
                     KKGRTVATIMTGIEQKLLGGNVPWTIHRTKPDYACLQPASQCARIDYPKPDGKITFDK
                     PSSVFISNTNHEENQPIHLTLKDASVPVAINLAQYGGPEARYCPAGVYEFVKDDHGAD
                     RLQINAQNCVHCKTCDIKDPTQNIVWTAPQGGEGPVYSGM"
     gene            20426..20899
                     /locus_tag="VGC69_12890"
     CDS             20426..20899
                     /locus_tag="VGC69_12890"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0296221.1"
                     /translation="MDTSTDQGAGQAQPAGRTLAVGGRHYLDLPLRWGDSDSMNHLNN
                     TLYFRLMEEARIQILYAAGLKLPDDQGAILAHASCDFLRPLTYPATVRVTHEVTRIGR
                     SSLEFDLILDKIGDATGSYARGRNVLVWMDYLNNRSAPWPAEVLGRLGGLFVPAG"
     gene            21015..21713
                     /locus_tag="VGC69_12895"
     CDS             21015..21713
                     /locus_tag="VGC69_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY0296222.1"
                     /translation="MNKIYANATEALAGVVKNGQMIAVGGFGLCGIPEALIAALRDSG
                     VKDLTCISNNAGVDGFGLGQLLTTRQIRKMIASYVGENKEFERQYLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGVGTIVADGKEIREFDGEQYVMERSLAPDVALVKADIA
                     DRSGNLVFRKTARNFNPNAAMAGKITIVEVEKIVETGALDPDQIHLPGIYVHRIVLNT
                     TPEKRIEQRTTRTA"
     gene            21724..22377
                     /locus_tag="VGC69_12900"
     CDS             21724..22377
                     /locus_tag="VGC69_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEY0296223.1"
                     /translation="MAWSRDEMAARAARELQDGYYVNLGIGLPTLVANHVPDGMEVWL
                     QSENGLLGIGPFPTEDEVDADLINAGKQTVTTLPGSSIFSSADSFAMIRGGKINLAIL
                     GAMQVSETGDLANWMIPGKMIKGMGGAMDLVAGVGRVVVLMEHVAKKKDGTEDLKLLP
                     ACTLPLTGVGVVDIIITDLGVLEVTPGGLKLLEVAPGVTVDEIKSKTSAKLDVSAVK"
     gene            complement(22370..22843)
                     /locus_tag="VGC69_12905"
     CDS             complement(22370..22843)
                     /locus_tag="VGC69_12905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0296224.1"
                     /translation="MPDWICKHHADLSAAELYALLQLRTAVFVLEQRCVFQDMDGQDM
                     RGETAHLMAWEDGRLAAYCRLLDPAHDEQGEAIIGRVIVAPGWRGTGLGHALMRRALA
                     EAHSRWPRAGVYLGAQAHLRGFYAAHGFVAVTQEYEEDGIPHVVMRLSHPSSRPT"
     gene            23093..>24019
                     /locus_tag="VGC69_12910"
     CDS             23093..>24019
                     /locus_tag="VGC69_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY0296225.1"
                     /translation="MPAIPTRIAALRQAMRRLGLDACVVPSSDPHLSEYLPQYWQGRR
                     WLSGFTGSVATLVVTADFAGLWVDSRYWVQAGSQLAGSGIALMKLAAPGVPGHVDWLA
                     GTLPAGARVGVDGRVLGLAGYRALTQALAPAGVALDIMADPLGEIWTDRPALPGAPVA
                     EHLPPYATESRAQRLARVRAAMAAHRADVHLLCTLDDIAWVFNLRGADVDYNPVFLAH
                     ALIGADHATLFVGEGKVSDALRARLAEDGVAVAPYEHAAEALGTIELDQTLLIDPARV
                     TCGVFHLMDPAVARIEAINPSTFFKSRKSDAEL"
BASE COUNT         3710 a         8019 c         8371 g         3919 t
ORIGIN      
        1 cacccttagg tgcgaaatgc atctattttg agatattgcc gtggctctgg tggccgcaag
       61 cgcgccgacc cgtggaaatc ggtcgtcagg taagaactct ttgtctcttg ccgttttcag
      121 gtaaacctga gactttttgg cgagatcggt aagccaggag caactaccga ccgactggcg
      181 gcgctctggc aggtcagagc atggggtgga aactcgagtg acaaagaatt cttacctagc
      241 gctataccct tgcttttcag atggataggc cattatgtta ggaaatatat cggccaccat
      301 gaggagttcc tggggatacc tggtaagaat tctttgtccc aggtaaggtt tcatctgtcg
      361 ctccacagcg gcgcgggcaa tggcgacaag attgatctat ttatattctt ataagtaata
      421 tatattatat ctcttattac ttataatgag ggtcattaaa accagggccc tgccatgaat
      481 cttcagcaat ttcgcttcgt ccgggaaacc atccggcgtg atttcaacct gaccgaagcg
      541 gcacgcacgc tctatacctc gcagcctggc gtttccaagg ccatcatcga gttcgaggac
      601 gagctgggca tcaagatctt cgagcgccat ggcaagcgca tcaaggggct gaccaagccc
      661 ggccaggcgg tggcccaggt gatcgaccgc atcatgcgcg aagtcgacaa cctcaagaag
      721 gtcagcgacg aatacgcgcg gcgcgacgag ggcgggctgg tgatcgcctg cacgcacacc
      781 caggcgcgct acctgctgcc gcgggtgatt cccgaattcc gcaagcagtt tcccaaggtg
      841 cgcctgtcgc tggccgaagg cagccccggg cagctggccg agatggtgct gcacgagcag
      901 gccgacctag ccctggccac cgagtcgctg gccctgacgc ccggcctggt gaccttgccg
      961 gtgtattcct gggagcacac ggtggtgatc cgccccgacc atcccctggg cgacctgact
     1021 tccagcgaag ggcgcaacct cacgctgtcg cagctggcgg cctatcccat cgtgacctac
     1081 gaccgcgcct tcacgggccg ctcgaccatc gacgaggtgt tctccagcca gggcatccac
     1141 cccgacatcg tgctggaagc catcgacgcc gacgtgatca agacctatgt ggacgtgggc
     1201 ctgggcatcg gcatcatcgc gggcgtggcc tacgacccgc gccgcgacaa caatctggta
     1261 ggcctgccgg tggggcattt gttcggcacg cacaccacgc gcgtcggcct gaaggccggc
     1321 gtgttcctgc gcgactacgt ctacaccttc ctggaaatgc tggcgcccgg cctggatcgc
     1381 gacaccgtga tggaagccct gcaaggcggg tcggccgggc aggccgccgt ggccgccgcc
     1441 ctcgaggcct aggcctttct caaaccctgc ggggcgcggc ggtcagcgcg aggcctgcaa
     1501 ggggccctgc gtggcggcga tccgcacggg gatgcggcgt gcgccggtat agcggcggct
     1561 ccagtagtcg atggtcatgt cgtcgatgcg gaccacgccg ccggtgctgg gcgagtggac
     1621 gaagcggtcc gcgcccatgt agatgcccac atgtgaatag cgcgagccca tggtgtcgaa
     1681 gaacaccagg tcgcccggcc gcaggtcgtc gcggtcgacc caggtgccga cggtggcgag
     1741 cgcggccgaa ttgcgcggca gcttcaggcc gacagcgtgc gcggccgaat aggtgatcaa
     1801 cccgctgcaa tcgaagcccg tggccggcga ttcgccgccg aagcggtagg gcgtgcccag
     1861 ctgcgacagg gcctcgctga ggatgggact gtgcgaagaa ccggtttttt tccggccacg
     1921 ggtgtactgg taggcgatca gcgcgccgat ggggtcgtcg gagtgggcgg ccatttcctc
     1981 ggcgtcgatc atcgaatccg aacgctggcc catgccggcg caaccggcca gcgtggccag
     2041 aacggccagg gagccggcgg ccacggcccg gcgcagccag atgacgggca gggggctgga
     2101 cgggtaatcg gggcgggagg cgttctgggt tgcttgcatg cgctcgttcg tggaaactgc
     2161 ttcgtatcga gagggatgcg ccaaggagcc gccccggcac tatatcaaaa gtggagacaa
     2221 aaatgaataa aatcatggcg ttccatgaaa gatctatagt ggatcgtgaa ggattctggc
     2281 ggcgggaggc cgagcggatc cactgggagg cgcctttcga gcaggtcctg gacgcgagcc
     2341 ggctgccgtt tgcccgctgg ttcgtgggcg ggcgcaccaa cctctgctac aacgcggtcg
     2401 accgctggct gccccagctc gccggccacc cggcgctggt ctgggtatcg accgaggtcg
     2461 gcctggagcg cgtctatacc cgcaagcagc tgtacgacga ggtcaatgcc gcggccgcca
     2521 tgctgcgcgc gctgggcgtg gaacgcggcg accgggtgct gctctacatg cccatggtgc
     2581 ccgaggcggt gttcgccatg ctggcctgcg cccgcatcgg cgccgtgcat tcggtggtgt
     2641 tcggcggctt cgcctcggtg aacctggcgc agcgcatcga cgacgcggcg cccaaggtgg
     2701 tggtgagcac cgacggcggc tcgcgcgccg gcaaggtgct ggcctacaag cccctgctcg
     2761 accgcgcatt ggcgctatgc gccaagccgc cgcagtcggt ggtgatcctc gaccgcggcc
     2821 tggccccgta cgagcccgtg gccgggcgcg acctcgacta cgccgccctg cgccagcggc
     2881 attgggggca ggtggtgccg gtgcaatggc tggagtcttc cgagcccagc tatgtgctgt
     2941 acacctcggg caccacgggc aaacccaagg gcgtgcagcg cgatacgggc ggctatgcgg
     3001 tggcgctggc cgcttccatg gaatacgtct tcgacggcaa gccgggcgac actttcttct
     3061 gcaccagcga cataggctgg gtggtggggc attcgtacat cgtctacggc ccgctcatcg
     3121 gcgggcagac caccatcctg tacgaaggca cgccggtacg ccccgacggc gcggtgctgt
     3181 ggcggctggt ggagcgcttt cgcgccaaca cgctgttttc ctcgcccacg gcggtgcgcg
     3241 tgctcaagcg ccaggatccg gccttgctca ggcgccatga cgtgtcgtcg ctgcgggcgg
     3301 tctacctggc gggcgaaccg ctggacgaac ccacggcgcg ctggctgtcc gacgggctgg
     3361 gcaagcccat catcgacaac tactggcaga ccgaatcggg ctggcccatc ctgtcggcgc
     3421 agcccggcgt ggaacgcacg ccggcgcgct tgggcagccc gtcctttccg gtatacggct
     3481 tcgatgcccg catcgtgagc gaatccacgg gcgaggacct gccggcgggc gagaagggcg
     3541 tggtggccat cgtgccgccg ctgccgccgg gggccatgtc caccatctgg ggcgacgacg
     3601 cgcgcttcgt gcagacctat ttcgacgcca ttccgggcaa gcggtattac tcgaccttcg
     3661 actgggggcg ggtggacgag gacggctact ggttcatcct gggccgcacc gacgacgtga
     3721 tcaacgtggc cgggcaccgg ctgggcacgc gcgaaatcga ggagtccgtc agcagccatg
     3781 ccggcgtggc cgaatgcgcg gtggtcggcg tggccgacga gctcaagggc caggtggcca
     3841 tggccttcgt ggtgctcaag caggcgatgc aaactggtca gaccgcggaa acccgggcgc
     3901 tggaaggcga tatcatgcgc atggtggaag aacaactcgg cgccgtggca cggcccgccc
     3961 gcatccgttt cgtgggcgcc ttgcccaaga cgcgttccgg caaggtgctg cggcgggcca
     4021 tcgtggccgt gtgcgaaaac cgcgacccgg acgatctcac caccatcgaa gaccctacag
     4081 cgcttgaaca gatcaaggaa tcactgcaat gaagagcaaa cccgtgctgg gcgcggaaga
     4141 cgtgaagaaa atcctggcgg cttccgaggc gcatgccctg gccaaccagt gggccgtgag
     4201 cattgccgtc gtcgacgacg gcggccatct gctgggcatg ctgcgcggcg acggcgtggc
     4261 ccccatctcg gcgcacatcg cgccggccaa ggcgcgcacg gcggcgctgg gccagcgcga
     4321 gtcgaaaggc tatgaagacg tcatcaacca gggccgctat tccttcctgt cggcgccgct
     4381 gctcgagggc atgctcgaag gcggcgtgcc cgtggtggcc ggcggccagg tgatcggcgc
     4441 cgtgggcgtc tcgggcgtga agtcgaacga agacgccgag attgccaagg ccggcatcgc
     4501 cgcgctcggc ctgtgagttc cgcttcgccg gccggcgccg tacccggcgg ggccggcggg
     4561 gcgctcaatc ccggggtgac ccggcgcgag gtctgggcct gggcgatgta cgacttcgcc
     4621 aactcgggct acagcaccgt catcctcacc acggtattca gcacgtattt cgtcggcgtg
     4681 gtgggcggca gggccgcctg ggccacgctg gcctggacgg cggcgctgtc gctgtcgtat
     4741 ctcgccatca tgctcaccat gcccacgctg ggcgcgcgcg ccgacgcgcg cgcgggcaag
     4801 cgccggctgc tctaccgcag caccgcgggc tgcgtgctct tcatgctgct gctcacgcgg
     4861 gccgggccgg gcgatgtctg gctggccctg gcggcggtgg cctgttccaa ctactgctat
     4921 tgcatcggcg agtccgtggt ggcggccttc ctgccggaac tggcgcggcc ccatgccctg
     4981 gggcgcgtgt ccggctgggg ctggagcttc ggctattgcg gcggcatgct cagcctgggg
     5041 ctgtcgctgg cggtggtctc ggccagccag gcgcatggcc tggacgcgca gcgctacgtg
     5101 ccctgggtca tcatggtgac ggcggcggtg ttcgtgctgg cggcgctgcc gtccttcctg
     5161 atgctgcgcg agcgcgcgcg gccgcacgct ggcccgctgg cgcgcggctt cctggcggac
     5221 atgcgcgaca tgctggcgcg cctggcgcgc ttgtggcgcg aaaccggcgt ccaatacccg
     5281 caattccgcc ggctgctgat gtgctgcgcc tgctaccagg cgggcatctc ggtggtcatc
     5341 acgctggcgg cggtctatgc cgagcaggtc atgggcttcg gcatggcgca agtcatgctg
     5401 ctggtgttca ccgtcaatat cgcggccgca gcgggcgcct tcgccttcgg ctacgcgcag
     5461 gaccgcatcg gccacaagct cgccctggcg atcaccctgt gcggctggat cgccatggtg
     5521 ctgacggcct atgcggccgt cacggtgcag gtgttctggg tggcggcctt cctggcgggc
     5581 ctgtgcatgg gcaccagcca gagcgcgggc cgggccatgg tcggcgtgtt cgcgccggcc
     5641 gaacggctgg ccgagttctt cgccttgtgg acgttcgcgg tgcaactggc cgcggtggtg
     5701 gggccgctga cctacggcct ggtgacctgg ttcacgcacg gcaaccaccg cctggccatc
     5761 ctggtgacgg ggctcttctt cgtgggcggc ctggccttgc tggcgcgcgt gcggatgccg
     5821 cgccggaccg gaccgccggc ggaacaatgc gttccagagt aaggtgtccg taagagcgca
     5881 gcgatacggt gccgaggttg tataaataaa aacagctgca tcaaattttt cgtacggaga
     5941 caaaccatgt ccacctccat tgagtccgtc ctggtcgaaa cccgcgtatt cccgccgccc
     6001 gcgagcgcgg tgcagggcgc ggccatttcg ggcatggatg cctaccgcgc gctggccgaa
     6061 gaggccgagc gcgaccccga cggcttctgg ggccgccagg cgcgcgagaa cctgcagtgg
     6121 gccaagccct tcacccaggt gctcgacgaa tccaaggcgc cgttctaccg ctggttcggc
     6181 gacggcgagc tcaacgtctc ggccaactgc ctcgaggcgc agatcgagaa gggcaacggc
     6241 gacaaggtcg ccatcatctt cgaggccgac gacggcaagg tcgaccggat cacctaccgc
     6301 cagctcctgg cgcgcgtgag ccgcttcgcc aatggcatca agcagctggg ctacaagcag
     6361 ggcgagcgcg ccatcatcta cctgcccatg tccatcgagg ccgtggtcgc catgcaggcc
     6421 tgcgcccgcc tggggctgac gcactcggtg gtgttcggcg gcttttcgtc caagagcctg
     6481 caggagcgca tcgtcgacgt gggcgcttcg ctggtcatca cggccgacgc gcagatgcgc
     6541 ggcggcaagc ggatcccgct caagcccgcc gtggacgacg ccctggccat gggcggctgc
     6601 gaggccgtgg gcaaggtcat cgtctatcgc cgcgccggcg gccaggtcga ctggaccgaa
     6661 ggccgcgacc tgtggatgca cgacgtcgaa gccggccagc ccgacgtgtg cgcgccggtg
     6721 ccggtcaatg ccgagcaccc gctcttcatc ctctacacct ccggctccac cggcaagccc
     6781 aagggcgtgc agcattcctc ggcgggctac ctgctgtggg ccatgctcac catgaagtgg
     6841 accttcgatg cgcgccccgg cgacgtctac tggtgcaccg ccgacgtggg ctggatcacc
     6901 ggccacacct atatcgccta cgggcccttg gcctgcggcc tgacgcaggt ggtgttcgag
     6961 ggcgtgccca cctatcccga tgccggccgc ttctgggaca tgatcgcgcg ccaccaggtc
     7021 acggtgttct acaccgcgcc caccgccatc cgctcgctca tcaaggcctg cgaaggcgcg
     7081 cccaagacgc acccgagcca ctacgcgctc gattccctgc gcatcctggg ctcggtgggc
     7141 gagcccatca accccgaggc ctggatctgg tatcacaaga acgtcggccg cgagcgctgc
     7201 cccatcgtcg acacctggtg gcagaccgag accggcggcc acatgatcac cccgctgccc
     7261 ggcgccacgc cgctcaagcc gggatcgtgc acgctgccgc tgcccggcat cgccgcggcc
     7321 atcgtcgatg aaaccggcgg cgacgtcgag gcgggccacg gcggcttcct ggtcgtcaag
     7381 cgcccctggc cctcgatgat ccgcaccatc tggggcgacc ccgagcgctt cgtcaagagc
     7441 tacttcccgc ccgagctcaa gggctattac ctggcgggcg acggcgccca gcgcgacgcc
     7501 gacggctact tctggatcat gggccgcatc gacgacgtgc tcaacgtctc gggccaccgc
     7561 ctgggcacca tggaggtcga gtccgccctg gtggcgcacg aactggtggc cgaggccgcc
     7621 gtggtgggac gccccgacga caccaccggc gaggccgtgg tggccttcgt ggtgctcaag
     7681 cgcgcccgtc ccgagggcga cgaggccctg gccatcgcca agcagctgcg cgactgggtc
     7741 gccaaggaaa tcggccccat cgccaagccc agggacatcc gcttcggcga caacctgccc
     7801 aagacccgct cgggcaagat catgcgccgc ctgctgcgtg tggtcgccaa gggcgaagag
     7861 gtcacgcagg acgtttccac gctggaaaat ccggcgatcc tggagcaact gtccaagccg
     7921 ctgtagtgct aaagtcatcg gattcttcac gcaatatccg gagcatccga tgatcgacct
     7981 gcgcagcgac accgtcaccc gtccttccga ggccatgcgc ctggccatgg cccaggcccc
     8041 ggtgggcgac gatgtcatgg gcgacgaccc caccgtgcag cagctgcagg ccgcggtcgc
     8101 cgagcgcgcc ggcaaagagg ccggcctctt tttcccctcg ggcacccaaa gcaacctcgc
     8161 cgccctgatg gcgcactgcg agcgaggcga cgaatacctg gtcggccagc tctcgcacac
     8221 ctacaaatac gaaggcggcg gcgccgccgt gctgggcagc atccagcccc agcccatcga
     8281 gcatgccgcc gacggcacgc tgccgctgga aaagctgcag gccgccgtca aggtcaaggg
     8341 cgacccgcac ttcgccaaca ccaagctgct ggccctggaa aacactttcc acggcaaggt
     8401 cctgccccag gactacgtgc gcgccgccac cgactgggcg catggcaagg gcctgtccac
     8461 ccacctcgac ggcgcgcgcg tcttcaacgc ctcggtcgcc agcggcctgt ccctgcgcga
     8521 gctgtgctcg accttcgaca gcgtgtcgat ctgcttctcc aagggcctgg gcacgccggt
     8581 cggctcggtt ctggtggcca gccaagccct gatcgacaag gcgcgccgct ggcgcaaggt
     8641 gctgggcggc ggcatgcggc agtcgggcat cctggccgcc gccggcctgt acgcgctggc
     8701 gcatcacgtc gaccgcctgg cgcaggacca cgccaatgcc gagcgcctgg ccgccggctt
     8761 gaagggcctg ccgggcgtga agtcgctgtc gcacgccacg aacatggtct tcgtcgaatt
     8821 cgaccccgcg gtctgcgaag gcctcaccgc cgcgctggcc gcgcaaggca tcttgatgct
     8881 ggccagctac ggcgggccca cgcgcctggt cacccacctg gacgtgaccg aggcggacat
     8941 cgaccgcgcc atcgcggccg ccaaggcgta tctcatgcag cgttgatcag gaccgcatgg
     9001 ccggcccgcg cgttcaacgc aggtcggcca cctgcttggc gcgcgccgcg caggcggcgg
     9061 ccaggaagct gaggtcggtc ccggtcgaga cgtagcgggc tccccgctgc acgaattccg
     9121 cggccagctt gggctgcgac gccaggccgc cgaccccgac gaacttgccg gcttctttgc
     9181 aggcggcgat gatcttgccg taggcttcgc gcaatgccgg gtgctcgtac tgcccggcca
     9241 ggcccatgtc ggccagcagg tcgttggtgc cgaccaggat catgtccacc cccggcgtgg
     9301 cggcgatctc cgccacgttt tccagcgcgc gggccgtttc gatctgcacc accaccatgg
     9361 tggcgtcgtt caaggcgtcg ttcgcctgcg cgaccgggaa gttgcggtac tgcaggtgcg
     9421 gcagtcccga gttggccgag cgttcgccca gcggcgcgaa cttggcatag gacacgacgc
     9481 gcgcggcttg ctcggcggtg tcgacgtgcg gcgcgatcac gcccatcgcg ccgccgtcga
     9541 gcacgcgcga gatgtattcg ggcgtgttgg ccggcacgcg caccatgggc gccagcccca
     9601 gttccagtcc ggccatgcag atttgcgacg ccgcgtcgat ggtgaggctg ctgtgctcca
     9661 ggtcgatgta gaacgagtcg aagccggcgg tcttgatgat gcgggcgatt tcgatggtgc
     9721 gcaccagccg caccatcatc gacgacgcga tttcgttgcg gcgcagtttt tccttgagcg
     9781 gattgcgcac gatgtcttgc agttgcggca tgatgcctcc cggtgggtca gttcgattcg
     9841 gtccagccgt acagccgggc cggcgtgtcg cacaggatgg tgcgctgcag cccggcgtcc
     9901 ggcacccatg acttgaacag cgcgaacagc gcgtccgacg tgttttcggc gaaggacagg
     9961 tgcgggtagt cgctgcccca gatcaggcgg tcgggcgcgg cttccagcag cgcgtgcacc
    10021 atgggcgcga tatcggtgaa gcccggggcc gtggaaatgc ggtagctgcc ggtgagcttg
    10081 atgtggcaga agccgtcgcc gtggcgcagc aggtccagca gcgccttgaa gccgggggcg
    10141 tcggggccgt ccttggccag gttcatgccc aggtgcgcga tggtgtaggg cacggtcagc
    10201 cggctcatgg tgggcagcag ctcggcggtc agccagccgg ggcccaggaa gtccaactgc
    10261 cagcccagtt cggcgcagag cgccgcccag cgcgcgatgc gcggcagcat ggcggcgctc
    10321 agcccggcgg tcggcggctc gatggggttg acgttgatgc gcacgccgcg cacgcccacc
    10381 gcgtgcaggt cggccagctt ggcttcctgc gtgtcgtccg gcagatcggc gatgccgcgg
    10441 caggccgggc ccatggtgcg catggcgtcg agcatgcagc ggttgtcctg cccgtaggcg
    10501 ctgggctgca cgaacacgta gcgcgtgaag cccaggcgcc gcgcctcggc caggtagtcg
    10561 tcgagatcgg ccttgggcgg cgtgtagcgc agcttgcccg cgtaggggta gcggccggcg
    10621 ggcccgaaaa cgtggaagtg cgtgtcgcag gcgtggggca ggatcatggc gcttcttgta
    10681 tgggttgggg aagaaaagtc attgcgtctg gatgtccgcc cgggcgatga cctctttcca
    10741 catggcgatg tcgctctgca cgagggcgtg gaacctggcc ggcgtggtga tggtcgggtc
    10801 gacgccggcc gccgcgaaca cctgcttgac ccggtcggat tgcagggcct tgcccacggc
    10861 ctgcgcgatg cgctcgacca cggcggcggg cacccgggcg ggcgccatgt agccgaacca
    10921 gtagcgcacc ttgtagtccg gaaagccggc ctcggcgaag gtaggcacgt ccggcatcga
    10981 tgcgctgcgg tgatcgccgc tgatggccag gccgaccatc cgcttgttct ggatgtgctg
    11041 cacggtcgag ccgatgctgc cgatgccggt gtcgacccgg ttggccgcga tgtctatcat
    11101 cgattccgcc acgcccttga acggcacgtg gaccatctgc aggccgttct gcagcaggaa
    11161 ggcctccatg gcgaggtggg ccgaagagcc ctgtccgccg gagccgaaag tcattccgcc
    11221 cgaggtcttg cgggcctgcg cgaccaggtc cttcaaggtg tggatgttca ggcccgtgcg
    11281 gcagaccagc acggccggcg tgtcggcgac cagggcgacc tgcgtcaggt ccttggccgg
    11341 gtcgtagggc agcttgcggt agtaggccgc cgacacggcg aaggaattgt ccacgaacag
    11401 cagggtatag ccgtcgggat cggccttggc cacctggtcc gtgccgatga tgctgccggc
    11461 cccgccgcgg ttctcgacca cgaaggtctg gcccagctgc gaggagatct cggccgacac
    11521 cgcccgggcc gcgatgtcgg tgaaggagcc gggcgcgaag ggcacgatca gccggatcgg
    11581 atgcgtgggc cacgccgttt cggcggccgt ggccgccgtt gcgggtaagg cgaactgtag
    11641 aatggccagc catagggcag cccaggcctt gtgcatgatt tgtctccggc gggccgtcat
    11701 tgttttgagc aagagcagat acgagccgcg cctgcgtaag ggtgacgccg acggcggccc
    11761 attgccggca ggttgttgga attctggcat gcaaaatata attttgtcta gcaatatgag
    11821 aatttttaga ttcctggctc ttgcaactcc cgcaacccga ggcagcctct tcctatgagt
    11881 agcctggaca agatgcttgg cctgctggat gtcttcacgc cagcggctcc cgtatggtcg
    11941 accgaggacc tgatccgcta caccggcgtg cccacctcga cctgctaccg ctacatcaag
    12001 tcgctgcaca cggcgggatt cctggcgcgg gtgggcaatg gttcgtacgt gctgggaccg
    12061 cgcgtgctcg agctggaccg caccatgcgc ttgtgcgatc cgatctatat cgcggggtcg
    12121 ccggtgatcc gcgaactcac cagcgaaacc gggcacagca cgctgctatg catcctgttc
    12181 agcgattccg tcatgtgcgt gcaggaagcg ctggggcagc acgcgccctc gcagctgttc
    12241 agccgcggcc agcgcaggcc gctggtcgcc ggcgcgtcgg ccaagatcat cctggcgcac
    12301 ttgccgccgc atcaattgcg ggtgctctac gccaagcacc gcaaggcgat cgcggcggtc
    12361 ggcctggggg ccgattggga ccgtttcagg cagtccatca agcagatccg ccaggccggc
    12421 ttcgtgatga cctcgggcga atacaacccg ggcgtgctgt ccatcgccgc gccgctcttt
    12481 aaccgcggcg gcgacgtgct gggcagcctg gcggtcgcgg cctcgtcgtc gcacgtggac
    12541 ccggcgcagt ttcgcgcgct ggccgaccgg gtcgtcgagg ccggccggga agtctcgacg
    12601 cggatcgcga ccgtttctaa cgcggtggac ctgccggcgc gggccgtggg atagcgccgc
    12661 tggacggctt cggtcccagc ggcgggggct cgccttcggg caaggggatg cgcgcggtgt
    12721 tgagcaccat ggccagcagg ctgtagtagc cgcaggtggc gagcaggtcc agcacgccct
    12781 gcttgccaaa gcgcgcctcg acggccgcat aggcgcggtc gccgggctgg ccgtgccggt
    12841 ggacctcgat gcagaagtca taggcgtcct gcagttcggc gggcatgccg gcgggcgcct
    12901 catcgcgggc gacggcctcg atgacggccg ggtcgaggcg ggtgttggcg cgcgccaggc
    12961 ggttgtgggc gtaccactcg tactggcagt cccagtgccg cgcggtgacc aggatgacga
    13021 gttcgaccac ttcctgcggc atgcccgtgc cgaagcgcag atgttcgccc acctgctgca
    13081 ggcggtcggc cagttcgggg tggtgcagca gggcgaggaa gggccccttc acgccgcccc
    13141 gggggccgcc ggcgatcttg tcggcgactt cccgctgccg cgcggtcatg tgtccggggt
    13201 cgatggcggg aaagcgctgg ggctgcatgg cggggtcctg ggaggagccg ggcgcgttca
    13261 tgatttgcgc accgtgatgg aatgggcggg gcggttcccg gggatgtcgg ccagccgcca
    13321 atgcagcgcg cgcacttcgc cgaactcgtg cggcagccgt tcccaggtgt cgccgccgtc
    13381 gccgctgcga aacagctggc cgaggttggt gcacaccatg atgaacagcg gatccgacgg
    13441 gtgcgtggcc acgcaccaga ccgtgctgtt caggcgcccc ggcaggggca gcccccgcca
    13501 ggtggcgccg tagtcgtcgc tgcgcagcag gcgcccgtcg gtgccgggcg ggccgttgcc
    13561 gttggtgaga aacacgatgc ggtcggtatc ggcgcgcggc acgaccgagc gcgtgtattg
    13621 ccacggggaa tcgagccgct gcaaggtcca gtgcccgccg tcgtcctcgc tgcggtgcag
    13681 cccgtagttc gtcgtggcca gcaggatgcg ctcgcccccg gggccgcgca gggcggcgat
    13741 gccgtgcacg tcctgcgagg ccaggccctc ggcctggagc gaccagttgc ggccgcggtc
    13801 gcggctgcgg tagatgccgc cgatctccac ggtggcccag agcgtatcgt ggtccagcgg
    13861 gtcgaccaat atctgcgtga cccgggtagg gccgttgttg atggtggaga aggtggccag
    13921 gccgggcacg gccagcggct cccaggtcgt cccgccgtct gccgaacggt agaaggcggc
    13981 cggcctggcg ccggcgtaga ggacgtcggg atcgtcggcc gactgcgtga cggcccagac
    14041 gtcctgcatg ggcgagggca gggccgtcca gcgcgcggcg gcctcgtccc agcggaacag
    14101 gcccatgtcg gtgccggcat acagcacgcc gggacgggcg gggtggctgc tgatcgacca
    14161 gacgcgcgcc tccaggtaca tgcctgaatg actgttgggg tgcacccagg tctggccgag
    14221 gtcttcgctg aaccaggcgg aatggcctgc cgtgcctgcg tagagttcga tccggctcgt
    14281 catgcggaat gtctcctgtc ggtttttttg tcgtcttgtt ttaaaaatat tctactatat
    14341 ggaaaatttt taataaataa gacgtgaacc agaggaggag acaatcatga ggaaaatact
    14401 tgcctgggcc ggcctgtgcc tggcctgctg gatcggcccg atcggcggcg cggccgccgc
    14461 ttatcccgag cacccggtgc acatcatcat cggcttcccg cccggagggg cgatcgacac
    14521 catcgcgcgg ctgatggcgc cgcccctgtc gcaacggctg ggccagtcca tcatcgtcga
    14581 gaaccgcgcc ggcgccggcg gcatcatcgc cgcccagcag gtggtgcggt ccgatcccga
    14641 cggctgcacc gtgctgatgg gcaccatggg caacctgagc atctcgccca tgctggtgaa
    14701 ggacctgaac ttcgacatgg acaaggattt caccgcggtc acggtggtcg cctccacggc
    14761 catggtgctg tacgtgaatc ccaagctgcc ggtgcacacg gtggccgaac tgatcgccta
    14821 cgccaaggcc aatccgaacc gggtcaattt ttcctcgagc ggcaacggcg ggctgcccca
    14881 catggccggc gagatgttca actcggccac cgggctcaag ctgctgcacg tgccctacaa
    14941 gggcagcgcg cccagcgtca gcgatgtcgt cgccggacag gtgcagatga ccatcgaggc
    15001 gcccgccatc ggcctgcccc tggtgcagac gggccagttg cgcgcgctgg cgacgacggg
    15061 cagccgccgc atggccgtgc tgcccgacgt ggcgacggtg gccgagaccg tgcccggctt
    15121 cgaggtcatg aactggttcg gcatcgtcgc gccggccagg acgccggccg acagcatcgc
    15181 gcggctgcag caggccatcg ccgcggtgtt ggccgacccc ggcctgcagg ccaagctcgc
    15241 ggcgctcggc gtcgacgtgg tggccaatac gccgcaggaa gcgggaaaat acatggcgga
    15301 cgagcgcgtg cgctgggcca aggtggtcaa ggacgcgcac atcacgatca actaggactc
    15361 acctctggat ggaaggcaac gcactatgat gaacggcgct gaatcactgg tccgcacgct
    15421 gctcgcgtcg aacgtcgacg tctgcttcgc caacccgggc acgtcggaaa tgcatttcgt
    15481 ggcggcgctg gaccgcgtgc cgggcatgcg atgcgtgctg ggcctgttcg agggcgtggc
    15541 cacgggcgcc gcggacggtt acgcgcgcat ggcgggccgg cccgcctcga ccctgctgca
    15601 cctcggtccg gggctggcca acggcatggc caacctgcac aacgcccgca aggcgcgcac
    15661 gccgctgctc aacatcgtgg gcgagcacgc cagcgaccac ctgccctacg aaagcccgct
    15721 gcgcagcgat gtgcccggcc tcgcggccct ggcctcggac tggtaccggg tggcggccga
    15781 cggcaagagc gtcgcgaacg atgcggccga agcggtgcgg gcggcgcgca gccgcgacgg
    15841 ccagatcgcc accctgatcc tgcccgccga cacggcctgg agcgaggggg ccgcgccggc
    15901 tgtcgccgcc gagccgccgc ccctggcgca agccaccggc gagtcgattg cccacgtggc
    15961 gcgcgccgtg cgcagcggca agcgttgcgc cctgctggcg accgggcagg cgctcggcga
    16021 agccgggctc gcgctcatgg cgcgctatgc gggctcgagc atccggctgc tggctcagca
    16081 atcgaaccgc aggatggcgc gcgggcgcgg gcggctgatc gtgccgcgcg tgccgttcgc
    16141 ggtcgaccag gcggtcgagg cgctgggcgg gctggacatg ctcgtgctgg tgggcagcca
    16201 tccgcccgcc gcgttcttcg gctatccagg caagcccagg ctgctggctc cgcccgattg
    16261 cgacgtccac acgctggtcg agcccggcgg cgacgcgctg cacgcgctgg cctggctggc
    16321 cgacgaactg ggcctgccgg cgcgcacggc ggccgtcgag tccgtgtccg cgccggtgct
    16381 gccgggcgcc gaccagatgc tggatgccga tgccgccatg cagaccatgg ccgcgctgct
    16441 gccggaaggc gccattctct gcgacgaatc ggtttcgtcg ggacgttcgc tgttccgctt
    16501 caccgacgcc gcggcgccgc acgattacct gcagatcacg ggcgggtcga taggctgcgg
    16561 cctgcccctg gccaccggcg cgagcgtggc ctgcccggac cgcaaggtga tctgcttcga
    16621 gggcgacggc agcggcatgt acacgctgca ggcgctgtgg acgcaggcgc gcgagcggct
    16681 cgacgtcgtc acgctgatcc tggcgaaccg cagctatcgc atcctgcagg gcgagctcaa
    16741 gggcgtgggc atcgccgagc cgggcgagca ggcccatcgc atgctggatc tcgtcgaccc
    16801 ggcgctggac tgggtggcgc tggcgcgcgg ccagggcgtc gaggccgtgg cggtggagac
    16861 ggtggcgggc ctggccgatg ccatcaaggc ggcgctggcg cggcgcgggc ccttcctgat
    16921 cgaggtgcgc ctgccctgat cagggcgcgg cgacggcctg cctggccgac caggccgtcg
    16981 cgcgtatcga ggaccaggcc ggccggccca ggtcgcgctg caggggggcg agtattttct
    17041 gcgtaggcaa ttgcgagcag gcgatgaaca gggcatcgca gtcggtggcc atggtctcgc
    17101 gggcgcgctg gtccacctct tcggcggtga tgcggcccag cgcgtcgacg tcgggcgcgt
    17161 tgaaggtgct gagccggttg accgaaatgt cgccgtcggt gagaaagcgc atcagcgagc
    17221 ggttcaccat gtcgctgtag ggggtgacca gggcgatgcg ccgggcgccc agcacttgca
    17281 gcaccgagac catcgagcgc gccgtggtca cgacgggctt gccggtgatg tcggccaatt
    17341 gttcggccag cttcgcctcg ccttccggac ccgcgatgaa accggccgcg gtgcagccgt
    17401 aggccacgac gtcgatgtcc ttggggaatt cggccgccag cgccagcgcc ttggccaggt
    17461 aggccggcat ggtctcggcc gtcagcaggc cttggccgcg cgggatgcgc tgggtgacgc
    17521 aggtcgagcc cggcggcagc cagccgagca tctcgctttc catggtggtg ttgttgatcg
    17581 gcaccatgag tccgaccttc aggggcgcgc cggtatcggt tcgcatgctt gtctccttgc
    17641 ccgtctattt gcgggcgcgt tttgcaggca cgcctagcgt gcgcacccta tgttctggat
    17701 ttcataataa aacttatttc caccatttgg aaaaatattt atatcagatg cgcgtacccg
    17761 tcgcgcgcgt ccggccagcg cggcgtccag ccgctggcct tcagccgggc gttgccgagt
    17821 ttcttgctgc ccacatcggc cggcgccggg cccgccatcg gcatcggcgc gcccaggagg
    17881 ccggccagat gcccgtacag caagtgcagg ggcagcggcg tgtcgtcgca gccgatatag
    17941 accggctgcg catccggcag ggccagcagg tgcgcgacgg ctgacgccgc atcgtccacg
    18001 tgcaggcggt tggcccagtg cggcggatcg atgggcgcgc gggcctggcc ggcgcgcagc
    18061 cgatccagca attgagtgcg gcccgggccg tagagcccgg ccagccgcag cgccacggcg
    18121 ggcagcccct ggtccgccag ccactgctcc gcctcgagca ggatgcgtcc gttaaagccc
    18181 cgcggggcgg cgggcgtgcg ctcatcgacc cagtcgccgc catgctcgct atagacggcc
    18241 gacgaggaaa cgaacaccac gcgctgcagc gccgccgcgt ccagtgcggc gaacacgcgc
    18301 cgcaagccct gcgtgaagac ggtttcatag gcttgggcgt cgcgccggcc gggggcgggc
    18361 gaccagacca tatgcgtgat gccggccggc aagccccgca aggtgtcggg cctgccgagg
    18421 tcggcgacaa ggctatgcag gcgctcatgc cctgccgcca ggggatgccg gcgcagggcc
    18481 cagacttcgt cgccttgcgc gagcagccgc tgcgcggcgc gcaggcccag atcgcccgcg
    18541 ccgaccagca atacgcgttt gccgctcatt tgcgctcctt gtgaaaaagg cgccggggac
    18601 cgcgatcccg ggcgccttcg ccgctgccgc ccccggcagc gggggttgca caggctacat
    18661 gccgctgtag accgggcctt cgccgccctg cggggccgtc cacacgatgt tctgcgtggg
    18721 gtccttgatg tcgcaggtct tgcagtgcac gcagttctgc gcgttgatct gcaggcggtc
    18781 ggcgccgtgg tcgtccttga cgaattcgta cacgccggcc ggacagtagc gcgcttccgg
    18841 gccgccgtac tgggccaggt tgatcgccac cggcaccgac gcatccttga gcgtgagatg
    18901 gatgggctgg ttctcttcgt ggttggtgtt cgagatgaac accgaactgg gcttgtcgaa
    18961 ggtgatcttg ccgtccggct tgggatagtc gatgcgcgcg cactgcgagg cgggctgcag
    19021 gcaggcgtag tcgggcttgg tgcggtggat ggtccagggc acattgccgc cgagcagctt
    19081 ctgctcgatg ccggtcatga tcgtggcgac ggtgcggccc ttcttgaacc attgcttgaa
    19141 attgcgggcc ttgttcagct cgacgtgcag ccacgaggac tcgaaggcgg ccgggtaggc
    19201 cgacagctcg tcctgcttgc ggtcggcggc gatggcctcg aaggcggcct cggcggccat
    19261 cttgccggtc ttgatggcgg catggctgcc cttgatgcgc gaggcgttca ggaagccggc
    19321 ctcgcagccc accatggcgc cgccggggaa gaccagcttg ggcagcgaca gcaggccgcc
    19381 ggccgtgatg gcgcgcgcgc cgtaggcgat gcgcttgcca ccctcgaagg tggggcggat
    19441 ggccgggtgc gtcttgtagc gctggaattc gtcgtagggc gacagccagg ggttggcgta
    19501 gtccaggccc accaccatgc ccacggccac ctggttgttg tccaggtggt agaggaagga
    19561 gccgccgtag gtgtcggaat ccagcggcca gccggcggtg tgcatcacca ggccgggcct
    19621 ggactgcgcc gggtcgactt cccagagttc cttgatgccg atgccgtagc tttgcgggtc
    19681 gcggccggcg tcgagcttga agcgttcgat cagttggcgg cccagctggc cgcgcgcgcc
    19741 ttcggcgaag agggtgtagc gcgccagcag ttccatgccg ggctggtaat ggtcggtgtg
    19801 cgagccgtcg cgcgccacgc acatgtcgcc cgtggccacg ccgcgcacgg cgcctgcttc
    19861 gtcgtacagc acttccacgg cggcaaagcc ggggaacacg tccacgccca gcgcctcggc
    19921 ctgttcgccc agccacttga ccacgtcgcc caggcgcacg atgtaattgc cgtggttctg
    19981 gaagcaggcg ggcagcagcc agccgggcgt gcggcgcgag ccggtctggg tgaggaacag
    20041 gaactggtcc tgcgtgaccg ccacggtgag cggcgcgcct ttttcctgcc agtcggggat
    20101 aagctcggtg agcgcctgcg ggtccatcac ggcgccggac agcgtgtggg cgcccagttc
    20161 ggcggccttt tccagcacgc agacgctgat ctcgcggttg cgctcgctgg cgagctgttt
    20221 caatcggatg gccgcggcca ggccgcctgg gcccccgccc acgacgacta cgtcgtattc
    20281 catcgattcg cgttctgaca tgtttttgtc ccccctctcg gtgtgcagcg gtagcgcccc
    20341 ggttgccgtg ccccgggacc ggcctgtcgc caggcggttt atcatcgcat cgaagtatag
    20401 aggagactgg gaaatggacc gaggaatgga cacaagcacg gaccagggcg cgggccaggc
    20461 ccagccggcc ggccgcacgc tggccgtggg cggccggcac tacctggacc tgcccctgcg
    20521 ctggggcgat tccgacagca tgaaccatct gaacaacacg ctgtatttcc gtctgatgga
    20581 agaggcgcgc atccagatcc tctacgccgc cggactgaag ctgccggacg accagggcgc
    20641 gatcctggcg cacgcgtcct gcgatttcct gcggccgctg acctatccgg ccaccgtgcg
    20701 ggtcacgcac gaggtcacgc gcatcggccg ttccagcctg gagttcgatc tcatcctcga
    20761 caagatcggc gacgcgacgg gttcctatgc ccgggggcgc aatgtcctgg tctggatgga
    20821 ctacctgaac aaccgctccg cgccctggcc ggccgaggtg ctgggcaggc tgggcggcct
    20881 gttcgttccc gcgggctagc gtcgtccggc agcgcgcttt gcgcgcgggc ggctgccgct
    20941 acaatcgttt cgtggaaggc atcgggccct tgcctcatcc ggtgccggca acaaatcacc
    21001 aaggagcagg agcaatgaac aaaatctatg cgaacgccac agaggcgctg gcaggcgtcg
    21061 tcaagaacgg ccagatgatc gccgtgggcg gcttcggcct gtgcggcatc cccgaggcgc
    21121 tgatcgccgc gctgcgcgat tcgggcgtca aggacctcac ctgcatcagc aacaacgccg
    21181 gcgtcgacgg tttcggcctg ggccagttgc tcaccacccg ccagatccgc aagatgatcg
    21241 cgtcctatgt gggcgagaac aaggaattcg agcgccagta cctggccggc gagctcgagc
    21301 tcgagttcac gccgcagggc accctggccg agaagctgcg cgccggcggc gcgggcattc
    21361 ccgccttctt caccaagacc ggcgtgggca ccatcgtggc cgacggcaag gaaatccgcg
    21421 agttcgacgg cgagcaatac gtcatggagc ggtcgctcgc gcccgacgtg gcgctggtga
    21481 aggccgacat cgccgaccgc agcggcaacc tggtgttccg caagacggcg cgcaacttca
    21541 accccaacgc ggccatggcg ggcaagatca ccatcgtcga agtcgagaag atcgtcgaaa
    21601 ccggcgcgct cgaccccgac cagatccacc tgcccggcat ctacgtgcat cggatcgtgc
    21661 tcaacacgac tcccgaaaag cgtatcgaac aacgcaccac gcgtaccgca taaggagaga
    21721 cacatggcat ggtcccgcga tgaaatggcg gcgcgcgccg cgcgcgaact gcaagacggc
    21781 tactacgtca acctgggcat cggcctgccc accctggtcg ccaaccacgt gcccgatggc
    21841 atggaggtct ggctccagtc cgagaacggc ctgctgggca tcggcccctt tcccacggaa
    21901 gacgaggtcg acgccgacct catcaacgcc ggcaagcaga cggtcaccac gctgcccggt
    21961 tcgtcgatct tctcgtcggc cgattccttc gcgatgatcc gcggcggcaa gatcaacctg
    22021 gccatcctgg gcgccatgca ggtgtccgag acgggcgacc tggccaactg gatgatcccc
    22081 ggcaagatga tcaagggcat gggcggcgcc atggacctgg tggccggcgt cggccgcgtc
    22141 gtggtgctga tggagcacgt ggccaagaag aaggacggca ccgaagacct caagctgctg
    22201 ccggcctgca cgctgcccct gaccggcgtg ggcgtggtcg acatcatcat caccgacctg
    22261 ggcgtgctgg aagtcactcc cggcggcctg aagctgctgg aagtcgcgcc cggcgtcacc
    22321 gtcgacgaaa tcaagtcgaa gacgtccgcc aagctcgacg tgtcggcggt caagtaggcc
    22381 ggcttgacgg atgggacagc cgcattacca catgcggtat cccatcctct tcatattcct
    22441 gcgtgacggc cacgaacccg tgcgcggcgt agaacccgcg caaatgggcc tgcgctccca
    22501 ggtacacccc cgcccgcggc cagcgtgagt gcgcctcggc cagcgcgcgc cgcatcagcg
    22561 cgtgtcccag gcccgtgccg cgccagccgg gcgccacgat cacgcggccg atgatcgctt
    22621 ctccctgctc gtcatgggcc gggtccagca gccggcagta cgccgccagc cggccgtcct
    22681 cccacgccat caggtgcgcc gtctcgccgc gcatgtcctg gccgtccatg tcctggaaaa
    22741 cacagcgctg ttccagcacg aatacggcgg tgcgcagctg cagcagcgca taaagctcgg
    22801 cggcgctcag gtcggcatgg tgcttgcaaa tccagtcggg catcgatgtt ccggcggtcg
    22861 aaagagtgga gcgcgcaggg taatgcaggc gcggcgggcc ggggcatgcc cggatttgag
    22921 gattcatttt ctcttatcgt gtttttgtat agcaattcga taccaggcaa ccatgagccc
    22981 atcatcgctg cgctctggcg ggccgaggcg actggctggc ccgcacggtt ctctgcgccg
    23041 caccccggcc cgatagaatg cggattgcag ccctcccgaa tccaggaacc ccatgcccgc
    23101 catccccacc cgtatcgccg ccctgaggca agccatgcgc cgcctcgggc tcgacgcctg
    23161 cgtcgtcccg tcctcggatc cgcatctttc cgaatacctg ccccagtatt ggcaaggccg
    23221 gcgctggctg tccggcttca cgggctcggt ggccaccctg gtggtgacgg ccgatttcgc
    23281 cggcctgtgg gtcgacagcc gctactgggt gcaggccggg agccagctgg ccggctcggg
    23341 catcgcgctg atgaagctgg ccgcgccagg cgtgcccggc catgtcgatt ggctggcggg
    23401 cacgctgccc gccggcgccc gcgtgggcgt ggacggacgg gtgctgggcc tggcgggcta
    23461 tcgcgcgctg acccaggcgc tggcgccggc cggcgtggcg ctggacatca tggccgaccc
    23521 cttgggcgag atctggacgg accgtccggc cttgcccggc gcgccggtcg ccgagcacct
    23581 gccgccttac gccaccgagt cgcgcgccca gcgcctggcg cgcgtgcgcg cagccatggc
    23641 ggcgcaccgg gccgacgtgc atctgctgtg cacgctggac gacatcgcct gggtgttcaa
    23701 cctgcgcggc gccgacgtgg actacaaccc ggtgttcctg gcccatgccc tgatcggcgc
    23761 cgaccatgcc accttgttcg tgggcgaagg caaggtgtcc gacgcgctgc gggcccggct
    23821 ggccgaggac ggcgtggcgg tcgcgccgta tgagcacgcc gccgaagccc tgggcacgat
    23881 cgaactggac cagacgctgc tgatcgaccc ggcccgcgtg acctgcggcg tgttccacct
    23941 gatggatccc gccgtggcgc gcatcgaggc catcaatccc tcgaccttct tcaagtccag
    24001 gaagagcgat gccgaactg
//
LOCUS       DASWPO010000247        12307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1577526, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000247 DASWPO010000000
VERSION     DASWPO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577526"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VGC69_12915"
     CDS             complement(<1..459)
                     /locus_tag="VGC69_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296226.1"
                     /translation="MNVTHLPARAIEPSPASTGAAMREQAYTEIKRRIIHCEFRPGEA
                     LNEAQIGAVLGLGRTPVHQALHRLEVEGLVSIMPRKGVLVSPLSLNDVLDTIEVRHTN
                     EVLCVTLAAQRAQEPDFQAMRDIVGPTPGLIAKRDIPALMTIDLKFHGAIS"
     gene            642..1289
                     /locus_tag="VGC69_12920"
     CDS             642..1289
                     /locus_tag="VGC69_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY0296227.1"
                     /translation="MLPFPPRPSFLPSASRLACGALNALLIREPWAGERLARHAGKTV
                     RFAWGRQRLSLAIASDGKVSEADEAIVPDVTLTLQSDKFKLANLLGGGMPDFAELTHI
                     SGDAGLAQVVGDLARDLRWDPEDDLARVVGDIPAARLVSGAQAVAAGARSAGSRLAAN
                     MAEYLAEESATLAGKPALAQQGLDLSRLQADADALERRIAAFEARIARVAGRGVR"
     gene            1286..2842
                     /gene="ubiB"
                     /locus_tag="VGC69_12925"
     CDS             1286..2842
                     /gene="ubiB"
                     /locus_tag="VGC69_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY0296228.1"
                     /translation="MMTMLRLIRIMFVAWRFGLDDLVLSSINHPLARGLRCMLRLGRH
                     PKAPRGQRLRQAFESLGPIFVKFGQVLSTRRDLIPADIADELSLLQDRVPPFPSAQAA
                     AIIEAALGAPPQALFAQFETDPVASASIAQVHFAVLHDGREVAVKVLRPGMLSVIEKD
                     MALLRVVARVIERLGADGRRLKPREVVAEFDKYLHDELDLVREASNCSQLRRNFGPET
                     GRSDMLIVPEVIWQYTASTVFTMQRMYGIPVSRIDRLRAAGIDIPALARGGVEIFFTQ
                     VFTDGFFHADMHPGNIYVSDNPSSLGRYIALDFGIVGSLSEFDKNYLAQNFLAFFRRD
                     YRRVAQLHIESGWVPRDTREEELEGAVRAVCEPYFDRPLAEISLGQVLLRLFQTSRRF
                     NVEIQPQLVLLQKTLLNVEGMGRQLDPDLDLWKTAKPHLENWMKKRMGLDGLRASLTR
                     ESAKWSQLLPALPRLLHDRLAHPDLNVELLAETRRLRRAREQGNRLLAALTAVLAVSV
                     AVALWALTRH"
     gene            3001..3879
                     /locus_tag="VGC69_12930"
     CDS             3001..3879
                     /locus_tag="VGC69_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0296229.1"
                     /translation="MLYPELFKTMEAVRWNMATDIPWGDFDGSKLSDEQACTIKMNAI
                     TEWAALPATEMFLRDNRGDSDFCAFMSVWFFEEQKHSLVLMEYLRRFRPELVPTEEEL
                     DKVRFEFDPAPELETLMLHFCGEIRLNHWYRCAAEWHTEPVIRAIYKTVAKDEARHAG
                     AYLQYMRRALHDRGETTSLDARYSFAKIGVLMASASRSAQAMHPTNLHVNKGLFPNDT
                     VQSRLPEPGWLERWLDTQIRFDGGWEGRVASRILHIMSKLLDSNFESVKDLNHYRKDV
                     MARMARVAADSKLIVA"
     gene            3955..4437
                     /gene="rfaE2"
                     /locus_tag="VGC69_12935"
     CDS             3955..4437
                     /gene="rfaE2"
                     /locus_tag="VGC69_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEY0296230.1"
                     /translation="MAIARFESKILPREAAVEAARHLPRPLVFTNGVFDILHRGHATY
                     LDQAACLGATLIVAVNTDESVRRLGKGPDRPLNNVEDRAALLAALACVAVVTSFHEDS
                     PEALIAELRPDIIVKGGDYDMEKLPETRLVRSWGGTAVAIPFEFQRSTTSLVKRIRQG
                     "
     gene            complement(4538..5242)
                     /locus_tag="VGC69_12940"
     CDS             complement(4538..5242)
                     /locus_tag="VGC69_12940"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296231.1"
                     /translation="MATNTVLSLPEMVYQHLRNAILNGTFTPGQVLRQEEVAVRLGVS
                     RSPLREALPRLEAEGIVVLNPRRGYSVAKIEPEEITEAFDLRVLLEVELARKSIARRT
                     DADIARVYAILNEMRAMSETAETADRTHWFELNANFHRSLLEPAKCPMHLRALRTTSG
                     TIEAYVRAEVRLTGDIKQAQVEHSMMGKAFAAGDIEQLVQIIRDHSSNTRVRLLEGLA
                     RTGHEPTPADFPEAEA"
     gene            5628..6989
                     /locus_tag="VGC69_12945"
     CDS             5628..6989
                     /locus_tag="VGC69_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0296232.1"
                     /translation="MNARKGYLLKGRVIDGSLNRPIEQGAVVTEGERIKWVGEAAALP
                     PAYAQEGYEVIALPGRTIMPGLIDGHTHISFGEARSEEENALYTPPEFRTLKAIFYAK
                     KILQAGVTSAFDAATTFGVAQAVRDAIDTGMFPGPRFTVSGKQITNHQGLEDSFPSNM
                     PFPEGQSAVLVKTRDDLIEAIRLQVKDEVDAIKVSGSNDNLITPDAQDTSAFTIEELR
                     IIVAETHRLGKLCSVHARSRDSSRDAAIAGFDNLFHASYIDEQGIEACLKNNSVITPT
                     LTLLVNLVEAGQSQAGASGSSAFQREIDAATVNLRRAYDAGVPLIAGSESGWSPVPYG
                     QWHAREMEIFVNVLGLTPLQAIHANTLAATRLLAPRYRDQVGLLAAGRLADILVVPGD
                     PTADIRILQRPSAFDYIFQGGAPIDRTPVPARRRKWYERTKTFLDGLYEFDEQAGAGR
                     LVR"
     gene            7069..8334
                     /locus_tag="VGC69_12950"
     CDS             7069..8334
                     /locus_tag="VGC69_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0296233.1"
                     /translation="MSNQAITWPTKDMSRIPYGAYHGEQIFEDEREKVFRGPTWNYLG
                     LECEIPDAGDFITTFVGDTSVLVSRHANGHVHAYVNRCKHRGAPLQRATFGNAKSHQC
                     IYHQWAYNLEGQLESVPFAKGMKGVGGLPSDFDKSSIHLDALKVEVYKGVIFATFSDH
                     AEPLLDYFGPEVIHELDRLFAKKVKVIGYQRQRIFGNWKLYNDNVRDPNHGGLLHMFH
                     VTFGLYRLGQVGGAKLDPKGRHNITYNQLGSDDTEAQKGYSDTNKVYQEGYSLRDMRM
                     LQYKNEHPDRVSLVIMSVFPSVVFQQIANSLCTRQIRPKGLDEVELYWTYFGYEDDTP
                     EMADHRLRQANLAGPGGYISMEDGEACEIVHDATLADKGSHSIVEIGGKGPVKEQKNL
                     ITEVPIRGFWGYYGELMGVDIDETAIARV"
     gene            8347..8820
                     /locus_tag="VGC69_12955"
     CDS             8347..8820
                     /locus_tag="VGC69_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY0296234.1"
                     /translation="MVTYEVYFAITDLFARCANALDDERLDDWLDCFGEDGSYYIRSR
                     ENWLRNLPLTLLQLDNKDMMHDRVVSLREANVTNIHRDQRVNSLPQITAGEAGTWNVV
                     SGYAMYTTNAEGRSTLFSVGSYVDRVRVADGKARFIERTVIVDTFAISNMLSTPI"
     gene            8849..9157
                     /locus_tag="VGC69_12960"
     CDS             8849..9157
                     /locus_tag="VGC69_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY0296235.1"
                     /translation="MSYLYAADLSSLAEDDAHPVDLQGLQLALYLVEGRVYATSNICT
                     HQKVLLSDGFMEDGCIECPLHAGRFDVRTGAALCEPATQPLKVFPVRIEDNRVMVDVG
                     "
     gene            9305..9724
                     /locus_tag="VGC69_12965"
     CDS             9305..9724
                     /locus_tag="VGC69_12965"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0296236.1"
                     /translation="MTTAVQYVNPEGACPAQGLYSHVGVPAAGAQLLFVAGQLSVAAD
                     GSVAGKHDFDAQFHQIFSNLGVVLKGLGCDFNDVVKFTTYLPHSQDIEHFMRLRAELF
                     PKIFKGPLFPPNTLLVVDRLVKEDFLLEVEAIVQAKA"
     gene            9755..10573
                     /locus_tag="VGC69_12970"
     CDS             9755..10573
                     /locus_tag="VGC69_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0296237.1"
                     /translation="MSLKEFSLTVDGLRAQCWEGGSGTPLLLLHGSGPGAASQGTWRL
                     VLEPLMARYRVLATDLIGFGMSDRKAEGPFFDIDLWMKQAHALLDYAGGEKAYVIGHS
                     LSGYLAMRLASEDSRVAKLVTTGTMGANFACNAETEKCWTFPQTREQLRSTLESLMFD
                     RSGITDELLDYRMGILHDGKYGPYFSSMFAGDKQRCIKAASLSPEQLQRIRCPVLMIH
                     GREDIMFPAEGTTMEISKHLPQADILLLSQCRHLPAAEHPGKVAAAVTDFFGPV"
     gene            10605..11696
                     /locus_tag="VGC69_12975"
     CDS             10605..11696
                     /locus_tag="VGC69_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0296238.1"
                     /translation="MFRTLIAAVLAAIAACLLSLPIAQTADAFTLKGKPKIAMIYFGP
                     KNDGGWTQSFDEARVKMEAELGQKIQFVESVPEDASAIKPAVERFIQRGANIIIGGAF
                     GYSDTFKELAAKYPDVAFLNGAGTTNGPNLLGFYGRTYESQYLCGMAAAAVSKTGKLG
                     FVAANPFGMINWAINAYELGARKINPAATVNVVFTGVWNDPVKERAAATALIDQGADV
                     IGQHVDSPTPQIVAQERGVYATGHHRDLSEFAPKATVCSSMWVWDKFLTSEVRHIEAG
                     DWQPSPYGAFVKMKDGGTDISPLGAAVPKDKAALIMAERDAIIGGKQIYAGPMSDRDG
                     KERIPAGQSLSDAELWKMDWYVPGVITQK"
     gene            11721..12251
                     /locus_tag="VGC69_12980"
     CDS             11721..12251
                     /locus_tag="VGC69_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0296239.1"
                     /translation="MNALELSGICKSFDGFQALDRADFAVRWGEVHALLGENGAGKSS
                     LMNIAAGLYAPEEGRILVDDNLVRLSGPREAARHRIGMVHQHFKLVRAFTAAENILLG
                     HPLASELGSHRSRLRRLEGLIRAKAAELGFDLDPGRRVDRMSIAEQQRVEILKVLMAG
                     ARILILDEPTAVLTDQ"
BASE COUNT         2141 a         4081 c         4005 g         2080 t
ORIGIN      
        1 cgagatggcg ccgtggaact tgaggtctat cgtcatcagg gcggggatgt cgcgcttggc
       61 gatcaggccg ggcgtcggcc cgacgatgtc gcgcatggcc tggaaatcgg gctcctgggc
      121 gcgctgggcg gccagcgtca cgcacagcac ttcgttggtg tggcgcactt cgatggtgtc
      181 gagcacgtcg ttgagcgaca ggggcgagac cagcacgccc ttgcgcggca tgatggacac
      241 cagcccttcg acctcgagcc ggtgcagggc ctggtgcacc ggcgtgcggc ccaggcccag
      301 cacggcgccg atctgggctt cgttcagcgc ctcgccgggg cggaactcgc aatggatgat
      361 gcggcgcttg atctcggtgt aggcctgctc gcgcatcgcg gcgccggtgc tggcggggga
      421 cggctcgatg gcgcgggcgg gaaggtgggt gacgttcatg taggggtatc cctgataatc
      481 atctaataat agagtgatat atcactgaag aaaatcatgg aaaacccggc caggaaacct
      541 ggcgcgcccg ggaagaaggg ccgggctggc ggtgcgaacc cggtaaactc tggcttgcgt
      601 tacccggccg agatgcgccg cggccgccat tcctggattc catgctgcct ttcccgcctc
      661 gaccgtcttt tctgccttcg gcgtcccgcc tcgcctgcgg cgcgctcaat gcgctgctga
      721 tccgcgagcc ctgggcaggc gaacgcctgg cccgccatgc gggcaagacg gtgcgcttcg
      781 cctgggggcg gcagcgcctg tccctggcca tcgccagcga cggcaaggtg tcggaggccg
      841 acgaggccat cgtgcccgac gtgacgctga ccctgcagtc cgacaagttc aagctcgcga
      901 acctgctggg cggcggcatg cccgactttg ccgagctgac gcacatctcg ggcgatgccg
      961 ggctggccca ggtggtgggc gacctggcgc gcgacctgcg ctgggatccg gaagacgacc
     1021 tggcccgcgt ggtgggcgac atcccggccg cgcgcctggt gtcgggcgcg caggcggtgg
     1081 ccgccggcgc gcgcagcgcg ggcagccgcc tggcggcgaa catggccgag tacctggccg
     1141 aagaaagcgc caccctggcg ggcaagccgg ccctggcgca gcagggcctg gacctgtccc
     1201 gcctgcaggc cgatgccgac gcgctggagc ggcgcatcgc cgcgttcgag gcccgcatcg
     1261 cgcgcgtggc gggccggggc gtccgatgat gaccatgctg cgcctgatcc gcatcatgtt
     1321 cgtggcctgg cgcttcgggc tcgacgacct ggtcctgtcc agcatcaacc atccactggc
     1381 gcgcggcctg cggtgcatgc tgcggctggg ccggcatccg aaggcgccgc gcggccagcg
     1441 cctgcgccag gcgttcgagt cgctgggccc catcttcgtg aagttcggcc aggtgctgtc
     1501 tacccgccgc gacctgatcc cggccgacat cgccgacgag ctgtccctgc tgcaggaccg
     1561 cgtgccgccg ttcccgtcgg cgcaggcggc cgccatcatc gaggcggcgc tgggcgcgcc
     1621 gccgcaagcg ttgttcgcgc agttcgaaac cgaccccgtc gcctcggcct cgatcgccca
     1681 ggtgcatttc gcggtgctgc acgacggccg cgaagtggcc gtgaaggtgc tgcgccccgg
     1741 catgctcagc gtcatcgaga aggacatggc cctgctgcgc gtcgtggcgc gcgtcatcga
     1801 gcgcctgggc gccgacggcc ggcggctcaa gccgcgcgaa gtggtggccg agttcgacaa
     1861 atacctgcac gacgagctcg acctggtgcg cgaagcctcc aattgcagcc agctgcgccg
     1921 caatttcggc cccgagacgg gccgctccga catgctcatc gtgcccgagg tgatctggca
     1981 atacaccgcc agcacggtgt tcaccatgca gcgcatgtac ggcatcccgg tgagccgcat
     2041 cgaccggctg cgcgcagccg gcatcgacat ccccgcgctg gcgcgcggcg gggtcgagat
     2101 cttcttcacc caggtgttca ccgacggctt ttttcacgcc gacatgcacc ccggcaatat
     2161 ctatgtgtcc gacaacccgt cgtcgctggg gcgctacatc gcgctggatt tcggcatcgt
     2221 cggctccctg tcggagttcg acaagaacta cctggcgcag aacttcctgg ccttcttccg
     2281 ccgcgactac cgccgcgtgg cgcagttgca catcgagtcg ggctgggtgc cgcgcgacac
     2341 gcgcgaggaa gagctcgaag gcgccgtgcg cgcggtatgc gagccctatt tcgaccggcc
     2401 gctggccgag atctcgctcg ggcaggtgct gctgcgcctg ttccagacct cgcgccgctt
     2461 caatgtcgag atccagccgc agctggtgct gctgcagaaa accctgctca acgtcgaggg
     2521 catgggccgc cagctcgacc ccgacctgga tctctggaag accgccaagc cgcacctgga
     2581 aaactggatg aagaagcgca tgggcctgga cggcctgcgc gcgagcctga cccgggaatc
     2641 ggccaaatgg tcgcaacttc tgccggccct gccgcgcctg ctgcacgacc gcctcgccca
     2701 tcccgacctg aacgtcgaac tgctcgccga aacgcgccgc ctgcgccggg cgcgcgagca
     2761 gggcaaccgc ctgctcgcgg cgctgacggc cgtgctggcc gtttccgtgg cggtggcttt
     2821 atgggcgctg acccggcact gatacatata taagcgccgg cgcgagcgcc gcacctgtta
     2881 ttgtatgacc gggactgggc tggtcatatt tccgacacga aactgtcatc acagtttctt
     2941 cctgggcagc gataaataca tctgtacaag actgcccgcg aactttacgc gtaggacgac
     3001 atgctttatc ccgaactttt caagaccatg gaagccgtgc gctggaacat ggcgaccgac
     3061 atcccgtggg gtgacttcga cggcagcaag ctgtccgacg aacaggcctg cacgatcaag
     3121 atgaacgcca tcaccgagtg ggcggcgctg ccggccaccg agatgttcct gcgcgacaat
     3181 cgtggcgaca gcgatttctg cgcgttcatg tcggtatggt tcttcgagga gcagaagcat
     3241 tcgctggtgc tgatggagta cctgcgccgc ttccgtcccg aactggtgcc caccgaagaa
     3301 gagctggaca aggtgcgctt cgagttcgat cccgcgcccg agctcgaaac cctgatgctg
     3361 cacttctgcg gcgaaatccg cctgaaccac tggtaccgct gcgccgccga atggcatacc
     3421 gagcccgtca tccgcgccat ctacaagacg gtggcgaaag acgaggcgcg ccatgccggc
     3481 gcctatctgc aatacatgcg ccgcgccctg cacgaccgcg gagaaacgac cagcctcgat
     3541 gcgcgttatt cgttcgccaa gatcggcgtg ctgatggcct cggccagccg ttcggcgcag
     3601 gccatgcacc ccaccaatct gcacgtcaac aaggggctct tccccaacga cacggtgcag
     3661 tcgcggctgc ccgagccggg ctggctggaa cgctggctcg acacgcagat ccgcttcgac
     3721 ggcggttggg aaggccgcgt ggccagccgc atcctgcaca tcatgtccaa gctgctcgac
     3781 agcaatttcg agtcggtcaa ggacctcaac cactaccgca aggacgtgat ggcgcgcatg
     3841 gcgcgggtgg cggcggacag caagctgatc gtggcttgac cgccaggcgg cctgtccgcc
     3901 ggctcggcgg ctcccgtccc tgcgcacccc ggccgcggct acaatcgccg cactatggcc
     3961 atcgcccgat tcgaatccaa gatcctgccg cgtgaggccg ccgtcgaggc ggccaggcac
     4021 ttgccgcgtc ccctggtgtt caccaacggg gtgttcgaca tcctgcaccg cggccatgcc
     4081 acctatctcg atcaggcggc ctgcctgggc gccaccctga tcgtggcggt caacaccgac
     4141 gagtcggtac gccgcctggg caagggcccg gaccggccgc tcaataacgt ggaagaccgc
     4201 gccgcgctgc tggcggcgct ggcctgcgtg gcggtggtga cgtcctttca cgaagactcg
     4261 cccgaggccc tgatcgccga gctgcgcccc gacatcatcg tcaagggcgg cgactacgat
     4321 atggaaaaac tgcccgagac ccggctggtg cgcagctggg gcggcacggc cgtggcgatt
     4381 cccttcgagt tccagcgctc gacgacttcg ctggtcaagc ggatacggca gggctaggcc
     4441 gggacggatc gggccgtccc gaggccgcct taacacggct cgtttttata cagtatgcaa
     4501 agccgggatg ttcggggcgg caagcgcggc ggcctgccta ggcttcggct tcggggaagt
     4561 cggcgggggt cggttcgtgc ccggtgcggg ccaggccttc gagcagccgg acgcgggtat
     4621 tgctcgagtg gtcgcggatg atctgcacga gctgttcgat atccccggca gcgaaggcct
     4681 tgcccatcat gctgtgctcg acctgggcct gcttgatgtc gccggtgagc cggacttcgg
     4741 cgcgcacgta ggcttcgatc gtgccgctgg tggtgcgcag cgcgcgcagg tgcatcgggc
     4801 acttcgccgg ttccagcagc gaccggtgga agttggcgtt caattcgaac cagtgcgtgc
     4861 ggtccgcggt ctcggcggtt tccgacatgg cgcgcatttc gttcaggatg gcatagactc
     4921 gggcgatatc ggcgtcggtg cggcgggcga tcgatttcct ggcgagctcc acttcgagga
     4981 ggacgcgcag gtcgaaggct tcggtgattt cttcgggctc gatcttggcg accgaatagc
     5041 cgcgccgggg attgagcacc acgatgccct cggcctcgag gcggggcagc gcctcgcgca
     5101 gcgggctgcg gctgacgccc aggcgcacgg caacctcttc ctggcgtaga acctggcccg
     5161 gcgtgaacgt gccgttgagg atcgcgttgc gcaggtgctg gtagaccatt tccggcagtg
     5221 ataggactgt atttgtcgcc atggcagagg aatcggacag cggggaggga ttggaatttt
     5281 tattttctcg atattgcata aagtactctg ccacaaccat gatttgtatg gacggatgaa
     5341 atgatagccg atccaaatcc gtttgcaggc ggctaatatg cctccaggca tcaagatttt
     5401 gcgatcatgt gcctgattta catgaattta taccgcattt cgcatggatt gcaggcatgc
     5461 cggaacaggc ttgggcggcg aggagggccc cagcgaccca tggttttccc gcatgcatgg
     5521 atcccacccc ttgattttat aattgtataa aatctactta aacaaaacac accaatgttc
     5581 caagccgggt tggcctgtgc ggcgaacggg gcatgcggag aaagccaatg aatgcacgca
     5641 aggggtacct gctcaagggg cgcgtcatcg atgggagctt gaacaggccc atcgaacagg
     5701 gcgccgtggt caccgagggc gagcggatca agtgggtggg cgaggcggcg gccctgccgc
     5761 cggcctacgc gcaggaaggc tacgaggtca tcgccttgcc gggccgcacg atcatgccgg
     5821 gcctgatcga cgggcatacg cacatctcgt tcggcgaagc ccgttccgag gaagaaaacg
     5881 cgctctatac gccgccggag ttccgcacgc tcaaggcgat cttctacgcc aagaagatcc
     5941 tgcaggccgg cgtgaccagc gcgttcgatg cggcgaccac gtttggcgtc gcgcaggccg
     6001 tgcgcgacgc catcgatacc ggcatgttcc cggggccgcg tttcacggtt tccggcaagc
     6061 agatcaccaa ccaccagggc ctcgaggatt ccttccccag caatatgcct ttccccgaag
     6121 ggcaaagcgc cgtcctggtc aagacgcgcg acgatctgat cgaggccatc cgcctgcagg
     6181 tcaaggacga ggtcgacgcc atcaaggtct ccggctccaa cgacaacctc atcacccccg
     6241 acgcgcagga cacctcggcc ttcacgatcg aggagctgcg catcatcgtc gcggaaaccc
     6301 atcgcctggg caagctgtgc agcgtgcatg cgcgctcgcg cgattcgtcg cgggatgccg
     6361 cgatcgccgg tttcgacaac ctgttccacg cctcctacat cgacgagcaa ggcatcgagg
     6421 cctgcctcaa gaacaactcc gtcatcacgc ccacgctgac gctgctggtc aacctggtcg
     6481 aggcgggcca gtcgcaggcg ggcgcatccg gctcgtcggc cttccagcgc gagatcgacg
     6541 ccgcgacggt gaacctgcgg cgcgcctacg acgccggggt tcccttgatc gccggcagcg
     6601 aaagcggctg gtctccggtg ccttatggcc agtggcacgc ccgcgaaatg gagatcttcg
     6661 tcaatgtcct ggggctgacg ccgctgcagg ccatccacgc caataccctg gccgccacgc
     6721 gcctgctggc gccgcgctat cgcgaccagg tcgggctgct cgcggccggg cgcctggccg
     6781 acatcctggt cgtgcccggc gatcccacgg ccgacatccg catcctgcag cggccgtcgg
     6841 cattcgacta catcttccag ggcggggccc ccatcgaccg cacgccggtg ccggcccgcc
     6901 gccgcaagtg gtacgaacgc accaagacat tcctcgacgg gctctacgag ttcgacgagc
     6961 aggccggggc cggccgcctg gtccggtagc cgtccgggat acggccgggc ccggcgcagg
     7021 ccgggccccg cttgcgcaaa cgcatgattt cctgcaagga gcacgttgat gtccaaccaa
     7081 gctatcactt ggccgaccaa ggatatgtcg cgcatccctt atggcgcata ccacggggaa
     7141 cagatcttcg aagacgagcg cgagaaagtc ttccgcggcc ccacctggaa ctatctcggc
     7201 ctggaatgcg agatccccga cgccggcgac ttcatcacga ctttcgtggg cgacacctcg
     7261 gtcctggtca gccgccatgc gaacggccat gtccatgcct acgtcaaccg gtgcaagcac
     7321 cgcggcgcgc cgctgcagcg ggcgaccttc ggcaacgcca agagccacca gtgcatctac
     7381 caccagtggg cctacaacct ggaaggccag ctcgagagcg ttcccttcgc caagggcatg
     7441 aagggcgtgg gcggcctgcc gagcgacttc gacaaatcga gcatccatct cgatgcgctc
     7501 aaggtcgagg tgtacaaggg cgtgatcttc gcgaccttca gcgaccatgc cgagccgctg
     7561 ctcgactact tcggtccgga agtcatccat gagctcgacc gcctgttcgc caagaaggtc
     7621 aaggtcatcg gctaccagcg ccagcgcatc ttcggcaact ggaagctcta caacgacaac
     7681 gtgcgcgacc ccaaccacgg cggcctgctg cacatgttcc acgtcacctt cggcctgtac
     7741 cgcctgggcc aggtcggcgg cgcgaagctg gaccccaagg ggcgccacaa catcacctac
     7801 aaccagctcg gcagcgacga taccgaggcc cagaagggct actccgatac caacaaggtc
     7861 taccaggaag gctacagcct gcgcgacatg cggatgctcc agtacaagaa cgagcacccc
     7921 gaccgcgtca gcctggtgat catgtcggtg tttcccagcg tcgtgttcca gcagatcgcc
     7981 aattcgctct gcacgcgcca gatccgcccc aagggcctgg acgaggtcga gctgtactgg
     8041 acctatttcg gctacgagga cgacacgccc gagatggcgg accaccgcct gcgccaggcc
     8101 aacctggccg gtcccggcgg ctacatctcg atggaagacg gcgaggcctg cgagatcgtc
     8161 cacgacgcga ccctggcgga caagggcagc cactccatcg tcgagatcgg cggcaagggg
     8221 ccggtcaagg aacagaaaaa cctgatcacc gaagtgccca tccgcggatt ctggggctac
     8281 tacggcgagc tcatgggcgt ggacatcgac gaaaccgcaa tcgccagggt ctgaagaaac
     8341 atcatcatgg ttacctacga agtctatttc gcgatcaccg atctgtttgc gcgctgcgcc
     8401 aacgcgctgg acgacgagcg gctggacgat tggctggatt gtttcggcga agacggcagc
     8461 tactacatcc gttcccgcga gaactggctg cgcaatctgc cgctgacgct gctgcagctg
     8521 gacaacaagg acatgatgca cgatcgcgtg gtgtccctgc gcgaggccaa cgtcaccaac
     8581 atccaccgcg accagcgggt gaacagcctg ccgcagatca cggccggcga ggcggggacg
     8641 tggaacgtgg tgtcgggcta cgccatgtac acgaccaatg ccgaaggccg cagcacgctg
     8701 ttctcggtgg gcagctatgt ggaccgcgtc cgcgtcgcgg atggcaaggc gcgcttcatc
     8761 gagcggaccg tcatcgtcga taccttcgcg atttccaaca tgctttccac acccatctga
     8821 gcccgaaccg gaccggccat cgcctttcat gagctacctc tacgccgccg atctgtcctc
     8881 gcttgccgag gacgacgccc atcccgtcga tctccagggg ctgcagctgg ccttgtacct
     8941 ggtcgaaggc cgggtctacg ccacgagcaa tatctgcacg caccagaagg tcctgctgtc
     9001 cgacggcttc atggaagacg gatgcatcga gtgcccgctg cacgcggggc ggttcgacgt
     9061 caggaccggc gccgccctgt gcgagccggc cacgcagccg ctgaaggtct ttcccgtgcg
     9121 catcgaagac aaccgggtga tggtcgatgt cggctgagcg ccgcgcctcg gccgccgcgg
     9181 ccggttcagg aagaagccgg cctgtccggc ctgtgcttta cgccctatcg ctgtgcctga
     9241 tccccgggag caggcacggc tgtttttgct aaacaacatc acatttcatc cacggagttc
     9301 gctcatgacc actgctgttc aatatgtcaa tccggaaggc gcctgcccgg cccagggctt
     9361 gtattcgcac gtcggcgtgc ccgccgcggg cgcccagctg ctgttcgtgg cgggacagct
     9421 gtcggtggcc gcggatggca gcgtcgccgg caagcacgac ttcgacgccc agttccacca
     9481 gatcttcagc aatctgggcg tggtcctcaa gggactcggc tgcgacttca acgacgtggt
     9541 caagttcacc acctacctgc cccattcgca ggacatcgag cacttcatgc gcctgcgcgc
     9601 cgagctgttc cccaagatct tcaagggccc gctcttcccg cccaacacgc tgctggtggt
     9661 cgatcgcctg gtcaaggaag acttcctgct ggaagtcgag gccatcgtcc aggccaaggc
     9721 ctgattttcc acgtccagat cgtctggagg aagtatgtcg ctcaaggaat tttcgctcac
     9781 cgtggacggc ctgcgggccc agtgctggga agggggcagc ggcacgccgc tgctgctgct
     9841 gcacggctcg ggccccggcg ccgcgtcgca gggcacctgg aggctcgtgc tcgagccgct
     9901 gatggcgcgc tatcgcgttc tggccaccga cctgatcggc ttcggcatgt cggaccgcaa
     9961 ggccgagggc cccttcttcg acatcgacct ctggatgaag caggctcatg cgctgctgga
    10021 ttacgccggc ggtgagaaag cctatgtcat cgggcattcg ctctcgggct atctggcgat
    10081 gcgcctggcg tccgaggaca gccgggtggc caagctggtg accaccggca ccatgggggc
    10141 gaacttcgcc tgcaatgccg agaccgagaa gtgctggact tttccgcaga cgcgcgagca
    10201 attgcgctcg acgctggaga gcctgatgtt cgacaggtcg ggcatcaccg acgagctgct
    10261 cgactatcgc atgggcatcc tgcacgacgg caagtacggg ccgtacttca gcagcatgtt
    10321 cgcgggcgac aagcagcgct gcatcaaggc cgccagcctg tcgccggaac agttgcagcg
    10381 gatccggtgc ccggtgctca tgatccacgg cagggaagac atcatgttcc cggccgaggg
    10441 caccacgatg gaaatatcga agcacctgcc gcaggccgac atcctgctgc tgtcgcaatg
    10501 ccggcatctg cccgcggccg agcatcccgg caaggtggcg gccgccgtga ccgatttctt
    10561 cggaccggtt tgatccctgt ttgatctctt ataggaagac acccatgttc cgcaccctaa
    10621 tcgctgcagt cctggctgcg atcgcggcct gtctgctgtc cttgcccatc gcgcagacgg
    10681 ccgatgcctt cacgctcaag ggcaagccga aaatcgcgat gatctatttc ggccccaaga
    10741 acgacggcgg ctggacgcag tcgttcgacg aggcgcgggt caagatggag gccgagctcg
    10801 gccagaagat ccagttcgtc gagagcgtcc ccgaagacgc ctcggcgatc aagccggccg
    10861 tcgagcgctt catccagcgc ggcgccaata tcatcatcgg cggggctttc ggctattcgg
    10921 acacgttcaa ggagctggcg gcgaaatatc ccgacgtggc cttcctcaac ggcgccggga
    10981 ccaccaacgg gcccaacctg ctgggctttt atggccgcac ctatgagagc cagtacctgt
    11041 gcggcatggc ggccgccgcc gtgtccaaga cgggcaagct gggcttcgtc gcggccaacc
    11101 ccttcggcat gatcaactgg gcgatcaatg cctatgagct gggcgcgcgc aagatcaacc
    11161 cggcggcaac cgtgaacgtg gtcttcaccg gcgtatggaa cgacccggtc aaggagcgcg
    11221 ccgcggcaac ggccctgatc gaccaggggg ccgacgtgat cggccagcac gtcgattcgc
    11281 ccacgccgca gatcgtcgcg caggagcgcg gcgtgtacgc caccggccac caccgcgacc
    11341 tgagcgagtt tgcgcccaag gcgaccgtgt gctcgtcgat gtgggtctgg gacaaattcc
    11401 tgacctcgga ggtccggcac atcgaggcgg gcgactggca gccttcgccc tatggcgcct
    11461 tcgtcaagat gaaggacggc ggcaccgaca tctcgcccct gggcgcggcc gtgcccaagg
    11521 acaaggccgc gctgatcatg gccgagcgcg acgcgatcat cggcggcaag cagatctacg
    11581 cgggcccgat gtccgaccgg gacggcaagg agcgcatccc ggcgggccag tccctgtcgg
    11641 acgccgagct ctggaagatg gactggtacg tgccaggcgt gatcacgcag aagtaaggcg
    11701 gactcgtcgt gggcatgggc atgaacgcgc tggagctgtc gggcatctgc aagtccttcg
    11761 acggtttcca ggcgctggac cgcgccgatt tcgcagtgcg ctggggcgag gtgcacgcct
    11821 tgcttggcga gaacggcgcc ggcaagtcgt cgctgatgaa catcgccgcc gggctgtatg
    11881 cgccggaaga agggcgcatc ctggtggacg acaacctggt gcgcctgagc gggccgcgcg
    11941 aggccgcccg gcatcgcatc ggcatggtcc accagcattt caagctggtg cgggccttca
    12001 cggcggccga gaacatcctg ctcggccatc cgctggcctc ggagctgggc agccatcgca
    12061 gccggctacg ccggctggag ggcctgatcc gcgccaaggc cgccgagctg ggtttcgatc
    12121 tcgaccccgg ccgccgggtc gaccggatgt cgatcgccga gcagcagcgc gtcgagatcc
    12181 tcaaggtgct gatggccggc gcgcgcatcc tgatcctgga cgagccgacc gccgtgctga
    12241 ccgaccagta ggccgcgcgc ctgctggaca tggtgcgcgg cttggcgcgc ggcggcgcgg
    12301 cggtggc
//
LOCUS       DASWPO010000248         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2827748, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000248 DASWPO010000000
VERSION     DASWPO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827748"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VGC69_12985"
     CDS             <1..139
                     /locus_tag="VGC69_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296240.1"
                     /translation="LGFVEVLSVAYIGSTMRDAVAFGLLFLILLVRPQGVFGKVVQRK
                     A"
     gene            144..1094
                     /locus_tag="VGC69_12990"
     CDS             144..1094
                     /locus_tag="VGC69_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296241.1"
                     /translation="MDFQNFWDVYSNLVLTLGTNVLLALSIWLTLNCGMLAVANAAFL
                     GIGAYTSSLLTMNYDTPFYVSLAGGMILPALIATLIGLPLIRLSGVYLAIATLAFGEV
                     VRVVILNTDALTGGAMGLNGIPQLTQGWHVLLAVVVVLFLLWRVRASKVGRAFDAIRG
                     DETAAGLMGINVRANKMLAFVAGAAIAGLAGGLNAHLNFFIGPNEYGFDRGVEILTMA
                     ILGGVGGLAGPVLGALILTLLPTTLGFLGDYKLMVNGLILVLIVLFLPQGIWDPVRFK
                     RLLRKSPHAAPVGLAAPQGGTSASGRPGRGRQTNEEDHHA"
     gene            1087..1860
                     /locus_tag="VGC69_12995"
     CDS             1087..1860
                     /locus_tag="VGC69_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296242.1"
                     /translation="MLELSSLSKRFGGLHVLHDVNLTVPQGAIFGLIGPNGAGKTTVF
                     NLITGLLAPTAGSIAFDGRSLLGRKPHQITRLGIARTFQNIRLFKEMTLLENVVVGAY
                     RHMDYGFPGLLLGLPKYREHEARARARALELLSWMKLDHKAHDLADNLSYGEQRRLEL
                     ARALATEPRLLLLDEPVAGMNTGERAELMTEILAIRDRGYTILMIEHDMRFVMGLCET
                     IAVLNFGKIIACGGPDAIRNNEQVIEAYLGRDDDEEAAA"
     gene            1857..2573
                     /locus_tag="VGC69_13000"
     CDS             1857..2573
                     /locus_tag="VGC69_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296243.1"
                     /translation="MTGKPMLEVRGLEINYGHIEAVRGIDFTLNSNEITALVGANGAG
                     KSTTLLALSGLLPKTRGQVLFEGEDITRLAPHQIVARGIVQVPEGRAILTTMTVQENL
                     ELGAYRRGLKAAASELDYVLHLFPRLQERLAGIAGNLSGGEQQMLAIGRALMAKPRLL
                     FLDEPSMGLAPIVVQEIFRSLRAINGTGLTLFLVEQNVRQALKIAQHGYVLENGAMAL
                     SGTGGELLGHPRVLQAYLGS"
     gene            2596..3699
                     /locus_tag="VGC69_13005"
     CDS             2596..3699
                     /locus_tag="VGC69_13005"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEY0296244.1"
                     /translation="MIKRLAHTLSAWLQSLARPIQWLILLALSSLFLFALERAGLPAG
                     ILLGPLVAGAILSILGVRLQLPRRLGTLIPGVVGCMIVKLMPSSGFGQIADHLPVFLF
                     GTFAVIIASGFLGWVMTRMRILPGTTALWGLSPGAASAMVLMAEAYGADTALVALMQY
                     LRVLLVTSITASVATLAGASHMQHAAISMGSLSDVAWLPLAETLVLALGGAALGRALR
                     VPGGTMLLPMVLAFIFGHSGLVELELPRGLLLAVYALLGWRIGFRFTAPLLAHAARAM
                     PRIILATLILMALCGALAGMLVLTTGIDPLSAYLATSPGGVDSVAIIAASTHVDVTFV
                     MGMQTVRFLGVLLLGPIMVRLLARSTQRDARPT"
BASE COUNT          555 a         1415 c         1150 g          630 t
ORIGIN      
        1 cctgggcttc gtcgaggtgc tgtcggtggc gtacatcggc tcgaccatgc gcgacgccgt
       61 ggctttcggc ctgctatttc tcatcctgct cgtccgcccg caaggcgtgt tcggcaaagt
      121 ggtgcaacgc aaggcttaaa acaatggatt tccaaaattt ctgggacgtc tacagcaacc
      181 tggtgctcac gctgggcacc aacgtgctgc tggcgctctc catctggctc acgctgaact
      241 gcggcatgct ggccgtggcc aatgccgcct tcctgggcat cggcgcctat acctcctcgc
      301 tgctcacgat gaactacgac acgccgttct acgtgtcgct ggccggaggc atgatcctgc
      361 ccgccctcat cgccaccctc atcggcctgc ccctcatccg cctgtcgggg gtgtacctcg
      421 ccatcgcgac gctggccttc ggcgaagtgg tccgcgtggt catcctgaac accgacgcct
      481 tgacgggcgg cgcgatgggc ttgaacggca tcccacaact cacgcaaggc tggcacgtgc
      541 tgctggccgt cgtggtcgtg ctgttcctcc tgtggcgcgt gcgcgcctcc aaggtgggcc
      601 gcgccttcga cgccatccgc ggcgacgaga cggcggccgg cctgatgggc atcaacgtgc
      661 gcgccaacaa gatgctggcc ttcgtggcgg gcgccgccat cgccgggctg gcgggcggcc
      721 tcaatgccca cctcaacttc ttcatcggcc ccaacgaata cggcttcgac cgcggcgtgg
      781 aaatcctgac catggccatc ctgggcggcg tcggcgggct cgccggcccc gtcctgggcg
      841 ccctcatcct caccctgctg cccacgacgc tgggcttcct gggcgactac aaactgatgg
      901 tcaacggcct catcctcgtg ctcatcgtcc tgttcctgcc gcaaggcatc tgggaccccg
      961 tccgcttcaa gcgcctgtta aggaaaagcc cccacgccgc gcccgtcggg cttgctgccc
     1021 cccaaggcgg cacgtccgcc tcggggcggc ccggccgcgg gcggcaaact aacgaggagg
     1081 accatcatgc ttgagctctc ctccctctcc aagcgcttcg gcggcctgca cgtcctgcac
     1141 gacgtcaacc tcacggtgcc gcaaggcgcc atcttcggcc tgatcggccc caacggcgcc
     1201 ggcaagacca ccgtgttcaa cctgatcacc ggcctgctgg cgccgaccgc cggcagcata
     1261 gccttcgacg ggcgaagcct gctgggccgc aagccgcacc agatcacccg cctgggcatc
     1321 gcccgcacgt tccagaacat acgcctgttc aaggaaatga ccctgctcga gaacgtcgtg
     1381 gtgggcgcct accgccacat ggactacggc tttcccggcc tgctgctggg cctgcccaag
     1441 taccgcgaac acgaggcccg cgcccgcgcc cgcgcgctcg agctgctgtc atggatgaaa
     1501 ctcgaccaca aggcgcacga cctggccgac aatctgtcct acggcgaaca gcgccgcctc
     1561 gaactggccc gggcgctcgc caccgaaccc cgcctgctgc tgctcgacga acccgtggcc
     1621 ggcatgaaca ccggcgaacg cgccgagctc atgaccgaga tcctggccat ccgcgaccgc
     1681 ggctacacca tactcatgat cgaacacgac atgcgctttg tgatgggcct gtgcgaaacc
     1741 atcgccgtgc tcaacttcgg caagatcatc gcctgcggcg gccccgatgc catccgcaac
     1801 aacgagcagg tgatcgaggc ctacctgggc cgcgacgacg acgaggaggc ggcggcatga
     1861 cgggcaaacc catgctcgag gtacgcggcc tggagatcaa ctacggccac atcgaagccg
     1921 tgcgcggcat cgacttcacc ctcaatagca acgagatcac cgccctggtc ggcgccaacg
     1981 gcgccggcaa gtcgaccacc ctgctggcgc tctccggcct gctgcccaag acccgcggcc
     2041 aggtgctctt cgaaggcgaa gacatcaccc ggctggcgcc gcaccagatc gtcgcgcgcg
     2101 gcatcgtgca ggtgccggaa ggccgggcca tcctcaccac catgacggtg caggagaacc
     2161 tggaactcgg cgcctatcgc cgcggcctga aggccgccgc gagcgagctc gactacgtgc
     2221 tgcatctctt cccgcgcctg caggaacgcc tcgccggcat cgccggcaac ctgtcgggcg
     2281 gcgaacagca gatgctggcc atcggccgcg cgctcatggc caagccgcgc ctgctgttcc
     2341 tggacgagcc ttccatgggc ctggcgccca tcgtcgtgca ggaaatcttc cgctcgctgc
     2401 gcgccatcaa cggcaccggg ctcaccctgt tcctggtcga gcagaacgtg cgccaggcgc
     2461 tcaagatcgc ccagcacggc tacgtgctgg aaaacggcgc catggcgctg tcgggcaccg
     2521 gcggcgagct gctgggccat ccccgcgtgc tgcaggccta cctggggagc taagcgccgg
     2581 acaggcccgc agcagatgat caaacgactc gcgcatacgc tgtcggcctg gctgcagagc
     2641 ctggcccggc ccatacagtg gctgatcctg ctggcgctat cctccctctt cctgttcgcg
     2701 ctcgaacggg ccgggctgcc ggccggcatc ctgctcggcc cgctcgtggc cggcgccatc
     2761 ctcagcatcc tgggcgtgcg cctgcagctg ccccgccgcc tgggcacgct catccccggc
     2821 gtcgtcggct gcatgatcgt caagctgatg ccttcgtccg gcttcggcca gatcgccgac
     2881 cacctgcccg tctttctctt cggcaccttt gccgtcatca tcgccagcgg cttcctgggc
     2941 tgggtcatga cgcgcatgcg catcctgccc ggcaccacgg ccctctgggg cctgagcccc
     3001 ggcgccgcct cggccatggt gctgatggcc gaggcctacg gcgccgacac ggcgctcgtc
     3061 gccctgatgc aatacctgcg cgtgctgctg gtgacctcga tcacggccag cgtcgccacc
     3121 ctggcgggcg cgtcgcacat gcagcatgcc gccatctcga tgggcagcct gtccgacgtg
     3181 gcctggctgc cgctggccga gaccctggtg ctggccttgg gcggcgcggc cctgggacgc
     3241 gccctgcgcg ttcccggcgg caccatgctc cttcccatgg tgctggcatt cattttcggc
     3301 cactcgggac tggtcgagct ggagctgccc cgcgggctgc tgctggccgt ctatgccctg
     3361 ctgggctggc gcatcggctt tcgcttcacg gcgccgctgc tggcccatgc cgcccgcgcc
     3421 atgccgcgca tcatcctggc cacgctgatc ctgatggcgc tatgcggcgc gctggccggc
     3481 atgctcgtgc tgaccaccgg catcgatccg ctcagcgcct acctggccac cagcccgggc
     3541 ggcgtggatt ccgtggccat catcgccgcc tcgacccacg tcgacgtgac cttcgtgatg
     3601 ggcatgcaga ccgtgcgctt tctcggcgtg ctgctgctcg ggccgatcat ggtcaggctg
     3661 ctggcccgct ccacgcagcg cgacgcccgc cccacctgac cgagcactcg acgccgcgcc
     3721 ccccggtcac cccgaatcgc ccggcataac
//
LOCUS       DASWPO010000249         7939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2650638, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000249 DASWPO010000000
VERSION     DASWPO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2650638"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VGC69_13010"
     CDS             <1..418
                     /locus_tag="VGC69_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0296245.1"
                     /translation="TQAGPLLASLHDWLHDTLRTLSRKSALAEAIRYALARWAALTRY
                     VDDGRIEIDNNAAERALRVVALGRKNYLFAGSDAGGHSAAAIYSLLGTAALNGVEPMG
                     YLREVLARIADHPVNRIQDLLPWHLALPCNAAKQAA"
     gene            373..990
                     /locus_tag="VGC69_13015"
     CDS             373..990
                     /locus_tag="VGC69_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEY0296246.1"
                     /translation="MASGVTLQRSQAGGVSSAKVVPLQGTALVLRIELRHIAPVIYRT
                     VVIPSRITLPKLHVTILRAMGWQGGHLHEFVINSVRYGEPDPNYPEPDLHSEKRVRLE
                     QALKTASSFDYIYDFGDNWEHEVRVLEVASFNGPLDSPWCLEGANACPPEDVGGEPGY
                     MHFLHAISDPTHPEHDDLLLWHGKRFDPSSFDLQEVNQRLMQIRI"
     gene            1031..1672
                     /locus_tag="VGC69_13020"
                     /pseudo
     CDS             1031..1672
                     /locus_tag="VGC69_13020"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1734..2900)
                     /locus_tag="VGC69_13025"
     CDS             complement(1734..2900)
                     /locus_tag="VGC69_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY0296247.1"
                     /translation="MKIAIAGTGYVGLSNGILLAQHHEVVALDIVPEKVEMLNRKQSP
                     IIDADIEDYLQNKPLNFRATLNKREAYQGADYVIIATPTDYDPETNYFNTTSIESVIR
                     DVTAINPKAVMVIKSTVPVGYTTKVGQEMGCKQLIFSPEFLREGRALYDNLHPSRIVI
                     GEQSERAQVFAGLLQEGAVKQDIPVLYTDPTEAEAIKLFANTYLAMRVSYFNELDTYA
                     ATHGLDTRQIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYKSVPQDIIHAI
                     VASNTTRKDFIANDILRRKPKIVGIYRLIMKSGSDNFRASSIQGVMKRIKAKGIEVIV
                     YEPALKETHFFRSRVIQDLDIFKKESDVIISNRMAEALKDVKEKVYTRDLFGSD"
     gene            complement(2952..4313)
                     /locus_tag="VGC69_13030"
     CDS             complement(2952..4313)
                     /locus_tag="VGC69_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0296248.1"
                     /translation="MSLPKTTGLPGLTSALKNKLIQQTLERKLRQAGQEQQPASRPAA
                     APQKKTTISEAYTRFDQYPGYRQLRILNEGAARMGLDSPFFKLHEGTAGAATQIGGKP
                     FINYASYNYLGMSGDPVVMQAAKDAIDRYGTSVSASRLVSGERPIHRELEQALAQVYE
                     VDDAITYVSGHAANVTTIGYLFGPRDLVVHDELIHNSVLQGIQLSGAKRLSFPHNDFE
                     ALDQLLTGQRHQFERVLIVLEGIYSMDGDYPDLPRFVDIKRRHQTFLMVDEAHSMGVM
                     GKTGRGIREHFGLPGNAVDIWMGTLSKTLAGCGGYIAGETALIEHLKFLAPGFLYSVG
                     MPPPVAAASLASLRHMQQHPERVATLQARGHYFLEQAKAAGIDTGSSAGLAVVPAIVG
                     SSLRAAKWSEVLFSQGINVQPILYPAVPEQSARLRFFISCMHSEADIDKTVQGLRALQ
                     QKH"
     gene            complement(4348..>7939)
                     /locus_tag="VGC69_13035"
     CDS             complement(4348..>7939)
                     /locus_tag="VGC69_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0296249.1"
                     /translation="PASLARQALGAEAGQRVADTLRARLAQALQTRAPGQRLGLVEIS
                     ADTPLFAPTLCEGLDFSVADYRCASPDSLALESAERLRERFPALQTQHIGAAAAVPAW
                     QADLVVLHGEFATHDTAQHTLDYALACLKPGGTLLVIGLQPAAWMDFIFGAQASWWLR
                     DEAGAPVSAQWLPSQWQDLLTKHGLRCEDPLELAPDTASGPYLLAATLPAEQIAAPQA
                     APAPSGGWLILADDTPASQGSIAHALAEHLSSTHGLPVILDTQATPETLDALIAQART
                     TFGRIQGIVHAAGLEPRATKKNATALLQAQVQRCALAAGIAQACAHHGLDAALYLLTR
                     GAAPFMPAAGNASNAVLNDASLWLYGRTMANETAGFTVRLVDLPLQGPQPLESLVQEL
                     LWPDAEQEIALGCMGDRHAPRLRKNPRPQPQPRNKPEQKTEPALRLGFDLPGHLRNLR
                     WESHVLPAPAEHELEIRVQATGLNFRDVMYTLGLLSDEAIENGFAGPTLGLEFAGIVE
                     RVGSNVIGYKPGDAVVGFGPASFGNRTLTQANAISRIPPGMDFEAAATIPSTFFTVYY
                     ALHHLARLEPGERILIHGAAGGVGIAAIQYAQWMGAEIHATAGSDEKRDFLRLMGVEH
                     VYDSRSLDFADDVLAATDGQGVDVVLNSLAGEAINRNLRVLKPFGRFLELGKRDFYEN
                     TRIGLRPFRNNISYFGIDADQLMSERPELTRRLFGEMMELFAEGVLHPLPYQVFDANE
                     VVDAFRHMQQARQIGKIVVTYRRGIQGAVIDRRPDKQRPAQLRLPAQATYLVTGGLSG
                     FGLRTAQWLADKGARNLVLASRSGPASDEARQAIADLQARGVTVLAQACDVTRREDLA
                     ALLAHVQATLPPVRGIVHAAMVIDDGLVHNATAEQIERVLAPKILGALHLDELSRNLP
                     LDFFIIHSSGTTLFGNPGQANYVAANGWLEALARQRRARGLPATCPRWGAIADAGFLA
                     RNKKIKDALQSRMGGAALPAADALQALEDMLLARQASPCDLGVLELDWRALSRFLPTA
                     DTPRFAWVARDAVAGSQDDDHSGDIAQLLATLNDAELHPVVVDILRSEVSEILRVPAD
                     KINPERSVYDMGLDSLMGVELITALETRFGIRLPVMALSESPTLAKLATRLIDMLRNE
                     GAAEHDAVAQQVNQVLDQHAQGMSFSSEAIAQFTEDIKTAHNTPKRRMIH"
BASE COUNT         1286 a         2293 c         2809 g         1551 t
ORIGIN      
        1 aacacaggct ggccccttgc tggcatcgct acacgactgg ttgcatgaca cgctgcgcac
       61 gctgtcgcgc aagtcggcgc ttgccgaagc gatccgctat gcgcttgccc gctgggcggc
      121 gttgacgcgc tacgtcgatg acggtcgcat cgagatcgac aacaatgccg ccgagcgcgc
      181 cctgcgcgtg gtcgcgttag gccgcaagaa ctatctgttc gcaggttcgg atgccggcgg
      241 tcacagcgcg gcagccatct acagcttgct gggcacggca gcgctcaatg gggttgagcc
      301 gatgggctac ctgcgtgagg tgctggcccg cattgctgat caccccgtca accgcatcca
      361 ggatctgttg ccatggcatc tggcgttacc ctgcaacgca gccaagcagg cggcgtaagc
      421 agcgcaaagg tcgttccact tcaaggcacg gcactggtat tgcgcatcga gctgcgccat
      481 atcgcgcccg tgatctaccg tacggttgtc atccccagcc gcatcacctt gcccaagctg
      541 cacgtcacga tcttgcgcgc catgggttgg cagggcggac acttgcacga gttcgtcatc
      601 aatagtgtcc gttatggtga acccgatccg aattaccccg aacccgatct gcacagcgag
      661 aagcgggtac gcctggaaca ggcattaaag actgccagca gcttcgacta tatctacgac
      721 ttcggtgaca actgggagca cgaggtgcgt gtactggagg tggcttcctt taacgggccg
      781 ctggactcgc cctggtgctt ggaaggtgcc aacgcctgcc caccagaaga tgtcggcggc
      841 gaacccggct acatgcactt cctgcatgcg atctcggacc ccacccatcc cgagcacgac
      901 gacctgttgc tatggcatgg caagcgcttc gaccccagct ccttcgatct gcaagaggtt
      961 aaccagcggc tgatgcagat ccggatttaa cgccagacgg tattcaccgg acgcttacga
     1021 atcgccttcg tttggctacc gaacggtggc acacctgctg gggttcaaca agaacacggt
     1081 gcagcgggtg ttccagttga tgggctggca ggtcaagaag cgggccattg gcttcaggcc
     1141 gcgtatccag tccatgccct cggtggccac ggcacccaat gagcgatggt cgacggatct
     1201 gtgccggata tgggcaggcc gcgacggttg ggcgacttta gccctggtga tcgattgcca
     1261 tacccgggag ttgctgggct ggcacctgtc cagaagcggc aaggcctgca ccgctggcag
     1321 cgccctggag cacgctctga tcgctcggtt cggtaccttg ggccgggtgc ctgaaccctt
     1381 cttgcttcgg tccgacaatg ggctcgtctt caccagtcgc agctataccg cgctggttcg
     1441 aggctatggg ctgcgccagg agttcatcac gccgcactgc ccgcagcaaa acggcatggt
     1501 cgaacgggtg atccgtacgc ttaaagagca gtgtacccac cggcatcgct tcgagaccct
     1561 gcagcatgcc agccgggtga ttgccgactg gatacgcttt tataaccacc ggcgcccgca
     1621 ccaggccctc ggaatgagaa cccccgctga ggcattcgct ttagcggctt aacctgagca
     1681 ggttccgctg ggtcattaca ttatttgatc cacataaata actaatggaa tttctaatca
     1741 ctgccgaaca gatctcttgt gtaaactttt tcttttacat ctttcagtgc ttcagccatg
     1801 cgattgctta taattacatc ggattctttt ttgaagatat ctaaatcttg aatgacccga
     1861 gagcgaaaaa aatgagtttc cttcaaggct ggctcataca caataacttc aatgcctttg
     1921 gctttgatgc gcttcattac tccctggatg cttgaggctc gaaaattatc tgacccactt
     1981 ttcatgatca agcgatagat ccctacgatt ttaggtttgc ggcgcaggat atcgttcgca
     2041 ataaaatctt ttctcgtagt gttggaggct acgatggcat gaatgatgtc ttgaggtact
     2101 gatttgtagt ttgccagtag ctgcttagtg tccttgggca ggcaataccc accatatcca
     2161 aacgaggggt tgttgtagtg acttccaatc cgtggatcta ggcacacccc gtcgatgatc
     2221 tgccgtgtat ccagcccgtg cgtagctgca taagtatcca gttcattgaa atacgacacc
     2281 cgcatggcca gataagtatt ggcgaaaagt tttatcgcct cagcctcggt ggggtcggtg
     2341 tacagcacgg gtatatcttg cttgactgcc ccttcctgta gcaacccggc aaatacttgg
     2401 gctcgttctg attgctcacc gatcacgatg cgtgagggat gtaggttgtc gtatagcgcc
     2461 cggccttcgc gcaaaaattc aggtgaaaaa atcagctgct tgcagcccat ttcttgaccg
     2521 accttggtgg tatagcccac cggcaccgtg gacttgatca ccatgaccgc tttagggttg
     2581 atggccgtga cgtcccggat gacggactcg atcgaggttg tgttgaagta gttggtctcg
     2641 gggtcgtagt cggtgggggt ggcgatgatg acgtagtcgg caccctggta ggcttcgcgc
     2701 ttattcagcg tggctcggaa gttgagtggc ttgttctgga ggtagtcctc gatgtcggca
     2761 tcgatgatgg gagactgttt gcggttaagc atctccacct tttcgggcac gatgtccagc
     2821 gccaccacct cgtggtgctg tgccagcaag atgccgttgg ataacccaac atacccggta
     2881 cccgctatcg ctattttcat aaattgatcg gtaatgatga agaagaaacg ccgtaaggta
     2941 atgtgcttag gctagtgctt ttgttgcaag gctcgcaacc cctgcactgt tttatcgata
     3001 tccgcttcac tatgcatgca cgagatgaaa aaccgcagcc gcgccgactg ttccggcacg
     3061 gcagggtaga gaatgggctg gacattgatg ccttgcgaga acagcacctc cgaccatttg
     3121 gccgcgcgta gcgagctgcc cacgatggcc gggacgacgg ccaggccggc gctgctgccg
     3181 gtgtcgatgc cggcggcctt ggcctgttcc aggaagtaat ggccgcgtgc ctgcaaggtg
     3241 gccacgcgct cggggtgctg ctgcatgtgg cgcagcgagg ccagggacgc ggcagctaca
     3301 ggcgggggca tgcccacgct gtacaggaag ccgggcgcca ggaacttgag gtgttcgatg
     3361 agcgccgttt cgccggcgat atagccgccg cagccggcca gggtcttgct gagcgtgccc
     3421 atccagatgt ccacggcgtt gccgggcagg ccgaaatgtt cgcggatgcc gcggccggtc
     3481 ttgcccatga cgcccatgga gtgggcttcg tccaccatca aaaaggtttg gtggcggcgc
     3541 ttgatgtcga cgaagcgggg caggtcgggg tagtctccgt ccatgctgta gatgccttcc
     3601 agcacgatga gcacgcgctc gaactggtgg cgttgcccgg tgagcaactg gtcgagcgcc
     3661 tcgaaatcgt tgtgcgggaa ggacaggcgc ttggcgcccg agagctggat gccttgcagc
     3721 acgctgttgt ggatgagctc gtcgtgcacg accaggtcgc gcgggccgaa gaggtagccg
     3781 atggtggtga cgttggcggc gtggccgctg acgtaggtga tggcgtcgtc cacctcgtag
     3841 acctgggcca gggcctgttc gagctcgcgg tggatggggc gttcgcccga gaccaggcgg
     3901 ctggccgaga cggaggtgcc gtagcggtcg atggcgtcct tggcggcctg catgacgacc
     3961 gggtcgcccg acatgcccag gtagttgtag ctggcgtagt tgatgaaggg cttgccgccg
     4021 atttgcgtgg cggcgccggc ggtgccttcg tgcagcttga agaaggggct gtccaggccc
     4081 atgcgggcgg cgccttcgtt gaggatgcgc agctggcggt agccggggta ttggtcgaag
     4141 cgggtatagg cctcggagat ggtggttttt ttctggggcg cggcggcggg gcggctggcg
     4201 ggttgctgtt cctggccggc ctggcgcagc ttgcgctcca gcgtttgctg gatgagcttg
     4261 tttttcaggg cggaggtcag gccgggtagg ccggtagtct tgggaaggct catgtaaggc
     4321 gtagaggatt gggagaagcc atcgtgctca gtggatcatg cgccgcttgg gcgtgttgtg
     4381 ggccgttttg atgtcttcgg tgaattgggc gatggcctcg ctggagaagg acatgccctg
     4441 ggcgtgctgg tccaggacct ggttcacctg ctgggccacg gcgtcgtgct cggcggcgcc
     4501 ttcgttgcgc agcatgtcga tgaggcgagt agccagctta gccagcgtgg ggctttccga
     4561 caaggccatg acgggcaggc ggatgccgaa gcgggtttcc agcgcggtga tgagttccac
     4621 gcccatgagc gagtccaggc ccatgtcgta gacggagcgc tcggggttga tcttgtcggc
     4681 gggcacgcgc aggatctcgc tgacctcgga acgcagtatg tcgaccacca cggggtgcag
     4741 ctcggcgtcg ttcagggtgg ccagcagttg ggcgatgtcg ccgctgtggt cgtcgtcctg
     4801 gctgccggcc acggcgtcgc gggccaccca ggcaaagcgg ggcgtgtcgg cggtgggcag
     4861 gaaccgtgac agcgcgcgcc agtccagttc cagcacgccc aggtcgcacg ggctggcctg
     4921 gcgggccagc agcatgtctt ccagcgcttg cagggcgtcg gcggcgggca gggcggctcc
     4981 gcccatgcgg ctttgcaggg cgtccttgat ttttttgttg cgcgccagga aaccggcgtc
     5041 ggcgatggcg ccccagcgcg ggcaggtggc gggcaggccg cgcgcgcggc gctggcgggc
     5101 cagggcttcg agccagccgt tggcggccac atagttggcc tggccggggt tgccgaagag
     5161 cgtggtgccc gacgagtgga tgatgaagaa gtccagcggc aggttgcggc tcagttcgtc
     5221 caggtgcagg gcgcccagga tcttgggcgc caggacgcgt tcgatctgct cggcggtggc
     5281 gttgtgcacc aggccgtcgt cgatgaccat ggcggcgtgc acgatgccgc ggacgggcgg
     5341 cagggtggcc tgcacgtggg ccagcagcgc ggccaggtct tcgcggcggg tgacgtcgca
     5401 ggcctgggcg agcacggtga cgccgcgggc ctgcaggtcg gcgatggctt gccgggcttc
     5461 gtcgctggcg gggccgctgc ggctggccag caccaggttg cgcgcgccct tgtcggccag
     5521 ccattgcgcc gtgcgcaggc cgaagccgga caggccgccg gtgacgagat aggtcgcctg
     5581 ggcgggcagg cgcaactggg ccgggcgttg cttgtcgggc cggcggtcga tgacggcgcc
     5641 ctggatgccg cggcgatagg tgacgacgat cttgccgatc tggcgcgcct gctgcatgtg
     5701 gcggaaggcg tcgaccacct cgttggcgtc gaagacctgg tagggcagcg ggtgcagcac
     5761 gccttcggcg aaaagctcca tcatttcgcc gaagaggcgc cgggtgagct cggggcgttc
     5821 ggacatgagc tggtcggcgt cgatgccgaa gtagctgatg ttgttgcgga aagggcgcag
     5881 gccgatgcgg gtgttttcgt agaagtcgcg cttgcccagt tccaggaagc ggccgaaggg
     5941 cttgagcacg cgcaggttgc ggttgatggc ttcgccggcc agcgagttga gcactacgtc
     6001 cacgccctgg ccatcggtgg cggccagcac gtcgtcggcg aagtccagcg agcgcgagtc
     6061 gtagacgtgt tccacgccca tcaggcgcag gaagtcgcgc ttttcgtcgg agcccgcggt
     6121 ggcgtggatc tcggcgccca tccattgggc gtactggatg gcggcgatgc ccacgccgcc
     6181 ggccgcgccg tggatgagga tgcgctcgcc gggttcgagg cgcgccaggt ggtgaagcgc
     6241 gtagtagacg gtgaagaagg tgctggggat ggtggccgcg gcttcgaagt ccatgcccgg
     6301 cgggatgcgc gagatggcgt tggcctgcgt gagggtgcgg ttgccgaagc tggccgggcc
     6361 gaagcccacc acggcgtctc ccggcttgta gccgatgacg ttgctgccca cgcgttcgac
     6421 gatgccggcg aattccaggc ccagcgtggg gccggcgaag ccgttttcga tggcttcgtc
     6481 cgacagcagg cccagggtgt acatcacgtc gcggaagttc aggccggtgg cctgcacgcg
     6541 gatctccagc tcgtgctcgg cgggcgcggg caggacgtgg ctttcccagc gcaggttgcg
     6601 caagtggccg ggcaggtcga agcccaggcg cagggcgggt tcggtttttt gctcgggctt
     6661 gttgcgcggc tggggctgcg ggcgcggatt cttgcgcagg cgcggcgcgt ggcgatcgcc
     6721 catgcagccc agggcgattt cctgctcggc atcgggccac agcagttcct gcaccagcga
     6781 ttccagcggc tgcgggccct gcaggggcag gtcgaccagc cgcacggtga agcccgcggt
     6841 ttcgttggcc atggtgcggc cgtacagcca gagcgaggcg tcgttgagca cggcattgct
     6901 ggcgttgccg gcggccggca tgaagggggc cgcgccgcgg gtgagcaggt atagcgcggc
     6961 atccaggccg tggtgcgcgc aggcctgggc aatacccgcg gcgagggcgc agcgttgcac
     7021 ttgggcttgc agcagcgccg tggcgttctt cttggtggcg cgcggctcca ggccggcggc
     7081 gtgcacgatg ccctggatgc ggccgaacgt ggtccgcgcc tgcgcgatga gcgcgtccag
     7141 ggtctcgggc gtggcctgcg tgtcgaggat gacgggcagg ccgtgggtgg acgagaggtg
     7201 ttcggccagc gcatgggcga tgctgccctg gctggccggc gtgtcgtcgg ccaggatcag
     7261 ccagccgccg ctgggcgccg gcgcggcttg cggggcggcg atttgctcgg cgggcagggt
     7321 ggcggccagc agataagggc cgctggccgt gtcgggggcc agttcgagcg ggtcttcgca
     7381 acgcaggccg tgtttggtca gcaggtcttg ccactgggag ggcagccatt gcgccgacac
     7441 cggggcgccg gcttcgtcgc gcagccacca cgaggcttgg gcgccgaaga tgaagtccat
     7501 ccaggcggcg ggctgcaggc cgatgacgag cagcgtgccg cccggtttca ggcaggccag
     7561 cgcgtagtcc agggtatgtt gcgcggtgtc gtgcgtggcg aattcgccgt gcaggacgac
     7621 aaggtcggcc tgccatgccg gcacggccgc ggcggcgccg atgtgctggg tttgcagcgc
     7681 gggaaagcgt tcgcgcaggc gttcggcgga ttccagcgcc aggctgtcgg ggctggcgca
     7741 gcggtagtcg gccacggaaa agtccaggcc ttcgcacagc gtgggcgcga acagcggcgt
     7801 gtcggcgctg atctcgacca gccccagccg ttggccgggc gcgcgggtct gcagggcctg
     7861 ggccaggcgc gcgcgcaggg tatcggccac gcgctggccg gcctcggcgc ccagggcctg
     7921 gcgcgccagc gaggcgggc
//
LOCUS       DASWPO010000250         6838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2023764, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000250 DASWPO010000000
VERSION     DASWPO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023764"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VGC69_13040"
     CDS             complement(<1..385)
                     /locus_tag="VGC69_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296250.1"
                     /translation="MSADPATAALPASPTLDSARLSRAARMIPFIVGCALFMQMLDST
                     VVATALPAMAASLGSTPVRLNVAITAYLLAVAVFVPVSGWAADRYGARRVFITAIALF
                     TLSSIGCAMSQNVTQLVLARIVQGMA"
     gene            532..720
                     /locus_tag="VGC69_13045"
     CDS             532..720
                     /locus_tag="VGC69_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEY0296251.1"
                     /translation="MAQNYESEATQFIKAYKAQHPGTEQRQLEGRARLWDKAQDAELL
                     DGFRQARVPQKPYVYQAD"
     gene            745..1629
                     /locus_tag="VGC69_13050"
     CDS             745..1629
                     /locus_tag="VGC69_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY0296252.1"
                     /translation="MSGNPVIPGEDLAALVEPPVDTTPDVVDTVAFARLYGEPLFQLP
                     NDLYIPPDALEVFLEAFEGPLDLLLYLIRKQNFNVLDIPMADVTRQYLSYVEQIRVHN
                     LELAAEYLLMAAMLIEIKSRMLLPLRKTDTGEEPEDPRAELVRRLLEYEQMKLAAQKL
                     DALPQIGRDFVHSQAVAEISVERMMPEVDVDDLRAAWADIMKRARLNQHHQITREQLS
                     VRDHMTHILRRLNEVRFIEFGELFMERIREGAPTAVVVVHFIAMLELARESLLEITQA
                     EPYAPIYVRLAYTSAVAA"
     gene            1673..2527
                     /gene="panC"
                     /locus_tag="VGC69_13055"
     CDS             1673..2527
                     /gene="panC"
                     /locus_tag="VGC69_13055"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY0296253.1"
                     /translation="MKVVHSIQDLRDQLRGQNRVAFVPTMGNLHDGHLALMKLARQHG
                     DPVVTSIFVNRLQFGPNEDFDRYPRTLRDDVEKMEQRRDVYLAFAPDEREMYPEPQSY
                     RVQPPDDLSDILEGEFRPGFFSGMCTVVMKLLSCVQPRVAVFGKKDYQQLMIVRSMCR
                     QFQLPVEILAHETVRADDGLALSSRNRYLSQEERAEAPQLYATLQAMRDGLRAQLAAG
                     APDLAAIEGEAVRALQARGWQADYMSVRRQRDLKAPEADEVKAGEPLVALAAAKLGVT
                     RLIDNLEF"
     gene            2676..2918
                     /locus_tag="VGC69_13060"
     CDS             2676..2918
                     /locus_tag="VGC69_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY0296254.1"
                     /translation="MPCGTATAPFPAPRLARLSARERELVGHFARGRPNKVVAIDFGI
                     SERTAEAHRARIFRKMGVRSALELACCICPHRPPGG"
     gene            complement(2951..3763)
                     /locus_tag="VGC69_13065"
     CDS             complement(2951..3763)
                     /locus_tag="VGC69_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296255.1"
                     /translation="MPSFLLPTLRTLTLTVAISGSLLTTGCAQLSNLSSPGYYDPPAE
                     SSSTDAAFQGQGGASRNMVVRPPSQIQFSLPNPSNPRKPAQDTPTAVADSGAPPVAVG
                     SPVPDENTAAPPVPAAGANARMKDPAAASPAPATDHPAQRAFLPQAETFVGTFPCLSP
                     SAQCEAQRVTLTLGPNGRWRSRAASLAGQAQAAAPQAEQGCWNVTGDQPAHVLLLDVQ
                     GNAHAELLASANNVLSVKAILGRAPTLSYTLTRQPDLDPIDELSKQPLPACE"
     gene            3923..4648
                     /locus_tag="VGC69_13070"
     CDS             3923..4648
                     /locus_tag="VGC69_13070"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006228008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY0296256.1"
                     /translation="MWQVFPIAQGWAMALAGLVGLAAGWGLSALSRGLPRRLEQAWQA
                     QAGGLAEALPAAPDAGRRADWLLDARGGAMALLTALLFAACAWRLGPTPVAVCGMALA
                     AALVALAWIDAETGLLPDVLTLPLVWLGLLVNLHGALAPLPQAVLGAAVGYLFLWVIF
                     HAFRWITGVEGMGYGDFKLMAALGAWFGLAATPWLLLGAAAAGAVAGLGLRLSGRLGR
                     GQALPFGPYLAVGGILMLLAGVR"
     gene            4708..5346
                     /gene="coaE"
                     /locus_tag="VGC69_13075"
     CDS             4708..5346
                     /gene="coaE"
                     /locus_tag="VGC69_13075"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391306.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY0296257.1"
                     /translation="MLSIGLTGGIGSGKSRVADLLAQWGAAIIDSDAIAHALTAPGGA
                     AMPALREAFGEQAVTADGALNRAWMRDLVFRDPAARGRLEGILHPRIAQHTIEQGELA
                     RGPYRVFVVPLLVESGRWRDRLERICVVDCDPQTQVARVQARSGLTPDGIRAIMAAQS
                     TREARLRAADDVILNDGATTPAMLHERTKLMHDRWIALAAAQDNSTGRQASA"
     gene            5408..6160
                     /gene="zapD"
                     /locus_tag="VGC69_13080"
     CDS             5408..6160
                     /gene="zapD"
                     /locus_tag="VGC69_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEY0296258.1"
                     /translation="MTLYEYPFNERVRAYLRLEYLFDRLFFLVGCDDTRAHQIAVSTL
                     FDILDALERTDVKGSILQDIERQRMGLTPLRGHPEVAQDALETMLGDMERVIGVLTSQ
                     GKTGQALRENEWLASLRGRLSVAGGSTQMDVPSFYAWQQKPIEARRVDLARWIDTLMG
                     LCEGLALVLRLLRENGVKSQAVAEQGAYQQMLGGKTYQLLRIWVDPAQGVFPEISANK
                     YMIWVRYATQDGELKPQSVARDVPFNMSLCTV"
     gene            6409..>6838
                     /locus_tag="VGC69_13085"
     CDS             6409..>6838
                     /locus_tag="VGC69_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0296259.1"
                     /translation="MSVISPPQTRRRRRWPVVLAIIVLLLIVMVAIHLFTRKAPQRMS
                     LAQVVTVAEVRTGDMPETLAALGTVTPEATVTVLPQLSGFLTEVGYQEGQTVQKGQFL
                     AQIDPRQYQIGKQQAEAQLARDQATLDQARADLARYTQLHN"
BASE COUNT         1041 a         2278 c         2429 g         1090 t
ORIGIN      
        1 cggccatgcc ctgcacgatg cgcgccagca ccagctgcgt gacgttctgc gacatggcgc
       61 agccgatcga gctcagggtg aacagggcga tggcggtgat gaacacgcgc cgggcgccgt
      121 agcggtcggc cgcccagccg ctgaccggca cgaacacggc cacggccagc aggtaggcgg
      181 tgatcgccac gttcagccgc accggcgtcg agcccaggct cgccgccatg gcgggcaggg
      241 cggtggccac cacggtggag tccagcattt gcatgaagag cgcgcacccc acgatgaagg
      301 ggatcatgcg tgcggcccgg gatagccgtg ccgagtcgag agtgggggaa gcgggcaggg
      361 cggcggtggc tgggtcggcg gacatggggg agctgcggcg tgcggcggct ggcagtggtt
      421 ctcccgattg taacgccggg ggcggggcgc cccgcgcggc cgtgaagtaa actccggcaa
      481 aggttccctg gcagccccgg ctgccgttac aactgtagcg atccccgcac catggcccaa
      541 aactacgaat ccgaagcgac ccagttcatc aaggcctaca aggcgcaaca ccccggcacc
      601 gagcagcgcc agctcgaagg ccgcgcccgc ctgtgggaca aggcccagga cgccgaactg
      661 ctcgacggtt tccgccaggc ccgcgtgccg cagaagccct acgtctacca ggccgactga
      721 accggccggg ttttcgacga cacgatgtcc ggcaaccctg tcatcccggg cgaggacttg
      781 gccgcgctgg tcgagccgcc ggtcgacacc acgcccgacg tggtcgatac ggtggctttc
      841 gcgcggctgt acggcgagcc gctgttccag ctgcccaatg acctgtacat cccgccggac
      901 gcgctcgagg tctttctcga ggccttcgag ggcccgctgg acctgctgct ctacctgatc
      961 cgcaaacaaa acttcaacgt gctcgacatt cccatggcgg acgtcacgcg gcagtacctg
     1021 tcgtatgtcg agcagatccg cgtgcacaac ctcgaactgg ccgccgagta cctgctcatg
     1081 gcggccatgc tgatcgagat caagtcgcgc atgctgctgc cgctcaggaa gaccgacacc
     1141 ggcgaagagc ccgaggatcc gcgcgccgaa ctggtgcgcc gcctgctgga gtacgagcag
     1201 atgaagctcg ccgcgcagaa gctcgatgcc ttgccgcaga tcgggcgcga ttttgtgcac
     1261 agccaggcgg tggccgagat cagcgtcgag cgcatgatgc ccgaggtcga cgtcgacgac
     1321 ctgcgcgcgg cctgggccga catcatgaag cgcgcgcggc tgaaccagca tcaccagatc
     1381 acgcgcgaac aattgtcggt gcgcgaccat atgacccaca tcctgcgccg cctgaacgaa
     1441 gtgcgcttca tcgagttcgg tgaactcttc atggagcgca tccgcgaagg cgcccccacc
     1501 gccgtggtgg tggtgcactt catcgccatg ctcgaactgg cgcgcgagtc gctgctcgag
     1561 atcacccagg ccgaacccta cgcgcccatc tacgtgcgcc tggcctatac cagcgccgtc
     1621 gcagcctgag gctgctccgt ccattgcccg gcggccctgc ccggagaatt ccttgaaagt
     1681 cgtacacagc atccaggacc tgcgtgacca actgcgcggc cagaaccgcg tcgccttcgt
     1741 gcccaccatg ggcaatctgc acgacggcca tctggcgctg atgaagctgg cgcgccagca
     1801 cggcgacccg gtggtcacca gcattttcgt caaccgcctg cagttcggcc ccaacgagga
     1861 tttcgaccgc tatccgcgca cgctgcgcga cgacgtcgag aaaatggagc agcgccgcga
     1921 cgtgtacctg gccttcgcgc ccgacgagcg cgagatgtac cccgagccgc agagctaccg
     1981 cgtgcagccg cccgacgacc tgagcgacat cctcgagggc gagttccggc ccggcttttt
     2041 ctcgggcatg tgcacggtgg tgatgaagct gctgtcgtgc gtgcagccgc gcgtggccgt
     2101 gttcggcaag aaggactacc agcagctcat gatcgtgcgc agcatgtgcc ggcagttcca
     2161 gctgccggtg gagatcctgg cgcacgagac cgtgcgggcc gacgacggcc tggccctgtc
     2221 ttcgcgcaat cgctacctga gccaggaaga gcgcgccgag gcgccgcagc tctatgccac
     2281 cctgcaggcc atgcgcgatg ggctgcgcgc ccagcttgcc gccggcgcgc cggatctggc
     2341 cgccatcgag ggcgaggccg tgcgcgcgtt gcaggcgcgc ggctggcagg ccgactacat
     2401 gtcggtgcgc cgccagcgcg acctcaaggc gcccgaggcg gacgaggtca aggccggcga
     2461 gccgctggtg gcgctggccg ccgccaagct cggcgtcacc cgcctgatcg acaacctcga
     2521 attctgatgc ggccggcggc cgccggccgc cccttcgctc cgtcggaaaa gcgcggcgcc
     2581 tgcgtcgaaa acggctctgt ctgcgagaat tgacaggtgc cgcccgcggc cgcgcatgcc
     2641 acactcgccg cgtccatttg cgaacggggg tgtgcatgcc gtgcggtacc gcaacagcgc
     2701 cttttccggc gccgaggctg gccaggttgt cggcgcgcga gcgcgagctg gtcggtcatt
     2761 tcgcccgggg gcggcccaac aaggtggtcg ccatcgattt cggcatttcc gagcgcacgg
     2821 ccgaggcgca ccgcgcgcgc atctttcgca agatgggcgt gcgcagcgcg ctggagctgg
     2881 cgtgctgcat ttgcccgcat cgcccgcccg gcggctgatg cctgcggcgg gcgatgccga
     2941 tccgcaagcc ctattcgcag gccggcagcg gctgcttgga caattcgtcg atgggatcga
     3001 ggtcgggctg gcgcgtgagg gtgtaggaca gcgtgggcgc gcgtcccagg atggccttca
     3061 cgctcagcac gttgttggcc gaggccagca gctcggcgtg cgcattgccc tggacgtcga
     3121 gcagcagcac atgggccggc tggtcgcccg tgacgttcca gcagccctgc tcggcctgcg
     3181 gcgcggccgc ctgggcctgg cctgccaggg aggcggcgcg gctgcgccag cggccgttgg
     3241 ggcccagggt gagggtgacg cgctgggctt cgcattgcgc cgacggcgac aggcagggaa
     3301 aggtgccgac gaaggtttcc gcctgcggca ggaaggcgcg ctgcgccggg tggtcggtgg
     3361 ccggcgcggg gctggcggcg gccgggtcct tcatccgggc attggcgccg gcggccggca
     3421 cgggcggcgc cgcggtgttt tcgtccggaa cggggctgcc gacggcgacc gggggggcgc
     3481 cgctgtcggc aacggcggtg ggggtgtctt gcgcgggctt gcgcgggttg gaggggttgg
     3541 gcaggctgaa ctggatctgc gagggcgggc gtaccaccat gttgcggctg gcgccgccct
     3601 gcccctggaa ggcggcatcg gtgctggagc tctcggccgg cgggtcgtaa tagcccgggc
     3661 tgctcaggtt gctcaactgg gcgcagccgg tggtcagcag cgagccgctg atcgccaccg
     3721 tgagagtcag ggtgcgcagg gtcggcagaa ggaaagatgg catggaggga gtccggaggg
     3781 cgtgtgagta gcgtattgtt cggccatgct aggccgctag cgttctggat ggaattttac
     3841 gcacttggtg cctgcgtgcc actgccgcgg gccggcgccg gagtgcccgc accgtgattt
     3901 ggacaggatg tcgagttttt tcatgtggca agtctttccc attgcccagg ggtgggccat
     3961 ggcgctggcc gggctggtcg ggctggcggc cggctggggg ctgagcgcgc tgtcgcgcgg
     4021 cctgccgcgc cggctcgagc aggcctggca ggcgcaggcc gggggccttg ccgaggcgct
     4081 gcccgcagcc ccggatgcgg gccggcgcgc cgattggctc ctcgacgccc gcggcggcgc
     4141 catggcgctg ctgacggcgc tgctgtttgc cgcctgcgcg tggcgcctgg ggccgacgcc
     4201 cgtcgccgtg tgcggcatgg cgctggcggc ggccctggtg gccctggcct ggatcgacgc
     4261 cgaaaccggc ctgctgcccg acgtgctgac gctgcccctg gtgtggctcg ggctgctggt
     4321 gaacctgcac ggagcgctgg cgcccttgcc ccaggcggtg ctgggcgcgg ccgtggggta
     4381 tctgttcctg tgggtgatct tccatgcgtt tcgctggatc accggggtcg agggcatggg
     4441 ctatggcgat ttcaagctca tggccgcgct gggagcctgg ttcgggctgg ccgcgacgcc
     4501 ctggctgctg ctgggcgcgg cggcggcggg agcggtggcc ggcctgggcc tgcgcctgag
     4561 cggccggctg ggccgaggcc aggccctgcc gttcggcccc tacctggcgg tgggcggtat
     4621 cctgatgctg ctggccgggg tgcgatgagt catgcggccc ggtttggcta tttttgacgg
     4681 catggacgca atatgggaaa agccaggatg ttgagcatcg gactgaccgg cggcattggt
     4741 tcgggcaagt cgagggtggc ggacctgttg gcgcaatggg gcgccgcgat catcgacagc
     4801 gacgcgatcg cgcatgcgct cacggcgccg ggcggcgcgg ccatgccggc cttgcgcgag
     4861 gccttcggcg agcaggccgt gacggccgat ggcgccctga atcgcgcgtg gatgcgcgat
     4921 ctggtgttcc gcgacccggc cgcgcggggc cgcctcgaag gcatcctgca cccccggatc
     4981 gcccagcaca cgatcgagca gggcgagctc gcccgcgggc cgtaccgggt gttcgtggtg
     5041 ccgctcctgg tcgaatccgg ccgctggcgc gacaggctcg agcgcatttg cgtggtcgat
     5101 tgcgacccgc agacccaggt ggcccgggtg caggcgcgca gcgggctgac gccagacggt
     5161 atacgggcta tcatggcggc acagtccacg cgtgaggcta ggctgcgggc ggcggacgac
     5221 gtcattctca atgacggcgc taccacgcct gccatgctgc acgagcggac caagctgatg
     5281 catgatcgtt ggatcgccct ggccgcagca caagacaact ctaccggccg gcaagcatcc
     5341 gcctgataat cccggccctg gagcctcgcg cgaggtccat cggtcgatcg tcatgggcct
     5401 gcggcgagtg actctgtacg aatatccttt caacgagcgt gtccgtgcct acctcaggct
     5461 ggagtacctg ttcgacaggc tgttcttcct ggttggctgc gacgacacgc gcgcgcacca
     5521 gatcgcggtt tccaccctgt tcgacatact cgatgcgctc gaacgcaccg acgtcaaggg
     5581 ctccatcctg caggatatcg aacgccagcg catggggctg acgccgctgc gcggccatcc
     5641 cgaggtcgcc caggacgcgc tcgagaccat gctgggcgac atggagcgcg tcatcggcgt
     5701 gctgacctcg cagggcaaga ccggccaggc gctgcgcgaa aacgaatggc tggccagcct
     5761 gcgcgggcgc ttgtcggtgg ccggcggctc cacccagatg gacgtgccct cgttctatgc
     5821 ctggcagcag aagccgatcg aggcgcgccg ggtcgacctg gcgcgctgga tcgacacgct
     5881 catgggcctg tgcgagggcc tggccctggt gttgcggctg ctgcgcgaaa acggcgtcaa
     5941 gagccaggcc gtggccgaac agggcgccta ccagcagatg ctgggcggca agacctacca
     6001 gctgctgcgc atctgggtcg acccggccca gggcgtgttc cccgagatca gcgccaacaa
     6061 atacatgatc tgggtccgct atgccaccca ggacggcgag ctcaagccgc agtcggtggc
     6121 ccgcgacgtg cctttcaaca tgtcgctttg cacggtctag gggttcccga gccctcggtc
     6181 ggcgcgccaa ctggtttcat cttattacaa aaaccaggct tggtaatcag tcggaacgtg
     6241 ccctcagggc aacgattcga aaaacccctg cacctcggct agactcgcca ctttgcttcc
     6301 cgcgacaact cccattgccg ccggtcggct tttgcttttt tcctgtcatt gcccccatgc
     6361 ccgggcgccc ggtccggccg ggcccttgcc gccataaggt cgaaagagat gagcgtaatc
     6421 agtccaccgc aaaccaggcg acgccggcgc tggcccgtcg tgctggcgat catcgtgctg
     6481 ctgctgatcg tcatggtggc catccatctg tttacgcgca aggcgcctca gcgcatgtcc
     6541 ctcgcccagg tggtgacggt ggccgaagtg cgcacgggcg acatgccgga aaccctcgcg
     6601 gcgctgggca cagtcacgcc cgaggccacg gtcaccgtgc tgccgcagct cagcggcttc
     6661 ctgaccgaag tgggctacca ggaaggccag acggtgcaaa agggccagtt cctggcgcag
     6721 atcgacccgc gccagtatca aataggcaag cagcaggccg aggcccagct cgccagggac
     6781 caggcgacgc tcgaccaggc gcgcgccgac ctggcccgct acacgcaatt gcacaacc
//
LOCUS       DASWPO010000251        34760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2918287, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000251 DASWPO010000000
VERSION     DASWPO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 34760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34760
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2918287"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VGC69_13090"
     CDS             complement(<1..289)
                     /locus_tag="VGC69_13090"
                     /inference="COORDINATES: protein motif:HMM:NF027545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4880 domain-containing protein"
                     /protein_id="HEY0296260.1"
                     /translation="MHCASSTERAVLGGGAEDIPARVVDAAVAWAVKLNYNEPDAATR
                     QEFEHWLQAGPLHRAAWQRLQALRDEMHAVPPSLALDTLRAKDMRRIASRRR"
     gene            complement(267..776)
                     /locus_tag="VGC69_13095"
     CDS             complement(267..776)
                     /locus_tag="VGC69_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0296261.1"
                     /translation="MISSVNPIEALYADHHGWLVAWLRGKLGCSHQAADLAQDTFLRV
                     LLKRQEDLQKGLKSPRAYLTTIAHGLLVEHWRRRDLEHAWLATLAQLPEPQVPSPEVR
                     LILLEALDRIDAMLSQLKPAVRSAFLWAQLEGASCPEIARRLGVSLATAERYVATALR
                     HCYALRFEY"
     gene            1001..1076
                     /locus_tag="VGC69_13100"
     tRNA            1001..1076
                     /locus_tag="VGC69_13100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1034..1036,aa:Gly,seq:gcc)
     gene            1313..2449
                     /locus_tag="VGC69_13105"
                     /pseudo
     CDS             1313..2449
                     /locus_tag="VGC69_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            2598..2849
                     /locus_tag="VGC69_13110"
     CDS             2598..2849
                     /locus_tag="VGC69_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY0296262.1"
                     /translation="MQTFTANEAKTRFGELIDRVQREPIQVTRRNRVVGVMVSAEDYQ
                     AMREFYADRLRRTLKQSAEEAAGKGLTDEKLEQLLANED"
     gene            2839..3267
                     /locus_tag="VGC69_13115"
     CDS             2839..3267
                     /locus_tag="VGC69_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEY0296263.1"
                     /translation="MRIDRAVIDTNVLISAALSPRSAPAQIVDCLLQHATLVFSRETF
                     EELETRLWRPKFDRYLDMDRRRLLLHDFAAAARWVDLPPTPRPEYSRDPDDDVFLHTA
                     LHGKAEWLVSGDKDLLELPPADWGFEILSPAQALEKWHQK"
     gene            3962..4528
                     /locus_tag="VGC69_13120"
     CDS             3962..4528
                     /locus_tag="VGC69_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0296264.1"
                     /translation="MAISEFTPERQIEALYADHHGWLLGWLRRRLGGGVHDAADVTQD
                     TFVRILASRDALLGVKEPRAYLTTIATRLLVDRSRRELVERTYLAEMALLVENPPSYP
                     APDDVLMAVQALEQIGAALEGVPPKVREAFLRHYLDEQTHAAIAADLGVSKRMVQKYL
                     VQALLHCRARCPAMAEHGAKPAGAKDVS"
     gene            4525..5568
                     /locus_tag="VGC69_13125"
     CDS             4525..5568
                     /locus_tag="VGC69_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0296265.1"
                     /translation="MTQPESRPDLPDETDLLTEQAAQWIVLLTDDDEATRTQARAGFE
                     RWKQADTRHAAAAASLEQLIGQVRGVRETAGGSARPARAALDAVFADRRHRQRLKRAG
                     AALLAVFVLAAPAWMALRAWPPAYAMADIRTATGQWETRLLSDGTRITLDSASAVDLH
                     YDAHRRAIELVQGEILVDVAKDAARPFLVETTHGSIRALGTRFVVGHQDDATVLSMIE
                     SRVAVQTARQRAAGSSETTEVVAGQRVRITENAVGPAEPIDTRSLADAWKYHQLVVDN
                     RPLPEVLDELAHYRPGHIHYDLAEIEPIRVSAVLPLDDPDQALQLLHIKFPALRIRTV
                     TPWLVLVDAPSPK"
     gene            5754..8210
                     /locus_tag="VGC69_13130"
     CDS             5754..8210
                     /locus_tag="VGC69_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0296266.1"
                     /translation="MSVSPVSAQVSAEPSLRARPTPLALAVHAAFATALALGFIQCSQ
                     AQTPAQAAQYDVPAGPLAEAVNRFAQQSGVSIVMDANQIRGLTSPGLRGNYGVQEGFD
                     MLLRGSGYTAGKTPAGYVLRPTPPSTSGMRGESPAVMPAVSVVGARPSDELPPAYAGG
                     QVATGGRVGLLGNRDMMDTPFSVVSYTSKTIEDHQAQSIGDALMNDPSVRVDRPAGSY
                     ADEVYIRGFPLMNPDIFFNGLSGVLPYNFVGPEFLERVELLRGASGLLYGISPNGAVG
                     GAINVVPKRATDDPITRLTTTYSSDSQFGTHVDVGRRFGEDNRWGIRFNGTFRDGDMS
                     ANNTSERLGAGMLGLDYRGDRFRFSLDGGYQNRRLKGFFDSLDFLSNDFKVPSAPKAS
                     DNYPSWTRFNSEDTFGMARAEYDIAPDWTVFAAAGFSHNNSSTLGGVGLLENSAGDAL
                     FFPRANKFTEDSASTQVGIRGKFDTGPIGHQVSLTGDYYRHRAAFGQTLFASQDVNIY
                     DPGSFSAPDLSSYSPNAPLSSITENSSVAAADTLSILDDRIQLTAGLREQHIDTRNYA
                     ATGTVSSEYDRSVLTPAVAFVVKPWERVSLYANYIEGLQPGAVVPATYANGGQALPPY
                     VSRQYEIGTKVDWGKVTTTLSLFQIKQPSGSADPLTNIYSANGEQRNRGIELQAFGEA
                     AQGVRLLGGIALTDARLTQTNSPATQGNRPYGVPDVQANLGVEWDTPFVQNLTLTGNA
                     VYTSSQYIDATNQQKIPSWIRFDVGARYVWQRENGKPITFRAQILNVTNRRYWSSYSF
                     TNSLAEGAPRTVMLSAQFDF"
     gene            8412..8987
                     /locus_tag="VGC69_13135"
     CDS             8412..8987
                     /locus_tag="VGC69_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0296267.1"
                     /translation="MPNEATPAEFESLYGSHHGWLQGWLHRRLGNAADAADLAHDAFL
                     RLMQTPRRFDSAPQARSYLRTMANGLCVDLWRRRSIEQAWLDALAAQSEAVAPSAEHQ
                     AIVLEALQEIDAMLHSLPPKAAQAFVLAVAGGMTHKEVARELGVSTRTVTTYITQAML
                     HCLHLEARQVVQELPYGARMPASASPIPSAL"
     gene            9018..10040
                     /locus_tag="VGC69_13140"
     CDS             9018..10040
                     /locus_tag="VGC69_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0296268.1"
                     /translation="MATASVGAPSCVPSHRALQQAAEWFALLDSGEATATDRGRWDAW
                     LAESAEHREAWRFVERISRRFDPIKASPERRTALTAYQLAHGIRIRRRQTLLSLAALT
                     GSGLLGWTVWRQASVPELALSWVADYRTGTGEIHGIVLADGTRVWLNAASAFNQDYQP
                     DQRKLRLVRGEILVDTAADPLGRPFYVETSQGQMQALGTRFTVRLDGSETFLAVYEGS
                     VEVRTADTGAMAVIPAGRQIRFTRAALAASETADPAREAWTRGLLVARDIPLAEVVQE
                     LRRYYPGHLSLAPEVADLRVLGGYPVNDPDKVLAMLESVLPIRVQRTLPWWVSIEPRQ
                     EAASSR"
     gene            10404..12797
                     /locus_tag="VGC69_13145"
     CDS             10404..12797
                     /locus_tag="VGC69_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY0296269.1"
                     /translation="MHLALAGGLLAARQPAAHAQAAAAQPSQALAARAYDIPSGPLEA
                     ALTRFVTESGMPLAATPGLVQGKQSPGLHGTFGAQAALNALLAGTGLEAVVGNEGSYV
                     LRQAPAETSGAGIATLPAIAVTGVATVDLPPVYAGGQVASGGQLGFLGNRSIFDTPFN
                     QSSYTSQLIQDQQARSIADVAANDPSVRQAWVSGSYTDQFYIRGFPVGASDVAVNGIF
                     GLAPFQYSNTDWVDRVEILKGTNALLNGMSPGGSVGGNINLVPKQAEDTPITQLTTSY
                     MSRGQLGTNVDIGRRFGTNQEWGIRFDGTYKNGDTSRDSQRQELGSAFLGLDYRGERF
                     RMSLDAGYQHQDFASPTRFTYLGAGVQVPSAPDGSNNWEPSWTYVKSDDTFGLIHGEY
                     DLTNNWTLYGSFGARRNDFKGVYGQSTITDSTGDFTGTYYTQPTWAESYSGQIGVRGH
                     LTTGPVEHDIDLGYSNLDWNFGGLLTYLPGTYSSNIYSPVSLSQPDLSSVNLHVFRTS
                     HILLRSYAFADTLSILDKRVQLTLGGRYQEIGVENFDQNTGILSSSYDRRALTPAVGL
                     VVKPWQKVSLYANYIEGLQQGPTAPTGSTNVGEVFAPIKSRQMEAGVKVDFGHIGTTL
                     SVFQIKQPSGITNPTTFHYDINGEQRNRGVEFNTFGEVYPGVRILGGVAFIDAQMTKT
                     AGGTLDGKRPVGVPTFQANIGAEWDTPFVKGLTLSTRLVHTNGAYVDSTNTRATSGWE
                     RVDIGARYTFHRSGGKTIILRAGIDNIFNKNYWSATQFGMLELGEPRTYRLSATFDF"
     gene            complement(12896..14107)
                     /gene="zigA"
                     /locus_tag="VGC69_13150"
     CDS             complement(12896..14107)
                     /gene="zigA"
                     /locus_tag="VGC69_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HEY0296270.1"
                     /translation="MKTNVSAALPRLPVTVLSGFLGAGKTTLLNHILNNREGRRVAVI
                     VNDMSEVNIDAALVREGGAELSRTDEKLVEMSNGCICCTLREDLLVEVSRLAKEGKFD
                     QLVIESTGISEPLPVAETFTFEGDDGRSLGEVARLDTMVTVVDAYNFLRDYGSRDSLQ
                     DRGESLGEEDERTVVDLLVDQIEFCDVIILNKADLINEAERERLMAILRKLNPRARIE
                     VSEFGKVPLDRVLNTALFDFDEASKAPGWLRELRGQHTPETEEYGIRSFVYRARRPFH
                     PERFFAFVESEWPGVVRSKGHFWLASHPELAGSWSQAGAVARHGAAGYWWAAMPPERW
                     PQDPEYVALIRRNWVDGVGDARQELVLIGMDMDEAALRARFEACLLTDKEMAQGPLAW
                     KTWRNPFPNWP"
     gene            complement(14591..15403)
                     /locus_tag="VGC69_13155"
     CDS             complement(14591..15403)
                     /locus_tag="VGC69_13155"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0296271.1"
                     /translation="MEYQTLVCAREDWIATVTLNRPDRMNALTAIMEAELRDAMEAAD
                     ADPLVRVIVLTGAGRAFCAGMDMDELEVLAPDDIRQQRWMRPYDMNRRADYQSRYGYF
                     PALRKPVIAAINGAAAGLGLVMALYSDVRFASTAAVFATAFAKRGLIAEHGIAWILPR
                     IVGQAHALDLLLSSRKIGAEEALRMNLVGRLCEPAALMPQALAYARDLATLVSPRSLC
                     VMKRQVYEAPFQGLAEATVVANREMYDSISSDDFKEGVSHFLDHRPPRFTGA"
     gene            complement(15423..16886)
                     /locus_tag="VGC69_13160"
     CDS             complement(15423..16886)
                     /locus_tag="VGC69_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0296272.1"
                     /translation="MVEHSSVYSMAGEGHRERYWQNYVDGAWKDCQGGAATTVLDSYT
                     EAPMAEVGAASGGDVDAAVAAAREAFDSWSTSPLAQRQALVHAIGDRLAAEAQSLAAQ
                     ITREVGMPLNLSSIIQVDMPVKAWRRYAELAGTVEWQKRIGHSTVSQAPIGVVACITP
                     WNYPLHQITSKVAPALVAGCSVVLKPSELAPASAFVLARAAEAVGLPAGVFNLVWGDG
                     AGTGRALISHPDVDMVSFTGSTRVGEQVGSIAGGQIKRLALELGGKSACIALPDADPA
                     AVARHALSACMLNSGQSCNAISRLLVPRAMLEVFRTRLAEGIGKYVMGDPMDRATRLG
                     PLVSRQQQARVRGMAGRAMDDGCEVFAGNAGMALPPTGFFVPPLVLLANGSEPVAREE
                     VFGPVLCVIPYDDEEQAVAIANDTDYGLAGAVWSAQPQRAFEVARRMRAGQVDVNGAA
                     FNPLAPFGGFRKSGLGREGGLFGIEEFLEPHSIQMPD"
     gene            complement(16900..17877)
                     /locus_tag="VGC69_13165"
     CDS             complement(16900..17877)
                     /locus_tag="VGC69_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296273.1"
                     /translation="MKALKIFPAAVVAALATALLPVSASRANDSYPDKPIRLVVPFTP
                     GGASDNLSRGLAIEMGKRMGQSVIIVNKPGAGTVIASEFVANSPADGYTLLWIAPPFA
                     INATLMKHLPYDTRRAFTSVCDIASSPMLVVVNKDSHIKTLDQLVDRAKHKPLSFGTS
                     GIGGTPHLATVMFENATGMKMTHVPYQGSAPAVTALLGGQVDLVIDTILTTLPQVSAG
                     KLVALAQTGRTRSSLVPDLPTMQELGIKGYEANTWFSVLAPAGTPKPIVDKLNSVMNE
                     ILRDSQFRATFANLGLVMMGGSVQQANERLMGEIERWGEAVRQSGASVD"
     gene            complement(17912..18652)
                     /locus_tag="VGC69_13170"
     CDS             complement(17912..18652)
                     /locus_tag="VGC69_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0296274.1"
                     /translation="MPHIQIDNRSSGVTVITLDRPEKRNAINAAMALELQDAFAHFDQ
                     SSQRVAVLTGQGESAFSAGADVSDMPEFWRCVPTLGIQTDKPIIMALSGWCVGGALVI
                     AMMADLAVASETTRFLYPEARLGLTGGMIAGLAARIPHKVAMEVMLLGRPLTARRAHE
                     VGLVNEVVPAGTHLAAAIAMAEELAGYAPMVLAALKRFVNQEMLAASPSEQASRTMQV
                     LRAIETSADKAEGVAAFLEKRKPRYMGK"
     gene            complement(18695..20836)
                     /locus_tag="VGC69_13175"
     CDS             complement(18695..20836)
                     /locus_tag="VGC69_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0296275.1"
                     /translation="MSDNHASASSRPLAHLLQPSSIAVIGASEDQTKFGGRLLRMLLR
                     HRFAGQIYPVNPGRKQLFGLQTWSNLGDLPQAPDMAVLTIPQPAIKAQIETAARMGVR
                     CCVIITSGFSDASAAGAQAEAEIVEIARTHGMRLIGPNCLGIISAVNRLALCSSPVLE
                     IAALPQQPIGFVSQSGALMTTFFDRAWSQGIGFSHGVSVGNQADLELCDFVEFLADDP
                     DTRVICTYIEGVKDAARFVQAARRVKASGKPWLAVKAGRTEAGSRAAFSHTASIAGDY
                     AVFAAVCREEGVTLMSDVGAMVTLAAMLARYGERRVDRLAILTPSGGGGALASDALSE
                     RGVKLAEFSEQTRALLAQHYPEGQANNPVDFGSRKTVDPVCAANATLQALETDARTDG
                     VMIPVTMAPPAWLQAFARAQPEAGAAQGKPVLFTLEAGHASDELRELLSERKLPYTNH
                     IGEAAHVWETWRDLSTRPGVQEQPANRPKGAGPERSLSPGDYGEDDSKACFARYGIPV
                     NQGEIAASADQAVEIAERMGYPVVMKVVSPDIVHKTEAGAVAVGVADTSSVRERYDGL
                     IGNAARAVPSARLLGVSVQTMVRGELELIVGARRDPQFGPIVVFGAGGILVELLRSMA
                     VARAPTHPDHVLALLRTLPIWPVLQGYRGSPLAIDAAVDAIVRVSWLAHDLAGSEFEC
                     DINPLIVGRRECWAVDARLRIAQQCRPRDRT"
     gene            21259..22638
                     /locus_tag="VGC69_13180"
     CDS             21259..22638
                     /locus_tag="VGC69_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296276.1"
                     /translation="MTHPTPNLSPIPLTRQLARQALAFDARDIPEDVKEIARHCLLDW
                     FSVALAAWDSPALRALEAWAMLEPSPGSASLLGSAQRVSTSQAALINGTASHILDFDD
                     AHLPSRVHPSAPLWPAILALAQTRNLSGERVLAAFAAGVQVQSRLGAVMGNSHYREGW
                     HNTGTLGTFGATAAVGRLEGLEEEALCQAFGFAATLTGGLRSVFGSSAKPLHAGRAAA
                     NGLMAAGLVQQGMQTVADILERPGGFVQTYARDFDTAQIESPAGLWEIRRIVFKYHAS
                     CYGTQAPIEAALALRKHADRASTCRVDVHVEPQYMTVCNIAKPSSVSEAKFSIAHMVA
                     LALAGRDTSNEKNLSGSALHDPDILAWRDLIHVHGDPAMARANARVVVHGPGGEAAHT
                     HNASQPQADLAQQRRRLLEKSSALLRPRYGERASTLAQERLLAIDRQSELADWLHQLL
                     AELNQASRA"
     gene            complement(22661..23509)
                     /locus_tag="VGC69_13185"
     CDS             complement(22661..23509)
                     /locus_tag="VGC69_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0296277.1"
                     /translation="MSCILCGMPVNPLRSGSRPRGLERQRIALDMDKTFLKGLTLLET
                     LARSERACGVTELADTLQLTKSNVHRLLQGLVYQGFARKTGDTGRYELTMKLWELGSH
                     VLSRLDVRQVAQPFLERLAADTSETVHLSVLDGIDVLYVAKIDSPQPVRSYTTVGGRA
                     PAQCVATGKAQLAWADANRIAQVKAALRAYSSKSITRAADLDAELERIRSQGYAINRG
                     EWREQVCGVAAAIRDASGEVIAAVGLSGPADRLKARQMKEWGPPVAEIAGQISRLLGY
                     TAAPTR"
     gene            23508..23738
                     /locus_tag="VGC69_13190"
     CDS             23508..23738
                     /locus_tag="VGC69_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296278.1"
                     /translation="MAATAAGPVHLIVTRNYTEFQKMERRVTSCLRKSLDTPNHAALR
                     FPRYFQNNVLYIGTTEDHRIHLMPETTCQLQN"
     gene            23768..24289
                     /locus_tag="VGC69_13195"
     CDS             23768..24289
                     /locus_tag="VGC69_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HEY0296279.1"
                     /translation="MDSVADDRARMLATLPILERLVASDDWLPEVFAQPHPEHYQQYL
                     LYCDPHERFSIVSFVWGPGQQTPVHDHTVWGLVGMLRGSETSQRFEMRNGALREGGLA
                     TLEPGQIERLLPKEGDIHQVCNRYDDRVSISIHVYGANIGRVRRHVFDPKTSQSKEFV
                     SGFSSAMLPNIWN"
     gene            24322..25686
                     /gene="purB"
                     /locus_tag="VGC69_13200"
     CDS             24322..25686
                     /gene="purB"
                     /locus_tag="VGC69_13200"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY0296280.1"
                     /translation="MGSYVIDSELFRDQFGTEAMRDIFSDRTTVQRWLDVEAALAKVQ
                     ARMSIIPQAAADEIQRKAKVGLIDLQELKAEMDRTAHPIVPLLRAMKKICADDAGEYI
                     HWGATTQDIMDTGTILQLRDALREIDKSLRAVLDKAIVLARRHRDSTMVARSHGQQAL
                     PITFGFKVAVWVAEIRRNLARLAQMRERVLVGQFSGAVGTLAALGEQGIEVQEALFQE
                     LGLRTPEITWHVSRDGTAELACVLGICVGTLGKIAHEIYSLQKTETAELEEPFSPGKV
                     GSSTMPHKRNPPTCETVVAISRIVRSTVPLALETMIAEHERDKIALQTEREFVSRMCS
                     MTHAAAQKMTFVLDKLSVRTDNMEKNLYIQNGLLLSEAVMMHLSDALGRQEAHDIVYR
                     VCMDVFERGGNLKDAILSDPQLKGRISAQDLDRVLDPHAYTGLAGVFVDRVLSRPAPA
                     APGD"
     gene            complement(25755..26096)
                     /locus_tag="VGC69_13205"
     CDS             complement(25755..26096)
                     /locus_tag="VGC69_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0296281.1"
                     /translation="MLNNWVEFLSKVRSAARLLASANPPVVAAYRGLNEAQAGGKALD
                     AKTRELISLAVAVTTRCDGCIASHVEAARKAGVTQEELGEALGTAIALNAGAAYIYSV
                     RAMEAFDQFSK"
     gene            26372..27580
                     /locus_tag="VGC69_13210"
     CDS             26372..27580
                     /locus_tag="VGC69_13210"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0296282.1"
                     /translation="MTMQTQDDSENSAHPPLRGVRVIEFCQIAAGPFCGMLLADFGAD
                     VTKVEPPDGDGLRQWPPISDGFSENFASLNRGKRSVCLDLKNPAQRDLARALVLDADV
                     LVENSRPGVMARLGLGWDWFGPRKPGLVYCSISAYGQTGPRRAEGGFDLTIQAAAGVM
                     SVTGEPGGEPVKCGVPVSDFTAGLYAAFTISAMLARVRAGGPGGHVDVPMFATTLAIA
                     ALQTSEYFGTGRNPRKLGSAHPRNAPYQAYQASDGWFAIAAGNNRLWEAVCAVVGGEA
                     WRNDPRFVSNAQRAAHQAELKALLDPLFARRKVSDWLDDFRAAGVPCAPINDYASALD
                     DPQARHLQLTQPMALPGGHATRTVGCPVWLDGRPVEVQTIPPGLGQHTNDARSRYAFD
                     IHAAGTREDR"
     gene            27577..28344
                     /locus_tag="VGC69_13215"
     CDS             27577..28344
                     /locus_tag="VGC69_13215"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0296283.1"
                     /translation="MIPPDNAGAPACLEIRAAVATLWLNRVQQANALSADLVEALIAH
                     TTTAFADTALHTLVLRSAGRHFCSGFDMSDLEQQSDGDLLWRFVRIETLLDTLWNAPV
                     RTIAVADSRAWGAGADLFAACDLRIALPQANFAFPGSRFGLVLGTRRLAARVGREPAV
                     EIASAARILDAREAHRCGLASHLCDAEALDDLLAQLAGTGTAQRDVGEALRCAARAGE
                     AALRDSDLAALVRSATRSGLKQRLADYRAAVRRSAGR"
     gene            complement(28464..29480)
                     /locus_tag="VGC69_13220"
     CDS             complement(28464..29480)
                     /locus_tag="VGC69_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY0296284.1"
                     /translation="MKNLDEATERICELKGSLVAMDALLPALLEALPSSVHDALTRSF
                     EAHAEAARTVMLNADISDHVLAAFERDIARTQAVLAGIVSADSPPDPGNAAEAVLLTA
                     TRVSTFLGRQPLSGASGFFFRRDQRLFLVTNRHVFVDEASGHCPDRIEIEVHIDARDL
                     TRYATFSIPLYRGGRGLWRQAADSAGAVDIAVIEIDADCLPEDVMLQAFDPSNLDPRG
                     EEVAVGDALTVIGFPLGFHDTIHHLAVARSASIASAYGVRFQQQGYFLTDARTHRGSS
                     GSPVLRRRGEHAGDDSPLRWQLLGVHSTRMDMRTRDQVEDESLGLNCAWYADVLMVLT
                     VSQK"
     gene            complement(29573..29785)
                     /locus_tag="VGC69_13225"
     CDS             complement(29573..29785)
                     /locus_tag="VGC69_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0296285.1"
                     /translation="MTTQIGTVKWFNEGKGFGFIAPDDGGKDLFAHFREIQSGAFKTL
                     TENQRVQFEVTQGEKGPQASNIRAVS"
     gene            complement(30063..31109)
                     /locus_tag="VGC69_13230"
     CDS             complement(30063..31109)
                     /locus_tag="VGC69_13230"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0296286.1"
                     /translation="MIQVRGYATHDAQSPLVPFQFEHREPGPNDVQIKILYAGICHSD
                     LHQARNDWSNPRYPMVPGHEIVGEVVAVGDEVGKFKVGDLAGVGCMVESCRICSACRL
                     DLEQYCEQGATLTYNDVERGTDKLTFGGYSEQVVVDEHFVAKISPKLDLKAVAPLLCA
                     GITTYSPLRHWKVGLGQKVGVIGLGGLGHMGIKFAKAMGAHVVMITTSPDKGRDATRL
                     GADEVLLSRDADAMAKHANSFDFLLNTIPVGHDVNPYLSLLKLDKTMALVGALTALTP
                     PPTGISVIHGRKSLAGSAIGGMAETQEMLDFCAESGIVSDIEMIDMAYVNTAYERMLK
                     NDVKYRFVIDIATL"
     gene            31225..32112
                     /locus_tag="VGC69_13235"
     CDS             31225..32112
                     /locus_tag="VGC69_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296287.1"
                     /translation="MQADNLAHLAAFAAVARHHSFRKASAELALSPSAVSYAIRSLEE
                     RLGVNLFNRTTRSVSLTAAGQRLLERIQPALRDVGHALDEMNAFRSTPTGTLRINTSR
                     ASAYLLVAPLVPRFLAAYPDIRLEIVDDDSFVDIVAEGYDVGIRFQEAVPEDMVAVPI
                     AKAQRFALVGSPAYFARHAIPRHPTDLLGHDCVRHRFPSGRIYKWEFERDGAKLEIDP
                     PGRVTLGDLHLMTRMALDGVGLTLQLEEHVRDLVESGRLLRSMEDWCPPFPGFMLYYP
                     RQQRMSSALRAFIDMVRET"
     gene            complement(32120..32917)
                     /gene="eutC"
                     /locus_tag="VGC69_13240"
     CDS             complement(32120..32917)
                     /gene="eutC"
                     /locus_tag="VGC69_13240"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEY0296288.1"
                     /translation="MSPSPDSLVQDSPWDLLRRHTSARIALGRVGASLPSAEILKFGL
                     AHARARDAVHHPLDADALEAQLRERGLRVARVRSQAGDRHTYLLRPDLGRRLAPADGQ
                     ALRAAAPAPELAIVMADGLSAVAVQRHAVPLLDALRRCYDTDWARTPIVLAEQGRVAL
                     GDDIGEALRARMVIVFIGERPGLTAPDSLGIYFTYAPRTGRLDSERNCISNVRPEGLP
                     IDAAAGKLDYLVRQALRRKLTGVGLKDDSEALAPPVPPASPDPAGEG"
     gene            complement(32925..34316)
                     /locus_tag="VGC69_13245"
     CDS             complement(32925..34316)
                     /locus_tag="VGC69_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY0296289.1"
                     /translation="MAYHTRFQALGYQFDGLKTLLAKASPARSGDQLAGLAAASYEER
                     MAARIALADVPLRDFLNDALIPYEQDEITRLIIDTHDQAAFAPIAHLTVGEFRNWLLL
                     ESTDSAALACAAPGITPEMAAAASKLMRNQDLILAAKKCQVVTRFRDTIGLPGHLAVR
                     LQPNHPTDDIAGIAASMLDGLLYGAGDAVVGVNPAGDSPAALMALWRMLDEVRAHFDI
                     PTQTCVLTHVTTQIAAIEQGAPVDLVFQSIAGTEQANQGFGISLALLREAREAALSLK
                     RGTVGDNVMYFETGQGSALSAGAHHGVDQQTCEARAYAVARHFKPLLTNTVVGFIGPE
                     YLYDGKQIIRAGLEDHFCGKLLGVPMGCDICYTNHAEADQDDMDTLLTLLGAAGINFI
                     MGVPGADDIMLNYQSTSFHDALYLREVLGKRRAPEFQAWLERMAIVDAGGRLLDVGAG
                     NALIRQLPRLAAS"
     gene            34389..>34760
                     /locus_tag="VGC69_13250"
     CDS             34389..>34760
                     /locus_tag="VGC69_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0296290.1"
                     /translation="MPRSASTKNPSEAAAEKLLAFRVLSLGQLLNRGIAVLLDRHLGL
                     SVRQWRVLFYLANSGPDSPQNIAAFCRYDKSQVSRAIAELQGKKLARTSASATDGRRL
                     EVSLTAAGRAAYARGLPVSQAR"
BASE COUNT         6579 a        11326 c        10660 g         6195 t
ORIGIN      
        1 cccggcgccg cgacgcgatg cggcgcatgt ccttggcgcg caaggtatcc agcgccaagg
       61 acggcggcac cgcatgcatc tcgtcgcgca gcgcctgcag gcgctgccag gccgcgcgat
      121 gcagcggccc ggcctgcagc cagtgttcga attcctgccg cgtggccgcg tcgggttcgt
      181 tgtagttcag cttgacggcc caggcgacgg ccgcgtcgac cacccgcgcc gggatgtcct
      241 cggcgccgcc gccaaggacg gcgcgttcag tactcgaagc gcagtgcata gcaatgccgc
      301 aaggcggttg cgacatagcg ttcggcggta gccagcgaaa cccccaggcg gcgggcgatc
      361 tcagggcagc tggcgccttc cagctgcgcc cacaggaagg cgctgcggac ggccggcttg
      421 agctgcgaca gcatggcgtc gatgcggtcc agcgcctcga gcaggatcag gcgcacctcg
      481 ggcgaaggca cttgcggttc gggcaactgg gccagcgtgg ccagccaggc atgctccagg
      541 tcgcgccggc gccaatgttc caccagcagc ccatgggcaa tggtggtgag ataggcgcgg
      601 ggagatttca ggcccttctg caagtcttcc tggcgcttga gcagcacacg caagaaggtg
      661 tcctgcgcca ggtcggccgc ctggtggctg caacccagct tgccccgcag ccacgcgacc
      721 agccagccgt ggtgatcggc gtagagggct tcgatcggat tgacggaaga gatcatggag
      781 aaagtccgca tcgccgcacc cgcgacgtgc cgcgatgcgg cgtattgaga tctgtaattt
      841 gtaatgcgaa ttagtctcaa ttctagagtc gagcaggcct tcctgcaagc cggccgcgat
      901 gcgcgggacg gaccgcgcaa ggccgtcgac ttgctcccct ttttagaata cggtatagtt
      961 attgactctc ttctttcgca gcaattttgc gaacgacgat gcgggagtag ctcagttggt
     1021 agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtctccc gctccaagga
     1081 ttttgttcca tggacgtcta tagaaatcca tagaacacag agaaaaggag cttcggctcc
     1141 ttttttcgtt tctggagcgt ccatcaacat ccagggacgt ccacctacag ccagcgtttt
     1201 ttaggctcta tttaaggcca taattgatcc ggcgccgagg cacttacaca ggcgacgctg
     1261 gctgagaggg ctacgattca agttcctgca ctgcaagagg agcatgaacg ccatgccact
     1321 cacagacatc gcagtccggc aggcccgccc cgcggacaag gcctacaccc ttagtgatgg
     1381 tcgcggactg ggtttgtgcg tgacgcccag cgggggcaaa cactggcatt tccgctagtc
     1441 gtggtcaggc aagcagcatc gtatgacgtt gggggcctac cccgagatca gcctgaaaga
     1501 ggctcgcgag ctgcgagaag cggctcgggc tcaggtcgca cgaggtatcc accctcggaa
     1561 gcaccgcaag caagaactgc tggtgaaacg cttggccgaa gatcgacggg tccaaggggt
     1621 cttcgaccaa tggctctcac accgcagccg cacgctgcaa cctggccgcc aaagtacgct
     1681 cggtcaaatt gaacgaattt tccgcaggga tattctccct accctggcca aacgctcgat
     1741 ctacgaaatt acccaggccg atctgctgca agtactggga aaaatcgagg cacgcgaggc
     1801 actcaccact gccgaaaaat gccgctcatg gctcaggcag ctcttccggt tcgcgatggt
     1861 caaagtcccc gggttggaga aaaacccggc gcaagatctg gctgtagcgg cgctacccaa
     1921 gccgccggtc gcgcataacc ccttcctgca gattgcagac ttgccagact tgcttcaggc
     1981 attacgcagt tatcaaggta gcccggcagt acaaatggca ctgcgcctgc tgctcttgac
     2041 cggtgttcga accggcgagc taaggcgcgc aacacgggac cagttcgatc tggatcgagg
     2101 gttgtggacc attccagcag tgcacgtcaa acagttgcac gccaagatgc acaagaaagg
     2161 gctgggccca caagatattc cgccttattt gatccctctg cctacgcagg cacccaaaga
     2221 ggccgaggcc gtttcaaacg tggtcaatgt ggaaaccggc accacagtga cgatcaccat
     2281 ttctacgggc caagctccga acaggacgca gcgtgatgct ctcatgacga gcccatccac
     2341 gaaggtgtca gacaacgagc aactaagcct ctacgaagcg ccgggaaacc tgccgctgtc
     2401 tatgtttgct agcttggcag agaaacctga ggaacgtaac cgacactaga tggcccacag
     2461 cttcaagcac tggctacgga acacaccgat gcggggcccg cgctcgaagg caggggacga
     2521 atcaagccat tcgaacctat tgaacgaatc gaacgattga ggtaagatgg ctcgtagact
     2581 tgcacgagga gtccgaaatg caaaccttta ctgccaatga ggcaaaaact cgattcggtg
     2641 aactgatcga ccgggtgcaa cgagagccga tccaggtcac gcggcgcaac cgggttgtcg
     2701 gcgtcatggt gtcggcggag gactaccagg cgatgcgtga gttctatgca gatcggctac
     2761 gacgcacctt gaagcaaagt gccgaagaag ccgccggcaa gggcttgacg gacgagaagc
     2821 tggaacagct cctggccaat gaggattgac cgcgctgtca tcgacaccaa cgtcctgata
     2881 agtgctgcgc tatcgcctcg gtctgcccct gcgcagatcg tggattgtct tctgcaacac
     2941 gctacgttgg tgttctcacg ggaaaccttc gaggaacttg aaaccaggct ctggcggcca
     3001 aaatttgacc gctatctgga catggatcgc cgacgcctgc tgttgcatga cttcgctgcg
     3061 gcggctcggt gggttgatct gcctccgact cccagaccgg agtacagccg cgatcccgat
     3121 gacgatgtgt ttctccacac ggccctgcat ggcaaggccg aatggctagt cagtggcgac
     3181 aaggatttgc tggagcttcc accggctgac tggggattcg aaatcctgtc gccagcgcag
     3241 gctctagaga aatggcatca aaaatgagca tcttggaaaa atggccagtc gaacaaatat
     3301 tgaacactcg catacgctta cccaggcatt cctcgctttg ctagaccgag atatccaagc
     3361 tggcgtcaat gtccggccac ttccggagga tttggctcag ttaatgctgg cacaaactca
     3421 gcttcgcccc gatgttgatc tggatgaaga gttcgaaggt ggcattgacc tttgacaagt
     3481 tctgccttca acaagaatgg ccatgcctcg gatacgcgta ctgacctgtg cctccagcac
     3541 cgacgcctat gttccgtgga ggtacatgac caatcatgga tggcaatgga acaacttgtt
     3601 gtcgaagatg aaaaacaagg ctaaagttga ggcgcctgcg actcacagcg gccaagaaag
     3661 catttggaaa acaaagactt agcgctcaag tgcaagtctc gtttcccgct ccaggattta
     3721 gcctgcgggc gtctactgaa agtctgcaag gtgttggtta aggggcgaat tgaaaaattc
     3781 gccccttttt cattgccatg cagcacattc agactatgag gatgcgtatc gggttccacg
     3841 gtggcccagt gccggtcccg cgcagcctgg cgtttgccaa ccttgcccgc aaccctatag
     3901 aataaaaacc attctcattt gatatgggtt tcctgctgcc ctgggccggg gaaagggcat
     3961 cgtggccata tccgaattca cgcctgagcg gcaaatcgaa gcgttgtacg ccgaccatca
     4021 cggctggttg ctgggatggt tgcgccgcag gctgggcggc ggcgtccacg acgccgccga
     4081 tgtaacccag gataccttcg tccgcatcct ggcatcccgg gatgccctgc tgggcgtcaa
     4141 ggaaccgcgc gcctacctca ccaccatcgc gacgcgatta ctggtggacc gttcccgcag
     4201 ggagttggtg gaacggacct acctggcgga aatggccttg ctggtggaaa acccgcccag
     4261 ctatcctgct cccgatgatg tcctgatggc cgtgcaggct ctggaacaga tcggcgccgc
     4321 gctggaaggc gtgcccccca aggtgcgcga ggcgttcttg cggcactatc tggacgagca
     4381 aacgcatgcg gccattgccg ccgatctggg cgtgtccaaa cgcatggtcc aaaaatacct
     4441 ggtccaggcg ctgctgcatt gccgcgcccg gtgtccggcg atggccgaac atggggcaaa
     4501 gcccgccggt gcgaaagacg tttcatgacc cagcctgaat cccgtcccga cttgccggat
     4561 gagaccgatc tgctcaccga gcaagcggcg cagtggatcg tgctgctgac cgacgatgat
     4621 gaggccacgc gcacccaggc tcgcgcgggg ttcgagagat ggaagcaggc agacacccgc
     4681 catgccgccg cggcggcaag tctggagcag ctcatcggcc aggtccgcgg cgtgcgcgaa
     4741 accgcgggcg gcagcgctcg cccggcacgg gcggcgctcg atgccgtctt cgccgaccgc
     4801 cgccatcgcc agcggctcaa gcgcgccggc gccgcgctgc tggcagtctt cgtgctggcc
     4861 gccccggcat ggatggcgct gcgcgcctgg ccgcccgcct atgcgatggc cgacatccgc
     4921 acggccactg gccaatggga aacccgcctg ctgtccgacg gcacccgcat cacgctcgat
     4981 agcgccagcg ccgtcgatct gcactacgac gcccaccggc gcgccattga acttgtgcag
     5041 ggcgagatcc tggtggacgt ggccaaggat gcggcgcgtc ctttcctggt cgaaaccacg
     5101 catggcagca ttcgcgcgct gggcacccgc tttgtggtcg gccaccagga cgatgctacc
     5161 gtgctcagca tgatcgaatc gcgcgttgcg gtacagactg cccggcagcg cgccgccggc
     5221 agcagcgaga cgacggaagt cgttgccggc caacgcgtgc gcattaccga gaacgccgtg
     5281 ggtccggctg aacccatcga cacgcgcagc ctcgcggatg cctggaaata tcaccagctc
     5341 gtcgtcgata accggccgtt gcccgaggtg ctggatgaac tggcgcacta tcggccggga
     5401 catatccact atgacctggc ggagatcgaa ccaatccggg tcagcgcggt gctgcccctg
     5461 gacgaccccg accaggcatt gcagttgttg catatcaagt tcccggcttt gcgcatacgc
     5521 accgtaacgc cctggttggt gctggtggac gctccctcgc ccaagtagat ttcgcggatt
     5581 gcaaaaaaat cgatcgccgg cagttccact ttgtcgttct catccgtcaa acctgatgag
     5641 aacgaaaagc aaccgcatgg aaggcggcgc gagccaagcc tgttccggaa gttccttttc
     5701 gtgcatcgcc cgtcgtgggc agagaagatc aacgaaccca ggaaccgcca accatgtccg
     5761 tgtcgcccgt ttcggcacaa gtgtccgccg aaccctccct ccgtgcccgc ccgacgccgc
     5821 tcgcgctggc cgtgcatgcc gcctttgcca cggcgctggc gctgggtttt atccaatgca
     5881 gccaagccca aacgccagcg caggctgccc agtacgacgt gcccgccggg ccattggccg
     5941 aagccgtgaa ccgcttcgcg cagcagtccg gggtgtcgat cgtgatggat gccaaccaga
     6001 tccgggggct gacctcgccg ggcctgaggg gcaattatgg cgtccaggaa ggtttcgata
     6061 tgttgttgcg cggcagtgga tataccgccg gaaaaacgcc ggccggctat gtgctgcgtc
     6121 ccacgccgcc gtcgacttcc ggcatgagag gagaaagtcc ggccgtcatg ccggcggttt
     6181 cggtcgtcgg agcaaggccc tcagacgagt tgccgcccgc ctatgccggc gggcaagtgg
     6241 ccacaggcgg acgcgtcggc ctgcttggca accgggacat gatggatacg cccttcagcg
     6301 ttgtcagcta caccagcaaa accattgaag accatcaggc ccagagcatc ggcgatgcgc
     6361 tgatgaacga cccttcggta agggtggaca ggcctgccgg cagctatgcc gatgaagtct
     6421 acattcgagg cttcccgctg atgaatcccg acatattctt caacgggctg tccggcgtac
     6481 tgccctataa cttcgtcggg ccggaattcc tggaacgcgt ggaactgctc aggggcgcca
     6541 gcggcctgtt gtatggcatt tcgccgaacg gcgccgtcgg aggcgccatc aatgtcgtcc
     6601 ccaagcgcgc caccgatgat cccatcaccc ggctgacgac gacctacagt tcggattccc
     6661 agttcggcac gcatgttgac gtgggacgcc gatttggcga agacaataga tggggcatcc
     6721 ggttcaatgg aacgttccgg gatggcgata tgtcggccaa caatacctcg gaacgcctcg
     6781 gggccggaat gctgggcctg gactatcggg gcgacaggtt tcgattttcc ctggatggcg
     6841 gctaccagaa ccggaggctg aaagggttct tcgacagcct cgatttcctg tccaatgact
     6901 tcaaggtccc atccgcgccc aaggccagcg acaactaccc ttcctggacc cggttcaact
     6961 cggaagacac attcgggatg gcgagggccg agtatgacat tgcaccggac tggacggtct
     7021 ttgcggcggc gggcttctcg cacaacaact cctccacatt gggaggagta ggactgctgg
     7081 aaaattcagc gggcgatgcg ctgttttttc cgcgcgccaa caaattcacc gaggacagcg
     7141 cttccaccca agtcgggatc agggggaaat tcgacaccgg gccgatcggc catcaagtgt
     7201 cgcttaccgg ggactattac cgccaccgag ccgctttcgg gcagactctt tttgcttcgc
     7261 aggacgtgaa tatctatgat cccggcagct tctcggcacc ggatctttct tcctactcac
     7321 cgaatgcgcc gctgtcatcc atcacagaga actcgtccgt cgccgccgcg gacacgctgt
     7381 cgatcctcga cgatcgcatc cagttgacgg caggtctgcg ggaacagcac atcgacaccc
     7441 gaaactatgc cgcgacggga acggtgtcgt ccgaatatga ccgaagcgtc ctcacgccgg
     7501 ccgtcgcatt cgttgtcaaa ccctgggagc gggtttctct ctatgcaaac tatatcgaag
     7561 ggctgcaacc cggcgccgtc gtgcccgcca cctacgccaa cggcggccag gccctgccgc
     7621 cttatgtctc tcgtcaatac gagataggaa cgaaggttga ttggggcaag gtgaccacga
     7681 cgttatcgct gttccagatc aagcagccga gcggctccgc agacccgttg accaatatct
     7741 actccgcgaa cggcgaacaa cgaaaccgag gcatcgaact gcaggccttt ggcgaagcgg
     7801 cgcaaggggt tcggctgttg ggaggcatcg cactgacgga cgcccggctt acccagacaa
     7861 actctcccgc cacgcaagga aacaggccat acggtgtgcc ggacgtgcaa gcgaatctag
     7921 gggttgagtg ggataccccg tttgtccaga atcttacgct gactggaaat gcggtctata
     7981 catcgtcgca gtacatcgac gcaacgaacc agcagaaaat cccaagctgg atccgcttcg
     8041 atgtaggcgc ccggtacgtc tggcaacgcg aaaatggcaa acccatcacg ttccgggcgc
     8101 aaattctcaa tgtcaccaat cggcggtatt ggtcgtccta ttcgtttacg aacagccttg
     8161 ccgaaggggc ccctcgcacc gttatgctgt cggctcaatt cgacttctag aacgtaaggc
     8221 gtgccggcag actggcggcg gcggggaatg tcgttgccag gccacacatt gccatggctt
     8281 tcagcgcttg aactggcggc acgcctacgt gccgattgcg gaattgcgga tttctgataa
     8341 taattaagat taattcttat ttagattcac gaatggcgtc ccaggatagg ccgacgccac
     8401 tggaacgcac cgtgccgaac gaggcaaccc ccgcggaatt cgagtccctg tatggcagcc
     8461 atcatggatg gttgcaggga tggctgcacc gccgcctggg caacgcggcc gatgcggcgg
     8521 accttgctca cgacgccttc ttgcggctga tgcagacgcc gcgccgcttc gacagcgcgc
     8581 cgcaggcccg cagctacctg cgcacgatgg ccaacggcct gtgcgtggat ctctggcgcc
     8641 gacgcagcat cgaacaagcc tggctcgacg cgctcgccgc ccagtccgag gccgtggcgc
     8701 cttcggccga gcatcaggcc atcgtgctcg aagccctcca ggaaatcgac gccatgctgc
     8761 acagcctgcc gcccaaggcc gcccaggcct tcgtgctggc cgtggccggc ggcatgaccc
     8821 acaaggaagt ggcccgggaa ctgggcgtct ccacacgcac ggtgacgacc tacatcaccc
     8881 aggccatgct gcactgcctg catctcgaag cgcggcaagt ggtgcaagag ttgccgtacg
     8941 gcgcccgcat gccagcgtcc gcctccccca ttcccagcgc cttatgatgg cgagccggcc
     9001 cctccggtaa tcgcgccatg gccaccgcct ccgtcggcgc cccctcttgc gtgccgtcgc
     9061 accgggcact tcagcaggca gccgaatggt tcgcgctgct ggattcgggc gaggcaacag
     9121 ccaccgaccg cggccgctgg gatgcctggc tggccgaatc ggctgaacat cgggaggcat
     9181 ggcgcttcgt cgagcgcatc agccgccgct tcgatcccat caaggccagc ccggagcggc
     9241 gcaccgcgct cactgcttac cagttggccc atggaatacg gatacggcgc cggcagaccc
     9301 tgttgagcct ggcggccctg accggcagcg gcctgctcgg ctggaccgtg tggcgccagg
     9361 cttcggtgcc cgaactggcc ctgtcgtggg tggccgacta ccgtaccggc acgggcgaga
     9421 tacacggcat cgtgctggcc gacggcaccc gcgtctggct caacgccgca agcgccttca
     9481 atcaggacta ccagccggat cagcgcaagc tgcgcctggt caggggagaa atcctcgtcg
     9541 acaccgccgc cgatccattg ggtcggcctt tctacgtcga gacgtcccaa ggccaaatgc
     9601 aggcgttggg cacccggttc accgtgcgcc tggacggaag cgaaaccttc ctggccgtct
     9661 acgaaggttc tgtcgaggtg cgcaccgccg acaccggcgc catggccgtc attcccgcag
     9721 gccggcagat ccgcttcacc cgcgcggccc tggcggcctc cgaaaccgcc gatccggccc
     9781 gtgaggcctg gacccgcggg ctgctcgtcg cccgcgatat cccgctggcc gaggtagtgc
     9841 aggaactgcg gcgctattat cccggccact tgagcctggc gcccgaagtg gccgacctgc
     9901 gggttttagg cggctacccg gtcaacgacc ccgacaaggt cctggccatg ctcgaatccg
     9961 tcttgcccat ccgcgtgcaa cgcacgctgc cgtggtgggt gagcatcgag cctcgccaag
    10021 aagccgcttc cagccgctga ccccgcatcg gccacccgca ccgatccagc ccgacctttt
    10081 atcgaaagcg gcttgcgccg gatgtcgtgg gtgagtgacg agtttttttc aaaacggctt
    10141 ccaccttttg gcctctcctt cgattaaggg ggtgagagat caccaagtca cccccaaccg
    10201 gcctcccgga cctgtcattg gccggttcgg ccgcacccgg cagcaaggtg gtctgactcc
    10261 atttcgtcaa ccgctctgca cgaggaagtc cgttcgccat gtctcgccag tcatcccatg
    10321 ccgtttccgc cgccgcggcc atcgtttccg gtgctgtccc gcgaaccaat gcgaggctcc
    10381 gccccaccgt gcttgcagcc gccatgcatc tggcgctggc gggcgggcta ctggccgcgc
    10441 ggcaacccgc ggctcacgct caagccgcgg cggcccaacc gtcgcaagca ctggctgcgc
    10501 gagcctacga catcccctcg gggccgctcg aagcggcgtt gacgcgattc gtcaccgagt
    10561 ccggcatgcc gctggccgcc acgcccggac tggtccaggg caagcaaagc ccaggcttgc
    10621 acggcacctt cggcgcacag gccgcattga atgccctgct ggctggaacg gggctggagg
    10681 ctgtcgtcgg caacgagggc tcctacgtgt tgcgccaggc tcccgcggag acctcgggcg
    10741 ctggcattgc aaccctgcct gccattgcgg taacgggcgt ggcgactgtt gatctgcctc
    10801 ctgtctacgc aggtggacag gttgcaagtg gcggccagct tggattcctt ggaaatcgaa
    10861 gcatattcga taccccgttc aatcagtcaa gctatacgag ccagctcatt caagatcaac
    10921 aagcacggag tatcgccgat gttgcggcaa acgatccgtc tgttagacag gcatgggtaa
    10981 gcggcagtta tacggatcaa ttttatattc gaggcttccc tgtcggcgca tccgacgtcg
    11041 ccgttaacgg catctttggt cttgcaccgt ttcaatattc caatacagat tgggtcgatc
    11101 gcgtcgaaat tctgaaaggt actaacgcat tgcttaacgg gatgtcaccg ggaggcagcg
    11161 tagggggcaa tatcaatctg gtcccgaagc aggccgaaga cacaccgatc acgcaactta
    11221 caacgtccta tatgtcgaga ggtcaactcg gcacaaacgt ggacattggg cggcgctttg
    11281 ggacaaatca agagtgggga attcgcttcg acggcaccta caaaaacggc gacaccagtc
    11341 gggatagcca gcgccaggag cttgggtcgg cctttctcgg acttgactat cgcggagagc
    11401 gctttcgcat gtctctcgat gctggctacc agcatcaaga cttcgcgtca ccaacacgct
    11461 ttacctacct tggggcaggt gttcaagttc ccagtgcgcc tgacggttcc aacaattggg
    11521 aaccaagttg gacgtatgtc aaaagcgatg acacctttgg tttgattcat ggcgaatacg
    11581 atctcaccaa taattggaca ctctatggaa gtttcggagc acgaagaaac gacttcaaag
    11641 gcgtctatgg acaatcaacc atcactgata gcactggaga ctttaccggc acgtactata
    11701 cccaacccac atgggccgag agctacagtg ggcaaattgg cgttcgtggc catctgacca
    11761 ccggcccagt ggaacatgat attgatcttg ggtattccaa tctagattgg aacttcggtg
    11821 gtttattgac ctatttaccc gggacttatt cctccaacat ctatagcccc gtttcgcttt
    11881 cacaacccga cctatcaagc gtcaatctac acgtattcag gacgagccat atactgctgc
    11941 gaagctatgc gttcgccgac acgctttcca ttctcgacaa gagagtccag ttaacgcttg
    12001 gtgggcgcta ccaggaaata ggcgtcgaga acttcgatca aaacacggga attctcagtt
    12061 caagctacga ccgccgtgcc ttgacgccgg cagtcgggct agtagtgaaa ccgtggcaga
    12121 aagtctctct gtacgccaac tatattgagg gccttcaaca aggccccacc gcaccaactg
    12181 gttcgacgaa cgtaggggag gtgtttgccc cgatcaagtc caggcagatg gaagcaggtg
    12241 tcaaggtcga ctttggccat atagggacaa cgctgagcgt attccagatt aaacagccca
    12301 gcggcatcac caatcccact acgtttcact acgatatcaa cggtgaacag cgtaatcgcg
    12361 gtgtcgagtt caatacattc ggtgaggtat atcctggcgt ccgaattctc ggcggagtcg
    12421 cattcatcga tgcgcaaatg acaaaaactg cgggtggaac gcttgatggc aaacgtccag
    12481 tcggggtgcc cacctttcaa gccaacatcg gagcagagtg ggatacgccc tttgtcaaag
    12541 ggctcaccct gtccacacgc ctagtccaca cgaatggcgc gtacgtagat tcgaccaata
    12601 cccgcgccac ttccggttgg gagcgggttg atattggagc gcgatacacg ttccatcgca
    12661 gcggaggaaa aacgatcatc cttcgcgccg ggatagacaa tattttcaac aagaattatt
    12721 ggtcggctac gcagtttggc atgctggaac tcggtgagcc tcgcacttat cggttatcgg
    12781 caacattcga tttttagtca tgcctgcccc taatgcgtaa atggcgcgga cgcttccata
    12841 ggcgggttcg gcccgtggtc cgtacggacg acggccgccg cgttgaacag cgggatcagg
    12901 gccagttcgg aaaggggttg cgccaggtct tccacgccaa tgggccctgg gccatctctt
    12961 tatcggtcag caaacaggcc tcgaagcgcg cgcgcagcgc cgcttcatcc atgtccatgc
    13021 cgatcagcac cagttcctgg cgggcatcgc caacgccatc gacccagttc ctgcggatca
    13081 atgcaacgta ttccgggtcc tgcggccagc gctcgggcgg catggcggcc caccaatagc
    13141 cggccgcgcc gtggcgggcc acggcgcccg cctgggacca ggaacccgcc agttccggat
    13201 ggctggccag ccagaaatgg cctttggagc gcaccacgcc cggccattcg ctttcgacaa
    13261 aggcgaagaa ccgctccggg tggaagggac ggcgcgcgcg gtagacaaag ctgcggatgc
    13321 cgtattcctc ggtttcgggc gtgtgctggc cgcgcagttc ccgcagccag cccggcgcct
    13381 tggaggcctc gtcgaaatcg aacaacgccg tgttcagcac gcggtccaga ggaaccttgc
    13441 cgaactcgga cacttcgatc cgggcgcgag gattgagctt gcgcaggatg gccatcaacc
    13501 gctcgcgctc tgcctcgttg atcaggtcgg ccttgttgag gatgatgaca tcgcagaact
    13561 cgatctggtc gacgagcaga tccaccaccg tccgctcgtc ctcctcgccc agggattcgc
    13621 cgcggtcttg caggctgtcg cgcgaaccgt agtcgcgcag gaagttgtag gcatcgacca
    13681 ccgtcaccat cgtgtccagg cgggccactt cgcccaggct gcgcccatcg tcgccttcga
    13741 aggtgaaggt ttccgcgacg ggcagcggct cggaaatgcc ggtggattcg atgacgagct
    13801 gatcgaactt gccttccttg gcaaggcggc tcacttcgac cagcagatcc tcgcgcaagg
    13861 tgcagcagat gcagccattg ctcatttcca cgagtttctc gtccgtgcgg gacaactcgg
    13921 cgccgccttc gcgcaccagc gcggcgtcga tgttgacctc ggacatatcg ttgacgatga
    13981 ccgccacgcg ccggccctcg cgattgttga ggatgtggtt gagcagtgtc gttttgcccg
    14041 caccgagaaa acccgatagc acggtcacgg gcaagcgtgg gagggcagca gagacattcg
    14101 ttttcatcgt tggaggcctt gcgtatggga tttagcgggc aaagcggtac gtttcaccgg
    14161 gtcaggcagg gcagcttgcg ccaagccggc gcaggctgcg catcggccat ggatctcgat
    14221 agtcgaggac ttgggcgaaa aggcccgtcc gtcggcctgc cgctacaggc tcctgcccag
    14281 gatgcgatcg accagttcgt cgatgtgccc gcagttgtcg cacgacatcg agcacccgct
    14341 gctggtggga ggtcagttgc atcggcattg cctgtagcat cacgttattt gtgatgttat
    14401 aacataacat ataatcgacg gactggggtc tacccctggg tttggcgcag tcggacaaca
    14461 taggcctcgg catcgtcacg cgctcgcggg ccgagccggc ggtgtcgacc acctggccgc
    14521 tgtcggatca cgctagcaca cctcggtatg gatccagcag tatccaggcg attgtccagc
    14581 aagcatccgg ctaggcgcca gtgaatcgag gcggtcggtg atccaggaag tggctgacgc
    14641 cttccttgaa atcgtcgctg gaaatgctgt cgtacatttc acggttcgcc acgacagtcg
    14701 cctcggccag gccttggaac ggcgcttcgt agacttggcg cttcatcacg cacaacgacc
    14761 gcggggaaac caaggtcgcc aagtcgcgcg cgtaggccag cgcctgcggc atcagtgccg
    14821 cgggttcgca caggcggccc accagattca tgcgcaacgc ttcctccgcg cctatcttgc
    14881 gtgaagacag cagcaggtcc agcgcatggg cctggccaac gatgcgcggc agaatccagg
    14941 cgatcccgtg ttcggcgatc aggccgcgct tggcgaacgc ggtggcgaac acggccgccg
    15001 tgctggcgaa gcgcacgtcg ctatatagag ccatcaccag tcccaagccg gcggccgcgc
    15061 cattgatggc ggcaatcacc ggcttgcgca gcgcgggaaa atacccatag cgtgactggt
    15121 agtcagcgcg ccggttcatg tcatagggcc gcatccagcg ctgctgccgg atgtcgtcgg
    15181 gcgcaagcac ttccagttca tccatgtcca tgccggcgca gaaggcccgc cccgcgccag
    15241 tgagcacaat gacgcgcacc agaggatccg cgtcggcggc ctccatcgcg tcgcgcaact
    15301 cggcttccat gatggcggtc aatgcgttca tccggtccgg ccggttcagc gtgacggtgg
    15361 cgatccagtc ctcgcgggca cagacgagtg tttgatattc catgagaagt ctccgaggat
    15421 ggtcagtcgg gcatctgaat cgaatggggt tcgaggaatt cctcaatgcc aaagagtccg
    15481 ccttcccggc caaggccgga cttgcggaag ccgccgaaag gagccagcgg attgaaggcc
    15541 gcgccgttga cgtcgacctg tccggcgcgc atgcggcggg cgacttcgaa agcacgctgc
    15601 ggctgcgcac tccataccgc gccggccagc ccatagtccg tgtcattggc gatggccacg
    15661 gcctgctcct cgtcatcgta gggaatcacg cacaataccg ggccgaacac ttcctccctg
    15721 gccacaggtt cgctgccgtt ggccagcagc accagtggcg gcacgaagaa acctgtgggc
    15781 ggcagcgcca tcccggcatt gccggcgaag acttcgcaac cgtcgtccat ggcccggcct
    15841 gccatgccgc gcaccctggc ctgctgctgg cggctcacca gcggtccaag ccgcgtggcg
    15901 cgatccatcg gatctcccat gacgtacttg cctatcccct cggccaaccg ggtacggaac
    15961 acctccagca tcgcgcgtgg cacgagcaac cggctaatgg cgttgcagga ctgaccgctg
    16021 ttgagcatgc acgccgacag cgcatggcgc gccactgccg ccggatccgc atccggcaaa
    16081 gcgatgcaag cggacttccc gccgagttcc agggccagcc gcttgatctg tccgccagcg
    16141 atcgagccga cctgttctcc tacgcgcgtg gatccggtga acgacaccat atccacatcg
    16201 ggatgcgaga tcagcgcccg gcccgtgcca gcgccatcgc cccacacgag attgaagacc
    16261 cccgcgggca gtcccaccgc ctcggccgcc ctggcaagca cgaaagccga ggcaggcgcc
    16321 agctcggaag gcttgagcac gacggaacag ccagccacca gtgccggcgc gaccttgctg
    16381 gtgatctggt gcagcggata gttccatggc gtgatgcagg ccaccacgcc gataggcgcc
    16441 tggctgaccg tggaatgacc gatccgcttc tgccattcca cggttccagc cagctcggcg
    16501 tatctgcgcc aggccttcac cggcatgtcg acctggatta tcgatgacag gttcaggggc
    16561 atgccgactt cccgggtgat ctgcgcagcc agcgactgcg cttcggccgc caaccggtcg
    16621 cctatggcgt ggaccagtgc ctggcgctgt gccagcgggc tcgtcgacca actgtcgaat
    16681 gcctcgcggg ccgccgcaac ggcggcgtcg acatcgccgc cgcttgccgc acccacctcg
    16741 gccatcggtg cttccgtata ggaatccagc acggtggtcg cggcgccgcc ctggcaatcc
    16801 ttccacgccc catcgacata gttctgccaa tagcgctcgc ggtgcccctc gcctgccatc
    16861 gaatagacgg aactatgctc aaccatgctt ttctccaagt tagtccacgg atgcgccaga
    16921 ctgacgaacc gcctctcccc aacgttcgat ctcgcccatc agtctttcat tcgcctgctg
    16981 cacgctcccg cccatcatga ccaatcccag gttcgcgaac gtggcgcgga attgcgaatc
    17041 gcgcagaatc tcgttcatga cactgttcag cttatccacg ataggcttgg gtgtgccggc
    17101 cggcgccagg acggaaaacc aggtattggc ctcgtatccc tttatgccga gttcctgcat
    17161 ggtgggcaag tccggcacca aggacgagcg tgtcctccct gtttgggcga gtgcgaccag
    17221 ctttcccgca ctgacctgag gcagcgtggt gaggatggtg tcgatgacaa ggtccacctg
    17281 tccgcccaat agcgccgtga ccgcgggcgc cgacccctgg taggggacat gcgtcatttt
    17341 catgcccgtc gcgttctcga acatcaccgt agccaggtga ggcgtgccgc caatccccga
    17401 ggtaccgaag gacaggggtt tgtgcttggc gcgatcgacg agttgatcga gcgtcttgat
    17461 atggctgtcc ttgttgacca ccaccagcat gggcgaactg gcgatgtcgc aaacgctcgt
    17521 gaaagccctc ctggtatcgt agggcaggtg cttcatcagg gtcgcattga tggcgaacgg
    17581 gggagcgatc cacaacagcg tgtagccgtc cgccggactg ttcgcgacga attccgacgc
    17641 gatcacggtg ccggcgcctg gcttgttcac gataatcacg gattgcccca tgcgcttgcc
    17701 catctcgatg gccaacccgc gcgacagatt gtcggacgcc ccgcccggcg tgaacggaac
    17761 gactaggcgg atcggcttgt ctgggtagga atcgttggcc cggctcgccg atacgggcaa
    17821 cagcgccgtc gccagggccg cgacgaccgc cgccggaaaa atcttcaatg ccttcatgat
    17881 gtctcctcct gcataggtgt tgttctattg ttcatttgcc catgtatcta ggctttctct
    17941 tttccaggaa cgccgccacg ccttccgcct tgtcggcgct cgtttctatg gcgcgcaata
    18001 cctgcatggt gcgcgaggcc tgctcggatg ggctggcggc cagcatctcc tgattgacga
    18061 agcgtttgag cgcagccagc accatcggcg cgtagcctgc cagctcctcg gccatggcga
    18121 tggccgctgc cagatgggtc ccggccggga cgacctcgtt gaccaggccg acctcgtggg
    18181 cacgccgggc ggtcagcggt cggcccagca gcatcacttc catcgcgacc ttgtgcggaa
    18241 tgcgcgccgc caagcccgcg atcatgcccc cggtcagccc cagcctggcc tcggggtaga
    18301 ggaaccgcgt cgtctccgac gccacggcca gatcggccat catggcgatg accagcgcgc
    18361 cgccaacgca ccagccagaa agagccatga tgatgggctt gtcggtctgg atgcccagcg
    18421 tgggcacgca gcgccagaat tcgggcatgt cgctgacgtc cgcccctgcg ctgaatgccg
    18481 actcgccctg gccggtcagc accgcgacgc gttgcgacga ctggtcaaag tgcgcgaagg
    18541 catcctgcag ctcgagcgcc atggccgcgt tgatggcgtt tctcttttcc ggcctgtcca
    18601 gcgtgatcac ggtcacgccc gagctgcggt tatcgatctg gatatgcggc atttcgtctc
    18661 ctttccttat atcttcggct tgggtttgtc atgtctatgt cctgtcgcgc ggtcggcatt
    18721 gctgcgcgat ccgcaggcgt gcgtccaccg cccagcattc gcggcgcccg acgatgagcg
    18781 gatttatgtc gcattcgaac tcgcttcccg ccaggtcgtg cgccagccag ctcacccgca
    18841 cgatcgcatc caccgccgcg tcgatggcca gcggcgatcc gcgatagcct tgcaggaccg
    18901 gccagatggg cagcgtccgc aacagcgcca gcacgtggtc tggatgcgtc ggtgcgcgcg
    18961 cgaccgccat gctgcgcagc agctcgacca gaatgccgcc ggcgccgaag acgacgatcg
    19021 gaccgaattg cgggtcgcgg cgcgcaccga cgatgagttc cagttcgccg cgcaccatcg
    19081 tctggaccga cacgccgagc agccgggcgg acggcaccgc ccgcgccgca ttgccgatca
    19141 atccgtcgta gcgttcgcga accgatgacg tgtcggccac gcccaccgcc accgcgcctg
    19201 cctcggtctt gtggacgatg tcgggcgaaa cgactttcat caccaccgga taacccatgc
    19261 gctcggcgat ctctaccgcc tgatcggcac tggccgcgat ctcgccctgg ttgactggaa
    19321 ttccatatct ggcgaagcaa gccttgctgt catcctcgcc gtagtctccc ggagaaagcg
    19381 aacgctctgg tccggcgccc ttcggcctat tggccggctg ctcctggaca cctggccttg
    19441 tgctcaggtc gcgccaggtc tcccagacgt gcgccgcctc gccgatatga ttcgtgtacg
    19501 gcagtttgcg ttcgctcagc agctcgcgca actcgtcgct ggcatgcccg gcctccaagg
    19561 tgaacagcac gggcttgccc tgcgcagccc cggcttcggg ctgagccctg gcgaaggcct
    19621 gcagccatgc gggaggagcc atggtcacgg gaatcatgac gccgtcggtc cgcgcgtcgg
    19681 tttccagggc ctgcagcgtc gcgttggcag cacacacggg gtcgaccgtc ttgcggctgc
    19741 cgaaatccac tggattgttc gcttggccct cggggtaatg ctgcgccaac agcgcgcgcg
    19801 tctgctcgga gaattccgcc agcttcacgc cgcgttcaga cagggcatcc gaagccagcg
    19861 caccgccgcc tccggacggc gtcaggatcg ccagcctgtc gacgcgccgt tcgccatagc
    19921 gcgccagcat ggcggccagc gtgaccatcg cgccaacatc actcatcagc gtgacgccct
    19981 cttcccggca cactgccgcg aaaaccgcat agtcgccggc gatgctggcg gtatgggaaa
    20041 aggccgcgcg actccccgcc tccgtgcgtc ccgccttcac ggccagccac ggcttgcctg
    20101 acgccttgac ccgtcttgct gcctgcacga agcgtgccgc gtccttcacg ccctcgatat
    20161 acgtgcagat cacccgggta tcggggtcat cggcaaggaa ctcgacgaaa tcgcacagct
    20221 caagatcggc ctggttgccg acggaaacgc cgtgactgaa gccgatgccc tgcgaccatg
    20281 cccgatcgaa gaaggtcgtc atcagcgctc cgctctggct cacgaagcct atgggctgct
    20341 gcggcagcgc cgcgatctcc agaaccggcg aggagcacag cgcgagccgg ttcactgcgc
    20401 tgatgatccc gaggcaattg ggaccgatca ggcgcatccc gtgcgtccgg gcaatctcga
    20461 cgatttcagc ctcggcctga gcgccggctg cgctcgcatc ggaaaaaccg gaagtgatga
    20521 tgacgcagca gcgcaccccc atcctggcgg ccgtctcgat ctgcgccttg atggcgggct
    20581 ggggaatggt cagtaccgcc atgtccggcg cctgcggcaa gtcgccaaga ttgctccagg
    20641 tttgcaggcc gaacaattgc ttgcgccctg gattgaccgg gtatatctgc cccgcgaacc
    20701 ggtggcgcag cagcatgcgc agcaatcgtc cgccgaactt ggtctgatcc tccgacgcgc
    20761 cgatcaccgc gatcgacgac ggctgcagta aatgcgcgag cgggcgcgag ctcgccgatg
    20821 cgtggttgtc gctcaatttg tctcctcctg tcgaacgatg tccatcgcgc ccggaccatt
    20881 ggcggtctct taacgcgaca aggcttttat ttttgttcta tatagtagaa catgcatcta
    20941 tattatgaaa cagactactc agccatatcg aacctgtcaa gatggaaagt aaaaaatgga
    21001 gaatgcgtcc gccggctcga ccgacgtcca ataatgcgcg ccgcacgagg gacgcgttgc
    21061 gagaccgcca tggatgcggg catggcgatg gcggccttgc gccgcgtatt gacacgcgac
    21121 ggcgcctgcc tctattctga aaatcaaaat ccagaacttt gttccaaata ttggaataaa
    21181 tggcaattgg ccaggattca tagatctgcc ctggctcaag caggcgccgg acaaaccggt
    21241 ccccagaaaa agagagacat gactcacccc acgccgaacc tttccccgat ccccctgacc
    21301 cggcaactgg cccggcaggc tctcgccttc gatgcgcgcg acatccccga ggacgtcaag
    21361 gaaatcgccc gccactgcct gctggactgg ttcagcgtcg cgctggcggc gtgggacagc
    21421 ccggcgctgc gggccctgga ggcatgggcc atgctcgaac cctcgccggg atcggccagc
    21481 ctgttgggat ctgctcagcg cgtatccacc tcgcaggccg cgttgatcaa cggcaccgcc
    21541 tcccacattc tggatttcga cgacgcacac ctcccttccc gcgtccatcc ttcggcgccg
    21601 ctgtggccgg ctatcctggc gctggcgcaa acgcgcaatc tgtctgggga acgggtgctg
    21661 gcggcattcg ccgctggcgt acaggtgcag agccgcctgg gcgccgtgat gggaaatagc
    21721 cactaccgcg aagggtggca caacacgggg acactgggaa cgttcggcgc gacggccgcg
    21781 gtcggccgcc tggaaggcct ggaagaagaa gcgctatgcc aagcgttcgg cttcgccgcc
    21841 acgctgacag gagggctgcg cagcgtcttc ggctccagcg ccaagccctt gcacgcaggc
    21901 cgggccgccg ccaacggcct gatggcagcc ggcctggtcc agcaagggat gcaaaccgtg
    21961 gccgacattc tcgagcggcc gggggggttc gtgcagactt atgcgcgcga tttcgatacc
    22021 gcgcagatcg aatcccccgc cggcctgtgg gagattcgcc gcattgtctt caaataccac
    22081 gcctcatgct acggcaccca agcccccatc gaagcggccc tggccttgcg caagcatgcc
    22141 gaccgtgcct ccacatgccg cgtcgacgtc catgtcgaac cccagtacat gacggtgtgc
    22201 aatatcgcca agccgtccag tgtctccgaa gccaagttca gcatcgcgca catggtggcg
    22261 ctcgcgctgg cgggccggga cacaagcaac gagaagaatc tttcaggcag cgcgctgcac
    22321 gatcccgaca tcctcgcgtg gcgcgacctc atccatgtgc atggcgaccc ggcgatggcg
    22381 cgcgccaacg cgcgcgtggt cgtccacggc cccggcggcg aagccgcgca tacccacaac
    22441 gccagccagc cgcaggccga tctggcgcaa cagcggcgca ggctgctgga aaaatcctca
    22501 gcgctgctgc gcccgcgata cggcgagcgc gccagcacgc tggcgcagga gcgtttactg
    22561 gccattgacc gccagtccga actggcggac tggctgcacc agctgcttgc cgaactgaat
    22621 caagccagca gggcctaacg ctcgcgcgcg acccagccgc tcaccgcgtt ggcgccgcgg
    22681 tatacccaag cagccgggaa atctggccgg cgatctcggc caccggcggc ccccactcct
    22741 tcatttggcg cgccttgagc ctgtcggcgg gtccggacag gccgacggcc gcgatcacct
    22801 cgcccgaagc atcgcggatg gccgcagcga caccgcacac ttgctcgcgc cattcgccgc
    22861 gattgatcgc gtatccctgg ctacggatgc gctcgagctc ggcatccaga tccgcggcgc
    22921 gagtaatgct cttggaggag taggcgcgca aggccgcctt gacctgggcg atcctattcg
    22981 catccgccca ggccagttgc gccttgccgg tggccacgca ttgggccggc gcgcgacctc
    23041 caaccgtcgt ataggaacgc accggttgag gactgtctat cttggccacg tacagcacat
    23101 cgattccgtc caacaccgac aggtgcacgg tttccgaagt atccgccgcc agtctttcca
    23161 gaaacggctg tgccacctga cgcacgtcca gccgggacag aacgtgcgaa cccagctccc
    23221 acagcttcat ggtcagctca tagcgccccg tatcgccggt cttgcgcgca aaaccttggt
    23281 aaaccaggcc ctgcagcagg cggtgcacat tgctcttggt cagttgcagc gtgtcggcca
    23341 gttccgtgac gccgcaggcg cgctcgctac gcgccagggt ctcgagcaat gtcaaaccct
    23401 tcagaaatgt cttgtccata tccagtgcaa tccgttgccg ctccagcccc cgcggccggc
    23461 tccctgatcg aagcggattc accggcatcc cgcaaaggat gcacgacatg gccgccaccg
    23521 ccgccgggcc ggtgcatttg attgtgacgc gcaattatac ggaattccag aaaatggaac
    23581 gccgtgtcac ttcatgccta agaaagtcgc ttgacactcc aaaccacgca gccctaagat
    23641 ttcctcgcta ttttcaaaac aatgttctat atattggaac aacagaggat caccgaatcc
    23701 acttgatgcc ggagacaaca tgtcagctgc aaaactgaag gaattcgtgc aacaggccac
    23761 ccgagtcatg gactcggtgg cggacgaccg ggcgcgcatg ctcgcgacgc tgcctatcct
    23821 ggaaagactc gtggccagcg acgactggct gcccgaagtg ttcgcccagc cgcatcccga
    23881 gcactatcag caatacctgc tctactgcga tccccacgag cgtttcagca tcgtcagttt
    23941 tgtgtggggg cccggccagc agacgccagt gcacgaccac accgtgtggg gcctggtcgg
    24001 catgctgcgc ggctcggaga catcccagcg cttcgaaatg cgcaatggcg cgctgcgcga
    24061 gggcgggctc gccacgttgg agcccggaca gatagaacgc ctgctaccca aagagggcga
    24121 catccaccag gtctgcaacc gctacgacga ccgggtatcg atcagcatcc acgtctacgg
    24181 cgccaacatc ggccgggtgc gccggcacgt cttcgatccg aaaaccagcc agagcaagga
    24241 attcgtatca ggcttttcat cggccatgtt gccgaacatc tggaactgac agccccttca
    24301 ccagaaaaca aggagaaaaa catgggctca tatgtcatcg acagcgaact tttccgcgac
    24361 cagttcggca ccgaagcgat gcgcgacatc ttcagcgacc gcaccacggt gcaacgctgg
    24421 ctggatgtcg aggccgcgct ggccaaagtc caggcccgca tgtccatcat tccgcaagcg
    24481 gccgccgacg agatccagcg caaggccaag gtcggcctca tagacttgca ggagctgaag
    24541 gcggaaatgg accgcaccgc ccatcccatc gttccgctgc tgcgggcaat gaagaagatc
    24601 tgcgcggacg atgccggcga atatatccat tggggcgcca ccacgcagga catcatggac
    24661 accggcacca tcctgcaact acgcgatgcg ctgcgggaaa tcgacaagtc tctgcgcgcg
    24721 gtcctggaca aggccatcgt gctggcacgc cgccaccgtg attcgacaat ggtcgctcgc
    24781 agccatggcc agcaagccct gcccattacc tttggcttca aggtcgcggt gtgggtcgcc
    24841 gagataaggc gtaacctcgc ccgcttggcg caaatgcgcg agcgtgtgct ggtcggccag
    24901 ttctccggcg cggtgggcac gctggcggcg ctgggagaac agggcataga agtgcaggaa
    24961 gcgcttttcc aggagctggg cctgaggacg cccgaaatca cctggcacgt ttcacgcgac
    25021 ggcacagccg aattggcctg cgtcctgggc atctgcgtgg gcacgctggg caagatcgcc
    25081 cacgagatat acagcctgca gaaaaccgag accgccgaac tggaagagcc gttctctccc
    25141 ggaaaggtcg gcagcagcac catgccgcac aaacgcaacc cacccacctg cgaaaccgtg
    25201 gtggccattt cccgcatcgt ccgcagcacg gtgccgctgg cgctggaaac catgatagcc
    25261 gagcacgaac gcgacaagat cgcgcttcag accgaacggg aattcgtctc gcgcatgtgc
    25321 agcatgacgc acgcggccgc gcagaagatg acttttgtcc tggacaagct cagcgtcaga
    25381 accgacaaca tggagaagaa cctgtacatc cagaatggcc tgctgctttc ggaagccgtc
    25441 atgatgcact tgagcgacgc gctgggcagg caggaagcgc acgatatcgt ctatcgcgtc
    25501 tgcatggacg tatttgaacg cggaggaaac ctgaaggacg cgatcctgag tgatcctcag
    25561 ctcaagggca ggatcagcgc gcaggatctg gaccgcgtgc tcgatcctca cgcctacaca
    25621 ggcctggccg gcgtcttcgt cgaccgcgtg ctgagccgcc ccgcgcccgc ggcccccgga
    25681 gactgacacc ggacacgaaa tccggcgcgc cagcccgcgc gaacgcgcgc cgccccaccg
    25741 gcggcgcgcg ttcgtcattt ggagaactga tcgaacgcct ccatggcccg caccgagtag
    25801 atataggcgg cgcccgcgtt gagcgcgatc gccgtgccca acgcctcgcc cagttcctcc
    25861 tgcgtgacgc cagccttgcg ggcggcctcc acatgcgagg cgatgcatcc atcgcagcgg
    25921 gtcgtcaccg ccacggccag ggaaatcagt tcacgcgttt tggcgtccag cgccttgccg
    25981 ccggcctggg cctcgttcaa gccgcggtag gcggcaacga caggcggatt ggcgctggcg
    26041 aggagtcggg cggccgaccg gaccttggat agaaattcga cccagttgtt caacatgatt
    26101 cttgctcctt ttgaaaaaac aggcagcgac atcgccgcgc tgaaaacctg ccaagaaatt
    26161 gttctatata ttggaacaaa gttttaaaat ataaaaatat cgtacccggt cgaacgggag
    26221 ctggcaagaa ttgcggcacg cgaaggtttg acgagcactc ttgtcgccgc tatcctagtt
    26281 ctattattta tatatatgtt ccaaataata gaacaaagcg gaagccgagg atcacgatcg
    26341 catcggctcg ccgttcatgc ggagcttttt catgaccatg caaacgcaag acgactcgga
    26401 aaacagcgca cacccgcctc tgcggggcgt gcgcgtgatc gagttctgcc agatcgccgc
    26461 cggcccgttc tgcggcatgt tgcttgccga cttcggcgcc gatgtcacca aagtggaacc
    26521 tccagacggc gacgggctgc gccagtggcc ccccatcagc gacgggttca gcgagaactt
    26581 cgcctcgctg aaccgtggca agcgctctgt ctgcctcgac ctgaagaatc ccgcgcagcg
    26641 cgacctcgcg cgcgcgctcg tcctggatgc cgacgtcctg gtggaaaaca gcaggcccgg
    26701 cgtgatggcc cggctgggac tgggatggga ctggttcgga ccgcgcaagc ccggattggt
    26761 atattgctcg atctccgcct acgggcagac tggcccgcgc cgagccgaag gcggtttcga
    26821 tctcacgatt caagccgccg cgggcgtcat gagcgtcacc ggagagccag ggggcgaacc
    26881 ggtgaaatgc ggagtgccgg tttccgattt caccgccggc ctgtacgccg cattcaccat
    26941 ttccgccatg ctggcgcgcg tgcgcgcggg cggccccggc ggccatgtgg acgtgccgat
    27001 gttcgccacc accctcgcga tcgccgcgct gcagaccagc gaatatttcg gcacggggcg
    27061 caacccccgc aaactgggtt cggcgcatcc tcgcaacgcc ccttaccagg cataccaggc
    27121 aagcgacggc tggttcgcca tcgccgccgg caacaaccgc ctgtgggagg ccgtctgcgc
    27181 cgtggtcggg ggcgaagcct ggcgcaacga cccgcgcttc gtctccaacg cccagcgcgc
    27241 ggcgcaccag gccgagttga aggccctgct ggaccccttg ttcgcacgcc gcaaggtctc
    27301 cgactggctg gacgacttcc gcgccgccgg cgtgccctgc gcgcccatca atgattacgc
    27361 cagcgcactg gacgacccgc aggcacggca cttgcaactc acccagccca tggcgctgcc
    27421 cggcgggcac gccacgcgca cggtgggctg ccctgtctgg ctggacggtc ggccggtcga
    27481 agtgcaaacc ataccgcccg gcttgggtca gcacacgaac gacgcccggt cgcgctatgc
    27541 cttcgacata catgccgctg gcacacggga ggaccgatga ttcctccgga caacgccggc
    27601 gcgcccgcat gcctggagat acgcgcagcg gttgccacgc tctggctgaa ccgcgtgcaa
    27661 caggccaacg cgctcagcgc cgacctggtg gaagcgctca tcgcgcatac cacgacggcc
    27721 ttcgctgata cggcattgca caccctggtg cttcgcagcg caggccggca tttctgcagc
    27781 ggtttcgaca tgtccgatct agaacagcag agcgacggcg acctgctctg gcgcttcgtg
    27841 cgcatcgaga ccctgttgga cacgctgtgg aacgcgcccg tccgcacgat cgccgtagcc
    27901 gattcccggg cctggggcgc tggcgcagac ttgttcgccg cctgcgatct gcgcatcgcg
    27961 ctgccgcaag cgaatttcgc ctttcccggt tcacgcttcg gcctggtgct gggcacgcgc
    28021 cggctagctg cgcgcgtagg gcgcgaaccc gcggtcgaga tagctagcgc ggcccggata
    28081 ctggacgccc gggaagcgca ccgctgcgga ctggccagcc acctctgcga cgcagaggct
    28141 ctggatgatc tgctcgcgca actggccgga accggcactg cccagcgcga cgtcggtgaa
    28201 gcgctgcgct gcgccgcccg ggccggtgaa gccgcgctac gcgacagcga ccttgccgcc
    28261 ctggtccgtt ccgccacgcg ttccggcctg aaacagcgcc tggccgatta ccgcgcggca
    28321 gtgcgccgca gcgccggacg ttaaccacat gccgggccga cgcctggcaa tgcacgcaag
    28381 tcaccaaccc aggctttgac ggcgctacgg acgtctacgc agccggcaag tcgttgcgag
    28441 gagcgcattg catgcgcccc ttttcatttt tgcgacaccg tcagcaccat cagcacgtcc
    28501 gcataccacg cgcaattcaa gcccagcgac tcatcttcca cctggtcgcg cgtgcgcata
    28561 tccatgcggg tggagtgcac gccgagcaat tgccatcgaa gcggggaatc gtccccggcg
    28621 tgctcgcccc tgcggcgcag gacgggggag ccgctgctgc cgcgatgcgt cctggcgtcg
    28681 gtcagaaaat agccttgctg ctggaatcgc acgccgtatg ccgacgcgat cgaagcgctg
    28741 cgcgcgacgg cgagatggtg aatcgtgtcg tgaaaaccca agggaaagcc gatgaccgtc
    28801 aaggcatctc cgaccgccac ctcctcgccg cgggggtcga gattcgacgg gtcgaatgcc
    28861 tgcagcatca cgtcttcggg caaacagtcg gcatcgatct cgatgaccgc gatgtccacg
    28921 gcgccggcgc tatcggcggc ctggcgccac aagcctcgcc cgccgcgata gagcgggatg
    28981 gaaaaagtcg cgtaccgcgt cagatcgcgc gcgtcgatat gcacttcgat ttcgatccgg
    29041 tccggacaat ggccgcttgc ttcatcgaca aagacatgcc ggttcgtcac caggaacaag
    29101 cgctgatccc ggcggaaaaa gaagccgctc gcgccggaca ggggttgccg gcccaggaag
    29161 gtgctgacac gcgtcgcggt cagcagcacc gcttcggcag cgttgcccgg gtccggcgga
    29221 ctgtccgccg atacgatccc ggccaatacc gcttgggtcc gggcaatgtc gcgctcgaag
    29281 gcggccaata catgatcgga aatgtcggcg ttcagcatga ccgtgcgcgc cgcctcggca
    29341 tgcgcctcga acgaacgcgt cagggcatcg tggacactcg atggcaaggc ttccagcaaa
    29401 gccggaagca aggcatccat cgcgaccagg ctccccttga gttcacagat gcgctcggtg
    29461 gcttcatcca gatttttcat ctcgagaacc ctccaaagaa taaaaaagcg cggacaagcc
    29521 gcgctttggg agaaaacgtg ggaacagctg cagggccagg ccctgcatcc gctcaactca
    29581 cggcacggat gttggaggct tggggcccct tttcaccctg cgtgacttcg aattgcacgc
    29641 gctggttttc ggtcagcgtc ttgaaggcgc ccgactggat ctcacggaag tgggcaaaca
    29701 gatccttgcc gccatcatcg ggagcgataa agccaaagcc cttgccttcg ttgaaccatt
    29761 tcacggttcc gatttgtgtc gtcattttga ttccctttct aatttgccag gacgcaaagc
    29821 cgcgcctgac gaagaaacgc caagataatc atcaagggaa atcgacacag ccgccgacag
    29881 aatgcccggc gggaagcaga aaaggcctat gaattgatag tcttgcacat atcaacagcc
    29941 ccatgctaca tggctttcgc ctggcgtgcg gattcgccgg atatatattt tcaggggctg
    30001 gatcggcaca tagatgggtc aggcgcgcag gatgcgcgcc tgacctcgtg ccgcgacagg
    30061 acctacagcg tcgcgatgtc gatgacgaag cggtatttca cgtcgttctt gagcatgcgc
    30121 tcgtaggccg tgttgacgta agccatgtcg atcatctcga tgtcgctcac gatgccgctc
    30181 tcggcgcaga agtcgagcat ttcctgggtt tcggccatgc cgccgatggc cgagcccgcc
    30241 agcgacttgc ggccgtggat caccgaaatg ccggtcggcg gcggggtgag cgcggtaagc
    30301 gcgcccacca gggccatcgt cttgtcgagt ttgagcagcg acagataggg attgacgtcg
    30361 tgcccgaccg ggatggtgtt gagcaggaag tcgaagctgt tggcatgttt ggccatggca
    30421 tcggcatcgc gcgacagcag gacttcgtcg gcgcccaggc gcgtggcgtc gcggcccttg
    30481 tcgggcgagg tggtgatcat caccacgtgc gcgcccatgg ccttggcgaa cttgatgccc
    30541 atgtggccca ggccgcccag gccgatgacg ccgaccttct ggccgaggcc gaccttccag
    30601 tggcgcagcg gcgagtaggt ggtgatgccg gcgcacagca gcggcgccac ggccttgagg
    30661 tcgagcttgg gcgagatctt ggcgacgaag tgctcgtcga ccacgacctg ctcggaatag
    30721 ccgccgaagg tcagcttgtc ggtgccgcgc tcgacgtcgt tgtaggtcag cgtggcgccc
    30781 tgctcgcaat actgctcgag atccaggcgg caggccgagc agatgcggca cgactcgacc
    30841 atgcagccca cgccggccag gtcgcccacc ttgaacttgc ccacttcgtc gcccaccgcg
    30901 accacctcgc ccacgatctc gtggccgggc accatgggat agcggggatt gctccagtcg
    30961 ttgcgggcct ggtgcaggtc ggagtgacag atcccggcat acaagatctt gatctgcaca
    31021 tcgttggggc ccggttcgcg gtgctcgaat tgaaagggaa ccagcggcga ttgggcatcg
    31081 tgcgtggcgt atcctcggac ttgaatcatg atgtgctcct tgtaacgacc cgggtttacc
    31141 ccgaaagata gaacaataaa gggctgcgct ccccctagac aagcttgaaa attggcatga
    31201 tttgatgaag aatcctgcat aatcatgcag gcagacaatc tcgcccacct ggccgccttc
    31261 gccgcggtcg cgcgccacca cagctttcgc aaggccagcg ccgaactggc cctgtctccc
    31321 tcggcggtca gctatgccat ccgcagcctg gaggaacggc tgggcgtcaa tttgttcaac
    31381 cggaccacgc gcagcgtgtc gctgaccgcc gccggccagc gcctgctcga gcgcatccag
    31441 cccgccctgc gcgacgtggg ccatgcgctc gacgagatga acgcctttcg cagcacgccc
    31501 accggcacgc tgcgcatcaa tacctcgcgc gcctcggcct acctgctggt cgcgcccctg
    31561 gtgccgcgct tcctcgccgc ctacccggac atccggctgg agatcgtcga cgacgacagc
    31621 ttcgtcgata tcgtcgccga gggatacgac gtgggcatcc gcttccagga agccgtcccg
    31681 gaagacatgg tcgccgtgcc catcgccaag gcgcaacggt ttgccctggt cggttcgccc
    31741 gcctacttcg cgcggcatgc cattccccgg cacccgaccg acctgctcgg ccacgactgc
    31801 gtgcgccacc gatttcccag cggccggatc tacaaatggg agttcgagcg ggacggcgcc
    31861 aagctggaga tcgatccgcc cgggcgcgtc acgctgggcg acctgcacct gatgacgcgc
    31921 atggcgctcg atggcgtggg cctgaccctc cagttggaag aacacgtccg cgacctggtc
    31981 gaatcgggcc gcctgctgcg gtcgatggag gactggtgcc cgcccttccc gggattcatg
    32041 ctctattacc cgaggcagca gcgcatgtcg tcggccctgc gcgccttcat cgacatggtc
    32101 cgcgaaacct gaacccgcct cagccttccc ccgccgggtc cgggctggcc ggcggcacgg
    32161 gcggggccag cgcctcgctg tcgtccttga ggcccacgcc ggtgagcttg cgccgcaagg
    32221 cctgccgcac caggtagtcc agcttgccgg cggccgcgtc gatcggcagg ccttcgggcc
    32281 gcacattgga aatgcagttg cgctcgctgt ccaggcggcc ggtgcgcggc gcgtacgtga
    32341 agtagatgcc caggctgtcg ggcgcagtca ggccgggacg ctcgccgatg aacacgatga
    32401 ccatccgcgc ccgcaaggcc tcgccgatat cgtcgcccag cgccacgcgc ccctgctcgg
    32461 ccaacacgat gggggtccgc gcccaatcgg tgtcgtagca gcggcgcaag gcatcgagca
    32521 gcgggacggc gtggcgttgc acggccacgg ccgacaggcc gtcggccatc acgatggcca
    32581 gctccggcgc cggggccgcg gcgcgcaggg cctggccgtc ggccggcgcc aggcggcggc
    32641 ccaggtcggg acgcagcagg taggtgtgcc ggtcgccggc ctggctgcgc acccgcgcca
    32701 cgcgcaggcc gcgctcgcgc agttgcgctt caagcgcgtc ggcgtcgagc ggatgatgca
    32761 cggcatcgcg cgcccgcgca tgggccaggc cgaatttgag gatttcggcg ctgggcaggc
    32821 tggcccccac gcggcccagg gcgatgcgcg ccgacgtatg cctgcgcaat aggtcccagg
    32881 gagagtcctg gaccagcgag tcgggggatg ggctcacggc ggcctcagga cgcggcaagc
    32941 cgcggcaact gcctgatgag cgcgttgccc gcgccgacat cgagcaggcg tccgccggca
    33001 tcgacgatgg ccatgcgctc cagccaggcc tggaactcgg gcgcgcgccg cttgcccagc
    33061 acctcgcgca gatagagcgc gtcgtggaac gaagtgctct ggtagttgag catgatgtcg
    33121 tcggcccccg gcacgcccat gatgaagttg atgccggccg cgcccagcag cgtgagcagc
    33181 gtgtccatgt cgtcctggtc ggcctcggca tgattggtgt agcagatgtc gcaacccatg
    33241 ggcacgccca gcagcttgcc gcagaagtgg tcctcgaggc cggcgcggat gatctgcttg
    33301 ccgtcgtaca ggtattcggg gccgatgaaa cccaccacgg tattggtgag cagcggcttg
    33361 aaatgccgcg ccaccgcgta ggcgcgcgcc tcgcaggtct gctggtccac gccgtggtgc
    33421 gcgccggccg acagcgcgct gccctgcccc gtctcgaaat acatgacgtt gtcgcccacc
    33481 gtgccgcgct tgagcgacag ggccgcctcg cgcgcctccc gcagcagggc cagcgaaatg
    33541 ccgaagccct ggttggcctg ctcggtgccg gcgatggact ggaacaccag gtcgacgggc
    33601 gcgccctgct cgatggcggc gatctgggtg gtaacgtgcg tgagcacgca ggtctgggtg
    33661 gggatgtcga aatgcgcgcg cacctcgtcg agcatgcgcc atagcgccat cagcgccgcg
    33721 ggcgaatcgc cggccggatt gacgcccacc acggcgtcgc ccgcgccgta cagcagcccg
    33781 tcgagcatgc tggccgcgat gccggcgatg tcgtcggtgg gatggttggg ctgcagccgc
    33841 accgccaggt gcccgggcag gccgatggtg tcgcgaaaac gcgtgacgac ctggcatttt
    33901 ttcgcggcca ggatcaggtc ctggttgcgc atgagcttgc tcgccgccgc cgccatctcg
    33961 ggcgtgatgc cgggcgcggc gcaagccagc gccgcgctgt ccgtgctttc cagcagcagc
    34021 caattgcgga actcgcccac cgtgagatgc gcgatgggtg cgaaggccgc ctggtcgtgc
    34081 gtgtcgatga tgagccgggt gatctcgtcc tgctcgtaag ggatgagcgc gtcgttgagg
    34141 aaatcgcgca agggcacgtc ggccagggcg atgcgcgcgg ccatgcgctc ttcgtaggat
    34201 gcggccgcca gcccggcgag ctggtcgccg gagcgggccg gcgaggcctt ggcaagcagg
    34261 gtcttgaggc cgtcgaactg gtagcccagc gcttggaagc gggtgtggta cgccatgcga
    34321 atcgtgcctc ggcctgcggg ggtgggttag tctgtgtcat cttgccacct tcccccacgc
    34381 ccgactagat gccgcgttcc gccagcacca agaacccttc cgaagccgcc gccgagaagc
    34441 tgctggcgtt tcgcgtgttg tccctggggc agttgctcaa ccgcggcatc gccgtcctgc
    34501 tcgaccgcca cctgggactg tccgtgcggc aatggcgcgt gctgttctac ctggccaatt
    34561 cagggccgga ctctccgcag aacatcgcgg ccttctgccg ctacgacaaa agccaggtca
    34621 gccgcgcgat cgccgagctg caaggcaaga agctggcacg caccagcgcc agcgccaccg
    34681 acggccgccg cctggaggtc tcgctgacgg cggcaggccg tgccgcctat gcgcgcggcc
    34741 tgcccgtgag ccaggcccgg
//
LOCUS       DASWPO010000252         7689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3005773, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000252 DASWPO010000000
VERSION     DASWPO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005773"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGC69_13255"
     CDS             complement(<1..473)
                     /locus_tag="VGC69_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0296291.1"
                     /translation="MKTRFAFKRACTTLLLGAAALAAMPSGAQERAPGTLKVAAFGGT
                     FTAAQKKYIGDLFTRRTGMKVQYIDGNANEFIGQLIASKGRTPPYDVLYLEQDVRDSA
                     ISAGVFAKADPAIVTNLKYLYDEAKQPDGYGPGMAFYSVGIAYNTKKFAEAGIPAP"
     gene            complement(528..1541)
                     /locus_tag="VGC69_13260"
     CDS             complement(528..1541)
                     /locus_tag="VGC69_13260"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0296292.1"
                     /translation="MNDRYDMISERSAWRSGLIGGKEGLTMVLTPTHLAALDELMNRT
                     RNQPCESITREQFDHPDLTPFLKQVSDELSKGRGAVILKTLPIDRYSEDELTRMFWGM
                     GVHLGVPAEQNTAGDRIDHVRDMPSDNPLGRRQYSTQELVLHTDSPVGEVLALLCMQT
                     SKSGGRSQLASALAVHNEMAARHPELLDALYVGFPYHRKGKQRAGDPLVTPFPVPVFS
                     KIDGLVSVRYIRDYMEKAALNMGVDMPAVTRAALDKFDEISNREDMSVTFMLERGEIM
                     LVNNLTSLHARTEYEDHDEPEKKRHLVRLWIRVPNGRPYKPEMDLMYNDDPTKPSDAA
                     VMS"
     gene            complement(1905..1981)
                     /locus_tag="VGC69_13265"
     tRNA            complement(1905..1981)
                     /locus_tag="VGC69_13265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1945..1947),aa:Arg,seq:tct)
     gene            complement(2079..2921)
                     /locus_tag="VGC69_13270"
     CDS             complement(2079..2921)
                     /locus_tag="VGC69_13270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEY0296293.1"
                     /translation="MLKSLFRKQPNPAPAAEARADEPLALRLPSVEMLDGQPGELATL
                     LEIAHLLRSVRDEQSLTLISLPDDHVSSGVTTVLEVGLQANILVVAGLPDSELTQKML
                     EATGPLMVSTSFDQVYITFEVDGLEATDHFGRPALKARLPKSVTYMQRRDAFRIGVPS
                     FKGVSCTILQNDRPIHPTAGKLDVRDLSVIGLALSDAARVLDTTPGKVYDARLDIPHL
                     GAFDIILSVVHATDEESAGKGVAKARRVGCAFVDMPQGVRIRIQSFIGGLQREEIMRQ
                     RGLL"
     gene            complement(3036..3698)
                     /locus_tag="VGC69_13275"
     CDS             complement(3036..3698)
                     /locus_tag="VGC69_13275"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEY0296294.1"
                     /translation="MDADFWLERWRRGQTGFHLTRVTPLLAKYWPVLGVPRGSQVLVP
                     LCGKSLDMAWLASQGYRVLGVELSPLAVEQFFAENQLSPQKRAMPAGTLYSAGGIEIV
                     CGDIFALGQADFAGCAGAYDRAALVALPPPMHERYVRHVYGQLPAACRTLLLTLEYDQ
                     REMAGPPFAVLEDEVRDLYASCAGVAVLDRRGILDKEPSFAARGLTRLETVVYELRRD
                     QV"
     gene            3836..5227
                     /locus_tag="VGC69_13280"
     CDS             3836..5227
                     /locus_tag="VGC69_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296295.1"
                     /translation="MPLTSLGPLIVPLIVACALLMENVDSTILVTAIPVLARDLGHDP
                     VTLKLAVTSYVVGLGVFIPICGWVADKFGPRTVFRTAIATFVIGSLLCAAATSLTTLV
                     LARFIQGVGGAMMVPVGRILMFRAVPKSDFIRAVTYLTIPAQLGPVLGPLLGGFITTY
                     LNWRLMFFINVPIGILGIYLTNRYIENNREEDPGKLDWTGFFLSGAGASLFMLGMSMI
                     GSGLIQEGTAVALTLAGLLILGIYWIYSQRVENPLLELRFLHIPTFQCSVLGGSLFRI
                     GLGAMPFLLPLALQDGLGMTAFEGGFITCCSAIGSFGIKTIAIKVLKRWGFRNVLMVN
                     AVLVGISVAVCGVLHPGMSRLTIWLIVTLGGLFPALQFTSLNSLAYADIESRDIGRAT
                     SVASVIQQMSLGLGVTVAGMLLHFSQALHGRPEIDWTDFWPAFLVVGMFSVASVAFTR
                     RMPPDGGSGVTGR"
     gene            5336..6088
                     /locus_tag="VGC69_13285"
     CDS             5336..6088
                     /locus_tag="VGC69_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296296.1"
                     /translation="MTTTSAPLHGQKALVTGGSTGIGAALCERLIDLGAEVVSLSLAD
                     RADFSHPRFTQRAVDLSHPAAVKAAAARIAQEGDYDILVNNAGRVIHKPLEEVTDQDF
                     DDLMNLHVRAAILLTQAVAPGMKQRRYGRIVNLATRAIVGLPGRTIYSASKAAIVSMD
                     RTWALELAPYGITVNTISPGPIGTPMLNEGLPEGDPKRDALAASVPLKRLGTAEDVAR
                     AIAFFIDPASSWITGQNLFVCGGSSIAASLAL"
     gene            6133..6846
                     /locus_tag="VGC69_13290"
     CDS             6133..6846
                     /locus_tag="VGC69_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296297.1"
                     /translation="MAITWFCVWQGKGGTAGARVDEYDLNALKALVRGCPGLLAGHLL
                     TPAQAHDPHYAGEQEQAQPALILQLDFADALALETLLRPGAHLSVLAEPKFLPSLKDV
                     PAAQQAMLARRYAVADRQIASPDGSSLSYWVEYAGPAQDENAWHAFYVTHHPYLLAKF
                     PGIRAIDIYTPAVAICELPLPVRPCLQRNKVVFDTPEAMTAAMQTPVRDALRADFHEF
                     PAFTGHALHFPFKTWTYRP"
     gene            complement(6870..>7689)
                     /locus_tag="VGC69_13295"
     CDS             complement(6870..>7689)
                     /locus_tag="VGC69_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0296298.1"
                     /translation="VADRAGVRRIAFEALDSLRAADRTLYCFDVRTPEEYEAGHLPGF
                     APAPGGQLVQETDHNAPVRGARIVLADDDGVRASMSASWLAQMGWEVYAVDPAPDKAA
                     HVPGAWKRAVPEPPAAETVSVATLAEWLESGDSTQVLDFAPGVRYIKAHIPGAWFALR
                     AQLKQALRAAPRPRRYVFTCGSSLLAYFAAREARALTDVPVYVLDGGNAAWITAGHAV
                     ESGETRLASPRIDRYRRPYEGTDAPRQAMQAYLDWEFGLVEQLGRDGTHKFNVI"
BASE COUNT         1276 a         2663 c         2401 g         1349 t
ORIGIN      
        1 ggcgccggga tgcccgcctc ggcgaatttc ttggtgttgt aggcgatgcc caccgagtag
       61 aacgccatgc ccggaccgta gccgtcgggc tgcttcgcct cgtcgtacaa gtacttcagg
      121 ttggtgacga tggccgggtc cgccttggcg aacacgcccg cggaaatcgc cgaatcgcga
      181 acatcctgct ccaggtacag cacatcgtag ggcggcgtgc gccctttgga agcaatcagc
      241 tggccgatga actcattggc attgccgtcg atatactgca ctttcatgcc ggtgcggcgc
      301 gtgaacaggt cgccgatata cttcttttgg gccgcggtaa acgtgccgcc gaaagccgcc
      361 actttgagcg taccgggagc gcgttcttgc gcgcccgacg gcatggccgc cagcgcggcg
      421 gcacccagaa gcaaagtggt gcatgcccgc ttgaaagcaa aacgtgtctt cattttccat
      481 tcccctgcca tcgacggttg atggtccgct tttgttgtgt ttggccttca ggacatgaca
      541 gccgcatcgc tcggcttggt cgggtcatcg ttgtacatga ggtccatttc aggcttgtac
      601 ggccggccgt tgggcacgcg gatccacagc cgcaccaggt ggcgcttttt ttccggctcg
      661 tcgtggtcct cgtattcggt acgggcatgc agcgacgtca ggttgttgac gagcatgatc
      721 tcgccgcgct ccagcatgaa ggtcacgctc atatcttcgc ggttggagat ctcgtcgaac
      781 ttgtccagcg ccgcgcgcgt cacggcgggc atgtcgacgc ccatgttcag ggcagccttc
      841 tccatgtagt cgcggatata gcgcacgctg accaggccgt cgatcttgct gaaaacgggt
      901 accgggaacg gcgttaccag cggatctccc gcgcgctgct tgcccttgcg atgataggga
      961 aaacccacat acaaagcgtc cagcagctcg ggatgcctgg ccgccatctc gttgtgcacg
     1021 gccagggcgc tggccagctg gctgcgcccg cccgacttgc tcgtctgcat gcacagcagc
     1081 gccagaacct cgcccacggg gctatccgta tgcagcacca gttcctgcgt gctgtactgg
     1141 cgccggccca gcgggttgtc cgaaggcatg tcccgcacat gatcgatgcg gtcgccagcc
     1201 gtgttttgct ccgccgggac gcccaggtgc acgcccatgc cccagaacat gcgcgtcagc
     1261 tcatcctcgc tatagcgatc gatcggcagg gttttcagga tgaccgcgcc gcggcccttg
     1321 ctaagctcgt cggacacctg cttcaggaac ggcgtcagat cgggatggtc gaattgctct
     1381 cgggtgatgg actcgcaggg ctggttgcgc gtgcggttca tcaattcgtc cagcgccgcc
     1441 aggtgcgtcg gcgtcaagac catcgtcaaa ccctccttgc cgccgatcag cccgctccgc
     1501 caggcagagc gttccgaaat catgtcgtag cggtcattca tttcccaatc tcccaagacg
     1561 agtaatttat taactgtcta gtcaaccaat ccaatcatcc aacaactcat atgcgattcc
     1621 aagccctggt ttacccttaa atatccatga ataaacacgc gccgccgcgt gtatttcgcg
     1681 gaaatacgca tctcgtcgat catcataaat tgactattta gttaattttt taacttgctc
     1741 cgggaaaccc tgccgggcct ttccaaggca caaggccgcg cgatacgcga gccagcgcgc
     1801 agaaacgccg ctgaaaaacg aaaagcccat cgggcgcaga gacgcaaccg atgggctcga
     1861 ataattttgc ttcaggaaat cctgaatccg ccaggcaggc gcattggccc gccctacacg
     1921 attcgaacgt gtgaccgctc gcttagaagg cgagtgctct atccaactga gctaagggcg
     1981 ggaaaagaca atgcggcttg cggcgggatt ttaccgtgcc gcagccgccg gcaggccgcc
     2041 aagggtgaac gggccacccg gtgccctgcc ccgccccgct acagcagtcc gcgctggcgc
     2101 atgatttctt cgcgctgcag tccgccgatg aagctctgga tgcggatgcg aacgccctgc
     2161 ggcatgtcga cgaaggcgca ccccacgcgg cgcgccttgg ccacgccttt gcctgccgac
     2221 tcttcgtcgg tggcgtggac cacgctcagg atgatgtcga acgcccccag gtgggggatg
     2281 tcgagccggg cgtcatagac cttgccgggc gtggtgtcga gcacgcgggc ggcgtccgac
     2341 aaggccaggc ctatcacgct caggtcgcgc acgtcgagct tgccggccgt cgggtggatc
     2401 ggcctgtcgt tctggaggat cgtgcagctc acgcccttga acgacgggac gccgatgcga
     2461 aaggcgtcgc ggcgctgcat gtaggtgacc gacttgggca ggcgggcctt gagcgccggg
     2521 cggccgaaat gatccgtcgc ctccaggccg tcgacctcga acgtgatgta gacctggtcg
     2581 aaggaggtcg agaccatgag cggccccgtg gcttccagca tcttctgggt cagctcggaa
     2641 tcgggcaggc cggccacgac caggatattg gcctgcaggc cgacttccag cacggtcgtg
     2701 acgccgctgc tgacatggtc gtcgggcagg gagatcagcg tcagggattg ctcgtcgcgc
     2761 acggaccgca gcaggtgcgc gatctccagt agcgtggcga gttcgccggg ctggccgtcg
     2821 agcatctcga ccgaaggcag cctgagggca aggggctcat cggcgcgggc ctctgcggca
     2881 ggcgccggat tgggctgctt gcggaataaa gacttcagca cggacttccc caaggcgttt
     2941 cgagccattc ctggccaggg acgggctact catgaataaa tgtaacaact tgggaaagaa
     3001 tcttaccaga gcccaagggc tttggctgtg acaaatcaga cttgatcccg ccgcagttca
     3061 tagaccacgg tctcgagccg ggtcaggccc cgggcggcaa acgaaggctc cttgtcgagg
     3121 atgccgcgcc ggtcgagcac cgcgacgccg gcgcaggacg catacaggtc ccgtacttcg
     3181 tcttccagca ccgcaaaggg cggcccggcc atctcgcgct ggtcgtattc gagcgtcagc
     3241 agcagggtcc ggcaggccgc cggcaattgg ccgtagacat gccgcacata gcgctcgtgc
     3301 atgggcggcg gcaaggcgac cagcgcggcg cggtcgtagg cgccggcgca tccggcaaag
     3361 tcggcctgcc ccagcgcgaa gatgtcgccg cagacgatct cgatgccgcc cgcgctgtac
     3421 aaagtgcccg ccggcatcgc gcgtttctgc ggagaaagct ggttttcggc gaagaactgc
     3481 tcgacggcca gcggcgagag ctcgacgccc agcacgcgat atccctggct ggccagccag
     3541 gccatgtcca gcgacttgcc gcacaagggc accagcacct ggctgccccg gggcacgccc
     3601 aggaccggcc agtacttggc cagcaggggc gtcacccgcg tcaggtgaaa ccccgtctgg
     3661 ccccggcgcc agcgctccag ccaaaaatcc gcgtccattt cgtctcctca tctgcctcgg
     3721 cggggcgggc cggatcgacc gcccggatca ggggcaagga tagccgatcc cctccgtcag
     3781 cttgccgttc gccttcaatg tcataatctg cgggcttcgt atccccccac ctgccatgcc
     3841 actgacctcc ctcggcccgc tcatcgtccc gctcatcgtt gcctgcgcgc tgctcatgga
     3901 gaatgtggat tcgaccatcc tggtcacggc catccccgtg ctggcgcgcg acctgggcca
     3961 cgacccggtc accctcaagc tggccgtcac cagctatgtg gtgggcctgg gggtgttcat
     4021 ccccatctgc ggctgggtgg ccgacaagtt cggcccgcgc acggtctttc gcacggccat
     4081 cgccaccttc gtcatcgggt cgctgctgtg cgcggccgcc acctcgctga cgacgctggt
     4141 gctggcgcgc ttcatccagg gcgtgggcgg ggcgatgatg gtgccggtgg gccgcatcct
     4201 gatgttccgc gccgtgccca agtcggactt catccgcgcc gtcacctacc tgaccattcc
     4261 cgcccagctg gggccggtgc tggggccgct gctgggcggc ttcatcacca cctacctcaa
     4321 ctggcgcctg atgttcttca tcaacgtgcc catcggcatc ctgggcatct acctcaccaa
     4381 ccgctatatc gagaacaacc gcgaggaaga ccccggcaag ctggactgga cgggcttttt
     4441 cctgtccggc gcgggcgcgt cgctcttcat gctgggcatg tccatgatag gcagcggcct
     4501 catccaggaa ggcacggccg tggccctgac cctggccggc ttgctgatac tgggcatcta
     4561 ctggatctac tcgcagcgcg tggagaaccc gctgctggag ctgcgcttcc tgcacatccc
     4621 gaccttccag tgcagcgtgc tgggcggctc gctgttccgc atcgggctgg gcgccatgcc
     4681 cttcctgctg ccgctggccc tgcaggacgg cctgggcatg acggccttcg agggcggctt
     4741 catcacctgc tgctcggcca tcggcagctt cggcatcaag accatcgcca tcaaggtgct
     4801 caagcgctgg ggctttcgca acgtgctgat ggtcaacgcc gtgctggtgg gcatctccgt
     4861 ggcggtgtgc ggcgtgctgc acccgggcat gtcgcgcctg accatctggc tcatcgtgac
     4921 cctgggcggg ctgttccccg cgctgcagtt caccagcctg aactcgctgg cctacgccga
     4981 catcgaaagc cgcgacatcg gccgcgccac cagcgtggcc agcgtgatcc agcagatgtc
     5041 gctgggcctg ggcgtgaccg tcgccggcat gctgctgcat ttttcgcagg cgctgcacgg
     5101 ccggccggag atcgactgga ccgacttctg gcccgccttc ctggtggtgg gcatgttctc
     5161 cgtcgcctcg gtggcgttca cccggcgcat gccgcccgac ggcggcagcg gcgtgaccgg
     5221 gcgctagcct cgccccggcc gcgccggggc ccctggattt cccgcgcttg aaatttaaag
     5281 ctttaagctt aaactttatg gcacacggga cacgtccccg gaacaaagga tagccatgac
     5341 cacgacctcc gcccccttgc atgggcaaaa agccctggta accggcggca gcaccggcat
     5401 cggcgccgcg ctgtgcgaac gcctgatcga tctgggcgcc gaagtcgtgt cgctgtcgct
     5461 ggccgaccgc gccgacttca gccatccccg gttcacccag cgcgccgtcg acctgagcca
     5521 tccggccgcc gtcaaggccg ccgccgcgcg aatcgcccag gaaggcgact acgacatcct
     5581 ggtcaacaac gccggccgcg tcatccacaa gccgctggaa gaagtcaccg accaggattt
     5641 cgacgacctc atgaacctgc acgtgcgcgc cgccatcctg ctgacccagg ccgtcgcgcc
     5701 cggcatgaaa cagcgccgtt acggacgcat cgtcaacctc gccacgcgcg ccatcgtcgg
     5761 cctgcccgga cgcaccatct actcggcatc caaggccgcc atcgtgtcga tggaccgcac
     5821 ctgggccctg gagctcgccc cttacggcat caccgtcaac accatctcgc ccggtccgat
     5881 cggcacgccc atgctcaacg aaggcctgcc cgagggcgat cccaagcgcg atgccctggc
     5941 ggcgtcggtc ccgctcaagc gcctgggcac ggccgaagac gtggcgcgcg ccatcgcctt
     6001 cttcatcgac ccggccagca gctggatcac cgggcagaat ctgttcgtgt gcggcggctc
     6061 cagcatcgcg gcgtcgctcg ccctctaaca acaacccaac caccgcgcgt cgcgcccaag
     6121 gggtccacca ccatggccat tacctggttc tgcgtctggc aaggcaaagg cggcaccgcc
     6181 ggcgcccgcg tcgacgaata cgatctcaac gccctgaagg cgctggtgcg cggttgcccc
     6241 ggcttgctgg ccgggcacct cctcaccccg gcccaggcgc atgatccgca ctatgccggc
     6301 gaacaggagc aggcccagcc cgcgctcatc ctgcaactgg attttgccga tgccctggcg
     6361 ctggaaacgc tgctgcggcc cggcgcccat ctatcggtgc tggccgagcc caaattcctg
     6421 cccagcctca aggatgtccc ggccgcgcag caggccatgc tggcgcgccg ctacgccgtg
     6481 gccgaccggc agatcgcctc gcccgacggc agcagcctga gctactgggt ggagtacgcc
     6541 ggcccggccc aggacgaaaa cgcctggcat gccttctacg tcacccacca cccttatctg
     6601 ctggcaaagt tcccgggcat acgcgccatc gacatctaca cgccggccgt ggccatctgc
     6661 gaactgccgc tgcccgtgcg cccctgcctg cagcgcaaca aggtggtctt cgacaccccc
     6721 gaggccatga cggccgccat gcagacgccg gtgcgcgatg ccttgcgcgc cgacttccac
     6781 gagtttccgg ccttcacggg acatgcgctg catttcccgt tcaagacctg gacgtaccgg
     6841 ccctgagggc cgagtcaggc cggatcgggt cagatcacat tgaacttgtg cgtgccgtcg
     6901 cgcccgagct gttccaccag gccgaattcc cagtccaggt aggcctgcat ggcttgccgg
     6961 ggcgcatccg tgccttcgta cggacggcgg tagcgatcga tgcgcggcga tgccaggcgg
     7021 gtctcgccgc tttcgacggc gtggccggcc gtgatccagg cggcgttgcc gccgtccagc
     7081 acatacaccg gcacatcggt cagggcgcgg gcctcccgcg cggcgaagta cgccagcagg
     7141 ctcgagccgc acgtgaacac atagcgccgc ggcctgggcg ccgcccgcaa ggcctgcttc
     7201 aattgcgcgc gcagcgcgaa ccaggcgccc gggatatgcg ccttgatata ccgcacgccc
     7261 ggcgcgaaat cgagcacctg cgtcgagtcg cctgattcga gccattcggc cagcgtcgcc
     7321 acggagacgg tctcggccgc cggcggctcg ggcacggcgc gcttccaggc gcccggcaca
     7381 tgggccgcct tgtccggcgc ggggtcgacg gcataaacct cccagcccat ctgggccagc
     7441 cacgaagcgc tcatcgaggc ccgcacgccg tcgtcgtcgg ccagcacgat gcgcgcgccg
     7501 cgcaccggcg cgttgtggtc ggtctcctgc accagctggc cgcccggcgc cggcgcgaag
     7561 cccggcagat ggcctgcctc gtactcttcg ggcgtgcgca cgtcgaagca atacaaggtg
     7621 cgatcggcgg cgcgcaggct gtcgagcgcc tcgaatgcga tgcggcgcac gccggcgcgg
     7681 tcggccacg
//
LOCUS       DASWPO010000253        19717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1311076, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000253 DASWPO010000000
VERSION     DASWPO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 19717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19717
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311076"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VGC69_13300"
     CDS             complement(<1..830)
                     /locus_tag="VGC69_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY0296299.1"
                     /translation="MSTEFLDRINEPADLRSLDRRDLRRVADELRGFVLDSVSRTGGH
                     LSSNLGTVELALALHYVFDTPHDRIVWDVGHQSYPHKILTGRRDAMASLRQQGGISGF
                     PKRSESEHDAFGTAHSSTSISAALGMAVASRNAGIARQHIAVIGDGAMTAGMAFEAMN
                     NAGVTPNVNLLVILNDNDMSISPPVGALNRYLARLMSGRFYATARNVGRAVLSHVPPV
                     LELARRFEEHAKGMVSPATLFEEFGFHYVGPIDGHDLDALIPTLQNLKELQGLQFLHV
                     V"
     gene            complement(836..1747)
                     /locus_tag="VGC69_13305"
     CDS             complement(836..1747)
                     /locus_tag="VGC69_13305"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY0296300.1"
                     /translation="MPASQVGQSFPDWLHERVAHIEQVLDELLPAAQQVPQRLHEAIR
                     YAALGGGKRVRAAMVYAAGQAAGCGTPAADASLDRAAAAIELIHAYSLVHDDLPCMDD
                     DTLRRGRPTVHVQFDEATAMLAGDAMQPLAFELLADMPTSPALSVQAIAVLAHAAGSL
                     GMAGGQAIDLVSVGRQLTRDELQTMHSMKTGAMLVASVALGGIVAGASSGTRQALDAY
                     AQAIGLAFQVVDDILDVTADTASLGKTAGKDAADHKPTYVSLLGLDEARSYARQLGEA
                     AHEAIVPLGEGATRLAQLADFIVFRDR"
     gene            complement(1761..2042)
                     /locus_tag="VGC69_13310"
     CDS             complement(1761..2042)
                     /locus_tag="VGC69_13310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY0296301.1"
                     /translation="MTGQPEGGQPEGQSGKPGGPLPQEFEAALAELEALVAAMEDGSM
                     PLEQSLAAYRRGVELTRVCQEKLAQAEQQVKVLEGDLLRPLDSSALGDE"
     gene            2230..3390
                     /locus_tag="VGC69_13315"
     CDS             2230..3390
                     /locus_tag="VGC69_13315"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0296302.1"
                     /translation="MTDIGRMAHVVPARSPLAVSTYFDEALFAREQERIFKQSSLYIG
                     HEKLVPEVGDWRALIQENNGRALVRNALGPQGVELVSNVCRHRQSLMLGGAPGNVAGN
                     VNTHGNLSATGGNIVCPLHRWTYNKHGELLGAPQFDTTPCLNLERYTLRDCHGLLFEG
                     PRDPAADMAPLFARPEFDLGDYVLDHIEVHQCHYNWKTFIEVYLEDYHVGPFHPGLGR
                     FVTCDDLSWEFGDWYSAQRVGVHKALAQPGSEVYRTWHDKVLAFRNGQAPDFGAVWVT
                     YFPTHMIELYPHVLVLSTLYPKSPQETVNVVEFYYPEEIVAFEREFVEAQRAAYMETA
                     VEDDEIAERMDAGRRALMERGVSESGPYQSPMEDGMQHFHEWYRGIMDGPGA"
     gene            complement(3599..4462)
                     /locus_tag="VGC69_13320"
     CDS             complement(3599..4462)
                     /locus_tag="VGC69_13320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY0296303.1"
                     /translation="MTTTLITVSDLAARLAAGEADKGALRVFDLRHDLADHSVGRRAF
                     EQGHIPGARFLDHETELCAPRTGKNGRHPLPAPEHLAGLMAAHGVTPDTLVVAYDATG
                     GQYAAHLWWMLRWIGHERVAVLDGGWPAWSAAGLQTQAGPVQTLAPLAGAKPGASLVG
                     SVDAAAVLANIDKQDFVVVDARAPNRYRGEVEPMDPVAGHIPGALNRPNTQNVEADGR
                     FKSPAQLRAEFEAVLGGRAPAEVVHQCGSGITASHNLLAMEVAGLAGSRLYPGSWSEW
                     CADPARPVAKG"
     gene            complement(4567..5421)
                     /locus_tag="VGC69_13325"
     CDS             complement(4567..5421)
                     /locus_tag="VGC69_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0296304.1"
                     /translation="MQALWMLLASAMYAVMGALVKLSGEHGATLTEVVLFRGLPSVLL
                     LLFWARANHRSVITQTWRLHVWRNMAGVTSMWLGFYAISHLPLATATSLNYTSPLFIA
                     CWMLGWGGAQRDRVRIAAVALGFLGVIVVLRPSITADQWLPAMLGLVAGVMTAIAMMQ
                     IRQLGRVGEPEWRTVLFFSVAVCLSSCVGLLLEGWGRADWVGYASLVGVGLVGLLVQL
                     AQTRAFGKGAPLLTAALQYSTIIFAMVIGILFWRDAPDALAWAGSGFIILAGLISVWR
                     TARQAAPA"
     gene            5559..6443
                     /gene="htpX"
                     /locus_tag="VGC69_13330"
     CDS             5559..6443
                     /gene="htpX"
                     /locus_tag="VGC69_13330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY0296305.1"
                     /translation="MKRIFLFLATNLAVMLVLSATLNILGVNRYLTAQGLNLPMLLAF
                     SAVVGFAGALISLFMSKPMAKWSTGAQVIDPGAPRSQREAWLVDTVHQLADRAGIGRP
                     EVAVYDGAPNAFATGAFKNDALVAVSTGLLESMSEAEVTAVLGHEISHVANGDMVTLT
                     LIQGVINTFVVFLARVVGYVIDRTVLRNDRSGSGVGYYITVMVCELVFGLAASIIVAW
                     FSRQREFRADAGSARLLGSREPMIHALARLGGLQAGELPKSFAASGIAGAGGLGALFA
                     SHPPLEARIAALQRMRQA"
     gene            complement(6453..8105)
                     /locus_tag="VGC69_13335"
     CDS             complement(6453..8105)
                     /locus_tag="VGC69_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296306.1"
                     /translation="MRRIDVPLPGRLFSLCGVWAVLILGVLAWSIVRKTGGFWYTLDD
                     PYIHLRLSEVIRGGGYGINPGIPAAPSSSILFPFLLAFAARTAFHPYVPFFLNGAATL
                     AALWGLCRIVAPGRAQRAGEIRRQALFLVVASFFGGLFGVVFTGLEHSLHIALCLGIA
                     LGLVRALRNGRTDGWLVALIVLCPLVRFEGAVLSCVALTVLLAERQYRPAAYGILALV
                     LAAGAWAGFMHGLGLPLLPSSVLVKSQLAAQSVGGMRHVGKIAWDGLRAALAEPLGLM
                     TIALSLGTVMYMAGIAVRQAGRARRMALMWAYLISVVVAAHVVFSFRGWNGRYVVYIN
                     MLCVYAMAWALFSEEARAARDSASSAVKGAAVALLDRRLVAAWVLLYCFATSPQVLGT
                     LMTPLAADSIRLQQGQMARFAHDWLRAPVAINDLGLVSYRSQAYVLDLWGLGSDEARR
                     FRQASPQDAQWMSQLAATHGVPLAMLYESWFPVLPQTWCKLGDLRLDVPRVAPADSRV
                     AFYATAPGEAVGLRRQLMDFSRTLPAGAAFVFTGAAPGACPV"
     gene            complement(8140..9123)
                     /locus_tag="VGC69_13340"
     CDS             complement(8140..9123)
                     /locus_tag="VGC69_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY0296307.1"
                     /translation="MARIVKAIRIEQHGGPEVLKLVDVELTPPAPGEVTIRQHAVGLN
                     FIDIYYRSGLYPHPLPHGLGAEGAGVVEAVGSGVKHLKVGDRVAYGQSPLGAYAEVRN
                     VPVASLVKLPKGIEFDAAAAMMLKGLTVQYLFRQTYRLQGGETILFHAAAGGVGLIAC
                     QWAKALGVKLIGTVSSPEKAELARANGAWKTIDYSRENVAEHVLELTGGKKVPVVYDG
                     VGKDTWEASLDSLEPRGLMVSFGNASGPVTGVNVGILNQKGCLYLTRPSLGVHVNTPE
                     KLKAAADELFGLVLKKKINIHIAQRYALAQAGEAQTALAARKTTGATALMV"
     gene            complement(9194..10162)
                     /locus_tag="VGC69_13345"
     CDS             complement(9194..10162)
                     /locus_tag="VGC69_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0296308.1"
                     /translation="MNHTVATAAPAPASLAPSSSQRLTGILFACVGAILFAGKAVVVK
                     LTYRYGIDSVTLIALRMLFSAPFFLAVAWWQAGRARRGELPALTRREGVLIGILGLMG
                     YYLSSFLDFLGLQYVSAGLERLVLFLAPSMVVLISAWWFKRPVSRRQWFSMLVCYAGV
                     VLVFAQDLVGKGVGDGNLWLGSLYVFGAALSYAVYLSGTGELVKRVGATRLVAYAMLV
                     SCLACVIQFFCVHPAALLLQQPAGVYGYSLVHAVFNTVLPVFMLMWAVARIGAPTASL
                     LGMVGPVSVLFLAAWLLGEPITWWQLLGTVLVLSGVFVLSSRRSRA"
     gene            10202..11065
                     /locus_tag="VGC69_13350"
     CDS             10202..11065
                     /locus_tag="VGC69_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0296309.1"
                     /translation="MTHQRALLYVHIVAVCFGLAGVFGHLVSAGATVITLGRALCAVL
                     TLVCVGRLQRRPFLGVLTPAQLFVLVVSGCLLAAHWVTFFLAVKTGGIAVATLGFASF
                     PAFTTLLEALLPRERVSKAEWMALALVTVGLILVTPSFDLADQGTIGLGWGLLSGLSF
                     ALLALSNRRGTVGMDPIQVACWQNIVVTLVMLPAAASQLAGATAWDWLWLAMLGVFCT
                     GLAHFLFVSSLAGIKASTAGLVIALEPVYGITFAWALFGQQPTLRMLAGGALVIAAIV
                     WSGLRRPAAEA"
     gene            11141..11644
                     /locus_tag="VGC69_13355"
     CDS             11141..11644
                     /locus_tag="VGC69_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0296310.1"
                     /translation="MNAPRPPHNDKPHYSGDEGCLSIDNAGFLIKMVHLSLNRALDQQ
                     MAMIDLTAMQWRPLVMLSRGIADTPAELARLAGVDTGAMTRTLDRLEAKGLLRRNRSQ
                     EDRRVIKLELTESGKEKGRQIPPHVAKVLNQHLRGFSDEEVIQLRHMMRRMLANGSFD
                     LPSGLPE"
     gene            11759..13195
                     /locus_tag="VGC69_13360"
     CDS             11759..13195
                     /locus_tag="VGC69_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0296311.1"
                     /translation="MKRLLLTTLVLALSGCALMEPSAPPAAQLDGARLGLAEQPVNWP
                     DAQWWRRYGDTQLDQLVQEALAGNPTLAAAQARLAQANAAISRARAPLLPQVDADYQL
                     TREHMPLDYIYPPPFGGNNYSDNRLALDFHYELDFWGKNRDLLHAAVAQRDAASADAQ
                     AARNLLAHSVVHSYLNLQNALAQHEVLEQTAKQRKELVDLTHGRQKSGLDTMVEVRQA
                     ESALASVQVDTTQEETTIAQLRNQIAALLGAGPQRGQALARVQLTVPSGVLPAAVPLE
                     LLGRRPDVAAARWRAEAARSQIGAAKADFYPNVNLTAFAGFQALGPLSTLFDGGKTVG
                     IGPAITLPIFHGGELNAQLAGRRAEADVAVADYNNTVLDAVRQTADAIDALRLIEREK
                     QEQHQARTAIEDAYKLAVDRYKAGLGNYLSVLIAQNSVLAQARLDTDLRIRAYQLDAD
                     LAYAMGGGYQDPDQAAAQAARTEDKTSD"
     gene            13221..14411
                     /locus_tag="VGC69_13365"
     CDS             13221..14411
                     /locus_tag="VGC69_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0296312.1"
                     /translation="MTETATPLPNPNRTRLLVTAAVIFVLLGVAYAVWWFLYGSHFEE
                     TDDAYVHGNLVQITPQIAGTVVSIDTDDTRSVKAGQALVRIDSSDADVALAQAQAQLA
                     QTVRQVRTRYVQNDALAADVEVSQAEIVRAQSDLARAQSDLKRRQDLARSGGVSGEEL
                     LHADSAVKTAQSSLAQAKASLAASQAHLETNLALTHGTTVEQHPDVLNAAAAVRSAWL
                     AKSRTVLPAPVDGVVARRSVQVGQRVAPGAVLMNVVPLDQVWIEANFKEGQLSKMQLG
                     QPATIKSDLYGSSVTYHGTVIGMDAGTGSAFALLPAQNATGNWIKVVQRLPVRIAIDP
                     ADLKQHPLRVGLSTDVEVDTSAAGDAAHAEQSKAALLSTPVFDPSHGEADAMIAGIIK
                     ANLQ"
     gene            14419..16032
                     /locus_tag="VGC69_13370"
     CDS             14419..16032
                     /locus_tag="VGC69_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY0296313.1"
                     /translation="MSDIQDGTPAQPAAPAGQPTPRTYPPLKGGKLVLGAVALSTAVF
                     MNVLDTSIANVSIPTISGNLGVSSSQGTWVITSFAVANAITVPLTGWLTQRFGQVRLF
                     LISTLLFVLASWLCGFSPSLEALIAFRVLQGAVAGPMIPLSQTLMLASFPKEKSGMAL
                     AFWAMTTLVAPVAGPLLGGWISDNYSWPWIFYINVPVGLLAAWVSWRIYRDRESVTHK
                     LPIDKIGLGLLVVWVGCLQIMLDKGKELDWFESSTIVILAATVIIAFVFFLIWELTDA
                     HPVVDLRLFKVRNFTVGAITLAVAYGIFFGNVVLLPLWLQSDMGYTATYAGMVTAPVG
                     ILAIILTPIVGKLLARFDPRLIVTVSFLIFAAVSWMRAGFNTDTDVGTLMIPTVIQGA
                     AMAGFFVPLTAITLSQIEPWRIPAASGLSNFLRLTAGGFGTSIATTLWENRASMHHAQ
                     LTEIAKPGQQAFDQTMSTLQSGGYSQTQSLSMIDRIVDLQAFTMSVTDIFFASAMVFL
                     LLTGLVWFSRTRKAGKGGGGAAEAAAGAH"
     gene            16139..16429
                     /locus_tag="VGC69_13375"
     CDS             16139..16429
                     /locus_tag="VGC69_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0296314.1"
                     /translation="MSRPAAIPTVQIDNEHVTVTEWRFPPGGETGWHRHGMHYVVVPQ
                     TTGELLLETPQGEMRSQLTTGVSYTRPVGVEHNVVNPNDTEFVFVEIELKKA"
     gene            16451..17713
                     /locus_tag="VGC69_13380"
     CDS             16451..17713
                     /locus_tag="VGC69_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEY0296315.1"
                     /translation="MSSIADIKINGQRLWQSLMDLARIGATEKGGNCRLALTDLDRQG
                     RDLVVGWMREAGLEITVDQIGNIFGRRPGKNPALPSIATGSHIDTQPTGGRFDGCFGV
                     LAGLEVMRTLQEQGIATEAPLELAIWTNEEGSRFVPVMMGSGVFCHIFSLETALSATD
                     IDGKSVRDELEKIGYAGDAPVGARQFGKYFEAHIEQGPVLEAEDKVIGVVTGSLGVLW
                     YDCTVTGMEAHAGPTPMSLRRDALYASTFIMQKVVEIALEYAPQGRGTVGAAYVHPCS
                     RNVIPGSVTFTIDIRHETAAKLAEMDAKLRAAIQEVADGKTTGHAMQVELKPSSHFPP
                     TPFAADMVASVREQARARGYAEMDIVTGAGHDAVHVATVSPTAMIFVPCKDGISHNEV
                     EDADPAHLEAGANVLLGAMLTHAGLAGR"
     gene            18000..19370
                     /locus_tag="VGC69_13385"
     CDS             18000..19370
                     /locus_tag="VGC69_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0296316.1"
                     /translation="MTTFSKAQQQDVQYHLHPYTNGVKHRESGPRIITRGEGIYVYDD
                     QGNRYLESMAGLWSVAVGFGEPRLVEAATRQLQALPYYHTFSHKSHPSAIELAERLVG
                     YTHGKMAQAFFTNSGSEANDTVVKMVWYYNNALGRRDKKKIIARLGSYHGVTVASASL
                     TGLPGNHRDFDLPLPQIKHTANPHYYRYGKAGETEEAFATRLAQELEDLILREGPDSV
                     AAFIGEPVMGAGGVIIPPKTYWEKIQAVCRKYDLLIVADEVITGFGRLGKRFGTEVYD
                     IDADIQVLSKQITSSYQPLGAVLINAKVAETINQNSGKLGSFGHGYTASGHPVATAVA
                     LENLKIIDERGLIDNAAEMGKLLRSELDAKLAGHPLVGEIRSQGLIAAVEMVADRATR
                     KQFDPVGKAGGYAYERAHSHGIIVRGIMDNVAVCPPLIINAEQVRDMVDRVARTVDDT
                     AAYLRG"
BASE COUNT         3486 a         6950 c         6282 g         2999 t
ORIGIN      
        1 accacgtgca ggaactgcag gccctgcagt tccttgaggt tttgcagggt ggggatgagc
       61 gcgtcgaggt cgtggccgtc gatcgggccc acgtagtgga agccgaactc ttcgaacagc
      121 gtggccggcg acaccatgcc cttggcgtgc tcctcgaagc ggcgcgccag ctcgagcacg
      181 ggcggcacgt gcgacagcac cgcgcggccg acattgcgcg cggtggcata gaaccggccg
      241 gacatcagcc gcgccaggta gcggttgagc gcccccaccg gcggcgagat cgacatgtcg
      301 ttgtcgttga ggatgaccag caggttcaca ttgggcgtga cgccggcgtt gttcatggcc
      361 tcgaaggcca tgccggccgt catggcacca tcgccgatca cggcgatgtg ctggcgggcg
      421 atgccggcgt tgcgcgatgc caccgccatg cccagcgcgg ccgagatcga ggtggacgag
      481 tgcgccgtgc cgaaggcgtc gtgttcggat tcgctgcgct tgggaaagcc cgagatgccg
      541 ccctgctgcc gcaggctggc catggcgtcg cggcggccgg tgaggatctt gtgcggatac
      601 gactggtggc ccacgtccca cacgatgcga tcgtgcggcg tgtcgaacac ataatgcagg
      661 gccagcgcga gctcgaccgt gcccaggttg gacgacagat ggccgccggt gcgcgagacc
      721 gaatccagca cgaatccgcg caactcatcg gccacgcgcc gcaaatcgcg gcggtccagg
      781 ctgcgtagat cggcgggttc gttgatgcga tccaggaatt cggtgctcat tgctttcagc
      841 ggtctcggaa aacgatgaaa tcggccagtt gcgccaggcg cgtggcgccc tcgcccagcg
      901 gcacgatggc ctcgtgcgcc gcctcgccca gttggcgggc gtacgagcgg gcctcgtcca
      961 gacccagcag cgacacatag gtaggtttgt ggtcggcggc gtccttgccc gccgtcttgc
     1021 ccaggctggc cgtgtcggcg gtgacgtcga ggatgtcgtc gaccacctgg aaggccaggc
     1081 cgatggcttg cgcataggcg tcgagcgcct ggcgcgtgcc ggagctggcg ccggccacga
     1141 tgccgcccag cgcgacgctg gcgaccagca tggcgccggt cttcatgctg tgcatggttt
     1201 gcagttcgtc gcgcgtgagc tggcgcccca cgctgaccag gtcgatggct tgcccacccg
     1261 ccatgcccag gctgcccgcg gcatgcgcca ggacggcgat ggcctgcacc gacagggccg
     1321 gcgaggtggg catgtccgcc agcagctcga aggccaacgg ctgcatggca tcgcccgcca
     1381 gcatggcggt ggcctcgtcg aactgcacgt gcaccgtggg ccggccgcgg cgcagcgtgt
     1441 cgtcgtccat gcagggcagg tcgtcgtgca ccagcgagta ggcgtgaatc agttcgatgg
     1501 cggcggcggc gcggtcgagc gaggcgtccg cggccggcgt cccgcagccc gcggcctgcc
     1561 cggccgcgta caccatcgcc gcccgcaccc gcttgccgcc gcccagcgcc gcgtagcgga
     1621 tggcttcgtg caggcgctgc ggcacctgtt gcgcggcggg cagcagctcg tcgagcacct
     1681 gttcaatgtg cgcaacgcgc tcatgcagcc agtctggaaa ggactggccg acttgggatg
     1741 caggcatgga tgtcatctct ttattcatct cccagggccg acgagtcgag cggccgcagg
     1801 aggtcgccct cgagcacctt gacctgctgt tcggcctggg cgagcttttc ctggcaaacg
     1861 cgcgtgagtt cgacgccgcg gcgataggcc gcgagcgatt gctcgagcgg catggaacca
     1921 tcttccatgg cagccaccaa ggcttcgagt tcggccaggg ccgcctcaaa ctcctggggc
     1981 aaggggccgc cgggtttgcc ggactggccc tcgggttgac cgccttcggg ttgaccggtc
     2041 actgcggctt gtttcgaggc caagcgagtc tccagacttg ggggaaataa tcttgggatt
     2101 gtaagggaaa gacccgggaa aaggccgagc attgcaaagg atcttcaggt aaactcctgc
     2161 cccttaccgc cagatacgcg tgcgccgtgt cccgttctgg ctttttctag tctttagcgg
     2221 agaaccttca tgaccgacat tggtcgcatg gcacacgtcg tgccagctcg cagcccgctt
     2281 gccgtcagca cgtatttcga cgaagccctg ttcgcccgcg agcaagaacg cattttcaag
     2341 cagtcatcgc tgtacatcgg ccacgagaag ctcgtgcctg aagtcggcga ctggcgtgcc
     2401 ctcatccagg aaaacaacgg ccgcgcgctg gtgcgcaatg ccttggggcc gcaaggcgtc
     2461 gagctcgtct cgaacgtctg ccgccaccgc cagtccctga tgctgggcgg cgccccgggc
     2521 aatgtggcgg gcaacgtcaa cacccacggc aacctgtcgg ccacgggcgg caacatcgtc
     2581 tgtcccctgc accgctggac gtacaacaag cacggcgagc tgctgggcgc cccgcagttc
     2641 gacaccacgc cctgcctgaa cctcgagcgc tacaccttgc gcgactgcca cggcctgctg
     2701 ttcgaaggcc cgcgcgatcc ggccgccgac atggcgccgc tgttcgccag gcccgagttc
     2761 gatttgggcg actacgtgct cgaccacatc gaggtccacc agtgccacta caactggaag
     2821 accttcatcg aggtctacct cgaggactac cacgtggggc ccttccatcc gggcctgggc
     2881 cgcttcgtca cctgcgacga cctctcctgg gagttcggcg actggtacag cgcgcagcgt
     2941 gtgggcgtgc acaaggcttt ggcgcagccc ggctccgagg tgtaccggac ctggcacgac
     3001 aaggtgctgg cgttccgcaa cggccaggcg ccggatttcg gcgcggtgtg ggtcacctac
     3061 tttcccacgc acatgatcga gctctatccg cacgtgctgg tgctgtcgac gctttacccc
     3121 aagagcccgc aggaaacggt caacgtggtc gagttctact accccgagga aatcgtcgct
     3181 ttcgagcgcg agttcgtcga ggcgcagcgc gcagcctata tggagacggc cgtcgaggac
     3241 gacgaaatcg ccgagcgcat ggacgccggc cgccgtgccc tgatggaacg cggcgtgagc
     3301 gagagcgggc cctaccagtc gcccatggaa gatggcatgc agcatttcca cgagtggtat
     3361 cgcgggatca tggatgggcc gggggcttga tggccgtctt gccttgttga tgcgccgtct
     3421 ttgagttctt gagacgccga ggctgccctg cggaattccg atgctcgccc ccactggggg
     3481 ctgcgcttcg gattcctccg ggatcctcgg ctcaaggcac ggcaaattgc agcgataaaa
     3541 aggcctcgta atccgtggct ttttattgtt gcatgagagc ggtcgggggc cttgctccct
     3601 agcccttggc cacgggccgc gccggatcgg cgcaccactc gctccacgag ccggggtaca
     3661 agcgcgagcc cgccaggccg gccacttcca tcgccagcag gttgtggctg gcggtgatgc
     3721 ccgagccgca ctggtgcacc acctccgcag gcgcgcggcc gcccagcacg gcctcgaatt
     3781 cggcgcgcag ctgcgcgggc gacttgaaac ggccgtcggc ttcgacgttt tgcgtgttgg
     3841 gacggttgag cgcgccgggg atgtggcccg cgaccgggtc catgggttcg acctcgccgc
     3901 gatagcggtt gggcgcgcgg gcgtcgacca cgacgaaatc ctgcttgtcg atgttggcga
     3961 gcacggccgc cgcgtcgacg ctgccgacca gcgatgctcc cggcttcgcg ccggccaacg
     4021 gcgccagcgt ttgcacggga ccggcctgcg tttgcagccc ggccgccgac caggcgggcc
     4081 agccgccgtc gagcacggcc acgcgctcgt ggcctatcca gcgcagcatc caccacaggt
     4141 gggcggcata ctggccgccg gtggcgtcat aggccacgac cagggtgtcg ggcgtgacgc
     4201 cgtgcgcggc catcaggccg gccaggtgct cgggcgcggg cagcgggtgc cggccgttct
     4261 tgcccgtgcg cggcgcgcaa agctcggttt cgtggtcgag aaagcgcgcg cccggaatat
     4321 ggccctgctc gaaggcgcgg cgccccacgg aatggtcggc caggtcgtgg cgcaggtcga
     4381 agacgcgcag cgcgcccttg tcggcctcgc cggcggccag gcgcgcggcc aggtcggaga
     4441 cggtgatcag ggtggtcgtc atggatgttt cctcctagag tcgccgcgtc ggggcgcggc
     4501 ctatcggtct gtcttgcggg ggctttgcag taacggttgg tttgcgggca acggcgttgc
     4561 aagggatcag gccggcgcag cctgccgcgc cgtgcgccag accgagatca gtccggccag
     4621 gatgatgaac ccgctgcccg cccaggccag cgcatcgggc gcgtcgcgcc agaacaggat
     4681 gccgatgacc atggcgaaaa taatcgtgga atattgcagg gccgcggtca acagcggcgc
     4741 gcccttgccg aaggcgcggg tctgcgccag ctgcaccagc aggccgacca gccccacccc
     4801 gaccagcgac gcatagccca cccagtcggc ccggccccag ccttccagca agaggcccac
     4861 gcagctgctc aggcagaccg cgaccgagaa gaacagcacc gtgcgccatt ccggctcgcc
     4921 cacccggccg agctggcgta tctgcatcat ggcgatggcc gtcatcacgc ccgccaccag
     4981 gcccagcatg gcgggcagcc actggtcggc cgtgatgctg ggacgcagca ccacgatcac
     5041 gcccagaaag cccagggcca cggcggcgat gcgcacccgg tcgcgctgcg cgccgcccca
     5101 gcccagcatc cagcaggcga tgaagagcgg cgaggtgtaa ttcaggctcg tcgccgtggc
     5161 cagcgggaga tgcgaaatgg cgtagaagcc cagccacatc gacgtgaccc cggccatatt
     5221 gcgccacacg tgcaggcgcc aggtctgcgt gatgacggac cggtggttgg cccgcgccca
     5281 gaagagcagc agcagcaccg aaggcaggcc gcggaacagc acgacttccg tcagcgtggc
     5341 gccgtgctct ccggacaact tcacgagcgc ccccatgacc gcatacatgg cagacgccaa
     5401 caacatccag agagcctgca tgggagctac ggggccgaag tcgattgaaa gaagtcgcta
     5461 atatactccc ttgcccataa attcagtttt cagtaagaat agggattgcc gctacgcgcg
     5521 gcgtataaaa cacactgcag gacatcgacg cacaaaccat gaaacgcatc ttcctcttcc
     5581 tggccaccaa cctcgccgtg atgctggtgc tgtcggccac gctgaacatc ctcggcgtca
     5641 accgctacct cacggcgcag ggactgaacc tgcccatgct gctggcgttc tcggccgtgg
     5701 tgggcttcgc cggcgcgctg atctcgctct tcatgagcaa gcccatggcc aagtggagca
     5761 ccggcgcgca ggtcatcgac cccggcgccc cgcgcagcca gcgggaagcc tggctggtcg
     5821 acaccgtgca ccagctggcc gaccgggccg gcatcggccg gcccgaagtg gccgtctacg
     5881 acggcgcgcc caacgccttt gccacgggcg cgttcaagaa cgacgccctg gtggccgtgt
     5941 ccaccggcct gctcgaaagc atgagcgagg cagaagtcac cgccgtgctg ggccacgaga
     6001 tctcgcacgt ggccaacggc gacatggtca cgctcaccct gatacagggc gtgatcaata
     6061 ctttcgtggt gttcctggcc cgcgtggtgg gctatgtcat cgaccgcacg gtgctgcgca
     6121 acgaccgcag cggcagcggc gtcggctact acatcaccgt gatggtctgc gaactggtgt
     6181 tcggcctggc agcctcgatc atcgtggcct ggttctcgcg ccagcgcgag ttccgcgccg
     6241 acgccggctc ggcgcgcctg ctgggctcgc gcgagcccat gatccacgcc ctggcccgcc
     6301 tgggcgggtt gcaggccggc gagctgccca agtcgttcgc ggcgtccggc atcgccggcg
     6361 cgggcggcct gggcgccctc ttcgcctcgc acccaccgct ggaagcgcgc attgcggcgc
     6421 tgcagcggat gcgacaggcc taagtaaacg gactacaccg ggcacgcgcc cggtgcagcg
     6481 ccggtgaaca caaaggcggc acccgctggc agcgtgcggg aaaaatccat caattgcctg
     6541 cgcaaaccga ccgcctcgcc cggcgctgtc gcatagaaag cgacgcgcga atcagccggc
     6601 gcgacgcgcg gcacgtccag ccgcagatcg cccagcttgc accacgtctg aggcaagacg
     6661 ggaaaccagc tctcatacaa catggccagg ggcacgccgt gcgttgccgc gagctgcgac
     6721 atccattgcg cgtcctgcgg cgaagcctgc cgaaagcgcc gggcttcgtc ggaacctagc
     6781 ccccacaagt ccagcacata tgcctgactg cggtacgaca ccaggccgag gtcattgatc
     6841 gcgaccggcg ctcgcaacca gtcgtgcgcg aaacgcgcca tctgcccttg ctgcaagcgg
     6901 atgctatccg ccgccaatgg agtcatgagc gttccaagca cctgcgggct ggtcgcgaag
     6961 caatacagca gcacccaggc ggcaacgagc ctcctgtcca gcaaggccac ggctgcgcct
     7021 tttacggcgg acgatgcgct gtcgcgcgcg gccctggcct cttcgctgaa cagagcccat
     7081 gccatggcat atacgcacag catattgatg tagaccacgt aacgcccgtt ccagccgcgg
     7141 aagctgaaga cgacgtgcgc cgcgacaaca acggatatca aatacgccca catgagcgcc
     7201 atgcgccgcg cgcggccagc ttggcgtacg gcaatgccgg ccatgtacat caccgtgccc
     7261 aggctcagcg cgatcgtcat caggccaagc ggctccgcca gcgccgcgcg caagccgtcc
     7321 cacgctatct tgccgacgtg ccgcatgcct cccaccgact gagcggccag ttgggatttc
     7381 accagcacgg acgatggcag gaggggcaac cccaagccat gcatgaaccc ggcccacgcg
     7441 ccagccgcca ggacgagggc caggatgccg tacgcggcag gccggtattg gcgttcggcc
     7501 aacaggacgg tcaatgccac gcaggacagg accgcgccct cgaaccggac caggggacac
     7561 aggacgatca gcgcgacgag ccagccgtcg gtgcggccgt tgcgaagcgc gcgcaccagg
     7621 cccagggcaa tgcccagaca cagcgcgata tgcagggaat gttctagacc cgtgaacact
     7681 acgccgaaca aaccgccgaa gaaagacgcc accaccagga acaaggcctg gcgccggatc
     7741 tcaccggcgc gctgcgcccg ccctggagcg acgatccggc acaatcccca caacgcagcc
     7801 agcgtggccg cgccattgag gaagaacggc acataagggt ggaaggccgt gcgcgcggcg
     7861 aacgcgagga gaaagggaaa caggatggaa gaactcgggg cggcggggat gccaggattg
     7921 atgccatacc ccccgccccg tatcacctcg ctcaggcgca gatgaatgta ggggtcgtcc
     7981 aaggtgtacc agaagccgcc cgtcttgcgg acgatgctcc aggcaaggac gccaaggatc
     8041 aggacagccc atactccgca gagagagaag aggcgcccag gcaacggcac atcgatacga
     8101 cgcactgcca ctatccttgt gcagcgacat gggctgcact tacaccatca gcgccgtcgc
     8161 gcccgtcgtc ttgcgcgcgg ccagcgcggt ctgcgcctcg ccggcctgcg ccagcgcata
     8221 gcgctgggcg atgtggatgt tgatcttctt cttgagcacc aggccgaaca gctcgtcggc
     8281 cgcggccttg agtttttcgg gggtgttcac gtgcacgccc agcgagggac gggtgagata
     8341 caggcagccc ttctggttga ggatgccgac gttcacgccc gtgaccggac cggaggcatt
     8401 gccgaagctc accatcaggc cgcgcggctc gaggctgtcc agcgaggctt cccaggtgtc
     8461 cttgcccacg ccgtcgtaaa ccaccggcac cttcttgccg ccggtgagct ccagcacgtg
     8521 ttcggccacg ttctcgcgcg aatagtcgat ggtcttccag gcgccgttgg cccgggccag
     8581 ctcggccttc tcggggctgg acaccgtgcc gatgagcttg acgcccaatg ccttggccca
     8641 ttggcaggcg atcaggccca cgccgcccgc ggccgcgtgg aacagaatgg tctcgccgcc
     8701 ctgcaggcgg taggtctggc ggaacaggta ctgcaccgtg aggcccttga gcatcatcgc
     8761 ggccgccgca tcgaactcga tgcccttggg cagcttgacc aggctggcca ccggcacgtt
     8821 gcgcacctcg gcatacgcgc ccagcgggct ctggccgtag gccacgcggt cgcccacctt
     8881 gagatgcttc acgccggaac ccacagcctc gaccacgccg gccccttccg cgcccaggcc
     8941 gtgcggcagc gggtgcgggt acaggcccga acggtagtag atgtcgatga aattcaggcc
     9001 gacggcgtgc tgccggatgg tgacctcgcc gggtgcgggc ggcgtcaact cgacgtcgac
     9061 gagcttgagc acctcgggac caccgtgctg ttcgatgcga attgccttga ctatgcgggc
     9121 catgcgggcc tcctctgatg aatgcggatc gataaagcgt ataaagccga aaacctgatg
     9181 cctgcccgcg ccgtcaagcc ctgcttcggc ggctcgacag gacgaacacc ccgctcagca
     9241 ccagcacggt acccagcagc tgccaccagg tgatcggctc acccagcagc caggccgcga
     9301 gaaacagcac cgataccggc cccaccatgc ccagcagcga tgccgtgggc gcgccgatgc
     9361 gcgccaccgc ccacatgagc atgaagaccg gcagcaccgt gttgaacacg gcgtggacga
     9421 gcgaataacc gtagacgccg gccggctgct gcagaagcaa ggcggcagga tgcacgcaga
     9481 agaactggat cacgcaggcc aggcacgaca ccagcatggc ataagcgacc aaccgcgtgg
     9541 cgcccacccg cttgaccagt tctcccgtgc cgctcaggta gacagcatag ctgagcgccg
     9601 cgccgaaaac atacaaagac cccagccaca gattgccgtc gccgacgccc ttgccgacga
     9661 ggtcctgcgc gaacaccagc accacgcccg catagcacac cagcatcgag aaccactgcc
     9721 gccgcgacac cgggcgcttg aaccaccagg ccgagatcag caccaccatg ctcggcgcca
     9781 ggaacagcac cagccgctcc agcccggccg acacgtactg caggcccagg aagtcgagaa
     9841 agctcgacag gtaatagccc atcaagccca gtatgccgat cagcacgcct tcacgccggg
     9901 tcagcgcagg cagctcgcca cgccgcgcgc gcccggcctg ccaccaggcc actgccagga
     9961 agaacggcgc cgaaaagagc atgcgcaagg cgatgagggt gaccgaatcg atgccgtagc
    10021 ggtaggtcag cttgaccacc accgccttgc cggcaaagag gatggcgccc acgcaggcga
    10081 agaggatgcc ggtcaatcgt tgggaagagg acggggcaag agaggccggc gctggcgcgg
    10141 ccgtggcaac ggtgtgattc atgcgatgat tttaagcatg actcgctccc gccccgctcc
    10201 gatcacccat caacgtgccc tgctctatgt tcatatcgtc gccgtgtgct tcggcctggc
    10261 cggggtcttc ggccacctgg tctcggccgg cgccacggtc atcacgctgg gccgggcgct
    10321 gtgcgcggtg ctgaccctgg tctgcgtcgg ccgcctgcag cgccgcccgt tcctgggcgt
    10381 gctgacgccc gcgcaactct tcgtgctggt cgtctccggc tgcctgcttg ccgcgcactg
    10441 ggtgaccttc ttcctggccg tcaaaacggg cggcatcgcc gtggccacgc tgggctttgc
    10501 cagcttcccg gccttcacca ccctgctcga ggccctgctg ccgcgcgagc gggtgagcaa
    10561 ggccgaatgg atggcgctgg cgctggtgac cgtgggcctg atcctggtga cgccgtcctt
    10621 cgacctggcc gaccagggca ccatcggcct gggctggggg ctgctgtcgg gcctgtcgtt
    10681 cgcgctgctg gccctgagca accgccgcgg caccgtcggc atggatccca tccaggtggc
    10741 ctgctggcag aacatcgtcg tcaccctggt catgctgccc gcggcagcct cgcagctggc
    10801 cggcgccacg gcctgggact ggctgtggct ggccatgctg ggcgtgttct gcaccgggct
    10861 ggcgcacttc ctgttcgtct ccagcctggc ggggatcaag gccagcacgg ccggcctggt
    10921 gatcgcgctc gaacccgtct acggcattac ctttgcctgg gcgctgttcg gccagcaacc
    10981 cacgctgcgc atgctggccg gcggcgcgct ggtgatcgcc gccattgtct ggtcggggct
    11041 gaggcggccg gcggccgaag cctgaggtgt ggctaattgt ccatctataa cagatagtgc
    11101 cttgacaata tctgcctaag caattaaatt ggcggacatc atgaacgccc cacgtccccc
    11161 gcacaacgac aagccgcatt acagcggcga cgagggttgc ctcagcatcg acaacgccgg
    11221 cttcctgatc aagatggtgc acttgtcgct gaaccgggcg ctcgaccagc aaatggccat
    11281 gatcgacctc accgccatgc aatggcgccc gcttgtcatg ctctcccgag gcatagccga
    11341 cacgccggcc gaactggccc gcctggccgg cgtcgacacc ggcgccatga cccgcacgct
    11401 ggaccgcctc gaggccaagg gcttgctgcg ccgcaaccgc agccaggaag accgccgcgt
    11461 catcaagctc gaactcaccg agagcggcaa ggagaaaggc cgccagatcc cgccccatgt
    11521 cgcaaaagtg ctcaaccagc acctgcgcgg cttttccgac gaagaggtca tccagctacg
    11581 gcacatgatg cgccgcatgc tggccaacgg ctccttcgac ctgccctccg gactgcctga
    11641 ataaccctat cttttcatac gacatgcgac gaatcgcaat attgttttga caaaattagc
    11701 tgcctagtca gatatagatc accatataaa tagctactcc acacttagta gaacgagcat
    11761 gaaacgctta ctccttacca ctctggtgct ggccctgagc ggctgcgccc tgatggagcc
    11821 ctccgccccg cccgcggccc aactcgacgg cgccagactg ggcctggccg agcaaccggt
    11881 gaactggccc gacgcgcaat ggtggcgccg ctacggcgat acgcagctgg accagctcgt
    11941 ccaggaagcc ctggccggca accccacgct ggcggccgcc caggcccgcc tggcccaggc
    12001 caatgccgcg atcagccgcg cccgcgcgcc cctcctgccc caggtcgatg ccgactacca
    12061 gctcacccgc gagcacatgc ccctggacta catctacccg ccccccttcg gcggcaacaa
    12121 ctacagcgac aaccgcctgg cgctggactt ccactacgag ctcgacttct ggggcaagaa
    12181 ccgcgacctg ctgcacgccg ccgtcgcgca gcgcgatgcc gcgtcggccg atgcccaggc
    12241 cgcccgcaac ctgctggccc actcggtcgt ccacagctac ctgaacctgc aaaacgccct
    12301 ggcccagcac gaggtgctgg agcagaccgc caagcagcgc aaggaactgg tcgacctgac
    12361 gcacggacgc cagaagtcag gcctggacac gatggtcgag gtcaggcagg ccgaatcggc
    12421 gctggcctcg gtgcaagtgg acaccaccca ggaagaaacc accatcgccc agttgcgcaa
    12481 ccagatcgcc gccctgctgg gcgccggtcc gcagcgcggc caggcgctag cccgcgtcca
    12541 gctcaccgtt ccgtccggcg tgctgcccgc cgccgtgccg ctggaactgc tgggccgccg
    12601 ccccgacgtc gccgccgcgc gctggcgcgc cgaggccgcc cgcagccaga tcggcgccgc
    12661 caaggcggat ttctatccca atgtgaacct gacggccttt gccggcttcc aggccctggg
    12721 cccgctcagc acgctcttcg acggcgggaa aaccgtcggc atcggcccgg ccatcacgct
    12781 gccgatcttc cacggcggcg aactgaacgc gcaattggcc ggccgccgcg ccgaggccga
    12841 tgtggccgtg gccgactaca acaacacggt gctcgacgcc gtgcgccaga cggccgacgc
    12901 catcgacgcc ctgcgcctga tcgagcgcga aaagcaggaa cagcaccagg cccgcaccgc
    12961 catcgaagac gcctacaagc tggcggtgga tcgctacaag gccggcctgg gcaactacct
    13021 gtccgtgctc atcgcccaga acagcgtgct ggcccaggcc cggctcgaca ccgacctgcg
    13081 catccgcgcc taccagctcg acgccgacct cgcctatgcg atgggcggcg gctaccagga
    13141 cccggatcag gccgccgccc aggccgcccg gaccgaagac aagacttccg actgaacacc
    13201 accaagctac tgacgtcatc atgactgaaa ccgcaactcc cctgcccaat cccaaccgca
    13261 cccgcctgct ggtcaccgca gcggtcatct tcgtcctgct cggcgtcgcc tacgccgtat
    13321 ggtggttctt gtacggctcg catttcgaag aaaccgacga tgcctacgtg cacggcaacc
    13381 tggtccagat cacgccgcag atcgcgggca ccgtggtctc gatcgacacc gacgacaccc
    13441 gttcggtgaa ggccggccag gccctggtcc gcatcgactc ctccgacgcc gacgtggcgc
    13501 tggcccaggc gcaggcccag ctggcgcaga ccgtgcgcca ggtgcgcacg cgctacgtgc
    13561 agaacgacgc cctggccgcc gacgtcgaag tgagccaggc cgagatcgtc cgcgcgcaaa
    13621 gcgacctcgc ccgcgcgcag agcgacctca agcgccgcca ggacctggcc cgttcgggcg
    13681 gcgtcagcgg tgaagaactg ctgcacgccg acagcgccgt gaagacggcg cagtccagcc
    13741 tggcccaggc caaggcgtcc ctggccgcct cgcaggccca tctggaaacc aatctggccc
    13801 tgacccacgg caccaccgtc gagcagcatc ccgacgtgct caatgccgcg gccgccgtgc
    13861 gctccgcatg gctggccaag tcgcgcaccg tgctgcccgc gccggtcgac ggcgtggtgg
    13921 cgcgccgcag cgtgcaggtg ggccagcgcg tggcccccgg cgccgtgctg atgaacgtgg
    13981 tgccgctcga ccaggtgtgg atcgaagcca acttcaagga aggccagctg agcaagatgc
    14041 agctcggcca gcccgccacg atcaagtccg acctgtacgg cagctcggtc acctaccacg
    14101 gcaccgtgat cggcatggac gccggcaccg gcagcgcctt tgccctgctg ccggcgcaga
    14161 acgccaccgg caactggatc aaggtcgtgc agcgcctgcc tgtgcgcatc gccatcgacc
    14221 ccgccgacct gaagcagcac ccgctgcgcg tcggcctgtc gaccgacgtc gaggtcgaca
    14281 ccagcgccgc cggcgacgcg gcccatgccg agcagagcaa ggccgcgctg ctgagcaccc
    14341 cggttttcga tccgtcccac ggcgaagccg acgcgatgat cgccggcatc atcaaggcca
    14401 acctgcaata aagaagccat gagcgacatc caagacggca ctcccgccca accggcggcg
    14461 ccggccggcc agccaacgcc acgcacctat ccgccgctca agggtggcaa gctcgtcctg
    14521 ggcgccgtgg cgctgtccac ggccgtgttc atgaacgtgc tcgacacgtc gatcgccaac
    14581 gtgtccatcc cgacgatctc gggcaacctg ggcgtgagct ccagccaggg cacctgggtg
    14641 atcacctcgt tcgccgtggc caacgccatc accgtgccgc tcacgggctg gctcacccag
    14701 cgcttcggcc aggtgcggct gttcctgatc tccaccctgc tcttcgtgct ggcctcgtgg
    14761 ctgtgcggct tctcgccctc gctcgaggcg ctgatcgcct tccgcgtgct gcagggcgcg
    14821 gtggctggcc ccatgatccc cctgtcgcag acgctgatgc tggccagctt ccccaaggag
    14881 aagtccggca tggcgctggc cttctgggcc atgaccaccc tggtcgcgcc ggtggccggg
    14941 ccgctgctgg gcgggtggat ctccgacaac tactcctggc cctggatctt ctacatcaac
    15001 gtcccggtgg gcctgctggc ggcctgggtg agctggcgca tctacaggga ccgcgaatcg
    15061 gtcacgcaca agctgcccat cgacaagatc ggcctggggc tgctggtggt gtgggtgggc
    15121 tgcctgcaga tcatgctcga caagggcaag gaactggact ggttcgaaag ctcgaccatc
    15181 gtcatcctgg ccgccacggt catcatcgcc ttcgtgttct tcctcatctg ggaactcacc
    15241 gacgcccatc cggtggtcga cctgcggcta ttcaaggtgc gcaatttcac cgtgggcgcc
    15301 atcaccctcg cggtggccta cggcatattc ttcggcaacg tggtgctgct gccgctctgg
    15361 ctgcaaagcg atatgggcta caccgccacc tatgccggca tggtgaccgc gcccgtgggc
    15421 atcctggcca tcatcctcac gcccatcgtc ggcaagctgc tggcccgctt cgacccgcgc
    15481 ctgatcgtca ccgtgtcctt cctgatcttc gccgccgtgt cgtggatgcg cgcgggcttc
    15541 aacaccgaca ccgacgtggg cacgctgatg atccccaccg tcatccaggg cgcggccatg
    15601 gcgggctttt tcgtgccgct gaccgccatc acgctgtcgc agatcgagcc ctggcgcatt
    15661 ccggcggcct cgggcctgtc gaacttcctg cggctgacgg ccggcggctt cggcacgtcg
    15721 atcgccacca ccctctggga aaaccgggcc tcgatgcatc acgcccagct caccgaaatc
    15781 gccaagccgg gacagcaggc cttcgaccag accatgagca cgctgcagag cggcggctac
    15841 agccagacgc agtcgctgtc gatgatcgat cgcatcgtcg acctgcaggc cttcaccatg
    15901 tcggtgaccg acatcttctt cgcttcggcg atggtcttcc tgctgctgac cggactggtg
    15961 tggttctcca ggacgcgcaa ggccggcaaa ggcggcggcg gcgcggccga ggcggcagcg
    16021 ggcgcgcact gagatcggat cgatccctgg tttacgggat aacccctgtc ccggcttgcg
    16081 gagaacacga agaaattcgg atacgctctt cgcctgaaca caccaaggaa gccgtctcat
    16141 gtcccgcccc gccgccattc ccaccgtaca gatcgacaac gaacacgtca ccgtgacgga
    16201 atggcgcttt ccaccgggcg gcgagaccgg ttggcaccgc cacggcatgc actacgtggt
    16261 ggtgccgcag accaccggcg aactgctgct cgaaacgccc cagggcgaga tgcgcagcca
    16321 gctcaccacc ggggtgtcct atacccgccc ggtcggggtg gagcacaatg tcgtcaaccc
    16381 caacgacacc gaattcgtct tcgtcgaaat cgaactcaaa aaagcctgaa cctgcgcagg
    16441 ggaacccatc atgagcagca ttgccgacat caagatcaac ggccagcgcc tgtggcagtc
    16501 gctgatggac ctggccagga tcggcgccac cgagaaaggc ggcaactgcc gcctggccct
    16561 gaccgacctc gaccgccagg gacgcgacct ggtggtgggc tggatgcgcg aggccggact
    16621 ggagatcacc gtcgaccaga tcggcaacat cttcggccgc cggccgggca agaaccccgc
    16681 cctgccctcg atcgccaccg gcagccacat cgacacccaa cccaccggcg gacgcttcga
    16741 cggctgcttc ggcgtgctcg ccggcctgga ggtgatgcgc accctgcagg aacagggcat
    16801 cgcgaccgag gcgccgctgg agctggccat ctggaccaat gaagaaggca gccgcttcgt
    16861 gcccgtgatg atgggctcgg gcgtgttctg ccatattttc tcgctggaaa ccgcgctgtc
    16921 ggccaccgac atcgacggca aaagcgtacg cgacgaactc gagaagatcg gctatgccgg
    16981 cgacgcgccg gtgggcgcgc gccagttcgg caagtacttc gaggcccata tcgagcaggg
    17041 tcccgtcctg gaggccgagg acaaggtcat cggcgtggtc accggatcgc tgggcgtctt
    17101 gtggtacgac tgcaccgtca cgggcatgga ggcccacgcc ggccccacgc ccatgtcgct
    17161 gcgccgcgac gcgctctacg cctcgacctt catcatgcag aaggtggtgg agatcgcgct
    17221 ggaatacgcg ccccagggcc gcggcaccgt gggcgccgcc tatgtgcacc cctgctcgcg
    17281 caacgtgatc cccggctcgg tgaccttcac catcgacatc cgccacgaaa ccgccgccaa
    17341 gctggccgag atggacgcca agctgcgcgc cgccatccag gaagtggccg acggcaagac
    17401 caccggccac gccatgcagg tcgagctcaa gccaagctcg cactttccgc ccacgccgtt
    17461 tgccgccgat atggtggcct cggtgcgcga gcaggcccgc gcccgcggct atgccgagat
    17521 ggacatcgtc accggcgccg gccacgacgc cgtgcacgtg gccaccgtct cgcccacggc
    17581 catgatcttc gtgccctgca aggacggcat cagccacaac gaggtcgagg atgccgatcc
    17641 cgcccacctg gaagccggcg ccaatgtgct gctgggcgcc atgctgacgc acgcggggct
    17701 cgccgggcgc tgatcacggc gcgcgccggt tcaaccagcg caaggcccgg catcccaggc
    17761 cgggccaata ctgcttcaat acccgcgctc gcgcgccgct aggcccgtgg tggcttcgcc
    17821 gcgcatgaag gcgcggcgct ggccgtcggc ctgctcgacg accgtctcgc gctgcaggct
    17881 aaatgatctt caatcgggaa tgcccctggc ctggatttgg cggataatgg ccgatgatat
    17941 accggtgccc ggacagcccg gcgccggtcc gtgccacaga ctacggaagc ggtattttca
    18001 tgaccacatt cagcaaagcc cagcagcagg acgtccagta ccacctgcac ccctacacca
    18061 acggcgtcaa gcaccgcgag tccggcccgc gcatcatcac gcgcggcgaa ggcatctacg
    18121 tctacgacga ccagggcaac cgctacctgg aaagcatggc cggcctgtgg agcgtcgccg
    18181 tcggcttcgg cgaaccccgc ctggtcgagg ccgccacccg gcaactgcag gccctgccct
    18241 actaccacac cttctcgcac aagtcgcacc ccagcgctat cgaactggcc gagcgcctgg
    18301 tgggctatac ccacggcaag atggcgcaag ccttcttcac caattcgggt tccgaggcca
    18361 acgataccgt cgtgaaaatg gtctggtact acaacaacgc cctgggccgc cgcgacaaga
    18421 aaaaaatcat cgcgcgcctg ggcagctacc acggcgtcac cgtggcatcg gccagcctga
    18481 ccggcctgcc cggcaaccac cgcgacttcg acctgccgct gccgcagatc aagcacacgg
    18541 ccaacccgca ctactaccgc tacggcaagg ccggcgaaac cgaggaagcc ttcgccaccc
    18601 gcctggcgca ggagctcgag gacctgatcc tgcgcgaagg ccccgattcg gtcgccgcct
    18661 tcatcggcga gcccgtcatg ggcgccggcg gcgtgatcat tccgcccaag acctattggg
    18721 aaaagatcca ggccgtctgc cgcaagtacg acctgctcat cgtcgccgac gaagtcatca
    18781 ccggcttcgg ccgcctgggc aagcgcttcg gcaccgaggt ctacgacatc gatgccgaca
    18841 tccaggtgct gtccaagcag atcacctcct cgtaccagcc cctgggcgcc gtgctcatca
    18901 acgccaaggt ggccgagacc atcaatcaga acagcggcaa gctgggcagc ttcggccacg
    18961 gctacaccgc cagcggccac cccgtggcca ccgccgtggc gctggaaaac ctcaagatca
    19021 tcgatgagcg cggcctcatc gacaacgccg ccgagatggg caagctcttg cgcagcgagc
    19081 tcgacgccaa actggccggc caccccctgg tgggcgagat ccgcagccag ggcctgatcg
    19141 ccgcggtcga gatggtggcc gaccgcgcca cccgcaagca gttcgacccg gtgggcaagg
    19201 ccggcggcta tgcctacgag cgcgcccaca gccacggcat catcgtgcgc ggcatcatgg
    19261 acaacgtcgc cgtctgcccg ccgctcatca tcaatgccga gcaggtgcgc gacatggtcg
    19321 accgcgtggc gcgcacggtg gacgataccg cggcgtattt gcgcggttaa ggcaatcgcg
    19381 atttacctta cgcgagcgaa gtttaggttc taaagacgtt cctgggcggt gggcataccc
    19441 cgcggcttcg ctgtgctggg ctcgggcgca accaggcgcc ctcggccccc tgcgggggct
    19501 accccatcac agaggcgccc cggggtatgc ccaccgccca ggaactcgca acaacctaat
    19561 gcgacggcct aaggccaacc gtgcgaccag gcatccgcag ctgcgcggac cgtgctcatg
    19621 acgctatgcg agtcctgcgt tcggcggaga ggccctggcg gcgcctctgt gacggggcag
    19681 cccgcgcagc gggccgaggg cgcctggttg cgcccga
//
LOCUS       DASWPO010000254         4945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3095448, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000254 DASWPO010000000
VERSION     DASWPO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3095448"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VGC69_13390"
     CDS             <1..390
                     /locus_tag="VGC69_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0296317.1"
                     /translation="EDPASRFVGGFLGKANVFAAEAAQHQGRACARIGEALVALEGQP
                     LPAGQVIVRPEKITFCEPDAAALPGRLKARVFQGNHWLCQVETSVGEVLVIRQNDGVP
                     VPPEGSVVHLRWRAPDMRPIDEARSAA"
     gene            404..1261
                     /locus_tag="VGC69_13395"
     CDS             404..1261
                     /locus_tag="VGC69_13395"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296318.1"
                     /translation="MNSLRSNPWVLSTPALLVYAVFLIVPLAMVFVISFFDFKFTGGM
                     QATFTWKNYIEIFSDGYYYEIYARTFGVSALVTLACAVLGTTEAYVLSRMRKPWKGLF
                     LMVVLGPLLTSVVVRTLGWALLFGSTGLISKALMALGLSDRPVSLMYTNLGTGIALVH
                     VLVPFMVISVWASLQRINPATEAAAMSLGASEFTVVRRVIVPQVMPGILSGSIMVFAM
                     AASAFATPAIVGGRRLKTVATAAYDEFLNTLNWPLGAALAVVLMIAMVVVLLAANRVV
                     ERRYGAVFN"
     gene            1270..2070
                     /locus_tag="VGC69_13400"
     CDS             1270..2070
                     /locus_tag="VGC69_13400"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296319.1"
                     /translation="MVTRNGPLSLIFHTLFILFILAPLVMVCAVAFTSLGYISLPEGG
                     LSLRHFRAIADNPQFIEAIWFSLGLGTLSATLAVMLALPGAMAIARNRFRGREALLAF
                     LMSPLMVPTVVLGLSFLKFFTDVGLAGTYAGLVIAHVVIIMPYALRLVLASATGIDPM
                     LERAALSLGATGWTAFRRVVLPLVLPGVISGWVIAFISSFDELTMSIFIASPSTTTLP
                     VRIFLHIQDTIDPLVTAVSALLIYVTAIAVFILDRVVGLENLFVGKGR"
     gene            2070..3236
                     /locus_tag="VGC69_13405"
     CDS             2070..3236
                     /locus_tag="VGC69_13405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0296320.1"
                     /translation="MNQQTSFPSLGGTVPRDGVYDAVVIGGGLVGSAVAYGLRRQLER
                     VAVLDEGDVAYRASRGNFGLIWVQSKGMGMARYSVWTQASAAAWPQLARELLEKTGVD
                     VRCEQKGGLQICLSDEELEARVNYMHALMAQPGMQPYPWKMLDRHELADMLPGIGPDV
                     RGASWSPLDGIANPLKLLRALHMAFAREQVDYLPRCPAQDIRFDDGIFSVQTPKGLLR
                     SRKLVVACGLGNKQMAAQVGMDVPVRPQRGQIIVLERTRRILDTPISTLRQMDEGSWL
                     IGDSQEEAGFADNVVGLPILGTLADRAVRTLPALRDVRAVRSWSALRVMSPDGFPIYE
                     QSATCPGAFVVTCHSGVTLAGAHALKMAPMIVNGMLDEDMSPFSTRRFHVQKAS"
     gene            3217..3522
                     /locus_tag="VGC69_13410"
     CDS             3217..3522
                     /locus_tag="VGC69_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0296321.1"
                     /translation="MFKRLAEAQGQASSAPVRITVDGAEVACRAGDSVAAALFASGRD
                     ACRDTVVREVPRGPYCMMGVCYDCLVTIDGQPNRQACMTLVCEGMAIERQHGAREVQ"
     gene            3519..>4945
                     /locus_tag="VGC69_13415"
     CDS             3519..>4945
                     /locus_tag="VGC69_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0296322.1"
                     /translation="MMQTCELLVIGAGPAGLAAAATAAALGVDTLLLDEQPAPGGQIY
                     RAITTTPVTDRRILGEDYWHGASLLEPFKISGARHEAGATVWSISRMTAPEPAEGFEV
                     GYSVGGQARLLHARHILLATGAQERPFPIPGWTLPGVITAGAAQILLKSAGLVPSGRA
                     VLAGCGPLLYLVAWQYLNAGAKIDLILETTPPGRLKQALPHAFDFLRSPYFGKGLKLM
                     RAVKAAIPIVKGVQGLKALGGDKLESVQYTVNGRTETVAADQLMLHQGVVPNVNLSRA
                     VGVEHEWNPALDCWQPRVDNWGASSVPGLGIAGDGAGIAGARAAEYRGLLAALNAACV
                     LGHIDAAQRDRDAMPYYRALVQATRGRAFFDTLFKAPDEYRRPTGDTIVCRCEEVTAG
                     QVRETVALGCSGPNQMKAFLRCGMGPCQGRFCGLTVSELIAEERGVPVPEIGYYRLRF
                     PTKPLSLGELASLPQTDESRQAVVR"
BASE COUNT          685 a         1700 c         1716 g          844 t
ORIGIN      
        1 gaagacccgg ccagccgctt cgtgggcggc ttcctgggca aggccaatgt ctttgccgcc
       61 gaggccgcgc agcaccaggg ccgggcctgc gcgcgcatcg gcgaggccct cgtggcgctc
      121 gaaggccagc cgctgccggc cggccaggtg atcgtgcggc ccgagaagat caccttctgc
      181 gaaccggacg ccgccgccct gccggggcgg ctcaaggccc gcgtgttcca gggcaatcac
      241 tggctgtgcc aggtcgagac ctcggtgggc gaggtgctgg tgatccgcca gaacgacggc
      301 gtgcccgtgc cgccggaggg cagcgtggtg catctgcgct ggcgcgctcc ggacatgcgg
      361 ccgatcgacg aggcgcgcag cgccgcctag cgccggaaac cccatgaatt ccctacgctc
      421 caacccctgg gtgctgagta cgccggcgct gctggtgtac gcggttttcc tgatcgtgcc
      481 gctggccatg gtgttcgtga tcagcttctt cgatttcaag ttcaccgggg gcatgcaggc
      541 cacctttacc tggaagaact acatcgagat cttctccgac ggctattact acgaaatcta
      601 cgcgcgcacc ttcggcgtct cggccctggt gacgctggcc tgcgccgtgc tgggcacgac
      661 cgaggcctat gtgctgtcgc gcatgcgcaa gccctggaag gggctgttcc tgatggtggt
      721 gctggggccg ctcctgacct cggtggtggt gcgcacgctg ggctgggcgc tgttgttcgg
      781 ctccaccggc ctgatcagca aggcgctgat ggccctgggc ctgtcggacc ggccggtgag
      841 cctgatgtac accaacctgg gcacgggcat cgcgctggtc catgtgctgg tgcccttcat
      901 ggtgatctcg gtgtgggctt ccctgcaacg catcaatccc gccaccgagg cggcggcgat
      961 gtcgctgggc gccagcgagt tcacggtggt gcggcgcgtg atcgtgccgc aggtcatgcc
     1021 gggcatcctg tctggttcca tcatggtgtt cgccatggcg gccagcgcct tcgccacgcc
     1081 ggccatcgtc ggcggccggc gcctgaagac ggtggccacg gcggcctacg acgaattcct
     1141 caacacgctg aactggccgc tgggcgccgc gctggccgtg gtgctgatga tcgccatggt
     1201 ggtcgtgctg ctggcggcca accgcgtggt cgagcgccgc tacggcgcgg tgttcaacta
     1261 ggggtcgcca tggttacccg caacgggccg ctcagcctga ttttccatac gctgttcatt
     1321 ctgttcatcc tggcgccgct ggtcatggtg tgcgcggtgg cattcacctc gctgggctat
     1381 atctcgctgc ccgagggcgg gctgtcgctg cgccatttcc gcgccatcgc cgacaatccg
     1441 cagttcatcg aggccatctg gttcagcctg gggctgggca cgctgtcggc cacgctggcc
     1501 gtcatgctgg cgctgcccgg cgccatggcg atcgcgcgca accgctttcg cggccgcgag
     1561 gcgctgctgg ccttcctgat gtcgccgctg atggtgccga cggtggtgct gggcctgtcg
     1621 tttctcaagt tcttcaccga cgtcgggctg gcgggcacct atgccgggct ggtgatcgcg
     1681 cacgtggtca tcatcatgcc ctatgccttg cgcctggtgc tggcctcggc caccggcatc
     1741 gatcccatgc tcgagcgcgc cgcgctgtcg ctgggcgcga cgggctggac ggctttcagg
     1801 cgcgtggtgc tgccgctggt gctgcccggc gtgatcagcg gctgggtgat cgccttcatc
     1861 tccagcttcg acgagctaac gatgtcgatc ttcatcgcct cgccctcgac caccacgctg
     1921 ccggtgcgca tcttcctgca tattcaagat acgatcgacc ccctggtgac ggcggtgtcg
     1981 gcgctcttga tctacgtgac ggccatcgcg gtcttcatcc tcgaccgcgt cgtcggactt
     2041 gaaaatttgt ttgtcggaaa aggacgttaa tgaatcaaca gacgagcttc ccgtctttgg
     2101 gaggcaccgt gccgcgcgac ggcgtctacg atgccgtggt catcggcggc gggctggtgg
     2161 gctcggcggt ggcctacggc ctgcgccgcc agctcgagcg ggtggccgtg ctcgacgagg
     2221 gcgatgtggc ctaccgcgcc tcgcgcggca atttcggcct gatctgggtg cagagcaagg
     2281 gcatgggcat ggcgcgctat tcggtctgga cccaggcctc ggccgcggcc tggccgcagc
     2341 tggcccggga gctgctggag aagaccggcg tggacgtgcg ctgcgagcag aagggcggcc
     2401 tgcagatctg cctctccgat gaagagctcg aggcgcgcgt gaactacatg cacgcgctga
     2461 tggcccagcc cggcatgcag ccctacccct ggaagatgct cgaccgccat gagctcgccg
     2521 acatgctgcc cggcatcggc cctgacgtgc gcggcgccag ctggtcgccg ctcgacggca
     2581 tcgccaatcc cttgaagctg ctgcgggcct tgcacatggc gttcgcgcgc gaacaggtcg
     2641 actacctgcc gcgctgcccg gcccaggaca tccgtttcga cgacggcatc ttttcggtcc
     2701 agacccccaa gggcctgctg cgctcgcgca agctggtggt ggcctgcggc ctgggcaaca
     2761 agcagatggc cgcgcaggtc ggcatggacg tgcccgtgcg cccgcagcgc ggccagatca
     2821 tcgtgctcga acgcacccgc cgcattctcg acacgccgat cagcacgctg cgccagatgg
     2881 acgaaggctc ctggctgatc ggcgactcgc aggaagaggc cggcttcgcc gacaacgtgg
     2941 tcggcctgcc catcctgggc acgctggccg accgggccgt gcgcacgctg ccggcgctgc
     3001 gcgacgtgcg cgcggtgcgc tcgtggtcgg cgctgcgggt gatgtcgccc gacggctttc
     3061 ccatctacga acaatccgcc acctgccccg gcgccttcgt cgtcacctgc catagcggcg
     3121 tgacgctggc cggggcgcac gcgctcaaga tggcgcccat gatcgtcaac ggcatgctcg
     3181 acgaagacat gtcgccgttt tccacccgga ggtttcatgt tcaaaaggct agctgaggcc
     3241 cagggccagg cttcgagcgc gccggtgcgc attacggtcg acggcgccga ggtagcctgc
     3301 cgcgccggcg acagcgtggc ggccgccctg ttcgccagcg gtcgcgacgc ctgccgcgac
     3361 accgtcgtgc gcgaggtgcc gcgcggcccc tactgcatga tgggcgtgtg ctacgactgc
     3421 ctggtcacca tcgacggcca gcccaaccgg caggcctgca tgacgctggt gtgcgagggc
     3481 atggccatcg agcgccagca cggcgcgcgc gaggtgcaat gatgcagaca tgcgaactgc
     3541 tggtgatcgg cgccgggccg gccggcctgg cggcggcggc cacggcggcc gcgctgggcg
     3601 tcgatacgct gctgctcgac gagcagcccg cgcccggcgg ccagatctac cgggccatca
     3661 ccaccacgcc ggttaccgac cgccgcatcc tcggcgaaga ctactggcac ggcgccagcc
     3721 tgctcgagcc cttcaagatc agcggcgcgc gccacgaggc gggcgccacg gtgtggtcga
     3781 tctcgcgcat gacggcgccc gaacccgcag agggcttcga ggtgggctat tcggtgggcg
     3841 ggcaggcgcg cctgctgcat gccaggcaca tcctgctggc cacgggcgcg caggagcgcc
     3901 cctttccgat tccgggctgg acgctgccgg gcgtgatcac ggccggcgcg gcgcagatcc
     3961 tgctgaagtc cgcgggcctg gtgcccagcg ggcgcgcggt gctggccggc tgcggcccgc
     4021 tcttgtacct ggtggcctgg caatacctga atgccggcgc caagatcgac ctcatcctcg
     4081 aaaccacgcc gcccggccgc ttgaagcagg ccctgccgca tgccttcgac ttcctgcgct
     4141 cgccctattt cggcaagggc ttgaagctga tgcgcgcggt caaggccgcc attcccatcg
     4201 tcaagggcgt ccagggcctg aaggcgctgg gcggcgacaa gctcgagtcg gtgcaataca
     4261 cggtaaacgg acgcaccgag accgtggccg ccgaccagtt gatgctgcac cagggcgtgg
     4321 tgcccaatgt gaacctgtcg cgcgcggtgg gcgtcgagca tgagtggaac ccggcgctgg
     4381 actgctggca gcccagggtc gacaactggg gcgccagctc ggtgccgggc ctgggcatcg
     4441 ccggcgacgg cgccggcatt gccggggcgc gggcggcgga atatcgcggc ctgctggcgg
     4501 cgctgaacgc ggcttgcgtg ctgggccaca tcgatgccgc ccagcgcgac cgcgacgcca
     4561 tgccgtacta ccgggcgctg gtgcaggcca cgcgcggccg cgccttcttc gacacgctgt
     4621 tcaaggcgcc ggacgaatac cgccgcccca cgggcgacac catcgtgtgc cgctgcgaag
     4681 aggtcacggc cggccaggtg cgcgagacgg tggccctggg ctgttccgga cccaaccaga
     4741 tgaaggcctt cctgcgctgc ggcatgggtc cttgccaggg ccgcttctgc ggcctgacgg
     4801 tgagcgagct catcgccgaa gaacgcggcg tgcccgtgcc cgaaatcggc tactaccgcc
     4861 tgcgcttccc caccaagccg ctctcgctcg gcgaactggc ctccctgccg cagaccgacg
     4921 aatcgcgcca ggccgtggtg cggat
//
LOCUS       DASWPO010000255         7917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1848543, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000255 DASWPO010000000
VERSION     DASWPO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848543"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VGC69_13420"
     CDS             complement(<1..631)
                     /locus_tag="VGC69_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0296323.1"
                     /translation="MSRELAVEQAGSFFDSGAFRQLLARRVAIPTESQNPARAPILAS
                     YLEDEMRPAFEAMGFECRVFTHPKARAPFLFAQRIEDPALPTVFGYGHGDVIRGLEAE
                     WKPGLAPWALTESQGRWYGRGIADNKGQHAVNIEALRIVIATRGKLGFNAKYLIEMGE
                     ETGSPGLRELCVEHKDLFAADLLIASDGPRLRAERPTVFLGARGSLNVDL"
     gene            complement(628..1587)
                     /locus_tag="VGC69_13425"
     CDS             complement(628..1587)
                     /locus_tag="VGC69_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY0296324.1"
                     /translation="MTPLLQAVSVSRTFTVRAGAFKPPRLLHAVNEVSLGVERGGVLG
                     IVGESGCGKSTLSRLLLGLIAPTRGNVELDGQDLTGINRRDLARRVQPVFQDPYSSLN
                     PRRSIASIVSLPLEVHGVDNPRQHKAIEMLERVGLPPRLANNTPGQLSGGQRQRVAIA
                     RALVMKPEIVVCDEPTSALDVSVQAQIINLLMELRREFNLTYVFISHNLAVVEHIATQ
                     VAVMYLGRVVESAPTAGLFARPQHPYTQALLASVLTPEPGLGIPDIGLGLSFPDPLNP
                     PTGCPFHPRCPHAMPQCAAERPALARLGAGLAACHLHLETHPS"
     gene            complement(1584..2621)
                     /locus_tag="VGC69_13430"
     CDS             complement(1584..2621)
                     /locus_tag="VGC69_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296325.1"
                     /translation="MIASSGNTAADIALDVQGLTVDIATPRGTLRAVRDVSFQVKRGE
                     TLCLVGESGCGKSMTSLAIMGLLPKAARRSATRLNLLGEDLARAGRRQMNALRGSRMA
                     MIFQEPMTALNPAYTIGDQLTEHYRLHKRAGAAQARDRAVELLGKVGIASAGERLGQY
                     PHQLSGGLRQRVMIAMALMCGPELLIADEPSTALDVTIQAQILRLLADLQAELGIAMV
                     LITHDLGVVARIAHRVAVMYAGQIVESSPVAQLFAAPRHPYTEGLIDCIPVPGRTRPG
                     EALGTIPGVVPALTGDLRGCAFVDRCGYAHPDCRNTVPVHEEHDRQWRCILTQTRARP
                     RAAPGLAGVQA"
     gene            complement(2618..3454)
                     /locus_tag="VGC69_13435"
     CDS             complement(2618..3454)
                     /locus_tag="VGC69_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296326.1"
                     /translation="MNRLLRNKALLIGGLLLILITLAALLAPWIAPHDPYAQDLVHRN
                     TPPIWYAKGGWDHILGTDQMGRDYLSRLIYGARISLLIGVSVALLSGLIGTTLGMAAG
                     YFGGRIDTVVSFLVTTRLSMPVILVALATVALVGNSLWVVILVLGLLIWDRFAVVMRS
                     ATQQVRSLEYVSAAQAAGASTWRIVRGEIMPNVVPQLIVIATLEAASAILLEASLSFL
                     GLGVQPPLPSWGLMISEAKAYMFFSFWLIAIPGCALAVLIFAINLAGDGLHQYLTPED
                     KA"
     gene            complement(3454..4371)
                     /locus_tag="VGC69_13440"
     CDS             complement(3454..4371)
                     /locus_tag="VGC69_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296327.1"
                     /translation="MFAFALKRLRLAVMVALTVSILAFMLMHLSGDPALAIAGDGATQ
                     ADIDMVRKTYGLDRPLVVQYADWVWHVLRGDFGTSVYFKVPAGPLILSKIGTTLKLGV
                     ASLCVALFIAIPLGVLAAIFKGGLVDRVCLGVAVLGQALPNFFFALLLIMFFSLSLHW
                     LPVSGSGTWQHYVMPAIALGYYAAPAFMRLIRAGMIEVLAADYIRTARAKGLSTRRVI
                     FKHALRNAVVPVVALTAVQLGYLLGGSVVIETIFALDGLGYLAYQSITFKDFPVIQLI
                     VLLLSVLYVLLTLASDLANAWLDPRIRVS"
     gene            complement(4373..5911)
                     /locus_tag="VGC69_13445"
     CDS             complement(4373..5911)
                     /locus_tag="VGC69_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296328.1"
                     /translation="MAVRIPVPFALRAGAAALLACLALASQPALAGKADDTLNFAYDQ
                     APESLDPYYNNVRIGVILAANVWDTLLYHDPVTGEYKGQLAKSFRQVDDKTLEIELRQ
                     GVKFHNGEDFDADSVVYTLTYVSDPKNKAVTQQNVNWIAKVEKLDQYKVRITTKEPFP
                     GAKEYLSTTAVMHPAKYYAEVGPAGMSAKPVGTGPYKVVDYQPGKVITLVKNDDYFKD
                     SPKGQPKIGKIVIRIIPDHQTQMAEAISGGEDLIMSVPKDQAEQLKSMPNLQVISGKT
                     MRIVFLQMNTQDGGPNPQFKDLRVRQAVLHAIDREAIFKNIVGDGEMINAICSPSQVG
                     CTQEGIPSYNYDPALAKKLLAEAGYPNGFDTEILAYRERDQTEAILHDLQAVGIHAKL
                     NFLQYAAMRDLSRSNKSAMSHQTWGSNLVNDTSASTPVYFASGADDVNKDPDLTALLN
                     QGDHTIDPAERTATYKKALSMIAEKAYAVPLWTLPAYYVANKDLNMTIYSDELPRFWN
                     MSWK"
     gene            6061..7113
                     /locus_tag="VGC69_13450"
     CDS             6061..7113
                     /locus_tag="VGC69_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0296329.1"
                     /translation="MNEASMREAIAYAQEHESEWDRSITGVFGVHQKDPAPWNRQWGP
                     LHDRGPASGVVVVDGKTLASWGEPERADLTYSVAKMYLALVTGIAWDQGLIRDLDQPI
                     GQQLPGIGFDSGNNALVTWRHLLQQTSEWEGERWGISDQADRYRAVTYGEPPAGNKGE
                     ARPLQKPGTYWEYNDVRINQLSYALMNLFRRPVPEVFREFVTRPLGCSEGWQWVGYDT
                     SWVEIDGRRMQSVPGGSHWCGGCSISSLDQALIGQMLLNAGKSRDGRQVLSEDWLRQM
                     REPCALAPYYGFLIWLNHQGKVFPSLPASSFFGVGAGSSYTWVEPERKMVVIARWLNS
                     AFSDGLFGRILAAVDA"
     gene            7190..>7917
                     /locus_tag="VGC69_13455"
     CDS             7190..>7917
                     /locus_tag="VGC69_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0296330.1"
                     /translation="MNARVDFSTAKPGPALRQALAGLPELAQRIGLDAAGRENRRELP
                     FFAFDLFRRSGLGALRIPEPWGGLGGSLEQLFEVLATLGAAEPNVAHALRLHFDVTET
                     LRLRPRDAHHDVQIGRVLAGAIYGGASTELGTERPGQISTTLVREGDHYRLTGRKYYS
                     TGTAFSDYGRINLKNEQGEDVVAMIPVQREGVQVVDDWDGMGQRMTASGSLVFDHVLV
                     HDDEVVPRPAGDLVTRHAGALRQL"
BASE COUNT         1270 a         2484 c         2903 g         1260 t
ORIGIN      
        1 tgaggtcgac gttgaggctg ccgcgcgcgc ccaggaacac ggtgggccgc tccgcgcgca
       61 ggcgcgggcc gtcggaggcg atgagcaggt cggcggcgaa gaggtcttta tgttccacgc
      121 acagctcgcg caggccgggc gagccggttt cctcgcccat ctcgatcagg tatttggcgt
      181 tgaagcccag cttgccccgc gtggcgatca cgatgcgcag ggcctcgatg ttgacggcgt
      241 gctggccctt gttgtcggcg atgccgcggc cgtaccagcg gccttgcgac tcggtcagcg
      301 cccagggggc caggcccggt ttccactcgg cctccaggcc gcggatcacg tcgccgtggc
      361 cgtagccgaa cacggtgggc agggccgggt cttcgatgcg ctgggcaaac aggaagggcg
      421 cgcgggcctt ggggtgggtg aacacgcggc actcgaagcc catggcctcg aaggccgggc
      481 gcatctcgtc ttccaggtag ctggccagga tgggcgcgcg cgcggggttc tggctctcgg
      541 tggggatggc gacgcggcgc gccagcaact ggcggaaggc gccggaatcg aagaaggatc
      601 cggcctgttc gacggcaagt tcgcggctca tgatgggtgg gtctccaggt gcaggtggca
      661 ggcggccagg cccgcgccga gccgggccag cgcggggcgc tcggcggcgc attgcggcat
      721 ggcgtggggg cagcgcggat ggaagggaca acccgtcggc ggattgagcg gatcgggaaa
      781 ggacaggccc aggccgatgt cggggatgcc cagcccgggc tcgggcgtga gcaccgaggc
      841 cagcagggcc tgggtgtagg gatgctgcgg ccgggcgaaa agcccggcgg tgggggcgga
      901 ctcgaccacg cggcccaggt acatcacggc cacctgggtg gcgatgtgct cgaccacggc
      961 caggttgtgg ctgatgaaga cataggtgag gttgaactcg cggcgcagtt ccatcagcag
     1021 gttgatgatc tgcgcctgca ccgagacgtc cagcgccgac gtgggttcgt cgcagacgac
     1081 gatctcgggc ttcatcacga gcgcccgcgc gatggccacg cgctggcgct ggccgcccga
     1141 gagctggccg ggggtgttgt tggccaggcg cggcggcagg cccacgcgct cgagcatttc
     1201 gatggccttg tgctggcgcg ggttgtccac gccgtgcacc tccaggggca gcgacacgat
     1261 ggaggcgatg gagcggcgcg ggttgagcga cgagtagggg tcctggaaca ccggctgcac
     1321 gcggcgcgcg agatcgcggc ggttgatccc ggtgaggtcc tggccgtcca gttcgacgtt
     1381 gccgcgggtg ggcgcgatga ggcccagcag caggcgcgac agagtggact tgccgcagcc
     1441 cgattcgccc acgatgccca gcacgccgcc gcgctcgacg cccaggctga cctcgttgac
     1501 ggcgtgcagc aggcggggcg gcttgaaggc gccggcgcgc acggtaaacg tgcggctgac
     1561 cgagacggcc tgcagcaggg gcgtcatgcc tggactccgg ccagaccggg ggcggcgcgg
     1621 gggcgggcgc gggtctgcgt gaggatgcag cgccattgcc ggtcgtgttc ctcgtgcacc
     1681 gggacggtgt tgcggcagtc ggggtgggcg tagccgcagc ggtcgacgaa ggcgcagccc
     1741 cggagatcgc cggtgagcgc cggcaccacg ccgggtatgg tgcccagcgc ctcgcccggg
     1801 cgcgtgcggc cgggcacggg gatgcagtcg atcagtcctt cggtataggg gtgccgcggc
     1861 gcggcgaaga gctgcgccac ggggctggac tcgacgatct ggccggcata catcaccgcc
     1921 acccggtggg cgatgcgcgc caccacgccc aggtcgtggg tgatgagcac catggcgatg
     1981 ccgagctcgg cctgcaggtc ggccagcagg cgcaggatct gcgcctggat ggtgacgtcc
     2041 agcgccgtgc tgggctcgtc ggcgatcagc agctcgggtc cgcacatcag ggccatggcg
     2101 atcatcacgc gctggcgcag gccgcccgag agctggtgcg ggtattgccc caggcgctcg
     2161 cccgccgagg cgatgcccac tttgcccagc agctccacgg cccggtcgcg cgcctgggcc
     2221 gcgccggccc gcttgtgcag gcgatagtgc tcggtgagct ggtcgccgat ggtgtaggcc
     2281 gggttgagcg ccgtcatggg ctcctggaag atcatggcca tgcggctgcc gcgcagcgcg
     2341 ttcatctgcc tgcggccggc ccgcgccagg tcttcgccca gcaggttcag gcgcgtggcg
     2401 ctgcgccgcg cggccttggg cagcaggccc atgatggcca gcgaggtcat ggacttgccg
     2461 cagcccgatt cgcccaccag gcacagggtt tcgccgcgtt tgacctggaa ggacacgtcg
     2521 cgcaccgcgc gcagggtgcc gcgcggcgtg gcgatgtcga cggtcaggcc ctgcacgtcc
     2581 agggcgatgt cggcggcggt gtttcccgaa cttgcgatca cgctttgtcc tcgggcgtca
     2641 ggtactggtg caggccgtcg ccggccaggt tgatggcgaa gatcagcacg gccagcgcgc
     2701 agccggggat ggcgatcagc cagaacgaga agaacatata ggccttggcc tcggagatca
     2761 tcagccccca ggacggcagg ggcggctgca cgcccaggcc caggaaggag agcgaggcct
     2821 cgagcaggat ggcgctggcg gcctcgagcg tggcgatgac gatgagctgc ggcaccacat
     2881 tgggcatgat ctcgccgcgc acgatgcgcc aggtggacgc gccggcggcc tgcgcggccg
     2941 agacgtattc cagcgagcgc acctgctggg tggcgctgcg catgaccacg gcgaagcggt
     3001 cccagatgag caggcccagc accaggatga cgacccacag cgaattgccc accagggcca
     3061 cggtggccag cgccaccagg atcaccggca tggagagccg ggtggtgacc aggaaggaca
     3121 ccacggtgtc gatcctgccg ccgaagtagc cggcggccat gcccagcgtg gtgccgatca
     3181 ggccggacag cagggccacg ctcacgccga tgagcagcga aatccgcgcg ccgtagatca
     3241 gccgcgacag gtagtcgcgg cccatctggt cggtgcccag gatgtggtcc cagccgccct
     3301 tggcatacca gatggggggc gtgttgcgat ggaccaggtc ctgcgcgtag gggtcgtgcg
     3361 gcgcgatcca gggcgcgagc agggcggcca gcgtgatgag gatcagcagc aagccgccga
     3421 tcagcagggc tttgttgcgc agcaggcggt tcatcacgac acccggatgc gcggatcgag
     3481 ccaggcgttg gcgagatcgg aggccagcgt cagcagcacg tacagcaccg acaggagcag
     3541 cacgatgagc tggatcacgg ggaagtcctt gaaggtgatg ctctggtagg ccaggtagcc
     3601 caggccgtcg agcgcgaaga tggtctcgat gaccaccgag ccgcccagca gatagcccag
     3661 ctgcacggcg gtgagcgcca ccaccggcac cacggcgttg cgcagcgcgt gcttgaagat
     3721 cacgcggcgg gtcgacaggc ccttggcgcg ggcggtgcgg atgtagtcgg cggccagcac
     3781 ctcgatcatg ccggcgcgga tcagccgcat gaaggccggc gcggcgtaat agcccagggc
     3841 gatggccggc atgacgtagt gctgccaggt gccgctgccc gagacgggca gccagtgcag
     3901 cgacagcgaa aaaaacatga tgagcagcag cgcgaaaaag aagttgggca gggcctgccc
     3961 cagcaccgcc acgcccaggc agacgcggtc gacgagaccg cctttgaaga tcgccgccag
     4021 cacgcccagc gggatggcga tgaacagcgc cacgcacagc gaggccacgc ccagcttgag
     4081 cgtggtgcct atcttggaaa ggattagcgg cccggccggc accttgaagt acaccgacgt
     4141 gccgaagtcg ccccgcagca catgccagac ccagtcggcg tattgcacga ccaggggccg
     4201 gtccagcccg taggtcttgc ggaccatgtc gatgtcggcc tgggtcgctc cgtcgcccgc
     4261 aatggccagg gcggggtcgc ccgacaggtg catcagcatg aaggccagga tggataccgt
     4321 cagcgccacc atcacggcca ggcgcaggcg tttcagcgcg aaggcgaaca tctcacttcc
     4381 agctcatgtt ccagaaacgg ggcagctcgt cggagtagat ggtcatgttg agatctttgt
     4441 tggcgacgta gtaggccggc agcgtccaga gcggcacggc ataggccttc tcggcgatca
     4501 tggacagcgc cttcttgtac gtggccgtgc gctcggcggg gtcgatggtg tggtcgccct
     4561 ggttgagcag ggccgtgagg tcgggatcct tgttgacgtc gtcggcgccc gaagcgaagt
     4621 acaccggggt ggaggccgag gtgtcgttga ccaggttcga gccccaggtc tggtgcgaca
     4681 tggcgctctt gttggagcgg gacaggtcgc gcatggcggc gtactgcagg aagttcagct
     4741 tggcgtggat gcccaccgcc tgcaggtcgt ggaggatggc ctcggtctgg tcgcgctcgc
     4801 ggtaggcgag gatctcggtg tcgaagccgt tcgggtagcc ggcctcggcc agcagtttct
     4861 tggccagggc cgggtcgtag ttgtacgagg ggatgccttc ctgcgtgcag ccgacctgcg
     4921 agggcgaaca gatggcgttg atcatctcgc cgtcgcccac gatgttcttg aagatggcct
     4981 cgcggtcgat ggcgtgcagc acggcctggc gcacgcgcag gtccttgaac tgcgggttcg
     5041 gcccgccgtc ctgcgtgttc atctgcagga acacgatgcg catggtcttg ccgctgatca
     5101 cctgcaggtt gggcatggac ttgagctgct cggcctggtc cttgggcacg ctcatgatca
     5161 ggtcttcgcc gcccgagatg gcctcggcca tctgcgtctg gtggtcgggg atgatgcgga
     5221 tgacgatctt gccgatcttg ggctggccct tgggcgagtc cttgaagtag tcgtcgttct
     5281 tcaccagggt gatgaccttg cccggctggt agtcgaccac tttgtacggg ccggtgccca
     5341 ccggcttggc gctcatgccg gccgggccga cctcggcgta gtacttggcc gggtgcatca
     5401 cggccgtggt ggacaggtat tccttggcgc cgggaaaggg ctccttggtg gtgatgcgca
     5461 ccttgtactg gtcgagcttc tcgaccttgg cgatccagtt gacgttctgc tgcgtgacgg
     5521 ccttgttctt cgggtcggac acataggtga gcgtgtacac caccgagtcg gcgtcgaagt
     5581 cttcgccgtt gtggaacttc actccctggc gcagctcgat ttccagcgtc ttgtcgtcga
     5641 cctgcctgaa gctcttggcc aactggccct tgtattcgcc cgtgaccggg tcgtggtaga
     5701 gcagggtgtc ccacacattg gcggccagga tcacgccgat gcgtacattg ttgtagtagg
     5761 gatcgaggct ctcgggcgcc tggtcgtagg cgaagttgag cgtgtcgtcg gccttgcccg
     5821 ccagggcggg ctgagaggcc agggccaggc aggccagcag ggcggctgcg ccggcgcgca
     5881 gcgcgaacgg gacggggatt cgaacggcca tatcggactt ccttttgggt aatcgattag
     5941 ggcaaaatat ggggccacta tatggcctgg acctgcactg caccatcggg tggataccct
     6001 agcggccggg tcgcgcgaag gcgcgcacaa tgacaacgac aagcaataca agggaaatcc
     6061 atgaatgaag catcgatgcg cgaggccatc gcctatgcgc aggaacacga atccgagtgg
     6121 gaccgcagca tcaccggcgt cttcggcgtg caccagaagg accccgcgcc ctggaaccgc
     6181 cagtggggtc ccttgcatga ccgcggcccg gcgtctggcg tggtggtggt cgatggcaag
     6241 acgctggctt cctggggcga acccgagcgc gccgacctga cctacagcgt ggccaagatg
     6301 tacctggccc tggtcaccgg catcgcctgg gaccaagggt tgatccgcga cctggaccag
     6361 cccatcggcc agcaattgcc gggcatcggt ttcgattcgg gcaacaacgc gctggtcacc
     6421 tggcgccact tgctgcagca gaccagcgaa tgggagggcg agcgctgggg catttccgac
     6481 caggccgacc gctaccgcgc ggtgacctac ggcgagccgc ccgccggcaa caagggcgag
     6541 gcgcgcccgc tgcaaaagcc cggtacctac tgggaataca acgacgtgcg catcaaccag
     6601 ctgtcgtacg cgctgatgaa cctgttccgc cggccggtgc ccgaggtgtt ccgcgagttc
     6661 gtcacgcggc cgctgggttg tagcgaaggc tggcaatggg tgggctacga cacctcctgg
     6721 gtcgagatcg acggccgccg catgcagtcg gtgccgggcg gctcgcactg gtgcggcggc
     6781 tgctcgatca gcagcctgga ccaggccctg atcggccaga tgctgctcaa cgcgggcaag
     6841 tcgcgcgacg gccgccaggt gctctcggaa gactggctgc gccagatgcg cgagccttgc
     6901 gcgctggcgc cgtattacgg cttcttgatc tggctgaacc accagggcaa ggtctttccc
     6961 agcctgccgg cctcgagctt cttcggcgtg ggcgcgggca gctcctacac ctgggtcgag
     7021 cccgagcgca agatggtggt catcgcgcgc tggctgaact cggccttctc cgacggcctg
     7081 ttcggcagga tattggcggc ggtggatgca taagcatccg ctttatttgt cgtagggccg
     7141 cgcgcggccg tgctccagaa ttcccggttt gatcaccggt gaacagagca tgaacgcgag
     7201 agtggatttc agtaccgcga agcccggccc cgcattgcgg caggccctgg ccggcttgcc
     7261 cgaactggcg caacggatcg gcttagacgc ggccggccgg gaaaaccggc gcgaactgcc
     7321 gttcttcgct ttcgacctgt tccgccgctc ggggctgggc gcgctgcgca tccccgaacc
     7381 atggggcggc ctgggcggat cgctggagca gttgttcgag gtcctcgcca ccctgggcgc
     7441 cgccgagccg aacgtggccc atgcgctgcg cctgcatttc gacgtgaccg agacgctgcg
     7501 cctgcggccg cgcgatgccc atcacgacgt gcagatcggc cgcgtcctcg ccggcgcgat
     7561 ttacggcggc gcctcgaccg agctcggcac cgaacgcccg ggccagatca gcacgacgct
     7621 ggtgcgcgaa ggcgaccatt accggctgac gggccgcaag tactactcca ccggcacggc
     7681 cttttccgac tacgggcgca tcaacctgaa aaacgagcag ggcgaggacg tcgtggccat
     7741 gatccccgtc cagcgcgaag gcgtgcaggt ggtcgacgat tgggacggca tgggccagcg
     7801 catgacggcc agcggcagcc tggtgttcga ccatgtgctg gtgcacgacg acgaggtggt
     7861 gccgcgcccg gcgggcgacc tggtaacccg ccatgcgggc gcattgcggc agttgca
//
LOCUS       DASWPO010000256        16308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2919086, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000256 DASWPO010000000
VERSION     DASWPO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 16308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16308
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2919086"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VGC69_13460"
     CDS             <1..472
                     /locus_tag="VGC69_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0296331.1"
                     /translation="VTPTLVQLVWIYYALPILTGVQLSSFTAVLLALGLHEGAYIGEI
                     FRAGVRSVAKGQFLAGQAIGMTYMQTMWHIILPQALKRMIPPLVNEFAALMKLTSLAS
                     VLAVPELLHAAYDIIATTFRPLEMYTVAAAVYAMVVAPFIILAHYLEHAWKERD"
     gene            568..1302
                     /locus_tag="VGC69_13465"
     CDS             568..1302
                     /locus_tag="VGC69_13465"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296332.1"
                     /translation="MSDISYDPFPKYLQIREIMLRWLASLQPGDKLPTEEQLGKRFSV
                     SRLTVRQALHTLEEEGIIARRPGVGTWLSRRPDTPVDNRLTGPIEEFSALGLAFSARP
                     IRQGTIDAPADVAAALGLPPGKRIYEVHRLRIVDGAPLLWLEVYLPTDLGRKVAKAAV
                     DGLFVPVLRRLYDQDVREQYQTIEALSASAELAEMLQVEPGSSILSVNRLFVDATGRR
                     VVFFKTRFRADRYFYTVNLPLARPRG"
     gene            complement(1317..2081)
                     /locus_tag="VGC69_13470"
     CDS             complement(1317..2081)
                     /locus_tag="VGC69_13470"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEY0296333.1"
                     /translation="MTAQSALDLNCDMGESYGAWKMGNDEAVLGYVSSANIACGFHGG
                     DPATLRQTVAAALKQGVAIGAHPSLQDLAGFGRRTMQISPQEAYDITVYQIGALAGVA
                     RSQGGALHHMKAHGALYNMAVKDAKLARALAQAVRDIDGDMIFYGLAGSVMIQEAEAL
                     GLRVAQEVFADRSYQPDGSLTPRTQPDAMITDVNKAVEQVLRMVQENAVIATDGTRVP
                     LKPDTLCIHGDQPNAVVFAQGIRKALQEAGIEVKTV"
     gene            complement(2154..3017)
                     /locus_tag="VGC69_13475"
     CDS             complement(2154..3017)
                     /locus_tag="VGC69_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEY0296334.1"
                     /translation="MKTNPLKTKLAAGQAVLAPGIYDPLSALIAQQAGFDALYLSGAS
                     IAYSHLGRSDVGLTTSTEVADTLARITDRVDCPIIVDGDTGFGNALNVQRTVRDFERA
                     GAAMIQLEDQGFPKRCGHLAGKTLVPAREMCGKLKAALDARTDANTLILARTDAVAVE
                     GLDAALERAEQYLACGVDALFVEALQTPEQIEAACTRFASRIPLLANMVEGGKTPVES
                     AQALARRGFRIVIFPGGTMRAVAHTLQGYYASLREHGTTAPWRDKMLDFDGLNAVIGT
                     PELLALGQRYE"
     gene            complement(3014..3547)
                     /locus_tag="VGC69_13480"
     CDS             complement(3014..3547)
                     /locus_tag="VGC69_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEY0296335.1"
                     /translation="MTNAPAIHRVWKIGSDIDTDQLAPGAYMKFGIDEIAKHCLQGIR
                     PDFAGGARPGDVLVAGENFGIGSSREQAAAALVALGLRAVLAPSYSGLYFRNAFNVGL
                     LLLTCPRCGELEENERIAIDLDTDAPIVARADGSALPCEPIPGFLLDMVRAGGLLNQL
                     KHRLATGELARHPMKAS"
     gene            complement(3544..4815)
                     /locus_tag="VGC69_13485"
     CDS             complement(3544..4815)
                     /locus_tag="VGC69_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0296336.1"
                     /translation="MTAQTLAQKLLAAAAGREAVRVGDILNCRVDLAMFHDSSGPRRL
                     KPMLEELGTGLWDRAKVVLVMDHYVPESDDESRRIVRIARDWAHEQALPHVYDSIGIC
                     HAVVPEHGHIRPGMFCVGGDSHSPTGGAFGAYMFGVGSTEMLGVVASGEIWIKVPQTL
                     LMHWRGRLADGVTAKDMALHMIGRFGMSGGRYQAAEFGGEAIRALSMQERMTLSNMSA
                     ELGSQAGLIAPDDTTLRYLREAGVPDDKLPDASAWQTDPDAQVERHDFDAAALAPQVA
                     APHSPANAAAVSDYPDTPIQAAYIGACTGAKFEDLLAADRVLRGKPVAAGVKLMVAPA
                     SLRDQQRAERAGIMARLVQSGAEVLPNSCGACSGYGGSIPEDANVISTTARNFKGRMG
                     SDTARVYLASPYTVAASALAGHVADPREFLA"
     gene            complement(4845..5624)
                     /locus_tag="VGC69_13490"
     CDS             complement(4845..5624)
                     /locus_tag="VGC69_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296337.1"
                     /translation="MSENPLPLYHQVYLLLKQRLAAGQYIYGAPMPGENALAAEYGVS
                     RLTVRRSLEALEAEGLIQRRQGRGTFAAPPAADGHSADIDALMTHLDQMGMQTQVRLL
                     DLQVVPASAQAAERLALAPGAPVSRSVRVRSHDGLPFSYLTTHVPHEIGQRIPRELLG
                     SRPLLAIFHDLGIEAAHAEQAISATLAEPAAAAALDIPVGSALLSLHRLVRDATGRPV
                     EWLHALYRPDRYEYRMHMQAYGTSGEPTWLPARPEPLPLRA"
     gene            complement(5656..6624)
                     /locus_tag="VGC69_13495"
     CDS             complement(5656..6624)
                     /locus_tag="VGC69_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296338.1"
                     /translation="MAFPALRLAACALLAALSITAAPAWAQNNAPIKLVVGAPPGGTT
                     DTVARGMAQFMAPVLNRTLVVENRPGAGGNIAADYVAKSKADGNTLLVTFTSFSINPS
                     LYKNLPFDTLKDFTPISMLATVPSVLVVRKDFPARNMSELIELVKAQPGKYTTAIGSI
                     GSSLHMAGERMKMMAKLDIVDIPYKGTNPALADLLGGHVDMMFASSVNVTPHIKSGAL
                     RALGVTSPQPLPEFPGVPPIGQSVPGFESNAWFALIGPAGLPPATLAALNDAARKAVQ
                     APAFRQMLENESAIPVGGSAEDLSAFIRQDIPRYAELVKFTGAKPE"
     gene            complement(6718..7737)
                     /locus_tag="VGC69_13500"
     CDS             complement(6718..7737)
                     /locus_tag="VGC69_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEY0296339.1"
                     /translation="MSITVLKPGLQSSFQDLGRIGFQHQGIPVNGAMDERAHRLASLL
                     VGNPATRATLEITLVGPVLRFDAPACFALAGADLGATLNGAPVPVLRPQLARAGDLLA
                     FPSRPEPERGLRAYLAVHGGYALEPVMGSESTYLRGGFGGLHGRALAKDDAVGLRQPL
                     RDDAASLDALDAAMAALRLYLPATLVNTPRRALRMLPGAHWDRFDAESQQRFVDTEFR
                     VSPASDRMGYRLAGPGIHQTSQRQMLSEAACFGSVQVPADGQPIVLMADRQTTGGYAK
                     IAQVISADLPLLAQYAPGRGLRFTLTTLDEAQRLDQARERAYAGLAAALAPLRERLAA
                     HPKAP"
     gene            complement(7734..8462)
                     /gene="pxpB"
                     /locus_tag="VGC69_13505"
     CDS             complement(7734..8462)
                     /gene="pxpB"
                     /locus_tag="VGC69_13505"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEY0296340.1"
                     /translation="MSAPNPFATHASPDWQIEAQGDRCLLVRFGDTLDARTGQACLAA
                     ARLLREAGLPGVTDIVPSFAAVAVHYRPGVDGPGHAQLAERVAALFAGGIPIDALAGR
                     DIEIPVCYGGELGPDLPEVAARAGISEDEVVSLHMRPGSMVFMLGFAPGFPYIGVHDE
                     KLAIPRRDTPRLSIPAGSVAIANRQSVIYPGGLPGGWSIIGATPLVMFDPARSPAALL
                     APGDRVRFTAIDRQAFERMGCGNT"
     gene            complement(8484..8918)
                     /locus_tag="VGC69_13510"
     CDS             complement(8484..8918)
                     /locus_tag="VGC69_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0296341.1"
                     /translation="MTTAHTAIQPFHLAFPVRDLEEARDFYGKKLGCPEGRSSPDWID
                     FNLFGHQIVAHLSPDACGAAATNAVDGHGVPVRHFGVVLTMGQWDAMASKLTDEGTEF
                     VIEPYIRFKGEVGEQATMFFFDPSGNALEFKAFKNMDSLFAK"
     gene            9051..9911
                     /locus_tag="VGC69_13515"
     CDS             9051..9911
                     /locus_tag="VGC69_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296342.1"
                     /translation="MLREFKTFIAVARGGTFTGAGRQLGLTQSAVSAQIRRLEQWLGA
                     ELFDRGAKSAELNAQGRELLPQAEALVGMAERMVDQAGAGRVSGLLRVGAIASAQQDL
                     LVRALARFRGDYPDVRVRIVPGVSLNLLGQVDAGEIDLAVMIRPSFALPPELAWQPLV
                     REPMVLAAPRHAPQASWRELLASQPFIRYDRSSFGGRLVDGFLRKQRIAVREALELDE
                     IDAIANLVRQGLGVALMPQTRQLATRGLRLVALDEPGFYREIGIVARRPLERAPLAAE
                     LVRCLVDAAG"
     gene            complement(9921..10847)
                     /locus_tag="VGC69_13520"
     CDS             complement(9921..10847)
                     /locus_tag="VGC69_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296343.1"
                     /translation="MLERIHLAVIREVDRQGSLTAAADVLYLTQSALSHTVKKMEQQL
                     GTAVWTREGRSLRLTQAGLYLLGLANRLLPQLEHAESLMQQYARGQRGTLRIGMECHP
                     CYQWLLKVVSPYLAKWPDVDVDVKQRFQFGGIGALFGYEIDVLVTPDPLHRPGLRYEP
                     VFDYEQVLVVGSSHRLAGQAHVTPQQLVDETLITYPVDIERLDIYGQFLLPAHCMPRR
                     HKVVEATDIMLQMVEAGRGVTALPGWLVEEYAGKMAIEPLRLGRRGIAKQIFLGVREA
                     DEDAEYLRAFVELARGVTWQVKGKRGARPRKP"
     gene            10962..13277
                     /gene="metE"
                     /locus_tag="VGC69_13525"
     CDS             10962..13277
                     /gene="metE"
                     /locus_tag="VGC69_13525"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY0296344.1"
                     /translation="MAKTHNLGFPRIGAQRELKFALENYWKGASTQDELAALGAQLRQ
                     RHWQDQAGLDLAPVGDFSFYDQVLDMSFTLGNIPPRALGLAGNRLDDYFRVARGRSAQ
                     DAACCAAHAGADAGAQGGVQAGEMTKWFDTNYHYIVPEFHAGTEFSLDASRLLEQIDQ
                     AHAQGVPFKPVIIGPVTYLWLGKAKDDSDKLALLPRLLPVYAELLDKLAERGAQWVQV
                     DEPALVTELPEAWRQAYAEAYRALQGSRAPLLLATYFGTLRHNLALACGLPVAGLHVD
                     AVRGRDEVEQVLRTLPSDKVLSLGVIDGRNIWKTDLHAVLAWLEPIHGELRDRLWIAP
                     SCSLLHVPVDLKAERRLDADIRSWLAFALQKLEELRVLAAALNQGRVAVQEALVANYS
                     AVAKRRESARVHNPAVQAALAALDAGAGRRTSPYARRAALQADRLKLPAYPTTTIGSF
                     PQTAEIRLARQRRQRGELDEAGYRAAMQAEIERCVREQEALDLDVLVHGEAERNDMVE
                     YFGEQLEGYAFSQFGWVQSYGSRCVKPPILFGDISRPRAMTVAWIQYAQSLTDKPMKG
                     MLTGPVTMLNWSFVRDDQPRAATCMQLALAIRGEVLDLEKAGVGVIQIDEAALREGLP
                     LRRSEWGEYLRWAVQAFRVTANGVRDETQIHTHMCYSEFNDIIASIADMDADVITIET
                     SRSDMELLDAFDDFAYPNEIGPGVYDIHSPNIPSREQMEGLMRKAAQRIPAQRLWINP
                     DCGLKTRGWSEVVPALRNMVAAARVLRAELR"
     gene            complement(13297..14637)
                     /gene="phoR"
                     /locus_tag="VGC69_13530"
     CDS             complement(13297..14637)
                     /gene="phoR"
                     /locus_tag="VGC69_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY0296345.1"
                     /translation="MVWLRTLIWCALWAAVGLLAGWVLAPDAGWAVLCLALFTMLMWR
                     SWRLTRVAQWAQDPEASPPASVGAWDGVLAPLYRYLRAQSRSLQESRNALQSMMAAAQ
                     ALPDGVVTLNEEFQIDWCNRMAMRHLGLRLPYDRGSSLLNLVRAPDFVAYAHGKDWPD
                     PILVRLSNSGQERVMMIQLATYGQNQRLLISRDVTQIEKLETTRRDFVANVSHELRTP
                     LTVLTGFLETLRDMPVDALPAEQREQYIGLMYEQAQRMQAIVEDLLTLSTLESSPGAG
                     PAVVNMSELLLHARQQVEALSNGQHIFEWHIDGDLDLLGSASELTSAVSNLLTNAVRY
                     TPDGGRISVYWEADAEGAAHYRVKDTGIGIAARHLPRLTERFYRVDRGRSRAVGGTGL
                     GLAITKHIVMRHDATLSIASEPGKGSTFALHFPPERVIRGEPQLPAPADTAPDA"
     gene            complement(14647..15345)
                     /gene="phoB"
                     /locus_tag="VGC69_13535"
     CDS             complement(14647..15345)
                     /gene="phoB"
                     /locus_tag="VGC69_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY0296346.1"
                     /translation="MSSTILVVEDEPAIQELIAVNLSFAGHKVLRAFDAEQAQTLIRA
                     ELPDLILLDWMLPGTSGLALARKLREDERTRTVPVIMLTAKGSEQDKVDGLEAGADDY
                     ITKPFSPKELMARIKAVLRRRAPQLTDDVIDVAGLKLDPVTHRLSGNTQSLSIGPTEF
                     RLLHFFMTHPERVFSRAQLLDQVWGDHVFVEERTVDVHIRRLRKALEPSGHDMHVETV
                     RGSGYRFTAQVPAR"
     gene            15499..16269
                     /gene="ubiE"
                     /locus_tag="VGC69_13540"
     CDS             15499..16269
                     /gene="ubiE"
                     /locus_tag="VGC69_13540"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY0296347.1"
                     /translation="MQNSNPPSGTSGQDSTHFGFQTVPENEKARKVAEVFHSVAANYD
                     IMNDLMSGGLHRIWKAFTISRAAIRPGMRALDIASGTGDLARAFAKRAGRTGEVWMTD
                     INDSMLRVGRDRMIDAGLVLPTAVCDAERLPFPDAYFDRVSVAFGLRNMTHKDRALAE
                     MTRVLKPGGKLLVLEFSRVAKPLAPVYDWYSFKVLPLMGKLVAKDEDSYRYLAESIRM
                     HPDQDTLARMMREAGLDRVQYFNLTAGVAALHEGVRLG"
BASE COUNT         2388 a         5238 c         5964 g         2718 t
ORIGIN      
        1 ggtcactccc acgctggtgc agctggtatg gatctactat gccctgccta tcctcaccgg
       61 cgtccagctg agttcgttca ccgcggtgct gctggcgctc ggcctgcacg aaggcgccta
      121 catcggcgag atttttcgtg ccggagtcag gtcggtggcc aagggccagt ttctggccgg
      181 ccaggccatc gggatgacct acatgcagac gatgtggcat atcatcttgc cgcaggcgct
      241 caagcgcatg attccgccgc tggtcaacga atttgccgcg ctcatgaaat tgacgtcgct
      301 cgcttcggtg ctggccgtcc ccgagctgct gcatgccgcc tatgacatca tcgcgacgac
      361 gttccggccg ctggaaatgt acacggtcgc cgccgccgta tatgccatgg tggtcgcgcc
      421 gttcatcatc ctggctcatt acctggagca tgcctggaaa gaacgcgatt gatccgcttc
      481 cagggtctcg cccggtcgcg gcgcttgttg ccttttatag gtaaagtggt agcggtaacg
      541 cactccggcc cctgaaaaat ccaaggcatg tccgatattt cctacgatcc ctttcccaag
      601 tacttgcaga tacgggaaat catgctgcgc tggcttgcat ccctgcagcc cggcgacaag
      661 ctgcccaccg aagagcagtt gggaaagcgt ttctccgtca gtcgcctgac tgtcaggcag
      721 gcgctgcata ccttggagga ggagggcatc atcgcgcggc ggcccggcgt ggggacctgg
      781 ttgtcccgcc gcccggatac ccctgtcgac aatcgcctga ccggtcctat cgaagagttc
      841 tcggccctgg gactcgcgtt cagtgcccgg ccgatccgcc agggcacgat agacgcgccg
      901 gcagacgtcg cggcagcgct ggggctgccg cccggcaagc gcatctatga ggttcaccgt
      961 ctgcgtatcg tggacggtgc gccgcttttg tggcttgaag tctatctacc caccgacctg
     1021 ggccgcaaag ttgccaaggc cgccgtggac ggtctgttcg tgcccgtgct gcgccgcctg
     1081 tacgaccagg acgtgcgcga gcaatatcaa accatcgagg cgctatcggc ttcggcggag
     1141 cttgcggaaa tgttgcaagt cgaaccgggc tcgtccatct tgagcgtcaa ccgcttgttc
     1201 gtcgatgcga ccggccgtcg tgtcgtcttc ttcaagacgc gctttcgggc cgaccgctat
     1261 ttctatacgg tcaatctgcc cctggctcgt cccaggggct gacacggcgg gtagcctcag
     1321 accgtcttca cctcgatccc ggcttcttgc agcgccttgc ggatcccttg cgcgaacacc
     1381 acggcattgg gctggtcgcc gtggatgcac agggtgtcgg gcttgagcgg cacgcgagtg
     1441 ccgtcggtgg cgatcaccgc gttttcctgc accatgcgca gcacttgttc cacggccttg
     1501 ttcacgtcgg tgatcatggc gtcgggctgg gtgcgcgggg tgagcgagcc gtcgggctgg
     1561 tagctgcggt cggcgaagac ttcctgggcg acgcgcaggc ccagggcttc ggcctcctgg
     1621 atcatgacgc tgccggccag gccgtagaag atcatgtcgc cgtcgatgtc gcgcacggcc
     1681 tgggccaggg cgcgggcgag cttggcgtcc ttgacggcca tgttgtagag ggcgccgtgg
     1741 gctttcatgt ggtgcagggc gccgccttgc gaacgggcca cgccggccag ggcgccgatc
     1801 tggtagacgg tgatgtcgta ggcttcctgc ggcgagatct gcatggtgcg gcggccgaag
     1861 ccggccagat cctgcaggct ggggtgggcg ccgatggcca cgccctgctt gagcgcggcg
     1921 gccacggtct ggcgcagggt ggcggggtcg ccgccatgga agccgcaggc gatattggcc
     1981 gaactgacgt agccgagcac ggcttcgtcg ttgcccattt tccaggcgcc gtagctttcg
     2041 cccatgtcgc agttcaggtc cagggctgat tgcgctgtca tagtttgttt tccgtcggtg
     2101 aagtctgtcg aaggtcaacg gtaatgtagc gccaaggcgg gccttcgtgc tgcttattcg
     2161 tagcgctggc ccagcgccag cagctcgggc gtgccgatca cggcgttgag cccgtcgaag
     2221 tccagcatct tgtcgcgcca cggcgcggtg gtgccgtgtt cgcgcaggct ggcgtagtag
     2281 ccctgcagcg tatgggcgac ggcgcgcatc gtgccgccgg ggaagatgac gatgcgaaag
     2341 ccgcgccggg ccagcgcctg ggcgctttcc acgggcgtct tgccgccctc gaccatattg
     2401 gccagcagcg ggatgcggga cgcgaagcgc gtgcaggccg cctcgatctg ctccggcgtt
     2461 tgcagggctt cgacgaagag cgcgtccacg ccgcatgcca ggtattgctc ggcgcgttcc
     2521 agcgcggcgt ccaggccttc caccgcgacg gcatcggtgc gcgccaggat cagggtattc
     2581 gcgtcggtgc gcgcatccag cgcggctttg agcttgccgc acatttcgcg ggccggcacc
     2641 agcgtcttgc cggccaggtg cccgcagcgc ttggggaagc cctggtcttc gagctggatc
     2701 atggccgcgc ccgcgcgttc gaagtcgcgc accgtgcgct gcacgttcag ggcgttgccg
     2761 aagcccgtgt cgccgtccac gatgatgggg cagtcgacgc gatcggtgat gcgcgccagg
     2821 gtgtcggcga cttcggtgct ggtggtcagc cccacgtccg agcgccccag gtgggaatag
     2881 gcgatcgagg cgcccgacag atacagcgcg tcgaagccgg cctgctgggc gatcagcgcc
     2941 gacaggggat cgtagatgcc gggggccagc acggcctggc cggcggcgag cttggttttg
     3001 agcggattgg ttttcatgat gccttcatgg ggtggcgcgc cagttcgccc gtggcgagcc
     3061 ggtgcttgag ttgattgagc aggccgcctg cgcggaccat gtccagcagg aaaccgggta
     3121 tgggttcgca gggcagggcg ctgccatcgg cgcgcgccac gatgggcgcg tccgtgtcca
     3181 ggtcgatggc gatgcgttcg ttttcttcga gttcgccgca gcgcgggcag gtgagcagaa
     3241 gcaggcccac gttgaaggca ttgcggaagt acaggccgct gtagctgggc gccagcaccg
     3301 cgcgcaggcc cagcgcgacc agcgcggccg cggcctgctc gcgcgacgag ccgatgccga
     3361 agttctcgcc ggccaccagc acgtcgccgg gccgtgcgcc gccggcgaag tccgggcgta
     3421 tgccttgcag gcagtgcttg gcgatctcgt cgatgccgaa tttcatgtag gcgccggggg
     3481 cgagctggtc ggtgtcgatg tcgctgccga ttttccagac ccggtggatg gcgggtgcgt
     3541 ttgtcatgcc aggaactccc gcgggtcggc gacgtggccg gccagggccg acgccgccac
     3601 ggtgtagggc gaggccaggt agacccgggc cgtgtccgag cccatgcgcc ccttgaagtt
     3661 gcgcgccgtg gtcgatatga cgttggcgtc ttcggggatc gagccgccgt agccggagca
     3721 ggcgccgcac gagttgggca gcacctcggc gccggactgc accagccgcg ccatgatgcc
     3781 tgcgcgttcg gcccgctgct ggtcgcgcag gctggccggc gccaccatca gcttgacgcc
     3841 tgcggccacg ggcttgccgc gcagtacgcg gtcggccgcg agcaggtctt cgaacttggc
     3901 gccggtgcag gcgccgatgt aggcggcctg gatgggcgtg tccgggtagt cggacacggc
     3961 cgccgcgttg gcggggctgt gcggggccgc cacctgcggc gccagcgcgg cggcgtcgaa
     4021 gtcgtggcgc tcgacctgcg cgtccgggtc ggtctgccag gccgaggcgt cgggcagttt
     4081 gtcgtccggc acacccgcct cgcgcaggta gcgcagcgtg gtgtcgtcgg gcgcgatcag
     4141 gccggcctgc gagccgagct cggccgacat gttcgacaag gtcatgcgct cctgcatgga
     4201 cagggcgcgt atcgcctcgc cgccgaactc cgcggcctgg tagcggccgc cgctcatgcc
     4261 gaagcggccg atcatgtgca gcgccatgtc cttggcggtg acgccgtcgg ccaggcggcc
     4321 gcgccagtgc atcagcagcg tctgcggcac cttgatccag atctcgccgc tggcgaccac
     4381 gcccagcatc tcggtgctgc ccacgccgaa catataggcg ccgaaggcgc cgccggtggg
     4441 cgagtgggag tcgccgccca cgcagaacat gccggggcgg atgtgcccgt gctcgggcac
     4501 cacggcgtgg cagatgccga tcgagtcgta gacgtgcggc agcgcctgct cgtgcgccca
     4561 gtcgcgggcg atgcgcacga tacggcgcga ttcgtcgtcg gactcgggca cgtagtggtc
     4621 catgaccagc acgaccttgg cgcggtccca caggcccgtg cccagctctt cgagcatggg
     4681 cttgagccgg cgcgggccgc tggagtcgtg gaacatggcc aggtcgacgc ggcagttcag
     4741 gatgtcgccg acgcgcacgg cctcgcggcc cgcggcggcg gcaagcagtt tctgggctag
     4801 ggtctgggcg gtcatgggga tgccttcaat gggttgcggt ggcctcaggc gcgcagcggc
     4861 aagggctcgg gcctggcagg cagccaggtg ggttcgccgg acgtgccgta ggcctgcatg
     4921 tgcatgcggt attcatagcg gtcggggcgg tagagggcgt gcagccattc cacgggccgg
     4981 ccggttgcgt cccgcaccag gcggtgcagg ctcagcagcg ccgatcccac ggggatgtcg
     5041 agcgcggccg ccgcggcggg ttcggccagg gtcgcggaga tggcctgctc ggcgtgggcc
     5101 gcttcgatgc ccaggtcgtg gaagatggcc agcagcggcc gcgatcccag cagttcgcgc
     5161 gggatgcgct ggccgatctc gtgcggcacg tgagtggtca ggtacgaaaa cggcaggccg
     5221 tcgtggctgc gcacgcgcac cgaccggctc accggcgcgc cgggggccag cgccaggcgt
     5281 tcggccgcct gcgcggacgc gggaaccacc tgcaggtcca gcagccgcac ctgggtctgc
     5341 atgcccatct ggtcgaggtg cgtcatgagc gcgtcgatgt cggcgctgtg cccgtcggcg
     5401 gcgggcggcg cggcgaacgt gccgcgcccc tggcgccgct ggatcaggcc ctcggcctcc
     5461 agcgcctcca gcgaacgccg gaccgtcagc cgcgaaaccc cgtactcggc cgccagcgcg
     5521 ttctcgccgg gcatgggggc gccgtatatg tattgcccgg cggcgaggcg ttgcttgagc
     5581 agcaggtaga cctggtggta caggggcagg gggttttcgg acatggcgag ggttctcgtg
     5641 cgcgccggcg ccaggctatt cgggcttggc gccggtgaat ttgaccagtt cggcgtagcg
     5701 gggaatgtcc tggcggatga aggccgacag gtcttcggcg ctgccgccga ccgggatggc
     5761 ggattcgttt tcgagcatct ggcgaaaggc cggggcctgc accgccttgc gcgcggcgtc
     5821 gttgagcgcg gccagggtcg ccggcggcag gccggccggg ccgatcagcg cgaaccaggc
     5881 gttcgattcg aagccaggga cgctctggcc gatgggcggc acgccgggga actcgggcag
     5941 cggctgcggg ctggtcacgc ccagggcgcg tagcgcgccg ctcttgatgt gcggcgtcac
     6001 gttcaccgaa ctggcgaaca tcatgtccac gtggccgccc agcaggtcgg ccagggcggg
     6061 gttggtgccc ttgtagggga tgtccacgat gtccagcttg gccatcatct tcatgcgctc
     6121 gccggccatg tgcagcgacg agccgatcga gccgatggcg gtcgtgtact tgcccggctg
     6181 cgccttcacc agctcgatca gttcggacat gttcctggcg gggaagtcct tgcgcaccac
     6241 cagcacgctg ggcacggtgg ccagcatgct gatcggagtg aagtccttca gcgtgtcgaa
     6301 gggcaggttc ttgtacaggc tggggttgat cgaaaagctg gtgaaggtca ccagcagggt
     6361 attgccgtcg gccttgctct tggcgacgta gtcggccgcg atgttgccgc ccgcgcccgg
     6421 gcggttttcc accaccaggg tccggttcag gacgggagcc atgaactggg ccatgccgcg
     6481 ggccaccgta tcggtcgtgc cgccgggagg cgcgccgacg accagcttga tgggggcgtt
     6541 gttctgggcc caggcgggcg ccgcggtgat ggacagggcg gccagcaggg cgcaggcggc
     6601 caggcgcaag gcgggaaatg ccatgttttt gtctccgtgt attaggttgt actgagaaca
     6661 atacaacaat acctataggc agacaatcct ccggacggcg cggcaggcgc gcgcttgtca
     6721 gggggctttg gggtgagcgg ccaaccgctc gcgcagcggc gcgagcgcgg cggccagccc
     6781 tgcgtaggcg cgttcgcgcg cctggtccag gcgctgggct tcgtccagcg tggtcagggt
     6841 aaagcgcagt ccccggccgg gcgcgtattg cgccagcagg ggcaggtcgg cgctgatgac
     6901 ctgggcgatc ttggcgtagc cgccggtggt ctggcggtcg gccatgagca cgatgggctg
     6961 gccgtcggcc ggcacctgga cggagccgaa gcaggcggcc tcggacagca tctggcgctg
     7021 gctggtctgg tggatgccgg ggccggcgag gcggtagccc atgcggtcgg aggccgggct
     7081 gacgcggaat tcggtgtcga cgaagcgttg ctgcgattcg gcgtcgaagc ggtcccagtg
     7141 cgcgcccggc agcatgcgca gggcgcggcg cggcgtgttc accagcgtgg cgggcaggta
     7201 caggcgcagg gcggccatgg ccgcgtcgag cgcatcgagg ctggccgcgt cgtcgcgcag
     7261 cggctggcgc aggccgacgg cgtcgtcctt ggccagggcg cggccgtgca ggccgccgaa
     7321 gccgccgcgc aggtaggtgc tctcgctgcc catgacgggt tcgagggcat agccgccatg
     7381 cacggccagg taggcgcgca ggccgcgttc cggctcgggg cgcgagggaa aggccagcag
     7441 gtcgccggcg cgggccagct gcgggcgcag cacgggcacg ggtgcgccgt tgagcgtggc
     7501 gcccaggtcg gcgccggcca gcgcgaagca ggcgggggcg tcgaagcgca gcaccgggcc
     7561 caccagggtg atctccagcg tggcgcgggt ggcgggattg ccgaccagca ggctggccag
     7621 gcggtgggcg cgttcgtcca tggcgccgtt cacggggatg ccctggtgct ggaagccgat
     7681 gcggcccagg tcctggaacg acgactgcag gccgggcttg aggacggtaa tgctcatgtg
     7741 ttcccgcaac ccatgcgctc gaaagcctgc ctgtcgatgg ccgtgaagcg cacgcggtcg
     7801 ccgggcgcga gcagcgcggc gggcgagcgg gccgggtcga acatcaccag cggcgtggcg
     7861 ccgatgatgc tccagccgcc gggcaggccg cccgggtaga tcaccgattg ccggttggcg
     7921 atggcgacgg acccggcggg aatgctcagg cgcggcgtgt cgcggcgggg gatggcgagc
     7981 ttttcgtcgt gcacgccgat gtacggaaaa cccggcgcga agcccagcat gaagaccatg
     8041 ctgccgggcc gcatgtgcag ggacacgacc tcgtcttcgc tgatgcccgc gcgggccgcc
     8101 acctcgggca ggtcggggcc gagctcgccg ccatagcaga cggggatctc gatgtcgcgg
     8161 ccggcgaggg cgtcgatcgg gatgccgccg gcgaagaggg ccgcgacgcg ttctgccagc
     8221 tgggcatgcc cgggaccgtc gacgccgggc cggtagtgca ccgccacggc ggcgaacgag
     8281 ggcacgatgt cggtgacgcc gggcaggccg gcctcgcgca gcaggcgcgc cgcggccagg
     8341 caggcctggc ccgtgcgggc atcgagcgtg tcgccaaagc gcaccagcag gcagcggtcg
     8401 ccctgggctt cgatctgcca gtcgggcgat gcgtgcgtgg cgaaagggtt cggggcgctc
     8461 aagcgggttg ctgcaggcgg tttttatttg gcgaagagcg aatccatgtt cttgaaggcc
     8521 ttgaactcga gggcgttgcc cgacggatcg aagaagaaca tggtggcttg ttcgccgact
     8581 tcgcccttga agcggatgta gggctcgatg acgaactcgg tgccttcatc ggtgagctta
     8641 ctggccatgg cgtcccattg gcccatggtc agcaccacgc cgaagtggcg caccggcacg
     8701 ccgtggccgt cgaccgcgtt ggtggcggcg gcgccgcacg cgtccgggga caggtgggcg
     8761 acgatctggt ggccgaaaag attgaagtcg atccagtcgg gcgaactgcg gccttcgggg
     8821 cagcccagct tcttgccgta gaagtcgcgg gcttcttcca ggtcgcggac cgggaaggcc
     8881 aggtggaagg gttggatggc ggtgtgggcg gtggtcatga cgaacgctcc aggcaaggtg
     8941 tcattaaagc aatttgttcc ggtccgctag tgtatgaatt gtttttttga ttggaaaacg
     9001 atatttttat ggacttagca tcgaaaaaaa tgattgattg agtcgccatc atgctgcggg
     9061 aattcaagac cttcatcgcc gtggcccggg gcggcacctt taccggggcc ggccgccagc
     9121 tggggctgac gcagtcggcg gtgagcgcgc agatacgccg tctcgagcaa tggctgggcg
     9181 ccgagctgtt cgaccgcggc gccaagtcgg ccgaactcaa tgcccagggc cgcgagctgc
     9241 tgccccaggc cgaggccctg gtgggcatgg ccgaacgcat ggtcgaccag gctggcgcgg
     9301 gccgggtgag cggcctgttg cgggtcggcg cgatcgcctc ggcgcagcag gacctgctgg
     9361 tgcgggcgct ggcgcgcttt cgcggcgact atcccgatgt gcgggtgcgc atcgtgccgg
     9421 gcgtgtcgct caacctgctg ggccaggtcg atgccggcga gatcgacctg gccgtgatga
     9481 tccgtccgtc gttcgcgctg ccgcccgagc tggcctggca gccgctggtg cgcgagccca
     9541 tggtgctggc ggcgccgcgc catgcgccgc aggcctcgtg gcgcgagctg ctggccagcc
     9601 agcccttcat ccgctacgac cgctcgtcct tcggcggcag gctggtggac gggtttctca
     9661 ggaagcagcg cattgccgtg cgcgaggcgc tggagctcga cgagatcgac gccatcgcca
     9721 acctggtgcg gcagggcctg ggcgtggcct tgatgccgca gacgcggcaa ctggccacgc
     9781 gcggcctgcg cctggtcgcg ctcgacgagc ccggcttcta ccgcgagatc ggcatcgtgg
     9841 cgcggcgccc gctggagcgc gcgcccctgg cggccgagct ggtgcgctgc ctggtcgacg
     9901 cggcgggctg atgtcccgac ctagggcttg cgcggcctgg ccccgcgttt gcctttgacc
     9961 tgccaggtga cgccgcgcgc cagctcgacg aaggcccgca ggtactcggc gtcttcgtcg
    10021 gcctcgcgca cgcccaggaa gatctgcttg gcgatgccgc ggcgtcccag gcgcagcggt
    10081 tcgatcgcca tcttgccggc gtactcctcg accagccagc cgggcagggc cgtgacgccg
    10141 cggccggcct ccaccatctg cagcatgatg tcggtggctt cgaccacctt gtggcgccgg
    10201 ggcatgcagt gggcgggcag caggaactgg ccatagatgt ccagccgctc gatgtccacc
    10261 gggtacgtga tgagggtttc gtcgaccagc tgctgcggcg tgacgtgggc ctggccggcc
    10321 aggcggtggc tgctgccgac caccagcacc tgctcgtaat cgaagaccgg ctcatagcgc
    10381 agcccgggcc gatgcagggg gtcgggcgtg acgagcacgt cgatctcgta gccgaacagg
    10441 gcgccgatgc cgccgaactg gaagcgctgc ttgacgtcca cgtccacgtc cggccacttg
    10501 gccaggtagg gggagacgac cttgagcagc cactggtagc agggatggca ttccatgccg
    10561 atgcgcagcg tgccgcgctg gccgcgggca tattgctgca tgagcgattc ggcgtgttcc
    10621 agctgcggca gcagccggtt ggccaggccc agcaggtaga gcccggcctg cgtcaggcgc
    10681 aggctgcggc cttcgcgcgt ccagacggcc gtgcccagct gctgctccat tttcttcacg
    10741 gtatggctga gcgccgactg ggtcaggtac agcacgtcgg ccgcggcggt cagcgaccct
    10801 tggcggtcga cttcgcggat gacggcgaga tggatgcgtt cgagcatggt tccaatatat
    10861 gaatgaattt gatgggtgaa tgaaaaaata ccactatttt tcatggttcg ggctgcctac
    10921 catggacgct cttttcgcat tcggtaccag gacagcacat catggccaag acccacaacc
    10981 tcggatttcc gcgcatcggc gcgcagcgcg aactcaagtt cgcgctcgaa aactattgga
    11041 aaggcgcgtc cacgcaggac gagctcgccg cgctgggcgc gcagctgcgc cagcgccatt
    11101 ggcaggacca ggccgggctg gacctggcgc cggtgggcga tttctcgttc tacgaccaag
    11161 tgctcgacat gagcttcacg ctgggcaata tcccgccgcg cgcgctcggc ctggcgggca
    11221 accgcctgga cgactatttc cgcgtggccc gcggccgttc cgcgcaggac gcggcctgct
    11281 gcgcggcgca tgccggcgcg gatgccggcg cgcagggtgg tgtccaggcc ggcgagatga
    11341 ccaagtggtt cgacaccaac taccactaca tcgtgccgga gttccatgcc ggcaccgagt
    11401 tctcgctcga cgcctcgcgc ctgctggagc agatcgacca ggcgcatgcc cagggcgtgc
    11461 cgttcaagcc ggtcatcatc gggcccgtca cctatctgtg gctgggcaag gccaaggacg
    11521 attccgacaa gctcgccttg ctgccgcgcc tgctgccggt ctatgccgag ctgctcgaca
    11581 agctggccga gcggggcgcc cagtgggtgc aggtggacga gccggcgctg gtcacggaat
    11641 tgcccgaagc ctggcggcag gcctatgccg aggcctaccg ggccctgcag ggcagccgcg
    11701 cgccgctgct gctggcgacc tactttggca ccctgcggca caacctggcg ctggcctgcg
    11761 gcctgcccgt ggcggggctg cacgtcgacg ccgtccgcgg ccgcgacgag gtggagcagg
    11821 tgctgcgcac cctgccgtcg gacaaggtcc tgtccctggg cgtgatcgac ggtcgcaata
    11881 tctggaagac cgatctgcac gcggtactgg cctggctgga gccgattcac ggcgaattgc
    11941 gcgaccgctt gtggatcgcg ccttcctgct ccctgctgca tgtgccggtc gacctgaaag
    12001 cggagcggcg gctcgatgcc gacatccggt cctggttggc cttcgcgctg cagaagctcg
    12061 aggagctgcg ggtgctggcc gcggccctga accaggggcg cgtggccgtg caggaggccc
    12121 tggtcgccaa ttactcggcg gtggccaagc gccgcgagtc ggcccgcgtg cacaatccgg
    12181 ccgtgcaggc agcgctggcc gcgctcgacg ccggggctgg gcggcggacg tcgccctatg
    12241 cgcgccgcgc cgcgctgcag gccgatcgcc tgaagctgcc cgcctatccc accacgacca
    12301 tcggctcctt tccccagacg gcggagatcc gcctggcccg ccagcgccgc cagcgcggcg
    12361 aactcgacga ggcgggctac cgcgccgcca tgcaggccga gatcgaacgc tgcgtgcgcg
    12421 agcaggaggc gctggacctg gacgtgctgg tgcatggcga ggccgagcgc aacgacatgg
    12481 tcgagtactt cggcgagcag ctggagggct atgcgttcag ccagttcggc tgggtgcagt
    12541 cctacggctc gcgctgcgtc aagccgccca tcctgttcgg cgacatcagc cggcccaggg
    12601 ccatgacggt ggcgtggatc cagtacgcgc agtcgctgac cgacaagccc atgaagggca
    12661 tgctgaccgg gccggtcacg atgctgaact ggtcgttcgt gcgcgacgac cagccgcgcg
    12721 cggcgacctg catgcagctg gcgctggcca tccgcgggga agtgctggac ctggaaaagg
    12781 ccggcgtggg cgtgatccag atcgacgagg cggcgctgcg cgaaggtttg cccctgcgcc
    12841 gttcggaatg gggcgagtac ctgcgctggg cggtgcaagc cttccgcgtc acggccaacg
    12901 gcgtgcgcga cgagacgcag atccacaccc atatgtgcta ttcggagttc aacgacatca
    12961 tcgcctcgat cgccgacatg gatgccgacg tcatcaccat agaaacctcg cgctccgaca
    13021 tggagctgct cgatgccttc gacgattttg cctatcccaa cgaaatcggg cccggggtgt
    13081 acgacatcca ttcccccaac atcccgtcgc gcgaacagat ggaaggcctc atgcgcaagg
    13141 ccgcgcagcg cattcccgcc caacgcctgt ggatcaatcc ggattgcggc ctgaagacgc
    13201 ggggctggag cgaagtggtc ccggcgctgc gcaatatggt ggcggcggcc cgcgtcctgc
    13261 gcgcggaatt gcgctgagcc ggttcgcgcc cgccgctcag gcgtcgggcg ccgtgtcggc
    13321 cggggcgggc agctggggct cgccgcggat gacgcgttcg ggcgggaagt gcagcgcgaa
    13381 cgtgctgccc ttgcccggct cgctggcgat gctgagcgtg gcgtcatggc gcatgacgat
    13441 gtgcttggtg atggccaggc ccaggccggt gccgcccacg gcgcgcgaac ggccgcggtc
    13501 cacgcggtag aagcgttcgg tcaggcgcgg aaggtggcgc gccgcgatgc cgatgccggt
    13561 gtccttcacg cggtagtggg ccgcgccttc ggcgtcggct tcccagtaca ccgagatgcg
    13621 tccgccgtcg ggcgtgtagc gcacggcgtt ggtcagcagg ttcgagacgg ccgaggtcag
    13681 ctcgctggcg ctgcccagca ggtccaggtc gccgtcgata tgccattcga agatatgctg
    13741 gccgttggag agggcctcga cctgctggcg cgcgtgcagc agcaattcgc tcatgttgac
    13801 gaccgcgggc ccggcgccgg gcgaggattc gagcgtggac agcgtgagca ggtcttcgac
    13861 gatggcctgc atgcgctgcg cctgctcgta catcaggccg atgtattgct cgcgctgctc
    13921 ggcgggcagg gcgtcgacgg gcatgtcgcg caaggtttcc aggaagcccg tcaacaccgt
    13981 gagcggggtg cgcagttcgt gcgagacgtt ggccacgaag tcgcgccggg tggtttccag
    14041 cttctcgatc tgcgtgacgt cgcgcgagat cagcaggcgc tggttctgcc cgtaggtggc
    14101 gagctggatc atcatcacgc gctcctgccc gctattggac aggcgcacca ggatgggatc
    14161 gggccaatcc ttgccgtgcg cgtaggccac gaagtcgggc gcgcgcacca ggttgagcag
    14221 gctgctgccg cggtcgtagg gcaggcgcag gcccaggtgg cgcatggcca tgcggttgca
    14281 ccagtcgatc tggaattctt cgttgagcgt gaccacgccg tcgggcaggg cctgggcggc
    14341 ggccatcatg ctctgcaggg cattgcggct ttcctgcagg gagcgcgatt gcgcgcgcag
    14401 gtagcggtag agcggcgcca gcacgccgtc ccaggcgccc acggaggcgg gcggggaggc
    14461 ttcggggtcc tgcgcccact gcgccacgcg cgtcaggcgc cagctgcgcc acatcagcat
    14521 ggtgaacagc gccaggcaaa gcaccgccca gccggcgtcg ggcgccagca cccagcccgc
    14581 gagcagccct acggccgccc agagggcgca ccagatcaat gtacgcagcc agaccatgcc
    14641 ggcagtctag cgtgccggta cttgcgccgt gaaccggtag ccgctgccgc gcacggtttc
    14701 cacgtgcatg tcatggccgc tgggttccag cgctttgcgc aggcggcgga tgtgcacgtc
    14761 gaccgtgcgc tcctcgacga agacgtggtc gccccacacc tggtcgagca gctgggcgcg
    14821 cgagaacacg cgctcgggat gggtcatgaa gaagtgcagg aggcggaatt cggtcggccc
    14881 gatcgacagc gactgcgtgt tgcccgacag gcggtgggtg accggatcga gcttgaggcc
    14941 ggccacgtcg atgacgtcgt cggtgagctg gggcgcgcgg cggcgcagca cggccttgat
    15001 gcgggccatc agctccttgg gcgagaaggg cttggtgatg tagtcgtcgg cgccggcttc
    15061 caggccgtcg accttgtcct gctccgagcc cttggcggtg agcatgatga cgggcacggt
    15121 gcgggtgcgc tcgtcttcgc gcagcttgcg cgccagggcc aggcccgagg tgccgggcag
    15181 catccagtcg agcaggatca ggtcgggcag ttcggcgcgg atcagcgtct gggcctgttc
    15241 ggcgtcgaag gcccgcagca ccttgtgccc ggcgaatgac aggttgacgg cgatcagttc
    15301 ctggatggcg ggttcgtctt cgacgacgag tatggtgctg gacatgggag cgggtcgctg
    15361 gcaaaaacgg tgcgccggtt ggcgcgagcg ttgcaatagt atggccgtaa tatgtcaggt
    15421 atgtgaccgg cgctgcggga taccgccggg gcgcgggggc atgatcggct accattgcgc
    15481 cattaccggg atcacaccat gcagaattcc aatcccccct cgggtacgtc cgggcaggac
    15541 agcacccatt tcgggtttca aaccgtcccc gaaaacgaaa aggcccgcaa ggtcgccgag
    15601 gtgttccatt cggtggccgc caactacgac atcatgaacg acctgatgtc gggcggcctg
    15661 caccgtatct ggaaggcctt caccatcagc cgggccgcga tccgccccgg catgcgcgcg
    15721 ctggacatcg ccagcggcac gggcgacctg gcccgcgcgt tcgccaagcg cgccgggcgc
    15781 accggcgaag tctggatgac cgacatcaac gactccatgc tgcgcgtggg gcgcgaccgg
    15841 atgatcgacg ccggcctggt gctgcccacc gccgtgtgcg atgccgagcg gctgcctttt
    15901 cccgacgcgt atttcgaccg cgtcagcgtg gccttcggcc tgcgcaacat gacgcacaag
    15961 gaccgcgcgc tggccgagat gacgcgcgtg ctcaagcccg gcggcaagct gctggtgctg
    16021 gagttctccc gcgtggccaa gccgctggcg ccggtctacg actggtactc cttcaaggtg
    16081 ctgcccctga tgggcaagct ggtggccaag gacgaggaca gctaccgcta cctggccgag
    16141 tcgatccgca tgcaccccga ccaggacacc ctggcccgca tgatgcgcga ggccggcctg
    16201 gaccgcgtgc agtacttcaa cctgaccgcc ggcgtggcgg cgctgcacga gggcgtgcgg
    16261 ctgggttgac gaccggcccg ccggcgccgg cggggatcgt cgcttgtg
//
LOCUS       DASWPO010000257         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2652127, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000257 DASWPO010000000
VERSION     DASWPO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652127"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /gene="rho"
                     /locus_tag="VGC69_13545"
     CDS             complement(<1..1157)
                     /gene="rho"
                     /locus_tag="VGC69_13545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY0296348.1"
                     /translation="MHLNELKAQHVSQLLEMAAGLEIENANRLRKQELMFAIMKRRAK
                     QGEQIFGDGVLEVLPDGFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDSIEGEVR
                     VPKDGERYFALVKVDKINSVLPEAAKHRIMFENLTPLHPDRVMRLEREIKSEENLTGR
                     IMDIFAPIGHGQRGLIVSPPKSGKTVMMQHIAHAITTNYPEAVMIVLLVDERPEEVTE
                     MQRTVRGEVVASTFDEPATRHVQVAEMVIEKAKRLVEMKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNVEEGGSLTIIGSALIETGSRMDDVIYEE
                     FKGTGNMEVHLERRLAEKRVYPSINLNKSGTRREELLIPKEILQKVWILRKL"
     gene            complement(1413..1739)
                     /gene="trxA"
                     /locus_tag="VGC69_13550"
     CDS             complement(1413..1739)
                     /gene="trxA"
                     /locus_tag="VGC69_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY0296349.1"
                     /translation="MSEQIKHVSDASFDTDVLQSGQPVLVDYWAAWCGPCKMIAPILE
                     EVAGEYAGRLTVAKVDVDANPDTAAKYGIRGIPTLMLFKDGKAAATKVGALSKSQLTA
                     FLNGTL"
     gene            1964..>2950
                     /locus_tag="VGC69_13555"
     CDS             1964..>2950
                     /locus_tag="VGC69_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0296350.1"
                     /translation="MATPRYTEASIRVLKGLEPVRQRPGMYTRTENPLHIVQEVIDNA
                     ADEALAGHGKQIQVTMHADGSVSVEDDGRGIPVGLHPEEHAPVVELVFTRLHAGGKFD
                     KAAGGAYAFSGGLHGVGVSVTNALATKLEVVVWRDGGTHRMVFAGGDVVEPVAPFDAG
                     VKKKSGTRVRVWPDPKYFDSPVIPLGELTHLLRSKAVLLPGVKVTLKNEKSGDTKTWQ
                     YTDGLRGYLAEALAGAELMIPFFEGEQYADADHENFAEGEGAQWAVAWTEDGNVVRES
                     YVNLIPTPAGGTHESGLREGLFGAVKSFADLHGLLPKGVKLLPEDVFARASFV"
BASE COUNT          465 a          905 c         1009 g          571 t
ORIGIN      
        1 agcttgcgca ggatccacac cttctggagg atttccttcg ggatcagcag ttcctcgcga
       61 cgggtgcccg acttgttcag gttgatcgac gggtacaccc gcttttcggc cagccggcgc
      121 tccaggtgca cttccatgtt gccggtgccc ttgaattcct cgtagatcac gtcgtccatg
      181 cggctgccgg tctcgatcag cgcgctgccg atgatggtca gcgagccgcc ttcctccacg
      241 ttgcgcgccg cgccgaagaa gcgcttgggg cgctgcaggg cgttggcgtc cacgccgccg
      301 gtcagcacct tgcccgaggc cggcaccacg gtgttgtagg cgcgggccag gcgggtgatg
      361 gagtccagca ggatcaccac gtccttcttc atttcgacca ggcgcttggc cttttcgatg
      421 accatctcgg ccacctgcac gtggcgcgtg gcgggttcgt cgaaggtcga ggccaccact
      481 tcgccgcgca cggtgcgctg catttcggtc acttcctcgg ggcgctcgtc gaccagcagc
      541 acgatcatca ccgcctcggg gtagttggtg gtgatggcgt gggcgatgtg ctgcatcatc
      601 accgtcttgc cggacttggg cggggacacg atcaggccgc gctggccatg gccgatgggc
      661 gcgaagatgt ccatgatgcg gccggtgagg ttttcctcgc tcttgatctc gcgttccagg
      721 cgcatgacgc gatccgggtg cagcggcgtc aggttctcga acatgatgcg gtgcttggcc
      781 gcttcgggca gtacgctgtt gatcttgtcg accttgacca gggcgaaata gcgctcgccg
      841 tccttgggca cgcgcacttc gccttcgatg gagtcgccgg tgtgcaggtt gaagcggcgg
      901 atctgcgagg gcgagatgta gatgtcgtcg gtgctggcca ggtaggaggt ctcgggcgag
      961 cgcaggaagc cgaagccgtc gggcagcact tcgagcacgc cgtcgccgaa gatctgctcg
     1021 ccctgcttgg cgcgccgctt catgatggcg aacatcagtt cttgcttgcg caggcggttg
     1081 gcgttttcga tttccaggcc tgcggccatt tccagcagct gcgatacgtg ctgggccttc
     1141 agttcattga gatgcattgc gatgggggat agatgaggga atgggttggc aggcagcgga
     1201 cgcgcccacg cggttgtctt acgtatggat gggaatgggg caattcagaa tctgggctgc
     1261 tctggccccg gcgtaatgcc ggtgtctggg cggcagcttt tacttatggc gacggccggg
     1321 gtgggctttg accctggccc tggccgggca ggcgcgggta ccagccggat ggctgattgc
     1381 ccgcgctggg catttttaca ttacccggta gtttacaacg taccgttcag gaatgcagtg
     1441 agttgcgatt tcgacagggc gccgaccttg gtggccgcgg ctttgccatc cttgaacagc
     1501 atcagggtgg ggatgccgcg gatgccatac ttggcagccg tgtccgggtt ggcgtcgacg
     1561 tcgaccttgg ccacggtgag gcggccggcg tattcgccgg cgacttcttc gaggatgggg
     1621 gcgatcatct tgcaggggcc gcaccaggct gcccagtagt cgaccagcac cggctggccg
     1681 gactggagca catcggtgtc gaagctggcg tcgctgacgt gtttgatttg ctcgctcatg
     1741 gaatttctcg ggggggctcg gattcgagag aagtgggttg ttacgcgaaa gcataccact
     1801 gtccataaca acagggttca atggttttgt gtaggatgtt gccgcgcgct gcactgcggc
     1861 gaggtggcgg gctggtcaga cgctgagcat cccagcccga gccgcgccag ccgtgcgccc
     1921 gtccgtaaaa tgtctgtttt tttccacaga acctggggat aacttggcca caccgcgtta
     1981 caccgaagcg tcgatccgcg ttctcaaggg gttggagccc gtgcgccagc gcccgggcat
     2041 gtacacccgc accgaaaatc ccctgcacat cgtgcaggag gtcatcgaca acgccgccga
     2101 cgaggccctg gcgggccatg gcaagcagat tcaggtcacg atgcatgccg atggcagcgt
     2161 ttccgtggaa gatgacggcc gcggcatccc ggtcggcctg caccccgaag agcacgcgcc
     2221 cgtggtcgag ctggtgttca cgcgcctgca tgccggcggc aagttcgaca aggccgcggg
     2281 gggcgcctat gccttttccg gcggcctgca cggcgtgggc gtgtcggtga ccaacgccct
     2341 ggccaccaag ctggaagtgg tggtctggcg cgacggcggc acgcaccgca tggtgttcgc
     2401 cggcggcgac gtggtcgaac ccgtcgcgcc cttcgatgcc ggcgtgaaga aaaaatccgg
     2461 cacccgcgtg cgcgtctggc ccgaccccaa gtacttcgac agcccggtca tcccgctggg
     2521 cgagctcacc cacctgctgc gcagcaaggc cgtgctgctg cccggcgtga aggtcacgct
     2581 caagaacgaa aagagcggcg acaccaagac ctggcagtac accgacggcc tgcgcggcta
     2641 cctggccgag gccctggccg gcgccgagct gatgattccc ttcttcgagg gcgagcagta
     2701 tgccgatgcc gaccacgaaa acttcgccga aggcgagggc gcccagtggg ccgtggcctg
     2761 gaccgaggac ggcaatgtgg tgcgcgagtc ctacgtcaac ctgatcccca ccccggccgg
     2821 cggcacgcac gagtcgggcc tgcgcgaagg cctgttcggc gcggtcaaga gctttgccga
     2881 cctgcacggc ctgctgccca agggcgtgaa gctgctgccc gaggacgtgt tcgcccgcgc
     2941 cagcttcgtg
//
LOCUS       DASWPO010000258        12125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1848831, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000258 DASWPO010000000
VERSION     DASWPO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848831"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VGC69_13560"
     CDS             <1..921
                     /locus_tag="VGC69_13560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0296351.1"
                     /translation="VLRDAQFGPVIRFGAGGPEAELSAWGGGMDLPPLNGYLARQLLA
                     RSRLWRRVVAPEVGGIAIEKLQHVLVQVSELVSELPDVEALEIDPLFIGETHLRAAGV
                     RMHLTREAAELPQLHGYPHMAIHPYPTRLVQVRRFDDGTPWVIRPIRPEDGESLQEFI
                     RGLSERSRYMRFVSMLRELTPRMLARYTQVDYHRELALVACTQVPNPAHRGHPREVII
                     GFAHYLRNADGRGAEYALVIGDDWQRQHLGGQLMQALIAEARDQGLEYIDGLVLAANR
                     PMLTLMTRLGFTNDADPEDPTMRRVWLNLK"
     gene            complement(1092..1763)
                     /locus_tag="VGC69_13565"
     CDS             complement(1092..1763)
                     /locus_tag="VGC69_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0296352.1"
                     /translation="MTTPLLDCLEIETAPRPTHAVIWLHGLGADANDFAPIVPELTQG
                     IEHAVRFVFPNAPVRPVTINGGMAMRAWYDILLPGLAHREDAQGIRASEEAVRALIAR
                     ENARGIATSNIVLAGFSQGSAMTLHTGLRLPEALAGMVALSGYLPLLELAAAERQPAN
                     ARTPIFLAHGVFDPVVVLPRAEASRAQLQALGCDVRWHTYPMPHSVCPPEIDDIRVFL
                     SEVIG"
     gene            complement(1760..2311)
                     /gene="yjgA"
                     /locus_tag="VGC69_13570"
     CDS             complement(1760..2311)
                     /gene="yjgA"
                     /locus_tag="VGC69_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY0296353.1"
                     /translation="MHAELPNGPDLSDDTADEGYDRPSKSQVKREMHALLDLGKELVE
                     LSPERLKQLPLAERLYESIREAQRTTSREGRRRQIHFVGKLMRNAPAEEIRAQLDIWN
                     NGSREATAAMHRLEALRDRLLADDDALTGLLQDHPGADVQHLRAVIRAARKEDQANAA
                     LQQGQEPQRKQYRALFQALKDLA"
     gene            2475..3839
                     /gene="pmbA"
                     /locus_tag="VGC69_13575"
     CDS             2475..3839
                     /gene="pmbA"
                     /locus_tag="VGC69_13575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEY0296354.1"
                     /translation="MVNSSSPLPVAANQARFSELVEKVLAYARRAGASDAVAEVSESL
                     GLSVSVRSNDIETVEQTRDRSLDLTVYAGQSRGSASTSDFSDEALRQTVEAAWHIARH
                     TAADPAAGLPDADLLATDHRDLALHYAWHIGTEDAAELALRAERAARAVDPRITNTDG
                     ASVSAYEGQFVMGNTRGFLGGYPYSRHSLSVAPIAGRGNNMQRDYWYTSERDAKLLAT
                     PEAVGRYAAERTLSRLSARRIATGRAPVLFEAPLALGLLGAFTQATSGGALYRKASFL
                     VDALGKPVFADHLDVAEDPHVRGGLGSSPFDDEGVRTQARQVVAGGVLEGYFLSSYTA
                     RKLGMVTTGNAGGSHNLSLTSRLTKKGDDFEAMLRKLGTGLLVTELIGQGVNYVTGDY
                     SRGAFGYWVENGRIQHAVQEITIAGNLADMFRHIVAVGADTITRGTKTTGSILIEQMS
                     IAGI"
     gene            3929..5062
                     /locus_tag="VGC69_13580"
     CDS             3929..5062
                     /locus_tag="VGC69_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296355.1"
                     /translation="MQRRSFLKQAGLGAAAGGAVLVAPAMAADAPSLKWRLASAFSEK
                     QDVLFGAAGVFAKYVSDATDGRFDIRPAPADDKAPASGVLDAVQKGTAEMGHAVSSLY
                     YDTNPALCFDAAVPFGLNARQMNAWMFEGDGLKLTRDLFKGYGIVNFPLGNTGGQMGG
                     WYKGEVKSIEAFKGLKIQASGIQALGIQASGLVGALLARMGAAPQALATADDVAKAFD
                     AGALDAVLGAGPYDDEKQGAPKAARFYYYPAWWSGSTQVSLYIGAKAWDSLPKPYQAI
                     VEAASRAAHLDMTARYDSQNAAALSRLVAGGAQLRAFPRSVLDAAWDAASAQYAELSD
                     KDPKFKALYDSHMGMRDELVSWFKLSEGAYDQYLASTLVFKKA"
     gene            5377..5805
                     /gene="rplM"
                     /locus_tag="VGC69_13585"
     CDS             5377..5805
                     /gene="rplM"
                     /locus_tag="VGC69_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY0296356.1"
                     /translation="MKTFVAKPHEVTRDWFVIDAKGKVLGRVASEVARRLRGKHKPEF
                     TPHVDTGDYIIIVNAADIVVTGNKAQDKKYFRHTTYPGGIRETNFTKMQERFPGRAIQ
                     KAVKGMLPKGPLGYAMIKKLKVYAGAEHPHTAQQPKTLDI"
     gene            5815..6207
                     /gene="rpsI"
                     /locus_tag="VGC69_13590"
     CDS             5815..6207
                     /gene="rpsI"
                     /locus_tag="VGC69_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY0296357.1"
                     /translation="MIGNWNYGTGRRKTSVARVFIKKGSGKIIVNGKPVDEFFARETG
                     RMIVRQPLELTGHVESFDIKVNVHGGGETGQAGAIRHGITRALIDYDAALKPSLSQAG
                     FVTRDAREVERKKVGFRKARRRKQFSKR"
     gene            6468..7535
                     /gene="argC"
                     /locus_tag="VGC69_13595"
     CDS             6468..7535
                     /gene="argC"
                     /locus_tag="VGC69_13595"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0296358.1"
                     /translation="MATNRIKVGIVGGTGYTGVELLRLLSQHPHVELAAITSRKEEGM
                     PVADMFPNLRGRVGLAFSTPENARLADCDVVFFATPHGVAMAQAKELLAAGTKIIDLA
                     ADFRLQDTAEFERWYKMPHSCPGILAEAIYGLVELNRESVARARVIGNPGCYPTTVIL
                     GLAPLIEGGKQLVDTQTLIADCKSGVSGAGRKAEVGTLFSEASDNFKAYGVAGHRHLP
                     EIIAQLEKLAGGKVPLTFVPHLVPMVRGMFSTIYARILPEARNTDFQKLFEERYANEH
                     FIDVMPAGSLPETRSVRASNTLRIAVQRPMQGGQPGDQLIVLVVQDNLVKGAAGQAVQ
                     NMNLMFGLPEAAGLDQVAILP"
     gene            7645..8016
                     /gene="erpA"
                     /locus_tag="VGC69_13600"
     CDS             7645..8016
                     /gene="erpA"
                     /locus_tag="VGC69_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY0296359.1"
                     /translation="MNAITETVNLQAPPPAPLLFTDSAAAKVKDLLLEEGNPELKLRV
                     FVQGGGCSGFQYGFTFDETVNEDDTVLDKSGVQLLVDPMSFQYLVGAEIDYKEDLEGS
                     QFVIRNPNATTTCGCGSSFSV"
     gene            complement(8289..9500)
                     /locus_tag="VGC69_13605"
     CDS             complement(8289..9500)
                     /locus_tag="VGC69_13605"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY0296360.1"
                     /translation="MSLPDTPGPALHGPGRLFIGLMSGTSLDGVDGVLMRFPAAGPPL
                     GGNAPSGGSKHDVRSVGAFPADDRPQTLAGVALPMPDGLRAELLALNTAGPDELERAA
                     RAANALARLYASAVERLLDETGLPASAVTAIGAHGQTVRHRPDQGFTIQLNAPALLAE
                     LTGIDVVADFRSRDVAAGGQGAPLVPPFHAAMFGGPAPRAILNLGGIANLTLLAPGQA
                     PRGFDTGPANVLLDAWCQRHAGQAYDADGRWAASGRADAGLLHALIASEPWLALPPPK
                     STGRDLFNAAWLDTRLAGWNLPPADVQATLQRFTAQTVADALNASLPAAQEVLVCGGG
                     ARNAGLMRDLADALQRPARATDEEGVAAQWMEALAFAWLAQMHVDGLPAGVPEVTGAR
                     GPRMLGALYPA"
     gene            complement(9506..10891)
                     /locus_tag="VGC69_13610"
     CDS             complement(9506..10891)
                     /locus_tag="VGC69_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0296361.1"
                     /translation="MTRRLISLFPKPATRGAPLIRRTLVVCAIGLFAAAAALGMVPNA
                     DPTPLPPLRLVSNPLPLTPGQMQVDPGASPAPFVSETRIRRGDTLSSVLQRLGLDDAR
                     LLAFLTQDSSARSIYKLYPGRAVQAATDDHGNLAWLHYVHTPSAKDDDDEQATTRMLV
                     IQPDQKGGYTAEERAEDTTVQTNVASGVIRSSLFAATDAADIPDGITLQMADILGAKI
                     DFLHGLRQGDQFRVVYETRSHEGRYAGPGRVLAVEFANNGKTYDAAWFSPDGAAGAYY
                     DFDGTSLRGAFLRTALKFTRISSTFGMRLHPLHKTWVGHKGVDYAAPAGTPIHATADG
                     VIDFAGWQNGYGNVVVIKHFGKYSTVYAHQSQIAAGIHQGTMVSQGQLIGYVGMTGWA
                     TGPHLHYEFRVDNQPVDPLSVDLPVARALEPGEHQAFVQMLTPYREQIRMLAELQQPG
                     RADPAGPIAAR"
     gene            11118..>12125
                     /gene="tyrS"
                     /locus_tag="VGC69_13615"
     CDS             11118..>12125
                     /gene="tyrS"
                     /locus_tag="VGC69_13615"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY0296362.1"
                     /translation="MSHRPAPEGATAAAAAYAITPEVEADLRVAKRGCDELLVESEFA
                     RKLARSRATGVPLRIKLGLDPTAPDIHLGHTVVLNKMRQLQDLGHTVIFLIGDFTSMI
                     GDPSGRNATRPPLTREQIESNAKTYYAQASLVLDPQRTEIRYNSEWSDPLGARGMIQL
                     ASRYTVARMMERDDFTKRFKGGIPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNL
                     LVGRELQKEYGQEPQCILTMPLLEGLDGVDKMSKSKHNYIGISEAPESMFGKIMSISD
                     TLMWRYYELLSFRTLDEIAALRAEIEAGRNPRDAKVLLGLEIVGRFHSAAAADGALAA
                     FE"
BASE COUNT         1988 a         4081 c         4124 g         1932 t
ORIGIN      
        1 gtgctgcgcg acgcccagtt cggtccggtc atccgtttcg gcgcgggcgg gcccgaggcc
       61 gagctgtcgg cctggggcgg cggcatggat ctgcccccgc tcaacggtta cctggcgcgc
      121 cagttgctgg cgcgcagccg tctgtggcgg cgcgtggtgg cgccggaagt gggcggcatc
      181 gccatcgaga agctgcagca cgtgctggtg caggtgtccg aactggtgtc cgaactgccg
      241 gacgtcgagg cgctggagat cgacccgctc ttcatcggcg agacgcacct gcgcgcggcc
      301 ggcgtgcgca tgcatctcac gcgcgaggcg gccgagctgc cgcagctgca cggctatccg
      361 cacatggcca tccatccgta tcccacgcgg ctggtgcagg tgcggcgctt cgacgacggc
      421 acgccctggg tcatccggcc catccgcccc gaggacggcg aatcgctgca ggagttcatc
      481 cgcgggctgt cggagcgctc gcgctacatg cgcttcgtgt ccatgctgcg cgaactcacg
      541 ccgcgcatgc tggcgcgcta cacccaggtc gactaccacc gcgagctggc gctggtggcc
      601 tgcacgcagg tgcccaatcc ggcgcaccgc ggccatccgc gcgaggtcat catcggcttc
      661 gcccattacc tgcgcaatgc cgacgggcgc ggcgccgaat acgccctggt catcggcgac
      721 gactggcagc gccagcacct gggcggccag ctgatgcagg cgctgattgc cgaagcgcgc
      781 gaccaggggc tggaatacat cgacggcctg gtgctggccg ccaaccggcc catgctcacg
      841 ctgatgaccc gcctgggctt caccaacgac gccgatccgg aagacccgac catgcgccgg
      901 gtgtggctga acttgaaatg acgcgaactt gaagcaacgc gtctttagaa cgacgtggag
      961 cttgaagccc cttgagccga ggaccccgga gcaatccgaa gcgcagcccc caccaggggc
     1021 gagcatcgga attgcgcagg gcagcctcgg cgcctcaaga actcaaacgc cgccacgccg
     1081 caaagaaact ctcagccaat cacttccgag agaaacaccc ggatatcgtc gatctccggc
     1141 gggcagaccg aatgcggcat gggataggtg tgccagcgca catcgcagcc cagcgcctgg
     1201 agctgcgccc gcgatgcctc ggctcgcggc agcaccacca ccggatcgaa gacgccatgg
     1261 gccaggaaga tcggcgtgcg cgcattggcc ggctgccgtt cggcggcggc cagctcgagc
     1321 agcggcaggt agcccgacag ggccaccatg ccggccagcg cttcgggcag gcgcagcccg
     1381 gtgtgcagcg tcatggcgct gccctgcgag aagccggcca gcacgatgtt cgacgtggcg
     1441 atgccgcggg cgttctcgcg ggcaatcaat gcgcggaccg cctcttcgct ggcgcggatg
     1501 ccctgggcat cttcacggtg cgccaggccc ggcagcagga tgtcgtacca ggcgcgcatg
     1561 gccatgccgc cgttgatcgt caccggccgc accggcgcgt tgggaaatac gaagcgcacg
     1621 gcgtgctcga tgccctgcgt cagctcgggc acgatgggcg cgaaatcgtt ggcgtcggcg
     1681 cccagtccgt gcagccagat cacggcgtgc gtggggcggg gggcggtctc gatctcgagg
     1741 cagtcaagca ggggagtcgt catgccagat ccttcaaagc ttgaaacaag gctcgatatt
     1801 gcttgcgctg cggttcctgg ccttgctgca gggcggcgtt ggcctggtct tccttgcggg
     1861 cggcgcggat cacggcgcgc aggtgctgca cgtcggcgcc ggggtggtcc tgcagcagtc
     1921 cggtgagcgc gtcgtcgtcc gccagcaggc ggtcgcgcag ggcttccagg cgatgcatgg
     1981 cggccgtggc ctcgcgcgag ccgttgttcc agatgtcgag ctgggcgcgg atttcctcgg
     2041 cgggggcatt gcgcatcagc ttgcccacga agtggatctg gcgccggcgg ccttcgcggc
     2101 tggtggtgcg ctgcgcctcg cggatggact cgtacaggcg ctcggccagc ggcagctgct
     2161 tcaggcgttc gggagaaagc tccaccagct ccttgcccag gtcgagcagg gcatgcatct
     2221 cgcgcttgac ctgcgatttg ctgggccggt cgtagccttc gtctgcggtg tcgtcggaaa
     2281 ggtctggtcc gttggggagc tcggcgtgca taggaaagcg gggggaggga ggcgggaaaa
     2341 ccggggtaac ggcttgggca gcaaaacggg cggtcaaaaa ccggcaatca aaacaggcca
     2401 tcgcgcgcct gcgcggccag ccggccggct gcggacttgg ggctatgata accgcctcag
     2461 caaactggac agcaatggtc aattcctctt ctcccctgcc ggtggccgcc aaccaggcgc
     2521 gctttagcga actcgtcgaa aaagtgctgg cctacgcgcg ccgggcgggc gccagcgatg
     2581 ccgtggccga agtctccgaa agcctgggcc tgtcggtctc ggtacgcagc aacgacatcg
     2641 aaaccgtcga gcagacgcgc gaccgctcgc tcgacctcac cgtctacgcc ggccagagcc
     2701 gcggctcggc atccacttcc gacttttccg acgaggccct gcgccagacg gtcgaggccg
     2761 cctggcacat cgcccgccat accgcggccg acccggccgc cggcctgccc gacgccgacc
     2821 tgctggccac cgaccaccgc gacctggccc tgcactacgc ctggcacatc ggcaccgaag
     2881 acgccgccga gctggcgctg cgcgccgaac gcgccgcgcg cgcggtcgac ccgcgcatca
     2941 ccaataccga cggcgcctcg gtgagcgcct acgaaggcca gttcgtcatg ggcaatacgc
     3001 gcggcttcct gggcggctat ccgtattcgc gccacagcct gtcggtggcg cccatcgccg
     3061 ggcgcggcaa caatatgcag cgcgactatt ggtacacctc cgagcgcgac gccaagctgc
     3121 tggccacgcc cgaggcggtc ggccgctacg ccgccgagcg cacgctgtcg cgcctgtcgg
     3181 cccgccgcat tgccaccggc cgcgcgccgg tgctgttcga ggcgccgctg gcgctgggcc
     3241 tgttgggcgc cttcacccag gccaccagcg gcggcgcgct ctaccgcaag gccagcttcc
     3301 tggtcgacgc gctgggcaag ccggtgttcg ccgatcacct cgacgtggcc gaagacccgc
     3361 acgtccgggg cggcctgggc agctcgccct tcgacgacga aggcgtgcgc acgcaggccc
     3421 gccaggtcgt cgcgggcggc gtgctcgaag gctatttcct gtcctcgtac acggcccgca
     3481 agctgggcat ggtcaccacc ggcaatgccg gcggctcgca caacctgtcg ctcacctcgc
     3541 gcctgacgaa gaaaggcgac gatttcgagg ccatgctgcg caagctgggc acgggcctgc
     3601 tggtcaccga actcatcggc cagggcgtga actacgtcac gggcgattat tcgcgcggcg
     3661 ccttcggcta ttgggtcgag aacggccgca tccagcatgc cgtgcaggaa atcaccatcg
     3721 ccggcaacct ggccgacatg ttccgccaca tcgtcgcggt gggcgccgac accatcacgc
     3781 gcgggaccaa gaccacgggg tccatcctga tcgaacagat gtcgatcgca ggcatctagc
     3841 ccgcgcgccg tccgggttac atcgtccgga ttacatcgtc cattcatcag gccgccctcc
     3901 acagcatgca cttcgggaga tgaaaaacat gcaacgtcgc tcattcttga agcaggccgg
     3961 cctgggtgcc gccgcgggtg gcgccgtcct ggtcgcgccg gccatggcgg ccgatgcgcc
     4021 ctcgctcaaa tggcgcctgg cctcggcctt ttccgaaaag caggacgtgc tgttcggcgc
     4081 ggccggggtg ttcgccaagt atgtgtccga cgccacggac ggccgtttcg acatccggcc
     4141 cgcgccggcc gacgacaagg cgccggcctc gggcgtgctg gatgcggtgc agaagggcac
     4201 ggccgagatg gggcatgccg tatcgtccct gtattacgac accaatccgg ccctgtgctt
     4261 cgatgcggcc gtgcccttcg gcctgaacgc gcggcagatg aatgcctgga tgttcgaggg
     4321 tgacgggctc aagctcacgc gcgatctctt caagggctac ggcatcgtca atttcccctt
     4381 gggcaatacc ggcggccaga tgggcggctg gtacaagggc gaggtcaagt ccatcgaggc
     4441 gttcaagggc ctgaagatcc aggcgtcggg catccaggcg ctgggcatcc aggcgtcggg
     4501 tctggtgggg gcgttgctgg cccgcatggg cgccgcgccg caggccttgg cgaccgccga
     4561 cgatgtggcc aaggccttcg acgcgggcgc gctggacgcc gtcctgggcg ccggccccta
     4621 cgacgacgag aagcaggggg cgcccaaggc ggcccgtttc tactattacc cggcctggtg
     4681 gtcgggcagc acgcaggtct cgctctatat cggcgccaag gcctgggaca gcctgcccaa
     4741 gccgtaccag gccatcgtcg aggcggccag ccgcgcggcg cacctggaca tgacggcgcg
     4801 ctacgacagc cagaacgccg cggccttgag ccgcctggtg gcgggcggcg cgcaactgcg
     4861 cgccttcccg cggtcggtgc tggacgcggc ctgggatgcc gcgtcggccc agtacgccga
     4921 actgtcggac aaggatccga agttcaaggc gctttacgac agccacatgg gaatgaggga
     4981 cgagcttgtt tcctggttca agctttcgga aggggcttat gaccagtatc tggcttctac
     5041 gctggtgttc aagaaggcct gaaacatggg ttcttgggcc gtccgggatg tccagggact
     5101 ggcgcccgga cacgtcggtg cggctcgggc gcgccatcag gcgccctcgg cccctgcggg
     5161 gctgcccttc ctcctactgt ccgggcgcca gcccctggac atcccggact ccacctgttt
     5221 ttcatagcgg cttgcgcaaa aaatgcacag gtgctattgt gttgggctct ttcgcatctt
     5281 ttgcacgggc ggttaacaac gcttaggccg agagcgaggg aaaacccggg cccaggattt
     5341 tctggcgatc cggaactttg aaatctagga accatcatga agacctttgt ggccaagccg
     5401 catgaagtca cgcgtgactg gtttgtgatc gacgccaagg gcaaggtcct cggtcgtgtg
     5461 gccagcgaag tcgcacgccg tctgcgtggc aagcacaaac ctgaattcac gccgcacgtt
     5521 gacaccggtg attacatcat catcgtcaac gctgccgata tcgtcgttac cggtaacaag
     5581 gcgcaagaca agaagtactt ccgccacacc acctacccgg gcggtatccg cgaaaccaat
     5641 ttcacgaaaa tgcaagagcg ttttcctggc cgcgccatcc agaaggccgt caagggcatg
     5701 ctgcccaagg gtcccctggg ctacgcgatg atcaagaagc tgaaggtgta cgccggtgcc
     5761 gagcatccgc ataccgccca gcagcccaag acgctggaca tctaaggaaa cgccatgatc
     5821 ggtaactgga actacggaac cggccgtcgc aagacctcgg tggcccgcgt tttcatcaag
     5881 aagggttcgg gcaagatcat cgtcaacggc aagcccgtcg acgagttctt cgctcgcgaa
     5941 accggccgca tgatcgtgcg ccagccgctg gaactgaccg gccatgtcga aagcttcgac
     6001 atcaaggtca atgtgcacgg cggcggtgaa accggccagg ccggcgcgat ccgccacggc
     6061 atcacccgcg cgctgatcga ctacgacgcg gcgctcaagc cctcgctgtc gcaagccggc
     6121 ttcgtcaccc gcgacgcccg cgaagtcgaa cgcaagaagg tcggcttccg caaggcgcgc
     6181 cggcggaagc agttcagcaa gcgttaatac gctgcaaccc cagaaaggcc gcgcaagcgg
     6241 cctttttgtt tgagatgccg cagcatgact gtcttggccc agggggccgg acagtaggag
     6301 gaagggcagc cccgcagggg ccgaggcccc gtcgtcttgg ggccgagccg cgccgacgtg
     6361 tccggccccc tgggccaaga cagtcatgcg tgttaagaac tcagaaacag ttttacgcga
     6421 tactaaaata ctcagtcatc gcaccctaca cttcctggat gaacaccatg gcaacgaacc
     6481 gcatcaaggt cggcatcgtc ggcggcaccg gctacaccgg cgtcgagctg ctgcgcctct
     6541 tgtcgcagca cccccacgtg gagctcgccg ccatcacctc ccgcaaggaa gagggcatgc
     6601 cggtcgccga tatgttcccc aatctgcgcg gccgcgtcgg cctggccttc tccacgcccg
     6661 agaacgccag gctcgccgac tgcgacgtcg tgttcttcgc caccccgcac ggcgtggcca
     6721 tggcgcaggc caaggaactg ctggccgccg gcaccaagat catcgacctg gccgccgact
     6781 tccgcctgca ggacaccgcc gaattcgagc gctggtacaa aatgccccac agctgcccgg
     6841 gcatcctggc cgaggccatc tacggcctgg tcgagctcaa tcgcgaatcg gtcgcccgcg
     6901 cgcgcgtcat cggcaacccc ggctgctacc ccaccacggt catcctggga ctggccccgc
     6961 tgatcgaggg cggcaagcag ctggtcgata cgcaaaccct gatcgccgac tgcaaatcgg
     7021 gcgtgtccgg cgccggccgc aaggccgagg tgggcacgct gttttccgag gcctccgaca
     7081 acttcaaggc ctacggcgtg gccggccacc gccacttgcc cgaaatcatc gcccagctcg
     7141 agaagctggc cggcggcaag gtgccgctga ccttcgtgcc gcacctggtg cccatggtgc
     7201 gcggcatgtt ctcgacgatc tacgcccgca tattgcccga ggcgcgcaac accgatttcc
     7261 agaagctctt cgaagaacgc tacgccaacg agcacttcat cgacgtcatg ccggccggca
     7321 gcctgcccga gacccgctcg gtgcgcgctt ccaacaccct gcgcatcgcg gtgcagcgcc
     7381 ccatgcaggg tggccagccc ggcgaccagc ttatcgtcct ggtcgtgcag gacaacctgg
     7441 tcaagggcgc cgccggccag gccgtgcaga acatgaacct gatgttcggc ctgccggagg
     7501 cggccggcct ggaccaggtc gctattctgc cgtaagggtt atcgccgtcc gggccagggg
     7561 gcctgggtta tagtgaacat gtccggggca gcgagtctgc ccatatttgg ccgatgcgca
     7621 cagcgcccgg ccaggagtcg ccaaatgaac gccattaccg aaaccgtcaa tctgcaggcc
     7681 ccgccgcctg cgcccttgct tttcaccgac tcggccgccg ccaaggtgaa ggacctgctg
     7741 ctcgaagaag gcaaccccga gctcaagctg cgcgtgttcg tgcagggcgg gggctgttcg
     7801 ggcttccagt acggttttac cttcgacgaa accgtcaacg aagacgacac cgtgctcgac
     7861 aagagcggcg tgcagctgct ggtcgatccc atgagcttcc agtacctggt cggcgccgag
     7921 atcgactaca aggaagacct cgaaggctcg cagttcgtca tccgcaatcc caacgccacc
     7981 accacctgcg gctgcggctc gtcgttctcg gtgtaagcgt cgatcgcgga taccgcgaga
     8041 atacagggaa gcccgtgcat ggattgcgcg ggctttttgc tttgggggtg ttgtctgagt
     8101 tcttaatacg tccgtcggtt ggcgcggaca ccccggggct tcgctgtgcc gggctcgggc
     8161 gcataccggc gccctcggcc ccctgcgggg gctgccccgt cacagaggcg ccccggggtg
     8221 tccgcgccaa ccggcggact gcagagcgtt catgcagccg cggcgccgac tccctccgac
     8281 tccctagcct aggctgggta cagggcgccg agcatgcggg ggccgcgggc gcccgtcact
     8341 tccgggacgc cggccggcaa gccatcgacg tgcatctgcg ccagccaggc gaaggccagc
     8401 gcttccatcc attgcgcggc cacgccttct tcgtcggtgg cgcgcgccgg gcgctgcaag
     8461 gcatcggcca ggtcgcgcat caatccggcg ttgcgcgcgc cgccgccgca gaccagcact
     8521 tcctgcgcgg cgggcaatga tgcattcagg gcatcggcca ccgtctgagc ggtgaagcgt
     8581 tgcagcgtgg cctgcacgtc ggcgggaggc aggttccagc ccgccaggcg ggtatcgagc
     8641 caggcggcgt tgaacaggtc gcggccggtg gatttgggcg gcggcaaggc cagccagggt
     8701 tcgctggcga tgagggcgtg cagcaggccg gcatcggccc ggccgctggc ggcccagcgt
     8761 ccgtccgcgt cataggcctg gccggcatgg cgctggcacc aggcatcgag cagcacattg
     8821 gccgggccgg tatcgaagcc gcgcggcgcc tggccggggg ccagcagcgt caggttggcg
     8881 atgccgccca ggttgaggat ggcgcgcggg gcggggccgc cgaacatggc cgcatgaaaa
     8941 ggcggcacca gcggagcgcc ctggccgccc gcggccacgt cccggctgcg gaagtcggcc
     9001 accacgtcga tcccggtgag ttcggccagc agcgccgggg cgttgagctg gatggtgaaa
     9061 ccctggtcgg ggcggtgccg cacggtttgc ccatgcgcgc cgatggcggt gacggccgat
     9121 gcgggcaggc ccgtttcgtc cagcaggcgt tctacggccg aggcatacag ccgcgccagc
     9181 gcattggccg cgcgcgcggc gcgttcgagt tcgtcggggc cggcggtatt gagcgccagc
     9241 agctcggcgc gcaggccgtc gggcatgggc agggccacgc cggccagggt ctgcggccgg
     9301 tcgtcggcgg ggaaggcccc cacgctgcgc acgtcgtgct tgctgccccc cgagggggcg
     9361 ttcccaccca ggggcggccc ggcggcggga aagcgcatca gcacgccgtc caccccatcc
     9421 aggctggtgc cggacatcag gccgatgaac aagcggcccg ggccgtgcag ggccgggccg
     9481 ggggtatcgg gcagggacat ggcggtcaac gcgcggcgat ggggccggcc gggtcggccc
     9541 ggcccggctg ctgcagctcg gccagcatgc ggatctgctc gcggtagggc gtcagcatct
     9601 gcacgaaggc ctggtgttca ccgggctcga gcgcgcgggc cacgggcagg tcgaccgaca
     9661 gcgggtcgac gggctggttg tcgacgcgga attcgtaatg caggtgcggt ccggtggccc
     9721 agcccgtcat gcccacgtag ccgatcagct ggccctgcga caccatggtg ccctggtgga
     9781 tgcccgcggc gatctggctc tggtgggcgt acacggtgga gtacttgccg aagtgcttga
     9841 tgacgaccac gttgccgtag ccgttctgcc agccggcgaa gtcgatcacc ccgtcggccg
     9901 tggcatggat gggcgtgccc gccggcgcgg cgtagtccac gcccttgtgg cccacccagg
     9961 tcttgtgcag cgggtgcagg cgcatgccga aggtcgagct gatgcgggtg aacttgagcg
    10021 cggtgcgcag gaaggcgccg cgcaggctcg tgccgtcgaa gtcgtagtag gcgcccgccg
    10081 cgccgtcggg gctgaaccat gcggcgtcgt aggtcttgcc gttgttggcg aattccacgg
    10141 ccagcacgcg gcccgggccg gcatagcggc cttcgtgcga gcgcgtctcg tacaccacgc
    10201 ggaactggtc gccctggcgc aggccgtgca ggaagtcgat cttggcgccg aggatgtcgg
    10261 ccatctgcag ggtgatgccg tcggggatgt cggccgcgtc ggtggcggca aacagcgacg
    10321 agcggatcac gccggaagcc acattggttt gcaccgtggt gtcctcggcg cgctcttcgg
    10381 ccgtgtagcc gcccttctgg tcgggctgga tcaccagcat gcgggtcgtg gcctgctcgt
    10441 cgtcatcgtc cttggcgctg ggcgtgtgca cgtagtgcag ccaggccagg ttgccgtggt
    10501 cgtcggtggc ggcctgcacc gcgcggccgg ggtagagctt gtagatgctg cgcgcgctgc
    10561 tgtcctgggt caggaaggcc agcagcctgg cgtcgtccag ccccaggcgc tgcagcacgc
    10621 tcgacagcgt atcgccgcgg cggatgcggg tttcgctgac gaagggggcc gggctggcgc
    10681 cggggtcgac ctgcatctgc ccgggcgtca gcggcagggg gttgctgacc agccgcaggg
    10741 gcggaagcgg ggtggggtcg gcgttgggca ccatgcccag cgcggcggcg gcggcaaaaa
    10801 ggccgatggc gcagacgact agtgtgcggc ggatcagggg cgcgccgcgg gtggcgggtt
    10861 tcggaaagag agagataagg cggcgggtca cttgggaacg atgggcgtgt tgctggattg
    10921 ctacaattgc gtaggcaaca gggggcaagc cgggctcggc ctgttgcttg cgattagcgc
    10981 agtatgctaa tcgaatcgtc ccggaatttc gaccgttttc cgccttgctg ttacaacctt
    11041 ttgtggcacc aagtacatga aaccactaga gtttgtggtt tttgatttct tacaccttct
    11101 cggtttttta gcatttcatg tctcatagac cagcccctga aggggccact gcggcggccg
    11161 ccgcctacgc catcactccc gaggtcgagg ccgatctgcg cgtcgccaag cgcggctgcg
    11221 acgaactgct ggtcgaatcc gaattcgccc gcaagctggc ccgcagccgg gccacgggcg
    11281 tgcccctgcg catcaagctg gggctggacc cgaccgcgcc ggacatccac ctgggccaca
    11341 cggtggtgct caacaagatg cgccagctgc aggacctggg ccataccgtc atcttcctga
    11401 tcggcgactt cacctcgatg atcggcgacc ccagcggccg caacgccacc cgcccgccgc
    11461 tcacgcgcga gcagatcgag agcaacgcca agacctatta cgcccaggcc agcctggtgc
    11521 tcgaccccca gcgcaccgag atccgctaca actccgagtg gtccgacccg ctgggcgcgc
    11581 gcggcatgat ccagctggcc tcgcgctaca ccgtggcccg catgatggag cgcgacgact
    11641 tcaccaagcg cttcaagggc ggcattccca tctcggtgca cgaattcctc tacccgctga
    11701 tgcagggcta cgactcggtg gcgctcaagt ccgacctgga gctgggcggc accgaccaga
    11761 agttcaacct gctggtgggc cgcgagctgc agaaagaata cggccaggag ccgcagtgca
    11821 tcctgaccat gccgctcctg gaaggcctgg acggcgtcga caagatgtcc aagtccaagc
    11881 acaactacat cggcatctcc gaggcgcccg aatccatgtt cggcaagatc atgtcgatct
    11941 ccgacacgct gatgtggcgc tactacgagc tgctgtcgtt ccgcacgctc gacgagatcg
    12001 ccgccttgcg cgccgagatc gaggccggcc gcaatccgcg cgacgccaag gtgctgctgg
    12061 gcctggagat cgtgggccgc ttccactccg cggccgcggc cgacggcgcc ctggcggcct
    12121 tcgag
//
LOCUS       DASWPO010000259         4031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2829451, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000259 DASWPO010000000
VERSION     DASWPO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829451"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /gene="rpsD"
                     /locus_tag="VGC69_13620"
     CDS             <1..432
                     /gene="rpsD"
                     /locus_tag="VGC69_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY0296363.1"
                     /translation="MYGVLEKQFRKYFAEAERVRGNTGETLIKLLESRLDNVVYRMGF
                     GSTRAEARQLVSHRAVELNGHTADVASIIVKAGDVVSIREKAKKQGRIKESLDLATSI
                     GIPQWVEVDTSKMTGTFKTAPDRADVARDVNESMVVELYSR"
     gene            660..1649
                     /gene="rpoA"
                     /locus_tag="VGC69_13625"
     CDS             660..1649
                     /gene="rpoA"
                     /locus_tag="VGC69_13625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY0296364.1"
                     /translation="MSTQGFLKPRSIEVEPVGPNHAKIVMEPFERGYGHTLGNALRRI
                     LLSSMTGYAPTEVQMTGVVHEYSTIAGVREDVVDILLNLKGVAFKLHNRDDVTLVLRK
                     SGAGEVRASDIELPHDVEIINPDHIICSLTEAGKLEMQIKVEKGRGYVPGNVRALSED
                     RGHTIGRIVLDASFSPVRRVSYAVESARVEQRTDLDKLVLDIETNGVISPEEAVRQSA
                     RILMDQISVFAALEGAAETYGSEVRGSAPQIDPVLLRPVDDLELTVRSANCLKAENIY
                     YIGDLIQRTENELLKTPNLGRKSLNEIKEVLAARGLTLGMKLENWPPLGLERP"
     gene            1835..2224
                     /gene="rplQ"
                     /locus_tag="VGC69_13630"
     CDS             1835..2224
                     /gene="rplQ"
                     /locus_tag="VGC69_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY0296365.1"
                     /translation="MRHGNGLRKLNRTSSHRLAMFRNMAVSLITHEAIKTTLPKAKEL
                     RRVIEPLITLGKQPTLANKRLAFARLRDRDAVLKLFAEIGPRYATRNGGYTRVLKMGF
                     RQGDNAPMAFMELVDRPESQEAEASAE"
     gene            2345..2686
                     /gene="cutA"
                     /locus_tag="VGC69_13635"
     CDS             2345..2686
                     /gene="cutA"
                     /locus_tag="VGC69_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY0296366.1"
                     /translation="MVMNDDDVVLVLSSVPEASLAERIAHVLVEGGLAACVNVGAPMV
                     SVYAWQGRIERTGEIPLVIKTTRARQEEVIQALAKLHPYEVPEVLVVPVQDGLDSYLG
                     WVRRQTQVQWH"
     gene            2689..>4031
                     /gene="dsbD"
                     /locus_tag="VGC69_13640"
     CDS             2689..>4031
                     /gene="dsbD"
                     /locus_tag="VGC69_13640"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEY0296367.1"
                     /translation="MSTNRSWWLALCGLLAALLAWGGAAAQSEADFLAPEQAFVLEAK
                     MAAPDALVLHYRVAQGYYLYRERFALAPAEGQAPWLGEPSYPAGEIKHDPVFDKDMEV
                     YHHDLDVRVPLKPGAAGPWVLTVTNQGCADAGLCYPPMDHAVTLSPVAGGYAVAGVDL
                     PQGQAGVSVPAARAALAAGNANAGPALGALLQAGDTGLAQALGGLGWLKTAGVFLALG
                     VLLSFTPCVLPMVPIVSALALGGAARRTRWRGLGLAAAYVLGMSVVYTALGVAAGLSG
                     AGLAAWLQTPWALAIFALLLVLLALAMFDVYTFQMPAQAQAGLTGWANRMPGGRFTGA
                     LLMGAASALIVGPCVAAPLAGALLYISRTGDVLLGGSALFALAWGMGVPLLAVGASAG
                     ALLPRTGPWMDAVKRLFGMLLLATAWWMLTPVLPGWTQMLGWAFLAVVSAVMLRA"
BASE COUNT          682 a         1357 c         1329 g          663 t
ORIGIN      
        1 atgtacggcg tcctggaaaa gcaattccgc aagtacttcg ctgaagccga gcgcgtccgc
       61 ggcaacaccg gtgaaacgct gatcaagctg ctcgaatcgc gcctggacaa cgtcgtctac
      121 cgcatgggct tcggctcgac ccgcgccgag gctcgccaac tggtcagcca ccgcgccgtc
      181 gaactgaacg gccacaccgc cgacgtcgcc tcgatcatcg tcaaggctgg cgacgtggtc
      241 tcgatccgcg aaaaggccaa gaagcaaggc cgcatcaagg aatcgctcga cctggccacc
      301 agcatcggca tcccccaatg ggtggaagtc gacacctcca agatgaccgg caccttcaag
      361 acggcgcccg atcgcgccga cgtggcccgc gacgtcaacg agtcgatggt cgtcgaactg
      421 tactcgcgtt aatacggcga tcgtcgcatg agcctcgcgg catggaaatg tcgcgagccg
      481 aggaccccgg aggaatccga agcgcagggg gcattgcagc ccccgagcat cggaattccg
      541 cagggcagcc tcggcggctc aagaaagatt tcatccactg cacgacagct ttcccccacc
      601 tgccatccat cagccttatc ggtgtaacga gccgagggta ttgaaaagga tctatccaga
      661 tgtccactca aggtttcctg aagccgcgtt ccatcgaagt cgaacccgta ggccccaatc
      721 acgccaagat cgtgatggag cccttcgaac gcggctacgg ccacacgctg ggcaacgccc
      781 tgcgccgcat cctgctgtcg tcgatgaccg gctatgcgcc caccgaagtc cagatgaccg
      841 gcgtggtgca tgagtactcg accatcgccg gcgtgcgtga agacgtggtc gacattctgc
      901 tgaacctcaa gggcgtggcg ttcaagctgc acaaccgcga cgacgtgacc ctggtgctgc
      961 gcaagagcgg cgccggcgaa gtgcgcgcca gcgacatcga actgccccac gacgtcgaga
     1021 tcatcaatcc cgaccacatc atctgcagcc tgaccgaagc cggcaagctg gaaatgcaga
     1081 tcaaggtcga gaagggccgc ggctatgtgc cgggcaatgt gcgcgcgctg tcggaagacc
     1141 gcggccacac catcggccgc atcgtgctgg acgcctcgtt ttcccccgtg cgccgcgtga
     1201 gctacgccgt cgaaagcgcc cgcgtcgagc agcgcaccga cctggacaag ctggtcctgg
     1261 acatcgaaac caacggcgtg atctcgcccg aggaagccgt gcgccagtcg gcccgcatcc
     1321 tgatggacca gatctccgtc ttcgccgccc tggaaggcgc cgccgaaacc tacggcagcg
     1381 aagtccgcgg cagcgccccg cagatcgacc ccgtgctgct gcgcccggtc gacgacctgg
     1441 agctgacggt gcgttcggcc aactgcctga aggccgaaaa catctactac atcggcgacc
     1501 tgatccagcg caccgaaaac gagctgctca agaccccgaa cctgggccgc aagtcgctca
     1561 acgaaatcaa ggaagtcctg gccgcacgcg gcctgaccct gggcatgaag ctcgagaact
     1621 ggccccccct cggcctcgag cgcccgtaat agacccaccc ccgaagcgcc tgtggcgctt
     1681 ccccctcaag ggggcgccgc tggcagaccg gcaaagccgg atctgcgtcg gcccaggcag
     1741 acctcatttt tgcaccggcc cgcgaacttt cgatagaaga gccggataac ccgatgactc
     1801 acaaggtcat cactagattc aaaggaaata caccatgcgt cacggtaatg gtttgcgcaa
     1861 gctcaaccgc accagcagcc accgtcttgc catgttccgc aacatggccg tctcgctgat
     1921 cacccacgag gccatcaaga ccacgctgcc caaggccaag gaactgcgcc gcgtcatcga
     1981 acccctgatc accctgggca agcagcccac cctggcgaac aagcgcctgg ccttcgcccg
     2041 cctgcgcgat cgcgatgccg tcctcaagct gttcgccgag atcggcccgc gctacgccac
     2101 ccgcaacggc ggctataccc gtgtgctgaa gatgggcttt cgccagggcg acaatgcgcc
     2161 catggccttc atggaactgg tcgatcgtcc ggaatcccag gaagccgaag ccagcgccga
     2221 gtaatcgcgc ggcgcgctgc acgagacggg ccgggttgca taaacccggc ccgttttttc
     2281 ttatggatcg cccgggcggt cttaaaatgg cggttccggc caaacctggc ttcgggagcc
     2341 ttccatggta atgaacgacg acgatgtggt gctggtgctc agcagcgtgc ccgaggcctc
     2401 gctggccgag cgcattgccc acgtgttggt cgaaggcggc ctggcggcct gcgtcaatgt
     2461 gggcgcgccc atggtgtcgg tctatgcctg gcagggcagg atcgagcgaa ccggggaaat
     2521 tccgctcgtc atcaagacga cccgtgcccg gcaggaagaa gtgatacagg ccctggccaa
     2581 gctccatccc tatgaagtgc ccgaagtgct ggtggtaccc gtgcaggacg gcctggattc
     2641 gtacctgggc tgggtacggc ggcagacaca ggtgcaatgg cattgacgat gtcgacgaat
     2701 cgatcctggt ggctggccct gtgcggcttg ctggcggccc tgctggcctg gggcggggcc
     2761 gcggcgcaga gcgaggccga tttcctggcg cccgagcagg ccttcgtgct cgaggcgaag
     2821 atggccgcgc cggatgcgct ggtgctgcac tatcgcgtgg cccagggcta ttacctttac
     2881 cgcgaacgct tcgcgctggc gccggccgaa ggccaggcac catggctggg cgagccgtcc
     2941 taccccgcgg gtgaaatcaa gcacgacccc gtcttcgaca aggatatgga ggtctaccac
     3001 cacgacctgg acgtgcgcgt gccgctcaag ccgggcgcgg cggggccgtg ggtgctgacc
     3061 gtgaccaacc agggctgcgc cgatgcggga ctgtgctatc cgcccatgga tcatgccgtg
     3121 acgctgtcgc ccgttgcggg cggctatgcc gtggcgggcg tggatttgcc gcaggggcag
     3181 gcgggcgtgt cggtgcccgc ggcgcgcgcg gctctcgcgg cgggcaatgc aaatgccgga
     3241 ccggcgctcg gcgccttgtt gcaggccggc gataccggcc tggcccaggc cctgggcgga
     3301 ctgggttggc tgaagacggc gggcgtgttc ctggcgctgg gcgtgctgct gtcgttcacg
     3361 ccctgcgtgc tgcccatggt tcccatcgtg tcggccctgg cgctgggcgg cgcggcgcgg
     3421 cgcacgcgct ggcgcggcct gggcctggcg gcggcctatg tgctgggcat gtcggtggtc
     3481 tacaccgcgc tgggcgtggc cgcgggcctc tccggcgcgg gcctggccgc ctggctgcag
     3541 acgccctggg cgctggcgat attcgcgctg ctcctggtcc tgctggccct ggccatgttc
     3601 gacgtctaca ccttccagat gccggcgcag gcgcaggccg gcctgaccgg ctgggccaat
     3661 cgcatgcccg gcggccgctt cacgggggcc ctgctgatgg gcgccgcgtc ggccctgatc
     3721 gtggggcctt gcgtggccgc gccgctggcg ggcgccttgc tgtacatctc gcgcaccggc
     3781 gacgtgctgc tgggcggttc ggcgctcttc gcgctggcct ggggcatggg cgtgcccctg
     3841 ctggccgtgg gcgcctcggc aggggccctg ctgccgcgca cggggccgtg gatggatgcg
     3901 gtcaagcgtc ttttcggcat gctgctgctg gccacggcct ggtggatgct cacgccggtg
     3961 ctgccgggct ggacgcagat gctgggctgg gcttttctcg ccgtcgtgag cgcggtcatg
     4021 ctgcgggcct t
//
LOCUS       DASWPO010000260         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_865907, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000260 DASWPO010000000
VERSION     DASWPO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865907"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGC69_13645"
     CDS             complement(<1..534)
                     /locus_tag="VGC69_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HEY0296368.1"
                     /translation="MTTSAIPSSSNAGTITSLGVGSSLDLQGILDSLQAADNQSLTVI
                     QNRINSYQTQISAYGTLQSAVQGVASAAQTLGQASTFTSVKSSVTGDDITATTTSGAI
                     AGSYDVVVNALAQSDRIQSQTAIPTATANGNSFGAGGSITVTLKNGNSETINLSGNST
                     TDLQGVVNAINNDGKAGV"
     gene            complement(658..1026)
                     /locus_tag="VGC69_13650"
     CDS             complement(658..1026)
                     /locus_tag="VGC69_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HEY0296369.1"
                     /translation="MDVSPILSTPASLPVQAVPVTPIEPIAPATGSAKGSGADSSTSQ
                     QQGGSNSSIDKALQSLNDEMQAWSTQLQFNFDPDLHRVVVSIVDSKTGKTINTIPSET
                     VLRIAKMITKFEGKAVQTEA"
     gene            1297..>2293
                     /gene="folC"
                     /locus_tag="VGC69_13655"
     CDS             1297..>2293
                     /gene="folC"
                     /locus_tag="VGC69_13655"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY0296370.1"
                     /translation="MSVPRPDAATPLDGWLQYLEGLHAKAIDLGLERVSEVARRLDIR
                     LDAVKFVVGGTNGKGSTCAMLESMLLAAGYKVGLYTSPHLIHFNERARLNGELVTDET
                     LVEQFQAVEAARGEISLTYFEFTTLAVLRLFSRSRLDAVVLEVGLGGRLDAVNIVDAD
                     CSIVTSVDLDHMDYLGDTREAIGFEKAHIYRAGRPAICSDPVPPQSLLDYAAKIGADL
                     WLFGHDFNYSGDRQQWAYGGRNQRRSALAYPALRGANQLLNASAAMAALESVRDRLSV
                     PQQAVRQGLALATLPGRFQILPGQPLVVLDVAHNPHAAAVLAQNLDNMGFHPYTYA"
BASE COUNT          372 a          673 c          779 g          469 t
ORIGIN      
        1 cacgccggcc ttgccgtcgt tgttgatggc gttgaccacg ccctgcaggt cggtggtgct
       61 gttgccgctg aggttgatgg tctcgctgtt gccgttcttc agcgtgacgg tgatggagcc
      121 gccggcgcca aagctgttgc cgttggccgt cgcggtgggg atggcggttt gcgactggat
      181 gcggtcggac tgggcgagcg cgttgaccac cacgtcgtac gagcccgcga tggcgcccga
      241 ggtggtcgtg gccgtgatgt cgtcgcccgt gaccgaggat ttcacggagg tgaaggtgct
      301 ggcctgaccc agcgtctggg cggcgctggc cacgccctgc acggcgctct gcagcgtgcc
      361 gtaggcggaa atctgggtct ggtagctgtt gatccggttc tggataacag tcaggctttg
      421 gttgtccgcg gcctggagcg aatccaggat gccttggaga tccaaactgg agccgacccc
      481 aagcgaggtg atcgtacccg cgttgctgct ggaggggatg gcgctagtgg tcatgtgatg
      541 ctccttcggc ctttgcaggc tagtacgtca attttgctta acaagcggcc ggcctgattg
      601 gctgggtaag agggagaaga cgcgtttgtt caaaagaaag gataagcgct cgtttcgtca
      661 ggcttcggtc tggaccgcct tgccttcgaa tttggtgatc attttggcga tgcgcagcac
      721 ggtttcggac gggatggtgt tgatggtctt gccggttttc gagtcgacga tgctgaccac
      781 gacgcggtgc aggtcggggt cgaaattgaa ttgcaattga gtggaccagg cctgcatctc
      841 gtcgttgagc gactggaggg ccttgtcgat ggacgagttc gagccgcctt gctgctgcga
      901 agtggaactg tccgcgccgc ttcccttggc cgagccggta gccggcgcga tgggttcgat
      961 gggcgtgact ggcacggctt gtacaggcag tgaagcaggg gtggaaagaa tcgggcttac
     1021 gtccatattt cactccggca agacatgcgc gaggcatggg ctggtggaat agtttctcgg
     1081 gtttcataac ggcatgcccc aagtaaactt tagaaatctg gcgggatgta cccgttatat
     1141 tgtcaataag cttaaaaaat tgacaagttt ggccggattg gccgggcacg caaatggggc
     1201 ggtggaatgc cggcgtggcc cgcccgcctg aaaaagtggg ggcggagtcg gtccgactgt
     1261 taaaatggcg ggctgtttca tcttcccctc tccctcatgt ctgtcccgcg ccccgatgcc
     1321 gccacccccc tggacggctg gctgcaatat ctggaaggcc tgcatgccaa ggccatcgac
     1381 ctggggctcg agcgggtaag cgaggtggcc cggcggctgg acatccggct cgatgcggtc
     1441 aaattcgtcg tgggcggcac caacggcaag ggctccacct gcgccatgct ggagtccatg
     1501 ctgctcgcgg cgggctacaa ggtggggctg tatacctcgc cgcatctcat ccacttcaat
     1561 gaacgggccc gcctcaatgg cgaattggtg acggacgaaa ccctggtcga gcaattccag
     1621 gccgtcgagg cggcgcgcgg cgaaatcagc ctgacctatt tcgaattcac caccctggcg
     1681 gtgctgcgcc tgttctcgcg cagccggctc gacgccgtgg tgctcgaagt ggggctgggc
     1741 gggcgcctgg acgcggtgaa catcgtcgat gccgactgct ccatcgtcac cagcgtcgac
     1801 ctcgaccaca tggattacct gggcgatacc cgcgaggcga tcggtttcga gaaggcgcat
     1861 atttaccgcg ccggccgtcc ggccatctgc agcgacccgg tgccgccgca atccctgctg
     1921 gattacgcgg cgaagatcgg cgccgacctg tggctgttcg ggcacgattt caattattcg
     1981 ggcgaccgcc agcagtgggc ctacggcggt cgcaaccagc gccgttccgc gctggcctat
     2041 cccgccttgc gcggcgccaa ccagctgctc aatgcctcgg ccgccatggc ggcgctggag
     2101 tcggtgcgcg accggctgtc ggtgccgcag caggccgtgc gccagggcct ggccctggcc
     2161 acgctgccgg ggcgcttcca gatcctgccc ggccagccgc tggtcgtcct ggacgtggcg
     2221 cacaacccgc atgcggcggc cgtcctggcg cagaacctcg acaatatggg cttccatccc
     2281 tacacctatg cgg
//
LOCUS       DASWPO010000261        23459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2829756, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000261 DASWPO010000000
VERSION     DASWPO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 23459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23459
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829756"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1782
                     /locus_tag="VGC69_13660"
     CDS             355..1782
                     /locus_tag="VGC69_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0296371.1"
                     /translation="MKLYEKLAGDIEDSIRRGVFHAGERIPSVRQSSQHHRLSITTVI
                     RAYLLLESRGLIESRPQSGYFVRFEALDEDGQPLRELRQSQPEPISAQVDMGQLVLST
                     LRAIGADEAVPLGSPYPDPALFPFEKINRYVYAIGRDKSKWGVTDGLPPGNQAFIRQI
                     ARRYLESGTAVDPNEIIVTVGATEAINLCLQAIGKPGDTVAVESPTFYAMLHAIERMG
                     MRAIEVPTHPQEGIDLGALSAILQRQPIAACMVMPNFQNPLGFQMPDEKKRELVELLT
                     RHDVPAIENDVYGELYFGAARPSTLKTYDRKGLVLHCASFSKSLTAAYRIGWAMPGRY
                     RAQVEKLKFLNTLTTPSIPQLAIAEYLEKDGYEHHMRRVRAMYQQQANLMRTVVDRFF
                     PQGTRVSHPRGGYVLWIELPPQVDSMRLYRLALEKRITVAPGYMFSTSNVYRHCIRLN
                     YSYAWSPEIERAVIEIGELAAACAR"
     gene            1943..2887
                     /locus_tag="VGC69_13665"
     CDS             1943..2887
                     /locus_tag="VGC69_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEY0296372.1"
                     /translation="MLQKTSAATLAENAPETRDAQYFEYTSSANPIGARLISRVPFRN
                     FAPSLYAQGATRIVPLDLSGELGCKGPATGPGLSASFLRINAGESMWLTPNATSQLCY
                     VIRGEGDLIQGEAAFPFAQGDFFTFPGGTPAELSARTDAALYYVNDAPLLAYLGTTGV
                     QPRFSPTLYPAARAQAELRKIAEDESAGKRNRISVLMGNSHFPQTRTVTHVLWAMFGI
                     VPPRSIQKPHRHQSIALDFIAGGGPGCYTLVGAELDAQGNIVNPTRVDWETGMAFVTP
                     PGYWHAHFNETDANAYVIPIQDAGLQTYLRTLDIRFAR"
     gene            complement(2851..3843)
                     /locus_tag="VGC69_13670"
     CDS             complement(2851..3843)
                     /locus_tag="VGC69_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296373.1"
                     /translation="MDYYAAVRAFVTAAALRSFSKTAQRLGVKTSTVSRHVSELEQDL
                     GIALFNRSTRGLVLTEGGQVFREYALQSLDALEDARQAASALNLTPQGQLRVTMPSAF
                     GRHHIAPHLPAFMARYPDIHVDLVVTDDTLNLIDARIDVAVRIGALPDSTLMARRLAS
                     HRRIACASPDYLRGHPAPAAPQDLASHAALRFARQPDDKWLFVRTGKGATGKSAAKPV
                     PVELRGSFRADDSEVILALALAGRGVALLPTWLAGPMLRDGRLAHLLPGWEARAEQAE
                     PAIWAVYPPKKIVSSKVRAFVDFYAETIGETPYWDDAPPPARAQRAKRMSKVRK"
     gene            4102..5334
                     /locus_tag="VGC69_13675"
     CDS             4102..5334
                     /locus_tag="VGC69_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296374.1"
                     /translation="MPAPSGTNWQRNLLVCLFGSFSTILGMTLMLPFLPIYVEQLGVH
                     GHAAIVQWSGVAYGAAFVTAGLVAPLWGHLGDRYGRKSMLIRASLGMAICVSLMGLCT
                     NIWQLVGLRLLIGLAGGYSSGSTILVAVQTPRERSGWALGLLSSGVMAGNLVGPLVGG
                     VLPQLIGLRATFWAAGALISITFLATVLLVKEAPRAAKKAVPKAAGAGTWSQIPRKQV
                     VIAMLATGLLLLFANMSIEPIITVYVSTLVSDPRQVTLVAGLAMSAAALGSILSASQL
                     GRLADRIGHSSVIVYALAVAALLLIPQAFVTAGWQLVVLRFLMGLALGGLLPCIAAVI
                     RHNVPEHGVGAMLGYSISAQFTGQFVGPVAGGFIGGHIGMRAVFLATSALLLLGSVYN
                     WCVVGRSGGKARVPVPHD"
     gene            complement(5355..5987)
                     /locus_tag="VGC69_13680"
     CDS             complement(5355..5987)
                     /locus_tag="VGC69_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0296375.1"
                     /translation="MQELPKPRKTPRQGRSQAMVEAILEATARVLAERGYAGTNTNVV
                     AERAGVSVGSVYQYFPNKDALIAALHERHAAQMHTAIDAVLVASRPDSLRDDIAAIVH
                     ALMAAHQAEPALHKVLEREFPFFDMPDEQSPADANIHGSLKSALETHRHEITPQDLDL
                     ATWLLMRLMESLVHAAIIDPPARFSSRDIEQGIIDVLMGYLLGAAKPPAP"
     gene            6080..6817
                     /locus_tag="VGC69_13685"
     CDS             6080..6817
                     /locus_tag="VGC69_13685"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEY0296376.1"
                     /translation="MFETRRRLPTIRAVGEVLSASAVTAHMQRIVLGGEALGPLLGME
                     GIDVPAAWVKVFTEAGDGRAYTVRALDRQAKTLTLDFVLHGGHGASGPVSAWAAGARA
                     GELVHLAGPRDGGYRCPADAGWMLLAGDATALPAIQSIARNLPDGVTADAYIEVPTPQ
                     DIQPDLADCRGVRVQWLPAGHAGSTLPAALASWRRPQGAGYFWAAGESSAMRHLSLQL
                     PALGIERQRADIKGYWKMGQADHRDRG"
     gene            complement(6831..7766)
                     /locus_tag="VGC69_13690"
     CDS             complement(6831..7766)
                     /locus_tag="VGC69_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296377.1"
                     /translation="MDLIECIGVFIEVGKSLSFSKAAETLGTSKSSITKKVVWLETYF
                     EVQLLNRNTKHVSLTESGRTLMEQADTLDFAVKGLKDQVQGPVRTPSGRIRVGTPPSF
                     GSVHLIPAINDFLRQYPAIRISLLLDDGRSDLIAENMDLSVRIAPRLKDTNQIAYRIT
                     VVPQVIVATRAYLDAHGMPASPKDLSRHNCLIHYLKAPTGIWNFTDSNGDSHAVTVSG
                     SMNSNLGEAILHSARLGYGISMHPRYMVDTYLESGALQILLPDYQPEGLDIYAVVQTK
                     RHLPYKVRLFVEHLRLWFKQADWTQPDSPAAPGRS"
     gene            7912..8781
                     /locus_tag="VGC69_13695"
     CDS             7912..8781
                     /locus_tag="VGC69_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0296378.1"
                     /translation="MEITESGATLGARIKGLDLGRPLSDAQRRAIEQALGRHGVVSFP
                     NQTLTARNLRDFAENFGTLEINVANLYHEPDLPEVMILSNLVENGKPIGLSDAGQDWH
                     TDMSYSETIAFSNVLYGLRIPVRGGQPLGNTEFCNMHAAYEELPRELKDELDGMTITH
                     DFNKFWEMMRREKGSTRAPLTEAQRRRKPPVSHPVFLTHPITGRKVLYANPGYSMRIN
                     ELSPARSEQVLAFLFEHQLQEKYRYRHRWTQGDVLMWDNMGTIHNAVADYRPDEPRLI
                     KRCQVMADRYFPQ"
     gene            8870..9838
                     /locus_tag="VGC69_13700"
     CDS             8870..9838
                     /locus_tag="VGC69_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296379.1"
                     /translation="MKTRILAGLALAAAGAFSSARADTYPSHPIYMVLGYEAGGPTDV
                     IARIVARYMSMSLHQSVIVENKPGASASIAASYVMRAPPDGYTMLVTSLTLNVNPLLY
                     PDRYNYDPIKSFEPVGNFANNPMVLVTHYNSPYKSVADLVKDAKATPDKLTFGSSGVG
                     GSAHLAAEMLANEAGIKMTHVPFKGNAPALQEAVAGRISFMFYPSVGIANLVAGKQLR
                     VLAVGSSKPRPDFPGVPTMDSLGYTGFEQGAPWVGMLAPAGTPRDIIDTLNKAAVEAL
                     DKPEVRQQLADLGAVVVADSPDQFRKFLIEDKARWEKVIRQGNVKP"
     gene            9853..11040
                     /locus_tag="VGC69_13705"
     CDS             9853..11040
                     /locus_tag="VGC69_13705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0296380.1"
                     /translation="MRIHLDDCYSIETLRAAARARLPRPIFDFYDGAAEDEMTLRENA
                     RAFQRVRLLPRVLRDVAEVDMRADLLGRPASLPLAIGPTGAVGFGWRGGDAALARSAA
                     RLGIPYALSTSATASIEDIATQAPGRHWFQAYILQDKDRLAKLIDRAAAADFEALVIT
                     VDLPVGGKRERDLAHGLALPMTLRARNVLQFARRPAWSLDMLLRRPPIMPSLAGMAPR
                     KADRKAMGSVAGTHYDPSFDVEALARLRDRWRGKLIVKGVVHPGDVDAILAVGADALV
                     VSNHGGRQLDTGVATLDALPGIVAAVAGRVPVWMDGGIRRGSDVFKALALGASAVLTG
                     RATLFGVLAGGEAGVDKALHILRDELARTMRLCGARGLGEITPDLLFDSRGTRFATMR
                     EKQ"
     gene            11037..11456
                     /locus_tag="VGC69_13710"
     CDS             11037..11456
                     /locus_tag="VGC69_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0296381.1"
                     /translation="MMSDTLMSCHNQVMGLFRDLDENRYESLAARFAPAGVWHRQGKI
                     LQGRQAIVQALNLRSRTQRIHHLIVNLVADRADSGRCALRGYMLVVRYDGGSPLAGPA
                     PLSGIENIRTTHIELSKSDGQWLVEQMRNDDPTFAAA"
     gene            11472..12233
                     /locus_tag="VGC69_13715"
     CDS             11472..12233
                     /locus_tag="VGC69_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0296382.1"
                     /translation="MKWIRYTLQDQVSYGLLDDERIIAVRGDPFSGHEKTGQVHRLGD
                     VRIEVPVVPPTFYCVGLNYVKHIATEGRQIPAKPDVGYRANNALVAHGQDVVMPADSS
                     RIHYEGELVVVIGKQAKHVSPGDAGSCILGYTIGNDVSERDWQASDRTFWRSKNSDTF
                     KPMGPWIDTEAHIEGMETIVRLNGKESTRFGTNDMLFGIDRFISEMSRYLTLYPGDVL
                     WMGTDGHSPDLQHGDVVDITLTGLGTLSNRFVREE"
     gene            12239..13231
                     /locus_tag="VGC69_13720"
     CDS             12239..13231
                     /locus_tag="VGC69_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEY0296383.1"
                     /translation="MQAAWYARNGKAQEVLELGELPTPEPGPGEVRVRLAASGVNPSD
                     VKSRAGSRPVPPGTLVVPHSDGAGIIDKVGDGVPAGRVGERVWIWNGQWQRPMGTCAQ
                     FIALPAGQAVPLPESVSFEAGACLGIPALTAIHAVERLGELAGKTVLVTGAASGVGYY
                     AAQMARLKGARVIGTVGSPDKAQALADAGIDALIHYKTEPVAERILSLTGGAGVDGIV
                     DLDFSTTARLVPAGAVARHGVFVCYGSNDRGATPIEFGSWLPRSISLAFFLVYDLLPA
                     ERRHALDALSALLAGGKLRHLLAPAYALADIVAAHQAVESGHVLGNVTVRLPAA"
     gene            complement(13286..13456)
                     /locus_tag="VGC69_13725"
     CDS             complement(13286..13456)
                     /locus_tag="VGC69_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HEY0296384.1"
                     /translation="MMTQNLLAIGAGLFVGLLFAGLKLPLPAPPTLTGILGAFGVFLG
                     SVLYRLIASLLA"
     gene            complement(13488..14036)
                     /locus_tag="VGC69_13730"
     CDS             complement(13488..14036)
                     /locus_tag="VGC69_13730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY0296385.1"
                     /translation="MASPLKIVGLAGSLRSASYSRSVLKSLAGLLPEGAQFASIDIGA
                     LPHYNEDAERADLPRSVREARELVSGCDAVFIVTPEFNHGLPGVLKNTLDWLSRPAFS
                     SCMVGRPVFFATLSPGALGGVRAQYQLRETLSSMLCRLVPLPEIAITGVGAKTAEGAL
                     TDAATLAFVGDQIKQFLAAIAR"
     gene            14150..14911
                     /locus_tag="VGC69_13735"
     CDS             14150..14911
                     /locus_tag="VGC69_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0296386.1"
                     /translation="MSSPSIEAVSTQHAAGVEIAPHAHGGGQLIIALRGTAIVMHDEG
                     AWLAPPGLGIWVPPNVVHSARYSESSDLINLQLGGSWAAQLSHACGSLVVSDLLRELA
                     RETVRLGALARPDPALDLLAQLIVLQIKAPKPGPMLFVPHGRDRRLQIATRALHENPG
                     QSLTLDGLAGLSHTSARTLARLFVAETGMTFGRWREHLRIVHAVDRLARGWSITQTAL
                     DLGYQSASSFTTLFTRLLGAPPRRYMQEFYVSKAR"
     gene            15230..15391
                     /locus_tag="VGC69_13740"
     CDS             15230..15391
                     /locus_tag="VGC69_13740"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY0296387.1"
                     /translation="MPPTYRGRQYVIDDWRGHHLHQPPRGYQWISTGADYFLIGIASG
                     AVLESVLGN"
     gene            complement(15442..15894)
                     /locus_tag="VGC69_13745"
     CDS             complement(15442..15894)
                     /locus_tag="VGC69_13745"
                     /inference="COORDINATES: protein motif:HMM:NF022885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2894 domain-containing protein"
                     /protein_id="HEY0296388.1"
                     /translation="MPNPMRSSPPPARQRPMRDGPSSGGPAVNRLPKRGLLAELTDHI
                     ARNAPLARPSYPELDMLEGFREIWSLVDMGRQLRQSQAQVPESAGPLNSSHLVHQALA
                     LMQAASPGYLQRFLSYLDTLSWLEQMHNNPGQNAARSKSSLPAGKQKP"
     gene            complement(15839..16522)
                     /locus_tag="VGC69_13750"
     CDS             complement(15839..16522)
                     /locus_tag="VGC69_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0296389.1"
                     /translation="MRDEIDTGPEAAATTWAAFGDLMSAMLGAFVLILLGIIGVQLEL
                     SSRLQQEVAQHQEETQRRVALEQALAGPLAAGRVTLVDGRIGINGSVLFLLNSDQLQP
                     EGREVLKSLAGPLSAYLRTRDEILMVSGFTDDQQVHEGNRRFADNWDLSAQRALTVTR
                     ALIDAGIPSSSIFAAAFGSEQPVTSNADEPGRAKNRRVEIAPIARQAPGHAQSDAQQP
                     ASGAATPHA"
     gene            complement(16519..18264)
                     /locus_tag="VGC69_13755"
     CDS             complement(16519..18264)
                     /locus_tag="VGC69_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296390.1"
                     /translation="MTRSLLHYVVFAAGLAVVAWVGVGYAGSNLLALAVTLLIGVAYL
                     AGALELQRYRRATGTLAQAVDSLSAAPGNLDGWLATLHASLRQAVRLRVEGVRAALPG
                     PGLAPYLAGLLVLLGMLGTFLGMIATLRGTGLALATGTDLQAIRDSLAAPVKGLGFAF
                     GTSVAGVAASAMLGLLSALCQRERALAAQGLDARIATTLRVFSPASQREETFKLLQRQ
                     ADALPALADRMQAMMAAMERQAQSLNDRLSATQEAFQHQAGAAYARLADTAAQALQAG
                     ASASARAAGEAIQPAVQATLAGLARDADAWRNAIAQSTREQVEGLADRFAAATTQVAD
                     RLDGHAQALAQRTSLLLDSLEQSHASRHRQLGETLARAADGIAAQAEARTQGTLAEMA
                     RLTQAAEQAPKAVADVIAELRRKQSEGIERDNAMLAERTRLLDTLGTLLDTVNQAAAR
                     QREAVDTLIDTAGERLGHMGERLTQSVEAETGKLAGMAGQMADGAAQAATLGAALGTA
                     LQSFSQSNDKLAEQLQRIEAALDKSMARSDEQLAYYVAQAREVVDLSLLSQKQILEDL
                     QRLPGQRDEARTAKA"
     gene            complement(18270..19016)
                     /locus_tag="VGC69_13760"
     CDS             complement(18270..19016)
                     /locus_tag="VGC69_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3348 domain-containing protein"
                     /protein_id="HEY0296391.1"
                     /translation="MVQVPKRPSLSGPALVRLLARLTDTPAVGPRTPLSGCLSQWLGW
                     SDAITLSTVLNNDPPAVPTGRAVPGDALRRECDRLRSGLLASIDGDGKPSPGHEPDRR
                     LRVQPALQQVAVDYAEYRQRYVSLQQSMESEIGHLRGRLRAMLAARSPEMTRLAMVDG
                     LMERALAARERNLLSLIPGMLEKHFERLRQAGAPDQADGPAQEPNLRWLAAFRLDMRS
                     VLRAELAVRMQSVEGLLAALLQPRQEAERN"
     gene            complement(19116..19847)
                     /locus_tag="VGC69_13765"
     CDS             complement(19116..19847)
                     /locus_tag="VGC69_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296392.1"
                     /translation="MRYLDFNSWQGLLSTVLGLLLITLMGVGMRLLIMQIIQQRQQRE
                     NRQINERLRTLMAVYKTLGGSFTGNLAVNPAHLKDGSRRDAAPSGAADNEADGAAPAR
                     PVSASDRARRMRDAAEAALSDIILLGTEQQVRLAVKAAADMVAGRPIHTDELVVSLRD
                     FIREALGLDPIPAGLEIPRQGPTRVQSGAAGARGKREADGAAGGGGRGGGGGGGMGAG
                     GGMGAGAGLGMGLGASHPLQDDHDE"
     gene            19850..20722
                     /locus_tag="VGC69_13770"
     CDS             19850..20722
                     /locus_tag="VGC69_13770"
                     /EC_number="3.11.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033978 - phosphonopyruvate hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate hydrolase"
                     /protein_id="HEY0296393.1"
                     /translation="MKKNRGKFDSTGFGAMDSAMAAHNPLSARLAEEAGFGGIWGSGF
                     ELSASYAVPDASILSLGTHLEMMRAIASTVSVPLIADIDTGFGNAVNVHYIVPQYEAA
                     GASAIVMEDKTFPKDTSLRAEGRQELVRIEEFQGKIAAAAAARKDSDFAVIARVEALI
                     AGLGQEEAIRRGLAYEEAGADAILIHSKQKTPDEILAFIDAWPGKAPLVLVPTAYPQL
                     AERDIAALGKVGVVIYGNHAIRAAVGAMRQVFAQIRRDGGIREVDRALPTVKEIIDLQ
                     GDAHMREVEGRYLR"
     gene            20954..21616
                     /locus_tag="VGC69_13775"
     CDS             20954..21616
                     /locus_tag="VGC69_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0296394.1"
                     /translation="MLTLFDHPRSGNCYKVRLFCSLAGIAYRSVFIDVLGRKNRSAEF
                     QAISPFCQVPAVRDGDDVVWDSQAILVYLAQRYARQWMPDPDAIAFSHMHQWLSVSSN
                     EIANSLQPLRLVYVVDPDEAAHHLGVSVEKFDLDGCIARSRRLLQCMERHLHGRTWLA
                     GNAPTIADIACHGYLSRAHETHIDMSDYPAVQAWNGHLQDLPGYLGMEASAHPASRNE
                     SA"
     gene            21687..22991
                     /locus_tag="VGC69_13780"
     CDS             21687..22991
                     /locus_tag="VGC69_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296395.1"
                     /translation="MSTIYSDVAVPAPPRRDSLLRIALATMVGSAIEGFDFLAYGTAA
                     ALVFNKLFFPNFAPELATLAAFAAFASGMLARPIGGILFGHFGDRLGRKSMLMLSLLL
                     MGVCTVLIGLLPPYTAIGIWAPVLLVVLRIGQGLSFGGEMGGAMLMAVEHAPPKWKSL
                     IGSMPQMAAPLGILISSVAFAIVTTLPESEFLLWGWRIPFVGSALLIVVGVFIRMKIS
                     ESPEFLAVKEDKRTAVVPAWNVIKQHWRPLILTITGKLGEVTLVFTILVFSISYAIGQ
                     LKFTRAEALNALLWGSLAMTFAIPLFGWVADKVGARRLYCLGGILLAIMAVPLFLAIG
                     SGSHSAYLVAIIIALALNYAIMFAPQSNLYAAQFPAELRYSGISMGIQFAAAIGGGLA
                     PIVATTLVGRYASIVPVGAYLAVLGLVSAISAYLMRPSSNER"
     gene            complement(23015..>23459)
                     /locus_tag="VGC69_13785"
     CDS             complement(23015..>23459)
                     /locus_tag="VGC69_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0296396.1"
                     /translation="ELPAMKAATAYFAEQALVSLLAAAYCESVSAAESSSDTETDRWL
                     RLVAFIDANLDDANLSVERIAHELKVSKRWVHRLFESHGQQYSAYLRQLRLQHAREEL
                     ESDRLRHLSVTEIGLRNGFRDSSHFSRSFREHYGIPPSSYRQASP"
BASE COUNT         3781 a         7678 c         7855 g         4145 t
ORIGIN      
        1 ggccaggcgc agcccgggca gtcgaagccg tcgggctggt tctgccggaa cagggtgcgg
       61 tagttgccgc cgggtacttt ttccttgacc agattgatgg ccacctgctt gagcgcgccc
      121 cagccggcgg cggggtggtg gtagggcttg atctgtcctt ggggtttgtc catctggtcg
      181 gaatccggcg gttgacggca cgtgtcgtgc ggcgagtccg gcaagattaa gcccatggtc
      241 ggcttcgggg agggtacaca tcctccatca ggaaaagagc acagttgttg aattgttgat
      301 taaaaaatgg aaaactgtac ctttaccgag ccgcaaccct ccgggagacg gagtctgaaa
      361 ctgtacgaaa agctggccgg cgacatcgag gactccatcc gccgcggcgt gttccacgcg
      421 ggcgagcgca ttccctcggt ccggcagtcc agccagcacc accgcctgag catcacgacc
      481 gtgatccggg cctacctgct gctggagagc cgggggctca tcgagagccg gccccagtcg
      541 ggctatttcg tgcgtttcga ggccctggac gaagacggcc agccgctgcg cgaattgcgc
      601 cagtcccagc ccgagcccat ctcggcgcag gtcgacatgg gccagctggt gctgtccacg
      661 ctgcgcgcca tcggcgccga cgaggccgtg ccgctgggct cgccgtaccc ggacccggcc
      721 ttatttcctt tcgagaagat caaccgctat gtctacgcca tcggccgcga caagagcaag
      781 tggggcgtga cggacgggct gccgcccggc aaccaggcct tcatccgcca gatcgcgcgg
      841 cgctacctcg agagcggcac ggcggtcgat cccaatgaaa tcatcgtgac cgtcggcgcg
      901 accgaggcca tcaatctatg cctgcaggcc atcggcaagc ccggcgacac ggtggcggtg
      961 gagtcgccca ccttctacgc catgctgcac gccatcgagc gcatgggcat gcgcgccatc
     1021 gaggtgccca cacatccgca ggaaggcatc gacctgggcg cgctgtcggc catcctccag
     1081 cgccagccca tcgccgcctg catggtgatg cccaacttcc agaacccgct gggcttccag
     1141 atgcccgacg agaaaaagcg cgaactggtc gaactgctga ctcgtcacga tgtgccggcg
     1201 atcgagaacg acgtctatgg cgagctgtat ttcggcgcgg cccgccccag caccttgaag
     1261 acctacgacc gcaaggggct ggtgctgcac tgcgcctcgt tctccaagag cctgacggcc
     1321 gcctaccgca tcggctgggc catgccgggc cgctaccgcg cgcaggtcga gaagctgaag
     1381 ttcctcaata cgctcaccac gccctccatt ccgcagctcg ccatcgccga atacctcgag
     1441 aaggacggct acgagcacca tatgcgccgg gtgcgcgcga tgtaccagca gcaggccaac
     1501 ttgatgcgca cggtggtgga ccgcttcttt ccgcagggca cgcgcgtgtc ccatccgcgc
     1561 ggcggctacg tgctgtggat cgaattgccg ccccaggtcg actccatgcg gctgtaccgg
     1621 ctggcgctcg agaagcgcat cacggtcgcg ccgggctata tgttctccac ctccaacgtc
     1681 taccggcatt gcatccgcct gaactacagc tacgcctggt cgccggagat cgagcgggcc
     1741 gtgatcgaga tcggcgagct ggcggcggcc tgcgcgcgct gaacgcgcga gcgcatcggc
     1801 cgcccgcttg tccccatccc gcaaaagtga tttccgcgcg cggcgcttcc ggcgcgcgcg
     1861 gcggcttcct atagtcgatg caacttccac gccggctttt tccctcggcc gcgggaagcc
     1921 ctcgaaccga caggagcgca tcatgctgca aaaaacctct gccgccacgc tggcggaaaa
     1981 cgcacccgaa acccgggacg cgcaatactt cgaatacacc tcttcggcca accccatcgg
     2041 cgccaggctg atttcccgcg tgccgtttcg caacttcgcg ccgtcgctgt acgcgcaagg
     2101 cgcaacgcgc atcgtgccgc tggatctttc cggggagctg ggttgcaaag ggcccgccac
     2161 gggaccgggc ctgagcgcca gcttcctgcg catcaacgcg ggcgagtcga tgtggctcac
     2221 cccgaacgcg acgtcccagc tgtgctatgt catccgcggc gagggcgatc tcatccaggg
     2281 cgaagcggcc tttccgttcg cgcagggcga tttcttcacg tttcccggcg gcacgccggc
     2341 cgagctgtcg gcgcggaccg acgccgccct gtactacgtc aacgacgcgc ccttgctggc
     2401 ctatctcggc accaccggcg tgcagccgcg cttctcgccc acgctgtacc ccgccgcgcg
     2461 ggcccaggcc gagctgcgca agatcgccga ggacgagagc gcgggcaagc gcaaccgcat
     2521 cagcgtgctc atgggcaaca gccacttccc gcagacccgc acggtgaccc acgtgctgtg
     2581 ggccatgttc ggcatcgtgc cgccccggtc gatacagaag ccgcaccgcc accaatcgat
     2641 cgcgctggac ttcatcgccg gcggcgggcc gggctgctat acgctggtcg gcgccgaact
     2701 ggacgcgcag ggcaatatcg tcaaccccac gcgggtcgat tgggaaaccg gcatggcctt
     2761 cgtcacgccg ccgggatact ggcacgcgca cttcaacgag accgacgcga acgcctacgt
     2821 gatccccatc caggacgcgg gcctgcagac ctacttgcgc accttggaca tccgcttcgc
     2881 gcgctgagcg cgggcgggcg gcggggcgtc gtcccagtac ggcgtctcgc cgatggtctc
     2941 ggcatagaag tcgacgaagg cgcgcacctt ggacgagacg attttcttcg gcggatagac
     3001 cgcccagatg gcgggctcgg cctgctcggc gcgggcttcc caaccgggca ggagatgcgc
     3061 cagccggccg tcgcgcagca tggggccggc cagccaggtc ggcagcaggg cgacgccgcg
     3121 gccggccagg gcaagcgcga ggatgacctc ggagtcgtcg gcgcggaagc tgccccgcag
     3181 ctcgaccggc accggcttgg ccgcgctttt gcctgtcgcg cccttgccgg tccggacgaa
     3241 cagccacttg tcgtcgggct gccgggcaaa gcgcagcgcc gcatgggacg ccaggtcctg
     3301 gggcgccgcg ggcgcgggat ggccgcgcag atagtccggg ctggcgcagg cgatgcggcg
     3361 gtgcgaagcc agccgtctgg ccatcagtgt cgagtcgggc agggcgccga tccgcacggc
     3421 cacgtcgatg cgcgcatcga tcaggttgag cgtgtcgtcg gtcaccacca ggtccacgtg
     3481 gatgtcgggg tagcgcgcca tgaaggccgg caggtgcggc gcgatgtggt gccggccgaa
     3541 tgccgacggc atggtcacgc gcaattggcc ctgcggcgtc aggttcaagg ccgaggcggc
     3601 ctgccgcgcg tcctcgagcg cgtcgagcga ctgcagcgca tactcgcgaa acacctgccc
     3661 gccctcggtc agcaccaggc cgcgcgtgga gcggttgaag agggcgatgc ccaggtcctg
     3721 ctccagctcg ctcacatggc gcgaaaccgt ggacgtcttg acgcccagcc gctgcgccgt
     3781 cttgctgaag ctgcgcagcg ccgccgccgt cacaaaggcc cgcactgcgg cgtagtagtc
     3841 catgcgtcga tgctcctttc cttcgcgcca tgccggttcg gccgggcggc tgcctactgt
     3901 agccgctgcc gcgcgcgaac gggcgctggt ctgaccagat ggcggccttg gtcgcggcgg
     3961 acggtatcat ttcccgcagc tgtccttccg cttcccgtat cggagtcgtg agtccgggct
     4021 ccccgctgcg cggttgagac gctgataaat ccgagattaa tgaataccaa attggcatac
     4081 caaaaaaaga gcaatattcc catgcccgct ccaagtggca cgaattggca acgcaatctg
     4141 cttgtctgtc tgttcggttc gttttcgacc atacttggga tgacgctgat gctgccgttc
     4201 ctgccgatct acgtcgagca gcttggcgtc cacggccacg cggcgatcgt gcagtggtca
     4261 ggcgtcgcct atggcgcggc gttcgtgacg gcggggctgg ttgcgccgtt gtggggacac
     4321 ctcggcgacc gctacgggcg caagtcgatg ctgatccgtg ccagcctcgg gatggccatc
     4381 tgcgtttccc tgatgggact gtgcaccaat atctggcagc tcgtcggctt gcggctgctg
     4441 atcgggctgg ccggcggcta ttcgtccggc tccacgatcc tggttgccgt gcagacgccc
     4501 agggaacgat ccggctgggc gctcggcctg ctgtcgtccg gcgtgatggc cggcaacctg
     4561 gtcggcccgc tggtaggcgg cgtgctgccg cagctgatcg gcctgcgggc gacgttctgg
     4621 gcggccggcg cgctgatctc catcaccttc ctcgcgacgg tcctgctggt gaaagaggcc
     4681 cctcgcgcgg caaagaaggc cgttcccaag gcagcgggcg caggaacctg gtcgcagata
     4741 ccgcgcaagc aggtggtcat tgcgatgctg gcgacgggct tgctcttgct gttcgccaat
     4801 atgtcgatcg agccgatcat caccgtctac gtcagcacgc tggtcagcga tcccaggcag
     4861 gtcaccttgg tggcgggcct cgccatgtcc gccgccgccc tgggcagcat tctgtcggct
     4921 tcccagctcg gccgcctggc cgacaggatc gggcattcat ccgtcatcgt ctatgcgctg
     4981 gccgtcgccg cgctgctgct gattccccag gcgttcgtga ccgccggctg gcagctcgtc
     5041 gtgctgcgct tcctgatggg gctcgccctg ggcggcctgc tgccttgcat cgcggccgtg
     5101 atccgccaca acgtgccgga gcatggtgtc ggggccatgc tcggctattc gatttccgcg
     5161 cagttcacgg ggcagttcgt cggccccgtg gccggcgggt tcatcggcgg ccatatcggc
     5221 atgcgggccg tgtttctcgc gaccagcgcc ttgctgctgc tcgggtctgt ctacaactgg
     5281 tgcgtggtcg ggcgctccgg cggcaaagcc cgtgtgcctg tgccccatga ctgatggggg
     5341 ttccatgcac ggcttcaagg cgccggcggc tttgcggcac cgagcaggta acccatcagc
     5401 acatcgatga tgccctgctc gatatcccgg gacgagaagc gcgcgggcgg atcgatgatg
     5461 gcggcgtgga cgagcgactc catgagccgc atcagcagcc aggtcgccag gtccagatcc
     5521 tggggcgtga tttcgtggcg gtgcgtttcc agggcgcttt tgaggctgcc gtgtatgttg
     5581 gcatccgccg ggctttgctc gtccggcatg tcgaaaaagg gaaactcccg ttcgagcacc
     5641 ttgtgcagcg ccggttcggc ttgatgggcc gccatgagcg cgtggacgat ggccgcgatg
     5701 tcgtcccgca ggctgtcggg gcgcgaggcc acgagcacgg cgtcgatggc cgtgtgcatc
     5761 tgggcggcat gccgttcgtg cagggcggcg atgagcgcgt ctttgttcgg aaagtactgg
     5821 tagaccgaac ccacgctgac cccggcccgt tcggccacga cattggtatt ggtgcccgcg
     5881 tagccgcgct cggccaaaac gcgagccgtc gcctcgagaa tggcttcgac catggcttgc
     5941 gagcgcccct ggcgcggcgt tttccgtggc ttgggcagtt cttgcatggc cgctccaaat
     6001 gcgagtttct aaatatgagt atttactcgt actatggtaa tccaatacga gtatttgctc
     6061 atatttttca ggagcggaca tgtttgaaac acgcaggcgg ctgccgacca tccgggcagt
     6121 cggggaagtg ctttccgcct cggcggtcac ggctcatatg cagcgcatcg tcctgggcgg
     6181 cgaagcgctc gggccgctgc tgggcatgga ggggatcgat gtgccggcgg catgggtcaa
     6241 ggtctttacc gaagcgggcg acgggcgcgc ctataccgtg cgggccctgg accgccaggc
     6301 gaagacgctg acgctggatt tcgtgctgca tggcggccac ggcgcgtccg ggccggtctc
     6361 ggcctgggcg gcgggcgccc gggccgggga actggtgcat ctcgccggtc ctcgcgacgg
     6421 cggctatcgc tgccccgccg acgcaggatg gatgctcctg gccggtgacg cgacggcctt
     6481 gccggcgatc cagtccatag cccggaacct gccggatggc gtgacggccg atgcctacat
     6541 cgaagtgccc acgccgcagg acatccagcc cgatctcgcc gattgccgcg gggtgcgggt
     6601 ccagtggctg cctgccggcc atgccggttc aacgctgccg gccgcgctgg cgagctggcg
     6661 gcggccgcaa ggcgcgggct atttctgggc ggcgggagaa tcgagcgcca tgcggcacct
     6721 gtccttgcaa ctgcccgccc tgggcatcga aaggcagcgg gccgacatca agggttattg
     6781 gaagatgggg caggcggacc accgagatcg cggctgaccg cgatccttct tcaagaccgg
     6841 cctggcgcgg ccgggctgtc gggctgcgtc cagtccgcct gcttgaacca tagccgcagg
     6901 tgttccacga acaggcgcac cttgtagggc aggtgccgct tggtctgcac cacggcatag
     6961 atgtccagcc cttcgggctg gtagtccggc agcaggatct ggagcgcgcc gctttccaga
     7021 tacgtatcca ccatatagcg tggatgcatg gagatgccat atcccaggcg ggccgaatgc
     7081 aggatggctt ctcccagatt ggaattcatc gaaccgctga cggtgacggc gtggctgtcg
     7141 ccgttgctgt cggtgaaatt ccagatgccg gtcggcgctt tcaaataatg aatcaggcaa
     7201 ttgtgccggc tcaggtcctt ggggctggcc ggcatgccgt gcgcatccag gtaggcccgc
     7261 gtcgcgacaa tgacttgcgg cacgaccgtg atgcgatagg cgatctggtt cgtgtccttc
     7321 agcctggggg cgatgcgcac ggaaaggtcc atgttttccg cgatcaggtc gctgcggccg
     7381 tcgtccagca gcagggaaat ccgaatggcc ggatattgcc gcaggaagtc gttgatggcc
     7441 ggtatcagat ggaccgatcc gaaggatggc ggcgtgccga cccggatgcg gccgctgggc
     7501 gtgcgtaccg ggccttgcac ctggtctttc aatcccttga cggcaaagtc cagggtgtcg
     7561 gcctgttcca tcaaggtccg gccgctttcc gtcaggctga cgtgcttggt attgcgattg
     7621 agcagttgca cttcgaagta ggtctccagc cagacgacct ttttggtaat ggacgatttg
     7681 ctggttccca gggtctccgc cgccttggaa aagctcaggc ttttacctac ctcgataaac
     7741 acgcctatac attcgatcaa atccatgtct gtttcttttt tggaaaaagt gatttttcat
     7801 gaatgggctt aaggcctggc ttcgagatta ctacactgcc gaattgcata aatagcacgg
     7861 cggcaatatt tgctttaact gtcgccttaa tagaaacagt aggagactgt catggaaatc
     7921 accgaatccg gcgcaacgct gggagccagg atcaagggcc tggacctggg ccggcctctg
     7981 tccgatgcgc agcgccgggc catcgagcag gcgctgggcc gccatggcgt ggtcagcttt
     8041 cccaatcaga cgctgacggc gcgcaacctg agggatttcg ccgaaaactt cggtacgctg
     8101 gaaatcaatg tggccaatct ttatcacgag cccgacctgc ccgaggtcat gattctttcc
     8161 aacctggtcg aaaacggcaa acccatcggc ttgagcgatg ccggccagga ctggcatacg
     8221 gatatgtcct acagcgagac gatcgccttc agcaacgtgc tgtatggact gcgcattccc
     8281 gtgcggggcg ggcagccgct gggcaacacc gagttctgca atatgcacgc ggcctatgag
     8341 gaattgccgc gggagttgaa agacgaactc gacggcatga ccatcaccca cgatttcaac
     8401 aaattctggg aaatgatgcg gcgcgagaaa ggcagcacgc gtgcgccgct caccgaggcg
     8461 cagcgccgca ggaagccgcc cgtttcccat ccggtattcc tgacgcatcc catcaccggc
     8521 cggaaggtcc tgtatgccaa cccgggctat tccatgcgca tcaacgagtt gtccccggcc
     8581 cgcagcgagc aggtgctggc cttcctgttc gaacaccagc tccaggaaaa ataccgctat
     8641 cgccatcgct ggacgcaagg cgatgtattg atgtgggaca acatgggcac catccacaat
     8701 gccgtggccg actaccggcc cgacgaaccg cgcctgatca agcgctgcca ggtgatggcc
     8761 gatcgctatt tcccgcagta agcctcgcct tgcgaggcgc attgttcacg gctgcgcaag
     8821 gcgctggaaa gcgccggcgg ccgaaaaatc aaaaaaacgg agacaaacca tgaaaaccag
     8881 aatcctggcc gggctcgcgc tggccgcggc cggcgctttt tccagcgcgc gggccgacac
     8941 gtatccctct catcccattt acatggtcct tggctacgag gcgggcggcc ctaccgatgt
     9001 cattgcccgc atcgttgcca gatacatgtc catgagcctg catcaatcgg tcatcgtcga
     9061 aaacaagccg ggagccagtg cttccatcgc ggccagctac gtcatgcgtg cgccgccgga
     9121 cggctacacc atgctggtca cgtccctgac gctgaacgtc aacccgctgc tgtatcccga
     9181 tcggtacaac tacgatccca tcaagtcgtt cgaacctgtc ggcaacttcg ccaacaaccc
     9241 gatggtgctg gtcacccact acaactcccc ctacaagtcg gtggccgacc tggtcaagga
     9301 tgccaaggcc acgcccgaca agctgacttt cggctcttcg ggcgtcggcg gctcggccca
     9361 cctggccgcc gagatgctgg ccaacgaggc cggcatcaag atgacgcacg tccctttcaa
     9421 gggcaatgcg ccggctttgc aagaggccgt ggcgggccgc atttcgttca tgttctatcc
     9481 cagcgtgggt atcgccaacc tggtcgccgg caagcaactg cgggtgctgg cggtaggcag
     9541 cagcaagccg cgcccggatt ttccaggggt tcccacgatg gatagcctgg gctatacggg
     9601 tttcgagcag ggggctccct gggtgggcat gctggccccg gcgggaacgc ctcgagacat
     9661 tatcgatacc ctgaacaagg cggccgtcga ggcgctcgac aagccggagg tgcgccagca
     9721 attggccgac ctgggcgccg tggtcgtggc cgactcgccg gatcaattcc ggaagttcct
     9781 catcgaggac aaggcccgct gggagaaggt gatccgccaa ggcaatgtga agccctgaac
     9841 ccggttgcca cgatgcggat ccatctcgac gactgctatt cgatagaaac cttgcgcgcg
     9901 gcggcccggg cccgtttgcc gcgtcccatc ttcgatttct acgacggcgc cgccgaggac
     9961 gaaatgacct tgcgcgagaa cgcccgcgct ttccagcgcg tgcgattgct gccccgggtg
    10021 ctgcgcgacg tcgccgaggt ggatatgcgg gccgatctgc tcggccgccc ggcctccttg
    10081 ccattggcga tcgggcccac cggcgcggta ggtttcggct ggcgcggggg cgacgcggcc
    10141 ctggcgcgca gcgcggcccg gcttggcatt ccttatgcct tgtcgacttc cgccacggca
    10201 tccatcgagg acatcgcaac gcaggcgccg ggccggcact ggttccaggc ctatatcctg
    10261 caggacaagg acaggctcgc caagctgatc gaccgggccg ccgccgcgga tttcgaggcc
    10321 ttggtcatca ccgtggactt gccggtgggc ggcaagcggg agcgcgacct ggcgcatgga
    10381 ctggctttgc ccatgacgct gcgggcaagg aatgtgctgc aattcgcgcg caggccggca
    10441 tggtccctgg acatgctgct gcgccgtccg cccatcatgc ccagcctggc cggcatggcg
    10501 ccgcgcaagg ccgaccgcaa ggccatggga tcggtggccg gcacgcacta cgacccgtct
    10561 ttcgatgtcg aggcattggc gcgcctgcgc gaccgctggc gcggcaagct catcgtcaag
    10621 ggcgtggtgc atccgggcga cgtggatgcc attcttgccg tgggcgccga tgccttggtt
    10681 gtctccaacc acggcggccg gcagctcgac acgggcgtgg cgacgctgga cgccttgccc
    10741 ggcatcgtgg cggccgtggc gggccgggtg ccggtatgga tggacggcgg catccggcgc
    10801 ggcagcgatg ttttcaaggc gctggcattg ggcgcatccg cggtgctgac gggcagggcg
    10861 acgcttttcg gcgtgctggc cggcggcgag gcgggtgtcg acaaggcgct gcatattctt
    10921 cgcgacgaac tggcgcgcac catgcgcctg tgcggcgccc gcggcctggg cgaaatcacg
    10981 cccgaccttc ttttcgattc gcgcggtacc cgtttcgcga ccatgaggga gaaacaatga
    11041 tgagcgacac cttgatgtca tgccataacc aggtcatggg gctgttccgg gatctggatg
    11101 aaaaccgtta cgaatcgctg gcggcgcgct ttgcccccgc gggcgtctgg caccggcagg
    11161 gcaagatcct gcagggacgc caggccatcg tgcaggcctt gaatctgcga tccaggaccc
    11221 agcgcatcca tcacttgatt gtcaacctgg tcgccgaccg ggccgattca ggccgctgcg
    11281 cgctgcgcgg gtatatgctg gtcgtgcgat acgacggcgg ctcgcctttg gccggcccgg
    11341 cgcccttgtc cggcatcgag aatatccgga ctacccatat cgaattgtcc aagtccgatg
    11401 ggcaatggct ggtcgagcaa atgcgcaacg acgaccccac ctttgccgcg gcctgacagg
    11461 agacacccgc catgaaatgg atacgctata cccttcaaga tcaggtgtcg tacgggctgc
    11521 tcgacgacga gcggatcatc gccgtgcgcg gcgacccgtt ctccggccat gagaaaaccg
    11581 gccaggtcca tcgcctgggc gatgtcagga tcgaagtgcc ggtggttccg ccgacgttct
    11641 attgcgtcgg cctgaattac gtcaagcata tcgcgacgga aggccggcaa attcccgcca
    11701 agcccgacgt cggataccgc gccaacaacg cattggtcgc gcatgggcag gacgtggtca
    11761 tgcccgccga ttcctcccgc atccattacg agggcgagct cgtggtggtg atcggcaagc
    11821 aggccaagca cgtcagcccc ggggacgccg gatcctgcat attgggctac accatcggaa
    11881 atgacgtcag cgagcgcgac tggcaggcgt cggatcgcac attctggcgt tccaagaaca
    11941 gtgatacgtt caagcccatg ggcccctgga tcgataccga ggctcatatc gagggcatgg
    12001 aaacgatagt ccggctcaac ggcaaggaaa gcacccgctt cggcaccaac gacatgctgt
    12061 tcggcatcga ccgcttcatc agcgagatga gccgctacct gacgctgtat cccggcgacg
    12121 tgctctggat gggaaccgac gggcattcgc ccgatctgca gcatggcgac gtggtggaca
    12181 tcacgctgac cgggctggga accttgagca accgcttcgt acgcgaggag tgatcgtcat
    12241 gcaggcagcc tggtatgcac gcaacggcaa ggcgcaggag gtgctggaac tgggcgagct
    12301 gcccacgccc gaacccggcc ccggcgaggt ccgggtcagg ctggccgcct ccggcgtcaa
    12361 cccctcggac gtcaaatccc gggcaggcag ccggccggtc ccgccgggca ccctcgtcgt
    12421 gccccatagc gatggcgccg gcatcatcga caaagtcggc gacggcgtgc cggcgggcag
    12481 ggtgggcgaa cgcgtctgga tatggaacgg ccagtggcag cgtccgatgg gtacctgcgc
    12541 ccagttcatc gcgctgccgg ccgggcaagc cgtgccgctg cccgagtccg tttccttcga
    12601 agccggcgcc tgcctgggca tccccgcgct cacggccatc catgccgttg agcggctcgg
    12661 cgagcttgcc ggcaagaccg tcctggtgac gggggcggcg tccggcgtcg gttactacgc
    12721 ggcgcagatg gcgcggctca agggcgcgcg cgtgattggc acggtgggct cgccggacaa
    12781 ggcgcaagcc ctggccgatg cgggcatcga tgcgctgatc cactacaaga ccgaaccggt
    12841 ggccgagcgc atcctgtcct tgaccggcgg ggctggcgtc gacggcatcg tcgacctgga
    12901 cttctcgacc acggcgcgcc tggttcctgc gggggccgtt gcgcggcacg gcgttttcgt
    12961 ttgctatggg tcgaacgacc gcggcgcgac gccgatcgaa ttcggcagtt ggctgccgcg
    13021 ctcgatcagc ctggcgttct tcctggtcta cgacctgctg cccgctgaac gccggcatgc
    13081 cctcgatgcc ctgagcgcgc tgctggccgg cggcaagctc cgccacttgc ttgccccggc
    13141 ctatgcgctg gccgatatcg tggcggcgca ccaggccgtg gaaagcgggc atgtgctggg
    13201 caacgtcacc gtgaggctgc cggcggctta gcccccggtc cttgcgtgat ggacccgggc
    13261 cgcgccgcgc ccggccttgc cccgctcaag ccagcagcga agcgatgagc cggtacagca
    13321 cgctgcccag gaacacgccg aacgcgccca ggatcccggt cagcgtcggg ggcgcgggca
    13381 acggcagctt gagccctgcg aacaaaaggc cgacaaacag gccggcgccg atggctagga
    13441 ggttttgcgt catcatgcct gggtcgttcc tggtcgagga tcgtgcttca gcgcgcgatg
    13501 gctgcaagga attgcttgat ctggtcgccg acgaaggcca gcgtcgccgc gtcggtcaag
    13561 gcgccttccg ccgttttcgc gccgactccc gtgatcgcga tttccggcag cggcaccagg
    13621 cggcacagca tcgacgagag cgtttcgcgc agttggtact gggcgcgtac gccgcccagg
    13681 gcgccgggcg acagcgtggc gaaaaatacc ggcctgccca ccatgcagct gctgaaggcc
    13741 gggcgggaca gccagtcgag cgtgttcttg agcacgccgg gcaggccatg gttgaactcg
    13801 ggcgtcacga tgaacacggc atcgcagccg gacaccagct cgcgcgcctc gcgcacggag
    13861 cggggcaggt ccgcgcgttc ggcgtcttcg ttgtagtggg gcagcgcgcc gatgtcgatc
    13921 gatgcgaatt gcgcgccctc cggcaacaag ccggccaggc tcttgaggac gctgcgcgaa
    13981 taagaggcgg aacgcaggct gccggcaaga ccgacgattt tcagggggga ggccatacga
    14041 atactccagg aaagcggcgc cgccatgggc accgggacgg ttgtattatt tgataaataa
    14101 aagccgattt ctttcgctat cctgccatcc tctgccgctg ggctgccata tgtcttcccc
    14161 atccatcgaa gccgtctcga cacagcacgc ggcaggcgtg gagatcgctc cccacgcgca
    14221 tggcgggggc cagctgatca tcgcgctgcg gggtacggcg atcgtcatgc acgacgaagg
    14281 cgcatggctc gcgccgccgg ggctcggcat ctgggtgccg cccaatgtcg tccatagcgc
    14341 ccgctatagc gagtcctccg acctgatcaa tctgcagctg ggcggttcct gggcggcgca
    14401 gctttcccat gcctgcggct cgctcgtcgt gtccgacttg ctgcgcgaac tggcgcgcga
    14461 aaccgtccgg ctcggcgcgc tggccaggcc ggatcccgcg ctcgatctgc tggcgcaatt
    14521 gatcgtgctg cagataaagg cccccaagcc ggggccgatg ctgttcgtgc cgcatggccg
    14581 ggaccggcgt ttgcagatcg ccacccgcgc cctgcacgag aatccgggcc aatccctgac
    14641 cctggacggg cttgccgggc tgtcccacac cagcgcgcgc acgcttgccc gcctgttcgt
    14701 cgccgagacc ggcatgacct ttggccgctg gcgcgagcac ctgcgcatcg ttcatgccgt
    14761 ggatcggctc gcgcgcggct ggtccattac ccagacggcg ctggatctcg gctaccagag
    14821 cgcgagcagc tttacgacgc tgttcacgcg cctgcttggc gcgccgccgc ggcggtatat
    14881 gcaggagttt tatgtttcga aagcccgata ggccactgtt tattaataat ggttgcggat
    14941 ggcagcagac ttgtcacgcg gtcttgatag aatctgccca tacggcgcat tgccgggttg
    15001 ctggagaaag gccatgaaca agaagaacgt cgtctctacc ctgttggcgg cggtgttggc
    15061 ggtcagcgcc agcgccgcgt tggcacagcc tcgtccaggc ccggaccacc ggggcggtcc
    15121 gagccatggc gctccgggcc atggtgcgcc gggccaccgt ccggagccgg gccgcggccc
    15181 cgatttcagg cctgatccgc cgcctgccca ttgggccagg ggcgaccacg tgccgccgac
    15241 gtatcgcggc cggcaatacg tcatcgacga ttggcgtggc caccatctgc accagccgcc
    15301 gcgcggatac cagtggatct ccacgggcgc ggattacttc ctgatcggca ttgcctcggg
    15361 cgccgtgctg gagtcggtgc tgggcaactg aagaaccggg tttcgccgcc cgcagcgagc
    15421 cgacccgagg ggtcggcttt tttacggctt ttgcttgccc gccggaaggc tgctttttga
    15481 tcgggccgcg ttctggcccg gattgttgtg catctgctcc agccacgaca gcgtatccag
    15541 gtaggacaga aagcgttgca gatagccagg cgaagccgcc tgcatcagcg ccagggcctg
    15601 gtgcacgaga tggctggagt tgagcggccc ggcgctttcg ggcacttgcg cctgcgactg
    15661 ccgcaactgc cggcccatgt cgacgagcga ccatatttcg cgaaagccct cgagcatgtc
    15721 cagctcggga tagctgggac gcgcgagcgg cgcgttgcgg gcgatatggt ccgtcagctc
    15781 ggccaacagg ccgcgcttgg ggaggcgatt cacggcgggg ccgccagaag acgggccgtc
    15841 acgcatgggg cgttgccgcg ccggaggcgg gctgctgcgc atcggattgg gcatggcccg
    15901 gggcctggcg cgcgatgggc gcgatctcca cgcgccggtt ctttgcccgg cctggctcgt
    15961 ccgcgttcga tgttaccggt tgttccgagc caaaggcggc cgcgaagatc gaggacgagg
    16021 ggatgccggc gtcgatcagc gcgcgggtca cggtcagcgc gcgctgggcc gagaggtccc
    16081 agttgtccgc aaaacgccgg ttgccttcgt gcacctgctg gtcgtcggtg aagccgctga
    16141 ccatgaggat ttcatcgcgg gtgcgcaaat aggccgacag ggggccggcc aggctcttga
    16201 gcacttcgcg gccctcgggc tgcaactggt cggagttgag caggaagagc acgctgccgt
    16261 tgatgccgat gcgcccgtcg accagggtga cgcggccagc cgcgagcggc ccggccagcg
    16321 cctgctccag ggcgacccgg cgttgggttt cttcctggtg ctgcgcgact tcttgctgca
    16381 ggcgggatga cagctccagc tgcacgccga tgatgcccag caggatcagc acaaaggcgc
    16441 ccagcatggc cgacatcagg tcgccaaagg cggcccaggt cgtggcggcc gcttccgggc
    16501 cggtgtcgat ctcgtctctc atgccttggc cgtgcgcgct tcatcccgct ggccaggcag
    16561 gcgctgcagg tcttccagga tctgtttctg cgacagcagg ctgaggtcga ccacctcgcg
    16621 ggcctgggcc acgtagtagg ccagctgctc gtcgctgcgg gccatggatt tgtcgagcgc
    16681 ggcctcgata cgctgcagct gttcggccag cttgtcgttg gactggctga acgattgcaa
    16741 ggccgtgccc agggccgcgc ccagggtggc ggcctgggcc gcgccgtccg ccatctgccc
    16801 ggccatgccg gcgagcttgc cggtctcggc ctcgacggac tgggtgagtc gttcgcccat
    16861 gtgtccgagc cgctcgccgg cggtgtcgat cagcgtatca acggcctcgc gttggcgggc
    16921 cgcggcctgg ttcacggtgt cgagcagcgt gcccagcgtg tccagcaggc gggtgcgttc
    16981 cgccagcatg gcattgtcgc gttcgatgcc ttccgactgc ttgcgccgca attcggcaat
    17041 gacatcggcc acggccttgg gggcttgctc ggcggcttgc gtgaggcggg ccatctcggc
    17101 cagcgtcccc tgggtgcgcg cctcggcctg ggcggcgatg ccgtcggcgg cgcgggccag
    17161 cgtttcacct agctgtcgat gccggctggc gtgggattgc tccagcgagt ccagcagcag
    17221 gctggtgcgt tgcgccaggg cctgggcatg gccgtcgagc cggtcggcga cctgggtggt
    17281 cgcagccgcg aaacggtcgg ccaggccttc gacctgttcg cgcgtggatt gcgcaatggc
    17341 gttgcgccag gcatcggcat cgcgcgccag gccggccagc gtggcctgca cggcaggctg
    17401 gatggcttcg cccgccgcgc gggcgcttgc cgaggccccg gcctgcaggg cttgcgcggc
    17461 cgtgtcggcc aggcgcgcgt aggcagcgcc cgcctggtgc tggaaggcct cctgggtggc
    17521 ggacaggcga tcgttcaagg actgggcctg gcgttccatc gccgccatca tggcttgcat
    17581 gcgatcggcc aaggcgggca gggcatccgc ctgccgctgc agcagcttga acgtttcctc
    17641 gcgctggctg gccggtgaaa acacgcgcaa ggtcgtggcg atgcgcgcgt ccagcccctg
    17701 cgccgccaag gcgcgctcgc gctggcacag cgccgacagc agccccagca tggccgaagc
    17761 ggccaccccc gccaccgagg tgccaaaggc aaagcccagg cctttcaccg gcgccgccag
    17821 ggagtcgcgg atggcctgca ggtcggtgcc tgtcgccagc gccaggccgg tgccgcgcag
    17881 ggtcgcgatc atgcccagga aggtgcccag catgcccagc agcaccagca ggcccgccag
    17941 gtagggcgcg agtcccggcc ccggcaaggc cgcccgcacg ccctcgacgc gcaggcgcac
    18001 ggcctggcgc aggctggcat gcagggtggc gagccagccg tccaggttgc ctggcgcggc
    18061 ggacaggctg tccacggctt gcgccaaggt cccggtcgcg cggcgatagc gctgcagctc
    18121 aagcgcgccg gccaggtagg ccacgccgat cagcagcgta acggccagcg ccagcaggtt
    18181 ggatccggca tagcccacgc ccacccaggc caccacggcc agcccggccg caaacaccac
    18241 ataatgcaaa agagatcggg tcatcgcgtt caattccgtt cggcttcttg tcggggttgg
    18301 agcagggcgg ccagcagtcc ttcaacggac tgcatccgca ccgccagctc ggcccggagc
    18361 acgctgcgca tgtccagccg gaaagcggcc agccacctga ggttgggctc ctgggccggg
    18421 ccgtcggctt ggtccggcgc gccggcttgc cgcaggcgct cgaaatgctt ctcgagcatg
    18481 ccgggtatca gcgacagcag gttgcgctcg cgcgcggcca gtgcgcgctc catcaggcca
    18541 tcgaccatgg ccaggcgcgt catctccgga gagcgcgccg ccagcatggc gcgcaggcgg
    18601 ccacgcaggt ggccgatctc cgactccatg ctttgctgca gcgagacata gcgctggcga
    18661 tattccgcat agtcgacagc cacctgttgg agcgccggct gcacgcgcaa gcgccggtcg
    18721 ggctcatggc cgggcgaggg cttgccgtcg ccgtcgatgc ttgccagcaa gccggatcgc
    18781 agccggtcgc actcgcgccg cagcgcgtcg cccgggacgg ccctgccggt gggcacggcc
    18841 ggcggatcgt tgttcaggac cgtggacagc gtgatggcgt cggaccagcc cagccattgg
    18901 ctcaggcagc cggagagcgg cgtccggggg ccgacagcgg gcgtatcggt caggcgcgcg
    18961 agcaagcgga cgagcgccgg gccgctgagg ctggggcgct taggcacttg caccatatgt
    19021 tcagggttaa aaagctctcg attttacatg ccgcagggag atggggcacg gccgctttgt
    19081 tccagaactt atcagccgga gaggggccgg tttttttact cgtcgtgatc gtcctgcaaa
    19141 gggtgcgagg cgcccagtcc catgcccagg ccggcccccg cgcccatgcc gccccctgcg
    19201 cccatgccgc cgccaccacc gccgccgcgt ccgccgccgc cagccgcgcc atcggcttcg
    19261 cgcttgccgc gcgcgccggc ggcgcccgac tgcacgcggg tgggtccctg cctgggaatc
    19321 tccagcccgg cggggatcgg atccaggccc aaggcctcgc ggatgaagtc gcgcagcgac
    19381 accacgagtt cgtcggtatg gatgggacgg ccggccacca tgtcggcggc ggccttcacg
    19441 gccaggcgca cctgctgctc ggtgcccagc aggatgatgt cggacagggc ggcttccgcc
    19501 gcgtcgcgca tgcggcgggc ccggtcgctg gcggaaacgg gccgggccgg ggcggcgccg
    19561 tccgcctcgt tgtcggctgc cccggacggc gcggcatcgc gccgcgaccc gtctttcagg
    19621 tgggcggggt tgaccgccag attgccggtg aacgagccgc ccagcgtctt gtacaccgcc
    19681 atcagcgtgc gcaggcgttc gttgatctgc cggttttcgc gttgctggcg ctgctggatg
    19741 atctgcatga tgagcagccg catgcccacg cccatcaggg tgatgagcag cagccccagg
    19801 acggtcgaga gcagcccttg ccaggagttg aagtccagat agcgcatgga taaagaaaaa
    19861 ccgtggaaag ttcgattcta cggggtttgg tgcaatggat tcggccatgg ccgcgcacaa
    19921 cccgctgtcg gccaggctgg ccgaagaggc ggggttcggc ggcatctggg gcagcggttt
    19981 cgagctctcg gccagctatg ccgtccccga cgccagcatc ctttccctgg gcacccatct
    20041 ggagatgatg cgcgccatcg cctccacggt ttccgtgcct ttgatcgccg atatcgatac
    20101 cggcttcggc aatgccgtga acgtgcacta catcgtgccg caatacgaag cggcgggggc
    20161 ttcggccatc gtgatggaag acaaaacctt ccccaaggac acgagcctgc gcgccgaggg
    20221 ccggcaggaa ctggtgcgca tcgaggagtt ccagggaaag atcgccgccg cggcggcggc
    20281 gcgcaaggac agcgacttcg ccgtgatcgc ccgggtcgag gccttgatcg ccggcctggg
    20341 ccaggaagaa gccatccggc gcggcctggc ctatgaagaa gccggcgcgg atgccatcct
    20401 catccactcc aagcaaaaga cgcctgatga gatcctggcc ttcatcgatg cctggccggg
    20461 caaggcgccg ctggtgctcg tgcccaccgc ctatccccag cttgccgagc gcgacatcgc
    20521 ggcgctgggc aaagtgggcg tcgtcatcta cggcaaccac gccatccgcg cggccgtggg
    20581 ggccatgcgc caagtgttcg cgcagatccg ccgggacggc ggcatccgcg aagtcgaccg
    20641 ggcgttgcct acggtcaagg agatcatcga tctgcagggc gatgcgcaca tgcgcgaagt
    20701 cgaggggcgc tatctccgat aggccatgac ggcgagttgt ctccacgcgg aattcctcgt
    20761 gtaaacccgg agaattccgc gtaaacccga gtgcaggcgc gaggctttcc cgtatgccgg
    20821 cgcgctggat caattgcagt gcgcttagga gcaagcgctc cggccggcac actccttagc
    20881 atgacgcatc gatccgacag gccgccgcgg cggtggccag ggcggtcatg ccgatcatga
    20941 gggagagcct gccgtgttga cgctgttcga ccacccgcgc tcgggtaatt gctacaaggt
    21001 ccgcctgttc tgttcgctgg cgggcattgc gtatcgatcc gttttcatcg atgtcctggg
    21061 ccgcaagaac cgcagcgcgg aattccaggc gatcagcccc ttctgccagg ttccggctgt
    21121 gcgggacggc gacgatgtcg tctgggacag ccaggccatt ctggtgtacc tggcccagcg
    21181 atatgcccgc cagtggatgc cggatccgga cgccattgcg ttctcccata tgcatcagtg
    21241 gctgtcggtt tcgtcgaacg agatcgcgaa cagtctgcag cctttgcgcc tggtgtacgt
    21301 cgtcgatccc gacgaggcgg cccaccacct gggcgtttcg gtcgagaagt tcgacctgga
    21361 tggctgtatc gcgcgatccc gccgcctgct gcaatgcatg gagcggcatt tgcatggccg
    21421 cacctggctg gcgggcaatg cgccgacgat tgccgacatc gcctgccacg gctacctgtc
    21481 ccgtgcgcac gagacgcata tcgatatgtc cgactatccc gccgtgcagg cgtggaatgg
    21541 gcatctgcag gacttgcccg gctatctcgg catggaggcg tcggcccatc cggcgtcccg
    21601 caacgaatcg gcgtaggcgc cgtagaggcc acgacgccgc cagctcgtcg tgcgcatcgc
    21661 ccatatccat tatcaagggg aaaaccatga gcactattta ttcggacgtc gctgtcccgg
    21721 cgcctcctcg ccgggacagc ctgctgcgca ttgcgctggc caccatggtc gggtccgcca
    21781 tcgaaggctt cgacttcctg gcttacggca ccgcggcggc attggtcttc aataaactct
    21841 tttttccaaa cttcgcgccc gagctggcga cgctggcggc ctttgccgca tttgccagcg
    21901 gcatgctggc ccgccccatc ggcggcattt tgttcgggca tttcggcgat cgcctgggca
    21961 ggaagtccat gctgatgctg agcctgctgc tgatgggcgt ctgcaccgtg ctgatcggcc
    22021 tcctgccgcc ctataccgct atcggcatat gggcgccggt gctgctggtc gtcctgcgta
    22081 tcgggcaggg cttgtcgttc ggcggagaga tggggggcgc catgttgatg gcggtcgaac
    22141 atgcgccgcc caagtggaaa tcgctgatcg gcagcatgcc gcagatggcg gcgcccctgg
    22201 gcatactgat ctcgtcagtg gcattcgcca tcgtcacgac cctgcccgaa tccgagtttc
    22261 tgctttgggg gtggcgcatc cctttcgtag gcagtgcgct cttgatcgtg gtcggcgtat
    22321 tcatccgcat gaaaataagc gagtccccgg agttcctggc cgtcaaggag gacaagcgca
    22381 ctgccgtggt gccggcatgg aacgtgatca agcagcattg gcggccgctg atcctcacga
    22441 tcaccggcaa gctcggcgaa gtgacgctgg ttttcacgat cctggtcttt tcgatttcct
    22501 atgccatcgg tcaattgaag ttcacgcggg cggaggcctt gaacgcattg ctttggggct
    22561 cgctggccat gacgttcgcc atacccctgt tcggctgggt cgcggacaag gtgggagccc
    22621 gccggctgta ctgcctgggc ggcatcctgc tggcgatcat ggcggtgccg ctgttcctgg
    22681 ccataggcag cgggtcccat tccgcctatc tcgttgcgat catcatcgcg ttggcattga
    22741 actacgccat catgttcgcg ccccagtcga acttatacgc cgcgcagttt cccgcggagc
    22801 tgcgctattc cggcatatcg atgggcatcc agttcgcggc ggccatcggc ggcggcctgg
    22861 cgcccatcgt cgcgacgacg ctggtggggc ggtatgcgag catcgtgccc gttggggctt
    22921 atctcgcggt gctgggcttg gtctcggcca tctcggccta tttgatgcgg ccgagttcca
    22981 acgaacgttg acgctggcgg cacccccggc agcgtcaagg cgacgcttgc cgatagctgc
    23041 tgggcgggat gccgtagtgc tcgcgaaaac tgcggctgaa gtggctggag tctcgaaaac
    23101 cattgcgcag gccgatttcc gtcacgctca gatggcgcag cctgtcgctt tccagttctt
    23161 cgcgggcatg ctgcagccgc aattgccgga gataggcgct gtactgctgc ccgtgcgatt
    23221 cgaacaagcg gtgaacccag cgcttggaca ccttgagctc gtgtgcgatc cgttccacgg
    23281 agaggttggc gtcatccagg ttggcgtcga tgaaggcgac caggcgcagc cagcgatcgg
    23341 tttcggtgtc ggacgaagat tccgctgccg agacgctttc gcaataggcc gcggccagca
    23401 gggacaccaa ggcctgctcg gcgaaataag ccgtggccgc cttcatggcc ggcagttcc
//
LOCUS       DASWPO010000262        11876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1312903, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000262 DASWPO010000000
VERSION     DASWPO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312903"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGC69_13790"
     CDS             complement(<1..266)
                     /locus_tag="VGC69_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0296397.1"
                     /translation="MSKPVILRPADQKAHERGGGARTIPLVSPRLGATSFINGITEFG
                     PGAKIPWHSHNCEESVMLLEGSAIFDIGDAHYDLQPQDITFIPP"
     gene            complement(263..1381)
                     /locus_tag="VGC69_13795"
     CDS             complement(263..1381)
                     /locus_tag="VGC69_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY0296398.1"
                     /translation="MSQTDSKTIWVGGDSLRRFIAAALRRVGLPAADAETVAGLMADA
                     DLQGSDGHGAIRLPQYVQRIESGGINVRPDIRVVHERPAMAVVDGDNGMGHLVVSRAV
                     KLAIEKARAAGVAWVGTRASNHAGPASLYARMPLEHDMIGLYFAVGNANHLPPWGGLE
                     MLLSTNPIAAAVPAGEEPAVVLDMATTVAAYGKVKARAKRGEPMPEGWMIDRNGQPLT
                     DPTRSHEGFLMPIGGYKGYGLALIVGLLAGTLQGAAMGRDVVDFNADHTTPTNTGQAI
                     LVIDLAAFGGAAPFKSAVDTLVRDIRGSERMPGVDRIWLPGEQSHERRAAYARSGAPL
                     TAALVEDLDRLASRLGIDPLARGAAPHPSESSTREGQP"
     gene            complement(1399..2772)
                     /locus_tag="VGC69_13800"
     CDS             complement(1399..2772)
                     /locus_tag="VGC69_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296399.1"
                     /translation="MALNTQVDADRDAPPVTRILAEHVARHPSRGWSDAVEHEAHRTF
                     MNWLGCAVGASRHEAGAAALRAVQALQPCAQAAVLGRAERVDMASAALVNGITSHTYD
                     FDDTHLKTIIHPAGPVCSAALALAEHTGASGRQLIDAVVLGIDVSCRVGNMIYPNHYD
                     RGWHITGTTGGLGAAAACARLLGLDAGQTMMALGIAASQPVGLREQFGTMAKPFHPGA
                     AAKAGLMSALLAQAGFTASPRAIEAPRGFAQVVSTKYDWHEITDELGERFEISFNTYK
                     PFACGIVIHPSIDACVQLRAKGVTPQNLARIDLRVHSLVLELTGKKEPADGLQGKFSV
                     YHGCAAGLIFGRASEDEYADDIVARPDVVEVRRKVVATVDDGIGEASVDATATLADGS
                     QVRVFVEHAIGSLQRPMSDADLEAKFRGMSNAILGAARTGELLAACWKLGQAADARAL
                     VRLASAA"
     gene            complement(2834..3808)
                     /locus_tag="VGC69_13805"
     CDS             complement(2834..3808)
                     /locus_tag="VGC69_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296400.1"
                     /translation="MHRRSLLSAASAMLVLPRAVLAEEKYPAYPVTYIVPVAPGGGSD
                     FVGRSVAAAWGQDLGANFIVENQAGGGGVIACEKTMHAKPDGYTLMQGYVATLATSPA
                     TRTLSYDPLRDFTPVGMIGGTPNVLVVNAKLPVHDLKEFVAYVKEHDSSISYGSAGTG
                     SLTHLLMELLQQRVGTHMVHIPYKGIEPAFTDLLSQQTQAMFPGLAAAVPHLRAKAVR
                     ALALTGPARHPNYPDVPTFQELGLGGFEDVLQWYGVSGPAGMPGAVVNRLNQSLNKVL
                     ADPGLSKKLDVEAIMPMPMSPAQFADYARRDYERWRQVAKAQDIHLDS"
     gene            complement(3900..4601)
                     /locus_tag="VGC69_13810"
     CDS             complement(3900..4601)
                     /locus_tag="VGC69_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296401.1"
                     /translation="MDWLFNPIAENEFEELRNTSLGKLVRDQLLGQIMSGVFTPGQAL
                     READLVDRLKVSRVPVREALRELESVGLVVSRKHSGVYVRELSDTEVRDLYQFRALLD
                     SNAGRAVAQLPAPRRDELARALDAQTQVMDNAIRERNPQAYYRANLQFHWLTIDYAGN
                     KEIAKAYRELIQKLHLARLKGLSDQAHREQSNAEHKRIVRALREVAASADAQRLAGLL
                     ADHVHNAYERLSDME"
     gene            complement(4770..5726)
                     /locus_tag="VGC69_13815"
     CDS             complement(4770..5726)
                     /locus_tag="VGC69_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0296402.1"
                     /translation="MTVKIALEEHFMTPGFDEYSKSFTSLMTPAALKSLSSQLADFDE
                     IRIGAMDQAGIQQVILSQTMPGVQGERDERAAVERARQSNDFLAGQVRRHPDRFAGFA
                     ALPMQDPKAATAELRRAVLELGMKGALVNGHTHGVYYDDRAFDPVWATLQELGVPLYL
                     HPLYACETAKVFEGHPELTGAIWGWGVETGTHALRLLFGGVFDRFPDLKIILGHMGEG
                     LPFLRWRFDSRFAVYSHGIELDRKPSEYFGSNILITTAGVCSAPTLMAAIAEMGREAV
                     LFSVDYPYESSSIAAGFIEAAPMDAETRELVCHGNARRIFKL"
     gene            complement(5744..6979)
                     /locus_tag="VGC69_13820"
     CDS             complement(5744..6979)
                     /locus_tag="VGC69_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEY0296403.1"
                     /translation="MEVMIIGGGIGGLSLALALHKVGVECRVYEQAPEIRALGVGVSL
                     LPHSTKVLGELGLNEAIADVAVTTSQSMFFNRFGQEVFAEKTGRNAGYDWPQFQVHRG
                     DLHLILYQACKERIGADRIVTGHRCVGFTQAGDSVAAHFTDPEGKALPSRTAPVLIAC
                     DGLHSAVRKQLHPNEGAPVYSGVNMWRGVTRWKPYIDGATYVRAGWLVPGKMVIYPVR
                     NKLDQDGLQLINWVAEITTPKYERRDWNRQGRLEDFIGVFDDWKFDWLDVPGMIRGAD
                     TILEFPMIDQDPLPWWTQDRVTLLGDAAHPMVPRGSNGAGQALLDANALAGILARNKD
                     VPNALKEYESVRLPATANVVLTNRKAPPDAILKEVYERTGDKPFKDIDDVISRAEMAA
                     ITDGYKRVAGYDKASLTSK"
     gene            complement(7017..8000)
                     /locus_tag="VGC69_13825"
     CDS             complement(7017..8000)
                     /locus_tag="VGC69_13825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296404.1"
                     /translation="MYALKSFIAATVAAVCALIAPASASAADTFPDHPIRIVVPFSPS
                     GGIDILARTIAQELTGQWQSGVVVENKPGASGNIGTDYAARSAPDGYTWLMTANTLVM
                     TPSLMPVNFKPKQDFTPVTEVAIGYLALVTRPGLPVKSVHDLIALAKSEPGKLNYGSP
                     GTGTPHHLAMELLKQKAGFNVVHVPYKGSAELVNGVLSGQVSLAFLPVHQALALARAG
                     KLQMLAAGGAKRTAVTPDVPSLAEASGIKDIDVDMWYALYLPAGAPAELVKKINVAVN
                     NILKEPSVAKRLADVGLAPRGSTPQELAATTGSDLSRWAQVIQLAGLKAGQ"
     gene            8119..9075
                     /locus_tag="VGC69_13830"
     CDS             8119..9075
                     /locus_tag="VGC69_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296405.1"
                     /translation="MNVSHRQLKAFILIARLRNFSRASEQLHIAQSGLSLMIRELETQ
                     LGFRLFNRTTRQVSLTPFGSEFLRVAEQNVENLEQAVDRVGKLAKQANTSLSIGAPPL
                     TSSYVLPEAIAAFQEKHPDIRVTVVDTDIVTVSNMVREGQLDLGMGMFLKATPSTVRE
                     PLFRFSLVMASAAPSFQLSRAPRSWASVAGSDFICLPYDNPLQQLIDGYLLRAGHNRA
                     PRFVVNFIETQLGLAAAGCGVAILPSTAIAACRGHTVRIQPLSEPLLDLDFYSLRDRS
                     RESQPYASEFCETVRRCLPHFVAPSLAAASGKKRKRQAPTAA"
     gene            complement(9101..9739)
                     /locus_tag="VGC69_13835"
     CDS             complement(9101..9739)
                     /locus_tag="VGC69_13835"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY0296406.1"
                     /translation="MFTGLVAAVGRIANVTPLPGGEQAGVRLKIEAGGLDMGNVGLGD
                     SIAVQGACMTVIALPAPGQFEVDVSQESLRRTVGLDQAGEVNLEKSLCVGDQIGGHLV
                     SGHVDGLGEVVSFEAVGESRELIVRIPAALARYLAYKGSVTVNGISLTVNRVQDSAAG
                     CEISINIIPHTQEVTTLRNVHAGDQVNIEIDTIARYVERMLSTSSSGRNALE"
     gene            complement(9789..10931)
                     /gene="ribD"
                     /locus_tag="VGC69_13840"
     CDS             complement(9789..10931)
                     /gene="ribD"
                     /locus_tag="VGC69_13840"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0296407.1"
                     /translation="MPASDTSDLACMRRALDLARSVLYITSPNPRVGCVIVRDGQVLG
                     EGATQPPGGPHAEIGALRDAQARGNSVEGATFYVTLEPCSHYGRTPPCVDALLAARPA
                     RVVVAMGDPNPQVNGRGLARLREAGIEVDVGLCLEEALALNPGFVARMRRGTPWAWLK
                     LAASLDGRSALHNGMSQWITGPEARADGHHWRARSCVVLTGMGTVLADDPQLNVRAVE
                     TPRQPRKAVVDGRFAIPENARLFDGADVLVFTARSDPAKAERLARKHVQTILMPGADP
                     LRVDLPGMMRWLGEHEVNEVHVEAGAGLSGALLSAACIDELLVYLAPVLLGDAAGMVQ
                     LPMLEHLDAAQRFEFTEMQRLGADARLRARLAQRWQALRQGLDASA"
     gene            11108..11383
                     /locus_tag="VGC69_13845"
     CDS             11108..11383
                     /locus_tag="VGC69_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY0296408.1"
                     /translation="MLELRPNCECCDRDLPPDSPDARICSFECTFCAHCAEHRLHGRC
                     PNCAGELLARPRRPAGKLAANPPSTRRVHNPDCAPSPTSFSVTRPQS"
     gene            11392..>11876
                     /locus_tag="VGC69_13850"
     CDS             11392..>11876
                     /locus_tag="VGC69_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0296409.1"
                     /translation="MKQALIAIDIQESFRQMPFWNDAELPAFIRAAQGLVDACAAHGI
                     PILQVFHIHGPEKPASVFTRDSGYVKTLAELKMPQPAEVFHKKVHSAFYGLSDQGVTL
                     HAWLRGHGIEEVIICGIRTEQCCETTARHASDDGFKVKYAMDATLTFAMTSPSGRVYS
                     P"
BASE COUNT         1940 a         3975 c         3925 g         2036 t
ORIGIN      
        1 ttcggcggga tgaaggtaat gtcctgcggc tggaggtcgt aatgcgcgtc gcctatgtcg
       61 aaaatggccg agccttccag cagcatgacg ctctcttcgc agttgtggct gtgccagggg
      121 attttcgcgc cgggcccgaa ctcggtgatg ccgttgatga agctggtggc gcccaggcgc
      181 ggcgacacca gcgggatggt gcgcgcgccg ccgccgcgct catgggcttt ctggtcggcg
      241 ggacgcagga tgacgggctt gctcatggtt gaccctctcg tgtgctggat tcggatggat
      301 gcggcgccgc gccgcgcgcc agcggatcga tgcccagccg gctcgccagc cggtccaggt
      361 cctcgaccag cgcggcggtg agcggcgcgc cgctgcgggc gtaggccgcc cggcgttcat
      421 ggctttgttc gccgggcagc cagatgcggt cgacgcctgg catgcgctcg ctgccgcgga
      481 tgtcccgcac cagcgtgtcc accgcggact tgaagggcgc ggcgccgccg aacgcggcca
      541 ggtcgatgac caggatggcc tggccggtat tggtgggcgt ggtgtggtcg gcattgaaat
      601 ccacgacgtc gcggcccatc gcggcgccct gcagggtgcc agcgagcagg cccacgatca
      661 aggccagccc atagcccttg tatccgccga tcggcatcag gaaaccctcg tgcgagcggg
      721 tcgggtcggt caggggctgg ccgttgcggt ctatcatcca gccttccggc atcggctcgc
      781 cccgcttggc ccgggccttg accttgccgt aggcggccac ggtggtggcc atgtcgagca
      841 ccaccgccgg ctcctcgccg gccgggacgg cggcggcgat cgggttggtg gacagcagca
      901 tttccagtcc gccccagggc ggcaggtgat tggcgttgcc gaccgcgaag tacagcccga
      961 tcatgtcgtg ctcgagcggc atgcgcgcat agagcgacgc gggcccggcg tggttgctgg
     1021 cccgcgtgcc cacccaggcg acgccggccg cgcgggcttt ttcgatcgcc agcttgacgg
     1081 cgcgcgacac caccaggtgg cccatgccgt tgtcgccgtc gaccaccgcc atcgccggcc
     1141 gctcgtgcac cacacggatg tccgggcgca cattgatgcc gcccgattcg atgcgctgca
     1201 cgtattgcgg cagccggatc gccccgtggc cgtccgaacc ctgcaggtcg gcgtcggcca
     1261 tcaggccggc caccgtctcg gcgtcggccg ccggcagccc cacgcggcgc agcgcggcgg
     1321 cgatgaagcg ccgcaggctg tcgccgccga cccagatggt cttgctatcc gtttggctca
     1381 tgatgaccgc cccctctctc aagccgcgct ggccagccgg accagcgctc gcgcatccgc
     1441 cgcctggccc agcttccagc aagctgcgag cagttcgccg gtccgcgccg cgcccaggat
     1501 ggcattggac atgccgcgga atttggcctc caggtcggca tcgctcatgg ggcgctgcag
     1561 cgaaccgatg gcatgctcga cgaagacgcg cacctggctg ccgtcggcca gcgtggccgt
     1621 cgcgtcgacg ctggcctcgc cgatgccgtc gtcgacggtg gcgacgacct tgcggcggac
     1681 ctcgaccacg tcgggccgcg cgacgatgtc gtcggcgtac tcgtcctcgg acgcgcgccc
     1741 gaaaatcagc ccggcggcgc acccgtgata gacgctgaac ttgccttgca ggccgtcggc
     1801 gggctctttc ttgcccgtca gttccagcac cagcgaatgc accctcagat cgatgcgcgc
     1861 caggttctgc ggggtcacgc ctttggcgcg cagctgcaca caggcgtcga tgctggggtg
     1921 gatgacgatg ccgcaggcaa agggcttgta ggtgttgaac gagatctcga agcgttcgcc
     1981 cagctcgtcg gtgatttcgt gccagtcgta cttggtcgag accacctggg caaagccgcg
     2041 cggcgcctcg atggcgcgcg gactggcggt gaagcccgcc tgggccagca aagccgacat
     2101 caggccggcc ttggccgccg cgcccggatg gaaaggcttg gccatggtgc cgaactgctc
     2161 gcgcagtccc accggctggg aagccgcgat gccgagcgcc atcatggtct ggccggcgtc
     2221 caggcccagc agccgcgcgc aggccgcggc cgcgcccagg ccgccggtgg tgccggtgat
     2281 gtgccagccg cggtcgtagt gattcgggta gatcatattg ccgacccggc acgacacgtc
     2341 gatgcccagc accaccgcat cgatgagctg ccggccgctc gcgccggtgt gctcggccag
     2401 ggcaagcgcc gccgagcaca ccggcccggc cggatggatg atggttttca ggtgcgtgtc
     2461 gtcgaagtca taggtgtgcg aggtaatgcc gttgaccagc gcggcgctgg ccatgtccac
     2521 ccgctcggcc cgtcccagga ccgcggcctg ggcgcaaggc tgcagcgcct gcaccgcgcg
     2581 cagcgcggcc gcgcccgcct cgtgccgcga cgcgccgacc gcgcagccca gccagttcat
     2641 gaaagtgcgg tgcgcctcgt gctcgaccgc atcgctccag ccccgcgacg gatggcgggc
     2701 gacgtgctcg gccaggatgc gggtaacggg aggcgcgtcg cggtcggcat cgacctgggt
     2761 gttcaaagcc atggcaatct ctgctcggtg gtcaacggga aaaaaatcgg ctcatccacg
     2821 aggcatatcc gcgtcatgaa tcgagatgga tgtcctgcgc cttggccacc tggcgccagc
     2881 gctcgtaatc gcgccttgca taatcggcga actgggccgg ggacatgggc atgggcatga
     2941 tggcctcgac gtcgagcttc ttcgacagac cgggatcggc caggaccttg ttcagcgact
     3001 ggttgagccg gttcacgacc gcgcccggca tgcccgccgg gccggacacg ccgtaccact
     3061 gcaggacatc ctcgaagccg ccgagcccca gctcctggaa ggtcggcaca tcgggatagt
     3121 tcggatggcg cgccgggccg gtcagcgcca gggcccgcac cgccttggcg cgcaagtgcg
     3181 gaacggcggc ggcgagcccg gggaacatcg cctgggtctg ctgcgacagc aggtcggtga
     3241 aggccggctc gatgcccttg tagggaatgt gcaccatatg cgtccccacg cgctgctgca
     3301 gcagctccat cagcagatgc gtgagcgagc ccgtgccggc cgagccatag ctgatgctgc
     3361 tgtcgtgctc tttgacgtag gcgacgaatt ccttgaggtc gtgcaccggc agcttggcat
     3421 tgaccaccag cacattgggc gtgccgccta tcatgccgac cggcgtgaaa tcgcgcagcg
     3481 gatcgtagga caaggtgcgc gtggccgggc tggtggccag cgtggccacg tagccctgca
     3541 tgagcgtgta gccatccggc ttggcgtgca tggtcttctc gcacgcgata acgccgccgc
     3601 cgcccgcctg gttctcgacg atgaaattcg cgcccaggtc ctggccccag gcggcggcca
     3661 cggagcggcc gacgaagtcg ctgccgccgc cgggcgcgac gggcacgatg taagtaaccg
     3721 gatacgcggg gtatttttcc tcggccagga ccgcgcgcgg caggaccagc atggccgaag
     3781 cggccgacag caatgaacgt cggtgcatct ttgtctcctc cttccttttc aggatttttt
     3841 tggcgctgca ggggctgggc tgttgttcgg cgggcccatt accccatgtt ggacaaccat
     3901 tactccatgt cggacaacct ctcgtaggcg ttgtgcacgt gatcggccag caggccggcc
     3961 aggcgttgcg catcggccga cgccgcgacc tcgcgcaggg cgcggacgat gcgcttgtgc
     4021 tcggcgttgg actgctcccg gtgggcctga tcggacaggc ccttgagccg cgccagatgc
     4081 agtttctgga tcagttcgcg gtaggccttg gcaatctcct tattgccggc gtagtcgatg
     4141 gtgagccagt ggaactgcag attggcccgg tagtaggcct ggggattccg ttcgcggatg
     4201 gcgttgtcca tgacctgggt ctgcgcgtcc agcgcgcgcg ccagctcgtc gcgccgtggc
     4261 gcgggcaatt gcgcaacggc ccggccggca ttgctgtcga gcagggcgcg gaactgatac
     4321 aagtcccgca cctcggtatc gctcagctcg cgcacataga cgcccgaatg cttgcgcgaa
     4381 accaccagcc cgacgctttc cagttcgcgc agcgcttcgc gcaccggcac gcgcgacacc
     4441 ttcagcctgt cgaccagatc cgcttcgcgc agggcctggc ccggcgtgaa gacgccggac
     4501 atgatctgcc ccagcagctg gtcgcggacc agtttgccga gcgaggtgtt gcgcagttcc
     4561 tcgaactcgt tttcggcgat ggggttgaat agccagtcca cgggccttcc tggtcaagat
     4621 catctaattg actaattgta tacaattaga cgaaatagaa agtcaacccc ggcagagccg
     4681 ggctgcgcga aaaaagccgc caggacatca gcggctcggc acgatctggc ctatacggaa
     4741 agttctcgat atcgcgtttc ccggtcggat caaagcttga aaatccggcg ggcgttgcca
     4801 tggcagacca gctcgcgcgt ctctgcgtcc atgggcgcgg cctcgatgaa cccggccgcg
     4861 atgctgctgg attcgtaggg ataatcgacg gagaagagca ccgcttctcg gcccatctcc
     4921 gcgatcgccg ccatcagggt cggcgcggag cacacgccgg cggtcgtaat caggatattg
     4981 ctgccgaagt attcggaagg cttgcggtcc agctcgatgc cgtgggaata cacggcgaag
     5041 cggctatcga agcgccagcg caggaaaggc aagccttccc ccatatgccc caggatgatc
     5101 ttcaaatccg gaaaccggtc gaagacgccg ccgaacagca accgcaaggc atgggtgccg
     5161 gtctccacgc cccaacccca gatcgcgccg gtaagttcgg gatgaccctc gaataccttg
     5221 gcggtttcgc aggcgtagag cgggtgcagg tacagcggca cgccgagttc ctgcagcgtg
     5281 gcccacaccg gatcgaacgc gcggtcgtcg taatagacac cgtgagtgtg gccgttgacc
     5341 agcgcaccct tcatgccgag ctccagcacg gcgcggcgca attccgccgt ggccgccttg
     5401 ggatcctgca taggcagcgc cgcgaagccg gcaaagcggt ccgggtgcct gcggacctgt
     5461 cccgccagga aatcgttgct ctggcgcgcg cgttcgaccg cggcgcgctc gtcgcgctcg
     5521 ccctgtacgc ccggcatggt ctgcgacagg atcacctgct ggatgccggc ctggtccatc
     5581 gcaccaatcc ggatctcgtc gaaatccgcc agttgcgacg acaacgactt gagcgccgcg
     5641 ggcgtcatca ggctggtgaa tgacttcgaa tactcgtcga agcccggtgt catgaagtgt
     5701 tcttcgagcg caatcttgac tgtcatggga atctcctgct gctttacttt gacgtgagcg
     5761 aggctttgtc gtagcccgcg acgcgcttgt agccatcggt gatcgcagcc atctcggcgc
     5821 ggctgatcac gtcgtcgatg tccttgaagg gcttgtcgcc ggtgcgctcg taaacctctt
     5881 tcaggatggc gtccggcggc gccttgcggt tcgtcaacac cacgttggcc gtggcgggca
     5941 gccgcaccga ttcatattct ttcagggcat tgggaacgtc cttgttgcgg gcaaggatgc
     6001 cggccagcgc gttcgcgtcc aggagggcct ggccggcgcc gttggagcct cgcggcacca
     6061 tggggtgcgc cgcatcgccg agcagcgtga cgcggtcctg cgtccaccag ggcaagggat
     6121 cctggtcgat catggggaat tcgaggatgg tgtcggcgcc gcggatcatg cccggcacgt
     6181 caagccagtc gaacttccaa tcgtcgaata caccgatgaa gtcctccagg cggccctggc
     6241 ggttccagtc gcggcgctcg tatttcggcg tcgtgatttc cgccacccag ttgatgagct
     6301 gcaagccgtc ctgatcgagc ttgttgcgca ccggatagat gaccatcttg ccgggcacca
     6361 gccagcccgc gcgcacatag gtcgccccat cgatataggg tttccagcgc gtgacgccgc
     6421 gccacatgtt cacgccggag tacaccggcg cgccttcgtt cgggtgcagt tgcttgcgca
     6481 cggcggaatg gaggccgtcg caggcgatca ggaccggggc cgtgcggctg ggaagcgcct
     6541 tgccttccgg atcggtgaag tgggcggcaa cgctgtcgcc agcctgcgtg aagccgacgc
     6601 agcggtgccc ggtgacgata cggtccgcac caatgcgctc cttgcatgcc tggtacagaa
     6661 tcaggtggag gtcgccccga tgcacttgaa attgcggcca gtcgtagccg gcattccggc
     6721 ccgttttctc ggcaaacact tcctgtccga agcggttgaa gaacatcgat tgcgacgtgg
     6781 tcaccgccac gtcggcgatc gcttcgttga ggccgagctc gccgagcacc ttggtggagt
     6841 gcggcaagag gctcacgccg acgcccaagg ctctgatttc gggggcctgt tcgtacacgc
     6901 ggcattcgac gccgactttg tgcagggcga gcgccagcga gaggccgccg atgccgcctc
     6961 cgatgatcat gacttccatt tgatttcctt cgaggacctg cgggatgaat gcgttgctat
     7021 tggccggcct tgagtccggc gagctggatc acttgagccc agcgcgacaa gtccgaaccg
     7081 gtcgtggccg ccagttcctg cggcgtgctg ccgcgcgggg ccagacccac gtccgcaagg
     7141 cgcttcgcca cggacggctc tttcaggata ttgttgaccg ccacattgat cttcttcaca
     7201 agctccgcag gcgcgccagc gggaagatag agggcgtacc acatgtcgac gtcgatatcc
     7261 ttgatgccgc tggcttccgc cagggacggc acgtcaggcg tgacggcggt acgcttcgcg
     7321 ccacccgccg cgagcatctg caatttgccc gcgcgggcca gggccaatgc ctggtggacg
     7381 ggaaggaagg ccagactcac ttgccccgaa agcaccccgt tgaccagttc ggccgagccc
     7441 ttgtaaggca cgtgcacgac gttgaagccg gccttctgct tcagcagctc catggcgagg
     7501 tggtgcggcg tacctgtgcc cggggagccg tagttcagct tgccgggctc ggacttggcc
     7561 aatgcgatca ggtcatggac gctcttgacc ggcaagcccg ggcgggtaac cagggccagg
     7621 taaccgatgg cgacctcggt caccggcgtg aagtcctgct tgggcttgaa attcaccggc
     7681 atcaggctgg gggtcatcac cagcgtgttg gccgtcatca gccaggtata accatccggc
     7741 gccgaacggg ccgcgtaatc cgtgccgata ttgccgcttg cgcctggctt gttctcgacg
     7801 accacgccgg actgccattg gccggtcaac tcctgggcga tggtgcgcgc gaggatatcg
     7861 atgccgccgc tcggcgagaa cgggacgacg atcctgatcg gatggtccgg aaaggtatcg
     7921 gccgctgatg ccgatgccgg cgcgatcagg gcgcagacgg ccgcaacggt ggcggcgatg
     7981 aatgatttca gtgcgtacat ggcgcatccc cttgtgagtg aggcgtccat tccaccggaa
     8041 atcaatcaat atgataaata gaaatataat attatttaag cagaaaacca gatcaatcat
     8101 catcgcgacg gcaccgccat gaatgtttca catcggcagc tcaaggcgtt catcctgatt
     8161 gcgcgtctgc gcaatttctc gcgcgcgtcc gagcaactgc acatcgccca atccggcctc
     8221 agcttgatga ttcgcgagct ggaaacccaa ctcggctttc ggctgttcaa ccgcacgaca
     8281 cgccaggtca gcctgactcc attcggatcg gaattcctgc gggtcgccga gcagaacgtg
     8341 gaaaacctgg agcaagctgt cgatcgcgtc ggcaaactgg cgaagcaggc caacaccagc
     8401 ctgtccatcg gcgccccgcc cttgaccagt tcctatgtat tgccggaagc gatcgcggcg
     8461 ttccaggaga agcatccgga catacgcgtg acggtggtcg acacggatat cgtcacggtc
     8521 tccaacatgg tgcgcgaggg gcagctggac ctgggcatgg gcatgttcct gaaggcaacg
     8581 cccagcacgg tgcgggagcc cctattccgg ttttcactgg tgatggcgtc cgcagcccct
     8641 tcgttccagc tcagccgcgc gccgcgcagc tgggcttcgg tcgccggcag cgatttcatc
     8701 tgcctgcctt acgacaaccc gctgcagcag ctgatcgacg gatatctctt gcgggccggc
     8761 cacaaccggg cgccgcgttt cgtcgtcaat ttcatcgaga ctcagttggg cctggccgcc
     8821 gcggggtgcg gcgtggcgat tctcccttcc acggccatcg ccgcatgccg cgggcacacc
     8881 gtcaggattc agcccttatc ggaaccgctg ctcgacctgg atttctatag tttgcgggat
     8941 cgctcccgtg aatcgcagcc gtacgccagc gagttctgcg agaccgtgcg gcgctgcctg
     9001 ccgcacttcg tggcaccgtc gctcgcggcg gcctccggca agaagcgcaa gcggcaagcg
     9061 cccaccgcgg cttgaggtgg ccgctagttt tgaaatcaaa ctattccaat gcgttgcgac
     9121 cgctactgga cgtggagagc atccgctcga cataccgggc gatggtgtcg atctcgatat
     9181 tgacctggtc gccggcgtgc acattgcgca gcgtggtgac ttcctgcgtg tgcgggatga
     9241 tgttgatgct gatctcgcag ccggcggcgg agtcctgcac gcgattgacc gtgaggctga
     9301 tgccgttgac ggtgaccgac cccttataag ccaggtagcg cgccagcgcg gcgggaatgc
     9361 gcacgatcag ttcgcgcgat tcgcccacgg cctcgaagct caccacttcg cccaggccgt
     9421 ccacatggcc cgacaccagg tggccgccga tctggtcgcc cacgcacagc gatttttcca
     9481 ggttcacctc accggcctgg tccagcccca ccgtgcggcg caggctttcc tgcgacacgt
     9541 ccacttcgaa ctgcccgggc gcgggcagcg cgatcaccgt catgcaggcg ccctgcaccg
     9601 cgatcgaatc gcccaggccc acgttgccca tatccaggcc gcccgcctcg attttcaggc
     9661 gcacgccggc ctgctcgccg ccgggcagcg gggtgacgtt ggcgatgcgg cccactgccg
     9721 cgacgagacc ggtgaacatg gggagcttcc ttcaacagtg acaagggagg agtaaagagg
     9781 atgcgcgttc aggccgacgc gtccaggccc tggcgcaatg cctgccagcg ttgcgccagg
     9841 cgcgcgcgca ggcgcgcgtc ggcgccgagg cgctgcatct cggtgaattc gaaacgctgc
     9901 gccgcgtcga gatgctccag catgggcagt tgcaccatgc cggcggcatc gcccagcagc
     9961 accggggcga ggtagaccag cagttcgtcg atgcaggcgg ccgacagcag cgcgccggac
    10021 aggcccgcgc cggcctcgac gtgcacctcg ttgacctcat gctcacccag ccagcgcatc
    10081 atgccgggca ggtcgacccg cagcggatcc gcgccgggca tcaggatggt ctgcacatgc
    10141 ttgcgcgcca ggcgctcggc cttggccgga tccgacctgg ccgtaaacac cagcacgtcg
    10201 gcgccatcga acaggcgcgc gttctcggga atggcgaaac ggccgtcgac cacggccttg
    10261 cgcggctggc gcggcgtctc gacggcacgc acgttgagct gcggatcgtc ggccagcacc
    10321 gtccccatgc cggtgagcac cacgcagctg cgcgcgcgcc agtgatgccc gtcggcgcgc
    10381 gcctcggggc cggtgatcca ttgcgacatg ccgttgtgca gggcgctgcg gccgtccagc
    10441 gaggccgcca gcttgagcca ggcccagggc gtgccgcgcc gcatgcgcgc gacgaagccc
    10501 ggattgagcg ccagggcctc ttccaggcac aggccgacgt cgacctcgat gccggcctcg
    10561 cgcagcctgg ccaggccgcg gccattgacc tgggggttgg ggtcgcccat ggcgaccacc
    10621 acgcgcgccg gcctggccgc caggagcgcg tccacgcagg gcggggtgcg gccataatgg
    10681 ctgcagggct cgagcgtgac gtagaaggtg gccccttcga ccgaattgcc gcgcgcctgc
    10741 gcgtcgcgca gggcgccgat ctcggcgtgc gggccgccgg gcggctgcgt ggccccctcg
    10801 cccagcacct ggccgtcgcg cacgatcacg caacccacgc gcgggttggg cgaggtgatg
    10861 tagagcacgc tgcgcgccag gtcgagggcg cgccgcatgc aggcaaggtc tgacgtgtcg
    10921 gaagcaggca tggcctgatt gtaatgccgg gacacctgcc cgccgcggcg cccggcgccc
    10981 ggccgcggcg gtttccaggg cttgggcacg gatgtccgtt tcagtcctaa aaacgtcccc
    11041 ggcgcctctc gcgccagccc ccggcctgga tcagaattca agctgtaccc cactcacgga
    11101 aagccccatg ctggaactca gaccaaactg cgagtgctgc gaccgcgacc tgccgccaga
    11161 ctcgcccgat gcgcgcatat gctccttcga atgcacgttc tgcgcgcatt gcgccgaaca
    11221 ccgcctgcat gggcgctgcc ccaattgcgc gggcgagctg ctggcgcgcc cccggcggcc
    11281 ggccgggaag ctcgcggcca acccgccatc gacccgcagg gtgcacaatc cggactgcgc
    11341 cccctccccg acgtcctttt ccgtcaccag gccccaatcg tgaaacaagc catgaaacaa
    11401 gccctgatcg ccatcgatat ccaggaatcc ttccgccaga tgcccttctg gaacgacgcg
    11461 gagctgcccg ccttcatccg ggccgcccag ggcctggtgg acgcctgcgc cgcgcacggc
    11521 attcccatcc tgcaggtgtt ccacatccat ggcccggaaa aacccgcgag cgtcttcacc
    11581 cgcgactcgg gctacgtcaa gacgctggcc gaactgaaga tgccgcaacc cgccgaggtg
    11641 ttccacaaga aggtgcactc ggcgttctac ggcctgagcg accagggcgt cacgctgcac
    11701 gcctggctgc gcgggcatgg catcgaggag gtcatcatct gcggcatccg caccgagcaa
    11761 tgctgcgaga ccacggcgcg ccatgcgtcg gacgacggct tcaaggtgaa gtacgccatg
    11821 gatgccaccc tgacctttgc catgacgagc ccgtccggcc gcgtctactc gccgca
//
LOCUS       DASWPO010000263         7253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1313082, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000263 DASWPO010000000
VERSION     DASWPO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313082"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VGC69_13855"
     CDS             <1..669
                     /locus_tag="VGC69_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296410.1"
                     /translation="HQGPRQRVALARALVYEPSIMLLDEPLSALDKKLRTDLQWELRK
                     LNKESGASFLYVTHDQEEALSMSDRIVIFNHGRIAQIGTPSQLYEHPADLFVAGFLGR
                     SNVLDGVVRDAAPDSLYLDYEGGVLGVPRVGAWSAGDRVSVAVRPHRLRLRAAGQGLR
                     AKVEAISYVGNLMHYLLRTPAGAPLYAEHVAGDEMAPAPVPGDWVGVVFDAAHVSVLE
                     PRTP"
     gene            757..1206
                     /locus_tag="VGC69_13860"
     CDS             757..1206
                     /locus_tag="VGC69_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0296411.1"
                     /translation="MKFADLREGLVIKAGPVKVTREEVLEFARRYDPQWFHADPQRAE
                     EGRWGGLIASGWHTCSLAMRMAVDAILHDSESFGSPGLGEVRWRIPVRPGDELRLEAQ
                     VQAARKSSSRDDLGIVSWAWVVFNQHGEAVLELNVTSLFDLSGGTSV"
     gene            complement(1213..2211)
                     /locus_tag="VGC69_13865"
     CDS             complement(1213..2211)
                     /locus_tag="VGC69_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0296412.1"
                     /translation="MMGKLHMWKAAALVAAALALPAAHPAWAATTLTMTTEYPATSMP
                     GLGVSTFADLVNKKTKGQVVIDASYDAGKGIKSGGMIDAVQARKVDAGDAFAGALATR
                     YPIFGVSSLPFLADSLKKARALTHASRPAYEKLLAEHGQKLLYTTPWPASGIWSKKPV
                     RSVADLQALTIRTYDDTSQQVMGKAGAKGVNISFADAMPRIASGEVNAVLSSGDGGAG
                     RKLWDYLPCFAEINYAMPISIATMNLDAYNALDAASRKAIDQAAAQTESAQWTRIQGR
                     LKENYANMRKNGVKIDTRVPASVKKALEAASTSSLGDWKTATGADGAALLNRYNKR"
     gene            complement(2319..3512)
                     /locus_tag="VGC69_13870"
     CDS             complement(2319..3512)
                     /locus_tag="VGC69_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0296413.1"
                     /translation="MSENPSFNWQDPLLLDQQLTEEERMVRDAAAAYAQDKLAPRVLE
                     AFRHEKTDPAIFTEMGELGLLGPTIPTEYGGSGLNYVSYGLIAREVERIDSGYRSMMS
                     VQSSLVMVPINEFGSEAQKQKYLPKLARGEWIGCFGLTEPNHGSDPNGMETRAVKVDG
                     GYSLSGNKMWITNSPIATVFVVWAKCVGGEHDGKIRGFILEKGMKGLSAPAIHGKVGL
                     RASITGEIVMDEVAVGEEQMLPKVSGLRGPFTCLNSARYGIAWGALGAAEFCWHTARQ
                     YTMDRKQFGRPLAQNQLIQKKLADMQTEITLALQGCLRLGRMKDEGTAATEITSIMKR
                     NSCGKSLDIARLARDMLGGNGISDEFGVARHLVNLEVVNTYEGTHDVHALILGRAQTG
                     LQAFF"
     gene            complement(3956..5638)
                     /locus_tag="VGC69_13875"
     CDS             complement(3956..5638)
                     /locus_tag="VGC69_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0296414.1"
                     /translation="MTSTPTPRLGGHILVDQLVAQGVKHVFCVPGESYLAVLDGLHDA
                     RIEVTVCRQEGGAAMMAEAHGKLTGEPGVVMVTRGPGASNALAGIHIAKQDSTPLIMF
                     VGQIERGMRDREAFQEMDYRAVFGTQTKWATEIDQVERIPELVSRAFHVATSGRPGPV
                     VIALPEDMLVETAQVADAPRYEVIDSAPAPGQIDELARLLAAARSPMAIVGGSRWNEQ
                     AVAQFAAFAEKHALPVAVSLRRQMLFPADHPCFIGDVGLGINPALAERVKSADLVLLV
                     GGRMSESPSQSYELFDIPVPSQKLVHVHPDSSELARVYRANLAICASPIAFAAALAQV
                     PAPAVEPAWAAGTQTARATYLKWSDPAGIRTLGKLQMGEVMAHLEGVLPADAIMTNGA
                     GNFATWLHRFHKFTRYGTQLAPTSGSMGYGLPAAVGAKRLYPKKTVVCFAGDGDFLMH
                     GQEFATAVQYGLPIVVLIIDNGMYGTIRMHQERHYPGRVSATGLQNPDFAAYAAAFGG
                     HGERVESTAEFAPAFERALASGKPAILHCLIDPEVISPGTTLEKIRNAALKG"
     gene            5761..6654
                     /locus_tag="VGC69_13880"
     CDS             5761..6654
                     /locus_tag="VGC69_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296415.1"
                     /translation="MRNGIPNLSALQAFEASARLGSFSRAAEELALTHSAVYRQVAGL
                     ESRLGVQLFTRVRRRIVLTEPGAEYAGRIRHHLDQIEKDTSGLVSRTGMGRSIHVAVV
                     PTLATSWLIPRLADFQRLHPDITVSLSARTLPFQFKDQPFDAALYHGDGIWPGTQGVL
                     LFPERELAPVCAPPLAERIRGAGAGLAGVTHLHLSSRPDAWRHWYESNQFVYGPQAAG
                     GPRYELFTMVLASVAAGLGVALIPRFLAQPALQAGTLVMPVPQALVVGQGYYFGYPQH
                     NERSDALKVFEAWLKAEAASP"
     gene            6651..>7253
                     /locus_tag="VGC69_13885"
     CDS             6651..>7253
                     /locus_tag="VGC69_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0296416.1"
                     /translation="MSDTARPALAAQADIDAFIDAVWLEDGLAANTLAAYRRDLAAFA
                     GWLETPAASRLPSAIALSQAEAAHIEAWFAARHAESRPTTANRRLAALRRFYAWALRE
                     HRSPRDPCLTLVAAKQPPRLPKTLSEAQVEALLRAPDTDTARGLRDRAMLETLYATGL
                     RVSELVGLRALDVSLNEGVVRVVQGKGGKDRLVPLGAEAAH"
BASE COUNT         1075 a         2356 c         2570 g         1252 t
ORIGIN      
        1 caccagggcc cgcgccagcg cgtggcgctg gcgcgggccc tggtgtatga gccgagcatc
       61 atgctgctcg acgaacccct ctccgcgctc gacaagaaat tgcgcacgga cctgcaatgg
      121 gagctgcgca agctcaacaa ggaatcgggc gcttcgttcc tgtatgtgac gcacgatcag
      181 gaggaggcct tgtccatgtc ggaccgcatc gtcattttca accatggccg cattgcgcag
      241 atcggcacgc cgtcgcagct ctacgagcat ccggccgatc tgttcgtggc cgggttcctg
      301 ggacgcagca atgtgctcga cggggtcgtg cgcgacgccg cgccggatag cctgtacctg
      361 gattacgaag gcggcgtgct gggcgtgccg cgcgtgggcg cctggtccgc cggcgaccgg
      421 gtcagcgtgg cggtgcggcc gcaccgcctg cgcctgcgcg ccgccgggca ggggctgcgc
      481 gccaaggtgg aggcgatcag ctatgtgggc aatctcatgc attacctgct gcgcacgccg
      541 gccggcgcgc ccctgtacgc ggaacatgtt gccggagatg agatggcgcc cgcgcctgtg
      601 ccgggcgact gggtgggtgt ggttttcgac gcagcgcatg tcagcgttct ggagccgcgc
      661 accccatgag gactgtccgg gccgccgggt ttggtctatt cttttcgctt cttaagagag
      721 aatggccggc cctggcaccg gataagcggg gagacgatga aattcgccga tttgcgtgaa
      781 ggcctggtga tcaaggccgg gccggtcaag gtcacgcggg aagaagtcct ggaattcgcg
      841 cgcaggtacg atccgcagtg gttccatgcc gatccgcagc gcgccgagga aggccgctgg
      901 ggcggcctga tcgccagcgg ctggcacacc tgctcactgg ccatgcgcat ggcggtcgac
      961 gccatcctgc acgattccga atcgttcggc tcgcccgggc tgggcgaggt gcgctggcgc
     1021 attccggtgc gccccggcga cgaactgcgc ctcgaagccc aggtgcaggc ggcgcgcaag
     1081 tcgtcctcgc gcgacgacct gggcatcgtc agctgggcct gggtcgtctt caaccagcac
     1141 ggcgaggccg tgctggagct caatgtcacc agcctcttcg acctgagcgg cgggacgtcg
     1201 gtctgaacac gcctagcgct tgttgtagcg gttgagcagg gcggcgccgt ccgcgcccgt
     1261 ggcagtcttc cagtcgccca gcgacgaagt cgaggcggcc tcgagcgcct ttttcacgga
     1321 tgccggcacg cgggtatcga tcttcacgcc gttcttgcgc atattggcgt agttttcttt
     1381 gagccgtccc tggatgcgcg tccattgggc gctttcggtc tgggcggcgg cctggtcgat
     1441 ggccttgcgg ctggcggcgt ccagggcgtt gtaggcatcc aggttcatgg tggcgatcga
     1501 gatcggcatg gcatagttga tctcggcgaa gcagggcagg tagtcccaca gcttgcggcc
     1561 cgcgccgcca tcgcccgagg acagcacggc attgacctcg cccgaggcga tgcgcggcat
     1621 ggcgtcggca aacgagatgt tcacgccttt ggcgcccgcc ttgcccatga cctgctgcga
     1681 ggtgtcgtca taggtgcgga tggtcagcgc ctgcaggtcg gccacgctgc gcaccggctt
     1741 cttggaccag atgcccgagg cgggccaggg cgtggtgtag agcagcttct ggccgtgctc
     1801 ggccagcagt ttttcatagg ccgggcgcga ggcgtgggtg agggcccggg cttttttcag
     1861 cgagtcggcc agaaagggca gggaagacac gccgaagatg gggtagcgcg tggccagcgc
     1921 gccggcaaag gcgtcgccgg cgtccacctt gcgggcctgc acggcgtcga tcatgccgcc
     1981 ggacttgatg cccttgccgg cgtcgtagct ggcatcgatg acgacctggc ccttggtctt
     2041 tttgttgacc aggtcggcaa aggtcgacac gcccaggccg ggcatggacg tggccgggta
     2101 ctcggtggtc atggtcaggg tggtggcggc ccaggccggg tgggccgccg gcagcgccag
     2161 ggcggcggcg accagtgcgg cggctttcca catatgcagc ttgcccatca tgtctcctcg
     2221 gttgatgaaa aatgcctcgt gcgcgggaaa gcgcaggagg cattctggca gaagcgggca
     2281 agccgcgcga cgggattatc cctcgcgggc ggcaggcatc agaagaaggc ctgcaggccc
     2341 gtctgggcac ggcccaggat cagggcgtgc acgtcgtggg tgccttcgta ggtgttgacc
     2401 acttccaggt tgaccaggtg gcgggccacg ccgaactcgt cggaaatgcc gttgccgccc
     2461 agcatgtcgc gcgccaggcg ggcgatgtcc agcgacttgc cgcaggagtt gcgcttcatg
     2521 atcgaggtga tttccgtcgc ggcggtgcct tcgtccttca tgcggcccag gcgcaggcag
     2581 ccctgcaggg ccagggtgat ctcggtctgc atgtcggcca gcttcttctg gatgagctgg
     2641 ttctgggcca gcggacggcc gaactgcttg cggtccatgg tgtattggcg ggcggtgtgc
     2701 cagcagaatt cagccgcgcc cagcgcgccc caggcgatgc cgtagcgggc cgagttcagg
     2761 caggtgaagg ggccgcgcag gccgctgacc ttgggcagca tctgttcttc gcccaccgcg
     2821 acttcgtcca tgacgatctc gccggtgatc gaggcgcgca ggccgacctt gccgtggatg
     2881 gccggggccg acaggccctt catgcccttt tccaggatga agccgcggat cttgccgtcg
     2941 tgctcgccgc ccacgcactt ggcccacacc acgaacactg tggcgatggg cgagttggtg
     3001 atccacatct tgttgccgga gagcgagtag ccgccgtcga ccttgactgc gcgggtttcc
     3061 atgccgttgg ggtcggagcc gtggttgggt tcggtcaggc cgaagcagcc gatccattcg
     3121 ccgcgggcca gcttgggcag gtacttctgc ttttgcgctt cgctgccgaa ttcgttgatg
     3181 ggcaccatta ccagggacga ctgcacgctc atcatcgagc ggtagcccga gtcaatgcgc
     3241 tcgacctcgc gggcgatcag gccgtagctg acgtagttca ggcccgagcc gccgtattcg
     3301 gtggggatgg tggggccgag caggcccagc tcgcccattt cggtgaagat ggccgggtcg
     3361 gtcttttcgt ggcggaaggc ctcgagcacg cgcggggcca gcttgtcctg ggcgtaggcc
     3421 gccgcggcat cgcgcaccat gcgttcttct tcggtgagct gctggtccag cagcaggggg
     3481 tcttgccaat tgaaagaggg attttcggac atggtgtgct gtctctggtt gagtgattga
     3541 attagtttag ggttaaagca attgaatagg caagctgggg gtttttaaat cgttgatgtg
     3601 cgttcttaag tccgtcctgc ggtcggcggt gacagtctgc ggggcttcgc tgtgccgggc
     3661 tcgggcgcat gcaggcgccc tcggtcccct gcggggactg ccccgtcaca gaggcgcccc
     3721 gcaggctgtc accgccgacc gcaggactac ataaggccat gcgacgggtt gaagccgttg
     3781 catgactctg gtgcagtttc ccgggtgtcg ggcctgcccc aggggcgcct ctgtgacggg
     3841 gcagcccgcg cagcgggccg agggcgcctg catgcgcccg agcccggcac agcgaagccc
     3901 ctggggcagg cccgacaccc gggaaacgga tcaagaactc aaaaagcgat tttcatcacc
     3961 ccttcaacgc cgcattacgt atcttctcca gcgtggtgcc cgggctgatc acctcgggat
     4021 cgatcaggca gtgcaggatc gccggcttgc cgctggccag ggcgcgttcg aaggccggcg
     4081 cgaattccgc ggtgctctcg acccgctcgc catggccgcc gaatgccgcg gcataggccg
     4141 cgaaatcggg attctgtagg ccagtggccg agacgcggcc cgggtagtgc cgctcctggt
     4201 gcatgcggat ggtgccgtac atgccgttgt cgatgatcaa gacgacgatg ggcaggccgt
     4261 actgcacggc ggtggcgaat tcctggccgt gcatcaggaa gtcgccgtcg ccggcgaagc
     4321 acaccacggt tttcttcgga tagaggcgct tggcgcccac ggcggccggc aggccgtagc
     4381 ccatcgagcc cgaagtgggc gcgagctgcg tgccgtagcg cgtgaacttg tggaagcgat
     4441 gcagccaggt ggcgaagttg cccgcgccat tggtcatgat ggcatcggcg ggcaggacgc
     4501 cttcgaggtg cgccatgacc tcgcccatct gcagcttgcc cagcgtgcgg atgccggcgg
     4561 ggtcgctcca cttcaggtag gtggcgcgcg cggtttgcgt gccggcggcc caggccggct
     4621 ccacggccgg cgcgggcact tgcgccagcg ccgcggcaaa cgcgatgggc gaggcgcaga
     4681 tggccaggtt ggcgcgatac acgcgggcca gttcgctgct gtcgggatgc acgtgcacca
     4741 gcttctggct gggcacgggg atgtcgaaaa gctcgtagga ctggctgggc gattccgaca
     4801 tgcgcccgcc caccagcagc accaggtcgg ccgatttgac gcgctcggcc agcgccgggt
     4861 tgatgcccag gcccacgtcg ccgatgaagc aggggtggtc ggcggggaac agcatctgcc
     4921 ggcgcagcga gacggccacg ggcaacgcgt gcttttccgc gaaggcggca aattgcgcca
     4981 cggcctgttc gttccagcgc gagccgccca cgatcgccat gggcgacctg gccgcggcca
     5041 gcagacgcgc cagttcgtcg atctggccgg gcgcgggggc gctgtcgatc acttcgtagc
     5101 gcggggcgtc ggcgacttgc gcggtttcga ccagcatgtc ttcgggcagg gcgatcacca
     5161 ccgggccggg gcggcccgag gtcgccacat ggaaggcgcg cgagacgagt tcggggatgc
     5221 gttcgacctg gtcgatctcg gtggcccact tggtctgcgt gccgaacacg gcgcggtaat
     5281 ccatttcctg gaaggcttcg cggtcgcgca tgccgcgctc gatctggccc acgaacatga
     5341 tgagcggggt cgaatcctgt ttggcgatgt ggatgccggc cagggcgttc gaggcgcccg
     5401 ggccgcgcgt gaccatcacc accccgggct cgccggtgag cttgccgtgc gcctcggcca
     5461 tcatggccgc gccgccttcc tggcggcaga cggtcacctc gatcctggcg tcgtgcaggc
     5521 cgtcgagcac ggccagatag ctctctcccg ggacgcagaa cacgtgtttg acgccttgcg
     5581 cgaccaactg gtcgaccagg atgtgcccgc ccaggcgggg agtgggagtg gaagtcatgt
     5641 aggtgaacaa gggtgcagac agggaatcgg acgagcataa tgtgtctgca atgcacaaag
     5701 caattgataa aattgcacaa tcttttgatt cgccggcacg tgcccggcac ggttcgtccc
     5761 atgagaaacg gcattcccaa cctgagcgcg ctgcaggcct tcgaggcctc cgcccggctg
     5821 ggcagcttct cgcgcgcggc cgaggagctg gcgctcaccc acagcgccgt ctaccgccag
     5881 gtggccggcc tggagtcgcg gctgggcgtg cagctcttca cccgcgtgcg ccgccgcatc
     5941 gtgctcaccg agcccggcgc cgaatatgcc ggccgcatcc gccatcatct cgaccagatc
     6001 gagaaagaca cctccggcct ggtcagccgc acgggcatgg gccgcagcat ccacgtggcc
     6061 gtggtgccca cgctggccac cagctggctg atcccgcgcc tggccgactt ccagcgcttg
     6121 catcccgaca tcaccgtcag cctgtcggcg cgcaccctgc ctttccagtt caaggaccag
     6181 cccttcgacg ccgcgctcta ccatggcgac ggcatctggc ccggcacgca gggcgtgctg
     6241 ctgtttcccg agcgggagct ggcgccggtg tgcgcgccgc ccttggccga gcgcatacgc
     6301 ggcgcgggcg cgggcctggc cggcgtgacg catctgcacc tgagctcgcg ccccgatgcc
     6361 tggcgccatt ggtacgaatc caatcaattc gtctatgggc cgcaggcggc cggcgggccg
     6421 cgctacgaac tcttcaccat ggtgctggcc tcggttgcgg ccggcctggg cgtggcgctg
     6481 atcccgcgct ttctggccca gccggccctg caggccggca cgctggtcat gcccgtgccg
     6541 caggcgctgg tggtggggca gggctattac ttcggctacc cgcagcacaa cgaacgctcc
     6601 gacgcgctca aggtgttcga ggcctggctg aaggcggagg ccgcctcgcc atgagcgaca
     6661 ccgcccggcc ggcgctggcc gcgcaggccg acatcgacgc gttcatcgac gccgtctggc
     6721 tggaggacgg cctggccgcc aacacgctgg ctgcctaccg ccgcgacctc gcggcctttg
     6781 ccggctggct ggagacgccc gccgccagcc gcctgccgtc cgccatcgcg ctgagccagg
     6841 ccgaggcggc gcatatcgag gcctggttcg ccgcgcgcca tgcggaaagc cggcccacca
     6901 ccgccaaccg tcgcctggcc gcgctgcgcc gcttctatgc ctgggcgctg cgcgagcatc
     6961 gcagcccgcg cgatccttgc ctgaccctgg tggccgccaa gcagccgccg cggctgccca
     7021 agacgctgtc cgaagcgcag gtggaggcct tgctgcgggc gccggacacg gacaccgccc
     7081 gcggcctgcg cgaccgcgcc atgctggaga cgctctacgc cacgggcctg cgcgtatccg
     7141 agctggtggg cctgcgcgcg ctcgatgtca gcctgaacga aggcgtggtg cgcgtggtgc
     7201 agggcaaggg cggcaaggac cgcctcgtgc cgctgggcgc ggaagccgcg cac
//
LOCUS       DASWPO010000264         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3189440, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000264 DASWPO010000000
VERSION     DASWPO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3189440"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VGC69_13890"
     CDS             <1..645
                     /locus_tag="VGC69_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296417.1"
                     /translation="RARPQEDELVITTLQSFARHRLIARLPGFQRAHPHYRIRLQTGL
                     ELSELQQGMADIAIRMGQGPWPGLAQQPLLEDESLVVAAPHFAGGKLPRTPREVIRCP
                     LIDETDGTWSDWCQAAGVPEPAHAARITANDSNVSVGCAELGHGLLLARRSIVAGLLA
                     EGRLVQVTKVTIPYPYPYWLVWPQRDTLPEKVRHFTEWISGEMARFTQEAATER"
     gene            733..1014
                     /locus_tag="VGC69_13895"
     CDS             733..1014
                     /locus_tag="VGC69_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296418.1"
                     /translation="MSMKKTDLEKNKALKLTHSLKQARSDRFGKGAADAPLDRREQRK
                     LDQAKGLVPFACKLNSELIGTLNERAAAHPEGMTGLLDELLRHALAAKP"
     gene            complement(1087..3168)
                     /locus_tag="VGC69_13900"
     CDS             complement(1087..3168)
                     /locus_tag="VGC69_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0296419.1"
                     /translation="MSASDTPIGHGLNPAQKEAVLYLNGPCLVLAGAGSGKTRVITQK
                     IAYLLRECGYMGRNVAALTFTNKAAREMAERVKTLVDPKLAKGLTISTFHALGVRFLR
                     EEAAHAGLKPQFSILDSDDAMGIIQDLLATTDRGRLREVQSIISLWKNQLITPDQAAQ
                     AAKTPSEVEAVQVYRSYAATLAAYQAVDFDDLIRIPAEVLERDEAVRTRWQNRIRYLL
                     VDEYQDTNACQYRLVQLLAGMRAMFTAVGDDDQAIYAWRGATVENLAKLTADYPQLKL
                     IKLEQNYRSVQRILAAANSVIARNPKLFEKKLWSDLGAGEPITVSAMDGEEQEAENVA
                     MQISASRFERRSQWKDYAILYRGNHQSRILEQALRNVKIPYTISGGQSFFDKAEVRDI
                     LAYLRLIANDEDDPAFIRAVTTPKRGIGQSTLQTLGQYASSRGVSMLAAVAESGLESQ
                     LAARQLEPLRTFTEFVRRIQWRAGRGAETAQRGGGIQSAEPAGVILDDLLGAIQYERY
                     LYDTLEERPAQNRWQNVLELTGWLKRKAEEDGMTLLDLVQHVALVTMLERGDDEEPDA
                     VKLSTLHASKGLEYPHVYLAGVEEGLLPHLGKDDEDTGDPAKAAETLAVRIQEERRLM
                     YVGITRAQRSLHLSWCKKRRRAREDVVREPSRFIEEMGLGQPGIAEDEETKAMSPKQR
                     LDMLKALLNKS"
     gene            complement(3168..5309)
                     /locus_tag="VGC69_13905"
     CDS             complement(3168..5309)
                     /locus_tag="VGC69_13905"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0296420.1"
                     /translation="MTQPAPDPSPAGPSDPSPSWLRVALDVPLPGLFDYRSDQAVEPG
                     LRVIVPFGRRKLVGVVVEQTDAPTIDPALVKPVEQVLRDLPPFSSDWMRLASFAAEYY
                     QRPLGEVMLPVLPPPLRKPSAYQGKRSGQGPVARRDASQSKRAGKATDTADISDASDA
                     VAAAVSRPPGLHAEQRAAVDAIDALRAFKPVLLHGVTGSGKTEVYLHAAQRVLLAGRQ
                     VLLMVPEINLTPQLEAALRARFEPIIGAGGLAVLHSGLADGERIESWSRVQRGQARLL
                     LGTRMAVFAPMPELGLIVVDEEHDPSYKQQEGLRYSARDLAVWRAHDLDIPVVLGSAT
                     PSLESWQHAERGHYLRLTLQNRASASSLPAVRLVDTRRLQMKQGLSPQLIDAMGERLA
                     HGEQSLVFLNRRGYAPVLHCGSCGWLSHCPRCSTFTVLHRAEGRSGYRLHCHHCGYQA
                     MVPRACPECGDQDLQPMGRGTQRVEEHLAELFPQARIARIDADSTRKKGSAQALFASV
                     HAGEVDILVGTQMVSKGHDFTRLGLVGVLNADSMLFAHDFRAPERLFAQLMQVAGRAG
                     RHTANGEVIIQTGYPEQPVYQALKRHDYAGFARYMLQEREATGLPPFAHQALLTAEAR
                     ELQQALAFLQRARDVADQAGLPALDAVTRYDPVPLRVVRVAHVERAQLLVESTHRPAL
                     QAFLTAWSAWLPQLAAELRIRWQLEVDPLEI"
     gene            complement(5648..>6193)
                     /locus_tag="VGC69_13910"
     CDS             complement(5648..>6193)
                     /locus_tag="VGC69_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY0296421.1"
                     /translation="DLLHRILGINAQATQQYLNAQIDAGAQAVMLFDSWGGVLADGLY
                     QAFSLAYTRQVVQGLRREHEGRQVPVIVFTKGGGAWLEDIAACGCDAVGLDWTVNLGA
                     ARARTGDAVALQGNLDPMALFGGAQAIRSEARRTLDAFGAMGAGGHVFNLGHGISQYT
                     PPDAVAELVDEVHHYSPALHA"
BASE COUNT         1019 a         2140 c         2046 g          988 t
ORIGIN      
        1 cgggcccggc cgcaggaaga cgaactggtg atcaccacgc tgcaatcgtt cgcgcggcat
       61 aggctgatcg cccggctgcc cggtttccag cgagcccatc cgcattaccg catccgcctg
      121 caaaccggcc tggaactcag cgaactgcag caaggcatgg cggacatcgc catccgcatg
      181 ggccaggggc cttggccggg cctggcgcag caaccgctgc tggaggacga atcgctggtc
      241 gtcgccgcgc cgcactttgc cggcggcaaa ctgccccgca cgccgcggga agtgatccgc
      301 tgcccgctca tcgacgaaac cgacggcacc tggagcgact ggtgccaggc agccggcgtt
      361 cccgagccgg cgcatgccgc ccggatcacc gccaatgact ccaatgtatc cgtgggctgc
      421 gcagaactgg gccatggcct gctgctggcc cggcgcagca tcgtggccgg cctgctggcc
      481 gaaggccgcc tggtacaggt caccaaggtg acgatcccct atccctaccc ctattggctg
      541 gtctggccgc agcgcgacac cctgccggaa aaagtacggc acttcaccga atggatcagc
      601 ggcgagatgg cgcgattcac gcaggaagcc gccaccgaac gctgaacgcc gcgcggcaag
      661 cgtgccggga taccctgcgt acaatacgca acgaatacgc aacgccgatt cacccttgca
      721 ggacactgcc tcatgtctat gaaaaaaacc gaccttgaaa agaacaaggc gctcaaactt
      781 acccactcgc tgaagcaggc gcgctcggac cgcttcggca aaggcgcggc cgacgcgccg
      841 ctcgaccggc gcgaacagcg caagctcgac caggccaaag ggctcgtgcc ctttgcatgc
      901 aagctcaaca gcgaattgat cggcacgctc aatgaacgcg ccgccgcgca cccggaaggc
      961 atgacgggcc tgctcgacga actgctgcgg cacgcgctgg cggcaaagcc gtgacccggc
     1021 ggcgctgaaa tccctgcgcg ccgccccgcg cccgcccggg gccggcgcgg cgcccaaggc
     1081 cttggcctat gacttgttga gcaaggcctt gagcatatcc agccgctgct tggggctcat
     1141 ggccttggtt tcctcgtcct cggcaatgcc cggctgcccc aggcccatct cctcgatgaa
     1201 gcgcgacggc tcgcgcacca cgtcctcgcg cgcgcggcgg cgcttcttgc accagctcag
     1261 gtgcaggctg cgctgggccc gcgtgatgcc cacgtacatc aggcgccgct cttcctggat
     1321 gcgcaccgcc agcgtctcgg cggcctttgc cggatcgccc gtgtcttcgt catccttgcc
     1381 caggtgcggc agcaggcctt cttccacgcc ggccaggtac acgtgcgggt actccaagcc
     1441 cttggaggca tgcagcgtgg acagcttgac cgcgtccggc tcctcgtcgt cgccgcgctc
     1501 gagcatcgtc accagcgcca cgtgctgcac caggtccaag agcgtcatgc cgtcttcctc
     1561 ggccttgcgc ttgagccagc cggtgagctc cagcacgttc tgccagcggt tctgcgccgg
     1621 ccgctcttcc agcgtgtcgt acaggtagcg ctcgtactgg atcgcgccca gcaggtcgtc
     1681 caggatcacg ccggccggtt cggcagattg aatgccgccc ccgcgctgcg ccgtctccgc
     1741 cccgcggccc gcgcgccact ggatgcggcg gacgaattcc gtgaacgtgc gcagcggctc
     1801 cagctggcgc gccgccagct ggctttcgag gcccgactcg gccactgccg ccagcatcga
     1861 cacgccgcgc gaggacgcat actggcccag cgtctgcaag gtgctctggc cgatgccgcg
     1921 cttgggcgtg gtgaccgcgc ggatgaaagc cggatcgtcc tcgtcgttgg cgatcaggcg
     1981 caggtaggcc aggatgtcgc gcacctcggc cttgtcgaaa aagctctgcc cgcccgagat
     2041 cgtgtagggg atcttcacgt tgcgcagcgc ctgctccagg atgcgcgact ggtgattgcc
     2101 gcgatacagg atcgcatagt ccttccattg ggagcggcgc tcgaagcgcg aggccgagat
     2161 ctgcatcgcc acgttctcgg cctcctgctc ctcgccgtcc atggccgaca cggtaatggg
     2221 ctcgccggcg cccaggtccg accacagctt cttctcgaac agcttggggt tgcgcgcgat
     2281 cacgctattg gcagcggcca ggatgcgctg caccgagcgg tagttctgct cgagcttgat
     2341 gagcttgagc tgcgggtagt cggcggtgag cttggccagg ttttccaccg tggcgccgcg
     2401 ccaggcgtag atggcctggt cgtcgtcgcc caccgcggtg aacatggcgc gcatgcccgc
     2461 cagcagctgc accagccggt attggcaggc attggtgtcc tggtattcgt ccaccagcag
     2521 atagcggatg cggttctgcc agcgcgtgcg caccgcttcg tcgcgttcga gcacctcggc
     2581 gggaatgcgg atcaggtcgt cgaaatccac cgcctggtag gcggccagcg tggccgcata
     2641 gctgcggtac acctgcacgg cctcgacctc cgacggtgtc ttggccgcct gcgcggcctg
     2701 gtcgggcgtg atcagctggt tcttccacag cgaaatgatg gactgcacct cgcgcaggcg
     2761 gccgcggtcg gtggtggcca gcaagtcctg gatgatgccc atggcatcgt ccgaatccag
     2821 aatggagaac tgcggcttga gccccgcgtg cgccgcctct tcgcgcagga agcgcacgcc
     2881 cagcgcgtgg aaagtgctga tggtgagccc tttggccagc ttggggtcga ccagggtctt
     2941 gacccgctcg gccatctcgc gcgcggcctt gttggtgaag gtgagcgccg ccacattgcg
     3001 gcccatgtag ccgcactcgc gcagcagata ggcgattttc tgcgtgatca cgcgcgtctt
     3061 gccgctgccc gcgccggcca gcaccaggca ggggccgttc aagtacagca ccgcttcttt
     3121 ctgggccggg ttcaggccgt ggccgatggg cgtatcgctt gcggacatca tatttccagt
     3181 gggtcgactt caagctgcca gcggatgcgc agttcggccg ccaattgcgg cagccaggcg
     3241 gaccaggccg tgagaaaagc ctgcaaggcc gggcgatgcg tgctctccac cagcagctgg
     3301 gcgcgctcca catgggccac gcgcaccacg cgcagcggca ccgggtcgta gcgcgtcacg
     3361 gcgtcgagcg ccggcaggcc ggcctggtca gccacgtcgc gcgcgcgctg cagaaaggcc
     3421 agggcctgct gcaactcgcg cgcctcggcc gtgagcaggg cctggtgggc aaagggcggc
     3481 aggccggtgg cctcgcgctc ctgcagcata taccgcgcaa agcctgcgta gtcgtggcgt
     3541 ttcaaggcct ggtacaccgg ctgctccggg tagccggtct ggatgatgac ctcgccattg
     3601 gccgtatgcc ggccggcgcg gcccgccacc tgcatcagct gcgcgaaaag gcgctcgggc
     3661 gcgcggaagt cgtgggcgaa cagcatcgaa tcggcattga gcacgcccac caggcccagg
     3721 cgcgtgaagt cgtgcccctt ggacaccatc tgcgtgccca ccaggatgtc gacctcgccg
     3781 gcatgcaccg aggcgaacag ggcctgcgcg ctgcctttct tgcgcgtgct gtcggcatcg
     3841 atgcgcgcga tgcgcgcctg cggaaacagc tcggccaggt gttcttccac gcgctgcgtg
     3901 ccgcgcccca tgggctgcag gtcctggtcg ccgcattcgg gacaggcgcg cggcaccatg
     3961 gcctggtagc cgcaatgatg gcagtgcagg cgatagcccg accgcccctc ggcgcgatgc
     4021 agcaccgtga aggtggagca gcgcgggcaa tggctgagcc agccgcacga accgcaatgc
     4081 agcaccggcg catagccgcg ccggttcagg aacaccagcg actgctcgcc atgagccagg
     4141 cgctcgccca tggcgtcgat cagttgcggc gacaggccct gcttcatctg caggcggcgc
     4201 gtgtccacca ggcgcacggc gggcaggctg ctggcgctcg cgcggttctg cagcgtcagg
     4261 cgcaggtagt ggccgcgctc ggcatgctgc caggactcca gcgacggcgt ggccgacccc
     4321 agcaccacgg gaatgtccag gtcgtgcgcg cgccacacgg ccaggtcgcg cgccgaatag
     4381 cgcaggcctt cctgctgctt gtaggaagga tcgtgctctt cgtcgaccac gatcagcccc
     4441 agctcgggca tgggcgcgaa caccgccatg cgcgtgccca gcagcaggcg cgcctggccg
     4501 cgctgcacgc gcgaccagga ctcgatgcgt tcgccgtcgg ccaggccgct gtgcagcacg
     4561 gccaggcccc cggcgccgat gatgggctcg aagcgcgcgc gcaaggcggc ttcgagctgc
     4621 ggggtgagat tgatctcggg caccatcagc agcacctgcc gcccggccag cagcacgcgc
     4681 tgcgccgcat gcaggtacac ctcggtcttg ccgctgcccg tcacgccatg cagcagcacg
     4741 ggcttgaagg cccgcagcgc gtcgatggca tcgacggcgg cccgctgctc cgcatgcagc
     4801 ccgggaggcc gcgacaccgc ggcggcgacg gcatcagagg cgtccgagat atcggcggta
     4861 tcggtggcct tgcccgcgcg cttggactgc gaggcatcgc ggcgcgccac ggggccctgc
     4921 cccgaccgct tgccctggta ggccgacggc ttgcgcaagg gcggcggcag cacgggcagc
     4981 atgacttcgc ccagcggccg ctggtagtac tcggccgcga aactggccag gcgcatccag
     5041 tcggacgaaa aaggcggcag gtcgcgcagc acctgctcca cgggcttgac cagggcaggg
     5101 tcgatggtcg gcgcgtcggt ctgctcgacc accacgccca ccagcttgcg ccgtccgaag
     5161 ggcacgatca cgcgcaggcc gggttcgacg gcttggtcgc tgcgatagtc gaacaggccg
     5221 ggcagcggca cgtcgagcgc cacccgcaac cagctcggcg atggatcgga cggcccggcc
     5281 ggggagggat caggggccgg ctgcgtcatg ccgcctcggg ccctatgtca gcttgtaact
     5341 taaagtgctt tcttgattta tgcgctaaac ccctgctgtt cagagggcag ctcaaaactt
     5401 ttacccagct tgtggataac tttggggaaa accatcgggc cgattcccgg acaaggcggg
     5461 aatatgtgtc tgaaaaatag caaaaaaccg tgtagcgact cgatattcca ttggaaatca
     5521 taaggttata cataaattcc agaatcactc acaagttgcc agggtaattc ccgatttgtt
     5581 tgccgctgtg cacaactccg cctgagaggc acaggtttgc acgcatcggc accgaagcag
     5641 tcactcacta tgcgtgcaag gccgggctgt agtgatgcac ctcgtcgacc agctcggcca
     5701 cggcatcggg cggcgtatat tgggaaatac cgtgccccag gttgaacacg tgcccccccg
     5761 cgcccatggc cccgaaagca tccagggtac gccgggcttc ggaccggatg gcctgcgcgc
     5821 cgccgaacag cgccatcgga tccagattgc cctgcagggc cacggcgtcg ccggtgcggg
     5881 cccgggccgc ccccaggttg accgtccagt ccaggcccac ggcatcgcag ccgcaggcgg
     5941 caatgtcttc gagccaggcg ccgccgccct tggtgaagac gatgaccggc acctggcggc
     6001 cctcatgttc gcggcgcagg ccctgcacca cctggcgcgt ataggccagc gagaacgcct
     6061 ggtacaggcc atcggccagc acgccgcccc agctgtcgaa cagcatcacc gcctgggccc
     6121 cggcatcgat ctgcgcgttg aggtattgct gcgtggcctg ggcattgatg cccagaatgc
     6181 ggtgcagcag atc
//
LOCUS       DASWPO010000265        15103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3097536, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000265 DASWPO010000000
VERSION     DASWPO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15103
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097536"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3359)
                     /locus_tag="VGC69_13915"
     CDS             complement(<1..3359)
                     /locus_tag="VGC69_13915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HEY0296422.1"
                     /translation="MGTERGVGVVNAGAMAAQAGDLTLTASGQLVQSGQMTASGNTSI
                     STAGGVDNSGTVYAQNAASISTGGALTNSGTVAAQQDTTLNAASIASTGVLGAGINSD
                     GTVGTHGTLSVVSAGALDNSGGTLIAPALTIQSGDLANRGGTLSAQSSAVLGVAALDN
                     SAGGRISAQNLAIADTGALDNAGGSVEANGTLNVSAQAISNDGGTLANGGTGTTTVAA
                     SGTLTNTNGGLIGGNGDVSVSAQGTLTNTGGLLYGGTALNLGMTQPGATLVNDGGQIE
                     SVQDISMAVASMSNAGGAVSANRDIAFSGALSGAGAMTAGHDLTLALQGDFTNAAAGT
                     LHADNDLTLGVTGALTNAAQLEAGHALTVNAANVVNAAGAAMNSAATTVNATGGIANA
                     GAIEGDAVATHSTSLDNTGAVIGNNVSATAADISNAGAQAVIAGAASVGVYASHSLTN
                     SDGALLYSGGDMELARDSARDSTGLLANQTGTITNRAATIEAAGNLDVAAHTLNNERT
                     GVQTQAGAPVTTAGPTLTLWTAGLPLVGGDVGYFSDLYPQWSFNTAIGTSVIQQLATP
                     VTVTLSASQVTNLNTANQTFSLAAPLQDQTTYVASQEAAPQIITRTITNNPTQYYRSL
                     AQNPDGTVTITFWPDYDPSKNIAPDQVQLRYDLGPDSHDYVETSRTAAITTTTDQLLN
                     AGTPALMQAQGAIRVNADGGAINNASSTMAAGGDLIRRATGGTVNDTGILLQQTSTET
                     DTSTFYWHAKTSGDTDTQTVVDGTVPLSTATVAALPAIATSNQTVRTDAQNITIGSVN
                     PVGQTVTGAGEPGGSVAQTLGGAGVAIPGLVLPTNALYTYHPAPGSGPLIETDPRFTS
                     YSNFVSSDYMLQQLGLNPQNVEKRLGDGLYEEQLVMGQVTQLTGRTFLGSYTDNLDEY
                     TALMNSGVQYAQQFGLAVGVGLTAEQMSALTTNMVWLVNQTVMLPDGTQQTVLVPQLY
                     LAQSNAVDLQASGALVAGGNVSLNAAGDVANSGQVAGDVATTVIGNNVVNRGVIGNGG
                     TTTVAAVQDVKNLGGSIGGVDTTVVAGNDIVNQSTTARAAVNTGNAGFYSNASGMAIQ
                     SVGMIAGTGSVTLAAGRDVDIDGAAVQSGGDA"
     gene            3699..4904
                     /locus_tag="VGC69_13920"
     CDS             3699..4904
                     /locus_tag="VGC69_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296423.1"
                     /translation="MDNTIGAEEAKKGIAWFCHCYDTYRSAINYRVGAAPSLPQHAVP
                     AWQLLAVSSVFLIISEIFGIACLGQLNTLSLLDPVQISNPSSVLFMLKSTIEEAFDGL
                     VVHHTINKSITIIAFASLVCYFFSWIMAGVIYRSEKGKQGALAHSIDYLYLAAALHIE
                     CTLTWLTLSILALVIGNFALPTGFLLHHRNATGFIFTIGAASVFSIEFLKFFWFRRKA
                     GLPLKSIPSKILAPLFVAAVCPLLLLEITLPADSLFPSSVTITMSPSCSKEGCYAYVR
                     SQYTRPIYLDSTIIIPITDAPLGALSNEKRVGMLEIEIVSLKEKEPYLSEPGEEQLVK
                     ISSATFRCDKPRSLDIGVMRAFGKPVTIGHILNQEPSHSQLTLNTNIAGTLFYLLRNE
                     SLVQKENCT"
     gene            4970..6028
                     /locus_tag="VGC69_13925"
     CDS             4970..6028
                     /locus_tag="VGC69_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0296424.1"
                     /translation="MRTNLNAAFAALLAAALFGATTPVAKSLLGTMSPFMLAGLFYLG
                     SGLGLAVTMAAMRLAKKANPAQASRIQTREVPWLLGAILAGGVAGPVFLMFGLTTTPA
                     VTSSLLLNIEGVFTAVIAWVVFRENVDLHVFLGMVAIVGGGVLLSWQPGASGIAPGAL
                     LVGAACLCWAVDNNLTRKVSTNDAMVIACLKGLVAGAVDLALALLSGSVLPDMGKTAA
                     AMSTGFAGYGISLVLFVVALRNLGTARTGAYFSIAPLFGVILSLALWPELPSPLFWIA
                     AALMALGVWLHLRERHEHPHAHEALAHTHRHRHDDHHRHDHAFEWDGREPHTHPHRHA
                     PMTHAHAHFPDIHHRHDH"
     gene            complement(6061..7131)
                     /locus_tag="VGC69_13930"
     CDS             complement(6061..7131)
                     /locus_tag="VGC69_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296425.1"
                     /translation="MGGQFSSRRRFIKTTGAAGLAAIAAPALWIPAARAASGQLVVAD
                     YGGAPQAAMRKAFYDPFEKATGIQVVSVAHDADPTTAFKVSVDSGSNVWDVCLMTPDH
                     VARLSDPKNYLAPLNIADSDAKGILPGMLASNWFGFSVYSVLMAYRTDKYANGPAGWR
                     DFWDTDKFPGRRGMFRYPGGTMENALLADGVAPKDLYPIDVDRALKSLSKIRKSVSVW
                     WTNGAQNTQILQNGEVDMSDTWNSRVLTAIDAGAPVKMVWEGTYNVDGWAIPVSTSRA
                     DQARQFVRFCIQAERQAEYCNLIANGPSNNDAYKLIDPKRAELLPTYPKNFERLAKHD
                     AAWWGKHYDAVNEKFQEWLLAA"
     gene            7330..8838
                     /locus_tag="VGC69_13935"
     CDS             7330..8838
                     /locus_tag="VGC69_13935"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0296426.1"
                     /translation="MGIRNRQQYLDSLRDDREIHIDGERVKDVTRDPRLSGGAQAIGD
                     LYALQCEPELQDSLSFSLEPGGERFAMSFLEPASVADLQRRGVALKRTADATFGMMGR
                     SPDFMNVGITALASASEVFDHSADGRFFAANVRRHYERARHLDLAATHVQVNPQVDRS
                     KPVHQQAIDIALKVVKETDAGFFVKGARQVATLAQFANDILVMPSVVVNSEPEAADYA
                     LAFALPIATQGLKFVSRPSTIPQNAAHFLDHPLSLRFEEGDAVVIFDDVFVPWENTFV
                     YRDAAMCNALYQRTFLGDHYTHQSMLRAQAKSEFMIGLGCYLAKRTHVDVFPNVQTTL
                     APLLIDLETHKGLVELAVLKAAPTPFGTVAPNRLLLHSAQLFFFERFQKMVEAVRTIA
                     GSGLVGAASYAEFAGEAAQYMTTYFQSEGMDAEYRVRLLRLAADATMSAFSSRQLVYE
                     RYYQGDPVRRAAMFYKNYPKEPLYQIIEDALEDMRHRAEKFAPDGPGGPDQA"
     gene            complement(8872..9663)
                     /locus_tag="VGC69_13940"
     CDS             complement(8872..9663)
                     /locus_tag="VGC69_13940"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0296427.1"
                     /translation="MNAPAKESLDELRPAGMAVGEWRQRLELAACYRLFDWMGWTELI
                     YNHITLRVDDPGASSPHYLINPYGLHYAEVTAGNLIKIDSAGNKLGDSPWPVNPAGFV
                     IHSVIHTARPDAHCVIHTHTTAGMAVACKEAGLRSDNFYSAMLCGDVAYHDFEGITTD
                     PAEQPLLRASLGGCDILVLRNHGMLVIGRSVPDAFQRGWTLERACQVQMASDAMAGPN
                     RAVRPEVLERIPQQRAPMRTGNSKPGQLAFDGMLRRAGIRYADLV"
     gene            complement(9714..10553)
                     /locus_tag="VGC69_13945"
     CDS             complement(9714..10553)
                     /locus_tag="VGC69_13945"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase family protein"
                     /protein_id="HEY0296428.1"
                     /translation="MAELIGGIGASHSPSIAMAYDRRQEADPAWAPFFEAAETLRAQV
                     AQARPDVLVFFYNDHLWHFDLDLVPTFALGVAEQFELAPERPTQRPLPAARGHADLAW
                     HIARSLVAQEFDIATSQRLALDHGFFSVMPLICDPPWPVKVIPVAVNMIQHPLPTPAR
                     CWKLGQAVARAVASYPGSQRVMMVGTGGLSHQLHGPNFGYTNPAWDQAFMDSLVHEPQ
                     SLLALTHDDYMQRGGAESVEMIVWLIMRAALRRPEIRLRAYHGGLLTGLGLLHLAEAG
                     ISG"
     gene            complement(10546..10959)
                     /locus_tag="VGC69_13950"
     CDS             complement(10546..10959)
                     /locus_tag="VGC69_13950"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296429.1"
                     /translation="MNRLMPRAQPSPPGTGLPASRIAGLLVSDGQLLAQAAGLNRLLL
                     AIGRQADAGADFVARPEAWARRYGVPEAQYDLLMARDWNGLLAAGASIYALAKGAKAF
                     GLNLLEIGAAMRGTTGQALLAELPLHDLRDGPAHG"
     gene            complement(10956..11945)
                     /gene="ispH"
                     /locus_tag="VGC69_13955"
                     /gene_synonym="lytB"
     CDS             complement(10956..11945)
                     /gene="ispH"
                     /locus_tag="VGC69_13955"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0296430.1"
                     /translation="MNNAATTAVTQPGAEIVLAQPRGFCAGVDRAIEIVERALELHGA
                     PIYVRHEIVHNRYVVSDLRAKGAVFIEELDEAPAGAIVVFSAHGVAKTVRVEAESRGL
                     LVFDATCPLVTKVHVEVARMHAAGREIVMIGHKGHPEVEGTLGQVKDGMYLVETVEDV
                     AQLQVRDPSNMAYVTQTTLSVDDAATVSAALRARFPLISEPKKSDICYATQNRQDAVK
                     VMAPGCDVVLVVGSVNSSNTNRLREVAERKGAAAYLVDGADSIDPAWLQGSRRVGITA
                     GASAPELLVQQVVTRVKELGAVSVRTLDGLEENVAFPLPKGLSRKSAQIEDLE"
     gene            complement(11945..12421)
                     /locus_tag="VGC69_13960"
     CDS             complement(11945..12421)
                     /locus_tag="VGC69_13960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0296431.1"
                     /translation="MNAPSTEVNNLIRPDSYLTLHYRIVLASGPAEDSVFVDTFDGRP
                     ATLQMGSGLWAPGLEAALIGHAEGERFSVTLPPAAAYGDRNPELIQRVTRAMLAEHAG
                     ADATFDPGDMVEFAAPNGGRYSGILKEINDDWALFDFNHPLAGNALTLEVAILGVL"
     gene            12469..13152
                     /gene="radC"
                     /locus_tag="VGC69_13965"
     CDS             12469..13152
                     /gene="radC"
                     /locus_tag="VGC69_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY0296432.1"
                     /translation="MKLSESLPGHQRPRERLLRHGAAMLNDAELLAVALRTGTRDVNA
                     VELGGQLIQRFGGLRGLFGATPNELREVPGLGDAKACTLAAVLELARRAMEEDLHRHD
                     ALSHPDRVKQYCRVALSHRTVEHCIALYLDNRLNLIATGELAKGTLAQAPVYPREVVR
                     EALRHHAAALILAHNHPSGLAQPSQADLELTRQLRRALALVDVRLVDHLIVAAGQVVS
                     MAQLGMISQ"
     gene            13321..13587
                     /locus_tag="VGC69_13970"
     CDS             13321..13587
                     /locus_tag="VGC69_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEY0296433.1"
                     /translation="MKEGIHPEYREVVFMDVQTGDKFVTRSTINTRETVELDGKTYPL
                     FKCDVTSKSHPFYTGAQTRLVETGRVEKFRARFARTTGTSAAKA"
     gene            13724..>15103
                     /locus_tag="VGC69_13975"
     CDS             13724..>15103
                     /locus_tag="VGC69_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0296434.1"
                     /translation="MPPIMRSTPARLTETGTTKLPRLALFGLAFAYIVAGLFMRDPWK
                     TDDVVGLATMITALHEGTWLLPQIGHLAYAQEGPLLTWVGAVAIKLFGPFLGEFTAGR
                     LPNLLWFAMSAVSVWYGTYLVGRRAEAQPLALPFGGEPPPRAYGRMLADAALLLLLAT
                     VGILQRMHETSVLPAVMAWQALVFYALTRMPEKPITGALTLGLAIAGSFLTRGWLGGL
                     PVMVGTLVAFIPPGDLRARLRWLPLALLVAAGLILAWWIPATHASDYWVRNWKAWNLG
                     NYDWPAFNRSGRALRDLTWFLWPTWPLALVAVWRWRAWLFSTHIWPALSLAACALLQL
                     FFVSDPDDGVYIMLAVPCASLAALSLPTLRRGVINTLDWFAVMCFSLTAITAWLAWVA
                     VLFGMPAQISRNIARHTAGYVPPISWLALLLAAAITVAWIALIVWRLRVRPTALWRGT
                     VLSASGLAAT"
BASE COUNT         2476 a         4983 c         4827 g         2817 t
ORIGIN      
        1 gcatcgccgc cggactgaac cgccgcgccg tcgatatcca cgtcccggcc tgctgccagc
       61 gtcacgctgc cggtccccgc gatcatgccg accgactgta tcgccatgcc gctcgcattc
      121 gaatagaacc ccgcattgcc ggtgttcacg gcggcccgcg cggtcgtact ctggttgacg
      181 atgtcgttgc ccgccacgac tgtcgtatcg acgccgccga tactgccgcc gaggtttttc
      241 acatcctgca ccgcggctac cgtcgtcgta ccgccgttgc cgatcacgcc gcggttgacc
      301 acgttgttgc cgatcacggt cgtcgccacg tcgcccgcca cctggccgct attggccacg
      361 tcgcccgccg cgttcaggct gacgttgcca cccgcgacga gcgcgccgct ggcctggaga
      421 tccaccgcat tggattgggc caggtagagc tgcggcacca gcacggtctg ctgcgtgccg
      481 tccggcagca tgacggtctg gttgaccagc cacaccatgt tggtcgtgag tgccgacatc
      541 tgctcggcgg tcaggcccac gcccacggcc aggccgaact gctgcgcata ctggacgccg
      601 ctgttcatca acgccgtgta ttcgtccagg ttgtccgtgt acgagccgag gaaggtgcgc
      661 cccgtgagct gcgtgacctg gcccatcacg agctgctctt cgtagagccc gtctcccagg
      721 cgcttttcca cgttctgcgg gttcaggccc aactgctgca gcatatagtc gctgctgacg
      781 aagttcgaat agctcgtgaa gcgcggatcg gtctcgatca gggggcccga acccggcgcg
      841 gggtgatagg tgtagagcgc attggtcggc aggaccaggc ccggaatggc cacgcccgcg
      901 ccgccgagcg tctgcgcgac gctgccgccc ggctcgccgg cacccgtcac tgtctggccg
      961 acggggttca ccgagccgat ggtgatgttc tgcgcatcgg tccggaccgt ctggttggac
     1021 gtggcaatgg ccggcagggc ggcgacggtc gcggtcgaca gcggaaccgt cccgtccacg
     1081 acggtctggg tatcggtatc gccgcttgtc ttggcatgcc agtagaaggt cgaggtatcc
     1141 gtctccgtgc tggtctgctg cagcaggatg cccgtatcgt tgaccgtgcc gcccgtcgcg
     1201 cgccggatca ggtcgccgcc ggccgccatc gtggaggagg cgttgttgat ggcgccgccg
     1261 tcggcattga cgcggatcgc accctgcgcc tgcatcagcg cgggcgtgcc tgcgttcaaa
     1321 agctggtcgg tcgtggtcgt gatggcggcc gtgcggctcg tttccacgta gtcgtggctg
     1381 tccggcccca ggtcgtaacg cagttgcacc tggtccggcg cgatgttctt cgaggggtca
     1441 tagtccggcc agaacgtgat cgtcaccgtc ccgtccgggt tttgcgccag gctccggtaa
     1501 tactgcgtcg ggttgttggt gatggtccgg gtgatgattt gaggggcggc ctcctgggag
     1561 gcgacgtacg tggtctgatc ctgcagcggg gcagccaggg aaaacgtctg gttggcggtg
     1621 ttgaggtttg tcacctgcga ggcagacagc gtcaccgtca cgggcgtggc caattgctga
     1681 atgacggagg tgccaatggc ggtattgaaa ctccactggg ggtagagatc gctgaaatag
     1741 ccgacatctc cgccgaccag gggcaggccc gccgtccaca gggtcagagt tgggccggcg
     1801 gtggtcacgg gcgcaccggc ttgggtttgc acgccggtac gttcgttgtt cagcgtatgc
     1861 gcggccacgt ccaggttgcc tgccgcctcg atggtcgcgg cgcggttggt gatcgtgccg
     1921 gtctggttcg caagcaggcc cgtcgaatca cgcgcgctgt cgcgcgccag ttccatatcg
     1981 ccgccgctgt agaggagcgc gccgtccgaa ttggtcaggg aatgggaggc atacacgccc
     2041 accgacgcgg ccccggcaat gaccgcctgg gcaccggcat tcgagatatc cgccgccgtc
     2101 gcgctgacgt tgttgccgat caccgcgccg gtattgtcca ggctcgtgct gtgcgtcgcg
     2161 acggcatcgc cttcgatggc gcccgcgttc gcgatgccgc ccgtggcgtt gacagtggtg
     2221 gccgctgaat tcatggccgc gcccgcggcg ttgacgacat tggccgcatt gaccgtgagg
     2281 gcatggcccg cttccaactg cgcggcgttg gtgagcgcgc ccgtcacgcc cagcgtcagg
     2341 tcgttgtccg catgcagggt gccggccgcg gcgttggtga aatcgccttg gagcgcgagc
     2401 gtcaggtcat gtcccgccgt catcgcgcct gcgcccgaca aggcaccgct gaaggcaata
     2461 tcacggttgg cgctcaccgc cccgcccgcg ttggacatcg atgccacggc catcgatatg
     2521 tcctggacgc tctcgatctg gccgccgtcg ttgacgagcg tcgcgcccgg ctgggtcatg
     2581 cccagattca gcgccgtgcc accataaagc aggccgcccg tattcgtgag cgtaccttgc
     2641 gcagataccg agacatcgcc attgccgccg atcaagccgc cattcgtgtt ggtcagcgtg
     2701 cccgatgcgg caaccgtggt tgtgcccgtg ccgccgttgg cgagcgttcc gccgtcattg
     2761 gagatggcct gcgcgctgac attcagcgtg ccattcgctt cgacagatcc acccgcattg
     2821 tcgagcgcgc ccgtatccgc gatagccaga ttctgggcgc tgatccgtcc gcccgcactg
     2881 ttgtcaagcg cagccacgcc gagcacggcc gacgactggg cggaaagcgt accgccccgg
     2941 tttgcaagat cgcccgactg gatcgtcagc gcgggagcaa tcagggtgcc gccgctgttg
     3001 tcgagcgcac ccgccgagac gaccgaaagc gtgccatgtg tgccgaccgt cccgtcgcta
     3061 ttgatacccg caccgagcac gccggtcgag gcgatgctgg cggcgttgag cgtggtgtct
     3121 tgttgcgcgg cgaccgtgcc gctatttgtc agtgcgcccc ccgtgcttat cgaggccgcg
     3181 ttctgggcat agaccgtgcc gctattgtcg accccgcctg ccgtgctgat acttgtattg
     3241 ccactggcag tcatctggcc ggactgcaca agctggcccg aggccgtcaa ggtcaggtcg
     3301 cccgcctgcg cggccatggc gccggcgtta accacgccta ccccgcgctc ggtgcccacg
     3361 agcgtgcatt gcgcttttat tttggcaatg ccggttattt attggcttgc acgaacaggt
     3421 ctaccctgcg ccaatattgc cgcggagctt tctgacacag cggattctaa gaccgagagc
     3481 aatcatgtct cgatgcagat cagtttgatt ttccttcttt gacatttctt gattcaagaa
     3541 aagcaaattc agctcttgaa aatcaagcag tacaaaccaa tcatcgcatc ggatggcaaa
     3601 tcgatgcttg agttcgacat ctcagccgat tagttaagac catttctttg tctgcaccat
     3661 cgcatcttta tgaatgtgat ttgactttgg agaaacggat ggacaacacg attggcgctg
     3721 aagaagccaa aaagggtatc gcctggtttt gtcattgcta tgacacttac cgctcggcca
     3781 taaattacag agtcggcgca gcgccatcac tcccccagca tgcagttccc gcttggcaac
     3841 tgctagctgt ttcatctgtt tttcttatca tttctgaaat atttggcatt gcctgtttag
     3901 ggcaattaaa cacattatcc ttgctggatc cagtacaaat ctccaaccca tcgtcagttt
     3961 tatttatgtt gaaatcgacg atagaggaag ctttcgatgg cctcgtcgtt caccatacga
     4021 taaacaaaag cataaccatc atcgcttttg cctctctagt ctgttatttt ttttcatgga
     4081 taatggcagg agtgatctac agatcagaaa aaggcaaaca aggtgcattg gcgcacagca
     4141 ttgactatct ttacttggca gccgcgttgc acattgaatg cacgctcaca tggctaacac
     4201 tatctatcct tgccttggtc atcggaaatt ttgcattgcc gactggcttt cttcttcacc
     4261 atcgcaacgc aactggattt atatttacca tcggagctgc ttctgtattt tcaatagaat
     4321 ttttgaaatt tttttggttc cgccgaaaag ctggtttgcc actcaaaagc attccttcaa
     4381 aaattctggc tcctttattc gtggcggctg tttgccctct gttgctattg gaaataacgt
     4441 tacctgccga ctctctattt ccatcgtccg taaccatcac aatgtctcct tcgtgcagca
     4501 aggaaggctg ctatgcatac gtccgctccc agtacacgcg tccaatttat ttggacagca
     4561 caatcattat ccctattaca gatgcacctc taggcgccct ctcaaacgag aagagagttg
     4621 ggatgcttga aatcgagatt gtttccctca aagagaaaga accatatctc tcggagcctg
     4681 gagaagaaca attggtcaaa atttcgagtg ctacatttcg atgtgataag cctcgaagcc
     4741 tggatattgg ggtgatgcgc gcatttggta agccagttac catcgggcat attctcaatc
     4801 aagaacccag ccatagccaa ttgactctta ataccaatat tgccggaaca ttgttctatc
     4861 tcttgcgcaa tgaatcattg gtacaaaaag aaaattgcac ttaacgagaa tgatctcggg
     4921 agtacgcttg gggcacgaca aacccccata cctccttttc cagcccgcca tgcgcaccaa
     4981 tctaaacgcg gcgttcgcgg cactgctggc cgcggcgctg tttggcgcca cgacgcccgt
     5041 cgccaagagc ctgctcggaa ccatgtcgcc attcatgctg gccgggctct tctacctggg
     5101 cagcggcctc gggctggcgg tgacgatggc cgcgatgcgc ctggccaaga aggccaaccc
     5161 cgcgcaggca agccgcatcc agacacggga ggttccgtgg ctgctcgggg ccatcctggc
     5221 cgggggtgtc gcaggccccg tcttcctgat gttcggcctg accacgacgc ccgcggtcac
     5281 cagttccttg ttgctcaaca tcgagggcgt gtttaccgcc gtgatcgcct gggtggtgtt
     5341 tcgagaaaat gtggacttgc acgtctttct cggcatggtg gccattgtcg gcggcggcgt
     5401 gttgctatcc tggcagcccg gagcgtccgg cattgccccg ggcgcgcttc tcgtgggggc
     5461 ggcctgcctc tgctgggccg tcgacaacaa cctgactcgc aaggtctcga caaacgacgc
     5521 gatggtcatc gcctgcctaa aggggctggt ggctggcgcg gtcgacctgg ccttggcgct
     5581 gctatccgga tcagtgctgc cggacatggg gaagacagca gccgcgatgt cgacgggctt
     5641 tgccggctac ggaatcagcc tggtgctgtt cgtcgtcgcg ctgcgcaacc tgggaaccgc
     5701 ccggacgggg gcgtatttct ccatcgcgcc cctgttcggg gtcatcctgt cgctcgcgct
     5761 gtggcccgag cttccttcgc cgctattctg gatcgccgcg gcgctgatgg ccctcggggt
     5821 gtggctgcac ctgcgcgagc ggcacgagca tccgcacgct cacgaggcac tcgcgcacac
     5881 ccaccggcat cggcacgacg accatcaccg gcacgaccac gccttcgagt gggatggccg
     5941 ggagccgcac acgcaccctc accggcatgc gccgatgacg cacgcgcatg cgcacttccc
     6001 cgacatccac catcgccatg accactaagc tgccgggctg cgccgggcag catttgccct
     6061 ttacgccgcc agcagccact cctggaactt ctcgttgacg gcgtcgtagt gcttgcccca
     6121 ccaggccgcg tcgtgcttgg ccaggcgttc gaagttcttg ggataagtcg gcagcagctc
     6181 ggcgcgcttg gggtcgatga gcttgtaggc gtcgttgttg ctggggccgt tggcgatgag
     6241 gttgcaatac tcggcctggc gttcggcctg gatgcagaag cgcacgaact ggcgggcctg
     6301 gtcggcgcgc gaggtgctga cgggaatggc ccagccgtcg acgttgtagg tgccttccca
     6361 gaccatcttc accggcgcgc cggcatcgat ggcggtcagc acgcgggagt tccaggtgtc
     6421 ggacatgtcg acttcgccgt tttgcaggat ctgcgtgttc tgcgcgccgt tggtccacca
     6481 caccgacacc gacttgcgga tcttggagag cgacttcagc gcgcggtcca cgtcgatggg
     6541 atagaggtcc ttgggcgcca cgccgtcggc cagcagcgcg ttttccatgg tgccgccggg
     6601 gtaccggaac atgccgcggc gcccggggaa cttgtcggtg tcccagaaat cgcgccagcc
     6661 cgccgggccg ttggcgtact tgtcggtgcg gtaggccatc agcacgctgt acaccgaaaa
     6721 gccgaaccag ttgctggcga gcatgccggg caagatgccc ttggcgtcgc tgtcggcgat
     6781 gttcagcggc gccaggtagt tcttggggtc ggacagccgg gccacgtggt cgggcgtcat
     6841 caggcagacg tcccagacgt tggagccgga atcgaccgac accttgaaag ccgtggtggg
     6901 atcggcgtcg tgcgccacgc tcacgacctg gatgccggtg gctttttcga agggatcgta
     6961 gaaggccttg cgcatggccg cctgcggcgc gccgccataa tcggccacga cgagctggcc
     7021 ggacgcggcg cgcgcggccg ggatccacaa cgcaggggcg gcaatggccg ccaagccggc
     7081 ggctccggtg gtcttgatga aacgccgacg actgctgaat tgcccgccca tacgctctcc
     7141 tggaatcagt gtgtgaaatg aggcttggat gtctatgcag ggtaaagggg gtacgaggta
     7201 aaaaccaaga tcacgttttc ccaggtatgc atcaacatac attgtttgag atcatcggcc
     7261 ataccccttg cgtcgacgat tcgtgtctgg acacggacgc atcgggatat tctgaccaga
     7321 ggaagaagca tggggatacg caaccgccag caatacctgg attcgctgcg ggacgaccgg
     7381 gaaatccata tcgacggcga acgcgtcaag gacgtcaccc gcgatccgcg cttgagcggc
     7441 ggcgcccagg ccatcggcga cctgtatgcg ctgcaatgcg agcccgaact gcaggacagc
     7501 ttgtcgttca gcctggaacc cggcggcgag cggttcgcca tgtccttcct ggaacccgcc
     7561 agcgtggccg acctgcagcg ccgcggcgtc gcgctcaagc gcaccgccga cgccaccttc
     7621 ggcatgatgg gccgcagccc cgacttcatg aacgtgggca tcactgccct ggctagcgcc
     7681 agcgaggtct tcgaccacag cgccgacggg cggttcttcg cggccaatgt gcggcgccac
     7741 tacgagcgcg cgcgccacct ggacctggcc gccacccatg tgcaggtcaa tccgcaggtg
     7801 gaccgcagca agccggtgca ccagcaagcc atcgacatcg ccctgaaggt ggtgaaggaa
     7861 acggacgcgg gcttttttgt gaagggcgcg cgccaggtgg ccaccctggc gcagttcgcc
     7921 aacgacatcc tcgtcatgcc ctcggtggtg gtcaacagcg agccggaggc ggccgactat
     7981 gccctggcct tcgcgctgcc catcgccacc caaggcctga aattcgtcag ccgtcccagc
     8041 accatcccgc agaacgcggc gcacttcctg gaccacccgc tgtcgctgcg cttcgaggaa
     8101 ggcgacgcgg tcgtcatttt cgacgacgtg ttcgtgccct gggaaaacac cttcgtctac
     8161 cgcgacgcgg cgatgtgcaa tgcgctctac cagcgcacct tcctcggcga ccattacacg
     8221 caccagtcca tgctgcgcgc ccaggccaag tccgagttca tgatcgggct gggatgctac
     8281 ctggccaagc gcacgcacgt ggatgtgttc cccaatgtgc agaccacgct tgcgcccctg
     8341 ctgatcgatc tggagacgca caagggcctg gtggaactgg ccgtgctcaa ggccgcgccc
     8401 acgcccttcg gcacggtggc gcccaaccgg ctgctgctgc attcggcgca gctgttcttc
     8461 ttcgagcgtt tccagaaaat ggtcgaggcc gtgcggacca tcgccggcag cggcctggtg
     8521 ggcgcggcat cctatgccga attcgcgggc gaggcggcgc agtacatgac gacctacttc
     8581 cagagcgaag gcatggacgc ggagtaccgc gtccgcctgc tgcgcctggc ggccgacgcc
     8641 accatgtcgg ccttctccag ccggcagctg gtctacgagc gctactacca gggcgacccg
     8701 gtccggcgcg cggccatgtt ctacaagaac tatcccaagg agccgctcta ccagatcatc
     8761 gaggatgcgc tcgaagacat gcggcaccgg gcggagaagt ttgcgccgga cggccccggc
     8821 ggcccggacc aggcctgata tccgggcctg cgccgcgggc ccggcctccc cttacaccag
     8881 gtccgcgtaa cggatcccgg cgcggcgcag catgccgtcg aaggcaagct gccccggctt
     8941 ggaattgccg gtgcgcatgg gggcccgctg ctgcgggatg cgttcgagca cctccgggcg
     9001 caccgcccgg ttcgggccgg ccatggcgtc ggacgccatc tgcacctggc aggcgcgctc
     9061 cagcgtccag ccgcgctgga aggcatccgg cacgctgcgc ccgatgacca gcatgccgtg
     9121 gttgcgcagc accaggatgt cgcagccgcc caggctggcc cgcaggagcg gctgctcggc
     9181 cgggtcggtg gtgatgcctt cgaagtcgtg ataggccacg tcgccgcaca gcatggcgct
     9241 gtagaaattg tcgctgcgca gccccgcctc cttgcacgcc accgccatgc ccgccgtggt
     9301 gtgcgtgtgg atgacgcaat gggcgtcggg ccgcgcggtg tggatcacgc tgtggatcac
     9361 gaagcccgcc gggttgaccg gccagggcga gtcgccgagc ttgttgcccg cgctgtcgat
     9421 cttgatcagg ttgccggccg tgacctcggc gtagtgcagc ccgtaggggt tgatcaggta
     9481 atgcggcgat gacgcgcccg gatcgtccac gcgcaaggtg atgtggttgt agatgagctc
     9541 ggtccagccc atccagtcga acaagcggta gcaggccgcc agctccaggc gctggcgcca
     9601 ctcgcccacg gccatgccgg ccggccgcag ctcatccagt gattctttcg caggtgcgtt
     9661 catgggtcgt tttccgtcgt gtcgggccgt ccgccagggg gactgtgagg gagtcagccg
     9721 gatatgccgg cctcggccag gtgcagcagg cccaggcccg tgagcagccc gccgtggtag
     9781 gcccgcagcc ggatctcggg ccggcgcaag gcggcccgca tgatgagcca cacgatcatc
     9841 tccaccgact cggcgccgcc gcgctgcata tagtcgtcgt gcgtcagggc cagcagggac
     9901 tgcggctcat gcaccaggct gtccatgaag gcctggtccc aggccggatt ggtgtagccg
     9961 aaattcgggc cgtgcagttg gtgggacagg ccgcccgtgc cgaccatcat cacgcgctgc
    10021 gagcccggat agctggccac ggcccgcgcc accgcctggc cgagcttcca gcatcgggcg
    10081 ggcgtgggca agggatgctg gatcatgttg accgccaccg ggatcacctt caccggccag
    10141 ggcggatcgc agatcagcgg catcacggaa aagaagccgt ggtcgagcgc caggcgctgg
    10201 ctggtcgcga tgtcgaactc ctgcgccacc aggctgcgcg cgatgtgcca ggccaggtcc
    10261 gcgtgtcccc gcgccgcggg cagcgggcgc tgcgtggggc gctccggcgc cagctcgaac
    10321 tgctcggcca cgcccagggc gaaggtcgga acgagatcca ggtcgaaatg ccagaggtgg
    10381 tcgttgtaga aaaagaccag gacgtccggc cgcgcctggg ccacctgcgc gcgcagggtc
    10441 tcggccgcct cgaaaaaagg cgcccaggcg ggatccgcct cctggcgccg gtcgtaggcc
    10501 atggcgatgg agggggaatg cgaagcgccg atcccgccga tcaactcagc catgggccgg
    10561 cccatcgcgc aggtcgtgca gcggcaattc ggcaagcagg gcttgtcccg tggtgccccg
    10621 catggcggcc ccgatctcca gcaggttcaa gccgaaggcc ttggcgccct tggccaaggc
    10681 gtagatgctg gcgcccgcgg caagcaggcc gttccagtcg cgcgccatga gcaggtcgta
    10741 ttgcgcctcg ggcacaccgt agcgccgcgc ccaggcttcg ggccgggcca cgaagtcggc
    10801 gccggcgtcg gcctgcctgc cgatcgccag cagcagccgg ttcagccccg ccgcctgcgc
    10861 cagcaactgg ccgtccgaga ccagcagccc cgcgatccgg gaagctggca ggccggtccc
    10921 cggaggggat ggctgcgcgc gcggcatgag acggttcact cgaggtcttc gatctgcgcc
    10981 gacttgcgcg acaggccctt gggcagcggg aaggccacgt tttcttccag gccgtccagg
    11041 gtgcgcaccg acacggcgcc cagttccttg acgcgggtca cgacctgctg caccagcagt
    11101 tcgggcgccg acgcgccagc cgtgatgcct acgcggcggc tgccctgcag ccaggccgga
    11161 tcgatggaat cggcgccgtc gaccaggtag gccgcggccc ccttgcgttc ggccacttcg
    11221 cgcaggcgat tggtgttgga gctgttgacg ctgcccacca ccagcaccac gtcgcagccc
    11281 ggcgccatga ccttcacggc gtcctggcgg ttctgcgtgg catagcagat gtcgcttttc
    11341 ttgggctcgg agatcagcgg aaagcgcgcc ctgagcgcgg cggacaccgt ggcggcatcg
    11401 tcgaccgaca gcgtggtctg cgtgacgtag gccatgttgg acggatcgcg cacctgcagc
    11461 tgcgccacgt cctcgaccgt ctcgaccagg tacatgccgt ccttgacctg gccgagcgtg
    11521 ccctcgacct cggggtggcc cttgtggccg atcatgacga tctcgcggcc cgcggcgtgc
    11581 atgcgcgcca cctcgacgtg caccttggtg accagcgggc aggtggcgtc gaacacgagc
    11641 aggccccggc tttcggcctc gacgcgcacg gtcttggcta cgccgtgcgc cgaaaacacc
    11701 acgatggccc cggcgggcgc ctcgtccagt tcctcgatga acaccgcccc cttggcgcgc
    11761 aggtcggaca ccacgtagcg gttgtgcacg atttcgtggc gcacatagat gggcgcgccg
    11821 tgcagttcga gggcgcgctc gacgatctcg atggcgcggt ccacgccggc gcagaagccg
    11881 cgcggctggg ccagcacgat ttcggcaccg ggctgcgtga cggcagtcgt agcggcgttg
    11941 ttcatcacag cactcccagg atggccactt cgagggtcag ggcgttgccg gccagtggat
    12001 ggttgaaatc gaacagcgcc cagtcgtcgt tgatttcttt gaggatgccc gaatagcggc
    12061 cgccgttggg cgccgcgaac tcgaccatgt cgcccggatc gaaggtggcg tcggcgccgg
    12121 catgctcggc cagcatggcg cgggtgacgc gctggatgag ctcgggattg cgatcgccgt
    12181 aggcggcggc cggcggcagc gtgacgctga agcgctcgcc ttcggcatgg ccgatcagcg
    12241 cggcttccag gccgggagcc cacaggccgc tgcccatctg caaggtggcg gggcggccgt
    12301 cgaaggtgtc gacgaagacc gagtcctcgg ccgggcccga ggccagcacg atgcggtaat
    12361 gcagcgtcag gtaggaatcc ggacggatca aattgttcac ttcggtgcta ggggcgttca
    12421 agttctgcca ccctgatcaa tgttcgtaaa acctggattt taaagtacat gaagctgtct
    12481 gaaagcctcc ccggccacca gcgcccccgc gagcggctgc tgcgccacgg cgccgccatg
    12541 ctcaacgacg cggaattgct ggcggtggcc ctgcgcaccg gcacccggga cgtcaatgcc
    12601 gtggagctgg gcggccagct gatacaacgt ttcggcgggc tgcgcggcct gttcggcgcc
    12661 acgcccaacg aactgcgcga ggtgccgggg ctgggcgacg ccaaggcctg caccctggcg
    12721 gccgtgctgg agctcgcgcg ccgcgccatg gaggaagacc tgcaccgcca tgatgcgctc
    12781 tcgcacccgg accgggtcaa gcaatattgc cgcgtcgcgc tcagccatcg cacggtggag
    12841 cactgcatcg ccctctacct ggacaaccgc ctcaacctga tcgccaccgg cgaactggcc
    12901 aagggcaccc tggcccaggc ccccgtctac ccccgggagg tcgtccggga ggccctgcgc
    12961 caccatgcgg ccgcgctcat cctggcgcac aaccacccct cgggcctggc ccagcccagc
    13021 caggccgacc tggagcttac ccggcaattg cgccgggcgc tggccctggt ggatgtgcga
    13081 ttggtcgacc acctgatcgt cgcggccgga caggtcgtat ccatggcgca actggggatg
    13141 atttcccaat aaaaaccggg acttgcactc tttcctcaag ccggggtaga attatcgatt
    13201 ccgtttttcc ggatacgtgg tcgaggaaaa gtggctggtg gggtttgaat gcgaacagct
    13261 ttgaacccgg acttccagcc cgactcgatt ggcgacccta accctagcca ggaaatcccc
    13321 atgaaagaag gcatacaccc cgaataccgc gaagtggtgt tcatggacgt gcaaacgggc
    13381 gacaagttcg tgacgcgttc gaccatcaac acccgcgaaa ccgtcgaact ggacggcaag
    13441 acctacccgc tgttcaagtg cgatgtgact tccaagtcgc accccttcta caccggcgcc
    13501 cagacccgcc tcgtcgaaac cggccgcgtc gagaagttcc gcgcccgttt cgcccgcacc
    13561 accggcacca gcgctgccaa ggcctgatcg cttagcgtat aggcccatgc agcggcccgt
    13621 gccgttcatg cagcgtagcg tcgtgacctg ctgcaacaca aaaaagcagc ccgcatgggc
    13681 tgcttttttt tcccggtgag acgataagcg agacgatagc cccgtgcctc cgatcatgcg
    13741 ctccacgccc gcccgtctga ccgagacggg caccacgaaa ttgccacggc tcgccctgtt
    13801 cgggctggcg tttgcctaca tcgtggcggg gctcttcatg cgggacccct ggaagaccga
    13861 cgacgtggtg ggcctggcca ccatgatcac ggcactgcac gaaggcacgt ggctgctgcc
    13921 gcagataggc cacctggcct atgcccagga agggccgctg ctcacctggg tgggcgccgt
    13981 cgcgatcaag ctgttcggcc cgttcctggg cgaattcacg gccggccggc tgcccaacct
    14041 gctgtggttc gccatgagcg cggtcagcgt ctggtacggc acttacctgg tcggccggcg
    14101 cgccgaggcc cagccgctgg ccctgccctt cggcggcgaa ccgccgccgc gcgcctacgg
    14161 ccgcatgctg gccgacgcgg ccctgctgct cctgctggcc accgtcggca tcctgcagcg
    14221 catgcacgag acctcggtgc tgcccgccgt catggcctgg caggcgctgg tgttctacgc
    14281 cctgacccgc atgccggaga agcccatcac gggcgccctc accctgggcc ttgcgattgc
    14341 cggcagcttt ctcacgcgcg gctggctggg cggactgccc gtcatggtgg gcacgctcgt
    14401 ggccttcatc ccgcccggcg atctgcgcgc gcgcctgcgc tggctgcccc tggccctgct
    14461 ggtggccgcg gggctcatcc tggcctggtg gatccccgcc acgcacgcga gcgactactg
    14521 ggtccgcaac tggaaagcct ggaacctcgg caactacgac tggcccgcgt tcaaccgcag
    14581 cggccgcgcc ctgcgcgacc tgacctggtt cctgtggccg acctggccgc tggcgctggt
    14641 ggccgtctgg cgctggcgcg catggctgtt ttccacgcac atctggccgg cgctctcgct
    14701 ggcggcctgc gcgctgctgc agctcttctt cgtgtccgac ccggacgacg gcgtctacat
    14761 catgctggcc gtgccttgcg cttcgctggc cgcgctatcg ctgcccacgc tgcgccgcgg
    14821 cgtgatcaac acgctggact ggttcgccgt gatgtgcttt tcgctgacgg cgatcacggc
    14881 ctggctggcc tgggtggccg tgctcttcgg catgcccgcc cagatctccc gcaacatcgc
    14941 ccgccacacg gccggctacg tgccgccgat atcctggctt gccctgctgc tcgcggcggc
    15001 catcaccgtg gcctggatcg cactcatcgt ctggcgcctg cgcgtgcgcc ccaccgcgtt
    15061 gtggcgcggc acggtgcttt cggccagcgg cctggccgcc acc
//
LOCUS       DASWPO010000266         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2119200, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000266 DASWPO010000000
VERSION     DASWPO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119200"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VGC69_13980"
     CDS             complement(<1..651)
                     /locus_tag="VGC69_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296435.1"
                     /translation="MPRILSGDLPRSRVLAVLLLLVAIVLVFAPFIFPGPRALAVAGK
                     VLVFIVLVASYDLLLGYTGIVSFAHTMFFGIGAYGAAIASARMEPGWTAIALGTGAAL
                     LVSLVLAFVIGLVSLRVRAIFYAMITLAIAAAFQTLVSQLSDLTGGEDGLSFQVPHLL
                     RPAFRLFPGRVLGVAMDGRIVGYYLVFLISLVLFLALLRIVNSPFGRVLQAIRENAF"
     gene            complement(683..1636)
                     /locus_tag="VGC69_13985"
     CDS             complement(683..1636)
                     /locus_tag="VGC69_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296436.1"
                     /translation="MLLVAGLAIVALPLVGDFSTWLTLTIAGLAMGMIVFIVASGMTL
                     VFGLMDVLNFGNGLFITLGAYVACTVLGGMAGWTQSDNLLANLGAVLPAAVIGMLVAG
                     AVGAAFERLIVRPVYGQHLKQILITVGGMIVGEELIKAIWGPQTLPLPLPLALRGAVL
                     MGDAAVEKFRLLALVIGVLVLVLMLWLLNRTKLGLLVRAGVENREMVESLGYRIRHLF
                     IGVFIAGAMLAGLGGVLWGMYQQAVIPDLGAQVNVLIFIVIMIGGLGSTLGCLIGSLL
                     VGLMANYTGFLVPKAALFSNIALLIVILLWRPQGVYPVASR"
     gene            complement(1699..2457)
                     /locus_tag="VGC69_13990"
     CDS             complement(1699..2457)
                     /locus_tag="VGC69_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296437.1"
                     /translation="MSQSAAPLLELRDVHTHIGAYHILHGVDLAVPAGQVTMLLGRNG
                     AGKTTTLRTIMGLWRASSGELRLQGRVIGSAGQHIAPPDMVDQGVAYVPESMGIFSDL
                     SVQENILLAARKARKASELDTARLEWIFGFFPALRKFWLYPAGKLSGGQKQMLAVARA
                     IIEPRRLLLVDEPSKGLAPAIVQNMIEAFAELKRDATTILLVEQNFDFARRLGDQAAV
                     MDNGRVVHYGGMAELSQDQALQTRLLGLSLDSHQ"
     gene            complement(2454..>2596)
                     /locus_tag="VGC69_13995"
     CDS             complement(2454..>2596)
                     /locus_tag="VGC69_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296438.1"
                     /translation="VVRELADRIVVLHNGRLMADGNPAEVIASDIVQQAYLGTAPERE
                     TA"
BASE COUNT          472 a          825 c          940 g          359 t
ORIGIN      
        1 gaaggcgttt tcgcgtatgg cctgcagcac ccgtccgaag ggggagttga cgatgcgcag
       61 caaggccagg aacaggacga ggctgatcag gaacacgagg tagtagccga cgatgcggcc
      121 gtccatggcg acgccgagca cccgcccggg aaacagccgg aatgcggggc gcagcagatg
      181 cggcacctgg aagctcaggc cgtcctcgcc gccggtcagg tccgagagct gcgacaccag
      241 cgtctggaac gccgccgcga tggccagcgt gatcatggcg tagaagatcg cgcgcacccg
      301 caggctgacc aggccgatga cgaaggccag caccagggag accagcagcg cggcgccggt
      361 gcccagcgca atggcggtcc agccgggttc catgcgggcg ctggcgatgg ccgcgccgta
      421 cgcgccgatg ccgaagaaca tggtgtgcgc gaagctgacg atgcctgtat agcccagcag
      481 caggtcgtag ctggccacca gcacgatgaa caccagcacc ttgcccgcca cggccagcgc
      541 gcgcgggccg gggaagatga agggtgcgaa taccagcacg atcgcgacca gcagcaggag
      601 cacggccagg acgcggctgc ggggtagatc gccggagagt atgcgaggca tgccggtgtg
      661 tgtctcgagg aggatgggcc ggtcagcgcg aggcgaccgg atagacgcct tgtggccgcc
      721 acagcaggat gacgatcagc agggcaatat tggaaaagag cgcggccttg ggcaccagga
      781 agcccgtgta attggccatc aggcccacca gcagcgagcc gatcaggcag cccagggtcg
      841 agcccaggcc gcctatcatg atcacgatga agatcagcac attgacctgg gcgcccaggt
      901 ccgggatgac ggcctgctgg tacatgcccc acagcacgcc gcccaggccg gccagcatgg
      961 cgccggcgat gaacacgccg atgaagagat ggcggatgcg atagcccagg ctttcgacca
     1021 tctcgcggtt ttccacgccg gcgcgcacca ggaggccgag cttggtgcgg ttgagcagcc
     1081 acagcatgag caccagcacc aatacgccga tgaccagggc cagcaggcgg aacttctcga
     1141 cggcggcatc gcccatcagc acggcgccgc gcaaggccag aggcaagggc agcggcagcg
     1201 tctgcggccc ccagatggcc ttgatgagct cttcgcccac gatcatgccg cccacggtga
     1261 tgaggatctg cttgagatgc tgcccgtaga ccgggcgcac gatgaggcgc tcgaaggccg
     1321 cgcccaccgc gccggccacc agcatcccga tcacggcggc gggcagcacc gcgccgaggt
     1381 tggcgagcag gttgtccgac tgcgtccagc cggccatgcc gcccagcacg gtgcaggcca
     1441 cataggcgcc cagcgtgatg aacaggccgt tgccgaagtt cagcacgtcc atgaggccga
     1501 agaccagcgt catgccggaa gccacgatga agacgatcat gcccatggcc aggccggcga
     1561 tggtgagcgt gagccaggtc gagaaatcgc cgaccagggg cagggcgacg atggccaggc
     1621 cggcgaccag cagcatgggg atggggtcga gccgcgtgcc gggcaggggg gtgtcgtgcg
     1681 tatcgcgagc gggcgcgttc attgatggct gtccagggag aggcccaaga ggcgcgtttg
     1741 cagcgcctgg tcctgggaga gttcggccat gccgccgtag tggacgaccc ggccgttgtc
     1801 catcacggcg gcctggtcgc ccaggcggcg ggcgaaatcg aagttctgtt cgaccagcag
     1861 tatcgtggtg gcgtcgcgct tgagttcggc gaacgcctcg atcatgttct gcacgatggc
     1921 cggggccagg cccttgctgg gttcgtcgac cagcagcagc cggcgcggct cgatgatggc
     1981 gcgcgccacg gccagcatct gcttctgtcc gccggacagc ttgccggcgg gatacagcca
     2041 gaatttgcgc agcgccggga agaagccgaa gatccattcc aggcgcgccg tgtcgagctc
     2101 ggaagccttg cgcgccttgc gcgcggccag caggatgttt tcctgcacgc tgaggtcgga
     2161 gaagatgccc atgctttcgg gcacgtaggc gacgccctgg tccaccatgt cgggcggggc
     2221 gatgtgctgg ccggcgctgc cgatcacgcg gccttgcagg cgcagttcgc cggacgaggc
     2281 gcgccacagg cccatgatgg tgcgcagggt ggtggtcttg ccggcgccgt tgcggcccag
     2341 cagcatggtg acctgccctg cgggcacggc caggtcgacg ccgtgcagga tatggtaggc
     2401 gccgatgtgc gtgtgcacgt cgcgcagttc gagcagcggc gcggcggact ggctcatgcg
     2461 gtctcccgct cgggcgcggt gcccaggtag gcctgctgca cgatgtccga ggcgattacc
     2521 tcggccgggt tgccgtcggc catgagccgg ccgttgtgca gcaccacgat gcggtcggcc
     2581 agttcgcgca ccacat
//
LOCUS       DASWPO010000267        20987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2831359, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000267 DASWPO010000000
VERSION     DASWPO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20987
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2831359"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VGC69_14000"
     CDS             complement(<1..661)
                     /locus_tag="VGC69_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6492 family protein"
                     /protein_id="HEY0296439.1"
                     /translation="MTQISAILPIKTRGRHYADNIGRCDILFSSLRHFAAPGLFSRFV
                     LIVPHDEVEDIEGYAKAWSDFPIEVVDESLHMGVFAEFSQRHQIRNWHRQQIIKLSAP
                     ALIDTEYFLVFDPDCFATHPMSADTLIVDGRALTHYMPRTADNPGYWESSAKLLGVDP
                     HLDRDGIWMTPAILSRTLCLNLHRRLEAVHGQDWRRVLLSHYMIDWTEYTLYWLNAED
                     EG"
     gene            complement(687..2858)
                     /locus_tag="VGC69_14005"
     CDS             complement(687..2858)
                     /locus_tag="VGC69_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296440.1"
                     /translation="MTVRDHLNTFFYYRKIAAMVFLAIAVLGIASALILPMPYQAQAR
                     LLVLFAGYYDQSNATRGVPVVPAIGELVSVEAQILESPELHRAVVLSELGPNPTQQAI
                     DQRLHDFENRLHLEQTDLANTINLSYTDNDPKRAANALSRLLTEYFRQRATIFISGRA
                     SLLAEQQGEVRKKLDQANAALLAFQQAHGIVSINDQISRAVALENLLTQRKMENDAAL
                     VQDRGGLEALQAATRDVQPDITLFTDNSEIAHTLATMQVSLMQLETQRSELASRYMGS
                     SPFVQQLDKQIADMRSNIGKQKQQLLSATRYGHNSYYDTVQDRLAGMLTNISGEVARQ
                     KELQNQVAEAHARSQNLIALADQLRQMQADRDLLADSFRERARQVEQADIQQEQASKV
                     NSTNVRVIQAPLPPTRHSVSRGLLIAASLVAALVVSALVVLVAATLRETFLSPEQVER
                     ALSLPILSAPIIVRRAAARLGSTPATASPATAPEVASPFSGAPPAAAAAAKPVRGGLS
                     ARIAHMAYGRMASAIYGSSNAASKLVMMLAPGKDEGVHKVIQGMAVEQERRSVHPVLI
                     LDMDVAPEASLYGLVNAQGLIDWPDVEPVNPVSGAGTYARIPGMDFHRVAGHNIVVAR
                     PDAGLLSSSRQQAASLFGALRQYYEYILVHAPPANRSFAGIENVTLVDAVVLVIRAEA
                     TRKPVAQSLKEQVLDAGGKIIGVAMTYRRQYIPEPIYRIFF"
     gene            3127..3846
                     /locus_tag="VGC69_14010"
     CDS             3127..3846
                     /locus_tag="VGC69_14010"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0296441.1"
                     /translation="MMLRLVFMLALASLAGCSSTWVDAQHQQPDRFATWDDAPPATYL
                     IQPGDDLSVTLPFNAELNQKASVAPDGTFTMPLVGALPAAGYSTTQFAGRIDKALAAN
                     GVVADARATISVTQYAGRVYVGGQVGQPGEVPLRAGMTVLQAIEAARGTRDTARTDEI
                     VLIRRGSDGRPMLRTVNIDALTHTGDPNQAVVLQASDTIFVPKSSIAEVDQWVDQHIN
                     QALPFSKGLSYTYTNTTATTP"
     gene            3864..5150
                     /locus_tag="VGC69_14015"
     CDS             3864..5150
                     /locus_tag="VGC69_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296442.1"
                     /translation="MEKAAPQDELAPVFSTDPARPRILRWLVVVLPLFGQTFHYINAL
                     LPLWALSKAFPVLSLPLALWLMRHARFPMTRQLLLSFAWLLLLPSFSAVFYFNESFFT
                     SITAQVKLLPLLYFFSFLALLLILQPTLRELERSFILMGIIVVGVLIVLWAVIPNSWY
                     RSSYSLSTTPLFTSDNRGHRIRMSMYFPIVMLFFCYRRAWFERSWRYLLGALIAFAVT
                     LLIVKTRAMVIGISGVVAINAIIWARPRVKVLLLILAPAALAAMFSVGYLATVFDTSA
                     SSGIDVRLTTIQLASHFLGDSPLRWFFGVGTISPTSRESLITYFSHGFFLADITWLGI
                     VFEYGIFGALLFIFIELRGLAFYRRLRAAVEDDFLGALADYLIYVLLISPFYPPTLTP
                     GETTVILAIFVYVWHAGGFEYNPTGARIPHAQAQPQ"
     gene            5128..6789
                     /locus_tag="VGC69_14020"
     CDS             5128..6789
                     /locus_tag="VGC69_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HEY0296443.1"
                     /translation="MPKLSRNSLYTLPAALMLWSGMAICIAPQDVPLPVAIAVDAAAN
                     RHPISPLIYGLNFGTTQTLQDLRIPINRSGGNSASAYNWQLDARNSGKDWFFESIPAD
                     PQAIYDQYNERFVELTRAADAIPMLTIPMMGRVAKLGQDRKPIASFSVDKYGPQQSYD
                     VDGLRDAGNGIALDGTPITNNDPDDASVPHDAKSEEERVRQLVRKYGNADAKGVRYYL
                     MDNEPSLWQLIHRDIRPIGAHASEIADKVITYSRAVKAADPKALVVAPEEWGWFGYRY
                     SGFDQQYMAEHGTMDHAPDKESETRGMDYVPWLLTQWQQAGHPIDVFSLHFYPQSGEY
                     NEAGEPDTPAIALARNRSTRDLWDPNYKDPTWINSVVALIPLMRQWVDTYYHPGTPIA
                     LTEYDWGGETLMNGATAQADLLGIFGREGLDIATRWGTIDSSMPVYKAIKLYRNYDDH
                     DSGFGTTSVAATVPDPDQVSSFAALRDHDRVMTIMVINKQLDRPAETTIAVNHFADHG
                     KAETWQLADNAISRLHDTRYADGQLHATLPPQSVTHFVLYSTAHP"
     gene            6816..8180
                     /locus_tag="VGC69_14025"
     CDS             6816..8180
                     /locus_tag="VGC69_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEY0296444.1"
                     /translation="MHADAAPAQGRSMIRGSLISLGVRLLDLPSRYGFHLLVAAALGV
                     VDTGRFYIVFSIMVALAGFGRLGMDQALTRQLAMDMASDRPAAVRPAIRRALLSVLLA
                     SVAVAALLAAGAAPFAHDVLKKSDLAVPLALGALAIIPQNLGAALAGALAGLQRVGYS
                     QMIYSWLWPAIFCIVAIPLSLAGLLTTTNALVLTAASFALTALAGAVLLKRALAAVPG
                     AGQPAQAAPSLLRPGLSLFTLELTKLLITSAPAIVLGIVATDRETGLFALTWRMALAI
                     NLLISGVTGMAVPKFAALHARGNTQALEKNAAQAIGLVLCLALPAVACMLIFPTHLLA
                     LFGQGYGQGAATLRILAVGQLAAACFTAMPEMLGMTAHMRALYRINACTLAVLLIGLA
                     ALAPLFGSIGAALATSLALAVNGAAAAWLAHQLLGVAPLARLWREVLGRLRRRAARDG
                     GKTS"
     gene            8177..8596
                     /locus_tag="VGC69_14030"
     CDS             8177..8596
                     /locus_tag="VGC69_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296445.1"
                     /translation="MNRGLAAGALLLLCIAQACAIRAAWAASIDPPRYDSNIFCRLRA
                     NTPEGFSDDAQSQCLARQRMAYQAIRQEWNELPEEIREGCDEYTRLKDPLDYEALYSC
                     IQMQQRYLPLAPDVDDDPPTQSASPSEPRLPKDQDMQ"
     gene            8602..9723
                     /locus_tag="VGC69_14035"
     CDS             8602..9723
                     /locus_tag="VGC69_14035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0296446.1"
                     /translation="MWFSIHLDFLRGMAALVVCAGHIRQLVLPPLTGGTGAGGLTIGD
                     EAVMVFFVLSGYLVGLPILRTAFARHVVDDKKYIVDRLARLWTVLLPALAIGCAIDIY
                     GSTIFPRFYGPDILNPTSWTAFLGNLLFVQQLQGPIQPLGTNVVLWSLAYEFWFYVLA
                     YLIAKAALRDKGWWLYAAATIAMYAAFPVHFDFTFLLWLSPILLPGLVTAFITWAGWV
                     VKPRSKWLLSVGAALAFVVLFGLTKKLTVSRYWGELFVVCITNLLLLCLIVCWSSVAV
                     QEKLNWVAKSYKAVAHSLSSVSYTLYLTHLPLVTLGTAYMKDAHVPAWAMFFILFIAS
                     IVWAGFLYFLFERHTYKLRNFFYAKAQKPDLKRWADRGM"
     gene            9752..10810
                     /locus_tag="VGC69_14040"
     CDS             9752..10810
                     /locus_tag="VGC69_14040"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEY0296447.1"
                     /translation="MFERLIHRNVRARAPLRLGLGGGGTDLKPYCDDHGGVVLNATID
                     RYAYAHLSCREDDRIVFRADDLGRSDELACDLDFDIHQGLTLHRATYALMMKRYNNGQ
                     AMPLDISTTIDVPVGSGLGASSALTVALIEAFALAMRLPLSPYEIAGMAYDLERVHLG
                     MLGGKQDQYAAAFGGLNFIEFLKGDDGRVIVNPLRIHREYLNEFESSLVICFSGQSRE
                     SAQIIEDQVAGLRQMDDKTIESMHEIKAHAISMKTMLLGGHIKQTASILQQSWLSKKN
                     TASAVTNSLVEKLIDVAMDNGAWGGKVSGAGGGGFIMLLTDPSLRHRLIQALNKAGGS
                     AGAVRLTFEGAEAWEVPE"
     gene            10841..12061
                     /locus_tag="VGC69_14045"
     CDS             10841..12061
                     /locus_tag="VGC69_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY0296448.1"
                     /translation="MVEQCAILLGGLGTRLGSLTRETPKPLLPVGGVPFIELLIREAW
                     RMGFRHVVLLAGYKSERVHEFLATMRADLPQGASIEVAIEPEPLGTGGAVAHALPMLD
                     ERFLLLNGDTWFDCNWNELCIDDGAGRATIAVREVPLADRYETIDFAPNGLVRAVVPR
                     GEAPAPPYYVNGGAYCLAREHVAGFGERFSIEQDVLPALTRKGQLRAHRFEGYFLDIG
                     VPESYARSQTEVPAQRRKPALFLDRDGVLNHDDGYVGTIDRFRWIAGAKQAIRLANDL
                     GYYVFVVTNQAGVARGFYPVANVPKLFDWMNAELRAQGAHIDDWRFCPHHPDGEVEAY
                     TGAHPWRKPEPGMLLDLMAHWDIDPAASLMIGDQESDLIAASRAGIAAHQFAGGNLRD
                     FVAPLLTHPARREP"
     gene            12061..13206
                     /locus_tag="VGC69_14050"
     CDS             12061..13206
                     /locus_tag="VGC69_14050"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY0296449.1"
                     /translation="MMKNRVAELEREARQWLIGIAAPLWSSRGRTRAGLFAERMALDG
                     TPNDEYFRTFVQARHVYSMVAAGQAGWTGPWQSLIAETMHAIVTRAKRGDGFFVHRLD
                     RDGAVLDGRADLYDQAFVLFALGTAGGALKDQALFDEAEALLDTIEANWSHPRGGFRE
                     GEIVDASIRRQNPHMHLLEAFSALHAASGRQRFGAAATAIAQLCQTRFIDPASGALLE
                     YFDEDWTPVAGGQGRIAEPGHCFEWAWLFERLAAAGRQEAVALSDALTGFGRRHGIDP
                     DRGIAINEVSTDGGVVNGKARLWPQTERLKVAVARYRRTRSDEDLREVAGAWLGLRAY
                     FLPDVPGLWRDKMNEDGSFIEEMAPGSSLYHISCAINEMGSLRDALA"
     gene            complement(13296..13739)
                     /locus_tag="VGC69_14055"
     CDS             complement(13296..13739)
                     /locus_tag="VGC69_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEY0296450.1"
                     /translation="MGLLDTLTSITSSLSSNGSAAPQQQAGLGGLLPVVLEQLSKYPG
                     GVSGLVAAFEQGGMGQVVGSWVGTGQNAPVSADQLDSVLHPGTVDAIAQQSGQSRGDV
                     LAQLSQILPHIVDHATPGGTVPQGQQQGFDPSALIGMLSKLTGPA"
     gene            13893..14441
                     /locus_tag="VGC69_14060"
     CDS             13893..14441
                     /locus_tag="VGC69_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEY0296451.1"
                     /translation="MTSPFSSPAADSDFSGAALYQACVPVFIRGLRALAAELRRGTAS
                     AAARQFDPSILVHARLAPDMLNLAGQVQRASDTSKLSAERLVGVPSPKFPDTETTMEE
                     LHDRITATIAYLQSLKPEHFQGSEAKTISIQLGETRGDLYLLEFGLPNFYFHLAMAHA
                     ILRHNGVPVGKMDYLGAAAEPK"
     gene            14438..15644
                     /locus_tag="VGC69_14065"
                     /pseudo
     CDS             14438..15644
                     /locus_tag="VGC69_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937755.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15860..17584)
                     /gene="acpA"
                     /locus_tag="VGC69_14070"
     CDS             complement(15860..17584)
                     /gene="acpA"
                     /locus_tag="VGC69_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEY0296452.1"
                     /translation="MSDKPQDTDNKPADESDQAAPRATRRGFLTGMAALGASAAAGDA
                     LAAQEGKAHGGKAALHPADPAFTRALRKHVKNVVVIYAENRSFNNLFGNFPGVEKPLS
                     SLHADDYRQLDRDGTPLATLPPVWGGLVPNKQTVNHVHYEIKQDAPHMTGMYNGPFAL
                     RGPQGEYLPQGVVTRDLWHVFYQNQMQINGGRNDKFAAWADSGGLVMGYYADSAYNLR
                     LWKLAEEYVLCDNFFQGAFGGSFLNHLYLVAARPPLYPDVKNSPVRTQVATLESDDPA
                     DPRLKPDPVKSPASALKGPPVFGPSSLTPDGYGVNTMMPPYWPTRAPRDKRNPALADL
                     SSPAVMVPQDSPHIGDMLDAKGVDWAWYAGGWQAAIDAQPDTPDFPAAPNFQYHHQPF
                     NYYRNLGPDHPEARARRLRDGGLGDMPDTNKFLADVEAGKLPAVTFYKPQGNLNMHAG
                     YADVESGDRHIAHIVDSLRRGSQWQDMVVIITVDENGGWWDHVAPPKGDRWGPGSRVP
                     ALVVSPFAKKGLVDHTVYDTGSILRLITRVFDLPMLEGLKLRDDAMRERGQQPMGDLT
                     NALAFVAA"
     gene            complement(17891..18250)
                     /gene="cyoD"
                     /locus_tag="VGC69_14075"
     CDS             complement(17891..18250)
                     /gene="cyoD"
                     /locus_tag="VGC69_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEY0296453.1"
                     /translation="MSHSHAVDHDHAADSAGHGSLKSYIVGFALSIILTLISFWLVMS
                     GSLTHDTAMTWVVVFAIAQLLVQLIFFLHLGTAKSQQQNTAIFLFTVLILAIAVGGSL
                     WVMHNANVNMMPAMGKM"
     gene            complement(18250..18861)
                     /gene="cyoC"
                     /locus_tag="VGC69_14080"
     CDS             complement(18250..18861)
                     /gene="cyoC"
                     /locus_tag="VGC69_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEY0296454.1"
                     /translation="MTQAVSSSLHDVAHHEHHDDGSKTTLGFWIYLMSDCLIFSVLFA
                     TFAVLGKNTAGGPDGKELFDLSYVLVETMLLLFSSFTFGVAMLNLHAGNKNKVIGWLF
                     ITLLFGAGFVGMEIYEFHHLITEGNGPGRSAFLSAFFTLIGTHGLHVSFGLIWILIMM
                     HQVSRFGLDGMTRRRLACLSLFWHFLDIVWICVFTFIYLFGAL"
     gene            complement(18865..20841)
                     /gene="cyoB"
                     /locus_tag="VGC69_14085"
     CDS             complement(18865..20841)
                     /gene="cyoB"
                     /locus_tag="VGC69_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEY0296455.1"
                     /translation="MFGKLSLESVPYHEPIVMVTLAGVMFGAIVVLAAMTYFRLWKPL
                     WTDWLTSVDHKKIGVMYIFVALIMLLRGFADAIMMRTQLAVAGGDSPGFLPPHHYDQV
                     FTAHGVIMIFFMAMPFIVGLMNVVVPLQIGARDVAYPFLNSLSFWLFVAGVILVMLSL
                     FVGEFAATGWLAYPPLSGLDYSPSVGVDYYIWALQISGLGTTLSGINFIVTILRMRTP
                     GMKLMQMPVFTWTALVTNVLIVAAFPVLTATLALLTADRYLGTHFFTNDGGGNAMMYI
                     NLIWIWGHPEVYILVLPVFGAFSEIIATYSGKPLFGYKAMVYATAAIGVMSFVVWLHH
                     FFTMGSGADVNSFFGIATMIISIPTGVKIFNWLFTMYRGRVQMTAPVYWTLGFIITFV
                     IGGMTGVLLAIPGADFLLHNSLFLVAHFHNVIIGGVVFGCIAALTYWFPKAFGFKLNE
                     TLGKCSFVCWFVGFYLAFVPLYILGLKGMTRRLNHYNNPEWQPYLIVALIGAFVIALG
                     IGFLILQILVSIKDRKKNMDVTGDPWGGRTLEWSTSSPAPFYNFAHLPDVDHLDQFWE
                     DKELGRAYKQPTHYEDIHMPRNTGVGVYMGIFATAMGFGLIWHIWWLAIVGFLGMIVS
                     FIARTYDRDTDYYVPAAEVEAIETARYRKLQQAA"
BASE COUNT         3623 a         6930 c         6762 g         3672 t
ORIGIN      
        1 gcccctcgtc ctcggcgtta agccaataga gcgtgtattc ggtccagtcg atcatatagt
       61 ggctgagcag cacacgccgc cagtcctggc catgcacggc ttccaggcgg cgatgcaggt
      121 tcaggcacag ggtgcgcgac agaatggccg gcgtcatcca gatgccatcg cggtccagat
      181 gcggatcgac gccgagcagc ttggccgagg actcccagta gcccggattg tcggccgtgc
      241 gcggcatgta atgcgtcagc gcacggccgt cgacgatcag cgtgtccgcc gacatcggat
      301 gcgtggcgaa gcagtcggga tcgaacacca ggaaatactc ggtgtcgatc agggccggcg
      361 cggaaagctt gatgatctgc tggcgatgcc agttgcggat ctggtggcgc tgcgagaact
      421 cggcaaacac gcccatgtgc agggactcgt cgaccacttc gatcggaaag tcggaccagg
      481 ccttggcgta gccctcgatg tcctcgactt cgtcgtgagg cacgatgagg acgaaacggg
      541 agaaaagacc tggcgccgcg aaatggcgca gagaagagaa caggatatcg cagcggccga
      601 tattatcggc gtaatgccga ccccgggtct tgataggcaa gattgcactg atctgggtca
      661 tggcatgtcg atcctaggct ggcgaatcag aagaaaattc ggtaaatcgg ctcggggatg
      721 tactgccggc gataggtcat ggcgacgccg atgatcttgc ctcccgcgtc cagcacctgt
      781 tccttcaggc tctgggccac cggcttgcgc gtggcctcgg cccggatcac cagcaccacc
      841 gcgtcgacca gcgtaacgtt ttcgatgccg gcgaaagagc ggttcgccgg cggcgcgtgg
      901 accaggatgt attcataata ctggcgcagc gcgccgaaca ggctggccgc ctgctgccgc
      961 gaggatgaca gcagccccgc atcgggccgc gcgaccacga tgttgtgccc cgcgacccga
     1021 tggaaatcca tgcccggtat ccgcgcgtac gtgcccgcgc ccgatacggg attgaccggc
     1081 tcgacatccg gccagtcgat caagccttgc gcattgacca ggccgtaaag cgaggcctcc
     1141 ggcgccacgt ccatgtcgag gatcaggacg ggatggaccg agcggcgctc ctgttcgacg
     1201 gccatcccct ggatgacctt gtgcacgcct tcgtccttgc cgggcgcaag catcatgacc
     1261 aatttggaag ccgcgttcga actgccatag atggcggacg ccatccggcc atacgccatg
     1321 tgcgcgatgc gggcgctcag cccgccccgg accggcttgg cggccgccgc cgccgcgggc
     1381 ggcgcgcccg agaatgggga ggcgacctcc ggcgcggtgg ctggcgacgc ggtagctggc
     1441 gtcgagccga gccgcgccgc ggctcgacgc acgatgatgg gcgcgctcag tatgggtagc
     1501 gacagcgcgc gctcgacctg ttcggggctg aggaaggtct cgcgcaaagt ggccgccacc
     1561 agcacgacca gggcggagac caccagcgcg gcgacgaggc tcgccgcgat cagcaacccg
     1621 cgcgaaacgc tatggcgcgt cggcggaagc ggcgcctgga tgacgcggac gttggtgctg
     1681 ttgaccttgc tggcctgttc ctgctggatg tccgcctgct cgacctggcg ggctctttcg
     1741 cggaagctgt cggccagcag atcgcggtcg gcctgcatct ggcgcagctg gtcggccagc
     1801 gcgatcaggt tttgcgaccg ggcatgcgcc tcggcgacct ggttctgcag ttctttctgg
     1861 cgggcgacct cgccggaaat attggtcagc atgcccgcca gcctgtcctg caccgtgtcg
     1921 tagtagctgt tgtggccgta gcgcgtggcg ctgagcagct gctgcttctg cttgccgatg
     1981 ttcgaacgca tgtccgctat ctgcttgtcg agctgctgca cgaaaggcga actgcccatg
     2041 taacgggacg ccaactccga gcgctgcgtt tcgagctgca tcagcgaaac ctgcatggtg
     2101 gccagcgtat gcgcgatctc ggaattgtcg gtaaagagcg tgatgtccgg ctgcacgtcc
     2161 cgggtggcgg cctgcagcgc ttcaagcccg ccgcggtcct ggaccagcgc ggcgtcgttt
     2221 tccatcttgc gctgggtcag cagattctcg agcgccacgg cgcgcgagat ctggtcattg
     2281 atgctgacga tgccatgcgc ctgctggaaa gccagcaggg cggcgttggc ctggtccagc
     2341 ttcttgcgga cctcgccctg ctgttcggcc agcagggaag cccggccgga gatgaaaatg
     2401 gtcgcgcgct ggcggaaata ttcggtcagc agcctggaca aggcattggc cgcccgcttg
     2461 gggtcgttgt cggtatagga caggttgatg gtgttggcga ggtcggtctg ctcgagatgc
     2521 aaacggtttt cgaagtcgtg cagcctctgg tcgatcgcct gctgggtcgg gttgggcccg
     2581 agctccgaca ggacgactgc gcggtgcaac tccgggcttt ccaggatctg ggcctcgacg
     2641 ctgaccagtt caccgatggc gggcaccacg ggaacgcccc gggttgcgtt ggactggtcg
     2701 taatagccgg cgaacagcac gagcagcctc gcctgggcct ggtagggcat gggcaggatc
     2761 agcgcgctgg cgatgcccag taccgcaatg gccaggaaca ccatggcggc gatcttgcgg
     2821 taatagaaaa aggtgttgag atggtctcgg accgtcaagc tggcggcgac cctgtggtct
     2881 ggtctcgttc gagtggatat aaccatatag ggggggattc gatgccagac ctctgtttca
     2941 gcccgaactc tacccacatt ccggccagtt gtggaaaaaa tgcgcaaaat gacaagcgct
     3001 gtgacctata ctgcagcaac attttataat atttcattct atccacacgc cgcttaagcg
     3061 ccacgccacg gccttccagg cggcactccg agaggttggc cgcgcatgcc ttcgccaagc
     3121 gccccgatga tgcttcgcct ggtcttcatg ctggcgctgg ccagcctggc cggctgcagt
     3181 tcgacctggg tagacgcgca acaccaacag cccgaccgct tcgcaacctg ggacgacgct
     3241 ccccccgcca cctacctcat ccagccgggc gacgaccttt cggtcaccct gcccttcaat
     3301 gccgaactga accagaaggc gtcggtggcg cccgacggaa ccttcaccat gccgctggtc
     3361 ggcgccctcc cggccgccgg ctactcgacc acgcagttcg ccggccgcat cgacaaggcg
     3421 ctggcggcca atggcgtggt cgccgacgcg cgcgccacga tcagcgtcac ccagtacgcc
     3481 ggccgcgtgt atgtgggcgg ccaggtcggg cagccgggcg aggtgccgct gcgcgcgggc
     3541 atgaccgtcc tgcaggccat cgaggcggcc cgcgggacgc gcgacacggc gcgcaccgat
     3601 gaaatcgtgc tgatccggcg cggctcggac ggacggccca tgctgcgcac ggtcaacatc
     3661 gacgcgctga cgcacacggg cgatccgaac caggcggtgg tcctgcaggc ctccgacacc
     3721 atcttcgtgc cgaaatcctc gatcgccgag gtcgaccaat gggtcgacca gcacatcaac
     3781 caggctttgc cgttcagcaa gggcctgagc tacacctaca cgaacacgac cgcgaccacc
     3841 ccctgagccc cctggaccac ccgatggaaa aggccgcacc ccaggacgag ctcgcccccg
     3901 tcttctcgac cgatcccgcc aggccgcgca tcctgcggtg gctggtcgtc gtcctgcccc
     3961 tgttcggcca gaccttccac tacatcaacg ccctgctgcc gctctgggcc ctgtccaagg
     4021 ccttccccgt gctgtcgctg ccgctggccc tgtggctgat gcgccacgcg cgctttccca
     4081 tgacccggca attgcttctc agcttcgcct ggctgctgct gctgccgagc ttttccgcgg
     4141 tgttctattt caacgaaagc ttcttcacca gcatcacggc gcaggtcaag ctgctgccgc
     4201 ttttgtattt cttctctttc ctggccttgc tgctcatcct gcagccgacc ctgcgcgagc
     4261 tggaacgcag cttcatcctc atgggcatca tcgtggtcgg cgtgctgatc gtgctctggg
     4321 ccgtgatccc gaacagctgg tatcgaagct cctactcgct cagcaccacg ccgctcttca
     4381 ccagcgacaa ccgcggccac cgcatccgca tgtccatgta cttccccatc gtcatgctgt
     4441 tcttctgcta ccgcagggcc tggttcgagc gcagctggcg ctatctgctg ggcgcgctca
     4501 tcgccttcgc cgtcacgctc ctgatcgtca agacgcgcgc catggtcatc ggcatctccg
     4561 gcgtcgtggc catcaacgcc atcatctggg cgaggccgcg ggtcaaagtg ctgctgctga
     4621 tcctcgcccc ggccgcgctg gcggccatgt tctcggtcgg ctatctggcc actgtcttcg
     4681 acaccagcgc cagcagcggc atcgacgtgc ggctgaccac catacagctg gcgtcccact
     4741 tcctgggcga cagtccgctg cgctggttct tcggcgtggg cacgatcagc ccgaccagcc
     4801 gcgagagcct gatcacctat ttcagccacg gattcttcct ggccgacatt acctggctgg
     4861 gcatcgtctt cgagtacggc atcttcggcg cgctgctgtt catcttcatc gaactgcgcg
     4921 gcctggcctt ctaccggcgc ttgcgggccg cggtcgagga cgacttcctc ggcgccctgg
     4981 ccgactatct catctacgtc ctgctgatct cgcccttcta tccgccgacg ctcacgcccg
     5041 gcgagaccac ggtcatcctc gcgatcttcg tctatgtgtg gcacgcgggc ggattcgaat
     5101 acaaccccac cggcgcccga atcccgcatg cccaagctca gccgcaatag cctctatacc
     5161 ctgccggcgg cgctgatgct ctggtcgggc atggccatct gcatcgcccc gcaggacgtg
     5221 ccgctgccgg tggccatcgc ggtcgacgcc gccgccaacc gccatccgat cagcccgctg
     5281 atctacggcc tgaacttcgg cacgacgcaa accctgcagg acttgcgcat accgatcaac
     5341 cgttcgggcg gcaacagcgc ctcggcctac aactggcaac tcgatgcccg caactcgggc
     5401 aaggactggt ttttcgaaag cataccggcc gacccgcaag ccatctacga ccagtacaac
     5461 gagcgcttcg tggagcttac ccgggcggcg gatgccatcc ccatgctgac catcccgatg
     5521 atgggccggg tggccaagct cggccaggac cgcaaaccga tcgccagttt ctcggtcgac
     5581 aagtacgggc cgcagcaaag ctacgatgtc gatggcctgc gggatgccgg caacggcatt
     5641 gcgctcgacg gcacgcccat caccaacaac gacccggacg acgcctcggt gccgcacgac
     5701 gcgaagagcg aagaagaacg ggtcaggcaa ctggtccgga agtacggcaa tgccgacgcg
     5761 aaaggcgtgc gctattacct gatggataac gagcccagcc tctggcagct catccaccgc
     5821 gacatccgcc ccatcggcgc ccacgccagc gagatcgcgg acaaggtgat cacctattcc
     5881 cgcgccgtga aggcagcgga tccgaaggcc ttggtcgtgg cgccggaaga atggggctgg
     5941 ttcggctatc ggtacagcgg cttcgatcag caatacatgg ccgagcacgg gaccatggac
     6001 cacgcccccg acaaggaaag cgaaacccgg ggcatggatt acgtaccctg gctgctcacg
     6061 caatggcaac aggccggcca tcccatcgac gtcttcagcc tgcacttcta cccccagagc
     6121 ggcgaataca acgaagcggg cgaacccgac acaccggcca tcgccctcgc ccgcaaccgc
     6181 tccacgcgcg acctgtggga cccgaactac aaggatccga cctggataaa cagcgtggtg
     6241 gccctgatcc cgttgatgcg gcagtgggtg gacacctatt accaccccgg cacgccgatc
     6301 gccctcaccg aatacgactg gggcggcgag accttgatga acggcgccac ggcccaggcc
     6361 gacctgctcg gcattttcgg ccgcgaaggg ctggacatcg ccacccgctg gggcactatc
     6421 gattcaagca tgccggtcta caaggccatc aagctctacc gcaactacga cgaccatgac
     6481 tcgggcttcg gcaccaccag cgtagccgcc accgtgcccg acccggatca ggtctcgtcc
     6541 ttcgcggcgc tgcgcgacca cgatcgcgtc atgaccatca tggtcatcaa caaacagctc
     6601 gaccggccgg ccgagaccac catcgcggtc aatcatttcg cggaccacgg caaggccgag
     6661 acctggcagc tcgcggacaa tgccatatcc cgcctgcacg acacccgcta tgccgacggc
     6721 cagttgcacg ccaccctgcc gccgcaaagc gtgacccact tcgtcctgta cagcacggcg
     6781 cacccatgac cagacggcaa gacggaccaa ccggcatgca cgcagacgcc gcccccgcgc
     6841 aaggccgctc catgatccgg ggctcgctca tcagcctggg cgtgcggctg ctcgacctgc
     6901 cgagccgcta cggcttccat ctgctggtcg cggccgcgct gggcgtggtc gacaccgggc
     6961 gcttctacat cgtattcagc atcatggtgg cgctggccgg tttcggccgg ctgggcatgg
     7021 accaggcgct gacccggcag ctcgccatgg acatggcatc ggatcgcccc gccgccgtgc
     7081 gccccgcgat ccggcgcgcg ctgctctcgg tcctgctggc ctccgtggcc gtcgcggcgc
     7141 tgctcgcggc gggcgccgcg cccttcgccc atgatgtgct gaaaaaaagc gacctggccg
     7201 tgccgctggc cctgggcgcg cttgccatca ttccgcagaa cctgggcgcg gccctggccg
     7261 gcgcgctggc cgggctgcag cgcgtgggct acagccagat gatctattcc tggctctggc
     7321 cggccatctt ctgcatcgtc gccattccgc tgagcctggc aggcctgctc accacgacca
     7381 acgccctggt cttgaccgcc gcgagcttcg ccctgaccgc gctggccggg gccgtcctgc
     7441 tcaagcgcgc cctcgccgcc gtgcccggtg ccgggcagcc tgcgcaagcg gcgccctcgc
     7501 tgctgcggcc gggcctgtcg ctctttaccc tggaactgac caagctgctc atcacctcgg
     7561 cgcccgccat cgtgctcggc atcgtggcca cggaccgcga aaccggcctg ttcgccctga
     7621 cctggcgcat ggccctggcc atcaatctgc tgatcagcgg cgtgaccggc atggccgtgc
     7681 ccaaattcgc cgcgctgcac gcgcgcggca acacgcaagc gctggaaaag aacgcggccc
     7741 aggccatcgg cctggtgcta tgcctggcgc tgccggcggt ggcctgcatg ctgatcttcc
     7801 ccacgcacct gctggccctg ttcggccagg gctacggcca gggcgcggcc acgctgcgca
     7861 tcctcgcggt cggccaattg gcggccgcct gctttaccgc catgcccgag atgctgggca
     7921 tgacggcgca catgagggcg ctctaccgga tcaatgcctg caccctggcc gtgctgctca
     7981 tcggcctggc ggcgctggcg ccgctattcg gcagcatagg ggcggcgctg gcgacttcgc
     8041 tggccctggc cgtgaacggc gccgccgcgg cctggctggc gcaccagctg ctgggcgtcg
     8101 cgccgctcgc ccggctatgg cgcgaggtgc tcggccgcct gcgccgccgc gccgcgcgcg
     8161 atgggggaaa aacatcatga accgaggcct ggccgcgggc gccctcctgc tcctgtgcat
     8221 cgcgcaggcc tgcgcgatcc gcgcggcctg ggcggccagc atcgatccgc cgcgctacga
     8281 ctcgaacatc ttctgccgtc tgcgggccaa tacgccggaa ggcttttcgg atgacgccca
     8341 atcgcaatgc ctggcccgtc aacgcatggc ctaccaagcc atccggcaag aatggaacga
     8401 attgcccgaa gaaatccggg aaggctgcga cgagtacacc cggctcaagg atccgctgga
     8461 ctacgaggcg ctatacagct gcatccagat gcagcagcgc tacctccctc ttgcgccgga
     8521 cgtggacgac gatccgccaa cacagtccgc cagtccatca gaaccacgtc ttcctaaaga
     8581 tcaggatatg cagtgaagaa catgtggttt tccatacacc tggacttcct tcgaggaatg
     8641 gcggcgctcg tcgtctgtgc cggtcacatc aggcagctgg tgctgccgcc gctcacaggc
     8701 ggcacaggcg ccggcggcct cacaataggc gacgaagcgg tgatggtgtt cttcgtgctt
     8761 tccggctacc tggtcggcct gccgatcttg cggaccgctt tcgcccggca cgtggtagac
     8821 gacaagaaat acattgtgga caggctggcc aggctctgga ccgtcctcct gcccgcgctg
     8881 gcaatcggtt gtgcgatcga tatatatggc agcacgatct ttccacgctt ctacgggccg
     8941 gacatcctga accccacgag ctggacggct tttctgggta acctgctgtt cgtgcagcaa
     9001 ctccaagggc cgatccagcc actgggaacg aacgtcgtgc tgtggagcct ggcgtacgaa
     9061 ttttggttct acgtcctggc ttacctgatc gcaaaggcag ccctgcgcga caagggttgg
     9121 tggctctacg cggcggccac catcgcgatg tacgcggcat ttcccgttca cttcgatttc
     9181 acgtttctgc tttggctcag cccgatcttg ctaccgggcc tggtaaccgc atttatcaca
     9241 tgggcgggat gggtggtcaa gccccgtagc aagtggctcc tgtccgtggg cgccgcactg
     9301 gcttttgtcg ttcttttcgg tttaaccaag aagctgaccg tttcgaggta ctggggagaa
     9361 ttgtttgtcg tctgcataac aaacttactt ctgctctgct tgatcgtctg ttggtcgtcg
     9421 gtggcggtgc aagaaaagct gaactgggtt gccaagtcgt acaaggccgt ggcgcacagc
     9481 ttgtcgtctg tttcatacac tctgtacctg acacacttgc cgctggttac gctaggcact
     9541 gcctacatga aggatgcgca cgtcccggct tgggccatgt tcttcattct attcatcgca
     9601 tccatcgtat gggccggttt cttgtacttc ctgttcgagc ggcacactta caagctcagg
     9661 aattttttct atgccaaagc gcaaaagccc gatctgaaaa gatgggcgga tagaggcatg
     9721 taaagcctgg ccaaccaaac gaggtcatcg aatgtttgaa cgactgatac accgaaacgt
     9781 acgcgcccgg gcgccgttgc gactggggct gggcggcggc ggcaccgacc tcaagcccta
     9841 ctgcgacgat catggcgggg tggtgctgaa cgcgacgatc gaccgttatg cctacgctca
     9901 cctttcctgc cgcgaggatg accggatcgt gttccgcgcc gatgatctcg gacgctcgga
     9961 cgaactggcc tgcgatctcg atttcgatat tcaccagggc ctgacgctgc accgcgccac
    10021 ctacgccctg atgatgaagc gctacaacaa tggccaggcc atgccattgg acatctcgac
    10081 cacgatcgac gttccggtcg ggtccggact gggcgcctcc tcggccttga cggtggcgct
    10141 gatcgaggcg ttcgccctgg ccatgcgcct gccgctcagc ccttacgaaa tcgccggcat
    10201 ggcctatgac ctcgaacgcg tgcacctggg gatgctcggc ggcaagcagg accaatacgc
    10261 cgcggctttc ggcggcctca acttcatcga gttcctcaag ggcgacgacg gcagagtgat
    10321 cgtcaatccg ctgcgcatcc accgcgaata cctcaacgag ttcgaatcct cgctggtgat
    10381 ctgcttcagc ggccagtcgc gggagtccgc gcagatcatc gaggaccagg tcgccggcct
    10441 caggcagatg gacgacaaga ccatcgaatc gatgcacgag atcaaggctc acgccatcag
    10501 catgaaaacg atgctgctcg gcggccatat aaagcagacg gccagcatcc tgcaacagtc
    10561 ctggctgtcg aagaagaaca cggcctcggc cgtcaccaac agcctcgtcg aaaaactgat
    10621 cgacgtggcg atggacaacg gcgcatgggg cggcaaggtt tccggcgcgg gcggcggcgg
    10681 cttcatcatg ctgctcaccg acccgtcgct gcgccatcgc ctgattcagg cgctcaacaa
    10741 ggccggcggc agcgcgggcg cggtgcgcct gacgttcgaa ggcgccgagg cctgggaggt
    10801 tccggaatga agcgcgcgca aaaaaaacgg aaccacggcc atggttgagc aatgcgccat
    10861 tctgctgggc ggcctgggaa cccgcctcgg gagcctgacg cgcgagacgc ccaagccctt
    10921 gctgcccgtt ggcggtgtgc cgttcatcga gttgctgatc cgcgaggcat ggcgcatggg
    10981 ttttcgccat gttgttctgc ttgcaggcta caagtccgag cgggtgcacg aattcctggc
    11041 gacgatgcgc gcggacctgc cgcaaggggc ctcgatcgag gtcgccatcg agcccgaacc
    11101 gctcggcacc ggcggcgcgg tcgcccatgc gctgcccatg ctggatgagc ggtttctgct
    11161 gctcaacggc gatacctggt tcgactgcaa ctggaatgaa ctgtgcatcg acgacggcgc
    11221 cggccgcgcc acgatagcgg taagagaggt gcccctggcc gaccgctacg agacgataga
    11281 tttcgcgcca aatggcctgg tccgcgccgt ggtcccccgc ggagaggcgc cagcgccgcc
    11341 ctactatgtc aacggcggcg cttattgcct agcccgggag catgtggcgg gtttcggcga
    11401 acggttttcg atcgagcagg acgtcctgcc cgcgctcact cgcaaggggc aattgcgcgc
    11461 gcaccgcttc gaaggctatt tcctcgatat cggcgtgccc gaatcctatg cgcgcagcca
    11521 gaccgaggtg cccgcgcagc gccgcaagcc cgccctgttc ctcgaccgcg acggcgtact
    11581 caaccatgac gacggctatg tcggcacgat agaccggttt cgctggatcg ccggcgcgaa
    11641 gcaggccatc cggctcgcca acgatctggg ctattacgtt ttcgtcgtga ccaaccaggc
    11701 gggcgttgcg cgcggcttct atccggtggc gaacgtaccg aaactgttcg actggatgaa
    11761 cgccgaactg cgggcgcaag gcgcccatat cgacgattgg cgcttctgtc cccaccatcc
    11821 cgacggcgag gtcgaagcct acacaggcgc ccatccttgg cgcaaacccg agccgggcat
    11881 gctgctcgac ctgatggcgc attgggacat cgacccggcg gcaagcctga tgatcgggga
    11941 tcaggaatcc gacttgatcg ccgcttcgcg cgcgggaatc gccgcgcacc agtttgccgg
    12001 cggaaacctg cgcgatttcg tcgcgcccct gctcacccac ccagccagac gggaaccctg
    12061 atgatgaaaa accgcgttgc cgaactcgaa cgggaagcac ggcagtggct gatcgggatc
    12121 gccgcgccgc tctggagcag ccggggccgc acgcgcgcgg gcctgttcgc cgaacgcatg
    12181 gcgctcgacg gcacgcctaa cgacgaatac ttccgcacat tcgtccaggc gcgccacgtc
    12241 tattcgatgg tcgccgccgg ccaagccggc tggacgggtc cgtggcaatc gctgatcgcg
    12301 gaaacgatgc acgcgatcgt cacccgcgca aagcgcggcg acggcttctt cgtccaccgg
    12361 ctcgaccgcg acggcgccgt gctcgacggg cgggccgatc tttacgatca ggctttcgtg
    12421 ctgttcgcgc tcggtaccgc gggcggcgcg ctgaaggacc aagcgctgtt cgacgaggcc
    12481 gaagccttgc tcgacacgat cgaggccaat tggtcgcacc cgcgcggcgg cttccgtgaa
    12541 ggcgagatcg tcgatgcctc gatccgccgg cagaacccgc acatgcatct gctggaagca
    12601 ttcagcgcgc tacacgccgc cagcggacgg cagcgcttcg gcgctgccgc gaccgctatt
    12661 gcgcaactgt gccaaacccg cttcatcgat cccgcctccg gcgcgctgct cgagtatttc
    12721 gacgaagact ggacaccggt ggcgggcggc cagggccgca tcgccgaacc cgggcactgc
    12781 ttcgaatggg catggctgtt cgagcggctg gcggccgcgg gccggcaaga ggccgtggca
    12841 ttgtccgacg cgttgaccgg cttcggccgc cgccatggca tcgatccgga ccggggcatc
    12901 gccatcaacg aagtgtcgac cgacggcggg gtggtcaacg gcaaggcgcg cctttggccg
    12961 cagaccgagc ggctcaaggt cgcggtcgcg cgctaccgcc gcacccgcag cgacgaagat
    13021 ctgcgcgagg tggccggcgc atggctgggc ctgcgcgcct acttcctgcc ggacgtcccg
    13081 ggcttgtggc gcgacaagat gaacgaggac ggcagcttca tcgaggagat ggcccccggc
    13141 agttcgctgt accacatttc gtgcgcgatc aatgagatgg gcagcctgcg cgacgccctc
    13201 gcgtgacccc ccgcccgccg gcggccgctt ccggcgacgg ctgcgtccca ccctggaaaa
    13261 cggcccttgc gggccgtttt gcgcaggcga atcggtcagg cgggtccggt cagcttggag
    13321 agcatgccga tcagggcgct ggggtcgaaa ccctgctgct gcccttgcgg cacggtgccg
    13381 ccgggcgtgg catggtcgac gatgtgcggc aggatctggc tcagttgcgc cagcacgtcg
    13441 ccgcgcgact gcccgctctg ctgggcgatc gcatcgaccg tgccgggatg cagcacggag
    13501 tcgagctggt cggcgctgac gggtgcgttc tggcccgtgc cgacccagga accgacgacc
    13561 tggcccatgc cgccctgctc aaacgcggcc accaggccgg acacgccgcc ggggtatttg
    13621 gacaattgct ccagcacaac gggcaggaga ccgcccaggc cggcttgctg ctgaggcgca
    13681 gcgctaccat tggaagacaa agacgaggtg atcgaggtca gagtatcgag cagtcccatg
    13741 acggaaatcc tcatgaagag ttgaggggga agcagccgat cgattctaaa ggcctttttt
    13801 gtccggaagg taacagtctg ttgaccgggg tccactttcc cggcgctgta ccatagcgac
    13861 attcgcgtct ctcccctgct ggaatctctt cgatgacctc ccccttttcc tctcccgccg
    13921 ccgactccga cttctccggg gcagccctgt accaggcctg cgtgccggtc ttcatccgcg
    13981 ggctgcgcgc cctggccgcc gaactgcgcc gcggcacggc cagcgccgcg gcgcgccagt
    14041 tcgacccgtc catcctggtc catgcccgcc tggcgcccga catgctgaac ctggccggcc
    14101 aggtgcaacg cgccagcgat acctccaagc tgtcggccga gcgcctcgtc ggcgtgcctt
    14161 cgcccaagtt tcccgacacc gaaaccacga tggaggaact gcacgaccgc atcaccgcca
    14221 ccatcgccta cctgcaaagc ctgaaacccg agcacttcca gggcagcgaa gccaagacca
    14281 tctcgatcca gctgggcgag acgcgcggcg acctctacct gctggagttc ggcctgccca
    14341 acttctactt ccacctcgcc atggcgcacg ccatcctgcg gcacaacggc gtgccggtcg
    14401 gcaagatgga ctacctgggc gccgcagccg agcctaaatg accctcggcg ccgacatcgc
    14461 gcagcaccgc ctggggctgg tgctgttcgc cctggcgatg ggtggctttg ccatcggcac
    14521 caccgaattc gccaccatga gcctgctgcc ctatttcgcg cacagcctgg gaatcgacgc
    14581 gccgacggcc ggccacgtca tcagcgccta tgcgctgggc gtggtggtgg gcgcgcccat
    14641 cctggccgtg ctgggcgcgc gcgtgccgcg ccgcacgctg gctgctcgtc ctgctgatgg
    14701 tcctgttcac cctcggcaac ggcctgagcg gcctggcgcc cgactaccac tggatgctgc
    14761 tgttccgctt catcgcgggc gtgccccacg gcgcatactt cggcttcgcc gccctgatgg
    14821 ccgcctcgct cgtgcccgag aaccggcgca cctgggccgt gggccgcatg ttcctggggc
    14881 tgacttgcgc caccatcctc ggcgtgccca tggccgactg gctgggacag gccgtgggct
    14941 ggcgctggag tttcggcctg gtggccctgc tgggcctgat caccatgacc tgcgtgcgcc
    15001 tgttcgcgcc ccacgcgccg gccgacccgc aagccagccc gctgcgcgaa ctgggcgccc
    15061 tggcgcgcgg ccaggtgtgg ctgacgctgg gcatcggcgc cgtcggcttc ggcggcctgt
    15121 tcgcggtcta cacctacctg gccgacatcc tccacgccgt cacgcgggtc tcgcccgcca
    15181 ccacgccgct ggtgctgagc gtgctgggca tcggcctgac ggcgggcaat ctgttcatcc
    15241 cgcgcttcgc cgaccgcgcc ctcatgcgca cggcgggcgc cctgctcgtc tggagcgcgg
    15301 tctcgctggc cctgttcccc ttgatggcgg gccggatctc gaccgtcctg ccttcggtcc
    15361 tcatgatcgg catcggcggc gcgctggcca ccgtgctgca gactcggctc atggacgtgg
    15421 ccgaagacgc gcagggcctg gccgccgcgc tgaaccattc ggccttcaac atcgccaatg
    15481 cgctgggccc cttcctgggc ggcctggcga tcgccggcgg ccacggctgg acttcggtcg
    15541 gctgggtggg ctgcgcgctg gcgctgggcg gtcttgtgct gtgggcggtg tctgtggggg
    15601 tggatcgcta ctacagctct cgtctctgcg aggctggggc ctgagtcacg ctgagcctag
    15661 gttctaggaa ccgagcatgc gctccaagac gccggctcac agcctcttca catgacgggt
    15721 ttgggaaccg ccgtattagg ctggcggagc cgaggacccc ggagcaagcc gaagcgcagc
    15781 ccccacgggg ggcgagcatc ggaattgcgc agggcagcct cggcggctca agaactcaat
    15841 aaccacgcaa cgacaaacac tacgcagcaa caaacgccag cgcattcgtc aagtccccca
    15901 tcggctgctg cccccgctcg cgcatcgcat catcgcgcag cttcagcccc tcgagcatgg
    15961 gcagatcgaa cacccgcgta atcagccgca gaatcgaccc cgtgtcatac accgtgtgat
    16021 ccaccagccc cttcttcgcg aagggcgaca ccaccagcgc cggcacgcgc gagccggggc
    16081 cccagcggtc gcccttgggc ggcgccacgt ggtcccacca gccgccgttc tcgtccacgg
    16141 tgatgatcac caccatgtcc tgccattggc tgccgcggcg caggctgtcg acgatgtgcg
    16201 cgatatggcg gtcgcccgac tccacgtcgg catagccggc atgcatgttc aggttgccct
    16261 gcggcttgta gaaggtcacc gccggcagct tgccggcctc gacgtcggcc aggaacttgt
    16321 tggtgtcggg catatcgccc aggccgccgt cgcgcaggcg cctggcgcgc gcctcgggat
    16381 ggtccggacc gagattcctg tagtagttga agggctggtg gtggtactgg aaattcggcg
    16441 cggccgggaa gtccggcgtg tcgggctggg cgtcgatggc ggcctgccag ccgccggcat
    16501 accaggccca gtccaccccc ttggcgtcga gcatgtcgcc gatgtgcggg ctgtcctgcg
    16561 gcaccatcac cgcggggctg ctcaggtcgg ccagtgccgg attgcgcttg tcgcgcggcg
    16621 cgcgggtcgg ccagtagggc ggcatcatgg tgttgacgcc gtagccgtcg ggcgtgagcg
    16681 agctggggcc gaacaccggc gggcccttca aggcgctggc cggcgatttc acggggtcgg
    16741 gcttcaagcg cgggtcggcc gggtcgtcgc tttccagggt ggcgacctgc gtgcggaccg
    16801 gcgagttctt cacgtcggga taaagcggcg ggcgcgcggc caccaggtac aggtggttga
    16861 ggaaggagcc gccgaaggcg ccctggaaga agttgtcgca cagcacgtac tcctcggcca
    16921 gcttccacag gcgcaggttg taggcgctgt cggcgtaata gcccatcacc aggccgcccg
    16981 aatcggccca ggcggcgaac ttgtcgttcc tgccgccgtt gatctgcatc tggttctggt
    17041 agaacacgtg ccacaggtcg cgcgtgacca cgccctgcgg caggtattcg ccctgggggc
    17101 cgcgcagcgc gaaggggccg ttgtacatcc ccgtcatgtg cggcgcgtcc tgcttgatct
    17161 cgtaatggac gtggttgacc gtctgcttgt tgggcaccag gccgccccac accggcggca
    17221 gcgtcgccag cggcgtgccg tcgcggtcga gctgccggta gtcgtcggca tgcagcgagg
    17281 acagcggttt ttcgacgccg gggaagttgc cgaacaggtt gttgaagctg cggttttcgg
    17341 cgtagatgac cacgacgttt ttcacgtgct tgcgcaaggc gcgcgtgaag gccggatcgg
    17401 cgggatgcag cgccgccttg ccgccgtgcg ccttgccctc ctgggcagcc agcgcatcgc
    17461 ccgccgccgc gctggcgccc agcgcggcca tgccggtcag gaatccgcgg cgcgtggcgc
    17521 gcggcgcggc ctggtcggat tcgtcggcgg gcttgttgtc cgtgtcttgc ggcttgtcgc
    17581 tcatgttcca cctcgtatga aaaatggcca tcgattcgtg agcttagagc cgtcatattt
    17641 acgtcatgtg acggatttct tgcagtctgc ctacatcgtg cgggggggtg ggtctgtctg
    17701 cgttcggttg ggttcttgag acgccgaggc tgccctgcgc aattccgatg ctcgccccca
    17761 ctgggggctg tgcttcggat tgctccgggg tcctcggctt gcgttttgcc caagcccgaa
    17821 aagtaaaaag cccacgcttg gcgtgggctt cgtcgcctcg ctgatggagg caaagggggc
    17881 gcgttgcgtc ttacatctta cccatggcgg gcatcatgtt gacgttggcg ttgtgcatca
    17941 cccacagcga gccgccgacg gcgatcgcca ggatcagcac ggtgaacagg aagatcgcgg
    18001 tgttctgctg ctgcgacttg gcggtgccca ggtgcaggaa gaagatcagc tggaccagca
    18061 gctgggcgat ggcgaacacg accacccagg tcatggccgt gtcatgggtc agcgaaccgc
    18121 tcatcaccag ccagaacgag atcagcgtca ggatgatgga gagcgcgaag ccgacgatgt
    18181 aggacttcag gctgccatga ccggcagaat cagcggcgtg gtcgtgatcg accgcatgag
    18241 aatgcgacat cagagggctc cgaacagata gatgaaggtg aacacgcaga tccagacgat
    18301 gtccagaaag tgccagaaca ggctcaggca ggccaggcgg cggcgggtca tgccgtccag
    18361 gccgaagcgg ctcacctggt gcatcatgat caggatccag atcaggccga aggagacgtg
    18421 caggccgtgg gtgccgatca gcgtgaagaa cgccgacagg aaggcgctgc ggccggggcc
    18481 gttgccttcc gtgatcaggt ggtggaattc gtagatttcc atgcccacga agccggcgcc
    18541 gaacagcagg gtgatgaaca gccagccgat caccttgttc ttgttgccgg catgcaggtt
    18601 gagcatggcc acgccgaagg tgaagctgga aaacagcagc agcatggttt cgaccagcac
    18661 atacgacagg tcgaagagtt ccttgccgtc cgggccgccg gcggtgttct tgcccaggac
    18721 ggcgaaggtc gcgaacagca ctgaaaagat caggcagtcg ctcatcaggt agatccagaa
    18781 accgagcgtg gtcttggatc cgtcgtcgtg gtgctcgtga tgcgccacgt cgtgcaggga
    18841 ggaagatacg gcttgggtca tgactcaggc agcctgttgc agtttgcgat agcgggcggt
    18901 ttcgatggct tcgacttccg cggccgggac gtagtaatcg gtgtcgcggt cgtaggtgcg
    18961 cgcgatgaac gagacgatca tgccgaggaa accgacgatg gccagccacc agatgtgcca
    19021 gatcaggcca aagcccatgg ccgtggcgaa gatgcccatg tagacgccga cgcccgtgtt
    19081 gcgcggcatg tggatgtctt catagtgggt cggctgcttg taggcgcggc caagctcctt
    19141 gtcttcccag aactggtcca ggtggtcgac atcgggcaag tgggcgaagt tgtagaacgg
    19201 cgcgggcgag gaggtcgacc attcgagcgt gcggccgccc cacgggtcgc cggtgacgtc
    19261 catgttcttc ttgcggtcct tgatgctgac caggatctgc aggatcagga agccgatgcc
    19321 cagcgcgatg acgaaggcgc cgatcagggc cacgatcagg tagggctgcc actcggggtt
    19381 gttgtagtgg ttcaggcggc gcgtcatgcc cttcaggccc aggatgtaca ggggcacgaa
    19441 ggccaggtag aagccgacga accagcacac gaacgagcac ttgcccaggg tttcgttgag
    19501 cttgaagccg aaggccttgg ggaaccagta ggtcagcgcg gcgatacagc cgaacaccac
    19561 gccgccgatg atcacgttgt ggaagtgcgc gaccaggaac aggctgttgt gcagcaggaa
    19621 gtcggcgccg ggaatggcca gcagcacgcc ggtcatgccg ccgatcacga aggtgatgat
    19681 gaagccgagc gtccagtaga cgggcgccgt catctgcacg cggccgcggt acatggtgaa
    19741 cagccagttg aagatcttca cgcccgtggg aatggagatg atcatcgtgg cgatgccgaa
    19801 gaaggaattg acgtcggcgc cggaacccat ggtgaagaag tggtgcagcc acacgacgaa
    19861 cgacatcacg ccgatcgccg cggtcgcgta gaccatggcc ttgtagccga acagcggctt
    19921 gcccgagtac gtggcgatga tttccgagaa cgcgccaaag acgggcagga ccaggatgta
    19981 gacctcgggg tggccccaga tccagatcag gttgatgtac atcatggcgt tgccgccgcc
    20041 gtcattggtg aagaagtgcg tgcccaggta gcggtcggcc gtcagcagcg ccagggtggc
    20101 ggtcagcacg gggaaggcgg cgacgatcag cacgttggtg accagcgcgg tccaggtgaa
    20161 cacgggcatc tgcatcagct tcatgccggg cgtgcgcatg cgcaggatgg tcacgatgaa
    20221 gttgatgccg gacagcgtcg ttcccaggcc ggatatctgc aaggcccaga tgtagtagtc
    20281 cacccccacg ctcgggctgt agtccaggcc cgacagcggc ggataggcca gccagccggt
    20341 ggccgcgaat tcgccgacga acagcgacag catgaccagg atcacgccgg cgacgaacag
    20401 ccagaaggac agcgagttca ggaagggata ggccacgtcg cgcgcgccga tctgcagcgg
    20461 cacgaccacg ttcatcaggc ccacgatgaa aggcatggcc atgaagaaga tcatgatcac
    20521 gccgtgggcg gtgaacacct ggtcgtagtg gtgcggcggc aggaagccgg gcgagtcgcc
    20581 gccggccacg gccagctgcg tgcgcatcat gatggcgtcg gcgaagccgc gcagcagcat
    20641 gatcagcgcc acgaagatgt acatgacgcc gattttcttg tggtcgaccg aggtgagcca
    20701 gtcggtccac aagggtttcc agaggcggaa gtaagtcatg gccgccagca cgacgatagc
    20761 gccgaacatc acgccagcca gcgtcaccat cacgatgggc tcgtggtagg gaacggattc
    20821 cagggaaagt ttgccgaaca tcatttactc cgcgatggcg accaggttct tggacgtggg
    20881 ggtgcagatg gaagaatcca caccggcgat cttggccatc tggctatgca ggatgaactc
    20941 gaacatgctg ggcggcgtgc tcgagtagaa cgcgaccgga ttttgct
//
LOCUS       DASWPO010000268        20066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3008678, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000268 DASWPO010000000
VERSION     DASWPO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20066
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008678"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGC69_14090"
     CDS             <1..206
                     /locus_tag="VGC69_14090"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY0296456.1"
                     /translation="TGWPSFWQPLDNTVGTTTDRSFGVVRTAAHCRRCGGHLGHVFKD
                     GPPPTGLRYCMNGLAMTFATRPA"
     gene            244..2052
                     /locus_tag="VGC69_14095"
     CDS             244..2052
                     /locus_tag="VGC69_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEY0296457.1"
                     /translation="MLLLILAYLGGVLTILSPCILPVLPFVFSRAGLSFTRSGLPLLA
                     GMALTFAVAASLAAVGGGWVVAANQYGRWLALVLVALFALTLLWPRLAERLTHPLVSA
                     GNRLTEAAQAGGPRPGASFLLGIATGLLWAPCAGPILGLVLTGAALQGASLSTTLLLL
                     AYAAGAATSLAVALLIGGRVYAAMKRSLGAGEWLRRALGVAMLAGVGAIALGLDTSVL
                     AQVSLASTGGLEQKLVDQLSSPRNGNAAMQGAPAMMSATADGAGPAMMAAGPAMRAAA
                     PAEPELPVEGALPPLDGAVQWLNSPPLTAAGLRGKVLLVDFWTYSCINCLRALPYVKA
                     WADKYRDQGLVVIGVHSPEFAFERDADNVARAVKRYGLDYPVAIDNNYAIWRAFGNQY
                     WPAHYFIDAQGRIRHHHFGEGDYAESEHVIQQLLREAGATQVTDTVADVHAQGDQLAA
                     DMNDVQSPETYLGYERADRFASGPAARDSVHAYAAPAMPALNAWGLDGDWTVGPEQAS
                     LSGSAGRIVYRFHARDLHLVLGPGAGGKPVRFKVTLDGQAPGNAHGDDVAPDGTGVVT
                     GQRLYQLVRQSGQVGDHTFSIEFLDPGVSAYAFTFG"
     gene            2099..2845
                     /gene="msrA"
                     /locus_tag="VGC69_14100"
     CDS             2099..2845
                     /gene="msrA"
                     /locus_tag="VGC69_14100"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0296458.1"
                     /translation="MTTTPSSSHPARRRALLVTLAGGAVLAAALAWQSPAWSFGGAES
                     AVSIAPPAQDETPNAPSETAVFAGGCFWGVQGVFQHVRGVQRVLSGYAGGSANTAQYE
                     TVSTGTTGHAESVQITYDPSQVSYGTLLQIFFSVAHNPTELNRQGPDSGTQYRSAIFP
                     ANAEQKRVAQAYIAQLDAAHAFPEPIATRIEDNKSFYPAEAYHQDFLTRHPTYPYIVI
                     NDMPKIGYLQHLFPARYSENPVLVDKAAAS"
     gene            2959..3714
                     /locus_tag="VGC69_14105"
     CDS             2959..3714
                     /locus_tag="VGC69_14105"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY0296459.1"
                     /translation="MKSTLRQRLRPLLALPLLVFLAHAHAQDSAGTHWGLGAGAGYRE
                     VPFNGGGARFGALPYFFFDNSWLHAEGTTLDLKLGSTHGLDFTLRGKYALEDRRTDSL
                     AASLNGMDQRHGAFQIGPTVRWHSDLGIASAAFLVAGDKGQQLVLDYTKPFQYGAWTL
                     SPYVGVRWLSDSYVDYYYGVRSSEARPGRPAYDGNSTYELSLGSRFRYQFTPHQSVMF
                     DLGVARLGSGITDSPIVGRQYVPRASIGYLYQF"
     gene            3730..4419
                     /locus_tag="VGC69_14110"
     CDS             3730..4419
                     /locus_tag="VGC69_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0296460.1"
                     /translation="MSRVALIEDHERLAQMIRQGLSASGIEADLFGRISEAAYAVAHA
                     DYAVLIMDRGLPDGDGLAFLRTLRAQGHAVPCLMLTARDALHDRVDGLESGADDYLTK
                     PFAMSELVARVRTLMRRPAALAARMPALGDIAVDPAQQTVQCGSQSSRLAPAELQILL
                     ALIRAEGRTVRHAALEHAAWGFSDSVTPNALDVTLHRLRKKLVALGATTRLVNTRGTG
                     FALREMDAPDA"
     gene            4412..5785
                     /locus_tag="VGC69_14115"
     CDS             4412..5785
                     /locus_tag="VGC69_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0296461.1"
                     /translation="MLENWFRKLSVQVWATSVAVLAVILAALVILTIYSFDHPTGARD
                     SREQFNRAQNLASNLQYDADGQPVDIRIPDPDGIMAWIFRVIPDEVKYRVLDERGESL
                     LSSNKTDASGPWIPGDPEAALDQLRRVTIGPTTYNIMTARTQHAGRIFYVQIAASDMF
                     NRAIVHQKTQDLPGVVRGTFIVVMLVFALTLSLTVRRVLKPLRKVSDAAASITPRNLA
                     QRLPTQSVPSEIRPLIHAFNATLDRIEHGYATQQQFLSAAAHELQTPLTLLRGQIELA
                     GDIAGKDLLLREIDFMARQVRQLLHLAEVSEPESFAFAEINAAAVARDVLSYLDGKAR
                     SSQVRLALDAPGDTVPLWADRSALFILLKNIVENAINASPPGGEVNVAVEPDAIRVED
                     DGPGISEADLPMLFQRFRRGAGARHPGAGLGLSICREIALAHQWRLAVAPRTPGTRFI
                     VWTHKPA"
     gene            5877..7445
                     /locus_tag="VGC69_14120"
     CDS             5877..7445
                     /locus_tag="VGC69_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY0296462.1"
                     /translation="MRHPLTALGLALALTFPLAANTRAQTAPTPAPGAQAAAAKAPPR
                     DPFFWLGEINKASAVINTDEGLLDKSMAPRLAAGVAKVIHDGNQPGGKRPSSVITFEP
                     LMIQAAGEDVTLLHAGRSSQDMHATFRSAILRDKLLDLADQLNATATTLVNLADKHVD
                     TIVPNYTNGVAAQPNSYGHYLLGYAAALDRDAQRIRETYARVDRSAMGTTVLNGTSWP
                     LDRTRMAQYLGFAALVDNAYDASQISSMDEPVEVASIITSIALHIGNFVQDTMTQYAE
                     SRPWILLQEGGGNTYVSSAMPQKRNPGLLNDTRSDASTALTLAMGPVIQTHNLTPGMP
                     DPKDVKQNSAMVDSAINALKRWDRALKALVINPDRALEELNSDWTASQELADVLMRKY
                     KVPFRVGHHFASDVVEYARAHDIKPLDFPYDQSKRIYEDVVKDTKYPPELPMSEDEFR
                     ATLNPIAIVKNRATIGGPQPAEMARMLKEARQRLAAQDAWIKAHRAHINDSLADLDKR
                     FDALVASAGDVKTQ"
     gene            7569..8321
                     /locus_tag="VGC69_14125"
     CDS             7569..8321
                     /locus_tag="VGC69_14125"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HEY0296463.1"
                     /translation="MGFEQLAALRDQLAAERAAREGHTKTAPAKTAKSDDKKPRGPKP
                     AQARQDGQPAGKPQRAPGAARKGQGQGQRRQAPQARPQPETPRDPLLVAIGRLQQAFP
                     KAFPKKPDPRVALKLGIVEDLYVHAAKLRLSEDDIKAAIAAWCSVGRYWAALTKDAVR
                     VDINGEPSGTVTAAEAGHASFLARQQRKKAQAAAKAAAPTDTPTDTLVDTPAAAPAGE
                     APPQAAATPDAAPALAESPAAVDAPAGDTASA"
     gene            8522..9544
                     /locus_tag="VGC69_14130"
     CDS             8522..9544
                     /locus_tag="VGC69_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296464.1"
                     /translation="MRPTLPRLLPAALLLAAGAALCLHGAAAQAEGRIRVAQQFGIGY
                     LILDVVRDQQLIEKHGKQEGLDIAVEWAQISGSTAMDEALLSNNLDVVSAGVPPALVL
                     WDRTRGRQNVKLIATLGSLPNYLLTNNPAIQTLDDFSDKDRIAVPAAGAGFQSRTLQI
                     EAARRWGKANFKKLDDISVSLPHPDATAALISGGLEVDAHFSSAPFYYQALAANPKIH
                     KVISSYDILGGPASFNALYTTQKFHDENPKTYKAFFDALVEAEQWIAGHKAEATGVFI
                     RVQHSKLPPDLIRAIIDDPENQFTIAPQRTKVYADEMYKLGVLKNQAQSWQDYFFDEA
                     HALPGS"
     gene            complement(9634..10407)
                     /gene="pstB"
                     /locus_tag="VGC69_14135"
     CDS             complement(9634..10407)
                     /gene="pstB"
                     /locus_tag="VGC69_14135"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY0296465.1"
                     /translation="MDTQSQVSAKLEVKNLNFYYGHFHAIRNVNMSIRENKVTAFIGP
                     SGCGKSTLLRTFNRMFELYPGQRAEGEIILDGENLLTTDTDISLIRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFERLSKGEMDERVEWALTKAALWKEVKDKLNQSGSGLSGGQQQ
                     RLCIARGVAIKPQVLLLDEPCSALDPISTAKIEELIAELKGEYTVVIVTHNMQQAARC
                     SDYTAYMYMGELMEFGETDQIFVKPKRKETEDYITGRFG"
     gene            complement(10460..11317)
                     /gene="pstA"
                     /locus_tag="VGC69_14140"
     CDS             complement(10460..11317)
                     /gene="pstA"
                     /locus_tag="VGC69_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY0296466.1"
                     /translation="MSVLNMSNSIYRRRRMVNKVMLTLSFAALLFGLFWLFWIIATLL
                     YKGAAGLTPSMFTQITPPPGSDGGLINSIVGSVLMSGLGTLIGTPVGILAGTYLAEYG
                     RRGWLAPATRFINDVLLSAPSIVVGLFIYTVYVLQVRHYSGWAGSLALSVLVIPVVVR
                     TTDDMLKLVPNSLREACAALGCPEWKMVTLVCYRAARSGIVTGVLLAVARIVGETAPL
                     VFTALNSQFMTWNMNAPMSNLPMTIFQYAMSPYEDWQRLAWAGATLITLLVLVVNIVA
                     RSMFSKPNL"
     gene            complement(11322..12263)
                     /gene="pstC"
                     /locus_tag="VGC69_14145"
     CDS             complement(11322..12263)
                     /gene="pstC"
                     /locus_tag="VGC69_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0296467.1"
                     /translation="MEKPKRPFADILFRNATRFFAFGVLCLLLAILISLVYGSSVTLH
                     KYGLSFIWTNEWDPVNSEFGALVPIIGTLGTSIIALLIAVPVSFGIAMFLTELSPTWL
                     RRPLGIAIEMLATIPSIIYGMWGLFIFAPIFQRYIEPFLISALSPIPVIGALFKGPAF
                     GIGIFTAGGILAVMIIPFITAVMRDVFELVPPLLKESAYGLGGTTWEVMSKVVLPFTK
                     VGVIGGIMLGLGRALGETMAVTFVIGNAFRLSGSLFAPGNSIASALANQFAEASDPTH
                     LSALIELGLILFLITTIVLAFSKLLLLRLAKSEGTRS"
     gene            complement(12438..13484)
                     /gene="pstS"
                     /locus_tag="VGC69_14150"
     CDS             complement(12438..13484)
                     /gene="pstS"
                     /locus_tag="VGC69_14150"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY0296468.1"
                     /translation="MFMRVFKQATLGAALSAAVFAAHAADITGAGSSFAFPVYSKWAT
                     GYKAAAGATVNYQSVGSGAGIKQIEAKTVDFGASDVALSGEDLDKNGLIQFPGVVGGI
                     VPVVNLAGVAPGQLKFTGSLLADIYMGKITKWNDAAIAKLNPGVKLPSSDIIPIYRSD
                     ASGTSYNYTAYLSDVSADWKSKLGFGTDVKWPVGQGGKGNEGVANLVKQFQGAIGYVE
                     IAYSTQNKLTYGLVANKAGKYVEPNLASFTAAASNANWDAQPGMGVVLVDEPGDQSWP
                     LTVATYVLVQKVQDKPASGAEVLKFFDYGYTQGKKDAEDLVYVPLPDALVTKIRAVWA
                     QVKDASGKPVYAGK"
     gene            complement(13758..15884)
                     /gene="ppk1"
                     /locus_tag="VGC69_14155"
     CDS             complement(13758..15884)
                     /gene="ppk1"
                     /locus_tag="VGC69_14155"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0296469.1"
                     /translation="MKQRELEKTIQESAAAARAGGEPRLFNRELSLLKFNERVLAMAE
                     HPSVPILERLRYVCIVSANLDEFFEIRISSLKEQQRQTPGLVAPDGMTPDEAFDRVQA
                     KVHSLVEHQYRVLNEHILPSLQAEGVVLHHASEWDEAQQQWAHEVFTREVMPLLTPIG
                     LDPAHPFPRVYNKSLNFIVSLSGADAFGRQASIAVVQAPRALPRLIRMPAELSGHPDG
                     YILLTSLLRAFVGELFPGLTMHGCYQWRVTRNSDLFVDEEEVTNLRLALQGELSQRNF
                     GSAVRLEIDWIMPPELEQYLQREFSLTPADTYRVPGPVNLSRLMQLCNTNTRPDLLFS
                     LYLAPVPAPFDKLRGKPAELFAAVAERDRLLHHPYQSFQPVIDFLTAAAIDPDVMAIK
                     QTIYRTGEDSELMQILLAAARAGKEVTVVVELMARFDEQTNMNWAARLEEVGAHVVYG
                     VVAHKTHAKMALVLRREDGRLRRYAHLGTGNYHPRTARLYTDFGLLTADPRLCEDMDK
                     VFGQLTGLGARRELKSLLQSPFTLHAGMVALIRAEAEAARAGRKARIMAKMNSLLEAQ
                     VIEELYLASQAGVKIDLIVRGVCALRAGVKGLSENIRVRSVVGRFLEHSRVFYFHADG
                     EETVYLSSADWMDRNFFRRVEVAFPVYDRALKKRVIAEAFTYALRDNQLAWQLQADGT
                     YVRVVNRRPPFGLHDYLMEKLGQDGG"
     gene            16072..17562
                     /gene="ppx"
                     /locus_tag="VGC69_14160"
     CDS             16072..17562
                     /gene="ppx"
                     /locus_tag="VGC69_14160"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY0296470.1"
                     /translation="MDHLLAAVDLGSNSFRLSIGRVVQQNGMAQIYQIDRLKETVRLA
                     AGLGTDKQLDGSAIERAIAVLERFGERLRSFHPNRVRAVATNTFRVARNTTEFLPDAE
                     AALGFPIDVIAGREEARLIYLGVMHSLPPSPDRRLVIDIGGGSTEVIIGKGFEPGLMS
                     SLYMGCVSYSRQFFGDGLVDAHRMKQAEMAARREVEVISKQYRKMGWNAAFGSSGTAK
                     ALYAILTECGFSTAGITRAGMNKLKERIIRSGRVIPEELPGIKLDRADVLPGGLAIMS
                     AIFDELNIDAMQTGDGALRLGVLYDLLGRDDAHDKRDESVRQFMKRYHVDAQQARRVR
                     ETALAFFDSILPEDQEEHADLRRALGWAADLHEVGLSIAHNGYHRHSAYVLQNADMPG
                     FSQADQQSLALLTLAHQGKLGKVEPLVRSRAQWLAILSLRLAVLLFRRRQDISPLPLK
                     VSVKGNSIVVKVDAGWLESHPLSDFTLRAEDSEWRKVGFAFELIER"
     gene            complement(17705..18496)
                     /locus_tag="VGC69_14165"
     CDS             complement(17705..18496)
                     /locus_tag="VGC69_14165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEY0296471.1"
                     /translation="MLELARIDAPSEGAQAWPGTHSRGLAVGLARSQEEVEQIQRLRY
                     TVFTEDMGAVFPDAEDGIDQDRFDPWCEHLMVRELDTNRVVGTYRILTPQKAREAGGY
                     YSESEFDLSGLNDLRDQLVEVGRSCTHANYRNGTVIMLLWSGLAEFLRRGGYHYLLGC
                     ASVSLRDDGVTAAEVWRTVSKHLQDPDAPRVKPLHRYPVERLNSTLPARVPPLIKGYL
                     KLGAKVCGEPAWDPDFNAADFPVLLTTDRMDDRYRRHFGLERIER"
     gene            complement(18655..20001)
                     /gene="glmM"
                     /locus_tag="VGC69_14170"
     CDS             complement(18655..20001)
                     /gene="glmM"
                     /locus_tag="VGC69_14170"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0296472.1"
                     /translation="MSQQRKYFGTDGVRGEVGGPVINAGFALHLGYAAGKVLSRAHGK
                     RPGRPQLLIGKDTRISGYMLESALEAGLAAAGVDTLLAGPIPTPAVAYLTRALRLTAG
                     IVISASHNPYQDNGIKFFSAEGMKLPDEIELQIEAELGNPLGCVTSEELGRARRLDDA
                     QGRYIEFCKSTFPNDLDLSGMRLVIDAAHGAAYNTAPHVFRELGAEVRVIGASPDGLN
                     INKGVGALHPEALVAEVKAYRADLGIALDGDADRLQMVDATGRVYDGDELLYAIVRSR
                     MAAGPVGGVVGTLMTNYGLELRLKELGIPFERAKVGDRYVLEMLLERGWLYGGENSGH
                     LICLDRHTTGDGTIAALQVLTALRRGGMPLAEWVGDLRMFPQVMINVPLKPGQDWQSH
                     AGLAAARSTVEADLAGKGRVLIRASGTEPKLRLMVEAEDERLAEASAHKLAASLAA"
BASE COUNT         3489 a         6806 c         6536 g         3235 t
ORIGIN      
        1 gcaccggctg gccgagcttc tggcagccgc tggacaacac ggtcggcacg accaccgacc
       61 gcagcttcgg cgtcgtgcgc acggcggcgc actgccggcg ctgcggcggc cacctgggcc
      121 acgtgttcaa ggacggcccg ccgcccaccg gcctgcgcta ttgcatgaac ggcctggcca
      181 tgactttcgc gacgcggccg gcctgacgcc gcgcggcccc tcctcccctt ccggacgtat
      241 tccatgctgc tactcatcct tgcctatctc ggcggcgtac tgaccattct cagtccgtgc
      301 atcctgccgg tcctgccgtt cgtgttctcg cgtgccggac tctcctttac gcgcagcggc
      361 ctgcccctgc tggccggcat ggcgctgacc ttcgcggtag ccgcctcgct ggccgccgtg
      421 ggcggcggct gggtggtggc ggccaatcaa tacggccgtt ggctggcgct ggtgctcgtg
      481 gcgctgttcg cgctgacgct gctctggccg cgcctggccg agcgcctgac ccatccgctg
      541 gtgtcggccg gcaaccgcct gaccgaagcg gcgcaggccg gcggcccgcg gcccggcgcc
      601 tcgttcctgc tgggcatcgc caccggcctg ctgtgggcgc cgtgcgccgg ccccatcctg
      661 ggcctggtgc tgaccggcgc cgcgctgcag ggcgccagcc tgagcaccac gctgctgttg
      721 ctggcctacg cggccggcgc ggccacctcg ctggccgtcg cgctgctgat cggcggcagg
      781 gtctacgcgg cgatgaagcg ctcgctgggc gccggcgagt ggctgcggcg cgcgctgggc
      841 gtggccatgc tggccggcgt gggcgccatc gcgctgggcc tggacacgag cgtgctggcc
      901 caggtgtcgc tcgcctcgac cggcgggctg gaacaaaagc tggttgacca attgtccagt
      961 ccgcgcaacg gcaacgccgc catgcagggg gcgcccgcca tgatgagcgc cacggccgac
     1021 ggcgccggac cggccatgat ggcggccggc ccggccatgc gcgcggccgc gcccgccgag
     1081 cccgagctgc ccgtcgaggg cgcgctgccg ccgctcgacg gcgcggtgca atggctgaac
     1141 tcgccgccgc tgacggccgc gggactgcgc ggcaaggtgc tgctggtcga cttctggacc
     1201 tactcgtgca tcaactgcct gcgcgccctg ccctacgtga aagcctgggc cgacaaatac
     1261 cgcgaccagg gcctggtggt gatcggcgtg cactcgcccg aattcgcctt cgagcgcgac
     1321 gccgacaacg tcgcccgcgc cgtcaagcgc tacggcctgg actacccggt ggccatcgac
     1381 aacaactacg cgatctggcg cgccttcggc aaccagtact ggccggcgca ctatttcatc
     1441 gacgcgcagg gccgcatccg ccaccaccac ttcggcgagg gcgactacgc cgaatcggag
     1501 cacgtcatcc agcagctgct gcgcgaggcc ggcgccaccc aggtgaccga caccgtggcc
     1561 gatgtccatg cccagggcga ccagctggcg gccgacatga acgacgtgca gtcgcccgag
     1621 acctacctgg gctatgaacg cgccgatcgc ttcgcttcgg gcccggcggc gcgcgacagc
     1681 gtccacgcct atgcggcccc cgccatgccc gcgctgaacg cctggggcct ggacggcgac
     1741 tggacggtcg gtcccgagca ggccagcctg tcgggctcgg cgggacgcat cgtctaccgt
     1801 ttccatgcgc gcgacctgca cctggtgctc ggccccggcg cgggcggcaa gccggtacga
     1861 ttcaaggtca ccctggacgg ccaggccccg ggcaacgccc atggcgacga cgtcgcgccc
     1921 gacggcaccg gcgtcgtgac cggacagcgc ctgtatcaac tggtccggca atccgggcag
     1981 gttggcgacc atactttcag catcgaattc ctcgaccccg gggtgtcggc ctatgccttc
     2041 accttcggtt gacccacggc acaccccttc cgcgacactg caccaggagc ttcgcatcat
     2101 gacgacaacg ccttcttctt cccatccggc gcgccggcgc gccctgctcg tcacgctggc
     2161 cggcggcgcg gtactggccg ccgccctggc ctggcaaagc ccggcatggt ccttcggcgg
     2221 cgccgaatcg gccgtgtcca tcgccccgcc ggcgcaggac gagaccccga atgcgccttc
     2281 cgagaccgcc gtgttcgccg gcggctgctt ctggggcgtg caaggcgtgt tccagcacgt
     2341 gcgcggcgtg cagcgcgtgc tgtcgggcta cgccggcggt tcggccaata cggcgcaata
     2401 cgaaaccgtc agcaccggca cgaccggcca tgccgaatcg gtgcagatca cctacgaccc
     2461 gtcgcaagtg tcatacggca cgctgctgca gatcttcttc tcggtggcgc acaaccccac
     2521 cgagctcaac cgccaggggc cggacagcgg cacgcagtac cgctcggcga tctttcccgc
     2581 caatgccgaa cagaaacgcg tggcgcaggc ctacatcgcg cagctcgacg ccgcgcatgc
     2641 cttccccgag cccatcgcca cgcgcatcga ggacaacaag tcgttctacc cggccgaggc
     2701 ctaccaccag gacttcctga cccggcaccc gacgtatccg tacatcgtca tcaacgacat
     2761 gccgaaaatc ggctacctcc agcacctgtt cccggcccgc tacagcgaga acccggtgct
     2821 ggtggacaag gctgccgcct cgtaggcggg gcgccgccct ggcctggcca cgccaggcca
     2881 aaaatcctcc acgggtaagt gaccggtaag tctgccgctt ctacagtctg cgcactttca
     2941 ttttctacga ggatcctcgt gaaatcgaca ttgcgccaac ggctgcggcc gctgcttgct
     3001 ttacccttgc tggttttcct ggcccacgcc catgcccagg actcggccgg cacgcattgg
     3061 ggcctgggcg ccggggcggg ctaccgggaa gtgcccttca atggcggcgg cgcccgcttc
     3121 ggggcattgc cctatttctt tttcgacaat agctggctgc atgccgaagg cacgacgctg
     3181 gacctgaaac tgggcagcac ccatggcctg gatttcacgc tgcgcggcaa atacgccctg
     3241 gaagaccggc gcaccgactc cctggccgcc tcgctcaacg gcatggatca gcgccacggc
     3301 gcattccaga tcggaccgac ggtacgctgg cattcggacc tgggcatcgc gtcggccgcg
     3361 ttcctcgtgg ccggcgacaa ggggcagcaa ctcgtcctgg actacacgaa gcccttccaa
     3421 tatggcgcgt ggacgctctc gccctatgtc ggggtccgct ggctgagcga cagctacgtg
     3481 gactactatt acggcgtccg gtcgtcggag gcccggcccg gccggcccgc ttacgacggc
     3541 aattcgacct acgaactgtc gctgggctcc aggttccgct atcaattcac accccatcaa
     3601 agcgtcatgt tcgaccttgg cgtcgcccga ctgggcagcg gcatcacgga tagccccatc
     3661 gtgggcaggc agtatgtccc gcgcgcttcg atcggctatc tctatcagtt ctgaccattc
     3721 cccttcgcca tgtcccgggt cgccctgatc gaagaccatg aacgcctggc gcagatgatc
     3781 cgccagggcc tgtcggcctc gggcatcgag gccgacctgt tcggccggat ctccgaagcc
     3841 gcctacgccg tggcccatgc cgattatgca gtgctcatca tggaccgcgg cctgcccgac
     3901 ggcgacggcc tggctttcct gcgcacgctg cgcgcccagg gccacgccgt tccctgcctg
     3961 atgctgaccg cgcgcgatgc gctacacgac cgcgtcgacg gcctggaaag cggcgcggac
     4021 gactacctga ccaagccttt cgcgatgagc gaactggtgg ctcgcgtgcg cacgctcatg
     4081 cgccggcccg ccgcgctggc ggcccgcatg cctgcgttgg gcgatatcgc ggtcgacccc
     4141 gcccagcaaa ccgtgcagtg cggctcgcag tcgtcgcggc tggcccccgc cgagctgcag
     4201 atcctgctgg cgctgatccg ggccgaagga cgcaccgtgc ggcatgccgc cctggaacac
     4261 gcggcctggg ggttcagcga cagcgtcacg ccgaacgccc tggacgtcac gctgcacagg
     4321 ctgcgcaaga aattggtggc gcttggcgcc acgacccggc tggtcaatac gcgaggcacc
     4381 ggcttcgccc tacgcgagat ggacgccccc gatgcttgag aactggttcc gcaaactgtc
     4441 ggtgcaagtc tgggcaacca gcgtggcggt gctggccgtg atcctggcgg cgctggtgat
     4501 actgacgatc tatagcttcg accacccgac gggcgctcgc gacagccgcg aacagttcaa
     4561 ccgtgcccag aacctggcct cgaacctgca atacgacgca gacggccaac cggtcgacat
     4621 acgcatcccc gaccccgacg gcatcatggc ctggatcttt cgggtcattc cggacgaggt
     4681 gaaataccgc gtgctggatg agcggggcga gtccttgctg tcctccaaca agacagacgc
     4741 ctccggcccc tggattccgg gcgacccgga ggcggcgctc gaccaactgc ggcgggtaac
     4801 gatcgggccg acgacgtaca acatcatgac ggcacgcacg cagcatgccg gccgtatttt
     4861 ctacgtgcag atcgccgcca gcgatatgtt caaccgcgca atcgtgcacc agaagacgca
     4921 ggaccttccc ggcgtggtcc gcggcacctt catcgtcgtc atgctcgttt tcgcgctcac
     4981 gctgagtctg accgtgcggc gcgtcctcaa gccgctgcgc aaagtgtccg acgccgccgc
     5041 gtcgatcacg ccgcgcaacc ttgcccagcg cctgccgacg cagagcgtgc ccagcgagat
     5101 ccgccccttg atccatgcct tcaatgcgac gctggaccgg atcgagcatg gctacgccac
     5161 gcagcagcaa ttcctgagcg ccgccgccca cgagttgcaa acgccgctta ccctgctgcg
     5221 cggccagatc gaacttgccg gcgacatcgc cggcaaggac ctgctgctgc gggaaatcga
     5281 cttcatggcc cgccaggtcc gccaactgct gcacctggcc gaagtcagcg aaccggaaag
     5341 cttcgccttt gccgagatca atgccgcggc ggtcgcacgc gatgtgctgt cctacctgga
     5401 cggcaaggcc agaagctcgc aggtacgcct cgctctggat gcccccggcg acaccgtgcc
     5461 cctctgggcc gataggagcg cattgttcat cctgctcaag aacatcgtgg aaaacgccat
     5521 caacgcctcg ccgccaggcg gcgaagtcaa tgtcgcggtc gagcccgatg cgatccgggt
     5581 cgaagacgac ggacccggca tttcggaagc ggacttgccc atgctgttcc agagattccg
     5641 gcgcggcgcc ggcgccaggc atcccggcgc cggcctgggc ctttccatct gccgcgaaat
     5701 cgcgctggcg caccagtggc ggctggccgt ggccccgcgc acgcccggga cgcgtttcat
     5761 cgtctggacg cacaagccgg cctgacccca tcggcccggg gatgtcgcgc gcccgcccaa
     5821 acggcggccg ccccgataaa ctatgccttt tccggcataa cgataacgat cccatcatga
     5881 gacatcccct caccgccctg ggcctggcgc tggccctgac ctttcccctt gcggcgaaca
     5941 cccgggccca gaccgcgccg accccggcgc ccggcgcgca ggccgccgcc gccaaggccc
     6001 cgccgcgcga cccgttcttc tggctgggcg agatcaacaa ggcctccgcc gtcatcaaca
     6061 ccgacgaagg cctgctggac aagtccatgg cgccgcgcct ggccgccggc gtggccaagg
     6121 tgatccacga cggcaaccag cccggcggca agcgcccgtc ctccgtcatc accttcgaac
     6181 ccctgatgat ccaggccgcg ggcgaggacg tcacgctgct gcatgccggc cgctccagcc
     6241 aggacatgca cgccaccttc cgcagcgcca tcctgcgcga caagctgctg gacctggccg
     6301 accagctcaa cgccaccgcc accacgctgg tgaacctggc cgacaagcac gtcgacacca
     6361 tcgtgcccaa ctacaccaac ggcgtggcgg cgcagcccaa cagctacggc cactatctgc
     6421 tgggttatgc cgcggcgctg gaccgcgacg cgcaacgcat ccgcgagacc tatgcgcgcg
     6481 tcgaccgcag cgccatgggc accacggtgc tcaacggcac cagctggccg ctggaccgca
     6541 cgcgcatggc ccaatacctg ggctttgccg ccctggtcga caacgcctac gacgcctcgc
     6601 agatctcgtc catggacgag ccggtggaag tcgcgtccat catcaccagc atcgcgctgc
     6661 acatcggcaa cttcgtccag gacaccatga cgcagtacgc cgaatcgcgc ccctggatcc
     6721 tgctgcagga aggcggcggc aatacctatg tgtccagcgc catgccgcaa aagcgcaacc
     6781 cgggcctgct caacgacacc cgcagcgacg cttccacggc gctcacgctg gccatgggcc
     6841 cggtgatcca gacgcacaac ctcaccccgg gcatgcccga ccccaaggac gtcaagcaga
     6901 actccgccat ggtcgacagc gccatcaacg cgctcaagcg ctgggaccgc gccctcaagg
     6961 ccctggtcat caacccggac cgcgccctgg aagaactcaa cagcgactgg acggcctcgc
     7021 aggagctggc cgacgtgctg atgcgcaaat acaaggtgcc tttccgcgtc ggccatcact
     7081 tcgcctccga cgtggtcgaa tacgccaggg cgcacgacat caagccgctg gacttcccct
     7141 atgaccagtc caagcggatc tacgaagacg tggtcaagga cacgaaatac ccgcccgagc
     7201 tgccgatgag cgaggacgag ttccgcgcga cgctgaaccc gatcgccatc gtcaagaacc
     7261 gggccaccat cggcgggccg cagcccgcgg aaatggcgcg gatgctcaag gaagcgcgcc
     7321 aacgcctggc cgcgcaggac gcctggatca aggcccaccg ggcgcacatc aacgactcgc
     7381 tggccgacct ggacaagcgc tttgacgcgc tggtggccag cgccggtgat gtaaaaacgc
     7441 aatagtcgca tccgtccccg ggcaggccaa ggggccttgc ccggcgcccc agtcgcgggg
     7501 cgcatacaat aagcccacta aaaaacacaa ccgccgacca ggcgacgttt caccaaagcg
     7561 acagacccat gggattcgaa caactcgccg ccctgcgcga ccagctggca gccgagcgcg
     7621 ccgcgcgcga aggccacacc aagacagcac cggccaagac cgccaagagc gatgacaaaa
     7681 agccgcgcgg ccccaagccg gcccaggccc gccaggacgg ccaaccggcc ggcaagccgc
     7741 agcgcgcacc gggggcggcc cgcaagggcc agggccaagg ccagcgccgc caggcccccc
     7801 aggccaggcc gcaaccggaa accccgcgcg acccgctgct ggtggccatc ggccgcctgc
     7861 agcaggcctt tcccaaggcc tttcccaaga agccggaccc gcgggtggcg ctcaagctgg
     7921 gcatcgtgga agacctctac gtccatgccg ccaagctgcg cctgagcgaa gacgacatca
     7981 aggcagccat tgccgcctgg tgcagcgtgg gccgctactg ggccgcgctc accaaggatg
     8041 ccgtgcgggt cgacatcaac ggcgagccca gcggcacggt gacggctgcc gaggccggcc
     8101 acgccagctt tttggcgcgc cagcagcgca agaaggccca ggctgccgcc aaggcggccg
     8161 cgccgactga tacgccgact gatactctgg tcgatacgcc ggcggctgcg ccggccggcg
     8221 aggcgccccc ccaggcagcg gccacgccgg atgccgcgcc cgccctggcg gaaagccccg
     8281 ccgcggtcga tgcgccggcc ggcgatacgg ccagcgcctg acgctgacga agggctgtac
     8341 cggggcattc cccgtacagc cggccagcct ctggcccatg ccggaatagg ccagtctttc
     8401 gcttctgtgc aaagggcatt tgcacagaag cagaagttcg ttcgcgtcgc ctgcgtagct
     8461 gcctagaatc tgcggtcttg ccgacccctt ttcttgccgc ccgccacgga gacacccgcc
     8521 catgcgtcct accctgccac gcctcttgcc tgccgcgctc ctgctggccg cgggcgctgc
     8581 cctttgcctg cacggcgccg ccgcccaggc cgagggccgc atccgggtgg cgcagcaatt
     8641 cggcatcggc tatctgatcc tggacgtggt gcgcgaccag caattgatcg agaagcacgg
     8701 caagcaagag ggcctggaca tagccgtgga gtgggcgcag atctccggct cgaccgccat
     8761 ggacgaggcg ctgctgagca acaacctgga cgtcgtgtcg gccggcgtgc cgcccgccct
     8821 ggtgctgtgg gaccgcaccc gcggccggca gaacgtcaaa ttgatcgcca cgctgggttc
     8881 gctgcccaat tacctgctga ccaacaaccc cgcgatccag acgctggacg acttcagcga
     8941 caaggaccgc atcgccgtgc cggccgcggg cgcgggcttt cagtcgcgca cgctgcagat
     9001 cgaggcggcg cgccgctggg gcaaggccaa cttcaagaag ctggacgata tttcagtgag
     9061 cctgccgcat cccgacgcca cggccgcgct gatctccggc ggcctggaag tcgatgccca
     9121 cttctccagc gcgccgttct actaccaggc gctggcggcc aatccgaaga tacacaaggt
     9181 gatcagctcc tacgacatcc tgggcggacc ggcctcgttc aacgcgctct acaccaccca
     9241 gaagttccac gacgagaacc ccaagaccta caaggccttc ttcgacgcgc tggtcgaggc
     9301 cgaacaatgg atagccgggc acaaggccga ggccaccggc gtgttcatcc gcgtccagca
     9361 ctcgaagctc ccgccggact tgatccgcgc catcatcgac gacccggaaa accagttcac
     9421 catcgcgccg cagcgcacca aggtgtatgc cgacgagatg tacaagctgg gcgtgctgaa
     9481 aaaccaggcg cagtcgtggc aggactactt cttcgacgag gcgcacgcct tgcctggcag
     9541 ttgatcgccg cccaggccgc ctgacgccgt ccgccaatga aaacgcccgc gtggacgtcc
     9601 acgcgggcgt tttgtcgcag gcgcgagagc cggtcagccg aagcggccgg tgatgtagtc
     9661 ttcggtttcc ttgcgcttgg gcttgacgaa gatctggtcg gtctcgccga actccatcag
     9721 ctcgcccatg tacatatagg cggtgtagtc ggagcagcgc gcggcctgct gcatattgtg
     9781 cgtcacgatg accacggtgt actcgccctt gagctcggcg atgagttctt cgatcttggc
     9841 ggtggagatc gggtcgagcg ccgagcaggg ttcgtcgagc agcagcacct ggggcttgat
     9901 ggccacgccg cgggcgatgc acaggcgctg ttgctggccg cccgataggc cgctgccgct
     9961 ttggttcagc ttgtccttca cttccttcca caaggcggcc ttggtgagcg cccactcgac
    10021 gcgctcgtcc atctcgccct tgctgagccg ctcgaacagg cgcacgccga aggcgatgtt
    10081 gtcgtagatg ctcatgggga acggcgtggg cttctggaac accatgccca ccttggcgcg
    10141 gatcagcgag atgtcggtat cggtggtgag caggttctcg ccgtcgagga tgatctcgcc
    10201 ctcggcgcgc tggccaggat agagttcgaa catgcgattg aaggtgcgca gcagcgtgga
    10261 cttgccgcag ccggacggac cgatgaaggc cgtgaccttg ttctcgcgga tcgacatgtt
    10321 cacattacgg atcgcatgga agtggccgta atagaaattg aggttcttga cttccagctt
    10381 ggcggagact tggctttggg tgtccatttc ggttccggaa aataatggcg tcgtcggtgc
    10441 gggcgcgagg acggatatct cagaggttcg gcttgctgaa catcgaacgg gcaacgatgt
    10501 tgaccaccag caccagcagc gtgatcagcg tggcgccggc ccaggccagg cgctgccaat
    10561 cctcgtaggg actcatcgcg tactggaaga tggtcatggg caggttggac atcggggcgt
    10621 tcatgttcca ggtcatgaac tggctgttga gcgccgtgaa caccaggggc gcggtctcgc
    10681 cgacgatgcg ggcgacggcc agcagcacgc ccgtcacgat gcccgaacgg gcagcgcggt
    10741 agcagaccag ggtgaccatc ttccactcgg ggcaacccag ggcggcgcag gcttcgcgca
    10801 ggctgttggg caccagcttg agcatgtcgt cggtcgtgcg cacgaccacc ggaatcacca
    10861 gcacggacag cgccagcgaa ccggcccagc ccgagtagtg cctgacctgc agcacgtaga
    10921 cggtatagat gaacaggccg accacgatgg acggcgccga caacagcacg tcgttgatga
    10981 agcgcgtggc cggggccagc cagccgcggc ggccatactc agccaggtag gtgccggcca
    11041 ggatgccgac gggggtgccg atgagggtgc ccagccccga catcagcacg ctgccgacga
    11101 tggagttgat gagaccgccg tcggaacccg gcggcggcgt gatctgggtg aacatcgagg
    11161 gggtaagccc ggccgcgccc ttgtagagca gcgtggcgat gatccagaac agccagaaca
    11221 ggccgaacag cagcgcggcg aacgacaagg tgagcatcac cttgttgacc atgcggcggc
    11281 ggcggtagat cgaattactc atgttgagga cagacatggg atcagctccg cgtgccttcg
    11341 ctcttggcca ggcgcagcaa cagcagcttg gagaaggcca ggacgatggt ggtgatgagg
    11401 aacaggatca ggcccagttc gatcagcgcc gacaggtggg tgggatcgct ggcttcggcg
    11461 aactggttgg ccagcgccga ggcgatggag ttgcccggcg cgaacaggga gccggagagc
    11521 cggaaggcgt tgccgatgac gaaggtgacc gccatggttt cgcccagggc gcggcccagg
    11581 cccagcatga tgccgccgat gacgccgacc ttggtgaaag gcagcaccac cttggacatg
    11641 acttcccagg tcgttccgcc caggccgtag gcggactctt tcagcagggg gggcaccagt
    11701 tcgaacacgt cgcgcatcac ggccgtgatg aaagggatga tcatgacggc gagaatgccg
    11761 ccggcggtga atatgccgat gccgaacgcc ggccccttga acagcgcgcc gatgacgggg
    11821 atggggctca gggcgctgat gagaaaaggc tcgatgtagc gctggaatat cggggcgaag
    11881 atgaacaggc cccacatgcc gtaaatgatg gaaggaatgg tggccagcat ttcgatcgcg
    11941 atgccgagcg ggcggcgcag ccaggtcggc gacagctcgg tcaggaacat ggcgatgccg
    12001 aacgagaccg gcacggcaat gagcagcgcg atgatcgacg tgcccagcgt gccgatgatg
    12061 ggcaccaagg cgccgaactc gctgtttacc ggatcccatt cgttggtcca gataaacgac
    12121 aggccgtatt tgtgcagggt gaccgaactg ccgtacacca gcgatatcag aatcgccagc
    12181 agcaggcaca ggacaccaaa ggcgaagaaa cgcgtggcat tgcggaacag aatatccgcg
    12241 aatgggcgct tgggtttttc catggggggg attttcccgg cgagagagtc agcggggacg
    12301 ttcgcccgcg ttccgcccgg catattatca agtaccgcgc tcatcggctt ccctttctgg
    12361 aagacataaa acgaagaggg gcgagcgcag cgcccgcccc ctttactgct tatccgagat
    12421 atcccggaaa ccagcgctta cttaccggcg tagacaggct tgcccgacgc atccttgacc
    12481 tgggcccaga cggcgcggat cttggtgacc agggcatcgg gcagcggcac gtagaccagg
    12541 tcttcggcgt ccttcttgcc ctgggtgtag ccgtagtcga agaacttcag gacttcggcg
    12601 ccgctggccg gcttgtcctg caccttctgg accaggacgt aggtggcgac ggtcaggggc
    12661 cacgattggt cgccgggctc gtcgaccagc accacgccca tgccgggctg ggcatcccag
    12721 ttggcgttcg aggcggcggc ggtgaaggaa gccaggttgg gttcgacata cttgccggcc
    12781 ttgttggcga ccaggccgta cgtcagcttg ttctgggtgg agtaggcgat ttcgacgtag
    12841 ccgatggcgc cctggaactg cttgaccagg ttggccacgc cttcattgcc cttgccgcct
    12901 tggccgaccg gccacttcac gtcggtgccg aagccgagct tgctcttcca gtcggcggac
    12961 acgtcggaca ggtaggccgt gtagttgtac gaggtacccg aggcgtcgga acggtagatg
    13021 gggatgatgt cggagctggg cagcttgacg ccggggttca gcttggcgat ggcagcgtcg
    13081 ttccacttgg tgatcttgcc catgtagatg tcggccagca gggagccggt gaacttcagt
    13141 tggccaggcg cgacgcctgc caggttgacc acgggcacga tgccgccgac cacgccgggg
    13201 aactggatca gaccgttctt gtcgaggtct tcaccgctca gggcgacgtc ggaagcgccg
    13261 aaatcgacgg tcttggcttc gatctgcttg atgccggcgc ccgagccgac cgattggtag
    13321 ttcacggtgg cgcccgcggc ggccttgtag ccggtggccc acttggagta gacgggaaag
    13381 gcgaaggagg agcccgcgcc ggtgatgtcg gcggcatggg cggcgaaaac ggcggcgctc
    13441 agcgcggcgc cgagggtggc ttgcttgaaa acacgcataa acatcgtgga ttccttcggt
    13501 ggttgaaaga tgcgagcagt ttcaaatctg cttcaaggct tgcgcatctt agaagtaccg
    13561 agtgacagga ttatgacagt cacaatggag tttttgatga aaaacgtcta tatgattcaa
    13621 tacaagttat tgaaataaaa agaatcttta tttggggtgc gtcccttgta tttgatttac
    13681 aggctcaatt atgacaacgg gtttgcagcg ctcggcgtaa atattccggc agtgcggggc
    13741 aagcgccccc ggcaggctca gccgccatcc tgccccagtt tttccatcag gtaatcgtgc
    13801 aggccgaagg gcggacggcg attgaccacc cgcacatagg tgccgtccgc ctgcaattgc
    13861 caggccagct ggttgtcgcg caaggcatag gtgaaggcct ccgcgatgac ccgcttcttg
    13921 agcgccctgt cgtacacggg aaacgccacc tcgacccggc ggaagaagtt gcggtccatc
    13981 cagtcggccg acgagagata gacggtttcc tcgccgtcgg catggaagta gaacacccgg
    14041 gagtgctcca ggaagcgccc caccaccgaa cggacccgga tgttctcgga caggcccttc
    14101 acccccgcgc gcagggcgca cacgccgcgc acgatcagat cgatcttcac gcccgcctgg
    14161 ctggccaggt agagctcctc gatgacctgc gcttcgagca gggaattcat cttggccatg
    14221 atgcgggcct tgcggccggc ccgggccgcc tcggcctcgg cgcggatcag cgccaccatg
    14281 cccgcgtgca gggtgaacgg cgactgcagc agcgacttga gctcgcggcg cgcgcccagg
    14341 ccggtgagct ggccgaacac cttgtccatg tcctcgcaca ggcgcggatc ggccgtcagc
    14401 aggccgaagt cggtgtacag gcgcgccgtg cggggatggt agttgccggt gcccaggtgc
    14461 gcgtagcggc gcagccggcc gtcttcgcgg cgcagcacca gggccatctt ggcatgggtc
    14521 ttgtgcgcca ccacgccgta gaccacgtgc gcgcccacct cttccaggcg cgccgcccag
    14581 ttcatattgg tctgctcgtc gaagcgcgcc atgagctcga ccaccaccgt cacctctttg
    14641 ccggcgcggg ccgcggccag caggatctgc atcagctcgg aatcctcgcc ggtgcggtag
    14701 atggtctgct tgatggccat gacgtcggga tcgatcgcgg ccgccgtcag gaaatcgatg
    14761 accggctgga aggactggta ggggtggtgc aaaagacgat cgcgctcggc caccgccgcg
    14821 aacaattccg ccggcttgcc gcggagcttg tcgaacggcg cgggcaccgg cgcgagatag
    14881 agcgagaaca gcaggtcggg gcgcgtattg gtattgcaca gctgcatcag gcgcgacagg
    14941 ttcaccgggc ctggcacgcg gtaggtgtcg gccggcgtca gcgagaactc gcgctgcagg
    15001 tactgctcga gctcgggcgg catgatccag tcgatctcga gccggacggc cgaaccgaaa
    15061 ttgcgctgcg acagttcgcc ctgcaaagcg agccgcaggt tggtgacctc ttcctcgtcg
    15121 acgaacaggt cgctgttgcg cgtgacgcgc cactggtagc agccgtgcat cgtcaggccg
    15181 gggaacagct cgcccacgaa ggcgcgcagg agcgaggtca gcaggatata gccgtccggg
    15241 tggcccgaga gctcggccgg catgcggatg agccgcggca gcgcgcgcgg cgcctgcacc
    15301 accgcaatgg aagcctgccg cccgaaggca tcggccccgg acagcgagac gatgaagttc
    15361 aggctcttgt tgtagacgcg cggaaacgga tgcgccggat cgagcccgat gggcgtgagc
    15421 agcggcatga cttcgcgggt gaacacctcg tgcgcccatt gctgctgcgc ctcgtcccat
    15481 tcggaggcat ggtgcaggac gacgccctcg gcctgcaggc tgggcaggat gtgctcgttg
    15541 agcacccggt actggtgctc gaccagggag tggaccttgg cctgcacccg gtcgaaggcc
    15601 tcgtcgggcg tcatgccgtc cggggcgacg aggcccggcg tctggcgctg ctgctccttg
    15661 aggctggaga tgcggatctc gaagaattcg tccaggttgg cgctgacgat gcagacgtag
    15721 cgcagccgct ccaggatggg cacggacgga tgctcggcca tggccaggac ccgttcgttg
    15781 aacttgagca gcgacagttc gcggttgaac agccggggct cgccgccggc ccgcgccgcc
    15841 gcggccgatt cctggatggt tttttcgagt tctcgttgtt tcattgttgc catgtttatg
    15901 acacggtttg gtgatggaat gccggcaaaa taaggatttt ccaggctgag gcccgtcccg
    15961 cgggacgaaa tatcctcaca agtcgtgagg atatacaagc gcaagtcaac ccgccagtca
    16021 ggaccggctc tctataatcc cgactcttag atacttaccc gtatacgccg catggaccat
    16081 ctactggccg ccgtggacct agggtccaat agtttccgcc tctcgatcgg gcgcgtcgtt
    16141 caacagaacg gcatggcgca gatctaccag atcgaccggc tcaaggaaac cgtgcggctg
    16201 gccgccggcc tgggcacgga caagcagctc gatggttccg ccattgaacg cgccatcgcc
    16261 gtgctggagc gcttcggcga acgcttgcgc agtttccacc ccaaccgggt gcgggccgtg
    16321 gccaccaata ccttccgcgt ggcgcgcaac acgacggaat tcctgcccga cgccgaggcg
    16381 gccctgggct ttcccatcga cgtgatcgcc ggccgcgaag aagcccgcct gatctatctg
    16441 ggcgtgatgc acagcctgcc gccctccccc gaccgccgcc tggtgatcga catcggcggc
    16501 ggctccaccg aggtcatcat cggcaagggc ttcgagcccg gcctgatgtc ctcgctctac
    16561 atgggctgcg tgagctacag ccggcaattc ttcggcgacg ggctggtcga cgcgcaccgc
    16621 atgaagcagg ccgagatggc cgcgcgccgc gaggtcgagg tgatctccaa gcagtaccgc
    16681 aagatgggct ggaacgcggc cttcggctcg tcgggcacgg ccaaggccct ctacgccatt
    16741 ctcaccgaat gcggcttctc cacggccggc atcacccgcg ccgggatgaa caagctcaag
    16801 gagcgcatca tccgttcggg ccgcgtcatc cccgaggagc tgcccggcat caagctggac
    16861 cgggccgacg tgctgccggg cggcctggcc atcatgagcg ccatcttcga cgagctgaac
    16921 atcgacgcca tgcagacggg cgacggcgcc ctgcgcctgg gcgtgctcta cgacctgctg
    16981 ggccgcgacg acgcgcacga caagcgcgac gagtcggtgc gccagttcat gaagcgctat
    17041 cacgtcgacg cgcagcaggc ccgccgcgta cgcgaaacgg cgctggcgtt cttcgacagc
    17101 atcctccccg aagaccagga agaacacgcc gacctgcgcc gcgcgctggg ctgggcggcc
    17161 gatctgcacg aggtgggcct gtcgattgcc cacaacggct accaccgcca ttcggcctat
    17221 gtgctgcaga acgccgacat gccgggcttc tcgcaggccg accagcaatc gctcgccctg
    17281 ctgacgctgg cccaccaggg caagctgggc aaggtcgagc cgctggtacg cagccgcgcc
    17341 cagtggctgg ccatcctgag cctgcgcctg gccgtcttgc tgtttcgccg gcgccaggac
    17401 atatcgccct tgccgctcaa ggtgtcggtc aagggcaatt cgatcgtggt gaaagtggat
    17461 gcggggtggc tggaaagtca cccgttgagc gacttcaccc tgcgcgccga agactcagaa
    17521 tggcgcaagg tcggtttcgc gttcgagctg atcgagcgct gagatcaggg gccggctcat
    17581 gcgatgcatc agccggcttc cgacgcgacg cagcagcccg cccatgtcag cttccgcacg
    17641 gctgcccgaa ggaagctgaa gcccccccgg ggggcagcga acgtagtgag cgtgggggct
    17701 tcattcaacg ttctatgcgt tccagaccga agtggcggcg atagcggtcg tccatccgat
    17761 cggtcgtcag cagcacgggg aagtcggctg cgttgaaatc gggatcccag gccggctcgc
    17821 cgcacacctt ggcgcccagc ttcagatagc ccttgatgag cggcggcacg cgcgccggca
    17881 gcgtgctgtt caggcgctcg accggatagc ggtgcagcgg cttgacgcgc ggcgcgtccg
    17941 gatcctgcag gtgcttggac accgtgcgcc agacttcggc ggcggtcacg ccatcgtcgc
    18001 gcaggctcac gctggcgcag cccagcaggt agtgatagcc gccacggcgc aggaattcgg
    18061 ccaggccgga ccacagcagc atgatgaccg tgccgttgcg gtagttggcg tgggtgcagg
    18121 agcggcccac ctcgaccaat tggtcgcgca ggtcgttcag gccggaaaga tcgaattccg
    18181 actcggagta gtaaccgccc gcctcgcgcg ccttctgggg cgtgaggatg cgatacgtgc
    18241 cgaccacccg gttggtgtcg agttcgcgca ccatgaggtg ttcgcaccat gggtcgaagc
    18301 ggtcctggtc gataccgtct tccgcgtcgg ggaaaaccgc gcccatgtcc tcggtgaaga
    18361 cggtatagcg caaacgttgg atttgttcga cttcttcctg gctgcgggcg aggcctacgg
    18421 ccaggccgcg cgagtgcgta cccggccaag cctgggcgcc ttcggaaggg gcatcgatac
    18481 gtgcgagttc gagcattacg cgggactcct gtcagtgccg aaagtttgca cacacggcat
    18541 gtcagttatt tgactgattt attacatgat tgtgaattct acgcctacga ggaatgggct
    18601 ccccgaggaa atagtttgat acgcagtcag ccgcgcgtag gggaaaaaag atcgtcaagc
    18661 cgccagggag gccgccagct tgtgcgcgct ggcctcggcc agccgctcgt cctcggcctc
    18721 gaccatgagc cgcagcttgg gttcggtgcc cgaggcgcgg atcagcacgc gccccttgcc
    18781 ggccagatcg gcctcgaccg tgctgcgggc cgccgccagc ccggcgtgcg actgccagtc
    18841 ctggccgggc ttgagcggca cgttgatcat gacctgcggg aacatgcgca ggtcgcccac
    18901 ccattcggcc agcggcatgc cgccgcggcg cagagccgtc agcacctgca gcgcggcgat
    18961 ggtgccgtcg cccgtggtgt ggcggtccag gcatatcagg tggcccgagt tttcgccgcc
    19021 atagagccag ccgcgctcga gcagcatttc gagcacatag cggtcgccca ccttggcgcg
    19081 ttcgaaggga atgcccagct ccttgagacg cagctccagg ccgtagttgg tcatcagcgt
    19141 gcccaccacc ccgcccaccg ggcccgcggc catgcgcgag cgcacgatgg catagagcag
    19201 ttcgtcgccg tcgtacacgc ggcccgtggc gtcgaccatc tgcaggcggt cggcgtcgcc
    19261 gtcgagcgcg atgcccaagt cggcccggta cgccttgacc tcggccacca gcgcctcggg
    19321 atgcagcgcc cccacgccct tgttgatgtt gaggccgtcg ggagaagcgc cgatgacgcg
    19381 cacctcggcg cccagctcgc gaaacacgtg gggcgcggtg ttgtaggccg cgccatgggc
    19441 cgcgtcgatc accaggcgca tgccgctcag atcgaggtcg ttgggaaagg tgctcttgca
    19501 gaattcgatg tagcggccct gggcgtcgtc caggcggcgg gcgcggccca gttcttcgga
    19561 cgtcacgcag cccagcgggt tgccgagttc ggcctcgatc tgcagctcga tctcgtcggg
    19621 cagtttcatg ccctcggccg agaagaattt gatgccgttg tcctgatagg ggttgtgcga
    19681 ggcgctgatg acgatgcccg ccgtcagccg cagcgcgcgg gtgagatacg ccacggccgg
    19741 cgtggggatg gggcccgcca gcagggtgtc cacgccggcg gcggccaggc ccgcctcgag
    19801 cgcggattcg agcatgtagc cggagatgcg cgtgtccttg ccgatgagca gctgcgggcg
    19861 gcccggacgc ttgccgtgcg cgcgcgacag caccttgccg gcggcatagc ccaggtgcag
    19921 cgcgaagccg gcattgatca ccggaccgcc gacctcgccg cgcacgccgt cagtgccgaa
    19981 atacttgcgt tgttgactca tgcggagaat cccttttcca ccgactgcca gaccttgagc
    20041 gcatcgaccg tagccgccac gtcgtg
//
LOCUS       DASWPO010000269         7690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_956410, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000269 DASWPO010000000
VERSION     DASWPO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956410"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VGC69_14175"
     CDS             complement(<1..673)
                     /locus_tag="VGC69_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0296473.1"
                     /translation="MAITDVVVPQLSESISEATLLNWKKKAGEAVAADEILIEVETDK
                     VVLEVPAPASGVLAEIVEGDGATVTSGQLLAKIDTAATAAAAPAAAPAPVAAAPAPVA
                     AAPAPAAAPASTAAAGVASPAAAKILAEKGVDAAAVAGSGRDGRVTKGDALSATPAAP
                     AGAAKPAAAAPAAPPTLSLDGRPEQRVPMSRLRARIAERLLQSQQENAILTTFNEVNM
                     KAVIDL"
     gene            complement(758..3628)
                     /locus_tag="VGC69_14180"
                     /pseudo
     CDS             complement(758..3628)
                     /locus_tag="VGC69_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813631.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
     gene            4110..5330
                     /locus_tag="VGC69_14185"
                     /pseudo
     CDS             4110..5330
                     /locus_tag="VGC69_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            5414..5869
                     /locus_tag="VGC69_14190"
     CDS             5414..5869
                     /locus_tag="VGC69_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEY0296474.1"
                     /translation="MLQDALLAWLHYLAIFLLAGLLCIEAALLRPGLAAQTALRLARY
                     DLAYGLSALAVLATGLLRMAYGAKGAAFYLGNPWFHAKIGLFVLVGLCSVIPTLRLLR
                     WRRQARNEATFAPSAREIARVRRWVMIEAHLLILLPLCAVMMARGIGLS"
     gene            complement(5968..7266)
                     /locus_tag="VGC69_14195"
     CDS             complement(5968..7266)
                     /locus_tag="VGC69_14195"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0296475.1"
                     /translation="MNLSDKKATLSFSDGSPAIELPVYKGTIGPDVIDIRKLYGQSGM
                     FTYDPGFLSTAATQSGITYIDGDKGELLYRGYPIEQLATQCDYLDTCYLILNGELPTP
                     EQKKEFSELVSQHAMVNEQMHVFLRGFHHDAHPMAMLTALVGALSAFYHDETDITKAE
                     DRYLAAVRLIAKLPTLVAMVYKHSRGEPFVYPKSGLSYAGNFMHMMFASPSGDYKVNE
                     VVERALDRIFILHADHEQNASTSTVRLCGSSGTDPIAAIAAGVACLWGPAHGGANEAC
                     LNMLEEIQASGGLATVGKFMEQVKDKNSGVRLMGFGHRVYKNYDPRAKLMQETCKEVL
                     GALGLENDPLLKLAMALEKIALEDDYFVQRKLYPNVDFYSGIVQRAIGIPTPLFTGIF
                     TLARTSGWIAQWAEMIADPEYKIGRPRQLYTGEAARQIKR"
     gene            complement(7288..7548)
                     /locus_tag="VGC69_14200"
     CDS             complement(7288..7548)
                     /locus_tag="VGC69_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY0296476.1"
                     /translation="MSSLTELERARLRWRARRGLLENDLIITRYLDAYEHQLTDEDVS
                     ALTQLFETGDNELLDILLARTELHGALDTPRLRNIVGLMRAL"
     gene            complement(7601..>7690)
                     /gene="sdhB"
                     /locus_tag="VGC69_14205"
     CDS             complement(7601..>7690)
                     /gene="sdhB"
                     /locus_tag="VGC69_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248745.1"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol; the
                     catalytic subunits are similar to fumarate reductase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY0296477.1"
                     /translation="MNCVDVCPKGLNPTKAIGKIKELMVRRTV"
BASE COUNT         1231 a         2427 c         2577 g         1455 t
ORIGIN      
        1 gcaggtcgat gacggccttc atgttgactt cgttgaaggt cgtcaggatg gcgttttctt
       61 gctgcgactg cagcaggcgc tcggcgatgc gggcgcgcag gcggctcatg ggcacgcgct
      121 gttcggggcg gccgtccagc gacagggtcg ggggcgcggc cggcgcggcg gcggcgggct
      181 tggcagcccc agcgggcgcg gccggggtgg cagacagggc atcgcccttg gtcacgcggc
      241 cgtcgcggcc cgaaccggcc acggcagcgg cgtccacgcc cttctcggcc aggatcttcg
      301 cggcagcggg cgaggccacg ccggcggcgg cagtgctggc cggggcagcg gcgggcgcgg
      361 gcgccgcggc gacaggcgcc ggggcagcgg caaccggagc cggcgcagca gcgggcgcgg
      421 cggcagccgt ggcggcggtg tcgatcttgg ccagcagctg gcccgaggtc acggtcgcac
      481 cgtcgccttc gacgatttcg gccagcacgc ccgaggcggg cgccggcact tcgagcacga
      541 ccttgtcggt ctcgacctcg atcaggattt cgtcggcggc gacggcttca cccgccttct
      601 tcttccagtt gagcagcgtg gcttccgaga tggactcgga cagctgggga acaacgacgt
      661 cggtaatagc catgatgatg gtttccgttt atttgatttg atgttctgca tggccccggg
      721 cggcatgcgc atggcatgcc gcccggggac ctgtcgatta tttggtcagc atgaagccct
      781 tgaacctggg cgcgtaggcc tgctccagca gggccttgag ctgttcctgg tgcttggcca
      841 ggtagcccac ggccggcgaa gccgaggccg gacggccggc gtagcccagc ttctggcctt
      901 cggccatgtt ctcatacagg tgatgctgca cgtagaacca ggggccctgg ttctgcggct
      961 cgtcctgcac ccagatcacc tcggtggcct tgccgtactt cttcagttcg gtctcgaaga
     1021 tcttgtgcgc gaagggatag agctgttcga cgcggacgat ggccacgtgc tcggcgccgc
     1081 gctctttacg ggcgtggacc aggtcgtaat agatcttgcc cgagcaagcc agcacgcgct
     1141 tgaccgacgc gggcttgacg gcctcgtcga cttcgccgat caccggatgg aagtggccct
     1201 tggccaggtc cgacaggggc gagccggcgt ccttgttgcg cagcagcgac ttgggcgtga
     1261 agatgaccag cggcttgcgg aaggggcgga tcatctggcg gcgcagcagg tggaagatct
     1321 gcgcggccga agtcggctgg acggcctgga tgttgttgtc ggcgcacagc tgcaggaagc
     1381 gctcgatgcg ggccgacgag tgctcgggac cctggccttc gtagccgtgc ggcagcatca
     1441 tggtcaggcc gcactggcgg ccccacttgg cttcgccggc gctgatgaac tggtcgatca
     1501 cgacctgggc gccgttgacg aagtcgccga actgggcttc ccagatggtc agcgtgttgg
     1561 gctcggagca ggcgtagccg tattcgaagc ccagcaccgc ctcttcggac agcacggagt
     1621 cgatcacggt gaagggcgcc tggccttcgg agacgttctg cagcgggatg taggtgccgt
     1681 cgttccagcg ttcgcggttc tggtcgtgca gcacggcgtg gcggtgcgtg aacgtgccgc
     1741 ggccggcgtc ctggccggtg atgcggatgg cgtagcccga ggccaccagc gtggcgaagg
     1801 ccatgtgctc gcccatgccc cagtcgaggt tctgctcgcc acgggccatg ttggcgcggt
     1861 cgcccagcag cttcttgacc agcgggtgca ccgtgaaacc ttccggcacg gtggtgatgc
     1921 gctcgccgat gcgcttgagc tcggccagcg gcacggccgt gtcggccgtg tcggtccact
     1981 tggcgttcag gaagggcgac cagtcgatgg cgtacttgct cttgtagtcg gtcagcacgg
     2041 gctcgaccgt cttgtggccg tcttccatca gctggcggta gtccttgacc atctggtcgc
     2101 cttcgccgtc ggccagcacg ccttgcgcgg tcagcttgtc ggcgtacacc ttgcgggtgc
     2161 cgggatggtg gccgatggtc ttgtacatca ggggctgcgt gagcgcgggg gtgtcctgct
     2221 cgttgtggcc cagcttgcgg aagcagacga tgtcgaccac gacgtcgtgg tggaactcca
     2281 tgcgatagtc cagcgccagc ttggtgacga acaccacggc ttcggggtcg tcgccgttga
     2341 cgtggaacac cggggcttcg atcatcttga ccacgtcggt gcaatacagc gtggagcgcg
     2401 cgtcgcgcgg gtcggacgtg gtgaagccga tctggttgtt gatgacgatg tgcagcgtgc
     2461 cgcccgtgcc gtagccgcgc gtctgggcca ggttgagcgt ttccatcacg acgccctggc
     2521 cggcgaaggc cgcgtcgccg tgcaccagca ccggcaggat ctgcatgcct tcgtggtcgc
     2581 cgcggcgctc catgcgggcg cgggcgctgc cctcgaccac cgggttgacg atttccaggt
     2641 gggaggggtt gaaggccagc gacaggtgca ccgggccgcc gcggcccgag atgtcgctgg
     2701 agaagccgtt gtggtacttc acgtcgccgt cggtcaggcc ttcggcgtgc ttgccttcga
     2761 actaggcgaa caggtcgccg ggcatcttgc ccatgatgtt cacgagcagg ttcaggcggc
     2821 cgcggtgggc catgccgacc acgatttcct gaacgccgac atccgtgccg tggttgacca
     2881 cttcgtccat cgaggcgatg aagctctcgc cgccttcgag cgaaaagcgc ttctggccga
     2941 cgtacttggt gtgcaggaag cgctcgaggc cttcggcgtc ggtgagctgc tgcaggatgt
     3001 ggcgcttttg ctcgttggtg aaggtgggcg tggcgcgcgt ggtttcgagg cgctcgcgga
     3061 tccagcgctt ggcggcggga tcggacacgt gcatgaactc ggcgccgatg gtgcggcagt
     3121 aggtgtcgcg cagcgccttg atgatctcgc gcagcgtcat ggtgctggcg gtcgtgaagt
     3181 agctgttggt cgcggcgtac tgctggtcga gatcggcttc ggtcaggccg tagaaggccg
     3241 gatcgagttc gggaatgttg ggacgttcct ggcgcttgag cggatcgagg tcggcccagc
     3301 gcgagcccag gctgcggtag gcgccgatca gcgactgtac cgacacctgc ttgctggcca
     3361 ccgacatatc gggctcggcg gtgtgcagcg tgaaggcgtt ggcgcgggcg cgttcggcga
     3421 acgactggac gatgggcgcg tgggcctggt cgcgggtggt tgcctggccg tcggtggcgg
     3481 gagcgtgctg gagctggtcg aaatacgacc gccagttgtc tggcaccgaa ccaggattat
     3541 cgaggtacga ttcgtagagc tcttcgacat aaggggcgtt gcccccgaag agataggagt
     3601 tggatagaga ttcttgttcc gaagacatat ttgcttcacc ttgccgggtg cctcacccga
     3661 tacgatgcag gaaaaccttc cgcgacacgg gcttcccggt tagcggatag caggagcaga
     3721 aggcgccgta ctaaaattca aaacctgtac aaaaagagcc tttaaagcta ccgcttggag
     3781 tatacccctt tgccaagcgg ctcttttact ttttatggac tgttttgtag caccgggaca
     3841 tgacagtcaa gcgaattatc tcgccgcgtt ttcgatgcca tcccggcgaa ttgcccaatc
     3901 tgaattttcc ctgtctcggc ctatccgcgc gggccgtttt gccttgccga accgcgtcgc
     3961 gcgcccagga cgtcatgttt cgttacaccg cgccaggccc gcggccgcac acggtcaagc
     4021 gcgcaacccg gctttctgtt gaaatttcac ttttcccgct tttcccttct ccgcttttcc
     4081 ctactcatca aagaatacgc agccccctca tggaccaaac tctcatcaag caggcgctgg
     4141 actaccacca gtaccccacc cccggcaaga tctcggtcaa gcccaccaag ccgctcacgg
     4201 gcagggacga cctgtccctg gcctattccc ccggggtcgc cgccgcctgc atggccatcc
     4261 atgccgaggg cgtcgacgcc gcctcgcgct acacctcgcg cggcaacctg gtgggcgtga
     4321 tcaccaacgg cacggccgtg ctgggcctgg gcaatatcgg ccccctggcg gccaagcccg
     4381 tcatggaagg caaaggctgc ctgttccaga agttcgccgg catcgacgtc ttcgacatcg
     4441 aactggccga gaccgacccc gacaagctgg tcgacatcat cgccgcgctc gaacccaccc
     4501 tgggcggcgt caacctcgag gacatcaagg cccccgagtg cttctacatc gagaagcagc
     4561 tgcgcgagcg catgaagatc cccgtcttcc acgacgacca gcacggcacc gccatcatct
     4621 cctcggccgc ggtgttgaac ggcctgaagg tcgtgggcaa ggaaatcggc caggtcaaga
     4681 tagccgtatc gggcgccggc gccgccgcca tcgcctgcct ggacctgatg gtcgacctgg
     4741 gcatcaagcg cgagcacatc ttcgtggtcg attcgcgcgg cgtcatctgg gaaggccgcg
     4801 acgccaatat ggagcccaac aagaaacgct acgcgcagca gaccgacgcc cgcacgctcg
     4861 ccgacgtggt ccgcggcgcc gacattttcc tgggttgctc cgccgctggc gtgctgacgg
     4921 cggagatggt caagaccatg gccgacaagc cgatcatcct ggccctggcc aaccccgagc
     4981 cggaaatccg ccccgaagtc gcccgcgcag cccgccccga ctgcatcatc gccaccggcc
     5041 ggtccgacta tccgaaccag gtcaacaatg tgctgtgctt ccccttcatc ttccgcggcg
     5101 ccatggacgt gggcgccacg cgcatcaccg aagcgatgaa gctggccagc gtgaaggcca
     5161 tcgccgccct ggcctgcagc gaagccgagt tcggtcccga gtacatcatc cccgacccct
     5221 tcgacccgcg cctgatctcc gaagtcgcgc cggccgtggc gcaggccgcc gtcgaatcgg
     5281 gcgtggccca gcgccccatc gccgacatcg aggcctaccg cgagaagctc aaggcctggc
     5341 gcgcaagcgt ctaggcagtc agccggcgcg gggcgtcctt cccgacctgg caacgccctt
     5401 tcaaggagtt cccatgttgc aagacgccct gctcgcctgg ctccactacc tggccatttt
     5461 cctgctggcc ggcctgctgt gcatcgaggc cgcgctgctg cgccccggcc tcgccgccca
     5521 gaccgccctg cgcctggcgc gctacgacct cgcctatggg ctgtccgcgc tggccgtgct
     5581 ggccacgggc ctgctgcgca tggcctatgg cgccaagggc gcggcgttct acctgggcaa
     5641 cccctggttc cacgccaaga tcggcctgtt cgtgctcgtc gggctctgct ccgtcatccc
     5701 caccctgcgc ctgctgcgct ggcgccgcca ggcccggaac gaggcgacct tcgcgccctc
     5761 ggccagggaa atcgcccggg tgcgccgctg ggtcatgatc gaggcgcatc tgctcatcct
     5821 gctgccgctg tgcgccgtca tgatggcgcg gggcatcggg ctgtcgtagt tccaatacgg
     5881 ccgcgcaaaa cgcaaaaacc cctggcctgg gccaggggtt tttcattgcg ggccgggtga
     5941 acggccccgc gccgcaaggc ttgccggtca gcgcttgatc tgacgcgcgg cttcgccggt
     6001 gtacagctgg cgcggacggc cgatcttgta ctcggggtcg gcgatcattt cggcccactg
     6061 ggcgatccag ccgctggtgc gggccagggt gaagatgccg gtgaacagcg gagtcggaat
     6121 gccgatggcg cgctgcacga tgcccgagta gaagtcgacg ttcgggtaga gcttgcgctg
     6181 cacgaagtag tcgtcttcca gggcgatttt ttccagggcc atggccagct tgagcagcgg
     6241 gtcgttttcc aggcccaggg cgcccagcac ttccttgcaa gtttcctgca tcagcttggc
     6301 gcgcgggtcg tagttcttgt agacgcggtg gccgaagccc atcaggcgca cgcccgagtt
     6361 cttgtccttg acctgctcca tgaacttgcc cacggtggcc aggccgccgc tggcctggat
     6421 ttcttcgagc atgttcaggc aggcttcgtt ggcgccgccg tgggcagggc cccacaggca
     6481 agccacgccg gcggcaatgg cggcgatggg gtcggtgccc gacgagccgc acaggcgcac
     6541 ggtcgaggtc gaggcgttct gctcgtgatc ggcgtggagg atgaagatgc ggtcgagcgc
     6601 gcgctcgacg acctcgttca ccttgtagtc gccgctcggg ctggcgaaca tcatgtgcat
     6661 gaagttgccc gcgtacgaca ggccgctctt gggatagacg aagggctcgc cgcgcgagtg
     6721 cttgtacacc atggcgacca gcgtgggcag cttggcgatc aggcgcacgg ccgcgaggta
     6781 gcggtcctcg gccttggtga tgtcggtttc atcatggtag aaagccgaca gcgcgccgac
     6841 cagggcggtc agcatggcca tggggtgggc gtcgtgatga aagccgcgca ggaagacatg
     6901 catctgctcg ttcaccatcg cgtgctggct gaccagctcg ctgaattctt ttttctgctc
     6961 gggcgtgggc agttcgccgt tcaggatgag gtagcaggtg tcgaggtagt cgcactgggt
     7021 ggccaattgc tcgatggggt agccgcggta cagcagctcg cccttgtcgc catcgatgta
     7081 ggtgatgccc gactgcgtgg cagccgtcga caggaaaccc gggtcgtacg tgaacatgcc
     7141 cgactgaccg tacaacttgc gaatgtcgat cacatcggga ccgatggtgc ccttgtaaac
     7201 cggaagttcg atggcggggc taccgtcgga gaaggataga gtggcttttt tgtcggacaa
     7261 attcatggtt gtttcctcgt agatatatta gagcgcccgc atcaaaccga cgatattgcg
     7321 cagccgggga gtgtcgagcg caccatggag ttcagtacgc gccagcaaga tgtcgagcaa
     7381 ttcgttgtct ccagtctcga acaactgcgt taacgcgctt acatcctcat cggtaagctg
     7441 atgctcgtaa gcatccaggt accgagtaat gataaggtca ttctcgagca ggcctcggcg
     7501 cgcacgccag cgcaggcgcg cacgttccaa ttctgtcaga ctactcatga aaaacctctg
     7561 aagccaagat aaagccccga ctctcgtcag ggctttttca ttacacagta cggcgaacca
     7621 tcagttcctt gatcttcccg atggccttgg tggggttcag gcccttggga cagacatcga
     7681 cgcagttcat
//
LOCUS       DASWPO010000270        16366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_242370, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000270 DASWPO010000000
VERSION     DASWPO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 16366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16366
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242370"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGC69_14210"
     CDS             complement(<1..327)
                     /locus_tag="VGC69_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296478.1"
                     /translation="MHQDHALPDARRPLRIAAEEGPIELSFDALQAYHGHGALAMLAL
                     IYQGQRGALACLETAGQPVPRTELSVVSGHPGPGVRDAFEFVTRAVTRGCYVVDLTLP
                     YARYGNP"
     gene            complement(382..2517)
                     /locus_tag="VGC69_14215"
     CDS             complement(382..2517)
                     /locus_tag="VGC69_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0296479.1"
                     /translation="MQARYLFATLVSANLLAAATVRAQETAAQLPAISVIGDTADDGR
                     IAPKGSDSAPAQIRHTATQPVTVVDHQEIEMVNPLSTLDLFDQVPGATVNRTGGIGGT
                     VFLRGLNTNDMRVPVFIDGDRFRGRNTLQFMLISPTEIEQMEVVRGPASSLYGSDGLG
                     GLINFVTKRAHGDLDHDFQVTGGEASVTYRNNGNGVQSDVAMEASGSGFDLRAYVTGR
                     RSGNYDTPAGTVPNSDYRSGGGGVVLGYMPDAKQRFEVSARVTTVDAGIAGSVPPYPT
                     SIGRYAPLQVAEGRLAYAGEFDHSVVSKLDASLYIDDFNTHISTVNQANPARITDTIS
                     HVIGPVAIGGHVAATVPWGPTENVFGMDFMNEIRPGAQSRSEVTTPSGTTRTGYAPSG
                     PNTYQTNIGAFANTTWKATRDLTVTAGGRFDWFHSDVDLSALPSPNLLPAFQAAQNAY
                     DTATTGSLGLSYRATDVLELLGSVGTSFRMPWTSELFTSGYTGTSYTVPNPSLKPERG
                     TTLEAGPRLHFDNATVGLTAFNSDYRNFIQTVNTTYMGLPATERENVGKARIQGVEAD
                     WRWQVTPRINVYGNASYLRGTDRTANAPLPSIAPLSGLAGVQYVSPGQSYSLSGELQW
                     AGHQSRHASSEYPSTGYAVVNLYAQLQLDRLGLPQLGNTQLIFGVTNLFNADYRTAAT
                     TSNVAYPMTYLNPLIEPGRTVSLTLRTRF"
     gene            complement(2545..3180)
                     /locus_tag="VGC69_14220"
     CDS             complement(2545..3180)
                     /locus_tag="VGC69_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296480.1"
                     /translation="MSAAEPVIAALDAGTYYHHRTFHTASLRPCIDRTIYVRELDDAA
                     LRGCDALIVSCRTNPDLLAPHRERFARFLAAGKTLVAMGETGAERWLDGVAWTPCEVN
                     FWWWKEPGADSGLRLAAADHPLFRYIGLADATWHQHGTFQPPAGAVSLIDKAGAGSVL
                     YEHDRGGAGRLIVTSLDPMYHHGSYFMPATTRFLHGFLRWLKDSTPAAGRP"
     gene            complement(3177..3779)
                     /locus_tag="VGC69_14225"
     CDS             complement(3177..3779)
                     /locus_tag="VGC69_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296481.1"
                     /translation="MPSATAYLNHWPGRGPAALTGGPNAPAEPLALYDLAHADLRRWR
                     SLLVPAHADQRFLAGQRERLEVFLLSGGTLVFNGHVAYPFLRWLRPFQPVAHRGLDGL
                     RVHRAEDHPIFEGVDPEHLTFRRGVAGFYARGGNPPAPGARVLHTLGPDALPVDWLLE
                     LPGGGRLLVHSGNDLWMYAGGGDSAARVVPQLLAWLEART"
     gene            complement(3785..4522)
                     /locus_tag="VGC69_14230"
     CDS             complement(3785..4522)
                     /locus_tag="VGC69_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296482.1"
                     /translation="MIEVDDLAVRAGGRILLEGLSLRVRAGEILAVLGPNGRGKTTFL
                     RTVLGLQAAAAGTVRLGGHAAYVPQQTDTLFSYDTLDMVSMGRARHLRWYASPGPGDL
                     HAARACLRAVELEHLSAQSFQTLSGGQRQLVCIARAMASESPIMLLDEPTAALDLRNQ
                     DIVLAVLRRLAKERGLAVVFSTHHPQHAQHIADQTLLMHDEGCEAGPTGLMCTDERLS
                     RLYRLPVHVAALRHDGRDVRGVIPLFR"
     gene            complement(4515..5588)
                     /locus_tag="VGC69_14235"
     CDS             complement(4515..5588)
                     /locus_tag="VGC69_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY0296483.1"
                     /translation="MNAFIERRGVFAGVPPALSWRWLLMGAALPASMLVALCIGRYPL
                     PLSHVAAMLLDACLPAGGHGWLAAGGDVERRILFQVRLPRVLLGALVGAGLAMCGSTL
                     QGMFRNPLVGPQILGISSGASFGGCLSILLFSSLTATLGMSFAGGLVAVAIVYMLGRV
                     QRRTTMLMLLLAGVVSSGFFSALTSMVTYFADPNDTLPAMVFWLMGSFATASYAKLAA
                     AAVPALAGMGLFYALRFRINVLSLGDEQAAALGLAVEPLRWVLLACTALVTSASVAVS
                     GTVGWVGLVVPYIARLFVGADQRRQLPAAALVGGAYMVLVDTAARSLTSAEIPLGVIT
                     ALLGAPLFVVLLRRARRKDPGHD"
     gene            complement(5572..6657)
                     /locus_tag="VGC69_14240"
     CDS             complement(5572..6657)
                     /locus_tag="VGC69_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296484.1"
                     /translation="MSPPLKSLPFGRAAALACLALLCRAAYAEPIAFTDMLGNKVTLA
                     GPTVHAVTVPMPASSLFMSLDGGPAHLAGMHPTAYALMRDGLLARIFPQAAGVRSDIT
                     RSGFTPNVETLLQMRPDLVWQWGHMGDDLLAPLRNAGLPVAALRYGTEADTREWIRLM
                     GMSLDRQAQARQQLQWRDQVHAAIARVTDSMGPAERPKVLYLARRFPQYRAAGRNTNF
                     EDDITLAGGRNVASSVTSSQPLDVEQILAWAPEVILLNNFERALTPQTIYQDPLLADV
                     PAVRNRRVYKVPEGGYLWDPPNQESPLYWQWLSVMLHPDKFDWPLRALIAQAYPRLYG
                     YTPSEQDIDAVLRTAMNDGSAGYERFH"
     gene            6750..7469
                     /locus_tag="VGC69_14245"
     CDS             6750..7469
                     /locus_tag="VGC69_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0296485.1"
                     /translation="MTFVNPIHTNAAHGLQALRAHPWFKELDERELTPLAAASRWLAY
                     APGREIFAEGHAIASCLLVVSGCVQGLRYTADGGEKIFGQVRPGGMVSALSVFMREPR
                     HLHSVRAVEAGEGYLLDAAAFRRLCEANALLACRVLRHSADLVRHHTDQIDWLTSSSA
                     EERLAEYLLRVGKPHAQEPVELPLTHSQIAIKLGMRAETLSRLLAKWRRQGWISDQRG
                     ALRVLDVAYLKRLAEAGKTPL"
     gene            complement(7527..8324)
                     /locus_tag="VGC69_14250"
     CDS             complement(7527..8324)
                     /locus_tag="VGC69_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEY0296486.1"
                     /translation="MTTIAQISDIHVRPQGVLYQGVVDSNAMFARAVETLNRLAPAPD
                     MVLITGDLVDQGAEDEYAMLQALLAGLRLPFAFLPGNHDRRDTLRAAFRDHPHIPSGE
                     AFDFAMDVGAIRLIALDTSVPDAHHGELSTAQLGWLDRELAACRGRPALVAMHHPPFA
                     TGIPYLDRYGLHDAQAFADVIRAHDHVDRVIAGHVHRAMHARLGGTPVLTCPSTATQI
                     ALRLEPDAKPASYLEPPAFMLHRWAGDAPAVSHLVHIGDYGTAFPFA"
     gene            complement(8381..9940)
                     /locus_tag="VGC69_14255"
     CDS             complement(8381..9940)
                     /locus_tag="VGC69_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0296487.1"
                     /translation="MHRPESLAHGMRLVYVAALLGLGGCASASLDLAPAGPDRPWHPQ
                     TNSDGEILAAPPALSGDAAGGGYTLPANKKLAQLPPATDVDSSRDYALPELIDLAQSA
                     NPRTRIAWNAARNAALAVGIAKSAYLPYLAATAMGGYAAGHGSGSNNLLGSASDDAST
                     HGTVSALTLQWLLFDFGGRDARVQAAQQTSIAANIALTAVHQQLIHEVSVAYYQYDAA
                     RARAANARQALANAGTLAAAARARYQHGAGTVVETAQAEQNLAQARFALTSALGVESD
                     AYLGLISALGISPLSKPRIAPLPRRALPSDLGASVETLVADALARRPDMLGAYAAQQA
                     EQAKVKAAETEFLPKVFLSASTSYSSGQSAITAIPAVGQQPAAVNLDNHRAGSAVLLG
                     VTIPIYDGGLRSAVLMQAHNDAASAQLRLARTRDEAVRQIVAGQNALRTSLASHEAAA
                     VLIKASQTTYDAALATYRNGVGSLTEVTLAQNQMLLARNAYADSYSAALSAIASLALA
                     TGSIGSGDGQP"
     gene            complement(9927..11789)
                     /locus_tag="VGC69_14260"
     CDS             complement(9927..11789)
                     /locus_tag="VGC69_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0296488.1"
                     /translation="MGPPADKARALAPRDLLALLAPFPGRMATSLRVALICALTVLVT
                     AAYGTPEASLSVYIVFFLIRPERVTGVLLPAIMLLFVTLVIGLVMLIAVFSIDYPVLR
                     LGCMAGVSAALLYLTSASKLRPVGAIFAMIIGFGLDELGLVPLGEAATRELLYAWLMA
                     AIPVGLAMLVQLLLAPSPRRLAGLRLAARLAASARRLRQPDTPRDDFAQCLREGNRQI
                     ESWLKLAGGATAADLAALRQATAASTTVMIAVDLLDTAPSARLPDAIAHPLADTLDAM
                     AGMLAQGGYPVDIQLDLPAVQGLPPLAQAALADLHDAIVHFTEAPPAAAQEAPQPARH
                     KAGFMLPDARSNPAHLAYALKTTAAAMFCYALYTQLDWQGIHTCFITCYIVSLGTTAE
                     TVQKLALRLAGCLVGALAGTAAMVFIVPSLSSIGELMLLVFTASWASAWVAVGSPRIS
                     YAGLQIAFAFFLCVIQGSSPGFDLTIARDRAIGILLGNIVVYLVFTRIWPVSIAGRID
                     QAIAALLQQWRRIAQLGDAAERRAQAADALMRHQAIGQDLGLIPYEPSWVRPGADWVA
                     ARRRALAELTALEAPLFLAADRMPGNATLQAQLRGIDAITADLMPKEPAVHAPA"
     gene            complement(11770..12819)
                     /gene="mdtN"
                     /locus_tag="VGC69_14265"
     CDS             complement(11770..12819)
                     /gene="mdtN"
                     /locus_tag="VGC69_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtN"
                     /protein_id="HEY0296489.1"
                     /translation="MKLAGIRKPSVKGRAIAALIVVLGLAALAFAYHRSTNYPSTDDA
                     TIDADYVHIASPVGGRIVRLAVRENQRVAKGDVLFEIDPVPYRLTVAQAQAEVEMARA
                     ALESRRKTLIAERANAAIAGEQRQRAEQNYALAARTTQRLAPLSAQGYIPAQQYDQAQ
                     VAQQDAAVSLRQAQAQHQTASSTIGDDADALAALHAREAALARARHALDDTVVYAPHD
                     GYVTGLSILAGETVAPSQSIFTLIDAGEWFAVANFRETELVRIAPGDCATVYSMIDRG
                     VALPGKIVGIGAGVLDTDRVNLPSSLPLVQKSVNWVRVAQRFPVRVQLEEADSKLLRV
                     GASAVVEVRHGPACR"
     gene            complement(12816..13028)
                     /locus_tag="VGC69_14270"
     CDS             complement(12816..13028)
                     /locus_tag="VGC69_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296490.1"
                     /translation="MGAPSYALFGAYFPHWLLSAVAGVAAALAGHRLFVMTGWSETVP
                     YPLSVCTAIGLVAGVLVWGIGTGQIP"
     gene            13316..14857
                     /locus_tag="VGC69_14275"
     CDS             13316..14857
                     /locus_tag="VGC69_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0296491.1"
                     /translation="MFANFSIRARVSLAFGLLVVLMLVVAALGELGALRGKNALHDTY
                     SVQLASAVALGDSKYNLAIARVAIDRALLGSNAADLPGLAQKARDYLATSQKAYARYL
                     ALPKRSEEAQLAKAVSSDWDKLLAGAITPALQALQNGDAATANRFTMEVMPPLSLNLT
                     KSLDRLNAWLMQQGQDNYGQFQQTLTRISLAAGVALAFGVMVALACTVGLHRAISRPL
                     GHALEVCSSMAQGNLTQRIEVRGRDEMSQMMLGLSQMQAGLRGTVSTMSESCESMATA
                     TQQIASGNSDLSRRTESQAAALQQTAASIEELTATVRQNSGSASSASGLANQATGVAQ
                     TGGEVMARVVATMSGIREQSTKIATIIGTIESISFQTNILALNAAVEAARAGEQGRGF
                     AVVAAEVRTLAQRSASAAREIKTLIDGAVGRIAEGSGLVDSAGRTMDEIVQSIQRVSG
                     IMDEVATASREQGDGIEQVNQAISQMDEATQQNAALVEETTAAAVSLAEQAQVLRDAA
                     ARFRI"
     gene            14993..15529
                     /locus_tag="VGC69_14280"
     CDS             14993..15529
                     /locus_tag="VGC69_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY0296492.1"
                     /translation="MRIYPGLKLVAALACGAVAALPMSAVAAETASGELLGSDGAAHG
                     TVQVTAAPAGVLLRIEAKGMAPGWHGVHFHEKGDCGAPKFTSAGAHVHSVTPVVHGLL
                     NANANDAGDLPNIFVGADGAMTVELYSTLVSLKPGGSRPALLDADGAALVIHANPDDY
                     QSQPIGGSGDRVACAVLH"
     gene            complement(15596..>16366)
                     /locus_tag="VGC69_14285"
     CDS             complement(15596..>16366)
                     /locus_tag="VGC69_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0296493.1"
                     /translation="GAYLVQGAGHCGSCHTPRSVAMNEIALDDSDPRYLSGALLDGWY
                     APSLRGDANTGLGRWSEADIAQFLKTGRNSHAVVFGSMADAFNNSTQFMSDEDLKAIA
                     HYLKSLPGTPGKDGPPWQPDTAPNDALALGQRLQIPGAQTFMARCSACHGAQGRGQGQ
                     WIPPLAGSAASMAKENASSINVTLNGSARIVANGVPDAYRMPSFRNQLSDQELADVLS
                     FVRTSWGNQGGAVKPADVQSLRERTNPASSNVIVLQMR"
BASE COUNT         2613 a         5361 c         5972 g         2420 t
ORIGIN      
        1 gggattgccg tagcgggcat acggcagcgt caggtcgacc acatagcagc cgcgcgtgac
       61 cgcgcgggtc acgaactcga aggcatcgcg cacgccgggc ccgggatggc cgctgaccac
      121 gctcagttcg gtgcgcggca cgggctggcc ggccgtttcc aggcaggcca gcgcgccgcg
      181 ctggccctgg tagataaggg ccagcatggc cagggcgcca tggccgtgat aggcctggag
      241 ggcatcgaag gaaagctcga tggggccttc ctcggcggcg atgcgcaggg gacggcgggc
      301 gtccggcagg gcgtggtctt gatgcatggt caggttcctg aaaaaaggcc gccccgccac
      361 ggggcggccg gaagtcccta tttagaaccg ggtgcgcagc gtgaggctga ccgtgcggcc
      421 gggttcgatc agggggttga gataggtcat gggataggcg acgttggatg tcgtcgcggc
      481 ggtgcgatag tccgcgttga acaggttggt gacgccgaat atgagctgtg tgttgccaag
      541 ctgcggcaat cccagccggt ccagctgcaa ctgcgcatag aggttgacca cggcatagcc
      601 ggtggaaggg tattcgctgc ttgcgtggcg ggactggtgc ccggcccact gcaattcgcc
      661 gctcagcgaa taggactggc cgggactgac gtactgcacg cccgccaggc cgctcagcgg
      721 cgcgatcgat ggcagcggcg cgttggcggt acggtccgtg ccgcgcaggt agctggcatt
      781 gccgtaaacg ttgatgcggg gcgtcacctg ccagcgccaa tccgcctcca cgccctggat
      841 gcgcgccttg ccgacgtttt cgcgctcggt cgccggcaag cccatgtagg tcgtattgac
      901 cgtctggatg aagttgcggt agtcgctgtt gaacgccgtc aggccgaccg tggcgttgtc
      961 gaaatgcagg cgcgggcccg cttccagcgt ggtgccgcgc tccggcttca gggaggggtt
     1021 cggcacggtg tagctggtgc cggtatagcc cgacgtgaag agttcggaag tccagggcat
     1081 ccggaacgac gtgcccacgc tgcccagcag ttcgagcacg tcggtggcgc ggtacgacaa
     1141 gcccaggctg cccgtggtgg cggtgtcgta ggcgttctgc gcggcctgga aagccggcag
     1201 caggttgggc gaaggcagcg cggaaagatc gacgtccgaa tggaaccagt cgaagcgccc
     1261 gcccgccgtc acggtcaggt cccgggtcgc tttccaggtc gtgttggcga atgccccgat
     1321 gttggtctgg taggtattcg ggccggatgg cgcatagccc gtgcgggtgg tgccggacgg
     1381 cgtcgtcacc tcggagcggg attgcgcgcc cggacggatc tcgttcatga agtccatgcc
     1441 gaagacattc tcggtcggcc cccaaggcac ggtggcggcc acgtgcccgc cgatggcgac
     1501 cgggccgatg acgtggctga tggtgtcggt gatcctggcc ggattggcct ggttgaccgt
     1561 cgagatatgg gtgttgaagt catcgatgta caggctcgca tcgagcttgc tgacgacgct
     1621 atggtcgaac tctccggcat aggcgaggcg gccctcggcg acctgcagcg gcgcgtagcg
     1681 cccgatcgag gtgggatagg gcggcaccga gccggcgatg cccgcgtcga cggtcgtcac
     1741 gcgcgcgctg acctcgaagc gctgcttcgc atcgggcatg tagcccagca ccaccccccc
     1801 gccgccggag cggtaatcgc tgttgggcac ggtcccggcg ggcgtatcgt aattgccgga
     1861 acgccggccc gtcacatagg cgcgcaggtc gaagccgctg cccgaggctt ccatggcgac
     1921 gtcgctttgc acgccgttgc cattgttccg gtaggtcacc gaagcctcgc cgccggtgac
     1981 ctggaaatca tggtcgaggt cgccatgggc gcgcttggtg acgaagttga tcaggccgcc
     2041 caggccgtcg ctgccgtaga gcgacgaggc gggtccgcga acgacttcca tctgctcgat
     2101 ttccgtcggg ctgatcagca tgaactgcag ggtgttgcgg ccgcggaagc ggtcgccatc
     2161 gatgaagacc ggcacgcgca tatcgttggt gttcaggccg cgcaggaaga ccgtgccgcc
     2221 gatgccgcca gtgcggttga cggtggcgcc cggcacctgg tcgaacaggt ccagcgtgct
     2281 cagcgggttg accatctcga tttcctgatg gtcgacgacc gtcacgggct gggtggcggt
     2341 gtgcctgatc tgggccggcg cagagtcgga gcccttgggc gcgatgcggc catcgtcggc
     2401 cgtatcgccg atgacggaga tggccggcag ctgcgcggcg gtttcctggg cgcggaccgt
     2461 ggcggcggcc agcaggtttg cgctgactag cgtggcgaac agatatctgg cttgcatagg
     2521 agggagggca tgatgaggaa ggggtcaggg ccggccggcg gccggagtgg agtctttcag
     2581 ccagcgcagg aacccgtgca ggaaccgcgt ggtggccggc atgaaatagc tgccgtggtg
     2641 gtacatcggg tcgagcgagg tcacgatcag gcggccggcg ccgccgcggt cgtgctcgta
     2701 cagcacggaa ccggcgccgg ccttgtcgat cagcgacacg gcgccggccg gcggctggaa
     2761 cgtgccgtgc tggtgccagg tggcatcggc caggccgatg tagcgaaaca gcggatggtc
     2821 ggcggcggcc agccgcaggc cggaatccgc gcccggctct ttccaccacc agaagttcac
     2881 ttcgcacggc gtccacgcca cgccgtcgag ccagcgctcg gcgccggttt cccccatggc
     2941 gaccagcgtt tttccggcgg ccaggaagcg ggcgaagcgc tcgcggtgcg gcgccagcag
     3001 gtcggggttg gtgcggcacg acacgatcag cgcatcgcag ccgcgcagcg cggcatcgtc
     3061 cagctcgcgc acgtagatcg tgcggtcgat gcaaggccgc agcgacgccg tgtggaaggt
     3121 gcggtggtgg tagtaggtgc ccgcgtccag cgcggcgatc accggttcgg ccgcgctcat
     3181 gtccgggcct ccagccaggc cagcagctgc ggcaccacgc gggcggcgct gtcgccgccg
     3241 cccgcgtaca tccagagatc gttgcccgaa tgcaccagca gccggccgcc gccgggcagc
     3301 tcgagcagcc agtccaccgg caaggcgtcc ggccccagcg tgtggagcac ccgcgcgcct
     3361 ggcgccggcg ggttgccgcc gcgcgcatag aagcccgcca cgccacggcg gaaagtcagg
     3421 tgctcgggat cgacgccctc gaaaatgggg tggtcttcgg cgcgatgcac gcgcaggccg
     3481 tccaggcccc ggtgggcgac gggctggaac ggccgcagcc agcgcaggaa cggataggcg
     3541 acgtggccgt tgaagaccag cgtgccgccg gacagcagaa agacttcgag gcgctcgcgc
     3601 tggccggcca ggaagcgctg gtccgcgtgc gcgggaacca gcaggctgcg ccagcgccgc
     3661 aggtcggcat gcgccaggtc gtagagcgcg agcggctcgg caggggcgtt cgggccgccg
     3721 gtcagcgcgg ccgggccgcg gcccggccag tggttcaggt aggcggtggc ggaaggcatg
     3781 gcgttcaccg gaacagggga atgacgccgc gcacgtcgcg gccatcgtgg cgcagcgcgg
     3841 cgacgtgcac gggcaagcgg tacaggcggc tgaggcgttc gtcggtgcac atcaggccgg
     3901 tcggcccggc ctcgcagccc tcgtcgtgca tcagcagggt ctggtcggcg atgtgctgcg
     3961 cgtgctgcgg gtggtgtgtc gagaacacga ccgccagccc gcgttccttg gccaggcggc
     4021 gcagcaccgc gagcacgatg tcctggttgc gcaggtccag cgccgccgtc ggctcgtcca
     4081 gcagcatgat cgggctctcg ctggccatgg cgcgcgcgat gcagaccagc tggcgctggc
     4141 cgccgctgag cgtctggaag gactgcgcgc tcaggtgttc gagctccacg gcgcgcaggc
     4201 aggcccgggc ggcgtggaga tcgcccgggc ccggcgaggc gtaccagcgc aggtgccggg
     4261 cccgccccat gctgaccatg tccagtgtgt cgtaggagaa cagcgtgtcg gtctgctgcg
     4321 gcacgtaggc ggcatggccg cccaggcgca cggtgccggc cgcggctgcc tgcagcccca
     4381 gcaccgtgcg cagaaaggtc gtcttgccgc gcccgttggg gccgaggacg gccaggattt
     4441 cgccagcccg cacccgcagc gacaggcctt cgagcaggat gcgcccaccc gcccgcaccg
     4501 ccagatcgtc gacttcaatc atggcccgga tccttgcgcc gcgcgcggcg cagcagcacc
     4561 acgaacagcg gcgcgcccaa tagcgcggtg atcacgccca gcgggatctc ggcgctggtc
     4621 aggctgcggg ccgccgtgtc gaccagcacc atgtacgcgc cgccgaccag cgcggcggcc
     4681 ggaagctgcc ggcgctggtc ggcgccgacg aacaggcgcg cgatatacgg cacgaccaat
     4741 ccgacccagc ccaccgtgcc ggaaacggcg acgctggccg atgtcaccag ggccgtgcag
     4801 gccagcagca cccagcgcag cggctcgacc gccaggccca gggcggcggc ctgctcgtcg
     4861 cccagcgaca gcacgttgat gcgaaaccgc agcgcataga acaaccccat gccggccagg
     4921 gcgggcacgg ccgccgccgc tagcttggcg taggacgcgg tggcgaagct gcccatcagc
     4981 cagaacacca tggcgggcag cgtgtcgttg gggtcggcga aatacgtgac catcgaggtc
     5041 agcgcactga aaaatccgct gctcaccacg ccggccagca ggagcatcag catggtggtg
     5101 cggcgctgga cccggccgag catgtagacg atggccaccg ctaccaagcc gccggcgaaa
     5161 gacataccca gcgtggcggt cagcgaggaa aacagcagga tggacaggca gccgccgaaa
     5221 gaggcgcccg acgaaatacc caggatctgc ggccccacca gcgggttgcg gaacatgccc
     5281 tgcaaggtgc tgccgcacat cgccaggccg gcgccgacca gcgccccgag cagcacgcgc
     5341 ggcaaccgca cctggaacag gatgcgccgc tcgacatcgc cgcccgccgc cagccagccg
     5401 tggccgccgg cgggcaggca ggcgtcgagc aacatggccg ccacgtgcga caagggcagg
     5461 gggtagcggc cgatgcacaa ggcgaccagc atcgaggccg gcagcgcggc gcccatcagc
     5521 agccagcgcc aagatagggc cggcggcacg ccggcgaaaa cgccgcgtcg ttcaatgaaa
     5581 gcgttcatag cccgccgacc cgtcgttcat cgcggtccgc aggaccgcat cgatgtcttg
     5641 ctcgctgggc gtatagccgt acagccgcgg gtaggcctgc gcgatcagcg cgcgcaatgg
     5701 ccagtcgaac ttgtccgggt gcagcatcac gctcagccac tgccagtaca ggggcgactc
     5761 ctggttgggc gggtcccaca gatagccgcc ctccggcacc ttgtagaccc gccggttgcg
     5821 cacggccggc acatccgcca ggagagggtc ctggtagatc gtctgcggcg tcagcgcgcg
     5881 ctcgaagttg ttcagcaaaa tgacctcggg cgcccacgcc aggatctgct ccacgtcgag
     5941 cggctgcgag ctcgtgaccg acgacgccac gttgcggccg ccggccagcg tgatgtcgtc
     6001 ctcgaaattg gtgttgcggc cggcggcgcg gtattgcgga aaacggcgcg ccagatagag
     6061 caccttcggc cgctcggccg gccccatcga atcggtcacg cgggcgatgg cggcgtggac
     6121 ttggtcgcgc cactgcagct gttgccgggc ctgcgcctgg cggtcgagcg acatccccat
     6181 cagacgtatc cattcccggg tgtccgcctc ggtgccgtag cgcagcgcgg caaccggcag
     6241 gcccgcgttg cgcagcggcg cgagcagatc gtctcccatg tggccccatt gccagacgag
     6301 gtcgggccgc atctgcagca gcgtctcgac gttgggcgtg aatcccgatc gggtgatgtc
     6361 cgagcgcacc ccggccgcct gggggaaaat ccgcgccagc aggccgtcgc gcatcagcgc
     6421 ataggcagtg ggatgcatgc cggccaggtg ggccgggccg ccgtcgagcg acatgaacag
     6481 cgagctggcc ggcatgggca ccgtcacggc atgcaccgtc ggccccgcca gggtgacctt
     6541 gttgccgagc atgtcggtga aggcgatcgg ttccgcatag gcggctcggc acagcagggc
     6601 caggcaggcc agggcggcgg cgcgcccgaa gggcagggat ttcaggggtg gggacatgca
     6661 tccaggccgt cgttgcggcc gtcctgtttt acatagataa taagaatcaa tctcaattga
     6721 gattctaggt tccccccagg caggctcaaa tgacttttgt caatccgatc cacaccaatg
     6781 cagcccatgg gctgcaagcg ctgcgcgcgc acccatggtt caaggaattg gacgagcggg
     6841 aattgacgcc gctggccgcg gccagccgct ggctcgccta cgccccggga cgggaaatct
     6901 ttgccgaagg gcacgcgatc gcgtcctgcc tgctggtggt ctcgggctgc gtgcaggggc
     6961 tgcgctacac cgccgatggc ggcgagaaaa tattcggcca ggtgcggccg ggcggcatgg
     7021 tttccgcgct gtcggtcttc atgcgcgaac cgcggcacct gcacagcgtg cgcgccgtcg
     7081 aggcgggcga gggctacctg ctcgatgccg cggctttccg gcggctgtgc gaggcgaacg
     7141 cgctcctggc ctgccgggtg ctgcggcata gcgccgacct cgtgcggcac cataccgacc
     7201 agatcgactg gctcacgtcc agttcggccg aggagcggtt ggccgagtat ttattgcggg
     7261 tgggcaagcc gcacgcccag gaaccggtcg agctgccgct cacgcacagc cagatcgcca
     7321 tcaagctggg tatgcgcgcc gaaaccttga gccgcttgct cgcgaaatgg cggcgccagg
     7381 gctggatctc cgaccagcgg ggcgcgctgc gcgtcctgga cgtcgcctat ctcaagcgtc
     7441 ttgccgaggc cggcaagacg ccgctttgac gcgcgtcttc gaccgcggca cgcggaaacg
     7501 cgcgcagcgc gtgggcgcgg cggcgatcag gcgaacggga aggccgtgcc gtaatcgccg
     7561 atgtggacca ggtgcgatac cgcgggcgca tcgcccgccc accggtgcag catgaaggcg
     7621 ggcggctcca ggtacgaggc cggcttcgca tccggttcga gccgcaaggc gatctgcgtc
     7681 gccgtgctgg ggcaggtcaa tacgggcgtg ccgcccagcc gcgcatgcat ggcgcggtga
     7741 acgtgcccgg caatgacccg atccacgtga tcgtgggcgc ggatgacatc ggcgaatgcc
     7801 tgcgcgtcat gcagcccata gcggtccagg taggggatgc ccgtcgcaaa gggcggatga
     7861 tgcatcgcga ccagggccgg gcgtccgcgg caagccgcca gctcccggtc cagccagccg
     7921 agctgcgcag tgctcagctc gccgtggtgc gcgtccggta cgctcgtatc cagcgcgatc
     7981 aaccggatcg cgcccacgtc catggcaaag tcgaacgcct cgccggacgg gatgtgcgga
     8041 tgatcccgga aagcggcccg cagggtatcg cgccggtcat gattgccggg cagaaacgcg
     8101 aaaggcagcc gcagcccagc cagcagcgct tgcagcatgg cgtactcgtc ctcagccccc
     8161 tggtcgacca gatccccggt gatcaagacc atgtccggcg caggcgccag gcggttcagc
     8221 gtctcgacgg cccgggcgaa catcgcattg gaatcgacca cgccttggta cagcacgcct
     8281 tggggccgca cgtggatatc cgaaatctgc gcaatcgtgg tcatcgtcga cgatcaaaat
     8341 ccatggaggg gcggcttggc gaaaatggcg gccatcgcga ctacggttgt ccgtcgccgc
     8401 tgccgatcga acccgtagcc aacgcaaggg aagcgatggc cgacagcgcc gcgctgtagc
     8461 tgtccgcata cgcattccgg gccagcagca tctggttctg cgccagcgtg acttccgtga
     8521 gcgaacccac gccgttgcgg taggtggcca gcgccgcgtc gtacgtcgtc tgcgaggcct
     8581 tgatcagcac cgccgccgct tcatgcgatg ccaggctcgt gcgcagggca ttctggccgg
     8641 cgacgatctg gcgcacggcc tcgtcccggg tgcgcgccag gcgcaactgc gcgctggccg
     8701 cgtcgttgtg cgcctgcatc agcacggccg aacgcaggcc gccgtcgtag atcggaatgg
     8761 taacgccgag gagcacggcg gagccggcgc ggtggttgtc caggttgacg gcggccggct
     8821 gctggccgac ggcggggatg gcggtgatcg ccgactggcc cgagctgtac gaggtcgacg
     8881 cggacagaaa gaccttgggc aggaattccg tctcggcggc cttgaccttg gcctgttcgg
     8941 cctgctgcgc cgcgtaggcg cccagcatgt cggggcggcg cgccagcgcg tcggccacca
     9001 gggtctcgac cgaggcgccg aggtccgacg gcagggcgcg gcgcggcagg ggcgcgatgc
     9061 gcggcttgga gagcggcgag atgcccaggg ccgaaatcag gcccaggtag gcatcgctct
     9121 cgacgcccag ggcggaggtc agcgcgaacc gggcctgggc caggttctgt tcggcctggg
     9181 ccgtttcgac gacggtgccc gcgccgtgct ggtagcgcgc ccgcgccgcc gccgccagcg
     9241 tgccggcgtt ggccagcgcc tgccgggcgt tggcggcgcg cgcgcgggcg gcgtcgtatt
     9301 ggtagtaggc aacgctcacc tcgtggatga gctgctggtg cacggcggtc aacgcgatat
     9361 tggcggcgat ggacgtttgc tgcgccgcct gtacgcgcgc gtcgcggccg ccgaaatcga
     9421 acagcagcca ttgcagcgtc agcgcggaaa ccgtgccgtg cgtgctggcg tcgtccgagg
     9481 cgctgcccag gagattgttc gagccgctgc cgtggcccgc ggcatagccg cccatggcgg
     9541 tggcggcgag gtagggcagg taggcgctct tggcgatgcc gaccgcgagc gcggcattgc
     9601 gcgcggcgtt ccaggcgatg cgagtgcgcg ggttggccga ctgggcgagg tcgatcagct
     9661 cgggcagggc gtagtcgcgc gagctgtcca cgtccgttgc gggcgggagc tgcgcgagct
     9721 tcttgttggc gggcagggtg tagccgccgc ccgcggcgtc gccggacaac gcgggcgggg
     9781 cggcgagaat ctcgccgtcg ctgttcgtct gcggatgcca ggggcgatcc ggccccgcgg
     9841 gcgcgaggtc cagcgacgcg gacgcgcagc cgcccaggcc gagcagggcc gccacgtaaa
     9901 ccaggcgcat gccatgggca agcgattcag gccggtgcat ggacggcggg ctccttgggc
     9961 atgaggtcgg cggtgatggc atcgatgccg cgcagctgcg cctgcagcgt ggcattgccg
    10021 ggcatgcgat cggcggccag gaacagcggc gcttcgagcg ccgtcagttc cgccagcgcg
    10081 cggcgccgcg ccgcgaccca gtcggcgccg gggcgcaccc aggagggctc gtaagggatc
    10141 aggccgaggt cttgcccgat ggcctggtgg cgcatcaggg cgtcggccgc ttgcgcgcgg
    10201 cgttcggccg cgtcgccaag ctgcgcgatg cggcgccatt gctgcagcag ggcggcgatg
    10261 gcctggtcga tgcggccggc gatgctgacc ggccagatcc gcgtgaacac caggtagacg
    10321 acgatattgc ccagcaggat gccgatggcg cggtcgcgcg cgatggtcag gtcgaagccc
    10381 ggcgacgaac cctggatgac gcagaggaag aaggcgaaag cgatctgcag gcccgcatag
    10441 gaaatgcgcg gcgagccgac cgccacccag gccgacgccc aggaggcggt gaacaccagc
    10501 agcatcagtt cgccgatcga tgacagcgaa ggcacgatga agaccatggc cgcggtgccg
    10561 gccagcgcgc ccaccaggca gccggccagg cgcagggcga gcttctgcac ggtttcggcg
    10621 gtggtgccca gcgagacgat gtagcaggtg atgaagcagg tgtggatgcc ctgccaatcg
    10681 agctgcgtgt agagcgcgta gcagaacatg gccgccgccg tggtcttcag ggcgtaagcc
    10741 agatgggcgg ggttgctgcg cgcgtcgggc agcatgaacc ccgccttgtg ccgcgccggt
    10801 tggggcgctt cttgcgcggc ggccggcggc gcctcggtga aatgcacgat cgcgtcgtgc
    10861 agatcggcca gggcggcctg ggccaggggc ggcaggccct gcactgcggg caggtcgagc
    10921 tggatgtcga cggggtagcc gccctgcgcc agcatgcccg ccatggcgtc gagcgtgtcg
    10981 gccaggggat gggcgatcgc atccggcagc ctggccgaag gcgccgtgtc gagcaggtcg
    11041 acggcgatca tgaccgtggt gctggcggcc gtcgcctgcc gcagcgccgc caggtcggcc
    11101 gcggtggcgc cgccggccag cttgagccag gattcgatct gccggttgcc ttcgcgcagg
    11161 cattgcgcga agtcgtcgcg cggcgtgtcc ggctgccgca gccgccgggc ggaggcggcc
    11221 aggcgtgcgg ccagccgcag gcccgccagc cggcgcggcg acggggcgag cagcagctgg
    11281 acgagcatcg ccaggcccac ggggatggcc gccatcagcc aggcgtagag cagttcgcgc
    11341 gtggcggcct cgcccagggg aacgaggccg agctcgtcca gcccgaagcc gatgatcatg
    11401 gcgaatatcg cgcccaccgg gcgcagcttg ctggccgagg tcaggtagag cagggcggcc
    11461 gacacccccg ccatgcagcc cagccgcaag accgggtagt cgatgctgaa caccgcgatc
    11521 agcatcacca ggccgatgac gagggtgacg aacagcagca tgatggcggg cagcagcacg
    11581 ccggtgacgc gctccggacg gatgaggaag aacacgatat agaccgatag cgaggcctcc
    11641 ggcgtgccgt aggcggcggt gaccaagacg gtcagcgcgc agatcagcgc cacgcgcagg
    11701 gacgtcgcca tgcggccggg gaagggcgcg agcagggcca ggaggtcgcg cggcgccaac
    11761 gcccgcgcct tatcggcagg cgggcccatg ccggacctcg acgacggcgc tggcgccgac
    11821 ccgcagcagc ttgctgtcgg cttcttccaa ttgcacccgc accgggaagc gctgggcgac
    11881 ccggacccag ttcaccgact tctgcaccag gggcagcgag ctcggcaggt tcaccctgtc
    11941 cgtatcgagc acgcccgcgc cgatgccgac gattttgccg ggcagggcga cgccgcggtc
    12001 tatcatcgaa tagaccgtcg cgcagtcgcc gggcgcgatg cggacaagct cggtctcgcg
    12061 aaagttggcc accgcgaacc attcgccggc gtcgatcagc gtgaagatgg actggctggg
    12121 cgccaccgtc tcgccggcga ggatcgacag gccggtgacg tagccgtcgt gcggcgcgta
    12181 caccaccgtg tcgtcgagcg cgtgccgggc gcgggccagg gcggcctcgc gcgcgtgcag
    12241 ggcggccagc gcgtcggcat cgtcgccgat ggtgctggac gcggtttggt gctgcgcctg
    12301 ggcctgccgc agcgataccg cggcatcctg ctgcgcgacc tgggcctgat cgtattgctg
    12361 cgccgggata tagccttgcg ccgacagcgg agccaggcgc tgcgtggtgc gggcggccag
    12421 cgcgtagttt tgctcggcgc gttgccgttg ctcgccggcg atggcggcat tggcgcgctc
    12481 ggcgatcagg gtcttgcgcc gggattcgag cgcggcccgg gccatctcga cctcggcctg
    12541 cgcctgggcc acggtgagcc gataaggcac cgggtcgatc tcgaacagga cgtcgccctt
    12601 ggcgacgcgc tggttttcgc gcaccgccag ccgcacgatg cgcccgccca ccggcgaggc
    12661 gatgtggacg tagtcggcgt ctatcgtggc gtcgtcggtg gaggggtagt tcgtgctgcg
    12721 gtgataggcg aaggccagcg ccgcgaggcc caggacgacg atgagcgcgg cgatggcgcg
    12781 gcccttgacg gagggttttc tgatgcccgc gagcttcatg ggatctggcc cgtgccgatg
    12841 ccccagacga gcaccccggc cacgaggccg atggcggtgc aaacggaaag cgggtaaggc
    12901 acggtttccg accagccggt catgacgaat agccggtgcc cggccagagc ggcggcaacg
    12961 ccggccaccg cgctcaacag ccaatgcgga aagtaagccc cgaacagggc gtacgatgga
    13021 gcgcccattt tgcgtttcct gccaagggag tggaagtgcg ggcaggatac cgccaatgcg
    13081 cggtttgggg aagccgccgg gaatcggcct ctcttgcgcg ggcaggccgg aaaaacgcgg
    13141 cggcggatcg ctcgcgcctt ggcactcggc agggtttaca cccattaatt ggatttctga
    13201 ttgaccgtta aggcctggaa ataagttcgg acatatttcc ttcgattttc gcggcatttc
    13261 ggtccggagc cgcgcccgcc ttcagttcaa cccctcggaa atccggtcta gccctatgtt
    13321 tgcgaatttt tccatcagag cgcgggtgtc gctcgcgttc ggactgcttg tggtgctgat
    13381 gctggtcgtc gccgcgctgg gcgaactcgg cgcgctgcgc ggcaagaatg ccttgcacga
    13441 tacctactcc gtccaactgg cctcggcagt ggcgctgggg gacagcaagt acaacctggc
    13501 catcgcgcgc gtggccatcg accgggcgct gctgggttcg aacgcggccg acctgccggg
    13561 cctggcgcag aaagcgcgcg actacctggc gacgtcgcaa aaagcctatg cgcgctacct
    13621 ggcgctgccc aagcgcagcg aggaagcgca actggccaag gccgtgtcga gcgattggga
    13681 caagctgctg gccggcgcga tcacgccggc gctgcaggcc ctgcaaaacg gcgacgcggc
    13741 gacggccaac cggttcacga tggaagtcat gccgccgctc tcgctgaacc tcaccaagag
    13801 cctggaccgg ctcaacgcct ggctcatgca gcaaggccag gacaattacg gccagttcca
    13861 gcagacgctg acgcgcatca gcctcgcagc gggcgtggcg ctggcgttcg gcgtgatggt
    13921 cgcgctggcg tgcacggtcg gcctgcaccg ggcgatttcc cgcccgctcg ggcatgcgct
    13981 ggaggtgtgc tcgtcgatgg cgcagggcaa cctcacgcag cggatcgagg tgcgcggccg
    14041 cgacgagatg agccagatga tgctcggcct gtcgcagatg caggcgggct tgcgcggcac
    14101 cgtgtcgacc atgagcgaga gctgcgaatc gatggccacg gcgacgcagc agatcgcctc
    14161 gggcaactcg gacctgtcgc ggcgcaccga gtcccaggcg gcggccctgc agcagaccgc
    14221 cgccagcatc gaggaactga ccgcgacggt gcggcagaac agcgggagcg ccagcagcgc
    14281 gagcgggctc gccaaccagg cgacaggcgt tgcccaaacc ggcggcgagg tgatggcgcg
    14341 cgtggtggcg accatgtcgg gcatccgcga acaatccacc aagatcgcca ccatcatcgg
    14401 caccatcgag tcgatctcgt tccagaccaa tatccttgcg ctgaacgcgg ccgtcgaggc
    14461 ggcgcgggcc ggcgaacagg gccgcggttt cgccgtggtg gcggccgagg tgcgcacgct
    14521 cgcgcagcgc tcggcgtcgg cggcgcggga gatcaagacc ctgatcgacg gcgcggtcgg
    14581 ccgcatcgcc gaaggttcgg gcctggtcga ctccgccggc cggacgatgg acgaaatcgt
    14641 ccagtccatc cagcgcgtca gcgggatcat ggacgaagtg gccaccgcgt cccgggagca
    14701 gggcgacggc atcgagcagg tcaaccaggc gatttcgcag atggacgagg ccacccagca
    14761 aaacgccgcg ctggtcgagg aaaccaccgc cgccgccgtg tcgctggccg agcaggccca
    14821 ggtgctgcgc gatgccgccg cgcgctttcg catttaacat gcggacctga cacgcgtatt
    14881 tcctcctgct tcgccatcgg gttgctgcat ggcggctttc ctatcctggg taagatggga
    14941 cgacaggcag tggttgtcac ggcctgccga taatgccgaa ggagagagaa tcatgaggat
    15001 ttacccagga ctcaagcttg tcgccgcgct cgcatgcggg gcggttgccg cgctgccgat
    15061 gtcggcagtc gcagccgaga cggcgtcagg agagctgctc ggcagcgacg gcgctgccca
    15121 cggaaccgtt caagtcacgg cggcgccggc aggcgtgctg ctgcgcatcg aagccaaggg
    15181 catggccccg ggctggcatg gcgtccactt ccacgagaag ggggattgcg gcgcgcccaa
    15241 attcaccagc gccggcgccc atgtccattc ggtcacgccg gtcgtgcatg gtttgctcaa
    15301 tgccaatgcg aacgatgcgg gcgatcttcc caatattttt gtcggggccg atggcgccat
    15361 gaccgtggaa ctgtattcga cgctcgtctc gctcaagccg ggcggcagcc gcccggcgct
    15421 gctcgacgcc gacggcgccg cgctcgtgat acacgccaat cccgacgact atcaaagcca
    15481 gccgatcggc ggctcgggcg accgggtcgc gtgcgccgtt ctccattgag cggccccgcg
    15541 tcacaaagtt tgcgtcaagc acggcaggcc tgacctgccg gccgggttcc cgctttcaac
    15601 gcatctggag aacgatgacg ttgctgctgg ccggattcgt ccgttcgcgc aggctttgca
    15661 cgtctgccgg cttcaccgcg ccgccctggt tgccccagga ggtgcggaca aagctcagga
    15721 cgtccgcgag ttcctggtcg gagagctggt tgcggaaaga cggcatgcgg taggcgtccg
    15781 ggacgccgtt cgcgacgatc cgcgccgacc cgttcaaggt cacgttgatg ctggatgcgt
    15841 tttctttcgc catggacgcc gcggacccgg ccagcggcgg tatccattgt ccctgtccgc
    15901 gcccctgcgc gccatggcag gcgctgcatc gcgccatgaa ggtctgcgcg ccgggtatct
    15961 gcagccgctg accgagggcc agggcgtcgt tcggcgccgt atcgggctgc cacggcggcc
    16021 catccttgcc gggtgtgccg ggcagcgact tcaggtagtg cgcaatcgcc ttcaaatcct
    16081 cgtccgacat gaattgcgtc gaattgttga acgcatcggc catcgagccg aaaacgacgg
    16141 catgcgagtt ccggcccgtc ttcaggaact gggcgatgtc ggcttcgctc cagcggccca
    16201 gccccgtgtt cgcatcgccc cgcaggctgg gcgcatacca gccgtcgagc agcgcgccgg
    16261 aaaggtagcg cgggtcgctg tcgtcgagcg ctatctcgtt catcgcgacg ctgcgcggcg
    16321 tatggcagct gccgcaatgg ccggcgccct ggacgagata ggcgcc
//
LOCUS       DASWPO010000271         8672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1760530, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000271 DASWPO010000000
VERSION     DASWPO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760530"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VGC69_14290"
     CDS             complement(<1..1623)
                     /locus_tag="VGC69_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0296494.1"
                     /translation="MIRASGLTLRRGTKVLLDGADFVVHPGERVGIVGKNGAGKSTLF
                     ALLTGELDADAGTLDLPAAWFIASVAQEIHADDRPAREFVIDGDTRLRDLQARRAALT
                     DDQGTQIAEVEAELAEAGAWDAASRAEQLLAGLGFKPTEWMNPVASFSGGWRMRLALA
                     RALMAPSDLLLLDEPTNHLDLDAMLWLEKWLAAYPGTVLLISHDTEFLDAVARAILHF
                     DHGKLNRYKGGYDDFITQRAERLRQTSIAHERQTREAARLQGFIDRFKAKASKAKQAQ
                     SRVKALARMETLAPLHAEAGIDIRIPSPDHMPDPLLVMEHMDAGYDAPILRDVTLMVR
                     AGARIGVLGVNGAGKSTLIKTLAEELAPLGGERRASRGLAIGYFHQHQLDMLDLDGTP
                     LQHLARLAPEVREQELRNYLGGFGFSGETVLSKVGPMSGGEKARLALSLIVWQKPNLL
                     LLDEPSNHLDVETREALATALAEFSGSMLLVSHDRHLLRTTVDSFWIVADGRVQEFEG
                     DLEDYRDWLSARNAAQRAAGRSEAMAAKATSSG"
     gene            complement(1620..2534)
                     /gene="prmB"
                     /locus_tag="VGC69_14295"
     CDS             complement(1620..2534)
                     /gene="prmB"
                     /locus_tag="VGC69_14295"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY0296495.1"
                     /translation="MAVSESRDALLTVRDLVRYGVSRLNAARVALGHGSDNAWDESVY
                     LVLHALHLPLDTLDPFLEARVLDGERAAVLGLIDRRVAERVPAAYLTHEAWLRGRRFY
                     VDERVIVPRSPIAELLDEGLSPWVADSGAVTRALDMCTGSGCLAILAALAFPNAQVDA
                     VDLSPDALAVARRNVDDYGLQDRLALHHGNLFDSLPIQAYDVIVCNPPYVNQSSMDAL
                     PPEYRHEPGMALGGGADGMDLVRRILDAAPRYLAPEGMLVLEIGHEAENFVAAFPQLE
                     PVWLETALTSDQIMLLTREQLAPSQLNP"
     gene            complement(2540..3679)
                     /gene="dapE"
                     /locus_tag="VGC69_14300"
     CDS             complement(2540..3679)
                     /gene="dapE"
                     /locus_tag="VGC69_14300"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY0296496.1"
                     /translation="MTTSAVLDLVRDLIARPSVTPDDIDCQQLITQRLAPAGFACETI
                     ERGGVTNLWARRGASGPLVVFAGHTDVVPPGPRDKWESDPFAPTERDGYLYGRGAADM
                     KSSIAAFIVASEEFVAAHPGHEGSIALLLTSDEEGPAVDGTVIVCEELKRRGVQLDYC
                     IVGEPTSGEKLGDTCKNGRRGSLSGKLTVRGVQGHVAYPHLARNPIHQLAPVLAEIVS
                     QEWDQGNEYFPPTTFQVSNLNSGTGATNVVPGEAVALFNFRFSTASTPESLKSRVHAI
                     LDRHGLEYDLDWGLGGEPFLTPRGSLTDALSKAIADETGVTTALSTTGGTSDGRFIAK
                     ICPQVIEFGPGNATIHKINERILLDDLVPLKNIYRRTLENLLVRG"
     gene            complement(3679..4500)
                     /gene="dapD"
                     /locus_tag="VGC69_14305"
     CDS             complement(3679..4500)
                     /gene="dapD"
                     /locus_tag="VGC69_14305"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0296497.1"
                     /translation="MTLDLQTAIDGAWEHRAGLSPFDASAEIREAVEHTLNALDVGRL
                     RVAEKVDGAWITHQWIKKAVLLSFRLQDNAVMGQSPLQFYDKVPLKFAEYGETAFKTG
                     GYRVVPPAVARRGCFIGKSVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKHVHLS
                     GGVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVVVEENSVLAMGTFLSQSTKILDR
                     ATGKISYGRIPSGSVVVSGSLPSADGTHSLYCAVIVKRVDAQTRAKTSINDLLRA"
     gene            complement(4556..5752)
                     /gene="dapC"
                     /locus_tag="VGC69_14310"
     CDS             complement(4556..5752)
                     /gene="dapC"
                     /locus_tag="VGC69_14310"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY0296498.1"
                     /translation="MNSRLDLLHPYPFQKLRALLAAAGAPPQDLAPIDLSIGEPKHAA
                     PECVLRALRENLQGLSVYPSTQGDARLREAIAQWLGRRYGIPAPDAGTQVLPVLGSRE
                     ALFAFTQVVLDPARGGKVVCPNPFYQIYEGATLLAGGEPYYLNADPARNFGGDWLAVP
                     EAVWRDTRLVFVCSPGNPAGNVMSLDDWRVLFDLSERHGFVIASDECYAEIYLDEANP
                     PLGALLAAHQLGRGDYARLMVFSSLSKRSNVPGLRSGFVAGDAALIAPFLRYRTYHGG
                     AMSPVVSAASIAAWSDEAHVRDNRRQYREKFDAVLPVLQPVLDVQAPQASFYLWARAP
                     GSDADFAARLYAATGVTVLPGSYLAREAHGINPGQGRLRLALVAEPAQCREAAERIAR
                     FAAAAL"
     gene            5799..5885
                     /locus_tag="VGC69_14315"
     tRNA            5799..5885
                     /locus_tag="VGC69_14315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5833..5835,aa:Leu,seq:tag)
     gene            6068..6388
                     /locus_tag="VGC69_14320"
     CDS             6068..6388
                     /locus_tag="VGC69_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296499.1"
                     /translation="MNILSSRLATLENGMSLSTTIDGESLTVYAVLGEPDFEIIPRIV
                     PVALVEAGAEIHAAVVDGVDQAQDQIDEVLDNVNPGDAVVFFCADENCYNAALDLLGL
                     PVDD"
     gene            6636..6839
                     /locus_tag="VGC69_14325"
     CDS             6636..6839
                     /locus_tag="VGC69_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY0296500.1"
                     /translation="METGVVKWFNAEKGYGFITPESGGKDLFAHFSEIQSQGFKTLEE
                     NQRVSFVTAMGPKGPQATKIQAL"
     gene            complement(6911..7462)
                     /locus_tag="VGC69_14330"
     CDS             complement(6911..7462)
                     /locus_tag="VGC69_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEY0296501.1"
                     /translation="MSSSVPRLKPLPAVIFSSRWLQLPLYLGLIVAQCVYVVQFIKEL
                     WHLVAHATTVGEQEIMLMVLGLIDVVMISNRLIMVIVGGYETFVSRLNLGDHPDHPEW
                     LGHVDASLLKVKLAMAIVGISSIHLLRTFIEASNLGGPSARVTSEGVMWQTIIHVVFI
                     LSALGIACVDRLTSAGEDHGKHH"
     gene            7657..>8672
                     /gene="tig"
                     /locus_tag="VGC69_14335"
     CDS             7657..>8672
                     /gene="tig"
                     /locus_tag="VGC69_14335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0296502.1"
                     /translation="MQPVVQTLSGLERRIDLAVSVADVEKEVQTQLKRVARTAKVQGF
                     RPGKAPMAMLERSHGPGIRYDVINSQIGRAFEQAVEEAKLRVAGAPNVEPKTEGVGED
                     TLAFTATFEVYPDVAVPDLSALSVARSTTEVTDAEVDKTIEVLRKQRAVHEPREDRAA
                     ADGDRVVLDFAGTIDGVPFEGGRAENFPFVLGQGRMLPEFEAAAQGLKAGETKVFPLK
                     FPDDYQGKEVAGKTAEFTITVREVAEGVLPAVDAEFAKQLGEADGNVDKLRADIRGNI
                     EREAKGRAEARTKNSVMDALVEAAKFDVPKSLVDSDVESRVAAAREDLKQRGVPNADS
                     MPIPP"
BASE COUNT         1436 a         2778 c         2984 g         1474 t
ORIGIN      
        1 gcctgacgag gtggccttgg cggccatcgc ttcggatctc ccggcggccc tttgcgcggc
       61 attgcgcgcc gacagccagt cccgataatc ctccaggtcg ccttcgaact cctgcacccg
      121 gccgtcggcc acgatccaga agctgtccac cgtggtgcgc agcaggtgcc ggtcgtgcga
      181 gaccagcagc atgctgcccg agaactcggc cagcgcggtg gccagggcct cgcgggtttc
      241 cacgtccagg tggttgctgg gttcgtcgag cagcagcagg ttgggctttt gccagacaat
      301 gagcgacagc gccaggcggg ctttttcgcc gccggacatg gggccgacct tgctcagtac
      361 cgtttcgccc gagaaaccga agccgcccag gtagttgcgc agctcctgct cgcgcacctc
      421 gggcgccagg cgcgccaggt gctgcagggg cgtgccgtcc aggtccagca tgtcgagctg
      481 gtgctggtgg aagtagccga tggccagtcc gcgcgaggcg cggcgttcgc cgcccagcgg
      541 cgccagctct tcggccaggg tcttgatcag cgtgctcttg cccgcgccgt tcacgcccag
      601 cacgccgatg cgcgcgccgg cgcgcaccat gagggtcacg tcgcgcagga tcggcgcatc
      661 gtagcccgcg tccatatgtt ccatcaccag cagcgggtcg ggcatgtgat cgggcgaggg
      721 gatgcggatg tcgatgccgg cttcggcgtg caggggcgcg agcgtctcca tgcgggccag
      781 ggccttgacc cggctctggg cctgcttggc cttcgaggcc ttggccttga atcggtcgat
      841 gaagccttgc agccgggcgg cttcgcgggt ctggcgttcg tgggcaatgc tggtctgccg
      901 caggcgctcg gcgcgctggg tgatgaaatc gtcgtagccc cccttgtagc gattcagctt
      961 gccgtggtcg aagtgcaaga tggcgcgcgc cacggcatcg aggaactcgg tgtcgtgcga
     1021 gatcagcagc acggtgccgg ggtaggcggc cagccacttc tccagccaca gcatggcgtc
     1081 caggtccaag tggttggtgg gttcgtccag cagcaacagg tcggaaggcg ccatcagcgc
     1141 gcgcgccagg gccaggcgca tgcgccagcc gcccgagaag ctcgccaccg ggttcatcca
     1201 ttcagtgggc ttgaagccca ggccggccag caattgttcg gcgcgcgagg cggcgtccca
     1261 ggcgccggcc tcggccagtt cggcctccac ctcggcgatc tgcgtgcctt ggtcgtcggt
     1321 caaggcggcg cgcctggcct gcaggtcgcg caggcgcgtg tcgccatcga tgacgaattc
     1381 gcgggcaggg cggtcgtcgg cgtggatctc ttgcgccacg ctggcgatga accaggcagc
     1441 gggcaggtcc agcgtgccgg catcggcgtc gagctcgccg gtcaagagcg cgaacagggt
     1501 cgatttgccc gcgccgttct tgcccacgat gcccacccgt tcgccggggt gaaccacgaa
     1561 atcggcgccg tcgagcagca ccttggtgcc gcggcgcaag gtcagtccgg aagcgcgtat
     1621 cacgggttta actgcgacgg ggccaactgt tcccgcgtga gcagcatgat ctggtcggac
     1681 gtgagggccg tctctagcca cacgggttcg agctgcggga aagccgcgac gaagttctcg
     1741 gcttcgtggc cgatttccag caccagcatg ccttccgggg ccaggtaacg cggcgcggcg
     1801 tcaaggatgc gccgcaccag gtccatgccg tcagccccgc cgcccagggc catcccgggc
     1861 tcgtggcggt attcgggcgg cagcgcgtcc atggacgact ggttcacata aggcggattg
     1921 cagacgatca cgtcataggc ctggatgggc agggagtcga acaaattgcc gtggtgcagg
     1981 gcaagacgat cctgcaggcc atagtcgtcc acgttgcggc gggccacggc cagcgcatcg
     2041 ggcgagaggt ccaccgcgtc gacctgcgcg ttgggaaagg ccagggcggc gagaatggcc
     2101 aggcagcccg atccggtgca catgtccagg gcgcgcgtga cggccccgga gtcggccacc
     2161 cagggcgaca ggccctcgtc gagcagttcg gcgatgggcg agcgcggcac gatgacgcgc
     2221 tcgtccacgt agaagcggcg gccgcgcagc caggcctcgt gcgtgagata ggcggccggc
     2281 acgcgctcgg ccacgcgccg gtcgatgagg cccagcaccg ccgcgcgctc gccgtccagc
     2341 acgcgggcct cgaggaacgg gtccagcgtg tccagcggca gatgcagcgc gtgcagcacc
     2401 aggtacacgg actcgtccca ggcattgtcc gagccgtggc ccagcgccac ccgggcggcg
     2461 ttcaggcgcg acacgccata gcgcaccagg tctcgcacgg tcagcagcgc atcgcgggat
     2521 tcggaaacag ccatgcccat cagcccctca ccagcagatt ttccagggtg cggcggtaga
     2581 tgttcttcag cggcaccaga tcgtcgagca ggatgcgctc gttgattttg tggatggtgg
     2641 cattgccggg gccgaattcg atcacctgcg ggcagatctt ggcgatgaag cgcccgtcgg
     2701 acgtgccgcc cgtggtcgag agtgccgtcg tcacgccggt ttcgtcggcg atcgccttgg
     2761 agagcgcatc ggtcagcgag ccgcgcggcg tgaggaaggg ctcgccgccc agtccccagt
     2821 ccaggtcgta ttccaggccg tggcggtcca ggatggcgtg cacgcgggac ttgaggctct
     2881 ccggcgtgct ggccgtggaa aagcggaagt tgaacagcgc caccgcttcg cccggcacca
     2941 cgttggtggc gccggtgccc gaattcaggt tggacacctg gaaggtcgtg ggcgggaagt
     3001 attcgttgcc ctggtcccat tcctgcgaca cgatctcggc cagcaccggc gccaactggt
     3061 ggatggggtt gcgcgccagg tgcgggtagg ccacgtggcc ttgcacgccg cggacggtga
     3121 gcttgcccga cagcgagccg cggcggccgt tcttgcaggt gtcgccgagc ttctcgcccg
     3181 aggtcggctc gcccacgatg caatagtcga gctgcacgcc gcgacgcttc agttcctcgc
     3241 agacgatcac ggtgccgtcc acggccgggc cttcctcgtc ggaggtcagc agcagggcga
     3301 tcgagccttc gtggccggga tgggccgcca cgaactcctc gctggccacg atgaacgcgg
     3361 cgatcgagct tttcatgtcg gccgcgccgc ggccgtacag gtatccgtca cgttcggtgg
     3421 gcgcgaaggg atcgctttcc catttgtcgc gcgggccggg cggcaccacg tcggtatgcc
     3481 cggcgaacac caccagcggg ccgctcgcgc cgcggcgcgc ccacaggttg gtgaccccgc
     3541 cgcgttcgat ggtttcgcag gcgaaacccg cgggggcgag ccgctgggtg atcaactgct
     3601 ggcagtcgat gtcgtccggc gtgaccgacg ggcgggcgat gaggtcgcgg accaggtcca
     3661 ggacggcgga tgtcgtcatc aagctctcag caggtcgttg atgctggtct tggcgcgggt
     3721 ctgcgcgtcc acgcgcttga cgatcaccgc gcagtacagg ctgtgcgtgc cgtcggccga
     3781 gggcagcgag cccgacacca ccaccgagcc cgaggggatg cggccgtagc tgatcttgcc
     3841 cgtggcgcgg tcgaggatct tggtgctctg cgacaggaag gtgcccatgg ccagcaccga
     3901 gttctcttcc acgaccacgc cttccaccac ttcggagcgc gcgccgatga agcagttgtc
     3961 ttcgatgatg gtggggttgg cctgcagggg ttccagcacg ccgccgatgc cgacgccgcc
     4021 cgacaggtgc acgtgcttgc cgatctgggc gcacgagccc acggtggccc aggtatcgac
     4081 catggtgcct tcgtcgacgt aggcgccgat gttgacgtac gagggcatca gcaccacgct
     4141 cttgccgatg aagcagccgc ggcgcgccac ggcgggcggc accacgcggt agccgccggt
     4201 cttgaaggcg gtctcgccgt actcggcgaa cttgagcggc accttgtcgt agaactgcag
     4261 gggcgactgg cccatcacgg cgttgtcctg caggcggaag gacagcagca cggccttctt
     4321 gatccattgg tgcgtgatcc aggcgccgtc gactttctcg gcaacgcgca ggcggcccac
     4381 gtccagcgcg ttcagggtgt gctcaacggc ttcgcggatc tcggcgctgg cgtcgaaggg
     4441 cgacaggccg gcgcggtgtt cccaggcgcc gtcgatggca gtttgcaggt ctagggtcat
     4501 ggtgtggact cgagtgtggg ttgaacggaa tgaatgaggg ttcgtgcggc tgcaatcaaa
     4561 gggccgcggc ggcaaatcgg gcaatgcgtt cggcagcctc ccggcactgc gcgggttcgg
     4621 ccaccagggc caggcgcaac cggccttgcc cggggttgat gccatgggcc tcgcgggcca
     4681 ggtagctgcc gggcaggacc gtcacgccgg tggcggcata caggcgggcc gcgaaatcgg
     4741 catcggagcc gggcgcgcgg gcccacaggt agaacgaagc ctgcggcgcc tgcacgtcga
     4801 gcacgggctg caggacaggc agcacggcgt cgaatttttc gcggtactgg cggcgattgt
     4861 cgcgcacatg ggcctcatcg gaccaggccg cgatgctggc ggccgagacg accgggctca
     4921 tcgcgccgcc atgatacgtg cgatagcgca gaaagggcgc gatgagcgcg gcgtcgcccg
     4981 ccacgaagcc cgagcgcagg cccggcacgt tggagcgctt ggacaggctg gaaaacacca
     5041 tcagccgggc atagtcgccg cggccgagct ggtgcgcggc cagcagggcc cccaggggcg
     5101 ggttcgcctc gtccaggtag atctcggcat agcactcgtc ggacgcgatc acgaagccat
     5161 ggcgctcgga caggtcgaac agcacgcgcc agtcatcgag cgacatgacg ttgccggccg
     5221 ggttgccggg cgagcagacg aacaccagcc gggtatcgcg ccagacggcc tcgggcacgg
     5281 ccagccagtc gccgccgaaa ttgcgggccg ggtcggcgtt gaggtagtag ggttcgccgc
     5341 cggccagcag ggtggcgccc tcgtagatct ggtagaaggg gttggggcac accaccttgc
     5401 cgccgcgggc cgggtccagc accacctgcg tgaaggcgaa cagcgcctcg cgcgaaccga
     5461 gcaccggtag cacctgggtg ccggcatccg gggcgggaat gccgtagcgg cgccccagcc
     5521 attgcgcgat ggcctcgcgc agccgcgcgt cgccctgggt cgagggatag acggacaggc
     5581 cctgcaggtt ttcccgcagg gcgcgcagca cgcactcggg cgcggcatgc ttgggctcgc
     5641 cgatcgacag gtcgatcggc gcaaggtctt gcggcggcgc tccggccgcg gccagcaggg
     5701 cgcgcagctt ttggaacggg taggggtgca ggaggtccag gcgggaattc atggggcagg
     5761 attttaacaa gcaagctaca ataatgagct tggcctttgc gagggtggcg aaattggtag
     5821 acgcaccagg tttaggtcct gacgccgtaa caggtgtagg ggttcgagtc ccctccctcg
     5881 caccagttga ctgcccccac tcgcacaaag tgcgagtcgc cccccgcggg ggctggtagg
     5941 ttcttgcccg cattgtttct gggttcttgc ctgccttgtt tctgggttct tgcccgtatt
     6001 ctctttgggt tcttgcccgt attgtctttg ggttcttgcc agcgccgggc ttttctttcc
     6061 acgacttatg aacatcctca gcagccggct cgccacgctt gaaaacggca tgagcctgtc
     6121 cacgacgatc gatggcgaat cgctcacggt gtatgccgtg ctgggcgagc ccgatttcga
     6181 gatcatcccg cgcatcgtgc cggtggcgct ggtcgaggcc ggggccgaga tccatgccgc
     6241 ggtggtcgac ggcgtcgatc aggcgcaaga ccagatcgac gaggtgctcg acaacgtcaa
     6301 tcccggcgac gccgtggtgt ttttctgcgc cgacgaaaat tgttacaatg cggcgctaga
     6361 cctgctggga ctacctgtcg acgactgatt tgcccctgcc tggaagggct tgggcaaaat
     6421 aacaacaaaa aagtaaaaaa ctgacgtttt gcttttattg gctggcgata ctacgctttg
     6481 tcatggattg cgtgctacca tgcgtcccct acggattggg gtgttgagtt cgtacagtgc
     6541 tatgcctttt ggcagccgtt tactggttcc ctccttgcta gatccgtggt cccgggcatg
     6601 tgccctctat tagcaatttg tttaggaaaa tcagtatgga aaccggcgtc gtcaagtggt
     6661 tcaacgcgga aaaaggttat ggcttcatca ccccggaatc gggtggcaag gacctgttcg
     6721 cgcacttttc ggaaattcaa tcgcagggct tcaagaccct ggaagaaaac cagcgcgtca
     6781 gcttcgtgac cgccatgggc cccaagggtc ctcaggccac caagatccaa gcgctgtaaa
     6841 tcacggcctt cgggcctgat ggcaaaaaag ccccgcaagg ggcttttttt attgcgcctc
     6901 gcggccgtgc tcagtgatgc ttgccgtggt cttcgcccgc gctggtcagg cggtcgacac
     6961 aggcgatgcc cagggccgac agaatgaaca ccacgtggat gatggtctgc cacatcactc
     7021 cttcgctggt gacgcgggcc gaagggccgc ccaggttgct ggcctcgatg aaggtgcgca
     7081 gcaggtggat ggatgaaatg cccacgatgg ccatggccag cttcaccttg agcaggctgg
     7141 catccacatg gccgagccac tcgggatggt cggggtggtc gcccaggttc aaccgcgata
     7201 caaaggtttc gtagccgccc acgatcacca tgatgagccg gttggagatc atcaccacgt
     7261 cgatcaggcc cagcaccatc agcatgattt cctgttcgcc gacggtggtg gcgtgggcaa
     7321 ccaggtgcca cagctccttg atgaactgca cgacatagac gcactgggct acgatcaggc
     7381 cgaggtatag cggcaactgc agccagcgcg agctgaaaat gacggccggt agcggtttga
     7441 ggcgtggaac ggaggagctc atgggcggtt tctgccgtaa aaatcgggac gttcgattct
     7501 agcaacccgg cccgttgcgc gtcggtcgcg gcggccggat gcagcccgtc cccatgccgc
     7561 cgggagggtt gccgggtttc cggtaaaatg agtggtttag ccgccgtgtt tcttcttgcg
     7621 gtctcttttc tcatctttcg caaataggtc tttgtaatgc agcctgtggt tcaaaccctc
     7681 tccggcctgg agcgccgtat tgatctggcc gtctctgtgg ccgacgtcga aaaagaagtg
     7741 cagacgcagc tcaagcgcgt ggctcgcacc gccaaggtcc agggctttcg tccgggcaag
     7801 gcgccgatgg ccatgctcga gcgcagccac ggccccggca tccgctacga cgtgatcaac
     7861 agccagatcg gccgcgcctt cgagcaggcc gtcgaagaag ccaagctgcg tgtcgccggc
     7921 gcgcccaacg tcgagcccaa gaccgaaggc gtgggcgagg acaccctggc tttcaccgcc
     7981 accttcgagg tctatcccga cgtggccgtg cccgacctgt cggcgctgtc ggtcgctcgc
     8041 agcaccaccg aagtcaccga tgccgaagtc gacaagacca tcgaagtgct gcgcaagcag
     8101 cgcgccgtgc acgagccccg cgaagaccgc gccgccgccg acggcgatcg tgtcgtgctg
     8161 gatttcgccg gcaccatcga cggcgtgccc ttcgaaggcg gccgcgccga gaacttcccc
     8221 ttcgtgctgg gccagggccg catgctgccc gagttcgaag ccgccgccca gggcctgaag
     8281 gcgggcgaga ccaaggtctt cccgctcaag ttccccgacg actaccaggg caaggaagtc
     8341 gccggcaaga ccgccgaatt caccatcacc gtccgtgagg tcgccgaagg cgtgctgccc
     8401 gcggtcgatg ccgagttcgc caagcagctg ggcgaggccg acggcaacgt cgacaagctg
     8461 cgcgccgaca ttcgcggcaa catcgaacgc gaggccaagg gccgcgccga agcccgcacc
     8521 aagaacagcg tcatggacgc cctggtcgag gccgccaagt tcgacgtgcc caagtccctg
     8581 gtcgactccg acgtggaaag ccgcgtcgcc gccgcccgcg aagacctcaa gcagcgcggc
     8641 gtgcccaatg ccgactccat gcccatcccg cc
//
LOCUS       DASWPO010000272         5586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2206960, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000272 DASWPO010000000
VERSION     DASWPO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206960"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1197)
                     /gene="potA"
                     /locus_tag="VGC69_14340"
     CDS             complement(82..1197)
                     /gene="potA"
                     /locus_tag="VGC69_14340"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEY0296503.1"
                     /translation="MIDSRQGTGQDEFVKIDEVVKIFGDVVAVRSVNLAVARNEIFAL
                     LGSSGSGKSTLLRMLAGFEQPSSGRILLDGEDITGVPPYRRPVNMMFQSYALFPHMTV
                     EANVAFGLKQEGIDRADIHDRVFEALALVQMAAYARRKPHQLSGGQQQRVALARSLVK
                     RPKLLLLDEPMSALDKQIRQKTQIELVKILEQVGVTCILVTHDQEEAMAMAHRLAVMT
                     EGRIVQCGTPQDVYAFPNSRFVAGFIGSTNLFEGAIVVDEPDHVAIESGDLPRRLVVS
                     HGVSEPLGMDVAVSIRPERIVVGREQPPGDFNWAHGIVSHMAWMGGYALYQVRLDSGK
                     MVEASVPSLALARMDAPGIGEEAFVSWDADSATVLSA"
     gene            complement(1202..2284)
                     /locus_tag="VGC69_14345"
     CDS             complement(1202..2284)
                     /locus_tag="VGC69_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0296504.1"
                     /translation="MAAVLAAALALAAGSARAEDPVVNVYNWAEYTAPDTLSGFEKAT
                     GIKVRYDVYDSNDTLQAKLLTGRSGYDVVVPSTHYAARQIQAGLFQKLDKSKIPNWQY
                     LDPDTMALVATVDPGNQYAIPWGYGTDGLGYNVTKVKQLLGDKADLGSWDTLFNPDNA
                     AKLKDCGISMLDEASQVFPAALHYLGKDPNSTNPDDYKAALDLLKKIRPYIRQFSSSG
                     YIDEMASGDLCMVYGYSGDVMIARRRAQEANKPYEINYFIPQGGAPAWFDVMVIPKDA
                     PHPEQALAFINYIETPQVHAAITNTMYYPNANKEARKYVTPDVANNPMIYPPADVTKT
                     LFVIKALPMDILRLQTRLWAELKSGR"
     gene            complement(2365..2793)
                     /locus_tag="VGC69_14350"
     CDS             complement(2365..2793)
                     /locus_tag="VGC69_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY0296505.1"
                     /translation="MTATTRVEPIFEPLDTSKTLFELLGGEPAIRALVDHFYDLMDME
                     ADFAALRAVHGPSLDNARNKLFWFLCGYFGGPDHYIQRFGHPRLRARHLPFSIGETER
                     DQWVACMGRAMLDQGVPEVLLERILHSFYGVADWMRNRAG"
     gene            complement(2848..5361)
                     /locus_tag="VGC69_14355"
     CDS             complement(2848..5361)
                     /locus_tag="VGC69_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0296506.1"
                     /translation="MTHPAPSRFSAWRLGLRMMLRDARAGELRLLILALVVAVAAITS
                     VGFLSDRVSQALVRDSAQMLGADLVLEADETLPPDFLAQARQRGLDTVSAVQFPSMAS
                     SADGAQLVSLKAVEPGYPLRGTLRVADQPYGPDAATREIPGPGTVWADPQLLSLLNVK
                     VGDSISLGDSRLRIAQVLAYEPDRGMQFISMSPHVLMNLADLPATNLVVNGSRVDYVL
                     MAAGQRGAVAAYQSWLGDHLQRGQRLASLESGRPEVRRTLDRAQRFLSLVALLSVLIA
                     AVAVALAAGRFMQRHRDGIAVMRCLGARQSQVTWLLLVEFGAVGLLSCLAGCVLGYGV
                     HQVLVWLLAGLIDTRLPAPSFVPALQGVLTGLLLLLGFALPSLARLRQVPPARVLRRD
                     LDALPARSLTGYLLGALGLALMIWWFAGDAKLAAVVAGGFLAAFLVFALAAWLCVLAL
                     GRVRGAVRGAPALRFALAGVVRRRLATITQVCALAVGLMALLLLTMTRTDLIAGWQRT
                     LPPDAPNRFLINVQDDQRDAVARQLTDAGLSRVTLLPMVRGRLITVNGKPVGPDDYDD
                     PQAKRLVDREFNLSYGGDLPASNSLVQGQWLKPDRTGEVSLETGLAERLRLHLGDKLV
                     FDVAGQQAEATVTSLRKVDWDSMQVNFFAILSPATLAGLPRSWITSFYLPPGRAAVLR
                     DLVARFPNLTIFDVAAILDQLQAVLAEVGKAVQLLFAFTLLAGMLVLAAALASTRDER
                     VRESAVLRALGATRGQLARAQRIELLAVGALAGLLAAAGATAVAWALATQVFDFTMSL
                     SLWPWLAGPAAGMLVAWTGGALALRGVLRAPPLVSLREA"
     gene            complement(5427..>5586)
                     /locus_tag="VGC69_14360"
     CDS             complement(5427..>5586)
                     /locus_tag="VGC69_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEY0296507.1"
                     /translation="LSEGEREDLGKQLQHAVKAYIGVTARVRVAESGFVERTLTGKAR
                     RVVDKRPR"
BASE COUNT          929 a         1870 c         1966 g          821 t
ORIGIN      
        1 ccagccagag atacggcggc aggatggcca aggtccggcc ggaaaagcgg cccggggacc
       61 tggagcggcc ggacatgggc ttcatgccga cagcaccgtg gcgctgtcgg cgtcccagct
      121 cacgaaggcc tcctcgccga tgcctggcgc gtccatgcgg gccagcgcca ggctgggcac
      181 gctggcctcg accatcttgc cggaatccag gcgcacctgg tagagcgcgt agccgcccat
      241 ccaggccatg tggctcacga tgccgtgggc ccagttgaag tcccccggcg gttgttcgcg
      301 cccgaccacg atgcgctcgg gccggatgga cacggccacg tccatgccca gcggctcgct
      361 cacgccgtgg ctgacgacca ggcggcgcgg caggtcgccg gactcgatgg cgacatggtc
      421 gggctcgtcg accacgatgg cgccctcgaa cagattggtc gagccgatga agccggccac
      481 gaaacgcgaa ttgggaaagg cgtagacgtc ctgcggggtg ccgcactgca cgatccggcc
      541 ttcggtcatc acggccagcc ggtgcgccat ggccatggcc tcttcctggt cgtgcgtgac
      601 caggatgcag gtcacgccca cctgctcgag gatcttgacc agctcgatct gggtcttctg
      661 gcggatctgc ttgtccagcg ccgacatggg ctcgtcgagc agcagcagct tgggacgctt
      721 gaccaggctg cgggcgagcg ccacgcgctg ctgctggccg cccgagagct ggtgcggctt
      781 gcggcgcgcg taggcggcca tctgcaccag cgccagggcc tcgaacacgc ggtcgtggat
      841 gtcggcgcgg tcgatgccct cctgcttgag gccgaaggcc acgttggcct cgaccgtcat
      901 gtgcggaaag agcgcgtagg actggaacat catgttgacg ggccggcggt acggcggcac
      961 gccggtgatg tcctcgccgt cgagcaggat gcggcccgaa ctgggctgct cgaagccggc
     1021 cagcatgcgc aacagcgtgg acttgccgct gcccgagctg cccaggagcg cgaagatctc
     1081 gttgcgggca accgccaggt tgaccgagcg cacggcgacc acgtcgccga aaatcttgac
     1141 gacctcgtcg atcttgacga attcgtcctg ccccgtgccc tggcggctat cgatcatccg
     1201 cttatcgtcc ggatttcagt tccgcccaca ggcgggtttg caggcgcagg atgtccatgg
     1261 gcagggcctt gatgacgaac agggtcttgg tgacgtcggc cggcgggtag atcatggggt
     1321 tgttggccac atcgggggtc acgtacttgc gcgcctcttt gttggcgttg gggtagtaca
     1381 tggtgttggt gatggccgcg tgcacctgcg gggtctcgat gtagttgatg aaggccagcg
     1441 cctgctcggg gtgcggggcg tccttgggaa tgaccatcac gtcgaaccag gccggcgcgc
     1501 cgccctgggg gatgaagtag ttgatctcgt agggcttgtt cgcttcctgg gcgcggcggc
     1561 gggcgatcat cacgtcgccc gaatagccgt agaccatgca caggtcgccc gaggccattt
     1621 cgtcgatata gcccgaggag ctgaactggc ggatgtaggg gcggattttc ttcagcaggt
     1681 ccagcgccgc cttgtagtcg tcggggttgg tgctgttggg atccttgccc aggtagtgca
     1741 gcgccgccgg gaacacctgg ctggcctcgt cgagcatcga gatgccgcaa tccttgagct
     1801 tggcggcgtt gtcgggattg aagagcgtgt cccagctgcc gagatcggcc ttgtctccca
     1861 ggagctgctt gaccttggtg acgttgtagc ccaggccgtc ggtgccgtag ccccagggta
     1921 tggcgtactg gttgcccggg tcgaccgtgg ccaccagggc catggtgtcg gggtcgaggt
     1981 actgccagtt ggggatcttg gacttgtcca gcttctggaa caggcccgcc tggatctggc
     2041 gcgcggcgta gtgggtggac ggcaccacca cgtcgtagcc cgaacgcccc gtcagcagct
     2101 tggcctgcag ggtgtcgttg ctgtcgtaga cgtcatagcg caccttgatg ccggtggcct
     2161 tttcgaaccc ggagagggtg tcgggcgcgg tgtactcggc ccagttgtag acgttgacga
     2221 cggggtcttc ggcacgggcg ctgcccgcgg ccagcgccaa ggccgccgcc aggactgccg
     2281 ccatcgtgtg ttgtccgagc ttcatgccct ctcccctgcc tggatcttga gcccaggatt
     2341 ttccgaaagg cgaaatgata aagcctatcc ggcccggttg cgcatccagt cggccacccc
     2401 gtagaacgaa tgcaggatgc gctcgagcag cacctcgggc acgccctggt cgagcatggc
     2461 gcggcccatg caggccaccc actggtcgcg ctcggtttcg cctatggaaa acggcaggtg
     2521 gcgcgcccgc aggcgcggat ggccgaaacg ctggatgtag tggtcgggcc cgccgaaata
     2581 gccgcacagg aaccagaaca gcttgttgcg cgcattgtcc aggctggggc cgtgcacggc
     2641 gcgcagcgcg gcgaaatcgg cctccatgtc catcaggtcg tagaaatgat cgaccagggc
     2701 gcggatggcg ggctcgccgc ccagcagctc gaacagggtc ttgctggtgt ccaggggctc
     2761 gaagatcggt tcgacgcggg tggtcgcggt catggaatac tcgtctaaac gggaaaggcc
     2821 cggaatcttg tctgcctggg gaggcgttca ggcttcccgc aagcttacca agggtggcgc
     2881 gcgcaggacg ccgcgcaggg ccagggctcc ccccgtccag gccaccagca tgccggccgc
     2941 cgggcctgcc agccagggcc agaggctcag gctcatcgtg aaatcgaata cttgcgtggc
     3001 cagcgcccag gccacggccg tggcccccgc cgcggccagc aggccggcca gcgcccccac
     3061 cgccaacagc tcgatgcgct gggcacgggc caactgcccg cgggtggcgc ccagggcgcg
     3121 cagcaccgcc gactcgcgca cgcgctcgtc gcgcgtggac gccagcgcgg cggccaggac
     3181 cagcatgccc gccagcagcg tgaaggcgaa cagcagttgt actgccttgc ccacctcggc
     3241 cagtacggcc tgcaattgat ccaggatggc ggccacgtcg aagatcgtca ggttgggaaa
     3301 gcgggcgacc agatcgcgca gcaccgcggc gcggcccggc ggcaggtaga acgaggtgat
     3361 ccagctgcgc ggcaggccgg ccagcgtggc cggcgacagg atggcgaaga aattgacctg
     3421 catcgaatcc caatccacct tgcgcaggct ggtcaccgtg gcctcggcct gctgcccggc
     3481 cacgtcgaag accagcttgt cgcccaggtg caggcgcagg cgctcggcca agccggtttc
     3541 caatgacacc tcgccggtcc tgtccggctt gagccactgc ccttgcacca ggctgttgct
     3601 ggcgggcaag tcgccgccat aggaaagatt gaactcgcgg tcgaccaggc gcttggcctg
     3661 cggatcgtcg tagtcgtccg gcccgaccgg cttgccgttg acggtgatca gccggccgcg
     3721 caccatgggc aggagcgtga cccgggacag gccggcgtcg gtgagctgcc gcgccacggc
     3781 atcgcgctgg tcgtcctgca cattgatgag gaaccggttg ggcgcgtcgg gcggcaaggt
     3841 gcgctgccag ccggcgatca ggtcggtgcg ggtcatggtg agcaggagca aggccatcag
     3901 ccccaccgcc agcgcgcaga cctgcgtgat ggtggccagc cgccggcgca ccacgcccgc
     3961 cagggcaaag cgcaaggcgg gcgccccccg cacggcgccg cgcacccggc ccagggccag
     4021 cacgcacagc caggccgcca gcgcgaacac caggaaggcg gccagaaagc ccccggccac
     4081 cacggccgcc agcttggcgt cgccggcgaa ccaccagatc atcaaggcca gccccagcgc
     4141 ccccaggaga tacccggtca ggctgcgggc cggcagggca tcgaggtcgc ggcgcagcac
     4201 gcgggccggc ggcacctggc gcagccgcgc cagcgagggc aaggcaaaac ccaggagcag
     4261 cagcaggccg gtcaacacgc cctgcagggc cggcacgaag gacggcgccg gcaagcgggt
     4321 atcgataagg ccggccagca gccacaccag cacctggtgc acgccatagc ccagcacgca
     4381 gcccgccagg caggacagca ggcccaccgc gccgaactcg accagcagca gccacgtgac
     4441 ctgcgactgc cgggcgccca ggcagcgcat gacggcaatg ccgtcgcggt gccgctgcat
     4501 gaagcggccg gcggccagcg ccacggccac cgcggcgatc agcacggaca acagggccac
     4561 gagcgagagg aaacgctgcg cgcgatcgag cgtgcggcgc acttccgggc gccccgactc
     4621 caggctggcg agcctctgcc cgcgctgcag atggtcgccc agccacgatt gataagccgc
     4681 caccgccccg cgctggccgg ccgccatcaa aacgtaatcg acccggctgc cgttgaccac
     4741 caggttggtg gccggcaggt cggccaggtt catcagcaca tgcggcgaca tactgatgaa
     4801 ctgcatgccg cggtcgggct cgtaggccag cacctgggcg atgcgcaggc gcgaatcgcc
     4861 caggctgatg ctgtcgccca ccttgacatt gagcagcgag agcaattgcg gatcggccca
     4921 gacggtgccg ggacccggta tctcgcgggt ggcggcatcg gggccgtagg gctggtcggc
     4981 cacccgcagc gtgccgcgca gcggatagcc cggctcgacc gccttgagcg acaccagctg
     5041 ggcaccgtcg gcgctgctcg ccatggaagg aaactgcacc gccgaaaccg tgtccaggcc
     5101 acgctggcgg gcctgggcga gaaagtcggg cggcagggtc tcgtcggcct cgagcacgag
     5161 gtcggcgccc agcatctggg cggaatcgcg caccagcgcc tgcgagacgc ggtcggacag
     5221 aaagccgacg ctggtgatcg ccgccacggc caccaccagg gccaggatga gcaggcgcag
     5281 ttcgccggcg cgggcgtcgc gcagcatcat gcgcaggccg aggcgccagg cggagaatcg
     5341 tgagggggcg ggatgggtca tacacgcatg gtaaccaatc ccgcaggccg aaagccggcc
     5401 agcgcaacag actgtcgcaa agaccgtcag cgcggccttt tgtcaaccac gcggcgggcc
     5461 ttgccggtga gggtgcgttc gacaaagccg ctctcggcca cgcgcacgcg ggcggtcacg
     5521 ccgatatagg ccttcaccgc gtgctgcagc tgcttgccca ggtcctcgcg ctcgccctcc
     5581 gacagg
//
LOCUS       DASWPO010000273         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3099040, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000273 DASWPO010000000
VERSION     DASWPO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3099040"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGC69_14365"
     CDS             complement(<1..306)
                     /locus_tag="VGC69_14365"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0296508.1"
                     /translation="MSSMPKIIYTLTDEAPFLATQSLLPIVDAFTHTAGIVVETRDIS
                     LAGRILAQFPDLLNAGQKIADDLTELGALATTPEANIIKLPNISASMPQLKAAIKELQ
                     "
     gene            601..1593
                     /locus_tag="VGC69_14370"
     CDS             601..1593
                     /locus_tag="VGC69_14370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY0296509.1"
                     /translation="MNLPNPAIRLKNAWRSSHPWIFQKLVDKPPQKPKPGTIVDVFGV
                     DNDWVGRGFYNGHSRIAVRILETDPDVAVDQDWFNRKIAQAVALRREVLKLDEVSDAW
                     RVVHAEGDGLSGLVVDRYGDLIVVEFFAAGMFRHREWIFEALRAQFPGCRFHVFAEEH
                     VQKQESFDFHSTSGTEAEVITEYGVKFRADPAGAHKTGFFADQRENRQWLSQHAAGKT
                     VLDLCCNTGGFAVYAAARGATEVVGVDIDEDVIAIAKGNARLNGVRPRFVQADLFPYL
                     RDAAARGERYDMVILDPAKMTRDREQVIAALKKYLDMNKLALGVVKPGGLLATF"
BASE COUNT          313 a          537 c          580 g          303 t
ORIGIN      
        1 ctgcagctcc ttgatcgcgg ccttgagctg cggcatcgag gcggaaatat tggggagctt
       61 gatgatgttg gcttccggcg tggtcgccag cgcgcccaac tcggtcaggt cgtcggcgat
      121 cttctggccg gcgttcaaca ggtccgggaa ctgggccagg atgcggcccg ccagggaaat
      181 gtcgcgggtt tccacgacga tgccggcggt gtgggtgaag gcatcgacga tgggcagcag
      241 cgactgggtg gcaaggaaag gagcttcgtc ggtcagcgta tagatgatct tgggcatgct
      301 agacattgcg ggatctgtct ctcttgcgga tgcgggaatg tgaagcgcgg agcacgccgc
      361 atgcggatcg ggagacgacg cgcgatggac gtgccgctgg cgtcaaaacc gggcctcaag
      421 catgttcctt gccgaggcgg cggccgtgag cagccaagcg cgccattgtc gcgactaaag
      481 ggtggcaggg caaagctgcg caggcgtatg gcgcaggttg ctgtgttgca acggcttttt
      541 cttggcgatt tggttgccgg ggaaagcatc aaaacccgcg caggcttcta caataggccg
      601 atgaatctgc ccaaccccgc catccgcctg aagaacgcct ggcgttccag ccatccttgg
      661 atcttccaga aactggtgga caagccaccg cagaagccca aacccggcac gatcgtcgat
      721 gtgttcggcg tggacaacga ctgggtgggc cgcggcttct acaacggcca ctcgcgcatc
      781 gccgtgcgca tcctggagac cgacccggac gtggccgtgg accaggactg gttcaaccgc
      841 aagatcgccc aggccgtggc attgcgccgc gaggtgctca agctggacga ggtttccgac
      901 gcctggcgcg tggtgcatgc cgaaggcgac ggcctgtccg gcctggtggt ggaccgctac
      961 ggcgacctga tcgtggtgga gttcttcgcc gcaggcatgt tccgccatcg cgaatggatc
     1021 tttgaggcgc tgcgcgcgca attcccgggg tgccgcttcc atgtgttcgc cgaggaacat
     1081 gtgcagaagc aggagagctt cgatttccac agcacctccg gcaccgaggc cgaggtcatc
     1141 accgagtacg gcgtgaagtt ccgcgccgat ccggccggcg cgcacaagac cggcttcttt
     1201 gccgaccagc gcgagaaccg ccagtggctg agccagcacg cagcaggcaa gaccgtgctg
     1261 gacctgtgct gcaacaccgg cggctttgcc gtgtacgcgg ccgcacgtgg cgccaccgag
     1321 gtggtcggcg tggacatcga cgaggacgtg atcgccatcg ccaagggcaa tgccaggctc
     1381 aatggcgtgc ggcccaggtt cgtccaggcc gacctgttcc catacctgcg cgatgccgcc
     1441 gcccgtggcg agcgctacga catggtgatc ctggatccag ccaagatgac ccgcgaccgc
     1501 gagcaggtca tcgccgcgct caagaaatac ctggacatga acaagctggc cctgggcgtg
     1561 gtcaagcccg gcggcctgtt agccacgttc taatgcaccg gcctggtgac cgaggaccag
     1621 ttcctggaca tgctgcgtcg cgcggcctac ttctccggcc gcaccatcca gatcctcaag
     1681 gtttccggcg ccggcccaga ccatccgttc atggcccacg tccaggaatc gcg
//
LOCUS       DASWPO010000274        13399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1582604, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000274 DASWPO010000000
VERSION     DASWPO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582604"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGC69_14375"
     CDS             <1..298
                     /locus_tag="VGC69_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0296510.1"
                     /translation="AVGARFSADAMRRAQALTWDATAKIAELVRPGMRESEAISAGKA
                     LLAALGMERIWHPVQIRFGHNTLRTFGEPSQDDPVLGADDIYSRWSRTIGAPSG"
     gene            343..528
                     /locus_tag="VGC69_14380"
     CDS             343..528
                     /locus_tag="VGC69_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296511.1"
                     /translation="MGWKLNLDMKGHRVGDFPHAIYKPGKLADLADTPSEGLWILEIQ
                     IAHPTRPFGAFYEDLLQ"
     gene            complement(578..901)
                     /locus_tag="VGC69_14385"
     CDS             complement(578..901)
                     /locus_tag="VGC69_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0296512.1"
                     /translation="MSPMPLDVIHPGEILLEDFMKPMQITARRLAADIDVPPSRISEI
                     VHGKRPVTADTALRLSIFFNMEIRFWLNLQTEYDLRKVTRELKDTLLTRIRRYEAPAG
                     GSASG"
     gene            complement(914..1195)
                     /locus_tag="VGC69_14390"
     CDS             complement(914..1195)
                     /locus_tag="VGC69_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0296513.1"
                     /translation="MIVSFACLDTETLYLGLPVPRFRHIERVALRKLLQISAATELRT
                     LQVPPGNRLETLRGDRQGQYSIRINDQWRVCFTWHPGEGAHDVEIVDYH"
     gene            1370..1852
                     /locus_tag="VGC69_14395"
     CDS             1370..1852
                     /locus_tag="VGC69_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0296514.1"
                     /translation="MNDSTTLSTDAAPAAGQDAPLLLDDQLCFALHSTSLAMNKVYRK
                     LLGKLGLTYPQYLVMLVLWERDGLILSDIGKRLFLDSATLTPLLKRLEAAGLIRRTRA
                     VRDERQVVIDLTPEGRALRKQAAEVPYQVFCATACTPQALQQLKGELELLRGHLAEYE
                     "
     gene            2028..2453
                     /locus_tag="VGC69_14400"
     CDS             2028..2453
                     /locus_tag="VGC69_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEY0296515.1"
                     /translation="MSLEKVLYRAQAQATGGRDGRAVSSDNVLDVKLTTPRELGGAGD
                     KGTNPEQLFAAGYSACFLGAMKFVAMHDKIAIPSDVSVQGNVGIGPLPTGFGIEVELK
                     ISLPGMDHAAAQALVDKAHIVCPYSNATRGNIDVTLTLL"
     gene            2648..3589
                     /locus_tag="VGC69_14405"
     CDS             2648..3589
                     /locus_tag="VGC69_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296516.1"
                     /translation="MQPVISVQGVSKTYASGHQALKNINLDIRQGEIFALLGPNGAGK
                     TTLIGIICGIVNASAGRVLADGHDIVADYRAARQTIGLVPQELTTDAFESVWSTVSFS
                     RGLFGMPANPAHIEKVLRDLSLWEKKDERIRALSGGMKRRVMIAKALSHEPRILFLDE
                     PTAGVDVELRRSMWEMVRHLRENGVTIILTTHYIEEAQEMADRVGVIRKGEIILVEEK
                     AALMRKMGRKQLTLSLAAPLPAIPDALRGEPLELSADGLKLVYTYDQHLEGSGSIAGL
                     LARVGQAGLVFNDLQSSESSLEEIFVSLVNEQQGKTQ"
     gene            3586..4347
                     /locus_tag="VGC69_14410"
     CDS             3586..4347
                     /locus_tag="VGC69_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296517.1"
                     /translation="MNWYGVRAIYLFEMDRTWRTVMQSVASPVISTALYFVVFGSAIG
                     SRMVQIDGVSYGAFIVPGMVMLSLLTQSTSNASFGIYMPRFSGTIYEILSAPVSFVEI
                     VMGYVGAAASKSIVLGCIMLLTARVFVEFHILHPVWMAGFLVLTSLAFSLFGFIIGVW
                     ADGFEKLQVIPLMILTPLTFLGGTFYSIKMLPPFWQKVTLINPVVYLINGFRWSFYGV
                     ADVDVGVSLGMTLVFLLVCLVIIRWIFHTGYRMKQ"
     gene            4478..4825
                     /locus_tag="VGC69_14415"
     CDS             4478..4825
                     /locus_tag="VGC69_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296518.1"
                     /translation="MPVWLPILKASLPYLTQIVTTAIPVFTSRSAGKASGKPLPDEVV
                     GRQIAELQEAVTQNAESVHTLASKLQDTLQGIDFAANGLQSQLASLRRLARTALVVAV
                     ASLCVAAWALTRA"
     gene            4829..5044
                     /locus_tag="VGC69_14420"
     CDS             4829..5044
                     /locus_tag="VGC69_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEY0296519.1"
                     /translation="MPEENDIERRLIQLEVKAGFADDLLDQLNHTIFRQQQQMDLLRR
                     ELAELRRQMPASPGQPGSLRDELPPHY"
     gene            5054..5635
                     /locus_tag="VGC69_14425"
     CDS             5054..5635
                     /locus_tag="VGC69_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY0296520.1"
                     /translation="MQSAALGAGKPTPTSWSLFLIFTRIGLTSFGGGLSGWLMREFVQ
                     SRQWMDEEEFLNGLAISQALPGVNVTNMSIWIGYHLAGARGAAASLSGILLPPAVFAV
                     LLSVLFALLSGYPLVHVWLAGAAAAAIGLSLSMAITAVRRVRRRVLPLVFMVLVFVMV
                     AILRLSLVWVVLGLGPISVALEYLRLRRAGKQA"
     gene            5635..6168
                     /locus_tag="VGC69_14430"
     CDS             5635..6168
                     /locus_tag="VGC69_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY0296521.1"
                     /translation="MSALWGLFSTFVPISLVTVGGGQATVAEIQRQVVDTHHWLTQAQ
                     FLNAFAITRMAPGPGSLLVTLIGWQVAGFWGAVVATAAMFGPTAILLFTIAYFWRRQR
                     GANWQLALESGLRPIAAGMMAAAVYVLMSTLEGGWMAQAIALISTGLVMFTRVNALLL
                     VLGGGVALILCQMSGLA"
     gene            6199..8004
                     /locus_tag="VGC69_14435"
     CDS             6199..8004
                     /locus_tag="VGC69_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017403922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY0296522.1"
                     /translation="MTRGTDLDWNAQLSASLVWLAETFCIVIVVAAIAAAIFARSTKW
                     GRQFWRLAWPYFTPRRSWRPLAMVCLLVFFSLFGVRMQVLFSFWYNGFYSALQALDQN
                     AFWRFMGLFAVLAAVHVVRALTAYIIGQAFDIRWKIWLNDRLTQDWLGKRAYYRGQFV
                     DHAVDNPDQRIQQDVTLFIEGSRTLAMGVISSAVSLVAFTAILWSLSAPLTLMGVEIP
                     RAMVFLVYVYVVVATWLAFRIGRPLIRLSFLAEQLTANFRYALMRLRENAENVAFYQG
                     EDVERGTLFTRFKAYIANQWALIFRTLRFDGFNLVISQVAVVFPFLLQAPRFFSGALK
                     LGDVMQTSQAFGQVQDALSFFRTSYDSFAQYRATLDRLDGFLDANSAARELPAVQSAE
                     QAAGLRLEGLSVTRPDGQVLLRDLNLQLQPGQSVLIKGPSGSGKTTLLRAVAGLWPYA
                     QGKVARPLGAQSLFLSQRPYLPLGDLRGAVAYPSQADPADDARLQAALRAVNLGHLTG
                     KLGEVAEWSRILSIGEQQRVAFARVLVNQPKVIFLDEATSATDEGLEHALYTQLRASL
                     PESVLVSVGHRGTLAAFHSHRLELDGVGGWQVAAI"
     gene            complement(8066..9334)
                     /locus_tag="VGC69_14440"
     CDS             complement(8066..9334)
                     /locus_tag="VGC69_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY0296523.1"
                     /translation="MSATVTTSLAGATLTGTREQGICCYRHLPYAQAPVGELRLRPPR
                     PLTLAGAIDATQAGPVAPQLPSRLREVMGDFEASQSEDCLHLTVWTPGADANRRPVVI
                     WMHGGAWQSGGGAIDWYDGARLAREGDVVVVGINYRLAALGWLYLPGVAANLGLLDME
                     LAIEWVRRHIAALGGDPERITVMGQSAGGFNTALLLTREPQFTRAILQSASIGRGCRS
                     AAQALALTEVFMDAVGARDLDDLQALPCQALLQAQQAPAVLQALKDEGGARSLFCPVA
                     DGDVLPLDVDARLPEASGRADVLIGYTHDEMAAFPGGGTDEASHALGMRIFGTPAHQW
                     AQSAQEHGRQAWLYRMDLGPTDRYGACHCIELPFVFGTLETFAGAPMLHGMDPAHGAG
                     LSDAMRRDWIAFIRGGAPYPEPAPALHRYA"
     gene            complement(9455..9667)
                     /locus_tag="VGC69_14445"
     CDS             complement(9455..9667)
                     /locus_tag="VGC69_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY0296524.1"
                     /translation="MFPEFQDQITQLKSDNPHFARMMQKHGDLDLLIQNIETGRATGS
                     LLDLEKLKKEKLYIKDQIYALLRSVH"
     gene            complement(9767..10417)
                     /locus_tag="VGC69_14450"
     CDS             complement(9767..10417)
                     /locus_tag="VGC69_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296525.1"
                     /translation="MTSSLAQPISETLAAGVQFKVLAHVFPVLRHEVIAPVSNAGLAV
                     AMLRHGMHGTDSHQEGLINELESMLLESTAEVRRLGDWLEGTGATLPLDHLLELCRKL
                     LFGHLLRAARQIDLPESCPVLDLPEFGSRYVLLAWFLSLLDHLPHDDVLHIEMGGERH
                     LVARPSTPRGFAVPPATPGRIAMDEVQCLAEHHGWQCRFDDQSWSLKLPSGTVLPG"
     gene            complement(10515..12311)
                     /locus_tag="VGC69_14455"
     CDS             complement(10515..12311)
                     /locus_tag="VGC69_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HEY0296526.1"
                     /translation="MILLDTCHPRTLAAWLAEYTRPGHRGSRLEGWLFESAEARRAAE
                     ARLAQAGVIARLRSAYKPLLHYFLEEADLQGLRLAVVRYPVHPDAAPNRYTLEAYPLA
                     GLLPDAPVRFEAGGAELLRAVPGQPAPQPIYLVDLHYADGRVARAQVEAPNWLAPGQR
                     ALSPTGWLRVTDAAGRPQHDAACLTEYQAVYADTMACVRAQPWKREPYFPRLAVRADI
                     PALEFAPGFDEALVSTCEALHEDLYFSLLEHFQQLSGRPPGDRGLQPGQIVPDIRPLM
                     PDDGQARLRVEVREVAAAPAMAPQPAQGSLDEVDAPMPPARIAGWLAGLGGEPFQAVS
                     RQGRPVEGRYLPGPGPAVLISAAQHANETSGVVGALRAAYTLKESGDAHFALIACENP
                     DGYALHQQYCAVHPRHMHHAARYSALGDDVAYREQAPRYEQQARIDALAMSGAQLHVN
                     LHGYPAHEWTRPMSGYLPHLFEAWTIPRGFFLVLRYHPGWQAQARQLIEGVTARLARL
                     PGLLEFNAMSARLYEAHTLQSGFDCINGIAVHSAESRQERAPLAILSEFPDETVYGER
                     FRLAHTVQCTTVLAAVAQYRQQQLLQGETRST"
     gene            12405..12728
                     /locus_tag="VGC69_14460"
     CDS             12405..12728
                     /locus_tag="VGC69_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296527.1"
                     /translation="MKKIPMLIAACLALPCAAALAQAVQEYQFPAAPGSLSPPSTLRI
                     TQDPGPAAPAASGIVRDTPESLGEYQRCRTVSDRAAITNADRQNGVMQCLKALEARRQ
                     GAARQ"
     gene            12725..13318
                     /locus_tag="VGC69_14465"
     CDS             12725..13318
                     /locus_tag="VGC69_14465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0296528.1"
                     /translation="MSADSSHDDAHLIETRVDSELVYDGGFLKVRRDTVRLPNGRDAK
                     REYVVHPGAVVVIAQLDDGRLLVERQHRYPVGRVMTEFPAGKLDPGEDPLACAKRELF
                     EETGYAAGQWAYAGALHLAIAYSTEIIHIFFARGMRAGQRQLDEDEFLDVGAMSLPEM
                     LAASREGRITDAKTLTCLLWLQNVASGAWQLDWQDAA"
BASE COUNT         2091 a         4284 c         4680 g         2344 t
ORIGIN      
        1 agccgtgggt gccaggttca gcgccgacgc catgcgccgc gcccaggcgc tcacctggga
       61 cgccacggca aagattgccg aattggtccg ccccggcatg cgggagtccg aggccatctc
      121 cgccggtaaa gcgctgctcg ccgctcttgg catggagcgt atctggcatc cggttcagat
      181 tcgtttcggg cacaacaccc tgcggacgtt cggcgaaccc agccaggacg atccggtgct
      241 gggtgccgac gacatctact cgaggtggtc caggaccatt ggcgcaccca gcgggtaacg
      301 ggccgggcgc tctatgccta tgccgcaaag caggccgagg ccatgggctg gaagctgaac
      361 ctggacatga agggtcaccg tgtcggcgac tttccgcatg ccatctacaa accggggaag
      421 ctggccgacc tggcggatac gccgagcgag gggctgtgga tcctggagat ccagattgcg
      481 catcccacgc ggccattcgg cgcgttctac gaagatttgc tgcaataacc ccaggtgcct
      541 gtccaggccc tggatatccc gttcggagac ggcggcctca acctgaggcc gatccgcccg
      601 caggtgcttc gtagcgtcgg atgcgggtca gcagcgtgtc tttcagctca cgcgtgactt
      661 tgcgcaggtc gtattcggtc tgcaggttca gccagaagcg gatttccatg ttgaagaaga
      721 tgctcagtcg cagcgctgtg tcggcggtga cggggcgctt gccatgcacg atctcgctga
      781 tgcggctggg cggcacgtcg atgtcggcgg caagccggcg cgcggtgatc tgcatgggtt
      841 tcatgaagtc ttccagcagg atttcgccag ggtgtatgac atccaggggc atgggggaca
      901 tggcggactc ttatcagtgg taatcgacaa tttcgacatc atgggcaccc tcgcccggat
      961 gccaggtaaa gcacacgcgc cattgatcgt tgatgcggat gctgtattgg ccttgccggt
     1021 ctccacgcag cgtttcgagt cgattgccgg gaggcacctg cagcgtgcgc agttcggtgg
     1081 cggcactgat ctgcagcagc ttgcgcaggg caacccgttc gatgtgtcga aaccgtggca
     1141 cgggaaggcc cagatacagc gtttccgtat ccaggcaggc aaatgaaacg atcatagaat
     1201 attccgtaaa acagaatctg taaaacagaa attgatggat gcaggcagag gcaggacaca
     1261 tcatgcccag caagatcact ggcacgtggc cagcaagaga cacgtttgtc actatggcat
     1321 ccaaaaatat cttgctattt agtagtgcac gatttaatat tcaaccatca tgaacgacag
     1381 caccacactt tccacagacg ccgctcccgc cgctgggcag gacgccccgc tgctgctgga
     1441 cgaccagctc tgcttcgcgc tgcattccac ctcgctggcg atgaacaagg tctaccgcaa
     1501 gctgctgggc aagctgggcc tgacctatcc gcagtacctg gtgatgctgg tgctgtggga
     1561 gcgggacggg ctgatcctgt cggatatcgg caagcggctg ttcctggatt cggccacgct
     1621 cacgccgctg ctcaagcggc tggaggcggc cggcctcatc cgccgtaccc gggccgtgcg
     1681 cgacgaacgc caggtcgtca tcgacctgac gcccgagggc cgggcgctgc gcaagcaggc
     1741 ggccgaggtg ccttaccagg tgttctgcgc cacggcctgc acgccccagg cgctgcagca
     1801 gctcaagggc gaactggagc tattgcgcgg tcacctggcc gagtacgaat agggcagggg
     1861 ccatgccggt ggaaattgga tggctattta tatagttcac gatttaatcg ctaaagattt
     1921 tttttgcaac gaatattgag tttcacacaa accgccatgt cggcggcgta ctttgaccca
     1981 agcagtgcct accggtgccc cgcaccgttt tctcaaggag cttcaccatg tctctcgaaa
     2041 aagtcctcta ccgcgcccaa gcccaagcca ccggcggccg tgacggccgc gccgtgtcgt
     2101 ccgacaacgt gctggacgtc aagctgacca ccccgcgcga actgggcggc gccggcgaca
     2161 agggcaccaa ccccgagcag ctcttcgccg cgggctattc ggcctgcttc ctgggcgcca
     2221 tgaagttcgt cgccatgcat gacaagatcg ccatcccgtc ggatgtgtcg gtgcagggca
     2281 atgtgggcat cggccccctg cccaccggct tcggcatcga ggtggaactg aagatttccc
     2341 tgcccggcat ggaccatgcc gcggcgcagg ccctggtcga caaggcgcac atcgtgtgcc
     2401 cgtattcgaa cgcgacccgc ggcaacatcg acgtgacgct cacgctgctg taaggcgtgc
     2461 cgtcgccggg cgaccggcgg ggcggcccgt tccatggcgc tggaaagcgc ctgggcgggc
     2521 cgccttgttt tgcccggggc atgcgagcga tcgttgcgct cccggaacac agttgaccgg
     2581 gccggcgacc aatccgggat tccacacaaa gtagaatccc tgcatttgct tatattccga
     2641 ggtttgcatg caaccggtca tttcggtgca gggcgtgtcc aagacctacg cgtcgggcca
     2701 ccaggccctc aagaacatca atctcgacat ccggcagggt gaaatcttcg ccctgctcgg
     2761 gcccaacggc gcgggcaaga ccacgctgat cggcattatc tgcggcatcg tcaatgccag
     2821 cgccggccgc gtgctggccg acgggcacga catcgtggcc gattaccgcg ccgcgcgcca
     2881 gaccatcggc ctggtgccgc aggagctcac caccgacgcc ttcgagtcgg tgtggagcac
     2941 ggtgtcgttc tcccgcggcc tgttcggcat gccggccaac ccggcgcaca tcgagaaggt
     3001 gctgcgcgac ctttccctgt gggaaaagaa ggacgagcgc atccgcgcgc tgtcgggcgg
     3061 catgaagcgc cgggtgatga tcgccaaggc cctgtcgcac gagccgcgca tcctgttcct
     3121 ggacgagccc accgccgggg tcgacgtgga gctgcgccgc agcatgtggg agatggtgcg
     3181 gcacctgcgc gaaaacggcg tgaccatcat cctcaccacg cactacatcg aggaagccca
     3241 ggaaatggcc gaccgcgtcg gcgtgatccg caagggcgag atcatcctgg tcgaggagaa
     3301 ggcggcgctg atgcgcaaga tgggccgcaa gcagctcacc ctgagcctgg ccgcgccgct
     3361 gccggccatt cccgatgcgc tgcgcggcga gccgctcgag ctttccgccg acggcctcaa
     3421 gctggtctac acctacgacc agcacctcga gggcagcggc agcatcgccg gcctgctggc
     3481 gcgggtgggc caggcgggcc tggtcttcaa cgacctgcag tcatcggaaa gctcgctgga
     3541 agagatcttc gtcagcctgg tgaacgaaca acaaggaaaa acgcaatgaa ctggtacggc
     3601 gtacgcgcga tctatctgtt cgagatggac cgcacctggc gcacggtgat gcagagcgtg
     3661 gcttcgccgg tgatttccac cgccctgtat ttcgtggtgt tcggctcggc catcggctcg
     3721 cgcatggtgc agatcgacgg cgtgagctac ggggccttca tcgtgccggg catggtgatg
     3781 ctgtcgctgc tcacgcagag cacctccaac gcgtccttcg gcatctacat gccgaggttc
     3841 tcgggcacca tctacgaaat cctgtcggcg ccggtgtcgt tcgtcgagat cgtcatggga
     3901 tacgtggggg cggcggccag caagtccatc gtcctgggct gcatcatgct gctcacggcg
     3961 cgggtgttcg tggagttcca catcctgcat ccggtgtgga tggcgggttt cctggtgctg
     4021 acttcgctgg ctttcagcct gttcggcttc attatcggcg tctgggccga cgggttcgag
     4081 aagctgcagg tcatcccgct gatgatcctc acgccgctca ccttcctggg cggcaccttc
     4141 tattcgatca agatgctgcc gccgttctgg cagaaagtga cgctcatcaa cccggtggtg
     4201 tacctgatca acggcttccg ctggagcttc tatggcgtgg ccgacgtgga tgtgggggtg
     4261 agcctgggca tgaccctggt gttcctgctg gtgtgcctgg tcatcatccg gtggattttc
     4321 cacacgggat accggatgaa gcagtaggcc ggcggttggg tttcacggcg ggtttcacgg
     4381 cgtttcatgg cctggcggcc cgctcgccgc ggcgagcggg ccgtgccacg ccgggttatg
     4441 ctggcgggca tgatggatta ccccgcggga ggtcgccatg ccggtctggc tgcccatact
     4501 taaagcttcc cttccttacc tgacgcagat cgtcaccacg gcgatcccgg tgttcacctc
     4561 gcgttcggcc ggcaaggcga gcggcaagcc attgcccgac gaggtggtcg gcaggcagat
     4621 cgccgagctg caggaggccg tcacccagaa tgccgagtcc gtgcacaccc tggccagcaa
     4681 gctgcaggac accttgcagg gcatcgattt cgcggccaat ggcctgcagt cgcagctggc
     4741 cagcctgcgc cggctggcgc gcacggcgct ggtcgtggcg gttgcctccc tgtgcgtggc
     4801 cgcctgggcc ctgacgcgag cctgacccat gcctgaagaa aacgacatcg aacgccgcct
     4861 gatccagctc gaggtcaagg caggttttgc cgacgacctg ctcgaccagc tcaaccacac
     4921 catcttccgc cagcagcagc agatggacct gctgcggcgc gaactggccg aactgcgacg
     4981 ccagatgccg gccagcccgg ggcagccagg cagcctgcgc gacgaactgc caccccacta
     5041 ttgattgata ccgatgcaat ccgctgcgct tggggcaggc aagcccaccc cgacttcctg
     5101 gagccttttc ctgattttta cccgcatcgg cctgaccagc ttcggcggcg ggctgagcgg
     5161 ctggttgatg cgcgagttcg tgcagtcgcg ccagtggatg gacgaagagg agtttctcaa
     5221 cggcctggcg atctcgcagg ccctgcccgg cgtcaatgtg accaatatgt cgatctggat
     5281 cggctaccac ctggccggcg cccggggcgc cgcggccagc ctgtcgggca ttttgctgcc
     5341 gccggccgtc ttcgcggtgc tgctgagcgt gctgttcgcg ctgctgtcgg gctatccgct
     5401 ggtccatgtc tggctggctg gcgcggccgc ggcggccatc ggcctgtcgc tgtccatggc
     5461 catcacggcc gtgcggcggg tgcgccggcg cgtcctgccg ctggtgttca tggtgctggt
     5521 gttcgtgatg gtggccatcc tgcgcctgtc gctggtatgg gtggtgctgg gcctgggccc
     5581 gatcagcgtc gcgctggaat acctgcgctt gcggcgggca gggaagcagg catgatgtcg
     5641 gcattgtggg gtttgttctc gacgttcgtg cccatctcgc tggtgaccgt gggcggcggc
     5701 caggccacgg tcgcggagat ccagcgccag gtggtcgaca cgcatcactg gctgacgcag
     5761 gcgcagtttc tcaatgcctt tgccatcacg cgcatggcgc ccgggccggg ctcgctgctg
     5821 gtgaccctga tcggctggca ggtggccggc ttctgggggg cggtggtggc cacggcggcc
     5881 atgttcggcc ccacggccat cctgctgttc accatcgcct atttctggcg caggcagcgg
     5941 ggcgcgaact ggcagctggc gctggagagc ggcctgcggc ccattgcggc gggcatgatg
     6001 gccgccgcgg tgtacgtgct gatgagcacg ctcgaaggcg gctggatggc gcaggcgatc
     6061 gcgctgatct cgaccgggtt ggtgatgttc acgcgcgtca atgccttgct gctggtgctg
     6121 ggcggcggcg tggcgctgat tctgtgccag atgtcgggcc tggcttgaaa agcttgatgg
     6181 gccggacaag ttccggacat gacgaggggt actgatttgg attggaacgc acagttgagc
     6241 gccagcctgg tctggctggc cgagaccttc tgcatcgtga tcgtggtcgc cgcgatcgcg
     6301 gcggcgatct tcgcgcgcag cacgaagtgg gggcggcagt tctggcgcct ggcctggccg
     6361 tatttcacgc ccaggcgcag ctggaggccg ctggcgatgg tctgcctgct ggtgttcttc
     6421 tcgctcttcg gcgtgcgcat gcaggtgctg ttctcgttct ggtacaacgg cttctacagc
     6481 gccctgcagg ccctggacca aaacgcgttc tggcgcttca tggggctgtt cgcggtgctg
     6541 gcggcggtgc acgtggtgcg tgccttgacg gcctacatca tcggtcaggc cttcgacatc
     6601 cgctggaaga tatggcttaa cgaccggctc acgcaagact ggctgggcaa gcgggcctat
     6661 taccgcgggc agttcgtcga tcatgcggtc gacaaccccg accagcgcat ccagcaggac
     6721 gtcaccctgt tcatcgaggg ctcgcgcacc ctggccatgg gcgtgatcag ctcggcagtg
     6781 tcgctggtgg ccttcacggc cattctctgg agcctgtcgg cgccgctcac gctcatgggg
     6841 gtggagatcc cgcgcgccat ggtgttcctg gtctacgtgt acgtggtggt ggccacctgg
     6901 ctggcgtttc gcatcggccg cccgctgatc cggttgagct tcctggccga gcagctcacg
     6961 gccaatttcc gctatgcctt gatgcgcttg cgcgagaacg ccgagaacgt ggccttctac
     7021 cagggcgagg acgtggagcg cggcacgctg ttcacgcgct tcaaggccta catcgccaat
     7081 caatgggcgt tgatcttccg gaccctgcga ttcgatggct tcaacctggt gatcagccaa
     7141 gtggcggtgg tgtttccctt cctgctgcag gcgccgcgtt ttttcagcgg cgcactcaag
     7201 ctgggcgacg tgatgcagac ctcgcaggcc ttcggccagg tgcaggacgc gctgtcgttc
     7261 ttccgcacgt cctacgacag ctttgcccag taccgcgcca ccctggatcg cctggacggc
     7321 ttcctcgacg ccaattcggc cgcgcgggaa ttgcccgccg tgcaatcggc cgagcaggcc
     7381 gccggcctgc ggctcgaggg cttgtccgtg acgcggcccg acggccaggt gctgttgcgc
     7441 gatctcaacc tgcagttgca gccggggcag tcggtgctga tcaagggacc ctcgggcagc
     7501 ggcaagacca cgctgctgcg cgccgtggcc ggcctgtggc cttatgcgca gggcaaggtg
     7561 gcgcggccgc tgggtgcgca gtcgctattc ctgtcgcagc gtccctacct gccgctgggc
     7621 gacctgcgcg gcgccgtggc ctatccgagc caggccgatc cggccgacga cgcccgcctg
     7681 caggcggccc tgcgcgcggt aaacctcggg cacctgaccg gcaagctggg cgaggtggcc
     7741 gagtggtcgc gcatcctgtc catcggcgag cagcagcgcg tggcgtttgc ccgcgtgctg
     7801 gtcaaccagc ccaaggtgat cttcctggac gaggccacgt ccgccaccga cgagggactg
     7861 gagcacgcgc tctatacgca gttgcgcgcc agcctgcccg agagcgtgct ggtgagcgtg
     7921 gggcaccgcg gcacgctggc ggccttccac agccaccgcc tggagctcga cggcgtgggc
     7981 ggctggcagg tcgcggcaat ttagggcctc gcgtcgacct gatcgctggg cgtggccgcc
     8041 cccccggcgc gttgtcgttt tagcgtcagg cgtaccgatg cagcgccggc gcgggctccg
     8101 gatagggggc gccgccgcgg atgaaggcga tccagtcgcg gcgcatggca tcgctcaggc
     8161 cggcgccgtg cgccgggtcc atgccgtgca gcatgggggc gccggcgaag gtctcgagcg
     8221 tgccgaacac gaagggcagc tcgatgcagt ggcaggcgcc atagcggtcc gtggggccga
     8281 gatccatgcg gtacagccag gcttgccggc cgtgttcctg ggcggactgc gcccactggt
     8341 gcgcgggcgt gccgaagatg cgcatgccca gcgcgtggct ggcctcgtcg gtgccgccgc
     8401 cggggaaggc cgccatttca tcgtgcgtgt agccgatcag cacgtcggcc cgtccggacg
     8461 cctcgggcag gcgcgcgtcg acgtcgagcg gcagcacatc gccgtcggcc acggggcaga
     8521 acaggctgcg cgcgccgcct tcgtccttga gggcctgcag cacggcgggt gcttgctggg
     8581 cctgcagcag ggcctggcag ggcagggctt gcaggtcgtc caggtcgcgc gcgccgaccg
     8641 cgtccatgaa aacctcggtc agcgcgagcg cctgcgcggc gctgcggcag cctcggccga
     8701 tggaggcgct ttgcaggatg gcgcgcgtga actggggttc gcgggtcagc agcagggcgg
     8761 tattgaagcc gcccgccgac tggcccatga ccgttatgcg ttccgggtcg ccgcccagcg
     8821 cggcgatgtg ccggcgcacc cattcgatgg ccagctccat gtcgagcagg cccaggttgg
     8881 cggctacgcc gggcaggtac agccagccca gcgcggccag gcggtagttg atgcccacga
     8941 cgacgacatc gccttcgcgg gccagccgcg cgccgtcgta ccagtcgatc gcgccgccgc
     9001 cgctttgcca ggcgccgccg tgcatccaga tcacgaccgg gcggcggttc gcgtcggcgc
     9061 cgggcgtcca gaccgtgagg tgcaggcagt cctcggactg cgaggcctcg aaatcgccca
     9121 tgacctcgcg caggcgcgaa ggcagctggg gcgcgacggg gccggcttgc gtggcatcga
     9181 tcgcgccggc cagcgtcagg gggcggggcg ggcgcaggcg tagttcgccg acgggcgcct
     9241 gggcataggg aagatggcga tagcagcaaa tgccttgctc acgcgtcccg gtcagggtcg
     9301 cgccggccag gctggtggta acggtggcgc tcatgcgagg ctgctcccgg aagcggtttg
     9361 gacaacccgg gatcatgaaa gagatttgcc gcggcggcaa tgcgtatttg gccgcagtgg
     9421 cgggccggcc gcgcaatggc cggcaaggcg gccgtcagtg cacgctgcgc agcaaggcgt
     9481 agatctgatc cttgatgtac agcttttctt tcttgagttt ctcgaggtcg agcagcgatc
     9541 cggtggcgcg gccggtttcg atgttctgga tgagcaggtc caggtcgccg tgcttttgca
     9601 tcatgcgggc aaagtgcggg ttgtcgctct tgagctgcgt tatctggtct tgaaactcag
     9661 gaaacatgga cgcctccggg gacgaggggc tgcggggagc gcctgtgcgc gaggcacggg
     9721 cgggggggaa cgcattgcct ggggtcttgg ccagtatata ggcggatcag cccggtaaaa
     9781 cggtgccgga cggcagtttc agcgaccagg actggtcatc gaaccggcat tgccagccgt
     9841 ggtgttcggc caggcattgc acctcgtcca tggcgatgcg gccgggcgtg gccggcggca
     9901 cggcaaagcc gcggggggtg gatgggcgag cgaccagatg ccgctcgccg cccatttcga
     9961 tgtgcagcac atcgtcgtgg ggcaagtggt cgagcaggct caggaaccag gccagcagca
    10021 cgtagcgcga accgaattcg ggcaggtcca ggacagggca ggattcaggc aggtcgatct
    10081 gcctggccgc gcgcagcagg tgcccgaaca ggagcttgcg gcaaagttcg agcaggtggt
    10141 ccaggggcag cgtggcgccg gtgccttcca gccagtcgcc caggcgcctc acctcggcgg
    10201 tgctctccag cagcatgctt tccagctcgt tgatcaggcc ttcctggtgg ctgtcggtgc
    10261 cgtgcatgcc atgccgcagc atggccacgg ccaggccggc attcgaaacg ggcgcgatga
    10321 cctcgtgccg cagtaccgga aacacatggg ccagcacttt aaactgcacg ccggccgcaa
    10381 gcgtttcgga gatcggttgc gcaagggatg aggtcatttc gccaaggtat cgcacttgtc
    10441 tgtgacctcg cgttgcattc agtacgccag gcgtaaccag gagcaagcaa gttctccggc
    10501 ctttcaggga gggttcaagt gctgcgcgtt tcgccctgta gcaattgttg ctgccggtat
    10561 tgggcgacgg cggccagcac ggttgtgcac tgcaccgtgt gggcgaggcg gaagcgctcg
    10621 ccgtagaccg tctcgtcggg gaattcgctc aggatggcca gcggcgcgcg ctcctgccgg
    10681 ctttccgcgc tgtgcaccgc aatgccgttg atgcagtcga agccggactg cagggtgtgg
    10741 gcctcgtata gccgggccga catggcattg aattccagca ggcccggcag gcgcgccagg
    10801 cgggccgtca cgccctcgat cagttgccgc gcctgcgcct gccagccggg gtggtagcgc
    10861 agcaccagga aaaagccgcg cgggatggtc caggcctcga agagatgcgg gaggtagccc
    10921 gacatggggc gcgtccattc gtgggcgggg tagccgtgca ggttgacgtg cagctgcgcg
    10981 ccgctcatgg ccagcgcgtc gatgcgggcc tgctgctcgt agcgcggggc ctgttcgcgg
    11041 taggccacgt cgtcgcccag ggcgctgtag cgggcggcat ggtgcatgtg gcgcgggtgc
    11101 acggcgcaat attgctggtg cagcgcgtag ccgtcgggat tctcgcaggc gatgagggcg
    11161 aagtgcgcat cgcccgattc cttgagcgtg taggcggcgc gcagcgcgcc caccacgccc
    11221 gaggtctcgt tggcgtgctg ggcggccgag atcagcacgg ccggccccgg gccgggaagg
    11281 tagcggccct ctaccgggcg gccctggcgc gagacggcct gaaagggctc gccgcccagg
    11341 ccggccagcc agccggcgat gcgggccggc ggcatgggcg catcgacttc gtccaggctg
    11401 ccctgggccg gctgcggcgc catggcgggg gcggccgcga cttcccgcac ctcgacgcgc
    11461 aggcgggcct ggccgtcgtc cggcatcagg gggcggatgt ccggcacgat ctggccgggc
    11521 tgcaggccgc ggtcgcccgg cggtcggccc gacaattgct ggaaatgctc cagcagcgag
    11581 aagtacaggt cttcgtgcag ggcctcgcag gtgctgacca gggcttcgtc gaagcccggg
    11641 gcgaattcga gggcggggat gtccgcgcgc actgccagcc gggggaagta gggttcccgc
    11701 ttccagggct gtgcgcgcac gcaggccatc gtgtcggcgt agacggcctg gtattcggtc
    11761 aggcaggcgg cgtcgtgctg cggcctgccg gcagcgtcgg tcacgcgcag ccagccggtg
    11821 ggcgagaggg cgcgctggcc gggcgccagc cagttgggcg cttcgacctg ggcgcgggcc
    11881 acccggccgt cggcatagtg caggtcgacc aggtagatcg gttgcggggc gggctgtccc
    11941 ggcacggcgc gcagcagttc ggcgccgccc gcttcgaagc ggactggcgc atcgggcagc
    12001 aggccggcca gcgggtaggc ctcgagggtg tagcggttgg gcgcggcgtc cgggtgcacg
    12061 gggtagcgca ccacggccag gcgcagccct tgcaggtcgg cttcttcgag aaagtagtgc
    12121 agcagcggct tgtaggcgct gcgcaggcgg gcaatgacgc cggcctgcgc caggcgggct
    12181 tcggccgccc ggcgcgcctc ggcgctttcg aacagccagc cttcgaggcg gctgccgcga
    12241 tgaccgggac gggtgtattc ggccagccac gcggccaagg tgcgggggtg gcaagtatcg
    12301 agcagaatca tggcatgcct tgtaacacgg cggtccgggc aggggtgccc ggtttgggat
    12361 aaggcgctac catgccattt tcacgccgta ttgccgaggc atccatgaaa aaaatcccca
    12421 tgttgatcgc tgcctgcctg gcgctgccgt gcgccgcggc cctggcgcag gccgtgcagg
    12481 aataccagtt tcccgccgcc ccgggcagcc tttcgccgcc gtccacgctg cgcatcaccc
    12541 aggacccggg cccggctgcg cccgccgcgt ccggcatcgt gcgcgacacc cccgaaagcc
    12601 tgggcgagta ccagcgctgc cgcacggtgt ccgaccgcgc cgccatcacc aatgccgacc
    12661 ggcagaacgg cgtcatgcaa tgcctgaagg cactcgaggc gcgccgccag ggagccgcac
    12721 ggcaatgagc gcagattcct cgcatgacga tgcccacctg atcgaaaccc gggtcgacag
    12781 cgaactggtc tacgacggcg gctttctcaa ggtgcggcgc gacaccgtgc gcctgcccaa
    12841 cggccgcgac gccaagcgcg aatacgtggt gcatcccggc gccgtggtgg tgatcgcgca
    12901 gctcgacgac ggccgcctgc tggtcgagcg gcagcaccgc tacccggtgg gcagggtgat
    12961 gacggagttc ccggccggca agctcgatcc cggcgaagac ccgctggcct gcgccaagcg
    13021 cgagctcttc gaggaaaccg gctatgccgc cgggcagtgg gcctatgccg gcgccctgca
    13081 cctggccatt gcctactcca ccgaaatcat ccacatcttt ttcgcgcgcg gcatgcgggc
    13141 ggggcagcgc cagctcgacg aggacgagtt cctcgacgtg ggcgccatga gcctgcccga
    13201 gatgctggcc gccagccgcg agggccgcat caccgacgcc aagacgctca cctgcctgct
    13261 gtggctgcag aacgtggcca gcggcgcctg gcaattggac tggcaggacg cggcgtgacg
    13321 caggcggcgc acacggcgac gctgcagcct tcgggctggc ggttcgaatg ccgtgggcag
    13381 gccaccgtgc tgcgtgaag
//
LOCUS       DASWPO010000275         7914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2655615, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000275 DASWPO010000000
VERSION     DASWPO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655615"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="dinB"
                     /locus_tag="VGC69_14470"
     CDS             complement(<1..1002)
                     /gene="dinB"
                     /locus_tag="VGC69_14470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY0296529.1"
                     /translation="MTQIERKIVHVDMDAFYASVEQRDRPELRGQPVVVAWTGPRSVV
                     CAASYEAREFGVHSAMSAARAQRLCPQAVYVPPDFERYRAASRQVRAIFARHTDLIEP
                     LSLDEAYLDVTANKQGLASATAVAQAIRAEIHAETGLTASAGVAPNKFLAKIASDWNK
                     PDGLFVIKPARVQAFLQDLPVRKVPGVGKVTQARLDELGIHTVGELGRHAAAELELHF
                     GRYGYRLYELARGIDERPVEPGQAVQQVSAETTFEIDLRLDQLDEALARLATKVWEQA
                     RKKGVAGRTAVLKLKTDRFRILTRSHTQLHPVASPDELAALALGLRERVTLPAYTRYR
                     "
     gene            complement(1095..2288)
                     /locus_tag="VGC69_14475"
     CDS             complement(1095..2288)
                     /locus_tag="VGC69_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0296530.1"
                     /translation="MAAKSNGHAGRQGHQAYETVALVLQGGGALGAYQAGVVEGLGEA
                     GIEPNWVAGISIGALNAAIIAGNPPRRRVERLREFWQTIARQPLLPTDIVVPTDAAQA
                     WPESLVQAFNGMAAWRALTEGQNGFFVPRPAPWLFGARPTPTTASYYDTSPLRHTLER
                     FADFDRINDTRQMRVSVGAVNVRNGNFEYFDNTEQTLRPEHFIASGALPPGFPAVEID
                     GEYYWDGGIVSNTPLYQVLMHEPRKDSLVFQVDLWSAEGDPPTDLTEVATRQKDIQYS
                     SRTRMVTTSMERMQVYRRLLREVMELVPPAKRGNAWYRRAAEMACDRRFNIIHLIYKD
                     KPFDGQAKDFQFGMVSLLDHWNTGLQDMRRTLQHPDWLDMPSGEHSFVTHDVHRKQGH
                     DEVAG"
     gene            complement(2300..3082)
                     /locus_tag="VGC69_14480"
     CDS             complement(2300..3082)
                     /locus_tag="VGC69_14480"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY0296531.1"
                     /translation="MQLEGKVAIITGAASGIGKKIAETYAAQGAAVAIADINLNAAQQ
                     AADEIIAAGGKAMGVAMDVTNEQAVNDGTRQVVDKFGHLDILISNAGIQIVNPIENYS
                     YADWKKMMAIHVDGAFLTTKAALQYMYQDDRGGTVIYMGSVHSHEASPLKSAYVSAKH
                     ALLGLARVLAKEGAKHNVRSHVICPGFVRTPLVDKQIPEQAKELGISEDDVIKKVMLG
                     GTVDGVFTTVDDVAQTALFLAAFPTAALTGQSFIVSHGWFMQ"
     gene            complement(3108..3851)
                     /locus_tag="VGC69_14485"
     CDS             complement(3108..3851)
                     /locus_tag="VGC69_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HEY0296532.1"
                     /translation="MDIKTIRTQAFAMPFSSPAFPVGPYRFIRREFFIITYRTDAARL
                     RQAVPEPLMTPDDPLVNFEFIRMPDSTGFGDYTESGQVIPVTYQGVAGNYTHAMYLDD
                     HPPIAGGRELWGFPKKLASPRLRVASDTLLGELDYGPIRVATATMGYKHHVLDAAASG
                     HALADTPNFLLKIIPHVDGTARICELVRYYMQDVQVHGAWSGPAALALFPHALAPVAD
                     LPVLEIVGARHIVADLTLGLGEVIFDYLA"
     gene            4128..4538
                     /locus_tag="VGC69_14490"
     CDS             4128..4538
                     /locus_tag="VGC69_14490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0296533.1"
                     /translation="MSPPRDYFGRHIPFMNLLGLEAVSLDENQAVTRLPWRADLTNSH
                     GNVHGGTLMSVLDFTLSAACRALDPGLGVITIDMNTHFMEPGRGDLTITARALRRGAT
                     THFCEGEIRDAQGVLVAKAIATFKTVRQSPGGDS"
     gene            complement(4558..5013)
                     /locus_tag="VGC69_14495"
     CDS             complement(4558..5013)
                     /locus_tag="VGC69_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296534.1"
                     /translation="MIKQAKRAGLVLAATALSGCSIGIGTGGSLSGSFEAPVGYRQAY
                     QTALAQAQLCLVGDGAYRVEGRVDETTHAGRLSVVPKLVDGREMARVEVSAAGDARAR
                     VRVEMWGESLWNANAMRAMHDAVYFSAPSCAVYIPPQSGESPDAWFSKK"
     gene            5172..7016
                     /locus_tag="VGC69_14500"
     CDS             5172..7016
                     /locus_tag="VGC69_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0296535.1"
                     /translation="MTTPLYPLQDFFRNPERGYFRLSDDGATLGFMQPVSIDGAPARM
                     NIHVQSLQAGLPAGEPRRLTGETARDISNFFWKGSGTVLYQKDFGGDENFHVLAVDAA
                     SGAVTDLTPYDAVRAHIEDDLPDDPGHVLISHNQRDPQVFDVYRVDVRTGAAQVVAQN
                     PGNVVGWQTDHAGRVRAALTSDGLDTTLLYRDDEAQDFRPLITTDYRTSVSPAFFTFD
                     DRKFYALSNRGRDRLALVVIDPATPDAEERVFEPDAVDLDAAGYSRKRKVLTLAAYQT
                     DKPRHHFFDAQTRDLYERLQAKLPGYDLALQGSNRDEDTFIVAAYNDRTPGSRYLYSV
                     ATDTLAKLADINPAIPEAGMAPVRPIQYTSRDGLVIHGYLTLPVGREPRNLACIVNPH
                     GGPWARDGWGYNPETQFLANRGFCVLQMNFRGSTGYGREFWEASFGQWGLAMQDDITD
                     GVQWLIDQGIADPKRIGIYGGSYGGYATLAGVAFTPDLYAAAVDYVGVSNLFTFMNTI
                     PPYWKPMLDKMHDMVGDPDHDRDRLAATSPALHADRITTPLFVAQGAQDPRVNKAESD
                     QMVAALKARGVEVEYMVKDNEGHGFHNDENKFEFYEAMERFFTQHLKP"
     gene            7016..7555
                     /locus_tag="VGC69_14505"
     CDS             7016..7555
                     /locus_tag="VGC69_14505"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY0296536.1"
                     /translation="MAALNFLLERMATPLGEMLVVTDEHGLLRALDWHDHEARMHLLM
                     RRQYRGDVLALRETKRASPAGQAMLAYFDGDLAAIEGLQVDTGGTDFQRQVWAALRGI
                     PCGETISYLDLATRLGKPLAVRAVGLANGANPISIVVPCHRVIGSNRSLTGYGGGLHR
                     KRWLLDHEQRVGKSGSLAF"
BASE COUNT         1289 a         2805 c         2543 g         1277 t
ORIGIN      
        1 ccggtagcgc gtataagccg gcagcgtgac gcgctcgcgc aggcccagcg ccaacgccgc
       61 cagctcgtcc ggcgacgcca cgggatgcag ctgcgtatgg ctgcgggtca ggatgcggaa
      121 ccggtcggtc ttgagcttga gcactgccgt gcggccggcc acgcccttct tcctcgcctg
      181 ctcccatacc ttggtcgcca gccgcgccag cgcctcgtcg agctggtcca gccgcaaatc
      241 gatctcgaaa gtcgtctcgg ccgacacctg ttgcacggcc tggcccggct ccaccgggcg
      301 ctcgtcgatg ccgcgcgcca actcgtacag gcgatagccg tagcggccga aatgcagctc
      361 cagttcggcc gccgcatgcc gccccagctc tcccaccgta tggatgccga gctcgtccag
      421 ccgggcctgc gtgaccttgc ccacgccggg caccttgcgc accggcaggt cctgcagaaa
      481 ggcctgcacg cgcgcgggct tgatgacgaa caggccgtcg ggcttgttcc agtcggaggc
      541 gatcttggcc aggaatttgt tgggcgccac gcccgccgac gccgtcagac cggtctcggc
      601 gtggatctcg gcgcggatgg cctgggccac cgcggtcgcc gaggccagcc cctgcttgtt
      661 ggcggtgacg tccaggtagg cctcgtccag cgacaggggt tcgatcaggt cggtatgccg
      721 cgcgaagatg gcccgcacct ggcgcgacgc ggcgcgatag cgctcgaaat cgggcggaac
      781 atacaccgcc tgggggcaga gccgctgcgc gcgcgccgcc gacatggccg aatgcacgcc
      841 gaactcgcgg gcctcgtacg aagccgcgca caccaccgag cgcggccccg tccaggccac
      901 caccacgggc tggccgcgca gctcgggacg atcgcgctgc tcgaccgatg catagaacgc
      961 atccatgtcg acgtgcacga tcttgcgttc gatctgggtc atgccggggc tggcggaacg
     1021 gaaatccgga atgccgccat tatcgcgcgc aggccgcccg gtgcgcacaa gcaggcgggc
     1081 gccccccggc gcccttagcc cgcgacctcg tcgtggccct gcttgcgatg cacgtcgtgc
     1141 gtgacgaagc tgtgctcgcc cgagggcatg tccagccagt ccgggtgctg cagggtgcgc
     1201 cgcatgtcct gcaggccggt gttccagtga tccagcaggc tcaccatgcc gaactggaaa
     1261 tccttggcct ggccgtcgaa gggcttgtcc ttgtagatca ggtggatgat gttgaagcgc
     1321 cggtcgcacg ccatctcggc ggcgcggcga taccaggcgt tgccgcgctt ggccgggggc
     1381 accagctcca tgacctcgcg cagcaggcgg cggtagacct gcatgcgctc catggacgtc
     1441 gtcaccatgc gggtgcggct ggagtactgg atgtccttct ggcgcgtcgc cacctccgtc
     1501 aggtcggtcg gcggatcgcc ctcggcgctc cagaggtcca cctggaacac cagcgagtcc
     1561 ttgcgcggct cgtgcatcag cacctggtag agcggcgtgt tcgagacgat gccgccgtcc
     1621 cagtagtatt cgccgtcgat ctccaccgcc ggaaagcccg gcggcagcgc gccggaagca
     1681 atgaaatgct cgggacgcaa ggtctgctcg gtattgtcga agtattcgaa attgccgttg
     1741 cgcacattga ccgcgcccac cgacacccgc atctggcgcg tatcgttgat gcgatcgaag
     1801 tcggcgaagc gctccagcgt gtggcgcagc ggcgaagtgt cgtagtagct ggccgtggtg
     1861 ggcgtgggcc gggcgccgaa cagccagggg gccggacgcg gcacgaagaa gccgttctgg
     1921 ccctcggtca gcgcgcgcca ggccgccatg ccgttgaagg cctgcaccag cgactcgggc
     1981 caggcctgcg cggcgtcggt gggcaccacg atgtcggtag gcaagagggg ctggcgcgcg
     2041 atggtctgcc agaactcgcg cagccgctcg acgcggcgcc gcggcgggtt gcccgcgatg
     2101 atggccgcgt tgagcgcgcc gatggaaatg cccgccaccc agttgggctc gatgccggcc
     2161 tcgcccaggc cttccaccac gcccgcctgg taggcgccca gcgcgccgcc gccctgcagc
     2221 accagggcga cggtctcgta ggcctggtgg ccctgccggc cggcgtggcc gttggatttc
     2281 gccgccatgc cggctcccct tattgcatga accaaccgtg actgacgatg aacgactgtc
     2341 cggtcagcgc cgccgtcggg aaggccgcca ggaacagcgc ggtctgggcc acgtcgtcga
     2401 cggtggtgaa cacaccgtcg accgtgccgc ccagcatcac cttcttgatc acgtcgtcct
     2461 cgctgatgcc gagctccttg gcctgctcgg ggatctgctt gtccaccagc ggggtgcgca
     2521 cgaagccggg gcagatcacg tgcgaacgca cgttgtgctt ggcgccttcc ttggccagca
     2581 cccgcgccag gcccagcagc gcgtgcttgg ccgacacata ggccgacttc agcggcgagg
     2641 cctcgtgcga gtgcaccgaa cccatgtaga tcaccgtgcc gccgcggtcg tcctggtaca
     2701 tgtactgcag cgccgccttg gtggtgagga aggcgccgtc gacgtggatg gccatcatct
     2761 tcttccaatc ggcgtagctg tagttctcga tggggttgac gatctggatg ccggcattgg
     2821 agatcaggat gtcgagatgg ccgaacttgt cgaccacctg gcgggtgcca tcgttgaccg
     2881 cctgttcgtt ggtgacgtcc atggccacgc ccatcgcctt gccgccggcc gcgatgattt
     2941 cgtcggcggc ctgctgcgcg gcattcaggt tgatatcggc gatggccacc gcggcgccct
     3001 gcgcggcgta ggtttccgcg atcttcttgc cgatgccgct ggctgcgccc gtgatgatgg
     3061 cgacttttcc ttcgagttgc atgattatct tcctctcgtt tcgattgtca ggcgaggtag
     3121 tcgaaaatga cctcgcccag gcccagcgtc aggtccgcca cgatgtggcg cgcgccgacg
     3181 atttccagca ccggcagatc ggccaccggc gccagggcat ggggaaacag cgccagcgcg
     3241 gccgggccgc tccacgcccc atgaacttgt acatcctgca tgtagtagcg caccagctcg
     3301 cagatgcggg cggtgccgtc cacatgggga atgatcttga gcaggaaatt cggcgtgtcg
     3361 gccagcgcgt ggccgctggc cgccgcatcc agcacatggt gcttgtagcc catggtggcc
     3421 gtcgccacgc ggatggggcc gtaatccagc tcgcccaaca gcgtgtcgct ggccacccgc
     3481 agccgcgggc tcgccagctt cttgggaaag ccccacagct cgcgcccgcc ggcgatgggc
     3541 gggtggtcgt ccaggtacat cgcgtgcgtg tagttgcccg ccacgccctg atacgtcacg
     3601 gggatgacct ggccgctttc cgtgtagtcg ccaaagccgg tcgagtccgg catgcggatg
     3661 aattcgaaat tcaccagcgg atcgtccggc gtcatcaacg gctcgggcac ggcctggcgc
     3721 agcctggccg cgtcggtacg gtaagtgatg atgaaaaatt cgcggcggat gaaccggtag
     3781 ggcccgacgg gaaaagccgg gctggagaac ggcattgcga acgcctgtgt gcgtatcgtc
     3841 ttgatatcca tgggctgtgc aactccggga tgcggctgag aaccgtgcgc cgccaacggt
     3901 attccaaact ccggcacaac agtatgtctg gaaacatttc gggacacact tgccagcttg
     3961 ccgaagtgct atgacagcgc gctgaaatgc gctgcgccgt gccgctccgg ccgaggacca
     4021 acagctaggc gtcaggcgtc tcgcgcggat tcaaaacggc atatcatgac ccgctatcca
     4081 gccagccctc cccgcccctc acgaaccgat atcccgaacc gagacccatg agccccccgc
     4141 gcgattattt cggccgccac atccccttca tgaacctgct gggactggaa gccgtctcgc
     4201 tcgacgagaa ccaagccgtc acccgcctgc cctggcgcgc cgacctcacc aacagccacg
     4261 gcaacgtcca cggcggcacc ctgatgagcg tgctggactt caccctgagc gcggcctgcc
     4321 gcgccctcga ccccggcctg ggggtgatca ccatcgacat gaacacgcac ttcatggaac
     4381 ccggccgcgg cgacctcacc attaccgcgc gcgccctgcg ccgcggcgcc acgacgcact
     4441 tctgcgaagg cgaaatacgc gatgcccagg gcgtgctcgt ggccaaggcc atcgccacct
     4501 tcaagacagt caggcaatct ccgggcggcg actcgtagca gcgcggcgcg cagcggccta
     4561 tttcttgctg aaccaggcgt cgggcgactc gccgctctgg ggcgggatat aaacggcgca
     4621 cgacggcgcg ctgaaataca ccgcgtcgtg catggcccgc atggcgttgg cattccacag
     4681 gctctcgccc cacatctcca cccgcacgcg ggcgcgggca tcgcccgccg cgctcacctc
     4741 gacgcgggcc atttcccggc cgtcgaccag cttgggcacc acgctcaggc ggccggcatg
     4801 cgtggtctcg tccacgcgcc cctcgacacg gtaggcgccg tcgcccacca ggcaaagctg
     4861 ggcctgcgcg agcgccgtct gataggcctg ccgataaccc accggcgcct cgaaactacc
     4921 cgatagtgat ccgcccgtgc caatgccgat cgaacagccc gacagggcgg tcgcggccaa
     4981 tacgaggccg gcgcgtttcg cttgcttgat catgatgtct cccaatcgca gagtccccta
     5041 ttatcccgca tgccgggaag ttcgacatca accgcttccg ggcgttacgc gcgttcgaca
     5101 ccgcggcaag gcctgtggca tgatcgtctg tcccgaccgg cctgtttcga ccggacgcca
     5161 cggagaccgc attgaccacg cccctctatc cgctgcagga cttctttcgc aaccccgaac
     5221 gcggctattt ccggctgtcg gacgacggcg cgaccctggg cttcatgcag cccgtctcga
     5281 tcgacggcgc gccggctcgc atgaacatcc acgtgcagtc cctgcaagcc ggcctgccgg
     5341 ccggcgagcc ccgccgcctc accggcgaga cggcccgcga catcagcaat ttcttctgga
     5401 agggctcggg caccgtgctc taccagaaag acttcggcgg cgatgagaac ttccatgtgc
     5461 tggccgtcga cgccgccagc ggcgccgtca ccgacctcac gccctacgac gcggtgcgcg
     5521 cccacatcga ggacgacctg cccgacgacc ccgggcacgt gctcatcagc cacaaccagc
     5581 gcgacccgca agtcttcgac gtctaccgcg tcgacgtgcg cacgggcgcg gcgcaggtcg
     5641 tggcgcagaa ccccggcaac gtcgtcggct ggcagaccga ccatgccggc cgcgtgcgcg
     5701 cggcgctgac cagcgacggc ctggacacca ccctgctcta ccgcgacgac gaagcgcagg
     5761 acttccgccc gctcatcacc accgactacc gcaccagcgt cagcccggcc ttcttcacct
     5821 tcgacgaccg caagttctac gccctgagca accgcggccg cgaccgcctg gccctggtcg
     5881 tcatcgatcc ggccacgccc gatgccgaag agcgggtctt cgaacccgac gccgtcgacc
     5941 tcgacgccgc cggctattcg cgcaagcgca aggtgctgac cctggcggcc taccagaccg
     6001 acaagccgcg ccaccacttc ttcgacgcgc agacccgcga cctgtacgaa cgcctgcagg
     6061 cgaaactgcc cggctacgac ctggccctgc agggcagcaa ccgcgacgag gacaccttca
     6121 tcgtggccgc ctacaacgac cgcacgcccg gctcgcgcta cctgtacagc gtggccaccg
     6181 acacgctcgc caagctggcc gacatcaacc ccgccatccc cgaggccggc atggccccgg
     6241 tgcgccccat ccagtacacc agccgcgacg gccttgtcat acacggctac ctgaccctgc
     6301 ccgtgggccg cgaaccgcgc aacctggcct gcatcgtcaa cccgcacggc ggcccctggg
     6361 cgcgcgacgg ctggggctac aaccccgaga cgcagttcct ggccaaccgc ggcttctgcg
     6421 tgctgcagat gaacttccgc ggctccaccg gctacggccg cgagttctgg gaagccagct
     6481 tcggccaatg gggcctggcc atgcaggacg acatcaccga cggcgtgcaa tggctcatcg
     6541 accagggcat cgccgacccc aagcgcatcg gcatctacgg cggcagctac ggcggctacg
     6601 ccaccctggc cggcgtggcc ttcacgcccg acctgtacgc ggcggcggtg gactatgtcg
     6661 gggtgtcgaa cctgttcacg ttcatgaaca ccatcccgcc ctactggaag cccatgctcg
     6721 acaagatgca cgacatggtg ggcgacccgg accacgaccg cgaccgcctg gccgccacct
     6781 ctcccgccct gcacgccgac cgcatcacca cgccgctctt cgtcgcccag ggcgcgcagg
     6841 atccgcgcgt gaacaaggcc gaaagcgacc agatggtggc ggcgctcaag gcccgcggcg
     6901 tcgaggtgga atacatggtc aaggacaacg aaggccacgg ttttcacaac gacgagaaca
     6961 agttcgagtt ctatgaagcc atggaacgct tcttcacgca gcacttgaaa ccctgatggc
     7021 cgccctgaat ttcctgctgg agcgcatggc gacgccgctg ggcgagatgc tggtcgtcac
     7081 ggacgaacac ggcctgctgc gcgcgctcga ctggcacgac cacgaagcgc gcatgcacct
     7141 gctgatgcgc aggcaatatc gcggggacgt gttggcgctg cgggaaacga agcgggcctc
     7201 gcccgccggg caggccatgc tggcgtattt cgacggcgac cttgccgcca tcgaggggct
     7261 gcaggtcgat accggcggca cggatttcca gcgccaggtc tgggcggcgc tgcgcggcat
     7321 tccctgcggc gaaacgatca gctacctgga cctcgccacc cgcctgggca agcccctggc
     7381 cgtgcgcgcg gtaggcctgg ccaacggcgc caaccccatc agcatcgtgg tgccctgcca
     7441 tcgggtgatc ggcagcaatc gctcgctgac cggctacggc gggggattgc accgcaagcg
     7501 atggctgctt gatcatgaac agcgggttgg caagtctggg agtttggcct tttaggctag
     7561 taggtacgtt gtattgcgtt gccgcatggg catgggcatg ggcaatcgca ttctattgac
     7621 gtcagtcggg gcggggtgag gcggtgggcg cgccgctggg ctcggtcggg cgccctgcgc
     7681 gcccgacacc gcatcggctc gctcgtccgc gcctccgcgc tcccttcgct tccgccttgc
     7741 tcggcccaga ggcgcgccca ccgcctcacc ccgccccgac tgactgcagt agtttgtgtt
     7801 cccagcattg ctggggacgt gttgggtgct tggcgcttcg tgggccaagg ctgggggtgg
     7861 gaggaaaatc ttggttcgct cgccaaaccg ggctgccctg cgcagcccgg tttg
//
LOCUS       DASWPO010000276        10926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3010430, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000276 DASWPO010000000
VERSION     DASWPO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010430"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VGC69_14510"
     CDS             complement(<1..430)
                     /locus_tag="VGC69_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEY0296537.1"
                     /translation="MAKAIVILASFALAGCTTISANNTASATQQRNEINAQANATLTK
                     LYQAAPQTKSLVGQSKGILIFPSIVGASFIVGAEHGKGVLRQNDQPVAYYSTTSGSVG
                     LQAGAQSRATVLLFMTDEALLNFKKSEGWTAGADAKVAVAN"
     gene            complement(538..1302)
                     /locus_tag="VGC69_14515"
     CDS             complement(538..1302)
                     /locus_tag="VGC69_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HEY0296538.1"
                     /translation="MACAALALCVGLGQARADSVEITAPLNGWRNSSGDEARYTQDVH
                     YPAVSVSTPEGQSASALIAGRIKAAPKAKQHASVGTLVVNGTPLPQRIEEDGTFSRPY
                     AFGAGSNSVEMRAAGGANSRLQFYDAYAGKTHARLRIVLAWDTDGTDLDLHVVSPDGG
                     HTFYGDRVSSNGGALDVDVTTGYGPEIFSSAAPLPGTYLVYVNYYGSGEQESVITTAQ
                     ITVITDEDTPNEKSETIRVPMRKPGELTLVKRFAMP"
     gene            complement(1391..2341)
                     /locus_tag="VGC69_14520"
     CDS             complement(1391..2341)
                     /locus_tag="VGC69_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HEY0296539.1"
                     /translation="MTTARAWSVRRHGRLPGLDRLRWLALCACLGGLTAGPLPTKAAG
                     TALAESQVAEKGGGKEGEKGAGDAEGDGSVARLTGAFGGWRHSGLAREGERYVAAYPR
                     PPVDRGQQRFRTLIEGQLRHIDPASRRPPTLVVNGNATPLYADSQGWFARPWAFGAGS
                     NSIELISPDGRTRQRMQFYEADRSVPQALLRAIVTWDDPHAQVDLHVITPDGLHAFFA
                     NPILADGSGFDVDSVDGAGPGIFSSAAPQHGAWLFFLNYWGNFDENGYNFRAGSHDKD
                     VITATLTLVFNENTPNERRETSVVPLRKIGDLMLVKRAQL"
     gene            complement(2338..4161)
                     /locus_tag="VGC69_14525"
     CDS             complement(2338..4161)
                     /locus_tag="VGC69_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HEY0296540.1"
                     /translation="MPLWARNTVQGRTRSTETARRLARGLFRLLVLGLAAGVGASPSA
                     ASPSGAVHAVPPAAGQGQGRPDSALRFAWLRAGDARLWTLDPIDGQDSPDAGQPLPDT
                     LRTPLGSVWKLFVYAYLIDRQIPAPDYACDGGNRDEVYCCTAGGSIDRDHALVQSCGL
                     FFEPGRLALDPQAWRRYWRALDAPAWLEDLGALGPQRRVAVADLLKALARIPSRARDE
                     TAATLVSVVTVGRGQNTVSRYGSLLRVKTWTMPDPADDKAYVGGAAGWLADGTPVWLG
                     GRGGSRQVLVQAAQPLAPWVAASAVPDDHACVAVDFFSRYSIREVDGPEPDRRPAPAG
                     LLDGAWRVHFQNGNAISVIGHGDLLLDRPADGAPRIVGRFGMNDYVARVVEREGSAVQ
                     PQAARALAVAVRSYLVQNAGRERGCYRIDDSSRTQRVLPRPASRAARAAADFTDGLVL
                     VGTPVRFHLNQAAPGQMSWQAAQADARSGLGFDAILARTWPQADLAYFTSPLAGDCLP
                     LLRAQDWLRLEAPRWEARLAGEAGYELPDQPAVCELNSGRPYADARRNRIYIQRLVSD
                     EDRITLAHEYLHLAFAHHPRGLDEQFIERTARKLIYTGDNQ"
     gene            complement(4178..9115)
                     /locus_tag="VGC69_14530"
     CDS             complement(4178..9115)
                     /locus_tag="VGC69_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEY0296541.1"
                     /translation="MVVMEDAQGGTGRGGADCEPGGRRLAHPLARGLLARGFALLLAA
                     AALTLSPVVWADDATPAETTQGDSQADDGQVSPQDSIDANPTLAASQPSGYGDQVVRG
                     QPFFLLSDASFGSDQVAQVRLEAPGEEFQSELAAYGGADIVVYKVPDPQAFLKAQKNL
                     HRLNIAPNYQGEGLANTLAFLWDRWLGETRHAWQRVFSYETRSRVTQAKPEFKLGEQP
                     NAPTRFEPSNRFAPLRGYPMVSRFRYPIWDAKTIKPPKDVNLAGSSSNFITPSLGNVM
                     IPVGKQAPGLYLVEAIIGNYRAHTLLFVSDTVAIVKSSSSGLLVWTARRDNGKPMAGS
                     VVDWTDGVGVLQKGATGADGTLALGHVSPERSYVLGTDAAGGVFVSENFYYDSEIYNT
                     KIYAVTDRPMYRPGDTVHVKFIGRAFQSANRSTAAVPATLALEVLDPNGAPLATRQVD
                     FAADSGGDTRFSLPDNAPGGGYTLRFNYQGSLYGAAFRVADYVKPHFDVNLTMDKADY
                     ATGEAPKGKIALRYPDGKPVKNGKVSLTLRAQKVAIADGELRYAGLFPVKLAQQALQT
                     DDQGNADIALPVAKDPSRYALTLFAQDGAAYRVRVTREILIARGATTYRLAADKAFSP
                     PREPVMFNLTPAAMSGGASAGHPPATWEWLRLESRDTKRGSLPVAGPDGRLSFPVQFD
                     APGSYMLSVKDAAGNLLAAASHWVAGDGLKAVPGSVEIVFDHPRYRVGDTAEALITFP
                     YPVDDALLTLERDSVEGKALLSTGGDWLDLERVAPTQWKARIKVGPGYAPNMTFSVLY
                     VHDGEYAFQNAGIVVAKPAIDLTVASDKPVYQPGETVTLDLQSAFGGKPIAAHLTVSV
                     VDEMVYVLQPEIAPDIAEFFYHPRRNNVRTSSSLSFITYDLALSAMRGAPGGPQRGQY
                     NERGVKVLERPRRDEVDTAAWQADLVTDAQGRTRMQFRMPDSLARWRITVRAVSTEGD
                     VGQRTDYIRSDKDFYLKWSGPTHFRRGDRPSVDVLAFNQSQTEKKARWVVDGAGLSVD
                     SQVTLKPGANYLQLPRVALGDGMVDTRLEVDGKPVDRLQTPVGLVTPGWLDLRQADVA
                     LDTNAAPLALPADAQDVRVRFVSNAASRFARVMDDLIDYPYGCVEQTSSRLIPLSLAY
                     DTLRAAGKPAGSSTQDLEARLRNQRQRLAMMAGVNGTFGWWGDLTGGNAFLTTYAYYA
                     DWLASHSLGVVLPDANWQHVLEVYRDHAAKEPLLHRALALWLLQQMGQPVGTQVSGVA
                     EQLAKQGQRLVGQGLVSEPQDGDTLEGSLVFMAPDSDTGLEAAILLTAYTAKAGGVRL
                     PEGYAGLAERARRALSGDTSPMIQALQAMTGDASRVSLDAVLAHSSASYSTVDRALTL
                     LWLRKAFNGVGDAPGSLPSPREADWKAQSTPSGMSSWLWTGKNLPASLEVEGGGASAA
                     AVVSYRSGQAQDSGLPLTIERVLYRLDPVAPQGADQGKNPGGGTSNSTDAGPGIGGGG
                     SRDDSMGRASFQAVRVQPGDPLDSSALYVDEVRLAPQGGSILQYGLLDVPLPPGGSVE
                     ATSWGVSIAGLPGNDQGKDAQPFARAATYEMGELSYHQPIPLLAKPVVLRQLVRFAVP
                     GTFVLPPARYFRMYQPAYKAYEGGRSDHMATLAIQ"
     gene            complement(9118..9810)
                     /locus_tag="VGC69_14535"
     CDS             complement(9118..9810)
                     /locus_tag="VGC69_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HEY0296542.1"
                     /translation="MRRREFLRCHAALGAAAAGGLLPGLARALTASGEPDDAQALSPA
                     QSAIFRAWFVRIVDAQLDRGPTPRWTQRDCAGLVRFAAMQALRKHDERWLKANGMLGE
                     SDVSRLPPELALNAGQRTLAQRWTRFDGTVGAYASAIALVQDNSHFVSRDVNQALPGD
                     LLFFDQGDDQHLMVWLDRYIAYHTGTVTPTDNGLRAVAVSDLMQWKDSRWRPLDGNPN
                     FAGIFRLAFLRP"
     gene            complement(9810..>10926)
                     /locus_tag="VGC69_14540"
     CDS             complement(9810..>10926)
                     /locus_tag="VGC69_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HEY0296543.1"
                     /translation="APAVADGHRITISANYLSFGYQAFFSGIDAVRFDFGQAHAAPAQ
                     AGGESAWSTAALIDPARLPQQWNGEALWQALPANAAACATLPVDWNDAAGLLEKLGPD
                     LAGSAKALHDHLEGPAAVCWYAASTLASPVFVARLTKDAMGNAAFSGAMAQLFDRVIG
                     AYEARAGDSPGYRRLPVQACKASEGVSCWMRPVSARAGTASSRAAPYAEQLSTPRYFP
                     VTLALAHGYAVFSPDARLVDDTLAVLDKRYPSEADTLAPQPARATVAVITPSSLAALA
                     EREAGRTLPADQEAIFRNAARQHLLPKLHAFAAYAPVSLSLPDSPLPSAAGWVPVTWR
                     ANAKAGGVPRDDAAMFPPHRSPVDDSGFGDDAAASEN"
BASE COUNT         1834 a         3711 c         3593 g         1788 t
ORIGIN      
        1 tattggccac ggcgaccttc gcatcggcgc cggccgtcca gccctcgctt ttcttgaaat
       61 tcagaagtgc ttcgtcagtc atgaaaagca ggaccgttgc gcgagactgt gcgccggcct
      121 gcaggccgac agacccggag gtggttgaat aataggccac gggctggtca ttttgccgaa
      181 gcacgccttt gccatgctcc gcgccgacga tgaagctcgc accgacaata gagggaaaaa
      241 tcaagatacc cttggattgg cccaccagac tcttcgtttg gggagccgcc tggtaaagct
      301 tcgtcagggt tgcattggcc tgcgcgttga tctcattgcg ctgttgcgtc gcgctcgccg
      361 tattgtttgc cgagatggtc gtacaaccgg cgagcgcgaa gctcgccaga atcacgattg
      421 ccttggccat gctcgcacgg cggaaaaaca ggtgaactcc agccataact ttctccttag
      481 aatcctgcaa ggcaggcaat agcaccaagc tcttcgccgt ctttgagatt gacctcatca
      541 tggcatggcg aatcgtttta cgagcgtgag ttccccaggc ttgcgcatcg ggacgcggat
      601 cgtctcgctt ttttcgttcg gcgtatcttc atcggtaatg acggtgatct gcgccgtcgt
      661 gatcacgctt tcctgctcac cgctgccata gtagttgacg taaacaagat aggtcccagg
      721 cagcggagcg gcactcgaga agatctccgg cccatagccc gtggtgacgt caacatcgag
      781 cgcgccgccg ttggatgaca cccggtcgcc gtagaacgtg tgcccgccat ccggcgacac
      841 cacgtgcaag tccaggtcgg ttccatcggt gtcccacgcc agcacgatac gcagccgcgc
      901 gtgcgtcttg cctgcgtagg cgtcatagaa ttgcaggcgg ctgttcgcac cgcccgccgc
      961 gcgcatctcc acgctgttgc tgcccgcgcc gaatgcatag ggacgagaaa aggtgccgtc
     1021 ttcttcgatg cgctgcggca gcggcgtgcc attcacgacg agcgtgccca ccgaggcatg
     1081 ctgcttcgcc ttgggcgccg ccttgatccg gcccgcgatc aacgcgctgg ccgattgccc
     1141 ttccggggtg ctcacggaca cggccgggta gtgcacgtct tgcgtgtagc gcgcctcgtc
     1201 tcccgacgaa ttgcgccagc cattgagggg cgccgtgatc tccacgctgt ccgcgcgcgc
     1261 ctgccccaag cccacgcaca aagccagcgc ggcgcacgcc atcgaacgcc ggaagaaccg
     1321 gcggcacgat agcgccaggg ttcctcgtgt tttcgcttgg ttcattttaa ggaagcatcc
     1381 gttcggtcga tcataattgc gctcgcttga ccagcatcag atcgccaatc ttgcgcagcg
     1441 ggaccacgct ggtctcacga cgctcgttgg gggtgttttc attgaacacc agggtcagcg
     1501 tcgcggtgat cacgtcttta tcgtgggagc cagcccggaa gttgtagccg ttctcgtcga
     1561 aattacccca atagttcaga aagaacagcc aggccccgtg ctggggagcc gccgacgaaa
     1621 agatgccggg tcccgcgcca tcgacggaat ccacatcgaa accgctgccg tcggccagga
     1681 tggggttggc aaagaacgcg tgcaaaccgt ccggcgtgat gacatgcagg tccacctggg
     1741 catgcggatc gtcccaggtg acaatggcac gcagcaaggc ctggggcacg ctgcggtcgg
     1801 cctcgtagaa ctgcatgcgc tggcgcgttc tgccatcggg ggaaatcagc tcgatgctgt
     1861 tggaacccgc gccgaacgcc cagggccggg cgaaccaacc ctggctgtcg gcgtacaacg
     1921 gggtggcgtt gccattgaca accagggtgg gcggacggcg cgacgcgggg tcgatgtgcc
     1981 gcagctgccc ttcgatcagc gtgcgaaaac gctgttggcc gcggtcgacg ggcggacgcg
     2041 gataggcggc gacgtagcgt tcgccttctc gcgccaagcc actgtgacgc catccgccaa
     2101 aggcccccgt cagccgcgcg accgaaccgt caccctcggc gtcgccagcc cctttttctc
     2161 cctctttgcc gccccccttt tcggcgacct ggctctcggc cagcgccgtg ccggcggctt
     2221 tcgtgggcaa ggggccggcc gtcaagccgc ccaggcaagc gcacagagcc agccagcgca
     2281 ggcggtccag tcctggcagc cgcccgtggc gccgcaccga ccaggcacgc gctgtcgtca
     2341 ttgattgtct cccgtgtaaa tcagcttacg ggcggtccgc tcgatgaatt gctcgtccag
     2401 cccacgcgga tggtgggcaa aggccaggtg cagatactcg tgcgcgagcg taatgcggtc
     2461 ttcatctgac accaggcgct ggatatagat gcgattgcgg cgcgcatcgg cgtaaggccg
     2521 gcccgagttc agctcgcaca cagccggttg gtcgggcagt tcatagccgg cctcgccggc
     2581 cagccgggcc tcccatcggg gcgcctcgag ccgcagccag tcctgcgccc gcaacaacgg
     2641 caggcaatcc cccgccagcg gactggtgaa atacgccaga tcggcctgcg gccaggttcg
     2701 cgccagaatg gcgtcaaagc ccagcccgct ccgggcgtcg gcttgcgcgg cctgccaact
     2761 catttggccg ggcgcggcct gattgaggtg gaaccgcacg ggcgtcccca cgagcaccag
     2821 cccgtcagtg aagtcggccg ccgcgcgggc ggcgcggctg gccgggcgcg gcagcacgcg
     2881 ctgcgtgcgg ctgctgtcgt cgatgcggta gcacccccgc tcgcgcccgg cgttctgcac
     2941 gagatagctg cgcacggcca cggcaagcgc tctggccgct tgcggctgga cggcgctgcc
     3001 ttcacgctcc accacccgcg ccacatagtc attcatgccg aagcggccaa cgatgcgggg
     3061 agcgccgtcg gccggcctgt ccagcagcag gtcgccgtgg ccgatcaccg aaatcgcgtt
     3121 gccgttctgg aaatgcacgc gccaggcgcc atccagcagg ccggccggag ccggcctgcg
     3181 atccggttcc gggccgtcca cctcccggat ggaataacgg gagaaaaaat cgaccgccac
     3241 acaggcatga tcatcgggca ccgcgctggc cgccacccac ggcgccaggg gctgcgccgc
     3301 ctggaccagc acctggcggc tgccgccgcg cccgcccagc cagactggcg tgccgtcggc
     3361 cagccagcct gcagcgccgc ccacataggc cttgtcgtcg gcgggatcgg gcatggtcca
     3421 ggtcttgacg cgcagaaggc tgccgtagcg cgacaccgta ttttgtcccc ggccgacggt
     3481 caccactgat acgagcgtgg cggcggtttc gtcgcgcgcg cgcgatggga tgcgcgccag
     3541 cgccttcagc agatccgcca cggccacgcg ccgttgcggc cccagggcgc ccaggtcttc
     3601 cagccaggcg ggcgcgtcca gcgcgcgcca atagcggcgc caggcctgcg gatccagagc
     3661 cagccgccct ggctcgaaaa acaatccgca ggattgcacc agcgcgtggt cgcggtcgat
     3721 gctgccgccc gccgtgcagc aatacacctc gtcgcggttg ccgccatcgc aggcgtagtc
     3781 gggcgcggga atctgccggt cgatgagata ggcgtagacg aatagtttcc agacgctgcc
     3841 cagcggcgtg cgcagggtat cgggcagcgg ctgtcccgcg tccgggctgt cctgcccatc
     3901 gataggatcc agcgtccata ggcgcgcgtc gcccgcgcgc agccaggcaa agcgcagcgc
     3961 gctgtcgggc cggccctggc cctggccggc cgccggcggc acggcgtgga cggcaccgga
     4021 aggggatgcc gccgacggcg aagcgccgac accggcggcc agccccagga caaggagacg
     4081 gaacaagccg cgggccaggc gccgggccgt ctccgtagat cgcgtccggc cctgcactgt
     4141 gttcctggcc caaagcggca tcatcccggt ctcgctctca ctgtatcgcc agcgtggcca
     4201 tgtggtcgct gcgcccgccc tcgtaagcct tgtaggccgg ctggtacatg cggaaatacc
     4261 gggcgggcgg cagcacgaag gtaccgggca ccgcgaagcg caccaattga cgcaagacga
     4321 ctggcttggc cagcaacggt atgggctggt gatagctgag ctcgcccatc tcgtaggtcg
     4381 ccgcgcgggc gaagggctgc gcgtccttgc cctggtcgtt gccgggcagg cccgcgatgc
     4441 tgacccccca gctcgtggcc tcgaccgaac cgcctggcgg cagcggcacg tcgagcaggc
     4501 cgtactgcag tatggaacca ccttgcggcg cgagacgaac ctcatcaaca tacagcgcgc
     4561 tgctgtcgag ggggtctcca ggttgcacgc ggacggcctg aaagctggct cgacccatgc
     4621 tgtcgtcacg tgatcccccg ccgccgatgc ccgggccggc atccgtgctg ttcgacgtgc
     4681 cgcctccggg gtttttgcct tggtccgcgc cttgcggcgc gacgggatcc aggcggtaca
     4741 gcacacgctc gatcgtgagc ggcaagccgc tgtcctgcgc ctggccgcta cggtaggaca
     4801 ccaccgccgc ggcggacgcc cccccaccct cgacctccag gctggctggc aagttcttgc
     4861 ccgtccatag ccaggaggac atgccggacg gcgtggattg cgccttccag tccgcctccc
     4921 gcgggctggg caggctgcca ggcgcatcgc cgacgccatt gaaggccttg cgcagccaaa
     4981 gcagcgtcag cgcgcgatcc accgtcgaat aggatgcact gctgtgcgcc agcaccgcat
     5041 ccaggcttac gcgggaggcg tcgcccgtca tggcctgcaa ggcctggatc ataggggatg
     5101 tgtcgcccga cagcgccctg cgcgcgcgtt ccgccagtcc cgcgtagccc tccggcagcc
     5161 ggacgccacc ggccttggcc gtgtaggcgg tcaacaggat ggctgcctcc aggccggtat
     5221 cggaatccgg cgccatgaac accaagctgc cttccagcgt gtcgccgtcc tgcggctcgg
     5281 acaccagccc ctgtccaacc aggcgctggc cttgtttggc caattgctcg gccacgcccg
     5341 aaacctgggt acccacaggc tgccccatct gctgtaagag ccacagcgcc agcgcgcggt
     5401 gcagcagggg ctccttcgcg gcatggtcgc ggtagacctc gaggacgtgc tgccaattgg
     5461 cgtccggcag gaccacgccc aggctgtggc tggccagcca gtcggcgtaa taggcgtatg
     5521 tcgtaaggaa agcgttgccg cccgtcaggt cgccccacca gccgaacgta ccgttcaccc
     5581 ccgccatcat ggccaggcgc tggcgctggt tgcgcaggcg tgcctccaga tcctgtgtcg
     5641 atgagcctgc cggcttgccg gcggcgcgca gcgtgtcgta cgccagcgac agcggaatca
     5701 atcggctcga ggtttgctcg acgcagccat agggatagtc gatcaggtcg tccatcaccc
     5761 gcgcgaagcg gctggcggcg ttcgagacga atcgcacccg gacgtcctgg gcgtcggccg
     5821 gcagcgccaa cggcgccgcg ttcgtatcca atgccacgtc cgcctgccgc agatccagcc
     5881 atcccggcgt gaccaagccc actggcgtct gcagtcgatc caccggcttg ccgtccacct
     5941 cgaggcgggt atccaccatg ccgtcgccca atgccacgcg aggcaattgc aggtaattcg
     6001 cgccgggttt gagcgtgacc tggctatcca ccgacaggcc ggcaccgtcg accacccatc
     6061 gggccttttt ctcggtctgg ctctggttga acgccagcac gtcgaccgaa ggccggtcgc
     6121 cgcggcggaa atgcgtcggt ccgctccatt taaggtagaa gtccttgtcg gaacggatgt
     6181 aatcggtcct ttgccccacg tcgccctcgg tcgacaccgc ccgcaccgta atgcgccagc
     6241 gcgccagcga gtcgggcatg cgaaattgca tccgggtacg gccctgggcg tcagtgacga
     6301 gatcggcttg ccaggccgcg gtgtcgacct cgtcgcggcg cggacgctcc agcaccttga
     6361 cgccgcgctc gttgtactgg ccgcgctgcg gcccacctgg cgcgccgcgc atggccgaca
     6421 gcgccaggtc ataggtaatg aacgacaggc tcgacgaagt gcgcacattg ttgcggcgcg
     6481 ggtgatagaa gaactcggcg atgtccggcg caatctccgg ttgcagcaca tagaccatct
     6541 cgtcgaccac gctcacggtg aggtgcgccg caataggctt gcccccgaag gcgctttgca
     6601 ggtccagcgt gaccgtctcg cccggctggt agacgggctt gtcgctggcc accgtgagat
     6661 cgatggcggg cttggccacg acgatgcccg cgttctggaa ggcatattcg ccatcatgca
     6721 cgtagagtac cgagaacgtc atgttcggcg cgtagcctgg gccgaccttg atgcgagcct
     6781 tccattgcgt gggcgccacg cgttccaggt ctagccagtc gccaccggtc gaaagcaggg
     6841 ccttgccttc cacgctgtcg cgctccagag taagcagcgc atcgtccacc ggatacggaa
     6901 aggtgatcag cgcttcggcc gtatcgccga cgcggtagcg gggatggtcg aagacgattt
     6961 cgacgctgcc gggaaccgcc ttcagaccgt cgccggccac ccagtggctg gccgcggcca
     7021 acaggttgcc cgcggcatcc ttcaccgaca acatataaga gccgggagca tcgaactgca
     7081 cggggaaact cagccggcca tccggaccgg ccacaggcag cgaacctcgc ttcgtatcgc
     7141 gcgactccag gcgcagccac tcccaagtgg ccggcggatg cccggccgaa gcgccgccgg
     7201 acatggcggc cggcgtcaag ttgaacatca cgggttcacg cggcggcgaa aaagccttgt
     7261 ccgccgccag gcggtaggtc gtggcgccgc gcgcgatcag gatctcgcgg gtgacgcgta
     7321 cgcggtaggc cgcaccgtcc tgcgcaaaca gagtcagggc gtagcggctg gggtccttgg
     7381 caaccggtaa cgcgatgtcg gcattaccct gatcgtcggt ctgcagcgcc tgctgcgcca
     7441 gcttgaccgg aaacaggccg gcgtagcgca attccccatc ggcgattgcc accttttgcg
     7501 cgcgcaaggt tagggaaacc ttgccgttct tgaccggctt gccgtcgggg tagcgcagag
     7561 cgatcttgcc cttgggcgcc tcgcccgtgg cgtagtcggc cttgtccatt gtcaagttca
     7621 catcgaaatg cggcttgacg tagtcggcca cccggaaagc tgcgccatag aggctgccct
     7681 gataattgaa gcgcagcgta tagccgccgc ccggcgcgtt gtccggcagc gagaaacgcg
     7741 tgtcgccgcc cgagtccgcc gcgaagtcca cttggcgcgt ggccagcgga gcgccgttcg
     7801 gatccagtac ttccaacgca agcgtggccg gcacggccgc ggtcgagcgg ttggcgctct
     7861 ggaaggctct gccgatgaac ttcacgtgca ccgtatcgcc cggccggtac atgggacggt
     7921 cggtcaccgc gtagatcttg gtgttgtaga tctcgctgtc gtagtagaaa ttttccgaga
     7981 cgaatacgcc gccggcggcg tccgtgccca gcacgtaact gcgctcgggc gaaacgtggc
     8041 ccagcgcgag cgtcccgtcc gcgccggtgg cgcccttttg cagcacgccc acgccgtcgg
     8101 tccagtcgac caccgaaccc gccatgggct tgccattatc gcgtcgcgcg gtccagacca
     8161 gcaggccgct ggaggaggac ttcacgatcg ccaccgtgtc ggagacgaag agcagcgtat
     8221 gcgcccgata gttgccgata atggcttcca ccaaatacag gcccggggcc tgtttgccca
     8281 ccgggatcat cacattgccc agcgatgggg tgatgaagtt gctgctgctg cccgccaggt
     8341 tcacgtcctt gggcggtttg atggtcttgg cgtcccagat cggatagcga aagcgcgaga
     8401 ccatcggata cccccgcaag ggcgcaaagc gattgctcgg ttcgaagcgc gtgggcgcgt
     8461 tgggctgctc gcccagtttg aattccggct tggcctgcgt gacccggctg cgggtctcgt
     8521 aggaaaaaac ccgttgccag gcgtggcggg tctcgcccag ccagcggtcc cacaaaaagg
     8581 ccagcgtatt ggccaggcct tcgccctgat agttgggcgc gatgttcagg cggtgcaggt
     8641 tcttctgcgc tttgagaaag gcctgcggat ccggcacctt gtagaccacg atatccgcgc
     8701 cgccataggc ggcgagttcg gactggaatt cttcgccggg cgcctccagc cgcacctgcg
     8761 cgacctggtc gctgccgaag ctggcatcgg agagcaggaa aaagggctgt ccccgcacga
     8821 cttgatcgcc atagccgctg ggctgcgacg ccgccagcgt ggggttggcg tcgatactgt
     8881 cttgcgggga gacttgacca tcgtcggcct ggctatcgcc ttgggtggtt tccgctggcg
     8941 tggcgtcatc ggcccagacc accggactca gcgtcagggc ggcggccgcc agcaacaagg
     9001 caaagccgcg ggccagcagc cccctagcca gggggtgcgc gaggcgtcgg ccgccgggtt
     9061 cgcaatctgc gccgccgcgg ccggttccgc cctgcgcgtc ttccatgacg accatgctca
     9121 cggcctcaga aaagccagcc ggaatatacc ggcgaaattc ggattgccgt ccaacggccg
     9181 ccagcgagag tctttccatt gcatcaagtc ggaaacggcg accgcccgca gaccattgtc
     9241 ggtcggcgtg acggtacctg tgtggtaagc gatataacgg tctagccaga ccatgaggtg
     9301 ttggtcgtcg ccctgatcaa aaaacagcag atcaccgggc agcgcctggt tcacgtcgcg
     9361 cgagacaaag tggctgttgt cctggaccag agcgatggcc gaggcataag cgccgaccgt
     9421 gccgtcaaag cgcgtccagc gctgcgccaa agtgcgctgc ccggcgttca atgccaactc
     9481 ggggggcagg cggctcacat ccgattcgcc cagcatgcca ttggccttga gccagcgttc
     9541 gtcgtgcttg cgcaaggcct gcatggcggc aaagcgcacc aatcctgcgc aatcacgctg
     9601 ggtccagcgc ggcgtggggc cacggtccaa ttgcgcgtcg acgatgcgca cgaaccaggc
     9661 gcgaaaaatg gccgattgcg ccggcgacag tgcctgcgcg tcgtccggtt ctccggaagc
     9721 ggtcagcgcc cgcgccaggc cgggcagcaa gccgcccgcc gccgcagcgc ccaatgcggc
     9781 atggcatctc aggaactcgc ggcggcgcat caattctccg acgcggccgc gtcgtcgccg
     9841 aaaccactgt catcgacggg cgaccgatgc ggcgggaaca tggcggcatc gtcgcgcggg
     9901 acgccgcccg ccttggcgtt ggctcgccac gtcacgggca cccagcccgc ggcgctcggc
     9961 agcggcgagt ccggcagact cagcgaaacc ggcgcataag cggcgaaagc atgcagcttg
    10021 ggcagcaggt gctggcgcgc ggcattgcgg aagatggcct cctggtccgc gggcaaggtc
    10081 cggcccgcct cgcgctcggc cagcgccgcc aggctggacg gcgtgatcac cgccaccgtg
    10141 gcgcgcgccg gctgcggcgc gagcgtatcg gcctcggacg gatagcgctt gtcgagcacg
    10201 gccagcgtgt cgtccaccag gcgcgcatcg ggcgagaaca cggcatagcc atgcgccagc
    10261 gccaaggtca ccgggaaata gcgcggcgtg gaaagctgct cggcgtacgg cgcggcccgg
    10321 ctgctggcgg taccggcgcg cgcgctcacg ggtcgcatcc agcacgagac gccttcggac
    10381 gctttgcagg cctgaaccgg caggcgccga tatccgggcg agtcgccggc cctggcttca
    10441 taggcaccga tgacgcggtc gaagagttgg gccatggcgc cggaaaaagc ggcgttcccc
    10501 atggcatcct tggtcagccg ggccacgaag accggcgaag ccagcgtgga tgctgcatac
    10561 cagcacaccg ctgcgggccc ttccagatgg tcgtgcaagg ccttggccga tccggccagg
    10621 tccggtccca gcttctccag cagtccggcg gcgtcattcc aatccaccgg cagggtcgca
    10681 caggcggccg cgttggccgg caaggcctgc catagcgctt cgccattcca ctgctgcggc
    10741 aaccgcgcgg gatcgatcaa ggcagccgtg ctccaggcgc tttccccgcc ggcctgagcc
    10801 ggcgcggcat gcgcctggcc gaaatcgaac cgaaccgcgt cgatgccgga gaaaaaggcc
    10861 tggtagccga aggacagata gttggccgag atagtgatcc ggtgcccgtc ggccacggcc
    10921 ggcgcc
//
LOCUS       DASWPO010000277         8673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2386817, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000277 DASWPO010000000
VERSION     DASWPO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386817"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1212)
                     /locus_tag="VGC69_14545"
     CDS             complement(280..1212)
                     /locus_tag="VGC69_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296544.1"
                     /translation="MDLRQLEYFVHVAELGSFTKAAAILDVAQPALSRQVRRLEIELR
                     QTLLYRNGRGVTPTEAGKRLLAHGRGILHQFERARQEVEQARGAPVGRIVVGVPHSIS
                     QLITAPFATAFRAAFPEATVCITEGLTLHLQEWLLSGRIDVAILHDPVPTPSLEITPL
                     REDPLFLIERRHSRGGEPKPVPVKALADFPLIIPSRPHPLRMMIETRLANLGRQLSVA
                     IEVDAVGGIVDLVSQGLGHAIVTQNALLIAPDAARLSARRIVSPRLSSALVLATSAER
                     PSTELARRTTGLIREVLSERLAQAARASRSAPPR"
     gene            complement(1389..3269)
                     /locus_tag="VGC69_14550"
     CDS             complement(1389..3269)
                     /locus_tag="VGC69_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296545.1"
                     /translation="MPGMKKTPPSHFIDRLFERIIDPFRPSEDAMPPSGLAGFYIHYL
                     RQVKGPLFFLLVVGLFAALLDVALFDFLGRIVDWASNAPSSSFFETHGPMLWLMAFVT
                     LILRPVLTGLHDLLVHQTISPSLTTLVRWQNHRYVLKQGLAFFLNDFAGGIANRILQT
                     GIALRDSAVSAVDAIWHVLIYSVTALVLFAEADWRLTLPLIIWIVGYIASLIYFVPRV
                     KQRATIASGSRGKLMGRIVDGYTNIGTLKLFAHSQFEEQYVREAMVEQTEQAQYAARM
                     ITGMGFVIAILNGILIAGTPGLALWLWSSGRMSAGVIALATGLAIRINNMSGWIMWVV
                     NGIFENLGTVQDGVHTISRPRIVADAPGAQDLRVTQGGGIRFDDVSFHYGKQAGVIDH
                     LDLAVRPGEKIGLVGPSGAGKSTLISILLRLYDLDGGRILIDGQDIAQVKQESLRSQI
                     GVVTQDTSLLHRSIRDNMLYGKPDASDEDIMRALRQAQADGFVASLTDLQGHSGLDSH
                     VGERGVKLSGGQRQRIAIARVLLKNAPILILDEATSALDSDAESAIQENLETLMRGKT
                     VIAIAHRLSTIARLDRLVVMERGAIVEMGTHAELVAKDGLYARLWRHQSGGLLDGPSG
                     NK"
     gene            3417..4367
                     /gene="epsC"
                     /locus_tag="VGC69_14555"
     CDS             3417..4367
                     /gene="epsC"
                     /locus_tag="VGC69_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEY0296546.1"
                     /translation="MSASSRVPAAHWNLDGIVSGLRSARLDWRGPHGRLREESAGREF
                     PSQDSLRQIVAQLCGALFPMRLGPIDLREESEDFYVGHTIGEALDTLLQQAYLELRHL
                     GRHAPQTHEETMARAVEIVRQFGAELPAVRRALDLDVTAAYQGDPAARSVDEVLLCYP
                     GVTAMIHHRLAHVLYRLGAPMLARIVAEIAHAETGIDIHPGATIGASCFIDHGTGVVI
                     GETAIIGDRVRLYQMVTLGAKRFPPGENGELKKGLPRHPIIEDDVVIYAGATILGRIT
                     IGHGSTIGGNVWLTRDVPPDSNVTQASLVSNMPGCGLDGG"
     gene            complement(4386..5135)
                     /locus_tag="VGC69_14560"
     CDS             complement(4386..5135)
                     /locus_tag="VGC69_14560"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296547.1"
                     /translation="MTGTATDRIRHALQDEIADARLPPGQALDEAALARRFQVSRTPV
                     REALLQLAVQGFVRVLPRAGIFVAALDLRELTDLSETLAHLDGLCAGLACQRMRTPER
                     RKLAALLQSGRQARDEGDTARYAVLDENLHNAIYQNCDNPFLMRQVQLIRRRTQPYRI
                     RHLDKQPERLRQSHQEHDEIVTAILDDTPQAAAEAASRHVLNASRHLNDLASRQPEAL
                     WPSERAKRARTPSPDAPRLTLDWVFSGEPAA"
     gene            5242..6072
                     /locus_tag="VGC69_14565"
     CDS             5242..6072
                     /locus_tag="VGC69_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEY0296548.1"
                     /translation="MDKLSESLQKLRDFLREKRLFVLTGAGCSTDSGIPDYRDDAGEW
                     KRRPPIDFQTFMGSDLARARYWARGMVGWRRFGQVRPNAAHRALAAMERAGAVVLLVT
                     QNVDGLHQAAGSRAVVDLHGRMDEVHCMNCDWRGPRKDWQEQLEELNPAWADLDAPDA
                     PDGDADLEGVDFSRFVVPACPRCGGIVKPDVVFFGELVPRDRYERAMAALDEAGAVLV
                     VGSSLMVHSGYRYVKEASQRGLPIAAINLGRTRADDLLALKVQQSCSQVLGALYPVPA
                     "
     gene            complement(6095..6955)
                     /locus_tag="VGC69_14570"
     CDS             complement(6095..6955)
                     /locus_tag="VGC69_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0296549.1"
                     /translation="MSRPFSRDDTRRLAMILAEVAQSVVMPRFRHLPPEAIRSKSTPR
                     DLVTDADEEAERQIFARLSKLHPGAVLIGEEASERNPALLNMLVDADLAFLVDPIDGT
                     RNYVAGLPLFGMMIAACHRGEVIAGVIYDPICRDAAMAVRGEGAWLGREDGSTQALVA
                     AKPVPLEDMDSMIDTSSLPKSLQDMANSRLSTFARNASLHCAAHEYRMAASGYCHVLL
                     YNNLHPWDHAAGWLLHQEAGGYSAHFDGSPYRPTHRTGGLLVAPDRGSWEAIHKLLLV
                     DRRHSSRRTL"
     gene            complement(7151..8614)
                     /locus_tag="VGC69_14575"
     CDS             complement(7151..8614)
                     /locus_tag="VGC69_14575"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0296550.1"
                     /translation="MSSTLNLKRPDLLRDACYIDGAWVKGSESFPVVNPATGRVILSV
                     PKLGRKETEQAIASAKAAQAPWAAKTAKERAAVMLKWAQLMLANQQDLAAIMTAEQGK
                     PLPEAIGEVAYAASFLEWFGEQAKRVDGDVLQSPKAGQRLLALKQPIGVTAAITPWNF
                     PSAMITRKAGPAIAAGCAMVLKPAEQTPLSALALAVLAEEAGVPKGVFHVITGDAPEI
                     GGALCDSDVVRKLSFTGSTEVGRLLMKQCAPTIKKLSLELGGNAPFLVFDDADPDAAV
                     EGILASKYRNAGQTCVCANRIYIQSGIYEEIVKRLTEKVQGTLKVGDGSEPGVNQGPL
                     IDEAAIAKVKEHIADATAKGAKLVTGGKPHALGGTFFEPTILRDVTQQMKVATEETFG
                     PVAPLFKFDTEQEALRMANDTIFGLAAYLYTRDYARVWRVSEALEYGIVGINTGIISN
                     EVGPFGGVKQSGLGREGSKYGIEDYLETKYLCMDLGK"
BASE COUNT         1304 a         2705 c         3195 g         1469 t
ORIGIN      
        1 ggttcttccc ggtgggggcg cgcgcccggc accgcgacga ataccgggcg gttcgcttct
       61 tcggggggcc aagggcggtc ggggcggtgc gcctcccaga ggtgcacctg ggtgtcgatg
      121 atcagcatat tgtctccagc ggcgtccagc ggtctcggac gcctgtttgt aagctgatgt
      181 atcttatgcg cagccagggt agaacggaat ggcattgccg atctagctgt tattacaaga
      241 atgaatgaca gcgccagccg ccgcgctgcg ccgccgggct cagcgcggcg gcgcgctccg
      301 ggaggcgcgc gccgcttgcg ccagccgctc ggacaggact tcgcggatca gcccggtggt
      361 gcggcgcgcc agctcggtgc tgggccgctc ggccgaggtc gccagcacca gcgcgctgct
      421 caggcgcggc gagacgatgc ggcgcgccga cagccgggcc gcatccggag cgatcagcag
      481 cgcgttctgc gtgacgatgg cgtgccccag tccctgggag accagatcga cgatgccgcc
      541 caccgcatcg acctcgatcg ccaccgagag ctggcggccc aggttggcca ggcgcgtctc
      601 gatcatcatg cgcagcggat gcggccggct ggggatgatg agcggaaaat cggccagcgc
      661 cttgacgggc acgggcttgg gctcgccgcc gcggctgtgg cggcgctcga tcaggaacag
      721 cgggtcttcg cgcagcggcg tgatctccag gctgggggtg ggcaccgggt cgtgcaggat
      781 ggccacgtcg atgcgcccgc tgagcagcca ttcctgcaag tgcagcgtca gcccttcggt
      841 gatgcacacg gtggcttcgg gaaaggccgc gcggaaggcc gtggcgaagg gcgcggtgat
      901 caactggctg atggagtggg gcacgccgac cacgatgcgt cccaccggcg cgccgcgcgc
      961 ctgttcgact tcctggcgcg cccgttcgaa ctggtgcagg atgccgcggc catgggcaag
     1021 caggcgcttg ccggcttcgg tgggcgtgac gccgcggccg ttgcggtaga gcagcgtctg
     1081 gcgcagttcg atctccaggc ggcgcacttg ccggctgagg gccggctggg ccacgtcgag
     1141 tatggcggcg gccttggtga agctgccgag ttcggcgacg tggacgaagt attcgagttg
     1201 gcgcaggtcc atgtggcgat ggctgaagtt gcgggtagtc tgttgtaccc cagttgggtg
     1261 gggggcttgg ccgcgaactt gcgcttcgaa ggcccgggcg ctctgcccgc ccactgtacg
     1321 gtggtggtcg cccgcatggc gggcatggcg tgagatagat ggatccctag gcctgggatg
     1381 gcacggattt atttattccc ggacgggccg tccaacagcc cgcccgattg gtgccgccat
     1441 agacgagcgt agagcccgtc cttggcgacc agctcggcgt gcgtgcccat ttcgacgatg
     1501 gcgccgcgtt ccatcaccac caaccgatcc aggcgggcga tggtcgacag gcggtgggcg
     1561 atggcgatca ccgtcttgcc gcgcatcagg gtttcgaggt tttcctggat ggcggattcg
     1621 gcatcggagt ccagcgccga ggtggcctcg tccaggatca ggatgggggc gtttttgagt
     1681 agcacgcggg cgatggcgat gcgctggcgc tggccgccgg agagcttcac gccgcgctcg
     1741 cccacgtgcg agtccaggcc gctgtggcct tgcaggtcgg tgaggctcgc gacgaagccg
     1801 tcggcctgcg cctggcgcaa ggcccgcatg atgtcctcgt cgctggcgtc gggcttgccg
     1861 tagagcatgt tgtcgcggat ggagcggtgc aggagcgagg tgtcctgcgt gaccacgccg
     1921 atctgcgagc gcaggctttc ctgcttgacc tgggcgatgt cctggccgtc gatgaggatg
     1981 cggccgccgt ccaggtcgta caggcgcagc aaaatgctga tgagcgtgga cttgcccgcg
     2041 cccgaggggc cgaccaggcc gatcttctcg ccgggccgga ccgccaggtc gaggtggtcg
     2101 atgacgccgg cctgcttgcc gtagtggaag gacacgtcat cgaagcggat gccgccgccc
     2161 tgcgtcacgc gcaggtcctg cgcgcccggc gcgtcggcca cgatgcgcgg gcgcgagatg
     2221 gtgtggacgc cgtcctgcac ggtgccgagg ttttcgaaaa tgccgttgac cacccacatg
     2281 atccagcccg acatattgtt gatgcggatg gccaggccgg tggcgagcgc gatcacgccg
     2341 gccgacatgc ggccgctcga ccacagccac agcgccagcc cgggcgtgcc ggcgatcagg
     2401 atgccgttga ggatcgcgat cacgaagccc atgccggtga tcatgcgggc ggcgtactgg
     2461 gcctgctcgg tctgttcgac catggcctcg cgcacgtatt gctcctcgaa ctggctgtgc
     2521 gcgaagagct tgagcgtgcc gatattggtg tagccgtcga cgatgcggcc catgagcttg
     2581 ccgcgcgagc ccgaggcgat ggtggcgcgc tgcttcacgc gcggcacgaa gtagatcagc
     2641 gaggcgatat agcccacgat ccagatgatg agcggcaggg tcaggcgcca gtcggcctcg
     2701 gcgaacagca ccagcgcggt gaccgaatag atcagcacgt gccagatggc gtccaccgcc
     2761 gagacggccg agtcgcgcag cgcgatgccg gtctgcagga tgcggttggc gatgccgccg
     2821 gcgaagtcgt tgaggaagaa ggccaggccc tgcttgagca cgtagcggtg gttttgccag
     2881 cgcaccagcg tggtgaggct gggcgagatg gtctggtgca ccagcaggtc gtgcaggccg
     2941 gtgagcaccg gccgcaggat cagcgtgacg aaggccatca gccacagcat ggggccgtgg
     3001 gtctcgaaga agctgctgct gggagcgttg ctggcccagt ccacgatgcg gcccaggaag
     3061 tcgaacagcg ccacgtccag cagcgccgcg aaaaggccga cgaccagcag gaagaacagg
     3121 gggcctttga cctggcgcag atagtggata tagaacccgg ccaggccgct cggcggcatg
     3181 gcgtcctcgg atggcctgaa ggggtcgatg atgcgttcga acagacggtc gatgaagtgc
     3241 gagggagggg tcttcttcat gcctggcatg ggagtagggg tgtccgggtg atagtagcaa
     3301 cttccgccta aaatgcgtca gcgcctgtta aggtgcctgg tcttctttgt tcgaggtttt
     3361 gtcatgttcc attacaccca gggtcgccac cacgcggcag catcggagcc gcggtcatga
     3421 gcgcgtcgtc cagagtcccg gcggcgcact ggaatctcga tggcatcgtg tcgggcctgc
     3481 gctcggcgcg gctcgactgg cgcgggccgc acggccggct gcgcgaagag tcggccggcc
     3541 gcgaatttcc ttcgcaggac agcctgcgcc agatcgtcgc gcagctgtgc ggggcgctgt
     3601 ttcccatgcg gctgggtccc atcgacctgc gcgaggaaag cgaagacttc tacgtgggcc
     3661 acaccatcgg cgaggcgctg gatacgctgt tgcagcaggc gtacctggag ctgcgccatc
     3721 tcggccgcca cgcgccgcag actcacgaag agaccatggc gcgcgccgtg gagatcgtgc
     3781 gccagttcgg cgccgagctg cctgccgtgc gccgcgcgct cgacctggac gtgacggccg
     3841 cctaccaggg cgacccggcg gcgcgcagcg tcgacgaggt gctgctgtgc tatccgggcg
     3901 tcaccgccat gatccaccat cgcctggccc acgtgctgta ccggctgggc gcgcccatgc
     3961 tggcccgcat cgtggccgag atcgcgcacg ccgagaccgg catcgacatc catcccggcg
     4021 cgaccatcgg cgcgagctgc ttcatcgacc acggcacggg cgtggtgatc ggcgagacgg
     4081 ccatcatcgg cgaccgcgtg cggctctacc agatggtgac gctgggcgcc aagcgcttcc
     4141 cgccgggcga gaacggcgag ctcaagaaag gcctgccgcg ccaccccatc atcgaggacg
     4201 acgtggtgat ctacgccggg gccaccatcc tgggccgcat caccatcggc catggatcga
     4261 ccatcggcgg caatgtgtgg ctgacgcgcg atgtgccgcc ggacagcaac gtgacgcagg
     4321 ccagcctggt cagcaatatg ccgggctgcg ggctggacgg cggttgagcc gcgccgcctg
     4381 aggcatcagg cggctggctc gccggagaag acccagtcca gagtgaggcg cggggcgtcc
     4441 ggcgacggcg tcctggcgcg cttggcgcgt tcgctcggcc acagcgcctc gggctggcgc
     4501 gaggccaagt cgttcaggtg gcgggaggcg ttgaggacgt ggcggcttgc ggcctcggcc
     4561 gccgcctggg gggtgtcgtc gaggatggcg gtcacgatct cgtcatgttc ctggtgcgac
     4621 tggcgcagcc gttcgggctg cttgtccagg tggcgaatgc gatatggctg ggtgcggcgg
     4681 cggatgagtt gcacctgccg catcaggaag gggttgtcgc agttctggta gatggcgttg
     4741 tggaggtttt cgtcgagcac ggcatagcgc gccgtgtcgc cctcgtcgcg ggcctgccgg
     4801 ccgctttgca gcagcgcggc cagcttgcgc cgctcgggcg tgcgcatgcg ctggcaggcc
     4861 aggcccgcgc acaggccgtc caggtgggcc agggtttcgc tcaggtcggt cagctcgcgc
     4921 aggtcgagcg ccgcgacgaa aatgccggcg cgcggcagca cgcgcacgaa accctggacg
     4981 gcaagctgca gcagggcctc ccgcacgggc gtgcgcgaaa cctggaagcg cctggccagg
     5041 gccgcctcgt ccagggcctg gccgggcggt agccgggcat cggcgatctc gtcttgcagt
     5101 gcgtgtcgga tacgatcggt ggcggtgccc gtcatggccc ggggttcctt gaggtcgttg
     5161 cgatgagccg gacccattat catgagcggc gctcatgaat caacaaatcg gcatctactg
     5221 cgtagcatag ggcttgtatc gatggataag ctttcggaat ccttgcaaaa actgcgtgat
     5281 tttttgcggg aaaaacgtct tttcgtgctg actggcgccg gttgcagcac ggattcgggc
     5341 atcccggatt accgcgacga cgcgggcgaa tggaaacgcc ggccgcccat cgatttccag
     5401 accttcatgg gcagcgacct ggcgcgtgcg cgctactggg cgcgcggcat ggtgggctgg
     5461 cggcgttttg gccaggtgcg gcccaatgcg gcgcaccgtg cgctggccgc gatggagcgg
     5521 gccggcgccg tcgtgctgct ggtcacgcag aatgtcgacg gcctgcacca ggcggccggc
     5581 agccgcgcgg tggtcgacct gcacggccgc atggacgagg tgcattgcat gaactgcgac
     5641 tggcgcggcc cgcgcaagga ctggcaggaa cagctggaag aactgaatcc cgcgtgggcc
     5701 gacctggatg cgcccgatgc ccccgatggc gatgccgatc tcgagggcgt ggatttttcg
     5761 cgctttgtcg tgccggcctg tccgcgctgc ggcggcatcg tcaagccgga cgtggtgttc
     5821 ttcggcgaac tggtgccgcg cgaccgctac gagcgcgcca tggccgcgct cgacgaggcc
     5881 ggcgcggtgc tggtggtggg ctcatcgctc atggtgcatt cgggctaccg ctacgtgaag
     5941 gaagccagcc agcgcggctt gccgatcgcg gccatcaacc tgggacgcac gcgcgccgac
     6001 gacttgctgg ccctgaaagt gcagcagtcc tgttcgcagg tgctgggggc gctgtacccc
     6061 gtgccggcct gagccggcac ggtatccggc aaggctacag cgtgcgccgg ctgctgtgcc
     6121 ggcggtcgac cagcaatagc ttgtggatgg cttcccagct gccgcggtcc ggggcgacca
     6181 gcaggccgcc ggtgcggtgc gtgggccggt aggggctgcc gtcgaaatgg gccgaatagc
     6241 cgccggcttc ctggtgcagc agccagccgg ccgcgtggtc ccagggatgc aggttgttgt
     6301 agagcagtac gtggcagtag cccgacgccg ccatgcggta ctcgtgcgcg gcgcagtgca
     6361 gcgaggcatt gcgggcgaag gtcgacaggc ggctgttggc catgtcttgc aggctcttgg
     6421 gcaggctgct ggtgtcgatc atgctgtcca tgtcttccag cggcacgggc ttggcggcca
     6481 ccagcgcttg cgtgctgccg tcctcgcggc ccagccaggc gccctcgccg cgcacggcca
     6541 tggcggcgtc gcggcaaatg gggtcgtaga tgacgccggc gatgacctcg ccgcgatggc
     6601 aggcggcgat catcatgccg aagagcggca gccccgccac gtagttgcgg gtgccgtcga
     6661 tggggtcgac caggaaagcc aggtcggcat cgaccagcat attgagcagc gcggggttgc
     6721 gctcggaggc ttcttcgccg atgagcacgg cgcccggatg cagcttggac aggcgcgcga
     6781 agatctggcg ctccgcttcc tcgtcggcat cggtgaccag gtcgcgcggc gtgctcttgc
     6841 tgcgaatggc ctcgggaggc aggtggcgaa agcgcggcat gacgacggat tgcgccacct
     6901 cggccaggat catggcaagg cggcgggtgt cgtcgcgcga aaaaggtcgg ctcatgtcgg
     6961 gaagggaaga ccaggaaggc ccaccaatct agcacgacaa accgccggcg tgtgatgtga
     7021 tgattacagc caaattgccg aatcatgagc cgcggacccc ggaggaatcc gaagcgcagc
     7081 ccctttccag gggcgagcat cggaattccg cagggcagcc gcggcggctc gagaacccgg
     7141 ttttgtcggc ctacttgccc aggtccatgc acaggtattt ggtctcgagg taatcctcga
     7201 tcccgtactt ggacccttcg cgtcccaggc ccgactgctt cacgccgccg aacggaccca
     7261 cctcgttgga gatgatgccg gtgttgatgc cgacgatgcc gtattccagc gcttccgaca
     7321 cgcgccacac gcgggcgtag tcgcgcgtgt acaggtaggc ggccaggccg aaaatggtgt
     7381 cgttggccat gcgcagcgcc tcttgctcgg tgtcgaactt gaaaagcggc gccaccgggc
     7441 cgaaggtttc ttcggtggcc accttcatct gctgcgtgac gtcgcgcagg atggtgggct
     7501 cgaagaaggt gccgcccagg gcgtggggct tgccgcccgt gaccagcttg gcgcccttgg
     7561 ccgtggcgtc ggcgatgtgc tccttcacct tggcgatggc cgcctcgtcg atcagcgggc
     7621 cctggttgac gcccggctcg gagccgtcgc ccaccttgag cgtgccttgc accttctcgg
     7681 tcaggcgctt gacgatttcc tcgtagatgc ccgactggat gtagatgcgg ttggcgcaga
     7741 cgcaggtctg gccggcgttg cggtacttcg aggccaggat gccctcgacc gccgcgtccg
     7801 ggtcggcgtc gtcgaacacc agaaagggcg cgttgccgcc gagctcgagc gacagcttct
     7861 tgatggtagg ggcgcattgc ttcatgagca gccggcccac ttcggtcgaa ccggtgaagc
     7921 tgagcttgcg caccacgtcg ctgtcgcaca gcgcgccgcc gatctcgggc gcgtcgcccg
     7981 tgatgacgtg gaacacgccc ttgggcacgc cggcctcttc ggccagcacg gccagggcca
     8041 gggccgacag cggggtctgc tcggcgggct tgagcaccat ggcgcagccg gcggcgatgg
     8101 cggggccggc cttgcgggtg atcatggccg aggggaagtt ccagggcgtg atggcggcgg
     8161 tgacgccgat gggctgcttg agcgccagca ggcgctggcc ggccttgggg ctttgcagca
     8221 cgtcgccgtc gacgcgcttg gcttgttcgc cgaaccactc caggaaggag gcggcatagg
     8281 cgacttcgcc gatggcctcg ggcaggggct tgccctgctc cgcggtcatg atggcggcca
     8341 ggtcctgctg gttggccagc atgagctggg cccacttgag catgacggcg gcgcgttcct
     8401 tggcggtctt ggcggcccat ggcgcttgcg cggccttggc gctggcgatg gcctgctcgg
     8461 tttccttgcg gcccagcttg ggcaccgaca ggatgacgcg gccggtggcc gggttgacga
     8521 cggggaagct ttcgctgccc ttgacccatg cgccgtcgat gtagcaggca tcgcgcagca
     8581 ggtcggggcg tttgagattg agtgtggatg acatggtgtt gtgcgtctcg gaaaaagagg
     8641 cgggcggcca gcttgtcgcc gagcgcggcg gcg
//
LOCUS       DASWPO010000278        44983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1047397, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000278 DASWPO010000000
VERSION     DASWPO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 44983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44983
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047397"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /gene="cyoA"
                     /locus_tag="VGC69_14580"
     CDS             complement(<1..712)
                     /gene="cyoA"
                     /locus_tag="VGC69_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEY0296551.1"
                     /translation="MKIPSLATIARTLAVGALVLLSGCKVEVLDPKGDIGMQEKHLLL
                     VALGLMLIVVIPVIIMTIAFAWRYRSTNTRATYAPRWSHSTAIEAVVWTIPCIIIAIL
                     ATITWTSTHELDPYKELKSDVKPINIDVVSMDWKWLFIYPDYNIATVNQIAFPVDTPV
                     NFRITSATVMNSFFIPQLGSQIYSMSGMQTRLSLIAREAGTYAGLSANYSGGGFSGMR
                     FSAIATTQQGFDEWVAKAK"
     gene            complement(930..1484)
                     /locus_tag="VGC69_14585"
     CDS             complement(930..1484)
                     /locus_tag="VGC69_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0296552.1"
                     /translation="MTLPGPGLLIDDDELYLRTLQRGLSRHGLETHLACSAAEALRLA
                     ESVRPAFALVDLRLGEDSGLALIAPLRALRADMRILLVTGYASVATAVNAIKRGADDY
                     LPKPATVPMILRTLGLAQAREVVIEEAMIPLHRLEWEHIHQALSETGGNVSAAARLLG
                     MHRRSLQRKLAKKPGPERDPSADD"
     gene            complement(1481..2707)
                     /locus_tag="VGC69_14590"
     CDS             complement(1481..2707)
                     /locus_tag="VGC69_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0296553.1"
                     /translation="MTGRPAHSFLRTLCVLRWVAIAGQAVAILVASGPLDVSLPLLPL
                     WGGVAALVLFNLVATVLPQRSEELAPGVAFMHLMVDMLVLTWMVAWSGGIGNPFGMMF
                     LVLTALGALALPRSWALLAALVSVAGYSVAAVFGQPLVSPHDEHTLILWGMAATFLIS
                     VAVVCSFLTRLAAGLRSRERELAALRERFTRNEGIVALATHAAAMAHELNTPLATMTL
                     LADEIADQAQGGQAPGAQMRSDISTLIELLALCRERIRNLAVPDQVDLVRVVGQWTLV
                     RPAIELHRAGELPASLRVEPAIAHLLQALLNNAADAAEEAGDLRVELHLSYQDGILRG
                     EVRDHGAGIDPERPLLPASGLFHSGKPQGLGVGLALSHATVEQLGGEMTLAGAPGGGT
                     RIRFVLPLHKQSGISS"
     gene            3060..5309
                     /gene="ggpS"
                     /locus_tag="VGC69_14595"
     CDS             3060..5309
                     /gene="ggpS"
                     /locus_tag="VGC69_14595"
                     /EC_number="2.4.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033828 - glucosylglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerol-phosphate synthase"
                     /protein_id="HEY0296554.1"
                     /translation="MFLATDLDGTFLAGDPEDRLRLYQTIVSHPEIQLVYVSGRSLEH
                     ILPLLDDPSLPNPDYIIADVGATIVHGDTLQPVQPLQTRLETRWPGEQAVTEALSAFP
                     WIKRQDVPQSRRCSYYCEPCRSDDPDLRQAVEALGCDLLYSADKYLDVLPRGVDKGNT
                     LRALVEHLELDPDAILVAGDTLNDASMYREGFIGVCVGDSEPALLAQTGNSARVLHAA
                     RRGCGGILEAFAYFGFLGEQGVEAELPQALPPGGSDLVIVYHRLPYEEKKVNGAIQRR
                     RPTSPNGIIPTLLGFFDDGQPGSWVAWAEHEDTDGEFEVHTAVDTRRYPRLTAARVAL
                     SKADVDVFYRRFSKEAFWPTLHTFWERARFNEDDWQVFLRVNRMFAERTAAEAAHGAV
                     VWLHDYNLWMVPAYLRELRPDLRIAFFHHTYFPSADVFNVLPWRRSIVGSLLQCDYIG
                     FHIPRQVENFVDVAAGVMPIRRAQRQNCAPRFLTYGCAVGLESVTTVIETETRTVRLG
                     AHPVGLDVGRVRRTLALASVQENMRKLRAELSRQRVILSIERLDYTKGTLEKLCAFER
                     MLDENPELLGKVSLVCVCVPAAREMTIYDTLQVQIEQAVGRINGRYSQVGWMPLHFYF
                     RPLPFEEVIAWYAMADVMWITPLRDGLNLVSKEYVAVQGLLNGQGVLVLSEFAGAAAE
                     LKGALLTNPHDQVDLVRACYTALNLPAAEARARLRELFEIVSTHDVRRWGQEFLAAVN
                     EQPEAAPAG"
     gene            complement(5325..6089)
                     /locus_tag="VGC69_14600"
     CDS             complement(5325..6089)
                     /locus_tag="VGC69_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0296555.1"
                     /translation="MTQASQIAELRERGFVVARGFADAATVAALRTLAQRHLDEAIAP
                     LEYEADLQYPGAPASRQAAGGHTVRRLLDAYGRDPLFARWATSPRIGQWLAAYFGEAP
                     VLSTVHHNCVMTKHPSYGSLTGWHQDIRYWSFSAPELMSCWLALGPETADNGGLFFLP
                     GSHAADFSSAQFDEKKFFLPDPPGNQRWIAQAVCPELEPGDVVFFHCRTLHAAQRNAG
                     DAVKFSLVHTYYPGRIQPLPGSRSASRPGVPLAARP"
     gene            complement(6086..7186)
                     /locus_tag="VGC69_14605"
     CDS             complement(6086..7186)
                     /locus_tag="VGC69_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0296556.1"
                     /translation="MSPVLPASLIARWLLLLVLLAGVYFLSGFLVPALAALIIGLATW
                     PLYRRLLVSCRQNTTVGATVALLLVLLVVVVPLSLASIYAFREASHLFAWMLAANRDG
                     AAVPSWMPTIPLVGDRLATYWRTYLGEPHALGDLVQLISGEHIGNIYRMVLGAAGNAV
                     QLLLAVLFMLLTLFFIYKDGQRLLGQIDLLGERILPARWQRFSRMVPATIGATVTGMS
                     LIAIGEGVVLGAAYWIAGVPAHVLLAVITGFMALVPGGAPTAFTLVSLYLIGANHIAA
                     GIGLLIWGAVELFIVDKTLRPRLVGGPVKLPFLPTFFGLVGGLKTLGIVGLFVGPVLM
                     ALLVSVWREWVRTVAEERDAELPRPPGGEGRP"
     gene            complement(7294..8325)
                     /locus_tag="VGC69_14610"
     CDS             complement(7294..8325)
                     /locus_tag="VGC69_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296557.1"
                     /translation="MDRFQAMQVFSRVVEANSFTRAADALSLPRTTVTTIIQNLEKHL
                     GVRLLNRTTRRLSLTPDGAAYYKHSVRILAEVEETEAYLQDVSAHPQGRLRIDVKPSI
                     GTMVLIPALCDFHTRYPDVELAIGLSDRKIDLVQDAVDCVIRAGDLEDSSLVARRIGT
                     VELITCAAPAYLERYGVPKTLDDLKRHQAVHYFLGPGRNHGWQFMVDGQPRQVELPGT
                     VSVNEWSAHLACGVQGFGMIQTARFMALAHMYKGELVEVLPEYRPAAMPISLLYLQSR
                     QLSPKVRAFSDWVAELFAGCPLLSGRDENDPSMLECKRLMAEGARISPQAHEATRRHV
                     PIDPSGVLV"
     gene            8588..9766
                     /locus_tag="VGC69_14615"
     CDS             8588..9766
                     /locus_tag="VGC69_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0296558.1"
                     /translation="MTVSRPRAFRLPAFRPRFPALTLAILLTATGGYAVFGPAAQTPA
                     LAQPAPPAGVPVDVAPAIGRSIVDWQRYSGRLEAIDRVDIRPLVPGTLTQVHFKDGAL
                     VKKGDPLFTIDPRPYEAEVERAEARAAYTVADLSRAKRLLAENAIARRDYEEKENAAR
                     DADASLKTARINLGYTRIVAPVDGRVSRAEVTVGNVVSAGASSAVLTTVVSVARMYAA
                     FDVDEQTFLRYVNPGRAKGTPVPVQMGLADEDGYSREGVVESVDNRLDPASGTIRVRA
                     VFDNKDGLMLPGLYARVRLGGSTPHDAVLVDEKAIGTDQNKRYVLVVDDANHANYREV
                     QLGTSVGPLRVVTQGLKTGERIVVNGLQRLRPGDVVAPSAVSMDDRTSAIKTASASLK
                     "
     gene            9795..12989
                     /locus_tag="VGC69_14620"
     CDS             9795..12989
                     /locus_tag="VGC69_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY0296559.1"
                     /translation="MNISKFFIDRPIFAGVLSVLVLLGGLLAMFQLPISEYPEVVPPS
                     VVVRAQYPGANPKVIAETVASPLEQSVNGVEDMLYMQSQANSDGSFALTVFFRLGTDP
                     DKAQQLVQNRVSQALPRLPDDVQRLGVTTVKSSPTLTMVVHLISPDNRYDATYLRNYA
                     VLNVKDRLARLNGVGEVQVWGAGDYAMRVWLDPNKVAQRDLTATEVVNAIREQNVQVA
                     AGIIGASPTASDVPMQFSVNARGRLQTEEEFGQIILKSSPDGAVTHLSDVARIQLDAQ
                     EYALRSLLDNKQAVAMAIMQAPGSNALDVSHQVREAMAELSQDFPPSVQYRIEYDPTQ
                     FVSASIEAVVHTLLEALVLVVLVVIVFLQTWRASIIPLLAVPVSIIGTFSLLLLFGYS
                     INALSLFGMVLAIGIVVDDAIVVVENVERNIEAGLSPREATYRAMREVSGPIVAIALT
                     LCAVFVPLAFMTGLSGQFYKQFAVTIAISTVISAFNSLTLSPALSALLLKPHDEQTDW
                     LSRAMNKVLGPFFRGFNRLFGRASNRYAAGVSGVLRRKGSSMAVYAVLLALAVGVATI
                     LPGGFIPAQDKQYLIAFAQLPSGASLDRTDDVIRRMSDIALHEPGVKSAIAFPGLSIN
                     GFTNSSSAGIVFVTLDDFDKRKSPDLSANAITMHLNQKLASIKDSIVAVFPPPPILGL
                     GTLGGFKLQIEDRGAQGYADLDRVTQAFAARAMQTPELGPTFSNYQVNVPQLDVDLDR
                     VKAKQLGVPVTEVFDTMQVYLGSAYVNDFNRFGRVFQVRAQADAQFRSHPEDILQLKT
                     RNNTGEMVPLSSLVTVKQSFGPEMVVRYNGYTASDINSGPALGYSTNQAQAAAERIAN
                     EVLPRGFKIEWTDLAYQQMLAGNAGVWVFPISVLLVFLVLAALYESLTLPLAVIMIVP
                     MSILAALTGVWLTHGDNNIFTQIGLMVLVGLSAKNAILIVEFARELEMQGSTPLAAAI
                     EACRLRLRPILMTSIAFIMGVVPLVMSTGAGSEMRHAMGIAVFFGMLGVTLFGLFLTP
                     VFYVLLRKLSGSSLHSAARHDAPLVHDTHVPAEH"
     gene            13000..14481
                     /locus_tag="VGC69_14625"
     CDS             13000..14481
                     /locus_tag="VGC69_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0296560.1"
                     /translation="MTSTIQRGSIALALLLMLAGCTVGPDYQRPAVDTPAAYKEAAAA
                     APLPAGEAGSWQEAKPSEDALRGEWWKLFQDPALDALETQAQDANQNLKAAAARLAQA
                     RALLGNAKADRYPELDAGFGPMRERASPASQGFADDGPAFSSTLWRAQGSVSYEVDLF
                     GRVSSEVDAATADAQQSAALLRSVLLALQADVAQGYFQVRELDSELQVYRRTVELRGQ
                     TMQLIQHRYDAGDISELDLARARTELESARADEAGIARSRAAAEHALAVLLGKTPAEF
                     SLPAQPLTRIAVDIPAGLPSNLLERRPDIAAAERTMAGANARIGMAQAAFFPSLSLTG
                     AAGFESSALSNLFHWSSRTFLLGPLVGTALSLPIFDGGRRQAGVDRARAVYEEDVANY
                     RQTVLTAFREVEDNLSSLRLLHGQTAAQDAALAAATRAASLSQTQYREGATSYLDVLD
                     ADRSALQQQRAAAQLDGDRARTTVNLIRALGGGWDPAQQMAVK"
     gene            14562..15125
                     /locus_tag="VGC69_14630"
     CDS             14562..15125
                     /locus_tag="VGC69_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3'-deoxyribonucleotidase"
                     /protein_id="HEY0296561.1"
                     /translation="MIILIDQDGVLADFEHAFIAAWQARHPGIAPVAFEDRKSFNLLE
                     DYPAELRGRAEAIYTAPGFIRDLPPVPGALEAFRELLALGMDVRICTAPLRQYENCVA
                     EKFQWVERHLGRAATERIVMTRDKTLVHGKLLIDDKPLIEGAVSRPAWRHIVYDAPYN
                     RHVADRPRLTWANWRNALAGELYTADN"
     gene            complement(15273..16409)
                     /gene="rodA"
                     /locus_tag="VGC69_14635"
     CDS             complement(15273..16409)
                     /gene="rodA"
                     /locus_tag="VGC69_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY0296562.1"
                     /translation="MKRLGIVLLRVITALDWPLLFILLLFAALGMTVMHSAVGSMDGR
                     FVEQSRNFLVALAAMWAVALIPPNQLMRLAVPFYVLGIVLLLGVHFAGETSKGATRWL
                     NIGFTRIQPSEMLKIAVPLMLAWYFHRHEGAVRIRDFLVAGLLLALPCGLIVLQPDLG
                     TAVLVFGAGFFVIYFAGLSFKLLIPATLVGVLAVGALVYNEDRLCDPSVTWPGLHDYQ
                     KHRVCTLLDPSTDPLGKGFHTIQSMIAVGSGGLYGKGYMQGTQSHLDFIPERTTDFIY
                     AVYSEEFGLYGGVAILVLYSLLIARGLTIAAGGSTQFGRLLAGALTMMMFLYVFVNIG
                     MVTGVLPVVGVPLPFMSYGGTALLTMGVACGVLMSVRRYKIVNA"
     gene            complement(16406..18484)
                     /gene="mrdA"
                     /locus_tag="VGC69_14640"
     CDS             complement(16406..18484)
                     /gene="mrdA"
                     /locus_tag="VGC69_14640"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0296563.1"
                     /translation="MFEFKNTSLQQKQRFRLRALAGAAVVLACFGVLVSRFWVLQVER
                     YQGFSERADRNRIAVVPIPPRRGEVLDRNGEVLARNFRTYTLEVTPSEIDDFDELFDR
                     LSQVVYLSPSDRRKFKRQLNESGRHATVVLRNNLNDTEAAWFASHAFMFPGVELKARW
                     VRQYPLGTSAAHVVGYIGRIADSDLDDLDRAGETGNYRGTDSIGKKGIEKTWESVLHG
                     KTGVQEVEITAGGRPVRTLRRTDPVSGSDIMLSLDMGLQRVAEKAFEGLRGALVAIDP
                     ETGEVLAFVSMPSYDPNLFVDGIDADNWRQLNESPDHPLINRPLYGTYPIGSTYKPFV
                     ALAALELGKRSPTELIPDPGYFEFGGQKFHNAGGAAYGPTDMHKAIVVSSDTYFFSLG
                     PVIGVNPLHDFTKQFGFGQITGIDLEGERTGVLPSTEWKRKAYKDKERQRWYAGESVS
                     LTVGQGYNAFTLLQLGQAVSTLADDGLYRRPHLVHAIRDSRTGEVVPTPSVPDHQIPI
                     KQQYLDIVKSAMADVTRVGTARLAFQGAAYPSAGKTGTAQVYSLRGARYRAGVVDERL
                     RDHALYIGFAPVQHPRIAVALIVENGGWGASAAAPIARKVFDYWLVDKDKLQAAGKPA
                     DAPVPDPMAARAAAVHRPLAQESDNAALAGSVFRREPVPPAEPAAPAPEPLPLPAAPA
                     RAQTGRGETQ"
     gene            complement(18497..18961)
                     /gene="mreD"
                     /locus_tag="VGC69_14645"
     CDS             complement(18497..18961)
                     /gene="mreD"
                     /locus_tag="VGC69_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY0296564.1"
                     /translation="MWGTLIVVWLASMLPWRDWPAAPDIMALVLAFWSAHERRRVGLV
                     TAFVFGILLDVHDTALLGGHALTYVLVAYGAQLLDRRLQRFDLWSQAVHMLPVFLIAP
                     LCTHLVVAWLAGHWSGWGWTLSALITAALWPIVGWVLQLPQRRLDDVESSAV"
     gene            complement(19065..19952)
                     /gene="mreC"
                     /locus_tag="VGC69_14650"
     CDS             complement(19065..19952)
                     /gene="mreC"
                     /locus_tag="VGC69_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY0296565.1"
                     /translation="MQRQSTTPPLFRRGPPAEVRLAVLVVLALALMLADARMRVLEPV
                     RRAVAIALYPFQRVVMAPRDLVEQVDEWVNAANLIRSENEALQRQRIELAQVSTHAQQ
                     VAAENAQLRRLLGITDHTPEPAVVVEVLYEPPSGSGQQRLVFNKGSQAGLAAGMPVID
                     EGGVVGQLVRVTPMTSELAMLTDDRVSIPVQVLRNGLRLIAFGTNTPGEVEVRYLSAN
                     ADVREGDVLITSGVGGLFPAGLQVAKVTSVDRNSASGFAQAVAEPLSHPERYRHFLVL
                     QVDVDQAMKMQESDDDDEE"
     gene            complement(20033..21076)
                     /locus_tag="VGC69_14655"
     CDS             complement(20033..21076)
                     /locus_tag="VGC69_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0296566.1"
                     /translation="MFGFLRSYFSSDMAIDLGTANTLIYVRGKGIVLDEPSVVAIRHE
                     GGPNGKKIIQAVGREAKQMLGRVPGNIEAIRPMKDGVIADFTVTEQMLKQFIRMVHPR
                     NMLAPSPRIIVCVPCGSTQVERRAIRESALGAGASHVYLIEEPMAAAIGAGLAVSDAS
                     GSMVVDIGGGTTEVAVISLGGMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEPTAEL
                     IKKEIGSAFPGSEVREIEVKGRNLAEGVPRSFTVSSNEILESLTDPLNQIVSAVKIAL
                     EQTPPELGADITDKGIALTGGGALLRDLDRLLQEETGLPVLVAEDPLTCVVRGCGEAL
                     EHLEKLGAIFIND"
     gene            21359..21667
                     /gene="gatC"
                     /locus_tag="VGC69_14660"
     CDS             21359..21667
                     /gene="gatC"
                     /locus_tag="VGC69_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY0296567.1"
                     /translation="MALNEQDVARIARLARIELTAEQRGRAQAELNGILHLIERLQAV
                     DTQGVEPLAHPLSAHEDVVLRLRADAVTEPASEARRSELLANAPDTRDGLFLVPKVLE
                     "
     gene            21728..23263
                     /gene="gatA"
                     /locus_tag="VGC69_14665"
     CDS             21728..23263
                     /gene="gatA"
                     /locus_tag="VGC69_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY0296568.1"
                     /translation="MATSALHNEFGDIAGLREALAQRRVGAVELAQSALAAAEAAAHL
                     NAFLHIDADLTLAQARAAEAAIAAGTAGPLAGIPIAHKDVFVTRGWRTTAGSKMLESY
                     VSPFDATVVERLRQAGAVSLGKLNCDEFAMGSSNENSAYGPVHNPWDANAVPGGSSGG
                     SAAAMAAGLAMATTGTDTGGSVRQPASLCGVSGIKPTYGTVSRFGMIAYGSSLDQAGV
                     FAHGSRDLLELLDVMSGFDPQDATSLQACDGAANVPGRVRAAFDAAQAGFDGAGSLPL
                     RGLRIGVPKEFFGAGLAADVAAPVEAALKQFEALGAQRVEISLPRTELSIPSYYVIAP
                     AEASSNLSRYDGVRFGHRAADYADLTEMISRSRAEGFGEEVKRRILTGAYVLSHGYYD
                     AYYLQAQRLRRMIAQDFQLAFADRCDVIMGPVSPTVAWDIGKKRSDPTSEWLMDIYTL
                     SLNLVGLPGMSVPVGFGGERRNRPVGLQVIGNYFDEGRLLAIADRYQQVTDWHKRKPA
                     TQA"
     gene            23331..24788
                     /gene="gatB"
                     /locus_tag="VGC69_14670"
     CDS             23331..24788
                     /gene="gatB"
                     /locus_tag="VGC69_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY0296569.1"
                     /translation="MDWEIVIGLETHTQLSTDSKIFSGSSTRFGAAPNTQANVVDLAL
                     PGSLPVMNRGAAERAIRFGLAVGGAVAPRSVFARKNYFYPDLPKGYQISQYELPVVQG
                     GSVSFFVGEEEKTVNLTRAHLEEDAGKSLHDDFALGNGKPASGIDLNRAGTPLLEIVT
                     EPEMRSAAEAVAYARALHSLVMWLGICDGNMQEGSFRCDANVSVRPRGQKEFGTRAEI
                     KNVNSFRFLERAILFEAKRQIELIEDGGTVVQETRLYDSDRDETRSMRSKEDAHDYRY
                     FPDPDLPPLVIAPEWVEQVRAAMPELPDTLRQRFAADYGLSAYDAAQLVSSRAMAGYF
                     EETAKSLPAGQAKLAANWVMGEVAAQLNREEKDMAGCPVPAAALAALIGRILDGTISN
                     KIAREVFAAMWAGEHGGAPDAIIDARGLKQISDTGAIAAMIDEVLAANPAIVEEYRAG
                     KQKAFNSLVGQIMKAARGKANPQQVNELLKQKLDS"
     gene            24793..25983
                     /locus_tag="VGC69_14675"
     CDS             24793..25983
                     /locus_tag="VGC69_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296570.1"
                     /translation="MSKAYARKALVASTVGYALDGFDLLILGFILAAISKDLGLSSTQ
                     AGLLVTWTLAGAVVGGILFGWLADRLGRVRVLNWTILIFAVFTGLCALARGYDDLLVY
                     RTIAGLGLGGEFGIGMALAAEACRPEQRARMTSFVGLGWQGGVLAAAILTPLLLPHIG
                     WRGMFAVGVLPAVAAFIIRHYIEEPRLFLDARQAPAVNSFKLLVADSATARTSLGVVI
                     LTSVQNFGYYGVMIWMPSYLSNQFGYTLTKSAGWTAVTVIGMALGLWLFGQLADRVGR
                     RPIFLLYQAGAMLSVLAYSQLQSQYALLAGGAVMGLFVNGMIGGYGALIAELYPTRAR
                     ATAQNVLFNIGRAVGGFGPLAVGSLAARYSFQVAIGLLAVIYIADILATLFLIPERKG
                     SALE"
     gene            26019..26696
                     /gene="pyrE"
                     /locus_tag="VGC69_14680"
     CDS             26019..26696
                     /gene="pyrE"
                     /locus_tag="VGC69_14680"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0296571.1"
                     /translation="MPMTSATPTDFVRFALDEGVLRFGSFKVKSGRISPYFFNAGLFN
                     GGRSVGRLAGFYAQALLDSGVAFDMLFGPAYKGIPLATATAVALAGHPGMQGRDVPFA
                     FNRKEAKDHGEGGTLVGAPLKGRVVIIDDVITAGTSVRESVEIIRAAGAEPAAVLIAL
                     DRMERAGADDALSSHSAVQEVAKTYGMPVISIASLKDIMALLEQDPAFAEHRGAVAAY
                     RARYGVL"
     gene            26699..27490
                     /locus_tag="VGC69_14685"
     CDS             26699..27490
                     /locus_tag="VGC69_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0296572.1"
                     /translation="MFRVISANLNGIRSASTKGFFDWMRKQDADVIGVQEIKAQHDHV
                     TGVFDRHDKLAGYFHYAEKKGYSGVGLYTRHEPSAVIRGFDATEFDAEGRWVELRFDT
                     PERKFSVISCYFPSGSSGDERQQAKYRFLDRMYPHLMRLKAERQFILMGDINIAHKEA
                     DLKNWKGNLKNSGFLPEERAWMTKLLGEGGLVDVYRALHPDTTDACYTWWSNRGQAYA
                     KNVGWRIDYQLATDRVAACARASTIYKDAKFSDHAPLTVDYDFSL"
     gene            complement(27529..29106)
                     /locus_tag="VGC69_14690"
     CDS             complement(27529..29106)
                     /locus_tag="VGC69_14690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0296573.1"
                     /translation="MLHFLRSIRRIYLVSWLAVGAVLLLLLLSGSILSSLRLDGYARA
                     LYSGTMVPISQLDAFRAAELDARRQLWRGLALLSDTDSVIQSQREIHLSLVRMSTVWS
                     LYYPSGVSGGDEQVIADNLRDALPRFKAMMEEGLSLLGNRDAAAIANWRNGNEQFLNG
                     FDRLVGQCIASNLREAESLSSQGSAIFRAIIWVGLLLVGLLMFVSGLVVYRLVRDRNE
                     ALSEAHQHLWMANRIFENTQSSVALLDHDGRIVRVNPAFTRLTGYAMSETAGKPLSML
                     NSGRQSEMQYRELEQVLREKGRWSGDLWNRTKNGAIYRESVLIAGIGDHLGGYSNYVV
                     MGLDASERPRVEGRMAELLMRDALTDLPNRLHFNERLAHAVVRAQRNAMRVAVLFLDL
                     DGFRQVNTALGHSLGDEVLRVIARRLERRLRASDGIARLGGDEFAILLEDIHEADDVA
                     GVARKLLTHLDQEIRLNGHHLQVTASIGISLYPDDGLEAGQLLDRADKARYEAKAAGK
                     NRYCFYMPPQDSHVPVE"
     gene            complement(29275..30540)
                     /locus_tag="VGC69_14695"
     CDS             complement(29275..30540)
                     /locus_tag="VGC69_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide transporter"
                     /protein_id="HEY0296574.1"
                     /translation="MTDASPTVPAGNVYLSRRVAPLLALGFASGLPLALTSGTLQAWA
                     TVENVSLQSIGFLTLVGTAYTLKFLWAPLIDRYAPPWLGRRRGWMLLTQLLLALAIMG
                     MSLLSPGSALPDLAALAVLVAVLSATQDIAFDAYSTDVLHSAERGAGAAVKVLGYRLA
                     MIVSGGLALVLADSWLGWGHTYLLMGGLMLACMAATLWAPEPESPAAAPRNLGQAVVE
                     PFREFFSRPGAVGLLLLIVLYKLGDAFAGALSTTFLIRAAGYTATEVGTVNKVLGLVA
                     TIVGALAGGSIMSRWGLYRSLMAFGLLQAVSNLGYWLISVSPKHVWLMAGGVGIENLC
                     GGLGTAAFVGLLMALCRQEFSATQFALLSALSAVGRTYLAGPLTPTLVEHLGWPGFFL
                     GTVAIAVPGLLLLRVHRAQIDQLEKQGQE"
     gene            complement(30569..31183)
                     /gene="metW"
                     /locus_tag="VGC69_14700"
     CDS             complement(30569..31183)
                     /gene="metW"
                     /locus_tag="VGC69_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY0296575.1"
                     /translation="MSPVSVRSGPVVLRPDLARIADWIEPGSRVLDLGCGDGDLLAWL
                     RDNRQVSGAGVEIDDSHVITCVQRGVEVIQQNLEGGLALFDDKQFETVVLSQTLQSMH
                     HTTHILREMTRVARYGIVSFPNFGYWPHGWSILRGRMPVTGQMPYQWYNTPNIHLCTL
                     RDFEDLAAQVGLRILERATFNEAREVRVLPQWRSTLAMYRFSAD"
     gene            complement(31180..32442)
                     /locus_tag="VGC69_14705"
     CDS             complement(31180..32442)
                     /locus_tag="VGC69_14705"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY0296576.1"
                     /translation="MTLIAPHEAGASDHAAPAPAPKSPSRPAPAPGSVGVVSPIFLAF
                     TEPLPLASGQQLDAYELAVETYGTLNAEHSNAVLICHALNASHHVAGVAADDQSQGWW
                     DNMVGPGKPVDTDRFFVIGINNLGSCFGSTGPASLNPATGKPWGAAFPVLTVEDWVQA
                     QARVADHFGIERFAAVMGGSLGGMQALSWAITCPERVAHCIVIASTPRLSAQNIGFNE
                     VARRAIITDPDFNGGDYYAHDTVPGRGLSVARMIGHITYLSDDDMAEKFGRAQREPAE
                     DGAYRYGYDVEFEVESYLRYQGEKFSRYFDANTYLLITRALDYFDPARNAGGDLARAL
                     SPARADFLLVSFSTDWRFPPERSREIVRALLKNGSPVTYAEIDAPHGHDAFLLDDTRY
                     HAVVRGYCERIARELGLPQAAGVSGVAE"
     regulatory      complement(32432..32511)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            32653..32853
                     /locus_tag="VGC69_14710"
     CDS             32653..32853
                     /locus_tag="VGC69_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0296577.1"
                     /translation="MPEILLSDRERDCLHWAALGKTSWETGHILGLSQHTINFHVRNA
                     CVKLGASNRRAAVAMALRLGLL"
     gene            32884..33051
                     /locus_tag="VGC69_14715"
     CDS             32884..33051
                     /locus_tag="VGC69_14715"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0296578.1"
                     /translation="MKTRRVFKTRFFQRWMRKPGLTDATLREAVQEMSAGLVRAVADG
                     TPQEICDGNQG"
     gene            33035..33352
                     /locus_tag="VGC69_14720"
     CDS             33035..33352
                     /locus_tag="VGC69_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEY0296579.1"
                     /translation="MATKAKSRILDTVHETAVDLHRLGFIDKRKMRKYEALCLEPVPE
                     YDAAKIRALRARFHLSQAVLAAALNTSSSTVRKWEAGDKRPSGPSQKLLNLIDRKGLG
                     AVL"
     gene            complement(33612..35762)
                     /locus_tag="VGC69_14725"
     CDS             complement(33612..35762)
                     /locus_tag="VGC69_14725"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY0296580.1"
                     /translation="MEKPREESQILEALAALAASPGYAHAVAHICHRDNVIHIQGKLK
                     PADMDRLFSRERLNRAELTTIIGLMAKKPLDLALQQPDVLEGYVKRTDTLMHELHEAM
                     SFPMFAAMFEAMKAGTEPPDPWRGPGMREPIFYGTESAYAFQYRDLLPEKYGADDEWM
                     LKAKGFTSSQARSIAKTMCVLMDEKGTRLLVGARAAKASPASWLPAFEFAPDEVAFRS
                     GASKDVVEAFFRALLFAGDNAGFKEVGDFNEVAARPLLPTDRGTVLLFSHYAIYEAMY
                     ESPFFWMWDDKPYRPIAAKHRGAFTEQFAARRLVGVFGREHVHTNVNLHRGKDIVGEA
                     DVLVIYGDRLIIVQAKAKKLTIAARKGNDSQLKADFSAAIQDSYDQGWACANEMLAGG
                     CRLVDDNGKHVELPPTIKEVFLFGLVSEHYPALAFQASQSLKFQTTDVIRPPFVMDVF
                     LLDTLTEMLATPLRLLGYVRLRVAVADKLMTGHELTVLGYHLKQNLWLDEKYDCFMLD
                     DSVAQGLDTAMLVRRDNLPGDDTPVGILTKMRGTRYESLIKEIEARADPATLELGFHL
                     LAMDEDACRNVHRVVETITRQAHLDGKRHDITLASSAPPGGVTFHCNPTPSPEAIAVL
                     ETYCTMRKYQQRAAQWFGVSVDPAGKVQFGVTLDYPWEASDEMDRLTADMTPPAKVRD
                     TFPRHSREVRRLKLGPNDPCHCGSGLKYKKCCLN"
     gene            36486..37877
                     /locus_tag="VGC69_14730"
     CDS             36486..37877
                     /locus_tag="VGC69_14730"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEY0296581.1"
                     /translation="MQVREISIADALSLSKQPEGHFYDRKAAQVKGVKLQKIVCAFAN
                     ADGGDVYVGIADDKDEADPTKRWVGSPTMEEFNQLIQSLMEMRPSPPMTMEFLKSTLS
                     SNYVLHIQVDKSQSVHQTTDGTVYERKGAQSLPIKEAERITALGFAKGASSYEDMQVD
                     SADAEDVVDADEIKVFLNEYSPKTDPLDLSINKGLIDRRTFKPKACGLLLFANDPSGV
                     VPKKCSVKIVRYETKEDDPERDHLAFTETVEGPLFPLIKKSVARVTEIMSSISVWTTE
                     GAKSMEYPPETLWEIVVNAVIHRDYSVSDDVQILIFDNRIEILSPGRLPGFVSRDNIL
                     DVRYARNPKIVGMLSKYKDAPNKDIGEGLNTAFQKMKEWKMRNPEIIEEDNYIRVVIP
                     HASLATPQEAIMEFLSNNQTITNKQARDITGIKSENAVKSEFYKLRDTGRIEMIPELK
                     GNKAAWRLVAATS"
     gene            38190..39182
                     /locus_tag="VGC69_14735"
     CDS             38190..39182
                     /locus_tag="VGC69_14735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0296582.1"
                     /translation="MAQTRISYRKLGHSDLNVSAIGLGCMSLSGVYGAADDAVSEALI
                     QHALDMGVNHLDTSDMYGWGHSEQVVGRAIKGRRDKVMLASKFGQIKQEGAGNGVNGR
                     PEYVRQACEDSLKRLGVDVIDLYYQHRVDPDVPIEDTVGAMADLVRQGKVRFLGLSEA
                     HVDTIRKAHGVHPITALQSEFSILYRAEAEETRRVTKELGISFVAYAPLGRGLLTGEI
                     KTLADVDGRRASHPRFQPEHFEHNRKLAEPIEVLAKEKGCTPSQIVLAWLLAQGEDVV
                     TIPGTRKTARLDENVGALDVVLSQAEIDGLTAAIPADAVSGMRYPAASMARVNL"
     gene            39292..40191
                     /locus_tag="VGC69_14740"
     CDS             39292..40191
                     /locus_tag="VGC69_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY0296583.1"
                     /translation="MTTISENPTIALVGPGAIGTTVAAALHEAGRTPTICGRSAHERL
                     ELRVDGERIFVPGPVQIDPAEIKNPFDLVFVAVKSTQVEAVAPWLSALCSARTVVCVL
                     QNGVEQKALFAPHVPGSPVLPSVVWFPAQREPDASVWLRGKARITLPDTPEASLVLAA
                     LRGTRCAVDVAADFTSVAWRKLLQNAVAGLMVLAGRRAGMFSRDDITRLSLAYLQECL
                     EVARAEGASLGDGVPQEIIDGFHRSPADLGTSILADREAGRPLEWDSRNGVVQRRGRS
                     RGIPTPISDLVVPLLAAASDGPG"
     gene            complement(40331..41224)
                     /locus_tag="VGC69_14745"
     CDS             complement(40331..41224)
                     /locus_tag="VGC69_14745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296584.1"
                     /translation="MNPDPKFRQMRAFVTAMESTSFTLAAQKMEISQPSFSALIRDLE
                     SNLQAKLFERTTRRCVPTDAAQGLYEQIKSPLREIELAYRNARDVSNGVGGRLRLAAL
                     PSMASGAITRKLAEFQRAHPDVTITLVERRHDQQREAVLKGEVEFGICTLSGPHPELS
                     FQSLYTDRLMIVVPAKHPLVGSKPNWRTLNDWPLVLVSSGQAEDAMRAQGVSVIPTCD
                     VEQVATALAMVRQGMGITVLSSSIIPDLAIEGLACLPIYGPRVFRSMGVIRRRHVRLS
                     RIAQTFVRMLRDLSPPQFEND"
     gene            41320..42330
                     /locus_tag="VGC69_14750"
     CDS             41320..42330
                     /locus_tag="VGC69_14750"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0296585.1"
                     /translation="MMMLASGPSVWRGVEMAQDPSWVWQLPASGVNTIERALESVMRR
                     NIQVPEMRKEDFALPGLEENFHAIAGEVDTGRGFQVVRGVPVAGYSEDELHRLTWGLG
                     LHFGVPVEQTKDADWLIDVRDEGGSYDRNSRGYHSASKLEFHTDGASIVGLLCVHKAE
                     EGGLSVLVSAGAVHNELARTHPEHLIPLYDGFPVDRRGAQPPGEPRVSPWYLPVFSVT
                     RGQLNCVYSRGACIWGREHLGQPCSEAEIAALDAFEAIASDPGFRLDMDLQPGDLQLV
                     NNFTVLHSRTAFYDNPASPQRRHLVRLWLDVEGSDHAAINKLHLYTNKPLPSLVAVRP
                     RT"
     gene            42374..43372
                     /locus_tag="VGC69_14755"
     CDS             42374..43372
                     /locus_tag="VGC69_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296586.1"
                     /translation="MVTKLTKALRALASAVAMAAVAPAALAQDAGNWPAHQPVKIVVP
                     FPPGGAADINARLLAAKLSEMNSANFYVENHAGANGIIGLDFVAKSAPDGYTLLSSNL
                     SSHGTGPAVYKTLPYDPIKDFIPVVQVTATPMLLVESPKLPYRSLQEVIQAAKANPGK
                     ITIASAGNGSSTHLAAEMLNEMAGIKLLHVPYKGDTPAMVDVASGVCDLLLVSTEASE
                     PFVKSGKLRVLAVGSLKRLPTLPDVPTLSEAGVPGYVGSTWSGLLAPAGTPAAIVDKL
                     NRQVNQIAQTPDFRQRVESAGSQVIGGTPATFGTLIQSEIAKWTRVAHDNNIVLNP"
     gene            complement(43388..44893)
                     /locus_tag="VGC69_14760"
     CDS             complement(43388..44893)
                     /locus_tag="VGC69_14760"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEY0296587.1"
                     /translation="MNTATQATQDVGTTAGALRGRIENGIAVFRGIPYAAPPVGALRF
                     EAPRPPAPWQGVRDATADGPIAPQGRSRLAHVMGEFQRPQSEDCLTLNVWTPGIGKGA
                     RPVIVWLHGGAYSSGAGSLPWYAGDRMAANGDVVVVSVNYRLGALGFLYLPGVSPGNL
                     GLLDQVQALRWVRENIGNFGGDPDNVTLVGQSVGGNTIAAMLTMPTAKGLFQRAILQS
                     PGMSRPTRPVAEAAAQGATYMDLLGLARDDIAGLKQVPVDRLLKAQGELAKSLQSFAT
                     MMLPFTLVQDGNAIPEDITGCLRAGRGAPVDIMLGTLREERAAFFSTDPAIAAADIQA
                     VKKVFEKLCGPDYEAYYDEFRRLRAKPTAASMLGDLLTDYVFRIGTLQTAEMLADHGR
                     PAYVYQFDWQSPAGFQACHCLEIPFVFHNIENWPDAPMLKGVDPAEFDGLAHAMQQAW
                     IAFARTGNPNHDGLPRWAPYDRRHRTTMRFDTVLGPVNDLAGLSSRLPWPA"
BASE COUNT         8006 a        15038 c        14502 g         7437 t
ORIGIN      
        1 tcttggcctt ggcaacccat tcgtcgaaac cttgctgcgt cgtggcgatg gccgagaagc
       61 gcatgcccga gaaaccgccg ccgctgtagt tggccgagag gccggcgtag gtgccggctt
      121 cgcgcgcgat caggctcagc cgggtctgca tgccgctcat cgagtagatc tggctgccca
      181 gctgcgggat gaagaaggag ttcatcaccg tggccgacgt gatgcggaag ttcaccggcg
      241 tatcgaccgg gaacgcgatc tggttcaccg tggcgatgtt gtagtcggga tagatgaaga
      301 gccacttcca gtccatcgac accacatcga tattgatggg cttgacgtcg gacttgagct
      361 ccttgtaggg atcgagctcg tgcgtgctcg tccaggtgat ggtggccagg atggcgatga
      421 tgatgcaggg gatcgtccac accacggcct cgatggcggt ggagtgcgac cagcgcggcg
      481 cataggtggc gcgggtgttg gtggaacgat agcgccatgc gaaggcgatg gtcatgatga
      541 tcacggggat caccacgatc agcatcaggc ccagggcgac gagcagcagg tgtttttcct
      601 gcatgccgat atcccctttg ggatcgagga cttcgacttt acagccgctg agcagcacga
      661 gggcgccgac agcaagtgtg cgcgctatgg ttgcaagaga cgggattttc actgactacc
      721 ttcgtgggtg aagagccgcg attgcagcaa aaccgggatg ataccgctcg gacgtggcag
      781 agacacgtga catggtgtcg cacctacgcc acattcgcac catattggtg cagatgcggc
      841 gcctcagccc tgccgcccct gggagcctct tgcccggggg tgcgacaaaa tgtcgctccc
      901 ccatgttacc aatttgccgc cggccgggat cagtcgtcgg ccgacgggtc ccgctcgggc
      961 ccgggcttct tggccagctt gcgctgcagc gagcgccggt gcatgcccag cagccgggcc
     1021 gcggccgaca cattgccgcc ggtctcggac aaggcctggt ggatgtgctc ccactccagg
     1081 cgatgcaggg gaatcatggc ctcttcgatg accacttccc gcgcttgcgc caggcccagg
     1141 gtgcgcagga tcatgggcac ggtggcaggc ttgggcaggt agtcgtcggc gccccgcttg
     1201 atggcgttga ccgccgtggc cacgctggca tagccggtga cgagcaatat gcgcatgtcg
     1261 gcccgcagcg cgcgcagcgg cgcgatgagc gccaggccgg agtcttcgcc caggcgcagg
     1321 tccaccaggg cgaaggccgg ccgcacggac tcggccaggc gcaaggcctc ggccgcgctg
     1381 caggccagat gggtttccag gccatggcgc gacaggccgc gctgcagggt gcgcaggtag
     1441 agctcgtcgt cgtcgatcag caggccgggg ccgggcaaag tcacgaggaa attccagatt
     1501 gcttgtgcaa gggcaggacg aaacggatgc gcgtgccgcc gcccggcgcg ccggccagcg
     1561 tcatttcgcc gcccagctgc tcgaccgtgg cgtgcgacag cgccagcccc acgcccaggc
     1621 cctgcggctt gccgctgtgg aacaggcccg aggccggcag cagcggccgc tccggatcga
     1681 tgccggcgcc gtggtcgcgc acttcgccgc gcaggatccc atcctgatac gacaagtgta
     1741 attccacgcg caggtccccg gcctcctcgg cggcgtcggc ggcattgttg agcaaagcct
     1801 gcagcaggtg cgcgatggcc ggttcgaccc gcaggctggc cggcagttcg ccggcgcggt
     1861 gcaattcgat ggcgggccgc acgagggtcc attggccgac cacccgcacc aggtcgacct
     1921 ggtcgggcac ggccaggttg cggatgcgtt cgcggcacag ggccagcagt tcgatcagcg
     1981 tcgagatgtc gctgcgcatc tgcgcgccgg gcgcctgccc gccctgcgcc tgatcggcga
     2041 tctcgtcggc cagcagcgtc atggtggcca gcggcgtgtt gagctcgtgc gccatggcgg
     2101 cggcgtgcgt ggccagcgcg acgatgccct cgttgcgcgt gaagcgctcg cgcaaggccg
     2161 ccagctcccg ctcgcggctg cgcaggccgg cggccaggcg tgtcaggaag gagcacacca
     2221 ccgccaccga gatcaggaag gtcgcggcca tgccccacag gatcagggtg tgctcgtcgt
     2281 gggggctgac cagcggctgc ccgaagacgg ccgccacgct gtagccggcc acgctgacca
     2341 gcgccgccag cagcgcccag ctgcgcggca gggccagcgc gcccagcgcc gtcagcacca
     2401 ggaacatcat gccgaagggg ttgccgatgc cgccgctcca ggccaccatc caggtcagca
     2461 ccagcatgtc gaccatcagg tgcatgaagg ccacgcccgg cgccagttcc tcgctgcgct
     2521 gcggcagcac ggtcgccacc aggttgaaga gcaccagcgc cgccacgccg ccccacaggg
     2581 gcaggagcgg cagggagacg tccagcgggc cgctggcgac caggatggcg acggcctgcc
     2641 cggcgatggc gacccagcgc aggacgcaca aggtgcgcag gaatgaatgg gcgggacggc
     2701 cggtcatgct cgatgggcgg ggggactgat aggagcctag tatgcgctgg ggacttaccc
     2761 ggattgtcac atcaatgaaa acaacctaca atatgctttc atattccagc atttttgctt
     2821 aatccgcatc gatttttcgt cgaaaaatcg gctttcactt tcagccaccc tggctgtgcc
     2881 gcgggcctcc ctggcccgct cccttgaaag ccgcaggatc cgaaggctgg ctcgcccgcc
     2941 tttgcgctgc ccgatgcagc ctgcctgcga tgcacgccgc gtccccccaa gtcccatccc
     3001 cggcgcggca ttccggattc cgcaccatcg gcatccggat cacacaggag gacgaaccca
     3061 tgttcctggc caccgatctg gacggcactt tccttgccgg cgacccggaa gaccgactgc
     3121 gtctgtacca gaccatcgtg tcccatccgg aaatccagct ggtctacgtc agcggccgca
     3181 gcctcgagca catcctgccg ctgctcgacg acccgtccct gcccaatccc gactacatca
     3241 tcgccgacgt cggcgccacc atcgtccacg gcgacaccct gcagccggtg cagcccctgc
     3301 agacccgcct cgaaacccgc tggccgggcg aacaggcggt caccgaggcc ctgtcggcct
     3361 ttccctggat caagcgccag gacgtgccgc aatcgcgccg ctgctcctac tactgcgagc
     3421 cgtgccgcag cgatgatccc gacctgcgcc aggcggtcga ggcgctgggc tgcgacctgc
     3481 tctattcggc cgacaaatac ctggacgtcc tgccgcgcgg cgtcgacaag ggcaacacgc
     3541 tgcgcgccct ggtcgaacac ctggaactgg atccggacgc gatcctcgtg gccggcgaca
     3601 cgctcaacga cgccagcatg taccgggaag gattcatcgg cgtttgcgtg ggcgactccg
     3661 agcccgccct gctggcgcag accggcaaca gcgcccgcgt cctgcacgcc gcgcgccgcg
     3721 gctgcggcgg catactcgag gccttcgcct atttcggctt cctgggcgaa cagggcgtgg
     3781 aggccgaact gccgcaggcc ctgccgcccg gcggatcgga cctggtcatc gtctatcacc
     3841 gcctgccgta tgaagaaaag aaggtgaacg gcgcgatcca gcgccgccgc cctacctcgc
     3901 ccaacggcat catccccacc ctgctgggct ttttcgacga cggccagccc ggttcgtggg
     3961 tggcctgggc cgagcacgag gacacggacg gcgagttcga ggtgcacacg gcggtggaca
     4021 cgcgccgcta tccccggctg accgccgccc gggtggccct gtccaaggcc gacgtggacg
     4081 tgttctaccg gcgcttttcc aaggaggcct tctggcccac cctgcatacc ttctgggagc
     4141 gcgcgcgctt caacgaggat gactggcagg tcttcctgcg cgtgaaccgc atgttcgccg
     4201 aacgcaccgc ggcggaggcg gcgcacggcg ccgtggtctg gctgcacgac tacaacctgt
     4261 ggatggtgcc ggcctacctg cgcgagctgc ggcccgacct gcgcatcgcg ttcttccacc
     4321 acacctactt tccgtcggcc gacgtgttca atgtgctgcc ctggcggcgc agcatcgtgg
     4381 gcagcctgct gcagtgcgac tacatcggct tccacatccc gcgccaggtc gagaatttcg
     4441 tcgacgtcgc cgcgggcgtc atgccgatcc gccgcgccca gcggcagaat tgcgcgccgc
     4501 gcttcctgac ctacggctgc gcggtcggcc tggaatcggt cacgacggtc atcgagaccg
     4561 aaacccgcac ggtgcggctg ggcgcgcatc cggtcggcct ggacgtcggg cgcgtgcgcc
     4621 gcacgctggc gctggcctcg gtgcaggaga acatgcgcaa gctgcgcgcc gagctgagcc
     4681 gccagcgcgt gatcctctcg atcgaacgcc tggactacac caaaggcacg ctggagaaac
     4741 tctgcgcctt cgaacgcatg ctcgacgaga acccggagct gctcggcaag gtgtcgctgg
     4801 tctgcgtctg cgtgccggcc gcgcgcgaga tgacgatcta cgacacgctg caggtacaga
     4861 tagagcaggc ggtcggccgc atcaacgggc gctactcgca agtgggctgg atgccgctgc
     4921 acttctattt ccggcccctg cctttcgaag aggtcatcgc ctggtatgcc atggccgacg
     4981 tcatgtggat cacgccgctg cgcgacggcc tgaacctggt ctccaaggaa tacgtggccg
     5041 tccagggcct gctcaacggc cagggcgtgc tggtgctgtc ggaattcgcc ggcgccgccg
     5101 ccgagttgaa gggcgccctg ctcaccaacc cgcacgacca ggtcgacctg gtgcgcgcct
     5161 gctataccgc gctgaacctg ccggccgccg aggcgcgggc gcgcctgcgc gagttgttcg
     5221 agatcgtctc gacccacgac gtgcggcgct ggggccagga gttcctggcc gcggtcaacg
     5281 agcagccgga ggcggcgccg gccggctgac gccggcccgc ttcgtcaagg ccttgcggcc
     5341 aggggaacgc ccggccgcga ggccgagcgc gaacccggca gcggctggat gcggccggga
     5401 taataggtgt gcaccagcga gaacttcacc gcatcgccgg cgttgcgctg cgccgcgtgc
     5461 aaggtgcggc aatggaagaa caccacgtcg ccgggctcga gttcgggaca gaccgcctgc
     5521 gcgatccagc gctggttgcc gggcgggtcg ggcaggaaga attttttctc gtcgaattgc
     5581 gcggacgaaa aatcggccgc atgcgaaccc ggcaggaaga acaagccgcc gttgtcggcg
     5641 gtttccggcc ccagggccag ccagcacgac atcagctcgg gcgcgctgaa cgaccagtag
     5701 cggatgtcct ggtgccagcc cgtcaggctg ccgtagctgg gatgcttggt catcacgcag
     5761 ttgtgatgca cggtggacag caccggcgcc tcgccgaaat aggccgccag ccactgcccg
     5821 atgcgcggcg aggtggccca ccgcgcgaac agcggatcgc gcccgtaggc atcgagcagc
     5881 cgccgcacgg tgtgcccgcc ggccgcctgg cgcgaagccg gcgcccccgg atattgcagg
     5941 tcggcctcgt attcgagcgg cgcgatcgcc tcgtcgagat ggcgctgcgc cagggtgcgc
     6001 agggccgcca cggtcgcggc gtcggcaaag ccccgcgcca ccacgaaacc ccgttcgcgc
     6061 agctctgcaa tctggctggc ttgcgtcatg gccggccttc gccgcccggc ggcctgggta
     6121 gttcggcatc gcgctcttcg gccacggtgc gcacccattc gcgccacacc gacaccagga
     6181 gcgccatcag caccggcccg acgaacagcc ccacgatgcc cagcgtcttc aggccgccca
     6241 ccaggccgaa gaaggtcggc aggaaaggca gcttgaccgg tccgcccacc aggcgcggcc
     6301 gcagggtctt gtcgacgatg aagagctcga ccgcgcccca gatgagcagg ccgatgccgg
     6361 ccgcgatgtg gttcgcgccg atgaggtaca gcgacaccag cgtgaacgcc gtcggcgcgc
     6421 cgccgggcac cagcgccatg aagccggtga tcacggccag cagcacgtgc gcgggcacgc
     6481 cggctatcca ataggccgcg cccagcacca cgccctcgcc tatggcgatc aggctcatgc
     6541 ccgtgacggt ggcgccgatg gtggccggta ccatgcgcga gaagcgctgc cagcgcgccg
     6601 gcaggatgcg ttcgcccagc aggtcgatct ggcccaggag gcgctggccg tccttgtaga
     6661 tgaagaacag cgtgagcagc atgaagagca ccgccagcag cagctggacc gcgttgcccg
     6721 cggcgcccag caccatgcgg tagatattgc cgatgtgctc gccgctgatg agctgcacga
     6781 gatcgcccag cgcatgcggc tcgcccaggt aggtgcgcca gtaggtggcc agcctgtcgc
     6841 ccaccagcgg gatggtgggc atccaggacg gcaccgcggc gccgtcgcga ttggccgcca
     6901 gcatccaggc gaaaagatgg ctggcctcgc ggaaggcgta gatcgacgcc agcgacaagg
     6961 gcaccaccac caccagcagc accagcagca gcgcgaccgt ggcgccgacg gtcgtgttct
     7021 gccggcagga caccagcagg cgccggtaga gcggccaggt ggccaggccg atgatcagcg
     7081 ccgccagcgc cggcaccagg aagccgctca ggaaatacac ccccgccaac agcaccagca
     7141 acaaaagcca gcgggcaatc aatgaagcgg gcaagacagg cgacatggag tagacggcag
     7201 gaaaccggcg gaggaattcc gcccaagctc agaaatatac gagttttggg ccgaccctgc
     7261 ctgccggccg tccgcgcggg cccggccagg cggctagacc aggaccccgg agggatcgat
     7321 gggcacgtgc cggcgcgtgg cttcatgggc ctgcgggctg atccttgccc cttcggccat
     7381 gagccgcttg cactcgagca tggagggatc gttctcgtcg cggccgctca gcagcgggca
     7441 accggcgaag agctcggcca cccagtcgga gaacgcgcgc accttgggcg aaagctggcg
     7501 gctctgcagg tagagcagcg agatcggcat cgccgcgggc cggtattcgg gcagcacttc
     7561 gaccagctcg cccttgtaca tgtgcgccag cgccatgaag cgcgccgtct ggatcatgcc
     7621 gaagccctgc acgccgcagg ccaggtgggc cgaccactcg ttgaccgaca cggtgccggg
     7681 aagctcgacc tggcgcggct gcccgtcgac catgaactgc cagccgtggt tgcggcccgg
     7741 tcccaggaag taatgcacgg cttgatgccg cttcaagtcg tcgagcgttt tcggcacgcc
     7801 gtagcgctcg aggtaggcag gcgccgcgca ggtgatgagt tcgacggtgc cgatgcggcg
     7861 ggccaccagg ctggaatcct cgagatcgcc ggcgcggatg acgcagtcca cggcgtcctg
     7921 caccaggtcg atcttgcggt cggacaggcc gatggccagc tccacgtccg ggtagcgggt
     7981 atggaaatca cacagcgcgg gaatgagcac catcgtgccg atgctgggct tgacgtcgat
     8041 gcgcaaacgc ccttgagggt gcgccgacac gtcttgcaga taggcctcgg tttcctcgac
     8101 ctcggccagg atgcgcacgc tgtgcttgta gtaggcggcg ccgtcgggcg tgaggctcag
     8161 gcggcgcgtg gtgcggttga gcaggcgcac gcccagatgc ttttcgaggt tctggatgat
     8221 ggtggtgacg gtggtccgcg gcagcgacag cgcgtcggcg gcgcgggtga agctgttggc
     8281 ttcgaccacc cgggagaaga cctgcatggc ttggaagcga tccatggcgt ttcctgagaa
     8341 gagcgggatg gccgacagca taaaacaaaa ccggccagat tgttagccac tgccgaccag
     8401 tgttgccgga aatagactgt ttattcgggt cgacaaaaca ttcagaatac ggtcaaccca
     8461 gaggaaccag cctcgccgcc ctgcggcaaa ggcctgcaca caggcctcgc ggcccgcgcc
     8521 atgcgcgagc cgctcctatc ccggctaggt ctcccgtccc cctattttac ttcccaagga
     8581 aacgttcatg acggtttctc gaccccgtgc ttttcgcctt ccggcttttc gtccccgctt
     8641 cccggccctg acgctggcca tcctgctgac cgcgacaggc ggctatgccg tcttcggccc
     8701 tgccgcgcaa acgcccgccc tggcccagcc ggcgccgccg gccggcgtgc cggtcgacgt
     8761 ggcgccggcc atcggccgct ccatcgtcga ctggcagcgc tattcgggcc ggctcgaagc
     8821 catcgaccgc gtcgatatcc gccccctggt gccgggcacg ctgacccagg tgcacttcaa
     8881 ggacggcgcc ctggtcaaga agggcgatcc gctcttcacc atcgacccgc gcccctatga
     8941 ggccgaagtc gagcgcgccg aggcccgcgc ggcctacacc gtcgccgacc tgtcccgcgc
     9001 caagcgcctg ctggccgaga acgccatcgc ccggcgcgac tacgaagaaa aggaaaacgc
     9061 cgcccgcgac gccgacgcca gcctcaagac ggcccgcatc aacctgggct acacgcgcat
     9121 cgtggcgccg gtggacggcc gcgtctcgcg cgccgaggtc acggtgggca atgtggtgtc
     9181 ggccggcgcc tcctcggccg tgctgaccac ggtggtgtcg gtggcccgca tgtatgccgc
     9241 cttcgacgtg gacgagcaga ccttcctgcg ctacgtgaat ccgggccgcg ccaagggcac
     9301 gccggtgccg gtgcaaatgg gcctggccga cgaagacggc tactcgcgcg aaggcgtggt
     9361 ggagtcggtg gacaaccggc tcgaccccgc ctcgggcacg atccgcgtgc gcgcggtgtt
     9421 cgacaacaag gacggcctga tgctgccggg cctgtacgcc cgcgtccggc tgggcggcag
     9481 cacgccgcac gacgccgtgc tggtcgacga aaaagccatc ggcaccgacc agaacaagcg
     9541 ctacgtgctg gtggtggacg acgccaacca cgccaattac cgcgaagtgc agctgggcac
     9601 cagcgtcggc ccgctgcgcg tggtgacgca gggcctgaag accggcgagc gcatcgtcgt
     9661 caacggcctg cagcgcctac gccccggcga tgtcgtggcg cccagcgccg tgtccatgga
     9721 cgaccggacg tccgccatca agaccgcttc cgccagcctg aagtaaacga gaacgtcagc
     9781 gcgaacccct actcatgaat atctccaagt ttttcatcga ccggcccatt ttcgcgggcg
     9841 tgctgtcggt gctggtgctg ctgggcggcc tcctggccat gttccagctg cccatctccg
     9901 aatacccgga agtcgtgccg ccctcggtgg tggtgcgggc ccagtacccc ggcgccaacc
     9961 ccaaggtcat cgccgagacc gtggcctcgc cgctggagca atccgtcaac ggcgtcgagg
    10021 acatgctcta catgcagtcg caggccaata gcgacggctc cttcgccctg acggtgttct
    10081 tccgcctggg caccgatccg gacaaggccc agcaactggt gcagaaccgc gtctcgcagg
    10141 ccctgccgcg cctgcccgac gacgtgcagc gcctgggcgt gaccaccgtc aagagctcgc
    10201 ccaccctgac catggtggtg cacctgatct cgcccgacaa ccgctatgac gcgacctatc
    10261 tgcgcaacta cgcggtgctc aacgtcaagg accgcctggc ccgcctcaac ggcgtgggcg
    10321 aagtgcaggt ctggggcgcg ggcgactacg ccatgcgcgt ctggctcgac cccaacaagg
    10381 tcgcccagcg cgacctgacg gccaccgagg tcgtcaatgc catccgcgag cagaacgtcc
    10441 aggtggccgc aggcatcatc ggcgcctcgc ccacggccag cgacgtgccc atgcagttct
    10501 cggtgaacgc gcgcggccgg ctgcagaccg aagaggagtt cggccagatc atcctcaaga
    10561 gctcgcccga cggcgcggtg acgcacctgt ccgacgtggc ccgcatccag ctcgacgcgc
    10621 aggagtacgc gctgcgctcg ctgctggaca acaagcaggc ggtggccatg gccatcatgc
    10681 aggcgccggg ctcgaacgcg ctggacgtgt cgcaccaggt gcgcgaggcc atggccgagc
    10741 tgtcccagga cttcccgccc tcggtgcaat accgcatcga gtacgacccc acgcaattcg
    10801 tgagcgcgag catcgaggcc gtggtgcaca ccctgctgga agccctcgtg ctggtggtgc
    10861 tggtggtgat cgtgttcctg cagacctggc gcgcctcgat catcccgctg ctggccgtgc
    10921 ccgtgtccat catcggcacc ttctcgctgc tgttgctgtt cggctattcc atcaatgcgc
    10981 tgtcgctgtt cggcatggtg ctggccatcg gcatcgtggt ggacgacgcc atcgtggtag
    11041 tcgagaacgt cgagcgcaac atcgaggccg gcctgtcgcc gcgcgaggcc acctatcgcg
    11101 ccatgcgcga ggtcagcggc cccatcgtcg ccatcgcgct gacgctgtgc gcggtgttcg
    11161 tgccgctggc cttcatgacc ggcctgtcgg gccagttcta caagcagttc gccgtgacca
    11221 tcgccatctc gacggtgatc tcggccttca actcgctgac gctgtcgccg gccctgtcgg
    11281 ccctgctgct caagccgcac gacgagcaga ccgactggct gtcgcgcgcc atgaacaagg
    11341 tgctcggccc cttcttccgc ggcttcaacc ggctgttcgg ccgcgcctcc aaccgctacg
    11401 ccgccggcgt gagcggggtg ctgcgccgca agggttcgtc catggcggta tacgccgtgc
    11461 tgctggcgct ggccgtgggc gtggccacca tcctgcccgg cggcttcatt cccgcgcagg
    11521 acaagcagta cctgatcgcc ttcgcgcagc tgcccagcgg cgcctcgctc gaccgcaccg
    11581 acgacgtgat ccggcgcatg agcgacatcg ccctgcacga gcccggcgtc aagagcgcca
    11641 tcgccttccc cggcctgtcg atcaacggct tcaccaacag ctcgagcgcc ggcatcgtgt
    11701 tcgtgacgct ggacgacttc gacaagcgca agagccccga cctgtcggcc aacgccatca
    11761 ccatgcactt gaaccagaag ctcgcgtcca tcaaggattc gatcgtggcc gtcttcccgc
    11821 ctccgcccat cctgggcctg ggcacgctgg gcggattcaa gctgcagatc gaggaccgcg
    11881 gcgcgcaagg ctatgccgac ctggaccgcg tcacgcaggc cttcgccgcg cgcgccatgc
    11941 agacgcccga gctcggaccc accttctcga actaccaggt caacgtgccg cagctcgacg
    12001 tcgacctgga ccgcgtcaag gccaagcagc tgggcgtgcc ggtgaccgag gtgttcgaca
    12061 ccatgcaggt ctacctgggc tcggcctatg tgaacgactt caaccgcttc ggccgcgtgt
    12121 tccaggtgcg ggcccaggcc gacgcccagt tccgttcgca ccccgaggac atcctgcagc
    12181 tcaagacccg caacaacacg ggcgagatgg tgccgctgtc ctcgctggtg acggtcaagc
    12241 agagcttcgg cccggaaatg gtggtgcgct acaacggcta caccgcctcc gacatcaaca
    12301 gcggtccggc gctcggctac tcgaccaacc aggcccaggc ggcggccgag cgcatcgcca
    12361 acgaggtgct gccgcgcggc ttcaagatcg aatggaccga cctggcctac cagcagatgc
    12421 tggccggcaa tgccggggtg tgggtgttcc ccatcagcgt gctgctggtc ttcctggtgc
    12481 tggccgcgct gtatgaaagc ctgaccctgc cgctggcggt gatcatgatc gtgcccatga
    12541 gcatcctggc ggcgctcacc ggcgtgtggc tcacccatgg cgacaacaac atcttcaccc
    12601 agatcggcct gatggtgctg gtggggctgt cggccaagaa cgcgatcctg atcgtggagt
    12661 tcgcccgcga gctggaaatg cagggcagca cgccgctcgc ggccgccatc gaggcctgcc
    12721 gcctgcgcct gcgtcccatc ctgatgacgt ccatcgcttt catcatgggc gtggtgccgc
    12781 tggtgatgtc caccggcgcc ggctcggaaa tgcgccatgc catgggcatc gccgtgttct
    12841 tcggcatgct gggcgtgacg ctgttcgggc tgttcctgac gccggtgttc tacgtgctgc
    12901 tgcgcaagct gagcggcagt tcgctgcact cggcggcgcg ccacgacgcg cccctcgtcc
    12961 acgacaccca tgtccctgcc gaacattgag gaatttttca tgacaagcac gattcaacgc
    13021 ggatccatcg cgctggcctt gctgctgatg ctggcaggct gcacggtggg ccccgactac
    13081 cagcggccgg cggtcgacac gcccgccgcc tacaaggaag cggcggccgc cgcgccactg
    13141 cccgccggcg aagccggcag ctggcaggaa gccaagccct ccgaagacgc tctgcgcggc
    13201 gaatggtgga agctgttcca ggaccccgcc ctcgacgcgc tggaaaccca ggcgcaggac
    13261 gccaaccaga acctgaaggc cgccgccgcg cggctggccc aggcgcgcgc gctgctgggc
    13321 aacgccaagg ccgaccgcta cccggaactc gacgcgggct tcggtcccat gcgcgaacgc
    13381 gcctcgcccg cttcgcaagg ctttgcggac gacgggccgg ctttcagcag cacgctgtgg
    13441 cgcgcccagg gctcggtgtc gtacgaggtc gacctgttcg gccgcgtttc gtccgaagtc
    13501 gatgccgcca ccgccgacgc ccagcaaagc gccgcgctgc tccgctcggt gctgctggcc
    13561 ctgcaggccg acgtggcgca aggctacttc caggtgcgcg aactcgattc cgaactgcag
    13621 gtctaccgcc gcacggtcga gctgcgcggc cagaccatgc agctgatcca gcatcgctac
    13681 gacgccggcg acatcagcga gctggacctg gcgcgcgcgc gcaccgagct ggaatcggcg
    13741 cgcgccgacg aggccggcat cgcccgcagc cgcgccgcgg ccgagcacgc cctggccgtg
    13801 ctgctgggca agacgccggc cgagttcagc ctgccggccc agccgctcac ccgcatcgcg
    13861 gtcgacatcc ccgccgggct gccgtcgaac ctgctggagc gccgccccga catcgcggcc
    13921 gccgaacgca ccatggccgg cgccaacgcg cgcatcggca tggcccaggc ggccttcttc
    13981 cccagcctgt ccctgacggg cgccgcgggc ttcgagtctt ccgccctgag caacctgttc
    14041 cattggtcca gccggacttt cctgctcggc ccgctggtgg gcacggccct gtcgctgccc
    14101 atcttcgacg gcggccggcg gcaggccggc gtggatcgcg cgcgcgccgt ctacgaggaa
    14161 gacgtggcca actaccggca gaccgtgctg acggccttcc gcgaagtcga ggacaacctg
    14221 tcgagcctgc ggctgctgca cggccagacc gccgcgcagg atgccgcgct ggctgccgcg
    14281 acgcgggcgg cgagcctgtc gcagacgcag taccgcgaag gcgcgaccag ctacctggac
    14341 gtactcgatg ccgaccgcag cgcgctgcag cagcaacgcg ccgcggccca gctcgacggc
    14401 gaccgggccc gcaccacggt aaacctgatc cgcgcgctgg gcggcggctg ggacccggcg
    14461 cagcagatgg cggtgaagta gaccggcatt gccatgcacg gccggacggc ctgcccgtgc
    14521 atggcacaat gggcgctccc catcactgcc gcactgcaac catgatcatc ctgatcgacc
    14581 aggacggcgt gctcgcggat ttcgagcatg ccttcatcgc cgcctggcag gcgcgccatc
    14641 ccggcatcgc gcccgtggca ttcgaggatc gcaagtcctt caacctgctc gaggactatc
    14701 cggccgagct gcgcggccgg gccgaggcca tctacaccgc ccccggcttc atccgcgacc
    14761 tgccgcccgt cccgggcgcc ctcgaggcct ttcgcgaact gctggcactg ggcatggacg
    14821 tgcgcatctg caccgcgccc ctgcgccagt acgaaaactg cgtggccgag aagttccaat
    14881 gggtcgaacg ccacctgggc cgcgccgcca ccgagcgcat cgtcatgacg cgcgacaaga
    14941 ccttggtgca cggcaagctc ctcatcgacg acaagcccct gatcgagggc gccgtgtccc
    15001 ggccggcctg gcgccacatc gtctacgacg cgccctacaa ccgccacgtg gccgaccgcc
    15061 cccgcctgac ctgggcaaac tggcgcaatg cgctggcggg cgagctctac accgccgaca
    15121 actagctgga ggaggggcag ccccgcaggg gccgagggga ggggcagccc cgcaggggcc
    15181 gagggcccgt cgtcctgggc ccgagcccgg cgacctggcc gccacggccc cacgccgcca
    15241 gcggcccggc agacgcttca agaactcaaa aatcacgcgt tgacgatctt gtagcgccgc
    15301 acgctcatca ataccccgca ggccacgccc atggtcagca aggccgtgcc gccatagctc
    15361 atgaagggca agggcacgcc caccacgggc agcacccccg tcaccatgcc gatgttcacg
    15421 aacacgtaga ggaacatcat catggtcagc gcccccgcca gcaatcgccc gaactgcgtc
    15481 gacccgcccg cggcgatcgt cagcccccgg gcgatcagca ggctgtagag caccaggatc
    15541 gccacgccgc cgtacaggcc gaactcttcc gaatacaccg catagatgaa gtcggtggtg
    15601 cgttcgggaa tgaagtccag gtggctttgc gtgccctgca tatagccctt gccgtacaag
    15661 ccgcccgagc ccaccgcgat catcgactgg atggtgtgga agcccttgcc cagcgggtcg
    15721 gtgctcgggt ccagcagcgt gcacacccgg tgcttctggt aatcgtgcag gccgggccag
    15781 gtcacgctcg ggtcgcacag gcggtcttcg ttgtagacca gcgcgccgac ggcgagcacg
    15841 ccgaccagcg tggccggaat cagcagcttg aacgacaggc cggcgaaata gatcacgaaa
    15901 aagcccgcgc cgaacaccag caccgccgtg cccaggtcgg gctgcagcac gatcaggccg
    15961 caaggcaggg ccagcagcag gcctgccacg aggaagtcgc ggatgcgcac ggccccctca
    16021 tggcgatgga agtaccaggc cagcatcaag ggcacggcga tcttcagcat ttccgagggc
    16081 tggatgcgcg tgaagccgat gttcagccaa cgcgtggcgc ccttgctggt ctcgcccgcg
    16141 aaatgcacgc ccaggagcag cacgatgccc agcacgtaga acggcacggc caggcgcatg
    16201 agctggttgg gcgggatcag ggccacggcc cacatggccg ccagggccac caggaaattg
    16261 cgcgactgct cgacgaaacg cccgtccatg ctgcccacgg cggagtgcat caccgtcatg
    16321 cccagggccg cgaagagcag caggatgaac aggagcggcc agtccagcgc cgtgatcacg
    16381 cgcagcagca cgatgcccaa tcgcttcatt gcgtctctcc tcgcccggtt tgcgcgcgcg
    16441 ccggcgccgc gggcaggggc aggggctcgg gggcaggcgc cgcgggctcg gccggcggca
    16501 cgggttcgcg ccggaacacc gagcccgcca gggccgcgtt gtcgctttcc tgggccaagg
    16561 gccggtggac cgcggccgcg cgggcagcca tcggatcggg caccggcgca tcggccggct
    16621 tgcctgcggc ctgcagcttg tccttgtcga ccagccaata gtcgaacacc ttgcgggcga
    16681 tcggcgccgc ggcgctcgcg ccccagccgc cgttctcgac gatgagcgcc accgcgatgc
    16741 gcggatgctg cacgggcgcg aagccgatgt acagggcatg gtcgcgcagg cgttcgtcca
    16801 ccacgccggc ccggtaccgc gcgccgcgca ggctgtacac ctgggccgtg ccggtcttgc
    16861 cggccgacgg gtaggcggcg ccctggaagg ccaggcgcgc cgtgccgacc cgcgtcacgt
    16921 cggccatggc cgatttcacg atatcgaggt actgctgctt gatcgggatc tggtgatcgg
    16981 gcaccgacgg cgtgggcacg acttccccgg tgcgggaatc gcgtatggca tgcaccaggt
    17041 gcgggcggcg gtacaggccg tcgtcggcca gcgtggaaac cgcctgcccc agctgcagca
    17101 gcgtgaaggc gttgtagccc tgccccaccg tgagcgagac cgactcgccc gcataccagc
    17161 gctgccgttc cttgtccttg taggccttgc gcttccattc ggtggacggc agcacgccgg
    17221 tcctttcgcc ttcgaggtcg atgccggtga tctgcccgaa gccgaactgc ttggtgaaat
    17281 catgcagcgg gttcaccccg atgaccgggc ccagcgagaa aaagtaagtg tcggacgaca
    17341 ccacgatggc cttgtgcatg tcggtcgggc cgtaggccgc gccgcccgca ttgtggaact
    17401 tctgcccgcc gaattcgaag tagcccggat cggggatcag ctcggtgggg ctgcgcttgc
    17461 ccagctccaa cgcggccagc gccacgaagg gcttgtaggt cgagccgatg gggtaggtgc
    17521 cgtacagggg ccggttgatg agcgggtggt cgggcgactc gttgagctgg cgccagttgt
    17581 cggcatcgat gccgtcgacg aacaggttcg ggtcgtacga gggcatcgag acgaaggcca
    17641 gcacttcgcc cgtctccggg tcgatggcca cgagcgcgcc gcgcaggccc tcgaaagcct
    17701 tttcggccac gcgctgcagg cccatgtcca gggacagcat gatgtccgag cccgacacgg
    17761 ggtcggtgcg gcgcagcgtg cgcaccggac gcccgcccgc cgtgatttcc acttcctgca
    17821 cgccggtctt gccgtgcagc acgctttccc aggttttctc gatgcccttc ttgccgatgg
    17881 aatccgtgcc gcggtagttg ccggtttccc cggcgcggtc gaggtcgtcg agatcgctgt
    17941 cggcaatgcg gccgatatag cccaccacgt gcgcggccga cgtgcccagc ggatactgcc
    18001 gcacccagcg cgccttgagc tccacgcccg ggaacatgaa ggcatgcgag gcgaaccagg
    18061 ccgcctcggt gtcgttgagg ttgttgcgca ggaccaccgt ggcatggcgg cccgactcgt
    18121 tgagctggcg cttgaacttg cggcgatcgc tcgggctcag gtagaccact tgcgacaggc
    18181 ggtcgaacag ttcgtcgaag tcgtcgatct cggagggcgt gacctcgagc gtataggtgc
    18241 ggaaattgcg cgccagcacc tcgccgttgc ggtcgagcac ttcgccgcgg cgcggcggaa
    18301 tcggcaccac cgcgatgcgg ttgcgatcgg cgcgctcgga aaagccctgg tagcgttcga
    18361 cctgcagcac ccagaagcgc gacaccagca cgccgaagca cgccagcacc acggccgcgc
    18421 ccgccagggc gcgcaggcga aagcgctgct tctgttgaag actggtgttt ttgaactcga
    18481 acatcgtggt cccgccctag accgcggaag attccacgtc gtccaggcga cgctgcggca
    18541 attgcagcac ccaacccacg ataggccaga gagcggcggt gatcaaggcg ctcagggtcc
    18601 agccccagcc cgaccaatgg ccggccagcc aggcgacgac cagatgcgtg cacagcggcg
    18661 cgatcaaaaa gaccggcagc atgtgcacgg cctggctcca caggtcgaag cgctgcaggc
    18721 ggcggtccag cagctgcgcg ccataggcca ccaggacgta ggtcagggca tggccgccca
    18781 gcagggccgt gtcgtgcacg tcgagcagga tgccgaagac gaaagccgtg accaggccca
    18841 cgcggcggcg ttcgtgcgcg ctccagaagg ccagcaccag cgccatgatg tcgggcgcgg
    18901 ccggccagtc gcgccagggc agcatcgacg ccagccacac cacgatcagg gtgccccaga
    18961 tgaacaggcc gcgcgccggg cgcaccagcc ggtcgggctg cgcattgccg agcaggccca
    19021 gcaggcggcc gggaacgggc gcctgcggat ccggggacga cggattattc ttcatcgtca
    19081 tcgtcggact cctgcatttt catggcctgg tccacgtcga cctgcagcac caggaagtgg
    19141 cggtagcgct cagggtgcga cagcggctcg gccacggcct gcgcgaagcc cgaggccgag
    19201 ttgcgatcga cggacgtgac cttggccacc tgcagcccgg ccgggaacag gccgcccacg
    19261 ccgctggtga tgagcacgtc gccttcgcgc acgtcggcat tggccgacag gtagcgcact
    19321 tcgacctcgc ccggcgtgtt cgtgccgaac gcgatgagcc gcagcccgtt gcgcagcacc
    19381 tgtaccggga tcgacacgcg gtcgtcggtg agcatcgcaa gctcggaggt catgggcgtg
    19441 acgcgcacca gctggcccac cacgccgccc tcgtcgatca cgggcatgcc cgcggccagg
    19501 ccggcctggc tgcctttgtt gaacaccagg cgctgctggc cgctgccgct gggcggctcg
    19561 taaagcacct cgaccaccac ggccggctcg ggcgtgtggt cggtgatgcc gagcagccgg
    19621 cgcagctggg cgttttccgc ggccacctgc tgggcatggg tggacacctg cgccagttcg
    19681 atgcgctggc gctgcagggc ttcgttttcg ctgcggatga ggttggcggc gttgacccac
    19741 tcgtcgacct gctcgaccag gtcgcggggc gccatgacga cgcgctggaa aggatagagc
    19801 gcgatggcca ccgcgcggcg caccggctcg agcacgcgca tgcgcgcatc ggccagcatc
    19861 agggccaggg ccagcacgac cagcaccgcc agtcggactt cagcgggcgg gccgcgccga
    19921 aacagcggag gggtcgtgct ttgtcgttgc atggtgtcag cctggtcgat gaagaccgcg
    19981 gcgggtatgc cggccgcatg cgggatgacg cgtggcggcc ggcggcacgg acttagtcgt
    20041 tgatgaagat ggcgcccagt ttttcaagat gctcaagcgc ttcgccgcag ccgcgcacca
    20101 cgcaggtgag gggatcttcg gccaccagca ccggcaggcc ggtttcttcc tgcagcaggc
    20161 ggtccaggtc gcgcagcagc gcgccgccgc cggtcagcgc aatgcccttg tcggtgatgt
    20221 cggcgcccag ttcgggcggg gtttgttcca gcgcgatctt gacggccgag acgatctggt
    20281 tgagcggatc ggtcagcgat tcgaggatct cgttggacga gacggtgaaa ctgcgcggca
    20341 cgccttcggc caggttgcgg cccttgacct cgatctcgcg gacttcggag ccggggaagg
    20401 ccgagccgat ttccttcttg atgagttcgg cggtgggctc gccgatcagc atgccgtagt
    20461 tgcggcggat gtagttgacg atggcttcgt cgaacttgtc gccgccgacg cgcaccgaac
    20521 ccttgtagac catgccgccc agcgagatca cggccacctc ggtggtgccg ccgccgatgt
    20581 cgaccaccat cgagccgctg gcgtccgata ccgccaggcc ggcgccgatg gccgcggcca
    20641 tcggctcctc gatcaggtag acgtgcgagg cgcccgcgcc cagcgcggat tcgcggatgg
    20701 cgcggcgctc gacctgggtg gagccgcagg gcacgcagac gatgatgcgc ggactgggcg
    20761 ccagcatgtt gcgggggtgc accatgcgga tgaactgctt gagcatctgc tcggtgacgg
    20821 tgaagtcggc gatcacgccg tccttcatcg gccggatggc ttcgatgttg ccgggcacgc
    20881 ggcccagcat ctgcttggcc tcgcggccga cggcctggat gatcttcttg ccgttggggc
    20941 cgccttcatg gcgaatggcc acgacggacg gctcgtcgag cacgataccc ttgccgcgga
    21001 cataaataag cgtattggcg gtaccgaggt cgatcgccat gtcgctggaa aaataactac
    21061 ggaggaatcc gaacatgtga actcagcgag attcagggaa ttagggtcat gacgctgggt
    21121 tttacccgcg gtgctacccg ccggcttacc tacttttatc ggtcggacat gtttggacat
    21181 gccgccttcc ggtcgcctgg ttgccggacg tacaatgaca tcagcgatta aaccgtgaat
    21241 gataacttat aattttgcgg gaaatagcgc gaaaatcccg gctattcatg gtttgtaacg
    21301 gcttagactt gccgcatcct gcggggctcc gtcccgcttt tattttcctt tcattcccat
    21361 ggccctgaac gaacaagacg tggctcgcat cgcccggctg gcccgcatcg agctcaccgc
    21421 cgagcagcgc ggccgcgccc aggcggagct caacggcatt ctgcacctga tcgagcggct
    21481 gcaggccgtc gacacccagg gggtggagcc gctggcccat ccgctgtcgg cccacgaaga
    21541 cgtggtgctg cgcctgcgcg ccgacgccgt caccgaaccc gcgtccgagg cgcgccgcag
    21601 cgaactgctg gccaatgcgc cggacacccg cgacggcctg ttcctggtgc ccaaggtcct
    21661 cgaataattt cgcacccaca ccgtgcggcg cgccgtgcgc ccgcccccta tcgaatgcat
    21721 accacccatg gcgacttccg ctctgcataa cgaattcggc gacatcgccg gcctgcgcga
    21781 ggcgctggcg cagcgccgcg tcggcgcggt cgagctggcg caaagcgcgc tggccgccgc
    21841 cgaggcggcc gcgcacctca acgccttcct gcacatcgac gccgacctca cgctggccca
    21901 ggcgcgcgcc gccgaggcgg ccatcgccgc cggcaccgcc ggcccgctgg ccggcatccc
    21961 catcgcgcac aaggacgtgt tcgtcacgcg cggctggcgc accaccgccg gcagcaagat
    22021 gctcgaaagc tatgtgagcc ccttcgacgc caccgtggtc gagcgcctgc ggcaagccgg
    22081 cgccgtgtcg ctgggcaagc tcaattgcga cgagttcgcc atgggctcga gcaacgagaa
    22141 ctcggcctac ggcccggtgc acaacccctg ggacgcgaac gccgtgcccg gcggctcgtc
    22201 gggcggttcg gcggccgcga tggccgccgg cctggccatg gccaccaccg gcaccgacac
    22261 gggcggctcg gtgcgccagc ccgcctcgct gtgcggcgtc agcggcatca agcccaccta
    22321 cggcacggtg tcgcgcttcg gcatgatcgc ctacggctcc agcctggacc aggccggcgt
    22381 gttcgcccac ggcagccgcg acctgctcga actgctcgac gtgatgagcg gcttcgaccc
    22441 gcaggacgcc accagcctgc aagcctgcga cggcgcggcc aacgtcccgg gccgcgtgcg
    22501 ggcggcattc gacgccgcgc aagccgggtt cgacggcgcc ggcagcctgc ccttgcgggg
    22561 cctgcgcatc ggcgtgccca aggaattctt cggcgcgggc ctggccgccg acgtcgccgc
    22621 gcccgtcgag gcggcgctca agcagttcga agccctgggc gcgcagcgcg tggagatctc
    22681 cctgccgcgc accgagctgt cgattccgtc ctactacgtg atcgcgccgg ccgaagcgtc
    22741 gagcaacctg tcgcgctacg acggggtgcg ctttggccat cgcgccgccg actacgccga
    22801 cctgaccgaa atgatcagcc gttcgcgcgc cgaaggcttc ggcgaagaag tgaagcgccg
    22861 catcctgacg ggcgcatacg tgctgtcgca cggctattac gacgcctact acctgcaggc
    22921 ccagcgcctg cgccgcatga tcgcgcagga tttccagctg gccttcgcgg accggtgcga
    22981 cgtgatcatg gggccggtat cgcccaccgt ggcctgggac atcggcaaga agcgcagcga
    23041 ccccacgtcc gaatggctca tggacatcta cacgctgagc ctgaacctgg tgggcctgcc
    23101 gggcatgtcg gtgcccgtgg gcttcggcgg cgagcggcgc aatcgccccg tgggcctgca
    23161 ggtcatcggc aactacttcg acgaaggccg gctgctggcc atcgccgacc gctaccagca
    23221 agtcaccgac tggcataaac gcaagcctgc aacgcaggcc tgacaaaaaa ccgttcacga
    23281 acattcgggc tgccgccgcg cagcggcgca gcccgacgat aggtaccgac atggattggg
    23341 aaatcgtcat cggcctggaa acgcacacgc agctttccac cgactccaag atcttctcgg
    23401 gcagcagcac ccgcttcggc gccgcgccca acacgcaggc caatgtggtc gacctggccc
    23461 tgcccggcag cctgcccgtg atgaaccgcg gcgcggccga acgcgccatc cggttcggcc
    23521 tggccgtggg cggcgccgtg gcgccgcgct cggtcttcgc gcgcaagaac tacttctacc
    23581 ccgacctgcc caagggctac cagatcagcc agtacgagct gccggtggtg caaggcggct
    23641 cggtctcctt cttcgtcggc gaagaagaaa aaaccgtcaa cctcacgcgc gcccacctcg
    23701 aagaagacgc cggcaagtcg ctgcacgacg acttcgcgct gggcaacggc aagccggcca
    23761 gcggcatcga cctcaaccgc gccggcacgc cgctgctcga gatcgtcacc gaacccgaga
    23821 tgcgctcggc cgccgaggcc gtggcctatg cgcgcgcgct gcacagcctg gtgatgtggc
    23881 tgggcatctg cgacggcaat atgcaggaag ggtcgttccg ctgcgacgcc aacgtgtcgg
    23941 tgcgccccag gggccagaag gaattcggca cccgcgccga gatcaagaac gtcaactcct
    24001 tccgcttcct ggagcgcgcc atcctgttcg aggccaagcg ccagatcgaa ctgatcgagg
    24061 acggcggcac ggtggtgcag gaaacgcggc tctacgattc ggaccgcgac gagacgcgca
    24121 gcatgcgcag caaggaagac gcgcacgatt accgctactt ccccgacccc gacctgccgc
    24181 cgctggtgat cgcgccggaa tgggtcgaac aggtgcgcgc cgccatgccc gagctgcccg
    24241 acacgctgcg ccagcgcttt gcggccgact acggcctgtc ggcctacgat gcggcgcagc
    24301 tggtgtccag ccgcgccatg gccggctact tcgaggaaac ggccaagtcg ctgccggccg
    24361 gccaggccaa gctggcggcc aactgggtca tgggcgaagt cgccgcgcag ctcaaccgcg
    24421 aagaaaagga catggccggc tgccccgtgc cggccgcggc gctggccgcg ctgatcggcc
    24481 gcatcctgga cggcacgatc tccaacaaga tcgcccgcga agtgttcgcc gccatgtggg
    24541 ccggcgaaca cggcggcgcg cccgatgcca tcatcgacgc gcgcggcttg aagcagatca
    24601 gcgacacggg cgccattgcc gccatgatcg acgaagtgct ggccgccaac ccggccatcg
    24661 tcgaggaata ccgcgccggc aagcagaagg ccttcaactc gctggtgggc cagatcatga
    24721 aggccgcccg cggcaaggcc aacccgcaac aagtcaacga actgctcaag caaaagctgg
    24781 atagctgagg ccgtgagcaa agcctatgcg cgcaaagccc tggtcgcctc caccgtcggc
    24841 tacgcgctgg atggtttcga cctgctgatc ctgggcttca tcctggcggc catctcgaaa
    24901 gacctcgggc tttccagcac ccaggcgggc ctgctcgtga cctggacgct ggcgggcgcg
    24961 gtggtaggcg gcatcctgtt cggctggctg gccgacaggc tgggccgagt gcgcgtgctc
    25021 aactggacca tcctgatctt tgccgtgttc accggattgt gcgcgctggc ccggggctat
    25081 gacgacctgc tggtctatcg aaccatcgcc ggcctgggcc tgggcggcga gttcggcatc
    25141 ggcatggccc tggccgccga ggcctgccgc ccggaacaac gggcccgcat gacctcgttc
    25201 gtgggcctgg gttggcaagg cggcgtgctg gccgccgcca tactcacgcc actgctcctg
    25261 ccgcacatag gctggcgcgg catgttcgcc gtgggcgtgc tgcctgccgt ggcggccttc
    25321 atcatccgcc attacattga agaacccagg ctcttcctgg atgcccgcca ggcgccggcg
    25381 gtcaattcgt tcaaactgct ggtggccgac agcgccacgg cccgcaccag cctgggcgtg
    25441 gtcatcctca cgtccgtgca gaatttcggc tactacggcg tgatgatctg gatgccgtcc
    25501 tacctgagca accagttcgg ctatacgctg accaagtcag ccggctggac cgccgtcacg
    25561 gtaatcggca tggcgcttgg cctatggctg ttcggccaac tggccgaccg cgtgggccgc
    25621 cgtcccatct tcctgctgta ccaggcgggc gccatgctga gcgtgctggc ctattcgcag
    25681 ctgcaatcgc aatatgcgct gctcgccggc ggcgccgtca tgggactgtt cgtcaacggc
    25741 atgatcggcg gctacggcgc gctgatcgcc gaactctatc ccacgcgggc ccgcgccacg
    25801 gcgcagaacg tgctgttcaa catcggccgg gccgtgggcg gcttcgggcc gctggcggtg
    25861 ggctcgctgg ccgccagata ttcgttccag gtcgccattg ggctgctggc cgtgatctat
    25921 atcgccgaca tcctggccac gctgttcctg attcccgagc gcaagggctc ggcgctggaa
    25981 tagcggataa tgtcggggtt cgatttttcg ctccggatat gcccatgacc tctgccacgc
    26041 ccaccgattt cgttcgtttc gccctggacg aaggcgtgct tcgcttcggc agcttcaagg
    26101 tcaagtccgg ccgcatcagc ccctacttct tcaacgccgg cctgttcaac ggcggccgct
    26161 cggtgggccg cctggccggc ttctacgccc aggcgctgct ggattcgggc gtggccttcg
    26221 acatgctgtt cggcccggcc tacaagggca tccccctggc cacggccacc gccgtggcgc
    26281 tggccggcca ccccggcatg cagggccgcg acgtgccctt cgccttcaac cgcaaggaag
    26341 ccaaggacca cggcgagggc ggcaccctgg tgggcgcgcc gctcaagggc cgcgtcgtca
    26401 tcatcgacga cgtgatcacc gccggcacct cggtgcgcga atcggtggag atcatccgcg
    26461 ccgccggcgc cgaacccgcc gccgtgctga tcgcgctgga ccgcatggaa cgcgccggcg
    26521 ccgacgacgc cctgtcgtcc cactcggccg tgcaggaagt cgccaagacc tacggcatgc
    26581 cggtgatcag catcgccagc ctgaaggaca tcatggcgct gctggagcag gatccggcat
    26641 tcgccgaaca ccgcggcgcc gtcgccgcct atcgcgcccg atacggagtc ctctgatcat
    26701 gtttcgagtc atctcggcca acctcaacgg catccgctcc gcctccacca agggcttctt
    26761 cgactggatg cgcaagcaag acgccgacgt catcggcgtg caggaaatca aggcccagca
    26821 tgaccatgtc accggcgtct tcgaccgcca cgacaagctg gccggctatt tccactatgc
    26881 cgagaagaaa ggctattcgg gcgtgggcct ctacacgcgg catgagccca gcgcggtcat
    26941 ccgcggcttc gacgccacgg aattcgacgc cgaaggccgc tgggtcgagc tgcgcttcga
    27001 cacgcccgag cgcaagttca gcgtgatcag ctgctacttc cccagcggct cgagcggcga
    27061 cgagcgccag caggccaagt accgctttct cgaccggatg tacccgcacc tgatgcgcct
    27121 gaaagccgag cgccagttca tcctgatggg cgacatcaac atcgcccaca aggaagccga
    27181 cctgaagaac tggaaaggca acctgaagaa cagcggcttc ctgcccgaag agcgcgcctg
    27241 gatgacgaag ctgctgggcg aaggcggctt ggtcgacgtc taccgcgccc tccaccccga
    27301 caccaccgac gcctgctata catggtggag caaccgcggc caggcctatg ccaagaacgt
    27361 ggggtggcgc atcgactacc agctggccac ggaccgcgtc gcggcctgcg cgcgcgcaag
    27421 caccatctac aaggacgcaa agttcagcga ccacgcgccg ctgacggtcg actacgactt
    27481 ctcgctctaa ggcctgcccg ccggtcctgg tcccgggctt gccggccctt attcgaccgg
    27541 cacgtgcgag tcctgcggcg gcatgtagaa gcagtaccgg ttcttgcccg ccgccttggc
    27601 ttcatagcgg gccttgtcgg cccggtccag cagttgcccg gcctccagcc cgtcatccgg
    27661 gtataggctg atgccgatgc tggccgtcac ctgcaggtgg tggccgttga ggcggatttc
    27721 ctggtcgaga tgggtcagca gcttgcgggc gacgcccgcc acgtcgtcgg cctcgtggat
    27781 gtcttcgagc aggatggcga attcatcgcc ccccagccgc gcaatgccgt cgctggcccg
    27841 caggcggcgt tccagccgcc gcgcgatcac ccgcagcacc tcgtcgccca ggctatggcc
    27901 cagtgcggta ttgacctgcc tgaaaccgtc cagatcgaga aacagcaccg ccacccgcat
    27961 cgcgttgcgc tgggcccgga ccacggcatg cgccagccgc tcgttgaaat gcagccggtt
    28021 gggcaggtcg gtcagggcgt cgcgcatgag cagttcggcc atgcggccct cgacccgggg
    28081 ccgctcgctg gcatccagtc ccatgaccac gtagttggaa tagccgccca ggtgatcgcc
    28141 gatccccgcg atcaagaccg actcgcggta gatcgcgccg tttttcgtgc ggttccagag
    28201 atcgcccgac cagcgcccct tctcgcgcaa tacctgttcg agctcgcggt attgcatctc
    28261 ggactgccgg cccgagttga gcatgctcag cggcttgccg gccgtctcgc tcatcgcgta
    28321 gccggtcagg cgggtgaagg ccgggttcac gcgcacgatg cggccgtcat ggtcgagcaa
    28381 ggccacgctg ctttgcgtgt tttcgaaaat gcggttggcc atccacaggt gctggtgcgc
    28441 ctcgctcagg gcctcgttgc gatcgcgcac caggcgatag accaccagcc ccgagacgaa
    28501 catcaacaag cccaccagca gcaggccgac ccagatgatc gcgcgaaata tcgcgctgcc
    28561 ctggctggac agactctccg cctctcgaag attgctggcg atgcattgcc ccaccaggcg
    28621 gtcgaatccg ttgaggaatt gctcgttgcc gttgcgccag ttcgcgatcg cggcggcgtc
    28681 cctgttgccc agcaggctga gcccttcttc catcatcgcc ttgaagcgcg gcagggcgtc
    28741 gcgcagattg tcggcgatga cctgctcgtc gccgcccgat acgccgctcg ggtagtagag
    28801 gctccacacc gtgctcatgc gcaccaggct caggtggatc tcccgctgcg actggatgac
    28861 ggaatcggta tcgctcagca gggccaggcc gcgccagagc tggcggcggg cgtccagttc
    28921 ggccgcgcgg aaggcgtcga gctggctgat gggcaccatc gtgccgctat agagcgccct
    28981 ggcgtatccg tccagccgca ggctgctcag gatgctgccc gacagcagca gcaacagcaa
    29041 caccgctccc acggccagcc aggaaaccag gtagatccgg cgtatcgaac gtaaaaaatg
    29101 cagcatggac agcccaaaga ccggccccag ggtcgggccc aaggcccata tattcgcaaa
    29161 tttgatacta atctgcgaaa agcccgccgc attcaatacg caggaatcgt gaagtcccgg
    29221 cgaaaccggc gctggatgcc attccgtccc ctattgcaat gccggggagg ccagtcactc
    29281 ctggccctgc ttttcgagct ggtcgatctg cgcgcgatgg acgcgcagca acagcaggcc
    29341 gggcacggca atggccaccg tcccgaggaa aaagcccggc cagcccaggt gctcgaccag
    29401 ggtgggcgtg agcggcccgg ccaggtaggt gcggcccacg gccgacagcg ccgacagcag
    29461 ggcaaactgc gtggccgaga actcctggcg gcacagggcc atcaagaggc cgacgaaggc
    29521 cgccgtgccc aggccgccgc acaggttctc gatgccgacg ccgccggcca tcagccagac
    29581 gtgcttgggg ctgaccgaga tcagccaata gcccaggttg gacaccgcct gcagcagccc
    29641 gaaggccatc agcgaccgat acaggcccca gcgcgacatg atcgaaccgc cggccagcgc
    29701 ccccacgatg gtggccacca ggcccagcac cttgttgacc gtgccgactt ccgtggcggt
    29761 gtagcccgcc gcccggatca ggaaagtggt ggacagcgcg ccggcaaagg catcgcccag
    29821 cttgtagagc acgatgagca gcagcagccc gaccgcgccg ggccggctga agaattcacg
    29881 gaagggctcc accacggctt gccccaggtt gcgcggcgcg gcggccggcg actcaggttc
    29941 gggcgcccat agcgtggcgg ccatgcaggc cagcatcagc ccccccatga gcagataggt
    30001 gtggccccaa cccagccagc tgtcggccag caccagcgcc aggccgcccg agacgatcat
    30061 ggccaggcgg tagcccagca ccttgaccgc ggcgcccgcg ccgcgctccg cgctgtgcag
    30121 gacgtcggtg ctgtaggcgt cgaaggcgat gtcctgcgtg gccgacaata ccgccaccag
    30181 cacggccagc gcggccaggt ccggcaaggc cgagccgggc gagagcaggc tcatgcccat
    30241 gatggccagg gccagcagca attgcgtcag cagcatccag ccgcggcgcc ggcccagcca
    30301 gggcggcgca tagcggtcga tcagcggggc ccacaggaac ttcagcgtat aggccgtgcc
    30361 caccagcgtg agaaagccga tgctctgcag cgagacgttc tcgaccgtgg cccaggcctg
    30421 cagcgtgccg ctggtcagcg ccagcggcag cccgctggcg aagcccaggg ccaggagcgg
    30481 cgccacgcgg cggctgaggt agacattgcc tgccggcacc gtggggctcg cgtccgtcat
    30541 gggcgtgttc ctttgcaaga gggcggggtc agtccgcgga gaaacgatac atggccaggg
    30601 tgctgcgcca ctgcggcagc acgcgcacct cgcgcgcctc gttgaaggtg gcgcgctcga
    30661 ggatgcgcag gcccacctgc gcggccaggt cttcgaagtc gcgcaaggtg cacaggtgga
    30721 tgttgggcgt gttgtaccac tggtagggca tctgccccgt caccggcatg cggccgcgca
    30781 ggatggacca gccgtgcggc cagtagccga aattgggaaa ggacacgatg ccgtagcgcg
    30841 ccacccgcgt catttcgcgc aggatgtgcg tggtgtgatg catggactgc agggtctgcg
    30901 acagcaccac ggtctcgaac tgcttgtcgt cgaacagggc cagcccgcct tcgaggttct
    30961 gctggatgac ttccacgccg cgctgcacgc aggtgatcac atggctgtcg tcgatctcga
    31021 cgcccgcgcc gctgacctgg cgattgtcgc gcagccaggc cagcaggtcg ccgtcgccgc
    31081 agcccaggtc cagcacccgg ctgcccggct cgatccagtc cgcgatgcgg gccaggtcgg
    31141 gacgcaggac gaccggtccg gaacgtaccg acaccgggct cattccgcca cccccgacac
    31201 ccccgccgct tgcggcaaac ccagttcgcg cgcaatgcgc tcgcaatagc cgcgcaccac
    31261 cgcgtggtag cgcgtatcgt ccagcaggaa ggcgtcgtgg ccgtgcggcg cgtcgatctc
    31321 ggcataggtg accgggctgc cgttcttgag caaggcgcgc acgatctcgc gcgagcgctc
    31381 gggcggaaag cgccagtcgg tcgaaaagga caccagcagg aagtccgccc tggccggcga
    31441 caacgcgcgc gccaggtcgc cgcccgcatt gcgcgccggg tcgaaataat ccagcgcgcg
    31501 cgtgatcagc aaataggtgt tggcgtcgaa atagcgcgag aacttctcgc cctggtagcg
    31561 caggtaggac tcgacctcga actccacgtc gtagccgtag cgataggcgc cgtcttccgc
    31621 gggctcgcgc tgcgcgcggc cgaatttctc ggccatgtcg tcgtccgaca ggtaggtgat
    31681 gtggccgatc atgcgcgcca ccgacaggcc gcggcccggc acggtgtcgt gcgcgtagta
    31741 atcgccgccg ttgaagtcgg ggtcggtgat gatggcgcgc cgcgccactt cgttgaagcc
    31801 gatgttctgc gccgacaacc gcggcgtgct ggcgatgacg atgcaatgcg ccacccgctc
    31861 gggacaggtg atggcccagg acagcgcctg catgccgccc agcgagccgc ccatgaccgc
    31921 ggcgaagcgc tcgatgccga agtggtcggc cacgcgcgcc tgggcctgca cccagtcctc
    31981 gacggtcagc acggggaaag ccgcgcccca gggcttgccg gtggccggat tgaggctggc
    32041 cggccccgtc gaaccgaagc acgagcccag gttgttgatg ccgatgacga aaaagcggtc
    32101 ggtgtcgacc ggcttgcccg gccccaccat attgtcccac cagccctggg actggtcgtc
    32161 ggccgcgacg ccggccacat ggtgcgaggc gttgagcgca tggcagatca gcaccgcgtt
    32221 gctgtgttcg gcgttgagcg tgccgtaggt ctcgacggcc agttcatagg cgtcgagctg
    32281 ctggccgctc gccaggggca gtggctcggt aaaggcgagg aaaatcgggg aaaccacgcc
    32341 gacggaaccg ggagccggcg cgggccggct tggcgacttc ggggcggggg ccggcgcggc
    32401 atgatcggac gcgcctgcct cgtgaggggc aatgagggtc atacggacct ctttagcagg
    32461 atttatagag gcgcccgcaa gcggatcagg caaatcggcg ccgttcaatg ccgagattct
    32521 agcatcagcg cccccgggcg ctatgccgcg gacgatcacc cgccccatgg ccggcggcat
    32581 ggacgctgtc cgcgggcggc tggcgcggag aaaatgccat cgtcgccctt tggcaaggaa
    32641 ccgcccctca ccatgcccga aatcctgcta tccgaccgcg aacgggactg cctgcactgg
    32701 gccgccctgg gcaagaccag ttgggaaacc ggccatattc tcggtttgag ccagcacacc
    32761 atcaacttcc atgtacgcaa cgcgtgcgtc aaactgggcg ccagcaaccg ccgggcggcc
    32821 gtggccatgg ccctgcggct ggggctgcta tgatgggtaa tctatgccac tcagtggcat
    32881 aatatgaaaa caaggcgagt atttaaaact cgcttttttc aacgctggat gcgcaagcca
    32941 gggttgactg acgccacgct gcgtgaggcc gtacaagaaa tgtcagcagg actggttcgc
    33001 gcagtagcgg atgggacgcc acaggagatt tgtgatggca accaaggcta aaagccggat
    33061 tctcgatacc gttcatgaga cggctgtgga tctgcatcgg ctcggattca tcgacaagcg
    33121 caagatgcgg aagtacgaag cgctatgcct tgagccggtg cctgaatatg atgcggcaaa
    33181 gattcgggca ctacgcgccc gattccatct gagccaggcg gtcctggctg ccgcgctcaa
    33241 cacgagttca tcgaccgtcc gcaaatggga agccggcgat aagcggccaa gcggcccttc
    33301 tcagaagctc ttgaatctga tcgatcggaa agggcttggg gccgttcttt aacccaattg
    33361 gctcgtgatt tccgccgctc tggaacggcc tatggaaaac gctgcgcaat ttcccatggc
    33421 ttttcgtatt agtaggcgtc tgctttcgac cctgcgcggc cgctcgtagc gctttcgctg
    33481 acatctgctg tgcagtgatc tcggtctgta ccttcgtggc gcgatgcacg agacgcatct
    33541 gcctggtatg tggctcgggc cagcgtagcc cacgttatcg cagtggcgtt cttgccaaaa
    33601 gcaaaagctg cctagttcaa gcaacacttc ttgtatttaa gcccgcttcc gcaatggcac
    33661 ggatcgttgg gacctaactt caaccgcctg acctctcgcg aatgcctagg gaatgtatct
    33721 cgcactttgg ccggcggcgt catgtcagcg gtgagccgat ccatctcgtc tgatgcctcc
    33781 cacgggtagt ccaacgtgac gccgaactgg actttcccgg cggggtccac cgagacgccg
    33841 aaccattgcg cggcacgctg ctgatacttg cgcatcgtgc agtacgtctc cagtacggca
    33901 atcgcctccg gagatggagt gggattgcaa tggaacgtga ccccgccagg aggtgcactt
    33961 gatgcgagcg tgatgtcatg tcgcttaccg tcgagatgcg cttgtcgcgt gatggtctca
    34021 accacacgat ggacattcct gcacgcatcc tcgtccatag ccagcaagtg aaaaccgagc
    34081 tcaagagttg cagggtcagc cctcgcctca atctctttaa taaggctctc atagcgagtc
    34141 ccacgcatct tcgtgaggat gcccaccggg gtgtcatcgc caggcaggtt gtcccggcgc
    34201 accagcattg ccgtgtccag gccctgcgcg acggagtcat cgagcataaa gcagtcgtat
    34261 ttctcatcca gccacaggtt ctgtttaagg tggtagccga gaacggtcaa ttcatgccca
    34321 gtcatcagct tgtccgcgac ggctacccgc agcctcacgt agcccaacag ccgcaaaggc
    34381 gtcgccagca tctccgtcaa cgtatcgaga aggaacacgt ccatcacgaa cggcgggcga
    34441 atgacatcgg tggtctgaaa cttcaacgac tggctggcct gaaaggcaag cgccggatag
    34501 tgctctgaca ctaggccgaa caggaagacc tccttgatgg tcggaggaag ctcgacgtgt
    34561 ttcccattgt catcaacgag tcggcaccct ccagcaagca tctcgttcgc gcacgcccac
    34621 ccttggtcat acgagtcttg gatggcagcg gagaagtcag ccttcaactg gctgtcgttc
    34681 cccttccgag cggcaatggt cagcttcttg gccttggcct gcacaatgat gaggcggtcc
    34741 ccatagatca ccagaacatc cgcctcaccc acgatgtcct tgccacgatg caggttcacg
    34801 ttcgtgtgaa catgctcgcg gccgaagact ccgaccaacc ggcgcgcagc gaactgctca
    34861 gtaaaagctc cacgatgctt ggccgctatc gggcggtagg gcttgtcgtc ccacatccaa
    34921 aagaacgggg actcgtacat agcctcgtag atggcgtagt gcgagaagag aagcacagtc
    34981 ccacggtcag taggcagcag cggtcgagcg gcgacctcgt tgaagtcgcc cacctccttg
    35041 aaccctgcgt tgtctcccgc aaagagcaag gcgcggaaaa aggcttcaac cacgtccttt
    35101 gacgcaccgc tgcgaaacgc aacctcatct ggtgcgaact caaaggcagg cagccaactc
    35161 gctggagagg ctttggctgc tctagcacct acaaggagtc gcgtaccctt ctcgtccatc
    35221 aagacacaca tcgtctttgc gatgctccgc gcttggctgg atgtgaagcc tttggccttg
    35281 agcatccatt cgtcatcggc gccgtacttc tcgggcagaa gatctctgta ctggaacgca
    35341 taggctgact cggtgccgta gaagatgggt tctcgcattc ccggcccacg ccacgggtca
    35401 ggcggttcag tgcctgcttt catcgcctcg aacatggctg cgaacatcgg gaaggacatc
    35461 gcttcgtgca gctcatgcat gagggtgtct gtacgcttga cgtagccctc gagcacatca
    35521 ggctgctgca gagccaggtc caacggcttc ttcgccatca ggccaatgat tgtcgtcagt
    35581 tccgcccgat tcagccgttc tctgctaaat agccgatcca tgtcggccgg cttcagcttg
    35641 ccctggatgt gaataacgtt gtcgcgatga cagatgtgcg cgaccgcatg agcgtatccc
    35701 ggggatgcgg caagtgctgc cagggcctca agaatctgcg actcttcccg cggcttttcc
    35761 ataactaccc ctttgaggct ctcaagccct gttccccggc gcatcttcgc gacgattttc
    35821 cgccctgctt aggctgctgc ctaggagcgc tcaagggcga actctcggat gtcatgttct
    35881 acaggtggcg gtcgaagttt tttcggtctt cagcgacaga ttttaacgat agctcccgct
    35941 gatccggtga gttgcgaacg actcagcctg aagcagtcgg ccaagcgggt gttgctacga
    36001 gcagttcctg gccaaaaaac cgacatggct agtgataacc acacattgtc gctagctagc
    36061 caatcgccgc caccctaagc ggacaaccgt taaaaagccc gcttgggcga tccggctaat
    36121 caatccgata acgggctgac cgttgatcga tccacctgcg gttcacattc ttaacccgag
    36181 ggcgaaagta ttctaggcta ctgctttaca acaaacctgc catcaacgcg tcgcgccaga
    36241 tcgctaacag cattactacc cgccgccgaa gcatcgctta tcctacgaat tcatgggcat
    36301 attgcatatg gacacttcca tgctggataa cgccctcaat ctttcactcc tgtcctcata
    36361 cgaccgtcac cgcaacccgc ttattcacat aggaactccc cggttgacgc gacgctccca
    36421 cacaccccag aattcgagct acgccccacc taggggtcaa atagtagtca aacggcagga
    36481 caactatgca agtacgtgaa atatcaatag ctgacgcact atccctctcc aagcaaccag
    36541 aggggcattt ctatgaccgg aaggctgcgc aggttaaagg cgtcaagctt caaaaaatcg
    36601 tctgcgcctt cgctaatgca gatggcggcg atgtctatgt aggaatcgct gacgataaag
    36661 atgaggcaga cccaacgaag cggtgggttg gttcgccgac gatggaagag tttaatcaac
    36721 tcattcaatc attgatggag atgaggccat cacctcctat gacaatggag tttctcaagt
    36781 caaccctttc aagcaactac gtacttcata tccaagtcga taaaagccag tctgttcatc
    36841 aaacaactga cggaactgtg tacgaacgga agggggcaca gtcactccca attaaagaag
    36901 ccgagcgaat cacagcgttg ggatttgcaa agggcgcatc ctcctacgaa gacatgcaag
    36961 ttgactcagc agacgctgaa gatgtggtgg acgccgacga gattaaggtc tttttgaacg
    37021 aatactcgcc aaagacagac cccctagatc tgtctataaa taagggcctg attgaccgta
    37081 gaaccttcaa acccaaagct tgcggcctcc tgcttttcgc aaacgaccca agtggcgtag
    37141 tacctaagaa gtgctccgta aagatcgtcc gatacgaaac gaaagaagat gatcctgaac
    37201 gagatcattt agcttttacg gaaaccgttg aggggccact tttccctctg atcaaaaaat
    37261 cagttgcgcg cgtgacggaa attatgtcgt ccatcagtgt ttggacaacc gagggcgcca
    37321 aatccatgga atacccccca gagacgcttt gggaaattgt ggtcaacgca gtcattcatc
    37381 gtgactactc agtgtcggat gacgtacaaa tattgatctt cgacaatcgc attgaaattc
    37441 tcagccctgg ccgtcttcct ggcttcgtaa gtcgcgacaa tattctggac gttcgctacg
    37501 cgagaaaccc aaagattgtc ggaatgcttt ccaagtacaa agacgcgcca aataaggata
    37561 ttggcgaggg cttaaatact gccttccaga aaatgaaaga atggaagatg cgaaatcctg
    37621 aaatcattga agaagacaac tacattcgcg tcgttattcc ccatgcatcg cttgccacgc
    37681 ctcaagaagc aatcatggaa tttctttcca ataaccaaac catcacaaac aagcaggcac
    37741 gagacattac tggcatcaag agcgagaacg ctgtcaaatc tgagttctac aagctgcggg
    37801 acacaggcag gattgagatg attcctgagc tgaaaggcaa caaggcagcc tggcgtttag
    37861 tggcagctac tagttagagc ttgccgtcta acaattaatt caagccgaac ccgcttcgcg
    37921 gctcggctga attctggcgt tctgaacgtc cactactggc cgagaacggt ctcccgcttc
    37981 tggctgcttg cggcccactc cgacctaccc ggccttttgc ttgtcgccag catcttgaga
    38041 acgggcgcac tcaagcgcgc aaaggcgtgc atgctaccgt ctaggggaat cacggagtcg
    38101 tccgtgtggt atcgagtttc tcgtcggtct tgttcaggat cgacggtttt ggaatatcaa
    38161 cggcaatcac ttgatggagt actgacgtca tggctcagac aagaatttct tatcgaaaac
    38221 tcggacatag cgacttgaac gtgtctgcca tcggtttggg atgcatgtcg ctctcgggcg
    38281 tctacggagc ggctgacgac gccgtctccg aggcgctcat tcagcacgcg ctggatatgg
    38341 gcgtgaatca tctcgatact tcggacatgt atggctgggg ccacagcgaa caagtggtcg
    38401 gccgtgccat caaaggtcgc cgcgacaagg tgatgctggc ctccaagttc ggccaaatca
    38461 agcaagaggg cgcgggcaat ggcgtcaatg gtcgtccgga gtacgtccgc caggcctgcg
    38521 aggacagtct caagcgcctg ggcgtggatg tcatcgacct gtattaccag caccgcgtcg
    38581 acccggacgt gcccatcgag gacacggtcg gcgccatggc ggatctggtg cgtcagggca
    38641 aggtgcggtt tctgggtctc tcggaagcgc atgtcgacac gatacgcaag gcccacggtg
    38701 tccatccgat cacggcgttg cagtctgaat tctccattct gtaccgcgcc gaagccgaag
    38761 agacgcgcag ggtcacgaag gagctgggca tcagttttgt cgcctatgcc cccttggggc
    38821 gcgggctgtt gacgggcgaa attaaaaccc tggccgacgt cgatggccgc cgggcatcgc
    38881 atcctcgctt tcagcccgaa cactttgaac acaaccgcaa gctggccgag ccgatcgaag
    38941 tcctggcgaa agagaagggc tgcacgccgt cgcagatcgt attggcctgg ttgctggccc
    39001 aaggcgagga tgtggtgacc atcccgggga cgcgaaagac tgcgcgtctg gacgaaaacg
    39061 tcggggcatt ggatgtggtc ttgtcccagg ccgaaatcga cggcttgacc gccgccattc
    39121 cagccgacgc ggtgtcgggc atgcgttatc cggccgcgag tatggctcgg gtgaatctgt
    39181 aacggggatg gcgatcggta atcgctataa agagaaggat ctacgtctcg gcggcccgcg
    39241 tggtcttgtg tcctggcatt acgcctgggg ctactctcgt aaaatggccc gatgacaacg
    39301 atatccgaaa atccaaccat tgccctcgtc ggtccggggg caatcggcac gaccgtggcc
    39361 gcagcgcttc atgaagccgg acgcacgccg acgatttgcg gccgctcggc gcacgagcgc
    39421 ctggaactgc gcgtcgacgg tgagcgtatc ttcgtaccgg gccccgtaca gattgatccg
    39481 gccgagatca agaacccatt cgacctggtt tttgtcgcgg tcaagtccac gcaagtggaa
    39541 gccgtggcgc cctggctttc tgcgttgtgc agcgcgagga cggttgtctg cgtcctgcaa
    39601 aacggcgttg aacagaaagc gctctttgca ccgcacgtgc ccggcagtcc agtgctgccc
    39661 tcggtcgtat ggtttcctgc tcaacgagaa ccggacgcct cggtgtggct acgcggtaaa
    39721 gcgcgcatca ccttgcctga cacgccggaa gccagcctgg tgctcgcggc tttgcgcggc
    39781 acacggtgcg cggttgacgt agcggcggat ttcacctccg tcgcgtggcg caagcttctg
    39841 cagaatgcgg tcgcggggct catggttctg gcaggccgcc gtgcgggcat gttctcccgg
    39901 gacgacatca ccaggctgtc tcttgcctac ctccaggaat gtctcgaggt tgcgcgtgcc
    39961 gagggtgcgt cgctgggcga cggagtgccg caagagatca tcgatggatt tcaccgttcc
    40021 ccggccgact tgggcacgtc gatcctcgcc gaccgggaag ctggccgccc gcttgaatgg
    40081 gacagtcgga acggggttgt gcagcgccgt ggtcgatctc gcggcatccc gacgccaatt
    40141 agcgacctgg tcgtgccgct tttagcagcc gccagcgacg ggcccggcta ataacttcgc
    40201 tgatggaggt ctctcgagcg atggatcaga cctcacaaac agtctgcacg tccgaagatg
    40261 ctgctcatga ccttccagaa tctatcggtt gagatcttat ataaggatgc cccgcgtcgg
    40321 tgccgtctgt tcagtcgttc tcgaactgag gcggcgacaa atcccggagc atcctgacga
    40381 aggtctgagc gatgcggctc agtcgcacat ggcggcgccg tatgacgccc atcgagcgaa
    40441 acaccctcgg accatagatc ggcaagcagg ccagtccttc gatcgcgaga tccggaatga
    40501 tgctcgaaga caggaccgtg attcccatgc cttgccgaac catcgccaag gccgtcgcca
    40561 cttgctccac atcgcaggtc ggaatcaccg aaacgccttg ggctcgcatg gcgtcctccg
    40621 cctgccctga agacaccagg accaggggcc aatcgttcaa cgtccgccag ttgggtttgc
    40681 ttccaacgag cggatgtttg gcgggaacaa cgatcatgag gcggtcggtg tacagcgact
    40741 gaaagctcaa ttcgggatgc ggcccgctca aggtgcagat gccgaattcg acctcgcctt
    40801 tcaacacggc ttcgcgctgt tggtcatgcc gccgctcaac cagcgtgatg gtgacatccg
    40861 gatgcgcgcg ctggaattcg gcaagcttgc gagtgatggc gcccgaggcc atggagggta
    40921 atgcggccag acgcaggcgt ccgcctacac cgttgctgac gtcgcgtgcg ttgcggtagg
    40981 ccagttcgat ctcgcgcagc ggagatttga tttgctcgta gagaccctgg gcggcatcgg
    41041 tcggcacgca ccgccgggtg gtccgctcga acagcttggc ctggaggttg gattccaggt
    41101 cccggatcag cgccgaaaag gacggctgcg agatttccat tttctgcgcc gcgagggtga
    41161 aggaggtcga ctccatcgcc gtcacgaacg cgcgcatctg gcgaaacttg ggatcgggat
    41221 tcataggcat agccacataa taaagggtct taaccttatt gactcgataa gaccgatctc
    41281 tagaattgtc aagcactgat ctcctcgaaa ggtgcggaaa tgatgatgtt ggcaagtggc
    41341 ccttcagtgt ggcgtggcgt cgagatggcg caggacccgt cctgggtgtg gcagctgcct
    41401 gcgagcggcg tgaatacgat cgagcgcgcg ctggaaagcg tgatgcggcg caatatccag
    41461 gttcccgaga tgcgcaagga ggatttcgct ttgccggggc tggaagaaaa cttccatgca
    41521 atcgccggcg aggtcgatac cgggcgcggc tttcaggtcg tgcggggcgt gccggtcgcg
    41581 ggctatagcg aagatgaatt gcatcggctg acttgggggc tgggacttca cttcggcgtg
    41641 cccgtggagc agaccaagga cgccgattgg ctgatcgatg tccgcgacga gggtggcagc
    41701 tacgaccgca attcgcgggg ctaccacagc gcttccaagc tggaattcca taccgacggg
    41761 gccagcatcg tcggcttgct gtgcgtgcac aaggcagagg agggagggct cagtgtcctg
    41821 gtgagcgccg gcgcggtgca caacgaactg gccaggaccc acccggagca tttgattccc
    41881 ctctacgacg ggttccccgt ggatcgccgc ggggcgcagc cgccgggcga accgcgcgtg
    41941 tcgccttggt atctcccggt gttttcggtc acgcgcggcc agctcaattg cgtctatagc
    42001 cggggcgctt gcatctgggg gcgcgagcac ctcgggcaac cctgcagcga ggccgagatt
    42061 gccgcgctgg atgccttcga ggctatcgcg agcgatccgg gcttccggct cgacatggat
    42121 ttgcagccag gcgatctgca actggtcaac aactttactg tcttgcattc gcgtaccgcg
    42181 ttctacgaca acccggcttc gccgcagcgc cggcatcttg tgcgactgtg gctggacgtc
    42241 gaggggagcg accatgcggc gatcaacaaa cttcacctgt acacaaacaa gccgctaccc
    42301 agtttagtgg cggtgaggcc gcggacctga gaatctgggc acggcacaac tcaaatataa
    42361 ataaggagac aaaatggtga cgaaattaac gaaagccctc cgggcgcttg cgtcggcggt
    42421 cgcgatggca gccgtcgcgc cggcagcgct ggcacaggac gcgggcaact ggccggcgca
    42481 ccagccggtg aaaatcgtgg tcccctttcc accgggcggc gcagcggaca tcaacgcacg
    42541 gcttctggcc gccaagctgt ccgagatgaa ctcggcaaac ttctacgtcg agaaccatgc
    42601 cggcgcaaac gggatcatcg ggctggactt cgtcgccaag tcggcgccgg acggctacac
    42661 gctgctttcc agcaacctga gctcgcacgg cacgggacct gcggtctaca agacgctgcc
    42721 ctatgacccg atcaaggact tcatccccgt ggtgcaggtg accgcgacgc ccatgctgct
    42781 ggtcgaaagc cccaagctgc cctaccgcag cctgcaggag gtgatccagg cggcgaaagc
    42841 caatccggga aagataacca tcgcctcggc gggcaacggt tcgtccaccc atcttgccgc
    42901 tgaaatgctc aatgaaatgg cgggcatcaa gctgctgcac gttccctaca agggagacac
    42961 ccccgccatg gtcgacgtcg cgtccggcgt gtgcgacttg ctgctggtga gcacggaagc
    43021 gtccgaaccg ttcgtgaaaa gcgggaaact gagggtattg gccgtgggaa gcctgaagcg
    43081 cctgccgacg ctgcccgatg tgccgaccct ctccgaggcc ggggttccag gctatgtcgg
    43141 aagcacctgg agcggactgc tcgcgcctgc cgggacgccg gcagcgatcg tcgacaaact
    43201 caaccgccag gtaaaccaga tcgcccaaac gccggatttc cggcagcgcg tcgaatccgc
    43261 cggctcgcaa gtgatcggcg gcacgccggc caccttcggc acgctgatcc agtccgagat
    43321 tgccaagtgg acccgcgtcg cacatgacaa caacatcgtg ctgaacccgt gaccctgccg
    43381 cgggagatca ggccggccac ggcagcctgg acgacaggcc ggccaggtcg ttgaccggtc
    43441 ccaacacggt atcgaaccgc atggtggtgc gatggcgcct gtcataaggc gcccaacgcg
    43501 gcaggccatc gtggttcggg ttgcccgtgc gcgcgaaggc gatccatgcc tgttgcatgg
    43561 cgtgcgcaag gccgtcgaat tcggccgggt ccacgccttt gagcatcggc gcgtccggcc
    43621 agttctcgat gttgtggaag acgaagggga tttccaggca atggcaggcc tggaagcccg
    43681 ccggcgactg ccagtcgaac tggtacacat acgcggggcg gccatggtcc gccagcattt
    43741 ccgccgtctg caaggtgccg atgcggaaaa cgtagtcggt caacaggtcg cccagcatcg
    43801 aggccgcggt gggcttggcg cgcaggcgcc ggaattcgtc gtagtacgcc tcgtaatcgg
    43861 ggccgcacag tttttcgaat acctttttca cagcctggat gtcggcggcc gcgatggcgg
    43921 ggtcggtgct gaagaaggcc gcgcgctctt ccctcagggt gcccagcatg atgtcgacgg
    43981 gagcgccgcg tcccgcgcgc aggcagccgg tgatgtcctc ggggatggcg ttgccgtcct
    44041 gcacgagggt gaagggcagc atcatggtcg cgaacgattg caggctcttg gccagttcgc
    44101 cctgggcttt gagcaggcgg tcgaccggca cctgcttcag gccggcgata tcgtcgcgcg
    44161 ccaggcccag cagatccata tacgttgcgc cctgggcggc ggcctcggcc acgggccggg
    44221 tgggccggct catgcccgga ctttgcagga tcgcgcgctg gaacagtccc tttgccgtgg
    44281 gcatggtcaa catcgcggcg atggtgttgc cgccgacgga ttggcccacc aaggtcacgt
    44341 tgtccggatc gccgccgaaa ttcccgatgt tttcccgcac ccagcgcagc gcttgcacct
    44401 ggtccagcaa acccaggttg ccagggctga cgcccggcaa atacaggaag cccagcgcgc
    44461 ccaggcgata attgaccgac accaccacga catcgccatt ggccgccatg cggtcgccgg
    44521 cataccaggg cagcgaaccc gcgccgctgg aataggcgcc gccgtgcagc cacacgatga
    44581 ccggccgcgc acccttgccg atgccgggcg tccagacatt cagcgtcagg cagtcttcgc
    44641 tttgcggacg ctggaattcg cccatgacat gagccagccg cgagcgtccc tgcggcgcga
    44701 tcgggccgtc cgcggtggcg tcgcgcacgc cctgccatgg cgccggcggc cgtggcgcct
    44761 cgaagcgcag cgcgcccacg gggggagcgg catagggaat gccgcgaaac accgcgatgc
    44821 cgttctcgat gcgcccgcgc agcgcgccag ccgtggtgcc gacgtcttgc gtggcttgcg
    44881 ttgccgtatt cattttcgct atcctttctt tcgagcctgt gcggcccttg tcctgtcaac
    44941 gcgccgcgaa cctgcgttcc agaggcacga tgaccagccg ctc
//
LOCUS       DASWPO010000279         4854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3193227, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000279 DASWPO010000000
VERSION     DASWPO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3193227"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1006
                     /locus_tag="VGC69_14765"
     CDS             86..1006
                     /locus_tag="VGC69_14765"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY0296588.1"
                     /translation="MSYRIKGVLSPVVTPFDKDLGVDLALFTAQCRWLQANHVGLAFL
                     GTNSEANSIGPDERMDMIDALIQAKLDPRGMMPGTGACDLPTVVRLTQRAVQSGCAGV
                     LMLPPFYYKGVSDEGLYRYYSEVIQRVGDERLRIYLYHIPPVAQVPISLGLIDRLLRA
                     YPGIVAGVKDSSGDWSNTQAMLERFAPLGFDVFAGSETFLLQTLRHGGAGCISATANV
                     NAAAIHALYRGWQEPGADARQQELDVVRQIFQGFPMIPALKAAIAHWSRIDAWQRVRP
                     PLVELDDGQHAKLIRQLEAVGFSIAGLQAA"
     gene            complement(1023..1658)
                     /locus_tag="VGC69_14770"
     CDS             complement(1023..1658)
                     /locus_tag="VGC69_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY0296589.1"
                     /translation="MDTPAKKPKLSETFIAQTVSTRQTEVGKCCEILDRTRLEYTTLG
                     DYSYLDRDCMVADAEIGRFCAIAACVRIGAPNHPFRRASQHRFTYCPEYYDAAATRDA
                     GFFAERRDDKVIIGNDVWIGHGVTVLPGVRVGNGAILAAGAVVAKDVAPYEIVGGVPA
                     RRIRERFPAAIAERLQRIAWWNWPFDALMENLADFQSEDMEQFCEKWDRAA"
     gene            1841..3127
                     /locus_tag="VGC69_14775"
     CDS             1841..3127
                     /locus_tag="VGC69_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0296590.1"
                     /translation="MSSKKKTACWAVGAVVVAGAAAAFAYAWQPSIEPLAQAPVPSAN
                     QTLLAQGASLAELGDCMQCHTAKDGKPYAGGAPLATPFGTIYTTNITPDVDTGIGSWT
                     LEAFTRALRKGVSRDGHLLYPAFPYPHFTRMSDSDIAALYAYLMARTPVHAPAAANDL
                     TFPLNFRPLVAGWNLLFLRQGPLPQPALAQSDAWLRGRYLVEGAGHCASCHTPMNALG
                     AEKLDQPYAGGLIDGWNVPPLNALTLARKPWTEDQITDYLRTGIASEHSAAAGPMLPV
                     AHHLGNAPESDVRAIATYLMSLQPPMPAQPAAPAPVATVDSQPLNPGAALFNASCAGC
                     HSAAAPMRSLGDRPALELGSTINADSPRNAIQMVLNGNPWNGSSSAHYMPPFASVLTD
                     QQIADVLAYARSTYAQRPAWSGLPASVAAIRKENSK"
     gene            3127..3651
                     /locus_tag="VGC69_14780"
     CDS             3127..3651
                     /locus_tag="VGC69_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0296591.1"
                     /translation="MVTLTVNGVDHDLDIDPSMPLLYALRNHLQLNGAKYGCGLGQCG
                     ACTVIVDGKPVFSCLTPVSAVGKRPVRTIEGLGTPEKPSVLQKAFIDKQAAQCGYCIA
                     GMIMRAQALLDMHPGATETEIREHMQPNLCRCGTHMRIISAIVAASQSLRSPQAENGA
                     APQATAAIESGDPA"
     gene            3648..>4854
                     /locus_tag="VGC69_14785"
     CDS             3648..>4854
                     /locus_tag="VGC69_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0296592.1"
                     /translation="MNKPLDIPPHDLVDHGRRRFLAAGSLTVSFALMPGASVLAQQRP
                     TEDAAPTAEAGTAALPGNLSSTPLLDAWIRIDEQGKITVYTGKAELGTGIRTAFIQIA
                     AEELQVAPSAINLITADTGLTPNEGYTAGSHSTADSGTAIYNAAAQVRALLLQSAAEK
                     LGLLADTLQAGNGVISAPDGRTLNYGQAVSGLSLHRNAAPTSPLLDPKKHKVIGTPVQ
                     RVDIPGKVTGGASYVQDMRLPGMVHARAVRAPSYDARLVKADIEGAEKMPGVIKVVRD
                     GSYLAVVAEDEWQAIRAMRALMASAVWEETAKLPTPETIYDVLLKLPAREINSGNRHG
                     AAEPAVKSLHARFTKPYLSHGSIGPSCAIAHMDGGKLTVWTHAQGVFPLRGGLVELLG
                     MKPEDVRCIH"
BASE COUNT          827 a         1818 c         1476 g          733 t
ORIGIN      
        1 ctggctggcg tccctgaacc cgggcgcatg acccggtgat gcgcgccccc cttaaccgaa
       61 gctcccaagc gctataggaa tcgctatgtc atatcgaatc aaaggtgtcc tgtcgcccgt
      121 cgtcacgccg ttcgacaagg acttgggcgt cgacctcgcg ctcttcacgg cccagtgccg
      181 atggctgcag gccaaccacg tcggcctggc cttcctgggc acgaattccg aggccaactc
      241 catcgggccc gacgagcgca tggacatgat agacgccctg atccaggcca agctggaccc
      301 ccggggcatg atgcccggca ccggcgcctg cgacctgccc accgtcgtcc ggctcaccca
      361 gcgcgccgtc caatcgggct gcgccggcgt gctcatgctg ccgcctttct actacaaggg
      421 cgtcagcgac gaaggcctgt accgctatta ctcggaagtc atccagcgcg tcggcgacga
      481 gcgcctgcgc atctatctct accacatacc gccggtcgcc caggttccga tcagccttgg
      541 cctgatcgac cgcttgctgc gcgcctatcc aggcatcgtg gcgggcgtca aggattcgtc
      601 cggcgattgg tccaataccc aggccatgct cgagcgtttc gcgcccctgg gctttgacgt
      661 gttcgcgggc agcgagacct tcctgctgca gacgctgcgc catggcggcg cgggctgcat
      721 cagcgccacg gccaacgtga acgccgccgc catccacgcc ttgtaccgcg gctggcagga
      781 gcccggcgcg gacgcgcgcc agcaggaact ggacgtcgtg cgccagatat tccagggctt
      841 tccgatgatc ccggccctga aggcggccat cgcgcactgg agccgcatcg acgcctggca
      901 gcgggtgagg ccgccgctgg tcgaactgga cgacgggcag cacgccaagc tgatccggca
      961 gctggaagcg gtgggcttca gcattgccgg actgcaagcc gcctgaatcc ccgcgggttc
     1021 gatcaggcgg ccctatccca cttttcgcaa aactgctcca tatcctcgga ttggaagtcc
     1081 gccaggtttt ccatcagggc gtcgaacggc cagttccacc aggcgatgcg ttgcaggcgt
     1141 tcggcaatgg cggcgggaaa ccgttcgcga atgcggcggg cgggcacgcc gcccacgatc
     1201 tcatacgggg cgacgtcttt ggcaacgacc gcgcccgccg ccagaatggc gccgttgccc
     1261 acccgcacgc caggcagcac cgtaacgccg tggcctatcc agacatcgtt gccgatgatg
     1321 accttgtcgt cgcgccgctc ggcaaagaat ccggcgtccc gcgtggcggc ggcgtcgtag
     1381 tattcggggc agtaggtgaa ccggtgctgg ctcgcgcgcc ggaatggatg atttggcgcg
     1441 ccgatgcgca cgcaggcggc aatcgcgcag aacctgccga tctcggcatc ggccaccatg
     1501 cagtcgcgat ccaggtagct atagtcgccg agcgtggtgt attcgagccg ggttctatcc
     1561 aggatctcgc agcatttgcc cacctcggtt tgccgcgtgg atacggtctg ggcaatgaag
     1621 gtttccgaca atttgggctt tttggccggc gtatccatgg tctgattcgc gtcacatgca
     1681 aaggctggga tgctagccgc gcctccctac agaaatatgt cggttttccc tgatttgtat
     1741 tgctgtctcg cggcacaatc cgcctgaagt tcagcctggg ttcagtacaa cttattagca
     1801 tacgcatcca ctcgcgtaca caaatccgcg tggaggattc atgagcagta aaaagaaaac
     1861 cgcctgctgg gcggttggcg ccgttgtcgt cgccggcgcc gccgcggcct ttgcctatgc
     1921 ctggcagccg tccatcgagc ccctggccca ggcccccgtc cccagcgcca accagaccct
     1981 gcttgcccag ggcgccagct tggcggaact gggcgactgc atgcagtgcc atacggccaa
     2041 ggacggcaag ccctatgccg gcggggcgcc cctggcgacg ccgttcggca ccatttacac
     2101 caccaacatc acgcccgacg tcgacaccgg catcggcagc tggacgctgg aagccttcac
     2161 ccgcgccctg cgcaagggcg tctcgcgcga cggccacctg ctgtacccgg cctttcccta
     2221 cccgcacttc acccgcatgt cggacagcga catcgccgcg ctgtacgcct acctgatggc
     2281 gcgcacgccg gtgcacgccc cggccgcggc caacgacctg accttcccgc tgaacttccg
     2341 gccgctggtg gcgggctgga atctcctctt cctgcgccag ggtccgctgc cccagccggc
     2401 cctggcgcaa tccgacgcct ggctgcgcgg ccgctacctg gtcgaagggg cgggccactg
     2461 cgcatcgtgc cacacgccga tgaacgcgct cggcgccgaa aagctcgacc agccctacgc
     2521 cggcggcctg atcgacggct ggaacgtgcc gccgctcaat gccctgaccc tggctcgcaa
     2581 gccctggacc gaagaccaga tcaccgacta cctgcgcacc ggcatcgcca gcgagcacag
     2641 cgccgcggcc ggccccatgc tgccggtcgc gcaccacctg ggcaacgcac ccgagtccga
     2701 cgtgcgcgcc atcgcgacct acctgatgtc gctgcagccg cccatgccgg cccagccggc
     2761 cgcgccggcg cccgtggcga cggtcgactc gcagccgctc aacccgggcg cggcgctctt
     2821 caacgcatcc tgcgccggct gccacagcgc cgccgcgccg atgcgcagcc tgggcgaccg
     2881 cccggccctg gaactgggca gcacgatcaa tgccgacagt ccgcgcaacg ccatccagat
     2941 ggtgctcaac ggcaacccct ggaacggctc ttcgtccgcg cactacatgc cgccgttcgc
     3001 cagcgtgctg accgatcaac aaattgccga cgtgctggcc tacgcccgct cgacctacgc
     3061 gcagcgcccg gcctggtccg gcctgcccgc cagcgtcgcc gccatccgca aggagaactc
     3121 gaaataatgg ttacgctcac cgtcaacggg gttgaccacg acctcgacat cgatccttcc
     3181 atgcccttgc tgtatgcgct gcgcaaccac ctgcagctca atggcgccaa gtacggctgc
     3241 ggcctcggcc aatgcggcgc atgcaccgtc atcgtcgacg gcaagcccgt gttctcctgc
     3301 ctgacgcccg tatcggccgt cggcaagcgg cccgtgcgga ccatcgaagg cctgggcacg
     3361 ccggaaaagc cctccgtcct gcaaaaggcc ttcatcgaca agcaagccgc ccaatgcggc
     3421 tactgcatcg caggcatgat catgcgcgcc caggcgctgc tcgacatgca ccccggcgcg
     3481 accgagaccg agatccgcga gcacatgcag cccaatctgt gccgctgcgg cacgcacatg
     3541 cgcatcatct ccgccatcgt ggcggcctcg caaagcctgc gcagcccgca ggcggaaaac
     3601 ggcgcggcgc cccaggcgac cgccgccatc gaatcgggag acccggcatg aacaagcctc
     3661 tcgacatccc gccgcacgat ctcgtcgacc acggccgccg ccgcttcctc gccgccggtt
     3721 cgctgaccgt cagcttcgcc ctgatgcccg gcgcgagcgt gctggcccag cagcgcccga
     3781 ccgaagacgc cgcccccacc gcggaggccg gcacggccgc gctgcccggc aacctgagca
     3841 gcacgccgct gctggatgcg tggatccgca tcgacgagca aggcaagatc acggtctaca
     3901 ccggcaaggc cgaactgggc acgggcatcc gcacggcctt catccagatc gccgccgaag
     3961 agctgcaagt cgcgccctcg gccatcaacc tcatcactgc cgacaccggc ctgacgccca
     4021 acgaaggcta taccgccggc agccactcca ccgccgacag cggcaccgcc atctacaacg
     4081 cggccgccca ggtgcgcgcg ctgctgctgc agtcggccgc cgagaagctc gggctgctgg
     4141 ccgacacgct gcaggccggc aacggcgtca tcagcgcgcc ggacggacgc acgctgaact
     4201 acggccaggc cgtcagcggc ctgagcctgc atcgcaatgc cgcgcccacc tcgccgctgc
     4261 tcgaccccaa gaagcacaag gtcatcggca cgccggtgca gcgggtcgac atccccggca
     4321 aagtcaccgg cggcgccagc tacgtgcagg acatgcgcct gcccggcatg gtgcacgccc
     4381 gcgccgtgcg cgcgccctcc tacgacgcca ggctcgtcaa ggccgacatc gaaggcgccg
     4441 agaaaatgcc cggcgtcatc aaggtcgtgc gcgacggcag ttacctggcc gtggtcgccg
     4501 aggacgaatg gcaggccatc cgcgccatgc gcgccctgat ggcctccgcc gtctgggaag
     4561 aaaccgccaa gctgcccacg cccgaaacca tctacgacgt gctgctcaaa ctgcctgccc
     4621 gggaaatcaa ctcgggcaac cgccacggcg cggccgagcc ggccgtcaag tcgctgcacg
     4681 cgcgcttcac caagccctac ctgtcgcacg gctcgatcgg cccgtcctgc gccatcgccc
     4741 acatggacgg cggcaagctc accgtctgga cccacgcgca aggcgtgttc ccgctgcgcg
     4801 gcggcctggt cgagctgctg ggcatgaagc ccgaggacgt ccgctgcatc cacg
//
LOCUS       DASWPO010000280         8512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_870655, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000280 DASWPO010000000
VERSION     DASWPO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870655"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VGC69_14790"
     CDS             <1..1027
                     /locus_tag="VGC69_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HEY0296593.1"
                     /translation="PHCHITPAMRGGADFLVAFKEYPHTDVVERGLDLVDLCVAKAQG
                     KIQPAHAIVDTGMIATIRTPQEPARGFVDRIKAMEGKDGVLSISIGHGFPWGDVADMG
                     TRVLVYTDNDPAKAQALARRLADELIALRDALDTPMPGIEEALDQAMAMDGTVILADS
                     ADNPGGGAAGDSTFILRRLVERGIAPAAVGPVWDPVAVRIACDAGEGATLDLRIGGKI
                     SPASGDPMDLRCTVKAIRPGMTMTGLTGTPMAMGDCVLVEARGIEIVLASIRNQAMGT
                     DVFTKLGCDLAAKKLIVVKSSQHFYASFAKVSTNVVYVAAPGAVTRDIATLPYRNIRR
                     PKWPLDA"
     gene            1077..2684
                     /locus_tag="VGC69_14795"
     CDS             1077..2684
                     /locus_tag="VGC69_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296594.1"
                     /translation="MKRPTLAARALRCLAAAATAAAILTAGAPAQAQPAPVKTLRMVA
                     QADLKILDPTFTTAYITRNFGYMVYDTLFGMDSKGVPQPQMVGKYEKSADGLKWSFTL
                     RPGLKFSDGAPVTTADVLASIQRWTAHDGTGRLMTAAGAQWAAADAQTFTLTLTQPFG
                     LVLDALAKPSSFPLMIMPERLAKAPANAPLTEVLGSGPFMFKRDEWVPGNKVVFVKNP
                     YYVPRSEPADGLAGGKKVDFDRMEWLYLPDANSAVAALKNNEVDYIEQVPPDYIAPLR
                     AESDIKVGSASIYQGWIIMNQLHPPFDNPRIRHALLEAVSQEQFMAGMGYPADMTMKF
                     CPGYFICGGSVFSSAGGEAYRTPDLEKAKKDLIDAGYKGEKVVLLVPSDVTYLNAEAL
                     MAAQTMQSIGMNVDMQTMDWASIGARRAKRDAPDAGGWNVYVTVAGRFDADTPVTHAL
                     LSAQCGNNLPGWFCDKTLDDLRAAWIRESDPAKRQAILDRFQARAYEVAPSINTGQYT
                     TAYAVRNTVKGTEKFWGGLPLVWMLDK"
     gene            complement(2713..3597)
                     /locus_tag="VGC69_14800"
     CDS             complement(2713..3597)
                     /locus_tag="VGC69_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEY0296595.1"
                     /translation="MPTVPLRFGLTANRLHHETPDAALFTWLRACSRTIRELGIELHT
                     VGRAYDAILREGMLQGYPGLVRYPYGRDGGLMKVVARVTPPRAGQASLDGAIYLIDPV
                     DPSSIFPEMLALKRQCITHGLPFISTVAGAIEWMEIERVRAGMAPDPTADDLFEFGSQ
                     TLALIAHDALKPEMVAFADQHFDLLSRFAQRVATGTTGALLNELAWSRGWPRDTPWVQ
                     RYLSGPLGGDAQIAELVLERHCRRVIFFEDPHVARQHEADIQLLERAVRVKTDYAMCA
                     TSPTVARRWAGVAEKRGV"
     gene            3889..4065
                     /locus_tag="VGC69_14805"
     CDS             3889..4065
                     /locus_tag="VGC69_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY0296596.1"
                     /translation="MKSNEFKRWLARQGATFGPMRGSHLKVYLNGRQSILPMHNTELK
                     TGTVEGIKKQLGLK"
     gene            4078..4506
                     /locus_tag="VGC69_14810"
     CDS             4078..4506
                     /locus_tag="VGC69_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0296597.1"
                     /translation="MMRYPVTLTPDKTSGGFVVTFPDIPEAITQGETTEEAVAMAQEA
                     LETAMEFYFEDKRIVPAPSKPKRNQPVVELPASLTAKVLLLNEMITQNVRPAELARRL
                     HTTPQEVNRLTNLHHTTRIDSIESALRALGRHLEISLSAH"
     gene            complement(4547..4741)
                     /gene="iscX"
                     /locus_tag="VGC69_14815"
     CDS             complement(4547..4741)
                     /gene="iscX"
                     /locus_tag="VGC69_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEY0296598.1"
                     /translation="MKWTDVYEIATALADAHPAIDPKTVRFTQLRDWVMALPGFSDDP
                     AHCGEKVLEAIQMAWIEEAD"
     gene            complement(4741..5082)
                     /gene="fdx"
                     /locus_tag="VGC69_14820"
     CDS             complement(4741..5082)
                     /gene="fdx"
                     /locus_tag="VGC69_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEY0296599.1"
                     /translation="MPSLTVLPHPDLCPDGALIADAPQGVSICRVLLDNRIEIEHACE
                     LSCACTTCHVIVRQGYNSLDEASESEEDMLDKAWGLSSTSRLSCQAKVGTQDLTVEIP
                     KYSINHAKEDH"
     gene            complement(5123..6988)
                     /gene="hscA"
                     /locus_tag="VGC69_14825"
     CDS             complement(5123..6988)
                     /gene="hscA"
                     /locus_tag="VGC69_14825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEY0296600.1"
                     /translation="MALLQISEPGESPAPHQRRLAVGIDLGTTNSLVAAVRSSAAEVL
                     PDDEGRRLLPSAVQYLPDGRAPTGHAALAQQAADPRNTIVSVKRFMGRSLAEAQASGA
                     PYEFVDAPGMVRLRTVQGDLSPVEVSAQILAVLRQRAEDVLGDDLVGAVITVPAYFDD
                     AQRQATRDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGVYAVYDLGGGTFDISILRL
                     SRGVFEVIATGGDTALGGDDFDAVIVQHVLENLPAGGDLTPGDRRGLLVAARTAREAL
                     SDQEAVRFALTLADGRQVARDITRGDFESWARPLVERTLDSARRALRDAKLSTADVRG
                     VVMVGGSTRMPVIRRAVGELFGTEPLVNLNPDEVVALGAAQQANLLAGNRAPGEDWLL
                     LDVIPLSLGLETMGGLVERVIPRNSTIPVARAQEFTTFKDGQTAMSVHVVQGERELVS
                     DCRSLARFELRGIPPMVAGAARIRVTFQVDADGLLSVTAREQSTGVEAAVTVKPSYGL
                     SDEEIARMLSDSMLEADSDARARMLREQQVEASRLAESVRAALAGDSDLLSADERAAV
                     DAALQGAQAVAQSDDVDAVRAAVQTLSSVTEDFAARRMDRSIRKALAGRKLDELA"
     gene            complement(7048..7557)
                     /gene="hscB"
                     /locus_tag="VGC69_14830"
     CDS             complement(7048..7557)
                     /gene="hscB"
                     /locus_tag="VGC69_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEY0296601.1"
                     /translation="MAADDYFSLFGLPARFELDQNALEHAWRDVAARVHPDRFATASA
                     AERRVAMQWASRANEAYRQLRDPLLRARYLCEQAGVDLQTESNTAMDPAFLMQQMEWR
                     EMLDDARRDPQVVPVLQAELEAAQAGMRRQVGALLDGQGDAIGASVRVREWMFLDRLA
                     QELAALRPI"
     gene            complement(7565..7888)
                     /gene="iscA"
                     /locus_tag="VGC69_14835"
     CDS             complement(7565..7888)
                     /gene="iscA"
                     /locus_tag="VGC69_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY0296602.1"
                     /translation="MAITLTQQAANHVTRYLAKRGKGVGLRLGVRTTGCSGMAYKLEY
                     VDAPDTADEVFESFGVKVFVDTKSLPYIDGTELDYAREGLNEGFKFRNPNEKATCGCG
                     ESFTV"
     gene            complement(7890..8303)
                     /gene="iscU"
                     /locus_tag="VGC69_14840"
     CDS             complement(7890..8303)
                     /gene="iscU"
                     /locus_tag="VGC69_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEY0296603.1"
                     /translation="MSYSNKVLDHYENPRNVGSFDKGDESIGTGMVGAPACGDVMKLQ
                     IKVGADGVIEDARFKTYGCGSAIASSSLVTEWVKGKTLDQAMSIRNTQIAEELALPPV
                     KIHCSILAEDAIKAAVSDYRAKHGEAVQAEAAAVN"
BASE COUNT         1475 a         2959 c         2711 g         1367 t
ORIGIN      
        1 cccgcactgc cacatcaccc cggccatgcg cggcggcgcc gacttcctcg tcgccttcaa
       61 ggaatatccg cataccgacg tggtcgagcg cggcctcgac ctggtcgacc tgtgcgtggc
      121 caaggcgcag ggcaagatcc agcccgcgca cgccatcgtc gataccggca tgatcgcgac
      181 catccgcacg ccgcaggagc ccgcgcgcgg tttcgtcgac cgcatcaagg ccatggaagg
      241 caaggacggc gtgctgtcga tctccatcgg ccatggcttc ccatggggcg acgtggccga
      301 catgggcacg cgcgtgctgg tgtacaccga caacgatccg gccaaagcgc aggcgctcgc
      361 gcgccggctg gccgacgaac tgatcgcctt gcgcgacgcg ctcgacacgc ccatgcccgg
      421 catcgaggaa gcgctcgacc aggccatggc catggacggc accgtcatcc tggccgacag
      481 cgccgacaac cccggcggcg gcgcggccgg cgactccact ttcatcctgc ggcggctggt
      541 cgagcgcggc atcgccccgg cggcggtcgg ccccgtgtgg gaccccgtcg cggtgcgcat
      601 cgcctgcgac gccggcgaag gcgccacgct ggacctgcgc atcggcggca agatcagccc
      661 ggcctcgggc gaccccatgg acctgcgctg caccgtcaag gccattcgcc ccggcatgac
      721 catgaccggc ctgaccggca cgcccatggc catgggcgac tgcgtgctgg tcgaggcccg
      781 gggcatcgag atcgtgctgg ccagcatccg caaccaggcc atgggcaccg acgtcttcac
      841 caagctgggc tgcgacctgg ccgccaagaa gctcatcgtg gtgaagtctt cgcagcattt
      901 ctatgcctcg ttcgccaagg taagcacgaa cgtggtctac gtagcggcgc caggcgccgt
      961 gacgcgcgac atcgccaccc tgccttaccg caacatccgg cgccccaagt ggccgctcga
     1021 tgcctgaacc acgacagcgg ctgcctgcgc atcccgattc ccatggagct tgcacgatga
     1081 aacgaccaac cctcgcggca cgcgcgctgc gctgcctggc cgccgcggcc acggctgcgg
     1141 ccatcctcac ggccggcgcg cccgcccagg cgcagcccgc gcccgtcaag acgctgcgca
     1201 tggtggcgca ggccgacctg aagatcctcg acccgacctt caccacggcc tacatcaccc
     1261 gcaacttcgg ctacatggtc tacgacacgc tgttcggcat ggacagcaag ggcgtgccgc
     1321 agccgcagat ggtcggcaag tatgaaaagt cggccgacgg cctgaaatgg tccttcaccc
     1381 tgcgccccgg cctgaagttc tccgacggcg cgcccgtcac cacggccgac gtgctggctt
     1441 ccatccagcg ctggaccgcg cacgacggca cgggccgcct gatgactgcc gccggcgcgc
     1501 aatgggcggc cgccgatgcg cagaccttca cgctcacgct cacgcagccc ttcggcctgg
     1561 tgctcgatgc gctggccaag ccctcgagct ttccgctgat gatcatgccc gagcgcctgg
     1621 ccaaggcccc ggccaacgcc ccgctcaccg aggtgctggg cagcggcccc ttcatgttca
     1681 agcgcgacga atgggtgccc ggcaacaagg tcgtcttcgt caagaacccc tattacgtgc
     1741 cgcgcagcga gccggccgac ggcctggccg gcggcaagaa ggtcgacttc gaccgcatgg
     1801 aatggctgta cctgcccgat gccaacagcg ccgtggccgc gctgaaaaac aacgaggtcg
     1861 actacatcga gcaggtgccg cccgactata tcgcgccgct gcgcgccgaa tccgacatca
     1921 aggtgggctc ggccagcatc taccagggct ggatcatcat gaaccagctt catccgccct
     1981 tcgacaaccc caggatccgc cacgcgctgc tggaggccgt cagccaggag caattcatgg
     2041 ccggcatggg ctatcccgcc gacatgacga tgaaattctg ccccggctac ttcatctgcg
     2101 gcggctcggt cttcagcagt gccggcggcg aggcctatcg cacgcccgat ctcgaaaagg
     2161 ccaagaaaga cctgatcgac gccggctaca agggcgagaa agtagtcctg ctggtcccca
     2221 gcgacgtcac ctacctcaat gccgaggcgc tgatggccgc gcagaccatg caaagcatcg
     2281 gcatgaacgt cgacatgcag accatggact gggcctccat cggcgcgcgc cgcgccaagc
     2341 gcgatgcgcc cgatgccggc ggctggaacg tctacgtgac cgtggccggc cgtttcgacg
     2401 ccgacacgcc cgtcacccat gccctgctga gcgcgcaatg cggcaacaac ctgccgggct
     2461 ggttctgcga caagacgctc gacgatctgc gcgccgcctg gatccgggaa agcgacccgg
     2521 ccaagcgcca ggccatactc gaccggttcc aggcccgcgc ctacgaggtc gcgccctcga
     2581 tcaacaccgg ccaatacacc acggcctacg ccgtgcgcaa cacggtcaag ggcaccgaga
     2641 agttctgggg cggcctgccg ctggtatgga tgctggacaa atgatcctgg ctccgacccg
     2701 cggataggct tttcacacgc cgcgcttttc cgccacgccg gcccagcggc gcgccaccgt
     2761 gggcgaggtg gcgcacatgg cgtaatcggt cttcacccgc acggcgcgtt ccagcagctg
     2821 gatatcggcc tcgtgctggc gcgccacgtg cggatcctcg aagaagatca cgcgccggca
     2881 gtggcgctcc agcaccagct cggcgatctg cgcatcgccg cccagcgggc cgctcaggta
     2941 gcgctgcacc cagggcgtgt cccggggcca gcccctggac caggccagct cgttgagcag
     3001 cgcgcccgtc gtgcccgtgg ccacgcgctg cgcaaagcgc gagagcaggt cgaagtgctg
     3061 gtcggcaaag gccaccatct cgggcttgag ggcatcgtgg gcgatcaggg ccagcgtctg
     3121 gctgccgaac tcgaacaggt cgtccgccgt cgggtccggc gccatgccgg ctctcacccg
     3181 ctcgatctcc atccattcga tggcgcccgc caccgtcgag atgaaaggca gcccgtgcgt
     3241 gatgcactgg cgcttgagcg ccagcatctc gggaaagatc gaggagggat cgaccggatc
     3301 gatgagatag atggcgccgt cgagcgacgc ctgcccggcg cgcggcggcg tcacccgggc
     3361 caccaccttc atcaagccgc cgtcgcggcc gtagggatag cgcaccaggc cgggatagcc
     3421 ctgcagcatg ccctcgcgca ggatggcgtc ataggcgcgg ccgaccgtgt gcagctcgat
     3481 gcccagttcg cgtatcgtcc tggaacaggc ccgcagccag gtaaacagcg ccgcgtcggg
     3541 cgtttcgtgg tgcaggcggt tggccgtgag gccgaagcgc aaaggaacag tgggcatgac
     3601 tcgcttcggc gatgaaataa gccggcgcaa cgcttcggct cggggtcctg cataggcccg
     3661 ttcatcatac tgccttctct atggcaaagc cgtgccagag catcggtcaa tacgtgtctg
     3721 cggctagctg cgatgcagac cagcgagaca ctttggacat cgtgtgctct ggcatcgcat
     3781 cgcctggatg attagcatcg atctccttgc ccgaggcaag aatccgagac ctatcgaatc
     3841 tccttcgcaa tcctcttgtt aaataaacaa ttttgtttat aattttcagt gaaaagcaac
     3901 gagttcaagc gctggctggc ccggcaaggc gcgacgttcg gcccgatgcg gggctcgcac
     3961 ttgaaggtct atctcaatgg taggcaaagc atcctgccca tgcacaatac cgaactgaaa
     4021 accggtacgg tcgaaggcat caaaaaacaa cttggcctga aataaatgca gcggaatatc
     4081 atgcgctatc cagtcacgct caccccggac aaaacttcag gaggcttcgt cgtcaccttc
     4141 cccgacattc ccgaagcgat cacgcaagga gaaacgacgg aagaagccgt ggcaatggcg
     4201 caggaagccc tggaaaccgc catggagttt tatttcgagg acaaacgcat tgtccccgca
     4261 ccgtccaagc ccaagcgtaa ccagcctgtc gtggaactac ccgccagcct gacggcaaaa
     4321 gtgctgctgc tcaatgaaat gatcacgcaa aacgtgcgcc ctgccgaact ggcgcgtcgg
     4381 ctgcacacca cgccgcaaga ggtaaaccgg cttaccaacc tgcatcacac aacccgcatc
     4441 gacagcatcg aatcagcgct gcgcgccctg ggacgtcatc ttgaaatcag tctctcggcg
     4501 cactgaatta gccaaagcgg gacggagcgg gcttgaggca agccaatcaa tcagcttctt
     4561 cgatccaggc catctggatg gcctcgagca ccttctcgcc acagtgcgcc ggatcgtcgc
     4621 taaagcccgg cagggccatc acccagtcgc gcagctgggt aaagcgcacg gtcttggggt
     4681 cgatcgccgg gtgggcgtcg gccagcgccg tggcgatctc gtagacatcg gtccacttca
     4741 tcagtgatct tccttggcgt ggttgatgct gtacttgggg atctcgacgg tgaggtcctg
     4801 cgtgccgacc ttggcctggc acgacaggcg cgaggtggac gacaggcccc aggccttgtc
     4861 cagcatgtcc tcttccgact cgctggcctc gtccagcgag ttgtagccct ggcgcacgat
     4921 cacatggcag gtggtgcagg cgcaggacaa ctcgcaggca tgttcgatct cgatgcggtt
     4981 gtcgagcagc acgcggcaaa tcgagacgcc ttgcggcgca tcggcaatga gcgcgccatc
     5041 gggacaaaga tcgggatggg gcagtacggt caggctgggc atggtctgcg gtgctttctt
     5101 gaaaacaagg tagtcgttcg aatcaggcta gctcgtcgag tttacggccg gccagcgcct
     5161 tgcggatgct gcggtccatg cggcgcgcag cgaagtcctc ggtgaccgac gacaaggtct
     5221 gcaccgcggc gcgcacggcg tccacatcgt cggactgggc cacggcttgc gcgccttgca
     5281 gcgcggcgtc caccgcggcg cgctcgtccg ccgacagcag gtcgctgtcg ccggccaggg
     5341 cggcgcgcac cgactcggcc agccggctgg cctcgacctg ctgctcgcgc agcatgcggg
     5401 cccgtgcatc gctgtcggcc tcgagcatgc tgtcggacaa catgcgggcg atttcctcgt
     5461 cggacaggcc gtacgaaggc ttgacggtca cggcggcctc gacacccgtg ctctgctcgc
     5521 gcgccgtcac gctcaagagg ccgtcggcgt ccacctggaa agtcacgcgg atgcgcgccg
     5581 cgcccgccac catgggcggg atgccgcgca gctcgaagcg cgccagcgac cggcagtcgg
     5641 acaccagctc gcgctcgcct tgcaccacgt gcacgctcat cgcggtctgg ccgtccttga
     5701 aggtggtgaa ttcctgcgcc cgcgccacgg ggatggtgct gttgcgcggg atcacgcgct
     5761 caaccaggcc gcccatggtc tccaggccca gcgacagcgg aatcacgtcc agcagcagcc
     5821 agtcttcgcc cggcgcgcgg ttgcccgcca gaagattggc ctgctgcgcc gcgcccagcg
     5881 ccacgacttc atcgggattg aggttgacca ggggctcggt gccgaacaat tctcccaccg
     5941 cgcggcggat caccggcatg cgggtcgagc cgcccaccat gaccacgccc cgtacatcgg
     6001 ccgtcgacag cttggcgtcg cgcagcgcgc ggcgcgcgct gtccagcgtg cgctcgacca
     6061 gcggccgcgc ccaggactcg aagtcgccgc gcgtgatgtc gcgcgccacc tggcggccgt
     6121 cggccagcgt caaggcaaaa cggacggcct cctggtcgct cagggcctcg cgggccgtac
     6181 gagcggccac cagcaggccg cggcggtcgc ccggggtcag gtcgccgccc gcaggtaaat
     6241 tctccagcac atgctgcacg atgacggcgt cgaaatcatc gccgcccagc gcggtgtcgc
     6301 cgcccgtggc gatcacctcg aacacgccgc gcgacaggcg caggatggaa atgtcgaacg
     6361 tgccgccgcc caggtcgtac accgcgtaaa cgccctcgga cgcattgtcc aggccgtagg
     6421 cgatcgcggc cgcggtcggc tcgttgagca ggcgcagcac gttcaggccg gccaggcgcg
     6481 cggcatcgcg ggtagcctgg cgctgcgcat cgtcgaaata agccggcacg gtgatcaccg
     6541 cgcccaccag gtcgtcgccc agcacgtctt cggcgcgctg gcgcagcacg gccaggatct
     6601 gcgccgacac ttccaccggg ctcaggtcgc cctgcaccgt gcgcaggcgc accatgccgg
     6661 gcgcatcgac gaactcataa ggcgcgccgc tggcctgcgc ctcggccagc gagcggccca
     6721 tgaagcgctt gaccgacacg atggtgttgc gcgggtcggc cgcctgctgg gccagggccg
     6781 catggcccgt gggcgcccgt ccgtcgggca ggtactgcac cgccgacggc agcaagcgcc
     6841 ggccctcgtc gtcgggcagc acctcggcgg cgctgctgcg caccgcggca accagggaat
     6901 tggtcgtgcc caggtcgata ccgaccgcca gccggcgctg gtgcggcgcg ggcgactcgc
     6961 cgggctcgga aatctgcaat aaagccatgt tttttcttct gagtgtctag cgtgtatcca
     7021 tagcggcccg gagaaccggg cacgtcgcta tatcggccgc aatgcggcca gttcctgcgc
     7081 gagtctgtcg aggaacatcc attcgcgcac acggacggaa gcgccgatcg catcgccctg
     7141 gccatcgagc agcgcgccca cttgcctgcg catgccggct tgcgccgcct cgagctcggc
     7201 ctgcaagacc ggcacgacct gcgggtcgcg gcgggcatcg tcgagcatct cgcgccattc
     7261 catctgctgc atcagaaagg cggggtccat ggccgtgttg ctctcggtct gcaggtccac
     7321 gcccgcctgt tcgcacagat agcgcgcccg caagagcgga tcgcgcagct gccggtaggc
     7381 ctcgttggcg cgcgacgccc actgcatggc cacgcggcgc tcggccgcgc tggcggtggc
     7441 gaaccggtcg gggtgcacgc gtgccgccac gtcgcgccag gcatgttcca gcgcattctg
     7501 gtccagctcg aagcgcgccg gcagcccgaa caggctgaag tagtcgtccg ctgccaccgc
     7561 caccctacac cgtgaacgac tcgccgcagc cgcaggtcgc cttctcgttg gggttgcgga
     7621 acttgaagcc ttcgttcaag ccctcgcggg catagtcgag ctcggtgccg tcgatgtacg
     7681 gcaggctttt ggtatcgacg aacaccttga cgccgaagct ctcgaacact tcgtcggccg
     7741 tatcgggcgc gtcgacgtat tccagcttgt aggccatgcc cgaacaaccg gtcgtgcgca
     7801 cacccaggcg caagcccaca cccttgccgc gcttggccag gtagcgggtc acgtggttgg
     7861 ccgcctgttg agtcagggta atggccatgt caattcacag cagcggcttc agcctggacg
     7921 gcttcgccgt gcttggcgcg atagtcgctg acggcggcct tgatggcgtc ctcggccagg
     7981 atcgaacagt ggatcttgac cggcggcagg gccagttcct cggcgatctg cgtgttgcgg
     8041 atgctcatgg cctggtcgag cgtcttgccc ttgacccact cggtgaccag cgagctcgag
     8101 gcaatggccg aaccgcagcc gtaggtcttg aagcgcgcgt cttcgatgac gccgtcggcg
     8161 cccaccttga tctgcagctt catgacgtcg ccgcaggccg gcgcgccgac catgccggtg
     8221 ccgatcgatt cgtcgccctt gtcgaacgag cccacgttgc gggggttttc gtagtgatcc
     8281 agaactttgt tgctgtaaga cattttgact ctcctactga tttttcgtcc ccccgcccgt
     8341 ggggcggggg ttagggggtg ggctgtcacc ctcccccgca gggctcaata ttgacgcaac
     8401 gcgtcaatgt tgagcccact gcaccgaatt caggtcgatg ccttccttgg ccatttccca
     8461 gagcggcgac atgtcgcgca gcttgccgac gcggctcttg atgagctcga tg
//
LOCUS       DASWPO010000281        17697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1138289, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000281 DASWPO010000000
VERSION     DASWPO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 17697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17697
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138289"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1319
                     /locus_tag="VGC69_14845"
     CDS             51..1319
                     /locus_tag="VGC69_14845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0296604.1"
                     /translation="MRNNANLPESVDVAVIGGGIIGICTAYALAKAGVSTAVFEKGLL
                     ACEQSSRNWGWVRTLNRDMPEVPVSMRSNEIWDEIQAQAEVGFRRRGILYLQENADDA
                     ERHQRWLDQARDHYGFQGRLLGQAETRKRLPPSRRNWTGALYSDLDGVAEPQLATQGI
                     AGLVRRLGGQVFERCAVRGIEQSAGRVSAVVTEHGRVRASTVVLAGGAWSRLFCGNLG
                     VEFPQLKVRGSVLRTSPVEGGPESAINGKDFTCRKRADGGYTVSQFGASMADITPDSF
                     RLMGKFFKAWMANNTFVKVRFGKRFFEELAIPRTFGIEAITPYERNRVLDPTPSASGV
                     QQAWSRLVHAFPAFESARIAQSWAGYIDATPDAMPVMAQVRSVPGFYLASGFSGHGFG
                     FGPGAGEFMAGLIQGKPLHGDAHAFRLDRY"
     gene            1369..2388
                     /locus_tag="VGC69_14850"
     CDS             1369..2388
                     /locus_tag="VGC69_14850"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0296605.1"
                     /translation="MSNTASDLHRRAVIVDGLTFFSDGNTRDMLAGGVSAINLTITDM
                     PADYEQALKDAMAWRERGLDPQSGWLLVERAEDIPRAKAEGKLGLIMGWQNGKPLGDQ
                     PDRVKLFHTLGVRVIQLTYNEANLLGDGCLEPRNAGLSKLGIEMVAELNRTGIAIDLS
                     HCAEQTCLDAARHSTRPVFLTHANANAVIKRPRNKSDDVLRAVAATGGVIGCSIHAYL
                     AWRGDPKQQPLLRDFVANVKYIGELVGYEHVGIGTDFAAVDTYEAVRHVMVMSRTKYA
                     ASGGDVSSVFGDVMETRYPVETPNPSHFAKLTQALEEGGLSESQILGVLGGNFQRAFG
                     QAWAA"
     gene            2433..3401
                     /locus_tag="VGC69_14855"
     CDS             2433..3401
                     /locus_tag="VGC69_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296606.1"
                     /translation="MKFMRKARAVLAAAALLAGLPLAAKAAYPEHTIRIVVPYQAGGS
                     TDIVIRKFAELASTELGQSIIIDNRGGAGATLGARALQTSAPDGYTLAILPSPVFRMP
                     HIQDMGYDPTTDFTYVMMLSGYTLGVAVPADSPFKTWADLIAYARAHPGEIAYGTASV
                     GSASNVMMEEIASRNGVTWRHIPFKGESEVLQNILGGQLGVYAGSTTVLPLVQSGKMR
                     MLVTWGSHRSEQFPDTPTLQELDGTPPAYAPFGIAAPKGLPNEVLNTLQTRLGKVAKS
                     EAFKNVLTQYGQELVYLDSADYTKFALQSYKDEAEIVKKLGLANAK"
     gene            3449..4447
                     /locus_tag="VGC69_14860"
     CDS             3449..4447
                     /locus_tag="VGC69_14860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296607.1"
                     /translation="MPNATFDPSRRRLLGLASSIGAASFLPGLARADDWPSRPIRLIV
                     GYPPGGGTDTVARLTALHLSQTLGGQVIVDNRPGASGIVGTEMVAHASPDGYNILFAT
                     ASPLTGAPLTVKNLAYDPFTDLKPVIFIGGGPFILVANPSFPPNTLQELVAYARAHPG
                     EVNYASPGNSTADYFFCELLNIDAKIKTVAVAYKGSAALLNDVMGGYVQYTLDTPGTT
                     LPLIRAGKLKAIAVLSDKRLDIAPDIPTAVEAGYPRMVGGSWYGMLAPKGTPDAIVQK
                     LYQATKTAMASPEMVQGLAARDVIAQGLPPAEFGAFIKAEYNKWKEVTEKLGIQPQ"
     gene            4476..6218
                     /locus_tag="VGC69_14865"
     CDS             4476..6218
                     /locus_tag="VGC69_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY0296608.1"
                     /translation="MATQKKTDDLTLGSAPLSLRPVLAGLNHVISAGHYLATQAGMDV
                     LQAGGNAVDAGVAAGIALGVVQSDIVNFGGVAPILVYEAKSQKVSSISGLGYWPRAAS
                     LDLFLDRHGGTIPAGVLRTVIPAAPDAWITALERFGTLSFGDVAASAIRLAHEGYVMY
                     PLMAEVLADHRADYARWPSNAAVYLPGGRPPRAGEVFRQADLARTLQYMADEERAHAG
                     QGRAAGLAAARRAFYQGDIAAAIVKYHREEGGLVTAQDLRDFSVEVETPLTTRFRDVD
                     IYSCGYWCQGPSLLQMLNILESRDLKALGHNSAAYIHLLTEAIKLAFADREAYYGDPR
                     FSQVPHEMLLSKAYALQRLGLIDPAQAGPDVPQAGADAKTFTPDDTARTDPRLDTSYV
                     AVVDKHGNAFSATPSDGSYNSPVIPGTGIIASGRGSQSWAQAGHPYAVGAGRRPRLTP
                     NPSLAIKPGRYVMPFGTPGGDVQCQAMLQTFLNVEIFGMDLQQAVEAPRFASFGFPSS
                     FEPHSVQPGRLMIEDLVDRPVGETLAGFGHEVQWWRDRNWRAGAMCAVRHDLQTGIRW
                     GGADPRRPAYAAGC"
     gene            complement(6229..7551)
                     /locus_tag="VGC69_14870"
     CDS             complement(6229..7551)
                     /locus_tag="VGC69_14870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0296609.1"
                     /translation="MTFAPRELHLNVNALHSGFVPSAWRVPDADPHAFVDIDHYIRLA
                     RIAEAGKLDALFLADSAAIADQIRYRSITALEPTVLLAVLAAHTTHLGLIATASTSYN
                     EPYNIARRFATLDHVSHGRAGWNIVTTADLPSARNFGQKTVPDHGERYARAGEFTQIV
                     KDLWDSWDDAAFLGDKASGHFTDASRIRAIDHEGGHFSVQGPLNLPRTPQGRPVLVQA
                     GASGDGRDLAARHAEAVFSASQSFEESLVYRHDLNARAERHGRGRNAVKVLAGLTTII
                     GDTEQAAYRRRDELIDLIPLEYSINRLAGQLGVAPERLDPDQPLPQDLPLPNGGNGNH
                     TFFNVILATGRRTGHTVRELIRDMAGGGGHRVIVGTPEQIADDIEHWFRDGAADGFNL
                     MPDVLPGGLQDFVDGVVPILQKRGIFRAEYRGATLREHLGLARPEAGA"
     gene            complement(7595..8407)
                     /locus_tag="VGC69_14875"
     CDS             complement(7595..8407)
                     /locus_tag="VGC69_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0296610.1"
                     /translation="MHSPTRALRRLLPAIVLLACAFSAGAAELRVGFMPGPYRDAFAQ
                     GIAPQLEQKGYVIKYIEFSQGIQPNDAVERGNIDANIFQHSLFLEASNTQQGFHLAPI
                     VHVPTPPMGLYSKRYKSLDAVPDGATVTIPSDPVNAARALNMLADLGWIKVKPGVDPS
                     QVSERDVTDNPRKLRLVPLDTAQAPRSLEDADLAAIQGNFAVASGLPLTSALKLENMT
                     LPYVNVVAVREDKAHAQFADDIVAAYKSAQFQKVFNANPVWAGYRLPGYFAK"
     gene            8663..9415
                     /locus_tag="VGC69_14880"
     CDS             8663..9415
                     /locus_tag="VGC69_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HEY0296611.1"
                     /translation="MALSAAQAKPLKVGVRGGVDEQIWEVVAQVAKKQGLEVETVVIS
                     GAASPNEALNNGDLDANSFQHIPFFRDQVKQRGYKLAIVGNTLISPIAFYSKQYKSLE
                     SLPDGARIGLPSDPSNQTRALVILRDHGLITLRDGFDPYTGTATLEDVASNPHKLKFI
                     ETESVVLARSLPDVDAAAIINTFAYQVGLIATRDGIAVEKKEHNPYVNIIAVREQDRD
                     APWVPKLVAAYQSDEVRQFILSKYEGSVLPAF"
     gene            9460..10539
                     /locus_tag="VGC69_14885"
     CDS             9460..10539
                     /locus_tag="VGC69_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0296612.1"
                     /translation="MPGPTTRAQAAGGPPHIVFDGVGKTYPARGRVAVEALGEVSFSV
                     ARGRIFGIIGRSGAGKSTLLRSINMLERPSHGTVRVDGVDAGGLDEDGLVALRRKVGM
                     IFQHFNLMSAKTVAENVGLPLRVAGVDRATMARRVAELLALVGLQDKAQAYPAQLSGG
                     QKQRVGIARALVHDPEILLCDEATSALDPETTQSILALLRDINRELGLTIVLITHDMA
                     VIREICDEVLVLDQGRIVEQGEVWRLFGDPRAPATRALLRPLQHAVPAELAARLAADL
                     RGRPGKAVLSLRFTGEGGGLPLSALAALGEGAELLHGGLDRIGGHAQGALLVAVPAHV
                     YQQQSARIARLADHVEVIGYVAADA"
     gene            10520..11176
                     /locus_tag="VGC69_14890"
     CDS             10520..11176
                     /locus_tag="VGC69_14890"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEY0296613.1"
                     /translation="MSLPMLDRYVRAFLETLFMVGASGLISLSAGLLLAVVLTVTAPG
                     HLYASPRLNRALSVVVNVFRALPFIILLVALLPVTRYVVGTTLGTWAAVVPLSVSLTP
                     YFARIAQVSLNEIDPGLIEAARAMGCRRRHIIRHVLLPEALPGIVGGMTVSVIGMINA
                     SAMAGAVGAGGLGDMAIRYGYERYETQVMFEVILILIALVSLVQFAGERLARRVNHRK
                     "
     gene            11314..12405
                     /gene="mnmH"
                     /locus_tag="VGC69_14895"
     CDS             11314..12405
                     /gene="mnmH"
                     /locus_tag="VGC69_14895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY0296614.1"
                     /translation="MSHNKPVTVHDMRGFDALVDARSPAEFALDHVPGAINCPVLDDE
                     ERHLVGTLYVQQSAFEARRVGGAMVAANIAHHLREQLHDKPKNWRPLVYCWRGGMRSG
                     SFVTWLRLVGWDAQQLKGGYKAWRGHVIASNDGLAASLPLRVVCGPTGSAKTRVLHAL
                     ARLGEQVLDLEGLAGHRGSVLGAIPGVAQPSQKGFETGLYAAMSSFDLSRPVWVEAES
                     RKIGRLSLPEALVARLRSSPVVEIAASLSARLAYLLQDYAWLGDDPEDLCAKIASLKG
                     TQSNETLARWQAWARVRALPALFEDMMTLHYDPLYARSQGRNFQQLGRAVSVETRDLS
                     AAGIEDLARRITALPATADLSQPPRATGD"
     gene            complement(12362..12781)
                     /locus_tag="VGC69_14900"
     CDS             complement(12362..12781)
                     /locus_tag="VGC69_14900"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0296615.1"
                     /translation="MLKLEFDTEKDAANCEPFHCAAHRKPNGDNMVTQRKTDWARVHA
                     EAAADAPVAYDPQTDPYDPNDEAAVKEYWDEATLILKSPGQVVIRSPGRPPITIKRPT
                     LNMRVDADVLAHLRQSGKGWQTRVNRLLREAVEKGLL"
     gene            complement(12867..13010)
                     /locus_tag="VGC69_14905"
     CDS             complement(12867..13010)
                     /locus_tag="VGC69_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296616.1"
                     /translation="MLTLLRFFSRWLNKANQTHDQIYLDKCPDIATLEARMRLLEAER
                     SGW"
     gene            13095..14426
                     /locus_tag="VGC69_14910"
     CDS             13095..14426
                     /locus_tag="VGC69_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0296617.1"
                     /translation="MREPRYKAIVDDFTARIRSGRLAPGTQLPTTRELMRQHGVALAT
                     ASRAYAELGENGLVVGEAGRGTFVRDTSLPRGLGLEQRPARSGMIDMSVNYPVLPEQA
                     ELLRQGLRNLAASGDLDALLHCAPHGGRAHERQTVARHLRNRGIRVPGEQVLIVNGAQ
                     QGLAVSVMALLKPGDVLAVDALTYPGLKALAQDHHLDLASVPGRRQAEGMDLDKLEAL
                     CRKRPVKAIYVMPTLHNPLGWVMPEADRLRLAGLAEKHDFMIIEDGAYSFLAEPAPKP
                     VFTHAPQRTVFVSALSKSVASGLRLGFIAAPPPWMPALERAIRVSTWTTPSLSIALAC
                     QWIDSGVVDMLEDKKRKDARRRQALARRVLRDLDLTAHPASYFAWLALPEDVRADQVA
                     ARLLKQGIIVPTAEPFATTSAVPHALRVVVSGLPLDVLGQSLEAVRQAALG"
     gene            complement(14429..15124)
                     /locus_tag="VGC69_14915"
     CDS             complement(14429..15124)
                     /locus_tag="VGC69_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296618.1"
                     /translation="MLVLEDIRGGYDETPILNGISLTLEDRRIMTVAGTNGAGKSTLA
                     KAIVGLLPRVEGRILLDGNDLRALPVERRMGMGLAYVPQVANVFPSLTILVNLQVVVN
                     VPRQAQRIGEMFAMFPLLRQRASARAQTLSGGERQQLAFARSLMTRPTLMILDEPTAA
                     LSPSKVKESFERIARLPEAGVSVLIIEQRARESLAISDTGCIMDAGRVALAGKARELL
                     DNPRAAELYLGQA"
     gene            complement(15124..15882)
                     /locus_tag="VGC69_14920"
     CDS             complement(15124..15882)
                     /locus_tag="VGC69_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296619.1"
                     /translation="MMLDIQNLCKAFGGFEVLSGIDLSLPDGGLLGLIGPNGAGKSTL
                     FALISGLLPADAGRIRLFGADIDHAAPDARARAGMVRTFQVPREFRHLTVRQNLLAAA
                     PGQAGERLVNVFLQPRRVARQEAELAARADDTLSFLNLARVADAAAGSLSGGQKKLLE
                     LGRALMTEPRLILLDEPFAGVNPVLIEEIMERIQVLNQRGIGFIIIEHDLRALSKLTP
                     RLAVLDRGRIIAQGLPAEVLAMPVVRDAYFGGSA"
     gene            complement(15879..16766)
                     /locus_tag="VGC69_14925"
     CDS             complement(15879..16766)
                     /locus_tag="VGC69_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296620.1"
                     /translation="MTLYLLAIAVVTLINILLALGLTLQFGRAGLINFGHVGFYAIGA
                     YTSALLSQAGVPLPLCVLAAAALAALAAWPIGLVSLKLRDDYFAIVTLGFSETIRIII
                     TSETWLTHGVQGITAIPPLYAGLGTGYPSLLALTATLLLIVVVAAIAMHRISSSPFGR
                     VVEAIRDNEIAVKALGKNPARFKLGILAIGSALAGVAGAFYAHYIGYIVPDQFIPLVT
                     FYVWMAVIMGGSGRVSGAVVGSAILMFFLEGSRFLRDLLPGMATVEMASIRIGIIGLA
                     LILFTLYRPQGLMGDYTRK"
     gene            complement(16766..17626)
                     /locus_tag="VGC69_14930"
     CDS             complement(16766..17626)
                     /locus_tag="VGC69_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0296621.1"
                     /translation="MYSQLVLNGLIEGLVIGLGALAITLPFCISRFPNAATGDSMTLG
                     AYAGLTFAKWTGSLVLGGFLAAICGMLVSLLAYWLVFRRLAGRPVVALLIASIGIGFC
                     LRSVIGLVFGQGQYVFQIPLWRPWLLAGMRVNPLDAILAVVALGTMAAIFAILHLTPI
                     GRRMRALADDTDLARVSGIQPQVVMLWMWGLVGVTSAIAGIVLGIKTVVVPEAGWNML
                     VPLFAAAVLGGIGSPLGAVAAGLLLGVAQELCTPLVGFTYKISLAFVVLLLVLLVRPS
                     GLFGRPDQVR"
BASE COUNT         2890 a         5836 c         6133 g         2838 t
ORIGIN      
        1 gaatatcgat aatcataatg gatgatcagg ctatcgccaa gcggaaaccc atgaggaata
       61 acgcgaatct ccccgagtcc gtcgacgttg ccgtcatcgg cggcggcatc atcgggatct
      121 gcacggctta tgcgctggcc aaggccgggg tgagcacggc cgtgttcgaa aaaggcctgc
      181 tggcctgcga gcagtcctct cgcaattggg gctgggtgcg cacgctcaac cgcgacatgc
      241 ccgaagtgcc ggtctccatg cgctccaacg agatctggga cgagatccag gcgcaggccg
      301 aggtgggctt tcgccgccgt ggcatcctgt acctgcagga aaacgccgac gacgccgagc
      361 gccaccagcg ctggctggac caggcgcgcg accactacgg cttccagggc cggctgctgg
      421 gccaggccga gacgcgcaag cgcctgccgc cgtcccgccg caactggacg ggcgccctgt
      481 acagcgacct cgatggcgtg gccgagccgc aactggccac gcaaggcatc gccgggctgg
      541 tgcgccgatt gggcgggcag gtgttcgaac gatgcgccgt gcgcggcatc gaacagtcgg
      601 ccgggcgcgt gtcggccgtg gtcaccgaac acggccgggt gcgcgcttcg accgtggtgc
      661 tggccggcgg cgcctggtcg cggctgttct gcggcaacct gggcgtggag ttcccgcagc
      721 tgaaggtgcg cggctcggtg ctgcgcacct cgcccgtcga aggcgggccc gagtccgcca
      781 tcaacggcaa ggacttcacc tgccgcaagc gcgccgacgg cggctatacc gtgtcgcagt
      841 tcggcgcgtc gatggccgac atcacgcccg acagctttcg cctcatgggc aagttcttca
      901 aggcctggat ggccaacaac accttcgtca aggtgcgctt cggcaagcgc tttttcgagg
      961 agctggccat cccgcgcact ttcggcatcg aggccatcac gccgtatgaa cgcaaccgcg
     1021 tgctcgatcc cacgccctcg gccagcggcg tgcagcaggc ctggtcgcgg ctggtgcatg
     1081 cgtttccggc cttcgagtca gcgcggatcg cgcagtcgtg ggccggctac atcgacgcga
     1141 cgccggacgc catgcccgtg atggcgcagg tgcgcagcgt gcccggcttt tacctggcct
     1201 cgggtttttc ggggcatggc ttcggattcg gccccggcgc gggcgagttc atggccggcc
     1261 tgatccaggg caagccgctg cacggcgacg cgcacgcgtt ccggctggac cgatattgac
     1321 gcggccggcg gcggactatc cacctataca cagcaaggga gaatcattgt gagcaatacg
     1381 gcatccgatc tgcaccggcg ggctgtcatc gtcgacggcc tgactttctt cagcgacggc
     1441 aacacgcgcg acatgctggc cggcggggtc agcgccatca acctgaccat tacggacatg
     1501 cccgccgatt acgagcaggc gctgaaagac gccatggcct ggcgcgaacg cggcctcgat
     1561 ccgcaatcgg gctggctgct ggtcgagcgg gccgaagaca tcccgcgcgc caaggccgag
     1621 ggcaagctgg gcctgatcat ggggtggcag aacggcaagc cgctgggcga ccagccggat
     1681 cgcgtcaagt tgttccacac cctgggcgtg cgcgtcatcc agctcaccta caacgaggcc
     1741 aatctgctgg gcgacggctg cctggagccg cgcaacgcgg gcttgtccaa gctcggcatc
     1801 gagatggtgg ccgagctcaa ccgcaccggc atcgccatcg atctcagcca ttgcgccgag
     1861 cagacctgcc tggacgcggc gcgccacagc acgcggccgg tgttcctgac gcacgcgaac
     1921 gccaacgcgg tgatcaagcg cccgcgcaac aagtccgacg acgtgctgcg cgcggtcgcc
     1981 gcgacgggcg gcgtgatcgg ctgcagcatc catgcctacc tggcctggcg cggcgacccg
     2041 aagcagcagc cgctgctgcg ggatttcgtg gccaacgtga aatacatcgg cgagctggtg
     2101 ggctacgagc acgtgggcat cggcaccgac ttcgccgcgg tcgacaccta cgaggccgtg
     2161 cggcacgtca tggtgatgtc gcgcaccaag tacgcggcct cgggcggcga tgtctccagc
     2221 gtgttcggcg acgtcatgga aacccgctac ccggtggaaa cgcccaatcc ttcgcacttc
     2281 gcgaaactga cgcaggcgct ggaggagggc gggctgagcg agagccagat cctgggcgtg
     2341 ctgggcggca acttccagcg ggcgttcggc caggcctggg ccgcctgatt ccatcggcca
     2401 ttgcaacaac catccataac aaggggaaat tcatgaaatt catgcgcaag gcacgtgccg
     2461 tcctggctgc ggcggcattg ctggccggcc tgccgctggc cgccaaggcg gcttatcccg
     2521 agcacacgat acgcatcgtc gtgccgtatc aggcgggcgg ctccaccgat atcgtgatcc
     2581 gcaagttcgc cgaactggcg tccacggaac tgggccagtc catcatcatc gacaaccgcg
     2641 gcggcgcggg cgccacgctg ggcgcccgcg ccctgcaaac gtcggcgccg gacggctaca
     2701 ccctggccat cctgcccagc ccggtctttc gcatgcccca tatccaggac atgggctacg
     2761 acccgaccac ggacttcacc tacgtgatga tgctcagcgg ctatacgctg ggcgtggcgg
     2821 tgcccgccga ttcgccgttc aagacctggg cggacctgat cgcctatgcc cgcgcgcatc
     2881 cgggcgaaat cgcctacggc acggccagcg tcggcagcgc ctcgaacgtc atgatggagg
     2941 agattgcctc gcgcaacggc gtgacgtggc ggcacattcc cttcaagggc gagtccgagg
     3001 tgctgcagaa catcctgggc ggccaattgg gcgtgtacgc cgggtcgacc acggtgctgc
     3061 ccctggtgca gtcgggcaag atgcgcatgc tggtgacctg gggttcgcat cgcagcgaac
     3121 aattccccga tacgccgacc ctgcaggagc tcgacggcac gccgcccgcc tatgcgcctt
     3181 tcggcatcgc cgcgcccaag ggtttgccga acgaggtgct caacaccctg cagacccggc
     3241 tcggcaaggt cgccaagagc gaggctttca agaatgtcct gacgcaatac ggccaggagc
     3301 tggtctacct ggacagcgcc gactacacca agttcgcgct gcagtcctat aaggacgagg
     3361 ccgagatcgt caagaagctg ggcctggcca atgcgaaatg agccgccatt catgacgctg
     3421 caagggactc gcccggatga ttgcacctat gcctaacgcc actttcgatc cttcgcgccg
     3481 ccgcttgctg gggcttgcct ccagcatcgg cgccgcttcc ttcctgccgg gcctggcccg
     3541 ggcggacgac tggcccagcc ggcccatccg cctgatcgtg ggctacccgc ccggcggcgg
     3601 caccgatacc gtggcacggc tgacggcgct gcatctctcg caaacgctgg gcgggcaggt
     3661 catcgtcgac aaccggcccg gcgccagcgg catcgtcggc accgagatgg tcgcgcacgc
     3721 ctcgcccgac ggctacaaca tcctgttcgc gacggcttcg cccctgaccg gcgcgccgct
     3781 gacggtgaag aacctggcct acgacccctt caccgacctc aagcccgtca ttttcatcgg
     3841 cggcggcccc ttcatcctgg tggccaatcc cagcttcccg cccaacacgc tgcaggagct
     3901 ggtggcatac gcccgcgcgc atccgggcga ggtcaattac gcctcgccgg gcaacagcac
     3961 cgccgattat ttcttctgcg aactgctcaa catcgacgcg aagatcaaga cggtggccgt
     4021 ggcctacaag ggcagcgccg ctttgctcaa cgacgtgatg ggcggctatg tgcaatacac
     4081 gctcgacacc ccgggcacca cgctgccatt gatccgcgcg ggcaagctca aggccattgc
     4141 cgtgctgagc gacaagcgcc tggacatcgc gcccgacatc cccacggccg tcgaggcggg
     4201 ctatccccgg atggtgggcg gctcgtggta cggcatgctg gcgcccaagg gcacgcccga
     4261 cgccatcgtg cagaagctct accaggccac caagacggcc atggcctcgc ccgagatggt
     4321 ccagggcctg gccgcgcgcg acgtgatcgc gcagggcctg ccgcccgccg agttcggcgc
     4381 cttcatcaag gccgaataca acaagtggaa ggaagtcacc gagaaactcg gcatccaacc
     4441 ccaatgaagc gcgactgcgc atgaaggtgc ccgccatggc gacacagaaa aaaaccgacg
     4501 accttaccct gggcagcgcg ccgctgtcgc tgcggcccgt gctggccggc ctgaaccacg
     4561 tgatctcggc gggccattac ctggccacgc aagccggcat ggacgtgctg caggccggcg
     4621 gcaatgccgt ggacgccggc gtggccgccg gcatcgccct gggcgtggtg cagagcgaca
     4681 tcgtcaattt cggcggcgtc gcgcccatcc tggtgtacga agccaagtcg cagaaggtgt
     4741 cgagcatttc cggcctgggc tattggcccc gcgctgccag cctcgacctg ttcctcgaca
     4801 ggcacggcgg cacgattccc gccggcgtgc tgcgcacggt gatcccggcc gcgccggatg
     4861 cctggatcac ggcgctggaa cgtttcggca ccctgtcctt cggcgatgtg gccgcgagcg
     4921 ccatccgcct ggcgcatgaa ggctatgtga tgtatccgct gatggccgag gtgctggccg
     4981 accaccgggc cgactatgcc cgctggcctt cgaacgccgc ggtctacctg cctggcggcc
     5041 gtccgccgcg ggccggcgag gtgttccgcc aggccgacct ggcgcgcacc ctgcaataca
     5101 tggccgacga agagcgcgcc catgccggcc agggcagggc ggccgggctg gccgcggcgc
     5161 ggcgggcctt ctaccagggc gacatcgccg cggccatcgt caaataccac cgcgaagagg
     5221 gcggcctggt cacggcgcag gatctgcgcg atttttcggt cgaggtcgag acgccgctca
     5281 ccacgcgctt tcgcgacgtt gacatctaca gctgcggcta ctggtgccag gggccgtcgc
     5341 tcttgcagat gctcaatatc ctcgagtcgc gcgacctcaa ggcattgggg cacaactcgg
     5401 cggcctacat ccacctgctg accgaggcca tcaagctggc cttcgccgac cgcgaggcct
     5461 attacggcga tccgcgcttc agccaggtgc cgcacgagat gctgctgtcc aaggcttatg
     5521 ccctgcagcg cctgggcctg atcgatccgg cccaggccgg ccccgacgtg ccccaggccg
     5581 gcgccgacgc caagaccttc acgccggacg acacggcgcg caccgatccg cggctggaca
     5641 cgtcttatgt ggccgtggtg gacaagcacg gcaacgcctt ctcggccacg cccagcgacg
     5701 gttcctacaa cagtcccgtc attcccggca ccggcatcat cgcctcgggc cgcggcagcc
     5761 agtcgtgggc gcaagccggc catccctacg ccgtgggcgc gggacgccgg ccccggctca
     5821 cgcccaatcc ctcgctggcc atcaagcccg gccggtatgt gatgcccttc ggcacgccgg
     5881 gcggcgatgt gcagtgccag gccatgctgc agacctttct caacgtggag atcttcggca
     5941 tggacctgca gcaggcggtg gaagcgccgc gcttcgcgtc cttcggcttt ccgtcctcgt
     6001 tcgagccgca cagcgtgcag ccgggccgcc tgatgatcga agacctggtc gatcggccgg
     6061 tgggcgagac cctggccggg ttcggccacg aagtgcagtg gtggcgcgac cgcaactggc
     6121 gcgccggcgc catgtgcgcg gtgcggcacg acctgcagac gggcatacgc tggggcgggg
     6181 ccgacccgcg ccggccggcc tacgcggccg ggtgctgacg gcggctgttt aggcgccagc
     6241 ctcgggccgc gccaggccca gatgctcgcg tagcgtggcg ccgcggtatt cggcgcggaa
     6301 aatgccgcgc ttctgcagga tgggcacgac gccgtcgaca aaatcctgca ggccgcccgg
     6361 caacacgtcc ggcatcaggt tgaagccgtc ggcggcgccg tcgcggaacc agtgctcgat
     6421 gtcgtcggcg atctgctccg gcgtgccaac gatgacgcgg tgtccgccgc caccggccat
     6481 gtcgcgtatc agctcgcgca ccgtgtggcc ggtgcggcgg cccgtcgcca ggatgacgtt
     6541 gaagaaggtg tggttgccgt tgccgccgtt gggcaggggc aagtcttgcg gcagcggctg
     6601 gtccgggtcg agccgttcgg gcgcgacgcc cagctgtccg gccagccggt tgatgctgta
     6661 ttccagcggg atgaggtcga tcagctcgtc gcggcggcgg taggccgcct gctcggtatc
     6721 gccgatgatg gtcgtcagtc cggccagcac cttgacggcg ttgcggcccc ggccatggcg
     6781 ctcggcgcgg gcgttcaagt cgtgccggta gaccagcgac tcctcgaagg attgcgaggc
     6841 ggagaagacg gcttccgcat ggcgcgcggc caggtcgcgg ccatcgccgg aggcgccggc
     6901 ctggaccagc acgggccggc cttgcggcgt gcgcggcagg ttcagtggcc cctgcacgga
     6961 aaagtgcccg ccctcgtggt cgatcgcccg gatgcggctg gcatcggtga agtggccgga
     7021 ggccttgtcg cccaggaagg ccgcgtcgtc ccagctgtcc cacaaatcct tgacgatctg
     7081 cgtgaattcg ccggcgcgcg catagcgctc gccatggtcc ggcacggtct tctggccgaa
     7141 attgcgggcc gagggcaggt cggcggtggt gacgatattc catccggccc ggccatggct
     7201 gacgtggtcc agcgtggcga agcggcgggc gatgttgtag ggctcgttgt agctggtgga
     7261 ggcggtggcg atcaggccga ggtgggtggt gtgggccgcc agcacggcca gcagcaccgt
     7321 gggctccagg gccgtgatgg agcggtagcg gatctggtcg gcgatggcgg cgctgtcggc
     7381 caggaagagc gcgtccagct tgccggcctc ggcgatgcgc gcgagccgga tgtaatggtc
     7441 gatgtcgacg aaagcatgcg ggtcggcgtc gggcacgcgc caggccgaag gcacgaagcc
     7501 ggagtgcagg gcgttgacgt tcaggtgcag ttcgcgcggc gcgaaggtca tggaaggcat
     7561 ccggtctgac gcgggaggag tgcgcgcgtc ggcgttattt ggcgaaatag ccgggcaggc
     7621 ggtagcccgc ccagactgga ttggcgttga agaccttctg gaactgcgcg gacttgtagg
     7681 cggccacgat gtcgtcggcg aactgcgcgt gcgccttgtc ctcgcgcacg gccaccacgt
     7741 tgacataggg cagcgtcatg ttttccagct tcagggccga ggtgagcggc aggccggacg
     7801 ccacagcgaa attgccctgg atggcggcca ggtcggcatc ctccagcgag cggggggcct
     7861 gcgcggtgtc cagcgggacg aggcgcagct tgcgcgggtt gtcggtgacg tcgcgctcgg
     7921 acacctggga gggatcgacg ccgggcttga ccttgatcca acccaggtcc gcaagcatgt
     7981 tcagcgcgcg ggccgcattg accgggtcgg aggggatggt gaccgtcgcg ccgtcgggca
     8041 ccgcgtccag gctcttgtag cgcttggagt acaagcccat gggcggcgtg ggcacatgga
     8101 cgatgggcgc gaggtgaaag ccctgctgcg tgttgctggc ttccaggaac aaggaatgct
     8161 ggaagatatt ggcatcgata ttgcctcttt cgacggcgtc gttcggctgt atgccttggc
     8221 tgaattcgat gtacttgatc acatagccct tttgttcgag ctgcggcgcg atgccctgcg
     8281 cgaaggcgtc ccggtagggg ccgggcatga agcccacgcg cagttcggcc gcgccggccg
     8341 agaaggcgca ggccagcagg acgatcgcgg gcaacaggcg gcgcagggcg cgggtcgggc
     8401 tatgcatgac gggctccgga caggaaatga aagacaaaca gtctctgcca tgcctgccgg
     8461 cgaacctacg aaaaaatccc tgtttgctta tggcccggcc gggcatatcg acctaaccat
     8521 gaattcgttc cggtccgggc gatgcgttcc tagaatcgcc tgctctttct ctttctcttt
     8581 gtcgtaggcg aggcatttca tggtcagttc tctttttccg cgtcttttca agtccgcagc
     8641 gaccgcggcc gcgatctttg cgatggcgct gagcgcggcg caggccaagc ccttgaaggt
     8701 gggcgtgcgc ggcggcgtcg acgaacaaat ctgggaggta gtggcgcagg tcgccaagaa
     8761 acagggcctg gaggtcgaga ccgtggtgat cagcggcgcg gccagcccca acgaggcctt
     8821 gaacaatggc gatctggatg ccaattcgtt ccagcacatt ccgttcttcc gcgaccaggt
     8881 caagcagcgc ggctacaagc tggcgatcgt cggcaacacg ctgatctcgc cgatcgcgtt
     8941 ctattcgaag caatacaaat ccctggaaag cctacccgac ggcgcgcgca tcggcctgcc
     9001 cagcgacccg agcaaccaga cccgcgccct ggtgatcctg cgcgaccacg gcctgatcac
     9061 gctgcgcgac ggatttgatc cctataccgg cacggccacc ctggaagacg tcgcgtcgaa
     9121 tccgcacaag ctcaagttca tcgagaccga gtcggtcgtg ctcgcccggt cgctgccgga
     9181 cgtggatgcc gccgccatca tcaacacctt cgcctaccag gtcgggctga tcgccacgcg
     9241 cgacggcatc gcggtcgaga agaaggagca caacccctac gtcaacatca tcgcggtgcg
     9301 cgagcaggac cgcgacgcgc cctgggtgcc gaagctggtg gcggcctacc agtccgatga
     9361 ggtgcgccag ttcatcctga gcaagtacga ggggtcggtc ctcccggcgt tctgagcgtg
     9421 gacaacgtgt tcttcgacta cccgggaagg ccgggcaaaa tgccgggccc aaccacgcgg
     9481 gcgcaagcgg ccggcggtcc gccgcacatc gtgttcgacg gcgtgggcaa aacgtacccg
     9541 gcccgcggca gggtggcggt cgaggcgctg ggcgaggtgt cgttcagcgt ggcgcgcggc
     9601 cggatcttcg gcatcatcgg gcgcagcggc gcgggcaagt ccacgctgct gcggtcgatc
     9661 aacatgctgg agcggcccag ccatggcacg gtgcgcgtcg acggtgtgga tgccggcggc
     9721 ctggacgagg acggcctggt ggccttgcgc cgcaaggtgg gcatgatctt ccagcacttc
     9781 aacctgatgt cggccaagac cgtcgcggaa aacgtcggcc tgccgctgcg ggtggccggc
     9841 gtggaccgcg ccaccatggc gcggcgggtg gcggagctgc tggcgctcgt cggcctgcag
     9901 gacaaggcgc aggcctatcc ggcgcaattg tccggcgggc agaagcagcg cgtggggatt
     9961 gcccgggcgc tggtgcacga tccggaaatc ctcttgtgcg acgaggccac ctcggcgctg
    10021 gatccggaga cgacgcagtc catcctggcg ctcttgcgcg acatcaaccg cgaactcggg
    10081 ctgaccatcg tgctgatcac ccacgacatg gccgtcattc gcgagatctg cgacgaggtc
    10141 ctggtgctgg accaggggcg catcgtcgag cagggcgagg tatggcggct gttcggcgat
    10201 ccgcgggccc cggccacgcg cgcgctgctc aggcccttgc agcatgcggt gccggccgaa
    10261 ctggccgcca ggctggcggc cgacctgcgg ggccggccgg gcaaggccgt gctcagcctg
    10321 cgcttcacgg gcgaaggcgg cggcttgccg ctgtcggcgc tggccgcgct gggcgagggc
    10381 gccgaattgc tgcacggcgg cctggaccgg ataggcgggc acgcccaggg cgcgctgctg
    10441 gtggccgtgc cggcccatgt ctatcaacaa cagtcggccc gcatcgcgcg gcttgccgac
    10501 catgtcgagg taatcggcta tgtcgctgcc gatgcttgac cgctatgtgc gggccttcct
    10561 ggaaacgctg ttcatggtgg gcgcctcggg gctgatctcg ctgtccgcgg gactgctgct
    10621 ggccgtggtg ctgaccgtga cggcgcccgg ccatctttat gccagcccgc ggctgaacag
    10681 ggccttgtcc gtcgtggtga acgttttccg ggccttgccg ttcatcatcc tgctggtggc
    10741 cttgctgccg gtgacgcgct atgtcgtcgg caccacgctg ggcacctggg ccgcggtggt
    10801 gccgctgtcg gtcagcctca cgccgtactt cgcgcgcatc gcgcaggtca gcctgaatga
    10861 aatcgatccg ggcctgatcg aggcggcgcg ggccatgggc tgccggcggc ggcacatcat
    10921 ccgccacgtg ctgctgcccg aggccctgcc tggcatcgtc ggcggcatga cggtgagcgt
    10981 gatcggcatg atcaacgcct cggccatggc cggcgcggtg ggcgcgggcg ggctgggcga
    11041 catggcgatc cgctatggct acgaacgcta cgagacccag gtcatgttcg aggtcatcct
    11101 catcctgatc gccctggtgt cgctggtgca gttcgcgggg gagaggctgg cgaggcgcgt
    11161 gaaccatcgc aagtagcccg ggctgggcgg gtcttataat cctggcattc ctgccaggtg
    11221 ggcggttcgt ccccgcaggg ggaaatgggt ttgaaacccc ggggtttgaa gccctgtcca
    11281 aatgtcgcaa cccgaaccgc ccgcaggtac ttcttgagtc acaacaaacc cgtcaccgtg
    11341 cacgatatgc ggggtttcga cgccctggtc gatgcgcgct cgcccgccga attcgcgctc
    11401 gaccacgtgc cgggcgccat caactgcccg gtgctcgacg acgaggagcg ccacctcgtc
    11461 ggcaccctct acgtgcagca atcggccttc gaggccaggc gcgtgggcgg cgccatggtg
    11521 gccgccaata tcgcccacca cctgcgcgag cagctccacg acaagccgaa aaactggcgc
    11581 ccgctggtgt attgctggcg cggcgggatg cgcagcggca gcttcgtgac ctggctgcgc
    11641 ctggtgggct gggacgccca gcagctcaag ggcggctaca aggcctggcg cggccacgtg
    11701 atcgccagca acgacggcct ggccgcgtcg ctgccgctgc gggtggtgtg cggccccacg
    11761 ggcagcgcca agacgcgcgt gctgcacgca ctggcccgcc tgggcgagca ggtgctggac
    11821 ctggagggcc tggccgggca tcgcggctcg gtgctgggcg ccattccggg cgtggcgcag
    11881 ccttcgcaaa aaggtttcga gaccggcctc tatgcggcca tgtcgtcctt cgacctgtcg
    11941 cgtccggtgt gggtcgaggc cgagagccgc aagatcggcc ggctgagcct gcccgaagcc
    12001 ctggtggccc gcctgcgttc gagccccgtg gtcgagatcg ccgcgtccct gagcgctcgc
    12061 ctggcttacc tgctgcagga ctatgcctgg ctgggcgacg atcccgagga tctttgcgcc
    12121 aagatcgcga gcctgaaggg cacccaatcc aacgagacgc tggcgcgctg gcaggcctgg
    12181 gcgcgcgtgc gcgcgctgcc ggccctgttc gaagacatga tgaccctgca ctacgacccg
    12241 ctttacgcac gctcgcaggg ccgcaatttc cagcagctgg ggcgggcggt aagcgtcgaa
    12301 acgcgggatc tctcggcggc tggcatcgag gatctggcgc gccgtattac ggcattgccc
    12361 gctacagcag acctttctca accgcctcgc gcaacaggcg attgacgcgg gtttgccagc
    12421 ctttgcctga ttggcgcaag tgtgccagca cgtcggcgtc cactctcatg ttcagggtag
    12481 ggcgcttgat ggtaataggc ggtcggccag gggatcgaat gacgacttgc ccaggcgact
    12541 taaggattag cgtggcctcg tcccagtatt ctttgacggc cgcttcgtca ttgggatcgt
    12601 aggggtctgt ctgcgggtcg taggcgacgg gggcatctgc cgcagcctcg gcgtggacgc
    12661 gggcccagtc agttttccgt tgggtaacca tattgtctcc gttcggtttt cgatgcgcgg
    12721 cgcagtgaaa tggctcgcaa ttcgcggcgt ctttctcggt gtcgaattcc agtttcagca
    12781 caataattgt attggcaaat attattggat gccaagagaa attaccgtgc ctgaacctgt
    12841 atatgcctac aggagagact agtccgtcac cacccggagc gctctgcctc caatagccgc
    12901 atgcgcgcct caagcgtggc gatgtcggga catttgtcca gatagatctg gtcgtgcgtt
    12961 tggttggcct tgttcagcca tcgtgaaaaa aatcggagca gcgtcaacat caatgtctct
    13021 ttgaatcggg tttttcatag cgtatgcttg gtatccagta caaacaaaaa attgttctag
    13081 atacaagtcc agccatgcgc gaaccccgct acaaagccat cgtcgacgac ttcacggcac
    13141 gcatccgctc gggcaggctc gcgcccggca cgcagctgcc caccacgcgg gagctgatgc
    13201 ggcagcatgg cgttgccctg gcaaccgcct cgcgcgccta tgccgaactg ggcgagaacg
    13261 gcctggtggt gggcgaggcg gggcgaggca ctttcgtgcg cgacacctcg ctgccgcgcg
    13321 gcctgggcct ggagcaacgc ccggcgcgct cgggcatgat cgacatgagc gtgaactacc
    13381 cggtgctgcc ggagcaggcc gagctattgc ggcagggcct gcgcaacctg gcggcctcgg
    13441 gcgatctcga cgccttgctg cattgcgcgc cgcacggcgg gcgggcgcat gaacgccaga
    13501 cggtggcgcg gcacctgcgc aatcgcggca tccgcgtgcc gggcgagcag gtgctgatcg
    13561 tcaacggggc ccagcagggc ctggcggtgt cggtgatggc cctgctcaag ccgggcgacg
    13621 tgctggccgt cgacgcgctg acataccccg ggctgaaagc cctggcgcag gaccaccacc
    13681 tggatcttgc ttccgtcccg ggacgcaggc aggctgaagg catggacctg gacaagctcg
    13741 aggcgctttg ccgcaagcgc ccggtgaagg ccatctatgt catgcccacg ctgcacaacc
    13801 cgctgggctg ggtcatgccg gaggccgacc ggctgcgcct ggccggcctg gccgagaagc
    13861 atgatttcat gatcatcgaa gacggggcct attcgttttt ggccgaaccc gcgcccaagc
    13921 cggtcttcac ccacgcgccg cagcgcacgg tgttcgtctc ggccctgtcg aaaagcgtcg
    13981 cgtcgggcct gcgcctggga ttcatcgcgg cgccgccgcc gtggatgccg gcgctggagc
    14041 gcgccatccg cgtctcgacc tggacgacgc cgtcgctgag catcgccctg gcctgccagt
    14101 ggatagacag cggcgtggtc gacatgctgg aagacaagaa gcgcaaggac gcccgccgcc
    14161 gccaggccct ggcgcgccgc gtgctgcgcg atctcgatct gaccgcgcac ccggcgtctt
    14221 atttcgcgtg gctggccctg ccggaagacg tgcgggccga ccaggtggcg gcgaggttgc
    14281 tgaagcaggg catcatcgtg cccacggccg agcctttcgc cacgaccagc gccgtgccgc
    14341 atgcgttgcg ggtggtggtg agcggcctgc cgctcgacgt gctggggcaa tcgttggagg
    14401 cggtgcggca ggcggcgctg gggtagcgtc aggcctggcc caggtagagt tcggcggccc
    14461 gggggttgtc gagcagttcc cgggccttgc ccgccagggc cactcggccc gcatccatga
    14521 tgcagcccgt gtcggagatc gccagcgact ccctcgcgcg ctgctcgatg atcagcaccg
    14581 agacgccggc ctcgggcagc cgggcgatcc gctcgaacga ttccttgacc ttgctgggcg
    14641 acagcgcggc cgtcggctcg tcgaggatca tcagcgtcgg ccgggtcatc agcgaccgcg
    14701 cgaaggcgag ctgctggcgc tcgccgcccg acagcgtctg ggcgcgcgcc gaggcccgct
    14761 gcctgagcag gggaaacatg gcgaacatct cgccgatgcg ctgcgcctgg cggggcacgt
    14821 tgaccacgac ctgcagattc accaggatgg tcagcgaggg aaagacgttg gccacctgcg
    14881 gcacgtaggc caggcccatg cccatgcgcc gttccaccgg cagggcgcgc aggtcgttgc
    14941 cgtcgagcag gatcctgccc tcgacgcgcg gcagcaggcc gacgatggcc ttggccaggg
    15001 tggacttgcc ggcgccgttc gtgccggcca cggtcatgat gcggcggtct tcgagcgtca
    15061 gggaaatgcc gttgaggatc ggggtttcgt cgtaaccgcc gcggatgtct tccagcacca
    15121 gcatcaggcc gagcctccga aataggcgtc gcgcacgacg ggcatggcca gtacttcggc
    15181 gggcagcccc tgtgcgatga tccggccccg gtcgagcacg gccaaccggg gcgtgagctt
    15241 ggacagcgcc cgcaagtcgt gctcgatgat gatgaagccg atgccgcgct ggttgagcac
    15301 ctggatgcgt tccatgattt cttcgatcag caccgggttg acgcccgcga aaggctcgtc
    15361 gagcaggatc aggcgtggct cggtcatcag cgcccggccc agttcgagca gtttcttctg
    15421 gccgcccgac aggctgccgg cggccgcgtc cgcgacgcgc gcgagattga ggaaggacag
    15481 ggtgtcgtcc gcgcgggcgg ccagttcggc ctcttgccgg gcgacgcgcc gcggctgcag
    15541 gaagacgttg acgagccgct cgccggcctg gccgggcgcg gcggccagca ggttctggcg
    15601 caccgtgaga tggcggaact cgcgcggcac ctgaaaggtc cgcaccatgc ccgccctggc
    15661 gcgggcgtcg ggcgccgcgt ggtcgatgtc cgcgccgaac aggcggatgc ggcccgcgtc
    15721 ggccggcagt aagcccgaga tgagcgcgaa cagcgtcgac ttgccggcgc cgttcgggcc
    15781 gatcagcccg agcaggccgc cgtcgggcag ggacaggtcg atgccgctca ggacttcgaa
    15841 gccgccgaag gctttgcaga ggttctggat gtcgagcatc atttgcgcgt gtagtcgccc
    15901 atgagtccct ggggacggta tagcgtgaag agaatgagcg ccaggccgat gatgccgatg
    15961 cgtatcgagg ccatttccac ggtggccatg cccggcagca ggtcgcgcag gaagcgcgag
    16021 ccttccagga agaacatcag gatcgccgag cccacgacag cgcccgacac gcggccggag
    16081 ccgcccatga tgacggccat ccatacatag aaggtcacca gcgggatgaa ctgatcgggc
    16141 acgatgtagc cgatgtagtg cgcgtagaag gcgcccgcca ccccggccag cgccgagccg
    16201 atcgccagga tgcccagctt gaaccgggcc gggttcttgc ccagggcctt gaccgcgatt
    16261 tcattgtcgc ggatggcctc gacgacgcgc ccgaacggcg aggacgagat gcggtgcatg
    16321 gcgatagcgg cgacgacgac gatcagcagc agcgtggcgg tcagggccag cagggaaggg
    16381 tagcccgtgc ccaggccggc gtagagcggc gggatggccg tgatgccctg caccccgtgc
    16441 gtgagccagg tttcgctcgt gatgatgatc cgtatcgtct cggagaaccc caaggtcacg
    16501 atggcgaagt agtcgtcgcg cagcttcagc gagacgagcc cgatgggcca ggcggccagc
    16561 gcggccagcg ccgcggcggc caggacgcac aggggcagcg gcacgccggc ctgcgacagc
    16621 agggcgctgg tgtaggcgcc gatggcgtag aagcccacgt gcccgaaatt gatcagcccg
    16681 gcgcggccga actgcagcgt caggcccagg gcgagcagga tgttgatgag tgtgacgacg
    16741 gcgatggcaa gcagatagag cgtcatcagc gcacctgatc gggacggccg aacagcccgc
    16801 tggggcgcac cagcagcacc agcagcagca cgacgaaggc cagggatatt ttgtaggtga
    16861 agcccaccag cggcgtgcac agttcctgcg cgacgcccag cagcagcccg gctgcgaccg
    16921 cgcccagcgg cgaaccgatg ccgcccagca ccgcggcggc gaacaggggc accagcatgt
    16981 tccagccggc ctccggcacg acgaccgtct tgatgccgag cacgatgccg gcgatggcgc
    17041 tggtgacgcc gaccaggccc cacatccaga gcatgaccac ttgcggctgg atgcccgaca
    17101 cgcgggcgag gtcggtgtcg tcggccagcg cgcgcatgcg gcgtccgatg ggcgtcaggt
    17161 gcaggatggc gaagatcgcc gccatggtgc ccagcgccac caccgccagg atcgcgtcga
    17221 gcgggttgac gcgcatgccg gcgagcagcc agggacgcca cagcgggatc tggaagacat
    17281 actggccctg gccgaagacc aggccgatga cggaccgcag gcagaagcca atgccgatgg
    17341 aggcgatcag cagcgcgacc acgggccggc cggccaggcg ccggaacacc agccaatagg
    17401 ccagcaggct gaccagcatg ccgcagatcg cggccaggaa cccgccgagc accagcgatc
    17461 ccgtccattt cgcgaacgtg agcccggcat aggcgcccag ggtcatgctg tcgcccgtgg
    17521 cggcattggg gaatcgcgag atgcagaagg gcagggtgat ggccagggcg cccagcccga
    17581 tgaccaggcc ttcgatcagg ccgttgagca ccagttggct atacatcgtt tccttggggt
    17641 atgcggcagc attgccgggc ctggcggccc ggtttctttt cgttgggtgg cgtgttc
//
LOCUS       DASWPO010000282         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2657570, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000282 DASWPO010000000
VERSION     DASWPO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657570"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..172
                     /locus_tag="VGC69_14935"
     CDS             20..172
                     /locus_tag="VGC69_14935"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY0296622.1"
                     /translation="MEQHAAFYRQINLEEACREHLQIIAALERGDRDQAAALMRRHLL
                     TSSDLS"
     gene            370..1647
                     /locus_tag="VGC69_14940"
     CDS             370..1647
                     /locus_tag="VGC69_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0296623.1"
                     /translation="MPFTSNRRFKREPQLFVEADGMYYIRPDGSRVLDGMAGLWCVNA
                     GHRRPEIAEAIAKSAYRLDYAPSFQMSHPQAFAFAEALVAEAPEGFSNVFFSNSGSEA
                     VDSALKIALAYHRARGDAQRVRLIGRERAYHGVGFGGLSVSGIAGHRKPFGNLLPWVD
                     HLPHTYDRAAMGYSRGQPAWGAHLADELERIVALHDASTIAAVIVEPVSGSTGVLVPP
                     VGYLQRLREICDKHGILLIFDEVISGFGRVGGAFAADFFGVAPDIITSAKGLTNGAVP
                     MGATLVRRHVYDAFMQGPAGAIELPHGYTYSGHPLACAAGLAALDIYRREGLFERAGA
                     LSDRWADAAHSLKGLPHVVDIRNIGLLAAVELSPVAGQPGQRGYAAHLGCLARDCLIR
                     AAGDTMLLSPPLVIGEAQIDQLFTTLGDVLRTL"
     gene            complement(1672..2238)
                     /locus_tag="VGC69_14945"
     CDS             complement(1672..2238)
                     /locus_tag="VGC69_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0296624.1"
                     /translation="MWNLISNAGDAALTLPLAALCAAWLACAQRRLAWHWLLALAIGM
                     ALVGATKILHAGCGTEVAPLGFRVISGHTTLATAIWPTLLGLFSYALARRPAPGAVLG
                     LAIGALIGVARVFDHSHTVSEVIAGWLLGCIVAVPSLRALARGGLRLPHAWAAALIAA
                     LILVAFYGHHAPFEVMINRYAAGLCARV"
     gene            complement(2248..>3739)
                     /locus_tag="VGC69_14950"
     CDS             complement(2248..>3739)
                     /locus_tag="VGC69_14950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY0296625.1"
                     /translation="PLPVPRRRIQMRRIAPRARAISHIVLVCLTLAGLDGWHSWHTRE
                     AALRDMRSDAASLARNAAQHADDVFTLVNVVLQGVQQSIAIHGIKPDAMPALQQQLQM
                     RANTLSQIKVLNVYDPQGELLAYSVDNPLSMASVSDREYMQFHRAHIDPGMHIGPPIV
                     SRSTGALIIPVSMRFSDAYGRLKGVILAGVSIDNFRAYYDSLQLGGHGTISMYAPDGT
                     LAVKHPFRQEEVGITRAKELANGIERHFSYQHLAHYPITLVAGLSQSDALQGWWRDTL
                     LHAAVMLIIIAVISLQGWRMIRQDQAIKHTLRRQALVDELTGLANRRQFDQSLAREMA
                     GASRPTLPLGLILFDVDYFKQYNDVYGHIAGDNCLREISRLIRGAIKRPGDLVARYGG
                     EEIAVLLPGTDAAGALSMAEGIRQVISDARIPHAGSPFGIITLSAGAHAELPSARHDR
                     ADTLLMAVDTALYTAKRQGRNQSCRAEESESPPPSRPYGGESTIQA"
BASE COUNT          560 a         1208 c         1356 g          615 t
ORIGIN      
        1 gcagacccag ctgcgccggc tggaacagca cgcggctttc tatcgccaga tcaacctgga
       61 ggaagcctgt cgggagcatc tgcagatcat cgcggcgctg gagcggggcg accgcgacca
      121 ggcggcggcg ctgatgcggc ggcatttgct gacttcgagc gatttgagct gaatgcggct
      181 tcgccgtgtc cggcgggctc gatgagggcc ctatattgca cttaatgttc aattagtaca
      241 aaatagatcg aggtcatccc gaccgtccga tccggagcca tcatgtcctc tcccgtcgcc
      301 gggcctaccg gccagccgcc gcaacagaac gctcttcccg ccgccgaccc cgccctcgac
      361 gcctactgga tgccgtttac ctccaaccgg cgtttcaagc gcgagccgca gttgttcgtc
      421 gaggccgacg gcatgtacta catccgcccg gatggcagcc gcgtgctcga cggcatggcc
      481 ggcctgtggt gcgtcaacgc cggccaccgc cgccccgaaa tcgccgaggc catcgccaaa
      541 agcgcctacc ggctcgatta cgcgccgtcg ttccagatga gccacccgca ggccttcgcg
      601 tttgccgagg cgctcgtggc cgaggcgccg gaaggctttt ccaatgtgtt cttttccaat
      661 tccggatcgg aagccgtgga ttcggcgctg aagatcgcgc tggcctatca tcgcgcccgc
      721 ggcgacgcgc agcgcgtgcg cttgatcggg cgggaacgcg cctaccacgg cgtcggcttc
      781 gggggactct cggtgtcagg catcgccggt caccgcaagc cgttcggcaa tttgctgccg
      841 tgggtcgatc acttgccgca tacctacgac cgggcggcca tgggctactc gcgcggccag
      901 ccggcttggg gcgcgcacct ggccgatgaa ctggagcgca tcgtcgcgct gcacgatgcg
      961 tcgacgatcg cggcggtgat cgtggagccg gtgtccggct ccaccggcgt gctggtgccg
     1021 cccgtgggtt atctgcagcg cctgcgcgag atctgcgaca agcacggcat cctgctgatc
     1081 ttcgacgagg tgatctcggg cttcggccgt gtcggcggcg ccttcgccgc ggatttcttt
     1141 ggcgtcgcgc ccgacatcat caccagcgcc aagggcctga ccaatggcgc cgtgccgatg
     1201 ggcgccacgc tggtgcgcag gcacgtgtac gacgcattca tgcaaggccc tgccggcgcc
     1261 atcgagctgc cgcacggcta cacctactcg ggtcatccgt tggcgtgcgc ggccggcctg
     1321 gcggcgctgg acatctaccg ccgcgagggg ctgttcgagc gggccggcgc cctgtcggat
     1381 cgctgggccg acgcggcgca cagcctcaag gggctgccgc acgtggtcga tatccgcaac
     1441 atcggcctgt tggccgcggt ggaattgtcg ccggtggcgg gccagccggg gcagcgcggc
     1501 tatgccgccc atctgggctg cctggcgcga gactgcctga tccgcgccgc cggcgacacc
     1561 atgctgctgt cgccgccgct ggtgatcggc gaagcgcaga tcgaccagct cttcaccacc
     1621 ttgggcgacg tgctcaggac gctgtgaggc cggcgtcccc gcggcgcttg atcacacgcg
     1681 cgcgcacagc cccgcggcat agcggttgat catgacctcg aacggggcgt ggtggccgta
     1741 gaacgccacg aggatgagtg ccgcgatgag cgcggccgcc caggcgtgcg gcaggcgcag
     1801 gccgccgcgc gccagggccc gcagggacgg gacggcgacg atgcagccca gcagccatcc
     1861 cgcgatgacc tccgagacgg tgtgcgagtg atcgaacacc cgggcaacgc cgatgagggc
     1921 gccgatggcc agccccagga cggcgccggg cgccggccgg cgcgccaggg catagctgaa
     1981 caggcccagc agggtgggcc agatggccgt ggccagggtg gtgtgcccgc tgatcacgcg
     2041 aaagccgagc ggcgccactt cggtgccaca gccggcgtgc aggatcttgg tcgcgccgac
     2101 cagggccatg ccgatcgcca gggccaggag ccaatgccag gcaaggcgcc gttgcgcgca
     2161 ggccagccag gcggcgcaaa gcgcggccag gggcagtgtc agggcggcgt cgccggcgtt
     2221 gctgatgaga ttccacatgg ttttgggtca ggcctggatc gtcgattcgc cgccgtaggg
     2281 gcgggaaggc ggcggcgatt cgctctcttc ggcccggcac gattggttgc ggccctgccg
     2341 cttggccgta tagagcgccg tgtcgacggc catgagcagg gtgtcggcgc ggtcgtgccg
     2401 ggccgaaggc agctcggcgt gggcgccggc gctgagcgtg atgatgccga agggactgcc
     2461 ggcatggggg atgcgcgcgt cgctgatcac ctggcggatg ccctccgcca tggacagggc
     2521 gcccgccgcg tcggtgccgg gcaggagcac ggcgatttct tcgccgccgt agcgggcgac
     2581 caggtcgccc ggccgcttga tggcgccgcg gatgaggcgg ctgatctcgc gcaggcagtt
     2641 gtcgccggcg atatggccat agacgtcgtt gtattgcttg aagtagtcga catcgaacag
     2701 gatcaggccc agcggcagcg tgggacggct cgcgcccgcc atttcccggg ccagggattg
     2761 gtcgaactgg cgccggttgg ccaggcccgt gagttcatcg accagcgcct gccggcgcag
     2821 ggtgtgcttg atcgcctggt cttgccggat catacgccag ccctgcaggc tgatgacggc
     2881 gatgatgatc agcatcacgg ccgcgtgcag cagggtgtcg cgccaccagc cctgcagggc
     2941 gtcgctctgg gatagcccgg cgacgagggt gatggggtag tgggccaggt gctggtagct
     3001 gaagtggcgt tcgatgccgt tcgcgagctc tttggcccgg gtgatgccca cctcttcctg
     3061 cctgaacgga tgcttcacgg ccagggtgcc gtcgggcgca tacatggaga tggtgccgtg
     3121 cccgcctagc tgcaggctgt cgtagtaggc gcggaagttg tcgatcgaca cgccggccag
     3181 tatcacgccc ttgaggcgtc cgtaggcgtc ggagaaccgc atggagaccg ggatgatcag
     3241 ggcgccggtg gagcggctga cgatgggcgg gccgatgtgc atgccgggat cgatgtgggc
     3301 gcgatggaat tgcatgtatt cacggtccga gacgctggcc atggacaagg ggttgtctac
     3361 cgaataggcc agcagctcgc cctggggatc gtagacgttc aggaccttga tctgcgacag
     3421 cgtgttggcc cgcatctgca gctgctgctg cagcgcgggc atggcatcgg gcttgatgcc
     3481 atggatggca atggactgct gcacgccctg cagcacgaca ttgaccaggg tgaagacgtc
     3541 gtcggcgtgc tgggccgcgt tgcgggccag gctggccgcg tcgctgcgca tgtcccgcaa
     3601 ggcggcctcg cgcgtgtgcc agctgtgcca tccgtcgaga ccggccagcg tcaggcacac
     3661 cagcacgatg tggctgatgg ctcgggcgcg cggggcgatg cgtctcatct ggatgcgacg
     3721 acgagggacg ggtaggggc
//
LOCUS       DASWPO010000283         4913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_154240, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000283 DASWPO010000000
VERSION     DASWPO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154240"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VGC69_14955"
     CDS             <1..358
                     /locus_tag="VGC69_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0296626.1"
                     /translation="FMVAYDGSPTADKTVDIVSRSPLLAGLPAHLVMVGRKSAMAQAG
                     LERARTQLARAGFAAQATHIPGEPTAALAAYAEQHGIDMLAMGAYGRTRLRHLVLGST
                     TTAILGQLRLPLLILR"
     gene            complement(341..1636)
                     /locus_tag="VGC69_14960"
     CDS             complement(341..1636)
                     /locus_tag="VGC69_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY0296627.1"
                     /translation="MTSHTCSSFLSLALGLLSVTAYAQDATSADAGTFHSCLASLKPD
                     ATQHGVSASTYDTQVALLSPDMTVLPLLDAQPEFKQPIWDYLAGLVDQERVDDGLAAM
                     AKWRAEFDRAQAQFGVDAAVVAAVWGVESNFGRIQGGRPLLASLSTLSCFGRRQSYFR
                     GEFYATLKILQDERIPADKLMGSWAGAFGQTQFMPSTYQRLAVDFDGDGRRDLIDSVP
                     DALGSTANFLKQAGWQPGLPWGFEVILPAGLDTSNAGRRNKQSLSSWVARGVKRADGQ
                     PMPAGDPMAGLLLPAGPQGPAFLVTRNFDAIYAYNAAESYALAIAHLSDRLKGGGPFV
                     TPWPTDDPGLSRAERREVQALLLGQGYDVGQPDGIIGARTRQALQTVQQQLGLPADGR
                     AGMKTLQALRAMPLKPSAAPPAAAPPQAATPTQPSTQNQ"
     gene            complement(1754..2545)
                     /locus_tag="VGC69_14965"
     CDS             complement(1754..2545)
                     /locus_tag="VGC69_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0296628.1"
                     /translation="MRSIISAASLREQALRALQVKDSPAKLAAVASLDDTAPVDPLAP
                     LDEPADLPGRPARPELVQPDQLAPRPVHTPQGRAAMLHALAHIEFNAINLALDAIWRF
                     AGQPEAYYRDWLRVAREEAHHFDLLNRHLATLGHAYGDFPAHNGLWEMAEKTRGDLLA
                     RLALVPRTLEARGLDASPPIRRKLAGAGDMQAAAILDVILRDEIGHVAIGNHWYRHAC
                     EQRGLDPIVAYAKLAQDYGAPKLRGPFNLEARRAAGFTDAELEAL"
     gene            2631..3764
                     /locus_tag="VGC69_14970"
     CDS             2631..3764
                     /locus_tag="VGC69_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0296629.1"
                     /translation="MPPSDHFSLETSLEAFDRRQWNALAGDQPFVRHEFLSALHETGC
                     AAPDTGWTPYFLALRRAGALAGAVPLYLKTHSRGEYVFDYAWADAYERHGLRYYPKLL
                     SSVPFTPVTGARLLAASHADRAALALGLIELAQQLKLSSLHLLFPRDEDLAALREAGY
                     MVREGVQFHWLNPGYAGMDDFLAAMSHDKRKKIKQDRKKVAQAGIRYRWLRGAQIDAA
                     SLDFFYACYCRTYYEHGNPPYLTAEFFQRVHEQMPDAMVLILAMQGETPVAAALNLQD
                     GEALYGRYWGSTVFVSGLHFETCYMQAIEYCIANRVARFEGGAQGEHKVARGLLPTPT
                     WSAHWIADPRFAEAIEDFLNRETAAMQDYLGELEMHAPFKRAS"
     gene            3761..>4913
                     /locus_tag="VGC69_14975"
     CDS             3761..>4913
                     /locus_tag="VGC69_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0296630.1"
                     /translation="MTRQSHAHLTTRLPWHQYLLLALCIVIPLCAGVLWTWHGARRNQ
                     EKTAADAAQNIEHQLGIVLNRVRQALLQLTPLTTLPCERLEPELTLRTATIPRVRTLT
                     VMDGHRIYCSSNGPLGTYNLEDMKGAPGDLPPSDWSAILGGTPVVPDRPVLVVGHPVP
                     ASSKTVLAYVDDRNLRDILRAGLPPDTFLQTDLNIGGGLTLHENADLPDTGPSSPLID
                     RKANLAGNAITLRIHENPAYFSRLLRDQLLPNMAIGLLCAIVLSTAAYLTAQRRYSIQ
                     AQLLRAIRDNEFHMEYQPIYEVSTQRCAGVEALLRWDRPAGRIGPDEFISAAEERGAI
                     IALTQHMLKLVAHDMQRWHVAPGFHTSVNISPRHIDHASFRSDMLQFFSS"
BASE COUNT          808 a         1701 c         1627 g          777 t
ORIGIN      
        1 tttcatggtg gcctatgacg gcagccccac ggcggacaaa acggtggaca tcgtcagccg
       61 cagccccctg ctggccggcc tgccggccca tctcgtgatg gtgggccgca agtccgccat
      121 ggcccaggcg ggcctggaac gggcccgcac gcaattggcg cgggccgggt ttgccgccca
      181 ggcaacgcac atcccgggcg agccgaccgc ggcgctggcc gcctatgccg agcagcatgg
      241 catcgacatg ctggccatgg gcgcctacgg acgcacgcgc ctgcgccacc tggtgctggg
      301 cagcaccacc acggccatac tgggacagct gcggctgcct ttattgattc tgcgttgaag
      361 gctgcgtcgg agtcgcagcc tggggcggcg cggcggcagg cggcgcagcg gacggcttca
      421 gcggcatggc gcgcaaggcc tgcaaggtct tcatgccggc ccggccgtcg gccggcaggc
      481 ccagttgctg ctgcaccgtc tgcagggcct ggcgcgtgcg cgcgccgatg atgccgtccg
      541 gctggccgac gtcgtagccc tgccccagca gcagggcctg cacttcgcgg cgctcggcgc
      601 gcgacaagcc gggatcgtcc gtcggccagg gcgtgacgaa cggcccgccg cccttgaggc
      661 gatcggacag gtgcgcaatg gccagggcat agctttcggc cgcgttgtac gcataaatcg
      721 catcgaaatt gcgcgtcacc aggaaggccg ggccttgcgg gccggccggc agcagcaggc
      781 ccgccatggg gtcgcccgcc ggcatgggct ggccgtcggc ccgcttgacg ccgcgcgcca
      841 cccacgacga cagggactgc ttgttgcggc ggccggcatt ggaggtgtcc agcccggccg
      901 gcaaaatcac ttcgaagccc cagggcaggc caggctgcca accggcctgc ttgaggaagt
      961 tcgccgtcga gcccagggcg tcgggcacgc tgtcgatcag gtcgcgccgg ccgtcgccgt
     1021 cgaaatccac ggccaggcgc tggtaggtcg agggcatgaa ctgcgtctgg ccgaaggccc
     1081 cggcccagga gcccatgagt ttgtcggcgg ggatgcgctc gtcctgcagg atcttgagcg
     1141 tggcgtagaa ctcgccgcga aaatacgact gccggcgccc gaagcacgac aaggtcgaca
     1201 aggaggcaag cagcggccgg ccaccctgga tgcgtccgaa gttgctctcc acgccccaca
     1261 ccgccgccac gaccgcggca tccacgccga actgggcctg cgcccggtcg aattcggcgc
     1321 gccacttggc catggcggcc aggccgtcgt ccacgcgctc ctggtcgacc aggccggcca
     1381 ggtagtccca gatgggttgc ttgaattcgg gctgcgcgtc cagaagcggc agcaccgtca
     1441 tgtcgggcga gagcagcgcg acctgggtat cgtaggtgga agccgacacg ccgtgttgcg
     1501 tggcgtcggg cttgagcgag gccaggcagc tgtggaaggt gccggcatcg gcggacgtgg
     1561 cgtcttgcgc gtaagcggtg acggacagca ggcccaaggc cagcgacagg aacgaggagc
     1621 aggtatggga cgtcatgcgg acattctagc gtccgcgtgg ttctgtattc ttaagccgcc
     1681 gaggctgccc tgcggaattc cgatgctcgc ccccactggg ggctgcgctt cggattcctc
     1741 cggggtcctc ggctcacaga gcctctaatt ccgcgtcggt gaaacccgcg gcgcggcgtg
     1801 cctccaggtt gaaggggccg cgcagcttcg gggcgccgta gtcttgcgca agcttggcat
     1861 aagcgacgat gggatcgagt ccgcgttgtt cgcatgcatg ccgataccaa tggttgccga
     1921 tcgccacatg gccgatctcg tcgcgcagga tgacgtcgag gatggcggcg gcctgcatgt
     1981 cgcccgcgcc ggccagcttg cgccggatcg gcggcgaggc gtccaggccg cgcgcctcca
     2041 gggtgcgggg caccagcgcc aggcgggcca gcaggtcgcc gcgcgttttc tcggccattt
     2101 cccacaggcc gttgtgcgcg ggaaagtcgc cgtaggcatg gcccagggtg gccaggtggc
     2161 gattgagcag gtcgaagtga tgggcctcct cgcgcgccac ccgcagccag tcgcggtaat
     2221 aggcctcggg ctgcccggca aagcgccaga tggcgtccag ggccaggttg atggcgttga
     2281 actcgatgtg cgccagggca tgcagcatgg cggcgcggcc ttgcggcgta tgcaccgggc
     2341 gcggcgccag ctggtcgggc tgcaccagct cgggccgcgc cgggcggccg ggcaggtcgg
     2401 ccggctcgtc cagaggcgcc agcgggtcga ccggcgcggt atcgtcgagg ctggccacgg
     2461 ccgccagttt ggcggggcta tccttgacct gcagggcacg cagggcctgc tcgcgcaggc
     2521 tggcggcgga gatgatggaa cgcatggaaa tcggcttgca gtcggtgcct ggagatcagg
     2581 gcctgaaatt ggtacctgaa atttagcact ccccccttta tcattgcccc atgccgccct
     2641 ccgaccactt cagcctggaa acctcgctcg aggccttcga tcgccgccaa tggaatgccc
     2701 tggcgggcga tcagcccttc gtgcggcacg agttcctgtc ggccctgcac gagacgggct
     2761 gcgccgcgcc ggacacgggc tggacgccct acttcctggc cctgcggcgc gccggcgcgc
     2821 tggccggcgc cgtgccgctc tacctgaaga cccactcgcg cggcgaatac gtgttcgact
     2881 acgcctgggc cgatgcctac gagcgccatg ggctgcgcta ctaccccaag ctgctgtcgt
     2941 ccgtgccctt cacaccggtg accggcgccc ggctgctggc cgccagccat gccgaccgcg
     3001 cggccctggc cctgggcctc atcgaactgg cgcagcagct caagctctcg tccctgcacc
     3061 tgcttttccc tcgcgacgag gacctggccg ccttgcggga agccggctac atggtgcgcg
     3121 aaggcgtgca attccactgg ctcaacccgg gctatgccgg catggacgat tttcttgccg
     3181 ccatgagcca cgacaagcgc aagaagatca agcaggaccg caagaaggtg gcgcaggccg
     3241 gcatccgcta tcgctggctg cgcggcgcgc agatcgacgc ggcgtcgctg gacttcttct
     3301 acgcatgcta ttgccgcacc tattacgaac acggcaatcc gccctacctc actgccgagt
     3361 tcttccagcg cgtgcatgaa cagatgcccg acgccatggt gctcatcctg gccatgcagg
     3421 gcgaaacgcc ggtggccgcg gcgctgaacc tgcaagacgg cgaggcgctc tatggccgct
     3481 actggggcag cacggtgttc gtctcgggcc tgcatttcga gacctgctac atgcaggcca
     3541 tcgaatactg catcgccaac cgggtggcgc gcttcgaggg cggagcgcag ggcgagcaca
     3601 aggtcgcgcg cggcctgctg cccaccccga cctggtcggc ccattggatc gccgacccgc
     3661 ggttcgccga ggcgatcgaa gacttcctga accgggaaac ggcggcaatg caggactacc
     3721 tgggggagct ggagatgcac gcgccattca agagggcatc atgactcggc aaagccacgc
     3781 ccatctcacg acccggctac cgtggcatca atacctgttg ctggccctat gcatcgtcat
     3841 ccccctgtgc gccggcgtcc tatggacatg gcatggcgcc cgccgcaacc aggaaaaaac
     3901 cgcagcggac gcggcgcaaa acatcgagca tcaactgggc atcgtcctca accgcgtccg
     3961 ccaggccttg ctgcagctga cgccgctgac cacgctgcca tgcgaacgac tcgaacccga
     4021 actcaccttg cgcacggcca cgattccccg ggtgcgcaca ttgaccgtca tggacggcca
     4081 ccggatttat tgctcctcga acggtccgct gggcacgtac aacctggaag acatgaaggg
     4141 cgcccccggc gacctgccgc cctcggactg gtccgccatc ctgggcggaa cgcccgtcgt
     4201 ccccgatcgc cccgtcctgg tcgtcgggca tccggtcccc gccagcagca agacggtgct
     4261 ggcctatgtc gacgaccgca atctgcgcga catcctgcgc gccggactgc ctccggacac
     4321 gttcctgcag accgatctca atatcggcgg cggcctcacc ctccatgaaa atgccgatct
     4381 gccggacacc gggccatcca gccctctcat cgaccgcaag gccaatctgg cgggcaacgc
     4441 aatcaccctg cgcatccatg aaaacccagc ctacttctcg cgcttgctgc gcgaccagct
     4501 attgcccaac atggcgatcg gactgctgtg cgcgattgtc ctgtccactg ccgcctactt
     4561 gacggcgcag aggcgctact cgatccaggc gcagctgctg cgcgccatcc gcgacaacga
     4621 attccacatg gaataccagc cgatctacga ggtctccacg cagcgttgcg caggcgtgga
     4681 agccctgctc cgctgggacc ggccagcagg ccggataggg cccgacgaat tcatttcggc
     4741 ggcggaagag cgcggcgcca tcatcgcgct gacccagcac atgctgaaac tcgtcgcgca
     4801 cgacatgcag cgatggcatg tcgcccccgg cttccatacc agcgtgaaca tttcaccccg
     4861 gcatatagac catgccagct ttcgatccga catgctccag tttttctcga gca
//
LOCUS       DASWPO010000284         9855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1317447, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000284 DASWPO010000000
VERSION     DASWPO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317447"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1184
                     /locus_tag="VGC69_14980"
     CDS             189..1184
                     /locus_tag="VGC69_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0296631.1"
                     /translation="MTTLITGGSGSVGLALAEHLLAAGDAVVGYDLAPPSAAAAQTFA
                     ALPGRFVAVRGDARDEAALCEAMRAHGVRHVVLMAAITANARRERAMPRQIFDVNVGG
                     VLAGLAAAAQCGVQRVLYASSGAVYGASGRDAVRDEASSALLPEGLYGMSKRAAEEAA
                     RRYSNLAGLPLVIGRLGTCFGPWEADSGMRDTLSPPLQVARLAWQGREVVLPRDSRRD
                     WLYVRDAASAIVGLLHADWRHEVYNLAAGHEWNMSQWCDRLAAKQPGFAWRYASQGDP
                     ANVDYWSDYDRGSMRISRLLADTSFVPAYDLAAAFDDFQSWIAAHGAAYLGGRAG"
     gene            1249..1779
                     /locus_tag="VGC69_14985"
     CDS             1249..1779
                     /locus_tag="VGC69_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HEY0296632.1"
                     /translation="MQGTSRALISALAASVLLALVGCGTLPKPAGQQAAAADTAPTAA
                     AAPAPAAADQVEFYVGQSQPGNGLSGLQVPGGMLYLSSTPVLTRDDLSEAAALVDKQG
                     NNFVGLRFTETGARKLNEVSTRSVGKMLALVIGRDLVASPRITEPLNKGVLAFGVPSA
                     QDAAAIAARIRGDKAQ"
     gene            complement(1876..3075)
                     /gene="gspF"
                     /locus_tag="VGC69_14990"
     CDS             complement(1876..3075)
                     /gene="gspF"
                     /locus_tag="VGC69_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY0296633.1"
                     /translation="MPSFRYEATDALGKIVRGSIEADTERGARNQLRGRGLLPLSTSS
                     SASATGSLLRPRLSDSDLAWITRHLASLLAAGLPLDGALGAALEQAEDKHMAELLASV
                     RADVRSGHRLSIALAAHPRDFPEIYRALVEAGEESGDLAQVMEKLAVYIEDRNALRSK
                     VLTAFIYPVVVACVSVVITAFLLGYVVPQVVTAFSHAHQALPLITRIMLAASDYVRHW
                     GWLTLLGLAALVTLWRYTLRAPAARLAWHSRVLRIPLAGRFVLGVNTARFASTLAILT
                     ASGVALLTSLNAARRTLSNDRLRTAVDEATARVREGAGLATALGAQRCFPALLIHLIA
                     SGEKTGRLPELLERGAANLSRDLERRAMAMTALLEPLLILVMGGIVLMIVLAVMMPIL
                     EMNQLVR"
     gene            3178..5835
                     /gene="gspD"
                     /locus_tag="VGC69_14995"
     CDS             3178..5835
                     /gene="gspD"
                     /locus_tag="VGC69_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY0296634.1"
                     /translation="MRRVTSFILAAHLLVLNMPVMAAQPDPKQQSSDQVTLNFADTDI
                     PAVLRAMSLFMKRNFVVDPRVKGKLTLMSEQPVSRNEALAMLTGALRMQGYALVEVGR
                     VTRVVPEADAKLQGSPVIDNPPAAAPLAAASPAAGQAAAQRQSDGSLESFMNASRGSE
                     IVTRVFALKYENANNLLPVLRPLVPPNNSINAYPGNNTLVITDYADNLDRIAKVIARI
                     DVPTAVDTDVVPIKSGIATDIASLASQMLDSQNSEPGQRITVVADPRSNSVVIRSSSP
                     ARSKLASDLVAKLDARQERAGGNMHVVYLRNAQAAHIAQVLGGLLTGRGQVQSVASAN
                     GGGGGGSGLANASPGGRASSAGLQSTSASSSNSANNSYGTSGGSGQGGLDSSQPISRE
                     DSSSGQPLSFAADGAVVQADPTTNSLVISAPDPLYRDLRTVIDQLDQRRAQVLVESLI
                     VEVTQDASSQFGIQWMTGGAGLSGTGTSFVSGTNLGGSGLTPGLGATTLDSLGSGLSL
                     GVIKGTTTVLGNQLINLGVLARAMQSNNQTNILSTPNLLTLDNEQASIMVGKTVPFVT
                     GQYVTTGSGSSNPFQTIQREDVGLKLNIRPQISEGGSVKLDIYQEVSSIDTASSTAAA
                     GIVTDKRAIDTSVLADDGDIIVLGGLLEDNVTLTTNSVPGLSSIPVLGALFKYDTRER
                     VKTNLMVFLRPYVLRDQNASSDLTLSRYNYMRRAQSQVQPGNHWLLPDMQASILPPAN
                     LRTNAEGAAYDMRAGHLADTLRRDPPPVSQTYAVRQLPKSLEPKPSDEPIHAILPRGV
                     GVTSDPSIMYAQEDTSTTTLQIAQVAHCEDADPIVNRIRISGLKAYSQTGPGGMSCVV
                     RTQVARDARTVDAAIELLRELGYQSEVVQ"
     gene            5859..7292
                     /gene="gspE"
                     /locus_tag="VGC69_15000"
     CDS             5859..7292
                     /gene="gspE"
                     /locus_tag="VGC69_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY0296635.1"
                     /translation="MATAPTLPYAWARMQRAILALRPGATQLTVSPRTPAWAVREVFR
                     LHGEVPVVEVDDATLENLLTASYSDTEDAASVMGVAENEIDLDRLLQDMPEVADLLEA
                     QDDAPVIRMINALFSQAARDGASDIHIEPFETHSTVRFRVDGTLRDVVSPRKALHAAL
                     VSRIKIMAHLDIAEKRLPQDGRIALRVGGRAIDVRVSTLPTGHGERAVLRLLEKEAGR
                     LQLERLGMSPPNLVKIDSLISHPHGIVLVTGPTGSGKTTTLYAALARLDAATRNILTV
                     EDPIEYDLPGIGQTQINSKIDMSFAMALRAILRQDPDVIMIGEIRDLETAQIAVQASL
                     TGHLVLATLHTNDAVSAVTRLTDMGVEPFLLASSLLGVLAQRLVRLLCPQCKRAETEP
                     DGRTVYRPVGCPSCSHTGYSGRSGIHELFTIDADLRAMIHSGATEQALRQAAVRTGMS
                     SMRDDGQRWVESGATSPEEILRVSRDD"
     gene            7285..7695
                     /locus_tag="VGC69_15005"
     CDS             7285..7695
                     /locus_tag="VGC69_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296636.1"
                     /translation="MIKIARLLAILAAAAALGVWGAILFAPRPDAPPPALGTAGVPHA
                     DVAPVALLFGRDAVLDTTVVVQGLIADGQHSAAVVSINGAAPTAVTAGLHTASGVVVR
                     AIDGQGITLDVAGSSAHAPAPARGETPAGFVAVP"
     gene            7875..>9855
                     /locus_tag="VGC69_15010"
     CDS             7875..>9855
                     /locus_tag="VGC69_15010"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY0296637.1"
                     /translation="MSMDRRNFLKWSSFLTVSVATTGLAGCNDDDNGNNGGGNNGGNG
                     GGPGGDATAYSYPQGVASGDPRPDSIVLWTRVQGGSASSIPVTVQVATDTAFKQLLVN
                     ASISATSDWDYTIRHKVTGLSPATTYYYRFLSGSATSTTGRTRTAPAAGTPVSQMKFA
                     FITCQDWSVNHWAAFTDLAAIDDLDFVVHLGDYIYETTEGTGPSPVESAHAPVTLPSG
                     GVATPNDSTGTPSASNLYANTLPDYRALYRQYRTDPRLQKLHALFPFIAIWDDHEFSD
                     DCWQDYDTYGVTPNSEQLGRRRSASQAWFEYMPADVTIDLANPSFQNITIYRSFQFGN
                     LATLVMTDERLNRVQHLVSQVAPAGSGFVGTRYFVQQTQLQAAEAGLMAAAGGALAPV
                     SMLGDTQRNWWKQQMSAATTTWKLWGNEVSLLRMGLDGTQAVAGLLFQGLLAAQPSLA
                     PVLAPSQGPIMVALVTDLATGGTTTPFTQTIAALTTVLTPVLGGPTAAGTAAAIAASF
                     TSTLASIPATLLNKYILDTDQWDGYNAERKDLMSYLQTNSIGNVVAITGDIHAFFAGP
                     VMADYDAATPVPVMVDLVTAGISSDSLFSFYKDAVDTTPGFAAAAALIYTQDATTGAV
                     TNTFNNTLTTFNPWLAGGTGYVDTDAQGYAVVTLTP"
BASE COUNT         1676 a         3353 c         3282 g         1544 t
ORIGIN      
        1 aggctgcaat gttccgccgc cctggtgaag ttgcgcgtcg tcacgagcgc ggcgaaggcc
       61 ttcatctggc gcagggtgag gttcattgct tgaatgggaa ggttgaggcc gggcaggatc
      121 ttatacatca atggaccaaa aagcaccggc cttttctttt ggtactttat gggcataggg
      181 aggctggaat gacgacgctg attacgggtg gtagcggttc tgtgggtctt gcgctggcgg
      241 agcacctgct cgccgcgggg gatgcggtgg tggggtacga cctggcgccg ccttcggccg
      301 cggccgcgca aacctttgcg gccctgcccg gccgcttcgt cgcggtgcgg ggcgatgcgc
      361 gcgacgaggc cgccttgtgc gaggccatgc gcgcgcacgg cgtcaggcac gtggtgctga
      421 tggcggccat taccgccaat gcccgccgcg aacgcgccat gccgcggcag atcttcgatg
      481 tgaacgtggg cggcgtgctg gcggggctgg ccgcggcggc gcagtgcggc gtgcagcgcg
      541 tgctgtatgc cagttccggc gctgtctatg gcgcgagcgg gcgcgatgcc gtgcgcgacg
      601 aggcaagctc ggccctgcta cccgaaggct tgtacggcat gtccaagcgc gccgccgaag
      661 aggccgcgcg gcgttattcg aacctggcgg ggctgccgct ggtcatcggc cggctgggca
      721 cctgctttgg cccctgggag gccgacagcg gcatgcgcga cacgctcagc cctccgctgc
      781 aggtggcgcg cctggcctgg cagggcaggg aagtggtcct gccgcgcgac agccggcggg
      841 attggctgta cgtgcgcgat gccgcgtcgg ccatcgtcgg cctgctgcat gccgactggc
      901 gccacgaggt ctacaacctg gcggcggggc atgagtggaa tatgtcgcaa tggtgcgatc
      961 gcctggcggc gaaacagccg ggcttcgcgt ggcgctatgc ttcgcagggc gatccggcca
     1021 atgtcgatta ttggtcggac tacgaccgcg gttcgatgcg catatcccgc ctgctggccg
     1081 atacgtcttt cgtgccggct tacgacctgg ccgcggcctt cgacgatttc cagtcctgga
     1141 tagccgcgca tggcgcggcc tatctcggcg gccgggcagg ctgaccgcgc cgtcgctgcg
     1201 agcctgtaaa cttgggcaat cgcaaagaag gaacaaggaa cgccgctcat gcaaggaacc
     1261 tcacgcgcac ttatctctgc cctggccgct tccgtgctat tggctctggt ggggtgcggc
     1321 acgctgccca agcctgccgg ccagcaggcc gcggcggcag atacggcccc cacggccgcg
     1381 gccgcgccgg cgcctgccgc cgcggaccag gtcgagttct acgtggggca atcccagccg
     1441 ggcaacggcc tgtcgggcct gcaagtgccg ggcggcatgc tttacctgtc cagcaccccg
     1501 gtcctgacgc gcgacgacct cagcgaggct gccgcgctgg tcgacaagca ggggaacaat
     1561 ttcgtgggct tgcgctttac cgagacgggc gcgcgcaagc tgaacgaggt cagcacccgc
     1621 agtgtcggca aaatgctggc gctggtcatc gggcgcgacc tggtggcctc gccgcgcatc
     1681 accgagccgc tgaacaaggg cgttctggcc ttcggcgtgc cgtcggcgca ggatgcggcc
     1741 gccatcgccg cgcgcatccg cggcgacaag gcgcagtaat ccgcaagcaa cgtcgcatac
     1801 ggcgaaaact tgccgcgagc gatggggata cggcgagccg ccggtgcgca agcgtgagcg
     1861 gttctcgtgc cgcggttagc ggacgagttg gttcatttcc aggatgggca tcatcaccgc
     1921 cagcacgatc atgaggacga tgccgcccat caccaggatg agcaggggct cgagcaaggc
     1981 cgtcatggcc atggcgcggc gctccaggtc gcgcgagaga ttggcggcgc cgcgctcgag
     2041 cagctcgggc aggcggcccg tcttttcgcc gctggcgatc aagtggatga gcaaggcggg
     2101 gaaacagcgc tgggcgccca gcgccgtcgc cagcccggcg ccttcgcgca cgcgcgcggt
     2161 cgcttcgtcg acggcggtgc gcaggcggtc gttgctcagc gtgcggcgcg cggcgttgag
     2221 ggaggtcagc aaggccaccc cgctggccgt caggatggcc agggtcgagg caaagcgcgc
     2281 cgtgttcacg cccagcacga agcgtcccgc cagcggtatg cgcagcacgc gcgagtgcca
     2341 ggccaggcgc gcggcaggcg cgcgcagggt gtagcgccat agcgtgacga gcgcggccag
     2401 ccccagcagc gtcaaccagc cccaatggcg cacatagtcg ctggccgcga gcatgatgcg
     2461 cgtgatcaag ggcagcgcct ggtgggcatg gctgaacgcc gtcacaacct gcggcacgac
     2521 ataacccagc aggaaggcgg tgatgacgac ggagacgcag gccacgacca cgggatagat
     2581 gaaggcggtc agcaccttgc tgcgcagcgc gttgcgatcc tcgatgtaga cggcgagctt
     2641 ttccatcacc tgggccaggt cgccggattc ttcgccggct tcgaccagcg cccggtagat
     2701 ctcgggaaag tcgcgcggat gcgcggccag ggcgatggaa agacgatggc cggatcgcac
     2761 atcggcgcgt accgaggcaa gcagctcggc catgtgcttg tcctcggcct gttcgagggc
     2821 ggcgccgagc gcgccgtcca gcggcaggcc ggcagccagc aggctggcga gatggcgggt
     2881 gatccatgcc aggtcgctgt cggatagacg gggacgcagc aggctgcccg tcgccgaggc
     2941 cgaggacgag gtcgagagag gcagcaggcc gcgcccgcgc agttggttgc gcgcaccgcg
     3001 ttcggtgtcg gcttcgatcg agccacgcac gatcttgccc agggcgtcgg tggcttcgta
     3061 gcgaaatgac ggcatgatgc gaccaggatc tcattctttt gttgcagtca tattgcaaaa
     3121 aatggtgcgt atactccgac gattttcgct atccacgctt tatctctgcg agacctcatg
     3181 cgccgcgtta cgtcgttcat tttggcagcc catctgctgg tactcaatat gcccgtcatg
     3241 gcggcgcagc ccgatcccaa acaacagtcc agcgatcaag tcaccctgaa tttcgccgac
     3301 accgacattc ctgccgtctt gcgggcgatg tccctgttca tgaagcgcaa cttcgtggtg
     3361 gacccgcgcg tgaaaggcaa gttgacgctg atgtccgagc aacccgtcag ccgcaacgaa
     3421 gcgctggcca tgctgacggg cgcattgcgc atgcagggct atgccttggt cgaggtgggc
     3481 agggtgacgc gcgtggttcc cgaggccgac gccaagctgc aggggagtcc ggtgatcgac
     3541 aacccgcctg ccgcggcgcc gctcgccgcg gccagtcccg cggcgggcca ggccgccgcg
     3601 cagcggcaaa gcgatggatc gctcgaatcc ttcatgaatg cctcgcgcgg cagcgagatc
     3661 gtcacgcgcg tcttcgcgct caagtatgaa aacgcgaaca acctgctccc cgtgctgcgt
     3721 ccgctggtgc cgcccaacaa cagcatcaat gcctatcccg gcaacaacac gctggtcatc
     3781 accgactatg ccgacaacct ggatcgcatc gccaaggtga tcgcgcgcat cgacgtgccg
     3841 acggcggtgg acaccgacgt ggtgccgatc aagtcgggca tcgcgacgga catcgcctcg
     3901 ctcgcctcgc agatgctgga ctcgcagaac agcgagccgg gccagcgcat caccgtggtg
     3961 gccgatccgc gcagcaacag cgtggtgatc cgctccagca gcccggcgcg cagcaagctg
     4021 gcgagcgacc tggtggccaa gctcgacgcg cgccaggagc gggcgggcgg caatatgcac
     4081 gtggtgtatt tgcgcaacgc gcaggccgcg cacatcgccc aggtgctggg cggcctgctt
     4141 accgggcgcg gccaggtgca gtccgtggcc tccgccaatg gcgggggcgg aggaggctcg
     4201 ggcctggcca atgcctcgcc gggcggccgc gcttcctccg ccgggttgca aagcacgtcc
     4261 gcgagcagca gcaacagcgc caataattcc tatggaacga gcggcggcag cggccagggc
     4321 ggcctggact cgtcccaacc catctcgcgc gaggattcca gttccgggca gcccctgtcg
     4381 ttcgcggccg atggcgctgt cgtccaggcc gacccgacca ccaactcgct ggtcatttcg
     4441 gcgcccgacc cgctttaccg cgacctgcgc acggtgatcg accagctcga tcaacgccgc
     4501 gcgcaagtgc tcgtggagag cctgatcgtg gaagtgacgc aggatgcctc ctcgcaattc
     4561 ggcatccagt ggatgaccgg cggggcgggg ctcagcggca cgggcacgtc gttcgtcagc
     4621 ggcaccaacc tgggcggcag cggcctgacc cccggtcttg gcgcgaccac cctggactcc
     4681 ctgggcagcg gcctgtcgct gggtgtgatc aagggcacga ccaccgtgct gggcaatcag
     4741 ttgatcaacc tgggcgtgct ggcgcgcgcc atgcagagca acaaccagac gaatattctt
     4801 tccacgccca atctgctgac gctggacaat gaacaggcca gcatcatggt cggcaagacc
     4861 gtgcccttcg tcaccggcca gtacgtcacc accggcagcg gcagcagcaa ccccttccag
     4921 acgatccagc gcgaggacgt gggcctcaag ctcaacatcc ggccgcagat ttccgagggc
     4981 ggatcggtca agctggacat ctatcaggaa gtcagcagca tcgacacggc cagctccacc
     5041 gccgccgcgg gcatcgtgac ggacaagcgg gccatcgaca ccagcgtcct ggccgacgac
     5101 ggcgacatca tcgtcctcgg cggcctgcta gaggacaacg tcacgctgac gaccaacagc
     5161 gtgccgggcc tgagctcgat tccggtgctg ggcgcgctgt tcaagtacga cacgcgcgag
     5221 cgcgtcaaga ccaacctgat ggtgttcctg cgcccctacg tgctgcgcga ccagaacgcc
     5281 agctccgacc tgacgctcag ccgctacaac tacatgcggc gcgcgcaaag ccaggtgcag
     5341 ccgggcaatc actggctgct gcccgacatg caggcttcca tcctgccgcc ggccaatctg
     5401 cgcaccaatg ccgagggcgc ggcctacgac atgcgcgccg gccacctggc cgacaccctg
     5461 cgccgcgatc cgccgcccgt ctcgcagacc tacgcggtgc gccagctgcc gaagtcgctg
     5521 gaacccaagc ccagcgacga gcccatccac gccatcctgc cccggggcgt gggcgtcacg
     5581 agcgatccga gcatcatgta cgcgcaagag gacaccagca cgaccaccct gcagatcgcc
     5641 caggtggctc attgcgaaga tgccgatccc atcgtcaatc gcatccgcat cagcggcctg
     5701 aaggcttatt cgcagaccgg tcccggcggg atgagctgcg tggtgcgcac ccaggtggcg
     5761 cgcgatgccc gcacggtgga tgcggccatc gagctgctgc gcgagctcgg ctaccagagc
     5821 gaggtggttc aatgaacacg ggaccggagg cctagccgat ggcgaccgca ccgaccttgc
     5881 cctatgcctg ggcgcgcatg cagcgcgcca tattggcctt gcggcccggc gcgacgcagc
     5941 tgaccgtcag cccgcgcacg ccggcctggg ccgtgcgcga ggtttttcgc ctgcacggtg
     6001 aagtgccggt ggtcgaggtg gacgacgcca cgctggaaaa tctgctgacc gcgtcctaca
     6061 gcgacaccga ggacgcggct tcggtcatgg gcgtggcgga gaacgagatc gacctggacc
     6121 gtctgctgca ggacatgccc gaggtggccg acctgctcga ggcgcaggac gatgcgccgg
     6181 tcatacgcat gatcaacgcc ttgttctcgc aggccgcgcg cgacggcgcg agcgatatcc
     6241 atatcgagcc cttcgagacg cattcgacgg tgcgctttcg cgtcgacggc acgctgcgcg
     6301 acgtggtcag cccgcgcaag gcgctgcacg cggcgctggt gtcgcgcatc aagatcatgg
     6361 cccatctcga tatcgccgag aaacgcctgc cgcaggatgg ccgcattgcc ttgcgcgtgg
     6421 gcgggcgcgc catcgacgtg cgcgtctcga cgctgcccac cggccatggc gaaagggcgg
     6481 tattgcgcct gctcgagaaa gaggcgggcc gcctgcagct cgaacgcctg ggcatgagcc
     6541 cgccgaatct ggtgaagatc gacagcctga tcagccaccc gcacggcatc gtcctggtca
     6601 cgggacccac cggcagcggc aagaccacca ccctgtatgc ggcccttgcg cggctggatg
     6661 ccgccacgcg caacatcctc acggtggagg atcccatcga atacgacctg cccggcatcg
     6721 gccagacaca gatcaacagc aagatcgaca tgagtttcgc catggcattg cgagccatat
     6781 tgcgccagga tccggacgtc atcatgatcg gcgaaatccg cgacctggaa accgcgcaga
     6841 tcgcggtgca ggcctcgctg accgggcacc tggtgctggc cacgctgcac accaatgacg
     6901 ccgtctcggc cgtgacgcgc ctgaccgaca tgggggtgga gcctttcctg ctggcctctt
     6961 cattgctggg cgtgctggcg cagcggctgg tgcgcctgct gtgcccgcag tgcaaaaggg
     7021 ccgaaaccga gcccgacgga cgcacggtct atcgccccgt gggctgcccg tcatgcagcc
     7081 atacggggta cagcgggcgt tccggcatcc atgagctttt cacgatcgac gccgacctgc
     7141 gggcgatgat acacagcggc gccaccgagc aggccctgcg ccaggcggcg gtgcgcaccg
     7201 gcatgagcag catgcgcgac gacggccagc gctgggtcga gagcggcgcc acgtcgcccg
     7261 aggaaatcct gcgggtttcg cgcgatgatt aagatcgccc gcctgcttgc catattggcc
     7321 gccgccgccg cgctgggcgt atggggcgcc atcctgttcg cgccgcggcc cgatgcgccg
     7381 ccgcccgcgc tgggcaccgc cggcgtgccg cacgccgacg tcgcgccggt tgccctgctg
     7441 ttcgggcgcg atgcggtgct ggacacgacc gtggtggtgc aaggcttgat cgccgacggg
     7501 cagcacagcg cggcggtggt cagcatcaac ggcgccgcgc cgaccgcggt caccgcgggg
     7561 ctgcacactg cctccggcgt cgtcgtccgg gccatcgacg ggcagggcat cacgctggac
     7621 gtggcgggtt catcggcgca cgcgcccgcg cccgccaggg gcgagacgcc ggccggcttc
     7681 gtcgccgttc cctgatccag ccggcaggct ggagtccgct tgcgctttca cttcacgaag
     7741 cgaggggggt tccctggctg tcatacccat gcaatcgccc agtgccctaa tagcgcggct
     7801 tttatcgtcg accggcgagc cgcgttattc cagccgataa gaattccaac cctccacttg
     7861 gccaagacaa aatcatgagc atggatcgca gaaatttcct gaagtggagc agctttctta
     7921 ccgtgtccgt cgccaccacc ggcctggcgg gttgcaatga cgacgacaac ggcaacaacg
     7981 gcggcggcaa caacggcggc aatggcggcg gtccgggagg cgatgccacc gcctattcct
     8041 acccccaggg cgttgcttca ggcgatccgc gtcccgacag catcgtgctg tggacgcgcg
     8101 tgcagggcgg cagcgcctcg tccattcccg tgaccgtgca ggtcgccacc gacacggctt
     8161 tcaagcaact gctggtcaac gccagcatca gcgccacgtc ggactgggac tacaccatcc
     8221 gccacaaggt gacgggcctg agccccgcca ccacgtacta ctaccgcttc ctgtcgggtt
     8281 cggccaccag caccacgggc cgcacgcgca ccgcgccggc cgccggcacc ccggtcagcc
     8341 agatgaagtt cgccttcatc acctgccagg actggagcgt gaaccactgg gcggcgttca
     8401 ccgacctggc cgcgatcgac gacctggatt tcgtcgtgca cctgggcgac tacatctatg
     8461 aaaccaccga aggcaccggc cccagcccgg tggaaagcgc ccacgcgccc gtcacgctgc
     8521 ccagcggcgg cgtcgccacg cccaacgact ccaccggcac ccccagcgcc agcaacctct
     8581 atgccaatac gctgccggac taccgcgccc tgtaccggca ataccgcacc gacccgcgcc
     8641 tgcagaagct gcatgcgctg ttccccttca ttgcgatctg ggacgaccac gagttctcgg
     8701 acgactgctg gcaggactac gatacctacg gcgtgacgcc caacagcgag cagctcggcc
     8761 gccgccgcag cgcgagccag gcctggttcg aatacatgcc ggccgacgtg acgatagacc
     8821 tggccaatcc ctcgttccag aacatcacga tctaccgttc gttccagttc ggcaacctgg
     8881 ccaccctggt catgaccgat gaacggctga accgcgtgca gcacctggtg tcccaggtcg
     8941 cgcctgccgg ctccggtttc gtcggcaccc gttatttcgt gcagcagacc cagttgcagg
     9001 cggcggaagc cggcctcatg gctgcggctg gcggtgccct ggcgccggtg tccatgctgg
     9061 gcgacacgca gcgcaactgg tggaagcagc agatgagcgc tgccacgacg acctggaagc
     9121 tgtggggcaa tgaggtgtcg ctgttgcgca tggggctgga tggcacgcag gccgtggcag
     9181 gcctgctgtt ccagggcttg ctcgccgcgc aaccctcgct ggcgcctgtg ctggcgccct
     9241 cgcagggtcc gatcatggtg gcccttgtca ccgatctcgc caccggcggt acgacgaccc
     9301 cgttcaccca gacgattgcc gcgctgacga ccgtgttgac gcccgtgctg ggcggcccga
     9361 cggcggcggg cacggcggcg gccattgccg cctcgttcac ctcgacgctc gcgtcgattc
     9421 ccgccacgct gctcaataaa tacattctcg acacggacca gtgggacggc tacaacgccg
     9481 aacgcaagga cctgatgagc tacctgcaga ccaacagcat cggcaacgtg gtggcgatca
     9541 ccggcgatat ccatgccttt ttcgccggcc ctgtgatggc cgactacgat gcagccacgc
     9601 ccgtgcccgt gatggtcgac ttggtgacgg cgggcatttc gagcgactcg ctgttctcct
     9661 tctacaagga cgcagtcgac accacgccgg gttttgccgc ggccgcggcc ctgatctaca
     9721 cgcaggacgc cacgacgggc gcggtgacca ataccttcaa caacacgctg acgacgttca
     9781 acccgtggct cgccggcggt accgggtatg tggacaccga cgctcaaggg tatgcggtcg
     9841 tcaccctgac gccca
//
LOCUS       DASWPO010000285         6221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_959859, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000285 DASWPO010000000
VERSION     DASWPO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959859"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1190
                     /gene="hflX"
                     /locus_tag="VGC69_15015"
     CDS             84..1190
                     /gene="hflX"
                     /locus_tag="VGC69_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0296638.1"
                     /translation="MRALIISVDLGNLDHQAHADEFAMLAEGAGAEIVGTLAARRDRP
                     DAKYFIGSGKVEEGVAMAQATLADVILFDQPLSPAQQRNLEREFNLRVVDRVALILDI
                     FALRAKSHEGKLQVELAQLQHLTTRLTRMWTHLERQRGGIGMRGPGESQLEMDKRMIG
                     SKIKVLRERLERVERQRVTQRRARARGGALSVSLVGYTNAGKSTLFNALTRAGAYAAD
                     QLFATLDTTTRRIWIEGAGSVVLSDTVGFIRDLPPNLIAAFRATLEETVHADLLLHVV
                     DAASPQREEQIAEVNKVLAEIGAAEVPTILVYNKIDKAGLEPRIERNAHGTIGRVFVS
                     AVERAGLEALRGAIAEVGQLAGSNESNFQTLQSE"
     gene            1156..2433
                     /gene="hflK"
                     /locus_tag="VGC69_15020"
     CDS             1156..2433
                     /gene="hflK"
                     /locus_tag="VGC69_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY0296639.1"
                     /translation="MSRISKLFNLNDPGWGRSNNGNGPDPNRRPRGENGPPDLDEVWR
                     DFNNKLGALFGRGRGAGNGRPGSSVPPSPRRVKIGLGLIVVVLLLLWLASGFFIVQEG
                     QVAVVTQFGKYKATASPGFQWRLPYPIQSHSLVNISQLRTFEVGFRGNARNKVLPESL
                     MLTTDENIVDMQFVVQYRLRADGAPNYLFRMRDPDDAVRQASETAMREIVGKKPMDFV
                     LYEGRTEVAAEVQTLMQQILDRYQAGIQVSTVAIQNVQPPEQVQAAFDDAVKAGQDRE
                     RQINEGQAYANQIVPMAAGQASRMTEQAEGYKAKVIGDAQGDAARFASVLGEYDKAPQ
                     TMRERLYLDTMQQVYGNSSKVMIDTRGGNNMIYLPLDKIIEQAARADAGKPASAVPAV
                     TPSSSLPAAAPPAARMPGVPSGANTLTRDRLSR"
     gene            2446..3327
                     /gene="hflC"
                     /locus_tag="VGC69_15025"
     CDS             2446..3327
                     /gene="hflC"
                     /locus_tag="VGC69_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY0296640.1"
                     /translation="MQRLLPLLVGLLIIIGLLFSCVFVVSEREYALVFSLGEVKRIIS
                     EPGLYFKLPPPLQNVVSLDKRILTIESPDAERIQTSEKKNLLIDSYVKWRIADPRLFY
                     VTFGGNERAAQERLQAQIRDALNAAVNVRTVRDVVSAERDKVMAEILTNVAKRAQPLG
                     VQVVDVRLRRIEFAPEISESVYRRMEAERTRVANELRSIGAAESEKIRADADRQREVI
                     LAQAYAKAQATMGEGDAQASSIYDAAYSKNPEFFKFYKSLEAYRTAFSKPGDLLVVDP
                     GSDFFSYFKNPQPAPAK"
     gene            3474..4625
                     /locus_tag="VGC69_15030"
     CDS             3474..4625
                     /locus_tag="VGC69_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY0296641.1"
                     /translation="MGNWLLPEGFADVLPAEARRIEELRRALLDLYRTYGFELVAPPL
                     VEYIDSLLSGTGSDLSLSTCKLVDQLSGRTLGVRADMTPQVTRIDAHLLNRAGVTRLC
                     YCGNVLHARPADLLASRELLQIGAEIYGHAGFEADLEILSLVMESLGIAGVREPRLDL
                     CHPGVLHALIESDPAAAAVGEAVTLLLREKDVTGLGELAAAGLRPDTVQALRALANLY
                     GGPEVLARARRELPPLPGLVRALDALQSLIDALPHVSMSVDLADVSGYGYHSGVTFAL
                     YAEGWHDALVRGGRYDNVSHAFGRSRPATGFSLDLRKLAAGLAPAERARAVRAPWGQE
                     PDLAEAVRALRRGGEIVVQVLPGHEHDQDEFVCDRELVLEKGAWAVRAI"
     gene            4685..5980
                     /locus_tag="VGC69_15035"
     CDS             4685..5980
                     /locus_tag="VGC69_15035"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY0296642.1"
                     /translation="MSKNVVVIGTQWGDEGKGKIVDWLAESVHGVVRFQGGHNAGHTL
                     WINGKKTILRLIPSGIMHPHVTCYIGNGVVLSPEALLKEIEELEAAGLDVRSRLKVSD
                     ICPLILPYHVAIDKAREAKKGDAKIGTTGRGIGPAYEDKVARRAIRVQDLFNPTLFDE
                     KLAELLDYHNFVLTHYLGAEAVSAEQVRDEAMKLAPAIAPMVADVSSALFAVQQQDKS
                     LLFEGAQGALLDVDHGTYPFVTSSNCVAGTASAGAGVPPQSLHYVLGITKAYTTRVGS
                     GPFPTELVDEVGNHLATIGKEFGSVTGRPRRCGWFDGAALKRSVRINGVSGLCITKLD
                     VLDGLEEIRLGVGYRVNGEFRDVLPYGAHAVAQAEPVFETLPGWSESTVGITEYGKLP
                     ANARLYLERVSEVCGVPIDLVSTGPDRNETIVLRHPLKG"
     gene            5993..>6221
                     /locus_tag="VGC69_15040"
     CDS             5993..>6221
                     /locus_tag="VGC69_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0296643.1"
                     /translation="MSLPSSTESDLWVSWDEYNHLIERLSLQVYESGWQFDVILCLAR
                     GGMRVGDVMSRIFDVPLGILATSSYREASGTK"
BASE COUNT         1060 a         2165 c         1996 g         1000 t
ORIGIN      
        1 tcgggccggg cacccaatcc gccgcgccaa tgcggcccaa gcactcaaat aacaaaccac
       61 aaagaacaaa gaaaccttca ttaatgcgcg cattgatcat cagcgtcgac ctaggcaacc
      121 tggatcacca ggcccacgcc gacgaattcg ccatgctggc agagggcgcc ggggccgaga
      181 tcgtcggcac cctggccgcc cgccgcgacc ggcccgacgc caaatacttc atcggctcgg
      241 gcaaggtcga ggagggcgtc gccatggccc aggcaacgct ggccgacgtc atcctgttcg
      301 accagccgct gtcgcccgcg cagcagcgca acctcgagcg cgagttcaac ctgcgcgtgg
      361 tcgatcgcgt ggccctgatc ctggacatct tcgccctgcg cgccaagagc cacgagggca
      421 agctgcaggt cgagctggcg caactgcagc acctgaccac gcgcctgacg cgcatgtgga
      481 cccacttgga gcggcagcgc ggcggcatcg gcatgcgcgg cccgggcgag tcccagctcg
      541 aaatggacaa gcgcatgatc ggcagcaaga tcaaggtgct gcgcgagcgg ctcgaacgtg
      601 tcgagcgcca gcgcgtcacc cagcgccgcg cccgggcgcg gggcggcgcc ttgtcggtct
      661 cgctggtggg ctacaccaac gcgggcaagt ccaccctgtt caatgccctg acgcgggccg
      721 gcgcctatgc ggccgaccaa ctgttcgcca cgctggacac caccacccgg cggatctgga
      781 tcgaaggcgc gggctcggtc gtgctgtccg atacggtggg gttcatccgc gacctgccgc
      841 ccaacctgat cgcggctttc cgcgccacgc tcgaggaaac cgtccacgcc gacctgctgc
      901 tgcacgtggt cgacgcggcc agcccgcagc gcgaggaaca gatcgccgag gtgaacaagg
      961 tgctcgccga gatcggcgcg gccgaggttc cgaccattct ggtctataac aagatcgaca
     1021 aggcggggct cgagccccgg attgaacgca atgcacatgg tacgattggc cgggtctttg
     1081 tcagcgccgt cgagcgcgcc ggtctggagg ccttgcgtgg agcaattgcc gaggtcggtc
     1141 agcttgcggg aagcaatgag tcgaatttcc aaactcttca atctgaatga tcccggctgg
     1201 ggccgcagca acaacggcaa cggccccgat cccaaccgcc ggccccgggg cgaaaatggc
     1261 ccccccgatc tcgacgaggt ctggcgcgat ttcaacaaca agctcggcgc tctgttcggc
     1321 cgaggccgcg gcgctggcaa cggccgcccg ggctcgagcg tgccgccttc gccgcggcgc
     1381 gtgaagatcg gcctcgggtt gatcgtcgtc gtgctgctgc tcctgtggct ggccagcggc
     1441 ttcttcatcg tccaggaagg ccaggttgcc gtcgtgaccc agttcggcaa gtacaaggcc
     1501 acggcctcgc ccggtttcca gtggcgcctg ccgtacccga tccagagcca cagcctggtc
     1561 aacatctcgc aactgcgcac cttcgaggtg ggctttcgcg gcaacgcgcg caacaaggtg
     1621 ctgcccgaat cgctgatgct caccaccgac gagaacatcg tcgacatgca gttcgtggtg
     1681 cagtaccgcc tgcgtgccga cggcgcgccc aactacctgt tccgcatgcg cgatccggac
     1741 gacgccgtgc gccaggcctc ggaaaccgcc atgcgcgaga tcgtgggcaa gaaacccatg
     1801 gacttcgtgc tctacgaagg ccgcaccgag gtcgccgccg aggtgcagac gctcatgcag
     1861 cagatcctcg accgctacca ggccggcatc caggtcagca cggtggccat ccagaatgtc
     1921 cagccgcccg aacaggtgca ggcggcgttc gacgacgccg tcaaggccgg gcaggaccgc
     1981 gagcgccaga tcaacgaagg ccaggcctac gccaaccaga tcgtgcccat ggccgccggc
     2041 caggcttccc gcatgaccga gcaggccgag ggctacaagg ccaaggtcat cggcgatgcg
     2101 cagggcgatg ccgcgcgctt tgcctccgtg ctcggcgaat acgacaaggc tccgcagacc
     2161 atgcgcgagc gtctctacct ggacaccatg cagcaggtct acggcaactc cagcaaggtc
     2221 atgatcgata cccgcggcgg caacaacatg atctacctgc cgctggacaa gatcatcgag
     2281 caggccgccc gcgccgatgc cggcaagccc gcatcggctg tcccggcggt cacaccgagc
     2341 agcagcctgc ccgcggccgc gccgcctgcc gcgcgcatgc cgggggtgcc cagcggcgcc
     2401 aatacgctga cccgcgatcg cctgtcgcgc taggagacct gttccatgca acgcttattg
     2461 cccctcctgg tcggcctgct catcatcatc gggctgctgt tttcctgcgt cttcgtggtc
     2521 tccgagcgcg agtacgccct ggtgttctcg ctgggcgagg tcaagcgaat catcagcgag
     2581 cccggcctgt acttcaagct gccgccgccc ctgcaaaacg tcgtctcgct cgacaagcgc
     2641 atcctcacca tcgagtcgcc cgacgccgag cgcatccaga cgtccgagaa gaagaacctg
     2701 ctcatcgact cctacgtgaa gtggcgcatc gccgatccgc ggctgttcta cgtcactttc
     2761 ggcggcaacg agcgtgccgc ccaggaacgc ctgcaggccc agatccgcga tgcgctgaac
     2821 gcggccgtca atgtgcgcac cgtgcgcgac gtggtctcgg ccgagcgcga caaggtcatg
     2881 gccgagatcc tcaccaacgt ggccaagcgc gcccagccgc tgggcgtcca ggtggtcgac
     2941 gtgcgcctgc gccgcatcga gttcgcgccc gagatttccg agtcggtcta ccgccgcatg
     3001 gaggccgagc gcacccgggt cgccaatgaa ctgcgctcca tcggcgcggc cgagagcgag
     3061 aagatccgcg ccgatgccga ccgccagcgc gaggtcatcc tggcgcaggc ctatgccaag
     3121 gcgcaggcca ccatgggcga gggcgatgcc caggccagca gcatctacga cgcggcctat
     3181 agcaagaacc ccgagttctt caagttctac aagagccttg aggcctaccg cacggccttc
     3241 tccaagcccg gcgacctgct ggtggtcgat cccggctcgg acttcttcag ctacttcaag
     3301 aacccgcagc cggcgccggc caagtagtgg ggggccgcct accggcggct ctttcccccg
     3361 atgcccaccg gttccggtgc gtgtataatg ccgcgtaagc ttttgattaa ttccgcaagc
     3421 ggctgagcgg gcttacgccc ctcagccgct tttgttttgg gtcacgtcat ttcatgggta
     3481 actggctgct gcccgaaggc ttcgccgacg ttctaccggc cgaagcccgg cgcatcgagg
     3541 aactgcgtcg cgcactgctc gacctgtatc gcacctacgg ttttgagctg gtcgcgccgc
     3601 cgctggtgga gtacatcgat tcgttgctgt cgggcaccgg cagcgacctg agtctctcca
     3661 cctgcaagct ggtcgaccag ctgtccggcc gcaccctggg ggtgcgggcc gacatgacgc
     3721 cccaagtcac ccgcatcgac gcccacctgc tcaaccgggc cggcgtcacc cggctttgct
     3781 attgcggcaa cgtgctgcat gcccgtccgg ccgacctgct ggccagccgc gaactgctgc
     3841 agatcggcgc cgaaatctac ggccatgccg gtttcgaggc cgacctcgaa atcctcagcc
     3901 tggtgatgga aagcctcggc atcgccggcg tgcgcgagcc ccggctcgac ctgtgccatc
     3961 ccggcgtgct gcatgccctg atcgagtccg atccggccgc ggcggccgtc ggcgaggccg
     4021 tcacgctgct gctgcgcgaa aaagacgtga ctggcctggg cgagctggct gccgccggcc
     4081 tgcggccgga caccgtgcag gccttgcggg cgctggccaa tttgtatggc ggccccgaag
     4141 tgctggcgcg ggcccgccgc gaactgccgc cgctgccggg actggtccgg gcgctcgacg
     4201 ccctgcagtc gctgatcgac gcgctgccgc atgtatcgat gagtgtggat ctggccgacg
     4261 tgagcggcta tggttatcac tccggcgtca ccttcgcgct gtatgccgag ggctggcacg
     4321 acgcattggt gcgcggcggc cgttacgaca acgtcagcca cgcattcggc cggtcccgtc
     4381 cggccaccgg tttcagcctc gacttgcgca agctggccgc tggcctggcg ccggccgaac
     4441 gggcccgcgc ggtgcgcgcg ccctgggggc aggagcccga cctggccgag gccgtgcgcg
     4501 ctttgcgccg cggcggcgag atcgtggtcc aggtgctgcc tggccacgaa cacgaccagg
     4561 acgaattcgt ctgcgaccgg gaactggttt tggaaaaggg cgcctgggca gtcagggcga
     4621 tttgagtctt cggcgggcgt ctgcccacct tccgagtgaa attcaacttt tatctggttt
     4681 gatcatgagc aagaacgtcg tggtgatcgg cacccaatgg ggtgacgaag gcaagggcaa
     4741 gatcgtcgac tggctggccg agtccgtgca tggggtcgtg cgcttccagg gcggccacaa
     4801 tgccgggcat acgctgtgga tcaatggcaa gaagacgatt cttcgcctga tcccctcggg
     4861 catcatgcat cctcatgtca cttgctacat cggcaatggc gtggtgctct cgcccgaggc
     4921 cctgctcaag gaaatcgaag agctcgaagc cgccggcctg gacgtgcgca gccgcctgaa
     4981 ggtctccgat atctgcccgc tgatcctgcc ctaccacgtg gccatcgaca aggcgcgcga
     5041 agccaagaag ggcgacgcca agatcggcac caccggccgc ggcatcggcc cggcctacga
     5101 agacaaggtc gcgcgccgcg ccatccgcgt gcaggatctc ttcaatccca cgctgttcga
     5161 cgagaagctc gccgagctgc tcgactatca caacttcgtg ctcacccatt acctcggcgc
     5221 cgaggcggtt tcagccgagc aggtgcgcga cgaggccatg aaactggccc cggccatcgc
     5281 gcccatggtg gccgatgtgt ccagcgccct ctttgccgtg cagcagcagg acaagagcct
     5341 gctgttcgaa ggcgcccagg gcgcgctgct agacgtcgac catggcacct atcccttcgt
     5401 caccagcagc aactgcgtgg cgggcacggc ctcggccggc gccggcgtgc cgccgcagtc
     5461 gctgcactat gtgctgggca tcaccaaggc ctacaccacg cgcgtgggtt cgggcccgtt
     5521 ccccaccgag ctggtcgacg aggtcggcaa ccacctggcc accatcggca aggaattcgg
     5581 ttcggtcacg ggccggccgc gccgctgcgg ctggttcgac ggcgcggcgc tcaagcgctc
     5641 ggtgcgcatc aacggcgtgt cgggcctgtg catcaccaag ctcgacgtgc tcgacggcct
     5701 ggaagaaatc cgcctgggcg tgggctaccg cgtcaacggc gagttccgcg acgtgctgcc
     5761 ctacggcgcg catgccgtgg cccaggccga gccggtgttc gagacgctgc ccggctggag
     5821 cgagtccacc gtcggcatta ccgaatacgg caagctgccc gccaacgcgc gcctgtatct
     5881 cgagcgggtg tccgaagtct gcggcgtgcc catcgacctg gtgtccaccg gccccgaccg
     5941 caacgaaacc atcgtcctgc gccatcccct caagggctga ggagcgcgcc gcatgagcct
     6001 gccgtccagc accgaaagcg acctgtgggt cagttgggac gaatacaacc atctcatcga
     6061 gcgcctgtcc ctgcaggtct acgagtcggg ctggcagttc gacgtcatcc tgtgcctggc
     6121 gcgcggcggc atgcgggtgg gcgatgtgat gtcgcgcatc ttcgacgtgc cgctgggcat
     6181 cctggccacc agcagctacc gcgaggcgtc gggcaccaag c
//
LOCUS       DASWPO010000286         6412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_245261, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000286 DASWPO010000000
VERSION     DASWPO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245261"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="truA"
                     /locus_tag="VGC69_15045"
     CDS             <1..693
                     /gene="truA"
                     /locus_tag="VGC69_15045"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0296644.1"
                     /translation="GTGTPVPTVCAGRTDTGVHAALQVVHLDTDLDRRDESWVRGVNA
                     FLPDSIVVQWAQTVSDDFHARFSARSRTYVYLLWRGRVRPALWAGRAGWCFRPLDLDA
                     MRQAAQALLGEHDFSSFRSAQCQAKHPVRTLHRLDIEERGPFVVFTLRANAFLHHMVR
                     NLIGALLQIGQGREPAAWMARLLQARDRRLGAPTFMADGLYLCAIEYAEGHALPALDG
                     GAALLWPFVSPR"
     gene            712..1359
                     /locus_tag="VGC69_15050"
     CDS             712..1359
                     /locus_tag="VGC69_15050"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY0296645.1"
                     /translation="MRTRVKICGLTREVDIAAAVEAGADAIGFVFHPGSKRCLTVERA
                     AQLRREVPAFVSAVALFVNASPQAVQAVVDGVQPDVLQFHGDESPDACRQAGRRYARA
                     FRVGAPGLDTADALAGHCKAYGDAAGWLFDSYSTGYGGSGLAFDHHLLSAVQADGASR
                     PLILSGGLKPESVAQAVRAVRPWAVDVSSGVELEPGIKSADRIRAFIDAVREVDA"
     gene            complement(1372..1662)
                     /locus_tag="VGC69_15055"
     CDS             complement(1372..1662)
                     /locus_tag="VGC69_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0296646.1"
                     /translation="MINEIAVIFVKEGAGAAFEAAVNKAKPLFLAAKGCHGVQLFRSI
                     EKPLEYTLVVDWETVEHHMVDFRESEAFKQWRALIGDSVAQPAQVHHVNQVM"
     gene            1840..2730
                     /locus_tag="VGC69_15060"
     CDS             1840..2730
                     /locus_tag="VGC69_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0296647.1"
                     /translation="MPVSHLLLALSVVFVWGTNFVVIKWGLADFPPFLFSTLRFVLSA
                     LPWVFFVRRPAVPFWRLAAAGIFTSVGQFDLLYWAMQRDISPGLASLAIQCQVFFTIL
                     LAIILGGERMKVLQVGAMLLAVLGMAVVGWHGARDPSSTITLFGLLIVLVAAFCWACA
                     NTLVRGAGKVDMLGFTMWSSLYAVPPLILVSLWAEGPARVTQALAQASWQGWVAVLWQ
                     ALGNTVFGFGAWNWLLARHPAATVTPVALLVPVWGMISSAWLLAEPLPGWKLVAALLV
                     MAGLTLNLYAGRLQPKAAAA"
     gene            complement(2757..5150)
                     /locus_tag="VGC69_15065"
     CDS             complement(2757..5150)
                     /locus_tag="VGC69_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HEY0296648.1"
                     /translation="MTSFIHHPGRRLRAGLLACFFAFFAVLGGATAWAQPNGNPSDTT
                     DAQLTQTREKLDTIRKGLENQPSDEALAQWGADVLQLQSKADALAEALTPRLADIAAR
                     LNQLGPVPAGTKEPRDVADQRAQLVKANSAVDSQLKLAKLLSVEAAQLTERVSTERRT
                     QFQAQMGERHRTIFSSTYRAELRRDLPADLQHFGELGGELAASAGQTPGIVWFLVVVG
                     IAAIVAARYGIRRLLLHVASTRVPSGRLRRSVFAAAMVLVAVATPGLIAQLLHWGLNW
                     NDTLPDADEELAGALVGIICLAGYIGGLGHALMSPKRPSWRLLPLPDNVATGLRWLPG
                     TLAVLAVIVWLTQQLPGMLDISLTLTIAMTSLAAVALGVALAWGLTLGEQLRRQSQRD
                     PDTGHVLHRPFWISVLSTLCWLALIVGVVSILIGYAALGTFIITQGLWLLIVASSAYL
                     LSLLINDGFMTLLAGKKLDHPTPHSRLRHQTAVLLSGAGRVVVVIVAIVLLLNPFGGG
                     PTEALHSLDQFRKGLRVGEVTIQPDALWQGLLVLVLGLLCVRLLKRWLKETYLPSTTM
                     DPGMRTSTSTLFGYAGVIFVAALALSALGLGLERVAWIASALSVGIGFGLQAVVQNFV
                     SGLILLAERPVKVGDWVSLGGVEGDIQRINVRATEILMQDKSTVIVPNSEFITKTVRN
                     VTHSRSMGRVQIKLPMPLETDADKVRELVTAVFAEQEEILEAPAATVYLDGIENDRLM
                     FNALAYVESPRLAYVVRSRVLYEVLSRLAKAELQISASSTVVLQGNAGLPPAAEPQP"
     gene            complement(5257..5763)
                     /locus_tag="VGC69_15070"
     CDS             complement(5257..5763)
                     /locus_tag="VGC69_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296649.1"
                     /translation="MLFSHGKHWIYHWEITMKRACLALLALFFAASAFAGEDPEGDKI
                     MHAYVLAMPKVQAYDAATVALYDAQKKDPAFKAESDAMAREPSDTVAQLRSKMDRHPR
                     IFSFFQSRGLTRDDAALMPFVLLDACSVAQAPNPAPDPSISAQQIAFCKANQTALQNL
                     KMFSNKLE"
BASE COUNT         1065 a         2104 c         2063 g         1180 t
ORIGIN      
        1 ggtacaggca cgccggtgcc cacggtctgc gcgggccgca ccgacaccgg cgtgcacgcc
       61 gccctgcaag tcgtgcatct cgacacggac ctggaccgcc gcgacgaatc ctgggtgcgg
      121 ggcgtcaatg ccttcctgcc cgacagcatc gtcgtacagt gggcgcaaac cgtgtccgac
      181 gattttcatg cccgcttctc ggcccgctcg cgcacctacg tctacctgct gtggcgcggc
      241 cgggtaaggc cggccttgtg ggctggccgc gccggctggt gcttccggcc gctcgatctc
      301 gatgccatgc ggcaggcggc ccaggccttg ctgggcgaac acgacttctc cagctttcgc
      361 tcggcccaat gccaggcgaa gcaccccgtg cggactttgc atcgcctcga catcgaagaa
      421 cgtggtccct tcgtcgtctt caccctgcgg gccaatgcct ttctgcacca catggtgcgc
      481 aacctcatcg gcgccttgct gcagataggg caggggcgcg agcccgcggc atggatggcg
      541 cggctgctgc aggcccgcga ccgccgcctc ggcgctccca ccttcatggc cgacggtctc
      601 tatctctgcg ccatcgagta cgctgaaggc catgctttgc cggcgctcga cggcggtgcc
      661 gctttattgt ggccctttgt ttcgcctcgt taattcgctc caataaccgc catgcgtacc
      721 cgcgtcaaaa tctgcggcct cactcgcgag gtcgatatcg ccgccgccgt cgaagcggga
      781 gccgatgcca tcggctttgt ctttcatccc ggcagcaagc gttgcctcac ggtcgaacgc
      841 gcggcgcaat tgcgccgcga ggtgcctgcc ttcgtgagcg cggtcgccct gttcgtcaac
      901 gcctcgcccc aggccgtcca ggcggtggtc gacggcgtcc agcccgacgt gctgcagttt
      961 catggcgatg agtcgcccga cgcctgccgg caggctggcc gccgctacgc gcgagccttc
     1021 cgcgtcggcg cgccgggcct ggatacggcc gatgccttgg ccggacactg caaggcttat
     1081 ggcgatgccg ctggctggtt gttcgacagc tacagcacgg gctatggcgg cagcgggctg
     1141 gctttcgacc atcacctgct gtcggccgtg caggccgatg gcgcatcgcg cccgctcatc
     1201 ctctcgggcg gcctcaagcc ggagagcgtg gcccaggccg tgcgcgccgt gcgcccctgg
     1261 gccgtggatg tcagcagcgg cgtcgagctc gaacccggca tcaagtccgc cgaccgtatc
     1321 cgcgccttca tcgacgccgt gcgcgaggtc gatgcctgat cttcaggcgc cttacatcac
     1381 ctggttcacg tgatgcactt gcgccggctg cgcgacgctg tcgccgatca gggcgcgcca
     1441 ctgcttgaag gcttccgatt cgcggaagtc caccatatgg tgctccaccg tttcccagtc
     1501 gacgaccagg gtgtattcca gcggtttttc aatgctgcgg aaaagctgca cgccatggca
     1561 gcccttggcg gccaggaaca ggggcttggc cttgttcacg gccgcctcga aggccgcgcc
     1621 ggcgccttcc ttgacgaaaa tgacggcgat ttcgttgatc atgctgtgtc tccagggcag
     1681 ggttagggca agccccaggc aggcgccggg cttgcatcga gtttgccagt atcgcgcatc
     1741 tggcctggtg ccgtcgatgc gaagtgtcag gccgccgccc gcgggttttt cgataaactt
     1801 ggactcctgc agtcatcatc gggccagttg cccagaatca tgccggtttc ccatttgctc
     1861 ttggccctga gtgtcgtctt tgtctggggc acgaatttcg tcgtcatcaa atgggggctg
     1921 gccgatttcc cgcctttcct gttctcgacg ctgcgcttcg tcctctcggc cttgccctgg
     1981 gtgttcttcg tgcggcgtcc ggccgttcct ttctggcgcc tggccgcggc cggcatcttc
     2041 accagcgtgg gccagttcga cctcctgtac tgggccatgc agcgcgacat ctcgcccggc
     2101 ctggcctcgc tggccatcca gtgccaggtg ttcttcacca tcctgctggc catcatcctc
     2161 ggcggcgaac gcatgaaagt cctgcaggtc ggcgccatgc tgctggccgt gctcggcatg
     2221 gccgtcgtgg gctggcatgg cgcgcgcgac cccagcagca ccattacctt gttcggcttg
     2281 ctcatcgtgc tggtggcggc attttgctgg gcctgcgcca acacgttggt acgcggcgca
     2341 ggcaaggtcg acatgctggg cttcaccatg tggagcagcc tctatgccgt gccgcccctg
     2401 atcctggtca gcctctgggc cgaaggcccg gccagggtga cgcaggccct ggcgcaagcc
     2461 agttggcagg gctgggtagc cgtgctgtgg caggccctgg gcaatacggt gttcggcttc
     2521 ggcgcctgga actggctgct ggcgcgccat ccggccgcca cggtcacgcc cgttgccttg
     2581 ctggtgcccg tctggggcat gatctcgtcg gcctggctgc tggccgaacc cctgccgggc
     2641 tggaagctcg tcgccgccct gctggtgatg gccggcctca cgctcaattt gtatgcaggc
     2701 cgcctgcaac ccaaggctgc ggcggcctga tatcgggcaa tcggcccgaa ccgccgtcag
     2761 ggttggggct ccgcggccgg cggcaagccg gcattgcctt gcagcaccac ggtcgacgag
     2821 gccgagatct gcagctccgc cttggcaagg cggctcagca cctcgtagag cacgcggctg
     2881 cgcacgacat aggccagcct gggcgattcc acataggcca gcgcgttgaa catcagccgg
     2941 tcgttctcga tgccgtccag atagactgtc gccgcggggg cttccagaat ttcttcctgc
     3001 tcggcgaaga cggccgtgac cagttcgcgc accttatcgg catcggtttc cagcggcatg
     3061 ggcagcttga tctgcacgcg gcccatcgat ctcgagtgcg tcacgttgcg cacggtcttg
     3121 gtgatgaact ccgagttcgg cacgatcacc gtggacttgt cctgcatcag gatttccgtg
     3181 gcgcgcacgt tgatgcgctg gatgtcgcct tccaccccgc ccagcgacac ccagtcgcca
     3241 acctttaccg gccgctcggc tagcaggatc aggcccgaca cgaagttctg caccaccgcc
     3301 tgcaggccga aaccgatacc caccgacaac gcgctggcga tccaggccac gcgctccagt
     3361 cccagtccca gggccgagag cgccagggcc gccacgaaga tcacgccggc atagccgaac
     3421 agcgtcgagg tcgaggtgcg catgcccgga tccatcgtcg tggagggcag gtaggtctct
     3481 ttcaaccagc gcttgagcag ccgcacgcac agcaggccca gcaccagcac cagcaagccc
     3541 tgccacaggg catcgggctg tatcgtcacc tcgccgaccc tcaggccctt gcggaactgg
     3601 tccaggctgt gcagcgcttc ggtcggcccg ccgccgaaag ggttgagcag cagcacgatg
     3661 gcaactatca ccacgaccac gcggcccgcg cccgacagca gcacggcggt ttggtggcgc
     3721 aggcgcgagt ggggggtagg gtgatcgagc ttcttgcccg ccagcagcgt catgaagccg
     3781 tcgttgatca gcagcgacag cagataggcc gacgaggcca cgatcagcag ccacagccct
     3841 tgcgtgatga tgaaggtgcc caacgcggca tagccgatca gaatgctcac cacgcccacg
     3901 atcagggcca gccagcacag ggtgctcagc acggagatcc agaaggggcg gtgcagcaca
     3961 tggccggtat cggggtcgcg ctgcgattgg cggcgcaact gctcgcccag cgtcaggccc
     4021 cacgccagcg cgacgcccag ggcgacggcg gccaggctgg tcatggctat cgtgagggtc
     4081 aggctgatgt ccagcatgcc gggcagttgc tgcgtcagcc acacgatcac ggccagcacg
     4141 gccagcgtgc cgggcagcca gcgcaggccc gtcgccacat tgtccggcag cggcagcagg
     4201 cgccacgaag gccgcttggg cgacatcagc gcatgaccca gtccgccgat gtagccggcc
     4261 aggcagatga tgcccaccag ggcgccggcc agctcctcat cggcgtccgg cagcgtatcg
     4321 ttccagttca atccccaatg cagcaactgg gcgatcaagc cgggcgtggc caccgccacc
     4381 aataccatcg cggcggcaaa caccgagcgg cgcagccgcc ccgaaggcac gcgggtcgac
     4441 gccacatgca gcagcaggcg ccgtatgccg tagcgcgccg cgacgatggc ggcaatgccg
     4501 acgacgacca ggaaccacac gatgccgggc gtctggccgg cgctggcggc cagttcaccg
     4561 cccaattcgc cgaagtgctg caggtcggcg ggcaggtcgc gccgcagttc ggcgcggtaa
     4621 gtgctcgaga agatggtgcg gtggcgctcg cccatctgcg cctggaactg cgtgcgccgt
     4681 tccgtcgaca cacgctcggt caactgcgcc gcttccaccg acaacagctt ggccagcttg
     4741 agctgcgagt ccaccgcact gttggccttg accaattggg cgcgctggtc ggcaacatcg
     4801 cgcggctcct tggtgccggc cggcacgggg cccagctggt tcagccgtgc cgcgatgtca
     4861 gccagccgcg gcgtcagcgc ttcggccagg gcatcggcct tgctctgcag ttgcaggaca
     4921 tcggcgcccc attgggccag cgcttcatcg ctgggttggt tctccaggcc cttgcggatg
     4981 gtatcgagct tctcgcgggt ctgcgtcagt tgcgcatcgg tggtgtccga tggattgccg
     5041 ttgggttgcg cccaggccgt ggcgccgccg agcacggcaa aaaaagcaaa aaagcaagcc
     5101 agcagcccgg cgcgcagccg gcgccccggg tgatgaatga aagaggtcat gaacacatct
     5161 atgttttatc cgagctggca gcatagcaag cgccggctcg ggggtttgcg acaaaatcta
     5221 tcgtggattt tgcgcaggca gccgtctggc atgacgctac tcgagcttgt tcgagaacat
     5281 cttcaggttt tgcagcgcgg tttgattggc cttgcagaag gcaatttgct gcgccgaaat
     5341 gctcggatcg ggcgcggggt tcggcgcctg cgcgaccgaa caggcatcca gcagcacaaa
     5401 gggcatgagg gccgcatcat cccgggtcag cccccggctc tggaaaaagc tgaaaatcct
     5461 gggatgccgg tccatcttgc tgcgcagctg cgccacggtg tccgagggtt cgcgggccat
     5521 ggcgtcgctt tccgccttga aggccgggtc tttcttctgg gcgtcataca gtgcaaccgt
     5581 cgccgcgtcg taagcttgca ccttgggcat ggccagcaca taggcatgca tgatcttgtc
     5641 gccctcggga tcttcacccg caaaggcgga tgctgcaaaa aagagggcca gcaaggccag
     5701 acaagctcgt ttcattgtta tctcccaatg gtaaatccag tgcttcccgt gggaaaaaag
     5761 catagcaccg gcacctgtca aaacccccgc atggggcaga tggaagatcc agccagccgc
     5821 tgcagcgagt ttcaggcaag gccttgcctg ccgtgttgtt ctggccggcg atttgcctcc
     5881 gataaaaggg acgtgctata tttacaggct cggcagttgc aacgtaatta gttttacgca
     5941 ggtggttttg acgcaacggc cgagggcgag agagcaggcc agcgggtcgg ttgagcgggg
     6001 cagaagttac ccgggtttga cagtctcaaa aaagcggttc ataatctcgt ttctctgctt
     6061 ctgacgcagc gaacaggttg ggcgaaagtt cagcgggttt ggcggcgcga agcgggcaag
     6121 aagaggggag atgggcgaag tcagctggtc tttccgaatt ttgcgcagta cctgttcttt
     6181 aacaacgaaa caaccgataa gtgtgggcac ttggcgtgcg agtcaggtga cgtaggcctg
     6241 ttcctttctg aggggcggga gcgtcaccgc aagcaaaaag ctaagtgctc acaagtgaag
     6301 caaggtaagg ggataacccc cgggtgcaaa cccaggggat caaacccccg gggctcaggc
     6361 ctcgaaatta tcttgtcttc ctttgagaag ctagcgcgcc tccggtgata gc
//
LOCUS       DASWPO010000287        11263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2745892, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000287 DASWPO010000000
VERSION     DASWPO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2745892"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4021)
                     /locus_tag="VGC69_15075"
     CDS             complement(<1..4021)
                     /locus_tag="VGC69_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY0296650.1"
                     /translation="MVKRVAVVGMSFRFPGTDTQRYWNDLLEGKDLVTQVEAGRWSHD
                     AYLHPGKEHPGTAYTFAAGSIGDISGFDADFFGISPREASLMDPQQRLLLELGWETIE
                     SAGIKPSALRGSDCGVYIGIASADYSYRLADDMAAMDSSTATGNTASIAANRLSYVFD
                     LRGPSMAVDTACSSAMVAFHQACRAIASGDISQALAGGVSLHLHPYGFIVFSKASMLS
                     PNGRCRVFDASGDGYVRSEGGGLFLLKDYDRAVADGDPILAVVANTVVNTDGRKPGLT
                     VPSADAQATLMRRAYEQAGISPADIDYLEAHGTGTPVGDPIETHAIGLALGQARGADA
                     PLPIGSIKSNLGHLEAASAVAGLVKAIYSLRHRTVPATIGIEQLNPHIHFDDWNIRVV
                     TQPQRLRATGTLTIGVNSFGFGGANAHTILQTPPEQTVPQTPSLMGTTAPLPIVVSGR
                     NSAALRDAARDMAHALREQPARTLYDMAYHAAMRREWHDERAVVFGTDSEQIAGILDQ
                     LAEDSAPKELVQTASRLADAQGPVFVYSGNGSQWPGMGRKLLANPVFAEAIDEVDDVF
                     RALSGWSLADRLQHAESADAYEHTEVAQPALFAIQVGVTRMLAQRGVQPLAVVGHSVG
                     EVAAAWASGALTLTDAVKVIYHRSQMQGRTKGLGRMAAVGVDGGAALALLAELGLQGQ
                     VCVAGYNSPNGATLAGDPAALEQVGQALAERRAFYKQLDLDYAFHSPAMDGIEAPLCA
                     ALDDLRPQPATIPFYSAVSGGVIDGATLDAGYWWRNIRQPVRFEQAMSALVADGNNVF
                     VEIGPAPVLRSYASDALKHAEKTGRVIPTGHRGDDAPARVLAAAGQVLASGAGINWAR
                     LFPWVGNHIDLPAYPWQRERCWHPMTSEALARLERRNAHALLGHALRQQAQPTWENQL
                     DTQLQPWLADHVVGNATVFPGSGFAEIALAAAQQWQPGAGADIEELEILAPLLLAASP
                     SKITRLHLDESDGRFWLRGKETGSTDPWMLHASGRILTHARPEQLDEAAPPLPGRRPD
                     FTGDSHLALTRKAELAYGPAFQAVSHGWMASPDHVLAVLQAPAALADSLPGSLLHPAL
                     LDCTFQLIIQMLKDDAFLGQGIAFVPAKIGRLRWQAGLGAPHLVSARLLKRSPHSVTA
                     EFSLFDEQGRQIAHVGEARFRAARLAKARTDTLDFLDYAAVPSPHPLSPATANVALQS
                     KTLQPALQAVAQAVQDDELMARYAHEVDPLLENLCDRYAAQALYALSRKGHLGAGTLA
                     DLGKAAPQAAALLDWLLQRAAHLRADRLNWQVDAPQADEASTLDIWNSLLREYPDYFA
                     IVHAAGRVGLHLQALLTGQA"
     gene            4020..4250
                     /locus_tag="VGC69_15080"
     CDS             4020..4250
                     /locus_tag="VGC69_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296651.1"
                     /translation="MSNFLPGYDNNLAGFPLCSNRDASVPRVWRRPGGNMQQKKIVLG
                     GKRRLTPKLRFLFWALGLGHELHDFSGNGNQI"
     gene            4817..5878
                     /gene="rfbB"
                     /locus_tag="VGC69_15085"
     CDS             4817..5878
                     /gene="rfbB"
                     /locus_tag="VGC69_15085"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY0296652.1"
                     /translation="MTLLVTGGAGFIGANFVLDWFNQSGETLINLDKLTYAGNPDTLA
                     SLQGDARHVLVQGDIGDSQLVAGLLAKHQPRAVVNFAAESHVDRSIHGPAEFMQTNVM
                     GTFNLLESVRAYLPTLQPEARQAFRFLHVSTDEVYGTLAPQDPPFTEENQYAPNSPYS
                     ASKAASDHLVRAWHHTYGLPVLTTNCSNNYGPYHFPEKLIPLIIHNALAGKPLPVYGD
                     GMQVRDWLYVKDHCSAIRRVLEAGALGETYNVGGWNERANIDVVRTICGLLDELRPLE
                     AGRHYSDLITYVKDRPGHDRRYAIDAHRIERELGWRPAETFETGIRKTVQWYLDNQAW
                     VQGVTSGAYRNWVSRQYGT"
     gene            5891..6790
                     /gene="rfbD"
                     /locus_tag="VGC69_15090"
     CDS             5891..6790
                     /gene="rfbD"
                     /locus_tag="VGC69_15090"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY0296653.1"
                     /translation="MKILITGCKGQVGFELQRSLAVLGDVVPVDRSRCDLADPQSICE
                     VMRAERPDIVVNAAAYTAVDKAEQEQDLAAQVNGRGPGVLAAEAESLGALLVHYSTDY
                     VFDGLQHTPYSERDVTSPAGAYGRTKLEGEHAVRTATKRYLILRTSWVYGVHGGNFLK
                     TMLRVMRERDALRVVGDQVGAPTGAALIADVTAHLLSKYLAARGEPFPYGTYHLAAKG
                     ETTWYDYAGLIGRQAIEAGVPLKVQPEQIQCITTEDYPLPARRPAFSRLDTTKLRETF
                     GLVLPPWEDGVKHALKVLLPGAA"
     gene            6880..7707
                     /locus_tag="VGC69_15095"
     CDS             6880..7707
                     /locus_tag="VGC69_15095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEY0296654.1"
                     /translation="MAEALRFFPDGRVGGHLMADMLSWRLEEDGLPCFLGPGGEPAIR
                     TTILPGGGGWLQASSVRTGSEVFRLKQHDFGDRKRWSALTRNHYANEIATYGWEIGDH
                     SYGVPQVFERMAKLKIGKYVSIAEGVRIALGNHCMDNATTYPFAALSLWWPSAPTGAE
                     DDHATRGDVVIGHDVWIAAGVFIASGVTIGNGAVIGAAAVVTRDVPPYSVVAGNPARV
                     TRYRFDARIIAELQALAWWHWPDEVVDRFLPLMLQRDLQAFIDAARAQGLGPKAESA"
     gene            complement(7772..8851)
                     /locus_tag="VGC69_15100"
     CDS             complement(7772..8851)
                     /locus_tag="VGC69_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296655.1"
                     /translation="MASIALQAHALHPFLEATLRSVEAERRHRRYVHELEPRLENLID
                     RHARHVLYRLSRQGLLSAETLDACRQASPETAPFLGWLLARLARPQPEGNWLIARPQF
                     DRHSLDYTWSNLLREQPEYLPIVHACGRIDLYLNHILTGSRPVDEAYPQDLSATALYR
                     HVGNETATHGVADVLVDRISQARLMGTAEQHIGLLEIGPFGPVFGAELREALQANRCD
                     YTYLSPSDTAAATVPQADLILFHADYAASQPAVRALGFASRHLKPGGTLLLRGLQPAA
                     WMDFLFGARAGWWVDGLDGGKASPQSPPATWQVQMIQLGLMCCEDPLEYAPGTGRGAY
                     QLMATLPASRSAVAAAPRKNAQTLD"
     gene            complement(9179..10270)
                     /locus_tag="VGC69_15105"
     CDS             complement(9179..10270)
                     /locus_tag="VGC69_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEY0296656.1"
                     /translation="MATLSLKQVKKTYAGNVAVIHGIDMEIADGEFIVIVGPSGCGKS
                     TLMRMVAGLETVTSGEIQIDGVTVNDREPAERDIAMVFQNYALYPHMSVFDNMAYGLK
                     LRKLSKEDIKKRVEDAAQILELSKLLERRPRALSGGQRQRVAMGRAIVREPKVFLFDE
                     PLSNLDAKLRVAMRLEILKLHRRLKTTSLYVTHDQVEAMTLAHRMVVMNLGVPEQIGT
                     PIEVFEKPASVFVAGFIGSPPMNLIPVQVAGDGTLQTAEGSLPLDIPPTDVPPGVRGR
                     KVVMGLRPEYMMLNTRGLAAEVEMIETLGSEQLVHCRYGNAQIVVRCTTRQLTELQVQ
                     PGARLEIGPDGRHPLHWFEADTGKRVAGL"
     gene            complement(10295..11134)
                     /gene="ugpE"
                     /locus_tag="VGC69_15110"
     CDS             complement(10295..11134)
                     /gene="ugpE"
                     /locus_tag="VGC69_15110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEY0296657.1"
                     /translation="MVERRPWLDFLAHAILLAGLAIVAFPLYVTFIASTQTAQEVANA
                     PMSLLPGSHLVDNYLHVLSSGSSGTHVGRMLWVSLVMALSIAIGKILISIISAYAVVY
                     FRFPGRKLFFWMIFITLMLPVEVRIAPTYKVASDLGLLNSYAGLALPLIASATATFLF
                     RQFFLTIPDELVEAARIDGAGPIRFFRDVLLPLSKTSIAALFVIQFIYGWNQYLWPLL
                     VTTKEDMYPIVVGIRRMISGGDSVTEWNLTMATAMLAMIPPVLVVIIMQRWFVQGLVD
                     SEK"
     gene            complement(11154..>11263)
                     /locus_tag="VGC69_15115"
     CDS             complement(11154..>11263)
                     /locus_tag="VGC69_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417568.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
                     /protein_id="HEY0296658.1"
                     /translation="GLDLGSSAAQSVVLMVIVVLLTIAQFRYIDRKVQY"
BASE COUNT         1799 a         3415 c         4108 g         1941 t
ORIGIN      
        1 cggcctggcc ggtcagcagg gcctgcaagt gcaggcccac gcggccggcg gcatgcacga
       61 tggcgaagta atcggggtat tcgcgcagca ggctgttcca gatgtcgagc gtgctggctt
      121 cgtcggcctg cggggcgtcg acctgccagt tcagccggtc ggcgcgcagg tgggcggcgc
      181 gctggagcag ccagtcgagc agcgccgccg cttgcggggc ggccttgccc aggtcggcca
      241 gggtgccggc gccgagatgg cccttgcgcg acagggcgta gagcgcctgc gcggcatagc
      301 ggtcgcacag gttttccagc agcgggtcga cttcatgggc gtagcgcgcc atcagttcgt
      361 cgtcctgcac ggcttgcgcg acggcctgca gcgcgggctg cagggtcttg ctctgcaggg
      421 ccacgttggc ggtggcgggc gagagcgggt gcgggctggg cacggccgcg taatcgagaa
      481 agtccagcgt atcggtgcgg gccttggcca ggcgcgcggc gcgaaagcgc gcttcgccga
      541 cgtgggcgat ttgccggcct tgctcgtcga acaggctgaa ctcggcggtg accgaatgcg
      601 gcgaacgctt cagcagccgg gcgctgacca ggtgcggcgc gcccaggccg gcttgccagc
      661 gcaggcggcc gatcttggcg ggcacgaagg caatgccctg gcccaggaag gcatcgtcct
      721 tgagcatctg gatgatgagc tggaaggtgc agtccagcag ggcggggtgc agcaggctgc
      781 ccggcaggct gtcggccagg gccgcgggcg cctgcagcac ggccagcacg tggtcgggcg
      841 acgccatcca gccgtgcgag acggcctgga aggccgggcc gtaggccagt tccgccttgc
      901 gcgtgagcgc caggtggctg tcgccggtga agtcggggcg ccggccgggc agcggcggcg
      961 cggcttcgtc gagctgttcg gggcgggcgt gggtgaggat ccggccgctg gcatgcagca
     1021 tccagggatc ggtgctgccg gtttccttgc cgcgcagcca gaagcggccg tcgctttcgt
     1081 ccaggtgcag gcgggtgatc ttgctgggcg aggcggccag cagcagcggc gcgaggatct
     1141 ccagctcttc gatgtcggcg ccggcgccgg gttgccattg ctgggcggcg gccagggcga
     1201 tttcggcaaa gcccgagccg ggaaacaccg tggcattgcc cacgacgtgg tcggccagcc
     1261 agggctgcag ctgcgtgtcg agctggtttt cccaggtggg ctgcgcttgc tggcgcagcg
     1321 cgtggcccag gagggcgtgc gcgttgcggc gttcgagccg ggccagggcc tcggacgtca
     1381 tggggtgcca gcagcgttcg cgttgccagg ggtaggcggg caggtcgatg tggttgccca
     1441 cccaggggaa gaggcgcgcc cagttgatgc ccgcgccgct ggccagcacc tggccggcgg
     1501 cggccagcac gcgggccggc gcgtcgtcgc cgcgatggcc ggtggggatc acgcggccgg
     1561 ttttttcggc gtgcttgagc gcgtcgctgg catagctgcg caacaccggc gcggggccga
     1621 tttccacgaa gacgttattg ccgtccgcca ccagggccga catggcctgc tcgaagcgca
     1681 cgggttggcg gatattgcgc caccagtagc cggcgtcgag cgtggcgccg tcgatgacgc
     1741 cgccgctgac ggcggaatag aaggggatcg tggccggctg cgggcgcagg tcgtccaggg
     1801 ccgcgcacag cggcgcttcg atgccgtcca tggccgggct gtggaaggcg tagtccaggt
     1861 cgagctgctt gtagaaggcg cggcgttcgg ccagcgcctg gccgacctgt tccagcgcgg
     1921 ccgggtcgcc ggcgagcgtc gccccgttcg ggctgttgta gccggccacg cacacttggc
     1981 cttgcaggcc cagctcggcc agcagcgcca gggcggcgcc gccgtccacg cccacggcgg
     2041 ccatgcggcc caggcctttg gtgcggccct gcatctggct gcggtggtag atgaccttga
     2101 cggcgtcggt cagcgtcagc gcgccgctgg cccaggcggc cgcgacttcg cccacgctgt
     2161 ggccgaccac ggccaggggt tgcacgccgc gctgggccag catgcgcgtc acgcccacct
     2221 ggatggcgaa gagcgcgggc tgggcgacct cggtgtgttc gtaggcgtcg gcggactcgg
     2281 cgtgctggag cctgtcggcc agcgaccagc ccgacagcgc gcgaaacacg tcatccactt
     2341 cgtcgatggc ttcggcgaac acggggttgg ccagcagctt gcggcccatg cccggccact
     2401 gcgaaccgtt gcccgaatac acgaagaccg ggccctgggc gtcggccagg cgggacgcgg
     2461 tctgcaccag ttctttcggg gcgctgtcct cggcgagctg gtcgagaatg ccggcgatct
     2521 gttcgctgtc ggtgccgaag accacggcgc gctcgtcgtg ccattcgcgc cgcatggcgg
     2581 cgtggtaggc catgtcgtac agggtgcggg ccggctgttc gcgcagcgca tgggccatgt
     2641 cgcgcgcggc gtcgcgcagg gcggcgctgt tgcggccgct gaccacgatg ggcagggggg
     2701 cggtggtgcc catcaggctg ggcgtctgcg ggacggtctg ctcgggcggg gtctgcagga
     2761 tggtgtgggc gttggccccg ccaaagccga aggagttcac gccgatggtg agcgtgccgg
     2821 tggcgcgcag ccgctggggc tgcgtcacca cccggatgtt ccagtcgtcg aagtggatgt
     2881 gggggttgag ctgctcgatg ccgatggtgg ccggcacggt gcgatggcgc aggctgtaga
     2941 tggccttgac cagcccggcc acggccgagg cggcctccag gtggccgagg ttgctcttga
     3001 tggagccgat gggcagcggc gcatcggcac cgcgggcctg gcccagcgcc aggccgatgg
     3061 cgtgcgtctc gatggggtcg cccaccgggg tgccggtgcc gtgggcttcc aggtagtcga
     3121 tgtcggcggg cgagatgccg gcctgttcgt aggcccggcg catcagcgtg gcctgcgcgt
     3181 cggcgctggg cacggtcagg cccggcttgc ggccgtcggt gttgacgacg gtattggcca
     3241 ccacggccag gatggggtcg ccgtcggcca cggcccggtc gtagtccttc agcaggaaga
     3301 gaccgccgcc ttccgagcgc acatagccgt cgcccgaggc gtcgaacacc cggcagcggc
     3361 cgttgggcga cagcatcgag gccttggaga agacgatgaa gccgtaggga tgcaggtgca
     3421 ggctgacccc gccggccagg gcctggctga tgtcgccgct ggcgatggcg cggcaggcct
     3481 ggtgaaaggc caccatggcc gacgagcagg ccgtgtccac ggccatgctg gggccgcgca
     3541 ggtcgaacac ataagagagc cggttggccg cgatgctggc ggtgttgccc gtggcggtgg
     3601 aggagtccat cgcggccatg tcgtcggcca ggcggtagga gtagtcggcg ctggcgatgc
     3661 ctatatagac gccgcagtcg ctgccgcgca gggcggaggg cttgatgccg gcgctttcga
     3721 tcgtttccca gcccagttcc agcagcaggc gctgctgcgg atccatgagg ctggcttcgc
     3781 gcggcgagat gccgaagaag tcggcgtcga agccggatat atcgccgatg gagccggcgg
     3841 cgaaggtata ggcggtgccg gggtgttcct tgccgggatg caggtaggca tcgtgcgacc
     3901 atctgccggc ctcgacctgg gtgaccaggt ctttgccctc gagcagatca ttccagtacc
     3961 gctgggtatc cgtgccggga aatctgaacg acattccgac gacggcaaca cgtttgacca
     4021 tgagcaactt tcttccggga tatgacaaca atcttgccgg gtttccgctg tgcagcaacc
     4081 gcgatgccag tgtgcctaga gtttggcggc gacccggggg aaatatgcaa caaaagaaaa
     4141 tagttttggg gggtaagcgc cgattaacgc caaaactgcg atttttattt tgggcgctgg
     4201 gcttggggca tgaacttcat gatttttctg gaaatggcaa tcaaatttga tgatagatga
     4261 tcgcaaattt gtgagtaaaa attatccgtc aaataagcat gctttgccat tgcaaggggt
     4321 gttcccaggc ttgcgacctt tcgaggcggg cgctccggtt ttggtaattt ggaagaaaaa
     4381 gcagggtaag tgcctgtgcc gtatggcttt caaggatgaa agccataaaa aaccaatcca
     4441 cgcgagggat tcatttctaa tccgaggtaa tacttgtttg gcaaagtatg taactcgttt
     4501 tgttaccatt tgggcggcaa gcgcaggcac agttagcaca gcggcgcatc ctgtcgccgt
     4561 atcggtttgg gaaatcccgc gccaactaaa gatatagggg tcaatcgcgc catttcagag
     4621 gtgccggcca ggcggcagcc ggcttgggtt attgcgcgag actttcatat ttcttccaac
     4681 aaaaatttcc ttttattaca tttgtatggg aaaccgggaa ctggcgtgta ctaaggttct
     4741 acggtgccgg caggacgcga aattggcata ttgctttggt aaaaaacctg aattttcaat
     4801 ttaaagaaaa ccagaaatga ccctacttgt gaccggcgga gccgggttta ttggcgcgaa
     4861 tttcgtattg gattggttta atcaatcagg agaaaccctg attaatttgg ataagctgac
     4921 ttacgccggc aatcccgaca cgctggcctc attgcagggc gatgcgcggc atgttttggt
     4981 gcagggcgac ataggcgaca gccaactggt ggccggcctg ctggccaagc accagccgcg
     5041 cgccgtggtc aactttgccg ccgagtcgca tgtcgaccgt tccatccacg ggccggccga
     5101 attcatgcag accaatgtca tgggcacctt caacctgctg gagtcggtgc gggcctacct
     5161 gccgaccttg cagcccgagg cccggcaggc cttccgtttc ctgcacgtgt ccaccgatga
     5221 ggtctacggc acgctcgcgc cgcaggaccc gccctttacc gaagaaaacc agtacgcgcc
     5281 caacagcccg tattcggcct ccaaggcggc gtccgaccac ctggtgcggg cctggcacca
     5341 tacctacggc ctgccggtgc tcaccaccaa ttgctccaac aactacgggc cttaccattt
     5401 tcccgagaag ctgattccgc tcatcatcca caatgccttg gcgggcaagc ccctgccggt
     5461 gtatggcgac ggcatgcagg tgcgcgactg gctctacgtg aaagatcact gcagcgccat
     5521 ccgccgcgtg ctcgaggccg gcgcgctggg cgagacctat aacgtgggcg gctggaacga
     5581 gcgcgccaac atcgatgtgg tgcgcaccat ctgcggcctg ctcgacgaac tgcggccgct
     5641 ggaggcgggg cgccattaca gcgacctgat cacctacgtc aaggatcgcc ccggccatga
     5701 ccgccgctat gccatcgacg cgcaccgcat cgagcgcgag ctgggctggc gcccggccga
     5761 gaccttcgag acgggcatcc gcaagacggt gcagtggtac ctggacaacc aggcctgggt
     5821 gcagggcgtg accagcggcg cctatcgcaa ctgggtatcc cgccagtacg gcacctgagg
     5881 aagtcttgcc atgaagattc tgatcactgg ctgcaaaggc caggtggggt tcgagctgca
     5941 gcgcagcctg gctgtgctgg gcgacgtggt gccggtggat cgcagccgct gcgacctggc
     6001 cgacccgcag agcatatgcg aggtgatgcg cgccgagcgt ccggacatcg tggtcaatgc
     6061 cgcggcctat acggcggtgg acaaggccga gcaggagcag gacctggccg cgcaggtcaa
     6121 cggccggggc ccgggcgtgc tggccgcgga ggccgagtcg ctgggcgcgc tgctggtgca
     6181 ttactccacc gactatgttt tcgacggcct gcagcacacg ccttacagcg agcgcgatgt
     6241 caccagcccg gctggcgcct atgggcgcac caagctggaa ggcgagcacg ccgtgcgcac
     6301 ggcgaccaag cgatacctga tcctgcgcac cagctgggtg tatggggtgc atggcggcaa
     6361 cttcctgaag accatgctgc gggtgatgcg cgagcgcgat gccttgcgcg tggtgggcga
     6421 ccaggtgggc gcgcccaccg gcgcggccct gatcgccgac gtcacggcgc acttgctgtc
     6481 gaagtacctg gccgcgcggg gcgagccgtt cccctacggg acttaccacc tggccgccaa
     6541 gggcgagacc acctggtacg actatgccgg cctgatcggc cgccaggcca tcgaggccgg
     6601 cgtgccgctg aaggtgcagc ccgagcagat ccagtgcatc accaccgagg actacccctt
     6661 gccggcccgc cgcccggcgt tctcgcggct ggataccacc aagctgcgcg agaccttcgg
     6721 cctggtgctc ccgccctggg aagatggggt aaagcatgca ttgaaggtgc tgctgccagg
     6781 ggccgcctga tgccgcggca gggcgggtct cctggccagt ctgccccggc ttccatcacg
     6841 cagggcgtct ggcgagtgat ttcacccacg ggcgtcgtca tggccgaggc gctgcgtttt
     6901 tttccggatg gacgcgtcgg cgggcatttg atggccgaca tgctgtcctg gcgcctggag
     6961 gaagacggcc tgccttgttt cctcggaccg ggaggcgagc cggccatccg caccacgata
     7021 ttgccgggcg gcggcggatg gctgcaggcc agttcggtcc gtacgggcag cgaagtcttc
     7081 cgcctgaaac agcatgattt cggcgaccgc aagcgctggt cggccctgac gcgcaaccac
     7141 tacgccaacg agattgccac ttatggctgg gagattggcg accatagcta cggtgtgccc
     7201 caggttttcg aacgcatggc caaattgaag atcggcaagt atgtgtcgat cgccgagggc
     7261 gtgcgcatcg cgttgggcaa ccactgcatg gataacgcca ccacgtatcc ctttgccgcg
     7321 ttgagcctat ggtggccgtc ggcgcccact ggcgcagaag acgaccatgc cacccggggg
     7381 gatgtggtca tcggtcatga tgtctggatt gccgcggggg ttttcatcgc ctctggcgtc
     7441 accatcggca atggcgcggt gattggcgcg gcggcggtgg tcacccgcga tgttccccct
     7501 tattccgtgg tggcgggcaa ccctgccagg gtgacccgtt accgcttcga tgcccggatc
     7561 attgccgaat tgcaggcatt ggcctggtgg cattggccgg acgaggtcgt tgatcgtttc
     7621 ctgccgctga tgctccagcg cgacttgcag gccttcattg acgccgcccg ggcgcaaggg
     7681 ctgggcccca aagccgagtc ggcctgagtt tcaggccggg ccacgtcagg ccgggccgcg
     7741 tcaggccggg ccgcgtcagg ccgggctgct ttcagtccag cgtctgcgcg tttttcctgg
     7801 gcgcggcggc gaccgccgag cgcgaggcgg gcagggtggc catgagctgg taggcgccgc
     7861 ggccggtgcc gggggcgtat tcgagcgggt cttcgcagca catcaggccc agctggatca
     7921 tctgcacctg ccaggtggcg ggtgggcttt gcggcgaggc cttgccgccg tcgaggccgt
     7981 cgacccacca gccggcgcgg gcgccgaaca ggaagtccat ccaggccgcg ggctgcaggc
     8041 cgcgcagcag cagggtgccg ccgggcttga ggtgccggct ggcaaagccc agggcccgca
     8101 cggctggctg gctggcggcg taatcggcat ggaaaagaat caggtcggcc tgcggcaccg
     8161 tggcggcggc cgtgtcgctc ggcgagagat aggtgtagtc gcagcggttg gcctgcagcg
     8221 cctcgcgcag ttcggcgccg aagaccgggc cgaaggggcc gatctcgagt aggccgatgt
     8281 gctgctcggc cgtgcccatg aggcgggcct ggctgatgcg gtcgaccagc acatcggcca
     8341 cgccgtgggt ggccgtttcg ttgccgacgt ggcggtacag cgcggtggcg ctgaggtctt
     8401 gcggataggc ctcgtcgacc gggcggctgc cggtgaggat gtggttcagg tacaggtcga
     8461 tgcggccgca ggcgtgcacg atgggcaggt attcgggctg ctcgcgcagc aggttgctcc
     8521 aggtgtagtc caggctgtgc ctgtcgaact gcggacgggc gatgagccag ttgccctcgg
     8581 gctgggggcg ggccaggcgg gccagcagcc agccgaggaa aggcgcggtt tcgggcgagg
     8641 cctggcggca ggcgtccagc gtctcggcgc tgagcaggcc ctgcctggac agccggtaca
     8701 gcacatgccg ggcatggcgg tcgatcaggt tttccaggcg gggttcgagt tcgtggacgt
     8761 agcggcgatg ccggcgttcg gcctcgacgc tgcgcagcgt tgcttccagg aaggggtgca
     8821 gggcgtgggc ctgcaatgcg atgctcgcca tgttggtttc ccgtagcggt aggcaagtcc
     8881 agtgtggctg gactcgcctg caaccgcttc ggcaagatgc aacaaaagca aatcgctcgt
     8941 ggttttgcgg ccttcgcgga cggcggggca aatgatggcg aaactgccag ggcctgtcgt
     9001 caaagttgtg ccggatcggg ccggatgccc ggccaggcgc gggcacggcc ggccggtgcc
     9061 gggccggatg ggccggcgtt cgagccgggc cgcatatatg cgcttacaac ttggatggcc
     9121 cttggaaaga tttgcgcaca tttggcggcg cgctggacga cggccgcccg gcggcaaatc
     9181 agagcccggc cacgcgcttg ccggtgtcgg cctcgaacca gtgcaagggg tggcggccgt
     9241 cggggccgat ctcgaggcgg gcgcccggct gcacctgcaa ttcggtcagc tgccgggtcg
     9301 tgcagcgcac gacgatctgg gcgttgccgt agcggcagtg caccagttgc tcggagccca
     9361 gggtctcgat catctcgacc tcggccgcca ggccgcgggt gttgagcatc atgtattcgg
     9421 ggcgcaggcc catgacgacc ttgcggccgc gcacgcccgg cggcacgtcg gtgggcggga
     9481 tgtccagcgg caggctgcct tcggcggttt gcagggtgcc gtcgccggcc acctggaccg
     9541 ggatgaggtt catgggcggc gaaccgatga agccggccac gaagaccgag gcgggtttct
     9601 cgaaaacctc gatcggcgtg ccgatctgct cgggcacgcc caggttcatg acgaccatgc
     9661 ggtgcgccag cgtcatggcc tcgacctggt cgtgcgtgac gtacaggctg gtggtcttga
     9721 gccggcggtg cagcttgagg atctccagcc gcatggccac gcgcagcttg gcgtcgaggt
     9781 tggagagcgg ctcgtcgaag aggaacacct tgggctcgcg cacgatggcg cggcccatgg
     9841 ccacgcgctg gcgctggccg ccggacagcg cgcgcggacg gcgctcgagc agcttggaga
     9901 gttccaggat ctgggcggcg tcctcgacgc gcttcttgat gtcttccttg gacagcttgc
     9961 gcagcttcag gccgtaggcc atgttgtcga acacgctcat gtgcgggtag agcgcgtagt
    10021 tctggaacac catggcgatg tcgcgctcgg ccggctcgcg gtcgttgacg gtgacgccgt
    10081 cgatctggat ctcgccgctg gtgacggttt ccaggccggc gaccatgcgc atcagcgtgg
    10141 atttgccgca gccggacggg ccgacgatga cgatgaactc gccgtcggcg atttccatgt
    10201 cgatgccgtg gatcaccgcc acgttgccgg cgtaggtctt cttgacttgc ttgaggctca
    10261 gggtggccat ggatgtgcgc gagcgtgtag ggggttactt ttcggagtcg accaggccct
    10321 ggacgaacca tctctgcatg atgatcacca ccaggacggg cgggatcatg gcgagcatgg
    10381 cggtggccat ggtgaggttc cactcggtga cgctgtcgcc gccgctgatc atgcgccgga
    10441 tgcccaccac gatggggtac atgtcttcct tggtggtgac gagcagcggc cagaggtatt
    10501 ggttccagcc gtagatgaac tggatgacga agagcgccgc gatgctggtc ttggacaagg
    10561 gcagcagcac gtcgcggaaa aagcggatgg gcccggcgcc gtcgatgcgc gcggcctcga
    10621 ccagctcgtc ggggatggtg aggaagaact ggcggaacag gaaggtggcg gtggccgagg
    10681 cgatgagcgg cagggccagg ccggcatagc tgttgagcag gcccaggtcg gaggcgacct
    10741 tgtaggtggg cgcgatgcgc acctcgacgg gcagcatcag cgtgatgaag atcatccaga
    10801 agaacagctt gcgtccggga aagcgaaagt acaccaccgc ataggccgag atgatggaga
    10861 tgaggatctt gccgatggcg atggaaagcg ccatgaccag gctcacccac agcatgcggc
    10921 ccacgtgcgt gcccgacgag cccgacgaga ggacgtgcag gtagttgtcg acgaggtgcg
    10981 agccgggcag cagcgacatg ggcgcgttgg cgacttcctg cgcggtctgc gtcgacgcga
    11041 tgaaggtcac gtacagcgga aaggccacga tggccaggcc ggcgagcagg atggcatggg
    11101 ccaggaaatc gagccagggg cggcgttcta ccatggttct cgtctttgaa tggtcagtac
    11161 tggaccttgc ggtcgatgta gcggaactgc gcgatggtca gcagcacgac gatgaccatc
    11221 agcaccaccg actgcgccgc cgaggagccc aggtccagcc cgt
//
LOCUS       DASWPO010000288        10240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_67468, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000288 DASWPO010000000
VERSION     DASWPO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67468"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGC69_15120"
     CDS             <1..572
                     /locus_tag="VGC69_15120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0296659.1"
                     /translation="GMLGTKRRTFGNIVGHAKGVSDLSSLSSWTAEQFDPTLSWSDVE
                     WIKRQWGGKLILKGIMDAEDARLAADSGADALIVSNHGGRQLDGAMSSVTALPSIVEA
                     VKGSAIEVWFDSGIRSGQDVLKAVALGARGVMIGRAFLYGLGAHGYAGVMRALEILYK
                     EMDVTMALCGRRSIASGDKSILVPGTYPE"
     gene            complement(622..939)
                     /locus_tag="VGC69_15125"
     CDS             complement(622..939)
                     /locus_tag="VGC69_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296660.1"
                     /translation="MHSDITVVFNDNRSIGLSADVDPSPQRAADARDWFDAAWTTLGC
                     EPLRPSGKVLLLDKILGVADALGYDTLTSDAKESREFTEHTALALNKPSIRVDIPVLT
                     VGY"
     gene            complement(971..1291)
                     /locus_tag="VGC69_15130"
     CDS             complement(971..1291)
                     /locus_tag="VGC69_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEY0296661.1"
                     /translation="MLITFHSKATANVLMRAEDAAPLLRAAGKTIGSDFPTHGVFTPD
                     QLVAAIAGLEAAIHAAPPPLDDDSDPRHTKVTLRQRAFPLLEMMHKALAADAAITWES
                     SRGW"
     gene            1410..3095
                     /gene="argS"
                     /locus_tag="VGC69_15135"
     CDS             1410..3095
                     /gene="argS"
                     /locus_tag="VGC69_15135"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0296662.1"
                     /translation="MLPEQQQQLISLIQAAVARILPDAQPNIQLERPKVAAHGDVATN
                     VAMQLAKPARRNPRELAQELVDTLMAETPARALIEAAEIAGPGFINLRLTAAARQAVI
                     ASVARQGEAYGRAASNGQKVLVEFVSANPTGPLHVGHARQAALGDAICSLYAASGWDV
                     TREFYYNDAGNQIHNLAISVQARGRGIDPDSPEWPADGYKGDYIADIANDFKARKTVQ
                     ASDGQPVAASGDLDNLDDILLFAVAYLRREQDLDLQAFGLAFDNYYLESSLYTSGRVE
                     QTVETLIAKGHTYEQDGALWLRTTELGTGDDKDRVMRKSEGGYTYFVPDVAYHKAKWE
                     RGFHHAVNIQGSDHHGTVARVRAGLQGLEEGIPKDFPAYVLHKMVKVMRGGVEVKISK
                     RAGSYVTLRDLIDWVGRDAVRYFLIQRRADTEFVFDIDLALSKSEENPVYYIQYAHAR
                     ICTMIANSGAQAAEIAAADAALLCAPSEFALMQRLAEFPAMVALAAGELAPHHVAFWL
                     RDCAADLHAWYSACRVLVDDTALKLARLRLADTTRQVLANGLALLGVSAPQRM"
     gene            3131..3823
                     /locus_tag="VGC69_15140"
     CDS             3131..3823
                     /locus_tag="VGC69_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0296663.1"
                     /translation="MASQRKASRSQAGSTMYGVLAGLLIGLAIAAVVAFYVTRAPMPF
                     VDKATRQADQGKMPDLSQADPNQGLSGGTVTVPPAPGPGGGNEPKGKMDDLGALIATL
                     PSNDKKPADAAIQPVPVPAAPPAPAPASKPAVVASAAPKAAPAAPAAAASTANGAYFL
                     QAGAYKQASDAESVRARVLLLGLQASIQRAEVNGAQWNRVRVGPFAKLDDMNRARIRL
                     ADNKIDSTVVRQ"
     gene            3920..4561
                     /locus_tag="VGC69_15145"
     CDS             3920..4561
                     /locus_tag="VGC69_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY0296664.1"
                     /translation="MQVRSITRRHFTVAALALAGLCFTSAGQAQGTPQPYRDVSPAQP
                     SDSPGKIEVLEFFAYTCPHCAAMEPMVAEWLKTAPQDVAFKQVPIAWNASMKPLQQLY
                     YALQAVGRLDLSPKVFNAIHVEHQRIFTKDAIVDWAVKQGVDRDKFLAVFDSFAVQSQ
                     VQRADQLSQAYNVDGTPSYTVGGKYLTSPVLAGNTYEGALQEVDKLIPMARGN"
     gene            complement(4740..5945)
                     /gene="metC"
                     /locus_tag="VGC69_15150"
     CDS             complement(4740..5945)
                     /gene="metC"
                     /locus_tag="VGC69_15150"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY0296665.1"
                     /translation="MSTSSKHIDTVLAHSGAAPFNPETGVAPVNLPPMRASTVRFRDI
                     AKLEEAQQRRAAGGRPASYGIYGMDTHIALEAIFKELEGGAHCALQPSGLASIAMVFL
                     GLLSAGDHALVADCVYGPVHEFNNAVLSRMGIEVTFFPSGATSAELEQLVKPNTRLLY
                     VETPGSLLFQMMDIPALAGFAQRHGLVLATDNTWGSGYIYRPLQLGAQVSVVAGTKYI
                     AGHSDVMLGAVVANDHAIFQRIARTQYAIGNSISADDIWLALRGVRTMPVRMAQHARN
                     AMTICEFLAARPEVSRLYNPAWPQDPGHALWRRDCTGANGMLAISLKFSPAQARRFVE
                     SLTLFGIGFSWGGFESLVQLVTPAELARHAYWSEGDLALVRLHIGLEDTRDVIADLTQ
                     ALESAAAVV"
     gene            complement(6016..6708)
                     /locus_tag="VGC69_15155"
     CDS             complement(6016..6708)
                     /locus_tag="VGC69_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY0296666.1"
                     /translation="MQDPADRDDAQLEHWLLTRYSCRAFLPEPVSRRVIERILELAQR
                     SASWCNCQPWQLAIASGAGVQRLRAALMARHAQGQEGQRDYAGPSAYEGVYLERRRES
                     GYQLYESLGIARGDRAAYQRQAARNFALFDAPHLAVITCDAALGAYGAIDCGGYVSTF
                     MLAARANGVASIAQAALAQYPEVLRRELGLAETQRVVCGISFGYEDGAHPANQYRTRR
                     AALADVVNWVDE"
     gene            complement(6717..7778)
                     /locus_tag="VGC69_15160"
     CDS             complement(6717..7778)
                     /locus_tag="VGC69_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0296667.1"
                     /translation="MNPAESRLQYPWGDAQPEPGAARQVAEGAAWVQMPLPFALNHIN
                     LWLLRDEIDGRQGWTIVDCGVALDETRTLWERVFETSLDGLPVLRVLVTHMHPDHVGL
                     AHWLCERWQAPLWMSMTDFMTASLWSSGRAAGGGAGGEQAVAHFARNGLTDLDAQEHI
                     RQRSNYYPGLVPAMPSRYARLMHGDTVRIGQHDWRVIVGYGHAPEHVSLYCADLKLLI
                     SGDMVLPRISTNISVFDYEPGGNPLPLYLDSLDRYAGLPEDTLVLPSHGRPFIGLHER
                     IRQQHEHHAARLDEVLAACAQPQSASDILPVLFKRKLDHHQLTFAMGEALAHLHALYY
                     AGKIERRTGDDGIIRFATA"
     gene            complement(7892..8620)
                     /locus_tag="VGC69_15165"
     CDS             complement(7892..8620)
                     /locus_tag="VGC69_15165"
                     /inference="COORDINATES: protein motif:HMM:NF017820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0296668.1"
                     /translation="MNHRYLLRYNITNKFQAVRDARSFVKIQKLLRLSINRALLIEKK
                     IPGERKPPYMPFADDLRRIVESNEAFMEVLRAVRSVSPPSWCVGAGAVRSLVWDVLHG
                     RQGWRVPADIDVAYFDGGDLSEESEAGYATALRALRPDLPWEVVNQARVHLWYGQRFG
                     QTVPALHTLEEALATWPEYATCVGVRLNEDDGLEIIAPFGLSDLFSMTLRHNPSRVGA
                     AEYRARQQAKRYPERWPRVTVVLP"
     gene            8670..9785
                     /locus_tag="VGC69_15170"
     CDS             8670..9785
                     /locus_tag="VGC69_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0296669.1"
                     /translation="MDTAAELIPVTLLTGFLGSGKTTVLNRLVQQPELADALIIINEF
                     GDMALDHMLVAHSTENLVMEMSSGCLCCTIRGDLVQTLRDIVWRFSRNGQRQFRRVVI
                     ETTGLADPLPIIHTLMTHPQIVDRYRLDGIVTTLDFATGGHTLDHHPEAVRQAAIADC
                     LLLTKADLVPSDAREALQRRLDGINPAAPRWEVSHGEVGAARVLNLGLFSTDGKSPDV
                     ERWLQAEAYADEAEHGHAHAAHEGHDHAAAHDHGEGPHHDVNRHDDHIQAFCFSVDEP
                     IPKDVAAAWLEALMGLVGSKILRFKGILNIEGNDTPVVLHGVQHILHPLATLPGWPGD
                     DRRSRLVFITQDVGREVIEDTFRVFSHVLPLAWEAER"
     gene            9934..10080
                     /gene="ykgO"
                     /locus_tag="VGC69_15175"
     CDS             9934..10080
                     /gene="ykgO"
                     /locus_tag="VGC69_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEY0296670.1"
                     /translation="MKVLSSLKDAKTRHRDCQVVRRRGRTYVICKSNPRFKARQGGAR
                     SKKS"
     gene            complement(10011..>10240)
                     /locus_tag="VGC69_15180"
     CDS             complement(10011..>10240)
                     /locus_tag="VGC69_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296671.1"
                     /translation="GVMKRVCAGQRRNDSPMRPASSTPNEPRHLLQRQGHADASRAGP
                     DDGDIQAVHQDFLLLAPPWRALKRGFDLQMT"
BASE COUNT         1721 a         3460 c         3396 g         1663 t
ORIGIN      
        1 tgggcatgct ggggaccaag cgacggacct ttggcaacat cgtcggccat gccaagggcg
       61 taagcgacct gtcctcattg tcgtcgtgga cggccgagca gttcgacccg accttgtcct
      121 ggagcgacgt ggaatggatc aaaaggcagt ggggcggaaa gctcatcctc aagggcatta
      181 tggatgccga ggatgcacgc ctggccgccg acagcggcgc cgacgcgctc atcgtcagca
      241 accatggcgg gcgccagctc gatggcgcga tgtcgagtgt cacggcgctg ccttccatcg
      301 tcgaggcagt gaaaggctcc gccatcgagg tctggttcga tagcggcatc cgctcgggcc
      361 aggacgtgct caaggcggtg gccttgggcg cacgtggcgt gatgataggc cgcgccttcc
      421 tgtacggctt gggcgctcat ggctatgccg gagtgatgcg cgcgctggaa attctctaca
      481 aggaaatgga tgtgaccatg gcgctatgtg ggcgcaggag catcgccagc ggcgacaaga
      541 gcatcctggt gccgggtacc tatccggaat gacgggcgtg gtcaccgctg ccgccacgcc
      601 gcctgcgcgt gctcgcggcg tctagtaacc caccgtcaac acggggatat ccaccctgat
      661 gctcggcttg ttcagggcca gggcggtgtg ttcggtgaac tcgcggcttt ccttggcgtc
      721 gctggtgagg gtgtcgtagc ccagcgcgtc ggccacgccc aatatcttgt cgagcagcag
      781 caccttgccg ctggggcgca agggttcgca gcccagggtg gtccaggcgg cgtcgaacca
      841 gtcgcgggcg tcggcggcgc gttgcgggga cgggtcgacg tcggcgctca ggccgatgct
      901 gcggttgtcg ttgaagacga cggtgatgtc gctgtgcatg ggcaggctcc tttgatcgtg
      961 cggtggggcg ctaccagccg cgcgagcttt cccaggtgat ggcggcgtcg gcggccagcg
     1021 ccttgtgcat catctcgagc aggggaaaag cgcgctggcg cagcgtgacc ttggtgtggc
     1081 gcggatcgga gtcgtcgtcc aggggcggcg gcgcggcgtg gatggcggcc tccaggccgg
     1141 cgatggcggc gaccagctgg tcgggcgtga acacgccgtg ggtggggaaa tcgctgccga
     1201 tggttttgcc ggcggcccgc agcagcggtg cggcgtcttc ggcgcgcatc aggacgttgg
     1261 ccgtcgcctt ggaatgaaat gtgatcagca taaagccttt ctccaggagg atttaacgtt
     1321 acgctactcc aagcgggccc gcttagctag ccgggaggcg cgggcaattc acgttatgat
     1381 tcagggtatt acctctcgcg atttacctca tgctccccga gcaacagcaa caactcatct
     1441 ccctgatcca ggccgccgtc gcgcgcattc tccccgacgc gcagcccaat atccagctcg
     1501 agcgccccaa agtcgccgcg cacggcgacg tcgccaccaa tgtcgccatg cagctggcca
     1561 agccggccag gcgcaatccg cgcgagctgg cccaggagct ggtcgacacc ctcatggccg
     1621 aaaccccggc gcgcgccctg atcgaggccg ccgagatcgc cggccccggt ttcatcaacc
     1681 tgcgcctgac cgccgcggcc cgccaggcgg tgatcgcctc ggtggcgcgg cagggcgagg
     1741 cctacggccg cgccgcgtcc aacggccaga aggtgctggt ggagttcgtc tcggccaacc
     1801 ccacgggccc gctgcacgtg ggccatgccc gccaggccgc gctgggtgac gccatctgca
     1861 gcctgtatgc cgccagcggc tgggacgtga cccgcgagtt ctactacaac gacgccggca
     1921 accagatcca caacctggcc atcagcgtgc aggcccgcgg gcgcggcatc gatccggatt
     1981 cgcccgagtg gccggccgac ggctacaagg gcgactacat cgccgacatc gccaacgatt
     2041 tcaaggcgag gaagaccgtg caggcctccg acggccagcc cgtggcggcc agcggcgacc
     2101 tcgacaacct ggacgacatc ctcctgttcg ccgtggccta cctgcgccgc gagcaggacc
     2161 tggacctgca ggccttcggc ctggccttcg acaattacta cctcgaaagc tcgctctaca
     2221 cctcgggccg cgtcgaacag accgttgaaa cgctcattgc caagggccac acctacgagc
     2281 aggacggcgc gctgtggctg cgcaccaccg agctcggcac cggcgacgac aaagaccgcg
     2341 tgatgcgcaa aagcgagggc ggctacacct atttcgtgcc cgacgtggcc taccacaagg
     2401 ccaagtggga gcgcggcttc caccacgccg tcaacatcca gggcagcgac caccacggca
     2461 ccgtggcgcg cgtgcgcgcc ggcctgcagg gcctggaaga gggcatcccc aaggatttcc
     2521 ccgcctacgt gctgcacaag atggtcaagg tgatgcgcgg tggcgtcgag gtgaagatct
     2581 ccaagcgcgc cggcagctac gtcaccctgc gcgacctgat cgactgggtg ggccgcgacg
     2641 cggtgcgcta cttcctcatc cagcgccgcg ccgacaccga gttcgtgttc gacatcgacc
     2701 tggcgctgtc caagagcgaa gaaaaccccg tctactacat ccagtacgcc cacgcgcgca
     2761 tctgcaccat gatcgccaac tcgggggcgc aggccgccga gatcgccgcg gccgacgccg
     2821 cgctgctctg cgcgcccagc gagttcgcgc tgatgcagcg cctggccgag ttcccggcca
     2881 tggtggccct ggccgccggc gagctggccc cgcaccacgt ggccttctgg ctgcgcgact
     2941 gcgcggccga cctgcacgcc tggtattctg cctgccgcgt gctggtcgac gacacggcgc
     3001 tcaagctcgc ccgcctgcgc ctggccgaca ccacgcggca ggtgctggcc aacggcctgg
     3061 cgcttctggg cgtctcggcg ccgcaacgca tgtaatccgc agggcatcgc tgcatctgga
     3121 ccccaagact atggcctcgc aacgcaaagc ttcacgttcc caggcgggca gcacgatgta
     3181 cggcgtgctc gccggcctgc tgatcggcct ggccattgcc gccgtcgtcg ccttctatgt
     3241 aacgcgcgcg cccatgccct tcgtcgacaa ggccacccgc caggccgatc agggcaagat
     3301 gcccgacctg agccaggccg accccaatca gggcctgtcc ggcggcacgg tcacggtgcc
     3361 gcccgccccc ggaccagggg gcgggaacga acccaagggc aagatggacg acctgggcgc
     3421 attgatcgcc accctgccca gcaacgacaa aaagcccgcc gacgcggcga tccagcccgt
     3481 ccccgtgccg gccgcgccgc ccgctcctgc ccccgcgtcc aagccggccg tcgtggcctc
     3541 ggcggcgccc aaggccgccc cggcagcccc ggctgccgcc gccagcaccg ccaacggcgc
     3601 gtatttcctg caagccggcg cctataagca ggcttccgac gcggaatcgg tgcgcgcccg
     3661 cgtcctgctg ctgggcctgc aggcctcgat acagcgcgcc gaggtcaacg gcgcgcagtg
     3721 gaaccgggtg cgcgtgggcc ccttcgccaa gctggacgac atgaaccgcg cccgcatccg
     3781 gctggccgac aacaagatcg actcgacggt agtacgccaa taatcatcgt gcccgattga
     3841 acttccgggc gggtgtcccg gtcggataat cgggctcgcc aactccccct tttcaggaag
     3901 acccttcatc atgccccgta tgcaggttcg ttcaatcacc cggcgccatt tcaccgtcgc
     3961 cgcactggcc ctcgcgggcc tttgcttcac ctcggccggc caggcccagg gcaccccgca
     4021 gccctaccgg gacgtctcgc ccgcccagcc gtccgactcg ccgggcaaga tcgaggtgct
     4081 cgaattcttc gcctatacct gtccgcactg cgccgccatg gagcccatgg tcgccgaatg
     4141 gctcaagacc gcgccccagg acgtggcctt caagcaggtg cccatcgcct ggaacgccag
     4201 catgaagccg ctgcagcagc tgtactacgc gctgcaggcc gtgggccgcc tcgacctcag
     4261 ccccaaggtg ttcaacgcca tccacgtcga gcaccagcgc atcttcacca aggacgccat
     4321 cgtcgactgg gccgtcaagc agggcgtgga ccgcgacaag ttcctggccg tgttcgattc
     4381 cttcgccgtg cagagccagg tgcagcgcgc cgaccagctg tcccaggcct ataacgtcga
     4441 cggtacgccc agctacaccg tgggcggcaa gtacctgacc tcgccggtgc tggcgggcaa
     4501 cacctacgaa ggcgccctgc aggaagtcga caagctgatc ccgatggcgc gcggcaacta
     4561 atcttgccaa gccaggcaaa aagaccgctg atgcggtctt ttttattgtt gcgaaccaag
     4621 acgatgtgag ccgaggaccc cggaggaatc cgaagcacag ggggcgtctt agcccccgag
     4681 catcggaatt ccgcagggca gcctcggcgg ctcaagcacc caaaagcgct taaaagcact
     4741 caaacaacgg ccgccgcact ctccagggcc tgcgtcagat ccgcgatcac atcccgcgta
     4801 tcctccagcc cgatatgcag ccgcaccagc gccaggtcgc cctccgacca atacgcatgc
     4861 cgcgccagct cggccggcgt caccagctgc accaggctct cgaagccgcc ccatgaaaat
     4921 ccgatgccga acagcgtgag cgattccacg aaacgccgtg cctgcgccgg cgagaacttg
     4981 agcgaaatcg ccagcatgcc gttggcgccc gtgcagtcgc gccgccacag cgcgtggccc
     5041 gggtcctgcg gccaggctgg gttgtagagg cgcgagacct cgggccgcgc ggccaggaac
     5101 tcgcagatcg tcatggcgtt cctggcgtgc tgggccatgc gcacgggcat ggtgcgcacg
     5161 ccgcgcaggg ccagccagat gtcgtcggcg ctgatcgaat tgccgatggc gtattgcgtg
     5221 cgggcgatgc gctggaagat cgcgtggtcg ttggccacca ccgcgcccag catcacgtcg
     5281 gaatggcccg cgatgtactt ggtgcccgcc accacggata cctgcgcgcc cagttgcagc
     5341 gggcgataga tatagcccga gccccaggta ttgtcggtgg ccagcaccag gccgtggcgc
     5401 tgggcgaacc cggccagggc ggggatgtcc atcatctgga agagcagcga gccgggcgtc
     5461 tccacgtaca agaggcgcgt attgggcttg accaactgct ccagctcggc cgaggtcgcg
     5521 ccgctgggga agaaggtgac ctcgatgccc atgcgcgaca gcacggcgtt gttgaactcg
     5581 tgcacggggc cgtacacgca atcggccacc agcgcgtggt cgcccgccga cagcaggccc
     5641 aggaagacca tggcgatgga agccaggccc gagggctgca gggcgcaatg cgcgccgcct
     5701 tccagttcct tgaagatcgc ctcgagcgcg atgtgcgtgt ccatgccgta gatgccgtag
     5761 ctggcgggac ggccgcccgc ggccctgcgc tgctgcgctt cttccagctt ggcgatatcg
     5821 cgaaagcgca cggtgctggc gcgcatcggc ggcaggttga cgggggcgac gccggtctcg
     5881 ggattgaacg gggcggcgcc ggagtgcgcc agcaccgtgt cgatgtgctt ggaagaggtg
     5941 gacatggcgg gtcggtgaaa tgatgggcag tccaatctta ccctcgcctg ccggcccgca
     6001 tggacgctgc gtgccctatt cgtcgaccca gttcaccacg tcggccaggg ccgcgcgccg
     6061 ggtccggtat tgattggccg gatgggcgcc atcttcataa ccaaaagaaa taccgcacac
     6121 gacccgttgc gtctcggcaa gcccgagttc gcggcgcagc acctcggggt attgcgcgag
     6181 cgcggcctgc gcaatgctgg ccacgccgtt ggcgcgcgcg gccagcatga aggtggacac
     6241 atagccgccg caatcgatgg cgccgtatgc gccgagcgcg gcatcgcagg tgatgacggc
     6301 caggtgcggc gcatcgaaca gggcgaagtt gcgcgccgcc tggcgctggt aggccgcgcg
     6361 gtcgccgcgc gcgatgccca gcgactcgta cagctggtag cccgattcgc ggcggcgttc
     6421 caggtatacg ccttcgtagg cgctgggccc ggcgtagtcg cgctgccctt cctggccttg
     6481 cgcgtggcgg gccatgagcg ccgcgcgcag gcgctgcacc cctgcgcccg atgcgatggc
     6541 cagttgccag ggctggcagt tgcaccacga cgcgctgcgc tgcgccagtt cgagtatgcg
     6601 ttcaatgacg cggcgcgaca ccggctcggg cagaaaagcg cggcagctgt agcgggttag
     6661 cagccagtgc tccagctgcg cgtcatcgcg gtcggcgggg tcttgcatgt cgggctttat
     6721 gccgtcgcga agcggatgat gccgtcgtcg ccggtgcgcc gctcgatctt gcccgcgtag
     6781 tacagcgcgt gcaggtgcgc cagggcctcg cccatggcga aggtcagttg atgatggtcg
     6841 agcttgcgct tgaacagcac cggcaggatg tcggacgcgg attgcggctg cgcgcaggcg
     6901 gccagcacct cgtccaggcg ggccgcgtgg tgttcgtgct gctgcctgat gcgctcgtgc
     6961 agcccgatga agggccggcc gtgcgagggc agcaccagcg tgtcctcggg caggccggca
     7021 tagcggtcga gcgaatccag gtagagcggc agcgggttgc cgccgggctc gtagtcgaac
     7081 acgctgatgt tggtcgagat gcgcggcagc accatgtcgc ccgagatcag cagcttgaga
     7141 tccgcgcagt acagcgacac gtgttcgggc gcgtggccgt agcccacgat gacgcgccag
     7201 tcgtgctggc cgatgcgcac ggtgtcgccg tgcatcaggc gcgcatagcg cgacggcatg
     7261 gccggaacca ggccggggta gtagttggac cgctggcgga tgtgctcctg ggcgtcgagg
     7321 tcggtcaggc cgttgcgggc gaagtgggcc acggcctgct cgccgcccgc gccgccgccc
     7381 gccgcgcggc ccgaggacca cagcgaggcc gtcatgaagt cggtcatgct catccacagc
     7441 ggcgcctgcc agcgctcgca cagccagtgg gccaggccga cgtggtcggg gtgcatgtgg
     7501 gtgaccagca cgcgcagcac cggcaggccg tcgagcgagg tctcgaagac gcgctcccat
     7561 agcgtgcggg tctcgtcgag cgccacgccg cagtcgacga tggtccagcc ctggcggccg
     7621 tcgatctcgt cgcgcagcag ccagaggttg atgtggttca gggcgaaggg cagcggcatt
     7681 tgcacccagg ccgcgccctc ggccacctgg cgggccgcgc cgggctcggg ttgggcgtcg
     7741 ccccaggggt actgcagtcg gctttcagcg ggattcatgc tgtctctctc ctctcgttct
     7801 ctacagcata cgacaaacgc cgctgccgcc cggcttcccg tcattgagcg gcaacgcggc
     7861 gccaaagcga tgcggccggt tcagggcgcg cttacgggag caccaccgtg acccggggcc
     7921 aacgctcggg gtatcgcttg gcttgctgcc tggcgcggta ttcggcggcg ccgacgcggc
     7981 tcgggttgtg ccggagggtc atcgagaaca ggtcgctcag gccgaaaggc gcgatgatct
     8041 ccagtccgtc gtcttcgttc agccggacgc ctacgcaggt cgcgtattcg ggccaggtcg
     8101 cgagcgcttc ttccagcgta tgcagcgcgg gcaccgtctg gccgaaccgt tgcccgtacc
     8161 agagatggac ccgggcctgg ttgacgacct cccaaggcag gtcggggcgt agcgcccgca
     8221 gcgcggttgc atagccagcc tcgctttcct cggacaggtc gccgccgtcg aagtaggcca
     8281 cgtcgatgtc cgcgggaacc cgccagcctt gccggccgtg caggacatcc cagacgaggg
     8341 accgcaccgc gccggctccg acacaccagg agggagggga gacgctgcgc acggcccgca
     8401 gcacctccat gaaggcttcg ttgctctcta cgatacggcg caggtcatcg gcaaacggca
     8461 tgtacggcgg cttcctttcc ccaggaattt tcttttcgat caataaggcc ctgttgatcg
     8521 acaagcgcaa tagtttttgg attttgacaa agcttcgagc gtcgcgcact gcttggaatt
     8581 tatttgttat gttataacgt aacaaataac gatgattcat cggataacgc cgatgcaact
     8641 aacttctcac taagcgaggt cctttgagca tggataccgc cgccgagcta attcccgtca
     8701 cgctgctgac gggctttctt ggcagcggca agaccacggt cctcaaccgc ctggtgcagc
     8761 aacccgagtt ggccgatgcc ctgatcatca tcaacgaatt cggcgacatg gcgctggacc
     8821 acatgctggt ggcgcacagc accgagaatc tggtgatgga gatgagtagc ggctgcctgt
     8881 gctgcaccat ccgcggtgac ctggtgcaga cgctgcgcga tatcgtctgg cgtttttcgc
     8941 gcaacggtca gcgccagttt cgccgcgtgg tgatcgagac gaccggcctg gccgacccgt
     9001 tgcccatcat ccacacgctg atgacgcatc cgcagatcgt ggatcgctac cgactggacg
     9061 gcattgtcac gaccttggac tttgccactg gcgggcacac gctggaccat catcccgagg
     9121 ccgtccggca ggctgccatc gccgattgcc tactgcttac caaggccgat ctggtgccgt
     9181 cggacgcgcg cgaggcgttg cagcgccggc tcgatgggat caatccggcc gcgccgcgct
     9241 gggaggtcag ccacggtgag gtcggcgcgg cccgggtgct gaacctgggg ctgttctcga
     9301 ccgacggcaa gtcccccgac gtcgaacgtt ggctccaggc agaggcctac gcggacgagg
     9361 cagagcacgg gcacgctcat gcggcacacg aaggccacga tcatgccgca gcccacgatc
     9421 acggcgaggg cccccaccac gacgtcaacc gccatgacga ccatatccag gcgttctgct
     9481 tctcggtgga cgagcccatc ccgaaagacg ttgccgcggc ctggttggaa gcgctgatgg
     9541 gtctggtcgg cagcaagatc ttgcggttca agggcatctt gaacatcgaa ggcaacgata
     9601 cgcccgtcgt ccttcatggt gtgcagcata tccttcatcc gttggccacc ctgccaggtt
     9661 ggcccggtga cgaccggcgt tcgcgcctgg tgttcatcac ccaggatgtc ggccgggagg
     9721 tcatcgaaga cactttccgc gtgttcagcc acgtgctgcc cctggcatgg gaagccgagc
     9781 gatgaccctg gcgatccaac tctacaacat cgagcatccc aagcgggggg aatggctgac
     9841 gcgcgagctg tattcgaccc gcaccgacgc cgagatcgag accccatgaa acccgcctgt
     9901 tgattgttcc cgctttgctc gaaaggatct cccatgaaag ttctctcctc gctgaaggat
     9961 gccaaaaccc gccaccgcga ttgccaggtc gtgcgtcgcc gcggccgcac ctacgtcatc
    10021 tgcaagtcga acccgcgctt caaggcccgc cagggtggcg ccagaagtaa gaaatcctga
    10081 tgcaccgcct ggatgtcgcc atcgtcgggg ccggcgcggc tggcatcggc atggccctgg
    10141 cgctgcagaa ggtgccgggg ctcatttggc gtgctggagg ccggccgcat gggtgagtcg
    10201 ttccggcgct ggccggcgca gacgcgcttc attacgccgt
//
LOCUS       DASWPO010000289         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1227577, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000289 DASWPO010000000
VERSION     DASWPO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227577"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..355)
                     /locus_tag="VGC69_15185"
     CDS             complement(8..355)
                     /locus_tag="VGC69_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEY0296672.1"
                     /translation="MTEDRDVWLFSYGTLQLESVQLESFGRLLGGEDDAMIGYSQAMV
                     EITDPEVIRQSGQTFHPIVAPTGNTTDEVVGKVFRITREELAAADRYEVSDYQRVLVP
                     LKSGKQAWVYVRA"
     gene            complement(461..1360)
                     /locus_tag="VGC69_15190"
     CDS             complement(461..1360)
                     /locus_tag="VGC69_15190"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296673.1"
                     /translation="MSHTILVTGASSGFGLMTAKALALAGHTVYASMRETQGRNATRV
                     AEIAQWSTDNAADLRTVELDVQSDTSPTTAIARILQDAGRLDVIVHNAGHMVFGPAEA
                     FTPEQLMQQYDVNVLGTQRVNRAALPHLRKQGSGLLVWVGSSSTRGGTPPFLAPYFAA
                     KAAMDALAVSYSTELSRWGIETTIMVPGAFTQGTNHFAHSGKPADTAVAAEYENGLYA
                     DVPGKALEGLKNLEPEDADPAEVAREIVRVVGLPFGKRPFRVHVDPSQDGAEVVNAVA
                     DRMRREMYHAIGLKDLLSPAVKG"
     gene            complement(1412..2044)
                     /locus_tag="VGC69_15195"
     CDS             complement(1412..2044)
                     /locus_tag="VGC69_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEY0296674.1"
                     /translation="MKLLLLGATGLVGRNVLQQALASDKIGQVIAPTRRPLPAHPKLD
                     NPTASDLRQVAPQALRHPVDAVVCAMGTTIAKAGSKAAFRHVDYVLPILFAGMARQQG
                     ARSLALVSSPGASLSIPLFYCRIKGEVERDMRAMGFESLAIIRPGMIGGHRDEFRLAE
                     RLIFPVAAMLGPILPRGLRINPAANIARALLEAVIAQAPGERLMTSKDLV"
     gene            complement(2041..2595)
                     /locus_tag="VGC69_15200"
     CDS             complement(2041..2595)
                     /locus_tag="VGC69_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296675.1"
                     /translation="MPATRHSGPRPWRQRLRAVAVGLASVLALAGCGRERVPDAENNM
                     TMHPSVTYLHLLRHTPFFTSLSTEQLQWVIDHSREWEVDAGAVLSKRGPQGPSDTDYW
                     ILLDGGWRVDHDGKSFPSGHADPGKWFNTREAQGKPCALVATEHGYVMRIPEGEMQAM
                     LERQFAFDAHLEQGRAYYRQVFGK"
     gene            complement(2613..>2820)
                     /locus_tag="VGC69_15205"
     CDS             complement(2613..>2820)
                     /locus_tag="VGC69_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296676.1"
                     /translation="VNCVAPGPVATELFLNGKTEEQIQTFSKMPPLERLGQPEDIANV
                     VAFLAGPDAAWVNGQVLRANGGVA"
BASE COUNT          439 a          945 c          968 g          468 t
ORIGIN      
        1 ccttctttca cgcgcggacg taaacccagg cctgcttgcc ggacttgagc gggacaagca
       61 cgcgctggta gtccgagact tcgtagcggt cggccgcggc aagttcctcc cgggtgatac
      121 ggaaaacctt gccgacgact tcgtcggtcg tattgcccgt cggcgcgacg atgggatgga
      181 atgtctggcc gctttggcgg atgacctcgg ggtccgtgat ttccaccatg gcctggctgt
      241 agccgatcat ggcatcgtct tcgccgccca gcaggcgtcc gaaggattcg agctgcacgc
      301 tttcgagctg caatgtcccg taggagaaca gccatacgtc ccggtcttcc gtcatgtttc
      361 cctctactgg cctcggtgcc aatcttaccg cggccagcga tccgcatcgc cggcccgggc
      421 agtcgggcag ccccggccgg cgtcatatcg tcccgcctac ctagcccttg acggcgggcg
      481 agagcaggtc cttcaagccg atggcgtggt acatctcgcg gcgcatccgg tcggccacgg
      541 cgttgaccac ttccgcgccg tcctgcgacg gatcgacgtg cacgcggaag gggcgcttgc
      601 cgaacggcag ccccaccacc cggacgatct cgcgcgccac ttcggcggga tcggcgtcct
      661 cgggttccag gttcttcagg ccttcgagcg ccttgccggg cacgtcggcg tagaggccgt
      721 tctcgtattc ggcggccacg gccgtgtcgg ccggcttgcc cgaatgcgcg aagtggttgg
      781 tgccctgggt gaaggcgccc ggcaccatga tggtggtctc gatgccccag cgcgacagct
      841 cggtcgagta cgacacggcc agcgcgtcca tggcggcctt ggcggcgaag tacggggcca
      901 ggaacggcgg cgtgccgccg cgcgtcgacg acgaacccac ccacaccagc agcccgctgc
      961 cctgcttgcg caggtgcggc agggcggcgc ggttgacgcg ctgcgtgccc agcacgttga
     1021 cgtcgtactg ctgcatcagc tgctcgggcg tgaaggcctc ggccgggccg aacaccatgt
     1081 ggccggcgtt gtgcacgatc acgtccaggc ggccggcgtc ctgcaggatg cgggcgatgg
     1141 ccgtcgtggg cgacgtgtcc gactgcacgt cgagttcgac ggtgcgcaga tcggccgcgt
     1201 tgtccgtgga ccactgggcg atttccgcca cgcgcgtggc gttgcggccc tgggtctcgc
     1261 gcatcgaggc gtagacagta tggccggcca gcgccagcgc cttggccgtc atcaggccga
     1321 aacccgacga agcgccggtg acgaggatgg tatgtgacat gagggtctcc ctatggactg
     1381 aagaaaaatt caaggaactt cgataacgga ttcaaaccag gtccttcgac gtcatcaggc
     1441 gttcgccggg cgcctgggca atgaccgctt ccaacagcgc ccgggcgatg ttggcggcgg
     1501 ggttgatgcg cagcccccga ggcagaatcg ggcccagcat cgcggcaacc gggaaaatca
     1561 gccgctcggc gagccggaac tcgtcccggt gcccgcctat cattcccgga cggatgatgg
     1621 cgagcgactc gaaccccatc gcgcgcatgt ccctttcgac ttcccccttg atgcggcaat
     1681 agaacagggg aatggacagc gacgcgcccg gcgacgacac cagcgccaag gaccgcgctc
     1741 cctgctgccg ggccatcccg gcgaacagga tgggcagcac gtagtccacg tgccggaacg
     1801 cggccttgga tcccgccttg gcgatcgtcg tgcccatcgc gcagaccacg gcgtcgacgg
     1861 gatgccgcaa ggcctgcggc gcgacctggc gcaggtcgga agcggtcggg ttgtcgagct
     1921 tgggatgcgc cggcagcggg cgccgcgtgg gcgcgatcac ctggccgatc ttgtcgctcg
     1981 ccaaggcctg ctgcaggaca ttgcgtccca ccagccccgt ggcgcccagc agcaagagct
     2041 tcatttgccg aacacctgcc ggtagtacgc gcgcccctgt tcgagatgcg cgtcgaaggc
     2101 gaactggcgc tcgagcatgg cctgcatctc gccctcggga atgcgcatga cgtagccgtg
     2161 ctccgtggcg accagggcgc agggcttgcc ctgcgcttcg cgggtgttga accacttgcc
     2221 cgggtccgcg tgcccgctcg ggaacgattt gccgtcgtga tcgacccgcc acccgccgtc
     2281 gagcaggatc cagtagtcgg tgtccgacgg accttgcggg ccgcgcttgg acagcaccgc
     2341 gccggcatcg acctcccact cgcgcgagtg gtcgatgacc cactgcaact gttcagtcga
     2401 cagcgaggtg aaaaacggcg tgtgccgcaa caggtgcagg taggtaacgg atgggtgcat
     2461 ggtcatgttg ttctccgcat cggggacgcg ttcgcggccg cagcccgcga gcgccagcac
     2521 cgatgccaaa cccacggcga cagcgcgcag gcgctggcgc catggtctcg ggccgctgtg
     2581 tcgggtcgcc ggcatgggac gcgaggccgc gatcaggcca cgccgccgtt ggcgcgcagc
     2641 acttggccgt tgacccaggc agcgtcgggg ccggccagga aggcgaccac gttggcgatg
     2701 tcctcgggct ggcccaggcg ttccagcggg ggcatcttgg aaaaggtctg gatctgttcc
     2761 tcggtcttgc cgttgaggaa cagctcggtg gccacggggc ccggcgccac gcagttcacc
//
LOCUS       DASWPO010000290         6773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2836716, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000290 DASWPO010000000
VERSION     DASWPO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836716"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VGC69_15210"
     CDS             complement(<1..796)
                     /locus_tag="VGC69_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0296677.1"
                     /translation="MSSYHKWRTAAAVILCQAALGGASLASAQSVSMATWGGGVGDTW
                     RNAFAGPFQNESGTKVNITEVPNPEAQVRTQAKAPQYNAVLVGYFEAARLYRDGLLET
                     FSDSEIPQLADTPEQYKMRAADGKLIGAPVYFTYYGIAFNTDYVKPSEMQSWSDLGNP
                     KWKGQIAVTQPIYSSTYHLTILSHAMGGDEKDISASIPMFKKQVANASVVYTSLAQLN
                     QLLTRGEVVAAPYYLARIWAMKRDGVKNVDISLPKEGGLMLPYMVVV"
     gene            959..1597
                     /locus_tag="VGC69_15215"
     CDS             959..1597
                     /locus_tag="VGC69_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0296678.1"
                     /translation="MINPMLDDSPDDTQPEDGQPTKMDAVVGQRIRALRLARDLSLKE
                     VAVRSGLSIGLISQVERGISSPSLRVLGALSGALEVAIAYFFPEPAESQRDPKSPIIR
                     VNQRPALTFWRTGIAKSVLTPDVPGNNIDLVMYTIIIEPNGTTGDEPFTHDGEEAGVV
                     LEGTLDLHIGDQEYVLNEGDGFRFSSSIPHRFFNSTKRVARIMWVNCRERAV"
     gene            1727..2812
                     /locus_tag="VGC69_15220"
     CDS             1727..2812
                     /locus_tag="VGC69_15220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296679.1"
                     /translation="MSDPILTIDHVEKRYAGFHAVKGVSMVVERGEFLTLLGPSGCGK
                     TTLLRMIAGLEQPSSGEILIGGAPVTRMPPYKRNLGMVFQNLALFPHLTVGENVAFGL
                     KVRHTDTAAIAPAVTSALGLVGLDHLADRAVHQLSGGQRQRVALARALAVRPEVLLLD
                     EPLSALDMKLRRQLQVELKQLQRQVGTTFIFVTHDQEEALSMSDRIAVMNDGRVEQLA
                     SAQEVYTRPATLFAARFVGDNNVLTARAGDVGTDPGKLYFPALGMARPVPGLASLAPG
                     QEVHVAVRPEHIRVQTAETGTAVVENQVYSGSSVRLALRTATDTILATIAADATTVAS
                     YAVGTRVHIDWPDTACTVIPAAAGAPL"
     gene            2809..3642
                     /locus_tag="VGC69_15225"
     CDS             2809..3642
                     /locus_tag="VGC69_15225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296680.1"
                     /translation="MKLNRLALLLLLPAGLVMAVFVGGLVYLAWQSLVTPTGLGLQNY
                     ADFFQRHDYVLVLLRTLEIAVITTAVTLLISYPAAYAIARAGKRRNALLLLVILPWLV
                     SLVVRTYGWVVLLGNRGTLNSLLAWLGATDGPIRLLFNQTGVIIGLVHVFCPFMILSI
                     LTVFMHTERVLQEASAMLGANPLQTFLRVVGPLSLPGVISGCTIVFLLSTGAVITPLL
                     LGGPRNAMLATQIYQDVFQLFNFPKAAAMAFLLTLCAAILVWPLQVLEKRMMRHMKGA
                     Q"
     gene            3642..4442
                     /locus_tag="VGC69_15230"
     CDS             3642..4442
                     /locus_tag="VGC69_15230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296681.1"
                     /translation="MIAWLGRSAWRVLLVLIFAFLPAPIVVVVLSSFSPTGYLTFPPK
                     GISLRWYEEFLSSGDWLHALAISAVLAVAVAVVTTALGFMAGLATSRVKFPGRGAFGL
                     LMLSPLLFPHAAIAVALLGILSWRNAVGTYQGIFLAHAILCMPFAYRPLLNSMRKLDL
                     AMEEAAMMLGATPSQIFRLVTLPLLKPGLVTALLFSFIISFDEVTVTAFLLGPHVTTL
                     PTRIFSFVQESASPVIAAISTFLVIFTLLIVVLLDRLVGIELFVETER"
     gene            4551..5492
                     /locus_tag="VGC69_15235"
     CDS             4551..5492
                     /locus_tag="VGC69_15235"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296682.1"
                     /translation="MINLNEFATFCAIADTGSFSQAAEKLQISKALVTKHLLDLEQSL
                     GVKLVHRTTRKVGLTAAGAIFHQRCRRLLEDAGGMVEEIKRFRTSLGGHVKVSAAIAF
                     GRLHLAPLIARFLQQYPDVSVELTLTDRFADLITEGQDVVIRAAQEPRLSSLVARRLA
                     PWRWVLCAAPSYLARHPAPHVPRDLAGHNCLLYSSNTRGEWKFTHAEGAETVRVKGNF
                     KANNADGVLQGTLAGLGIAAITTMAAADEIRAGRLVRLLPDYLLPEGMLYASYLPNPT
                     MAQCVQTFVHFLEAEFANQAPWERGLDFPRWESPAAA"
     gene            5592..6572
                     /locus_tag="VGC69_15240"
     CDS             5592..6572
                     /locus_tag="VGC69_15240"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296683.1"
                     /translation="MNQRLFSRWARLFALTASLFAASAHADGYPDHPIHIIVPYSPGG
                     SSDVPMRVIAQQMSKQMGQSIIIENKPGDGSILGTQYVSHAAPDGYTLLLASNPQAIG
                     ATLYKSRLTFDPINDFEPISLFTREPGVLVVNPNLPVKTLGEFIDYVKAHPGQVDFAS
                     SGNGSAQHLFMEMFLARSGLKMVHIPYRGSAPAATDVVGGQVPAIMPGLSAMISYIRD
                     NRLRALAVTGDTRSPLLPDVPTLAESGFPGFSAYVWSGLVAPKNTPRPIIDKLNKELR
                     AAVNAPEVQAFIAKQSLEAITDTPEQLKTFMDDETRQWADVIAKAGVTVN"
BASE COUNT         1209 a         2261 c         2021 g         1282 t
ORIGIN      
        1 gcacgacaac catatagggc agcatcaatc cgccttcctt gggcagcgag atatcgacgt
       61 tcttgacgcc gtcgcgcttc atagcccaga tgcgggcaag ataatacggc gccgccacga
      121 cctcgccacg cgtgagcagc tggttgagct gggccagcga ggtatagacc accgacgcat
      181 tggccacctg cttcttgaac atcgggatgc tggccgagat gtccttctcg tcgccgccca
      241 tcgcatgcga caggatggtc aggtggtagg tggacgaata gatcggttgc gtgaccgcga
      301 tctggccctt ccacttggga ttgcccaagt cggaccacga ctgcatttcg gagggcttga
      361 cgtaatcggt gttgaacgcg attccgtagt acgtgaagta gaccggcgcg ccaatgagct
      421 tgccgtcggc cgctcgcatt ttgtactgct cgggcgtatc ggccagttgc gggatctcgc
      481 tgtcgctgaa ggtctcgagc agaccgtccc ggtagagccg ggccgcctcg aaatagccca
      541 ccagcacggc gttgtactgc ggcgccttgg cctgcgtgcg cacctgggcc tcggggttgg
      601 gcacctcggt aatgtttacc ttggtaccgg actcgttctg gaaaggcccg gcaaaagcat
      661 tgcgccaggt atcacccacg cccccgcccc aggtagccat ggaaacgctc tgcgcgctgg
      721 ccagggaagc cccacccagc gcggcctggc acaaaataac ggcggcggcc gtacgccact
      781 tgtgatacga agacatgatt tttccccttg ggtagactgc cgtcgagcac ggcccacgca
      841 gggcgcggca tcccggctgc tgaatgaatt gatgaaaagc atgaaccaaa tttcactttt
      901 catcaagcat gtttcataat tacgaaaatg cagtacacaa cctaaaaggc tatccagaat
      961 gatcaacccc atgctggacg actccccgga cgacacgcag cccgaagacg gccaacccac
     1021 caagatggac gccgtcgtcg gccagcgcat ccgggcactg agactggcgc gcgatctgtc
     1081 actcaaagag gttgccgtcc gctccggcct gtcgataggg ctgatcagcc aggtcgagcg
     1141 cggcatctcc tcgccatcgc tgcgcgtgct gggtgctctc agtggcgcgc tggaagttgc
     1201 catcgcctac tttttccccg agccggcaga gtctcaacgg gatccgaaat cgcccatcat
     1261 ccgcgtaaac cagcggcccg cgctgacctt ctggcgcacg ggcatcgcca agagcgtgct
     1321 cacacccgac gtccccggca acaacatcga cctggtgatg tacaccatca tcatcgagcc
     1381 caacggcacc accggcgacg agcccttcac ccacgatggc gaagaagccg gcgtggttct
     1441 cgaaggcacg ctggacctgc acatcggcga tcaggaatat gtgcttaacg agggcgacgg
     1501 ctttcgcttc tccagctcga tcccgcaccg cttcttcaac tccaccaagc gcgtggcccg
     1561 gatcatgtgg gtcaactgcc gggagcgcgc cgtctagccc ggattgaaac agccatagaa
     1621 atttcatttt tatgaattat cgttgttaaa aaatgaaatt cgatacatac ttccgcgcat
     1681 agccgccggc gccaggccga gtccattcaa acaatgcggg ggaaccgtgt ccgatccaat
     1741 cctgaccatc gaccatgtcg aaaagcgcta cgccggcttt catgccgtca agggggtcag
     1801 catggtggtc gagcgaggtg aattcctgac cctgctcggc ccttcgggct gcggcaagac
     1861 cacgctgttg cgcatgatcg ccggcctgga gcagcccagc agcggggaaa tcctcatcgg
     1921 cggggcaccc gtgactcgca tgccgcccta taaacgcaac ctgggcatgg tgttccagaa
     1981 cctggccttg tttccccacc tgaccgtggg cgagaacgtg gccttcggcc tgaaggtgcg
     2041 ccacaccgat acggccgcca ttgcaccggc cgtgacctcg gcgctgggcc tggtggggct
     2101 ggaccatctt gctgaccgcg ccgtgcacca gctcagcggc ggccagcgcc aacgcgtggc
     2161 actggcgcgc gcgttggcgg tgcggcccga ggtactgctg ctcgacgagc cgctcagtgc
     2221 gctggacatg aaactgcgtc gccagctgca agtggaactc aagcaattgc agcgccaggt
     2281 cggcaccacc ttcatcttcg tcacgcacga ccaggaagaa gcgctgagca tgagcgatcg
     2341 catcgccgtc atgaacgatg gccgagtcga acaactggcc tcggcgcagg aggtctacac
     2401 ccgcccggcc accctgttcg ccgcgcggtt cgtgggagac aacaatgtat tgaccgcccg
     2461 cgccggcgat gtcggcacgg atccaggcaa gctttacttt cccgcgctgg gcatggccag
     2521 gccggtaccc ggcctggcat cgctggctcc cgggcaggaa gtgcacgtcg cggtgcgccc
     2581 tgaacacata cgcgtacaga ccgccgagac aggaaccgcc gtcgtcgaga accaggtgta
     2641 ctccggctcg tccgtgcgcc tggcgctgcg cacggcgacc gacacgattc tggcgacaat
     2701 cgccgccgat gccacgacgg tggcgtccta tgccgtcggc acgcgcgtgc acatcgattg
     2761 gcccgatacc gcctgcaccg tgatccccgc cgccgcggga gccccactgt gaagctcaac
     2821 cggctcgcct tgctgctgtt gctgccggcc ggactcgtga tggccgtctt cgtcggcggc
     2881 ctggtctacc tggcatggca atcgctcgtc accccaaccg ggttggggct gcagaactac
     2941 gcggacttct tccagcgcca tgactacgtg ctggtgctgc tgcgcacgct ggaaatcgcg
     3001 gtcatcacga cggcagtcac actgctcatc agctacccgg ccgcctacgc cattgcccgc
     3061 gcgggcaagc ggcgcaacgc gctgctgctg ctggtgatcc tgccctggct ggtcagcctg
     3121 gtggtgcgca cctacgggtg ggtggtgctg ctgggcaatc gcggcacact gaattcgctg
     3181 cttgcctggc tgggcgcgac cgacggcccc atccgcctgc tgtttaacca gaccggcgtg
     3241 atcatcggac tggtgcacgt cttctgcccc ttcatgattc tttcgatact gacggtgttc
     3301 atgcacaccg aacgcgtgct gcaggaagcc agcgcgatgc tgggcgccaa tccactgcag
     3361 accttcctgc gcgtcgtcgg ccccctgtcg ctgcccggcg tgatctcggg ctgcacgatc
     3421 gtgttcctgc tgtccaccgg cgcggtcatt acaccgctgc tgctaggcgg accgcgcaac
     3481 gccatgctgg ctacgcagat ctaccaagac gtcttccagc tgttcaattt tcccaaggct
     3541 gccgccatgg cgttcctcct gaccctgtgc gccgcgatcc tggtatggcc gctgcaggtg
     3601 ctcgaaaagc gcatgatgcg ccacatgaag ggagcgcaat aatgatcgcc tggctcggcc
     3661 gatccgcatg gcgcgtactg cttgtgctga tattcgcgtt cctgcccgcc cccatcgtcg
     3721 tggtggtgct gtcctcgttc tcgcccacgg gataccttac ctttccgccc aaaggaatct
     3781 cgctgcgctg gtacgaagaa tttctgagca gcggcgactg gctgcacgcg ctggcgatca
     3841 gcgcggtact ggctgtcgcc gttgccgtgg taaccacggc actgggcttc atggcgggcc
     3901 tcgccaccag ccgggtgaaa ttccccggcc gcggcgcatt cggcctgctg atgctgtctc
     3961 cgctgctgtt tccgcacgcg gccatcgccg tcgccctatt gggcattctg tcctggcgca
     4021 acgcggtcgg cacttaccag ggtatcttcc tggcgcacgc catcctgtgc atgccctttg
     4081 cttaccggcc gctgctgaac tcgatgcgca agctcgacct cgccatggaa gaagcggcca
     4141 tgatgctggg cgccaccccg tcgcagatct tccggctggt gactctgccg ctgctcaagc
     4201 ccggcctggt caccgcgctg ctgttcagtt tcatcatctc gttcgatgaa gtaaccgtca
     4261 cggcattcct gctggggccg catgtcacga cactgccgac gcggatcttt tcattcgtcc
     4321 aggaaagcgc ctcgccggtg atcgccgcga tttcgacctt cctggtgatc tttacgctgc
     4381 tcatcgtcgt gctgctggat cggctggtgg ggatcgaact gtttgtggag accgaacgct
     4441 gaccccagcg aacacgaaat tccgatttga ctgaaagggt ttttccaacc ccttgatatc
     4501 ttgttagatt gtccgttcag acccaaatgc gctttaacat ctgaaccgac atgatcaatc
     4561 tcaacgagtt cgccaccttt tgcgccatcg ccgacacggg cagcttttcc caggcggcgg
     4621 agaagctgca gatatccaag gcgctggtca ccaagcatct cctcgacctg gaacagtcgc
     4681 ttggggtaaa gctcgtgcat cgcaccacgc gcaaggtggg gctgaccgcc gccggcgcaa
     4741 tttttcacca acgctgccgg cggctgctgg aagacgccgg cggcatggtc gaggaaataa
     4801 agcgctttcg cacctcgctg ggcggccatg tgaaggtcag cgccgcgatc gcgttcggcc
     4861 ggctgcatct ggcgccgctg atcgcgcggt ttctacagca atacccggat gtctcggtcg
     4921 agttgacgct gacggaccgc tttgccgacc tgatcaccga aggccaggac gtggtcatcc
     4981 gcgcggcgca ggagccgcgc ctgtcgtcgc tggtcgcgcg gcggctggcg ccgtggcgct
     5041 gggtgctgtg tgccgcgcca tcctatctgg cccggcatcc cgcgccgcac gtgccccggg
     5101 acttggccgg ccacaactgt cttctgtaca gctccaatac gcgcggcgaa tggaaattca
     5161 ctcacgccga aggcgccgag acggtgcggg tcaagggaaa tttcaaggcc aacaacgccg
     5221 acggcgtgct gcaaggcacg cttgccggcc tgggcatcgc ggccatcacg accatggcgg
     5281 ccgccgacga gatccgcgcc ggacggctgg tgcggctgct gccggattac ctgcttcccg
     5341 aaggcatgct gtacgcctcg tatctgccca accccaccat ggcgcaatgc gtgcagacgt
     5401 tcgtgcactt cctggaagcc gagttcgcca atcaggcgcc ttgggagcgc ggattggatt
     5461 ttcccagatg ggagagcccc gccgcggctt gaacagactg ttcacgagat catattgtca
     5521 attgcgcgag gctgacgcaa catcggcaac acacacggca gatcacatgc cggcacctca
     5581 aggggaattt cgtgaaccaa cgtctttttt cgcgctgggc gcgcttgttt gcgctcaccg
     5641 ccagcctttt cgccgcgtcg gcgcatgccg acggctatcc ggaccatccc atccacatca
     5701 tcgtgcccta ctcgccgggg ggctcgtccg acgtccccat gcgcgtgatc gcgcagcaga
     5761 tgtccaagca gatgggacag agcatcatca tcgagaacaa gcccggcgac ggctccatac
     5821 tgggtacgca gtacgtttcc cacgccgcgc cggacggcta tacgctgctg ctcgcatcca
     5881 atccgcaggc catcggcgcc accttgtaca agagccgcct gacgttcgac cccatcaacg
     5941 atttcgagcc catttcgctc ttcacccggg aacccggcgt tctagtggtt aacccgaatc
     6001 tgccggtgaa gacgctgggc gagttcatcg actacgtcaa ggcgcatccc gggcaagtcg
     6061 atttcgcgtc ttcgggcaat ggcagcgcgc agcacctttt catggaaatg ttcctggcgc
     6121 gcagcggctt gaagatggtg cacattccct atcgcggcag cgcccccgcc gccacggatg
     6181 tcgtgggcgg ccaggtgccg gcgatcatgc cgggcttgtc ggccatgatc tcgtacatcc
     6241 gcgacaaccg cctgcgggcc ctggcggtga ccggcgacac ccgctcgccg ctgctgcccg
     6301 acgtgcccac gctggccgag tcggggttcc ccggattctc ggcctacgta tggtccggcc
     6361 tggtcgcgcc gaagaacacg ccgcgcccca tcatcgacaa gctcaacaag gaattgcgcg
     6421 ccgcggtcaa tgcgcccgag gttcaggcgt tcatcgccaa gcagtccctg gaagccatca
     6481 ccgacacgcc ggaacagctc aagactttca tggacgacga gacgcggcag tgggccgatg
     6541 tcatcgccaa ggccggcgta acggtgaact gatccggggc gcgcctcacc cccacgttca
     6601 aagagaccta catgaccatt acacgctacg cgacccgcgc ctatgccgac ggcatcgtcc
     6661 acgcgcctta tgtgcgcgct ggaaactgga tattcggcac cggcctgcgc gccacgggcc
     6721 tcgatgggct gctggatccc gaggtcgccc gcccggggcg ccccctgggc ctg
//
LOCUS       DASWPO010000291        31881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1586609, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000291 DASWPO010000000
VERSION     DASWPO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 31881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31881
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586609"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGC69_15245"
     CDS             <1..382
                     /locus_tag="VGC69_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0296684.1"
                     /translation="GAVTMTLAYNGRIDTAVKQDHKPFKIVWDGQVYMYEWMGVPAGA
                     KHGDEAKKILAFASLPEHLATFTKFLPYAPPRMKALPLVAKDRLSDLPTAPDNFKHAL
                     KFDSAFWVDHGDEINKRFMTWLAK"
     gene            404..1657
                     /locus_tag="VGC69_15250"
     CDS             404..1657
                     /locus_tag="VGC69_15250"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296685.1"
                     /translation="MTQAAALVPAPGTPGSGDAKSWRRAQRRSARDALLLALPLLLFL
                     LVTFVVPIALLLLRGVENPEVPGAFPRLSQALAQWNGEGVPDEAAFAAVGSAMSDASQ
                     AGTLGTAARRLNFYQPEFRSLLMRSARNLPETAPPAWKPALIGIDPRWGEHETWRLLR
                     RGAQSPTPDYLLSALDKEVNARGAVVSVPDDEAVYLRAFGRTVWISVLVTLLCIVLGY
                     PVAWRLATLPPKQSGRLMLCVIIPFWTSLLVRTTAWYVMLQPKGIINSLLMSAGLISQ
                     PLPLMFNRTGVLIGMVHVLLPYMILAIYSVMKSVPPTYMRAAQSLGAHPALAFFRIYL
                     PQTLPGVGAGCFLVFVLSLGYYITPALLGGAGDEMISQLIASQTNELLNWGLAGALSA
                     YLVIFTVIFYLIFNRLVGIDRLRFG"
     gene            1669..2511
                     /locus_tag="VGC69_15255"
     CDS             1669..2511
                     /locus_tag="VGC69_15255"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296686.1"
                     /translation="MHIPSSPPDTRPLSTRISLKWVNFHTAALLLFLVAPVLAIIPLS
                     FNAGSYFSYPMSGFSLRWYEKALFSPDWQLAFTNSLGIGLVATVVATVLGTLAALGLS
                     RPNFPLRSLIMPVLISPMIIPLIVIAAGFYLVFAPIGLTNSYAGVILAHAVLGTPFVV
                     ITVTASLLSFDRNFMRAASGLGASPWAAFRRVTLPLILPGVATGAVFAFATSFDEVVV
                     ILFIGGPDQRTVPRQMWSGIRDAIDPSILAIATLLIVFAIALFVSMNWLRSRAQAANA
                     VLTE"
     gene            2513..3703
                     /locus_tag="VGC69_15260"
     CDS             2513..3703
                     /locus_tag="VGC69_15260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0296687.1"
                     /translation="MRLNRIQTIGELRRLARRRLPRIVYDYIEGGAGDERGLAASSRQ
                     YADYRIAPRYLVDVSARTQETRLFGRAFKSPFGIAPTGYAGIYRAKGEEILAAAASAA
                     GIPCIVSGSSVASIETVSRVAGGGHLWFQLYAARDTRISIDLLRRAKDCGIETLVVTV
                     DLPIFGRRYRDVRNGFQIPPRISPSLIWDAASHPGWTLDYLKAGGIPAPGNWIPYARE
                     GATPVEVATVMHENTTAPLRWSDIETFRRQWRGNLVLKGILHPEDAVKAAAIGVDGLI
                     VSNHGGRQFDRAPTATEALPAIVAAVGGRLTVMADGGITQGSDVLVAFALGAKFVFTG
                     RATLWGLAAAGLPGVDRALSILRDEVHAGLAHIGALGMPDVNLATARPGTAWACVCPR
                     AWTG"
     gene            complement(3781..4863)
                     /locus_tag="VGC69_15265"
     CDS             complement(3781..4863)
                     /locus_tag="VGC69_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0296688.1"
                     /translation="MTSLPAFGFIWPGMLWLLVLVPLLAGLYVWHDARRRGPTARYPA
                     LRMAGLAAARGAGRRRHLPPALMLLGLAALAVAVARPQADMALPSHLETVILAVDMSG
                     SMKADDVKPSRFRAAQQIAKAFLDEQPLSVSVGVVAVAGTAAVAQPPTHKKDDVAAAL
                     DRLQPQRGSALGSGIAIALSTLLPQMSADTQRFMNSDNTKPPGKTGSAGKAAGAVPPG
                     GAASEAAVPGSYASGAVVLLSDGEANIGPDTLRAAQFAADHGVRVYTVGIGTPQGTVL
                     KLEGMSARVKLDDESLKQVAEITGAEYFRLEDAAELKKVYRTLSARLSFDKRAQVEIS
                     ALFAALGALLAICAAFLSLWWYGRVL"
     gene            complement(4860..5849)
                     /locus_tag="VGC69_15270"
     CDS             complement(4860..5849)
                     /locus_tag="VGC69_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0296689.1"
                     /translation="MFGWRRASRRRGASAAGEATAPPGEALPGTRADALLRRLEWTVV
                     RRLDGLLQGDYRTLLRGFGLDLADLREYQPGDDVRHMDWNVTARLQAPHLREFQEDRE
                     VSAWFLLDLSGSVDFGSGGVRKRALLTEFTAVMARLLIRHGNRVGAVLYGGAQGDRFP
                     VIPARPGRRHLLHLVDRMNATPAASRGVTQLSELLAHAQAVIGRRSVLFVVSDFISEP
                     GWERPLAMLAQRHEVVAVRLIDPLETALPDLGLVLMQDAETGEQLFVDTHDAAFRRRF
                     AAAARTREADLGLAFARAGVDSLALTTDARLDLALLEFARHRRRGANAGGRRR"
     gene            complement(5827..6843)
                     /locus_tag="VGC69_15275"
     CDS             complement(5827..6843)
                     /locus_tag="VGC69_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0296690.1"
                     /translation="MNDLSMTDAAGSTELMERLLYEVKRVVVGQDHFLERVLVAMLAR
                     GHLLVEGMPGLAKTLTVNTLAKTMRGSFKRIQFTPDLLPADLVGTRMYNQRTGEFSTV
                     LGPVFANLLLADEINRAPAKVQSALLEVMQERQVTIAGETHLVPKPFLVMATQNPIET
                     EGTYPLPEAQTDRFMMKVLVDYPNEEEEAVIVDRVIGPAIAVNALATPEQLARLQDEC
                     RRIYVDPSLIQYAVRVVAATRKPDRHGLDELARYVSFGASPRATIGLIEGARALALLR
                     GRRYALPEDVIDLVPDVLRHRIALSYEALTDGVSADQFIARVLQAVPAPERPLESHVR
                     VAAS"
     gene            complement(6855..7745)
                     /locus_tag="VGC69_15280"
     CDS             complement(6855..7745)
                     /locus_tag="VGC69_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0296691.1"
                     /translation="MKRGAVYGWTAGAAALLVAAAVAVAWLALPRPHALTQADIDRAV
                     LHTLQTKPLPSQSARAANAVLQSVVKISGYDDAAAAPARIPNEGRRLLKQAAPPAAAS
                     HAGKDEKPVPGEGKPTPSNIGSGVVVTEDGTILTNYHVIAGADRLTATFYDGSESDLT
                     VVSAMPEKDLAVLHAKILPDDLPAATLGSSRDLAPGDGVVAIGFPFGIGPSVSAGVVS
                     GLDREFESAEDKQDLDHMIQFDAAANPGNSGGPLVNMNGEVVGIVTAILNPTKSRTFI
                     GIGFAATIEDAGMAVGLSPF"
     gene            7821..8816
                     /locus_tag="VGC69_15285"
     CDS             7821..8816
                     /locus_tag="VGC69_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0296692.1"
                     /translation="MRFLWPEILWSLLLLPLLAAAYFWLLARRKRAALVYASLALPRA
                     ALGPRQRLRRHIPPLFFLLALATALLACARPSATVTLPSDTFTLILVMDVSRSMLATD
                     IEPTRIAAAQQAARTFIEGLPNSARLGIVSFSSSATLVQPPTDDRRDMLDAIDRFQLQ
                     IGTATGSGLLLALATLFPDDGINLDGLPPDDTRPERPPAEPGSYTHGAVILLTDGRRT
                     AGPDPLDAARLAAERGVRIYTVGFGTESAGPVSGDAWSYFMQLDAATLRTVAKITNAD
                     YFQASSANDLSQVYRNLNTRFALERKETEISAILAAAAALLLVLACGLSILWFRR"
     gene            complement(8843..9319)
                     /locus_tag="VGC69_15290"
     CDS             complement(8843..9319)
                     /locus_tag="VGC69_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0296693.1"
                     /translation="MHTSLRLAIAAALACPAIAASAAGLPSHPYLTLAAAQQVAAAAQ
                     ARAQAQGWPCVISVVDAEGLPIVLLRMDDAAVLAGVELAPGKARTAALFRRPSGALED
                     AINGTRPAAATARGFVMMRGGVPLVVDKQIVGAIGVSADIPQHDQDIAEAGAAALK"
     gene            9434..10315
                     /locus_tag="VGC69_15295"
     CDS             9434..10315
                     /locus_tag="VGC69_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296694.1"
                     /translation="MELRHLRYFLAVAEHRHFAHAAESLGISPPTLTVQIKEIERTLQ
                     ARLFERARSGVSLTAAGEAFLAEARATLRQFDLAVSTGRRAGRGQLGHVALGYVGSAV
                     FSGILQAQAQAFRKAWPDVLLSVAELPMEQLPAMLEQGRVDIAFVRLPMRIPALLDSH
                     VLVRDRFCVALPGAHPLAAGAGALRPRDLARETFIVPEQDMGLREVARRGRFTPRIGA
                     VPGSLLAVLTQVSVGAGAAVVPDVLTRTVNLPHVAFRALAGRPIVSEVAAVFRRAERA
                     PAVRNLIGQIVRTAPGA"
     gene            10390..11499
                     /locus_tag="VGC69_15300"
     CDS             10390..11499
                     /locus_tag="VGC69_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY0296695.1"
                     /translation="MKSRAAVAFEAGKPLQIVEIDVEPPRKGEVLIKITHTGVCHTDA
                     FTLSGDDPEGLFPVVLGHEGAGIVVEVGAGVTSVKPGDHVIPLYTAECGECLFCKSGK
                     TNLCVAVRATQGKGVMPDGTSRFSYQGKPIYHYMGCSTFSEYSVVAEVSLAKINPDAN
                     PEHVCLLGCGVTTGIGAVHNTAKVQPGDTVAVFGLGGIGLAVLQGARQAKAARIIAVD
                     TNASKFELAKTFGATDCVNPKDYDKPIQQVIVEMTGWGVDHSFECIGNVEVMRAALEC
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRTWKGTAFGGVKGRSELPGMVEDAMQGK
                     IELAPFVTHTRHLDQINDAFELMHEGKSIRTVIRY"
     gene            11515..12348
                     /gene="fghA"
                     /locus_tag="VGC69_15305"
     CDS             11515..12348
                     /gene="fghA"
                     /locus_tag="VGC69_15305"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEY0296696.1"
                     /translation="MERIEQHASFGGRQEVYRHASTVLGCEMKFGIYLPEAALRGEPC
                     PVLYWLSGLTCTEQNFIAKAGAQEHAARHGIIVVAPDTSPRGDGVADDAAYDLGQGAG
                     FYLNATQAPWAAHYRMQDYVAEELPALIRRRFPANAAQGLFGHSMGGHGALVTALRHP
                     GRYRSVSAFSPIVAPSHVPWGEKAFTAYLGSDRGAWQAWDAVELIAGARERLPLLVDQ
                     GEADEFLAGQLRPELLQTACAAHGHPLTLRRHAGYDHSYYFIASFMADHFAHHAAALE
                     G"
     gene            complement(12388..13749)
                     /gene="pcaB"
                     /locus_tag="VGC69_15310"
     CDS             complement(12388..13749)
                     /gene="pcaB"
                     /locus_tag="VGC69_15310"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEY0296697.1"
                     /translation="MTSSIFQGATTVLDSVLFRDAFGTPAIREIFSDRALIERYIEVE
                     VALARAQAQCGVIPAEAAQVIADQSHFDALDLDLLRHETDIVGYPILPLVHQLVKLCG
                     EAGRYVHWGATTQDIMDTAVILQVRAALALVDDDIESLRGILADLSRRHRDTPMAGRT
                     HLQQALPVTFGYKAAIWLAMFDRHAERLVQLRPRVLVGQFAGAAGTLASLGDRGLDVQ
                     RALMSELDLGVPATTWHVARDGLAEAVNFLALVTGSLGKIALDVMLMASTEFAEVYEP
                     FVTGRGASSTMPQKRNPISSELMLAASKAVRQHAGLMLDAMVQDFERATGPWHAEWMA
                     LPESFVLTAGALNQAKFMLGGLIVDDARMRQNLGISAGLIVAEAVMMELAPHTGRQQA
                     HDLVYAACRMVNEQGGTLADALQTIPEVARHFDRAALERLTSPENYLGMAPRMVDAVL
                     AGR"
     gene            complement(13797..14972)
                     /locus_tag="VGC69_15315"
     CDS             complement(13797..14972)
                     /locus_tag="VGC69_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0296698.1"
                     /translation="MKITAVREISVPMQGNVANALVNFSEHDISLVALVSDVIRHGKP
                     VIGYAFDSIGRYAQGGILRDRMIPRLLKADPQSLLDASGLRLDPGKVLATVMRNEKPG
                     GHGDRAAAAGALELAAWDLNAKLDDEPAHVGIARRYGRQGKTGGVDVYAAGGYYYPDA
                     GDGTARLRDELLQYQDMGFQAFKMKIGGADLATDLKRIEAALSVAGEGGRLSVDVNGR
                     YDLEAALACARAIEPYQLRWYEEVGDPLDYDLNRRLIEAYPLPVATGENLFSVQDAKN
                     LILFGGMRPGKDIFQMDAGLCYGLGEYARMLALLESHGFNRAQAYPHGGHLINLHIAV
                     GLELGGCEAYPGVFPPFGGYPEGCGLGGGLVRPTDAPGFGLEEKTNLRPWLEKLAQG"
     gene            complement(15046..16371)
                     /locus_tag="VGC69_15320"
     CDS             complement(15046..16371)
                     /locus_tag="VGC69_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEY0296699.1"
                     /translation="MAIQPAAIPASPAASSSPFYTRLGFQITAGIVLGVALGFIEPRL
                     GMSVKVLGDIFLRLIKTVVAPLVFLNLVLGILSAGDLKKVGKIGLKALIYFEVVSSIV
                     LLISMVLANYSGVGGGMSLHPDPAAHAAAVASYGKATTQGFDVFLMSMTPDSFVGALT
                     GSSLLQVVVLAIAFGAAVLALSAKTRESVEGAFSTMSECFFKLTDMVMRFAPIGAFGA
                     IAYAVAASGMQVILSLGYLLLVMYAMLAFVVVVLFGVILRCFGMNIFHFLRYFKSEIG
                     ILFATGSSESAFPRLMEKLQALGCSKQTVSLVLPTGYAFNLDGTSVYLPLCVMFLANA
                     YGVPLTWAHQLGLLLIMLITSKGAATVSGGTFIVFAATVTASGILPIEGLPLLFGINR
                     FTSQAVCICNAMGNSVAAVVVAKLNGDFDERAAKAEYHRALGRPIDAPL"
     gene            16594..17331
                     /locus_tag="VGC69_15325"
     CDS             16594..17331
                     /locus_tag="VGC69_15325"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296700.1"
                     /translation="MASPRYVELAQELIRQIQSGHLAVGASLPNEVDLSQQYGVSRST
                     LRSALRMVQDLGLISRRKRAGIRVEAAKPRTAYERSLSSLEDLMQYATVTERHVQSTQ
                     LVVCDAPLARKLECAVLQKWLCVAMLRVDMQKPDEPLCWTDVYLDPEIGEGLGPQLLS
                     STGLICEMIEARYGCKVAEVRQEIRAVGVPAHAAQALGVAQDSHALEVTRRYLGEQGH
                     PFEITISVFPADRFAYGLVLRRHGHGV"
     gene            complement(17355..19661)
                     /locus_tag="VGC69_15330"
     CDS             complement(17355..19661)
                     /locus_tag="VGC69_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY0296701.1"
                     /translation="MGTDNSNRQAALNYHEFPKPGKLSVNASKPLITQRDLALAYTPG
                     VAAACEEIVANPMDAFRYTGRGNLVGVITNGTAVLGLGNIGALASKPVMEGKAVLFKK
                     FAGIDVFDIEINESDPDKLVEIIAGLEPTFGGINLEDIKAPECFTVERKLRERMKIPV
                     FHDDQHGTAITVCAAFINGLKVVGKDIKEIKVVTSGAGAAALACLDLLVDLGLPIEHI
                     WATDIEGVVYKGRTTLMDPDKERFAQETAQRKLAEVIEGADVFLGLSAGGVLKPEMVK
                     VMAPNPLILALANPSPEIRPEDAHAVRDDVVMATGRSDYPNQVNNVLCFPYIFRGALD
                     VGATTITREMEKAAVRAIAGLAQEEQNEVVAEAYGTYDICFGREYFIPKPFDPRLIAR
                     IAPAVARAAMDDGVATRPIADLDAYAEQLQQFVYHTGAFMTPVFGAVKRIVREGGKAR
                     IVFSEGEDERVLRAVQVVVDEKLARPILVGRPQVLLSRIEKYGLRLKLGEDVEVTNPE
                     YDERFHQYWTTYWELMCRRGITKEMARVEMRRRMTLIGAMMVRLGEADGMVCGSVGAY
                     HDHLRFVDEVIGKRPGAETYAAMNVLLLNERTIALVDTHVNDNPSAEQIAEFTIAAAR
                     QMSSLKLVPKVALLSRSNFGTGDWASAAKMREALALVRAQAPDIEIDGEMHGDCALDE
                     ALRMRILPSSTLKGSANLLVCPNVDSGNIAYNLLKATAGGNVAIGPFLLGANAPVSIL
                     TSSSTVRRIINMAALTVLEANRVDQTAA"
     gene            19782..20726
                     /locus_tag="VGC69_15335"
     CDS             19782..20726
                     /locus_tag="VGC69_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296702.1"
                     /translation="MAFDSGDVAHKLNNRLKMRHLALLLQIRQHGSLTRAAEHMAISQ
                     PALTNTLAELEGMFGAPLFERSARGMAPTLLGRVVLAHAQAMIQDLDHLVRDMDAVAG
                     GHTAHLHVGVTPFVPGRVLPAALQQAARDGRHLTVTVHEGPGELLLPQLQDHTLDIVV
                     GQASPALNLEGLGFDALYSQSPRLIANRRLAARLSRQRLDWPRLAGLDWIMSPSTSYL
                     REQVASLFVEAGLPAPRPVVETDVARLIGDMLAASERAVAILPADIAEELVRVAGVAI
                     IPYSFAWSLPPIGFFTRHSGTPRMAEQWFADALRKASH"
     gene            20862..22061
                     /locus_tag="VGC69_15340"
     CDS             20862..22061
                     /locus_tag="VGC69_15340"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0296703.1"
                     /translation="MTITRESARCFMPYPDAPVEHAASGPLAGLSFAVKDLFNVAGYP
                     TGGGNPHVLALSGIKAAHAATVAKLLGAGARFAGKTHTDELAFSMNGQNAHYGAPVNG
                     AAPDRITGGSSSGSASAVSNQFCDFAIGTDTGGSIRTPANHCNLYGIRPTHGRISLAG
                     ALDLAPGFDTCGFFTRDAATFKKVANVLLGADSAPLPAKPRLLLADDVFGLLSAAVLQ
                     SQQPALQKLRGQLGEMTPVQAGLPDFDTLYWAFRRIQGWEAWRVDGAMIEKYGLMLGS
                     GVKERFAWSKAVTQQQFEESSAIREQYRAMLGKLLGSNGVLVLPTMPGPAPLSSTPEA
                     ELDAYRNDAIRMLCLSGLAGFPQISLPLGAHDGAPLGISILGPAGSDASLVAIAARLV
                     DGVPLNA"
     gene            complement(22268..23749)
                     /locus_tag="VGC69_15345"
     CDS             complement(22268..23749)
                     /locus_tag="VGC69_15345"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY0296704.1"
                     /translation="MNAVPGAVARPEFRAFQPFQDAADAVDRLIEIYERNTAFLRRAF
                     QDALAGREQPGRARACYPAVRITVPSHDILDSRLSYGHVAEPGRYQTTITRPRLFRDY
                     LQRQISLLLQNHGGTVEVGESDLPIPLHFAFTDGANVEGQQIENLRRPLRDLFDVPDL
                     AVTDDAIVNGTWRDGDGGPRPLAPFTAPRVDYSLLRLQHYTATDPAHFQNFVLFTNYA
                     FYVDEFLAWARGLMASGQGEYERLVEPGNRVTPLGGAVCGDAPARQPQMPAYHLVRKG
                     HSGITLVNIGVGPSNAKTITDHVAVLRPHAWLMLGHCAGLRDTQRLGDYVLAHGYVRD
                     DHVLDADLPTWVPVPPLAEVQVALEQAVAEVAGLSGWDLKRIVRTGTVATIDDRNWEL
                     RNQSELVQRFAQSRAIALDMESATIAANGFRLRVPYGTLLCVSDKPLHGELKLPGMAS
                     AFYRKQVNQHLEIGIRALERLREMPLERLHSRKLRSFMETAFQ"
     gene            23944..26028
                     /locus_tag="VGC69_15350"
     CDS             23944..26028
                     /locus_tag="VGC69_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0296705.1"
                     /translation="MQTRDFFLPIPLRYTPLTLACLLAWSPGARAQDQPPAAAPAATL
                     ESVVVTGNPLGNDTIAQPTTVLEGRGLDLRRSATLGETLNGLPGVSTTTYGPMVGRPI
                     IRGMDGDRIRILQNGVGSLDASSLSYDHAVPQDPLSADRIEILRGPAALLYGGNAIGG
                     VVNTLDGRIPTEPINGVTGEALTSYGSAGEDRNGAVRLDAGDGQFAIHADAYARQTGN
                     LRIPGYAHSAAQRAIDSPDEPQAYGTLPNSDGSDHGGALGLAWTGDHGYLGLSYSGHD
                     SEYGSVAEEDVRIHMRQEKLGLAGELRDLDGWIKSVKITGALTNYEHREVDDGVVGTT
                     FKNRGYELRIEARHADIGPVSGSIGLQLGQSRFSALGDEALVPTTNTDTTALFIVEQL
                     KATDRLTLSAGARAEDTNLSPSAGAVERFDDSASRNIVASSASLGAVYQLNPAWSLAA
                     NGAYTERAPTFYELYANGVHDATGQYLVGNQNLGKERAWSADLALRYQQGTDKGSVGV
                     FYNRFSNYLAELNTGRYRDEDGNVVASTTPDALPEALYQGMPAEIYGVEAENRTRVLQ
                     RGGHTLDLDLSGDYTYTRSLSSGSSLPRIPPLRLRVALDYAWGPYDAGVGVTKAFAQH
                     RHADFDTPTPGYYSLDVNLGYRFKLGTTQWQAYARGVNLTNQTIRYATSILRDIAPEP
                     GRSLLVGLRGSF"
     gene            26248..26904
                     /locus_tag="VGC69_15355"
     CDS             26248..26904
                     /locus_tag="VGC69_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY0296706.1"
                     /translation="MTMPPTQKLHISLLAAAALAASLLYSPAHAAVEAEALPSLKGLS
                     TADQATVPNLRDRVVKMGLNAIGTLYRWGGDDPDEGFDCSGLVRFVFRQTAGMTLPRT
                     AREQHTAGQSVARQQLQPGDLVFFNGTVRTRIHITRGHHKSLRTVVSNVVSHVGIYIG
                     DNQFIHAPVSGERVRIDNLDASYWTKHYAGATRYLTPQAIARTAAAPAGLDDDTDDQP
                     "
     gene            complement(26979..27761)
                     /locus_tag="VGC69_15360"
     CDS             complement(26979..27761)
                     /locus_tag="VGC69_15360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296707.1"
                     /translation="MASPDTKAANPAGKVALVTGAGTGIGRAVALEFLARGYKVVLAG
                     RRAELLQATKAAAGAAADNALAVPADVAKEASVDALFAQLQAAYGRIDVLFNNAGRGA
                     PAVPIDELPASVWREVVDVNLTGMFLCAQGAIRLMKRQEPRGGRIINNGSISAHAPRP
                     MSVAYTATKHAVTGLTKSISLDGRRDHIACGQIDIGNAATEMTDRMAAGILQADGSVK
                     VEPRMDVAHVARAVAGMAELPLDTNVQFMTIMATDMPFVGRG"
     gene            27884..28516
                     /gene="gmk"
                     /locus_tag="VGC69_15365"
     CDS             27884..28516
                     /gene="gmk"
                     /locus_tag="VGC69_15365"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY0296708.1"
                     /translation="MANSPGNIFMVVAPSGAGKSSLVRALLDHDSSIEISISCTTRAP
                     RPGEVDGREYRFITPEAFLAMRDANTLLEWAEVHGNYYGTPSDRIDEATRAGRDVLLE
                     IDWQGARQVRQRFPKAIGVFILPPSIDELAKRLRARGQDPEEVISRRLLAAGSEIAHA
                     PECEYVIINQEFSAALAELIQIISVARLRFASQAARHAQLFAQLGLPPEH"
     gene            28590..28793
                     /gene="rpoZ"
                     /locus_tag="VGC69_15370"
     CDS             28590..28793
                     /gene="rpoZ"
                     /locus_tag="VGC69_15370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY0296709.1"
                     /translation="MARITVEDCLTKIPNRFKLTLAATYRARELAQGHAARIDSKDKP
                     TVTALREIAAGQTGAEMLRKVPT"
     gene            28827..31097
                     /locus_tag="VGC69_15375"
     CDS             28827..31097
                     /locus_tag="VGC69_15375"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY0296710.1"
                     /translation="MAFPGLKHASSGLLAALRAGSRLGLRSSGGKAEAVADATEPTSP
                     VASLAPLTEIINGYLSKADVEKVREAYRFADSAHLGQFRASGAPYISHPIAVTEICAG
                     WKLDANALSAALLHDVIEDQGITKPELAEKFGPEVAELVDGLTKLDRLDFASKAQQQA
                     ESFRKMLLAMARDVRVILIKLADRLHNMRTLDVVSPEKRRRVARETLDIYAPIAHRLG
                     LNLIYREFQDLCFAAMYPNRYQVLYRAVLAARGNRREVIGKIEDAVRAALPAAGIEAE
                     VTGREKTLYGIYRKMLAQKKSFTEVLDIYGFRVIVHTLPECYLALGTLHQLYRPVPGK
                     FKDYIAIPKMNGYQSLHTTLVGPYGTPVEFQFRTRDMHHVAEEGVASHWLYKESDTSL
                     NDLQKRTHQWLQSLLDIQSQTGDSGEFLEHVKVDLFPDAVYVFTPRGKIISLPRGATP
                     VDFAYAIHTDVGNQAVAAKVNGEFVPLRTELNSGDTIEIITSPASRPNAQWLNYVRTG
                     RARSEIRHYLRTVKYEESVNFGERLLQQTLDELHLSLPPADDPQWDKLARSTGASSRD
                     EILSDIGLGKRLAAVVARRFAPENSLLATTAAAMDEMTSARAAPILIQGNEGQAVQLA
                     PCCGPLPGDPIIAGMRLGQGLVVHTADCPVAQRAQAREPERWVPVAWDSQTSKHFSTR
                     LDVVTRNERGVLGRVAAEVAAADSNIIHVSMHDDATSTGLLHLTVQVDSRTHLAQVIR
                     AIRHVPQVQKIVRVKG"
     gene            complement(31163..>31881)
                     /locus_tag="VGC69_15380"
     CDS             complement(31163..>31881)
                     /locus_tag="VGC69_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0296711.1"
                     /translation="SVTRAAVLYAFRCLVGDDIPLNDGCLRPVTLVIPDGSMLSPGRE
                     AAVVAGNVETSQVVTDALFGALGTCAASQGTMNNFTFGNARYQYYETISGGTGAGPAR
                     IDATGPHEAGFHGTSVVQAHMTNSRLTDPEVLELRFPVRLESYSIREGSGGAGRYQGG
                     SGGIRRVRFLEDMTAAILSNNRRYAPFGLAGGSPGQVGRNYVERADGSVQELGPQDSA
                     QLHPGDVFVIETPGGGGYGE"
BASE COUNT         5219 a        11149 c        10407 g         5106 t
ORIGIN      
        1 cggcgcagtc acgatgacgc tggcctacaa cggccgcatc gacaccgccg tcaagcagga
       61 ccacaagccc ttcaagatcg tgtgggacgg ccaggtgtac atgtatgagt ggatgggcgt
      121 gccggcgggc gccaagcacg gcgacgaagc caagaaaatc ctggcattcg cctcgctgcc
      181 cgagcacctc gccaccttca ccaagttcct gccctacgcg ccgccgcgca tgaaggcgct
      241 gccgctggtc gccaaggacc gcctgagcga tctgccgacc gcaccggaca acttcaagca
      301 cgcgctgaag ttcgactccg cgttctgggt cgaccacggc gacgaaatca acaagcgctt
      361 catgacctgg ctggcgaaat aagccgcgcg acttaccgtc accatgacac aagccgctgc
      421 cctcgttccc gcgcccggca caccgggctc gggcgacgcc aaatcctggc gccgcgcgca
      481 acgccgttcg gcgcgcgacg cgttgctgtt ggccttgccc ttgttgctgt tcctgctggt
      541 gacgttcgtc gttcccatcg cgctgctgct gctgcgcggc gtggaaaacc ccgaagttcc
      601 cggggcgttt ccgcgcctgt cccaggcgct ggcgcagtgg aacggcgaag gcgtcccgga
      661 cgaggcggcg ttcgcggcgg tgggttcggc catgagcgac gcctcgcagg cggggacgct
      721 gggcacggcg gcgcgccggc tcaatttcta tcagcccgaa tttcgcagcc tgctgatgcg
      781 gtccgcgcgc aacttgccgg aaaccgcgcc gcccgcctgg aagccggccc tgatcggcat
      841 cgacccgcgc tggggcgaac atgaaacctg gcggctgttg cggcgcggcg cgcagtcgcc
      901 cacgcccgac tatctgcttt ccgcgctcga caaggaagtg aacgcgcgcg gcgccgtcgt
      961 ttccgtgccg gacgacgaag cggtctacct gcgcgcgttc ggccgcaccg tatggataag
     1021 cgtgctcgtc acgctgctct gcatcgtgct gggctatcct gtggcctggc ggctggccac
     1081 cttgccgccg aaacaaagcg gccgcctgat gctatgcgtc atcatcccgt tctggacgtc
     1141 gctgctggtg cgcacgaccg cttggtacgt gatgctgcag cccaagggca tcatcaacag
     1201 cctgctgatg agcgcaggcc tcatcagcca gccgctgccc ctgatgttca accggacggg
     1261 cgtgctgatc ggcatggtgc acgtgctgct gccctacatg atcctggcca tctactccgt
     1321 catgaaaagc gtgccgccta cctatatgcg cgcggcccag tcgctgggcg cgcatccggc
     1381 cctggccttt ttccgcatct acctgccgca gaccctgccg ggcgtgggcg cgggctgctt
     1441 cctggtgttc gtgctgtcgc tgggctacta catcacgccg gccctgctgg gcggcgccgg
     1501 cgatgaaatg atcagccagc tcatcgccag ccagaccaac gaactgttga attggggcct
     1561 ggcgggtgcg ctgtcggctt acctggtgat cttcaccgtg atcttctatc tgattttcaa
     1621 ccggctcgtc ggcatagacc ggctgcgatt cgggtgacgg gaaacgccat gcacattccc
     1681 agctcccccc cggacacacg gccgctttcc acccggatca gcctgaagtg ggtcaatttc
     1741 cacaccgcgg cgctgctgtt atttctggtg gcgccggtgc tggccatcat cccgctatcg
     1801 ttcaacgcgg gctcctattt ttcatatcca atgtcgggat tttcgctgcg ctggtatgaa
     1861 aaggccctgt tcagtccgga ttggcagctt gccttcacca acagcctggg catcgggctg
     1921 gtggccaccg tggtcgccac ggtgctgggc acgctggcgg cgctgggcct gtcccggccg
     1981 aatttcccgt tgcgctccct catcatgccg gtgctgattt cgcccatgat catcccgctc
     2041 atcgttatcg cagcgggctt ctatctggtg tttgcgccca tcgggctgac caattcgtat
     2101 gccggcgtca tattggccca tgcggtgctc ggcacgccct tcgtcgtcat taccgtgact
     2161 gcgtcgctgc tgtcgttcga ccgcaatttc atgcgcgccg cgagcgggct gggcgcttcg
     2221 ccctgggcgg cgttccggcg cgtgacgctg ccgctgattc tgccgggcgt ggctacgggc
     2281 gcggtattcg cgttcgccac ctcgttcgac gaggtggtcg tgatcctgtt catcggcggg
     2341 ccggaccagc gcaccgtgcc gcgccagatg tggagcggca tacgcgacgc catcgaccct
     2401 tcgatactgg cgatcgccac cctgctcatc gtgttcgcca tcgcgctgtt cgtctcgatg
     2461 aactggctgc gcagccgcgc gcaggccgcc aacgccgtgc tgaccgagtg atatgcgcct
     2521 gaaccgcatc cagaccatcg gcgaactgcg ccggcttgcg cgccgccggc tgccgcgcat
     2581 cgtctacgac tacatcgaag gcggcgccgg cgacgagcgc ggcctcgcgg catcctcccg
     2641 gcaatatgcc gattaccgca tcgccccgcg ctatctcgtc gacgtcagcg cgcgcaccca
     2701 ggaaacgagg ctgttcggcc gcgcattcaa aagcccgttc ggcatcgccc cgaccggcta
     2761 cgccggcata taccgcgcca agggagagga aatcctggct gcggccgcat cggcggccgg
     2821 cattccctgc attgtgtccg gttccagcgt cgcctcgatt gaaaccgtaa gccgcgtcgc
     2881 cggcggcggc catctctggt tccagctcta cgcggcgcgg gacaccagga tttccatcga
     2941 tctgctccgg cgcgccaagg attgcggcat cgaaacgctg gtcgtgacgg tcgatctgcc
     3001 gattttcggc cgccgctacc gcgacgtccg caacggtttc cagatcccgc cgcgcatttc
     3061 gccttcgttg atatgggacg ccgcgagcca tcccggctgg accctcgatt atctcaaggc
     3121 cggcggcatt cccgcgcctg gaaattggat tccctatgcc agggaaggcg caaccccggt
     3181 ggaagtcgcg accgtgatgc acgaaaacac gaccgcgccc ctgcgctgga gcgatatcga
     3241 gacgttccgg cgccaatggc gcggcaacct ggtgctcaag ggcattctgc atccggaaga
     3301 cgcggtgaag gcggccgcaa tcggcgtcga tggcttgatt gtctccaacc acggcggccg
     3361 ccagttcgac cgcgcgccga cggcgaccga agcgctgccc gccatcgtgg ccgccgtcgg
     3421 cggccggctg accgtcatgg cggacggcgg catcacccag ggctcggacg tcctggtcgc
     3481 cttcgcgctg ggcgcgaaat tcgtcttcac cggccgcgcc acgctgtggg gactggccgc
     3541 cgccgggctg cccggcgtcg accgcgcgct gtcgattctg cgcgacgaag tccatgccgg
     3601 gcttgcccat atcggcgcgc taggcatgcc ggacgtcaat ctagccaccg cacggcccgg
     3661 cacagcctgg gcgtgcgtgt gtccacgcgc ttggaccggc tgaatgtcct cgcaacagtc
     3721 tgcattcgga tttttatcga tagaccaggc gacggccgac ccgaaaacgg cgaggccgcc
     3781 ctacagcacc cgcccatacc accacagcga caggaaggcc gcgcagatcg ccaggagcgc
     3841 gcccagcgcg gcgaagagcg cggagatctc gacctgcgcg cgcttgtcga acgacagcct
     3901 ggccgagagc gtgcgataga cctttttcag ttcggcggcg tcttcgaggc gaaagtactc
     3961 ggcgcctgtg atctcggcga cctgcttgag cgattcatcg tcgagcttga cccgggccga
     4021 catgccttcc agcttgagca ccgtgccttg cggcgtgccg atgccgacgg tgtagacgcg
     4081 cacgccgtga tcggcggcga actgggcggc ccgcagggtg tcggggccga tgttggcctc
     4141 gccgtccgag agcagcacga cggctcccga ggcataggaa ccgggcaccg ccgcctcgct
     4201 ggccgccccg cccggcggca cggcgccggc ggccttgccg gcggagcctg tcttgcccgg
     4261 gggcttggtg ttgtccgagt tcatgaagcg ctgcgtgtcg gcgctcatct gcggcaacag
     4321 ggtcgagagc gcaatggcga tgccgctgcc cagggcggag ccgcgctgcg gctgcagacg
     4381 gtcgagcgcc gcggccacat cgtctttctt gtgggtgggc ggctgggcca cggccgcggt
     4441 gcccgccacc gcgaccacgc ccacgctcac ggacaggggc tgttcgtcga gaaaggcctt
     4501 ggcgatctgc tgcgcggcgc gaaaacggct gggcttgacg tcgtcggcct tcatgctgcc
     4561 cgacatgtcc acggccagga tcaccgtttc gagatgcgag ggcagcgcca tgtcggcctg
     4621 gggccgcgcg accgcgaccg ccagcgcggc caggcccagc agcatcagcg cgggcggcag
     4681 gtggcggcgg cggcccgcgc cgcgcgccgc ggcaagcccc gccatccgca gggccgggta
     4741 gcgcgcggtc ggcccgcgcc tgcgcgcgtc gtgccacaca tagagtcccg ccagcaaggg
     4801 aacgagcaca agcagccaca gcatgccggg ccagataaag ccgaaagcgg gcaaggacgt
     4861 catcgccggc gtcccccggc gttcgcgccg cgccgcctgt gccgggcgaa ttccagcagg
     4921 gcgagatcca ggcgcgcatc ggtcgtcaac gccaggctat cgacgccggc gcgcgcgaag
     4981 gccaggccca ggtcggcctc gcgcgtccgg gcggccgcgg cgaagcgtct gcggaaagcc
     5041 gcgtcgtgcg tatccacgaa taactgctcg ccggtttcgg cgtcctgcat caagaccagg
     5101 ccgaggtcgg gcaaggccgt ttccagcggg tcgatcaagc gcacggcgac gacttcgtgg
     5161 cgctgcgcca gcatggccag cggcctttcc caaccgggtt cgctgatgaa gtcggacacg
     5221 acgaagagca ccgagcgccg gcctatcacc gcctgcgcgt gggccagcag ctcactgagc
     5281 tgcgtgacgc cgcgggatgc ggcgggcgtc gcgttcatgc ggtcgacgag gtgcagcaga
     5341 tggcgccgcc ccgggcgcgc cgggatgacc ggaaaccgat cgccctgggc tccgccataa
     5401 agcacggcgc cgacccggtt gccgtggcgg atcagcagcc gcgccatgac ggcggtgaac
     5461 tcggtcagca gcgcgcgctt gcgcacgccg cccgagccga aatccaccga gccgctcaaa
     5521 tccagcagga accaggccga gacttcgcgg tcttcctgga actcgcgcag atgcggggcc
     5581 tgcaggcgcg ccgtgacgtt ccagtccatg tggcgcacat cgtcgccggg ctggtattcg
     5641 cgcaggtcgg ccaggtccag accgaaccct cgcagcagcg tgcggtagtc gccctgcagc
     5701 aggccgtcga gccgccgcac gacggtccat tcgagccggc gcagcagcgc gtcggcccgc
     5761 gttccaggca aggcctcgcc gggcggcgcc gtggcctcgc ccgcggcgga cgcgccgcgc
     5821 cgccggctag ctcgccgcca cccgaacatg ggattccagc gggcgctcgg gcgccggcac
     5881 ggcctgcagc acgcgggcaa tgaactggtc cgcgctcacc ccgtcggtca aggcttcgta
     5941 ggaaagcgcg atgcgatggc gcagcacatc gggcacgagg tcgatcacgt cttcgggcag
     6001 cgcgtagcgg cggccgcgca gcagggccag ggcgcgcgcg ccttcgatga ggccgatggt
     6061 ggcgcgcggg ctggccccga aagagacgta gcgcgcgagc tcgtcgagcc cgtggcggtc
     6121 gggcttgcgc gtggcggcca ccacgcgcac cgcgtactgg atcaggctcg ggtccacgta
     6181 gatccggcgg cattcgtcct gcagccgcgc cagctgctcg ggggtggcca gggcgttcac
     6241 cgcgatggcg gggccgatga cgcggtcgac gatgacggct tcttcttcct cgttgggata
     6301 gtccaccagc accttcatca tgaagcggtc ggtctgcgcc tcgggcaagg gataggtgcc
     6361 ctcggtctcg atcgggttct gcgtcgccat gacgagaaac ggcttgggaa ccaggtgggt
     6421 ctcgcccgcg atggtgacct gtctttcctg catgacctcc agcagcgcgc tctgcacctt
     6481 ggccggcgct cggttgattt catcggccag cagcaggttg gcgaagaccg gccccagcac
     6541 ggtggagaac tcgcccgtgc gctggttgta catgcgcgtg ccgaccaggt cggcgggcag
     6601 gaggtccggc gtgaactgga tgcgcttgaa cgagccgcgc atggtcttgg ccagcgtgtt
     6661 caccgtcagc gtcttggcca ggcccggcat gccctcgacc agcaggtggc cgcgcgccag
     6721 catggccacc aggacccgct cgagaaagtg gtcctgcccc accaccacgc gcttgacctc
     6781 gtacagcagg cgctccatca gctcggtgct gcctgcggcg tccgtcatgg ataagtcgtt
     6841 catatatctc cgcgctagaa gggggaaagg cccacggcca tgccggcgtc ttcgatggtg
     6901 gcggcgaagc cgatgccgat gaaggtacgg ctcttggtgg ggttgaggat ggcggtgacg
     6961 atgcccacca cctccccgtt catgttcacc agcgggccgc cggaattgcc cgggttggcc
     7021 gcggcgtcga actggatcat gtggtcgaga tcctgcttgt cctcggccga ctcgaactcg
     7081 cggtcgagac ccgacaccac gccggccgac accgacgggc cgatgccgaa gggaaaaccg
     7141 atggccacga ccccgtcgcc gggcgcaaga tcgcggctgg agccgagcgt ggccgccggc
     7201 aggtcgtcgg gcaggatctt ggcgtgcagc acggcgaggt ccttttccgg catggcgctc
     7261 accacggtga gatcggattc ggagccgtcg tagaaagtgg ctgtcagccg gtcggcgccc
     7321 gcgatgacgt ggtagttggt gaggatggtg ccgtcttcgg tcacgaccac gcccgagccg
     7381 atattgctcg gcgtgggctt gccctcgccg gggacgggct tctcgtcctt gccggcatgg
     7441 gatgcagcgg cgggcggcgc cgcttgcttg agcagcctcc tgccttcatt gggaatcctg
     7501 gccggcgcgg cggcggcgtc gtcatagccg gaaatcttca ccaccgattg cagcaccgca
     7561 ttggccgccc gggccgattg cgacggcagg ggcttggtct gcaaggtatg gagcacagcg
     7621 cggtcgatgt cggcctgcgt cagggcatgc ggacgcggca gcgccagcca tgccacggcc
     7681 accgccgcgg caacaagcag cgcagctgcg cccgccgtcc atccgtagac cgctccccgt
     7741 ttcatcgcag atgccgaagt cccgtcccac tgtggaagta cagtaccatc gtcgcatcca
     7801 cgcgccaagg agcacttacc atgcggtttc tctggccgga aatactttgg tcgctgttac
     7861 tgctgcctct cctggcggct gcctacttct ggctgctggc gcggcgcaag agggcggccc
     7921 tcgtctacgc cagcctggcc ctgccgcgcg ccgccctggg cccgcggcag cggctgcggc
     7981 gccacattcc gcccctgttt ttcctgctgg cgctggcaac cgccctgctg gcctgtgccc
     8041 ggcccagcgc gaccgtcacc ctgccttccg acacgttcac gctgatactg gtcatggacg
     8101 tgtcgcgcag catgctggcg accgacatcg agcccacgcg catcgccgcc gcccagcagg
     8161 cggcgcgcac tttcatcgaa ggcctgccga acagcgcgcg cctgggcatc gtgtccttca
     8221 gcagctcggc gaccttggtg cagccgccca ccgacgaccg gcgcgacatg ctggacgcca
     8281 tcgaccgttt ccagctccag atcggcacgg ccaccggcag cggcctgctg ctcgcgctgg
     8341 ccaccctctt tcccgacgac ggcatcaacc tcgatgggct gccgcctgat gacacccggc
     8401 cggagcgccc gcctgccgaa cccggctcgt acacgcacgg cgccgtcatc ctgctgaccg
     8461 acggccggcg caccgccggg cccgaccccc tggacgccgc gcgcctcgcc gccgaacgcg
     8521 gagtgcgcat ctacaccgtg ggtttcggca ccgagtcggc cgggcccgtc agcggcgacg
     8581 cctggtcgta tttcatgcag ctcgatgccg ccacgctgcg gacggtggcc aagatcacca
     8641 acgccgacta tttccaggcg agctcggcca acgacttgag ccaggtctac cgcaatctca
     8701 acacccgctt tgcgctcgag cgcaaggaaa ccgagatcag cgccatcctg gccgcggcgg
     8761 cggccctgct gctggtgctg gcctgcgggc tgtcgatcct gtggttcagg cggtaatcct
     8821 taaataacca ggcctgccgc gcctatttca gcgcggcggc gccggcctcg gcgatgtcct
     8881 ggtcatgctg gggaatatcc gccgacacgc cgatcgcgcc gactatctgc ttgtccacca
     8941 ccaagggcac gccgccgcgc atcatcacga agccgcgcgc cgtggcggcg gcgggacggg
     9001 tgccgttgat cgcatcttca agcgcgccgc tcgggcggcg gaacagcgcg gccgtgcggg
     9061 ccttgccggg ggccagttcc acgcccgcga gcaccgcggc atcgtccatg cgcagcaaca
     9121 cgatgggcag gccctcggcg tcgaccaccg agatgacgca gggccagccc tgcgcctgcg
     9181 ccctggcctg cgcggcggcg gcgacttgct gggcggcggc cagcgtgagg tagggatgcg
     9241 agggaagccc ggcggccgag gcggcgatgg cggggcaagc cagcgccgcg gcaatggcca
     9301 gacgtaggga ggtatgcatg ggttcgatcc tggtgtgtct tggagaagca ccatttgagc
     9361 aagccccggc attttgttca ttaatgctat ttttcctgat aattaggttt tagctaatct
     9421 tgatgggggc acgatggagc tgcggcatct gcgatatttc ctggcggtgg ccgagcatcg
     9481 gcattttgcg cacgcggccg agagcctggg catttcgccg cccacgctca cggtgcagat
     9541 caaggaaatc gagcggacgc tgcaggcccg gctattcgag cgtgcccgca gcggcgtgtc
     9601 gctgaccgcc gccggcgagg ccttcctggc cgaggcgcgc gccaccctgc gacaattcga
     9661 cctggccgtc agcaccggcc ggcgcgcggg gcgcgggcaa ttgggccatg tcgcgctggg
     9721 ctacgtgggg tccgccgtgt tctcgggaat cttgcaggcc caggcccagg cattccgcaa
     9781 ggcatggccc gacgtgctgc tgagcgtggc cgagctgccc atggagcagt tgccggcgat
     9841 gctggagcaa ggccgggtcg acatcgcttt cgtgcggctg cccatgcgca ttcccgccct
     9901 cctggacagc cacgtgctgg tgcgcgaccg cttctgcgtc gcgctgcccg gcgcccatcc
     9961 gctggcggcc ggggcaggcg cgctgcgccc gcgcgacctg gcgcgcgaaa cctttatcgt
    10021 gcccgagcag gacatggggc tgcgcgaagt ggcgcggcgc ggccgcttca ccccgcgcat
    10081 cggcgcggtg ccgggcagcc tgctggcggt gctgacccag gtatcggtag gcgccggcgc
    10141 ggccgtggtg cccgatgtgc tgacccgcac ggtcaacctg ccccatgtcg cgttccgggc
    10201 gctggccgga cgccccatcg tgtcggaagt ggccgccgta ttccgccgcg ccgagcgcgc
    10261 gccggcggtg cgcaacctga tcggccagat cgtgcgcacg gcgcccggcg catgactgcg
    10321 cggcgcgcgc cgacgccgta cttgtttatg tggtagaaaa ggacggcatt cagaacagga
    10381 gtacacccca tgaaatcccg cgcagccgtg gccttcgagg ccggcaagcc cctgcaaatc
    10441 gtcgagatcg acgtcgagcc gcccaggaag ggcgaagtgc tgatcaagat cacccatacc
    10501 ggcgtttgcc ataccgatgc cttcaccctg tcgggcgacg atcccgaggg cctctttccc
    10561 gtcgtgctgg gccatgaagg cgccggcatc gtggtggagg tcggcgcagg cgtcaccagc
    10621 gtcaagcccg gcgaccacgt catcccgctc tacaccgccg agtgcggcga atgcctgttt
    10681 tgcaagtcgg gcaagaccaa tctgtgcgtg gccgtgcgcg ccacccaggg caagggcgtg
    10741 atgcccgacg gcacttcgcg cttttcctac cagggcaagc ccatctacca ctacatgggc
    10801 tgctcgacct tcagcgaata ctccgtggtg gccgaggtgt cgctggccaa gatcaacccc
    10861 gacgccaatc ccgagcacgt ctgcctgctg ggctgcggcg tgaccaccgg catcggcgcc
    10921 gtgcacaaca cggccaaggt ccagccgggc gacacggtgg ccgtgttcgg cctgggcggc
    10981 atcggcctgg cggtcctcca gggcgcgcgc caggccaagg ccgcgcgcat catcgccgtc
    11041 gacaccaatg cgtccaagtt cgagctggcc aagaccttcg gcgccaccga ttgcgtcaac
    11101 cccaaggact acgacaagcc catccagcag gtcatcgtcg agatgacggg ctggggcgtg
    11161 gaccattcct tcgagtgcat cggcaacgtc gaggtgatgc gcgccgcgct ggaatgcgcg
    11221 caccgcggct ggggccagtc ggtcatcatc ggcgtggccg gcgcgggcca ggaaatctcc
    11281 acccgcccct tccagctggt gacgggccgc acctggaaag gcacggcctt cggcggcgtc
    11341 aagggccgca gcgaactgcc cggcatggtc gaggacgcca tgcagggcaa gatcgagctc
    11401 gcgcccttcg tgacgcatac ccgccacctc gaccagatca acgacgcctt cgagctgatg
    11461 catgaaggca agtcgatccg caccgtcatt cgttattgac tacggctact gatcatggag
    11521 cgcatcgaac agcacgccag cttcggcggc cgccaggagg tgtaccggca cgcctccacc
    11581 gtgcttggct gcgagatgaa gttcggcatc tacctgcccg aggccgccct gcgcggcgag
    11641 ccctgccccg tgctctactg gctctcgggc ctgacctgca ccgaacagaa cttcatcgcc
    11701 aaggccggcg cccaggagca cgcggcgcgc cacggcatca tcgtggtcgc gcccgacacc
    11761 agcccgcgcg gcgacggggt ggccgacgac gcggcctacg acctgggcca gggcgcgggc
    11821 ttctacctca acgccacgca ggcgccctgg gccgcgcact accgcatgca ggactacgtg
    11881 gccgaggaac tgcccgcctt gatccggcgg cgcttccccg ccaatgcggc gcagggtctc
    11941 ttcggccatt cgatgggcgg ccatggcgcc ctggtgacgg cgctgcgcca tcccggccgc
    12001 taccggagcg tgtcggcgtt ttcgcccatc gtcgcgccct cgcacgtgcc ctggggcgaa
    12061 aaggcgttca ccgcctacct gggcagcgac cgcggcgcct ggcaggcctg ggacgccgtc
    12121 gaactcatcg ccggcgcgcg ggaacggctg ccgctgctgg tcgatcaggg cgaagcggac
    12181 gaattcctgg ctggacagct gcgtcccgaa ttgctgcaga ccgcctgtgc ggcgcacggc
    12241 catccgctca cgctgcgccg gcatgccggc tacgaccaca gctactactt catcgccagc
    12301 ttcatggccg accacttcgc ccatcacgcg gcggcgctcg agggctgact ccccggcctg
    12361 ggaaagcgcc caggccggcg ccccgcttta gcgccccgcc agaacggcgt ccaccatccg
    12421 gggcgccatg cccaggtagt tctcgggcga ggtcaagcgc tccagcgcgg cccggtcgaa
    12481 gtggcgggcg acttcgggaa tggtttgcag ggcgtcggcc agggtgccgc cctgctcgtt
    12541 gaccatgcgg caggccgcgt agacgagatc gtgcgcctgc tggcggcccg tatgcggcgc
    12601 cagttccatc atcaccgcct cggccacgat cagtccggcg gaaatgccca ggttctggcg
    12661 catgcgggca tcgtcgacga tcaggccgcc gagcatgaat tttgcctggt tgagcgcgcc
    12721 cgccgtcagg acgaagctct cgggcagcgc catccattcg gcatgccagg ggccggtggc
    12781 gcgctcgaag tcctgcacca tggcatcgag catcagtccg gcgtgctgac gcacggcctt
    12841 ggacgccgcc agcatcagtt cgctggagat cgggttgcgc ttctgcggca tcgtgctgct
    12901 ggcgccgcgc cccgtcacga acggctcgta cacctccgcg aattccgtcg aagccatcag
    12961 catgacgtcc agggcgatct tgcccagcga accggtcacc agggccagaa agttcaccgc
    13021 ctcggccagg ccgtcgcgcg ccacgtgcca ggtcgtggcg ggcacgccca ggtccagttc
    13081 cgacatcagc gcgcgctgca cgtccagccc gcggtcgccc agcgaagcca gcgtgcccgc
    13141 cgcgccggcg aactgcccca ccagcacgcg cgggcgcagc tggaccagcc gttcggcatg
    13201 gcggtcgaac atggccagcc agatggcggc cttgtagccg aaggtgacgg gcagcgcctg
    13261 ctgcaagtgg gtgcggccgg ccatgggcgt atcgcggtgg cggcgcgaca ggtcggcgag
    13321 gatgccgcgc agcgattcga tatcgtcgtc caccagcgcc agcgcggcgc gcacttgcag
    13381 gatcacggcg gtgtccatga tgtcctgcgt ggtggcgccc cagtgcacat agcggccggc
    13441 ttcgccgcac agtttcacca attggtgcac cagcggcagg atcgggtagc cgacgatgtc
    13501 cgtctcatgg cgcagcaggt ccaggtccag cgcatcgaaa tgcgactggt cggcgatgac
    13561 ctgggccgcc tcggcgggaa tcacgccgca ttgggcctgg gcgcgggcca gcgccacctc
    13621 gacctcgatg tagcgctcga tcagcgcgcg gtcgctgaag atctcgcgga tggccggcgt
    13681 gccaaaggca tcgcggaaca ggacggaatc gagcacggtg gtcgcgccct ggaagatcga
    13741 agaagtcatg tcgcaggttt tcctggcgta gggttgcggg cttgccgtcg cgggcatcag
    13801 ccctgggcca atttttccag ccacggccgc aaattggttt tttcctcgag gccgaagccc
    13861 ggcgcgtcgg tgggccgcac caggccgccg cccaggccgc agccttcggg atagccgccg
    13921 aagggcggga acacgcccgg gtaggcttca cagccgccca gctccagccc gacggcgata
    13981 tgcaggttga tgagatggcc gccatgcgga taggcctgcg cccggttgaa accgtgggat
    14041 tccagcagcg ccagcatgcg ggcatattcg cccagcccgt agcagaggcc ggcgtccatc
    14101 tggaagatgt ccttgcccgg gcgcatgccg ccgaacagga tcaggttttt cgcgtcctgc
    14161 accgaaaaca ggttttcgcc ggtggccacg ggcaaggggt aggcctcgat caggcggcga
    14221 ttgaggtcgt aatccagcgg gtcgcccact tcctcgtacc agcgcaactg atagggttcg
    14281 atcgcgcggg cgcaggccag ggccgcttcc aggtcgtagc ggccattgac gtcgaccgac
    14341 aggcggccgc cctcgcccgc caccgacaag gcagcctcga tacgcttgag gtcggtggcc
    14401 aggtccgccc cgccgatctt catcttgaat gcctggaaac ccatgtcctg gtactgcagc
    14461 agttcgtcac gcagccgcgc ggtgccatcg ccggcatccg ggtagtaata gccgccggcc
    14521 gcgtagacat ccacgccgcc ggtcttgcct tggcggccgt agcggcgcgc gatgccgaca
    14581 tgcgctggct cgtcgtcgag cttggcgttc aggtcccagg cggccagttc gagcgcgccg
    14641 gccgcggcgg cgcgatcgcc gtgcccgccg ggcttctcgt tgcgcatgac ggtggccagc
    14701 accttgccgg gatcgaggcg caggccgctt gcatcgagca gggattgcgg atcggccttg
    14761 agcaggcggg ggatcatccg gtcgcgcagg attccgccct gcgcgtagcg gccgatggag
    14821 tcgaacgcat agccgatgac cggcttgcca tggcgtatga cgtcgctgac cagggcgacc
    14881 agggaaatat cgtgctcgga aaagttgacc agcgcattgg cgacgttgcc ttgcatcggc
    14941 acggaaattt ctcggacggc ggtgatcttc atgaaacgaa ccttctagaa aaaacggaaa
    15001 aacggcggac cggcgcggcc agggcgccgg caggcggggc agcgttcaca gcggcgcgtc
    15061 gatcggccgg cccagcgcgc ggtggtactc ggccttggcc gcgcgctcgt cgaaatcgcc
    15121 gttgagcttg gccacgacga cggccgcgac gctgttgccc atggcgttgc agatgcacac
    15181 ggcctgggag gtgaagcggt tgatgccgaa cagcaggggc aggccttcga tgggcaggat
    15241 gcccgaggcg gtgaccgtgg cggcgaacac gatgaaagtg ccgcccgaga ccgtggcggc
    15301 gcccttcgag gtgatcagca tgatgagcag caggcccagc tgatgcgccc aggtcagcgg
    15361 cacgccgtag gcattggcga ggaacatcac gcatagcggc aggtagaccg aagtgccgtc
    15421 caggttgaac gcatagccgg tcggcagcac caggctgacc gtctgtttcg agcagcccag
    15481 cgcctggagt ttttccatca gccggggaaa ggcgctctcg gacgaaccgg tggcgaacag
    15541 gatgccgatc tccgatttga aatagcgcag gaagtgaaaa atattcatgc cgaagcagcg
    15601 caggatcacg ccgaacaaga ccaccaccac gaaggccagc atggcgtaca tgaccagcag
    15661 caggtagccc agggagagga tcacctgcat cccgctggcg gcgaccgcgt aggcgatggc
    15721 gccgaaggcg ccgatgggcg cgaagcgcat caccatgtcg gtcagtttga agaagcactc
    15781 cgacatcgtg gagaaggcgc cttccaccga ttcgcgcgtc ttggccgaga gcgccagcac
    15841 ggccgcgccg aaggcgatcg ccagcacgac cacctgcagc agcgagctgc ccgtcaacgc
    15901 gcccacgaaa ctgtcgggcg tcatggacat caggaagacg tcgaagccct gcgtggtggc
    15961 cttgccgtag gacgcgaccg ccgcggcgtg ggccgcggga tcggggtgca ggctcatgcc
    16021 gccgcccacg ccggagtaat tggccaggac catcgagatc agcagcacga tgctggagac
    16081 cacctcgaaa tagatcagag ccttcaggcc gatcttgccg actttcttca agtcgcccgc
    16141 cgacaggatg cccagcacca ggttcagaaa caccaggggc gcaaccaccg tcttgatcag
    16201 tcgcaggaaa atgtcgccca gcaccttcac cgacatgccc agccggggct cgataaagcc
    16261 cagcgccacc cccagcacga tgccggcggt gatctggaaa cccaggcggg tatagaaagg
    16321 gctggaagaa gcggccggac tggccggtat cgcggcgggc tggatagcca ttggcattgt
    16381 ctcctgttac gaaaataaat acgtactttt taatatgtac gtactttata taaggaagaa
    16441 caaggccaaa actagggtaa acacctagtt ttcaagatag aacagggctg ccagctccca
    16501 ggttggcggc ttcgcccgcc aacgtgtaga gtcctgcgtc gtaagccggc cttgcccggc
    16561 cggcaggatt accagagagc aagcaaagtc ctcatggcgt caccgcgtta cgtcgaactc
    16621 gcgcaagagc tgatccggca aattcaaagc ggccatctgg ccgtgggcgc gtccctgccc
    16681 aacgaagtgg atctatcgca gcaatacggg gtcagccgct ccaccctgcg ctcggccctg
    16741 cggatggtgc aggacctggg gctgatctcg cgccgcaagc gcgccggcat acgcgtcgag
    16801 gccgccaagc cgcgcacggc ctacgagcgc tcgctgtcca gcctggaaga cttgatgcag
    16861 tacgccaccg tgaccgagcg ccacgtgcaa agcacccagc tggtcgtttg cgacgccccg
    16921 ctcgcccgga agctggaatg cgcggtgctg caaaaatggc tgtgcgtggc catgctgcgg
    16981 gtggacatgc aaaagcccga cgagcccctg tgctggaccg acgtctatct ggacccggag
    17041 atcggcgagg ggctggggcc gcagttgctc agcagcaccg gcctgatctg cgagatgatc
    17101 gaggcccgct acggctgcaa ggtggccgag gtgcggcagg aaatccgcgc cgtcggcgtg
    17161 ccggcgcacg ccgcccaggc cctgggcgtc gcgcaggatt cgcatgcgct ggaagtcacg
    17221 cgccgctacc tcggcgaaca aggtcaccct ttcgagatca cgatcagcgt gtttcccgcg
    17281 gaccgcttcg cctacgggct ggtgctgcgg cggcatggac acggcgtcta gcctgtccgc
    17341 cgccgcgaca cgggtcaggc cgcggtctgg tccacccggt tggcttccag caccgtcagc
    17401 gccgccatat tgatgatgcg gcgcacggtg gaactggagg tcaggatgga aaccggcgcg
    17461 ttggcgccca gcaggaaggg gccgatggcc acattgccgc cggccgtggc cttgagcagg
    17521 ttgtaggcga tgttgcccga atccacgttg gggcagacca gcaggttggc cgaccccttg
    17581 agcgtggacg acggcaggat gcgcatgcgc agcgcctcgt cgagcgcgca gtcgccgtgc
    17641 atctcgccgt cgatctcgat gtcgggcgcc tgggcgcgca ccagcgccag cgcctcgcgc
    17701 atcttggcgg ccgaagccca gtcgccggtg ccgaaattcg agcgcgacag cagcgccacc
    17761 ttgggcacca gcttcaggct ggacatctgg cgggccgcgg cgatggtgaa ctcggcgatc
    17821 tgctcggccg aggggttgtc gttgacgtgc gtgtcgacca gcgcgatcgt gcgctcgttg
    17881 agcagcagga cgttcatggc cgcgtaggtc tcggcgccgg gccgcttgcc gatgacttcg
    17941 tcgacgaagc gcaggtggtc gtgataggcg cccaccgagc cgcacaccat gccgtcggcc
    18001 tcgcccaggc gcaccatcat ggcgccgatc agcgtcatgc ggcggcgcat ctcgacgcgc
    18061 gccatttcct tggtgatgcc acggcggcac atcagctccc agtaggtggt ccagtactgg
    18121 tggaagcgtt cgtcgtattc ggggttggtg acttcgacgt cctcgccgag cttcaggcgc
    18181 aggccgtatt tctcgatgcg cgacagcagc acctgcggac ggcccaccag gatgggacgc
    18241 gcgagctttt cgtcgaccac gacctgcacc gcgcgcagca cgcgctcgtc ctcgccttcg
    18301 gagaacacga tgcgggcctt gccgccttca cgcacgatgc gcttgaccgc gccgaacacc
    18361 ggcgtcatga aggcgccggt gtggtagacg aactgctgca gctgctcggc ataggcgtcg
    18421 aggtcggcga tgggccgcgt ggccacgccg tcgtccatgg ccgcgcgggc cacggccggg
    18481 gcgatgcgcg cgatcaggcg cgggtcgaag ggcttgggaa tgaaatattc gcggccgaag
    18541 cagatgtcgt aggtgccgta ggcttcggcc accacttcgt tctgctcttc ctgggccagg
    18601 ccggcgatgg cgcgcaccgc cgccttttcc atttcgcgcg tgatggtggt ggcgcccacg
    18661 tccagcgcgc cgcggaagat gtacggaaag cacagcacgt tgttgacctg gttcggatag
    18721 tccgaacggc cggtggccat gaccacgtcg tcgcgcacgg cgtgcgcgtc ttcgggccgg
    18781 atttccggcg agggattggc cagcgccagg atcagcggat tgggcgccat gaccttgacc
    18841 atctcgggct tgagcacgcc gccggccgac aggcccagga acacgtcggc gccctcgatc
    18901 acttccgcga gcttgcgctg cgcggtctct tgcgcaaagc gctccttgtc cgggtccatc
    18961 agggtggtgc ggcccttgta gaccacgcct tcgatgtcgg tggcccagat gtgctcgatg
    19021 ggcagcccca ggtcgaccag caggtccagg caggccagcg cggccgcgcc ggcgccggag
    19081 gtgacgacct tgatctcctt gatgtccttg cccacgacct tcaggccgtt gatgaaggcg
    19141 gcgcagacgg tgatggcggt gccgtgctgg tcgtcgtgga agaccgggat cttcatgcgc
    19201 tcgcgcagct tgcgctcgac ggtgaagcat tccggcgcct tgatgtcttc gaggttgatg
    19261 ccgccgaagg tgggctccag gccggcgatg atctcgacca gcttgtcggg atcggactcg
    19321 ttgatctcga tgtcgaacac gtcgatgcca gcgaacttct tgaaaagcac ggccttgcct
    19381 tccatgaccg gcttggaggc cagcgcgccg atattgccca ggcccagcac ggcggtgccg
    19441 ttggtgatca cgcccaccag gttgccgcgg ccggtgtagc ggaaggcgtc catggggttg
    19501 gcgacgattt cttcacaggc cgccgccacg ccgggcgtgt aggccagcgc caggtcgcgc
    19561 tgggtgatga gcggcttgct cgcgttgacc gacagcttcc cgggtttggg aaactcgtga
    19621 tagttgagag cggcttggcg gtttgaatta tcagtgccca tggtatgtga tcaaagtgtg
    19681 acaaaaacct gactggagcc ccatcctacg cctgaaacat aatctgatta tttggattta
    19741 atatgattgg ataagtaaat ccttatgacc atgagagcag catggctttc gattcgggcg
    19801 atgtcgccca caagctgaac aatcggctca agatgcgcca cctggccctg ctgctgcaga
    19861 tccgccagca cggctcgctc acccgcgccg ccgaacacat ggccatcagc cagccggcgc
    19921 tcaccaatac gctcgccgag ctcgaaggca tgttcggcgc gccgctgttc gagcgctcgg
    19981 cgcgcggcat ggcccccacc ctgctgggcc gggtggtcct ggcccacgcc caggccatga
    20041 tccaggacct ggaccacctg gtccgcgaca tggatgccgt ggccggcggc catacggccc
    20101 acctgcacgt gggcgtgacg cctttcgtgc cgggccgggt attgccggcc gccttgcagc
    20161 aggcggcgcg cgacggccgg cacctcaccg tcaccgtgca cgaaggcccg ggcgaactgc
    20221 tgctgccgca attgcaggac catacgctcg acatcgtggt cggccaggca tcgcccgcgc
    20281 tgaacctgga ggggctgggc ttcgacgcgc tctacagcca gtcgccccgg ctgatcgcca
    20341 accgacgcct ggccgcgcgc ctgagccgcc agcgcctgga ctggccgcgc ctggccggcc
    20401 tggactggat catgagcccg tcgaccagct acctgcgcga acaagtggcc agcctgttcg
    20461 tcgaggccgg cctgcctgcg ccgcgcccgg tggtggaaac cgacgtggcg cgcctcatcg
    20521 gcgacatgct cgcggccagc gagcgggccg tggccatcct gcccgccgat attgccgagg
    20581 agctggtgcg cgtggcgggc gtggccatca ttccctattc ctttgcctgg agcctgccgc
    20641 ccatcggttt tttcacgcgg cattccggca cgccgcgcat ggccgaacag tggtttgccg
    20701 atgcattgcg caaggccagc cattgaagcg ttgcggctga agccgccaat cgcgcgccgg
    20761 acgccggccc tggcgcccgg ccatgcggta cgctgcctgc gcgtcgacca gaccgcataa
    20821 agcgatacga tcactccttc ccctttcctg ccgcaaccaa gatgaccatc acccgagaat
    20881 ccgcccgctg cttcatgccc taccccgatg cgcccgtcga gcacgccgcg tccggcccct
    20941 tggcgggcct gagctttgcc gtgaaagacc tgttcaacgt ggccggctac cccacgggcg
    21001 gcggcaaccc gcacgtgctg gccctgtccg gcatcaaagc ggcgcacgcg gccaccgtgg
    21061 ccaagctgct tggcgcgggc gcgcgtttcg cgggcaagac tcataccgac gaactggcct
    21121 tttccatgaa cgggcagaac gcccactacg gcgcgcccgt caacggcgcc gcgccggacc
    21181 gcatcaccgg cggctccagt tccggttcgg cgtcggcggt atccaaccaa ttttgcgact
    21241 tcgccatcgg caccgacacc ggcggctcga tacgcacgcc ggccaaccac tgcaatctgt
    21301 acggcatccg ccccacccac ggccgcatca gcctggccgg cgcgctcgac ctggcgccgg
    21361 ggttcgacac ctgcggtttc ttcacgcgcg acgcggccac gttcaagaaa gtggccaacg
    21421 tattgctggg cgcggacagc gcgcccctgc ccgccaagcc ccgcctgctg ctggccgacg
    21481 acgtattcgg cctgttgtcc gcggcggtgc tgcaatcgca gcaacccgcc ttgcagaaac
    21541 tgcgtggaca actgggcgaa atgacgcccg tgcaggccgg cctgcccgac ttcgacaccc
    21601 tctattgggc cttccgccgc atccagggtt gggaagcctg gcgcgtggac ggcgcgatga
    21661 tcgaaaaata cggcctgatg ctggggtccg gcgtgaagga acgctttgcc tggtccaagg
    21721 ccgtcacgca gcagcagttc gaggaaagca gcgcgatccg cgaacagtac cgcgccatgc
    21781 tgggcaagct cctgggcagc aacggcgtgc tggtgctgcc caccatgccc ggccccgcgc
    21841 cgctttcgtc cacgcccgaa gcggaactgg acgcctatcg caacgacgcc atccgcatgc
    21901 tctgcctgtc cggcctggcg ggcttcccgc agatcagcct gcccctgggc gcgcacgatg
    21961 gcgcgccgct gggcatctcc atcctgggac cggcgggcag cgatgcgtcg ctggtggcga
    22021 tagccgccag gctggtggac ggggtcccgt tgaacgcctg atcggccatc agtctcgcag
    22081 atcaagagac tgcgaacctt gagacatcat gagcatcgtc gctggggtat aggggccggc
    22141 tgcgttgagc ggcgggcagc cccgcagggg ccgagggcgc gtcgtcttgc gcccgagccg
    22201 ccgcgatgca gccggcccct ataccccagc gacgatgcgt ctcaagaact caaatgcaca
    22261 acaaccctca ctgaaacgcc gtctccataa acgaccgcaa tttccgcgaa tgcagccgct
    22321 ccagcggcat ctcccgcaag cgctccagcg cgcggatgcc gatctccagg tgctgattca
    22381 cctgcttgcg gtagaaggcg ctggccatcc ccggcagctt caactcgccg tgcaagggtt
    22441 tgtccgacac gcatagcaag gtcccgtagg gcacccgcag ccggaaccca ttggccgcga
    22501 tcgtcgccga ctccatatcc agcgcaatgg cccgcgactg ggcaaagcgc tgcaccagct
    22561 cgctctggtt gcgcagctcc cagttgcggt cgtcgatggt ggccaccgtg ccggtgcgca
    22621 cgatgcgctt gaggtcccag cccgacaggc cggccacctc ggccacggcc tgctccagcg
    22681 ccacctgcac ctcggccagc ggcggcaccg gcacccaggt cggcaggtcg gcgtcgagca
    22741 cgtggtcgtc gcgcacatac ccgtgcgcca gcacatagtc gcccagccgc tgcgtatcgc
    22801 gcaggccggc gcaatggccc agcatcagcc aggcatgcgg gcgcagcacg gccacgtgat
    22861 cggtgatcgt cttggcgttc gacggcccca cgccgatatt gaccagcgta atgcccgaat
    22921 gccccttgcg caccaggtgg taggccggca tctgcggctg cctggcgggc gcatcgccgc
    22981 acaccgcgcc gcccagcggc gtgacgcggt tgcccggctc caccagcctc tcgtactcgc
    23041 cctgcccgct cgccatcagg ccgcgcgccc aggccaggaa ctcgtccaca tagaacgcgt
    23101 agttggtgaa cagcacgaaa ttctggaaat gcgccgggtc ggtggccgtg tagtgctgca
    23161 ggcgcagcaa tgaatagtcc acgcgcggcg cggtgaacgg cgccaagggc cgcggcccgc
    23221 cgtcgccgtc gcgccaggtg ccgttgacga tggcgtcgtc ggtcaccgcc aggtcgggca
    23281 cgtcgaacag gtcgcgcagc ggccgcctga ggttctcgat ctgctggccc tcgacattgg
    23341 cgccgtcggt gaaagcgaaa tgcagcggga tgggcagatc cgactcgccc acttccacgg
    23401 tgccgccgtg gttctgcagc agcagggaaa tctgccgctg caggtagtcg cgaaacagcc
    23461 ggggccgggt gatggtggtc tggtagcggc cgggctcggc cacgtggccg taggacagcc
    23521 ggctgtccag gatgtcgtgg ctgggcacgg tgatgcgcac ggccggatag caggcgcggg
    23581 cgcggcccgg ctgctcgcgc ccggccagcg cgtcctggaa ggcgcggcgc aggaaggccg
    23641 tgttgcgctc gtagatctcg atcagccggt ccaccgcgtc ggcggcgtcc tggaaaggct
    23701 ggaaagcgcg gaactcgggc cgggcgacag ccccggggac tgcattcatg ggcatgtttc
    23761 cctttgccgt atggggcacg gtatgcaggc gtttgagacc tgtcgaccga ttatgcctga
    23821 gcgccgtgtc cggcattttt cttttcaata aataggtttc agtctgttat tgaaatgata
    23881 tatcatagtg tttgtaagct ttcacaaccc acttccgacg cctaccgatc agacgcccgg
    23941 cccatgcaga ctcgcgactt tttccttccc atccccctcc ggtacacccc cctcaccctg
    24001 gcctgcctgc tggcctggag cccgggcgca agagcccagg accagccccc cgccgccgcc
    24061 ccggccgcca ccctggaaag cgtggtcgtg acaggcaacc ccctgggcaa cgacaccatc
    24121 gcccagccca ccacggtgct ggaaggccgc ggcctggacc tgcgccgcag cgccacgctg
    24181 ggcgagacgc tcaacggcct gcccggcgtg tcgaccacga cctacggccc catggtcggc
    24241 cggcccatca tccgcggcat ggacggcgac cgcatccgca tcctgcagaa cggcgtcggt
    24301 tcgctcgacg cctcgtcgct gtcgtacgac cacgccgtgc cgcaggatcc gctgtcggcc
    24361 gaccgcatcg agatcctgcg cggcccggcc gccctgctct atggcggcaa tgccatcggc
    24421 ggcgtggtca acaccctgga cggccgcatt ccgaccgaac ccatcaacgg cgttaccggc
    24481 gaggcgctga ccagctacgg cagcgccggc gaggaccgca acggcgccgt ccggctcgat
    24541 gccggcgacg gccagttcgc catccatgcc gacgcctatg cccggcagac cggcaacctg
    24601 cgcatacccg gctacgccca ctcggcggcg cagcgcgcca tcgactcgcc cgacgaaccc
    24661 caggcctacg gcacgctgcc caatagcgac ggcagcgacc atggcggcgc cctggggctg
    24721 gcctggaccg gcgaccacgg ctacctgggc ctgtcctata gcggccacga ctccgagtac
    24781 ggctcggtgg ccgaggaaga cgtgcgcatc cacatgcgcc aggaaaagct cggcctggcc
    24841 ggcgagctgc gcgacctgga cggctggatc aagagcgtga aaatcaccgg cgccctcacc
    24901 aattacgaac acagagaggt cgacgacggc gtcgtgggca ccacgttcaa gaaccgcggc
    24961 tacgaactcc gcatcgaagc gcgccacgcc gacatcgggc cggtatcggg cagcatcggc
    25021 ctgcagctgg ggcaaagccg gttttccgcc ctgggcgacg aagccctggt gcccaccacc
    25081 aataccgaca ccaccgccct gttcatcgtc gagcaattga aagccaccga tcgcctgacg
    25141 ctgagcgccg gcgcccgcgc cgaggacacg aacctgtcgc cctccgccgg cgccgtggaa
    25201 cgcttcgacg acagcgcgtc gcgcaatatc gtggccagca gcgcgtcgct gggcgcggtc
    25261 taccagctca atccggcctg gtcgctggcc gccaatggcg cctataccga gagggcgccg
    25321 accttctacg aactctatgc caacggcgtg cacgacgcga ccgggcaata cctggtcggc
    25381 aaccagaacc tgggcaagga gcgcgcctgg tcggccgacc tcgccctgcg ctaccagcaa
    25441 gggaccgaca aaggcagcgt gggcgtgttc tacaaccgct tcagcaacta cctggccgaa
    25501 ctcaacaccg gccgctaccg cgacgaagat ggcaatgtcg tggcgtctac cacgccggac
    25561 gccctgcccg aggcgctcta ccagggcatg ccggccgaga tatacggcgt ggaagccgaa
    25621 aaccgcaccc gtgtcctgca gcgcggcggc catacgctgg acctggacct gtcgggcgac
    25681 tatacctata cgcgcagcct gagcagcggc tcgtccctgc cccgcatacc gcccttgcgc
    25741 ctgcgcgtgg cgctggacta cgcctggggc ccctatgatg ccggcgtcgg cgtgaccaag
    25801 gccttcgccc agcaccgtca tgccgacttc gacaccccca cgcccggcta ctacagcctg
    25861 gacgtcaacc tgggctaccg gttcaagctg gggacgaccc aatggcaggc ttatgcgcgc
    25921 ggcgtcaacc tcaccaacca gaccatccgg tacgccacgt ccatcctgcg ggatatcgcc
    25981 ccggagcccg ggcgttccct attagtggga ttacggggta gtttttaatt gcccctcatg
    26041 ctacaaaagg cttaacaatc gttactggtt acaacggcga atcgcggttc atatccggta
    26101 tactctttgc tgcagcgcac ctccggccgt atgccggggt gccctgggtg atcaggtcca
    26161 gttagcttca aagttagcat cctgccgcta ttttcgccgt tctacggcag ttttttcgtc
    26221 atcaacgcct cattttaccg acgccacatg accatgccgc caacgcaaaa gcttcacata
    26281 tccctgctgg ccgccgccgc cctggctgcc agcctgctct actcccccgc ccatgccgcc
    26341 gtcgaagccg aagcgctccc ctccctgaag ggtctgagca ccgccgacca ggcaaccgtc
    26401 cccaacctgc gcgaccgcgt ggtcaagatg ggcttgaacg ccatcggcac cctctaccgc
    26461 tggggcggcg acgatcccga cgaaggtttc gactgcagcg gcctggtgcg tttcgtgttc
    26521 cgccagaccg ccggcatgac cctgccccgc accgcccgcg aacagcacac cgccggccag
    26581 tcggtggccc gccagcagct ccagcccggc gacctggtgt tcttcaacgg caccgtccgc
    26641 acccgcatcc atatcacgcg cggccaccac aagagcctgc gcacggtcgt ctccaatgtc
    26701 gtctcgcacg tcggcatcta catcggcgac aaccagttca tccacgcgcc cgtaagcggc
    26761 gaacgcgtgc gcatcgacaa cctcgatgcc tcgtactgga ccaagcacta cgccggcgcc
    26821 acccgctacc tgacgcccca ggccatcgcc cgcacggccg cggcccccgc cggcttggac
    26881 gacgataccg acgaccagcc ctgatccgtc cgcgcccagg gtaaaaacgg ccattcatcg
    26941 aatggccgtt tgcattttgc agcgctgcgg gcgtatcgct agccgcgccc gacgaatggc
    27001 atgtcggtgg ccatgatggt catgaactgc acattggtat cgagcggcag ctcggccatg
    27061 cccgccacgg cgcgcgccac gtgcgccacg tccatgcgcg gctccacctt cacgctgccg
    27121 tcggcctgga gtatgccggc cgccatgcgg tcggtcatct cggtggcggc attgccgatg
    27181 tcgatctggc cgcaggcgat atggtcgcgg cgcccgtcca gcgagatgga cttggtcagc
    27241 cccgtgaccg cgtgcttggt ggcggtgtag gccaccgaca tcggccgcgg cgcatgcgcc
    27301 gagatcgagc cgttgttgat gatgcgcccg ccgcgcggtt cctgccgctt catcaggcgg
    27361 atggcgccct gggcgcagag gaacatgccg gtgagattca cgtcgaccac ctcgcgccac
    27421 accgaagccg gcagctcgtc gatgggcacg gccggcgcgc cccggccggc attgttgaac
    27481 agcacgtcga tccgcccata agcggcttgc agctgcgcga acaaggcgtc gaccgaggct
    27541 tccttggcga cgtcggcggg caccgccagc gcattgtccg ccgccgcgcc ggccgcggcc
    27601 ttggtggcct gcagcaactc agcgcgccgg ccggccagga ccaccttgta gccgcgcgcc
    27661 aggaactcca gcgccacggc gcggccgatg ccggtgcccg cgccggtcac cagggccacc
    27721 ttgcccgcgg ggttggctgc tttcgtgtca ggggatgcca tgctcatcgt gtttctcctc
    27781 gcgcggatca tttgtcggaa tggatgcgct gcatcctagc agagccgcgc aagcggcggg
    27841 ctatactttc aggcattcca ttcatccagc cttgtcgcac gcaatggcca attctcccgg
    27901 caatatcttc atggtggtgg cccccagcgg cgcgggcaag tccagcctgg tgcgcgcgct
    27961 gctcgaccac gactcgtcca tcgagatctc gatctcgtgc accacgcgcg cgccgcgccc
    28021 cggcgaggtc gatggccgcg aataccgctt catcacgccc gaggccttcc tggccatgcg
    28081 cgatgccaat acgctgctgg aatgggccga ggtgcacggc aattactatg gcaccccgag
    28141 cgaccgcatc gacgaggcca cccgcgccgg gcgcgacgtg ctgctggaaa tcgactggca
    28201 aggcgcccgc caggtcaggc agcgctttcc gaaggctata ggcgtcttca tcctgccgcc
    28261 ctccatcgac gaactggcca agcgcctgcg cgcccgcgga caggatcccg aagaagtcat
    28321 ctcccgccgc ctgctggcgg ccggcagcga aatcgcgcat gcgccggaat gcgaatatgt
    28381 tattattaat caagaattta gcgcagccct cgcagagctg atccaaatca tcagcgttgc
    28441 gcggctgcgc tttgcgtcgc aggccgcgcg ccatgcccag ctgttcgcac agctgggcct
    28501 tccgccagag cactgacccg gccctcgccg cgtcgctgcc ctcctgccga cacccccggt
    28561 ttcccagcat ttccatccag gtatcccaca tggcccgcat cactgtcgaa gactgtctga
    28621 ccaagatccc caaccgattc aagctcacgc tggccgccac ctaccgcgcc cgcgagctcg
    28681 cgcaaggcca cgccgcgcgc atcgacagca aagacaaacc cacggtcacg gccctgcgtg
    28741 aaatcgccgc cggccaaacc ggcgcggaaa tgctgcgcaa agtcccgacc tgatctgtat
    28801 ccgggttttg ttatgacggc ggcggcatgg catttcccgg actgaaacac gcatcatcag
    28861 gcctgctggc cgcgctgcgc gcgggctccc ggctgggcct gcgatcgagc ggcggcaaag
    28921 ccgaggccgt ggccgacgcc accgagccca cctctcccgt cgcctcgctc gcgccgctga
    28981 ctgaaatcat caacggctac ctctccaagg ccgacgtcga aaaagtgcgc gaagcctacc
    29041 gcttcgccga ctccgcccac ctggggcagt tccgcgccag cggcgcgccg tacatctccc
    29101 atcccatcgc cgtcaccgag atctgcgccg gctggaagct cgacgccaac gcattgtcgg
    29161 ccgccctgct gcacgacgtc atcgaagacc agggcatcac caagccggag ctggccgaga
    29221 agttcggccc cgaagtcgcc gaactggtgg acgggctgac caagctggac cgcctggact
    29281 tcgcctccaa ggcccagcag caggccgaga gctttcgcaa gatgctgctg gccatggcgc
    29341 gcgacgtgcg cgtcatcctc atcaagctgg ccgaccgcct gcacaatatg cgcacgctcg
    29401 atgtggtcag ccccgaaaaa cgccgccgcg tggcgcgcga aacgctcgac atctacgcgc
    29461 ccatcgccca tcgcctgggc ctgaacctga tctaccgcga gttccaggac ctgtgcttcg
    29521 cggccatgta ccccaaccgc taccaggtgc tgtaccgcgc ggtgctggcg gcgcgcggca
    29581 accggcgcga ggtcatcggc aagatcgaag acgccgtgcg cgccgccctg cccgccgccg
    29641 gcatcgaggc cgaggtcacc ggccgcgaaa aaacgctcta cggcatctac cgcaagatgc
    29701 tcgcgcagaa aaagtccttt accgaagtgc tcgacatcta cggcttccgg gtgatcgtgc
    29761 acaccctgcc cgagtgctat ctggccctgg gcacgctgca ccagctctac cggccggtgc
    29821 ccggcaagtt caaggactac atcgccatac ccaagatgaa cggctaccag tcgctgcaca
    29881 ccacgctggt cggcccctac ggcacgccgg tcgagttcca gttccgcacc cgcgacatgc
    29941 accacgtggc cgaagaaggc gtggcctcgc actggctcta caaagaaagc gacacgtcgc
    30001 tcaacgacct gcaaaagcgc acgcaccaat ggctgcagtc gctgctcgac atccagagcc
    30061 agacgggcga ctcgggagaa ttcctcgagc acgtcaaggt agacctcttc cccgacgcgg
    30121 tctatgtgtt cacgccgcgc ggcaagatca tctccctgcc gcgcggcgcc acgccggtgg
    30181 actttgccta cgccatccat acggacgtcg gcaaccaggc cgtggcggcc aaggtcaacg
    30241 gcgagttcgt gccgctgcgc accgagctca acagcggcga caccatcgag atcatcacct
    30301 cgccggcctc gcgccccaat gcgcaatggc tgaactatgt gcgcaccggc cgggcccgct
    30361 ccgaaatccg ccattacctg cgcacggtca agtacgagga atcggtcaac ttcggcgagc
    30421 gcctgctgca gcagaccctc gatgagctgc acctgtccct gccgcccgcc gacgacccgc
    30481 aatgggacaa gctggcccgc agcacgggcg ccagctcgcg cgacgagatc ctgtccgaca
    30541 tcggcctggg caagcgcctg gccgccgtcg tggcgcgccg cttcgcgccc gagaactcgc
    30601 tgctggccac cacggccgcg gccatggacg agatgacttc cgcccgcgcg gcgcccatcc
    30661 tgatccaggg caacgagggg caggcggtgc aattggcgcc ttgctgcggc cccctgcccg
    30721 gcgacccgat catcgcgggg atgcggctgg gccaggggct ggtggtgcac acggcggact
    30781 gccccgtcgc gcagcgcgcc caggcgcgcg agccggaacg ctgggtgccg gtggcctggg
    30841 acagccagac aagcaagcac ttttccacca ggctggacgt cgtcacgcgc aacgagcgcg
    30901 gcgtgctggg acgggtcgcg gccgaagtcg cggcggcgga ctccaacatc atccacgtct
    30961 cgatgcacga cgacgccacg tcgacgggac tgctgcacct caccgtgcag gtggacagcc
    31021 gcacccacct ggcgcaggtg atccgggcca tccggcacgt gcctcaagtg cagaagatcg
    31081 tgcgggtcaa gggctgactc gatcgggctc ttgacccgcc cgtcgcgcag ggggcatgca
    31141 aacaagcccc ctgccgtctc catcactccc cgtacccgcc gcctcccggc gtctcgatga
    31201 cgaacacatc gccagggtgc agctgcgcgc tgtcctgcgg ccccagttcc tgcaccgagc
    31261 cgtcggcccg ctcgacgtaa ttgcgtccca cctggcccgg cgagccgccc gccagcccga
    31321 acggcgcata acggcgattg ttggacagga tcgcggccgt catgtcctcc aggaagcgga
    31381 cgcggcggat gccgccgctg ccgccctggt agcggcccgc gccacccgaa ccctcgcgga
    31441 tcgagtacga ctccaggcgc accgggaaac gcagttccag cacctccggg tcggtcagcc
    31501 gcgagttggt catgtgcgcc tggaccaccg aggtgccatg gaagcccgcc tcatgcggcc
    31561 cggtggcgtc gatgcgcgcc ggccccgcgc ccgtgccgcc cgagatcgtc tcgtaatact
    31621 ggtagcgggc attgccgaag gtgaagttgt tcatcgtgcc ctggctcgcg gcgcaggtcc
    31681 cgagcgcgcc gaacagtgca tcggtcacga cctggctggt ctcgacgttg cccgcgacca
    31741 ccgccgcctc gcgccccggg ctgagcatcg agccatcagg aatgacgagc gtcaccggcc
    31801 gcaggcagcc gtcgttcagg gggatgtcgt cgccgaccag gcagcggaag gcgtagagca
    31861 ccgccgcgcg cgtcaccgag c
//
LOCUS       DASWPO010000292         4708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1140595, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000292 DASWPO010000000
VERSION     DASWPO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140595"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VGC69_15385"
     CDS             complement(<1..440)
                     /locus_tag="VGC69_15385"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0296712.1"
                     /translation="MQTTGRKKVLIVHAHPERTSVTRRLVEISSEALTAQGHEVMQSD
                     LYAMGWKAVLDGQDFPERANQDRLSFIDESRHAFSNGCQTPDVEDEQRKILAADAVIL
                     QFPLWWFGMPAIMKGWVDRVWAYGLAYGYQDAGNAHRYGEGGFAG"
     gene            542..1447
                     /locus_tag="VGC69_15390"
     CDS             542..1447
                     /locus_tag="VGC69_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296713.1"
                     /translation="MDTQKRLDLNLLVTLETLLVEQNVTRAAERLHLSQPAVSAQLNR
                     LRELFDDPLLIPAQRGMTPTAKALDLLDPLRHALDQVRATVAGHRHFNPAKARLTVAI
                     ACTDYLQAAVVQPLAIALRKRAPGVRIALRHLDPPQLEAQMASGQVDLALMTPEHGPA
                     SLRSRHLFDERYVLIGRRRHPELRRGLSVEQFARLDQVIVSLRGGDFTTPVDEGLAAL
                     GYKRNVALSAASFLFVPDVVAHSDFIALVPERLVRDRSEALKVVECPFPVPGFAVSML
                     WHERNHSHDGQRWVREVVIELADKL"
     gene            complement(1512..2024)
                     /gene="def"
                     /locus_tag="VGC69_15395"
     CDS             complement(1512..2024)
                     /gene="def"
                     /locus_tag="VGC69_15395"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0296714.1"
                     /translation="MALLSIIRYPDARLHKKAKPVAVVDDRIRKLVRDMADTMYEAPG
                     VGLAATQVDVHERVIVIDTSEEGNNLLVLINPQITWKSEEKKVFEEGCLSVPGIYDEV
                     ERSDHIRFKALDANGQSYEREVDGLLAVCVQHEMDHLDGKVFVEYLSPLKQNRIKTKL
                     KKAERELAKA"
     gene            2288..3406
                     /gene="dprA"
                     /locus_tag="VGC69_15400"
     CDS             2288..3406
                     /gene="dprA"
                     /locus_tag="VGC69_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY0296715.1"
                     /translation="MPLTHTPDELSAWLRLSLEPELGPSLAYALLSALGEPAQIYDRR
                     ATELARHVPQDLARQLAGAPPAEISQRIEATLRWVEAPERHILTLADPGYPQQLLTIA
                     DPPLLLYVAGNPAQLSRPGLAVVGARHATPGGEENARAFARHLAMQGWSVVSGLALGI
                     DGAAHEGALAAGPDGAGTTAVMGTGIDRVYPAAHRDLAYRIAAHGALVSELALGTGVQ
                     KFHFPKRNRLVAGLARGVLVVEAAKQSGSLITARLAAESGREVFAIPGSIHSPLSRGC
                     HALIRQGAKLVETAQDITDELGSPAPAARPVPAQAPRPDTPQHPLLHALGHDPLHLDA
                     LQARTGLDTATLQAQLLELELAGHIARLDDGRFQRLAH"
     gene            3493..4401
                     /locus_tag="VGC69_15405"
     CDS             3493..4401
                     /locus_tag="VGC69_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY0296716.1"
                     /translation="MPLPTRVKLVEVSPRDGLQNEKNFIPTAVKIELIDRLAAAGFPN
                     VEAASFVSPKWVPQMSDGAEVMAGIHRRPGTIYSALTPNMKGFEGALAARADEVVIFG
                     AASEAFSQKNINCSIAESIARFEPVAQAAKAAGLRLRGSISCSLGCPYQGEVPVESVV
                     DVARRFIALGCDEVDVADTIGVGTPLLTRKVMNAVTAVVDPARVSGHFHDTYGQALAN
                     ILASLEADISIFHASASGLGGCPYAKGATGNVATEDVVYMLRGLGIETGVDFDALVDI
                     GQWISGHLDRKGGSRAGNAVAAKRAA"
     gene            4519..>4708
                     /locus_tag="VGC69_15410"
     CDS             4519..>4708
                     /locus_tag="VGC69_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEY0296717.1"
                     /translation="MTWIVLIIAGLFEVGWAVGLKYTEGFTRFWPSVGTLAAMAVSVG
                     LLGLAMRSLPVGTAYAIWT"
BASE COUNT          773 a         1633 c         1484 g          818 t
ORIGIN      
        1 cccgcaaagc cgccttcgcc gtagcgatgg gcattgccag cgtcctggta gccataggcc
       61 aaaccatagg cccacacccg gtccacccat cccttcatga tcgccggcat gccgaaccac
      121 catagcggaa actgcaggat caccgcgtcg gccgccagta tcttgcgctg ttcgtcttcg
      181 acgtcgggag tctggcaccc gttggaaaac gcgtgcctcg attcgtcaat aaaagacaga
      241 cggtcctgat tggcgcgctc gggaaaatcc tgaccgtcga gcaccgcctt ccatcccatg
      301 gcgtacaaat cggactgcat cacctcgtgg ccttgggccg tcagcgcctc ggaggatatc
      361 tcgacgagtc gacgcgtcac ggatgtccgc tcggggtggg catggacgat aagaactttc
      421 tttctgccgg ttgtctgcat ggcttggctt tcacagagca tgaaatgttg agactggttg
      481 cagtttggtt tgccctgatg tatctgtgaa atcaaaataa tttaccgcta ccatccatgg
      541 catggatacc caaaagcgct tggacctcaa tctcctggtc acgctggaaa ccctcctggt
      601 ggaacagaac gtgactcgcg cggctgagcg cctgcacctc agccagccgg cggtcagcgc
      661 gcagttgaac cgcctgcgcg aactgttcga tgaccctttg ctgataccgg cgcagcgtgg
      721 aatgacgccc actgccaagg ccctcgatct gcttgatccg cttcgccacg cgctggatca
      781 ggtgcgcgcc acggtggccg gacaccgtca cttcaacccg gcaaaggctc gactcactgt
      841 tgccatcgcc tgcaccgact acctgcaggc ggcagtggtc caaccgctgg caattgcgct
      901 gcgaaaacgg gcacccggtg tgcggattgc cttgcgccat ctcgaccctc cacagttgga
      961 agcgcagatg gccagcggcc aggtggacct ggcgctgatg acgcccgagc atggaccggc
     1021 cagtctgcgc agccggcatc tgttcgacga gcgctacgtg cttatcggca gacgtcggca
     1081 tcccgaactg cgacgaggac tttctgtgga acagttcgca cggctggacc aggtgatcgt
     1141 ctcgctgcgc ggcggcgact tcacgacccc tgtggacgaa gggctcgcag cgctaggcta
     1201 caagcgcaat gttgcgcttt ccgcggcctc gttcctgttc gtacctgatg tcgtcgccca
     1261 ctctgacttt atcgccctgg tgccggaacg gctggtgcgc gatcgcagcg aggcgctcaa
     1321 agtggtcgaa tgtcctttcc ccgtgccggg cttcgccgtg agcatgctgt ggcacgaacg
     1381 caaccatagc cacgacggac aacgctgggt acgcgaagtc gtcattgagt tggcagacaa
     1441 actgtaaccc ctgcagccga gccatggctc ttaggcgcgc agggcggccc tgggcatgcg
     1501 gcggggcggg atcaggcctt ggccagttcg cgctcggcct tcttgagctt ggtcttgatg
     1561 cgattctgct tgagcgggga caggtattcg acgaacacct tgccgtcgag gtgatccatc
     1621 tcgtgctgca cgcacacggc cagcaggccg tcgacttccc tttcgtagga ctggccgttc
     1681 gcgtcgagcg ccttgaagcg gatgtggtcg gagcgctcga cctcgtcata gatgccgggc
     1741 accgacaggc agccttcttc gaagaccttt ttctcttcgc tcttccaggt gatctggggg
     1801 ttgatcaaca ccagcaggtt gttgccctcc tcggaggtgt cgatcacgat gacgcgctcg
     1861 tgcacgtcga cctgcgtggc ggccaggccc acgccgggcg cctcgtacat ggtgtcggcc
     1921 atatcgcgca ccagcttgcg gatgcggtcg tccaccacgg cgaccggctt ggccttttta
     1981 tgcaagcggg cgtcgggata gcggatgatg gaaagcaaag ccatgacgga accggtacgg
     2041 aaagcgttga aaatgcgttg agggttggaa attcctatgg gtatatgata atcgattagc
     2101 ctattgattt ctaatagctt ttcgctaaac cacggatagc gccgcgccct gcccgggtgg
     2161 cttgcccctt gtccgccacg ccgcaaactc gtcacccccg cggccgccgg cacggcccgg
     2221 ccatgcgaca ctagcgcgct aatttcgact gtcccgcagt cttgccaccc ccattgccgg
     2281 cgcgtccatg ccgctcacgc acactcccga cgaactctcc gcctggctgc gcctgtcgct
     2341 cgagcccgaa ctgggcccgt cgctggccta tgccctgctg tccgccctgg gcgaacccgc
     2401 ccagatctac gaccgccgcg ccaccgagct ggcgcgccac gtgccgcagg acctggcccg
     2461 ccagctggcg ggggcgccgc ccgccgagat cagccaacgg atcgaggcca ccctgcgctg
     2521 ggtggaggcc cccgagcggc acatcctcac cctggccgac cccggctacc cccagcaact
     2581 gctgaccatc gccgacccgc cgctgctgct gtacgtggcc ggcaacccgg cgcagctgtc
     2641 caggcccggc ctggccgtgg tgggcgcgcg ccatgccacc cccggcggcg aggaaaacgc
     2701 ccgcgccttc gcccgccacc tggccatgca gggctggagc gtggtgagcg gcctggccct
     2761 gggcatagac ggcgccgcgc acgaaggcgc cctggccgcc ggccccgacg gcgcgggcac
     2821 caccgcggtg atgggcaccg gcatcgaccg cgtctacccg gccgcgcacc gcgacctggc
     2881 ttaccgcatc gcggcgcacg gcgcgctggt cagcgagctc gcgctgggca ccggcgtgca
     2941 gaagttccac tttcccaagc gcaaccgcct ggtggcgggc ctggcgcgcg gcgtgctggt
     3001 ggtcgaggcc gccaaacaga gcggttcgct catcaccgcc cgcctggccg cggaaagcgg
     3061 ccgcgaagtc tttgccattc caggttcgat ccactcgccc ctgtcgcgcg gctgccatgc
     3121 cctgatacgc cagggcgcca agctggtcga gaccgcgcag gacatcaccg acgaactcgg
     3181 ctcgcccgcc cccgccgcgc ggcccgtgcc ggcgcaggcc ccacgccccg acacgccgca
     3241 acacccgctg ctgcacgccc tgggccacga ccccctgcac ctggacgccc tgcaggcccg
     3301 caccggcctg gacacggcca cgctgcaggc gcaattgctg gaactggaac tggccggcca
     3361 tatcgcccgc ctggacgacg ggcggttcca gcggctggcg cactgaatac gacgcacatc
     3421 gcgtgctccc cgggtccggc cgctttgcgc tatcgttgcc acaacgcaca acgacaagac
     3481 ctggagtaga caatgcccct gcccacccgc gtcaaactcg tcgaagtctc gccgcgcgac
     3541 ggcctgcaga acgagaagaa tttcattccc acggcggtca agatcgagct gatcgaccgg
     3601 ctggccgccg ccggctttcc caatgtcgag gccgcgtcct tcgtctcgcc caagtgggtg
     3661 ccgcagatga gcgacggcgc cgaggtcatg gcgggcatcc atcgcaggcc gggcacgatc
     3721 tattcggcgc tcacgcccaa tatgaagggc ttcgagggcg ccctggccgc ccgcgccgac
     3781 gaggtggtga tcttcggcgc ggccagcgag gctttttcgc agaagaacat caactgctcg
     3841 atcgccgagt ccatcgcccg cttcgaaccc gtggcgcagg cggccaaggc ggccggcctg
     3901 cgcctgcgcg gcagcatcag ctgctcgctg ggctgcccct accagggcga agtgcccgtc
     3961 gaatcggtgg tggacgtggc ccggcgcttc atcgccctgg gctgcgacga ggtcgacgtg
     4021 gccgacacca tcggcgtggg cacgccgctg ctcacccgca aggtcatgaa cgccgtcacg
     4081 gcggtggtcg atccggcgcg cgtctcgggc cacttccacg acacctacgg gcaggcgctg
     4141 gccaacatcc tggcctcgct ggaagcggac atctcgatct tccacgcctc ggcctcgggc
     4201 ctgggcggct gtccctacgc caagggcgcc actggcaacg tggccaccga ggacgtggtc
     4261 tatatgctgc gcggcctggg catcgagacc ggcgtggact tcgacgcgct ggtcgacatc
     4321 gggcagtgga tctccggcca cctggaccgc aagggcggca gccgggccgg caatgcggtg
     4381 gcggcgaaac gggccgcctg acgcggcgat gcaagcaacg tagcgcgacg gccatcgccg
     4441 gcaaggacgg ccttgccggc tttcatgact ctcaggacga cccggacgac tggcgcatag
     4501 gcgccctgga gatgttttgt gacttggatt gttttgatca ttgccggctt gttcgaagtc
     4561 ggctgggccg tcggtctgaa gtacaccgag ggcttcaccc gtttctggcc ctccgtgggc
     4621 acgctcgccg ccatggcggt gagcgtcggg cttctcggac tggccatgcg ctccttgccc
     4681 gtgggcacgg cctatgccat ctggacag
//
LOCUS       DASWPO010000293         5762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1677823, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000293 DASWPO010000000
VERSION     DASWPO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677823"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(511..2067)
                     /gene="ubiD"
                     /locus_tag="VGC69_15415"
     CDS             complement(511..2067)
                     /gene="ubiD"
                     /locus_tag="VGC69_15415"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEY0296718.1"
                     /translation="MKYKDLRDFIAQLETRQELKRIATPVSTHLEMTEIGDRVLRAGG
                     PALLFENARHNGAAGMPVLTNLFGTPQRVAWGMGADDVSALREVGELLASLREPEAPK
                     GLRDAFAKVSMLKSALWDMSPKTLRSPACQEIVWEGQDVDLSRLPIQTCWPGDVAPLL
                     TWGLVITRGPQVGPKPARRQNLGIYRQQPIGRNKLIMRWLSHRGGALDFRDHALAHPG
                     QPFPIAVALGADPATTLGAVTPVPDSLSEYQFAGLLRGSRTETAKAIGSELSVPAWAE
                     IVLEGHLLPTGDTRAVAPVVPEGVAPPPATGYEMALEGPYGDHTGYYNEQDWFPVFTV
                     DRITMRRNPLYHSTYTGKPPDEPAVLGVALNEVFVPLLRRQLPEIVDFYLPPEGCSYR
                     LAVVSIRKQYAGHAKRVMFGLWSILRQFMYTKFIVVVDEDVNVRDWKEVVWAMTTRMD
                     PVRDTLLVENTPIDYLDFASPVSGLGGKMGMDATNKWPGETSREWGTPIRMDEAVKKR
                     VDGMWGELGL"
     gene            2294..3139
                     /locus_tag="VGC69_15420"
     CDS             2294..3139
                     /locus_tag="VGC69_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0296719.1"
                     /translation="MTDASMAGLFRRLAQGVHHQLAEWLRISSVYLGIAVIVTVSMGF
                     ALPGLRDQALQVHKALLTALAPESAMPVVADTDVAPVYGADSPTAVAMAVPDAPASTA
                     TSLSALARQQAAVRAVQSVSPVVAPSVQFESLRNYIARKYKVAYDATAVLIDTAYRVG
                     REKKLDPLLVLAVIAVESSYNPLAESAVGAQGLMQVMPRVHRDKFEDLADHGKAKPNA
                     LDPVANIIVGSGILRDCITQRGSVAGGLACYEGAAVDDAYAARVQAERRRLALASGVA
                     LARDD"
     gene            complement(3216..4373)
                     /locus_tag="VGC69_15425"
     CDS             complement(3216..4373)
                     /locus_tag="VGC69_15425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0296720.1"
                     /translation="MPQLADRANAFPPFHAVALFKETLALAAAGKRIINMGIGEPDFT
                     APPSVVDALQHAARSGLSAYSAPAGIPPLRQAIARYYDEHFGTRIDPARVIVTSGGSG
                     ALTLACAVLVDSGAEVLLPDPCYPANCNFIVAANGRPTLVPTTAASRYQLTAQDIREH
                     WGPDTRGVLIASPSNPTGTTIAPDALAELLAEVRARKGFVIMDEIYLGLTYEGQAKSA
                     LTLDDDIIVINSFSKYFNMTGWRLGWMIVPQDMVGLIERVGASLAICAPTLGQHAALA
                     CFEPEAMAIYEQRRAAFRQRRDYLVPALGKLGLEAPVKPDGAFYVYADIGRLGEDSVS
                     FSYRLLREGGVSALPGVDYGPAPHGRHTMRFSYCVGLDQLEEAVHRIGKLL"
     gene            4570..5037
                     /locus_tag="VGC69_15430"
     CDS             4570..5037
                     /locus_tag="VGC69_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEY0296721.1"
                     /translation="MTRNGQSTLLRQLRKGGAIDSEGLDKQDMVDATQELGLTLLVAD
                     CDRARSRSAVLRAIAKAVDFPEFFGGNLDALYDCLCDTVLDNKSGGIVLWLYRLHSGD
                     PVLADDASLIEGVCADTAQFASENGRVFSYVIEHAGRHPDPEPGVAAAPYGEN"
     gene            complement(5096..>5762)
                     /locus_tag="VGC69_15435"
     CDS             complement(5096..>5762)
                     /locus_tag="VGC69_15435"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY0296722.1"
                     /translation="AHHAVRVLRLRDGESIALFDGTGGEYPAVLRIEGKEAWAQLGGF
                     DPREAELPGRVTLVQGLPGSDKMDWVVEKAVELGAAALAPIRARRSVVQLSGDRAGKR
                     LAHWRRIAQAAAEQCGRNRLMAIDAPRTLEDWLAQPAAGLRLLCHPEAGATLAEALAP
                     LAGRTDADISLLVGPEGGWSEEELQAAQRAGVQAVRFGPRVLRTETAGLALIAAASAL
                     LDW"
BASE COUNT          890 a         1906 c         1992 g          974 t
ORIGIN      
        1 gctcggcgct gccctccctg atattgcgca atacgggcgg gcaccgtatc ttgcgcctcg
       61 ccgccatcgg cctggctggc tggcagctct ataaagcctg gcacgacaac cgcgagcccg
      121 aaaagaaggt cggctaggtt cagagggttc ttgggccggg ctgtccgtct gggctcttga
      181 cccgtcccgc ggttggcggg gataccccgt gagcttcgca gtgccgggct cgggcgcgcc
      241 accaggcgcc ctcggcccct gcggggctgc cccgtcactg aggcgctccc agggcatccc
      301 cgccaaccgc gggactgcat cgaggtccgt gcgcggcagt gagtttcttc tgtttttgcc
      361 gtggccgcat cagcctgtcg tgagccgcgg accccggagg aagccgaagc gcaggcgcca
      421 gtggcgccga gcatcggaat tctgcagggc agccgcggcg tctcaagaac ccagacagcc
      481 cgccccgccc aagaactcag gcagacaaag tcagagcccc aactcccccc acatcccatc
      541 cacccgcttc ttcaccgcct catccatccg gataggcgta ccccattccc ggctggtctc
      601 gcccggccac ttgttggtgg cgtccatccc catcttgccc cccagtcccg acaccggcga
      661 ggcgaagtcc agatagtcga tgggcgtgtt ctccactaat aaggtatcgc gcaccggatc
      721 catgcgggtc gtcatggccc agaccacttc cttccagtcg cgcacgttca cgtcctcgtc
      781 caccaccacg atgaacttgg tgtacatgaa ctgccgcagg atgctccaca gcccgaacat
      841 cacccgcttg gcgtggccgg cgtactgctt gcggatcgac accaccgcca ggcggtagct
      901 gcagccctcg ggcggcaggt aaaaatcaac gatttcaggt agttgccggc gcaagagcgg
      961 cacgaacact tcgttcaacg ccacgcccag cacggccggc tcgtcgggcg gcttgccggt
     1021 gtaggtggaa tggtagaggg gattgcgccg catggtgatg cggtccaccg tgaacaccgg
     1081 gaaccagtcc tgctcgttgt aatagccggt gtggtcgccg taggggcctt ccagcgccat
     1141 ctcgtagccg gtggcaggcg gcggcgccac gccttcgggc acgacgggcg ccacggcgcg
     1201 cgtgtcgccg gtcggcagca ggtggccttc gagcacgatc tcggcccagg cgggcaccga
     1261 cagctcgctg ccgatggcct tggccgtctc ggtgcgcgag ccgcgcagca ggccggcgaa
     1321 ctggtattcg gacaggctgt cgggcaccgg cgtgacggcg cccagggtgg tggccgggtc
     1381 ggcgcccagg gcgacggcga tgggaaaggg ctggcccggg tgcgccaggg catggtcgcg
     1441 gaagtccagc gcgccgccgc ggtgcgagag ccagcgcatg atgagcttgt tgcggccgat
     1501 gggctgctgc cgatagatgc ccaggttctg ccggcgggcg ggtttgggtc cgacctgggg
     1561 gccgcgggtg atcaccaggc cccaggtgag caggggggcg acatcgcccg gccagcaggt
     1621 ctggatgggc aggcgcgaca gatcgacgtc ctggccttcc cagacgattt cctggcaggc
     1681 cgggctgcgg agggtcttgg gactcatgtc ccacagggcc gacttgagca tcgacacctt
     1741 ggcgaacgcg tcgcgcaagc ccttgggcgc ttcgggttcg cgcagcgagg cgagcagctc
     1801 gccgacctcg cgcaaggcgc tgacgtcgtc ggcgcccatg ccccaggcca cgcgctgggg
     1861 cgtgccgaac agattggtca gcaccggcat gccggcggcg ccgttgtggc gcgcgttctc
     1921 gaacagcagg gccgggccgc cggcgcgcag gacgcggtcg ccgatctcgg tcatttccag
     1981 gtgggtggat acgggggtgg cgatacgctt gagttcttgc cgggtttcaa gctgggcgat
     2041 gaaatcgcgc aaatctttgt atttcacaaa tgccttgcaa atcaggggct tgtctatgcc
     2101 tgtccaggca ggctggagga tttttcagtt tagcagtgtt gtttctggat gtgagtttca
     2161 aatcgtgctt gtcagacgag gcagactggg ttaggcgaag cacttcagta caaattccgg
     2221 catattttgt tacatttaac cccatatctt gcggttaaga tgcggcctca ttcacttcta
     2281 cgcaggaggt ctcatgactg atgcgtcaat ggccggtctc ttccgacgcc tggcccaagg
     2341 agtgcaccac cagctggccg aatggctgcg cataagttcc gtctatctcg gcatagcggt
     2401 cattgtgacg gtcagcatgg gatttgcctt gcccggtcta cgcgaccagg cgctacaggt
     2461 gcacaaagcc ttgttgactg ctcttgcccc cgaatccgcc atgccggtcg tggcggatac
     2521 cgatgtcgct cccgtctacg gcgccgatag ccccacggcc gtggccatgg ccgtgcccga
     2581 tgcgccggcc agcaccgcca ccagcctgtc ggccctggcc cgccagcagg cggccgtccg
     2641 tgcggtccaa tcggtttcgc ccgtcgttgc cccgagcgtg cagtttgaat cgctgcgcaa
     2701 ctatatcgcc cgcaagtaca aggtggccta tgacgccacc gcggtgctga tcgatacggc
     2761 ctaccgcgtg ggccgcgaga aaaagctcga cccgctgctg gtgctggcgg tgatcgccgt
     2821 cgagtccagc tacaacccgc tggccgagag cgccgtgggc gcccagggcc tcatgcaggt
     2881 gatgcctcgc gtgcaccggg acaagttcga ggacctggcc gaccatggca aggccaagcc
     2941 caatgcgctg gatccggtgg ccaacatcat cgtgggctcg ggcattttgc gcgactgcat
     3001 cacccagcgc ggttccgtgg cgggcggcct ggcctgctac gaaggcgccg cggtggacga
     3061 cgcctatgcc gcccgtgtcc aggccgagcg ccgccgcctg gcgctggcct cgggcgtggc
     3121 cctggcccgc gacgactgat ccgcgcgccg gatggcgtga aaagggcggc ccgtaaggcc
     3181 gccctttcgc atgctcgatg atcgggcttg cgcccctata gcaatttgcc gatgcggtgg
     3241 acggcctctt ccagctggtc caggccgacg caataggaaa accgcatggt gtgccgcccg
     3301 tgcggggccg ggccgtagtc gacgcccggc agggccgaga cgccgccctc gcgcagcagc
     3361 cggtatgaaa agctcacgct gtcctcgccc agcctgccga tgtccgcata gacatagaag
     3421 gcgccgtcgg gcttgaccgg cgcctccagc ccgagttttc cgagggcggg caccagatag
     3481 tcgcgccgct ggcggaaggc tgcgcgccgc tgttcgtaga tggccatggc ctcgggctcg
     3541 aagcaggcca gggcggcatg ctggcccagg gtgggcgcgc agatggccag gctggcgccg
     3601 acccgctcga tcaggcccac catgtcctgc ggcacgatca tccagcccag gcgccagccc
     3661 gtcatgttga agtacttgga gaagctgttg atgacgatga tgtcgtcgtc cagcgtcagg
     3721 gccgatttcg cctggccctc gtaggtgagg cccaggtaga tctcgtccat gatgacgaag
     3781 cccttgcggg cgcgcacctc ggccagcagc tcggccaagg cgtcgggggc gatcgtggtg
     3841 ccggtggggt tgctgggcga ggcgatcagc acgccgcggg tgtcggggcc ccaatgctcg
     3901 cggatgtcct gcgccgtcag ctggtagcgg ctggcggccg tggtggggac gagtgtcggg
     3961 cggccgttgg cggccacgat gaaattgcag ttggccggat agcacgggtc gggcagcagc
     4021 acctcggcgc cgctgtcgac cagcacggcg caggccagcg tcagggcgcc ggagccgccg
     4081 gacgtgacga tgacgcgggc cgggtcgatc cgggtgccga agtgctcgtc gtaatagcgg
     4141 gcgatggcct ggcgcaatgg cgggatgcca gccggcgcgc tataggcgct caggccggaa
     4201 cgggcggcat gctgcagcgc gtcgaccacg ctgggcggcg cggtgaagtc cggctcgccg
     4261 atgcccatat tgatgatgcg cttgccggcc gcggccaggg ccagggtttc cttgaacaat
     4321 gcaacggcat gaaacggcgg aaaagcgttg gcgcggtcgg caagctgggg catggaaaaa
     4381 aacaatcggc aaggctaaaa acacattgta gtcatgggtt gcagccggtc tgtctggttc
     4441 ccgctgcaat gagcgcaaag tgtcgcgcgg cacatacctt tttttgcgga ctcggtgtat
     4501 cgtccgtgtg ccgctgaggt agcggcgcct agcgatcagg tcacccggtt tttgcgagga
     4561 ggcacgggca tgactcggaa tggtcaatct accctattgc ggcagctgcg caagggcggc
     4621 gccatcgaca gcgagggcct cgacaagcag gacatggtcg atgccacgca ggaattgggc
     4681 ctgaccctgc tggtggccga ttgcgatcgc gcgcgcagcc gctccgcggt gttgcgcgcc
     4741 attgccaagg ccgtggattt tcccgagttc ttcggcggca atctggatgc cctctacgat
     4801 tgcctgtgcg ataccgtgct cgacaacaag tcgggcggca tcgtgctgtg gctgtaccgg
     4861 ctgcattccg gcgatccggt gttggccgac gatgccagcc tgatcgaagg cgtatgcgcc
     4921 gatacggctc agtttgccag cgagaacggc cgcgtgtttt cctatgtgat cgagcacgcc
     4981 ggccggcatc ccgacccgga acccggcgtg gcggcggcgc cgtacggcga gaactaggca
     5041 ggggcgggcg gcccgcgggg gtgccatccc ttgggctgct accggtcttc cgggcttacc
     5101 aatccagcag ggcgcttgcc gccgcgatca aggccaggcc cgccgtctcg gtgcgcagca
     5161 cccgcgggcc gaagcgcacg gcctgcacgc cggcgcgttg cgcggcctgc agctcctcct
     5221 ccgaccatcc cccctcgggt ccgaccagca ggctgatgtc ggcgtccgtc cgtccggcca
     5281 gcggcgcgag cgcctcggcc agcgtggcgc cggcctcggg gtggcacagc aggcgcaggc
     5341 cggcggcggg ctgcgccagc cagtcttcca gggtgcgggg ggcgtcgatg gccatcagcc
     5401 ggttgcggcc gcactgctcg gccgccgcct gggcgatgcg ccgccagtgc gccaggcgct
     5461 tgccggcccg gtcaccggaa agctgcacca cgctgcgccg ggcccggatg ggggccaggg
     5521 cggcggcgcc gagctccacg gccttctcca ccacccagtc catcttgtcg ctgcccggca
     5581 ggccctgcac cagcgtgacg cgccccggca gctcggcttc gcgcgggtcg aagccgccca
     5641 attgggccca ggcctccttg ccttcgatcc gcagcacggc cgggtattcg ccgcccgtgc
     5701 cgtcgaacag cgcgatggat tcgccgtcgc gcagccgcag cacgcgcacg gcatggtgcg
     5761 cc
//
LOCUS       DASWPO010000294         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_781903, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000294 DASWPO010000000
VERSION     DASWPO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781903"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..513)
                     /locus_tag="VGC69_15440"
     CDS             complement(94..513)
                     /locus_tag="VGC69_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HEY0296723.1"
                     /translation="MRDLIAAVSIAGLLIPEAVAAVLAVLIVRPKAQLFFANADKLLN
                     GVRQLIQQAQPPVRAVMLSREESPDLDGTALEALQTFAAECRARNQRLVLARLKPDAL
                     HVLHALSDGGCAGAVLSELSVDESLQPLLAQVSTSAA"
     gene            complement(513..1139)
                     /locus_tag="VGC69_15445"
     CDS             complement(513..1139)
                     /locus_tag="VGC69_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HEY0296724.1"
                     /translation="MAYGNDVHLISARLDRLRPTQMTVGYREVQAKRKHWQTLGKKER
                     RSTLETHWFPAVLGPGGEYFIVDHHHLGLALIEEDVADARIMLLKDLSWLDMTIFWRM
                     MEHNLWVHPYDVDGSRRDYARLPKTLTRLKDDPYRSLAGEMRTSGGYAKEATPFSEFL
                     WADYLRPRITPAAVRKNFSKALDMALALAHEQDARYLPGWSGIVEAKG"
     gene            1514..>2009
                     /locus_tag="VGC69_15450"
     CDS             1514..>2009
                     /locus_tag="VGC69_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0296725.1"
                     /translation="MSGIALAFSQAAVADVTVKIGSAAPLTGGIAHIGQDQADGVKLA
                     IDEINKTGLTINGEKVTLEFDSQDDAADPKTGTQVAQKLVDDGVVAVVGHLNSGVSIP
                     ASKIYAAAGVAQITPASTNPNYTLQGLKTTFRVIGTDAQQGPTLAKYTKEMKAKSVAI
                     VDDAT"
BASE COUNT          396 a          670 c          594 g          349 t
ORIGIN      
        1 tcggcagtct tcaatcggga ggcagcggcc tggcctggct ctcggaactt gccccggccg
       61 cctgccaacc aggcagcgag gggcttttcg tttttacgct gcagacgtgg aaacctgcgc
      121 caataacggc tgcaggcttt cgtccacgct aagttcggac agcaccgctc ccgcgcagcc
      181 gccgtccgac aacgcgtgca ggacatgcag cgcatccggc ttcaggcgcg ccagcacgag
      241 ccgctgattg cgcgcccggc attcggcggc gaaggtctgc agggcctcga gcgccgtgcc
      301 atcgaggtcg gggctttcct cccggctgag catgacggcc cggaccggcg gctgcgcctg
      361 ctgaatcagc tgccgcacgc cattgagcag tttgtcggcg ttggcgaaga acagctgcgc
      421 cttgggccgc acgatgagca cggccagcac cgccgccacg gcctcgggaa tgagcaggcc
      481 ggcgatcgat acggccgcga tgagatcgcg catcagccct tggcctcgac gatgccggac
      541 cagcctggca gatagcgcgc gtcctgctcg tgcgccagcg ccagcgccat atccagcgcc
      601 ttggagaaat tcttgcggac ggccgccggc gtgatgcgcg ggcgcaggta gtcggcccac
      661 aagaactcgc tgaacggcgt ggcttccttg gcatagccgc ccgaggtgcg catctcgccc
      721 gccaggctgc gatagggatc gtccttgagc cgggtcaggg tcttgggcaa gcgggcgtaa
      781 tcacggcgcg agccatcgac atcgtaggga tgcacccata ggttgtgttc catcatccgc
      841 cagaagatcg tcatgtcgag ccaggaaaga tccttgagca gcatgatccg cgcatcggcc
      901 acgtcttctt cgatcaaggc caggcccagg tggtggtgat cgacgatgaa gtactcgccg
      961 cccgggccca gcacggccgg gaaccaatgc gtctcgagcg tgctgcggcg ctctttcttg
     1021 cccagcgtct gccagtgctt gcgcttggcc tggacctcgc ggtaacccac cgtcatctgc
     1081 gtgggccgca ggcgatcgag ccgcgccgag atcaaatgca cgtcgttgcc gtatgccatg
     1141 tgctgggttc ccttgcgaat tccgcgcgaa gtgcggcgcg gcaagatcgt agtcctatcc
     1201 aggcctggaa tagcgttccc gccatccccc ggaatagcga acgaatattg ccggccgctt
     1261 tcccgcttgc tgacctgcag ttgaaatatt tgtcggcatt ttcctaggga ctttccccta
     1321 taagaaaaaa ccctaataaa ttcaaatatt taccggaaca gccattacta ttgcgaattg
     1381 gaacacatgc gaaaatctcg ccactgtcga taccctcggc agaagaccct gtcggcatgt
     1441 tttaactgca acccaattca aggggccaaa gcatatgaag tttcaactca aatccaccag
     1501 caccgttctg atcatgtccg gtatcgccct ggccttcagc caggccgctg tcgccgacgt
     1561 caccgtgaag atcggcagtg ccgccccgct gactggcggt atcgcccaca tcggccagga
     1621 tcaggccgac ggcgtcaaac tggccatcga cgaaatcaac aagacaggcc tgaccatcaa
     1681 cggcgaaaag gtcacgctgg aattcgactc ccaggacgac gccgccgatc cgaagaccgg
     1741 cacgcaggtc gcccagaagc tggtcgacga cggcgtggtc gccgtggtgg gccacctgaa
     1801 ctcgggcgtg tccatccccg cctcgaagat ctatgccgcc gccggcgtcg cccagatcac
     1861 cccggcctcg accaacccca actacacgct gcaaggcctg aagaccacct tccgcgtgat
     1921 cggcaccgac gcgcagcaag gccccaccct ggccaagtac accaaggaaa tgaaggccaa
     1981 gagcgtcgcc atcgtcgatg acgccaccc
//
LOCUS       DASWPO010000295        11125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_962878, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000295 DASWPO010000000
VERSION     DASWPO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962878"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /locus_tag="VGC69_15455"
     CDS             complement(<1..2139)
                     /locus_tag="VGC69_15455"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY0296726.1"
                     /translation="MTTPRINQHGLQVAKSLYDFIEQEALPGTGLDSATFWKGFVALA
                     REMAPRNRELLTRRDQFQQELDNWHRAHPGPITDPSGYRAFLESIGYLQPAPAVVKVH
                     TANVDTEITLQAGPQLVVPVSNARYAINAANARWGSLYDALYGTDAIDQGGDMAPTGD
                     YNPKRGQAVIARARAFLDQAAPLAEGSHADATGYAVRDGKLVVATPQGGTGLQSPEKF
                     AGYQGDAAQPSAILLCNNGLYFEIQVDRGHAIGKTDAAGVKDVLVEAAITTIMDCEDS
                     VAAVDADDKVHIYRNWLGLMKGDLAEAVTKNGKTFTRRMNPDRRYTAPQGGSLTLHGR
                     SLMLVRNVGHLMTTPAVVNEEGRELPEGILDAVLTTLGALPDLKNKLNSRTGSIYIVK
                     PKMHGPAEVLFASELFTRVEDLLDLPRNTLKMGIMDEERRTSVNLKACIQAASERVVF
                     INTGFLDRTGDEMHTAMEAGPMMRKGDMKSSIWISAYERNNVLVGLDCGLRGHAQIGK
                     GMWAMPDLMAAMLEQKIAQPKAGANTAWVPSPTAAALHALHYHMVDVQAVQQELEKTP
                     YASVRDQLLEGLLTVPVVAAPQWKPEQIRAEIENNAQGILGYVVRWIDQGVGCSKVPD
                     IHDIGLMEDRATLRISSQHIANWLRHGIADRAQVEETFQRMAAVVDRQNAADPHYRPM
                     APDFQASFAYRAACALVFEGLSQPNGYTEPL"
     gene            2422..3186
                     /gene="glcC"
                     /locus_tag="VGC69_15460"
     CDS             2422..3186
                     /gene="glcC"
                     /locus_tag="VGC69_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HEY0296727.1"
                     /translation="MDRQVEPPARLVADVVAERVEGLIADGVLKSGQLLPSERRLTEK
                     LGVSRTALREGLRLLRARGIIHTEQGKGSYVAQIAQVDAGPLVHLFHSQPRTLYDLLE
                     VRALLEGESARLAALRGTEADFIMIRRRYEAMLAAHDQGADTTRHAHLDHAFHLSVCE
                     ASHNPVLVHTLQALNDLMLGSVFACVNNLYHREPHKREIDRQHADLFDAVINRQPGPA
                     HRAATAHVDSVRQNLSDIEQEEQRLVRATRRLEGWA"
     gene            complement(3202..3393)
                     /locus_tag="VGC69_15465"
     CDS             complement(3202..3393)
                     /locus_tag="VGC69_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296728.1"
                     /translation="MLYLSWRVTEDADLRGKAGRLYSVFDAGLVTGNLPGTRIVFSED
                     AINQSSGKRVHRVVAERAG"
     gene            complement(3404..4327)
                     /gene="ubiA"
                     /locus_tag="VGC69_15470"
     CDS             complement(3404..4327)
                     /gene="ubiA"
                     /locus_tag="VGC69_15470"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY0296729.1"
                     /translation="MTSTTSSSPDLNDIVFDDWVERWLPKPWRPYARLCRLDRPIGTW
                     LTLLPCIAALVQSADGWPTLLRLIVFSLGALLMRGIGCTVNDMWDRDIDKHVERTRFR
                     PLTSGQLSMKQAVAFLVGQLAACASLLFFINGFSRWLALAVLPFVFIYPFCKRVTYWP
                     QAVLGICFNWGMLMAWSDTRSVLPLASVAMWLGAVLWQIGYDSLYAYVDVRDDLRLNL
                     RSTAMRFADRGKQWIGGFYLATLLLWTWAGYSLDAGWFYYLGMIALAVHFGWQMAVFD
                     LQKPDRNFKLFRANLWVGVILIVAALGGALL"
     gene            complement(4358..5557)
                     /locus_tag="VGC69_15475"
                     /pseudo
     CDS             complement(4358..5557)
                     /locus_tag="VGC69_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927291.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            5748..7370
                     /locus_tag="VGC69_15480"
     CDS             5748..7370
                     /locus_tag="VGC69_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0296730.1"
                     /translation="MTASPVSVPEYDYIIVGAGTAGCLLANRLSADPAARVLLLEAGG
                     QDDWHWIHIPVGYLYCIGNPRTDWCYRTQADPGIAGRSLGYPRGRVLGGSSSINGMIY
                     MRGQRADYDGWAAQGNAGWGWDDVLPLFKAVEDHHAGANAFHGAGGEWRVERQRLAWS
                     LLDAFRAAAAQAGIAPVNDFNQGDNEGCDYFEVNQRRGVRWNASKAFLRPALRRPNLQ
                     VITGAQVAGLSFEGRRARGVRYRLADGVEREARAVGEVALAAGAIGSAQLLQVSGIGP
                     QALLCSLGVPVLHELPGVGGNLQDHLQLRLVYQVSNARTLNTMANSLWGKAAMGLQYA
                     LARRGPLAMAPSQLGAFARSGPEQARANVQYHVQPLSLEKFGEPLHGFPAFTASVCNL
                     QPKSRGHVRIASADARTAPEILCNYLSTDEDCKVAADSIRLTRRIVSQPALAPYRPEE
                     RKPGATLQSEAELIAAAREIGTTIFHPVGTCKMGHDPLAVVDDQLRVHGIEGLRVIDA
                     SIMPTITSGNTNSPTLMIAEKGARLLRQARGG"
     gene            7532..8806
                     /locus_tag="VGC69_15485"
     CDS             7532..8806
                     /locus_tag="VGC69_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HEY0296731.1"
                     /translation="MPHTIEIAATLCFAVAVLHTFSVPFFARLAHRGGPHAGAWHLLA
                     EVEVVFGVWAFILMVVMALLANVRDATHYLDTRNFTEPLFVFAIMVVAASRPVLELVR
                     LLLRGALRLLPVRRELGLFWLVMALVPLAGSFITEPAAMTLAAILLREGYLRAPGHTG
                     FKYLALGVLFVNVSIGGVLTAYAAPPVLMVATTFGWDTAYMFRHFGDRAVVAVLVNAL
                     GLVLVCRTAILDSALQSAGQGEGKAGGRAPVPPLVIAVHLLFLVGVVLAAHHPAVFLG
                     LLMLFIGFCRAYERHQDRLLIKEGLLVGFFLAGLVVLGGLQQWWLQELLGSLPPLVLY
                     WGAMALTALTDNAALTYLGSLVQGTSEAWRVMLVAGAVTGGGLTVIANAPNPAGYALL
                     KGAFPDGAVSAGRLFLAALAPTLVAAAAFLLI"
     gene            complement(8803..9126)
                     /locus_tag="VGC69_15490"
     CDS             complement(8803..9126)
                     /locus_tag="VGC69_15490"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0296732.1"
                     /translation="MTVFHVSEAQADLAALQDYMLAKWGSALCDQALEEVYDQMDALD
                     QGKLNGVPVVELIELGIDGYLMTQTSHHRIIYKQVGGNTYVYIVAGQRQDFLQVLETR
                     LMQMR"
     gene            complement(9126..9404)
                     /locus_tag="VGC69_15495"
     CDS             complement(9126..9404)
                     /locus_tag="VGC69_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY0296733.1"
                     /translation="MKLSVQIKPISYLRTHAADIAKGFAEDPAPYIITHNGEAKMVVM
                     DIKTYEQDQERLALLKLVAMGEKSRLEGKVLTAEESRARLAKRRKGMR"
     gene            9607..9909
                     /locus_tag="VGC69_15500"
     CDS             9607..9909
                     /locus_tag="VGC69_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY0296734.1"
                     /translation="MPIYAYKCSACGHAEDVLQKISDAPLTVCPSCGQATYAKQVTAA
                     GFQLKGSGWYVTDFRGNGGSGGASPPAKPAAASGDSGGSAAPAAPASPTTPSSSST"
     gene            9925..10584
                     /locus_tag="VGC69_15505"
     CDS             9925..10584
                     /locus_tag="VGC69_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY0296735.1"
                     /translation="MFKKFFITGLLVWVPLAITLWVISLVVTTLEAFVPGFLSSEALF
                     GMQIPGFGFVLVIVVILLTGVFTANFLGRSLLVQWERLLSHIPLVRSIYNSVKQVSDT
                     VLAPNGRAFRQAVLIEYPRKGSWTIAFLTGEPGGEVAERLPGEHLSVYVPTTPNPTSG
                     FFLIMPRSEVIELQMTVDAALKYIVSMGVVAPAVHEAPEALPEASDARIVAQAAPPAE
                     S"
     gene            10618..>11125
                     /locus_tag="VGC69_15510"
     CDS             10618..>11125
                     /locus_tag="VGC69_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY0296736.1"
                     /translation="MSRTCYTGHVCRDHLGQTVTLYGWVNRRRDHGGVIFIDMRDRAG
                     LAQIVFDPDNAEAFKIAESLRNEFCIRITGLVRERPEGTTNPELASGEVEILCREVEI
                     LNASVTPPFQLDDDNLSETTRLTHRVLDLRRSQMQRNLMLRYRVSIEVRKYLDELGFI
                     DIETPMLTK"
BASE COUNT         1689 a         3709 c         3748 g         1979 t
ORIGIN      
        1 cagcggctcg gtgtagccgt tgggctggct caggccctcg aacaccagcg cgcaggccgc
       61 ccggtaggcg aacgaggcct ggaaatccgg cgccatcggc cggtagtgcg ggtcggccgc
      121 gttctgccgg tcgaccaccg cggccatgcg ctggaaggtt tcctcgacct gggcgcgatc
      181 ggcgatgccg tggcgcagcc agttggcgat gtgctggctg gagatgcgca gcgtggcgcg
      241 gtcttccatc aggccgatgt cgtggatgtc gggcaccttg gagcagccca cgccctggtc
      301 gatccagcgc accacatagc ccaggatgcc ctgggcattg ttctcgatct cggcgcggat
      361 ctgctcgggc ttccattgcg gcgcggccac cacgggcacg gtcagcaggc cttccagcag
      421 ctggtcgcgc acgctggcgt acggggtctt ttccagttcc tgctgcacgg cctgcacgtc
      481 gaccatgtgg tagtgcaggg catgcagcgc ggccgcggtg ggcgagggca cccaggcggt
      541 gttggcgccc gccttgggct gggcgatctt ctgctccagc atggcggcca tcaggtcggg
      601 catggcccac atgcccttgc cgatctgcgc atggccgcgc aggccgcagt ccaggcccac
      661 cagcacgttg ttgcgctcgt aggcggagat ccagatgctg gacttcatgt cgcccttgcg
      721 catcatgggg ccggcttcca tggccgtgtg catctcgtcg ccggtgcggt ccaggaagcc
      781 cgtgttgatg aacaccaccc gctcgctggc cgcctggatg caggccttga ggttgacgct
      841 ggtgcggcgc tcctcgtcca tgatgcccat cttgagcgta ttgcgcggca ggtccagcag
      901 gtcttcgacg cgcgtgaaca gctcgctggc gaacagcacc tcggccgggc cgtgcatctt
      961 gggcttgacg atgtagatgg accccgtgcg ggagttgagc ttgttcttga ggtcgggcag
     1021 ggcgcccagc gtggtgagca cggcgtcgag gatgccctcg ggcagttcgc ggccttcttc
     1081 gttgaccacg gccggcgtgg tcatcaggtg ccccacattg cgcaccagca tcagcgagcg
     1141 gccgtgcagg gtcaggctgc cgccctgggg cgcggtgtag cggcggtcgg ggttcatgcg
     1201 gcgggtgaag gtcttgccgt tcttggtgac cgcctcggcc aggtcgccct tcatcaggcc
     1261 cagccagttg cggtagatgt gcaccttgtc gtcggcatcg acggcggcca ccgagtcttc
     1321 gcagtccatg atggtggtga tggcggcctc gaccagcacg tccttcacgc cggcggcgtc
     1381 ggtcttgccg atggcgtggc cgcggtcgac ctggatctcg aagtacaggc cgttgttgca
     1441 cagcaaaatg gccgagggct gggcggcatc gccctggtag ccggcgaact tctcgggcga
     1501 ctgcaggccg gtcccgcctt gcggggtggc gaccaccagc ttgccgtcgc ggaccgcgta
     1561 gccggtggca tcggcatgcg aaccctcggc cagcggcgcg gcctggtcga ggaaggcgcg
     1621 cgcccgggcg atgacggcct ggccgcgctt ggggttgtag tcgccggtgg gcgccatgtc
     1681 gccgccctgg tcgatggcgt cggtgccgta gagcgcgtcg tacaggctgc cccagcgcgc
     1741 gttggcggcg ttgatcgcgt agcgcgcgtt ggagacgggc accaccagct gcggcccggc
     1801 ctgcagggtg atttccgtgt cgacgttggc cgtatggacc ttcaccacgg ccggcgcggg
     1861 ctgcaggtag ccgatgcttt cgagaaaggc gcggtagccc gacgggtcgg tgatcgggcc
     1921 gggatgggcg cggtgccagt tgtcgagctc ttgctggaac tggtcgcggc gggtgagcag
     1981 ttcgcggttg cgcggcgcca tttcgcgggc cagggccacg aagcctttcc agaacgtggc
     2041 gctgtccagg ccggtgccgg gcagggcctc ctgctcgatg aagtcgtaca gggatttggc
     2101 cacctgcagg ccgtgttgat tgatgcgagg ggtcgtcatg cgcgtctcga tggtggattg
     2161 ttggtatggt gccgccgcgc tttttttatc actgtgcggt gtcaccgaga tgatggccgc
     2221 gcggggcggg gcttgtccat atcaaaacca ctggtcatac cagtttttct atcttgcact
     2281 ttggggtgct ggaatttttt cctttatatt tcattgggtt gataaaaaac cctgcgttgg
     2341 gccgggcatg gccaaaaatt ggtcatacca gtcccgcgca cagccggccc gtattcccgg
     2401 gcgcggtttt caggaaaagc catggatagg caagtcgaac cgcccgcccg cttggtcgcc
     2461 gacgtcgtcg ccgagcgcgt cgagggcctg atcgccgacg gggtgctcaa gagcgggcag
     2521 ctcctgcctt cggagcgccg cctcaccgaa aagctgggcg tgtcgcgcac ggcgctgcgc
     2581 gagggcctgc gcctcttgcg cgcgcgcggc atcatccata ccgagcaggg caagggctcc
     2641 tacgtggcgc agatcgcgca ggtcgatgcc gggccgctgg tgcatttgtt ccattcgcag
     2701 ccccgcacgc tgtacgacct gctcgaggtg cgcgccctgc tcgaaggcga gtccgcccgc
     2761 ctggccgccc tgcgcggcac cgaggccgac ttcatcatga tccgccgccg ctacgaggcc
     2821 atgctggccg cgcacgacca gggggcggac acgaccaggc acgcccacct cgaccatgct
     2881 ttccacttgt cggtatgcga ggcctcgcac aacccggtgc tggtgcacac cctgcaggcg
     2941 ctcaatgacc tgatgctggg ttcggtgttc gcctgcgtca acaacctcta ccaccgcgag
     3001 ccgcacaagc gcgagatcga caggcagcac gccgatctgt tcgatgccgt catcaaccgc
     3061 cagcccggcc cggcgcatcg cgccgcgacc gcgcacgtgg acagcgtgcg gcagaacttg
     3121 agcgacatcg agcaggaaga gcagcgcctg gtgcgggcga cgcggcggct ggaggggtgg
     3181 gcctaggcgg ggggcgggcg cttacccggc ccgctcggcc acgacgcgat ggacccgctt
     3241 gccggaggat tggttgatgg cgtcttcgct gaacacgatg cgggtgcctg gcagattgcc
     3301 cgtgaccagt ccggcatcga agacggaata cagcctgccg gccttgcccc gcaggtccgc
     3361 gtcttcggtc acgcgccagc tgaggtagag cacgccgccc ggcttacagc aaggcgccgc
     3421 ccagcgccgc cacgatcagg atcacgccca cccacagatt ggcgcggaac agcttgaaat
     3481 tacggtccgg cttttgcagg tcgaacacgg ccatctgcca gccgaaatgc acggccagcg
     3541 cgatcatgcc caggtaatag aaccagcctg catccaggct gtacccggcc caggtccaca
     3601 gcagcagggt ggcgaggtag aagccgccta tccattgctt gccgcggtcg gcaaagcgca
     3661 tggccgtgga gcgcaggttc aggcgcaggt cgtcgcgcac gtccacatag gcatagaggc
     3721 tgtcatagcc tatctgccac agcaccgcgc ccagccacat ggccacgctg gccaggggca
     3781 gcacgctgcg cgtgtccgac caggccatca gcatgcccca gttgaagcag atgcccagca
     3841 cggcctgcgg ccagtaggtg acgcgcttgc agaaggggta gatgaagacg aagggcagca
     3901 ccgccagcgc cagccagcgg gaaaagccgt tgatgaagaa cagcagcgac gcgcacgccg
     3961 ccagctgccc gacgagaaag gccacggcct gcttcatgct gagctgcccg ctggtgagcg
     4021 ggcgaaagcg cgtgcgctcg acgtgcttgt cgatgtcgcg gtcccacatg tcgttgacgg
     4081 tgcagccgat gccgcgcatc agcagcgcgc ccagcgagaa cacgatgagc cgcagcaagg
     4141 tgggccagcc gtccgcggac tgcaccagcg cggcgatgca gggcagcagg gtcagccacg
     4201 tgccgatcgg ccggtccagc cggcacagcc gggcgtaagg ccgccacggc ttgggcagcc
     4261 agcgctcgac ccaatcgtcg aagacgatgt cgttcaggtc aggagaagaa gaggtcgtgg
     4321 aagtcatgac ggccatgcgg ctcatgaact caagacatca gaccttctgc ttgagcaact
     4381 tgcccagcac agcgatcccc gcgcggatct tctcctccgg cacggtggcg aagctcaagc
     4441 gcagggtgtt gtgctgcgcc ttgccggcga agaacggcgc gccgggcacg aaggccacgt
     4501 tctgctccac tgcttcggcc agcagctttt gcgcgtcgat gtgcgcgggc agcgtcaccc
     4561 acaggaacat gccaccctcg ggcctggtcc agctcacccc ggcgggaaac tcacgctcga
     4621 tggcctccag catgcagctg ccctgcttgc ggtagatctc gcgcacggcg ggcaggtgct
     4681 gggccaggaa gccgtccttg accgtctcgt acacggccat ctgcgtgagc gtgggcgtgt
     4741 gcagatcggt ggcctgcttg gcctgcacca gcttgtggat gatgttggcg tgcgcggcga
     4801 tgtagcccag gcgcaggccg ggggccagca ccttcgagaa cgtgcccagg cggatgatgt
     4861 tggcgccgac ctcggtgccc agcgccagca ggctgggctg tgcctcgccg gcgtagcgca
     4921 gctcgccgta ggggtcgtcc tcgatgatgg ggagcttcag gctggcggcc ttctgcacca
     4981 gggcctcgcg gcgcgcgcgg ttgagcgtgc ggccggtggg gttctggaaa ttgggcaggg
     5041 cgtacaggaa gcgcgcgccg gcggccagct cgggcgtgat cgcctcgggc accaggccgc
     5101 cttcgtcggt gggcacggtg gcgtagatgg gctggtactg gctgaacgac tgcagggcgc
     5161 ccaggtaggt ggggtcttcc accagcacct tgctgccgga gtcgatcagg atcttgccca
     5221 gcaggtcgag cgcctgctgc gaacccgaca cgatcaggat gttgtcgatc gtcaggttcg
     5281 cgccggactt gttcaggtct tgcgccaccc attcgcgcag aggcttgtag ccttcggtgg
     5341 ggccgtactg cagggcggcg cggccattgg tggccaatac cttgtcgaac gccgcgcgca
     5401 tgacctctac cggaaagccc gcgggggccg gcaggccgcc cgcgaaggaa atgacctcgg
     5461 gccgttcggt gaccttgagg atttcgcgga tggcggagct ggtgagctgg ttggcgcgtt
     5521 ccgagaagac gcactcgggt tagtaaggtt tgtccatgat ggggttcgca aaggaaagag
     5581 ctggaaacgg ccggggaaac acccgccggg ccgtctattg tcacatatag cccaggcgcc
     5641 ggcaggggcg ggccggccgg tcacccggca cgaattgcgc caatacggtt acattcgagc
     5701 gacaacaaac agccggcatg ccgaccggct ttccaggaga cgaacgcatg acggcttcgc
     5761 ccgtatccgt acccgaatat gactacatca tcgtgggcgc cggcacggcc ggctgcctgc
     5821 tggccaaccg gctgtcggcc gacccggccg cgcgcgtgct gctgctggag gctggcggcc
     5881 aggacgactg gcactggatc cacattcccg tcggctacct gtattgcatc ggcaacccgc
     5941 gcaccgactg gtgctaccgc acccaggccg atcccggcat cgccggccgc agcctgggct
     6001 atccgcgcgg ccgggtgctg ggcggcagct cgtcgatcaa cggcatgatc tacatgcgcg
     6061 gccagcgcgc cgactacgac ggctgggcgg cgcagggcaa tgccggctgg gggtgggacg
     6121 acgtgctgcc gcttttcaag gccgtcgaag accaccatgc cggcgccaat gcctttcacg
     6181 gcgccggcgg cgagtggcgc gtcgagcgcc agcgcctggc ctggtcgctg ctcgacgcct
     6241 ttcgcgccgc ggcggcgcag gccggcattg ccccggtgaa cgacttcaac cagggcgaca
     6301 acgagggttg cgattatttc gaggtcaacc agcgccgcgg cgtgcgctgg aacgcgtcca
     6361 aggcctttct tcgcccggcg ctgcgccggc ccaatctgca ggtgatcacc ggggcgcagg
     6421 tggccgggct gtcgttcgag ggccggcgcg cccggggcgt gcgctatcgc ctggccgacg
     6481 gcgtggagcg cgaggcccgc gccgtgggcg aagtggcgct ggcggccggc gccatcggtt
     6541 cggcgcaact gctgcaggtt tccggcatcg ggccgcaggc gctgctgtgc tcgctgggcg
     6601 tgccggtgct gcacgagttg cccggcgtgg gcggcaacct gcaggaccac ctgcaactgc
     6661 gcctggtcta ccaggtcagc aacgcccgca cgctcaacac catggccaat tccctgtggg
     6721 gcaaggccgc gatgggcctg caatatgccc tggcccggcg cggcccgctg gccatggcgc
     6781 cttcgcagct gggcgctttc gcccgcagcg gccccgagca ggcgcgcgcc aatgtccaat
     6841 accacgtgca acccctgtcc ctggagaagt tcggcgagcc cctgcacggc ttcccggcct
     6901 tcaccgcctc ggtctgcaat ctgcagccca aaagccgcgg ccatgtgcgc atcgccagcg
     6961 ccgatgcgcg caccgcgccc gagatcctgt gcaactacct gtccacggac gaggactgca
     7021 aggtggccgc cgacagcatc cggctcacgc gccgcatcgt gtcccagccc gcgctggcgc
     7081 cgtaccgccc cgaagagcgc aagcccggcg ccacgctgca aagcgaggcc gagctcatcg
     7141 ccgcggcccg cgagatcggc accaccatct tccaccccgt cggcacctgc aagatgggcc
     7201 atgatccgct ggccgtggtc gacgaccagt tgcgcgtgca cggcatcgaa ggcctgcgcg
     7261 tgatcgatgc gtccatcatg cccaccatca cgtcgggcaa caccaattcg cccaccctga
     7321 tgatcgcgga gaaaggcgcg cgcttgctgc gccaggcgcg cggcgggtaa aaagccccca
     7381 cgccgcgcct tcggcttgct gccccccgag ggggccgttt ttgccttggg agcggcccgg
     7441 cggcaaaaaa agcccccacg ccgcgccttc ggcttgttgc cccccgagag ggccgttttt
     7501 accttgggac ggccctgcgg taaaaaggcg tatgccgcac accatcgaaa tcgccgcgac
     7561 cctgtgcttt gccgtcgcgg tgctgcatac cttttccgtg ccgttcttcg cgcggttggc
     7621 gcatcgcggc ggcccgcatg ccggcgcctg gcacctgctg gccgaggtcg aggtcgtctt
     7681 cggcgtctgg gccttcatat tgatggtcgt catggccctg ctggccaatg tacgcgacgc
     7741 cacgcactac ctggatacgc gcaatttcac cgagccgctg ttcgtgttcg ccatcatggt
     7801 ggtggcggcc agccggccgg tgctcgagct ggtgcgcctg ctgctgcggg gcgcgctgcg
     7861 gctgctgccg gtgcgccgcg agctcggcct gttctggctg gtgatggcgc tggtgccgct
     7921 ggccggttcc ttcatcaccg agccggcggc catgacgctg gccgccatcc tgctgcgcga
     7981 aggctatctc cgcgcgcccg ggcataccgg gttcaagtat ctggcgctgg gcgtgttgtt
     8041 cgtcaatgtg tccatcggcg gggtgttgac cgcctacgcc gcgccgccgg tgctgatggt
     8101 ggccacgacc ttcggctggg acacggccta tatgttccgg cacttcggcg accgggccgt
     8161 ggtggcggtg ctggtcaacg ccttggggct ggtcctggtc tgccgcacgg ccatccttga
     8221 ttccgcgctg cagtcggccg gccagggcga aggcaaggcg ggcggccgcg cgccggtgcc
     8281 gcccctggtg atagccgtgc acctgctctt tctggtcggg gtggtgctcg cggcccacca
     8341 tcccgcggtg ttcctgggac tgctcatgct gttcatcggc ttttgccgcg cctacgagcg
     8401 ccaccaggac cgcctgttga tcaaggaagg cctgctggtg gggttcttcc tggccgggct
     8461 ggtcgtgctg ggcggcctgc agcagtggtg gctgcaggaa ctgctgggca gcctgccgcc
     8521 cctggtgctg tactggggcg ccatggcgct caccgcgctg accgacaatg cggcgctcac
     8581 ctacctgggg tcgctggtcc agggcaccag cgaagcctgg cgcgtcatgc tggtggccgg
     8641 cgccgtcacc ggcggcgggc tcacggtcat cgccaatgcc cccaacccgg ccggctacgc
     8701 cttgctcaag ggcgcctttc ccgacggcgc cgtttcggcc ggccggctct tcttggccgc
     8761 cctggcgccg accctggtcg cggcggccgc gttcctgctg atttaacgca tctgcatcag
     8821 gcgcgtttca agtacctgga gaaaatcctg ccgctgcccg gctacgatgt agacatatgt
     8881 atttccgccc acctgtttat aaatgatccg gtgatgtgaa gtctgggtca tcaggtagcc
     8941 atcgatgcct agctcaatga gctcgacaac agggacgcca ttgagcttgc cctgatccag
     9001 ggcatccatc tggtcataga cctcttccaa ggcctggtcg caaagcgccg agccccattt
     9061 ggccagcatg tagtcttgca gcgcggccag atctgcctgc gcctcggaca catggaaaac
     9121 ggtcatcagc gcatgccctt gcgtcgcttc gcaagccggg ccctggattc ctccgcggta
     9181 agcactttgc cttcgagccg gcttttctcg cccatggcga cgagcttcag gagggcgagg
     9241 cgctcctggt cctgttcgta ggtcttgatg tccatgacca ccatcttggc ttcgccgttg
     9301 tgcgtgatga tgtagggggc cgggtcttcc gcgaagcctt tggcgatgtc ggccgcatgg
     9361 gtgcgcaggt atgaaatggg tttgatctgg accgagagct tcatggcgta ctcatggatg
     9421 tatcgtatga aatttatact aaatttcata cgatagtaca agatggcgtc cgtatgccgc
     9481 cgtgcggcgc gcgacgagcc gatgccacga cggccatgcc gcccggctgt cttgccagtt
     9541 cgggctaaaa tctcccgttt ctccctcatg tctccggcgc cctggttgcg gggtgcgtgg
     9601 tccaatatgc ctatctatgc ctacaagtgc agcgcctgcg gccatgccga ggacgtgctg
     9661 cagaaaattt ccgacgcgcc cctgacggtc tgccccagct gcggccaggc cacctatgcc
     9721 aagcaggtca cggcggccgg cttccagctc aaggggtcgg gctggtatgt caccgacttc
     9781 cgcggcaatg gcggcagcgg cggcgcttcg ccgccggcca agccggctgc cgcatcgggc
     9841 gacagcggcg gctccgccgc gcccgctgcc ccggcctctc cgacgacgcc ttcgtcgtcg
     9901 tccacctgat caaggccggg cgccgtgttc aagaaattct tcatcaccgg tctgctggtc
     9961 tgggttccgc tggcgatcac cctttgggtg atcagcctgg tcgtgaccac gctcgaggcc
    10021 ttcgtgcccg gcttcctgtc ctccgaagcg ctcttcggca tgcagatccc gggcttcggc
    10081 ttcgtgctgg tgatcgtggt gatcctgctc accggcgtct tcacggccaa cttcctgggc
    10141 cgcagcctgc tggtccagtg ggagcgcctg ctcagccata ttccgctggt gcggtccatc
    10201 tacaactcgg tcaagcaagt cagcgacacc gtgctggcgc ccaacggccg cgctttccgc
    10261 caggccgtgc tcatcgaata cccgcgcaag ggttcgtgga ccatcgcctt tctcacgggc
    10321 gagcccgggg gcgaggtggc cgagcgcctg ccgggcgaac acctgagcgt gtacgtgccc
    10381 accacgccca accccacgtc gggttttttc ctcatcatgc cgcgttccga ggtcatcgaa
    10441 ttgcagatga cggtcgacgc cgcgctcaag tacatcgtct cgatgggcgt cgtggcgcca
    10501 gccgtgcatg aggcgcccga ggccctgccc gaggcgtccg atgcgcgtat cgtcgcgcaa
    10561 gcggcgccgc ccgccgaatc gtaatctttg aatccatatc ccacacggag cttttccatg
    10621 agtcgcacct gctacaccgg tcacgtttgc cgcgatcatc tcggccagac tgtcaccttg
    10681 tacggttggg tgaatcggcg ccgcgaccat ggcggcgtga tcttcatcga catgcgcgac
    10741 cgcgccggcc tggcccagat cgtgttcgat cccgacaatg ccgaagcctt caagatcgcc
    10801 gaaagcctgc gcaacgaatt ctgcatccgc atcaccggcc tggtgcgcga gcgccccgag
    10861 ggcacgacca accccgagct ggcctcgggc gaagtcgaga ttctctgccg cgaggtcgaa
    10921 atcctcaacg cctcggtcac gccgcccttc cagctcgacg acgacaacct gtcggaaacc
    10981 acgcgcctga cgcaccgcgt gctcgacctg cgccgctcgc agatgcagcg caacctgatg
    11041 ctgcgctacc gcgtctccat cgaagtgcgc aaatacctcg acgagctggg cttcatcgac
    11101 atcgaaaccc ccatgctcac caaga
//
LOCUS       DASWPO010000296        22760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_247943, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000296 DASWPO010000000
VERSION     DASWPO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 22760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22760
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247943"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..923)
                     /gene="bcsP"
                     /locus_tag="VGC69_15515"
     CDS             complement(69..923)
                     /gene="bcsP"
                     /locus_tag="VGC69_15515"
                     /inference="COORDINATES: protein motif:HMM:NF040718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsP"
                     /protein_id="HEY0296737.1"
                     /translation="MSRSTDITNLYQWTGGNPSQYQEVGHAEQAQASGERWPMLSAVR
                     PNRAAVVPVMGGPVVASDAPASMPVETVAQVAQAAQPIAPQAASPWGEPVVVPPLAAQ
                     PQAVQPMALQDVPRRVEPGLVVHEFERALNEMPTAAPAAPVVPAAPVSQPVSPTVFAP
                     PPASAMPFAPPMSPVEMSAPPMTQAVPEPLAVPAAPFEPPVAPVAVSPAVMPSVLEPA
                     IVPVMPEIAQAVQAVPEPAAAPSMPEPVVPAPAAVPAQPSSLSGVFARLARAAPVADA
                     AQGKGSSW"
     gene            complement(1030..1512)
                     /locus_tag="VGC69_15520"
     CDS             complement(1030..1512)
                     /locus_tag="VGC69_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HEY0296738.1"
                     /translation="MVLPEELVDNAIAVSHYINNRLCSPQWRAFLSIFATELTQDLTS
                     NHLGKVMRRTGEHFARDHALANAGTITDLQAAINRVWDALDWGWVEIGEAEDKLVLTH
                     YYAPLQAAFGADNQAWSGAFLEGAYEWWMRQLGADPELRVTSARDSGALGATVFHFGK
                     "
     gene            2257..4470
                     /locus_tag="VGC69_15525"
     CDS             2257..4470
                     /locus_tag="VGC69_15525"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY0296739.1"
                     /translation="MTAPNPLLAPTADLIDYAAVRPEHIEPAVDELLALARKAVDEAA
                     SPGLPATWEAVVEPLDGTSERLWRAWSVAGHLNAVVNTPELREAYNAALPKVTEFSTW
                     VGLHEGLYRQYQRLHAEPGFAAWPAVRRRVVDLALRDFRLSGVQLQGADRERYAEISD
                     REAQASQKFSENALDAIDAWSLFLEDASRLDGLPEDVLAAAREAAQAEQEKGPHVAPD
                     GLLPPEGASPALGRPGGGRSKPGYKLTLKMPCYLPVMQYARDRALRETLYRAYGTVAS
                     DQGDPKYDNSPLIEELLALRAEEARLLGYHDFAELRLQTRMARSADEVVKFLRDLARR
                     AKPYAERDLDQLRDYAARALGLPDLQAWDIAYASERLRESRYAYSEDELKQYFTEPRV
                     LAGLFEVIESLFDVRLRETPVSAWHPDVRGVRVENPSGELVGYLYLDLYARAGKQGGA
                     WVDSERSRRLAAGRLQTPVVYLTCNFARPGGSVQDPGVQAPQAAPDGLPPGGKRPALL
                     THDDVITLFHETGHALHALLSEVDEPGAAAFASVEWDAIELPSQFMENFCWEWPVLQK
                     LSAHVENGEPLPRALYDRLLAARNFQSGMQTVRQIEFALFDMQMHASAQGLPVAQVLE
                     LLQQVRQEVAVLFPPAWHRLPHNFSHLFSGGYGAGYYSYKWAEVLSADAYAAFEEAAQ
                     ASRQGPLDAATGKRFRQEILAVGGARPAAESFQAFRGRAPSIDALLRHSGMAAEA"
     gene            4575..4826
                     /locus_tag="VGC69_15530"
     CDS             4575..4826
                     /locus_tag="VGC69_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0296740.1"
                     /translation="MHVIIMIIVGFIVGLLARAVMPGNQNMGIIMTTILGIIGAVVAG
                     YLGQAMGWYAPGQPAGWIGSVVGAIIVLFVVGLIQKMRA"
     gene            complement(4934..6400)
                     /locus_tag="VGC69_15535"
     CDS             complement(4934..6400)
                     /locus_tag="VGC69_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0296741.1"
                     /translation="MRTAKLLPLSLALAGVLAGCTLAPTYERPAAPVEGNYPTGPAYV
                     HAQPGASVQGAAADVGWREFFQDPLLQRLIEMSLANNRDLRVAMLNVEVTRDQYRIER
                     ASLVPAASVSASGTAQRVPADLATSGSITDSHNWQVGPSVSWELDIFGRIRSLSDAAL
                     QTYFAQDETRVATQLSLIAATASSYLTLRADQELFSLTIETLKTRQESYNLVKQSYDQ
                     GVSSALDLSQAETALRTAESSRAQYMRLVAQDRNALVLLLGEPLPADVSAQLDADPRL
                     HDDILPLELPAGLPSDLLERRPDIRAAEHQLLSANANIGAARAAFFPTFTLTGFAGTA
                     SASWSGVFDPGSEAWSFAPKITIPIFQGGALLANLDVSKVRKNIQIATYEKAIQTGFR
                     EVADTLAGRGTLDDQVRAERQLVDASELAYKLSQQRFDSGVDSYLSVLDSERSLYTAQ
                     QTLIGTRLERLNNQINLYKALGGGWAEHTVAAGTPPQS"
     gene            complement(6412..9594)
                     /locus_tag="VGC69_15540"
     CDS             complement(6412..9594)
                     /locus_tag="VGC69_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0296742.1"
                     /translation="MAKFFIDRPVFAWVIAIVLMLAGLLAIRTMPVAQYPNIAPPAIA
                     VQVTYPGASAQTVQDTVVQIIEQQLNGIDHLEYLFSESNNDGSMTITLTFAQGTNPDI
                     AQVQVQNKLAVAQPLLPLEVQQQGIRITKATKNFLMVAAFISRDRSLNRADLGDYVAS
                     YIQDPISRTEGVGDFQLFGSSYAMRVWLNPAKLVSYGLTASDVTKAIQDQNVQIASGQ
                     LGQLPAVPGQQLNASVIGPSRLNTPEQFRNILLKVNPDGSQVRVSDVAQVELGPQAYT
                     IDSTYNGRPASGLAIKLSPGANALDTAVNVRNTINKLKPFFPPGVDVVYPYDTTPFVK
                     LSIDDVVETLIEAIVLVFLVMYLFLQNIRATLVPTLAVPVVLLGAFGVLAAFGYTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMHEDHLSPLQATRKSMEQITGALVGIAMVLA
                     AVFIPMAFFGGSTGVIYRQFSITIVSSMALSVLVAIVFTPALCATILRPPSHAPKKGF
                     FGWFNRSFDRASEKYGNVVSRVLSKSKRIYVIYVLIVIGMAYLFTHIPTAFLPEEDQG
                     ILFAQVSAPPGATTERTAQSINEASNYLLNDEKDAVQSIFMISGFNFGGRGQNAAIMF
                     IKLKDWDERKTANLRAAAVAARANVKFARDIHDAQIAVFAPPAVMELGNATGFDFMLQ
                     DQAGVGHEALLAARNQVLAEAMKDPRLVAVRPNGIEDAPQYILDIDREKAKALGVAVA
                     DLDDVLSVAWGSSYVNDFIDRGRVKKVYVMGAADSRMQPEDLNKWYVRNAAGGMVPFS
                     AFASAHWSFGAQKLNRYNGVPSFEIQGQPAPGYSSGDAMTAMEGIASKLPTGIGFDWT
                     GLSFEERSSGQQAPALYAISLIVVFLCLAALYESWSIPTAVMLVVPLGVVGALLATML
                     RGLSNDVYFQVGLLTTVGLAAKNAILVVEFAKENFEHGGSLVEAAIHAARQRLRPILM
                     TSMAFILGVLPLALSKGAGSGSQNAIGTGVIGGMLTGTFLAIFFVPTFFVTVLHLFKV
                     KRPSEKLDKYAKPDAAPGAPSVETSAGDHS"
     gene            complement(9607..10779)
                     /locus_tag="VGC69_15545"
     CDS             complement(9607..10779)
                     /locus_tag="VGC69_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0296743.1"
                     /translation="MSVLALALAVGLAACGKQQDSAAQAAAKPEVWVVTLKTQPVTLT
                     TELPGRTAAFREAEVRPQVNGILQKRLFTEGGSVKAQQQLYQIDPAIYQAQFDSSKAS
                     LARAEASQRSAASLADRYKPLVETRAVSRQAYDDAVAASQQAAADVLSAKAALETARV
                     NLVYTKVLSPIDGIIGRSAVTEGALVTANQTSPIATIQQIDPMYVDVAQSSVQLLRLK
                     SALASGQLKSSDGQAADVKLTLEDGTQYPLPGKLQFSEVTVDPGTGSVTLRALFPNPD
                     HSLLPGMFVRAILGDGVAPQGLLVPQRGVTRSPRGIPTALVVTPENKVELRQIKTDRA
                     VGDQWLVTDGLKAGDKVIVEGLQRVRPGVDVTATEWTPPVPVAVKSPDQSAQQAKQ"
     gene            complement(11163..11894)
                     /locus_tag="VGC69_15550"
     CDS             complement(11163..11894)
                     /locus_tag="VGC69_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEY0296744.1"
                     /translation="MEKVRISKLMSERGLCSRREADAYIERGWVKVDGIVVSELGARA
                     YPDQGITLERAAQARQTARVTILLNKPVGYVSGQAEKGYTPAVALISERSRHAGDRTA
                     LRFARAHLQGLAVAGRLDIDSQGLLVLTQDGRVARHLIGEDSDVDKEYLVRVQGRLSE
                     SGLALLNHGLSLDGKPLRPAQVRWQNADQLCFVLREGKKRQIRRMCELVGLKVVGLKR
                     VRIGRVALGDLPAGQWRYLRDDENF"
     gene            11967..12530
                     /locus_tag="VGC69_15555"
     CDS             11967..12530
                     /locus_tag="VGC69_15555"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEY0296745.1"
                     /translation="MTDLPCPASPLGWHAAPPPSLPARHKAWLFRPGALTAGLRSLGA
                     MDLRVLAEYAAGVPADEAHGMRLAPGTPVWVREIAMAVDGRDAVVARSLAPLAASHGV
                     WQGMRRLRTRPLADMLYHDRSIRRSPFACARLGYPLAQHRTVLGVDAGYAREALWARR
                     SVFWRFGQPLLVAECFVPGFWQDILNA"
     gene            12670..13908
                     /locus_tag="VGC69_15560"
     CDS             12670..13908
                     /locus_tag="VGC69_15560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0296746.1"
                     /translation="MKSIAVIGAGIVGLCTAANLQRKGLRTTLIDSLPPGTSTTYGNA
                     GMISANSITPVAMPGMLRKVPKWLRDPEGPLFVDPKYLPSALPWLMRWIRAGASMEQV
                     RRSSQGLYQLHSHALDEYKKLLGTEHFDALVKTVGQIHLWDSPQLPDNEKIAASMRSE
                     AGIETRALSSGDLRDMLPELTSSVGCALYFPNNGHTTSPLRLAQTIHGLFTEAGGQTA
                     PEKVLRIVPQGSQYRLWTNCGDHSYDQVVVAGGAWSLALLKPLGLDFPLETERGYHVQ
                     VDAPNIDLPYPILHKSRGFGASVMEDGLRLAGTVEIAGLRKPPNARRTESLLRHAKVL
                     FPRLEIGKHKIWMGYRPSMPDSLPVLAASSRYPGLFIACGHGHTGITAGATTGRLMAE
                     IVTQSPTFIDPAPYRLERFS"
     gene            13910..15169
                     /locus_tag="VGC69_15565"
     CDS             13910..15169
                     /locus_tag="VGC69_15565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0296747.1"
                     /translation="MNRGKEARPATIAVIGAGIIGLCTAANLQRKGLQVTVLDPMPPG
                     TNATYGNGGMISTAAITPVALPGMLRKVPKWLRDPEGPLFVAPRYLPVALPWLMRWVR
                     AGALDQVRHAAEALHHLHDHAITEYRQLLGPEHFNSVVKVLGQVHMWDNKEWPESEKI
                     AAAFRDQYGIATEELSPERLRDMLPELATSVSRALYFPNNGHTTNPLRLAQVIHGLFM
                     DDAGQSLREKVLRITPEDGRYRLWTNCGDHIFDQVVVAGGAWSLDLLAPLGLGFPLET
                     ERGYHVQIANPKIEVAYPILHKGRGFGGSMMEDGLRLGGTVEIAGLDMPPNPRRIQAI
                     QKHAKVVQPRLDLSEYKVWMGFRPSTPDSLPVMGESRRHPGLHIACGHGHLGITGGAA
                     SGRLMAEIITKSPTFIDPTPYRLERFL"
     gene            15182..16270
                     /locus_tag="VGC69_15570"
     CDS             15182..16270
                     /locus_tag="VGC69_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296748.1"
                     /translation="MKQQITKPHSATRRSILGMAGAALTTPYLWIPRANAAGQVIIRS
                     PGGVYDDIRKETIYDPFRKETGIEVIPVATTGAKLDAMLRSGNIELDIVDTGIQLLVS
                     YQKQDALIPIPYKEFKYTDPDDIDENYKHEYIVGNFTYAQAMAFNTEAYTLATGPKDW
                     AEFWDAKRFPGPRMLADVATGSPDLEAALIADGVPMDKLYPLDLKRAFDSLTRIRPSI
                     RKFWDTGSLCAQMLTNKEVVMGSIWSSRLLASREAGAPLVQNWNQHEVQVQAYSILKG
                     APNTENAKKLIDYCLSKDVQQRYFERYSAGPVTKTAYKNLSADKRENIPGGDRTARYG
                     FVQDAQWWGNLENKQAVSAAWSKWVLSK"
     gene            16201..17292
                     /locus_tag="VGC69_15575"
     CDS             16201..17292
                     /locus_tag="VGC69_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296749.1"
                     /translation="MVGKPGKQTGSQRCLVQMGALQVKGAEITLEGLTKQYYGHAVVE
                     DVSAVIPAGSFFSLLGPSGSGKTSTLMMLAGFVSPDTGRILLNGRDITADTPQQRGLG
                     MVFQNYALFPHLTVEENVAFPLRVRRKKPQEIREKVRWALDVVRLSPYADRHPKQLSG
                     GQQQRVALARAIVFEPGVVLMDEPLGALDKNLRYHMQVEIKEIQQRLGMTVVYVTHDQ
                     EEAMNMSDGIAIMDQGRIVQMDSARRIYEDPRSQFVAKFLGEANLIPAERLESHAGRK
                     GESLFVRPERIRLDSTNAATPVDSTRVNGMVSRASFLGNIVRYSVDLGAGCQITADIH
                     NNGGERQFSVGEGVAVLWRHADSKLLADD"
     gene            17285..18163
                     /locus_tag="VGC69_15580"
     CDS             17285..18163
                     /locus_tag="VGC69_15580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296750.1"
                     /translation="MTDTTLAPRARSPLPSSLSGTGMLFVPLALMLLLIHVGPLIWFF
                     IKTFASVQQGFLQQISDVVLSVDMLQVIWTTLWTSSLVTLLTLLLAYPVAYSLCKAGS
                     KFTMAGILACIIVPYFTSVIVRTYSWMVLLGHNGLINRFLLDLGLIDEPLHLMYNQAG
                     VLIGMVYVLLPYLVLTLYSTMKSIDDNLLRAASGMGASGAYAFFTVFWPLSLPGVVSG
                     GLIVYILALGFFITPSLMGSSNDVMIAMLIQREVEVDMNWGLAAVLSLVLLVMTLILY
                     IIYCRYTNIDQMMGRK"
     gene            18175..18957
                     /locus_tag="VGC69_15585"
     CDS             18175..18957
                     /locus_tag="VGC69_15585"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296751.1"
                     /translation="MKAMTWINTLTTLLCVILIAPIVLIVILSFSGDAIMQFPPHSFS
                     LRWYTDFFADTRWRKALYSSLTIGLGACLIATFVGFLAAYALVRGQFKAKKLMLSFVL
                     LPLIIPNIITAIALYFLSAKLGLIGAKPWISVTHAVIGLPIVVMILISALQSVDENIE
                     RAAYSLGASRSMTFVKVVIPLALPGVVSAALFSFLTSFDELVIAMFLSGLSSETLQVR
                     IWNSLQMDAEPIIASVSALLIAVTVILLGVDALLRHKRARRA"
     gene            18980..19363
                     /locus_tag="VGC69_15590"
     CDS             18980..19363
                     /locus_tag="VGC69_15590"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0296752.1"
                     /translation="MIEVVETGIAKSKGPVSGAVIGTGARTLYMAVIPKDPQSGAIVN
                     GDIETQARQTFANMKQTVEAAGGTLKDMAQVLIYLIEGSDAAAMNKVYGEFFSAPYPN
                     RATVVVKELLAPGMRIEVVAHAALP"
     gene            19469..20428
                     /locus_tag="VGC69_15595"
     CDS             19469..20428
                     /locus_tag="VGC69_15595"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296753.1"
                     /translation="MNFRHVEVIRAVVLTGTVTGAANRLHVTQPAVSNILRDAEERLG
                     FVLFERAGGRITPTKKAALLVAEIERAFTGLDAINEVCGHLVRNENRRISIASTPSWA
                     ASVLPSVMRRYRDRFPDVRFSLLARSSDMVQSMVMSQKADIGFGLEVPLIPGVRQELV
                     VRSLIRCIVSENHPLANKRQVGPKDLHGHDMISFSHLNEGTEDTYSDMFEQASSYPES
                     VVDCATSITTCALVESGLGFALVHQAVLHLFKHARVRVIPFVPTTTLDMCAYVSEAHD
                     TSALNLDYMIELAREQAHTLETDISRLAEARDTSKSPLRRKVA"
     gene            complement(20484..21449)
                     /locus_tag="VGC69_15600"
     CDS             complement(20484..21449)
                     /locus_tag="VGC69_15600"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296754.1"
                     /translation="MKYKHGLTMAAALSCAAMNTAQAAWPDDRPIEIIVGYSAGSATD
                     LMARNLAPFLARYLGGHAQVLVIDKPGATGAISNAYVQHSKPDGYTLGTVNLPGFLFT
                     PMYQSTPYQPDGMTLVARLVADPAVLIARDDNPAADLAGVVQAMRQKPGQVSFGNNGV
                     GTNGHLTLLQLQQATHTQGIAVPFKGSGETSTALMGGQIDYAILSASEATMMRQNPVK
                     VVAQFAPAGERGQALPTVPTAQEQGYAVLMTSERGLAGPKGLPSGIIDRLNAAISASL
                     KDPDYRKAAANDLPVLAYLPGGQWDASMKDTEQRLRKLVPLMPKQ"
     gene            complement(21468..22439)
                     /locus_tag="VGC69_15605"
     CDS             complement(21468..22439)
                     /locus_tag="VGC69_15605"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296755.1"
                     /translation="MKTLHRIAALFGVAAGLLTASACAADAYPDHPITLIVPWNAGGA
                     IDVTGRKLAEMLGKAGVTIVVENIPGASSIIGLGRVARSAPDGYTFGLATPSLMGAIA
                     LKTTKITQDDFSVLNKVGVDPEILVVPKSSPANSVEDFVKLMKDKPAGVSVGIPGSNN
                     VNQIFAAMLAQAVNVQYTNVPYTGGSKVLIDVAGGQLDAGVVKPSEAIGQIKSGLIKV
                     IGVFARQRVDALPDVPTFAEHHVDVFPYGDLEQVTYLVAPKGVPQDRLAKMEALFAKA
                     ITSDDYKQFTNDYGMSITDVRGAAMGKEVQGIQKTFDEVAPKIFTAQ"
     gene            complement(22484..>22760)
                     /locus_tag="VGC69_15610"
     CDS             complement(22484..>22760)
                     /locus_tag="VGC69_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296756.1"
                     /translation="VPDADRVMRDWFARHQAQPINHLLAVKSDLLREHPWLAGELAAL
                     FERARRHAGGDDPAPWSRAGASLLLAFCARQGLTPSAYSPQALVAGG"
BASE COUNT         4097 a         7383 c         7181 g         4099 t
ORIGIN      
        1 cacgcccagg ttggcggcca cggtgctctt gcccacgccg cccttggcgg ccacgacggc
       61 gatgaccgtt accatgacga tcccttgcct tgcgctgcgt ccgcaaccgg cgcggcccgc
      121 gccagccggg cgaacacacc gctcaggctc gacggctgcg cgggaacggc ggcaggcgcc
      181 gggacgaccg gttcgggcat gctgggagca gccgccggct cgggcacggc ctggacggcc
      241 tgcgctattt caggcatgac aggaacgatg gcgggctcga gcacggacgg catgactgcc
      301 ggcgataccg ctacgggcgc taccggcggt tcgaacggcg cggcagggac cgccagcggc
      361 tcgggcacag cctgggtcat cggcggcgcg gacatctcca cgggagacat cggcggcgcg
      421 aacggcatgg cagaggccgg cggcggcgca aacacagtag gagagactgg ctgcgaaaca
      481 ggcgcggcgg gaacgacagg tgcggcaggc gccgctgtcg gcatctcgtt caaggcccgc
      541 tcgaactcgt ggaccaccaa cccgggctcg acacgccgcg gcacgtcttg caaggccatg
      601 ggctgcacgg cttgcggttg ggcggccagg ggcgggacaa ccacgggctc gccccagggc
      661 gaggcggcct gcggcgcgat aggctgcgct gcctgcgcga cctgtgccac cgtctcgacg
      721 ggcatgcttg ccggcgcgtc actcgcaacc accggccccc ccatcaccgg caccaccgcg
      781 gcgcggttcg gccgcacggc ggacagcatc ggccagcgct cgcccgaggc ctgggcctgc
      841 tccgcgtgcc ccacttcctg gtactgcgac ggatttcccc ccgtccattg gtaaagattc
      901 gtgatgtccg tggatctgct catgcgtcac cttgcgccgt atgctgctca catctatcgg
      961 aaacctgcgc gccatggcca gcacgcgccg gaatgacgcc tgcggctcgc gcgctgcagg
     1021 cttgccgcct catttgccga aatggaaaac cgtcgcgccc agcgcgccgg aatcgcgtgc
     1081 cgaggtcacc cgcaattccg gatcggcccc caactggcgc atccaccatt cgtaggcgcc
     1141 ttccaggaag gcgccggacc aagcctggtt atctgcaccg aacgccgcct gcaagggcgc
     1201 gtagtaatgg gtcaacacga gcttgtcttc ggcttcgccg atctcaaccc agccccagtc
     1261 cagcgcgtcc cagacccggt tgatggccgc ctgcagatcg gtgatcgtgc cggcgttggc
     1321 cagcgcgtga tcgcgggcga agtgctcgcc tgtgcggcgc atcaccttgc ccagatggtt
     1381 ggatgtcaga tcctgcgtca attccgtcgc gaaaatcgac aggaatgcgc gccactgggg
     1441 cgaacacagg cggttgttga tgtagtggct gacagcgatg gcgttatcca caagttcctc
     1501 cggcaagacc atcgacacgg catgtccgtg tcaggcggac atacctaagg ctcccgcagc
     1561 cgtgggctgc gaggatctgg aatcaattag tgattgcgtg ccgcaccagg cttgcgcccg
     1621 ggacccggcc gacgacaaac ccggccggca tgatcatgcg ccgggagaca ttcgggtcgg
     1681 acgcgcgatg gcaagaaaac ggaatgaacg aacgcttggc ttgaaagcgt cgggctgagc
     1741 ttctgaaaac agtcacggca gttctgccat atctggcaag gcggagctgc ggttttctgg
     1801 ccgcattctt gaagcataac cttgcgtctt cagcgcatgt ccccggatct ccgtgatgtt
     1861 ggggccacat gccattggct attctaatgg ctacatcaat tgataccaat aaattatttt
     1921 tacgggtttc agactacatt taaataatta cttaaccaaa tattgacaat tgcaggtaac
     1981 aaaagcacgc ctccgctatg aagaagcgcc ggttttagcc cccatatcgt gccataaagg
     2041 ccctgcttgc cccgatcgtt accaagggtt ttccttagtt ccaatcatca ccaattctgt
     2101 cgttttttac gatttcgcca ttccgtctcg ccgccgcatc cggcgacgcg cagccattcc
     2161 ggcggccgcg cgccgtttcg agcgttcgcc gcgcttgcct tcggcctggg cgtccccata
     2221 tggttaactg tcccttcttc acacgccgca cggaacatga ccgcccccaa tccgctactc
     2281 gcccccaccg ccgacctgat cgactacgcc gcggtccggc ccgaacacat cgagcccgct
     2341 gtcgacgaac tgctggcgct ggccaggaag gccgtggacg aggcggcctc gcccggcctg
     2401 cccgccacct gggaggccgt ggtcgagccg ctggacggca cctccgagcg cctgtggcgc
     2461 gcctggtcgg tggccggcca cctcaacgcc gtggtcaata cgcccgagct gcgcgaagcc
     2521 tacaacgccg cgctgcccaa agtcaccgaa ttctccacct gggtgggcct gcatgaaggc
     2581 ctgtaccgcc aataccagcg cctgcacgcc gagcccggct tcgccgcctg gccggcggta
     2641 cgccggcgcg tggtcgacct ggccctgcgc gatttccgct tgagcggcgt gcaactgcag
     2701 ggcgccgacc gcgagcgcta cgccgagatc tccgaccgcg aagcgcaggc ttcgcagaag
     2761 ttttccgaga acgccctgga cgccatcgat gcctggtcgc tcttcctcga agacgcgagc
     2821 cgcctcgacg gcctgcccga agacgtgctg gcggccgcgc gcgaagcggc tcaagcggaa
     2881 caagaaaaag gcccccacgt tgcgcctgac ggcttgctgc cccccgaggg ggcctcgccc
     2941 gccttggggc ggcccggcgg cgggcggagc aaacccggct acaagctcac gctgaaaatg
     3001 ccctgctatc tgcccgtgat gcagtacgcg cgcgaccgcg cgctgcgcga gacgctgtac
     3061 cgcgcctatg gcaccgtggc gtccgaccag ggcgatccga agtacgacaa ctccccgctc
     3121 atcgaggaac tgctggccct gcgcgccgaa gaggccaggc tgctgggcta ccacgacttc
     3181 gccgagctgc gcctgcagac ccgcatggcc cgctccgccg acgaggtggt caagttcctg
     3241 cgcgacctcg cccgccgcgc caaaccctat gccgagcgcg acctggacca gttgcgcgac
     3301 tacgcggccc gcgcgctggg actgcccgac ctgcaggcct gggacatcgc ctacgcctcg
     3361 gaacgcctgc gcgaatcccg ctacgcctat tccgaggacg agctcaagca atacttcacc
     3421 gagccgcgcg tgctggccgg gctgttcgag gtgatcgaat cgctgttcga cgtgcgcctg
     3481 cgcgagacgc ccgtgtcggc ctggcatccc gacgtgcgcg gcgtgcgggt cgagaacccg
     3541 tccggcgagt tggtgggcta tctgtatctg gacctgtacg cgcgcgccgg caagcagggc
     3601 ggcgcctggg tcgacagcga gcgcagccgc cgcctggcgg ccggccggct gcagacgccg
     3661 gtggtctacc tcacctgcaa tttcgcgcgg cccggcggca gtgttcaaga ccccggcgtt
     3721 caagcccccc aagccgcgcc tgacggtttg ccgcccggcg gcaagcggcc tgccctgctc
     3781 acccacgacg acgtgatcac cctcttccac gaaaccggcc atgccctgca tgccctgctc
     3841 tccgaggtgg acgagccggg cgccgcagcc ttcgccagcg tggaatggga cgccatcgag
     3901 ctgccctcgc agttcatgga gaacttctgc tgggaatggc cggtgctgca aaagctgtcg
     3961 gcccatgtgg aaaacggcga accgctgccg cgcgcgctct acgaccgcct gctggccgcc
     4021 cgcaatttcc agagcggcat gcagaccgtg cggcaaatcg aattcgcgct cttcgacatg
     4081 cagatgcatg ccagcgccca gggcttgccc gtcgcccagg tgcttgaact gctgcagcaa
     4141 gtgcgccagg aagtcgccgt gctcttcccg cccgcctggc accgcctgcc gcacaatttt
     4201 tcgcacctgt tctccggcgg ctacggggcc ggctattaca gctacaagtg ggctgaagtg
     4261 ctctcggccg acgcctatgc cgctttcgag gaggccgccc aggcgtcccg ccaggggccg
     4321 ctggatgccg cgaccggcaa gcgctttcgc caggaaatcc tcgccgtcgg gggcgcccgg
     4381 ccggcggcgg aatccttcca ggccttccgc ggccgggcgc ccagcatcga cgccctgctg
     4441 cgccatagcg gcatggccgc cgaggcctga tgccccttct acgggttgtt acacatatgg
     4501 caaggagtca gggacaggct tgggtatgat cgtcgctgca cggattcccc ctccctttat
     4561 gtaggagtac acgcatgcac gtcatcatca tgatcatcgt cggtttcatc gttggcctgc
     4621 tcgcccgcgc ggtcatgccc ggcaaccaga acatgggcat catcatgacc acgatcctgg
     4681 gcattatcgg cgccgtggtg gccggttacc tgggccaggc catgggctgg tacgctcccg
     4741 gccagcctgc cggctggatc ggctcggtgg ttggcgcgat catcgtcctg ttcgtggtcg
     4801 gcctgatcca gaaaatgcgg gcttagtcct gagccgcgac gggtttcacg gccccatgaa
     4861 aaacggcccc ctacaaaagg gggccgtttt gctttgatgg gcccgttgct tcaggccacc
     4921 cggcagcgat ccatcaggat tgcggcggcg tgcccgccgc caccgtgtgc tcggcccagc
     4981 cgccgcccag ggccttgtac aggttgatct ggttgttcag gcgttccagg cgcgtgccga
     5041 tcagggtctg ctgggccgta taaagcgaac gctccgaatc cagcacgctg agatagctgt
     5101 ccacgcccga gtcgaagcgc tgctgcgaca gcttgtaggc cagttcgctg gcatcgacga
     5161 gctggcgctc cgcgcggacc tggtcgtcca gcgtgccgcg gccggccagc gtatcggcca
     5221 cttcgcggaa accggtctgg atagcctttt cgtaggtggc gatctggatg ttcttgcgca
     5281 ctttggacac atccaggttg gccagcagcg ccccgccctg gaagatcggt atcgtgatct
     5341 tgggcgcgaa gctccaggct tccgaccccg gatcgaacac gcccgaccag gaggcgctgg
     5401 ccgtgccggc gaaacccgtc aaggtgaacg tggggaagaa agccgcccgc gcggcgccga
     5461 tattggcgtt ggcggaaagc agctggtgtt cggcggcccg gatgtcgggc cggcgctcga
     5521 gcaggtcgga cggcaggccg gcaggcagtt ccagcggcag gatgtcgtcg tgcaggcgcg
     5581 ggtcggcatc gagctgcgcc gacacgtcgg ccggcagggg ttcgcccaat agcagcacca
     5641 gggcattgcg atcctgcgcg accaggcgca tgtactgcgc ccggctgctc tcggccgtgc
     5701 gcagggccgt ctcggcctgg ctcaggtcga gggccgacga cacgccctgg tcgtagctct
     5761 gcttgaccag gttgtacgac tcctggcggg ttttcagcgt ctcgatcgtg agggagaaca
     5821 gctcctggtc ggcgcgcagc gtgaggtagg acgaagccgt cgcggcgatc aggctgagct
     5881 gcgtggcgac gcgggtttcg tcctgggcga agtaagtctg caaggcggcg tcgctcaggc
     5941 tgcggatgcg gccgaagatg tccagttccc acgataccga gggcccgacc tgccagttgt
     6001 gggagtcggt gatgctgccc gaggtagcca ggtcggccgg aacgcgctgc gccgttccgg
     6061 acgcgctgac gctggccgcc ggcaccagac tggcgcgctc gatacggtac tggtcgcgcg
     6121 tgacctcgac gttgagcatg gccactcgca aatcgcggtt gttggccagg gacatctcga
     6181 tcaggcgctg cagcagcggg tcctggaaaa actcgcgcca gcccacatcg gcggcggcgc
     6241 cctgcaccga ggcgcccggc tgtgcatgca cataggccgg gccggtgggg tagttgcctt
     6301 ccaccggcgc ggcgggccgt tcataggtgg gcgccagggt acacccggcc agcacgccgg
     6361 ccagggcaag ggacaaaggc agaagcttcg cggttctcat cgaaaacatc atcacgaatg
     6421 gtctccggcg gacgtttcga ccgaaggtgc gccgggagcg gcgtcgggct tggcatattt
     6481 atcgagcttc tcgctcgggc gcttgacctt gaacaggtgc agcaccgtga cgaagaaggt
     6541 gggcacgaag aagatcgcca ggaacgtacc cgtcagcatg ccgccgatca cgccggtgcc
     6601 gatggcgttc tggctgccgg agcccgcgcc cttggacagc gccagcggca gcacgcccag
     6661 gatgaaggcc atggacgtca tcaggatggg acgcaaacgc tggcgcgccg cgtggatggc
     6721 cgcctcgacc aggctgccgc catgctcgaa gttctccttg gcgaattcca cgaccagaat
     6781 ggcgttcttg gccgccaggc ccaccgtggt gagcaggccc acctggaagt acacgtcgtt
     6841 ggacaggccc cgcagcatcg tggccagcag cgcgccgacc acgcccagcg gcaccaccag
     6901 catcaccgcc gtggggatgc tccagctttc gtacagcgcg gccaggcaca ggaacaccac
     6961 gatcagcgag atggcgtaga gcgccggcgc ctgctggccg gacgagcgtt cttcgaacga
     7021 caggcccgtc cagtcgaagc cgatgcccgt gggcagcttg gacgcgatgc cctccatggc
     7081 agtcatggca tcgcccgagc tgtagcccgg cgccggctgg ccctggattt cgaaggacgg
     7141 cacgccgttg tagcggttga gcttttgcgc gccgaagctc cagtgggcgc tggcaaaggc
     7201 cgagaacggc accatgccgc cggcggcgtt gcgcacgtac cacttgttca ggtcctccgg
     7261 ctgcatgcgc gaatccgcgg cacccatcac gtagaccttc ttgacgcggc cgcggtcgat
     7321 gaagtcgttg acgtacgacg acccccaggc gaccgacagc acgtcgtcga gatcggcgac
     7381 cgccacgccc agcgccttgg ccttctcccg gtcgatgtcg aggatgtact gcggcgcatc
     7441 ttcgatgccg ttgggacgca cggcgaccag cctgggatcc ttcatggcct cggccagcac
     7501 ttggttgcgc gcggccagca gggcctcgtg gcccacgccc gcctggtcct ggagcatgaa
     7561 gtcgaagccc gtggcgttgc ccagttccat caccgcgggc ggcgcgaaca ccgcgatctg
     7621 cgcgtcgtgg atgtcgcggg cgaacttgac gttggcccgc gccgccacgg ccgcggcgcg
     7681 cagattggcc gtcttgcgct cgtcccagtc cttcagcttg atgaacatga tggccgcgtt
     7741 ctggccccgg ccgccgaagt tgaacccgga gatcatgaag atcgactgca ccgcgtcctt
     7801 ctcgtcgttg agcaggtagt tgctggcctc gttgatggat tgcgcggtgc gctcggtcgt
     7861 cgcgccaggc ggcgcggaca cctgggcgaa caggatgccc tggtcctctt ccggcaggaa
     7921 ggccgtgggg atgtgcgtga acagataggc catgccgatc acgatcagca cataaatgac
     7981 gtagatgcgc ttgctcttgc tcagcacgcg cgacacgaca ttgccgtatt tttcgctggc
     8041 ccggtcgaag ctgcggttga accagccgaa gaagcccttc ttgggcgcat gcgacggcgg
     8101 acgcaggatg gtggcgcaca gcgccggggt gaacacgatg gccacgagca ccgacagcgc
     8161 catggacgac acgatggtga tggagaactg ccggtagatc acgcccgtgg aaccgccgaa
     8221 gaaggccatg gggatgaaca cggccgccag caccatggcg atgccgacca gcgcgccggt
     8281 gatctgctcc atggacttgc gcgtggcctg cagcggcgac aggtggtctt cgtgcatcac
     8341 gcgctcgacg ttctccacca ccacgatggc gtcgtccacc agcaggccga tggccagcac
     8401 catgccgaac atggtgaggg tgttgatggt gtagccgaaa gccgccagca cgccgaaggc
     8461 gcccagcagc accaccggca ccgccagggt cggcaccagc gtggcgcgga tgttctgcag
     8521 gaacagatac atcaccagga acaccagcac gatggcctcg atcagcgtct cgaccacgtc
     8581 gtcgatggac agcttcacga agggcgtggt gtcgtaggga tagaccacgt ccacgcccgg
     8641 cgggaagaag ggcttgagct tgttgatggt gttgcgcaca ttcacggccg tgtccagcgc
     8701 gttggcgccc ggcgacagct tgatggccag gcccgaagcg ggacggccgt tgtaggtgct
     8761 gtcgatggtg taggcctgcg gacccagctc gacctgcgcc acgtcggaca cccgcacctg
     8821 cgagccgtcc gggttgacct tgagcaggat gttgcggaat tgctcgggcg tgttcagccg
     8881 cgaggggccg atcaccgagg cgttgagctg ctggccgggc acggcaggca gttggcccag
     8941 ctggcccgag gcgatctgta cgttctggtc ctgaatggcc ttggtaacgt cggacgcggt
     9001 gaggccgtag ctcaccagct tggccgggtt gagccagacg cgcatggcgt aggacgagcc
     9061 gaacagctgg aagtcaccca cgccctcggt acggctgatc ggatcctgga tgtaggacgc
     9121 gacgtagtcg cccaagtcgg cgcggttcag cgatcgatca cgcgaaatga aggccgccac
     9181 catcaggaag ttcttggtgg ccttggtgat gcggatgccc tgctgctgga cttccagcgg
     9241 cagcagcggc tgcgccacgg ccagcttgtt ctgcacctgg acctgcgcga tgtcggggtt
     9301 ggtgccctgc gcgaaggtca gcgtgatggt catgctgccg tcgttgttgc tctcggagaa
     9361 gaggtactcg agatggtcga tgccgttgag ctgctgctcg atgatctgca ccaccgtgtc
     9421 ctgcacggtc tgtgccgagg cgcccggata agtcacctgc acggcgatgg ccggcggcgc
     9481 gatattggga tactgggcca ccggcatggt gcggatggcc agcaggcccg ccagcatcag
     9541 cacaatggcg atgacccagg cgaaaaccgg cctgtcaatg aaaaacttag ccatgcgcgg
     9601 gctcccttac tgcttcgctt gctgcgcgga ttggtcgggc gacttcaccg caacgggaac
     9661 gggcggggtc cattcggtcg cggtgacgtc cacgcccggg cgcacgcgct gcaggccttc
     9721 gacgatgacc ttgtcgccgg ccttcaggcc gtcggtcacc agccattggt cgcccacggc
     9781 gcggtcggtc ttgatctggc gcagttcgac cttgttctcg ggcgtcacca ccagggcggt
     9841 gggtatgccg cgcgggctgc gggtcacgcc gcgctgcggc accagcaggc cctgcggcgc
     9901 cacgccgtcg cccaggatgg cgcgcacgaa catgccgggc aggagactgt ggtcgggatt
     9961 ggggaacagg gcgcgcaggg tcaccgaacc ggtgcccgga tcgaccgtga cttccgagaa
    10021 ctgcagcttg ccgggcagcg gatattgcgt gccgtcctcg agcgtgagct tgacatcggc
    10081 ggcctggccg tcgctgctct tgagctggcc gctggccagg gccgacttca ggcgcagcag
    10141 ctgcacgctg gactgggcga cgtcgacata catgggatcg atctgctgga tcgtggcgat
    10201 gggcgaggtc tggttggcgg tgaccagcgc gccctcggtc acggccgagc ggccgatgat
    10261 gccgtcgatg ggcgacagca ccttggtgta gaccaggttg acgcgggcgg tctcgagcgc
    10321 ggccttggcc gacaggacat cggcggcggc ctgctgcgag gcagccacgg cgtcgtcata
    10381 ggcctggcgg ctcacggcgc gggtctcgac cagcggcttg tagcggtcgg ccagcgaggc
    10441 ggccgaccgc tgcgaggcct cggcgcgcgc cagggaagcc ttgctgctgt cgaactgggc
    10501 ctggtagatg gcgggatcga tctggtagag ctgctgctgc gccttgacgc tgccgccctc
    10561 ggtgaacaga cgcttctgca ggatgccgtt gacctggggc cggacttcgg cctcgcggaa
    10621 agcggcggtg cgccccggca gttcggtggt cagggtgacc ggctgggtct tgagcgtgac
    10681 gacccagact tcgggcttgg cagctgcctg ggcagcggag tcctgctgct tgccgcaggc
    10741 ggcgagcccc acggccaagg ccagcgccag caccgacata ccggacgcct tggcgccgcg
    10801 caggatcaga ctttgcttct tcattcggaa ctatccccgg aaagtgacgc gtcgcacgcg
    10861 ccgcctatat acgcaacctg ttgtctatcc atccttcgca atggacgaaa gccgtcggat
    10921 tgttgcaatg caatatacga cctggcaagc cacttcttta cccggggact tacccagaca
    10981 gagataaatg gatacataga ctcaaacttc gaaccttacc gcatttccgt gaccatacct
    11041 atcatggtgt gcaccaggat gaggcgtctc tcggtgcaat gcagcaatca agaaatagat
    11101 tgaaataatt gcccagccgg gcaacttgcc agcaaggata aaacagggca aacagttttt
    11161 tgtcagaaat tttcatcgtc gcgcaggtaa cgccactgcc ccgcgggcag atcccccagc
    11221 gccacccggc cgatgcgcac gcgcttgagg cccaccacct tcaggcccac cagttcgcac
    11281 atgcggcgga tctgccgctt cttgccttcg cgcaatacga agcacaactg gtcggcgttc
    11341 tgccagcgca cctgcgccgg ccgcaggggc ttgccgtcca gggacaggcc gtggttgagc
    11401 aaggccaggc cgctctcgct caggcgtccc tggacgcgca ccaggtactc cttgtcgacg
    11461 tcggaatcct cgccgatgag gtggcgggcg acgcggccgt cctgggtaag gaccaggagg
    11521 ccctgcgaat cgatgtccag ccggccggcg acggccaggc cctgcaggtg ggcgcgcgcg
    11581 aacctgagcg ccgtgcggtc gcccgcatgc cgcgaacgct cgctgatcag ggccaccgcc
    11641 ggggtgtagc ccttttcggc ctggccggag acatagccca cgggcttgtt gagcaggatg
    11701 gtgacgcgcg cggtctggcg cgcctgggcg gcgcgctcca gggtgatgcc ctggtcggga
    11761 taggccctgg cgccgagctc ggagaccacg atgccgtcga ccttgaccca gccgcgctcg
    11821 atataggcgt cggcctcgcg gcgcgagcac aggccgcgct cggacatcag tttggagatg
    11881 cgtacttttt ccataggcgg ctattttaag gggtgggggc cggccatccg ggcacgcgcc
    11941 ggccgggctg ggcataatac ggagccatga cggatctccc ctgccccgcc agccccctgg
    12001 gctggcacgc cgccccgccc ccctccctgc ctgcccggca caaggcctgg ctgtttcgtc
    12061 ccggcgcgct gacggcggga ctgcgcagcc tgggcgccat ggatctgcgg gtgctggccg
    12121 aatacgccgc gggcgtgccg gccgacgagg cccacggcat gcggctggcg ccgggcacgc
    12181 cggtctgggt gcgggagatc gccatggcgg tcgacggccg cgacgccgtc gtggcgcgca
    12241 gcctggcgcc gctggccgcc tcgcacggcg tctggcaagg catgcggcgc ctgcgcacgc
    12301 ggccgctggc ggacatgctc tatcacgacc ggtccatccg gcgctcgccg tttgcctgcg
    12361 cccggctggg ctatccgctg gcccagcacc gcacggtgct gggcgtggac gcgggctacg
    12421 cgcgcgaggc cctgtgggcg cggcgctcgg tgttctggcg cttcggccag ccgctgctgg
    12481 tggcggagtg cttcgtgccg gggttctggc aagacatact gaacgcctga atcacgcgtc
    12541 gcgcaacaac agggcaaacc ctatgcatca gcctcgacta ttgcgtatca acaaatctga
    12601 ttaggcgcgc ctcccccgca ccggtaccat tttttcaaat ggaagacatg gccgggccag
    12661 gggaacagga tgaagtctat agcagtaatc ggtgcaggca ttgtcggcct gtgcaccgcg
    12721 gcgaatctgc agcgcaaggg cctgcgaaca acgctgatcg actcattgcc gccaggcacc
    12781 agcacgacgt acggcaatgc cggcatgatc agcgcgaact ccatcacgcc tgtcgccatg
    12841 cccggcatgt tgcgcaaagt gccgaaatgg ctgcgcgatc ccgaaggacc gctcttcgtc
    12901 gatccgaaat accttccctc ggccttgccc tggctgatgc gctggatacg cgcgggcgcc
    12961 tccatggagc aggttcgccg ctcgtcccaa ggtttgtatc aactgcattc ccacgcgttg
    13021 gacgaataca agaaactgct cggcaccgag catttcgacg ctctggtcaa gacagtcgga
    13081 cagatccacc tctgggatag tccgcaactc cccgacaatg aaaaaatcgc agcctcgatg
    13141 cgcagcgagg ccggcatcga aacgcgcgcc ttgtcgtctg gcgacttgcg cgacatgcta
    13201 cccgagctga cctcttcggt aggctgcgcg ctgtattttc cgaacaacgg ccacaccacc
    13261 agtccattgc gcctggcgca aaccatccat ggcttattca cggaagccgg cggccaaact
    13321 gcgcctgaga aagtactgcg catcgtgccg caaggcagcc aataccgtct ttggacaaat
    13381 tgcggcgatc acagctatga ccaggtagtc gttgcaggcg gagcctggtc gctggccctg
    13441 ctcaagcccc tgggcctgga cttcccgttg gagaccgaac gcggctatca cgtccaggtg
    13501 gatgcgccca atatcgacct gccttatccc attctgcaca aaagccgcgg ctttggtgcc
    13561 tcggtcatgg aagatggatt gcgcctggcc ggcacggttg aaattgccgg tctgcgcaag
    13621 ccgccaaacg cgcggcgcac cgagtccttg ctcaggcacg ccaaagtctt gtttccgaga
    13681 ctggaaatcg ggaagcacaa gatctggatg ggctatcgcc catcgatgcc agactcgcta
    13741 ccggtactgg ctgcaagcag ccgttatccc gggctattta tcgcctgcgg ccatggccat
    13801 acaggcatta ctgcaggcgc aaccaccggc cgcctgatgg cggagatcgt tacccaatca
    13861 cccacgttca tcgatcctgc accgtatcgc ctggaaaggt tttcctagga tgaaccgcgg
    13921 aaaggaagcg cgtccggcaa ccatcgcggt cataggcgcg gggatcatcg gcctgtgcac
    13981 cgcggcgaac ttgcagcgca agggcctgca ggtcacggtg ctcgacccca tgccaccggg
    14041 taccaatgcc acctatggca acggcggcat gatcagcacc gcagccatca caccggttgc
    14101 cttgcccggc atgctgcgca aagtgcccaa atggctgcgc gaccccgaag ggccgctttt
    14161 tgtcgcccca agatatctcc ctgtcgcgct gccctggctg atgcgctggg tgcgcgcggg
    14221 cgccctggat caggtgcgcc atgccgccga ggccttgcac cacctgcatg accatgcaat
    14281 cacggaatat cgacaactgc tcggccccga gcatttcaat tccgtggtca aggtactggg
    14341 gcaagtccac atgtgggaca acaaggagtg gcccgaaagc gagaaaatcg ccgcggcctt
    14401 tcgcgaccaa tacggcatcg cgaccgaaga gctatcgccg gagcgtctgc gcgatatgtt
    14461 gcccgagctc gcgacttcgg tcagccgcgc cttgtacttc cccaataatg ggcacaccac
    14521 caatccactg cggctggcac aagtcatcca tggcttgttc atggatgacg caggccaatc
    14581 gctgcgtgaa aaggtgctgc gcatcacgcc ggaagatggc cgctaccggc tctggacgaa
    14641 ctgtggcgac catatctttg atcaagtcgt cgtagccggc ggagcctggt cgctcgacct
    14701 gctcgcccca ctgggcctcg gctttccgct ggagaccgag cgtggctacc acgttcaaat
    14761 cgccaatccc aagatcgaag tggcctatcc gatcttgcac aaagggcgcg gctttggcgg
    14821 gtcgatgatg gaggatggat tacgcctggg tggcacggta gaaatcgccg gattggacat
    14881 gccgcccaat ccccgccgta ttcaagccat ccagaagcac gccaaagtcg tacagcccag
    14941 gctcgatctt tccgaataca aggtctggat ggggtttcgc ccctcgacac cggattcctt
    15001 gccagtaatg ggagaaagcc gccgccaccc tggcctgcat atcgcctgcg gccacggcca
    15061 tcttggcatc acgggaggcg ccgccagcgg ccgcctgatg gcggaaatca tcaccaaatc
    15121 tcccacgttc attgatccca ccccttatcg cctggaaagg ttcttataag cgaggtcgcc
    15181 catgaagcag caaatcacga aaccacacag tgctacccgt cgcagcatcc ttggcatggc
    15241 gggcgcggcg ctgaccacgc catatttatg gataccccgg gccaatgccg cgggacaagt
    15301 catcatccgt tcgccgggtg gtgtctacga tgacatccgc aaggaaacca tctacgaccc
    15361 gttccgcaag gaaaccggca tcgaagtcat tccagttgcc acaacaggcg caaaactcga
    15421 cgcgatgctt cgctcgggca atatagaact ggacattgtc gacaccggca ttcagctcct
    15481 cgtgtcctat cagaaacagg acgcgttgat acccatcccc tacaaggaat tcaaatacac
    15541 cgatcccgat gatatcgacg aaaactacaa gcatgaatat atcgtcggaa atttcaccta
    15601 tgcacaggcc atggctttca acaccgaggc ctacacgctg gcaacgggcc caaaggactg
    15661 ggcggaattc tgggatgcca aacgctttcc cggaccgcgc atgctggccg atgtcgccac
    15721 aggatcgcct gacctggaag cggccctgat agccgacggc gtgcccatgg acaagctcta
    15781 tccgctggac ctgaaacgcg cattcgattc gctcacgcgc atccggccgt cgattcggaa
    15841 gttctgggac acgggctcgc tgtgcgcgca gatgctgacg aacaaagaag tcgtgatggg
    15901 atcaatctgg agttcccggc tattggcgtc cagagaggcg ggcgcaccgc tggtacagaa
    15961 ctggaatcag catgaggtcc aggtgcaggc ctactccatc ttgaagggcg caccaaatac
    16021 tgaaaacgcc aagaagctga tcgactattg cctgagcaag gacgtgcagc aacgctattt
    16081 cgaacgctac tcggcgggtc cggtcaccaa gacagcctac aagaatctga gcgccgacaa
    16141 acgggaaaac attcctggcg gcgaccgtac ggcgcgctac ggcttcgtcc aggatgccca
    16201 atggtgggga aacctggaaa acaaacaggc agtcagcgct gcctggtcca aatgggtgct
    16261 ctccaagtga agggcgcgga gatcaccctg gaaggcctga ccaagcagta ttacgggcac
    16321 gccgtggtcg aagacgtttc ggccgtcatc ccggccggca gttttttctc tcttctcgga
    16381 cctagcggct ccggcaagac cagcacattg atgatgcttg ccggcttcgt gtcgcccgac
    16441 acgggacgca tcctgctcaa tggacgcgac atcacggcag acacgccaca gcaacgcggc
    16501 ttgggcatgg tattccagaa ctatgccttg ttcccgcatc ttaccgtcga ggagaacgtc
    16561 gccttccctt tgcgcgttcg acgaaaaaaa ccgcaggaga tccgggaaaa agtccgctgg
    16621 gcactggatg tcgtccggct gtccccatac gccgaccgac accccaagca actctcggga
    16681 ggccagcagc aacgcgtggc gctggcccgg gccatagtct tcgaacccgg ggtcgtgctg
    16741 atggacgaac ccctgggcgc cctggacaag aacctgcgct atcacatgca ggtggaaatc
    16801 aaagaaattc agcagcgcct gggcatgacc gtcgtctatg taacgcacga tcaggaggag
    16861 gccatgaaca tgtccgatgg catcgccatc atggaccaag gccgcatcgt gcaaatggac
    16921 agcgctcgcc ggatctacga agacccgcgc agtcaattcg tcgccaaatt tctgggcgag
    16981 gcgaatctga ttccagccga acggctggaa agccacgccg gccgcaaagg agaatccctg
    17041 ttcgtacgcc cggagcgcat acggctggat tcgacaaatg ccgccacacc cgtagattcc
    17101 actcgcgtca acggcatggt cagccgggca tcgttcctcg gcaatatcgt gcgctatagc
    17161 gtcgacctgg gagcaggctg ccagatcacc gcggacatcc acaacaacgg cggagagcgg
    17221 caattttccg ttggcgaagg cgttgccgtc ctgtggcgcc acgccgattc aaaactgctt
    17281 gccgatgact gataccacgc tcgcgcctcg cgctcgttcg cccttgcctt ccagcctgag
    17341 cggcacgggc atgctgttcg tgccgctggc cttgatgctg ctgctgatcc atgtgggccc
    17401 gctgatctgg ttcttcatca agaccttcgc cagcgtccag caaggcttcc tgcagcagat
    17461 cagcgatgtc gtcctctcgg tcgacatgct gcaggtgata tggaccacgc tctggacatc
    17521 gtccctagtg accctgctga cacttttgct ggcctatccc gtggcttata gcctgtgcaa
    17581 ggcagggtcg aaattcacga tggcaggcat cctcgcgtgc atcatcgttc cgtatttcac
    17641 ttccgtcatc gttcggacct attcatggat ggtgctgctc ggccacaacg gtctgatcaa
    17701 tcgcttcttg ctcgatctcg gcttgatcga tgaacctttg cacctgatgt acaaccaggc
    17761 cggcgtgctg ataggcatgg tgtatgtctt gttgccctac ctggttctta cgctgtattc
    17821 gacgatgaaa agcatcgacg acaacctgct gcgcgccgcc agcggcatgg gcgcctcggg
    17881 ggcatacgcc ttctttacgg ttttctggcc gctgagcctg cctggcgtcg tttctggcgg
    17941 cctgattgtc tacatcctgg cgctcggttt cttcattacg ccctccctga tgggcagctc
    18001 caacgatgtc atgatagcca tgctgatcca gcgcgaggtc gaggtggaca tgaactgggg
    18061 actggcagcc gttctgtcgc tggtgctgct cgtcatgacc cttatcctct acatcatcta
    18121 ctgccggtat acgaatatcg atcagatgat gggacggaaa taagggagcc ggccatgaaa
    18181 gccatgacct ggatcaatac attgacgacg ctgctgtgcg tgatattgat cgcaccgatc
    18241 gtgctcattg tcatcctgtc cttcagcggc gatgccatca tgcaattccc cccgcactcg
    18301 ttttcgctgc gttggtatac cgattttttc gccgacacac gatggcgcaa ggcgttgtac
    18361 tcaagcctga ccatcggctt gggagcatgc ctgatcgcga ctttcgtcgg attcctggcg
    18421 gcctatgccc tggtcagagg acagttcaaa gccaagaagc tcatgctgtc gtttgtcctc
    18481 ctgcccttga tcattcccaa tatcatcacg gccattgccc tgtattttct atccgcaaaa
    18541 ttggggctta tcggggccaa gccatggatc agcgtgacgc atgcggtaat aggcttgccc
    18601 atcgtcgtga tgatattgat ttctgccctg caaagcgtgg atgaaaacat cgaacgggcg
    18661 gcctatagcc tcggcgccag ccgcagcatg accttcgtga aggtggtcat ccccctggcc
    18721 ttgcccggtg tcgtctcggc agcgctattc agcttcctga cctcgttcga tgaactcgtc
    18781 atcgccatgt tcctctccgg cctgtcgtcc gaaaccctgc aagtacgcat ctggaacagc
    18841 ctgcaaatgg atgccgaacc catcatcgca tcggtaagcg ccctgctcat cgccgttacg
    18901 gtcatcttgc tcggggtgga tgcgctatta cgtcacaaac gggcacgtcg tgcctaaccc
    18961 tgcttcatgg agaaaactca tgatcgaagt cgttgaaacc ggcattgcaa aatccaaggg
    19021 acctgtctcg ggtgctgtga tcggcacagg tgcgcgcacc ctctacatgg cggtcattcc
    19081 caaagacccg cagagcgggg ccatcgtcaa tggcgacatc gagacccagg cccgccagac
    19141 ttttgccaac atgaagcaga cagtcgaggc ggccggagga acgctgaagg acatggccca
    19201 ggtactgatc tatttgatcg aaggcagcga tgccgcagcc atgaacaagg tgtatggcga
    19261 gttcttcagc gcgccttatc ccaatcgggc aaccgtcgtc gtcaaggagt tgctggcacc
    19321 cggaatgcgc atcgaagtcg ttgcgcatgc ggcactgccc tagcaatagc aagcacaacc
    19381 atccatggga taaccccaat tggaacaaca ttaccccctg ggccagcata gaccctccag
    19441 cctgggggga atacccttga gtcgatgttt gaatttccgg catgtcgagg tcatacgcgc
    19501 agtcgtgctg accggaacag tgacgggtgc cgccaaccga ctgcatgtga cccagccagc
    19561 ggtcagcaat attctgagag atgcggaaga acgactcggt ttcgtattgt tcgaacgagc
    19621 cgggggacgc attaccccta caaaaaaggc tgcgctgcta gtcgccgaga tcgagcgtgc
    19681 gttcaccggc ctggacgcca tcaacgaggt ctgcgggcat ttggttcgga acgaaaaccg
    19741 ccgcatttcg attgcctcca ccccctcctg ggcagcgtcg gtgcttcctt cggttatgcg
    19801 acgctatcgc gatcgcttcc cggatgtgcg gttttccttg ttggcgcgca gttccgacat
    19861 ggtccagtcc atggtgatgt cgcagaaagc cgatatcggc tttggcctgg aagtccccct
    19921 cataccaggc gtgcgccaag agctggtcgt ccggtccttg atccgctgca ttgtcagcga
    19981 gaaccacccc ttggccaaca aacggcaggt agggccgaaa gatctgcacg gccacgacat
    20041 gatcagcttc tctcacctga acgaaggaac cgaggatact tactcagaca tgttcgagca
    20101 ggcctcttct tatccagagt cggtcgtcga ttgcgcaaca agcatcacga cctgcgcatt
    20161 ggtggagagc ggcctgggtt ttgcactagt ccaccaggcg gtactgcatc ttttcaaaca
    20221 cgcccgcgtc cgtgtcatcc cgttcgttcc cactacgact ctggacatgt gcgcctatgt
    20281 ctccgaggca cacgacactt cggcccttaa tctggactat atgatcgagc tggcgcgcga
    20341 acaggcgcat acgctggaaa ccgacatcag ccggctggcc gaagcgcgtg acacaagcaa
    20401 atcgcccctg cgaaggaaag tagcctaagc gaggcggccc accgcgcttt agcgccggga
    20461 cgcgcccggc gcgggcgcag cgcttattgc ttgggcatca gcggcaccag cttgcgcagc
    20521 cgctgctcgg tgtccttcat cgacgcgtcc cactggccgc ccggcagata ggccagcacc
    20581 ggcaggtcgt tggccgccgc cttgcggtag tccggatcct tcaggctggc gctgatcgcc
    20641 gcgttgagcc ggtcgatgat gccggacggc aggcccttgg ggccggccag gccgcgctcg
    20701 gacgtcatca gcacggcata gccctgctcc tgcgcggtcg gcaccgtggg cagggcctgg
    20761 ccgcgctccc cggccggcgc gaattgcgcc acgaccttca ccggattctg ccgcatcatg
    20821 gtggcttccg aggcgctcag gatggcatag tcgatttgcc cgcccatcag cgcggtggag
    20881 gtttccccgc tgcccttgaa cggcacggca atgccctggg tgtgggtcgc ctgctgcaat
    20941 tgcagcaaag tcaggtggcc attggtgccc acgccgttgt tgccgaacga cacctgcccc
    21001 ggcttttgcc gcatggcctg cacgacgccg gccaggtcgg cggcgggatt gtcgtcgcgc
    21061 gcgatgagca cggcggggtc ggccaccagg cgggccacca gcgtcatgcc gtcgggctgg
    21121 tagggcgtgc tctggtacat gggcgtaaac agaaacccgg gcaggttcac cgtgcccagg
    21181 gtgtagccgt ccggcttcga atgctgcaca taggcattcg aaatggcgcc ggtggcgccg
    21241 ggtttgtcga tcaccaggac ctgcgcatga ccgcccaggt agcgggcaag aaaaggcgcc
    21301 aggttgcgcg ccatcaagtc cgtggcgctg ccggcgctat acccgacgat gatctcgatg
    21361 ggacggtcgt cgggccaggc ggcctgcgcg gtattcatgg cggcgcacga cagggccgcc
    21421 gccatcgtca acccatgctt gtatttcatg tcggcctcct atgccgctca ttgcgccgtg
    21481 aagatcttcg gcgcgacctc gtcgaaggtc ttttgaatgc cctgcacttc cttgcccatg
    21541 gcggcgccgc ggacgtcggt gatgctcatg ccgtagtcgt tggtgaactg cttgtagtcg
    21601 tcgctggtga tggccttggc aaacagcgct tccatcttcg ccaggcggtc ttgcggcacg
    21661 cccttgggcg ccaccaggta cgtcacctgc tccaggtcgc cgtaggggaa gacatccaca
    21721 tgatgttcgg cgaacgtcgg cacgtccggc aaggcatcca cgcgctggcg cgcgaacacg
    21781 ccgatcacct tgatcaggcc ggacttgatc tggccgatcg cctccgacgg cttgaccacc
    21841 ccggcatcca gctgcccgcc ggccacgtcg atcagtacct tggagccgcc ggtgtaaggc
    21901 acgttggtgt actggacgtt caccgcctgg gccagcatgg cggcgaaaat ctggttgacg
    21961 ttgttgctgc ccggaatgcc gacggacacg ccggccggct tgtccttcat cagcttgacg
    22021 aaatcctcca cgctgttggc cgggctggat ttcggcacca ccaggatctc cgggtccacg
    22081 ccgaccttgt tgagcacgct gaaatcatcc tgcgtgatct tggtggtctt gagcgcgatg
    22141 gcccccatga gggacggcgt cgccaggccg aaggtatagc cgtccggcgc cgaccgcgcc
    22201 acccggccca ggccgatgat gctggaagcg ccgggaatgt tctcgaccac gatcgtgacg
    22261 ccggccttgc ccagcatctc ggcaagcttg cggccggtca cgtcgatcgc gccgccggca
    22321 ttccagggca cgatcagggt aatggggtgg tcgggatacg cgtcggcggc gcacgcgctg
    22381 gccgtgagaa ggccggcggc cacgccgaac agggctgcaa tgcgatgcaa ggttttcata
    22441 tcatctccta gttatagata tggactgaat gtgggtaacc ggttcagccc ccggccacga
    22501 gggcttgcgg cgaataagcg gatggggtca gcccttgccg ggcgcagaac gccagcagca
    22561 ggcttgcgcc agcccggctc cagggcgccg ggtcgtcacc tccggcatgc cgccgcgccc
    22621 gctcgaacag tgcggcgagc tccccggcca gccagggatg ctcgcgcagc aaatcggact
    22681 tgaccgcgag caggtgattg atgggctggg cctgatggcg cgcgaaccag tcgcgcatga
    22741 cgcggtccgc atccggcacg
//
LOCUS       DASWPO010000297        23418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3105332, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000297 DASWPO010000000
VERSION     DASWPO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 23418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23418
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105332"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1373)
                     /gene="fahA"
                     /locus_tag="VGC69_15615"
     CDS             complement(60..1373)
                     /gene="fahA"
                     /locus_tag="VGC69_15615"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEY0296757.1"
                     /translation="MTLLNETHDPAAKSWVASANQAATDFPIQNLPYAVFRRAGSKEA
                     FRPGVAIGDRIIDLAALAALKPFDGAAAQALAACAGDTLNPLMALGHAHWSALRLALS
                     RALRAGSPLQGKIEPLLVAQAAVEYAMPAHVGDYTDFYISIHHATAVGKQFRPDNPLL
                     PNYKWVPIGYHGRASSLGVNQTFPRPVGQQRPAAEGQAPEFGPCKRLDYELELGIYMG
                     TGNAQGDRIAIDQAESHVFGMSLFNDWSARDLQAWEYQPLGPFLAKNFASTLSPWIVT
                     LEALAPFRATFARPAADPQPLPYLSSAANSAKGAFNVQMQVLLTTEQSRARKAEPAKL
                     SSSNFKDAYWTVAQLVAHHTVNGCNLQPGDLMGSGTLSGPAPAEAGSLLELTDGGKKP
                     LDLPWGEQRTFLQDGDQIIFRAECVKPGYPRIGFGECEGTVVAAR"
     gene            complement(1447..2769)
                     /gene="hmgA"
                     /locus_tag="VGC69_15620"
     CDS             complement(1447..2769)
                     /gene="hmgA"
                     /locus_tag="VGC69_15620"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY0296758.1"
                     /translation="MDTTLQRQSDLQYQSGFGNACATQALPGALPVGQNSPQNCPYGL
                     YAEQLSGTAFTAPRAENRRSWLYRIRPGATHLPFEPANVAPGWLSDFGRAPVTPNQMR
                     WNALPMPGKPTDFIEGMQTWGGNGAPDEQSGVGINLYAANRSMQGRFFYDADGELLIV
                     PQQGRLRLATELGLIDIEPCEIAVIPRGIRFRVELPDGEARGYVLENFGAPLRLPELG
                     PIGSNSLANARDFLTPHAWYEDVSGDFELIGKFTGGFWRARIDHSPLDVVAWHGTHAP
                     YKYDLRRFNAIGSISYDHPDPSIFTVLTSPSDTHGTANMDFAIFPPRILAMEHTFRPP
                     WFHRNVASEFMGLIQGVYDAKAEGFVPGGASLHNCMSGHGPDADTFEKATKADTGKAH
                     YIRDTMAFMFETRRVIRPTEQALNTPLRQADYYECWQGLAKHFDPAKA"
     gene            2961..3866
                     /locus_tag="VGC69_15625"
     CDS             2961..3866
                     /locus_tag="VGC69_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296759.1"
                     /translation="MADLRHLDLNLLVVFQHLLELRNISAVARRLDLSQPAVSNALRR
                     LRDTFGDDLFVRTGQGMLPTPQAERLAGPVAEALGLLDRLLEADEGFDPAASTRRFHI
                     AMTDVGEIHFMPRLMEICAQRAPGVRIDSVRGAGAELRRDMEAGRVDLAVGAFDELDG
                     DIMHRMLFRQGYMTLFRKGHPQAHEGMGLKAFRAQRHLIVSHAAPYGQVNQALEAAGI
                     SLQAHHSVPHFTAVPYVLSTTDLLATVPAKLAASAAPRFDLQMMVPPVRVPALQTNLY
                     WQRRFHRDAGNLWLRGLLVALFAQG"
     gene            complement(4383..5585)
                     /locus_tag="VGC69_15630"
     CDS             complement(4383..5585)
                     /locus_tag="VGC69_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0296760.1"
                     /translation="MPHSDKPSLLIAGAGLGGLTAALALQRRGYRVRVLEQASELREI
                     GAGIQLSANANRVLYRLGLGDAIAAVAAQTAGKRIRLWNSGQTWPLFDLGAQSLERYG
                     YPYHTIYRADLHRVLADAVLAADPQAVVLNARLETVAQDADGVTARMADGSTHRADLL
                     IGADGVHSRVRGGLFGADQAVFSGTVAWRGVIPAGKLPAHLREPYAVNWVGPGAHVIH
                     YPLRRGELINFVGILERDDWQVESWTERGTVAECLNDFKGWHADVQTLIQALDEPFKW
                     ALMLREPLPQWTRGRITLLGDACHPTLPMLASGAAMAIEDGYMLARALDEVADPLQAL
                     QRYEAARVDRTSRVVRGSAENARRFHNPALAHAEGAADYVSREWSEARVHERYEWLFT
                     YNVDDAAI"
     gene            complement(5623..6615)
                     /locus_tag="VGC69_15635"
     CDS             complement(5623..6615)
                     /locus_tag="VGC69_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296761.1"
                     /translation="MMHTRSTPWLRGLAGLMLAGALLGATATALAEGFPSAKPITLVV
                     PFPPGGPTDSSARLFGKVMSEKLGQSVIIENRAGAGGTVGTTYVTRQAPDGYTLLWGG
                     TSSIVVAPALYTNLQYDPIKSFTPIGMAVRGPLILVGRTEFGPKTLPELLQASHAKQL
                     TIASAGTGSIDHLTAELLKSTTGANLLHIPYRGGAPAMADVLGGQVDLLFDTVTLLYP
                     QIAAGKLRAYAVTGATRYNRLPDVPTVAEAIGKPFESYSWFGLMAPAGTPADVVQKLT
                     DALAAGARDPSVIKEMESLGLEPVGGSAQAFALSIHDDLDKWTRIVRQAGIKPD"
     gene            6819..7718
                     /locus_tag="VGC69_15640"
     CDS             6819..7718
                     /locus_tag="VGC69_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296762.1"
                     /translation="MNFSFRQLKAFLAVARYGSFTKAAEQLHITQAGLSAMIRELEDQ
                     AACRLFERTTRVVSLTAAGQRLLPVAERSVHDLSQTLGELGVFAAASADRLKIGVTPL
                     MAAYVIPEALRRFRKRVPDARVEIADVDRVLIQQGVESGELDAGFGAFFSRVSGIRRS
                     AIFPAKLVLAVPDGVGKGPRLARWRDVDPETLIALPPENAIQKMVDKHLGLPPEAAGL
                     PQIVTHLETALALVGAGLGHAVVPSFASVAGRRWRVRLLPIEPAVELDYYCITRSGRP
                     ESDLVIQFTQEFQAVVRALSARH"
     gene            complement(7741..8649)
                     /gene="mutM"
                     /locus_tag="VGC69_15645"
     CDS             complement(7741..8649)
                     /gene="mutM"
                     /locus_tag="VGC69_15645"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY0296763.1"
                     /translation="MPELPEVETTRRGIDPVITGKPLRHLLLHETRMRWPIPSELPAL
                     LAGRTVLECGRRGKYLLLRFEHGVQIVHLGMSGSLRVVIPGAAPAAGLPQGGRAPSGG
                     SKHEEPSVGAPPLRKHDHVEWIFDHATLRLHDPRRFGAVLWHPNGDGPVANHPLLAKL
                     GIEPFDPRFDGRWLHAHFRGRSIAIKQALLAGSAVVGVGNIYCSESLFRAGIDPRTPA
                     QKVSLPRCERLAGAIRATLADALESGGSTLRDYVGASGEPGAYFAIHAAVYERAGQPC
                     KVCGTPIRRMVQGQRATYFCPQCQKR"
     gene            8693..10506
                     /locus_tag="VGC69_15650"
                     /pseudo
     CDS             8693..10506
                     /locus_tag="VGC69_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019912.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            10530..11120
                     /gene="lolB"
                     /locus_tag="VGC69_15655"
     CDS             10530..11120
                     /gene="lolB"
                     /locus_tag="VGC69_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEY0296764.1"
                     /translation="MATRGLARLAGAASLAVLAACATPPQPVAVEAMSADAFARTGRF
                     AITVTPLNEGKTEAVQGGFAWRDDGQHYQLDLTNPLGSIEARVEGQPGAALLTRANGS
                     RLRASDPDALVEDALGSPVPVSGLRDWLRGRLLAGQPVDLKRDAQGRPESFGQDGWRA
                     RLMRYDAQGPRLLVLRREESQRSISVRLVVDAPDQP"
     gene            11157..12149
                     /gene="ispE"
                     /locus_tag="VGC69_15660"
     CDS             11157..12149
                     /gene="ispE"
                     /locus_tag="VGC69_15660"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY0296765.1"
                     /translation="MSLATLYDVPAPAKVNLFLHVVGRRADGYHLLQTVFRFIDLADT
                     LNFERRADGVIERAYELPGVAPEQDLVVRAARALQQATGTAFGARIGLVKRIPQGGGL
                     GGGSSDAATTLIALNRLWNTGLSRRQLMALALPLGADVPVFVFGQPAFAQGVGEDLSA
                     VVLPEAGYLVAQPDISVPTAAIFGAPDLTRDTKPVKIADFLALPSSGFGKNDLEPAAA
                     ARYPEIVRCGMWLEARLRVIESQAMEGRGFQFARQAVRMSGSGACLFVEFATQAQALL
                     AQQEITATMRVAGVLENPSINRQDLPDDNLAPEFRLIQACAGLAEHPLWHWIAS"
     gene            12151..12224
                     /locus_tag="VGC69_15665"
     tRNA            12151..12224
                     /locus_tag="VGC69_15665"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12185..12187,aa:Gln,seq:ttg)
     gene            12368..13318
                     /locus_tag="VGC69_15670"
     CDS             12368..13318
                     /locus_tag="VGC69_15670"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY0296766.1"
                     /translation="MANDSFMIFTGTANSRLAVDVVNHLDMSLGKMTVGRFSDGEVMV
                     EINENVRGKDVFVLQPTCAPTNDNLMEIMVMVDALRRASAGRITAAIPYFGYARQDRR
                     PRSARVAISAKVVANMLQVAGVDRVLTMDLHADQIQGFFDIPVDNIYAAPILLGDIWR
                     RNFSNLVVVSPDIGGVVRARALAKQLEADLAIIDKRRPRANVSEVMNIIGEVDGRTCL
                     IMDDMVDTAGTLVKAAQALKERGAGAVYAYCTHAVLSGGAIDRIEGSELDELVVTDTI
                     PLSEQAAACGKIRQLSSASLLGETILRISNAESVSSLFAD"
     gene            13445..14044
                     /locus_tag="VGC69_15675"
     CDS             13445..14044
                     /locus_tag="VGC69_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY0296767.1"
                     /translation="MKFSATARSVQGSSASRRLRRAGRVPAIVYGGTATPLNIELDHN
                     EIYHAVRKEGFHASILTMEIEGGQSEQVLLRSVQWHPYKQQVLHVDFQRVDAGQALHT
                     KVPLHFINAENSPGVKLSGALISHVITEVEIICLPANLPQFIEVDLGSLLAGGSVHAS
                     DLKLPIGVTLNLHGDNPVVAAAMVKGAGAEEAAGEQPAA"
     gene            14143..14739
                     /gene="pth"
                     /locus_tag="VGC69_15680"
     CDS             14143..14739
                     /gene="pth"
                     /locus_tag="VGC69_15680"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0296768.1"
                     /translation="MSDPIRLIVGLGNPGPEYETTRHNAGFWLADHYADDLRVSFTFE
                     KAFFGMVAKARKGGDTVLLLKPATYMNRSGQSVGALARFYKLAPAQVLVLHDELDLLP
                     GQVKIKQGGGHAGHNGLKDIQAALGSPDFWRIRIGIGHPRTLGLAQQVADFVLHSPRR
                     EEQDGIDSVIDRCRAVLPALLDGDFTQATRQLHGGNPA"
     gene            14745..15506
                     /locus_tag="VGC69_15685"
     CDS             14745..15506
                     /locus_tag="VGC69_15685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY0296769.1"
                     /translation="MDKASRGRLTFRQELADFWHFFRHPTLRRLPGRNASSSLGSDWW
                     PGLRPGRLLAWAIALWVINMALFGPLAVAVADKLGAAHRLDPEHVPVLIAVIWAPMVE
                     EMLFRYSMRRPAQALWLCPLAIVLVLWPVRPWSWVLLVCVLLAACLPLRRGRIARGRW
                     ASDWRREYVRRYGLIYHLVSLVFAAVHLHNFIFDKTTFWLLPFLVLPQWVTGLVLGWM
                     RTRRGIGASMALHALFNGGPVAVILVMLRFLPGSV"
     gene            complement(15445..17322)
                     /locus_tag="VGC69_15690"
     CDS             complement(15445..17322)
                     /locus_tag="VGC69_15690"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0296770.1"
                     /translation="MPLPTDIDLRAQARQLTQEGAGLIEHGRTSEARLVLRQALALQP
                     GLADAHCHLGHALQQIGQVQEALHAYRQALACDAGHLPAHLRLAVLLQELGKQDEAVT
                     AYRHVLDRNPDLPDVHYNLGVLLDDMGRHLDAEVAYRHAIRLRPDHAETHQNLGALLV
                     TLGRLADAEASCRQALALQPQQALAHGCLAAVLALQNQLPQAEAHYRQATVLDAGNAQ
                     THESLGAILHRQNKYAQAETAYRRALALKPADTGTWNNLGTVLDQLNRLPESESAFRQ
                     ALALRPDFADAHFGLALVLLRQGRLAEAWPLYEFRYHPQRQSRLSHWATPPLDCPPWR
                     GEPLAGKSLVIWQEQGHGDVIQFGRYLSQLKALGARRLTLVCMDPLKPLFEQQAAVDA
                     VVAIRKSATLDDVRALLGHDYWTFIMSLPHGLGTTLDTIPPAAYLQPGPAHLERWRTR
                     LAGLAGKKIGLVWKGNAGHSNDHNRSLPGLRTLAPLWEVPGLSFISLQKGQGEDQARV
                     PPPGQALLHLGSDLDDFADSAALVAQLDLLICVDTAVAHVAGALGIPCWVLVPAEGTD
                     WRWMLEDETTPWYPGTMRLFRQAAPGDWAGPVEQLRRALLTRYPARSAASPISPPPAR
                     H"
     gene            complement(17413..18087)
                     /locus_tag="VGC69_15695"
     CDS             complement(17413..18087)
                     /locus_tag="VGC69_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY0296771.1"
                     /translation="MIGNLELICRLLLAALLGSVIGFERERLNWAAGLRTHMLVCVGS
                     TLIMLVSLFGFSDVLGQKDVVLDPSRVAAQVVSGIGFLGAGSILLRGEVVRGLTTAAS
                     LWSVAGVGLAVGGGMYVGAIGATVIVLIILAGIKPIERRFISVKQQRSLRMLAERGSI
                     SLDSVHQALGARSIRVKQFIVQQSEDSQELDDIRIALSRASADEFESICGHLRNMPGV
                     REFRRD"
     gene            complement(18196..19452)
                     /locus_tag="VGC69_15700"
     CDS             complement(18196..19452)
                     /locus_tag="VGC69_15700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0296772.1"
                     /translation="MSGVLLLLIQAFVYFAAMAATFYYRKKIGIGVFFCMLGAMHFLE
                     TYLAAVFFIELPFGLISPGSTILFAGKLAIFLLLYIKEDAESMRQPTYGLLVGNILMV
                     VLALVLRLYTSPASLPGYKPDLSFLDQMGLLMIWGTVLLFIDLISMVVLYERLRTLVT
                     KTIPGRIFVSLAIVLTFDQLFFYAGLRFLTHVPLSALYGGWIAKMGSAAFFSLMLTLY
                     LRYVEGNTPGAPANAPRDVFDRLTYRYRYERLIKQVGRDALTGLQDRGQFDIKGPATL
                     AAAARSRQPVSLLMIDIDRFKEINDRYGHPTGDRAIQDIVATIASAMRTKDDLFRYGG
                     EEFALLCLGMPRDDVMAMAERIRLTVASTPHPDLSQPMTISIGIASFPEHAQNFPTLL
                     ARADEALYQAKSLGRDQVFIWAASPA"
     gene            complement(19612..20850)
                     /locus_tag="VGC69_15705"
     CDS             complement(19612..20850)
                     /locus_tag="VGC69_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEY0296773.1"
                     /translation="MPHSPSRIAVIGGGPAGLAAAEVLAAAGVGVDLFDAMPTLGRKF
                     LMAGRGGLNLTHSEPLALFLTRYGQRQQALAPLVQACPPEALRDWAAGLGVETFVGSS
                     GRVFPREMKAAPLLRAWVHRLRENGVVTHARHRWVGWDTDGRLRFATPDGDTAHAARA
                     VILALGGGSWARLGSDGAWIPLLAERGVAIEALKPANCGFDVAWSDFFRDKCAGEPVK
                     NVQASCMTADGTRLQRRGEFVISGHGVEGSLVYALSAPLRDQLDRAGQALLTLDLLPD
                     HTAGRVLAEVSHPRGARSLSSHLQSRLGIAGVKATLLREVLSKEAMLDAEQLARTIKA
                     LPLALTATRPIDEAISTAGGVRFEALDGNFMLTRLPGVFCAGEMLDWEAPTGGYLLTA
                     CIASGRATARGLLHWLASPV"
     gene            complement(20907..21356)
                     /locus_tag="VGC69_15710"
     CDS             complement(20907..21356)
                     /locus_tag="VGC69_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296774.1"
                     /translation="MQFISDAMHRNSFFSHVFTYVNPSAMEGEPGTFETGQANWKIDD
                     VTTSSKDCSIQYKVEGKSGKLVTLVLGSIEKVAVGTWTKVKGVIKKNGDPDTYAVDVR
                     MKDQNDDSMLEFQDQKTAQRVAKAIDYAAGLCKPPLTAMKTEDDAPY"
     gene            21576..22238
                     /locus_tag="VGC69_15715"
     CDS             21576..22238
                     /locus_tag="VGC69_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY0296775.1"
                     /translation="MTSPKTLSKAPGLRRLMWGGVAGLAIGLLPLPLPILVRLLIAWT
                     GGTLVYLVQAWWVAKTCDASRTRVHAQSQDQPDVLILIVTMAAVCACVAAITMLLLQV
                     RQMSGLERAAYVGLGLAALVTSWLVIHTIYAFHYAHRYYQNEKRGVPNGGGLNFPGQG
                     DPQYSDFLYFSFVIGMTSQVSDVNVTSAGMRILTLVQGVLAFAFNMLVLALSINVVAG
                     AI"
     gene            complement(22255..22773)
                     /locus_tag="VGC69_15720"
     CDS             complement(22255..22773)
                     /locus_tag="VGC69_15720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0296776.1"
                     /translation="MGATPVQQILDGYAEAATPALIARIDSLDAREIYAPVLDLLPAA
                     PVNIADIGAGTGRDAAWFAEQGHHVWAVEPVEALREAGRSRHRSDRITWLDDRLPHLA
                     EARMHGPFGSLRHGPGAPDRPAFPISPQETITASQRLGFTLVRQVGADSVQPGNQTNG
                     VRWTWLALSKSG"
     gene            complement(22802..23362)
                     /locus_tag="VGC69_15725"
     CDS             complement(22802..23362)
                     /locus_tag="VGC69_15725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0296777.1"
                     /translation="MSLNVAVLVGSLRKGSFSRSIALAVKELAAPRLDLGIVEIGDLP
                     LYNPDLDDTAVPSPWTRFRGEVAKADAVLFVTPEYNRSIPGSLKNAIDVGSRPYGKSV
                     WSGKPAAIISVSPGLLGAFGANHHLRQPLVFLNMPILQQPEAYISKVGDLLDADGKLS
                     NDATKGFLKEFAEKFADWIELVKPGR"
BASE COUNT         3698 a         7604 c         8142 g         3974 t
ORIGIN      
        1 cgcgcataac aaaacacccc gccggggcgg ggtgtcggtc gtgccgcggc gcgctcgatt
       61 cagcgggcgg ccaccaccgt gccttcgcat tcgccgaagc cgatgcgcgg gtagccgggc
      121 ttgacgcact cggcgcggaa aatgatctgg tcgccgtcct gcaggaaggt gcgctgctcg
      181 ccccagggca ggtccagcgg cttcttgccg ccatcggtga gttccagcaa tgagccggcc
      241 tcggcgggcg cggggcccga cagcgtgccc gaacccatca ggtcgccggg ctgcagattg
      301 cagccgttga cggtgtggtg cgccaccagc tgggccaccg tccagtaggc gtccttgaaa
      361 ttgctggagg acagcttggc gggttcggcc ttcctggcgc gcgactgctc ggtggtgagc
      421 agcacctgca tctgcacgtt gaaggcgcct ttcgcgctgt tggcggccga gctcaggtag
      481 ggcaggggct gcgggtcggc ggccgggcgc gcgaatgttg cgcggaaggg ggcgagcgct
      541 tcgagcgtga cgatccaggg ggacagcgtg gaggcgaagt tcttggccag gaagggcccc
      601 aggggctggt attcccaggc ctgcaggtcg cgcgccgacc agtcgttgaa caggctcatg
      661 ccgaaaacgt gcgactcggc ctggtcgatg gcgatgcgat cgccctgcgc attgccggta
      721 cccatgtaga tgcccagctc cagctcgtag tccaggcgct tgcagggacc gaactcgggc
      781 gcctggcctt cggcggcggg acgctgctgg ccgaccgggc gggggaaggt ctggttcacg
      841 cccaggctgg aagcgcggcc atgatagccg atgggcaccc acttgtagtt gggcagcagg
      901 gggttgtccg gacggaactg cttgcccacg gcggtggcgt ggtggatgga gatgtagaag
      961 tcggtgtagt cgcccacgtg cgccggcatg gcgtattcca cggcggcctg cgccaccagc
     1021 aggggctcga tcttgccttg caggggcgag cccgcacgca gggcgcgcga cagcgccagg
     1081 cgcagcgcgc tccagtgggc gtggcccagg gccatcaggg ggttgagcgt gtcgccggcg
     1141 caggcggcca gcgcttgggc ggcggcgccg tcgaaaggct tgagggcggc cagcgcggcc
     1201 aggtcgatga tgcggtcgcc tatggccacg ccgggacgga aggcttcttt cgagccggca
     1261 cggcggaaca cggcataggg caggttctga atggggaagt cggtcgcggc ctgattggcg
     1321 ctggccaccc agctcttcgc ggcggggtcg tgggtttcgt tcagcagggt catggcgtgt
     1381 ctctttctaa aaatctaaaa agacgcgagc ccttgcgggc ccgcgtccgg ggatgcttgt
     1441 tgcgtatcag gccttggcag gatcgaagtg cttggcgagg ccttgccagc attcatagta
     1501 atcggcctgg cgcagcggcg tgttcagggc ctgctcggtg ggccggatca cgcggcgcgt
     1561 ctcgaacatg aaggccatgg tgtcgcggat gtagtgcgcc ttgccggtgt cggccttggt
     1621 ggccttctcg aaggtgtcgg cgtcgggacc gtggccgctc atgcagttgt gcaggctggc
     1681 gccgccgggc acgaagcctt cggccttggc gtcgtagacg ccctggatca ggcccatgaa
     1741 ttcgctggcc acgttgcggt ggaaccaggg cggacggaag gtgtgttcca tggccaggat
     1801 gcgcggcggg aagatggcga agtccatgtt ggccgtgccg tgcgtgtccg agggcgaggt
     1861 cagcaccgtg aagatcgagg gatcggggtg gtcgtagctg atcgagccga tcgcgttgaa
     1921 gcggcgcagg tcgtatttgt agggggcgtg cgtgccgtgc caggccacca cgtccagcgg
     1981 cgagtggtcg atcctggcgc gccagaagcc gccggtgaac ttgccgatca gttcgaaatc
     2041 gcccgagacg tcttcgtacc aggcgtgcgg ggtgaggaag tcgcgcgcat tggccaggct
     2101 gttcgagccg atgggcccga gttcgggcag gcgcagcggc gcgccgaaat tctcgagcac
     2161 gtagccgcgt gcctcgccgt cgggcagctc gacccggaag cggatgccgc gcgggatcac
     2221 ggcgatctcg cagggctcga tgtcgatcag gcccagctcg gtggccaggc gcaggcggcc
     2281 ctgctgcggc acgatcagca gctcaccgtc ggcgtcatag aagaagcggc cctgcatcga
     2341 gcggttggcg gcatacagat tgatgccgac cccgctttgc tcgtcgggag cgccattgcc
     2401 gccccaggtc tgcatgccct cgatgaagtc ggtgggcttg ccaggcatgg gcagggcgtt
     2461 ccagcgcatc tggttgggcg tgaccggcgc gcggccgaag tcgctcagcc agcccggcgc
     2521 gacattggcg ggctcgaagg gcaggtgcgt ggcgcccgga cggatgcggt agagccacga
     2581 acggcggttc tcggcgcgcg gcgccgtgaa ggccgtgccc gagagctgtt cggcgtacag
     2641 gccgtagggg cagttttgcg gcgagttctg gccgacaggc agggcgccgg gcagggcttg
     2701 cgtggcgcag gcgttgccga agcccgactg gtattgcagg tcggactggc gttgcagggt
     2761 ggtatccatt gcgtatgtcc tccagggcgc gtggcgcagg ttcgctggca cgggccgtgt
     2821 tgtctctgaa aaattattgt ttcgccaact attgtcgaga atcccccggc atattgctat
     2881 gggaatataa aaatacaggg tataaatttc atgaataata cctggcgcct cgccagccct
     2941 gcccgccata ggaccgcaac atggccgacc tgcgccacct ggacctgaac ctgctggtgg
     3001 tcttccagca cctgcttgaa ctgcgtaaca tctcggccgt ggcgcgccgg ctcgacctga
     3061 gccagccggc ggtcagcaat gccctgcgcc gcctgcgcga caccttcggc gacgacttgt
     3121 tcgtgcgcac cggccagggc atgctgccca cgccgcaggc cgaacgcctg gccggcccgg
     3181 tggccgaggc gctgggcctg ctggaccgcc tgctggaggc cgacgagggg ttcgacccgg
     3241 cggccagcac gcggcgcttc cacatcgcca tgaccgatgt cggcgagatc cacttcatgc
     3301 cgcgcctgat ggaaatctgc gcccagcgcg cgccgggcgt gcgcatcgat tcggtgcgcg
     3361 gcgccggggc cgaactgcgg cgcgacatgg aagccggccg ggtcgacctg gccgtgggcg
     3421 ccttcgacga actggatggc gacatcatgc accgcatgct gtttcggcag ggttacatga
     3481 ccttgttccg caaggggcat ccgcaggcgc acgaaggcat ggggctcaag gccttccgcg
     3541 cccagcggca cctgatcgtg tcgcacgccg cgccctacgg gcaggtgaac caggcgctcg
     3601 aggcggcggg catatcgctg caggcgcacc acagcgtgcc gcatttcacg gccgtgcctt
     3661 acgtgctgag caccaccgac ctgctggcca ccgtgcccgc caagctggct gccagcgccg
     3721 cgccgcgctt cgacctgcag atgatggtcc cgccggtgcg ggtgccggcg ctgcagacca
     3781 atctgtactg gcagcggcgc tttcatcggg atgccggcaa tctgtggttg cgggggttgt
     3841 tggtggcgtt gtttgcgcag ggttgatggg ttgtgtgcgg tgcggggggg ggggggcttg
     3901 tttgggttct tgaggcgtcc gtcgcggcgc ggggccgtgg cggcttcgct gagccgggct
     3961 cgggcgcaac caggcgccct cggcccgctg cgcgggcttc cccgtctcag aggcgccgcc
     4021 acggccccgc gccgcgacgg actcaaggcg tcatgcaagc gcgcccgccg cgccgcaggc
     4081 gttgccgtct tcgatttgag acgatgtgag tctgttgggt ggcaaggcag agggggggta
     4141 tgccggccgg taggcggccg ccgagcgctt ccgaagggag ggcgggggct ttccgggggc
     4201 gtgtttgagc ggagcgcagc gcagcgagtt cgcccccggc ccgcccggac gaggaaggcg
     4261 atggccgagg ccccgtccag gggccgagtc agccgccgtg gccggcatac cccccctctg
     4321 ccttgccacc caacagacgc ctcaagaacc aaaatcatca agcaagcgag ccccacaagc
     4381 aatcagatcg ccgcgtcatc cacgttatac gtgaacagcc attcatagcg ctcgtgcacg
     4441 cgggcttcgc tccactcccg gctcacgtag tccgcggcgc cttccgcatg cgccagcgcc
     4501 gggttgtgga agcgcctggc gttctcggcc gaaccgcgca cgacgcggct ggtgcggtcg
     4561 acgcgggcgg cttcgtagcg ttgcagcgcc tgcagcgggt cggccacttc gtcgagcgcg
     4621 cgcgccagca tgtagccgtc ctcgatggcc atcgccgcgc ccgaggccag catcggcagg
     4681 gtggggtggc aggcatcgcc cagcagcgtg atgcggcccc gggtccattg cggcaagggc
     4741 tcgcgcagca tcagcgccca cttgaacggc tcgtccaggg cctggatcag ggtctgcacg
     4801 tcggcatgcc agcccttgaa atcgttcagg cattcggcca cggtgccgcg ttccgtccag
     4861 gactccacct gccaatcgtc gcgttcgagg atgccgacga aattgatcag ctcgccgcgg
     4921 cgcagcgggt agtggatgac atgggcgccg gggccgaccc agttgacggc atacggctcg
     4981 cgcaggtgcg ccggcagctt gccggcgggg atgacgccgc gccaggccac cgtgcccgag
     5041 aacaccgcct ggtcggcgcc gaacaggccg ccgcgcacgc gcgaatgcac gccgtcggca
     5101 ccgatcagca ggtcggcgcg atgggtcgac ccatcggcca tgcgggccgt gacgccgtcg
     5161 gcatcctgcg cgacggtttc caggcgggcg ttcagcacca cggcttgcgg atccgccgcc
     5221 agcacggcat cggccagcac gcggtgcagg tccgcgcggt agatcgtgtg ataagggtag
     5281 ccgtagcgct ctagcgattg cgcgccgagg tcgaacagcg gccaggtctg gccgctgttc
     5341 cacaggcgga tgcgcttgcc cgcggtctgc gcggccacgg ctgcgatggc gtcacccagg
     5401 cccagccggt agaggacgcg gttggcgttg gcgctgagct ggatgccggc gccgatctcg
     5461 cgcagctcgc tggcctgttc gagcacgcgg acgcggtagc cgcgccgttg cagcgccagg
     5521 gcggcggtca ggccgcccag gccggcgccg gcgatgagca gggaaggttt gtcggaatga
     5581 ggcatggtga tggatgcgtt cgacgcagga aggggctgga gatcagtccg gcttgatgcc
     5641 ggcctggcgg acgatgcggg tccacttgtc caggtcgtcg tggatggata gtgcaaaggc
     5701 ctgcgccgag ccgccgaccg gttccaggcc gagcgattcc atttccttga tcacgctcgg
     5761 gtcgcgggca ccggcggcca gcgcgtcggt cagcttctgc accacgtccg cgggcgtgcc
     5821 ggcaggcgcc atcagcccga accaggagta ggactcgaaa ggcttgccga tggcctcggc
     5881 caccgtgggc acatcgggca ggcggttgta gcgtgtcgcg cccgtgacgg cataggcccg
     5941 cagcttgccg gccgcgatct gcggatacag cagggtcacg gtgtcgaaca gcagatcgac
     6001 ctggccgccc agcacgtcgg ccatggcggg cgccccgccc cgatagggga tgtgcaggag
     6061 attggcgccc gtggtggatt tcagcagttc ggcggtcagg tggtcgatgg accccgtgcc
     6121 cgccgaggcg atggtcagct gcttggcgtg gctggcctgc agcagttcgg gcagcgtctt
     6181 ggggccgaac tcggtgcggc cgaccaggat cagcggcccg cgcacggcca tgccgatggg
     6241 cgtgaacgac ttgatcgggt cgtattgcag attggtgtag agcgcggggg cgaccacgat
     6301 gctgctggtg ccgccccaca gcagggtgta gccgtcgggg gcttgccggg tgacgtaggt
     6361 ggtgccgacc gtgccgcccg cgccggcgcg gttctcgatg atgacggact ggccgagttt
     6421 ttccgacatg accttgccga acaggcgggc gctgctgtcg gtaggcccgc cgggcgggaa
     6481 gggcacgacc agcgtgatcg gcttggccga gggaaagcct tcggccaggg cggtggcggt
     6541 ggcgccgagc agggcgccgg ccagcatcaa gccggcaagg ccgcgcagcc acggcgtgga
     6601 gcgggtatgc atcatgtttg tctcctgttg ccctcgatgc aagcggtgcg gatgcatccg
     6661 ctgcctgggc tgttatggat tcttgaggga aggttggtgc gaaaagcatt tcagcaaatg
     6721 ccgctgaata agtaaaagta gattatttga tagatttata gatatttatt gttaataaaa
     6781 gaacccaccc gaagcctgtt tccgcgaggg catgaccgat gaatttctcg tttcgccaat
     6841 tgaaggcctt cctggcggtg gcgcgttacg gcagcttcac caaggccgcc gagcaactgc
     6901 acatcacgca ggccgggctc agcgccatga tccgcgagct cgaggatcag gcggcctgcc
     6961 gcctgttcga gcgcaccacg cgcgtcgtca gcctgacggc cgcgggccag cggctgctgc
     7021 cggtcgccga acgcagcgtg catgacttgt cgcagaccct gggcgaactg ggggtgtttg
     7081 cggcggcttc ggccgatcgc ctgaagatcg gcgtcacgcc gctgatggct gcctacgtga
     7141 ttcccgaggc gctgcggcgt tttcgcaagc gggtgcccga tgcgcgggtc gagatcgcgg
     7201 acgtggaccg tgtcctgatc cagcagggcg tggaaagcgg cgagctggat gccggcttcg
     7261 gggcgttctt ctcgcgcgtg tcgggcatcc ggcgcagcgc gatcttcccg gcgaagctgg
     7321 tgctggcggt gccggatggc gtgggcaagg ggccccgcct ggcccgctgg cgggatgtcg
     7381 atcccgagac gctgattgcg ctgccgcctg aaaacgccat ccagaagatg gtcgacaagc
     7441 atctcggcct gccgcccgag gcggcggggc tgccgcagat cgtgacgcac ctggagaccg
     7501 cgctggccct ggtcggcgcc ggcttggggc acgccgtggt gccgtcgttt gcctcggtgg
     7561 ccggcaggcg gtggcgggtg cggctgctgc ccatcgaacc ggccgtcgaa ctggactact
     7621 actgcattac ccgcagcggc cgtcccgaat cggacttggt gatccagttc acacaagagt
     7681 tccaggccgt ggtgcgggcc ctgtcggccc ggcattgagg ccctgcccgg cgtccgggcc
     7741 ctagcgcttc tggcactgcg ggcagaaata agtggcgcgc tgcccctgca ccatgcggcg
     7801 gatgggcgtg ccgcatacct tgcagggctg gccggcgcgt tcgtacacgg cggcgtggat
     7861 ggcgaagtag gcgcccggct cgccgctggc gcccacatag tcgcgcaggg tgctgccgcc
     7921 cgattccagg gcgtcggcca gcgtggcgcg gatggccccg gccagccgtt cgcagcgcgg
     7981 cagcgatacc ttctgcgctg gcgtgcgcgg gtcgatgccg gcgcgaaaca ggctttccga
     8041 gcaatagata ttgcccacgc ccaccacggc cgacccggcc agcagggcct gcttgatggc
     8101 tatgctgcgt ccccggaagt gcgcatgcag ccagcggccg tcgaagcgcg ggtcgaaggg
     8161 ctcgatgccc agcttggcca gcagggggtg gtttgccacc ggaccgtcgc cattcgggtg
     8221 ccagagcact gccccgaagc ggcgggggtc atgcagacgt aaggtggcat ggtcgaatat
     8281 ccactccaca tgatcgtgct tgcgcaaagg gggagccccc acgctgggct cttcgtgctt
     8341 gctgcccccc gagggggctc gtccgccttg gggcagcccg gcggcgggcg ccgccccggg
     8401 aattacgacg cgcagcgagc cggacatgcc caggtggacg atctgcaccc cgtgttcgaa
     8461 acgcaacagc aggtattttc cgcggcggcc gcattcgagc accgtgcggc cggccagcag
     8521 ggcgggcagc tcggaaggaa tgggccagcg catgcgcgtt tcgtgcaata gcaggtggcg
     8581 caggggcttg cccgtgatga cgggatcgat tccgcgacgg gtggtttcga cttcgggcag
     8641 ttccggcatg agccagtgta agatgatcca ttggtaactt ggcgattgtg ccatcgccct
     8701 ccaaacccgg ataggctgca tacgttgtcg tactcttttc tcaaagtcgg aactgccgcg
     8761 ctggcgctcg tgctggctgg catgcagccc ctgtcggcct acgccgaaga ccccgcgccg
     8821 gtgccgtccg gccgaggcat ccaactgcgc cagggccaac tgcccctggt gccgctgacg
     8881 ggcgatatct tctaccgcgt gctggcctcc gaaatcgcgg cgcagcgcgg cctgtacggg
     8941 gccgccgccg ccgcgctcat gccgctcgcc cgcgaaaccg gcgatccgcg cctggcgcac
     9001 cgcgcgctcg acttccagct ggccggcggc aatctgcccg gcgcgctcga cgcggcgcac
     9061 ctgtggtcgc agctcgcccc gtatgacaat gacgccggcg cggccgaact ggccctgtcg
     9121 gccgccaacg gccagacccg cggcatggcc ggcgccttgc gccggcgcat cgatcaggcc
     9181 ggggacaagc ccttggccat cgcccaggcc atgggcgtgg tcggccggct cagcgaccac
     9241 cgcctggccc tgcgcatcat ggacgacgcg ctgagcgacc cggccgtgcg caagctgtcg
     9301 gccgcctcgc tggccctggc ggatgccgcc catgcggcgg gcgatgacgc ccgcgccgtc
     9361 atcgaggccc atgccgccct ggtcgccgac cccaagtccg aagccgccgc gcagcgcgcc
     9421 ctggaatacg gcctggccac cgaccgcgac gcggccctgg cgcaggcgcg cgaattcctc
     9481 gcccgcaatc ccaatgcccg ccgcctgcac ttgatgctgg ttgggcagat ggccgagcag
     9541 ggcgactacg atggcgccca ggccgagctc gaagccatgt cgcgccacgc gcccgaggac
     9601 ttcgacctgc tcttcatcca ggcgcaggtg gcgtatcgcg ccggccgcct ggaccaggcc
     9661 cgcaccctgc tcggccaata tctcgacgtg caggagcagc gccagctcag catcgcgccg
     9721 ggggccacca cggctgccgc ctcggcgtcg gacgcccaca tcctgctggc ccgcatcgcc
     9781 gaagaccagg gccgctacga cgaggccgtg gccgagctgg gccgcatcca ggacccgtcc
     9841 atgcgctata ccgcgcagtt gcgccaggcc tcgctgcgcg cccgccaggg ccgcaccgac
     9901 caggcgctgg ccttgctgga cgccgccgcg ccgcaggacg aggacgaacg cctgcagggc
     9961 tatctcgccc gcagccagat ccagcgcgac gccggccgga tcaatgacgc catcgccacg
    10021 ctcgaggcgg ccgaccgcgc cgcgcccgac acctcggaaa tcaagtacga actggccatg
    10081 ctcgacgagc gcatcggccg tctcgacgac gtcgaacgcc tgctgcggca ggtcatcgcg
    10141 ctcgatccgg acaatgcgca ggcctataac gcgctgggct atacgctggc cgaccacaat
    10201 cagcggctgc ccgaggcgct ggacctgatc acccaggcgc tcgatatcgc ccccaacgac
    10261 cccttcatcc tcgacagcat ggggtgggtg cgttttcgca tgggccagaa cgatcgcgcg
    10321 gccgagtacc tgcagcgcgc ctatacgttg cgccccgagg ccgatatctg cgcgcacctg
    10381 gccgaagtgg tctgggccaa gggcgaccac aatcgcgcca ccgagctgct gcgcgagggc
    10441 tacaaaaaag acgccaagaa cgccacgctg ctcgacacgc tcaagcgcct gggagtgcag
    10501 ccttgatgcc atgccgtggg caggttcgcg tggcgacacg cgggctggcc cgcctggctg
    10561 gcgcagcgtc cctggctgtt ctggccgcct gcgccacgcc gccgcagccc gtggcggtag
    10621 aggccatgtc ggccgatgcc ttcgcgcgca ccggccgctt tgccattacg gtgacgccgc
    10681 tcaacgaagg caagaccgag gccgtgcagg gcggctttgc ctggcgcgac gatggccagc
    10741 actatcagct cgacctgacc aatccgctgg gttccatcga ggcgcgtgtc gaaggacagc
    10801 ccggcgcggc cttgttgacg cgcgccaacg gcagccgcct gcgggccagc gaccccgatg
    10861 ccctggtcga ggacgcgctg ggcagcccgg tgccggtcag cggcctgcgc gactggctgc
    10921 gcggccgtct gctggccggg caacccgtcg acctgaagcg tgatgcccag ggccggcccg
    10981 agtctttcgg gcaggacggc tggcgggcgc gcctgatgcg ctacgacgcc cagggcccgc
    11041 ggctgctggt attgcggcgc gaagaatccc agcgcagcat ctcggtgcgc ctggtggtgg
    11101 acgcgcccga ccagccctag gcgggccgca acccgctttt ctcggatacg cacaccatgt
    11161 ccctcgccac gctctacgac gtgccggcgc cggccaaggt caatctcttc ctgcacgtgg
    11221 tgggccgccg cgccgacggc tatcacttgc tgcagaccgt gttccgtttc atcgatctgg
    11281 ccgataccct gaatttcgaa cgccgcgccg acggtgtgat cgagcgcgcc tacgagcttc
    11341 ccggcgtggc gccggagcag gacctggtcg tgcgcgcggc gcgcgccttg cagcaggcca
    11401 cgggcacggc gttcggcgcc cgcatcgggc tggtcaagcg cattccccag ggcggcggcc
    11461 tgggcggcgg ctccagcgat gccgccacca cgctcatcgc gctcaaccgc ctgtggaaca
    11521 cgggcttgtc gcggcgccaa ctgatggcgc tggccctgcc gctgggcgcc gatgtgcccg
    11581 tcttcgtgtt cggccagccg gcattcgccc aaggggtggg cgaagacctg tccgccgtcg
    11641 tcttgcccga ggcgggctac ctggtggcgc agcccgatat cagcgtgccc actgccgcca
    11701 ttttcggtgc tcctgatttg acaagggaca ccaaaccagt caaaatagcg gactttcttg
    11761 ctttacctag ttccgggttt ggaaaaaacg atctggagcc tgcggcagcg gcccgctacc
    11821 ctgagatagt caggtgcggc atgtggctgg aggcgcggct tcgtgtgatt gaaagccagg
    11881 cgatggaagg ccgaggtttt cagtttgcgc gccaagccgt gcgcatgtcc ggatcaggcg
    11941 cctgtctttt cgtcgagttc gccacgcaag cccaggcgct tttggctcag caagaaataa
    12001 ccgctacaat gcgcgtcgct ggcgttttgg aaaacccgtc gataaaccga caggatttgc
    12061 cggacgacaa cctagcgccg gaatttcggt tgatccaggc atgcgccggt ttggctgaac
    12121 atcctttgtg gcactggatt gcgagttagt tggggagtcg ccaagctggt taaggcaccg
    12181 gattttgatt ccggcatgcg aaggttcgaa tccttcctcc ccagccccac cagaacataa
    12241 aaagaagctg ttgaacatgg gatggttcag cagctttttt cgtttccgcc tttccgttgg
    12301 cagttcaggt taggcaaaaa gcttaggcat aagcgtaaaa gccgccaggc aacacgggca
    12361 cagtaccatg gcaaacgata gcttcatgat cttcaccggc acggccaatt cgcgtctggc
    12421 cgtcgatgtg gtcaatcatc tggacatgtc cctgggcaag atgaccgtgg gtcgtttttc
    12481 cgatggcgaa gtcatggtcg agatcaacga aaacgtgcgc ggcaaggacg ttttcgtgct
    12541 gcagcccacc tgcgcgccca ccaacgacaa cctgatggaa atcatggtga tggtcgatgc
    12601 cctgcgccgc gcctcggccg gacgcatcac cgccgccatc ccgtatttcg gctatgcccg
    12661 ccaggaccgc cgcccgcggt cggcccgcgt ggccatctcg gccaaggtcg tggccaacat
    12721 gctgcaggtc gccggcgtcg accgcgtgct caccatggac ctgcacgccg accagatcca
    12781 gggctttttc gacattccgg tcgacaacat ctatgccgcc ccgatcctgc tgggcgacat
    12841 ctggcgccgg aacttctcga acctggtggt ggtgtcgccc gacatcggcg gcgtggtgcg
    12901 cgcccgcgcg ctggccaagc agctcgaggc cgacctggcc atcatcgaca agcgccggcc
    12961 gcgcgccaac gtgtcggaag taatgaacat catcggcgag gtcgacggcc gcacctgcct
    13021 gatcatggac gacatggtcg atacggccgg cacgctggtc aaggccgccc aggcgctcaa
    13081 ggagcgcggc gccggcgccg tgtacgcgta ttgcacgcac gccgtgctgt cgggcggcgc
    13141 catcgaccgc atcgagggct ccgaactcga cgagctggtg gtcaccgaca ccatcccgct
    13201 gtcggagcag gctgcggcct gcggcaagat ccgccagctg tcgtccgcgt cgctgctggg
    13261 cgagacgatc ctgcgcattt ccaacgccga atcggtcagc tcgctgttcg ccgattgaga
    13321 aattcaagaa tttgcccctt gctggtcgcg gtggggggcg gatgaagcca ggcgcatgtc
    13381 gcgcctggtt aacccaggca gtgcaagcgg tatcgcgctg tccattgtta gtttggagtt
    13441 ttccatgaag ttttctgcca ctgcgcgtag cgtccagggt tcgagtgcga gccgccgcct
    13501 gcgccgcgcg ggtcgcgttc ctgccattgt gtatggcggt acggctaccc cgctgaatat
    13561 cgaactcgac cacaacgaga tctaccacgc cgtccgcaag gaagggttcc acgcgtcgat
    13621 cctgacgatg gaaatcgaag gcggccaaag cgagcaggtt ctgctgcgct cggtccaatg
    13681 gcacccgtac aagcagcaag tgctgcacgt cgacttccag cgcgtcgatg ccggccaggc
    13741 cctgcacacc aaggtgcccc tgcacttcat caacgccgag aactcgcctg gcgtcaagct
    13801 gagcggcgcc ctgatctcgc acgtgatcac cgaagtcgaa atcatctgcc tgcccgccaa
    13861 cctgccgcag ttcatcgaag tcgatctggg cagcctgctg gctggcggct cggtgcatgc
    13921 cagcgacctc aagctgccga tcggcgtgac cttgaacctg catggcgaca accccgtggt
    13981 tgccgccgcc atggtcaagg gcgccggcgc cgaagaagct gccggcgaac agcccgctgc
    14041 ctaaatgtgc aagggctctg ccctttgcaa aaaccccgcg cgtgccttgc acccgcgggg
    14101 ttttttgctg gcgggccgca ccccaaggca aaatgagcca ctatgagcga tcccatccgt
    14161 ttgatcgtag gcctgggcaa ccccggaccc gagtacgaaa ccacgcggca caatgccggc
    14221 ttctggctgg ccgatcacta tgccgacgat ctgcgggttt cgttcacttt cgagaaggcc
    14281 ttcttcggca tggtggccaa ggccaggaag ggcggtgaca ccgtgttgct gctcaagccg
    14341 gccacctaca tgaaccgctc gggccagtcc gtgggggcgc tggcgcgctt ctacaagctc
    14401 gcgcccgccc aggtgctggt gctgcacgac gaactcgacc tgctgccggg ccaggtgaag
    14461 atcaagcagg gcggcggcca tgccggccac aacggcctga aagacatcca ggcggccctg
    14521 ggcagcccgg acttctggcg catccgcatc ggcatcgggc atccgcgcac gctggggctg
    14581 gcccagcagg tagccgactt cgtgctgcat tcgccgcgcc gcgaagagca ggacggcatc
    14641 gacagcgtga tcgaccgctg ccgcgccgtg ctgcccgcct tgctcgacgg cgatttcacg
    14701 caggccaccc gccagctgca tggcggcaac ccggcctgac ccccatggac aaggcctcgc
    14761 gcgggcggct caccttccgg caggaactgg ccgacttctg gcatttcttc cggcatccca
    14821 ccctgcgccg cctgccgggc cgcaacgcat ccagcagcct cgggagcgat tggtggccgg
    14881 gcctgcggcc gggccgcctg ctggcctggg ccatcgcgct gtgggtcatc aatatggcgc
    14941 tcttcggccc cctggccgtg gccgtggcgg acaaactggg cgccgcgcac aggctggacc
    15001 cggaacacgt gccggtgctg attgccgtga tctgggcgcc gatggtcgag gaaatgctgt
    15061 ttcgctacag catgcggcgg cccgcgcagg cgctgtggct gtgcccattg gccatcgtgc
    15121 tggtgctgtg gccggtgcgc ccctggagct gggttctgct ggtgtgcgtg ctgctggccg
    15181 cctgcctgcc gctgcgccgc ggccgcatcg cgcgcggccg gtgggccagc gactggcgcc
    15241 gcgaatacgt gcgccgctac ggcctgatct atcacctggt gtcgctggtg ttcgcggccg
    15301 tgcacctgca caatttcatc ttcgacaaga ccaccttctg gctgttgccc ttcctggtgc
    15361 tgccgcagtg ggtcaccggc ctggtgctgg gctggatgcg cacgcggcgc ggcatcggcg
    15421 cctcgatggc gctgcatgcc ttgttcaatg gcgggccggt ggcggtgata ttggtgatgc
    15481 tgcgcttctt gccgggtagc gtgtaagcag ggctcggcgg agttgttcca ccgggcccgc
    15541 ccagtcgccg ggcgcggctt gccggaaaag acgcatggtg ccggggtacc agggcgtcgt
    15601 ctcgtcctcc agcatccagc gccagtcggt gccttcggcc ggcaccagca cccagcacgg
    15661 gatgcccagc gcgccggcca cgtgcgccac ggccgtatcc acgcagatca gcaggtccag
    15721 ctgtgcgacg agcgcggcgc tgtcggcgaa gtcatcgagg tccgacccca ggtgcagcag
    15781 ggcttgtccg ggcggcggca cgcgggcctg atcttcgccc tggcccttct gcaggctgat
    15841 gaagctcagc cccggcactt cccagagcgg ggccagcgtg cgcaggccgg gcaatgaacg
    15901 gttgtggtcg ttcgagtgcc cggcattgcc cttccatacc aggccgatct tcttgccggc
    15961 caggccggcc agcctggttc gccagcgctc cagatgcgcg gggccgggct gcagataggc
    16021 tgcgggcggg atggtgtcga gcgtggttcc caagccatgc ggcaggctca tgatgaaggt
    16081 ccagtagtcg tgccccagca gggcgcgcac atcgtccagc gtggctgact tgcggatggc
    16141 gacgacggca tcgaccgccg cttgctgttc gaacagcggc ttgagcggat ccatgcacac
    16201 cagggtgagg cggcgcgcgc ccagggcctt gagctgcgac aggtagcggc cgaactggat
    16261 cacgtcgccg tggccctgtt cctgccagat caccagcgac ttgcccgcca gcggttcgcc
    16321 gcgccatggc gggcaatcga gcggcggcgt ggcccaatgg ctcaggcggc tttgccgttg
    16381 cgggtgatag cggaactcat acaggggcca ggcctcggcg agcctgccct gccgcagcag
    16441 gacgagcgcc agtccgaagt gcgcatcggc aaagtccggg cgcaaggcca gcgcctggcg
    16501 aaaggccgat tcggactccg gcaggcgatt gagctggtcg agcacggtgc ccagattgtt
    16561 ccaggtgcct gtatcggcgg gcttgagggc cagcgcgcgc cggtaggcgg tttctgcctg
    16621 ggcgtatttg ttctggcgat gcaggatcgc gcccaggctc tcgtgcgttt gggcattgcc
    16681 ggcatccagc acggtggcct ggcgataatg ggcttcggcc tggggcaatt gattctgcag
    16741 ggcaagcacg gccgccaggc agccgtgcgc cagggcctgc tgcggttgca gcgccagcgc
    16801 ctgccggcag cttgcctcgg cgtcggccag gcggcccagg gtcaccagca gggcccccag
    16861 gttttgatgg gtttcggcat ggtcgggccg cagccggatc gcgtgccgat aggccacctc
    16921 ggcgtcgaga tggcggccca tatcgtccag cagcacgccc aggttgtagt gcacgtcggg
    16981 caggtcgggg ttgcggtcca gcacgtgccg gtaggccgtg acggcttcgt cctgcttgcc
    17041 gagttcctgc agcagcacgg ccaggcgcag atgggccggc aggtggccgg catcgcaggc
    17101 cagggcctgc cggtaggcat gcagggcttc ttgaacctgg cctatctgtt gcagcgcatg
    17161 gcccaggtgg caatgcgcat cggccaggcc gggctgcagc gccagggcct ggcgcaggac
    17221 cagccgggct tcgctggtac ggccgtgctc gatgagcccg gcaccttcct gggtcagttg
    17281 gcgcgcctgc gcgcgaaggt cgatgtcggt gggcaatggc atgccagcag tctagccgcg
    17341 gcggctttcg ctcgggtgtc gcccggtttc accgcgtttc accggatcgg gcggatctgg
    17401 cgcaggggcg gttcaatcgc gccggaattc ccgcacgccc ggcatgttcc tgaggtggcc
    17461 gcagatgctc tcgaattcat cggccgaggc gcgcgacagg gcgatgcgta tgtcgtccag
    17521 ttcctgcgag tcttcggact gctgcacgat gaactgcttg acccggatgc tgcgcgcgcc
    17581 caaggcctgg tgcacgctgt cgagcgagat gctgccgcgt tcggccagca tgcgcaggct
    17641 gcgctgctgc ttgacggaga tgaagcggcg ctcgatgggc ttgatgccgg ccaggatgat
    17701 caggacgatg acggtcgcgc cgatggcgcc cacatacatg ccgccgccca cggccaggcc
    17761 gaccccggcc accgaccaca ggctggccgc cgtcgtcagc ccgcgcacca cctcgccgcg
    17821 cagcaggatg gacccggcgc ccaggaaccc gatgccggaa accacctggg ccgccacgcg
    17881 cgaggggtcc agcaccacgt ccttctggcc gagcacgtcc gagaagccga agagcgacac
    17941 cagcatgatc agggtcgagc cgacgcacac cagcatgtgc gtgcgcaggc cggcagccca
    18001 gttcaggcgc tcgcgttcga agccgatgac gctgcccagc agggcggcga ggagcaggcg
    18061 gcagatgagt tcgaggttgc cgatcatcat ggccgctcca tggccgggcg ggtccggcgg
    18121 agcaaatgaa gcaaacttgt catatatccc gcatattgca ccgaaatgcc gatgccgcaa
    18181 agccccgcgg ctcgctcaag cgggggacgc cgcccagatg aaaacctgat cgcgtccgag
    18241 cgacttggcc tggtagagcg cctcgtccgc ccgcgccagc agtgtcggaa aattctgggc
    18301 gtgctcggga aagctcgcga tgccgatgct gatcgtcatg ggctgcgaca ggtccggatg
    18361 cggcgtgctg gccacggtga ggcggatgcg ttccgccatc gccatcacgt cgtctcgcgg
    18421 catgcccagg cagagcagcg caaactcctc gccgccgtag cggaacaggt cgtctttggt
    18481 ccgcatggcc gaggcgatcg tcgcgacgat gtcctggatg gcgcggtccc cggtgggatg
    18541 gccgtagcgg tcgttgatct ccttgaaccg gtcgatgtcg atcatcagca ggctgacggg
    18601 ctgcctggag cgcgcggcgg cagccagcgt ggcgggaccc ttgatgtcga actggccccg
    18661 gtcctgcagg ccggtcagcg cgtcccggcc cacttgtttg atcaggcgct cgtagcggta
    18721 gcgataggtg agccggtcga acacgtcgcg gggggcattt gccggcgcgc cgggcgtgtt
    18781 cccttcgaca tagcgcagat agagcgtcag catgaggctg aagaacgcgg ccgagcccat
    18841 tttggcgatc catccgccgt agagggccga tagcggcaca tgggtgagga aacgcaggcc
    18901 ggcgtagaaa aaaagctggt cgaaggtcag cacgatggcc aggctgacga agatccggcc
    18961 gggaatggtc tttgtgacga gcgtcctcag ccgctcgtag agaaccacca tcgagatcag
    19021 gtcgatgaag agcaggacgg tgccccagat catgagcagg cccatctggt cgaggaagct
    19081 caggtcgggc ttgtagccgg gcaggctggc cgggctggta tagaggcgca ggaccagcgc
    19141 cagcaccacc atcaggatat tgccgaccag caggccatag gtgggctggc gcatgctctc
    19201 ggcgtcttcc tttatataga gcagcaggaa gatggccagc ttgcccgcga agaggatggt
    19261 cgaaccgggc gagatcaggc cgaacggcag ttcgatgaag aacaccgccg cgagataggt
    19321 ctcgaggaag tgcatcgcgc cgagcatgca gaagaagacg ccgatgccta tcttcttgcg
    19381 gtaatagaag gtcgccgcca tggcggcaaa atagacgaag gcctgtatca gcaggaggag
    19441 aacgccggac atatcgcgaa gggttgcttc cgggaagaag gagcgccctg gccagcggtc
    19501 cctttgttat ctgccctatt cagggcgcag gaaaattaac atactttcaa aaatacacat
    19561 attttttgcg accctctctc gcggcgccgt cctgatcggc ggtgaaccgt gctagacggg
    19621 tgaggccagc caatgcagca gccctcgagc cgtcgcccgg ccgctggcaa tgcaggcggt
    19681 cagcaggtag ccgccggtgg gggcctccca gtccagcatc tccccggcgc agaaaacccc
    19741 cggcagtcgg gtcagcatga aattgccgtc gagcgcctcg aagcgcactc cgccggcggt
    19801 gctgatggcc tcgtcgatgg ggcgcgtggc ggtcaatgcc agcggcagcg ccttgatggt
    19861 gcgggccaat tgttcggcgt ccagcatggc ctccttgctc agcacttcgc gcagcagcgt
    19921 ggccttgacg ccggcgatgc cgaggcggct ttgcaggtgc gaggacagcg aacgcgcgcc
    19981 gcgcggatga gacacctcgg ccagcacgcg cccggccgta tggtccggca gcaggtcgag
    20041 ggtgagcagg gcctgccccg cgcggtcgag ctggtcgcgc aagggcgccg acagcgcgta
    20101 gaccagactg ccttcgacgc catgcccgct gatgacgaac tcgccgcgcc gctgcaggcg
    20161 cgtgccgtcg gccgtcatgc agctggcctg tacattcttg accggttcgc ctgcacactt
    20221 gtcgcggaag aagtcgctcc aggccacgtc gaacccgcaa ttggcgggct tgagcgcttc
    20281 gatggccacg ccccgttcgg ccaatagcgg gatccacgcg ccgtcggagc ccagcctggc
    20341 ccagctgccg ccgcccagcg ccaggatcac ggcgcgggct gcatgcgctg tgtcgccgtc
    20401 gggcgtggca aagcgcaggc ggccgtccgt gtcccaaccc acccagcggt gacgcgcgtg
    20461 ggtgaccacg ccgttctcgc gcaggcgatg cacccaggcg cgcagcagcg gcgccgcctt
    20521 catctcgcgc gggaagacgc gcccggacga gccgacgaag gtctccacgc ccagccccgc
    20581 cgcccaatcg cgcagcgcct cgggcggaca ggcctgcacc aagggcgcga gcgcctgctg
    20641 gcgctggccg tagcgcgtca ggaaaagcgc cagcggttcg ctgtgcgtga gattgagccc
    20701 gccgcggccc gccatgagga atttgcggcc cagcgtaggc atggcgtcga agagatcgac
    20761 gcccacgccg gcggcggcca gcacttcggc ggcagccagc ccggcagggc cgccgccgat
    20821 gacggcgatg cgggaagggg agtgtggcat ggaccgcagt gtagaacgta ttgcgttccc
    20881 ggcccgggcg gcgtcatgcc acgccgtcaa tacggggcat cgtcctcggt cttcatggcg
    20941 gtcagcggcg gcttgcagag cccggccgca tagtcgatgg ccttggcgac gcgctgcgcc
    21001 gtcttttgat cctggaactc gagcatcgag tcgtcgttct ggtctttcat ccggacgtcg
    21061 acggcgtagg tgtccgggtc gccgtttttc ttgatcacgc ccttgacctt ggtccaggtg
    21121 ccgaccgcca ctttttctat cgaacccagg accagcgtca ccagtttgcc gctcttgcct
    21181 tcgaccttgt attggatgga gcagtccttg gacgacgtgg tgacgtcatc gattttccag
    21241 tttgcctggc cggtttcgaa agttcccggc tcgccctcca tcgcgctggg attcacataa
    21301 gtgaagacgt gcgagaagaa gctgttcctg tgcatggcgt ccgagatgaa ctgcaaggca
    21361 tcgtcgagcg tgggtttgtg cggcggcggg tcttgcgccc gggcatttca acgcagtgtc
    21421 ctgccgattg aggcccgggg aaagctcctg gaactgatga tagatcttaa gctttgtttc
    21481 atcaggggct aaagagtaac taaaatgttt tgcttcatgc ggttcgatga tctgaagcgc
    21541 atattccgag gcacgaaatt tcgccgtatc cactcatgac atctccgaaa accctttcga
    21601 aagcccctgg cctgcgccgc ctcatgtggg gaggcgtggc cgggctggcg atcggcctgc
    21661 tgcccttgcc gctgccgatc ctggtgcgcc tgctgatcgc ctggaccggc ggcaccctgg
    21721 tgtacctggt gcaggcgtgg tgggtggcaa aaacctgcga tgcgtcccgc acgcgtgtcc
    21781 atgcccagtc ccaggaccag cccgacgtgt tgatcctgat cgtgaccatg gccgccgtct
    21841 gcgcctgcgt ggccgccatc accatgctgc tgctgcaggt caggcagatg agcgggcttg
    21901 aacgcgccgc gtatgtcggc ctgggcctcg cggcgctggt gacctcctgg ctggtgatcc
    21961 atacgatcta cgcattccac tatgcgcatc gctactacca gaacgagaag cgcggcgtgc
    22021 ccaacggcgg cgggctcaac ttccccggcc agggcgatcc gcaatactcc gattttctgt
    22081 atttttcctt cgtgatcggc atgacctcgc aggtttccga cgtgaatgtg acgtccgcgg
    22141 gcatgcggat attgactctg gtgcaggggg tgctggcttt cgccttcaat atgctggtgc
    22201 tcgccttgtc catcaatgtc gtggcgggcg cgatttgaag cgccgcgcct aggttcaacc
    22261 ggacttgctc aaggccagcc aagtccagcg gacaccgttc gtttggtttc ccggctggac
    22321 ggaatcggcg ccaacctgcc gtacaagcgt gaaccccagc ctttgcgatg ccgtgatcgt
    22381 ttcttgcggc gatatcggaa aagccgggcg atccggggcg cccgggccgt gccgcagcga
    22441 cccaaagggg ccgtgcatgc gcgcttcggc aagatgggga agccgatcgt ccagccaggt
    22501 gatccggtcg gagcggtgtc gcgacctacc cgcctcgcgc aatgcctcga ccggctccac
    22561 ggcccacacg tgatgcccct gctcggcaaa ccaggcggcg tccctgccgg tgcccgcgcc
    22621 gatatcggcg atgttgaccg gcgcggccgg caggaggtcg agcacggggg cgtagatttc
    22681 ccttgcgtcc aggctatcga tgcgcgcaat cagcgccggc gttgccgcct ctgcatagcc
    22741 gtcgagaatc tgctggacgg gggtagcgcc catcgggatg cggcgtgcca gtccagtggg
    22801 tttagcggcc gggtttgacc agttcgatcc aatccgcgaa tttctcggca aactctttca
    22861 ggaaaccctt ggtggcgtcg ttgctcagct tgccatcggc gtcgagcagg tcgcccacct
    22921 tggaaatata ggcttccggc tgctgcagga tgggcatgtt gaggaacacc agcggctggc
    22981 gcagatggtg attggcgcca aatgcgccca gcaggccggg ggacacgctg atgatggcgg
    23041 ccggcttgcc gctccatacg cttttcccat aggggcgcga gccgacatcg atcgcgttct
    23101 tcaggctgcc cgggatcgat cggttgtatt ccggcgtgac gaaaagcacg gcatcggctt
    23161 tggcgacttc gccgcggaaa cgcgtccagg gcgacggcac cgcggtgtcg tcgagatccg
    23221 ggttgtagag cggcagatcg ccgatttcga cgatgccgag atccaggcgc ggagcggcaa
    23281 gctccttgac cgcgagcgca atggaccggc tgaaagagcc tttgcggaga ctgccgacaa
    23341 ggactgcgac attgagggac atggtgtttc ctttaaggaa gagtggcttg gaccggggct
    23401 tttcgctgct acgctacg
//
LOCUS       DASWPO010000298        13814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2481279, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000298 DASWPO010000000
VERSION     DASWPO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481279"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..118
                     /locus_tag="VGC69_15730"
     CDS             11..118
                     /locus_tag="VGC69_15730"
                     /inference="COORDINATES: protein motif:HMM:NF025688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 family protein"
                     /protein_id="HEY0296778.1"
                     /translation="MPRRIPSGAVGDTDIYGAQQHAPLLDIDVPLDIAT"
     gene            219..1184
                     /locus_tag="VGC69_15735"
     CDS             219..1184
                     /locus_tag="VGC69_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY0296779.1"
                     /translation="MKFPRALAACIVAGLSLFHGIAAQAAWPGEHPLTLVVPFPPGGT
                     TDMVGRLLASTLGKQLNQTVIVDNRGGAGGTLGAAYAARAKPDGYTLFLATTAHTIAP
                     SIYDTLPYDFSKDFVPITTVASLPHVLIVNAKLPVDSVADLIKYAKAHPGQINFGSAG
                     IGSTDHLSVELFSRSAGIKLEHVPYKGDDPMMTDLMGGQIQLAMPPSGVVVGPVHAHS
                     VKALAVSSAKPSVYFPGIPAIDETAGLSGYTFKTWYALMVQAGTPPDVQARLYQAVVA
                     ALKSPDMQKAVQTINGDPGGEPSKELARLIAEQTQEWHNILKPEK"
     gene            1294..2247
                     /locus_tag="VGC69_15740"
     CDS             1294..2247
                     /locus_tag="VGC69_15740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296780.1"
                     /translation="MYVKALLCALTATVSFAAKADTYPSNPVKVVVPFAAGSSTDLIG
                     REIARVLSEGLHQSFVVENRPGAQSTIGAAEVARAKPDGYTVLIGSTTSIPAAPFLLK
                     QIPYKPLTDLLPVARIGAVPFMLVVRADLPVKTVNDLIDYAHKNPGKLNWGYANAANQ
                     GAAAMMTHTSKMEAVAVPYTSVPQMVIDMLGNRLDFGIIDTTNAASQITAGKLRALAV
                     TSGKPLQQFPDVPPLGKTLPGYELLGWYGVYVPAKTPKPIIDTLAKVLLKGMDSADYK
                     AHLANAGLIPYSAGPEELTRFDESETKKWAEMVKAAGIEPQ"
     gene            2271..3674
                     /locus_tag="VGC69_15745"
     CDS             2271..3674
                     /locus_tag="VGC69_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296781.1"
                     /translation="MHSTSALPAPSANLARALAQFAHGLRHDDLPDDVRHKSRNHILD
                     AIGLAFASQHFPFAAPGLAGFRAAGAPGASTVIGHGSDLQPRDAALSNGYLIHGLDFD
                     DTHPGSIVHPTVACLPAALAVGEMTDATWGELLAAYAAGMETAIRIGRAVKGGFHHTG
                     FHATGIVSHFSSAVVAGKLLNLSVDQIVAAQGIAASTASGVQVFLETGAWTKRMHPGW
                     GALAGITAAQMAKAGFHGPDRPYEGKFALFDTHMQDRAGEVDTESMRLGLGREWTLLD
                     TSIKPYPVCHFIHGCAEAALALRAQLPDLAQVERIVCELPAATLPIVAEPACAKRVPR
                     SDYEAKFSAQFVVAACLLRGRFTLLELEDASLADPRILALAAKVECVAQAETAFPTYF
                     SGAVTVHKTDASSVHHHIPMNLGSGQRALALDDIAVKFRANAALVLTEARTQAVLDTL
                     LNAQADTPVREITRALA"
     gene            3733..5133
                     /locus_tag="VGC69_15750"
     CDS             3733..5133
                     /locus_tag="VGC69_15750"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296782.1"
                     /translation="MSDALPPLLADATRDLARFAAQFRYEDIPADAVERMKTSLLDSI
                     GCCVFGATLPWTRRVQAMVEADQAAPVASLCGAGRKTSIANAVLVNGTAGHAFELDDI
                     HKESIVHPGSIATPIALAYAERDGNWTGRALITAMVAGYEVGTRVGNAATMSLFYRGF
                     HPQGTSGVFVAAGTAAKSLALDAARTQHALGIVGSQAGGLMAAQEGAMVKRFHSGRAA
                     QSGVYAAELASRDFTGIVDVLEASYGGYLSAYSDKPDAARLTDGLGKDWQTAKVGYKP
                     HASVTSIHAALDALSHILRANHLKAADIASIKAGVSHMTYVHCAWEYKAQGVTAAQMN
                     LYYGLAVIAHDGMAFVDQYDEKRLADPLLLDLIGRTEAHVDEEIEGMGPAFRHAATVT
                     VTTRDGRTLTHRILNRRGSPENPISAADVEYKFRNVVRSCLPQASIDRLVELSKRLET
                     LDSVGEIIAIAAAPRA"
     gene            5166..6518
                     /locus_tag="VGC69_15755"
     CDS             5166..6518
                     /locus_tag="VGC69_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296783.1"
                     /translation="MAKTIAEQLASHFAKFDYDSLTPQTRKAVKRLLLDYLGVGVAGS
                     QTGSGKIAREFAASLGGHPQATLIGDGARVPAMQAALANAISSHSVELDDIDVLALFH
                     FSPPVYSAALATAEQVSADGRALLAALAAGCEMMERLSKAANNSLRNRGYHTTPTCGI
                     FGAAIASGRLLGLSEEQIVSSLGLAGAQSGGLMEMYGPSMQKRFNPGPASRNGVTAAA
                     MAKLGFTGAATIFEGERGWLAAFTDENDPGQLTRDLDKPYPLDIEFKPYSCARPIHNA
                     IDCALDIRRRHAPDLSRIKAIGMARHPDWALYHQNTRPVTYHEAQVSLPYSVAVALTD
                     GQALFPQYNNARLKEPMLAKLSDLVEIRVDASLPRGVSCKMTMEMDDGARYMSQVDYP
                     KGSIQNPMDDDELRAKFDSLAVPVLGAARAAEAAAMVADIEQCRDIGQLMRLLAPQGK
                     "
     gene            complement(6495..7451)
                     /locus_tag="VGC69_15760"
     CDS             complement(6495..7451)
                     /locus_tag="VGC69_15760"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEY0296784.1"
                     /translation="MSRIPAVFQKLRLPVIGSPLFIISNPKLLLAQCQAGIVGAVPAL
                     NARPSAQLDDWLAEIRESLPAWDAAHPDRPAAPFAINLIAHKTNARLDEDLAICEKHK
                     VPIVITSLGAREEINQAVHAWGGVTLHDVIHNTFARKAVEKGADGLIAVTAGAGGHAG
                     TRNPFALVQEIRRWFGGPLVLSGSIATGGAILAAQAMGADFAYIGSPFIATEEANASQ
                     AYKQAVVDATSDDIVYSNLFTGVHGNYLAASIRNSGLDPENLPVPERGYEAGAPRAWK
                     DIWGCGQGVGVIDKVVPAADLIARLAREYQAARDSLLSLRRQ"
     gene            complement(7679..8835)
                     /locus_tag="VGC69_15765"
                     /pseudo
     CDS             complement(7679..8835)
                     /locus_tag="VGC69_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
     gene            complement(8873..9667)
                     /locus_tag="VGC69_15770"
     CDS             complement(8873..9667)
                     /locus_tag="VGC69_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0296785.1"
                     /translation="MTRQALLSRREGAVLVLSNNNPQARNALSPEFYRDLAAALGAAA
                     SDPGVGAVVLTGEGGHFCSGGDLRQLATRRALPIEERRARLEGLHDIIRAVRDCPKPV
                     IAAVEGAAAGAGMSLALACDMLVAAKNAVFSLAYVKVGLTPDGGATAFLAECVSRQVL
                     TELCLTGERASGERMHALGVVNRLAEPGQALAQALALAAQVAAGPELATARIKALCRA
                     AYVNTLAQQLEEEAHSMVRSQETEESREGIGAFLAKRPPDFAKLRK"
     gene            complement(9704..10837)
                     /locus_tag="VGC69_15775"
     CDS             complement(9704..10837)
                     /locus_tag="VGC69_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0296786.1"
                     /translation="MNFVHTEDRRMLADTLNRFIAEQYGIETRNQIAYGAEGRSPALY
                     AQFAEIGAIGALFPEEEGGFGGKGFDIGVVFECLGRGLVAEPLLGALVVGQALVQAGT
                     AAQKAALEDIAAGGCIAALAHDEPGSHYELENVSATARKAGDGWVIDGAKGVVGFGER
                     ADLLLVSARTSGKAGEAGGISLFLVPGDAAGLSHRGYNRFEGGRAAELAFEGVKVGAD
                     ALLGKEGAGGAVLERISGYALLALCAEAVGAMDVAKDQTLEYLRTRKQFGVPIGSFQA
                     LQHRMADILLEVEQARSAVINAAAAIDETDRVKRERALSAAKYSIGHIGTLVAQESIQ
                     LHGGIGMTWELPMAHYAKRLVMIDHQFGDEDHHLARFIELGRQ"
     gene            complement(10856..12064)
                     /locus_tag="VGC69_15780"
     CDS             complement(10856..12064)
                     /locus_tag="VGC69_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0296787.1"
                     /translation="MDLQFTPEELAFRDEVRTFLQSKLPAAMAHKVRSNLHLSKQDLE
                     QWHAMLNERGWLANHWPKQYGGPGWTPVEKFIFEHECALAGSPRIVPFGVNMLGPVLI
                     KYGNDAQKDYWLPRILRGEDWWCQGYSEPGAGSDLASVKTTAVRVSDPAGDYYLVNGQ
                     KTWTTLGHHANMIFCLVRTNKEVKPQAGISFLLVDMKTPGVEVRPIITLDGEHEVNEV
                     FFTDVKVPVENLVGEENKGWTYAKYLLTYERTNIAGVGFSVAALEKLKAVADRMRYKG
                     KPLSRDPLFAARMARVEIDLENMKTTNLRVIAAVAGGGVPGAESSMLKIKGTEIRQEI
                     LSLTRRAMGPYAAPFIEQALQAGYGEPPVGPEEAASAAAAYFNYRKLSIFGGSNEIQK
                     NIISKMILGL"
     gene            complement(12204..>13814)
                     /locus_tag="VGC69_15785"
     CDS             complement(12204..>13814)
                     /locus_tag="VGC69_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY0296788.1"
                     /translation="QAAGLVDRLDEGADSVQAGLAYARELLAQQAPVRRTRDLAIADK
                     AAALALLDTLAAETAKKSRGLFSPLKIIECVRHAVELPFDEGLARERALFLQCIDSPQ
                     RAGLVHAFFAERETAKIPEARAAQPRAFNAIAVIGGGTMGAGITVAALDAGLPVTMIE
                     RDAESIARGRANVEKVYDGLIARGRMTPEAKAAVLARYTGSASYDDIGGVDLVIEAVF
                     EDLEVKKAVFKELERVCKPGSVLATNTSYLDIDAIAAVTSRPQDVIGLHFFSPANIMK
                     LLEIVVPAKVSADVVATAFELAKRLKKTPVRAGVCDGFIGNRILAAYRQAADYLMEDG
                     ASPYEIDEAVRAFGYPMGPYQVVDLAGGDIGWASRKRKAPTRDPKARYVEISDRICER
                     GWFGQKTRRGYYLYPEGARAGQPDPEVLAIVDAERAKQGITPRKFTQDEIMRRYMAAM
                     INEGANVVHEGIALRPLDVDVTLLSGYGFPRYRGGPMKYADMVGLDKVLADIREFAQE
                     DALFWKPSPLIEKLVAEGRNFDSLNKAA"
BASE COUNT         2298 a         4880 c         4418 g         2218 t
ORIGIN      
        1 gaaggccacc ttgccgcgcc gcattccgtc cggtgcggtg ggcgacaccg acatttacgg
       61 cgcgcagcag cacgctcccc tgctcgatat cgacgtgcca ctggacatcg cgacctagcc
      121 attcaaaagc actacccgca cagcacgtcc gcaggctcgg gccaacacga gcccgataca
      181 agaaaaaaca acgagatcac acaacggagg agacaaacat gaaatttccg cgcgctttgg
      241 cggcctgcat cgttgcgggc ctgagcttgt tccacggcat cgccgcccag gcggcatggc
      301 ccggggaaca ccccctgacc ctggtcgtgc ccttcccgcc cggcggcacg accgacatgg
      361 tcggccgcct gctcgcctcg acactgggca agcagctgaa ccagaccgtc atcgtcgaca
      421 accgcggcgg cgcgggcggc accctgggcg ccgcctacgc ggcgcgcgcc aagccggacg
      481 gctacacgct ctttctggcc accaccgccc ataccatcgc cccctccatc tacgacacgc
      541 tgccctacga cttcagcaag gatttcgtcc cgatcacgac ggtggcctcg ctgccgcacg
      601 tcctcatcgt caatgccaag ctgcccgtcg attccgtggc cgacctgatc aaatacgcca
      661 aggcgcatcc cggccagatc aacttcggct cggccggcat cggcagcacc gaccaccttt
      721 cggtcgagct cttcagccgc agcgccggca tcaagctcga acacgtgccc tacaagggcg
      781 acgaccccat gatgaccgac ctgatggggg gccagatcca actggcgatg ccgcccagcg
      841 gcgtagtcgt ggggccggtg cacgcccaca gcgtgaaggc gctggccgtg agcagcgcca
      901 agcccagcgt gtacttcccg ggcatccctg ccatcgacga aacggccggg ctgtccggct
      961 acaccttcaa gacctggtac gcgctgatgg ttcaggccgg cacgccgccc gacgtgcagg
     1021 ccaggctcta tcaggccgtg gtcgccgcct tgaaatcgcc cgacatgcaa aaagccgtgc
     1081 agaccatcaa cggcgaccca ggcggagaac cctccaagga actcgcccgc ctcatcgccg
     1141 agcaaacgca agaatggcac aacattttga agcctgaaaa ataaacaggc caggacataa
     1201 aacccccacg ccgcgccttc ggcttgccgc cccccgagga gggggccgtt tttacctcga
     1261 ggcggctcgg cggtaaaaaa caggagacaa aacatgtatg taaaagcact gctctgcgcg
     1321 ctgacggcca ctgtttcgtt cgcagccaag gccgacacct atccatccaa cccggtcaaa
     1381 gtcgtcgttc ccttcgccgc gggatcttcc accgacctga tcggccgcga gatcgcgcgg
     1441 gtgctcagcg aagggctgca ccagtccttc gtggtcgaaa accgtccggg tgcgcaatcc
     1501 accatcggcg ccgccgaagt ggcccgcgcc aagccggacg gctacaccgt gctgatcggc
     1561 agtacgacat cgattcccgc agcgccattt ctcttgaagc aaataccgta caagccgctg
     1621 accgacctgc ttcccgtggc ccgcatcggt gccgtgcctt tcatgctggt ggtcagggcg
     1681 gatctgccgg tcaagacggt gaacgatctg atcgattacg cccacaagaa tcccggcaaa
     1741 ttgaactggg gctacgccaa cgccgccaac cagggcgcgg cggcaatgat gacgcatacc
     1801 tccaagatgg aagcagtcgc agtcccctat accagcgttc cgcagatggt catcgatatg
     1861 ctgggcaatc gcctggactt cggcatcatc gacaccacca atgccgcgtc gcagatcacg
     1921 gcgggcaagc tgcgcgcgct ggcggtgacc agcggcaagc cgctgcagca attccccgac
     1981 gttccgccgc tgggcaagac actgcccggg tatgaactgc tcggatggta cggggtatac
     2041 gtgccggcga agacgcccaa gcccatcatc gataccttgg ccaaggtact gctcaagggt
     2101 atggatagcg ccgattacaa agcccatctg gcaaacgccg gccttattcc ctattcggcc
     2161 ggaccggaag agctgacccg gttcgacgaa agcgaaacca agaagtgggc cgaaatggtg
     2221 aaggcggccg gcatcgaacc gcagtgatcc cagtccaacc gagacccgcc atgcacagca
     2281 cttccgcctt gccggccccg tcggccaacc tggcccgcgc cctggcccag tttgcccacg
     2341 gcctgcgcca cgacgacctg cccgatgacg tcaggcacaa atcccggaac cacatcctgg
     2401 acgcgatcgg cctggccttc gcctcgcagc acttcccctt tgccgccccg ggcctggcgg
     2461 gctttcgcgc cgccggcgcc cccggcgcat cgaccgtgat cggccatggc agcgatctgc
     2521 agccgcgcga cgcggcgctg tccaacggct acctgataca cggcctggat ttcgacgaca
     2581 cgcaccctgg cagcatcgtc caccccaccg tcgcctgcct gccggcggca ttggccgtgg
     2641 gcgaaatgac ggacgccacc tggggcgaac tgctggcggc ctatgccgcc ggcatggaaa
     2701 cggcgatccg catcggccgc gcggtcaagg ggggctttca ccacacgggc ttccatgcca
     2761 cggggatcgt gtcgcacttc agttcggcag tcgtcgccgg caagttgttg aacctgtcgg
     2821 tggaccagat cgtcgccgcg caaggcatcg ccgccagcac ggcttcgggc gttcaggttt
     2881 tcctcgaaac cggcgcctgg accaagcgca tgcacccggg ctggggggca ttggcgggca
     2941 tcaccgccgc gcaaatggcc aaggccggct tccacggccc ggaccgcccc tatgaaggaa
     3001 aattcgcctt gttcgacacg catatgcagg atcgtgccgg cgaggtcgac acggaatcca
     3061 tgcggctggg cctgggccgg gaatggacgc tgctggacac ctccatcaag ccctatccgg
     3121 tctgccactt catccacgga tgcgccgagg cggccctggc gctgcgcgcg cagcttcccg
     3181 atctggccca ggtggaacgc atcgtctgcg agctgcccgc ggccaccctg cccatcgtgg
     3241 ccgagcccgc ctgcgccaag cgggtgcccc gctccgacta cgaagccaag ttcagcgcgc
     3301 agttcgtcgt cgccgcctgc ctgctgcgcg gccgcttcac gctgcttgag ctcgaggacg
     3361 caagcctggc cgatccgcgc atactcgcgc tcgccgccaa ggtggaatgc gtggcccagg
     3421 ccgagacggc ttttccaacc tatttttccg gcgccgtgac cgtccacaag accgacgcga
     3481 gcagcgtgca tcatcacatc ccgatgaacc tgggcagcgg ccagcgcgcc ctggccctgg
     3541 acgacatcgc ggtgaagttc cgcgccaatg ccgccctggt attgaccgag gcgcgcaccc
     3601 aggccgtcct ggacacgctg ctgaacgccc aggcggatac gccggtgcgc gagatcaccc
     3661 gggcgctggc ctgaccggcg ccgtcgctgc ccccatgcca tccctttcac cccgacttgc
     3721 agaggtttgc cgatgagcga cgccctcccc ccgcttctgg cggacgccac ccgtgatctc
     3781 gcccgtttcg ccgcgcaatt ccgttacgag gacatccccg ccgatgccgt cgaacgcatg
     3841 aagaccagcc tgctcgacag catcggctgc tgcgtgttcg gcgcgacctt gccatggacc
     3901 cggcgcgtgc aggccatggt ggaggccgac caggccgcgc cggtcgcctc gctatgcggc
     3961 gccggccgca agacctcgat cgccaacgcg gtcctggtca acggcaccgc cggccacgct
     4021 ttcgagttgg acgacatcca taaagagtcc atcgtccatc ccggttcgat cgccacgccg
     4081 atcgccctgg cctatgccga acgcgacggc aactggacgg gccgcgccct gatcacggcc
     4141 atggtggccg gctacgaagt cggcacccgc gtgggcaacg ccgccaccat gagcctgttc
     4201 taccgcggct ttcacccgca aggcacgtcg ggcgtcttcg tggcggcagg cacggccgcc
     4261 aagtcgctcg cgctggacgc cgcccgcacg caacacgccc tgggcatcgt cggctcgcag
     4321 gccggcggcc tgatggccgc ccaggaaggc gccatggtca agcgctttca cagcggccgc
     4381 gccgcgcaaa gcggcgtgta tgccgccgaa ctggccagcc gcgacttcac cggcatcgtc
     4441 gacgtgctgg aggcgagcta tggcggctat ctcagcgcct actccgacaa gccggacgcg
     4501 gcgcgcctga ccgatggcct gggcaaggat tggcaaaccg ccaaggtcgg ctacaagccg
     4561 catgccagcg tcaccagcat ccacgccgcg ctggacgcgc tctcgcacat cctgcgcgcc
     4621 aaccatctca aggccgccga catcgcctcg atcaaggccg gcgtcagcca catgacctat
     4681 gtgcactgcg cctgggaata caaggcccag ggcgtcaccg ccgcgcagat gaacctgtac
     4741 tacggcctag ccgtgatcgc ccacgacggc atggcctttg tcgaccagta cgacgaaaag
     4801 cgcctggccg acccgctgct gctcgatctc atcgggcgca ccgaggcgca tgtcgatgaa
     4861 gagatcgagg gcatgggccc ggccttccgg cacgccgcca cggtgaccgt gaccacgcgc
     4921 gacgggcgca cgctgacgca ccgcatcctg aaccggcgcg gcagccccga gaacccgatc
     4981 tccgccgccg acgtggaata caagttccgc aacgtggtgc gatcctgcct gccgcaagcc
     5041 agcatcgacc gcctggtcga gctgtccaaa cggctcgaaa cgctggacag cgtcggcgag
     5101 atcatcgcca tcgcggccgc gccgcgcgcc tgaaacctct tccccctacg acaccgcaca
     5161 tcatcatggc caagacgatc gccgaacagc ttgcctccca tttcgcgaag ttcgactacg
     5221 actcgctgac accgcagacc cgcaaggccg tcaagcgcct gctgctcgat tacctgggtg
     5281 tcggcgtggc gggcagccag accggcagcg gcaagatcgc gcgcgagttc gccgcctccc
     5341 tcggcggcca cccgcaggcc acgctgatcg gcgacggcgc gcgcgtgccg gccatgcagg
     5401 ccgcgctggc caatgccatc tcctcgcaca gcgtcgagct ggacgatatc gacgtgctgg
     5461 ccctgttcca cttcagcccc cccgtgtatt cggccgcgct ggccaccgcc gagcaggtct
     5521 cggccgatgg ccgcgccctg ctggcggccc tggcggccgg atgcgaaatg atggagcgct
     5581 tgagcaaggc ggccaacaac tcgctgcgca accgcggcta ccacaccacg ccgacgtgcg
     5641 gcatcttcgg cgccgccatc gcctccggca ggctgctggg cttgtcggaa gaacagatcg
     5701 tgtcgtcgct gggcctggcc ggcgcgcagt ccggcggcct gatggagatg tacggcccat
     5761 ccatgcagaa acgcttcaac cccggcccgg cctcgcgcaa cggcgtaacc gcggcggcca
     5821 tggccaagct gggcttcacc ggcgcggcca ccatcttcga gggcgaacgc ggctggctgg
     5881 cggctttcac cgacgagaac gatcccggcc agctgacgcg ggacctggac aaaccctatc
     5941 cattggatat cgagttcaag ccctattcgt gcgcccgccc gatccacaac gccatcgact
     6001 gtgcgctgga catccgcagg cggcacgcgc ccgacctgag ccgcatcaag gccattggca
     6061 tggcgcgcca ccccgactgg gcgctctacc atcagaacac caggcccgtc acctaccacg
     6121 aggcgcaggt cagcctgccg tattcggtgg cggtggcgct gaccgacggc caggcgctgt
     6181 tcccgcagta caacaacgcg cgcctgaagg aacccatgct ggccaagcta tccgacctgg
     6241 tggagatccg cgtcgacgcc agcctgccgc ggggcgtgtc gtgcaagatg acgatggaga
     6301 tggacgacgg cgcgcgatac atgtcgcagg tcgactaccc caagggctcg atccagaacc
     6361 cgatggacga cgacgaactg cgcgccaagt tcgacagcct ggccgtcccc gtgctgggtg
     6421 cggcgcgcgc ggccgaagcg gccgccatgg tggccgatat cgagcagtgc cgcgatatcg
     6481 gccaattgat gcgcctattg gcgccgcaag gaaagtaggc tgtcccgggc tgcctggtat
     6541 tcgcgcgcca gcctggcgat cagatcggcc gccggcacga ccttgtcgat cacgcccacg
     6601 ccctggccgc atccccagat atccttccac gccctgggcg cgcccgcctc atagccgcgc
     6661 tccggcaccg gcaggttctc cggatcgagc ccggaatttc gtatcgacgc ggccaggtaa
     6721 ttgccgtgca cgccggtgaa cagattgctg tagacgatgt cgtcggacgt cgcgtccacc
     6781 accgcctgct tgtaggcctg cgaggcattg gcttcttccg tggcgatgaa gggcgagccg
     6841 atataggcga aatccgcgcc catggcctgc gccgccagga tggcgccgcc cgttgcgatc
     6901 gaacccgaca ggaccagggg cccgccgaac caccggcgga tctcctgcac cagcgcgaac
     6961 ggattgcgcg tgccggcatg gccgcccgcg ccggcggtga cggcgatcag gccgtcggcg
     7021 cccttctcga cggccttgcg cgcgaaggtg ttgtggatca cgtcgtgcag ggtcaccccg
     7081 ccccaggcgt gcacggcctg gttgatctcc tcgcgcgctc ccaggctggt gatgacgatg
     7141 ggcaccttgt gcttctcgca aatggccagg tcttcgtcca ggcgcgcatt ggtcttgtgc
     7201 gcgatcaggt tgatggcaaa aggcgcggcc ggccgatcgg gatgcgccgc atcccaggcg
     7261 ggcaagctct cccggatctc ggccagccag tcatcgagct gcgcggacgg ccgcgcattg
     7321 agcgccggca cggccccgac aatgccggcc tggcattggg caagcagcaa cttgggattg
     7381 ctgatgatga acagcggcga gccgatcacc ggcagccgaa gcttctggaa tacagcagga
     7441 atcctggaca tacatatcct ttttcaaaca cggaatgact gcaaagcctt gcagcggcca
     7501 cgctgcggcc ccgatcatgc atcgagatgc ggcagccgag cttaagacgc atgagccgag
     7561 gaccccggag aaatccgaag cgcaggcgcc aatggcgccg agcatcggaa tttcgcaggg
     7621 cagcctcggc ggctcaagaa ctcaactcac acatcgcccc aacccacgcc agcacgcctc
     7681 attgcggcaa ggtcagcacg cccttgtcgc gcagggcggc cagctccgca tccgacaggc
     7741 tcagcaggcg ctgcagcaca tcgcgcgtgc cttcgcccag ggtcggcggc gcattgcgga
     7801 tgggcaggcg ctggccgtcc aggcggtaag gcggcgcgaa ggtatgcgtc gtgccggcca
     7861 ccgggtgcgg catctcgcgc agcaggcctc caggcgttcg agcagcgcgc tgcgcggata
     7921 ggtcttgatc aggtccgtca acatggccag caattccacg cggttctgcc cacgctcgac
     7981 gttggtgcgg tagcgcgggt cctccacgat gtcggggcgc tgcagcacct gccggcaaaa
     8041 cttgtcgaac tggacattgt tgcccacggt gatgatgagc gggccgtcgg ccgcctcgaa
     8101 catgccgtag ggcacgatgg aggggtgggc attgccgtag cgcttggggt cgtggcccag
     8161 cagcatggca tccagcgcgt aatagctgct gatcatgatg ccgcagtcgt agagcgccag
     8221 ctcgatcagg cgccccctgc ccgtgcgctc gcggcggaac agggcggcca gcacggcctg
     8281 cgcggcatac atgcccgtca tcatgtcgac gaccgccacg ccgaacttga gcggcggctg
     8341 cgcggcctcg ccgttgatgg ccatgagccc cgtctccccc tggatcacca ggtcgtagcc
     8401 gggccggtcg gcctgcgggc cggacgtatc gtagccggcg atcgcgcaat agacgagatc
     8461 gggcttgatg gccttgagct gctcgtagcc cacgcccagc ttctgggcgc cgtcgtgcct
     8521 gaaattgtgg atcaccacgt cgaactgcgg cagcatttca tggatcaggc acatgccctc
     8581 cggggagcgc aggtccagcg tgacggagcg cttgttgcgg ttcatggccg agtaatacgt
     8641 ggtctcggtc ttgccgatgc gcatgcccca atcgcgcgtg tcgtcgccgt ggccggggtg
     8701 ctccaccttg atgacctcgg cgccgaagtc ggccagcacc tgcccgcaca ggggcccggc
     8761 gaacacgcgg gaaagatcca gcacccgcac gccttccagc ggaaagtcca ggccgccggt
     8821 cgtggtcgtg ttcattgttt tcggtctcct cgcttgtttc gttcgtctag ttctatttgc
     8881 gcagcttcgc gaaatccggc ggccgctttg ccaggaaggc gccgatgccc tcgcgcgact
     8941 cctcggtctc ctgcgagcgc accatggaat gggcttcctc ttcgagctgc tgcgccagcg
     9001 tattcacgta ggccgcgcgg cacagcgcct tgatgcgggc cgtggccagc tcgggcccgg
     9061 cggccacttg cgccgccagg gccagcgcct gcgccaaggc ctggcccggc tcggccaggc
     9121 ggttcaccac acccagggcg tgcatgcgtt cgccgctggc gcgctcgccc gtcaggcaca
     9181 gctcggtcag cacctggcgc gacacgcact cggccaggaa cgccgtggcg ccgccgtcgg
     9241 gcgtgagccc gaccttgaca taggccaggg aaaacacggc attcttggcc gccaccagca
     9301 tgtcgcaggc cagcgccagc gacatgccgg ccccggcggc cgcgccctcg accgccgcga
     9361 tcacaggctt ggggcagtcc cgcaccgcgc ggatgatgtc atgcaagccc tcgagcctgg
     9421 cccggcgctc ctcgatgggc agcgcgcggc gcgtggcgag ctggcgcagg tcgccgcccg
     9481 agcagaaatg gccgccctcg ccggtcagca ccacggcccc cacgcccgga tcggacgccg
     9541 ccgcgccgag cgcggcggcg aggtcccggt agaactcggg cgacagcgcg ttgcgcgcct
     9601 gcgggttgtt gttgctcaac accagcacgg cgccttcgcg gcgggacagc agggcttgtc
     9661 gtgtcatggt cgtggcgcct tcatgttctc ggtggatgcg gctttactgc cggcccagtt
     9721 caatgaagcg ggccaggtga tggtcctcgt cgccgaactg gtggtcgatc atgaccaggc
     9781 gcttggcgta gtgcgccatg ggcagttccc aggtcatgcc gatgccgcca tgcagctgga
     9841 tgctttcctg cgccaccagc gtgccgatat ggccgatgct gtacttggcc gccgacaggg
     9901 cgcgctcgcg cttgacgcga tcggtctcgt cgatggccgc cgccgcattg atgacggccg
     9961 aacgcgcctg ctcgacttcc agcaggatgt cggccatgcg atgctgcagc gcctggaagc
    10021 tgccgatggg cacgccgaac tgcttgcggg tgcgcaggta ttccagcgtc tggtccttgg
    10081 ccacgtccat cgcgcccacg gcctcggcgc acaaggccag cagggcatag ccgctgatgc
    10141 gctccagcac ggcgccgcct gcgccttcct tgcccagcag ggcatcggcg ccgaccttca
    10201 cgccctcgaa cgccagctcg gcggcccggc cgccctcgaa gcggttgtag ccgcgatggg
    10261 acaagcccgc cgcatcgccc ggcaccagga acaggctgat gccgcccgcc tcgcccgcct
    10321 tgcccgacgt gcgcgcggac accagcagca ggtcggcccg ctcgccgaaa cccaccacgc
    10381 ccttggcgcc gtcgatcacc cagccgtcgc ccgctttgcg cgcggtggcc gagacgttct
    10441 ccagttcgta atggctgccc ggctcgtcat gggccagggc cgcgatgcag ccgcccgccg
    10501 cgatgtcttc cagcgcggcc ttctgcgccg ccgtgcccgc ctggaccagc gcctggccga
    10561 cgaccagcgc ccccagcagg ggctcggcca ccaggccgcg gcccaggcat tcgaacacca
    10621 cgccgatgtc gaagcccttg ccgccgaacc cgccctcttc ctcggggaac agggcgccga
    10681 tggcgccgat ctcggcaaac tgcgcgtaca gcgcggggct gcggccctcg gcgccgtagg
    10741 cgatctggtt gcgggtctcg atgccgtact gctcggcaat gaagcgattc agcgtgtccg
    10801 ccaacatgcg gcggtcttcg gtgtgcacaa agttcatggc gtcgcttccc ttgccttaca
    10861 ggcccaggat catcttggag atgatgtttt tctggatttc gttggagccg ccgaagatgg
    10921 acagcttgcg atagttgaaa tacgccgcgg cggccgacgc ggcctcttcg ggacccaccg
    10981 gcggctcgcc atagcccgct tgcagggcct gctcgataaa gggcgcggcg taggggccca
    11041 tggcgcggcg cgtcagcgac aggatctcct ggcggatctc cgtgcccttg atcttgagca
    11101 tggagctttc cgcgccgggc acgccgccgc cggccaccgc ggcgatcacg cgcaggttgg
    11161 tggtcttcat gttttccagg tcgatctcga cccgggccat gcgggccgcg aacagcgggt
    11221 cccgcgacag gggcttgccc ttgtaccgca tccgatcggc cacggccttg agtttttcca
    11281 gcgccgccac cgagaagccc acgccggcga tattggtgcg ttcgtaggtc agcaggtact
    11341 tggcgtaggt ccagcccttg ttctcctcgc caaccaggtt ctccacgggc accttcacgt
    11401 cggtgaagaa cacctcgttg acctcgtgtt cgccgtcgag cgtgatgatg gggcgcactt
    11461 ccacgccggg tgtcttcatg tcgaccagca ggaagctgat gcccgcctgg ggtttgactt
    11521 ccttgttggt gcgcaccagg cagaagatca tgttggcgtg gtggcccagg gtggtccagg
    11581 tcttctggcc gttgacgagg tagtaatccc ccgccgggtc cgaaacccgc acggccgtgg
    11641 tcttgaccga ggccaggtcg gagccggcgc cgggctcgga atagccctgg caccaccagt
    11701 cctcgcccct gaggatgcgc ggcagccagt agtccttctg cgcatcgttg ccgtacttga
    11761 tgagcaccgg gcccagcatg ttcacgccaa agggcacgat gcgcggcgag cccgccaggg
    11821 cgcattcgtg ctcgaaaatg aatttctcga ccggggtcca gcccgggccg ccgtactgtt
    11881 tgggccaatg gttggccagc cagccgcgct cgttgagcat ggcatgccac tgctccaggt
    11941 cctgcttgga cagatgcagg ttgctcctga ccttgtgcgc catcgcggcg ggcagtttgc
    12001 tttgcaggaa agtccggact tcgtcgcgga aagccagttc ttctggcgtg aattgcagat
    12061 ccatcttgtc ctcgggaatt ttcaggcagc ctcgccgcag cgacgtggaa ctcgccgcgc
    12121 acgcaatggc ggctgcacgc ataacgcatg cctcgcatgc attgttgggg cctcgcggcc
    12181 gttcccggcc gcgcgggtcc cgctcaggcg gccttgttca ggctgtcgaa attcctgcct
    12241 tcggccacca gcttctcgat gagcggcgac ggcttccaga acagggcgtc ttcctgggcg
    12301 aactcgcgga tgtcggccag caccttgtcc aggcccacca tgtcggcata cttcatgggg
    12361 ccgcctcgat aacgcggaaa accgtagccc gacagcaggg tgacgtccac gtccagcggc
    12421 cgcagggcga tgccctcgtg cacgacattg gcgccctcgt tgatcatggc cgccatgtag
    12481 cggcgcatga tctcgtcctg cgtgaacttg cgcggcgtga tgccctgctt cgcgcgctcg
    12541 gcgtcgacga tggccaatac ctccgggtcg ggctggcccg cgcgggcgcc ttcggggtac
    12601 aggtaatagc cgcgccgggt cttctggccg aaccagccgc gctcgcagat gcgatcggaa
    12661 atctccacgt agcgggcctt ggggtcgcgc gtgggcgcct tgcgcttgcg cgaggcccag
    12721 ccgatgtcgc cgccggccag gtcgaccact tgataaggcc ccatgggata gccgaaggcg
    12781 cgcacggcct cgtcgatctc gtagggcgaa gcgccgtctt ccatgaggta gtccgcggcc
    12841 tggcggtacg cggccaggat gcggttgccg atgaagccgt cgcacacgcc ggcgcgcacc
    12901 ggcgtcttct tgagccgctt ggccagttcg aaggccgtgg cgaccacatc ggcggacacc
    12961 ttggcgggca ccacgatctc cagcagcttc atgatgttgg ccgggctgaa gaagtgcagg
    13021 ccgatcacgt cctgcggccg cgacgtgacg gcggcaatgg cgtcgatgtc caggtacgac
    13081 gtgttggtgg ccagaacgga acccggcttg cacacgcgct cgagctcctt gaaaacggcc
    13141 ttcttcactt ccaggtcctc gaacaccgcc tcgatcacca ggtcgacgcc gccgatgtcg
    13201 tcgtaggaag cgctgcccgt gtagcgggcc aggacggccg ccttggcctc gggcgtcatg
    13261 cggccgcgcg cgatgaggcc gtcgtagacc ttctccacat tggcgcggcc gcgcgcgatg
    13321 gattcggcat cgcgctcgat catggtcacg ggcaggccgg catccagggc cgcgaccgtg
    13381 atgcccgcgc ccatggtgcc gccgccgatc acggcaatgg cgttgaacgc gcgcggctgg
    13441 gcggcacggg cctcgggaat cttggcggtc tcgcgctcgg cgaagaaggc atgcaccagc
    13501 ccggcgcgct gcgggctgtc gatgcattgc aggaacaggg cgcgctcgcg ggccaggcct
    13561 tcgtcgaacg gcagctcgac ggcatggcgc acgcactcga tgatcttcag gggcgagaac
    13621 aggccgcgcg acttcttggc ggtctcggcg gccagcgtgt ccagcagggc cagcgcggcg
    13681 gccttgtcgg cgatggccag gtcgcgcgtg cggcgcacgg gcgcctgttg cgccagcagt
    13741 tcgcgggcat aggccaggcc ggcctggacc gagtccgcgc cctcgtccag gcggtcgacc
    13801 aggccggcgg cctg
//
LOCUS       DASWPO010000299        10689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_339119, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000299 DASWPO010000000
VERSION     DASWPO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339119"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1107
                     /locus_tag="VGC69_15790"
     CDS             163..1107
                     /locus_tag="VGC69_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEY0296789.1"
                     /translation="MNAPADRMHYERLIAQAQTLPATACAVAHPCDETSLGVAVEAAR
                     LGLIQPILVGPQAKIRGTAEQFGLSLDGLEIVDAPHSHAAAAAAVALVRNGRASLLMK
                     GSLHTDELMHEVVARDTGLRTGRRISHVFIMDVPTYPEPLFITDAAVNIFPDLATKAD
                     IVRNVIDLHIGLGLGEPRVAILSAVEQVNPDIPGTIDAAALCKMADRGQITGGVLDGP
                     LALDNAINEEAARIKGIKSPVAGRAQVLVVPDLEAGNMLAKNLTFLSNAEAAGIVLGA
                     RVPIILTSRADSPRTRLASCAVAAIYADHLARTRPVAS"
     gene            1162..2346
                     /locus_tag="VGC69_15795"
     CDS             1162..2346
                     /locus_tag="VGC69_15795"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY0296790.1"
                     /translation="MRDTILVINAGSSSIKFYLYDITGPGQLQARLGGQLSGIGTPHP
                     QLSVSSAEGAVEVERQIAPAHAADVSNAQDVVGTWLSGHLNGVPRAVGHRVVHGGAEF
                     TGPVLVDDAVLAKLDTYTDLAPLHQPHNLGPIRVIRERRPDLPQVACFDTAFHRSHGD
                     LADRFAIPESLYARGVRRYGFHGLSYEYIAQRLKAVLPELAAGKVVAAHLGSGVSACA
                     MQAGHSVDSTMGFTAMDGLPMSTRPGRLDAGVVLWMMEQGMDHDAIEALLYHDCGLKG
                     LSGISGDMRDLQASGDPRARLALDYFAWRSAEGILGLGCAMNGIDGIVFTAGIGEHSA
                     EMRAAIAAHWQWLGVKLDPERNARHEQRISADDSRIGLYVVPTNEELIIAQHTLDLVS
                     RA"
     gene            2349..3140
                     /gene="fabI"
                     /locus_tag="VGC69_15800"
     CDS             2349..3140
                     /gene="fabI"
                     /locus_tag="VGC69_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY0296791.1"
                     /translation="MTPTPAPPPALPLAGRRGLVTGIANENSIAWGCARAFRALGAEV
                     AVTYLNDKTHAYVEPLARQIEAPLLMPLDLLQSGQLEAVFDQLRSHWGSLDFVLHSLA
                     YAPREDLHGRVADCSRDGFLQAMDVSCWSFIRMAKLAEPMMQHGGALFCMSYYGAEKV
                     VEHYNMMGPVKAALEAATRYLAAELGPQGIRVHAISPGPLKTRAASGLDHFDDLLDQA
                     QAKAPTRSLVSIEDVGEATAWLATDAARRITGQTLYIDGGYHIID"
     gene            complement(3146..4729)
                     /locus_tag="VGC69_15805"
     CDS             complement(3146..4729)
                     /locus_tag="VGC69_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0296792.1"
                     /translation="MSQTLVSPVAAPPARSRYLGLAVFFLFLIVGVGYVVTHIAADLE
                     PVSMPSSLPYILLGVALLIALGFEFVNGFHDTANAVATVIYTRSMAPNLAVVWSGLWN
                     FLGVLTSTGVVAFGILQLLPVELILQVGSSAGFAMVFALLIAAIIWNLGTWYFGLPSS
                     SSHTLIGSIIGVGLMNQMLHGPSGTSGVDWDQALGVGKALLFSPVVGFVLSAVLLLAL
                     KALVKVPALYKEPAGNAPPPFWIRGLLVLTCTGVSFAHGSNDGQKGMGLIMLILIGTV
                     PTAYALNKAVTPAETHTFVAVAQQASATFAKYEKGVISAQPREDVESFVQTRQLAPAT
                     LSALQQLTDGIAGDVQASGSMAQVGQGQAKNIRNDMYLVSEGIRLVGKSADNPIAAQD
                     QGVVDNYRKSLDHATKFIPTWVKVAVAIALGLGTMVGWKRIVVTVGEKIGKQHLTYGQ
                     GASAELVAMLTIGAADVYGLPVSTTHVLSSGVAGTMAANGSGLQWGTVRSLVMAWVLT
                     LPVSIALAAGLYWLFRGLF"
     gene            4972..6003
                     /locus_tag="VGC69_15810"
     CDS             4972..6003
                     /locus_tag="VGC69_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY0296793.1"
                     /translation="MTLALTPATFDADISGLLYAFRFQPGQPGQELDSASAQDALGQL
                     DESHGGPTQDFLWLHFNLSQAGCTHWLQQHLNIPDEFFEMLGEQVHSTRLERQDDALV
                     AVFNDVIFELDHTPSDVTTSWVYAHRNLMISMRYKPLRSIDRLRMSIRRGETFESTAD
                     LLSHLMREQADLMVEIVRRANTEVDRVEDRFLAADTAGNRQTLSSNRRVLVRLQRMLA
                     PEPGSVFRLLARPPYWLTQDDVQSLRESTEEFSVVLGDMTGLIERIKLLQEEIAANLQ
                     EQNNRTLFVLTLVTVLALPINMVAGLFGMNVGGIPLASDPNGFWFLVLCVIAFTGLLA
                     WRSLRRANR"
     gene            complement(6007..7200)
                     /locus_tag="VGC69_15815"
     CDS             complement(6007..7200)
                     /locus_tag="VGC69_15815"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0296794.1"
                     /translation="MSKVQTLTALAKSGALSGKRVFIRADLNVPFDDAGNISEDTRIR
                     ASVPGIALALEAGAAVMVTSHLGRPTEGTLGPGDTLAPVGRRLAELLGKPVKLVQNWV
                     DGVAVAPGEVVLLENCRVNQGEKKDDETLSRKMAALCDVYVNDAFGTAHRAEATTHGM
                     ARFAPIACAGPLLAAELDALGRALHEPKRPLVAIVGCSKVSTKLSILQSLAGKVDQLV
                     VGGGIANTFLLACGLPIGKSLAEPDQVEQARAVIEMMQQRGAAVPIPVDVVCGKQFSP
                     DAAAVTKAAGEVEADDMIFDLGPKTAQGLAEIIARAGTIVWNGPVGVFEFDQFAGGTE
                     TVSKAIACAAGFSIAGGGDTLAAIAKYGIGEQVDYISTGGGAFLEFLEGKALPAVAIL
                     QARAA"
     gene            complement(7386..8396)
                     /gene="gap"
                     /locus_tag="VGC69_15820"
     CDS             complement(7386..8396)
                     /gene="gap"
                     /locus_tag="VGC69_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY0296795.1"
                     /translation="MTIRVAINGYGRIGRNVLRAHYESGKKHDLEIVAINDLGDPKTN
                     AHLTQYDTAHGRFPGEVSVDGDYMVVNGDKIRVLANRNPAELPWGELGVDVVMECTGF
                     FTSKEKASAHLRGGAKKVLISAPGGKDVDATIVYGVNQNVLTSAMTVVSNASCTTNCL
                     APLVKPLNDKLGLESGLMTTVHSYTNDQVLTDVYHEDLRRARSATQNMIPTKTGAAAA
                     VGLVLPELNGKLDGFAIRVPTINVSIVDLSFIAKRDTTVEEVNAILKAASEGELKGIL
                     AYNTAPLVSSDFNHNPASSTFDATLTKVSGRLVKVTSWYDNEWGFSNRMLDTAGAMMA
                     AK"
     gene            complement(8432..10474)
                     /gene="tkt"
                     /locus_tag="VGC69_15825"
     CDS             complement(8432..10474)
                     /gene="tkt"
                     /locus_tag="VGC69_15825"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0296796.1"
                     /translation="MSLPTPSALADAIRVLAMDAVQQANSGHLGAPMGMAEIAQALWT
                     KHLRHNPADPLWANRDRFVLSNGHGSMLIYALLHLTGYDLPLEALQRFRQLHAKTPGH
                     PEVGITPGVETTTGPLGQGLANAVGMALAESLLAAEFNRPGHNIVDHHTYVFAGDGCL
                     MEGISHEACSLAGTLKLSKLVVLYDDNGISIDGHVENWFADDTGKRFEGYGWNVIRGV
                     DGHNVAAVDAAIAAARAQHAKPTLIICRTIIGKGSPNKAGTHDAHGVLDAKEIAATRA
                     ALGWTGGPFQVPQAIYNAWDARDAGLRAQTDWQMAFDTYAIAYPTEAAEFLRRMKGAL
                     PPGFSERMQAFIAQADGKAETVATRKASQLAIAELANLLPELLGGSADLTGSNLTDWK
                     GAQPVRAAEPGSPAGGVAFGRHINYGVREFGMAAIMNGIALHGGHLPFGGTFLTFSDY
                     SRNAIRMAALMKQRVVHVFTHDSIGLGEDGPTHQSIEHAASLRLIPNLSVWRPCDTVE
                     TAVAWTAAVERPASLGMAVRDGGPTALLLSRQNLPFVPRDARTRAAIARGGYVLRDAE
                     GARAILIATGSEVSIALDAQERLKQEGLAVRVVSMPSTDVFDRQSAEWKRSVLPAGLP
                     RVAVEAGVTGGWYKYVGIDGAVVGIDRYGESAPAGTLFKFFGVTAEKVAETVKQLL"
BASE COUNT         1727 a         3686 c         3641 g         1635 t
ORIGIN      
        1 gacgatgacc ccggtcatcg ccgcgacggc cctggccccg gccacgaccg ccaacgacgc
       61 cgccgcgccg cgcaagcgcg ctgccgcgcg caagacggcg ccgcgcgcac cacgcaagac
      121 cgcgacgcgc gccgccgccc cctcccgccg caaaaagtcc gcatgaacgc ccccgccgac
      181 cgcatgcact acgagcggct gatcgcccag gcgcagacgc tgccggccac cgcctgcgcc
      241 gtcgcccacc cctgcgacga gacctcgctg ggcgtggccg tcgaggcggc ccggctcggc
      301 ctgatccagc ccatcctggt gggcccgcag gccaagatcc gcggcacggc cgagcagttc
      361 gggctgagcc tggacggcct ggaaatcgtc gacgcgccgc acagccacgc ggccgccgcc
      421 gcggccgtgg ccctggtgcg caacggccgc gccagcctgc tcatgaaggg cagcctgcac
      481 accgacgagc tcatgcacga agtcgtggcc cgcgacacgg ggctgcgcac cgggcggcgc
      541 atcagccatg tgttcatcat ggacgtgccc acctaccccg agccgctttt catcaccgac
      601 gcggccgtga acatctttcc cgacctggcc accaaggccg acatcgtgcg caacgtgatc
      661 gacctgcaca tcggcctggg gctgggcgag ccgcgcgtgg ccatcctctc ggcggtggag
      721 caggtcaacc ccgacatccc cggcaccatc gacgccgccg cgctgtgcaa gatggccgac
      781 cgcggccaga tcacgggcgg cgtgctcgac ggcccgctgg cgctggacaa cgccatcaac
      841 gaagaagccg cgcgcatcaa gggcatcaag tcgcccgtgg ccggccgcgc ccaggtgctg
      901 gtggttcccg acctggaagc cggcaatatg ctggccaaga acctcacctt cctgtccaac
      961 gcggaagcgg ccggcatcgt gctgggcgcg cgcgtgccca tcatcctcac cagccgggcc
     1021 gacagcccgc gcacgcgcct ggcgtcctgc gccgtggcgg ccatctacgc cgaccatctg
     1081 gcacgcacgc ggcccgtggc ctcctagccg ggcgctccgg cccgcggcac cctcgtccga
     1141 tccttttgta cagcaggcac catgcgcgac accatcctgg tcatcaacgc gggcagttcc
     1201 agcatcaagt tctatctcta cgacatcacc ggccccggac aactgcaggc gcgcctgggc
     1261 gggcaattgt cgggcatcgg cacgccgcac ccgcagttgt cggtgagcag cgccgaaggg
     1321 gcggtcgagg tcgaacgcca gatcgcgccg gcccatgcgg ccgacgtgag caatgcgcag
     1381 gacgtggtcg gcacctggct gagcggccat ctcaacggcg tgccccgggc cgtcgggcac
     1441 cgcgtggtgc acggcggcgc ggagttcacc gggccggtgc tggtcgacga cgccgtgctg
     1501 gccaagctcg atacctatac ggacctggcc ccgctgcacc agccccacaa cctgggcccc
     1561 atccgcgtga tccgcgaacg ccggcccgac ctgccgcagg tggcctgttt cgacacggcc
     1621 ttccaccgca gccacggcga cctggccgac cgtttcgcca tacccgaatc cctgtacgcc
     1681 cggggcgtgc gccgctacgg gttccatggc ctgtcctacg aatacatcgc gcagcggctc
     1741 aaggccgtgc tgcccgagct ggccgccggc aaggtcgtgg ccgcccacct gggctcgggc
     1801 gtgtcggcct gcgccatgca ggccgggcac agcgtcgaca gcaccatggg cttcaccgcc
     1861 atggacggcc tgcccatgag cacccggccc ggccgcctgg acgccggcgt ggtgctgtgg
     1921 atgatggaac agggcatgga ccacgacgcc atcgaggccc tgctctacca cgactgcggc
     1981 ctcaagggcc tgtctggcat cagtggcgac atgcgcgacc tgcaggccag cggcgacccg
     2041 cgcgccaggc tggcgctgga ctacttcgcc tggcgctcgg ccgagggcat cctgggcctg
     2101 ggctgcgcca tgaacggcat tgacggcatc gtcttcaccg ccggcatcgg cgagcattcg
     2161 gccgagatgc gcgcggccat cgccgcgcac tggcaatggc tgggcgtgaa actcgacccc
     2221 gagcgcaacg cccgccacga gcagcgcatc tccgcggacg acagccgcat cggcctgtac
     2281 gtggtgccca ccaacgaaga gctcatcatc gcccagcaca cgctggacct cgtcagccga
     2341 gcctgaatat gacgccgacg cccgcgccgc cccccgccct gcccctggcc ggcaggcgcg
     2401 gcctggtcac cggcatcgcc aacgagaact ccatcgcctg gggctgcgcc cgggccttcc
     2461 gcgcgctggg cgccgaggtg gcggtcacct atctcaacga caagacgcac gcctatgtcg
     2521 agcccctggc gcggcaaatc gaagcgccgc tgctgatgcc cctggacctg ctgcaaagcg
     2581 gccagctgga agccgtgttc gaccagttgc gctcgcattg gggatcgctg gatttcgtgc
     2641 tgcattcgct ggcctatgcg ccgcgcgaag acctgcacgg ccgcgtcgcc gactgctcgc
     2701 gcgacggttt cctgcaggcc atggacgtgt cgtgctggtc gttcatccgc atggccaagc
     2761 tggccgaacc catgatgcaa catggcggcg cccttttctg catgagctac tacggcgcgg
     2821 agaaagtcgt cgagcactac aacatgatgg ggccggtgaa ggccgcgctg gaggcggcca
     2881 cccgctacct ggcggccgaa ctcggcccgc agggcatacg cgtgcacgcg atttcgcccg
     2941 gaccgctcaa gactcgcgcg gcatcgggcc tagaccactt cgacgacctg ctcgaccagg
     3001 cgcaggccaa ggcgcccacg cgcagcctgg tctcgatcga ggacgtgggt gaagccaccg
     3061 cctggctggc caccgatgcg gcgcgccgca tcaccggcca gaccctctac atcgacggcg
     3121 gctatcacat catcgactga gcccttcaga acaggccgcg gaacagccag tacaggccgg
     3181 ccgcgagcgc gatcgagacg ggcagcgtca gcacccaggc catgaccagg ctgcgcaccg
     3241 tgccccactg caggcccgag ccgttggcgg ccatggtgcc cgccacgccg gacgacagca
     3301 cgtgcgtggt ggacaccggc aggccgtaga catcggcggc gccgatggtg agcatggcca
     3361 ccagttcggc cgaggcgccc tggccgtagg tcaggtgctg cttgccgatc ttctcgccca
     3421 ccgtgacgac gatgcgcttc cagcccacca tcgtgcccag gcccagggcg atggccacgg
     3481 cgaccttcac ccaggtgggg atgaacttgg tggcgtggtc gagcgacttg cggtagttgt
     3541 cgacgacgcc ctggtcctgc gcggcgatgg ggttgtcggc gctcttgccg accaggcgga
     3601 tgccttccga caccaggtac atgtcattgc ggatgttctt ggcctggccc tggccgacct
     3661 gcgccatcga acccgaggcc tgcacgtcgc cggcgatgcc gtcggtcagc tgctgcaagg
     3721 ccgacagggt ggccggcgcc agctggcggg tctggacgaa ggactcgacg tcctcgcgcg
     3781 gctgcgccga gatgacgccc ttctcgtact tggcgaaggt ggccgatgcc tgctgggcca
     3841 cggccacgaa ggtgtgcgtc tcggcgggcg tgacggcctt gttcagcgca taggccgtgg
     3901 gcaccgtgcc gatcaggatc agcatgatca ggcccatgcc cttttgcccg tcgttcgagc
     3961 cgtgggcgaa cgacacgccg gtgcaggtca gcaccagcag gccgcggatc cagaacggcg
     4021 gcggggcgtt gccggcgggc tccttgtaga gcgcgggcac cttcaccagc gccttgagcg
     4081 ccagcagcag gaccgccgac agcacgaagc cgaccaccgg cgagaacagc agcgccttgc
     4141 ccacgcccag cgcctggtcc cagtccacgc cgctggtgcc ggaggggccg tgcagcatct
     4201 ggttcatcag gcccacgccg atgatcgagc cgatcaaggt gtgcgaactc gacgacggca
     4261 ggccgaaata ccaggtgccg aggttccaga tgatggccgc gatcaggagc gcgaacacca
     4321 tggcgaagcc cgcgctgctg cccacttgca ggatgagctc gaccggcagc agctgcagga
     4381 tgccgaaggc caccaccccc gtcgaggtca gcacgcccag gaagttccac aaaccggacc
     4441 agaccacggc caggttgggc gccatggagc gcgtgtagat caccgtggcg accgcgttgg
     4501 ccgtgtcgtg aaagccgttg acgaactcga agcccagcgc gatcagcagc gccacgccca
     4561 acaggatgta gggcagggac gaaggcatgg agaccggctc caggtcggcg gcgatatgcg
     4621 tcacgacata gccgaccccg acgatcagga acaggaagaa gacggccagg cccagatacc
     4681 gggaacgggc ggggggcgcc gcgacgggcg aaaccagagt ttgcgacatg gtcagaccaa
     4741 ggaagttgaa ccgaccatga tgctattgac ggcatttgtc acttccatga cgccgctttg
     4801 ggacaatggt aagcaaatct ggcagaaacc ttgtcgcgct actattgccc gtagatccgc
     4861 cccccgtcag gccctgcccc ttttttgccg ccgggccgcc ccaaggcgaa aacggccccc
     4921 tcggagggca gcaagccgac ggcgcagcgt ggggcctttt ttgcacctgc catgaccctt
     4981 gccctgacgc ctgcgacctt cgacgccgac atttccggcc tgctctatgc cttccgcttc
     5041 cagccgggcc agcccggcca ggagctcgac agcgccagcg cgcaagacgc gctgggccag
     5101 ctcgacgaga gccacggcgg cccgacccag gacttcctct ggctgcattt caacctgtcg
     5161 caggccggct gcacgcactg gctgcagcag cacctgaaca tacccgacga attcttcgag
     5221 atgctgggcg agcaggtgca ttccacccgg ctcgaacggc aggacgacgc cctggtggcc
     5281 gtgttcaacg acgtgatctt cgagctcgac cacacgccgt cggacgtcac cacgtcgtgg
     5341 gtctacgcgc accgcaacct gatgatctcg atgcgctaca agcccctgcg ctccatcgac
     5401 cggctgcgca tgtcgatccg gcgcggcgag accttcgagt ccaccgccga cctgctgtcg
     5461 cacctgatgc gcgagcaggc cgacctcatg gtggagatcg tccgccgcgc caacaccgag
     5521 gtcgaccgcg tcgaagaccg cttcctggcg gccgacacgg ccggcaaccg gcaaacgcta
     5581 tcgtccaacc ggcgcgtgct ggtgcgcctg cagcgcatgc tggcgcccga gcccggctcg
     5641 gtcttccgcc tgctggcccg cccgccctac tggctgaccc aggacgacgt gcagagcctg
     5701 cgcgaatcca ccgaggaatt ctcggtcgtg ctgggcgaca tgaccggcct gatcgaacgc
     5761 atcaagctgc tgcaggaaga aatcgccgcc aacctgcagg aacagaacaa ccgcacgctc
     5821 ttcgtcctga cgctggtcac cgtgctcgcc ctgccgatca acatggtcgc cggcctgttc
     5881 ggcatgaacg tgggcggcat accgctggcc agcgatccga acggcttctg gttcctggtg
     5941 ctgtgcgtga tcgcgttcac cgggctgctc gcctggcggt cgctgcggcg cgcgaaccgg
     6001 tgagaatcag gccgcgcgcg cttgcaggat agccacggcc ggcagcgcct tgccttccag
     6061 gaactccagg aaagcgccgc cgccggtgga gatatagtcc acctgctcgc cgatgccgta
     6121 cttggcgatg gcggccaggg tatcgccgcc gccggcgatg gagaaacccg ccgcgcaagc
     6181 gatggccttg gacacggtct cggtgcctcc ggcgaactgg tcgaactcga acacgcccac
     6241 cggtccgttc cagacgatgg tgccggcgcg cgcgatgatc tcggccaggc cctgcgcggt
     6301 cttgggaccg agatcgaaga tcatgtcgtc ggcctcgacc tcgccggccg ccttggtgac
     6361 ggccgcggca tcggggctga actgcttgcc gcacaccacg tccaccggga tgggcaccgc
     6421 ggcgccgcgc tgctgcatca tctcgatgac ggcgcgggcc tgctcgacct ggtcgggctc
     6481 ggccagcgac ttgccgatgg gcaggccgca ggccaacaag aaggtattgg cgatgccgcc
     6541 gcccaccacc agctggtcga ccttgccggc cagcgactgc aggatggaca gcttggtcga
     6601 caccttggag cagccgacga tggccaccag cgggcgcttg ggttcgtgca gggcgcggcc
     6661 cagggcatcg agctcggccg ccagcaaggg gccggcgcag gcgatgggcg cgaagcgcgc
     6721 catgccgtgc gtggtggcct cggcgcggtg cgccgtgccg aaggcgtcgt tcacgtagac
     6781 atcgcacagg gcggccatct tgcgcgagag cgtctcgtcg tccttctttt cgccctggtt
     6841 gacgcggcag ttctccagca gcaccacctc gcccggcgca acggccacgc cgtcgaccca
     6901 gttctgcacc agcttcaccg gcttgcccag cagctcggcc aggcgccggc ccacgggggc
     6961 cagcgtatcg ccggggccca gcgtgccctc ggtgggccgg cccaggtgcg aggtcaccat
     7021 cacggccgcg ccggcctcca gcgccagcgc gatgccgggc accgaggcgc ggatgcgggt
     7081 gtcttcggaa atattgccgg cgtcgtcgaa gggaacgttc agatcggcgc ggatgaaaac
     7141 gcgcttgccg gacaaagcgc cggacttggc cagcgcagta agggtttgga ctttggacat
     7201 tgtcgtcatt cgaatgtcgc catcatgcga tgacggagcc gaggaccccg gaggaatccg
     7261 aagcgcagcc ccttttcagg ggcgagcatc ggaatgccgc agggcagcct cggcggatca
     7321 agaaaaatgg ccggggcatg aagcatgccg gccatcccat gtgtgtccga taaccgagcc
     7381 gagacttact tggccgccat catcgcaccg gccgtatcga gcatgcggtt cgagaaaccc
     7441 cactcgttgt cgtaccaaga agtcaccttc accagcctgc ccgacacctt ggtcagcgtg
     7501 gcatcgaagg tgctggaagc cgggttgtgg ttgaagtcgc tcgagaccag gggcgcggta
     7561 ttgtaggcca ggatgccctt gagctcgcct tcggaagccg ccttgaggat ggcgttgact
     7621 tcctcgaccg tggtgtcgcg cttggcgatg aacgacaggt cgacgatgga cacattgatg
     7681 gtcggcacgc ggatggcgaa accgtcgagc ttgccgttga gctcgggcag caccaggccc
     7741 accgcggcgg cggcgccggt cttggtgggg atcatgttct gcgtggccga gcgggcgcgg
     7801 cgcaggtcct cgtggtagac gtcggtcagc acctggtcgt tggtgtagga gtgcaccgtg
     7861 gtcatcaggc cggactccag gcccagcttg tcgttcagcg gcttgaccag cggggccagg
     7921 cagttggtgg tgcaggaggc gttggacacc acggtcatcg ccgaggtcag cacgttctgg
     7981 ttgacgccgt aaacgatggt ggcgtcgacg tccttgccgc cgggagccga gatcagcacc
     8041 ttcttggcgc cacccctgag gtgggcgctg gccttttcct tggaggtgaa gaagcccgtg
     8101 cactccatca ccacgtccac gcccagctcg ccccagggca gctcggcggg gttgcggttg
     8161 gccagcacgc ggatcttgtc gccattgacg accatgtagt cgccgtcgac cgagacctcg
     8221 ccgggaaagc ggccgtgcgc ggtgtcgtac tgcgtcaggt gggcattggt cttgggatca
     8281 cccaggtcgt tgatggcgac gatctcgagg tcgtgcttct tgccggactc gtagtgggca
     8341 cgcagcacgt tgcggccgat gcggccgtag ccgttgatgg cgacgcgaat agtcatgaca
     8401 ggctcctttg ggactttgat tacgtgatgg attacaaaag ctgcttgacg gtctcggcca
     8461 ccttctcagc ggtcaccccg aagaatttga acagcgtgcc ggcgggcgcg gattcgccgt
     8521 agcggtcgat gccgaccacg gcgccgtcga tgccgacata cttgtaccaa ccgcccgtga
     8581 cgccggcctc gacggccacg cgcggcaggc cggcgggcag aaccgagcgc ttccattcgg
     8641 cgctctgcct gtcgaacacg tcggtgctgg gcatggacac cacgcgcacg gccaggcctt
     8701 cctgcttgag gcgctcctgc gcgtccagcg cgatggacac ctcggagccg gtggcgatga
     8761 ggatggcgcg ggcgccttcg gcgtcgcgca gcacgtagcc gccgcgcgcg atggccgcgc
     8821 gcgtcctggc gtcgcgcggc acgaaaggca ggttctggcg cgacagcagc agcgccgtgg
     8881 gaccgccgtc gcgcacagcc atgccgaggc tggccggacg ctcgaccgcg gccgtccagg
     8941 ccacggcggt ctcgaccgtg tcgcacggac gccagaccga caggttgggg atcaggcgca
     9001 ggctggccgc atgctcgatg gactggtggg tggggccgtc ctcgcccagg ccgatggagt
     9061 cgtgggtgaa cacgtgcacc acgcgctgct tcatcagcgc cgccatgcgg atggcgttgc
     9121 gcgagtaatc ggagaaagtc aggaaggtgc cgccgaaggg caggtggccg ccgtgcagcg
     9181 cgatgccgtt catgatggcg gccatgccga actcgcgcac gccgtagttg atgtggcgcc
     9241 cgaaggccac gccgccggcg gggctgccgg gctcggcggc gcgcacgggc tgcgcgccct
     9301 tccagtcggt gaggttggag ccggtgaggt cggccgagcc gcccagcagt tcgggcagca
     9361 ggttggccag ctcggcgatg gccagttgcg aggccttgcg ggtggccacg gtctcggcct
     9421 tgccgtcggc ctgcgcgatg aaggcctgca tgcgctcgct gaaacccgga ggcagcgcgc
     9481 ccttcatgcg gcgcaggaac tcggccgcct cggtggggta ggcgatggcg taggtgtcga
     9541 aggccatttg ccagtcggtc tgcgcgcgca ggccggcgtc gcgggcgtcc caggcgttgt
     9601 agatggcctg gggcacctgg aaggggccgc ccgtccagcc cagcgcggcg cgcgtggcgg
     9661 cgatttcctt ggcgtccagc acgccgtgcg cgtcatgcgt gccggccttg ttgggcgacc
     9721 ccttgccgat gatggtgcgg cagatgatga gggtgggctt ggcgtgttgc gcgcgggccg
     9781 cggcgatggc cgcgtcgaca gccgccacgt tgtggccgtc cacgccgcgg atcacgttcc
     9841 agccgtagcc ctcgaagcgc ttgccggtgt cgtcggcgaa ccagttctcg acgtggccgt
     9901 cgatggagat gccgttgtcg tcgtacagca ccacgagctt ggacagcttg agcgtgccgg
     9961 ccagcgagca ggcctcgtgc gagatgcctt ccatcaggca gccgtcgccg gcgaacacat
    10021 aggtgtggtg gtcgacgatg ttgtggccgg ggcgattgaa ctcggccgcc aggagcgatt
    10081 cggccagcgc catgcccacg gcattggcca ggccctggcc cagcggaccg gtggtggttt
    10141 ccacgccggg ggtgatgccg acctcggggt ggcccggggt cttggcatgc agctggcgaa
    10201 agcgctgcag cgcttcgagc ggcaggtcgt agccggtgag atgcaagagc gcgtagatca
    10261 gcatcgagcc atggccgttg gacagcacga agcggtcgcg gttggcccac agcggatcgg
    10321 ccgggttgtg gcgcaggtgc ttcgtccaga gcgcctgggc gatctcggcc atgcccatgg
    10381 gcgcgcccag atgcccggaa ttcgcttgtt gtacggcgtc cattgcgagg acgcggatgg
    10441 catcggccag ggcggaaggg gtaggcaggc tcatggagtg gcggggatct tggaaatgaa
    10501 ggccggaacg cgaagacggc agcgcctcgg aaaaaggcgt gcgaaagccc aagattttac
    10561 cacccgatgc cgcgccggct ttggcgccat ggctatcctg ttatcgttgc gaaccgattg
    10621 tttcaaccct gttttggccc ggccgccatg ccccgcttct actgccccgc cccgcttgcc
    10681 gcgggcacg
//
LOCUS       DASWPO010000300         9601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_875978, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000300 DASWPO010000000
VERSION     DASWPO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875978"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VGC69_15830"
     CDS             complement(<1..1218)
                     /locus_tag="VGC69_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0569 family flavin-dependent
                     oxidoreductase"
                     /protein_id="HEY0296797.1"
                     /translation="MANTATPADAPADRHYSVIIVGGGQAGLSLSVYLQQRQIDHLVI
                     EKHSLAHTWRTQRWDAFSLVTPNWQCNLPGWPYQGDDPHGFMVKDQINAWLAGFVAQT
                     QAPALEGTAVQRVWRDDPQSAYQVQTSQGRFTADQVVVASGGYHVPMVPRCAERLPDR
                     VRQVHSADYRNPAQLPEGAVLVVGSGQSGAQIAEDLHLAGRKVYLAVGNAPRCARFYR
                     GKDVVDWLADMGYYDMPVTAHPLREGVRDNTNHYVTGRDGGRDIDLRKFASEGMELYG
                     VLRDFDGADLTFDANVKSALDEADKTYNGINAAIDKFIAENAITAPPPSVYSPVWEPP
                     AERPTLDLASSGITSIVWCIGFRPDFRWLDAPVFNGDGHPKHHRGITTQEGVYFIGLP
                     WLHTWGSGRFGSVG"
     gene            complement(1261..1542)
                     /locus_tag="VGC69_15835"
     CDS             complement(1261..1542)
                     /locus_tag="VGC69_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0570 family nitrogen starvation response
                     protein"
                     /protein_id="HEY0296798.1"
                     /translation="MPAMYFHVRWPDQQETRCYSPSLVIQDYLRPGGVYALDEFLAKV
                     REALNIASERVRAKYGFACSQALDQLAEIEQIAARFDGGSPITVTGFRL"
     gene            complement(1560..2747)
                     /locus_tag="VGC69_15840"
     CDS             complement(1560..2747)
                     /locus_tag="VGC69_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0565 family glycosyltransferase"
                     /protein_id="HEY0296799.1"
                     /translation="MPDSASLAIGLLTHSVNPRGGVVHTLELAEALRLRGHRVTVIAT
                     ARPGQRFFRPVASEVALIDLPGEAHPDMVQEIGTRIARTEQYLRQHLAQRSFDILHAQ
                     DPIGGNALANLRDQARIAHFVRTVHHLDHFSEPQLDAWQTRGYQAADLVLCVSAMWQE
                     RLLQEHGQASGLVHNGVDTRRYTPQAHPDDEALRRRLGIRADGRVVLSIGGIEERKNT
                     VRLLQAFIPLRDRMPDAQLVIAGGASLLDHSGSARAFHALLARHGMAHGPGQPVLITG
                     PLPDDDIPGLLRLADVTAMPSLREGFGLVVLESLACGTPVVVSRIRPFIDYLAPGDAC
                     WADPMSPSSIEQALEGALAETRPAQVEATARRLSASYAWDASARQHLAHYGRLLQAEL
                     SAT"
     gene            complement(2740..3750)
                     /locus_tag="VGC69_15845"
     CDS             complement(2740..3750)
                     /locus_tag="VGC69_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HEY0296800.1"
                     /translation="MSALSELIAGLRASRGFAHKTDISDVVGALARATPPDAPAAALS
                     VPIGDDCAAMPDGQGGHHLLAIEGLVEDFIVRMPWFAGYSAVMVNISDIYAMGGRPTA
                     VVNALWSTGMNPAREMIEGMASACARYGVPMVGGHSNNRSDRPQLAVAIAGHAKKLLT
                     SFNARPGDSLLMAVDLRGQWQDPFPYWNASTQAPAQRLRDDLDLLPRLAESGRCDAAK
                     DISMAGVLGTALMLLECSGVGARVDLDAIPHPGDVPMLRWLTAFPSYGFILSVRPGQA
                     QDVIGAFAGRGLACAAIGEVTAGRRLVLRQGDEQGLLWDIDAQPFIRAAQPRPAGEMI
                     HA"
     gene            complement(3747..4322)
                     /locus_tag="VGC69_15850"
     CDS             complement(3747..4322)
                     /locus_tag="VGC69_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HEY0296801.1"
                     /translation="MHSCLLDASPLRAEPSAPVLPCAYLVRRAIHACEFDEAYALRRA
                     VFCVEQGIFLRDDRDAIDDRAQLLVAQACEAGMPTQVVGTVRIHEAEPGVWWGSRLAV
                     HASFRTLGHLGATLIRLAVSSAHAQGCERFMAQVQPQNAALFERLHWQPLREVLAHGR
                     PHVEMQASLAHYPPCHDPVSGFVSRRSRGAS"
     gene            complement(4337..5407)
                     /locus_tag="VGC69_15855"
     CDS             complement(4337..5407)
                     /locus_tag="VGC69_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HEY0296802.1"
                     /translation="MTELQSYGLRLVDPSAGAASRRGGAGPSDHKAVTVDGHTIMVPV
                     HTASAWDSPYVAQAPDAQGVSVITREAIPIARISFPRQPRFYKLQTLDGVPYSHIATL
                     HGADVLATTVLQTCIRYESRRKMCQFCAIGQSLAAGRTVARKTPEQLAEVARMAVLLD
                     GVKHMVMTTGTPNATDRGAAILCESAFAVKAAVDLPIQGQCEPPDDDRWFERMRASGI
                     DTLGMHLEAVSPEVRARIMPGKAAVSIERYLGAFEAAVDVFGRGQVSTYILAGLGDTR
                     EAVLAMCSQVLARGVYPFVVPFVPISGTPMEGHPAPSPAFMQSILEPLGRMVAEAGLR
                     SADIKAGCGKCGACSSLSVYEN"
     gene            complement(5370..6434)
                     /locus_tag="VGC69_15860"
     CDS             complement(5370..6434)
                     /locus_tag="VGC69_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000257 - nitrilase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nit6803 family nitrilase"
                     /protein_id="HEY0296803.1"
                     /translation="MASPRMVRAAAVQIAPDLDRPDGTLDRVCAAIDEAAAQGAQIAV
                     FPETFVPYYPYFSFVLPPCQQGAPHLELYERAVVVPGPVTQAVAERARARSMVVVLGV
                     NERDHGSLYNTQLLFDADGSLKLKRRKITPTYHERMVWGQGDAAGLKVVDTAVGRVGA
                     LACWEHYNPLARYALMAQHEEIHCAQFPGSLVGQIFADQMWVAIRHHALESGCFVINA
                     TGWLTDAQIQSITPDAGLQRALRGGCHSAIVSPEGKYLAEPLTEGEGMVIADLDLSLV
                     TKRKRMMDSVGHYARPELLSLAINDRPAQPMFAMPAGAETRAAPAPSSSTGASHDHQS
                     SHPGQPAADDRAAVLRAAAG"
     gene            complement(6469..6951)
                     /locus_tag="VGC69_15865"
     CDS             complement(6469..6951)
                     /locus_tag="VGC69_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HEY0296804.1"
                     /translation="MPKVTRPANQKGEFLVDYEEKVFEDVKAEPGEKALVTFHTVAFE
                     GSIGFVNLLQATRLQRKGFETSILLYGPGVTLGVKRGFPTLGDSAFPGHQNFNDQIAK
                     FITEGGKVYACRFALQALYGHGEGALIEGIRPINPLDVLDLVLLHRKANAFILDTWTL
                     "
     gene            7368..8762
                     /locus_tag="VGC69_15870"
     CDS             7368..8762
                     /locus_tag="VGC69_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0296805.1"
                     /translation="MAATPQHWSRRIREGGKPAYILIAEIIAEDIHKGLLSVRDPLPT
                     LRDLARDLGLNYTTVARGYAEARKRGLIESHVGIGTVIRGSHPGLPIQSGTNAAMTMN
                     SPPEPTQPELLAQLEHEAATLFEPNLGSGLYDLLRYQDFGGTHQDREAGVQWLRSHLP
                     RCDASQVLVAPGIHSVLMATISMLAKPGQTICVESLCYPGIKAMAAQFNVKLQALPMD
                     DQGVDPDALEHVCKTQRPKALICNPTIHNPTTATMGKERREAIADIALRYSIPIVEDD
                     AYAKLGDEDLPTLSSLAPELTYYVGGLSKSLGAGLRIAYLHAPTDLQTQRLAGALRAT
                     TVMASPVTAALATRWIVSGLAYRMLDEVRNESIARQALAARILDDFDVQAHPDGFHLW
                     IKVDGNPVELALSLRARGVPCVASVAFSTDAAPPAAIRLCLGGNLSRAEAQQSLHLVA
                     TALRQAAGPDEHAL"
     gene            complement(8787..>9601)
                     /locus_tag="VGC69_15875"
     CDS             complement(8787..>9601)
                     /locus_tag="VGC69_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0296806.1"
                     /translation="VRFDAHTGAVLEQSKPPGQQRQTFLGLMLHLHRDLFADLPGELF
                     LGVMALLFVLAIVSGIVLYGPFTRKLDFGTVRAGRSARIRWLDRHNLLGVTTLAWTLG
                     VGVTGLMNELSTPLFALWRQTDVQAMLAPWHGKPAPSQADWTPVDAAFGTARLALPGM
                     VVTSVTFPGSPFGSPYHYLLWAKGDTPLTSRLFSPVLVDARTGELAAVVKMPWYLRAL
                     EVSRPLHFGDYGGMPLKILWALLDLITIVVLASGLYLWLARRESSAAARPAK"
BASE COUNT         1604 a         3210 c         3339 g         1448 t
ORIGIN      
        1 tccgacgctg ccgaagcgcc cggaacccca tgtgtgcagc caaggtaggc caatgaagta
       61 aacgccttct tgcgtggtga tgccgcgatg gtgcttgggg tgcccgtcgc cattgaacac
      121 cggtgcatcg agccagcgga aatccggcct gaaaccgatg caccagacga tggaggtgat
      181 accggaactt gccagatcga gggtcgggcg ctcggcaggg ggctcccaga cgggcgagta
      241 gacggacggt ggcggggcgg tgatcgcgtt ctcagcgatg aacttgtcga tggcggcgtt
      301 gatgccgtta taggtcttgt cggcctcgtc cagcgcggat ttcacattgg cgtcaaaggt
      361 cagatcggcg ccgtcgaagt cgcgcagtac gccgtacaac tccatgccct cgctggcgaa
      421 tttgcgcagg tcgatgtcgc gcccgccatc gcggccggtg acgtagtgat tggtgttgtc
      481 gcgcacgcct tcacgcagcg ggtgggcggt caccggcatg tcgtagtagc ccatgtcggc
      541 cagccagtcc accacgtcct tgccgcggta gaaccgggcg cagcgcggcg cattgccgac
      601 ggccaggtac accttgcggc cggccaggtg caggtcttcg gcgatctgcg cgccggactg
      661 cccgctgccc accaccagca cggcgccttc cggcaactgg gccgggttgc ggtagtcggc
      721 cgaatgcacc tgcctcacgc ggtcgggcag gcgctcggcg cagcggggaa ccatgggcac
      781 gtggtagccg ccggaggcga ccaccacctg gtcggcagtg aatcggccct ggcttgtctg
      841 gacctggtag gcagactgcg gatcgtcgcg ccagacccgc tggactgccg tgccctcgag
      901 cgccggcgcc tgggtttgcg cgacgaagcc ggccagccac gcgttgatct ggtccttcac
      961 catgaagccg tgcggatcgt cgccctggta aggccagccg ggcaggttgc actgccagtt
     1021 gggcgtgacc aggctgaagg catcccagcg ctgcgtacgc caggtgtggg ccaggctgtg
     1081 cttttcgatg acgagatgat cgatctggcg ctgctgcagg tagacgctca gcgacaggcc
     1141 agcctggccg ccgccgacga tgatgacgct gtagtggcga tcggcaggcg catcggcagg
     1201 cgttgcggtg tttgccatgt tgttctcctt gatggcggat cggaagcgga agggacgggc
     1261 ctagagtctg aaacccgtca ccgtaatggg cgagccgccg tcgaaacggg cggcgatctg
     1321 ttcgatttcg gcgagctggt ccagggcctg ggaacaggcg aagccgtact tggcgcgcac
     1381 gcgctcggag gcgatgttca aggcctcgcg caccttggcc aggaactcgt ccaaggcata
     1441 aacgccgccc ggccgcaggt agtcctggat gacgagcgag ggcgaatagc agcgggtttc
     1501 ctgctggtcg ggccatcgca cgtggaaata catcgcgggc atgggggctc cgtggaaaat
     1561 caggttgcag aaagttcggc ctgcagcagc cggccgtaat gcgccaggtg ctggcgggca
     1621 ctggcgtccc aggcgtaact ggcggacagc cgccgggccg tggcctcgac ctgggccggg
     1681 cgggtctcgg ccaaggcccc ttccagcgcc tgctcgatgg aggacggcga catcgggtcg
     1741 gcccagcagg catcgcccgg ggcgaggtaa tcgatgaacg gccggatgcg ggagaccacc
     1801 accggcgtgc cgcaggccag ggactccagc accaccaggc cgaagccttc gcgcagggag
     1861 ggcatggcgg tcacgtcggc aagccgcagc aggccgggta tgtcgtcgtc gggcagcggg
     1921 ccggtgatca gcaccggctg ccccgggcca tgcgccatgc cgtgccgtgc cagcagcgca
     1981 tggaaagccc gggcgctgcc gctatggtcc agcaggctcg cgccgccggc gatgaccaac
     2041 tgcgcgtcgg gcatccggtc gcgcagcggg atgaaggcct gcagcagccg cacggtgttc
     2101 ttgcgctctt cgatgccgcc gatgctcagc acgacccggc cgtccgcgcg gatgcccagc
     2161 cggcggcgca gcgcctcgtc gtccgggtgc gcctgcggcg tgtagcggcg ggtatcgacg
     2221 ccgttgtgca ccagcccgct cgcctgcccg tgctcttgca gcagccgctc ctgccacatc
     2281 gcgctgacgc acagcacgag atccgccgcc tggtagccgc gcgtctgcca ggcgtcgagc
     2341 tggggctcgc tgaagtgatc caggtggtgg acggtacgga caaagtgcgc gatgcgggcc
     2401 tggtcgcgca ggttggccag ggcattgccg ccgatgggat cctgcgcgtg caggatgtcg
     2461 aacgatcgct gcgccaggtg ttgccgcagg tactgctcgg tgcgggcgat gcgcgtgccg
     2521 atttcctgga ccatgtcggg gtgcgcttcg ccgggcaagt cgatcagcgc gacctcgctg
     2581 gcgacggggc ggaaaaagcg ctgcccgggc cgggccgtcg cgatcacggt gacgcgatgg
     2641 ccccgcaggc gcagcgcctc ggcgagctcc agcgtatgca ccacgccgcc gcgcgggttg
     2701 acggagtgcg tcagcagccc gatggcaagg gatgcggagt caggcatgga tcatctcccc
     2761 ggccggccga ggctgcgccg cgcggatgaa gggttgcgcg tcgatgtccc agagcaggcc
     2821 ctgctcatcg ccctggcgca ggacaagccg gcggccggcc gtcacctcgc cgatggcggc
     2881 gcaagccagg ccgcggccgg caaacgcccc gatcacatcc tgcgcctgcc cgggacgcac
     2941 gctcaggatg aagccgtagc tgggaaaagc cgtgagccag cgcagcatcg gcacgtcgcc
     3001 gggatgcggg atcgcatcga gatcgacgcg ggcgcccacc ccggagcact ccagcagcat
     3061 gagcgccgtg cccaggacgc ccgccatgct gatgtccttg gcggcatcgc accggccgga
     3121 ctcggccagg cggggcagca ggtccaggtc gtcccgcaag cgctgcgccg gcgcctgcgt
     3181 cgaggcattc cagtaaggga aaggatcctg ccattgcccg cgcaggtcca cggccatgag
     3241 caggctgtcg ccgggccggg cattgaaact cgtcaacaat tttttggcat ggccggcgat
     3301 ggccacggcc agctgcggcc ggtcgctgcg gttgttgctg tgcccgccca ccatgggcac
     3361 gccatagcgc gcgcaggcgc tggccatgcc ttcgatcatt tcgcgcgccg ggttcatgcc
     3421 cgtgctccac aacgcattga cgaccgccgt cggacggccg cccatggcgt agatgtcgct
     3481 gatattgacc atgacggcgc tgtagccggc gaaccagggc atgcgcacga tgaagtcctc
     3541 caccagcccc tcgatggcca gcaggtgatg gccgccctgc ccgtccggca tggccgcgca
     3601 gtcgtccccg atgggcaccg aaagcgccgc ggccggcgca tccggcggcg tggcccgcgc
     3661 cagagcgccc accacgtcgc tgatgtcggt cttgtgggcg aagccgcggc tggcccgcag
     3721 gcccgcgatc agttcggaca gcgcgctcat gacgcccccc tgctgcggcg ggagacgaaa
     3781 ccgctgaccg ggtcgtggca cggcgggtaa tgggccaggc tggcctgcat ctcgacatgc
     3841 ggccggccgt gcgccagcac ctcgcgcaag ggctgccaat gcaggcgctc gaacagggcc
     3901 gcgttctgcg gctggacctg ggccatgaaa cgctcgcagc cctgggcatg cgcgctcgag
     3961 acggccagcc ggatcaaggt cgcgcccaga tggcccagcg tgcgaaacga cgcatgcacg
     4021 gccaggcgcg atccccacca cacgcccggc tcggcctcgt ggatgcgcac cgtgcccacc
     4081 acctgcgtgg gcatgccggc ttcacaggcc tgcgccacca gcagctgggc gcggtcgtcg
     4141 atggcgtccc ggtcgtcgcg caggaagatg ccctgctcca cgcagaacac cgcgcggcgc
     4201 aaggcatagg cttcgtcgaa ctcgcacgcg tggatcgccc gccgcaccag gtaggcgcac
     4261 ggcaagacgg gagcggacgg ctcggcccgt agcggggaag catcaagcag acaggagtgc
     4321 atgcggcctc cggggctcag ttttcgtaga ccgacagcga cgagcaggcg ccgcacttgc
     4381 cgcagccggc cttgatgtcg gccgaacgca ggcccgcctc ggccaccatg cggcccagcg
     4441 gctccaggat ggactgcatg aaggcgggcg acggcgcggg atgcccttcc atcggcgtgc
     4501 cgctgatcgg cacgaaaggc acgacgaagg gatagacgcc gcgcgccagg acctgggaac
     4561 acatcgccag aacggcctcg cgcgtgtcgc ccagtccggc caggatgtag gtgctgacct
     4621 ggccgcggcc gaacacatcc accgccgcct cgaaggcgcc caggtagcgc tcgatggaca
     4681 cggcggcctt gcccggcatg atgcgcgcgc gcacctcggg gctcacggct tccaggtgca
     4741 tgcccagggt gtcgatgccg gacgcccgca tgcgttcgaa ccagcggtcg tcgtccggcg
     4801 gctcgcattg cccctggatg ggcaggtcga ccgcggcctt cacggcgaag gcgctttcgc
     4861 acaggatggc cgcgccacgg tcggtggcgt tgggcgtgcc cgtggtcatg accatgtgct
     4921 tgaccccatc gagcagcacc gccatgcggg ccacttccgc cagttgttcg ggcgtcttgc
     4981 gggcaacggt gcgccccgcc gccagcgact ggccgatggc gcagaactgg cacatcttgc
     5041 gccggctctc gtagcggatg caggtctgca gcaccgtggt ggccagcacg tcggcgccgt
     5101 gcagcgtggc gatatgcgaa tacggcacgc cgtcgagcgt ttgcagtttg taaaagcgcg
     5161 gctgcctggg aaaggaaatc cgcgcgatgg gaatggcctc gcgcgtgatc acgctcacgc
     5221 cctgcgcgtc cggcgcctgg gcgacgtacg gcgaatccca ggccgaggcg gtgtgcaccg
     5281 gcaccatgat ggtatgcccg tccaccgtca cggccttgtg gtcggacggc cccgcgccgc
     5341 ctcggcggct ggccgccccg gcgctcggat caaccagccg cagcccgtag gactgcagct
     5401 cggtcatcag ctgccggttg gccggggtgg gaggattggt ggtcatggga tgctccagtg
     5461 gatgacgacg gggccggcgc ggcgcgcgtc tcggcgccgg caggcatggc gaacatgggc
     5521 tgggccgggc ggtcgttgat ggccaggctc agcagctcgg ggcgggcata gtggccgacc
     5581 gaatccatca tgcgtttgcg cttggtgacc agcgacaggt ccaggtcggc gatgaccatg
     5641 ccttcgcctt cggtcagcgg ctcggccagg tacttgcctt ccggcgaaac gatggcgcta
     5701 tggcagccgc cgcgcagcgc ccgctgcagg cccgcgtcgg gcgtgatgga ctggatctgc
     5761 gcgtcggtga gccagcccgt ggcgttgatg acgaagcagc ccgactctag cgcatggtgg
     5821 cggatggcca cccacatctg gtcggcgaag atctgcccca ccagcgagcc gggaaactgg
     5881 gcgcagtgga tttcttcgtg ctgcgccatc agggcatagc gggccagggg gttgtagtgc
     5941 tcccagcagg ccagggcgcc gacccggccc acggcggtat cgaccacctt gaggccggcc
     6001 gcgtcgccct gcccccagac catgcgctcg tggtaggtgg gcgtgatctt gcggcgcttg
     6061 agcttgaggc tgccgtccgc atcgaacagc agctgggtgt tgtagaggct gccgtggtcg
     6121 cgctcgttga cgcccagcac cacgaccatg gaccgcgcgc gggcgcgctc ggccacggcc
     6181 tgggtgacgg ggccgggcac gaccacggcg cgttcgtaga gttccaggtg cggcgcgccc
     6241 tgctggcagg gcggcagcac gaaagagaaa tacgggtagt acgggacgaa ggtttccgga
     6301 aagacggcga tctgcgcgcc ttgcgcggcg gcttcgtcga tggcggcgca gacgcggtcg
     6361 agcgtgccgt cggggcggtc gagatccggc gcgatctgca cggcggcggc gcgaaccata
     6421 cggggtgaag ccatggtggc gtattcctct ttcgggacgc tgcgttgctt acagggtcca
     6481 ggtgtccagg atgaaggcgt tggccttgcg atgcaggaga accaggtcga gcacgtcgag
     6541 cgggttgatc ggacggatgc cttcgatcaa ggcgccttcg ccatggccgt agagcgcctg
     6601 cagggcgaag cggcaggcgt agaccttgcc gccctcggtg atgaacttgg cgatctggtc
     6661 gttgaagttc tggtgcccgg gaaaggcgga gtcgcccagc gtcgggaagc cgcgcttgac
     6721 gcccagggtg acgcccgggc cgtagagcag gatcgacgtc tcgaagccct tgcgctgcag
     6781 gcgcgtggcc tgcagcagat tcacgaagcc gatcgaacct tcgaaagcga cggtatggaa
     6841 ggtcacaagc gctttttcgc cgggctcggc tttgacgtct tcgaacacct tctcttcgta
     6901 gtcgacaagg aattcgcctt tctggttggc gggacgggtg actttgggca tggttgttct
     6961 cctgttggag ttgtgggacg cggtcgagcc gcaggcaatc aatcccatgc aacgcgtatg
     7021 ccaaacacaa agcatcccag cacaaaaaaa acgatcaata tgcaatcaag gccaattggc
     7081 gtggcatgcg cgccgatccc atgaaaaaac gcggccccag cacggggaat acccctgcct
     7141 tgcatgcctc gttttggaaa aaatccgtgc aatcaatgca cgcgatcatt gatgttcatg
     7201 caccaagacc agcacgaatg ctcgctacca gtgcacgaaa gcaaggctct tcggaaaacg
     7261 gcgacacact tgatcgacga taaatttgca atcaactcca tgccatagaa caatcaatcg
     7321 acacacagtg cgtgtgatcg aaaaacgcca aacaaggggc attggacatg gccgcgacgc
     7381 cgcaacactg gtccaggcgc atccgggaag gcggcaagcc cgcctacatc ctgatcgccg
     7441 aaatcatcgc cgaagacatc cacaaaggcc tgctgtccgt gcgcgaccca ttgcccacgc
     7501 tgcgcgacct ggcgcgcgac ctggggctga actacaccac ggtggcgcgc ggctatgccg
     7561 aggcgcgcaa gcgcgggctc atcgagtcgc acgtgggcat cggcacggtg atacgcggca
     7621 gccatcccgg cctgcccatc cagtcgggca ccaacgcggc catgacgatg aactcgccgc
     7681 ccgagccgac gcagcccgag ctgctggcgc agctggagca cgaggccgcg accttgttcg
     7741 aacccaatct gggcagcggc ctctacgacc tgctgcgcta ccaggacttc ggcggcacgc
     7801 accaggaccg cgaggccggc gtgcaatggc tgcgctccca cctgccgcgc tgcgatgcct
     7861 cgcaggtgct ggtggcgccc ggcatccaca gcgtgctgat ggccacgatc tccatgctgg
     7921 ccaagcccgg ccagacgatc tgcgtcgaat cgctctgcta ccccggcatc aaggccatgg
     7981 ccgcgcagtt caatgtcaag ctgcaagccc tgcccatgga cgaccagggc gtcgaccccg
     8041 atgccttgga gcatgtctgc aagacccagc gccccaaggc gctgatctgc aatcccacca
     8101 tccacaaccc cacgacggcc accatgggca aggagcggcg cgaagccatc gccgacatcg
     8161 cgctgcgcta cagcatcccc atcgtcgaag acgatgccta cgccaagctc ggggacgagg
     8221 acctgcccac gctgagcagc cttgcgcccg agctgaccta ctacgtcggc ggcttgtcca
     8281 agagcctggg agccggtttg cgcatcgcct acctccacgc gcccaccgac ctgcagaccc
     8341 agcggctggc aggcgcgctg cgcgccacca ccgtcatggc ctcgcccgtc acggccgccc
     8401 tggccacgcg ctggatcgtc tcgggcctgg cctaccgcat gctcgacgaa gtacgcaacg
     8461 aatcgatcgc gcgccaggcc ctggccgccc gcatcctgga cgatttcgac gtgcaggcgc
     8521 atcccgacgg cttccatcta tggatcaagg tcgacggcaa ccccgttgaa ctggccctgt
     8581 ccctgcgcgc ccgcggcgtt ccctgcgtgg ccagcgtcgc gttctcgaca gacgccgcgc
     8641 cacccgccgc gatccggcta tgcctgggcg gcaacctctc ccgcgccgag gcccagcaat
     8701 cgttgcacct ggtggccacg gccttgcgcc aggccgccgg cccggacgaa cacgcgcttt
     8761 gagtccggcg ggggcgctgt ttccagctac ttcgccggcc gcgccgcggc ggacgattcg
     8821 cgccgtgcca gccagagata cagcccgctg gcgagcacga cgatggtgat gaggtcgagc
     8881 agcgcccaga ggatcttgag cggcatgccg ccgtaatcgc cgaagtgcaa cggccgcgac
     8941 acctccagcg cccgcaggta ccagggcatc ttcacgaccg cggccagttc gccggtgcgc
     9001 gcatccacca ggacggggct gaacaggcgg ctggtcagcg gcgtatcgcc cttggcccag
     9061 agcaggtagt gataggggct gccgaagggc gagccgggaa acgtgacgct ggtcaccacc
     9121 atgcccggca gcgccagccg ggcggtgccg aaggcggcgt ccaccggcgt ccagtcggcc
     9181 tgggacgggg cgggcttgcc atgccatggc gcgagcatgg cctgcacgtc ggtctgccgc
     9241 cagagcgcga agagcggggt ggacagctcg ttcatgaggc cggtcacgcc cacccccagc
     9301 gtccaggcga gcgtggtcac gcccagcagg ttgtgccggt cgagccagcg gatccgcgcc
     9361 gagcggccgg cgcgcacggt gccgaagtcg agtttgcggg tgaaggggcc gtagagcacg
     9421 atgcccgaga cgatcgccag cacgaacagc agggccatca cgcccaggaa cagctcgccg
     9481 ggcaggtccg cgaacagatc acgatgcaaa tgcagcatca ggccgagaaa ggtctgcctt
     9541 tgctggccgg gcggcttgga ctgctccagc accgcgccgg tgtgggcgtc gaagcgcacg
     9601 a
//
LOCUS       DASWPO010000301         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1053329, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000301 DASWPO010000000
VERSION     DASWPO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053329"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1764)
                     /locus_tag="VGC69_15880"
     CDS             complement(10..1764)
                     /locus_tag="VGC69_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0296807.1"
                     /translation="MEFGFNLNRTANASAWRLLPNGWDFVAFPLIICVIAMAAVGFHQ
                     TLAPMSTLHDKAISLAPSNLPEYALRTTLRMLAAMAASLLFTLVYGTLAAKSRRAEKV
                     LVPILDILQSVPVLGYISFTVTFFLALFPSRVLGAELAAIFAIWTSQAWNMTFSFYQS
                     LRTVPRDLDEVSRSFHLTAWQRFWKLEVPFAMPGLIWNMMMSMSGGWFFVVASESITV
                     GNQSIALPGMGSYLAQAIAEKDLHAVGWVILVMVVVILAYDQLLFRPLVAWADKFRME
                     NTSSGNAPSSWLLSLIRRTRLIRMLLVPAAVLISKAARLPMRLSTPTVRRSAPARKKG
                     GSSLAGDLAWGTVIVLLTIYVAYRVVSYVSTGVSWGEVGHVFVLGFITLLRVVLLIAL
                     SSLVWVPLGVLIGLRPALAEKFQPLAQFLAAFPANLMFPVFVIVIVRFHLNPDIWLSP
                     LIVLGTQWYILFNVIAGASAYPNDYREAAANFHIRGWQWWRKVMLPGIFPYYVTGALT
                     ASGGSWNAAIVAEYVKWGDDKVSAHGIGAYIAQATEAGDFPRIVLGVAVMSVFVILFN
                     RLFWRPLFGFAERRLRLN"
BASE COUNT          467 a          697 c          704 g          323 t
ORIGIN      
        1 ctcgctgagt cagttgagcc gcagccggcg ctcggcaaag ccgaacaacg gccgccagaa
       61 caggcggttg aacaggatca cgaagacgct catgacggcg acgccgagca cgatccgcgg
      121 gaagtcgccc gcctcggtcg cctgggcgat ataggcaccg atgccgtgcg ccgacacctt
      181 gtcgtcaccc catttcacat attcggccac gatggcggcg ttccacgaac cgccggaggc
      241 cgtgagcgcg cccgtgacgt aataggggaa gatgcccggc agcatgacct tgcgccacca
      301 ctgccagccg cggatgtgga agttggcagc cgcttcgcgg tagtcgttgg gataggccga
      361 ggcccccgcg atcacgttga acaggatgta ccactgcgtg cccagcacga tgagcggcga
      421 cagccagatg tccgggttga ggtgaaagcg cacgatcacg atcacgaaga ccgggaacat
      481 caggttggcc gggaaggcgg ccaggaactg cgccaggggc tggaacttct cggccagcgc
      541 cgggcgcagg ccgatcagca cgcccagcgg cacccagacc agcgacgaca gcgcgatcag
      601 cagcaccacg cgcaagagcg tgatgaagcc cagcacgaac acgtggccga cctcgcccca
      661 gctcacgccc gtgctcacat agctcaccac ccggtaggcc acgtagatcg tcagcaagac
      721 gatgaccgtg ccccaggcca ggtcgccggc cagcgacgag ccgcccttct tgcgggctgg
      781 cgccgacctg cgcaccgtgg gggtcgacag gcgcatgggc aggcgcgccg ccttcgagat
      841 caggacggcg gccggcacca gcagcatgcg gatgaggcgc gtgcggcgga tgaggctgag
      901 cagccacgag ctgggcgcgt tgcccgaact ggtgttttcc atgcggaact tgtcggccca
      961 ggccacgagc ggacggaaca gcaattggtc gtaggccagg atcaccacca ccatgaccag
     1021 gatcacccag cccacggcgt gcaggtcctt ctcggcgatg gcctgcgcca ggtaggagcc
     1081 catgccgggc agcgcgatgc tctggttgcc caccgtgatc gactccgaag ccaccacgaa
     1141 gaaccagccg cccgacatgg acatcatcat gttccagatc aggccgggca tggcgaacgg
     1201 cacttccagc ttccagaagc gctgccaggc ggtcaggtga aagctgcgcg agacttcgtc
     1261 gaggtcgcgc ggcaccgtgc gcagcgactg gtagaagctg aaggtcatgt tccaggcctg
     1321 gctggtccag atggcgaaga tggcggccag ctccgcgccc agcacgcgcg aagggaacag
     1381 ggccaggaag aaggtgaccg tgaacgagat atagcccagc accggcaccg actgcaggat
     1441 gtcgaggatg gggaccagca ccttctcggc gcgccggctc ttggcggcca gggtgccgta
     1501 gaccagcgtg aacaggagcg aggccgccat ggccgccagc atgcgcaggg tggtccgcag
     1561 cgcgtactcg ggcaggttgg acggcgccag cgagatggcc ttgtcatgca gcgtcgacat
     1621 gggcgccagg gtctggtgga accccacggc ggccatggcg atcacgcaga tgatcagcgg
     1681 aaaggcgacg aaatcccagc cattgggcag caggcgccac gccgacgcgt tggctgtccg
     1741 gttgagattg aagccgaatt ccattgattt ctccgcacga ttttcaatcg gcccgccctg
     1801 ccccggccct gggccaagcc gggcaaggcc gccatcatca ccgcgtcata tgacacgaat
     1861 aggtatcccg tggcgcaaaa gccagggacg gcacagtcgg catgatagtc tgccggcccg
     1921 ccaccggcga ctgacgcgca agccttgcag gctgcgcaag gctaccgggt caagctttct
     1981 tcccgctttc tttttcgccg caaaagaaaa aggccgactg catggcagtt ggccttcgca
     2041 attccgggta agccgaacaa gattcggact cgtagtcaac aggattaaaa gacccgacaa
     2101 cccatggttc agcaaaattc accgaggcta cacgagatca acaatctcat taaaaatcaa
     2161 atagttatta atgaattttg ttttcgtcac c
//
LOCUS       DASWPO010000302         8519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2839647, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000302 DASWPO010000000
VERSION     DASWPO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839647"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /gene="tex"
                     /locus_tag="VGC69_15885"
     CDS             <1..1725
                     /gene="tex"
                     /locus_tag="VGC69_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172756218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein
                     Tex"
                     /protein_id="HEY0296808.1"
                     /translation="FRDWFDFSEPLRTLPSHRVLALLRGRQQGVLELRVGLQAELETE
                     TPHPCVARIASFLKLDGSALFSLDAKPRARWLGEVCRWTWRVKLLTAFESEFFGKLRD
                     EAEAEAIRVFAANLKDLLLAAPAGPKAVLGLDPGIRTGCKIAVIDRTGKVVDTTTVYP
                     FEPRRDVEGTINTLAALCAKHKVDLVAIGNGTASRESEKLVGEMMARFKDLTLTRVVV
                     SEAGASVYSASETAAAEFPNLDVTLRGAVSIARRLQDPLAELVKIDPKAIGVGQYQHD
                     LNQRELARSLDAVVEDCVNAVGVDVNTASAALLARVSGLNTLLAKNIVAWRDEHGAFP
                     SRAKLLDVPRFGDKAFEQAAGFLRIPNGANPLDASAVHPEAYPVVERILARVQKDMKQ
                     IIGQREALKGMSPSDFTDERFGLPTVRDIFSELEKPGRDPRPEFKTAQFREGVETLKD
                     LVPGMVLEGVVTNVANFGAFVDVGVHQDGLVHISALSEKFVKDPRDVVRVGQTVAVKV
                     LEVDVARQRVALTMRLNDAAEPVRRGTAGSAPAPRGGQGQRKPQGDAGRGAPTNSAMA
                     EAFAKLKR"
     gene            complement(1792..2052)
                     /locus_tag="VGC69_15890"
     CDS             complement(1792..2052)
                     /locus_tag="VGC69_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HEY0296809.1"
                     /translation="MSPVMPAFDTLKYANALKDVGIPDRQAEAQALALSGAFDVSLER
                     LATKDDLNLLATELRGENKITKWMLGTLIVAVGAMLKQMYFH"
     gene            complement(2221..3543)
                     /locus_tag="VGC69_15895"
     CDS             complement(2221..3543)
                     /locus_tag="VGC69_15895"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0296810.1"
                     /translation="MNTRVLTGITTTGTPHLGNYAGAIRPAVRASTQPGVDAFFFLAD
                     YHALIKCDDPARVARSRLELAATWLACGLDPERVTFYRQSDIPEIPELTWLLTCVTPK
                     GLMNRAHAYKASVDANAEKGIDPDDGVTMGLFSYPVLMAADIVMFNAHKVPVGRDQVQ
                     HLEMARDIAQRFNHLYGDDFFVLPEVQIEEDVATLPGLDGRKMSKSYNNTIPLFEGGA
                     AALRAAIMRIVTDSKQPGEPKDAEASHLYTLYKAFATPAENAAFRKQLEEGIGWGDAK
                     QALYEKLEGEIAPMRERYEALIGNPAHLEEILLAGARKARALATPAIDRLREAVGLRN
                     LNAGAANTVTKAEKAAKGARFVSFREKDGSFRFRLMAADGQELLTSVPYADPKQAGAV
                     MKRLPAEATLSAEGSGFTVSLDGAVVASAAVNTGLEQARVALASLVQD"
     gene            3709..5118
                     /locus_tag="VGC69_15900"
     CDS             3709..5118
                     /locus_tag="VGC69_15900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0296811.1"
                     /translation="MHLNLLRQQDGRRYSTYFCIFVLTFVLCLIGAHTRLSAIELSVF
                     WPVNVIDAALFYRLRLVRNPWCHASAYAAMVAQDSLSQGWGIDALTINAANIVFIFVL
                     GELLSRSTPVNQRRADITGFFHTVLACLAAAALCAVAGALAQQASGFAVARFDQALLS
                     WFSEEFYTAMLCLPILLTLRGTFTVRLPAITWKNTLPLLSLVASLGLSTLVGPFAILV
                     LPLPALTWCAVTYPLWLVQLITLCTGCLQLIMAAGHLHDVYSRWGETFIHEIAILRIC
                     IAATIFSPLLMAINAGTIRQLNRRLIRQASYDFLTNTLSRYGLTEALNQYGASHAYRN
                     ATVNIMLIDVDHFKSINDNFGHVCGDDVLKRIAAIINHAAPTPGLVGRIGGEEFVVVC
                     FDYAPQTFYALADALRQTIADTPFDCGQQRVSITISIGIAHATEPGSDLESAVQRLFP
                     RADENLYTAKRQGRNCTVQ"
     gene            complement(5137..6231)
                     /gene="zapE"
                     /locus_tag="VGC69_15905"
     CDS             complement(5137..6231)
                     /gene="zapE"
                     /locus_tag="VGC69_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY0296812.1"
                     /translation="MNVQQYYEQALSERGYQPDAAQQRAVDRLQRYYDDWVRYKALRS
                     NALKKLLNRPEVPRGIYLWGGVGRGKSFLMDAFYATVPVVRKTRLHFHEFIRGVHREL
                     EEVKGQQDPLDEVARRISRRYRLICFDEFHVSDVADAMILYRLLLKLFEYGTSFVMTS
                     NYEPSTLYPDGLHRDRILPAIALIQKRMDVLNVDAGVDYRRRALEQVQCYHYPLDDKA
                     NAALQEAFRHLADTPPEPNPVLRIEHREIKALALAGTVVWFDFATLCGGPRSQNDYLE
                     LASRFNAVILSGVPRMGPRHQSEARRFTWLVDVFYDHKVKLIMSAECSPEEIYTEGAF
                     ANEFHRTVSRILEMQSREYLESERRHTVDL"
     gene            complement(6443..7870)
                     /gene="lpdA"
                     /locus_tag="VGC69_15910"
     CDS             complement(6443..7870)
                     /gene="lpdA"
                     /locus_tag="VGC69_15910"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0296813.1"
                     /translation="MAKQFDVVVIGAGPGGYVAAIRAAQLGMSVACIDAWQNGQGGPA
                     PGGTCTNVGCIPSKALLQSSEHYEQAGHHFAEHGIEIKGLGLKLDTLLGRKNSVVKQN
                     NDGILYLFKKNKVTFFHGKGAFAGTAEGGYAIKVTGTTDEDLVGKHIIVATGSSPREL
                     PGLPFDEKVVLSNDGALNIGAVPKTLGVIGAGVIGLEMGSVWRRLGAEVTILEAMPEF
                     LAVADQQVAKEALKVFTKQGLVIETGVKLGELKATAKNVTVPYTDAKGAEQKLVVDKL
                     IVSIGRIPYTGGLNAESVGLKLDERGFVAVDGECKASLPNVWAIGDVVRGPMLAHKAE
                     EEGVAVAERIAGQHGHVNFDTIPFVIYTSPEIAWVGKTEQQLKKEGREYKAGSFPFLA
                     NGRARALGDTTGFAKVIADAKTDEVLGVHIIGPMASELISEAVTIMEFKGAAEDIARI
                     CHAHPTLSEAVKEAALAVDKRALNF"
     gene            complement(7887..>8519)
                     /gene="sucB"
                     /locus_tag="VGC69_15915"
     CDS             complement(7887..>8519)
                     /gene="sucB"
                     /locus_tag="VGC69_15915"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY0296814.1"
                     /translation="QAENAILTTFNEINMQAVIDLRNKYKEKFEKDHGVKLGFMSFFV
                     KAAVAALKKYPLINASIDGKDIIYHGYFDIGIAVGSPRGLVVPILRNADQLSIADIEK
                     AIGDFGRRAADGKLGIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERP
                     VVENGQIVIRPINYLALSYDHRIIDGREAVLGLVAMKEALEDPQRLLLDL"
BASE COUNT         1449 a         2857 c         2692 g         1521 t
ORIGIN      
        1 ttccgcgact ggttcgattt cagcgaaccg ctgcgcaccc tgccctcgca ccgcgtgctg
       61 gcgctgctgc gcggccgcca gcaaggcgtg ctggaactgc gcgtgggcct gcaggccgaa
      121 ctcgagaccg agacgcccca cccttgcgtg gcgcgcatcg ccagcttcct gaagctcgat
      181 ggaagcgccc tcttttccct ggacgccaaa ccgcgcgcgc gctggctggg cgaggtctgc
      241 cgctggacct ggcgcgtgaa gctgctcacg gccttcgaga gcgagttctt cggcaagctg
      301 cgcgacgagg ccgaggccga ggccatccgc gtcttcgccg ccaacctcaa ggacctgctg
      361 ctggccgccc ccgccggccc caaggccgtg ctgggcctgg acccgggcat ccgcaccggc
      421 tgcaagatcg ccgtcatcga ccgcaccggc aaggtggtcg acaccaccac cgtctacccc
      481 ttcgagccgc gccgcgacgt cgaaggcacg atcaacaccc tggcggcgct gtgcgccaag
      541 cataaggtag acctggtggc catcggcaac ggcaccgcct cgcgcgaaag cgaaaagctc
      601 gtgggcgaga tgatggcgcg cttcaaggac ctgacgctca cccgcgtggt cgtgtccgag
      661 gccggcgcct cggtctattc ggcttcggaa accgccgccg ccgaatttcc caacctcgac
      721 gtcaccctgc gcggcgccgt gtccattgcc cgccgcctgc aggacccgct ggccgaactg
      781 gtgaagatcg atcccaaggc catcggcgtg ggccagtacc agcacgacct caaccagcgc
      841 gagctggccc gctcgctgga cgccgtggtc gaggactgcg tcaacgccgt gggcgtggac
      901 gtcaataccg cctcggccgc gctgctggcg cgcgtctcgg gcctgaacac cctgctggcc
      961 aagaacatcg tggcctggcg cgacgagcac ggcgccttcc cctcgcgcgc caagctgctg
     1021 gacgtgccgc gcttcggcga caaggccttc gagcaggccg cgggcttcct gcgcatcccc
     1081 aacggcgcca acccgctcga cgcctcggcc gtgcaccccg aagcctaccc ggtggtcgaa
     1141 cgcatcctgg cccgcgtgca aaaggacatg aagcagatca tcggccagcg cgaagccctc
     1201 aaaggcatgt cgccgtccga tttcaccgac gaacgcttcg gcctgcccac cgtgcgcgac
     1261 atcttcagcg agctcgagaa acccggccgc gatccgcgcc ccgagttcaa gaccgcccag
     1321 ttcagggaag gcgtcgaaac gctgaaggac ctcgtgcccg gcatggtgct cgaaggcgtg
     1381 gtgaccaatg tggccaactt cggcgccttc gtcgacgtgg gcgtgcacca ggacggcctg
     1441 gtgcacatct cggccctgtc cgagaaattc gtcaaggacc cgcgcgacgt ggtgcgcgtc
     1501 ggccagaccg tggccgtgaa agtgctggaa gtcgacgtgg cccgccagcg cgtggcgctg
     1561 accatgcggc taaacgatgc cgcggagcct gtgcggcgcg gcacggctgg cagcgctcct
     1621 gccccgcgcg gcggccaagg ccagcgcaag ccgcaaggcg acgccggccg cggcgcgccg
     1681 acaaacagcg ccatggccga agcatttgcg aaattgaagc ggtaaaaccg aagcgatccg
     1741 gatcgatggg acatccagcc aataaaatgc tggatgtccc atcatgttca atcagtggaa
     1801 gtacatctgc ttcagcatgg cacccactgc aacgatgagc gtgcccagca tccacttcgt
     1861 gatcttgttt tccccgcgca gctccgtggc caacagattc agatcatctt tcgtggccag
     1921 gcgctcgaga ctgacgtcaa atgcgcctga caacgccaaa gcctgagcct cggcctgccg
     1981 gtcgggaata ccgacgtctt tcagagcatt ggcgtatttg agcgtatcga aggcaggcat
     2041 gacaggcgac atgaaaatcc ttgatgtaac caaaaaaagg caccaaggaa agtctactaa
     2101 agaacgcatc caccatacgg gtaccgtgca tgcatttgcc acccggggaa tctaccgatg
     2161 ccttttgacc cgtttcccct cttgccaaaa gccttaacta tctgctcgta acgcagaaaa
     2221 tcaatcctgg acgagcgaag ccaaggccac gcgtgcctgc tcaagccccg tgttcacggc
     2281 agccgaagcc accacggcac cgtcgagtga aaccgtaaag cccgagcctt ctgccgacaa
     2341 ggtcgcctcg gccggcaggc gcttcatcac ggcgcccgcc tgcttgggat cggcatacgg
     2401 cactgaagtg agcagttcct gcccatcggc cgccatcaga cggaagcgga agctgccgtc
     2461 cttctcgcgg aaactcacga aacgcgcgcc cttggcggcc ttctccgcct tggtgaccgt
     2521 attcgccgcg cccgcattca ggttgcgcag accgacagcc tcgcgcaggc gatcgatggc
     2581 cggcgtcgcc aacgcgcgcg ccttgcgggc gccagccagc aggatttcct cgaggtgggc
     2641 cggattgccg atcagggctt cgtatcgttc ccgcatgggc gcgatctcgc cctcgagctt
     2701 ctcgtacaag gcctgcttgg catcgcccca gccaatacct tcttccaact gcttgcgaaa
     2761 ggcggcgttc tcggccggcg tcgcaaacgc cttgtaaagc gtatagagat gcgaagcctc
     2821 ggcatccttg ggttcgcctg gctgcttcga atccgtcacg atgcgcatga tggccgcgcg
     2881 cagcgccgcg gcgccgccct cgaacagcgg aatcgtgttg ttgtagctct tggacatctt
     2941 gcgcccgtcc aggccgggca gcgtggccac atcttcctcg atctgcacct cgggcagcac
     3001 gaagaaatcg tcgccataca gatgattgaa ccgctgcgcg atatcgcgcg ccatctccag
     3061 gtgctgcacc tggtcgcggc ccacgggcac cttgtgcgcg ttgaacatca cgatgtcggc
     3121 ggccatcagc accggatacg aaaacaggcc catcgtgacc ccatcatcgg gatcgatgcc
     3181 tttctccgca ttggcatcca ccgaggcctt gtacgcatgc gcgcgattca tcaggccctt
     3241 gggcgtgacg caggtcagca gccaggtcag ctccggaatt tcgggaatgt ccgattgccg
     3301 gtagaaagtc acgcgctccg gatccaggcc gcaagccagc caggtcgccg ccagttccag
     3361 gcgcgagcgc gccacgcggg cgggatcgtc gcacttgatg agcgcgtggt agtcggccag
     3421 gaagaagaag gcgtccacgc ctggctgggt gctggcccgc accgccgggc ggatggcgcc
     3481 agcgtagttg cccaggtgcg gggtgcccgt ggtggtgatg ccggtgagaa cgcgggtatt
     3541 catgaaggca agcgtcttgt ttgtaagaat gccgaagagt ttagcgcgac acggcaaacc
     3601 gggctggaat ggcccgacca tgccgggtgc ttgcgctaag attcgcccgg gcccggaacc
     3661 gcgcgcggcc cggcgcgggc cgcctctcct cgaaacccgg catgcaatat gcacctgaat
     3721 cttcttcgcc agcaagacgg caggcgatat tccacctatt tctgcatttt cgtcctgaca
     3781 ttcgtgctct gcctgatcgg cgcgcatacc cggctgtcgg ccatcgagct gtcggtcttc
     3841 tggccggtca atgtcatcga cgccgccctg ttctaccggc tgcgcctcgt ccgcaacccc
     3901 tggtgccatg cgtccgccta cgccgccatg gtcgcccagg acagcctatc ccagggctgg
     3961 ggcatcgacg cattgacgat caatgccgcc aatatcgtct tcatcttcgt cctgggagag
     4021 cttctgtccc gctccacgcc cgtcaaccag cgccgggcgg acatcacggg gttcttccac
     4081 acggtcctcg cctgcctggc cgccgcggcg ctctgcgccg tggcgggcgc gctggcgcaa
     4141 caggcctccg ggtttgccgt ggcgcgattc gaccaggccc tgctcagctg gttcagcgag
     4201 gagttctaca cggcgatgct gtgcctgccc attctgctga cgctgcgcgg cacattcacc
     4261 gtccgcctgc ccgcgatcac ctggaagaac acgctgccgc tgctgtccct ggtggcctcg
     4321 ctgggcctga gcacgctggt cggtccgttc gccatcctgg tgctgccgct gccggcgctg
     4381 acctggtgcg ccgtcaccta ccccctgtgg ctggtccagc tcatcaccct gtgcaccggc
     4441 tgcctgcaac tgatcatggc cgcgggccac ctgcacgacg tctacagcag gtggggagag
     4501 accttcatcc acgagatcgc catcctgcgc atctgcatcg cggcgacgat cttcagcccc
     4561 ctgctcatgg ccatcaacgc gggaaccatc cggcagctca accgccggct gatacggcag
     4621 gccagctacg acttcctgac caacaccctg tcccgctacg gcctgaccga ggccctgaac
     4681 caatacggcg cgagccacgc ctaccggaat gccaccgtca acatcatgct gatcgacgtc
     4741 gaccacttca aaagcatcaa cgacaacttc ggccatgtct gcggcgacga cgtgctcaag
     4801 cgcatcgccg ccatcatcaa ccacgcggcc ccgacgccgg gcctggtcgg ccggatcggc
     4861 ggcgaggagt tcgtggtcgt ctgcttcgat tatgcgccgc agaccttcta tgccctggcg
     4921 gacgcgctgc gccagacgat cgccgacacg ccgttcgact gcggccagca gcgcgtgtcc
     4981 atcaccatca gcatcggcat cgcccatgcc accgaaccgg ggagcgacct ggaaagcgcc
     5041 gtgcagcgcc tgtttccccg cgccgacgag aacctctaca cggccaagcg gcagggacgc
     5101 aactgcaccg tgcaatgagc gtggccggcc ggccagtcat aggtcgacgg tgtgccgccg
     5161 ctcggattcc aggtattcgc gcgactgcat ctccaggatg cgcgacaccg tccggtggaa
     5221 ctcgttggcg aaggcgccct cggtatagat ttcctcgggc gaacactcgg ccgacatgat
     5281 gagcttgacc ttgtggtcgt agaacacatc caccagccag gtaaagcggc gcgcttcgga
     5341 ctggtggcgc ggccccatgc gcggcacccc cgacaggatc accgcattga agcggctggc
     5401 cagctccagg taatcgttct gcgaacgcgg tccgccgcac agcgtggcga aatcgaacca
     5461 caccaccgtg ccggccaagg ccagcgcctt gatctcgcga tgctcgatgc gcagcaccgg
     5521 gttcggctcg ggcggcgtgt cggccaaatg gcgaaaggcc tcctgcagcg ccgcgttcgc
     5581 cttgtcgtcc agcggatagt gatagcactg cacctgctcc agcgcgcggc gccggtagtc
     5641 gacgccggca tccacgttga gcacgtccat gcgcttctgg atcagtgcga tggcgggcag
     5701 gatgcgatcg cggtgcaggc cgtccggata caaggtggac ggttcgtagt tcgacgtcat
     5761 cacgaacgac gtgccgtact cgaacagctt gagcagcagg cggtacagga tcatggcgtc
     5821 ggccacgtcc gacacgtgga actcgtcgaa gcagatcagc cggtagcggc gcgatatgcg
     5881 gcgcgcgact tcgtcgagcg ggtcctgctg ccctttgact tcttcgagtt cgcggtgcac
     5941 gccgcgtatg aactcatgga agtgcaggcg cgtcttgcgc accaccggca cggtggcgta
     6001 gaaggcgtcc atgaggaagc tcttgccgcg ccccaccccg ccccacaggt agatgccgcg
     6061 cggcacctcg gggcggttga gcagtttctt cagggcgttg ctgcgcaggg ccttgtagcg
     6121 cacccagtcg tcgtaatagc gctgcaagcg gtcgacggcg cgctgctggg ctgcgtcggg
     6181 ctggtagccg cgctcggaca gggcctgctc gtaatattgt tggacgttca tgcgggggta
     6241 gataggcaat gggcggttct ctcgtcgtag agaagaaccg cccagattct ggttcttgag
     6301 ccgccgaggc tgccctgcgg aattccgatg ctcgcccccc aatgggggct gcgcttcgga
     6361 ttcctccggg gtcctcggct catggaaccg tcgagcgaac ggctctgcct cgaagaagca
     6421 ggtccgtccg cccgtaccag catcagaagt tcagcgcgcg cttgtccacc gccagggcgg
     6481 cttccttcac cgcttcggac agcgtggggt gcgcatggca gatgcgggcg atgtcttcgg
     6541 cggcgccctt gaattccatg atggtcacgg cctcggaaat cagctcggaa gccatgggac
     6601 cgatgatgtg cacgcccagg acctcatcgg tcttggcgtc ggcgatcacc ttggcgaagc
     6661 ccgtggtgtc gcccagcgcg cgggcgcggc cgttggccag gaaggggaag gagccggcct
     6721 tgtactcgcg gccttccttc ttcaactgct gctcggtctt gcccacccag gcgatttccg
     6781 gcgaggtgta gatcacgaag gggatcgtgt cgaaattgac atggccgtgc tggccggcga
     6841 tgcgctcggc cacggcaacg ccttcttcct cggccttgtg cgccagcatc gggccgcgca
     6901 ccacgtcgcc gatggcccag acgttgggca ggctggcctt gcattcgccg tccacggcga
     6961 cgaagccgcg ttcgtcgagc ttcaggccca cggactcggc gttcaggccg ccggtgtagg
     7021 gaatgcggcc gatcgagacg atcagcttgt ccaccaccag cttctgctcg gcgcccttcg
     7081 cgtcggtgta gggcacggtg acgttcttgg cggtggcctt gagctcaccc agcttgacgc
     7141 cggtctcgat gaccaggccc tgcttggtga agactttcag ggcttccttg gccacctgct
     7201 ggtcggccac ggccaggaat tccggcatgg cttcgaggat ggtgacctcg gcgcccaggc
     7261 ggcgccacac gctgcccatc tccaggccga tcacgcctgc gccgatcacg cccagcgtct
     7321 tgggcacggc gccgatgttg agcgcgccgt cgttcgacag tacgaccttc tcgtcgaaag
     7381 gcaggccggg cagttcgcgc ggcgacgagc ccgtggccac gatgatgtgc ttgccgacca
     7441 ggtcttcgtc ggtggtgccg gtgaccttga tggcatagcc gccttcggcg gtgccggcaa
     7501 aggcgccctt gccatggaag aaggtgacct tgttcttctt gaacaggtac aggatgccgt
     7561 cgttgttctg cttgaccacg ctgttcttgc ggccgagcag cgtgtcgagc ttcaggccca
     7621 ggcccttgat ctcgatgccg tgctcggcga agtggtggcc ggcctgttcg tagtgctcgg
     7681 acgactgcag cagcgccttc gagggaatgc agcccacgtt ggtgcaggtg ccgccgggag
     7741 ccgggccgcc ctggccgttc tgccaggcgt cgatgcaagc cacggacatg ccgagctggg
     7801 cggcgcggat ggcggcgacg taaccgccag gaccggcgcc gatgaccact acgtcaaatt
     7861 gctttgccat gatgatgtgc tcccgatcac agatccagca gcaggcgctg cgggtcttcc
     7921 agggcttcct tcatggccac caggcccagc acggcttcgc ggccgtcgat gatgcggtgg
     7981 tcgtaggaca aggcgaggta gttgatcggg cggatcacga tctggccgtt ctcgaccacg
     8041 gggcgttcct tggtggcgtg gacgcccagg atggccgatt gcggcgggtt gatgatgggg
     8101 gtggacagca tcgagccgaa cacgccgccg ttggagatcg agaaagtgcc gccggtcatc
     8161 tcttcgatgc ccagcttgcc gtcggcggcg cggcggccga agtcgccgat ggccttttca
     8221 atgtcggcga tcgacagctg gtcggcattg cgcaggatcg gcaccaccag gccgcgcggg
     8281 ctgcccacgg cgatgccgat gtcgaagtag ccgtgataga tgatgtcctt gccgtcgatc
     8341 gaggcgttga tcagcgggta tttcttcagc gcggcgaccg cggcctttac gaagaaggac
     8401 atgaagccga gcttcacgcc atgatccttc tcgaactttt ccttgtactt gttgcgcagg
     8461 tcgataacgg cctgcatgtt gatttcgttg aacgtggtca gaatcgcgtt ttcggcttg
//
LOCUS       DASWPO010000303         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_964627, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000303 DASWPO010000000
VERSION     DASWPO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964627"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..733
                     /locus_tag="VGC69_15920"
     CDS             119..733
                     /locus_tag="VGC69_15920"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0296815.1"
                     /translation="MEFQDSFEIPAPPSEVMRRFTDVERISLCVPGLTMTGRDDDDGG
                     YLGVMTVAFGPKRLKFQGKVRCEFDLEHCKGTLTGGGAAAGRSASIQVRTGFSVEQAP
                     GATAAAPRSVVRIVSHADLQGVLAQFAGTGGIALGRQLMRDFARNLTDELSKEVVPAP
                     DSAAARTGSGDRPMPPPARSVSAFRLLLAVAVAKLKGLFHVGSR"
     gene            717..1910
                     /locus_tag="VGC69_15925"
     CDS             717..1910
                     /locus_tag="VGC69_15925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0296816.1"
                     /translation="MSVPASPAGGERIPRRLRGILSLDDFEAAARRRLPRPIFGYVHG
                     AAEGNHSVRENRAAFGDYGFSTRVLVDVSARSQQVALFGKTYASPFGIAPMGITGMSA
                     YRADIGLVCAARAAGIPAIMSGASTVTLEEMAQRAPGTWFQAYVQGDPSSFGPLLARV
                     AQAGFEVLVVTVDVPVSANRENNVRTGFSTPLRPGLRLAYDGITRPAWLFGTFLRTLL
                     RHGMPHFENTFAQRGAPLLSARAARDFSQRDNLTWDHLADIRRRWQGALVVKGILDVR
                     DAAEARRHGVDGIIVSNHGGRQLDGAVAPLRVLPQIAEAAGDMAVMMDSGIRRGTDVL
                     KALALGARCVFLGRPFNYAAAVGGEAGVAHAIRLLREEVDRDMAMLGLCHCGEARRDI
                     LVPRA"
     gene            2141..>2982
                     /locus_tag="VGC69_15930"
     CDS             2141..>2982
                     /locus_tag="VGC69_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0296817.1"
                     /translation="MSNPAPAFLGIDFGTSNSAMAMLGADGMAHPIALEGRATAMPTA
                     LFFNFEERSTHFGRDAMAQYLEGTEGRLMRSLKSLLGSALLQEKTAVHGDLVSFQDII
                     ARFLRELAARAGATPGQPPRRVVIGRPVHFVDGDAARDQQAETSLREAAEAAGLGEVA
                     FQMEPIAAAFDYERRVEREALVMIVDIGGGTSDFTVVRLGPERMARADRAGDILATNG
                     VHIGGTDFDQRLNLALAMPLLGRGHIGPQGREVPSRVFFDLSTWHLIHWLYAPQAIRE
                     AQALR"
BASE COUNT          401 a         1032 c         1047 g          502 t
ORIGIN      
        1 ggcctgttcg tcgtggatgc ccgggtttcg ccttcggtgc tcaccgacac gtacctgaag
       61 ctccacttcg gtatcgagaa ccaggccccc ctgctgcgcg ccgaggggca gtgacaccat
      121 ggaattccag gacagtttcg agatcccggc gccaccgtcg gaggtgatgc ggcgcttcac
      181 cgacgtcgag cgcatttccc tgtgcgtccc cggcctcacg atgaccggac gcgacgacga
      241 cgacggcggc tacctgggcg tgatgacggt cgccttcggg ccgaagcggc tcaagttcca
      301 gggcaaggtc cggtgcgagt tcgacttgga gcattgcaaa gggacgctga ccggtggcgg
      361 ggccgcagcg gggaggtcgg ccagcatcca ggtgcgcacg ggcttctccg tggagcaggc
      421 gcccggcgct accgccgccg cgccgcgcag cgtggtccgg atcgtgtcgc atgccgacct
      481 gcagggtgtg ctggcccagt tcgccggcac gggcggcatc gcgctggggc ggcagctgat
      541 gcgggacttc gcgcgcaacc tcaccgatga attgtcgaaa gaggtcgtgc ccgcgccgga
      601 ctccgccgct gcgcgaaccg ggagcggcga caggcccatg ccgccgcctg ccaggtcggt
      661 atcggccttc agactgctgc tggccgtggc cgtggcgaag ctcaagggcc ttttccatgt
      721 cggttcccgc tagccctgcg gggggcgagc ggataccgcg tcgcctgcgc ggcatcctgt
      781 cgctggacga tttcgaggcc gccgcccggc gccgcctgcc gcgcccgatc ttcggctacg
      841 tgcatggcgc cgccgaaggc aaccattcgg tgcgcgagaa ccgcgccgcg ttcggcgact
      901 acggtttctc gacccgcgtg ctggtcgatg tctcggcgcg cagccagcag gttgcgctgt
      961 tcggcaaaac ctatgcttcg ccgttcggca tcgcgcccat gggcatcacg ggcatgtcgg
     1021 cctaccgcgc tgacatcggc ctggtctgcg ccgcgcgggc ggccggaatc cccgccatca
     1081 tgagcggcgc atcgaccgtg acgctcgaag agatggcgca gcgcgcaccg ggcacgtggt
     1141 tccaggccta tgtgcagggc gatccgtcga gcttcggccc actgctggcg cgggtggcgc
     1201 aggcggggtt cgaggtgctg gtggtcacgg tggacgtgcc ggtgtcggcc aaccgcgaga
     1261 acaacgttcg cacgggcttc tcgacaccac tgcgcccggg cctgcggctc gcctacgacg
     1321 gcatcacgcg gcccgcgtgg ctgttcggta ccttcctgcg cacgctgctg cggcatggca
     1381 tgccgcactt cgagaacacg ttcgcccagc gcggcgcgcc gctgctgtcg gcccgggctg
     1441 cgcgcgactt ctcccagcgc gacaacctga cctgggacca cctggccgac atccggcgtc
     1501 gctggcaggg cgcgctggtc gtcaagggca tcctcgacgt gcgcgatgcg gcggaggccc
     1561 gccggcacgg cgtggacggc atcatcgtct ccaaccacgg cggccgccag ctcgatggcg
     1621 ccgtcgcgcc gctgcgcgtg ctgccgcaga tcgccgaggc ggccggcgac atggccgtga
     1681 tgatggacag cggcatccgc cgcggcaccg acgtgctcaa ggccctggcg ctcggtgcgc
     1741 gctgtgtttt cctgggccgg cctttcaact atgccgccgc cgtcggcggc gaggccggtg
     1801 tcgcgcacgc gatccgattg ctgcgtgagg aggtggatcg cgatatggcg atgctcggcc
     1861 tctgtcactg cggcgaggcc cgccgcgaca tcctcgtgcc tcgcgcctga cgggccctgc
     1921 tcgacggcat tgtgcggtct gtgcgcgcgt gcgcctcccg cgcattgcgg tcgcagcgtg
     1981 gcttatgatg cggcccgcac catcgtcgac atgcgctgtg catgctgcct ggtcgcgctc
     2041 ccgaagggca tcgtgacctt tctgcctcct ttcccaccgt gcgccgccag tccttgcatc
     2101 tccatggcgg cacgggcatt ccttctgcat ccaccggcac atgagcaatc ctgccccagc
     2161 tttccttggc atcgacttcg gcacttccaa ttccgccatg gcgatgctgg gtgctgacgg
     2221 catggcccac cccattgccc tcgagggccg cgccacggcc atgcccacgg cgctgttctt
     2281 caatttcgag gagcgcagca cgcacttcgg gcgcgacgcg atggcgcagt acctggaagg
     2341 caccgaggga cggctgatgc gctcgctcaa gagcctgttg ggcagcgcgc tgctgcagga
     2401 gaaaaccgcc gtgcatggcg atctggtgag cttccaggac atcattgcgc gctttctgcg
     2461 cgaattggcc gcgcgcgctg gagctacgcc agggcagccg ccccggcgcg tggtgatcgg
     2521 gcggccagtg catttcgtgg atggcgatgc ggcgcgtgac cagcaagccg aaacctcgct
     2581 gcgcgaggcc gcagaggcgg cgggcctggg cgaggtggcg ttccagatgg agcctattgc
     2641 cgccgcgttt gactatgagc gccgggtgga gcgcgaggcg ctggtgatga tcgtcgatat
     2701 cggcggtggc acctccgatt tcaccgtggt gcggctgggc cccgagcgca tggcgcgggc
     2761 cgaccgcgcg ggcgacatcc tggccaccaa cggcgtgcac atcggcggca ccgatttcga
     2821 ccagcggctc aacctggcgc tggcgatgcc gctgctgggg cgcggccaca tcggcccgca
     2881 ggggcgcgag gtgcccagcc gcgtgttctt cgacctttcc acctggcacc tgatccattg
     2941 gctctatgcg ccacaggcga tccgcgaggc gcaggcgctg cg
//
LOCUS       DASWPO010000304         4724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1859580, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000304 DASWPO010000000
VERSION     DASWPO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859580"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1045
                     /locus_tag="VGC69_15935"
     CDS             2..1045
                     /locus_tag="VGC69_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0296818.1"
                     /translation="MLSAPPAPVGIEQAAALLRSLYGLAGALAPLGGDRDANFLVTLD
                     AAAAQRTWILKISHPVEPAQVADFQTQALLHLERHAPDLPVQRLRRTAAGGHAALARL
                     GGAARVIRLFSYLDGLPMPQAPRSPRQMVNVARALAGLDIALAAMPAHAAGTLRLPWD
                     IQHADGVRSLVGLLGPAERQALAAHALDRFEGLVKPRLPGLRRQAIHNDFNLFNLLAD
                     PADPDRVCGILDFGDMVEGPTIDDVAVAASYHIDAGGDTLQAIVAFASAYHEINPLRP
                     EELDLLLDLIRARLAMVVCIGGWRAARDPSNAEYVLRNNAVSWARLAACHGIGDAAAR
                     EALRAACPSPPPG"
     gene            complement(1055..1993)
                     /locus_tag="VGC69_15940"
     CDS             complement(1055..1993)
                     /locus_tag="VGC69_15940"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296819.1"
                     /translation="MDSRLDLNLLLTLETLLIERSVTRAAAKLRSSQPAVSAQLARLR
                     RMFDDPLLIPGARAMTPTPRALEIAPRLSELVDGFRAIVQREDFDLSTAQDLVMISAS
                     DAVQCRIGKWFVTMADAAPGIQMGFVPGTRALIPALDSYMSSGQVDLLVGCSQMIHHD
                     RLHMRQLYQEHLVCVMRRDHPFRGDTLTPEMFSSTKHLIVSPLGGDFSGVTDRVLHKL
                     DLSRRGTVSVPSFLVAEQILCDTDLIAVFPATLARERAAVLKSYPLPMPAPTIEIAMT
                     WHERTHHSPLHRWLREQIASILTEWATGTPTAFSSA"
     gene            2133..2471
                     /locus_tag="VGC69_15945"
     CDS             2133..2471
                     /locus_tag="VGC69_15945"
                     /inference="COORDINATES: protein motif:HMM:NF028296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CzcE family metal-binding protein"
                     /protein_id="HEY0296820.1"
                     /translation="MKTQFLSILTVSAALGLASMSTSAVAAPSDSLLGTVAPSAQADR
                     TISLTGGTNDVLVNWGETVNFVAANGQQFTVKFDGVRNAFNLQDLVPASALDHGVEVY
                     VHSPLSSIGA"
     gene            complement(2613..3242)
                     /locus_tag="VGC69_15950"
     CDS             complement(2613..3242)
                     /locus_tag="VGC69_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0296821.1"
                     /translation="MTDLLNMILHIDQTLGVWVAQYGAWVYLVLFLIVFAETGLIVMA
                     FLPGDSLLFIGGAFSAAGSMHAGLLIVLLLVAAVLGNTVNFFIGRAVGKQAYTANWRF
                     LDHRALRRTHAFYEKHGGKALVLARFIPVIRAFAPFVAGVAGMRLASFQAFNVIGALL
                     WVVLLVMAGYFFGNIPFIKTHLNAIVLVGLAAAVVPVGLGVLARLMRRA"
     gene            3366..>4724
                     /gene="mutL"
                     /locus_tag="VGC69_15955"
     CDS             3366..>4724
                     /gene="mutL"
                     /locus_tag="VGC69_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY0296822.1"
                     /translation="MTDRRPIAALPDLLISQIAAGEVIERPASVLKELLENAVDAGAR
                     AVEVRLDGGGIKRIAVTDDGGGIPPEELPLALMRHATSKIRDLAELESVASMGFRGEA
                     LASIASVARLALISRTREADHAWQIEAGSLQLAPASGPVGTTVDVRQLFDSVPARRKF
                     LRAEATEFGHCLDVLERIALAHPGVAFRLFHNERAQRQWLPADPGQRIRDVLGEEFTG
                     QALPLQQAAGAIALAGMVSRPTAARARADRQYLYVNGRYVRDRTVSHALRAAYADVLH
                     GDRQPAYVLYLDIDPGAVDVNVHPAKHEVRFRDSGAVHRFVNQIVAQALAQTGGAQTA
                     ADPRPAPMPAAVDADARPAPPAQRPQAQVPFRLHEPAGIPVRDWQSFYRPQGDAAAAA
                     TAVADHATLPPSYAAPASAIAPALREATPQPLPEGEFPLGMALGQLHGIYILAQNTRG
                     LVL"
BASE COUNT          778 a         1737 c         1510 g          699 t
ORIGIN      
        1 ggtgctgagc gcgccgcccg cgcccgtcgg tatcgaacag gccgccgcgc tgctgcgctc
       61 gctgtacggc ctggcaggcg cgctcgcgcc gctgggcggc gaccgcgacg ccaacttcct
      121 ggtcacgctc gacgccgccg cggcgcagcg cacctggatc ctgaagatct cgcacccggt
      181 cgaaccggcg caagtcgccg acttccagac ccaggcgcta ttgcacctgg aacgccacgc
      241 ccccgatctg ccggtgcagc gcctgcgccg caccgccgcg ggcggccacg cggcgctggc
      301 gcggctcggc ggcgcggcgc gcgtgatccg gctgttcagc tacctggacg gcctgcccat
      361 gccccaggcg ccccgctcgc cgcgccagat ggtcaacgtg gcgcgcgccc tggccgggct
      421 ggatatcgcg ctggccgcca tgccggcgca tgccgcgggc accctccggc tgccctggga
      481 tatccagcac gccgacggcg tgcgcagcct ggtcggcctg ctcggcccgg ccgaacgcca
      541 ggccctggcc gcgcacgccc tcgaccgctt cgagggcctc gtcaagcccc ggctgcccgg
      601 actgcggcgc caggccatcc acaacgattt caaccttttc aacctgctgg ccgatccggc
      661 cgaccccgac agggtctgcg gcatcctgga tttcggcgac atggtggaag ggccgaccat
      721 cgacgatgtc gcggtcgcgg cctcctacca catcgatgcc ggcggcgaca cgctgcaggc
      781 catcgtggcc ttcgcttcgg cctaccacga gatcaacccg ctgcgccccg aagaactcga
      841 cctgctgctc gacctgatcc gcgcgcgcct ggccatggtg gtgtgcatcg gcggctggcg
      901 cgccgcgcgc gacccgtcca acgccgagta cgtgctgcgc aacaacgcgg tgtcctgggc
      961 gcgcctggcc gcctgccacg gcatcggcga cgccgccgcg cgcgaggcgc tgcgcgccgc
     1021 ctgcccaagc ccgccgccag gctgaagccg ggcctcaggc cgacgaaaag gcggttggcg
     1081 ttcccgttgc ccactcggtc agtatgcttg cgatctgttc gcgcagccag cgatgcagcg
     1141 ggctgtgatg agtgcgctca tgccacgtca tggcgatttc gatggtcgga gcgggcatcg
     1201 gcagcgggta ggacttgagc accgccgccc gttcgcgcgc caacgtcgcc gggaagaccg
     1261 cgatgagatc ggtgtcgcac aggatttgct cggccaccag aaagctgggc accgacaccg
     1321 tgccgcggcg cgatagatcc agtttgtgca gcacgcggtc ggtaaccccg gaaaaatccc
     1381 cgcccagcgg agacacgatc aggtgtttcg tggatgaaaa catctccggg gtgagcgtgt
     1441 cgccgcggaa cggatggtcg cgccgcatga cgcataccag atgttcctgg tagagctggc
     1501 gcatgtgcag gcggtcatgg tggatcatct ggctgcaccc gacgagaagg tccacctggc
     1561 ccgaagacat atagctgtcc agggccggga tcagggcgcg ggtgcccggc acgaatccca
     1621 tttgtatgcc cggcgcggcg tcggccatgg tcacgaacca tttgccgatg cggcattgga
     1681 cggcatcgct ggcgctgatc atgacaaggt cttgagcggt ggacagatcg aagtcttcgc
     1741 gctgcacgat ggcgcggaaa ccatcgacca gctccgacag cctgggggcg atctccagcg
     1801 cccgcggggt gggtgtcatg gcgcgtgcac ccgggatcag caaagggtcg tcgaacatgc
     1861 ggcgcaaacg tgccagctgt gcggaaaccg ccggctggct gctgcgcagc ttggccgccg
     1921 cccgcgtcac gctgcgttcg atcaacaagg tctccagcgt caggagcaga ttgaggtcaa
     1981 ggcggctatc cataatccaa atcttatatt aagaatatat gaaatttatt tgaattatag
     2041 attaggtggg cgcactattc attcacgcag ggattaagcg ttaaccctta aacgttaacc
     2101 cttgtttcgc aacccattct aggagcttca ccatgaaaac ccaattcctc tccatcctca
     2161 ccgtttcggc cgccctgggt ctggccagca tgtcaaccag cgccgtggcc gccccttccg
     2221 acagcctgct gggcactgtc gcgccctcgg cccaagccga ccgcaccatc tccctgaccg
     2281 gcggcaccaa cgacgttctc gtcaattggg gtgaaacggt gaacttcgtg gccgccaacg
     2341 gccagcagtt caccgtcaag ttcgacggcg tgcgcaacgc gttcaacctg caagacctgg
     2401 tccccgccag cgccctggac catggggtcg aagtctatgt gcacagcccc ctgagctcca
     2461 tcggcgcctg atcctcaagc aggacgtctc cctctggcgc ctgtcatcgg caccggggag
     2521 gaggcagacc ggcaggtccg cacgacttgt gtttttcgaa cgccgggcgc agtggcccgg
     2581 cttgttccgc aggcccaaga cggctgaagc gctcaagccc tgcgcatcag ccgggccagc
     2641 acccccagcc ccaccggcac cacggcggcg gccagcccca ccagcacgat cgcattcaag
     2701 tgcgtcttga tgaacgggat atttccgaag aaataccccg ccatcaccag caacaccacc
     2761 cagagcaggg ctccgatcac attgaatgcc tggaagctcg ccaagcgcat cccggccacg
     2821 cccgccacga atggcgcgaa ggcgcggatc accgggatga agcgcgccag caccagcgcc
     2881 ttgccgccgt gtttttcata gaacgcatgc gtgcgccgca gcgccctgtg gtcgagaaag
     2941 cgccagttgg cggtataggc ctgcttgccg accgcgcggc cgatgaagaa gttcaccgtg
     3001 ttgcccagca cggccgcgac cagcagcagc acgatcagga gccccgcgtg catgctgccc
     3061 gcggcgctga acgcgccgcc gatgaacagc agcgaatccc ccggcagaaa ggccatcacg
     3121 atcaggccgg tttcggcgaa cacgatcagg aacagcacca ggtacaccca ggccccgtac
     3181 tgggccaccc agacgcccag cgtctggtcg atgtgcagga tcatgttcaa caaatcggtc
     3241 atggcgatgc cgtggcaaaa aaagaagtta gggactatac cgtagggcgc gcgaccggcg
     3301 cccgctccgc gggccagtca gcttcctgcg gataaaatgg ctccaacccg tctgccgaac
     3361 ccgccatgac cgaccgccgc cccattgccg ccctgcccga cctgctgatc agccagatcg
     3421 ccgcgggcga agtcatcgag cggccggcct ccgtgctcaa ggagctgctc gagaacgccg
     3481 tcgacgccgg cgcgcgcgcc gtcgaagtgc ggctggacgg cggcggcatc aagcgcatcg
     3541 ccgtcaccga cgacggcggc ggcatcccgc ccgaggagct gcccctggcg ctgatgcgcc
     3601 atgccaccag caagatccgc gacctggccg aactggaatc ggtcgcgtcc atgggttttc
     3661 gcggcgaggc cctggcctcc atcgcctcgg tggcgcgcct ggccctcatc tcgcgcacgc
     3721 gcgaggccga ccatgcctgg cagatcgagg ccggcagcct gcagcttgcg ccggcgtccg
     3781 ggccggtggg caccacggtc gacgtgcggc aactgttcga ttccgtgccg gcgcgccgca
     3841 agttcctgcg cgccgaggcc accgaattcg gccactgcct ggacgtgctc gagcgcatcg
     3901 cgctggccca tcccggcgtc gccttccgcc tgttccacaa cgagcgcgcc cagcgccagt
     3961 ggctgccggc cgaccccggc cagcgcatcc gcgacgtgct gggcgaggaa ttcaccggcc
     4021 aggccctgcc gctgcagcag gccgcgggcg ccatcgccct ggccggcatg gtgtcgcggc
     4081 ccaccgccgc gcgcgcccgc gccgaccgcc agtacctgta cgtcaacggc cgctacgtgc
     4141 gcgaccgcac cgtcagccac gcgctgcgcg ccgcctatgc cgacgtgctg cacggcgacc
     4201 gccagcccgc ttacgtgctc tacctggaca tcgatccggg cgcggtcgac gtcaacgtgc
     4261 atcccgccaa gcacgaagtg cggtttcgcg acagcggcgc cgtgcatcgc ttcgtgaacc
     4321 agatcgtggc gcaggcgctg gcgcagaccg gcggcgcgca gaccgccgcc gatccgcggc
     4381 ccgcccccat gcccgccgcc gtcgatgccg acgcgcggcc cgccccgccg gcgcagcgcc
     4441 cgcaggcgca agtgcccttc cgcctgcacg agcccgccgg catccccgtg cgcgactggc
     4501 agtcgttcta tcgcccgcag ggcgacgccg cggccgcagc gaccgccgtc gcggaccacg
     4561 ccacgctgcc gcccagctat gccgcacccg cgtccgccat cgcgcccgcg ctgcgcgagg
     4621 caacgcccca gcccctgccg gaaggcgagt tcccgctggg catggccctg gggcagttgc
     4681 acggcatcta catcctggcg cagaacacgc gcggcctggt gctg
//
LOCUS       DASWPO010000305        17412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_71978, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000305 DASWPO010000000
VERSION     DASWPO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 17412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17412
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71978"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VGC69_15960"
     CDS             <1..755
                     /locus_tag="VGC69_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0296823.1"
                     /translation="TRGLLELMAETGHPLTLVTKNALVERDIDLLSDLARRNLVVVYV
                     SITTLDADMARTLEPRASAPWRRLQAVRTLAAAGVPVGVLAAPMIPFINDAFLESILA
                     EAASAGARWAGYTVVRLPWEVLAVFEQWLSTHFPDRAERVLHRIEDLRGGRRNDPRFG
                     TRMRGTGLWADLLRQRFSVAVRKQGLNLTRLQLDCQQFVAPAPDLSAGRAAERPVSFH
                     RGADDARGSSPIRTRSTRITSTSSVSQLSLFD"
     gene            821..1144
                     /locus_tag="VGC69_15965"
     CDS             821..1144
                     /locus_tag="VGC69_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296824.1"
                     /translation="MSIAASAVLSSLPLSGFGKPESGQAQPGKSRKPGASSPPRHEPA
                     AKAPSRKWPFPPAPQPAASLGDARKLAPNQIVSRREMLLDVLLVAAWGAIIPALMWLG
                     AAAGF"
     gene            1229..1699
                     /locus_tag="VGC69_15970"
     CDS             1229..1699
                     /locus_tag="VGC69_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0296825.1"
                     /translation="MSTAPVAQEAARARFGSLLSQAARSWRRAANRRLQPYGLTEATW
                     LPLIRLVRSPEPMRQKDLAASLGLDGSSVVRILDALESAGLVERRAEAGDRRAKAILL
                     TEQGQAVVAQVERVSQEVRDEALQGMSDSEVLQAHALLERICERLDTGDEAVSP"
     gene            1741..2943
                     /locus_tag="VGC69_15975"
     CDS             1741..2943
                     /locus_tag="VGC69_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY0296826.1"
                     /translation="MPGSATRVVPNVPLWLPALFTFSGTLAMHIFVPALAMAARDLNA
                     SSAAMQMTVSLYVLGLAGGQLIYGPLSDRYGRRPVLMVGLVVYTVAGLVCALAAQAHI
                     LVVARLFQALGGCSGLVLGRAIVRDTASTDNAAKRLALMNLMVVLAPALAPLIGSLVA
                     AAVGWRALLALLCLLGVVNFVLAWRKLPETHPGTSSLSLHNLKHEYRGLLGSRQFLGY
                     TVGGGCATTSMYAFIASAPFIFVEQMHRPDHEVGLYLTIMVLGAWLGNLLCGRLLGRI
                     PIGRLLLLGNTLSLVCIVLALAWALTGLMTVFVLLFLMFLYTVGAGVSSPAAMTLALS
                     VNPKVVGSASGVYGCVQMAVGALCTALAGLGSSPLLSAIVVMLVACAVAQLAMRMALG
                     GVGVRKAL"
     gene            3074..3463
                     /gene="rplS"
                     /locus_tag="VGC69_15980"
     CDS             3074..3463
                     /gene="rplS"
                     /locus_tag="VGC69_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY0296827.1"
                     /translation="MNLIAILEQEEIARLTGGKAMPDFGPGDTVVVNVNVVEGTRKRV
                     QAFEGVVIARRNRGLNSAFIVRKISSGEAVERTFQLYSPQIASIEIKRRGDVRRAKLY
                     YLRNRSGKSARIKEKLVTKTQRQAPQA"
     gene            3684..4403
                     /locus_tag="VGC69_15985"
     CDS             3684..4403
                     /locus_tag="VGC69_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY0296828.1"
                     /translation="MPDSAPSRPSNPATAGFDPMSQPWQAADQGLSAVGRQWFEPESF
                     RRLWAGITDWQQEDRPGTELRPNGREAAVLVPLVMRPEGLRVMLTQRTAHLHDHAGQI
                     SFPGGRIEAEDASPVDAALREALEETGLPQAHVEVLGTMPKYATSTGFAVTPVVGLVR
                     PDFTLAPDAFEVAEVFETPMEFLMNPANHRLYRATLPDGLQRQYFAMPWEGRFIWGAT
                     AGMLRNLYRNLYNAVLAAAHQ"
     gene            complement(4397..5317)
                     /gene="rsgA"
                     /locus_tag="VGC69_15990"
     CDS             complement(4397..5317)
                     /gene="rsgA"
                     /locus_tag="VGC69_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY0296829.1"
                     /translation="MSETTASLEGRIIAAHGRHYMVEFPDGTTRQCFPRGKKAGAAVG
                     DFVRISPQGRDEGAIDAVLPRRNLLYRSDDMRTKQFAANVDQLLIVVAVEPTFSDDLT
                     GRALAGAWSVGIDALIVLNKIDLQDGLPAARRRLATLSALGVPVIELIAHDADMVRKQ
                     LAARLAGKTSLLLGQSGMGKSTLLNTLAPEAHAATREYSAALDMGRHTTTSTQIYHLP
                     ALDDKPGGDLIDSPGFQAFGLQHLSRDDIVRGFPEFLPQIERCRFYNCSHRHEPGCGV
                     VAAVQAGLIAPERYALYLRILDEHEAPPRY"
     gene            complement(5314..6588)
                     /locus_tag="VGC69_15995"
     CDS             complement(5314..6588)
                     /locus_tag="VGC69_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0296830.1"
                     /translation="MSSPTRFALVFVVLLLADMAVRLWLSARQMRHVASHRDQVPAEF
                     AARISLSSHQRAASYTLARVRLGMVERVYDAVILVGLTLLGGLQWLDQAVGHLTGHAL
                     ARQLLLLVAVMAVMGVLGLPFTLWRQFRLEARFGFNRMTPALFVADAVKGIVLALALG
                     LPLAAAVLWLMAEAGQAWWLWAWGLWVAFNLLLLFIYPTFIAPLFNKFTPLDDDALAS
                     RIRQLAQRCGFALNGLFVMDGSRRSAHGNAYFTGFGKARRIVFFDTLLARLNGDEIEA
                     VLAHELGHFARGHIVKRIALSFVLALAFFALLGWLAQQPWFYAGLGVQARGDGGDNAL
                     ALLLFFLALPVFTFLLTPLGSWYSRSNEFEADRYAAQQSSAGQLVSALVKLYDDNAAT
                     LTPDPVHSAFYDSHPPAAVRIRHLMQASPHPA"
     gene            6678..7223
                     /gene="orn"
                     /locus_tag="VGC69_16000"
     CDS             6678..7223
                     /gene="orn"
                     /locus_tag="VGC69_16000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY0296831.1"
                     /translation="MAQHESRLVWLDMEMTGLDPEKERIIEVAVVVTEPDLTVVAEGP
                     VLVIHQPDSLLDAMDNWNKSTHGKSGLIDKVKASSLTEAQAEQQLIAFLAQYVPAGKS
                     PLCGNTISQDRRFMFRYMPGLERFFHYRNLDVSTLKELARRWAPAMLKGFEKKSRHEA
                     LADIYESIDELKYYREHFLKV"
     gene            complement(7189..8490)
                     /locus_tag="VGC69_16005"
     CDS             complement(7189..8490)
                     /locus_tag="VGC69_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEY0296832.1"
                     /translation="MLERPLSAPPGWRQFLADLNPTVAANGLIGLIFSATGPVAIVLS
                     VGTHGGLSEAQMASWLFGAFALNGLLSILMCWRYRQPLVCFWTIPGTVLIGPALGHLT
                     FPEVMGACYASGLLMLVLGLSGGVRRVMSAVPMPIVMGMVAGVFLRFGLDLVRSLHDN
                     LAIAGPMVATWLLLCAVPAWGRRVPPILGALIVGALAIYLSGQGGGMSLGGSHAVFVR
                     PVFETPVWSWPAMVELLVPLVVTSLIVQNGQGIAALKAAGHEPPINAVSVACGIGTLL
                     SAAVGCACTCLAGPSNAIITSSGRRGSHYAGGICCAVLAIAFGVLSPVFTRLLLHTPI
                     AFIMALAGLAMLRVLQGAFTTAFRTRFSLGALVAFLVTVADLGIANIGAAFWGLVAGF
                     VVSWLLERQDFRALADELAAQRMQGAAQAGATPSGSVRGST"
     gene            complement(8549..9484)
                     /gene="paaX"
                     /locus_tag="VGC69_16010"
     CDS             complement(8549..9484)
                     /gene="paaX"
                     /locus_tag="VGC69_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HEY0296833.1"
                     /translation="MAIAPSPLDRFLGRLLKADPPRAKSLCVSLLGDAVVPHGGAIWL
                     GGLIELLGPVGINERQLRTSVFRLVAQGWLQTERHGRRSLYLLSEQGRRDTAHASLRI
                     YEHVQPGWDGTWTLVALPRIGNNALAERAELRRELLWEGFGMVAPGLFAHPQTEAGAA
                     HEILERLGIPDKALVLSARDLAGAGSLPIASLAVQCWNLDEVAGLYRLFARNFAPLEK
                     LLEDEAATPAAAFAARVLLLHHWRRAVLHDPRLPTALLPASWPGHEARALCGRLYWRL
                     FDASEVHLDAIAGQDNPSYRPLQDEALARFGGRGG"
     gene            9659..10648
                     /gene="paaA"
                     /locus_tag="VGC69_16015"
     CDS             9659..10648
                     /gene="paaA"
                     /locus_tag="VGC69_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEY0296834.1"
                     /translation="MYAQLVETGVARVRGVDELSGPEQAFQRRLEDNVRIEPKDWMPE
                     AYRKTLVRQISQHAHSEIVGMLPEGNWITRAPSLKRKAILMAKVQDEGGHGLYLYSAA
                     ETLGVSRDALIDDLHAGRAKYSSIFNYPTLTWADVGMVGWLVDGSAIINQIPLCRCSY
                     GPYARAMVRVCKEESFHQRQGYDLLVQMCLHGTPEQKAMCQDALNRWWWPSLMMFGPP
                     DGDSPNSAQSMLWKIKLFSNDELRQKMVDQTVPQAEYLGLTVPDPDVKWNAERGHYDF
                     GEIDWSEFHAVLKGNGPCNRERLAARVKAHEAGAWVRDALVAHAEKKERRKAA"
     gene            10861..11142
                     /gene="paaB"
                     /locus_tag="VGC69_16020"
     CDS             10861..11142
                     /gene="paaB"
                     /locus_tag="VGC69_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEY0296835.1"
                     /translation="MSKEWPLWEVFIRSQHGLAHKHVGSLHAPDAEMAINNARDVYTR
                     RNEGLSIWVVRAADISASSPSDKEPLFEPANDKVYRHPTFFPMPDDVHM"
     gene            11160..11924
                     /gene="paaC"
                     /locus_tag="VGC69_16025"
     CDS             11160..11924
                     /gene="paaC"
                     /locus_tag="VGC69_16025"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY0296836.1"
                     /translation="MDKTLFQYLLRLGDGDLILSQRLGALIGHGPVLEEDMALANTAL
                     DLLGQARLWLTLAGEVEGAGRDEDALAYHRDAPQFLNPLLVERPNGDYAQTMTRQYFY
                     DVWHYYLLQGLAQSADARVAAIAAKGIKEVAYHVRRSSDLVVRLGDGTDESHARMQAA
                     VDELWRYTGELFTDDEIDRDVAARGIGCAMAPLREPWLAQVREVLAEATLAVPDEAQA
                     WHALLSGGRQGRHTEDLGYVLAEMQHLQRTYPGATW"
     gene            11957..12427
                     /gene="paaD"
                     /locus_tag="VGC69_16030"
     CDS             11957..12427
                     /gene="paaD"
                     /locus_tag="VGC69_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEY0296837.1"
                     /translation="MQWLQAVPDPEIPVLSVIDLGVVRGVAWEGETCVVTITPTYSGC
                     PAMREITQDIERTLHDHGVPQVRVRTQLAPAWTTDWMSERGRRALADYGIAPPAQQAI
                     DISGILRRADLPAVACPHCGSLDTRLVSGFGSTSCKALYRCSACREPFDYFKTH"
     gene            12444..13529
                     /gene="paaE"
                     /locus_tag="VGC69_16035"
     CDS             12444..13529
                     /gene="paaE"
                     /locus_tag="VGC69_16035"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEY0296838.1"
                     /translation="MSSSFHTLKVASVARNTRDAVVVTFDLPEALAPEFAFRPGQYLT
                     LRAQLGGEEVRRSYSICSAPGDGVLRVAIKKVDEGAFSTWANHELQPGQDLDVMPPAG
                     NFTIDFDPAQARHYAAFAVGSGITPVLSLVKTALSIEPGSRFTLFFGNRASSSVLFRE
                     EIEDLKNRYMDRFSLVYIMSRETQDIELFNGRLDGDKVAQLLRVWMPAGDIDYAFVCG
                     PHTMIESVVERLQALGVPKEHIKFELFGAPKGPRSLRTGREASVAPGKDQCEVTIVKD
                     GYSRSFAIDKNHDSVLDSALAQGIDLPFSCKGGVCSTCRCKVVEGEVDMDANFALEDY
                     EVARGFVLSCQSYPVSDKLVLDFDQET"
     gene            13649..15313
                     /gene="paaN"
                     /locus_tag="VGC69_16040"
     CDS             13649..15313
                     /gene="paaN"
                     /locus_tag="VGC69_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEY0296839.1"
                     /translation="MSTDFFERHQAVLEQAQRAAAERGYWSPHAESPSPRHYGEGAAE
                     AAASAFEALRGKPFPIDLPLAEGSAGGEVSPFGFALGITYPRVKPDALIAAAAKAQAA
                     WRRAGPRAWVGVALEILHRLNARSFEIANAVQHTTGQGFMMAFQAGGPHAQDRGLEAV
                     AYAWQEMSRIPAKSLWEKPQGKNEPIRMEKRFTVTPRGVALVIGCSTFPTWNGYPGLF
                     ASLATGNTVIVKPHPGAILPLAITVQIAREVLAEAGFDPDVVQLAAHEASDDTAQDLA
                     LRPEVKIIDFTGSTHNGNWLETNARQALVYTEKAGVNQVIIDSVDDFKGVARNLAFSL
                     ALYSGQMCTAPQNIYVPRGGIRTAAGTMSFDEVAAALGAALDKLSADPAKSVELTGAI
                     QNEATAARIDKARGLGLPVVADSRTLEHPQFPQARVRTPLLLRAEAGNEALAQEWFGP
                     IAFVVATDSTEHSIALARESVKQHGALSLSAYTTDDKVAARIQEAAEEAGVALSLNLT
                     GAVFVNQTAAFSDFHGTGANPAANAALSDSAFVANRFRVVQTRWHV"
     gene            15321..16103
                     /gene="paaG"
                     /locus_tag="VGC69_16045"
     CDS             15321..16103
                     /gene="paaG"
                     /locus_tag="VGC69_16045"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEY0296840.1"
                     /translation="MAYQNILFDLSGGIARLTLNRPDKLNSFTAAMHGEVADALGRVE
                     AEGARVLVITGAGRGFCAGQDLSERKPSNAPIDLGETVGTWYNPLVKRLHALPMPVVV
                     GVNGVAAGAGANLAFAGDIVIAKESASFIESFSKLGLIPDTGGTYALPRLVGRARALG
                     LALLGDKLPARLAQEWGLIWQCVPDDQFDAELDRLARHFAQAPTKGLAYTKRALQASL
                     GNDLAAQLDLERDLMRELGLSKDYAEGVAAFLEKRAPQFKGE"
     gene            16103..16579
                     /gene="paaI"
                     /locus_tag="VGC69_16050"
     CDS             16103..16579
                     /gene="paaI"
                     /locus_tag="VGC69_16050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEY0296841.1"
                     /translation="MDSSTPNDRVPTDPQALAQAVADGMYAKDPATQALGMRIVAVAP
                     GYAKLSMTVRGDMLNGHRTCHGGFIFTLADSTFAFSCNSRNVATVASGCTIDYLAPAY
                     EGDVLAAEGQERSLAGRTGVYDITVTNQEGRTIAIFRGRSYRIKGQVIGQAQGDMQ"
     gene            16576..>17412
                     /locus_tag="VGC69_16055"
     CDS             16576..>17412
                     /locus_tag="VGC69_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0296842.1"
                     /translation="MNTISKPGLDPIEHASRDEIETLQLQRLKWTLAHAYANVPHYKK
                     AFDAAGVHPDDLKQLSDIAKFPFTAKKDLRDNYPFGMFAVPREQVSRVHASSGTTGKP
                     TVVGYTKQDLDNWANLVARSIRAAGGKPGDIVHVAYGYGLFTGGLGAHYGAERLGCTV
                     IPMSGGQTEKQVQLIRDFGPNIIMVTPSYFCNILEEQRRQGIDPRETSLRIGIFGAEP
                     WTGQMRKDIETEAGIDAVDIYGLSEVMGPGVASECVETKDGPVIWEDHFYAEIINPAT
                     GEP"
BASE COUNT         2949 a         6021 c         5813 g         2629 t
ORIGIN      
        1 tgacgcgcgg cctgctcgaa ctcatggccg aaaccggcca cccgctcacc ctggtcacca
       61 agaacgcctt ggtcgaacgc gacatcgatc tgctttccga cctggccagg cgcaacctgg
      121 tcgtggtcta cgtcagcatc accacgctcg acgccgacat ggcccgcacg ctcgagcccc
      181 gcgcctcggc cccctggcgc cgcctgcagg ccgtgcgcac cctggccgcc gccggcgtgc
      241 cggtgggggt gctggccgcc cccatgatcc ccttcatcaa cgacgccttc ctcgagtcca
      301 tcctggccga ggcggccagt gcgggcgccc gctgggccgg ctacaccgtg gtgcgcctgc
      361 cctgggaagt cctcgcggtc ttcgagcaat ggctgtctac gcacttcccc gatcgggccg
      421 agcgcgtcct gcatcgcatc gaagacctgc gcggcggccg tcgcaacgac ccgcgcttcg
      481 gcacccgcat gcgtggcacc ggcctgtggg ccgacctgct gcgccagcgt ttttccgtgg
      541 cggtgcgcaa gcaggggctc aatctcaccc gcctgcaact cgactgccag cagttcgtcg
      601 cgccggcgcc ggacttgtcc gccggccgcg ccgccgaacg gcccgtgtcc tttcaccgtg
      661 gcgccgacga cgcccgcggc agtagcccta tccgtacccg cagcacccgc atcacctcga
      721 cgtcatccgt ttcccaactg tccttgttcg attgattcga tccgcggccg ggcagggcat
      781 ggcgccctga tccggctgct tcccgcaaag gagtgccgcc atgagcatcg ccgcttccgc
      841 tgtcctgtct tccttgccgc tttcgggttt tggcaagccc gagtccggcc aggcccagcc
      901 tggcaagtcc cgcaagcccg gcgcttcgtc gcccccgcgc cacgagccgg ccgccaaggc
      961 gccatcacgc aagtggccgt ttccgccggc cccccagccc gccgcctcgc tgggcgacgc
     1021 gcgcaagctg gcgccgaacc agatcgtctc gcggcgcgag atgctgctcg acgtgctgct
     1081 ggtcgccgcc tggggcgcca tcatccccgc gctgatgtgg ctgggggcgg cggctggttt
     1141 ctaaataggg ttttttgttg ggccagggta tccggcagaa aatagatgct atataattaa
     1201 ttggtaaatc atctattttc tatcttccat gtccaccgct cctgttgcgc aagaggctgc
     1261 ccgagcccgc ttcggctcgc tcttgtccca ggctgcccgc tcctggcgcc gcgcggcaaa
     1321 ccgccgcttg cagccttacg gcctcaccga ggccacctgg ctgccgctca ttcgcctggt
     1381 gcgttcgccc gagcccatgc gccagaaaga cctggccgct tcgctggggc tggacggctc
     1441 ctccgtggtc cgcatcctcg atgcgctgga atccgccggc ctggtcgaac gccgcgccga
     1501 ggccggtgat cgccgtgcca aggccatcct gctgacagag caggggcagg ccgtcgtggc
     1561 gcaggtcgaa cgcgtctccc aggaggtgcg cgacgaagcc ctgcaaggca tgtccgacag
     1621 cgaggtgctg caagcccatg ccttgctgga gcgcatctgc gagcggctcg acacgggaga
     1681 cgaggccgtt tccccatgag ctcaggcgtc gatgccactt cctccccctt gactgttggc
     1741 gtgcctggca gcgcaacccg ggtcgttccc aatgtgccgc tctggctgcc ggccctgttc
     1801 accttcagcg gcacgctggc catgcacatc ttcgtgcccg cgctggccat ggcggcccgc
     1861 gacctgaacg ccagcagcgc ggccatgcaa atgacggtca gcctctatgt gctgggcctg
     1921 gccggcgggc aattgatcta cggcccgctc tcggacaggt acggccggcg gccggtgctg
     1981 atggtgggcc tggtggtcta cacggtcgcg ggcctggtct gcgccttggc cgcgcaggcg
     2041 cacatcctgg tggtggcgcg gctgtttcag gccctgggcg gctgctcggg cctggtgctg
     2101 gggcgagcca tcgtgcgcga caccgcttcc accgacaatg ccgccaagcg cctggccctg
     2161 atgaacctga tggtcgtgct cgcgccggcc ctggcgccct tgataggcag cctggtggcc
     2221 gccgccgtgg gctggcgcgc cttgctcgcc ttgctgtgcc tgctgggcgt ggtcaatttc
     2281 gtgctggcct ggcgcaagct gcccgagacg catccaggca cctcgtcgct gagcctgcac
     2341 aatctcaagc acgaataccg cggcctgctc ggctcgcgcc agtttctcgg ctacaccgtg
     2401 ggcgggggct gcgccaccac gtcgatgtac gccttcattg ccagcgcgcc ctttatcttc
     2461 gtcgagcaaa tgcatcggcc cgaccacgaa gtcggcctgt acctgaccat catggtcctg
     2521 ggggcctggc tcggcaacct gctgtgcggc aggctcctgg gccgcatccc catcggccgg
     2581 ctgctgctgc tgggcaatac cctgagcctg gtctgcatcg tgctcgcgct ggcctgggcg
     2641 ctgaccggcc tgatgaccgt cttcgtcctg ctgttcctga tgttcctcta caccgtgggg
     2701 gcgggggtga gttcgccggc cgcgatgacc ctggccttga gcgtgaaccc caaggtggtc
     2761 ggctccgcat cgggcgtgta cggctgcgtg cagatggcgg tcggggcgct gtgcaccgcg
     2821 ctggccggcc tgggcagctc ccccttgttg tcggccatcg tggtcatgct ggtggcctgt
     2881 gccgtggcgc aattggcgat gcgcatggcc ctggggggag tgggggtgcg caaggcgctc
     2941 tagccctgtg ctggaagcgg ctaagtgctt tacaggcttg ggttttttgg tatagaatcg
     3001 cggattcacc ttgtcctctg cctatggaca aaacaacttt ctcctccgtg ggcaagacat
     3061 acaggagcta tccgtgaatc tcatcgctat cctcgagcag gaagaaatcg cccgcctgac
     3121 tggcggcaag gccatgcctg acttcggccc cggtgatacc gtggtcgtca acgtgaacgt
     3181 ggtcgaaggc acccgcaagc gcgtgcaggc tttcgaaggc gtggtcatcg cccgccgcaa
     3241 ccgtggcctg aattcggcct tcatcgttcg caagatctcg tccggcgaag ctgtcgagcg
     3301 tactttccag ctgtactcgc cccagatcgc ctcgatcgaa atcaagcgcc gcggcgacgt
     3361 gcgccgcgcc aagctgtact acctgcgcaa ccgctcgggc aagtcggccc gcatcaagga
     3421 aaagctggtc accaagaccc agcgccaagc gcctcaggct taaatgcgcc aagcttgatg
     3481 cgaagcagca aaaggcaccc acgggtgcct tttgctttta tatcgtccca agtaactcct
     3541 tcttgcggta actcctggtg agactttgca gaccttgttt ccgggggttg cgaagcttct
     3601 tctttgctgg aacctgtcgt gccgattggt gtcattattc tgctacgaca tgctttatta
     3661 aagcccactt tcgctgtctc cccatgcccg actctgctcc atcacgcccc agcaacccgg
     3721 ccacagccgg attcgatccg atgagccagc cctggcaggc tgcagaccag gggctgtccg
     3781 ccgttgggcg gcagtggttc gagcccgagt cctttcgccg cttgtgggca ggcatcacgg
     3841 actggcagca ggaagacagg cccggcaccg aactgcgtcc caatgggcgc gaggcggccg
     3901 tgctggtgcc cctggtcatg cgtcccgaag gcctgcgcgt catgctcacc cagcgcacgg
     3961 cgcatctgca cgatcatgcc gggcagatca gttttccggg cggccgtatc gaggccgagg
     4021 atgccagccc cgtcgatgcc gccttgcgcg aggcgctcga agaaaccggg ctgccgcaag
     4081 cgcatgtcga ggtgctgggc accatgccca agtacgccac gtccacgggg tttgccgtga
     4141 cgccggtggt gggcctggtg cgtcccgact tcacgctggc gcccgatgcc ttcgaggtgg
     4201 ccgaggtgtt cgagacgccc atggagtttc tgatgaatcc ggccaatcac cgcctgtacc
     4261 gggccacgct gcccgacggc ctccagcgcc agtatttcgc catgccctgg gaaggccgct
     4321 tcatctgggg cgccaccgcc ggcatgctgc gcaatctgta ccggaatctc tataacgccg
     4381 tgctcgccgc ggcgcatcaa tagcgcggcg gcgcttcgtg ttcgtccagg atgcgcaggt
     4441 atagcgcata gcgctccggc gcgatcaggc cggcctgcac ggcggccact acgccgcagc
     4501 cgggctcgtg ccggtggctg cagttgtaga agcggcagcg ctcgatctgc ggcaggaact
     4561 ccggaaagcc gcgcacgatg tcgtcccgcg acagatgctg caggccgaag gcctggaaac
     4621 ccggcgagtc gatcaagtcg ccgccgggtt tgtcatccag ggccggcagg tggtagatct
     4681 gcgtgctggt ggtggtgtgg cggcccatgt ccagcgcggc cgaatactcg cgcgtggcgg
     4741 cgtgggcctc gggcgccagg gtattgagca gggtggactt gcccatgccg ctctggccca
     4801 gcagcaggct ggtcttgccg gccaggcggg ccgccaactg cttgcgcacc atgtccgcat
     4861 cgtgcgcgat caattcgatg acgggcacgc ccagggccga gagcgtcgcc aggcgccggc
     4921 gcgcggcggg cagcccgtcc tgcaggtcga tcttgttgag cacgatgagc gcatcgatgc
     4981 cgacgctcca ggcgccggcc agcgcgcggc cggtcaggtc gtcggaaaac gtgggttcca
     5041 ccgccaccac gatgagcaac tggtcgacgt tggcggcaaa ttgcttggtg cgcatgtcgt
     5101 cggagcggta gagcaggttg cgccgcggca gcacggcatc gatggcgcct tcgtcgcgcc
     5161 cctgcggcga gatccgcacg aaatccccca cggccgcgcc ggctttcttg ccccgtggaa
     5221 agcattgccg cgtggtgccg tcgggaaatt ccaccatgta gtggcggccg tgcgccgcga
     5281 tgatgcgtcc ttcgagcgaa gcggtcgtct cgctcatgcg gggtggggcg aggcttgcat
     5341 gagatggcgg atgcgcacgg cggcgggcgg atggctgtcg tagaaagccg agtgtaccgg
     5401 gtcgggggtc agcgtagcgg cattgtcgtc atacagcttg accagcgccg acaccaattg
     5461 ccccgccgag ctttgctggg cggcgtagcg gtcggcttcg aactcgttgc tgcgcgaata
     5521 ccagctgccc agcggcgtga gcaggaaggt gaacaccggc agcgccagga agaacagcag
     5581 cagggccagg gcattgtcgc cgccgtcgcc gcgcgcttgc acgcccagcc cggcatagaa
     5641 ccagggctgt tgggccagcc agcccagcag ggcaaagaag gccagcgcca gcacgaagct
     5701 cagcgcgatg cgcttgacga tgtgcccgcg cgcgaaatgc cccagctcgt gggccagcac
     5761 ggcctcgatc tcgtcgccgt tcaggcgggc gagcagggtg tcgaagaaca cgatgcggcg
     5821 cgccttgccg aagccggtga aatacgcgtt gccatgcgcc gagcggcgcg agccgtccat
     5881 gacgaacagg ccgttgagcg cgaagccgca gcgctgcgcc agctgccgga tgcggctggc
     5941 cagggcgtcg tcatcgagcg gggtgaactt gttgaacagc ggcgcgatga acgtcgggta
     6001 gatgaagagc agcagcaggt tgaaagccac ccacaggccc caggcccaca gccaccaggc
     6061 ctggccggcc tcggccatca gccacagcac cgccgcggcc aaaggcaggc ccagcgccag
     6121 cgccagcacg atgcccttga ccgcgtcggc cacgaacagg gcgggggtca tgcggttgaa
     6181 gccgaagcgc gcctccaggc ggaactggcg ccacagcgtg aagggcaggc ccagcacgcc
     6241 catcaccgcc atcacggcca ccagcagcag caactgccgc gccagggcat ggccggtgag
     6301 atggccgacg gcctggtcca gccattgcaa gccgcccagc agcgtcagcc ccaccagaat
     6361 gacggcgtca tacacccgct cgaccatgcc cagccggacc cgcgccaggg tgtagctggc
     6421 ggcgcgctgg tggctggaca ggctgatgcg ggccgcgaac tcggcgggca cctggtcgcg
     6481 gtggctggcc acgtggcgca tctggcgcgc ggacagccac agccgcacgg ccatgtcggc
     6541 cagcagcaaa acgacgaaaa ccagggcaaa gcgggtcggg gaggacacag tggcgtatga
     6601 gaaaatgaat gtttgatctg gcaaattata gggcggcgga tatggcgaac gatgcaagca
     6661 atcaggtaaa gcccggcgtg gcgcaacacg agagccgtct ggtctggctc gatatggaaa
     6721 tgaccgggct cgatcccgag aaagagcgca tcatcgaagt cgccgtggtg gtcaccgagc
     6781 ccgacctcac cgtggtggcc gaaggcccgg tgctggtgat ccaccagccc gacagcctgc
     6841 tcgacgccat ggacaactgg aacaagtcca cccacggcaa gagcggcctg atcgacaagg
     6901 tcaaggcctc ctcgctgacc gaggcccagg ccgagcagca gctcatcgcg ttcctggcgc
     6961 aatacgtgcc ggcgggcaag tcgccgctgt gcggcaacac catcagccag gaccgccgct
     7021 tcatgttccg gtacatgccc gggctcgagc gcttcttcca ctaccgcaac ctggacgtca
     7081 gcacgctcaa ggaactggcc cgccgctggg cgccggccat gctcaagggc ttcgagaaga
     7141 aaagccgcca cgaagccctg gccgacatct acgagtcgat cgacgagctc aagtactacc
     7201 gcgaacactt cctgaaggtg tagcgccggc ctgcgcggcg ccttgcatgc gctgcgcggc
     7261 cagttcatcg gccagcgcgc ggaaatcctg cctttccagc agccacgata caacgaaacc
     7321 cgccaccagg ccccagaagg cggcgccgat attggcgatg cccaggtcgg ccaccgtcac
     7381 caggaaggcc accagcgcgc ccagcgaaaa gcgcgtgcgg aaggccgtgg tgaaggcgcc
     7441 ctgcagcacc cgcagcatgg ccaggccggc cagggccatg atgaaggcga tgggcgtgtg
     7501 cagcagcagc cgggtaaata ccggcgacag cacgccgaag gcgatcgcca gcaccgcgca
     7561 gcaaatgccg ccggcatagt ggctgccgcg ccggccggac gaggtgatga tggcgttgct
     7621 cgggccggcc aggcaggtgc aggcgcagcc caccgcggcg ctcagcaggg tgccgatgcc
     7681 gcaggccacc gatacggcgt tgatgggcgg ttcgtgcccg gcggccttca gcgccgcgat
     7741 gccctggccg ttctgcacga tcagtgaggt gacgaccagc ggcaccagca attcgaccat
     7801 cgccggccag gaccagaccg gcgtttcaaa gaccgggcgg acgaacacgg cgtgcgagcc
     7861 gcccaggctc atgccgccgc cctggccgga cagatagatg gccagcgcgc cgacgatgag
     7921 cgcgcccagg atgggcggca cgcggcgtcc ccacgccggc acggcgcaca gcagcagcca
     7981 ggtggcgacc atggggccgg cgatcgccag gttgtcgtgc agcgatcgca ccaggtccag
     8041 gccgaagcgc aggaatacgc cggccaccat gcccatgacg atgggcatgg gcacggcgct
     8101 catcacccgc cgcactccgc cggacaggcc cagcaccagc atcagcagcc cgctggcata
     8161 gcaggcgccc atgacttcgg gaaaggtgag atggcccagc gccggcccga tgagcaccgt
     8221 gcccggaatc gtccagaagc agaccagcgg ctgccggtag cgccagcaca tcaggatgct
     8281 gagcaggccg ttcagggcga aggcgccgaa cagccaggag gccatttgcg cttcgctgag
     8341 cccgccgtgc gtgccgacgg acagcacgat ggcaaccgga ccggttgcgc tgaagatgag
     8401 gccgatcagt ccgttggcgg cgacggtggg gttgaggtcg gcgagaaact ggcgccagcc
     8461 gggcggcgcg gaaagagggc gttcaagcat gcccgattgt agccaggggc cggcgcggcg
     8521 cgccatgccg gcccgcttca ggcgcgggtc agccgccgcg tccgccgaag cgcgccagcg
     8581 cctcgtcctg cagcggccgg tagctcggat tgtcttgccc ggcgatggcg tccaggtgga
     8641 cttccgaggc gtcgaacagc cgccagtaca gccggccgca aagcgcgcgc gcttcgtggc
     8701 cgggccagga cgcgggcagc agggcggtgg gcaatcgggg gtcgtgcagc acggcgcgcc
     8761 gccagtggtg cagcagcagc acccgggccg cgaaggccgc ggcgggcgtg gcggcctcgt
     8821 cttccagcag cttttccagc ggcgcgaagt tgcgcgcgaa cagccggtac agcccggcca
     8881 cttcgtccag gttccagcat tgcacggcca ggctggcgat gggcaggctg cccgcgccgg
     8941 ccaggtcgcg cgccgacagc accagggcct tgtcgggaat gccgagcctt tcgagtatct
     9001 cgtgcgccgc gccggcctcg gtctgggggt gggcgaagag ccccggggcc accatgccga
     9061 agccttccca cagcagttcg cggcgcagct cggcgcgttc ggccagcgcg ttgttgccga
     9121 tgcggggcag ggccaccagc gtccaggtgc cgtcccagcc gggctggacg tgctcgtaga
     9181 tgcgcagcga ggcgtgcgcg gtgtcgcgcc gcccctgctc ggagagcaga tacaggctgc
     9241 gccggccgtg gcgctcggtc tgcagccacc cctgcgccac caggcggaac acgctggtgc
     9301 gcagctgccg ctcgttgatg ccgaccggcc ccagcagttc gatcagtccg cccagccaga
     9361 tggcgccgcc gtggggcacg acggcgtcgc ccagcaggct gacgcacagc gatttggcgc
     9421 gcggcgggtc ggctttgagc aggcggccca ggaagcggtc cagcggggaa ggtgcaatag
     9481 ccatggtgcc tggcgggggg ccggcaaagg ggacagtccc gacatgatac ataaatctga
     9541 tgtgatacgg tttttcaatt gacaatcgta tttgtgtatt aaataatcta accgtgaacc
     9601 aggcctgcga ggccggcagc agccggcgcg ccggaagccg ggtgcaagga gacacatcat
     9661 gtatgcgcaa ttggtcgaaa ccggcgtggc ccgcgtccgc ggcgtcgatg aactgtccgg
     9721 ccccgagcag gccttccagc ggcgcctcga agacaacgtg cgcatcgagc cgaaagactg
     9781 gatgcccgag gcctatcgca agacgctggt ccgccagatc tcccagcacg cgcattccga
     9841 gatcgtcggc atgctgcccg agggcaactg gatcacccgc gcgcccagcc tcaagcgcaa
     9901 ggccatcctc atggccaagg tgcaggacga gggcggccac ggcctgtacc tctacagcgc
     9961 cgccgaaacc ctgggcgtct cgcgcgacgc gctcatcgac gacctgcacg cgggccgcgc
    10021 caagtactcc agcatcttca actaccccac gctcacctgg gccgacgtgg gcatggtcgg
    10081 ctggctggtc gacggctcgg ccatcatcaa ccagatcccg ctgtgccgct gctcctacgg
    10141 cccctatgcg cgggcgatgg tgcgcgtctg caaggaagag tccttccacc agcgccaggg
    10201 ctacgacctg ctggtgcaga tgtgcctgca cggcacgccc gaacaaaagg ccatgtgcca
    10261 ggacgcgctc aatcgctggt ggtggccgtc gctgatgatg ttcggcccgc ccgacggcga
    10321 ttcgcccaac agcgcgcagt ccatgctgtg gaagatcaag ctcttctcca acgacgagct
    10381 gcgccagaag atggtcgacc agaccgtgcc gcaggccgaa tacctgggcc tgacggtgcc
    10441 cgaccccgac gtgaagtgga acgccgagcg cggccactac gacttcggcg agatcgactg
    10501 gagcgagttc cacgcggtgc tcaagggcaa cggcccctgc aaccgcgagc gcctggccgc
    10561 gcgcgtcaag gcgcacgaag cgggcgcctg ggtgcgcgat gcgctggtgg cccacgccga
    10621 gaaaaaagaa cgccgcaaag ccgcatgacg cgaatgcctt ggccaagatg agcttttacg
    10681 agctcgagac gccttgagcc gcggaccccg gaggaatccg aagcacaggc gctaccagcg
    10741 ccgagcatcg gaattccgca gggcagccgc ggcggctcaa gaacgcaaag cagcgcggcc
    10801 caagaactcg aacccggcac ggctaaagaa tccaccggat agaacgctag gaaatacacc
    10861 atgagcaaag aatggcccct ctgggaagtc ttcatccgca gccagcacgg cctggcgcac
    10921 aagcacgtgg gcagcctgca cgcgcccgac gcagaaatgg ccatcaacaa cgcgcgcgac
    10981 gtctacacgc gccgcaatga aggcctgagc atctgggtgg tgcgcgccgc cgacatctcc
    11041 gccagcagcc cgtccgacaa ggaaccgctg ttcgagccgg ccaacgacaa ggtctaccgg
    11101 catccgacct tcttcccgat gcccgacgac gttcacatgt agggcagggc ggaggagaca
    11161 tggacaagac gctattccaa tacctgctgc gcctgggcga cggcgacctg atcctgtcgc
    11221 agcgcctggg cgcgctgatc gggcacggcc ccgtcctgga agaggacatg gccctggcca
    11281 acaccgcgct cgacctgctg ggccaggcgc ggttgtggct gaccctggcg ggcgaggtcg
    11341 aaggggcggg gcgcgacgaa gacgcgctgg cctaccaccg cgacgcgccg cagttcctca
    11401 acccgttgct ggtcgagcgc cccaacggcg actacgccca gacgatgacg cgccagtatt
    11461 tctacgacgt ctggcactac tacctgctgc aagggctggc gcagtcggcc gacgcgcggg
    11521 tggcggccat cgcggccaag ggcatcaagg aagtggccta ccacgtgcgc cgctcgtccg
    11581 acctggtggt gcgcctgggc gacggcaccg acgaaagcca cgcgcgcatg caggcggcgg
    11641 tggacgagtt gtggcgctac accggcgagc ttttcaccga cgacgagatc gaccgcgacg
    11701 tcgccgcgcg cggcatcggc tgcgcgatgg cgccgttgcg cgagccgtgg ctggcgcagg
    11761 tgcgcgaggt gctggccgag gccacgctgg ccgtgcccga cgaagcgcag gcctggcatg
    11821 cgctgctgag cggcggccgc cagggccgcc acaccgaaga cctgggctac gtgctggccg
    11881 agatgcagca cctgcaacgc acctatcccg gagccacgtg gtgaacgccg tatcggccgc
    11941 gtcccccgga caggtcatgc aatggctgca agccgtgccc gaccccgaga tccccgtgct
    12001 gtcggtgatc gacctgggcg ttgtgcgcgg cgttgcctgg gaaggcgaga cctgcgtggt
    12061 caccatcacg cccacgtact cgggctgccc cgccatgcgc gagatcacgc aggacatcga
    12121 gcgcacgctg catgaccacg gcgtgccgca ggtgcgggtg cggacccagc tggcgcccgc
    12181 ctggaccacc gactggatga gcgagcgcgg ccgccgggcg ctggccgact acggcatcgc
    12241 cccgccggcg cagcaggcca tcgatatctc cggcatcctg cgccgcgccg acctgccggc
    12301 cgtggcctgc ccgcattgcg gctcgctcga cacgcggctg gtgagcggct tcggctcgac
    12361 ttcctgcaag gcgctgtacc gctgcagcgc ctgccgcgaa cccttcgact atttcaagac
    12421 gcattgaccc cggggtcgac acgatgagca gttccttcca caccctgaaa gtcgcttccg
    12481 tggcgcgcaa cacgcgcgac gcggtggtgg tcaccttcga cctgcccgag gccctggcgc
    12541 ccgagttcgc cttccgcccc ggccagtacc tgacgctgcg cgcgcagctg ggcggggaag
    12601 aggtgcgccg ttcgtattcc atctgctcgg cgcccggcga cggcgtgctg cgcgtggcca
    12661 tcaagaaggt cgacgagggc gcgttctcga cctgggccaa ccacgagctg cagccggggc
    12721 aggacctcga cgtgatgccg ccggccggca atttcaccat cgacttcgac cccgcccagg
    12781 cgcgccatta cgcggccttc gcggtgggca gcggcatcac gccggtgctg tcgctggtga
    12841 agacggcgct gtccatcgag ccgggcagcc gcttcacgct gttcttcggc aaccgggcct
    12901 cgtcgtcggt gctgttccgc gaagagatcg aagacctcaa gaaccgctac atggaccgct
    12961 tctcgctggt ctacatcatg agccgcgaga cgcaggacat cgagctcttc aacggccggc
    13021 tcgacggcga caaggtggcc cagctgctgc gcgtctggat gccggccggc gacatcgact
    13081 acgccttcgt ctgcggcccg cacaccatga tcgaaagcgt ggtcgagcgc ctgcaggcgc
    13141 tgggcgtgcc caaggagcac atcaagttcg agctgttcgg cgcgcccaag ggccccaggt
    13201 cgctgcgcac cggccgcgag gcttccgtcg cgcccggcaa ggaccagtgc gaggtcacca
    13261 tcgtcaagga cggctacagc cgcagcttcg ccatcgacaa gaaccacgac agcgtgctgg
    13321 attcggcgct ggcgcagggc atcgacctgc cgttctcgtg caaggggggc gtgtgctcca
    13381 cctgccgctg caaggtcgtc gaaggcgagg tcgacatgga cgccaatttc gcgctcgaag
    13441 actacgaagt ggcgcgcggc ttcgtgctga gctgccagag ctatccggtc agcgacaagc
    13501 tggtgctgga cttcgaccag gaaacctgaa gaaaacgcgc gcggccccgg ttacggggcc
    13561 gcgcgccatg aagaacgcaa acccgcacgt cagaaaacag gcattacata ggtaagtctc
    13621 ggcttatctc aatcactgga gagacatcgt gtccaccgac ttcttcgaac gccaccaggc
    13681 cgtgctggag caggcccagc gcgccgccgc cgagcgcggc tactggagcc cccacgccga
    13741 atcgcccagc ccgcgccatt acggcgaagg cgccgccgag gcggcggcct ccgccttcga
    13801 ggcgctgcgc ggcaagccgt tccccatcga cctgccgctg gccgagggca gtgcgggggg
    13861 cgaggtctcg cccttcggct tcgcgctggg catcacctac ccgcgcgtca agcccgacgc
    13921 gctgatcgcc gcggcggcca aggcgcaggc tgcatggcgc cgcgccggcc cgcgcgcctg
    13981 ggtgggcgtg gcgctggaga tcctgcaccg gctgaacgcg cgcagcttcg agatcgccaa
    14041 tgccgtgcag cacaccaccg gccagggctt catgatggcc ttccaggccg gcggcccgca
    14101 cgcccaggac cgcggcctgg aagccgtggc ctatgcctgg caggaaatgt cgcgcatccc
    14161 ggccaagtcg ctgtgggaaa agccgcaggg caagaacgag cccatccgca tggaaaagcg
    14221 cttcaccgtg accccgcgcg gcgtggccct ggtgatcggc tgttccacgt tccccacctg
    14281 gaacggctat cccggcctgt tcgccagcct ggccacgggc aatacggtga tcgtcaagcc
    14341 gcaccccggc gccatcctgc ccctggcgat cacggtgcag atcgcccgcg aggtgctggc
    14401 cgaggccggg ttcgacccgg acgtcgtgca actggccgcc cacgaagcca gcgacgacac
    14461 cgcccaggac ctggcgctgc gccccgaggt caagatcatc gacttcaccg gcagcacgca
    14521 caacggcaat tggctggaaa ccaacgcccg ccaggccctg gtctataccg agaaggccgg
    14581 cgtcaaccag gtcatcatcg actcggtcga cgacttcaag ggcgtggcgc gcaacctggc
    14641 gttctcgctg gccctgtact cgggccagat gtgcacggca ccgcagaaca tctacgtgcc
    14701 gcgcggcggc atccgcacgg ccgcgggcac gatgagcttc gacgaagtgg ccgccgccct
    14761 gggcgcggcg ctcgacaagc tctcggccga cccggccaag tcggtcgagc tgaccggcgc
    14821 catccagaac gaggccaccg cggcccgcat cgacaaggcg cgcggcctgg gtctgccggt
    14881 cgtggccgac agccgtacgt tggaacaccc gcagtttccg caggcccgcg tgcgcacgcc
    14941 gctgcttttg cgcgccgaag cgggcaacga ggcgctggcc caggaatggt tcggccccat
    15001 cgccttcgtc gtggctaccg actccaccga acacagcatc gcgctggcgc gcgagagcgt
    15061 gaagcagcac ggtgcgctga gcctgtcggc ctacaccacc gacgacaagg tcgcggcccg
    15121 gatacaggaa gccgccgagg aagccggcgt ggcgctctcg ctcaacctga ccggcgccgt
    15181 gttcgtcaac cagacggcgg ccttcagcga tttccacggc accggcgcca acccggccgc
    15241 caacgcggcg ctgtcggact cggctttcgt cgccaatcgt ttccgcgtcg tgcagacgcg
    15301 ctggcacgtg tgaggcaagc atggcctatc aaaatattct tttcgatctg agcggcggca
    15361 tcgcgcggct caccctcaac cgtccggaca agctcaacag cttcacggcg gcgatgcacg
    15421 gcgaggtggc cgacgcgctc ggccgcgtcg aagccgaggg cgcccgcgtg ctggtgatca
    15481 ccggcgcggg ccgcggcttc tgcgccgggc aggacctgag cgagcgcaag ccgtccaatg
    15541 cgcccatcga cctgggcgag accgtcggca cctggtacaa cccgctggtc aagcgcctgc
    15601 atgcgctgcc catgccggtg gtggtgggcg tcaacggcgt ggccgccggc gccggggcca
    15661 acctggcctt cgccggcgac atcgtcatcg ccaaggaatc ggccagcttc atcgagtcgt
    15721 tctccaagct ggggctgatt cccgacacgg gcggcaccta cgcgctgccg cgcctggtgg
    15781 gccgcgcccg cgccctgggc ctggccctgc tgggcgacaa gctgccggcc aggctggcgc
    15841 aggagtgggg cctgatctgg caatgcgtgc ccgacgacca gttcgacgcc gagctcgacc
    15901 ggctggcgcg gcatttcgcg caggcgccca ccaaggggct ggcctatacc aagcgggcgc
    15961 tgcaggccag cctgggcaat gacctggccg cgcagctgga tctggagcgc gacctgatgc
    16021 gcgaactggg actaagcaag gattacgccg aaggcgtggc ggctttcctc gaaaagcgcg
    16081 cgccgcaatt caagggggaa tgatggacag ttccaccccg aacgataggg ttccgaccga
    16141 tccgcaggcc ctggcgcagg ccgtggccga cggcatgtat gccaaggacc ctgccacgca
    16201 ggcgctgggc atgcgcatcg tggccgtcgc gccgggctac gccaagctgt cgatgacggt
    16261 gcgcggcgac atgctcaacg gccaccggac ctgccacggc ggcttcatct tcaccctggc
    16321 cgacagcacc ttcgcgttct cgtgcaactc gcgcaacgtg gccacggtgg cctcgggctg
    16381 caccatcgac tacctggcgc cggcctatga gggcgacgtg ctcgccgccg aagggcagga
    16441 gcggtcgctg gcgggccgca cgggcgtcta cgacatcacc gtcaccaacc aggagggccg
    16501 caccatcgcc atcttccgcg gccgctcgta ccggatcaag ggccaggtca tcggccaggc
    16561 ccaaggagac atgcaatgaa cacaataagc aagcccggcc tggaccccat cgagcacgcc
    16621 agccgcgacg agatagagac cctgcagctg cagcgcctca aatggacgct ggcccacgcc
    16681 tacgccaacg tgccgcatta caaaaaggcc ttcgacgcgg ccggcgtgca tccggacgac
    16741 ctcaagcagc tgtcggacat cgccaagttc cctttcaccg ccaagaaaga cctgcgcgac
    16801 aactacccct tcggcatgtt cgccgtgccg cgcgagcagg tgtcgcgggt gcacgcttcg
    16861 agcggcacca ccggcaagcc cacggtggtc gggtatacca agcaggacct ggacaactgg
    16921 gccaacctgg tggcgcgttc gatccgcgcg gccggcggca agcccggcga catcgtgcac
    16981 gtggcctacg gctacggcct gttcactggc ggcctgggcg cgcactacgg cgccgaacgg
    17041 ctgggctgca cggtcatccc catgtccggc gggcagaccg aaaagcaggt gcagctgatc
    17101 cgcgacttcg ggcccaacat catcatggtc acgccttcct acttctgcaa tatcctggaa
    17161 gagcagcggc gccagggcat cgacccgcgc gagacttcgc tgcgcatcgg catcttcggc
    17221 gccgagccct ggacgggcca gatgcgcaag gacatcgaaa ccgaggccgg catcgacgcg
    17281 gtcgacatct acggcctgtc cgaagtgatg ggaccgggcg tggccagcga atgcgtcgag
    17341 accaaggacg gcccggtgat ctgggaagac cacttctacg ccgagatcat caaccccgcc
    17401 accggcgagc cg
//
LOCUS       DASWPO010000306         4721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2751195, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000306 DASWPO010000000
VERSION     DASWPO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751195"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..715)
                     /gene="hisG"
                     /locus_tag="VGC69_16060"
     CDS             complement(71..715)
                     /gene="hisG"
                     /locus_tag="VGC69_16060"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0296843.1"
                     /translation="MTLALSKGRIFDDTLPLLAEAGISVPESPEDSRKLILPTSDPGL
                     RLIIVRTTDVPTYVQYGAADLGIAGKDVLIEHAEDQPGRLYQPIDLDIARCRLCVAVR
                     QGFDYEAAVHQGARLRVATKYPRAAREHFASKGVYADIIKLYGSMELAPLVGLADCIV
                     DLVSTGNTLRANDLVAVEDIMPISSRLIVNQAALKTRRARLQPIIDAFERATLN"
     gene            complement(781..2043)
                     /gene="murA"
                     /locus_tag="VGC69_16065"
     CDS             complement(781..2043)
                     /gene="murA"
                     /locus_tag="VGC69_16065"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0296844.1"
                     /translation="MDKIRITGGRALRGDVIISGAKNAALPILCAGLLTADPLHLTNV
                     PALNDIGTTLKLLRLMGVRAEQEGDAVVLQADKVDKLEAPYELVKTMRASILVLGPLL
                     ARFGEVRVSFPGGCGIGQRPVDQHIKGMAALGADIEIEHGFIVARAKRLKGASIRTDM
                     VTVTGTENLLMAAVLAEGRTVLENAAREPEVVDLAELLIKMGAHIQGHGSDRIVIEGV
                     ERLHGAEHRVIPDRIEAGTFLCAVGAAGGDITLRNVDAGQLGATLDKLAEAGLTIETG
                     PGWIRGAISGRPRAVGARTHEYPGFATDMQAQLMALDAVAEGTSVIVETIFENRYMHV
                     QELRRMGADIDIDGHTAVVRGVARLSGAAVMATDLRASAGLIIAGLVAEGETVVDRVY
                     HLDRGYDRIERKLGALGAQIERITGKSA"
     gene            complement(2045..2284)
                     /locus_tag="VGC69_16070"
     CDS             complement(2045..2284)
                     /locus_tag="VGC69_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY0296845.1"
                     /translation="MLPTPEQVRDYIVAGLPCEHIDVTGDGSHFEAVIVSAAFEGMRP
                     IARHQLVYTALGDRMKAEIHALSMRTLTPGEYAKG"
     gene            complement(2310..3125)
                     /locus_tag="VGC69_16075"
     CDS             complement(2310..3125)
                     /locus_tag="VGC69_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296846.1"
                     /translation="MNAPIASTAAPAMIRPQMAAGLGFPTLLKKELLRFWKVAFQTIA
                     APVITALLYLVVFAHVLQDRLMMYGHVPYTAFLIPGLMMMSMLQNAFSNPSSSLIQSR
                     MSGNLVFILLPPMSHYELFGAYVLAAVARALAVGLCVWLVALYFVPLAPAQPLWVLVF
                     ALLSCGIMGTLGMIAGLWSEKFDQLSAFQNFLIMPATFLSGVFYSIHTLPPFWQAVSH
                     WNPLFYTIDGFRYGFFAVADVSPWRSLAVVAGVFVVLMLAALRLLASGYKLRH"
     gene            complement(3122..3925)
                     /locus_tag="VGC69_16080"
     CDS             complement(3122..3925)
                     /locus_tag="VGC69_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296847.1"
                     /translation="MSAAVSLEHISKIYPPRSRGWRKWLGQPASPGFQALDDVSLRIE
                     PGEFFGLLGPNGAGKTSLISILAGLARATRGRASVCGYDVVADYKMARRALGVVPQEL
                     VYDPFFTVRETLRNQSGYFGLRNNDDWIDEILHNLGLSDKADSNMRALSGGMKRRVLV
                     AQALVHRPPVIVLDEPTAGVDVDLRHTLWQFISRLNREGHTIMLTTHYLEEAEELCGR
                     IAMLKRGRLVALDTTAALLARVGGADLEDAFVRIMREEMPAIETQEVAA"
     gene            4115..>4721
                     /locus_tag="VGC69_16085"
     CDS             4115..>4721
                     /locus_tag="VGC69_16085"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296848.1"
                     /translation="MSSHPLDVSRQVKPGVFYAHYDLVSSTSRQHLLEWRDICSHEID
                     LLLPSFSELAERQFNVVSDRYQVGDIVFADARAGGQVLERSLTRISRDRLEGFVFHVF
                     LEGCTHSVSVRPSGQGATPRSGCIFVRDMAQPVRMQRTDYRSLTFAVPTRVVAEVLPE
                     PDMLHGQMLSADMPGVRLLINHVAALGRSIPNMDAEVAASAM"
BASE COUNT          745 a         1477 c         1720 g          779 t
ORIGIN      
        1 ggcccttcgg gctgcccgcc gctcaaagcg ccgtggcccc tgggtccgcc ctcgcaatgc
       61 tcaagtcagt tcaattcagc gtagcacgct cgaatgcgtc gatgatgggc tgcagccggg
      121 cgcggcgggt tttcagcgcg gcctggttga cgatcaggcg cgaggagatc ggcatgatgt
      181 cttccacggc caccaggtcg ttggcgcgca gggtgttgcc ggtggagacc aggtcgacga
      241 tgcagtcggc caggccgacg agcggggcga gttccatcga gccgtacagc ttgatgatat
      301 ccgcatacac gcccttgctg gcaaaatgct cgcgggccgc gcgcggatac ttggtggcga
      361 cgcgcaggcg cgcgccctgg tgcacggcgg cttcgtagtc gaagccctgg cgcacggcca
      421 cgcacaggcg gcagcgcgcg atgtccaggt cgatgggctg gtagagccgg ccgggctggt
      481 cctcggcgtg ctcgatcagc acgtccttgc cggcgatgcc caggtcggcc gcgccgtatt
      541 gcacgtaggt gggcacgtcg gtggtgcgca cgatgatcag gcgcaggccc gggtcgctgg
      601 tgggcaggat gagcttgcgg gagtcttccg ggctttccgg cacggaaatg ccggcctcgg
      661 ccagcagcgg cagggtgtcg tcgaagatgc ggcccttgga cagggcgagc gtgatgggcg
      721 tcctcttggc gctctgcgcc gtccccccgg gggggatctc cagcgggctc gaggtcgtgc
      781 tcatgcggat tttcccgtaa tgcgctcgat ctgggcgccc agggcgccca gcttgcgttc
      841 tatgcggtca tagccgcggt ccaggtggta gacgcggtcg accacggtct cgccctcggc
      901 caccagcccg gcgatgatca ggccggccga ggcgcgcagg tcggtggcca tgaccgcggc
      961 gcccgacagg cgggccacgc cgcgcaccac ggcggtgtgg ccgtcgatgt cgatgtcggc
     1021 gcccatgcgg cgcagttcct gcacgtgcat gtagcggttc tcgaagatgg tctcgacgat
     1081 gaccgaggtg ccttcggcga cggcgtccag cgccatgagc tgggcctgca tgtcggtggc
     1141 gaagccgggg tattcgtggg tgcgggcgcc cacggcgcgc gggcggcccg agatggcgcc
     1201 gcggatccag ccggggccgg tctcgatggt cagccccgcc tcggcgagct tgtcgagcgt
     1261 ggcgcccagc tggcccgcgt cgacattgcg cagggtgatg tcgccgccgg ccgcgcccac
     1321 ggcgcacagg aaggtgccgg cttcgatgcg gtcggggatg acgcggtgtt ccgcgccgtg
     1381 caggcgctcg acgccttcga tgacgatgcg atcggaaccg tggccctgga tgtgggcgcc
     1441 catcttgatg agcagctcgg ccaggtcgac cacctcgggc tcgcgcgcgg cgttctccag
     1501 cacggtgcgg ccctcggcca ggacggcggc catgagcagg ttctcggtgc cggtgacggt
     1561 gaccatgtcg gtgcggatcg aggcgccctt caggcgcttg gcgcgggcca cgatgaagcc
     1621 gtgctcgatc tcgatgtcgg cgcccagcgc ggccatgccc ttgatgtgct ggtcgaccgg
     1681 gcgctggccg atgccgcagc cgccggggaa actcacgcgc acttcgccga agcgcgccag
     1741 cagcgggccc agcaccagga tggaggcgcg catggtcttg accagctcgt agggcgcctc
     1801 gagcttgtcg accttgtcgg cctgcaggac gacggcgtcg ccttcctgct cggcgcgcac
     1861 gcccatcagg cgcagcagct tgagcgtggt gccgatgtcg ttcaaggccg gcacattggt
     1921 caggtgcagc gggtcggccg tgagcaggcc ggcgcacagg atgggcagcg ccgcgttctt
     1981 ggcgccggag atgatgacgt cgccgcgcag ggcgcggccg ccggtgatgc ggattttgtc
     2041 catttcagcc cttggcgtat tcgccgggag tgagggtacg catcgacaat gcgtggattt
     2101 cggctttcat gcggtcgccc agcgcggtgt agacgagctg gtggcgggcg atggggcgca
     2161 tgccttcgaa ggcggcgctg acgatgacgg cctcgaagtg ggagccgtcg ccggtgacgt
     2221 cgatgtgctc gcagggcagg ccggcgacga tgtagtcgcg cacctgttcg ggagtaggaa
     2281 gcatggacga atcctgtgcg gtatcggtat tagtgacgga gcttgtagcc gctggccaag
     2341 agtcgcagcg cggccagcat gagcaccacg aagacgccgg ccaccacggc caggctgcgc
     2401 cagggcgaca cgtcggccac ggcgaaaaag ccgtagcgaa agccgtcgat cgtgtagaag
     2461 agcgggttcc agtgcgacac ggcctgccag aagggcggca gggtatggat ggagtagaac
     2521 acgccggaca ggaaggtggc cggcatgatg aggaagttct ggaaggccga gagctggtcg
     2581 aatttttccg accacaggcc ggcgatcatg cccagcgtgc ccatgatgcc gcaggacagc
     2641 agggcgaaga ccagcaccca cagcggctgc gccggcgcga gcggcacgaa gtacaaggcc
     2701 accagccaca cgcacaggcc caccgccagg gcgcgcgcca cggcggccag cacataggcg
     2761 ccgaacagct cgtagtgcga catgggcggc agcaggatga acaccaggtt gcccgacatg
     2821 cggctctgga tgagcgacga ggacgggttg gagaaggcgt tctgcagcat gctcatcatc
     2881 atcagcccgg ggatgaggaa ggccgtgtag ggcacgtggc cgtacatcat cagccggtcc
     2941 tggagcacgt gggcgaacac caccaggtag agcagggcgg tgatgacggg ggcggcgatg
     3001 gtctggaagg ccaccttcca gaagcgcagc agctccttct tcaacagcgt gggaaagccc
     3061 aggccggccg ccatctgggg ccggatcatg gcgggcgcgg cggtcgatgc gataggcgcg
     3121 ttcatgcggc gacctcctgc gtttcgatgg cgggcatctc ttcgcgcatg atgcgcacga
     3181 aggcgtcttc gaggtcggcg ccgcccacgc gcgccagcag ggcggccgtg gtgtccagcg
     3241 ccaccagccg gccgcgcttg agcatggcga tgcggccgca cagttcctcg gcttcttcga
     3301 ggtagtgggt ggtgagcatg atggtgtggc cttcgcggtt caggcgcgag atgaactgcc
     3361 acagggtgtg gcgcaggtcg acgtcgacgc cggcggtggg ttcgtcgagc acgatgaccg
     3421 gcggacggtg caccagggcc tgggccacca gcacgcgccg cttcatgccg cccgacaggg
     3481 cgcgcatatt gctgtcggct ttgtcggaca ggcccaggtt gtggaggatc tcgtcgatcc
     3541 agtcgtcgtt gttgcgcagg ccgaaatagc cggactggtt gcgcagcgtc tcgcggaccg
     3601 tgaagaaggg gtcgtagacc agctcctgcg gcaccacgcc cagggcgcgc cgggccatct
     3661 tgtagtcggc cacgacgtcg tagccgcaca cgctggcgcg gccccgggtg gcgcgggcca
     3721 ggccggccag gatggagatc agcgaggtct tgcccgcgcc gttggggccc agcaggccga
     3781 agaattcgcc cggctcgatg cgcaggctga cgtcgtccag ggcctggaag cccgggctgg
     3841 cgggctggcc gagccatttg cgccacccgc gcgagcgcgg cggatagatt ttggagatgt
     3901 gttcgagact gacggcggca gacatatgtt tttttggaaa gcccggccat tgggcgggct
     3961 gaaaaagagc gaatctccca ttatataagg gctatcccga agagcctctt acatcttcgg
     4021 tcgcaaattg gcacagatcg cctaggtagc tgaaatttag tccctacatt agcagggtta
     4081 tttggcaagc tcggtctatc cacatttcaa ctgcatgagc tcccatcccc tggatgtctc
     4141 gcgccaggtc aagcccggcg tgttctatgc gcactacgac cttgtgtcga gcacctctcg
     4201 gcagcacctg ctcgagtggc gcgacatctg cagtcacgag atcgacctgc tgctgccgtc
     4261 gttctccgag ctggccgagc ggcagttcaa cgtggtcagc gaccgctacc aggtgggcga
     4321 catcgtgttc gcggacgccc gggccggcgg ccaagtgctc gagcgttcgc tcacccgcat
     4381 ctcgcgcgac cgcctggagg gctttgtttt ccatgtgttc ctggaaggct gcacgcacag
     4441 cgtgtcggtg cggccgtcgg ggcagggcgc cacgccgcgc agcggctgca tcttcgtgcg
     4501 cgacatggcc cagccggtgc gcatgcagcg caccgactat cgctcgctga ccttcgcggt
     4561 gccgacccgc gtggtggccg aagtcctgcc cgagcccgac atgctgcacg gccagatgct
     4621 gagcgcggac atgccggggg tccgcttgct catcaaccac gtggcggcgc tgggccgcag
     4681 cattcccaat atggatgccg aggtggccgc aagcgccatg c
//
LOCUS       DASWPO010000307         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1055163, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000307 DASWPO010000000
VERSION     DASWPO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055163"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VGC69_16090"
     CDS             <1..1765
                     /locus_tag="VGC69_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0296849.1"
                     /translation="GCLVAIEVILLFAGVVWRYGFDHPLVWTDEVAEMLFLWLVSLGA
                     VVALRRAEHMRMTVVLNRLGPRTQRLAGCLAATLVVVVALGLLVPGLGYAQQQAAILT
                     PALQLPGSWETYGQLVALVLLLYVALRQLLANANWAEIASVLIFSAVVWFALDRIENA
                     FDLGNAELLIYFIGMAGICIFLGVPIAFSFAISTFAYIHFTSTIPLSVIISQMDQGMS
                     SIELLAVPMFVVLGLLLEMTGIARAMIHFLASLVGHRRGGLNYVLIAAMYLISGISGS
                     KAADQAAVAPILLPEMRRRGVPPGELAALLASSAAMSETIPPSLVLIIVGSVTGVSIA
                     ALFTGGLLPAALAAAGLVLLVFFRTRKDHPKGEQAGAREVGRLFVISLPALILPFLIR
                     YVVLAGITTATEVATVGVVYSVLVGLFVYRCFDWRRLFPILVETATLSGAILFIIGSA
                     SAMAWALTQAGFAQSLAAMMTSLPGGRAGFLAISVVLFIVLGSVLEGLPAMVLFGPLL
                     FPVAQQIGIHQVQYGIVAILAMGIGLFSPPFGVGFYQTCLISKVSSDEAFGRIWPYLA
                     VLVVALIIVACVPWLSIGFLG"
     gene            1791..2150
                     /locus_tag="VGC69_16095"
     CDS             1791..2150
                     /locus_tag="VGC69_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0296850.1"
                     /translation="MASASPFRVQFRMRVYRGGDIAIGPGKIALLEAIEQTRSISAAA
                     RKLGMSYRRAWLLVDELNRSLREPAVATAVGGAHGGGSSVTPTGLALIQHYRAIETAA
                     ESAAAGEIRALKRLLAP"
     gene            2207..2884
                     /gene="modB"
                     /locus_tag="VGC69_16100"
     CDS             2207..2884
                     /gene="modB"
                     /locus_tag="VGC69_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY0296851.1"
                     /translation="MTKLIADFDWFPLVLSLKVAALATVLALVVGVALGWLFVRRPFP
                     GSALLQTLCMLPLVLPPTVVGYGILVAAGRYSPLGQWLRDHFDYSLIFNWHGAVVASA
                     VVALPLVLESASAAFTGIDPSLEAAARTLGARPWSVFRRVTLPLAWPGILAGTLLAYA
                     RAMGEFGASLMVAGSIPHQTQTLPMAIYDAMQAGQDSQALLLVLVTSVISIGVLTVSK
                     RFFSLNH"
     gene            2901..3668
                     /gene="modA"
                     /locus_tag="VGC69_16105"
     CDS             2901..3668
                     /gene="modA"
                     /locus_tag="VGC69_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0296852.1"
                     /translation="MTTPTRPLIRLRHLGLALLLACGAAHAEDLTVSAAASLTNAYTD
                     LGKQFEAQVPGATVKLNFAASGVLLQQMSQGAPVDVFASADEATMDKAVSQKLIDTAT
                     RRDFATNSLVLITPTQGGPAIASAQDLAGQAVKRIAIGKPATVPVGSYTQQSLQAAQL
                     WTALEPKVIQADSVRQVLDYVSRGEVDAGFVYRTDAAIMPDKVKVAATVPTAQPVSYP
                     VAVLADSKHKALAGKFVDFLATPAARAVLDRYGFGKP"
     gene            3763..>4256
                     /locus_tag="VGC69_16110"
     CDS             3763..>4256
                     /locus_tag="VGC69_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0296853.1"
                     /translation="MKQVIACIDGIARSPGICDYAIWAANRLDTDLDFLHVTEQTPDY
                     LPVAELTGNVWLSTQESLLHELADLDRQRNDLLLEQGRQSLQAAVERARSQGATRIDA
                     KQRQGNLLDILLELEPDTRLFVLGHHEQPIKASRLLPPDHRLEFVVRGLHRPIMVASP
                     FFREP"
BASE COUNT          587 a         1556 c         1336 g          777 t
ORIGIN      
        1 cggttgcctc gtcgccatcg aagtcatcct gctgttcgcg ggggtagtct ggcgctatgg
       61 gttcgaccat cccctggtct ggacggacga ggtcgcggag atgctgtttc tctggctggt
      121 gagcctcggc gcggtggtcg cattgcgccg cgccgagcac atgcgcatga ccgtagtgtt
      181 gaaccgcctg gggccccgca cccaacgcct ggccggctgc ctcgcggcca cgctcgtcgt
      241 ggtcgtggcg ctgggcctgc tcgtccccgg cctgggctat gcccagcagc aggcggcgat
      301 cctgacgccg gcgttgcagc tgcccggctc ttgggaaacc tacggacagc tcgtggcgct
      361 cgtgctgctg ctttacgtgg cgctgcgcca attgctggcc aatgcgaact gggccgagat
      421 cgcgtccgtg ctgatcttca gcgccgtcgt ctggttcgcg ctcgaccgca tcgagaatgc
      481 attcgacctc ggcaacgccg aactcctgat ctatttcatc ggcatggccg gcatctgcat
      541 ctttctcggc gtgccgatcg ccttcagctt cgcgatctcg accttcgcct acatccattt
      601 caccagcacg attccgctct cggtcatcat cagccagatg gatcaaggca tgtcctccat
      661 cgagctgctg gccgtgccga tgttcgtcgt gctcggcctg ctgctggaga tgaccggcat
      721 cgcccgcgcc atgatccatt tcctcgcctc cctggtcggc caccggcgcg gcgggctgaa
      781 ctacgtgctg atcgcggcca tgtacctgat ctcgggcatc tcgggctcca aggccgccga
      841 tcaggcggcc gtcgcgccga tactgctgcc ggagatgcgc cggcgcggcg tgccgccggg
      901 cgagctcgcg gcgctgctgg cctcgtcggc cgccatgtcg gagacgattc cgcccagcct
      961 cgtgctcatc atcgtcggct ccgtcaccgg cgtttcgatc gccgcgctat ttaccggcgg
     1021 cctgctgccg gcggccctgg ccgccgccgg gctggtgctg ctcgtgttct tccgcacccg
     1081 caaggaccat cccaagggcg aacaagccgg cgcgcgcgaa gtcggccggc tgttcgtgat
     1141 atcgctgccg gcgctcatcc tgccgttcct catccgctac gtcgtgctgg cgggcatcac
     1201 gacggcgacg gaagtcgcga cggtcggcgt cgtctattcg gttctggtcg gcctcttcgt
     1261 ctatcgctgc ttcgactggc gccgcctgtt cccgatcctg gtggagacgg cgaccttgtc
     1321 gggggccatc ctgttcatca tcggctcggc gagcgccatg gcgtgggcgc tgacccaggc
     1381 cggtttcgcg cagtccttgg ccgccatgat gaccagcctg cccggcgggc gcgccggctt
     1441 tctcgccatc tcggtggtgc tgttcattgt gcttggcagc gtgctggaag ggctgccggc
     1501 catggtgctg ttcggcccgc tcctgtttcc ggtggcccag cagatcggca tccatcaggt
     1561 gcagtacggc atcgtcgcca tcctggccat gggcatcggc ctgttctcgc cgcccttcgg
     1621 cgtgggtttc taccagacct gcctgatcag caaggtgtcg agcgacgagg ccttcggccg
     1681 gatctggccc tatctggccg ttctggtcgt ggcactgatc atcgtcgcct gcgtaccttg
     1741 gctgtcgatc ggcttcctcg gttgatcgga tccggtcgcg catgcggaat atggccagcg
     1801 cctccccgtt ccgtgtgcaa ttccgcatgc gcgtctaccg cggcggcgac atcgccatcg
     1861 ggccgggcaa gatcgccctg ctcgaagcca tcgaacagac gcgctccatc agcgcggcgg
     1921 cccgcaaact gggcatgtcc taccgccgcg cctggctgct ggtcgatgaa ctcaaccgct
     1981 cgctgcgcga acccgccgtg gcgaccgccg tcggcggcgc gcacggcggc ggctcgtccg
     2041 tcacgccgac cgggctggcg ctgatccagc attaccgcgc catcgaaacc gcggccgaat
     2101 ccgccgcggc cggcgagatc cgggccctca agcgcctgct ggcgccctag cgccctccgg
     2161 gcctgtcagc aaactgcctg ccgtcttacg ctatatttca gcggatatga cgaagctgat
     2221 cgccgatttc gactggttcc ccctggtgct gtcgctgaag gtcgcggccc tggccaccgt
     2281 gctggccctg gtcgtcgggg tagccctggg ctggctgttt gtgcgccggc cctttcccgg
     2341 cagcgccttg ctgcagacgc tgtgcatgct gccgctggtg ctgccgccca cggtggtcgg
     2401 ctacggcatc ctggtggccg ccggccgcta cagcccgctg ggccagtggc tgcgcgatca
     2461 cttcgactac tcgctcatct tcaattggca cggcgccgtg gtggcctccg ccgtcgtggc
     2521 cctgcccctg gtgctggaat ccgccagcgc cgccttcacc ggcatcgacc cgtcgctgga
     2581 ggcggccgcc cgcaccctgg gcgcccgccc ctggtcggta ttccggcgcg tgaccctgcc
     2641 actggcctgg cccggcatcc tggccggcac gctgctggcc tacgcgcgcg ccatgggcga
     2701 attcggcgcc tcgctgatgg tggccgggtc cattccgcac cagacccaga cgctgcccat
     2761 ggccatctac gacgccatgc aggccggcca ggacagccag gccctgctgc tggtgctggt
     2821 gacctcggtc atttcgatag gcgtactcac cgtttccaag cgttttttct ccctgaacca
     2881 ctaaccttgc gacctctgcc atgactaccc ctacccgccc cctgatccgc ctgcgccacc
     2941 tcggcctcgc cctgctcctg gcctgcggcg ccgcccatgc cgaagacctc accgtctcgg
     3001 ccgccgccag cctcaccaat gcctataccg atctcggcaa acagttcgag gcgcaggtgc
     3061 ccggcgccac cgtgaagctg aacttcgccg cctcgggcgt gctgctgcag cagatgtcgc
     3121 agggcgcccc ggtggacgtc ttcgccagcg ccgacgaggc caccatggac aaggctgtgt
     3181 cgcaaaagct gatcgacacg gccacccgcc gcgatttcgc caccaacagc ctggtgctca
     3241 tcaccccgac ccagggcggc ccggccatcg cctcggcgca ggatctcgcc ggccaggccg
     3301 tcaagcgcat cgccatcggc aagccggcca ccgtgccggt cggcagctac acgcagcagt
     3361 cgctgcaggc cgcccagctc tggaccgcgc tcgaacccaa ggtcatccag gccgacagcg
     3421 tgcgccaggt gctcgactac gtgagccgcg gcgaagtcga cgcgggcttc gtctaccgca
     3481 ccgacgcggc catcatgccc gacaaggtca aggtcgccgc caccgtgccg accgcccagc
     3541 ccgtgtcgta tccggtggcc gtgctggccg acagcaagca caaggcgctg gccggcaagt
     3601 tcgtggactt tcttgccacg cccgcggccc gcgccgtgct ggatcgctac ggattcggca
     3661 agccgtaatc ccggacaggg acatggctgg cgcatggaca tagaatagaa gcggaagcgt
     3721 gcgcgcgcga accgcgcgca cgactcgccg cgggagacaa ccatgaaaca agtcatcgcc
     3781 tgcatcgacg gcattgcccg ctcgccgggc atctgcgact acgccatctg ggcagccaat
     3841 cggctcgaca ccgacctgga ctttctgcac gtcacggaac aaacgcccga ctacctgccc
     3901 gttgccgaac tcaccggcaa tgtctggctc agcacgcagg aatcgctgct gcacgagctg
     3961 gccgacctcg accgacagcg caacgacctg ctgctggagc agggccgcca gtccctgcag
     4021 gcggccgtgg agcgggcgcg cagccagggc gccacgcgca tcgacgccaa gcagcgccag
     4081 ggcaatctgc tcgacatcct gctcgaactc gagcccgaca cgcggctttt cgtgctgggc
     4141 caccacgagc agcccatcaa ggcttcgcgg ctgctgcccc ccgatcatcg gctggaattc
     4201 gtggtgcgcg ggctgcaccg cccgatcatg gtggccagtc cgttcttcag ggaacc
//
LOCUS       DASWPO010000308         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2931400, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000308 DASWPO010000000
VERSION     DASWPO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2931400"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..484)
                     /locus_tag="VGC69_16115"
     CDS             complement(128..484)
                     /locus_tag="VGC69_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEY0296854.1"
                     /translation="MKITLYGLTKCSTCIKARDWLDAHGVKHEFIDYRDNPVPAATLK
                     EWAGKVGGWEKLVNRTSMTWRALPEERRAAATDAQWTRLIAEYPALVRRPVTLTADGE
                     VTVGFPEKRYGERFAA"
     gene            complement(481..1284)
                     /locus_tag="VGC69_16120"
     CDS             complement(481..1284)
                     /locus_tag="VGC69_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0296855.1"
                     /translation="MTGPNPFLRALALTLGYGFLYVPIACLVVFSFNDSAVLTSWSGF
                     SLRWYAALTHDDALLSAALLSFKIALLTATAATLIGTWAGYVLARMGRFRGFALYVGM
                     LSAPLVIPEVVLGISLLLMFVEMRGLLGWPEENGSFTIWVGHVTLCMAYVAVIIQARV
                     RDLDRSLEEAALDLGASPLKVFFTITLPLIVPSLVSAWLLSFTLSLDDVVIASFLSGP
                     GASTLPMEVFSRVRLGLKPEVNALATLFILAVGLFVVVANRLQWRKEKT"
     gene            complement(1281..>2107)
                     /locus_tag="VGC69_16125"
     CDS             complement(1281..>2107)
                     /locus_tag="VGC69_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0296856.1"
                     /translation="LVLKISFADLQFGIPPYTSLVEYKDQALQFSLHLRGYALLFTDS
                     LYFATYLSSVKIAAISTLVCVLIGYPMAYYIARSAPATRNLLLLGVILPFWTSLLLRV
                     YAWVGILRNDGLLNKLLMALGLTAAPLEIYRTDLAVYIGMVYAYLPFFVLPLYANLVK
                     TDLRLLEAAYDLGARPWSAFWRITLPLSMPGVVSGAMLVFIPCVGEYVIPEMLGGANT
                     LMMGRVMWNEFFNNADWPMASAVTCVMVVVLLVPLALFQYSQARNQDREQAKGAGR"
BASE COUNT          427 a          687 c          707 g          286 t
ORIGIN      
        1 gggccgaggg cgcctgcatg cgcccgagcc cggcacagcg aagccccggg cgtagggccg
       61 cctcggccag gacggccgca agaacggcat caggccgcaa gaacagcgtc agcccacaag
      121 ccccgcatca agccgcaaac ctctccccat accgcttctc gggaaacccc acggtcactt
      181 cgccatcggc cgtgagcgta accggccggc gcaccagggc cggatattcg gcaatgagcc
      241 tggtccattg ggcatcggtg gcggcagcgc ggcgctcctc gggcagcgcg cgccaggtca
      301 tggaggtgcg gttgaccagt ttttcccaac cgccgacctt gccggcccac tccttgagcg
      361 tggccgcggg aaccgggttg tcgcggtaat cgatgaactc gtgcttgacg ccgtgcgcgt
      421 cgagccagtc gcgcgccttg atgcaggtgc tgcacttggt caggccatag agcgtgattt
      481 tcatgttttt tccttgcgcc attgcaggcg attggccacg accacgaaga ggccgacggc
      541 caggatgaac agcgtggcca gggcattgac ctcgggcttg aggcccaggc gcacgcgcga
      601 gaagacttcc atgggcagcg tggacgcgcc cggccccgac aggaaggagg cgatcaccac
      661 atcgtccagc gacagggtga aggacaagag ccaggccgac accagcgagg gcacgatgag
      721 cggcagggtg atggtgaaga acaccttaag cggcgacgcc cccaggtcga gcgcggcctc
      781 ttcgagcgag cggtcgaggt cgcgcacgcg cgcctggatg atcaccgcca cataggccat
      841 gcacagggtg acgtggccca cccagatggt gaagctgccg ttttcctcgg gccagcccag
      901 gaggccgcgc atctcgacga acatgagcag gagcgaaatg cccagcacca cctcgggaat
      961 gaccagcggc gcgctcagca tgcccacgta cagggcgaag ccgcgaaagc gccccatgcg
     1021 cgccagcaca tagcccgccc aggtgccgat gagggtcgcc gccgtggccg tgagcagcgc
     1081 gatcttgaaa gacagcagcg cggccgacag cagcgcgtcg tcatgcgtca aggcggcgta
     1141 ccagcgcagc gagaagccgc tccatgacgt cagcaccgcc gaatcgttga acgaaaacac
     1201 caccaggcag gcgatgggca cgtacaaaaa gccgtagccc agcgtcaggg ccagcgcgcg
     1261 caggaacggg ttgggcccag tcatcgcccg gctccctttg cctgctccct gtcctgattg
     1321 cgcgcctggc tgtactggaa cagggccagc ggcaccagca gcaccaccac catgacgcag
     1381 gtcacggccg aggccatcgg ccagtcggcg ttgttgaaga actcgttcca catcacgcgg
     1441 cccatcatca gcgtgttggc gccgcccagc atttcgggga tgacgtactc gcccacgcag
     1501 gggatgaaca ccagcatggc gcccgagacc acgcccggca tggacagggg cagcgtgatg
     1561 cgccagaagg cgctccaggg ccgggcgccc aggtcgtagg cagcctcgag caggcgcagg
     1621 tccgtcttga ccaggttggc gtagagcggc aggacgaaga agggcaggta cgcatagacc
     1681 atgccgatat acacggccag atcggtgcgg tagatctcga gcggcgcggc ggtcaggccc
     1741 agcgccatca gcagcttgtt gagcaggccg tcgttgcgca ggatgcctac ccacgcgtag
     1801 acgcgcagca gcagcgaagt ccagaacggc aggatcacgc ccagcagcag caggttgcgg
     1861 gtggccggcg ccgaccgggc gatgtaatac gccatgggat agccgatcaa cacgcacacc
     1921 agcgtgctga tggcggcgat cttcaccgaa ctcaggtagg tggcgaaata caggctgtcc
     1981 gtgaacagca gcgcgtagcc gcgcaggtgc aggctgaact gcagggcctg gtccttgtac
     2041 tccaccagcg acgtataggg cgggatgccg aactgcaggt cggcgaagct gatcttcagg
     2101 accagca
//
LOCUS       DASWPO010000309        10054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3108603, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000309 DASWPO010000000
VERSION     DASWPO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3108603"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VGC69_16130"
     CDS             complement(<1..578)
                     /locus_tag="VGC69_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEY0296857.1"
                     /translation="MTAVSDALPVRPPLIGKAELITRAFQKKDLGALQRELVARLERD
                     PDDAHAMLDLSMVLLLNGQREQALAMQGYALQAQQLYYMPARRQPATMTLLALVSGGD
                     LMANTPLEFLIANASIDLCLLYLGPGVPAPKTLPAHDLVMVAVGETTGNRELLQGLVG
                     PLSRWHTPVLNRPGHVLKTSRDGLHAALRHAPG"
     gene            complement(575..2110)
                     /locus_tag="VGC69_16135"
     CDS             complement(575..2110)
                     /locus_tag="VGC69_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0296858.1"
                     /translation="MEHPMKGNAAGAVSSPDDLSPSLDPADWADWRRQGHQMLDDMFD
                     YLAGLRERPVWQPAPDAVRQRFSETLPREASDLGEVHGRFMRDVLPYTAGNAHPGFMG
                     WVHGGGTAVGMLAETLAAGLNANVGGRNQMPVEVERQITRWMCELFGFPETASGLFVT
                     GTSMANLVGVLVARRAVLGAQVRKRGLAAQAARLAAYTSASAHGCIAQAMDLSGIGTD
                     ALRLVPVDADFRMQPQALRAAIAADRQAGYTPFFIAGTAGTVDVGAIDPLLELADIAR
                     REGVWFHVDGAFGALAMLSPELAPKLAGIEHADSIGFDFHKWGQVPYDAGFVLVRDGQ
                     MHQDTFAANAAYLQRETRGLAGGSPWPCDFGPDLSRSFRALKTWFTLQVYGADKLGAA
                     MANSCRVARHLATRVQVEPALELLAPVDLNIVCFRFRHAQADRINAAIAVALHESGIA
                     APSTTKVGGHLAIRAAIVNHRTSVADVDRMVDAVLCIGYRMASIIGLVDEPAVASPSL
                     LAP"
     gene            2306..3070
                     /gene="trmB"
                     /locus_tag="VGC69_16140"
     CDS             2306..3070
                     /gene="trmB"
                     /locus_tag="VGC69_16140"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY0296859.1"
                     /translation="MNEHSPDQARPPAALSDDVRAALAQAGHAPASPGATHIRSFVHR
                     RGHITQGQRDALEHLLDKWSVSYAPRLLDASAAFGRQAPLILEIGFGMGETTEKIALA
                     RPGDNFLGVEVFNAGVGSLLRRIEESSIPNLRIVQHDAVEVVRDMIAPDSLAGVHVYF
                     PDPWPKKRHHKRRLIQPPFVSLLASRIAPGGYLHCATDWQEYAAQMLAVLGAEPSLRN
                     TAADYAQRPDYRPQTKFETRGLRLGHGVWDLIFQRV"
     gene            3113..4057
                     /gene="pip"
                     /locus_tag="VGC69_16145"
     CDS             3113..4057
                     /gene="pip"
                     /locus_tag="VGC69_16145"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY0296860.1"
                     /translation="MLYPPIEPYRQGMMDTGDGHQIYWELCGNPAGKPAVFLHGGPGS
                     GSSPVHRQLFNPRLYNVLLFDQRGCGRSLPHASLDNNTTWHLTADIERLRTEVMGADR
                     WLAFGGSWGSTLALAYAQQHPEHVSELIVRGIFGLRRAELLWFYQEGASWLFPDHWED
                     YLAPIPPEERGDMIAAYHKRLTSDNPVEQLRAAKAWSRWEDNTITLLPNARHQQSHAA
                     DRAALAFARIENHYFVHDGFMETDGQLLRDAYKLHDIPGTIVQGRYDACTPARTAWDL
                     HKAWPQAVFHLVPDAGHAFDEPGILARLIEATDTYAST"
     gene            4057..4257
                     /gene="thiS"
                     /locus_tag="VGC69_16150"
     CDS             4057..4257
                     /gene="thiS"
                     /locus_tag="VGC69_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY0296861.1"
                     /translation="MQITLNGAPRELPPGLAVTGLLDTLGYTGKRVAVERNGEIVPKS
                     RHADTALQDGDQIEIVVAVGGG"
     gene            4292..4675
                     /locus_tag="VGC69_16155"
     CDS             4292..4675
                     /locus_tag="VGC69_16155"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0296862.1"
                     /translation="MIDTVLTPKAPAPGGHYSQAVAANGFVFVAGQLPFTVDPPGQIP
                     AGIEAQTRQALENVRQVLEAAGSGLDRLVSVTVFVTDMADWPEVNRVYADVLGAHRPA
                     RAVAVSPQLHHGCLVEILATALAGR"
     gene            4688..6082
                     /locus_tag="VGC69_16160"
     CDS             4688..6082
                     /locus_tag="VGC69_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296863.1"
                     /translation="MLALDRRTAIALLAAGVFFMENLDATIITTALPAMAKEFGVLPA
                     QLSVGVSAYLVALTMFIPASGWAADRYGARRVFPVAIIVFTLASLLCAASGSLLGFTL
                     ARILQGLGGAMMVPVGRLAVLRNTPKEGIVRALAILTWPGLVAPILGPPVGGWISMYW
                     SWHWIFLINLPLGLVALFAAWVLIRDDEERSNRFDLPGFVLSGLGFAFFMSGIELASR
                     ADLPSAPAVVTLAAGLVLLGVSAWHLRRVDHPLFGLAPLAIQTFRVTAVGGSLFRTAI
                     GSAPFLLPLMFQLGFGYSAVESGILLLWLFAGNLCIKPATTAIMHRFGFRTVLVVNGL
                     LVAAGFAGFALLQADTPRAVTIALLFFSGVTRSMQFTALNTIGFSDVPPPQMRDATTL
                     FSVLQQMNAGMGIAVGALALSAAERLRDTAAGMVGTGDFQLAFWLLAALALAAVVDCL
                     MLPRDAGQRVLAPR"
     gene            complement(6083..7486)
                     /gene="ffh"
                     /locus_tag="VGC69_16165"
     CDS             complement(6083..7486)
                     /gene="ffh"
                     /locus_tag="VGC69_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY0296864.1"
                     /translation="MLDNLTQRLSRVVKTLRGEARLTEANTQEMLREVRMALLEADVS
                     LPVVRDFVARVKEKALGEEVSGSLTPGQALVGVVHTELTSLMGGDLGPGAGELSLAVQ
                     PPAVILMAGLQGAGKTTTTGKLARWLAEGGHTSNGRPTGKKKVLVVSADVYRPAAIDQ
                     LKAVAAQAGVDFLPSDPSQKPEDIASNAVDHAKRHHYDVLILDTAGRLGIDEAMMREI
                     RALYDLVKPIETLFVVDAMQGQDAVNTARAFADALPLTGVVLTKLDGDARGGAALSVR
                     HVTGKPLKFVGVSEKLDGLEPFHPDRMAQRVLGMGDIVSLVEQAQKNIDIAEAQKLAA
                     KIKSGNKFDLNDFRDQLGQVKKMGDMSSLLEKLPAQLQQAAGQLQGGQAEKQLRRTEG
                     ILNSMTPAERSKPELIKAARKRRIAAGAGVPVQEVNKMLNQFEQMQGMMKMMKKGGMG
                     KMMRAMGAMKGAGRFGR"
     gene            7536..8378
                     /gene="ccsA"
                     /locus_tag="VGC69_16170"
     CDS             7536..8378
                     /gene="ccsA"
                     /locus_tag="VGC69_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY0296865.1"
                     /translation="MSLGIVFHAAAALAYALLGLGNTRRLLRNGSAERVGQTARLCLL
                     GALALHAAALYLSLQDDGQLFIGWALALSAAVWLGLVVYWIEGFFIRLDGLQLLLIPA
                     ALLTTLLAALFPQGIIVPHVGNTWLRAHLVISLAAYGLITIAALQAVLMALLDRHLRQ
                     TANPATQRKLVARVLDVQPPLLVQERLLFRLIWVGFGMLTLAVVTGSVASLSLTDRVL
                     PFDHKTVFTLLSWATFGGLLLGRRLQGWRGRVALRWTLTGFVFLILAYTGSRFVLEVI
                     LHRS"
     gene            8406..8663
                     /locus_tag="VGC69_16175"
     CDS             8406..8663
                     /locus_tag="VGC69_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEY0296866.1"
                     /translation="MKFLIWFLIILAGLTATRLFNARQARRTAPQPQPQPARTDSGAE
                     HMVRCARCGIYLPRSEALLIGQRTWCCEDHARLGPDLNQQP"
     gene            8665..9246
                     /gene="ampD"
                     /locus_tag="VGC69_16180"
     CDS             8665..9246
                     /gene="ampD"
                     /locus_tag="VGC69_16180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEY0296867.1"
                     /translation="MPSLQLDRHGWLRPGPSVQCLPSPNVDARPAGTSVSLLVIHNIS
                     LPPNRFEGPCVADLFLNRLDYGSHPWLEQLRGLRVSAHFFVRRDGSVLQFAATQSRAW
                     HAGVSRFQGRERCNDFSIGIEVEGADTVPYDDRQYATLAQLAPLLRARHPLAHVRGHE
                     HIAPGRKTDPGPAFDWQRFGRESGYARRAMPPR"
     gene            9286..9918
                     /locus_tag="VGC69_16185"
     CDS             9286..9918
                     /locus_tag="VGC69_16185"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0296868.1"
                     /translation="MLKIWGRASSINVQKVMWAVRELALPHERLEAGGQFGVVDTPEY
                     IRMNPNRMVPVIEDGGFVLWESNAIVRYLAARYGAGSLWPTDPCVRGDSDRWMDWQTT
                     EWQPSMLQAFLGLVRTPADQRDHAAVAASLTRANAKAMVLEQMLQGREFVAGSHFTMG
                     DIVLGCAAHRWLGLPVGSPTDRPAVPALSAWYRRLMMRPAVQGVLTLPLK"
BASE COUNT         1457 a         3546 c         3384 g         1667 t
ORIGIN      
        1 cccggcgcat ggcgcaaggc ggcgtgcagg ccgtcgcgcg aggtcttgag cacgtgcccc
       61 gggcggttga gcaccggcgt atgccaccgg ctcaacgggc cgaccaggcc ttgcagcagc
      121 tcgcggttgc cggtggtctc gcccacggcc accatgacca ggtcgtgcgc cggcaaggtc
      181 ttgggcgccg gcacgcccgg ccccaggtac aacaggcaca gatcgatact ggcgttggcg
      241 atcaggaatt ccagcggcgt gttggccatc aagtcgccgc cgctcaccaa ggccagcagc
      301 gtcatggtgg cgggctgccg gcgcgccggc atgtaataga gctgctgcgc ctgcagggcg
      361 tagccctgca tggcgagcgc ctgctcgcgc tggccgttga gcagcagcac catcgacagg
      421 tcgagcatgg cgtgcgcatc gtcggggtcg cgttcgaggc gggcgacgag ctcgcgctgc
      481 aatgccccca ggtctttttt ctggaaagcg cgcgtgatga gttcggcctt gccgatcagg
      541 ggcggccgaa ccggcagggc gtcggatacg gcagtcatgg agcgagcaaa gacggggagg
      601 cgacggcggg ttcatcgacc agcccgatga tgctggccat gcgatagccg atgcagagca
      661 cggcatcgac catccggtcg acgtcggcca cgctggtgcg atggttgacg atggccgcgc
      721 ggatggccag atggccgccg accttggtgg tggacggcgc ggcaatgccg gactcatgca
      781 gggccaccgc gatggccgcg ttgatgcggt cggcctgcgc atggcgaaag cggaagcaca
      841 cgatgttcag gtccacgggc gccagcagct cgagcgccgg ttcgacctgc acgcgcgtgg
      901 ccaggtggcg cgccacccgg cagctattgg ccatggcggc gcccagcttg tcggcgccgt
      961 acacctgcag ggtgaaccag gttttcaggg cgcggaaact acgcgacagg tccggcccga
     1021 agtcgcaagg ccagggcgat ccgccggcca ggccgcgcgt ttcgcgctgc agataggcgg
     1081 cgttggcggc aaaggtgtcc tggtgcatct gcccgtcgcg caccagcacg aagccggcgt
     1141 cgtaaggcac ctgcccccat ttgtggaagt cgaagccgat ggaatcggca tgttcgatgc
     1201 ccgccagctt gggggccagc tcgggcgaca gcatggccag cgcgccgaag gcgccgtcca
     1261 catggaacca caccccttcc cgcctggcga tgtcggccag ttcaagcaag gggtcgatgg
     1321 cgccgacgtc cacggtgccg gcggtgccgg cgatgaaaaa aggcgtgtag cccgcctgcc
     1381 tgtcggcggc gatggcggcc cgcagggcct ggggctgcat gcggaaatcg gcgtcgaccg
     1441 gaaccaggcg caaggcatcg gtgccgatgc cggagaggtc catggcctgc gcgatgcagc
     1501 cgtgcgcgct ggccgaggtg taagccgcca ggcgcgccgc ttgcgcagcc aggccgcgct
     1561 tgcgcacctg ggcgcccagc accgcgcggc gcgccaccag cacgccgacc aggttggcca
     1621 tggaggtgcc cgtgacgaac aggccgctgg ccgtctcggg aaaaccgaag agttcgcaca
     1681 tccagcgcgt gatctggcgc tcgacctcga ccggcatctg gttgcggccg cccacattgg
     1741 cattgagccc ggcggccagg gtttcggcca acatgcccac ggccgtgccg ccgccatgca
     1801 cccagcccat gaagccgggg tgcgcattgc ccgccgtgta cggcaggacg tcgcgcatga
     1861 agcggccgtg cacctcgccc aggtcggaag cctcgcgcgg cagcgtttcg gaaaagcgct
     1921 gccgcacggc gtcgggcgcg ggctgccaca cgggacgctc gcgcaggccg gccaggtagt
     1981 cgaacatatc gtcgagcatc tggtggccct ggcgtcgcca gtccgcccaa tcggcgggat
     2041 ccagggaggg ggacagatcg tccggcgacg aaacggcgcc ggccgcgttt cctttcatgg
     2101 ggtgttccat ggggcgttcc ttgctgcgcg ctcctcgggc agcctggtgg gaatcttgcc
     2161 acccgccccc gccggcaaat gcgatgaacg cgagcccaca tgggggtcat ttcggaggtt
     2221 tgccgccagg gaccgtcgcg gcgcggtttg cccatatatt agggacgtat cttaaggcgc
     2281 acggcagccg actacaattc ggcccatgaa cgagcattcc cccgatcaag cccggccgcc
     2341 ggccgcattg tccgacgacg tgcgggcggc gctggcccaa gccggccacg cgcccgccag
     2401 cccgggcgcc acgcatatcc gcagtttcgt gcatcgccgc gggcacatta cccagggcca
     2461 gcgcgacgcg ctggaacacc tgctggacaa atggtccgtc tcctatgcgc cgcgcctgct
     2521 cgacgcaagc gccgccttcg gccgccaggc gccgctgatc ctggagatcg gcttcggcat
     2581 gggcgagacc accgaaaaaa tcgccctggc ccgtcccggg gacaatttcc tgggcgtgga
     2641 ggtcttcaac gccggcgtgg ggtctctcct gcgccgcatc gaggagtcgt ccatccccaa
     2701 cctgcgcatc gtccagcacg acgccgtgga agtggtgcgc gacatgatcg ccccggattc
     2761 gctggccggc gtgcatgtgt attttcccga cccctggccc aagaagcgcc accacaagcg
     2821 ccgcctgatc cagccgcctt tcgtgtcgct cctggccagc cggattgccc ccggcggcta
     2881 cctgcactgc gccaccgact ggcaggaata cgccgcgcag atgctggcgg tgctgggcgc
     2941 cgaacccagc ctgcgcaaca ccgcggccga ctacgcgcag cgccccgact accgcccgca
     3001 gaccaagttc gagacccgcg gcctgcgcct gggccacggg gtatgggacc tgatcttcca
     3061 gcgcgtctga ccgcgcggca tgacgcaatc aagcaagctt cgaggaaacc gcatgctgta
     3121 cccgccgatc gaaccctacc gccagggcat gatggacacc ggcgacggcc accagatcta
     3181 ctgggaactg tgcggcaacc cggcgggcaa gcccgcggtg ttcctgcatg gcggccccgg
     3241 cagcggcagt tccccggtgc ataggcagct gttcaatccc aggctgtaca acgtgctgct
     3301 gttcgaccag cgcggctgcg gccgctcgct gccgcatgcc agcctggaca acaacaccac
     3361 ctggcacctg acggccgaca tcgagcgcct gcgcaccgag gtcatgggcg cggacaggtg
     3421 gctggcgttc ggcggctcct ggggctcgac cctggcgctg gcctatgcgc agcaacaccc
     3481 cgaacacgtc agcgagctga tcgtgcgcgg catcttcggc ctgcgccgcg cggagctgct
     3541 gtggttctac caggaaggcg cctcgtggct cttccccgac cactgggaag actacctcgc
     3601 gccgattccg cccgaggaac gcggcgacat gatcgcggcc tatcacaagc ggctcaccag
     3661 cgacaatccg gtcgagcagc tgcgcgcggc caaggcctgg agccgctggg aagacaacac
     3721 catcaccctg ctgcccaacg cccggcacca gcagagccac gcggccgacc gggccgcgct
     3781 ggccttcgcc cgcatcgaga accattattt cgtgcacgac ggtttcatgg aaaccgacgg
     3841 ccagctgctg cgcgacgcct acaagctgca cgatattccc ggcaccatcg tccagggccg
     3901 ctacgatgcc tgcacgccgg cccgcacggc ctgggacttg cacaaggcct ggccgcaggc
     3961 cgtcttccac ctcgtgcccg atgccggcca tgccttcgac gagccgggca tcctggcgcg
     4021 cctgatcgaa gccaccgaca cctacgcgag cacctgatgc agatcaccct caatggcgcc
     4081 ccgcgcgaac tgcccccggg cctggccgtg accggcctgc tcgacacgct gggctacacg
     4141 ggcaagcgcg tcgccgtgga acgcaacggc gagatcgtgc ccaagagccg gcacgcggac
     4201 accgccttgc aggacggcga ccagatcgag atcgtggtgg ccgtcggcgg cggctgaccc
     4261 ggccacgcga ccgacgcgac aaggaaccca catgatcgat accgtcctca cccccaaggc
     4321 ccccgcgccc ggcggccact acagccaggc cgtcgccgcc aacggcttcg tgttcgtggc
     4381 ggggcagttg cccttcacgg tggacccgcc cgggcagatc ccagcaggca tcgaggcgca
     4441 gacccgccag gcgctggaga acgtgcgcca ggtgctggaa gcggccggct cgggcctgga
     4501 ccgcctggtc agcgtcacgg tgttcgtgac cgacatggcc gactggcccg aggtcaaccg
     4561 cgtgtacgcc gacgtgctgg gcgcgcatcg ccccgcgcgg gccgtggcgg tatcgccgca
     4621 actgcaccac ggctgcctgg tggagatcct ggccacggcg ctggccggcc ggtaaaccgg
     4681 gaacgccatg ctcgcgctgg atcgacgcac cgccatcgcc ctgctggccg caggcgtgtt
     4741 cttcatggag aacctggacg ccaccatcat caccacggcc ctgccggcca tggcgaagga
     4801 attcggcgtt ctgcccgccc agctgtcggt cggcgtgtcg gcctacctgg tcgcgctgac
     4861 catgttcatc ccggccagcg gctgggcggc cgaccgctac ggcgcccgcc gcgtgttccc
     4921 ggtggccatc atcgtcttca ccctggcctc gctgctgtgc gcggccagcg gcagcctgct
     4981 cggctttacc ctggcgcgca tcctgcaggg cctgggcgga gccatgatgg tgccggtggg
     5041 gcgcctggcc gtgctgcgca atacgcccaa ggaaggcatc gtgcgggccc tcgccatcct
     5101 gacctggccg ggactggtcg cccccatcct ggggccgccg gtgggcggct ggatcagcat
     5161 gtactggagc tggcactgga tcttcctgat caacctgccc ctgggtctcg tcgcgctgtt
     5221 cgccgcctgg gtgctgatcc gcgacgacga ggaacgcagc aaccgcttcg acctgccggg
     5281 attcgtcttg agcggcctgg gtttcgcctt cttcatgagc ggcatcgaac tggccagccg
     5341 tgccgacctg ccctccgcgc ccgcggtcgt gacgctggcg gccgggctcg tcctgctggg
     5401 ggtttcggcc tggcacctgc gacgcgtcga ccatcccctg ttcggcctgg cgccactggc
     5461 gatccagact ttccgcgtca ccgcggtggg cggctcgctg tttcgcaccg cgatcggcag
     5521 cgcgcctttc ctgctgccgc tgatgttcca gctgggcttc ggctacagcg cggtggagtc
     5581 gggcatcctg ctgctgtggc tgttcgccgg caacctgtgc atcaagcccg ccaccacggc
     5641 catcatgcac cgcttcggtt tccgcaccgt gctggtggtc aacggcctgc tggtggccgc
     5701 gggcttcgcc ggctttgccc tgctgcaggc cgacacgccc cgcgcggtga cgatcgcgct
     5761 gctctttttc agcggcgtga cccgctcgat gcagttcacc gcgctcaaca ccatcggttt
     5821 ctcggacgtg ccgccgccgc agatgcgcga cgccaccacg ctgttctcgg tgctgcagca
     5881 gatgaacgcc ggcatgggca tcgccgtggg cgcgctggcc ctgtcggcgg ccgaacgcct
     5941 gcgcgacacg gcggccggca tggtgggcac gggggatttc cagctggcct tctggctgct
     6001 ggcggcgctg gcgctggccg cggtcgtcga ctgcctgatg ctgccgcgcg acgcgggcca
     6061 gcgcgtgctg gcgccgcgct gatcagcgcc cgaagcggcc cgcgcccttc atggcgccca
     6121 tggcgcgcat catcttgccc atgccgccct tcttcatcat cttcatcatg ccctgcatct
     6181 gctcgaactg attgagcatc ttgttgactt cctgcacggg cacgccggcc cccgccgcga
     6241 tgcggcgctt gcgcgcggcc ttgatgagtt cgggcttgga gcgctcggcc ggcgtcatgg
     6301 aattgaggat gccctcggtg cggcgcagct gcttctcggc ctggccgcct tgcagctggc
     6361 cggccgcctg ctgcagttgc gccggcagct tctcgagcag cgagctcatg tcgcccatct
     6421 tcttgacctg gccgagctgg tcgcggaagt cgttgaggtc gaacttgttg cccgacttga
     6481 tcttggccgc gagcttctgc gcctcggcga tgtcgatgtt cttctgcgcc tgctccacca
     6541 gcgacacgat gtcgcccatg cccagcacgc gctgcgccat gcggtcgggg tggaaaggct
     6601 ccaggccgtc gagcttctcg gagacgccca cgaacttgag cggcttgccg gtcacgtgcc
     6661 gcaccgacag ggccgcgccg ccgcgcgcat cgccgtcgag cttggtgagc accacgccgg
     6721 tcagcggcag cgcgtcggca aaggcgcgcg cggtgttgac cgcgtcctgg ccctgcatgg
     6781 cgtcgaccac gaacagcgtt tcgatgggct tgaccaggtc gtacagcgcg cggatctcgc
     6841 gcatcatcgc ctcgtcgatg cccaggcggc cggccgtatc gaggatcagc acgtcgtagt
     6901 ggtggcgctt ggcgtgatcc accgcgttgc tcgcgatgtc ctcgggcttc tggctcgggt
     6961 cggaaggcag gaagtccacg cccgcctgcg cggccacggc cttcaactga tcgatggcgg
     7021 cagggcggta gacgtcggcg ctcaccacca ggactttttt cttgccggtg ggacggccgt
     7081 tgctggtgtg gccgccctcg gccagccagc gggccagctt gccggtggtg gtggtcttgc
     7141 ccgcgccctg caggccggcc atgaggatga cggccggcgg ctgcacggcc agcgacagtt
     7201 cgccggcgcc gggaccgagg tcgccgccca tgaggctggt gagctcggtg tgcaccacgc
     7261 ccaccagggc ctggccgggc gtgaggctgc cggagacctc ttctcccagc gccttttctt
     7321 tgacgcgggc cacgaagtcg cgcaccaccg gcagcgacac gtcggcctcc agcagggcca
     7381 tgcgcacttc gcgcagcatc tcctgggtgt tggcctcggt caggcgtgct tcgccgcgca
     7441 gggtcttgac gacgcgcgaa agtcgttgag tgaggttatc gagcatgtgg gaaaggacgt
     7501 tacgcttaaa ctagccggag gaaccggaac cttttatgtc actgggtatt gtatttcacg
     7561 ccgccgcggc cctggcttac gccctgctgg gcctcggcaa cacccgccgc ctgctgcgca
     7621 acggcagcgc cgaacgggtc ggccagacgg cccggctgtg cctgctgggc gccctggcgc
     7681 tgcacgccgc cgccctgtac ctgagcctgc aggacgacgg ccagctcttc atcggctggg
     7741 ccctggccct gtcggccgcc gtgtggctgg ggctggtggt gtactggatc gagggctttt
     7801 tcatccgcct cgacggcctg cagctgctgc tgataccggc cgccctgctg accaccctgc
     7861 tggcggcgct gtttccgcaa ggcatcatcg tgccgcatgt cggcaacacc tggctgcgcg
     7921 cccacctggt catttcgctg gccgcctacg gcctgatcac catcgccgcc ctgcaggccg
     7981 tgctcatggc cctgctcgac cggcatctgc gccagaccgc gaatccggcc acgcagcgca
     8041 agctggtggc ccgcgtgctc gacgtccagc cccccctgct ggtgcaggaa aggctgctgt
     8101 tccggctgat ctgggtcggc tttggcatgc tgacgctggc ggtggtcacc ggctcggtcg
     8161 cttcactgtc gttgaccgat cgcgtgctgc ctttcgacca taaaaccgtg ttcaccctgc
     8221 tatcctgggc aacgttcggc ggattgctgc tggggcgccg cctgcaaggc tggcgcggcc
     8281 gcgtggcgct gcgctggacg ctgaccggct ttgtcttcct gattcttgcc tatacgggca
     8341 gccgattcgt gctggaagtg attctgcatc gaagctgaca tgcgacgccc cccacgttca
     8401 ctatcatgaa attcctgatc tggttcctca tcatcctggc ggggctgacc gccacgcgcc
     8461 tgttcaatgc ccggcaggcc cgccgcacgg cgccccagcc ccagccccag cccgccagga
     8521 ccgacagcgg cgccgagcac atggtgcgct gcgcccgctg cggcatctac ctgccgcgct
     8581 ccgaagccct gctcatcggc cagcgcacct ggtgctgcga agaccacgcc cggctgggcc
     8641 ccgatctcaa ccagcagccc tgacatgccc agcctgcaac tggatcgcca cggctggctg
     8701 cggcccggcc cctcggtgca atgcctgccc tcgcccaatg tggatgcccg gccggccggc
     8761 acgagcgtgt cgctgctggt catccacaac atcagcctgc cgcccaaccg gttcgaaggc
     8821 ccctgcgtgg ccgacctgtt cctgaaccgg ctcgactacg gctcgcaccc ctggctggaa
     8881 cagctgcgcg gcctgcgggt gtcggcgcat ttcttcgtgc gccgcgacgg cagcgtgctg
     8941 cagttcgccg cgacgcaatc gcgggcctgg catgccggcg tgtcgcgctt ccagggccgc
     9001 gagcgctgca atgatttttc catcggcatc gaggtcgagg gcgcggacac cgtgccctat
     9061 gacgaccggc agtacgccac cctggcgcaa ttggcgccgc tgctgcgcgc gcgccacccc
     9121 ctggcccacg tgcgcggcca cgagcacatc gcgcccggcc gcaagaccga ccccggcccg
     9181 gctttcgact ggcagcgctt cggccgcgaa agcggttatg ctcgccgggc catgccgccg
     9241 cgctgaacct tcgccgccgc gcgtggccac ttatcgggaa tcatcatgtt gaagatctgg
     9301 ggccgtgcga gttcgatcaa tgtgcagaaa gtcatgtggg ccgtgcgcga actggcgctg
     9361 ccgcacgaac gcctcgaggc cggcgggcag ttcggcgtgg tcgacactcc cgaatacatc
     9421 cgcatgaatc ccaatcgcat ggtgcccgtg atcgaggacg gcggctttgt gctgtgggaa
     9481 tcgaatgcca tcgtccgcta cctggccgcg cgctatggcg cgggctcgct gtggcccacc
     9541 gacccttgcg tgcgcggcga ctccgaccgc tggatggatt ggcagaccac ggaatggcag
     9601 ccgtccatgc tgcaggcttt cctggggctg gtccgcacgc cggcggacca gcgcgatcat
     9661 gccgccgttg cggcatcgct cacccgcgcc aatgcgaagg cgatggtgct ggaacagatg
     9721 ctgcaaggca gggagttcgt tgccggatcg catttcacga tgggggacat cgtgctggga
     9781 tgcgcggcgc atcgctggct gggactgccg gtcgggtcgc cgaccgatag gccggccgtg
     9841 ccggctttgt cggcgtggta tcgaaggctg atgatgcggc cggcggtgca gggggttttg
     9901 actttgccgc tcaaatgagc aaacagccgc tttttacggg cggctgtttg tttgggcgag
     9961 ttcgtttggg ttcttaacgc gtcagccggt ccgggcttct cctcgcagcc tgatcggcgg
    10021 ggctcgggcg cgccatcagg cgccctcggc ccct
//
LOCUS       DASWPO010000310         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1682736, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000310 DASWPO010000000
VERSION     DASWPO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682736"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGC69_16190"
     CDS             complement(<1..213)
                     /locus_tag="VGC69_16190"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0296869.1"
                     /translation="MDYPELVLQINGRLCGVQGRETIEVVDPATEAVLGLLPKATPAD
                     VEEALQAAGAAFHVWRKTPALDRAKVL"
     gene            complement(216..1187)
                     /locus_tag="VGC69_16195"
     CDS             complement(216..1187)
                     /locus_tag="VGC69_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296870.1"
                     /translation="MKRIVFKAMLCLLAAIAMPAMAAYPDRPITLIIGYTAGGPTDIV
                     GRYLAKGLERELGQSVIVENRPGANAVVALLAVKKAAPDGYTLYLGSSGTLSIEPVYK
                     KRVNYDVLKDLTPVGLVASYPYLLVVPQDSPYKTVQDLVAGARAQPGKLSFASAGSGA
                     VNHLAGEWFKSATNTQITHIPYKGDSDAIADLVAGRVDMAFLSAIAAMPQIRANKFRV
                     LAIASAEPSEVMPGMVTVAQAASVPGFAAEPWNGVLAPAGLPLEIVNKLNAAINKVMS
                     TPDAHKTLLGLGQTPLADTPAALAQRITEQTARWDDVIKTGHIEQGE"
     gene            complement(1240..2133)
                     /locus_tag="VGC69_16200"
     CDS             complement(1240..2133)
                     /locus_tag="VGC69_16200"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0296871.1"
                     /translation="MKAETLPLGFIGLGVMGEPMCANLVRKSGHPVYVADLNKEPVAR
                     LAELGAKPCGSIIEVARSAEIVFLSLPSIVQVEQACLGPSGIVEAAGKVRTVVDMSTS
                     DVTRTRSLAEKLKTHGIELIDAPVARSREAARLGTLMISVGGAQAQYEAVKPYLSCMG
                     SDVLHCGGTGNGQVVKIMNNMVLFMTVHALAEAVTIARHAGVDATLLLEALSKGSADS
                     FALRVPGLKALAPDAFPEKTFPTEYAIKDILLALDLAKQGEVDARAAKLTHGLLEQTR
                     EAGFAKAYYPVFIKLIENGYR"
     gene            complement(2389..3303)
                     /locus_tag="VGC69_16205"
     CDS             complement(2389..3303)
                     /locus_tag="VGC69_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296872.1"
                     /translation="MRLDLADLRLFLCVVDAGSITQGAARANLALASASERLRSIEAD
                     AGVALLERRPRGVVATQAGEALAHHARLILRQQALLRGELRDFAAGARGTLRLYANTA
                     ALTEFLPHRLAPWLAERPRLHVELKERTSAEIVRTLSAGLAEAGIVSDAVEAAGLQRQ
                     PVAQDHLVLIVHAGHRLAQNGSVRFADVLGEPFVGMAPGNALQDHIDDHARAAGRPLA
                     LRICMKTFEGLCEMVAHGVGLGIVSQGIARRHRRRYGYEVLPLADDWARRRLCLCFRE
                     WEGLSSPMQNLLIHLGAVPTPLPLPKRP"
     gene            3398..4144
                     /locus_tag="VGC69_16210"
     CDS             3398..4144
                     /locus_tag="VGC69_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0296873.1"
                     /translation="MHQSIVFLGTLALTFLAAGLVKGVTGMGLPTVAMGLLGAVMPPA
                     AAAAMLVTPSFVTNVWQLLAGPAAMPLLRRLGLMLLGIVLGTVGGSALLVRVDPQWSG
                     LALGVALMGYAGHALVSPALSIPPRLEPWLSPLAGLATGAITGATGVFVMPAVPYLQA
                     LRLDKDELVQALGLSFTVSTVALAAGLAAHHAFRLDQLGLSALAIVPALAGMWLGQKI
                     RARISPRRFRQGFLAFLVLLGLELVSRPFF"
     gene            complement(4153..5028)
                     /locus_tag="VGC69_16215"
     CDS             complement(4153..5028)
                     /locus_tag="VGC69_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296874.1"
                     /translation="MTASSVHLIRPEFEHELEPLPAHVSVAVPLSWHERLWQNTAVRK
                     LSILLMLVVFWEAVARYTDDPLLLPSASQTAAAFLDSLLSGELGMRTGLSLVVLLKGY
                     AAGVLLAFVLTTLAVSTRFGRDLLSTLTSMLNPLPAIALLPLAMLWFGLGQGSLVFVL
                     VHSVLWALALNMYTGFLGVSETLRMAGRNYGLGGLRYVLLILVPAALPAILSGLKIAW
                     AFAWRTLIAAELVFGASSGKGGLGWYIFQNRNELNTDRVFAGLVAVVLIGLLVENLVF
                     ELLERHTVRKWGMQR"
     gene            complement(5052..>5453)
                     /locus_tag="VGC69_16220"
     CDS             complement(5052..>5453)
                     /locus_tag="VGC69_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0296875.1"
                     /translation="ADAYPHTLSGGMKQRVAIARALAMQPRILLMDEPFAALDALTRR
                     RMQEELQQLWEASPFTLLFVTHSIDEALALGNRILLLSPHPGRVRAELNAGQFDLHSA
                     GSPAFQAAARRIHDMLFGEPRAVLPAKQAVA"
BASE COUNT          899 a         1758 c         1838 g          958 t
ORIGIN      
        1 cagcaccttt gcgcggtcca gtgcaggcgt cttgcgccaa acatggaacg cagcgcccgc
       61 cgcctgcagg gcttcttcca cgtctgccgg cgtcgccttg ggcaacaggc ccagcaccgc
      121 ctcggtcgcc ggatcgacga cctcgatggt ttcacggccc tgcacgccgc acaggcggcc
      181 gttgatctgc aagacaagtt ccgggtaatc catgttcatt ctccctgttc gatatgaccg
      241 gtcttgatga cgtcgtccca tcgcgcggtc tgctccgtga tgcgctgggc gagcgcggca
      301 ggggtgtcgg cgaggggggt ctggcccagg ccgagcagcg tcttgtgcgc gtctggtgtg
      361 ctcatcacct tgttgatcgc ggcattgagc ttgttcacga tttccaaagg caggccggcc
      421 ggggcgagaa cgccgttcca gggttcggcg gcaaagcccg gcacgctcgc ggcctgggcg
      481 accgtcacca tgcccggcat cacttccgag ggttccgcac tggcaatggc caggacgcgg
      541 aatttattgg cccggatctg cggcatggcg gcaatggcgc tcaggaatgc catgtcgacg
      601 cggccggcca ccaggtcggc gatcgcatcc gaatcgccct tgtagggaat gtgcgtgatc
      661 tgggtgttcg tcgcgctctt gaaccattct ccggccagat ggttcaccgc gccgctgccc
      721 gcggaggcga agctgagctt gcccggctgc gcccgcgcgc cggcgacgag atcctgcacc
      781 gtcttgtaag gcgaatcctg cggcaccacg agcaggtagg gatagcttgc caccagcccg
      841 acaggcgtca ggtccttgag cacgtcgtag ttcacgcgct ttttgtagac cggttcgatt
      901 gatagcgtgc cgctgctgcc caggtatagg gtatagccgt ccggcgcggc tttcttcacc
      961 gccagcaggg ccaccacggc gttggcgccc ggccggtttt ccacgatgac ggactgcccc
     1021 agttcgcgtt ccagtccctt ggccaggtag cggccgacga tgtcggttgg accgcccgcg
     1081 gtatagccga tgatgagcgt aatgggtcga tcgggatagg ctgccatggc gggcatggca
     1141 atggcggcga gcaggcacag catcgccttg aaaacaatac gtttcatatt tttctcacat
     1201 ggattgaacg atcggagggc tcgcggcggg agcgctcgtt cagcggtagc cgttctcgat
     1261 cagcttgatg aagacggggt agtacgcctt ggcgaatcca gcttcgcggg tctgctccag
     1321 caggccatgt gtcaatttcg cggcgcgtgc gtccacctcg ccctgtttgg ccaagtccag
     1381 tgccagcagg atgtccttga tggcgtattc ggttggaaag gtcttttcgg gaaaggcgtc
     1441 cggcgccagg gctttcaggc cggggacgcg cagggcgaag ctgtcggcgg atcccttgga
     1501 cagggcctcg agcagcaagg ttgcgtcgac ccccgcgtga cgggcgatgg tgacggcctc
     1561 ggccagcgcg tgcacggtca tgaacaggac catgttgttc atgattttca ccacctggcc
     1621 gttgcccgtg ccgccacaat gcaggacgtc gctgcccatg caggacagat aaggcttgac
     1681 ggcctcgtac tgcgcttgcg cgccgccaac ggaaatcatc aacgtaccca gccgcgccgc
     1741 ttcgcgcgaa cgcgccacgg gcgcatcgat caattcgatg ccatgggtct tgagcttctc
     1801 ggccagactg cgcgtgcgcg tcacgtcgct ggtgctcatg tcgactacgg tgcggacctt
     1861 gccggcagcc tccacgatgc cactgggacc caggcaggct tgctcgacct gcacgatgct
     1921 gggcagcgac aggaaaacga tctcggccga ccgggccact tcgatgatgg acccgcaagg
     1981 cttggcgccg agttcggcaa ggcgtgccac gggctctttg ttcagatccg ccacgtagac
     2041 cggatgcccc gacttgcgaa ccagattggc gcacatgggt tcacccatca cacccagtcc
     2101 gataaagccc agcggaaggg tttctgcttt catgttgtct cctttatgaa tcattttttt
     2161 gatttctttt ttattttaag gagaccacca atcccggcta tgaaaaaacg tcaacgcaac
     2221 attctcgaat ttcgtcttcc ttgtccgtgc atggatgcag gctttatttt tcaagcctga
     2281 gtcccttgac cgatcgtcat cctaggattg caacaagcag acagacgaca agacacgatg
     2341 tttttgctgc atcgcaatgc cgcagacaac ccttgctgct cgatgctttc atggccgctt
     2401 gggtaaaggt aatggagtcg gcacggcgcc caagtggatg agcaagttct gcatggggga
     2461 agacaagccc tcccactcgc ggaaacacag gcaaagccgc cggcgcgccc agtcgtccgc
     2521 cagggggagg acctcgtagc cgtagcgccg ccggtggcgg cgggcaatgc cctgcgaaac
     2581 gatgcccagg ccaacgccgt gcgcgaccat ctcgcacagc ccctcgaagg tcttcatgca
     2641 aatccgcaac gccaaggggc ggcccgcggc gcgggcatgg tcatcgatat ggtcttgcag
     2701 cgcattgccg ggcgccatgc cgacgaaggg ctcgcccagg acgtcggcga agcgaaccga
     2761 gccgttttgc gcgaggcggt gcccggcgtg cacgatcagg accaggtgat cctgcgcgac
     2821 gggctggcgc tgcaggccag ccgcctcgac cgcgtcggag acgatgccgg cttcggccaa
     2881 ccccgccgaa agggtcctga cgatttccgc actggtgcgc tccttcagct cgacgtgcag
     2941 gcgcggccgc tcggccagcc atggcgcgag ccggtgcggc aggaactccg tcagcgcggc
     3001 ggtgttcgca tacaggcgca gggtgccccg cgccccggcc gcgaaatccc gcagctcccc
     3061 ccgaagcagc gcctgctggc gcaggatcag gcgggcatgg tgggccaggg cttccccggc
     3121 ctgggtggcg accacgccgc gcgggcggcg ctccagcagg gcgacgcccg catcggcttc
     3181 gatgctgcgc agccgctcgc tggcggaggc cagcgccagg ttggcgcgcg cggcgccctg
     3241 ggtaatgctg ccggcatcca ccacgcacag gaataggcgc aggtcggcaa ggtcgagtcg
     3301 catggggtcg cctacatccc cttgccttcg gactatccga agggttcaag gagaaaacca
     3361 cattgtgcca agacgccggc gaggctccaa tacgggcatg catcaatcca ttgttttcct
     3421 gggaacgctg gcgctgacct tcctggccgc cggcctggtc aagggcgtca ccggcatggg
     3481 cctgcccacc gtcgccatgg ggctgctggg cgccgtcatg ccgccggcgg cggccgcggc
     3541 gatgctggtg acgccctcgt tcgtcacgaa cgtctggcag ctgctggccg gaccggccgc
     3601 gatgcccttg cttcggcgcc tagggctgat gctgctgggc atcgtgctgg gcacggtggg
     3661 cggctcggcg ctattggtgc gggtcgatcc gcagtggtcc gggctggcgc tgggtgtcgc
     3721 gctgatgggg tacgccggtc atgccctggt ctcgccggcc ttgtcgatcc cgccgcgcct
     3781 cgaaccctgg ctgtcgcccc tcgccggtct cgccaccggc gcgatcaccg gcgccaccgg
     3841 cgtgttcgtc atgcccgccg tgccctatct gcaggccctg cggctggaca aggacgaact
     3901 ggtgcaggcg ctggggctgt cgttcacggt ctccaccgtg gcgctggccg ccggcctcgc
     3961 ggcgcatcat gcctttcggc tggatcagct gggcctgtcc gcgctggcca tcgtccccgc
     4021 gctggccggc atgtggctgg gacagaagat ccgggcgcgc atcagcccga ggcgctttcg
     4081 acagggtttc ctggcgttcc tggtgctgct ggggctggag ctggtctcac ggcccttttt
     4141 ctgacgccgg ctttatcgct gcatgcccca cttgcgcacg gtgtggcgtt ccagcaattc
     4201 gaacaccagg ttttccacca gcaggccgat cagcaccacg gccaccaggc cggcgaagac
     4261 gcggtcggtg ttgagttcgt tgcggttctg gaagatgtac cagcccaggc cgcccttgcc
     4321 gctggaggcg ccgaacacca gctcggccgc gatcagggtg cgccaggcga aggcccaggc
     4381 gatcttcagg cccgacagga tggccggcag ggcggcgggc accaggatca gcagcacata
     4441 gcgcagcccg cccagcccgt agttgcggcc agccatgcgc agggtttccg acacgccgag
     4501 aaagcccgtg tacatattga gcgccagggc ccacagcacg gagtggacga gcacgaacac
     4561 caggctgccc tggcccaggc cgaaccacag catggccagc ggcagcaggg cgatggccgg
     4621 cagcgggttt aacatggaag tcagggtgga gagcaggtcg cggccgaagc gggtggacac
     4681 ggccagcgtg gtcagcacga aagccagcag cacgccggcg gcataaccct tgagcagcac
     4741 gaccagggac aggcccgtgc gcatgccgag ttcgccgctg agcagactgt cgaggaaggc
     4801 cgcggccgtc tggctggcgc tgggcaggag cagcggatcg tcggtatagc gggccacggc
     4861 ctcccagaac accacgagca tcagcaggat ggatagcttg cgcacggcgg tgttctgcca
     4921 cagccgctcg tgccaggaga gcggcacggc cacgctgaca tgtgcgggca ggggttcgag
     4981 ctcgtgctcg aactcggggc ggatgagatg caccgaactt gcggtcatcg tcgtggtcat
     5041 cagggactcc atcaggcaac ggcctgcttg gccggcagca cggcgcgcgg ctcgccgaac
     5101 agcatgtcgt ggatgcgccg cgccgcggcc tggaaggccg gcgagcccgc gctgtgcagg
     5161 tcgaactggc cggcgttgag ttcggcgcgc acgcggcccg gatgcggcga cagcagcagg
     5221 atgcggttgc ccagcgccag cgcctcgtcg atcgagtgcg tgacgaagag cagggtgaaa
     5281 ggcgaggctt cccacaattg ctgcagttct tcctgcatgc ggcggcgggt cagggcatcc
     5341 agggcggcaa agggctcgtc catcagcagg atgcgcggct gcatggccaa tgcccgggca
     5401 atggccacgc gctgcttcat gccgcccgac agggtgtgcg gataggcgtc ggc
//
LOCUS       DASWPO010000311        11190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_878659, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000311 DASWPO010000000
VERSION     DASWPO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878659"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VGC69_16225"
     CDS             <1..821
                     /locus_tag="VGC69_16225"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0296876.1"
                     /translation="QYGQLLLGRQDEMMADYVGMYTAVPLLGGIETPHPADLDNTGID
                     FRINSAIRYNSPNIGGFTGSALYSGDSTTGTFGQSSTKSFGLNYNNGGLNIAGAYTSV
                     GNPSSLTDALFTSPSNPIAGYGYSQRYQAFGLATLYDFGNAKLGFNWTNTKFLGLDKS
                     ATGATNWGGVSTGHVSFNTFEILGTYNFTASLVGGLFYNYTDGAISATRQEPRYHTVG
                     ATLDYFLSKRTDVYVLTTYMKAAGDAQYAALLPTVGASSSSSQAIFRIGVRHKF"
     gene            856..2403
                     /locus_tag="VGC69_16230"
     CDS             856..2403
                     /locus_tag="VGC69_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0296877.1"
                     /translation="MSNFDIDLNRRRLLSSAAAMAAGSFLPWEIASAQAAGDALSIAY
                     ISDVPVWDPTAITVPQVQSIFETVFDSPLRYSPATKLEARQIASWGWLDDKATRLGVK
                     LRDDITFHDGSKLTTEDLKWSFLDRPRADKKLAIGGMLNTLADVEIKSPTEAVLVYGK
                     PTPTAPIYLAFLGGYIVPKAYMQKVGDDFLNKPIGAGPYRLVEHQRGSAIILEAYDKY
                     WGGKPAIKNVTFQVTTDPSARVAAVESGRAGVAVQIPLREAQRLGKISGITSKIYPYT
                     EIYMLRMPNYVKPFDDPNVRRAMHLAIDTATLSKAFYGGIAKPISVTDTPGAPADVPG
                     FVFPYDPKQAVEALAKSGYGPDKPVQIPLLTTNGTFPSDYDMARAIAAMWKKVGIEAV
                     IQETTMAKVISEIQATKMPGALVYSWANSTGDPENYTGRILDPRLRFSAWKDDALIPR
                     IEQLMTEPDERKRIALYQDINKEASENSWAIPLLQAVTTIAYRNNVDVKTFESGYVLP
                     VEYKLKG"
     gene            complement(2485..3171)
                     /locus_tag="VGC69_16235"
     CDS             complement(2485..3171)
                     /locus_tag="VGC69_16235"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296878.1"
                     /translation="MTIERPAVAKISRLSQRSPTLMESVYQDILSRLQRGEIKQGERI
                     LDNDVAREFECTRTPVRHALLRLVNEGYLVGTTRGFVIPEWTAQDVRDIFEVRRLLEP
                     GAAASVALILSAEQLESMAASYRSCRRGAEKQDVPLITEANIKFRSIWLDTVPNPRLK
                     QEILRYSDHAQLVRRSTMPNPATMLAVADGMQLLLAGYSERDPAQVRDAMEAFVAHAE
                     RAYFQSIESE"
     gene            complement(3284..5155)
                     /locus_tag="VGC69_16240"
     CDS             complement(3284..5155)
                     /locus_tag="VGC69_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0296879.1"
                     /translation="MASTGTNFDPAMQDAPPVLRLDAVTLGARVGGRDVSALRDISLS
                     IRSGQVLGVVGESGAGKSMLTKLIAGLLPEGFGVRSGAMEFCGESLLAMSAAQRRELL
                     GRRIAFVPQEPLTALDPVWTLGATFREHLSRLGVPARERDGMAIRYLEAVQLPTPAKL
                     LERYPHQLSGGQCQRVLIAMAFANQPALLIADEPTTALDVVTQARVMTLLGEMQRSQD
                     TAVLLITHDLRLAAQVCDQIAVMYAGDLVETGPAAEVLETPRHPYTRALKHATPDLRG
                     ERRRLPSLPRQMPGLTALPDLPGCRFAPRCALADAPCAAGRHWRVNASGHGVACSEHC
                     ESGADASDKVQAPPPGMAEPGSRPIVELREASLAYRSTTGFFKPEHHAFHAVKSLSLK
                     IYPGEFVGIVGESGSGKTSVARLVMGIESPTEGRLLIDGEDISYGNAEMLRRARERVQ
                     IIFQDPQSALNPRSQVERLLTQAMEAVGLPALGAAQRRERAESLHRDVGLPQDCLGKF
                     PSQLSGGQKQRVNIGRALGASPALIVADEIVSGLDVSVQAQILNLLLDLRAQRTIALL
                     LISHDLSVVRYLCHRVLVMQRGEVVEQGETETVFANPQHPYTRELIAAVPGNHAEAS"
     gene            complement(5159..5995)
                     /locus_tag="VGC69_16245"
     CDS             complement(5159..5995)
                     /locus_tag="VGC69_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296880.1"
                     /translation="MSRNFNLWIGAAIVLLLLACAVFAPWLAPYPPLDQDLLQMLAPP
                     AWMNGGDWAHPLGTDSLGRDVLSRLLFGSRVALFVAVMSALGAGLVGSILAITAGYFG
                     GRFDRFITYLVDLWMSFPPVVLSLVLMVALGVGVDKVILAVVLVDWTRFCRVVRAEVL
                     VVRQREYILAAQLMNLSHLRIMLREVLPAALPLIITLFSLEMGVAVTVEALLSFVGLS
                     VSADVTAWGGMIADARGNMYESLSGLLFPVLAIVVTVLGCNMLGDGLRVALDPRLRRR
                     GE"
     gene            complement(6001..7020)
                     /locus_tag="VGC69_16250"
     CDS             complement(6001..7020)
                     /locus_tag="VGC69_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0296881.1"
                     /translation="MPRLILTILLRRLGAAIPTLFFLSLIVFLVLRMIPADPLGSMLP
                     PNATPADAQALRKALGMDQPLPAQFLIWVENAVTGDLGNSISYHQPVRSLIASTLPST
                     IELCVSALLVSLLISIPGGVLAYVVAGKRSDVPVSLAVVLMQSVPAFLWALLLMILLG
                     VILPILPFSGRADDGYTSAGITGFYLLDFLITGQLGAWFSTIRHLVLPSVSLAFGFAP
                     LVIRVLRSSLIDQATESYVAVARLRGTSERRILWLHMLKNASLPALTMIGVQFGFLFG
                     SALLVEMIFSYPGIGNLMIQAVRSNDMVLIQGIAVVFCALMLLINAIVDTLYVLLNPR
                     LRKTA"
     gene            complement(7064..8473)
                     /locus_tag="VGC69_16255"
     CDS             complement(7064..8473)
                     /locus_tag="VGC69_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0296882.1"
                     /translation="MNTQALLHTLTAELGAGQVILREDLGTRRYTDWSGTGGLAPLAV
                     LRPRDTGEVSRAMAICHAARMPVVVQGGLTGLAGGANVRGDAVVLSLERMQRIEEIDA
                     VSGTVTVQAGAILQNVQEAAAGVGLMFPHDLGARGSCTIGGNLATNAGGNRVIKYGMM
                     REQVLGLEAVLADGAVICDMHKMIKNNSGYSLQQLLVGSEGTLAVITRAVLRLRQRPA
                     VVETAWCGLPDYAAVTRLLSYAQARLSAGVSAFEVMWPGYYDFVMRHIKTVRPPLPTR
                     HAVYVLMESAGSDAQRHGAEFQSMLEAMLEEGVVENAAIAQSQGDAQAFWAVRDAPAE
                     FPLLMPNMCAFDISFAIADIGQVVAECEQLLSTRWPGSTILIYGHLGDGNLHVLADVP
                     GIDAPTAQAVDALVYELTRKWHGSVSAEHGIGDKKTAYLGHTRDPGALAAMHRIKAGL
                     DPLNLMNPGKLFPDAASRP"
     gene            complement(8473..9168)
                     /locus_tag="VGC69_16260"
     CDS             complement(8473..9168)
                     /locus_tag="VGC69_16260"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0296883.1"
                     /translation="MWFSLGSPTLVEMALAGEPDLIVLDLQHGLWERRTLEYAVGLAR
                     RSVPVIARLADHDAHSVSEALDAGVASILAPFVETAEQARALVSYAHFPPAGIRSAGG
                     VRALATGMGIALEDSRQVKVGAMIETVAGVAAVESIAAVPGLDYLFIGTGDLALSCAA
                     AGAQDPAATVRTDSLRILAAARARGIACGIFTDGIEDAKRQREAGFDFVVLLNDISMA
                     RDGFAAAGSAWRK"
     gene            complement(9698..10474)
                     /locus_tag="VGC69_16265"
     CDS             complement(9698..10474)
                     /locus_tag="VGC69_16265"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY0296884.1"
                     /translation="MAAFNTERVLNVRHWNDTLFSFTTTRDSALRFHNGHFVMIGLEV
                     EGKPLMRAYSIASANYEENLEFFSIKVPDGPLTSRLQHLKEGDSILVSKKPVGTLVAD
                     DLKPGKHLYLFATGTGLAPFLSIIKDPDVYERYEKVVLLHGVRHVNELAYADFIEKEL
                     PNNEFFGDVVRDKLIYYPCVTREPFRHQGRITALVENGKLFEDIGLPPLDPAVDRAMI
                     CGSPQMLADIRAMLDTRGFQVSPGVGEPGDYVFERAFAEK"
     gene            complement(10660..11070)
                     /locus_tag="VGC69_16270"
     CDS             complement(10660..11070)
                     /locus_tag="VGC69_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEY0296885.1"
                     /translation="MAKIRIRSAGYTFVAETNPDAPQTVEAFTKLLPYTQKIIHVRWS
                     GEGCWIPLGEFQLGVDFENHTSHPSVGDILFYPGGYSETEIILAYGSCMFASKMGQLA
                     GNHFLTITEGRENLRKLGTKVLWEGAQDIVFEAA"
BASE COUNT         2027 a         3752 c         3683 g         1728 t
ORIGIN      
        1 accaatacgg ccagctcctg ctgggccggc aagatgaaat gatggccgac tacgtcggca
       61 tgtacaccgc cgtccccctg ctgggcggca tcgaaacgcc ccaccccgcc gacctggaca
      121 acaccggcat cgacttccgc atcaacagcg cgatccgata caactcgccg aacatcggcg
      181 gcttcacggg cagcgccctg tacagcggcg atagcacgac cggcaccttc ggccagtcct
      241 cgaccaagtc cttcggcctg aactacaaca acggcggcct gaacatagcg ggggcctaca
      301 cgtccgtcgg caacccctcc agcctgaccg acgccttgtt cacctcgccg agcaacccga
      361 tcgcgggcta cggctactcc cagcgctacc aggccttcgg cctcgccacg ctgtacgact
      421 tcggcaacgc caagctgggc ttcaactgga ccaacaccaa gttcctgggc ctggacaaga
      481 gcgccaccgg cgcgaccaac tggggcggcg tcagcacggg ccacgtgtcc ttcaacacgt
      541 ttgaaatcct gggcacttac aacttcaccg ccagcctggt cggcggcctg ttctacaact
      601 acaccgacgg cgccatctcg gccactcgcc aggagcctcg ctaccacacg gtcggcgcca
      661 cgctcgacta cttcctgtcc aagcgcaccg acgtgtacgt gctcaccacg tacatgaagg
      721 ccgcgggcga cgcgcagtac gccgcgctgc tgcctaccgt cggcgcgtcc agcagcagtt
      781 cgcaagccat cttccgcatc ggcgtgcgcc acaagttctg atacggaaag cccacagcca
      841 cacgaggaat acgctatgtc caacttcgac atcgatctga accgccgacg cctgctgtcc
      901 agcgcggcgg cgatggcggc cggctcgttc ctgccctggg aaatcgcctc ggcgcaagcc
      961 gccggcgacg cgctgagcat cgcctacatc agcgacgtac ccgtatggga tccgaccgcc
     1021 atcacggtcc cgcaggttca atccattttc gaaacggtct tcgattcgcc cctgcgctac
     1081 tcgccggcca ccaaactgga agcccggcag atcgcctcgt ggggctggct cgacgacaag
     1141 gccacccgcc tgggcgtcaa gctgcgtgac gacatcacct tccacgacgg gtccaagctc
     1201 accaccgaag acctcaagtg gagcttcctc gaccggccgc gggccgacaa gaaactggcg
     1261 atcggcggca tgctcaacac gctcgccgac gtcgagatca agtcgcccac cgaggccgtg
     1321 ctggtctacg gcaagcccac gcccaccgcc ccgatctacc tggccttcct gggcgggtac
     1381 atcgtgccca aggcctatat gcagaaggtc ggcgacgact tcctcaacaa acccatcggc
     1441 gccggcccct accgcctggt cgagcaccag cgcggctcgg ccatcatcct cgaggcctac
     1501 gacaagtact ggggcggcaa gcccgccatc aagaacgtga ccttccaggt cacgaccgac
     1561 ccgtcggcgc gcgtggcggc ggtggaatcg ggccgcgccg gcgtggcggt gcagatcccc
     1621 ctgcgcgagg cgcaacgcct gggcaagatc tcgggcatca ccagcaagat ctatccgtac
     1681 accgagatct acatgctgcg catgcccaac tacgtcaagc ccttcgacga cccgaacgtg
     1741 cgccgggcta tgcacctggc catcgacacg gccacgctgt ccaaggcctt ctacggcggc
     1801 atcgccaagc cgatctcggt gaccgacacc ccgggcgcgc ccgccgacgt gccgggcttc
     1861 gtgttcccct acgaccccaa gcaggccgtc gaggccttgg ccaagtccgg ctacgggccg
     1921 gacaagccgg tgcagattcc cttgctgacg accaacggca ccttccccag cgactacgac
     1981 atggcgcgcg ccatcgccgc catgtggaag aaggtcggca tcgaggccgt catccaggaa
     2041 accaccatgg ccaaggtcat cagcgagatc caggcgacca agatgcccgg cgcgctggtg
     2101 tacagctggg ccaactcgac cggcgatccg gagaactaca ccggccgcat cctcgacccg
     2161 cgcctgcgct tctcggcctg gaaggacgac gcgctgatcc cgcgcatcga gcagttgatg
     2221 accgagcccg acgagcgcaa gcgcatcgcg ctctaccagg acatcaacaa ggaagcctcg
     2281 gaaaactcct gggccattcc gctgctgcaa gccgtgacga ccatcgccta ccgcaacaac
     2341 gtggacgtca agaccttcga gagcggctat gtcctgccgg tggagtacaa gctcaagggc
     2401 tgagccgcgg gccaggcgcc gcgcgccgat cggacctgcg gcgcccttgg aaaacgatgc
     2461 ccccgcagac ggcgggggca tcgttcattc ggactcgatg ctctggaagt aggcgcgctc
     2521 ggcgtgggcg acgaaggctt ccatggcgtc gcgcacctgc gccggatcgc gctcgctgta
     2581 gcccgccagc agcagctgca tgccgtcggc gacggcgagc atggtggccg ggttgggcat
     2641 ggtgctgcgg cgcaccagct gcgcgtggtc ggaatagcgc aggatttcct gcttcagccg
     2701 gggattgggc acggtgtcga gccagatcga gcggaacttg atgttggctt cggtaatgag
     2761 ggggacgtcc tgcttttcgg cgccgcggcg gcagctgcgg taggacgcgg ccatcgattc
     2821 gagctgctcc gccgacagga tgagggctac gcttgccgcc gcgccgggtt cgagcagccg
     2881 gcgcacttcg aaaatgtccc ggacgtcctg ggccgtccat tcggggatga cgaaaccgcg
     2941 cgtggtgccg accagatagc cttcgttgac caggcgcagc aaggcatgcc gcaccggcgt
     3001 gcgcgtgcat tcgaattcgc gggctacgtc gttgtcgagt atgcgctcgc cctgtttgat
     3061 ctccccgcgt tgcagacgac tgaggatgtc ttggtagacg gactccatga gcgtgggaga
     3121 tcgctgggaa agacgggata tcttggccac agcaggtcgc tcgattgtca tatgctccga
     3181 aagcccctgg atgggatgcg cctcaatgtt tcgattgtac tacgcgcact ttttgcaaaa
     3241 tacgcgcggc gggcccgcgc ggcgggcatc aggccgttgt cagtcaagac gcctcggcat
     3301 ggttgccggg cacggccgcg atcagttcgc gggtataggg atgctgcggg ttggcgaaga
     3361 cggtttcggt ctcgccctgc tccacgacct cgccgcgctg catcaccagc acgcggtggc
     3421 aaagatagcg gaccaccgac aggtcatgcg agatcagcag caaggcgatg gtgcgctgcg
     3481 cccgcaggtc cagcagcaag ttgaggatct gcgcctgcac cgagacgtcg agcccggaga
     3541 cgatctcgtc ggccacgatc agcgcgggcg aggcgcccag ggcccggccg atgttcacgc
     3601 gctgcttctg cccgcccgac agctggctgg ggaacttgcc caggcagtcc tgcggcaggc
     3661 cgacatcgcg gtgcagggac tcggcgcgtt cgcggcgctg cgccgcgccg agcgcgggca
     3721 ggcccacggc ctccatggcc tgggtcagca gccgctcgac ctggctgcgc ggattgagcg
     3781 cggactgggg gtcctggaaa atgatctgca cgcgctcgcg ggcgcgccgc agcatctcgg
     3841 cgttgccgta ggagatgtct tcgccgtcga tcagcaggcg cccttcggtg ggcgactcga
     3901 tgcccatcac cagccgggcg acggacgtct tgccgctgcc gctctcgccc acgatgccca
     3961 cgaactcgcc gggatagatc ttcagcgaca ggctcttgac cgcatggaag gcatgatgct
     4021 cgggcttgaa aaagcccgtc gtggaacgat aggcgagcga ggcttcgcgc aattcgacga
     4081 tgggccgcga gccgggctcg gccatgcccg gcggcggcgc ctgcaccttg tcgctggcgt
     4141 cggcgccgga ctcgcaatgt tcgctgcagg ccacgccatg gccgctggcg ttcacgcgcc
     4201 agtgcctgcc ggcggcgcat ggcgcgtcgg ccagcgcgca gcgcggcgcg aagcggcagc
     4261 cgggcaggtc cggcaatgcc gtgagtccgg gcatctgcct gggcagcgaa ggcaggcggc
     4321 ggcgctcgcc ccgcaggtcg ggggtggcgt gcttgagggc tcgggtatag gggtggcgcg
     4381 gcgtttccag cacctcggct gccggccccg tctcgaccag gtcgcccgcg tacatgaccg
     4441 cgatctggtc gcagacctgg gccgccagcc ggagatcgtg cgtgatcagc agcaccgccg
     4501 tgtcctggct gcgctgcatc tcgcccagca gcgtcatcac gcgcgcctgg gtgacgacgt
     4561 cgagcgcggt ggtcggctcg tcggcaatca gcagggccgg ctggttggcg aaggccatgg
     4621 cgatcagcac ccgctggcac tgccccccgg acaactggtg cggatagcgc tccagcagct
     4681 tggccggcgt gggcagctgc accgcctcca ggtagcggat cgccatgccg tcccgttcac
     4741 gggccggcac gcccaggcgc gacagatgct cgcggaacgt cgcgcccagg gtccagaccg
     4801 gatccagggc ggtcaggggc tcttgcggca cgaaggcgat gcgccggccc agcagctcgc
     4861 ggcgctgggc cgcggacatg gccagcagcg actcgccgca gaattccatg gcgccgctgc
     4921 gcacgccgaa gccttccggc agcaggcccg cgatcagttt ggtcagcatg ctcttgccgg
     4981 cgcccgactc gcccaccacg ccgagcacct ggccgctgcg gatggacagg gaaatatccc
     5041 gcagggccga cacgtcgcgg ccgcccacgc gggcgccgag cgtcacggcg tccagcctga
     5101 gtacgggcgg cgcgtcctgc atggccggat cgaagttcgt gcctgtgctt gccatggatc
     5161 attcccccct gcggcgcagg cgcgggtcga gcgccacgcg caatccgtcg cccagcatat
     5221 tgcagcccag caccgtcacg acgatggcca gcaccggaaa cagcagcccc gagagcgatt
     5281 catacatatt gccgcgcgcg tcggcgatca tgccgcccca cgccgtgacg tcggccgaca
     5341 cgctcaggcc gacgaagctc agcagcgcct cgaccgtcac ggccacgccc atttccagcg
     5401 agaacagggt gatgatcagg ggcaaggcgg ccggcaatac ctcgcgcagc atgatgcgca
     5461 ggtgggacag gttcatgagc tgcgccgcca ggatgtattc gcgctgccgc acgaccagca
     5521 cttcggcgcg cacgacccgg cagaaccgcg tccagtccac cagcacgacg gccaggatca
     5581 ccttgtccac gcccacgccc agcgccacca tcagcaccag gctgagcacg acgggcggga
     5641 aggacatcca caggtcgacc agatacgtga tgaagcggtc gaagcgtccg ccgaaatacc
     5701 ccgccgtgat cgccaggatg ctgcccacca gccccgcgcc cagggccgac atcaccgcga
     5761 caaacagcgc cacccggctg ccgaacagca gccgcgacag cacgtcgcgg cccaggctgt
     5821 cggtgcccag cgggtgagcc cagtccccgc cgttcatcca ggcgggcggc gccagcatct
     5881 gcaggaggtc ctggtccagc ggcgggtaag gcgccagcca cggcgcgaac accgcgcagg
     5941 ccagcaacag caggacgatg gccgccccga tccacaaatt gaaattgcgc gacatggtgc
     6001 tcaagccgtt tttctcaggc gcgggttcaa caggacatac agcgtgtcca cgatggcgtt
     6061 gatcagcagc atcagggcgc agaacacgac ggcgatcccc tggatcagca ccatgtcgtt
     6121 gctgcgcacg gcctggatca tgagattgcc gatgccgggg taggagaaga tcatctccac
     6181 cagcagcgcg ctgccgaaca ggaagccgaa ctgcacgcct atcatggtca gggccggcag
     6241 cgaggcgttc ttgagcatgt gcagccacag gatgcggcgc tcggacgtgc cgcgcagacg
     6301 cgcgacggcc acgtaggatt cggtggcctg gtctatcagg ctggagcgca gcacgcggat
     6361 gaccagcggc gcgaagccga acgccaggga gacggacggc aggacgagat gccgtatcgt
     6421 gctgaaccag gcccccagct gccccgtgat caggaagtcc agcaggtaga agccggtgat
     6481 gcccgcgctg gtgtagccat cgtcggcgcg gccggaaaac ggcaggatgg gcaggatcac
     6541 gcccagcagg atcatcagca gcagcgccca cagaaaggcc ggcacggact gcatcagcac
     6601 cacggccagg cttaccggca cgtcgctgcg cttgcccgcc accacatagg ccaggacgcc
     6661 gcccgggatg ctgatcagca gcgacaccag cagcgcgctc acgcacagct cgatggtcga
     6721 cggcagggtc gacgcgatca gcgacctcac cggctggtgg tacgagatcg aattgcccag
     6781 gtcgcccgtc acggcgttct cgacccagat gaggaactgc gcgggaagcg gctggtccat
     6841 ccccagggcc ttgcgcagcg cctgcgcgtc ggcgggcgtg gcgttgggcg gcagcatcga
     6901 ccccaggggg tcggcgggga tcatgcgcag caccaggaag acgatcagcg acaggaagaa
     6961 cagcgtggga atcgccgcgc cgagccggcg cagcaaaatg gtcaatatca ggcgcggcat
     7021 ggcgatggaa ctctgcagcg gagcgaacgc agaaggaagt cggtcatgga cgggaggcgg
     7081 cgtccgggaa cagcttgccc gggttcatca ggttcagggg atcgagcccc gccttgatcc
     7141 ggtgcatcgc ggccagcgcg cccgggtcgc gggtatggcc gaggtaggcg gtcttcttgt
     7201 cgccgatgcc gtgctcggcc gagaccgagc cgtgccactt gcgcgtcagc tcgtagacca
     7261 gggcgtccac ggcctgcgcg gtcggcgcgt cgatgcccgg gacgtcggcc agcacgtgca
     7321 ggttgccgtc gcccagatgg ccgtagatca ggatggtcga gcccggccag cgcgtgctca
     7381 gcaactgctc gcactcggcc acgacctggc cgatgtcggc gatggcgaag ctgatgtcga
     7441 aggcgcacat attgggcatc agcaggggaa actcggccgg cgcatcgcgc acggcccaga
     7501 aggcctgggc gtcgccctgc gactgggcga tcgccgcgtt ctccacgacg ccctcttcca
     7561 gcatggcctc gagcatggac tggaattcgg cgccgtgccg ctgcgcgtcc gacccggcgc
     7621 tttccatcag gacatagacg gcgtggcgcg tgggaagcgg cgggcgcacg gtcttgatgt
     7681 ggcgcatgac gaaatcgtag tagccgggcc acatgacctc gaacgccgaa acgcccgccg
     7741 agaggcgcgc ctgcgcgtac gacagcaggc gcgtcaccgc ggcgtaatcg ggcaggccgc
     7801 accaggccgt ctcgaccacg gccgggcgct gccgcaggcg cagcacggcc cgggtgatca
     7861 cggccagcgt gccttcgctg ccgaccagca gttgctgcag gctgtagccg ctgttgttct
     7921 tgatcatctt gtgcatgtcg cagatcacgg cgccatcggc cagcacggcc tccaggccca
     7981 gcacctgctc gcgcatcatg ccgtacttga tgacgcggtt gccgccggcg ttggtggcga
     8041 ggttgccgcc gatggtgcag ctgccgcggg cgccgagatc gtgcggaaac atcagcccga
     8101 cgccggcggc ggcctcctgc acgttctgca ggatggcacc ggcctgcacg gtgaccgtgc
     8161 cggagaccgc gtcgatttcc tcgatgcgct gcatgcgctc caggctcagc accacggcat
     8221 cgccgcgcac gttggccccg cccgccaggc cggtcaggcc gccctgcacc accacgggca
     8281 tgcgcgccgc gtggcagatc gccatggcgc gcgacacctc gccggtgtcg cgcggacgca
     8341 gcacggccaa aggcgccagg ccgccggtgc cgctccagtc cgtatagcgg cgcgtgccca
     8401 ggtcttcgcg caaaatgacc tggccggcgc ccagctcggc cgtcagcgta tgcagcaatg
     8461 cttgagtatt catcacttac gccaggcgga gcccgccgca gcgaacccgt cccgggccat
     8521 ggatatatcg ttgagcagca ccacgaaatc gaagccggcc tcgcgctgcc gcttcgcgtc
     8581 ctcgatcccg tcggtgaaga tgccgcaggc gatgccgcgt gcccgggcgg ccgccaggat
     8641 gcgcaggctg tcggtccgca ccgtggcggc cggatcctgc gccccggcgg cggcgcacga
     8701 cagggccagg tcgcccgtgc cgatgaacag gtagtccagc cccggcacgg cggcgatgga
     8761 ttcgacggcg gcgacgcccg ccacggtttc gatcatggcg cccaccttga cctgccggct
     8821 gtcctcgagg gcaatgccca tgcccgtggc caacgcccgg acgccgccgg ccgagcgtat
     8881 ccccgccggg ggaaagtgcg cgtaggaaac aagggcgcgc gcctgctcgg ccgtctcgac
     8941 gaacggcgcc agaatggacg ccacgccggc gtccagcgcc tcggacaccg aatgcgcgtc
     9001 gtggtcggcc agccgggcaa tgacgggaac cgagcgccgg gccaggccca ccgcatattc
     9061 cagcgtgcgc ctttcccaga gcccgtgctg cagatccagg acgatcaggt ccggttcgcc
     9121 ggccagcgcc atctcgacca gcgtcgggct gcccagcgag aaccacatgc aacctagtgc
     9181 ctggcgctgc ccagcctggt cggcctgggg aaactgcaaa gggcgcggaa tgaatcgaag
     9241 acacgaggag gacggcaaaa tcagttcacc acaaagggtg ccttataaaa ttcaggattc
     9301 aaattctagg aaagccaccg gtttgcatct acccggagca acccgaagtg gccacgtcgc
     9361 atgcgctaac ggaaggctga caggcacggt cgacatggat cagagcctcc ggcgcggagc
     9421 tgcgcgcgat accgggcatc tcacaaaata gcccactatt ttagaattca gaatactatt
     9481 taaaaatagg ggcgtcaacg aagacttccc cggatccgcc ctgcccgccc caccctgcgg
     9541 cccaggcgat tacggttgcc gcgccatggg gcggacacat agccgagcct cgaaaaaagc
     9601 gggaagcaac ccaagacaaa acggcctgag aggcaaaccc cccaggccgt ttcaatgccg
     9661 ctcgatcaga gcggcttcgc tatcgcggac gcagtattta cttctcggcg aacgcccgtt
     9721 cgaacacata gtcgccgggc tcgcccacgc cgggggagac ctggaagccg cgggtgtcca
     9781 gcatggcgcg gatgtcggcc agcatctggg ggctgccgca gatcatggcg cggtcgacgg
     9841 cggggtcgag gggcggcagg ccgatgtcct cgaagagctt gccgttttcg accagagcgg
     9901 tgatgcgccc ctggtggcgg aaaggctcgc gggtgacgca ggggtagtag atgagcttgt
     9961 cgcgcaccac gtcgccgaag aactcgttgt tgggtagttc tttttcgatg aaatcggcgt
    10021 aggccagttc gttgacgtgg cgcacgccat gcagcagcac gactttttcg tagcgttcgt
    10081 agacgtccgg gtccttgatg atgctcagaa acggggcgag gcccgttccg gtggcgaaca
    10141 ggtacaggtg cttgccgggc ttgaggtcgt cggccaccag ggtgcccacg ggcttcttgc
    10201 tgaccaggat ggaatcgcct tctttgaggt gctgcaggcg cgaggtgagc gggccgtcgg
    10261 gcaccttgat gctgaagaac tcgaggtttt cctcgtagtt ggcgctggcg atgctgtagg
    10321 cgcgcatcag cggcttgccc tcgacttcca ggccgatcat gacgaagtgg ccgttgtgaa
    10381 agcgcagtgc ggaatcgcgg gtggtggtga acgagaaaag ggtgtcgttc cagtggcgca
    10441 cgttgagtac gcgctcggta ttgaaagctg ccatgacgag gagaagtgcc tgaaaatctg
    10501 ggcgtaaaac gttgaaagta cggtaatttt accgcactca gttgataatc gctctcaatt
    10561 gttgcgccat atccttacta cttttagata ttaagtatta aaaattagtc gcatggggaa
    10621 atgatgcagc gcaggacaag gcgcaagcgc cgccaggctt caggccgcct cgaagacgat
    10681 gtcctgcgcg ccttcccaga gcaccttggt gcccagcttg cgcaggtttt cgcgcccttc
    10741 ggtaatggtc aggaaatggt tgccggccaa ctggcccatc ttgctggcga acatgcagct
    10801 gccataggcc aggatgatct cggtttcgct gtagccgccg ggatagaaca ggatgtcgcc
    10861 caccgagggg tggctggtgt ggttctcgaa gtccacgccc agctggaact cgcccagcgg
    10921 aatccagcac ccctccccgc tccagcgcac atgaatgatc ttctgcgtgt aaggcagcag
    10981 cttcgtgaag gcctcgacgg tttgcggggc gtcggggttg gtttcggcga cgaaggtgta
    11041 gccggcggag cggatgcgga tcttggccat ggcgtaaggg gcgactcaat gtttatcggg
    11101 taaggggatg aagtctaccg tgtcgttggg cacgacttgg tcgaaacggt tgcgctgcca
    11161 gtcttccttg gcctgttcga tgcgttcccg
//
LOCUS       DASWPO010000312         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1413991, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000312 DASWPO010000000
VERSION     DASWPO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413991"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VGC69_16275"
     CDS             <1..1031
                     /locus_tag="VGC69_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0296886.1"
                     /translation="LVALAAVSSSVCAAPAPLVRDIAPSLYEVVYSPHSDAVFVASAR
                     NPDGEGGVVYKLDPATLAVRARIAVPLKPFSLALDEKTDTLYVGHTVDAAVSAIDAAT
                     GTLKGTLRHGEVDAEGKPVHVRELAVDARAGLLFVGGVSDGGFLWVIDTATFKTRKIL
                     RGGDLPSIGLTPDPAHQRLYVSGTAAYAVIDTRTLATIGTQRIPEKRDDKDGKRRFLV
                     NTALDADGKRLFANQLNNGEGTLVFDTASGKLLQTIATGDMPLGIRFNPARNEVYVAS
                     RGSGTFSVIDADSYAIKRSIALPTHPNTIALSKDGKTAWVTVKQASAGRDAPPVDEQV
                     ARIDLATP"
     gene            1105..1839
                     /gene="exbB"
                     /locus_tag="VGC69_16280"
     CDS             1105..1839
                     /gene="exbB"
                     /locus_tag="VGC69_16280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HEY0296887.1"
                     /translation="MHIETTLAFAALPHDLTAWAMYRNATTVVQAVMVGLALASIATW
                     TVLVAKAWELRRQRRELRAAHAVLSQSPRLGEAAAQDALQAGYAQRLIDAAGTELRLS
                     NGLEDKAGVKERVASRLERLELGQARQLRRGTGVLATIGAVAPFVGLFGTVWGIMNSF
                     IGIAQSNTTNLAVVAPGIAEALLATALGLVAAIPAVVIYNHLSRRLAELRDLLGDVSA
                     GVQQLVSRDLDRSGAPNGASLLHLAR"
     gene            1849..2274
                     /gene="exbD"
                     /locus_tag="VGC69_16285"
     CDS             1849..2274
                     /gene="exbD"
                     /locus_tag="VGC69_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HEY0296888.1"
                     /translation="MAITRGSRADDGLDEQHEINVTPFIDVMLVLLIIFMVAAPLATV
                     DVPVDLPASTAQPAPKDHDSIYLTVQPDLSLRLNQDPVTAQALGSRLDALTQGHHDER
                     IFLRADKGVPYGELMSTMNALRDAGYLKVALVGLEHGQG"
     gene            2271..3011
                     /locus_tag="VGC69_16290"
     CDS             2271..3011
                     /locus_tag="VGC69_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0296889.1"
                     /translation="MSASLPNMAPHEGLRWGGSLGLVLLAHALAVAAVLWWGARVRLV
                     VADAPLEAVLVDLAPEPVTPPAPPQEVPQEVPPGPPQRQQQRSPPQPKPPPPPDVVPE
                     VVEPTPPAPSPQPPATEDIAQSLAPPAAKSVEARTAAAQTAAGRSDGAQATWQGKLLG
                     HLQRHRRYPQQAQWHRQEGVVYVRFEVDRGGNVSGIRLGRSSGLPALDEEALATVKRA
                     SPVPAPADEVEGDPVEVMVPVEFFLRRR"
     gene            complement(3021..3506)
                     /locus_tag="VGC69_16295"
     CDS             complement(3021..3506)
                     /locus_tag="VGC69_16295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0296890.1"
                     /translation="MSGPAGFRDVGAADLPGLLALNNAHAAALSWQTPTQFSQLIDMA
                     CFAQTCGEADALLIALDQDAAYDNQNFAWLSARFERFVYIDRVVVAEHMQGRGLAGAL
                     YAALALQARSQQQPRLVCEINLDPPNPGSVAFHRRLGFEQVGQADLPNGKRVGYFACT
                     L"
     gene            complement(3503..>4376)
                     /gene="ddlA"
                     /locus_tag="VGC69_16300"
     CDS             complement(3503..>4376)
                     /gene="ddlA"
                     /locus_tag="VGC69_16300"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY0296891.1"
                     /translation="IPGSPRQPLRPVDPATALGQIDVVFNIVHGTLGEDGSLQGLLRM
                     ANLPFVGSGVLGSAVAMDKDVAKRLLRDAGLAVAPFVCLTRRSAGLVTHAELVQRLGP
                     TLFVKPANQGSSVGVSRVTDAAGFDAALAQAFLYDHKVLVEAGIAGREIECAVLGNDT
                     PQASVCGEVVVHDAFYAYDTKYIHEDGAQIVVPADIDGDTQLRIQQVALRAFQALECA
                     GLARVDVFLTPAGEIVVNEINTLPGFTRISMYPKLWQASGMTYPKLVTRLIELALERH
                     ARDSNLRSSVALDA"
BASE COUNT          667 a         1550 c         1523 g          636 t
ORIGIN      
        1 tgctggttgc actggccgcg gtctcctcgt ccgtctgcgc cgcgcccgcg ccgctggtgc
       61 gcgacatcgc ccccagcctg tacgaagtcg tctacagccc gcacagcgac gcggtcttcg
      121 tcgccagcgc ccgcaacccc gatggcgagg gcggcgtggt gtacaagctg gacccggcca
      181 cgctggccgt gcgtgcgcgc attgcggtgc cgctcaagcc cttctcactg gcgctggacg
      241 aaaagaccga caccttgtac gtcggccata ccgtcgatgc cgcggtcagt gccatcgacg
      301 cggccaccgg cacgctcaag ggcacgctgc gccacggcga ggtggatgcc gagggcaaac
      361 cggtgcacgt gcgcgagttg gcggtggatg cgcgcgccgg cctgctgttc gtcggtggcg
      421 tgagcgacgg cgggttcctg tgggtgatcg acacggccac cttcaagacc cgcaagatcc
      481 tgcgcggtgg cgacctgccc agcatcggcc tgacccccga cccggcccac cagcgcctgt
      541 acgtcagcgg caccgccgcc tatgcggtga tcgacacccg cacgctggcg acgatcggca
      601 cgcagcgcat cccggagaag cgcgacgaca aggacggcaa gcgccgtttc ctggtcaaca
      661 ccgcgctgga cgccgacggc aagcgcctgt tcgccaacca gctcaacaac ggcgaaggca
      721 cgctggtgtt cgacaccgcc tcgggcaagc tgctgcagac catcgccacc ggtgacatgc
      781 cgctgggcat ccgcttcaac ccggcgcgca acgaggtcta tgtcgccagc cgcggcagcg
      841 gcaccttcag cgtgatcgat gccgacagct atgcaatcaa gcgcagcatc gccctgccga
      901 cccatcccaa caccatcgcg ctgtccaagg acggcaagac cgcctgggtg accgtcaagc
      961 aggcctccgc cgggcgcgac gcaccgccgg tggacgagca ggtggcgcgg atcgacctgg
     1021 ccacgccctg acctgcatgc cgccccggag cggcgtgcct gttggcgtac gtcggccgta
     1081 tcccttcgca tccagagaca ccacatgcac atcgagacga ccctggcctt cgcggccctt
     1141 ccccacgacc tgacggcctg ggccatgtac cgcaacgcca ccacggtggt gcaggcggtg
     1201 atggtcggcc tggcgctggc ctccatcgcg acctggacgg tgctggtcgc caaggcctgg
     1261 gaactgcgcc gccagcggcg tgaactgcgt gccgcccatg ccgtcctgtc ccagtcgccc
     1321 cggctgggcg aagccgccgc gcaggacgca ctgcaggccg gctatgcgca gcgcttgatc
     1381 gacgcggcgg ggaccgaact gcgcctgtcc aacggcctgg aggacaaggc cggggtgaag
     1441 gagcgcgtgg cctcacgcct ggagcgcctg gaactgggcc aggccaggca gctgcgccgc
     1501 ggcaccggcg tgctggccac catcggcgcg gtggcgccgt tcgtgggcct gttcggcacc
     1561 gtgtggggca tcatgaacag cttcatcggc atcgcccagt ccaacaccac caacctggcg
     1621 gtggtggcac cgggcatcgc cgaggcgttg ctggcgaccg cgctcggact ggtcgcggcg
     1681 atcccggcgg tggtgatcta caaccatctc agccgcaggc tggcggaact gcgcgacctg
     1741 ctgggcgatg tctcggccgg tgtgcagcag ctggtctcgc gcgaccttga ccgcagcggc
     1801 gcgccgaacg gcgccagcct gctgcatttg gcgcgttgag gccgggccat ggcgatcacc
     1861 cgaggcagcc gcgccgatga cggcctggac gagcagcacg agatcaacgt cacccccttc
     1921 atcgacgtga tgctggtgct gctgatcatc ttcatggtgg ccgcgccgct ggccaccgtc
     1981 gatgtcccgg tcgacctgcc ggccagcact gcccagccgg cgcccaagga ccatgactca
     2041 atctatctca ccgtgcagcc ggacctgtcg ctgcggctga atcaggatcc ggtcaccgcc
     2101 caggcgctgg gctcgcgcct ggatgccttg actcagggtc atcacgacga gcgcatcttc
     2161 ctgcgcgccg acaagggcgt gccctatggc gagctgatgt ccaccatgaa cgcgttgcgc
     2221 gatgcgggct atctcaaggt cgcgctggtc ggcctggagc atggtcaggg atgagcgcca
     2281 gcctgccgaa catggcgccg cacgaaggcc tgcgctgggg tggcagcctg ggcttggtgc
     2341 tgctggcgca tgcgctggcc gtggccgcgg tgttgtggtg gggcgcgcgg gtgcggcttg
     2401 tggtggccga tgccccgctg gaggcggtgc tggtcgacct ggccccggag ccggtgacgc
     2461 cgccggctcc gccgcaggag gtgccgcagg aggtgccgcc cggcccgccg cagcgccagc
     2521 agcagcgcag cccgccgcag cccaagccgc caccgccgcc ggatgtcgtc ccggaggtgg
     2581 tcgagcccac gcccccggcg ccaagcccgc agccgccggc caccgaggac atcgcccaga
     2641 gcctggcgcc gcccgcggcc aagtcggtcg aggcgcgcac cgcggcggcc cagaccgcgg
     2701 ccgggcgcag cgacggcgcg caggcgacct ggcagggcaa gctgctgggc cacctgcagc
     2761 ggcatcgccg ctatccgcag caggcccagt ggcaccgcca ggagggcgtg gtctacgtgc
     2821 gcttcgaggt ggatcgaggg ggcaacgtgt ccggcatccg cctgggccgc agcagcgggc
     2881 tgcccgcgct ggacgaggag gcgctggcga cggtcaagcg cgccagccca gtgcccgcgc
     2941 cggccgacga ggtcgagggc gacccggtcg aagtgatggt gccggtggag ttcttcctgc
     3001 gccgtcgctg agccggatcg ctacagcgtg caggcgaagt agcccacgcg cttgccattg
     3061 ggcaggtcgg cctggccgac ctgctcgaag cccaggcgtc gatggaatgc gaccgaaccc
     3121 ggattgggcg ggtcaaggtt gatttcgcac accaggcgcg gctgctgttg cgagcgtgcc
     3181 tgcagtgcca gcgccgcgta cagcgcgccg gccagtcctc ggccctgcat gtgctcggcc
     3241 accaccacgc ggtcgatgta gacgaagcgc tcgaagcgtg ccgatagcca ggcgaagttc
     3301 tggttgtcgt atgcggcgtc ctggtccagc gcgatcagca gggcgtcggc ctcgccacag
     3361 gtctgcgcga agcaggccat gtcgatgagt tgcgagaact gcgtcggcgt ctgccacgac
     3421 aaggccgcgg cgtgtgcgtt gttgagcgcc agcagcccgg gcaggtcggc cgcgccgacg
     3481 tcacggaagc cggctggccc gctcacgcgt ccagcgccac gctgctgcgc agattgctgt
     3541 cgcgcgcatg gcgctccagc gcaagttcga tcaggcgggt gaccagcttg ggataagtca
     3601 tgccactggc ctgccacagc ttgggataca tgctgatgcg ggtgaagccg ggcagggtgt
     3661 tgatctcgtt gaccacgatc tcgccggccg gcgtgaggaa gacatcgacc cgtgccagcc
     3721 ctgcgcactc cagcgcctgg aaggcgcgca acgccacctg ctggatgcgc agttgcgtat
     3781 cgccgtcgat gtcggccggg accacgatct gcgcaccgtc ctcatggatg tacttggtgt
     3841 cgtaggcgta gaaggcatcg tgcacgacga cttcgccaca cacgctggcc tgcggcgtgt
     3901 cgttgcccag cacggcgcat tcgatctcgc gaccggcgat gcccgcttcc accagcacct
     3961 tgtggtcgta caggaatgcc tgcgccagcg ccgcgtcgaa acccgccgcg tcggtcaccc
     4021 ggctcacgcc gaccgaggag ccctgattgg ccggtttgac gaacagcgtc ggccccaggc
     4081 gctggaccag ttcggcatgg gtcaccaagc ctgcgctgcg gcgggtcagg cagacaaagg
     4141 gcgccaccgc caggccggca tcgcgcagca ggcgcttggc cacgtccttg tccatcgcca
     4201 ccgccgagcc cagcacgccc gagcccacga acggcagatt ggccatgcgc agcaggccct
     4261 gcagcgaacc gtcttcgccc agggtgccgt ggacgatgtt gaacaccacg tcgatctgtc
     4321 ccagcgcggt ggccggatcg accgggcgca gcggctgccg tgggctgcca gggatc
//
LOCUS       DASWPO010000313        17375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1861580, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000313 DASWPO010000000
VERSION     DASWPO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 17375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17375
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861580"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..569)
                     /locus_tag="VGC69_16305"
     CDS             complement(78..569)
                     /locus_tag="VGC69_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY0296892.1"
                     /translation="MNKPFSPRPRSKAGRPQETALSWLGHDTRGAGVLATAQRHLQIQ
                     QALAALLPPGLGEVCAVIKLDNQRLELAVPGSAYAAKLRQMAPSLAQALATRGWLLNE
                     IAVRIQAGMPRPGSRAARLPKTVQPLGADALQAFDDLGKSVRPGPLADAIARLLAHHK
                     KGS"
     gene            687..3416
                     /gene="secA"
                     /locus_tag="VGC69_16310"
     CDS             687..3416
                     /gene="secA"
                     /locus_tag="VGC69_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY0296893.1"
                     /translation="MISLLKKLIGSRNDRLLKQYRKLVGQINALEPQMAALSDEALAA
                     KTQEYRSRIEQGATLDDLLPEAFATVREAGKRVFGMRHFDVQLLGGIALHNGKIAEMR
                     TGEGKTLMATLPVYLNALAGKGVHVVTVNDYLARRDAETMGQLYRFLGLSTGVVVPQQ
                     ENEEKIASYRADITYGTNNEFGFDYLRDNMEYRVEDRRQRPLFYAIVDEVDSILIDEA
                     RTPLIISGQAEDHTDLYVRMNSVPPMLARMAAEPKPQEPEPEGDYWVDEKSQQVYLSE
                     AGHEHAEELLSQSGILPAGESLYDPRHIALMHHLMVALRAHTLFFRDQQYVVQDGEVV
                     IVDEFTGRLMVGRRWSDGLHQAVEAKEGVKIENENQTLASITFQNYFRMYEKLAGMTG
                     TADTEAYEFQEIYTLETVIIPTNKPMVRKDQNDQVFKTHQEKYNAILEDIRDCHARGQ
                     PVLVGTTSIENSELLSSLLNQAKLPHAVLNAKQHAREAEIVAEAGKPGHITIATNMAG
                     RGTDIVLGGSMDKQIALIRADESLSEADREARIQQVRADWKPANEQVKAAGGLRIIGT
                     ERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDPLMRIFAGDRVRSIMDRLKLPEGEP
                     IEAGMVTRSIETAQRKVEGRNFDIRKQLLEYDDVANDQRKVLYAQRNEVLEAGSISET
                     VASLTESVITDLFHAHVPPESVEDQWDVAGLQQVLANDWQLQLPLTDMLEEEPDLAEE
                     DLRERVLAAAREAYASKIALVGQESWSQFERSIMLQAIDSSWREHLSALDYLRQGIHL
                     RGYAQKNPKQEYKREAFELFSGMLDRIRNDVVRVLLTVRVQSAEQLEEAEGETEQMQN
                     LQFQHSDPDAELAAVAAPQAAAVPAGAVPRVGRNDPCPCGSGKKYKQCHGKLT"
     gene            3430..4152
                     /locus_tag="VGC69_16315"
     CDS             3430..4152
                     /locus_tag="VGC69_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0296894.1"
                     /translation="MLHAVGSTEFDHAVVMVRDRLDALAPHFERQGFHLSGKAVHNLG
                     SCNRLIVLDDTYIELLGWPPGAPPARKEIADSPLGLDALVFRTNDARATHGRLQAAGF
                     AVNPMQVLTRPAEFDGGAVEACFHTVRFAEQPVAGFRLYFCRHLTPEYVWTPALMRHP
                     NGARSLRRIEVRAADAQAVAQRLALVADVRAVAAEGGWDVPLANLRIHVASDARAGVP
                     RIAALGLENRDGARYALDTGLE"
     gene            complement(4157..4696)
                     /locus_tag="VGC69_16320"
     CDS             complement(4157..4696)
                     /locus_tag="VGC69_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY0296895.1"
                     /translation="MLTAAIPAIAPNASLRALSAAADRTEQEAASALLRRMAAHLRQA
                     DKLLDALPRALRDGSPDRYCRHVAYADPSGRFTIVYLVWRPGQYSPVHGHKTWCAYRV
                     LQGEMSETLYDWDPATQDLSVNSRITRKPGDIFTAMPGLQQTHCLGNGGSGVAISLHV
                     YGVAEAHIATGVNLLPARQ"
     gene            4770..5288
                     /locus_tag="VGC69_16325"
     CDS             4770..5288
                     /locus_tag="VGC69_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0296896.1"
                     /translation="MPAAFGPEGNFFPPAPRRPPVDQTDIKILSIMQKNADLSVAEIA
                     EQVNLSVTPCWRRIQKLKEDGVITRNAVVLNPKALGLNLTVFVSIRTSQHNAKWTQNL
                     MGAVMALPNVVEFHRMAGDIDYLLKVVVEDMAAYDRFYRRLIDAVDLLDVSASFSMEI
                     IKSTMELPLNAG"
     gene            complement(5290..5646)
                     /locus_tag="VGC69_16330"
     CDS             complement(5290..5646)
                     /locus_tag="VGC69_16330"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0296897.1"
                     /translation="MPMPADSNIHGDVFGGWIMAQVDIAGAVPASRRAAGRVATISVN
                     AFVFKQPVFVGDLLSFYAKVIKTGTTSITVEVDVYAQRQRLDAEVVKVTEATLTYVAT
                     DEARRSRPLPALPVNP"
     gene            complement(5854..6915)
                     /locus_tag="VGC69_16335"
     CDS             complement(5854..6915)
                     /locus_tag="VGC69_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEY0296898.1"
                     /translation="MTTMSATSLPLSATPSRNRLNGILFVALMAVAVVQLSDLPLIHR
                     LGFSPLVVGIVCGMLYGNFLRGTMPVEWNAGITFTAKRLLRLAVAFYGLNISVQQIVA
                     VGLPGLAVSVAIVLGTLLIGAFVGQRLLGLDRDTALLTASGSAICGAAAVLAFESALR
                     SEPHKSAVAVATVVLFGTLSMFLYPVFYHAGWLHFADSHALGIYIGGTIHEVAQVVGA
                     ASNIDPATTEVATIVKMTRVVLMVPVLLVLGLWLASRTRAAGQDGKQGAAKLPIPWFA
                     VGFLVLGLVNSLGVLPEWLVTAVRRLDVFALAMAMTAMGIETRFAQIRKAGPRVLVLG
                     AILYVWLILGGYAIVQLAV"
     gene            7011..7883
                     /locus_tag="VGC69_16340"
     CDS             7011..7883
                     /locus_tag="VGC69_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296899.1"
                     /translation="MTPDQLLTFAAVADAGNISRAAQVLHLSQPAVSGQLRMLQDWFG
                     EPLYHRQGHGIALTSAGERLAEQARQLRQIYLRAQAVRNSWRDLEAGSLRLGASTTPA
                     SYLLPGLVAEFHRRYPTVSLNLSDGNTQQIVERLPGLDMAFIEGDVPAGLPASTAVHA
                     WHEDEVVAVVQAGHPLAGRQAAALQEIAAWPLVMREPGSGVRRLVEQACAEAGLSVAA
                     GLELAGVEGVKQAVRAGLGVGFVSALSMRHEGGALVAVHVAPRPLTRTLNILVPHADT
                     SARATQRFLALCLV"
     gene            complement(7932..9137)
                     /locus_tag="VGC69_16345"
     CDS             complement(7932..9137)
                     /locus_tag="VGC69_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY0296900.1"
                     /translation="MKLIEPIVAWQQEIARVRRDIHAHPELSYEEFRTADIVAAKLQE
                     WDIDIDRGLGGTGVVGIIRGKQPEDRANPRAIGLRADMDALPMQELNTFEHRSQNNGK
                     MHACGHDGHTAMLLGAARYLSQHRDFAGTVYLIFQPAEEGGAGAQRMIQDGLFTKFPM
                     QAVFGMHNWPGFAAGSFGVTAGPIMASANEFSIVVKGKGAHGGMPHLGNDPIMAAAQM
                     ALSMQTIVSRNRHPLEGAVLSITQIHAGSADNVIPTDAELRGTVRTFTTEMTDLIERR
                     MREIAEHTAAAMNCEVDFVFNRNYPPTINDAEEAAFCAEVMRGIVGEANVDDHVTPTM
                     GAEDFSYMLLEKPGCYVWIGNGDGDHREHGHGMGPCTLHNGSYDFNDELLPLGATYWV
                     ELALKRLAK"
     gene            9225..10886
                     /locus_tag="VGC69_16350"
     CDS             9225..10886
                     /locus_tag="VGC69_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0296901.1"
                     /translation="MLRPWLAHYPAEVPAEIPPHDYASLTDLLDRACRRHAGRTVCRF
                     MGSDISYAELDAKAAAFAGWLQARGLRRGERVALMMPNVPAYFVALLGVLRAGLAVVN
                     VNPLYTANELGHQLRDCGAGAIVILESFAHVLQRTENIPDLRHVVVVAPGDMLGAFKA
                     PLVNFIAKRVKKRVPAWRIEGAHRWAGVMRSRKPYAPVPVQADDLAVLQYTGGTTGLP
                     KGAMLTHGNLVANVLQVAAVARPALGDLGGPPQTILGALPLYHVFAMTVCGLFCMHAG
                     MRNLLIINPRDRTALIHAWRKTPVHLFPGVNTLFNGLLSHTQFASLDFSALRMTFAGG
                     MAVQASVAEHWSRVTGGALIEGYGLSETSPVATVNPTNSTSYSGTAGLPLPSTDVAIL
                     DEAGAALPIGEVGEVGIRGPQVMKGYWRQPEETWAVMTPDGFFRTGDIGVLDERGCLS
                     LVDRKKDMITVAGFKVYPNEVEALVATHPGVLECAVVGVPSRHSGESVRLYVVRRDPA
                     LTQGQLMAWCRQRLVGYKRPRRVIFRDELPKTNVGKILRRALREA"
     gene            complement(10907..11776)
                     /locus_tag="VGC69_16355"
     CDS             complement(10907..11776)
                     /locus_tag="VGC69_16355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0296902.1"
                     /translation="MQNTTTSKHSSIDLGAAIDAAVCAAHAGAAILQSYSHNRADLVI
                     DHKARNDLVSQADREAEAAVIEVLKSRMPGLGIVAEETGGVPQGPATWYIDPLDGTTN
                     YLHGIPQYAVSIALIAHAGTPLSVGEPIAVDTPVVGVIYDPCREELFTGVYGIGSWLN
                     GRRISCSRTAGLSEAVVATGFPFRDFTFADQYMPMLHAAINTSRGVRRLGAAALDLAW
                     TACGRFDGYWEMGLAPWDVAAGTLIVREAGGRCTDMHLADPWPIGGRVVAGNPAVYKD
                     LAAMIAPHLEPLA"
     gene            11903..13762
                     /gene="mnmC"
                     /locus_tag="VGC69_16360"
     CDS             11903..13762
                     /gene="mnmC"
                     /locus_tag="VGC69_16360"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEY0296903.1"
                     /translation="MSSSYIPLQPADLSLDEAGRPYSVSYGDVYHSRAGALGQAEQVF
                     LRGNGLPQRWRGRSRFTVCETGFGLGLNFLALWRAWRADPQRPARLHMVSIEGHPFEQ
                     AALREAWSRHMPQELQAYADQLAAQWPPLLPGLHRLEFEDGAVTLTLAFGEAAIVAPR
                     LSARVDAYFFDGFAPSRNPAMWSPELLGTLSRLAAPDATLATWASAGHVRRALAQAGF
                     AVTRQPGYGKWHMTVGARAPQTGPGPDLPDDDAASVLVVGAGLAGAGVARALALRGRR
                     VTVLAPEANAGAHRGHLAAALTPVIARDDNPRARLSRAGSLRALRRWAGLDDAVRRCG
                     TLQLERDAGRAAALAQALADLAFPASWARAVDADEAGRLAGLAVSRGGVYFHDGLLVR
                     PDRLVSALLSGPGLVRIAGTAAGIRPAGGLWQACDAQGRVLAEASQVVLACAAQVPAL
                     LQASGLLEPLPRLAQMHALAGEVTQVPAAVLQGGPRCIVGGEGYLLPEVAGWCVAGST
                     YVHGASQAQVEPQGQRVNLDKAAGLLGVEHGRALRTLPVGGLPGWAGWRAVLPGRLPA
                     VGPLAHARGVWLATGYASRGLSWSALAGDLIAAWLCGEPLPLETDLIAQILPR"
     gene            13928..16141
                     /locus_tag="VGC69_16365"
     CDS             13928..16141
                     /locus_tag="VGC69_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEY0296904.1"
                     /translation="MPPKSDLAQFVPLETAELLTSRPSRFDFDAAGGLHLVIEAHAPG
                     VFRLRCGDPALLSDEKASARTRALAEMLLARQEAVGEATAAPYSGGWRIEQGDVTLDV
                     LLEPLRIVLSRGGHVVLASDASETAFAHYALDDADAATWTAAFALKSGERIYGLGETP
                     GDLNRRGEAVVSDDPEHRALPLAWSPRGWGVYANTLRRVEHAPGIAPAGSAYVLTLDD
                     PVLDLFLFAGEPGEILNQYTALTGRAGQPGLWAMGAWLQQAPGEMPTQTAGLIERLRT
                     HQIPLDVVSLALPAAWGFQADKPVLEWDSQRFPDAKQMLALFHKHHVHVSAPGFPGVS
                     QGSPLFEELEDRGWLLANDAGGAQVFEGHPATGGQPYGLLDLTHRDAYVLWVERHRQQ
                     VEDGLDAPACSAQIDIPDGVSARSGESGASLRTQYPLLVRRALFDAVAGHKVPPEGVI
                     PSTDLFPAAQRLPWQAGPQVPNSWAGLEHSLRTALSVGASGLPVQTHNIGSVSAPLDG
                     MDAELYLRWLAMGVFSANFSFQGIDGLMPWAFGDAVLGQVRTWMNWRYRLIPYVLGAI
                     EDAARTGLPVQRSMAMSFPNDPDAHAWDLQYLLGPALLVAPITQPGSEVKVYLPKGEA
                     WWDLNTGHRYEGGTVWTLACGPEQYPVFGREGHMLCLGPLAQHAGEFNSARILDEVWM
                     FGMPLHNPVVMRNKIRVMQMQGSSYIKGLEGLKILPSEGLEVKRRGAEVRISRAR"
     gene            16399..>17375
                     /locus_tag="VGC69_16370"
     CDS             16399..>17375
                     /locus_tag="VGC69_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0296905.1"
                     /translation="MIHIQNLSKTYATPHGRFEALKGIDLRIEQGEVFGIIGPSGAGK
                     STLVQCINLLERPDQGSITLGGQSLTGLSESQLRAQRRRIGMVFQGFNLLARRTVYAN
                     VALPLEIAGVPAAGIPARVERLLELVGLSHLRDHYPSQISGGQKQRVGIARALANKPD
                     VLLSDEATSALDPETTHNILALLRDINRKTGVTVVMITHQMEVVREICDRVAVLSQGR
                     VVEVGPTHEIFATPSHDVTRAMVSAATASDLTGATLDVVRERIAALPPGRAARLLKLS
                     LTGSSAGSLLSDLARDYGLDLNLVQARVEDIQGVAVGTVFVLAQGAQQT"
BASE COUNT         2695 a         5750 c         6188 g         2742 t
ORIGIN      
        1 aggggccgag ggcgcctgat ggcgcgcccg agcccctccg atcaggccgg cccctcaggc
       61 cccaacgccg ctgcgtctca agaacccttc ttatgatgcg ccagcaaacg cgcaatcgca
      121 tccgccaagg gcccgggccg cacgcttttc ccaaggtcat cgaaggcttg cagggcatcc
      181 gcccccagcg gctggaccgt cttgggcagg cgggcggcac ggctgccggg gcgcggcatg
      241 ccggcctgga tgcgcacggc gatctcgttg agcagccagc cgcgcgtggc cagcgcctgg
      301 gccaggctgg gcgccatctg gcgcagcttg gcggcgtagg cggagcccgg aaccgccagt
      361 tccaggcgct ggttgtcgag cttgatgacc gcgcagacct cgcccaggcc cggcggcagc
      421 aaggcggcca gggcctgctg tatctgcagg tggcgctgcg ccgtggccag caccccggcg
      481 ccacgcgtgt cgtggcccag ccaggacagg gcggtttcct gggggcggcc ggccttggag
      541 cgagggcgtg gggaaaaagg cttgttcatg cgcgggcagg gcttgatggg tgtgattatg
      601 cccttacctg cttgaaggcg tacccaggtg acggtaaccc gggtgcgtta cactcgactg
      661 tttccttcgt gccgtaccag agacgcatga tttccctgct caaaaaactc ataggcagcc
      721 gcaatgaccg gctgctgaag caataccgca agcttgtcgg ccagatcaac gcgctcgaac
      781 cgcaaatggc cgcgctgtcg gacgaggcgc ttgccgccaa gacccaggaa taccgttccc
      841 gcatcgagca gggcgccacg ctcgacgacc tgctgcccga ggctttcgcc acggtgcgcg
      901 aggccggcaa gcgggtgttc ggcatgcggc acttcgacgt gcagctgttg ggcggcatcg
      961 ccttgcacaa cggcaagatc gccgaaatgc gcaccggcga aggcaagacg ctgatggcca
     1021 cgctgccggt ctacctgaac gcgctggccg gcaagggcgt gcacgtggtc acggtcaacg
     1081 actacctggc ccgccgcgac gcggaaacca tgggccagct gtaccgcttc ctgggcctgt
     1141 ccaccggcgt ggtggtgccg cagcaggaaa acgaggaaaa gatcgcgtcc taccgcgccg
     1201 acatcaccta cggcacgaac aacgaattcg gcttcgacta cctgcgcgac aatatggagt
     1261 accgcgtcga ggaccgccgc cagcggccgc tcttctacgc catcgtcgac gaagtggact
     1321 ccatcctgat cgacgaggcg cgcacgccgc tcatcatctc gggccaggcc gaggaccata
     1381 ccgacctgta cgtgcgcatg aactcggtgc cgccgatgct cgcgcgcatg gccgccgagc
     1441 ccaagcccca ggaacccgag cccgagggcg actactgggt cgacgagaag agccagcagg
     1501 tctacctgtc cgaggccggc cacgagcacg ccgaggagct cctctcccag agcggcatcc
     1561 tgccggccgg ggaatcgctc tacgacccgc gccacatcgc gctcatgcac cacctcatgg
     1621 tcgccctgcg cgcccatacg ctgttcttcc gcgaccagca gtacgtggtg caggacggcg
     1681 aggtggtcat cgtcgacgaa ttcaccggcc gcctgatggt cggccgccgc tggtccgacg
     1741 gcctgcacca ggccgtcgag gccaaggaag gcgtgaagat cgagaatgaa aaccagaccc
     1801 tggcctcgat caccttccag aactacttcc gcatgtacga gaagctggcg ggcatgacgg
     1861 gcacggccga caccgaggcc tacgaattcc aggagatcta caccctggaa acggtcatca
     1921 tcccgaccaa caagcccatg gtccgcaagg accagaacga ccaggtcttc aagacccacc
     1981 aggaaaagta caacgccatc ctggaagaca tccgcgactg ccatgcgcgc ggccagccgg
     2041 tgctggtggg caccaccagc atcgagaact ccgaactgct gtcgagcttg ctcaatcagg
     2101 ccaagctgcc gcacgccgtg ctcaacgcca agcagcacgc ccgcgaggcc gagatcgtgg
     2161 ccgaggccgg caagccgggc catatcacca tcgccaccaa tatggcgggc cgcggcaccg
     2221 acatcgtgct gggcggcagc atggacaagc agatcgccct gatccgcgcc gacgagtcgc
     2281 tgtccgaggc cgatcgcgag gcgcgcatcc agcaggtgcg cgccgactgg aaaccggcca
     2341 acgagcaggt caaggccgcc ggcggcctgc gcatcatcgg caccgaacgc cacgagtcgc
     2401 gccgcatcga caaccagctg cgcggccgcg ccggccgcca gggcgatccg ggctcgtcgc
     2461 gcttctacct gtcgctcgaa gatccgctga tgcgcatttt cgcgggcgac cgcgtgcgca
     2521 gcatcatgga ccgcctcaag ctgcccgaag gcgagcccat cgaggccggc atggtgacgc
     2581 gctcgatcga gaccgcgcag cgcaaggtcg aaggccgcaa cttcgacatc cgcaagcagc
     2641 tgctcgaata cgacgacgtg gccaacgacc agcgcaaggt gctctacgcc cagcgcaacg
     2701 aggtgctcga ggccggctcg atcagcgaga ccgtcgccag cctgaccgag tcggtcatca
     2761 ccgacctgtt ccatgcccac gtcccgccgg agtcggtgga agaccagtgg gacgtggccg
     2821 gcctgcagca ggtgctggcc aacgattggc agctgcagct gccgctgacc gacatgctcg
     2881 aggaagagcc cgacctggcc gaggaagacc tgcgcgagcg ggtgctggcg gcggcgcgcg
     2941 aagcctatgc gtccaagatc gccctggtcg gccaggagtc gtggtcgcag ttcgagcgtt
     3001 ccatcatgct gcaggccatc gactcgagct ggcgcgaaca cctgtcggcg ctggattacc
     3061 tgcgccaggg catccacctg cgcggctatg cgcagaagaa tcccaagcag gaatacaagc
     3121 gcgaggcgtt cgaactgttc tccggcatgc tcgaccgcat ccgcaacgat gtggtgcgcg
     3181 tgctgctgac ggtgcgcgtg cagtcggccg aacagctcga agaggccgag ggcgagaccg
     3241 agcagatgca gaacctgcag ttccagcaca gcgatcccga cgccgaactg gccgccgtgg
     3301 cggcgccgca ggcggcggct gtgccggccg gcgccgtccc gcgcgtgggc cgcaacgatc
     3361 cttgcccctg cggcagcggc aagaaataca agcagtgcca cgggaagctc acctgatagg
     3421 gagaccgcca tgttgcatgc cgtcggcagc accgagttcg atcacgccgt ggtcatggtg
     3481 cgcgaccggc tggatgcgct ggccccgcat ttcgagcgcc agggctttca cctgagcggc
     3541 aaggcggtgc acaacctcgg gtcgtgcaac cgcctcatcg tgctcgacga cacctatatc
     3601 gagctgctcg ggtggccgcc cggcgctccg ccggcgcgca aggaaatcgc cgattcgccg
     3661 ctggggctgg acgcgctggt gtttcgcacc aacgatgcgc gggccaccca cggccgcctg
     3721 caggcggccg gcttcgcggt gaaccccatg caggtgctga cccgtccggc cgagttcgac
     3781 ggcggcgcgg tggaggcctg ttttcatacc gtgcgtttcg ccgagcagcc ggtggcgggt
     3841 tttcgcctgt atttctgccg gcacctcacg cccgagtatg tgtggacgcc cgccttgatg
     3901 cggcacccca acggcgcccg cagcctgcgc cgcatcgagg tgcgcgcggc cgatgcccag
     3961 gcggtggccc agcgcctggc gctggtggcc gacgtgcggg ccgtggccgc cgaaggcggc
     4021 tgggacgtgc cgctggccaa tttgcgcatc cacgtggcca gcgatgcgcg ggccggcgtg
     4081 ccgcgcatcg ccgcgctggg cctggaaaac cgcgatggcg cccgctatgc gctggacacc
     4141 gggctggaat agcggattac tgccgcgcgg gcagcaggtt cacgccggtg gcgatgtgcg
     4201 cttcggccac gccgtagaca tgcaatgaga tggccacgcc gctgccgccg ttgcccaggc
     4261 aatgggtctg ctgcaggcca ggcatggccg tgaagatatc gcccggcttg cgggtgatcc
     4321 ggctgttgac cgacaggtcc tgggtggccg ggtcccagtc gtagagggtt tccgacattt
     4381 cgccctgcag cacgcggtag gcgcaccagg tcttgtggcc gtgcaccggg ctgtattggc
     4441 ccgggcgcca caccagatag acgatcgtga agcggccgct cgggtcggcg taggccacgt
     4501 ggcggcagta gcggtcgggc gacccgtcgc gcagcgcccg cggcagggcg tccagcagct
     4561 tgtcggcctg ccgcaggtgc gcggccatcc gccgcagcag ggccgaggcg gcttcctgct
     4621 cggtcctgtc ggcggcggcg gacagggccc gtagcgaggc gttcggggcg atggcgggta
     4681 tggcggcggt tagcatggca atatcgggta aacgcgggat aggagcattt ccatatccta
     4741 aagtgccggg tatggaataa atttgcctaa tgccggcggc tttcgggcca gagggaaatt
     4801 tttttccgcc ggcgccaagg agacctcccg tggaccagac ggacatcaag atcctgagca
     4861 tcatgcagaa aaatgccgac ctgtcggtgg ccgagatcgc cgagcaggtc aacctgtcgg
     4921 tgacgccatg ctggcggcgg attcaaaagc tcaaggaaga cggggtcatc acccgcaacg
     4981 cggtggtgct caaccccaag gccctggggc tcaacctgac ggttttcgtg tccatccgca
     5041 ccagccagca caatgccaag tggacgcaga acctcatggg cgccgtgatg gcgctgccca
     5101 atgtggtgga gttccaccgc atggcgggcg acatcgacta cctgttgaag gtggtggtcg
     5161 aggacatggc cgcctacgac cgcttctacc gccgcctgat cgacgcggtc gacctgctgg
     5221 acgtcagcgc cagcttttcc atggaaatca tcaagagcac catggagctg ccgctgaacg
     5281 ccgggtgact tacgggttga ccgggagcgc cggcaggggg cggctgcggc gggcctcgtc
     5341 ggtggccacg taggtcagcg tcgcctcggt gaccttgacg acctcggcgt ccaggcgctg
     5401 gcgctgggcg tagacgtcca cttcgacggt gatcgaggtg gtgccggtct tgatgacctt
     5461 ggcgtagaag ctgagcaggt cgcccacgaa caccggctgc ttgaacacga aggcgttcac
     5521 ggagatggtg gccacccggc cggccgcgcg gcgcgaggcc gggacggcgc ccgcgatgtc
     5581 gacctgcgcc atgatccagc cgccaaacac gtcgccgtgg atgttcgagt ccgccggcat
     5641 gggcatgacg cgcaggaccg gctgctcgtc gggcagcgtg acgaagggat gggggttggg
     5701 cgcagtggac acggttttgc tccgatggat gggacgaccg ccgcacgggg cgggaaccat
     5761 tgattatgca gcaaaaccct ggcgcccgtg cccgccggaa gggcgccgcg gccgatggcc
     5821 gagccgccgc ggggccggcc gtgcaagcgg ctgtcagacg gccaactgca cgatggcgta
     5881 gccgcccaag atcagccaga catagagaat ggcgcccagc accagcacgc gggggccggc
     5941 cttgcggatc tgggcgaagc gggtctcgat gcccatggcg gtcatggcca tggcgagcgc
     6001 gaagacatcc aggcggcgca cggcggtgac gagccactcg ggcagcacgc ccagcgagtt
     6061 caccaggccc aggaccagaa agcccacggc aaaccagggt atgggcagtt tggcggcgcc
     6121 ctgcttgccg tcctggccgg cggcgcgggt gcggctggcc agccacaggc ccagcaccag
     6181 gagcaccggc accatcagca ccacgcgggt catcttgacg atggtggcga cttcggtggt
     6241 ggccgggtcg atattgctgg ccgcgccgac gacctgggcc acttcgtgga tggtgccgcc
     6301 gatgtagatg cccagcgcgt ggctgtcggc gaagtgcagc cagcctgcgt gatagaacac
     6361 cgggtagagg aacatcgaca gcgtgccgaa cagcaccacg gtggcgacgg ccacggcgct
     6421 tttgtgcggc tcggagcgca gcgcggattc gaaggccagc accgcggcgg cgccgcagat
     6481 ggcgctgccc gaggcggtga gcagtgcggt gtcgcggtcc agtcccagga ggcgctggcc
     6541 cacgaaggcg ccgatgagca gggtgcccag cacgatggcc acggatacgg ccaggcccgg
     6601 caggccgacg gcgacgatct gctgcacgct gatgttcagg ccgtagaacg ccacggccag
     6661 gcgcagcagg cgcttggccg tgaaggtgat gccggcattc cactcgaccg gcatggtgcc
     6721 gcgcaggaag ttgccgtaca gcatgccgca gacgatgccg accaccagcg gcgagaagcc
     6781 caggcggtgg atgaggggca ggtcggacaa ctgcacgacg gccacggcca tcagggcgac
     6841 gaacaggatg ccgttgagcc ggttgcggct gggcgtggcg gacaggggca gggacgtggc
     6901 gctcatggtg gtcaaataag taaataacct ggagaaatat taattcccgg ccaattatct
     6961 gaaaaatctt tattttttat agttatcatc ggattttcag atatctggcc atgactccgg
     7021 atcaactgct caccttcgcc gccgtggcgg atgccggcaa catcagccgg gccgcccagg
     7081 tgctgcacct gtcccagccc gccgtgtcgg gccagttgcg catgctgcag gactggttcg
     7141 gcgagccgct ctatcaccgc caggggcatg gcatcgccct gacgtccgcc ggcgaacgcc
     7201 tggccgagca ggcgcggcag ctgcggcaga tctatctgcg ggcccaggcc gtgcgcaact
     7261 catggcgcga cctcgaggcc ggttcgctgc ggctgggggc cagcacgacg ccggccagct
     7321 atctgctgcc cggactggtg gccgaattcc accgccgcta tcccacggtc agcctgaacc
     7381 tgtccgacgg caacacgcag cagatcgtcg agcgcctgcc cgggctggac atggccttca
     7441 tcgagggcga cgtgcccgcg ggcctgccgg ccagcacggc cgtgcatgcc tggcacgagg
     7501 atgaggtcgt ggccgtggtg caggccgggc atccgctggc cggccggcag gccgccgccc
     7561 tgcaggagat cgccgcctgg cccctggtga tgcgcgagcc gggctcgggc gtgcgcaggc
     7621 tggtcgagca ggcctgcgcc gaggccggcc tgtcggtggc ggccgggctg gaactggcag
     7681 gggtggaagg cgtgaagcag gccgtgcggg ccggcctggg ggtgggtttc gtgtcggcgc
     7741 tgtcgatgcg gcacgagggc ggcgcgctgg tcgcggtgca cgtggcgccc cggccgctga
     7801 cgcggaccct gaacatcctg gtgccgcacg ccgatacgtc ggcccgcgcc acgcagcggt
     7861 tcctggcgct gtgcctggtg tgatgcgggc atggccggct cctggggcgg agccggccat
     7921 gcccgtccgg cttacttggc caggcgcttg agcgccagct cgacccagta ggtcgcgccc
     7981 agcggcagca gctcgtcgtt gaagtcgtag ctgccgttgt gcagggtgca ggggcccatg
     8041 ccgtggccgt gctcgcggtg gtcgccgtcg ccgttgccga tccagacgta gcaaccgggt
     8101 ttttccagca gcatgtagga gaagtcctcg gcgcccatgg tcggcgtgac gtggtcgtcc
     8161 acgttggcct cgcccacgat gccgcgcatc acttccgcgc agaaggcggc ttcttccgcg
     8221 tcgttgatgg tgggcggata gttgcggttg aagacgaagt cgacttcgca gttcatcgcg
     8281 gcggccgtgt gttcggcgat ctcgcgcatg cggcgctcga tgaggtcggt catctcggtg
     8341 gtgaaggtgc gcacggtgcc gcgcaattcg gcgtcggtgg ggatgacgtt gtcggcgctg
     8401 ccggcgtgga tctgcgtgat gctgaggacc gcgccttcga gcgggtggcg gttgcgcgag
     8461 acgatggtct gcatggacag cgccatctgc gcggccgcca tgatggggtc gttgcccagg
     8521 tgcggcatgc cgccgtgggc gcccttgccc ttgacgacga tggagaactc gttggccgag
     8581 gccatgatgg ggccggccgt cacgccgaag ctgccggccg caaagccggg ccagttgtgc
     8641 atgccgaaca cggcttgcat ggggaacttg gtgaacaggc cgtcctggat catgcgctgg
     8701 gcgccggcgc cgccttcctc ggcgggctgg aagatcaggt agaccgtgcc ggcgaagtcg
     8761 cggtgctgcg acaggtagcg cgccgcgccc agcagcatgg cggtgtggcc gtcgtggccg
     8821 caggcgtgca tcttgccgtt gttctggctg cggtgttcga aggtgttgag ctcctgcatg
     8881 ggcagggcgt ccatgtcggc gcgcaggccg atggcgcgcg ggttggcccg gtcctcgggc
     8941 tgcttgccgc ggatgatgcc gaccacgccg gtgccgccca ggccgcggtc gatgtcgatg
     9001 tcccattcct gcagcttggc ggcgacgatg tcggccgtgc ggaattcttc gtaggagagc
     9061 tcggggtgcg cgtggatatc tcgccggacg cgggcgatct cttgctgcca ggcgacgatg
     9121 ggttcgatga gtttcatggg ggatgaagcg cgtgccgcgc tgtgagaaaa gtacttccca
     9181 agggtatcat caacgcccaa aattcataat ccggagactt ccttatgctg cgtccctggc
     9241 tggcgcatta tccggcggag gtgccggccg agatcccgcc gcatgactac gcctcgctga
     9301 ccgacctgct cgaccgcgcc tgccggcggc atgcggggcg caccgtctgc cgcttcatgg
     9361 gcagcgacat cagctatgcc gaactcgatg ccaaggccgc ggccttcgcg ggctggctgc
     9421 aggcgcgcgg cctgcggcgc ggcgagcggg tggcgctgat gatgcccaat gtgccggcgt
     9481 atttcgtcgc cctgctgggc gtgctgcgcg cgggcctggc cgtggtcaac gtcaacccgc
     9541 tctataccgc caacgagctg gggcaccagc tgcgcgactg cggcgccggc gccatcgtga
     9601 tcctcgagag cttcgcgcac gtgctgcagc gcacggaaaa catccccgac ctgcgccatg
     9661 tggtggtggt cgcgccgggc gacatgctgg gcgcgttcaa ggcgccgctg gtcaatttca
     9721 tcgccaagcg cgtcaagaag cgcgtgccgg cctggcgcat cgagggcgcg caccgctggg
     9781 ccggcgtgat gcgctcgcgc aagccttatg cgccggtgcc cgtgcaggcg gacgacctgg
     9841 ccgtgctgca gtacacgggc ggcacgaccg gcctgcccaa gggggcgatg ctcacgcacg
     9901 gcaacctggt ggccaacgtg ctgcaggtgg cggccgtggc ccgcccggcc ctgggcgatc
     9961 tgggcggccc gccgcagacg atcctggggg cgctgccgct ctaccacgtg ttcgccatga
    10021 cggtatgcgg cctgttctgc atgcacgcgg gcatgcgcaa tctgctcatc atcaatccgc
    10081 gcgaccgcac cgccctgatc catgcctggc gcaagacgcc cgtgcacctt ttcccgggcg
    10141 tcaacaccct gttcaacggg ctgctgagcc acacgcagtt cgccagcctg gatttctccg
    10201 cgctgcgcat gacgttcgcg ggcggcatgg cggtgcaggc ctcggtggcc gagcactggt
    10261 cccgggtgac gggcggggcc ctgatcgaag gctacggcct gtcggaaacc tcgccggtgg
    10321 ccacggtcaa tcccaccaac agcacgtcct acagcggcac cgccggcctg cctttgccct
    10381 cgacggacgt ggccatcctg gacgaggccg gcgcggcctt gccgatcggc gaagtgggcg
    10441 aggtgggcat acgcggcccg caggtcatga agggctattg gcggcagccc gaggaaacct
    10501 gggccgtcat gacgcccgac ggctttttcc gcacgggcga tatcggcgtg ctggacgagc
    10561 gcggctgcct gagcctggtc gaccgcaaga aggacatgat caccgtggcc gggttcaagg
    10621 tgtaccccaa cgaagtcgag gcgctggtcg ccacgcaccc cggcgtgctg gagtgcgccg
    10681 tggtgggcgt gcccagccgg cattcggggg agtcggtgcg cctgtatgtc gtgcggcgcg
    10741 atccagccct gacccagggg cagctgatgg cctggtgccg ccagcggctc gtgggctaca
    10801 agcggccgcg ccgggtgatt tttcgcgacg aattgcccaa gaccaatgtg ggcaagatac
    10861 tgcgccgggc cttgcgcgag gcctagctgg ccccgcctgg cctgcctcag gccaggggct
    10921 cgaggtgcgg cgcgatcatg gcggcgaggt ctttatagac ggcggggttg cccgccacca
    10981 cgcgcccgcc gatgggccag gggtcggcca ggtgcatgtc agtgcagcgg ccgcccgctt
    11041 cgcgcacgat cagcgtgccg gccgccacgt cccagggggc caggcccatt tcccagtagc
    11101 cgtcgaagcg gccgcaggcc gtccaggcca ggtccagcgc ggccgcgccc aggcggcgca
    11161 cgccgcgcga ggtgttgatg gcggcatgca gcatgggcat gtactggtcg gcgaaggtga
    11221 agtcgcggaa cggaaagccc gtggccacga cggcttcgct caggcccgcc gtgcgcgagc
    11281 agctgatgcg ccggccgttg agccaggagc cgatgccgta gacgccggtg aacagctctt
    11341 cgcggcaggg gtcgtagatc acgccgacca cgggggtgtc gaccgcgatg ggctcgccca
    11401 ccgacagcgg cgtgccggcg tgcgcgatca gcgcgatgga cacggcgtat tgcggaatgc
    11461 catgcagata attggtggtg ccgtcgagcg ggtcgatgta ccaggtggcc gggccttgcg
    11521 gcacgccgcc ggtttcttcc gccacgatgc ccaggcccgg catgcgggac ttgagcacct
    11581 cgatgacggc cgcctcggct tcgcggtcgg cctgggagac caggtcgttg cgcgccttgt
    11641 ggtcgatgac gaggtcggcg cgattgtgcg agtacgactg caggatggcc gcgccggcat
    11701 gcgccgcgca gaccgccgca tcgatggccg cgcccaggtc gatcgatgaa tgcttggaag
    11761 tcgtcgtgtt ctgcatgcct gcagtgtaag cccgccgatg ttgccgggtc gcgtagtcgg
    11821 gcgtgattgc gcgcttgcgc gtggcgcaca gccaacgttg ttgcaggctt ctggtatgtt
    11881 ggcggcaact ttgcgatttt ctatgtcttc ttcctacata cctttgcagc cggccgatct
    11941 gtcgctggat gaagcggggc gtccctacag cgtgtcctac ggcgatgtct atcattcgcg
    12001 cgccggcgcg ctgggccagg ccgagcaggt gttcctgcgc ggcaacgggc tgccgcagcg
    12061 ctggcgcggc cgcagccggt tcacggtgtg cgagaccggg ttcgggctgg gcctgaactt
    12121 cctggcgctg tggcgcgcct ggcgggccga tccgcagcgt ccggcgcggt tgcacatggt
    12181 ctcgatcgaa gggcatcctt tcgagcaggc tgccttgcgc gaggcatgga gccggcatat
    12241 gccgcaggaa ctgcaggcct acgccgacca gttggccgcc cagtggccgc cgcttctgcc
    12301 tggcctgcac aggctggaat tcgaggatgg cgccgtcacg ctgacgctgg ctttcggcga
    12361 ggcggccatc gtcgcgccgc gcctgtcggc gcgcgtcgac gcctattttt tcgatgggtt
    12421 cgcgcccagc cgcaacccgg ccatgtggtc gcccgagttg ctgggcacgc tgtcccggct
    12481 ggctgcgccc gacgccacgc tggccacctg ggccagcgcg ggccatgtgc ggcgggcgct
    12541 ggcccaggcc ggttttgccg tgacgcgcca accaggctat ggcaagtggc atatgacagt
    12601 gggcgcgcgc gcaccgcaaa ccgggcccgg cccggacctg cccgacgatg acgcggcgtc
    12661 ggtgctggtg gtcggcgcgg gcctggccgg cgccggcgtg gcgcgggccc tggcgctgcg
    12721 cggccggcgc gtgacggtgc tggcccccga agcgaacgcc ggcgcgcatc gcggccacct
    12781 ggcggccgcg ctgacgccgg tgatcgcgcg cgacgacaat ccgcgagccc ggctgtcgcg
    12841 ggccggcagc ctgcgcgcct tgcggcgctg ggccggcctg gacgacgccg tgcgtcgctg
    12901 cggcaccctg cagctcgagc gcgatgccgg acgcgccgcg gccctggcgc aagccttggc
    12961 cgacctggct ttcccggcat cgtgggcgcg ggccgtcgat gcggacgagg cagggcgcct
    13021 ggcgggcctg gcggtgtcgc gcggcggcgt ctactttcac gatggcctgc tggtgcggcc
    13081 cgaccggctg gtgtcggcct tgctgtccgg gccgggcctg gttcgcatcg ccgggacggc
    13141 ggcgggcatc aggccggccg ggggcctgtg gcaagcctgc gatgcgcaag gccgggtgct
    13201 ggccgaagca agccaggtcg tgctggcctg cgccgcgcaa gtgccggcgc tgctgcaggc
    13261 cagcggcttg ctcgaacccc tgcccaggct ggcgcagatg catgccctgg ccggcgaggt
    13321 gacgcaggtg cccgcggccg tgttgcaggg cgggccgcgc tgcatcgtgg gtggcgaggg
    13381 gtatttgctg cccgaggtgg cgggctggtg cgtggcgggc agcacctatg tgcacggcgc
    13441 cagccaggcg caagtcgagc cgcaggggca gcgcgtcaac ctggacaagg cggcaggttt
    13501 attgggggtg gagcatgggc gggccttgcg cacgctgccg gttggcggcc tgcctggctg
    13561 ggctggctgg cgggccgtgc tgcccggccg gctgccggcc gtaggacccc tggctcacgc
    13621 ccgcggcgtc tggctggcga ccggctatgc ctcgcgcggt ttgagctggt cggccctggc
    13681 gggagacctg attgccgcct ggctgtgcgg cgaaccgctg cccctggaaa ccgacctgat
    13741 cgcgcagatt ctgccgcgtt aatgacgcca taaagcccgc atcgcagccg gtccgggcac
    13801 cgcggatttg tggcgcgatt cggacccccg cgcagggctc gaggggcgcc gagtttattt
    13861 caaaagacaa atccgtcaaa atagaggctt ttgtagcttt ttggcccact gctgggggat
    13921 gccttccgtg ccgcctaagt ctgacttagc ccaatttgtc ccgcttgaaa ccgctgagct
    13981 gttgacgagc cgtcccagcc gcttcgattt cgatgcggct ggcggcttgc acctggtgat
    14041 cgaggcccat gcgcccggcg tgttccgcct gcgctgcggc gacccggcct tgctgtccga
    14101 cgaaaaagcc agcgcccgca cgcgcgccct ggccgaaatg ctgctggccc ggcaggaggc
    14161 cgtgggcgaa gccaccgccg ccccctactc gggcggctgg cgcatcgagc agggcgacgt
    14221 cacgctcgac gtgctgctcg agcccttgcg catcgtgctg tcgcgcggcg gccatgtcgt
    14281 gctggcttcc gatgcctcgg aaaccgcgtt cgcccactat gcgctggacg atgccgacgc
    14341 cgcgacctgg acggcggcct tcgcgctcaa gtccggcgag cgcatctacg gcttgggcga
    14401 aaccccgggc gacctcaacc gcagaggcga ggccgtggtg tccgacgacc ccgagcaccg
    14461 cgccctgccg ctggcctgga gcccgcgcgg ctggggggtc tatgccaata ccctgcgccg
    14521 cgtcgagcac gcgccgggca tcgcgcccgc ggggtcggcc tatgtgctga ccctcgacga
    14581 tccggtgctg gacctctttc tcttcgccgg cgagcccggc gaaatcctca accaatacac
    14641 ggcgttgacg ggccgcgccg gccagcccgg cctgtgggcc atgggcgcct ggctgcagca
    14701 ggcgccgggc gagatgccca cgcagacggc cgggctgatc gaacgcctgc gcacgcacca
    14761 gatcccgttg gacgtagtgt cgctggcgct gccggcggcc tggggtttcc aggccgacaa
    14821 gcccgtgctc gaatgggaca gccagcgctt tccggatgcc aagcagatgc tggcgctgtt
    14881 ccacaagcac catgtgcatg tgtcggcgcc cggcttcccg ggcgtgtcgc agggcagccc
    14941 gctgttcgaa gagctggaag accgcggctg gctgctggcc aacgacgcgg gcggcgcgca
    15001 ggtgttcgag ggccatccgg ccacgggcgg gcagccctac ggcctgctcg atctcacgca
    15061 ccgcgatgcc tacgtcctct gggtggagcg gcatcgccag caggtcgagg acggcctgga
    15121 tgcgccggcc tgcagcgcgc agatcgacat tcccgacggc gtgagcgccc gcagcggcga
    15181 aagcggcgct tccttgcgca cgcaataccc gctcctggtg cgccgcgcgc tgttcgacgc
    15241 cgtagccggc cacaaggtgc cgccggaagg cgtgattccc agcaccgatc tctttcccgc
    15301 cgcccagcgc ctgccctggc aggccgggcc gcaggtgccc aacagctggg ccggcctgga
    15361 gcacagcctg cgcacggcgc tgtcggtcgg cgccagcggc ctgccggtgc agacgcacaa
    15421 tatcggttcg gtgtcggcgc cgctcgacgg catggacgcc gagctgtacc tgcgctggct
    15481 cgccatgggc gtgttctcgg ccaacttcag cttccagggc atcgacgggc tgatgccctg
    15541 ggctttcggc gacgcggtgc tgggccaggt gcgcacctgg atgaactggc gctaccgcct
    15601 gataccctat gtgctgggcg cgatcgagga cgccgcgcgc acgggcctgc cggtgcagcg
    15661 ttcgatggcc atgtcgttcc cgaacgatcc ggacgcccac gcctgggact tgcaatacct
    15721 gctgggcccg gccctgctgg tggcgccgat cacccagccc ggcagcgagg tcaaggtcta
    15781 cctgcccaag ggcgaggcct ggtgggacct gaacacgggg caccgctacg agggcggcac
    15841 ggtctggacg ctggcctgcg ggccggagca gtacccggtg ttcggccgcg aaggccatat
    15901 gctctgcctg gggccattgg cccagcacgc gggcgagttc aactcggccc gcatcctcga
    15961 cgaggtctgg atgttcggca tgccgctgca caacccggtg gtgatgcgca acaagatccg
    16021 ggtgatgcag atgcaggggt ccagctatat caaggggctg gaagggttga agatcctgcc
    16081 ttccgaaggg ttggaggtga agcggcgcgg ggctgaggtc aggatttccc gggcgcgttg
    16141 atttatttga gttcttgagt cgtttttcgg cctcgggcca agtctgcggc ccgatcggcg
    16201 gggctcgggc gcgccatcag gcgccctcgg cccccttcgg gggcttcccc cgcctcaaag
    16261 ggccggagac ttggcccgag gccgaaaaac tcagatcgga tgataaattc cgcagccttc
    16321 taaataacca aaaataataa aaacagtcca attctgttgt tcttaagtaa atgagggcga
    16381 gtcataatcg cctgtctcat gattcatatc caaaatttat ccaagaccta cgccacgccg
    16441 catggccggt tcgaggccct caagggtatc gacctgcgca tcgagcaagg cgaggttttc
    16501 ggcatcatcg ggccgagcgg cgcaggcaag agcacgctgg tccagtgcat caacctgctc
    16561 gagcggcccg accagggcag catcacgctg ggcggtcaat cgctgacggg actttccgag
    16621 tcgcagctgc gcgcgcagcg ccgccgcatc ggcatggtgt tccagggctt caacctgctg
    16681 gcccgccgca cggtgtacgc caatgtggcg ctgccgctgg aaatcgccgg cgtgccggct
    16741 gccgggatcc ccgcgcgcgt ggagcgcctg ctggagctgg tggggctgtc gcacctgcgc
    16801 gaccactatc ccagccagat cagcggcggg cagaagcagc gcgtgggcat tgcacgcgcg
    16861 ctggccaaca agccggacgt gctgttgagc gacgaggcga cgtcggcgct ggacccggag
    16921 accacgcaca atatcctggc cctgctgcgc gacatcaacc gcaagacggg cgtcacggtg
    16981 gtgatgatca cgcaccagat ggaagtggtg cgcgagatct gcgaccgggt ggccgtgctg
    17041 tcgcagggcc gggtggtcga ggtgggcccc acgcacgaga tattcgccac gccgagccac
    17101 gacgtgacgc gcgccatggt gtcggccgcc acggcttccg acctgaccgg cgccacgctg
    17161 gacgtagtgc gcgagcgcat cgcggccttg ccgccggggc gcgcggcgcg gctgctcaag
    17221 ctgtcgctca cgggcagctc ggccggttcg ctgctgtcgg acctggcgcg cgactatggg
    17281 ctggacctga acctggtgca ggcccgcgtc gaagacatcc agggcgtggc ggtgggcacg
    17341 gtgttcgtgc tggcccaggg cgcccaacag acctt
//
LOCUS       DASWPO010000314        20631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_788042, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000314 DASWPO010000000
VERSION     DASWPO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20631
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788042"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1399)
                     /locus_tag="VGC69_16375"
     CDS             complement(179..1399)
                     /locus_tag="VGC69_16375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296906.1"
                     /translation="MSSRYQLLAVIWITQLVNYMDRVNIAIAGPTIMHSLGISPTSFG
                     FILSAFTLGYLLMQLPGGYLADRFGAKALMIVAPLVWSFFTAMTGMVSSVAGLILVRI
                     CFGAAEGSSNSAYYKIVGDNFSFRERAGANAVWFTALALGPALAAPVGTWLLGLVGWR
                     QLFWWFAAAGLVVALIVLVAVPDQRRLTVLQDTETPAAGLGWRALLALIASRPTSWLL
                     FFGFMGFNMGFWAFLGWLPSYLSIERHIDLKGLGVAASIPYFCGFLGLVLFGWLSNRR
                     LYRHRAALLGSAYVVAACALYLASIAGSAAACVAGLSAVALCLYGSFAPWSSLMLDLA
                     PVAGRATMAGFVNVGGQIGGLLAPSIVGYLVQSTHSFASGFACMMACLVIGGLCFYAL
                     GWAMIRRPAAGLST"
     gene            1775..2926
                     /locus_tag="VGC69_16380"
     CDS             1775..2926
                     /locus_tag="VGC69_16380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0296907.1"
                     /translation="MFVDQTSLLLALGIASFALSCTLLVTWLFTRTDPFILTWALGTF
                     VLVFAFACFSIYAVNFNALLGLVSNFLLTSSFVILYGASCLFTEQRLPIRRVAIIETS
                     CLLLLGLPVAFGFTALGALAGNLLNAGLLLLTARKFWVGRHETPVWIVGIVIFYTLAA
                     LSFLPCAAMIYLKGPLVLRAPPSGWAEDLNSIVGLIGITGIGALSLALNQARSASRHR
                     EEARTDPLTGLLNRRALFDLYGVGTLAKNTAVLVFDLDHFKSINDVHGHGMGDEVLRR
                     FARVMRASLRARDTAARLGGEEFALILPASTPEIIMRLAERIRIGFAAETFQTPSGPL
                     RCTVSAGVAFAVEESEHFDAVLSRADKALYLAKDSGRNHVMAPDLKRVA"
     gene            complement(3042..3368)
                     /locus_tag="VGC69_16385"
     CDS             complement(3042..3368)
                     /locus_tag="VGC69_16385"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEY0296908.1"
                     /translation="MGQLTSIMEANLHPHRLCLRTHVIVECRGKKEDAELELEFRRIC
                     DGANPGNRILPFDIIFADKKTNLAGLQLADLVARPVGRHHIRPQQLNQAGDGVAEGQL
                     EQETLG"
     gene            complement(3784..6234)
                     /gene="gyrB"
                     /locus_tag="VGC69_16390"
     CDS             complement(3784..6234)
                     /gene="gyrB"
                     /locus_tag="VGC69_16390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY0296909.1"
                     /translation="MSDQQNTANENSYGADSIKMLKGLEAVRKRPGMYIGDTSDGTGL
                     HHMVFEVVDNAIDEALAGYCDDIVVTIHTDNSISVTDNGRGIPTAIHKDDEFHRSAAE
                     IVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNALSEWLKLTIHRDGQTHQMEFHRGAR
                     VAPLTVTGSTAKRGTEVRYLADSEIFNNIEYHYEILAKRLRELSFLNNGVKIRLIDQR
                     QGKEENFAFSGGVKGFVEYINRSKTVLHPNVFAVASESNAGGVNVGVEIAMQWNDSYT
                     ETVLCFTNNIPQRDGGTHLTGLRAAMTRVLNKYIADNELAKKAKVETTGDDMREGLAC
                     VLSVKVPEPKFSSQTKDKLVSSEVRPAVEDAVARTLETWLLEHPADAKALCGKIVEAA
                     RAREAARKAREMTRRKSVLEGAGLPGKLADCQEKDPALCELYIVEGDSAGGSAKQGRD
                     RKFQAILPLRGKVLNVEKARFDRLVSSEQIATLITALGTSIGPDFNIDKLRYHRLIIM
                     TDADVDGAHIRTLLLTLLYRQMPELVARGHIYIAQPPLFKVKVGRDERYLKDEVEEAQ
                     YMMQLALKDAKLVPSADAAPITGDALTELAKKYVMADTVISRLARVFDVAALSALAEG
                     VEINLDDADAAAASAQRLARALAGSDVGSGVDVVAEFDEVAEKHRLLVKRMHHGNVRT
                     SVIDADFVRGADYTVLAESARTFLGLIGAGAVVARGEGDKRKEQPVSDFREAMQWLRA
                     EAERGISKQRYKGLGEMNPEQLWETTMDPKVRRLLRVQIEDAIAADEVFTTLMGDDVE
                     PRRAFIESHALLASNIDA"
     gene            complement(6243..7352)
                     /gene="dnaN"
                     /locus_tag="VGC69_16395"
     CDS             complement(6243..7352)
                     /gene="dnaN"
                     /locus_tag="VGC69_16395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY0296910.1"
                     /translation="MQLVQTTRDALLKPLSTVAGIVERRHTLPILANILMRKEGSKVS
                     FIATDLEVQITTHADFGVGPENEATTVAARKLLDILKALPDTGEVKLGLASNKLSVQS
                     AKSRFALQTLAATEFPTVAQPEQWNVSLTLPQRTLRHLFNMVHFAMAQQDIRYYLNGM
                     LLVFEPGFVRSVATDGHRLAHCATAADGIAEKHEVIVPRKTVLEMQRLLEDSDEPVAV
                     DVAPGQIRFRFGDVEFVSKLVEGKFPDFTRVIPTTYTRHFDVSREALQGSLQRAAILT
                     TDKFKGVRLQLSQNVMKISSTNAEQEEAQEELDIDYAHEPLDVGFNVSYLLDVLGNLK
                     AETVRWSVMPDANASALITLPDDEQFKYVVMPMRI"
     gene            complement(7355..8806)
                     /gene="dnaA"
                     /locus_tag="VGC69_16400"
     CDS             complement(7355..8806)
                     /gene="dnaA"
                     /locus_tag="VGC69_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY0296911.1"
                     /translation="MNEFWQTCISRLEQELPPQQISAWIRPLVPLAFDEEQGLLRVAA
                     PNRFKLDWVRKNFSHQIEALASEWYARPVQVSFELPAAAVSRAVPQRAVAGVGAGVAA
                     TVGAGAAMPRPAGMAAPAAAVAPSPVPAPSAASDAAGVVYERSRLNTDLTFENFVTGK
                     ANQLARAAALQVAENPGTSYNPLFLYGGVGLGKTHLIHAIGNAMVAAGTGTHVRYVHA
                     DQYVADVVKAYQRKTFDDFKRYYHSLDLLLIDDIQFFSGKQRTQEEFFYAFEAMVAQR
                     KQIIITSDTYPKEMAGIDSRLTSRFDQGLTVAIEPPELEMRVAILLRKAESEGATMPE
                     EVAFFIAKHLRSNVRELEGALRKVLAYARFHGRDELTVDLSKDALKDLLSVSSGQVTV
                     ENIQKTVADFYKIKVADMYSKRRPANIALPRQVAMYLAKELTQKSLPEIGDLFGGRDH
                     TTVLHAVRKISDARAKQAELNHTLHVLEQTLKG"
     gene            9095..9229
                     /gene="rpmH"
                     /locus_tag="VGC69_16405"
     CDS             9095..9229
                     /gene="rpmH"
                     /locus_tag="VGC69_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY0296912.1"
                     /translation="MKRTYQPSVTRRKRTHGFRVRMKTRAGRAVLNARRAKGRKRLAV
                     "
     gene            9281..9703
                     /locus_tag="VGC69_16410"
     CDS             9281..9703
                     /locus_tag="VGC69_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY0296913.1"
                     /translation="MTRATLPAEARLHRPSEYAAALKGRRLARGAFFIVSAAPLVPAA
                     APVDISAAPLASPPPEPAPPACARLGLVIAKRYAPRAVTRNALKRVVREAFRAQRLAL
                     PARDYVVRLQGKVAPVSLTELKRAARAEVDAHFGRIAR"
     gene            9733..9996
                     /gene="yidD"
                     /locus_tag="VGC69_16415"
     CDS             9733..9996
                     /gene="yidD"
                     /locus_tag="VGC69_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY0296914.1"
                     /translation="MTRLLLIAPIRFYRFFLSPWIGRQCRYTPTCSAYAIEAIERHGA
                     WTGLWLAVRRIGRCHPWASGGHDPVPDHPASHKGCRRHHPKPD"
     gene            10060..11736
                     /gene="yidC"
                     /locus_tag="VGC69_16420"
     CDS             10060..11736
                     /gene="yidC"
                     /locus_tag="VGC69_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY0296915.1"
                     /translation="MDIRRTVLWMIFSFSLLLLWNNWQVHSGKPSLFGSNTAATASTP
                     AASANGSSSAAIPNAPAAAAATPPSTVPSMAAATSAPSAQVVVTTDVLRLTFDTTGAQ
                     LVRAELLKYATDGQPTVLLDRSPTLTYVAQSGVVGAPANATGFPTHQTPFRLVSTQLE
                     LTGDTLPITFEAESGGLKVTKTFTLHRGQYDIEVRHDLSNTGTATLTPSLYLQLERDG
                     SDPADTSRFYHTFTGMAVYSEQDRFQKITFSDIEKGKAHYIKQTDNGWIGVVQHYFAS
                     AWVPPQGKPRTIDVLQVQPNLYATRTVESVGEVAPGSTASVDSHLWVGPQDQRAMAAV
                     APGLELVVDYGWLTIIAKPLFKLMTWLHSLLGNWGWTIVALTVLVKAVFFPLAAASYK
                     SMARMKRVAPRLQALKEKYGDDRQKLNGAMMELYRTEKINPLGGCLPMVVQIPVFISL
                     YWVLLASVEMRGAPWILWVHDLAVRDPFFILPAVMMGTMFLQIKLNPTPPDPVQAKVM
                     MIMPLVFGGMMFFFPAGLVLYWCVNNTLSILQQWTITRGLNKQLAKAANS"
     gene            11775..12674
                     /locus_tag="VGC69_16425"
     CDS             11775..12674
                     /locus_tag="VGC69_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296916.1"
                     /translation="MRGLDIPALEIFVAAVEEKSLSKAAERQNVVTSAASKRVAELER
                     NLNRTLLHRHGRGVEPTPAGTLLYQRAKAILRSVQLTEQAIEQYSANGQAKIRLASNP
                     STILQFLPPIMGRFLSSQPHISVDLLEAHSFDIPRNVAESTVDIGIYHADYPAPGVAS
                     FPFRQDRIGLVVPQGHPLSGRGELFLEEALDYDLLGYFPRHSLDQFLAYVGQSLSRPP
                     NIRLQVSNFETRCNMIREGLGIGLAPENIARNYLASMGLALLRLSDAWAERKFFVCVR
                     NMVDLNQATSSLLAALRESAPAF"
     gene            complement(12685..13701)
                     /locus_tag="VGC69_16430"
     CDS             complement(12685..13701)
                     /locus_tag="VGC69_16430"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0296917.1"
                     /translation="MMKQVHDRLVRRVGAFAAAMLAAVAVSSQAAHAAGDDAADYPSK
                     PITLLVGFTAGGPSDTAARILANRLSGKWHDARIIVENRPGANGAIAAAQMVQSPADG
                     YTLFLVTHSHVNLKWLYSKLAFDPAKDFQPVAELLTMPHALAVGPSVKERDYAALAAD
                     IKAKPDVYTAASSGNGSDPHLVLAEFMEKTGLKIRHIPYRGAAPEVVDMLSGRVDMTF
                     ETLGGVLKEIQQGKLRALAIGGTQRDPQLPDVPTFIEAGVKDFSPESWYGVMAPAGTP
                     MAIVDKINAAVNEVMNTPEGAKMLATLGALPVKSSVEDFTRLYHHDIEANGELIKSLN
                     IAID"
     gene            14018..15379
                     /locus_tag="VGC69_16435"
     CDS             14018..15379
                     /locus_tag="VGC69_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0296918.1"
                     /translation="MLLDTLSAYGARDTLANLPDEIMHHAKRALLDWLAALYPGTQVA
                     PCRQLLAAHRDELGHGAASLPGNGTTAFPATAAWINGSASHAVEFDDIFRDAVYHPGC
                     PVIAAALALAETRAASGRALLNAIVVGYEISTRIGAAIQPSHYRYFHTTGTVGSLGAA
                     AAAAALCAPGNALAMQHAIATAATLAAGLQQAFRSDAMSKALHAGHAAAVGVRAGQGA
                     AQGLTGVPDILEGEVGFGAALSQQPRWEIATQGLGHDYNITHITQKNHACCGHTFAAI
                     DAILALRAAHGLSPQHISRIHVATYKTALNVTGNFKPSTVFEAKFSLPYVICQALVHG
                     SVRLNAFEPCRLNDPVTRSLMEKVSLTEDPALTAGFPSMRAARVTLTLADGTSHEFFA
                     PYRKGDPEAPLSDAELDDKFEELAGPVLGTTRAQALREAVHRLDTLDDIRVLDLAAPI
                     LSR"
     gene            15403..17478
                     /locus_tag="VGC69_16440"
     CDS             15403..17478
                     /locus_tag="VGC69_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0296919.1"
                     /translation="MQNSISFAQALLAPRRIALVGASGDPRKNTARPLRFMRKHGFQG
                     RIYPINAGRGEILGEKAYPGLDALPEPVDHVFVMIPGAQVLAVLDACAQAGARVVTIY
                     SDGFGETGPEGQARQRELLDKARSLGLRLLGPNSIGLANLHAGSIISVNAAFEAESLL
                     PGDLSIVSQSGSMMGSLISRAAARGFGFAKSVSVGNECDITVGEIVDALVDDPLTKVI
                     LLFLETLRDAPTLAAALERARTANKPVVAYKLGQSEQGEVLAQSHTGAMAGNAAAMDA
                     FFRAHGVMRVRNLETLFEIAPLAMRYADHPPVAGRPARVAVITTTGGGAATVVDNLGL
                     RGLTAVAPPPAFVEHMAGRGLRIRETPVIDLTLAATGSQYQDLLEQLLRADWCDAVLS
                     VAGSSAQFHPELAVKPLVQADKPAGKPLAVFLSPEAPESLLRLQQAGIAAFRSPEACA
                     DALATLFAPPGSAPVATAPFTWPATLPSSGLLSEHEASSVFAALGVPVAASCLTASQT
                     LAHPLSYPVAAKVCSRDLPHKTDVGAVRVGIPSEAALREAAREMLANVAARAPAARVD
                     GILVQAMEGRLIELILGFRRDPLAGPTVILGAGGIAAEISPDFAIRLAPVSVGEAWKM
                     IEEVRQTRLVRGFRGLPRGDCDALARAIAAFSRLACVDGAQVDEAEINPLFVQAQGVV
                     AVDGLIRLA"
     gene            17530..18687
                     /locus_tag="VGC69_16445"
     CDS             17530..18687
                     /locus_tag="VGC69_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0296920.1"
                     /translation="MDFSLTPEQLDFESSVRRFAEKELRDGAVQRAHSDAYPWDIARK
                     MGEQGLLGITIGEADGGIGGTLMDAVIAIQTVAAVCPRSADVVQAGNFGAIRVLAEYG
                     SPLQKEKYLAALLAGKLLISVGMTEPEAGSAVTELKTSATRDGQGWRINGSKIFTTHG
                     PHADIILAYVRFGPGTQGIGSVLIETKAEGVKLGNRSAFMSGEEWVEIFFDNVYLPDD
                     MVVLGEGGFKKQIAGFNVERIGNTSRSVALGRYAYEEARQWAMQRKQFGRLLCDFQGL
                     QWKFADMRIKLDAAQLLLYRAASKADTGFPDATETAIAKAYCNQIGFDVANDALQVMG
                     GMGYSQESLVEYCVRRCRGWMIAGGSIEILKNRIAEGIFERAFPQRLPKPA"
     gene            18864..>20631
                     /gene="katG"
                     /locus_tag="VGC69_16450"
     CDS             18864..>20631
                     /gene="katG"
                     /locus_tag="VGC69_16450"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEY0296921.1"
                     /translation="MTDKTPEAKCPFNHAAGGGTTNKDWWPNQLHVELLSQHSNKTDP
                     LGEKFDYAEEFKKIDYEALKADLRKVMTNSQDWWPADFGHYGPQFIRMAWHAAGTYRI
                     ADGRGGGGRGQQRFAPLNSWPDNVNIDKSRRLLWPIKQKYGQKISWADLMQLTGNVAL
                     ETMGFRTFGFGAGREDVWEPDRDVNWGSETTWLGTDKRFTGDRDLDQPLSATVMGLIY
                     VNPEGPNASGDYLAAARDIRATFNRMAMNDEEIVALIAGGHTFGKTHGAAPESHKGPE
                     PEGAPLEAQGLGWISDYGSGHGKDTVSSGLEVTWTKTPALWSNNFLENLYAFEWELTQ
                     SPAGAKQWIAKDAPEIIPDAHIPGKFHKPTMLTTDLTLRFDTEFGKIAQRFLNNPQAF
                     AEAFARAWFKLTHRDMGPKIRYLGPEVPKEDLIWQDPLPKATLSPGAADIAEAKEKIA
                     AAGLSVSELVGVAWASASTFRGGDKRGGANGARIRLAPQRDWEVNKPVISALAKLEEI
                     QKASGKLSLADLIVLAGGVGIETAARAAGIAVTVPFTPGRVDASQDQTDLESFGYLEP
                     VADGFRNYTSKLAGVPAEHLLID"
BASE COUNT         3577 a         6971 c         6399 g         3684 t
ORIGIN      
        1 cgacatcgtt gttggcgaat gcaatcgcct gggccatgat cctgctccct catgcctgat
       61 gcatccatct gagcaatggg gcatttcagg ttcatgacgc ggcggcgggc attcacgatc
      121 ggtagcaaga aagccgctgc atgggtagcg gcgacctgtc cggaacccgg cgccgcccct
      181 aggtggagag tccggccgcc ggcctgcgga tcatcgccca gcccagcgcg tagaagcaaa
      241 gcccgccgat caccaggcag gccatcatgc aggcaaagcc gcttgcaaag gagtgggtgg
      301 actggaccag gtaacccacg atggacggcg ccagcagccc gccgatctgg ccgcccacgt
      361 tgacgaatcc ggccatcgtg gcccggccgg cgaccggcgc caggtcgagc atcagcgacg
      421 accagggcgc gaagctgccg tacaggcaca aggccaccgc cgacaagccc gccacgcagg
      481 cagccgcgct accggcgatg gaggccaggt agagggcgca ggcggccacg acgtaggccg
      541 aaccgagcaa cgccgcacgg tggcggtaga gccggcggtt gctcagccat ccgaacagca
      601 ccaggcccag gaagccgcag aagtagggaa tggacgccgc cacccccagg cccttgaggt
      661 cgatgtggcg ttcgatggag aggtagctgg gcagccaccc caaaaaggcc cagaagccca
      721 tattgaaacc catgaagccg aagaacagca gccagctggt cggccggctg gcaatcagcg
      781 ccagcagcgc gcgccagccc aggccggctg ccggcgtctc agtgtcctgc agcaccgtca
      841 ggcggcgctg gtcgggaacc gcgacgagca cgatcaaagc caccaccagg cccgcggcgg
      901 cgaaccacca gaacaattgc cgccaaccga ccaggcccaa cagccaggtg cccacgggcg
      961 cggccagtgc cgggcccagg gcaagcgcgg tgaaccacac cgcattggcg cccgcgcgtt
     1021 cacggaacga aaaattgtca ccgacgattt tgtaataagc cgaattggac gaaccctcgg
     1081 ccgcgccgaa gcagatgcgg accagtatca ggccggccac cgaggaaacc atgccggtca
     1141 tggccgtgaa aaaagaccag accagcggcg ccacgatcat cagcgccttg gcgccgaaac
     1201 ggtcggcaag gtagccgccc ggcagctgca tcagcagata gcccagcgtg aaggcgctga
     1261 ggatgaagcc gaacgatgtc gggcttatcc ccaaagaatg cattatcgtc ggacccgcaa
     1321 tggcgatatt gacgcgatcc atgtaattga ccaactgggt gatccagatg accgccagta
     1381 gctgatagcg cgaactcatc cgtgctcctc ctccgagttg acgggtaggc ctgatggccg
     1441 cgccgctggg tctcctggtt ttttggttga tcatggttgg cgcgccacgg catggcggcc
     1501 accggtgaaa acgtccaggt agacggcaaa ttaatgcaca atgcatctta tggctggcgg
     1561 aaacggcatg tcaagctacg gggagccggc catcagaccg cccgggacgg gtgaaacact
     1621 gccgcgtggt gcccaatccg ctgggccgcg caggaaaggg ggagcagcag gggtgcgggc
     1681 cggaatgggt gatcgagcgg cccgggcgat ggccgcgtga acacacgcta gaatttccca
     1741 tctccaggac gccaggtttg aggtaataca ttacatgttc gtggaccaga catcgcttct
     1801 gctcgccctc ggcattgcct cgttcgcgct gtcatgcacc ctgttggtca cttggctttt
     1861 cacccgcacc gatccgttca tcctgacctg ggccctcggc accttcgtcc tggtcttcgc
     1921 ctttgcctgt ttttcaatct atgcggtcaa tttcaacgca ctgctcggcc tggtctcgaa
     1981 cttcctgctg acctcgagct tcgtcatcct ctacggcgcg tcgtgcctgt tcaccgaaca
     2041 acgcctgccg atacggcgcg tggcgatcat cgaaacgagt tgcctgctgc ttctgggcct
     2101 gcccgtcgcg ttcggcttca ccgcgctcgg cgccctggcc ggcaacctgc tcaacgcggg
     2161 cctcctgttg ctgacggcgc gcaaattctg ggtcggccgc cacgaaacgc ccgtgtggat
     2221 cgtgggcatc gtgatctttt atacgctcgc cgccctgtcg ttcctcccct gcgcggccat
     2281 gatctatctc aaggggccgc tggtgctgag agccccgccc agcggctggg ccgaagatct
     2341 caattccatc gtcgggctca tcggcataac cggcatcggc gccctgtcgc tggcgctgaa
     2401 ccaggcgcgc agcgccagcc gccaccgcga ggaagcccgc accgatccgc tgacgggcct
     2461 gctcaaccgc cgcgccttgt tcgatcttta cggcgtcggc accctcgcaa agaacacggc
     2521 cgtgctggtg ttcgacctcg atcacttcaa gtccatcaac gacgtgcatg gccacggcat
     2581 gggcgacgaa gtgctgcgcc gcttcgcgcg cgtaatgcga gccagcttgc gcgcccgcga
     2641 taccgcggcc cgcctcggcg gcgaggaatt tgccctcatc ctgcccgcca gcacgcccga
     2701 aatcatcatg cgcctggccg aacgcatccg gatcggcttt gccgccgaga cgttccagac
     2761 gccgtcgggg ccgctgcgct gcacggtcag cgcgggcgtc gccttcgccg tggaagagag
     2821 cgagcatttc gacgccgtcc tctcgcgcgc cgacaaggcg ctttatctcg ccaaggacag
     2881 cggccgcaac cacgtcatgg cgcccgactt gaagcgggtg gcgtaggcgc gggccgcgca
     2941 aggcatcggc gcccaagcgc cgaaggctgg caattctttg gcccatgcct tccagcgcat
     3001 tatccgagcc gaagtagggg gtgtagccgg acatcttgca gctagcccag agtttcctgt
     3061 tccaattggc cctcagccac gccgtcccca gcttgattaa gctgttgagg gcggatatgg
     3121 tgccgcccaa ccgggcgtgc aaccaagtcg gccaactgca gccctgccaa attcgtcttc
     3181 ttatcggcaa agatgatatc gaacggcaga atgcggttgc cggggttggc gccatcgcaa
     3241 atacgccgaa actcaagttc cagttccgca tcttcctttt ttccccggca ctccacgatg
     3301 acatgcgttc taagacacag gcgatgagga tgaaggttag cctccataat ggaggtcaat
     3361 tgccccatga acgcatcgcg cgtgggcctg tggctcagaa aggcaaagtc gcccttctgc
     3421 ttgcgtatgt cgttttcgaa tggccgccgc tttcatcaac gtacacgatg aaatcactgt
     3481 attgccgacc cacactcgta ggcgagtcct tttcgaccgg aatggcctcg acggattcaa
     3541 acaacgggta ttgggcacca gagatgctca tgctacggtc tctgcggcat gcgactgggc
     3601 attgagtaag cggctgtcat ctgccgagtc agcaaagcgg cgctccaggg gaatgatgtc
     3661 ttttgtaatt atgctgcacc ttgcatttaa ccaatagccc cgcccaatca atagaaagtg
     3721 ccccatgagt ttctcaactt atggggcact ttttctcacc ttatgagttc acctgcgcca
     3781 gcgtcacgcg tcgatattgc tcgccagcaa ggcatgcgac tcgatgaacg cgcggcgcgg
     3841 ctccacgtcg tcgcccatca gcgtggtgaa gacctcgtcg gccgcgatgg cgtcttcgat
     3901 ctgcacgcgc agcaggcggc gcaccttggg gtccatggtg gtttcccaga gctgctcggg
     3961 gttcatttcg cccaggccct tgtagcgctg cttgctgatg ccgcgctcgg cttcggcgcg
     4021 cagccattgc atggcctcgc ggaagtcgct caccggctgt tccttgcgct tgtcgccttc
     4081 gccgcgcgcc accacggcgc ccgcgccgat caggcccagg aaggtgcggg ccgattcggc
     4141 cagcacggtg tagtcggcgc cacgcacgaa gtcggcatcg atcacgctcg tgcgcacatt
     4201 gccgtggtgc atgcgcttga cgagcagacg gtgcttctcg gcgacctcgt cgaattcggc
     4261 cacgacgtcc acgccgctgc cgacatcgct gcccgccagg gcccgcgcca ggcgctgggc
     4321 cgaggcggca gccgcgtcgg cgtcgtccag gttgatctcc acgccttcgg ccagggccga
     4381 cagcgccgcc acgtcgaaca cgcgcgccag gcgcgagatg accgtgtcgg ccatcacgta
     4441 tttcttggcc agctcggtca gcgcgtcgcc ggtgatgggc gcggcgtccg cggacggcac
     4501 cagcttggcg tctttcagcg ccagctgcat catgtactgg gcctcttcga cctcgtcctt
     4561 caggtagcgt tcgtcgcggc cgaccttgac cttgaagagc ggcggctggg cgatgtagat
     4621 gtggccgcgc gccaccagtt cgggcatctg gcggtacagc agcgtcagca gcagggtgcg
     4681 gatgtgcgcg ccgtccacgt ccgcgtcggt catgatgatg aggcggtggt agcgcagctt
     4741 gtcgatgttg aagtcggggc cgatgctggt gcccagcgcg gtgatcagcg tggcgatctg
     4801 ctcgctggac acgaggcggt cgaagcgcgc cttctccaca ttgagcacct tgccgcgcag
     4861 cggcaggatg gcctggaact tgcggtcgcg gccctgcttg gccgagccgc cggccgagtc
     4921 gccctcgacg atgtagagct cgcacagcgc cgggtctttt tcctggcagt cggccagctt
     4981 gccgggcagg ccggcgcctt ccagcacgct cttgcggcgc gtcatctcgc gcgccttgcg
     5041 ggcggcttcg cgcgcgcgcg cggcctcgac gatcttgccg cacagcgcct tggcatcggc
     5101 gggatgctcg agcagccagg tttcgagcgt gcgcgccacg gcgtcttcca ccgcggggcg
     5161 cacttcgctg gaaaccagct tgtccttggt ctggctgctg aacttgggtt cgggcacctt
     5221 caccgacagc acgcaggcca ggccttcgcg catgtcgtcg ccggtcgtct cgaccttggc
     5281 cttcttggcc agctcgttgt cggcgatgta tttgttcagg acgcgcgtca tggccgcgcg
     5341 caagcccgtc aggtgcgtgc cgccgtcgcg ctgcgggatg ttgttggtga agcacagcac
     5401 cgtttcggta tagctgtcgt tccactgcat ggcgatttcg acgcccacgt tcacgccgcc
     5461 ggcgttcgac tcgctggcga ccgcgaacac attggggtgc agcaccgtct tgctgcggtt
     5521 gatgtactcg acgaagccct tgaccccgcc cgagaaggcg aagttctctt ccttgccctg
     5581 gcgctggtcg atcaggcgga tcttgacgcc gttgttcagg aacgagagct cgcgcaggcg
     5641 cttggcgagg atctcgtagt ggtattcgat gttgttgaag atctcgctgt cggccaggta
     5701 gcgcacctcg gtgccgcgct tggcggtgct gccggtgacg gtcagggggg ccacgcgcgc
     5761 gccgcggtgg aattccatct ggtgcgtctg gccgtcgcgg tggatggtca gcttcagcca
     5821 ctcggacagc gcattcacgc aggacacgcc cacgccgtgc aggccgcccg agaccttgta
     5881 cgagttctgg tcgaacttgc cgccggcatg cagctcggtc atgacgattt cggcggcgct
     5941 gcggtggaat tcgtcatcct tgtggatggc ggtgggaatg ccgcggccgt tgtcggtgac
     6001 cgagatcgag ttgtcggtgt ggatggtgac gacgatgtcg tcgcagtaac ccgccagcgc
     6061 ttcgtcgatg gcattgtcga ccacttcgaa caccatgtgg tgcaggccgg tgccgtcgga
     6121 tgtgtcgccg atgtacatgc caggacgctt gcgcacggcc tccaggccct tgagcatctt
     6181 gatcgaatcg gcgccgtagc tgttctcgtt ggcggtgttc tgttgatcgg acatgttttt
     6241 ccttaaatgc gcatgggcat gacgacgtac ttgaactgct cgtcgtcggg cagcgtgatg
     6301 agcgccgagg cgttggcgtc gggcatcacc gaccagcgca cggtctcggc cttgaggttg
     6361 cccagcacat cgagcagata gctcacgttg aagcccacgt ccagcggttc atgcgcgtag
     6421 tcgatgtcga gctcttcctg cgcctcttcc tgctcggcgt tggtggagga gatcttcatc
     6481 acgttctgcg acagctgcag ccgcacgccc ttgaacttgt cggtggtgag gatggccgcg
     6541 cgctgcaggc tgccctgcag ggcctcgcgg ctcacgtcga aatggcgcgt gtaggtggtg
     6601 gggatcacgc gcgtgaagtc ggggaacttg ccctcgacca gcttggagac gaactccacg
     6661 tcgccgaagc ggaagcggat ctggcccggg gccacgtcca ccgccacggg ctcgtcggag
     6721 tcctcgagca ggcgctgcat ttccagcacg gtcttgcgcg gcacgatcac ctcgtgcttc
     6781 tcggcaatgc cgtcggccgc cgtggcgcag tgcgccaggc ggtggccgtc ggtggccacc
     6841 gagcgcacga agccgggctc gaacaccagc agcatgccgt tgaggtagta gcggatgtcc
     6901 tgctgcgcca tggcgaagtg caccatattg aacaggtggc gcagcgtgcg ctgcggcagg
     6961 gtgagcgaca cgttccactg ctcgggctgg gccacggtgg gaaactcggt ggccgccagg
     7021 gtctgcaggg cgaagcggct cttggccgat tgcaccgaaa gcttgttcga cgccagcccc
     7081 agcttcacct cgcccgtatc gggcagggcc ttgaggatgt ccaggagctt tctcgccgcc
     7141 accgtggtgg cctcgttctc ggggccgacg ccgaagtcgg catgcgtggt gatctgcact
     7201 tcgaggtcgg tggcgatgaa ggagaccttg ctgccttcct tgcgcatcag gatattggca
     7261 agaatgggca gcgtatgccg cctttcgacg atgcccgcca cggttgagag cggcttcaac
     7321 aatgcgtcgc gcgtggtttg tacgagttgc atggtcatcc ttttagagtt tgttccaata
     7381 cgtgcagggt atggttcagc tccgcttgct tggcgcgggc atcggaaatc ttgcggaccg
     7441 catgcaacac cgtggtgtga tcacggccgc cgaacaggtc cccgatctcg ggcaggcttt
     7501 tctgggtcag ctccttggcc aggtacatcg ccacctggcg gggcagcgca atattggccg
     7561 gccgccgttt cgaatacatg tcggcgacct tgatcttgta gaaatcggcc acggtcttct
     7621 ggatgttctc cacggtgacc tggccgctgg aaaccgaaag caggtctttc agcgcgtcct
     7681 tgctcaagtc cacggtaagc tcgtcgcggc cgtgaaaacg cgcataggct agcactttgc
     7741 gcagcgcccc ctcgagctcg cgcacattgc tgcgcaggtg cttggcgatg aagaacgcca
     7801 cctcttcggg catggtggcg ccctcggact cggccttgcg cagcaggatg gccacgcgca
     7861 tttccagctc gggcggctcg atggccaccg tcaggccctg gtcgaagcgc gaggtcaggc
     7921 ggctgtcgat gcccgccatt tccttgggat aggtgtcgct ggtgatgatg atctgcttgc
     7981 gctgggccac catcgcctcg aaggcgtaga agaactcttc ctgggtgcgc tgcttgcccg
     8041 agaagaactg gatgtcgtcg atcagcagca ggtccagcga atggtagtag cgcttgaagt
     8101 cgtcgaaggt cttgcgctgg taggccttta ccacgtcagc cacgtactgg tcggcgtgca
     8161 cgtagcgcac atgcgtcccc gtgccggccg ccaccatggc gttgccgatg gcgtggatga
     8221 ggtgcgtctt gcccaggccc acgccgccat acaggaagag cgggttgtag gaggtgccgg
     8281 ggttctcggc cacctgcagc gccgcggcgc gggccagctg gttggccttg cccgtgacga
     8341 aattctcgaa ggtgaggtcg gtgttcaggc gcgagcgctc gtagaccacg ccggcggcgt
     8401 cgctggcggc ggacggcgcc ggcacggggg acggagcgac agccgccgcg ggcgcggcca
     8461 tgccggccgg cctgggcatg gccgcgcctg cgcccacggt cgcagccaca ccggcaccca
     8521 cccccgcaac ggcgcgctgc ggcacggccc gcgacacggc ggcggcgggc agctcgaaag
     8581 acacctgcac cggccgcgcg taccactcgg aagccagcgc ttcgatctga tgggagaagt
     8641 tcttacgcac ccagtcgagc ttgaaacggt tcggggcggc cacgcgcagc aggccctgct
     8701 cctcgtcgaa ggccagcggg accagcggcc gtatccaggc gctgatttgt tgggggggga
     8761 gttcctgctc aagacgactg atgcaggtct gccagaattc gttcatgtca ctcttgttct
     8821 aaacctcgat tctaccgtga ccgagggggg ttatccacag gccggggctt ccctgatcct
     8881 tccgtccccc tctaactgtt tgacaatgtt gccctttctt gttgaaatag tgggcttttc
     8941 caaatcttca tgggttactc gggtcgggtc acccaaggat tacccaaaac tattacccaa
     9001 cgtctacacc ggggttctta agcaagggac ttgagttaag actcgaggct cggctggagc
     9061 ccccctttac ccccttgttc tttgtggaac gcacatgaaa cgcacttacc aaccttccgt
     9121 tacccgtcgc aagcgtaccc acggttttcg cgtgcgcatg aaaacccgcg ccggccgtgc
     9181 cgttctgaac gctcgccgcg ccaagggccg caagcgtctg gccgtctaag gcctgactgt
     9241 tcaagccccg ctgggctcca gagcccgggc ctgcaaagtc atgacacgcg ccacgctccc
     9301 ggcggaggcg cgactgcatc gcccctccga gtacgccgcc gcgctcaaag gcaggcgcct
     9361 cgcccgaggg gcgtttttca ttgtgagcgc cgcgccgctt gtcccggccg ccgccccggt
     9421 cgacatctcg gccgcccccc tggccagccc tccgccggag ccggccccgc cggcctgcgc
     9481 ccggctgggc ttggtgatcg ccaagcgcta cgcgccgcgc gccgtgaccc gcaacgcact
     9541 caagcgcgtc gtccgcgaag ccttccgggc ccagcggctg gcgctgcccg cgcgcgacta
     9601 cgtcgtgcgt ctgcaaggca aggtcgcgcc cgtctcgctt accgaactca agcgcgccgc
     9661 gcgcgccgaa gtggacgctc actttggacg gattgcgcga tgaccggccc ggcctcgaac
     9721 atgacccgcc tgatgacgcg cctcctgctg atcgcgccca tccggttcta ccgctttttt
     9781 ctcagtccct ggataggccg gcagtgccgc tatacgccca cctgttcggc ctacgccatc
     9841 gaagccatcg aacggcatgg cgcctggacc ggcctgtggc tggccgtgcg ccgcatcggc
     9901 cgttgccacc cgtgggcatc cggcggccac gacccggtgc cggatcatcc ggcgagccat
     9961 aaaggctgcc gccgacatca tccgaaaccg gactaacact aattaacgct aaactggcga
    10021 gctttgctgg cttgttgccc gcagctaccc tctggaacca tggatatccg ccgaactgtc
    10081 ctctggatga ttttttcgtt ctccctgttg ctcctgtgga acaactggca ggtccatagc
    10141 ggcaagccgt cgctgttcgg atcgaacacc gccgccacgg caagcacgcc cgccgcgtcg
    10201 gcaaacggct cgtccagcgc cgccatcccc aacgcgccgg ctgccgcggc ggccacgccg
    10261 cccagcaccg tgcccagcat ggccgccgcc acctcggccc cgtcggccca ggtcgtcgtc
    10321 accaccgacg tgctgcgcct gaccttcgac accaccggcg cccagctggt gcgcgccgag
    10381 ctgctcaagt acgccaccga cggccagccc accgtgctgc tcgaccgctc gcccacgctg
    10441 acctacgtgg cgcagtcggg cgtggtcggc gcgccggcca acgccacggg ctttcccacg
    10501 caccagacgc cgttccgcct ggtctcgacc cagctcgagc tcaccggcga cacgctgccc
    10561 attaccttcg aagccgagtc cggcggcctg aaggtcacca agaccttcac cctgcaccgc
    10621 ggccagtacg acatcgaggt gcgccacgac ctgtccaaca ccggcaccgc caccctgacg
    10681 ccctcgctgt acctgcagct cgagcgcgac ggcagcgacc cggccgacac ctcgcgcttc
    10741 tatcacacct tcaccggcat ggccgtgtac tccgagcagg atcgcttcca gaagatcacc
    10801 ttctcggaca tcgagaaggg caaggcccac tacatcaagc agaccgacaa cggctggatc
    10861 ggcgtggtgc agcactactt cgccagcgcc tgggtgccgc cgcaaggcaa gccgcgcacc
    10921 atcgacgtgc tgcaggtgca gcccaacctg tacgcgaccc gcacggtcga gtcggtgggc
    10981 gaggtcgccc ccggctcgac ggccagcgtc gactcgcacc tgtgggtcgg tccgcaggac
    11041 caaagggcca tggcggccgt cgcccccggc ctggagctgg tggtcgacta cggctggctc
    11101 accatcatcg ccaagccgct cttcaagctg atgacctggc tgcacagcct gctgggcaac
    11161 tggggctgga ccatcgtcgc cctgaccgtg ctggtcaagg ccgtgttctt cccgctggcc
    11221 gccgccagct acaaatcgat ggcccgcatg aagcgggtcg ccccgcgcct gcaggccctg
    11281 aaagagaagt acggcgacga ccgccagaaa ctcaacgggg ccatgatgga gctctaccgc
    11341 accgagaaga tcaacccgct gggcggctgc ctgcccatgg tggtgcagat ccccgtgttc
    11401 atctcgctgt actgggtgct gctggccagc gtggaaatgc gcggcgcgcc gtggatcctg
    11461 tgggtgcacg acctggccgt gcgcgatccc ttcttcatcc tgcccgcggt gatgatgggc
    11521 accatgttcc tgcagatcaa gctcaatccc acgccgcccg accccgtgca ggccaaggtc
    11581 atgatgatca tgccgctggt gttcggcggg atgatgtttt tcttcccggc cggcctggtg
    11641 ctgtactggt gcgtgaacaa cacgctgtcc atcctgcagc agtggacgat cacgcgcggc
    11701 ttgaacaagc agctggcgaa ggcggccaac agctgaaaaa gcgctataac ctctgttaaa
    11761 acagaggagc gttaatgagg ggactagata ttccggcgtt ggagattttc gtcgccgcag
    11821 tagaagaaaa aagcctgtcc aaagctgccg agcgccagaa cgtggtgacc tcggcagcca
    11881 gcaagcgggt tgcggaactg gaacgcaacc tgaaccgtac cctgctgcac cggcacggcc
    11941 ggggcgtcga gcccacgccg gccggcacgc tgttgtacca gcgtgccaag gccatcctgc
    12001 gcagcgtgca gttgaccgaa caggccatcg agcagtattc ggcgaatgga caagccaaga
    12061 tccgcctggc gtccaacccc tcgaccatcc tgcagttcct gccgcccatc atggggcgct
    12121 ttctctcgag ccaaccccac atcagcgtag acctgctcga agcccacagc ttcgacattc
    12181 cccgcaacgt agccgaaagc accgtggaca tcggcatcta ccatgccgac tatcctgcac
    12241 ccggcgtggc ctcgtttccc ttccggcagg accgcatagg cctggtcgtg ccacaggggc
    12301 acccgctctc gggacgcggc gagctgttcc tcgaggaagc gctcgactac gacctgctgg
    12361 ggtattttcc ccgccattcc ctggaccagt tcctggccta cgtcgggcaa tcgctgtcga
    12421 ggccgccgaa catacgcctg caggtctcca acttcgagac ccggtgcaac atgatccggg
    12481 agggcctggg catcggcctg gcgcccgaga acatcgcccg gaactatctg gccagcatgg
    12541 gcctggccct gctgcgcctg agcgatgcct gggccgagcg gaaatttttc gtgtgcgtac
    12601 gcaacatggt cgacctcaac caggccacca gcagcctgct ggcagccttg cgggagtcgg
    12661 cccccgcgtt ctgatcctgc cccgtcaatc gatggcgatg ttcagcgact tgatgagctc
    12721 gccattggct tcgatgtcgt gatggtaaag ccgggtgaaa tcctccaccg aggatttcac
    12781 cggcagcgcg cccagcgtcg ccagcatttt tgcgccttcc ggcgtgttca tgacctcgtt
    12841 gaccgcggcg ttgatcttgt cgacgatggc catgggcgtg ccggccggcg ccattacgcc
    12901 gtaccaggac tcgggcgaaa aatccttgac gcccgcttcg atgaaggtcg gcacgtcggg
    12961 cagctgtgga tcgcgctgcg tgccgccgat cgccagggcg cgcagcttgc cctgctggat
    13021 ttccttcaag accccgccca acgtctcgaa tgtcatgtcg acccggccgc tcagcatgtc
    13081 caccacttcg ggcgcggcgc cgcgataagg aatatggcgg atcttcaggc cggtcttctc
    13141 catgaactcg gccagcacca ggtggggatc ggaaccgttg cccgaggatg ccgccgtgta
    13201 cacatcgggc ttggccttga tatcggccgc cagcgccgcg taatcacgct ctttcacgga
    13261 agggccgacc gccagcgcat ggggcatggt cagcaattcg gccaccggct ggaaatcctt
    13321 ggccggatcg aacgcgagtt tggaatacag ccacttcaga ttgacgtggc tatgcgtgac
    13381 caggaacagc gtatagccgt cggctggcga ttggaccatc tgggccgcgg cgatcgcgcc
    13441 gttggcccca ggccggttct cgacaatgat gcgcgcatca tgccacttgc ccgacaagcg
    13501 gttggccaga atgcgcgcgg ccgtgtcgga agggcctccg gccgtaaatc ccacgagcag
    13561 ggttatcggt ttggaagggt agtcggccgc gtcgtcaccg gccgcgtgcg cggcctggct
    13621 cgacaccgcg acggcggcca gcatcgccgc cgcaaacgcg cctacgcggc gcacgagccg
    13681 atcatggact tgcttcatca tgtttgtctc catatttata ggaagaatcg tgttcgagcc
    13741 ttccgagcct gcgctctcga aatcccttgt catgccttcg catgggattc ccgccgcggg
    13801 tcgcctggtt tttcttattg ctcgaaatct tacggaggcg gccaagcccg ggttatgaaa
    13861 aattggcaag gcaacgttct ccgttttcgt tcgcttgccg tgaatggaat gctggctttc
    13921 ctttttttca agccatgcgc acccgggagt cattagatta gggaggcacg atgatcaagc
    13981 gatgccttgg cgcatcgcaa tatggagaca acaccctatg ctgctcgaca cgctctcggc
    14041 ctatggcgcc cgggacacgc tggccaattt gcccgacgaa atcatgcacc atgcgaagcg
    14101 ggccctgctc gactggctgg ctgcgctcta tcccggcacg caagtcgcgc cgtgccggca
    14161 attgctggcg gcccaccgcg acgaactcgg ccacggcgcg gccagcctgc ccggcaatgg
    14221 aacaaccgcc ttccccgcga cagcggcatg gatcaacggc agtgcctcgc atgccgtgga
    14281 gttcgacgat atctttcgcg acgccgtcta ccatccggga tgcccggtca tcgccgcggc
    14341 gctggccctg gccgaaacgc gcgcggccag cggacgcgcg ctgctcaacg ccattgtggt
    14401 gggatatgaa atctccaccc gcatcggcgc ggccatccaa ccctctcatt accgttattt
    14461 ccataccacg ggtaccgtag gcagcctggg cgcggccgcc gcggccgccg cgttgtgcgc
    14521 gcccggaaac gccctcgcca tgcagcacgc catcgccacg gcggccacgc ttgccgccgg
    14581 cctgcagcag gccttccggt ccgatgccat gagcaaggcg ctacacgccg gccatgccgc
    14641 ggccgtcggc gtgcgggcgg gacaaggcgc cgcgcaggga ttgacgggcg tgcccgacat
    14701 cctggagggc gaggtcggct tcggcgccgc gctctcgcag cagcctcgct gggaaatcgc
    14761 cacccaggga ttgggacacg actacaacat cacgcacatc acccagaaga accatgcctg
    14821 ctgcggccat accttcgccg ccatcgacgc catcctggcc ttgcgggcag cgcatggcct
    14881 gtcgccgcag catatctccc gcatccacgt cgccacttat aagaccgcgc tcaacgtcac
    14941 gggcaatttc aagccttcta ccgttttcga agccaagttc agcctgcctt atgtgatttg
    15001 ccaagccttg gtgcatggct cggtgcggct caatgcgttc gagccctgcc ggttgaacga
    15061 cccggtcacg cgttcgctca tggaaaaggt ctccctgacc gaggatccgg cgttgactgc
    15121 cgggttcccc tccatgcgcg ccgcacgcgt cacgctcacc ctggccgacg gcacctccca
    15181 cgaattcttc gccccctacc gcaaaggcga tcccgaagcc cccttgtccg atgccgagct
    15241 ggacgacaag ttcgaagagc ttgccggccc cgtcctcggc accacgcgag cccaggcgct
    15301 gcgagaagcc gtccatcggc tcgacacgct cgacgacatt cgcgtcctcg atctggccgc
    15361 ccccatcttg agccgctgag cttgcaaccc atcacgacgt ttatgcagaa ctccatttct
    15421 ttcgcccaag ccttgcttgc tccgcgacgc atcgccctgg tcggcgcctc cggcgatccg
    15481 cgcaaaaaca cggcccggcc cttgcgcttc atgcgcaaac acggtttcca ggggcgcatc
    15541 tatcccatca atgccggccg cggtgaaatt ctcggggaga aggcctaccc cggcctggat
    15601 gcgctaccgg aaccggtaga ccatgtcttc gtcatgatcc ccggcgcgca ggtgctggcc
    15661 gtgctcgatg cttgcgcgca agccggcgcc cgggtcgtca ccatctactc cgacggcttc
    15721 ggcgagaccg gccccgaagg acaggcccgc caacgcgagc tgctcgacaa ggccaggagc
    15781 ctcggcctgc gcctgctcgg ccccaatagc atcggcctgg ccaatctgca cgccggcagc
    15841 attatttccg tcaacgccgc cttcgaagcg gaatccctgc tgcccggcga cctcagcatc
    15901 gtgtcgcaga gcggctcgat gatgggttcg ctcatttcgc gggcggccgc gcgcggcttt
    15961 ggcttcgcca agtctgtttc cgtcggcaat gaatgcgaca tcacggtggg cgagatcgtc
    16021 gacgccctgg ttgacgatcc gctcaccaag gtcatattgc tgttcctcga aaccctgcgc
    16081 gacgcgccga ctctggccgc tgcgctggaa cgcgcgcgca cggccaacaa gcccgtggtc
    16141 gcctacaagc tgggacaatc cgagcaaggc gaggttctgg cccaatcgca caccggcgcc
    16201 atggccggca acgccgccgc catggacgct tttttccgcg cgcatggcgt gatgcgcgtg
    16261 cgcaatctcg aaacattgtt cgagatcgcg ccgctggcca tgcgctacgc ggatcatccg
    16321 cccgtggccg gccggcccgc aagggtggcc gtcatcacga caaccggcgg cggggccgcc
    16381 acggtcgtcg acaatctcgg actgcgcggc ctgaccgccg tcgcgccgcc gcccgccttc
    16441 gtcgagcaca tggccggacg cgggctacgc attcgtgaaa cacccgtcat cgatctgacc
    16501 ctggccgcga cgggcagcca gtaccaggac ctgctggaac aactgctgcg cgccgactgg
    16561 tgcgacgccg tgttgagcgt agcgggatcc tcagcccagt tccaccccga actggccgtc
    16621 aagccgctgg tccaagccga caagcccgcg ggcaaaccgt tggcggtatt cctgtcgccc
    16681 gaagcgccgg aatccctgct gcgcctgcag caggccggca tcgccgcctt ccgcagcccg
    16741 gaagcctgcg ccgacgcgct ggcaacgctc tttgcgccgc ccgggtcggc acccgtggcc
    16801 accgcgccct ttacctggcc cgccacgctc ccatccagcg gcctgctgtc cgagcatgag
    16861 gcaagttcgg tgttcgccgc gctgggcgtg cctgtggcag cctcgtgcct gacagcctcc
    16921 cagaccctcg ctcatccgct ttcctatccg gtggcagcca aggtgtgctc gcgcgacctg
    16981 ccccacaaga ccgatgtcgg cgcggtacgg gtcggcatcc ccagcgaggc ggccctgcgg
    17041 gaagccgccc gggagatgtt ggccaatgtc gcggcgcgcg caccagcggc acgcgtcgat
    17101 ggcatcctcg tgcaggccat ggaaggccgc ctgatcgaac tcattctggg tttccggcga
    17161 gacccgctgg ccggtcccac cgtcatcctc ggtgcgggcg gtatcgccgc ggagatctcg
    17221 cccgacttcg ccatacgcct ggcccccgtc agcgtcggcg aagcctggaa gatgatcgaa
    17281 gaagtccggc agactcgcct cgtccgcggc tttcgcggcc tgccgcgcgg cgattgcgat
    17341 gccttggccc gcgccatcgc ggcgttctcg cgcctggcct gcgtggacgg ggcgcaagtg
    17401 gacgaagccg agatcaatcc cctattcgtc caggcacaag gcgtggtcgc cgtggatgga
    17461 ctgatccgac tggcctgaaa acacctcgac aagaacctat cgtttgcttt gcataggaag
    17521 tcattcaaca tggatttttc cctgacaccc gagcagctgg atttcgaatc gtccgtacgc
    17581 cgcttcgccg aaaaagaact gcgcgatggc gccgtccagc gcgcgcatag cgacgcctat
    17641 ccctgggaca tcgcccggaa aatgggcgag caaggcttgc tgggcattac gatcggcgag
    17701 gcggacggcg gcatcggcgg cacgctcatg gatgccgtca tcgccatcca gaccgtcgct
    17761 gcggtctgcc cgcgcagcgc ggacgtcgtg caggcgggca atttcggcgc aatacgcgtg
    17821 ctggccgaat acggcagccc tctccagaag gagaagtacc ttgccgcctt gctggccggc
    17881 aaactgctga tttcggtcgg catgaccgag cctgaggccg gctcggcggt gaccgaactg
    17941 aaaaccagcg cgacgcgcga cggccagggc tggcgcatca atggttcgaa aattttcacc
    18001 actcacggtc cgcatgccga cattatcctg gcctatgtcc gcttcggtcc cggcacgcag
    18061 ggcatcggtt cggtactgat cgaaaccaag gccgagggtg tcaagctggg caatcgctcc
    18121 gctttcatgt ccggggagga atgggtcgag attttcttcg acaacgtcta cctcccggac
    18181 gacatggtgg tgctaggcga aggcggcttc aaaaagcaga ttgccggctt caatgtcgag
    18241 cgcatcggca atacgtcccg atcggtggca ttggggcgct acgcttacga agaagcgcgc
    18301 caatgggcca tgcaacgcaa gcaattcggc cgcttgctct gtgatttcca aggcctgcaa
    18361 tggaagttcg cggacatgcg gataaagctc gacgccgcgc agttgctgct ctaccgcgcc
    18421 gcatccaagg cggacaccgg ctttcccgac gcgaccgaaa ccgccatcgc caaggcctac
    18481 tgcaaccaga tcggattcga cgtcgcaaac gatgccttgc aagtcatggg cggcatgggg
    18541 tacagccagg aatccctggt ggaatactgc gtgcgccgct gccgcgggtg gatgatcgcc
    18601 gggggttcga tcgaaatcct gaagaaccgc attgccgaag ggatattcga gcgcgccttc
    18661 ccgcaacggt tgccgaaacc ggcctgagtc tcctttcatg cgccgatacg cccgcggctt
    18721 ttccaggagc cgcgggcgtt agttttttta ataccgcgcc atcggggaaa tctatttctt
    18781 tttgtaattg agagccctta tcatgggtca acttcccctt gccgcaggcg catgcccggc
    18841 tctcatttca gatggagcaa gtgatgaccg acaagacacc cgaggccaag tgccccttca
    18901 accacgccgc gggcggcggc acgacgaaca aggactggtg gcccaatcag ctgcacgtcg
    18961 aactgctgag ccagcattcg aacaagaccg acccgctggg cgagaagttc gactatgccg
    19021 aagaattcaa gaagatcgac tatgaagcgc tcaaggccga tctgcgcaag gtgatgacca
    19081 actcgcagga ctggtggccg gccgacttcg gccactacgg accgcagttc atccgcatgg
    19141 cctggcacgc cgccggcacc taccgcatcg cggacggccg cggcggcggc ggccgcggcc
    19201 agcagcgttt cgcgcccttg aactcctggc ccgacaacgt caacatcgac aagtcgcgcc
    19261 gcctgctgtg gcccatcaag caaaaatacg gccagaaaat ctcctgggcc gaccttatgc
    19321 agctcaccgg caacgtcgcg ctggaaacca tgggctttcg caccttcggt ttcggcgccg
    19381 gccgcgaaga cgtctgggaa cccgatcgcg acgtcaactg gggcagcgaa accacctggc
    19441 tgggcaccga caagcgcttc accggcgacc gcgacctcga ccagccgctg tcggccaccg
    19501 tcatgggcct gatctatgtc aaccccgaag ggcccaatgc cagcggcgat tatcttgccg
    19561 ccgccaggga catccgcgcg accttcaacc gcatggcgat gaacgacgag gaaatcgtcg
    19621 cgctgatcgc cggcggccac accttcggca agacgcacgg cgccgccccc gaatcgcaca
    19681 agggccccga gcccgaaggc gctccgctcg aggcccaggg cctgggctgg atcagcgact
    19741 acggctccgg ccatggcaag gacacggttt ccagcggcct ggaagtcacc tggaccaaga
    19801 cgccggccct gtggagcaac aacttcctcg aaaacctgta tgccttcgaa tgggagctga
    19861 cccagtcccc cgccggcgcc aagcagtgga tcgccaagga cgccccggaa atcatccccg
    19921 acgcgcacat ccccggcaag ttccacaagc ccacgatgct gacgacggac ctgacgctgc
    19981 gcttcgatac ggaattcggc aagatcgccc agcgcttcct gaacaacccg caagcgttcg
    20041 ccgaagcctt tgcccgcgcc tggttcaagc tgacccaccg cgacatgggt ccgaagatcc
    20101 gctacctcgg cccggaagtg ccgaaggaag acctgatctg gcaagatccg ctgcccaagg
    20161 ccacgctcag ccccggcgcg gcggacatcg ccgaagccaa ggaaaagatc gcggccgccg
    20221 gcctgtcggt gtccgaactg gtcggcgtgg cctgggcctc ggcgtcgacc ttccgcggcg
    20281 gcgacaagcg cggcggcgcc aacggcgcgc gcatccgcct cgcgccgcag cgcgactggg
    20341 aagtcaacaa gcccgtcatc tccgccctgg ccaaactgga agagatccag aaggcctcgg
    20401 gcaagctgtc gctggccgac ctcatcgtgc tggccggcgg cgtgggcatc gagacggcgg
    20461 ccagggccgc gggcattgcc gtcacggtgc cgttcacccc gggccgcgtc gatgcctcgc
    20521 aggaccagac cgacctcgag tccttcggct acctcgaacc ggtggccgat ggtttccgca
    20581 actacacgtc caagctcgcc ggcgttcccg ccgaacacct gctcatcgat c
//
LOCUS       DASWPO010000315         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2843442, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000315 DASWPO010000000
VERSION     DASWPO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843442"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VGC69_16455"
     CDS             complement(<1..168)
                     /locus_tag="VGC69_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014322.1"
                     /note="SodA; manganese binding; only present under aerobic
                     conditions; destroys free radicals; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HEY0296922.1"
                     /translation="MSYALPPLPYAYDALEPHIDAQTMEIHYSKHHQTYVNNINATLE
                     TAGLEFLPIDEL"
     gene            complement(254..724)
                     /locus_tag="VGC69_16460"
     CDS             complement(254..724)
                     /locus_tag="VGC69_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296923.1"
                     /translation="MHAAFHHEVKTADSPAGLEGWRSAIARGNLAFKLADYAGAIAQY
                     RAAHSWADALFGHIDDVDAGVAAFVVSCHNLADTYECLDRPDEQESCLCLAHEKLCAA
                     MNDGSLAEPWRLAALRHSRRTYAELLRFLGRHPGHARARAGCALGAAGPTPLAH"
     gene            1207..>1999
                     /locus_tag="VGC69_16465"
     CDS             1207..>1999
                     /locus_tag="VGC69_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0296924.1"
                     /translation="MESAWNSAVALMPRLLDLGLNLLVAVLILVIGWWISSMLGNWVR
                     RAAERSGRVDRTIIPMLRATMVWAVRIFTLIAVLARFGVQTTSLIAVLGAAGLAVGLA
                     LQGTLQNIAAGIMLLILRPIRAGESASLSSGTAGMVDEIGLFLTRIVQDDGIVVLLPN
                     STVWGATITNFSRNTRRRLDLPVIVNYGDDLGAAIELVRKVVTAHPMAEASPEPIVKA
                     SEYKDSGTLLNVRVWSQNANYWDLRWDLFHQIRTAMDAAGFKPPAQ"
BASE COUNT          354 a          599 c          660 g          386 t
ORIGIN      
        1 cagttcatcg atggggagaa actcgagccc ggctgtttcc agcgtggcgt tgatgttgtt
       61 gacgtaggtc tgatggtgct tgctgtagtg gatctccatg gtctgcgcgt cgatatgcgg
      121 ttcgagcgca tcgtaggcgt agggcagggg agggagggca taagacatgg agaactccga
      181 aagtaagctc gtgtgagcga aaggcggaat cgtggaacac tcagcgccgg cggcgcggca
      241 tggcaagtgc atgtcaatgg gcaagcggcg tcgggcccgc cgcgcccagg gcgcatccgg
      301 cgcgagcccg tgcatgcccg ggatggcggc ccagaaaacg cagcaactcg gcataggtgc
      361 gccggctgtg gcgcaatgcc gccaggcgcc agggttcggc cagactcccg tcattcatcg
      421 ccgcgcagag cttttcatgt gccaagcaca ggcaggactc ttgttcgtcg ggacgatcca
      481 ggcactcgta tgtgtcggcc aggttgtggc aagacacgac gaaggccgcc acgccggcgt
      541 ccacgtcgtc gatatgaccg aatagcgcat cggcccagga atgcgcggcg cggtactggg
      601 cgatggcacc cgcatagtcg gccagtttga atgcgagatt gccgcgtgca atggcggatc
      661 gccagccttc taggcctgca ggcgagtcgg ccgttttgac ttcgtgatgg aatgcggcgt
      721 gcatggggcg ttctccgtaa gcaggtcgtg aacgatctta ttataaatga taatcatttt
      781 catctttggg tgtaagtatt ttgacaaaac gagggcctgc cgcgcttgcc tggaatcccg
      841 ggcaaccctg cgggccgttc aatacggcat cggcccggca ggacaaaatt tgcgcagcac
      901 cggcaagtgc ttgtcgcaaa aatgattttt cctcatgccc ggatgctgtc ctcagcctta
      961 tccacatggg ctggggaaaa cttcggcacg gtgtgccctt tttttgcgcg cctcgatcaa
     1021 gatattgatt cgcatggcaa aaagccggct gccccgaggc tgtcctcagc gttgtccaca
     1081 tgaggcgtgg ataacctccc ggatggccgc cgcatcatgc ttacgcagtg taggagtgcg
     1141 atgccgcgtc atctgctaga gttgccgcgt ccgccgcgcc gcgcgcggca cgagttgaga
     1201 gacgacatgg agtctgcatg gaacagcgcg gttgccctga tgccgcgcct gcttgatctg
     1261 ggattgaacc tgctggtggc ggtgctgata ctggtcatcg gctggtggat atcgtcgatg
     1321 ctgggcaact gggtgcgccg ggccgccgag cgttccggcc gggtcgaccg caccatcatt
     1381 cccatgctcc gggccaccat ggtctgggcg gtccgcatct ttaccctgat cgcggtgctg
     1441 gcgcgcttcg gcgtgcagac caccagcctc atcgccgtgc tgggcgccgc cggcctggcc
     1501 gtgggcctgg ccctgcaggg cactttgcag aacatcgcgg ccggcatcat gttgctgatc
     1561 ctgcggccga tccgcgccgg cgagtcggcc tcgctgagtt cgggcaccgc cggcatggtg
     1621 gacgagatcg gcctgttcct cacgcgcatc gtgcaggacg acggcatcgt cgtcctgctg
     1681 cccaacagca cggtctgggg cgccaccatc accaacttca gccgcaacac caggcgccgt
     1741 ctcgatctgc cggtgattgt caattatggt gatgacctgg gcgcggccat cgagctggtg
     1801 cgcaaggtgg tgaccgcgca tcccatggcc gaagccagcc ccgaacccat cgtcaaggcc
     1861 agcgagtaca aggacagcgg cacgctgctc aacgtgcggg tctggagcca gaacgcgaat
     1921 tattgggacc tgcgctggga cctgttccac cagatccgca cggccatgga cgcggcgggc
     1981 ttcaagccgc cggcccagg
//
LOCUS       DASWPO010000316         8431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1505447, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000316 DASWPO010000000
VERSION     DASWPO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505447"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VGC69_16470"
     CDS             <1..752
                     /locus_tag="VGC69_16470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0296925.1"
                     /translation="AVPARYLAQTAGAGDMIGICIPAHNEEAYIGKCLRSVMHAARHP
                     LLNGEPVEIVVVLDTCSDETEMHARRWPVTCLHTALRNVGFARAAGARHLLAAGARWL
                     AFTDADTVVSQSWLVDQLSLDSDVVCGTIDVSDWDEHGVYETLTRAFFLSYYKDEDGH
                     RHVHGANLGIDAMAYQSVGGFESLACSEDREIVERLERAGASIAWTALPRVTTSARPF
                     SRVAGGFATALRQAWPEDGQALFPDVPPSPT"
     gene            764..3157
                     /locus_tag="VGC69_16475"
     CDS             764..3157
                     /locus_tag="VGC69_16475"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0296926.1"
                     /translation="MATRKRQPTKMPQQPALDTAYFNSDSAPALPPAPAASQPALKQA
                     RAVGETVDGVPANSNKAAEYGEKAARPPVGATVEPPDESVTASTLSEKNRSAKTGAKA
                     SVGVNAASGELPRVRADSGGQAMTTNQGVPVADNQSSLKAGLRGPALLKDFILREKIT
                     HFDHERIPERIVHARGSAAHGYFECYEPLTDLTCASLFSEAGKRTPVFVRFSTVAGER
                     GSKDTARDVRGFAVKFYTDQGNWDLVGNNIPVFFIQDAMKFPDLVHAVKPEPHHGMPQ
                     AASAHDTFWDFISLMPESIHTIMWVMSDRGIPRSYRMMQGFGVHTFRFVNAAGAAKLV
                     KFHWNPKLGTHSHVWDEAVKISGADPDFHRRDLWEAIEAGAYPEWELGVQIVDEAQAD
                     KFSFDVLDATKLIPEELVPVKPIGRLVLNRNPDNFFAETEQVAFCTAHVVPGVDFTND
                     PLLAGRIHSYVDTQISRLGGPNFHELPINAPLAPVHNNQRDGMHRQAIHRGRVAYEPN
                     SLAGGCPFQAGTMGFLPFPEPVAADELRGKPEKFAEHYNQAALFYESQAGHEQQHIIE
                     AFSFELSKVTVPGIRRRTVAMLRNVSEDLANAVAANLGMPELPQPLPRAAKTNTKPEI
                     ATSEFLSLLARPGDGGIRSRKVAIMAADGVDAQAAQQVAQALIAKGAVVRLVGQHVGL
                     MAGAGGETMDADASFKNSPSPLFDAVVVPDGAQAIDALCADGVALEFVRDAFRHGKTL
                     MAIGEGPRLLQHAGIPVDDADPGLVLAAAAEKSAIAAFINALGKHRHPARESDPPVV"
     gene            3230..3691
                     /locus_tag="VGC69_16480"
     CDS             3230..3691
                     /locus_tag="VGC69_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY0296927.1"
                     /translation="MSANNIYEALRESHAVQRSLCRKLLRAAPHSERRREIYRELKIE
                     LAAHAAAEERFLYAPILMDDMGLDSSRHALSEHHEIDEYVEELGKADAQGGSWLAKAR
                     KLSDEVHHHLREEEKKFFQVSGKILTETQKEKLARQYLKDYQRMKTRLAQS"
     gene            3785..4603
                     /locus_tag="VGC69_16485"
     CDS             3785..4603
                     /locus_tag="VGC69_16485"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEY0296928.1"
                     /translation="MSRKQDCKDEYKTADELSHTLPSKAVAVHEIIRKEGEKELSRDF
                     LALFWSAIAAGLTMSTSMVGRGAMQAYLPDERWAFLIEAAGYTLGFIFVITAGQQLFT
                     ENTVTPVLPFMSRPSIRQLARLLRLWGIVLLGNLIGGLIAAAVFAWLPMFPPEIVEAF
                     RKIGRHLLETPILTNFETAILAGWLIATLVWMLRAVENGKIALIFLVTYLMGIAGVAH
                     VVVGTIEVGYLVLLGEASVADALSRSIIPTLVGNVIGGTFIFALISHAQVRADI"
     gene            complement(4646..4912)
                     /locus_tag="VGC69_16490"
     CDS             complement(4646..4912)
                     /locus_tag="VGC69_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296929.1"
                     /translation="MSTEKAVEKAKDLAEKGEDIATGVIDSAASEAKRRVRSAKGAVD
                     EALDDGRDALENAMVCAKDMIRQHPFTAVAVVGAIAYLWGKIRG"
     gene            complement(5009..5674)
                     /gene="pdeM"
                     /locus_tag="VGC69_16495"
     CDS             complement(5009..5674)
                     /gene="pdeM"
                     /locus_tag="VGC69_16495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY0296930.1"
                     /translation="MPGAHEGSLAVRLAGEPVVLLGERALYWPARRRLVIADLHLGKG
                     HVFRRAGIAVPSGATQDDLARLAGLVAATAAHELWIVGDVLHGPASQASWRAAWTQWR
                     RAHARLDVAALAGNHDRALDGAALGLRQLGEVQADGPFVFRHLPQADAQDRHVIAGHV
                     HPKTRLPGVPRAWPVFWLRPAMTVLPAFSAFTGGRLVEAGLGDTLAACVEGTVIAIPA
                     SRA"
     gene            complement(5658..8120)
                     /locus_tag="VGC69_16500"
     CDS             complement(5658..8120)
                     /locus_tag="VGC69_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEY0296931.1"
                     /translation="MKAGPRGWDGPLAAWFVARGWKAARFQREVWRRYLDGESGLLHT
                     PTGSGKTLAAFGGPVLQALADPAAARTAAGDGPRVLWVTPLRALAADTAQALAQVAQD
                     LGLGWTVALRTGDASARDKRLARQGKADVLVITPESLALLLSYADASSRFRALRCVVV
                     DEWHELLGNKRGVLLQLCLARLRRLSPGLRTWGLSATLGNLQEARAVLLPHSPDSPLV
                     SGAPARKVTLSTLLLPADRGLPWAGHLGLSLLPQVFERLFKARTSLLFTNTRAQAELW
                     HRALESIWPEDPATLALHHGSLAPRLRAAAEQGLHDGSVRCVVATSSLDLGVDFPAVD
                     QVLQIGSPKGVARLLQRAGRARHRPGASGRVVCVPAQALELIEYAAARQAIARGEIDS
                     RPPLRGCLDVLAQHAVTLALGGGFEPAALYGEVRETHAYAALDPVTWQAVLDFIEQGG
                     KALANYPEFRRVQRDDDGWCRVHDRRVALHHRLSIGTITADGAVSVKYLRGGGLGSVE
                     EGFLARLRPGDRFQFAGRTLQLVRLEGMTAYVRRAKDGDGPVASWQGGRMPLSTQLGR
                     EVESLCAHPGAHPEMRAAAPLLRIQQQASALPDRGRLVVERIGTRRGMHVFLFPFSGR
                     AVHEGMAAMMALRWGRLQPNTFSYTVNDYGLMLTLAEAAPLDEALLRRLLCPQGMADD
                     LRDGVNLGELARRQFREIARVAGLLTPALPGREARSLRQVQASSGLLYDVLRRYDPGH
                     LLLAQAEREVFERELEAPQLMAALRDCEARTLVLGEPPALTPLSFPLWTASLRGQLST
                     ETWQARVKRAAEQLEKRYARGP"
     gene            complement(8117..>8431)
                     /locus_tag="VGC69_16505"
     CDS             complement(8117..>8431)
                     /locus_tag="VGC69_16505"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391180.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0296932.1"
                     /translation="LYTDYTFGLWEGDALVPVAKAYSGLDDKEILALDRWLRAHTRER
                     FGPVRSVEPVQVFELGFEGVNLSKRHKSGVAVRFPRILRWRHDKPAAEADRLDALKAL
                     AR"
BASE COUNT         1487 a         2923 c         2703 g         1318 t
ORIGIN      
        1 gagcagttcc ggctcgatat ctggcgcaga cagcaggagc tggcgacatg atcggaattt
       61 gcattcccgc gcacaacgaa gaagcgtaca tcggcaaatg cttgagatcg gtcatgcatg
      121 cggcgcgaca tccgctcctc aatggcgaac cggtggagat tgtggtcgtc ctggatacct
      181 gctccgacga aacggaaatg cacgcacgcc gttggcccgt gacatgcctg cataccgcct
      241 tgcgaaatgt cggcttcgcg cgcgcggccg gcgcccgcca tttgctcgcg gccggcgcgc
      301 ggtggctggc gttcacggat gccgacacgg tcgtgtcgca aagctggctt gtcgaccagc
      361 tatcgctaga ctctgatgtg gtctgcggca ccatcgacgt ctcggactgg gacgagcacg
      421 gtgtatacga gacgctgacg cgggcgttct tcctctctta ctacaaagac gaggacggcc
      481 atcggcacgt ccatggcgcc aatcttggta ttgacgcgat ggcttatcag tcggtgggcg
      541 gattcgaatc gctggcttgc agcgaggatc gcgaaatcgt cgaacgactc gagcgcgccg
      601 gcgcctccat cgcctggacc gccctgcccc gcgtgacgac aagtgcacgg ccgttttccc
      661 gtgtcgcggg aggatttgcg accgccttgc gacaagcctg gcctgaagac ggccaggcgc
      721 ttttcccaga tgttcccccg agcccgacct gaaggagtag cccatggcaa cacgcaagcg
      781 tcagccgacc aaaatgccgc aacaacccgc attggatacg gcgtatttca actcggacag
      841 cgcgcccgcc ctgccgcccg cgcccgcggc ctcccagccg gcattgaaac aggcgcgggc
      901 ggtgggagaa acggtagacg gtgtgcccgc caattccaac aaggcggccg aatatggtga
      961 gaaggcggcc cggccgccag tcggcgccac cgtcgaaccg ccagacgaat ccgtcacggc
     1021 cagcacgctg tcggaaaaaa accggtccgc caagaccggc gccaaggcga gcgtcggcgt
     1081 gaatgcggcg agtggcgaat tgccgcgcgt gcgcgccgac agcgggggcc aggccatgac
     1141 caccaaccag ggcgttcccg tcgcggacaa ccaaagttcg ctcaaggcgg gcctgcgcgg
     1201 cccggctctg ctcaaggact tcatcctccg cgagaagatc acccatttcg accacgaacg
     1261 catcccggaa cgcattgtgc acgcccgggg ttcggccgcg cacggctact tcgagtgcta
     1321 cgaaccgctg acggacctga cctgcgcatc gctgttttcc gaggcgggca agcgcacgcc
     1381 ggtcttcgtg cggttctcta cggtggccgg cgagcgcggc tccaaggaca ccgcccgcga
     1441 cgtgcgcggc ttcgcggtga agttctatac cgatcagggc aactgggacc tggtcggcaa
     1501 caacattccg gtgttcttca tccaggacgc catgaagttt ccggatctcg tgcacgcggt
     1561 caagccggag cctcatcacg gcatgccgca ggcagcctcc gcgcacgaca cgttctggga
     1621 cttcatctct ctgatgcccg agtcgatcca caccatcatg tgggtcatgt cggatcgcgg
     1681 catcccccgc agctaccgca tgatgcaggg attcggcgtc cataccttcc gtttcgtcaa
     1741 cgccgcgggc gccgcgaagc tggtcaaatt ccactggaac cccaagctcg gcacgcattc
     1801 ccatgtctgg gacgaagcgg tcaagatctc cggcgccgat ccggatttcc atcgccgcga
     1861 cctgtgggaa gccatcgagg ccggcgcata cccggaatgg gaactcggcg tgcaaattgt
     1921 cgacgaagcg caagccgaca agttcagctt cgacgtgctg gacgccacca agctcatccc
     1981 cgaggagctg gtgcccgtca aacccatcgg ccgcctggtg ctcaaccgca acccggacaa
     2041 cttcttcgcc gagaccgaac aggtggcctt ctgcaccgcg catgtcgtgc cgggcgtcga
     2101 cttcaccaac gacccgctgc tggccgggcg catccactcc tatgtggaca ctcaaatctc
     2161 gcgcctgggc ggaccgaatt tccacgaact gccgatcaat gcgccgctgg cgcccgtaca
     2221 caacaaccaa cgcgacggca tgcaccgcca ggcgattcat cgcgggcgcg tggcctacga
     2281 gccgaactcc ctggcaggcg ggtgcccgtt ccaggccggc acgatgggat tcctgccctt
     2341 tcccgagccg gttgccgcgg acgaactgcg cggcaagccc gagaaattcg ccgagcacta
     2401 taaccaggcg gcgctcttct acgagagcca ggccggccac gagcagcagc acatcatcga
     2461 ggccttttcg ttcgaattga gcaaggtgac cgttcccggc atccgccggc gtaccgtggc
     2521 catgttgcgc aatgtgtcgg aagacctggc caatgccgtg gcagccaacc tgggcatgcc
     2581 cgaactgccg cagccgctgc ctcgggccgc caagacgaat accaaaccgg aaatcgcgac
     2641 atcggaattt ctgtccctgc tggcccgtcc gggcgacggc ggcatccggt cccgcaaggt
     2701 ggccatcatg gcggccgacg gcgtggacgc ccaagccgcg caacaagtgg cgcaagccct
     2761 gatcgccaag ggagcggtcg tccgcctggt cggccagcat gtgggactga tggcgggagc
     2821 cggcggggaa acgatggatg cggacgcctc gttcaagaac agcccgtcgc ccttgttcga
     2881 tgccgtggtc gtgcccgatg gcgcacaagc catcgatgcc ctgtgcgcgg acggcgtggc
     2941 gctggaattt gtgcgcgatg cgttccggca cggcaagacg ctcatggcga tcggcgaagg
     3001 gccaagactg ctgcagcatg ccggcatccc cgtcgacgac gcggaccccg gcctggtcct
     3061 ggctgcggct gcggagaaat ccgccatcgc ggcattcatc aatgcgttgg gaaaacaccg
     3121 tcatccggcc cgcgaatccg atccgcccgt ggtgtgacga tgcgatgcgc cgggatcggt
     3181 cggcgcgcgt cgaactatca gtctctctga caacccgagg aacacaacga tgtctgccaa
     3241 caatatctac gaagccttgc gggaaagcca tgcagtccaa cgttcgctct gcaggaagct
     3301 gctgcgcgcg gcgccccaca gcgaacgccg ccgggaaatc taccgggagc tcaaaatcga
     3361 gcttgccgcg catgccgcag cagaagaacg gtttctttat gccccgatcc tgatggacga
     3421 catggggctg gattcctcgc gccatgccct gtccgaacat catgagatcg acgagtatgt
     3481 ggaagaactg ggcaaggccg acgcacaagg cggaagctgg ctggccaagg cgcgcaagct
     3541 gtcggacgaa gtccatcacc acttgcgcga agaagaaaag aaattctttc aggtctccgg
     3601 caagatattg accgaaacac agaaggaaaa actggctcgg caatatttga aggactatca
     3661 gcggatgaag acgcgattgg cgcagagctg aaccgttcca cacagtcgac gcgggcaagc
     3721 gccaacggcc ggcagatcgg ccgttggcct gccccttcca ttcttaaaga tgccgggagt
     3781 tcccatgtcg cgcaagcagg actgcaagga tgaatacaag acggcggacg agctctcgca
     3841 tacccttcca tcgaaggcag tggccgttca tgagatcatc cgcaaagaag gcgaaaaaga
     3901 gctgagccgg gattttctcg cgcttttctg gtcggccatt gccgcgggac tgaccatgag
     3961 cacatccatg gtggggcgcg gggcaatgca agcctatttg ccggacgagc gctgggcttt
     4021 tctcatcgag gcggcaggct atacgctggg tttcattttc gtcatcacgg ccggccagca
     4081 actctttaca gagaataccg tcacgccagt cctgcctttc atgtcccggc cgagcatccg
     4141 ccagctggcc cgcctgctac ggctatgggg catcgtgttg ctgggaaacc tcatcggcgg
     4201 gctgatcgcc gccgccgtgt ttgcctggct gccgatgttt ccgccggaaa tcgtcgaagc
     4261 cttccgcaag atcgggcgcc accttctgga gacgccgatt ctcaccaatt ttgaaaccgc
     4321 catcctcgcc ggatggctca tcgccaccct ggtctggatg ctgcgcgccg tggagaacgg
     4381 aaaaatcgcc ctcatttttc tcgttaccta tctgatgggc atcgccggtg tcgcccatgt
     4441 ggtggtcggc accatcgaag taggctatct cgtattgctt ggcgaggcgt ccgtcgccga
     4501 tgccttgtcg cgctccatca tccctaccct ggtcggcaat gtcatcggcg gcacattcat
     4561 ctttgcgctg atcagccatg cgcaggtgcg cgccgatatc tgaacagttc gccccggctt
     4621 tgcgcgttcg cggcggaact cttcgctacc ccctgatctt gccccaaaga taggcaatcg
     4681 cccccacgac ggccaccgcc gtgaacgggt gctggcggat catatccttc gcgcacacca
     4741 tggcgttctc caaggcgtcg cgcccatcgt ccagcgcttc atcgaccgcg ccctttgccg
     4801 atctcacgcg ccgtttcgcc tcgctggccg cgctgtcgat cacccctgtg gcgatatcct
     4861 cacctttctc ggccaagtcc ttggccttct cgactgcttt ttccgtactc atggagaatc
     4921 tcctgagata aatgaggaca gcctgatctt acctggcaaa cccgcttgaa ttgaaaccta
     4981 tggtttcagg accattccgt gcgcaatgtc acgccctgga tgccgggata gcgatcacag
     5041 tcccctcgac acatgccgcc aacgtatcgc caaggccggc ctcgacgagg cggccgccgg
     5101 taaacgcgga gaacgcggga agtaccgtca tggccggtct tagccaaaag acgggccagg
     5161 cgcgcggcac gcccggcagg cgggtcttcg gatgcacgtg acctgcgatc acatggcggt
     5221 cctgcgcgtc ggcttgcggc aggtggcgaa acacaaaggg cccatcggcc tgcacctcgc
     5281 ccagctgacg caggcccaga gcggcgccat ccagcgcgcg atcatggttg ccggcgagcg
     5341 cggcgacatc cagccgcgcg tgcgcccggc gccactgtgt ccaggccgcc cgccaactgg
     5401 cttgcgaggc cgggccgtgc agcacatcac cgacgatcca cagttcgtgc gccgccgtcg
     5461 ccgcgaccag gccggccaac cgcgccaggt cgtcctgcgt ggcgccgctg ggcacggcga
     5521 tgccggcccg gcggaatacg tggcccttgc ccaaatgcag gtcggcaatc accagccggc
     5581 ggcgcgcggg ccaatacagg gcgcgctcgc ccagcaggac cacgggctcg ccggccagtc
     5641 gcacggccag cgagccttca tgggccccgg gcatagcgct tctccagttg ttcggcggcg
     5701 cgcttgacgc gggcctgcca ggtctcggtg ctgagctgcc cgcgcaggct tgccgtccag
     5761 agcggaaacg acaggggcgt cagcgcgggc ggttcgccca acaccagggt gcgcgcctcg
     5821 cagtcgcgca gcgccgccat cagctgcggc gcctccaact cgcgctcgaa gacttcacgc
     5881 tcggcctgcg ccagcagcag atggccggga tcgtagcgcc gcagcacgtc gtacagcagg
     5941 ccgctggagg cctgcacctg gcgcagcgaa cgcgcttccc ggccgggcag cgcaggcgtc
     6001 agcaggccgg ccacccgcgc gatctcgcgg aactgccggc gcgccagttc gcccaggttg
     6061 acgccatcgc gcaggtcgtc ggccatacct tgcgggcaga gcagtcggcg cagcagcgcc
     6121 tcgtccagcg gcgccgcctc ggccagggtc agcatcaagc cgtagtcgtt cacggtgtac
     6181 gagaaggtgt tgggctgcag ccgtccccag cgcagcgcca tcatcgccgc catgccctcg
     6241 tgcaccgcgc gccccgagaa gggaaacagg aacacatgca tgccgcgccg cgtgccgata
     6301 cgctcgacca ccaggcgccc gcggtcgggc agcgcgctgg cctgctgctg gatgcgcaac
     6361 agcggcgcgg ccgcccgcat ctcggggtgc gcgcccggat gcgcgcagag gctttccact
     6421 tcgcgcccca gttgcgtgga cagcggcatg cggccgccct gccagcttgc gaccggaccg
     6481 tcgccgtcct tggcgcgccg cacataggcc gtcatgccct ccagccgcac cagctgcagc
     6541 gtgcggccgg cgaactggaa ccggtcgccc ggccgcagcc gcgccagaaa gccctcctcc
     6601 acagaaccca ggccgccgcc gcgcaggtac tttaccgaca ccgcgccgtc ggccgtgatg
     6661 gtgccgatgg acaggcggtg atgcagggcg acgcggcggt cgtgcacacg gcaccagccg
     6721 tcgtcgtcgc gctgcacgcg gcgaaactcg ggatagttgg ccagcgcctt gccgccctgt
     6781 tcgatgaaat ccagcacggc ctgccaagtg acgggatcca gggcggcgta ggcgtgggtt
     6841 tcgcgcacct ccccatagag cgcagcgggt tcgaagccgc cgcccagcgc cagcgtcacg
     6901 gcgtgctgcg ccagcacgtc caggcagccg cgcaacggcg gacgcgaatc gatttccccg
     6961 cgcgcgatgg cctgccgggc ggccgcgtac tcgatcagct cgagcgcctg ggccggcacg
     7021 cagacgacgc gccccgacgc gccggggcga tgcctggcac ggccggcgcg ctgcagcagc
     7081 cgcgccacgc ccttggggct gccgatctgc agcacctggt ccaccgccgg gaaatccacg
     7141 cccaggtcca ggctcgaggt cgccaccacg cagcgcacgc tgccgtcgtg caggccctgc
     7201 tccgccgcgg cccgcagccg cggggccagc gagccgtgat gcagcgccag cgtggcggga
     7261 tcttccggcc agatcgattc cagcgcgcga tgccacagct ccgcctgggc gcgtgtattg
     7321 gtgaacagca gactggtgcg ggccttgaac aggcgctcga atacctgcgg cagcagggac
     7381 aggcccaggt gccccgccca gggcaggcca cgatcggccg gcagcagcag ggtcgagagg
     7441 gtgaccttgc gggccggcgc gccggatacc agcgggctgt ccggcgagtg cggcagcagc
     7501 acggcgcgcg cctcctgcag attgcccagc gtggccgaca ggccccaggt gcgcagcccc
     7561 ggcgacagcc gccgcaggcg ggccaggcac agttgcaaca gcacgccccg cttgttgccc
     7621 agtagctcgt gccactcgtc gaccacgacg cagcgcaggg cccgaaagcg cgaagacgca
     7681 tcggcatagg acagcagcag cgccagcgat tcgggcgtga tgaccaggac gtcggccttg
     7741 ccctggcggg ccaggcgctt gtcgcgcgcg ctggcatcgc ccgtgcgcag cgccacggtc
     7801 cagcccaggc ccagatcctg cgccacctgc gccagcgcct gggcggtgtc ggcggccaat
     7861 gcgcgcagcg gcgtgaccca cagcacgcgc ggtccgtcgc ccgccgccgt gcgcgcggcg
     7921 gcggggtcgg cgagcgcctg cagcaccggc ccgccgaagg cggccaaggt cttgccgctg
     7981 ccggtcgggg tgtgcagcaa gccggattcg ccgtccaggt agcgccgcca gacctcgcgc
     8041 tggaagcgcg cggccttcca gccgcgcgcg acgaaccagg cggccagcgg cccatcccag
     8101 ccgcgcggcc ccgctttcat cgcgccagcg ccttgagcgc gtcgaggcgg tccgcctcgg
     8161 cggccggctt gtcgtgccgc cagcgcagga tgcgcggaaa gcgcaccgcc acgcccgact
     8221 tgtgccgttt cgagaggttg acgccttcga agcccagctc gaacacctgg accggctcga
     8281 ccgagcgcac gggcccgaag cgctcacgcg tgtgggcgcg cagccagcgg tccagcgcca
     8341 ggatctcctt gtcgtccagg cctgaatagg ctttggccac gggcaccaac gcatcgcctt
     8401 cccacaggcc gaaggtgtag tcggtgtaga g
//
LOCUS       DASWPO010000317         4983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2131812, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000317 DASWPO010000000
VERSION     DASWPO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131812"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VGC69_16510"
     CDS             <1..102
                     /locus_tag="VGC69_16510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0296933.1"
                     /translation="TLARMMREAGLDRVQYFNLTAGVAALHEGVRLG"
     gene            209..1237
                     /locus_tag="VGC69_16515"
     CDS             209..1237
                     /locus_tag="VGC69_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEY0296934.1"
                     /translation="MHRRFPRLPLRGLLAGCLAVALAATALAPSAADARRLGGGSSFG
                     RQSTHITPRTATPSPAAPSATPYSGAAAPAAAPSAAAPASAAKPAAASAPAPAPAPAT
                     PARSGASRWLGPIAGIAAGLGLAALMSHLGLSGAFAEFLSSALLIAVAVFAVLFIVRR
                     LFGSAPRPALQGAGGPLLRESGPPPAMAPAAMPAAPQAGLALAAPPAEAEPVNWFIPG
                     DFDTAHFLQQAKAQFVNIQALWDTGEVDRLREYLTDDLIAELKPQLGARGGLSDKTEV
                     VLLNAEMLGIEAVSDGHLASVRFSGMLRESLGAEAFRFEEVWNLFKPTQGGWLLAGIQ
                     QIPVEQAS"
     gene            complement(1337..2653)
                     /locus_tag="VGC69_16520"
     CDS             complement(1337..2653)
                     /locus_tag="VGC69_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296935.1"
                     /translation="MSTLPSSSTLSPGSPSASTPEVDATYRKITVRLVPFLVFLFILA
                     WIDRVNIGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARATLAR
                     ITILWGLTSMAMIFVHTATTFYILRFLLGAFEAGFFPGVVLYLTYWFPSARRARINGL
                     FMTSFAIAGIVGGPVAGFVMSVASGLEGLTNWQWLFLIEGIPSVIAGFAVLAYLPKKP
                     DDAKWLSAGEKAMVKAEVVRDAADPAKKSSLRHAFTARVWLMSAIYFCVVSGNATIAF
                     WTPSIIKALGVQGNFTIGLVSAIPFIAGTIAMVWYGAHSDRSGERRMHCIIASIVATL
                     GLIGTGYFLDQPNLALLSLTIAAIGILAAFPVFWSLPAAFLAGTAAAGGIAVINSIGN
                     LAGFVAPYIMGWLKDSTGSLAAGLYFVGALELGAAVLILAFIRIKR"
     gene            complement(2740..4071)
                     /locus_tag="VGC69_16525"
     CDS             complement(2740..4071)
                     /locus_tag="VGC69_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0296936.1"
                     /translation="MSAPMKTVHELAAELAAGKTTSVKLVEDALARIDSYRAQGGAAY
                     LEVDAQSALQAARASDQARQAGYAPSVLAGLPVSIKDLFDVKGQVSKAGSIALDGAAP
                     AAQDAIAVARLRQAGAILLGRTNMSEFAFSGLGLNPHYGTPRAPHDAARVAGGSSSGG
                     AVSVALGMAAAALGTDTGGSIRIPSAFCGLTGFKPTAKRVPLAGAVPLSGSLDSIGPL
                     SVSVACCAAFDAAISGETLDARPAGIRGLRLYVTRDFVCDGLDADVERAFEAALQTLS
                     QAGAHIVPFDFPELHEIPRINAGGGLTAAESWAWHRKLLETDGPRYDQRVAQRIRRGS
                     ALSAADYLDILAARARIRAIAADRLRDADAWLMPTVAVKPPLLAPLETDDNAFFAANG
                     MVLRNTSVINFLDGCGVSLPTGEPGIGLSVCGLHGQDARVLQISAAAQALG"
     gene            complement(4068..4745)
                     /locus_tag="VGC69_16530"
     CDS             complement(4068..4745)
                     /locus_tag="VGC69_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEY0296937.1"
                     /translation="MASLNFKLAGAGDIAFSIDRLVVAGWAGRNAAAVQHHIDELAAL
                     GVKPPRHVPCYYPVAAALLTTGAEIEVPRADSSGEVEVVLLSSPQGLLVGIGSDHTDR
                     KAEAYDVTVSKQMCAKPVGAGLWRFDEVAGHWDELISRSWRTRNGVRELYQEGPMTSL
                     RDPRELMRAYAQADTLPEGTAMYCGTQPVLGELGYGEAFELELHDPRLNRSLRHAYTV
                     RALPVDE"
     gene            complement(4782..>4983)
                     /locus_tag="VGC69_16535"
     CDS             complement(4782..>4983)
                     /locus_tag="VGC69_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY0296938.1"
                     /translation="ADLLRGLHEKQARFWFMTLSDPAHMENVYDDHLALIDALERRDV
                     PQVREIVRHHVDEFRKTIIRSV"
BASE COUNT          744 a         1648 c         1865 g          726 t
ORIGIN      
        1 accctggccc gcatgatgcg cgaggccggc ctggaccgcg tgcagtactt caacctgacc
       61 gccggcgtgg cggcgctgca cgagggcgtg cggctgggtt gacgaccggc ccgccggcgc
      121 cggcggggat cgtcgcttgt ggcggacggg atagtccgtt atgcttgggt cattcagttt
      181 tgtgtaagac gccatgcgac ccgccctcat gcaccgccgt ttcccccgcc ttccgctacg
      241 cggcctgtta gccggctgcc ttgccgtggc cttggccgcg acggcgctgg cgccatcggc
      301 ggccgacgcc aggcgcctcg gcggcggttc gagctttggc cgccagtcga cccacatcac
      361 gccgcgcacc gcgacgccat cgcccgccgc gccatcggcc acgccgtatt cgggcgcggc
      421 cgcaccggct gccgccccgt ccgcggcggc gccggcttcg gcggccaagc ccgcggctgc
      481 ctcggcgccc gcccccgcgc ccgcgccggc caccccggcc cgcagcggcg cctcgcgttg
      541 gctggggccc atcgccggca tcgccgccgg cctgggcctg gcggccctga tgtcgcacct
      601 gggcctgtcg ggcgcgtttg ccgaattcct gtccagcgcc ctgttgatcg ccgtggccgt
      661 gtttgccgtg ctgttcatcg tgcgccgctt gttcgggagc gcgccgcgcc ccgccttgca
      721 gggcgcgggc gggccgctgc tgcgcgagtc cggtccgccg cccgccatgg cgcctgcggc
      781 catgcctgcg gcgccgcaag ccggccttgc cctggccgcg ccgcccgccg aggccgagcc
      841 ggtcaactgg ttcatcccgg gcgatttcga caccgcgcat ttcctgcagc aggccaaggc
      901 gcagttcgtc aatatccagg ccctgtggga taccggcgag gtcgaccgcc tgcgcgagta
      961 cctgaccgac gatctcatcg ccgagctcaa gccccagctc ggcgcgcgcg gcggcctgtc
     1021 cgacaagacc gaggtggtgc tgctcaacgc cgagatgctg ggcatcgaag cggtgtccga
     1081 cggccacctg gccagcgtgc gcttctcggg catgctgcgc gagtcgctgg gcgccgaggc
     1141 cttccgcttc gaagaagtgt ggaacctctt caagcccacg cagggcggct ggctgctggc
     1201 cggcatccag cagatcccgg tggagcaggc aagctgatct tgcctcgccc tgcacgggcc
     1261 ggttgcatgc cggcccatga aaaaaacggc gcccggcata atccgggcgc cgttttttca
     1321 gcgtgcctgg ccgcgatcag cgcttgatgc ggatgaaagc caggatcagc acggccgcgc
     1381 cgagttcgag cgcgccgacg aaatacagcc cggccgccag gctgcccgtc gagtccttga
     1441 gccagcccat gatgtagggg gcgacgaagc cggccaggtt gccgatcgag ttgatgacgg
     1501 cgatgccgcc cgcggccgcc gtgccggcca ggaaggcggc gggcagggac cagaacacgg
     1561 ggaaggcggc caggatgccg atggcggcga tggtgagcga cagcagggcc aggttgggct
     1621 ggtccaggaa gtagcccgtg ccgatcaggc cgagcgtggc gacgatggag gcgatgatgc
     1681 agtgcatccg gcgctcgccg ctgcggtccg agtgcgcccc gtaccacacc atggcgatgg
     1741 tgcccgcgat gaacggaatg gccgagacca ggccgatcgt gaagttgccc tgcacgccca
     1801 gcgccttgat gatggacggt gtccagaagg cgatggtggc gttgccgctg accacgcaga
     1861 aatagatggc cgacatcagc cagacgcggg ccgtgaaggc atggcgcagc gaagacttct
     1921 tggccgggtc ggccgcgtcg cgcacgactt ccgccttgac catggccttc tcgccggcgc
     1981 tgagccactt ggcgtcgtcg ggcttcttgg gcaggtaggc cagcaccgcg aaaccggcga
     2041 tgaccgaggg aatgccctcg atgaggaaca gccactgcca gttggtcagg ccttccaggc
     2101 ccgaggccac gctcatcacg aaaccggcga ccgggccgcc gacgatgccc gcgatggcga
     2161 acgaggtcat gaacaggccg ttgatgcgcg cgcggcgggc cgacgggaac cagtaggtca
     2221 ggtacagcac cacgcccggg aagaaaccgg cctcgaaggc gcccagcagg aagcgcagga
     2281 tgtagaacgt ggtggccgtg tgcacgaaga tcatcgccat cgaggtcagg ccccacagga
     2341 tggtgatgcg cgccagcgtg gcccgcgcgc cgatcttctc gagcagcagg ttgctaggca
     2401 cttcgaacag gaagtagccg atgaagaaga tgccggcgcc caggccgtag acggcttcgc
     2461 tgaactgcag gtcctgcagc atctgcagtt tggcaaagcc gatgttgacg cggtctatcc
     2521 aggccaggat gaacaggaag accaggaagg gaacgagtcg taccgtgatc ttccggtagg
     2581 tggcgtcgac ctcgggcgtg gatgccgatg gggagccggg gctcaaggtg ctgctagacg
     2641 ggagggtaga cataggagtt atcgaaccag gaggcgagtc caggaaggcg ctgccgaatc
     2701 ggcccgcggc agcgacgggc aatgcgcggt gcgcggcgct cagccgagcg cttgcgcggc
     2761 agcggaaatt tgcagcacgc gggcgtcctg gccatgcagg ccgcagacgg acaggccgat
     2821 gcccggctcg cccgtgggca gcgacacgcc gcagccgtcc aggaagttga tgacgctggt
     2881 gttgcgcagc accatgccgt tggcggcgaa gaaggcgttg tcgtccgttt ccagcggcgc
     2941 cagcaggggc ggcttgacgg cgacggtcgg catcagccag gcatcggcgt cgcgcagccg
     3001 gtcggccgca atggcccgga tgcgcgcgcg cgcggcgagg atgtccaggt agtcggccgc
     3061 gctcagggcg ctgccgcggc ggatgcgctg cgcgacgcgc tggtcgtagc gcgggccgtc
     3121 ggtctccagc agcttgcggt gccaggccca cgactcggcc gccgtcaggc cgccgccggc
     3181 gttgatcctg gggatctcgt gcagctcggg gaagtcgaag ggcacgatgt gggcgccggc
     3241 ttgcgacagc gtctgcagcg cggcctcgaa ggcgcgttcc acgtcggcat ccaggccgtc
     3301 gcagacgaag tcgcgcgtca cgtacaggcg caggccgcgg atgccggcgg ggcgcgcgtc
     3361 gagcgtctcg ccgctgatcg cggcgtcgaa ggccgcgcag caggccaccg acacggacag
     3421 ggggccgatg gaatcgagcg agcccgacag cggcacggcg cccgcgagcg gcacgcgttt
     3481 ggcggtgggc ttgaagccgg tcaggccgca gaaggccgag gggatgcgga tggagccgcc
     3541 ggtgtcggtg cccagcgcgg cggcggccat gcccagcgcc acgctgaccg cgccgcccga
     3601 gctcgagccg ccggccacgc gggcggcgtc gtgcggcgcg cgcggcgtgc cgtagtgcgg
     3661 gttcaggccc aggcccgaga aggcgaattc gctcatgttg gtgcggccca gcaggatggc
     3721 gccggcctgg cgcagccgcg ccaccgcgat ggcgtcctgc gccgcgggcg cggcgccgtc
     3781 gagcgcgatg gagccggcct tgctgacctg gcccttcacg tcgaacaagt ccttgatgga
     3841 caccggcagg ccggcgagca cgctgggcgc gtagccggcc tggcgcgcct ggtcgctggc
     3901 gcgggcggcc tgcagggccg actgggcgtc gacctccagg taggccgcgc cgccctgggc
     3961 gcggtagctg tcgatgcggg cgagggcgtc ctcgaccagt ttgacgctgg tggtcttgcc
     4021 cgcggcgagt tccgcggcga gttcatgcac agttttcatg ggggcgctca ttcgtcgacg
     4081 ggcagcgcgc gcacggtgta ggcatggcgc aggctgcggt taaggcgggg atcgtgcagt
     4141 tcgagctcga aggcttcgcc gtagccgagc tcgcccagga cgggctgggt gccgcagtac
     4201 atggccgtgc cttcgggcaa ggtgtcggcc tgggcatagg cgcgcatcag ctcgcgcggg
     4261 tcgcgcaggc tggtcatggg gccttcctgg taaagctcgc gcacgccgtt gcgggtgcgc
     4321 caactgcggg agatcagttc gtcccagtgg ccggccacct cgtcgaagcg ccagagcccg
     4381 gcgccgacgg gcttggcgca catctgcttg gacaccgtga cgtcataggc ctcggccttg
     4441 cggtcggtat ggtccgagcc gatgcccacc agcaggccct ggggcgagga cagcagcacg
     4501 acctcgacct cgccggagga gtcggcgcgc gggacttcga tctcggcgcc ggtggtcagc
     4561 agcgcggcgg ccaccgggta gtagcagggc acatgcctgg gcggcttgac gcccagcgcg
     4621 gcgagctcgt cgatgtggtg ctgcacggcg gcggcgttgc ggccggccca gccggcgacg
     4681 accaggcggt cgatggaaaa ggcgatgtcg ccggcgccgg cgagtttgaa attcaatgaa
     4741 gccatgatgt gcaacctagg ggtatcgggg gaggaaaaag gtcagacgga tcggatgatg
     4801 gtcttgcgga attcgtcgac gtggtggcgc acgatctcgc gcacctgggg cacgtcgcgg
     4861 cgctccagcg catcgatgag ggcgaggtgg tcgtcgtaga cgttttccat gtgggcgggg
     4921 tcggacagcg tcatgaacca gaagcgggcc tgcttttcgt gcaggccgcg cagcaggtcg
     4981 gcc
//
LOCUS       DASWPO010000318        10369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1235041, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000318 DASWPO010000000
VERSION     DASWPO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235041"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGC69_16540"
     CDS             <1..273
                     /locus_tag="VGC69_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY0296939.1"
                     /translation="RVAELLILRGDMPRSLLASMRAVAETLAMISNGRSSETERRAGM
                     MRSELEYSRLEDIMRGGLHRFLERFLARINDLGNRISQDFLVPLST"
     gene            342..1151
                     /locus_tag="VGC69_16545"
     CDS             342..1151
                     /locus_tag="VGC69_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0296940.1"
                     /translation="MKHLIKHVTRYRYSAPVNYTIQTLRLTPRADDHQRALRWQISAP
                     GPLTEQVDAYGNVTHTLTLNHPHNEIEIRVTGQIEIDPLDQGMIHGEDSRLPLHAYCV
                     PTPLTQCDENIRQLCREAVPNGIHTPQDLLALAGAICDRVTYEPGYTDVTTAADQVLA
                     LGHGVCQDHAHLFLACVRGLGLPARYVSGYHYANAADDVASSHAWADVWLPNQGWVSV
                     DITNRALTSDNHCRLAVARDYDSASPVRGVRNGGGEESMTISVQVQRAKQQ"
     gene            1161..4514
                     /locus_tag="VGC69_16550"
     CDS             1161..4514
                     /locus_tag="VGC69_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0296941.1"
                     /translation="MAIHVALKHVTHYTYDRLVSLSPQVVRLRPAPHCRTPILSYSLH
                     VEPAQHFVNWQQDPFANYLARLTFPEKTREFKITVDLVAEMAVYNPFDFFLEASAERY
                     PFKYEAGLAHELAPYLAHQPVTPLLADFLERVDKRSQRTVDFLVGINQLLHQEIRYLI
                     RMEPGVQAPEETLRLRSGSCRDSGWLLVQALRHLGLAARFVSGYLIQLKPDVKALDGP
                     SGTEVDFTDLHAWCEVFLPGAGWIGLDPTSGLLAGEGHIPLAATPEPGSAAPVTGAVD
                     KSEVEFSHVMEVTRIFESPRVTKPYSEDQWLAIKDLGHVIDDDLKAGDVRLTMGGEPT
                     FVATSDRDAAEWNTAALGPTKRGYATELVLRLRERYGANGFLHFGKGKWYPGEQLPRW
                     ALYIYWRADGQPCWQNPGLFADERHPGRYTEADAQRFMTELCAQLGLDAQYVKPGHED
                     VFYYLWRERRLPVNVDPFDSRLDDELERTRLRKIFEQKLDKVVGYALPIRRGEGPALD
                     GPAWITGPWFFRDERMYLFPGDSPMGYRLPLDSLPWAAETDRPLAIERDPFDTRTDLP
                     SPAQVRAQLRSGQAQAAGTQGAAADGPAGDGAGTTPYPQRFESAAWLTRTAMCTEARG
                     GNLYVFMPPLQKVEDYLELLAAVEATAAKLGVRIVIEGYPPPRDARLKVLQVTPDPGV
                     IEVNIHPAANWDELIDHTEFLYEAAFQTRLCTEKFMVDGRHTGTGGGNHFVLGGATPA
                     DSPFLRRPDLLSSLLGYWLNHPSLSYLFSGLFIGPTSQAPRIDEARNDQVFELEIAFQ
                     EIRKQLAAHRGQVPPWMVDRALRNILVDVTGNTHRAEFCIDKLYSPDSGTGRLGLLEL
                     RAFEMPPHARMSLAQQLLLRGLVAHFWKHPYTAPITRWGTVLHDRFLLPTFVQMDFED
                     VIADLNAAGYGFDAAWFAPHFEFRFPLYGDIAARGINLEIRGALEPWHVMGEEGSAGG
                     TVRYVDASLERIEIKISGLNDSRYVITVNGRALPLQPTGRAGEFVAGVRYKAWAPPSA
                     LHPTIGVHAPLTLDVVDTWSHRSLGGCQYFVDHPGGLNPDLLPVNAYEAESRRLARFR
                     KMGHTPGRMTATPAVPGREFPFTLDLRHD"
     gene            4555..7167
                     /locus_tag="VGC69_16555"
     CDS             4555..7167
                     /locus_tag="VGC69_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY0296942.1"
                     /translation="MPQLLFQTDPSRDATSLALHAALPARAGHYDELRPAADPAGGSA
                     TLRPAWARFFELLGPEGLSELDRNAGAVARQIQENGITYNIYADADGPTRPWSLDLLP
                     FIIDETDWRLIERGLSQRACLLNQVMADLYGPQTLLAEGLLPPALVQGHPGFLRPLRG
                     YTPPGGVYLHIAAFDLARDGEGSWHVVSQRTQAPSGLGYLLENRVTISGMFSEAFKEL
                     RVQHLAASYRRLIDMLHQLSPAGTDGAPPRIALLTPGPYNETYFEQTYLARYLGLTLV
                     EGGDLLVRDNQLFLKTLHGLERVHALLRRLDDDYCDPLELRSDSALGIPGLLQAMRAG
                     NVLVANALGTGLLESPALYGFLPAISQRLTGEALVLPSLPSWWCGEAAALEQALPMLD
                     RAVIKSTYPNSRQRKSFEPLIGAGADKARLAALRQRIEQSPDAHTVQAYLPLSQAPTW
                     HDGRIVPRAAMVRVFAMADGQGGWHVLPGGLTRIANREQQVVSMQRGGSSMDTWVTTD
                     GPVDLFSMLPTSMRPEDVAQARRPVSSRAAENLFWMGRYAERLGNDVRLANVTLTWLN
                     GDLDNPDTLSRALARLCTRSGLVPAELPRSTRVFEHTLVAELASRGTQRGFARNLSSL
                     TFAAGQIRDRLSPEHWQLIVSAGARYHRLIAGDLTDDASRSEPDEDAPPRTATDVLRA
                     LRRLNRQILSITGAQTDHMTRDDGWRLLTIGRHTERLSTMAGMLAALFEDNAILRDGG
                     FNLLLQLFDSAITYRAYYQGRYEIPALLNLLVLDPNNPRSPACSLDVLRATLTRLPGS
                     GAGPANDLLALLPTPDLGLTLSQLCHRDANGRYTEVLALARRLEAAAAALSDEISRRY
                     FSHSADVDQSLAAA"
     gene            7178..8131
                     /locus_tag="VGC69_16560"
     CDS             7178..8131
                     /locus_tag="VGC69_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY0296943.1"
                     /translation="MNTLEPRPLAVVHETHYHYDAPVELAHHLSYLRPRDDAHQTVES
                     HTLRISPTPNDVLQSMDDQGNWRSTFALSAPHDELVVHACSRVVVRPRYAGLDPAAGM
                     PWEAAAERLRYRAGAPYAPEVEYAFPSPFVPLHDALRDYALASFTPDRPLPEAAIDLM
                     HRIHADFTYAPASTQIATPVLEAFEEKRGVCQDYAHVMLGCLHTLGLAARYVSGYLVS
                     KPPPGQPRLIGADASHAWISVHCPGLAANGGWLDLDPTNDLIASTEHVTLAYGRDYGD
                     VTPLRGVIRGGGGHELTVHVTVMPWDEAPAGLMQGLAQFQD"
     gene            8294..9460
                     /locus_tag="VGC69_16565"
     CDS             8294..9460
                     /locus_tag="VGC69_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0296944.1"
                     /translation="MPIIEASDSNATVLGLFTTHRNLISLTVVEDARPIGLINRDIFL
                     SQMSKPFHREIYDRKSCIAFMDKEPLIVDAGTGIEALTLKTIEYGEKALTDGFIVTQE
                     DRFLGLGNGLQLMSAVAAIQAEKNRQTMQSIEYASVIQRAMLRASREALTATPHQAAL
                     VWEPRDVVGGDFYQFASHPDGWFGAVADCTGHGVPGAFMTLLASSALAKALEQLGPRD
                     PAALLSAVNRSVKTMLGQMGKAGESSESDDGMDAAFFWFDAQRRELRFSGARIAVHIL
                     RPGAQAFDVLAGQRMGVGYVDSDADYVWAQKTAPLPPGSLLFITTDGYTDQIGGPRKI
                     AYGKRRAHDVILACRDEPVGVVCENLTQALAQWQGTQPRRDDITLFCTRIEEHR"
     gene            9457..10023
                     /locus_tag="VGC69_16570"
     CDS             9457..10023
                     /locus_tag="VGC69_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HEY0296945.1"
                     /translation="MNIEHSMGQDRVFLQLARSRNVLFYHKGYFSQNIIAAMGEVVRL
                     QLEVAGVEGPTRRKLFSSFVELAQNIMHYSSDTIPPDSADSSARAGVVCIAAEDGHHV
                     MLCINPIATASVTALRDKLEPLRDMSLEQIKQAYKASLRANTPEDSKGAGLGFLTMAR
                     DASAPLDFAFQPRDDAPGTTQFYLKAII"
     gene            10050..>10369
                     /locus_tag="VGC69_16575"
     CDS             10050..>10369
                     /locus_tag="VGC69_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HEY0296946.1"
                     /translation="MENLYIAATATSPEVDFRFEQHQLSLTGESYPENAAAFYTPIIE
                     RLRAYLAGCDDTAVTVDVTLAYFNSSSTKMLFSIFDALDQAAQSGNRVVLNWHYDAED
                     ETIL"
BASE COUNT         1691 a         4027 c         3123 g         1528 t
ORIGIN      
        1 cgcgtggccg agctgctcat cctgcgcggc gacatgccgc gctcgctgct ggcctcgatg
       61 cgcgccgtcg ccgaaaccct ggccatgatc tccaacggcc gctcttccga gaccgagcgg
      121 cgcgccggca tgatgcgctc cgagctcgag tacagccgcc tcgaggacat catgcgcggc
      181 ggcctgcacc gcttccttga acgcttcctg gcccgcatca acgacctggg caaccgcatc
      241 agccaggact ttctggtgcc gctatcgaca tagatccgca acctatacgc cctacattgc
      301 gacagcgcca cccgacttcg cgcacttccg agggaattgc catgaaacac ctcatcaagc
      361 acgtcacccg ctaccgctat agcgcgccgg tcaactacac catccagacg ctgcggctga
      421 ccccgcgcgc cgacgaccac cagcgcgccc tgcgctggca gatctccgcc cccggcccgc
      481 tcaccgagca ggtcgacgcc tacggcaacg tcacgcacac gctcacgctc aaccatccgc
      541 acaacgagat cgagatccgc gtcaccggcc agatcgagat cgacccgctc gaccagggca
      601 tgatccacgg cgaagacagc cgcctgccgc tgcacgccta ttgcgtgccc acgccgctca
      661 cgcagtgcga cgagaacatc cgccagctct gccgcgaggc cgtgccgaac ggcatccata
      721 cgccgcaaga cctgctggcc ctggccggcg ccatctgcga ccgcgtgacc tacgagcccg
      781 gctacaccga cgtcaccacc gcggccgacc aggtgctggc gctgggccac ggcgtctgcc
      841 aggaccatgc ccacctgttc ctcgcctgcg tgcgcggcct gggcctgccg gcccgctacg
      901 tcagcggcta ccactacgcc aatgccgccg atgatgtcgc ctccagccac gcctgggccg
      961 acgtctggct gcccaaccag ggttgggtca gcgtggacat caccaatcgc gcgctcacct
     1021 ccgacaacca ttgccggctg gcggtggcgc gcgactacga ctcggcctcg ccggtgcgcg
     1081 gggtgcgcaa cggcggcggc gaagagtcga tgaccatttc cgtgcaagtg caacgcgcca
     1141 agcagcaata ggagagcccc gtggccatac acgtcgcgct caagcatgtc acgcattaca
     1201 cgtacgaccg gctggtcagc ctgtcgcccc aggtcgtccg gctgcggccc gcgccgcatt
     1261 gccgcacgcc catcctgtcg tactcgctgc acgtcgagcc ggcgcagcac ttcgtcaact
     1321 ggcagcagga cccgttcgcc aactacctgg cgcgcctgac ctttcccgag aagacgcgcg
     1381 agttcaagat cacggtcgac ctcgtggccg agatggccgt ctacaacccc ttcgacttct
     1441 tcctggaggc gtcggccgag cgctatccct tcaagtacga agcgggcctg gcgcacgaac
     1501 tggcgcccta cctggcgcac cagcccgtca cgccgctgct ggccgatttt ctcgagcgcg
     1561 tcgacaagcg ctcgcagcgc acggtggact tcctggtggg catcaaccag ctgctccacc
     1621 aggaaatccg ctacctgatc cgcatggagc cgggcgtgca agcgccggaa gaaaccctgc
     1681 gcctgcgctc gggctcctgc cgcgactcgg gctggctgct ggtgcaggcc ctgcgccacc
     1741 tgggcctggc cgcgcgcttc gtgtcgggct acctcatcca gctcaagccc gacgtcaagg
     1801 cgctcgacgg ccccagcggc accgaggtcg acttcaccga cctgcacgcc tggtgcgagg
     1861 tcttcctgcc cggcgcgggc tggataggcc tggaccccac ctcgggcctg ttggccggcg
     1921 aaggccatat cccgctggcc gccacgcccg agccgggcag cgccgcgccg gtcaccggcg
     1981 cggtcgacaa gagcgaggtc gagttctcgc acgtcatgga agtgacgcgc atcttcgaat
     2041 cgccgcgcgt caccaagccc tattccgagg accagtggct ggccatcaag gacctcggcc
     2101 acgtcatcga cgacgacctc aaggccggcg acgtgcgcct gaccatgggc ggcgagccca
     2161 ccttcgtggc cacctccgat cgcgacgcgg ccgaatggaa caccgccgcg ctcggcccca
     2221 ccaagcgcgg ctacgccacc gagctggtgc tgcgcctgcg cgagcgctat ggcgccaacg
     2281 gcttcctgca cttcggcaag ggcaagtggt acccgggcga gcagctgccg cgctgggccc
     2341 tgtacatcta ctggcgcgcc gacggccagc cctgctggca aaaccccgga ctcttcgccg
     2401 acgaacggca cccgggccgc tataccgagg ccgacgcgca gcgcttcatg acggagctct
     2461 gcgcccagct cgggctggac gcgcaatatg tgaagcccgg ccatgaagac gtgttctatt
     2521 acctgtggcg cgagcgccgc ctgccggtca acgtcgaccc cttcgactcc cgcctggacg
     2581 acgagctcga acgcacccgg ctgcgcaaga tcttcgaaca gaagctcgac aaggtagtgg
     2641 gctatgccct gcccatccgc cgcggcgaag gcccggccct ggacggcccg gcctggatca
     2701 ccggcccctg gttcttccgc gacgagcgca tgtacctgtt cccgggcgat tcgcccatgg
     2761 gctatcggct gccgctcgat tcgctgccct gggccgcgga aacggatcgc cccctcgcca
     2821 tcgaacgcga tcccttcgac acgcgcaccg acctgcccag cccggcgcag gtccgcgccc
     2881 agctgcgcag cggccaggcc caggccgccg gcacccaggg cgccgcggcc gacggcccgg
     2941 caggcgacgg cgcgggaacc accccctacc cgcagcgctt cgaatcggcc gcctggctca
     3001 cccgcacggc catgtgcacc gaggcgcgcg gcggcaacct ctacgtcttc atgccgccgc
     3061 tgcagaaggt ggaagactac ctcgagctgc tcgccgcggt agaagccacg gccgccaagc
     3121 tgggcgtgcg catcgtgatc gagggctacc cgcccccgcg cgacgcgcgc ctgaaggtgc
     3181 tgcaggtcac gcccgacccc ggcgtgatcg aggtcaacat ccacccggcc gccaactggg
     3241 acgaactcat cgaccacacc gagttcctct acgaggcggc cttccagacc cgcctgtgca
     3301 ccgagaagtt catggtcgac gggcgccaca ccggcaccgg cggcggcaac cacttcgtgc
     3361 tgggcggcgc cacgccggcc gacagcccct tcctgcgccg gcccgacctg ctctccagcc
     3421 tgctgggcta ctggctcaac cacccctcgc tgtcctacct gttctcgggc ctgttcatcg
     3481 gccccaccag ccaggcgccg cgcatcgacg aggcgcgcaa cgaccaggta ttcgagctcg
     3541 agatcgcctt ccaggaaata cgcaagcagc tcgccgccca ccgcggccag gtgccgccct
     3601 ggatggtgga ccgcgccctg cgcaacatcc tcgtcgacgt caccggcaac acgcaccgcg
     3661 ccgagttctg catcgacaag ctctattcgc cggacagcgg caccggccgc ctgggcctgc
     3721 tcgagctgcg cgccttcgag atgccgccgc acgcgcgcat gagcctggcg cagcaactgc
     3781 tgctgcgcgg cctggtggcg cacttctgga agcatcccta caccgcgccc atcacccgct
     3841 ggggcacggt gctgcacgac cgcttcctct tgcccacctt cgtgcagatg gacttcgagg
     3901 acgtcatcgc cgacctcaac gcggccggct acggcttcga cgccgcctgg ttcgcgccgc
     3961 acttcgagtt ccgcttcccg ctctacggcg acatcgccgc gcgcggcatc aacctggaga
     4021 tccgcggcgc gctcgaaccc tggcacgtga tgggcgagga aggctcggcc ggcggcacgg
     4081 tgcgctatgt cgacgcctcg ctggagcgca tcgagatcaa gatctcgggc ctgaacgaca
     4141 gccgctacgt catcaccgtc aacggccgcg ccctgcccct gcagcccacc ggccgcgccg
     4201 gcgagttcgt ggccggcgtg cgctacaagg cctgggcgcc gccctcggcg ctgcacccga
     4261 ccatcggcgt gcacgccccg ctcacgctcg acgtcgtcga cacctggtcg caccgctcgc
     4321 tgggcggctg ccagtacttc gtcgaccacc ccggaggact caatcccgac ctgctgccgg
     4381 tcaacgccta cgaggccgaa agccgccgcc tggcgcgatt ccgtaaaatg ggccatacac
     4441 cgggccgcat gaccgcgacc ccggccgtgc ctggccgcga attcccgttc acgctggatt
     4501 tgcggcacga ctgaccccac gccccggcat gccggggcgt gcctagccca ccccatgccg
     4561 cagctgctct ttcaaaccga cccctcgcgc gatgccacct cgctggccct gcacgcggcc
     4621 ttgcccgcgc gcgccggcca ttacgacgaa ctccggccgg ccgccgaccc ggcgggcggc
     4681 agcgcgaccc tgcggccggc ctgggcccgc ttcttcgaac tgctgggtcc cgaaggcctg
     4741 tccgaactcg accgcaatgc cggcgccgtg gcccgccaga tccaggaaaa cggcatcacc
     4801 tataacatct acgccgatgc cgacggcccc acgcgcccct ggtcgctgga cctgctgccc
     4861 ttcatcatcg acgagaccga ctggcgcctg atcgagcgcg gcctgtcgca gcgggcctgc
     4921 ctgctcaacc aggtcatggc cgacctctac ggcccgcaga cgctgctcgc cgaaggcctg
     4981 ctgccgccgg ccctggtgca gggccatccc ggctttctgc gtccgctgcg cggctacacg
     5041 ccgcccggcg gcgtgtacct gcacatcgcg gccttcgacc tggcgcgcga cggcgagggc
     5101 agctggcacg tggtctcgca acgcacccag gcgccttccg gcctgggcta cctgctcgag
     5161 aaccgcgtca ccatctcggg catgttctcc gaggcgttca aggaactgcg cgtgcagcac
     5221 ctggcggcca gctaccgccg gctgatcgac atgctgcacc agctcagccc ggccgggacg
     5281 gacggcgcgc cgccgcgcat cgcgctgctc acccccggcc cctacaacga aacctatttc
     5341 gaacagacct acctggcccg ctacctgggc ctgacgctgg tcgagggcgg cgacctgctg
     5401 gtgcgcgaca accagctctt tctcaagacc ctgcacggcc tggagcgcgt gcacgccctg
     5461 ctgcggcggc tcgacgacga ctactgcgac ccgctggaat tgcgctcgga ctccgcgctg
     5521 ggcatccccg ggctgctgca ggccatgcgc gccggcaatg tgctggtggc caacgcgctg
     5581 ggcacgggcc tgctggaatc gcccgccttg tacggcttcc tgcccgccat ctcgcaacgg
     5641 ctgaccggcg aggccctggt cctgccttcg ctgccgtcgt ggtggtgcgg cgaggccgcc
     5701 gccctcgaac aggccctgcc catgctggac cgcgcggtca tcaagtccac ctaccccaac
     5761 agccgccagc gcaagagctt cgagcccctg atcggcgccg gggccgacaa ggcgcggctg
     5821 gcggccctgc gccaacgcat cgaacaatcg cccgacgccc ataccgtgca ggcctacctg
     5881 cccctgtcgc aggcgcccac ctggcacgac ggccgcatcg tgccgcgcgc agccatggtg
     5941 cgcgtgttcg ccatggccga cggacagggc ggctggcacg tgctgccggg cgggctcacg
     6001 cgcatcgcca accgcgaaca gcaggtcgta tcgatgcagc gcggcggcag cagcatggac
     6061 acctgggtca ccaccgacgg ccccgtcgac ctgttctcga tgctgcccac gtcgatgcgg
     6121 cccgaggacg tcgcgcaggc gcgccggccg gtctccagcc gcgccgccga aaacctgttc
     6181 tggatgggcc gctacgccga gcgcctcggc aacgacgtgc gcctggccaa tgtcacgctg
     6241 acctggctca acggcgacct cgacaacccc gataccctgt cccgcgcgct ggccaggctg
     6301 tgcacgcgca gcggcctggt gccggccgag ctgccgcgct cgacccgcgt gttcgaacac
     6361 accctggtgg ccgagctcgc ctcgcgcggc acgcagcgcg gctttgcccg caacctctcg
     6421 tcgctgacct ttgccgccgg ccagatccgc gaccggctct cgcccgagca ctggcagctc
     6481 atcgtctcgg ccggcgcccg ctatcaccgc ctgatcgccg gcgacctcac cgacgacgcc
     6541 agccgctccg aacccgacga agacgcgccg ccgcgcaccg ccaccgacgt gctgcgcgcc
     6601 ctgcgccggc tcaaccggca aatcctttcc atcaccggcg cgcagaccga ccacatgacg
     6661 cgcgacgacg gctggcgcct gctcaccatc ggccgccata ccgagcgcct gtccaccatg
     6721 gccggcatgc tggccgccct gttcgaggac aacgccatcc tgcgcgacgg cggcttcaac
     6781 ctgctgctgc agctcttcga cagcgccatc acctaccgcg cctactacca gggccgctac
     6841 gagatccccg cgctgctcaa cctgctggtg ctcgacccca acaacccgcg ctcgccggcc
     6901 tgttcgctgg acgtgctgcg cgccacgctg acccggctgc ccggcagcgg cgccggcccg
     6961 gccaacgacc tgctggccct gctgcccacg cccgacctcg gcctgacgct gtcccagctg
     7021 tgccaccgcg acgccaacgg ccgctacacc gaggtgctgg cgctggcgcg ccgcctcgaa
     7081 gcggccgccg ccgcgctgtc cgatgaaatc agccggcgct atttcagcca ctcggccgac
     7141 gtcgaccaga gcctggccgc cgcctgaggg atccgccatg aacacgctcg agccccgccc
     7201 cctggccgtc gtccacgaga cccactacca ctacgacgcc cccgtcgagc tggcgcacca
     7261 cctgagctat ctgcgcccgc gcgacgatgc ccaccagacc gtcgaatccc acaccctgcg
     7321 catcagcccc acgcccaacg acgtgctgca aagcatggac gaccagggca actggcgcag
     7381 caccttcgcc ctgtcggccc cgcacgacga actggtcgtg cacgcctgca gccgcgtggt
     7441 cgtgcgcccg cgctatgccg ggctggatcc ggccgccggc atgccctggg aagccgcggc
     7501 cgaacgcctg cgctaccggg ccggcgctcc ctacgccccc gaggtcgaat acgccttccc
     7561 ctcgcccttc gtgccgctgc acgacgcgct gcgcgactat gccctggcct cgttcacgcc
     7621 ggaccggccg ctgcccgagg ccgccatcga cctgatgcat cgcatccacg ccgatttcac
     7681 ctatgcgccg gccagcaccc agatcgccac gccggtgctc gaggccttcg aggaaaagcg
     7741 cggcgtctgc caggactacg cccacgtcat gctgggctgc ctgcacacgc tgggcctggc
     7801 cgcgcgctat gtcagcggct acctggtgtc caagccgccg ccgggccagc cgcgcctgat
     7861 cggcgccgac gcctcgcacg cctggatctc ggtgcactgc ccgggcctgg cggccaatgg
     7921 cggctggctg gacctggacc ccaccaacga cctcatcgcg tccaccgagc acgtcaccct
     7981 ggcctacggc cgcgactatg gggacgtcac gccgctgcgc ggggtgatcc gcggcggcgg
     8041 cgggcacgag ctcaccgtgc acgtcaccgt gatgccctgg gatgaggcgc cggcagggct
     8101 gatgcaaggc ctggcacagt ttcaagactg atttattacg cagaataaaa ctttgtcacg
     8161 tgcgatacac atcgcaacct taagataccc agccacaccc cccggataac tgggcatgag
     8221 cgccgtactt ccctcctttc gtccccccga tgccctggta ataccccgct gtgcgggcga
     8281 tctctgccag gccatgccga tcatcgaagc ctcggactcc aacgcgacgg tcctgggcct
     8341 gttcacgacg caccgcaatc tgatcagcct gaccgtggtc gaggatgccc ggcccatcgg
     8401 cctgatcaac cgcgacatct tcctgtcgca gatgagcaag cccttccatc gcgagatcta
     8461 cgaccggaaa agctgcatcg cgttcatgga caaggaaccg ctcatcgtcg acgccggcac
     8521 gggcatcgaa gcgctcaccc tcaagaccat cgaatacggc gagaaggcct tgaccgacgg
     8581 cttcatcgtc acgcaagaag accgttttct cgggctgggc aacggcctgc aattgatgag
     8641 cgcggtggcc gccatccagg ccgagaaaaa ccgccagacc atgcagagca tcgaatacgc
     8701 cagcgtgatc cagcgcgcca tgctgcgcgc ctcgcgcgag gccctgaccg ccacgccgca
     8761 ccaggccgcg ctggtctggg agccgcgcga cgtggtcggc ggcgacttct accagttcgc
     8821 gtcccacccc gacggctggt tcggcgccgt ggccgactgt accggccacg gcgtcccggg
     8881 cgccttcatg accctgctcg cctcctcggc cctggccaag gcgctggaac agctcggtcc
     8941 gcgcgacccc gcggcgctgc tgtcggcggt caaccgcagc gtcaagacga tgctgggcca
     9001 gatgggcaag gccggcgagt cctccgagtc cgacgacggc atggatgcgg cattcttctg
     9061 gttcgatgcg cagcgccgcg aactgcgctt ttccggcgcg cgcatcgccg tgcacatcct
     9121 gcggcccggc gcgcaggcat tcgacgtgct ggcgggccag cgcatgggcg tgggctatgt
     9181 cgacagcgac gccgattacg tctgggcgca gaagaccgcc ccgctgccgc ccggcagcct
     9241 gctgttcatc accaccgacg gctataccga ccagatcggc ggcccacgca agatcgccta
     9301 cggcaagcgc cgcgcccacg acgtcatcct ggcctgccgc gacgaacccg tcggcgtcgt
     9361 ttgcgaaaac ctgacccagg ccctggcgca gtggcaaggc acccagcccc ggcgcgacga
     9421 catcacccta ttttgtaccc gcatcgagga acaccgatga acatcgagca ttccatgggg
     9481 caggaccgcg tatttctgca attggcccgc agccgcaacg tcctcttcta ccacaaggga
     9541 tatttctcgc agaacatcat cgcggccatg ggcgaggtcg tgcggctgca gctcgaggtc
     9601 gccggcgtgg aaggaccgac gcgccgcaag ctgttttcgt cctttgtcga gctggcgcag
     9661 aacatcatgc attattcgtc ggacacgatt ccccccgact ctgccgacag cagcgcgcgc
     9721 gcgggcgtcg tgtgcatcgc cgccgaagat ggccatcacg tcatgctgtg catcaacccg
     9781 atcgccacgg ccagcgtgac cgccctgcgc gacaagctcg aaccattgcg tgacatgtcg
     9841 ctggaacaga tcaagcaggc ctacaaggca tcgctgcgcg ccaacacccc cgaagacagc
     9901 aaaggcgccg gactgggctt tctgaccatg gcccgcgacg cgagcgcgcc gctggacttc
     9961 gccttccagc cccgcgacga cgctcccggc accacgcagt tctatctaaa agccattatc
    10021 taaataaacg ataagataga ctccccatca tggaaaatct ctatatcgct gccacggcca
    10081 cgtctcccga agtcgatttc cgcttcgagc agcaccagct atcgctcacc ggcgagtcct
    10141 acccggaaaa cgcggcggcc ttctacacgc cgatcatcga acgcctgcgc gcctacctgg
    10201 ccggatgcga cgacacggcc gtcaccgtcg acgtgaccct ggcctatttc aacagctcga
    10261 gcaccaagat gctgttcagc atcttcgatg cgctggacca ggccgcgcaa tcgggcaacc
    10321 gcgtggtcct gaactggcat tacgacgcag aggacgaaac catccttga
//
LOCUS       DASWPO010000319        49962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_344191, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000319 DASWPO010000000
VERSION     DASWPO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 49962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49962
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344191"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /gene="ilvD"
                     /locus_tag="VGC69_16580"
     CDS             <1..1350
                     /gene="ilvD"
                     /locus_tag="VGC69_16580"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY0296947.1"
                     /translation="MIKAADPKVTDAEAEMVERSACPTCGSCSGMFTANSMNCLTEAI
                     GLALPGNGTIVATHAWRRGLFEQAGRLVVDLCRRYYEQDDASVLPRSIATKSAFVNAM
                     TLDVAMGGSTNTVLHLLAAAQEAGVDFAMADIDKISRRVPCLCKAAPATDKYHIEDVH
                     RAGGILGILGELARADLLDLSCGNVHSGTLGNAIAAWDVGGGAGEAAQKFYRAAPGGI
                     PTTVAFSQDATFLTLDLDRKSGCIRSKENAYSKDGGLAVLYGNLAEKGCIVKTAGVDE
                     SQWTFTGRARVFESQDDAVEGILGDQVSAGDVVIIRYEGPKGGPGMQEMLYPTSYLKS
                     KGLGKTCALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEDGDTIEIDIPNRRIHLA
                     VSEADLARRRAAMQARGDRAWQPENRQRVVSLALQAYAALATSADRGAVRDLSQLKR"
     gene            complement(1340..2146)
                     /locus_tag="VGC69_16585"
     CDS             complement(1340..2146)
                     /locus_tag="VGC69_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296948.1"
                     /translation="MKGGFAANRSCQTKDFTMTKILSGKVALVTGGSRGLGAAIAAAL
                     ADQGADIAISYVASEEKAAAVVESLKAKGVRAIAIQADQGDPSASEPLVRTVAETLGK
                     LDVLVNNAAVAWQGKTIDDPGIDNAAMDRQWSINTMGVIANIRAAAKVLPEGGRIISV
                     GSGLGTRIAFPGTADYAASKAAVVGYSKGAARDLGARNITVNVVQAGMMDTDMAAGSK
                     DKLPPIIMESHAIHRYAAVEEVAAAIVFLAGPDAGFITGSVIDVNGGFTA"
     gene            complement(2143..2922)
                     /locus_tag="VGC69_16590"
     CDS             complement(2143..2922)
                     /locus_tag="VGC69_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0296949.1"
                     /translation="MANDAVPSQTSLSGKIALVTGGARGLGLETSRQLASLGAHVIVG
                     ARDGKKADDAAAALRDQGFEASALRLDITRETDRQAAHASIDKAHGKLDILVNNAGIL
                     LDSPDGGTPAPLQPSAALPGVVRDTFEANFFAPVFLTQILLPLIRRSAAGRVVNVSSI
                     RGSLAHLSDPTSPVYPIRALGYDTSKAALNAFTILIAEELRGTPIKINAIHPGWLRTA
                     MGGEQANMSVEEGARMAVKYASLGADGPTGGFFFLDERLPW"
     gene            3055..3639
                     /locus_tag="VGC69_16595"
     CDS             3055..3639
                     /locus_tag="VGC69_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0296950.1"
                     /translation="MGRHRQFDEGEALEAALSVFWQKGYEGASFEDLTKATGVARPGL
                     YAAFGNKEEIFRKALDLYDSQYMGFMADALNEPTSHGVVARILRGSAALHTMDSAHPG
                     CLGINGALACSDDAEPIRRELIRRRAATQLALRRRLDRAQREGDLPPSADSASLASFV
                     MAVSQGMAVQAKAGASRKTLEGLVAHVLDTWPSP"
     gene            complement(3631..4890)
                     /locus_tag="VGC69_16600"
     CDS             complement(3631..4890)
                     /locus_tag="VGC69_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0296951.1"
                     /translation="MPPLTLAAGAAMTADPAVAAAPSWVATWNASPQPAWGPDFLFPS
                     NVPGVLHDQTVRQIARVSLGGQRLRIVLSNAYGKQPLTVGKASVARPRKDGAMTANSS
                     RTVTFGGRETATLLPGAALVSDPVALAVPALAQVAVSLYLPNATPIDTFHWDGRQTGW
                     IVSGDQAADPALRTADGAAQSTTARLLLAGIQVETRQAARAVAVIGDSITDGAAASLD
                     QDKRWPDFLAARLAPHGVAVVNAGISGARLLSDGMGVNALARLERDVLAQPGVQSVVV
                     MLGINDISWPGTAFARESRRPAREALAAGYRQLVEQARSRGVRVIGATLAPFEGALSG
                     TPLDDYYNLDKDALRRQVNDWIRHGGAFDAVIDFDAALRDPDHPARIALRFDSGDHLH
                     PGDEGNRAMAQAVDLETLLPGLGMTSR"
     gene            5053..5964
                     /locus_tag="VGC69_16605"
     CDS             5053..5964
                     /locus_tag="VGC69_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0296952.1"
                     /translation="MARLEVNRSGELEVFVRVIELGGFSAAARACGMTPSAVSKLVAR
                     LEQRLGTRLVNRSTRQLQLTPEGCVFYERGVRILADLEEAERCAGEHTAPRGRLRVNA
                     NVPFGHHFLLPLVPEFLERHPDVTLDIVLTDEVIDILEQRTDVAVRAGPLKSSSLVAR
                     KLGQTRMVIVGAPAYLARHGTPATPEDLLEHNRLGANYARAQPGWPLRRGGEDMVLAV
                     TGNAQASDGEALHRLALAGLGLARLAAFQVREDIAAGRLSPVLEDCNPGDVEAVHAVY
                     VGQGGYLPQRVRAFLGFLAERVDIGQA"
     gene            6095..6475
                     /locus_tag="VGC69_16610"
     CDS             6095..6475
                     /locus_tag="VGC69_16610"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0296953.1"
                     /translation="MDRWTKSNCPICYGLDLLGDKWTLLIVRDMLTRQKRYYKEFLSS
                     DEGIATNILADRLKRMAEHGLVVREDDPDNKGQIVYRPTEKAQALQPVLDAMAQWGIT
                     HGPSALQVPSDMKRKKRNDSATRQ"
     gene            6453..7259
                     /locus_tag="VGC69_16615"
     CDS             6453..7259
                     /locus_tag="VGC69_16615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0296954.1"
                     /translation="MTQQQGSKGYALITGASSGIGAIYADRLARRGYDLILVARREDR
                     LRELAGRITAGTGRAVETVAADLGERASLAKVEAILRDDARITLLVNNAGTASIQPLL
                     AAEVDEMSDMIALNVDALTRLSYAAAPGFVRRGSGTLINIASVVAIAPEVLNGVYGAS
                     KAYVLAFSRSLRSELADKGVRVQVVLPGATATDIWANAGRRLEDMPKEILMSTDQMVD
                     AALAGLDLGEFATIPALPELADWDKFEQARQVLMPNLSLAEPAARYRQGA"
     gene            complement(7291..8133)
                     /locus_tag="VGC69_16620"
     CDS             complement(7291..8133)
                     /locus_tag="VGC69_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0296955.1"
                     /translation="MTDRIPAKPLGRQGATIPALGLGVFRMPGEDTARMVKTSLDLGY
                     RLIDTAQIYGNEADVGRGVAESGVARDQLFITTKVWVDNYRAEDLLRSTQESLARLRT
                     DYVDLLLLHWPGDQAPLAEQIEALDETAARGWARHIGVSNFNIGLMRQASALSKTPLV
                     NNQVEYHPYLSQDKVLAEAGRLGMTVTAYFAMADGKVLADPVIAGIARRLGKTPAQIA
                     LRWLVQQDNVVTLTKTLQTERARANMQVFDFALPAEDMHALSTLARPDGRIVSPDGLA
                     PAWD"
     gene            complement(8218..9378)
                     /locus_tag="VGC69_16625"
     CDS             complement(8218..9378)
                     /locus_tag="VGC69_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0296956.1"
                     /translation="MPIALWALAVGAFGIGTTEFVIMGLLPQVGADLGVSLSSAGLLV
                     TGYALGVTVGAPPVAILTTHLPRKTLLLGLMVIFTLGNLACALAPGYGTLMAARVLTS
                     LAHGAFFGVGSVVAANLVKPDKQASAIALMFTGLTLANVLGVPFGTWLGQAWGWRSTF
                     WAVSVVGLAALAAIAWLVPADHGAHQGNLRGELRALGRPQVLLGFLMTVLGFGGVFTA
                     FTYIAPMLTELSGFSPGAVSPILLLFGVGLVLGNTYGGKLADRRLMPTLVGSLALLAI
                     VLAVFAFTVHGKVAAVITVAVLGAAAFATVPPLQMRVLAKAGGAPNLASAFNIAAFNL
                     GNAAGAWLGGVTIDHGPGLGATPLAAAAVTAAGLLVALYSWRLDRHAAPAAA"
     gene            9479..10360
                     /locus_tag="VGC69_16630"
     CDS             9479..10360
                     /locus_tag="VGC69_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0296957.1"
                     /translation="MSLNSDALRMFLAVVDTGGMSAAAESLGQTASGVSRSLSRLEEQ
                     LGVTLLTRTTRRMELTEEGRLFLERARSIVGAMDEAEECMRIRSQQPSGTLRVDASAP
                     VMLHCVVPHVAAFRERYPEIRLELTSNDRIVDLVEHRTDIALRAGALGDSTLHARPLT
                     ASRLHVVASPQYLARHGRPRTVAALAQYTLLGFTQPETLNQWPLRHAQGNSYAAQPAL
                     AASSGETIRGMALTGAGIACLSEFMLRGDLDAGRLVPLLETADNGYRQPLHAVYYRNT
                     QLARRIACFLDFLSERI"
     gene            complement(10368..10943)
                     /gene="slmA"
                     /locus_tag="VGC69_16635"
     CDS             complement(10368..10943)
                     /gene="slmA"
                     /locus_tag="VGC69_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEY0296958.1"
                     /translation="MATKPGERKIQILHTLAEMLEQPHAARVTTAALAARMQVSEAAL
                     YRHFASKAQMFEGLIEFIETTIFTLINQIAAAEPCGLVQARKTTTMLLAFSERNRGMT
                     RVLTGDALVTEDNRLQERINHINDRIEATFKQCLRTAVSDGRLPPDTPVAAQASLLTH
                     LVLGRWLRYTQSGWKVMPTVHLDEQLRVALG"
     gene            complement(10992..11744)
                     /locus_tag="VGC69_16640"
     CDS             complement(10992..11744)
                     /locus_tag="VGC69_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY0296959.1"
                     /translation="MQHAHRLVARPAARVRRSRQEVTGAAPQRLWLFDLDNTLHDCSH
                     AIFPRIDQGMTRAVAETLGVDTATANELRRKYWKRYGATMIGLVRHHGVNAGDFLRRS
                     HDFDVAPLVRAERGLPACLRALPGRKVLLTNAPLHYAKAVLRHLGLLHHFDSLWGIEE
                     MRLHGEFRPKPSAALLRYVLAREGTPAARAVLVEDTLANLRSARRVGLRTVHVYHPGT
                     PFSKGDFQRPAYVDLRVNSVRDLLLRRRPLRG"
     gene            complement(11751..12653)
                     /gene="argB"
                     /locus_tag="VGC69_16645"
     CDS             complement(11751..12653)
                     /gene="argB"
                     /locus_tag="VGC69_16645"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0296960.1"
                     /translation="MTDTPDPAVVSSPAVKAAVLSEALPYLRRFHGKTIVVKYGGNAM
                     TEERLQRSFAHDVVLLKLVGLNPVVVHGGGPQIDDALRRVGKQGTFIQGMRVTDADTM
                     EVVEWVLGGQVQQDIVMMINEAGGLAVGLTGKDGGLIRARKMRMPDKEKPGETLDIGF
                     VGEIARIEPAVVKALQDDQFIPVISPIGYGEDGQAYNINADVVAGEIAKVLGAEKLLM
                     MSNIPGVLDRDKNLLRELTSQKIDELIADGTISGGMIPKIAGSLEAAKNGVNAVHIVD
                     GRVPHCLLLELLTDQGVGTMISAS"
     gene            complement(12727..13218)
                     /locus_tag="VGC69_16650"
     CDS             complement(12727..13218)
                     /locus_tag="VGC69_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0296961.1"
                     /translation="MKKSLIAVFAALIVGIAGWFTWHPAQAAPDVAFTTLDGKTVKLA
                     DLRGKVVLVKFWATSCVTCNAQMPATIADYQHYEPKGYVTMAVAMSYDPPNYVQNYAQ
                     TRQLPFPVAMDSQGELAKAFGDVRLTPTAFLIDKQGHIVKRYLGEYNPAEFHAAIEKA
                     LAG"
     gene            complement(13215..13973)
                     /locus_tag="VGC69_16655"
     CDS             complement(13215..13973)
                     /locus_tag="VGC69_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0296962.1"
                     /translation="MMPSSTLRPRAVLRAALLAAALAGTASGLSGCIPLLFGGAAAGT
                     ATVVTDRRSSGTQLDDQTIAFKVENQVSKQLGNSENTRINATSYEGRVLITGDVPTEA
                     AKSQATAAASQVEKVKSVANQLTVGPVESLGDRSNDSWLGSKVRTALLNAKYVPSGAI
                     TSTTDHSVVYLMGKVTQEEGNYAAAAVSGVGGVAKVVKLFTYISRDEAIRLSGNTTTS
                     APDSTSAAPATTAPIENGDDAAGGNDSMQAMPIK"
     gene            complement(13970..14563)
                     /locus_tag="VGC69_16660"
     CDS             complement(13970..14563)
                     /locus_tag="VGC69_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEY0296963.1"
                     /translation="MNLTSRMTAHFRDAIGVVELSMNTLAEPLAAAVELMFTAVANNG
                     KILACGNGGSAADAQHFIAELVGRFERERLPLAGIALNTDTAILTAVGNDYGYEEIFG
                     RQVDALGQSGDVLVALSTSGNSPNVVRAIEAARDRDMHVVALTGKGGGLIGELIAAHD
                     VHLCVPNDRTMRIQEVHGLLLHALCDGIDALLLGDPE"
     gene            complement(14656..15156)
                     /locus_tag="VGC69_16665"
     CDS             complement(14656..15156)
                     /locus_tag="VGC69_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY0296964.1"
                     /translation="MSIPPDHDPAPPAGDLLAYALADAARQRRRKRRPRKPAPRKDDP
                     QLSPMQRRGLQFEDRALDLLVRAGLQPLARNLRAAAGEIDLIMRDGDTLVLVEVRARA
                     GTSHGGAAASIDPAKQRRLARAAALLLPALARRHWRGRTPAVRFDAVLFDGQNAQWLR
                     HAFMLE"
     gene            15199..16140
                     /gene="rsmI"
                     /locus_tag="VGC69_16670"
     CDS             15199..16140
                     /gene="rsmI"
                     /locus_tag="VGC69_16670"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY0296965.1"
                     /translation="MNEEVTPPGAPGDAWRRVAERVAAQHWPAPALYVVATPIGNLGD
                     LGLRAWQALARADVIAAEDTRASRTLMDAWGIATPLMAAHRHNEAEAAEAICARLAQG
                     QRVALVSDAGAPAVSDPGARIVRAARAAGHVVVPVPGPSAVITALMGSGVTSDENPAY
                     AFAGFAPPKAAARQRWLRRWCALPAPVLMFESPHRLAAMLADLLDVCGPDRRLTVARE
                     LTKRFEEISTMAMGETAAWLAADAHHAQGEFVLIVHEAEGAADEDVEDGAAGIQIDAL
                     LDALLESLSVRDAARVAAKITGQPRDKLYGKALARKP"
     gene            complement(16148..17104)
                     /locus_tag="VGC69_16675"
     CDS             complement(16148..17104)
                     /locus_tag="VGC69_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY0296966.1"
                     /translation="MRIARTGSLLAALLAVLVMAGCSTTGGNKSGEGGQRYGGGYYKD
                     DGPGSNTPSDAALDAIPDAVPRIEPLASGPNQPYVVFGERYVPDTAEKPFREQGLASW
                     YGRKFHGARTSNGERYDMYAMTAAHPTLPIPSYARITSKVNGRSVIVRVNDRGPFHSN
                     RIMDLSYTAAFKLGLIGPGSGEVVVERILPSEIRTMMAQGHIAPEDSQLTADEAFRPV
                     SMPLPQAPAPEPLPPAANAAPASAGAYYLQLGAFSKEANAQTLVSRINEQLGNMPQHA
                     VAAQIAQLYRVRIGPYDTREAALGDAPTVNAKTGILPSLIAP"
     gene            complement(17101..17982)
                     /gene="rapZ"
                     /locus_tag="VGC69_16680"
     CDS             complement(17101..17982)
                     /gene="rapZ"
                     /locus_tag="VGC69_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY0296967.1"
                     /translation="MFSVVLITGTSGSGKSVALRMLEDAGYTCIDNLPVRLLSEFIAT
                     ARNDATIERVAVAIDARTPGELAELPAVIASLTAVGTHVRVVFLDSDTNTLVQRYSES
                     RRRHPLTGRAARDGGKPSLTDCIALEREMLAPLREHAHVIDTSDATPGQLRTWVRDLV
                     EADLAPLVLTFESFAYKLGVPNSADLVFDMRCLPNPHYDPELRPLTGRDAPVAEWLAG
                     YPAVGEMIDDITRFITRWMPQYQQDTRSYLTVAIGCTGGQHRSVYVVEQLAKRFADHH
                     PLLVRHRMQHATAAGHA"
     gene            complement(18131..19057)
                     /gene="hprK"
                     /locus_tag="VGC69_16685"
     CDS             complement(18131..19057)
                     /gene="hprK"
                     /locus_tag="VGC69_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY0296968.1"
                     /translation="MLTVQELVDDNADNIPFQWIAGKDAADRAIPDDGMAAADLVGHL
                     NLIHPSRVQVFGPEELAYYTRFDLRRRMHHMDELLIGGVPAILLADGLEPPEDLVEQC
                     AQHQIPLLKTSVAAAQLIDLLRIYLGKKLAPTTTVHGVFMDVLGLGVLITGESGLGKS
                     ELALELITRGHGLVADDAVEFSRTAPNMIEGHCPLLLQNLLEVRGLGLLDIRTIFGET
                     SVRRKMRLKLIVHLVRASAQDKFERLPLHDLTQDMLGLPVRRVMLQVAAGRNLAVLVE
                     AAVRNTILKLRGIDTMGEFMERQAMAILQSSQ"
     gene            complement(19063..19518)
                     /locus_tag="VGC69_16690"
     CDS             complement(19063..19518)
                     /locus_tag="VGC69_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0296969.1"
                     /translation="MNHLSRILPPGNVVLDLNATSKKRAFEHAGLLFENHHGLARATV
                     YDSIFARERLGSTALGEGVAVPHGRIKGLQQALCAFFRLARPIAFDAPDGQPVFMLLC
                     LLVPENAAQQHLDILAELAHLMSNQALRAALATETDPVKVHRTLTTGQL"
     gene            complement(19845..20183)
                     /gene="raiA"
                     /locus_tag="VGC69_16695"
     CDS             complement(19845..20183)
                     /gene="raiA"
                     /locus_tag="VGC69_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY0296970.1"
                     /translation="MNLSICGRHLDVTPAIREYVMNKLARVLRHFDHVIDTQVMLSVE
                     PLRQKAEITMHLSGKDLHCEAADENLYAAIDLLVDKLDRQVLKHKDKVQNHVAESAKR
                     QVAAAPATAP"
     gene            complement(20446..21225)
                     /gene="lptB"
                     /locus_tag="VGC69_16700"
     CDS             complement(20446..21225)
                     /gene="lptB"
                     /locus_tag="VGC69_16700"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY0296971.1"
                     /translation="MTATPPPAVTPQSQGTLRAIGLRKTYNGRTVVQDVSLSVAGGEV
                     VGLLGPNGAGKTTSFYMIVGLVPADSGRIEIDGMTITAMPIHKRARVGLSYLPQEASV
                     FRRLTVEQNIRAVLELQLDAEGKPLTRTKIDENLESLLDELQIGHIRGNTAISLSGGE
                     RRRVEIARALATRPRFILLDEPFAGVDPIAVIEIQRIVRFLKARGIGVLITDHNVRET
                     LGICDRAYIISEGKVLTNGHPDEIVSDPAVRRVYLGEHFRM"
     gene            complement(21248..21901)
                     /gene="lptA"
                     /locus_tag="VGC69_16705"
     CDS             complement(21248..21901)
                     /gene="lptA"
                     /locus_tag="VGC69_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY0296972.1"
                     /translation="MTHQTAPILPRLLGALLLATAAASAQAQIQAQAPAQGQAKTAPA
                     DEPSTQVVSDTLHYDDNKRESIFTGNVVATRGQLNIYSDKLVVNEDKDGNQFGTATAD
                     KSKVVTLTEDRPETFERIEGKGLRAEYDGAKQQFDLIGQAVVIRYICGKPYDTIRGQR
                     VRYYQKTGVYEAQGGPDSAAPGGRVRSLAEPRAKTQAAMDACRAAQGEAKPAPAAKK"
     gene            complement(21898..22506)
                     /gene="lptC"
                     /locus_tag="VGC69_16710"
     CDS             complement(21898..22506)
                     /gene="lptC"
                     /locus_tag="VGC69_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY0296973.1"
                     /translation="MKDRFPALMALVLLVALVMGTWWAADYAQRAILTDPPRRLTHEM
                     DAWTRNFVMLRTDPAGKPINRLEGVYGEHFPDDDSYHVTTPRAVGQQEGNPITVGTAK
                     TAIMDHNSKRITMIGDAHVHRQPDANNDPLDAYSEQFIIMPDDDVVYTDLPARVTKGK
                     STMNGVGMRYDNKTRQLQVYSSSDVNLAPNETRKATNPTNKP"
     gene            complement(22512..23111)
                     /locus_tag="VGC69_16715"
     CDS             complement(22512..23111)
                     /locus_tag="VGC69_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY0296974.1"
                     /translation="MNSPKLAHPVEALVLARVAPAVRELAAAVRLMLFDVDGVLTDGS
                     LYYGENGESFKRFHALDGHGLKLLMQSGVKVALVTGRSGSIVGRRAAELGIADVLVGV
                     RDKGAALTELTQRHGISVNETGFMGDDIIDLPAMQRAGFAASVPNALDYVAQSAHWVS
                     SRPGGSGAVRECCDLLLAARGRLGSFLTTQPLFGAGAIQ"
     gene            complement(23132..24115)
                     /locus_tag="VGC69_16720"
     CDS             complement(23132..24115)
                     /locus_tag="VGC69_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY0296975.1"
                     /translation="MTAQFSPDEALASARRTLQTETQALQDLSTRLDASFGQAVRLLL
                     ECRGRVVVSGVGKTGHIARKIAATLASTGTPAFFVHAAEAVHGDLGMVTADDVMIALS
                     YSGTGAELLIIVPVVRRMGARLIAITGNAKSELAQLADVHLDASVAQEACPLSLAPTA
                     STTASLALGDALAVACMEARGFGKEDFARSHPGGALGRRLLTHVRDVMRHGPDLPTVP
                     EDAPLFRALEEISAKRMGMTAVVDSANHPVGIFTDGDLRRLIERQGDVRGLTVAAGMS
                     RKPHAIGPDALAAEAAQLMDDQRLNQMLVVDAQGLLLGALHIHDLMAAKVV"
     gene            24319..25539
                     /gene="purT"
                     /locus_tag="VGC69_16725"
     CDS             24319..25539
                     /gene="purT"
                     /locus_tag="VGC69_16725"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEY0296976.1"
                     /translation="MTAFPAPVLGTPLSPCATRVMLLGSGELGKEVIIALQRLGVEVI
                     AVDRYADAPGHQVAHRAHVVSMTDARALRQVIEAEQPHIIVPEIEAIATDLLVELEAQ
                     GVARVTPTARAAQLTMNREGIRRLAAETLGLPTSPYQFVDTEDALRAAIAASIGFPCV
                     IKPVMSSSGKGQSVIRDEAGIAAAWRHAQEGGRVGGGRAIVEGFIHFEYEITLLTVRA
                     RGAGGEIETRFCEPIGHKQVDGDYVESWQPHPMAPAALARARDIALAVTSQLGGLGIF
                     GVELFVAGDQVWFSEVSPRPHDTGMVTMITQAQNEFELHARALLGLPVDTALRQPGAS
                     SVIYGGVEARGVRFYNVAQALAEPGTDIRLFGKPESFVKRRMGVGLATALDVTQARAK
                     ARRVSSAIRISAEA"
     gene            25700..26245
                     /locus_tag="VGC69_16730"
     CDS             25700..26245
                     /locus_tag="VGC69_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HEY0296977.1"
                     /translation="MPLSPQTERFILHFGEMGSRWGVNRTVGQIYALLFLAPQPLCAD
                     DIAESLAVSRSNVSMSLKELQSWRLVKVARRVGDRRDYFETPQDVWEIFRVLAEEKRK
                     REIDPTMTLLRDTLLEPPAGESEVHAQQRMREMLELIELSTGWFDEVQRLPPETLRNL
                     MKLGSRVQKVLEFAGRLRGRE"
     gene            26287..27873
                     /locus_tag="VGC69_16735"
     CDS             26287..27873
                     /locus_tag="VGC69_16735"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0296978.1"
                     /translation="MIDLDVVTLSRLQFAATALYHFLFVPLTLGLSFLLAVMESVYVM
                     TGKPIWKRMTMFWGTLFGINFALGVGTGVVMEFQFGMNWSYYSHYVGDIFGAPLALEG
                     LSAFFLEATFVGLFFFGWNRMGKVAHLAVTWLVAFGTNLSALWILIANGWMQNPVGAV
                     FNPDTMRMEMVDFAAVLFNPVAQAKFVHTVSAGYVLGSMFVMSISAWFVLKGRHLDLA
                     RRSMAVAASFGLAASLSVVVLGDESGYLTGEHQKMKIAAMESMWDTESAPASFNLLAW
                     PNRAEHKNDFAIRVPYVMGLIGTRSLTEPLLGINDLVKRAEGRIRSGIVAYDALQKIR
                     ANPKDADAREVFSRIWPDLGYGLLVKRYQTDLSKATDAQITQAAWDTVPTVMPLFVMF
                     RVMVLVGFYLILFFALMFWMVSKDRLVHCPRTLKLALWSLPLPWIAIESGWFVAEYGR
                     QPWVIEGVLPTYYAASGLSMLDLAITLAVFVLLYTVLLIVGIKVMLHAIKAGPKDSPP
                     AFAGGGAGSAAGSVQAALDA"
     gene            27890..29035
                     /gene="cydB"
                     /locus_tag="VGC69_16740"
     CDS             27890..29035
                     /gene="cydB"
                     /locus_tag="VGC69_16740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0296979.1"
                     /translation="MENLIPLDYATLRVIWWVLLGALLIGFAVMDGFDAGVAALLPLV
                     GKTDAERRVVINVVGPVWEGNQVWLITAGGAIFAAWPMLYAASFSGFYLAMMLILLAL
                     ILRPVGFKYRSKFEGTAWRNTWDGLLCFSGVVIALVCGVAMGNVILGVPHGFDPDTMR
                     PVYTGMFYQLFTPFALLSGVLSVTMLAMHGAVMLAWRTEDPISSRARGWARLAALLTI
                     VLFVLGGFWVAYGVDGQVAGQIDMGAPSNPLIKTVAVQAGAWMANFHKWPLMWIAPIL
                     GVAGALLVLLMPGAGALAFIASAATIAGVILTVGFALFPFLLPSSSQPAAGLTIWDGS
                     SSHLTLWIMLVAVVIFLPIITIYTAWVYRVMRGKVTHESVGDTPNSY"
     gene            29053..29172
                     /gene="cydX"
                     /locus_tag="VGC69_16745"
     CDS             29053..29172
                     /gene="cydX"
                     /locus_tag="VGC69_16745"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEY0296980.1"
                     /translation="MWYFSWILGLGLACTFGILNAMWFELREAKRQGPATTDQ"
     gene            29169..30905
                     /gene="cydD"
                     /locus_tag="VGC69_16750"
     CDS             29169..30905
                     /gene="cydD"
                     /locus_tag="VGC69_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEY0296981.1"
                     /translation="MSGVSGIEHTPQTASKAQSAWLSGQARLARAPLALAGAAPLAAG
                     ALLALQAWLLAGVLDAALAHGADRDQLLRPLVAIVALILLRAALAWVAERAGARAAEA
                     IKQRVRLALFDRLLRAGPQWTRRQVSGELASALVEQVEALDGFFAKYLPAVGAAAVLP
                     VAFSVLLMPQSVVAGLLLFITAPLIPLFMALVGWGAEAASRRHARTTARLSGFFADRL
                     RGLATLKLYGRADAAAADVMQASDALRVRTLAVLRVAFLSSAVLEFFAALGVAGVAVY
                     IGLRYLGMLGTPDPGFGLKAGFFCLLMAPEVYAPLRQFAAHYHDRAAARAAVAEIDRL
                     FDGLPGTGELEWTVAPPAAIDGASPALGLAVQGLTVEAPGRPAAVLHEVSLRLAAGEH
                     AVLMGASGAGKTTLLETVCGLRTAHGEILLDGKPLRDWDETALRAGVALIGQKPYLFQ
                     GTIADNIRFAKPGASDAEVAEAARRACVGEFAQSLPQGLDTPLGPRGVGLSGGQAQRV
                     ALARLFLREAGLVLLDEPTAHLDAATQDRVLDAVFAYARGRSLLLATHSHEVARRFAR
                     RLHVADGKVRDA"
     gene            30902..32599
                     /gene="cydC"
                     /locus_tag="VGC69_16755"
     CDS             30902..32599
                     /gene="cydC"
                     /locus_tag="VGC69_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEY0296982.1"
                     /translation="MSRRPRVSIVALYRRQRAGLLLALLLALLAVAAGVGLLGVSGWF
                     LAGAALAGAGSEFNLFAPSALVRGLSFLRIGTRYAERLAGHGATLHLLADLRGAVFGA
                     LVRLTPRQLARFRGGDLVARLTGDVDALDTVFLFVLAPIATALLGGLALAIVVACSLP
                     LAGLVLLLCLLSTCLLLPVGLALAARRSGAAVQQGAADLRTAVLDAVEGHADIAAMGV
                     QAAVLRHADGLCRRHGRARQSQSRLAAHGQWVLQMAAGLAVLAVLWTGLPAIAAGRLS
                     GPYLAGLLLAVLGIFEVAGPVMRGASRLGAARAAARRIAGLLDAEPDLRDPARPLPMP
                     ASGSLTMQGVRYAYPPAPGADAAPAWALDGIDLAVMPGERVAITGPSGSGKSTLLHLL
                     LRLDDPAAGEVRYGGCDLRQCAQADVHRRIALLAQDAPVFLGTVRSNLLIGDAQADEA
                     ALWSALQAARLDTFVRGLPRGLDSWVGETGAALSAGQARRLCLARAVLAPAAVLLLDE
                     PTAGLDAATEADFLRDLPQALAGRTVVLVTHAALPHGAVHRRLRMAGGRLEVLPETV"
     gene            32668..33216
                     /locus_tag="VGC69_16760"
     CDS             32668..33216
                     /locus_tag="VGC69_16760"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY0296983.1"
                     /translation="MQTDYADLIRRTIRSVPDWPEAGVVFRDITPVLQDPRTFRALID
                     LFVYRYMRQRVDLIACVDARGFIIGSALAYALNVGFVPIRKHGKLPCRTVSESYTLEY
                     GTAEVEMHADAVHTGQRVVLIDDLVATGGTMLAAIKLLQRLGANVLEAATIADLPDLG
                     GSEKIKATGTPFYAICSFTGKD"
     gene            complement(33239..34015)
                     /locus_tag="VGC69_16765"
     CDS             complement(33239..34015)
                     /locus_tag="VGC69_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0296984.1"
                     /translation="MDDIAPAASLTAHPWTLAFAGLAILASGTMRGFSGFGAALFGMP
                     ALSMVFAPALAAPIMTGLQILSSLQTVRHDRPHILWRQAIILSLVSSLAALAGARLLV
                     AVDPSTSRLVMGCIVLATVVFLASGWRYSGQPRLGLTLSVGACSGLGNGFAAMGGPPL
                     IAYFLGGPFSALAARATMTVIFAVQSTVSLLTLAALGSVSMRTLLYMAATYPLQAAGI
                     LLGQRLFLRHGDTHYRRVCVLILALLALLLIGRSAWSMLA"
     gene            complement(34029..34793)
                     /locus_tag="VGC69_16770"
     CDS             complement(34029..34793)
                     /locus_tag="VGC69_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0296985.1"
                     /translation="MKKVVFITGATSGFGRATARRFAAGGWSLVLTGRRTDRLEELQK
                     ELSPKVPVHIATLDVRDARAVQAAIDALPAEFRPVRSLINNAGLALAPVPSQNVELQD
                     WHTMIDTNVTGLVNVTHAVLPMLLETGAGASIVNLGSVAGQWPYPGGHVYGATKAFVQ
                     QFSYNLRCDLLSTGVRVTDIAPGMAETEFTLVRTRGDQSASDKLYRSTTPLTAEDIAE
                     QIFYVATLPDHININRLEIMPTRQAWSAFAVDRDKA"
     gene            complement(34827..36335)
                     /gene="ilvA"
                     /locus_tag="VGC69_16775"
     CDS             complement(34827..36335)
                     /gene="ilvA"
                     /locus_tag="VGC69_16775"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY0296986.1"
                     /translation="MSIDYLKRILTSKVYDVAVESPLEPAALLSQRISNTVLLKREDT
                     QAVFSFKLRGAYNKMANLAPASRNRGVIAASAGNHAQGVALSAKRLGCRAVIVMPTTS
                     PQVKIDAVKRLGGEVVLAGESFTDAYEHALLLEKSEKLTFVHPFDDPDVIAGQGTIGM
                     EILRQHPGNIDAIFVAVGGGGLIAGVAAYVKQLRPDIKIIGVQTEDSDAMLRSVRAGR
                     RVQLADVGLFSDGTAVKLVGAETFRLTRQYVDDFVVVDTDAICAAIKDVFQDTRSVLE
                     PAGALAVAGAKQYAAEHKLRGKTLVAIACGANMNFDRLRFVSDRAEVGEMREAVFAVT
                     MPEQRGSFRRFCELVGERSVTEFNYRMSDGDRAHVFVGLQVSAPAEPGKIAAHFRKNG
                     FDTLDLTHDEFAKTHLRHMVGGHSSQAAHEVLYRFEFPERPGALMRFLNAMNPGWNIS
                     LFHYRNQGADYGRILTGIQVPPGEKKQFKNFIAELGYPHWNETENPAYKLFL"
     gene            36462..37040
                     /locus_tag="VGC69_16780"
     CDS             36462..37040
                     /locus_tag="VGC69_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0296987.1"
                     /translation="MVHSLLVFVHKLLGLLALPKVGLPAIFLVSLISSSVLPVATEPV
                     VFGYIKLSPGMFWPGVLAATAGSILGGIILYFAGRGAHRAVQGLRQHRGPSGVSDGGE
                     MPDLAGRTIARHLKGRLAIRAQAWAHRLGPSALLLCWLPGLGDAMCLVAGWLRLPFWP
                     SLACMALAKLGRTLAVTVFLMWAAAHWPQWHL"
     gene            complement(37054..38061)
                     /locus_tag="VGC69_16785"
     CDS             complement(37054..38061)
                     /locus_tag="VGC69_16785"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0296988.1"
                     /translation="MNRPASPGWLDARPWLAGLPICDGLLPWTAPFLPSGADLLAQLA
                     RFRDAGIDHVSLTAAAGNDDLPCALSRLGYFRRVLAPARDWLAVAEDAAQIRQARASG
                     RFSVSFHFQTATPFCAGLDLVDAFRAAGIGRAILAYNEANPYADGCHESRNAGLTTLG
                     HRLLARMDASGMRVDLSHCGALTSLQALDAPLARVPVFSHSNARACFDHERNLTDEQI
                     RACGHRDSYIGISGVGMFLGARGAGIPQAMARHAAHIASLIGAAHVGLGLDFMYLEGS
                     DYGFFHAAADRWPRGYPPPPWDFLQPEQLGALVQALENVGFNQEEVRGILGENYLKRA
                     A"
     gene            complement(38058..39227)
                     /locus_tag="VGC69_16790"
     CDS             complement(38058..39227)
                     /locus_tag="VGC69_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0296989.1"
                     /translation="MRHHRPARLDYIAGIGVDRMGAVADGEARDWLRLENLDTDLPPC
                     AEALARTRAAIDQDAANSYLPFVGQDHLRATAARHVSTLTGVDYRPGNVVISAGGLSG
                     ILNVLLATIEVGDEVIVTDPTYAGLLNRIRLAGGIPRQVPLRFAPGEPWTLDREALRA
                     SITPKTRAMLLMSPSMPSGATLDAQDWAEVAALCVAHDLLLIVDSAMERLLFDGRTVL
                     HPAGLPGMAERCISVGAASKELRMIGWRVGWIVAPDALIPDIVAVSLANVVVPVGIAQ
                     EAAAIALQRSVDDLPAYVAELQRRRDMLAGELDGLPFGLPAGGWSLLLRTSDFGMSGE
                     TLAQRLLEQGVCATAMTGWGKQHGADYIRFVYANEPAHRLAGLGSKVRAALETTT"
     gene            39288..39944
                     /locus_tag="VGC69_16795"
     CDS             39288..39944
                     /locus_tag="VGC69_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0296990.1"
                     /translation="MSSNVNTDRSLAGQVVQALAARIIAGDIAPGAWLRQDELAEEFK
                     TSHVPVREALRRLEAQGLVVMEPRRGVRAAPLDAASIIEVARMRAALEPLALRHALPR
                     FPQGQLARLRECLRAPGPDLDIAGLEAANQQFHTLMTESCGMPRLLATLTDLHQASSR
                     HLFAAWKWLDWRPRSDREHEEILAALQAGAVDDACARLADHILAAGTALAQALAQTGR
                     "
     gene            40152..44102
                     /locus_tag="VGC69_16800"
     CDS             40152..44102
                     /locus_tag="VGC69_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEY0296991.1"
                     /translation="MNAPLASQVLASAVPPAPGARLREIPYNYTSFSDREIVSLLLGE
                     DAWQLLCDLRGERRTGRSARMLYEVLGDIWVVNRNPYLQDDLLDNPKRRKLLIEALNH
                     RLREVDLRRTPAGEATDVQRDNRVVDLLVLARSAVLDFERRFDETAQMRQQVQRVLGR
                     CTARDNIKFDGLSRVAHVTDATDWRVEYPFVVLTPDSEEEIAPLVRACIELGLTIVPR
                     GGGTGYTGGAIPLTWKSAVINTEKFETLGPVEQQVLPGLTEPVATIFAGAGVVTKRVT
                     EAAEHAGFVFAVDPTSAEASCVGGNIAMNAGGKKAVLWGTALDNLAWWRMVDPDGNWL
                     EVTRLAHNMGKIHDVDVARFELKWFDGNGRPGEKLLKTEVLEIAGRVFRKEGLGKDVT
                     DKFLAGLPGVQKEGCDGLITSARWVLHRMPKHMRTVCMEFFGQARDAIPSIVEVKGYL
                     DGAGRASGAILAGLEHLDERYLRAVGYATKSKRGTLPKMVLIGDIVGDDENVVAAAAS
                     EVVRLANTRHGEGFVAVSPEARKKFWADRSRTAAISRHTNAFKINEDVVIPLPRMGEY
                     TDYIERFNIELSTRNKIRLLDALDAYLAEPLPLGKAASADDDASLTPEALDERAGQAV
                     ALIDAARRRWQWLLDNLDMPLAQALPELDGLGLAHLHAVLAERLARQPDARVFDVLQD
                     RTVRVSWKTEVLAGLAGLFPGDACKKIIDDLRAVHARVLKSRVFVALHMHAGDGNVHT
                     NIPVNSDDYDMLREANETVARIMQVARDLDGVISGEHGIGLTKYEYLTEAELLPFQDY
                     KRRVDPNGHFNAGKLMPGADLRHAWTPSFSLMGHESIIMQQSEIGEIAASVKNCLRCG
                     KCKPVCATHVPRANLLYSPRNKILATSLLVEAFLYEEQTRRGVSIKHWEEFEDVADHC
                     TVCHKCLSPCPVDIDFGDVSMNMRALLRRMGRKSFNPGTAAAMFFLNAKDPATINATR
                     RAMVGVGYKVQRAAHDFLSSFTRKQTAHPPATVGKPPLREQVVHFVNKRMPGGLPKQT
                     ARKLLDIEDADYVPIIRDPQATTADTEAVFYFPGCGSERLFSQVGLATQAMLWHAGVQ
                     TVLPPGYLCCGYPQRGNGMNDKAEKIITDNRVLFNRVANTLNYLDIKTVVVSCGTCYD
                     QLAGYEFEKIFPGCRLIDIHEYLLEKGIKLEGVTGQKYMYHDPCHTPMKLQDPMKTVK
                     ALVGDATIKSDRCCGESGTLAVTRPDISTQVRFRKQEEMQKGMEALGAGPGDQVKVLT
                     SCPSCLQGLSRYEEDTGAKADYIVVEMARHILGESWMEDYVKRANSGGIERVLV"
     gene            complement(44120..44884)
                     /locus_tag="VGC69_16805"
     CDS             complement(44120..44884)
                     /locus_tag="VGC69_16805"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0296992.1"
                     /translation="MKEDRYFVTALARGLDVLRCFTAERTELGSTDIARLLGLSQSTA
                     WRLCYTLQKAGYLVPGYAPERLRVSPGVLALGCEALSHSGIAEVALPHITDIANRFSA
                     SVSLAGRDHLDMRILARASAPTMLKLNFHIGSALSIGNSAVGSAYLAAVSDTEREAVL
                     RELEQTQPQLWAANRQYLEESRQTYLRHGYVLNLSRYHLDVNALGVAVVSPNGRHVMA
                     MNCGGASSVMTRDKLEGPVAEAMKALAADLSTMLMV"
     gene            45003..47171
                     /locus_tag="VGC69_16810"
     CDS             45003..47171
                     /locus_tag="VGC69_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY0296993.1"
                     /translation="MANTSVSAGVDPLSTGFPDLSRFFAPRRVALIGATEDLSKFGGR
                     CMRQTLDFGFSGALYAVNPKRDSIFGLPCHAAIADLPETPDHVGIVLPAQAVPQALEQ
                     CAARGVPFATVFSSGFGELGTPEGQAAQRRIAEIARAGGLRLMGPNCNGMINFVDGFA
                     LTSTATIRGPRRPAGDIGVVSQSGGAGQVNVMWRAQQSGLGISYQVSCGNAADLDLTD
                     YAYFMVESEATRVVVMLVERLADGPRLRRLAERAAELGKPIVMVKVGRTEAGSRAAAS
                     HTGAITGADEVCDAALRQLGIIRVDDCNELYETAMLLRQGRTVRGRGAAATSISGGNL
                     VMVADLGASVGIQWPEYAQGTRDKLAEQLPGFGSASNPTDLTASAIGQEGAYERAART
                     ILADPSVDVMIPVLTIAGAADVKAAARVAADSDKLVPILWTGCAADDPDLTPAALVAQ
                     GLAVYRDALPCLKAVRRAMDYAELQRRRGEPASGRPGGVDAQAARRLLEQADGPLSEH
                     RSKALLACYGIAAPQECLAGSVAQAVDFAAALDAPVVLKVQSPDIAHKTEAGVIRLGV
                     RGPVAVSAAYGEVIQAAHAYRPDARIEGVLVQEMVTQAQEMLVGVSRDPTFGPVITLA
                     LGGIYVEVIKDAVFRLAPLTPQEARRALGELRAFRLLEGARGQPAADIDALVDAVVRV
                     SWLAADLGEHISELDINPLAVLPAGRGVRMVDALVVPRRG"
     gene            47186..47893
                     /locus_tag="VGC69_16815"
     CDS             47186..47893
                     /locus_tag="VGC69_16815"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0296994.1"
                     /translation="MIETAMQPPVLWLTVSRPQAANALDAATHDALAAALREAARRPG
                     VGAVVLAAAGGKAYSAGADLREFSEWPAGRAALRRRELLLQTLEALLALGKPVVAAVQ
                     APAIGAGAMLALACDEIVMADGAWLGFPEAQHGMPTPMGAVLLARRARWPVVQRLLQA
                     GERCAAFEALQAGLADDCVAAGVLPARCQARAAALAAIPAHAYAANKAWLNRPLGHAL
                     AEAAAYATAHQASDPSH"
     gene            47911..49053
                     /locus_tag="VGC69_16820"
     CDS             47911..49053
                     /locus_tag="VGC69_16820"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0296995.1"
                     /translation="MQLEIQPELAEMRAAVRRFTQEKLEPIALAIDHTGEVPPGTLEL
                     LREQGYLGMRMPAAFGGGDFDLPTYCLVLEELSRSHRIFTLMLDATSGLNPIAIARFG
                     TPAQQARYLSGLCAGTLHASFALTEPEAGSDAQAIRTRAARENGGWTLNGRKHFISGA
                     HLADVVLVMAVTDPAKRGAGGITAFLVDKGTPGFAVTRIDTTIGSEPIKLAELSFTDC
                     RVPDEAVLGEVGQGFKVAMASLTSGRMGVACSCIGAADRLLEMSAAYARERQTFGKPL
                     AERQAIQWMLADSATELAMARSLTYETLRRLLAGENIGAAASMCKLAASEMVGRVADR
                     AVQIHGGMGLVRGFLVERFYRDVRHYRVGEGASEVQRMLIAREVLQ"
     gene            49050..>49962
                     /locus_tag="VGC69_16825"
     CDS             49050..>49962
                     /locus_tag="VGC69_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0296996.1"
                     /translation="MSAPAAAIVGIGESPVGRVPGLDTLGLQRAAALRALADAGLSPA
                     DVDGLLTTPIRVENWAMPCGNVAEGLGLRPHYLATLDVAGASGTAMVHHAAMAVATGQ
                     CRTVLCVGGQNLLSFSSSGAAVQKLADAGWAHPEFEAPYGPLVPTLYALAARRHMHEY
                     GTTHRQMAEVAVTMRRHAALNPNAHKREPLSVEDVMASRMITSPLRMLDCALVSDGAA
                     AFVVTTPERARDLGQPPVRLLGSGYGHSHTYIGDYRDVTTTGAVQSGRDAFAMAGLKP
                     ADIDVAQLYDCFTITVIVELEDLGFCAK"
BASE COUNT         7494 a        16265 c        17884 g         8319 t
ORIGIN      
        1 atgatcaagg cggccgaccc caaggtgacc gacgccgagg ccgagatggt cgagcgcagc
       61 gcctgcccca cctgcggctc gtgctcgggc atgttcacgg ccaactccat gaactgcctg
      121 accgaggcca tcggcctggc gctgccgggc aacggcacca tcgtggccac gcacgcctgg
      181 cgccgcggct tgttcgagca ggcgggccgc ctggtggtcg acctgtgccg ccgctactac
      241 gagcaggacg acgcctcggt gctgccgcgt tccatcgcca ccaagagcgc cttcgtcaat
      301 gccatgacgc tggacgtcgc catgggcggc tcgaccaata ccgtgctgca cctgctggcc
      361 gcggcgcagg aagccggcgt ggacttcgcc atggccgaca tcgacaagat ctcgcgccgc
      421 gtgccctgcc tgtgcaaggc cgcgcccgcc accgacaagt accacatcga ggacgtgcac
      481 cgcgccggcg gcatcctggg catcctgggc gaactggcgc gcgccgacct gctggatctt
      541 tcctgcggca acgtgcacag cggcacgctg ggcaatgcca tcgccgcctg ggatgtgggt
      601 ggcggcgccg gcgaagccgc gcagaagttc taccgcgcgg cgcccggcgg cattcccacc
      661 accgtggcct tcagccagga cgcgaccttc ctcacgctcg acctcgaccg caagtcgggc
      721 tgcatccgca gcaaggaaaa cgcctactcc aaggacggcg gcctggccgt gctgtacggc
      781 aacctggccg agaagggctg catcgtgaag acggccggcg tggacgaatc gcaatggacc
      841 ttcaccggcc gggcgcgcgt gttcgaaagc caggacgatg ccgtcgaagg catcctgggc
      901 gaccaggtgt cggcgggcga tgtggtcatc atccgctacg aaggccccaa gggcggcccc
      961 ggcatgcagg agatgctcta tcccacgtcc tatctgaagt ccaagggcct gggcaagacc
     1021 tgcgcgctgt tcaccgatgg ccgcttctcg ggcggctcgt cgggcctggt gatcggccat
     1081 gcctcgccgg aagcggccga aggcggcgcc atcggcctgg tcgaggacgg cgacaccatc
     1141 gagatcgaca tccccaaccg ccgcatccac ctggcggtgt ccgaggcgga tttggccagg
     1201 cgccgcgccg ccatgcaggc gcgcggcgat cgcgcctggc agccggaaaa ccgccagcgc
     1261 gtggtgtcgc tggcgctgca ggcctatgcc gccttggcca cctcggccga ccgcggcgcg
     1321 gtgcgcgatc tgtcgcagct caagcggtaa atccgccgtt cacgtcgatg accgacccgg
     1381 tgatgaagcc ggcgtcgggc cccgcgagga agacgatggc cgcggccact tcctcgacgg
     1441 ccgcgtagcg gtgaatggca tgcgattcca tgatgatggg cggcagcttg tctttcgaac
     1501 cggcggccat gtcggtatcc atcatgccgg cctgcacgac gttgacggtg atattgcgcg
     1561 ccccgagatc gcgcgccgcg cccttgctgt agccgaccac cgcggccttg ctcgcagcat
     1621 agtcagcggt gcccgggaag gcgatgcgtg tgccaaggcc cgacccgacc gagatgatcc
     1681 ggccgccttc cggcagcacc ttggcggccg cgcggatatt ggcgatcacg cccatggtgt
     1741 tgatggacca ctggcgatcc atcgccgcat tgtcgatgcc ggggtcgtcg atggtcttgc
     1801 cctgccaggc gacggcggcg ttgttgacca ggacgtccag cttgcccagg gtctcggcaa
     1861 ctgttcggac cagcggctcc gacgcggaag gatcgccctg gtcggcctgg atcgcgatgg
     1921 cgcgcacgcc cttcgccttg aggctttcga cgacggccgc cgccttttct tccgaagcga
     1981 catagctgat cgcgatatcc gcgccctgat cggccagcgc tgccgcgatc gccgcgccga
     2041 ggccgcgcga accgcccgtc acgagggcga ccttgcctga aagtatcttt gtcatggtga
     2101 aatccttggt ttgacaggaa cggtttgcgg caaagccgcc cttcaccagg gcaggcgttc
     2161 gtcgaggaag aagaagccgc cggtcgggcc gtctgcgccg aggctggcgt acttgacggc
     2221 catgcgcgcg ccctcttcga cgctcatgtt tgcctgctcg ccgcccatcg cggtccgtag
     2281 ccagcctggg tggatcgcgt tgatcttgat cggtgtgccg cgcagttctt cggcgatcag
     2341 gatggtgaag gcgttgagcg cggccttgga ggtgtcgtag ccgagcgccc gaatcggata
     2401 gacgggcgag gttggatcgg agaggtgcgc gagcgatccc ctgatgcttg acacgttcac
     2461 gacgcggccc gcggcggatc gccggatcag cgggagcagg atctgcgtta ggaagacagg
     2521 cgcgaagaag ttcgcctcga aggtgtcgcg caccacgccc ggcagggccg cgctaggttg
     2581 caaaggcgcc ggcgtcccgc catcgggact gtccagcagg atgccggcgt tgttgaccag
     2641 tatgtccagc ttgccgtgcg ccttgtcgat cgatgcatgg gccgcctgtc gatccgtctc
     2701 gcgtgtgatg tcgagccgta gcgcggatgc ctcaaagccc tggtcccgca gcgcagcggc
     2761 agcatcgtcc gcctttttcc cgtctcgcgc gcccacgatg acatgggcgc cgagcgacgc
     2821 gagctggcgg cttgtctcca ggccgaggcc gcgcgccccg ccggtcacga gtgcgatctt
     2881 gccggacagg gaggtttgcg atggcacggc gtcatttgcc atggtggcgc tccttcaaca
     2941 tgttctttga aaataaaata ctgatcgtta tgataacgaa cggtacttta ttgatgaacg
     3001 aggcgtcaag tgttttatac tgatcagtac tgaaattctt gccgcggatt cgccatgggg
     3061 cgccacagac aatttgatga aggcgaggcg cttgaagccg cgctgtccgt cttttggcag
     3121 aaggggtatg aaggcgcctc gttcgaggac ctgacaaagg ccaccggtgt ggctcgtccg
     3181 ggcctctacg ccgcgtttgg aaacaaagaa gagatctttc gcaaggccct tgatctctat
     3241 gattcccaat acatgggttt tatggcggac gcattgaacg agcccacctc gcatggcgtc
     3301 gtggcgcgca ttctgcgggg atcggcggct ttgcacacca tggactcggc ccatcccggc
     3361 tgcttgggaa taaacggcgc gcttgcctgt tcggatgatg ccgagcccat ccgccgtgaa
     3421 ctcattcgcc gccgcgccgc cacgcagctt gccctgcgtc gacgcctgga tcgggcgcaa
     3481 cgcgaagggg atctgccgcc atcggcggat agcgcaagcc tggcttcttt cgtgatggcc
     3541 gtctcgcaag ggatggcggt gcaggccaaa gcgggcgcgt ccaggaaaac gctcgaaggg
     3601 ctggtggccc acgtcctcga tacgtggcct tcaccgtgac gtcattccga ggccgggcag
     3661 caaggtttca agatcgacgg cttgggccat tgcccggttg ccttcgtctc cggggtggag
     3721 atggtcgccg gaatcgaagc gcaatgcgat gcgggccgga tgatcggggt cgcgcaaggc
     3781 cgcatcgaaa tcaatgacgg catcgaacgc gccaccgtgg cgtatccagt cgtttacctg
     3841 ccggcgcagg gcgtccttgt cgaggttgta gtagtcgtcc agcggcgtgc cggacaacgc
     3901 accctcgaac ggggcgagcg ttgcgccgat cacccggacc ccacggctgc gagcctgctc
     3961 gaccaactgt cgatagcccg ccgccagtgc ttcccgtgcc gggcggcgtg attcgcgcgc
     4021 gaaggcggtg cctggccagg aaatatcgtt gatgcccagc atcacgacga cgctctgcac
     4081 gcccggttgc gccagcacgt cgcgctccag ccgggccagc gcgttgacgc ccatgccatc
     4141 ggacagcagc cgggcgcccg agatgccggc attgacgacg gccaccccat gcggcgccag
     4201 gcgagctgcc aggaagtccg gccagcgctt gtcctggtcc agactggccg ccgcaccgtc
     4261 ggtgatggaa tcgccgatca ccgcgacggc gcgcgccgcc tgcctggttt cgacctggat
     4321 gcccgccagc agcaggcggg ccgtggtgct ctgcgcggcg ccgtccgccg ttcggagcgc
     4381 cgggtccgcg gcctggtcgc cagagacgat ccagccggtc tggcggccgt cccagtgaaa
     4441 ggtgtcgatc ggcgttgcat tcggcaggta gaggctcacc gctacctgag ccagtgcagg
     4501 aaccgccaag gccacgggat cgctgaccag cgccgcgccg gggaggaggg tcgcggtctc
     4561 ccggccgccg aatgtcacgg tgcgcgagct gttggcggtc atggcgccgt ccttgcgcgg
     4621 tcgcgcgacc gaggccttgc ccactgtgag cggttgcttg ccgtaggcat tggatagaac
     4681 gatgcgcaag cgctgtccac ccaggctgac acgtgcaatc tggcgcacgg tctggtcatg
     4741 cagcacgcct gggacattgc tgggaaacag aaaatccggt ccccaggctg gctgtgggct
     4801 ggcattccag gtcgccaccc aggaaggtgc ggcggcaacg gcgggatcgg cggtcatggc
     4861 cgcccctgcc gcgagcgtca ggggcggcat cgtgcaggcc gaggccgagg cagcgggaag
     4921 ggatttggcg cgtcttttca tgtgagtgaa ttcaatgtcg atctggagac gaatcatgag
     4981 ttcaaagaca aaattagaat agaccggccg tcagaacatc atttatgaat tgaactcacc
     5041 atggaggcga acatggcccg tctggaagtc aatcggtccg gcgaattgga agtgttcgtg
     5101 cgcgtcatcg agctgggcgg cttttccgct gccgcccgcg cttgcggcat gacgccctcg
     5161 gccgtcagca agctggtcgc acgcctggaa cagcgtctgg gcacgcgcct ggtgaaccgt
     5221 tccacccggc agctccagct tacgcccgag ggttgcgtct tctacgaacg cggcgtgcgc
     5281 atcctggccg acctggaaga agccgagcgt tgcgcgggcg aacacacggc ccctcgcggc
     5341 cggctgcgcg tgaatgccaa tgtgcccttc gggcaccact tcctgctgcc gctggttccc
     5401 gagttcctgg aacgccatcc ggatgtgacc ttggatatcg tgctgaccga tgaggtcatc
     5461 gacattctgg agcagcgcac cgatgtggcc gtgcgtgccg gtccgctgaa aagctcgagc
     5521 ctggtggcgc gcaagctggg ccagacgcgc atggtgatcg ttggcgcgcc cgcttatctg
     5581 gcgcgtcacg gcacgccggc tacgccggag gacctgcttg aacacaatcg cctgggcgcg
     5641 aactacgcgc gggcgcagcc cggctggccc ttgcggcgtg gcggagagga catggtgctt
     5701 gccgtgaccg gcaacgcgca ggccagcgat ggcgaagcgc tgcaccgcct ggccttggcc
     5761 gggttggggc tggcgcgtct ggctgccttc caggtgcgtg aggacatcgc tgccggccgg
     5821 ctgtcgccgg tgctcgaaga ctgcaatccg ggcgatgtcg aagccgtgca cgccgtctat
     5881 gtcggccagg gcggctacct gccgcaacgg gtgcgcgcct tcctgggctt tcttgccgaa
     5941 cgggtcgata tcggccaggc ctgatcctgc tcgatatggg cgccaggcaa cgggtttgtc
     6001 cattaacttg caaaatgcaa gttaactgcg tagtattcaa cttgcgtttt gcaagtaaac
     6061 taaccggaac ctcacgcaac cggacgtacc catcatggac cgttggacga aaagcaattg
     6121 ccccatctgc tacgggctcg atctgctcgg cgacaaatgg acgctgctga tcgtgcgcga
     6181 catgctgacc cgccagaagc gctactacaa ggagttcctg agctccgacg aaggtatcgc
     6241 gaccaatatc ctggcggacc ggctcaagcg catggccgag catggcctcg tcgtccggga
     6301 ggacgacccc gacaacaaag ggcagatcgt ctaccgcccc acggagaagg cgcaggcttt
     6361 gcagcccgtg ctcgatgcca tggcgcaatg ggggatcacg catggtccgt ccgcgctgca
     6421 ggttccttcc gacatgaaaa ggaagaaaag aaatgactca gcaacaaggc agtaaaggct
     6481 atgccctgat cacgggcgct tcatcgggca tcggcgcgat ctacgccgac cgcctcgccc
     6541 gccgcggcta cgacctcatc ctggtggcgc gccgcgaaga ccggctgcgc gaactggccg
     6601 gccgcatcac ggccgggacc ggccgcgcgg tcgaaaccgt ggcggccgac ttgggcgagc
     6661 gcgcgtcgct ggccaaggtc gaggccattc tgcgcgatga cgcgcgcatt accttgctgg
     6721 tcaacaatgc cggcactgcg tcgatccagc cgctgctggc cgccgaggtg gacgagatga
     6781 gcgacatgat cgcgctcaac gtcgacgcgc tgacgcgcct gagctatgcc gcggcgcccg
     6841 gcttcgtgcg gcgcggcagc ggcaccctta tcaatatcgc gtccgtcgtg gcgatcgcgc
     6901 cggaagtcct caacggcgtc tacggcgcca gcaaggccta tgtgctggcc ttcagccgtt
     6961 cgctgcgcag cgaactggcc gacaagggcg tgcgcgtgca ggtggtgctg ccgggcgcca
     7021 cggcgaccga catctgggcc aatgccggcc gcaggctgga agacatgccg aaagaaatcc
     7081 tgatgtccac cgaccagatg gtcgacgcgg ccctggccgg cctggacctg ggcgagttcg
     7141 ccaccatccc ggccttgccg gaactggccg attgggacaa gttcgagcag gcgcgccagg
     7201 tgctcatgcc caatctgtct ctcgccgaac cggccgcgcg ctatcgccaa ggggcgtgag
     7261 ccgggctccg gcctggcctg tgccgtgagc tcagtcccag gcgggcgcca gcccgtcggg
     7321 ggacacgatg cggccgtcgg gccgggccag tgtcgacaag gcatgcatgt cttcggccgg
     7381 cagcgcgaaa tcgaacacct gcatgttcgc gcgggcgcgc tcggtctgca gcgtcttggt
     7441 caaggtgacc acattgtcct gctgcaccag ccagcgcagc gcgatctgcg ccggcgtctt
     7501 gcccaggcgc cgggcgatgc cggcgatgac ggggtcggcc agcaccttgc cgtcggccat
     7561 ggcgaagtag gccgtcacgg tcatgcccag gcggccggct tcggccagca ccttgtcctg
     7621 gctcaggtag gggtggtatt cgacctggtt gttgaccagc ggcgtcttgc tcagcgcgct
     7681 ggcctgccgc atcaggccga tattgaagtt gcttacgccg atgtggcgcg cccagccgcg
     7741 cgcggcggtt tcatcgagcg cttcgatctg ctcggccagc ggcgcctggt cgccgggcca
     7801 gtgcaggagc agcaggtcga cgtagtcggt gcgcagcctg gccaggcttt cctgcgtgga
     7861 gcgcagcagg tcctcggcgc ggtagttgtc cacccagacc ttggtggtga tgaagagctg
     7921 gtcgcgggcc acgcccgatt cggccacgcc gcggccgacg tcggcctcgt tgccgtagat
     7981 ctgcgcggtg tcgatcaggc ggtagcccag gtccagcgag gttttgacca tgcgggcggt
     8041 gtcttcgccc ggcatgcgaa agacgcccag gcccagggcg gggatggtcg cgccctggcg
     8101 gcccaggggt ttggcgggaa tgcggtcggt catgcgggtt cctgaaaaaa tcgtgatatg
     8161 aataggcgcg gggcgggcgg cctgtcgcgg cgcgcccgtt cgcgggaaag gcgggtttta
     8221 tgcggcggct ggcgcggcgt ggcggtccag gcgccagctg tagagggcca cgagcaggcc
     8281 ggccgccgtg acggccgccg ccgccagcgg cgtggcgccc agcccggggc cgtggtcgat
     8341 ggtcacgccg cccagccagg cgccggccgc attgcccagg ttgaaggcgg cgatgttgaa
     8401 ggccgaggcc aggttggggg cgccgccggc tttggccagc acgcgcatct gcagcggcgg
     8461 cacggtggcg aaagccgccg cgcccagcac ggccacggtg atgacggcgg ccaccttgcc
     8521 gtgcacggtg aaggcgaaga ccgccagcac gatggccagc agggccaggc tgccgaccag
     8581 cgtcggcatc aggcggcggt cggccagctt gccgccgtag gtattgccca gcaccaggcc
     8641 cacgccgaac agcagcagga tgggcgagac ggcgcccggc gagaagccgg acaattcggt
     8701 gagcatcggg gcgatgtagg tgaaggccgt gaacacgccg ccgaagccca atacggtcat
     8761 gagaaagccc agcagcactt gcgggcggcc cagggcgcgc agttcgccgc gcaggttgcc
     8821 ctggtgcgcg ccgtggtcgg ccggcaccag ccacgcgatg gcggccagcg cggccaggcc
     8881 cacgacgctc acggcccaga aggtggagcg ccagccccag gcctggccca gccaggtgcc
     8941 gaagggcacg cccagcacgt tggccagcgt caggccggtg aacatcagcg cgatggccga
     9001 ggcctgcttg tcgggcttga cgaggttggc ggccaccacc gaccccacgc cgaaaaaggc
     9061 gccgtgcgcc agcgaggtca gcacgcgggc ggccatcagc gtgccgtagc ccggggccag
     9121 ggcgcaggcc aggttgccga gcgtgaaaat gaccatcagc cccagcagca gcgtcttgcg
     9181 cggcaggtgg gtggtgagga tggccacggg cggtgcgccc acggtgacgc ccaatgcata
     9241 gccggtgacc agcaggccgg ccgacgacaa ggaaacgccc aggtccgcgc cgacttgcgg
     9301 cagcagcccc atgatgacga attccgtcgt gccgatgccg aaggcgccaa cggccagcgc
     9361 ccaaagcgcg ataggcatgt cttgctccag aaggaaaata cggagccagc attgtgcgcc
     9421 gcaaaatata tgagaaaaat agccctaaaa tcaatttact tgtgatctgg aggcacatat
     9481 gtcgctcaac tccgatgccc tgcgcatgtt tctcgccgtg gtcgacaccg gcggcatgag
     9541 cgccgccgcc gaatccctgg gccagaccgc ctcgggcgtc agccggagcc tgtcgcggct
     9601 ggaagagcaa ttgggcgtga ccttgcttac gcgcaccacg cggcgcatgg agctgaccga
     9661 agagggccgc ttgttcctgg agcgggcgcg cagcatcgtg ggcgccatgg acgaagccga
     9721 agagtgcatg cgcatccgca gccagcagcc ttcgggcacg ctgcgcgtgg acgcgtcggc
     9781 gccggtgatg ctgcactgcg tggtgccgca tgtggcggcg tttcgcgagc gctatccgga
     9841 gatccggctc gaactcacca gcaacgaccg catcgtggat ctggtcgagc accgcaccga
     9901 cattgccctg cgcgccggag cgctgggcga ctccaccctg cacgcgcgtc ccctgaccgc
     9961 ttcgcgcctg catgtggtgg ccagcccgca gtacctggcc aggcacggcc ggccgcgcac
    10021 ggtggcggcg ctggcccagt acacgctgct gggttttacc cagcccgaga cgctcaacca
    10081 gtggcctttg cgccatgccc agggcaacag ctacgcggcg cagcccgcgc tggcggcctc
    10141 cagcggcgag accatccgcg gcatggcgct gacgggcgcc ggcatcgcct gcctgtcgga
    10201 gttcatgctg cgcggcgacc tggacgcggg ccggctggtg cctttgctgg agacggccga
    10261 caacggctac cggcaaccct tgcacgcggt gtactaccgc aacacgcaac tggcgcggcg
    10321 cattgcctgc tttctggatt tcctgtcgga acggatatga ggcgggctca gccgagcgcc
    10381 acgcgcaatt gctcgtccag gtgcaccgtg ggcatgacct tccagccgct ttgcgtataa
    10441 cgcagccagc ggcccagcac caggtgggtg agcaggctgg cctgcgcggc caccggcgta
    10501 tcgggcggca ggcggccgtc ggagaccgcg gtgcgcaggc actgcttgaa ggtggcctcg
    10561 atgcggtcgt tgatgtggtt gatgcgttcc tgcagccggt tgtcttcggt gacgagggca
    10621 tcgccggtaa gcacgcgcgt catgccgcgg ttgcgttcgg aaaaggccag cagcatggtc
    10681 gtggtcttgc gcgcctgcac caggccgcag ggttcggcgg ccgcgatctg gttgatcagc
    10741 gtgaagatcg tggtttcgat gaactcgatc aggccctcga acatctgggc cttgctggcg
    10801 aagtggcggt agagcgcggc ttcggacacc tgcatgcgcg cggccagcgc ggccgtggtg
    10861 acgcgcgccg cgtgcggttg ctcgagcatt tccgccagcg tgtgcaggat ctggattttt
    10921 cgttcgccgg gcttggtggc catgatcagg ttcaacgggt ggaagcggcc ttgggcaggg
    10981 acgcccaccg attacccgcg caagggccgt cgccgcagca gcaggtcgcg taccgagttt
    11041 acccgcaagt ccacataggc tgggcgctgg aaatccccct ttgaaaaggg ggtgccgggg
    11101 tggtagacat ggacggtgcg caggcccacg cggcgggccg agcgcaggtt ggcgagcgtg
    11161 tcttccacca gcacggcgcg cgcggcgggc gtgccttcgc gcgcgagcac ataccgcagc
    11221 agcgccgcgg agggcttggg ccggaactcg ccgtgcaggc gcatttcctc gatgccccac
    11281 aggctgtcga aatgatgcag caggcccagg tggcgcaata ccgccttggc atagtgcagc
    11341 ggcgcgttgg tgagcagcac cttgcgcccg ggcagggcgc gcaggcaggc gggcaggccg
    11401 cgttcggcgc gcaccagcgg cgccacgtcg aagtcgtggc tgcggcgcag gaagtcgccg
    11461 gcgttgacgc cgtggtggcg caccaggccg atcatggtgg cgccgtagcg cttccagtac
    11521 ttgcggcgca gttcattggc cgtggccgtg tccacgccca gcgtttcggc cacggcgcgg
    11581 gtcatgccct ggtcgatgcg cgggaagatg gcgtgcgagc agtcgtgcag cgtgttgtcg
    11641 agatcgaata gccacaggcg ctgcggcgcc gcgcccgtga cctcctggcg gctgcgccgc
    11701 acgcgtgccg cgggccgcgc cacgagtcgg tgtgcgtgtt gcatggcgcg tcaggacgcg
    11761 ctgatcatgg tgcccacgcc ctggtcggtg agcagttcca gcagcaggca gtgcggcacg
    11821 cggccgtcga cgatgtgcac ggcgttgacg ccgttcttgg cggcctcgag cgagccggcg
    11881 atcttgggga tcatgccgcc cgagatggtg ccgtcggcga tcagttcgtc gatcttttgc
    11941 gaggtgagct cgcgcagcag gttcttgtcg cggtcgagca cgccggggat gttggacatc
    12001 atcagcagtt tttcggcgcc cagcaccttg gcgatctcgc cggccaccac atcggcgttg
    12061 atgttgtagg cctggccgtc ctcgccgtag ccgatggggg agatcaccgg gatgaactgg
    12121 tcgtcctgca gcgccttgac cacggccggc tcgatgcggg cgatctcgcc gacaaagccg
    12181 atgtccagcg tctcgccggg tttttccttg tcgggcatgc gcatcttgcg cgcgcggatc
    12241 aggccgccgt ccttgcccgt gaggcccacg gccaggccgc cagcctcgtt gatcatcatg
    12301 acgatgtcct gctgcacctg gccgcccagc acccattcca ccacttccat ggtgtcggca
    12361 tcggtgacgc gcatgccctg gatgaaggtg ccctgcttgc cgacgcggcg cagcgcgtcg
    12421 tcgatctgcg ggccgccgcc gtgcaccacc accgggttca ggccgaccag cttgagcagc
    12481 accacgtcgt gggcgaagct gcgctgcagg cgctcttcgg tcatggcgtt gccgccgtat
    12541 ttcaccacga tggtcttgcc gtggaatcgt cggagatagg gcagcgcctc ggaaagcacg
    12601 gccgctttga cagcgggaga cgagacaacg gcagggtcgg gggtgtcggt catggtggcg
    12661 tggaaaaaac cgatgggaat gccgccggcc aggaggatgg gccggcggtg ctgcgcggat
    12721 tgtagatcaa ccggccaggg ctttttcgat ggctgcgtgg aattcggccg ggttgtattc
    12781 gcccaggtag cgcttgacga tgtgcccctg tttatcgatc aggaaagccg tgggcgtaag
    12841 ccgcacgtcg ccgaaggcct tggccagctc gccctggctg tccatcgcca cggggaaggg
    12901 cagttggcgc gtctgggcgt agttctgcac gtagttgggc gggtcgtagg acatggccac
    12961 ggccatggtg acgtagccct tgggttcgta gtgctggtag tcggcgatgg tggcgggcat
    13021 ttgcgcgttg caggtgacgc agctggtggc ccagaacttc accagcacga ccttgccgcg
    13081 caggtcggcg agtttgaccg tcttgccgtc gagcgtggtg aaggcgacat cgggcgcggc
    13141 ctgcgcgggg tgccaggtaa accagccggc tatccccacg atcagggcgg cgaatacggc
    13201 gatcagtgac tttttcattt gatgggcatg gcctgcatgg aatcgttgcc gccggccgcg
    13261 tcgtcgccat tctcgatggg cgcggtggtg gcgggcgcgg cgctggtgct gtcgggcgcc
    13321 gacgtggtgg tgttgccgga caggcggatg gcctcgtcgc ggctgatata ggtgaatagt
    13381 tttaccactt tggccacgcc gccgacaccc gatacggcgg cggcggcgta attaccttct
    13441 tcctgggtga ctttgcccat caggtagacc acgctgtggt cggtggtgct ggtgatggcg
    13501 cccgagggca cgtatttggc gttgagcagg gcggtgcgca ccttggaacc cagccaggag
    13561 tcgttgctgc ggtcgccgag cgactcgacg gggccgacgg tgagctggtt ggcgacggac
    13621 ttgaccttct cgacctggct ggcggcggcg gtggcctggc tcttggccgc ctcggtgggc
    13681 acgtcgccgg tgatcagcac gcggccttcg taggaggtgg cgttgatgcg cgtgttctcg
    13741 ctgttgccca gctgcttgga gacctggttt tcgaccttga aggcgatggt ctggtcgtcg
    13801 agctgggtgc ccgaggagcg gcggtcggtg accaccgtcg ccgtgccggc cgcggcgccg
    13861 ccgaagagca gcgggatgca gccgctcagg cccgaggcgg tgccggccag cgccgcggcc
    13921 agcagcgcgg cgcgcaggac ggcgcggggc cgcagggtgg aactgggcat cattcagggt
    13981 ctcctagcaa gagggcgtcg atgccgtcgc acagggcatg cagcagcagg ccatggacct
    14041 cctggatgcg catggtgcgg tcgttgggta cgcagagatg gacatcgtgc gcggcgatca
    14101 gttccccgat caggccgccg cccttgccgg tgagggcgac gacgtgcatg tcgcggtcgc
    14161 gcgcggcctc gatggcgcgc accacattgg gcgagttgcc gctggtggac agggccacca
    14221 gcacgtcgcc ggactggccc agggcgtcga cctggcggcc gaagatctct tcgtagccgt
    14281 agtcgttgcc cacggcggtg aggatggcgg tgtcggtgtt gagcgcgatg ccggccaggg
    14341 gcaggcgctc gcgctcgaag cggccgacca gttcggcaat gaagtgctgg gcgtcggcgg
    14401 ccgagccgcc gttgccgcag gccaggatct tgccgttgtt ggccacggcc gtgaacatca
    14461 gctcgaccgc ggcggccagg ggctcggcca gggtgttcat gctgagctcg acgacgccga
    14521 tggcgtcgcg gaaatgcgca gtcatccggg aggtcaggtt catggccgga ttatctcatg
    14581 ggggaggaag ggaatccttc ggggtgcaag cgggcatggc ggtaaatatt gcaagcgttt
    14641 gctgtgcccg ccgcctcatt ccagcatgaa ggcatgacgc agccattgcg cgttctggcc
    14701 gtcgaacagc acggcgtcga agcgcacggc cggcgtgcgg ccccgccagt gccggcgcgc
    14761 cagggcgggc agcagcaagg cggcggcgcg ggccaggcgg cgctgcttgg ccggatcgat
    14821 gctggccgcc gcgccgccgt ggctcgtgcc ggcccgggcg cgcacttcca ccagcaccag
    14881 ggtgtcgccg tcgcgcatga tcaggtcgat ctcgccggcc gctgcccgca ggttgcgcgc
    14941 cagcggctgc aggccggcgc gtaccagcag gtccagggcc cggtcttcga attgcaggcc
    15001 gcggcgctgc atgggcgaga gctgcgggtc gtccttgcgc ggcgcgggtt tgcgtgggcg
    15061 ccgcttgcgc cggcgctgcc gggcggcatc ggccagggca taggccagca ggtcgccggc
    15121 agggggcgcc ggatcgtggt cgggcgggat gctcatgcgg ctacactgac acggttttca
    15181 cagccgcggt gcgctgcaat gaacgaagaa gtcactccgc cgggggcgcc cggcgatgcc
    15241 tggcgccgcg tagccgagcg tgtcgcggcg cagcactggc cggcccccgc cctgtatgtg
    15301 gtcgccacgc ccatcggcaa cctgggcgac ctgggcctgc gggcctggca ggcgctggcc
    15361 cgggccgatg tgatcgccgc cgaggacacc cgcgccagcc gcaccttgat ggatgcctgg
    15421 ggcatcgcca cgcccctgat ggccgcgcat cgccacaacg aagccgaggc ggccgaggcc
    15481 atctgcgcgc gcctggccca ggggcagcgc gtggccctgg tgtccgatgc cggcgcgccc
    15541 gcggtgagcg acccgggcgc gcgcatcgtg cgcgccgcgc gcgcggccgg ccatgtcgtg
    15601 gtgccggtgc ccggccccag cgccgtgatc acggcgctga tgggcagcgg cgtcacttcc
    15661 gacgaaaacc ccgcctatgc cttcgccggt ttcgcgccgc ccaaggcggc cgcgcggcag
    15721 cgctggctgc gccgctggtg cgcgctgccg gcgccggtgc tgatgttcga gtcgccgcac
    15781 cgcctggcgg ccatgctggc cgacctgctg gacgtgtgcg gtcccgatcg ccggctcacc
    15841 gtggcgcgcg agctgaccaa gcgcttcgag gagatcagca ccatggcgat gggcgagacg
    15901 gccgcctggc tggccgccga tgcgcaccat gcccagggcg agttcgtgct catcgtgcac
    15961 gaggccgaag gcgcggcgga tgaggatgtg gaagacggcg ccgcgggcat tcagatcgac
    16021 gccctgctcg atgccctgct ggaaagcctg tcggtgcgcg atgccgcgcg cgtggcggcc
    16081 aagatcaccg gccagccgcg cgacaagctc tacggcaagg cgctggcgcg caagccctag
    16141 cctgggctca cggggcaatc aagctgggca ggatgcccgt cttggcattg accgttggcg
    16201 catcgcccag cgccgcctcg cgcgtgtcgt aggggccgat gcgcacgcga taaagctggg
    16261 cgatctgcgc ggccacggcg tgctggggca tattgcccag ttgttcgttg atccggctga
    16321 ccagcgtctg cgcattggct tccttgctga acgcgcccag ctgcaggtag taggcgccgg
    16381 ccgatgccgg cgcggcgttc gccgcgggcg gcagcggttc cggcgccggg gcctgcggca
    16441 gcggcatgct caccgggcgg aaggcctcgt cggcggtgag ctgggagtct tcgggggcga
    16501 tatgtccttg ggccatcatg gtgcggattt cgctgggcag gatgcgctcg accaccactt
    16561 cgccgctgcc cgggccgatc aatcccagct tgaaggccgc cgtgtaggac aggtccatga
    16621 tgcggttgct gtggaagggg ccgcggtcgt tgacccgcac gatcaccgag cggccgttga
    16681 ccttgctggt gatccgcgca tagctgggga tgggcagggt ggggtgcgcc gccgtcatgg
    16741 cgtacatgtc gtagcgctcg ccgttggacg tgcgggcgcc atggaatttg cggccgtacc
    16801 acgaggcgag gccctgctcc cggaaaggct tctcggcggt gtccggcaca tagcgttcgc
    16861 cgaacaccac atagggttgg ttgggtccgc tggccagcgg ctcgatgcgc ggcacggcat
    16921 cgggtatggc gtcgagggcg gcgtccgacg gcgtgttgct gcccgggccg tcgtccttgt
    16981 agtagccgcc gccgtagcgc tggccgcctt caccgctttt gttgccgcct gtcgtcgaac
    17041 aacccgccat gaccaggacg gccagcaagg cggccagaag cgatccggtg cgcgcgatcc
    17101 tcatgcgtgc ccggccgccg tggcatgctg catgcgatgg cgcacgagca agggatggtg
    17161 gtcggcgaag cgcttggcca gctgctccac cacgtacacc gaacggtgct ggccgcccgt
    17221 gcagccgatg gccacggtga gatagctgcg cgtgtcctgc tggtattgcg gcatccagcg
    17281 ggtgatgaag cgggtgatgt cgtcgatcat ttcgcccacg gccggatagc cggccagcca
    17341 ctcggcgacc ggcgcatcgc ggccggtcag cgggcgcagc tcggggtcgt agtgggggtt
    17401 gggcaggcag cgcatgtcga acaccaggtc ggcgctgttg ggcacgccca gcttgtaggc
    17461 gaaggattcg aaggtcagca ccagcggcgc gagatcggcc tcgacgaggt cgcgcaccca
    17521 ggtgcgcagc tgcccgggcg tggcgtcgga ggtgtcgatg acgtgggcgt gctcgcgcag
    17581 gggcgccagc atctcgcgct cgagcgcgat gcagtcggtc agcgaaggct tgccgccgtc
    17641 gcgcgcggcg cggccggtga gcggatggcg gcggcgcgat tcggaatagc gctgcaccag
    17701 cgtgttggtg tcggaatcga ggaacaccac ccgcacatgg gtgcccacgg cggtgagcga
    17761 ggcaatgacg gcgggcagct cggccagttc gcccggcgtg cgggcatcga tggccacggc
    17821 cacgcgttcg atggtggcgt cgttgcgcgc ggtggcgatg aattcgctca gcaggcgcac
    17881 gggcaggttg tcgatgcagg tatagccggc atcttcgagc atgcgcaagg cgacggactt
    17941 gccggaaccc gaggtgcccg tgatcaggac gacgctaaac atggatggcg cgtggcatga
    18001 gaaggtctca acggggctta tcttagctgt gaaaaggcgc cagaatattt cacgtttgca
    18061 aacgccgggc cgccccaagg caaaagcggc ccctggcggg gccgcaagcc gaaggcgcgg
    18121 cgcggggcgt ttactggctg ctttggagga tggccatcgc ctggcgctcc atgaactcgc
    18181 ccatggtgtc gatgccgcgc agcttgagga tggtgttgcg cacggcggcc tcgaccagca
    18241 cggccaggtt gcggccggcg gccacctgca gcatgaccct gcgcaccggc aggcccagca
    18301 tgtcctgcgt caggtcgtgc agcggcaggc gttcgaattt gtcctgcgcg ctggcgcgca
    18361 ccaggtgcac gatgagcttt aagcgcattt tgcgccgcac cgaggtctcg ccgaagatgg
    18421 tgcgtatgtc gagcaggccc aggccgcgca cttcgagcag gttctgcagc agcagcgggc
    18481 agtggccttc gatcatattg ggcgcggtgc gcgagaactc gacggcgtcg tcggccacca
    18541 ggccgtggcc gcgcgtgatc agttcgagcg ccagttcgct cttgcccagg cccgattcgc
    18601 cggtgatcag cacgcccagg cccagcacgt ccatgaacac gccgtgcacg gtggtggtgg
    18661 gcgcgagctt cttgcccagg tagatgcgca gcaggtcgat gagctgggcc gcggccaccg
    18721 aggtcttcag cagcgggatc tggtgctgcg cgcattgttc gaccaggtct tccggcggct
    18781 ccaggccgtc ggccaggagg atggccggca cgccgccgat gagcagctcg tccatgtggt
    18841 gcatgcggcg gcgcaggtcg aagcgcgtgt agtaggccag ctcttcgggc ccgaacacct
    18901 ggacgcgcga gggatggatc aggttgaggt ggcccaccag gtcggcggcg gccatgccgt
    18961 cgtcggggat ggcccggtcg gcggcgtctt tgcccgcgat ccactgaaac ggtatgttgt
    19021 cggcgttgtc gtcgaccagt tcctgcacgg taagcatggc ggtcagagtt gtcccgtggt
    19081 gagcgtgcga tgcaccttga ccggatcggt ctcggtggcc agggccgcgc gtaatgcctg
    19141 attggacatc aaatgcgcca gttcggccag aatgtccagg tgttgttgcg cggcattttc
    19201 cggcaccagc aggcacagca gcatgaaaac gggctggccg tcgggcgcgt cgaaggcaat
    19261 gggcctggcc agcctgaaga aggcgcacag cgcctgctgc aggcccttga tgcggccgtg
    19321 cggcacggcc acgccttcgc ccagcgccgt ggatccgagg cgctcgcggg cgaagatgct
    19381 gtcgtacacg gtggcgcggg ccaggccgtg gtggttttca aatagcaggc cggcgtgctc
    19441 gaatgcgcgt ttcttgctgg tggcgttcag gtcgagcacc acgttgccgg gagggagaat
    19501 gcgcgacaga tggttcatgc gcggcattat agggagagga tgacgcggtg caaaaaagcc
    19561 gggggacgga tcagctcggt cgtttgagtt cttgaggcgc cgaggctgcc ctgcggaatt
    19621 ccgatgctcg cccccggcgg gggctgtgct tcggattcct ccggggtcct cggcttgcaa
    19681 ggggatcatg cgacgagaaa aacccgcctc acaacgaaaa aagccacccg cgaggatggc
    19741 tttttgcttt tgccgccggg ccgccccaag gcaagaacgg ccccttcggg gggcagcaag
    19801 ccgaaggcgc ggcgtggggg gctttctttg cctgctgctt gcgcttatgg cgcggtggcg
    19861 ggggcagcgg cgacctgacg cttggccgat tcggccacgt ggttctgtac cttgtctttg
    19921 tgcttgagaa cctggcggtc gagcttgtcg acgagcaggt cgatggcggc gtagaggttc
    19981 tcgtcggctg cttcgcagtg gaggtccttg ccgctcagat gcatggtgat ttcggctttt
    20041 tgccgcaggg gctctaccga gagcatgacc tgggtgtcga tgacgtgatc gaaatgtcgc
    20101 aacacacgcg ccagtttgtt catgacatat tcccggatgg ccggggtgac gtcgagatgg
    20161 cgaccgcaaa tgctcaggtt catacgtgct ctcctacaaa aagggaaaaa gtgcgcggga
    20221 atgcgcttag gggtcgtaag gcgggctcct tcttggtggc aggggaaacg gtcggcgcgg
    20281 tggcgcccat gaacctagtg tagagacttt ggggcgtaaa acaaggcatg ccgttcacga
    20341 aattgtgacg gcgtgatgta catgggggac atgttttaag ggtgtacccg agacgggtcg
    20401 gcaagccccg gcagggcatg ccgcggggcg catcccgtgc ggaaattaca tgcggaaatg
    20461 ctcgcccagg tagacgcggc gcacggccgg gtcgctgacg atttcgtcgg ggtgcccgtt
    20521 ggtgagcacc ttgccttcgc tgatgatgta ggcgcggtcg cagatgccca gcgtttcgcg
    20581 cacattgtgg tcggtgatga gcacgccgat gccgcgcgcc ttgagaaagc gcacgatgcg
    20641 ctggatctcg atcacggcga tggggtccac gccggcgaag ggctcgtcga gcaggatgaa
    20701 gcgcggccgc gtggccaggg cgcgggcgat ttccacgcgg cggcgttcgc cgcccgacag
    20761 cgagatcgcc gtgttgccgc ggatgtggcc gatctgcagt tcgtcgagca gggattcgag
    20821 gttttcgtcg atcttggtgc gcgtcagcgg cttgccttcg gcgtcgagct gcagctcgag
    20881 caccgcgcgg atgttctgct cgaccgtcag gcggcggaac accgaggcct cctgcggcag
    20941 gtacgacagg cccacgcgcg cgcgcttgtg gatgggcatg gcggtgatgg tcatgccgtc
    21001 gatctcgatg cggccggagt cggcgggcac caggcccacg atcatgtaga aactggtggt
    21061 cttgcctgcg ccgttggggc cgagcaggcc cacgacctcg ccgccggcca cggacagcga
    21121 gacgtcctgc accacggtgc ggccgttgta ggtcttgcgc aggccgatgg cgcgcagcgt
    21181 gccttgcgat tgcggcgtca cggcgggcgg gggagtagcg gtcatgtcgt ctgggatatc
    21241 cgtcgggtta cttcttggcg gccggcgcgg gcttggcctc gccctgggcg gcgcggcagg
    21301 cgtccatggc ggcctgggtc ttggcgcggg gctcggccag cgagcgcacc cggccgcccg
    21361 gtgcggcgga gtccggtccg ccctgcgcct cgtagacgcc ggtcttctgg tagtagcgca
    21421 cgcgctggcc gcggatggtg tcgtagggct tgccgcagat gtagcggatc accacggcct
    21481 ggccgatcag gtcgaactgc tgtttggcgc cgtcgtattc ggcgcgcagg cccttgccct
    21541 cgatgcgttc gaaggtttcg gggcggtctt cggtgagcgt cacgaccttg ctcttgtcgg
    21601 cggtggccgt gccgaactgg ttgccgtcct tgtcttcgtt gacgacgagc ttgtcggagt
    21661 agatgttgag ctggccgcgc gtggccacca cattgccggt gaaaatgctt tcgcgcttgt
    21721 tgtcgtcgta gtgcagcgtg tcggagacga cctgggtgct gggttcgtcg gcgggggcgg
    21781 tcttggcctg gccttgcgcc ggggcctgcg cctggatttg ggcctgggcg gatgcggcgg
    21841 ccgtggccag cagcagggcg cccaggaggc gcggcaggat gggtgcggtc tggtgggtca
    21901 tggcttgttg gtcgggttcg tggccttgcg ggtttcgttg ggggcgaggt tgacgtcgct
    21961 ggacgagtac acctgcagct gccgggtctt gttgtcgtag cgcatgccga cgccgttcat
    22021 ggtcgacttg cccttggtca cgcgggcggg caggtcggta tagaccacgt cgtcgtcggg
    22081 catgatgatg aactgctcgc tgtaggcatc caggggatcg ttgttggcat cgggctggcg
    22141 gtgcacgtgc gcgtcgccga tcatggtgat gcgcttgctg ttgtggtcca tgatggcggt
    22201 cttggcggtt ccgacggtga tggggttgcc ttcctgctgg cccaccgcgc gcggcgtggt
    22261 gacgtggtag gagtcgtcgt cggggaaatg ctcgccgtag acgccttcga gccggttgat
    22321 gggcttgccg gcgggatcgg tgcgcagcat gacgaagttg cgcgtccagg cgtccatctc
    22381 gtgcgtcagg cgccgcggcg ggtcggtcag gatggcgcgc tgcgcgtaat ccgcggccca
    22441 ccaggtgccc atgaccaggg caaccagcag caccagcgcc atcagggcgg ggaaacggtc
    22501 tttcatgtcg gctactggat ggcgccggcg ccgaacagcg gctgcgtcgt caggaagctg
    22561 cccaggcggc cgcgcgcggc cagcagcagg tcgcagcatt cgcgcacggc gccgctgccg
    22621 ccggggcggg aggacaccca gtgcgcggac tgcgcgacgt agtcgagcgc attgggcacg
    22681 ctggcggcaa agccggcgcg ctgcatggcg ggcaggtcga tgatgtcgtc gcccatgaag
    22741 ccggtttcgt tgaccgaaat gccgtgtcgc tgcgtgagct cggtcagggc cgcgcccttg
    22801 tcgcgcacgc ccaccagcac gtcggcgatg ccgagctcgg cggcgcgccg gccgacgatg
    22861 gagcccgaac ggcccgtgac cagcgcgact ttcacgccgc tttgcatcag cagcttcagg
    22921 ccgtggccgt ccagcgcgtg gaagcgcttg aacgactcgc cgttttcgcc gtagtagagg
    22981 ctgccgtcgg tgagcacgcc gtcgacatcg aacagcatca ggcgcacggc ggccgcgagt
    23041 tcgcgcacgg cgggcgccac gcgggccagg accagcgctt cgacggggtg ggcaagcttg
    23101 ggggaattca tgggttgtcg ggccgccgtt gtcagaccac tttcgcggcc atgaggtcgt
    23161 ggatgtgcag ggcgcccagg agcaggccct gcgcgtcgac gaccagcatc tggttgagcc
    23221 gctggtcgtc catcagctgc gcggcttcgg cggccagcgc gtcggggccg atggcgtgcg
    23281 gcttgcggga catgccggcg gccacggtaa ggccgcgcac gtcgccctgg cgttcgatca
    23341 ggcggcgcag gtcgccgtcg gtgaagatgc ccaccggatg gttggcgctg tcgaccacgg
    23401 ccgtcatgcc catgcgcttg gcggagattt cttccagcgc gcgaaacagc ggcgcgtctt
    23461 ccggcacggt gggcaggtcg gggccgtggc gcatgacgtc gcgcacgtgg gtcagcaggc
    23521 gccggcccag cgcgccgccg ggatgcgagc gggcgaagtc ttccttgccg aagccgcggg
    23581 cttccatgca ggccacggcc agggcatcgc ccagcgccag cgaggcggtg gtgctggccg
    23641 tgggggccag gctcagcggg caggcttcct gggcgacgct ggcgtccagg tggacgtcgg
    23701 ccaactgggc cagttcggac ttggcgttgc cggtgatggc gatcagtcgc gcgcccatgc
    23761 ggcgcaccac cggaacgatg atgagcagct cggcgcccgt gcccgaatag gacagggcga
    23821 tcatcacgtc gtcggcggtg accatgccca ggtcgccgtg cacggcctcg gccgcatgca
    23881 cgaagaaggc cggcgtgccg gtggaggcca gggtggcggc gatcttgcgg gcgatgtggc
    23941 cggtcttgcc gacgccgctc acgaccacgc ggccgcggca ttccagcagc aggcgcacgg
    24001 cctggccgaa actggcgtcg aggcgggtgg agaggtcctg cagcgcctgc gtctcggtct
    24061 gcagcgtgcg gcgggccgag gccagggctt cgtcaggaga aaattgtgcg gtcatccggg
    24121 gattttaatc gcttaggctc ggcgccgggc cgttccaggc cgggccgtgc cccgcggagg
    24181 gcggtcgcgc cggcggccgg gggacagtca tagtaagccc ggcgccgggc gcggttgctc
    24241 gcgctggggg gctatggcat gctggcatgg ctttttttac ccatttcatc cgatttccat
    24301 ccccgatttc cattgctcat gaccgctttc cctgctcccg tgcttggcac ccccctgtcc
    24361 ccttgcgcca cccgcgtcat gctgctgggt tcgggcgagc tgggcaaaga ggtgatcatc
    24421 gcgctgcagc gcctgggggt ggaggtgatc gcggtcgacc gctatgccga cgccccgggg
    24481 caccaggtgg cgcatcgcgc ccacgtggtg tccatgaccg acgcccgggc cctgcggcag
    24541 gtcatcgagg ccgagcagcc gcacatcatc gtgccggaga tcgaggccat tgccaccgac
    24601 ctgctggtcg agctcgaggc gcagggcgtg gcgcgcgtca cgcccacggc ccgggccgcc
    24661 cagctcacca tgaaccgcga gggcatccgc cgcctggcgg ccgagaccct gggcctgccc
    24721 acgtccccct accagttcgt cgataccgaa gacgcgttgc gcgcggccat cgccgcaagc
    24781 atcggttttc cctgcgtgat caagccggtc atgtcctcgt cgggcaaggg ccagtcggtg
    24841 atccgcgacg aggccggcat cgccgcggcc tggcgccatg cccaggaggg cggccgggtg
    24901 ggcggcgggc gcgccatcgt cgagggcttc atccatttcg aatacgagat cacgctgctg
    24961 acggtgcgcg cccgcggcgc cggcggcgag atcgaaaccc gcttctgcga acccatcggc
    25021 cacaagcagg tcgacggcga ctatgtcgaa agctggcagc cgcaccccat ggcgccggcc
    25081 gcgctggccc gcgcgcgcga catcgccctg gccgtcacgt ctcaactggg cgggctgggc
    25141 attttcggcg tggaactctt cgtggccggc gaccaggtgt ggttctccga ggtcagtccg
    25201 cgtccgcacg acacgggcat ggtcaccatg atcacccagg cccagaacga attcgaactg
    25261 catgcgcgcg ccctgctggg cctgccggtg gacaccgcgc tgcgccagcc gggcgcatcc
    25321 agcgtgatct acggcggcgt cgaggcgcgc ggcgtgcgtt tttacaacgt ggcccaggcc
    25381 ctggccgagc cgggcaccga catccgcctc ttcggcaagc cggagtcctt cgtcaagcgg
    25441 cgcatgggcg tgggcctggc gacggcgctg gacgtgaccc aggcgcgcgc caaggcgcgc
    25501 cgtgtctctt cggcgatacg gatctcggcc gaagcttgat ctagatcaac caaacagtcc
    25561 ggggcggggc atccgccccg gatttctgtt gcgcctgaat aacaacatta ttaatatggt
    25621 tttgggctgg atttgctttt tgtttctttg agttcgataa tttcagtaat tattgaaatc
    25681 aaagtaacaa gaagaaaaaa tgccgctttc accgcagacc gagcgtttca tactgcactt
    25741 tggcgagatg ggcagccgct ggggcgtcaa ccgcaccgtg ggccagatct acgcgctgtt
    25801 gttcctggcg ccgcagcccc tgtgcgccga cgacatcgcc gagtcgctgg ccgtgtcgcg
    25861 ctccaacgtc agcatgagcc tgaaggagct gcagtcgtgg cggctggtca aggtggcgcg
    25921 tcgcgtggga gaccggcgcg actatttcga aacgccccag gatgtctggg agatattccg
    25981 tgtcctggcc gaggagaagc gcaagcgcga aatcgatccg accatgacgc tgctgcgcga
    26041 cacgctgctg gagccgcccg cgggcgagtc cgaggtccac gcccagcagc gcatgcggga
    26101 aatgctggaa ctcatcgagc tgtctaccgg ctggttcgat gaggtgcaac gcctgcctcc
    26161 cgagaccctg cgcaatttga tgaagctggg ctcgcgggta cagaaagtgc tggagttcgc
    26221 cggtaggctg cgcggccggg agtaggcctt gcgtcgccat tccaccgtaa gttcgaggag
    26281 gctgtcatga tcgatctgga tgtcgtcacc ctgtcccggc tgcagttcgc cgcgaccgcg
    26341 ctatatcact ttctgttcgt gccgctcacg ctgggcctgt ccttcctgct ggccgtgatg
    26401 gaaagcgtgt acgtcatgac gggcaagccc atctggaagc gcatgacgat gttctgggga
    26461 acgctcttcg gcatcaactt cgccctgggc gtgggaaccg gcgtggtcat ggagttccag
    26521 ttcggcatga actggtccta ctacagccac tacgtgggcg acatcttcgg cgcgccgctg
    26581 gcgctcgagg gactctcggc cttcttcctc gaggccacct tcgtcggcct gttcttcttc
    26641 ggctggaacc gcatgggcaa ggtcgcccac ctggcggtga cctggctggt ggcgttcggc
    26701 accaatctgt cggcgctgtg gatcctgatc gccaacggct ggatgcagaa ccccgtcggc
    26761 gcggtgttca accccgacac catgcgcatg gaaatggtgg atttcgccgc cgtgctgttc
    26821 aacccggtgg cgcaggccaa gttcgtgcac accgtcagcg cgggctatgt gctgggttcg
    26881 atgttcgtca tgtccatcag cgcctggttc gtgctcaagg gccggcacct cgacctggcc
    26941 aggcgctcca tggcggtggc ggccagcttc ggcctggcgg cctcgctgtc ggtggtggtg
    27001 ctgggcgacg agagcggcta tctcaccggc gagcaccaga agatgaagat cgcggccatg
    27061 gagtccatgt gggacaccga gtccgcgccg gccagcttca acctgctggc ctggcccaac
    27121 cgggccgagc acaagaacga cttcgcgatc cgcgtgccct acgtgatggg cctgatcggc
    27181 acgcgttcgc tcaccgagcc gctgctgggc atcaacgacc tggtcaagcg cgccgaaggg
    27241 cgcatccgct cgggcatcgt ggcctacgat gcgttgcaga agatccgcgc caaccccaag
    27301 gatgcggatg cccgcgaggt gttctcgcgc atctggcccg acctgggcta cggcctcctg
    27361 gtcaagcgct accagaccga tctttccaag gccaccgacg cgcagatcac ccaggcggcc
    27421 tgggacaccg tgcccacggt gatgccgctc ttcgtcatgt tccgcgtgat ggtgctggtg
    27481 ggcttctacc tgatcctgtt cttcgccctg atgttctgga tggtgtccaa ggacaggctg
    27541 gtgcactgcc cgcgcacgct caagctggcg ctgtggagcc tgccgctgcc ctggatcgcc
    27601 atcgagtcgg gctggttcgt ggccgaatac ggccgccagc cctgggtgat cgagggcgtg
    27661 ctgcccacgt actacgcggc atcgggcctg tccatgctcg acctggccat caccctggcg
    27721 gttttcgtgc tgctctacac ggtgctgctc atcgtcggca tcaaggtcat gctgcatgcc
    27781 atcaaggccg gtcccaaaga cagcccgccg gcgtttgccg gcggcggcgc gggttccgca
    27841 gcgggttcgg tacaagcggc gctggacgcc tgaacgcggg agaacgacga tggaaaacct
    27901 gattcctctg gactatgcca ccctgcgcgt catctggtgg gtgctgctgg gcgcgctgct
    27961 gatcggcttc gcggtcatgg acggcttcga tgccggcgtg gcggcgctgc tgccgctggt
    28021 gggcaagacc gatgccgagc ggcgtgtcgt catcaatgtg gtcggccccg tgtgggaagg
    28081 caaccaggtg tggctgatca ccgcgggcgg cgccatcttc gccgcctggc ccatgctgta
    28141 cgcggcctcg ttttcgggct tctacctggc catgatgctg atcctgctgg cgctgatcct
    28201 gcgcccggtg ggcttcaagt accgcagcaa gttcgaaggg acggcctggc gcaatacctg
    28261 ggacggcctg ctgtgctttt cgggcgtggt gatcgcgctg gtgtgcggcg tggccatggg
    28321 caatgtgatc ctgggcgtgc cgcacggctt cgaccccgac accatgcggc ccgtctacac
    28381 cggcatgttc taccagctct tcacgccctt cgcgctgttg tcgggcgtgc tgagcgtgac
    28441 catgctggcc atgcacggcg cggtgatgct ggcctggcgc accgaagacc ccatctccag
    28501 ccgcgcgcgc ggctgggccc ggctggcggc gctgctcacc atcgtgctct tcgtgctggg
    28561 cggtttctgg gtggcctatg gcgtggacgg gcaggtggcg ggccagatcg acatgggcgc
    28621 gccgtccaat cccctgatca agaccgtggc ggtgcaggcc ggcgcgtgga tggccaactt
    28681 ccacaagtgg ccgctgatgt ggatcgcgcc catcctgggc gtggcgggcg ccctgctggt
    28741 gctgttgatg cccggcgccg gcgcgctggc cttcatcgcc tcggcggcga cgatcgccgg
    28801 cgtcatcctc acggtgggct tcgcgctgtt tcccttcctg ctgccctcgt cgtcgcagcc
    28861 ggcggccggc ctgaccatct gggacggctc gtccagccac ctgacgctgt ggatcatgct
    28921 ggtggccgtg gtgatcttcc tgcccatcat cacgatttac accgcctggg tgtatcgcgt
    28981 catgcgcggc aaggtcacgc atgaatccgt gggcgacacg cccaactcgt attgagccag
    29041 gagctctctg ccatgtggta cttctcgtgg atactcggcc tgggcctggc ctgcaccttc
    29101 ggcatcttga acgcaatgtg gttcgagttg cgcgaggcga agcgccaggg cccggccacg
    29161 actgatcagt gagcggcgtc tcgggcatcg agcacacgcc gcaaaccgcc tccaaggccc
    29221 agtcggcctg gctgtcgggc caggcgcggc tggcgcgcgc gccgcttgcg ctggcgggcg
    29281 ccgcgccgct tgccgcgggc gccttgctgg cgctgcaggc ctggctgctg gccggcgtgc
    29341 tggatgcggc gctggctcac ggcgcggatc gcgaccagct cctgcggccg ctcgtcgcca
    29401 tcgttgcatt gatcctgctg cgcgcggccc tggcctgggt cgccgagcgc gccggtgcgc
    29461 gggcggccga agccatcaag caacgggtca ggctggccct gttcgatcgc ctcttgcggg
    29521 ccggcccgca atggacgcgc cggcaggtct cgggcgagct ggccagcgcg ctggtcgagc
    29581 aggtcgaggc gctggacggg ttcttcgcca aatacctgcc tgccgtgggg gcggcggcgg
    29641 ttctgcccgt ggcgttctcg gtgctgctga tgccgcagag cgtggtggcg ggcttgctgc
    29701 ttttcatcac cgcgccgctg attccgctct tcatggccct ggtgggctgg ggagccgagg
    29761 cggccagccg ccgtcatgcg cggaccactg ccaggctgtc gggttttttc gccgacaggc
    29821 tgcgcggcct ggccacgctc aagctgtacg ggcgggccga cgcggcggcg gccgacgtga
    29881 tgcaagccag cgacgccttg cgcgtgcgca cgctggcggt gctgcgcgtg gcttttctgt
    29941 cgtcggccgt gctggagttc ttcgccgcac tgggcgtggc cggcgtggcc gtgtatatcg
    30001 gcttgcgcta cctgggcatg ctgggcacgc ccgacccggg cttcggtttg aaggcgggtt
    30061 ttttctgcct cttgatggcg cccgaggtct atgcgccctt gcgccagttc gccgcgcact
    30121 atcacgaccg cgccgccgcg cgggccgccg tggccgagat cgatcgcttg ttcgacggct
    30181 tgcccggcac gggggagctt gaatggacgg tcgcgccgcc ggcggccatc gatggcgcgt
    30241 ccccggcgct gggcctggct gtgcaagggc tgaccgtcga agcgcccggc cggcccgcag
    30301 ccgtgctgca cgaggtgtcg ctgcgcctgg ccgccggcga acacgccgtg ctgatgggcg
    30361 ccagcggcgc cggcaagacc accttgctcg agacggtgtg cggcctgcgg acggcgcatg
    30421 gcgagatcct gctggacggc aagcccctgc gggactggga tgagacggcc ttgcgcgcgg
    30481 gcgtcgccct gatcgggcag aagccctatc tgttccaggg cacgatcgcc gacaacatcc
    30541 gctttgccaa gccgggcgcc agcgatgccg aggtggccga ggctgcccgg cgggcttgcg
    30601 taggggagtt tgcccagtcc ctgccgcaag ggctggacac gccgctgggg ccgcgcggcg
    30661 tgggcctgtc gggcgggcag gcgcagcgcg tggccctggc gcggctgttc ctgcgcgagg
    30721 cgggcctggt tctgctggac gagcccacgg cgcatctgga cgcggccacg caggacaggg
    30781 tgctcgatgc cgtcttcgcg tacgcgcgcg ggcgcagcct gctgctggcc acgcactccc
    30841 acgaggtggc ccggcggttt gcgcgccggc tgcatgtggc cgacggcaag gtgcgggacg
    30901 catgagccgt cggccacgcg tttcgatcgt cgcgctctat cgacgccagc gcgcgggcct
    30961 gctgctggcc ttgctgctgg ccctgctggc ggtggcggcg ggcgtgggcc tgctcggcgt
    31021 atcgggctgg ttcctggcgg gcgcggccct ggccggcgcc ggctccgaat tcaatctgtt
    31081 cgcgccttcg gcgctggtgc gcggcctgtc cttcctgcgc atcggcacgc gctatgccga
    31141 gcgcctggcc ggccacggcg ccacgctgca tctgctggcg gatctgcgcg gcgcggtatt
    31201 cggcgcgctg gtgcgactga cgccgcggca gttggcgcgc tttcgcggcg gcgacctggt
    31261 ggcgcggctc accggcgacg tggatgccct ggataccgtg tttcttttcg tgctggcgcc
    31321 catcgccacg gcattgctgg gggggctggc gctggcaata gtggtcgcct gcagtctgcc
    31381 gctggcgggc ctggtcctgt tgctgtgcct gttgtcgacc tgcctgttgt tgccggtcgg
    31441 cctggcgctg gccgcgcggc gttccggcgc ggcggtccag caaggcgccg ccgacctgcg
    31501 cacggccgtg ctcgatgccg tcgagggcca tgccgacatt gcagccatgg gcgtgcaggc
    31561 cgccgtgctg cggcacgccg acggcctgtg ccggcggcac ggccgggcac ggcagtcgca
    31621 atcgcgcctg gccgcgcatg gccagtgggt cctgcagatg gccgccggcc tggcggtgct
    31681 ggccgtgttg tggacgggcc tgcccgccat cgcggcggga cgcctgtcgg ggccgtacct
    31741 ggccggcctg ttgctggccg tgctgggcat attcgaagtg gccgggccgg tgatgcgcgg
    31801 cgcttcgcgc ctgggcgccg cgcgcgccgc ggcccggcgc atcgccggcc tgctcgatgc
    31861 cgagcccgat ctgcgcgacc cggcgcggcc cctgcccatg ccggcatcgg gcagcctgac
    31921 gatgcaaggc gtgcgctacg cctatccgcc ggcgccgggc gcggacgcgg cgcccgcctg
    31981 ggcgctggac ggcatcgatc tcgcggtcat gcccggcgaa cgcgtggcga tcaccgggcc
    32041 cagcggctcg ggcaagtcca ccctgctgca cctgttgctg cgcctggacg atcccgcggc
    32101 gggcgaggtg cgctacggcg gatgcgacct gcgccagtgc gcgcaggccg acgtgcatcg
    32161 ccgcattgcg ctgctggcgc aggacgcgcc ggttttcctg ggcacggtgc gcagcaatct
    32221 gctgatcggc gatgcccagg ccgacgaggc cgcgctctgg tcggccttgc aggcggcgcg
    32281 gctcgacacc ttcgtgcgcg gcctgccgcg cggcctggac agctgggtgg gcgagacggg
    32341 cgccgcgctg tccgccggcc aggcgcggcg cctgtgcctg gcgcgcgccg tgcttgcgcc
    32401 ggccgcggtg ctcttgctgg acgaacccac ggccgggctg gacgccgcca ccgaagccga
    32461 tttcctgcgc gatctgccgc aggccctggc cgggcgcacg gtggtgctgg tcacgcacgc
    32521 ggcgctgccc catggtgcgg tgcatcgccg cctgcgcatg gccggcgggc gcctcgaagt
    32581 cttgccggaa actgtgtaag cggccgaccg gcgccgggcg gttgcggtta tagtgcggct
    32641 tgcttctttc ttcgcgggac gtccttcatg cagaccgatt acgccgacct catccggcgc
    32701 actatccgca gcgttcccga ctggcccgag gccggggttg tcttccgcga catcaccccg
    32761 gtgctgcagg atccccgcac gttccgggcg ctcatcgatc tgttcgtcta ccgctacatg
    32821 cgccagcgcg tcgacctcat cgcctgcgtc gatgcgcgcg gtttcatcat cggcagcgcg
    32881 ctggcctatg ccctgaacgt gggtttcgtg ccgatccgca agcatggcaa gctgccgtgc
    32941 cgcacggtgt cggagtccta cacgctcgaa tacggcacgg ccgaggtcga gatgcatgcc
    33001 gatgcggtgc ataccgggca gcgggtcgtg ctgatcgacg acctggtcgc caccggcggc
    33061 accatgctgg ccgccatcaa gctgctgcag cgcctgggcg ccaatgtgct cgaagcggcc
    33121 accatcgccg acctgcccga cctgggcggc tcggagaaga tcaaggccac gggcacgccg
    33181 ttctacgcca tctgcagctt cacgggcaag gactgaagac cttcgccggg caggccggtc
    33241 aggccagcat cgaccaggcc gagcgcccga tcagcagcag ggccagcagc gccaggatga
    33301 ggacgcagac gcggcgatag tgcgtgtcgc catggcgcag gaacaggcgt tgccccagca
    33361 ggatgccggc ggcttgcagc ggataggtgg ccgccatgta cagcagcgtg cgcatcgaca
    33421 cgctgcccag cgcggccagc gtcagcagcg acaccgtgct ctgcaccgcg aaaatgacgg
    33481 tcatggtggc gcgggcggcc agcgccgaga acggaccccc caggaaatac gcgatcaggg
    33541 gcggcccgcc catggcggca aagccattgc ccaggcccga acaggcgccg accgagagcg
    33601 tgagtcccag cctgggctgg ccgctgtagc gccaacccga tgccaggaac acgacggtcg
    33661 ccagcacgat gcagcccatc accagccggc tggtggacgg gtcgaccgcg accagcagcc
    33721 gggcgccggc cagggccgcc aggctgctga ccagggacag gatgatggct tgccgccaca
    33781 ggatgtgcgg cctgtcgtgg cgcacggtct gcaggctgga caggatttgc aggcccgtca
    33841 tgatgggcgc cgccagcgcg ggcgcaaaca ccatggacag cgccggcatg ccgaacagcg
    33901 ccgcgccgaa gccggaaaag ccgcgcatcg tgcccgaggc gaggatggcc aggccggcga
    33961 aggccagcgt ccagggatgg gccgtcagcg atgcggcggg cgcgatgtcg tccaaccggg
    34021 gctgcgggtc aggccttgtc gcgatcgacg gcgaacgccg accaggcctg gcgggtgggc
    34081 atgatctcga gccggttgat gttgatgtgg tcgggcaggg tggccacgta gaagatctgt
    34141 tcggcgatgt cttcggccgt gagcggcgtg gtgctgcgat agagcttgtc ggaggccgat
    34201 tggtcgccgc gggtgcgcac cagcgtgaat tcggtctcgg ccatgccggg cgcgatgtcg
    34261 gtcacgcgca cgccggtgct cagcaggtcg cagcgcaggt tgtagctgaa ctgctgcacg
    34321 aaggccttgg tggcgccgta gacgtggccg ccggggtagg gccactggcc ggccaccgag
    34381 cccaggttga cgatgctggc gccggcgccg gtctcgagca gcatgggcag cacggcgtgg
    34441 gtcacgttga ccaggccggt gacgttggtg tcgatcatgg tgtgccagtc ctgcagttcg
    34501 acgttctgcg agggcacggg cgccagggcc aggccggcgt tgttgatcag cgagcgcacc
    34561 gggcggaatt cggcgggcag ggcatcgatg gcggcctgca cggcgcgggc atcgcgcacg
    34621 tccagggtgg cgatgtgcac cggcaccttg ggcgagagtt ctttttgcaa ttcttccagg
    34681 cgatcggtgc ggcggccggt gagcaccagc gaccagccgc cggcggcgaa gcggcgcgcc
    34741 gtggcgcggc caaaaccgga cgtggcgccg gtgatgaaga cgactttttt catgatcagg
    34801 tttcctgcgt gggcgaatgt tcaatattac aaaaacaact tgtacgccgg attctccgtc
    34861 tcgttccagt gcggataacc cagttcggcg atgaaattct tgaactgctt cttctcgccg
    34921 ggcggcacct ggatgccggt caggatgcgg ccatagtcgg cgccctggtt gcgatagtgg
    34981 aacaggctga tgttccagcc tggattcatg gcgttcaaaa agcgcatcag cgcgccgggc
    35041 cgctcgggaa attcgaagcg gtaaagcacc tcgtgggccg cttgcgacga atgtccgccc
    35101 accatgtgcc gcaggtgggt cttggcgaat tcgtcgtggg tcaggtccag cgtgtcgaag
    35161 ccgttcttgc ggaagtgcgc ggcgatcttg cccggctcgg cgggcgcgga cacctgcagt
    35221 cccacgaaca catgggcgcg gtcgccgtcg gacatgcggt agttgaactc ggtgacgctg
    35281 cgctcgccca ccagctcgca gaaacgccgg aagctgccgc gctgctcggg catggtcacg
    35341 gcgaacacgg cctcgcgcat ctcgcccacc tcggcgcggt cggagacgaa gcgcaggcgg
    35401 tcgaagttca tgttggcgcc gcaggcgatg gccaccaggg tcttgccgcg cagcttgtgc
    35461 tcggcggcat attgcttggc gccggccacg gccagggcgc cggcgggctc gagcacgctg
    35521 cgggtgtcct ggaacacgtc cttgatggcc gcgcagatgg cgtcggtgtc gaccaccacg
    35581 aaatcgtcca cgtattggcg cgtcaggcgg aaggtctcgg cgcccaccag cttgacggcc
    35641 gtgccgtcgg agaacaggcc cacgtcggcg agctgcacgc ggcggccggc gcgcacgctg
    35701 cgcagcatgg cgtccgagtc ctcggtctgc acgccgatga tcttgatgtc ggggcgcagc
    35761 tgcttcacat aggcggccac gccggcgatc agcccgccgc cgcccacggc cacgaagatg
    35821 gcgtcgatat tgcccgggtg ctggcgcagg atctccatgc cgatggtgcc ctggcccgcg
    35881 atcacgtcgg gatcgtcgaa ggggtgcacg aaggtgagct tctcgctttt ctcgagcagc
    35941 agcgcgtgct cgtaggcgtc ggtgaagctc tcgccggcca gcaccacttc gccgcccagg
    36001 cgcttgaccg cgtcgatctt gacctgcggg ctggtggtgg gcatgacgat gacggcgcgg
    36061 cagcccaggc gcttggccga cagggccacg ccctgggcat ggttgccggc cgaggcggcg
    36121 atcacgccgc ggttgcgaga ggccggcgcc aggttggcca tcttgttgta ggccccgcgc
    36181 agcttgaagc tgaacaccgc ctgcgtatcc tcgcgcttga gcagcaccgt gttcgaaatt
    36241 cgctgcgaga gcagggcggc gggttcgagc ggcgattcga cggcgacgtc gtagaccttg
    36301 gaggtcagga tgcgtttgag atagtcgatg gacatggagg acaggcgtgg gggtaggccg
    36361 gacgcccgct cggcggcggg cgcagcgggc tgacgagcgt accacacccc atgcatcgca
    36421 acccctggcg gcaacgttcc ccggctacac tgccccgcat catggtacat agtctgctcg
    36481 tcttcgtgca caagctgctg ggcctgctgg cgctgcctaa agtggggctg cccgccattt
    36541 tcctggtcag cctgatatcg tccagcgtgc tgcccgtggc gaccgagccc gtggtgttcg
    36601 gctacatcaa gctgtcgccc ggcatgttct ggccgggcgt gctggcggcc acggcgggca
    36661 gcatcctggg cggcatcatt ctctacttcg cgggccgcgg cgcgcaccgg gccgtgcagg
    36721 gcctgcgcca gcaccgcggg ccgtccggcg tgtcggacgg cggcgagatg ccggacctgg
    36781 ccgggcgcac gatcgcccgc catctcaagg ggcgtctggc catccgcgcc caggcctggg
    36841 cgcaccggct gggaccgtcg gcgctgctgc tgtgctggct gccggggctg ggcgacgcga
    36901 tgtgcctggt ggcgggctgg ctgaggttgc ccttttggcc cagcctggcc tgcatggccc
    36961 tggccaagct gggccgcacg ctcgccgtga ccgtcttcct gatgtgggcg gccgcgcatt
    37021 ggccgcagtg gcatctctag cgtctggaag acgctaggcc gcgcgcttca ggtagttctc
    37081 gcccaggatt ccccggacct cttcctggtt gaaccccacg ttttccagtg cttgcaccag
    37141 cgctcccaac tgctccggtt gcaggaagtc ccagggcggc ggcggatagc cgcgcggcca
    37201 gcggtccgcg gcggcatgga agaagccgta gtcactgcct tccaggtaca tgaagtccag
    37261 gcccaggccc acgtgcgcgg cgccgatcag gctggcgatg tgggcggcat gccgcgccat
    37321 ggcttgggga atgccggcgc cgcgcgcgcc gagaaacatg ccgacgccgc tgatgccgat
    37381 atagctgtcg cgatggccgc aggcgcggat ctgctcgtcc gtcagattgc gttcatggtc
    37441 gaagcaggcc cgcgcgttgg agtgcgagaa gaccggcacg cgcgccagcg gcgcgtccag
    37501 ggcctgcagg gaggtgagtg cgccgcagtg gctgaggtcg acccgcatgc ccgaggcgtc
    37561 catgcgcgcc agcaggcgat ggcccagcgt agtgaggccg gcgttgcggc tttcgtggca
    37621 gccgtccgcg tagggattgg cttcgttgta cgccaggatc gcgcggccga tgccggcggc
    37681 gcggaaggcg tcgacaaggt cgaggccggc gcagaacggg gtggcggtct ggaagtggaa
    37741 cgacaccgag aaccggccgc tcgcgcgcgc ctggcggatc tgcgcggcat cttcggcgac
    37801 ggcgagccaa tcccgcgccg gggccagtac ccgtcggaaa taaccgaggc gcgacagcgc
    37861 gcagggaaga tcgtcattgc ccgcggcggc ggtgagcgac acatggtcta tgccggcatc
    37921 gcgaaagcgc gccagctgtg ccagcaggtc cgcgccggac ggcaggaaag gcgccgtcca
    37981 cggcagcagg ccgtcgcaga tgggcaaccc cgccagccag gggcgtgcgt ccagccagcc
    38041 gggagaagcg gggcggttca tgtcgtcgtc tccaatgccg cgcggacttt gcttcccagg
    38101 ccggccagcc ggtgcgcggg ttcattggcg tagacgaagc ggatgtagtc ggcgccgtgc
    38161 tgtttccccc agcccgtcat ggcggtggcg catacgcctt gttccagcaa gcgctgggcc
    38221 agggtttcgc cggacatgcc gaaatcgctc gtgcgcagca gcagcgacca gccgcccgcg
    38281 ggcaggccga aaggcaggcc gtccagctcg ccggcgagca tgtcgcgccg gcgctgcaat
    38341 tcggcgacgt aggcgggcaa gtcgtcgaca gagcgttgca gcgcgatggc ggcggcttcc
    38401 tgggcaatgc cgaccggcac cacgacgttg gccagggaaa ccgcgacgat gtcgggaatc
    38461 agcgcgtcgg gggcgacgat ccagccgacc cgccagccga tcatgcgcag ttctttcgag
    38521 gccgctccca cgctgatgca gcgctcggcc atgccgggca gcccggccgg gtgcaggacg
    38581 gtccgcccgt cgaacagcag gcgttccatg gccgagtcga cgatcagcag cagatcgtgc
    38641 gccacgcaca gcgcggcgac ttcggcccag tcctgcgcgt ccaacgtagc gccggacggc
    38701 atcgaaggcg acatcaggag catcgctcgg gtcttgggcg tgatggatgc ccgcagggcc
    38761 tccctgtcca gcgtccaggg ctcgcctggc gcaaagcgca gcgggacttg gcgcgggatg
    38821 ccgcccgcca ggcggatgcg gttcagcaag ccggcatagg tcgggtccgt cacgatgacc
    38881 tcgtcgccga cctcgatggt ggccagcagc acattgagta tccccgacag gccgcccgcc
    38941 gaaatgacga cattgccggg acggtagtcg acgccggtca gcgtcgagac gtggcgcgcg
    39001 gcggtcgcgc gcaggtggtc ctgtccgaca aaaggcaggt acgagttggc ggcgtcctgg
    39061 tctatggcgg cccgcgtgcg ggccagcgct tcggcgcagg gcggcaggtc ggtgtccagg
    39121 ttttccagcc gcagccaatc ccgggcctcg ccgtcggcga ccgcgcccat gcggtcgacg
    39181 ccgataccgg cgatatagtc gaggcgggcg ggtcgatggt gtcgcatggc gggcctgctc
    39241 ctgtagaatt gtagataata gataatctat ttttatagtg aagccacatg tcttcgaatg
    39301 tcaatacgga tcgttccctg gccgggcagg tcgtccaggc cttggccgcg cgcatcatcg
    39361 ccggcgacat cgcgccgggg gcgtggctgc gccaggatga gctggcggag gaattcaaga
    39421 ccagccatgt gccggtccgg gaagcgttgc ggcgcctgga ggcgcagggg ctggtggtaa
    39481 tggagcctcg ccggggggtg cgggcggcgc cgctggacgc ggcctcgatc atcgaggtgg
    39541 cgcggatgcg ggcggcgctg gaacccctgg ccctgcgcca tgccttgccc cgattcccgc
    39601 agggccaact ggcccgtctg cgggaatgcc tgcgcgcgcc ggggcccgat ctggatatcg
    39661 ccgggctgga agccgcgaat cagcagttcc ataccttgat gaccgagtcc tgcggcatgc
    39721 cgcggctcct cgccaccctg accgatttgc accaggccag ctcgcggcat ctgttcgcgg
    39781 cctggaaatg gctggactgg cggccccgct cggatcgcga gcacgaagag atcctggcgg
    39841 ccctccaggc gggcgctgtc gatgacgctt gcgcccggct ggcggaccat atccttgcgg
    39901 cgggaacggc gttggcccaa gcactggcgc aaactggccg atagtccggc gccggcgccg
    39961 cgccgccggg tgcgtaaatg ccatatggat gcccggcaga caggcggggc attgccccgt
    40021 tgggctggaa atccctgtaa cgtctctcat ccggccgctt tcacgcggct taagccagct
    40081 ctctagggtt attaccaggg ctgtctgatt taatgactta aaatggtttt ttaagggcca
    40141 ctccgtcgct tatgaacgcc cctctcgcca gccaagtcct tgcatcggcg gtgccgccgg
    40201 ctcccggcgc gcgcctgcgc gaaatcccct ataactacac ctcgttttcc gatcgcgaga
    40261 tcgtctctct gctgctgggc gaggacgcct ggcaactgct gtgcgacctg cgcggcgagc
    40321 gccgcaccgg ccgctccgcg cgcatgctct acgaggtgct gggcgacatc tgggtggtca
    40381 accgcaaccc ctatctgcag gacgacctgc tcgacaatcc caagcgccgc aagctgctga
    40441 tcgaggccct caaccaccgc ttgcgcgagg tcgatctgcg ccgcacgccc gctggcgagg
    40501 ccaccgacgt gcagcgcgac aaccgggtgg tcgacctgct ggtgctggcg cgctccgccg
    40561 tgctcgactt cgagcgccgc ttcgacgaga ccgcgcagat gcgccagcag gtccagcgcg
    40621 tgctgggccg ctgcacggcg cgcgacaaca tcaagttcga cggcctgtcg cgcgtggcgc
    40681 acgtcaccga cgccaccgac tggcgcgtcg agtatccctt cgtggtgctc acgcccgaca
    40741 gcgaggaaga aatcgcgccg ctggtgcgcg cctgcatcga gctgggcctg accatcgtgc
    40801 cgcgcggcgg cggcaccggc tatacgggcg gcgccattcc gctgacctgg aagtcggccg
    40861 tcatcaacac cgagaaattc gagaccctgg gcccggtcga gcagcaggtg ctgcccggcc
    40921 tgaccgagcc cgtggccacg atcttcgccg gcgccggcgt ggtcaccaag cgcgtgaccg
    40981 aggcggccga acacgccggt ttcgtgttcg ccgtcgaccc cacctcggcc gaggcttcgt
    41041 gcgtgggcgg caacatcgcc atgaacgccg gcggcaagaa ggccgtgctg tggggcaccg
    41101 cgctcgacaa cctggcctgg tggcgcatgg tcgaccccga cggcaactgg ctggaagtca
    41161 cgcgcctggc ccacaacatg ggcaagatcc atgacgtgga cgtggcgcgc ttcgagctca
    41221 agtggttcga cggcaacggc cgcccgggcg aaaagctgct caagaccgaa gtgctcgaga
    41281 tcgccgggcg cgtgttccgc aaggaaggcc tgggcaagga cgtcaccgac aagttcctgg
    41341 ccggcctgcc cggcgtgcag aaggaaggct gcgacggcct gatcacctcg gcgcgctggg
    41401 tgctgcaccg catgcccaag cacatgcgca ccgtctgcat ggagttcttc ggccaggcgc
    41461 gcgacgccat cccgtcgatc gtcgaggtca agggctatct cgacggcgcg ggccgcgcga
    41521 gcggcgccat cctggccggg ctcgagcacc tggacgagcg ctacctgcgc gccgtgggct
    41581 acgccaccaa gagcaagcgc ggcacgctgc ccaagatggt gctgatcggc gacatcgtgg
    41641 gcgatgacga aaatgtggtg gccgcggccg ccagcgaggt ggtgcgcctg gccaacaccc
    41701 gccacggcga gggcttcgtg gccgtgagtc ccgaggctcg caagaaattc tgggccgacc
    41761 gctcgcgcac cgccgccatc tcgcgccaca ccaacgcctt caagatcaat gaagacgtgg
    41821 tgattccgct gccgcgcatg ggcgagtaca ccgactatat cgagcgcttc aacatcgagc
    41881 tgtccacgcg caacaagata cgcctgctcg atgcgctcga cgcctacctg gccgaaccgc
    41941 tgcccctggg caaggcggcc tcggccgacg acgacgccag cctgacgccc gaggcgctgg
    42001 acgagcgcgc cggccaggcc gtggcgctca tcgacgccgc gcgccgccgc tggcagtggc
    42061 tgctcgacaa tctcgacatg ccgctggcgc aggccctgcc cgagctcgac ggcctgggcc
    42121 tggcgcacct gcacgccgtc ctggccgaac gcctggcgcg gcagcccgat gcccgcgtct
    42181 tcgacgtgct gcaggaccgc accgtgcgcg tgtcgtggaa gaccgaggtg ctggccggcc
    42241 tggcgggcct gttcccgggc gacgcttgca agaagatcat cgacgacctg cgcgccgtcc
    42301 atgcgcgcgt gttgaagagc cgcgtcttcg tggcgctgca catgcacgcg ggcgacggca
    42361 acgtgcacac caacatcccc gtcaattccg acgactacga catgctgcgc gaggccaacg
    42421 aaacggtggc gcgcatcatg caggtggcgc gcgacctcga cggcgtgatc tcgggcgagc
    42481 acggcatcgg cctgaccaag tacgagtacc tcaccgaggc cgagctcctg cccttccagg
    42541 actacaagcg ccgggtcgat cccaacgggc atttcaacgc cggcaagctc atgccgggcg
    42601 ccgacctgcg ccacgcctgg acgcccagct tcagcctgat gggccacgaa tccatcatca
    42661 tgcagcaaag cgagatcggc gagatcgcgg cgtcggtgaa gaactgcctg cgctgcggca
    42721 agtgcaagcc ggtgtgcgcc acgcacgtgc cgcgcgccaa cctgctgtat tcgccgcgca
    42781 acaagatcct ggccacctcg ctgctggtcg aggccttcct gtacgaagag cagacccgcc
    42841 gcggcgtgag catcaagcac tgggaagaat tcgaggacgt ggccgaccac tgcacggtct
    42901 gccacaagtg cctcagcccc tgtccggtgg acatcgattt cggcgacgtg tcgatgaaca
    42961 tgcgcgcgct cctgcgccgc atgggccgca agtcgttcaa ccccggcacg gcggcggcca
    43021 tgttcttcct caacgcgaag gacccggcca ccatcaacgc cacccgccgc gccatggtgg
    43081 gcgtgggcta caaggtgcag cgcgccgcgc acgacttcct gagctcgttc acgcgcaagc
    43141 agacggcgca cccgccggcc acggtgggca agccgccgct gcgcgagcag gtggtgcact
    43201 tcgtcaacaa gcgcatgccg ggcggcctgc ccaagcagac cgcgcgcaag ctgctggaca
    43261 tcgaagacgc cgactacgtg cccatcatcc gcgatccgca agccaccacg gccgacacgg
    43321 aagcggtgtt ctactttccg ggctgcgggt cggagcgcct gttctcgcag gtggggctgg
    43381 ccacgcaggc catgctgtgg catgcgggcg tgcagacggt gctgccgccg ggctatctgt
    43441 gctgcggcta tccgcagcgc ggcaacggca tgaacgacaa ggccgagaag atcatcaccg
    43501 acaaccgggt gctgttcaac cgcgtggcca acacgctcaa ctacctcgac atcaagaccg
    43561 tggtggtgag ctgcggcacg tgctacgacc agctggcggg ctatgagttc gagaagatct
    43621 tcccgggctg ccgcttgatc gacatccacg agtacctgct cgagaagggc atcaagctcg
    43681 aaggcgtgac gggccagaag tacatgtatc acgacccctg ccacacgccc atgaagctgc
    43741 aggatccgat gaagacggtg aaggcgctgg tgggcgatgc cacgatcaag agcgaccggt
    43801 gctgcggcga gtcgggcacg ctggccgtga cgcggccgga catctcgacg caggtgcgct
    43861 ttcgcaagca ggaagagatg cagaaaggca tggaggcgct gggcgccggc ccaggcgacc
    43921 aggtcaaggt gctgacctcg tgcccgtcgt gcctgcaagg cttgtcgcgc tacgaggaag
    43981 acacgggcgc gaaggccgac tatatcgtgg tcgagatggc ccgccatata ttgggcgagt
    44041 cgtggatgga agactacgtg aagcgggcca acagcggcgg catcgagcgc gtgctggtgt
    44101 agcgcagcct ggcggctgcc tacaccatga gcatggtgga gagatccgcg gcaagcgcct
    44161 tcatggcctc ggcgacgggg ccttcgagct tgtcgcgggt catgacggag ctggctccgc
    44221 cgcaattcat ggccatgacg tggcggccat tgggcgacac gacggccacg cccagcgcgt
    44281 tgacgtcgag atggtagcgg ctcaggttca gcacgtagcc gtggcgaaga taggtctggc
    44341 gcgactcttc caggtattgg cggttggccg cccacagctg cggctgcgtt tgctcgagtt
    44401 cgcgcagcac cgcctcgcgt tcggtgtcgg acacggccgc caggtaggcg ctgccgacgg
    44461 cggagttgcc gatggacagc gccgagccga tatgaaaatt gagcttgagc atggtgggcg
    44521 ctgaggcgcg cgcaaggatg cgcatgtcga ggtgatcgcg cccggccagc gagaccgagg
    44581 cggagaagcg gttggcgatg tccgtgatgt gcggcagcgc gacttcggca atgccgctgt
    44641 gcgacagcgc ctcgcaaccc aatgccagca cgcccggact cacgcgcagc cgctcgggtg
    44701 cgtagccggg caccagatag ccggccttct gcagcgtgta gcacaagcgc cacgccgtcg
    44761 attgcgacag gcccagcagc cgggcgatgt cggtggagcc cagttccgtg cgctcggcgg
    44821 tgaaacagcg cagcacgtcc aggccgcgag ccagggcggt gacgaaatat cgatcttctt
    44881 tcatgggatg tccgagcggg ggtgacgcgc ggtgcgttga cgcgcgccgg atcacgtcgc
    44941 tacactaatt cgtaatgcaa ttttctaatt tgcagagtga attataagag ggagacaatc
    45001 tcatggcaaa cacatcggtg tccgccggcg tggacccgct ttccacgggc tttcccgatc
    45061 tttcccgctt tttcgcgccg cgtcgggtcg ccctgatcgg cgcgaccgaa gacctgtcca
    45121 aattcggcgg gcgctgcatg cgccagacgc tggatttcgg tttttccggc gcgctgtatg
    45181 ccgtcaatcc caagcgcgac agcattttcg gcctgccctg ccatgccgcc atcgcggacc
    45241 tgcccgagac gcccgaccat gtgggcatcg tgctgcccgc gcaagccgtg ccgcaggcgc
    45301 tggagcaatg cgcggcgcgc ggcgtgccct tcgcgaccgt gttttcgtcc ggcttcggcg
    45361 aactgggcac gcccgaagga caggccgcgc agcggcgcat cgccgagata gcgcgagccg
    45421 gcggcttgcg gctgatgggg ccgaattgca acggcatgat caacttcgtc gacggcttcg
    45481 cgctgacctc caccgccacc atacgcgggc cgcgccggcc agcaggcgac atcggcgtgg
    45541 tcagccagtc cggcggggcg gggcaggtca atgtcatgtg gcgcgcgcag cagtcgggcc
    45601 tgggcatcag ctaccaggtc agttgcggca atgcggccga tctggacctg accgactatg
    45661 cgtatttcat ggtggaaagc gaggcgaccc gcgtggtggt catgctggtc gagcgcctgg
    45721 ccgacggccc caggctgcgc cggctggccg aacgcgcggc cgaactgggc aagcccattg
    45781 tcatggtcaa ggtggggcgc accgaagccg gcagccgtgc ggccgcctcg cacaccggcg
    45841 ccatcacggg cgccgacgag gtttgcgatg ccgcgctgcg ccagctgggc atcatccggg
    45901 tggacgactg caacgaactc tacgaaaccg ccatgttgct gcgccagggc cggacggtgc
    45961 gcggtcgcgg cgcagccgct acctcgatat ccggaggcaa cctggtcatg gtggccgacc
    46021 tgggcgcctc ggtgggcatc cagtggcccg agtacgcgca gggcacgcga gacaagctgg
    46081 ccgagcaatt acccggattc ggctcggcgt ccaatcccac cgatctgacg gcttcggcca
    46141 tcggccagga aggcgcctac gagcgcgccg cgcgcaccat cctggccgac ccgtcggtgg
    46201 atgtgatgat tccggtgctg accatcgccg gcgccgccga tgtgaaggct gccgcgcgcg
    46261 tggcggccga cagcgacaag ctggtgccca tcctgtggac cggttgcgct gccgacgacc
    46321 ccgatctgac gcctgccgcg ctggtggccc agggcctggc ggtgtatcgc gatgccttgc
    46381 cctgcctgaa agccgtgcgc cgcgccatgg actacgccga actgcagcgc cgccgcggcg
    46441 agccggcgtc cgggcgtccc ggcggcgtcg acgcgcaggc cgcgcggcgc ctgctggagc
    46501 aggccgacgg tccgctcagc gagcaccggt ccaaggccct gctggcctgc tacggcattg
    46561 cggccccgca ggagtgcctg gccggctcgg tggcgcaggc cgtggatttc gcagcagcgc
    46621 tggacgcgcc ggtggtgctc aaggtgcagt ctcccgacat cgcgcacaag accgaggccg
    46681 gcgtcatccg gctgggcgtg cgcgggccgg ttgcggtgtc ggcggcctac ggcgaggtca
    46741 ttcaggccgc gcatgcttat cgtcccgacg cgcgcatcga aggcgtgctg gtgcaggaaa
    46801 tggtgaccca ggctcaggaa atgctggtgg gcgtgtcgcg ggacccgact ttcggaccgg
    46861 tcatcacgct ggcgctgggc ggcatttatg tcgaagtgat caaggacgcg gtgttccggc
    46921 tggctccgct gaccccgcag gaagcgcgcc gggcgttggg cgaactgcgc gctttccgcc
    46981 tattggaagg cgcgcgcggc caaccggccg ccgatatcga tgcgctggtc gatgccgtgg
    47041 tgcgcgtgtc ctggctggcg gccgacctgg gcgagcacat ctccgaactg gacatcaatc
    47101 cgctggccgt tctgccggcc gggcgcggcg tgcgcatggt cgatgcgctg gtcgtgccgc
    47161 ggcgcggttg acgcggaggt ccgccatgat cgagaccgcc atgcagccgc cggtgctgtg
    47221 gctgaccgtc tcccggcccc aggctgccaa tgccctggat gcggccacgc atgacgcgtt
    47281 ggccgccgcg ctgcgcgagg cggcgcggcg ccccggtgtg ggcgcggtgg tgctggccgc
    47341 cgcgggcggc aaggcttaca gcgccggcgc cgacctgcgt gaattttccg agtggccggc
    47401 cggccgcgcc gccctgcgcc gccgcgaatt gctgctgcag acgctggagg cgctgctggc
    47461 gttgggcaag cccgtggtgg ccgccgtgca ggcgccggcc atcggcgcgg gcgccatgct
    47521 ggccctggcc tgcgacgaga tcgtcatggc cgacggcgcc tggctgggtt ttccggaagc
    47581 ccagcacggc atgcctacgc ccatgggcgc ggtgctgctg gcgcgccgcg cgcgctggcc
    47641 ggtagtgcag cgcctgctgc aggcaggcga gcgttgcgcc gcgttcgagg cattgcaggc
    47701 cgggctggcc gacgactgcg tcgcggccgg cgtgctgccg gcacgctgcc aggcacgcgc
    47761 cgcagcgctg gccgccattc ctgcccatgc ctatgccgcc aacaaggcct ggctgaacag
    47821 gccgctcggc cacgcgctgg ccgaggccgc cgcgtatgcc accgcgcatc aggcaagcga
    47881 cccgtcccac tagaaaagag aggagacaag atgcagttgg aaatccaacc cgaactggcg
    47941 gaaatgcgcg ccgcggtgcg ccgcttcacc caggaaaaac tggaaccgat cgccttggcg
    48001 atcgaccata ccggcgaggt gccgcccggc acgctggaac tgttgcgcga acagggctac
    48061 ctgggcatgc gcatgcccgc ggccttcggc ggcggcgact tcgacctgcc cacgtattgc
    48121 ctggtgctgg aggaattgag ccgttctcat cgcatcttca ccttgatgct ggatgccacg
    48181 agcggcttga accccatcgc catcgcgcgc ttcggcaccc cggcgcaaca ggcccgctat
    48241 ctgtccggcc tgtgcgccgg caccttgcat gcgtccttcg ccttgaccga gcccgaagcc
    48301 ggcagcgacg cgcaggccat acgcacgcgc gcggcgcggg agaacggcgg ctggacgctg
    48361 aacggccgca agcatttcat cagtggcgcg catctggccg acgtggtgct ggtgatggcc
    48421 gttaccgacc cggccaagcg cggcgcgggc ggcattaccg ctttcctggt cgacaaggga
    48481 acgccgggct ttgccgtgac gcgcatcgac accacgatcg gttccgagcc gatcaagctg
    48541 gccgagctgt ccttcacgga ctgccgcgtg cccgacgagg cggtgctggg cgaggtggga
    48601 cagggcttca aggtggcgat ggcgtcgttg accagcggcc gcatgggcgt ggcctgctct
    48661 tgtatcggcg cggccgacag gctgttggaa atgagcgccg cctacgcgcg cgagcgccag
    48721 actttcggca agccgctggc cgagcgccag gccatccaat ggatgctggc cgactcggcc
    48781 accgaactgg ccatggcgcg cagcctcacc tacgaaaccc tgcgccgcct gctggctggc
    48841 gaaaacatcg gcgcggcggc cagcatgtgc aagctggccg cctcggaaat ggtgggcagg
    48901 gtggccgatc gcgcggtgca gatccacggc ggcatgggat tggtgcgggg ttttctggtc
    48961 gagcgtttct accgcgacgt gcgccattac cgcgttggcg agggcgcttc cgaggtgcag
    49021 cgcatgctga tcgcgcgcga ggtgttgcaa tgagcgcgcc cgccgcggcc atcgtcggca
    49081 taggcgaaag tccggtgggc cgcgtgcccg gtctcgacac gctgggcctg cagcgcgccg
    49141 ccgcgctgcg cgcgctggcc gatgccgggc tgtcgccggc cgatgtggac ggcctgctca
    49201 ccacgcccat ccgggtggag aactgggcca tgccctgcgg caatgtggcc gaggggctgg
    49261 gcctgcgccc gcattacctg gccacgctgg acgtggccgg cgcctccggc acggccatgg
    49321 tgcaccatgc ggccatggcc gtggcgacgg ggcaatgccg caccgtgctg tgcgtgggcg
    49381 ggcagaacct gctgtctttc agttcgagcg gcgcggcggt gcagaaactg gccgacgcgg
    49441 gctgggcgca tcccgagttc gaggcgccct acggcccgct ggttcccacg ctctacgcct
    49501 tggccgcgcg ccgccatatg cacgagtacg gcaccaccca ccgccagatg gccgaggtgg
    49561 cggtgaccat gcgccggcac gccgcgctca atcccaatgc gcacaaacgc gagccgctgt
    49621 cggtcgagga cgtgatggcc tcgcgcatga tcacctcgcc gctgcgcatg ctggattgcg
    49681 cgctggtatc cgacggcgcg gcggccttcg tggtgaccac gcccgagcgc gcgcgcgacc
    49741 tcggccagcc gccggtgcgc ctgctgggca gcggctatgg ccatagccac acctatatcg
    49801 gcgactatcg cgacgtgacg acgaccgggg cggtgcagtc cggccgcgat gccttcgcca
    49861 tggcggggct caagccggcc gacatcgacg tggcgcagct atacgactgc ttcaccatca
    49921 cggtcatcgt cgagctggag gacctgggct tttgcgccaa gg
//
LOCUS       DASWPO010000320         7025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_253972, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000320 DASWPO010000000
VERSION     DASWPO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253972"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VGC69_16830"
     CDS             <1..1164
                     /locus_tag="VGC69_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEY0296997.1"
                     /translation="DRAILDADVPFLEGPPLAASEHDAYYTPRVSQASAPLFEHCVRA
                     LEQSLDLFGAHGLPLMGTGDWNDGMNRVGEAGRGESVWLGWFLHATLSAFAPLARARG
                     DGQRADRWLLRAEALREALESAGWDGEWYRRGYFDDGTPLGSASNDECSIDAIVQSWS
                     VLSGAADAARAERAMAALDARLINRKDGLAVLFAPPFDHGALDPGYIKGYPPGIRENG
                     GQYTHASVWSMLAFAQMGDGDRAAYLLSLMNPINRSLTRSGAARYKVEPYVMAADVYS
                     VAPHVGRGGWTWYTGSAGWMYRAGIEGLLGLRKRGAELLIEPCIPKQWPGFTGVFRHG
                     AARYDITVENPRGVSRGVSRMVVDGVVLEPGKRAVPLADDGARHQVTVTMGEP"
     gene            1173..2483
                     /locus_tag="VGC69_16835"
     CDS             1173..2483
                     /locus_tag="VGC69_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY0296998.1"
                     /translation="MPDSSWYLLVGLLLITMVLVGATLARLLLSSATIYLCVGYFVGP
                     GGLKLVIPDPLLHAETIGQITEVALLISLFTVGLRVGVPIFNRRWIVPLRLAFVSMAI
                     TVGLIAAVGMWGLGLSLGAAILLGGILAPTDPVLASGMKTERGIDPDQLRFSLAGEGA
                     LNDGTAFPFVVLGLALLEPDNKAFSIWRWLMSDLIWGSVGGVLIGALAGFLIGRMVVY
                     LRTRHFQAVGQDEFLSLGVIAVTYGLAQLCHASGFLAVFAAGLAFQRIDETPQAGTVS
                     LGTTDGAQGHAYGVLATHSHHASAVMAGAVREFNAQLERLAVLTVVLLVGTLLPYATA
                     WRALWWFIPMMFVLVRPISVVLGTLGVAGAGRTRAMVSWFGIRGIGSVFYLMFAIHHG
                     VSKPLAGQLITLTLAMVSVSILVHGVSMRPLMKWYGRGQAPQEP"
     gene            complement(2505..2645)
                     /locus_tag="VGC69_16840"
     CDS             complement(2505..2645)
                     /locus_tag="VGC69_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0296999.1"
                     /translation="MAKGQLHGNREAKKPKQDKKPAPPTAGVPWTTPPKAAAVGKPDK
                     KK"
     gene            2919..4124
                     /gene="argE"
                     /locus_tag="VGC69_16845"
     CDS             2919..4124
                     /gene="argE"
                     /locus_tag="VGC69_16845"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY0297000.1"
                     /translation="MKNTARLPSDATVETLRTLIGFPTVSSVSNLDLIDYARAYLEGL
                     GCKTRMTGQASDGKYNLFATLGEGDPDTGLILSGHTDVVPASQGNWTSDPFTLRIAGG
                     RAYGRGTADMKGYIAVCMEQLRDLDPNTLKHPVHLALSFDEEVGCRGVPYLLDDLKAR
                     GIRPWGCVIGEPTSMSVVVGHKGSRRVCCTIHGKSAHSSQPLLGVNAIHFGSRLVGFV
                     DQLGTELRVNGPQDADYDVPYSTMLVTQIEGGEALNMVPQRCVLGFGLRHLPDHDPLQ
                     VIDEVRKFCEEQLLPDMQAWYPDAKIDFEVMGGVPSFNRSKDSGIIELVASELRALNA
                     GEATFGKVAFGTEAGHFEAHGIPSVVCGPGSISQAHQVDEYVDLSQLAEAETFISGLL
                     KRFCVEGKK"
     gene            4159..4893
                     /locus_tag="VGC69_16850"
     CDS             4159..4893
                     /locus_tag="VGC69_16850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0297001.1"
                     /translation="MKLENLTGKIAVVTGASSGIGEAAARLLAAEGMKVVLVARREDR
                     IRELAREIGEAAVAITADISDGVQVAAVFDQVRERFGALDLLFNNAGMGINGRFVDSK
                     PEDWKTQIDVNLYGVLHCTHAALPLMRGRVGAMVSTVSSVGGRYGIDGWSVYNATKFA
                     VIGLHDALRKELGGEGIRFSVIEPGSVWTEWGHNVPEDAMKKRRETVDALTAEDIANA
                     LVYSFAQPPRVLIEEMLIRPVKQIAP"
     gene            5066..6241
                     /locus_tag="VGC69_16855"
     CDS             5066..6241
                     /locus_tag="VGC69_16855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0297002.1"
                     /translation="MMTRRRWLTWGFVALGGVAVTLFAWLVFFSKPVPNPAPEPISVS
                     AATVSVQDVPLSITALGAAQAWTSATILAQVSGMLLSVDFVEGADVKAGQLLAQVDPE
                     PYRAVLTQAQGALERDRALLAGARVNLVRYQTLKAQDSIARQTFDDQAALVRQDEGTV
                     LLDEGVVATAQVNLGWCRIVSPIAGRAGVRTVDPGNLVTASASTSTTSAATGTTTSPV
                     GIVVINQIAPIAVTFSVPQGDYQRLSDASDAFRKPLPTQALSQETGALLGAGELRTAD
                     NRVNPATGTVEMKARFPNTDAHLLPGQFVNITLTLQTLSQVTTIPVAAVNQGPNGPYA
                     YVVGADQKVSMRTIKVGSTQGSTAVITSGLKSGETVVTDGQMSLDAGATVKVSSPAP"
     gene            6238..>7025
                     /locus_tag="VGC69_16860"
     CDS             6238..>7025
                     /locus_tag="VGC69_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0297003.1"
                     /translation="MSLSSPFIRRPVATALIAIAFLLVGIGGYVNLPVAALPNVDFPT
                     IQVSAQLPGASPETMASNVATPLERQFSLITGISQMTSVSSLGSTSITLQFELTQNIN
                     TDFEQIQAAITAASAQLPSNLPSAPTLRQVNPADAPVMILALTSDALPLALVDNYADV
                     ILSQQISRIEGVGLVTLGGQQKPAVRIRIDPRKVAALGLQIDKVRAQIVASTVNAPKG
                     AIKGPRQNLTIYANDQILDVAPWNQLVVGYHDGAAIRVKDLGDAV"
BASE COUNT         1168 a         2204 c         2334 g         1319 t
ORIGIN      
        1 gatcgggcga ttctcgacgc ggacgtgccg tttcttgaag gcccgccttt ggcggcgagc
       61 gagcacgatg cctactacac gccacgggta tcgcaggcca gcgcgccgct tttcgagcat
      121 tgcgtgcgcg cgctcgagca gagcctcgac ctgttcggcg cgcacggcct gccgctgatg
      181 ggcacgggcg attggaacga cggcatgaat cgggtcggcg aggcggggcg cggcgaaagc
      241 gtctggctgg gctggttcct gcacgccacg ctgtcggctt ttgcgccgct ggcgcgcgcg
      301 cgcggcgatg gccagcgggc agaccgctgg ctgctgcgcg ccgaagccct gcgcgaggcg
      361 cttgaaagcg cgggctggga cggcgagtgg tatcggcgag gttatttcga cgacggaacg
      421 ccgctcgggt cggccagcaa cgacgaatgc tcgatcgatg ccattgtcca gtcgtggagc
      481 gtgctatcgg gcgcggccga cgcggcgcgg gccgaacgtg cgatggcggc gctggatgcc
      541 cggttgatca accgcaagga cggcctcgcc gtgctgtttg ccccgccgtt cgatcatggc
      601 gcgctcgatc ccggctatat caagggctat cctcccggca taagggagaa cggcggccaa
      661 tacacgcatg cctcggtgtg gtcgatgctg gcattcgcgc agatgggcga tggcgaccgc
      721 gccgcctatc ttttgtcgtt gatgaacccg atcaatcgca gcctgacgcg cagcggggcg
      781 gcccgctaca aggtcgagcc gtatgtcatg gcggccgacg tctattcggt tgcgccgcac
      841 gtgggccgcg gcggatggac ctggtacacc ggttcggcgg ggtggatgta ccgcgccggg
      901 atcgagggct tgctcggcct gcgcaaacgc ggcgcggagt tgttgatcga accgtgcata
      961 ccgaagcaat ggcctggctt taccggcgtg ttccgccatg gggcggcccg atacgacatc
     1021 acggtggaaa atccgcgcgg cgtcagccgc ggcgtgagcc gtatggtcgt ggacggcgtc
     1081 gtgctggaac cgggcaagag ggcagtgccg ctggcggacg acggcgcgcg tcaccaggtg
     1141 acggtgacga tgggcgagcc atagagcaag ccatgccgga ttcgtcttgg tacttattgg
     1201 tgggcctgct gctcatcacc atggtgctgg tgggggcaac gctggcgcgc ctgctgctga
     1261 gcagcgcgac gatttatctg tgcgtcggct actttgtggg gcccggcggc ctgaagctgg
     1321 tcattcccga tccgctgctc catgccgaga cgatcggcca gataacggaa gtcgccctgt
     1381 tgatttcctt gtttaccgtc ggattgcgcg tgggtgttcc gatcttcaac cgcagatgga
     1441 tcgttccctt gcgcctcgcc tttgtctcga tggcgatcac ggtggggttg atcgcggcgg
     1501 tcggcatgtg gggattggga ttgtccctgg gggccgcgat actgcttggc ggcattctgg
     1561 cgcccacgga tcccgtgctg gcctccggca tgaagaccga gcgcggaatc gaccccgatc
     1621 aactgcgctt cagtctcgcg ggcgaaggcg ccttgaacga cggcaccgcg tttccgttcg
     1681 tggtcctggg gcttgcattg ctggagcccg ataacaaggc tttcagcatc tggcgctggc
     1741 tgatgtcgga cctgatatgg ggcagcgtcg gcggcgtgct catcggcgcg ctggcgggct
     1801 ttctgatcgg ccggatggtc gtctatctgc gcacgcggca tttccaggca gtgggccagg
     1861 atgagtttct atcgctgggc gtgatcgccg tcacttatgg cctggcgcag ctgtgccacg
     1921 catcggggtt tctggccgtc tttgccgctg gcctggcttt tcagcggata gacgagacgc
     1981 cgcaggccgg cacggtctcc cttgggacca ccgatggcgc gcaaggccat gcgtacgggg
     2041 tgctggcgac acactcccat catgccagcg ccgtcatggc cggcgcggtg cgggaattca
     2101 atgcccaatt ggagcgcctt gccgtgctga cggtggtgtt gctggtcggg acgctgcttc
     2161 cctatgccac ggcgtggcgg gcgctctggt ggttcatccc gatgatgttc gtgcttgtgc
     2221 gtcccatctc ggtcgtgttg ggcacgctcg gcgtagccgg cgcgggacgc accagagcca
     2281 tggtttcgtg gttcggaata cgcgggatcg gctctgtttt ttatttgatg tttgcgatac
     2341 accacggcgt gagcaagccc ctggcggggc agttgatcac gctgacgctc gcgatggttt
     2401 ccgtatccat cctcgtgcat ggcgtatcga tgcggccgtt aatgaaatgg tatggccgag
     2461 gccaggcgcc gcaagagccc tgagcccacg cggccggaca ccgattattt cttcttgtcg
     2521 ggcttgccaa ccgcggcggc tttcggcggt gttgtccagg ggacgcccgc cgtgggaggt
     2581 gccggctttt tatcctgctt gggtttcttc gcttcacggt tgccgtgcag ttgacccttt
     2641 gccatgatgg ttctccaata tattcagtgc aacttcagcc gcggagggcg accgccgtat
     2701 tgatcattct gagcgcattc aggcgcgttg tcgcggtcta tttccgaaga gtggccaacc
     2761 ccagccccat cccggctcat aaaggggtgc atgcatggtt ttccagcgta cagacggcat
     2821 gcctactacg gggaaccgag ggtagggcgc agtcgcgggc tgatgcataa tcgaaagaag
     2881 ccccttgcgg gtatcacgca tgcaaaggcc ccttccccat gaaaaataca gctcggcttc
     2941 cgtcggatgc cacggttgaa acactccgaa ccctcatcgg ttttcccact gtcagctcgg
     3001 tgtccaatct cgatctgatc gactacgcca gggcctacct ggaaggcctg ggctgcaaga
     3061 ccagaatgac cggccaggcc agcgacggca aatacaatct gttcgccact ctgggagagg
     3121 gcgaccccga tacgggtctg atcctctcgg gccataccga cgtggtgccg gcctcgcagg
     3181 gcaactggac gtcggatcct ttcaccctgc gcatcgccgg cggccgcgcc tatggccgcg
     3241 gcacggccga catgaagggt tacatcgcgg tctgcatgga gcaactgcgc gacctcgatc
     3301 ccaatacgct caagcatccc gtgcatctgg cgctgagctt cgacgaagaa gtgggctgcc
     3361 gcggcgtgcc ttacctgctg gacgacctca aggccagggg tatccgtcct tggggctgcg
     3421 tcatcggtga gcccaccagc atgtccgtgg tggtggggca caaggggtcc cggcgcgtgt
     3481 gctgcaccat ccatggcaag tcggcgcact cgtcgcagcc cttgctgggc gtcaatgcca
     3541 ttcacttcgg ttcgcggctg gtgggtttcg tcgaccagct gggtaccgag ctgcgcgtca
     3601 acggcccgca ggacgcggac tacgacgtgc cctacagcac catgctggtg acccagatcg
     3661 aaggcgggga ggcgctgaac atggtgccgc agcgttgcgt gctgggcttc ggcctgcgtc
     3721 acctgcccga tcacgacccc ctccaggtca tcgacgaggt ccgcaagttc tgcgaagagc
     3781 aactgctgcc cgacatgcag gcctggtatc ccgacgccaa gatcgatttc gaggtgatgg
     3841 gcggcgtgcc ctcgttcaac cgttccaagg actccggcat catcgaactg gtggcgtccg
     3901 aactgcgcgc gctcaatgcc ggggaagcca ccttcggcaa ggtggccttc ggcaccgagg
     3961 ccggccattt cgaggcgcac ggcattccca gcgtggtgtg cggtccgggc agcatttcgc
     4021 aggcgcacca ggtcgatgaa tacgtcgacc tgagccagct cgccgaggcc gaaaccttca
     4081 tttccggcct gctcaagcgc ttctgcgtcg agggcaaaaa ataacggctg atggtcgctc
     4141 tcaaattagg aggcaaacat gaaattggaa aatctgaccg gcaagattgc cgtcgtcacc
     4201 ggtgcgtcga gcggcatcgg cgaggcggcg gcccgcctcc tggcggccga gggcatgaag
     4261 gtggtgctcg ttgccaggcg cgaggatcgt atccgggagc ttgcccggga aatcggcgaa
     4321 gccgcggtgg ccatcacggc cgacataagc gacggagtcc aggttgcggc ggtgttcgat
     4381 caggtccggg aacggtttgg cgctctcgac ctgctgttta acaatgcggg catgggcatc
     4441 aacggtcgct ttgtcgattc gaagcccgag gactggaaga cccagatcga cgtcaatctt
     4501 tatggcgtgc tgcactgcac gcatgcggcc ttgccgctga tgcgcggccg ggttggtgcg
     4561 atggtatcga cggtctccag cgtcggcggc cgttacggta tcgatggatg gtcggtctac
     4621 aacgccacca agtttgccgt gatcggcctg cacgatgcgc tgcgcaagga attgggcggc
     4681 gagggcatcc gcttttccgt catcgagccg ggatcggtct ggactgaatg gggacacaac
     4741 gttcccgaag acgccatgaa aaagcggcgc gagacagtgg acgcgctgac ggccgaggac
     4801 atcgccaatg cgctggtcta ttccttcgcc caaccgccgc gggtgctgat tgaagagatg
     4861 ctgattcgcc cggtcaaaca gatcgctccc tgacgaagcc gtccgcgcgt cactgagtcc
     4921 gccagcgtac agacccgcca ggcggacggt cacaccatgc tggcaagagc caaccggctg
     4981 aaaagagaaa agccgatgcg gttcgaccgt caactcgcac tcatgccggc cggcgccagt
     5041 ttgcgcggca acgggagggc gggtcatgat gacgcgtcga cgatggctta cgtggggctt
     5101 cgtggcattg gggggggtgg ccgtcaccct gttcgcctgg ctcgttttct tcagcaagcc
     5161 cgttcccaat cccgcgcctg agccgatctc cgtttccgcc gccacggttt cggtccagga
     5221 cgtgccgctt tccattaccg ccctgggcgc ggcgcaggcc tggaccagcg ccaccattct
     5281 tgcccaggtc agcggcatgc tcctgagcgt cgactttgtc gagggcgccg atgtcaaggc
     5341 gggccagctc ctggcgcaag tcgatcccga gccctaccgg gcagtgctga cccaggcgca
     5401 gggcgcactc gaacgcgacc gcgccctgct ggccggggcg cgcgtcaacc tggtccgcta
     5461 ccagacgctg aaggcgcagg attcgatcgc ccgccaaacc ttcgacgacc aggccgcgct
     5521 ggtccggcag gacgagggca ccgtcctgct cgacgagggc gtcgtggcca ccgcccaggt
     5581 caatctgggc tggtgccgca tcgtttcgcc gatcgccggg cgcgccgggg ttcgcacggt
     5641 cgatccaggc aatctcgtga ccgcgagtgc atcgaccagc accacaagcg ccgcaaccgg
     5701 caccacgacc agtccggttg gcatcgtcgt catcaatcag atcgcgccga tcgcagtgac
     5761 gttctcggtg ccgcagggcg actatcagcg cctgtcggac gcttccgatg cctttcgcaa
     5821 gcccttgccg acgcaggcgc tcagccagga aacgggcgcg ttgctgggcg cgggggaact
     5881 gagaaccgcc gataaccggg tcaatcccgc gacgggcacc gtcgaaatga aggcgcgttt
     5941 tcccaatacc gacgcccacc tgttgccggg ccagttcgtc aacatcacgc tcacgctgca
     6001 gacgctctcg caggtcacga cgattcctgt cgcggctgtg aaccaggggc cgaacggccc
     6061 ctacgcctat gtggtcggcg ctgaccagaa agtgtcgatg cgcactatca aggtcggctc
     6121 gacccagggc tcgacggcag tcatcacgtc gggtctcaag tccggggaga ccgtggtgac
     6181 ggatgggcag atgtcgctcg acgcgggtgc cacggtcaag gtgagctcgc ccgcgccatg
     6241 agcctctcca gccccttcat ccggcgtccg gtcgcgaccg cgctgatcgc gatcgcattc
     6301 ctgctggtgg ggataggcgg ctatgtcaac ctgcccgtgg cggcattgcc gaacgtcgac
     6361 ttcccgacca ttcaggtcag cgcgcagctc ccaggggcaa gtcccgaaac catggcctcg
     6421 aacgtcgcca cgccgctcga gcgccagttt tcgctgatta ccggcatcag ccagatgacc
     6481 tcggtaagct cgctgggttc gaccagcatc acgctgcagt tcgagctgac gcagaacatc
     6541 aataccgact tcgagcagat ccaggcggcg atcacggcgg ccagcgcgca gttgccgagc
     6601 aatcttccga gcgcgccgac gctgcggcag gtcaatccgg ctgatgcgcc ggtcatgatc
     6661 ctcgcgctca cctccgacgc gctgccgctc gcgctggtcg acaactacgc cgacgtgatt
     6721 ctgtcgcagc agatttcaag aattgagggg gtgggcctgg tcaccctggg cgggcagcag
     6781 aagcctgccg tccgcatccg gatcgatccc cgcaaagtcg cggctctcgg cctgcagatc
     6841 gacaaggtcc gcgcccagat cgtcgccagc accgtcaatg cccccaaggg agcgatcaag
     6901 gggccccggc agaatctcac catctatgcc aatgaccaga tcctggatgt ggcgccgtgg
     6961 aatcagctcg tggtcggcta ccacgatggc gcggcgatcc gggtcaagga cctgggcgac
     7021 gcggt
//
LOCUS       DASWPO010000321        14498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2040159, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000321 DASWPO010000000
VERSION     DASWPO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040159"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VGC69_16865"
     CDS             complement(<1..1045)
                     /locus_tag="VGC69_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEY0297004.1"
                     /translation="MALTTRCPHCRTAFKVVADQLRVRNGLVRCGACGTVFDGRACLE
                     GEQAAPVPAYAASSQASSLVNNPPALRTPVPAEPARPAVEPPAVLRGRDRHAVEPDPA
                     PADDGSGYEDDDNVDDREIGGHEAGDHGIGHPAAGHPPPIRAYDPEDYPPQQDEPEFT
                     VGGAQAETSGDGIYGDVRTRFSSADAGRAPPAFLDQDRINTHRTRRRIWAWLCLLALL
                     ALAGQLAYVYRTPIALSAPALRPVLAGLCKPMGCTVGYARRIERIFIESSSLRPPQGA
                     ASQTDEDGRSRLILAAVLRNRYDKDQEWPAVTLSLTDMSDTVIARKIIRPEDYLPPGT
                     AGPMAAGTEVTISV"
     gene            complement(1060..1986)
                     /gene="prmA"
                     /locus_tag="VGC69_16870"
     CDS             complement(1060..1986)
                     /gene="prmA"
                     /locus_tag="VGC69_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0297005.1"
                     /translation="MRELVLHCREAQAEALSDALLEAGALSVSVEDADEGTDGERPLF
                     GEPGTEPDVQAWDRNRVVALLPDGADPAQLLEAALAESGLDAGLGQGWSLRDVPDADW
                     VRLTQAQFGPIKIAEKLWIVPSWHRDDVAAPAAPSFLSPDAIRIELDPGLAFGTGSHP
                     TTHLCLAWLESELPAGATLLDYGCGSGILAIAARKLGAGATLAVDIDPQAVQSTADNA
                     AVNRVELSAMLPDGLPEGAFQVVVANILSNPLKVLAPMLAGRVAPGGHLVLSGVLERQ
                     AEEVAAAYAPWLAMSVWRAREGWVCLHGQRRR"
     gene            complement(2069..3418)
                     /gene="accC"
                     /locus_tag="VGC69_16875"
     CDS             complement(2069..3418)
                     /gene="accC"
                     /locus_tag="VGC69_16875"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0297006.1"
                     /translation="MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADRQAKYVRL
                     ADESVCIGPAPSRESYLNMPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFVF
                     IGPRPETIRLMGDKVSAKRAMIEAGVPVVPGSEGELPDDPQEIVRIAREVGYPVIVKA
                     AGGGGGRGMRVVYTEAALVNAVNLTRSEAGAAFNNPAVYLEKFLENPRHVEIQVLADG
                     GRNAVWLGERDCSMQRRHQKVIEEAPAPGIARRLIERVGDRCADACRKMGYRGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTELITGVDIVQQQILIAAGEKFNLRQRDVVLKGHA
                     IECRINAEDPFRFTPSPGRITNWHVPGGPGVRIDSHAFNGYFVPPNYDSMIAKVITYG
                     DTREQAMARMRIALSEMVVEGIQTNIPLHRELMQDSRFMEGGTSIHYLEQKLSQRPS"
     gene            complement(3435..3869)
                     /gene="accB"
                     /locus_tag="VGC69_16880"
     CDS             complement(3435..3869)
                     /gene="accB"
                     /locus_tag="VGC69_16880"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY0297007.1"
                     /translation="MDLRKLKTLIDLVAESGIAELEITEGEGKVRIVKFSQAVQPMAY
                     PMPEPAAAPAAAPAAAPAAPVIQGHAVKAPMVGTFYRSPNPGASPFVEVGQTVKEGDA
                     LCIIEAMKLLNEIEADKSGVIKEILVENGDAVEYGQPLFVIG"
     gene            complement(3963..4397)
                     /gene="aroQ"
                     /locus_tag="VGC69_16885"
     CDS             complement(3963..4397)
                     /gene="aroQ"
                     /locus_tag="VGC69_16885"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY0297008.1"
                     /translation="MAQSVLVLHGPNLNLLGTREPGIYGSRTLAQIDAGLAALAAELG
                     VKLAAWQSNHEGALVDRIQAAASDGTDFIIINAGAYTHTSVAIRDALAGVAIPFIEVH
                     LSNLFKREPFRQHSYLSDIAVGLISGLGADGYGAALRYAAGR"
     gene            complement(4531..5064)
                     /locus_tag="VGC69_16890"
     CDS             complement(4531..5064)
                     /locus_tag="VGC69_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0297009.1"
                     /translation="MNRRHLFRSGARLAAALLAASAGRAAQAAVPAAEPPLADALAAL
                     RVRPMADLQGATRRLAEWQGRVMVLNFWATWCAPCVKEMPELDALQKQFPQAQFIGIG
                     VDTVDNIRKFLVKVPVSYPLLVMGAGAVDTLRGLGDTAGGLPFTLILAADGGINRSIL
                     GKIQPDDVHDTLTNLGG"
     gene            5140..6678
                     /gene="mpl"
                     /locus_tag="VGC69_16895"
     CDS             5140..6678
                     /gene="mpl"
                     /locus_tag="VGC69_16895"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY0297010.1"
                     /translation="MHLHILGICGTFMGGLALIARSAGHKVTGCDAGVYPPMSTQLAE
                     QGIDLIEGWDPSQMALKPDLYVIGNVVTRGNPLMEAILNAGAPYVSGPQWLGDHILPG
                     QHVLAVAGTHGKTTTSSFLAWILECAGRRPNFLIGGVAPDLQVSARYESPRSPLAAPP
                     GLGTARRPTDLLAATFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEYDHADIFPDL
                     AAIETQFHHLVRTIPSQGRIVAPARDEAIDRVLARGCWSETVRFGAGPAPGGHALSGG
                     CWEAGEPANDGAFSVRRDGQEQGTVHWTLTGEHNRSNALAALAAAEHAGVPAAEGIAA
                     LSRFGGVKRRMELRGTVRGIKVYDDFAHHPTAIATTLAGLRRQIERGLASAGPSPAGD
                     PPRGDGAQRQGGRILAVLEPRSNTMKLGAMAARLPGSLADADQVFIYGEREGRHALGW
                     NPAEVLAPLGGKAHSYEQLDELVAAVARTARPGDHIVVMSNGGFGGVHGKLLDALARN
                     RAET"
     gene            6675..7277
                     /locus_tag="VGC69_16900"
     CDS             6675..7277
                     /locus_tag="VGC69_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEY0297011.1"
                     /translation="MKNRILYLHGFRSSPASFKGRMMEQALAERGLSPIWAGPQLPAS
                     PRQAADLALHIAQTQLRDRAGPKGNPAKLTVVGSSLGGYYATWVAEQLGCKAVLLNPA
                     VHAVRDLTPAVGTHRMYHSDAPFVFRPEYLGELAALRARRITRPRRYFLIAATGDEVL
                     NWREMRERYAGCRQHIVQGSDHGLSNFADLLPEVLAFALE"
     gene            7285..9168
                     /locus_tag="VGC69_16905"
     CDS             7285..9168
                     /locus_tag="VGC69_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HEY0297012.1"
                     /translation="MYVFYEDDGSFKAAHTMSETDATLQIESESGKRSKIKRAAVLFS
                     FDAPAPAELMPAAAALAEDMDLQFLWECAPQEDFAAPTLAADYFGHAPSPVEQAALVL
                     RLHGAPAYFHRRGAGRYRPAPPDILAAALAAIEKKRLQAEQQQQWVDDMAAGRLPEPI
                     AQAAEALLIKPDKNSQAWKALDAACARLQKTPERLLLELGAWPHALALLKRRFLATHF
                     PRGTGFPDVEIPPIERELPLADAEIYSVDDVTTTEIDDALSVRVLPDGRVRAGVHIAA
                     PGLAVTKGSELDKLARARLSTVYMPGDKIPMQPDAVISAFSLDAGRAVPALSLYVTAD
                     PATGEIFDTETRLERIAVRENLRHNLLDGQVTEARLADPQSDLPYGHWLRPLWQLALA
                     LSAKREEVRGKPENNTRVEYSFYLDGNPDDPDTPVRLVPRQRNAPLDRMVAEYMILAN
                     NLWGGLLAQHGVPGIYRSQQAGRVRMSTQALPHEAIGVPQYAWSTSPLRRYVDLVNQW
                     QLIAAVDNGVGARLVAPFKPKDADLYAIIGAFDAQYAAWAEFQASMERYWCLRWLAQQ
                     GITRCTASVLRDDLVRLSNAPFVTRVGGMPELERGTVVEIDILGRDELALELDCRYIG
                     PVA"
     gene            9174..10016
                     /locus_tag="VGC69_16910"
     CDS             9174..10016
                     /locus_tag="VGC69_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0297013.1"
                     /translation="MARRDDYLLIGLAISLMVHAGALAWRFAPRPAHPAAPALEVVLV
                     NAHSDTAPARAQALAQQNLEGGGEARQGLARSPLPRTGESAETIVLQAMHKRQQELEQ
                     EQARLLMQLQSSRQAAAPCQPANPDETAADPGQDPEEQAGVIQNAQVAALSQRVQAYN
                     KEPRRAYVGPATQASRYAAYLDGWTRRVEAIGTQHYPDDARGHTYGSLRITVRVKRDG
                     SLAGFEIDQPSQYAVLNQAARRIVQMAAPFPPFPPDIARDTDELVITRTWHFVNDTLQ
                     ASER"
     gene            10102..10959
                     /gene="aroE"
                     /locus_tag="VGC69_16915"
     CDS             10102..10959
                     /gene="aroE"
                     /locus_tag="VGC69_16915"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0297014.1"
                     /translation="MTDAPLHYAVIGNPVAHSRSPRIHALFGQQAGIALRYDRLPAPL
                     DGFEATAQAFAEAGGRGLNVTVPFKRQAFDLARGHLSDRARLAGAVNTLWRQDEAWHG
                     CNTDGVGLARDLVRLGARLAGARVLLVGAGGAARGVLQPLAAAGCAHLRIVNRTPGRA
                     RELAQGWQAAWTGLPVEVSAGGLPDAAIAGGWDVVINATASSLQGAAPDLPAGLYADG
                     ALAYDMLYAARPTPFMRQAAGDGAAQCADGLGMLVGQAAESFFIWHGVRPDPAPVLDA
                     LRAELTAQG"
     gene            10965..11684
                     /gene="mtgA"
                     /locus_tag="VGC69_16920"
     CDS             10965..11684
                     /gene="mtgA"
                     /locus_tag="VGC69_16920"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY0297015.1"
                     /translation="MARRRGRRVRWLAMLVMALLGLCLLYQLWMLGQVLWYSRYNPGS
                     SAVMRQELARLRKTDPRIALQHAWVPYERINGTLKRAVVASEDANFLEHDGVEWDAMR
                     EAWEYNQRQEELGRRTRRGGSTITQQLAKNLLLSNSRNYLRKGEELLVTFMIEQVMSK
                     RRILELYLNIAEWGTGVFGAQAAARHYYQIDASQLDAYQAARLAAMLPNPRYYDQHRD
                     TRYLNSRTATIGARMRQADIP"
     gene            11778..12959
                     /gene="lptG"
                     /locus_tag="VGC69_16925"
     CDS             11778..12959
                     /gene="lptG"
                     /locus_tag="VGC69_16925"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY0297016.1"
                     /translation="MRTARRYLALEIYRSCAVVLLALLGLFTFFSLVDDLDGVGKKFT
                     LLAMLYMQALETPSRLYDLLPIGLLIGAILALASLAQRNELVILRVSGVSGMRLLGTL
                     WLITVPMMAGALLLSEYITPKAEIRSSEVSLMLRGQASGGRLASGYWFKEPTATGGTR
                     IINIKTLMGNGAVSDVMVYELTPTLELSLVAQAPSGTLMGGGDLVLRNVTETRLDPNA
                     SEALADARPPAQPPATVVTEPELHLSTTLNPQRLLARVLTPERMSILSLIDYIDYLHR
                     NQLQADRQIVAMWRKAVYPFTLLVMITIAAPISFMQTRRGGVGAKVFIGILLGVGFFM
                     LNQLALNVGMLSRWPPWLTALGPNVGALLLALAALSAMEYRTILSNLVAGHWPWRKAL
                     A"
     gene            12956..13741
                     /locus_tag="VGC69_16930"
     CDS             12956..13741
                     /locus_tag="VGC69_16930"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY0297017.1"
                     /translation="MNSSIWMIGDVQGCCQPLEQLLSHPDITADPQSQFWFAGDLVNR
                     GPQSLESLRRIMSLESRSVTVLGNHDLHLLAAAAGVRKPSKSDTLDEILNAPDAADLI
                     DWLRNRPLAHFEHDHLMVHAGVLAKWDVAKTLSLAHEVQEALRGPNWQKSLQKMYGNE
                     PVNWKDGHTGGKRLRVIINALTRIRLCTPQGHMDFNAKVAPGAWPDGLIPWFDGPKRA
                     TRGVTIVFGQKKREREREYKTIVEMVVVSQSFGLWISFVLVLL"
BASE COUNT         2451 a         4931 c         4765 g         2351 t
ORIGIN      
        1 gcaccgagat ggtcacctcg gtgccggcgg ccatggggcc cgcggtgccc ggcggcaggt
       61 agtcctcggg ccggatgatc ttgcgcgcga tgaccgtgtc ggacatatcg gtgaggctga
      121 gcgtcaccgc gggccattcc tggtctttat cgtagcggtt gcgcagcacg gcggcgagga
      181 tcaggcgcga gcggccgtct tcgtcggtct gcgaggcggc gccctgcggc gggcgcagcg
      241 acgaggactc gatgaagatg cgctcgatgc gccgcgcgta gccgaccgtg cagcccatgg
      301 gcttgcacag gcccgcgagc accggccgca gcgccggcgc cgagagggcg atgggcgtgc
      361 ggtacacata ggccagttgc ccggccagcg cgagcagcgc cagcaggcac agccaggccc
      421 agatgcggcg gcgcgtgcga tgggtattga tgcggtcctg gtcgaggaac gccggcggcg
      481 cgcggcccgc gtcggcgctg gagaagcggg tgcggacatc gccgtagatg ccgtcgccgg
      541 acgtttcggc ttgcgcgccg cccaccgtga actcgggctc gtcctgctgc ggcggatagt
      601 cctcgggatc gtaggcgcga atgggcggcg ggtggccggc cgcaggatgg ccgatgccat
      661 gatcgccggc ttcatggccg ccgatttcac gatcgtcgac gttgtcgtcg tcttcgtacc
      721 cggatccgtc gtcggccggc gcgggatccg gttcgaccgc atgccggtcc cggccgcgca
      781 gcacggcagg cggctcgacc gccggcctgg ccggctcggc cgggacgggc gtgcgcaggg
      841 ccggcgggtt gttcaccagc gaactggcct gcgaactcgc cgcgtaagcg ggaacgggag
      901 ccgcctgttc gccttccagg caggcgcggc cgtcgaagac cgtgccgcag gcgccgcagc
      961 gcaccaggcc gttgcgcacg cgcaattgat ccgccacgac cttgaaggcc gtgcggcaat
     1021 gcgggcagcg ggtggtgagc gccatggcgc tatcctatct caacgtcggc gttggccgtg
     1081 caggcagacc cagccttcgc gcgcgcgcca gaccgacatg gcgagccagg gcgcataggc
     1141 ggcagccact tcttcggcct ggcgctcgag cacgccggac agcaccagat ggccgcccgg
     1201 cgctacccgc ccggccagca tgggcgccag caccttgagc gggttggaca aaatattggc
     1261 caccaccacc tggaaggcgc cctcgggcag gccgtcgggc agcatggccg acagttcgac
     1321 ccggttgacg gcggcgttgt cggccgtgga ctgcaccgcc tgcgggtcta tgtccaccgc
     1381 cagcgtggcg cccgcgccca gcttgcgcgc ggcgatggcc aggatgcccg agccgcagcc
     1441 gtagtccaac aaggtggcgc cggccggcag ttcggactcg agccaggcca ggcacaggtg
     1501 cgtagtggga tggctgccgg tgccgaaggc caggcccggg tcgagttcga tccggatggc
     1561 atccggggac aagaacgacg gcgcggcggg ggccgccaca tcgtcgcggt gccagctggg
     1621 cacgatccag agcttttcgg caatcttgat ggggccgaac tgcgcctgcg tcaggcgcac
     1681 ccagtcggcg tcgggcacgt cgcgcaagga ccagccctgc cccaggccgg catccagccc
     1741 ggactcggcc aaggcggctt cgagcaactg cgcggggtcg gcgccgtcgg gcagcagggc
     1801 caccacgcgg ttgcggtccc aggcctgcac gtcgggctcg gtgcccggct cgccgaacag
     1861 gggccgctcg ccgtcggtgc cttcgtcggc gtcctcgacg gacaccgaca gcgcgccggc
     1921 ctcgagcagc gcatcggaca gcgcctcggc ctgggcttcc cggcagtgga ggacgagttc
     1981 gcgcatcctc agcctccgcg cggccgcccg gaggaggctg acgccccctt ggggagcagt
     2041 gagcgatagc gagcgtgggg gccgttcatt acgagggacg ctgcgacagc ttctgttcca
     2101 ggtaatggat gctggtgccg ccttccatga agcgggaatc ctgcatcagc tcgcggtgca
     2161 gcgggatgtt ggtctggatg ccctcgacca ccatctcgga cagggcgatg cgcatgcgcg
     2221 ccatcgcctg ctcgcgggta tcgccgtagg taatgacctt ggcgatcatc gagtcgtagt
     2281 tgggcggcac gaaatagccg ttgaaggcat gtgaatcgat gcgcacgccc gggccgcccg
     2341 gcacgtgcca gttggtgatg cggccggggc tgggcgtgaa gcggaagggg tcttcggcgt
     2401 tgatgcggca ctcgatggcg tggcccttga gcacgacgtc gcgctggcgc aggttgaact
     2461 tttcgccggc ggcgatgagg atctgctgct gcacgatgtc cacgccggtg atgagctcgg
     2521 tgacgggatg ctcgacctgc acgcgcgtgt tcatctcgat gaagtagaac tcgccgtttt
     2581 catacaggaa ctcgaaggtg cccgcgccgc ggtagcccat cttgcggcag gcgtcggcgc
     2641 agcggtcgcc cacgcgctcg atcaggcggc gcgcgatgcc gggggccggc gcctcttcga
     2701 tgaccttctg gtggcggcgc tgcatggagc agtcgcgctc gcccagccac acggcgttgc
     2761 gcccgccgtc ggccagcacc tggatttcca cgtggcgcgg attttccagg aacttctcga
     2821 ggtagaccgc ggggttgttg aaggccgcgc ccgcctcgga acgcgtcaag ttgaccgcgt
     2881 tgacgagggc cgcctcggtg tacaccacgc gcatgccgcg cccgccgccg ccgccggcgg
     2941 ccttgacgat gaccgggtag ccgacttcgc gggcaatgcg gacgatttcc tgcggatcgt
     3001 cgggcaactc gccctcggaa ccgggcacca ccggcacgcc ggcttcgatc atggcgcgct
     3061 tggcgctgac cttgtcgccc atcaggcgga tggtttcggg gcgcgggccg atgaagacga
     3121 agccgctctt ttcgacacgc tcggcgaaat cggcgttctc ggacaggaag ccgtagccgg
     3181 ggtggatggc ctcggcgtcg gtcacttcgg ccgccgaaat gatggccggc atgttgaggt
     3241 agctctcgcg cgagggcgcg ggcccgatgc agaccgattc gtcggctagg cgcacgtatt
     3301 tggcctggcg gtcggcctcg gaatgcacga ccacggtctt gatgcccagc tcgcggcagg
     3361 cgcgctgaat gcgcagggcg atctcgccgc gattggcgat caggattttt tcgaacatat
     3421 ttgggagtcg actatcagcc aatgacgaac agcggttggc cgtattcgac cgcatcgccg
     3481 ttttcgacca ggatttcctt gatgacgccg gacttgtcgg cctcgatctc gttgagcagc
     3541 ttcatggcct cgatgatgca cagggcatcg ccttccttga cggtctggcc cacttcgacg
     3601 aagggcgagg cgccggggtt gggcgagcgg tagaaggtgc cgaccatggg cgccttgacc
     3661 gcgtgcccct ggatgaccgg ggcggcaggc gccgcggccg gcgcggcagc gggcgcggcg
     3721 gccggctcgg gcatggggta ggccatgggc tggacagcct gcgagaactt gacgatgcga
     3781 accttgcctt cgccttcggt gatttccagt tccgcgatgc cggactcggc caccaggtcg
     3841 atcagggtct tgagtttacg gaggtccata tgaaggctga ttcccgaatg attgatatgg
     3901 gttgggcagc cccgtcattg ggccgccctc tcatgtgaag agtgaagacc ggcggggctc
     3961 gatcagcggc ccgccgcgta acgcagggcc gccccgtagc cgtcggcgcc caggccgctg
     4021 atcaggccca cggcgatatc ggacaggtac gagtgctgcc ggaacggttc gcgcttaaaa
     4081 aggttggaca aatgtacttc aataaaaggg atggccacgc cggccagcgc gtcgcgtatc
     4141 gccacgctgg tatgggtata ggcccccgca ttgatgatga tgaaatcggt gccgtcgctt
     4201 gccgcggcct ggatgcgatc gaccagggcg ccttcgtggt tgctctgcca ggccgcgagc
     4261 ttgacgccca gttcggccgc caacgccgcc aggccggcat cgatctgcgc cagggtgcgg
     4321 ctgccgtaga tacccggctc gcgggtgccg agcaggttca gattggggcc atgcaggacg
     4381 agtacgcttt gcgccatgag ccatgcctca cagacgtgcc aggcatcagg cattggccca
     4441 accctgagag caaacgtcgt cgaaaataag cgataaaccg cctttttacg ccaaattcac
     4501 ccgcttgtcc aacaaatgcg cctgagaggc ttatccgccg aggtttgtca gcgtgtcgtg
     4561 gacgtcatct ggctggattt ttcccagtat tgatctattt ataccgccat ctgcagccaa
     4621 aatcagggta aacggcaggc cgcccgccgt gtcgcccaag ccacgcaggg tatcgaccgc
     4681 gccggcgccc atgaccagca aggggtagga aaccggcacc ttgaccagaa atttgcggat
     4741 attgtcgaca gtatcgacgc cgatcccgat gaactgggct tgcgggaact gtttttgcag
     4801 ggcgtcgagc tcgggcattt ccttgacgca gggcgcgcac caggttgccc agaaattcag
     4861 caccatcacc cggccctgcc attcggccag acggcgcgtg gcgccctgca ggtcggccat
     4921 ggggcggacc cgcagcgcgg ccagggcatc ggccagcggc ggctcggcgg cgggaaccgc
     4981 ggcctgcgcg gcgcggcctg ccgaagcagc cagcagcgcg gccgccaggc gggcgcccga
     5041 acgaaacaaa tgacggcgat tcatggcggt gatcataccc gcccggcagg gcggcggggg
     5101 gccgcgcctg cctctacaat gcaggcagga gattcatcca tgcacctgca cattctcggc
     5161 atctgcggca ctttcatggg cgggctggcg ctgatcgcgc gctcggccgg ccacaaggtc
     5221 accggctgcg acgccggcgt ctaccccccg atgagcaccc agctggccga acaaggcata
     5281 gacctgatcg aaggctggga cccgtcccag atggcgctaa agcccgatct ctacgtgatc
     5341 ggcaatgtgg tcacccgcgg caatccgctg atggaggcca tcctgaacgc gggcgcgccc
     5401 tacgtgtccg gcccccagtg gctgggcgac cacatcctgc cgggccagca cgtgctagcc
     5461 gtggccggta cccacggcaa gaccaccacc agctcgttcc tggcctggat cctggaatgc
     5521 gccggccgcc ggcccaattt tttgatcggc ggcgtggccc ccgatcttca agtctcggcg
     5581 cgatatgaga gcccccgttc cccgcttgcc gccccccccg gcctcgggac ggcccggcga
     5641 ccgacggacc ttctcgctgc gactttcgtc atcgaagccg acgaatacga cacggccttc
     5701 ttcgacaagc gctccaaatt cgtgcactac cggccgcgca cggccgtcct gaacaacctc
     5761 gaatacgacc acgccgacat cttccccgat ctggccgcca tcgagaccca gttccatcat
     5821 ctggtgcgca ccattcccag ccagggccgc atcgtggcgc ccgcccgtga cgaggccata
     5881 gaccgcgtgc tggcgcgcgg ctgctggtcg gaaaccgtgc ggttcggcgc cgggccggcc
     5941 ccgggcggac acgccctctc ggggggctgc tgggaggccg gcgagccggc gaacgacggc
     6001 gccttcagcg tgcgccgcga cggccaggaa cagggcacgg tgcactggac cctgaccggc
     6061 gagcacaacc gcagcaacgc cctggccgcc ctggcggcgg ccgagcacgc cggcgtgccg
     6121 gcggccgagg gcatcgccgc cctgtcgcgc ttcggcggcg tgaagcgccg catggaactg
     6181 cgcggcacgg tgcgcggcat caaggtgtac gacgacttcg cccaccaccc caccgccatc
     6241 gccaccacgc tcgccggcct gcgccgccag atcgagcgcg ggctcgcgag cgccgggccg
     6301 tccccagcgg gcgatccccc tcggggggac ggcgcacagc gccagggagg caggatactg
     6361 gccgtgctgg agccgcgctc gaacacgatg aagctgggcg ccatggcggc ccgcctgccc
     6421 ggatcgctgg ccgatgccga ccaggtgttc atctacggcg agcgcgaggg caggcatgcc
     6481 ctgggctgga acccggccga ggtgctggcg ccgctgggcg gcaaggcgca cagttacgaa
     6541 cagctggacg aactcgtcgc cgccgtggcc cggaccgcgc gccccggcga ccacatcgtc
     6601 gtcatgagca acggcggatt cggcggcgtg catggaaagc tgctggacgc cctggcacgg
     6661 aaccgcgcag agacatgaag aaccgcattc tctatttgca tggcttccgt tcgtctccgg
     6721 cctcgttcaa gggccggatg atggagcagg ccctggccga acgcggcctg tcgcccatct
     6781 gggccggccc gcagctgccc gccagcccca ggcaagccgc cgacctcgcc ctgcacatcg
     6841 cgcaaacgca gctgcgcgac cgcgccggcc ccaagggcaa ccccgcgaaa ctgaccgtgg
     6901 tgggctcgtc gctgggcggc tactacgcca cctgggtcgc cgaacaactg ggctgcaagg
     6961 ccgtcctgct caaccccgcc gtacacgccg tgcgcgacct caccccggcc gtcggcacgc
     7021 accgcatgta tcattccgac gcgcctttcg tcttccggcc cgaatacctg ggtgaactgg
     7081 ccgcgctgcg cgcgcgccgg atcacccggc cgcgacgcta cttcctcatc gccgccaccg
     7141 gcgacgaagt gctgaactgg cgcgaaatgc gcgaacgtta cgccggctgc cggcaacata
     7201 ttgtgcaagg cagcgaccac gggctatcga acttcgccga tctgctcccc gaagtcctgg
     7261 catttgccct ggaatagttt tcccatgtac gtgttttacg aagatgacgg cagcttcaaa
     7321 gccgcccaca ccatgtccga gacggacgcc acgctgcaga tcgagtccga gtcgggcaag
     7381 cgcagcaaga tcaagcgcgc ggccgtgctc ttctcgttcg acgcgccggc gccggccgag
     7441 ctcatgccgg ccgccgccgc gctggccgaa gacatggacc tgcaattcct gtgggaatgc
     7501 gcgccgcagg aagattttgc cgcgcccacg ctggccgccg attatttcgg ccatgcgccc
     7561 tcgcccgtcg agcaggccgc gctggtgctg cgcctgcatg gcgcgcccgc gtacttccat
     7621 cgccgcggcg ccggccgcta tcgtccggcg ccgcccgaca tcctggccgc cgcgctggcc
     7681 gccatcgaga aaaagcgcct gcaggccgag cagcagcagc aatgggtgga cgacatggcc
     7741 gccgggaggc tgcccgagcc catcgcccag gccgccgaag cgctgctcat caagcccgac
     7801 aagaattccc aggcgtggaa ggcattggac gcggcctgcg cccggctgca gaaaacgccc
     7861 gagcgcctgc tgctggaact gggcgcctgg ccgcacgcgc tggccctgct caagcgccgc
     7921 ttcctggcga cgcacttccc gcgcggcacc ggctttcccg acgtggagat cccgcccatc
     7981 gagcgcgagc tgccgctggc cgacgccgaa atctattcgg tcgacgacgt caccaccacc
     8041 gagatcgacg acgcgctgtc ggtgcgcgtg ctgcccgacg gccgcgtgcg cgccggggtg
     8101 cacatcgccg cgccgggcct ggccgtcacc aagggcagcg aactcgacaa gctggcgcgg
     8161 gcgcgcctgt ccacggtgta catgcccggc gacaagatcc ccatgcagcc cgatgccgtc
     8221 atcagcgcct tctcgctgga cgccggccgc gccgtgccgg cgctgtcgct ctacgtgacg
     8281 gccgacccgg ccacgggcga gatcttcgac accgagacgc gcctggaacg catcgcggtg
     8341 cgcgagaacc tgcgccacaa cctgctcgac ggccaggtca ccgaggcgcg cctggccgat
     8401 ccgcagtccg acctgcccta tgggcactgg ctgcggccgc tgtggcaact ggcgctggcg
     8461 ctctcggcca agcgcgagga agtgcgcggc aagcccgaga acaatacgcg ggtggaatac
     8521 agcttctacc tggacggcaa ccccgacgat cccgacacgc cggtgcgcct ggtgccgcgc
     8581 cagcgcaacg cgccgctcga ccgcatggtg gccgagtaca tgatcctggc gaacaatctg
     8641 tggggcggcc tgctggccca gcacggcgtg ccgggcatct accgctcgca gcaggccggc
     8701 cgcgtgcgca tgagcaccca ggccctgccg cacgaggcca tcggcgtgcc gcagtacgcc
     8761 tggagcacct cgccgctgcg ccgctacgtc gacctggtca accagtggca gctcatcgcc
     8821 gcggtcgaca acggcgtggg cgcccggctg gtggcgccct tcaagcccaa ggacgccgac
     8881 ctctacgcca tcatcggcgc cttcgatgcg caatacgccg cctgggcgga gttccaggca
     8941 agcatggagc gctactggtg cctgcgctgg ctggcgcagc agggcatcac ccgctgtacg
     9001 gccagcgtgc tgcgcgacga cctggtgcgc ctgtccaatg cccccttcgt cacgcgggtg
     9061 ggcggcatgc ccgagctcga gcgcggcacc gtcgtggaaa tcgacatcct gggccgcgac
     9121 gagctggcgc tcgaactcga ctgccgctat atcggcccgg tggcgtagcg cgcatggccc
     9181 ggcgcgacga ctatctgctg atcggcctgg ccatctcgct gatggtgcac gcgggcgcgc
     9241 tggcctggcg cttcgcgccg cgcccggcgc atccggccgc gcccgcgctc gaagtcgtgc
     9301 tggtcaatgc gcacagcgac accgcgccgg cccgcgccca ggcgctggcg cagcagaacc
     9361 tcgagggcgg cggcgaggcc aggcagggcc tggcccgctc gcccctgccg cgcaccggcg
     9421 aatcggccga gaccatcgtg ctgcaggcca tgcacaagcg ccagcaggaa ctcgagcagg
     9481 aacaggcgcg cctgctgatg caattgcaat cgtcccgcca ggccgccgcg ccgtgccagc
     9541 ccgccaaccc cgacgaaacg gccgccgacc ccgggcaaga ccccgaggaa caggccggcg
     9601 tcatccagaa cgcccaggtg gccgccctgt cgcaacgcgt gcaggcctac aacaaggagc
     9661 cgcgccgcgc ctatgtgggc ccggccacgc aggcctcgcg ctacgcggcc tatctcgacg
     9721 ggtggacgcg gcgcgtcgag gccatcggca cgcagcacta tcccgacgac gcgcgcggcc
     9781 acacctacgg ctcgctgcgc atcaccgtgc gcgtcaagcg cgacggcagc ctggccggct
     9841 tcgagatcga ccagccttcg caatacgccg tgctcaacca ggccgcgcgg cgcatcgtgc
     9901 agatggccgc gcccttcccg ccctttccgc ccgacatcgc ccgcgatacc gacgagctcg
     9961 tcatcacccg cacctggcat ttcgtcaacg acacgctgca agcctcagaa agataatgac
    10021 ccccacgccg cgcactccgt gtttgctgcc ccccgagggg gccgcgtttg ccttggggcg
    10081 gcccggcggc aaacggaccc catgaccgac gctccgctcc actacgccgt gatcggcaac
    10141 cccgtggccc acagccgttc gccgcggatc catgccctgt tcggccagca ggccggcatc
    10201 gcgctgcggt acgaccgcct gcccgcgccg ctcgatggat tcgaggccac ggcgcaggcc
    10261 tttgccgagg cgggcggccg cggcctgaac gtgacggtgc ccttcaagcg gcaggccttc
    10321 gatctcgcgc gcggccatct ctccgaccgt gcccggctgg ccggcgccgt gaacacgctg
    10381 tggcggcagg acgaggcctg gcacggctgc aataccgacg gcgtgggcct ggcgcgcgac
    10441 ctggtccgcc tgggcgccag gctggcgggc gcgcgcgtgc tgctggtggg cgcgggcggc
    10501 gccgcccgcg gcgtgctgca gcccctggcc gcggccggct gcgcgcacct ccgcatcgtc
    10561 aaccgcacgc ccggacgcgc ccgcgaactg gcgcagggct ggcaggcggc gtggaccggc
    10621 ctgcccgtgg aagtcagcgc gggcggcctg cccgacgccg ccatcgccgg cggctgggac
    10681 gtggtgatca acgccacggc cagcagcctg cagggcgcgg cgcccgacct gcccgccggc
    10741 ctgtacgccg acggcgcgct ggcctacgac atgctctatg ccgcgcggcc cacgcccttc
    10801 atgcgccagg cggccggcga cggcgcggcg caatgcgccg acggactggg catgctggtg
    10861 ggccaggcgg ccgagagctt cttcatctgg cacggcgtgc gtcccgaccc ggcccccgtg
    10921 ctcgacgcgc tgcgcgccga gctgaccgca cagggctgag cgccatggca agacgcagag
    10981 gtcgccgcgt ccgctggctt gccatgctgg tcatggccct gctgggcctg tgcctgctct
    11041 accaattgtg gatgctgggc caggtgctct ggtattcgcg ctacaacccc ggcagcagcg
    11101 ccgtgatgcg ccaggaactc gcccgcctgc gcaagaccga tccgcgcatc gcgctgcagc
    11161 acgcctgggt gccctacgag cgcatcaacg gcacgctcaa gcgcgcggtg gtcgcatcgg
    11221 aagacgccaa cttcctggaa cacgacggcg tggaatggga cgccatgcgc gaggcctggg
    11281 aatacaacca gcgccaggag gaactgggcc gccggacccg gcgcggcggc tcgaccatca
    11341 cccagcagct ggcaaaaaac ctgttgctgt cgaattcgcg caactacctg cgcaagggcg
    11401 aggagctgct ggtgacgttc atgatcgagc aggtcatgag caagcggcgt atcctcgagc
    11461 tttacctgaa catcgccgaa tggggcacgg gcgtcttcgg cgcccaggcc gcggcgcggc
    11521 actactacca gatcgacgcg tcccagctcg atgcctacca ggccgcgcgg ctggccgcca
    11581 tgctgcccaa cccccgctat tatgatcagc accgcgacac gcgttatctc aactcgcgca
    11641 ccgccaccat cggcgcgcgc atgcgccagg cggacatacc ctagcgccgg cggacccgga
    11701 gcgggcccgc ggatttgtta agcttgcgcg ttccatcgaa attttcatcc ctaccgagtt
    11761 tcacccgtcc tattttcatg cgtaccgccc gtcgctatct ggccctcgag atctaccgct
    11821 cctgcgcggt cgtgctgctg gcgctgctcg ggctgttcac cttcttctcg ctggtggacg
    11881 acctggacgg ggtgggcaag aagttcaccc tgctggccat gctctacatg caggcgctgg
    11941 agacgccctc gcggctctac gacctgctgc ccatcggcct gctgatcggc gccatcctgg
    12001 ccctggcgag cctggcccag cgcaacgagc tggtgatcct gcgcgtctcg ggcgtgagcg
    12061 gcatgcgcct gctcggcacg ctctggctca tcacggtgcc gatgatggcc ggcgcgctgc
    12121 tgctgtccga atacatcacg cccaaggccg agatccgctc cagcgaggtc agcctgatgc
    12181 tgcgcggcca ggccagcggc ggccgcctgg caagcggcta ctggttcaag gagcccacgg
    12241 ccacgggcgg cacccgcatc atcaatatca agaccctgat gggcaacggc gcggtgagcg
    12301 acgtgatggt gtacgaactg acgcccaccc tggagctcag cctggtggcc caggccccct
    12361 cgggcacgct gatgggcggc ggcgacctgg tcctgcgcaa cgtcaccgaa acgcgcctgg
    12421 accccaatgc ctccgaagcg ctggccgacg cccgcccgcc ggcccagccg ccggcgaccg
    12481 tggtcaccga gcccgaactg cacctgtcca ccacgctcaa tccccagcgc ctgctggccc
    12541 gggtgctgac ccccgagcgc atgtccatcc tgtcgctgat cgactatatc gattacctgc
    12601 accgcaacca gctgcaggcc gaccggcaga tcgtggccat gtggcgcaag gcggtctatc
    12661 ccttcacgct gctggtgatg atcaccatcg ccgcccccat cagcttcatg cagacgcggc
    12721 gcggcggcgt gggcgccaag gtgttcatcg gcattttgct gggcgtgggc tttttcatgc
    12781 tcaaccagct ggcgctgaac gtgggcatgc tcagccgctg gccgccctgg ctgacggcgc
    12841 tggggcccaa cgtcggcgct ctgctgctgg cgctggccgc gctgtcggcg atggaatacc
    12901 gcactatcct gagtaatctg gtggcaggcc actggccctg gagaaaagca ctggcatgaa
    12961 cagcagtatc tggatgatcg gcgacgtgca aggctgttgc cagcctctgg agcagttgct
    13021 ctcgcaccct gacataacgg ccgacccgca atcgcagttc tggttcgcgg gcgacctggt
    13081 caaccgcggg ccgcaatcgc tggaatcgct gcgccggatc atgtcgctgg aatcgcgcag
    13141 cgtgaccgta ttgggcaacc acgacctgca cctgctggcc gcggcggccg gggtgcgcaa
    13201 gccgtccaag tcggacacgc tcgacgagat tctcaacgcc ccggacgcgg cggacctgat
    13261 cgattggctg cgcaatcggc cgctggcgca tttcgagcac gaccacctga tggtgcacgc
    13321 cggagtgctg gcgaagtggg acgtggccaa gacgctgtcc ctagcccacg aagtgcagga
    13381 ggcgctgcgc ggcccgaact ggcaaaagtc cctgcagaag atgtacggca acgagccggt
    13441 caactggaag gacggccata cgggcggcaa gcgcctgcgg gtgatcatca atgcgctgac
    13501 gcgcatccgg ttgtgcacgc cgcaggggca tatggatttc aacgccaagg tggcgccggg
    13561 ggcctggccg gacgggctga taccctggtt cgacgggccc aagcgggcga cgcgcggcgt
    13621 gaccatcgtg ttcgggcaaa agaaaagaga gagagagaga gagtataaaa caatagtgga
    13681 aatggtggtg gtttcacaga gttttggttt atggatatcc tttgttctag tgttattgtg
    13741 agattaaatt aggtttaatt gtttaagtct tacgctaggt gtcactttta aaaatattat
    13801 aattcaattg gtatattact cttacacatt attctttatg aaaataatta atatttaaat
    13861 cttctttaaa aaaaaaaaaa aaaaaaaact cttaacaatc gaattatctt aaaaaaaaaa
    13921 ataaagccct acgtggggtc tcttgctttg gcactttttg gcggaaagta acaaatatac
    13981 tgtgttctct taaatttaag ttttacactt ccaaattatt ttatccgtta aaggcggtta
    14041 tgtgcgcaga aactagttaa ccttaaacta tcccatgtta cgtcatcatg tgagtaatcg
    14101 aatctatgga tgatgtatca cggattcata tcccatgcaa tactttgcat gggtgtaatt
    14161 cttttttctt ctcatatttt ttaaattttt tcactttttg ctttttaaaa tttttaataa
    14221 ttttttatca agtggcatac aataccaatt gcatggaata tgaatccata gatcacatgg
    14281 ttggaatcca ataacagctt tcaaccacca atctctctca actatgtgtg tgcatgtgtt
    14341 cgaaattttt gggtttgaaa taagctcaca cactagccat gagaggctaa tgtatcaacc
    14401 ttcgcccaca caaagaaaat gaaccaaaca taaaggaaat taatcttgac tcacaaaatt
    14461 aaacaaagcc aaaatgaaaa gttcacacac acacacac
//
LOCUS       DASWPO010000322        20334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2577598, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000322 DASWPO010000000
VERSION     DASWPO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20334
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577598"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VGC69_16935"
     CDS             complement(<1..998)
                     /locus_tag="VGC69_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0297018.1"
                     /translation="MASSSGLEQAAPFDLTVRGGRIATAADTFEADIGVRDGVIAAIA
                     SRLPAGRQDIDASGNWVLPGGIDSHCHLEQLSGMGVMGADDFHSGTVSAAFGGTTTVI
                     PFAAQHRGMKIPDVIAQYARRAREKAVIDYAFHLIVTDPDEDALSRDLAQAIRDGITS
                     FKVYMTYDLLKLDDYQLLDVLDLARREGALVMIHAENHDMIRWLTRKLLDRGHTAPKF
                     HAVAHAPLAETEAANRAISLARLVDTPVLIVHLASGETVRLVRGARALGAPVHAETCP
                     QYLFLTAGDLDRAGLEGAKFCCAPPPRDGQAQEALWRGLIDGTLRIYSSDHAPYRF"
     gene            complement(1019..1918)
                     /locus_tag="VGC69_16940"
     CDS             complement(1019..1918)
                     /locus_tag="VGC69_16940"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0297019.1"
                     /translation="MQPARYGPFPYAPQPGRPRFELPGGARLALWINPNVEFFGLDDV
                     MPGNVNERVERERTRVPNVRNWSVRDYGNRVGIWRLMDVLSRYGIRASAALNSELCLR
                     HPEIIEEAGRRGWELMGHGITNATRINEMAPEDEREAIFTAVQTIEKVSGTRPVGWLG
                     PGLAETWRTLEYLSEAGIRYVCDWVNDDQPYTMTVGKPPMVSLPYSVQTNDVPAYFDM
                     KVSVPEFEAMIKRQFDVLYREGEQSGRVMAIAVHPFVTGQPHRIGALDAALEYICSHP
                     GVWLATGREIVDHYLQSDFSRAQ"
     gene            complement(1918..2868)
                     /locus_tag="VGC69_16945"
     CDS             complement(1918..2868)
                     /locus_tag="VGC69_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY0297020.1"
                     /translation="MSKCRQIRELLKNSRPLISPGVYDGYSAMLAEAMGFKSISTTGA
                     GLSNSILGEPDIGLFSLRDNIDACRRITHSVSLPVMADADTGYGNAITVYHVVQYFEE
                     AGIVGINLEDQVMPKRCGAMRGKELVEPMEMALKIEAAIKAKKDPDFIINARTDAIAV
                     EGIEGTVKRVREYVAAGADMIYPDAVSSEDDILRVLDAAGDVPVNINMGFGIRRRPTT
                     PLISFKRLAEMGVARISLPRFLPAAALQGMKQAILVLQGSIASGEVVDRPDLLFGIEE
                     ITDLVGYEHIAKLENELLSSEQLQRKYGSQARDYVVKEGH"
     gene            complement(2921..3676)
                     /locus_tag="VGC69_16950"
     CDS             complement(2921..3676)
                     /locus_tag="VGC69_16950"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0297021.1"
                     /translation="MSRLMVDMLNSSVPMYYQIAQVLRHRVESGELGATGKIPTEQAL
                     CEEFGVSRTTVRHALSHLKQEGLLSSRRGVGTQRVATGSLRKKKYVRSSGDPLHAALP
                     SKPRVVSLKQVAPPDAVRDFLGLPAGDKALQIVRVHDLDGEPLSVVVTYLPSFLAEGI
                     TRTALRSSIHEILWNRYGMRQKKSIHTIRIVRADREVASLLNTGLATPVLNIQASTYL
                     EDGAPIRWTENYFREDRYEYVAEFLWHKPPRRR"
     gene            3757..4743
                     /locus_tag="VGC69_16955"
     CDS             3757..4743
                     /locus_tag="VGC69_16955"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEY0297022.1"
                     /translation="MSAAEVPDFDAIPVIDMSPLFSDASHEAVAKVAARIRKAAVEIG
                     FFYVSGHNVSRDLQQAVYLCSKYFFCQPDETKAAIAINNAHRGYVGFRQTVLHSARKA
                     DLKESFNFAYPFKEGDPELDKGHGLVGLNQWPRGEDVWRDVVERYYGEVFELGQRILE
                     GFALALGTRRDFFRDLYRHPLVRARLLHYPPQTVDSQSGQFGAAEHTDYGCITLLWQD
                     DVGGLQVKNRAGKWVDAPYIEGTFVINIGDMLARWSNDLFVSTPHRVVNDSGRERYSI
                     PVFYDPDYETEVECLPNCATAESPPKYPRIVAGDYITDKYDTSYAYRQKGRG"
     gene            complement(4740..5624)
                     /locus_tag="VGC69_16960"
     CDS             complement(4740..5624)
                     /locus_tag="VGC69_16960"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297023.1"
                     /translation="MLRLIVPLAPGGGTDAVSRLVAVEMGKNLGQAVVVENQSGGSGT
                     IGMMTVVRAEPGGYTLLTGTPSLSIDPSLRRDMRFDPLEDLRPVCLFSRVPYVLVSSM
                     TFQPRTIAALLDKARASPGSITFGSPGIGSGGHLAGELFQLLAGVKLTHIPYKGSGPA
                     MNDLRGGRIDLLFATTPAITQFVKAGALRPLAVSGARRTHELPDVPTIAESGIPGYET
                     YSWYGLWTTAKTPDAVVARLNLAVRTALSQPDVLRAIEADGGEPKPTTPKQTGDFLAQ
                     EIDKWRMVIKKAAITVND"
     gene            complement(5769..6917)
                     /locus_tag="VGC69_16965"
     CDS             complement(5769..6917)
                     /locus_tag="VGC69_16965"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HEY0297024.1"
                     /translation="MDTPARGDARQRAIRCIIMRGGTSKGIYFHESDLPAEPKARDAL
                     LLAVMGSPDPRQIDGLGGADTLTSKIVIIGPSTRPDADVNYTFGQVGIDLPYVSYSAN
                     CGNLSAAVGVFAIEEGLVAPVHPVTRVRIYNTNTQQLLVSYVPVDESGQPRIDGDCAI
                     AGVPGTAAEIRMDFSATLGASTGKLLPSGHLTDALHVPELGREIPVSIVDAAKATVFF
                     HARDAGILGTESPEQFTPEILGRFWAIRNAAAALVGLSPESRLPHPVSVLAPTDYINH
                     MTKEIVPAQDLSFVARRVGGPPPRLHKAFAATGAVCTAIAANLPGTVVHEVARRFPDG
                     MVRIGHPTGVFPVRIALADNGEIREASYVRTARRIMEGTVYVREPAGL"
     gene            complement(7050..7850)
                     /locus_tag="VGC69_16970"
     CDS             complement(7050..7850)
                     /locus_tag="VGC69_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0297025.1"
                     /translation="MEDETSRGTQVIRRVATLLRLLTAHNRTGMRLVDLCRAAEIERS
                     TVHRMLQGLIAEHLVSQDRDSKRYFLGIGVYEMGVAIAPPVQLRDVCQPYMNALAEQT
                     GDTVFLTVRSGFDGVCVDRKEGSFPVKAFVLEPGKRRPLGIGAGNIAILSGLPGDEIT
                     RICLANRARIEQLHPRYTEDELATRIERSQTLGYTVTDVLETTGVRSVGMRIRDRNHL
                     PIAAISVSAVSSRLENERVQEVATFLRAAVDALEEKLKHMSVEDTHHL"
     gene            8007..9395
                     /locus_tag="VGC69_16975"
     CDS             8007..9395
                     /locus_tag="VGC69_16975"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0297026.1"
                     /translation="MSGLTQAMARTVVERGFDGLSPQAVDKAKKVITDTFAVILSGAG
                     SEVEEPLMKYLDMSQSPGRSPILGTARRTGAETAALINGTFGHALDFDDVLSMMPAHP
                     SAIIVAALMADLADNPLSGREFIEAYAIGVEVGARIAQGITVGHYGRGFHGTGTLGTF
                     SALAAMAKARRLDLPTTQIAFGIAASMASGVRRNFGTMTKPLHTGLAARNAVQAVRLA
                     MCGFTAAPDALEAKAGFYAAYGVESSDPAVAIAALQGGSVLLDPGIALKKFACCYASH
                     RGMDGVLALRQKHGFTAADVVRLECRMPPGGMLVLTYPAPTTGLEGKFSLQYALAAGA
                     LDGKYSIWSFTDEAVRRPEIRELLTRISAFEDESCRDDDPLFDTRSSGGRGFVEVEVE
                     LRDGTRDCVRVNKAPGHPSRELSWDDIAAKFIDCARHAGLPQESAGEALASLRELESC
                     RDLGGLIGKLHH"
     gene            9497..10747
                     /locus_tag="VGC69_16980"
     CDS             9497..10747
                     /locus_tag="VGC69_16980"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0297027.1"
                     /translation="MQSVVSPGALEGIRVLEIAGAAGQYCGKMFAGLGADVILVEPPG
                     GAGIRNQAPYVGGLEAGDREAVRSLSFAYFNCGKRGITLSLRDREGQALFHRLAAGAD
                     VVIDSNRPGALAEMACGFEQLLALNPALVMTSITPFGQDGPYSAYEADDLVCLAAGGF
                     LYLGGYPDSEPIGAYGDQAYLGGSMFGAVATMLALTQAEAGGQGEHVDVSMQECMVLA
                     MENAVQFYDLEGMVRKRSAGIQRFAGTGVYECMDGHIYMMAGGIGANRFWGRTIEWLV
                     AEGVPDVQRLYGEQWNETAYLQSDEAKRIFMEVFGNWAKTKTKGYLYREGQNRHVPAA
                     AINTVEDLVASPQLNARGHFVPLAQPGWPKPGLMPGAPYQLARTPWAIASPAPLPGQH
                     NAELYGELGLSSDDRARLAGRGVL"
     gene            10747..11964
                     /locus_tag="VGC69_16985"
     CDS             10747..11964
                     /locus_tag="VGC69_16985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0297028.1"
                     /translation="MRKLPLSGIRVTDFTWIGAGSYTTKILADAGADVIKIESADRID
                     SLRLAAPYKDGIKGENRSGYFADRNSSKRSLTLNMKHPRALELVTALIRQSDIIANNF
                     TPGVMDKFGLGYDAVRGIKPDIIYLAMSMQGGTGPESHYLGYGATMAAVTGIQQLTGL
                     PGREPAGTGTNYPDHIPNPCHAAFVLLAALRHRRRTGQGQRIDMAQTEPTVALLGPAV
                     LDYTVNGCVPQPRGNRHPGAAPHGVYPCAGEDRWVAVSVMNDAQWQALYKALGDPDWM
                     ADPALLPAAERVARADVLDAHLARETARFEADRLVRLLQAHQVPAALASTAADIIEDP
                     QLAHRRHWLTLDHPEMGPSLYNAQPFRYARGAAADARPAPLLGQHTRDVCHELLGLSD
                     GEIERLTREGALK"
     gene            12010..12999
                     /locus_tag="VGC69_16990"
     CDS             12010..12999
                     /locus_tag="VGC69_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY0297029.1"
                     /translation="MRIAILDDYQNLALQLADWSPVRARGDIVVFDRHLAEEEAAAAL
                     RDFDVLCVLRERMPLPGSLLERLPRLKFIAATGPYNRTLDLRAADRLGITVSATPRAA
                     AGQFATAELAWALLLALARNIPLEAANMRVGGWQKTMGLSLSERTLGLVGLGRLGSHM
                     APIARAFGMRVIAWSQNLTAGAAEALGAQRVEKDELFARSDFVSLHLVLGERTRHIVG
                     RRELGLMKPEAYLINTARGALVEREALIDALRARRIAGAGLDTFDIEPLPADDPLRAL
                     DNVILTPHLGYTVEELLGMFYASTVENVLAYLDGKPLRVLNEASPRLTGQVAV"
     gene            13037..14032
                     /locus_tag="VGC69_16995"
     CDS             13037..14032
                     /locus_tag="VGC69_16995"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297030.1"
                     /translation="MNEQRRRFSQTALALASTAVLAPLYASDEPASKFPSHSMYLMIP
                     FEPGGGTDIVGRAIAQKTSEALGEAMVVENRGGANGTIGASVVARSEPDGYTLCMFTA
                     SHSVNVTLQGRQQPYDLLRDFAPLIEIAVQPYILVVRPTLPVRSVGELIALAKSRPGG
                     LTFGSSGVGGLLHLSAELLSTLAGIKLTHVPYKGGSQAMLDVMAGNVDMMFTSLNQSK
                     SYIASGQLRLLAVTSPKRSPAAPDIPTMQEAGVPGYDVQSWYGFAVPAKVPAPIIDKL
                     NRTINKVLALPEVQRQMAIDGSTPVGGSPADFGRFLSAEVVKWRDLIHQVGIPVG"
     gene            14181..15437
                     /locus_tag="VGC69_17000"
     CDS             14181..15437
                     /locus_tag="VGC69_17000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0297031.1"
                     /translation="MDGVDSKAATSPPGALEGVRVLDLSGPLGNYCGKLFAEMGADVI
                     LVEPTGGTRARFEPPFLDASPGLERSLSFAYYNTSKRGVTLDLDCTSGQGLLRRLAAR
                     ADIVIETEKPGKMRERGLAYDDLIRVNPSLVYTSITPFGQSGPYAQYAAEDINGLAMG
                     GLLYLGGYADTPPMRMHGNQGILCANMYASVATMLALLHQEKSGQGQQVDVSMQECIV
                     MALENSVQFFDLEQTVRKRHAGEQRFAGTGVFACQDGDIYLMAGGIGANKFWDRTIDW
                     FAEEKMAGVERLRQEEWARIEYLRSDEAKHIFAELFIPWARQRSKDYLYREGQRRRIP
                     IATISETSDLLGSPQLAFREYFTKVAHPLREEPLLMPGAPYKLSETPWRIQRPAPMLG
                     EHNAEIYSEIGVDSSELEQLFAAGVI"
     gene            15434..16657
                     /locus_tag="VGC69_17005"
     CDS             15434..16657
                     /locus_tag="VGC69_17005"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0297032.1"
                     /translation="MKDEKILEGIRITDFCWLGAGAYATKTMADFGADVIKIENASRI
                     DSLRMAAPYKDKQPGVNRSGYFADRNTNKRSLTLDMKNPRAMALIRRLIEQSDVVSNN
                     FTPSVMEKFGLSYQQVREIKPDIIYLAMSMQGTQGPERDYLGYGASMVALTGLQHLTG
                     LPEREAAGPGTNYPDHVPNPCHAAFALLAAIRHRRRTGQGQFIDLAQIEPTIALLGPT
                     VLDLTVNGRNHNRDGNLHPYAAPHGAYPGAGQDRWLTIAVMNDAQWDALVQAMDLPAW
                     AHDERWRDGAYRHRNRAELDTLLAGETAGWVAEELMAVLQARGVPAGVVQNTEDVVTH
                     DPQLRSRGHWVRLRHAEMGETLYNAPPFRFSEISTSLRRPAPLLGEHTREVCADLLGL
                     SDGEIDELMAQGVLQ"
     gene            16691..17521
                     /locus_tag="VGC69_17010"
     CDS             16691..17521
                     /locus_tag="VGC69_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0297033.1"
                     /translation="MNLEQARRFIADTEQAGDVLRFPCHGGQAVWHRFGQGRPLVMLH
                     GGHGSWLHWIRNIQALSRAHEVWLPDMPGFGGSAEIPVHGMDGLVQAMLAMLDEAFAD
                     REIDLAGFSFGGLVAANIAAARPGRVRKLALLGSAGHGGPRRQTVDLVNWRHSTSQEA
                     LHAVMRGNLAAHMLADPAAIDGLALTAHIRSCEQVQFRSKGISRTWKLDDALRRVQAP
                     VLMMWGEHDVTADPALILPGLTEGHALREGLILPGGGHWIQFESADAVDARLEAWFAG
                     "
     gene            complement(17555..18340)
                     /locus_tag="VGC69_17015"
     CDS             complement(17555..18340)
                     /locus_tag="VGC69_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0297034.1"
                     /translation="MPAKPTPESASGTQAIKRAVSLLRLIASNNRTGMRLVDLYTAAG
                     LERPTAYRLLQGLISENMVRQDAKSRRYFLGRLMYEMGGSAAPQTALRDICHPYLQSI
                     AERTGDTVFLTVRSGLDGVCLDRVEGAFPVKVFVLDIGRRRPLNVGGGAIALLSALPD
                     AEVDKILKGNQAYVREKYPNYAEKKLRARIATARSQGYVQNDLLEVPGVSSIGVAICK
                     RDHTPVGAISVSALASRLSGDRAKLVASYLIEAASNIQSALDG"
     gene            complement(18420..18833)
                     /locus_tag="VGC69_17020"
     CDS             complement(18420..18833)
                     /locus_tag="VGC69_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY0297035.1"
                     /translation="MSEYLKPLPALNKDNSPFWEGCKEGKLRMQRCDACGHIRFPINT
                     VCPRCLDSGFHWHDLSGRATVFSYIVFHQVYNKAFAGDVPYNVALVQLEEGPRMYSNV
                     VGVPNDAVKVGDALEVVFDPVTPEVTIPRFRLRGQ"
     gene            complement(18837..19982)
                     /locus_tag="VGC69_17025"
     CDS             complement(18837..19982)
                     /locus_tag="VGC69_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0297036.1"
                     /translation="MHPLRERTAIAGVGASPQGKFPEETPITLGIAAVKRALDDSGLK
                     KEQIDGLLTMPGTTSPEAALNYLRLGEALGINPRYTGSKNMGGGTAGALLHEAALAVN
                     AGFANYVACVFGDTAATGGNKFNRASGWGDSWGIWGFMAACANSAITASRHMALYGTT
                     SRQLGEIAVACRRHASMNPNAIMRTPITLDDHQNSRWIVEPLHLLDCCLISDGAVCVI
                     VTTAERAKDLKQPLVTIAGMGQGYTTENMEREDWWYVPHQKLAVNDAYAMAGVGPRDI
                     DVCQLYDNFTSSVLVWFEHAGFCKPGEAGPFVEGGRIQLGGELPINTAGGNLSESYME
                     GWLHIVEGVRQMRGECGPRQVAGAEVCLVTGRGQALNCANATILRKG"
     gene            complement(20056..>20334)
                     /locus_tag="VGC69_17030"
     CDS             complement(20056..>20334)
                     /locus_tag="VGC69_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0297037.1"
                     /translation="DAAEAHRIGLVSKVVAQAELLAAAHDIARTIVSNAPLSVRAVKR
                     MVRQAEDMPLPYAMDAERALFGLLYNSEDRIEGRKAYAEKRKPRFKGR"
BASE COUNT         3441 a         6420 c         6899 g         3574 t
ORIGIN      
        1 tcgaagcggt agggcgcatg gtcggacgag tagatgcgca aggtgccgtc gatcaagccg
       61 cgccacaggg cttcctgcgc ctggccgtcg cgcggcggtg gggcgcagca gaacttggcg
      121 ccttccaggc cggcgcgatc caggtcgccg gcggtcagga agaggtactg cggacaggtc
      181 tcggcgtgca ccggcgcgcc cagggcccgc gcgccgcgaa ccagccgcac ggtttccccg
      241 cttgccaggt gcacgatcag caccggcgta tcgaccagcc gggccaggga gatcgcgcgg
      301 ttggcggcct cggtttccgc cagcggggcg tgcgccacgg cgtggaactt gggcgcggtg
      361 tggccgcgat ccagcagctt gcgcgtcagc cagcggatca tgtcgtgatt ctcggcgtgg
      421 atcatcacca gcgcgccctc gcgccgcgcg agatccagca cgtccagcaa ctggtaatcg
      481 tcgagcttga gcaggtcgta ggtcatgtag accttgaacg aggtgatgcc gtcccggata
      541 gcctgcgcca ggtcgcgcga gagcgcgtcc tcgtccggat ccgtcacgat caggtggaag
      601 gcatagtcga tgacggcctt ttcccgggcg cgccgggcgt attgcgcgat gacgtcggga
      661 attttcatgc cgcgatgctg cgcggcgaac gggatcaccg tcgtcgtgcc gccgaacgcc
      721 gcggacaccg ttccggaatg gaagtcgtcc gcgcccatga cgcccatgcc cgacaactgc
      781 tccaggtggc agtggctgtc tatgccgccg ggcagcaccc aatttcccga cgcgtcgatg
      841 tcctgcctgc cggcgggcag gcgcgaggcg atcgcggcga tgacgccatc gcgcacgccg
      901 atgtcggcct cgaaggtgtc ggcggcggtg gcgatacggc cgccgcgcac cgtcagatcg
      961 aacggcgccg cctgctcgag tccggaagaa gaggccatgg gacgcgtcac gataggctct
     1021 attgcgcgcg ggaaaagtcg gactgcagat agtggtcgac gatttcccgg ccagtcgcca
     1081 gccagacgcc tgggtgcgag cagatgtatt ccagggcggc atccagggcg ccgatgcggt
     1141 ggggctgccc ggtgacgaag ggatgcacgg cgatcgccat gacgcgtccc gactgctccc
     1201 cctcgcgata cagcacgtcg aattgccgct tgatcatggc ctcgaactcg ggcaccgaga
     1261 ctttcatgtc gaaataggcc ggcacatcgt tggtttgcac cgagtagggc agcgacacca
     1321 tgggcggctt tcccacagtc atcgtgtagg gctggtcgtc gttgacccag tcgcacacgt
     1381 accggatgcc ggcctcggac aggtattcca gcgtgcgcca ggtctcggcc aggccggggc
     1441 cgagccagcc cacgggccgc gtcccgctga ccttttcgat ggtctggacc gccgtgaaga
     1501 tggcctcgcg ctcgtcttcc ggcgccattt cgttgatgcg cgtcgcgttg gtgatgccgt
     1561 ggcccatgag ctcccagccg cgccggcccg cttcctcgat gatttccgga tggcgcaggc
     1621 agagttcgct gttgagcgcc gcgctggcgc gtatgccgta gcggctgagc acgtccatca
     1681 ggcgccagat cccgacgcgg ttgccataat cgcgcaccga ccagttgcgc acattgggca
     1741 ccctggtgcg ttcgcgctcg acgcgttcgt tgacgttgcc gggcatcacg tcgtccagtc
     1801 cgaagaactc cacgttcgga ttgatccaca gcgcgagccg ggcgccgccg ggcagctcga
     1861 accggggccg cccgggctgg ggcgcatagg gaaagggacc gtatcgggcg ggttgcatca
     1921 gtgcccctcc ttgaccacgt agtcgcgcgc ctgcgacccg tacttgcgct ggagctgctc
     1981 gctcgaaagc agctcgtttt ccagcttggc gatgtgctcg tagcccacca ggtcggtgat
     2041 ttcctcgatg ccgaaaagca ggtccggccg gtccaccact tcgcccgagg cgatgctgcc
     2101 ttgcaggacc aggatggcct gcttcatgcc ttgcagggcg gcggcgggca aaaatctcgg
     2161 caagctgatg cgcgccacgc ccatttccgc caggcgcttg aaggagatca gcggcgtggt
     2221 cgggcggcgc cggatgccga agcccatatt gatgttgacc ggcacgtcgc ccgcggcgtc
     2281 cagcacgcgc aggatgtcgt cttcgctgct gacggcgtcg ggatagatca tgtcggcccc
     2341 ggcggccacg tattccctga cgcgcttgac cgtgccctcg atgccttcga cggcgatggc
     2401 gtcggtgcgc gcattgatga tgaaatccgg gtctttcttg gccttgatgg cggcttcgat
     2461 cttcagcgcc atttccatgg gttcgaccag ttccttgccg cgcatcgcgc cgcagcgctt
     2521 gggcatgacc tggtcttcga ggttgatgcc gacgatgccg gcctcttcga agtattgaac
     2581 gacgtggtag accgtgatgg cgtttccgta tcccgtgtcg gcatcggcca tgacgggtag
     2641 ggacaccgaa tgcgtgatgc gccggcaggc gtcgatgttg tcccgcaggc tgaacaagcc
     2701 gatatcgggc tcgcccagaa tggaattcga aagaccggcg ccggtggtgg agatcgactt
     2761 gaaccccatg gcctcggcca gcatggcgct atagccgtcg tacacccctg gggaaatcaa
     2821 ggggcgggaa tttttcaata actcgcggat ttgtcggcat ttgctcatga tcgggttctc
     2881 tcaaatggcg tggaacggcg cgacgccgca tgggccttca tcaccgtctg cgcggcggtt
     2941 tgtgccaaag gaattcggcg acgtattcat agcggtcttc gcggaaatag ttttcggtcc
     3001 accggatcgg cgcgccgtct tccaggtaag tcgatgcctg gatgttcagg accggcgtcg
     3061 ccaacccggt gttcagcagc gaggccactt cgcggtccgc gcgcacgatg cgtatggtgt
     3121 ggatgctttt tttctggcgc atgccatagc ggttccacag gatttcatgg atggagctgc
     3181 gcagcgcggt gcgggtgatg ccctcggcaa gaaaactcgg caggtaggtg acgaccacgg
     3241 acagcggctc tccgtccaga tcgtgcacgc gcacgatctg cagcgccttg tcgccggcgg
     3301 gcaggcccag gaaatcgcgc acggcgtcgg gaggcgcgac ctgtttcagc gacaccactc
     3361 tgggtttgct cggcaaggcc gcgtgcagcg gatcgcccga cgagcgcacg tatttcttct
     3421 tgcgcaggga acccgtggcc acccgctgcg tgcccacgcc tcgccggctg ctgagcaggc
     3481 cttcctgctt caggtggctc agcgcatgcc ggacggtggt gcggctgacg ccgaattcct
     3541 cgcacagcgc ctgctcggtg ggaatcttgc cggtcgcgcc gagctcgccg gattcgacgc
     3601 ggtgccgcag cacctgggcg atctggtagt acatgggaac gctcgaattc agcatgtcga
     3661 ccataagcct ggacatttgt atctggttaa ttacaatgag acaaatctaa ccgtcattgc
     3721 gtatccgtgt caatcgcctg ccaggagaag gtccagatgt ctgccgctga agttccggat
     3781 ttcgatgcca ttcccgtcat cgacatgtcg cccctgtttt ccgacgcaag ccacgaagcc
     3841 gtcgccaagg tggccgcgcg aatacgcaag gcggcggtgg aaatcggctt cttctatgtg
     3901 agcggacaca atgtttcccg ggacctgcag caggccgtgt atttgtgttc caagtacttt
     3961 ttctgccaac ccgacgaaac caaagccgcc atcgccatca acaatgcgca tcgcggctac
     4021 gtgggattcc ggcagaccgt gctgcacagc gcccgcaagg cggatctcaa ggaaagcttc
     4081 aatttcgcct atcccttcaa ggaaggcgat cccgaactcg acaaggggca tggcctcgtc
     4141 ggattgaacc aatggccgcg aggggaagac gtctggcgcg acgtggtcga gcgctactac
     4201 ggcgaggtct tcgaactggg gcagcgcata ctggaaggtt tcgccctggc cttgggaaca
     4261 cggcgcgatt tcttccgcga tctttaccgc catccgctgg tgcgcgcgcg gctgctgcat
     4321 tacccgccgc agacggtcga tagccagtcg ggccaattcg gcgcggccga gcacacggac
     4381 tacggctgca tcaccttgct ctggcaggac gacgtgggcg gcctgcaggt gaagaaccgg
     4441 gccggaaaat gggtggacgc gccctatatc gaaggaacct tcgtgatcaa catcggcgac
     4501 atgctggcgc gctggtcgaa cgacttgttc gtttccaccc cgcatcgcgt ggtcaacgat
     4561 tcaggccgcg aacgctattc gatccccgtc ttctacgatc cggattacga aacggaagtc
     4621 gaatgcctgc cgaattgcgc caccgcggaa agcccgccga agtatccgcg catcgtggcg
     4681 ggagactaca tcacggacaa gtacgacact tcttacgcct atcggcagaa gggtcgaggc
     4741 tagtcgttca ccgtgattgc ggccttcttg atgaccatgc gccacttgtc gatttcctgg
     4801 gcaaggaaat cccccgtctg cttcggcgtc gtgggcttgg gctctccgcc gtcggcctcg
     4861 atggcgcgca ggacgtcggg ctggctgagc gcggtgcgta ccgccagatt caagcgcgcg
     4921 accacggcgt cgggcgtttt cgcggtggtc cacaagccgt accacgaata ggtttcatac
     4981 cctggaatgc cgctttcggc gatggtcggc acgtcaggca gttcatgtgt gcgcctcgcg
     5041 cccgatacgg cgagcggacg cagcgcaccc gccttgacga attgcgtgat ggcgggcgtt
     5101 gtggcaaaga gcaggtcgat gcgtccgccg cgcaggtcgt tcatggccgg cccggatcct
     5161 ttgtacggga tatgcgtgag cttgacgccg gcaagcagct ggaacagctc gccagccagg
     5221 tggccgccgc tgccgatgcc gggcgacccg aaggtgatcg aacccggtga ggccctggct
     5281 ttgtccaaca gcgccgcgat ggtcctgggc tgaaatgtca tggacgacac cagcacgtac
     5341 ggaacgcggc tgaacaggca aacgggccgc aaatcctcga gcggatcgaa tcgcatgtcg
     5401 cgccgcaatg acgggtcgat cgacaaggac ggcgtgcccg tcagcagcgt gtagccgccg
     5461 ggttcggcac ggaccacggt catcatgcct atggtgccgc tgccgccgct ctgattttcg
     5521 acgacgacag cctgacccag attcttgccc atttcgacgg caaccagccg cgaaaccgca
     5581 tcggtccctc cgcctggcgc caggggcacg atcaagcgca gcatatgcgt tggaaaatcg
     5641 gccgccgcat gcgaaagacg cggccaggcc gcgctggaaa aagcggcagc cgaagccgac
     5701 atgagaaatg atcttctgtc cacgatgtct cctggtgttc tgccagtttt cttatggttt
     5761 tctttgaact aaaggccggc cggttcgcgc acgtataccg ttccctccat gatccgccgc
     5821 gccgtgcgca cgtaggaggc ctcgcggatt tcgccgttgt cggcaagggc gatgcgaacg
     5881 gggaaaacgc ccgtggggtg cccgatgcgg accatgccat ccggaaagcg gcgcgcgacc
     5941 tcgtgcacca cggtgccggg cagattcgcc gcaatcgcgg tgcatacggc gccggtcgcc
     6001 gcaaaggctt tgtgcaatcg gggcggcggg ccgccgaccc gccgcgcgac gaaactgaga
     6061 tcttgcgcgg gcacgatctc cttggtcatg tggttgatgt agtccgtggg cgcaagcacg
     6121 gacacggggt gcggcaaccg ggattcgggg gagaggccca ccagggcggc cgcagcgttg
     6181 cgtattgccc agaagcgccc gaggatctcg ggcgtgaatt gctccgggct ttccgtgccc
     6241 aggatgccgg cgtcgcgcgc atggaagaac acggtggcct tggcggcgtc cacgatggag
     6301 acggggatct cgcggccaag ctccggcaca tgcagcgcat cggtcaggtg gccgctgggc
     6361 agcagcttgc ccgtactggc gcccagggtc gcggagaaat ccatgcggat ttcggcggcg
     6421 gtgccgggta cgccggcgat cgcgcagtcg ccgtcgatgc gcggctggcc gctttcgtcg
     6481 acgggcacat aagacaccag caactgttgg gtgttggtgt tgtagatccg gacgcgggtg
     6541 acgggatgca cgggcgccac caggccttct tcgatggcga agacgccgac ggccgccgac
     6601 agattgccgc aattggcgga gtacgagacg tagggcaggt cgatgccgac ttgcccgaag
     6661 gtgtaattca cgtcggcgtc gggccgcgtg ctcgggccga tgatgacgat cttgctggtc
     6721 agcgtgtcgg cgccgcccag accgtcgatc tggcgcgggt cggggctgcc catgacggca
     6781 agcagcaagg catcgcgcgc cttgggctcg gcgggcaggt ccgactcatg gaagtagatg
     6841 cccttgctgg tgccaccgcg catgatgatg cagcggattg cgcgttggcg ggcgtcgccg
     6901 cgtgccggcg tatcaatggc tgtctcctgg gtgttcggcc ccgcgccggt ggcgcggcgg
     6961 ctcgtttatt cgctccagga aacataccag gacccgcgaa cgcacagcgc acaaagtcca
     7021 tattgcggac gcgtatgcgc ccgccgcgct catagatggt gcgtatcttc gacggacatg
     7081 tgcttgagct tttcttccag ggcgtcgacg gcggcccgca agaaggtggc gacctcttgc
     7141 acccgttcgt tttccagccg tgaagaaacg gccgaaacgc tgatggcggc gatgggcagg
     7201 tggttgcggt cccggatgcg catgccgacg gagcgcacgc ccgtggtttc cagcacatcg
     7261 gtgacggtgt agcccaaggt ttgcgagcgc tcgatgcgcg tcgccagttc gtcctcggtg
     7321 tagcgcgggt ggagctgttc gatgcgggcg cggttggcca ggcagatgcg cgtgatttca
     7381 tcgccgggca ggccgctcag gatggcgatg ttgcccgcgc cgatgcccag cggcctgcgc
     7441 ttgccgggtt ccagcacgaa ggccttgacc gggaagctgc cttccttgcg gtccacgcat
     7501 acgccgtcga agcccgagcg caccgtcagg aacaccgtgt ctccggtctg ttcggcgagc
     7561 gcgttcatat agggctggca gacatcgcgc agctgcacgg gcggcgcgat ggccacgccc
     7621 atttcgtaga cgccgatgcc caggaaatag cgtttggaat ccctgtcctg ggaaaccagg
     7681 tgttcggcga tcaggccttg cagcatccgg tgcacggtgg agcgctcgat ctcggcggcg
     7741 cggcacaggt cgacgagccg catgccggtg cggttgtggg cggtcagcag ccgcaacaac
     7801 gtcgccacac ggcggatgac ctgggtgcct cgagaggttt cgtcttccat ggttgtcctc
     7861 tttatgcgcg ccacgcggcg attcccacta cgtggaacat tttctgggac caagggcgtc
     7921 tcccctccct tgattggtct taccatgacc acattatgag ctattaagct gacttcagaa
     7981 atcagcaaaa gcacaggagg agaggcatgt caggcttgac gcaagccatg gcccgcacgg
     8041 tcgtggaaag aggcttcgac ggtttgtcgc cgcaggcggt ggacaaggcc aagaaggtca
     8101 tcaccgacac gttcgccgtg atcttgtcgg gcgcgggcag cgaggtcgag gagccgctga
     8161 tgaaatacct cgacatgtcg cagtcgccag gccgttcgcc gatactcggc acggcgcgcc
     8221 gcaccggcgc ggagacggcg gcgctgatca acggcacgtt cggccatgcg ctggatttcg
     8281 acgatgtgct gtcgatgatg ccggcgcacc ccagcgccat catcgtcgcc gcgctgatgg
     8341 ccgacctggc cgacaacccg ctgagcggac gcgagttcat cgaggcctac gcgatcggcg
     8401 tggaggtcgg cgcccgcatc gcgcaaggca tcacggtggg ccactacggc cgcggctttc
     8461 acggcacggg cacgctgggc acgttttcgg cccttgccgc catggccaag gcgcgccggc
     8521 tcgacctgcc gaccacgcag atcgcgtttg gcatcgccgc gtcgatggcc agcggtgtgc
     8581 gccggaactt cggcacgatg accaagcccc tgcatacggg gctggccgcg cgcaatgccg
     8641 tgcaggccgt gcgcctggcc atgtgcggct tcaccgcggc gccggatgcg ctggaagcga
     8701 aggcggggtt ctatgcggcc tatggcgtcg agtcctccga ccccgccgtg gccattgccg
     8761 cgctgcaggg cggcagcgtg ctgctcgacc ccggtattgc attgaagaag ttcgcctgct
     8821 gctatgcctc gcatcgcggc atggacgggg tgctggcgct gcgccagaag cacggtttca
     8881 ccgccgccga tgtggtgcgc ctggaatgcc ggatgccgcc cggggggatg ctggtgctga
     8941 cgtaccccgc gcccacgacg ggattggaag gcaagttcag cctgcaatat gccctggcgg
     9001 ccggcgccct cgacggcaag tattcgatct ggtcgttcac cgacgaggcg gtgcgccgcc
     9061 ccgagatccg tgaactgctc acgcgcatct cggccttcga ggacgaatcc tgccgcgacg
     9121 acgatccgct tttcgacacc cgcagctcgg gcgggcgggg ttttgtcgag gtcgaggtgg
     9181 aattgcgcga cggcacccgg gattgcgtgc gcgtcaacaa ggcgccagga catccatcgc
     9241 gcgaactgag ctgggacgac atcgccgcga agttcatcga ctgcgcccgg catgccggct
     9301 tgccgcagga gtcggccggc gaggccttgg ccagtttgcg cgaactggaa agctgccgcg
     9361 atctgggcgg actgatcggc aagctgcacc actagcaccc gcgaggagtt tcttttgagg
     9421 tcccggggcc ggcgccgcgc gcaggcgccc ggtggtttgt cacgtccttg aggttccgag
     9481 atcgatgagt ccatccatgc agagtgtggt gtctcccggc gcgttggaag gtatccgcgt
     9541 cctggaaatc gcaggcgccg cggggcagta ttgcggcaag atgtttgcgg gcctgggggc
     9601 cgacgtcatc ctggtcgagc cgcctggcgg cgcgggcatc cgcaaccagg cgccgtatgt
     9661 cggcgggctg gaggcgggcg acagggaggc tgtgcgcagc ctgtcctttg cctatttcaa
     9721 ctgcggcaag cggggcatca ccctgagcct gcgcgaccgc gaaggccagg cgctgttcca
     9781 ccggcttgcg gcgggcgccg acgtcgtgat cgacagcaac cggcccggcg cgctggccga
     9841 aatggcttgc gggttcgagc agcttttagc gctgaacccg gcgctggtga tgacgagcat
     9901 cacgccgttc ggacaggatg gtccctacag tgcgtacgag gcggacgacc tggtgtgcct
     9961 ggccgccggc ggcttcctgt acctgggtgg ctatccggac agcgagccca tcggcgccta
    10021 tggcgaccag gcctatctgg gcggcagcat gttcggcgcg gtggccacga tgctggccct
    10081 gacacaggcc gaagccggcg ggcagggcga gcacgtggac gtttccatgc aggaatgcat
    10141 ggtgctggcc atggagaacg ccgtgcagtt ctacgacctg gagggcatgg tgcgcaagcg
    10201 cagtgccggc atccagcgtt ttgccggcac gggcgtctac gaatgcatgg acggccatat
    10261 ctatatgatg gccggcggca tcggcgccaa ccgtttctgg ggccgcacga tcgaatggct
    10321 ggtcgccgag ggcgtgcccg atgtccagcg cctgtacggc gagcagtgga acgagaccgc
    10381 ctacctgcag tccgacgaag ccaagcggat tttcatggag gtattcggca actgggcgaa
    10441 gaccaagacc aagggctatc tctaccggga aggccagaac cggcatgtgc ccgcggcggc
    10501 gatcaatacg gtggaggact tggttgccag ccctcagctc aatgcgcgcg ggcatttcgt
    10561 gccgctggcg caaccgggct ggcccaagcc gggactgatg cccggcgcgc cgtaccagct
    10621 ggcgcgcacg ccctgggcga tcgcatcgcc cgcgccgctg ccgggccagc acaatgccga
    10681 actctatggc gaactgggcc tgtcgtccga cgaccgcgcc cggcttgccg gccggggagt
    10741 gctgtgatgc gcaaattgcc gctttccggt atccgcgtga ccgacttcac ctggataggc
    10801 gccggctcct atacaaccaa gatactggcc gatgcgggcg cggacgtcat caagatcgaa
    10861 agcgcggatc ggatcgattc gctgcggctg gccgcacctt acaaggatgg aatcaagggc
    10921 gagaaccgca gcggctattt tgccgacagg aactccagca agcgcagcct gacgctgaac
    10981 atgaaacatc cgcgcgcgct ggagctggtg acggcgctga tccggcagag cgacatcatc
    11041 gcgaacaatt tcacgccggg agtcatggac aagttcggcc tgggctacga cgccgtgcgc
    11101 ggcatcaagc ccgacatcat ctacctggcc atgtcgatgc aaggcggtac cgggccagag
    11161 agccactacc tgggctatgg ggccaccatg gccgcggtca cgggcatcca gcagctgacc
    11221 gggctgcccg gccgcgagcc ggcgggcacg ggcaccaact atcccgacca tattcccaac
    11281 ccgtgccatg cggctttcgt cctgctcgcc gcattgcgcc atcgccgccg cacgggccag
    11341 gggcagcgca tcgatatggc gcagacggag cctacggtcg ccctgctggg accggccgtg
    11401 ctggattaca ccgtcaacgg gtgcgtcccc cagcctcgcg gcaaccgcca tcccggcgcg
    11461 gcgccgcatg gcgtgtatcc ctgtgccggc gaggaccgct gggtggcggt atcggtcatg
    11521 aacgatgcgc aatggcaggc cttgtacaag gcattgggcg atccggactg gatggccgac
    11581 cccgcgctgt tgccggcggc ggagcgcgtc gcccgggccg atgtgctcga tgcgcatctg
    11641 gcccgcgaaa ccgcgcgctt cgaggcggac cgcttggtac ggctgctgca ggcccaccag
    11701 gttcccgcgg ccctggccag cacagcggcg gacatcatcg aggacccgca actggcgcat
    11761 cggcgccatt ggctcacgct tgatcatccg gagatgggcc catccctgta caacgcccag
    11821 ccttttcgct atgcgcgcgg cgcggccgcg gacgcccgtc ccgcgccttt gctgggccag
    11881 catacacgcg atgtctgcca tgaattgctg gggctgtccg acggcgagat cgaacggctc
    11941 acccgggaag gcgcgctcaa gtaaggccta gaaatagccc acctatatag ccccaatacg
    12001 gggggaacca tgcgtatagc gatcctcgac gactatcaga acctggccct gcagctggcc
    12061 gactggagcc cggtgcgggc ccgcggcgac atcgtggtct tcgaccggca cttggccgag
    12121 gaagaggctg cggccgcgct gcgggacttc gacgtcctgt gcgtgttgcg cgagcgcatg
    12181 ccattgccgg gttccttgct ggagcgcctg ccgcggctga agttcatcgc cgccaccggg
    12241 ccctacaacc ggacgctgga cctgcgcgcc gcggaccgcc tgggcattac ggtatcggcc
    12301 acgccacgag ccgccgccgg gcagtttgcc accgccgaac tggcctgggc cctgctgctg
    12361 gccctggcgc ggaatattcc tctggaggcg gccaatatgc gcgtcggcgg atggcagaag
    12421 accatgggcc tgtcgctgtc ggagcgcacg ttgggcctgg tcgggctggg gcggctgggc
    12481 agccatatgg cgcccatcgc ccgggcgttc ggcatgcgcg tgatcgcctg gagccagaac
    12541 ctgaccgccg gggcagccga ggccctgggg gcgcagcggg tcgaaaaaga cgaattgttc
    12601 gcgcgcagcg atttcgtcag cctgcacctg gtgctcggcg aacgcacgcg ccatatcgtc
    12661 ggccgccgcg agctggggct gatgaagccc gaggcctatc tgatcaatac cgcgcgcggc
    12721 gcgctggtcg agcgcgaggc gctgatcgat gccttgcggg cgcgccgcat cgccggcgcc
    12781 ggcctcgata ccttcgacat cgagccgctg ccggctgatg atcccctgcg cgcgctggat
    12841 aacgtcatcc tgacgccgca cctgggctac acggtcgaag aactgctcgg gatgttctat
    12901 gcgagcacgg tcgagaacgt gctggcgtat ctggacggca agccgctgcg cgtactgaac
    12961 gaggcctcgc cgcggctgac gggacaggtc gcggtgtagc cagccggcca aaaaaagcaa
    13021 aggaggcagt accgacatga atgagcagcg acgcagattc agccagacgg cgcttgccct
    13081 ggcctcgacc gccgtgcttg ccccgctcta tgcgtccgat gagccggcca gtaaattccc
    13141 atcgcattcg atgtacctga tgattccgtt cgaaccgggc ggcggcaccg acatcgtggg
    13201 ccgtgccatc gcgcagaaaa catccgaggc gctgggcgag gcgatggtgg tcgagaaccg
    13261 gggcggggcc aacggcacga taggcgcgag cgtggtggcc cgctcggaac cggatggcta
    13321 taccttgtgc atgttcacgg ccagccattc ggtcaacgtg acattgcagg gacgacagca
    13381 gccctatgac ttgctgcggg attttgcgcc gctcatcgag atcgcggtgc agccctatat
    13441 cctggtggtg cgacccacct tgccggtgcg gtccgtcggt gagttgatcg ccctggcgaa
    13501 gtccaggccg ggcggcctga cattcgggtc ttccggggtg ggtgggctgc tgcatctgtc
    13561 ggccgagctg ctgtcgaccc tggccggcat caagctgacc cacgtcccgt acaaaggcgg
    13621 gtcgcaggcc atgctggacg tgatggcggg caacgtcgac atgatgttca ccagcctgaa
    13681 ccagtccaag agctatatcg cctcgggcca gttgcgcctg ctggcagtga cctcgcccaa
    13741 gcgttcgccg gcggcgccgg acatccccac catgcaggaa gccggcgtgc ccggctacga
    13801 cgtgcagtca tggtacggct ttgccgttcc cgccaaggtg ccggcgccca tcatcgacaa
    13861 actcaaccgc acgatcaaca aagtgctggc gctgccggaa gtgcagcggc aaatggccat
    13921 cgacggttcg acgccggtgg gcgggtcgcc ggccgatttc ggccgcttcc tgagcgccga
    13981 agtggtcaag tggcgcgacc tgatccatca ggttggaatt cccgtgggtt gatgtccatg
    14041 atgtgaacta tttttctatc gtcgtcgata aaaatgcatg attcacgtgg ttttgaatga
    14101 gatttcgcga ggcatcgagc atgccggaat atggactatc aaaaaaatgg ataaaacaat
    14161 gaacacagga gggactgcgc atggacggcg tcgatagcaa ggcggcaacg agcccgcccg
    14221 gcgcgctgga gggcgtccgg gtgctggacc tgtccgggcc gctgggcaac tattgcggca
    14281 agctgttcgc ggaaatgggc gcggacgtca tcctggtcga accgaccggc ggcacccgcg
    14341 ctcgtttcga accgccgttc ctggacgctt cgccaggctt ggagcgcagc ctttccttcg
    14401 cctactacaa caccagcaag cgcggcgtca cgctcgacct ggattgcacg tccgggcaag
    14461 ggctgctgcg cagactcgcg gcccgcgccg acatcgtcat cgaaaccgaa aagccgggca
    14521 agatgcgcga gcgcggcttg gcctacgacg acctgatccg ggtgaacccg tcgttggtgt
    14581 atacgagcat cacgcctttc gggcagtccg ggccgtatgc gcaatacgcg gccgaagaca
    14641 tcaacggact ggcgatgggc ggactgctgt atctgggcgg ctacgccgat acgcctccga
    14701 tgcgcatgca tggcaaccag ggcattctat gcgccaatat gtatgcctcg gtggcgacca
    14761 tgctggcgct attgcaccag gagaagtccg gccaaggcca gcaggtggat gtgtcgatgc
    14821 aggaatgcat cgtgatggcg ctcgagaact ccgtgcagtt cttcgacctg gagcagacgg
    14881 tgcgcaagcg tcacgcgggc gagcagcgct tcgcgggcac cggcgtgttc gcatgccagg
    14941 acggggacat ctatctgatg gcgggcggca tcggcgccaa taaattctgg gatcggacca
    15001 tcgactggtt cgccgaggaa aaaatggccg gcgtcgaacg gctgcgccag gaagagtggg
    15061 cccggatcga atacctgcga tcggacgagg ccaagcatat cttcgccgaa ctgttcatcc
    15121 catgggccag gcagcgcagc aaagactatc tctatcggga aggacagcgc cggcgcattc
    15181 ccatcgccac catctccgaa acctcggacc tgctcggcag tccgcaactg gctttccggg
    15241 agtatttcac caaggttgcg catcccttgc gcgaggagcc gctgctgatg cctggcgcgc
    15301 cgtataaatt gtcggagacg ccctggcgca tccagcggcc ggcccccatg ctgggcgagc
    15361 acaatgcgga aatctacagc gagatcggcg tcgactcgtc ggaactggag cagttgttcg
    15421 ctgcgggggt gatatgaaag acgaaaaaat cctggaaggc atccggataa cggacttttg
    15481 ctggctgggc gcgggcgcct atgcgacgaa aaccatggcc gacttcggcg ccgacgtgat
    15541 caagatcgag aacgcctcgc gcatcgattc gctgcgcatg gccgcgccct acaaggacaa
    15601 gcagccgggc gtcaaccgca gcggctattt cgccgaccgc aacaccaaca agcgcagcct
    15661 gaccctggat atgaagaatc cccgggccat ggccctgatc aggcggctga tcgaacagag
    15721 cgatgtggtg tccaataact tcacgccgag cgtcatggaa aaattcggcc tgagctacca
    15781 gcaggtgcgg gagatcaagc ccgacatcat ctatctggcc atgtccatgc agggcacgca
    15841 aggtccggag cgcgattacc tgggctatgg cgcgagcatg gtcgcgctga ccggcctgca
    15901 gcacctgacc ggcctgcccg agcgcgaggc ggccgggccg ggaacgaatt atcccgacca
    15961 tgtccccaat ccctgccatg ccgcctttgc tttgctggcg gccatacgcc accgccgccg
    16021 cacgggccag ggccagttca tcgacctggc ccagatcgag ccgacgatcg cgctgctggg
    16081 gcccacggtg ctggacctga cggtcaacgg acgcaaccac aaccgcgacg gcaacctgca
    16141 cccctatgcg gcgccgcacg gcgcgtatcc cggggcaggg caagaccgct ggctgacgat
    16201 cgcggtgatg aacgatgcgc aatgggatgc cctggtgcag gcgatggact tgcccgcgtg
    16261 ggcgcatgac gaacgatggc gggacggcgc ctaccggcat cgcaaccgcg ccgagctcga
    16321 caccttgctg gcgggggaga ccgccggctg ggtcgcggag gaattgatgg ccgtcctgca
    16381 ggcccggggc gtgcccgcgg gcgtggtgca gaacaccgaa gacgtggtca cgcacgatcc
    16441 tcagttgcgt tcacgcggcc attgggtgcg gctgcggcat gcggagatgg gagaaaccct
    16501 ctataacgcg ccacccttcc gcttttcgga gatatcgact agcctgcggc gacccgcgcc
    16561 gctgctgggc gagcacactc gcgaggtctg cgccgatctg ctgggattga gcgacggcga
    16621 aatcgacgag ttgatggccc aaggggtatt gcaatagcgc agcgctcaat cgattcgaac
    16681 agaggttccc atgaatctgg agcaagcccg ccgtttcatc gccgacaccg aacaggcggg
    16741 cgacgtcctg cggttcccgt gccatggcgg gcaggccgta tggcatcgtt tcggccaggg
    16801 acgtcctttg gtcatgctgc acggcgggca tggcagctgg ttgcactgga tccgaaacat
    16861 tcaggccctg tcgcgcgcgc acgaggtctg gctgcccgat atgcctgggt tcggcggctc
    16921 cgccgagatt ccggtgcatg gcatggacgg cctggtccag gccatgctgg ccatgctcga
    16981 tgaggcgttc gcggatcgcg agatcgacct ggcggggttt tcgttcggcg gtctggtcgc
    17041 ggccaacatc gccgccgccc gccccggacg ggtacgcaag ctggccttgc tgggttcggc
    17101 ggggcacggc ggcccgcgcc gccagacggt ggacctggtc aactggcggc acagcacctc
    17161 gcaagaggcg ctgcacgcgg tcatgcgcgg caacctggcg gcgcacatgc tggccgaccc
    17221 ggcagccata gacggcctgg ccctgaccgc gcacatccgc tcctgcgagc aagtccagtt
    17281 ccgcagcaaa gggatttctc gcacctggaa gctggacgac gccttgcggc gcgtccaggc
    17341 gcccgtcctg atgatgtggg gcgagcacga cgtgacggcc gaccccgcgc tgatcttgcc
    17401 cgggttgacc gagggccatg ccctgcggga aggcctgatc ctgccggggg gcggccattg
    17461 gatacagttc gagtcggccg atgccgtcga cgcgaggctg gaagcctggt tcgccggcta
    17521 agcacggaga cggcgccgaa ggggccgcgc ggacctagcc gtccagcgcg ctctggatat
    17581 tgctggcggc ttcgatcaga taagacgcca ccagtttggc gcggtcgccg gacagcctgc
    17641 tggccagggc cgacacgctg atcgcaccca ccggagtatg gtcgcgcttg cagatggcga
    17701 cgccgatgga gctgacgccg ggcacttcca gcaagtcgtt ctgcacatat ccctggctgc
    17761 gggcggtcgc gatgcgggca cgcagtttct tctcggcgta gttcgggtat ttttccctga
    17821 cgtaggcttg gttgcccttg agtattttgt cgacctccgc gtcgggcaag gcgcttagca
    17881 gggcgattgc gccgccgccc acgttcagcg gccgccggcg gccgatgtcg agcacgaaga
    17941 ccttcacggg aaacgcgcct tcgacgcgat ccaggcagac gccgtccagg ccggaacgca
    18001 cggtcaggaa caccgtgtcg cccgtgcgct ccgcgatgga ctgcagatag gggtggcaga
    18061 tgtcgcgcag ggcggtttgc ggggcggccg agccgcccat ttcgtacatc agccggccca
    18121 ggaaataacg ccggctcttg gcgtcctggc gcaccatgtt ttccgagatc aggccttgca
    18181 gcaggcgata ggccgtgggc cgttccagac ccgccgcggt gtacagatcg accagccgca
    18241 tgcccgtccg gttgttcgat gcgatcaggc gcagcagcga aacggcgcgt ttgatggcct
    18301 gggtgccgga ggcggattcc ggcgtgggct tggcgggcat gggatgggcg ggggcagggc
    18361 gagggagcag agcggtaatg atagcctgtc cgcccgtcca ggcccggggt gccggccgcc
    18421 tattggccgc gcaagcgaaa ccgcgggatg gtgacttcgg gcgtgacggg atcgaagacg
    18481 acttcgagcg catcgccgac cttcacggca tcgttcggga ccccgacgac attgctgtac
    18541 atgcgcggcc cttcttccaa ttggaccagc gccacgttgt agggcacgtc cccggcgaag
    18601 gccttgttgt agacctggtg gaagacgatg taggagaaca ccgtcgcgcg tccggaaaga
    18661 tcatgccaat gaaagccgga atccaggcag cgcgggcaga ccgtgttgat cgggaagcgg
    18721 atgtggccgc acgcgtcgca gcgctgcatg cgcagcttgc cttccttgca gccttcccag
    18781 aaggggctgt tgtctttgtt cagcgcgggc aggggcttga ggtattcgct catggttcaa
    18841 ccttttctca gaatggtggc gttggcgcag ttcagggcct ggccgcgtcc ggtgaccagg
    18901 cagacttccg cgcccgcgac ctggcgcggc ccgcattctc cacgcatctg ccgcacgccc
    18961 tcgacgatgt gcagccagcc ttccatatac gattccgaca gattgccgcc cgcggtattg
    19021 atgggcagtt cgccgccgag ctggatgcgt ccgccttcga cgaaggggcc cgcttcgccg
    19081 ggcttgcaga agccggcgtg ctcgaaccag accagcacgc tgctggtgaa gttgtcgtag
    19141 agctggcaga cgtcgatgtc ccggggcccc acgccggcca tggcgtaggc atcgttgacg
    19201 gccagcttct ggtgcggcac gtaccaccag tcttcccgct ccatgttctc ggtcgtatag
    19261 ccctggccca ttcccgcgat cgtgaccagc ggctgcttca ggtccttggc gcgctcggcc
    19321 gtggtgacga tcacgcagac cgcgccatcg gagatcaggc agcagtccag cagatgcagg
    19381 ggctcgacga tccagcgcga gttctggtga tcgtccaggg tgatgggggt gcgcatgatg
    19441 gcgttcgggt tcatcgaggc gtggcggcgg caggccaccg cgatttcgcc cagctgccgg
    19501 ctggtggtgc cgtatagcgc catgtgccgc gacgcggtga tggcgctgtt ggcgcaggcg
    19561 gccatgaagc cccagatgcc ccaggagtcg ccccagcccg aggcccggtt gaatttgttg
    19621 ccgcccgtgg ccgccgtgtc gccgaatacg caggcgacat agttggcgaa gcccgcgttg
    19681 accgccagcg ccgcttcgtg cagcagggcg cccgccgtgc cgccgcccat gttcttgctg
    19741 ccggtgtagc gcgggttgat gcccagcgcc tcgcccaggc gcaggtagtt caacgccgct
    19801 tccggggagg tggtgcccgg catggtcagc agcccgtcga tctgttcttt cttcaggccg
    19861 ctgtcgtcca gcgcgcgctt gacggcggcg atgccgagcg tgatgggcgt ttcttccgga
    19921 aacttgcctt gcgggctggc gccgacgccg gcgatggcgg ttcgttctcg taatggatgc
    19981 atggtgggtc gtgggtggtt gaacgggaat gccgtcgatg cggccgaaga cgctgatccg
    20041 ggccggcggc ccggatcagc gccccttgaa gcggggcttg cgcttttccg cataggcctt
    20101 gcggccttcg atgcggtctt cgctgttgta gagcaggccg aacagggcgc gttcggcatc
    20161 catggcatag ggcagcggca tgtcctccgc ctgccgcacc atgcgcttga ccgcgcgcac
    20221 cgaaagcggc gcgttcgata cgatggtgcg ggcgatgtcg tgggccgcgg caagcagttc
    20281 ggcctgcgcg acgaccttgc tgaccaggcc gatgcggtgg gcttcggccg cgtc
//
LOCUS       DASWPO010000323        22019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2667586, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000323 DASWPO010000000
VERSION     DASWPO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 22019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22019
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2667586"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VGC69_17035"
     CDS             <1..491
                     /locus_tag="VGC69_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2968 domain-containing protein"
                     /protein_id="HEY0297038.1"
                     /translation="LFKPDDLTYYVALFQQRDFWQVVKTKSSQQAEATYRLFASRTAE
                     LAQIDFERIRLQAENAKAEEQLNARLARLNTLQADQALRVQQDQQVADRQTQLRAETD
                     RLGQQQQDVRDQLRDLQRQIEALQADQNRVPGVNNSRPAPKKAAKTSYRKKKAVRDTA
                     SE"
     gene            579..1037
                     /locus_tag="VGC69_17040"
     CDS             579..1037
                     /locus_tag="VGC69_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY0297039.1"
                     /translation="MALKVFDLQCEHGHVFEGWFASHDDYDAQRARGLVACPMCDSKE
                     ITKRLSAPHLNVSHLHAPEPAAPAAPASGSEAAAATLQARVMRQVRELIRSADNVGPR
                     FAEEARRIHEGEADNRPIRGTATQEERTELAEDGIDVMALPAFLDDGQLQ"
     gene            complement(1134..1688)
                     /gene="efp"
                     /locus_tag="VGC69_17045"
     CDS             complement(1134..1688)
                     /gene="efp"
                     /locus_tag="VGC69_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0297040.1"
                     /translation="MKTAQELRVGNVIMVGKDPLVVQKAEYNKSGRNAAVVKLKFKNL
                     LNGSNSESVYKADEKFDVVLLERKECTYSYFGDPMYVFMDAEYNQYEIEAESMGDALN
                     YIEEGMPVEVVFYEGRAISVELPTILVREIIYTEPAVRGDTSGKVLKPAKINTGFELP
                     VPLFCAIGDKIEIDTRTNEYRSRV"
     gene            complement(1825..2988)
                     /gene="earP"
                     /locus_tag="VGC69_17050"
     CDS             complement(1825..2988)
                     /gene="earP"
                     /locus_tag="VGC69_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEY0297041.1"
                     /translation="MRADLFCRVVDNYGDIGVCWRLARRLAHGLGWQLRLWVDDLQAF
                     ARIQPGIVPTAARQQYAGVEIVHWQDTPPAPLPQPGDVVIEAFACDPPAAFVQAMRET
                     RPIWVNLEYLSAEDWVPSYHALSSQRADGLVKYFFFPGFTPDTGGLIREPGLTAERDA
                     LQSSRAAQTDFLRGLGLDAALLETHRQGARLVSLFCYPQAPAGILAQTLRQQPGPTLL
                     LVPEGVAPGLDAQAGDGLRIVRIPFVTQPDFDRLLWCADINFVRGEDSFVRAAWAARP
                     LVWQIYPQDENVHLDKLEAWLARYPAPGAARDLARAWNWESLGQDGISHRPEARADLP
                     QPPALRDALAAALAEPAWSAWRAAARAWDAEQASGPDLADKLAIFCAKKLKTR"
     gene            complement(3075..4277)
                     /locus_tag="VGC69_17055"
     CDS             complement(3075..4277)
                     /locus_tag="VGC69_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HEY0297042.1"
                     /translation="MARTRSQSTPRLTRDASRLVTLAQALNRSGSRVEDQFWESQIGE
                     AIAKLLRQGQDGPLDAALDHLSQQDSGAYEVLIEQAETQSESIQVEKNGVRHDVLLLV
                     APIVAWTRYAIPTGPIPASAQQTLLAQLHGHVLAQDTRVELMPHLLSLDQMPRTFSET
                     WHWLHRLAARALGAETIKPALDSEVETANMLADTRYLVAAVAVPQGQALFRWQERPED
                     AASGRDACLEQWVAQAQPTIAALLPGCGIECLLPDAYYVSNREADRRVRPLSLRAAVG
                     WLEGAVNLPPAQLRAIVAGCGENHPDEYRIGFTPQHSNEVYYGCVWPLYGREDDEPAD
                     DSQPDTIDEIAALLKEYGVGEVRRIPGLLQPEFCEDCGAPYFPNPLGELVHAELPEDA
                     ASTPAHFH"
     gene            4558..5346
                     /locus_tag="VGC69_17060"
     CDS             4558..5346
                     /locus_tag="VGC69_17060"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY0297043.1"
                     /translation="MRWPVLFVSHGSPMLAVEPGLTGPVLADWSQSLPEKPKGILVVS
                     PHWMTRGLAVSTREQQVAWHDFGGFPDALYHLQYTAPGSPALAARVRQVLADAGLSSA
                     DDPRRPLDHGAWVPLRYLYPDAGIPVVQLSLDAGRDAAAQYELGRALAPLRDEGILVI
                     GSGSLTHNLREYGGAHDAAPLDYVPGFQRWYAERLQGNRIDELLQWEFEAPNAHRAHP
                     SDEHLMPLYVALGAGNGDTAHRLNDEVAYGALAMDAYAFGEPKN"
     gene            complement(5558..8272)
                     /gene="acnA"
                     /locus_tag="VGC69_17065"
     CDS             complement(5558..8272)
                     /gene="acnA"
                     /locus_tag="VGC69_17065"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY0297044.1"
                     /translation="MPHNTLDTLKTFKIGKQTGQYYSLPALGRALGVDVQKLPISIRI
                     VLESVLRNCDGKKVTEEHVRQLAHWGANDKREDEIPFVVARVVLQDFTGVPLLADIAA
                     MRSVAEKMGKDPKRIEPLVPVDLVVDHSVMIDYFGTKGALDLNMKLEFQRNKERYQFM
                     KWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHHAKGSAKGDIYYPDSLVGTDSHTTM
                     INGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVELKGKLRGGVTATDLVLTITEML
                     RKEKVVGKFVEFCGPGTASLSVTDRATIGNMAPEYGATMGFFPVDERTIDYFKGTGRT
                     EAEIAAFEGYFKAQKMFGVPKATDIEYTKLLTLDLSTVAPSLAGPKRPQDRIEIGNVK
                     NTFADLYSKPVAENGFNQPAEKLAETFVTSAGTQVKNGDVLIAAITSCTNTSNPSVML
                     AAGLLAKKAVEAGLTVPRRIKTSLAPGSRVVTEYLDKTGLLPYLDKLGFNVAAYGCTT
                     CIGNAGDLTADLNEAIIGNDLVCAAVLSGNRNFEARIHPNIKANFLASPPLVVAYALA
                     GTMLKDLMTEPVGKGKKGDVWLGDIWPTTEEVDALLKFALNPNVFRDNYGQVKTKPGK
                     LWENIKGVKGETYDWPDSTYIAEPPFFEGFDMTPGAMPTVKGARALGIFGDSVTTDHI
                     SPAGSIKETSPAGKWLKEHGVLKADFNSYGSRRGNHEIMMRGTFANVRIKNLMIPPTA
                     DGDRVEGGETLFQPGGEQMSIYDAAMKYVAQGTPTVVFGGEEYGTGSSRDWAAKGTQL
                     LGVKAVITRSFERIHRNNLVGMGVLPLQFKGSDSVESLKITGRETFDVSGLENGIKPM
                     QDVTLTIHREDGSKQDVTVLLRIDTPIEVDYYNHGGILPFVLRQLLAA"
     gene            complement(8342..8944)
                     /locus_tag="VGC69_17070"
     CDS             complement(8342..8944)
                     /locus_tag="VGC69_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297045.1"
                     /translation="MRGLLLLAPLLLLAGCATSSRPDNPENLCAIFREKPDWHDAAVK
                     AHDKWGIPVPVPIAMMYQESSFRADALPPRYFFLGIIPWGRVSSAYGYAQAKDDTWAD
                     YKHEAGGWFSSRDNFADSMDFMGWYMTKTHRLTGVALNDAYGQYLSYHEGWTGYRSRS
                     YDNKAWLIRVARQVQARADRFTSQYRNCPDKEGSGLFGLF"
     gene            9119..10081
                     /locus_tag="VGC69_17075"
     CDS             9119..10081
                     /locus_tag="VGC69_17075"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY0297046.1"
                     /translation="MTVHHLAASPETIHWGHFSAALKPVLVVDSGATVTIDSVNGGPG
                     DLAEIGVELLPEHRRIHEECKPMLGGHIVTGPVGVRGAAPGDVLEVRVLDVKLRQPWG
                     WNVQRPLRGTLPEDFPAKRIMPISLDLQRMTADLPFGPSVPLAPFFGIMAVAPRPEYG
                     AVSTVEPREFGGNIDNKELGKGSTIFFPVQAPLALFSAGDGHAVQGDGEVCLTAIETA
                     LTGTFQLILHKKVPLALPVGITDTHVITMGFHEDLDDAAKIALREMIALLSRTQGWSK
                     QDAYTFCSLACDLRVTQLVDGNKGIHAMVARSLIKDVDVASLSS"
     gene            complement(10227..10499)
                     /locus_tag="VGC69_17080"
     CDS             complement(10227..10499)
                     /locus_tag="VGC69_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY0297047.1"
                     /translation="MARTVNCVKLKREAEGLDYPPYPGELGARIWREISKEAWDEWKQ
                     VQTRLVNENRLNLADARARKYLQQQMERFLFEDGSVEAQGFVPPSA"
     gene            complement(10605..11966)
                     /gene="argA"
                     /locus_tag="VGC69_17085"
     CDS             complement(10605..11966)
                     /gene="argA"
                     /locus_tag="VGC69_17085"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY0297048.1"
                     /translation="MSTAQEPDTYSALEAAESAPAQFVRWFREVAPYVHAFRGRIFVV
                     AFGGELVRDGALNALVQDLSLLSSLGIRLVLVHGSRPQVNEQLRLKGYAQQFGRGLEP
                     TDAPALECAKEAAGEIRLDIEAAFSQGLPNTPMSGSHIRVVSGNFVTARPAGVIDGVD
                     YHHTGLVRKIDTDSIKFAIEHGSAVVLLSPLGFSPTGDAFNLAMEDLATSVAVALRAE
                     KLIFLSSTPGVLNDDGTVDTELARVDADALLEHGDLDEDTTFYLRSASLAVKRGVARA
                     HLVPYAQDGSVLLEIFTHDGVGTMVVEDTLDDLRAATIDDVGAILSLIEPLEADGTLV
                     PRPRSVIERDVEHFTVLEHDGVIYGCAALHPYQEEQMAEMACLIVNPEWQGSGEGEIL
                     LRHMEARARTLGMKRLFVLTTRTSHWFMKRGFVPASVADLPLAKQQHYNRSRNSLVFI
                     KKL"
     gene            12137..16042
                     /gene="hrpA"
                     /locus_tag="VGC69_17090"
     CDS             12137..16042
                     /gene="hrpA"
                     /locus_tag="VGC69_17090"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEY0297049.1"
                     /translation="MSEVSRPAGRSPERDQGRQAPRPERPIPVIAYPEDLPVSARRQE
                     IARAIAAHQVVIVSGETGSGKTTQLPKICLELGRGRRRLIGHTQPRRLAATSVARRIA
                     EELDTPLGEVVGYQVRFNDHTGPNASVKLMTDGILLAESQRDPLLKRYDTVIIDEAHE
                     RSLNIDFLLGYLKQLLPRRPDLKLIITSATIDAERFATHFASESGKPAPVIEVSGRLY
                     PVEVRYRPVLQEQPDDAAPSGKGNRDRLAGDEERDLIDAIVDAVDECARHGPGDVLVF
                     LPGEREIRESAEALRKHHPAGTEILPLYARLSQAEQEQIFRPRGNARRVVLSTNVAET
                     SLTVPGIRFVVDSGLARIKRYSWRNKVEQLRIESISRASANQRAGRCGRIGPGLCIRL
                     YDEADFNARAPFTDPEVLRSSLASVILRMKSLGLDDIERFPFVEAPPGRAVADGYHLL
                     LELGAIALAQAEDKGDEATRTGAAYELTNTGRELAKLPVDPRIGRMVLAAREQQCLAE
                     MLIIASALSVQDPRDRPMAEREAAEAAHAKFNDDKSEFVSFLKLWRWYGEQVQHKPSQ
                     RRLVGLLRQNFLSPVRLREWHDVHTQLAALVGELGWRLNQTDATYEQLHLALLSGLLG
                     NIGFKSEEGGNPGGNYQGAREIRFHIHPSSTLQKKAGRWIMAAELIETTRLYARCVAR
                     IEPTWLERVAGHLLRHNWSDPRWEKKAGQVVANERATLYGLQIYHGRRVHYGRINPQH
                     ARELFIREALVPGEIDTKLPFVAHNRRLIASIEKLEHQTRRPDILVDDELIHAFYDQQ
                     LPAGISQTATLEKWVHGLDKAAADKLLLTREALMRHEAAGVTTEVFPKKVEWQGVSMA
                     LDYHFEPGSSKDGVTLSVPLFALNQIDAQRCEWLVPGMLKEKVHLLLKSLPQKLRRHC
                     VPLPDYSAGFYDRWFDKLGDPQQGLVEAIIADMWDQVQVRPVLGDFKLETLPAHLFMN
                     FRVVDEHGRMLAAGRNLAQLKAELGRQAQANFQQLASRDTEVAQALAHEKLTSWSFGP
                     LPEVMEIKRGGQSVIGYPALVDKGAHDDLDVFDDPDEARRHHRAGLLKLFRLGLREQV
                     KFLEKNLADLTKMSMFYLTLGTQEELRDQIIDCALAQACLAEPWPANEQQFEARRNEG
                     KGRLGLLAQEVARLAGTILAEYAALQRKLQQAKPHAAAHADLQQQLGALMPKWFIREI
                     PYAQLAHYPRYLKAAVGRIDKLRADPGRDAKLMGEMAPLLTNYQRARSALKGAPDPAL
                     DEFRWLLEELRVALFAQELRTPMPVSVKRLMKAWEALRR"
     gene            complement(16094..16345)
                     /locus_tag="VGC69_17095"
     CDS             complement(16094..16345)
                     /locus_tag="VGC69_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297050.1"
                     /translation="MKNLRGKASFDDFLAEYGYLDPATEAARARAIVWQLAQGISLAG
                     LSETGTIKGLSQILARSATLALGMAEEELVRLLKNHSAH"
     gene            complement(16401..17390)
                     /locus_tag="VGC69_17100"
     CDS             complement(16401..17390)
                     /locus_tag="VGC69_17100"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0297051.1"
                     /translation="MPISSLMGTYRLILAIFVLLSHQGVSIFGLNPGITAVISFLLLS
                     GFVMTALIQKNYFAAGRILPFYLDRAMRLLPQYLAYLAATLVLILAARPVSSFVQGFD
                     FSLSKILLNVLILPLGLYMYGMENTMLIPQAWSLGLETMFYLAIPLLLVWRLRIAAFA
                     LSALVFMMAYMGLINTDVWGYRLLPGTLFIFLTGSFIYDRKIKHRRLALAVIWACAAI
                     GLAAVKQQPSLQLPNNAEVLAGLVIGIPAVCLLRNLPNSRLDTLLGNISYGVFLNHFF
                     LMWLFEALSFPATSPKRIVVLVLCSVVLSSITFNAIEAPAIRFRHLLRYRKST"
     gene            17506..17931
                     /locus_tag="VGC69_17105"
     CDS             17506..17931
                     /locus_tag="VGC69_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY0297052.1"
                     /translation="MQAMDDKQAFAERLKKALKRSHKKIETPTDLATQFSLRSNEPVT
                     PQAAQKWLSGRAIPTPDKISILAEWLDVSEKWLRHGVADEPRHSHRGRPAAMREPQAP
                     SAAEWTLLERLRQLPEARRKLVEDLVEQLSLDAEVWKRS"
     gene            18093..19475
                     /locus_tag="VGC69_17110"
     CDS             18093..19475
                     /locus_tag="VGC69_17110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0297053.1"
                     /translation="MGTQPRQIHLNAFDMNCVGHIQQGLWTHPRDQSTRYLDIDYWTS
                     LARKLEAGLFDGLFFADVLGVYDVYGASPDAAIRGAVQVPVNDPALLIPAMAAATKHL
                     GFGITCNLTYEPPYAFARRMSTLDHLTRGRIGWNIVTGYLDSAARAAGLSGQMAHDDR
                     YDLADEYMSLVYKLWEGSWEDGAVQADRASGQYADPAKVHVVRHQGPQYKVNALHLAE
                     PSPQRTPVLYQAGASTRGRLFAATHAECVFVNGQSKDGVKRLADDIRAQAIALGRAAG
                     DIKIFMGATVITGRTDAEAREKFEEYRRYASSEAALAHAAASMGIDFSKYDLDEPIRT
                     ESDGKQAIVSNVEAMSRSAGPAWTKRKLLEQMVLGSRQAPWVGSAGKIADALIAWNAE
                     AGVDGFNLSRTVVPECFDDFIELVVPVLQERGAYKTSYREGPYRQKLFGQARLPATHA
                     AAAHRRGTQA"
     gene            19479..20714
                     /locus_tag="VGC69_17115"
     CDS             19479..20714
                     /locus_tag="VGC69_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297054.1"
                     /translation="MPQQMRVRPGAGLGKTGSLIVLLLCEIGAMSVWFSSAAAVLALR
                     QAGGVSPMAEALLTSAVQAGFVAGTLASALLGLADRYDPRRLFAVCSLAAGAATFVLG
                     AIAPAGIEVYALRFLTGACMAGIYPVGMRLAATWANRDLGLLMGLLVGALTLGTASPH
                     LVSLAGGAEWHVVYRVAGCCALLAGAAINVCGIGPSMRRGASLHWAKAVLAWRDRPLR
                     LANLGYLGHMWELYAMWAWLAVFIKASFTERGLADPQASAEWLTSAAIGLGFVGAWLG
                     GLLADRHGRTAVTIVAMIVSGLCAASMGWAYGGPVWLVAALAVLWGVSVIADSAQFSA
                     SVAELSPPDAVGTLLTAQTCAGFLLTLVSIHAVPWVAAALGWPLAFGMLAVGPFLGCV
                     AMARLRADPAAVRLAGGRR"
     gene            complement(20735..21634)
                     /gene="lpxO"
                     /locus_tag="VGC69_17120"
     CDS             complement(20735..21634)
                     /gene="lpxO"
                     /locus_tag="VGC69_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="HEY0297055.1"
                     /translation="MKWFIPALWIASILFAHFRGRVRLPLSRQLLDHSVILAPVNAFM
                     VLTSRVPTTPYLTSREFPELKALDDNWQTIRDEALHMAELRHIKAAEKHDDIGFNSFF
                     KYGWKRFYLKWYDARHPSAEQLCPKTVALLKTLPSVKAAMFAELPPGGKLNPHRDPFA
                     GSLRYHLGLTTPNDDRCYIDVDGERYSWRDGQSVIFDETYIHEAYNTSDINRIILFCD
                     VVRPLKWRWADAFNQWFSRVFMSAASSPNDSGDQTGLVNKLTHAHWLIDQKRKAFKAW
                     NRTVYKATKYGLIVLVIAGFIAL"
     gene            21750..>22019
                     /locus_tag="VGC69_17125"
     CDS             21750..>22019
                     /locus_tag="VGC69_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY0297056.1"
                     /translation="MSEAAPISAPLAVQSTSESVAAPAFVHLRVHSEFSVVDGLVRIP
                     DLVKRVASLGQPAVALTDLSNLFGLIKFYKSARGAGVKPVAGCDVW"
BASE COUNT         3595 a         7101 c         7503 g         3820 t
ORIGIN      
        1 tgctcttcaa gcccgacgac ctcacctatt atgttgcgct tttccaacaa cgtgatttct
       61 ggcaggtcgt caaaaccaag tcttcccagc aggccgaggc gacttatcgc ctctttgcct
      121 cgcgcacggc tgaactggcc cagattgatt tcgagcgcat ccggctgcag gccgaaaacg
      181 ccaaggccga ggagcagctc aatgcccgcc tggcccgcct gaacaccctg caagccgacc
      241 aggccctgcg cgtgcagcaa gaccagcagg tggccgaccg ccagacccag ctgcgcgccg
      301 agaccgaccg cctgggccag cagcagcagg acgtccgcga ccagctgcgt gacctgcaac
      361 gccagatcga agccctgcag gccgaccaaa accgcgtgcc gggggtcaac aacagccgtc
      421 cggcgcccaa gaaggcggcc aagacctcct atcgcaagaa gaaggcggtc cgcgatacgg
      481 cttcggaata agggcttgca tgcgccgcgc cgcttgccgg tgcacccgga ggagcggccg
      541 gcccttgtct tttggccttt tgtgctcata tagaagccat ggcacttaaa gttttcgacc
      601 ttcaatgcga gcacggccac gttttcgagg gctggttcgc ctcgcacgac gactacgacg
      661 cccagcgcgc ccgggggctg gtcgcctgtc ccatgtgtga ctcgaaagag atcaccaagc
      721 gcctgtcggc gccgcatctg aacgtctcgc atctgcacgc gcccgaaccg gcggcgccgg
      781 cagcccccgc gtccggcagc gaggcggcgg ctgccaccct gcaggcccgc gtcatgcgcc
      841 aggtgcgcga gctgatccgc tcggccgaca atgtcggccc gcgctttgcc gaagaagccc
      901 gtcgcatcca cgaaggcgag gccgacaacc gtcccatccg cggcacggcc acgcaggaag
      961 agcgtaccga gctggccgag gacggtatcg acgtcatggc cctgccggct ttcctggacg
     1021 acgggcaact gcaataaagg gctcgccgcg caggcggcga agccggaaat gaaaaaaccc
     1081 tcggcgatcc gagggttttt tgcatccccc gcgcgaatgg cggggggggg cgattacacg
     1141 cggctgcggt attcgttggt gcgggtgtcg atttcgatct tgtcgccgat ggcgcagaag
     1201 agcggcacgg gcagttcgaa gccggtattg atcttggcgg gcttgagcac cttgcccgag
     1261 gtgtcgccgc gcacggcggg ctcggtgtag atgatttcgc gcaccaggat ggtgggcagt
     1321 tccaccgaga tggcgcggcc ttcgtagaag accacttcca cgggcatgcc ttcttcgatg
     1381 tagttcaggg cgtcgcccat gctttcggct tcgatctcgt actggttgta ctcggcgtcc
     1441 atgaagacgt acatggggtc gccgaagtac gagtaagtgc attccttgcg ttcgagcagc
     1501 accacgtcga acttttcgtc ggccttgtag accgattcgc tgttcgagcc gttgagcagg
     1561 ttcttgaact tcagcttgac cacggccgcg ttacggcccg acttgttgta ttcggccttc
     1621 tggaccacga gcggatcctt gccgaccatg atcacgttgc cgactcgcaa ttcctgagcg
     1681 gttttcatct aatcaaaact ccggggtgta agaagcgcgc gggttttcaa gcaaggctcg
     1741 gttgcgctgg cgcaatggcc cgtcgctcga aaccgacgca cagttcccgg caaagcgaaa
     1801 ggccgggcta gaaagcccgt tattctagcg tgttttgagc ttttttgcgc aaaaaatggc
     1861 cagtttgtcg gccaaatcgg ggccggacgc ttgttcggcg tcccaggcgc gggcggcggc
     1921 ccgccaggcg ctccaggccg gttcggccag tgccgccgcc agggcatcgc gcagggcggg
     1981 cggctgcggc aagtccgcgc gggcctcggg gcggtggctg atgccgtcct ggccgaggct
     2041 ttcccaattc caggcacggg ccagatcccg ggcggcgccg ggggcgggat agcgggccag
     2101 ccaggcttcg agcttgtcca gatgcacgtt ttcgtcctgg gggtagatct gccagaccag
     2161 cggccgggca gcccaggcgg cgcgcacgaa agagtcttcg ccgcgcacga agttgatgtc
     2221 ggcgcaccac agcaggcggt cgaaatccgg ctgcgtgacg aaggggatgc ggacaatgcg
     2281 caggccgtcg ccggcttgcg cgtccaggcc cggcgccacg ccctcgggca ccagcagcaa
     2341 ggtggggccg ggctgctggc gcaaggtttg cgccaggatg ccagccgggg cctgcgggta
     2401 gcagaacagg gaaaccagcc gggcgccctg gcggtgcgtc tccagcagcg cggcatccag
     2461 gcccagcccg cgcaggaaat cggtctgcgc ggcgcgcgag gactgcagcg cgtcgcgctc
     2521 ggccgtcagg cccggttcgc gtatcaggcc gcccgtgtcc ggggtaaagc cgggaaagaa
     2581 gaaatacttc accagcccgt cggcgcgctg cgaggacagg gcgtggtagc tgggcaccca
     2641 gtcttcggcg ctcaggtatt ccaggttgac ccagatgggt cgcgtctccc gcatggcctg
     2701 cacgaaggcg gccggcgggt cgcaggcaaa cgcctcgatc accacgtcgc ccggctgggg
     2761 caggggagcg ggcggggtgt cctgccagtg gacgatctcg acgccggcgt actgctggcg
     2821 cgcggccgtc gggacgatgc cgggctggat gcgggcaaag gcctgcaggt cgtccaccca
     2881 gaggcgcaac tgccagccca gcccgtgcgc caggcggcgc gccaggcgcc agcacacccc
     2941 gatatcgcca tagttgtcga cgacccggca gaacaggtcg gctcggatac tgtctccacg
     3001 ctgcgcctgc ggcttgctgc cccccggggg gcgcccttcg ccttggggag gcccggcggc
     3061 gaaggggctg gtcatcagtg gaaatgggcc ggcgtgctgg cggcgtcttc gggcagttcg
     3121 gcgtggacca gctcgcccag cggattgggg aagtagggag cgccgcaatc ctcgcagaac
     3181 tcgggctgca gcaggccggg gatgcggcgc acttcgccca cgccgtattc cttgagcagg
     3241 gcggcgattt cgtcgatggt atcgggctgg ctgtcgtcgg cgggctcgtc gtcctcgcgg
     3301 ccgtagagcg gccacacgca gccgtaatac acctcgttgc tgtgctgcgg cgtgaagccg
     3361 atgcggtatt cgtccggatg gttctcgccg cagccggcca cgatggcgcg caactgggcc
     3421 gggggcaggt tgacggcgcc ttccagccag ccgaccgccg cgcgcagcga caggggccgc
     3481 acgcggcggt cggcttcgcg attgctgacg taataggcat cgggcagcag gcactcgatg
     3541 ccgcagcccg gcagcagcgc ggcgatggtg ggctgggcct gggccaccca ttgttccagg
     3601 caggcatcgc ggccgctggc cgcgtcttcg gggcgttcct gccagcgaaa cagcgcctgg
     3661 ccctgcggca cggccacggc ggccaccaga tagcgggtgt cggccagcat attggccgtt
     3721 tccacttcgc tgtccagggc gggcttgatg gtctcggcgc ccagggcccg ggcggccagc
     3781 cggtgcagcc aatgccaggt ttcggagaag gtgcgcggca tctggtccag gctgagcagg
     3841 tggggcatca gttcgacgcg cgtgtcctgg gccagcacat ggccgtgcaa ctgggccagc
     3901 agcgtttgct gcgcggaggc cgggatggga ccggtgggga tggcgtagcg cgtccaggcc
     3961 acgatgggcg ccaccagcag cagcacgtcg tggcgcacgc cgtttttctc gacctgtatc
     4021 gactcggact gcgtttcggc ctgttcgatc agcacctcgt aggcgccgga atcctgctgc
     4081 gatagatgat cgagtgcggc gtccagcggg ccgtcctggc cctggcgcag cagtttggca
     4141 atggcctcgc cgatctggga ttcccaaaat tggtcttcca cccggctgcc cgaccggttc
     4201 agggcctggg caagcgtcac caggcgcgag gcgtcgcggg tcagtcgggg cgtgctttgg
     4261 ctgcgggttc gtgccatggg ataagtcaca gacgtcggtt caaactgcgt caagggaaac
     4321 cccatagtgt accccttgcc cacgccgtga agaggcagga ccgcctggcg cccggcaaag
     4381 cgccgggtac gccttgtttc ggatcacggc agccaggctg cccaggcgct ggccgcccgc
     4441 cggcggcctg cattggtgcg ccctgggcaa cagtgcgtgt tgcccagggc ggtttattcc
     4501 cgcggacaaa gcgcccagaa tcagggtgtc cactatttat tcgccggagt ttccgccatg
     4561 cgctggcctg tcctgttcgt ttcccacggt tcccccatgc tggccgtcga gccgggcctc
     4621 accgggcccg tcctggcgga ctggagccag tccctgccag aaaagcccaa gggcatcctg
     4681 gtcgtgtcgc cgcattggat gacccgcggc ctggcggtct ccacccgcga gcagcaagtg
     4741 gcctggcacg acttcggcgg ctttcccgac gcgctctacc acctgcaata cacggcgccg
     4801 ggctcgccgg ccctggctgc gcgcgtgcgc caggtgctgg ccgacgcggg cctgtcctcg
     4861 gccgacgacc cgcgccgccc gctcgaccat ggcgcctggg tgccgctgcg ctatctctac
     4921 ccagatgccg gcatccccgt ggtgcagctg tcgctggacg ccggccgcga tgccgcggcc
     4981 cagtacgaac tgggccgcgc cctggcgccg ctgcgcgacg agggcattct ggtgatcggt
     5041 tccggctcgc tcacgcacaa cctgcgcgaa tacggcggcg cccacgatgc cgccccgctt
     5101 gattacgtgc cgggcttcca gcgctggtat gccgagcgtc tgcaaggcaa ccgcatcgac
     5161 gaattgctgc agtgggaatt cgaggcgccc aacgcgcacc gcgcccatcc cagcgatgaa
     5221 cacctcatgc cgctctatgt ggccctgggg gccggcaatg gcgacacagc ccatcgcctg
     5281 aacgacgagg tcgcctacgg cgccctggcc atggacgcct acgccttcgg cgagccgaag
     5341 aattgagata ctggagtcag ccgtccaggc cttccctcgc agcctgttga ggcaggggaa
     5401 gccccgcagg ggccgagggc gcctgatggc gcgcccgagc cctgccgatc aggctgcgag
     5461 ggaaggcctg gacggctgac gtttcaagaa cccaaaaaaa tcggtccggt cgacgaaaga
     5521 ccgggccgat ttgctttcta gcagccgtcg cgcgcgctca agccgccagc aactgccgca
     5581 gcacgaaggg caggatcccg ccgtggttgt agtagtccac ctcgatcggc gtatcgatcc
     5641 gcagcagcac ggtgacgtcc tgcttgctgc cgtcctcgcg atggatggtc agcgtcacgt
     5701 cctgcatggg cttgatgccg ttttccaggc cggagacatc gaacgtctcc ctgcccgtga
     5761 tcttcaggct ttccacactg tcgctgccct tgaactgcag cggcagcacg cccatgccca
     5821 ccaggttgtt gcggtggatg cgctcgaagc tgcgggtgat caccgccttc acgcccagca
     5881 gctgcgtgcc cttggccgcc cagtcgcgcg acgagcccgt gccgtattcc tcgccgccga
     5941 agaccacggt cggcgtgcct tgcgccacgt acttcatggc cgcgtcgtag atcgacatct
     6001 gctcgccgcc gggctggaac agtgtctcgc cgccttcgac gcggtcgccg tcggccgtgg
     6061 gcgggatcat caggttcttg atgcgcacat tggcgaaggt gccgcgcatc atgatctcat
     6121 ggttgccgcg ccgcgagccg tagctgttga agtcggcctt gagcacgccg tgttccttga
     6181 gccacttgcc cgcgggcgag gtttccttga tggagccggc cggcgagatg tggtcggtgg
     6241 tgaccgagtc gccgaagatg cccagggcgc gggcgccctt cacggtgggc atcgcgcccg
     6301 gcgtcatgtc gaagccttcg aagaagggcg gttcggcgat gtaggtggag tcgggccagt
     6361 cgtaggtttc gcccttgacg cccttgatgt tctcccacag cttgcccggc ttggtcttga
     6421 cctggccgta gttgtcgcga aagacattgg ggttgagcgc gaacttgagc agcgcgtcga
     6481 cctcttccgt ggtcggccag atatcgccca gccagacgtc gcctttcttg cccttgccca
     6541 ccggctcggt catcaggtct ttcagcatcg tgccggccag ggcataggcc accaccagcg
     6601 gcggcgaggc caggaagttc gccttgatgt tggggtggat gcgcgcctcg aagttgcggt
     6661 tgcccgacag caccgccgcg cataccaggt cgttgccgat gatggcttcg ttcaggtcgg
     6721 ccgtcaggtc gccggcattg ccgatgcagg tagtgcagcc gtaggccgcc acgttgaagc
     6781 ccagcttgtc gagatagggc agcaggcccg tcttgtccag gtactcggtc acgacgcgcg
     6841 agccgggggc cagcgaggtc ttgatgcgcc tgggcacggt cagcccggcc tcgaccgcct
     6901 tcttggccag caggcccgcc gccagcatca cgctggggtt ggaggtgttg gtgcaggagg
     6961 tgatggcggc gatcagcacg tcgccgttct tgacctgggt gccggcgctg gtgacgaagg
     7021 tctcggccag tttctcggcg ggctggttga agccgttctc ggccaccggc ttggaataca
     7081 ggtcggcgaa ggtgttcttg acgttgccga tctcgatacg gtcctgcggg cgcttggggc
     7141 cggccagcga cggtgcgacg gtggagagat ccagcgtgag cagcttggtg tattcgatgt
     7201 ccgtggcctt gggcacgccg aacatcttct gcgccttgaa gtagccctcg aaggcggcga
     7261 tctccgcttc ggtgcggccg gtgcccttga agtagtcgat ggtgcgttcg tccacgggga
     7321 agaagcccat ggtcgcgccg tactcgggcg ccatattgcc gatggtggcg cggtcggtca
     7381 ccgagaggct ggcggtgccg gggccgcaga attccacgaa cttgcccacc accttttcct
     7441 tgcgcagcat ttcggtgatg gtcagcacca ggtcggtggc ggtgacgccg ccgcgcagct
     7501 tgcctttcag ctccacgccc accacgtcgg gggtgaggaa gtacaccggc tggcccagca
     7561 tgccggcctc ggcctcgatg ccgcccacgc cccagcccac cacgccgatg ccgttgatca
     7621 tggtggtgtg gctgtcggtg cccaccagcg aatcgggata gtagatgtca cccttggcgc
     7681 tgcccttggc atggtgcacg ccgcgcgcca ggtattccag gttgacctgg tgcacgatgc
     7741 caaagcccgg gggcaccacg ccgaaggtgt cgaaggcctg catgccccac ttcatgaact
     7801 ggtagcgctc tttgttgcgc tggaattcca gcttcatgtt caggtcgagc gcgcccttgg
     7861 tgccgaagta gtcgatcatc accgagtggt cgaccaccag gtccaccggc accagcggct
     7921 cgatgcgctt ggggtccttg cccattttct cggccaccga gcgcatggcg gcgatatcgg
     7981 ccagcagcgg cacgccggtg aagtcctgca gcaccacgcg ggccacgacg aaggggatct
     8041 cgtcctcgcg cttgtcgttg gcgccccaat gggccagttg gcgcacgtgc tcttcggtga
     8101 ccttcttgcc gtcgcaattg cgcagcaccg attccagcac gatgcgtatc gatatcggca
     8161 gcttctgcac gtccacgccc agggcgcggc ccagggctgg cagcgagtag tactggccgg
     8221 tttgcttgcc tattttgaat gtcttgagcg tgtctagcgt gttgtgcggc atggtggctc
     8281 cttgatgtga tgggttcgac gcgacggact gcaccatttt ccggtgtttt ccggtgcttg
     8341 gctagaacag cccgaacagg cccgaacctt ccttgtccgg gcagtttcga tactggctgg
     8401 tgaatcggtc cgcgcgggcc tgcacctggc gggccacccg gatcagccag gccttgttgt
     8461 cgtagctgcg gctgcggtag cccgtccagc cttcgtggta gctgaggtat tggccgtagg
     8521 cgtcgttcag ggccacgccg gtcaggcgat gggtcttggt catgtaccag cccatgaagt
     8581 ccatggagtc ggcaaagttg tcgcggctgg agaaccagcc gccggcctcg tgcttgtagt
     8641 cggcccaggt atcgtccttg gcctgggcgt agccgtaggc cgagcttaca cggccccagg
     8701 gaatgatgcc caaaaagaag tagcgcggcg gcagggcgtc ggcccggaag ctcgattcct
     8761 ggtacatcat ggcaatgggc acgggcacgg ggatgcccca cttgtcgtgc gccttgacgg
     8821 cggcgtcgtg ccagtcgggc ttttcccgga agatagcgca gaggttctcg gggttgtccg
     8881 gccgcgatga ggtggcgcag ccggccagca agagcagtgg cgccaggagc agcaatcctc
     8941 gcatgaccgt gtttcccatt cctcgagcat ctggacgatg tcggggattt taagcgcgtc
     9001 ccgccaattg tgatcaattg cgacaggggc tgtgatacct tcctccgata tgtggcggac
     9061 cggctgcgtc gcaaaggcag cggggccgta tcgtttcaac ttttattcag acaagtccat
     9121 gactgttcac cacctcgccg cctctcctga aaccatccat tggggccatt tcagcgcggc
     9181 gctcaagccc gtgctggtgg tcgattccgg cgctaccgtc accattgatt cggtcaacgg
     9241 cggcccgggc gatcttgccg aaatcggcgt ggagctgctg cccgaacacc gccgcatcca
     9301 tgaagaatgc aagccgatgc tgggcgggca tattgtgacc ggcccggtcg gcgtccgcgg
     9361 cgcggcgccg ggcgatgtgc tggaagtgcg ggtgctggac gtgaagctgc gccagccttg
     9421 ggggtggaat gtgcagcgcc cgctgcgcgg cacgctgccg gaagattttc ccgccaagcg
     9481 catcatgccg atttcgctcg acctgcagcg tatgacggcc gacctgcctt tcggcccgtc
     9541 ggtgccgctg gcgcctttct tcggcatcat ggccgtggcg cctcgccccg agtatggcgc
     9601 ggtcagcacc gtcgagccgc gcgagttcgg cggcaacatc gacaacaagg agctgggcaa
     9661 gggctcgacg atctttttcc cggtgcaggc gccgctggcc ttgttctcgg ccggcgacgg
     9721 ccatgccgtg cagggcgacg gcgaggtgtg cctgacggcc atcgaaacgg cgctgacggg
     9781 cacgttccag ttgatcctgc acaagaaggt gccgctggcg ctgccggtgg gtatcaccga
     9841 tacgcacgtg atcaccatgg gttttcacga ggatctggac gatgccgcca agatcgcctt
     9901 gcgcgagatg atcgcgttgc tgtcccgcac ccaggggtgg tcgaagcagg atgcctatac
     9961 gttctgcagc ctggcttgcg atctgcgggt gacgcagctg gtggacggca acaaggggat
    10021 ccacgcgatg gtggccaggt cgttgatcaa ggacgtggat gttgccagtt tgagttcttg
    10081 aggcgtccgt cgcgggccac ggggcgcagc ggcttcgctg agccgggctc gggcgcgcca
    10141 tcaggcgccc tcggcccgct gcgcgggctt ccccgcctca gaggcgccgc tgcgccccgt
    10201 ggcccgcgac ggactcaagg catcaattag gcggagggtg ggacgaagcc ttgcgcttcg
    10261 acgctgccgt cctcgaacag gaaccgttcc atctgctgct gcaggtactt gcgggcgcgc
    10321 gcgtcggcca ggttgaggcg gttttcgttg accaggcgcg tctgcacctg cttccactcg
    10381 tcccaggcct ctttcgagat ctcgcgccag atgcgggcgc cgagttcgcc ggggtagggc
    10441 ggatagtcca gcccctcggc ttcgcgcttg agtttcacac aattgacggt acgggccatg
    10501 atctgctgcc gatgattgtt gaagagatgg ggcgatttta gcctgcgccg gaaaacacaa
    10561 gcccctgctt cgggcagggg cttgcggcgg ggcgggctgg ttcgtcagag tttcttgatg
    10621 aacaccaggc tgttgcgcga gcggttgtag tgctgctgct tggccagcgg caggtcggcc
    10681 acgctggcgg gcacgaagcc gcgcttcatg aaccagtgcg aagtgcgggt ggtcagcacg
    10741 aacaggcgct tcatgcccag cgtgcgggcg cgggcctcca tgtggcgcag caggatctcg
    10801 ccttcgcctg aaccctgcca ttcggggttc acgatcaggc aggccatctc ggccatctgc
    10861 tcttcctggt agggatggag cgccgcgcag ccgtagatca cgccgtcgtg ctccagcacg
    10921 gtgaagtgct ccacgtcgcg ctcgatcacg ctgcgcgggc gcggcaccag ggtgccgtcg
    10981 gcttccagcg gctcgatcag gctgaggatg gcgcccacgt cgtcgatcgt ggccgcgcgc
    11041 aggtcgtcca gcgtgtcttc caccaccatg gtgcccacgc cgtcgtgggt gaagatctcc
    11101 agcagcacgc tgccatcctg ggcgtagggc accaggtggg cgcgggccac gccgcgcttg
    11161 acggccagcg aggcactgcg caggtagaag gtggtgtctt cgtccaggtc gccatgctcg
    11221 agcagggcgt cggcgtcgac gcgggccagt tcggtgtcga ccgtgccgtc atcgttgagc
    11281 acgccgggcg tgctcgacag gaagatgagc ttttcggcgc gcagcgccac ggccacgctg
    11341 gtggccaggt cttccatggc caggttgaag gcatcgcccg tgggcgagaa gcctagcggc
    11401 gagagcagca ccaccgccga gccgtgctcg atggcgaact tgatcgagtc ggtgtcgatc
    11461 ttgcggacca gcccggtgtg gtgatagtcc acgccgtcga tcacgccggc ggggcgggcc
    11521 gtgacgaaat tgcccgacac cacgcggatg tgcgagcccg acatgggggt attgggcagc
    11581 ccctggctga aggcggcctc gatgtccagg cggatctcgc cggcggcttc cttggcgcat
    11641 tccagcgcgg gggcgtcggt gggttccagg ccgcggccga actgctgggc gtagcccttc
    11701 aggcgcagct gttcattgac ctgcggacgc gatccgtgca ccagcaccag gcggatgccc
    11761 agcgacgaca gcagggagag atcctgcacc agggcattga gcgcgccgtc gcgcaccagc
    11821 tcgccaccga aagccaccac gaaaatcctg ccccggaagg catggacata aggcgccacc
    11881 tcgcgaaacc atcggacgaa ctgggcgggg gcggattcag cggcttctag ggcggagtag
    11941 gtgtcgggtt cctgggcggt ggacatggtg gcgcgaacgc ggtcggggca agtggacaaa
    12001 ggcggggccg agccccgccg ccggcgccgc ggtcagcggc gctgcgcgga attataggac
    12061 cgggaaccac ccgggcagac gggttcgtgt ccgggtgaaa cctataatgt tggtcaatac
    12121 gcacatcatc ccttccatgt ccgaagtctc acgccccgcc gggcgctcgc cggaacgcga
    12181 tcagggccgc caggccccgc gcccggagcg gcccattccc gtcatcgcct atccggaaga
    12241 cctgcccgtc agcgcaaggc gccaggagat cgcgcgcgcc atcgccgcgc atcaggtggt
    12301 gatcgtcagc ggcgagaccg gctcgggcaa gaccacgcag ctgcccaaga tctgcctgga
    12361 gctgggccgg gggcgccgcc gcctgatcgg ccacacccag ccgcgccgcc tggccgcgac
    12421 ttccgtcgcc aggcgcatcg ccgaagagct cgacacgccc ctgggcgagg tggtgggcta
    12481 ccaggtgcgc ttcaacgacc acaccggccc gaacgcgtcg gtcaagctga tgaccgacgg
    12541 catcctgctg gccgagtcgc agcgcgaccc gctgctcaag cgctacgaca ccgtcatcat
    12601 cgacgaggcg cacgagcgca gtctcaacat cgatttcctg ctgggctatc tcaagcagct
    12661 gctgccgcgc cggccggacc tcaagctcat catcacctcg gccaccatcg acgccgagcg
    12721 cttcgcgacc catttcgcat cggaatccgg caagccggcg ccggtgatcg aggtgtcggg
    12781 gcgcctgtat ccggtggagg tgcgctaccg gcccgtgctg caggagcagc ccgatgacgc
    12841 ggcgccctcc ggcaagggca accgcgatcg cctggccgga gacgaagagc gcgacctgat
    12901 cgacgccatc gtcgacgccg tggacgaatg cgcgcgccac ggcccgggcg acgtgctggt
    12961 gttcctgccc ggtgagcgcg agatccgcga gtcggccgag gcgctgcgca agcatcatcc
    13021 ggcgggcacc gagatcctgc cgctctatgc ccgcctgtcg caggccgagc aggagcagat
    13081 cttccggccg cgcggcaatg cgcgccgcgt ggtgctgtcg accaacgtgg ccgaaacctc
    13141 gctcaccgtg ccgggcatcc gcttcgtggt cgacagcggc ctggcgcgca tcaagcgcta
    13201 ttcctggcgc aacaaggtcg agcagctgcg catcgagtcc atcagccgcg cctcggccaa
    13261 ccagcgcgcc gggcgctgcg gccggatagg gccgggccta tgcatacgcc tgtacgacga
    13321 ggccgacttc aacgcccgcg cgcctttcac cgaccccgaa gtgctgcgtt cctcgctggc
    13381 ctcggtgatc ctgcgcatga agtcgctggg attggacgac atcgagcgct ttcctttcgt
    13441 ggaggcgccg cccggccgtg ccgtggccga cggctaccat cttctgttgg agctgggtgc
    13501 gatcgctctg gcgcaggccg aggataaggg cgacgaggcc acgcgcacgg gggcggccta
    13561 cgagctgaca aacaccggcc gcgagctggc caagctgccg gtggacccgc gcatcggccg
    13621 catggtgctg gccgcgcgcg agcagcaatg cctggccgaa atgctcatca tcgcttcggc
    13681 cctgtcggtg caggacccgc gcgaccgccc catggccgag cgcgaagcgg ccgaggccgc
    13741 gcacgccaag ttcaatgacg acaagtccga attcgtcagc tttctcaagc tgtggcgctg
    13801 gtacggcgaa caggtgcagc acaagccgtc gcagcgccgg ctcgtgggcc ttttgcggca
    13861 gaacttcctg tcgccggtgc gcctgcgcga atggcacgac gtgcacacgc agctggccgc
    13921 cctggtgggc gagctgggct ggcgcctgaa ccagacggac gccacctacg agcaactgca
    13981 tctggccctg ctctcgggcc tgctgggcaa tatcggtttc aagagcgagg aaggcggcaa
    14041 ccccggcggc aattaccagg gcgcgcgcga gatccgtttc catatccatc cgagctccac
    14101 cctgcagaaa aaggccggcc gctggatcat ggccgccgag ttgatcgaga ccacccgcct
    14161 gtatgcgcgc tgcgtggccc gcatcgagcc cacctggctg gagcgcgtgg ccggccacct
    14221 gctgcgccat aactggtccg atccgcgctg ggaaaagaaa gccggccagg tcgtggccaa
    14281 cgagcgcgcc accttgtacg gcctgcagat ctaccatggc cggcgcgtgc actacggccg
    14341 catcaacccg cagcatgcgc gcgagctttt catccgcgag gccctggtgc cgggcgagat
    14401 cgacaccaag ctgcccttcg tcgcgcataa ccgcagactg atcgccagca tcgaaaagct
    14461 cgaacaccag acccgccgtc ccgacatcct ggtcgacgac gagctcatcc atgccttcta
    14521 cgaccagcaa ctgcccgcag gcatctcgca gacggccacg ctggaaaaat gggtccatgg
    14581 cctggacaag gctgccgccg acaagctgct gctcacgcgc gaagcgctca tgcgccatga
    14641 ggccgcgggc gtcaccaccg aggtctttcc caagaaagtg gaatggcagg gcgtgtccat
    14701 ggcgctcgac taccacttcg agcccggctc gtccaaggac ggcgtgaccc tgtcggtgcc
    14761 gctcttcgcg ctcaaccaga tcgacgcgca gcgctgcgag tggctggtgc ccggcatgct
    14821 caaggaaaag gtgcacctgc tgctcaagtc gctgccgcag aagctgcgcc gccactgcgt
    14881 gccgctgccc gattattcgg cgggtttcta tgaccgctgg ttcgataagc tgggcgatcc
    14941 gcagcagggc ctggtcgaag ccatcattgc cgacatgtgg gaccaggtgc aggtgcgccc
    15001 ggtgctgggc gacttcaagc tggagacctt gccggcgcat ctgttcatga acttccgcgt
    15061 ggtcgacgaa catggccgca tgctggccgc cgggcgcaac ctggcccagc tcaaggccga
    15121 gctgggccgc caggcgcagg ccaacttcca gcagctggcc tcgcgcgata ccgaggtggc
    15181 ccaggcgctg gcgcacgaaa agctcacgtc gtggtcgttc ggcccgctgc ccgaggtcat
    15241 ggagatcaag cgtggcggcc agtcggtcat cggctatccg gccctggtgg acaagggcgc
    15301 gcatgacgac ctcgatgtgt tcgacgaccc cgacgaggcg cgcaggcatc accgcgcagg
    15361 cctgttgaaa ttgttccgcc tgggtttgcg cgaacaggtg aagttcctgg aaaagaacct
    15421 cgccgacctc accaagatga gcatgttcta cctgaccctg ggcacgcagg aagaactgcg
    15481 cgaccagatc atcgattgcg cgctggcgca ggcctgcctg gccgagccct ggcccgccaa
    15541 cgaacagcaa ttcgaggcgc gccgcaacga aggcaagggc aggctgggcc tgctggcgca
    15601 agaggtggcg cgcctggccg gcactatcct ggccgaatac gccgccctgc aacgcaagct
    15661 gcagcaagcc aagccgcacg cggccgccca tgccgacctg cagcagcagc tgggcgcgct
    15721 catgcccaag tggttcatcc gcgagatccc ctacgcccag ctggcgcatt acccgcgcta
    15781 tctgaaggcg gcggtcgggc gcatcgacaa gctgcgcgcc gatccgggca gggatgccaa
    15841 gctcatgggc gagatggcgc cgctgctgac gaactaccag cgcgcccgtt cagccctgaa
    15901 gggcgcgccc gatccggcct tggacgagtt ccgctggctg ttggaagagc tgcgcgtggc
    15961 cttgttcgcc caggaactgc gcacgcccat gccggtgtcg gtgaagcggt tgatgaaggc
    16021 gtgggaagcg ctgcggcgct aggcgcctgc cgggccgggc gcatgggctg gaaatgacgg
    16081 ttgccggggc gcgctaatgt gcgctgtggt ttttcagcag gcgcactaac tcctcttcgg
    16141 ccatgcccag ggccagggtg gccgatcgcg ccagaatctg gctcaggccc tttatcgtcc
    16201 cggtttcaga caggccggcc agagatattc cttgggcaag ctgccagaca atggcgcgtg
    16261 ctctcgccgc ttccgttgcc ggatcgaggt agccgtattc ggccaggaaa tcatcgaagc
    16321 tggccttgcc gcgcaggttt ttcatgctgg gtccgtactt ggcgctcctg gtgcgctttt
    16381 cccttggagg gggcggcggg ctatgtgctt ttgcgatagc gcagcaaatg ccggaaacgg
    16441 atggccggcg cttcgatggc gttgaaggtg atgctgctca gaaccacgga acacaggacc
    16501 aggacgacga ttcttttggg cgaggtggct ggaaacgaca gcgcttcgaa cagccacatc
    16561 aggaagaaat gattgagaaa gacgccgtag ctgatattgc ccagcaaggt gtccaggcga
    16621 ctgttgggca ggttcctgag caggcagacc gccgggatgc cgatgaccag gcccgccagg
    16681 acctcggcgt tgttgggcag ctgcagggag ggctgctgtt ttaccgcggc gagcccaatg
    16741 gctgcgcagg cccagatcac ggcgagcgcg agtcgtcgat gtttgatctt gcggtcatag
    16801 atgaaactac cggtcaagaa gatgaacagt gttccgggca ggagccggta gccccataca
    16861 tcggtattga tcaggcccat gtaggccatc atgaacacaa gcgccgagag ggcgaaggcg
    16921 gctattcgca ggcgccagac cagcaggagc ggaatcgcca ggtagaacat ggtttccagt
    16981 cccagcgacc aggcttgcgg gatcagcatg gtgttctcca tgccgtacat gtacaagccc
    17041 aggggcagta tcagtacatt gagcaggatc ttcgacagac tgaaatcgaa cccctgcacg
    17101 aaggacgaca ccggcctggc cgccaggatc agcacgagag tggcggccag gtaggcgagg
    17161 tactgaggca gcagccgcat tgcccggtcc aggtagaagg gaaggatccg gccagcggcg
    17221 aaatagttct tctggatcaa ggcggtcatc acgaatccgc tgagcaacag gaacgagatg
    17281 accgcggtga tgcccggatt gagtccgaaa atgctgaccc cctgatggga aagcaagacg
    17341 aatatggcca ggatcaggcg gtaagtgccc atgagggagg agatgggcat ggatggaaaa
    17401 ggccgatgcc cggcgggttt cgaggttggg caatataaac ctattggttg tttgaaacaa
    17461 ccaaatggtt gccaataaat agctcattcc cgagacactt cgtgcatgca agccatggac
    17521 gacaagcagg cattcgccga gcggctgaaa aaggcgctca agcgcagcca caagaaaatc
    17581 gaaacgccga ctgacttggc tacccagttc agcctgcggt ccaatgagcc cgtcacgccc
    17641 caggcggcgc agaagtggct gtccgggcgc gccattccca cgcccgacaa gatctcgatc
    17701 ctggccgaat ggctcgatgt ctccgaaaaa tggctgcggc atggtgtggc agatgagccg
    17761 cggcattcgc atcgtggccg gcctgccgcg atgcgcgaac cccaagcgcc ttccgcggcg
    17821 gaatggacgc tgctggagcg cttgcgtcaa ttgcccgagg cgcgccgcaa attggtggaa
    17881 gacctggtcg agcagctttc cctggatgcg gaagtctgga agcgttcctg atcttccgga
    17941 cgcgggcgca gaccgggcaa gaggccggca tcagatcgga cctcccggga gactcctgag
    18001 agactgccgg ccctcgcggc cgttatagtg ctgcccactg cgcccgccat cgggtgcggg
    18061 caagatcgca gaactcgcag gagacagggc tcatgggcac gcagccacgc cagatccacc
    18121 tcaacgcctt cgacatgaat tgcgtcggcc acatccagca aggcctgtgg acgcacccgc
    18181 gcgaccagtc cacccgctac ctggacatcg actactggac atccctggcc cgcaagctcg
    18241 aagccggctt gttcgacggc ctcttcttcg ccgacgtgct gggcgtctac gacgtctacg
    18301 gcgcaagccc cgacgcggcc atccgcggcg cggtgcaggt gccggtcaac gatccggcgc
    18361 tgctcattcc cgccatggcc gcggccacga agcatctggg cttcggcatc acctgcaacc
    18421 tcacttacga gccgccctat gccttcgcgc ggcgcatgtc cacgctcgac cacctcacgc
    18481 gcgggcgcat aggctggaat atcgtcaccg gctatctgga tagcgcggcc cgcgccgccg
    18541 gcctgtcggg ccagatggcg cacgacgacc gctacgacct ggccgatgaa tatatgtcgc
    18601 tggtctacaa gttgtgggaa ggcagttggg aagacggcgc cgtgcaggcc gaccgggcaa
    18661 gcggacagta tgccgatccg gccaaggtgc atgtggtccg gcaccaaggc ccgcaataca
    18721 aggtcaatgc cctgcatctg gccgagccct cgccgcagcg cacgcccgtg ctctaccagg
    18781 ccggcgcctc cacccgtggc cgcttgttcg ccgccacgca tgccgagtgc gtcttcgtca
    18841 acggacagag caaggatggc gtcaagcgcc tggccgacga tatccgcgcc caggcgatcg
    18901 ccctgggccg cgcggccggc gacatcaaga tcttcatggg cgccacggtc atcaccggcc
    18961 gcaccgatgc cgaagcccgg gaaaagttcg aggaataccg ccgctacgcc agcagcgagg
    19021 cggcgctggc gcacgcggcg gcatcgatgg gcatcgattt ttccaagtac gacctggacg
    19081 aacccatccg caccgagtcc gatggcaagc aggccatcgt gtccaatgtc gaagccatgt
    19141 cgcgcagcgc cggcccggcc tggaccaagc gcaagctgct cgaacagatg gtgctgggca
    19201 gccggcaggc gccgtgggtg ggctcggccg gaaaaatcgc cgatgcgctg atcgcctgga
    19261 atgccgaagc cggcgtcgat ggcttcaacc tgtcgcgcac cgtggtgccg gaatgcttcg
    19321 atgacttcat cgagctggtg gtgcccgtgc tgcaggagcg cggcgcgtac aagacgtctt
    19381 atcgagaagg cccctaccgc cagaagctgt tcggccaggc tcgcctgccg gccacgcacg
    19441 cggcggcggc ccatcggcgc gggacgcagg cgtaacccat gccacagcag atgcgggtcc
    19501 ggcccggcgc ggggctgggc aagacgggtt cgctcatcgt gttgctgttg tgcgagatcg
    19561 gcgccatgtc ggtgtggttc tcttcggccg ccgcggtgct ggccttgcgg caggcgggcg
    19621 gcgtgagccc catggccgag gccttgctca ccagtgcggt gcaggcgggc ttcgtggcgg
    19681 gcacgctggc cagcgccttg ctggggctgg ccgatcgcta cgatccgcgc cggctgtttg
    19741 ccgtttgttc gctggcggcg ggcgcggcca cgttcgtgct tggcgccatc gcgcccgccg
    19801 gcatcgaggt gtatgcgctg cgttttctga ccggggcctg catggcgggc atttacccgg
    19861 tgggcatgcg cctggctgcc acctgggcca accgggacct tggcctgctc atgggactgc
    19921 tggtgggggc gctcacgctg ggcacggcca gcccgcacct ggtgtcgctg gcgggcgggg
    19981 ccgagtggca tgtggtgtat cgcgtggcgg gctgctgcgc cctgctggcc ggggcggcca
    20041 tcaatgtgtg cggcatcggg ccatccatga ggcgcggggc gagcctgcat tgggccaagg
    20101 ccgtgctggc ctggcgcgac cggccgctgc gcctggccaa cctgggctat ctcggccata
    20161 tgtgggagct ctatgccatg tgggcctggc tggccgtgtt catcaaggcc agcttcactg
    20221 agcgcggcct ggccgatccg caggccagcg ccgagtggct gacgtccgcc gccatcggcc
    20281 tgggctttgt cggcgcctgg ctgggaggcc tgctggccga ccgccatggc cgcaccgcgg
    20341 tcaccatagt cgcgatgatc gtcagcggcc tgtgcgcggc cagcatgggc tgggcctatg
    20401 gcggccccgt gtggctggtc gccgcgctgg ccgtgttgtg gggggtgtcg gtgatcgccg
    20461 actcagcgca attctcggcc agcgtcgcgg aactgagtcc gccggatgcc gtcggcacct
    20521 tgctgacggc gcagacttgc gcgggctttc tgctgacgct ggtcagcatc catgccgtgc
    20581 cctgggtggc ggcggcgctg ggctggcccc tggccttcgg catgctggcc gtcgggcctt
    20641 tcctgggctg cgtggcgatg gcgcggctgc gcgccgaccc ggccgccgtc cggctggcgg
    20701 gcggccgccg ctagcggccg atggcggcgc cgggctacag cgcaataaag cccgcgatca
    20761 ccaggacgat caggccgtat ttggtggcct tgtagaccgt gcggttccag gccttgaagg
    20821 ccttgcgctt ctggtcgatg agccagtgcg catgcgtcag cttgttgacc aggccggtct
    20881 ggtcgccgct gtcgttgggc gagctggcgg ccgacatgaa cacgcggctg aaccattggt
    20941 tgaaggcgtc ggcccagcgc cacttcagcg gacgcactac atcgcagaac aggatgatgc
    21001 ggttgatgtc cgaggtgttg tacgcctcgt ggatataggt ctcgtcgaag atcacgctct
    21061 ggccgtcgcg ccagctgtag cgttcgccgt cgacatcgat gtagcagcgg tcgtcgttgg
    21121 gcgtggtcag tcccaggtgg tagcgcagcg agcccgcgaa gggatcgcgg tgcgggttga
    21181 gtttgccgcc cggcggcagt tcggcgaaca tggcggcctt gacgctgggc agggtcttga
    21241 gcagggccac ggtcttgggg cagagctgct cggccgaggg gtggcgcgcg tcataccact
    21301 tcaggtagaa gcgcttccag ccgtacttga agaacgagtt gaagccgatg tcgtcgtgct
    21361 tctcggcggc cttgatgtgg cgcagctcgg ccatgtgcag ggcttcgtcg cggatggtct
    21421 gccagttgtc gtccagcgcc ttcagctcgg gaaactcgcg gctggtgagg tagggcgtgg
    21481 tgggcacgcg cgaggtcagc accatgaagg cgttgaccgg ggccaagatc accgagtggt
    21541 cgagcagttg gcgcgacagc ggcaggcgca ccctgccgcg gaaatgcgca aacaggatgg
    21601 aggctatcca cagtgcgggg atgaaccatt tcatggttgt ctcgtcaggt tctgaaaagg
    21661 atcagagaag ctcccattgt tacatatatg gggtgccctt tgcgctgctt gcaacagtat
    21721 gaaggaaggg ggctgggtac aatcctggca tgtccgaagc tgccccgatt tccgcccctc
    21781 tcgctgtcca gtccacgtcc gaatccgttg cggcgcccgc cttcgtgcac ctgcgcgtgc
    21841 actccgagtt ttcggtcgtg gacggcctgg tccgcattcc cgacctggtc aagcgcgtgg
    21901 ccagtctggg ccagccggcg gtggcgctga ccgacctctc caacctgttc ggcctgatca
    21961 agttctacaa gtcggcgcgc ggcgcggggg tcaagccggt ggccggctgc gatgtctgg
//
LOCUS       DASWPO010000324        33582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1237432, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000324 DASWPO010000000
VERSION     DASWPO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 33582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33582
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237432"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VGC69_17130"
     CDS             <1..1078
                     /locus_tag="VGC69_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0297057.1"
                     /translation="RVEILKVLMAGARILILVEPTAVLTDQEAARLLDMVRGLARGGA
                     AVALVTHRMSDVKAYADRVTVMRGGRSMRTVATDQASVAELVQLTVGESLAVPADPPR
                     TRRPEGPPRLMVRGLSSRAPHGARAALGGVDLALHGGEIYGLAGVGGNGQSELAAALM
                     GLDRDVSGEIVLQGHGDLKHMPNWRRRALRMAAIPADRYGLALAGPLSVAENFGIGRV
                     HEGRYGPAWRLDARRLAGDAQQAVEQFDVQGVRGVGQKAALLSGGNAQKLVLAREFGR
                     QPSVVLAHSPSRGLDVRAGAEVHARLRAARDAGAAVLLISEDLDEVLGLADRVGVMVR
                     GRIVAEFEQPVDRQAIGQAMVDHG"
     gene            1071..2174
                     /locus_tag="VGC69_17135"
     CDS             1071..2174
                     /locus_tag="VGC69_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297058.1"
                     /translation="MVEATSRARRVPLADRRFSVEVRQHMAWHRQALILAVALAAGLG
                     VSAAILVAAGVPADELLDEFVMQTLFDGQSLRAVLFQASPMILVGIAACLAFRARFWN
                     LGIEGQMIWGAIGATAVSIYELGPPGARLPLMLAASLALGALWAIVPAALKLRLGVNE
                     MISSLMLNYVAGNFLLHLVYGAWQDPKDAFPHSPQFRGFERLPEWFGGAGSSAIALAL
                     AVMLLAWWLVGASRAGLYLRFVHASPRVAEAVGVPVRRMTLAAVLLSGGLAGVAGFVV
                     AAGQEGRLTQSFYSGYGFSGILIAFLARNNPLAAMIVSLLVAMLFVAGRNLQVFYQIP
                     FSMVQLIEAIIVIGMASSDFFIRHRLRAVVSKV"
     gene            2177..3112
                     /locus_tag="VGC69_17140"
     CDS             2177..3112
                     /locus_tag="VGC69_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297059.1"
                     /translation="MGAYGIAANWLGNVPDFTVPYALAALGLIVCERAGTMALGAEGL
                     MLCGALAGIGAQLLFGQPGIALLLAMLAAGVVSLLFAAMVIFLRVNQVIAGLVLVFFC
                     QGLTSLLGSLLGWANRPVAGLGTLTLWPLSELPLVGRLFAQNIVVYLTPVIFLAVWWF
                     LSRSTAGLRLRAVGENPQAADAAGINVAAWRIGAIVAGSSLAGLAGAYLSLVSTKLWI
                     AGMVGGRGWIAVGLVIFARWSPWRALAGAVLFGCIEALIPQLAAAGVRLPQYFLLMTP
                     YVVTLAVMVWVSVARPGSDGEPGALGQPYLREERK"
     gene            complement(3182..4060)
                     /locus_tag="VGC69_17145"
     CDS             complement(3182..4060)
                     /locus_tag="VGC69_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0297060.1"
                     /translation="MKFATLQTPKGKFVSLVDAGSQRYWPVQDLIPGFTGDMVQLVQD
                     FDKIRAGLAPKGEGLPLSQATVLAPVDQPRRNIFCVGKNYYEHAKEFAQSGFDSSAKA
                     GEDIPEAPIVFTKPFSTVIGPGVKVPSHPTVAKQLDYEAELAVIIGKPGRGISKADAY
                     SHVFGYTIVNDFTARDLQKTHRQWFLGKSLDGFCPMGPYLVTADELDVENLDVKCWIN
                     GELRQDSNTSRLIFDIPTLIETISAGITLQPGDVIATGTPAGVGIGFTPPKFVKTGDV
                     MRIEITGLGSLTNEVD"
     gene            4229..4939
                     /locus_tag="VGC69_17150"
     CDS             4229..4939
                     /locus_tag="VGC69_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY0297061.1"
                     /translation="MHDIDVSPVVRTGGAKQLARYLAEGISNGTLAVGVKLPAERELS
                     ARFHASRGAVRRVLGELKDRGFIAQAVGSGTFVAAAPAAAAPAFDGTGTISPAELMQA
                     RLLIEPLMMPLIVQYGTAQDFARMGECIARSEAAGTVEEFEYWDGALHEALATATHNN
                     FFRQILALATQVREQGEWGRLKQKSLTAEHRAAYEAQHRSLVEALKNRDETAARQALG
                     QHLRQIQRNLFGGDPSEP"
     gene            complement(4953..5633)
                     /locus_tag="VGC69_17155"
     CDS             complement(4953..5633)
                     /locus_tag="VGC69_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY0297062.1"
                     /translation="MNASTLPDVEQARFNMVEQQIRPWNVLDLKVLDALFAVRREQYV
                     PSALRALAFSDLEIPLQINGVDTHETMLEPKFEARFAQDLQLQPTDCVLEIGTGSGYQ
                     AALLAHLARQVTTVEIDSRLSAFAQQNLQLNNVGNVKVETGDGSTGWGSTEYDAILVT
                     GSVPAVPDALKYQLRVGGRMIVVAGQAPVMTAWRITRTTAASFETEGLFETVIKPLRG
                     PSVSHFKF"
     gene            complement(5851..6678)
                     /gene="thiD"
                     /locus_tag="VGC69_17160"
     CDS             complement(5851..6678)
                     /gene="thiD"
                     /locus_tag="VGC69_17160"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY0297063.1"
                     /translation="MTTHRRIPNVLSIAGVDPSGGAGILADIKTMSALGAYGCAVAAA
                     LTAQNTQGVTGVWPVPPAFVAQQIDTLFADARIDAVKTGMLGQQPVIIAVADRLAHWQ
                     PAHFVLDPVMVASSGDLLLERNAVGVLGEALLPLATVLTPNLPEAGVLLDERPVESVK
                     EMRRVAERLRNQMAHSGQRWVLLKGGHLPGGEAAIDLLHDGDRMIELAAPRVDTRNTH
                     GTGCTLSAALAALLPQSGDVPEAAKRAKAYLTEALRHADRLDAGAGHGPVHHFHAWW"
     gene            complement(6730..7524)
                     /locus_tag="VGC69_17165"
     CDS             complement(6730..7524)
                     /locus_tag="VGC69_17165"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY0297064.1"
                     /translation="MNTDTLTIAGREYRSRLIVGTGKYKDFEQTRAALDASGAQIVTV
                     AIRRTNLGQTPGEPNLLDYVPMSTFTLLPNTAGCYTADDAVRTLRLARELLDGHDLVK
                     LEVLGDPQTLFPNMPETLKATKMLVDEGFKVMVYCADDPIQCRMLEDLGAATIMPLAS
                     LIGSGMGILNPWNLRLIIDQSRIPVLVDAGVGTASDVAIAMELGCDGVLTNSAIAHAQ
                     DPVRMGRAMKLAIEAGREAFLAGRMPRKLYSAIPSSPSEGLITAKR"
     gene            complement(7593..9119)
                     /locus_tag="VGC69_17170"
     CDS             complement(7593..9119)
                     /locus_tag="VGC69_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0297065.1"
                     /translation="MPAPSSHLLRPRLAGLCCALALALAGCAVGPDYRRPAVDMPAAF
                     KEAAGWKAAAPRDDLPRHAWWTVYSDPTLDGLMAQVEISNQNVAQYTAKYAQARALAD
                     QARASLFPSLTGSAAATRSADGSDGTSAGTSSARSASRVANSVSASLGLSWELDLWGK
                     LRRTREENQASAQASAADLANATLSAQSSLAQDYFSLRILDERINLYEQTIQAYEKYL
                     RIIENKYADGQVSSSDVAQARNQLESARASVQDLAWQRAQYEHAIAILAGRAPAAFGL
                     PRAIGAALRVPDIPVGLASALLERRPDIASSERAVAAANAAIGVATAAYFPDLTLSAS
                     GGFQGSSASKLFTVPSRFWSIGPSFGQTLFDFGATRAQVAQARAGYDAAVASYRQTVL
                     TALGEVEDYLVKLRTLDGELVSQQRATAAAIESARVTRDQYEEGMIDYLDVATTQATS
                     LNAQQSLWELQGAQLATSVQLIAALGGGWRQDGAQAGGDTDAGADGDPAGPKAAPRVL
                     "
     gene            complement(9129..12248)
                     /locus_tag="VGC69_17175"
     CDS             complement(9129..12248)
                     /locus_tag="VGC69_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0297066.1"
                     /translation="MNLSRPFILRPVATLLLSLALTLLGCLAYRLLPVASLPQVDYPT
                     IRVQASLAGASPETMAATVAAPLERAMGQIAGITEMTSSSSQGSTSVIVQFDLARDID
                     GAARDVQAAINAARSLLPSSLKTLPTYRKANPSDAPILVLALTSGTLGKGELYDLASS
                     KLQQRIAQVQGVGDVSLMGSALPAVRIELQPQQLTHYGISLDTVRAAVANATTNLPKG
                     ELSGDALHWWVDDNGQLTQAKDYRGLIVSYANGAAVRLSDVARVYDSVQDLYVAGYYN
                     DQPSVTLGVTRAAGANMLETIDAIKALTPVLNEMLPAGVTLTIANDRSLTIRGSLHET
                     ETTLLIAILLVIAVVFLFLRNSRALLIPAVALPISLVGTCAAMYLLGYSLDNLSLMAL
                     IVATGFVVDDAIVVLENISRYLEEGVPPLEAALRGSAEVGFTVLSMTISLIAVFIPIL
                     LMGSIVGRLFREFAVTLSVSLLISMVISLTLTPMLCARLLRPRAAGAPAANRPYRAIE
                     RALDGLARGYAASLAWVMRHKRLTLLTLLATVVLNLFLYTVVQKGFFPDQDTGLLIGR
                     LRADQNTSFQAMHPRLREFSRRIQQDPDVAAVMSSTGGGGFGSRNSGNFFVTLKDGSQ
                     RRDSAMQVANRLSRETAGVPGMSLFFMPAQDLRVGGRSANATYQYSLQSDDLDTLRAW
                     APKAYAVIRKLPQLTDVDTDAQNGGQEVRLVIDRQAASRYGLDVSDIDAFLNNAFSQR
                     QVATQYQMLNQYYAILGLAPAYTQDPAVLGQLFVLDDAGSAVPLSAFARMESANTALS
                     VAHQNQTATTTIAFNLADGVSLDQARAAIDQAVASIGLPASVHGSLQGTAKAFETLVA
                     AMPWLILAALLAVYIVLGMLYESWIHPLTILSTLPSAGVGALLLLIATGTQLTVIALI
                     GILLLIGIVKKNAILMIDFALHAERERGLDPEQAILEACRMRLRPILMTTLAAFCGAL
                     PMVLQSGGDAALRQPLGLAICGGLALSQLLTLYTTPVVYLYLDRLGSAGRRFWRARLR
                     QPAAPAPEN"
     gene            complement(12245..15451)
                     /locus_tag="VGC69_17180"
     CDS             complement(12245..15451)
                     /locus_tag="VGC69_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0297067.1"
                     /translation="MNPSRLFIRRPVATLLMMIAVLLTGLFAYRTLPISALPQVDYPT
                     IQVATLYPGAGPDVMASSVTAPLERQLGQMAGLSQMYSTSSGGASVITLKFNLDLSLD
                     VAEQEVQAAINAANSLLPAGLPNPPTYKKVNPAYTAVLTLAATSDSLPLTQVQDLVNT
                     RLALKLSQVSGVGLVSLAGGQQLAVRVRANPAALAAHGLTLQNLYNVINNGNANGAKG
                     GFDGPDHSVTIDANDQLRGADEYGGLVVTYEDGKALYLRDVATLEQAPENLYQAAWAG
                     DKPAIIVNIQRQPGANVVKVVDDIKALLPMLQQSLPGSVKLAVLSDRTQTIRASISDV
                     QSELLLSVALVVMVSFLFLRTLAATLIPSVAVPLSLVGTFGVMYLAGFSLNNLTLMAL
                     TIATGFVIDDAIVVVENIQRHLENGDPPMTAALKGSGEVSFTIISLTFSLIAVLIPLL
                     FMGDVVGRLFREFSITLAVSILVSMLVSLTLTPMLCAYLLRHIPPEQESRFSRLSGGG
                     FDRLLRAYGRGLAWVLDHQRLTLLAAVSTLALTGLLYLAVPKGFFPAQDTGQLLGITA
                     APQSVSFAEMSRRQQALTAVLLQDPDVQAISSVVGVDGANNTALNNGRLQIELKPFSE
                     RDDRAPAVIERLRATAAQVPGIQLYLTASQDLSVDDQVTPSQYQLTLDTPEQADLDAW
                     TPRLAAALSRKPALRDVASSLANRSPVAYVNIDRAAAARYGLTAADVDTALYNAYGQR
                     QVSTIFTQANQYRVVLEVDPRFRQDLSGFDQLYLAKSSGTSGSTTSTTSSTGSSTTSG
                     DSSSSSTSGAGAATAMVRLANIARIEERVGYQTRARLDQTPAATLSFNLAPGHSLEQA
                     RGAVAQAAREIGLPDTVMLRYQGAAQAFAASTGNTPWLILAAVATMYIVLGVLYESWI
                     HPVTVLSTLPSAAIGALLALLLSDSDFGLIALIGVILLIGIVKKNAIMMIDFALQAQR
                     EGKSAREAIHQACLLRLRPILMTTMAALLGAVPLMLAAGSGAELRRPLGLVIVGGLLL
                     SQLLTLFTTPVIYLFFDRLGTAVRRRWNARADTRSDAT"
     gene            complement(15486..16703)
                     /locus_tag="VGC69_17185"
     CDS             complement(15486..16703)
                     /locus_tag="VGC69_17185"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEY0297068.1"
                     /translation="MDPTSSTPTPRRRRWLAAASVLLVVLALAAYLLHARLGGVRSRP
                     ALDAAALVQAASATAADVPVALTALGTVSASASVTVTSRIDGHLQAVYFTEGQYVEKG
                     QLLAQIDTRPYQAELTQYQGQLAENQAQLANAQLTLARYQRLYAKDSLARQDLDTQLA
                     TARQYEGAVKASKGQAEAAQLNIDYGRITAPVDGYVGLRLVDPGNMVHASDTTGLVTI
                     TQTHPVTVTFSIPQANIQAVLPTLRQGRTLPVTALDQQGSQPIATGKVQFISNQIDAS
                     TGSVQLKAQFDNADDALYPNQFVNVRMQVDTLKNAVLVPAAAVQLSDSGKFVYLVGAD
                     DTVKRQSVATGPAAEDGRLVVLRGVQANDRVVTQGIDSLGNGSKVKVVEPRKVDASEL
                     DSAPQRRMGPPRR"
     gene            16851..18242
                     /locus_tag="VGC69_17190"
     CDS             16851..18242
                     /locus_tag="VGC69_17190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0297069.1"
                     /translation="MKSSLSTRLFLAMLALCALAILAMGFASYISFTQGFLGYLNEQA
                     EERIDVILPRFEKAYAKAGSWDFLRGRPNVWFFMTRPQEGPPGEGPPEPNISDLTGAF
                     LRVTLFDEMDNYVGGFRGYTKEQIMRPVMHDGHSVGWVGITPFESVSAAGGLRFQDSQ
                     LRSSVLIGIVCLLLAIVVAWRISLGVLKPVRSVAEATHRLAAGDYSIIAVPGQRNDEV
                     GRMAADFNRMATQLASNERKRREFMADISHELRTPLAVLRGELEAMEDGIRSVSSDTL
                     QSLQGEVAMMSKLVDDLFELSLAEIGGPTYRRDPVDLSDLLAQCMESFLPRLAARNIS
                     SLLEAPPGLQVRGDDRRLSQLFFNLLENSLRYTDPGGTVWWHATLEDQNVRIFCEDTA
                     PGVPESQQERLFERFYRVEASRNRASGGAGLGLAICRGIVEVHGGTIKAGDSSLGGLC
                     IEVRLPVLRTKAA"
     gene            18239..18916
                     /locus_tag="VGC69_17195"
     CDS             18239..18916
                     /locus_tag="VGC69_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0297070.1"
                     /translation="MNAPARILVVEDQAKLAALMVDYLRAGGYAVDHVADGNLAAQVL
                     REQVHDLLVLDLMLPGRDGLRICRELRDYSQMPVIMVTARVDEIDRLLGLELGADDYI
                     CKPFSPRELVARIKAVLRRYGPWSESRRQAMTIDPQAYQARYNGVLLDLTPVEFRLLA
                     ALHAGCGRVLSRDQLMTHLYEDHRVVTDRTVDSHVKNLRRKLEAAMPGQDPIHSVYGV
                     GYSLDLS"
     regulatory      18946..19165
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            19202..21052
                     /locus_tag="VGC69_17200"
     CDS             19202..21052
                     /locus_tag="VGC69_17200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0297071.1"
                     /translation="MSSVRLKQAAAFSPLLLSFVVPAHAQQTLDPIVVTATRQETRAS
                     ELLADVTVIDRAEIERHAGGTIADVLATQPGIQFARNGGPGTSTSFYVRGANSDQTKV
                     LVDGVPINSLDASGSSLPNISLADVDHIEIVRGPASTLYGADAVGGVIQIFTRKGEPG
                     VKFDSFIGYGTQNTLQANAGLSVGQEKWRFRFEGNRMSTSGISAQTGATNQDADKDGY
                     FNTGGAVSFALLPAEGHELGMSYRQNQGRAYYDSGNVPADGAFDDHVDFVTSQWQLYA
                     KDRLTSFWNSTLQFGQSLDWQKSYGEYAPEGTFLQTVSQQLSWQNDITLPLGKMLVAY
                     EHSDQQASADDSQPFTAGDYITNDSVLVGWTADWGKNRWQVNGRRDINSIYGSNNTYS
                     ASYGYQITPEWRAQASYGTAFKAPTLYQLYTPFYGNPLLKPESSRNREVSLVWERDEQ
                     RASLTYYRNSVTNLIDWVLIDPSSFAGAYENVEQATLQGVTLAYSGRFGDWRLHGSYD
                     WLDAVDDTTGMRLGRRARNKGVVGVSHIWGPVEAGAELVAAGSRFNTNDQTGTLGGYS
                     LVNLTASYAVSKSLSIQASVNNVFDKHYELAQGYGTLGISAFVGIRYTMQ"
     gene            21070..21663
                     /locus_tag="VGC69_17205"
     CDS             21070..21663
                     /locus_tag="VGC69_17205"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEY0297072.1"
                     /translation="MDLPSAFARTELVLGGQKSGKSRHAEERARAWLPACGGHRAVLV
                     ATGQARDAEMAARIARHRRDRALRVPGMATVEEPLLLAQKIAELSRPEVLVIVDCMTL
                     WLTNWLMPADGPGQDEPAPLPGFDAQAALLFGALAEARGPVILVSNEIGLGVIPMGRH
                     VRAFVDALGTLNQRLAQACERVTLVSAGLPLALKGRP"
     gene            21660..22517
                     /locus_tag="VGC69_17210"
     CDS             21660..22517
                     /locus_tag="VGC69_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEY0297073.1"
                     /translation="MRRLVLALLLLCGALPVHALEITDDRGVAVVLDGPPQRIVSLLP
                     SLSETVCGLGQCRRLVGVDRYSSYPDALKQLPQVGSGMEPNIEAIVALKPDVVLMAVA
                     SRVSARLESLGLKVVALDTETYADVQRVTQTVGALLGVDDAPRVWQAIELGVADAARS
                     LPPQVRGLRVYDEVGRLYAAGESSFIGETLKRLGVQNIVPASLGPFPKLNPEYVVKAD
                     PDLIIVSRNDAPDMMQRPGWSGIRAVREHRICALSPAQTDIVIRPGPRLPEGARILAD
                     CIAGKGLSQ"
     gene            22514..23533
                     /locus_tag="VGC69_17215"
     CDS             22514..23533
                     /locus_tag="VGC69_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY0297074.1"
                     /translation="MTRTLPAQHRRATLWGGGLAVLALCAGVLGMGIGSTGLENVWHL
                     RDDPSAWRIVWEIRLPRTLGAWLAGALLGLSGALAQGLFRNPLADPYLLGSASGASMG
                     VALVTAFGGISPLGAPWLARLGLTGAAFVGATCAVLLTLALARGAQQTLRLLLAGVIV
                     GVVLGACNSLMMLRYPSVMQIMQAFILGSTAFVDWGALGLMAVAWIVCALAAWSLSRV
                     LDGLTLGEETAASLGLPLAPLRAILIAVLALAIGTAVAQTGLIAFVGLASPHLVRSLV
                     KTTHNRLILLSSLMGGALLLAADLLARWVIAPQELPVGVLTALIGGVYLLWRMHAGNV
                     RAGGL"
     gene            23530..24297
                     /locus_tag="VGC69_17220"
     CDS             23530..24297
                     /locus_tag="VGC69_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297075.1"
                     /translation="MSPPAIAARALGVQLGGRPILHGIDLALGRGRWTSIIGPNGAGK
                     STLLKALAGLLPHQGDVLLPGLPPGEQGGRRKARHLAWLGQNEHGADDLTVYDLVMLG
                     RLPHQPWLSPPSDADREAVRAALDMMQAWTWRDRSLGQLSGGERQRVLLARALAVQAE
                     LLLMDEPLANLDPPHQVDWLLLVRGLVARGGTAVSVLHEIPLALQADDVVVMAQGRIR
                     YQGRCDARATHAAIEQVFDNRIQIRQVDGQWICLPRF"
     gene            24312..24881
                     /gene="cobO"
                     /locus_tag="VGC69_17225"
     CDS             24312..24881
                     /gene="cobO"
                     /locus_tag="VGC69_17225"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0297076.1"
                     /translation="MQIEQPPTEKPYEKSEGERRGLVIVNTGDGKGKSTAAFGLAMRA
                     HGRDKAVKIFQFMKVPTARFGEHRIFEKLGLPIEGLGDGFSWKSKDLDRSAQLARDGW
                     QRARAAILGGDYFMVVLDEVTYPLVYGWIPLADVLQTLRDRPAAVHVVLTGRRCPQEI
                     IDLADTVTEMAMVKHAFTQGIPAQRGIED"
     gene            24902..25873
                     /gene="cbiB"
                     /locus_tag="VGC69_17230"
     CDS             24902..25873
                     /gene="cbiB"
                     /locus_tag="VGC69_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEY0297077.1"
                     /translation="MPWRDGAHPWAFVLAVGLALLVDIALGEPPAAWHPVVWMGRYLE
                     AAGRRFAPARETPRGRPDVRAFARGTFYWCAGAALTLVLAAVLQAVLLAAPAWLAVAG
                     LALLLKPMLAWAMLRREVQAVELALADSLPAGRERLSRLCSRDVSGLSAEQVRETAIE
                     SLAENLNDSVVAPVFWFVLLGLPGAAVYRYANTADAMWGYLGMRGGRDWTWAGKWAAR
                     ADDALSWLPARITAALLALAAGGMPARRLRREAGRTPSPNSGWPMAAMALALGVHLRK
                     PGVYVLNQDGADPQHADTARAIRLAGRCVLALPVLAALAAWPGGMAA"
     gene            25870..26829
                     /locus_tag="VGC69_17235"
     CDS             25870..26829
                     /locus_tag="VGC69_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0297078.1"
                     /translation="MMRVHGGPDALGVPRWDFSTNGNACGPCPQVVAQLQAADIRSYP
                     DPAYQALRRALADFHGVEAWRIVPAASASEFIMRISACLARAGQGRVWLPALAYGEYA
                     HAARLWGLSRVDEPEQADLCWLCEPSSPLGGEEKQLGKVAGQDSVVVLDRAYEPLRLA
                     GNSPVRAQEAGRVWQLWSPNKALGMTGIRGAYAIAPLSGAALADRLAQLAPSWSLGAH
                     AVAMLAAWTAPAVQAWLARSRETLAGWKAAQLDLLKAWPCLPSETNFFCMRAALDAAF
                     LRARGIKLRDAASFGLPGHWRLSVQPPEAQAALFAALEAGGAL"
     gene            26826..28286
                     /locus_tag="VGC69_17240"
     CDS             26826..28286
                     /locus_tag="VGC69_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEY0297079.1"
                     /translation="MTARCVMVLGTTSGAGKSWLATALCRWYARQGLKVAPFKAQNMS
                     NNARVVAMGEIGSAQYFQALAARAVPDVRMNPLLLKPEADTRSQVVLLGKVDEALTRS
                     PWRQRSEQVWPHIAASLDALRAENDVVVIEGAGSPAEINLMDSDIVNMRVARHAQAQC
                     LLVTDIDRGGAFAHLYGTWALLPPQDRALVRGFVLNKFRGDPGLLAPAPAQIQALTGI
                     PVVAVLPMWRGHGLPEEDGVFDDRAVLAGQGQAGRTVAVVAYPHLSNLDEFEALKRVP
                     GLRLRWVRSLADLAGMGVEDWIVLPGSKHTSSDLAWLREQDLDQFVLQHAYCGGAVLG
                     ICGGLQMLGVGMADPHGVDGDARGLGLLPLSTTFALDKTVRRCCARFGAVAGAWGALS
                     GLPLRGYEIHHGQTRMVRDGARAEADGREVMPGLAWQNAAGNVLGLYVHGLFEDAAVL
                     RALLGGQGPDLDAVFDGLADFLERHADPGLLYALIP"
     gene            28304..29350
                     /gene="cobT"
                     /locus_tag="VGC69_17245"
     CDS             28304..29350
                     /gene="cobT"
                     /locus_tag="VGC69_17245"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY0297080.1"
                     /translation="MTFLVPAINNLDNPERAGRLRHALDNKTKPVGSLGRLEALAVRL
                     GTILQSESPELRAPQLVVFAADHGLAAQGVSAYPSDVTWQMVENFLAGGAAVSVLARQ
                     HGIALTVVDCGVRHDFAPRAGLLVRKVAAGTADALHAPAMTAAQCEQALENGKALMAD
                     LPGNALLLGEMGIGNTSCASLLLSRLAQLDLADCTGAGTGLDDAGVARKTDILRRVLA
                     RHDNARQPLDALAAFGGFEIATMVGAILQAAAENRVILVDGFIVSAAVLVASRLDPLV
                     LQRCVFAHRSGERGHALMLAALQGDPLLDLHLRLGEGSGAALAWPLLNSACVILRDMA
                     SFESAGVSREESAP"
     gene            29364..30176
                     /locus_tag="VGC69_17250"
     CDS             29364..30176
                     /locus_tag="VGC69_17250"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEY0297081.1"
                     /translation="MNALRHLLLALQFFTCIPLPGGVARWVGFSPAMQRASFAYFPLV
                     GALVGVVGAGCFLGVLRCLPAVGASAWIAAILGSAATAMLTGAFHEDGLADLADGLGG
                     QVSRERALEIMKDSRIGSYGSVALVLVLSLKVSLVAALAQVDAPLAAWALFAGHAFSR
                     LMPVGVAAILPNAREPAASKSTAVTARVGWLAVSGAVFWALLALAVAAWRQPGLFWMG
                     GMAACGVAWLAMCRLLRRRLQGFTGDALGATQQVCELAFYLGLLFGLSAARG"
     gene            30178..30735
                     /locus_tag="VGC69_17255"
     CDS             30178..30735
                     /locus_tag="VGC69_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0297082.1"
                     /translation="MKLWLVRHARPLIDPGVCYGRLDVAADPAATQACALSLAAALPP
                     DLRVLASGLRRARQLAQALLALRADLRLAVDERLGEMDFGQWEGVPWHDIPEDAVDRW
                     ARDFARHRFGGVESAADVLGRVAQALAAMSALDEAVWITHAGVIRAVHHLVRHGDGAA
                     PTAATWPSQAPAFGEWTVLTIGEQA"
     gene            30732..31265
                     /locus_tag="VGC69_17260"
     CDS             30732..31265
                     /locus_tag="VGC69_17260"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297083.1"
                     /translation="MKLGKRSDEICYPFIYETSHLCDYEIVTDELCGHIGAALSCLDA
                     SEPAMRDIAQDLGRLQPLAFHANGSIRGRLAVDEADIAWLQGRLSHYRDELDGWVQGF
                     VLPRGLPPVPQLHLARSAAKKAIRCLVRVEQEGREVPMVLPRLCNLMCNVFFTLTLVV
                     NRRRGFAETPFTSKSYG"
     gene            31377..>33582
                     /gene="metH"
                     /locus_tag="VGC69_17265"
     CDS             31377..>33582
                     /gene="metH"
                     /locus_tag="VGC69_17265"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY0297084.1"
                     /translation="MTYPKLPYPLSAYTHGAEFARQLSRRILILDGAMGTMIQRYKLT
                     EADFRGERFKDHAKDLKGDNELLSLVRPDVIAEIHKQYLEAGADVIETNTFGATAVAQ
                     SDYDLPELAYELNKASAQLARAACDAFSTPEHPRFVAGALGPQPKTASISPDVNDPGA
                     RNVTFDELRVAYVEQMNGLLDGGIDIVLIETIFDTLNAKAAIFAVEEVFEARGVRLPV
                     MISGTVTDASGRILSGQTVEAFWNSVRHARPVTIGLNCALGAALMRPYVAELSKLCDT
                     YVCVYPNAGLPNPMAETGFDEGPSDTAGLLEEFAKAGLVNMAGGCCGTTPDHIRAIAQ
                     KVTVLTPRSVPEVPVKTRLSGLEPVNIDEASLFVNVGERTNVTGSKMFARLIREEKYD
                     EALAVARQQVENGAQVIDINMDEAMLDSAACMKRFLNLIASEPDIARVPVMIDSSKWD
                     VIETGLKCVQGKPVVNSISMKEGEDIFRHHARLCRRYGAAVVVMAFDQQGQADTLERR
                     KTICGRAYKILVEEEGFPPEDIIFDPNVFAVATGIDEHNHYAVDFIEGARWIRQNLPH
                     ARISGGISNVSFSFRGNEPMREAIHTVFLYYAIQAGLTMGIVNAGQLGVYADLTPQLR
                     DLVEDVILDRAEPVGKTEAGDERTPTERLVLFADTVKGDGKKREEDLAWRAAAVEERL
                     KHALVLGITNFIVEDTEEVRQKIEAQGGRPIEVIEGPLMDGMNVVGDLFGEGK"
BASE COUNT         4871 a        10904 c        12229 g         5578 t
ORIGIN      
        1 gcgcgtcgag atcctcaagg tgctgatggc cggcgcgcgc atcctgatcc tggtcgagcc
       61 gaccgccgtg ctgaccgacc aggaggccgc gcgcctgctg gacatggtgc gcggcttggc
      121 gcgcggcggc gcggcggtgg cgctggtcac gcatcgcatg tcggacgtga aggcctatgc
      181 cgatcgcgtg acggtgatgc gcggcgggcg cagcatgcga acggtggcga cggaccaggc
      241 ctcggtcgcc gaactggtgc agcttacggt cggcgagtcg ctcgccgtcc cggcggaccc
      301 gccccggacc cggcgtccgg aaggaccgcc gcggctgatg gtgcgaggcc tgagctcgcg
      361 ggcgccgcat ggcgcccgcg cggcgctcgg cggcgtcgac ctggcgctgc acgggggcga
      421 gatctatggc ctggcgggcg tgggcggcaa tggccagagc gagctggcgg cggccttgat
      481 gggactggac cgggatgtct cgggcgaaat cgtcctgcag gggcatggcg acctgaagca
      541 catgcccaac tggcgccggc gcgccctgcg catggcggcg attccggcgg accgctacgg
      601 cctcgcgctg gccgggccgc tgtcggtggc ggagaacttc ggcatcggca gggtccacga
      661 aggccgctac ggcccggcgt ggcggctgga tgcccggcgc ctggccggcg atgcgcaaca
      721 ggcggtcgag cagttcgacg tgcagggcgt gcgcggcgtg gggcagaaag ccgcgctgct
      781 gtccggcggc aatgcgcaga aactggtgct ggcgcgggaa ttcggccgcc agccgagcgt
      841 ggtgctggcg cacagtccca gccgcggcct ggacgtgcgc gccggcgccg aggtgcacgc
      901 ccgtctcagg gcagcccgcg acgcgggcgc ggccgtgctg ctcatcagcg aggacctcga
      961 tgaagtgctg ggcctggcgg accgcgtggg cgtcatggtc cgcgggcgca tcgtggcgga
     1021 attcgaacag ccggtcgacc gccaggcgat cggacaggcg atggtggacc atggttgagg
     1081 caacttcgcg cgcgcggcgc gtccccctgg cggatcgccg cttttcggtc gaggtccggc
     1141 agcatatggc gtggcaccgg caggccctga tcctggccgt ggcgctggcc gcgggcctgg
     1201 gcgtgtcggc cgcgatcctg gtggcggcgg gcgtgccggc cgacgagttg ctcgacgagt
     1261 tcgtgatgca gacgctgttc gacggccaga gcctgcgggc ggtgctgttc caggcatcgc
     1321 cgatgatcct ggtcggcatc gccgcctgcc tcgcattccg ggcgcgtttc tggaatctcg
     1381 gcatcgaagg gcagatgatc tggggcgcta tcggcgccac cgcggtgtcg atctacgagc
     1441 tcggtccgcc cggcgcccgg ctgccgctca tgctggcggc cagcctggcg ctgggcgcgc
     1501 tgtgggccat cgtgccggcc gcgctcaagc tgcggctggg cgtcaacgaa atgatctcct
     1561 cgctgatgct caactatgtg gcgggcaatt tcctgctgca cctggtctac ggcgcctggc
     1621 aggatccgaa ggacgcgttt ccccattcgc cgcaattccg cggcttcgag cgcctgcccg
     1681 aatggttcgg cggcgccggc tcgtccgcca ttgccctggc cctggccgtc atgctgctcg
     1741 cctggtggct ggtgggcgcc agccgggcgg gcctgtacct gcgcttcgtg catgccagcc
     1801 cgcgcgtggc cgaggcggtc ggcgtgcccg tgcgccgcat gacgctcgcg gcggtgctgc
     1861 tctcgggcgg cttggcgggg gtcgccggct tcgtcgtcgc ggcggggcag gaaggcaggc
     1921 tgacgcagtc gttctattcc ggctacggct tctcggggat cctcatcgcc ttcctggccc
     1981 gcaacaatcc gctggccgcg atgatcgtgt ccctgctggt ggcgatgctg ttcgtcgccg
     2041 ggcgcaacct gcaggtgttc taccagatcc cgttttcgat ggtgcagctc atcgaggcga
     2101 tcatcgtgat cggcatggcc tcttccgatt tcttcattcg ccatcgcctg cgcgccgtgg
     2161 tttccaaggt ctgaacatgg gtgcctacgg tatcgccgcc aactggctgg gcaatgtgcc
     2221 cgacttcacc gtgccctacg ccctggccgc gctgggcctg atcgtatgcg agcgggcggg
     2281 gaccatggcc ctgggcgccg agggcctgat gctgtgcggc gcgctggccg gcatcggcgc
     2341 gcaattgctg ttcggccagc cgggcatcgc cctgctgttg gccatgctgg ctgccggcgt
     2401 ggtgtcgctg ctgttcgcgg cgatggtgat cttcctgcgg gtcaaccagg tgatcgccgg
     2461 cctggtgctg gtgtttttct gccaggggct caccagcctg ctcggctcgc tgctgggctg
     2521 ggccaaccgg cccgtcgcgg gcctcggcac cttgacgtta tggcccctgt cggagttgcc
     2581 gctggtcggc cgcctgttcg cccagaacat cgtggtctac ctcacgcctg tgatcttcct
     2641 ggccgtgtgg tggtttctgt cgcggtccac ggccggcttg cggctgcgcg ccgtcggcga
     2701 gaacccgcag gcggcggacg cggccggcat caatgtggcg gcctggcgta tcggggcgat
     2761 cgtggccggt tccagcctgg cggggctggc gggcgcctat ctttcgctgg tgagcaccaa
     2821 gctgtggatc gccggcatgg tgggcggccg cggctggatt gccgtcggcc tggtgatctt
     2881 cgcgcgctgg tcgccctggc gggcgctggc gggcgccgtg ctgttcggct gcatcgaggc
     2941 cctgattccc cagctggctg ccgccggcgt gcggctgccg cagtatttcc tgctgatgac
     3001 gccttacgtc gtgaccctgg ccgtgatggt ctgggtgtcg gtcgcccggc cgggttcgga
     3061 cggcgaaccc ggggcgctgg gacagcccta cctgcgggaa gaaagaaaat agcgccttcg
     3121 aagacgcgct ggcatttgca tcatgcgggc cctgccgcag ccgggcccgc gggtcgatcg
     3181 gtcagtcgac ttcgttggtc agcgaaccca gtccggtgat ctcgatgcgc atcacgtcgc
     3241 cggtcttgac gaacttgggc ggcgtgaagc cgatgcccac gccggccggc gtgccggtgg
     3301 cgatcacgtc gccgggctgc agcgtgatgc cggcggaaat ggtttcgatc agcgtgggga
     3361 tgtcgaagat gagccggctg gtgttggagt cctggcgcag ttcgccgttg atccagcact
     3421 tcacgtccag gttctcgacg tcgagctcgt cggcggtgac caggtagggg cccatggggc
     3481 agaagccgtc cagcgacttg ccgaggaacc actggcggtg cgtcttttgc aggtcgcggg
     3541 cggtgaagtc gttgacgatg gtgtagccga acacgtggct gtaggcgtcc gccttgctga
     3601 tgccgcggcc gggcttgccg atgatcacgg cgagttcggc ttcgtagtcg agctgcttgg
     3661 ccacggtcgg gtggctgggc actttcacgc ccgggccgat caccgtggag aagggcttgg
     3721 tgaagacgat gggcgcttcg gggatgtctt cgccggcctt ggcgctgctg tcgaagccgg
     3781 actgggcgaa ttccttggcg tgttcgtagt agttcttgcc cacgcagaag atgttgcggc
     3841 ggggctggtc caccggggcc aggacggtgg cctgggaaag cggcaggcct tcgcccttgg
     3901 gcgcgagtcc ggcgcggatc ttgtcaaaat cttgtaccaa ttggaccatg tcgcccgtaa
     3961 acccggggat gaggtcctgc accggccagt atcgctggga tccagcatca accagggaga
     4021 cgaacttgcc ttttggcgtt tggagcgtgg cgaatttcat gcggtcttct gtcgtgtgta
     4081 gagggtggat cgaatggatt ccaaggtaga atgtagtacc aatttgtgga ttcggcaatc
     4141 tatatcttga ttttgtggat ccaattggtt ctggatggta tgcttccctg cacattggac
     4201 cggatagaac ccgaatcgag gattttccgt gcacgacatc gacgtgagtc ccgtggtccg
     4261 caccggcggc gcgaaacagc ttgcccgcta cctggccgaa gggatcagca acggcacgct
     4321 ggccgtgggc gtcaagctgc cggccgagcg ggaactgagc gcgcggttcc atgcgtcgcg
     4381 gggggccgtg cgcagggtcc tgggcgaatt gaaggaccgg ggcttcatcg cgcaagccgt
     4441 gggcagcggg actttcgtgg ccgccgcgcc agccgccgcc gcgccggcct tcgacgggac
     4501 cggcacgatc agcccggcgg agctgatgca ggcccggcta ttgatcgagc cgctgatgat
     4561 gccgctgatc gtgcaatacg gcacggcgca ggattttgcc cggatgggcg agtgcatcgc
     4621 ccggtcggag gccgccggga cggtggaaga gttcgaatac tgggacggcg ccctgcatga
     4681 ggcgctggcg acggccacgc acaacaattt cttccggcag atcctggcgc tggcgacgca
     4741 ggtccgcgag cagggggagt ggggacgcct gaagcagaag tccctcacgg cggaacaccg
     4801 cgcggcctat gaggcgcagc accgctcttt ggtggaggcc ctgaagaacc gggacgagac
     4861 cgccgcgcgc caggcgctgg gacagcatct gcgccagatt cagcgcaatc tgtttggcgg
     4921 cgatccgtcc gaaccgtaac tggcggggcc ggtcagaact tgaagtgcga caccgacggc
     4981 ccgcgcaggg gcttgatgac ggtttcgaac aggccttcgg tctcgaagct ggcggcggtg
     5041 gtgcgggtga tgcgccaggc cgtcatgacg ggcgcctggc cggcgaccac gatcatgcgg
     5101 ccgcccacgc gcagttggta cttgagcgcg tcgggcacgg cgggcaccga gccggtgacc
     5161 aggatggcgt cgtattcggt gctgccccag ccggtgctgc cgtcgccggt ttcgaccttg
     5221 acgttgccga cattgttgag ctgcaggttc tgctgggcaa aggcggacag gcggctgtcg
     5281 atctcgaccg tggtgacctg gcgggccagg tgcgccagca gcgcggcctg gtagcccgag
     5341 ccggtgccga tctcgagcac gcagtcggtg ggctgcagtt gcaggtcttg cgcgaagcgg
     5401 gcttcgaact tgggctccag catggtctcg tgggtgtcga ccccgttgat ctgcaggggg
     5461 atctccaggt cggagaaggc cagggcgcgc agggcggacg gcacgtattg ctcgcggcgc
     5521 accgcgaaca gggcatcgag gaccttgagg tcaagcacgt tccaggggcg gatctgctgt
     5581 tccaccatgt tgaagcgggc ttgttcgacg tcgggcaagg tagaggcgtt catgacggct
     5641 gtggctatct tgtagggggg ataacccttg aattttaacg ctttgtagct gtttgggttc
     5701 ttaaggcgtc cactggggaa aaaggcccat ggcggcttcg ctgagccagg ctcgggcgcg
     5761 ccatcaggcg ccctcggccc gctacgcggg cttccctgtc ccagaggcgc cgccatgggc
     5821 ctttttcccc agtggactat gacgtgccaa tcaccaccaa gcatggaaat ggtgcaccgg
     5881 gccatggccc gcgcccgcgt ccagccggtc ggcgtggcgc agggcttcgg tgaggtaggc
     5941 cttggcgcgt ttcgccgcct cgggcacgtc gccgctctgc ggcagcaggg cggccagggc
     6001 ggccgagagg gtgcagccgg tgccgtgggt gttgcgggtg tccacgcgcg gcgcggcgag
     6061 ctcgatcatg cggtcgccgt cgtgcagcag gtcgatggcg gcttcgccgc cgggcaggtg
     6121 gccgcccttg agcagcaccc agcgctggcc gctgtgggcc atctggttgc gcaggcgctc
     6181 ggccacgcgg cgcatttcct tgacgctttc gaccgggcgt tcgtccagca gcacgccggc
     6241 ttcgggcagg ttgggggtga ggacggtggc caagggcagc agggcttcgc ccaggacgcc
     6301 gacggcgttg cgttccagca gcaggtcgcc gctggaggcc accatgaccg ggtcgagcac
     6361 gaagtgggcg ggctgccagt gcgcgagccg gtcggccacg gcgatgatga ccggctgttg
     6421 cccgagcatg ccggtcttga cggcgtcgat gcgcgcgtcg gcaaagagcg tgtcgatctg
     6481 ctgggccacg aaggcgggcg gcacgggcca gacgccggtg acgccctggg tgttctgcgc
     6541 ggtgagcgcg gcggccacgg cgcagccgta ggcgcccagc gcgctcatgg tcttgatgtc
     6601 ggccaggatg ccggcgccgc cggacgggtc cacgccggcg atgctcagga cgttgggaat
     6661 acggcgatgg gtggtcatga gcgtggcgca gcaagccgaa ggcgcggcgt ggggggcatt
     6721 gcttactttt tatctttttg cggtgatcag cccttcggag ggagagctgg ggatggcgct
     6781 gtagagcttc ctgggcatgc ggccggccag gaaggcttcg cggccggcct cgatggccag
     6841 cttcatggcg cggcccatgc gcaccggatc ctgcgcgtgg gcgatggcgc tgttggtcag
     6901 cacgccgtcg cagcccagct ccatggcgat ggcgacgtcg gatgccgtgc ccacgccggc
     6961 gtccaccagc accgggatgc gtgactggtc gatgatcagg cgcaggttcc aggggttgag
     7021 gatgcccatg cccgagccga tcagcgaggc cagcggcatg atggtcgcgg cgccgagatc
     7081 ttcgagcatg cggcactgga tggggtcgtc ggcgcaatac accatgacct tgaagccctc
     7141 gtcgaccagc atcttggtgg ccttgagggt ctcgggcatg ttgggaaaga gcgtctgcgg
     7201 atcgcccagc acttcgagct tgaccaggtc gtggccgtcg agcagctcgc gcgccaggcg
     7261 cagggtgcgc acggcatcgt cggcggtgta gcagccggcg gtgttgggca gcagcgtgaa
     7321 cgtcgacatc ggcacgtagt cgagcaggtt gggctcgccg ggggtctggc cgaggttggt
     7381 gcggcggatg gccacggtga cgatctgcgc gccgctggcg tccagcgcgg cgcgggtctg
     7441 ttcgaagtct ttgtatttgc cggtgccgac gatcaggcgc gagcggtatt cgcggccggc
     7501 gatggtgagg gtgtctgtat tcatggggac cacctgggaa ctgccgaata gggccgcggg
     7561 gccggcggac ccggccccgc gcatcgcctc catcatagaa cacgcggcgc ggctttcggc
     7621 ccggccgggt cgccgtccgc gccggcgtcc gtatcgccgc cggcttgcgc gccgtcctgc
     7681 cgccagccgc cgcccagcgc cgcgatcagc tgcacgctgg tggcgagctg ggcgccctgc
     7741 agttcccaca ggctttgctg cgcgttcagg ctggtggcct gggtggtggc cacgtccagg
     7801 tagtcgatca tgccttcctc gtactggtcg cgagtgaccc gcgccgactc gatggcggcc
     7861 gccgtggcgc gctgctgcga gacgagttcg ccgtccaggg tgcgcagctt gaccaggtag
     7921 tcctccactt cgcccagcgc ggtcagcacg gtctggcggt agctcgccac ggcggcgtcg
     7981 tagccggcgc gcgcctgggc gacctgggcc cgggtggcgc cgaagtcgaa cagcgtctgg
     8041 ccgaaggacg ggccgatcga ccagaagcga gagggcacgg tgaacagctt gctggcgctg
     8101 ctgccctgga agccgccgct ggcgctcagc gtgaggtcgg ggaaataggc cgcggtggcc
     8161 acgccgatgg cggcgttggc ggccgccacg gcgcgttcgg acgaggcaat gtcgggacgc
     8221 cgttccagca gggcggaggc cagtcccacc gggatgtccg gcacacgcag cgccgcgccg
     8281 atggcgcgcg gcaggccgaa ggcggcgggc gcccggccgg ccaggatggc gatggcgtgc
     8341 tcgtattgcg cccgctgcca ggccaggtcc tgcacggagg cgcgggcgct ttccagctgg
     8401 ttgcgcgcct gggccacgtc ggagctcgag acctggccgt cggcatactt gttctcgatg
     8461 atccgcaagt acttctcata ggcctggatg gtctgctcgt acaggttgat gcgctcgtcg
     8521 aggatgcgca gcgagaagta atcctgcgcc agcgaggact gcgcgctgag cgtggcgttg
     8581 gccaggtccg ccgcgctggc ctgggcgctg gcctggtttt cttcccgggt gcggcgcagc
     8641 ttgccccaca agtccagttc ccagctcagg ccgagcgaag cggatacgct gttggcgacg
     8701 cgggaggccg agcgggcgct gctggtaccg gcactggtgc cgtccgagcc gtcggcgctg
     8761 cgggtggcgg ccgcgctgcc ggtgaggctg gggaacaggc tggcgcgcgc ctggtcggcc
     8821 agggcccgcg cctgggcgta tttcgccgtg tactgggcga cattctggtt ggagatctcg
     8881 acctgcgcca tcaggccgtc gagcgtcgga tcgctgtaga ccgtccacca ggcatggcgc
     8941 ggcaggtcat cccgcggcgc ggcggccttc cagccggcgg cttccttgaa ggcggcgggc
     9001 atatcgaccg cggggcggcg gtagtcgggg cccacggcgc agccggcgag ggccagcgcc
     9061 agcgcgcagc acaggccggc caggcgcgga cggaggaggt gggaggaagg cgcgggcatg
     9121 gcggtcggtc agttttccgg ggcgggggcg gcgggctggc gcagccgcgc gcgccagaag
     9181 cgccggccgg cgctgcccag gcggtccagg tagagataga cgacgggcgt ggtgtagagc
     9241 gtcagcagct ggctcagcgc caggccgccg cagatggcca ggcccagagg ctggcgcagc
     9301 gcggcgtcgc cgccgctttg cagcaccatg ggcagcgcgc cgcagaaggc cgccagcgtg
     9361 gtcatcagga tggggcgcag gcgcatgcgg caggcctcga ggatggcctg ttccggatcc
     9421 aggccgcgct cgcgctcggc gtgcagggcg aaatcgatca tcaggatggc gttcttcttg
     9481 acgatgccga tcaggagcag gatgccgatc agggcgatga ccgtcagctg cgtgccggtg
     9541 gcgatcaaga gcagcagcgc gcccacgccg gccgagggca gggtggagag gatggtcagc
     9601 gggtggatcc agctctcgta gagcatgccc agcacgatgt acaccgccag cagggccgcc
     9661 aggatgagcc agggcatggc ggcgaccagc gtctcgaagg ccttggccgt gccctgcagg
     9721 ctgccgtgca cgctggcagg caggccgatg ctggcgacgg cctggtcgat ggccgcgcgg
     9781 gcctggtcca gcgacacgcc gtcggccagg ttgaaggcga tggtggtggt ggcggtctgg
     9841 ttctggtgcg ccaccgacag cgcggtattg gcgctttcca tgcgcgcgaa ggccgacagc
     9901 ggcacggcgc tgccggcatc gtcgagcacg aagagctggc ccagcaccgc cgggtcctgg
     9961 gtgtaggccg gcgccaggcc caggatggcg tagtactggt tgagcatctg gtactgcgtg
    10021 gcgacctgcc gctggctgaa cgcgttattg aggaaggcgt cgatgtccga cacgtccagg
    10081 ccgtagcggc tggcggcctg gcggtcgatg accagcctca cttcctggcc gccgttctgg
    10141 gcgtcggtgt cgacgtcggt cagctgcggc agcttgcgga tcacggcgta ggccttgggc
    10201 gcccaggcgc gcagcgtgtc caggtcgtcc gattgcaggc tgtactggta ggtggcgttg
    10261 gcgctgcgcc cgcccacgcg caagtcctgg gcgggcatga agaagagcga catgcccggg
    10321 acgccggcgg tttcgcggct caagcggttg gcgacctgca tggcgctgtc gcggcgctgg
    10381 ctgccgtcct tgagcgtcac gaagaaattg ccgctgttgc gcgagccgaa gccgccgccg
    10441 ccggtcgagg acatcaccgc cgccacgtcg gggtcttgct ggatgcgccg gctgaattcg
    10501 cgcagcctgg ggtgcatcgc ctggaaggac gtgttctggt cggcgcgcag gcgtccgatc
    10561 agcaggccgg tgtcctggtc ggggaaaaag cccttttgca ccaccgtgta gaggaacagg
    10621 ttcaagacca cggtggcgag cagggtgagc agcgtcagcc gcttgtgccg catcacccag
    10681 gccaggctgg cggcgtagcc ccgcgccaac ccgtccagcg cccgctcgat ggcgcggtac
    10741 ggccggttcg cggctggcgc gccggccgct cgcggccgca gcaggcgcgc gcacagcatg
    10801 ggcgtgagcg tgagcgagat caccatggag atgagcagcg acaccgacag cgtcacggcg
    10861 aactcgcgga acagccggcc gacgatgctg cccatcagca ggatggggat gaacaccgcg
    10921 atcagcgata tcgtcatgga cagcacggtg aagcccactt ccgcgctgcc ccgcagggcg
    10981 gcttccaggg gtggaacgcc ttcttccaga tatcggctga tgttctccag cacgacgatg
    11041 gcgtcatcga ccacgaagcc ggtggcgacg atcagcgcca tcagcgacag gttgtccagg
    11101 ctgtagccca gcaggtacat ggccgcgcag gtgccgacca gcgagatcgg cagggccacg
    11161 gccgggatga gcagggcgcg gctgttgcgc aggaacagga acaccacggc gatcaccagc
    11221 aggatggcga tcagcagggt ggtctcggtc tcgtgcagcg agccgcgtat cgtcagcgag
    11281 cggtcgttgg cgatggtgag cgtcacgccg gcgggcagca tttcgttgag caccggcgtc
    11341 agcgccttga tggcgtcgat ggtctccagc atgttggcgc cggcggcgcg ggtcacgccg
    11401 agcgtgaccg agggctggtc gttgtagtag cccgcgacgt acaggtcctg caccgagtcg
    11461 tagacgcgcg ccacgtccga cagccggacc gccgcgccgt tggcgtagct gacgatcagg
    11521 ccgcgatagt ccttggcctg ggtgagctgg ccgttgtcgt ccacccacca gtgcagcgca
    11581 tcgcccgaca gttcgccctt gggcaggttg gtcgtggcgt tggccaccgc cgcgcgcacc
    11641 gtgtccaggg agatgccgta gtgggtcaac tgctgcggct gcagctcgat gcgcaccgcc
    11701 ggcagcgcgc tgcccatcag cgacacgtcg cctacgccct gcacctgggc aatgcgctgc
    11761 tgcagcttgc tggaggccag gtcgtagagc tcgcccttgc ccagcgtgcc ggaggtcagc
    11821 gccagcacca ggatgggcgc gtcggacggg ttggccttgc ggtaggtggg cagggtcttg
    11881 aggctgctgg gcagcaggct gcgcgcggcg ttgatggcgg cctgcacgtc gcgggcggcg
    11941 ccgtcgatgt cgcgggccag gtcgaactgg acgatgacgc tggtggagcc ctgcgagctg
    12001 ctcgaggtca tctcggtgat gccggcaatc tgtcccatgg cccgttccag cggcgcggcc
    12061 accgtcgccg ccatggtttc cgggctggcg cccgccaggc tggcctgcac cctgatggtg
    12121 ggatagtcga cctgcggcag cgacgccacc ggcagcagcc ggtaggccag gcagcccagc
    12181 agcgtcagcg ccaggctcag gagcagcgtg gcgaccgggc gcaggatgaa cgggcgcgac
    12241 aggttcatgt cgcgtcgctc cgtgtgtcgg ccctggcgtt ccagcgccgc ctgacagccg
    12301 tgccgaggcg gtcgaagaac aggtagatca ccggcgtggt gaagagcgtg agcaattggc
    12361 tcaacagcag gccgccgacg atcaccaggc ccagcggccg gcgcagttcg gcgcccgagc
    12421 ccgcggccag catcagcggc acggcgccca gcagcgccgc catggtggtc atcaggatgg
    12481 gccgcaggcg cagcaggcag gcctgatgga tggcctcgcg cgcggacttg ccctcgcgct
    12541 gcgcctgcag ggcgaagtca atcatcatga tggcgttctt cttgacgatg ccgatgagca
    12601 ggatcacccc gatcagcgcg atcaggccga agtcggaatc gctcaggagc agcgccagca
    12661 gcgcgccgat ggccgccgag ggcagggtgg agagcaccgt caccggatgg atccagctct
    12721 cgtagagcac gcccagcacg atgtacatgg tggccaccgc cgccaggatc agccacggcg
    12781 tgttgccggt ggaggcggcg aaggcctgcg cggcgccctg gtagcgcagc atgacggtgt
    12841 cgggcaggcc gatctcgcgg gcggcctggg ctaccgcgcc gcgcgcctgc tccagcgagt
    12901 ggccgggcgc caggttgaac gacaaggtgg ccgccggggt ctggtccagg cgtgcgcggg
    12961 tctggtagcc gacgcgctcc tcgatgcggg cgatattggc caggcgcacc atggcggtcg
    13021 cggcgccggc accggaggtc gagcttgagc tcgaatcgcc gctcgtcgtg ctgctgccgg
    13081 tactgctggt cgtgctcgtc gtgctgccgg aggttccgct gctcttggcc aggtagagct
    13141 ggtcgaagcc ggacaggtcc tggcgaaagc gcgggtccac ttccagcacc acccggtact
    13201 ggttggcctg ggtgaagatg gtggagacct ggcgctggcc ataggcgttg tacagggccg
    13261 tgtcgacgtc ggccgcggtc aggccgtagc gcgccgcggc ggcgcggtcg atgttgacat
    13321 aggccaccgg gctgcggttt gccaggctgc tggccacgtc gcgcagcgcc ggcttgcgcg
    13381 acagggccgc ggccaggcgc ggcgtccagg cgtccaggtc ggcctgttcg ggcgtgtcca
    13441 gcgtcagctg gtattggctg ggcgtgacct ggtcgtctac gctcaagtcc tggctggcgg
    13501 tcaggtacag ctggatgccg ggcacctggg cggccgtcgc gcgcagccgc tcgatcaccg
    13561 ccggcgcgcg gtcgtcgcgt tccgagaagg gcttgagttc gatctgcagc cggccgttgt
    13621 tcagcgcagt attgttggcg ccgtccaccc cgaccaccga cgaaatggcc tgcacgtcgg
    13681 ggtcttgcag cagcacggcc gtcaaggcct gctggcggcg cgacatctcg gcgaaggaca
    13741 cgctctgcgg cgcggcggtg atgcccagca gctggccggt gtcctgggcg ggaaagaacc
    13801 ccttgggcac ggccagatac aggaggccgg tgagcgccag cgtggacacc gccgccagca
    13861 gggtcagccg ctggtggtcc agcacccagg ccaggccgcg cccgtaggcg cgcagcaggc
    13921 ggtcgaagcc gccgccgctc aggcgcgaga agcggctttc ctgttcgggc ggtatgtggc
    13981 gcagcaggta ggcgcacagc atgggcgtga gcgtcagcga caccagcatg gacaccagga
    14041 tggacaccgc cagcgtgatg gagaactcgc ggaacagccg gcccaccacg tcgcccatga
    14101 agagcagggg aatgagcacc gcgatcagcg agaaggtcag cgagatgatg gtgaagctga
    14161 cttccccgga gcccttgagc gccgccgtca tgggcgggtc gccgttttcc aggtggcgct
    14221 ggatgttctc gaccacgacg atggcgtcgt cgatcacgaa gcccgtggcg atggtgagcg
    14281 ccatcagcgt gaggttgttc agcgagaagc cggccaggta catcacgccg aaggtgccga
    14341 ccagcgacag cggcacggcc acgctgggga tcagcgtggc cgccagcgtg cgcaggaaga
    14401 ggaacgacac catcaccacc agcgccaccg acagcagcag ctcggactgc acgtccgaga
    14461 tggaggcgcg gatggtctgg gtgcggtcgg acagcaccgc cagcttgacc gagccgggca
    14521 ggctttgctg cagcatgggc agcagggcct tgatgtcgtc gaccaccttc accacgttgg
    14581 cgccgggctg gcgctggatg ttgacgatga tggccggctt gtcgcccgcc caggccgcct
    14641 ggtagaggtt ctccggcgct tgttccagcg tggcgacgtc gcgcaggtag agcgccttgc
    14701 cgtcttcgta ggtcaccacc aggccgccgt attcgtcggc gccgcgcaac tggtcgttgg
    14761 cgtcgatggt caccgagtgg tcggggccgt cgaagccgcc cttggcgccg ttggcattgc
    14821 cgttgttgat gacgttgtac aggttttgca gcgtcaatcc gtgcgctgcc agggcggcgg
    14881 ggttggcgcg cacgcgcacg gccagctgct gcccgccggc caggctgacc agccccacgc
    14941 ccgacacttg cgacagcttg agcgccaggc gggtgttgac caggtcctgg acctgggtga
    15001 gcggcagcga gtcggaggtg gccgccaggg tcagcacggc cgtgtaggcc gggttcacct
    15061 tcttgtaggt gggcgggttg ggcaggccgg cgggcagcag ggaattggcg gcgttgatgg
    15121 ccgcctgcac ttcctgctcg gccacgtcca gcgagaggtc gaggttgaac ttcagcgtga
    15181 tgacggatgc gccgccggag ctggtggagt acatctgcga caggccggcc atttgcccca
    15241 gctggcgctc gagcggcgcc gtgaccgaac tggccatgac gtcgggtccg gcgccggggt
    15301 acagcgtggc gacctgaatg gtggggtagt ccacttgcgg cagggccgag atcggcaagg
    15361 tgcgataggc gaacaggccg gtcagcagca ccgcgatcat catcagcaag gtcgccacgg
    15421 gccgccggat gaaaagacgg gaggggttca tggcctggac gggctctctt cggcgcggcc
    15481 gggcgtcagc gccgcggcgg tcccatgcgc cgctggggcg cgctgtccag ttcgctggcg
    15541 tccactttcc gcggctccac caccttgacc ttgctgccgt tgcccagcga gtcgatgccc
    15601 tgggtgacca cgcggtcgtt ggcctgcacg ccccgcagga cgaccaggcg gccgtcctcg
    15661 gcggccggcc ccgtggcgac ggactgccgc ttgaccgtat cgtcggcgcc cacgaggtag
    15721 acgaacttgc ccgagtcgct cagctgcacc gccgcggccg gcaccagcac ggcgttcttc
    15781 aaggtgtcca cctgcatgcg cacattgacg aactggttgg gatagagcgc gtcgtccgcg
    15841 ttgtcgaatt gcgctttcaa ctgcacgctg ccggtgctgg cgtcgatctg gttgctgatg
    15901 aattgcacct tgcccgtggc gatgggctgg ctgccctgct ggtccagcgc cgtcaccggc
    15961 agggtcctgc cctggcgcag cgtcgggagc accgcttgga tattggcctg cggtatgctg
    16021 aacgtgacgg tgacggggtg cgtctgggtg atggtgacca ggccggtggt gtcgctggcg
    16081 tgcaccatat tgcccgggtc gaccaggcgc aagcccacat agccgtcgac gggcgcggtg
    16141 atgcggccgt agtcgatgtt gagctgggcc gcctcggcct gtcccttgga ggccttgacc
    16201 gcgccctcgt actggcgggc ggtggccagc tgggtgtcca ggtcctggcg ggccagcgag
    16261 tccttggcgt agaggcgctg atagcgcgcc agggtgagct gggcgttggc cagctgcgcc
    16321 tggttctcgg cgagctggcc ctggtactgg gtgagctcgg cctggtaggg ccgcgtgtcg
    16381 atctgggcca gcaactggcc cttttccaca tactggccct cggtgaaata caccgcctgc
    16441 aagtggccgt cgatgcggct ggtgacggtg acgctggcgc tggcgctcac cgtgcccagc
    16501 gcggtgagcg ccacgggcac gtccgccgcc gtggcgctgg cggcctgcac cagggcggcg
    16561 gcgtccaggg ccggcctgct ccggaccccg cccaggcggg cgtgcagcag ataggcggcg
    16621 agcgccagga cgacgagcag gacggaagcg gcggcgagcc atcgcctgcg gcgcggggtc
    16681 ggggttgatg acgtgggatc catgatggcc tggaatggca gtgcgacaga tgactccagg
    16741 ccagaatgga aacaggaaaa tgtgcagaaa ctatggagat tgtgcttcgg gccttgtcga
    16801 tgccgggtgg atttcattta aatgacgaac ctccaaccgg ccgccccttc atgaaatcca
    16861 gcctgtccac ccgtttgttc cttgccatgc tggcgctgtg cgccctggcc atcctggcca
    16921 tgggttttgc ttcctacatc agctttaccc agggcttcct gggctacttg aacgaacagg
    16981 cggaggagcg catcgatgtc atcctgccgc gcttcgaaaa ggcctacgcc aaggcaggca
    17041 gctgggattt cctgcggggc cgtcccaatg tctggttctt catgacccgg ccccaggaag
    17101 ggccgccggg cgaagggccg ccggagccga acatctcgga cctgaccggc gccttcctgc
    17161 gcgtcacgct gttcgacgag atggacaact acgtgggcgg ctttcgcgga tataccaagg
    17221 aacagatcat gcgtcccgtg atgcatgacg ggcattccgt gggctgggtc gggataacgc
    17281 cgttcgagtc ggtgagcgcc gccggcggcc tccgcttcca ggacagccag ctgcgttcca
    17341 gcgtgctgat tggcatcgtc tgcctgctgc tggccatcgt ggtggcctgg cggatcagcc
    17401 tgggggtgct caagcccgtg cgcagcgtcg ccgaggcgac gcaccggttg gccgcgggcg
    17461 actattccat catcgcggtg cccgggcaga ggaacgacga agtggggcgg atggccgccg
    17521 atttcaaccg catggcgacc cagttggcca gcaacgagcg caagcggcgc gagttcatgg
    17581 ccgatatctc gcatgaattg cgcacgccgc tggccgttct gcgcggcgag ctcgaggcga
    17641 tggaggacgg catccggtcc gtctcgtccg acaccctcca gtccttgcag ggcgaggtgg
    17701 ccatgatgag caagctagtg gacgacttgt tcgaactgtc gctggccgag atcggcggtc
    17761 cgacctaccg ccgcgacccc gtcgacctga gcgatctgct ggcgcagtgc atggagtcct
    17821 tcctgccgcg gctggccgcg cgcaatatca gcagcctgct ggaggccccg ccgggcttgc
    17881 aggtccgcgg cgacgatcgc cggctgagcc agcttttctt caacctgctg gaaaacagcc
    17941 tgcgatatac cgaccccggc ggcacggtat ggtggcacgc caccctcgaa gaccagaacg
    18001 tccggatctt ttgcgaggac acggcgccgg gcgtgcccga atcgcagcag gagcgcctgt
    18061 tcgagcgctt ctaccgcgtg gaagcctcgc gcaaccgggc cagcggcggc gccgggctgg
    18121 ggctggccat ttgccggggg atcgtcgaag tccatggcgg cacgatcaag gccggcgatt
    18181 cgtcgctggg cggcctgtgc atcgaagtgc gcctgccggt gttgagaacg aaggccgcat
    18241 gaacgcgccg gcccgcatcc tcgtggtgga agaccaggcc aagctggccg cgttgatggt
    18301 cgattacctg cgcgccggcg gctacgcggt ggaccatgtg gccgacggca acctcgccgc
    18361 ccaggtcttg cgggaacagg tccacgacct gctggtgctg gacctgatgc tgccgggccg
    18421 ggacggcctg cggatatgcc gcgaattgcg cgactacagc cagatgccgg tgatcatggt
    18481 gacggcgcgg gtcgacgaga tcgaccggct gctgggcctc gagctggggg ccgacgacta
    18541 catctgcaag cccttcagcc cgcgcgaact ggtggcgcgg atcaaggcgg tgctgcgccg
    18601 ctacggtccc tggtccgagt cccggcggca ggccatgacg atcgatccgc aggcctacca
    18661 ggcccgctac aacggcgtgc tcctggacct caccccggtg gagtttcgcc tgctggccgc
    18721 gctgcatgcc ggctgcggcc gggtgctgtc gcgcgaccag ttgatgacgc acctctatga
    18781 agatcatcgg gtggtcaccg accgcaccgt ggatagccat gtgaagaact tgcgccgcaa
    18841 gctggaggcg gccatgcccg ggcaggatcc gatccattcc gtctacggcg tgggctattc
    18901 gctggatttg tcctgatgcc ggcactgttt gtcccggcat gtatataatt tcaaacagtt
    18961 acaggtgccc tggccgggtt ctcggccagg gttaaacggg aaagcggtgc gtccggcggg
    19021 gtttccgacc ctccaggcca agccgctgct gcccccgcaa cggtaagcaa gcgcgggcgc
    19081 gccacgaagc cactgggtca ctcctgggaa ggcggcgcgt caagacttgc gagcccggat
    19141 accggcctgc gacctccatc cacataactc ggcaagcggg gatgcttgtc aaagaggaat
    19201 catgtcatcc gtacgtctca aacaggccgc ggcgttctcg ccgctgctgc tgtccttcgt
    19261 tgttcccgcc cacgcccagc aaacgctcga tcccatcgtg gtgaccgcca cccgccagga
    19321 aacccgggcc tccgaactct tggccgatgt caccgtcatc gaccgcgccg agatcgagcg
    19381 ccatgccggc ggcaccatcg ccgatgtgct ggcgacgcag ccgggcatcc agttcgcccg
    19441 caacggcggg ccgggcacgt ccaccagctt ttatgtgcgc ggcgccaact ccgaccagac
    19501 caaggtgctg gtcgacggcg tgcccatcaa ttcgctggac gcatccgggt cgtcgctgcc
    19561 caatatctcg ctggccgatg tcgaccacat cgagatcgtg cgcggcccgg cctccacgct
    19621 gtacggcgcc gacgcggtcg gcggcgtcat ccagatcttc acccgcaagg gcgagcccgg
    19681 cgtgaaattc gacagcttca tcggctacgg cacgcaaaac accttgcagg ccaatgcggg
    19741 cctgtcggtc ggacaggaga aatggcgttt ccgcttcgag ggcaaccgca tgtccacgag
    19801 cggcatttcc gcgcagaccg gcgcgaccaa ccaggacgcc gacaaggatg gctacttcaa
    19861 caccggcggg gcggtgtcgt tcgccctgct gcccgcggaa ggccacgagc tgggcatgag
    19921 ctaccgccag aaccaggggc gcgcctacta tgacagcggc aacgtgccgg ccgacggcgc
    19981 cttcgacgac catgtcgact tcgtcacctc gcagtggcag ctctatgcca aggacaggct
    20041 gacctcgttc tggaacagca cgctgcaatt cggccagtcg ctcgactggc agaaaagcta
    20101 cggcgagtac gcgcccgagg gcacctttct gcagacggtc tcccagcagc tgagctggca
    20161 gaacgacatc acgctgccgc tgggcaaaat gctggtggcc tatgagcatt cggaccaaca
    20221 ggcctcggcc gacgacagcc agcctttcac ggcgggcgac tacatcacca acgactccgt
    20281 gctggtgggc tggacggccg attggggcaa gaaccgctgg caggtcaacg gccggcgcga
    20341 catcaactcg atctacggca gcaataacac gtattccgcg tcctacggct accagatcac
    20401 gcccgagtgg cgcgcgcagg ccagctacgg caccgcgttc aaggcgccca cgctctacca
    20461 gctctacacc ccgttctacg gcaaccccct gctcaagccc gagagctcgc gcaaccgcga
    20521 ggtgagcctg gtgtgggagc gcgacgagca gcgggcctcg ctgacctact accgcaacag
    20581 cgtcaccaac ctgatcgact gggtcctgat cgatccgtcc agcttcgccg gcgcgtatga
    20641 aaacgtggag caggcgaccc tgcagggcgt gacgctggcc tacagcggcc gtttcggcga
    20701 ttggcgcctg catggttcct acgactggct cgacgcggtg gacgacacga ccggcatgcg
    20761 gctgggccgc cgcgcgcgca acaagggcgt ggtgggcgtg tcccatatct gggggccggt
    20821 cgaggcgggc gccgaactgg tcgccgcggg cagccgcttc aacaccaatg accagacggg
    20881 cacgctgggc ggctacagcc tggtcaacct gacggccagt tatgctgtgt cgaagtcgct
    20941 ttccatccag gccagcgtca acaatgtatt cgacaagcac tacgagttgg cgcagggcta
    21001 cggcaccttg ggcatctcgg cgttcgtcgg catccgctac accatgcagt aagaggagac
    21061 aggcacggca tggaccttcc ttcggccttt gcgcgcaccg agctcgtcct gggcgggcaa
    21121 aagagcggca agtcccgcca tgcggaggaa cgggcgcgcg cctggctgcc ggcttgcggc
    21181 ggccaccggg ccgtgctggt cgccacgggc caggcccgtg acgccgaaat ggcggcgcgc
    21241 atcgcccggc accgccgcga tcgcgcgctg cgggtgcccg gcatggcgac ggtcgaagag
    21301 cccttgctgc tggcgcagaa aatagccgaa ctcagccgcc cggaggtttt ggtgatcgtc
    21361 gattgcatga cgctctggct gaccaattgg ctgatgcccg cggacggccc tgggcaggac
    21421 gagcccgcgc cgctgccggg gttcgacgcg caggcggccc tgcttttcgg cgcgctggcc
    21481 gaggcccgcg ggccggtcat cctcgtcagc aacgaaatcg gcctgggcgt gatacccatg
    21541 ggccggcacg tcagggcgtt cgtcgatgcg ctcggcaccc tgaaccagcg gctggcgcaa
    21601 gcgtgcgagc gcgtgacgct ggtatccgcg ggcctgcccc tggcgctgaa ggggcggccg
    21661 tgagacggct cgtgcttgcc ttgctgctgc tttgcggcgc gttgccggtc cacgcgctcg
    21721 agataacgga cgatcgcggg gtcgcggtcg tgctggacgg gccgccgcaa cgtatcgtca
    21781 gccttttgcc gtccctgtcc gaaacggtgt gcgggctcgg ccagtgccgg cggctggtcg
    21841 gggtggaccg ctattcttcc taccccgacg cattgaagca gctgccgcag gtgggcagcg
    21901 gcatggagcc caatatcgaa gccatcgtcg ccctgaagcc ggacgtggtc ttgatggcgg
    21961 tggcttcgcg ggtgagcgcc cggctcgaat cgctcggctt gaaggttgtc gcgctcgaca
    22021 cggaaaccta cgcggacgtg cagcgggtga cgcagacggt gggcgccttg ctgggcgtgg
    22081 atgacgcgcc gcgcgtctgg caggccatcg agctgggcgt tgccgacgcg gcgcggtcgc
    22141 tgccgccgca ggtcaggggc ctgcgcgtct atgacgaagt cggccgtctt tacgccgcag
    22201 gcgagtcgtc gttcatcggc gagaccctga agcggctggg cgtgcagaac atcgtgccgg
    22261 ccagcctggg gcctttcccc aaattgaacc ccgaatatgt cgtcaaggcc gatccggacc
    22321 tgatcatcgt cagccggaac gacgcgcccg acatgatgca gcggccgggc tggtccggca
    22381 tccgcgcggt gcgggaacac cgcatctgcg ccttgtctcc ggcgcagacc gacatcgtga
    22441 tccggcccgg gccgcgcctg cccgagggcg cgcgcatcct ggccgactgc atcgccggga
    22501 agggcctgtc ccaatgacgc gcacgcttcc cgcgcagcat cggcgcgcca ccctgtgggg
    22561 cggagggctt gccgttctgg cgctgtgcgc cggcgtgctg ggcatgggca tcggcagcac
    22621 gggactcgag aatgtctggc acctgcgcga cgaccccagc gcctggcgga tcgtctggga
    22681 gatacgcctg ccccgcaccc tgggcgcctg gctggcgggc gcgctgcttg gcttgtcggg
    22741 cgcgctcgcg caaggcctgt tccgcaatcc gctggcggat ccctacttgc tcggcagcgc
    22801 ctccggggcg tcgatgggcg tggcgctcgt gaccgctttc ggcgggattt ctcccctggg
    22861 agcgccctgg ctggcccggc tgggcctgac cggcgccgcc tttgtcgggg cgacctgcgc
    22921 ggtcctgctg acgctggccc tggcgcgcgg cgcgcaacaa accttgcggc tgctgctggc
    22981 gggggtgatc gtcggcgtcg tgctgggcgc ctgcaattcg ctcatgatgc tgcgctatcc
    23041 ctcggtcatg cagatcatgc aggccttcat cctgggcagc acggcattcg tcgactgggg
    23101 cgcgctgggc ctgatggcgg tcgcgtggat cgtctgcgcg ctggcggcgt ggagcctcag
    23161 ccgggtgctg gacggcttga cgctgggcga agagaccgcc gccagcctgg ggttgccgct
    23221 ggcccccctg cgcgcgatcc tgatcgccgt gctggccctg gcgatcggca cggcggtcgc
    23281 ccagaccggg ctcatcgcct tcgtggggct tgcctcgccg cacctggtgc ggtcgctggt
    23341 gaagaccacc cacaaccgcc ttatcctcct gtccagcctg atgggcggcg ccctgctgct
    23401 ggcggcggat ctgctggccc gctgggtgat cgcgccgcag gaactgccgg tcggcgtgct
    23461 caccgccctg atcggcggcg tgtatttgct gtggcggatg catgccggca atgtccgcgc
    23521 gggagggcta tgagtccgcc agccatcgcc gcgcgcgcgc tgggcgtgca actcggcggc
    23581 aggccgatcc tgcacggcat cgacctggcg ctcggacgcg gccgctggac cagcatcatc
    23641 ggtcccaacg gcgcgggcaa gtcgaccttg ctcaaggcgt tggccggcct gctgccccat
    23701 caaggcgacg tgctgctgcc tgggttgccg cccggcgagc agggcgggcg cagaaaggcg
    23761 cgccatctcg catggctggg ccagaacgag catggcgcgg acgatctcac ggtgtacgac
    23821 ctcgtcatgc tgggccgttt gccccaccag ccttggctga gcccgccgtc cgacgccgac
    23881 cgtgaggcgg tgcgggcggc cttggacatg atgcaggcct ggacgtggcg cgaccgcagc
    23941 cttggccagc tctccggcgg cgagcgccag cgggtgctgc tggcccgggc cctggccgtg
    24001 caggcggagc tcctgctcat ggacgagccg ctggccaatc tcgatccgcc gcaccaggtc
    24061 gactggctgc tgctggtccg cggcctggtc gcgcggggcg ggacggcggt cagcgtgctg
    24121 catgaaatcc cgctggccct gcaggccgac gacgtcgtcg tcatggcgca ggggcgcatc
    24181 cgctaccaag gccggtgcga tgcgcgcgcg acgcacgccg ccatcgaaca ggtgttcgac
    24241 aaccgcatac agattcggca agtcgacggg cagtggatct gccttccccg attttgaaag
    24301 aggagaccca gatgcaaatc gagcaacccc cgacagaaaa gccctacgag aaatccgagg
    24361 gcgaacgccg cggcctcgtc atcgtcaata ccggcgacgg caagggcaag agcacggcgg
    24421 ccttcggcct ggcgatgcgg gcgcacggcc gggacaaggc ggtcaagata ttccagttca
    24481 tgaaggtgcc gacggcgcgc ttcggcgaac accgcatctt cgagaaactg ggcctgccca
    24541 tcgaaggcct gggcgacggc ttcagctgga aaagcaagga cctggaccgc tcggcgcagc
    24601 tggcgcgcga cggctggcag cgcgcgcgcg cggcgatcct gggcggcgac tacttcatgg
    24661 tggtgctcga cgaggtcacc tatcccctgg tctatggctg gatcccgctg gccgacgtct
    24721 tgcagacctt gcgcgatcgt cccgccgcgg tgcatgtggt gctgaccggc cggcgctgcc
    24781 cgcaagagat catcgacctg gccgacacgg tgaccgagat ggccatggtc aagcacgctt
    24841 tcacgcaggg catacccgcc cagcggggca tcgaggactg atgaccttcc tgccggccgc
    24901 gatgccgtgg cgcgacggcg cgcatccgtg ggcattcgtc ctggcggtgg gcctggcatt
    24961 gctggtcgac atcgcgctgg gcgagccgcc cgccgcctgg catccggtgg tctggatggg
    25021 gcgctacctg gaggcggcgg gccggcggtt cgcgccggcg cgtgaaacgc cccgggggcg
    25081 accggacgtc cgggcctttg cccgggggac tttttattgg tgcgccgggg ctgcgctcac
    25141 cctggtcctg gccgcggtgc tgcaagcggt tctgcttgcc gctccagcct ggctcgccgt
    25201 ggccggcctg gccttgctgc tcaagcccat gctcgcctgg gccatgctgc gccgcgaggt
    25261 gcaagccgtg gagctggccc tggccgactc gctgccggcc ggacgcgaac gcctgtcgcg
    25321 gctttgcagc cgggatgtga gcgggctcag cgcggagcag gtgcgcgaga ccgccatcga
    25381 gagcctggcc gaaaacttga acgattccgt ggtggcgccg gtgttctggt tcgtcttgct
    25441 ggggttgccg ggggcggccg tgtaccgcta tgccaatacc gccgacgcga tgtggggtta
    25501 cctgggcatg cgcggcgggc gcgactggac gtgggccggc aagtgggccg cgcgcgccga
    25561 cgatgcgctg tcgtggttgc ccgccaggat cacggccgcc ttgctggcgc tggccgcggg
    25621 cggcatgccg gcgcgccgct tgcgccgcga ggccggccgc acgccgtcgc ccaacagcgg
    25681 ctggcccatg gccgcgatgg ccttggcgct gggcgtgcat ctgcgcaagc ccggcgtgta
    25741 tgtcttgaac caggacggcg ccgatccgca gcatgcggac acggcgcgcg cgatccggct
    25801 ggcggggcgg tgcgtgctgg ccttgccggt gctggccgcg ctggccgcct ggccgggcgg
    25861 gatggcggca tgatgcgcgt gcacggcggc cccgacgcgc tgggcgtgcc ccgttgggat
    25921 ttttcgacca acggcaatgc ctgcggaccg tgtccgcagg tcgtggcgca actgcaggca
    25981 gccgacatcc ggtcttatcc cgatccggcg taccaggcct tgcgccgcgc cctggcggat
    26041 tttcatgggg tcgaggcgtg gcgcatcgtg ccggccgcca gcgccagcga attcatcatg
    26101 cggataagcg cctgcctggc ccgcgcgggg caaggcaggg tgtggctgcc cgcgttggcg
    26161 tatggcgagt atgcccatgc cgcccggctc tggggcctgt cgcgggtgga cgagccggag
    26221 caggcggatc tttgctggct gtgcgagccg agcagccccc tggggggtga ggaaaagcag
    26281 ttgggcaagg tcgcgggaca ggactcggtc gtggtgctcg accgtgccta tgaaccgctg
    26341 cgactggctg gcaatagccc ggtgcgggcg caagaggccg gccgggtctg gcagctctgg
    26401 tcgcccaaca aggcgctggg catgaccggc atccggggcg cctatgcgat tgcgccgttg
    26461 tcgggcgcgg ccttggcgga ccggctggcg caactggcgc cgtcgtggtc cctgggcgcg
    26521 cacgcggtcg ccatgctggc ggcctggacc gcgccggcgg tgcaggcctg gctggcccgg
    26581 agccgcgaga cgctggccgg atggaaagcc gcgcagctcg atttgctcaa ggcctggcca
    26641 tgcctgccca gcgagacgaa tttcttttgc atgcgggccg cgctggatgc ggccttcctg
    26701 cgcgcccgcg gcatcaagct gcgcgacgcg gcgtcgttcg gcttgcctgg ccattggcgc
    26761 ctgagcgtgc agccgcccga ggcgcaggcg gccctgttcg ccgcgctcga ggcgggaggc
    26821 gccctgtgac ggcccgctgc gtcatggtgc tggggacgac cagcggcgcc ggcaagagct
    26881 ggctcgccac ggccttgtgc cgctggtacg cgcggcaggg gctgaaggtt gcgcccttca
    26941 aggcgcagaa catgagcaac aacgcgcggg tcgtcgccat gggcgaaatc ggctcggccc
    27001 agtatttcca ggccctggcc gccagggccg tgccggatgt ccgcatgaat cccctgctgc
    27061 tcaagcccga ggcggacacc cgcagccagg tcgtgctgct cggcaaggtg gatgaagccc
    27121 tgacccgatc gccgtggcgc cagcgcagcg aacaggtctg gccgcatatc gccgcgtcgc
    27181 tggatgccct gcgcgccgag aacgacgtgg tcgtcatcga gggcgcgggc tcgcccgccg
    27241 agatcaacct catggacagc gatatcgtca atatgcgcgt ggcgcgccac gcgcaggcgc
    27301 aatgcctgct ggtgaccgac atcgaccgcg gcggcgcgtt cgcgcacctg tatggcacct
    27361 gggccctgct gcctccgcaa gaccgcgccc tggtgcgcgg tttcgtgctc aacaaattcc
    27421 gcggcgatcc cgggctgctt gcgccggcgc cggcgcagat ccaggcattg accggcattc
    27481 cggtcgtggc ggtattgccc atgtggcgcg ggcacggcct gccggaagag gatggcgttt
    27541 tcgacgaccg cgccgttctt gcggggcagg ggcaggcggg gcggacggtt gccgtggtgg
    27601 cctatccgca cctcagcaat ctcgacgagt tcgaggcatt gaagcgggtg cccggcctgc
    27661 ggctgcgctg ggtgcgcagt ctggccgatc tggccgggat gggcgtggag gactggatcg
    27721 tcctgccggg gtccaagcac accagcagcg acctcgcctg gctgcgcgag caggacctgg
    27781 accagtttgt gctgcagcat gcgtattgcg gcggagccgt cctcggcatc tgcggcggct
    27841 tgcagatgct gggcgtgggc atggccgacc cgcatggcgt cgatggcgac gcgcgcgggc
    27901 tggggctgct gccgctgtcg accaccttcg ccctcgacaa gacggtgcgg cggtgttgcg
    27961 ccaggttcgg cgcggtggcc ggcgcctggg gggcgctgtc cggcctgccg ctgcgcggct
    28021 atgagatcca tcacggccag acccggatgg tccgggacgg cgcgcgcgcg gaggcggacg
    28081 gccgcgaagt catgcccggg ctggcgtggc aaaacgcggc gggcaatgtg ctgggcctgt
    28141 atgtgcacgg cctgttcgaa gacgcggccg tgctgcgcgc cctgctgggc gggcagggcc
    28201 cggacctcga tgccgtcttc gacggcctgg ccgattttct cgaacggcat gccgatcccg
    28261 gcttgctgta cgctctgatc ccttgacgct tggtagagat tccatgacct ttcttgttcc
    28321 cgccatcaac aatctcgaca accccgaacg ggccggacgg ctgcggcatg cgctcgacaa
    28381 caagaccaag cccgtcggct cgctgggccg tctcgaggcc ttggccgtgc ggctggggac
    28441 catcctgcaa agcgagtcgc ccgaattgcg cgccccgcag ctggtggtgt tcgcggcgga
    28501 ccatggcctg gccgcgcaag gcgtgtcggc ctatcccagc gacgtgacct ggcagatggt
    28561 ggaaaacttc ctggcgggcg gcgcggccgt cagcgtgctg gccaggcagc acggcatcgc
    28621 cttgaccgtg gtcgattgcg gcgtcaggca cgatttcgcg ccgcgcgcgg gcctgctggt
    28681 ccgcaaggtc gccgcgggca ccgccgatgc cctgcatgcc ccggccatga cggcggccca
    28741 gtgcgaacag gccctggaaa acggcaaggc cctgatggcc gatttgccgg gcaacgccct
    28801 gctgctcggc gaaatgggca taggcaatac ctcctgcgcg tcgctgctgc tgtcgcgcct
    28861 cgcgcaactc gacctggccg attgcacggg cgccggcacg ggcctggacg atgcgggcgt
    28921 cgccaggaag accgacatcc tgcgccgggt gctggccagg cacgacaacg cgcgccagcc
    28981 gctcgatgcc ctggcggcct tcggcggttt cgagatcgcc acgatggtgg gcgccatact
    29041 ccaggccgcc gcggaaaacc gggtgatcct ggtggatggc ttcatcgtca gcgccgcggt
    29101 attggtcgcc agccggctgg accccttggt attgcagcgt tgcgtcttcg cccatcgctc
    29161 gggggagcgg ggccatgccc tgatgctggc tgccttgcag ggcgatcccc tgctggatct
    29221 gcacttgcgc ctgggcgaag ggtccggcgc ggccctggcg tggccgctgc tgaattcggc
    29281 ctgcgtcatc ttgcgggaca tggccagttt cgagtccgcc ggcgtgtcgc gcgaggaaag
    29341 cgcgccttga gccctggcca tcgatgaacg ccctgcgtca tttactgctc gcgctgcagt
    29401 tcttcacgtg cattcccttg cccggaggcg tcgcgcgctg ggtcggcttc agtccggcca
    29461 tgcagcgtgc cagcttcgcc tattttccgt tggtgggcgc gctggtcggc gtggtggggg
    29521 cgggatgctt ccttggcgtg ctgcgctgcc tgccggcggt cggcgcatcg gcctggatcg
    29581 ccgccattct cggcagcgcg gcaacggcca tgctgaccgg ggcattccac gaagacggcc
    29641 tggccgacct ggccgacggc ctgggcggcc aggtcagccg ggagcgcgcc ctggaaatca
    29701 tgaaggattc gcgcatcggc agctacggct cggtcgccct ggtgctcgtt ctctcgctca
    29761 aggtgagcct ggtcgcggcg cttgcgcaag tcgatgcgcc gctcgctgcc tgggccttgt
    29821 tcgccggcca tgctttttcg cggctgatgc ccgtcggcgt ggcggcgata ttgcccaatg
    29881 cccgggaacc ggcggcgtcc aaatcgacgg ccgtgacggc gcgggtgggc tggctcgcgg
    29941 tgtccggcgc cgtcttctgg gccttgctgg cgctggctgt cgcggcctgg cgccagcccg
    30001 gccttttctg gatgggcggc atggcggctt gcggcgtggc atggctggcg atgtgccggc
    30061 tcctgcgcag gcgtttgcag gggtttaccg gcgatgcgct gggcgccacg cagcaggtct
    30121 gcgagctggc gttctatctg ggcctgctgt ttggcctgtc cgcggcaagg gggtagcgtg
    30181 aagctttggc tggtccgcca tgcaaggccc ttgatcgacc cgggcgtctg ctacggccgc
    30241 ctggacgtcg cggccgatcc ggcggcgacg caggcctgcg cgctatccct ggcggcggca
    30301 ttgccgcccg atttgcgcgt cctggcgtcg ggcttgcggc gggcgcgcca gcttgcccag
    30361 gcattgctgg ccttgcgcgc ggatttgcgg ctggccgtcg acgaacggct gggcgaaatg
    30421 gatttcgggc aatgggaagg cgtgccctgg cacgacatcc ccgaggacgc cgtcgatcgg
    30481 tgggcgcggg atttcgcccg gcatcgtttc ggcggcgtgg agagcgccgc cgacgtgctg
    30541 ggccgggtcg cccaggcgct ggccgccatg tccgcgctgg acgaggcggt ctggatcacc
    30601 catgcggggg tgatccgcgc cgtccatcat ctcgtccggc atggggacgg cgcggcgccg
    30661 acggcggcga cgtggccgtc ccaggcgccg gccttcggcg aatggacagt gcttacgatt
    30721 ggggagcaag catgaaactc ggcaagcgca gcgacgagat ctgttatccc ttcatctacg
    30781 aaacctcgca cctgtgcgac tacgagatcg tgaccgacga gctgtgcggc catatcggcg
    30841 cggccctgtc ttgcctggat gcgtcggagc cggcgatgcg cgacatcgcg caggacctgg
    30901 gccggctgca gcccctggct ttccacgcca atggttcgat acgcggccgg ctggccgtgg
    30961 acgaggcgga catcgcctgg ctgcagggcc gcttgtcgca ttaccgggat gaactggatg
    31021 gctgggtgca gggcttcgtc ctgccgcgcg gcctgccgcc ggtgccgcag ctgcatctgg
    31081 cgcgctcggc cgccaagaag gccatacgct gcctggtgcg ggtcgagcag gaagggcgcg
    31141 aggtgcccat ggtgctgccc aggctgtgca acctgatgtg caatgtcttc tttacgctga
    31201 ccctggtggt caaccggcgc cgcggctttg ccgagacgcc cttcaccagc aagagctacg
    31261 gttgaccgga gcaaggggcc ggaacccggg atttgttacg atccagggtt gatttccggt
    31321 gagcatcgcc gcgttggcgc gcagcctgcg cgcggcccag atacccagga ttgcccgtga
    31381 cctatcccaa gctgccttat cccctgtccg cctacaccca tggcgccgaa ttcgcgcgcc
    31441 agctgagccg gcgcatcctg atcctggacg gggcgatggg caccatgatc cagcgctaca
    31501 agctcaccga ggcggatttc cgcggcgagc gcttcaagga tcatgccaag gatctcaagg
    31561 gcgacaatga actgctgtcg ctggtgcgcc ccgacgtcat cgccgagatc cacaagcaat
    31621 acctggaagc cggcgccgac gtcatcgaga ccaatacctt cggcgccacc gccgtggccc
    31681 agtccgacta cgacctgccg gaactggcct atgaattgaa caaggcgtcc gcccagctgg
    31741 cccgcgccgc ctgcgacgcg ttcagcacgc ccgagcatcc gcgcttcgtg gcgggcgccc
    31801 tgggcccgca gcccaagacc gcgtccatct cgcccgacgt caacgacccg ggcgcgcgca
    31861 acgtcacctt cgacgagctg cgcgtggcct acgtcgagca gatgaacggc ctgctcgacg
    31921 gcggcatcga catcgtgctg atcgaaacca tcttcgacac gctcaacgcc aaggccgcca
    31981 tcttcgccgt ggaagaagtg ttcgaggcgc gcggcgtgcg cctgccggtg atgatctccg
    32041 gcaccgtcac cgacgcctcg ggccgcatcc tgtcgggcca gacggtcgag gccttctgga
    32101 actcggtgcg ccacgcgcgg ccggtcacca tcggcctgaa ctgcgccctg ggcgcggcgc
    32161 tgatgcgccc ttacgtggcc gagctgtcca agctgtgcga cacctacgtc tgcgtctatc
    32221 ccaacgccgg cctgcccaac cccatggccg agacgggttt cgacgaaggc ccttctgaca
    32281 ccgccggcct gctggaagag ttcgccaagg cgggactggt caacatggcc ggcggctgct
    32341 gcggcaccac gcccgaccac atccgcgcca tcgcgcagaa ggtgacggtg ctgaccccgc
    32401 gttctgttcc cgaggtgccg gtcaagaccc gcctttcggg cctggagccc gtcaacatcg
    32461 acgaggcctc gctcttcgtc aacgtgggtg agcgcaccaa cgtcacgggc agcaagatgt
    32521 tcgcgcgtct gatccgcgaa gagaaatacg acgaggccct ggccgtggcg cgccagcagg
    32581 tcgagaacgg cgcccaggtc atcgacatca atatggacga ggccatgctc gattcggccg
    32641 cgtgcatgaa gcgcttcctc aacctgatcg cttccgagcc cgacatcgcc cgcgtgccgg
    32701 tgatgatcga cagctccaag tgggacgtca tcgagaccgg cctcaagtgc gtgcagggca
    32761 agcccgtggt caactcgatc tccatgaagg aaggcgagga catcttccgc catcatgccc
    32821 gcctgtgccg ccgctacggc gccgccgtgg tggtgatggc cttcgaccag cagggccagg
    32881 ccgacacgct ggaacgccgc aagaccatct gcggccgggc ctacaagata ctggtggaag
    32941 aagagggctt cccgcccgaa gacatcatct tcgaccccaa cgtgttcgcg gtcgccaccg
    33001 gcatcgacga gcacaaccac tacgcggtgg acttcatcga aggcgcgcgc tggatacgcc
    33061 agaacctgcc gcatgcccgc atctcgggcg gcatctcgaa cgtgagcttc tcgttccgcg
    33121 gcaacgagcc catgcgcgag gccatccaca cggtgttcct gtactacgcc atccaggccg
    33181 ggctgaccat gggcatcgtc aacgccggcc agctgggcgt gtacgccgac ctgacgccgc
    33241 aattgcgcga cctggtcgag gatgtgatcc tcgaccgcgc cgagccggtg ggcaagaccg
    33301 aggccggcga cgagcgcacg cccaccgagc gcctggtgct gttcgccgac acggtcaagg
    33361 gggacggcaa aaagcgggaa gaagacctgg cctggcgcgc ggcggccgtg gaagaacggc
    33421 tcaagcacgc gctggtgctc ggcatcacca atttcatcgt cgaagacacc gaggaagtgc
    33481 gccagaagat cgaagcgcag ggcgggcggc ccatcgaggt gatcgagggg ccgctcatgg
    33541 acggcatgaa cgtggtgggc gacctgttcg gcgagggcaa ga
//
LOCUS       DASWPO010000325         8614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1774832, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000325 DASWPO010000000
VERSION     DASWPO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774832"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VGC69_17270"
     CDS             complement(<1..580)
                     /locus_tag="VGC69_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility-associated protein"
                     /protein_id="HEY0297085.1"
                     /translation="MLIVIGYVIVLGAVIGSFIVMGGHLGALFQPFEFTLIAGAAIGS
                     FLAGNSKKSISGVFKAIPATLKGSPYSKDVYMELMSLLYVILNKARREGLMAIESHIE
                     DPASSPIFAECPRILADAKLMEFTTDYLRIMISGNMSSFEIETLMDEEIETYRHEREV
                     PVSSLRSVADAMPAFGIVAAVLGVIKALAAVDQ"
     gene            complement(712..1287)
                     /gene="flhC"
                     /locus_tag="VGC69_17275"
     CDS             complement(712..1287)
                     /gene="flhC"
                     /locus_tag="VGC69_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEY0297086.1"
                     /translation="MASKSVSQEADEILLASAMITLGARLQVLEAETNLSHDRLARLY
                     REIRGCSPPKGMLPFSVDWFMTWLPNIHSSLFYNVYSFLNGRTKRKGIRAIIDAYRLY
                     LENAAADGGEANEPVLSFTRAWMLVRFFDSGMLQLSACRQCGGHFIAHAHDPQADFVC
                     AICRPPPRAGKTRAAKARGAQARAGLESARS"
     gene            complement(1312..1635)
                     /gene="flhD"
                     /locus_tag="VGC69_17280"
     CDS             complement(1312..1635)
                     /gene="flhD"
                     /locus_tag="VGC69_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEY0297087.1"
                     /translation="MKTADDSLLTDIREVNLSYLLLAQRMLRDDYAASTFRLGFSSEV
                     ADILLRLTPAQLIKLASSSSLLCRFRFDDYSLLSALTQDVLGGALQQAHATILLARQP
                     VEELG"
     gene            2008..2745
                     /locus_tag="VGC69_17285"
     CDS             2008..2745
                     /locus_tag="VGC69_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEY0297088.1"
                     /translation="MPSADDNLLHYTPLVRKLALQLLARLPASVQLDDLIQAGMIGLL
                     DAMRRYQESPEAQFETYATARIRGAMLDELRSQDWLPRSVRSKSRKIESAIQKQEQAR
                     MRPPSESEVAAELGVSLAEYRDMLNDAHGVQILHYEDFNRDGDQDGWHDAPASDQATP
                     LDALLAGDLRQALINAIGALPEREKLLMSLCYEQGLNLKEIGAILNVTEARVCQLRSQ
                     AIARIRAHLRSNAWQDLPPEMLLARVA"
     gene            2902..4599
                     /locus_tag="VGC69_17290"
     CDS             2902..4599
                     /locus_tag="VGC69_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY0297089.1"
                     /translation="MSMVINTNYLSLVAQNNLNTSQSALNTAIQRLSSGLRINSAADD
                     AAGEAISNRMTAQINGLDQASRNANDGISIAQTTEGALDSINDNLQRIRELTVQAANG
                     SNSASDLDSIQSEIQQRLAEIDRVSAQTQFNGVNVLAQNNTLSLQVGANDGQTISIDL
                     QQITTQTLGLNGFNVNGGGVVTNKAATAGDIIANGGSTNGGAYTYSATTQLKFVTAQQ
                     AFGQMQAGDTVKFATPAVNGGTSATYTMGADGNFTYSEVESAGSVSSLYSGQAESGTF
                     NNTNGANVSFTTDANGDVYIGGQKAYLTGGVLTTNNPSGLSQATLGGLLSAATGAGIS
                     VTVGATTYTGTGGNLSFTASVSEGQLESEFASTSGGTITLNRGLATASITFTGTTLGV
                     SNNTVIDNSGALTNDASDGLVANSTGATYSTTYTVDQNTGSVTVQSTTIDNTNSKYYA
                     ATGAAVYVDSSGRLTTASTSTGAATQDPLTALDTALATVDSMRSGLGAVQNRLESTIT
                     VLDNTNTNLADARSRIQDADYATEVSAMTKNQILQQAGTAVLAQANSQSQNVLSLLR"
     gene            4956..8570
                     /locus_tag="VGC69_17295"
     CDS             4956..8570
                     /locus_tag="VGC69_17295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0297090.1"
                     /translation="MRLHIRTDPAQAKKAAQQLTELRPDDYTGWLLLGQTEMQSDNFH
                     AAHKHYTHALELKENDSDVLTRLADVLFHLSQFEQAVERADQVLALKPNDVPTLIIKS
                     NSLNRMYRFDEAADVFVNQLIPLDPREPGIWNNLGTLKRDQGALAESEAAYIRSAQLA
                     KKAKRTDVLMQALSNRLTLLHYMPEKSADDILQAYKEWGVLFKSEGPVQRPRPTDLDP
                     RRKIRVGLYSEGFRQHPVGAMITPALEHLRELGIELYFYSASHVVDPLTQRLKAVADK
                     WTPIVNASDEHFAQVIRDDGIDIMMDLAGHNSGTRMRTMVSGPAPLLVKWVGGLINTT
                     GVQAIDYLITDHVESPLGSDAYYTEKLIRMPDDYICYLPPAHTPDVGPLPALKNGYMT
                     LGCFNNPTKINDVILEKWASLMRALPNSRLYLRSGAYGSEETRRHVFETMQKHGIGPE
                     RLRLEGQVNHRELLASYNDVDIALDPWPYSGGLTTCEAMLMGVPVVTLPGPTFAGRHS
                     ATHVANAGLPELVAQDWEQYQARVRELAANLDHLSTIRQRLRETLLKSPVCDGPRFAR
                     HLAVALRAIWQRYCADKTPAALVFTPEGKPWFEDEDAPMEVVQPDTPPPAPADHEFHF
                     AFQGKIVALDHGGAFTGSPASRGLSRLGSLTTIVIDPASNNLHNQDQFQREQLLQHYH
                     RHIALGDGESATLHTCLDRALSGTLEPLAAGKQLPMMRQSTTVLAQLSIPTTRLDAIN
                     GLQQLDWLILDNANDNKKILQGAEGLLPDMLIIHLQVLLIDIFKKQPDLGSLGKLLAK
                     YGFRLLRLDQPHHVSYLPQNTPLAKPPAKSQLMGIDAIFVPDDDRLKALDDNQRLKLA
                     FILHSAYKAPDFAYHVLQQTSDAVANRYLTASGWLKAPSKAAEPAIAASPKASVITAA
                     ALLRGKDVPQRAYVGVPVYNEERFIEQTVLSLKRQNADGVGFLISDNGSTDKTLELIR
                     DLAGDDERFQIFQQAQNVGALENFKFVLSHSHSEYFMWLGAHDYLSDNYLASAIACLD
                     DAPDIAMACGVPYGMLENSAPQPVQGAVYDFSAAAPLDRYMKSVAELANCTVLHALLR
                     RQLLDGFDIKRTISWDHVLISSLLWHGRLAFLPQARYFRRYFKARKENVEERIDPKAA
                     ALPRSDFYDYYAEDFARLAQPVLPAAEFRKHAKTVQAILQQRFEPAINVADPKTESKK
                     "
BASE COUNT         1822 a         2923 c         2401 g         1468 t
ORIGIN      
        1 gctggtccac cgcggccagc gccttgatca cgcccagtac cgccgccacg atgccgaagg
       61 ccggcatcgc gtcggccacg ctgcgcagcg aggacaccgg cacctcgcgc tcatggcgat
      121 aggtctcgat ttcttcgtcc atcagcgtct cgatctcgaa cgagctcatg ttgccgctga
      181 tcatgatgcg caggtagtcg gtggtgaatt ccatcaactt ggcatcggcc agaatgcgcg
      241 ggcactcggc gaaaatcggg ctggatgccg gatcttcgat gtgcgactcg atggccatca
      301 ggccctcgcg gcgagctttg ttgagaatga cgtacaggag cgacatgagc tccatgtaga
      361 catccttgct gtatggcgag cccttgagcg tggcggggat ggccttgaag acgccgctga
      421 tcgacttttt gctgttgccc gccagaaagg agccgatcgc cgcccctgcg atgagcgtga
      481 attcgaaagg ctggaacaga gcccccaaat ggccgcccat gacaatgaag ctgccgatga
      541 cagcgcccaa cacaatgaca taaccaataa cgatcaacac atcaggcctc tgctactagg
      601 agatattgat ccactggccc catgatcgcc tgccgcccac cttgcaaaaa ggcgggttac
      661 agggagtttt ggggggcatt tggcgtgttt gcgccaaagg cgcccgccga atcaggaacg
      721 cgccgattcc agcccggccc gggcttgcgc gccacgcgcc ttggcggcgc gggttttgcc
      781 tgcccgcggc ggcggacggc agattgcaca gacgaaatcc gcttgcggat cgtgcgcatg
      841 cgcaatgaaa tgcccgccgc attgccggca ggccgacagc tggagcatgc cgctgtcgaa
      901 aaatcgcacc agcatccagg cgcgcgtgaa actgagcacc ggctcgttgg cctcgccgcc
      961 atcggcggcg gcattctcga gatacaagcg gtacgcgtca atgatggcgc ggataccctt
     1021 gcgcttggta cggccgttca gaaacgaata cacgttatag aacagcgacg agtggatatt
     1081 gggcagccag gtcatgaacc agtcgaccga gaaaggcaac atgcccttcg gcggcgaaca
     1141 gccgcggatt tcccggtaca gccgcgccag acggtcgtgg ctgaggttgg tttcagcctc
     1201 gagaacctgc agacgagcgc ccagagtgat catggcgctg gccagcaaga tctcatcggc
     1261 ttcctgcgat acgcttttgc ttgccatacg aataaccgcg ccgtccgcga atcagccgag
     1321 ttcttcgaca ggctgcctgg ccagcaggat cgtggcgtgc gcctgctgca acgcgccgcc
     1381 cagaacatcc tgcgtcaggg ccgagagcag gctgtagtca tcgaaacgga aacggcacag
     1441 cagcgagctg gagctggcca gcttgatgag ctgggccggc gtcaggcgca gcaggatgtc
     1501 agcgacttcg ctgctgaacc ccaggcggaa ggtggatgcc gcgtagtcgt cgcgcagcat
     1561 gcgctgcgcc agcagcagat acgacagatt gacttcgcgg atatcggtaa gcaaggaatc
     1621 gtctgctgtt ttcacgcttt actcctcaac tgcaaaagta cggtggggaa ctcgaccggt
     1681 ctggtgcctc cccgtcatgc tcgcaccagg ctggtaatct actgtaatag atatgtatct
     1741 attcttagca aattaaggcc aatttatcac ggccattcgc aaaatttatt acattggtat
     1801 cactttacat accttgtctt tcgtttcagt tatcttctgc caccgaaaca acacttttgc
     1861 ttacattagg tgtggcaaat gatacagata aacgattaag aaagcactca aaacaagggg
     1921 tgcttgcccg aatgagccgc cccgactggc ccacaatgcc aggcacgacg acagcccacc
     1981 atgggcctat cgctatcgtg cgaccctatg ccttctgctg acgacaatct gcttcactac
     2041 acgccgctgg ttcgcaagct tgccctgcag ttgcttgccc gattgcccgc gagcgtgcag
     2101 ctggacgatc tcatccaggc cggcatgatc ggattgctcg acgccatgcg gcgctaccag
     2161 gaaagccccg aagcccagtt cgagacctat gccacggcgc gcatccgtgg cgccatgctg
     2221 gacgagttgc gcagccagga ctggctgccg cgcagcgtgc gcagcaagag ccgcaagata
     2281 gaaagcgcca tccagaagca ggaacaggcc cgcatgcgcc cgcccagcga gtcggaagtc
     2341 gccgcggaac tgggcgtgtc gctggccgag taccgcgaca tgctcaacga tgcccatggc
     2401 gtgcagatcc tgcactacga ggacttcaac cgggatggcg accaggacgg ctggcatgac
     2461 gccccggcca gcgaccaggc cacgcctctc gatgccctgc tggccggcga cctgcgccag
     2521 gccctcatca atgccatcgg cgccctgccc gaacgggaaa agctgctgat gtcgctttgt
     2581 tacgaacaag gcctcaacct gaaagaaatt ggggccatat taaatgtaac agaagcccgg
     2641 gtctgccagc tgcgcagcca ggccatcgcc cgcatccgcg cccatctgcg cagcaacgcc
     2701 tggcaggacc tgccgccgga aatgctgctg gcccgggtag cctgagactt tacattcctt
     2761 gcaatgacag aatgtgagcg acattcttaa gtttggacat aacgagtcgt aacccagggc
     2821 aacggagggc catccatccg ttagttgggc agcgcggctg cccagtttga aagaagtttt
     2881 tctctcttgg gagctcctac catgtcaatg gtgatcaaca ccaactacct gtctctggtt
     2941 gcgcaaaaca acctgaacac gtcgcaatcg gccctgaaca ccgccatcca gcgtctgtcc
     3001 tccggcctgc gcatcaacag cgccgctgac gatgccgccg gcgaagccat ctccaaccgc
     3061 atgactgccc agatcaacgg tctggaccaa gcctcgcgca acgccaacga cggcatctcg
     3121 atcgctcaaa cgaccgaagg cgcgctggac tccatcaacg acaacctgca gcgcatccgc
     3181 gaactgaccg ttcaagccgc caacggctcg aactcggctt cggacttgga ctcgatccag
     3241 tctgaaatcc agcagcgcct ggctgaaatc gaccgcgtgt cggcccagac ccagttcaac
     3301 ggcgtgaacg tcctggccca gaacaacacc ctgagcctgc aagtcggcgc caacgacggc
     3361 cagaccatca gcatcgacct gcaacagatc accacccaga cgctgggcct gaacggcttc
     3421 aacgtcaacg gcggcggcgt cgtgaccaac aaggcggcca cggccggcga catcatcgcc
     3481 aacggcggct cgaccaacgg cggcgcctac acctacagcg cgacgacgca gctcaagttc
     3541 gtcacggccc agcaggcttt cggccagatg caagccggcg acacggtcaa gttcgccacg
     3601 ccggccgtca acggcggcac ctcggccacc tacaccatgg gcgccgacgg caacttcacg
     3661 tactccgaag tcgaaagcgc gggctcggtt tcttcgctgt attcgggcca ggccgaatcc
     3721 ggcacgttca acaacaccaa tggcgccaac gtcagcttca ccaccgatgc caacggcgat
     3781 gtgtacatcg gcggccagaa ggcctacctg accggcggcg tgctgaccac caacaatcct
     3841 tcgggcctct cccaagccac gctgggcggc ctgctcagcg ccgcaaccgg cgccggcatc
     3901 tcggtgaccg ttggcgcgac gacctacacg ggcaccggcg gcaatctgtc gttcacggct
     3961 tcggtctccg aaggccagct cgagtccgaa ttcgccagca cctcgggggg caccatcacc
     4021 ctgaaccgcg gcctggccac ggcctcgatc accttcaccg gcacgacgct gggcgtttcg
     4081 aacaacaccg tcatcgacaa ctcgggcgcg ctgaccaacg acgccagcga tggcctggtt
     4141 gccaatagca cgggcgccac gtactcgacc acctacaccg ttgaccagaa caccggttcc
     4201 gtgacggtcc agtcgaccac catcgacaac accaactcca agtactacgc tgccacgggc
     4261 gcggccgtct atgtcgattc gagcggccga ctcaccaccg cctcgaccag cactggcgcc
     4321 gccacgcaag acccgctgac cgccctggac acggcgcttg ccacggtgga ctcgatgcgt
     4381 agcggtctcg gtgccgtcca gaaccgcctg gagtcgacca tcaccgtgct ggacaacacc
     4441 aacaccaacc tggccgacgc ccgctcgcgc atccaggacg ccgactacgc gaccgaagtc
     4501 tcggccatga ccaagaacca gatcctgcaa caggctggca ccgcggttct ggcccaggcc
     4561 aactcgcagt cgcagaacgt gctgtcgctg ctgcgttaag ccccccctgc acccagccag
     4621 gctccggcct ggcggcatgc caaaccccct ggccccgcca gggggttttt tattgggcac
     4681 gcccaccaaa cacgacacgg cgctcttggg agccaaccgg caaaactccc caggaaaagc
     4741 ggcagttttc ttttgtttac cggtcaattt caacagagcc ccactgggta gactgccaga
     4801 acatccccac accctatcct cctatggcca agcccacccg ccaactatcc attccccagc
     4861 ctgctgcttc caaagccccc cagacgctta ccttgcagca ggctcaaaac agggtgcaac
     4921 gcaatccatc ggatattttg gggtggcggg aattggtgcg gctgcacatc cggaccgacc
     4981 cggcccaggc caaaaaagcc gcgcaacagc tcactgagct gcgtcctgac gactacaccg
     5041 gctggttgtt gctaggacag acggaaatgc agtcggacaa tttccatgca gcgcacaagc
     5101 actacaccca tgccttggaa ctcaaggaaa acgacagcga cgtgctgacc cgcctggccg
     5161 atgtgctttt ccacctcagc cagttcgaac aggccgtgga gcgcgccgat caggtcctgg
     5221 cgttaaagcc caatgacgtg cccaccctga tcatcaagag caacagcctg aaccgcatgt
     5281 accgcttcga tgaggcggcc gatgtgttcg tgaatcaact catcccgctg gatcccaggg
     5341 aacccggcat ctggaacaac ctgggcacgc tgaaacgcga ccagggcgcc ttggccgaat
     5401 ccgaagcggc ctacatcagg tcggcccaat tggccaagaa ggccaagcga accgatgtcc
     5461 tgatgcaagc gctttccaac aggctgacgc tcttgcacta catgccggaa aaaagcgccg
     5521 acgacatcct gcaggcctat aaagagtggg gcgtcctgtt caagtctgaa ggccccgtgc
     5581 agcgcccgcg ccccacggat ctggacccgc gccggaaaat acgcgtgggc ctgtattcgg
     5641 aaggattccg gcagcatccc gtgggcgcca tgatcacccc ggcgctcgag catttgcgcg
     5701 agctcggcat cgaactttat ttttattcag catcccatgt ggtggatcca ctcacccagc
     5761 ggctgaaagc ggttgccgac aaatggacgc cgatcgtcaa cgccagcgac gagcatttcg
     5821 cgcaggttat ccgcgacgac ggcatcgaca tcatgatgga cctggccggc cacaacagcg
     5881 gcacccgcat gcgcaccatg gtatcgggtc ctgcgccgct gctggtgaag tgggtgggag
     5941 gcctgatcaa taccaccggc gtgcaagcca tcgactacct gattaccgat cacgtcgaat
     6001 cgcccctggg cagcgatgcc tactacacgg aaaagttgat ccgcatgccg gacgactaca
     6061 tctgctatct ccctcctgcg cacacgccgg acgtcggccc tttgcccgcg ctgaaaaacg
     6121 gctacatgac gctgggctgt ttcaacaacc ccaccaagat caacgacgtc atcctggaaa
     6181 aatgggcgag cctgatgcgc gccctgccga attcccgcct ttacctgcgc agcggcgcct
     6241 acgggagcga ggaaacgcgc cggcatgtgt tcgagaccat gcaaaagcac ggcataggtc
     6301 ccgagcgcct gcgcctggaa ggccaggtca accaccgtga actgctggcc tcctacaacg
     6361 acgtggacat cgcgctggac ccctggccct attccggcgg cctgaccacc tgcgaggcca
     6421 tgctgatggg cgtgcccgtg gtcaccctgc ccggccctac ctttgccggc cggcactccg
     6481 ccacgcacgt ggccaacgcc ggccttcccg aactggtcgc ccaggattgg gagcaatacc
     6541 aggcgcgggt gcgggagctg gcggccaacc tcgaccatct ctccaccatc cgccagcgcc
     6601 tgcgcgaaac cctgctgaaa tcaccggtct gcgacggtcc gcgcttcgcc aggcacctgg
     6661 cggttgccct gcgcgccatc tggcagcgct attgtgcgga caagacgccc gcggcgctgg
     6721 tgttcacgcc cgaaggcaag ccctggttcg aggacgagga cgcgcccatg gaggtggtcc
     6781 agcccgacac accgcctccc gctcccgccg accatgagtt ccactttgca ttccagggca
     6841 agatcgtcgc gctggatcac ggtggcgcct ttaccggcag cccggcctcc cgcggcctga
     6901 gccgcctggg cagcctcact accatcgtca tcgatcctgc cagcaacaac ttgcacaacc
     6961 aggaccagtt tcagcgagaa caactgctgc agcactatca ccgccacatc gcgctgggcg
     7021 acggtgaatc cgccacgctc catacctgtc tggaccgtgc gctcagcggc acactggaac
     7081 ccctggccgc gggcaaacaa ttgcctatga tgcggcagtc caccacggtg ctggcccaat
     7141 tgtccatccc caccacccgg ctggatgcca tcaacggctt gcaacagctg gactggctca
     7201 tcctggacaa cgccaacgac aacaagaaga tcctgcaagg cgcggaaggt ctgctgcccg
     7261 acatgctgat catccatctg caagtcttgc tgatcgacat cttcaaaaag cagccggacc
     7321 tgggcagcct cggcaaactg ttggccaagt atggattccg gcttctgcgc ctggatcagc
     7381 cgcatcatgt cagctacctg ccgcaaaata cccccctggc caaaccgccg gcaaaatcgc
     7441 aattgatggg gatcgatgcg atcttcgtcc ccgacgacga ccgcctcaaa gcgctggacg
     7501 acaatcaacg cctgaaactg gccttcatcc tgcattcggc ctacaaagcc cccgactttg
     7561 cctatcatgt gctgcagcaa accagcgatg ccgtggcgaa ccgctacctg acggcaagcg
     7621 gctggctgaa agccccctcc aaagcggccg aaccggccat cgccgccagc cccaaggcat
     7681 cggtcattac cgcggctgcc ctgctgcgcg gcaaggacgt gccgcaacgg gcctatgtcg
     7741 gcgtcccggt ctataacgaa gagcgattca tcgagcagac cgtgctgtcg ctgaaacgcc
     7801 agaatgccga cggcgtcggc ttcctgatct cggacaatgg ttcgacggac aagacgctgg
     7861 aactcatccg cgacctggcc ggcgacgacg agcgcttcca gattttccag caggcgcaga
     7921 acgtcggcgc cctggaaaat ttcaagttcg tgctgtcgca cagccactcc gagtacttca
     7981 tgtggctggg cgcccacgat tatctttccg acaactacct agcatcggcc atcgcctgcc
     8041 tggacgacgc gcccgacatc gccatggcct gcggcgtacc ctacggcatg ctggaaaaca
     8101 gcgcgccgca gccggtccaa ggcgccgtct acgacttctc ggccgcggcg cccctcgatc
     8161 gctacatgaa atcggtggcc gagctggcca attgcacggt gctgcacgca ttgttgcgcc
     8221 gccagctcct ggacggcttc gacatcaagc gcaccatttc ctgggaccat gtcctgatca
     8281 gcagcctgct gtggcacggc cgcctggcgt tcctgcccca ggcccgctat ttccggcgct
     8341 acttcaaggc ccgcaaggaa aacgtcgaag aacgcatcga ccccaaggcc gccgccctgc
     8401 cccgctcgga cttctacgat tactacgccg aggattttgc ccgcctcgcc cagcctgtcc
     8461 tgccggcagc cgaattccgc aagcatgcca agaccgtgca agccatcttg cagcagcgct
     8521 tcgagcccgc aatcaacgtc gccgatccga aaaccgaaag caaaaaatga aagccgtgat
     8581 tcttgccggt ggcctcggaa cccggatttc cgaa
//
LOCUS       DASWPO010000326         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1327963, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000326 DASWPO010000000
VERSION     DASWPO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327963"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VGC69_17300"
     CDS             <1..338
                     /locus_tag="VGC69_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein MotA"
                     /protein_id="HEY0297091.1"
                     /translation="LAVALMSTFYGLLLANLICKPIAVKLERRTEQRIVLMNMVLQGI
                     AMMCERRGPALVRETLNSFMLQVEDEIYDGGQPQAAAPARAAPGLPGAAPRPGIPPAA
                     APLKATGRP"
     gene            335..1330
                     /locus_tag="VGC69_17305"
     CDS             335..1330
                     /locus_tag="VGC69_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0297092.1"
                     /translation="MSDGETIIQERRRPSLADLVDQRKREARRHRSLAPTRLNWLDEG
                     RFSRWHVPESAEQEADNWLLTYLDMLTLLLAMLVVMLGISRLPPVHKPGDPPMTLVGS
                     VARGGLPAYAGPIAFDNTPLVPASWADIPLPGELPPANPADVGPLPRVAQAPAPLVPP
                     SIKDLGLSDLGKGVEVVINSQSISFRISNELLFPSGQATLNPDGLAVLKKLAVVVNRS
                     KYPLSVEGHSDNIPIQTPQFPSNWELSASRATSVLRELVRDGVANDRLRAVGYADTRP
                     LKPNDTLQGRAANRRVELILQITPRRPDAPDAEPSVPMPASRVLPTGAKPAVSKP"
     gene            1340..>1999
                     /locus_tag="VGC69_17310"
     CDS             1340..>1999
                     /locus_tag="VGC69_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297093.1"
                     /translation="MIRTKFDFRGQSFRSVLGYTWQHWQRQPWRIAVIAASALGSAMA
                     DVFTPLYAGRLIDELTSGNAKEIVWPAALHAFWLLIGLGLAGILLRQAAYMNIISLTL
                     KMMYDIAASSFYKVQRFSTDWHANSFAGSTVRKITRGIWALDLLDDTLLVALLPSLAM
                     LVGATVLLALHWPVMGLVVGLGALIYIVATGLLALGYVAPSARLANSWDTRLGGALAD
                     AV"
BASE COUNT          300 a          762 c          634 g          303 t
ORIGIN      
        1 acctggccgt ggcgctcatg tccaccttct acgggctgct gctggccaac ctgatctgca
       61 agcccatcgc cgtcaagctc gagcgccgca ccgagcagcg catcgtgctg atgaacatgg
      121 tgctgcaagg catcgccatg atgtgcgagc ggcgcggccc ggccctggtg cgcgagaccc
      181 tcaactcctt catgctgcag gtcgaggacg aaatctatga cggcgggcag ccgcaggccg
      241 ccgcgcccgc gcgcgccgca cccggcctgc ccggcgccgc tccccgtccc ggcatcccgc
      301 ccgccgccgc gcccctcaag gccacgggcc ggccatgagc gatggcgaga ccatcatcca
      361 ggagcgccgc cggccctcgc tggccgacct ggtcgaccag cgcaagcgcg aggccaggcg
      421 gcatcgcagc ctggccccca cccgcctgaa ctggctggac gaaggccgct tctcgcgctg
      481 gcacgtgcct gaaagcgccg aacaggaagc cgacaactgg ctgctcacct acctggacat
      541 gctcaccctg ctgctggcca tgctggtggt gatgctgggc atctcgcgcc tgccccccgt
      601 ccacaagccc ggcgatccgc ccatgaccct ggtcggctcg gtcgcccgcg gcggcctgcc
      661 cgcctatgcc ggcccgatcg cgttcgacaa tacgccgctg gtccccgcca gttgggcgga
      721 catccccctg cccggcgagc tgccgcccgc caacccggcc gatgtcggcc cgctgccgcg
      781 cgtggcgcag gcccccgcgc ccctggtgcc gccctccatc aaggatctgg gcctgagcga
      841 cctgggcaag ggcgtggaag tggtgatcaa cagccagtcg atcagctttc gcatcagcaa
      901 cgaactgctc tttccctccg gccaggccac gctcaacccc gacggcctgg ccgtgctgaa
      961 gaaactggcc gtggtggtca accgcagcaa gtaccccttg tccgtggaag gccacagcga
     1021 caacatcccc atccagacgc cgcagttccc gtcgaactgg gaactgtccg ccagccgcgc
     1081 caccagcgtg ctgcgcgaac tggtgcgcga cggcgtggcc aacgaccgcc tgcgcgccgt
     1141 gggctacgcc gatacgcgtc cgctcaagcc caacgacacc ctccagggcc gcgcggccaa
     1201 ccggcgcgtg gagctgatcc tgcagatcac gccccggcgc cccgacgcgc cggatgccga
     1261 gccctcagta cccatgcctg cgtcgcgggt actgcccacc ggcgccaaac ctgccgtctc
     1321 caagccctaa aatgggcgga tgatacgcac caaattcgac ttccgcgggc agtccttccg
     1381 cagcgtgctg ggctatacct ggcagcactg gcagcgccag ccctggcgca tcgccgtgat
     1441 cgccgcctcg gcgctgggtt cggccatggc cgacgtgttc acccccctgt acgccggccg
     1501 cctgatcgac gagctgacct cgggcaacgc caaggaaatc gtctggccgg ccgccctgca
     1561 cgccttctgg ctgctgatcg gcctgggact ggccggcata ttgctgcgcc aggcggccta
     1621 tatgaacatc atcagcctca cgctgaagat gatgtacgac atcgccgcga gcagcttcta
     1681 caaggtgcag cgtttttcca cggactggca tgccaacagc tttgccgggt cgaccgtgcg
     1741 caagatcacg cgcggcatct gggcgctgga cctgctcgac gacacgctgc tggtggccct
     1801 gctgccctcg ctggccatgc tggtcggcgc cacggtgctg ctggccctgc actggccggt
     1861 gatggggctg gtggtggggc tgggcgccct gatctacatc gtcgcgaccg gcctgctggc
     1921 cctgggctac gtggcgccgt cggcgcgcct ggccaatagc tgggacacgc gcctgggcgg
     1981 cgcgctggcc gacgcggtc
//
LOCUS       DASWPO010000327        18209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3023144, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000327 DASWPO010000000
VERSION     DASWPO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 18209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18209
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023144"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VGC69_17315"
     CDS             <1..485
                     /locus_tag="VGC69_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginyl-transferase family protein"
                     /protein_id="HEY0297094.1"
                     /translation="TADYAPNRSQRRAWKQHQPLRAFVAELAWSPEHYALYTRYQQGR
                     HPGGGMDEDSRTQYAQFLLTTRVNTRLVEFRTPEGVLTMVSIIDVLDDGLSSVYTFYD
                     PDMPGSLGTYGIMWQIEQCRLLNLPWLYLGYWIAESRKMAYKSGFQPLQKLIDGAWQQ
                     "
     gene            659..1702
                     /locus_tag="VGC69_17320"
     CDS             659..1702
                     /locus_tag="VGC69_17320"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY0297095.1"
                     /translation="MSILFNAYPLARAALFAMDAETAHEVTLANLQRAYDCGATRALL
                     HANPKAPATLMGLKLANPVGLAAGLDKNGAYIDALGNLGFGFIEVGTVTPRAQPGNPK
                     PRMFRLPKADALINRLGFNNQGLDAFLANVQRSQWREQGGVLGLNIGKNADTPIERAA
                     DDYLLGLAGVYPHADYITVNISSPNTKNLRALQGEDELSALLAQLRDKRAELADRHGR
                     QVPLVVKIAPDLAQEQVDAIADILPRHGIEGVIATNTTLSRTAVEGLPHAQEAGGLSG
                     APVHELSLAVIERLREQAGRSLAIIGVGGILSGKQAREKIAAGADAVQLYTGLIYRGP
                     ALVAECARALAVR"
     gene            complement(1757..2023)
                     /locus_tag="VGC69_17325"
     CDS             complement(1757..2023)
                     /locus_tag="VGC69_17325"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0297096.1"
                     /translation="MSKSKFKPMPSLKSDTQAEHFVETADLSEYDLSGFKPMRFEVEP
                     KSAALNMRLPQSLLDAVKAKAKAAGVPYTRYVRLVLEQAVANGG"
     gene            complement(2444..3019)
                     /gene="phaP"
                     /locus_tag="VGC69_17330"
     CDS             complement(2444..3019)
                     /gene="phaP"
                     /locus_tag="VGC69_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039187.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEY0297097.1"
                     /translation="MSAIPQQVLARQKAAVGTLIAAQTAVFEGFEKLVDLNLKVVKAT
                     LDEAAQKSHQAAELKDAQDASSFVSAALQPGADKAMAYGKHVYDIFAGVQAELAKLAE
                     AQIAEGREQVNAAVEQITKNAPAGSESAVAMLKSSLATANSAYESLTKAAKQAAEVAE
                     TNLNAAANATFKAATDVAEAAGKATTRRRAA"
     gene            3426..4412
                     /locus_tag="VGC69_17335"
     CDS             3426..4412
                     /locus_tag="VGC69_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HEY0297098.1"
                     /translation="MNRMTRTLAATLGAGALALSATAAQAADFPDHTIDFVVPFAAGG
                     PTDTVARALAQAMSASLNGQGIVVENKGGAGGTIGTSFVARAQPDGYTLLLMHVGFST
                     APGLYKNPGYDPYTSFEPIGLVVDVPMTVLARSDFPPNTLQELATYVKQNASKITLAN
                     AGIGAASHLCGTMLTSALGVNLLTVPYKGTAPAMNDLLGKQVDMLCDQTTNTTQQINA
                     KTVKAYAVTSLTRVPTLPDLPTMDESGYKGFQVGIWHGLWAPKGTPKPIVDKLVKALQ
                     AGLADPKFQANMKQLGTTVLTSEANPAALDAKVKQQVPQWTELFKKVGVEKQ"
     gene            complement(4467..5068)
                     /locus_tag="VGC69_17340"
                     /pseudo
     CDS             complement(4467..5068)
                     /locus_tag="VGC69_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(5078..5728)
                     /locus_tag="VGC69_17345"
     CDS             complement(5078..5728)
                     /locus_tag="VGC69_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0297099.1"
                     /translation="MIPFTFWDVFRNLLLSIPWTLLLSAEAFLGGAVVGVLLLVLRLA
                     RPRAFGLPIAAYVQVFQGTPLLMQLFLVYFGLALVGVETTPMTAASICVVLYASAYFT
                     ETWRGCVESVPRGQWEASASLALTFTEQLRHVIFPQAIRLSIPPTIGLMVQIIKNTSL
                     ASVVGFVELTRSGQMIANVTFQPFLVYGLVAFFYFALCFPCSLFGAWMERRLRVRH"
     gene            complement(5725..6399)
                     /locus_tag="VGC69_17350"
     CDS             complement(5725..6399)
                     /locus_tag="VGC69_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0297100.1"
                     /translation="MLNFSSVLAAWPAFLGGLAMTVLLTFFSCCCGTLLGIACAWCRV
                     YGARRLRLAVGCYVEFFRNTPFIIQIFFIYFGLPAIGLKFASFPAAILALTVNLAAYG
                     CEIIRSGIEATPRGQIEAAESLGLGRWQSFTRVVLPPAMSRVWPGMTSQLVIIMLATA
                     VCSLISTEELSYVTNRIAAETFRQFEAYIIVTILYLLLSICFRRFLHWLGPRFIFGCS
                     PVGAGR"
     gene            complement(6447..7163)
                     /locus_tag="VGC69_17355"
     CDS             complement(6447..7163)
                     /locus_tag="VGC69_17355"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297101.1"
                     /translation="MDSHASIASERLARWACQLSDDQVPDPVRRRRYACRTSRARARS
                     WAWGAAIPPVCLSSHAAADALGELMARYRFSADDVVRIVCDVPDIVVANLRYGVPDTP
                     EQARFSMPFAMAATLQRPDWGLAAMAPHRLAQDGLRGLMRRVDMVTGPHWNDADVRRR
                     APEGAQVRVALRDGRVLQASKDKAVGNPREPLGESRIARKFLDCAMPAVGAGRAERLL
                     ETLHGLDSALAIQTLFDGLA"
     gene            complement(7236..8021)
                     /locus_tag="VGC69_17360"
     CDS             complement(7236..8021)
                     /locus_tag="VGC69_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0297102.1"
                     /translation="MKIKKWIIGAASVLSCIAALPAQAADLLDEITSRGTLRVAVPTD
                     YPPFGFVGPDMKAQGLDVEMANLIGQKLGVKVELVPVTAPNRVPYLQTGKTDLTISSL
                     GKTAERAQVIDFSIAYAPFFDAIFGAKGSTAKTYDELSSKEIAVTRGSMQDQELKDLA
                     PKAVVKRYEDNSSTISAFLSGQTEFFASGTPVAAALTQRNPKLDMELKVVLANSPCYI
                     GVRKDQPKLLAKVNDIIRESKKNGTLDRMSLHWMGAPAGDLPE"
     gene            complement(8158..8571)
                     /locus_tag="VGC69_17365"
     CDS             complement(8158..8571)
                     /locus_tag="VGC69_17365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0297103.1"
                     /translation="MAHTRIRKFNTRETYPEQRIDNDLCQAVVARGTMVFLRGQIGQN
                     LDTSACVAIGDVAGQAEQAMKNIDMLLKEAGSKLDHICKVVIYISDPRYREDVYNVMG
                     KWLKGVFPVSTGLVISAFARTEYLVEIDVTAVIPD"
     gene            complement(8610..9581)
                     /locus_tag="VGC69_17370"
     CDS             complement(8610..9581)
                     /locus_tag="VGC69_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY0297104.1"
                     /translation="MANPPLTVPPKTGHKTLLYSELVTEMEGLNSDIAVLGMPFGSPY
                     APQAFSNDQTRAPQAIRDVTDRMVRAPEHYDFDIDGPLLQGRADIRFVDCGDVIPDLG
                     IPGGHLRRCEEAIRHILRGGGMPIVLGGDHGITNPVLRAYEALHEEITLVHVDAHLDW
                     RDEVNGIRDGLSSPIRRASELPHVGKIIQIGLRAQGSGRPADYEAAKAYGAELITAYE
                     LHDVGMQAVLDRIPDGGNYYLSIDADGMDATVMPAVDGPAPGGVTFVQARKLIHGLVA
                     KGRVVGMDIVEIQPSKDTATKLTCVTAGRLIVNLIGSSIRAGYFDRA"
     gene            9734..10642
                     /locus_tag="VGC69_17375"
     CDS             9734..10642
                     /locus_tag="VGC69_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0297105.1"
                     /translation="MLKLDFDERDLRSLRVFCHVAEAGGFAAAEQRLLMSKASISRHV
                     RDVETRLGVRLCERGPGGFRLTAEGAVALNLATTALRALSRIRPEIDAAHGVLSGPLT
                     IGLGEHTLTHPTCRLPEALSALQQQAPHVQPEIVVMSFKDLDQALRTQRIDIAIRGKY
                     SEDRDFSYLPLYIETHRIYVPSRVTRAKDQARLPLVYRPHPYVDRALATGRYQRGPEA
                     SGLDGVGALVATGYYQGLLPTHYGELLQERFGLRQAGRGETYSHAGCAVMLASRQLSQ
                     RAELFLRILRALHPIKGKALPASVRA"
     gene            complement(10685..11536)
                     /locus_tag="VGC69_17380"
     CDS             complement(10685..11536)
                     /locus_tag="VGC69_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0297106.1"
                     /translation="MRDMPASSPDSPTPAAQPDADSKSGIRSSEVAIQVIDRAMRLLD
                     ALAAQPDPVTLKELSATTGLHASTAHRILNDLVVGRYVERVDNGLYQLGMRLLELGSL
                     VKGRLNVREAAQSAMRTLHRQTGQTINLSVQQGDEIVYIDRAWSERSGMQVVRAIGGR
                     APLHLTSTGKLFLSIADARQVRAYAQRTGLSGHTRNSLTDIDRLQRELTEVRRHGYAR
                     DNEELELGVRCIAAGIQDDTGKLVAGLSISAPAERMRDEWIDLLIHTAAGISEALGYE
                     AGVSAAR"
     gene            complement(11603..12403)
                     /locus_tag="VGC69_17385"
     CDS             complement(11603..12403)
                     /locus_tag="VGC69_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY0297107.1"
                     /translation="MATSSRRIGLIIVGDEILSGRRQDKHFSKVVEMLGARGLQLAWA
                     EILGDERDQLVAAYQRSFASGDIVFSCGGIGGTPDDHTRQAAAAALGLPLELHPEARE
                     AITQRTIEMAQKEGRPAPDMSTPEHQQRLQMGVFPRGCEIVPNPYNRIPGFFIQDHTF
                     VPGFPVMAWPMLEWTLDTRYRALHHVEQRAEHAFLIYGLPESRITPAMETVEQRWPGV
                     RAFSLPSVGEDGNRPHIELGVKGEPGPAAEALAFLHAEVERLGGRLTP"
     gene            complement(12422..13207)
                     /locus_tag="VGC69_17390"
     CDS             complement(12422..13207)
                     /locus_tag="VGC69_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEY0297108.1"
                     /translation="MSYPSRNVSRPYGQSAAVARAFKRALVSQCHPAMLFAVLLPFLI
                     ALVGAILLLWLFWMPLTDWLRMEFARWGAIDRIDGWLTTVGLFSLKLYLTPVIAAAIL
                     LPVSGILGLAIAAVFVMPLVLGHLGRGEYAGLARQGRHVAAVSVWNALWVSAVFAVGW
                     LLTLPLWLVPPMAVLLHVFWWAFAFSRMLRVDAIADHASPAERRLLLKRHNMSFWGIG
                     LVCSLINLLPPAWVVLPVYSSLVYAHYGLDALARLRGERTIDV"
     gene            13280..13663
                     /locus_tag="VGC69_17395"
     CDS             13280..13663
                     /locus_tag="VGC69_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0297109.1"
                     /translation="MSLFVPGTDDAALRQALAAPGTWLVACFCAAWCNTCTEYRPKLQ
                     ALSEARPQHAYAWIDIEDEPDLLGDEDVENFPTLLIQAGGRVLFYGPMLPHIGHLERL
                     LDNLGMDSPAVSTQLPDVAQRLAQR"
     gene            complement(13664..15124)
                     /locus_tag="VGC69_17400"
     CDS             complement(13664..15124)
                     /locus_tag="VGC69_17400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0297110.1"
                     /translation="MSQPIPIDTPAADVPAAEAASRTFAEFDLHPLLLKSIADTGYTT
                     PTPIQAQAVPVAMQGRDVMGAAQTGTGKTAAFTLPILHRLMPMANTSASPARHPVRAL
                     ILTPTRELADQVYENVKRYTKHTPIRSAVVFGGVDIGPQKEALRAGCEVLVATPGRLL
                     DHVEQKNVNLGQVGILVLDEADRMLDMGFLPDLDRIIRLLPAQRQGLLFSATFSNEIR
                     KLGRTFLNDPVEIEVAARNATADTVTQVAYAMPGEAKRAAVVHLVKSRGLKQVIVFSN
                     TKIGTARLARLLERDGVKAESIHGDKSQAERMKALEAFKAGELEVLVATDVAARGLDV
                     AGVPCVINYDLPHSAEDYVHRIGRTGRAGASGEAIALYSPDEERYLLDIEKLIKRQVP
                     RGKLDLPAEASSRASGERERGDRGERSPRVERGERRERSPHDHRAPRAAPVDEFFLKP
                     YEPSQDPSAESEASKPASTAGKRPLAALLGGGRKAS"
     gene            complement(15304..17589)
                     /locus_tag="VGC69_17405"
     CDS             complement(15304..17589)
                     /locus_tag="VGC69_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEY0297111.1"
                     /translation="MTFNASKRRFLHSSLGMGAAATVLSTFPPAIRRALAIEANNATG
                     TINDVQHVVLLMLENRSFDSYFGTYKGARGYGDRFPVPAPGTPNIFYQTYTGAAAPGA
                     GTLIPYHLDETAGNAIRAGGTPHTWVDAQAAWDSGRMSNWPDSKTPLSMGYYEAAEVP
                     FQRSLAEAFTLCDHYHCSMHTGTIANRLFYWTGSNGPNGTDPNGGAYTPTAGLNNEFN
                     GGNDIGPSTTGWTWTTYADRLEKAGVKWKVYQSLIDNYGCNEMMGFRHWRKAIEDMPA
                     GRRPIYVATENIQTQPATAAGPFYDPAIDDALSPLAKGFGNTMPYGFLETFRDDIQNG
                     NLPAVSWIIPPSIYSEHPGPSSPAQGGWYVQEVLDALTANPEVWSKTVLLINFDENDG
                     FFDHLPPPTAPAHNPDGTLAGGQTLSDSDMAVEYHNYAPIDGTQPAVDGRPFGPGPRV
                     PMWVVSPWSRGGFVDSQVFDHTSTLMFLEKRFGVKEPQISNYRRAICGDLTSCFNFVN
                     PNSAPLATLTGRTDKVTADNLAASQAALGAVPKSTATPGSPLPVQAAGTLPSRALPYE
                     LHTTAQVAAGAGTVTLAFANNSAGNAGAVFHVYDKKHLDLVPRRYAVEAGKTLNGVWT
                     IPVADNGAYDLWVLGPNGYHREFVGNLDELEPAGGPEIQVCYEVCSPAQVVAKLYNKG
                     SRACTFSAAAKAYFTDGPWTATVQPGAVGELTWTLGGSGNWYDFVVTCDAAPSFQRRF
                     AGRVENGSDSVTDPAMGTGAH"
     gene            complement(17632..17796)
                     /locus_tag="VGC69_17410"
     CDS             complement(17632..17796)
                     /locus_tag="VGC69_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297112.1"
                     /translation="MKRKTVVRLACGLALIGALSACGDDDTPPAAPQPPAAGTPGPAA
                     CTSMHCAPAP"
     gene            complement(17910..>18209)
                     /locus_tag="VGC69_17415"
     CDS             complement(17910..>18209)
                     /locus_tag="VGC69_17415"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0297113.1"
                     /translation="STRQAIEAAFDAHGIKPQATLEIGSREMICRAVRHNLGCTLMPI
                     GDVSQDGDLRQVSIAPEAPILHVYLYCLRARAEMPMTEAFIASLPDRRKAAPTKA"
BASE COUNT         3139 a         5996 c         6024 g         3050 t
ORIGIN      
        1 ccaccgccga ctacgccccc aaccgcagcc agcgccgcgc ctggaaacag caccagcccc
       61 tgcgcgcctt cgtagccgag ctggcctggt ctcccgagca ctacgcgctc tacacccgct
      121 accagcaggg ccgccacccc ggcggcggca tggacgagga cagccgcacg caatacgcgc
      181 agttcctgct caccacgcgc gtcaacaccc gcctggtgga attccgcacg cccgaaggcg
      241 tgctgaccat ggtgtccatc atcgacgtgc tcgacgacgg cctgtcctcg gtctacacct
      301 tctacgaccc cgacatgccc ggcagcctgg gcacctatgg catcatgtgg cagattgagc
      361 agtgccgcct gctcaacctg ccctggctct acctgggcta ctggatcgcc gaaagccgca
      421 agatggccta caagtcgggc ttccagcccc tgcaaaagct gatagacggc gcctggcagc
      481 aataataaga gtgtctgagt ccgccgcggg caaggggccg tggcggcgcc tctgaggcgg
      541 ggcagccccg aaggggccga gggcgcctga tggcgcgccc gagcccgact cagcgaagcc
      601 gccacggccc cttgcccgcg gcggacgcct caagaactca aattacaatc cagccaccat
      661 gtccatccta ttcaacgcct accccctggc ccgcgccgcc ctcttcgcga tggacgccga
      721 gaccgcccat gaagtcaccc tggccaacct gcagcgcgcc tacgattgcg gcgccacccg
      781 cgccctgctg catgccaacc ccaaggctcc cgccacgctg atgggcctga aactggccaa
      841 cccggtgggc ctggccgccg gcttggacaa aaacggcgcc tacatcgacg ccctgggcaa
      901 cctgggcttc ggcttcatcg aggtcggcac ggtcaccccg cgcgcacaac ccggcaatcc
      961 caagccgcgc atgttccgcc tgccgaaagc cgatgcgctc atcaatcggc tgggcttcaa
     1021 caaccagggc ctggacgcct tcctggccaa tgtgcagcgc agccaatggc gcgagcaggg
     1081 gggcgtgctg ggcctgaaca tcggcaagaa cgccgacacg cccatcgagc gcgcggccga
     1141 cgactacctg ctgggcctgg caggcgtgta tccgcatgcc gactacatca ccgtgaacat
     1201 ctcctcgccc aacaccaaga acctgcgcgc gctgcagggc gaggatgaac tctccgccct
     1261 gctcgcccaa ctgcgcgaca agcgtgcgga actcgccgac cgacatggcc ggcaagtgcc
     1321 cctggtggtg aagatcgccc cggacctcgc gcaggaacag gtcgacgcca tcgccgacat
     1381 cctgccgcgc cacggcatcg aaggcgtcat cgccaccaac accacgctgt cgcgcacggc
     1441 cgtcgagggc ctgccgcacg cgcaggaagc aggcggcctg tccggcgcgc cggtgcatga
     1501 gctgtcgctg gccgtgatcg agcgcctgcg cgagcaggca ggccgctcgc tggccatcat
     1561 cggcgtgggc ggcattttgt cgggcaagca ggcgcgcgaa aagatcgcgg cgggcgccga
     1621 cgcggtgcag ctctacacgg ggctgatcta ccgcgggccg gcgctcgtgg ccgaatgcgc
     1681 gcgggcgctg gccgtacgct gagggcagcg tacggctgcc tggacgcccg cttgtgatgc
     1741 taatggccca tggggcttat cccccattgg ccacagcctg ctcgagcacc aggcgcacgt
     1801 accgcgtata gggaacaccc gccgccttgg ctttggcctt cacggcatcg agcaaggact
     1861 gtggcaaacg catattgagc gcggccgatt taggctcgac ctcgaaacgc atgggcttga
     1921 aaccggacag gtcgtactcg gacagatcgg ccgtttcgac gaaatgctcc gcctgcgtat
     1981 cgctcttgag cgagggcatg ggcttgaact tagatttgct cataacgcgc gatctccttg
     2041 caatgcatat accgtgcgct aataggccgt atggttgccc tcgaacacct cttcgatttc
     2101 ctgcttggat agcccatgct ttgcgcattt aggccagttc ccggcatccc aatcgaagcc
     2161 gtctattttg aacgccataa aattctacta cacttttgtc tatacaattg tgtattcaat
     2221 tgcatttcaa ccgaccgctg cccagccagc atatccaccc ggcatgaatt cgacgacgcc
     2281 tggcaggaca aaagcctctc tttggcgtat ggccagacaa taaaaaaggg ccacccgaag
     2341 gtggcccaat tccatctctg ctcagttgct cagaggtact acttgcagca gtatcgctac
     2401 tgctgtgcta cgacaaccgc aggccccagc gggccctaag cctttaggcg gcgcggcggc
     2461 gggtcgtagc tttgccggcg gcttcggcaa catcggtggc ggccttgaac gtggcattgg
     2521 ccgcggcgtt caagttggtt tccgcgactt cggcggcctg cttggcggcc ttggtcagcg
     2581 attcgtaggc gctgttggcg gtagccagcg acgacttcag catggcgacg gcgctttccg
     2641 aaccggcggg cgcgttcttg gtgatctgct cgacggcggc attcacttgc tcgcggcctt
     2701 cggcgatctg ggcttcggcc agcttggcca gttcggcctg cacgccggcg aagatgtcgt
     2761 acacgtgctt gccgtaggcc atggccttgt cggcgcccgg ctggagggcg gcggacacga
     2821 aggaagatgc gtcctgggcg tccttcagct cggcggcttg atgggacttc tgggcggcct
     2881 cgtccagcgt ggccttgacc accttgaggt tcaggtcgac cagcttctcg aagccttcga
     2941 acacggcggt ctgggcggcg atgagggtgc cgacggcggc tttctggcgg gccagaactt
     3001 gttgcggaat ggcgctcatg gatatctcct tcctgagatg agtttgggcg gcctggctgc
     3061 taccgcctgc gaattggggt gttaccagta ggggcatgac ctgcagggtg gggcaagcgt
     3121 tttttgttgc gctgcacaaa gcctattcta gtcttatgaa aaagactgtc aagtattttt
     3181 tgtgcaacgc agcatatatt tcgggattca ttgcccattc gaagatttcc ctgtccggtc
     3241 agggtgctgc aacgcagtat gcccgccggc cgcacccgcc ttcatggggg aaatacgggg
     3301 taaccatccg ccgccatttt ccctagactt gcgtcacaca ggcggcgagc cggcaggcac
     3361 cgccgcctga catgggaata cacaagcatc acacagagca tcccagccat caacggagac
     3421 aaataatgaa tcgcatgacc cgcaccctgg cggccacgct gggcgccggc gccctggcgc
     3481 tatccgccac cgccgcccag gccgctgact tccccgacca caccatcgac ttcgtcgtcc
     3541 ccttcgcggc cggcggcccc accgacaccg tggcccgggc cctggcccag gccatgagcg
     3601 cctcgctcaa cggccagggc atcgtcgtcg aaaacaaggg cggcgccggc ggcaccatcg
     3661 gcaccagttt cgtggcgcgc gcccagcccg acggctacac cctgctgctg atgcacgtgg
     3721 gcttctccac cgccccgggc ctgtacaaga accccggcta cgacccctac accagcttcg
     3781 agcccatcgg cctggtggtg gacgtgccca tgaccgtgct ggcccgctcc gacttcccgc
     3841 ccaacaccct ccaggagctg gccacctacg tcaagcagaa cgccagcaag atcaccctgg
     3901 ccaacgccgg catcggcgcg gccagccatc tgtgcggcac catgctcacc agcgccctgg
     3961 gcgtgaacct gctgaccgtg ccctacaagg ggacggcgcc ggccatgaac gacctgctgg
     4021 gcaagcaggt ggacatgctg tgcgaccaga ccaccaacac cacgcagcag atcaacgcca
     4081 agacggtcaa ggcctacgcg gtcaccagcc tcacgcgcgt gcccaccctg cccgacctgc
     4141 ccaccatgga cgagtccggc tacaagggct tccaggtcgg catctggcac ggcctgtggg
     4201 cgcccaaggg cacgcccaag cccatcgtcg acaagctggt caaggcgctg caggccggcc
     4261 tggccgaccc caagttccag gccaatatga agcagctggg caccaccgtg ctcacctcgg
     4321 aggccaaccc ggccgcgctc gacgccaagg tcaagcagca ggtgccccag tggaccgagc
     4381 tgttcaagaa agtgggcgtg gaaaagcaat aagcggatcg gaatccgcct gatgcgaagc
     4441 ccggccttgc gccgggcttc gccgtttcag tgcgacagga agctgcgcag ctcgggcgtg
     4501 cggggcgccg aaaaaatgtc ttcaggcgcc ccttgctcat ggatgcgccc ctggtgcatg
     4561 aacaccaccc ggtcgctgac cttgcgggca aagcgcattt catgcgtcac gatgatcagc
     4621 gtggatccat ccttggcgag tcgctcgatg acttgcagca cctcgccgac cagctcgggg
     4681 tcgagcgccg aggtcatctc gtcgcatagc agcacctccg gatccatggc cagggcgcga
     4741 gcatgatgtt ttcgcccgcg gtcatggaag ggaaaagatt gaagtgctgg aagatcatcc
     4801 cgaccttctg cctcagctcg cgcagcgcgg cggcatccgc cgtttccacg cgctggccgc
     4861 cgaccacgat ctcgccttga tcgaaagcct ccaagccgtt gatgcagcgt agcaaggtgc
     4921 tcttgcccga accgcttcta ccgatgatgc agaccacttc gccgcgggca acctgcatgt
     4981 cgacgccttt caacacgtcg ttttgaccga agcgcttgtg caagcggcgg atatcgacca
     5041 gcggcgtggg ggtttcagct ttttgcatgg ttcattctca atgccggaca cgcaggcgcc
     5101 gctccatcca ggctccgaag agcgaacatg gaaagcacag cgcaaaatag aaaaaggcaa
     5161 ccagtccata gaccagaaat ggctggaagg tcacgttggc gatcatctgg ccggagcgcg
     5221 tcaattccac gaacccgacc acggaagcca gggacgtgtt cttgatgatc tgcaccatca
     5281 ggcctatggt cggcgggatg gacagccgga tcgcctgcgg gaagatcaca tggcgaagct
     5341 gctccgtaaa cgtcaaggcg aggctggcgg aggcttccca ttgccctcgg gggaccgact
     5401 ccacgcagcc gcgccaggtt tccgtgaagt aggcgctggc atagagcacc acgcatatcg
     5461 aggccgccgt catcggcgtg gtttccacgc cgaccagggc cagcccgaaa tagaccagga
     5521 acagctgcat gagcagcggc gtgccctgaa atacctgcac atacgcggca atgggcaggc
     5581 cgaatgccct gggccgcgcc aggcgcagga ccagcagcaa gacgcccacc acggccccgc
     5641 ccaggaatgc ctcggccgac agcagcagcg tccaggggat ggagagcaac aggttgcgaa
     5701 agacatccca gaaggtgaac gggatcatcg gccggctccc acgggcgaac agccgaagat
     5761 gaagcggggg ccgagccagt gcaagaaccg gcgaaagcag atggacagca gcaaatagag
     5821 gatcgtcacg atgatgtagg cctcgaactg ccggaatgtt tcggcggcga tgcgattcgt
     5881 gacataggag agctcttcgg tcgatataag gctgcaaacg gccgtcgcaa gcatgatgat
     5941 gacgagctgg ctcgtcatgc cgggccagac gcgcgacatg gccggcggca gcacgacgcg
     6001 cgtgaacgac tgccaccggc ccaggcccag cgattcggcc gcctcgatct ggccgcgggg
     6061 cgtggcctcg atgccggagc ggatgatttc gcagccatag gcggccaggt tgacggtcag
     6121 cgcgagtatc gcggcgggaa agctggcgaa cttcaaaccg atggccggca ggccgaaata
     6181 aatgaagaaa atctggatga tgaacggcgt attgcgaaag aactccacat agcagcccac
     6241 cgcgagcctc agccggcgcg cgccgtatac ccggcaccag gcgcaggcga tgccaagcag
     6301 cgtcccgcag cagcaggaaa aaaacgtcag caagaccgtc atggccaacc cgcccagaaa
     6361 ggcgggccag gcggccaata cgctggagaa attgagcatc gatgcttccc cttatcgagc
     6421 cgggctatcc acgcatgcca tcgggatcac gccaggccgt cgaacaatgt ttgtatggcc
     6481 agcgccgaat ccagcccatg cagcgtttcc agcaggcgtt cggcacgccc ggcgccgacg
     6541 gccggcatgg cgcaatccag gaatttgcgc gctatccgtg attcgcccag cggttcccgc
     6601 gggttgccca cggctttgtc cttgcttgcc tgcagcacgc gcccatcgcg caacgccacc
     6661 cgcacttgcg ccccctccgg cgcgcgccgg cgtacatccg cgtcattcca atgcggaccg
     6721 gtcaccatgt cgacccgccg catcagcccg cgcaggccat cttgcgcaag ccgatgcggc
     6781 gccatcgcgg ccaagcccca gtccgggcgt tgcaaggtcg ccgccatggc gaacggcatg
     6841 ctaaagcgcg cctgctccgg cgtatccggc acgccatagc gcaaattggc gacgacgatg
     6901 tccggcacgt cgcagacgat gcggacgacg tcatcggccg aaaaccggta acgcgccatc
     6961 aattcgccca aggcatcggc ggccgcatgc gatgacaggc agaccggggg aatagccgct
     7021 ccccaggccc aggatcgtgc acgggccctg ctcgtgcggc aggcataacg cctgcgccgc
     7081 accggatccg gcacctggtc atccgacaat tggcacgccc agcgcgccag gcgttcgctg
     7141 gcgatcgatg catggctgtc cacgcgtctc ccctgtgttt gcctggccgg cgcgcgtggc
     7201 cgcgcccggc ccatcaaccg ttcgcgcgaa cggccttatt cgggcagatc acccgccggc
     7261 gcgcccatcc aatgcagcga cattcgatcc agcgtcccgt ttttcttgga ctcgcgaatg
     7321 atgtcgttga ccttggccag gagcttgggc tgatccttgc gcacgccgat atagcagggc
     7381 gaattggcca gcacgacctt cagctccatg tccagcttgg gattgcgctg cgtgagcgcc
     7441 gcggcgacgg gcgtgccgct cgcgaaaaac tcggtctgcc cggaaaggaa agccgaaatc
     7501 gtgctgctgt tgtcttcgta gcgcttgacg actgctttcg gcgccaggtc cttgagctcc
     7561 tggtcctgca tcgaaccgcg cgtcacggcg atctctttgc tggacagttc gtcatacgtt
     7621 ttcgccgtgc tgcccttggc gccgaagatc gcgtcgaaga aaggcgcgta ggcgatgctg
     7681 aaatcgatga cctgggcgcg ctccgccgtt ttgccgagcg acgaaatcgt cagatcggtc
     7741 ttgcccgttt gcagataagg cacccggttc ggcgccgtga caggcaccag ctcgactttc
     7801 acgcccagct tttggccgat gaggttcgcc atttcgacgt ccaggccttg cgccttcatg
     7861 tccggaccca cgaagccgaa aggcgggtag tcggtcggca cggcgacgcg gagggtgccg
     7921 cggctggtaa tttcatccag gaggtcggcg gcctgcgctg gcaaagccgc gatgcacgac
     7981 aagacacttg cagccccaat gatccatttc ttgattttca tgttaagtcc tcgataagtg
     8041 cagccgttca tagttgaaaa gcaccagagg ccgcctgaat caggacggcc cggtgctcta
     8101 tgcgacgcct cagcttcccc ttgagggtcg atccgggggc tgtcgcccct cggtttatca
     8161 gtcgggaatg acggcggtga cgtcgatctc gaccagatat tcggtccggg caaaagcgct
     8221 gatcaccagg ccggtggaaa cgggaaagac ccccttcagc catttgccca tcacgttgta
     8281 gacgtcttcc cgatagcgcg gatcggagat gtagatgacg accttgcaaa tgtggtccag
     8341 cttgctgccc gcttccttga gcagcatgtc gatgttcttc atggcctgtt cggcctgccc
     8401 ggcgacgtcg ccgatcgcga cgcacgccga ggtgtcgaga ttctggccga tctggccgcg
     8461 caggaagacc atggtgccgc gcgcgacgac ggcctggcag agatcgttgt cgatcctctg
     8521 ttccggatag gtttcccgag tgttgaattt gcgaatgcga gtgtgtgcca tggtgcgaag
     8581 cgtatccagg ttgtcgagaa gcggacgaat caggcgcggt cgaaataacc cgcgcgtatg
     8641 ctgctgccga tgaggttgac gatcagccgg cccgcggtca cgcaggtcag cttggtcgcc
     8701 gtatccttgg acggctggat ttcgacgatg tccatgccga cgacccggcc cttggccacc
     8761 agcccgtgga tgagcttgcg ggcctggacg aaagtcacgc cgcccggcgc cggaccgtcg
     8821 accgcgggca tgaccgtggc gtccatgccg tcggcgtcga tcgagaggta gtagttgccg
     8881 ccatcgggga tgcggtcaag cacggcctgc atgcctacgt catgcagttc ataggcggtg
     8941 atcagttccg cgccgtatgc cttggccgcc tcgtagtccg cgggccggcc gctgccttgc
     9001 gcgcgcaggc cgatctggat gatcttgccc acatggggca gttccgaggc gcgccggatg
     9061 gggctggaga ggccgtcgcg aatgccgttg acctcatcgc gccaatcgag gtgggcatcg
     9121 acgtgcacca gggtgatctc ctcgtgcagt gcttcgtagg ccctgagcac ggggttggtg
     9181 atgccatgat cgccgcccag gacgataggc atgccgccgc cgcgcagaat gtggcggatg
     9241 gcctcttcgc aacggcgcag gtgcccgccc gggatgccca ggtcgggaat gacgtcgccg
     9301 cagtccacga aacggatatc cgcgcggcct tgcagcagcg gaccgtcgat gtcgaagtca
     9361 tagtgttccg gcgcgcgcac catgcggtcc gtgacatcgc ggattgcctg cggcgcgcga
     9421 gtctggtcat tgctgaaggc ttgcggcgcg tagggcgagc cgaacggcat gccgaggacg
     9481 gcgatatccg agttcaagcc ttccatctcc gtgaccagtt cggaatagag cagcgtttta
     9541 tgtccggttt tggggggaac ggtaagcggt gggttggcca tggttttgtc gcgattaagg
     9601 ccgatgggaa cggccaggaa gaaggtgcgc gagaaacttt atccatggcc ttgaaattag
     9661 aaaaatgaat tttttatcga tatagtcttg taattcaaga cctttatctg ggtattccct
     9721 aggtgccgct gccatgctca agctggattt cgacgagcgc gacctgcgct cgctgcgcgt
     9781 tttctgccac gtcgccgagg cgggcggatt cgccgcggcc gagcagcgcc tgctgatgtc
     9841 caaggcgtcg atcagccgcc atgtccggga tgtggagacc cgcctgggcg tgcgattgtg
     9901 cgagcgcggc ccaggcggct ttcgcctgac cgcggaaggc gccgtggccc tgaacctggc
     9961 gacgaccgca ttgcgggcgc taagccgcat ccggccggaa atcgatgcgg cgcatggcgt
    10021 cctgtccggc cccttgacca tcggcctcgg cgaacacacc ttgacgcatc cgacctgccg
    10081 gctgcccgag gccctgagcg cgctgcagca gcaagcgccc catgtgcagc cggaaatcgt
    10141 ggtcatgtcc ttcaaggacc tggatcaggc cctgcgcacc cagcgcatcg acatcgccat
    10201 acgcggcaaa tacagcgagg accgcgactt cagctatttg ccgctttata tcgagacgca
    10261 tcgcatctac gtgccgagcc gggtaacccg cgccaaagac caggcccgcc tgccgctggt
    10321 gtaccggccc cacccctatg tggatcgggc gctggccacg ggacgctacc agcgcggccc
    10381 ggaagcaagc ggcctggacg gcgtcggcgc gctcgtcgcc acgggctatt accagggctt
    10441 gctgcccacg cactatggcg aactgctgca agagcgcttc ggcttgcgcc aggccgggcg
    10501 cggtgagacc tactcgcacg ccggctgcgc ggtcatgctg gcatcgcggc agctgagcca
    10561 gcgggccgag ctgttcctgc gtattctgcg cgccctgcac cccatcaaag gcaaggccct
    10621 gccggcgtcg gttcgcgcct aaggcgcggc tgcgcaagcc cggcaggcgg cagccggccg
    10681 cgcctcagcg cgccgccgac acgcccgcct cgtagcccag cgcctcggaa atccccgcgg
    10741 cggtatggat gagcaggtct atccattcgt cgcgcatgcg ctcggcgggc gccgagatgg
    10801 acaagcccgc caccagcttg ccggtgtcgt cctggatgcc ggcggcgatg cagcgcacgc
    10861 cgagctccag ctcttcgttg tcgcgcgcat agccgtggcg gcgcacctcg gtcagctcgc
    10921 gctgcaggcg gtcgatgtcg gtgaggctgt tgcgcgtgtg cccggacagc ccggtgcgct
    10981 gcgcgtaggc gcgcacctgc ctggcgtcgg cgatggacag gaacagcttg cccgtggagg
    11041 tcaggtgcag cggcgcccgg ccgccgatgg cgcgcaccac ctgcatgccc gagcgctcgc
    11101 tccaggcgcg gtcgatgtag acgatctcgt cgccctgctg caccgacagg ttgatcgtct
    11161 gccccgtctg ccggtgcagg gtgcgcatgg ccgattgggc ggcctcgcgc acgttcagcc
    11221 gccccttgac cagcgagccc agctccagca ggcgcatgcc cagctggtac aggccgttgt
    11281 cgacccgttc cacatagcgc cccaccacca gatcgttgag gatgcggtgg gccgtggagg
    11341 catgcaggcc ggtggtggcc gacagctcct tgagcgtgac ggggtcgggt tgggcagcca
    11401 gggcgtcgag caggcgcatg gcccggtcga tgacctggat ggcaacctcg gacgagcgga
    11461 tgccgctctt ggagtcggca tccggctgcg cagccggggt gggagagtcg ggggatgagg
    11521 cgggcatatc ccgtattgtg aaatcttgca cgggttttgg caatccggcc cgtcccgccg
    11581 ggccggattg ccagcgggcc gcctacgggg tcagccgccc gcccaggcgc tcgacttcgg
    11641 cgtgcaggaa ggccagcgcc tcggccgcgg ggccgggctc gcccttgacg cccagctcga
    11701 tgtgcggccg gttgccgtct tcgcccacgc tgggcaggct gaaggcgcgc acgccaggcc
    11761 agcgctgctc cacggtttcc atggccggcg tgatgcgcga ctcgggcagg ccgtagatca
    11821 ggaaggcgtg ctcggcgcgc tgttcgacgt ggtgcagcgc gcggtagcgc gtgtcgagcg
    11881 tccattcgag catgggccag gccatcaccg gaaaacccgg cacgaaggta tggtcctgga
    11941 tgaagaagcc cgggatgcgg ttgtaggggt tgggcacgat ctcgcagccc cgcgggaaca
    12001 cgcccatctg caggcgctgc tggtgttcgg gcgtgctcat gtcgggcgcg ggacggcctt
    12061 ctttctgggc catctcgatg gtgcgctggg tgatggcctc gcgcgcctcg ggatgcagtt
    12121 ccagcggcag ccccagcgcc gcggccgcgg cctggcgcgt gtggtcgtcc ggcgtgccgc
    12181 cgatgccgcc gcacgagaac acgatgtcgc ccgaggcgaa gctgcgctgg taggccgcga
    12241 ccagctggtc gcgctcgtcg cccaggatct cggcccaggc cagctgcagg ccgcgcgcgc
    12301 ccagcatttc gaccactttg gagaagtgct tgtcctgccg gcggccggac aggatttcgt
    12361 caccgacgat gatgaggccg atgcggcgag aggaagtagc catgcgtggt tccaaaaaga
    12421 ttcagacgtc gatcgtacgc tcgccgcgca ggcgcgcaag cgcatccagg ccgtaatggg
    12481 catacaccag gctgctgtat accggtagca ccacccaggc gggcggcagc aggttgatga
    12541 gggagcagac gagcccgatg ccccagaaag acatattgtg ccgcttgagc agcaggcgcc
    12601 gctcggccgg gctggcgtga tcggcaatgg catccacgcg cagcatgcgc gagaaggcga
    12661 aggcccacca gaacacgtgc agcagcaccg ccatgggcgg caccagccac aggggcagcg
    12721 tcagcagcca gcccacggcg aacaccgcgc tcacccagag ggcgttccag acgctgaccg
    12781 ccgcgacgtg gcggccttgc cgcgccagcc ccgcgtactc gccgcggccc aggtggccca
    12841 gcaccagcgg catgacgaac accgccgcga tcgccaggcc caggatgccc gacaccggca
    12901 gcagaatggc cgccgcgatc accggggtga gatagagctt gagcgaaaac aggcccaccg
    12961 tcgtcagcca gccgtcgatg cggtcgatgg cgccccagcg ggcgaactcc atgcgcagcc
    13021 agtcggtcag cggcatccag aacagccaca gcagcaggat ggccccgacc agcgcgatca
    13081 ggaagggcag cagcaccgcg aacagcatgg ccggatggca ctgcgacacc agcgcgcgct
    13141 tgaacgcccg cgccacggcg gcggactggc cataaggccg ggagacattg cgggagggat
    13201 agctcatgga cggcgggcgc caaccggaag aggaccaaat ggcgggtatc ataggtcaaa
    13261 gaccccgcga tttgctcaca tgtctctttt cgtacccgga accgacgacg cggccctgcg
    13321 ccaggccctg gccgcccccg gcacctggct ggtggcctgc ttctgcgccg cctggtgcaa
    13381 cacctgcacc gaataccgcc ccaagctgca agccctgtcc gaggccaggc cgcaacacgc
    13441 ctacgcctgg atagacatcg aggacgaacc cgatctgctg ggcgacgaag acgtcgagaa
    13501 ctttcccacc ctgctcatcc aggcgggcgg gcgcgtgctg ttctacggcc cgatgctgcc
    13561 gcatatcggg catctggagc gcctgctcga caatctcggg atggacagcc ccgccgtcag
    13621 cacgcagttg cccgacgtgg cgcaacggct ggcgcagcgg taattacgag gccttgcggc
    13681 cgccgccgag caaggccgcc aggggccgct tgcccgccgt ggacgcgggc ttggatgcct
    13741 ccgactcggc cgacgggtcc tgcgagggtt cgtagggctt gaggaaaaac tcgtcgactg
    13801 gcgcggcgcg cggcgcgcgg tggtcgtggg gggagcgctc gcggcgctcg ccgcgctcga
    13861 cacgggggct ccgttcgcca cgatcgcccc gttcacgttc gccggaagcg cggctcgacg
    13921 cctcggccgg caggtccagc ttgccgcgcg gcacctggcg cttgatcagc ttttcgatgt
    13981 cgagcaagta gcgctcttcg tcgggcgagt acagcgcgat ggcctcgccc gaggcgccgg
    14041 cgcggccggt gcggccgata cggtgcacgt agtcttcggc gctatgcggc aggtcgtaat
    14101 tgatgacgca gggcacaccg gccacgtcca ggccgcgggc agccacatcg gtggccacca
    14161 gcacttcgag ctcacccgcc ttgaaggcct cgagcgcctt catgcgctcg gcctggctct
    14221 tgtcgccatg gatggactcg gccttgacgc cgtcgcgctc gagcaggcgc gccaggcgcg
    14281 ccgtgccgat cttggtgttc gagaacacga tcacctgctt gagcccgcgc gacttcacca
    14341 gatgcaccac cgccgcgcgc ttggcctcgc ccggcatggc ataggcgacc tgcgtgacgg
    14401 tgtcggccgt ggcgttgcgc gccgcgacct cgatctcgac cgggtcgttc aggaaggtgc
    14461 ggcccagctt gcggatctcg ttgctgaagg tggccgagaa cagcaggccc tggcgctgcg
    14521 ccggcagcag ccggatgatg cggtcgaggt cgggcagaaa gcccatgtcg agcatgcggt
    14581 cggcctcgtc cagcaccagg atgcccacct ggcccaggtt gacgttcttt tgctcgacgt
    14641 ggtcgagcag gcggcccggc gtggcgacta gcacttcgca gccggcgcgc agcgcctctt
    14701 tctgcgggcc gatgtccacc ccgccgaaca ccacggccga gcggatcggc gtgtgcttgg
    14761 tgtagcgctt gacgttttcg tagacctggt cggccagttc gcgcgtaggc gtcaggatga
    14821 gcgcgcgcac cgggtggcgg gccggcgagg cgctggtatt ggccatcggc atcaggcggt
    14881 gcaggatggg cagcgtgaag gccgccgtct tgcccgtgcc ggtctgcgcc gcgcccatga
    14941 cgtcgcgccc ctgcattgcc acgggcacgg cctgcgcctg gatgggcgtg ggcgtggtgt
    15001 agccggtatc ggcgatggac ttcagcagca gcgggtgcag gtcgaattcc gcaaacgtgc
    15061 gggaagcggc ctcggcggcg ggtacgtccg cggcgggcgt gtcgatagga ataggttgac
    15121 tcatcggttg ggctccggac ggcgcgaaaa aagaagcgcc ccggatggct gtagataaac
    15181 gattttagca gcctgcgcct gacaggccgc ccaagcgccg gcaaacggcc ttgcaaggca
    15241 aaaaaccgcg gcctcttccg aggccgcggg tttttcggcc gtgatgccgg gtgtgcctgt
    15301 tttttaatgg gcgcctgtgc ccatcgcggg gtcggtcacc gagtcgctgc cgttctcgac
    15361 ccggccggca aagcggcgct ggaacgaggg cgcggcatcg caggtcacga cgaagtcgta
    15421 ccagttgccg ctgccgccga gcgtccaggt cagctcgccc accgcgcccg gctggacggt
    15481 cgcggtccac ggaccatcgg tgaaataggc cttggcggct gcgctaaagg tgcaggccct
    15541 gctgcccttg ttgtagagct tggccaccac ctgcgcgggg ctgcacacct cgtagcagac
    15601 ctggatctcg ggaccgcccg cgggctccag ctcatcgagg ttgccgacaa actcgcggtg
    15661 atagccattg ggtcccagca cccatagatc ataggcgccg ttgtccgcga ccggaatggt
    15721 ccacacgccg ttgagcgtct tgccggcctc gaccgcgtag cggcgcggga ccaggtccaa
    15781 gtgcttctta tcgtacacat ggaacacggc gccggcattg ccggcactgt tgttggcgaa
    15841 cgccagcgtc acggtgcccg cgccagcggc gacctgggcc gtggtgtgca gttcgtaggg
    15901 cagcgcgcgc gagggcagcg tgcccgcggc ctgaaccggc aggggggagc cgggggtggc
    15961 ggtggacttc ggcaccgcgc ccagggcggc ctgcgacgcc gccaggttgt cggccgtcac
    16021 cttgtccgtg cggccggtca gggtggccag cggcgcgctg ttcggattga cgaaattgaa
    16081 gcacgaggtc aggtcgccgc agatggcgcg gcgatagttg ctgatctgcg gctccttcac
    16141 gccgaagcgc ttttccagga acatcagcgt cgaggtgtgg tcgaaaacct gcgaatcgac
    16201 gaagccgccg cggctccacg gcgagaccac ccacatcggc acgcgcgggc cggggccgaa
    16261 gggccggccg tcgaccgccg gctgagtgcc gtcgatcggc gcatagttgt ggtattccac
    16321 ggccatgtcg ctgtcggaca aggtctggcc gcctgccagg gtgccgtcgg gattgtgcgc
    16381 gggcgcggtc ggcggcggca ggtggtcgaa gaaaccgtcg ttctcgtcga aattgatcag
    16441 caggacggtc ttgctccaca cctcggggtt ggcggtcagc gcatccagca cctcctgcac
    16501 gtaccagccg ccctgcgccg ggctcgaagg cccggggtgc tcgctgtaga tcgaaggcgg
    16561 gatgatccac gacaccgcgg gcagattgcc gttctggatg tcgtcgcgga aggtttcgag
    16621 aaaaccgtag ggcatggtgt tgccgaagcc cttggccagc gggctgagcg cgtcgtcgat
    16681 ggccggatcg tagaacgggc cggcggcggt ggcgggctgc gtctggatgt tttccgtggc
    16741 cacgtagatc gggcggcgcc cggccggcat gtcctcgatg gccttgcgcc aatgacgaaa
    16801 gcccatcatc tcgttgcaac cgtagttgtc gatcaggctc tggtagacct tccacttcac
    16861 gccggccttt tccaggcggt cggcataggt ggtccaggtc cagccggtgg tggacgggcc
    16921 gatgtcgttg ccgccgttga actcgttgtt caggcccgcg gtcggggtat aggcgccgcc
    16981 attgggatcg gtgccgttcg ggccgttgga gccggtccag tagaacaggc ggttggcgat
    17041 cgtgcccgtg tgcatcgagc agtgatagtg gtcgcacagc gtgaaggcct cggccagcga
    17101 gcgctggaac ggcacttccg ccgcctcgta gtagcccatc gacagcggcg tcttgctgtc
    17161 gggccagttg ctcatgcgac cgctgtccca ggcggcctgc gcgtcgaccc aggtatgcgg
    17221 cgtgccgccc gcgcggatcg cgttgccggc ggtctcgtcc aggtgatagg gaatcagcgt
    17281 gccggcgccg ggcgcggccg cgcccgtgta ggtctggtag aagatattgg gcgtgcccgg
    17341 agccggaacc ggaaagcggt cgccgtagcc gcgcgcgccc ttgtaggtgc cgaaatagct
    17401 gtcgaaggaa cggttctcca gcatcagcag caccacatgc tgcacgtcgt tgatggtgcc
    17461 cgtggcgttg ttggcctcga tggcgagcgc gcggcggata gccggcggga aagtgctcag
    17521 cacggtcgcg gcggcgccca tgccgagcga gctgtgcagg aatctgcgct tggaagcatt
    17581 gaaggtcata ccgttttatc cctgaattga cgtgaacaag ccgggccgct ctcagggcgc
    17641 cggcgcgcaa tgcatggacg tgcaggcggc cgggcccggc gtgccggccg cgggcggctg
    17701 gggcgccgcc ggcggcgtgt cgtcgtctcc gcatgcgctc aacgcgccga tcaaggccag
    17761 cccgcaggcc agccgcacta ccgtctttcg tttcatttcc acccctggtc accgttgcgg
    17821 ttcgacggca tatccggcga accgaagaca ttctggcggg gtgcggcgac gccggcgtgt
    17881 cattccggcg aaacctgcct attgcggcat caggcttttg tgggtgcggc cttgcggcgg
    17941 tccggcaggc tggcgatgaa agcctcggtc atcggcattt cggcgcgcgc gcgcaggcaa
    18001 tacaggtaca cgtgcagaat gggcgcctcg ggcgcgatgg acacctgccg caggtcgccg
    18061 tcctgggaca cgtcgccgat gggcatgagc gtgcagccca ggttatggcg cacggccctg
    18121 cagatcattt cccggctgcc gatttccagg gtcgcctggg gcttgatgcc gtgggcatcg
    18181 aacgcggcct cgatggcctg ccgggtcga
//
LOCUS       DASWPO010000328         6385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2847204, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000328 DASWPO010000000
VERSION     DASWPO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847204"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..946
                     /gene="pssA"
                     /locus_tag="VGC69_17420"
     CDS             170..946
                     /gene="pssA"
                     /locus_tag="VGC69_17420"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY0297114.1"
                     /translation="MTNMPQRDAEHRHRSIYLLPNAFTTAALFAGFYAVVQAMNARFD
                     IAAIAIFAAMVLDGMDGRVARLTNTQSAFGEQYDSLSDMTSFGVAPALVAYEWVLQDL
                     GRWGWLAAFVYVAGAALRLARFNTNLAVVDKRFFQGLPSPAAAALVAGFVWLSVDNKL
                     PIHDHLMAWTTFGLTMYAGITMVCDAPFFSGKKFALGRSVPFWGILLVVLVFVFVSSD
                     PPVILFVLFVLYGLSGWVTWAWRWSRARRLQQERRGGAGQ"
     gene            complement(940..1455)
                     /locus_tag="VGC69_17425"
     CDS             complement(940..1455)
                     /locus_tag="VGC69_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297115.1"
                     /translation="MRHLLFRCGAAAALLAGLSACQNIDRAVKPGDAYAAVLARYGSP
                     KLVCPRPDGGQRAVWTLQPLGQYAWGVNVGADSRVERAEAVLTDEHFKVLDTGDWTPD
                     QVRCEFGPPSEVETVGLGDMREVVWSYRYKEQGVWNAQMHIFMGPKGDHVTHHFHGPD
                     PIYERSRLGFY"
     gene            1652..1921
                     /gene="rpsO"
                     /locus_tag="VGC69_17430"
     CDS             1652..1921
                     /gene="rpsO"
                     /locus_tag="VGC69_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0297116.1"
                     /translation="MSVADINKADIVSQFQRAQGDTGSPEVQVALLTARINELTGHFK
                     EHMKDHHSRRGLLRMVSRRRKLLDYLKGRNPDAYRALIEKLGLRK"
     gene            2027..4168
                     /gene="pnp"
                     /locus_tag="VGC69_17435"
     CDS             2027..4168
                     /gene="pnp"
                     /locus_tag="VGC69_17435"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0297117.1"
                     /translation="MFNKVTKTFQYGQHTVTLETGEIARQASGAVVVSIEDTVVLATV
                     VAAKKAKPGQDFFPLTVDYIEKTYAAGRIPGGFFKREGKPSEKETLTSRLIDRPLRPL
                     FPEGFYNDVQVIIHTLSVNPEIDPDIAAMIGSSAALAISGIPFNGPIGAVRVGYADGQ
                     YVLNPTASQLKDSQLNLVVAGTETAVLMVESEAKQLSEEVMLGAVVYGHEQMQTVINA
                     IHDLVRDAGKPEWDWQPAAKNEALIAAVAAAGQEGLVAAYQVREKQARTTKLREVSAS
                     VKAKLAEQAAAAGQDVPYGVTVDNILFDMEARIVRGQILNGEPRIDGRDTRTVRPISI
                     RLGVLPRAHGSALFTRGETQALVVATLGTKQDEQIIDALMGEYRDRFMLHYNMPPFAT
                     GETGRVGVPKRREIGHGRLAKRALAALLPAHEDFQYTIRLVSEITESNGSSSMASVCG
                     GSLAMMDAGVPVKDHVAGVAMGLILDEGKFAVLTDILGDEDHLGDMDFKVAGTVNGVT
                     ALQMDIKIQGITKEIMQVALAQAREGRLHILDKMREAQDGSRGELSAFAPRMISMKIN
                     PEKIRDVIGKGGATIRALTEETGTQIDIQDDGTIVIASADENKAKEAQRRIAELTAEV
                     EVGQVYEGSVLRLLDFGAIVQVIPGKDGLLHISEIANYRIANINDVLKVGQQVRVKVI
                     EADDKGRLRLSIKALGGIEQQQQPETAPQAE"
     gene            4449..5174
                     /locus_tag="VGC69_17440"
     CDS             4449..5174
                     /locus_tag="VGC69_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297118.1"
                     /translation="MATGRWLLLGVWGILLLAGCSSTPPGKDGGTPNGESMSVDQMVQ
                     TDFNRTVTMAMRDNLNGLYVLLDKLYRRNPREWRKSGQADEKAAVQRVKTLIEQRQPP
                     AGLAGLRDVQILAVALNPNYSDDRVAAFVYGLADTIIAAHGDKVRFHALDVLDGQRIY
                     NAARNVEAADWLLSTRRAPGGGPLLLSNEMGDGKVNLSFEREFGALIGRLDLEANLLG
                     ENIRRIGINYAQSLMFFNFLPVR"
     gene            5189..>6385
                     /locus_tag="VGC69_17445"
     CDS             5189..>6385
                     /locus_tag="VGC69_17445"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY0297119.1"
                     /translation="MIDLSTIQAARQRLQGQVLKTPFMLSRTLSDMLGAKIWLKFENL
                     QFTASFKERGALNRMLALSPQERAKGVIAVSAGNHAQGVAYHAQRMGVPAVIVMPRFT
                     PTVKVANTRRFGAEVVLAGDTFDDAQAHGYELVGQRGLVMIHPYDDEAIIAGQGTLAL
                     EMLEDQPDLDLLVIAIGGGGLISGVATAAKGIKPGIQVIGVQTERFPAMYAAVHGRDM
                     QPGQYTIAEGIAVKSPGRITRKIVGDLVDSIELVSEADIEHAIVVLLEIEKTVVEGAG
                     AAGLAALLSARARGQTGWFQGKRVGLVLTGGNIDPLMLGELIERGMVRAGRLARVRVS
                     ARDVPGSLAHITATVAEAGANIDEVHHQRAFTMLAAQNVDVELVLQTRGRTHLASVLD
                     ALHAAGF"
BASE COUNT         1162 a         2126 c         2025 g         1072 t
ORIGIN      
        1 agaaatccga agcgcaggcg ccagtggcgc cgagcatcgg aatttcgcag ggcagcctcg
       61 gcggcttaaa gaaacaatcc ccggctgctt gagaacgacc ggaccggctc aagaacacac
      121 ccaacttcgg ttaaccttca ccctgttcgt ggtttacaag gttaccgata tgaccaatat
      181 gcctcagcgc gatgctgagc atcgccatcg cagcatctac ctgctgccca atgccttcac
      241 cacggccgcc ctgtttgccg gtttctacgc ggtggtgcag gccatgaacg cccgcttcga
      301 catcgccgcc atcgccatct tcgcggccat ggtgctcgac ggcatggacg gccgggtggc
      361 ccggctcacc aatacccagt cggccttcgg cgaacagtac gactccctct ccgacatgac
      421 ttccttcggg gtggcccccg cgctggtggc ttatgaatgg gtcctgcagg acctcggacg
      481 ctggggctgg ctggcggcct tcgtctatgt ggcgggcgcc gcgctgcggc tggcgcgctt
      541 caataccaat ctcgcggtgg tcgacaagcg ttttttccag ggcttgccca gccccgctgc
      601 ggcggcgctg gtggcgggct tcgtctggct gtcggtggat aacaagctgc ccatccatga
      661 ccacctcatg gcctggacga ccttcggcct gaccatgtat gcgggcatca ccatggtgtg
      721 cgacgcgccg ttcttcagcg gcaagaagtt cgcgctgggc cgcagcgttc ccttctgggg
      781 catcctgctg gtggtgctgg tgttcgtctt cgtgtccagc gacccgccgg tcatcctgtt
      841 cgtgctgttc gtgctgtacg gcctgtcggg ctgggtgacc tgggcctggc gctggagccg
      901 ggcgcgccgc ctgcagcagg agcgccgcgg cggggcgggt cagtagaagc cgagcctgct
      961 gcgctcgtag atggggtcgg ggccgtggaa gtgatgcgtg acatggtcgc ccttggggcc
     1021 catgaatata tgcatctgcg cattccagac gccttgttct ttatagcggt atgaccagac
     1081 cacttcccgc atgtcgccca ggcccacggt ctcgacctcg gagggcgggc cgaattcgca
     1141 gcggacctgg tccggcgtcc agtcgccggt gtccagcacc ttgaaatgct cgtcggtgag
     1201 cacggcctcc gcgcgctcga cgcggctgtc ggcgcccaca ttgacgcccc aggcgtactg
     1261 tcccaggggc tgcaaggtcc agaccgcccg ctgcccgccg tcggggcggg ggcagacgag
     1321 cttggggctg ccgtacctgg ccaggacggc ggcgtaggcg tcgccgggct ttacggcgcg
     1381 gtctatgttc tggcaggcgg acaggccggc aagcagggct gcggcggcgc cgcagcgaaa
     1441 taataggtga cgcataatgt ctcgacccgc tctgatttgg agggggtcga agctaagaat
     1501 gatccggccc cgcgtattag ggagattcta gactatcagc tataattgct gactgccgtc
     1561 tgtatgggcg gtaattttca aacccacgtc cgggccatgc cttttatagg cataacctca
     1621 gccctggcgc cagtccatag aggtcaataa tatgtctgtt gccgacatca acaaagccga
     1681 tatcgtttcg caattccaac gcgcccaagg cgataccggc tcccccgaag ttcaggtggc
     1741 tctgctcacc gcccgcatca acgagctgac cggtcacttc aaagaacaca tgaaggacca
     1801 ccattcgcgc cgcggtctgc tgcgcatggt cagccgccgc cgcaagctgc tcgactacct
     1861 caagggccgc aatcccgacg cgtatcgcgc tctgatcgaa aaactcggtc tgcgcaagtg
     1921 atcgacgggg caggctcccc ggggtcgaga gccccatgaa cataaccgtg gtcgatgcga
     1981 tcttgtcgca gcggccacgg ttttttaatt ttaggaataa tcagtcatgt tcaataaagt
     2041 gaccaagacg ttccagtatg gccagcacac ggttacgctg gaaactggcg agatcgcccg
     2101 ccaggcttcc ggcgccgtgg tggtctccat tgaagacacc gtcgtgctgg ccacggtggt
     2161 ggcggccaag aaggccaagc ccggccagga cttcttcccg ctgaccgtcg actacatcga
     2221 gaaaacctac gccgcgggcc gcatccccgg cggtttcttc aagcgcgaag gcaagccttc
     2281 cgaaaaagaa accctcacct cgcgcctgat cgaccgtccg ctgcgtccgc tcttccccga
     2341 gggtttctac aacgacgtgc aggtgatcat tcacacgctg tcggtcaacc ccgagatcga
     2401 tcccgacatc gccgccatga tcggctcgtc ggctgcgctg gccatctccg gcatcccctt
     2461 caacggcccc atcggcgccg tgcgcgtggg ctatgccgac ggccagtacg tgctcaaccc
     2521 cacggcctcg cagctcaagg actcgcagct caacctggtg gtcgccggca cggaaaccgc
     2581 cgtgctgatg gtcgaatccg aagcgaagca gctgtccgaa gaagtgatgc tgggcgccgt
     2641 ggtgtacggc catgagcaga tgcagaccgt catcaacgcc atccatgacc tggtccgcga
     2701 cgccggcaag cccgaatggg actggcagcc cgcagccaag aacgaagcgc tgatcgccgc
     2761 cgttgccgcc gccggccagg aaggcctggt tgccgcctac caggtccgcg aaaagcaggc
     2821 ccgcaccacc aagctgcgcg aagtctccgc aagcgtgaag gccaagctgg ccgagcaggc
     2881 cgcggctgcc ggccaggacg tcccctacgg cgtcaccgtt gacaacatcc tgttcgacat
     2941 ggaggcccgc atcgtgcgcg gccagatcct gaacggcgaa ccccgtatcg acggccgcga
     3001 cacccgcacg gtgcgcccca tcagcatccg cctgggcgtg ctgccccgcg cgcacggcag
     3061 cgccctgttc acccgcggcg aaacgcaggc cctggtggtc gccaccctgg gcaccaagca
     3121 ggacgagcag atcatcgatg cgctcatggg cgaataccgc gaccgcttca tgctccacta
     3181 caacatgccg cccttcgcca ctggcgaaac cggccgcgtg ggcgtgccca agcgccgcga
     3241 aatcggtcac ggccgcctcg ccaagcgcgc cctggcggcg ctgctgcccg cgcacgaaga
     3301 cttccagtac accatccgcc tggtgtcgga aatcaccgaa tccaacggct cctcgtcgat
     3361 ggcctcggtg tgcggcggct cgctcgccat gatggacgcc ggcgtgccgg tcaaggatca
     3421 cgtggccggc gtggccatgg gcctgatcct ggacgaaggc aagttcgccg tgctgaccga
     3481 catcctgggc gacgaagatc acctgggcga catggacttc aaggtggccg gcaccgtcaa
     3541 tggcgtgacc gccttgcaga tggacatcaa gatccagggc atcaccaagg aaatcatgca
     3601 ggtggcgctg gcccaggccc gcgaaggccg cctgcacatc ctcgacaaga tgcgcgaagc
     3661 gcaagacggc tcgcgcggcg agctgtcggc cttcgccccg cgcatgatct ccatgaagat
     3721 caaccccgag aagatccgcg acgtgatcgg caagggcggc gccaccatcc gcgcgctgac
     3781 cgaagaaacc ggcacgcaga tcgacatcca ggacgacggc accatcgtga tcgccagcgc
     3841 cgacgagaac aaggccaagg aagcccagcg ccgcatcgcc gagctgaccg ccgaggtcga
     3901 agtgggccag gtctacgaag gttcggtgct gcgcctgctg gacttcggcg ccatcgtgca
     3961 ggtgatcccc ggcaaggacg gcctgctgca catctccgag atcgccaact accgcatcgc
     4021 caacatcaac gatgtgctca aggtcggcca gcaggtccgc gtcaaggtca tcgaggccga
     4081 cgacaagggc cgcctgcgcc tgtcgatcaa ggcgctcggc ggtatcgaac agcagcaaca
     4141 gcccgaaacc gccccgcagg ccgaataagc ccgcaggttc gtttttcaaa agccccttta
     4201 aggggctttt ttgctgccga gccgcctcga ggcaaaaacg gccccctcgg ggggcagcaa
     4261 gcgcgtagtg cgcagcgtgg gggcgttttt ttgctgccga gccgcctcaa agcaaaaacg
     4321 gccccctcgg ggggcagcaa gcacgtagtg cgcagcgtgg gggcgtttct tgtccgccgg
     4381 cacctgcgcc aatgcctgcc gtttcggggt aaagtcaggg cacttcgcaa ccgaagaagg
     4441 tccgatgcat ggcaacaggg cggtggctgc tgcttggtgt atgggggatc ctgctgctgg
     4501 cagggtgttc atcgacgccg ccaggcaagg acggcggcac gccgaacggc gagtccatgt
     4561 cggtcgacca gatggtgcag accgacttca accgcaccgt caccatggcc atgcgcgaca
     4621 acctcaatgg cttgtacgtg ctgctcgaca agctctaccg ccgcaatccg cgcgaatggc
     4681 gcaagagcgg ccaggccgac gagaaagccg ccgtgcagcg ggtcaagacg ctgatcgagc
     4741 agcgccaacc gcccgcgggc ctggccggcc tgcgcgatgt ccagatcctg gccgtcgcgc
     4801 tcaaccccaa ttattcggac gaccgggtgg cggccttcgt ctacggcctg gccgacacca
     4861 tcattgccgc ccatggcgac aaggtgcgct tccatgccct cgacgtgctg gacggccagc
     4921 gcatctacaa cgcggcgcgc aatgtggagg ccgcggactg gctgctgtcc acgcgtcgtg
     4981 cgcccggggg cggccccttg ctgctgtcca atgagatggg ggacggcaag gtcaatttga
     5041 gttttgaacg ggaattcggc gccctcatcg gccgcctgga cctggaggcc aatcttctgg
     5101 gcgaaaatat acggcgcatc ggcatcaact atgcacaaag cctgatgttc ttcaatttcc
     5161 tgcccgtgcg ctgaccggaa tcgccatcgt gatcgatctt tccaccatac aagccgcccg
     5221 ccagcgcctg caggggcagg tgctcaagac gcccttcatg ctgtcgcgca cgctgtccga
     5281 catgctgggt gcgaaaatct ggctcaagtt cgagaatctc cagttcacgg cttcgttcaa
     5341 ggaacggggg gcgctcaacc gcatgctggc cctgtcgccg caagagcgcg ccaagggcgt
     5401 catcgccgta tcggcgggca accatgcgca aggggtggcc tatcacgcgc agcgcatggg
     5461 cgtgccggcc gtgatcgtca tgccgcgctt cacgcccacg gtcaaggtgg ccaacacgcg
     5521 gcgtttcggc gccgaggtgg tgctggcggg cgacacgttc gacgatgccc aggcgcatgg
     5581 ctatgaactc gtcgggcagc gcggcctggt catgatccat ccttacgacg acgaagccat
     5641 catcgcgggg cagggcacct tggcgctgga aatgctcgag gaccagcccg acctggacct
     5701 cctggtcatc gccatcggcg gcggcggact gatctcgggc gtcgccaccg cggccaaagg
     5761 catcaagccc ggcatccagg tcatcggcgt gcagaccgag cgcttccccg ccatgtatgc
     5821 ggccgtgcac gggcgcgaca tgcaaccggg gcaatacacc attgccgagg gcatcgcggt
     5881 gaagtcgccc gggcgcatca cccggaaaat cgtcggcgac ctggtcgaca gcatcgaact
     5941 ggtcagcgag gccgacatcg agcatgccat cgtggtgctg ctcgaaatcg agaaaaccgt
     6001 ggtcgaaggc gccggcgcgg ccggcctggc ggccttgctg agcgcccgcg cgcgagggca
     6061 gaccggctgg ttccagggca agcgcgtcgg cctggtgctg accggcggca atatcgatcc
     6121 gctcatgctg ggcgaactca tcgaacgcgg catggtgcgg gccggccggc tcgcccgcgt
     6181 gcgcgtgagc gcgcgcgacg tgccgggctc gctcgcccac atcacggcca ctgtggcaga
     6241 agcgggcgcc aacatcgacg aggtgcacca ccagcgcgcg ttcacgatgc tggcggcgca
     6301 gaacgtcgat gtcgagctcg tgttgcaaac gcgcggccgt acccatctcg ccagcgtgct
     6361 ggatgccttg cacgccgcgg gcttc
//
LOCUS       DASWPO010000329         5601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1865913, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000329 DASWPO010000000
VERSION     DASWPO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865913"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VGC69_17450"
     CDS             complement(<1..1316)
                     /locus_tag="VGC69_17450"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0297120.1"
                     /translation="MKRFAALYQELDRSTATLDKRAALAAYFREAPPRDAAWALYLLA
                     GSKITSARRKIAGTGELRAWAAAASHTPPWLVDASYDQVGDLAETLALLVPDPVQPAL
                     ERSLADWIEDVLLPLANRDEAERREVVVAAWLALRFDERLVFNKLLTGALRVGVSQRM
                     VQQALAQMSGVPIARIAQRMLGAWTPSPAFLSLLLSPEELPEDRRQPYPFFLASPLEG
                     EPASLGPLGDWLVEWKWDGIRAQLIRRHGEVALWSRGEERLDGRFPEVETAMAALGAD
                     CVLDGELLAWQDGAAGPMPFSALQTRIQRLKPGPRWLAEAPVRMLAYDLLELDGQDLR
                     ELPQRERRHRLEALLQRYPQPRLCLSPLVRSSGWEEAAALREESRERGVEGFMLKRAS
                     SPYQSGRRRGDWWKWKIDPLTIDAVLLYAQAGHGRRSTLYTDYTFGL"
     gene            complement(1340..2344)
                     /locus_tag="VGC69_17455"
     CDS             complement(1340..2344)
                     /locus_tag="VGC69_17455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEY0297121.1"
                     /translation="MPDLVQLRPEGLYCPDGDFFIDPWRPVPNAVVTHGHGDHARAGM
                     GCYHTSAEGLPILRWRLGRQDYRPRGYGEPFTLGKARVSLHPAGHVLGSAQVRIEAGG
                     RVWVVSGDYKRQPDPTCSPFEVVPCDTFITEATFGLPIYRWPATVDVAREIVQWRDRC
                     AARGEAAILYCYALGKAQRILAELAPLVDRPVYLHGAIAAGVDVYRQAGVAMADTRLV
                     IDAEGAAPAAGAGFAGELVLAPPSAAGSAWLRRFRKAQQGFASGWMRLRGNRRRRNMD
                     RGFVVSDHADWPDLLRTIHETGAARVIATHGDTHALVRTLNEAGVAAETLATQYGDEE
                     "
     gene            complement(2483..3988)
                     /locus_tag="VGC69_17460"
     CDS             complement(2483..3988)
                     /locus_tag="VGC69_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433335.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY0297122.1"
                     /translation="MTIGTMPALAVEGGYAGWRAQALRALAAGWPPEAVTWTERAGTA
                     GRGAQQMALDYAAPGPAPAPAGMAPAPPAGAGNAAPAVRISKGLAALLRDAALYRSPD
                     RWAFLYRVLWRWRHGDRAVASAADGDGARLNAMARAVRRARHDMIAYVRFRRREGAGI
                     PQYLAWYEPEHDVLADAAEHFSRRMGATTWCIGTPFGAALWNGQALQITDAPADARAM
                     RADTAADRIEPLWLLYYKSIFNPARLNETALHQRMPVRFWKGLPEGPLIPAMVAEARH
                     GARQVGQASAVGVMPGRRIAVDAPRAQPLRAAPTSLELCRRCELWRHATQPVPGQGPE
                     TARLMLIGEQPGDHEDLAGRAFIGPAGQVLDEALRRAGVPRDSLFLTNAVKHFKWTAR
                     GKRRMHKTPAQREVEACAHWLQEELARVRPAVIITLGATALGAVLGRTASLQDHLAAP
                     VRLGEAWLVATWHPSYALRADGTAARKEVIAGIAEAISLGRRLAADDSAAR"
     gene            complement(3985..5190)
                     /locus_tag="VGC69_17465"
     CDS             complement(3985..5190)
                     /locus_tag="VGC69_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HEY0297123.1"
                     /translation="MELSTKLEILADAAKYDASCASSGAPKRDSRGRDGLGASTGAGI
                     CHSFTPDGRCVSLLKILLTNFCQYDCQYCVNRRSSNVPRARFLPREVIDLTLDFYRRN
                     YIDGLFLSSGIIRSADYTMEQLVDVAQTLREAHSFRGYIHLKTIPDADPRLITLAGRY
                     ADRLSVNIELPTDGGLHRLAPEKDARTIKRTMGSIRLAQEEAREEKRRSAPAGQSTQM
                     IVGADEADDRTILQTAQALYGAYHLKRVYYSAFSPIPDSPSTLPAQPPPLLREHRLYQ
                     ADFLLRGYGFRAEELFQQAGDLPLDIDPKLAWALAHREAFPVDLNRAPQRLIARVPGI
                     GVRNAKRLVELRCLRAIRYQDLVRLRCAMEKVKPFIVVQDYRPGLAEPPSEQLRRALT
                     AAPVQLSLL"
BASE COUNT          878 a         1947 c         1912 g          864 t
ORIGIN      
        1 aggccgaagg tgtagtcggt gtagagcgtg ctgcggcgcc cgtggccggc ctgcgcatac
       61 agcagcaccg catcgatggt cagcgggtcg atcttccact tccaccagtc gccgcgccgc
      121 cggccgctct ggtagggcga gctggcgcgc ttgagcatga agccctccac gccgcgctcg
      181 cgcgattcct cgcgcagcgc ggcggcttct tcccagccgg aagaccgcac caggggcgac
      241 agacagagcc gcggctgagg ataccgctgc agcaacgctt ccagccggtg gcgtcgttcc
      301 cgctgcggca gttcgcgcag gtcttggcca tccaattcca gcaggtcgta cgccagcatc
      361 cgaaccggcg cctcggccag ccacctgggg ccgggtttca gccgctggat gcgggtctgc
      421 agcgccgaga acggcatcgg ccccgctgcg ccgtcctgcc aagccagcag ttcgccgtcc
      481 aatacgcagt ccgcgcccag ggccgccatc gcggtctcga cctcgggaaa gcgcccgtcc
      541 aggcgctctt cgccgcgcga ccacagcgcc acttccccgt gacgccgaat caactgcgca
      601 cgaatgccgt cccatttcca ttcgaccagc caatcgccga ggggacccag actcgccggc
      661 tcgccttcca gcggcgaggc caggaagaag ggataaggtt gccgacggtc ttccggaagt
      721 tcttccgggc tgagcagcag cgacaggaac gccgggctgg gcgtccacgc ccccagcatg
      781 cgctgggcga tgcgcgcgat cggcacgccc gacatctgcg ccagggcctg ctgcaccatg
      841 cgctgcgaca cgcccacccg cagcgcgccc gtcagcagct tattgaaaac cagccgctca
      901 tcgaaccgca aggcgagcca ggcagcgacg acgacctcgc ggcgctcggc ctcgtcgcga
      961 ttggccaagg gcaacaaaac gtcttcgatc cagtccgcca aactcctttc tagcgcaggc
     1021 tgcaccggat cgggcaccag aagtgccagg gtctcggcca gatcgcccac ttgatcgtag
     1081 ctggcatcga ccagccaggg cggggtgtgc gacgccgcgg cggcccaggc gcgcaattcg
     1141 cccgtgcccg cgatcttgcg gcgcgcgctg gtgatcttgc tgcccgccag caggtacagc
     1201 gcccatgccg catcgcgcgg cggcgcctcg cggaagtacg ccgccagcgc cgcgcgcttg
     1261 tccaaggtgg cggtggagcg gtccagttcc tggtacagcg ccgcgaatcg cttcatgtcc
     1321 ggggtcgcct ccttttaccc tattcttcgt cgccgtactg cgtggccagc gtctcggcgg
     1381 ccacgccggc ctcgttgagc gtgcgcacca gcgcatgggt atcgccgtgc gtggcgatca
     1441 cgcgcgccgc gccggtctcg tggatggtgc gcagcagatc cggccagtcc gcatggtcgg
     1501 agacgacgaa accgcggtcc atgttgcgcc gccgccggtt gccgcgcagc cgcatccagc
     1561 ccgaggcgaa accttgctgg gccttgcgga accgccgcag ccacgcgctg ccagccgccg
     1621 aggggggcgc caggaccagt tcgccggcga agcccgcgcc ggccgccggc gcggcgccct
     1681 cggcgtcgat gaccagccgc gtgtccgcca tggccacgcc cgcctgccgg tacacgtcga
     1741 ccccggcggc gatggcgccg tgcaggtaga ccggccggtc gacgagcggc gccagttccg
     1801 ccagtatccg ctgcgccttg cccagcgcat agcaatagag gatggcggcc tcgccgcgcg
     1861 cggcgcagcg gtcgcgccac tgcacgatct cacgcgccac gtcgacggtg gcgggccagc
     1921 ggtaaatcgg caggccgaag gtcgcttcag tgatgaaagt gtcgcagggc acgacttcga
     1981 aggggctgca ggtaggatcg ggctggcgct tgtagtcgcc ggacaccacc catactcggc
     2041 ctcctgcctc gatgcgtacc tgggccgaac ccagcacatg gccggcgggg tgcagggaca
     2101 cgcgagcctt gcccagggtg aagggttcgc cgtagccgcg cgggcgatag tcctgcctgc
     2161 ccagccgcca tcgcaggatg ggcaggccct cggcgctggt gtggtagcag cccatgcctg
     2221 cccgggcatg gtccccgtgg ccgtgcgtca ccaccgcgtt gggcacaggc cgccacggat
     2281 cgataaagaa atcgccatcc gggcagtaca ggccttcggg gcgaagttgc acgaggtcag
     2341 gcatggcggg ctcccgcgtc cagcggcgca tcgcgcatcg aggacgtctg agaatggtta
     2401 taccccgatg tatgaaacgg acaggtatct gtttcttgca tgcggcgatc atccgcaacg
     2461 gcggcgcgcg gacgtcttcc ccttatcgtg ccgccgagtc atccgccgct agccgccgac
     2521 ccagcgagat ggcctcggcg atgccggcaa tgacttcctt gcgcgccgcc gtgccgtccg
     2581 cacgcagcgc gtacgaggga tgccaagtgg cgaccagcca ggcctcgccc agccgcaccg
     2641 gcgcggccag atgatcctgc aggctggcgg tgcgtcccag gacggcgcct agcgcggtgg
     2701 cgcccagggt gatgatgacg gcgggccgca cgcgcgccag ctcctcttgc aaccaatgcg
     2761 cgcaggcctc gacctcgcgc tgcgccggcg tcttgtgcat gcgccgcttg ccgcgcgcgg
     2821 tccacttgaa gtgcttgacc gcgttggtca gaaagagcga gtcgcgcggc acgccggcgc
     2881 ggcgcagcgc ctcgtccagc acctggccgg ccgggccgat gaaggcgcgg ccggccaggt
     2941 cttcatggtc gccgggctgt tcgccgatca gcatcaggcg cgccgtctcg gggccttggc
     3001 ccggcacggg ctgcgtggcg tggcgccaga gctcgcagcg gcggcacagt tcgagcgacg
     3061 tcggggctgc gcgcagcggc tgcgcacgcg gcgcgtcgac cgcgatccgc cgaccgggca
     3121 tgactcccac ggcgctggcc tgcccaactt ggcgcgcgcc atggcgcgcc tcggcgacca
     3181 tggcgggtat cagcggcccc tcgggcaggc ctttccagaa gcgcaccggc atgcgctggt
     3241 gcaacgcggt ttcgttcagg cgggcgggat tgaagatgct cttgtaatag agcagccaca
     3301 gcggctcgat ccggtcagcc gcggtgtcgg ctcgcatcgc ccgggcatcg gcgggcgcgt
     3361 ccgtgatctg taatgcctgg ccgttccaca gggccgcgcc aaacggggtg ccgatacacc
     3421 atgtcgtggc gcccatacgc cgggagaaat gttccgccgc atcggccagc acgtcgtgct
     3481 cgggttcgta ccaggccaga tactgcggta tgccggcgcc ctcgcgccga cggaagcgca
     3541 cataggcaat catgtcgtgc ctggcgcgcc gcacggccct ggccatggcg ttcagccgcg
     3601 cgccgtcccc gtcggcggcc gacgccacgg cgcggtcgcc atgccgccag cgccacagca
     3661 cgcggtacag aaaggcccag cggtccggcg aacggtacag cgcggcgtcg cgcagcaagg
     3721 cggcgaggcc cttcgagatg cgcacggcgg gcgcggcgtt cccggcgccg gccggcggcg
     3781 ctggcgccat gccggccggc gcaggcgcgg gtccaggcgc ggcatagtcc agcgccattt
     3841 gctgcgcgcc gcgacccgcc gtgccggcgc gctcggtcca ggtcaccgcc tcgggcggcc
     3901 agccggccgc cagcgcccgc agcgcctgcg cgcgccagcc cgcatagccg ccttcgaccg
     3961 ccagcgcggg catagtgccg atagtcatag caaggacagc tgcaccggcg cagccgtcag
     4021 cgcacgccgc aattgttccg aagggggttc ggccaggccg ggccggtagt cctgcacgac
     4081 gatgaagggc ttgaccttct ccatggcgca gcgcagacgc accaggtctt gatagcggat
     4141 agcacgcagg cagcgcagtt ccaccagccg tttggcattg cgcacgccga tgcccggcac
     4201 gcgcgcgatc aggcgctgcg gcgcacggtt caggtcgacc gggaaggcct cgcgatgcgc
     4261 cagcgcccag gccagcttgg gatcgatatc cagcggcagg tcgcccgcct gctggaacag
     4321 ttcctcagcc cggaacccat agccgcgcag caggaagtcc gcctggtaca ggcgatgctc
     4381 gcgcaacagc ggcggcggct gcgcgggcag cgtcgagggg ctgtcgggta tggggctgaa
     4441 cgcggagtaa tacacacgct tgaggtggta cgcgccgtac agcgcctggg ctgtctgcag
     4501 gatggtgcgg tcgtcggcct cgtccgcgcc cacgatcatc tgcgtgcttt gtcccgccgg
     4561 ggcgctgcgc cgcttctctt cgcgcgcctc ttcttgcgcc aaccggattg accccatggt
     4621 ccgcttgatg gtgcgtgcgt ctttttcagg cgccaggcga tgcaggccgc cgtcggtagg
     4681 gagttcgatg ttcacgctga gccgatcggc gtaccggccc gcgagggtga tcagccgtgg
     4741 atcggcatcg gggatggtct tgaggtgaat gtagccgcga aagctgtgcg cctcgcgcag
     4801 cgtctgtgct acgtcgacca actgttccat ggtgtagtcg gcgctgcgga taatgccgga
     4861 actgaggaac aagccatcga tatagttgcg gcgataaaag tccagcgtca gatcgatgac
     4921 ttcgcggggc aagaatcgtg ctcgcgggac attgctggag cgccggttta cgcaatattg
     4981 gcagtcgtac tggcagaagt tggtcaatag gatcttcagc agggacacgc aacgcccgtc
     5041 tggggtgaag ctgtggcaga tgccggcgcc cgtactggcg cccaacccgt cgcgtccacg
     5101 cgagtcccgc ttgggggcac cgctgctggc gcatgaagcg tcgtatttcg ccgcatccgc
     5161 caggatctca agtttggtgg atagctccat gtgcccaagt actgtatttt tatacagtat
     5221 tataggtgcg gctacgaaaa tggcaaatgt gcctgttatc ggcctgcccc atcaaggcag
     5281 caaccatatg gactcaccgt cacaagcgcg gcagcaccta ctatttctgc cgcgccgtcc
     5341 cgcacccacg gtatagccga agggcatggc ccgcaggatg cgccggtagt cgctgctggc
     5401 aagagttccc gccgaacagc cgctcaccca tgaacgtgtc ccgcgtggcg tcatccatgc
     5461 cggcatggat ggagcaactt agagcatcac gccatcctga ggcaccaggg ggcgtggcag
     5521 attggtgtcg ccgagcagcc ctcccacgct gatctcgacg ttgcgacaga gcgcgtccag
     5581 cggcaagttg ttcggcagca g
//
LOCUS       DASWPO010000330         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2847616, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000330 DASWPO010000000
VERSION     DASWPO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847616"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1389
                     /locus_tag="VGC69_17470"
                     /pseudo
     CDS             1..1389
                     /locus_tag="VGC69_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063702.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
     gene            1602..2198
                     /locus_tag="VGC69_17475"
     CDS             1602..2198
                     /locus_tag="VGC69_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEY0297124.1"
                     /translation="MAYSRQLFRRTGLAAATVAFAGLAFTGCTTNSQKDTGSVSNQRS
                     EINAGADSTLSKLYQSTSQAQSLVSQAKGVLIFPSVISASFIVGGEYGKGVLREGGKP
                     VAYYSTASGSLGFQAGAQSRATVLLFMTDDALAAFKKSQGWTVGADATVAIAKIGANG
                     SIDTNTVKQPVVGFVLNNAGLMAGVSIDGTKITRDTAL"
     gene            complement(2350..2958)
                     /locus_tag="VGC69_17480"
     CDS             complement(2350..2958)
                     /locus_tag="VGC69_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0297125.1"
                     /translation="MKTLVIKSSILGENSKTKVLVDHFTARIKQADPAGTITVRDLGA
                     QPVPYFDGATIGALFTPADARSAAQKEVVAFSDSLVAELFDADRIVFAVPIYNFSLPA
                     QLKSYIDYVSRAGVTFRYTPEGVPEGLVTGKEVIVLGARGGKAEGTPHDTLTPYLTQI
                     LGFLGMTGITFVAAEGMAMGPESATAGLEQAKARIDALAIKA"
     gene            3095..4030
                     /locus_tag="VGC69_17485"
     CDS             3095..4030
                     /locus_tag="VGC69_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0297126.1"
                     /translation="MQDLNDLYYFAQVVEQGGFSAAARMLDIPKSRLSRRISQLEERL
                     GVRLLQRTTRRLRLTTAGERYLRYCREMTASARAAEDAMRQLQSEPSGPVVISCPVSL
                     AQQILAPLLPEFLDTWPAVSVQMLVTNRRVDLIREGVDLALRVRTRLDTDAELVVRRF
                     GTAMSTLVASPAYLQRHGMPESPQDLVNHTTLSFNDPQPEVHWTLRNLQGEEMEVTVQ
                     PQLCCNDFIMLTQAAVRGRGVALLPDEAVREEMRRGQLVRVLPDWRSPEGILHCIYPS
                     RRGMMPAVRALLDFLSERLPPAYRHVDMPPESQAA"
     gene            complement(4050..>5257)
                     /locus_tag="VGC69_17490"
     CDS             complement(4050..>5257)
                     /locus_tag="VGC69_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY0297127.1"
                     /translation="GGTALGITMICSFFAASFGILVMIFASPLLVEIAFKFGPTEIFS
                     IMLLGLLAGATMSRGSPLKGVAMTLFGLLAGVAGTDVNTGQIRFSFGLVDMSDGLELV
                     AVSMGLFGVADFLMSANRMASINTSTTNLRIRDMRPSLSELKQAFFPMVRGTLVGTIF
                     GAMPGTGPTITTFISYALERKLSKTPEKFGTGMIAGVAGPEASSHSKTQVDFIPTMSL
                     GIPGDAVMALILGALLIQGIQPGPQLITEHPDIFWGLIASFWVGNVLLMVLNVPMIGV
                     WVKMLQVPYRYLFPSALFFIAVGVFSTQNNLFQIWEVLAFGIIGALFMTLEFSVAPIL
                     LGFVLGPMVEENFRRALLLSRGDMGIFIQRPISAWFVAASALLIVLQVGAFVRKVLRE
                     RKAAATQTA"
BASE COUNT          954 a         1673 c         1724 g          906 t
ORIGIN      
        1 ggcccggtgg cgaccatggc gatcctgctg ccgatcacct tctacgtgcc gccggcgggc
       61 gccctgatca tgctggccgg catttactat ggcgctcaat atggcggctc gaccacctcg
      121 atcctgatga acatgcccgg agaggcggca agcgtcgtca cctgcctcga cggccaccag
      181 atggcgcgca acggccgggc gggcgcggcg ctggccattg ccgcgttggg ctcgttcttt
      241 gccggcagcg tggccacgct gctcctggcc gcctttgcgc cgccgctggc cgaggttgcc
      301 ttcaagttcg gcccggccta atatttctcg ctgatggtgc tgggcctggt gggcgcggtc
      361 gtgctggcct cgggctcgct gcccaaggcc atcgcgatga tcctcctggg cctgctgctg
      421 ggcatggtgg gcaccgatgt caactcgggc gtggcgcgct tcgatttcgg cattgccgag
      481 atgcaggacg gcatcgactt cgccatcgtg gccatgggcg tgttcggctt cgccgagatc
      541 atgaacaacc tggagcagaa ggaaaaccgc gtggatgtca ccgacaaggt gggcacgctg
      601 tttcccaaca agcaggagtt caaggaggcc taccccgccg tgctgcgcgg cacggcgctg
      661 ggctcggcgc tgggcatcct gccgggcggc ggcgcggtgc tgtcgtcctt cgcctcatat
      721 acgctggaaa agaagatcac ccgcgagccg cagcgcttcg gcaagggcca ccccgccggc
      781 ctggccggcc ccgagtcggc caacaatgcc gccgcgcaga cttcgttcat tccgctgctg
      841 acgctgggca tacccggcaa cgcggtgatg gcgctgatgg tcggcgccat gaccatccac
      901 agcatccagc ccggcccgca agtcatgacc agccatcccg acctcttctg gggactgatc
      961 gcctcgatgt ggattggcaa cctgatgctg gtgatactga acctgccgct gatcggcctg
     1021 tggatcaagc tgctgaaggt gccctaccgc gtgctgttcc cggcgatcct ggtgttctgc
     1081 acgatcggcg tgtattcgct gaactacaac accttcgaca tcctgatgac ggccggcttc
     1141 ggtctggtgg gctatctgtg gtccaagctc aagtgcgagg gcgcgccgct gctgctgggc
     1201 ctggtgctgg gccccatgat ggaagaaaac ttccgccgcg ccctgctgct gtcgcgcggc
     1261 gactatgcca ccttcgtcac gcgtccgctg tcggcgtcgt tgctggccgt tgtcgtggtg
     1321 ctggtgatcg tggtggccct gccctcgatc cgcaagaagc gcgaagagac gttcgttgaa
     1381 gaggagtgat ccgcgccagc cgcgcgcaaa acaagcgcgc agtccgtcga tagccaccag
     1441 cccttcccgg cggatatctg gcgccctctc cagccgcctc agcgcggctt tccataccta
     1501 tcgcccgggg aggggctttc tttcctttta gaacatccgt tcacaatcgt caggaaagga
     1561 aagggtaaat tgataaagcc agaacaacca aggagaaaat catggcgtac tcacgacagc
     1621 ttttccggcg tacgggtctt gccgccgcga ccgtggcatt tgccggcctt gcctttacgg
     1681 gctgcaccac gaattcccag aaagatacgg gcagcgtttc caaccagcgc tcggaaatca
     1741 atgccggggc cgattcgacc ttgtccaagc tgtatcaatc aacgtcccag gcacagagcc
     1801 ttgtcagcca ggccaagggg gttttgatct tcccctccgt catcagcgcc agcttcatcg
     1861 tgggcggcga atacggcaaa ggcgtcttgc gcgaaggcgg caagcctgtt gcctactatt
     1921 ccaccgcatc gggttcgctg ggctttcagg ccggcgcgca gtctcgcgcc acggtgctgc
     1981 ttttcatgac ggacgacgcc ctggccgcgt tcaagaaaag ccagggatgg acggtgggcg
     2041 ccgacgccac ggtggcgatc gccaagatcg gagccaacgg cagcatcgat acgaacaccg
     2101 tgaaacagcc tgtcgtaggc ttcgtcctca ataatgcggg gctgatggcc ggcgtctcca
     2161 tcgacgggac caagatcacg cgcgataccg cgctgtagcc accaggcaag ccccaggacg
     2221 cggcccgcgc tttccaaaag cgctccgggc ccgcaaacaa acacccccga aggccgggat
     2281 tttgcccagc cctcgggggt gttttcatgc ctggacggca aacggcagaa accgctgcct
     2341 gaaaggcttt caggccttga tcgccagggc gtcgatacgc gccttggcct gctccaggcc
     2401 ggccgtggcg gactcggggc ccatggccat gccttcggcg gcgacgaagg taatgccggt
     2461 catgcccagg aagcccagga tctgggtcag gtagggggtc agcgtgtcgt ggggggtgcc
     2521 ttcggccttg ccgccgcggg cgcccagcac gatgacttcc ttgcccgtga ccaagccttc
     2581 gggcacgccc tcgggggtgt agcggaaggt cacgccggcg cgggagacgt agtcgatata
     2641 gctcttgagc tgggccggca ggctgaagtt gtagatcggc acggcgaaca cgatgcgatc
     2701 ggcatcgaac agttcggcga ccaggctgtc gctgaaggcc acgacttcct tctgggcggc
     2761 agaacgggca tcggcgggcg tgaagagcgc accgatggtg gcgccgtcaa aatacgggac
     2821 aggctgggcg cccaagtcac gcacggtgat cgtgccggcg ggatccgcct gcttgatcct
     2881 ggcggtgaag tggtcgacca aaaccttggt cttcgagttc tcgcccagga tcgacgattt
     2941 gatgacgagg gtcttcatga atatctccgg aaagggaggc ggtagctcgc cgcctgaaca
     3001 tgaccgacac tctaaagcag ctgattattt gcataaatct gattaagatg aactctaagt
     3061 tccaaaaata gaacagtccg gagtcgaatc cgtcatgcaa gacctcaatg acctgtacta
     3121 cttcgcccaa gtcgtggaac aaggcgggtt cagcgccgcg gcacgcatgc tggatatacc
     3181 caagtcgcgc ctgtcgcgcc ggatttcgca actggaagag cggctgggcg tgcggctgct
     3241 gcagcgcacg acgcggcgcc tgcgcctgac gacggccggt gagcgctacc tgcgctactg
     3301 ccgggaaatg accgcctcgg cgcgcgccgc cgaagatgcc atgcggcagc tccaatccga
     3361 gccctcgggc ccggtggtga tcagttgccc ggtatccctg gcccagcaga tcctggcgcc
     3421 gctcttgccc gaattcctgg atacctggcc ggccgtctcg gtgcagatgc tggtgaccaa
     3481 tcggcgggtc gatctgatcc gcgaaggcgt ggacctggcg ctacgcgtgc gcacacgcct
     3541 ggacaccgac gccgagctgg tggtgcggcg cttcggcacg gccatgagca cgctggttgc
     3601 cagcccggcc tatctgcagc gccatggcat gccggaatcg ccccaggacc tggtgaacca
     3661 caccacgctc agcttcaacg acccgcagcc cgaggtgcac tggacgctgc gcaatctgca
     3721 gggcgaagaa atggaagtga cggttcagcc gcagctgtgc tgcaacgact tcatcatgct
     3781 gacgcaggcg gcggtacgcg gccgaggcgt ggcattgctg cccgatgaag ccgtgcgtga
     3841 ggagatgcgc cgcggccagt tggtgcgggt gctgccggac tggcgctcgc ctgaggggat
     3901 actgcactgc atctacccct cgaggcgggg catgatgccg gcggtgcggg cgctgctgga
     3961 tttcctgtcg gaacgcctgc cgcccgccta ccgccacgtc gacatgccgc ctgaaagcca
     4021 ggcggcatga accgctgaac cccggaggcc taggccgtct gggtggcagc cgccttgcgc
     4081 tcgcgcagca ccttgcgcac gaaggcgcca acctgcagca cgatcagcag cgcgctggcc
     4141 gccacgaacc aggcgctgat gggacgctgg atgaagatcc ccatgtcgcc gcgcgacagc
     4201 agcagcgcgc ggcggaagtt ttcttcgacc atggggccca gcacgaagcc cagcaggatc
     4261 ggggccaccg aaaactccaa ggtcatgaag agcgcgccga tgatgccgaa ggccagcact
     4321 tcccagatct ggaagaggtt gttctgcgtg ctgaacacac ccaccgcgat gaagaacagg
     4381 gccgacggga acaggtagcg gtacggtacc tgcagcatct tcacccacac gccgatcatc
     4441 ggcacgttga gcaccatcag cagcacgttg cccacccaga agctggcgat caggccccag
     4501 aagatgtccg ggtgctcggt gatcagctgc ggaccgggct ggatgccctg gatcagcagc
     4561 gcgcccagga tcagcgccat caccgcatcg ccggggatgc ccaggctcat ggtggggatg
     4621 aaatcgacct gcgtcttgga gtgcgaggag gcttcgggcc cggccacgcc ggcgatcatg
     4681 ccggtgccga acttctcggg cgtcttggac agcttgcgct cgagcgcgta cgagatgaag
     4741 gtggtgatgg tggggccggt gcccggcatg gcgccgaaga tggtcccgac cagcgtgccg
     4801 cgcaccatgg gaaagaaggc ctgcttcagc tcggacaggc tgggccgcat gtcgcggatg
     4861 cgcaggttgg tggtgctggt gttgatcgag gccatgcggt tggcgctcat caggaagtcg
     4921 gccacgccga acaggcccat tgacaccgcc accagttcca ggccgtcgga catgtcgacc
     4981 aggccgaagg aaaagcggat ctgcccggtg ttgacgtcgg tgccggccac gccggctagc
     5041 aggccgaaca gcgtcatggc cacgcccttg agcggcgaac cgcgcgacat ggtggcgccg
     5101 gccagcaggc ccagcagcat gatggagaag atctcggtgg ggccgaactt gaaggcgatc
     5161 tcgaccagca gcggcgaggc gaagatcatc accaggatgc cgaacgacgc cgcgaagaac
     5221 gagcagatca tcgtgatgcc cagcgccgtg ccgccct
//
LOCUS       DASWPO010000331         6282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2670606, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000331 DASWPO010000000
VERSION     DASWPO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670606"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /gene="hutC"
                     /locus_tag="VGC69_17495"
     CDS             complement(<1..676)
                     /gene="hutC"
                     /locus_tag="VGC69_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEY0297128.1"
                     /translation="MTSIQASSGSVALYQQVKDFIVRNIMSGTWPAGYRLPSEHELVA
                     QFGISRMTVNRALRELSAQGQVERIAGVGSFVAREKPQSTLLHIANLAEEIRRRGHDY
                     HCDVLVSERVSAPLDISTALDLDIGMSVFHTLCVHREDGVPVQLEDRYVNPLAAPQFG
                     QQDFTQVQPSEYLLRNVPCDQIEHVIDAVLPTADQARQLEMESAQPCLLLTRRTWTQS
                     TPVTLVR"
     gene            866..2545
                     /gene="atzF"
                     /locus_tag="VGC69_17500"
     CDS             866..2545
                     /gene="atzF"
                     /locus_tag="VGC69_17500"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEY0297129.1"
                     /translation="MPKHSTATPAAAWIDKFPQALSGAAQGPLAGLRFAAKDNIDVAG
                     VPTTAACPAYATTPAAHAAVVARLLAAGAGLAGKTNLDQFACGLNGTRSPYGAVPNAF
                     DAAYVSGGSSSGSAYAVARGEVDFALGTDTAGSGRVPAGLNNIVGLKPSKGLVSMHGV
                     LPAAQSVDCVSIFARTVAMAVRVLEVAMGYDARDPYSRPMALAAQALPARFRFGVPAA
                     PEFFGDALARAAFGLAVEQLRALGGTPVPIDYTPLAEAAGLLYESALVAERYAAIRDF
                     FDASPDAVIEPVRTIVGAGTRYAAADVFDAQHRLQALGQQARALWQGMDILCVPTAPT
                     HCTIDAMLAEPVLRNRELGYYTNFVNLLDYAALSVPASLRPDGLPFGVTLIGQAGSDW
                     QLAELGQRLHHASGLTQGATGEPLPEPEPVAALRPTAATHLPLVVVGAHLSGLSLNGQ
                     LTELGASLAEAVRTAPRYRLYALPGAAPRRPGLRRVGEGGASIAAEVWQVPLAAVGRF
                     LAQVPPPLGLGSVEVADGRWLHGFICEGHAFDGAEDVSRHGGWRAYLASMP"
     gene            2617..3633
                     /locus_tag="VGC69_17505"
     CDS             2617..3633
                     /locus_tag="VGC69_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297130.1"
                     /translation="MSKVPSSPASLLSSAGRRRALRSAAGLFGVLAAPALVRAQTLPK
                     IRIGFWPVAAGLPFFAAVDKGYFKEAGLDVEPLKFASAQLVIEAILAGRAEGSANGTA
                     SAALGLGEIAQPGLLKIFCANPTNQKFVLDEFLVAKDSPIQSIAELKGKRVASGPGVQ
                     NVVLAKTVLERAGAGKMTVTELPIGQHVAALAAGQIDAVYTLEPTGTVGRMNGTTRAL
                     EVGVIAKYVLGDPMAPWFGGSASLTTDFIKQSPDVARKFIAAYKRGVDLVQQKPDEAR
                     PSLKGYTAIEGALTAEVPMSDYLFSGDFKPSDVAYFQKFFDLFSDNGIFSKRLMVESM
                     LYKA"
     gene            3639..4472
                     /locus_tag="VGC69_17510"
     CDS             3639..4472
                     /locus_tag="VGC69_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297131.1"
                     /translation="MDKTPASTPTASGWSPPVSRDAAPARAPLRDRMLPFVGPVVLFI
                     IWDLVVRFGLIKAILLPSPLDTLIALARGLAGGDLLTDFLVTVGRTLESFVIAAVVGM
                     PLGVLLGSNERAYRSVEFLIDFFRSTPSSALIPLFLLIFGVSDINKVAIAAFGALLIV
                     VFNSAYGVINARKQRVMAAKVMGASRWRIFKDVLVWESLQPSFVGLRSAVSMALVIVI
                     VAEMFIGSENGLGHAIIDAQQVLNVKTMYAAILAAGALGYVLNILFLMLERRVVHWSG
                     R"
     gene            4472..5293
                     /locus_tag="VGC69_17515"
     CDS             4472..5293
                     /locus_tag="VGC69_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297132.1"
                     /translation="MTTVLNVPAYADVPAPRFVPGPTGTHITIRGLTKYFAGWPLYEN
                     FDLDIPKNRIVSVFGPNGCGKSTLINMIAGLVPIDHGEILFDGKSLKDTRIGYVFQNY
                     REALFPWMRTIDNIAYPLLLEGKSKTEVDRRMQELVASFDVKFDLKRYPYELSGGQQQ
                     TASIMRALAPRPEVLFLDEPFSALDFEMTLFIREKLQEVFMQTGTTMMLVSHDLEEAV
                     YLADQVLLLTKRPTRVAEILNYGDARPRTVETLSQPGFIETKKLSLEIFQREVRR"
     gene            5290..5499
                     /locus_tag="VGC69_17520"
     CDS             5290..5499
                     /locus_tag="VGC69_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4089 domain-containing protein"
                     /protein_id="HEY0297133.1"
                     /translation="MSADTVLHFVRASATLLGLPLDDAQAGRVAAHMARTAGMARLLE
                     AAPLAEDDEPAEVYRPAPFPEAPGA"
     gene            5542..>6282
                     /locus_tag="VGC69_17525"
     CDS             5542..>6282
                     /locus_tag="VGC69_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0297134.1"
                     /translation="MNFPEISPGHGLDRLNAVARGEMTARAWLEDCVARVETTDGRVN
                     AYTLRTFERARREADAVDALRAAGQPLPPLAGLPYAVKNLFDIEGEVTLAGSKVNRSH
                     APAAADAVLVQRLRAAGAVLVGALNMDEYAYGFTTENTHYGPTCNPHDPARIAGGSSG
                     GSGATVAAGQVPAALGSDTNGSIRVPASLCGVWGLKPTFGRLSRRGSYPFVHSIDHLG
                     PFADSAGMLARVYDALQFPDARDPGCHAR"
BASE COUNT          917 a         2145 c         2150 g         1070 t
ORIGIN      
        1 agcgcacgag cgtgacgggg gtgctttgcg tccaggtgcg gcgggtgagc agcaggcagg
       61 gctgggccga ctccatctcg agctggcggg cctggtcggc ggtgggcagg acggcgtcga
      121 tgacgtgctc gatctggtca cagggcacgt tgcgcagcag gtattcggag ggctggacct
      181 gggtgaagtc ctgctggccg aactgcggcg cggccagcgg gttgacgtag cggtcttcca
      241 gctgcacggg cacgccgtcc tcgcgatgca cgcacagggt gtggaagacc gacatgccga
      301 tgtccaggtc gagcgcggtg gagatgtcca gcggcgcgga gacgcgctcg ctcaccagca
      361 cgtcgcagtg gtagtcatgg ccgcggcggc ggatttcttc agccaggttg gcgatgtgca
      421 gcagggtgga ctgcggcttt tcgcgcgcca cgaagctgcc caccccggcg atgcgctcga
      481 cctggccctg ggccgacagc tcgcgcaggg cgcggttgac cgtcatgcgc gagatgccga
      541 actgcgcgac cagttcgtgc tcggacggca gccggtaccc ggccggccag gtgccgctca
      601 tgatgttgcg cacgatgaaa tccttgactt gctggtacag ggccacggag ccggaagagg
      661 cctggatgct ggtcatgaat cgggacggta tcggggttgc gtgtctgagg ctggggcgcc
      721 gggcggggga tgcccgccag gacggcgcct tggatgacct gattgtcaca tggtggtcta
      781 tacaactggc ctggggtatt cccggctttc tgtctcggcc ggctttcttt atcttgtgta
      841 caagtttgga ttttacagaa gaatcatgcc caagcattcc accgctaccc cggcggccgc
      901 gtggatcgac aaattcccgc aagccctgtc cggcgctgcg caaggcccgc tggccggcct
      961 gcgctttgcc gccaaggaca atatcgacgt ggccggcgtg cccaccacgg cggcgtgtcc
     1021 cgcctacgcc accacgcccg cggcgcacgc ggccgtggtg gcgcggctgc tcgcggccgg
     1081 cgccggcctg gcgggcaaga ccaacctgga ccagttcgcc tgcggcctga acggcacgcg
     1141 ttcgccctac ggcgccgtgc ccaacgcctt cgacgcggct tatgtcagcg gcgggtcgag
     1201 ttccggttcg gcgtatgcgg tcgcgcgcgg cgaagtcgat ttcgccttgg gcaccgacac
     1261 ggcgggttcg ggccgggtgc ccgcgggctt gaacaatatc gtgggcctca agcccagcaa
     1321 gggactggtt tcgatgcatg gcgtgctgcc ggcggcgcag agcgtggatt gcgtctccat
     1381 cttcgcgcgc acggtggcca tggcggtccg ggtgctggaa gtggccatgg gctacgacgc
     1441 gcgcgatccg tattcgcgcc cgatggcgtt ggcggcgcag gcgctgccgg cgcgttttcg
     1501 cttcggcgtg ccggcggcgc ccgaattctt cggcgatgcg ctggcgcggg cggcgttcgg
     1561 cctggccgtc gagcagttgc gggcgctggg cggcacgccc gtgccgatcg actacacgcc
     1621 gctggccgag gcggccggcc tgctctatga aagcgccctg gtggccgaac gctatgccgc
     1681 catccgcgac ttcttcgatg cgtcgcccga tgcggtgatc gaaccggtgc gcaccatcgt
     1741 gggcgccggc acgcgctatg cggccgccga tgtcttcgac gcgcagcatc ggctgcaggc
     1801 cctgggccag caggcccgcg cgctgtggca gggcatggac atcctgtgcg tgcccaccgc
     1861 gcccacgcat tgcacgatag acgccatgct ggccgagccg gtgctgcgca atcgcgagct
     1921 cggctactac accaatttcg tcaacctgct cgattatgcc gcgctgtcgg tgcccgcgag
     1981 cctgcggccc gacggcctgc ctttcggcgt cacgctgatc ggccaggccg gcagcgactg
     2041 gcaactggcc gagctgggcc agcgcctgca tcacgccagc gggctgacgc agggcgccac
     2101 gggcgaaccg ctgcccgaac ccgaacccgt cgccgccctg cggcccacgg ccgccacgca
     2161 cctgccgctg gtcgtggtgg gggcgcacct gtcgggcctg tcgctcaacg gccaactcac
     2221 cgaactgggc gcgagcctgg ccgaagcggt gcggaccgcg ccgcgctacc ggctgtatgc
     2281 gctgcccggc gccgcgccgc gccggccggg gctgcggcgg gtgggcgagg gcggcgcgtc
     2341 catcgcggcc gaggtctggc aggtgccgct tgccgccgtg ggacgcttcc tggcccaggt
     2401 gccgccgccc ctggggctgg gcagcgtcga ggtggccgac ggccgctggc tgcacggttt
     2461 catctgcgaa gggcatgcgt tcgacggcgc cgaggacgtc agccgccacg gcggctggcg
     2521 cgcctacctg gccagcatgc cctgaatcgt ctttgctgta ctgcattccg cttttgtccg
     2581 tgccttcgac cctcaatcga catccaggag ttcgccatga gtaaagtgcc ttcttccccc
     2641 gcttccctgc tatcgagcgc cggccgccgg cgcgccttgc gctcggccgc cggcctgttc
     2701 ggcgtgctgg ccgcgcccgc gctggtgcgg gcgcagacct tgcccaagat ccgcatcggc
     2761 ttctggccgg tggccgcggg cctgcctttc ttcgcggcgg tggacaaggg ttatttcaag
     2821 gaggccgggc tggacgtcga accgctgaag ttcgccagcg cccagctggt gatcgaggcc
     2881 atcctggccg gccgcgccga aggcagcgcc aacggcacgg cctcggcggc gctggggctc
     2941 ggcgagatcg cccagcccgg cctgctgaag attttctgcg ccaacccgac gaaccagaaa
     3001 ttcgtgctcg atgagttcct cgtcgccaag gacagcccga tccaatccat cgccgagctc
     3061 aagggcaagc gcgtggcctc gggcccgggc gtgcagaacg tggtgctggc caagaccgtg
     3121 ctggagcgcg ccggcgccgg caagatgacc gtgaccgaac tgcccatcgg ccagcacgtg
     3181 gcggcgctgg ccgcgggcca gatcgacgcg gtctatacgc tggagcccac cggcaccgtg
     3241 gggcgcatga acggaaccac gcgcgcgctg gaagtcggcg tgatcgccaa gtatgtgctg
     3301 ggcgatccca tggcgccctg gttcggcggc tcggccagcc tgacgacgga cttcatcaag
     3361 caatcccccg atgtcgcgcg gaaattcatc gccgcctaca agcgcggcgt cgatctcgtg
     3421 cagcaaaagc ccgacgaggc gcggccctcg ctcaagggct acaccgcgat cgagggggcg
     3481 ctgacggccg aggtgccgat gtcggactat ttgttctccg gcgatttcaa gcccagcgac
     3541 gtggcctact tccagaaatt cttcgacctg ttctccgaca acggcatttt ttccaagcgc
     3601 ctcatggtcg aatccatgct ctacaaggcc tgactcccat ggataagaca cctgcttcca
     3661 cgcccacggc cagcggctgg tcgccgccgg tttcccggga tgcggcgccg gccagggcgc
     3721 cgctgcgcga ccgcatgctg cctttcgtgg ggccggtggt gctgttcatc atctgggacc
     3781 tggtggtgcg cttcggcctg atcaaggcga tcctgctgcc cagccccctc gacaccttga
     3841 tcgcgctggc gcgcgggctg gccggcggcg acctgctcac cgatttcctg gtgacggtcg
     3901 gccgcacgct cgagtccttc gtcatcgcgg ccgtggtggg catgcccctg ggcgtgctgc
     3961 tgggcagcaa cgagcgcgcc taccgcagcg tggagttcct catcgatttt ttccgctcca
     4021 cgccttcatc ggccctgatc ccgctgtttc ttttgatctt cggcgtgtcc gacatcaaca
     4081 aggtggcgat cgcggccttc ggcgccctgc tcatcgtggt gttcaacagc gcctacggcg
     4141 tgatcaacgc gcgcaagcag cgggtcatgg cggccaaggt catgggcgcc tcgcgctggc
     4201 gcatcttcaa ggacgtgctc gtctgggaaa gcctgcagcc cagtttcgtg ggcctgcgct
     4261 cggcggtgtc gatggcgctg gtgatcgtga tcgtggccga gatgttcatc ggctcggaga
     4321 acggcctggg ccacgccatc atcgacgcgc agcaggtcct caacgtaaag accatgtacg
     4381 cggccatcct ggcggcgggc gcgctgggct acgtgctcaa tatcctgttc ctcatgctgg
     4441 agcggcgcgt ggttcactgg agcggaagat aatgaccacg gtattgaacg tccccgccta
     4501 cgccgacgtg ccggcgccgc gtttcgtgcc cggcccgacc ggcacgcaca tcactatccg
     4561 gggcctgacc aagtatttcg ccggctggcc gctgtacgag aacttcgatc tcgatattcc
     4621 caagaaccgc atcgtctcgg tgttcgggcc caatggctgc ggcaagagca cgctcatcaa
     4681 catgatcgcc ggcctggtgc ccatcgatca cggcgagatc ctcttcgacg gcaaatcgct
     4741 gaaggacacg cgcatcggct acgttttcca gaactaccgc gaggcgctgt ttccctggat
     4801 gcgcaccatc gacaatatcg cctatccgct cctgctcgaa ggcaagagca agacggaggt
     4861 cgaccgccgc atgcaggagc tggtggcctc gttcgacgtg aagttcgatc tcaaacgcta
     4921 tccctatgag ctttccggcg gccagcagca gacggcctcc atcatgcgcg ccctggcgcc
     4981 gcggcccgag gtgctgtttc tcgacgaacc cttctcggcg ctggatttcg agatgacgct
     5041 gttcatcagg gaaaagctgc aggaggtctt catgcagacc ggcaccacca tgatgctggt
     5101 ctcgcacgat ctcgaagagg ccgtgtacct ggccgaccag gtgctgctgc tcaccaagcg
     5161 gcccacgcgc gtggccgaga tactgaatta cggcgatgcg cggccgcgca cggtcgagac
     5221 cctgtcgcag cccggcttca tcgagaccaa gaagctcagt ctggagatat tccaacgcga
     5281 ggtgcgccga tgagcgcgga caccgtactg catttcgtgc gcgccagcgc aaccttgctg
     5341 ggactgccgc tggacgatgc gcaggccggc cgggtcgccg cgcacatggc ccgcacggcc
     5401 gggatggcgc ggctgctgga agccgcgccg ctggccgagg acgacgagcc ggccgaggtc
     5461 taccggcccg cgccgtttcc cgaagcgccc ggggcctagc cgtttgccgg cgccgcccgc
     5521 ttgccgttcg agacgatcac gatgaatttt cccgagattt ccccgggcca tggcctggac
     5581 agattgaacg cggtcgcgcg cggcgagatg acggcgcgcg cctggctgga agactgcgtc
     5641 gcccgcgtcg aaaccaccga cggccgggtc aacgcctata cgctgcgcac gttcgagcgc
     5701 gcccggcgcg aagccgacgc cgtcgacgcg ctacgggccg cgggccaacc gctgccgccc
     5761 ctggccggcc tgccctatgc ggtgaagaac ctgttcgaca tcgagggcga agtcacgctg
     5821 gcgggctcca aggtcaaccg cagccacgcg ccggccgccg ccgacgcggt gctggtgcag
     5881 cgcctgcggg cggccggcgc cgtgctggtc ggcgcgctca atatggacga atacgcctac
     5941 ggctttacca ccgagaacac gcactacggc cccacctgca acccgcacga cccggcgcgc
     6001 atcgcgggcg gctcctcggg cggctcaggc gccaccgtgg cggccgggca ggtgccggcg
     6061 gcgctggggt cggacaccaa cggttcgata cgcgtgcctg cctcgctgtg cggcgtctgg
     6121 gggctgaagc cgaccttcgg ccggttgtcg cggcgcggca gctatccctt cgtgcacagc
     6181 atcgatcatc tcggcccctt tgccgactcg gccggcatgc tggcgcgcgt ctacgacgcc
     6241 ttgcagtttc ccgatgcccg ggacccgggc tgccacgcgc gc
//
LOCUS       DASWPO010000332        22999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1597097, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000332 DASWPO010000000
VERSION     DASWPO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 22999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22999
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597097"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VGC69_17530"
     CDS             complement(<1..655)
                     /locus_tag="VGC69_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297135.1"
                     /translation="MSYLVLNGLGKRYGDMVAVDKLSLSVAQGEFISLLGPSGCGKTT
                     TLQMIAGFLEPTSGNIMLAGRSLNRTPANKRGMGLVFQNYALFPHMTAAQNVAFGLEM
                     QGVGKQERAARVGDALAMVGLAHLADRYPARMSGGQQQRVALARALVIKPSVLLLDEP
                     LSNLDAKLREEMQLELRAIQRTVGTTTILVTHDQSEALALSDRVVVMNQGRVEQVADP
                     "
     gene            complement(705..1736)
                     /locus_tag="VGC69_17535"
     CDS             complement(705..1736)
                     /locus_tag="VGC69_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297136.1"
                     /translation="MNKVFAVLTAAVLAAGCAMDASAQQKLVVAGYGGSFEDAMRKDI
                     LPAFVQAHNATFDYVAGNSTDTIARLLAQKSNQQIDVAIVDDGPMYQAIALGLCAPIQ
                     SLPKADLYPSALFPEDKGVGLGLNATGIMYNKKVFADKGWAAPTSWNDLKDPKYKQLL
                     VIPPMNNTYGLHSLIEYAKLGGGGEKNIDPGFKTFKDAVGPNVLVYEPSPGKMTELFT
                     SGQTVIGVWGSSRVKALADTGFPVGFVYPKEGAYALMVAGCPVVKSSPNPLAQALLTY
                     LASPEGQAKMAVALGAAPVNKKVVAKDDPNVPLPIGQRAADLIVVDWDTVNQQRDDWN
                     KRWTREIER"
     gene            1886..2680
                     /locus_tag="VGC69_17540"
     CDS             1886..2680
                     /locus_tag="VGC69_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0297137.1"
                     /translation="MKSTTTPAPDMIQASAAPEAGQDRAPRYKAIYQDLAQAIRAGRY
                     PVMSLLPTELELCAQYQASRHTVREAIRMLTETGMVSRRAGVGTRIEAAKPPTRYTQR
                     ISQLADLFLYIQNADLRLRDVRAIKANARQAEMLGCPLGQQWLHVRAVKLLGGKSEPV
                     AYSEAFVHPDYAAIRDDIGKVRMPLSALIESRYSQRIREVGQDFSATPIPKAMAEQLK
                     VAPKTAGLVITRRYYGEHQIPMLVTATTFPHDKMKYSMSLQVDWPD"
     gene            complement(2685..4406)
                     /locus_tag="VGC69_17545"
     CDS             complement(2685..4406)
                     /locus_tag="VGC69_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0297138.1"
                     /translation="MAEPTIERSADALTDPIGMEVFCNRLLSITEDMNNTLVRASFST
                     NIKERKDCSVALFDAAGRLVAQGTQIPLHLGSLNGAMQAVLAAHEIESIADGDVFICN
                     DPYLANGSHLPDINIVTPVFWEGALRFFAANIAHHSDVGGPVPGSIGGNLGSIFAEGI
                     RIPVCRIARAGVVDEDLLNLICVNTRDPEERQLDLRVQMATNRRGAAAMQGLIRQMGL
                     QDVLRSVDDVIAYTRRRLLNRIAELRQGSYTFRNELDDDGLGGGEPVPIQVTLTVGAD
                     GLHFDFTGSGKQARGAMNLPVNALRASVYYAVKALLDPDIAPNAGLFEPITIHAPPGT
                     ITNPEHPAAVGARSITAQKVAGAIFGAFRGLLPPEKIMASGNDCCPAIVFSGNWSTRP
                     GQFVYLETLGGGSGARLDVDGMDAVHVHMTNTSNLPVEALENEYPLIMDEYAMIQDSG
                     GAGRTRGGMGIAKQIRSLVPGIVFSARSDSHTVGVASGVDGGADGRRARLLRNHGTDR
                     EQVLFSKTSNITLEAGESVRIETSGGGGYGDWHERPADLVERDLLDGKISAQQARERY
                     GYRGPLA"
     gene            complement(4399..6465)
                     /locus_tag="VGC69_17550"
     CDS             complement(4399..6465)
                     /locus_tag="VGC69_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0297139.1"
                     /translation="MYRIGIDVGGTFTDFTMIDEGTGQVHFHKVPSTPHDPSEAIAQG
                     IAGLLADQGVAPAQVSHVGHGTTVATNLIIERKGARVGLITTRGFRDILEIGRQTRPH
                     LYDYGVGKPPVAVPRQFRIEVAERIDASGAVHVPLDEAAVREAARYFAQHGIGAVTIC
                     FLHAYRNPAHEQRAREIVQQALPGIYVSLSSEVLPEFREYERLSTTVLNAAVGPRMAG
                     YLERFLERVRELGIDHEPHTVHSNGGLMSIASVRQFPVRTCLSGPAAGVVGAAAVGRA
                     IGSPDLVTFDVGGTSTDVSLICDGRPMFTSHRQVAGYPVRTPMVDIHVIGAGGGSIAW
                     MDDAGALKVGPHSAGAVPGPVGYGRGGAQPTITDAEITLQRLNPVALLNGRMPVYAEA
                     ARAVIGEQVAKPLGLSVEDAAEGILRIAAANMSRAIRAVSTERGYDLSRFALFAYGGA
                     GPLHAVEVAEECGIPRVIVPQEPGTMCARGILLSDISFDFVRSQITLATPQNWPGVAQ
                     RFETLRAQATSWLEQEGVAPALRAINFAIDARYEGQNFEVQVPLAGIGASGAEGVEAF
                     LAAFAEAHEREYGYVVDGRAVQIINCRAQAVGQVIKAPLARRTVAGTVEGARIGARRA
                     YFGATHGWVETPVYDRNQLPTGATLAGPALVEEMSSTTVVGVGHRAQVDDYGNLIILL
                     QGAEHG"
     gene            complement(6508..7503)
                     /locus_tag="VGC69_17555"
     CDS             complement(6508..7503)
                     /locus_tag="VGC69_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297140.1"
                     /translation="MAATRLRRIGRAALAGMALAVGSTVCNTAWAAWPHDKTIRLVVP
                     FAAGGSTDLIARKVAEGLSKDLDANVIVENRPGAGGTIGSDFVARQPADGYTILMGSV
                     STHGSAECIYAKLPYDPIKDFTPLGVVASIPNLMVIGQSVPANTLQEFIALLKKDPEH
                     YSYASNGLGTSNHLAAELFKATAGVSMTHVPYRGSGPALIDVLGGQVNMMMDVVMTSY
                     PYVKDGKLKALAVTSSQRHPMLPDVPTVAEQGFPGFEAIVWFGLLAPAKLPEAVSKPF
                     IASFERVMHSPDMKTYLEGQGAEIPDITGPAFATMIHDEVDKWCKVAKQANIHLD"
     gene            7676..8587
                     /locus_tag="VGC69_17560"
     CDS             7676..8587
                     /locus_tag="VGC69_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0297141.1"
                     /translation="MKNPDLNLLLHFDALMSCRSLTRAAEQLGVSQPAVSAALHRLRR
                     LFNDPLLVREHGVWLPTEQAADLQRRFAPLLAQWRRSTRGPDAFDPAHSSRTLSLYAT
                     DYVQYTLLPRVLPDLARDAPHFQLRIVPARPRHGLSMLDTNHVELIAGYFPDPTPDLR
                     ARLLYDEPAVCVVRENHPCLRKRWNLDAYLKYGHLDLAAHTRFFGNVIDRVLQGQNRQ
                     RTIAATLSSYLVCPFVIGSSDLIATMPTSVARAMQAATRTEIVKVPFALPNIAVSLYW
                     HERYHNDPGHAWLRQYIAERVSGTSGP"
     gene            complement(8622..9059)
                     /locus_tag="VGC69_17565"
     CDS             complement(8622..9059)
                     /locus_tag="VGC69_17565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0297142.1"
                     /translation="MQDIEQRIAASFGAQGLMETLGARLALVEEGEVHVALPFSRHIA
                     QQHGFLHAGAVTSIVDNACGYAALTKAPPGCEVVTVEFKVNFLRPALGRHFLAVGKVQ
                     NAGRQLAVCTGEVRAFAGDDASYKVVALMQATIANVRNEVTGR"
     gene            complement(9102..10025)
                     /locus_tag="VGC69_17570"
     CDS             complement(9102..10025)
                     /locus_tag="VGC69_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0297143.1"
                     /translation="MGDPVHIPATDGYALRGFVWRRAGSPPRPVVIVNSATSVRCRYY
                     ERFADALHEQGCDVLTYDYRGIGESRHGSLRALSAGWLDWGERDFEGVLRYVAANFPG
                     QPIRVVGHSIGGFVIGLAASSHLIDRIFTMGAQFAYWRDYAPDSRSRMFLRWHIVMPL
                     IVRLFGYVPAKRLGWMEDTPKGVALDWARMGPRFESSLRRDGQAMAGRFGQRFGQVRA
                     PMLALGIDDDPFGTVPAIDRLLGYYTHSERHHLRLAPSAVEQDAIGHFAFFHKRFRES
                     LWPLASDWLLRQRLPEPLPGTLMRYPAADML"
     gene            complement(10047..11210)
                     /locus_tag="VGC69_17575"
     CDS             complement(10047..11210)
                     /locus_tag="VGC69_17575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY0297144.1"
                     /translation="MSQNLSTVTTIEELRIIAQKRVPRMFYDYADSGAWTESTYRANE
                     SEFQKIKFRQRVAVDMDGRSLRTTMAGHDVAMPVALAPTGLTGMQHADGEMLAAQAAA
                     SFGVPFTLSTMSICSIEDVAAATKQPFWFQLYVMRDREFVMNLIDRAKAANCSALVLT
                     LDLQILGQRHKDIKNGLSAPPKPTLRNLINLATKPQWCLGMLGTKRRTFGNIVGHAKS
                     VTDLSSLSSWTAEQFDPRLSWDDVAWIKERWGGKLILKGILDAEDARLAADSGADALI
                     VSNHGGRQLDGARSTIEVLPSVVEAAGSKIEVWLDSGIRSGQDVLKAVALGAKGTMIG
                     RSFLYGLGAYGRAGVTHALEIIRKEMDVTMALCGRKNIADVDRSILVPGTYPE"
     gene            11312..11596
                     /locus_tag="VGC69_17580"
     CDS             11312..11596
                     /locus_tag="VGC69_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY0297145.1"
                     /translation="MSTPHLETLLVTVHGKVQGVGYRHATVRHAHLLGVTGWVQNLEN
                     GTVEALVQGTPDQVDRMLEWLRRGPPAAVVSELETERQFIDKRYGRFEQV"
     gene            complement(11627..13039)
                     /locus_tag="VGC69_17585"
     CDS             complement(11627..13039)
                     /locus_tag="VGC69_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0297146.1"
                     /translation="MDWKSICDMTALQMRVEIETGHLSARQAVEAHLASIEARNPAVN
                     AIVTLDAEGALRQADAADAAQAASRPLGLLHGLPVAHKDSFLTAGMRTTYGSPVFRDF
                     VPARTSLPVTRQREAGAITLGKTNLPEFGAGSHTFNPVFGATRNPYDPGRTPGGSSGG
                     AAAALAARMLPLADGTDMGGSLRNPASFCNVVGLRPSPGRVPQWPSATPYSTLTVAGP
                     MARTVADAALMLAALAGPDARDPYSIEQDPAKFAGSLERGFAGTRIACALSWGGLPVE
                     PAVLRALQAQLPVFEQLGCKVEEACPDFGDADGAFHALRAQNFAVSQGETLRKHRDKL
                     KQTVVWNTELGLSQPATALIHAERERAALFERMHAFMGQYEFMVGVVSQVAPFPVATE
                     YPTEIDGAPMRNYIDWMRSSYYLTMTGHPAISVPCGFTAEGLPVGIQIVGRYRDEWGV
                     LQLAHAFEQATQFWKRAPSL"
     gene            13083..14030
                     /locus_tag="VGC69_17590"
     CDS             13083..14030
                     /locus_tag="VGC69_17590"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0297147.1"
                     /translation="MAYSKHSLARRLKLQQLAIFESVLACGSMLAASRELHMTQPALS
                     KAIQELEEHFGQPLLIRTRRGVQPTDFGLMLRNHSQSLMTELRSLADELNAWNSSETW
                     DAEVSGHVAVGSLLAASAHLLPQAVLRLRETTPNVSVQVRVGVNDKMFPELVRGELDV
                     MVGLMPDHADDPVFEHVKLYDETLCVVAGRQHPLATSQNIDPARFEAVDWILPTPDTE
                     AMHAVDALFAELGFRKPARIVESVSIMTNLGLLVESRMIAVMPFSVARKFVHLGVLSI
                     LPLGREIPFGPIGYTVPRGRPASPAVQRFLMALKDVAVE"
     gene            14077..14913
                     /locus_tag="VGC69_17595"
     CDS             14077..14913
                     /locus_tag="VGC69_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0297148.1"
                     /translation="MRLQILSDLHLEANPDFAFTPMPGVDVLVLAGDIGSYQEGSALD
                     GPDFGLERYTPLGRAADWPKVLFVPGNHEFDGLDFDATTQRLRQTCERLGIAWLEREV
                     AVIDGIRFVGTTLWTDFEALAARQHTETRRQQQREKAYRAANFYLNKNTTFLDGQPML
                     AEDLRQLGLDCQGWLRQALAQPYTGKTIVVTHFAPTLRSADPRYGMTPGTAGFCNGLD
                     DLLPYADLWIHGHLHCPNDYRVPGQRDGRAVSCRVVANPLGYADKGEQEGFKPDWVIE
                     LP"
     gene            15054..16535
                     /locus_tag="VGC69_17600"
     CDS             15054..16535
                     /locus_tag="VGC69_17600"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HEY0297149.1"
                     /translation="MATFDLPASLRPTRNYVGGEFVATDKTFQNISPIDGRLLAEVCE
                     ADAALVDRAVRAGRAALEGPWGAMSVDERARCLHKVADIIERRFDDFLAAEIADTGRP
                     LAQARKLDVYRGIANFRTFADLGRLRGSEFFETRLGDGTQLINYVTRKPLGVVAIIAP
                     WNLPILSLTWKAAPALICGNAVVCKPSEETPSSATLIAEAFHEAGVPPGVFNLVHGFG
                     PGSAGEALTTHPGIDAVAFTGESRTGAAIMRAVADGVKDISFELGGKNAAIVFADADF
                     DAAVQGIVASSFTNAGQVCLCSERVYVERPIFERFVAALKERTEALKVGWPDDEDVFM
                     GSLISRGHREKVLSYFDLAVQEGATVVAGGRAPVFHDARDGGAYVLPTIWTGLPDTAR
                     CMREEVFGPVCHIAPFDSEDEVARRANDSAYGLAASVWTTNLSRAHRFGPRLHAGIVW
                     INTWFSRDLRTPFGGGGLSGIGREGGEYSLNFFSDISNICIKV"
     gene            16538..16981
                     /locus_tag="VGC69_17605"
     CDS             16538..16981
                     /locus_tag="VGC69_17605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0297150.1"
                     /translation="MSDKSADQSAGVIAGKAVPRGKFPHYKRAGDFIFVSGTSSRRPD
                     NSFEGVQVDPLGVTNLDIRAQTRAVIHNIGEILAGAGAGLADLVEAQVFLVSMNDFGG
                     FNEVWAEFFDYTGPTRTTVAVHQLPHPHLLIEIKATAYSPLGTHP"
     gene            16997..17572
                     /locus_tag="VGC69_17610"
     CDS             16997..17572
                     /locus_tag="VGC69_17610"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEY0297151.1"
                     /translation="MSTLQYGYPFNFKRWLDDNAHLLKPPVGNQQIWKDSDFMVTVVG
                     GPNQRTDFHDDPLEEFFYQFKGNAHLLLWDRGRYERVHLKEGDMFLMPPHVSHSPQRP
                     EPDSRCLVIERVRPKGQPDALHWDCAHCGTVVKRYEFQLENIVVDLPPVYEQFYGTSE
                     AERACPQCGHVHPGRDYQTWHADLNARHPDL"
     gene            17616..18611
                     /locus_tag="VGC69_17615"
     CDS             17616..18611
                     /locus_tag="VGC69_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0297152.1"
                     /translation="MIDIHSHFFPEIPQALAREFDAERAVWLRVDEGQDTGQIMLGDK
                     PFRPVYRALWDAGYRVGELDAQGVAMQLTCATPVMFGYSWDAAKAAEWAARMNDLAVD
                     FCAAHPTRLKALSQVPMQDIKLATAEAKRAQLRGCVGVQIGNHVGDKDMDHPDMIDFL
                     TFCAEEGIPVFAHPWDMMGGPGRMKKWMLPWLVAMPAETQLGMLSLILSGALEHIPET
                     LKLCFAHGGGSFAYLLGRVDNAWQHRDIVRQDCPRPPSEYARRLWCDSALFDHKALDM
                     LVDAVGVDHVMMGSDYPFPLGEQPIGSLVSGSPYLDEAARKKILADNAKVFFNLA"
     gene            18719..19945
                     /locus_tag="VGC69_17620"
     CDS             18719..19945
                     /locus_tag="VGC69_17620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297153.1"
                     /translation="MEPQASTLSAAKRRTIYLLCFLAIIIDGFDTTTIAYVAPRLSAV
                     WQLARGEIAPAFIATSIGAVIGYLLSGWAAARLGKRKLIAWSIFVFGVLTIATAYVGT
                     LTELTAIRLFTAIALGFVVPAAISSAADHSDDRTRAAVTIFVTTGLSLGAAAGGFLAA
                     FLMVRFDWQSVFITGGVLAFCLVPFVWQSLATDGLESETAKTANGGLAALFKEGQASS
                     TILIWALAFVSFMVTYLFMFWVPTLLTLFKFSPSQAPLGSGAFGLGGVLGNLVILPFV
                     KRYGPERLVIATSLLCIVCLYALAIGALQPMLVLVAIAGVGAGLVSACVSQSTLSVLR
                     FRADLRTSAVGAAAAVGRIGSIVGPAIGGMLVASKLSPEMIMVVSSVPVVGIILILIG
                     LLAVARPALPLSRQAG"
     gene            19975..21444
                     /locus_tag="VGC69_17625"
     CDS             19975..21444
                     /locus_tag="VGC69_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY0297154.1"
                     /translation="MQSKNPTVDTPAGIIHGAAVGRVHAFQGIRYGANAGRFKAVVPI
                     AQSGASPQVPPCAFPQNKNRLEFVMGDAIHGLPQAEDAFLLDLWVPENHAGKLPVLVF
                     IHGGAFISGGGSGTRYDGAMFAQEQSAIVVTLNYRLGALGHLAIDRVSDGNLPFDDIL
                     CALGWIRDNIARFGGDPANVTVAGQSAGAWYAYLLGVSPAAQGLLRRNVLFSLPFFPP
                     MAQAAAAELAAAFGEMLGGADPSEAAVEDLLAVQGKVMRGTARFGDLTLGFYPVASDG
                     KVPASLFDYAKSAADAHIEGTLAGYTREENAAFMIQDPRTRNPDPAAVDQWFRGHFGA
                     MGGIKRSYLDSLRPAPTPYTSLVDGGTHISFASPTVQVAASFGRRGLNSWLYRFDLQS
                     GTPDLHSPHCMELPFLFANRRQWTDAAMLAGIDDRAFDRAGRCFRHAIGSFARGDAPG
                     FTPADPWAQFREGAWTCHLFGDTDHAGTISLEPSAFIEN"
     gene            complement(21456..>22999)
                     /locus_tag="VGC69_17630"
     CDS             complement(21456..>22999)
                     /locus_tag="VGC69_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297155.1"
                     /translation="DWTQKDSTIWPVQAFAWRDLTARRGASYLYKITPVSGDPAAGLP
                     LTLDEAGSLMAGPVTLTPKRGKFSTYFNRGILSTQWLAHQLPETANGTPDSAVLVKAI
                     ATPGDRIRTALAGDIIAGVTSLLDRAAHQGGACYLALYELQDEELIDRLMAVRNLHLI
                     LSNTGSKDSENAPARERLHGRKGAQVIDRFVPSGHIGHNKFCVYVDATKVPQAVLLGS
                     TNWTNTGLCGQSNNALVVEDEVLASAYFDYWGRLRDDSLEDDSRQGAALRSSNATPGA
                     KDVPVDKGKATVWFSPNTPHARASGQRKKNEATPPDLADLFTLMAQAQQAILFLEFEP
                     GTPSVIDQAAACLDARPGLFVRGATTDRKSPGAFATQLTHRGGEGDDVVSAMAITSPH
                     ADWEKELLKSSDGAHAIIHDKIVVIDPMSERCVVATGSHNQGFRASYNNDENLMIVQG
                     HQPLALAYAAHVLDVYSHYRFRYNVSRSSSKAFSALSPDDSWQKKYFDPANPASKDAG
                     VWFPG"
BASE COUNT         3657 a         8047 c         7501 g         3794 t
ORIGIN      
        1 acgggtcggc cacctgctcg acgcggccct ggttcatgac cacgacgcgg tccgacaggg
       61 ccagggcctc ggactggtcg tgcgtgacca ggatggtggt ggtgcccacg gtgcgctgga
      121 tggcgcgcaa ttcgagctgc atttcctcgc gcagcttggc gtcgaggttg gacagcggct
      181 cgtcgagcag cagcaccgag ggcttgatca ccagcgcccg ggccagcgcc acgcgctgct
      241 gctggccgcc cgacatgcgc gccggatagc ggtcggccag gtgcgccagc cccaccatcg
      301 ccagcgcatc gcccacccgg gcggcgcgct cctgcttgcc cacgccctgc atttccaggc
      361 cgaaggccac gttctgcgcg gccgtcatgt gcgggaacag ggcatagttc tggaacacca
      421 gccccatgcc ccgcttgttg gccggcgtgc ggttgaggct gcggccggcc agcatgatgt
      481 tgcccgaggt cggttcgagg aagccggcga tcatctgcag ggtggtggtc ttgccgcacc
      541 ccgaaggccc cagcagcgag atgaactcgc cctgcgccac cgacaggctc agcttgtcca
      601 cggcgaccat gtcgccgtag cgcttgccca gcccgttcaa gacgagatag ctcatgaaag
      661 acttctgcgg ccgcggcgtg gatgaaaggc tgaaaggcgg ggcatcaacg ctcgatctcg
      721 cgcgtccagc gcttgttcca gtcgtcgcgc tgctggttga cggtgtccca gtcgaccacg
      781 atcaggtcgg cggcgcgctg gccgatgggc aggggcacgt tgggatcgtc cttggccacg
      841 accttcttgt tgaccggcgc ggcgcccagg gcgacggcca tcttggcctg gccctcgggc
      901 gaggccaggt aggtgagcag cgcctgcgcc agcgggttgg ggctggactt gacgaccggg
      961 cagcccgcca ccatcagcgc gtaggcgcct tccttcgggt agacgaagcc caccgggaag
     1021 ccggtgtcgg ccagcgcctt gacgcggctg ctgccccaca cgccgatgac cgtctggccg
     1081 ctggtgaaca gctcggtcat cttgcccggc gaaggctcgt agaccagcac gttggggccg
     1141 accgcgtcct tgaaggtctt gaagccgggg tcgatgttct tctcgccgcc gccgcccagc
     1201 ttggcgtact cgatcagcga gtgcaggccg taggtgttgt tcatcggcgg gatgaccagc
     1261 agctgcttgt acttggggtc cttcaggtcg ttccacgagg tgggcgccgc ccagcccttg
     1321 tcggcgaaga ccttcttgtt gtacatgatg ccggtggcgt tcaggcccag gcccacgccc
     1381 ttgtcttccg ggaagagcgc gctggggtac aggtcggcct tgggcaggct ctggatgggc
     1441 gcgcacaggc ccagggcgat ggcctggtac atggggccgt cgtcgacgat ggccacgtcg
     1501 atctgctggt tgctcttctg cgccagcagg cgggcgatcg tgtcggtgga gttgccggcc
     1561 acgtagtcga aggtggcgtt gtgcgcctgg acgaaggccg gcaggatgtc cttgcgcatg
     1621 gcgtcctcga acgaaccgcc gtagcccgcg accaccagtt tctgctgcgc gcttgcatcc
     1681 atggcgcagc cggcggccag gacggccgcc gtgagcactg caaagacctt gttcatgatc
     1741 cccccgattt cggttcagcg atgtttgccg gtatatccgg ccttgagatt tgaccgtaca
     1801 atttgtccgg acatgaatgt ccggacaaat aatcctgcca gcgcggcaat cttgtcaata
     1861 cgaggttgtc ctgaaaccac ccgcgatgaa atccaccacg acgcccgccc ccgacatgat
     1921 ccaggcctcc gccgcgccgg aggccgggca agaccgcgcg ccccgctaca aggccatcta
     1981 ccaggatctc gcccaggcca tccgcgccgg gcgctatccg gtgatgtccc tgctgcccac
     2041 cgagctcgag ctgtgcgcgc agtaccaagc cagccgccac acggtgcgcg aagccatccg
     2101 catgctgacc gagaccggca tggtgtcgcg ccgcgccggc gtgggcacgc gcatcgaggc
     2161 cgcgaagccg ccgacacgct atacgcagcg catctcgcag ctcgccgacc tttttctcta
     2221 catccagaat gccgacctgc ggctgcgcga cgtgcgcgcc atcaaggcca atgcgcggca
     2281 ggccgagatg ctgggctgcc cgctcgggca gcaatggctg cacgtgcgcg ccgtcaagct
     2341 gctgggcggc aaatccgagc cggtggccta ttccgaagcc ttcgtgcacc ccgactacgc
     2401 cgccatccgc gacgatatcg gcaaggtgcg catgcccctg tcggccctga tcgaatcccg
     2461 ctacagccag cgcatccgcg aagtcggcca ggacttctcg gccacgccca tccccaaggc
     2521 catggccgaa cagctcaagg tcgcccccaa gactgccggc ctggtcatca cccggcgcta
     2581 ttacggcgaa caccagatcc ccatgctggt cacggccacc accttcccgc acgacaagat
     2641 gaaatactcg atgtcgctgc aagtggactg gcccgattga tcccttaggc cagcgggccg
     2701 cggtagccgt agcgctcgcg cgcctgctgc gcgctgatct tgccgtcgag caggtcgcgc
     2761 tcgaccaggt cggccggccg ttcatgccag tcgccgtagc cgccgccgcc cgaggtctcg
     2821 atgcgcacgc tctcgccggc ctcgagcgtg atgttcgagg tcttcgaaaa cagcacctgc
     2881 tcgcggtcgg tgccgtggtt gcgcaggagc cgggcgcgcc ggccgtcggc gccgccgtcc
     2941 accccgctgg ccacccccac ggtgtggctg tcggaacgcg ccgagaacac gatgcccggc
     3001 accagcgaac ggatctgctt ggcgatgccc atgccgccgc gcgtgcggcc ggccccgccg
     3061 gaatcctgga tcatggcgta ttcgtccatg atcaggggat attcgttttc cagcgcctcg
     3121 accggcaggt tggacgtgtt ggtcatgtgc acatgcacgg cgtccatgcc gtcgacgtcc
     3181 aagcgcgcgc cgctgcctcc gcccagcgtt tccaggtaga cgaactgccc gggccgcgtc
     3241 gaccagttgc cggagaacac gatggccggg cagcaatcgt tgcccgaggc catgatcttc
     3301 tccggcggca gcaggccgcg gaaggcgccg aagatggcgc ccgccacttt ttgcgccgtg
     3361 atcgagcgcg cgcccacggc ggccgggtgc tcgggattgg tgatggtgcc cggcggcgca
     3421 tggatggtga tgggctcgaa caagcccgcg ttcggcgcga tgtccgggtc cagcagggcc
     3481 ttgacggcgt agtagaccga ggcgcgcagc gcattgacgg gcaggttcat ggcgccgcgc
     3541 gcctgcttgc ccgacccggt gaaatcgaag tgcaggccgt cggcgccgac ggtgagcgtg
     3601 acctggatgg gcacgggctc gccgccgccc aggccgtcgt cgtcgagctc gttgcggaag
     3661 gtgtagctgc cctgcctgag ctcggcgatg cggttgagca gccggcgccg cgtataggcg
     3721 atcacgtcgt ccaccgagcg cagcacgtct tgcaggccca tctggcggat caggccctgc
     3781 atggccgcgg cgccgcgccg gttggtcgcc atctgcaccc gcaggtcgag ctggcgctct
     3841 tccgggtcgc gcgtgttgac gcagatgaga ttgagcaggt cttcgtccac cacgccggcg
     3901 cgcgcgatgc ggcacaccgg aatgcgtatg ccttcggcga agatcgaccc caggttgccg
     3961 ccgatcgagc ccggcaccgg cccgcccacg tccgaatggt gcgcgatatt ggccgcgaag
     4021 aaccgcagcg cgccctccca gaacaccggc gtgacgatat tgatgtcggg caggtggctg
     4081 ccgttggcca ggtagggatc gttgcagatg aacacgtcgc cgtcggcgat ggactcgatc
     4141 tcgtgcgccg ccaggacggc ctgcatggcg ccgttgagcg agcccaggtg cagcgggatc
     4201 tgcgtgccct gcgccaccag ccggccggcc gcgtcgaaca gggccaccga gcaatccttg
     4261 cgctctttga tgttggtgga gaacgaagcc cgcaccaggg tgttgttcat gtcttccgtg
     4321 atcgacagca gccggttgca gaagacctcc atgccgatcg ggtcggtcag tgcgtcggcg
     4381 gatcgttcga tggtgggttc agccatgctc ggctccttgc agcagaatga tcaggttgcc
     4441 gtagtcgtcg acctgggcgc ggtggcccac gccgaccacg gtggtcgagc tcatttcctc
     4501 gaccagggcg gggccggcca gcgttgcgcc ggtgggcagc tggttgcggt cgtacacggg
     4561 cgtctcgacc cagccgtgcg tcgcgccgaa atacgcgcgc cgcgcgccga tgcgcgcgcc
     4621 ttctaccgtg cccgccaccg tgcgccgggc cagcggcgcc ttgatcacct ggcccacggc
     4681 ctgggcgcgg cagttgatga tctgcaccgc gcgcccgtcg accacatagc cgtattcgcg
     4741 ctcgtgggcc tcggcgaagg ccgcgaggaa agcctcgacc ccttccgcgc cgctcgcgcc
     4801 gatgccggcc agcggcacct gcacctcgaa gttctggccc tcgtagcggg cgtcgatcgc
     4861 gaagttgatc gcacgcaagg cgggcgcgac gccttcctgc tccagccagg acgtggcctg
     4921 cgcgcgcaag gtctcgaagc gctgcgccac gcccggccag ttctgcggcg tggccagggt
     4981 gatctggctg cgcacgaagt cgaaggaaat atcggacagc aggatgccgc gcgcgcacat
     5041 cgtgcccggc tcctgcggca cgatcacgcg gggaatgccg cactcctcgg ccacctccac
     5101 cgcgtgcaag gggccggcgc cgccgtaggc gaacagggcg aacctggaga ggtcgtagcc
     5161 ccgctcggtg gacacggcgc ggatggcccg gctcatattg gccgcggcga tgcgcaatat
     5221 gccttcggcc gcgtcttcca cgctcaggcc cagcggcttg gccacctgct cgccgatcac
     5281 ggcgcgcgcc gcctcggcgt agacgggcat gcggccgttg agcagggcca cgggattgag
     5341 ccgctgcagg gtgatctcgg catcggtgat ggtgggctgc gcgccgccgc ggccgtagcc
     5401 caccgggccc ggcacggcgc cggcgctgtg cgggccgacc ttcagcgcgc ccgcgtcgtc
     5461 catccaggcg atgctgccgc cgcccgcgcc gatcacgtgg atgtcgacca tgggcgtgcg
     5521 caccggatag cccgccacct ggcgatgcga ggtgaacatg ggccggccgt cgcagatcag
     5581 cgacacgtcg gtgctggtgc cgcccacatc gaacgtgacc aggtcgggac tgccgatcgc
     5641 gcgccccacg gccgccgcgc ccaccacgcc ggccgcagga cctgacaggc aggtgcgcac
     5701 cgggaactgg cgcacgctgg cgatcgacat cagcccgccg ttcgaatgca cggtgtgtgg
     5761 ctcgtgatcg atgcccagct cgcgcacccg ctccaggaag cgctccaggt agccggccat
     5821 gcgcgggccg accgcggcat tgagcaccgt ggtcgacagc cgctcgtact cgcggaactc
     5881 gggcagcact tccgacgaca ggctgacgta gatgcccggc aacgcctgct ggacgatctc
     5941 gcgggcccgc tgctcgtgcg ccggattgcg gtaggcgtgc aggaagcaga tggtcaccgc
     6001 gccgatgccg tgctgcgcaa aataccgggc cgcctcgcgc acggccgcct cgtccagcgg
     6061 cacgtgcacc gcccccgacg catcgatgcg ttcggccacc tcgatgcgga actgccgcgg
     6121 caccgccacc ggcggcttgc ccacgccata gtcgtacagg tgcggccgcg tctgccggcc
     6181 gatctcgaga atgtcgcgaa agccccgggt ggtgatcagg cccacgcggg cgcccttgcg
     6241 ctcgatgatg agattggtgg cgaccgtcgt gccgtgcccc acgtgcgaga cctgcgccgg
     6301 cgccacgccc tggtcggcca gcaggccggc gatcccctgg gcgatcgcct ccgacggatc
     6361 gtgcggcgtg gacggaacct tgtggaagtg cacctgcccg gtgccttcgt cgatcatcgt
     6421 gaagtcggtg aacgtgccgc cgacgtctat ccctatgcgg tacatgcgtg tgctgccttg
     6481 aagcgtctga acgaaatgga ggaggggtca atccagatgg atattggcct gcttggccac
     6541 cttgcaccac ttgtccactt cgtcatggat catggtggcg aaggccggcc ccgtgatgtc
     6601 gggaatttcg gcgccctggc cttccaggta ggttttcatg tcggggctgt gcatgacgcg
     6661 ctcgaagctg gcgatgaacg gcttgctcac ggcctccggc agcttggccg gcgccagcag
     6721 gccgaaccac acgatggcct cgaaccccgg gaagccctgc tcggccacgg tgggcacgtc
     6781 gggcagcatg ggatgccgct ggctggacgt gacggccagc gccttgagct tgccgtcctt
     6841 cacatagggg taggacgtca tgaccacgtc catcatcatg ttcacctgcc cgcccagcac
     6901 gtcgatcagc gccggaccgg acccgcggta gggcacgtgc gtcatggaca cgccggccgt
     6961 ggccttgaac agctcggccg ccaggtggtt ggacgtgccc agcccgttgg acgcatagga
     7021 ataatgctcg ggatctttct tgagcagggc gatgaattcc tgcagggtgt tggccggcac
     7081 gctctggccg atcaccatca ggttgggaat ggaggccacc acgcccagcg gggtgaagtc
     7141 cttgatcggg tcgtagggca gcttggcata gatgcattcc gcgctgccgt gcgtgctcac
     7201 cgaacccatc aggatggtgt agccgtcggc cggctggcgc gccacgaagt ccgagccgat
     7261 ggtgccgccc gctcccggcc ggttctcgac gatgacattg gcgtcgaggt ccttgctcag
     7321 gccctcggcc actttgcggg cgataaggtc ggtggacccg cccgccgcga acggcaccac
     7381 caggcggatg gtcttgtcat gcggccaggc ggcccatgcg gtgttgcaaa cggtactgcc
     7441 gacggccagc gccatgccgg ccaatgcggc acggccgatg cggcgcaaac gagttgctgc
     7501 catgtacttc cccttgtatt ggtcgaatcc attcccgggg cggatgccat gcgtcgcggc
     7561 cggctccgcc catcgccgtt caactgcaaa acagcctcat gctaggcgca tggcggttct
     7621 agcagcagcg gttaacgctt atattggtat caatctcatt gatgacgccc gcccagtgaa
     7681 gaatcccgac ctcaacctgc tgctgcattt cgacgcactc atgagctgcc gcagcctgac
     7741 gcgggcggcc gaacagctgg gcgtgagcca gcccgccgtc agcgcggccc tgcaccgcct
     7801 gcgccgcctg ttcaacgacc ccctgctggt ccgcgagcac ggcgtctggc tgcccaccga
     7861 gcaggccgcc gacctgcagc gccgctttgc cccgctgctc gcgcaatggc gccgcagcac
     7921 gcgcggcccc gacgccttcg acccggcgca ctcctcgcgc acgctgtcgc tctacgccac
     7981 cgattatgtg cagtacacgc tgctgccgcg cgtgctgccg gacctggccc gcgacgcccc
     8041 gcacttccag ctgcgcatcg tgccggcccg gccgcgccac gggctcagca tgctggacac
     8101 caaccacgtc gagctgatcg ccggctactt tcccgacccc acgcccgacc tgcgcgcgcg
     8161 cctgctctac gacgaaccgg ccgtctgcgt ggtgcgcgaa aaccacccct gcctgcgcaa
     8221 gcgctggaac ctcgacgcct atctcaaata cggccacctg gacctggcgg cccacacccg
     8281 cttcttcggc aacgtcatcg accgcgtgct gcagggccag aaccgccagc gcaccatcgc
     8341 cgccacgctc tcgagctacc tggtctgccc cttcgtcatc ggcagctcgg acctcatcgc
     8401 caccatgccg accagcgtcg cgcgggccat gcaggccgcc acgcgcaccg agatcgtcaa
     8461 ggtgcccttc gccctgccga acatcgccgt ctcgctctac tggcacgagc gctaccacaa
     8521 cgacccgggc catgcctggc tgcgccagta catcgccgag cgcgtgtccg gcacatccgg
     8581 cccgtgaatg ccggaacggc cgaatcaggc aaccggacat atcaccggcc ggtcacctcg
     8641 ttgcggacgt tggcgatggt ggcttgcatg agggcgacca ccttgtagga agcgtcgtcg
     8701 ccggcaaacg cccggacttc gccggtgcag accgcgagct gcctgcccgc gttctgcact
     8761 tttccgacgg ccaggaaatg cctgcccaat gccggccgca ggaaattgac cttgaactcg
     8821 acggtgacga cttcacagcc tggcggcgcc ttcgtcagcg ccgcatagcc gcaggcgtta
     8881 tcgacgatgc tggtgaccgc gccggcgtgc aaaaagccat gctgctgggc aatatgcctg
     8941 gagaacggca gcgcgacatg cacttcaccc tcttccacca gggcaagccg cgcgcccagg
     9001 gtttccatca acccctgtgc gccgaagctg gcggcgattc gttgttcgat gtcttgcatg
     9061 gcgtctcctg aacctggggg tccaggcgcg atggaaccca ctcaaagcat atccgccgcg
     9121 ggatagcgca tcaatgtccc gggcaatggc tcgggcaagc gctgcctgag cagccagtcc
     9181 gaggcgagcg gccagagcga ctcccggaac cgcttatgga agaaggcgaa atggcctatg
     9241 gcgtcctgct cgaccgcgct cggcgccagg cgcagatggt ggcgttcgct gtgcgtgtag
     9301 tagccgagca gccggtcgat ggcgggcacc gtgccgaaag ggtcgtcgtc gatgccgagc
     9361 gcaagcatcg gcgcgcgtac ctggccaaag cgctggccga agcggcccgc catggcttgg
     9421 ccgtcccggc gcaaggatga ttcgaagcgc ggccccatgc gcgcccaatc cagcgccacg
     9481 cccttgggcg tgtcttccat ccagcccagc cgcttggcgg gcacgtagcc gaacagccgg
     9541 acaatcagcg gcatgacgat gtgccagcgc aagaacatgc gcgaacgcga atccggcgcg
     9601 tagtcccgcc agtaggcgaa ctgcgcgccc atggtgaaga tccggtcgat caagtgcgag
     9661 gaggcggcca ggccgatcac gaagccgccg atggaatgac cgaccacccg gatcggctgc
     9721 cccgggaaat tggcggccac gtaccgaagc acgccctcga agtcgcgttc accccaatcg
     9781 agccaccccg ccgacagcgc gcgcagcgaa ccatgccgcg attcgcctat gccgcgatag
     9841 tcgtaagtca gcacgtcgca gccttgctcg tgcagtgcgt ccgcgaaccg ttcgtagtag
     9901 cggcatcgaa ccgatgtcgc gctattgacg atgacgacgg gacgcggcgg cgagcccgcg
     9961 cgccgccaca cgaatccgcg cagcgcataa ccgtcggtgg cagggatatg gacagggtcc
    10021 cccatggcgg cgcggcgcgg gcctgcctac tcgggatacg tgcccggcac caggatgctg
    10081 cgatccacgt ccgcgatgtt cttgcggccg cacagcgcca tggtcacgtc catttccttg
    10141 cggatgatct ccagcgcgtg ggtcacgccg gcgcggccgt aggcccccag gccatagagg
    10201 aaggagcggc cgatcatcgt gcccttcgcg cccagggcca cggccttgag cacgtcctgg
    10261 cccgagcgga tgccgctgtc gagccagact tcgatcttcg agcccgccgc ctccaccacc
    10321 gagggcagca cctcgatggt cgagcgggcg ccgtcgagct ggcggccgcc atggttgctg
    10381 acgatgagcg catcggcgcc gctatcggcg gccaggcgcg cgtcctcggc gtccaggatg
    10441 cccttgagga tgagcttgcc gccccagcgc tccttgatcc aggccacgtc gtcccaggac
    10501 aggcgcgggt cgaactgctc ggccgtccac gacgacagcg aggacaggtc ggtcacgctc
    10561 ttggcgtggc ccacgatgtt gccgaaggtg cggcgcttgg tgcccagcat gcccaggcac
    10621 cattgcggct tggtggccag gttgatcagg ttgcgcagcg tgggcttggg cggcgccgac
    10681 aggccgttct tgatgtcctt gtggcgctgg cccaggatct gcaggtcgag cgtgagcacc
    10741 agcgccgagc agttggccgc cttggcgcgg tcgatcaggt tcatcacgaa ctcgcggtcg
    10801 cgcatcacgt agagctggaa ccagaaaggc tgcttcgtgg cggcggccac gtcctcgatc
    10861 gagcagatgc tcatggtcga cagcgtgaag ggcacgccga agctggccgc ggcctgggcg
    10921 gccagcatct cgccgtcggc gtgctgcatg ccggtcaggc ccgtgggcgc cagggccacg
    10981 ggcatcgcca cgtcatggcc ggccatcgtg gtgcgcagcg agcggccgtc catgtccacc
    11041 gccacgcgct ggcggaactt gatcttctgg aactcgctct cgttggcgcg gtaggtgctt
    11101 tccgtccagg cgccggagtc ggcgtagtcg tagaacatgc gcggcacgcg cttttgcgcg
    11161 atgatgcgca gctcttcaat ggtggtgacg gtggaaaggt tctggctcat tgtggttttt
    11221 cgcttggccg ccaaaaaggc aaaatattgg aagaaagtat aaatcgtcgg cctgacggcc
    11281 gccgcgccgc accccttgcc aggagccccc tatgtccacc ccccacctcg aaaccctgct
    11341 cgtcaccgtc cacggcaaag tgcagggcgt gggctaccgc cacgccaccg tgcgccacgc
    11401 ccacctgctg ggcgtcaccg gttgggtgca gaacctggaa aacggcacag tcgaggccct
    11461 ggtgcagggc acgcccgacc aggtcgaccg catgctggag tggctgcgcc gcggcccgcc
    11521 ggccgccgtg gtcagcgagc tcgagaccga gcgccagttc atcgacaagc gttatgggcg
    11581 cttcgagcaa gtgtaggaaa gggatgggga aaggaacggc ggctggctag aggctcggcg
    11641 cccgcttcca gaactgcgtg gcctgctcga aggcgtgcgc cagctgcagc acgccccatt
    11701 cgtcgcgata gcgccccacg atctggatgc ccaccggcag gccttcggcc gtaaagccgc
    11761 agggcacgga aatcgccgga tgcccggtca tggtgaggta gtagctcgag cgcatccagt
    11821 cgatgtagtt ccgcatgggc gcgccgtcga tctcggtggg gtattcggtc gccaccggga
    11881 agggcgccac ctggctcacc acgccgacca tgaattcgta ctgccccatg aaggcatgca
    11941 tgcgctcgaa cagcgcggcc cgctcgcgct cggcatggat caaggccgtg gccggctggc
    12001 tcaggcccag ctcggtgttc cagaccacgg tctgcttgag cttgtcgcgg tgcttgcgca
    12061 gggtctcgcc ctgcgatacg gcgaagttct gcgcgcgcag cgcatggaag gcgccgtcgg
    12121 catcgccgaa atcggggcag gcttcttcca ccttgcagcc cagctgctcg aacaccggca
    12181 attgcgcctg caaggcccgc agcaccgccg gctccaccgg cagcccgccc cacgacaggg
    12241 cgcaggcgat gcgcgtgccg gcgaagccgc gctcgagcga acccgcgaac ttggcggggt
    12301 cctgctcgat gctatagggg tcgcgcgcat cggggccggc cagcgcggcc agcatcagcg
    12361 cagcatcggc caccgtgcgc gccatggggc ccgccaccgt cagcgtgctg tacggcgtgg
    12421 ccgaaggcca ttgcggcacc cggcccggcg aaggccgcag gcccaccaca ttgcagaagc
    12481 tcgccggatt gcgcagcgac ccgcccatgt cggtgccgtc ggccaggggc agcatgcgcg
    12541 cggccagggc ggcggccgcg ccgccgctgc tgccgccggg cgtgcggccc gggtcatagg
    12601 ggttgcgcgt ggcgccgaac accgggttga aggtatgcga gcccgcgccg aactccggca
    12661 gattggtctt gcccagggtg atcgcgccgg cctcgcgctg gcgggtcacc ggcaggctgg
    12721 tgcgcgcggg cacgaaatcg cggaacaccg gcgagccgta ggtggtgcgc atgccggccg
    12781 tgagaaaact gtccttgtgc gccaccggca ggccgtgcaa gaggcccagc ggccggctgg
    12841 cggcttgcgc ggcgtcggcg gcgtcggcct ggcgcagcgc gccctcggca tccagcgtga
    12901 cgatggcgtt gaccgccggg ttgcgggcct cgatgctggc caggtgcgcc tcgaccgcct
    12961 ggcgcgcgct caggtgcccg gtttcgattt caacgcgcat ctgcaacgcc gtcatatcgc
    13021 atatcgactt ccaatccata ctggcctcca tgtgccgggc cactatactt cttactgcac
    13081 ccatggccta ctccaaacac tccctcgcgc ggcgcctcaa actgcagcag ctggccatct
    13141 tcgaaagcgt gctggcctgc ggctccatgc tggccgcctc gcgcgagctg cacatgacgc
    13201 agcccgccct gagcaaggcc atccaggagc tggaagagca tttcggccag ccgctgctga
    13261 tccgcacgcg gcgcggcgtg cagcccaccg acttcggcct gatgctgcgc aatcactcgc
    13321 agtcgctcat gaccgagctg cgcagcctgg ccgacgagct caatgcctgg aactcgtccg
    13381 aaacctggga tgccgaggtc agcgggcacg tggcggtggg atcgctgctg gcggcctccg
    13441 cccacctgct accccaggcc gtgctgcgcc tgcgcgagac cacgcccaat gtgtcggtgc
    13501 aggtgcgcgt gggcgtcaac gacaagatgt tcccggagct ggtgcgcggc gagctggacg
    13561 tgatggtggg cctgatgccc gaccacgccg acgacccggt cttcgagcac gtcaagctct
    13621 acgacgagac gctctgcgtg gtggcgggcc gccagcaccc gctggccacc agccagaaca
    13681 tcgatccggc ccggttcgag gccgtggact ggatactgcc cacccccgac accgaggcca
    13741 tgcacgcggt cgacgcgctc tttgccgagc tgggttttcg caagcccgcg cgcatcgtcg
    13801 agtcggtgtc catcatgacc aatctgggcc tcctggtcga atcccggatg atcgccgtga
    13861 tgcccttcag cgtggcgcgc aagttcgtgc acctgggggt gctgagcatc ctgccgctgg
    13921 gccgcgagat ccccttcggc cccatcggct acaccgtgcc gcgcggccgc cccgcctcgc
    13981 ccgccgtcca gcgcttcctg atggcgctca aggacgtcgc cgtggaataa gccgcgccca
    14041 tcgcaaacgt cagcaaaccc cattcccttc ccacccatgc gcttgcaaat cctgtccgac
    14101 ctgcatctcg aggccaatcc cgatttcgcc tttacgccca tgccgggcgt ggacgtgctg
    14161 gtgctggcgg gcgatatcgg ttcgtaccag gaaggctcgg cgctggacgg cccggacttc
    14221 gggctggagc gctacacgcc cctgggccgc gcggcggact ggcccaaggt gctgttcgta
    14281 cccggcaacc acgagttcga cggcctggat ttcgacgcca cgacgcagcg cctgcgccag
    14341 acctgcgagc gcctgggcat cgcctggctg gagcgggagg tggcggtcat cgacggcatc
    14401 cgctttgtcg gcaccacgct ctggacggac ttcgaggccc tggccgcccg gcagcacacc
    14461 gaaacccgcc gccagcagca gcgcgagaag gcctatcgcg cggccaactt ctacctcaac
    14521 aagaacacca ccttcctcga cggccagccc atgctggccg aagacctgcg ccagctcggg
    14581 ttggactgcc agggctggct gcgccaggcc ctggcccagc cctacacggg caaaacgata
    14641 gtggttactc acttcgcgcc gaccttgcga agcgccgacc cgcgttacgg catgacgccg
    14701 ggcacggcgg gtttctgcaa cgggctcgat gacctgctgc cctacgccga cctgtggata
    14761 cacggccact tgcattgccc gaacgattac cgggtgcccg ggcagcgcga cgggcgggcc
    14821 gtgtcctgcc gcgtggtggc caaccccctg ggctatgccg acaagggcga gcaggaaggc
    14881 ttcaagccgg actgggtgat cgaactgccc tagctgcccc ctggtattcc atatatttat
    14941 atttccgccc agggaattca ttattcaagc tcccccgcgc ccgatacatt caaggcatag
    15001 ccggatacaa cggggccgtc gacggaaacg gcccatgcat ggggaatttg aaattggcaa
    15061 cgttcgatct gcccgcatcc ctgcggccga cccgcaacta cgtcggtggc gaattcgtcg
    15121 ccactgataa aactttccag aatatcagcc ctatcgacgg ccggctgctg gccgaggtgt
    15181 gcgaggccga tgccgccctg gtcgaccgcg ccgtgcgcgc cggccgcgcc gcgctcgaag
    15241 gcccctgggg cgccatgtcc gtcgacgagc gcgcgcgctg cctgcacaag gtcgccgaca
    15301 tcatcgagcg ccgcttcgac gacttcctcg ccgccgagat cgccgacacc ggccgcccgc
    15361 tcgcccaggc gcgcaagctc gacgtctacc gcggcatcgc caacttccgc accttcgccg
    15421 acctgggccg gctgcgcggc agcgagttct tcgagacccg cctgggcgac ggcacgcagc
    15481 tcatcaacta cgtcacgcgc aagccgctgg gcgtggtcgc catcatcgcg ccctggaacc
    15541 tgcccatcct ctcgctcacc tggaaggccg cgcccgcgct catctgcggc aacgccgtgg
    15601 tgtgcaagcc ttccgaggaa acgccgtcgt cggccaccct catcgccgaa gccttccacg
    15661 aggccggcgt gccgcccggc gtcttcaacc tggtgcacgg cttcggcccc ggctcggccg
    15721 gcgaggcgct caccacccac cccgggatcg acgccgtggc cttcaccggc gaatcgcgca
    15781 ccggcgcggc catcatgcgc gccgtggccg acggcgtgaa ggacatctcc ttcgagctgg
    15841 gcggcaagaa cgccgccatc gtcttcgccg acgccgactt cgacgcggcc gtgcagggca
    15901 tcgtcgcctc cagcttcacc aacgcgggcc aggtctgcct gtgctccgag cgcgtctacg
    15961 tcgaacggcc catcttcgag cgcttcgtcg ccgcgctgaa agaacgcacc gaggcgctca
    16021 aggtgggctg gcccgacgac gaggacgtgt tcatgggttc gctcatctcg cgcggccatc
    16081 gcgagaaggt gctctcctac ttcgacctgg cggtgcagga aggcgccacg gtggtcgccg
    16141 gcggccgcgc gccggtattc catgatgcgc gcgacggcgg ggcctatgtg ctgcccacca
    16201 tctggacggg cctgcccgac accgcgcgct gcatgcgcga agaagtgttc ggcccggtct
    16261 gccatatcgc gcccttcgac agcgaggacg aagtcgcgcg ccgcgccaac gacagcgcct
    16321 acggcctggc cgccagcgtc tggaccacca acctgtcgcg cgcgcaccgg ttcggcccca
    16381 ggctgcacgc gggcatcgtc tggatcaaca cctggttctc gcgcgacctg cgcacgcctt
    16441 tcggcggcgg cgggctgtcg ggcatcggcc gcgaaggcgg cgagtattcg ctgaatttct
    16501 tctccgacat cagcaacatc tgcatcaagg tataggcatg agcgacaagt ctgcagacca
    16561 aagcgccggc gtcatcgcgg gcaaggccgt gcctcgcggc aagttcccgc actacaagcg
    16621 cgcgggcgac ttcatcttcg tctcgggcac cagctcgcgc cgtcccgaca acagcttcga
    16681 gggcgtgcag gtcgacccgc tgggcgtcac caacctcgac atccgcgcgc agacgcgcgc
    16741 cgtcatccac aatatcggcg agatcctggc cggcgccggc gccggcctgg ccgacctggt
    16801 cgaagcgcag gtcttcctgg tcagcatgaa cgacttcggc ggcttcaacg aagtgtgggc
    16861 cgagttcttc gactacaccg gcccgacgcg caccacggtg gccgtgcacc agctgccgca
    16921 tccgcacctg ctcatcgaaa tcaaggccac ggcctactct ccgcttggta ctcacccata
    16981 acgaaaggag atacggatgt ccacgctcca atacggctac ccattcaact tcaagcgctg
    17041 gctcgacgac aacgcccacc tgctcaagcc gcccgtcggc aaccagcaga tctggaaaga
    17101 cagcgacttc atggtcaccg tggtcggcgg ccccaaccag cgcaccgact tccacgacga
    17161 cccgctcgaa gagttcttct accagttcaa gggcaacgcc cacctgctgc tctgggaccg
    17221 cggccgctac gagcgcgtgc acctgaaaga aggcgacatg ttcctcatgc cgccgcacgt
    17281 ctcgcattcg ccgcagcgac ccgaacccga cagccgctgc ctggtcatcg agcgcgtgcg
    17341 ccccaagggc cagcccgacg ccctgcactg ggactgcgcg cactgcggca cggtcgtcaa
    17401 gcgctacgaa ttccagctcg agaacatcgt cgtcgacctg ccgccggtct acgagcagtt
    17461 ctatggaact tccgaggccg agcgcgcctg cccgcaatgc ggccatgtgc acccgggccg
    17521 cgactaccag acctggcacg cggacctgaa cgccaggcat ccggatctat agcgcgcacg
    17581 gcatgggcac ccacacacac ataggcacct cggcaatgat cgacatccac tcccacttct
    17641 tcccggaaat cccgcaagcc ctggcccgcg agttcgatgc cgaacgcgcc gtctggctgc
    17701 gcgtcgacga gggccaggac accggccaga tcatgctggg cgacaagccc ttccgccccg
    17761 tctaccgtgc tctgtgggac gccggctacc gcgtcgggga actcgacgcg cagggcgtgg
    17821 ccatgcagct cacctgcgcc acgccggtga tgttcggcta ttcgtgggac gcggccaagg
    17881 cagccgaatg ggccgcccgc atgaacgacc tggccgtgga cttctgcgcc gcgcatccca
    17941 cccgcctgaa ggccctgtcg caagtgccca tgcaggacat caagctcgcc accgccgaag
    18001 ccaagcgcgc ccagctgcgc ggctgcgtgg gcgtgcagat cggcaaccat gtcggcgaca
    18061 aggacatgga ccaccccgac atgatcgact tcctcacctt ctgcgccgag gaaggcatcc
    18121 ccgtcttcgc ccacccctgg gacatgatgg gcggccctgg ccgaatgaag aagtggatgc
    18181 tgccctggct ggtagccatg cccgccgaaa cccagctggg catgctgtcg ctgatcctgt
    18241 ctggcgcgct cgagcatatc cccgaaacgc tgaagctctg cttcgcccac ggcggcggca
    18301 gcttcgccta cctgctgggc cgcgtcgaca acgcctggca gcaccgcgac atcgtccgcc
    18361 aggactgccc gcgcccgccg tccgaatacg ccaggcgcct gtggtgcgac tcggccctgt
    18421 tcgaccacaa ggccctggac atgctggtcg acgccgtggg cgtggaccac gtgatgatgg
    18481 gatcggacta tccgtttccg ctgggcgagc agcccatcgg gtcgctggtg tcaggcagcc
    18541 cgtacctgga cgaagcggcg cgcaagaaga tcctggccga caacgccaag gtttttttca
    18601 acctggcttg agaggtcgca ctcaagagat cgcatcgccg tgcaaatgac gtcgggccgt
    18661 tgatcgaagc cccgcgcagc caaagttcaa tcgtacgaaa aacaagggga attaccgcat
    18721 ggaaccacaa gccagtacgc tgagcgccgc gaaacgccga accatctatc tgctctgctt
    18781 tctcgccatc atcatcgatg gattcgacac aaccaccatc gcctatgtgg cgccgcgcct
    18841 ctcggccgtg tggcagctcg cccgggggga gatcgccccg gccttcatcg ccaccagcat
    18901 aggcgcggtc atcggctatc tgctgagcgg ctgggccgcc gcccgcctgg gcaagcgaaa
    18961 gctgatcgca tggagcatct tcgtcttcgg cgtgctcacc attgccaccg cgtatgtcgg
    19021 cacgctgacc gaactgacgg cgatccggct tttcacggcg atcgccctgg gcttcgtcgt
    19081 ccccgccgcc atctcttccg cggccgacca tagcgacgac cgcacgcgcg cggccgtgac
    19141 gatcttcgtg accaccggcc tgtcgctggg cgcggcggcg ggaggattcc tcgccgcgtt
    19201 cctgatggtg cggttcgact ggcagtcggt ctttataacg ggcggcgtac tggcgttctg
    19261 tctcgtcccc ttcgtctggc agtcgctggc gaccgatggc ctggagtccg agacggcaaa
    19321 aacggcgaac ggcgggctgg cggcgctctt caaagaagga caggcaagct ccacgatcct
    19381 gatctgggcg ttggccttcg tctctttcat ggtgacgtac ctgttcatgt tctgggtgcc
    19441 cacgctgctt accctgttca agttctcgcc gtcccaggct cccctcggat cgggcgcctt
    19501 cggcctcggg ggcgtgctcg gcaatctcgt catcctgcct tttgtgaaac ggtacggtcc
    19561 ggaacgactg gtgatcgcca cgtcgctgct gtgcatcgtg tgtctttacg ccctggccat
    19621 cggcgccttg caacccatgc tggtgctggt cgcgatcgcg ggcgtggggg cgggcctggt
    19681 gagcgcctgc gtcagccagt ccacgctttc ggtgctccgc tttcgcgccg atttgcgcac
    19741 ctcggccgtc ggggccgccg cggccgttgg ccggatcggt tccatcgtcg gtcccgcgat
    19801 cggcggcatg ctcgtagcgt cgaagctgag ccccgagatg atcatggtcg tctcctcggt
    19861 cccggtggtc ggcatcatcc tgattcttat cggcctgttg gcggtggcgc gcccggcgct
    19921 gccgctctcc aggcaagccg gctgacccgt ccacgcgaac aaggcccctt caccatgcaa
    19981 agcaagaacc cgaccgtcga cacgccagcc ggcatcatcc atggcgccgc cgttggcaga
    20041 gtccatgctt tccaagggat ccggtatggc gccaacgccg gccggttcaa ggcggtcgtg
    20101 cccatcgcgc aaagcggcgc atcgccgcaa gtgccgccat gcgccttccc gcagaacaaa
    20161 aaccggctcg agttcgtgat gggcgatgcg atccacggcc ttccccaggc cgaggacgca
    20221 tttcttctgg acctttgggt tccggaaaac cacgccggca aactgcccgt actcgtgttc
    20281 atacacggcg gcgcgttcat ttccggagga ggatccggca cgcgctacga cggggccatg
    20341 tttgcgcaag agcagtcggc catcgtggtg acgctcaatt accggctcgg cgccctgggg
    20401 cacctggcca tcgaccgtgt cagcgacggc aatctcccgt ttgacgacat cctctgcgcg
    20461 ctcggctgga tccgggacaa catcgcccgc ttcggcggag atcccgcgaa cgtgaccgtc
    20521 gcgggccagt cggccggcgc atggtacgcc tacctgctgg gcgtcagccc agccgcccag
    20581 ggcctgctgc gccggaacgt cctgttcagc ctgccgttct tcccgcccat ggcgcaagcg
    20641 gcggccgcgg aacttgccgc ggcctttggc gagatgctcg gcggcgcgga tccgtccgag
    20701 gccgccgtgg aagacctgct tgccgtgcaa ggcaaagtca tgcggggaac cgcgcggttt
    20761 ggcgacttga cgctgggctt ctacccggtc gccagcgacg ggaaagttcc cgcctcgctt
    20821 ttcgactatg cgaagtcggc agccgatgcg catatcgagg gcacgctggc aggctacacg
    20881 cgggaagaaa acgcggcctt catgatccag gacccgcgca cccggaatcc ggaccccgcc
    20941 gcggtcgacc aatggttccg ggggcacttt ggcgccatgg gcggcatcaa gcgttcctac
    21001 ctcgactcgc tcaggcctgc gccgactccc tatacctccc tggtcgacgg cggaacgcac
    21061 atcagcttcg cctcgcccac ggttcaagtc gccgccagct tcggcaggcg cgggctgaac
    21121 agctggctct accgcttcga cctgcaatcg ggcacgccag acctccatag cccgcattgc
    21181 atggaactgc cattcctgtt tgcgaacagg cggcagtgga ccgacgccgc catgcttgcc
    21241 ggaatcgatg accgcgcgtt cgatcgtgcc gggcggtgtt tccgccatgc gatcggcagc
    21301 ttcgcgcggg gcgacgcgcc ggggttcacc cctgcggacc cgtgggcaca gttcagggag
    21361 ggcgcctgga cttgccatct cttcggcgat acggaccacg ccggcacaat ctcgctcgag
    21421 ccgtccgcct ttatcgaaaa ctgaccgcgg cgcgatcagc ccgggaacca gaccccggcg
    21481 tccttgctcg ccggattggc ggggtcgaag tatttcttct gccagctgtc gtcgggcgac
    21541 agcgcgctga acgccttgct gctggaccgc gacacgttgt agcggaaccg gtaatggctg
    21601 tagacatcca ggacgtgggc ggcataggcc agggccaatg gctggtggcc ctggacgatc
    21661 atcaggttct cgtcgttgtt gtaggacgcg cgaaagccct ggttatggct gccggtcgcc
    21721 accacgcagc gctccgacat gggatcgatg acgacgatct tgtcgtggat gatggcgtgc
    21781 gcgccgtcgg acgatttgag caactccttt tcccagtcgg catgcgggct ggtgatcgcc
    21841 atggccgaga ccacgtcgtc gccctcgccg ccgcgatgcg tcagctgcgt ggcgaacgcg
    21901 ccgggcgact tcctgtcggt cgtcgccccg cgcacgaaaa gcccgggcct ggcatccagg
    21961 caggcggccg cctggtcgat gacgctcggc gttcccggct cgaactccag aaaaagaatc
    22021 gcctgctgtg cctgcgccat cagcgtgaac aggtcggcga ggtcgggcgg cgtggcctcg
    22081 ttcttcttgc gttggccgct ggcgcgcgca tgcggcgtgt tgggcgagaa ccagacggtg
    22141 gccttgcctt tgtccacggg cacgtccttg gcgccgggcg tcgcgttcga tgaccgcagc
    22201 gccgcgccct ggcgcgaatc gtcctcgagg ctgtcgtcgc gcagccgccc ccagtagtcg
    22261 aaataggcgc ttgccaggac ctcgtcctcc acgaccaggg cattgttgga ctggccgcac
    22321 aggcccgtat tggtccagtt ggtcgacccc agcaggacgg cctgcggcac cttggtggcg
    22381 tcgacataga cgcagaattt attgtggccg atatgcccgc tcggcacgaa gcggtcgatg
    22441 acctgcgcgc ccttcctgcc atgcaggcgc tcgcgcgccg gcgcgttctc gctgtccttg
    22501 gaaccggtgt tcgaaagaat caggtgcagg ttcctcaccg ccatcaggcg gtcgatcagc
    22561 tcctcgtcct gcagctcgta gagcgccaga tagcaggcgc cgccctggtg cgccgcccgg
    22621 tcgagcaggg acgtgacgcc cgcgatgatg tccccggcca gcgcggtccg gatcctgtcc
    22681 ccgggcgtgg cgatcgcctt gacgagcacg gcggagtccg gcgtcccatt ggcggtttcg
    22741 ggcagctggt gcgcgagcca ctgggtcgaa agaatgcccc gattgaagta ggtggagaat
    22801 ttcccccgct tgggcgtcag cgtcaccggc ccggccatca gcgacccggc ctcgtcgagc
    22861 gtcaggggca ggcccgcggc cggatccccc gataccggcg tgatcttgta gagatagctc
    22921 gcgccccgcc tggccgtcag atcgcgccag gcaaaggcct gtaccggcca gatggtcgaa
    22981 tccttctgcg tccagtccg
//
LOCUS       DASWPO010000333        22601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_793011, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000333 DASWPO010000000
VERSION     DASWPO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 22601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22601
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793011"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VGC69_17635"
     CDS             complement(<1..1468)
                     /locus_tag="VGC69_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0297156.1"
                     /translation="MPDQPLRPDGAIDTFQDNELYDPAWACILSALQADLRPKASAIA
                     AMFYEQTGETDTDDIIGMLTTAGLQHLQAQQAQNLLLLASPDLSAERHRATALRIGKI
                     HAIVGLTQKDLLRGQDILHTTLRGLVDANRHGKALGLLGRRLIRDLTWQTEAYQLMQA
                     ARYEVLLNLSQLVWTADNYTDLISQSAELLSQHPEIAACAFGKPDQDGIFQFEFMAGE
                     PAMLTSLVEQPMPSLLEDETHTQGLTGRAWHTKQVQHCRNFETDERLAFWRERAARLN
                     VQSFAAIPICHAEQPPRSILTLYSHYVGGYASADQQSFIRQIQSLLMFACARLEGQAG
                     YSDTVPYAVRQRWARLIRSDALQMHYQPILELKTRRVLKVEALVRLHDGNQVIAPGTF
                     FPALSSDDFFEIYARGLDQSLAQCTRWLAAGLNFCVSVNLPSKALADPRYLQVTRQTL
                     ARHGCPAGNLVLEILETDALPRDVDVAVALGRFKALGVG"
     gene            complement(1591..2667)
                     /locus_tag="VGC69_17640"
     CDS             complement(1591..2667)
                     /locus_tag="VGC69_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297157.1"
                     /translation="MPLSARPPLSINGLSTLLRRGACMALAAAGLLADASAQAATPCA
                     PPRHGFNLVQDDRAPLGSAQAEASLKQLRGTGANAVAIVPFFRQPRSDSTDVSRGSDM
                     SDGELARAIRTAHKLGLAAIVKPHVQIPDGDPGDVRMASDDDWAQWFHHYEQALAGLA
                     AVAKDEHAEALVIGTELDQSLDRPEWPDLIARLRRIFHGKLVYAARGAEGAERVPFWN
                     KLDAVGATLYPTLGADNDPDSWRAAMASERDHLLALAKQAKRHLFIAEIGIRSASGAA
                     ARPGQRPDENGAPADEALQAKVLATWMAELSDPAIHTVLVWRWNSDPDSGGASDTDYT
                     VQGKSAERALTKQWRSCSRTVAVW"
     gene            2843..3235
                     /locus_tag="VGC69_17645"
     CDS             2843..3235
                     /locus_tag="VGC69_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0297158.1"
                     /translation="MTIEVRANQPASALRYTVTAEGHGMPLGFPRPDGDGPDPHDYFD
                     GALGGCKALTLMWYAQRKQLPLTSVDVDVVRDAGEEKRGVYKLTARLRLNGDLSEAQR
                     AELLAVAEKCPIHRLMATADVQITTELA"
     gene            complement(3284..4069)
                     /gene="fabI"
                     /locus_tag="VGC69_17650"
     CDS             complement(3284..4069)
                     /gene="fabI"
                     /locus_tag="VGC69_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY0297159.1"
                     /translation="MGFLAGKRILVTGVLSNRSIAYGIARACHQQGAELAFTYVGDRF
                     KDRVGEFAAEFGSSIVLPCDVAEDAQIEGAFAELGKHWDGLDGLVHSIGFAPREAIAG
                     DFLDGLSREAFRIAHDISAYSFPAMAKAALPLMQGRPASVLTLTYLGAEKVVPNYNTM
                     GLAKASLEASVRYLATTLGPKGIRANGISAGPIKTLAASGIKDFSSILKFVEANAPLR
                     RNITTEDVGNTAAFLLSDLAAGITGEITHVDAGFSTVIPGMEG"
     gene            complement(4137..5558)
                     /locus_tag="VGC69_17655"
     CDS             complement(4137..5558)
                     /locus_tag="VGC69_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0297160.1"
                     /translation="MTLIRNLLLLLLVLLAGCAGIDRQADQTSAMGVRIARNTSRTID
                     LTHPPHDAWDRIRRGFAIPNLDNDLSQQWTEYYAAHPEAVERMAQRAGKYLYFIIDEV
                     NRRGLPTELALLPFVESAYNPTAQSPAQASGLWQFVPATGEHYNLKQDWWRDERRDPI
                     ASTYAALDYLAELFEMQGDWYLALASYNWGENAVQRAIARNQSLGLPTDYLSLDMPDE
                     TRNYVPKLQAIKNIISNPEKYAVTLPPVENTPYFAMISEPGDMDLEIAARLAEMPLDE
                     FKALNPSYKRPVINGQENSTLLLPKDKVEVFNHNFANYQGRLSNWRIYQPQRGEKVAS
                     VARHLGISELRLRQLNGIAPHAKTVSGQNLLVPAGDVGDSAPLALAAATDGDSEPVDP
                     PPAHAKTATGRKQSADHHASRARQTRGTARKGNAKAIGKLADKGRAQAAGRGGSKRGA
                     QGTKGGRAVKVASADKKHPAKRH"
     gene            complement(5563..6381)
                     /gene="gloB"
                     /locus_tag="VGC69_17660"
     CDS             complement(5563..6381)
                     /gene="gloB"
                     /locus_tag="VGC69_17660"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY0297161.1"
                     /translation="MKPSIPSSQGAELLPVPAFTDNYIWTVIRDGLAAVVDPGEAGPV
                     LQCLQARGLELRAILLTHHHGDHVGGVMELSRLTGATVYGPSGEALPRCDVRLAEGDH
                     VVLPELGLDLAVLDVPGHTAGHIAYFGRIGQEAGQKPVLFCGDTLFMAGCGRLFEGTA
                     EQMDQSLAKLATLPGNTQVCCAHEYTLSNLHWALAVEPNNRATQAAQEQARRQRENNL
                     PTLPSSIGQELQINPFLRTRHPMVVQAASAWAGRPLTSPVNVFAALRQWKNEFK"
     gene            6393..7151
                     /locus_tag="VGC69_17665"
     CDS             6393..7151
                     /locus_tag="VGC69_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0297162.1"
                     /translation="MAEQMLPIVELAEWFQTPPGQYVLAWERAQFDERVADVFGFQAW
                     QVGLPGLDLLRANRMPFKAVVGAEIAIDDATIGWRNGVAALPEALPFDSQSLDLLVLP
                     HAFECTDSPHQVLREVERVLVPEGRVVISGFNPWSLWGARNAMPGMEAWLPRPVSSQV
                     SPLRMKDWLKLMSFEVDATRFGCYVPPCRTDQWLRRWHFLEAPGQRWWTLGGAVYVMS
                     ATKRVIGMRIIGPAWKKKRQSASTASVAVHRSEL"
     gene            7196..7666
                     /gene="rnhA"
                     /locus_tag="VGC69_17670"
     CDS             7196..7666
                     /gene="rnhA"
                     /locus_tag="VGC69_17670"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY0297163.1"
                     /translation="MQSSEIQDADRVEMWTDGACKGNPGPGGWGVLMLAGRHRKEMHG
                     GEAQTTNNRMELLAVIEGLRVLKRSCTVVIHTDSQYVMKGMTEWLANWKRRGWITADK
                     KPVKNAELWQALDEQVSRHQVSWRWVKGHAGDPGNERADALANLGVDSVRQRAG"
     gene            7705..8436
                     /gene="dnaQ"
                     /locus_tag="VGC69_17675"
     CDS             7705..8436
                     /gene="dnaQ"
                     /locus_tag="VGC69_17675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY0297164.1"
                     /translation="MRQIVFDTETTGLRPEDGHRIVEIGCVELVNRSLTGKTLHFYLN
                     PGRDSEEGALAVHGLTTEFLADKPRFADVADEFLAFIQGAELIAHNATFDVKFFNAEF
                     KLIGRGEIAQYCESITDSLLHARAMFPGKRNSLDALCDRFGISNAHRTLHGALLDSEL
                     LAEVWLAMTRGQDALLIDTDDAAPGAGAGNSSLGAFDASVLPVIAAAADELAEHAAYL
                     KALDKASGKTVWRAYEPLAAEGDAA"
     gene            complement(8400..9599)
                     /locus_tag="VGC69_17680"
     CDS             complement(8400..9599)
                     /locus_tag="VGC69_17680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY0297165.1"
                     /translation="MKHAASHWLNRERFTRYPRLFLLLFIVLGIAWALLAKNHVDLKG
                     KPLGYDFITFWAASYLGLHNHAPLAYDLASIFRAEQAAVPASNSVFVWYYPPTFYVLI
                     LPLALLAYFPAYWTFMLLTLAAYVCAMRRIVRTPDAMWCLAAFSGAWMNLFHGQNAFL
                     TAALAAAALLCLEKRPVLAGVFIGLLAIKPHLALLFPIALIAIGAWRTFVVAAATAIL
                     FMVISTAIMGTDTLMASVHSLGYARLFLETGTLPWPKMPTVFAFLRLLGTPVSLAYAV
                     HAVVALVAIRTVWRIWRGSQDQRLRAAALMTGTFLVSPYVFDYDLAWLAFPIAWLALC
                     GLETGWRTAEREILAAAWLLPLIMAPFATPTHIQLGPIVLGALLWITWRRARSPYAAS
                     PSAASGS"
     gene            complement(9618..10181)
                     /locus_tag="VGC69_17685"
     CDS             complement(9618..10181)
                     /locus_tag="VGC69_17685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0297166.1"
                     /translation="MKALDKLLRRWRSDIALRAAPKKLSHVFDIGCDDGYLLKRVANR
                     SAILDGCDPRLAAQPMTAGSVLLQGCFPSAWEEAGLHRKYDVVFALAVLEHFSEQDLI
                     GAARAIAQMLADTGRLVVTVPAPIVDKILDVLMFLKLIEGQAVEEHHGFDPASLVQIF
                     SDHLNLVSHKTFQLGLNHVFVFKKIAS"
     gene            complement(10168..11340)
                     /locus_tag="VGC69_17690"
     CDS             complement(10168..11340)
                     /locus_tag="VGC69_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0297167.1"
                     /translation="MEFSILMPCLNEAETLAACIEKAQDWLQRTGALGEIIIADNGSH
                     DGSQEIARKHGARVVDVALRGYGEALYQGAMAARGEYIIMGDSDGSYDFGALDGFTEK
                     LVQGYDLVVGNRFLGGISHGAMPWKNRYIGNPLLTRIGRLLFNCPVKDMHCGLRGFSK
                     QAFLRMDLRTTGMEFASEMIIKASLLDMRIAEVPTRLSKDGRTRAPHLRPWRDGWRHF
                     RFMLLFSPRWLFLIPGLITMFVSIFFYLAIFAGQLKIGGVVFDINTLFYAQTGITVGL
                     IAIMLGLAVRMFGMYEGLLKKNRMFETLRAKPILEIGGILGIALAIFGGYLGVRLLKN
                     WSSAGFGVLQQGIFLKTVSLSTLSMTLGGIIFLMSLIMGFLALPIRRDVEKNHEGP"
     gene            complement(11344..13230)
                     /locus_tag="VGC69_17695"
     CDS             complement(11344..13230)
                     /locus_tag="VGC69_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297168.1"
                     /translation="MRWGIWLIAFAIVIGACVASTGAFIVDEVVYQAGVDAFQHHHTL
                     ILENGYGTFRAESLTLWLLAEGPHGLTPQYPVGTAIAGGVLAPLLGARSLIFFNALAS
                     VGTLFLVRALARILFNREEIAWRAVLILALATYWPEFTVGIWPHAISVFGATATLLCA
                     WLAITRDSQNFRWATLSGLCMGLGLLFRLDAILCLPIAGLATMLFAPRRAGILLAGCL
                     VGILPGLITTSLTNQFKFGTYNPFSYGNTVAVAIDNATLSGQTGAILLVLLLGATLIA
                     LHWLHRSKKLGKVHVLASLGLLFVIALAIPATREVLSHYMTGAQGLIWDATTIIDPHD
                     GVVKGAYGTLSFWGMAKKAWAQSLPWLGLLFFLVCSRWKDADREPIAFILMGITLWTL
                     PFILHLYHGALGSNMRYFLPALPLAAILAAKVWEDLIVVAGQGGKTTHLATLGRWAVI
                     IVFVAWSFAGPATIYGANQIFPNYLFAALGLLCLLAGREGPHRILFARMAIAFGVGGF
                     MAAGIFNIMDLSVSQAHRHQNRLMNEAFAKLPSPSLFLALPEGAMHQIGHPDTLVAVA
                     DPIHGKVDGTLLTQALNKGYRVFVIAPWLAQALASSPQLKDAGEVKELGEPWQDAVHE
                     LVDK"
     gene            13433..14743
                     /locus_tag="VGC69_17700"
     CDS             13433..14743
                     /locus_tag="VGC69_17700"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0297169.1"
                     /translation="MRLSAPPFSADSGWQYLIVLCAIAVPSLALVVPGGANIGYFALA
                     AAVFATCFASGRDAQRMAAPAGGIDLPAILPVSLALPSVAVLWTQLAHWDFDGQPMDA
                     PSRFLLAVPLLYCLQRLPPSRLRRLRHVQWGLCAGAIGAALVLWVAPLDGSMRHVPKF
                     ASAITFGNLGLVMGLGAMLTLGWRLTRSFLEPLLKVVAVLAGFYASLLSGTRGGWVAL
                     PVLLILFLLLSRLRGAYKLLLCVLAVAILSGVYLTDKNVQMRIHTASAEYAAAVQAHG
                     EEKSALAASDTSVGLRLSFWRAGYAMFLAHPFAGVGSQHIQEVYEDRAAHGLMPQDAG
                     HFDHLHNDYLQSLAANGLPGLLARLMAYLVPLYLFVRAIRSAEPQRVTAGWVGALVVV
                     SYMVFGLTETMFSITMNASFYTGLVLIFSVLALPARPGTTGWQA"
     gene            14802..14876
                     /locus_tag="VGC69_17705"
     tRNA            14802..14876
                     /locus_tag="VGC69_17705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14834..14836,aa:Val,seq:cac)
     gene            complement(15094..16365)
                     /locus_tag="VGC69_17710"
     CDS             complement(15094..16365)
                     /locus_tag="VGC69_17710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0297170.1"
                     /translation="MMLVTIMSMAISGSLRHSTVPGVSRWIGANAAAIIALILFALQH
                     DSPTILSVLVANLLLAFASVLGYQGCRQFFHLQPVTMLPYVGCTGLVLGIAYWTYASP
                     NMGACIATVSIFYACMHASIGWTAWKHRAPKRPSYGCCFIVVIACASALGHVIRGLVY
                     GVGLLPQGSLLQSAVSSIAFPMVGMLVLPGLSMGMVILVHDRLAEQLERWANVDDLTG
                     ALTRRRFFAKAQEMLEHATASGARLSVAIIDMDHFKLINDQYGHAAGDSVLAHFGRLV
                     RTTLRETDVFGRVGGEEFAILFSRTGRQDAWSRMEVLQERTNFDRRGMLNDAKDIPPH
                     RYSFCAGIDEYRYSDSLPELLARADAALYTAKTQGRDRVVDAQDVLRPAAAANAAMDH
                     ALDFGLLHQSIDASGQSASPNVRHWTNAPRP"
     gene            complement(16660..18186)
                     /locus_tag="VGC69_17715"
     CDS             complement(16660..18186)
                     /locus_tag="VGC69_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY0297171.1"
                     /translation="MPPADTSAPLNRGMITLSIMLATLIQTLDSTIANVALPHMQGAL
                     SASQDEISWVLTSYIVAAAIATPLSGWLAARYGTTQLLCASIAGFTVASALCGISDSL
                     SQIVTARLLQGLFGAALVPLSQSILLNINPPEKQPQAMAIWGVGVMVGPILGPTLGGW
                     LTDSYNWRWVFFINLPIGALALLGVMTYMPKIRQGERHALDVFGFTTLSLSIGALQAM
                     LDRGQQKDWFNSTEICIEAIVAFVSFTFFLIHTATAGKRSFFRAELLKDRNFVTGSLF
                     IFVIGAILYATRALLPPMLQNLMDYPVATTGLVTAPSGLGTMIAMLIAGRLVGRLDVR
                     GMLIAGLLISGYALYQMMGYTLTLSESDIVWPGVVQGVGLGLVFVPLSAVSFATLKPE
                     LRAYGTAIFSLMRNIGSSIGISIVQTVVTRNSQTVHASLAEHVSRGESGWQQYFDLAT
                     ETGRESVNRIIDQQASMIAYLDAFKLMFIATLCILPLVIFIRRRRSAGSGAAAHAVMD
                     "
     gene            18270..18716
                     /locus_tag="VGC69_17720"
     CDS             18270..18716
                     /locus_tag="VGC69_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0297172.1"
                     /translation="MSNTYEERFGFLVSDIARLLATQFDRRGRQRLVLSRAQCRVALY
                     LSTLGPMSQAKLAETLDVTPMTVARMLDRMEEAGWVQRVQAPNDRRSFLVRVTAKVEE
                     AMGPVLDLGDEITALAMQGLSDEERQTLLRLLSKVRGNLGSSQAEG"
     gene            18882..20066
                     /locus_tag="VGC69_17725"
     CDS             18882..20066
                     /locus_tag="VGC69_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0297173.1"
                     /translation="MIGRDFLHSPGPTHIPQPVLNAMNRQPLDLVDDRLKLLAKSCFD
                     DLKDIFRTKGQVFIYAGNGHGGWEAVLANICAPGDTVLVPVVGHFAHEWADHARALGI
                     KAETIEGDWRQGIDPAQIEQHLRRDTERRIKAVLAVQVDTAASLSCDIQAVRQAMDAA
                     GHPALLVADTVASLAAMPFDMDAWGVDVAITASQKALMCPPGLAIVAANDKAIEAARS
                     TPRFQRYWDWMQRIGDESYTKFCGTMPEHHMFALRTALDLIKQRGLETIFRAQRQLSG
                     AVHQAVEAWSQAGAMAFNVPASSERAVTVTTIRTSAGIDPDRLRTYAREKLGLMIAGG
                     LGKLGGSAFRIGHMGSINEPMLLGCLATLDLALKELGLPHGEGAVQAAIAHLARERTA
                     AA"
     gene            complement(20113..21780)
                     /locus_tag="VGC69_17730"
     CDS             complement(20113..21780)
                     /locus_tag="VGC69_17730"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0297174.1"
                     /translation="MRANSGLSQSLAPPSSAAPSSASWRDALAARFSLDAVSLQPLVL
                     LLLGLQLLLWTVLLGLSHSAPDVDNMEELIWGNVFQWGYYKHPPLPSWVHYGLITLFG
                     RQVWITFFAGQLSVTLALYFIWRLGCEMTSQRNALLAVLLMMPIAYFTTRGVMSNHNT
                     IQLWSVAGAIWMFYRAWRYQQLRDWLLLGVFSALAMLTKYSAGVWFVMFALQLLASGS
                     LRELRTWKGVLAGACVLAVMLLPHAMWLYNMEYVLHNPANPLTYATVATRTDSMTRAS
                     NLLAIYYVFTTTLARIAPMLLAMLIIGGLLRHAARRRPAGEAGGTGGKPGSIAAQLRP
                     QDRRFILMLGLGPFVLTCAASFIADTWMIADWTTTFFLMAGLLTFWLYAEHGGRRLLK
                     TSLAVVGVMQVLTVVGYAAGRGPLPSLMGRPTRSNFPSSQVSRQLQQVWHDHVDGPLR
                     FIAADTWIGGNIAIHAGRQAQVLIDGDPQNTPWIDPGQLAACGMLVVIDASPFNHDPV
                     WPLVAERMKQAQWQGIARLPATRNANGPQVTVDWGIVPPTTGCKPAP"
     gene            22044..>22601
                     /locus_tag="VGC69_17735"
     CDS             22044..>22601
                     /locus_tag="VGC69_17735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0297175.1"
                     /translation="MTTSPLDQSVAVHAAESDPLAPPRSVDGRPPVRGLVTCVVPGLN
                     ESENLKVLLPRLSALLEQWCDGWEILVVDDGSTDDTPALMAEWTKTPGVRYVQLSRNF
                     GKEAALAAGLEASAGDVVICLDADLQHPLELVPRMLQRWQAGIDMVYAVRSDRVDETW
                     VKRVGTWFFYRLLGSTRRMRVPPHAG"
BASE COUNT         4054 a         6868 c         7633 g         4046 t
ORIGIN      
        1 gtcccacgcc cagggccttg aaccggccca gcgcgacggc gacgtccacg tcgcggggca
       61 gggcgtcggt ttcgaggatc tccagcacca ggttcccggc agggcagcca tggcgggcca
      121 gcgtctggcg cgtgacctgc aggtagcgcg gatcggccag cgctttcgaa ggcaggttga
      181 cggacacgca gaaattcagc ccggccgcca gccagcgcgt gcattgcgcc agcgactggt
      241 ccaggccccg ggcatagatc tcgaaaaagt cgtcggacga cagggcgggg aagaacgtgc
      301 ccggcgcaat gacctggttg ccgtcgtgca ggcgcaccag cgcctcgacc ttcagcacgc
      361 gcctcgtctt cagctccagg atgggctggt agtgcatctg cagcgcatcg gagcggatca
      421 ggcgggccca gcgctggcgc accgcgtaag gcacggtatc gctgtagccg gcctggcctt
      481 ccaggcgggc gcaggcgaac atcagcaggg actggatctg gcggatgaag gactgctggt
      541 cggccgatgc atagccgccg acataatggc tgtagagcgt gaggatgctg cgcggcggct
      601 gctcggcatg gcagatcggg atcgcggcaa acgattgcac gttcaagcgt gccgcccgtt
      661 cgcgccagaa agcgaggcgc tcatcggtct cgaagttgcg gcagtgctgc acctgcttgg
      721 tgtgccaggc ccggcccgtc aagccctggg tgtgtgtctc gtcttcgagc agcgagggca
      781 tgggctgctc gaccagcgac gtgagcatgg cgggctcgcc cgccatgaat tcgaactgga
      841 agatgccgtc ctggtcgggc ttgccgaagg cgcaggcggc gatttccgga tgctggctga
      901 gcagttcggc ggactggctg atcaggtcgg tgtagttgtc ggccgtccac accagctggc
      961 tcaggttgag caggacctcg tagcgcgcgg cctgcatcag ctggtaggcc tcggtctgcc
     1021 aggtgaggtc gcggatgagc cggcgtccca gcagccccag ggccttgcca tgccggttgg
     1081 catcgacgag gccgcgcagg gtggtgtgga ggatgtcctg tccgcgcaac aggtcttttt
     1141 gcgtcagtcc cacgatggcg tggatcttgc cgatgcgcag cgcggtggcg cgatggcgtt
     1201 cggcggacaa gtccggcgac gccagcaaca gcaggttctg ggcctgctgc gcttgcaagt
     1261 gctgcagccc ggccgtggtg agcatgccga tgatgtcgtc ggtatcggtc tcgccggtct
     1321 gctcgtagaa catcgcggcg atggccgaag ccttggggcg gagatcggcc tgcaaggcgg
     1381 ataggatgca agcccaggcc gggtcgtaca gctcgttgtc ctggaaggtg tcgatggcgc
     1441 cgtcggggcg caaaggctgg tcaggcatgg acggtgggca cgcaacaata cgtttttaat
     1501 acatttgtaa taaaaattac cggaggacta tagcactcag tccgtccgcc aatttgtcac
     1561 gcgcgccgct cccggttcga tatccgcgcg ttaccagacc gcgactgtcc ggctgcagga
     1621 tcgccattgt ttggtcaggg cgcgctcggc gcttttgccc tgcacggtgt agtccgtgtc
     1681 cgaagcgccg ccgctgtccg gatcgctgtt ccatcgccag accagcacgg tatggatggc
     1741 cggatcggac agctcggcca tccacgtggc cagcaccttg gcctgcagcg cctcgtcggc
     1801 gggggcgccg ttttcatcgg ggcgctggcc cggccgggcg gccgcgcccg atgcggagcg
     1861 tatgccgatc tcggcaatga agaggtgccg cttggcctgc ttggccagcg ccagcagatg
     1921 gtcgcgttcg ctggccatgg ccgcgcgcca gctgtcggga tcgttgtcgg cgcccagcgt
     1981 ggggtagagc gtggcgccca cggcatcgag cttgttccag aagggcaccc gttcggcgcc
     2041 ctcggcgccg cgcgccgcgt acacgagctt gccgtggaat atcctgcgca gccgcgcgat
     2101 gaggtccggc cattccggcc ggtccaggct ctggtcgagc tccgtgccga tcaccagggc
     2161 ttcggcatgc tcgtcttttg cgaccgcggc cagtccggca agcgcctgtt cgtaatgatg
     2221 gaaccactgg gcccagtcat cgtcgctggc catgcgcacg tcgcccggat cgccgtcggg
     2281 tatctggaca tgcggcttga cgatggcggc cagccccagc ttgtgcgccg tgcggatggc
     2341 gcgggccagc tcgccgtcgc tcatgtccga accgcgggac acatcggtgg agtcgctccg
     2401 gggttgccgg aagaagggca cgatggccac ggcattggcg ccggtgccgc gcagctgctt
     2461 gaggctggct tcggcctggg ctgaacccag cggggcgcgg tcatcctgga ccaggttgaa
     2521 gccatggcgt ggcggagcgc acggcgtggc ggcttgcgcg ctggcatcgg ccagcaggcc
     2581 ggcggctgcc agggccatgc atgcgccgcg gcgcaacagg gtggacaagc cgttgatgga
     2641 caaggggggg cgggcggaaa gcggcatggc gtatggcgat ggaggcatgg gcaccgtgcg
     2701 ggcgggggct ggaatcttca ttgtgcacct tgcttgctgc gatgcatgat tcgaaagcgg
     2761 gagaagtgtt acaacacgtc tgagggcctg ccttcaccgc gtgaaagccg acggcaggcg
     2821 agacagacga gcggagaccg acatgacaat agaagtccgt gccaaccagc ctgcaagcgc
     2881 cctgcgctac accgtaacgg ccgagggcca tggcatgccg ctgggctttc cccggcctga
     2941 tggcgacggc cccgatccgc atgactattt cgatggcgcg ctgggcggct gcaaggcgct
     3001 cacgctgatg tggtacgccc agcgcaagca gctgccgctg acctcggtgg acgtggatgt
     3061 cgtgcgtgac gccggcgagg aaaagcgcgg cgtctacaag ctgacggccc ggctcaggct
     3121 caacggcgac ctgagcgaag cgcagcgcgc cgagctgctg gccgtggccg agaaatgccc
     3181 catccaccgc ttgatggcga cggcggacgt gcagatcacg acggaactgg cctgatcggc
     3241 cttttgtccg gcacggcatg ccagttgcgc gttgcgccgg tccttaccct tccatgccgg
     3301 ggatgacggt cgagaagccc gcgtcgacgt gggtgatctc gccggtgatg ccggccgcca
     3361 ggtccgacag caggaaggcc gcggtgttgc ccacgtcctc ggtggtgatg ttgcggcgca
     3421 ggggcgcgtt ggcctcgacg aacttcagga tggacgagaa gtccttgatg ccgctggcgg
     3481 ccagggtctt gatggggccg gccgagatgc cgttggcgcg gatgcccttg ggacccagcg
     3541 tggtggccag gtagcgcacc gaggcttcca gcgaggcctt ggccaggccc atggtgttgt
     3601 agttggggac gaccttttcc gcgcccaggt aggtgagcgt gagcaccgag gccgggcggc
     3661 cctgcatcag gggcagcgcg gccttggcca tggcggggaa gctgtaggcc gaaatgtcat
     3721 gggcgatgcg gaaggcttcg cgcgacaggc cgtcgaggaa gtcgccggcg atggcttcgc
     3781 gcggggcgaa gccgatcgag tgcaccaggc cgtccaggcc gtcccaatgc ttgccgagct
     3841 cggcgaaagc gccttcgatc tgcgcgtctt cggccacgtc gcaaggcagc acgatgctgc
     3901 tgccgaattc ggcggcaaat tcgccgacac ggtccttgaa gcggtcgcct acataggtga
     3961 aggccagttc cgcgccctgc tggtggcagg cgcgggcgat gccgtaggcg atggatcggt
     4021 tcgaaagcac cccggtgacc aggatgcgct tgccggcgag aaagcccatg atttgtcctt
     4081 tttgtggtgg cgccagccca tggcgcggcg cgatgcggcg tattttaatg ccgcttttag
     4141 tgccgcttgg ccggatgctt cttgtccgcg ctggccacct tgacggcgcg gccgcccttg
     4201 gtgccttggg cgccgcgctt gctgccgccc cggccggcgg cctgggcgcg gcctttgtcg
     4261 gccagcttgc cgatggcctt ggcattgccc ttgcgggccg tgccgcgagt ctgccgggcg
     4321 cgggacgcat gatggtccgc gctttgtttg cggcctgtcg cggtcttggc atgggcgggc
     4381 ggcgggtcga ccggttcgga gtcgccgtcg gtcgcggcgg ccagggccag gggagcgcta
     4441 tcgcccacgt cgccggccgg caccagcagg ttctgcccgg agacggtctt ggcgtgcggc
     4501 gcgatgccgt tgagctggcg cagccggagc tcggagatgc ccaggtgcct ggccaccgag
     4561 gcgaccttct cgccgcgctg cggctggtag atccgccagt tgctgagccg gccctggtaa
     4621 ttggcgaagt tgtggttgaa gacctcgacc ttgtccttgg gcagcagcag ggtgctgttt
     4681 tcctggccgt tgatcaccgg gcgcttgtac gagggattga gcgccttgaa ctcgtcgagc
     4741 ggcatttcgg ccaggcgcgc ggcgatctcg aggtccatgt cgccgggctc gctgatcatg
     4801 gcgaaataag gcgtgttctc caccggcggc agggtgaccg cgtacttctc ggggttggag
     4861 atgatgttct tgatggcctg cagcttgggc acgtagttgc gcgtttcatc gggcatgtcc
     4921 agcgacaggt agtcggtggg caggcccagg ctctggttgc gggcgatggc gcgctgcacg
     4981 gcgttttcgc cccagttgta cgaggccagg gccaggtacc agtcgccctg catttcgaac
     5041 agctcggcca ggtagtcgag cgcggcgtag gtggaggcga tggggtcgcg gcgctcgtcg
     5101 cgccaccagt cctgcttgag gttgtagtgt tcgccggtgg ccggcacgaa ctgccacagg
     5161 cccgaggcct gggcgggcga ctgggccgtg gggttgtagg cgctttccac gaagggcagc
     5221 agggccagct cggtgggcag gccgcggcgg ttgacctcgt cgatgatgaa gtacaggtac
     5281 ttgcccgcgc gctgggccat gcgctcgacg gcctcggggt gggccgcgta atactccgtc
     5341 cattgctgcg acaggtcgtt gtcgaggttg ggaatggcga agccgcggcg gatgcggtcc
     5401 cacgcgtcgt gcgggggatg ggtgaggtcg atggtgcggg aggtgttgcg ggcgatgcgc
     5461 acgcccatgg cgctggtctg gtccgcctgg cgatcgatgc cggcgcagcc ggcaagcaag
     5521 acgagtagca ggaggagcag gtttcggatc aacgtcatgg gcttatttga attcgttctt
     5581 ccattgccgc agggcggcaa aaacattgac tggcgaggtc aaagggcggc ctgcccaggc
     5641 ggaagcggcc tggacgacca tggggtggcg ggtacgcaga aaaggattga tctgcaattc
     5701 ctgaccgatg ctcgaaggca gggtcggcag attgttttcg cgttgacgcc gggcctgttc
     5761 ttgcgccgct tgcgtggcgc ggttattggg ctcgactgcc aaggcccagt gcagattgga
     5821 caacgtgtat tcgtgagcgc aacaaacctg cgtgttgcct ggcaatgttg ccaattttgc
     5881 cagagattgg tccatttgtt cggcggtccc ttcaaaaagc cgtccgcaac ccgccatgaa
     5941 cagggtatcc ccgcagaaca gcacgggctt ttggccggcc tcctggccga ttcggccaaa
     6001 ataggcgatg tggccggccg tgtggccggg tacgtccagc accgccaggt ccaggccgag
     6061 ctcgggcagg acgacgtggt cgccctcggc caggcgcacg tcgcagcggg gcagcgcttc
     6121 tccggacggc ccgtataccg tggcccccgt cagccgggac aattccataa cgccgccgac
     6181 atggtcgcca tggtgatggg tgagtagaat ggcgcgcagt tccagccctc gcgcctgcag
     6241 gcattgcagc accgggccgg cttcgccggg gtcgaccacc gcggccaggc cgtcgcgaat
     6301 gacggtccag atatagttat cggtgaaggc aggcacgggc agcagctcgg cgccttgcga
     6361 ggacggaatg gaaggtttca tagcgaagac caatggctga gcaaatgctg ccgattgtag
     6421 aactggccga gtggttccag acgccgccgg gacaatacgt cctggcctgg gagcgcgcgc
     6481 agttcgacga gcgggtggcc gatgtgttcg gtttccaggc ctggcaggtg ggcttgcccg
     6541 gcctcgatct gctgcgggcc aaccgcatgc ctttcaaggc cgtggtgggc gccgagatcg
     6601 ccatcgacga cgctaccatc ggctggcgca acggcgtggc cgccctgccc gaggcgctgc
     6661 ccttcgactc gcagagcctc gatctgctgg tgctgccgca cgcgttcgag tgcaccgact
     6721 cgccgcacca ggtgctgcgc gaggtcgagc gcgtgctggt gccggaaggg cgcgtggtga
     6781 tctccggctt caatccctgg agcctctggg gggcgcgcaa cgccatgccg ggcatggagg
     6841 cctggctgcc ccggcccgtg tcgtcgcagg tgtcgccgct gcgcatgaag gactggctca
     6901 agctcatgtc cttcgaggtg gatgctacgc gcttcggctg ctatgtgccg ccctgccgta
     6961 ccgaccagtg gctgcggcgc tggcacttcc tggaagcccc ggggcagcgc tggtggaccc
     7021 tgggcggcgc ggtctatgtg atgtcggcca ccaagcgcgt gatcggcatg cgcatcatcg
     7081 gcccggcctg gaagaaaaag cgccagtccg ccagcacggc gtcggtggcc gtgcatcgca
     7141 gcgagctctg attcaccggg cttcgcgccc gttcattgtt gtagcaagga tattcatgca
     7201 gtcatcagaa attcaggacg ccgaccgggt ggaaatgtgg accgacggcg cctgcaaggg
     7261 caatcccggc ccgggcggct ggggcgtgct gatgctcgcg ggccggcacc ggaaggaaat
     7321 gcacggcggc gaggcgcaga ccaccaacaa ccgcatggag ctgctggccg tcatcgaagg
     7381 tctgcgtgtc ctcaagcgct cttgcacggt ggtcatccac accgattcgc aatatgtcat
     7441 gaagggcatg accgagtggc tcgccaactg gaagcggcgc ggctggatca ccgccgacaa
     7501 gaagccggtc aaaaacgccg agctctggca ggcgctggac gaacaggtgt cccgccacca
     7561 agtgtcgtgg cgctgggtca agggccacgc cggcgacccg ggcaacgagc gcgccgatgc
     7621 cctggccaac ctgggcgtgg acagcgtgcg ccagcgtgcc gggtaaacta gccggcaccg
     7681 ttttcattgc gcggaacacc acccatgcga cagatagtct tcgatactga aaccaccggc
     7741 ctgagacccg aggacggcca tcgcatcgtc gaaatcggct gcgtcgagct cgtcaaccgg
     7801 tccctcaccg gcaagaccct gcacttctac ctcaaccccg gccgcgacag cgaagagggc
     7861 gcgctggccg tgcacgggct gaccaccgag ttcctcgccg acaagccgcg cttcgccgac
     7921 gtggccgacg agttcctggc cttcatccag ggcgcggagc tgatcgcgca caacgccacc
     7981 ttcgacgtga agttcttcaa cgccgagttc aagctcatcg ggcgcggcga aatcgcgcag
     8041 tactgcgagt ccattaccga ctcgctcctg catgcgcgcg ccatgttccc cggcaagcgc
     8101 aattcgctgg atgccttgtg cgatcgcttc ggcatctcca acgcgcaccg gaccctgcac
     8161 ggcgccttgc tcgactcgga actgctggcc gaggtctggc tggccatgac gcgcgggcag
     8221 gacgcgctgc tgatcgatac cgacgatgcc gcgcccggcg cgggcgcggg caattcgtcc
     8281 ctgggggctt tcgatgcctc ggtgctgccg gtgatcgcgg cggcggccga cgagctggcc
     8341 gagcatgcgg cctatctgaa ggcgctggac aaggccagcg gcaagacggt gtggcgggct
     8401 tatgagccgc tggccgccga aggcgacgcc gcgtaaggac tgcgggcgcg gcgccacgtg
     8461 atccacagca gggcccccag tacgatgggg ccgagctgga tatgggtggg cgtcgcaaaa
     8521 ggcgccatga tcaagggcag cagccaggcc gcggcgagga tctcccgttc ggccgtgcgc
     8581 cacccggttt caagcccgca caaagccagc caggcaatgg gaaacgccaa ccaggccagg
     8641 tcgtagtcga agacataggg gctcaccagg aaagtgcccg tcatcaaggc ggcggcacgc
     8701 aggcgctggt cctggctgcc gcgccagatc cgccatacgg tgcggatcgc gaccagcgcc
     8761 acgacggcat gcacggcgta ggcaagagag acgggcgtgc ccagcaggcg caggaacgca
     8821 aagaccgtgg gcatcttggg ccagggcagc gtaccggttt caagaaaaag gcgggcatag
     8881 cccaggctgt gcacgctggc catcaaggta tccgtgccca tgatggccgt gctgatgacc
     8941 atgaacagga tcgccgtggc cgccgccacg acaaaggttc gccacgcgcc gatggcgatc
     9001 agggcaatgg ggaacagcag cgccaggtgc ggcttgatgg ccagcaggcc gatgaaaaca
     9061 cctgccagca cgggccgctt ttccaggcag agcagggcgg cagcggccag ggcggcggtg
     9121 agaaaggcgt tttgcccgtg gaagagattc atccaggcgc cggagaatgc ggccaggcac
     9181 cacatggcat ccggcgtgcg cacgatgcgc cgcatggcgc agacataggc cgccagcgtc
     9241 agcagcatga aggtccaata ggccggaaaa taagccagca aggccagcgg caggatcaag
     9301 acgtagaacg tcggcgggta gtaccagacg aagacactgt tcgacgcggg gacggcagct
     9361 tgttcggccc ggaagatcga agccaggtcg taggcgagcg gcgcatggtt gtgcaggccc
     9421 aggtatgaag ccgcccagaa agtgatgaag tcgtagccga gcggcttgcc cttgaggtcg
     9481 acatgattct tggcaagcag cgcccaggcg atgccgagca cgatgaacag cagcaggaag
     9541 aggcgcggat agcgcgtgaa gcgttcgcgg ttgagccagt gtgaggcggc atgtttcatg
     9601 cagtcagtcc tcgtatttca tgacgcgatt ttcttgaaca cgaaaacatg gttcaggcct
     9661 aattggaacg tcttgtgcga taccaaatta aggtgatcgg aaaaaatctg aaccaacgaa
     9721 gcagggtcga aaccgtgatg ttcttccacc gcttgtcctt ctatgagctt caagaacatc
     9781 aatacatcca ggattttgtc gacgatcggc gccgggaccg tgacgaccag tcttccggta
     9841 tcggcgagca tttgcgcaat cgcccgggcc gctccgatca agtcctgttc ggaaaaatgt
     9901 tcgagtaccg ccagggcaaa aacgacatcg tatttgcgat gcagtcctgc ctcttcccag
     9961 gctgagggaa aacaaccttg aagcagaacc gacccagcgg tcatgggttg cgcggcaagg
    10021 cgagggtcgc agccgtccag gatggcgctt cgattagcca cgcgcttgag cagatagcca
    10081 tcatcgcaac ctatatcaaa gacatggctc agctttttgg gtgcggctcg caacgctatg
    10141 tcggagcgcc atcggcgcag gagcttgtca agggccttca tggttttttt ccacgtctcg
    10201 tctgattggc aaggccagga agcccatgat caaggacatg agaaaaatga ttccacccag
    10261 tgtcatggat agcgtggaaa ggctaacggt tttgaggaag atgccctgtt gcagaacgcc
    10321 gaatcctgca gagctccaat ttttgagcag tctgaccccc aggtagccgc caaaaatcgc
    10381 cagcgcgatc ccgaggattc caccgatttc cagaatgggt tttgcgcgaa gtgtttcgaa
    10441 catccggttt ttcttgagca gcccttcgta catgccgaac atcctgacag ccagtccgag
    10501 catgatggcg atcagaccga cggtgatgcc agtttgcgcg taaaacaagg tattgatgtc
    10561 gaagacgaca ccgccgattt tcaactggcc cgcaaagatg gccaggtaga agaagatgct
    10621 gacgaacatg gtgatcaggc ctgggatcaa gaacagccac cggggactga aaagcagcat
    10681 gaaccggaag tggcgccatc cgtcccgcca gggacgcaag tgcggagcgc gtgtgcggcc
    10741 gtcctttgac aggcgcgttg gaacttcggc gatgcgcatg tcaagcaggc tcgccttgat
    10801 gatcatctcc gaggcgaatt ccattccggt ggtccgcaga tccatgcgca gaaatgcttg
    10861 tttgctgaat ccgcgcaaac cgcaatgcat gtccttgact gggcagttga acagcagccg
    10921 gcctatcctg gtcagcagtg gattgcctat gtatctgttt ttccatggca tcgcgccatg
    10981 agaaatccca cccaggaacc ggtttcccac gaccaagtcg tatccctgga ccagtttttc
    11041 cgtaaaaccg tccagggcgc caaaatcgta cgaaccatcc gagtcgccca tgatgatgta
    11101 ttcgccccgg gctgccatgg ctccctggta cagggcttcg ccatagccgc gcagtgcgac
    11161 gtcgacgacc ctggcgccat gcttacgcgc tatttcttgc gagccgtcat ggctaccgtt
    11221 atcggcgatg atgatttcgc cgagggctcc cgttctttgg agccagtcct gggctttttc
    11281 aatgcatgct gcgagcgttt ccgcttcgtt caagcatggc atcagaatcg agaattccat
    11341 gttttatttg tcaaccaatt cgtgtacggc gtcttgccag ggctctccga gttccttgac
    11401 ctcgcccgca tctttcagtt ggggggatga ggccagggct tgagcgagcc agggggcaat
    11461 gacaaaaacg cgatatccct tgttcaaggc ttgcgtcaag agcgtaccgt ccacttttcc
    11521 atggatggga tcggctacgg cgacaagcgt gtccgggtgg ccgatctggt gcattgctcc
    11581 ttccggcagg gccaggaaca gggagggcga tggcaacttg gcaaaagcct cgttcatcaa
    11641 cctgttctga tggcgatgcg cttgcgacac actcaagtcc atgatgttga atatccctgc
    11701 cgccatgaat ccgccaacgc cgaaggcgat ggccatccgt gcaaacaaga tgcgatgggg
    11761 gccttctcgg ccagcgagca ggcaaagcag accaagcgcg gcgaatagat aattggggaa
    11821 tatttgattt gcgccgtata tcgtcgctgg ccctgcaaaa gaccaggcta cgaacacaat
    11881 gatgaccgcc catcgtccca gtgttgcaag atgggtcgtc tttccccctt gcccggccac
    11941 cacgatgagg tcttcccaaa ccttggcggc caggatcgca gccaggggca gggccggcag
    12001 gaagtaacgc atattgctgc ctagcgcacc atgatagaga tgcaagatga agggcagggt
    12061 ccacagggta atacccatca gtatgaaagc gatgggttct cggtctgcgt ctttccagcg
    12121 cgagcagaca agaaaaaaca gcaagcccag ccagggcagg ctttgagccc acgctttttt
    12181 ggccatgccc cagaatgaca gggtgccgta tgcgcctttg accaccccgt catgaggatc
    12241 gatgatcgtg gtggcgtccc agatcaatcc ttgtgcgcca gtcatgtagt gagaaaggac
    12301 ttctcgggta gcggggattg ccaacgcaat gacgaagaga agccccaggc ttgccaggac
    12361 atggactttc cccagttttt ttgaacgatg cagccaatgc aatgcgatca gtgttgcgcc
    12421 caacaaaagc actagcaaga ttgcgccggt ctggcccgac agcgtagcgt tgtcaatcgc
    12481 tacggcaacg gtattcccgt aggagaacgg gttataggtg ccgaacttga attgattggt
    12541 cagcgatgtc gtgatgagtc caggcagaat gcctaccaga catccggcta gcagtatccc
    12601 ggcacggcgg ggggcaaata gcatggttgc caagcccgca atgggcaggc acaggattgc
    12661 atccagccgg aacaggagtc ccaggcccat gcatagaccc gacaaggtgg cccagcgaaa
    12721 attttggcta tcacgcgtaa tcgccaacca ggcgcacagc aacgtggctg ttgctccaaa
    12781 tacactgatg gcgtgaggcc atatccctac cgtgaattcc ggccaataag tggcaagcgc
    12841 cagaatgagc acggctcgcc aggcgatctc ttcgcggttg aacaagatgc gtgccaaggc
    12901 gcgtaccagg aacaatgtgc ctacggaggc cagcgcattg aaaaaaatca acgagcgagc
    12961 gcccagcagc ggcgccaata ccccgcctgc gatggccgtg ccgacaggat attgcggggt
    13021 caggccgtgc ggtccctcgg ccagcagcca aagcgtcagg gattcagccc tgaaggtgcc
    13081 gtacccattc tcgagtatca gggtatggtg atgctggaaa gcatcgacgc cggcctgata
    13141 gacgacttcg tcgacaatga acgcgccagt cgaggctacg caggctccga ttacgatggc
    13201 aaatgcaata agccatatgc cccagcgcat cgatgtgatc cgtatccgta taacggggaa
    13261 tttgtccgta tgggagtttg ccggaaattc ttacggtttt gttggcattt ttgcgcattt
    13321 tgtccggatt gctggacaat ctggcggcgg cgcggtgcct gggagacaag ccgtatagtc
    13381 ggctgtattt cagccatttg tatcccgatc cccgcgggta tacttgcgcg ccatgagatt
    13441 atccgcccct ccgttttcgg ctgactccgg ctggcagtac ctgatcgtgc tctgcgctat
    13501 tgccgtgccc agccttgccc tggtggtgcc tggcggcgcc aatatcgggt atttcgcctt
    13561 ggcggctgcc gtgtttgcca cgtgtttcgc tagcgggcgg gacgcgcagc gcatggcggc
    13621 cccggccggc gggatcgatc tgccggcgat tcttcccgtg agcctggcgc tgcccagcgt
    13681 ggcggtgttg tggacccagc ttgcccattg ggatttcgat ggacagccga tggacgcgcc
    13741 atcccgcttc ctgctggccg tcccgctgct gtattgcctg cagaggttgc cgccgtcccg
    13801 cttgcggcgg ctgcggcacg tgcagtgggg cctatgcgcc ggggcgatcg gcgccgcgct
    13861 ggtcttgtgg gtcgcgccgc tggacgggag catgcgccat gtgccgaagt ttgcttcggc
    13921 catcacgttc ggcaatctcg gcctggtcat gggcctgggg gcgatgctga ccctggggtg
    13981 gcgcctgacc cggtctttcc tggagccttt gctcaaggtc gtggccgtcc tggccgggtt
    14041 ctatgcctcg ctgttgtcgg gcacccgcgg cggctgggtc gcgttgccgg tgctgctgat
    14101 cctgttcctg ctgttgtcgc ggttgcgcgg cgcctacaaa ctgttgctgt gcgtgctggc
    14161 cgtggcgatc ctgtcggggg tgtacctgac ggacaagaat gtgcagatgc gtatccatac
    14221 ggccagcgcc gaatacgccg ccgcggtgca ggcgcacggc gaggagaaga gcgccctggc
    14281 tgcctcggac acgtcggtcg ggctgcgcct gtccttctgg cgcgcgggat acgccatgtt
    14341 cctggcgcat ccgttcgcgg gcgtggggtc gcagcatatt caggaggtct acgaggaccg
    14401 ggcggcgcac ggcctgatgc cgcaggatgc ggggcacttc gaccacctgc acaacgacta
    14461 tctccagtcg ctggcggcca atggcttgcc gggcctgctg gcgcggctga tggcctacct
    14521 ggtgccgctt tacctgttcg tgcgcgccat acgctcggcc gagccgcaac gggtgacggc
    14581 gggctgggtc ggggccctcg tggtcgtgag ctatatggtc ttcggcctga ccgagaccat
    14641 gttcagcatc accatgaatg ccagtttcta tactggactg gtgctgattt tttcggttct
    14701 ggccttgccc gccaggcctg gaacgacagg ctggcaggcc tagccggccc attgcggctt
    14761 gccaggagcg attgggcgtg ctagaatctt gcttctttgt tgggtggtta gctcagtggt
    14821 agagcactgc cttcacacgg caggggtcac aagttcgaaa cttgtaccac ccaccaaaaa
    14881 ctcccacaag aagatcaagc actcaggcta agttggccgg ctgtgatctt ctgtctcccc
    14941 ccgcgcgcac tccccccgat ctgtctgtcc tactcctgtc cttcgaagac gagagtccgg
    15001 ccgcattgcg caagcgcaac ggcaagtgga acgcatgcat ccagcgcagg gattaccccc
    15061 aattatccaa gacttttgcc gcccaggctg atgctaaggc ctgggcgcat tggtccagtg
    15121 ccgtacatta gggctggcag actgtcctga agcgtcaatc gattgatgaa gcaagccaaa
    15181 gtccagggca tgatccatcg cggcattggc ggctgcggca ggtctgagca catcctgcgc
    15241 atcgaccacg cgatcacgtc cttgcgtttt ggccgtgtag agcgccgcat ccgcgcgtgc
    15301 cagcaactcc ggcagcgagt cgctgtaccg atactcgtcg atccccgcgc agaacgagta
    15361 gcgatgcggc ggtatatctt ttgcatcgtt cagcatcccg cgccggtcga aattcgtccg
    15421 ctcctgcagc acttccatgc gcgaccaggc atcctgccgg ccggttctcg agaaaaggat
    15481 tgcaaactcc tcgccgccca cccggccgaa cacatcggtc tcgcgcagag tcgtcctgac
    15541 cagccggccg aagtgcgcga gcacactgtc gcccgccgca tgtccgtatt gatcgttgat
    15601 caacttgaag tggtccatgt cgatgatcgc aaccgatagc ctcgcgccgg aagcggttgc
    15661 atgctcgagc atctcttgcg ccttcgcgaa aaatcggcga cgggtaagcg cccccgtcag
    15721 atcgtccacg ttcgcccagc gctccaactg ttcggccagt cgatcatgca cgagtatcac
    15781 catgcccatg gacaaccccg gcagcacaag catgccgacc atgggaaacg caatactgga
    15841 tacggctgat tgaagcagac tgccttgcgg cagcaagcct acgccgtaga cgagtccgcg
    15901 gatcacatga cccagcgcac tggcgcaagc gatcaccacg atgaagcagc agccgtagga
    15961 aggccgcttg ggcgcccgat gcttccaggc cgtccagccg atactggcgt gcatacaggc
    16021 atagaaaatg gacactgtag caatacaggc gcccatattc ggcgatgcgt aagtccaata
    16081 ggcaatgccc agcacaaggc cggtgcagcc gacgtacggc agcatggtga caggctggag
    16141 gtggaaaaat tgccggcaac cctgataccc cagcacgctg gcgaaggcca gcagcaagtt
    16201 ggccacgagc accgacagga tggtcgggct gtcatgctgc agcgcaaaca gtatcaaggc
    16261 aatgatggcc gctgcgttcg cgcctatcca gcgcgagacg cccgggacgg tcgaatgccg
    16321 cagcgaaccg gaaatcgcca tgctcatgat ggtgacgagc atcatggcgg ctatcagaac
    16381 actgactgga ttcagcatgg ctgccacggg cacggccggc ttgggttccc ctgcatggcg
    16441 agactccctc ttatctgtgt gttggagcgt gcgtcgagat aaaaccatcc cggcatacat
    16501 catgtcgcta gtcactttga caccgatcag aggccttatc ggttcggtgt cgaacaatac
    16561 gaagcacgag cagataagag aaaaacccgc aagaatcgcg cttgcgggtt tttgttccgg
    16621 gtttttgctt tgggcgctga agagacagcg ccatctcagt cagtccatca cggcatgggc
    16681 cgctgcgccg ctgcctgcgc tgcggcggcg ccggatgaat atcaccaggg gcaggatgca
    16741 tagcgtggcg atgaacatca gcttgaaggc atccaggtag gcaatcattg aagcctgctg
    16801 gtcgatgatg cggttgacgc tttcacggcc ggtttcggtg gccaggtcga agtattgctg
    16861 ccagccgctt tcgccgcggc tcacgtgctc ggccagcgag gcgtgcacgg tctggctgtt
    16921 gcgggtgacc acggtttgca cgatggagat gccgatgctg ctgccgatgt tgcgcattag
    16981 gctgaaaatg gcggtgccgt aggcgcgcag ctcgggcttg agcgtggcga agctgacggc
    17041 cgacagcggc acgaacacca ggccgagtcc cacgccctgc accacgccgg gccagacgat
    17101 gtcgctttcc gacagcgtca gggtgtagcc catcatctgg tagagcgcgt agcccgagat
    17161 cagcaggccg gcgatgagca tgccgcgcac gtccaggcgg cccaccaggc ggccggcaat
    17221 gagcatggcg atcatggtgc ccagaccgct gggcgcggtg accaggccgg tggtggccac
    17281 ggggtagtcc atcaggtttt gaagcatggg cggcagcagg gcgcgcgtgg cgtaaaggat
    17341 ggcgccgatc acgaagatga agaggctgcc ggtgacgaag ttgcggtcct tgagcagctc
    17401 ggcgcggaag aacgatctct tgcccgcggt ggcggtatgg atgaggaaga aggtgaagct
    17461 gacgaaggcc acgatggctt cgatgcagat ctccgtcgaa ttgaaccaat ctttttgctg
    17521 gcccctgtcg agcatggctt gcaaggcgcc gatggacagc gacagcgtgg tgaagccgaa
    17581 gacatccagc gcgtggcgtt cgccctggcg gatcttgggc atgtaggtca tgacgcccag
    17641 cagggccagg gcgccgatgg gcaggttgat gaagaacacc cagcgccagt tgtagctgtc
    17701 ggtcagccag ccgcccagcg tggggccgag gatgggtccc accatcacgc ccacgcccca
    17761 gatcgccatg gcctgcggct gtttttcggg cgggttgatg ttgagcagga tggactgcga
    17821 cagcggcacc agcgcggcgc cgaaaaggcc ctgcagcagc cgtgccgtga cgatctggct
    17881 caatgaatcc gaaatgccgc acagcgcgga ggccacggtg aagccggcga tcgacgcgca
    17941 cagcagctgg gtggtgccgt agcgcgccgc cagccagccg gacaacgggg tggcgatggc
    18001 cgccgccacg atataggagg tgagcaccca gctgatttcg tcttgcgagg ccgacagcgc
    18061 gccctgcata tgcggcaggg caacattggc gatggtgctg tccagggtct ggattagggt
    18121 tgccagcatg atcgacaggg tgatcatgcc gcggttcaat ggggcggaag tgtcggcggg
    18181 gggcatggag gctcgttacg tatgccggct tataataagc tagcttatta atttactcaa
    18241 agagttcggc gcaattccgg ggatggtcca tgtccaacac ctatgaagaa cgttttggtt
    18301 ttctggtctc ggatatcgcc cggctgctgg ccacccagtt cgaccggcgc gggcgccagc
    18361 ggctggtgct gagccgggcc cagtgccggg tggcgctata cctctccacc ctgggcccca
    18421 tgagccaggc caagctggcc gaaacgctgg acgtcacccc catgaccgtg gcccgcatgc
    18481 tcgaccgcat ggaagaggcg ggctgggtgc agcgggtgca ggcgcccaac gaccgccggt
    18541 ccttcctggt gcgggtgacc gccaaggtcg aggaggcgat ggggcctgtc ctggacctgg
    18601 gcgacgagat taccgccttg gccatgcagg gcctgagcga cgaggaaagg cagaccctgc
    18661 tgcggctgct gagcaaggtg cgcggcaacc ttggctcgag ccaggccgaa ggctgagcgc
    18721 ggctttcgtg ttgccgcagg caaacaaaga taaacgtggc gagaacccgg cttgcctgtc
    18781 cggccgttct cgggcagcat cgctgcatgc cgggcgctgg acgatcgatc gtcgaacccg
    18841 gcttcgtccg tccacttcct ctcgataccc ctcccgccat catgataggc cgcgacttcc
    18901 tgcattcccc gggccccacc cacatccctc agccggttct caatgccatg aaccggcaac
    18961 ccctggatct cgtcgacgac cggctcaagc tgctcgcgaa atcctgcttc gacgacctga
    19021 aggatatttt ccgcaccaag ggccaggtgt tcatctacgc gggcaacgga cacggcggct
    19081 gggaggcggt gctggccaat atctgcgcgc ccggggacac ggtcctggtg ccggtcgtcg
    19141 gccattttgc ccatgaatgg gcggaccacg cccgcgcgct cggcatcaag gcggaaacca
    19201 tcgagggcga ctggcgccag ggcatcgacc cggcgcagat cgaacagcac ctgcgccgcg
    19261 acaccgagcg ccgcatcaag gccgtgctgg ccgtgcaggt cgataccgcc gccagcctga
    19321 gctgcgatat ccaggcggtg cgccaggcca tggacgcggc gggccatccc gcgctgctgg
    19381 tcgccgacac cgtggcctcg ctggcggcca tgcccttcga catggatgcc tggggggtgg
    19441 atgtcgccat cacggcgtcg caaaaggccc tgatgtgccc gcccggcctg gccatcgtgg
    19501 cggccaacga caaggccatc gaggccgcgc gcagcacgcc ccggttccag cgctattggg
    19561 attggatgca gcgcatcggc gacgagtcct acaccaagtt ctgcggcacc atgcccgagc
    19621 accacatgtt cgcgctgcgc acggcattgg acctcatcaa gcagcgcggc ctggagacga
    19681 tcttccgggc ccagcgacag ttgtccggcg cggtgcatca ggccgtggag gcctggagcc
    19741 aggcgggcgc gatggcgttc aacgtgcccg cctcctccga gcgggccgtg accgtgacga
    19801 cgatacgcac gtcggccggc atcgacccgg accgcctgcg cacctatgcg cgcgagaaac
    19861 tggggctgat gatcgccggc ggcctgggca agctgggcgg ctcggcattc cgcatcggcc
    19921 atatgggcag catcaacgaa ccgatgctcc tgggctgcct ggccacgctg gacctggcgc
    19981 tcaaggagct gggactgccc cacggcgagg gcgccgtgca ggcggccatc gcccacctgg
    20041 cacgcgagcg cacggcggct gcctgacgcg gcatggcgcg ggacgccttt gcggcgcccc
    20101 gccgttcggc attcaagggg cgggtttgca gccggtcgtc ggcggcacga tgccccaatc
    20161 caccgttact tgcgggccgt tggcgttgcg cgtggcaggc aggcgcgcga tgccctgcca
    20221 ttgggcctgc ttcattcttt cggccaccag gggccagacc gggtcgtgat tgaacgggct
    20281 ggcgtcgatg acgaccagca tgccgcaagc cgcgagttgc ccggggtcga tccagggtgt
    20341 gttctgcgga tcgccgtcga tcaggacctg cgcctggcgg cccgcgtgga tggcgatgtt
    20401 gccgcctatc caggtgtcgg ccgcgatgaa gcgcaggggg ccgtcgacat ggtcgtgcca
    20461 gacctgctgc aactgccggg agacctggct gctggggaaa ttcgagcgtg tcggccggcc
    20521 catcaggctg ggcagggggc cgcgccccgc ggcatagccg accacggtga gcacctgcat
    20581 cacgccgacc acggccaggc tggtcttgag caggcgccgg ccgccgtgtt ccgcatacag
    20641 ccagaacgtc aggaggcccg ccatgaggaa gaaggtggtg gtccagtcgg cgatcatcca
    20701 cgtgtcggcg atgaaggagg ccgcgcacgt cagcacgaag gggccgaggc ccagcatcag
    20761 gatgaagcgg cgatcctgcg ggcgcagttg cgcggcgatg ctgccgggtt tgccgccggt
    20821 gccgccggct tcgccagcgg gccgccggcg cgcggcgtgg cgcagcaggc cgccgatgat
    20881 cagcatggcc agcagcatgg gcgcgatgcg cgccagcgtg gtggtgaaca catagtagat
    20941 ggccagcagg ttgctcgcgc gggtcatgga gtccgtgcgc gtcgccacgg tggcgtaggt
    21001 cagcggattg gccggattgt gcagcacgta ttccatgttg tagagccaca tggcgtgcgg
    21061 cagcagcatc acggccagca cgcaggcgcc cgccagcacg cctttccatg tgcgcagttc
    21121 gcgcaggctg ccgcttgcca gcagttgcag ggcgaacatc acgaaccaca cgcccgcgct
    21181 gtacttggtg agcatggcaa gggccgagaa cacgcccagc agcagccagt cgcgcaactg
    21241 ctggtagcgc caggcgcggt agaacatcca gatcgcgccg gccaccgacc agagctggat
    21301 ggtgttgtgg ttggacatca ccccgcgcgt cgtgaaatag gcgatgggca tcatcagcag
    21361 cacggccagc agcgcattgc gctgcgaggt catttcgcag cccagccgcc agatgaagta
    21421 gagcgccagc gtgacgctga gctggccggc gaagaaggtg atccagactt ggcggccgaa
    21481 cagggtgatc agcccgtagt gcacccagga cggcaggggc gggtgcttgt agtagcccca
    21541 ctggaacaca ttgccccaga tcagctcttc catattgtcg acatccggcg cgctgtggct
    21601 caggccgagc agcacggtcc acagcaggag ctgcaggccc aggagcagca ggaccagggg
    21661 ctggagcgag acggcgtcga gggagaatct ggcggccagc gcgtcccgcc atgatgcgct
    21721 gctgggtgcc gcagacgagg ggggcgctag ggattgcgaa aggccggaat ttgcccgcat
    21781 gctgagacac accaacagag gaggccgcta cctggccgag ggtcgtaagg gtgacggatt
    21841 ttattgtaat ttcaagcgac tgcggccggc ctcggtttgg ctggccgtct gcccgatgga
    21901 tttacgcggt aaccttaagg gccggggcgg cggccgggtc gcggcccgcc tggcgcaagg
    21961 ccccgggatc cgtgtcgatt cggccacaat tcgacgccgt tggtgcatac ttcgcacctt
    22021 tatctgtttt ttgcggtgaa accttgacca catcccctct cgatcagagc gttgccgtac
    22081 acgccgccga gtcggatccg cttgcgccgc cccggtcggt cgacggccgg cctcccgtgc
    22141 gcggcctggt gacctgtgtc gttcccggcc tgaacgagtc tgaaaacctc aaggtgctgc
    22201 tgcctcggct cagcgctctg ctggagcagt ggtgcgacgg ctgggagatc ctcgtggtcg
    22261 acgacggcag caccgacgac acgcccgcgc tcatggccga atggaccaag accccgggcg
    22321 tgcgctatgt gcagctgtcg cgcaatttcg gcaaggaagc ggcgctggcc gccgggctgg
    22381 aagcgtccgc gggcgatgtg gtgatctgcc tggatgccga tctgcagcac ccgctggagc
    22441 tggtgccccg gatgctgcag cgctggcagg cggggatcga tatggtctat gccgtgcgca
    22501 gcgaccgcgt cgacgaaacc tgggtcaagc gcgtcggcac ctggttcttc taccgcctgc
    22561 tgggcagcac gcgccgcatg cgggtgccgc cgcatgcggg c
//
LOCUS       DASWPO010000334        11491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2136275, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000334 DASWPO010000000
VERSION     DASWPO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136275"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="VGC69_17740"
     CDS             <1..1645
                     /locus_tag="VGC69_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY0297176.1"
                     /translation="TWSLFGNFPAILDDKVVGDLFGEGKMFLPQVVKSARVMKQAVAH
                     LIPYIEEEKRQIAAAGGDVRAKGKMVIATVKGDVHDIGKNIVSVVMQCNNFEVVNMGV
                     MVPCAQILDMARAENADIVGLSGLITPSLEEMAYVASEMQRDDYFRSRKIPLMIGGAT
                     TSRVHTAVKIAPHYDGPVVYTPDASRAVAVAANLVSDQAEAYIAEIAQEYEEVRRRHA
                     NRKAAPLVPLEAARAARPAIDWSGYTPPRPKFIGRRTFKSYDLADIAKYIDWGPFFNT
                     WSLFGNFPAILDDKVVGEEARKVYADGQAMLKKIVEGRWLKANGVVGFYPANTVNDED
                     IEVYKDESRGEVLFTWRNLRQQGAKREGVSNKSLADFIAPKDSGVEDYIGLFAVTGGL
                     GLEHKEAEFLAAHDDYSAIMLKSLADRLAEAFAECLHARVRQDLWGYATDEALSRDDL
                     IAEKYRGIRPAPGYPACPEHVVKTEMFHVLDAQEIGMYITESYAMLPASSVSGFYFSH
                     PESQYFNVGTIGEDQLADYVRRSGRGEDDVRRTLAPNLG"
     gene            complement(1664..2614)
                     /locus_tag="VGC69_17745"
     CDS             complement(1664..2614)
                     /locus_tag="VGC69_17745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0297177.1"
                     /translation="MRLLRIFRAVAQAGGLAAAERELDMDRSTISRSLSTLETRLNAR
                     LCFRGPSGFELTDFGRATLRAAINACDTLEMISHELNLARSVMSGDLYIGIADNCLTN
                     PRCALVQALAQFRRLAPNVTVHLSIHMPSELLARLADRHLQLGITGTALGVVGTMLGN
                     ERLRCQPLFTETFRLYVAARDPLPAPCLDELATEGYELVVRDRDPHTRKLVERVGIAR
                     HGISTGLEAVATLLASGHFAGFLPTHYVDALGSAYRYAPVRDAEELAYETIFSLVLVE
                     DRIISPSTELFMQLLASTHETGAAAPRGSALASQGVPATA"
     gene            2916..3881
                     /locus_tag="VGC69_17750"
     CDS             2916..3881
                     /locus_tag="VGC69_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY0297178.1"
                     /translation="MTLPLTVPPFAGHQSFLYSPIQTDLDKLDAHVAIMGLPFGQAYD
                     ATRITNDQTLAPDAIRSVTTRVSRGLERYDYDVGGPIYDGRALKVVDVGNVPADMNDL
                     RSHYARAEQAIRKILAAGAMPITLGGDHGITTPVLRGFADEGPITVVHVDAHLDWRDH
                     VNGVREGLSSPIRRASEMKHVKAIWQIGMRSQGSARLEEAQAAAAYGSNIVPAHEVHD
                     AGMDAVLERIPDGGRYYITIDADGLDPTVMPAVEGPAPGGLTFHQVRKLIHGLVRKGR
                     VVGMDIVEITPSIDVNMITCITAGRLMVNLIGAAVRADYFDAPRL"
     gene            3942..4802
                     /locus_tag="VGC69_17755"
     CDS             3942..4802
                     /locus_tag="VGC69_17755"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0297179.1"
                     /translation="MKRITCTARVMFAVGLAAIMLLASAGASAETTLERIRRTGELTV
                     GTEAAYPPFEFVKDGKIVGYGSDILAEVAKELGVKQFNQLDLPWQGILPGVLAGKFDF
                     VATTVGINEERAKRYAYTMPIADGAPYFLQRKDDGAKLETGLNGKVVATQLASSTEPI
                     ARQLDAQLKAAGGSGFKELKLYTSFPETYVALANGEVDWVLQGLPSLSVVVKEHPDTF
                     KLGGPVPVTGLYTYLAWVARPEDKDLRDFISGVIRKMRDDGRLGALQKKWFGFEMKIP
                     DSGYLPPGAV"
     gene            4825..5481
                     /locus_tag="VGC69_17760"
     CDS             4825..5481
                     /locus_tag="VGC69_17760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0297180.1"
                     /translation="MKFNFHYLLSVLPPLGSAALVNLRLAAIAIVLALVLGTALTVLR
                     SRKIGPVNAAIAVLISFVRGTPLLIQIFIFFYVLPAVGLNLSAPVAGVAALACNSMVF
                     VSETLRAGLGTLDPGQIEAATALGLPPRVIWIKVILPQVLRRSLPMLANEASIVIKAT
                     ALLSVITVVDVLRTAQQIAGASFRPFETLFGAALMFALINMLLSLCTRMVEKRVMLRV
                     "
     gene            5489..6211
                     /locus_tag="VGC69_17765"
     CDS             5489..6211
                     /locus_tag="VGC69_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0297181.1"
                     /translation="MRFEPSIILDNAPLFLKGALLTLEISACALSIGFVLGVAIALLR
                     LAPSRVLHGFAGGYIAVLRSVPFIIFLFLVYYGLPFSGIRIPALTTGIVSVALFSSPY
                     YAEVIRSAIQALPAGQYESARVIGMSPAQAMRHVIAPQIVPALIAPATNVTLTMIKDS
                     AVLSSITIPELSYQGLIVQGNTFAPFEVFAAVAGLYWLISMAIAEFSRRLELRVAGMR
                     ERRATRNALADRYLSLDKRTAA"
     gene            6208..7023
                     /locus_tag="VGC69_17770"
     CDS             6208..7023
                     /locus_tag="VGC69_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0297182.1"
                     /translation="MSPSKPALRLSGIVKNFGPTTALNGLYLDVHEGEIVALLGPSGC
                     GKSTLLRCATWLEQPDDGFIEVAGKPFGRMRTTGGVVRHQTREEIDRIRPRIGLVFQQ
                     FNLWPHLTALDNIVCAQCVVLGRERDAARRRGLELLDRLGLATLAHRRPAELSGGQRQ
                     RVAIARALAMDPAVMLFDEPTSALDPELVGEVLSLLKLLAGQGTTMLVVTHSVSFAAA
                     LADRIVFMDAGCIVEEGPPTSLLKAPRSERLRVFLEHTLSERPAWPPVVAQEA"
     gene            7027..7992
                     /locus_tag="VGC69_17775"
     CDS             7027..7992
                     /locus_tag="VGC69_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY0297183.1"
                     /translation="MQRVNRPFVGIPSFLRSRVCEDLTRLDADIAVFGVPFDEGSPYL
                     PGTRMGPRALREHSMRFCGGSGGYYNPQTRGQYLVEEMNLGMIADVGDVDVHPTNPER
                     TFQNVTETVRAILDRGALPIGLGGDHSITYPIFRAFDEPLHVLHFDAHTDFAPFEKDL
                     RLTNGHAFRHIAAMPNTLSLTQVGIRSLRHSRAQIDDVKTSGNRVVPINDFREMGPEG
                     IAALLPEGSRVYVTVDVDVLDMSLIPGCVSAEPDGMSYADLRDSLKAIAARHDVAGFD
                     FVEVNPLLDVATGITSYLGALVVVEFLGNICAQPRWAARRLARAR"
     gene            8216..9052
                     /locus_tag="VGC69_17780"
     CDS             8216..9052
                     /locus_tag="VGC69_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0297184.1"
                     /translation="MPQLAQLPSDAYTKRRQVLGEFVRNARARITPQMAGLPEGARRR
                     TPGLRREEVAQLSGISVTWYTWIEQGRDVSVSPTVWARIASVLHLGKAERAYLFELAE
                     CADPQQPREAPEGVAERLKACVDAISAPAYVLDHAWNVLAFNEAMRDLFDDWPAREPR
                     PNLLRYIFLDPAARRLVVDWEQRARRVVAEFRADAAPNLDVPDVAALVDALDRESPMF
                     AHWWTRHAVIEREGGFRDFDHPRRGRLAFQQITFRLATHPELKLVMLLQPEYARALAI
                     PD"
     gene            complement(9049..9336)
                     /locus_tag="VGC69_17785"
     CDS             complement(9049..9336)
                     /locus_tag="VGC69_17785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY0297185.1"
                     /translation="MRMHNPPHPGSILKDDILPELGLSVTEAARQLGVSRVQFSRFIN
                     GRSSVTPELAVRLSKWIPAPTAIMWLQMQIEHDLWQLEHSGKRLDVEPAHA"
     gene            complement(9611..10651)
                     /locus_tag="VGC69_17790"
     CDS             complement(9611..10651)
                     /locus_tag="VGC69_17790"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY0297186.1"
                     /translation="MRDETVRLPARQNGRDIQLTATLYTPNGEGPFPLVVINHGKSPG
                     KASEQPDSRFYPESLQFVRRGYAVLVPVRAGFGSSGGSYLSGCRQPLATLSDFWADDV
                     KAAIDYARTLPYIDTRRIVVVGASQGGLVSTALGVRDVPGLLGIVNFAGGLRQENCRD
                     WANRNTAAFRTYGANTNVPALFIYGDNDAYWGDGKLSRRFFNAYHAGNPRSTYVDTGV
                     FAKDTSHHLFENFDGLDIWVPIVGRFFQRLGLTWDIVQPPHSDGAAALGDADAVPFQR
                     SRNTIRSGYERFLLMDPKNGRAFALSENGAWSRQSGKNAAQKALDSCREHRGVDCRLY
                     AVDGQVVYQNSP"
     gene            complement(10821..>11491)
                     /locus_tag="VGC69_17795"
     CDS             complement(10821..>11491)
                     /locus_tag="VGC69_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0297187.1"
                     /translation="LDGNVKRVFTRHFGIEGDPAKRAVDQQLWQLADAQVAAAPALDM
                     PAYTQGLMDLGATLCTRGKPACGRCPVAGTCVARRDGRQAELPTPKARKTVPERETAM
                     LILARGGAVLLQQRPSPGIWGGLWSLPEFEAGGDPRAAARALGLEPAEHYRLAGFTHV
                     FTHYKLHVQPWYVTVSGQAALREAGLPEQWLAVGALPSAALPAPVKKLLEGLAAAGLP
                     DASP"
BASE COUNT         1917 a         3638 c         3920 g         2016 t
ORIGIN      
        1 tacctggagc ctgttcggca acttcccggc catcctggac gacaaggtgg tgggcgacct
       61 gttcggcgag ggcaagatgt tcctgccgca ggtggtgaaa tcggcgcgcg tgatgaagca
      121 ggccgtagcc cacctgattc cctacatcga ggaagaaaag cgccagatcg cggcggccgg
      181 cggcgacgtg cgcgccaagg gcaagatggt catcgcgacg gtgaagggcg acgtgcacga
      241 tatcggcaag aacatcgtct cggtggtcat gcagtgcaat aacttcgagg tggtgaacat
      301 gggcgtgatg gtgccctgcg cgcagatcct cgacatggcc cgcgccgaga atgccgacat
      361 cgtcggcctg tccgggctga tcacgcccag cctggaggaa atggcctacg tcgcgtccga
      421 aatgcagcgc gacgactatt tccgcagccg caagatcccg ctgatgatag gcggcgccac
      481 caccagccgc gtgcacaccg cggtgaagat cgcgccccac tacgacggcc cggtggtcta
      541 cacgcccgac gccagccgcg cggtggccgt ggccgccaac ctggtgtccg accaggccga
      601 ggcctatatc gccgagatcg cgcaggagta cgaggaagtg cgccgccgcc atgccaaccg
      661 caaggccgcg ccgctggtgc cgctggaagc ggcgcgtgcc gccaggcccg ccatcgactg
      721 gtcgggctac acgccgccgc ggcccaagtt catcggccgg cgcaccttca agagctacga
      781 cctggccgac atcgccaagt acatcgactg gggcccgttc ttcaatacct ggagcctgtt
      841 cggcaacttc ccggccatcc tggacgacaa ggtggtgggc gaggaggcgc gcaaggtgta
      901 cgccgacggc caggccatgc tcaagaagat cgtcgagggc cgctggctca aggccaacgg
      961 cgtggtgggc ttctacccgg ccaatacggt caacgacgag gacatcgaag tctacaagga
     1021 cgagtcgcgc ggcgaagtgc tctttacctg gcgcaacctg cgccagcagg gcgccaagcg
     1081 cgagggcgtg agcaacaaga gcctggccga ctttatcgcg cccaaggaca gcggggtgga
     1141 ggactacatc ggcctgttcg ccgtgacggg cgggctgggc ctcgagcaca aggaggccga
     1201 gttcctcgcg gcgcatgacg attattcggc catcatgctc aagtccctgg ccgaccgcct
     1261 ggccgaggcc ttcgccgagt gcctgcacgc ccgcgtgcgg caggacctgt ggggctatgc
     1321 gaccgacgag gccctgagcc gcgacgacct gatcgccgag aagtatcgcg gcatccgtcc
     1381 cgcgccgggc tatccggcct gcccggaaca cgtggtcaag acggagatgt tccacgtgct
     1441 cgatgcgcag gagatcggca tgtacatcac cgaaagctac gccatgctgc cggcttccag
     1501 cgtgtcgggc ttctacttca gccaccccga gtcgcagtac ttcaacgtgg ggacgatcgg
     1561 cgaggaccag ttggccgact atgtgcggcg cagcgggcgc ggcgaggacg acgtgcggcg
     1621 caccttggcg ccgaacctgg ggtaggggcg gcgttgcggc cggtcaggcg gtggcgggca
     1681 cgccctggct ggccagggcg ctgccgcgcg gggcggcggc gccggtctcg tgggtgctgg
     1741 cgagcagttg catgaagagc tcggtgcttg gagagatgat ccggtcttcc acgagcacca
     1801 gcgagaagat ggtctcgtag gcgagttctt ccgcgtccct gacgggcgca tagcggtacg
     1861 cggaacccag ggcatcgaca tagtgcgtcg gcaggaagcc ggcgaaatgg ccgctggcca
     1921 gcagcgtggc cacggcttcc aggccggtgg aaatgccgtg gcgggcaatg ccgacgcgtt
     1981 cgaccagttt gcgcgtgtgc gggtcccggt cgcgaacgac gagctcgtag ccttccgtgg
     2041 ccagttcgtc cagacagggc gcaggcaatg gatcgcgcgc cgcgacatag agcctgaagg
     2101 tttccgtaaa gagcggctgg cagcgcaagc gttcgttgcc cagcatggtg ccgaccacgc
     2161 ccagggccgt gcctgtgatg ccgagttgca gatgccggtc ggccagccgg gcgagcagct
     2221 cggaaggcat gtggatcgag agatgcacgg tcacattcgg cgccaggcgg cggaactgcg
     2281 ccagcgcctg cacgagcgcg cagcggggat tggtcaggca gttgtcggca atgccgatat
     2341 acaggtcgcc gctcatcacg ctgcgcgcca ggttcagttc atggctgatc atctccaggg
     2401 tgtcgcaggc attgatggcc gcgcgcagcg tggcgcggcc gaaatccgtc agctcgaaac
     2461 cgctgggccc gcgaaagcag aggcgcgcgt tcagccgggt ctccagcgtg ctcaggctgc
     2521 ggctgatggt ggagcggtcc atgtccagct cgcgttcggc ggcggccagc ccgccggctt
     2581 gcgcgacggc acggaagatg cgcaacaaac gcaaatcatt ctcatcgatg ggtgcgaggg
     2641 tttgcccttg gctgctcgcc atgcgtgcat ggccatgagc aatcgtgggg cggcttgcac
     2701 tgcgccggtg cgtaagactg tgctcttcca tggaattccc cagaacgagt ccgcatgcag
     2761 aaaagagtcg agcaaaaaat gtgccaatag aaggccggcg aaggtttcgg cgcgcgcatg
     2821 catggttgcc gaaacgcccc tccttatcgt gcgcacgcct gcctagactg agccgggctt
     2881 gatgcaccgt ttcgatagac gaggagggaa taggtatgac gctgccattg accgtgccgc
     2941 cttttgccgg ccaccagagc tttctgtatt cacccattca aaccgatctc gacaagctcg
     3001 acgcgcacgt cgcgatcatg gggctgccgt tcggccaggc ctacgatgcc acgcgcatca
     3061 ccaacgacca gacgcttgcg cccgatgcga tccgatcggt gacgacgcgc gtctcgcgcg
     3121 gcctggaacg ctacgactac gacgtgggcg gtccgattta cgacggccgc gcattgaagg
     3181 tggtcgacgt gggcaacgtg cccgccgaca tgaacgactt gcgcagccat tacgcgcggg
     3241 ccgaacaggc catacgaaaa atactggccg ccggcgcgat gcccatcacg ctgggcggcg
     3301 accatggcat caccacgcca gtgctgcgcg gcttcgccga cgaagggccc atcacggtgg
     3361 tgcatgtcga tgcgcacctg gattggcgcg accacgtcaa cggcgtgcgc gagggcctgt
     3421 ccagccccat ccggcgcgct tccgagatga agcacgtcaa ggcgatctgg cagatcggca
     3481 tgcgctcgca gggcagcgcg cgcctcgaag aggcgcaggc cgccgccgcc tacggctcga
     3541 acatcgtgcc ggcccacgag gtgcacgatg ccggcatgga cgccgtgctc gagcgcattc
     3601 ccgacggcgg gcgctattac atcaccatcg acgcggacgg gctggacccc acggtcatgc
     3661 ccgcggtcga gggacccgcg ccgggcggcc tgacctttca tcaggtgcgc aaactgatcc
     3721 acggcctggt gcgcaagggg cgggtggtcg gcatggacat cgtcgagatc acgccgtcca
     3781 tcgacgtcaa catgattacc tgcatcaccg cgggccgctt gatggtcaac ctgatcggcg
     3841 ccgccgtgcg tgccgattat ttcgacgcgc ccaggctgta gcgccattcc atccataaat
     3901 cctgctgttc catttttcgt tcaatacaag gggaatgaat catgaagcgc attacgtgca
     3961 ccgcgcgggt gatgtttgct gtggggctgg cggcgatcat gctgctggcc tcggcggggg
     4021 cttcggccga gacgacgctg gagcgcatcc gcaggacggg cgagctgacc gtcggcaccg
     4081 aggcggccta cccgcccttc gaattcgtca aggacggcaa gatcgtcggc tatggctccg
     4141 acattctcgc ggaagtcgcc aaggagctgg gcgtcaagca attcaaccag ctggacctgc
     4201 catggcaagg catcctgccc ggcgtgctgg cggggaaatt cgatttcgtc gccaccacgg
     4261 tcggcatcaa cgaggagcgc gccaagcgct acgcctacac gatgccgatt gccgacggcg
     4321 cgccttattt cctgcagcgc aaggacgacg gcgccaagtt ggagacgggc ctgaacggca
     4381 aggtcgtggc gacgcagctc gcctcgtcca cggaaccgat cgcccgccag ctggacgccc
     4441 agctcaaggc cgccggcggc agcgggttca aggagctcaa gctgtatacc tcctttcccg
     4501 agacctacgt cgccttggcc aacggggagg tcgactgggt gttgcagggc ctgcccagcc
     4561 tgtccgtcgt cgtcaaggag catccggata cgttcaagct gggcggcccg gtcccggtca
     4621 cgggcttgta tacctatctt gcctgggtgg ctcggcccga agacaaggac ttgcgggatt
     4681 tcatctccgg cgtgatccgc aagatgcgcg acgatggccg cctgggagcg ctgcagaaga
     4741 aatggttcgg cttcgagatg aagattcccg attcgggcta tctgccgccc ggggcggtgt
     4801 gagcgcccgc cgcggagcgc cgggatgaaa ttcaacttcc attacttgct ctcggtgctg
     4861 ccgccactgg ggtcggcggc gctggtcaac ctgcgcctgg ccgccatcgc catcgtgctg
     4921 gccttggtgc tgggcacggc gctgacggta ctgcgttccc gaaagatcgg gccggtcaat
     4981 gcggcgatcg cggtcttgat cagcttcgtg cgcgggacgc cgctgctgat acagatcttc
     5041 atttttttct acgtgctgcc cgcggtggga ttgaatcttt cggcgcccgt ggcgggcgtg
     5101 gccgcgctgg cgtgcaacag catggtgttc gtcagcgaaa cgctgcgggc cggtctcggc
     5161 acgctggatc cgggccagat cgaggccgcc accgcgctcg gcctgccgcc gcgcgtcatc
     5221 tggataaagg tgatcctgcc gcaggtattg cggcgctcgc tgccgatgct ggccaacgag
     5281 gcgtccatcg tgatcaaggc gacggcgctg ttgtcggtga tcaccgtggt cgatgtgctg
     5341 cgcacggccc agcagatcgc cggcgcatcg ttccggccat tcgagacctt gttcggcgcg
     5401 gccctgatgt tcgcgctcat caacatgctg ctcagcctct gcaccaggat ggtggagaag
     5461 cgggtcatgc tgcgggtgtg aggtagcgat gcgattcgaa ccgtccatca tcctcgacaa
     5521 tgcgccgctg ttcctgaagg gcgcgttgct gacgctcgaa atatcggcct gcgcgctgag
     5581 catcggcttt gtgctcggcg tcgcaatcgc gctattgcgg cttgccccgt cgcgggtgct
     5641 gcacggtttc gcggggggct acatcgccgt gctgcgcagc gtgcccttca tcattttcct
     5701 gttcctggtt tattacgggc tgccgttttc gggcatacgg atacccgcct tgaccacggg
     5761 catcgtttcc gtcgccctgt tctccagtcc ttattacgcg gaagtcatcc gttcggccat
     5821 tcaggcgttg ccggccgggc agtacgagtc ggcgcgggtg ataggcatgt cccccgcgca
     5881 agcgatgcgg cacgtgatcg cgccgcagat cgttcccgcg ctgattgcgc cggccacgaa
     5941 cgtgaccttg accatgatca aggactcggc cgtcctgtcg tcgatcacga ttcccgaatt
     6001 gagctaccag ggactgatcg tgcaaggaaa caccttcgcg cctttcgagg tgttcgcggc
     6061 ggtggccgga ctgtattggc tgatttcgat ggcgatcgcc gaattttcgc ggcggctcga
     6121 actgcgcgtc gcgggcatgc gggaacggcg cgcgacgcgc aatgcgctgg cggaccgata
     6181 cctttccctg gacaagcgga cggcagcatg agcccctcga agcccgcctt gcgattgtcc
     6241 ggcatcgtca agaatttcgg gccgacgacc gcgctcaatg gcctgtacct cgacgtgcac
     6301 gagggggaga tcgtggccct gctgggtccc agcggttgcg gcaagagcac cttgctgcgc
     6361 tgcgcgacct ggctcgagca acccgacgac ggcttcatcg aggtggcggg caagcccttc
     6421 ggccgcatgc gcaccacggg cggcgtggtc aggcatcaga ctcgtgaaga aatcgatcgc
     6481 atacggccca ggataggcct ggtcttccag cagttcaacc tgtggccgca cctgaccgcg
     6541 ctggacaaca tcgtttgtgc ccaatgcgtg gtgctgggcc gcgagcggga cgcggcgcgg
     6601 cggcgcgggc ttgaattgct ggaccggctg ggccttgcca cgctggcgca tcgccgcccc
     6661 gcggaactgt ccggcggcca gcgccagcgc gtggcgatcg cgcgggcgct ggccatggac
     6721 ccggccgtca tgctgttcga cgagcccacc tcggcgttgg atccggaact ggtcggcgag
     6781 gtgttgtcct tgctgaaact gctggccggg cagggcacga cgatgctggt cgtcacgcat
     6841 agcgtgagtt tcgcggcggc attggccgac cgcatcgtat tcatggacgc gggatgcatc
     6901 gtcgaggaag ggccgccgac aagtttgctc aaggcgcctc gatcggaaag attgcgcgtc
     6961 tttctcgaac acaccttgtc cgagcggcct gcctggccgc cggttgttgc acaggaggct
     7021 tgaaccatgc agcgtgtcaa tcgtcccttc gtgggcattc cgtctttcct gcgatcccgg
     7081 gtctgcgaag acctgacccg gctcgacgcg gacattgccg tcttcggcgt gcctttcgac
     7141 gaaggctcgc cttatctgcc gggaacacgc atggggccgc gcgccctgcg cgagcattcg
     7201 atgcgctttt gcggcggatc ggggggctac tacaacccgc agacgcgcgg ccagtacctg
     7261 gtcgaggaaa tgaacctggg catgatcgcg gacgtgggcg acgtggatgt ccatccgacc
     7321 aatcccgagc gcactttcca gaacgtcacc gagaccgtgc gggccattct ggaccgggga
     7381 gcgctgccta tcggcttggg cggggaccac tcgatcacct atcccatatt ccgcgctttc
     7441 gacgagccgc tgcacgtctt gcatttcgac gcgcacaccg acttcgcccc gttcgagaaa
     7501 gacctgagat tgaccaacgg gcacgccttt cgccacattg ccgccatgcc caataccctg
     7561 agcctgacgc aggtcggcat ccgcagcctg cggcattcgc gcgcccagat cgacgatgta
     7621 aaaacctcgg ggaaccgggt cgttccgatc aatgactttc gcgagatggg gccggaaggc
     7681 atcgcggcat tattgccgga gggttcgcgg gtctacgtca cggtggacgt cgatgtgctg
     7741 gacatgtcgc tgatccccgg ctgcgtttcg gccgagcccg atggcatgag ttacgcggac
     7801 ttgcgcgaca gcctgaaggc catcgccgcg cgccatgacg tggcgggctt cgatttcgtc
     7861 gaagtcaatc cgctgctcga cgtcgcgacg ggcattacct cgtatctggg cgcgctggtg
     7921 gtggtggaat ttctcggcaa tatctgcgcc cagccgcgct gggcggcgcg gcgcctggcc
     7981 cgggcgcgat aaaaggagcg catggcaatg aagagggatg acgccgaagc gctgcatgcg
     8041 tacgacaacg ccgcggaact gggacgcagg tctgcgatac gtggatattc aacgcggatc
     8101 acctggatga gttcctgtcg ttcgagcatg cccgggcgct caatccggtc gaagtggcct
     8161 ttaccgtgct ggcgtagcgc tcggtcgcag caggtatgat cttggcatga gttccatgcc
     8221 gcaactcgcc cagctgccgt ccgatgccta caccaagcgc cgccaggtgc ttggtgagtt
     8281 cgtgcgcaac gcgcgcgccc gcatcacgcc gcagatggcc gggctgcccg aaggcgcgcg
     8341 gcgccgcacc cccggcctgc ggcgcgagga agtggcgcag ctttccggca tcagcgtcac
     8401 ctggtacacc tggatagagc aggggcgcga cgtgtcggtg tcgcccaccg tgtgggcgcg
     8461 catcgccagc gtgctgcacc tgggcaaggc cgagcgcgcc tatcttttcg agctggccga
     8521 atgcgccgac ccgcagcagc cgcgcgaggc gcccgaaggc gtggcggagc gcctgaaagc
     8581 ctgcgtcgat gcgatcagcg cgccggccta tgtgctggac catgcctgga acgtgctggc
     8641 gttcaacgaa gccatgcgcg acctgttcga cgactggccg gcgcgcgagc cccggcccaa
     8701 tctgctgcgc tacatttttc tcgaccccgc cgcccgccgg ctggtggtgg attgggagca
     8761 gcgcgcccgc cgcgtggtgg ccgagttccg cgccgatgcg gcgcccaacc tggacgtgcc
     8821 cgacgtggcg gccctggtgg atgcgctcga tcgcgagagc ccgatgttcg cccattggtg
     8881 gacgcgccat gccgtgatcg agcgtgaagg cggttttcgc gatttcgacc acccgcgccg
     8941 cggccggctg gccttccagc agatcacctt ccgcctggcc acgcatccgg agctgaagct
     9001 ggtgatgctg ctgcagccgg agtatgcgcg agcgcttgct atcccggact aggcgtgggc
     9061 aggttcgaca tccagccgtt tgccggagtg ttcgagctgc cacaggtcat gttcgatctg
     9121 catctgcagc cacatgatgg cggtaggcgc cggtatccac ttcgacaggc gtacggccaa
     9181 ttcgggtgtc acgctcgagc ggccattgat gaaccgcgag aactgcacgc gactgacgcc
     9241 caattgccga gcggcttcag tcacgctcag tcctagctca gggaggatgt catccttcag
     9301 gatggagccg ggatgaggcg gattgtgcat gcgcatgatg gttttttgtc tagtgctaat
     9361 cctgatagtt caccaggatg gcgccctctg gagcctgtct cgaaaaacag ttgcagtcct
     9421 ttgtggcgaa aactctggat catgcgtgtc gagagaggcg tatcttgtat ctatacaaga
     9481 tacaacatgg gaagcaaaga aagattgaat gatcatccag tgaagccggt actagtaggt
     9541 cgaaagtgcc ggttttgatc cgtctcggga tatacggatt gacaggcggg ccatggcggc
     9601 cggcaaagtg ctatggcgag ttttgataga ccacttgtcc atccacggca tagagccggc
     9661 aatccacgcc gcgatgttcg cggcagctgt ccagggcctt ctgcgcggcg ttcttgccgg
     9721 attggcggct ccaggcgccg ttttccgaca gggcgaaggc gcggccgttt ttggggtcca
     9781 tcagcaggaa gcgttcgtat ccgctgcgta tggtgttgcg gctgcgctgg aagggtacgg
     9841 cgtcggcatc gccgagcgcg gcggcgccgt cggaatgcgg cggttgcacg atgtcccagg
     9901 tcagccccag gcgctggaag aaccggccga cgatcggcac ccagatgtcc agcccgtcga
     9961 agttctcgaa gagatggtgc gaggtgtcct tggcgaagac gcccgtgtcg acataggtcg
    10021 agcgcgggtt gcccgcgtgg taggcgttga agaagcggcg cgacagtttg ccgtcgcccc
    10081 agtaggcgtc gttgtcgccg tagatgaaca gcgccggcac gtttgtgttg gcgccgtagg
    10141 tacggaaggc ggcggtattg cggttggccc agtcgcggca gttttcctgg cgcaggccgc
    10201 ccgcgaagtt gacgatgccc agcaggcccg gcacgtcgcg cacgcccagg gccgtcgaga
    10261 ccagccctcc ctgcgatgcg ccgacgacga cgatgcgccg cgtgtcgata tagggcaggg
    10321 tgcgggcgta gtcgatggcg gccttgacgt cgtcggccca gaagtcgctg agcgtggcca
    10381 gcggctgcct gcagccgctg aggtagctgc cgccggacga gccgaatccg gcccgcaccg
    10441 gcaccagcac ggcgtagccg cgccggacga attgcaggga ctcggggtag aagcgcgaat
    10501 cgggctgttc ggacgccttg cccgggctct tgccgtggtt gatgacgacc agcggaaacg
    10561 gcccctcgcc atttggcgta tagagcgtcg cggtgagctg tatgtccctg ccgttttgcc
    10621 tggcaggcag cctgaccgtc tcgtcgcgca tgtcggccgc ttgtgccgcc gcggcggcaa
    10681 gcagcgtcag gatggcgcaa aaaaggcccc cacgccgcac ggctatgccg cttgctgccc
    10741 cccagggggg gcgtttttgc cttggggcgg cccggcggca aaagaagagt cgagtcatgg
    10801 ctaaagctta gccggttttg ctagggggac gcatccggca gcccggcggc ggccaggccc
    10861 tccagcagct ttttcaccgg tgcgggcagg gcggcggagg gcagcgcgcc cacggccagc
    10921 cattgctcgg gcaggccggc ttcgcgcagg gcggcctgcc cggataccgt gacgtaccag
    10981 ggctgcacat gcagcttgta gtgcgtgaaa acgtgagtga agcccgccag ccggtagtgc
    11041 tcggcgggtt ccagccccag ggcgcgggcg gccgcccgcg ggtcgccgcc ggcctcgaac
    11101 tccggcaggc tccacaggcc gccccagatg ccgggcgagg ggcgttgctg cagcagcacc
    11161 gcgccgccgc gcgccaggat cagcatggcc gtctcgcgct cgggcacggt cttgcgcgcc
    11221 ttgggggtgg gcagttccgc ctgccggccg tcgcgccggg ccacgcaggt gccggccacg
    11281 gggcagcgcc cgcaggccgg tttgccgcgc gtgcagagcg tggcgcccag gtccatcagc
    11341 ccctgggtgt aggccggcat gtcgagcgcg ggcgcggccg caacctgcgc gtcggccaat
    11401 tgccacagct gctggtcgac ggcccgcttg gcggggtcgc cctcgatgcc gaaatgccgc
    11461 gtgaacacgc gcttgacgtt gccgtcgagg a
//
LOCUS       DASWPO010000335         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2402285, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000335 DASWPO010000000
VERSION     DASWPO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402285"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3196
                     /locus_tag="VGC69_17800"
     CDS             <1..>3196
                     /locus_tag="VGC69_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0297188.1"
                     /translation="CGIVMSIEDGKVIKVAGDKQHPANFGRLCTKGQTCAQAIGAPGR
                     LEHAYLRAEREQEPARVPLERAIAETAQRLRAVIDAHGPDAVALYVSGQMSMEAQYLA
                     NKLAKGFIGTNNIESNSRLCMASAGSGYKLSLGADGPPGSYQDFDHADLFFVIGANMA
                     DCHPILFLRMMDRVKAGAKLVVVDPRRTATADKASLYLPIKPGTDMALLNGLLHLLVK
                     NGHTDPAFIAEFTEGWEAMPGFLEDYAPDKVAEITGLPEADIRRAADWIGTAREWMSC
                     WTMGLNQSTHGTWHTNAICNLHLATGAICRTGSGPFSLTGQPNAMGGREMGYMGPGLP
                     GQRTVLSDADRALVEDMWDIAPGTLRQDTGGGTVALFDDMAAGKVKACWIICTNPVAT
                     VPHRQQVIAGLRAAELVITQDAFLDTETNRYADILLPGALWAEAEGVMVNSERNLTLM
                     QQAVAPPGEALADWQIIARVACAMGYGHAFDYPDAAAVFAEIQRFHNPKTGYDIRGAS
                     YARLRQTPLQWPCAPGAAHDRNPIRYRNDGTRPRIAFATAHGKAVFLPRPYAPPAEMP
                     DADFPLVLNTGRLQHQWHTLTKTGKIATLNKLNPGPFVELHPEDAAVLGIVDKDRVEV
                     RSRRGRAVLPAVVTGRVRPGNCFAPFHWNDVFGDELTINAVTSDAIDPISQQPELKFC
                     AVALARVEEAPAAAATPLRGDQQSAAASAPPAPKEHHTMQQINALARILGIDIATPLA
                     LDAREQLYLQGFLLGLQTEEARQLGGVPTLPATAPFEPSKRLVLDGLLAGIFSRTWPP
                     GEPALGAPSSTGAAQSILVVWASQTGNAEGFADICATALKGEGRSVRAAAMDTLSVAD
                     LARADVLLCLASTFGDGDPPDNGAALWQALQAPDAPALGAQRFGVLAFGDSSYDQFCG
                     FGRRLDARLEALGARRIAPRVDCEPEYEGAAQGWLASVCQALLALTTVAQSAPAAQSV
                     LVVAGDGEATALDAAPEAKHPAAWSKQNPLRTRLLVNRVLNAQGADKETRQFVFDLQD
                     SGLHYEAGDALGVWPSNCPELVADMLTALKLPASA"
BASE COUNT          531 a         1173 c         1043 g          449 t
ORIGIN      
        1 ctgcggcatt gtcatgtcca tcgaggatgg caaggtcatc aaggttgcgg gcgacaagca
       61 gcacccggcc aacttcggcc gcctgtgcac caagggccag acctgcgcgc aagccatcgg
      121 cgcgccgggt cggctggaac atgcctacct gcgcgccgaa cgggaacagg agccggcccg
      181 cgttccgctg gagcgcgcca tcgccgaaac cgcgcagcgc ctgcgcgcgg tgatcgacgc
      241 ccacggcccg gacgcagtcg ccctctacgt ctcaggccag atgtcgatgg aggcgcagta
      301 ccttgccaac aagctggcca aggggttcat cggcaccaac aacatcgagt cgaactcgcg
      361 cctgtgcatg gccagcgcgg gcagcggcta caagctctcg ttgggcgcgg atggcccgcc
      421 gggcagctac caggacttcg accacgccga cctgttcttc gtcatcggcg ccaacatggc
      481 cgactgccac cccatcctgt tcctgcgcat gatggaccgg gtgaaggccg gcgcgaagct
      541 ggtcgtggtc gacccgcgcc gcaccgccac ggccgacaag gccagcctgt acctgcccat
      601 caagcctggc accgacatgg cgctgctcaa cggcctgctg cacctgctgg tgaagaacgg
      661 ccataccgac ccggccttca tcgccgaatt caccgaaggc tgggaggcga tgcccggctt
      721 cctcgaagac tatgcgcccg acaaggtggc cgaaatcacc ggcctgcccg aggccgacat
      781 ccgccgcgct gccgactgga tcggcaccgc ccgcgaatgg atgagctgct ggaccatggg
      841 cctgaaccag agtacccacg gcacctggca caccaacgcc atctgcaacc tgcacctggc
      901 cacgggggcg atctgccgca ccggcagcgg gccgttctcg ctcaccggcc agcccaatgc
      961 catggggggc cgcgagatgg gttacatggg gccgggcctg ccggggcagc gcacggtgct
     1021 gtccgacgcc gaccgcgcct tggtcgaaga catgtgggat atcgcacccg gcacgctgcg
     1081 ccaagacacc ggcggcggca cggtggcgct gttcgacgac atggccgcgg gcaaggtcaa
     1141 ggcctgctgg atcatctgca ccaacccggt ggcaacggtg ccccaccggc agcaggtgat
     1201 cgccggcctg cgggccgccg agctggtaat tacgcaggat gccttcctcg acaccgagac
     1261 caaccgctac gccgacatcc tgctgcccgg cgcgttgtgg gccgaggccg agggcgtgat
     1321 ggtcaactcc gagcgcaacc tcacgctgat gcagcaagcc gtcgcgccgc ccggcgaagc
     1381 gctggccgac tggcagatca tcgcccgcgt ggcctgcgcc atgggttacg gccatgcctt
     1441 tgactacccc gacgcggcgg cggtgttcgc cgaaatccag cgcttccaca accccaagac
     1501 tggctatgac atccgtggcg caagctatgc gcggctgcgg cagacgccgc tgcaatggcc
     1561 ctgcgcgccc ggcgcggcac acgaccgcaa ccccatccgc taccgcaacg acggcacgcg
     1621 cccgcgcatc gccttcgcca ccgcgcacgg caaggccgtg ttcctgcccc gtccctacgc
     1681 gcccccggcg gagatgccgg acgccgactt tcccctggtg ctcaacaccg gccgcctgca
     1741 acaccagtgg cacacgctga ccaagaccgg caagatcgcc acgctcaaca agctcaaccc
     1801 cgggcccttc gtcgagctgc atcccgaaga cgcggccgtg ctgggcatcg tggacaagga
     1861 ccgcgtggaa gtgcgctcgc gccgtggccg cgccgtgctg ccggccgtgg tgaccggccg
     1921 cgtgcgcccg ggcaactgtt tcgccccctt ccactggaac gacgtgttcg gcgacgaact
     1981 gaccatcaac gccgtgacca gcgacgccat cgacccgatc tcgcagcagc ccgaactcaa
     2041 gttttgcgcc gtcgccctgg cgcgtgtcga ggaagcaccg gcagcagcag caaccccctt
     2101 gcgcggcgat caacagagcg ccgcagcctc cgcgcctccg gcccccaagg agcaccacac
     2161 gatgcagcag atcaatgcgc tggcccggat cctgggcatc gacatcgcta ccccgctggc
     2221 gctggacgcc cgcgaacagc tctacctgca aggcttcctg ctcgggctgc agacggaaga
     2281 agcccgccaa ctgggcggcg ttcccacgct gcccgccacc gcgccgttcg agcccagcaa
     2341 gcggctcgtg ctcgacggct tgctggccgg gattttctcg cgcacgtggc caccgggcga
     2401 acccgccctg ggcgcgccct catccacagg ggctgctcag tcgatcctcg tggtgtgggc
     2461 ctcgcaaacc ggcaatgccg aaggctttgc cgacatctgc gccacggcgc tgaagggcga
     2521 aggccgcagc gtgcgggctg cggccatgga cacgctatcg gtggccgatc tggcgcgggc
     2581 tgatgtgctg ctgtgcctgg ccagcacctt cggcgacggc gacccgcccg acaacggcgc
     2641 cgcgctgtgg caggcgctgc aggcgcccga cgcaccagcc cttggcgcac agcgctttgg
     2701 cgtgctggcg ttcggcgact ccagctacga ccagttttgc ggcttcggcc gcaggctcga
     2761 cgcgcgcctg gaggcgctgg gcgcgcgccg catcgcgccc cgcgtcgatt gcgagccgga
     2821 gtacgagggc gcggcacagg gctggcttgc atcggtctgc caggcgttgc tggcgctaac
     2881 cacggtggcc cagtccgcgc ccgcagcgca gtccgtgctg gtggttgctg gcgacggcga
     2941 ggccactgcc ctcgatgcag cgccggaggc gaagcatcca gcagcctgga gcaaacagaa
     3001 ccccttgcga acccgactgc tggtgaaccg ggtgctcaat gcccaggggg ctgacaagga
     3061 aacccgccag ttcgtcttcg acctccagga ctcggggctg cattacgagg ctggcgacgc
     3121 cctgggcgtg tggcccagca actgcccgga gctggtggcc gacatgctca cggctctgaa
     3181 gctgccagcg tcggcg
//
LOCUS       DASWPO010000336         8152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_617334, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000336 DASWPO010000000
VERSION     DASWPO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617334"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGC69_17805"
     CDS             <1..210
                     /locus_tag="VGC69_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0297189.1"
                     /translation="REEGRVIVLVRISTQGLVEQASIDTSSGYSRLDDSALAAARQAR
                     FKPLMRNGVALPALAKLPFDFKMRN"
     gene            213..1058
                     /locus_tag="VGC69_17810"
     CDS             213..1058
                     /locus_tag="VGC69_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0297190.1"
                     /translation="MSISTLLTNTPLAAVAQQVLAQVSTASTGAAAAPAEPATGVLHF
                     VSQSDAVGQALFFILLAMSLVTWYLIVVKTVSNLRMRSRARDFLNSFWNASSLEQVQN
                     EIVTHGARDPFSHLTSHALHAQSHHAKFGATKLEESGSNGDFVTRTMRKVIDEETAKL
                     ENGLTILASVGSTAPFVGLFGTVWGVYHALVGIGMADGMTINRIAGPVGESLVMTGLG
                     LAVAIPAVLAYNAFVRNNRVFLSRLDAFAHDLFAFLTTGRQVEVSDGKVRALRRQGGE
                     QHGGK"
     gene            1062..1559
                     /locus_tag="VGC69_17815"
     CDS             1062..1559
                     /locus_tag="VGC69_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0297191.1"
                     /translation="MAFGSFDSKGAGSHTVSEINMVPLIDVMLVLLVIFIITAPLLAH
                     SIRINMPQVSAEQIQEDPKTIDLAIDADGKLFWDEKPVTMDDLPGRFQSSAQDQPQPE
                     IRIRADENTRYDVLAKVMGAARHAGMGKIGFITTPAPQQASQPAPQPAGPDKPQHRGG
                     PAISQ"
     gene            1603..2274
                     /locus_tag="VGC69_17820"
     CDS             1603..2274
                     /locus_tag="VGC69_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0297192.1"
                     /translation="MRVLVIEDDTTLGHALQEFLSDQGYAVDWLTEGDKVQGAMVGQS
                     YELILLDLNLPGLGGLDVLRQLRQDGNQVPVLILTARDGLDDRVAGLDAGADDYVTKP
                     FDLPELAARVRALARRRAGQAQPMIEVGPLVFDTIGREVRVNGQRLALSVRELSVLEM
                     LMARVGRVVTKRQIVNSLSAWDADFSENAVEVYVYRLRKRLESTGASIQTVRGFGYLL
                     DVESV"
     gene            2339..3460
                     /locus_tag="VGC69_17825"
     CDS             2339..3460
                     /locus_tag="VGC69_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0297193.1"
                     /translation="MPDILPKPQSASWRAAWRLPEGSLARLLVARLMPPLTVLVLLDL
                     AATWVITNKIDMSVWMLEDFFWLMVAGQVLLIVSFAGVIVHGVRSGLRSVNHLADQIR
                     QRSIEDMQPMAVANLPAEIAPLVTHINDLLERLDSSLSAQRRFIGHAAHQLRTPLSGL
                     KLESELMLARPLPDDVRVRAERIKVVSDRMIRLGQQLLVLARSNPDAQPQDHFVRLDL
                     CEWVRASGAEWVPRALAAQVDVDLVAPDAPVAIDGDPLLLDELLGNLIDNALRYGKQH
                     GRITLTVGANPPSLTVEDDGPGIPPDESERVFEAFYRSPSAAAGGSGLGLAIVREIAN
                     AHGAWWKLTSRPEYAGTRLTVVFPGPRKGTQLSRHERHV"
     gene            3457..5340
                     /locus_tag="VGC69_17830"
     CDS             3457..5340
                     /locus_tag="VGC69_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY0297194.1"
                     /translation="MSASPTASHSSSRKALVLGALGVVYGDIGTSPLYTLKVCLTEFT
                     SVRPEYVLGVLSILFWLLMVVVSFKYMVLVLRADNRGEGGTLALLELAARGVTGHKRA
                     ALVALGIFGAALFYGDSMITPAISVLSAIEGIGVVSNRFDHWIIPVSLVVLIALFSLQ
                     SHGTATVGKLFGPVMGLWFLTLGALGLWQIWQHPQVLVALNPMVGLQFVMHHSLASFV
                     LLGAVVLALTGAEALYADMGHFGRPAIRTAWFWFVLPALVLNYFGQGALLLHNAKALQ
                     NPFFLMAPDWGVVPLVVLSGAATVIASQAVISGAFSMTRQAVQLSFWPRMQILHTSVL
                     EKGQVYLPQVNALLLVAVLVLVLVFKTSDNLAAAYGFAVTGTMLSTGVLVFMVLPRGA
                     TGGKRLAWILVLSVLLVIDLLLFSANVLKIHEGGWLPLGVGIIVFVLMTTWRQGRRLL
                     SDLQARDRQPLKEFMLQLEEYPPTRAHGTAVFMTMSSGFVPPALLHNLKHNKVLHEQV
                     IFLSIKVSDVPFVAEDERFQVERVSHSSWQITVHYGFKEDSDVPDSLRQVGEAYPELD
                     LEPMRTSFFLSRQTVVGGKRAVMPRWRRAIFAFMARNSTRSTKFFKIPPNRVVEMGMQ
                     IEL"
     gene            complement(5404..6609)
                     /locus_tag="VGC69_17835"
     CDS             complement(5404..6609)
                     /locus_tag="VGC69_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0297195.1"
                     /translation="MQKIVIVGGGHAASQLCASLAEAGRQGDVTLVSAEPDLPYHRPP
                     LSKAYIKDAGAALQLLRPESAYAGMALHLGARVERIDPAARSVTLGTGQSLPYDTLVL
                     ATGTRARRLPGLPDDIGNLHYLRTIGDARRLRAALADAPSVTVLGGGFIGLEVAATAA
                     LLGKPVTVFEAADRLLARAASPEVSQHIAHTLRQAGVALQLACGPIQFERQGPRIRAL
                     TWQGGTHPVDLLVAGIGAEPETGLAQSIGLACDNGIAVDGHLRTADPHIHAIGDCTSF
                     PYARWNQRLRLESVQNANDQARTLARVLLGQDTPPYNALPWFWSDQGALRLQIAGLAP
                     AGAQRLRRPGARPESFSILHFAQGRLACVESINAPADHIAARKLLEKDALPAAEVLAD
                     PAIPLKRHA"
     gene            6881..8029
                     /locus_tag="VGC69_17840"
     CDS             6881..8029
                     /locus_tag="VGC69_17840"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0297196.1"
                     /translation="MSKISRRHLLGLLGASVALPGLVKAQDSSPIRIGCMLPLSGNGS
                     IEGNQVYKGVQLAVADANAAGGVLGRKIELATEDDESNTAKGATAMRKLVERDHAPFV
                     VGTYASAVVVAAMKVAKELKVPLLSGGSTGAIATDANTPGDPWFFRAWADSNGQGFDT
                     ATAIVKRFGAKRVAILHDTTGYGVDLAKQTTDVVVKAGGEIVANESYNIGDQDFSTLL
                     TRIRTLKPDVVYIGGWAADGATIVRQAASVGVRAQFVGSGSMLSDDFIKLAGPASEGF
                     AVATAYEISTPNEVGRAFGERFMKAYHYEPGMLNVLGYYSTAIGLEAMRRVGKPDGAA
                     IQQMLIHGMKDFKIGLGPEGTTAAFNEKGSVDYPYFMAIIKNGKRTLN"
BASE COUNT         1301 a         2695 c         2742 g         1414 t
ORIGIN      
        1 cgcgaagagg gccgggtgat cgtgctggtg cgcatcagca cgcaaggcct ggtcgagcag
       61 gccagcatcg atacgtcttc cggttattca cgcctggacg attccgccct ggcggcggct
      121 cgccaggccc gcttcaagcc gctgatgcgc aatggcgtgg ccttgcccgc cctggccaag
      181 ctgcctttcg atttcaagat gaggaactag taatgtccat ctccaccctc ctgaccaata
      241 cgccgctggc agccgtggcg caacaggtgc ttgcgcaagt ctccaccgcg tcgacggggg
      301 ccgccgcggc gccggccgag cctgcgacgg gcgtgctgca tttcgtgagc cagagcgatg
      361 ccgtcggcca ggcgctgttc ttcatcctgc tggccatgtc gctggtgacc tggtacttga
      421 tcgtggtcaa gaccgtgagc aatctgcgca tgcgcagccg cgccagggac ttcctcaaca
      481 gcttctggaa cgccagctcg ctcgagcagg tgcagaacga aatcgtcacg cacggcgcgc
      541 gcgatccctt ctcgcatctc accagccatg ccctgcatgc gcagagccac cacgccaagt
      601 tcggcgccac caagctggaa gagtcgggca gcaacggcga cttcgtcacg cgcaccatgc
      661 gcaaggtcat cgacgaagaa accgccaagc tcgagaacgg cctcaccatc ctggcctcgg
      721 tgggctccac tgcgccgttc gtgggcctgt tcggcacggt gtggggcgtg taccacgcgc
      781 tggtgggcat cggcatggcc gacggcatga cgatcaaccg catcgccggt ccggtgggcg
      841 aatcgctcgt catgaccggc ctgggcctgg cggtggccat tcccgccgtg ctggcctaca
      901 acgccttcgt gcgcaacaac cgcgtgttcc tgtcgcggct ggacgccttc gcccatgacc
      961 tgttcgcctt cctgaccacg ggtcgccagg tggaagtgtc cgacggcaag gtgcgggcgc
     1021 tgcgccgcca gggcggcgaa cagcacggag gcaagtaagc catggccttc ggcagcttcg
     1081 acagcaaggg ggcgggcagc cataccgtct ccgagatcaa catggtgccg ctcatcgacg
     1141 tgatgctggt gctgctggtg atcttcatca tcacggcgcc gctgctggcg cattcgatcc
     1201 gcatcaacat gccgcaggtc agcgccgagc agatccagga agatcccaag accatcgacc
     1261 tggccatcga tgctgacggc aagctgttct gggatgaaaa gccggtgacg atggacgatc
     1321 tgcccggccg cttccagtct tcggcgcagg atcagcccca gccggagatc cgcatccgcg
     1381 ccgacgaaaa cacgcgctac gacgtgctgg ccaaggtcat gggggccgcg cgccacgccg
     1441 gcatgggcaa gatcgggttc atcaccacgc ccgccccgca gcaggcgtcg caaccggcgc
     1501 cgcaacccgc cgggcccgat aagccgcagc accgcggcgg tcctgcaata agtcagtaac
     1561 aagtagtaca cgtcggccgt cggatcgcgc tagaatcgaa agatgcgagt tctagttatc
     1621 gaagacgaca ccaccctggg ccacgcgctg caggagttcc tgtccgacca aggctatgcc
     1681 gttgattggc tcaccgaggg cgacaaagtc cagggagcca tggtcggcca gtcctacgag
     1741 cttattctgc tcgatctcaa cctgccgggc ctgggcggcc tggacgtgct gcgccagctg
     1801 cgccaggacg gcaaccaggt gccggtgctt atcctcaccg cgcgcgacgg cctggacgac
     1861 cgcgtggcgg gcctggacgc cggcgccgac gactacgtca ccaaaccctt cgacctgccc
     1921 gagcttgccg cccgggtgcg cgcgctggcg cgccgccgcg ccggccaggc acagcccatg
     1981 atcgaggtcg gtccgctggt gttcgacacc atcggccgcg aagtgcgcgt caacggccag
     2041 cgcctggccc tgtcggtgcg cgagctgtcc gtgctggaaa tgctcatggc gcgcgtcggc
     2101 cgggtggtca ccaagcgcca gatcgtcaac tcgctctcgg cctgggatgc ggacttcagc
     2161 gagaacgccg tcgaagtcta tgtttaccgc ctgcgcaagc gcctggaaag caccggcgcc
     2221 agtatccaga cagtgcgtgg cttcggctac ctgctggacg tggaatcggt gtaaacgcca
     2281 tgtctgcctg acccggcaga catgagccga ctgcgcctcg cacgcagccc ccctatatat
     2341 gccagacatt ctccccaagc cgcagtcggc ctcctggcga gccgcttggc ggctgccgga
     2401 ggggtcgctc gccaggctcc tggtcgcgcg gctgatgccg ccgctcacgg tgctggtgct
     2461 gctggacctg gcggccacct gggtcattac caacaagatc gacatgtcgg tctggatgct
     2521 ggaagatttc ttctggctga tggtggcggg gcaggtgctg ctcatcgtgt cgtttgccgg
     2581 cgtcatcgtg catggcgtgc gttcgggcct gcgctcggtc aatcacctgg ccgaccagat
     2641 ccgtcagcgg tccatcgaag acatgcagcc catggcggtg gccaacctgc cggccgagat
     2701 cgcgccgctc gtcacccaca tcaatgacct gctcgagcga ctggattcat cgctgtcggc
     2761 ccagcgccgc ttcatcggcc atgccgcgca ccagttgcgc acgcccctgt cgggcctgaa
     2821 gctcgagtcc gaactgatgc tggccaggcc cttgcccgac gatgtccgcg tgcgggccga
     2881 gcgcatcaag gtcgtcagcg accgcatgat ccgcctgggc cagcagttgc tggtgctggc
     2941 ccgctccaat cccgacgccc agccgcagga ccacttcgtg cgcctggacc tgtgcgaatg
     3001 ggtgcgcgcc agcggcgccg agtgggttcc gcgcgccctc gccgcgcagg tcgacgtgga
     3061 cctggtggcg ccggacgcgc cggtggccat cgacggcgat ccgctgctgc tcgacgaact
     3121 gctgggcaac ctgatcgaca atgcgctgcg ctacggcaag cagcatgggc gcataacgtt
     3181 gaccgtgggc gccaatccgc cgtccctgac ggtggaggac gacggccccg gcatcccgcc
     3241 cgatgagagc gagcgggtgt tcgaggcgtt ttaccggtcg cccagcgcgg cggccggcgg
     3301 atcgggcctg ggcctggcca ttgtgcggga aattgccaat gcgcatggcg catggtggaa
     3361 actgaccagc cgtcccgagt atgctggcac ccgcctgacc gtcgttttcc ccggcccgcg
     3421 caagggcact caattgtcac gtcatgagcg gcacgtatga gcgcatcccc cacggcaagt
     3481 cattcttctt cacgcaaggc gctggtgctc ggcgcgctgg gggtggtgta cggcgacatc
     3541 ggcaccagtc cgctctatac cctgaaggtc tgcctcaccg agttcacttc cgtccgcccg
     3601 gaatacgtgc tgggcgtgct gtccatcctg ttctggctgc tgatggtggt ggtgtcgttc
     3661 aaatacatgg tgctcgtcct gcgcgccgac aaccggggcg agggcggcac gctggccctg
     3721 ctggagctgg ccgcgcgggg cgtgacaggg cacaagcggg cggcgctggt ggccctgggg
     3781 atcttcgggg cggccttgtt ctacggcgac agcatgatca cgccggctat ttccgtgctg
     3841 tcggccatcg agggcatcgg cgtggtgtcg aatcgcttcg atcactggat catccccgtg
     3901 tcgctggtgg tgctgattgc cctgttctcg ctgcagtccc acggcacggc cacggtgggc
     3961 aagctgttcg gcccggtcat gggcctgtgg ttcctcacgc tgggcgcgct gggcttgtgg
     4021 cagatatggc agcatccgca ggtgctggtg gcgctcaatc ccatggtggg cctgcagttc
     4081 gtgatgcacc actccctggc cagcttcgtg ctgctgggcg ccgtggtgct ggcgctgacc
     4141 ggggccgaag ccctgtatgc cgacatgggc cacttcggcc ggcccgccat ccgcacggcc
     4201 tggttctggt tcgtgctgcc ggcgctggtg ctgaattatt tcggccaggg cgcgctgctg
     4261 ctgcacaacg ccaaggcctt gcagaatccc ttcttcctga tggcgccgga ctggggcgtg
     4321 gtgccgctgg tggtgctgtc gggcgcggcc acggtgatcg cctcgcaggc cgtgatctcg
     4381 ggcgcctttt ccatgacgcg ccaggcggtg cagctgagct tctggccgcg catgcagatc
     4441 ctgcacacct cggtcctgga aaaaggccag gtctatctgc cgcaggtcaa tgccttgctg
     4501 ctggtggcgg tgctggtgct ggtgctggtg ttcaagacgt cggacaacct ggccgccgcc
     4561 tatggtttcg ccgtgacggg caccatgctc agcaccggcg tgctggtttt catggtgctg
     4621 ccgcgcggcg ccacgggcgg caagcggctg gcctggatcc tggtgctgag cgtgctgctg
     4681 gtgatcgacc tgctcctgtt ctccgccaac gtgctcaaga tccatgaggg cggctggctg
     4741 ccgctgggcg tgggcatcat cgtgttcgtg ctcatgacga cctggcggca gggccgccgc
     4801 ctgttgtccg acctgcaggc gcgcgaccgc cagccgctca aggagttcat gctccagctc
     4861 gaggagtacc cgccgacgcg cgcgcatggc acggccgtct tcatgaccat gagctcgggc
     4921 ttcgtgccgc cggcgctgct gcacaacctc aagcacaaca aggtgctgca tgagcaggtg
     4981 atcttcctct ccatcaaggt gtcggacgtg cccttcgtgg cggaggacga gcgcttccag
     5041 gtcgagcgcg tcagccactc cagctggcag atcaccgtgc actacggttt caaggaagat
     5101 tccgacgtgc ccgattcgtt gcgccaggtg ggcgaggcct atccggagct ggacctggaa
     5161 cccatgcgca cctcgttctt cctgtcgcgc cagacggtgg tgggcggcaa gcgcgccgtc
     5221 atgccgcgct ggcgccgggc catcttcgcc ttcatggcgc gcaacagcac gcgcagcacc
     5281 aagttcttca agatcccgcc caatcgggtg gtggagatgg ggatgcagat cgagctgtag
     5341 cggcgcgcag cggcgtggcg tgcaacaggc ggcgagggcg tgtgccccgc cgcctgttgc
     5401 ctttcaggcg tggcgcttga gcgggatggc cgggtcggcc agcacctcgg cggccggcag
     5461 cgcgtctttt tccagcagct tgcgcgcggc gatgtggtcg gccggcgcat tgatcgattc
     5521 cacgcaggcc agcctgccct gcgcgaaatg caggatggag aagctctcgg gcctggcgcc
     5581 cgggcgccgc aggcgctgcg cgccggccgg agccagcccg gcgatttgca ggcgcagcgc
     5641 gccctggtcc gaccagaacc agggcagggc gttataaggc ggcgtgtcct gcccgagcag
     5701 gacgcgcgcg agggtgcggg cctggtcgtt ggcgttctgc acggactcga gccgcaggcg
     5761 ctggttccag cgcgcatacg gaaagctcgt gcagtcgccg atggcatgga tgtgcggatc
     5821 ggcggtgcgc aggtggccgt cgacggcgat gccgttgtcg caggccaggc cgatggactg
     5881 ggccaggccg gtctcgggtt cggcgccgat gccggccacc agcaggtcga cgggatgcgt
     5941 gccgccctgc caggtcaagg cgcggatgcg cgggccttgc cgttcgaact ggatcggccc
     6001 gcaggcgagc tgcagcgcga cgccggcctg ccgcagcgtg tgggcaatgt gctgcgacac
     6061 ctcgggcgag gcggcgcgcg ccagcagccg gtcggccgct tcgaaaaccg tgacgggctt
     6121 gccgagcagc gcggcggtgg cggcgacctc caggccgatg aaaccgccgc ccagcacggt
     6181 gaccgaggga gcgtcggcca gggccgcgcg caggcgccgc gcgtcgccga tggtgcgcag
     6241 gtagtgcagg ttgccgatgt cgtcgggcag gccgggcagg cgccgcgccc gggtgccggt
     6301 ggccagcacc agggtgtcgt agggcaggga ctggccggtg ccgagcgtca cgctgcgggc
     6361 cgcggggtcg atgcgctcga cgcgcgcgcc caggtgcagg gccatgccgg cataggccga
     6421 ctcggggcgc agcagctgca gcgccgcgcc ggcgtccttg atataggcct tcgacagcgg
     6481 cggacggtgg tagggcaggt cgggttccgc gctgacgagg gtgacgtcgc cttgccggcc
     6541 ggcttcggcc aggctggcgc acaactgcga ggcggcatgg ccgccgccca cgatgacgat
     6601 tttctgcatg gtgcccagag gcgggagatt ggaaagagat cgtatttata ttcaatacga
     6661 acgtagtgtt gaaagataga tacggtttta ccaatctact cgtgaaattt cgtccagatt
     6721 acctgggata acccgtaaat atcaggctta taagggtttt ttcctaataa gaaactattg
     6781 accagggttt tgtggagagt tcattattaa atgcaaccgt attgctaata aatacggtgc
     6841 gaattcagtc cactggaact cacctatcaa aggggcatcc atgtccaaga tttcccgccg
     6901 tcacctgctc gggctgctgg gcgccagcgt tgccttgccc ggcctggtca aggcgcaaga
     6961 ttcctcgccg atccgcatcg gctgcatgct gcccctgtcg ggcaatggtt ccatcgaagg
     7021 caaccaggtc tacaagggcg tgcagctcgc cgtggccgac gccaacgccg cgggcggcgt
     7081 gctgggccgc aagatcgagc tcgccacgga agacgacgaa agcaataccg ccaagggcgc
     7141 gaccgcgatg cgcaagctcg tcgagcgcga ccatgcgcct ttcgtcgtcg gcacctatgc
     7201 ctcggccgtc gtggtggccg ccatgaaggt ggccaaggaa ctcaaggtgc ccctgctctc
     7261 gggcggctcc accggcgcca tcgccaccga cgccaacacc ccgggcgacc cctggttctt
     7321 ccgcgcctgg gccgacagca acggccaggg cttcgacacg gccaccgcca tcgtcaagcg
     7381 gttcggcgcc aagcgcgtgg ccatcctgca cgacaccacc ggctacggcg tggacctggc
     7441 caagcagacc accgacgtgg tggtcaaggc cggcggcgag atcgtcgcca acgagtccta
     7501 caacatcggc gaccaggact tctcgaccct cctgacccgc atccgcaccc tcaagcccga
     7561 cgtggtgtac atcggcggct gggccgccga cggcgccacc atcgtgcgcc aggcggcatc
     7621 ggtgggggtg cgcgcccagt tcgtgggttc gggctcgatg ctcagcgacg acttcatcaa
     7681 gctggccggg ccggcctcgg agggcttcgc cgtggccacc gcctacgaga tctccacgcc
     7741 caacgaagtc ggccgcgcct tcggcgagcg cttcatgaag gcctaccact atgagccggg
     7801 catgctcaac gtcctgggct actactccac cgccatcggc ctggaggcca tgcgccgcgt
     7861 cggcaagccc gatggcgcgg ccatccagca gatgctgatc cacggcatga aggacttcaa
     7921 gatcggcctg ggccccgagg gcaccacggc ggcgttcaat gaaaagggca gcgtggacta
     7981 cccctatttc atggccatca tcaagaacgg caagcgcacc ctcaactgat ctggcgtagc
     8041 aggaaagaga tcgtggatct tctattgcaa tacctggcca acggggtggt caacggcagc
     8101 aactacgtgc tggtggccgt cggcctcacc ctggtgttcg gcatcctggg cg
//
LOCUS       DASWPO010000337        13362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2671569, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000337 DASWPO010000000
VERSION     DASWPO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2671569"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGC69_17845"
     CDS             <1..324
                     /locus_tag="VGC69_17845"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297197.1"
                     /translation="SFIVAYSIRQDTWNRLPDDVKAAMDAASEAIEPQICKQVDDSQT
                     ATQHKLEEGGTVYNTLSDPVLKDLQAKLQGVGKVWAGELDQRGKPGTPALAEFEQLVH
                     AQGAE"
     gene            321..938
                     /locus_tag="VGC69_17850"
     CDS             321..938
                     /locus_tag="VGC69_17850"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY0297198.1"
                     /translation="MMSSLESGGALSGARARPGASWIARISEAWRAIERVCTLVAALA
                     LFATMLLVVADVFMRYALNRPFSFTYDLIGLYLLAVVFFFTISNALREHEHVCVDILL
                     QRFPTAGRRLSELVTAATGLSVFCLVGWLGFERALDSYRQGDVLAGAIPWPTWLSVLP
                     VPVGCGVLALRLALQFIGNLYSLATGADLYPLPPTVAEGRSARLE"
     gene            949..2235
                     /locus_tag="VGC69_17855"
     CDS             949..2235
                     /locus_tag="VGC69_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY0297199.1"
                     /translation="MVSGFILLLLFLLLAVGVPVGISMAFSGAVGLYSLGGLPALSGI
                     LLTAPLSTVSSYELITIPMFLLMADFVLLSGVADDLFKAAAAWVGRVPGGLGMATALA
                     GAGFGSICGTSTASAATLSSTSLPAMIRQGYDPKLAAGVVAISGTLAMLLPTSVALIL
                     FGLLAQVNIGQLLISGIVPAIFVTAAIMATIAWLVWRDPSSAPAALSVRWSERFRLLW
                     KVSPMVALFALVTGAIYLGVATPTEASAVGAFGAMLLAAAKGKLTPATLYQTVLRAAH
                     GTCMIIMILVGASIFGYFFTLSHVTQDLVGWVGALPVSRWIIIALILMGYIVLGSFMD
                     QVAILVLTVPVVLPLIKELGFDPIWFGVIKIVTAEVGMITPPVGLNCFIVARYAQRPV
                     GEVFQGTMPHFIAHIAVIAVMVAFPGIILWLPSLMN"
     gene            2251..3420
                     /locus_tag="VGC69_17860"
     CDS             2251..3420
                     /locus_tag="VGC69_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0297200.1"
                     /translation="MDSASSPTRAGPLAGLRVVEFGGIGPAPFAAMLLADLGAQVLRI
                     ERLQPADIGIKKPAQYDLLARGRSVLRVDLKHPDGVACAQAMLARSDGLIEGFRPGVM
                     ERMGLSPGACLARNPGLVYGRVTGWGQDGPLAQSAGHDLNYIALTGVLHAIGRHDAPP
                     TPPLNLVGDFGGGGMLLAFGMVCALLERQRSGRGQVVDAAMIDGAATLMTSIYGMMAA
                     GLHAAPRGGNLLDSGAPHYDVYRCADGQYLAVAPIEKKFREALFGRLGWAPDDFPDLD
                     DAANWPATRERLAALFATDTQRNWCDRLAGADACVTPVLSAAQAQRHPHHQARSTFVE
                     IDGVLQPAPSPRFSRTPPAPPQSVRQMPGGAAALLRHWGVESDRIGEWIRDGVVA"
     gene            3618..4241
                     /locus_tag="VGC69_17865"
     CDS             3618..4241
                     /locus_tag="VGC69_17865"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY0297201.1"
                     /translation="MRTLAHLLVVFALFCINSVAYAESGDILLRVRAIDMLPQVRTTG
                     TLSTLNVGLDRKFLPEIDLSYMFTNHIGTELVLGTTRSSVTSLIGGLGEQSLLPPTLT
                     LQYHFNPEGRFRPYAGAGMNYTRFYAGSLSSHGYPISMTRDSFGPVLQLGMDIGITKN
                     LFFNIDVKKIWMKTNLSLNGIPRVGSMPLGTMHIDPIFVGVGIGYRF"
     gene            4360..4872
                     /locus_tag="VGC69_17870"
     CDS             4360..4872
                     /locus_tag="VGC69_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297202.1"
                     /translation="MTYPTRRSLWVLACLLAGHAALAGEDRQNRGHDPFFQISKAIAD
                     CPAPLGPLLTEQEWISEAHHRIERGNSCWREGRCRLSNGYLYDQEIAESVQRRLDVMD
                     PATHWRDRTTLWLTLQRRFIYVQGCMSPAFDKRKFLDELAQTADVERVIDQTTAGSKA
                     ATMPYRTLTP"
     gene            4869..5009
                     /locus_tag="VGC69_17875"
     CDS             4869..5009
                     /locus_tag="VGC69_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297203.1"
                     /translation="MTKKPPTYVAHCRCGAVEVGAWSEPIAVNACYCDDCQAAARTAS
                     RR"
     gene            5745..6032
                     /locus_tag="VGC69_17880"
     CDS             5745..6032
                     /locus_tag="VGC69_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEY0297204.1"
                     /translation="MSDDHFLRIVREVAQDTSRMKLSKHARERMAERGFTTPQVYSCI
                     RRGAIHEPVHQDIRGDWKCTLRHVWAGDEIRVAIALKRNSRGDWIAVLTVF"
     gene            6043..6474
                     /locus_tag="VGC69_17885"
     CDS             6043..6474
                     /locus_tag="VGC69_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0297205.1"
                     /translation="MYHYTECGLDNVWLVNGYQIEEIDGEEYFSIEDADELHRAIGRS
                     LAEKPSLTGAEVRFLRKELGMSQRVLALLLGTTEQTMSLWERGSSVPESESRLLKVLY
                     LEKVDGNVSVNALLERIIDLDSREEDRLVFQDTECGWKMAA"
     gene            complement(7264..7605)
                     /locus_tag="VGC69_17890"
     CDS             complement(7264..7605)
                     /locus_tag="VGC69_17890"
                     /inference="COORDINATES: protein motif:HMM:NF025456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0297206.1"
                     /translation="MSKDKSTSGGAQRQRILQALALRPHNSYELRKMGCYQCPTRVIE
                     LRRQGYNIATERVTLWDDQGYRHSNVALYVLTGPGGAVMARARTLKPGLFKNETLAEC
                     SPLARLLFAGL"
     gene            complement(7607..7804)
                     /locus_tag="VGC69_17895"
     CDS             complement(7607..7804)
                     /locus_tag="VGC69_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297207.1"
                     /translation="MSTTTKQKGRDSSKSATQTTKLDDNVAKRPQYGTDEALKAALTA
                     HATTPAEYERACHLAADLAWA"
     gene            complement(7976..8051)
                     /locus_tag="VGC69_17900"
     tRNA            complement(7976..8051)
                     /locus_tag="VGC69_17900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8016..8018),aa:Thr,seq:tgt)
     gene            complement(8146..8598)
                     /locus_tag="VGC69_17905"
     CDS             complement(8146..8598)
                     /locus_tag="VGC69_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY0297208.1"
                     /translation="MGETSTKPYLIRALHEWCTDNGYTPYITVHVDAHTLVPPAHVRD
                     GQITLNVGTLATHNLVLGNEFIEFQARFGGITETVSVPIGAVSAIYARETGTGMGFEV
                     QPYEPGERPPLAPVEETDEAIVPADDVTSPPVDNGGEAPKRPHLTIVK"
     gene            complement(8637..9248)
                     /locus_tag="VGC69_17910"
     CDS             complement(8637..9248)
                     /locus_tag="VGC69_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0297209.1"
                     /translation="MMVLYSGTTCPFSQRCRFVLFEKGMDFEIRDIDLYNKPEDISVM
                     NPYGQVPILVERDLVLYESNIINEYIDERFPHPQLMPADPVMRARTRLFLYNFEKELF
                     VHVSTLEDRSAKPDDKKMVAARQAIRDRLAQLAPILIKNKYMLGEEFSMLDVAVAPLL
                     WRLDHYGIELPKNAAPIQKYAERIFSRPAYIEALTPSEKVMRR"
     gene            complement(9411..9900)
                     /locus_tag="VGC69_17915"
                     /pseudo
     CDS             complement(9411..9900)
                     /locus_tag="VGC69_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027109.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
     gene            10076..10516
                     /gene="mscL"
                     /locus_tag="VGC69_17920"
     CDS             10076..10516
                     /gene="mscL"
                     /locus_tag="VGC69_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY0297210.1"
                     /translation="MSFIKEFRDFAVKGSVVDLAVGVIIGAAFGKIVDSLVKDIVMPI
                     INFVLGGSVDFTNKFLVLSTPKDYSGPQTYEALSKAGANVLAWGNFVTIIINFILLAL
                     VIFWMVKGVNNLNRKKEAAAAAPAAPVTPEDVALLREIRDLLKK"
     gene            10561..11250
                     /gene="lipB"
                     /locus_tag="VGC69_17925"
     CDS             10561..11250
                     /gene="lipB"
                     /locus_tag="VGC69_17925"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY0297211.1"
                     /translation="MRVRILPSPAPYQETWDAMRAFTEARAADTPDEIWLVEHPPVFT
                     LGLAGKAEHILDAHGIDIVESDRGGQVTYHGPGQVVAYVLLDLRRAGYFVKEYVQRME
                     QAVTALLRELGLPDACAKPGAPGVYVRWPRAWHGPGPSPELAKIAALGIKVRKGCTYH
                     GVALNVAMDLDPFAWINPCGYAGLRTVDLASCGCRIGMEEARERLAQALSRALSPPAQ
                     AGRHLAEVAVE"
     gene            11275..12264
                     /gene="lipA"
                     /locus_tag="VGC69_17930"
     CDS             11275..12264
                     /gene="lipA"
                     /locus_tag="VGC69_17930"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY0297212.1"
                     /translation="MTPMPESPLVAEATTPAPDPHQKQKTKFKVKTIPIQSVPVERLK
                     KPEWIRVKAASPGSRFFEIKRILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKC
                     TRRCPFCDVGHGYPDPLDVDEPLNLARTIAALKLNYVVITSVDRDDLKDGGAAHFVDC
                     IARTRELSPNTRIEILVPDFRGRLDRALAILNAGPPDVMNHNLETVPRLYKQARPGAN
                     YEHSLKLLAEFKALHPEVPTKSGLMLGLGETDEEILQVMRDMRAHDVDMITIGQYLQP
                     SEYHLPVQRYVHPDTFAMFEHEAYEMGFTHAAVGAMVRSSYHADEQAHAAGLA"
     gene            complement(12289..>13362)
                     /locus_tag="VGC69_17935"
     CDS             complement(12289..>13362)
                     /locus_tag="VGC69_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297213.1"
                     /translation="RKKSLIITLALMGVSTTLIGLLPSYHAVGLAAVVLLVALRLLQG
                     VAVGGEWGGAVLIAAEHAPAKWRTFLASAPQYGSPIGLILATGVFRLVSGLPKEEFLG
                     WGWRLPFIVSAVLVLVAFIIRRGVGESPEMQARLRQDRKAHMAPIGLVLRERKRALLL
                     GIGLCLLGISGFYFVTTLMITYTTTYLNITRSQILDVVTWAGLVELISFPIASFIASR
                     IGERRFLVYVTGLCALWSVPMMMLIMTADIGNITLGILMAIFLIGMYYAVLAPYLPKA
                     FPVEMRYTGISLSFQLCGAIFGGTTPLVGLWVAHTFGQNWQPMALMFAIIGAANFLCA
                     IYLPTAERDAARAGNAARPAAQY"
BASE COUNT         2252 a         4077 c         4519 g         2514 t
ORIGIN      
        1 agcttcatcg tggcctattc gatccgccag gacacctgga accgcctgcc cgacgacgtc
       61 aaggcggcca tggatgcggc ctccgaggcc atcgagccgc agatctgcaa acaggtcgac
      121 gactcgcaga cggccaccca gcacaagctg gaagagggcg gcaccgtcta caacacgctg
      181 tcggacccgg tgctcaagga cttgcaggcc aagctgcagg gcgtgggcaa ggtctgggcc
      241 ggggaactgg accagcgcgg caagcccggc acgccggccc tggcggagtt cgagcaactg
      301 gtccacgccc agggggccga atgatgagca gcctggaatc aggaggcgcg ctatccggcg
      361 cccgggcccg cccgggcgcg tcctggatcg cgcggatcag cgaggcctgg cgcgcgatcg
      421 agcgggtctg caccctggtc gcggcacttg ccctgtttgc gaccatgctg ctggtggtgg
      481 ccgatgtgtt catgcgctat gccttgaacc ggccgttcag cttcacctac gacctgatcg
      541 gcctgtacct gctggccgtg gtgttcttct tcacgatctc caacgccttg cgcgaacacg
      601 agcacgtctg cgtggacatc ctgctgcagc gctttcccac ggccggccgg cgcctgtccg
      661 agctggtcac ggcggcgacc ggcctgtcgg tgttctgcct ggtcgggtgg ctggggttcg
      721 agcgcgcgct cgacagctac cggcagggcg atgtgctggc cggcgccatc ccctggccga
      781 cctggctgtc ggtgctgccg gtgcccgtgg gctgcggcgt gctggcgctg cggctggcgc
      841 tgcagttcat cggcaatctg tacagcctgg caacgggagc cgacctgtat cccctgcccc
      901 ccaccgtggc cgagggccgc agcgcccggc tcgaatagga gtgccgcgat ggtgtccggt
      961 ttcatccttt tattgctgtt cctcctgctc gcggtggggg tgccggtggg catctcgatg
     1021 gcgttctcgg gcgccgtcgg cctgtactcg ctgggcggtt tgccggcctt gtcgggcatc
     1081 ttgctgaccg cgccgctgtc gacggtctcg tcgtacgaac tgatcacgat cccgatgttc
     1141 ctgctgatgg ccgacttcgt gctgctgtcg ggcgtggccg acgatctgtt caaggccgcg
     1201 gcggcctggg tcggccgcgt gccgggcggg ctgggcatgg ccacggcgct ggcgggcgcg
     1261 ggcttcggct cgatctgcgg caccagcacg gcctcggccg cgacgctgtc gtccaccagc
     1321 ctgcccgcca tgatccgcca gggctacgat cccaagctcg cggccggcgt ggtggcgatc
     1381 tcgggcacgc tggccatgct gctgccgacc agcgtggcgc tgatcctctt cggcttgctg
     1441 gcccaggtga acatcggcca attgctcatc agcggcatcg tgccggcgat cttcgtcacg
     1501 gccgcgatca tggccaccat cgcctggctg gtctggcgcg acccgtccag cgcgccggcc
     1561 gccctgtcgg tgcgctggag cgagcgcttt cgcctgctgt ggaaagtgag cccgatggtg
     1621 gcgctgttcg ccctggtgac gggcgccatc tacctgggcg tggccacgcc caccgaggct
     1681 tccgccgtgg gggccttcgg cgccatgctg ctggcggcgg ccaagggcaa gctcacgccg
     1741 gccacgctct accagaccgt gctgcgcgcg gcgcacggca cctgcatgat catcatgatc
     1801 ctggtggggg cgtcgatctt cggctacttc ttcacgctca gccacgtgac gcaggacctg
     1861 gtcggctggg tcggggcgct gccggtctcg cgctggatca tcatcgcgct gatcctgatg
     1921 ggctatatcg tgctgggatc gttcatggac caggtggcca tcctggtgct gacggtgccg
     1981 gtggtgctgc cgctcatcaa ggagctgggc ttcgacccca tctggttcgg cgtgatcaag
     2041 atcgtcaccg ccgaagtcgg catgatcacg ccgccggtcg ggttgaactg cttcatcgtc
     2101 gcgcgctatg cccagcggcc ggtgggcgag gtgttccagg gaacgatgcc gcacttcatc
     2161 gcgcatatcg ccgtcatcgc cgtcatggtg gcgttccccg gcatcatcct gtggctgccg
     2221 tccctcatga actgagccgg gtccgccgcc atggatagcg catcttcccc cacgcgggcc
     2281 gggccgctgg ccggcctgcg cgtcgtcgag ttcggcggca tcggcccggc gccgttcgcg
     2341 gccatgctgc tggccgacct gggcgcgcag gtgctgcgca tcgagcgcct gcagccggcg
     2401 gacatcggca tcaagaagcc cgcgcaatac gacttgctgg cgcgcggccg ctcggtgctg
     2461 cgcgtcgatc tcaagcaccc cgacggcgtg gcttgtgcgc aggccatgct ggcgcggtcc
     2521 gatggactca tcgaagggtt ccggccgggc gtgatggaac gcatggggct ctcgccaggg
     2581 gcctgcctgg cgcgcaatcc cggcctggtc tacggccgcg tcaccggctg ggggcaggac
     2641 gggccgctgg cgcaatcggc cgggcacgat ctcaactaca tcgcgctgac gggcgtgctg
     2701 catgcgatcg gacgtcatga tgcgccgccc acgccgccct tgaacctggt gggcgatttc
     2761 ggcggcggcg gcatgctgct ggccttcggc atggtgtgcg ccttgctgga gcgccagcgc
     2821 tcgggccgcg gccaggtggt ggacgcggcc atgatcgacg gcgccgcgac gctgatgacg
     2881 tcgatctacg gcatgatggc cgccggcctg catgccgcgc cgcgcggcgg caacctgctg
     2941 gattccggcg cgccgcacta cgacgtgtac cgttgcgcgg acgggcaata cctcgcggtg
     3001 gcgccgatcg aaaagaagtt tcgcgaggcc ttgttcgggc ggctgggttg ggcgccggac
     3061 gattttcccg atctggacga tgccgcgaac tggccggcaa cgcgcgagcg cctggccgcc
     3121 ttgtttgcga cggatacgca gcgcaactgg tgcgatcgcc tggcgggcgc ggatgcctgc
     3181 gtcacgccgg tgttgtccgc ggcgcaggcg caacggcacc cgcatcacca ggcgcgttcc
     3241 accttcgtcg aaatcgatgg cgtgctgcag ccggcgccgt cgccgcgctt ttcccgcacg
     3301 ccgcccgcgc cgccccaaag cgtgcggcaa atgcctggcg gggcagcggc cctgctgcgg
     3361 cattgggggg tggagagcga ccggatcggc gagtggatcc gggacggcgt ggttgcctag
     3421 atccgccgcg gttccctgca ggggaagggg gggattgcga cctgtcacca aataatatga
     3481 tcgcaatttt agaaacaaaa tttacctttt aaaatgtaaa taaataagta aaaaatggct
     3541 ttttgtttca gattgaacag cgcttcggca tggaaagatg caagctcgcc cggttgtcgg
     3601 gagaggacat ccacaccatg cgcacccttg cccatctact cgttgtcttt gcattatttt
     3661 gcattaattc agtagcttat gccgagagtg gggacatcct gctccgtgtc agagccatcg
     3721 acatgctgcc ccaggtccgt accacgggga ccttgtccac cctcaatgta gggctggata
     3781 ggaagtttct tcctgaaata gatttgtcgt atatgtttac aaatcacata gggacggaac
     3841 tggtgctcgg cacgacccgc agcagcgtca cctcgctgat cggcggcctg ggggagcaaa
     3901 gcctgctgcc gcccaccctg accctgcaat accacttcaa tccggaaggc cggttccgtc
     3961 cgtatgcggg cgcggggatg aactacacgc gtttctacgc gggcagcctg tcgtcccatg
     4021 ggtatccgat cagcatgacc cgggacagct tcggtccggt cctgcagctc ggcatggaca
     4081 tcggcattac caagaacctg ttcttcaaca tcgacgtgaa gaagatctgg atgaagacga
     4141 atctctcgct caacggcatt ccgcgcgtcg gcagcatgcc gctgggcacg atgcatatcg
     4201 atcccatatt cgtcggcgtg gggatcgggt atcgctttta aggggtgggc ggagtacccg
     4261 ctccgctgcc gcctgatcgg catccgccca tgcccggtcc ggctggatcg ccgggcgccg
     4321 caatgataaa aaggccatac attgcggagg cagaggccta tgacataccc gactcgccgc
     4381 agcctgtggg tgctggcctg cctgctcgcg ggccatgccg cgctggccgg cgaagaccgg
     4441 caaaaccgcg ggcacgaccc gtttttccaa atatccaagg ccatcgcgga ctgcccggcg
     4501 cccctggggc cgcttttgac cgagcaggag tggatttccg aagcgcatca ccgcatcgaa
     4561 cgcggcaaca gctgctggcg cgaagggcgc tgccggctgt ccaacggcta tctttacgac
     4621 caggaaatcg ccgagtccgt acagcggcgc ctggatgtca tggaccctgc gacgcattgg
     4681 cgcgaccgca cgacgctctg gctgaccttg cagcggcgct tcatctacgt gcagggctgc
     4741 atgtcgccgg ctttcgacaa gcgcaagttt ctggatgagc ttgcgcagac ggccgacgtg
     4801 gagcgggtga tcgaccagac aacggctggc tcgaaggcgg ctacgatgcc gtacaggacg
     4861 ttgacgccat gacgaagaaa cccccgacct acgtggcgca ttgccgatgc ggcgcggtcg
     4921 aggtcggcgc ttggagcgag cccattgccg tcaacgcttg ctactgcgac gactgccagg
     4981 ccgcggcccg gaccgcctcc aggcgatgag gctgaccgac gcctcgaaga cccggcggat
     5041 gatcgccggt tgcggcggtc ggaagaaaaa gccgaagacg gcgtgcccag ccatcccggc
     5101 tatccgctcg cgctgatcct ccgcatcctg gccgtttggc cgctcatgct gttcagccgg
     5161 ccggtcggcg ctttgccctg aggcaggggc ctaatacggg tgggagagag gactgaaatt
     5221 atggctggaa agccgcatga atacttgttt tatgattttt agacttccaa tataccccca
     5281 taagtacccc caaacttggg cgctggggtt atccagccgg atgcgtcttg accttgtcgt
     5341 ccagcgccag cgaagtccgt agggtgtcgt tgaggcgata ttgctagccg tcaccgctgg
     5401 cacgcaaggc ggctagcacg tcggcatcca ggcgcagttt cactgcttct ctcgtcacag
     5461 ctgctgccgg cagggtggcc acaccgccgg tgggcgacga tctactccgg cgtatgcgcc
     5521 tgggtgaact tgccggcctt ggtttcggcc agcgactcgc gtaacccagg cagcgcttgg
     5581 ccagcgtcgg aggggctatg gtcctatagc aacactagat gttgtgtctc acggtgttgc
     5641 tgccgcaaga acttgtcccg agagagggag ccgtaggggc ttgcttgact tacggcccat
     5701 aacccataaa atttatgggt atggcgacac ttattcagct ccacatgtcc gacgaccact
     5761 ttttgcgcat cgtgcgcgaa gtggcgcaag acacctcaag gatgaagctg agcaagcatg
     5821 ccagggagag gatggcagag cgtggattta ccacgcctca agtctattcc tgcatccggc
     5881 gcggcgctat ccatgagcca gtgcatcagg acattcgggg ggactggaaa tgcacgctca
     5941 gacacgtatg ggccggcgat gaaataaggg ttgctatcgc gcttaagcgc aacagccgcg
     6001 gtgattggat cgcggttctg acggttttct agggagagtt ttatgtacca ctacaccgaa
     6061 tgcggtttgg ataacgtgtg gctggtcaat ggttatcaga tcgaagagat cgacggcgag
     6121 gaatattttt ccattgaaga tgccgatgag ctacaccgag caatagggcg ttctttggct
     6181 gaaaagccgt ctttgacggg ggcagaagtt cgctttttgc gtaaagaatt ggggatgtca
     6241 caacgcgtgt tggcacttct tttgggtacc accgaacaaa ccatgtctct atgggaacgg
     6301 gggtcttccg ttcctgaaag cgagtctcgc ttgttaaagg tcttgtatct agagaaagta
     6361 gatggcaatg tcagcgtgaa tgcccttctg gaacgcatca tagacttgga tagccgagag
     6421 gaagatcgcc tagttttcca ggataccgaa tgcggctgga agatggctgc ctgatcgggt
     6481 cttactagtg attcgtttaa gccgcctccg ggcggctttt tgttggccac tagcgcactt
     6541 gcatggggtg caaggggtct caccctccgc cggtcgtgcc ccaggctaca tgactctact
     6601 caacgtcaga gtcgaggggg actacaccag gcaagatctt gcacaggcag gggaatgagg
     6661 gcggccgccg tttaagtaag gtgcgtcccg ctgctagggc gggctggctt aaacctccag
     6721 taggccgcag cgtgactata gcccagcatt ttggcaagtt gccgcccggt ttccacgaaa
     6781 gcagcttttc ggctcataac ttgctgaagg cctgccccga acctgggtac ccgctttgga
     6841 tgttcaggtc acgcaccatc gctggttgtc tatctcgcca ggctgggggg gcgcgtcagc
     6901 ctggacgccg tagcgcggct ctggaagcgc tccagagccg gctttccccg gcccgggtcg
     6961 cagctccttt ttgtggtcgc aacctgcgac ttggggtgcg acttccacca gactgccggg
     7021 tagccatagc ctggggctat agggggtagc ccagtgcgcg gaaaggtgcg cacttatttt
     7081 cgggggaatc gttgcgaaac tcacccccaa actgccagta accccgcatg gatagcgcag
     7141 gatgaaacca gcctggtgca gctcgctcag caggctgtcc acgttgccat cgtcgtaggg
     7201 cagtacctcg gcgcggatac gcttggggcg gtcttccagg cggccgtcac gatcagccaa
     7261 acactagagg ccggcaaaga gcaatcgggc cagaggtgag cattcggcca gagtctcgtt
     7321 cttgaagagc ccgggtttga gggtgcgcgc gcgggccatt actgctcccc cgggaccagt
     7381 gagtacatac aaggccacgt tgctatggcg atagccctgg tcgtcccaga gggtcacgcg
     7441 ctcggtggcg atgttgtagc cctggcggcg cagctcgatg acgcgggttg ggcattggta
     7501 gcagcccatt ttgcgcagct cgtagctgtt gtgggggcgc agggcgaggg cctgcaggat
     7561 gcgctggcgc tgggcgcccc cagaggtgct tttgtccttg gacatgtcac gcccacgcca
     7621 ggtcggccgc gaggtggcag gcgcgctcgt actcggccgg tgtcgtggca tgtgccgtta
     7681 gggcagcctt cagtgcttcg tcggtgccat actgggggcg cttggccacg ttatcatcca
     7741 gcttggtggt ctgggtcgcg ctcttactgg agtcgcggcc cttttgcttg gtggtggtcg
     7801 acacagttag ccctccatct tcgcagcgca acgcgccaga aaggcgtcaa gctcttcgcg
     7861 catccacacc gtgatggcaa tagaccctct tggactacgt tagacagcaa aaaaccccta
     7921 taggcttgat ttctaggggt ttttagactt ccttggacta cctgatacag gtaagtggtg
     7981 ccggagagag gaatcgaacc cccgaccttc tcattacaag tgagctgctc taccaactga
     8041 gctacaccgg catgtccgtg ccttgcgcga cgaacggcgc gaggcgtgat tgtattgaaa
     8101 acgcgcgggc cgtgcccgcg cgcaatgcgc cgggtctggc gcggtttatt tgacgatggt
     8161 caggtgcggg cgcttgggcg cttcgccgcc gttgtccacg ggagggctgg tgacgtcgtc
     8221 ggctggcacg atggcttcgt cggtttcttc gaccggggcg agcgggggac gctcgcccgg
     8281 ttcgtagggc tgcacctcga aacccatgcc ggtgccggtt tcacgcgcat agatggcgct
     8341 gacggcgccg atcggcaccg aaacggtctc ggtgatgccg ccgaagcggg cctggaattc
     8401 gatgaattcg ttgcccagca ccagattgtg cgtggccagc gtgccgacgt tcaaggtgat
     8461 ctgcccgtcg cgcacgtggg ccgggggcac cagggtgtgc gcatcgacat gcacggtgat
     8521 gtagggcgtg tagccgttgt cggtgcacca ttcgtgcagt gcgcggatca ggtagggttt
     8581 ggtcgaggtt tcgcccatgg cttgcgtgtg tttctgctgc ttgggatgcg gtcggattag
     8641 cggcgcatga ccttttcgga aggcgtgagc gcctcgatgt aggccgggcg cgagaagatg
     8701 cgctcggcgt acttctggat gggcgccgcg ttcttgggca gttcgatgcc atagtggtcc
     8761 aggcgccaca gcagcggggc cacggccacg tcgagcatgg agaactcttc gcccagcatg
     8821 tacttgttct tgatgaggat gggcgcgagc tgggccaggc ggtcgcggat ggcctggcgg
     8881 gcggcgacca tcttcttgtc gtcgggcttg gcgctgcggt cttccagggt ggagacgtgc
     8941 acgaacagct ctttctcgaa gttgtagagg aagaggcggg tgcgggcgcg catgaccggg
     9001 tcggccggca tcaactgcgg atgggggaag cgctcgtcga tgtactcgtt gatgatgttc
     9061 gattcgtaca gcaccaggtc acgctcgacc aggatgggca cctggccgta ggggttcatg
     9121 accgagatgt cttccggctt gttgtacagg tcgatgtccc ggatctcgaa atccatgcct
     9181 ttttcgaaga gcacgaagcg gcaacgttgc gaaaaagggc aggtcgtgcc ggagtagagc
     9241 accatcatga cgggtaagtc cttgtattta aaagctaaag ccggcgctca ttataaaaat
     9301 ggggagactg gcgcgtggcc gattatggaa acagtgtagc tttttttcga cggacatgtt
     9361 tccagccggc cggccggtgg gctgtttgcg gggtcttgtg cctggcggtc ctagtcgatg
     9421 ccgatgacga ttgtggtgtc gtccaggtat tggtgcggcg gtatttccag gttgtcgagg
     9481 gccggtttgt cctggaagac gcggccggcc aggtcgaagg tggagccgtg gcaggggcag
     9541 aggaatccgc ctttccagcc ggcgggcatg ccgtcggcgg cgccggtttc gaaatgcggg
     9601 atgggcgagc agcccatgtg ggtgcccagg ccgatgcaga cgaagacccc gggcttgcgc
     9661 gagcggtatg cgttgcgggc gtagccgggg gtgtagaagg ggcgctgcga ttgcgggtcg
     9721 gccagctggc tgttgcaacg gggcgccgat cagggggacg gccaggccca ggccggtggc
     9781 gccgcccagg gcgcaggccg tggcaaccag ggcacgccgg gcggggtcgg ggggaagcgt
     9841 cgaaggcgcc gtgtcggcgt cgttgcgcgg gcatgccggg accggagagt cggggctcat
     9901 gatgcctcct ggagccctgg tctgcgcccc gttgcaacag ccagctgccg ggtggttgcg
     9961 gacgctggtg gcgatttgta acaaccgcac ggccgcatct gcgtattata gccaccggcc
    10021 tgttttaggc cgcatttccg acttgagtcc cgggcgtaga tcccaaggag tgaaaatgag
    10081 ctttatcaaa gagtttcgcg actttgcagt caagggcagt gtcgtcgact tggccgtcgg
    10141 tgtgatcatc ggcgcggcct tcggcaagat cgtggattcg ttggtcaaag acatcgtcat
    10201 gccgatcatc aacttcgtgc tgggcggctc ggtggacttc accaacaaat tcctggtgct
    10261 ttccacgccc aaggactata gcggcccgca aacgtacgag gcgctctcca aggccggcgc
    10321 caacgtgctg gcatggggca atttcgtcac catcatcatc aacttcatcc tgctggccct
    10381 ggtgatcttc tggatggtga agggcgtgaa caacctgaac cgcaagaagg aagcggccgc
    10441 ggccgcgccg gcggctcccg tcacgcccga agacgtggcg ctgctgcgcg aaatccgcga
    10501 cctgctgaag aagtgagcca cgggaggcgg tccgccttcc catcgatgcg ttgagatgtg
    10561 atgcgggtcc gtatcctgcc gtccccggcg ccctaccagg aaacctggga cgccatgcgc
    10621 gccttcaccg aggcgcgcgc ggccgatacg cccgacgaga tctggctggt cgagcatccg
    10681 ccggtcttca cgctgggcct ggcgggcaag gccgagcata tcctggacgc gcacggcatc
    10741 gacatcgtcg agtccgaccg cggcggccag gtcacctatc acggacccgg gcaggtcgtg
    10801 gcctatgtgc tgctggacct gcgccgggcc ggctatttcg tcaaagaata cgtgcagcgc
    10861 atggagcagg ccgtgacggc gctgttgcgc gagctgggcc tgccggatgc gtgcgccaag
    10921 cccggcgcgc cgggcgtcta cgtgcgctgg ccgcgcgcat ggcatgggcc cggcccgtcg
    10981 ccggagttgg ccaagatcgc cgcgctgggc atcaaggtgc gcaagggctg cacctatcat
    11041 ggcgtggcgc tcaacgtggc catggacctg gacccctttg cctggatcaa tccttgcggc
    11101 tatgcgggcc tgcgcacggt cgacctggct tcgtgcggct gccgcatcgg catggaagag
    11161 gcgcgcgagc gcctggccca ggcgttgtcg cgcgcgttgt cgccgcctgc gcaggccggt
    11221 cggcacctgg ccgaggtggc agtagaatga gcccggacga acggaaaccc tgcaatgacc
    11281 cctatgcccg aatcccccct tgttgccgag gcaacgacgc cggctcctga tccgcatcag
    11341 aagcaaaaga ccaaattcaa ggtcaagacc attcccatcc agtcggtgcc ggtcgaacgc
    11401 ctgaaaaaac ccgagtggat ccgcgtgaag gcggcctcgc ccggttcgcg cttcttcgag
    11461 atcaagcgca tcctgcgcga gcacaatctg cacacggtgt gcgaggaagc gtcgtgcccg
    11521 aacatcggcg aatgcttcgg caagggcacg gccaccttca tgatcatggg cgacaagtgc
    11581 acgcggcgct gcccgttctg cgacgtgggc cacggctatc ccgacccgct ggacgtggat
    11641 gagccgctca atctggcgcg cactatcgcc gcgctcaagc tcaattacgt ggtgatcacc
    11701 tcggtcgacc gcgacgacct gaaggacggc ggggcggcgc acttcgtcga ctgtatcgcg
    11761 cgcacgcgcg agctgtcgcc gaacacgcgc atcgagatcc tggtgccgga cttccgcggg
    11821 cggctggacc gcgcgctggc catcctcaat gccgggccgc cggacgtgat gaaccacaac
    11881 ctggaaaccg tgccgcgtct gtacaagcag gcccggccgg gcgccaacta cgagcattcg
    11941 ttgaagctgc tggccgagtt caaggccctg cacccggagg tgcccaccaa gtcgggcctg
    12001 atgctgggcc tgggcgagac ggacgaggag atcctgcagg tgatgcgcga tatgcgcgcg
    12061 cacgatgtgg acatgatcac catcggccag tacctgcagc cctccgaata ccacctgccg
    12121 gtgcagcgct acgtgcatcc cgataccttc gcgatgttcg agcacgaagc ctacgagatg
    12181 ggtttcacgc acgcggccgt gggggcgatg gtacggtctt cgtaccatgc cgatgaacag
    12241 gcgcacgcgg ccgggttggc gtagcgtcgg ctgcccccag ggcggcagtc agtattgcgc
    12301 ggccggcctg gcggcattgc cggcgcgcgc cgcgtcgcgt tcggcggtgg gcaggtagat
    12361 cgcgcacagg aagttggccg cgccgatgat ggcgaacatc agggccatgg gctgccagtt
    12421 ctggccgaag gtgtgcgcca cccacaggcc caccagcggc gtggtgccgc cgaagatcgc
    12481 gccgcatagc tggaagctca gcgaaatgcc ggtatagcgc atttccaccg ggaaggcctt
    12541 gggcaggtag ggcgccagca cggcgtagta catgccgatc aggaagatcg ccatcaggat
    12601 gccgagggtg atgttgccga tgtcggccgt catgatcagc atcatcatgg gcaccgacca
    12661 cagcgcgcac aggcccgtga cgtagaccag gaagcggcgc tcgccgatgc gcgaggcgat
    12721 gaacgacgcg atcgggaagc tgatgagctc gaccaggccc gcccaggtca ccacgtcgag
    12781 gatctggctg cgcgtgatgt tcaggtaggt ggtggtgtag gtgatcatca gcgtggtcac
    12841 gaagtagaag cccgagatcc ccagcaggca caggccgatg cccagcagca gcgcgcgctt
    12901 gcgttcgcgc agcaccaggc cgatgggggc catgtgcgcc ttgcggtcct ggcgcaggcg
    12961 cgcctgcatt tcggggctct cgccgacgcc gcggcggatg atgaaggcca ccagcaccag
    13021 cacggcgctc acgatgaagg gcaggcgcca tccccagccc aggaattctt ccttgggcag
    13081 gcccgagacc aggcgaaaca cgcccgtggc gaggatcagc ccgatggggc tgccgtactg
    13141 cggcgcggag gccaggaagg tgcgccactt ggccggcgcg tgctcggcgg cgatcaggac
    13201 ggcgccgccc cactcgccgc ccacggcgac gccctgcagc aggcgtagcg cgaccagcag
    13261 cacgacggcg gccaggccca cggcgtggta ggagggcagc aggccgatca gcgtggtgga
    13321 cacgcccatc agcgccagtg tgatgatgag gctcttcttg cg
//
LOCUS       DASWPO010000338         6850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1152605, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000338 DASWPO010000000
VERSION     DASWPO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152605"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1251)
                     /locus_tag="VGC69_17940"
     CDS             complement(91..1251)
                     /locus_tag="VGC69_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297214.1"
                     /translation="MNVRIKLLAGSIALALSAAGHAADPIKIGVSGHYTGGSSSMGVS
                     MRDGVRLAIEEINQSGGLLGRQLVSIERDDEAKNERGVQIAQELINREHVTATVGYAN
                     TGVALASQRFYQDAEIPVFNNVATGTVITEQFKAPDYADNYVFRNAAKDSIQAPMLVE
                     EAVTKRGYKKPAILADSTNYGQLGREDLEKALAAKGIKAVAEEKFNIGDVDMTAQLLK
                     AKEAGADVILTYGIGPELAQIANGMAKLDWKRPIIGSWTLSMSNYIDNSGANGEGATM
                     PQTFIQAPTTPKRKAFIDAYLAKFKPKNDRIESPVSAAQGYDSVYLLAAAIKQAGTTD
                     GAKVRAALENLQAPYEGVVMTYDKPFTHDNHDAIDASKVVMGVVKGGRVVAE"
     gene            1535..2059
                     /locus_tag="VGC69_17945"
     CDS             1535..2059
                     /locus_tag="VGC69_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY0297215.1"
                     /translation="MKVRSLTLAAMFGLAAACTSAWADDVRAGALDIKEPWARATVPG
                     QANGAGYLEVDNTGTAPDRLVAVRSDAAEKVEVHTMTMDGGTAQMRQVEGGLEVPAGG
                     KATLSPGGNHLMFIKLKAPFAAGSSVPVVLTFEKAGEVAVTLPVRPLGGMSHDTGNMN
                     HGGMSGMSMPGMKN"
     gene            complement(2078..2902)
                     /locus_tag="VGC69_17950"
     CDS             complement(2078..2902)
                     /locus_tag="VGC69_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0297216.1"
                     /translation="MTNASGDDTPAGVQTIRRAVVILRLVACGQERGVRLIDLVRMSG
                     LNRPTVHRLLKTLVQEGAIEQEPETRRYLIGPEISLLGLARTRRFPLLTLAAPYLAEL
                     ADQVGDTVFLSIRHGHDSICIGRRTGHHPIQVLSIEVGVRRPLGIGVSGIALLASLPG
                     KESSALVAANAARLAVLGENPEDILARVAKARATGIAHAPAGLMPGTSAVAIPIPGPN
                     GAALGAVTITAMAERLAPDRFTEVIGRMRITAQSIAQRQAEITSAVSKPVISARGR"
     gene            complement(2899..3516)
                     /locus_tag="VGC69_17955"
     CDS             complement(2899..3516)
                     /locus_tag="VGC69_17955"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0297217.1"
                     /translation="MRITAPELTPEQTYKLLSGIVVPRPIAWISTLGPGGKVNLAPFS
                     CFTFVSNTPPMVGVNIGRKAGLRKDTARNILAHRHFVVNIGDETLLDAIHSSAEEHPP
                     EVSEAELLGLDVLPGEHIDTPRLAAAPISMECRLHSVTPFGDTGAEFFVGEVLVFHIR
                     DGLARDGKIDTERLRPVCRIGGPNYASLGPIVSKRVIRQTPKEVI"
     gene            3765..4736
                     /locus_tag="VGC69_17960"
     CDS             3765..4736
                     /locus_tag="VGC69_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297218.1"
                     /translation="MTRYMLARACAAVALAGLTPYAQAAWPDKPIRIIVPYPPGGNVD
                     VAARLISPGLQAELGQSFIIENKPGAGGMIAGEYVAHSEPDGYTLFMAANGPLLFSPL
                     IFKRQAYKWDRDFAPISSISYTPLVLEVKPALPVKTVGELIALARKEPGKLTMASPGA
                     GTTNHLVSEMLQHVAGVRWITAQYKGNAPANTDLLGGQVDFQFDQISVALPFIKDNRL
                     RALAVTTAKRMPSMPDVPTFAEAGVQGMEASTFTGLLAPKGTPPEILAKLSRALADIL
                     KQPEIIKRFDELGAQARGSTPEEFTRYLADEDARWTPIIERAGITAN"
     gene            4775..5170
                     /locus_tag="VGC69_17965"
     CDS             4775..5170
                     /locus_tag="VGC69_17965"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0297219.1"
                     /translation="MTRRSIYAEGFSHKNPIPAACRIGNMLYSGSIQGTDLVTGAYGG
                     TIERQCELMFDHVRRIAAAGGGSLADIVKMTVWMRDRDQRAALNTVWLRAFPNPEDRP
                     ARHTMKADLDGDKLIECDFIAVLAQGGEA"
     gene            5167..6039
                     /locus_tag="VGC69_17970"
     CDS             5167..6039
                     /locus_tag="VGC69_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0297220.1"
                     /translation="MTQYQSFQAHPTAPRARLPAGSCDSQFHVFGPAGRYPVRPGAAY
                     EMPGATIEVALRLHRLLGIERGVIVQATTYGADHQIVLDGLAAAGPDYRGCANAAVLA
                     ERDDAYIARLHDAGVRGARYTRQGLGISMEPAAFQRSIARLRELGWYAKFQPEPDGIL
                     AQAAQFENLDIPVLIDHMGRADPAAGERDPSRRKLEELLKRGNFWVMLSLTEKLSRTG
                     QPWDDVVPLAQALVAANPDRVVWGSDWPHPVSTKATPDEGRLVDQLARYAGDAAILRK
                     ILVDNPVRLFGFDK"
     gene            6036..>6850
                     /locus_tag="VGC69_17975"
     CDS             6036..>6850
                     /locus_tag="VGC69_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0297221.1"
                     /translation="MKLLSFEHRGRSSFGLLRGDADIVDLGGLGYDSLRAALAAQALP
                     ELARRGAVLPATLRLAAVRLLPPIPVPEKIICVGVNYGNRNEEYKDGSAPPAYPSVFP
                     RFPGSFVGHGQPLLRPPESEQLDYEGEIALIIGRRGRRIAAADAWSHVAGLTCANEGT
                     VRDWLRHGKFNVTQGKNFDASGSMGPWMVSADAFDPAAPLTVTTRVNGERRQHDTTGN
                     LMFPFAELIRYISIWTTLEPGDVISTGTPVGAGVRFTPPRFLKPGDVVEVEVPG"
BASE COUNT         1121 a         2183 c         2309 g         1237 t
ORIGIN      
        1 cagcccccag tgggggcgag catcggaatt ccgcagggca gcctcggcgt ctcaagaacc
       61 cagacagcgc agcggcttga gaacgtccag ctactcggca accacccgcc cacccttgac
      121 cacgcccatc acgaccttgg acgcatcgat ggcgtcgtgg ttgtcatgcg tgaaaggctt
      181 gtcgtaggtc atcaccaccc cttcgtacgg ggcctgcagg ttttccagcg cggcgcgcac
      241 cttggccccg tcggtcgtgc cggcctgctt gatggcggcg gccagcagat agaccgagtc
      301 gtagccctgg gcagccgata cgggcgattc gatgcggtcg ttcttgggct tgaacttcgc
      361 caggtaggcg tcgatgaacg ccttgcgctt gggcgtggtc ggcgcctgga tgaaagtctg
      421 cggcatggtc gcgccttcgc cgttggcgcc cgagttgtcg atgtagttgg acatcgacaa
      481 cgtccagctg ccgatgatgg gccgcttcca gtcgagcttg gccatgccgt tggcgatctg
      541 cgccagttcg ggaccgatgc cgtaggtgag gatcacgtcg gcgccggctt ccttggcctt
      601 gagcagttgc gcggtcatgt ccacgtcgcc gatgttgaat ttctcttcgg ccacggcctt
      661 gatgcccttg gcggccaggg ctttttccag gtcttcacgg ccgagctgac cgtagttggt
      721 ggagtccgcc aggatcgccg gttttttgta gccgcgtttc gtgacggctt cttccacgag
      781 catcggcgct tggatgctgt ccttggcggc gttgcggaac acgtagttgt cggcgtagtc
      841 gggcgccttg aactgctcgg tgatcaccgt gccggtggcc acattgttga aaacggggat
      901 ttccgcgtcc tggtagaaac gctgcgaggc cagcgccacg ccggtattgg cgtagcccac
      961 cgtggccgtc acgtgctcgc ggttgatgag ctcctgggcg atctgcacgc cgcgttcgtt
     1021 cttggcctcg tcgtcgcgct cgatgctgac gagctggcgg cccagcaggc cgccgctctg
     1081 gttgatttcc tcgatggcca ggcgcacgcc gtcgcgcatg ctgacgccca tcgaggagga
     1141 gccgccggtg tagtgacccg agacaccgat cttgatcgga tcggcggcgt ggccggcggc
     1201 ggataaagca agggcaatac tgcccgcaag cagtttgata cgcacgttca tggtgcttgt
     1261 ctccgtttgt tatggaaaag ctgctgcgca aggccccggg catctggacg cgccctgtga
     1321 tgacttgccg ccgcaatccg gaaggtactt tcggattgct gtcgttactg tctgcgatgt
     1381 tgcttacttg agtctgaata ttggacatgg ggttttccga gtccgtgtaa tcccttggtc
     1441 tgtaaggtgg acctcgcgcc gccggaatgc cggcgcgggc cggggtggct acactggcgc
     1501 atcacgctgt ttgcatcgat gcaggaggag cgccatgaaa gttcgatcac tcacgctggc
     1561 cgccatgttc ggcctggccg cggcctgcac gtcggcctgg gcggacgatg tccgggcggg
     1621 cgcgctggac atcaaggagc cctgggcacg cgccacggtg ccgggacagg ccaacggcgc
     1681 gggctacctg gaggtggaca acacgggcac ggcgcccgac cgcctggtgg cggtgcgctc
     1741 ggacgcggcc gagaaagtgg aagtgcacac catgaccatg gatggcggca cggcgcagat
     1801 gcggcaggtc gaaggcgggc tggaggtgcc ggcgggcggc aaggcgacgc tgtcgcccgg
     1861 cggcaatcac ctgatgttca tcaagctgaa ggcgccgttc gcggcgggaa gctcagtgcc
     1921 ggtggtgctg actttcgaga aggcgggcga ggtggcggtg acgctgccgg tgcggccgct
     1981 gggcgggatg agccacgaca cggggaatat gaaccatggc gggatgtccg gaatgagcat
     2041 gccggggatg aagaactgat tgccgttgcg tgcccgctca gcgcccgcgc gcggagataa
     2101 caggtttcga aactgccgac gtgatctcgg cctgtcgttg cgcaatcgat tgggcggtga
     2161 tgcgcattct tccgatgact tcggtgaaac gatctggcgc gagtctttcg gccattgcgg
     2221 tgatcgtgac ggcgccgagg gcggcgccgt tgggcccggg tatgggaatg gcgacggcgc
     2281 tggtgcccgg catcagtccg gcgggcgcgt gggcgatgcc ggtggctcgt gccttcgcca
     2341 cgcgggccag gatgtcttcc ggattttcgc ccagcaccgc caggcgggcc gcgttggccg
     2401 caaccaaggc cgagctttct ttgccaggca ggctggcgag cagcgctatg ccggatacgc
     2461 ctatgcccag cgggcggcgt acgccgactt cgatcgacag cacctggatg gggtgatggc
     2521 cggtgcgccg gccgatgcag atcgagtcgt ggccatggcg gatgcttagg aacacggtgt
     2581 cgccgacttg atccgcgagt tcagccaggt agggggcggc caaggtaagc agcgggaaac
     2641 ggcgcgtccg ggccaagccc agcaggctga tttccgggcc gatcaggtag cgccgcgttt
     2701 ccggctcttg ttcaatggcg ccttcctgga cgagtgtctt cagcaatcgg tgcaccgtcg
     2761 gtcggttcag gccggacatg cgtaccaggt cgatcagccg gacgccgcgc tcctggccgc
     2821 aagccaccag gcgcagaatg accaccgcgc ggcggatggt ctgtaccccc gccggcgtgt
     2881 cgtcgccgga tgcattggtc aaatgacctc cttgggtgtt tgccggatca cccgtttgga
     2941 gacgatgggc cccagcgacg catagttggg gccgccgatg cggcagaccg gacgcagccg
     3001 ttccgtgtcg atcttgccgt cgcgcgccag tccgtcgcgg atgtggaaga ccagcacttc
     3061 gccgacgaag aattccgcgc cggtgtcgcc aaagggcgtg acgctgtgga gccggcattc
     3121 catgctgatc ggcgcagccg ccaaacgtgg cgtgtcgatg tgttcgccgg gcaggacgtc
     3181 cagccccagc agctccgcct cgctgacctc ggggggatgc tcctcggcgc tggaatggat
     3241 ggcgtcgagc agggtctcgt cgccgatgtt caccacgaaa tgacggtgcg ccaggatgtt
     3301 gcgcgcggtg tccttgcgca ggcccgcctt gcggccgatg ttcacgccga ccatgggcgg
     3361 cgtgttcgag acgaaggtga aacagctgaa gggcgccagg ttgaccttgc cgcccggccc
     3421 cagcgtgctg atccaggcga tgggacgcgg caccacgatg ccgctcagga gtttgtaggt
     3481 ctgttcgggg gtaagttcag gtgcggtgat gcgcatggcg gactcgaatt caggcatccg
     3541 gatattggac cattgatcgg tggatcgcac gcatgacttt tgcgcgccga ggcctatttt
     3601 cagtgttttc ctgatggcat aggtaaagct gcctgacgga cattctcgat ttaagtccgg
     3661 attccggact ttttcggaat ttttacgttg tcgccatcgg cgcaagtttc tagagtctct
     3721 tcacacaaga tcgagaaacc cgacgacacg tggagacaac aatcatgacc cgctacatgc
     3781 tggccagagc ctgcgcggct gtcgcgctgg ctggcctgac cccatacgcg caagctgcct
     3841 ggcccgacaa accgatcagg atcatcgtgc cttatcctcc gggcgggaac gtggacgtgg
     3901 ccgccaggtt gatcagcccc ggcctgcagg cggaactggg gcagtccttc atcatcgaga
     3961 acaagcccgg cgcgggcggc atgattgccg gcgaatacgt cgcacactcc gagcccgacg
     4021 gctatacgct gttcatggcc gccaacgggc cgctcctgtt cagcccgctg atcttcaagc
     4081 gccaagccta caagtgggat cgcgattttg cgccgatcag ctcgatctcc tatacgccgc
     4141 tggtgctgga ggtcaagccg gccctgccgg tcaagaccgt tggcgaactc attgccctgg
     4201 cgcgcaagga gcccggcaag ctcaccatgg catcgccggg cgcgggcacg accaatcacc
     4261 tggtcagcga gatgctgcag cacgtggccg gcgtgcgctg gattaccgcg caatacaagg
     4321 gcaatgcgcc tgccaacacc gatctgctgg gcggccaggt ggattttcag ttcgaccaga
     4381 tttcggtggc cttgcctttc atcaaggaca accgcctgcg ggcgctggcc gtgaccacgg
     4441 ccaagcgcat gccgtcgatg ccggatgtcc ccacgttcgc cgaggccggc gtgcaaggca
     4501 tggaggcgtc gaccttcacc ggcctgctcg ctcccaaggg cacgccgccg gagatcctgg
     4561 cgaagctgag ccgggcgctg gccgacattt tgaagcagcc ggagatcatc aagcgctttg
     4621 acgagctggg tgcgcaggcg cgcggcagca cgccagagga attcacccgc tacctggccg
     4681 acgaggatgc ccgctggacg cccatcatcg agcgcgccgg catcaccgcc aattgagtgt
     4741 tccccgcgca ctgcgcgcta cccaggactc cgacatgaca agacgcagca tctacgccga
     4801 aggtttcagc cacaagaacc ccattcctgc cgcttgccgc attggcaaca tgctctattc
     4861 gggcagcatc cagggtaccg atctcgtcac gggcgcctat ggcgggacga tagagcgcca
     4921 atgcgaactg atgttcgacc acgtgcgtcg cattgccgca gccggcggcg gctcgttggc
     4981 cgacatcgtc aagatgaccg tctggatgcg cgatcgcgat cagcgcgccg cgctcaatac
     5041 ggtgtggcta cgcgccttcc cgaaccccga ggaccgcccg gcgcgccaca ccatgaaggc
     5101 cgacctggac ggcgacaagc tcatcgagtg cgacttcatc gcagtgctgg cccagggagg
     5161 cgaagcatga cgcaatacca atctttccag gctcatccca ccgcgccgcg cgcgcgcctg
     5221 cctgccggct cctgcgacag ccagttccat gtcttcggac ccgccggccg ttatccggtg
     5281 cgtcccggcg cggcgtatga aatgcccggc gccaccatcg aggtcgcctt gcgcctgcac
     5341 cggctgctgg gcatcgaacg cggcgtcatc gtgcaggcga ccacgtatgg cgccgatcac
     5401 cagatcgtcc tcgacgggct ggccgccgcc ggcccggact atcgcggttg cgccaatgcg
     5461 gccgtgctgg ccgagcgcga cgatgcctac atcgccaggc tgcacgatgc cggcgtgcgc
     5521 ggagcgcgct atacccgcca ggggctgggc atcagcatgg agccggcggc gttccagcgc
     5581 tccatcgcgc gcctgcggga actgggctgg tacgccaagt ttcagccgga gccagacggc
     5641 atcctggcgc aggccgcgca gttcgagaac ctggacatcc ctgtgctgat cgaccatatg
     5701 ggccgcgccg atccggccgc gggcgagcgc gaccccagcc ggcgcaagct ggaggaactg
     5761 ctcaagcgcg gcaacttctg ggtgatgctg tccctgacgg aaaaactatc gaggaccggc
     5821 caaccctggg acgacgtggt gccgctcgcg caggcgctgg tcgcggccaa tcccgatcgc
     5881 gtggtctggg gcagcgattg gccccatccg gtctcgacca aggcgacgcc cgacgaaggc
     5941 cgcctggtgg accagctggc gcgctacgcg ggcgatgccg ccatcctgcg aaagatcctg
     6001 gtcgacaacc ccgtccggct ttttggattc gacaaatgaa gctgctttct ttcgaacacc
     6061 ggggccgatc aagcttcggc ctgctgcgcg gcgacgccga tatcgtcgac ctgggcggcc
     6121 tgggctacga cagcttgcgg gccgctctcg cggcacaggc gctgcccgag ttggcgcgtc
     6181 gcggcgccgt cctgccggcc acgttgcggc tggcagcggt gcgcctgctg ccgccgattc
     6241 ccgtgccgga gaagatcatc tgcgtcggcg tgaattacgg caatcgcaac gaggaataca
     6301 aggacggcag cgcgccgccg gcctatccca gcgtatttcc gcggtttcct ggctcctttg
     6361 tcggccatgg ccagcccttg ctgcgtccgc ccgagtccga acaattggac tacgagggcg
     6421 agatcgccct catcatcggc aggcgcgggc ggcgcatcgc ggcagccgat gcctggtccc
     6481 acgtcgccgg cctgacctgc gccaacgagg gcacggtgcg cgactggctg cgccacggca
     6541 agttcaacgt gacgcagggc aagaacttcg atgcgagcgg ctcgatgggg ccgtggatgg
     6601 tgtcggccga tgctttcgat ccggcagcgc cgctgacggt caccacccgc gtcaacggcg
     6661 agcggcggca gcacgacacg accggcaatc tcatgttccc attcgccgaa ttgatccgct
     6721 acatctcgat ctggaccacg ctggaacccg gcgacgtcat ttccaccggc acgccggtgg
     6781 gcgcgggcgt gcgcttcacg ccgccgcgct ttctcaagcc cggcgacgtg gtggaagtgg
     6841 aggtgcccgg
//
LOCUS       DASWPO010000339         8009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1152643, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000339 DASWPO010000000
VERSION     DASWPO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152643"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1558)
                     /locus_tag="VGC69_17980"
     CDS             complement(143..1558)
                     /locus_tag="VGC69_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEY0297222.1"
                     /translation="MNVRALEILLAGQPAGILFQYGEQGEDPVIRFEASEAFAARPDA
                     PVLSLSMLAPTPDAQRALWLNRTAPIFNGQPAGHGNTWLPAFFQGLLPEGVFREHLAQ
                     LRGCDPHDHLELLAAGGLDLPGNLTARPIELTPRDMTHLVTQDADALEMSVMQAPLDT
                     AMSISGVQPKIGVTEKDGRYVARTRLGDTRIIAKLPTVGYPLLPEVEHLSLLLAQAAG
                     VDTCEAKLEPLEKLEAEHGYDLGDDVHAQTRFLAVERFDRHADGSRTHVEDFGQILGM
                     VPARKYSRSYLEIAALLMAPPKAYAGQPNRMGEPAVHELLRRLVVNELLGNVDMHLKN
                     IGVIYRDGRTPELSPAYDIVAYAAYGMRQGHALHLLPPDPQAPKARLKAGEPARAGRP
                     QVTPALLRTFCAKLDIPEKPAATAIAGAVKLAVRHWPDLIAASALTQRQKSNLSTHVE
                     AHPMVASLRRRGHAAPTSAPG"
     gene            complement(1555..1836)
                     /locus_tag="VGC69_17985"
     CDS             complement(1555..1836)
                     /locus_tag="VGC69_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0297223.1"
                     /translation="MTSIPELAATLAAERRKAKIAHQALQKLTGLSALGLRRVLSGEH
                     DFKVSTLLSVADRLGLEVVLVPKKAAQALQAEQAPAMQTGVDAALERLK"
     gene            complement(1963..2577)
                     /locus_tag="VGC69_17990"
     CDS             complement(1963..2577)
                     /locus_tag="VGC69_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0297224.1"
                     /translation="MKLYYLPGACSLTIHIALEWIGKPFEAQKMERSALKTPEYFKIN
                     PVGSVPALSDGDFTLTQNVAIVSYLAAKYPEAGLLGDGTPKSQAETLRWLAFINSDIH
                     RTFSRIFGAQRFVSSEVAQEELRSQAAQTLRDYFTIIDTQLTGRDYLTGAKPTAADAY
                     LFVVLRWAVATHIDLSGLAQLPKFVERMQANPGVAAALKDEELA"
     gene            complement(2622..3002)
                     /locus_tag="VGC69_17995"
     CDS             complement(2622..3002)
                     /locus_tag="VGC69_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY0297225.1"
                     /translation="MTERQLTVLAALNMIFAVAAGAFGAHGLKRMVDAELLGIWQTGV
                     QYHLIHALGLFVVAWLCSRYPAAGLLPAAGVAMFAGIVLFSGSLYVLTLTGARWLGAI
                     TPLGGVAFLLAWAMTAWAVWKAPA"
     gene            3108..3911
                     /locus_tag="VGC69_18000"
     CDS             3108..3911
                     /locus_tag="VGC69_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0297226.1"
                     /translation="MFLRLGRRALAAALMMAAAGLAQAAEPLRIGVIASVANEATEIA
                     IAQAKKQGLDVKLVEFSDWVMPNVAVADGSIDANFFQHEPFLDLFNKSRDTHLMPIAY
                     GYSTTIGVFSKKLKPGDAIPRGATLGVPSDPVNTGRALLLLQSMGVLKLRAGTGNMAA
                     TTDVVDNPRQVKLVSIEGAQSARVFDDVTASVTYTTFAKQAGIDEKDGLAFDNTDPEV
                     VKHYAIRWVARPDKANDPRLLQFIKIYQSSPEVKATLRRLYGSLIDFPW"
     gene            3935..5632
                     /gene="ybaL"
                     /locus_tag="VGC69_18005"
     CDS             3935..5632
                     /gene="ybaL"
                     /locus_tag="VGC69_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEY0297227.1"
                     /translation="MEHSVPLITTLAAGFGMALILGFVAERLKVPALVGYLIAGIIIG
                     PATPGFVADVQLASELSEIGVMLLMFGVGLHFSLQDLLDVKHLAVPGAVVQMALATAL
                     GMLLAWWWGWSWGGGLIFGLSLSCASTVVLLKGLEARGVLETLNGRIAVGWLVVEDLA
                     TVLVLVLLPPLAGVLGGHVPQHGVVDAPLWLTIGKTLLQVAVFIGLMLVVGRRALPWL
                     LWQVSRTGSRELFTLCVIAVAIGIAYGAAQLFSVSFALGAFFAGMVMRESEFSHRAAE
                     ESLPLRDAFSVLFFVSVGMLVDPAILVRQPLHVLGVLLIIMFGKSLAAMALVLVFRYP
                     LNTALIVSASLAQIGEFSFILAGLGVSLGLLPSEGMSLVLAGALISIALNPVLFAAIE
                     PLRRWILARSALARRLEARGDPYAELPPSTERKYLEGQVVLVGYGRVGRRIAAELVAH
                     AIPFVVAEQNRERVEELRKQGMAAVSGDAAEPAVLIQAHIAHAAMLVVAAPDPVDVRK
                     MVDTARKLNPGIEIVLRTHSEDESHLLRKENLGAVFFGEEELARGMAGHVLARFAPH"
     gene            complement(5636..6496)
                     /gene="asd"
                     /locus_tag="VGC69_18010"
     CDS             complement(5636..6496)
                     /gene="asd"
                     /locus_tag="VGC69_18010"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394848.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEY0297228.1"
                     /translation="MSFNDSLFLACQRLAPHHLISRLAGSLADSRAPWLKNVLIGRFA
                     RRYGVAMQEARVEDPLAYESFNAFFTRALKPGARPLDATPGAVLCPADGSVSQAGRIV
                     DGRIFQAKGHDFGLAALLGGDAARAAPFEGGSFATIYLSPRDYHRVHMPLPGTLREMI
                     YVPGKLYSVNPLTARNVPELFARNERVACIFDTPGHGPMALVLVGAMIVASVETVWAG
                     RIAPHRSGVVATRYEGGQAARLAQGAEMGRFLLGSTVVALFGPGRVDWAELAAPGNAV
                     RMGQRLGHAL"
     gene            complement(6711..7169)
                     /locus_tag="VGC69_18015"
                     /pseudo
     CDS             complement(6711..7169)
                     /locus_tag="VGC69_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7320..>8009)
                     /locus_tag="VGC69_18020"
     CDS             complement(7320..>8009)
                     /locus_tag="VGC69_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase family dipeptidase"
                     /protein_id="HEY0297229.1"
                     /translation="IQLAHAIATLVSPTGQVLVPEWRPKELPASVRRVLADCEVDGGA
                     DGPVIEPGWGEPGLTPAEKVYGWNSFEVLAYKTGNPDTPVNAIPPRAWARCQLRFVVG
                     LDTEDLLPALRRHLDRHGFPMVKIATTRETLFKATRIDPDNPWVQWAVQSLEQTTGKK
                     PAILPNLGGSLPNDIFTDVLGLCTVWIPHSYPGCSQHAPNEHLPPELLREALCLMAGV
                     YWDMGSGNTPH"
BASE COUNT         1199 a         2468 c         2919 g         1423 t
ORIGIN      
        1 agtcgacgta cgaacgtcag tcgggcatgc ttatgcgtgt tcatccggcc gggctccttg
       61 agtgaactgg gggtttggcg atttccagtt tctcaaatcc ggttcggatg aaccatgcat
      121 acaacctatt gaatcttcac acctagcccg gggcgctggt cggggcggcg tgtccgcgcc
      181 ggcgcaggga ggccaccatg ggatgggcct cgacgtgcgt cgataagttg ctcttctggc
      241 gctgcgtcaa cgcgctggcg gcgatcaggt ccggccaatg ccggacagcc agcttgaccg
      301 cgccggcgat ggcggttgcc gccggctttt ccgggatgtc caatttggcg caaaaggtgc
      361 gcagcagcgc cggcgtgact tgcggcctgc cggccctggc gggctcgccg gccttcagcc
      421 gcgccttggg cgcctgcggg tcgggcggca acagatgcaa ggcatggcct tgccgcatgc
      481 cgtaggccgc gtaggccacg atgtcatagg ccggggacag ttcgggcgtg cggccgtccc
      541 gatatatcac gccgatgttc ttcaagtgca tgtccacgtt gcccagcagc tcgttgacca
      601 ccagccgccg cagcagctcg tgcacggcgg gctcgcccat ccggttgggc tggcctgcat
      661 aagccttggg cggggccatc aagagcgcgg cgatctccag gtagctgcgc gaatacttgc
      721 gcgcgggcac catgcccagg atctgcccga agtcctccac gtgggtgcgg ctgccgtcgg
      781 catgccggtc gaaccgctcc acggccagga accgggtttg cgcatggaca tcgtcgccca
      841 ggtcgtagcc gtgctcggcc tcgagctttt ccaggggttc gagtttcgct tcgcaggtgt
      901 cgaccccggc cgcctgggca aggagcagcg acaggtgctc gacctcgggc aggaggggat
      961 agcccacggt cggcagcttg gcgatgatcc gcgtgtcgcc cagccgggtg cgggcgacgt
     1021 agcgtccgtc cttttccgtc acgccgatct tgggctgcac gcccgatatc gacatggccg
     1081 tgtccagggg cgcctgcatg acgctcatct ccagcgcgtc ggcgtcctgg gtgacgaggt
     1141 gggtcatgtc gcgaggggtc agctcgatgg gccgggccgt caggttgccg ggcagatcga
     1201 ggccgcccgc ggccagcagt tccaggtggt catgcgggtc gcagccgcgc aactgggcca
     1261 gatgctcgcg gaataccccc tcgggcagca ggccttggaa aaacgcgggc agccaggtgt
     1321 tgccgtggcc ggcgggctgg ccgttgaaga tcggcgcggt tctgttcagc cacaaggcac
     1381 gctgcgcgtc cggggtcggc gcgagcatcg acaggctcaa gaccggcgcg tcgggccgtg
     1441 ccgcgaaagc ctcgctggcc tcgaagcgga tcacggggtc ttcgccttgt tcgccatact
     1501 ggaacagaat gccggccggc tgtcccgcca gcaggatctc cagggcgcgc acgttcattt
     1561 gagccgctcc agcgccgcgt cgacgccggt ttgcatggcg ggggcttgct ccgcttgcag
     1621 cgcctgcgcc gcctttttgg gcaccagcac gacttccagc ccaaggcgat cggccaccga
     1681 cagcagggtg cttaccttga agtcgtgctc gcccgacagc acgcgccgca gccccagcgc
     1741 ggacaggccg gtcagtttct ggagcgcttg atgggcgatc ttggccttgc ggcgttcggc
     1801 ggcaagcgtg gccgccagtt ccgggatgga tgtcatggta actacagtat ctttttcgat
     1861 agaaaagata ctgtagttgc tttcttgaga tgaatcaata aaaagacact gcagttactc
     1921 tttttctaaa aaagacactg cagtgtcgtt tttggaccgg ctttacgcca gctcttcgtc
     1981 cttcaaggcc gccgccacgc cgggattggc ctgcatgcgt tccacgaact tgggcagctg
     2041 ggccaggccg gacaggtcga tgtgcgtggc gacggcccag cgcagcacga cgaacagata
     2101 ggcgtcggcg gcggtgggct tggcgccggt gagatagtcg cggccggtga gctgggtgtc
     2161 gatgatggtg aagtagtcgc gcagcgtctg ggcggcctgg ctgcgcagct cctcctgggc
     2221 gacttcgctg ctcacgaagc gctgggcgcc gaagatccgg ctgaaggtgc ggtgtatgtc
     2281 ggaattgatg aaggccagcc agcgcagggt ttcggcctgg ctcttgggcg tgccgtcgcc
     2341 cagcaggccc gcttcgggat atttggcggc cagataggag acgatggcca cgttctgcgt
     2401 cagggtgaag tcgccgtcgc tcaaggcggg cacggagccc acggggttga tcttgaagta
     2461 ctcgggcgtc ttcagggcgc tgcgttccat tttctgggcc tcgaagggct tgccgatcca
     2521 ctccagggcg atgtgtatgg tcagcgagca ggcgccgggc aagtaataca gcttcatgag
     2581 tctctcctgt ggcatgagct tatgctgccc agtatggtac gtcaggcggg ggctttccag
     2641 acagcccagg ccgtcatggc ccaggccagc aggaaggcca cgccgcccag cggggtgatg
     2701 gcgcccagcc agcgggcgcc ggtcagggtg agcacgtaga ggctgcccga aaacagcacg
     2761 atgccggcga acatggccac gcccgcagcg ggcagcaggc cggccgcggg gtagcgcgag
     2821 caaagccagg ccacgacgaa caggcccagc gcatggatca ggtggtactg cacgccggtc
     2881 tgccagatgc ctagcagttc ggcgtcgacc atgcgcttga ggccgtgggc cccgaaggcg
     2941 ccggcggcga cggcgaagat catgttgagg gcggcgagca cggtgagctg gcgttcggtc
     3001 ataacgggag taggtcgcgg gaaaacccga ttatgatgcg gaattcgccg ggccgggggc
     3061 ctgtgtccac tatgatgttt gccgggagcg tattcatgcg gtatgggatg tttctgcggc
     3121 ttgggcgccg tgccctggcg gcggcgctga tgatggcggc ggccggcttg gcccaggcgg
     3181 ccgagccttt gcgcatcggc gtgatcgcca gcgtggccaa cgaagcaacc gagatcgcga
     3241 tcgcccaggc caagaagcag ggcctggatg tgaagctggt ggaattcagc gactgggtga
     3301 tgcccaatgt ggcggtggcc gacgggtcga tcgacgccaa cttcttccag cacgagcctt
     3361 tcctggatct gttcaacaag agccgcgaca cccacctcat gccgatcgcc tacggctatt
     3421 ccacgaccat cggggtgttc tccaagaagc tcaagcccgg cgacgcgatc ccgcggggcg
     3481 ccaccctcgg cgtgcccagc gacccggtga acacgggccg cgcgctgctg ctcctgcagt
     3541 ccatgggcgt gctcaagctg cgcgccggca cgggcaatat ggcggccacc accgacgtgg
     3601 tcgataatcc caggcaggtc aagctggtct cgatcgaggg cgcgcagtcc gcgcgggtgt
     3661 tcgacgatgt gacggcctcg gtgacgtaca cgaccttcgc caagcaggcc ggcatcgacg
     3721 agaaggacgg gctggccttc gacaataccg acccggaggt ggtcaagcac tacgccatcc
     3781 gctgggtggc gcgcccggac aaggccaatg atccgcgcct gctgcagttc atcaagattt
     3841 accagtcttc accggaggta aaggccacct tgcgccgcct ctacggaagc ctgatcgatt
     3901 ttccctggta ggcggaaact ctcacggagg ccggatggag catagcgttc ccctgatcac
     3961 cacgctggcg gcaggcttcg ggatggcgtt gatcctgggc ttcgtggccg agcggctcaa
     4021 ggtgcctgcg ctggtcggct acctgatagc cggcatcatc atcggcccgg ccacgccggg
     4081 cttcgtggcc gacgtgcaac tggcctcgga gctgtctgaa atcggcgtga tgctgctgat
     4141 gttcggcgtg gggctgcatt tctcgctcca ggacctgctc gacgtcaagc acctggccgt
     4201 gccgggcgcg gtggtgcaga tggccctggc caccgcgctg ggcatgctgc tggcctggtg
     4261 gtggggctgg agctggggcg gggggctgat cttcggcctg tccctgtcgt gcgccagcac
     4321 cgtggtgttg ctcaaggggc tggaggcgcg cggcgtgctt gagaccctga acggccgcat
     4381 cgccgtgggc tggctggtgg tggaagacct cgccaccgtg ctggtgctgg tgctgctgcc
     4441 gccgctggcc ggcgtgctgg gcggccatgt gccgcagcac ggcgtggtcg acgcgccttt
     4501 gtggctcacc atcggcaaga ccttgctgca ggtggccgtc ttcatcggcc tgatgctggt
     4561 cgtcggccgc cgcgcgctgc cgtggctgct gtggcaggtg tcgcgcaccg gttcgcgcga
     4621 gctgttcacc ttgtgcgtca tcgccgtggc catcggcatt gcctacggcg cggcgcagct
     4681 gttcagcgtg tcgttcgccc tgggggcctt ctttgccggc atggtgatgc gcgagtcgga
     4741 gttcagccac cgggcggccg aggagtccct gccgctgcgc gatgccttct cggtgctgtt
     4801 cttcgtgtcg gtgggcatgc tggtcgaccc cgccatcctg gtccggcagc cgctgcacgt
     4861 gctcggcgtg ctgctcatca tcatgttcgg caagtcgctg gcggcgatgg cgctggtgct
     4921 ggtcttccgc tatccgctca atacggccct gatcgtgtcg gccagcctgg cgcagatcgg
     4981 cgagttttcg ttcatcctgg ccgggctggg ggtgtcgctg ggcctgctgc cttccgaggg
     5041 catgagcctg gtgctggccg gggcgctgat ttccattgcg ctcaacccgg tgctgttcgc
     5101 cgcgatcgag cccctgcgcc gctggatcct ggcccggtcg gcgctggccc ggcggctcga
     5161 ggcgcgcggc gacccctatg ccgaactgcc cccgagcacc gagcgcaaat acctggaagg
     5221 ccaggtggtg ctggtggggt acggccgggt ggggcggcgc attgccgccg aactggtggc
     5281 gcacgccatt cccttcgtgg tggccgaaca gaaccgcgaa cgggtcgagg agctgcgcaa
     5341 gcagggcatg gcggccgtct cgggcgacgc ggccgagccc gcggtgctga tccaggccca
     5401 tatcgcccac gccgccatgc tggtggtggc cgcgccggat ccggtggacg tgcgcaagat
     5461 ggtggatacg gcgcgcaagc tcaacccggg catcgagatc gtcctgcgca cgcacagcga
     5521 ggacgaatcg catctgctgc gcaaggagaa cctcggcgcg gtgttcttcg gcgaagagga
     5581 gcttgcccgc ggcatggcgg ggcacgtgct ggcccgcttc gcgccgcact aggcgctata
     5641 gggcgtggcc caggcgctgg cccatgcgca cggcattgcc gggcgcggcg agctccgccc
     5701 aatccacgcg gccgggaccg aacagggcaa cgacggtcga gcccagcagg aagcggccca
     5761 tttcggcgcc ttgcgccagc cgcgcggcct gtccgccctc gtagcgggtg gcgacgaccc
     5821 cggaccggtg cggcgcgatg cggccggccc agacggtctc gacggaggca acgatcatgg
     5881 cgcccaccag caccagggcc atggggccgt gtccgggcgt gtcgaaaatg cacgccacgc
     5941 gttcgttgcg ggcgaagagt tcgggcacgt tgcgggcggt gagcgggttg accgaataca
     6001 gcttgccggg cacgtagatc atctcgcgca gggtgccggg caggggcatg tgcacgcggt
     6061 ggtagtcgcg cggcgacagg tagatggtgg cgaagctgcc gccctcgaaa ggggcggcgc
     6121 gcgccgcgtc gccgcccagc agggcggcaa gtccgaagtc atggcccttg gcctggaaga
     6181 tgcggccgtc gacgatgcgg ccggcctggc tgacgctgcc gtcggccggg cacagcacgg
     6241 cgccgggcgt ggcgtccagc ggccgggcgc cgggcttgag cgcgcgggtg aagaaggcgt
     6301 tgaagctttc gtaggccaat gggtcctcga cccgcgcttc ctgcatggcc acgccgtagc
     6361 gccgggcaaa gcggccgatg aggacgttct tgagccaggg cgcgcggctg tcggccaggc
     6421 tgcccgccag ccgcgaaatc aggtggtgcg gggcaaggcg ctgacaggcg agaaaaagcg
     6481 agtcgttgaa agacatgtcg gcagacgaaa atcgcgcctg ccggatgcgg gcgccggggg
     6541 gcaagtgtag cgtgaagcgc cggcccggcc tgtcaggagg cgtcggcggg cggggtttcg
     6601 gccggggggg cgactggcgt ttcggccggc gtttcggcgg gctgggcctg ctgcgcttcg
     6661 atgtcggcca gggccgctgc ggccgcggcc ttgacggcca tggcgtcctc cagcgtttcg
     6721 accggggtgc cgggcgggat ggatgtgcgg cggtcgcggt tgatgctgcg gggcgccgac
     6781 acgttgtggc ggcgcgatgc ggcgtaatgc agcagcaccg acagcaccga gcccttgtcc
     6841 tggatccatt gggtgcggtt gcgcaggtgc ttcatggcct gttcgggcgt acccagccgg
     6901 tagttcgagt cattgatgac ggcggtggat ttgccgtcga atttctcgtc gggcaggtcg
     6961 gtgatgccga cggcgtcgag gttgtggctg tcgaaatggg cggtgtagac gagcttgagc
     7021 ccgctgtgcg ggtcttgtac gacgaccagc ttttcaatgg acgagatgtc gttgatgctg
     7081 ccgatgaggc tcgactcgac cagctggttg ccacgttgct cttccgtata gagaaaaatg
     7141 gtgggcttgg actttgcgtc gatgctcatg ggtgttccgg gcgaaagtcg gccggacagc
     7201 gcgaggcaat gtacggccgc tacataaagt aggcagtcgt aacattttat cgcaacacat
     7261 tattacaaca gcctgaaatc cgactgtttt gtcctggctt gtcccggatt gcccgacgct
     7321 cagtggggtg tgttgcccga ccccatgtcc caatacaccc cggccatcag gcacagggct
     7381 tcgcgcagca gttcgggcgg caggtgctcg ttgggcgcgt gctgcgagca gccggggtag
     7441 gagtgcggaa tccacaccgt gcacaagccc agcacgtcgg tgaagatgtc gttgggcagg
     7501 gagccgccca ggttggggag gatggcgggc ttcttgccgg tggtttgctc cagcgactgc
     7561 acggcccact gcacccaggg gttgtccggg tcgatgcggg tggctttgaa tagggtctcg
     7621 cgcgtggtgg cgatcttcac catgggaaag ccgtggcggt ccaggtggcg gcgcagggcc
     7681 ggcagcaggt cctcggtgtc gaggcccacc acgaagcgca gctggcagcg cgcccaggcg
     7741 cgcggcggga tggcgttgac gggcgtgtcg gggttgccgg tcttgtaggc cagcacctcg
     7801 aaggagttcc agccgtagac cttctcggcg ggcgtgaggc cgggctcgcc ccagccgggt
     7861 tcgatgacgg ggccgtcggc gccgccgtcg acctcgcaat cggccagcac gcggcgcacc
     7921 gaggcgggca gctccttggg gcgccattcg ggcaccagca cctggccggt gggcgagacc
     7981 agggtggcga tggcgtgggc cagctggat
//
LOCUS       DASWPO010000340        13419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_3116015, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000340 DASWPO010000000
VERSION     DASWPO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 13419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13419
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3116015"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGC69_18025"
     CDS             complement(<1..271)
                     /locus_tag="VGC69_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEY0297230.1"
                     /translation="MSDVTIYHNPNCGTSRNVLEIIRGHGIEPTVVQYLKTPPSRATV
                     KKMIADAGLTVRQALRVKGSPYEELGLADPKWTDAQLLDFIEQHPI"
     gene            complement(275..1270)
                     /locus_tag="VGC69_18030"
     CDS             complement(275..1270)
                     /locus_tag="VGC69_18030"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0297231.1"
                     /translation="MKICIYGLGAIGGLLGARLAAAGEDVSAVTRRGATLTAVQQRGL
                     TLIETEESGSHVRHIPIHAVEDPAELGPQDLVIVAVKTTSLADAAQRIGPLLGPGTTV
                     LSAMNGVPWWFFHGLGPEFAGTPLPSVDRDGRLAAAIPMARIVGCVVHLSSATPAPGV
                     VQHVIGNRLIIGEPTGGADTPRAQAVIAALRRAGFDVEASRQIQHDIWFKLLGNMTVN
                     PVSAMTGATGDRILADDLVRGFMSRCMLEAAAVGERIGIPVVGTPEDRHAVTRKMGAF
                     RTSMLQDVEAGRKVELDALVGTVRDIAQSVKVDTPNIDALFGLARLHALVQGLYD"
     gene            complement(1284..2024)
                     /locus_tag="VGC69_18035"
     CDS             complement(1284..2024)
                     /locus_tag="VGC69_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0297232.1"
                     /translation="MEQTTPEERALRLELAACYRIFAMQGWTELIYNHITLRIPGPER
                     HFLINPFGLHYSEVTASNLVKIDLQGRVIGESQWPVNPAGFTLHSAIHQGIADAHCVM
                     HTHTTAGCAVANSKAGLDMSNFYSAMLYDRVAYHDFEGITVHPEEGPRVARSIGNRQA
                     VILRNHGLLTWGVSLPQAFTYMWTLNRACEIQVAGAALGPSMPIPLDIQKQCSADALQ
                     FDPTFGAGRDVFDALVRQIDRIDDAYRH"
     gene            complement(2079..2468)
                     /gene="trxA"
                     /locus_tag="VGC69_18040"
     CDS             complement(2079..2468)
                     /gene="trxA"
                     /locus_tag="VGC69_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0297233.1"
                     /translation="MSIVELTQDTFQQAVEKPDGTLIVDFWAPWCGPCRGFAPVFEKA
                     AEAHPDITFGKINTDVEQELAGTLGIRSIPTLMVFREQVLLFSQPGALSAGQLNELLA
                     QVKDVDMAQVHQEIAEARAKGDGQAQA"
     gene            complement(2515..3546)
                     /locus_tag="VGC69_18045"
     CDS             complement(2515..3546)
                     /locus_tag="VGC69_18045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0297234.1"
                     /translation="MSSLANVPFSVLDLAPVVQGGTAADAFRNTARLAQHVEKLGYQR
                     FWLAEHHNIPGVASSATAVLIGHVAAHTTTLRVGSGGVMLPNHAPLLIAEQFGTLESL
                     FPGRIDLGLGRAPGSDGITQHALRRSPRSGLEFPELLAELRAFLAEPRIGQTVHAYPG
                     EGLSNLPIWLLGSSDFSARLAAELGLPFSFAGHFSPEGMAAMRLYRHLFKPSEALPKP
                     YAMIGVPVIAADTDEHARYLSTSQQQKFLSMVRNNRQPMQPPVETMDGIWTPWERDAV
                     QQRLAASIVGGPDTVRRGLEALIEETLADEVMIVSDFYHAEDRLRSYEIVAAIKQAGQ
                     AREPAQAGI"
     gene            complement(3706..5043)
                     /locus_tag="VGC69_18050"
     CDS             complement(3706..5043)
                     /locus_tag="VGC69_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEY0297235.1"
                     /translation="MLEKLFQLRANGTTVRIEIVAGLTTFLTMSYIIFVNPSILSATG
                     MDRDAVFVATCLAAAIGTLVMALVANWPIGMAPGMGLNAFFAFAVVKGMGYSWEQALG
                     AVFISGVIFVALTLTGIRAWLIKGIPNSLRSAIAAGIGLFLAIIALSSADIIVASPAT
                     KVTLGDLSAPGPLFAILGFFIIAVLDALRVRGAILIGVLVITVLSMLLGYNAFHGVFA
                     APPSLSPTFFKLDIAGALNSGLFHVILVFVLVEVFDATGTLMGIAKRAGLIDTSQAAM
                     QQPATPAQSQAKANRLGRALFSDSIAILAGSLLGTSSTTAYIESASGVQAGGRTGLTA
                     LVVGVLFLAALFISPLAGSVPAYATAPALLYVAGLMMRELVDIDWSDVTEATPAALTA
                     LIMPFTYSIANGLAFGFISYAVLKTCTGRIREVHPATWLVAILFVIRFAALPG"
     gene            5105..6139
                     /gene="tsaD"
                     /locus_tag="VGC69_18055"
     CDS             5105..6139
                     /gene="tsaD"
                     /locus_tag="VGC69_18055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0297236.1"
                     /translation="MIILGFESSCDETGVAAVCTERGLLAHALHSQIAMHQEYGGVVP
                     ELASRDHIRRVVPLTRQVLDEAGLALADVDVVAYTAGPGLAGALLVGASVAQSLAWSL
                     GRPALGIHHLEGHLLSPLLADPRPEFPFVALLVSGGHTQLMRVDGVGRYELLGETLDD
                     AAGEAFDKSAKLLGLSYPGGPALSRLAEKGDATRFDLPRPMLHSGDLDFSFSGLKTAV
                     LTRVRAAEQAGGLDEQARADLAAVTQTAIVDVLAGKAIKALRQTGLKRLVVAGGVGAN
                     SLLRAKLAQALRPMKAQAYFPPLALCTDNGAMIAFAAAERVKHGLVSLEEGNHAFTVK
                     PRWDLAEISE"
     gene            complement(6298..6936)
                     /gene="plsY"
                     /locus_tag="VGC69_18060"
     CDS             complement(6298..6936)
                     /gene="plsY"
                     /locus_tag="VGC69_18060"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY0297237.1"
                     /translation="MAFDVSSPYFYAMLVLLAYLIGSIPFAVVVSKLLGLQDPRSYGS
                     KNPGATNVLRSGSKVAAVLTLLGDAAKGWFALWLVQHLVPDLPWPVYALAALAVFLGH
                     LYPVFLGFKGGKGVATALGVLLAVQPWLALAVALTWLLVAVVTRYSSLAALVAAVCAP
                     LYYLLGGKLGWAVHPPVSASLVIIALLLIYRHKTNIARLVGGAESKIGAKKK"
     gene            7105..7851
                     /gene="surE"
                     /locus_tag="VGC69_18065"
     CDS             7105..7851
                     /gene="surE"
                     /locus_tag="VGC69_18065"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0297238.1"
                     /translation="MRILVSNDDGYNAPGIEALVRALSGLGEITVVAPETNHSGASNS
                     LTLNRPLSVRTASNGFYYVNGTPSDCVHVALTGGLMGSRPDLVVSGINNGANMGDDTL
                     YSGTVAAASEGYLFGIPAIAFSLAEKGWAHIDDAARVARQVTERQLANPLGGPVLLNV
                     NIPNRRFEDMAGYAVTRLGKRHPSEPVVRSTTPYGDTVYWIGPAGSAADATPGTDFHA
                     LAQGCVALTPLRLDLTQYEQLDAVRGWVEK"
     gene            8054..8755
                     /locus_tag="VGC69_18070"
     CDS             8054..8755
                     /locus_tag="VGC69_18070"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0297239.1"
                     /translation="MPRPNQAPAMSSAAPAGGNLGLNSDRLRHAMVQRLRAQGIVDER
                     VLGAMSAVPRHIFVDEALASRAYEDAALPIGHGQTISQPWVVAHMLATVCENRQPTRV
                     LEVGAGCGYQSAVLAQFVREIYAIERIRGLYELAREQLRALRLASRVRLIHGDGMLGQ
                     PGAAPFDAIVVAAAGLAIPQALLEQLALGGRLIAPEGGAEQRLVLMERTGPASWTRRE
                     LEAVRFVPLRAGIQS"
     gene            8773..9663
                     /locus_tag="VGC69_18075"
     CDS             8773..9663
                     /locus_tag="VGC69_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0297240.1"
                     /translation="MLNGQFALNDAIPPGASARLSRQVLPLVALTLLALAGCSSEPSR
                     QAPIVDLNEPSSGLSAGGTYVVKPGDSLYGIARRNGVDIESLKRANNIADPNHLSVGQ
                     VLKIPGAGGSGPVPVAPATPQPTPVPPAASKPAEPVPLDQLPPASGQPPEPPAGTAAV
                     PAVTAPPAAARASDANVINWGWPAQGQISQPFNASSKGIDIAGNLGDPITSAADGKVM
                     YSGNGVRGLGNLVIINHSNGFITAYAHNQDLLVKTGQDVKRGAKIATLGQSDAASPLL
                     HFEIRRQGTPVDPLQYLPAR"
     gene            9692..10471
                     /locus_tag="VGC69_18080"
     CDS             9692..10471
                     /locus_tag="VGC69_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0297241.1"
                     /translation="MTPTLVFDLETLPDVVGLRRLNGWGPEVSDLEVAERAFAARREA
                     TGGDFLPLHLQRVAVVGCVFRDDQGFRVKTLGNPDDPEPVLLAGFFKTIERYTPRLVS
                     WNGSGFDLPVLHYRSLIHGVPAPRYWDMGEDDRDFKYNNYISRYHNRHTDLMDVLAKY
                     NGRANAPLDDLAKLCGFPGKLGMDGSKVWEAWNNGQADAVRAYCETDVVNTWLVYCRF
                     RFLRGELDAAAYQAEIDLVRDTLAASGAPHWKEYLAAWDAA"
     gene            10592..11212
                     /locus_tag="VGC69_18085"
     CDS             10592..11212
                     /locus_tag="VGC69_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297242.1"
                     /translation="MSRTTLRASVAGLAMALASGAAIPVFAATAAAASPAAGEAAQPP
                     QHPGHWQHGAKGRKPHGGMPMMMWREGLMIPGLGPISKPQVEALKLDANQQALLKQAR
                     DAQGDLFKARREAMVKQRGLIDQQTTAGKLDPRALASAADANRDQFRGQATQLRDKWL
                     AVWDSLNDTQRTQVAGFVKEREARMNDMHERMEHKGHSKQQGPAAG"
     gene            11653..12411
                     /locus_tag="VGC69_18090"
     CDS             11653..12411
                     /locus_tag="VGC69_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY0297243.1"
                     /translation="MKKKLLALPLAFGACFGSAAHAADGTDLGGGFSLTGNAALVSDY
                     RFRGYSQTNMLPAVQGGIDLTHSSGFYLGNWDSSISDKVFTDGVVEMDFYGGWKGDIG
                     HGLGLDVGGLEYVYPGSTSPKGNIYNNTDLYMGLSYDAYTLKFSYTPTDFFSVPNSKN
                     TWYIDGSANWDLGSGWGLNAHVGYQKLMGNARFGADGNTYSGIVDYKLGVTKDLSGWV
                     LGLAMVGTDHQRYIVTPHGAAGRFGVVASVAHTF"
     gene            12505..>13419
                     /gene="rlmD"
                     /locus_tag="VGC69_18095"
     CDS             12505..>13419
                     /gene="rlmD"
                     /locus_tag="VGC69_18095"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEY0297244.1"
                     /translation="MPEVLNIESLDLEARGIARRDGKVIFVEGALPGERVTVSTVRRK
                     PSYEIARVDEVLSPSSQRVEPRCPHFSVCGGCAMQHLEPSAQVAIKQRALEDTFVHIG
                     KLKPARMLPPLHGPTWGYRYRARLSVRVVPKKGGVLVGFREKKSSYVVDMRECHVLPR
                     HVSDLLLPLRAMIAAMSAPARLPQIEVALGDAATVLVLRHLEPLTGGDIAVLRAFAAE
                     HGVQWWLQPKGPDTVHALEPEHADALAYALPEFGLRMPYRPTDFTQVNHAINRALISR
                     ALTLLGVQPTDRVADLFCGLGNFTLPLAT"
BASE COUNT         2290 a         4406 c         4574 g         2149 t
ORIGIN      
        1 ggatggggtg ctgttcgatg aagtccagca actgcgcatc cgtccacttg ggatcggcca
       61 ggcccagttc ttcgtaaggc gagcccttga cgcgcaaggc ctggcgcacg gtcaggccgg
      121 cgtcggcgat catcttcttc acggtcgcgc ggctgggcgg ggttttcagg tactggacga
      181 ccgtgggctc gatgccgtgc ccgcggatga tctccagcac attgcgcgaa gtgccgcagt
      241 tggggttgtg atagatggtg acgtcgctca tgcgtcaatc atacaaccct tgcaccaggg
      301 catgcagccg ggccagcccg aagagggcgt cgatattggg cgtgtcgact ttcacggatt
      361 gggcgatatc gcgcacggtg ccgaccaggg cgtccagctc gaccttgcgg ccggcctcca
      421 cgtcctgcag catggacgtg cggaaggctc ccatcttgcg cgtgacggca tggcggtctt
      481 ccggcgtgcc aacgacgggg atgccgatgc gctcgccgac ggccgcggct tcgagcatgc
      541 agcgcgacat gaagccgcgc accaggtcgt ccgccaggat gcggtcgccc gtggcgccgg
      601 tcatggccga aaccgggttg accgtcatat tgcccaggag cttgaaccag atgtcgtgct
      661 ggatctggcg ggacgcctcg acgtcgaacc cggcccggcg cagggcggcg atgacggctt
      721 gcgcccgcgg cgtgtcggcg ccgcccgtgg gctcgccgat gatgaggcgg ttgccgatca
      781 catgctgcac cacgccgggc gcgggggtgg ccgaggacag gtgcaccacg cagcccacga
      841 tgcgggccat tgggatggcg gcggcgaggc ggccgtcgcg gtcgaccgag ggcaggggcg
      901 tcccggcgaa ctcggggccc aggccgtgga agaaccacca gggaacgccg ttcatggccg
      961 agagcacggt ggtgccgggt cccagcagcg ggccgatgcg ctgggccgcg tcggccaggg
     1021 aggtggtctt gacggcgacg atcaccaggt cctggggccc gagctcggca gggtcttcga
     1081 cggcatggat ggggatgtgg cgcacatgcg aaccgctttc ctcggtctcg atgagcgtca
     1141 ggccgcgctg ctgcacggcg gtcaatgtgg cgccgcgccg cgtcacggcg ctgacgtcct
     1201 ctcccgcggc ggccagccgc gcgcccagca gcccgccgat ggcgcccagg ccgtagatgc
     1261 agattttcat gaggcgagct cccttagtgg cggtaggcgt cgtcgatgcg gtcgatctgg
     1321 cggaccagcg catcgaacac atcgcggccg gcgccgaatg tcgggtcgaa ctgcagcgcg
     1381 tcggcgctgc actgtttctg gatatcgagc gggatgggca tgctcggccc gagcgccgcg
     1441 ccggcgacct ggatctcgca ggcgcggttc agggtccaca tataggtgaa ggcctgcggc
     1501 agggaaacgc cccaggtgag caggccgtgg ttgcgcagga tcacggcctg gcggttgccg
     1561 atggagcgcg ccacgcgcgg gccttcttcg gggtgcacgg tgatgccctc gaagtcatgg
     1621 taggccacgc ggtcgtacag catggcggag tagaagttcg acatgtccag cccggccttg
     1681 ctgttggcga ccgcgcaacc cgccgtggtg tgggtgtgca tgacgcagtg ggcgtcggcg
     1741 atgccctggt ggatggccga atggagcgtg aagccggcgg ggttgaccgg ccattgcgat
     1801 tcgccgatga cgcggccctg caggtcgatc ttgaccaggt tggaggcggt cacctcgctg
     1861 taatgcagtc cgaaggggtt gatcaggaaa tgccgttccg gacccgggat gcgcagcgtg
     1921 atgtggttgt agatgagctc ggtccagccc tgcatcgcga agatgcggta gcaggcggcg
     1981 agttccaggc gcagggcgcg ctcttcggga gtggtctgtt ccatggcggc gacgggtcta
     2041 taggcgctgc ttgcatcgtg gcaggcctcg cggggcggtc aggcctgcgc ctggccatcg
     2101 cccttggcgc gggcttcggc gatttcctgg tgcacctggg ccatgtcgac gtccttgacc
     2161 tgggccagca gctcgttgag ctggccggcc gacagggcgc cgggctgcga gaacagcagc
     2221 acctgctcgc gaaagaccat cagcgtggga atcgagcgga tgcccagggt gccggccagt
     2281 tcctgctcga catcggtgtt gatcttgccg aaggtgatgt ccggatgcgc ttcggcggct
     2341 ttctcgaaca cgggcgcaaa gccgcggcag gggccgcacc agggcgccca gaagtcgacg
     2401 atgagcgtgc cgtcgggttt ttccacggct tgctggaaag tgtcttgggt gagttcaaca
     2461 atgctcatgg ccttgtcctt gccgcgtcca tgcgcggaga tgaaaaaaag cggttcagat
     2521 gcctgcctgg gcaggttctc gtgcttgccc ggcctgcttg atcgcagcga cgatttcgta
     2581 ggaacgcagc cggtcttccg catggtagaa gtcggagacg atcatgacct cgtcggccag
     2641 ggtttcttcg atcagcgctt ccaggccgcg acgcacggtg tccggcccgc cgacgatgga
     2701 ggcggccagc cgctgctgca cggcgtcgcg ctcccagggc gtccatatcc cgtccatggt
     2761 ttccacgggc ggctgcatgg gctggcggtt gttgcgcacc atgctcagga atttctgctg
     2821 ctgcgaggtc gacaggtagc gggcgtgctc gtcggtgtcg gcggcgatca cgggcacgcc
     2881 gatcatggca tagggcttag gcagcgcctc ggagggcttg aacaggtgcc ggtacaggcg
     2941 catcgccgcc atgccttcgg gcgagaagtg gcccgcgaac gaaaagggca ggcccagctc
     3001 ggcggccagg cgggcgctga aatcgctcga gcccagcagc cagatgggaa ggttcgacag
     3061 cccttcgccc gggtaggcgt gcaccgtctg tccgatgcgc ggttcggcca ggaaggcgcg
     3121 cagctcggcc agcagttcgg ggaattccag gccgctgcgc ggcgagcggc gcagcgcgtg
     3181 ctgggtgatg ccgtcgctgc cgggcgcgcg gcccaggccc aggtcgatgc ggccgggaaa
     3241 gagcgactcc agcgtgccga actgttccgc gatcagcagc ggagcatggt tgggcagcat
     3301 cacgccgccc gagcccacgc gcagcgtggt ggtgtgcgcg gccacgtggc cgatcagcac
     3361 ggccgtggcg ctgctggcca cgccgggaat gttgtggtgt tcggccagcc agaaacgctg
     3421 atagccgagc ttttcgacat gctgggccag ccgggccgta ttgcggaagg cgtcggcggc
     3481 ggtgccccct tgaacgacgg gggcgagatc cagcaccgaa aagggcacat tggccaggct
     3541 gctcatggat tcctcacgtt ttattcatta tctatgtggg gagtgtagcc atgatttcaa
     3601 gcctggaccg gtgcaaccct gcgggcagcc ggggcgatag ccccgctgta tgggagcgat
     3661 tgacgcggcc tggcgcccgg cgcggggccg ggcgccagct gggcgtcagc cgggtagggc
     3721 ggcgaagcgg atgacgaaca ggatggccac cagccaggtc gccgggtgca cctcgcggat
     3781 gcggccggtg caggtcttga gcacggcgta gctgatgaag ccgaaggcca ggccgttggc
     3841 gatggaatag gtaaagggca tgatcagcgc ggtgagcgcg gccggggtgg cctcggtcac
     3901 gtcgctccaa tcgatgtcga ccagctcgcg catcatcagg ccggccacgt agagcagggc
     3961 gggcgcggtg gcgtaggccg gcaccgagcc ggccagcggc gaaatgaaga gcgcggccag
     4021 gaacagcacg cccaccacca gggcggtcag gccggtgcgc ccgcccgctt gcacgcccga
     4081 ggcgctttcg atgtaggcgg tggtgctgct ggtgcccagc agcgaaccgg ccaggatggc
     4141 gatgctgtcg gagaacagcg cgcggcccag gcggttggcc ttggcctggg actgggccgg
     4201 cgtggcgggt tgctgcatgg cggcctggct ggtgtcgatc aggccggcgc gcttggcgat
     4261 gcccatcagc gtgccggtgg cgtcgaagac ctcgaccagc acgaacacca ggatcacatg
     4321 gaacaggccc gagttcagcg cgccggcgat atccagcttg aagaacgtgg gcgacaggct
     4381 gggcggcgcg gcgaacacgc cgtggaacgc gttgtagccc agcagcatgg acaggaccgt
     4441 aatcaccagc acgccgatca ggatggcgcc gcgcacgcgc agcgcatcca gcacggcgat
     4501 gatgaagaag cccaggatgg cgaacagcgg gcccggcgcg ctcaggtcgc ccagcgtgac
     4561 cttggtggcc ggactggcca cgatgatgtc ggcgctggag agggcgatga tggccaggaa
     4621 cagcccgatg ccggccgcga tggcgctgcg cagcgagttg gggatgccct tgatcagcca
     4681 ggcgcggatg ccggtgagcg tgagcgcgac gaagatcacg cccgagatga acacggcgcc
     4741 cagggcctgc tcccagctgt agcccatgcc cttgaccacg gcgaaggcga agaaggcgtt
     4801 caggcccatg ccgggcgcca tgccgatggg ccagttggcg accagcgcca tgaccagcgt
     4861 gccgatggcc gcggccaggc aggtggccac gaacaccgcg tcgcggtcca tgccggtggc
     4921 cgacaggatc gagggattga cgaagatgat gtaggacatc gtcaggaaag tcgtcaggcc
     4981 cgccacgatc tcgatgcgaa ccgtggtgcc gttcgcgcgc aattgaaaga gtttttccag
     5041 catggtttcg gttcttcttg ggttggaaag cgcgtatttt ctcaccggtt taaaatccag
     5101 gtcgatgatt attctgggct tcgagagttc ttgcgacgaa accggcgtcg cggcggtctg
     5161 caccgagcgc ggcctgcttg cgcacgccct gcacagccag atcgccatgc accaggaata
     5221 cggcggcgtg gtgcccgagc tggcctcgcg cgaccatatc cgccgcgtgg tgccgctgac
     5281 ccgccaggtg ctggacgagg ccgggctggc gctggccgac gtcgacgtcg ttgcctatac
     5341 cgccggcccg gggctggcgg gggcgctgct ggtgggcgcc agcgtggccc agtcgctggc
     5401 ctggtcgctg ggtcggccgg ccctcggcat ccatcatctc gaaggccacc tgctgtcgcc
     5461 gctcctggcc gacccgcgcc ccgagtttcc cttcgtcgcc ctgctggttt ccggcggcca
     5521 cacccagctc atgcgggtgg acggcgtggg gcgatacgaa ctgttgggcg agaccctgga
     5581 cgacgccgcc ggcgaggcct tcgacaagtc ggccaagctg ctgggcctga gctaccccgg
     5641 cggcccggca ctgtcgcgcc tggccgaaaa aggcgatgcc acccgcttcg acctgccccg
     5701 gcccatgctg cacagcggcg acctggactt cagcttcagc ggcctgaaaa ccgcggtgct
     5761 gacgcgcgtg cgggcggccg agcaggccgg cggcctcgac gaacaggccc gcgccgacct
     5821 ggccgccgtc acgcagacag ccatcgtcga cgtgctggcc ggcaaggcca tcaaggcgct
     5881 caggcagacc ggcctgaagc ggctggtggt ggccggcggc gtcggcgcga attcgctgct
     5941 gcgcgccaaa ttggcccagg cgctcaggcc catgaaggcc caggcctatt tccccccgct
     6001 ggccctgtgc accgacaacg gcgccatgat cgctttcgcg gcggcggagc gggtcaagca
     6061 cggcctggta tcgctggaag aaggcaacca tgctttcacc gtcaagccgc gctgggacct
     6121 ggccgaaatt tccgaataat tagcattgtt ggtccgtcgc gaggcagcgc ggggtcgccg
     6181 gaccgtcgag gcgggggcag ccccgcaggg gccgagggcg cctgatggcg cgcccgggcc
     6241 ccgccgacgg tccggcggcc ccgcgccaac tcccgacgga cggctcaaga acccaaatta
     6301 tttcttcttc gccccaatct tgctctcagc cccgccgacc agccgcgcaa tattggtctt
     6361 gtgccgataa ataagcagca aggcaatgat gacaaggctc gcgctcaccg gcgggtgcac
     6421 ggcccagccc agcttgccgc ccagcaggta atacagcggc gcgcagaccg ccgccaccag
     6481 cgcggccagc gacgaataac gggtcaccac cgccaccagc agccaggtca gcgccacggc
     6541 cagggccagc cagggctgca cggccagcag tacgcccagg gccgtcgcca cgcccttgcc
     6601 gcccttgaaa cccaggaaaa ccggatacag atgtcccagg aacaccgcca aagccgccag
     6661 cgcgtaaacc ggccaaggca gatcgggcac caggtgctgg accagccaga gcgcgaacca
     6721 gcccttggcg gcgtcgccca gcagcgtcag caccgccgcc actttgctgc ctgaacgcaa
     6781 cacattggtc gcgcccggat ttttcgaacc ataactgcgt ggatcctgca ggcccaggag
     6841 cttgctgacc accacggcaa agggaatcga accgatcaga taggccagca ggacgagcat
     6901 ggcgtaaaaa tagggcgagg aaacgtcgaa ggccatgggg cgggatcctg cgggttgggg
     6961 ataatcgtgg cagcggattc tacagcgcgg ccgggttgcc gatagggcat ggcacggcct
     7021 gcctgccgta caatcggttg ccccatgttg caggaggtcg ccggggcgac cggctcgcaa
     7081 cgacttcccg agcagaacag aaagatgcgc atcctggttt cgaacgatga cggttacaac
     7141 gccccgggga tcgaagcgct ggttcgcgcc ttgtccggac tgggcgagat cacggtggtc
     7201 gcgccggaga ccaatcacag cggcgcatcc aactccctca cgctgaaccg gccgctgtcg
     7261 gtgcgcaccg cttccaacgg gttttactac gtcaacggaa cgccttccga ctgcgtgcac
     7321 gtggcgttga cgggcggcct catgggcagc cgtcccgacc tggtggtctc gggcatcaac
     7381 aacggcgcca atatgggcga cgacacactg tattccggga cggtggccgc cgccagcgaa
     7441 ggctatctgt tcggcattcc cgccattgcc ttttccctgg ccgaaaaagg ctgggcgcat
     7501 atcgacgatg cggcccgcgt ggcgcgccag gtgaccgagc ggcaattggc caacccgctg
     7561 ggcgggcccg tgctgctcaa cgtcaacatt cccaatcgcc gcttcgagga catggccggc
     7621 tatgccgtga cgcggctggg caagcggcat ccgtccgagc ccgtggtgcg cagcaccacg
     7681 ccttatggcg acacggtgta ctggatcggc ccggccggtt cggcggccga tgccacgccc
     7741 ggcacggatt tccatgcgct ggcgcaaggc tgcgtggcgc tcacgccgct gcggctcgac
     7801 cttacccagt acgagcagct cgatgccgta cggggctggg tagaaaaatg atgaacgcgt
     7861 cgagccggct caagcgttcg ttccccctcg gggggagggc gcgcagcgcc aagggggtag
     7921 catgagcaag cgctttacgc ccgacgaact cgcccgccgg ggcgtggccg gcagccgcac
     7981 gcgccagggc acgaccggcg tcggcaaggg ctataccccc gccaacagca acacgcgtat
     8041 ttccgccggc gccatgccgc gccccaacca ggctccggcc atgtcgagcg ccgcgccggc
     8101 cggcggcaac ctgggcctga actccgacag gctgcgtcat gccatggtgc agcgcctgcg
     8161 cgcgcagggc atcgtcgacg agcgggtgct gggcgccatg tcggcggtgc cccggcatat
     8221 cttcgtcgac gaggcgctgg ccagccgcgc ctacgaagac gcggccctgc ccatcggcca
     8281 cggacagacc atctcgcaac cctgggtggt ggcgcacatg ctggccaccg tttgcgaaaa
     8341 ccgccagccc acgcgcgtgc tcgaagtcgg cgcgggctgc ggctaccaat cggccgtgct
     8401 ggcgcagttc gtgcgcgaga tctatgccat cgaacgcata cgcggtttgt atgagctggc
     8461 acgcgagcaa ctgcgcgcat tgcggcttgc atctcgtgtc agattgatac atggggatgg
     8521 catgctgggc cagcccggcg cggcgccttt cgatgctatt gttgtggcgg cggcgggact
     8581 ggccataccc caggccctgc tcgaacagct tgccctgggt ggtcgattga tcgcacccga
     8641 aggcggcgcc gagcagcggt tggtgttgat ggaacgcacc gggcccgcca gttggacccg
     8701 gcgcgaactc gaagcggtgc gcttcgtgcc tttgcgagcc ggaatacaat cttgagcaaa
     8761 caggagaatc gtatgctcaa cgggcagttt gcattgaatg acgcaatacc gcctggcgcg
     8821 tccgcgcgct tatcacgcca agttttaccg ctggtcgcct tgaccctgct ggccttggct
     8881 ggatgctcgt ccgaacctag tcggcaggca cccatcgtcg atctgaacga accctccagc
     8941 ggcctgtcgg ccggtggaac ctacgtcgtc aagcctggcg acagcctgta cggcattgcc
     9001 cggcgcaatg gcgtcgatat cgaaagcctc aagcgggcca acaacatcgc cgatcccaac
     9061 catctgtccg tcggccaggt gctgaagata ccgggcgccg gcggcagcgg ccccgtgccc
     9121 gtggcgcccg ccaccccgca acccacgccc gtgccgcccg cggccagcaa gccggccgag
     9181 ccggtgcccc tcgaccagct gccgccggcc agcggccagc cgcccgagcc gcctgccggt
     9241 accgcggccg tgcccgccgt gacggcgccg cccgcggcgg cgcgcgcgtc cgacgccaac
     9301 gtcatcaact ggggctggcc ggcgcaaggg cagatcagcc agccgttcaa cgccagcagc
     9361 aagggcatcg acattgccgg caacctcggc gacccgatca cgtccgccgc cgacggcaag
     9421 gtcatgtaca gcggcaatgg ggtgcgcggc ctgggcaacc tggtcatcat caaccactcg
     9481 aacggcttca tcacggccta tgcccacaac caggacttgc tggtcaagac ggggcaggac
     9541 gtcaagcgcg gcgccaagat cgccacgctc ggccagagcg atgccgcctc gcccctgctg
     9601 cacttcgaga tccgccgcca gggcacgccg gtcgacccgc tgcagtacct gccggcgcgt
     9661 tgagcgccga acggtcgcgg cctgagcatc catgacgccc accctggtct tcgatctcga
     9721 aaccctgccc gacgtcgtcg gcctgcggcg cctgaacggc tgggggccgg aggtgtccga
     9781 cctggaggtg gccgagcgcg ccttcgcggc gcgccgcgaa gccacgggcg gcgacttcct
     9841 gccgctgcac ctgcagcgcg tggcggtggt gggctgcgta ttccgcgacg accagggctt
     9901 tcgcgtcaag acgctgggca accccgatga tcccgagccc gtgctgctgg cgggcttttt
     9961 caagaccatc gaacgctata cgccgcggct ggtgagctgg aacggctctg gcttcgatct
    10021 gcccgtgctg cactaccgca gcctgatcca cggcgtgccg gcgccgcgct actgggacat
    10081 gggcgaagac gaccgcgact tcaaatacaa caactacatc agccgctatc acaaccgcca
    10141 cactgacctg atggacgtgc tggccaagta caacggccgc gccaacgcgc cgctggacga
    10201 cctggccaag ctctgcgggt ttcccggcaa gctcggcatg gacggcagca aggtctggga
    10261 ggcctggaac aacggccagg ccgatgcggt gcgcgcctat tgcgaaaccg acgtggtcaa
    10321 tacctggctg gtgtactgcc gcttccgctt cctgcgcggc gaactcgatg ccgccgccta
    10381 ccaggccgag atcgacctgg tgcgcgatac gctggccgcc agcggcgcgc cgcactggaa
    10441 ggaatacctg gcggcctggg acgcggcctg agcctggcgc ggtacgcctt cctggcaacc
    10501 gactgaaaca gccttcccag gggcgaaacc cacttgtaac tccccacgcc gcacaatgag
    10561 gccgtcgttc aacatcatta aggagacgct catgtcccgc accactcttc gcgcttccgt
    10621 cgccggcctg gccatggccc tggcctccgg cgccgcaatc cccgttttcg cggcgaccgc
    10681 cgccgcagcg tctccggcag ccggcgaggc tgcccagccg ccccagcacc cgggccattg
    10741 gcagcacggc gccaagggcc gcaagccgca tggcggcatg cccatgatga tgtggcgcga
    10801 aggcctgatg attcccggcc tgggcccgat cagcaagccg caggtcgagg cgctcaagct
    10861 cgacgccaac cagcaggccc tgctcaagca ggcgcgtgat gcccagggcg atctgttcaa
    10921 ggctcgccgt gaagccatgg tcaagcaacg cggcctgatc gaccagcaaa cgacggcagg
    10981 caagctcgat ccccgcgccc tggccagcgc ggccgacgcc aaccgcgatc aattccgcgg
    11041 ccaggctacc cagctgcgcg acaagtggct ggccgtctgg gacagcctga acgacacgca
    11101 gcgcacccag gtggcgggtt tcgtgaagga gcgcgaggcc aggatgaatg acatgcatga
    11161 gcgcatggaa cacaaaggcc attccaagca gcaaggccct gccgcgggct gatcgcccct
    11221 tcgttcccgg ttccctttgc caagccggct caggccggcc ctcgagagcc cccgatgtcc
    11281 ccctgggacg gcgggggctt ttttacgtac ggccggtcag ttgacgagcc tggacccaaa
    11341 agtatttgaa aaaatactta ccatgtggaa ataaatactt gaagcctatg cggtttaggc
    11401 ttcaagtatc accttattgg tgatcgatcg cttcaagttt ggtgcgtaaa agaaaaaatg
    11461 caccaaattg gggctttcga atcgattcaa ttctgaaatt cttctttcaa ttttgaaaga
    11521 agaagaactg gcacgaaaaa tgctcagaga agtaagccac ccctgacgtg cgaacgcagg
    11581 agggcccaat cggacgcgca gtgggagaca agcctgcgcg tccatcgagc atatcaacca
    11641 ggaatcgacc acatgaaaaa gaagttgctt gctttgcccc tggcatttgg cgcctgtttt
    11701 ggctccgcgg ctcacgccgc cgatggtacg gatctcggcg gcgggttttc cctgacgggc
    11761 aatgccgccc tggtcagcga ttaccgtttc cggggctatt cgcagaccaa tatgctgcct
    11821 gctgtgcagg ggggtatcga cctgacccac agttccggtt tctacctggg taactgggac
    11881 tcgagcattt ccgacaaggt cttcaccgac ggcgtcgtcg aaatggactt ctacggcggc
    11941 tggaagggcg atatcggcca cggcctcggc ctggacgtcg gcgggctgga atacgtctac
    12001 cccggctcca cctcgcccaa gggcaacatc tacaacaaca ccgacctcta catgggcctg
    12061 agctacgacg cctatacgct gaagttctcc tacacgccca ccgacttctt cagcgtgccg
    12121 aactccaaga acacctggta catcgacggc tcggccaact gggacctggg cagcggctgg
    12181 gggctgaacg cccacgtcgg ctaccagaag ctgatgggca acgccaggtt cggcgccgac
    12241 ggcaacacct actcgggcat cgtcgactac aagctcggcg tcaccaagga cttgagcggc
    12301 tgggtgctgg gcctggccat ggtcggcacc gaccaccagc gctacatcgt gaccccgcat
    12361 ggcgcggccg gccgctttgg cgtggtggcc tcggtggccc acaccttcta agcgggtcga
    12421 ccgtttgcga ggggcgcacc tagcggtagg cgcgcccctt tgcatctctt ttacaatggc
    12481 gggctgtcta tctggatggc cgacatgccg gaagtcttga atatcgaatc cctggacctc
    12541 gaagccaggg gaattgcccg ccgcgatggc aaggtcattt tcgtagaggg cgccttgccg
    12601 ggcgaacggg tgaccgtgtc caccgtgcgc cgcaaaccgt cctacgagat cgcgcgggtc
    12661 gacgaagtgc tgtcgccctc gtcgcagcgc gtcgagccgc gctgcccgca tttcagcgtg
    12721 tgcggcggct gcgccatgca gcacctggag ccctcggccc aggtggccat caagcagcgg
    12781 gcgctggaag acaccttcgt gcacatcggc aagctcaagc cggcgcgcat gctgccgccg
    12841 ctgcatggcc ccacctgggg ctatcgctac cgcgcccgcc tgtcggtgcg cgtggtgccc
    12901 aagaagggcg gcgtgctggt gggttttcgc gaaaagaaaa gcagctacgt ggtcgacatg
    12961 cgcgaatgcc atgtgctgcc caggcatgtc agcgacctgc tgctgccgct gcgcgccatg
    13021 atcgccgcca tgtccgcgcc cgcccgcctg ccgcagatcg aggtggccct gggcgatgcg
    13081 gccaccgtgc tggtgctgcg tcacctggag ccgctcaccg gtggcgacat cgccgtgctg
    13141 cgcgcctttg ccgccgagca tggcgtgcag tggtggctgc agcccaaggg accggacacg
    13201 gtgcatgcgc tcgaacccga acatgccgat gcgctggcct acgcgttgcc ggaattcggg
    13261 ctgcgcatgc cttaccggcc cacggacttc acgcaggtca accatgccat caaccgcgcg
    13321 ctgatctcgc gggcgctgac gctcttgggc gtgcagccca ccgaccgtgt ggcggatctg
    13381 ttctgcgggc tgggcaactt caccctgccg ctggccacg
//
LOCUS       DASWPO010000341        26766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2315221, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000341 DASWPO010000000
VERSION     DASWPO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 26766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26766
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315221"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1276)
                     /locus_tag="VGC69_18100"
     CDS             complement(53..1276)
                     /locus_tag="VGC69_18100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0297245.1"
                     /translation="MLNSVSILIAVMSLANLMSVAIFGSLRHAGIPGVGRWMAANTMA
                     FAALILFALRDVSPVFLTVVVGNALFSLSSVLGYEGCRQFFRLPPVTVAPYVGCVAVT
                     AGIVYWTYVSPDIDIRVVIVSIFFGCTYGLMGWTIWRGWKPGRSFYCYGFVLAISGIA
                     VLASAARALVFGVGLAQQQTLLLPSSLNIAFLMLGMLALPGVSSGMVMLAHDRMAERL
                     ERWANLDELTGALTRRGFFARMRVMVERTRHAGGVLSAAVVDLDHFKSINDLYGHATG
                     DRVLAHVGQLVASTLAEDEVFGRLGGEEFALLFPGVGKQEALARLESLREVLLASLAQ
                     EPAGTDAALPGFTFSAGMDEYRHGESPESLMLRADTALYAAKARGRDRIVAADAAPDA
                     PGNQAALAADYAVRR"
     regulatory      1422..1492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1583..2950
                     /locus_tag="VGC69_18105"
     CDS             1583..2950
                     /locus_tag="VGC69_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEY0297246.1"
                     /translation="MPKPSRIPEQLELLPTLGKWLVIASATAALAGSASALFLLALDA
                     ATAWREAHPRVIWLLPVAGFAVGLAYHLLGKPVDAGNNLIIDEIHDPKKIVPLRMVPL
                     VLGGTVLSHLFGASVGREGTAVQMGGALADQLTHVLRLKHEDRRILLMAGISAGFASV
                     FGTPLAGAVFGLEILAIGRMRYDALFPCVVAAIVADQVCLAWGIRHTHYAIGEIVPIG
                     TWSVLAVVIAGMVFGVAGMTFANATHKLGAWVKQHIAYAPLRPLAGGIVIAAAVWALD
                     AYHYIGLGIPDIVRSFQEPMKPWDFLGKFLFTVASLGTGFKGGEVTPLFYIGATLGNA
                     LAPILHLPFAMLAGIGFVAVFAGAANTPIATTLMAMELFGAGIGPLAAVGCITAYLFS
                     GHTGIYPAQRVGNKHRKHRLAVPEGLRIADLAHFRKQKREQEKKAPSPRRTRALDVPP
                     GPGIP"
     gene            3061..4128
                     /locus_tag="VGC69_18110"
     CDS             3061..4128
                     /locus_tag="VGC69_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEY0297247.1"
                     /translation="MKVAILGGGHGCYAAAADFTRRGHEVRMWRRQAAQRVAGYPIAM
                     RDKQGEHQLAIALYTPDIGEAVREAELIVVPTPAFAQEDIARQLAPHLRDGQVVFLPP
                     GTFGSLVMWRALRQSGNQAEVAFCETGTLPYLARKHGEQQVTITSYATRLPTGVFPAR
                     LAAHAFAVLCQGYPVEPAEDALSGALMNAGPIIHPPLILMNAGPLQHFPAWDIHNEGT
                     QPAIRAVTDRLDEERIRIREKLGYQAPHFPLRDHYANDLWMYGDAHQRLVDSGDWREN
                     IDLHTHRYMTEDVHYGLAFLVSVANWCGASAPIASGLLAIAGAIGGQDLNLGPRTLSC
                     LGLASMTPGELKQKLVTGEGL"
     gene            4125..5123
                     /locus_tag="VGC69_18115"
     CDS             4125..5123
                     /locus_tag="VGC69_18115"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY0297248.1"
                     /translation="MSAPAALAPHVAAFGAGRMGRSIAAAYALAGQDFTLIDLRVRTD
                     QAWRRLQAEVREELQDSFARLLSLGCIDQATAQAAPGRVTLAGRGDAPAALAASTLLF
                     EAVPETLDAKREALEYAGRHAAPGAVIASTSSTMLAGELASLVPHPERFLNAHWLNPA
                     YLIPLVEVSPHAGTAPGALEQTLASLKAAGKVPVVCNDKPGYIVPRLQTLVMNEAARM
                     IEEGTATAADIDRAIRYGFGPRYAAMGVVEFIDFGGLDILYHASRYLSTRLDPSRYAC
                     PQIIEDKMQAGELGMKTGQGLYAWDAQQARHFQQDALARLVRVLALDTQAPAPRTK"
     gene            5189..6172
                     /locus_tag="VGC69_18120"
     CDS             5189..6172
                     /locus_tag="VGC69_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297249.1"
                     /translation="MAFKPMKYVLSAVLAGLSCAALAQDVPTLRVGWTIPAEEAKYWM
                     MRRPEAFPDLGKTYKIEWTQFQGTAPMVQAMISDALDCSSQAPLSLGQGAVNGGLSAY
                     IVAQHVGESPSSFSVYWGVLDGSPIKRVEDLKGKTVGVNVYGSGVYGQMAAILKKHGL
                     DPAKDVKLVETGFPGSESALRGGRVDAAVFVQPFAARAEAKGGIHKLFSMQELMPNTV
                     QIVEVCRKDVAGKSPELIKSYVRDLTRAMKMALAHRDDTVKIDSEVTRAPVEALDTYL
                     LKPNDFAHDPGAHPDFDAIQAMFDQYAALGLLSRPLKVSDFRRDDLVAPLQ"
     gene            6183..6971
                     /locus_tag="VGC69_18125"
     CDS             6183..6971
                     /locus_tag="VGC69_18125"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297250.1"
                     /translation="MSKRLIEIAGLSKRFDVPGRPAHLALDGIDLGIGEGEFVSLLGP
                     SGCGKSTLLYMVGGFIEPSDGEIRVDGRPVRGPGPDRGPVFQEFALFPWKTVLQNVMY
                     GLRVQKVPAPQARQRAEALLRKVGLADYAGFYPKQLSGGMRQRVAIARTLAYEPRILL
                     MDEPFGALDAHTRTTLQNELLAIWERDRKTVLFVTHGVDEAVYLSDRVVVMSGSPGSV
                     HRIVDIDLPRPRVRAQLLTDPRYQQYVLELEAMLDHARVPEAAA"
     gene            6968..7747
                     /locus_tag="VGC69_18130"
     CDS             6968..7747
                     /locus_tag="VGC69_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297251.1"
                     /translation="MKRRMGWRRGWRALLACAGLLLAWQAAALWLKMEELPSAWETLR
                     AVPAILSNGEDLLSILASLRRMALGFGLGLLIAVPLGLAMGRSKPLARLLNPLTSLAY
                     PIPKAALMPIIMLWFGIGDLSKVLVIFMGVSLPLLYHSSQGAEQVDDKLVWSAQAMGM
                     GRLARLARVILPAALPEVMLGCRVAVSMALITMISSEMIARRNGAGDLLFNAMDMAVY
                     TDVYAVMLIIAAIGVALDVLVERLRAALTHWADTLQPGSAG"
     gene            7744..8487
                     /locus_tag="VGC69_18135"
     CDS             7744..8487
                     /locus_tag="VGC69_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297252.1"
                     /translation="MKRPSRSYGAAWPVIGLLLVWQLVCASGLVPPALLPAPLTIALR
                     LAQELLEPGFLFHIGQTAIRLFAGFFIALALGLGLGIAMAQGAEPLLTPLVRVAAPIP
                     KIALYPALVLLLGFGHASKIALVVADAVFPLMLATLHGLRSVQPKLVWAAQAAGASRS
                     AIVLTVMLPSALGPILAGCRIGLVIACVNVFLAEMISSSDGLGHLLVTAARSYQVVDM
                     FVPLVLISLIGLALNASLQFARHRFAAAL"
     gene            8491..8940
                     /locus_tag="VGC69_18140"
     CDS             8491..8940
                     /locus_tag="VGC69_18140"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0297253.1"
                     /translation="MPASAYPGHDQFLPYLINRTATQINRRLLDILDQHGLTMTHWRV
                     LAFLSRQDDLSIGELAQATMTEQSTLSRSLRALEARGYVRRRASSTDTRAVHVHLEPQ
                     GRKAFESMLAPALELEAVYMEGISDRDRQTLRKLLQRVLQNCEGRAA"
     gene            9038..10207
                     /locus_tag="VGC69_18145"
     CDS             9038..10207
                     /locus_tag="VGC69_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY0297254.1"
                     /translation="MLSRALDNVRVLDLSRILAGPWCTQNLADLGADVIKIERPGQGD
                     DTRKWGPPYLRSADGSDAMAAYFTCCNRNKRSVCLDFSSQPDRKLLLDLVRSSDVLVE
                     NYKTGTLKRYGFDYDSLKAIRPDLIYLSITGYGQNGPKASQPGYDYIFQGIGGLMGYT
                     GRADGEAGAGPLRAGIPVVDISTGMYATSAVLAALLQRARTGKGAYLDIALLDVAVAI
                     NANQGANYLVSGKNPERSGNAHPNLAPYEVFKARDGYFILAIGNDGQFLKLCELCGHP
                     ELAADPRFSTNSARIGNLPALRGILGDLIALHDRSHWTSSLEAAGIAWGPVNTLDEVF
                     NDEQVRHRGMLRHAPHPRFGEIPSVRNPMLTGEDTAPMRPPPLMGEHTQEVLDSL"
     gene            10244..11200
                     /locus_tag="VGC69_18150"
     CDS             10244..11200
                     /locus_tag="VGC69_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297255.1"
                     /translation="MNAALTALVFGTASACALAGYPDHNVTLVVPYAPGGTTDLMARA
                     VAPKLGEVLGQAVVVINKPGAGGELGSAYAARERPDGYTLVMDVESSHAVIPNVYKKP
                     SYDPVKDFSPISNLANVLGVLDVRADSEIKTFGQLVSVLKANPGKYSFGSSGAGGYSH
                     LFGELFLSSTGTQMLHVPYKGLGPALVDLMSGQVQVVFDNLPSSAGFLQGGKLRALAV
                     ASPARIKEMPDVPTYAEVGYPQLNTPSWFGLAAPAGTPEPILDQLNLAVKKVLTDPRV
                     IEIIQKAGAVPDYTTREQFSEMIKHSNAVWKKVVDDIHFEKL"
     gene            11215..12000
                     /locus_tag="VGC69_18155"
     CDS             11215..12000
                     /locus_tag="VGC69_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0297256.1"
                     /translation="MTASFHAPHADFTLKANGVGMLNICDAGKLNILGTPVVNGLIDA
                     LARIGQQTRIKVLVLRAEQEKAFVAGSDIKEMAHFDRSSAIAYIDRLRHLCELVRTFP
                     MPVIARIPGWCLGGGMELALSCDLRIASDQAHMGMPEVKVGVPSIIHAALLPRLVGKA
                     RANWMLLTGEIVDAPQALTWGLLDRVVSADSLDAEVDRIADLLAGLGAGVLAQQKRLL
                     REWEDEPLNISIQHGVLEFGAAFDTGEPGRYMRAFMEGKARKE"
     gene            12055..12999
                     /locus_tag="VGC69_18160"
     CDS             12055..12999
                     /locus_tag="VGC69_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0297257.1"
                     /translation="MSYEWKDLHLFKAIIQLGNLSAAAAAMHMTAPSASYRIKNLEYT
                     AGSPLFVRSAKGMRLTAAGEALARHVDILLAGLHAMQAEISAYAHNLKGNIRLLANSS
                     SLNGFIIPSLARFLASNLHIDVDLKEQDSPSIAGKILRGEADIGICSGKVDDPDLQCE
                     LYAIDRLVCVVHQEHALAQRDRSRFSEILDHGLVCMARSSSNFQFVLEQAERAGKPAN
                     VRVHAHDFNSVLYLVRSGVGAALVPASVAQPFVREGALNSITLLDAWALRDLHLITRS
                     DSDPAGLVRQFADVLLHDPQVAAARSAGVTPGWHGDIG"
     gene            complement(13010..13861)
                     /locus_tag="VGC69_18165"
     CDS             complement(13010..13861)
                     /locus_tag="VGC69_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY0297258.1"
                     /translation="MHLLQILILAIVQGAAELLPVSSSAHVIVAEKLMGLDPTAPAMT
                     MLLVMLHTGTMFAAIVYFWKAWIATYFSSFSAFRANVLRLVVATAATGVVGLALLFII
                     KHLVYRHASSFEIEHLFGNATLMAVSLAAAGVLIILASRIRERSGDLTLRDSLWIGAV
                     QGLCLPFRGFSRSGATISTGLALGVGRRRAEEFSFALAVVLTPPVIVKEAWRLYQDAH
                     AQAQTAGAQAVDYAHLLGPSLIGMVFSFLAGLVALRWLSKWLEQGRWSFFGIYCLLAA
                     VVVLLVG"
     gene            complement(13964..14962)
                     /locus_tag="VGC69_18170"
     CDS             complement(13964..14962)
                     /locus_tag="VGC69_18170"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0297259.1"
                     /translation="MNSPGSSPAMSSRPAWRHWQAEGSALQRVDYWKDVVCQAVLHVT
                     MTPDEPGDPLLFDGVIQSRDQAGSRLVNFRSSAHRIIRNARQVNQGDDQYLMISLQCR
                     GASHMEQRKGGVSLGGGDIGVIDSAQPFQLHFPDAIERRIVMLPRALLAARLPRFGML
                     DEPLRLGADDAVTSVMAHTMQVLTGPAEVGDADVQVLLANIADLLALRLRGARGANLP
                     GRMAYEQLLRAIDRNLDVIGFTPAEAAARTRISLRTAHRLFQQYSEPRISLEQYIVDQ
                     RLARAHGALTQGRAASVSEAAFAAGFSDLSHFARRFKARFGVAPSSLLLRDSPRAR"
     gene            15197..16732
                     /locus_tag="VGC69_18175"
     CDS             15197..16732
                     /locus_tag="VGC69_18175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0297260.1"
                     /translation="MSHQPPSDALAAFVTQVRAELGEDAITLADETLARYGEHTLPAP
                     DQPPGAVAYPACTQDVQKLVLAANQHKIPLFPISAGQNIGLGSKSPVRPGQVIVDLGR
                     HMNRVIEVNDTLGYCVVEPGVSFQAMHDELQRRGSKLMMSPTAGPPQGSLLGNALDKG
                     GGGGAAGDHFGMSCGMEIVLGNGEIIRTGDGSLDSPEQLNWHVSKYSFGPALDGLFTQ
                     SNYGIVTRMGMWMMPRPPHIEPFFFTFPDDDDLGLIIDLIRPLKQTNFVPTQIRATND
                     LYLLSSAASHPEYAATRGKEQISDAARRELQRSFGLGAWTVSGAFYGASAQALQPQLE
                     RVRAHFTQGGKGRHIPPEEAADIAPLHIARLNYAGVPGEGELRMLRWRPGGGTSWFLP
                     GTPMLGDVANAFQKRSRAICKEYGLEYMVSNVCGPRFARGVHTLVYNRQDPDESARAD
                     ACYRALAASFAERGVFVGRAPTLYQAMHQDHRLPAFNQACAAIKRALDPNGIIAPGKY
                     GIE"
     gene            16773..17744
                     /locus_tag="VGC69_18180"
     CDS             16773..17744
                     /locus_tag="VGC69_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297261.1"
                     /translation="MRKSLAAISLAIAGTLSMFHAACRAQDYPARQVRLEVGFPPGGG
                     NDLLARLLGAELERALGQPFIVENRPGAQGFIAFNTVKTANPDGYTLLIGPSSGMTVN
                     PEVYRTLPYDAIKDFAPISLIGAFPLIVTAAPKAPFGTLKALISAAKSQPGTINYASA
                     ASSFQLATEMFAQQAGINLHNIPYKGSAQSVQAVMGNQVPLTFADSAAVIAEIKSGAL
                     RALAVTSKTRVPTLPEVPTVAEAGLPGYEMVLWSGLFAPAGTPRDIVDTLQRAVAQAI
                     HAPQMQEKLQLLGVMPVGSTADELADTMQRQISEYTRVAKTANITVD"
     gene            complement(17885..19429)
                     /locus_tag="VGC69_18185"
     CDS             complement(17885..19429)
                     /locus_tag="VGC69_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0297262.1"
                     /translation="MRINEPVTQREFEFPDHATLMSTTDTRSHIAYANAAFIQVSGFS
                     PQEINGQPHNMVRHPDMPKEAFADMWNTLKGGEPWTALVKNRRRNGDHYWVRANAVPV
                     VRRGQTTGYMSVRTKPSRAEIDGAEALYKTFREGRQGSKRFHKGLVVRTGLLRWRSVF
                     QTLSVRGRLRTAMLPLIPLFILGGWQAGLAGPNLAAFGGGVAVLTLLALFWLQRQISR
                     PLEFVLEQALQVASGASTSVEHMNRVDEIGMTLRAIGQLGLMFRWLIDDVSEQVVNVQ
                     TASNEIAQGNNDLSSRTEAAATSVQQTAASMTQMTATVKSNAETAQQANMLSGTAREA
                     ATKGGTAVTEVIKTMDDITDSSRKITNIISVIEGIAFQTNILALNAAVEAARAGEQGK
                     GFAVVAGEVRTLAQRSAQAAKEIKQLIEDSADKIGAGSSLVNDAGKTMVEIVTQVQRV
                     SDLIAEISSATEQQSAGVTQVDQAVGSLDEITQQNAALVEQSTAASESLRQQATRLVE
                     AVSVFR"
     gene            19813..20250
                     /locus_tag="VGC69_18190"
     CDS             19813..20250
                     /locus_tag="VGC69_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297263.1"
                     /translation="MAYASLGAMAALFGRFASPADRRRIVGLCGIWLTLAVLSMSAAS
                     FLGIDPTLLLLLLALSCGFFFFVVSSSGFGPPGALIFVFAVGASMSRAESWHAVFERT
                     AATAAVVRRLHRLGDPEAGAVGLGRVVLARGPRPRRVSGIAGA"
     gene            complement(20228..21607)
                     /locus_tag="VGC69_18195"
     CDS             complement(20228..21607)
                     /locus_tag="VGC69_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297264.1"
                     /translation="MLLSFARTQVYPSGQGVPDAERLSAVLSVVLGVSMSSLDTAIAN
                     TALPTMAHDLGTTESLSVWIVSAYQLTMAAGLLPFAGLGEIVGYRRVMMTGLVLFTLA
                     SLLCGLSSSLEWLVIARVLQGLGGSALMSANGALVRFIYPSNQMGRGAGINALVVGVS
                     FAAGPTIASLILSFASWHWLFLVNVPLGLLAIYLGLRALPATPRSQQRFDHLAGLLCS
                     VFFALLIFTLNEAAHGQGWGRVGGEAVLTLLCLWWLLRRQAGMTAPVLAVDLLRRPMF
                     ALSALTGVCSFATQALAFVSLPFMFQHVLGYTQIETGFLITPWSVVVALAAPIAGRLS
                     DRYSSGMLGGVGLALLCLGMVSMALMPAHPSVLSIAWRMMLCGAGFGFFQSPNIRAIM
                     TSAPPERSGGASGMVGTVRLLGQSTGAALVAACLHVSTTHGAVAALWLGAVFAGLASM
                     ASFMRLQYR"
     gene            21705..22553
                     /locus_tag="VGC69_18200"
     CDS             21705..22553
                     /locus_tag="VGC69_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0297265.1"
                     /translation="MKLVRYGSPGQEKPGLIDAQGALRDLSGHLADIDGAALSPTVLA
                     ALAGIAPDSLPRVDGAVRYGCPVAGIGKIVCIGLNYADHAAEAGLAVPTEPVVFLKPS
                     SSLSGPNDPVTIPRGSVKTDWEVELGVVIGRKASYVEESEALGYVAGYTLVNDVSERE
                     YQIERGGQWDKGKGCDTFCPVGPWLVTADAVPDPQALPLWLEVNGKRYQDGNTRTMVF
                     GVKTLVSYVSRFMSLMPGDIISTGTPPGVGMGQKPPVFLKAGDVMRLGIDGLGEQRQA
                     LVDWKR"
     gene            22590..24176
                     /locus_tag="VGC69_18205"
     CDS             22590..24176
                     /locus_tag="VGC69_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY0297266.1"
                     /translation="MKRRAFLVHTLAGGALLLARPASAQPAGHAMHGMSGMQGMEGME
                     GMAPAPATAAKLAADGALPAGAPLAALPALANESRQPGLFRATLAAQPARQALMQGKA
                     TLMWQYAARAGGRPAGIAGPLIDAREGDTLEIRFENHLPQPSTIHWHGLPVPPEQDGN
                     PADAVAPGAARTYRYTLPPGSAGTYWYHPHPHMRTAEQVFRGLAGPIIVRAADDPLAG
                     WPERHLFISDLRLAADGSIPANDMNDWINGREGQFVLINGALRPEIAINGDERWRVWN
                     ACSARYLKLAFSDGRAFTQVGTDGGLLERPVEGLKELLLAPGERAELIVRAGGQASQA
                     ALMALAYDRGKMNMVGDLPPETGRTLAQVTFAPAAGDTARPLPATLRAIAPLGAPAAK
                     KTVMLTEQMDMQQDMHATNLQGMPDGMRFLINGASFDPQRVNLTSRRDQVEVWTLQNF
                     TDMDHPFHIHGTQFQVLDRRHEGATTAEPCLAWRDTVNLRPSETVRIATVQRSAGQRM
                     FHCHILEHEDLGMMGLLQVV"
     gene            complement(24202..25608)
                     /locus_tag="VGC69_18210"
     CDS             complement(24202..25608)
                     /locus_tag="VGC69_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0297267.1"
                     /translation="MTTRPLWQWGAAELAEAILRREISSRQAVDSAFARLDAVNPHVN
                     AVVDVLYDDARQAADRADAAVRHGQPIGALHGVPVTVKIDTDYAGRPTTRGVVAYRDQ
                     IAPEDAPAIANLRRAGAVIIGRTNVPAFTARYFTDNDLHGRTYNPWNRAVTPGGSSGG
                     AAVATAVGIGALAHGTDRSGSIRYPAYVNGVYGLRPSFGRVPLYNATAQSSPGLTSQI
                     TSVAGLFARRAADLPLGLAALAQPDPRDPWHVPAPLVAQEAPRLKVGVAAQVDGYETD
                     PAVAQAIARTRAALQDAGYEVEDLELPGFAEAARLFRQLASAEEALDRLKAIEAHGDE
                     AVRTARRNARGSVPALDFEGYIQGLGRRHALAREWNLLLRDHPLLLLPLSFRRPVAVD
                     HDQGGAAQMHRFLQDQHPLLAVALLGLPALSLPAGEADGVPVGVQLVAGRFREDVLIR
                     AAQAIEPAQHFATPIDPR"
     gene            complement(25701..>26766)
                     /locus_tag="VGC69_18215"
     CDS             complement(25701..>26766)
                     /locus_tag="VGC69_18215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297268.1"
                     /translation="SGAMMVPVGRLIMLRSVSKSQLIEAMAWVLMPAMIGPMLGPPLG
                     GFITTYYSWHWIFFINVPIGLLGLVMALIHVPPLRMDKPARLDVRGLLLMGVALAATM
                     YGLDLFSRDSADWTRVGAFLAVAAVSGVAYGVHARRCAHPVLDFSLVRIQSFSVALVG
                     GTLARIGFGALPFLLPLMLQLGMGLNAMQSGLAMLASGIAALGAKGASVGVLRKFGYR
                     NVLLWNGVACTVSLALCAVFRPGWPIPAIAAVLLLGGMARSLQYNVYGTFAYADVPSA
                     RMSAATSLNSTFQQLSATLGVAFSVCVLHLSMQWSQRQAPVRGDFILAFTAVALVTAL
                     AWPLCYRLPADSGRALMGGR"
BASE COUNT         4647 a         9348 c         8636 g         4135 t
ORIGIN      
        1 gacaggtgaa ccacgcgtac acgcaagacc tgttgcgggc cgcgcaaggc tatcagcggc
       61 ggacggcgta atcggcggcg agggcggcct ggttgccggg agcgtccggg gcggcatcgg
      121 cggccacgat ccggtcccgg ccccgcgcct tggccgcgta caacgccgtg tccgcccgca
      181 gcatcaggct ctcgggcgac tcgccatggc ggtattcatc catgcccgcg ctgaaggtaa
      241 agccgggcag cgcagcgtcc gtgccggccg gctcctgcgc caggctggcc agcaggacct
      301 cgcgcaggga ctccagccgc gccagcgcct cttgtttgcc gacgcccgga aacaggaggg
      361 cgaactcttc gccgcccagc cggccgaaca cctcgtcctc ggccagggtg gacgcgacga
      421 gctggccgac atgcgccagc accctgtcgc ccgtcgcgtg cccgtaaaga tcgttgatgg
      481 acttgaaatg gtccaggtcg acgacggccg ccgacaagac gccgccggca tggcgcgtcc
      541 gctcgaccat cacgcgcatc ctggcgaaga agccgcgccg ggtcagcgcc ccggtcagtt
      601 cgtccaggtt ggcccagcgc tccaggcgct cggccatgcg gtcgtgcgca agcatgacca
      661 tgccgctcga taccccgggc agggccagca ttcccagcat gaggaatgcg atattcaagg
      721 atgaaggcag cagcagggtc tgctgctgcg ccaggccgac gccgaaaaca agcgcccggg
      781 ccgcgctggc cagcacggcg atgccggaga tggccagaac gaagccatag cagtagaaag
      841 accgccctgg tttccagccc ctccagatgg tccagcccat aaggccgtac gtgcagccaa
      901 agaagatcga cacgatgacg acgcgaatgt cgatgtccgg cgagacataa gtccagtaga
      961 cgatgccggc cgtgacggcc acgcagccga cataaggcgc cacggtgacg ggcggcagcc
     1021 ggaagaactg ccggcacccc tcatagccca gcacgctgga aagcgaaaac agcgcattgc
     1081 ccacgaccac cgtcaggaac accggactga catcccgcag ggcgaacagg atcagcgcgg
     1141 cgaaggccat cgtattcgcc gccatccagc ggccgacgcc cggtatgccc gcatggcgca
     1201 gcgacccgaa gatcgccacg ctcatcagat tggcgagcga catgacggca atcaggatgc
     1261 tgacggagtt gagcatggcc gagggaatgg ctccggcttc gaagaagctt tataccgtat
     1321 ccaactgcca cacggcgtgc tccctggaaa gcgctggttt acggtgccgc ggccctgttg
     1381 cgcgggccgg catcgacgat gcgaacccga tcctgctata ttgccaaaca ggagatggcg
     1441 ttcctcccct gaacgaaacc gcagccctgc tgccgatgac gcctacagac acctgacctt
     1501 tgtcggggct gtgggcacgc gcatcgttct tccgcagcta catccccgca ttggtcagcc
     1561 cgaagatccg agcaaacgac cgatgccaaa accctcccgc attcccgaac aactcgagct
     1621 attgccaacc ctgggcaaat ggctcgtcat cgccagcgcc actgccgcct tggccggcag
     1681 cgcgtcggcg cttttccttc ttgccctcga cgcggcgacc gcctggcgcg aggcccaccc
     1741 ccgggtgatc tggctgctgc ccgtcgcggg cttcgcggtg ggccttgcct accacctgct
     1801 gggcaagccg gtcgacgccg gcaacaatct catcatcgac gagatccacg atcccaagaa
     1861 gatcgtgccc ctgcggatgg tgcccctggt cctgggcggc acggtgttgt cgcatctttt
     1921 cggcgcctcc gtgggccgcg aaggaaccgc cgtgcaaatg ggcggcgcgc tggccgacca
     1981 gctgacgcat gtcttgcgcc tgaagcatga agaccgccgc atcctgctga tggccggcat
     2041 cagcgccggc ttcgcgtccg tgttcggcac gccgctggcc ggcgcggtgt tcggcctgga
     2101 gatcctggcc atcggccgca tgcgctacga cgcgctgttt ccctgcgtgg tggccgccat
     2161 cgtggccgac caggtatgcc tggcctgggg catccgccac acgcactatg ccatcggcga
     2221 gatcgtgccc atcggaacct ggagcgtgct ggccgtcgtc atcgccggca tggtcttcgg
     2281 cgtggccggc atgaccttcg ccaacgccac gcacaagctg ggggcctggg tgaaacagca
     2341 tatcgcctac gcgccgctgc gccccttggc gggcggcatc gtcatcgccg ccgcggtatg
     2401 ggcgctcgat gcctatcact acatcggcct gggcatcccc gacatcgtgc gatctttcca
     2461 ggagccgatg aaaccctggg atttcctggg caagttcctg ttcaccgtgg cttcgctggg
     2521 cacgggcttc aagggaggag aggtcacgcc gctgttctat atcggcgcga ccctgggcaa
     2581 tgcgctggcg ccgatcctgc acctgccctt cgccatgctg gccggcatcg gattcgtcgc
     2641 ggtgttcgcc ggcgccgcga acacgcccat cgccaccacg ctcatggcca tggagctgtt
     2701 tggcgccggc atcgggccgc tggctgccgt cggctgcatc accgcctacc tgttctcggg
     2761 ccataccggc atctaccccg cccaacgcgt gggcaacaag catcgcaagc accggcttgc
     2821 cgtcccggaa gggctgcgca tcgccgacct ggcgcatttc cgcaagcaga agcgcgaaca
     2881 ggagaaaaaa gcacccagcc cgcgcaggac ccgcgccctt gatgtcccgc cagggcctgg
     2941 tattccctag ctggctcgtt cccgccggcc agcctattct cgatatatat gcatgtgcat
     3001 gcatgttgag agatgggctg gatgcttccg gcctgcccag cccacccaag ggaacccatc
     3061 atgaaagtag cgatactcgg cggcggccac ggctgctatg cggcggcggc ggatttcacc
     3121 aggcgcgggc acgaagtccg catgtggcgc cgccaagccg cgcagcgcgt ggccggctac
     3181 cccatcgcca tgcgggacaa gcagggcgag caccagcttg ccatcgcgct ctacacgccc
     3241 gacatcggcg aggccgtgcg cgaggccgaa ctgatcgtgg tgcccacgcc ggcgttcgcc
     3301 caggaagata tcgcgcgcca gctggcgccc catctgcggg acggccaggt ggtgttcctg
     3361 ccgcccggca ccttcggcag cctggtgatg tggcgcgcgc tgcgccagtc cggcaaccag
     3421 gccgaggtgg ccttctgcga gaccggcacc ctgccctacc tggcgcgcaa gcacggcgag
     3481 cagcaggtca ccatcaccag ctatgccacg cgcctgccta ccggcgtgtt cccggcccgc
     3541 ctggccgccc atgccttcgc ggtgctgtgc cagggctacc ccgtggaacc cgcggaagac
     3601 gcgctgtccg gcgcgctgat gaacgccggc cccatcatcc acccgccgct catcctgatg
     3661 aacgccggcc ccctgcagca cttcccggcc tgggacatcc acaacgaagg cacgcagccg
     3721 gccatccgcg ccgtgaccga ccggctcgac gaggaacgca tccgcatccg cgagaagctg
     3781 ggctaccagg cgccgcactt cccgctgcgc gaccactatg ccaacgacct gtggatgtac
     3841 ggcgacgcgc accagcgcct ggtcgacagc ggcgactggc gcgagaacat cgacctgcac
     3901 acgcaccgtt acatgaccga ggacgtgcat tacggcctgg ccttcctggt gtcggtggcc
     3961 aactggtgcg gcgcgtccgc gccgatcgcc agcggcctgc tcgccatcgc cggcgccatc
     4021 ggcggccagg atctcaatct cggtccgcgc acgctgtcct gcctgggcct ggcgtcgatg
     4081 acgcccggcg aactcaagca gaagctcgtc accggggaag ggttgtgagc gcgcccgccg
     4141 cgcttgcccc gcacgttgcc gccttcggcg cgggccgcat gggccgcagc atcgcggcgg
     4201 cctacgccct ggccgggcag gacttcaccc tgatcgacct gcgcgtgcgc acggaccaag
     4261 cctggcggcg cctgcaggcc gaggtgcgcg aggaactgca ggacagcttc gcccggctgc
     4321 tgtcgctggg ctgcatcgac caggccacgg cgcaagcggc ccccggccgg gtaacgctgg
     4381 cgggccgcgg cgacgcgccc gccgcgctgg cggccagcac cctgcttttc gaggccgtgc
     4441 ccgaaacgct ggacgccaag cgcgaggcgc tggaatacgc gggccggcat gccgcgccag
     4501 gcgccgtcat cgcctcgacc tcgtcgacca tgctggccgg cgagctggcc tcgctggtcc
     4561 cgcaccccga gcgctttctc aatgcgcatt ggctcaatcc cgcctacctg atccccctgg
     4621 tggaagtctc gccgcacgcc ggcaccgcgc ccggcgcgct cgaacagacg cttgcctcgc
     4681 tgaaggcggc cggcaaggtg ccggtggtgt gcaacgacaa gcccggctac atcgtgccgc
     4741 gcctgcagac cctggtcatg aacgaggcgg cccgcatgat cgaggaaggc accgccaccg
     4801 cggccgacat cgaccgcgcc atccgctacg gcttcggccc gcgctatgcg gccatgggcg
     4861 tggtggagtt catcgacttc ggcgggctgg acatcctcta tcacgccagc cgctatctca
     4921 gcacccgcct ggatccgtcg cgctatgcct gcccgcagat catcgaagac aagatgcagg
     4981 ccggcgaact gggcatgaag accgggcaag ggctctatgc ctgggatgcg cagcaggccc
     5041 ggcacttcca gcaggatgcg ctggcgcgcc tggtgcgcgt cctggccctc gacacgcaag
     5101 cgccggcgcc acggacgaag tagaccgctt tcctccgacg cgagcggcgc cgggctgttc
     5161 acggtacgta ccactggagt tcactcgcat ggctttcaaa ccgatgaagt atgttctgtc
     5221 cgccgtcctc gccggcctgt cctgcgcggc gctcgcgcag gacgtcccca cgctgcgcgt
     5281 gggctggacc atccccgccg aagaggccaa gtactggatg atgcgccgcc ccgaggcctt
     5341 ccccgacctg ggcaagacct acaagatcga atggacgcaa ttccagggca cggcgcccat
     5401 ggtgcaggcc atgatctcgg acgcgctgga ctgttcgtcg caggcgccgc tctcgctcgg
     5461 acaaggcgcc gtcaacggcg gcctgtcggc ctacatcgtg gcgcagcacg tcggcgaaag
     5521 cccctcgtcg ttctcggtgt actggggcgt gctggacggc tcgcccatca agcgcgtcga
     5581 agacctcaag ggcaagacgg tgggcgtgaa cgtgtacggc tcgggcgtgt acgggcagat
     5641 ggccgccatc ctgaagaagc atggactcga cccggcgaag gacgtcaagc tggtcgagac
     5701 cggctttccc ggctccgagt cggcgctgcg cggcggccgg gtcgacgcgg ccgtgttcgt
     5761 gcagcccttc gcggcgcgcg ccgaggccaa gggcggcatc cacaagctct tctccatgca
     5821 ggagctgatg cccaacaccg tgcagatcgt cgaggtctgc cgcaaggacg tcgccggcaa
     5881 gtcgcccgag ctgatcaaaa gctacgtgcg cgacctgacc cgcgccatga agatggccct
     5941 cgcgcaccgc gacgacacgg tcaagatcga ttccgaagtc acgcgcgcgc cggtcgaggc
     6001 gctggacacc tacctgctca agcccaacga ctttgcccac gaccccggcg cccaccccga
     6061 cttcgatgcc atccaggcca tgttcgacca gtacgccgcg ctgggcctgc tgagccgccc
     6121 gctcaaggtg tcggacttcc gccgggacga cctcgtcgcg ccgctgcaat aaggacggcc
     6181 ccatgtccaa gcgcctgatc gagatcgccg gactgtccaa gcgcttcgac gtgccgggcc
     6241 ggcccgcgca cctggcgctg gacggcatag acctgggcat cggagaaggc gagttcgtct
     6301 cgctgctcgg cccttccggc tgcggcaagt cgaccctgct ctacatggtg ggcggcttca
     6361 tcgagccgtc ggacggcgag atccgcgtgg acggccggcc cgtgcgcggg ccggggccgg
     6421 accgcggccc cgtgttccag gagttcgcgc tctttccctg gaagaccgtg ctgcagaacg
     6481 tcatgtacgg cctgagggtg cagaaggtgc ccgcgccgca ggccaggcag cgggccgagg
     6541 cgctgctgcg caaggtgggc ctggccgatt acgcgggctt ctatcccaag cagttgtcgg
     6601 gcggcatgcg ccagcgcgtc gccattgccc gcaccctggc ctacgagccg cgcatcctgc
     6661 tgatggacga acccttcggc gcgctggatg cgcacacccg caccacgctg cagaacgaac
     6721 tgctggccat ctgggagcgc gaccgcaaga cggtgctgtt cgtcacgcac ggcgtggacg
     6781 aggccgtcta cctgtcggac cgggtcgtgg tgatgagcgg ttcgccgggc agcgtgcacc
     6841 gtatcgtcga catcgacctg ccgcggccgc gcgtgcgcgc gcagctgctg accgacccgc
     6901 gctaccagca gtacgtgctg gaactcgagg ccatgctcga ccacgcccgg gtgccggagg
     6961 cggccgcatg aagcggcgga tgggctggcg cagggggtgg cgcgcgctgt tggcctgcgc
     7021 ggggctgctg ctggcctggc aggccgccgc gctatggctg aagatggaag aactgcccag
     7081 cgcctgggaa accctgcgcg ccgtccccgc catcctgtcc aacggcgagg acctgctcag
     7141 catcctggcc tcgctgcggc gcatggcgct gggtttcggc ctggggctgc tgatcgccgt
     7201 gccgctgggc ctggccatgg gccgcagcaa gccgctcgct cgcctgctca atcccttgac
     7261 ctccctggcc taccccatac ccaaggcagc cctgatgccc atcatcatgc tgtggttcgg
     7321 catcggcgac ctgtccaagg tgctggtgat cttcatgggc gtgagcctgc ccttgctcta
     7381 tcacagcagc cagggcgccg agcaggtgga cgacaagctg gtctggtcgg cccaggccat
     7441 gggcatgggc cggctggcac gcctggcgcg cgtgatcctg cccgccgccc tgcccgaggt
     7501 gatgctgggc tgccgcgtgg ccgtctcgat ggcgctgatc accatgatca gctcggaaat
     7561 gatcgcgcgg cgcaacggcg cgggcgacct gctcttcaat gccatggaca tggccgtcta
     7621 caccgatgtg tacgccgtca tgctcatcat cgccgccatc ggcgtggcgc tcgacgtgct
     7681 ggtggaacgc ctgcgcgcgg cgctcacgca ctgggccgac acactgcaac ccggatcggc
     7741 gggatgaaac gaccctcacg ctcatatggc gcggcctggc ccgtcatcgg cctgctgctg
     7801 gtctggcagt tggtctgcgc aagcggcctg gtgccgccgg cgctcctgcc cgcgccgctg
     7861 accatcgcgc tgcgcctggc gcaagagctg cttgaaccgg gcttcctttt tcacatcggc
     7921 cagacggcaa tacgcctgtt cgcgggcttt ttcattgcgc tggcgctggg actgggactg
     7981 ggcatcgcca tggcgcaggg cgccgaaccg ctgctcacgc cgctggtgcg cgtggccgcg
     8041 cccatcccca agatcgcgct gtacccggcc ctggtgctgc tgctgggttt cggccacgcc
     8101 tcgaaaatcg ccctggtggt ggccgacgcc gtgttcccgc tgatgctggc caccctgcac
     8161 ggcctgcgtt ccgtgcagcc caagctggtg tgggctgcgc aggccgcggg cgcgagccgc
     8221 tccgccatcg tgctgaccgt gatgctgcct tcggccctgg ggccgatact cgccggttgc
     8281 cgcatcgggc tggtgatcgc ctgcgtgaat gtattcctgg cggagatgat ctcgtcgagc
     8341 gacggactgg gccacctgct ggtgaccgcg gcgcgctcct accaggtggt ggacatgttc
     8401 gtgcccctgg tgctgatctc gctcatcggc ctggcgctga acgcctcgct acaattcgcc
     8461 cgacaccgct tcgccgccgc gctctaatcc atgcccgctt ccgcctatcc ggggcacgac
     8521 cagttcctgc cctatctcat caaccggact gccacgcaga tcaaccgccg gctgctggac
     8581 atcctcgacc agcacggcct gaccatgacg cattggcgcg tgctggcctt cctgtcccgg
     8641 caggacgacc tgagcatcgg cgagctggcg caggccacca tgaccgagca gtccaccctg
     8701 tcgcgctcat tgcgggcgct ggaagcgcgc ggctatgtgc ggcggcgcgc cagcagcacc
     8761 gacacccgcg ccgtgcacgt gcacctggag ccccagggcc gcaaggcctt cgagtccatg
     8821 ctggcgccgg ccctggagct ggaggccgtg tacatggaag gcatcagcga ccgcgaccgg
     8881 cagaccctgc gcaagctcct gcagcgcgtc ctgcagaact gcgaaggacg ggcagcttag
     8941 acggattcga atgatccgtt agaacatttg aaatagacgt tccaggccaa catttttaga
     9001 ctcctcgtct cattacgaca attatcgagg agacgatgtg ctgagccgag cgctggacaa
     9061 cgtccgggta ctggatctgt cgcgcatcct ggccggccca tggtgcaccc agaatctcgc
     9121 ggacctggga gccgacgtca tcaagataga gcgcccgggc caaggcgatg acacgcgcaa
     9181 atggggcccg ccctatctgc gctcggcaga tggcagcgac gccatggccg cgtacttcac
     9241 ctgctgcaac cggaacaagc gctccgtctg cctggacttt tcttcgcagc ctgaccgcaa
     9301 gctattgctc gatctcgtgc gctcttccga cgtcctggtg gaaaactaca aaacgggcac
     9361 gctcaaacgc tacggtttcg actacgacag cctcaaggcc atacgtccgg atttgatcta
     9421 cttatctatc accggctacg gccaaaacgg ccccaaggcc agccagccgg gctacgacta
     9481 catcttccag ggcataggcg gattgatggg ctataccggc cgggccgatg gagaagccgg
     9541 cgccggaccg ctgcgtgccg ggatccccgt cgtggacatt tccaccggca tgtacgccac
     9601 gtccgccgtg ctggccgccc tgctacaacg cgcccgcacg ggcaagggag cctatctgga
     9661 catcgccctg ctggatgtcg ccgtggcgat caacgccaac cagggcgcga actacctcgt
     9721 ctcggggaaa aaccccgagc gcagcgggaa tgcccatccc aacctggccc cctacgaagt
     9781 cttcaaggcc cgggacggct acttcatcct cgccattggc aacgacgggc aattcctcaa
     9841 gctttgcgaa ctttgcgggc atccggaact ggccgccgac ccacggttct cgaccaactc
     9901 cgcgcgcatc ggaaatctgc ccgccttgcg aggcatcctc ggcgacctca ttgccttgca
     9961 cgacaggtcc cactggacgt cgagcctgga agcggccggc atcgcatggg gccccgtcaa
    10021 tacgctcgac gaagttttca acgacgagca ggtccgccat cgcggcatgc tccggcatgc
    10081 gccacacccg cgtttcgggg aaatcccctc ggtccgcaat cccatgctga caggcgagga
    10141 tacagccccc atgcgacctc ccccattgat gggagaacac actcaagaag tcctagacag
    10201 cctttaattc aaaaaacagg agagagacat gcgattcaaa gcgatgaacg ccgcactgac
    10261 ggcgctggtt ttcggcacgg cctcggcttg cgcccttgcc ggttatcccg accacaacgt
    10321 gaccttggtc gttccctatg cgccgggcgg gaccaccgac ctcatggccc gcgcggtggc
    10381 gccaaaactg ggcgaggtgc tgggccaggc ggtggtcgtc atcaacaagc ccggcgctgg
    10441 cggcgaactg ggcagcgcct acgcggcccg cgaacgcccc gacggctaca ccctggtcat
    10501 ggacgtcgaa agctcccacg ccgtcattcc caacgtctac aagaaaccca gctacgatcc
    10561 cgtcaaggat ttttccccca tcagcaacct ggccaatgtc ttgggagtcc tggacgtgcg
    10621 ggccgattcc gagatcaaga cgttcggcca gctggtttca gtgctcaaag ccaatcccgg
    10681 caaatattcg ttcggctctt ccggcgcggg aggctatagc catttgttcg gcgagctgtt
    10741 cctgagcagc accggcaccc agatgctgca tgtcccctac aaaggcctcg gccccgccct
    10801 ggtcgacctg atgtcgggac aggtccaggt cgttttcgac aatcttccct cctcggcggg
    10861 ctttttgcag ggaggaaaac tgcgcgccct cgccgtcgcg tcgccggccc ggatcaagga
    10921 aatgcctgat gtcccgacct atgccgaggt cggctacccg cagctcaaca cgccctcgtg
    10981 gtttggcttg gccgcgccgg cgggaacgcc cgaacccatc ctcgatcagc tgaacctggc
    11041 cgtcaaaaaa gtattgaccg atcctcgcgt gatcgaaatc atccagaagg ccggcgccgt
    11101 tcccgactac acaacgcggg agcagttctc ggagatgatc aagcactcca acgctgtctg
    11161 gaaaaaagtc gtcgacgaca tccacttcga aaaactctga gccgccgccg catcatgaca
    11221 gcctcattcc atgcccccca tgccgatttc acgctgaaag ccaacggggt tggcatgctg
    11281 aacatctgcg acgcgggaaa gctcaatatc ctcggcacgc cggtcgtcaa cggcctgatc
    11341 gacgcattgg cccgcatcgg ccagcaaacc aggatcaaag tcctggttct aagggccgaa
    11401 caggaaaaag cgttcgtggc cggttccgac atcaaggaaa tggcgcattt cgatcgatct
    11461 tccgccatcg cttatatcga tcgactgcgc catttatgcg agctcgtccg gactttcccc
    11521 atgccggtca tcgcgcgcat cccgggctgg tgcctgggcg gcggcatgga gctggcgctg
    11581 tcctgcgatc tcaggatcgc ctcggatcaa gcccatatgg gcatgcccga ggtcaaggtc
    11641 ggagtgccgt ccatcatcca tgccgccctg cttccccgcc tcgtcggcaa ggcgcgcgcc
    11701 aactggatgc tgttgaccgg ggaaatcgtc gacgcgcccc aggcattgac ctggggactg
    11761 ctcgatcggg tagtatcagc tgacagcctg gatgcggagg tcgaccgcat cgccgacctg
    11821 ctggccggcc tgggagccgg cgtgctggcc cagcaaaagc gcctgctgag ggaatgggag
    11881 gacgaaccgc tgaacatctc catccagcat ggcgtgcttg aattcggggc ggcattcgac
    11941 acaggcgaac ccgggcgata tatgcgggcc tttatggaag gaaaagcccg caaggaataa
    12001 agccggatgc agcacgcgcc gcgtgccagg catgcttgaa cgaaaggaaa atccatgtct
    12061 tacgaatgga aagacttgca cctgttcaaa gcgatcatcc aactgggcaa tctttcggcg
    12121 gcggcggcgg ccatgcacat gacagctccg tcggccagct accggatcaa gaacctcgaa
    12181 tacacggcgg gcagccccct gttcgtccgg tccgccaagg gcatgcgatt gacggccgcg
    12241 ggcgaggccc tggctcgaca cgtggacatc ctcctggccg gcctgcatgc catgcaggcc
    12301 gagatcagcg cctatgcgca caatctcaag ggaaacatcc gtcttctcgc gaacagcagc
    12361 tctctcaacg gtttcatcat ccccagcctg gccagattcc tggcttccaa tctgcacatc
    12421 gacgtggatc tgaaagagca ggacagcccg tccatcgcgg gcaagatcct ccgtggagaa
    12481 gcggatatcg gcatttgctc cggaaaggtc gacgacccgg acctgcaatg cgaactctat
    12541 gccatagacc ggctcgtttg cgtcgtccac caggaacatg ccctggcaca acgagaccgt
    12601 tcacgttttt ccgaaatact ggatcacggc ctggtctgca tggctcgctc gagcagcaat
    12661 ttccaattcg tgctcgagca ggccgaacgg gcgggaaaac ccgccaatgt gcgggtgcat
    12721 gcccatgatt tcaactcggt tctttatctt gtgcgctcgg gcgtgggcgc ggcgctcgtt
    12781 ccggccagcg tcgcgcagcc cttcgtcagg gaaggcgctt tgaacagcat cacgctgctg
    12841 gacgcctggg cactgcgcga tctgcacttg atcacccgat ccgactcgga tccggccgga
    12901 ttggtgcggc aattcgcgga cgtcttgctg catgacccgc aagtcgccgc cgcccgctcg
    12961 gccggcgtca ccccgggatg gcacggggac atcggctgac cgcccgggct caacccacca
    13021 gcagcacgac cacggcggcc agcaggcagt agatgccgaa gaacgaccaa cggccctgct
    13081 ccagccactt cgacagccag cgcaaggcca ccaggccggc caggaagctg aacaccatgc
    13141 cgatcaggct ggggccgagc aggtgcgcgt agtccacggc ctgcgcgccg gccgtctgcg
    13201 cctgggcatg cgcgtcctgg tacaggcgcc aggcttcctt gacgatgacc ggcggggtca
    13261 gcaccacggc cagcgcgaag ctgaactcct cggcgcggcg gcggcccacg cccagggcca
    13321 ggccggtgga gatcgtcgcg cccgagcgcg agaagccgcg aaaaggcagg cacagcccct
    13381 gcacggcgcc gatccagaga ctgtcgcgca gcgtgagatc gccggaacgc tcgcggatgc
    13441 gcgaggccag gatgatgagc acgcccgccg cggccagcga tacggccatc agggtggcgt
    13501 tgccgaacag gtgctcgatc tcgaagctcg aggcgtggcg gtagaccagg tgcttgatga
    13561 tgaacagcag cgccaggccc accacgccgg tggcggcggt ggcgaccacc aggcgcagca
    13621 cattggcgcg gaaggccgag aacgacgaga aataggtggc gatccaggcc ttccagaagt
    13681 agacgatggc cgcgaacatg gtgccggtat gcagcatgac cagcagcatg gtcatggcgg
    13741 gcgcggtggg gtccagcccc atcagctttt cagccacgat cacgtgcgcc gaactggaga
    13801 cgggcagcag ctcggccgcg ccctgaacaa tggcaaggat caaaatctgt agcaaatgca
    13861 aatccatatc gaggcgcttc ctgttgatgg cccgaggtga ttgtgaatgg gcccggagca
    13921 gtttacgtca tgcttttgcc cgggccttgc atcctgcaac ggattagcgc gccctggggc
    13981 tatcccgcaa aagcagggac gagggcgcca ccccgaagcg cgccttgaag cggcgggcga
    14041 agtgggacag atcggaaaag cccgcggcga acgccgcctc ggacacgctg gccgccctgc
    14101 cttgcgtgag cgcgccatgc gccctggcga gcctctggtc gacgatgtat tgctcgaggc
    14161 tgatgcgcgg ctcgctgtac tgctggaaca ggcgatgcgc cgtgcgcagc gagatgcggg
    14221 tccgcgcggc ggcctcggcg ggggtgaacc cgatgacgtc cagattgcgg tcgatcgcgc
    14281 gcagcagctg ttcgtaggcc atgcggcccg gcaagttggc gcccctcgcg ccgcgcaggc
    14341 gcaaggccag caggtcggcg atattggcca gcagcacttg cacgtcggcg tcgccgacct
    14401 cggcgggccc ggtcagcacc tgcatggtgt gcgccatgac ggacgtgacg gcatcgtccg
    14461 cccccagccg caagggctcg tccagcatgc caaagcgcgg caggcgcgcg gccaggagcg
    14521 cgcggggcag catcacgatg cgccgctcga tggcgtccgg aaaatgcagc tggaacggct
    14581 gcgcgctgtc gatcacgccg atatctccgc cgcccaggct gaccccgccc ttgcgttgct
    14641 ccatatgcga cgcgccccgg cattgcaggc tgatcatcag gtactggtcg tcgccctggt
    14701 tgacctgccg ggcattgcgg atgatgcggt gcgcggagga acggaaattc acaaggcgcg
    14761 agccggcctg gtcgcggctc tggatgacgc cgtcgaaaag cagggggtcg ccgggctcgt
    14821 cgggcgtcat ggtcacatgc agcaccgcct ggcagacgac gtctttccag taatccaccc
    14881 gctgcaaggc ggaaccctcg gcctgccaat gccgccaggc cggccgcgag gacatggcgg
    14941 gactgctgcc gggcgaattc atgcgtactc ccccatacgt actcccataa accggccgcg
    15001 aacattggcg cggtaggaac aaaacagtgg cgcggtcggg ctagccgggc cggtagggga
    15061 ccgtcacact gggctcgaac gttttccggg ccgggcttgt tcattgaact gccgcccagg
    15121 caccggacat ataggggaat tcctgtgtcg cgcccggccc ccccgtttcc actaccgaca
    15181 gcctctggta cacgcaatga gccatcaacc cccatccgat gccctggccg ccttcgtcac
    15241 gcaggtgcgc gccgaactgg gcgaagacgc aatcacgctg gccgacgaaa ccctggcccg
    15301 ctacggcgag cacacgctgc ccgcgcccga ccagccgccc ggcgcggtcg cctacccggc
    15361 ttgcacccag gatgtgcaaa agctggtgct ggcggccaac cagcacaaga tcccgctctt
    15421 tcccatcagc gccggacaga acatcggcct gggctcgaaa tcgccggtga ggccagggca
    15481 ggtcatcgtc gacctgggcc gccacatgaa ccgggtcatc gaggtcaacg acacattggg
    15541 ctattgcgtg gtcgagcccg gggtgagttt ccaggcgatg catgatgaac tgcagcgccg
    15601 cggcagcaag ctgatgatgt cccccaccgc cggaccgccg cagggcagcc tgctgggcaa
    15661 cgcgctcgac aagggcggcg gcggcggtgc cgccggcgac cacttcggca tgtcgtgcgg
    15721 catggagatc gtactgggca acggcgagat catacgcacc ggcgacggca gcctcgactc
    15781 gcccgaacag ctgaactggc acgtctccaa atacagtttc ggccccgccc tggacggcct
    15841 gttcacgcag tccaactacg gcatcgtgac gcgcatgggc atgtggatga tgccgcgtcc
    15901 gccgcatatc gagccgttct tctttacctt ccccgacgat gacgacctgg gactgatcat
    15961 cgatttgatc cggccgctca agcagaccaa cttcgtgccc acgcagatcc gcgccaccaa
    16021 cgacctgtac ctgctctcgt ccgccgcctc ccaccccgaa tacgccgcca cgcggggcaa
    16081 ggaacagatc agcgacgcag cccgccgcga gctgcagcgt tcgttcggcc tgggcgcctg
    16141 gacggtatcg ggcgctttct acggcgccag cgcgcaggcg ctgcaaccgc agctggaacg
    16201 cgtgcgcgcc catttcacgc aaggcggcaa gggacgccat atccctcccg aggaggcggc
    16261 cgacatcgcg ccgctgcaca tcgcgcgcct gaactacgcg ggcgtgcccg gcgagggcga
    16321 actgcgcatg ctgcgctggc ggccgggcgg cgggacgagc tggttcctgc cgggcacgcc
    16381 catgctgggc gacgtcgcca atgccttcca gaaacgctcg cgcgccatct gcaaggaata
    16441 cggactcgaa tacatggtgt ccaacgtatg cgggccgcgc tttgcccgcg gcgtgcacac
    16501 cttggtctac aaccggcagg accccgacga gtccgcgcgc gccgacgcct gctatcgcgc
    16561 gctggcggcc tctttcgccg agcgcggcgt gttcgtgggc cgggcgccga cgctttacca
    16621 ggcgatgcac caggatcacc gcctgcccgc cttcaaccag gcctgcgccg ccatcaagcg
    16681 cgcgctcgac cccaacggca tcatcgcgcc cggcaaatac ggcatcgaat gaaccgcgtc
    16741 caaccaatct cttccatagg ggcaagaaaa ccatgaggaa atcgcttgcg gcaatcagcc
    16801 tggccatcgc gggcacgctg tcgatgttcc acgccgcctg ccgggcgcaa gactaccctg
    16861 cgcggcaagt caggctggag gtcggttttc cgcctggcgg gggcaacgac ctgttggcgc
    16921 ggctgctcgg cgcggagctc gaacgcgccc tgggccagcc cttcatcgtc gagaaccgcc
    16981 ccggcgcgca gggcttcatc gccttcaaca cggtcaaaac ggccaatccg gacggctaca
    17041 cgctgctgat cggcccgagc agcggcatga cggtcaaccc ggaggtctat cgcacgctgc
    17101 cctacgatgc catcaaggac ttcgccccga tcagcctcat cggcgccttc cccttgatcg
    17161 tcaccgcggc gcccaaggcg ccgttcggca cgctgaaggc attgatctcc gcggccaaga
    17221 gccagccggg caccatcaac tacgcctcgg cggccagctc attccagctc gccaccgaaa
    17281 tgttcgcgca acaggccggc atcaacctgc acaacatccc gtacaagggc agcgcgcaat
    17341 cggtccaggc cgtcatgggc aatcaagtac cgctgacctt tgccgacagc gcggccgtca
    17401 tcgcggagat caagagcggc gccctgcggg cgctggccgt gaccagcaag acgcgcgtgc
    17461 ccacgctgcc cgaggtgccc accgtcgccg aagccggcct gccgggctat gaaatggtgc
    17521 tgtggtccgg cctgttcgcg cccgccggaa cgccgcgcga catcgtcgac acgctgcagc
    17581 gcgccgtggc ccaggccatc catgccccgc agatgcagga aaagctgcaa ttgctcggcg
    17641 tcatgccggt gggcagcacg gcggacgaac tggccgacac catgcaacgc cagatctcgg
    17701 aatacacccg ggtagccaag acggccaaca tcacggtcga ctgagccgct cccgcaagct
    17761 catgccttca tcagcttgtc cgtggcgcgc cgcgcggctc cggtaaacaa ataaaaaaag
    17821 cgcggcgatg aaaaccgccg cgcccaaaac gacagccatc cgtcgccagg caagacgctg
    17881 aagctcagcg gaacacgctg accgcctcga ccagcctggt ggcctgctgg cgcaggcttt
    17941 ccgaggccgc cgtgctctgc tcgaccagcg cggcgttctg ctgcgtgatc tcgtcgaggc
    18001 tgcccacggc ctggtcgacc tgggtcacgc cggcgctctg ctgctcggtg gcggagctga
    18061 tctcggcgat caggtcggag acgcgctgca cctgcgtcac gatctccacc atggtcttgc
    18121 cggcgtcatt gaccagcgac gaacccgcgc cgatcttgtc ggcgctgtct tcgatgagct
    18181 gcttgatttc cttggcggcc tgggcgctgc gctgggccag cgtgcgcact tcgcccgcca
    18241 ccaccgcgaa gcccttgccc tgctcgccgg cgcgcgcggc ctccaccgcg gcgttcagcg
    18301 ccaggatgtt ggtctggaag gcgatgccct cgatcaccga aatgatgttg gtgatcttgc
    18361 gcgaactgtc ggtgatgtcg tccatcgtct tgatgacttc ggtaaccgcc gtgccgccct
    18421 tggtggccgc ctcgcgcgcc gtgcccgaca gcatattggc ctgctgcgcc gtctcggcat
    18481 tgctcttgac cgtggccgtc atctgggtca tcgaggccgc cgtctgttgc acgctggtgg
    18541 ccgccgcctc ggtgcgcgag ctcaggtcgt tattgccctg ggcgatctcg ttgctggcgg
    18601 tctgcacgtt gacgacctgc tcgctcacgt cgtcgatcag ccaacggaac atcaggccga
    18661 gctggccgat ggcgcgcagg gtcatgccga tctcgtcgac gcggttcatg tgctcgaccg
    18721 aggtgctggc gcccgaggcc acctgcaggg cctgctcgag cacgaactcg agcggcctgg
    18781 agatctggcg ctgcagccag aacagggcca gcagcgtcag gacggcgacc ccgccgccga
    18841 aggcggccag gttcgggccc gccaggccgg cctgccagcc gcccaggatg aacaagggga
    18901 tcagcggcag catggccgtg cgcaggcggc cgcgtaccga cagggtctgg aaaaccgagc
    18961 gccagcgcag caggccggta cggacgacca ggcccttgtg gaagcgcttg ctgccctgcc
    19021 tgccttcgcg aaaggttttg tacagcgcct cggcgccgtc gatctcggcg cgcgagggtt
    19081 tggtgcgcac cgacatgtag ccggtggtct ggccacggcg aaccaccggc acggcattgg
    19141 cgcgcaccca gtaatggtcg ccattcctgc ggcggttctt gaccagcgcc gtccagggtt
    19201 cgccgccctt gagggtgttc cacatgtcgg cgaacgcctc tttgggcatg tcggggtggc
    19261 gcaccatatt gtgcggctgg ccgttgatct cttggggcga gaagccgctg acctggatga
    19321 aggccgcgtt ggcgtaggcg atatggctcc gcgtgtcggt cgtcgacatc agcgtcgcat
    19381 gatcgggaaa ctcgaactcg cgctgcgtga ctggctcgtt gatgcgcatg gggtgactcc
    19441 gaaggctttt ggaccggcat tactgccagg acgatggaat cctatatggg aggcacctcg
    19501 aaataaaatg cattgaacgc gcttatttgc ccattcttct tctatttaga atttcatgtc
    19561 cacataccta attaaaaata attacttaaa tttatcaata aaatcatata gttaattaat
    19621 aaaaaataag tcctcttttc attgcgtgcc agaaattact gaaaaatcat caaatcaaac
    19681 aatccagcgc atttaacagc ctgtgccatt ttcgaaaaat ctctgcccgg ccatgccgcc
    19741 ctgcatctgc tcaaacccga ccagatccag gaaagcctgc cgctgtgcta cctgtctccc
    19801 tggccgcacc tggtggccta tgcctcgctg ggcgccatgg ccgccctgtt cgggcgcttc
    19861 gcatcgccgg cggatcgacg gcgcatcgtg ggcctgtgcg gcatctggct gaccttggcg
    19921 gtgctgtcca tgtccgcagc cagcttcctg ggcatcgatc ccaccctgct cctgctgctg
    19981 ctggcgctgt cgtgcggctt ctttttcttc gtcgtgtcga gctcgggctt cggcccgccg
    20041 ggcgccctga ttttcgtgtt tgccgtcggc gcctcgatga gccgggccga gtcctggcac
    20101 gccgtgttcg agcgcaccgc ggcaaccgcc gccgtcgttc ggcgcctgca tcgcctgggt
    20161 gatcctgaag caggcgccgt cggtctgggc cgtgtcgtgc tggcccgcgg cccgaggccg
    20221 cggcgcgtca gcggtattgc aggcgcatga agctggccat gctggccagc ccggcaaaga
    20281 ccgcccccag ccacaaggcg gccaccgcgc cgtgcgtggt cgacacgtgc aagcaggcgg
    20341 ccaccagggc cgcgccggtc gactggccca gcaggcgcac cgtgcccacc atgccgctgg
    20401 cgccgccgct gcgctcgggc ggcgccgaag tcatgatggc gcggatattg ggcgactgga
    20461 agaagccgaa gcccgcgccg cacaacatca tgcgccaggc gatgctcagc accgacgggt
    20521 gcgccggcat cagggccatc gacaccatgc ccaggcacag cagggccagg cccacgccgc
    20581 ccagcatccc tgacgaatag cggtcggaca gccggccggc gatgggcgcg gccagcgcca
    20641 cgaccaccga ccagggcgtg atcaggaacc cggtctcgat ctgcgtgtag cccagcacgt
    20701 gctggaacat gaaaggcaag gagacgaagg ccagcgcctg cgtggcgaac gagcacacgc
    20761 cggtaagcgc cgacagcgcg aacatcggcc ggcgcagcag gtccacggcc agcaccggcg
    20821 ccgtcatgcc cgcctgccgg cgcagcagcc accacaggca cagcagcgtc agcacggcct
    20881 cgccgcctac ccgcccccag ccctgcccat gggcggcctc gttgagcgtg aagatcagca
    20941 gcgcgaaaaa caccgaacac aacaagccgg caagatggtc gaagcgctgc tggctacgcg
    21001 gcgtggccgg cagcgcgcgc aggcccaggt agatggccag caggcccaag ggcacgttga
    21061 ccaggaacag ccagtgccag ctggcaaaag agagaatgag cgaggcgatc gtcggccccg
    21121 cggcgaacga aacgccgacc accagcgcgt tgatgcccgc gccgcgcccc atctgattcg
    21181 atggatagat gaagcgcacc agggcgccgt tggccgacat cagcgcagac ccgcccaggc
    21241 cctgcagcac gcgggcgatc accagccatt ccagcgagga cgacagcccg cacagcagcg
    21301 aggccagcgt gaagagaacc aggcccgtca tcatgacgcg gcggtagccc acgatctcgc
    21361 ccagtccggc gaagggcagc aggcctgccg ccatcgtgag ctggtaggcg ctcacgatcc
    21421 agaccgacag cgattcggtc gtgcccaggt cgtgcgccat ggtgggcagc gccgtattgg
    21481 cgatggccgt atccaggctg gacatgctca cgcccaacac caccgacaac accgcgctca
    21541 agcgttcagc atcgggaacc ccttgtcccg agggatagac ctgggtgcgt gcaaagctaa
    21601 gtaacatgac gaagagttga atcagagaaa ggttcgcggc ctagtgaccg aaaagtgctc
    21661 gggcatatta ctctttcatc tgagatttct ccggagtaac cctcatgaaa ctggtacgtt
    21721 atggctcgcc cggccaggaa aagcccggcc tgatcgacgc gcagggcgcc ctgcgcgacc
    21781 tgtcgggcca cctcgccgac atcgacggcg ccgccctgtc gcccaccgtg ctggccgcgc
    21841 tggccggcat cgcccccgac agcctgcccc gtgtcgacgg cgcggtgcgc tatggttgcc
    21901 cggtggccgg catcggcaag atcgtctgca tcggcctgaa ctatgccgac catgccgccg
    21961 aagccggcct ggccgtgccc accgaacccg tggtttttct caagcccagc tcgtcgctca
    22021 gcggccccaa cgacccggtg acgatcccgc gcggttcggt caagaccgac tgggaggtgg
    22081 aactgggcgt ggtgatcggc cgcaaggcct cgtatgtcga ggaaagcgag gcgctcggct
    22141 acgtggccgg ctacaccctc gtcaacgacg tgtccgaacg cgaataccag atcgagcgcg
    22201 gcggccaatg ggacaagggc aagggctgcg acaccttctg cccggtcggc ccctggctgg
    22261 tgacggccga tgccgtgccc gatccgcagg ccctgccgct ctggctggaa gtcaacggca
    22321 agcgttacca ggacggcaac acccgcacca tggtgttcgg cgtgaagacg ctggtgagct
    22381 acgtgagccg cttcatgtcg ctcatgccgg gcgacatcat cagcaccggc acgccgcccg
    22441 gcgtcggcat ggggcagaag ccgccggtct tcctcaaggc gggcgacgtc atgcgcctgg
    22501 gcatcgacgg cctgggcgaa cagcggcagg ccctggtgga ctggaaacgc tgaaaccggc
    22561 cgcagaagca aaccacgcag aatcgaccca tgaagcgcag agcattcctc gtacacacac
    22621 tggccggcgg cgctctactg ctggcccgcc cggcctcggc ccagcctgcc ggccacgcca
    22681 tgcacggcat gtccggcatg caggggatgg agggcatgga aggcatggca cctgcccccg
    22741 ccacggccgc caaactggcc gccgacggcg ccctgcccgc cggcgcgccg ctggccgccc
    22801 tgccggcgct ggccaacgaa agcaggcagc ccggcctgtt tcgcgccacg ctggccgcgc
    22861 agccggcgcg ccaggccctc atgcaaggca aggccaccct catgtggcaa tacgcggccc
    22921 gcgccggcgg ccggccggcc ggcatcgcgg gcccgctgat cgacgcgcgc gaaggcgaca
    22981 ccctcgagat ccgcttcgag aaccatctgc cgcagccctc gaccatccat tggcacggcc
    23041 tgcccgtgcc gcccgagcag gacggcaacc cggccgacgc cgtggcgccc ggcgccgcgc
    23101 gcacctaccg ctacaccctg ccgccaggca gcgccggcac ctactggtat cacccgcatc
    23161 cgcacatgcg gacggcggaa caggtgttcc gcggactggc cggccccatc atcgtgcgcg
    23221 ccgccgacga cccgctggcc ggctggcccg aacggcattt gtttatctcc gacctgcggc
    23281 tggccgccga tggcagcatc ccggccaacg acatgaacga ctggataaac ggccgcgaag
    23341 ggcagttcgt gctgatcaac ggcgccctgc gccccgagat cgccatcaac ggcgacgagc
    23401 gctggcgcgt ctggaacgcc tgcagcgccc gctacctgaa gctggccttc agcgacggcc
    23461 gcgccttcac gcaggtgggc accgacggcg gcctgctgga acgccccgtc gaagggctca
    23521 aggaactgct gctggcgccg ggcgaacggg ccgagctcat cgtgcgcgcg ggcggccagg
    23581 cctcgcaggc cgcgctgatg gccctggcct acgaccgcgg caagatgaac atggtgggcg
    23641 acctgccgcc cgagaccggc cgcacgctgg cccaggtaac gttcgcgccc gcggcgggcg
    23701 acacggcgcg cccgctgccc gccacgctgc gcgccatcgc gccgctcggc gcacccgccg
    23761 cgaaaaagac cgtcatgctc accgagcaga tggacatgca gcaggacatg cacgccacga
    23821 acctgcaggg catgccggac ggcatgcgtt tcctgatcaa cggcgcgagc ttcgacccgc
    23881 agcgcgtgaa cctgaccagc cggcgcgacc aggtggaagt ctggaccttg cagaacttca
    23941 ccgacatgga ccaccccttc cacatccacg gcacgcagtt ccaggtgctc gaccgcaggc
    24001 acgagggcgc cacgacggcc gaaccctgcc tggcctggcg cgacacggtg aacctgcgtc
    24061 ccagcgaaac cgtgcgcatc gccaccgtgc agcgctcggc gggacaacgc atgttccact
    24121 gccacatcct cgagcacgag gacctgggca tgatgggact attgcaggtg gtctgatggc
    24181 cgggtgacgc ccggcgcgtc gtcacctggg atcgatgggc gtcgcgaaat gctgcgccgg
    24241 ctcgatggcc tgggccgcgc ggatcagcac gtcttcgcga aaccgcccgg ccaccagctg
    24301 gacgccgacc ggcacgccat cggcctcgcc ggccggcagc gacaaggccg gcaaccccag
    24361 cagcgcgacg gccagcagcg ggtgctggtc ctgcaggaag cgatgcatct gcgccgcgcc
    24421 gccctggtcg tggtcgacgg caaccggccg ccggaacgac agcggcagca gcagcagagg
    24481 atggtcgcgc agcagcaggt tccactcgcg ggccaaggca tggcgccggc ccaggccctg
    24541 gatatagcct tcgaaatcca gcgcgggcac gctgccgcgc gcattgcgcc gggccgtgcg
    24601 caccgcctcg tcgccgtggg cttcgatggc cttgagccgg tccagcgcct cttcggccga
    24661 ggccagttgc cggaacagcc gggccgcctc cgcgaaaccg ggcagctcga ggtcctcgac
    24721 ctcgtagccg gcgtcctgca aggcggcgcg cgtcctggcg atcgcctgcg cgaccgccgg
    24781 atcggtttca tagccgtcga cctgcgccgc gacgccgacc ttcaagcgcg gcgcctcctg
    24841 cgcaacgagc ggcgccggca cgtgccaggg atcgcgcggg tccggctgcg ccagcgccgc
    24901 caggcccagg ggcaggtcgg cggcccggcg cgcgaacagg ccggccacgc tggtgatctg
    24961 cgacgtgagt ccgggcgagg actgcgccgt ggcgttgtac aggggcaccc ggccgaagga
    25021 cggccgaagg ccatacaccc cgttgacgta ggcgggatag cggatcgaac cggagcggtc
    25081 cgtgccgtgg gccagggcgc cgatgcccac cgccgtggcc accgccgcgc cgccgctgga
    25141 cccgccgggc gtcaccgccc ggttccacgg gttgtaggtg cgcccgtgca ggtcgttgtc
    25201 ggtgaaatag cgcgcggtga aggcgggaac gttggtgcgg ccgatgatga ccgcccccgc
    25261 ccgccgcaga ttggcgatgg cgggcgcgtc ctccggcgcg atctggtcgc ggtaggccac
    25321 gaccccgcgg gtggtcggcc ggccggcgta gtcggtatcg atcttgaccg tgacgggcac
    25381 gccgtgcagg gcgcctatgg gctggccgtg ccggacggcc gcgtcggcgc gatcggcggc
    25441 ctgccgcgcg tcgtcgtaga gcacgtcgac caccgcgttg acatgcgggt tgaccgcgtc
    25501 caggcgcgcg aaggcgctgt cgacggcctg ccggctggag atttcacggc gcaggatggc
    25561 ttcggccagc tcggccgccc cccactgcca caagggtcgc gtcgtcatgg atgactcgtg
    25621 ctcacttccg ttggcgagct gcccaggata ggggccggtg cgggcacggg aaaatacttt
    25681 tcccgaataa ccttagttgc tcaacgcccg cccatcagcg cgcggccgga gtcggcgggc
    25741 agccggtagc acaggggcca cgccagcgcc gtgaccagcg cgacggccgt gaaggccagg
    25801 atgaaatcgc cacgcaccgg cgcctgccgc tgcgaccact gcatcgacaa gtgcagcacg
    25861 cacaccgaga aggccacgcc cagcgtcgcc gacaactgct ggaaggtgct gttcaggctg
    25921 gtggccgcgc tcatgcgggc ggacggcacg tcggcatagg cgaaggtgcc gtacacgttg
    25981 tactgcaggg agcgcgccat gccgcccagc agcagcaccg cggcgatcgc ggggatgggc
    26041 cagccgggcc ggaagacggc gcacagggcc agggagaccg tgcacgccac cccgttccac
    26101 aacagcacat tgcgatagcc gaatttgcgc aggaccccga cgctggcccc cttggcgccc
    26161 agcgcggcga tgccgctggc cagcatggcc agcccgctct gcatcgcgtt cagccccatg
    26221 cccaattgca gcatgagggg cagcaggaag ggcaaggcgc cgaacccgat gcgggccagc
    26281 gtgccgccga cgagcgcgac gctgaagctc tggatgcgca ccagggaaaa gtccagcacc
    26341 gggtgcgcgc agcgccgggc gtgcacgccg tacgccacgc cgctcacggc agccaccgcc
    26401 aggaaggcgc cgacccgggt ccagtccgcg ctgtcgcggc tgaagagatc cagcccgtac
    26461 atcgtggccg cgagcgcgac ccccatgagc agcagcccgc gcacgtccag cctggccggc
    26521 ttgtccatgc gcagcggcgg cacgtggatg agcgccatca ccaggccgag caggccgatg
    26581 ggaacgttga tgaagaagat ccagtgccag gaataatagg tggtgatgaa cccgcccagc
    26641 ggcggcccca gcatcggccc gatcatggcc ggcatcagca cccacgccat ggcctcgatc
    26701 aactgcgact tgctgaccga gcgcagcatg atgaggcggc ccaccggcac catcatggcc
    26761 ccgctg
//
LOCUS       DASWPO010000342         9923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2851062, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000342 DASWPO010000000
VERSION     DASWPO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2851062"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /gene="nuoK"
                     /locus_tag="VGC69_18220"
     CDS             complement(<1..218)
                     /gene="nuoK"
                     /locus_tag="VGC69_18220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY0297269.1"
                     /translation="MTLTLAHYLILGAIMFAIGIFGIFLNRRNLIILLMSIELMLLAV
                     NMNFVAFSSWAGDTAGQVFVFFILTVAAA"
     gene            complement(215..874)
                     /locus_tag="VGC69_18225"
     CDS             complement(215..874)
                     /locus_tag="VGC69_18225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0297270.1"
                     /translation="MTFITFLFYLLSLVLVIAAFRVITAKSPVTAVLHLILAFANAAM
                     IWMLLGAEFLALLLVLVYVGAVMVLFLFIVMMLDIRVDHLRRGMKTYLPLGLIVGGIM
                     VLEMAFVLGSTWGGNASPAAMAGNYNNARVLGQAMYSHYIYGVEVGAALLLVGMVSAI
                     ALTLRGRRSDTKHIDPAAAIRVRAQDRFRLVKMQAVSERAGNGDAGAADSAASAQGEK
                     Q"
     gene            complement(891..1379)
                     /gene="nuoI"
                     /locus_tag="VGC69_18230"
     CDS             complement(891..1379)
                     /gene="nuoI"
                     /locus_tag="VGC69_18230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY0297271.1"
                     /translation="MEAIKDFFGSLMLAELFKGLKLTGKYFFKRKITLRYPMEKTPMS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEVREDGSRRTTRYDIDLTKC
                     IFCGFCEESCPVDSIVETHIHEYHGEKRGDLYFTKDMLLAVGDRYEAEIAKRRAEDAP
                     YR"
     gene            complement(1396..2469)
                     /gene="nuoH"
                     /locus_tag="VGC69_18235"
     CDS             complement(1396..2469)
                     /gene="nuoH"
                     /locus_tag="VGC69_18235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY0297272.1"
                     /translation="MTWLDALESNGAALLGPTAWLVIWTIIKILVIAIPVILCVAYLT
                     LWERKMIGFMHARLGPTRVGPRGLLQPFADVFKLITKEIVLPTKSNKVLFIVSPVVTL
                     MPALAAWAVVPFQPEVVLANVNAGLLYIMAITSVGVYGVIVAGWASNSKYAFLGALRA
                     SAQMVSYELAISFVLVTVLLVSGSLNMSEIVQGQGRGWFASHGLTFLSWNWLPLLPLF
                     VIYVISIVAETNRHPFDVVEGESEIVAGHMVEYSGTGFALFFLGEYANMILLSCMASI
                     MFLGGWMAPFDIAPFTWIPGWIWLGIKTFLVVSMFIWFRAAFPRYRYDQIMRLGWKIF
                     IPLTGVWLVVVAIWMQTPWNIWH"
     gene            complement(2469..4793)
                     /gene="nuoG"
                     /locus_tag="VGC69_18240"
     CDS             complement(2469..4793)
                     /gene="nuoG"
                     /locus_tag="VGC69_18240"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY0297273.1"
                     /translation="MVELTVDGNKVEVPEGSMVMHAAQKIGLYVPHFCYHKKLSIAAN
                     CRMCLVEVEKAPKALPACATPVTNGMVVHTVSEKAKDAQKSVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGSASRYKEEKRVVFHKDLGPLVSAEEMTRCIHCTRCVRFGQEI
                     AGVIELGMVGRGEHSEITTFVGRSVESELSGNMIDICPVGALTSKPFRYSARTWELAR
                     RRSVSPHDSLGANLVVQVKDEEVLRVVPFENDAINECWISDRDRFSYEGLNSADRLAT
                     PMIKNVDGSWREASWADALQTVAQGLTRVRDSAGAGQIGALATEYATTEEFSLLGRLV
                     RALGSENIDFRLRQTDAGFDAALSGAPWLGMPITELDKLDRVLVVGSFLRKDHPLVAQ
                     RLRQAAKRGTQVLLIDGSADNQLMPVASRITTAPSGLATALAEVAVALAQAKSLPAPA
                     GFASVVPGEEAKRVAASLASGSNTAVLLGNLAVSSPLAATLAANASALAGLAGAKFGF
                     LTAGGNTVGGYLAGAVPGQGGKTAAAMLAEPLKAYVVLHAEPLLDADNGPQALAALQG
                     AFSVALTSYRSAAQDWADVMLPVAPFTETSGTFVNAEGTAQSFKGTVAPFGETRPGWK
                     VLRVLGNVLQLPSFDDESSESVRDAVLAGGIEGRLSNQIKAAPGASVVSSGLERLADV
                     PIYRSDAIVRRSGPLQATPASRPPVARMNGATLSQLGLASGAQVRVKSATGSVVLEAV
                     QDDAVAVRAVRIAAAFEQTAPLGGAFGEISVEHA"
     gene            complement(4809..6182)
                     /gene="nuoF"
                     /locus_tag="VGC69_18245"
     CDS             complement(4809..6182)
                     /gene="nuoF"
                     /locus_tag="VGC69_18245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY0297274.1"
                     /translation="MSALEQSLYQSLSQGLDPDPFNDLANSMALHGRHIKPQIMADVD
                     GANWRLADYVKRGGYEALRKILTTGMKPEDVIAEVKASGLRGRGGAGFPTGLKWSFMP
                     RTFPGQKYLVCNSDEGEPGTFKDRDILRFNPHIVIEGMAIAAYAMGITVGYNYIHGEI
                     FEVYERFEEALDEARAAGFLGDKILGSDFNFQLHAFHGYGAYICGEETALLESLEGKK
                     GQPRFKPPFPASFGLYGKPTTINNTETFAAVPWIIRNGGQAYLEVGKPNNGGTKLFSI
                     TGDVERPGNYEIPMGTPFSTLLELAGGMRGGKKLKAVIPGGSSAPVLPADIMMDTTLD
                     YDAIAKAGSMLGSGAVIVMDETRCMVKSLMRLSYFYFEESCGQCTPCREGTGWLYRMV
                     HRIENGQGRPEDLDLLDNVAGNIMGRTICALGDAAAMPVRGFLKHFRDEFAHHIEHKS
                     CVVPHYL"
     gene            complement(6179..6670)
                     /gene="nuoE"
                     /locus_tag="VGC69_18250"
     CDS             complement(6179..6670)
                     /gene="nuoE"
                     /locus_tag="VGC69_18250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY0297275.1"
                     /translation="MLLSAQAYQKIDRELAKFPADQRQSAIMAALAIAQDEKGWLSPD
                     ILEDVAKYIGVEPIAVEEVSTFYNMFNVAPVGKHKLSVCTNLPCALRSGERAGDYLKR
                     KLGIDYSETTADGQFTLVEGECMGACGDSPVLLVNNKHMCVRMTEEKLDALVDMLKKG
                     EAA"
     gene            complement(6718..7974)
                     /locus_tag="VGC69_18255"
     CDS             complement(6718..7974)
                     /locus_tag="VGC69_18255"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY0297276.1"
                     /translation="MAEIKNYTLNFGPQHPSAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAEHKTYLQALPYMDRLDYVSMMCNEHAYVMAIEKLLGVEVPLRAQYIRVMFD
                     EITRILNHLMSVGSHALDVGAMAVFLYAFREREDLMDCYEAVSGARMHAAYYRPGGVY
                     RDLPDTMAQFQGSTKYRSEKDIHIVNQARSGSLLDFIEDFTNRFPACVDEYETLLTDN
                     RIWKQRLVGVGVVDPDRAKALGFTGPMLRGSGIAWDTRKTQPYEVYDLLDFDIPVGVN
                     GDCYDRYLVRVAEMRQSNHIVRQCVEWLRNNPGPVMSDNHKVAPPHRVGMKTNMEELI
                     HHFKLFTEGFHVPPGEVYSTVEHPKGEFGIYLVSDGANKPYRLKIRAPGFAHLQSLDE
                     MTRGHMIADAVTIIGTQDIVFGEVDR"
     gene            complement(7987..8625)
                     /locus_tag="VGC69_18260"
     CDS             complement(7987..8625)
                     /locus_tag="VGC69_18260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY0297277.1"
                     /translation="MTTRLETLKTHLQAVFGQEAAITEAFGELTLEVPAEQWFSACTK
                     LRTDAGLRFESCLDLCGVDYLTYGNATRIEREEQGAGIQRGPHGGRYAVVVHLLSFEH
                     NWRLRVRTWAPDDEFPMVASLVECWPSVNWFEREAFDLYGIVFEGHPDLRRILTDYGF
                     IGHPFRKDFPLSGTVEMRYDSEQHRVVYQPVTIDPREITPRVVREDTYGMGR"
     gene            complement(8633..9109)
                     /locus_tag="VGC69_18265"
     CDS             complement(8633..9109)
                     /locus_tag="VGC69_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY0297278.1"
                     /translation="MAIDGILKQGFITTSADKFINWAKTGSMWPMTFGLACCAVEMMH
                     AGAARYDLDQFGIIFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMPEPRWVVSMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGLLQMQNKIRLTNTIAR"
     gene            complement(9188..9547)
                     /locus_tag="VGC69_18270"
     CDS             complement(9188..9547)
                     /locus_tag="VGC69_18270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY0297279.1"
                     /translation="MNLQQYFPVLLFILVATLIGFALITAGSLLGPKRPYAEKLSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAIAQGTLGLAGFWTVMVFLAV
                     LTVGFIYEWKKGALDWE"
BASE COUNT         1810 a         3027 c         3312 g         1774 t
ORIGIN      
        1 gcggcggcca cggtcaggat gaagaacacg aacacctggc cggcggtgtc gccggcccag
       61 ctcgagaacg ccacgaagtt catgttgacg gccagcagca tgagctcgat ggacatcagc
      121 agaatgatca ggttgcggcg gttgagaaaa atgccaaaga taccgatggc gaacatgatc
      181 gcccccagga tcaggtaatg ggccagcgtc agcgtcattg cttttctcct tgtgccgagg
      241 cggcggaatc ggccgcgccc gcatcgccgt ttccggcacg ctcgcttacc gcctgcatct
      301 tgaccaggcg gaagcggtcc tgggcgcgaa cgcgaatggc cgcggcggga tcgatgtgct
      361 tggtgtccga gcggcggccg cgcagcgtca gcgcgatggc ggaaaccatg cccaccagca
      421 gcagcgcggc accgacttcg acgccgtaga tgtagtggct atacatggcc tgccccagca
      481 cgcgggcatt gttgtagttg cccgccatgg cggccggcga cgcgttgccg ccccaggtgg
      541 agcccagcac gaaggccatt tccagcacca tgatgccgcc cacgatcagg cccagcggca
      601 ggtaggtctt catcccccgg cgcaggtggt cgacacggat gtcgagcatc atcacgatga
      661 agaggaagag caccatcact gcgcccacgt acaccagcac cagcagcaga gccaggaact
      721 cggcgcccag cagcatccag atcatggccg cgttggcgaa ggccaggatg agatgcagca
      781 cggcggtcac ggggctcttg gccgtgatga cccggaacgc cgcgatcacc aggacgaggg
      841 acagcaggta aaacagaaaa gtgatgaaag tcatgttcta accctggcga tcagcgataa
      901 ggcgcatctt cggcccgacg cttggcgatt tcggcttcgt agcggtcgcc cacggcgagc
      961 agcatgtcct tggtgaagta caggtcgccg cgcttttcac cgtggtattc gtggatgtgc
     1021 gtctccacga tggaatccac ggggcagctt tcttcgcaga agccgcagaa gatgcacttg
     1081 gtcaggtcga tgtcgtagcg cgtggtgcgg cggctgccgt cctcgcgcac ctcggactcg
     1141 atggtgatgg ccagggccgg acacaccgcc tcgcacagct tgcaggcgat gcagcgctct
     1201 tccccgttgg ggtagcggcg cagcgcatgc aggccgcgga aacgcggcga catgggcgtc
     1261 ttctccatgg ggtagcgcag ggtgatcttg cgcttgaaga aatacttgcc cgtcagcttc
     1321 aggcccttga agagctctgc cagcatgaga ctgccgaaaa aatccttgat cgcttccata
     1381 tcttgcctcg gccgctcagt gccaaatatt ccagggcgtc tgcatccaga tcgccaccac
     1441 caccagccac acgccggtca gcgggatgaa gatcttccag cccagacgca tgatctggtc
     1501 gtagcggtaa cgcgggaagg ccgcgcggaa ccatatgaac atcgacacca cgaggaaagt
     1561 cttgatgccc agccagatcc agcccgggat ccaggtgaac ggcgcgatgt cgaacggcgc
     1621 catccagccg ccgaggaaca tgatcgacgc catgcatgac agcaggatca tgttggcgta
     1681 ttcgcccagg aagaacagcg cgaagcccgt gcccgagtac tcgaccatgt ggccggccac
     1741 gatttccgac tcgccttcga ccacgtcgaa ggggtggcgg ttggtttccg ccacgatcga
     1801 gatcacgtag atcacgaaca gcggcaaaag cggcagccag ttccaggaga gaaaagtcag
     1861 gccgtgcgac gcgaaccagc ccctgccctg cccttgcacg atctcggaca tgttgaggct
     1921 gcccgagacc agcagcaccg tcaccagcac gaagctgatg gccagttcgt acgagaccat
     1981 ctgcgccgag gcgcgcagcg cgcccaggaa ggcatacttg gagttcgacg cccagcccgc
     2041 cacgatcacg ccgtacacgc ccaccgaggt gatggccatg atgtagagca ggccggcatt
     2101 gacgttggcc agcacgactt caggctggaa aggaaccacc gcccaggccg ccagggccgg
     2161 catgagcgtg accaccggcg acacgatgaa caggaccttg ttggacttcg tgggcaggac
     2221 gatttctttg gtgatcagct tgaacacgtc cgcgaagggc tggagcaggc ccctcggacc
     2281 gacgcgcgtc ggtccgaggc gagcgtgcat gaagccgatc atcttgcgtt cccacagcgt
     2341 gaggtaagcc acgcacagga tgacgggaat tgcgatcacg agaatcttga tgatcgtcca
     2401 gatcaccagc caggcggtgg ggcccaacag ggccgcgcca ttgctttcaa gtgcatcaag
     2461 ccaagtcatc aggcatgctc cacgctgatt tcaccgaagg caccgcccag gggagcggtc
     2521 tgctcgaagg ccgctgcgat gcgcacggcg cgaaccgcca ccgcatcgtc ctggacagct
     2581 tccaggacca ccgaaccggt ggccgacttg acgcgcactt gcgcgccgga cgccaggccc
     2641 agctgcgaca gcgtggcgcc gttcatgcgc gccacgggcg ggcgcgaggc cggcgtggcc
     2701 tgcagcggtc cggaacggcg cacgatggcg tcggatcggt agatcggcac gtcggccagg
     2761 cgttccaggc cggaggaaac cacactcgcg ccaggcgcgg ccttgatctg gttggacagg
     2821 cggccctcga tgccgccggc cagcacggca tcgcgcaccg actcggagct ttcatcgtcg
     2881 aagctcggca gctgcagcac attgcccagc acgcgcagca ccttccagcc cggacgggtt
     2941 tcaccgaacg gcgcgaccgt gcccttgaag ctttgcgcgg tgccttcggc gttgacgaag
     3001 gtgccggagg tttcggtgaa cggtgccacc ggcagcatca cgtcggccca gtcttgcgcg
     3061 gccgaacggt aggacgtcag cgccacggag aatgcgccct gcaacgcggc cagcgcctgc
     3121 gggccgttgt cggcgtccag cagcggctcg gcatgcagca ccacgtaggc cttgagcggc
     3181 tcggccagca tggccgccgc cgtcttgccg ccctgccccg gcacggcgcc tgccaggtaa
     3241 ccgcccacgg tgttgccgcc ggcggtcagg aagccgaact tggccccggc caggccggcc
     3301 agcgcgctgg cgttggcggc cagcgtggcg gccaacggcg aactcacggc caggttgccc
     3361 agcagcacgg ccgtgttcga gcccgatgcc aggctggcgg cgacgcgctt ggcctcttcg
     3421 cccggaacca ccgaggcgaa cccggccggc gccggcagcg acttggcctg ggccagcgcc
     3481 acggccactt cggccagggc ggttgccagg cccgagggcg ccgtggtgat acgcgaggcc
     3541 acgggcatca gttggttgtc ggccgagccg tcgatcagca gcacttgcgt gccgcgcttg
     3601 gcggcctggc gcaggcgttg cgcgaccagc gggtgatcct tgcgcaggaa cgagcccacg
     3661 accaacacgc ggtcgagctt gtcgagttcg gtgatgggca tgcccagcca gggcgcgccg
     3721 gacaacgcgg catcgaagcc ggcgtcggtc tggcgcaggc ggaaatcgat gttctcggaa
     3781 cccagggcgc gcaccaggcg gcccagcagg gagaattctt cggtggtggc gtattcggtg
     3841 gccagggcgc cgatctggcc ggcgccggcg ctgtcgcgca cgcgggtcag gccttgcgcc
     3901 acggtttgca gggcatcggc ccaggaggct tcacgccagg agccgtccac gttcttgatc
     3961 atgggcgtgg ccagacggtc ggcgctgttc aagccttcgt acgagaaacg gtcgcggtcg
     4021 ctgatccagc attcgttgat ggcgtcgttc tcgaagggca ccacgcgcag gacttcctcg
     4081 tccttgacct ggacgaccag gttggcgccc aggctgtcat gcgggctcac cgaacggcgg
     4141 cgggccagtt cccaagtacg ggcgctgtag cggaaaggct tggaggtcag cgcgcccacc
     4201 gggcagatgt cgatcatgtt gcccgacagc tcggactcga cggagcgccc cacgaaggtg
     4261 gtgatttcgg agtgctcgcc gcggcccacc atgcccagct caatcacgcc ggcgatttcc
     4321 tggccgaagc gcacgcagcg ggtgcagtgg atgcagcgcg tcatttcctc ggccgacacc
     4381 agcgggccca ggtccttgtg gaagaccacg cgcttttctt ccttgtagcg cgaagccgag
     4441 ccgccatagc ccaccgccag gtcttgcagc tggcattcgc cgccctggtc gcagatgggg
     4501 caatcgagcg ggtggttgat gagcaggaat tccatgaccg acttctgcgc gtccttggct
     4561 ttctcggaca cggtatgcac caccatgccg ttggtcacgg gcgtcgcgca ggccggcagg
     4621 gccttgggcg ccttctcgac ctcgaccagg cacatgcggc agttggccgc gatggacagt
     4681 ttcttgtggt agcagaaatg cggcacgtag agcccgatct tctgggcggc atgcatgacc
     4741 atgctgccct cgggcacttc gaccttgttg ccgtcgacgg ttagttcaac cattgctgtt
     4801 cctgaggcct acagatagtg cggaaccaca cacgatttgt gctcgatgtg gtgcgcgaat
     4861 tcgtcgcgga agtgcttgag gaaaccgcgc acaggcatgg cggcggcatc gcccagcgcg
     4921 cagatggtgc gacccatgat gttgccggcc acgttgtcga gcaggtccag gtcttccgga
     4981 cggccctggc cgttttcgat gcgatgcacc atgcggtaga gccagccggt gccttcacgg
     5041 cacggcgtgc actggccgca gctttcttcg aaatagaagt acgacagccg catgagcgac
     5101 ttgaccatgc agcgggtttc atccatgacg atgaccgcgc ccgaacccag catggaaccc
     5161 gccttggcga tggcgtcgta gtccagggtg gtgtccatca tgatgtcggc gggcagcacc
     5221 ggggccgacg agccgccggg gatgacggcc ttgagcttct tgccgccgcg catgccgcca
     5281 gccagttcca gcagcgtgga gaacggcgtg cccatcggaa tctcgtaatt gcccggacgc
     5341 tcgacgtcgc ccgtgatcga gaacagcttg gtgccgccgt tgttcggctt gccgacttcc
     5401 aggtaggcct ggccgccgtt gcggatgatc cagggcaccg ccgcgaaggt ttcggtgttg
     5461 ttgatggtgg tgggcttgcc gtacaggccg aagctcgccg gaaacggcgg cttgaagcgc
     5521 ggctggccct tcttgccctc gagcgactcg agcaatgcgg tttcttcgcc gcagatatag
     5581 gcgccgtagc cgtggaaggc gtgcagctgg aaattgaaat ccgaacccag gatcttgtcg
     5641 cccaggaagc ccgcggcgcg cgcctcgtcg agcgcctctt cgaagcgttc gtacacttcg
     5701 aagatttcgc cgtggatgta gttgtagccg acggtgatgc ccatggcgta ggcggcgatg
     5761 gccatgcctt cgatcacgat gtgcgggttg aagcgcagga tgtcgcgatc cttgaacgtg
     5821 ccgggttcgc cttcgtcgga gttgcagacc aggtacttct gacccggaaa ggtgcgcggc
     5881 atgaagctcc acttcaggcc ggtgggaaag cccgcgccgc cgcggccgcg caggcccgag
     5941 gccttgactt cggcgatcac gtcttcgggc ttcatgcccg tggtgaggat cttgcgcagc
     6001 gcctcgtagc cgccacgctt gacgtagtcg gccaggcgcc agttggcgcc atcgacgtcg
     6061 gccatgatct gcggcttgat gtgccggccg tgcagggcca tcgagttggc cagatcattg
     6121 aagggatcgg gatccaggcc ttgggacaag gattggtaca gggattgttc cagggcgctc
     6181 atgcggcttc ccctttcttc agcatgtcga ccagcgcatc gagcttttct tcggtcatgc
     6241 gcacgcacat atgcttgttg ttgaccagca gcaccggcga gtcgccgcag gcgcccatgc
     6301 actcgccctc gaccagggtg aactggccgt cggccgtggt ttcactgtag tcgatgccca
     6361 gtttgcgctt gaggtagtcg cccgcgcgtt cgcccgaacg cagcgcgcaa ggcaggttgg
     6421 tgcacaccga gagcttgtgc ttgcccaccg gcgcgacgtt gaacatgttg tagaaggtgg
     6481 agacttcctc cacggcgatc ggctccacgc cgatgtactt cgccacgtct tcgaggatgt
     6541 cgggagacag ccagcccttt tcgtcttgcg cgatggcaag cgcggccatg atggcggact
     6601 gccgttggtc ggccgggaac ttggcgagtt cccgatcgat tttctggtag gcctgtgcag
     6661 aaagcagcat agttggggaa tccagatata tgcgtggtcg ttgacctggc gcggggctca
     6721 gcggtcgacc tcaccgaaaa cgatgtcctg cgtgccgatg atggtgacgg cgtcggcgat
     6781 catgtggccg cgggtcattt cgtcaagcga ttgcaggtgc gcaaagcccg gcgcccggat
     6841 cttcaggcgg taaggcttgt tggcgccgtc ggacaccaga tagatgccga actcgccctt
     6901 ggggtgctcc accgtggagt acacctcgcc cggcggcacg tggaaacctt ccgtgaagag
     6961 cttgaagtgg tggatcagct cttccatgtt ggtcttcatg ccgacgcgat ggggcggcgc
     7021 gactttatgg ttgtcgctca tcaccggacc gggattgttg cgcaaccact ccacgcattg
     7081 gcggacgatg tggttgctct ggcgcatttc ggcgacacgg accaggtagc ggtcatagca
     7141 gtcgccgttg acacccaccg ggatgtcgaa gtcgagcagg tcatagactt cgtagggctg
     7201 ggtcttgcgg gtatcccagg cgatgcccga gccgcgcagc atcgggccgg tgaagcccag
     7261 ggccttggca cgatcgggat cgaccacgcc cacgcccacc aggcgctgct tccagatgcg
     7321 gttgtcggtg agcagcgttt cgtactcgtc gacgcaggcc gggaagcggt tggtgaagtc
     7381 ttcgatgaaa tcgagcagcg aacccgagcg ggcctggttg acgatgtgga tgtctttttc
     7441 gctgcggtac ttggtgctgc cctggaactg cgccatggtg tcgggcaggt cgcggtagac
     7501 accgcccggg cggtagtagg ccgcgtgcat gcgggcgccc gagacggctt cgtagcagtc
     7561 catcaggtct tcgcgttcgc ggaaggcata caggaagacc gccatggcgc ccacgtccag
     7621 cgcgtgcgaa cccaccgaca tcaggtggtt gaggatgcgg gtgatctcgt cgaacatcac
     7681 gcggatgtac tgcgcgcgca acggcacctc gacgcccagc agcttctcga tggccatgac
     7741 gtaggcgtgc tcgttgcaca tcatcgagac gtagtcgagg cggtccatat agggaagcgc
     7801 ctggagatag gtcttgtgct cggccagctt ttcggtggcg cggtgcagga ggccgatgtg
     7861 cggatcggcg cgctggatga cttcgccgtc gagttcgagc accaggcgca gcacgccgtg
     7921 cgcggacggg tgctgcgggc cgaagttgag cgtgtagttc ttgatttctg ccatgattga
     7981 tcgctatcag cgccccatgc cgtaggtgtc ttcacgcacg acgcgcggcg tgatctcgcg
     8041 cggatcgatg gtgacgggct ggtagacgac gcggtgctgc tcgctgtcgt agcgcatttc
     8101 caccgtgccg gacaggggga aatccttgcg gaaagggtgg ccgatgaagc cgtagtcggt
     8161 caggatgcgg cgcaggtcgg ggtggccttc gaagacgatg ccgtacaggt cgaaggcttc
     8221 gcgctcgaac cagttgacgc tgggccagca ctcgaccagc gaggccacca tggggaactc
     8281 gtcgtcggga gcccaggtgc gcacgcgcag gcgccagttg tgttcgaaag acagcaggtg
     8341 caccaccacg gcgtatcggc caccgtgcgg gccgcgctgg atgcctgcac cctgctcttc
     8401 gcgctcgatg cgcgtggcat tgccgtaggt caggtagtcg acgccgcaca gatcgaggca
     8461 ggactcgaag cgcaggccgg catcggtacg cagcttggtg cacgccgaga accattgttc
     8521 ggccggtact tcgagggtca gctcaccgaa ggcttcggtg attgcagctt cctggccaaa
     8581 aacggcctgc aggtgggttt tcagggtttc gagcctggtc gtcatatcgg attcagcgcg
     8641 caatggtgtt ggtcaggcgg atcttgttct gcatttgcag caggccgtaa accagcgctt
     8701 cggccgtggg cggacagccg ggcacgtaga cgtcgaccgg cacgatgcgg tcgcagccgc
     8761 gcaccacgga atacgaatag tggtagtagc cgccgccatt ggcacaggaa cccatggaca
     8821 cgacccagcg cggctcgggc atctggtcgt agaccttgcg cagggcgggc gccattttgt
     8881 tgcacagcgt gccggccacg atcatcaggt cggactggcg cgggctgggg cggaagatga
     8941 tgccgaactg gtccagatcg tagcgcgccg cgccggcgtg catcatttcg accgcgcagc
     9001 aggccagacc gaaggtcatg ggccacatcg aaccggtctt ggcccagttg atgaatttat
     9061 cggcgctggt ggtgatgaaa ccttgcttga ggatgccgtc tatagccatg gtagttctcg
     9121 actccgggtg cgtgtatggg ctgggtaagc gatgcgggcc agggaggaca cgccccggcc
     9181 gggccgccta ctcccaatca agcgcgccct ttttccattc gtagatgaag ccgaccgtca
     9241 gcacggccag gaagaccatg acggtccaga agccggcgag gcccagagtg ccttgggcaa
     9301 tggcccaagg aaagaggaag gcgatttcca ggtcgaagag gatgaagagg atggccacca
     9361 ggtagtagcg cacgtcgaac ttcatccgcg cgtcttcgaa agcctcgaaa ccgcactcat
     9421 agggggaaag cttttctgca taaggacgct tggggccgag cagggaacca gctgtaatca
     9481 gtgcaaaccc gatcagggtt gccaccagga taaacagcag gacggggaaa tactgttgca
     9541 ggttcattag tcgtccgtcg tcgatgcaca accacgagat tgtagcactc agccggttac
     9601 gttcggctga cttcacttac gttcatgagg gtctggcgca ggcgagattc aagcataaaa
     9661 aaaccgctct ttcgagcggt tttttagcaa gcaggctatg acgatgcctg ctcgatgagc
     9721 catttactca aaccaagagc ggggttatgc accccgcgca agatcagaac tggtggcgga
     9781 tgccgacttc ggccagggtg gccttttggc cattgacgtt gttcagaccc ttgatgtaac
     9841 cggcggcggc gtacaggttg gtacgcttgg acaggttgta ggtcgcgccc agggcgaagg
     9901 tgttctgggt ggtcgtgccc aga
//
LOCUS       DASWPO010000343        11014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1422905, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000343 DASWPO010000000
VERSION     DASWPO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422905"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /gene="ddpX"
                     /locus_tag="VGC69_18275"
     CDS             complement(<1..408)
                     /gene="ddpX"
                     /locus_tag="VGC69_18275"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEY0297280.1"
                     /translation="MSAHSLVLITPDTHGVEIDLAYATADNFIGKVVYRHAHCLLLAQ
                     AEAALRRAVDIARQAGYTLRIFDAYRPPQAQQALWDFLPDARYVADPRRGSNHGRGTA
                     VDLTLVDAGGQALDMGTGFDDMTAASGHFHPGLP"
     gene            552..1235
                     /locus_tag="VGC69_18280"
     CDS             552..1235
                     /locus_tag="VGC69_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0297281.1"
                     /translation="MDSAPATAASSPIPARARPHAGARRGVFIKWLRKTHGWLGLWGA
                     VLGLMFGTSGFLQNHRAIMKINTPAPAVTTVRVQVPESVAQTPEEVGAWLQQQWGLAK
                     PMERAMRTPGREVSWGGRAMPQSEHWQLMFRTPRDVIQVEYSPDTREASARRSAPGWL
                     GVIENLHKSNGVGVAWVLMADSFAGSLVLLSLTGLLLWTELERKKLVGATVFVVSVVT
                     LLVVTAVTL"
     gene            1320..1586
                     /locus_tag="VGC69_18285"
     CDS             1320..1586
                     /locus_tag="VGC69_18285"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0297282.1"
                     /translation="MIGVWPSAGSFRDLQAMAEGVARMMDVVGADHVGIGTDMLGFIS
                     PPVFRSYAQLPALAAALRKAGLSDGDIGKVLGGNYRRVFEASVG"
     gene            complement(1627..3657)
                     /locus_tag="VGC69_18290"
     CDS             complement(1627..3657)
                     /locus_tag="VGC69_18290"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY0297283.1"
                     /translation="MLLLDKQAWIHSAKSLLAAVIALYIALAADLSRPYWAMTTAYVV
                     LQPVMGGTNSRGIYRITGTLIAAVAVVMFVPGLIQAPIVLSLAMSLWLAGCIFVALLH
                     RGPSSYVFMLAGYTAAFVGFPMVLQPDAIFATALARSEEIILGSLCAVVVGALVFPTS
                     IKPMIGQRVQALMNDARSWCAQVLAHRGSSDELRRRLAKDLSQLDLVIPFTKRDDPRH
                     GNLNEWLSELRARLLGMLPVLAAIEDRLDNVGADAAQDAELERLIGDMRVWISEDAVP
                     AVQTLRDFRQRIDALRPALDDGSDGLLRNSLLLRLKELTELWYDSRHLQQSILDGAPP
                     PEPAFGLDLRRLVRVDNRHIDWSMLTFSSLAAGVTLFTYCLLWIALGWPEGVSGAMLA
                     AVAAAFFAAQDDPSPNILAFMIWAIVANLTAGIYLFGILPAFHDFVPLVLVLTPTFLL
                     VGLMASRPRLFLPGMILITNMASLLSIQNHSAVDFTSYVNASVSTIIGLLFALVMTRL
                     FRSVGAEWSAQRLIRQGWRLIATAAEGSGRQDRDRFLVRMLDLLGLLAPRLASLPAGS
                     DAAGVDMLDEVRIGLNVLNLRRARNDLPEANQDALNQLLDGVATHYRARERAGRPLPP
                     PQELKTALETSLSRLRTLPPGDMRDEALLGLIGLRIGLFPQQYLPGALDERP"
     gene            complement(3669..5153)
                     /locus_tag="VGC69_18295"
     CDS             complement(3669..5153)
                     /locus_tag="VGC69_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0297284.1"
                     /translation="MNARSTRAMVLGPLLASLASCATVGPDYTLPQDSAFAKAAQHPP
                     ALDVRGSTAVDPAQAAVEGRWWALYDDAQLNALVEQALQANTELKAAAAHLTQAQARY
                     AQARAAGGLSEQVDASVARGRISAQSLLQTEPLPIFNFADGGISVSYQLDLFGKIRRG
                     AEAARASAEAAQAAGDLARISVAASVVGAYAEICHSNHELAVARHSLQLQQRSRDVAA
                     RLQAAGRGTPAAVARADSQVATLQAALPPLESRKLAAGYELAALLGRTPDQVPAEALQ
                     CQDAPMLKQAIPLGSGAALLRRRPDVRRAERELAAATAEIGIATADLYPDIRLGASFG
                     ANGVLENFGQSITQEWSFGPLISWSIPGNGAHARVALAKAGAEAALAEFDHVVLEALR
                     ETQTILGAYAEDLRRVAALREARDRASAAATDARRLYQAGREPYLSSLDGERTLAAAE
                     TSLAGAEAQVSQDQIRLFLALGGGWRKNEPPHADDDAAPSPGKP"
     gene            complement(5150..6142)
                     /locus_tag="VGC69_18300"
     CDS             complement(5150..6142)
                     /locus_tag="VGC69_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0297285.1"
                     /translation="MNPLTRKIVRIVPTALLVVAAILVLRHLWQYYMLDPWTRDAHIS
                     ADVVQVAPDVSGPVLEVRIHDNAPVHRGDVLFVVDQERYKIALKQAQAVLAQSQAALA
                     QSQAALLQNQTTQRQLQREAARDHALKDLVATEEAETRRANLEKAQAGVAAAEASVAA
                     AQASIATSQAAIDLAQLNLDRTIVRSPVDGRVNDRTVRVGDYVSASKPVLAVLDTGSF
                     RIDGYFEETRLHGIREGDPVDIHIMGEDGVLHGHVQSIAAGIEDRYRSEGSSLLPNVT
                     PAFDWVRLAQRMPVRISLDAVPDGVHLIAGRSVTVSVVGGDETRKHAARPASQS"
     gene            complement(6139..6348)
                     /locus_tag="VGC69_18305"
     CDS             complement(6139..6348)
                     /locus_tag="VGC69_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEY0297286.1"
                     /translation="MYGEFSVYGVFIPTLMGLMLMAYLVLNGLRFLLLKAGAYRHIWH
                     PPLFNFALYILVLGALFALMRNMLA"
     gene            complement(6368..6856)
                     /locus_tag="VGC69_18310"
     CDS             complement(6368..6856)
                     /locus_tag="VGC69_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297287.1"
                     /translation="MSRTMTEVDRILFDFTIAIQPARRAWVQAVGTALTDDKLPMSLA
                     VVIVMTSRMGAAAHQRALAAEIGINPAALVRVLDQGESAGLLKRCDVPGNRRTKTVEL
                     LPLGKALAEKMETALTALRSRMLHDIPAKDVATATRVLRALEAESLAYVTATGSTNKF
                     DE"
     gene            complement(6950..7783)
                     /locus_tag="VGC69_18315"
     CDS             complement(6950..7783)
                     /locus_tag="VGC69_18315"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0297288.1"
                     /translation="MARTWFITGASRGLGLETARAALIAGDQVVATARQVGHIRQELA
                     GHGDRLLAAALDVTDYASVQAAVQAAMRQFGRIDVLVNNAGYGQFGAFEELSVRAINR
                     QFETNVFGTFRVTRAILPIMRAQRAGHIMTISSIAGISGWDGSSIYCASKFALAGWSE
                     SLSLELARFNIQATSVHPGFFRTDFLDQSSARYGDIEMDDYRESSAQLNRRRAERNHK
                     QEGNPERFGVAMVQLSRLKKPPVRFGAGTDSVEAMLGRAESLLTATLEWRALSEATDF
                     T"
     gene            7965..8903
                     /locus_tag="VGC69_18320"
     CDS             7965..8903
                     /locus_tag="VGC69_18320"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0297289.1"
                     /translation="MLETSLFGSRGKLLAKIDSLTRSAEDLMTPIPGLTLYRRTHATP
                     PRAVMYGPGLAIVVNGRRRTIVEDQAHVFDASHFLLTAIDLPMITEIVEASREAPYLS
                     LLLKLDLPMVRELMTEMDMLDPGAGPQDVGTAIGPMSDSIFEDVGRLLELLETPRDIP
                     ILGRLIQRGIFYRVLAGPAGGRLRQIVRAGMPGGRIARAIEWLRSNYLQPLQIEELAA
                     VAHMGVSTLHHQFRARTSMSPLQYQKRLRLHEARRILLGEDIDAKAVAMRVGYESNAQ
                     FNREYRRLFGAPPVTDIKALKHAGADTAAGGKRIAA"
     gene            8994..9944
                     /locus_tag="VGC69_18325"
     CDS             8994..9944
                     /locus_tag="VGC69_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0297290.1"
                     /translation="MTASKDHKSGAPGYNVAWTGGEEHWIAREPGIDLFLWHKPRAPG
                     TEYVGTILFVHGSSMASQPTFDLTVAGRPDSSAMDWFRARGFDTWCLDNEGYGRSSKH
                     RDINFDIPNGADDLAAASEYIIGVTGARALMVYGISSGALKAALFAQRHPERVAKLAL
                     DAFVWTGAGSRTLAQRAKRMSEWTSANRRPIDLDFVRSIFNRDHPDTTEARVVEAFAA
                     GILTLDSSVPTGTYVDMCSRLPLVDPDKLVVPTMVMRGEYDGIASFQDLIDFFCRLPN
                     NDKQFSVMAGISHASFQQKNYRSVYHILHGFFAAPARVYG"
     gene            complement(10001..>11014)
                     /locus_tag="VGC69_18330"
     CDS             complement(10001..>11014)
                     /locus_tag="VGC69_18330"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0297291.1"
                     /translation="YPSPACVDPYHVARKAALAGQVAPSTSVIVDRLLNLDASMDVQV
                     MAATLDSGYDCRVAGADRLNVPKTSGYAPCLLVGDMIFVAGQLARDAGGNIAAQATVP
                     PGQQWNGTRIKLETDYLVRERLLPALAVADSDLSLVLKAQVYLSHAEDLPAFWQAWSS
                     AFGGRVPPTTVVPVNHPAFGNRDATIEVNLVAVPKSAAGRVRDIECDVDLIAGDMLPA
                     RVFDGVLFVAGLMAVEDGGLCGLAAVPASMPFFHDSTQAQMADILDKAGRIFSAAGSN
                     LDHVVRALHFHADLAQFRSGFMAWDESLRRQGIPFSAIGVGQDMFVPGASVMLDLWGH
                     VPG"
BASE COUNT         1798 a         3623 c         3804 g         1789 t
ORIGIN      
        1 gggcaggccg ggatggaaat ggcccgaggc cgcggtcatg tcgtcgaatc ccgtgcccat
       61 gtccagcgcc tgtccgccgg cgtcgaccag cgtcaggtcc accgccgtgc cccggccgtg
      121 gttggagccg cgcctggggt cggccacata gcgcgcgtcg ggcaggaaat cccacaatgc
      181 ctgctgcgcc tgcggcggcc ggtaggcatc gaagatgcgc agcgtatagc cggcctggcg
      241 ggcgatgtcc acggcgcggc gcagggctgc ttcggcctgc gccagcagca ggcagtgcgc
      301 atggcggtag acgaccttgc cgatgaaatt gtcggcggtg gcgtaggcca ggtcgatctc
      361 gacgccgtgg gtgtcggggg tgatgaggac gagggaatgg gcgctcatgg cggtgtgcgg
      421 cgttcggtgc atgggtcagg gcaaagtgta gggtgcatcg taccgcctgc cgctgagggc
      481 tgaccctggc ttgcgcgctt gtgtccgctc attcaataat gagagctatt cttgattaat
      541 tagacgcccc catggattcc gcacccgcta ccgccgcttc gtctcccatt cccgcccggg
      601 ccaggccgca tgccggcgcg cgccgtggcg tgttcatcaa gtggctgcgc aagacgcacg
      661 gctggctggg cctgtggggc gccgtgctgg gcctgatgtt cggcaccagc ggcttcctgc
      721 agaaccatcg cgcaatcatg aagatcaaca cgcccgcgcc ggccgtcacc accgtgcgcg
      781 tgcaagtgcc ggagtcggtt gcgcagacgc cggaagaggt aggggcctgg ctgcagcagc
      841 aatgggggct ggccaagccg atggagcgcg ccatgcgcac tcccggccgc gaggtgtcct
      901 ggggcggccg cgcgatgccg cagtccgagc attggcagtt gatgttccgc acgcccaggg
      961 acgtgatcca ggtcgagtat tcgcccgaca cccgcgaggc ctcggcccgc cgcagcgcgc
     1021 cgggctggct gggcgtgatc gagaacctgc acaaatccaa cggcgtcggc gtggcctggg
     1081 tgctgatggc ggattccttc gccggcagcc tcgtcctgct gtcgctgacg ggcctgctgc
     1141 tgtggacgga gctggaacgc aagaagctgg tgggcgcgac ggtgttcgtg gtgtcggtgg
     1201 tgacgctgct ggtggtgacg gcggtgacgc tatgaagcgc tgacggcccg ggccatgacg
     1261 ctcgactggg aatcgatccg taccggcgcg gccgccacgc cgtcgcggat acgggcggcg
     1321 tgatcggcgt gtggccgagc gcgggctcgt ttcgcgacct gcaggccatg gccgagggcg
     1381 tggcgcggat gatggacgtg gtcggcgccg atcacgtcgg catcggcacc gacatgctcg
     1441 gcttcatctc gccgcccgtg ttccgcagtt atgcgcagct ccccgcgcta gcggccgcgc
     1501 tgcgcaaggc ggggctgtcc gacggcgaca tcggcaaggt gctgggcggc aattaccggc
     1561 gcgtgttcga ggcgtcggtc ggctaggcga tagagccggc ctgcccgccc cttgccttgt
     1621 tttgcgtcag ggccgctcgt ccagcgcgcc gggcagatac tgctgcggga acaggccgat
     1681 gcgcaggccg atcaggccca gcaaggcctc gtcccgcatg tcgcccggcg gcagcgtgcg
     1741 caggcgcgac agcgaagtct ccagcgccgt tttcagttcc tgcggcggcg gcagcggccg
     1801 gccggcccgt tcgcgcgccc gataatgggt ggcgacgcca tccagcagct ggttcagggc
     1861 gtcctgattg gcttcgggca ggtcattgcg cgcgcggcgc aagttgagga cgttcaggcc
     1921 gatgcgcacc tcgtccagca tgtccacgcc ggcggcgtcg ctgccggctg gcagcgacgc
     1981 caggcgcggc gccagcaggc cgagcaggtc cagcatgcgc accaggaaac ggtcgcggtc
     2041 ctggcggccg ctgccctcgg cggcggtggc gatgagccgc catccctggc ggatcagccg
     2101 ctgcgcgctc cattcggcgc cgaccgagcg gaacagccgc gtcatcacca gggcgaacag
     2161 caggccgatg atggtcgaca ccgaggcgtt gacgtagctc gtgaaatcca ccgcggagtg
     2221 gttctggatc gacaacagcg acgccatgtt ggtgatcagg atcatgcccg gcaggaacag
     2281 gcgggggcgg gacgccatca gtccgaccag caggaaggtg ggcgtgagga ccagcaccag
     2341 cggcacgaag tcgtggaacg ccgggaggat gccgaacagg tatatgcccg cggtcaggtt
     2401 cgccacgatg gcccagatca tgaacgcgag gatgttcggc gacggatcgt cctgcgcggc
     2461 gaagaaggcg gcggccaccg cggccagcat tgcgccggat acgccctcgg gccagcccag
     2521 ggcgatccac agcaggcaat aggtgaacag cgtgacgccg gcggccagcg acgagaacgt
     2581 cagcatgctc cagtcgatat ggcggttgtc gacgcgcacc agcctgcgca ggtcgagacc
     2641 gaaggccggc tcgggcggcg gcgcgccgtc caggatcgat tgctgcagat ggcggctgtc
     2701 gtaccagagc tcggtcaatt ccttcaggcg cagcagcagg ctgttgcgca acaggccatc
     2761 gctgccgtcg tccagggccg gacgcaatgc atcgatgcgc tggcggaagt cccgcagcgt
     2821 ttgcaccgcc ggtacggcat cctcgcttat ccatacgcgc atgtcgccga tgaggcgctc
     2881 gagttcggcg tcctgggcgg cgtccgcgcc gacattgtcg agacgatctt cgattgcggc
     2941 cagcaccggc agcatgccga gcaggcgcgc gcgcagctcc gatagccatt cgttaaggtt
     3001 gccgtggcgc ggatcgtcgc gcttggtaaa cgggatcacc aggtccagtt gcgaaaggtc
     3061 cttggccagg cgccggcgca gttcgtcgga actgccgcgg tgcgccagca cctgcgcgca
     3121 ccaggatctt gcgtcgttca tcagggcctg gacgcgttgt ccgatcatcg gcttgatcga
     3181 ggtcggaaac accagggcgc cgaccacgac cgcgcacagg ctgcccagta tgatctcctc
     3241 gctgcgcgcc agcgccgtcg cgaagatcgc atcgggctgc aacaccatgg ggaagccgac
     3301 gaacgcggcc gtgtagccgg ccagcatgaa cacgtatgac gacggcccgc gatgcagcag
     3361 cgcgacgaag atgcaccccg ccagccagag cgacatcgcc aggctcagta cgatcggtgc
     3421 ctggatcagc ccgggaacga acatcaccac ggctaccgcc gcgatcagcg tgccggtgat
     3481 acggtagatg ccgcgcgagt tcgtgccgcc catcaccggc tgcagcacga cgtaggcggt
     3541 ggtcatggcc caataaggcc gcgacaagtc agccgcaagc gcgatataca acgcgatcac
     3601 cgcggccagg agcgatttgg ccgagtgtat ccatgcctgc ttgtcgagca gcaacaagat
     3661 gcggttcctt aaggcttgcc cggagacggc gccgcatcgt catcggcatg cggcggctcg
     3721 ttcttgcgcc agccgccgcc cagggccagg aacaggcgga tctggtcctg cgagacctgc
     3781 gcttcggcgc cggccaggct ggtttcggcg gccgccagcg tgcgctctcc gtccaggctg
     3841 gacaagtacg gttcgcggcc ggcctggtac aggcggcgcg cgtccgtggc ggcggcgctg
     3901 gcgcggtcgc gggcttcgcg caacgcggcc acgcggcgca aatcctcggc gtaagcgccg
     3961 agtatggtct gggtttcgcg cagcgcctcc aggacgacgt ggtcgaattc ggccagcgcc
     4021 gcctcggctc ccgccttcgc cagcgcgacc cgcgcgtgcg cgccattgcc gggaatcgac
     4081 catgagatca gcgggccgaa cgaccattcc tgggtgatcg attggccgaa attctccagc
     4141 acgccattcg cgccaaacga agcgccaagg cgaatgtccg gatataggtc ggcggtggcg
     4201 atgccgattt cggcggtggc ggcggccagc tcgcgttcgg cgcggcgcac gtcgggacgg
     4261 cggcgcagca gcgcggcgcc gctgcccagg ggaatggcct gcttcagcat cggcgcgtcc
     4321 tggcattgca gcgcctcggc gggaacctgg tcgggggtgc ggccgagcag cgcggccagt
     4381 tcgtagcccg cggccagctt gcgcgattcc agcggcggca gcgccgcctg cagcgtcgct
     4441 acctgcgaat cggcacgcgc caccgccgcc ggcgtgccgc ggccggccgc ctggaggcgc
     4501 gcggcgacgt cgcggctgcg ctgctgcagc tgcagcgaat ggcgcgccac ggccagttca
     4561 tggttgctgt ggcaaatctc ggcgtaggcg ccgaccaccg acgcggcgac gctgatgcgc
     4621 gccaggtcgc cggcggcctg cgcggcctcg gcgctggcgc gcgcggcttc ggcgccgcgc
     4681 cggatcttgc cgaacaggtc gagctggtag gacaccgaaa tgccgccgtc ggcgaagttg
     4741 aatatcggca gcggttcggt ctgcagcagc gactgggcgg aaatccggcc gcgcgcgacc
     4801 gaggcatcga cctgctcgct caggccgccg gcggcgcgcg cctgcgcgta gcgcgcctgg
     4861 gcctgggtca ggtgcgcggc ggcggccttc agttcggtat tggcctgcag ggcctgctcg
     4921 accagcgcgt tgagctgcgc gtcgtcgtaa agcgcccacc agcggccttc gacggcggcc
     4981 tgcgccgggt cgacggcggt cgagccgcgc acgtccagcg cgggcggatg ctgcgccgct
     5041 ttggcgaaag ccgagtcctg cggcagcgtg tagtccgggc ccacggtcgc gcatgaggcc
     5101 agcgaggcca gcagcgggcc caggaccatg gcgcgcgtgc tgcgggcgtt catgattggc
     5161 tggccggccg cgcggcatgc ttgcgcgtct cgtcgccgcc gacgacgctg accgtgacgc
     5221 tgcggcccgc gatcaggtga accccgtccg gcaccgcgtc caggctgatc cgcaccggca
     5281 tgcgctgcgc cagccgcacc cagtcgaagg ccggcgtgac gttcggcagc aggctggagc
     5341 cttcgctgcg atagcggtcc tcgatgccgg cggcaatgct ttgcacgtgg ccgtgcagca
     5401 cgccgtcctc gcccatgatg tggatgtcca ccggatcgcc ttcgcggatg ccgtgcaggc
     5461 gggtttcctc gaaatagccg tcgatgcgga acgaccccgt atccagcacc gccagcaccg
     5521 gcttgctggc gctcacgtag tctccgacgc gcaccgtgcg gtcattgacg cggccatcga
     5581 ccggactgcg cacgatggtg cggtccaggt tcaattgcgc gagatcgatc gcggcctgcg
     5641 aggtggcgat gctggcctgg gcggcggcga cgctggcttc ggcggcggcg acgccggcct
     5701 gcgctttttc caggttggcg cgccgagtct cggcttcctc ggtggccacc aggtccttca
     5761 gcgcgtgatc gcgcgccgcc tcgcgttgca gctggcgctg cgtggtctgg ttctgcagca
     5821 aggccgcttg gctctgcgcc agggcggcct ggctttgcgc cagcacggcc tgcgcctgtt
     5881 tcagcgcgat cttgtagcgc tcctggtcga cgacgaacag cacatcgccg cgatgcaccg
     5941 gggcgttgtc gtgaatccgc acctcgagca ccggcccgga gacatccggc gccacctgca
     6001 ccacgtcggc gctgatatgc gcgtcgcggg tccaggggtc cagcatgtag tactgccaca
     6061 agtggcgcaa caccagaatg gcggcgacga ccagcagcgc ggtcggtacg atcctgacga
     6121 tcttgcgagt gaggggattc atgccagcat attgcgcatc agcgcgaaca gggcgcccag
     6181 taccaggatg tacaaggcga aattgaacag ggggggatgc cagatatggc ggtatgcgcc
     6241 tgctttcaag agcaagaagc gcaggccgtt gagcaccagg taggccatca gcatcaggcc
     6301 catcagcgtg gggatgaaga cgccgtagac gctgaattct ccgtacatga cagtggctgc
     6361 ggttttttta ttcgtcgaac ttgttcgtcg aacccgtcgc cgtgacgtac gccagcgatt
     6421 ccgcctccag cgcgcgcagc acccgggtcg cggttgcgac gtctttggcc gggatgtcgt
     6481 gcagcattct gctgcgcagc gccgtcagcg ccgtctccat cttctcggcc agcgccttgc
     6541 ccaggggcag cagctcgacc gtcttcgtcc ggcgattgcc gggaacgtcg cagcgtttca
     6601 gcaggccggc cgattcgccc tggtccagca cgcgcaccaa tgccgccgga ttgatgccga
     6661 tctcggcggc cagcgcccgc tggtgcgcgg cggcccccat gcgggacgtc atcacgatga
     6721 ccacggccaa tgacatgggc agcttgtcgt cggtcagcgc ggtgccgaca gcctggaccc
     6781 atgcccttct ggcgggctgg atggcgatag tgaagtcgaa gaggatgcgg tcgacttcgg
     6841 tcatggtgcg ggacatatag atgataaagc aatcattgct ttttgaattg taatcgtatc
     6901 tactatctgg cacaagcccg atggatccga ggccgcggcc gagcggtctc tacgtgaaat
     6961 cggttgcttc ggacaaggcg cgccattcca aggtggcggt gagcagcgat tcggccctgc
     7021 cgagcatggc ttccacggaa tccgtgccgg ccccgaagcg cacgggcggc ttcttgagcc
     7081 gggacaactg aaccatcgcc acgccaaacc tttcgggatt tccttcttgc ttatggttgc
     7141 gttccgccct gcgccggttg agttgcgcgc tcgactcccg gtagtcgtcc atctcgatgt
     7201 ccccgtaccg ggcggagctc tggtccagga aatccgtgcg gaagaaaccg gggtggacgg
     7261 atgtcgcctg gatgttgaag cgggccagtt cgaggctcaa ggactcggac cagccggcga
     7321 gcgcgaattt ggaggcgcaa tagatggaag aaccatccca gccgctgatg ccggcgatgg
     7381 acgagatggt catgatgtgc ccggcgcgtt gcgcgcgcat gatcggcagg atggcccggg
     7441 tgacccggaa ggtgccgaac acattggttt cgaattggcg gttgatcgcc cggacggaga
     7501 gttcctcgaa ggcgccgaac tgtccgtacc cggcattgtt gacgagcacg tcgatacggc
     7561 cgaactgccg catcgccgcc tgcaccgcgg cctggaccga ggcgtagtcg gtgacgtcca
     7621 gggccgcggc aagcagccgg tcgccgtggc cggccaattc ctgccggatg tggccgacct
     7681 ggcgagcggt cgccacgacc tggtcgcccg cgatcaacgc ggcgcgcgcc gtctccagcc
     7741 ccaagcccct gcttgctcca gtgatgaacc aggtcctcgc catcgtttct ccgacaaagc
     7801 agggggccgc atcctgcggc ccaccgctcg aagactagaa cggcgcgggg ttcatcgcca
     7861 agacatgatt cttcccattt cttgcctaat ccttctttcc gcggtccgaa ggcgaaattc
     7921 ccccgcttgc gcaccacggc gtttgaccga aagaaacgct cgccatgctt gagacatcat
     7981 tgttcggttc gcgcggcaaa ctgctcgcca agatcgattc gctgacccgg agcgcggaag
     8041 acctgatgac gccgattccg ggcctgacgc tttatcggcg gacccatgcc accccgccca
     8101 gggccgtgat gtacgggccc ggcctggcca tcgtcgtcaa cggcaggagg cggaccatcg
     8161 tcgaggacca ggcgcatgtg ttcgatgcgt cccatttcct gctgacggcc atcgacctgc
     8221 cgatgatcac ggagatcgtc gaggcgagcc gcgaggcgcc gtacctctcg ctgctgctca
     8281 agctcgacct gccgatggtg cgcgagctga tgacggaaat ggacatgctc gatcccgggg
     8341 ccgggccgca ggacgtgggc acggcgatcg ggccgatgtc cgactcgata ttcgaggacg
     8401 tgggccggct gctcgaattg ctggaaacgc ccagggatat tccgatattg ggccggctta
     8461 tccaaagagg gatcttctac cgtgtcctgg ccgggccggc gggcggacgg ctaaggcaga
     8521 tcgtccgtgc gggcatgccg ggcggacgca tcgccagggc gatcgaatgg ctgcggagca
     8581 attatctgca gccgctccag atcgaggagc tggccgcggt ggcccatatg ggcgtatcga
     8641 cgctgcatca ccagtttcgc gcgcggacat cgatgtcgcc cctgcaatac cagaagcggc
     8701 tgcgcctgca cgaagccagg cgcatcctgc tcggcgagga catcgatgcg aaagcggttg
     8761 cgatgcgcgt gggctacgaa agcaatgcgc aattcaaccg ggagtaccgg cgcctcttcg
     8821 gcgcgccgcc cgtcacggat atcaaggcgt taaagcatgc cggcgccgat acggctgccg
     8881 gcggaaagcg gatcgccgcc tgacggtaaa cactctattc actaaaaact attgttcgcc
     8941 ccggccacat tggccaagat gctctcgaac cccttttcac tagagtatcg acgatgactg
     9001 cgagcaaaga ccataaatcg ggagccccgg gctacaacgt ggcctggacg ggcggagagg
     9061 agcactggat tgcgcgggaa ccgggcatcg acctgttcct gtggcacaag ccccgcgccc
     9121 cgggcacgga gtacgtgggg acgatcctgt tcgtgcacgg gtcgtcgatg gcgtcccagc
     9181 ccaccttcga cctgacggta gcagggcggc cggattcctc ggccatggac tggtttcgcg
     9241 cccgggggtt cgacacctgg tgcctggaca acgaaggcta cggccgttcc agcaagcacc
     9301 gtgacatcaa tttcgacatc ccgaacggcg ccgacgacct ggccgccgcg agcgagtaca
     9361 tcatcggcgt caccggcgcg cgggccttga tggtctacgg gatttcctcg ggcgccttga
     9421 aggcggcgct gttcgcgcag cgccatcccg aacgcgtcgc caagctggcg ctggacgcct
     9481 tcgtctggac gggggcgggc agccgcacgc tggcgcagcg cgccaagcgc atgtcggaat
     9541 ggaccagcgc caaccgccgt cccatcgacc tggatttcgt gcggagcatc ttcaaccgcg
     9601 accaccccga caccaccgaa gcccgcgtgg tcgaagcgtt cgcggccggc attctgacgc
     9661 tggacagctc ggtgcccacc ggcacttatg tggacatgtg ctccaggctg cccctggtcg
     9721 atccggacaa gctcgtcgtg cccacgatgg tgatgcgcgg cgagtacgac ggcattgcca
     9781 gtttccagga cctgatcgat ttcttctgcc ggctgcccaa caacgacaag caattctcgg
     9841 tgatggccgg catctcgcat gcgagcttcc agcagaagaa ttaccgcagc gtctatcaca
     9901 tcctgcacgg cttcttcgcc gcgcctgcgc gcgtctacgg ttgagccggg gccgggcgcg
     9961 aggcccggcc gccgtgatgg caaagcgcca gcccggcgga tcagccgggc acgtggcccc
    10021 acaggtcgag catcacgctg gcgcccggca cgaacatatc ctggccgacc ccgatggcgc
    10081 tgaacggaat gccctggcgg cgcaggctct catcccatgc catgaagccg ctgcggaact
    10141 gcgccaggtc cgcgtgaaaa tgcagcgcgc gcacgacatg gtccagattg ctgccggcgg
    10201 ccgagaaaat gcgcccggcc ttgtccagga tatcggccat ctgcgcctgc gtgctgtcgt
    10261 gaaagaacgg catgcttgcg ggaacagcgg ccaaaccgca caagcccccg tcttcgaccg
    10321 ccatcaggcc ggccacgaac agcaccccat cgaacacgcg ggccgggagc atgtcgccgg
    10381 cgatcaggtc gacgtcgcac tcgatgtcgc gcactcgtcc ggcggcggac ttgggcacgg
    10441 ccaccaggtt tacttcgatg gtggcatcgc gattgccaaa cgcgggatga ttgaccggca
    10501 ccacggtcgt gggcggcacg cggccgccga aggcgctcga ccaggcctgc cagaaagcgg
    10561 gcaggtcttc ggcatggctc agatacacct gggccttgag caccaggctc agatcgctgt
    10621 cggcgacggc cagggctggc agcagccgct cgcgcaccag gtaatcggtc tcgagcttga
    10681 tgcgcgttcc attccactgc tggccgggcg gcaccgtggc ctgggccgcg atattgccgc
    10741 cggcgtcgcg cgccagctgg cccgccacga agatcatgtc tcccaccagc aggcagggcg
    10801 catagcccga ggtcttgggg acgttcagcc ggtccgcgcc ggcgacgcgg cagtcgtagc
    10861 cgctatcgag cgtcgcggcc atgacctgca cgtccatcga cgcatccagg ttgagcaatc
    10921 gatcgacgat caccgacgtg ctgggagcga cctgtccggc cagcgcggcc ttgcgcgcca
    10981 catggtaggg gtccacgcac gccggcgagg gata
//
LOCUS       DASWPO010000344         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_260331, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000344 DASWPO010000000
VERSION     DASWPO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260331"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGC69_18335"
     CDS             <1..203
                     /locus_tag="VGC69_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY0297292.1"
                     /translation="MGYPEKVVGSRHFDLLAQVEDELILHVPYVPRHDVCPGAQASAA
                     VSEEPAERRPSPFAVLEQLKRK"
     gene            300..482
                     /gene="rpmF"
                     /locus_tag="VGC69_18340"
     CDS             300..482
                     /gene="rpmF"
                     /locus_tag="VGC69_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY0297293.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLVNPPSAVEPTTGEAHLRHHISP
                     NGFYRGRKVLKTKADE"
     gene            545..1609
                     /gene="plsX"
                     /locus_tag="VGC69_18345"
     CDS             545..1609
                     /gene="plsX"
                     /locus_tag="VGC69_18345"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY0297294.1"
                     /translation="MIRIAIDCMGGDAGLPVTIPAAVEFARQFPDTRLLLVGLPDQIE
                     AALAGQRHVPRDRIEIVPATEVVTMDDPVEVALRRKKDSSMRLAAQAVKDGRADACVS
                     AGNTGAWMAISRYVLKTLDGIDRPAIATSLPNQMGRATTVLDLGANVDCTAEHLLQFA
                     IMGSALAQALDHRDTPSVGLLNIGEEVIKGNEVVKEAAELLRGSQLNFYGNVEGNDIF
                     KGTVDVVVCDGFVGNVVLKAIEGLAKMLSGVIREEFKRNLLTLLAGALSIPVLNRLRK
                     RVDNRQYNGAALLGLRGVVIKSHGSADVYSYGFALQRAREAVASKLLERTAHAVAQIT
                     KREQVSGEVAGVPDEAGDKA"
     gene            1609..2595
                     /locus_tag="VGC69_18350"
     CDS             1609..2595
                     /locus_tag="VGC69_18350"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0297295.1"
                     /translation="MSTPMIYSAIAGTGGFLPERVVSNDALAAELAARGVETSDEWIV
                     ERTGIRQRHLAARGVTTSQLATEAARQALQDAGVEAGEVDLIILATSTPDYVFPSTAC
                     LVQANLGIAGGAAFDVQAVCSGFVYALTIADSFIRVGRARCALVIGAEVFSRILDWND
                     RKTCVLFGDGAGAVVLKASDKPGIMAAQLHADGSLAKILHAAGNVSHGEVTGDPFLRM
                     DGQAVFKQAVTVLERSARAVCDEAGLPVSAVDWLVPHQANLRIINFLGRKLGVPAEQV
                     VVTVDRHANTSAASVPLALDAARRDGRIKAGQTVLMQGVGGGFTWGSVLVRL"
     gene            2627..3577
                     /gene="fabD"
                     /locus_tag="VGC69_18355"
     CDS             2627..3577
                     /gene="fabD"
                     /locus_tag="VGC69_18355"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0297296.1"
                     /translation="MKIAFVFPGQGSQSVGMLDAWAGNAAVADVLARAGAALEQDLGA
                     LIAQGPAEQLNLTTNTQPVMLTAAYACYAAWTAAGGRKPDVMAGHSLGEYTALTAAGS
                     LALEDAVRLVRVRADAMQTAVPVGSGGMAAILGLDDDAVRAVCAQATASGQPGEVVEA
                     VNFNAPAQVVIAGSKAAVERACEAAKAAGAKRALPLPVSAPFHSSLLKPAADVLADAL
                     GKVQVAAPAIAVINNVDVAQAGDPAGIRDALVRQAWHPVRWVETIQAMKAQGVTHVVE
                     CGPGKVLAGLTKRIDGDLVGLTITDPASLEATLAALAAPQ"
     gene            3672..4412
                     /gene="fabG"
                     /locus_tag="VGC69_18360"
     CDS             3672..4412
                     /gene="fabG"
                     /locus_tag="VGC69_18360"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY0297297.1"
                     /translation="MSATELQGKTALVTGATRGIGRAIALELAGRGATVVGTATSESG
                     AQSVQEALAPLGGRGAVLNVTDAAACAALLDELARQGGPSILVNNAGITRDNLAMRMK
                     DEDWAAVIDTNLSAVFRLSRGVMRAMMKARWGRIINVTSVVGASGNAGQANYAAAKAG
                     VAGMARALARELGSRNVTVNCVAPGFIDTDMTRVLGEEQTAALTQQIPLGRLGAPEDI
                     AHAVAFLASPQAGYITGTTLHVNGGMYM"
BASE COUNT          727 a         1534 c         1479 g          702 t
ORIGIN      
        1 cgatggggta tcccgaaaaa gtggtgggtt cgcggcattt cgacctgttg gcccaggtcg
       61 aggacgaatt gatattgcac gtcccctacg tgcctcggca tgatgtatgc ccgggtgcgc
      121 aggccagcgc tgcggtttcc gaggaacccg ccgaacgccg cccgtcgccg ttcgcggtgc
      181 tcgagcagct caagcgcaaa tgaagtggta acccccggcc gtaaaacccg gccgtaatcc
      241 gcagtaattt ttttcgcggg cagcatcgcg tacaatgcgg cccgtttcta ggagtcatca
      301 tggctgttca acaaaacaag aaatccccgt ccaagcgtgg catgcaccgc tcgcacgatt
      361 tcctggtcaa tccgccttcc gcggttgagc ccacgaccgg cgaagcgcat ctgcgccatc
      421 acatcagccc caacggtttc taccgtggcc gcaaggtcct gaagaccaag gccgacgaat
      481 aactcgtcgc caatcttgcg gctctccgtg cgtagcactc aggccgaact gatccaagtt
      541 ttccgtgata cgcatcgcca tcgactgcat gggcggcgac gccgggctgc ccgtaaccat
      601 accggctgcg gttgagttcg ctcgccagtt tccggatacc cggctgctgc tggtcggcct
      661 gcccgaccag atcgaggccg ccctggccgg gcagcgccat gtgccgcgcg atcgcatcga
      721 gatcgtgccg gccaccgagg tcgtcaccat ggacgatccg gtcgaggtcg ccctgcgccg
      781 caagaaagac tcatccatgc gcctggccgc ccaggccgtc aaggacggcc gggccgatgc
      841 ctgcgtgtcg gccggcaata ccggcgcctg gatggccatc tcgcgctacg tgctcaagac
      901 gctggacggc atcgaccgtc ccgcgatcgc cacgtcgctg cccaaccaga tgggcagggc
      961 caccacggtg ctggacctgg gcgccaatgt cgactgcacg gccgagcatc tgctgcaatt
     1021 cgccatcatg ggctcggccc tggcgcaggc gctcgaccac cgcgacacgc ccagcgtggg
     1081 cctgctcaat atcggcgaag aggtcatcaa gggcaacgag gtcgtcaagg aagccgccga
     1141 gctgctgcgc ggcagccagc tcaacttcta cggcaacgtc gaaggcaacg acatcttcaa
     1201 gggcacggtc gatgtggtcg tgtgcgacgg tttcgtgggc aatgtggtcc tcaaggcgat
     1261 cgaaggcctg gccaagatgc tctccggcgt gatccgggaa gagttcaagc gcaatttgct
     1321 gaccctgctg gcgggcgcct tgtccattcc cgtgctcaat cgcttgcgca agcgcgtgga
     1381 caaccggcag tacaacggcg cggccctgct gggcttgcgt ggcgtggtca tcaagagcca
     1441 tggctcggcg gatgtctatt cttacggctt tgccctgcag cgcgcgcgcg aggccgtggc
     1501 gagtaaactg ctggagcgca cggcacatgc cgtggcgcaa ataacgaaac gcgagcaggt
     1561 ctcgggcgag gtcgctggcg tgcccgacga ggcaggggac aaggcttgat gagtactccc
     1621 atgatttatt ccgcgatcgc gggcacgggc ggtttcctgc ccgaacgtgt cgtgtccaac
     1681 gatgcgctgg cggccgaact ggccgcgcgc ggcgtcgaaa cctccgacga atggatcgtc
     1741 gagcgcaccg gcatccgcca gcgccatctc gccgcgcgcg gcgtcacgac cagccaactg
     1801 gccaccgagg ccgcccgcca ggccctgcag gacgcgggcg tcgaggcggg cgaggtcgac
     1861 ctgatcatcc tggcgacgtc cacccccgac tacgtgttcc ccagcaccgc ctgcctggtg
     1921 caggccaatc tgggcatcgc gggcggcgcg gcgttcgacg tccaggccgt gtgcagcggt
     1981 ttcgtctatg cgctcacgat cgccgacagc ttcatccgcg tcggccgcgc gcgctgcgcg
     2041 ctggtgatcg gcgccgaagt cttctcgcgc atcctcgact ggaacgaccg caagacctgc
     2101 gtgctgttcg gcgacggcgc cggcgccgtg gtgctcaagg cgtccgacaa gcccggcatc
     2161 atggccgcgc agctgcatgc cgacggcagc ctggccaaga tcctgcacgc cgcgggcaat
     2221 gtgtcccatg gcgaggtcac gggcgatccc ttcctgcgca tggacggcca ggccgtgttc
     2281 aagcaggccg tcaccgtgct ggaacgctcg gcgcgcgccg tgtgcgacga agccggcctg
     2341 ccggtctcgg cggtcgactg gctggtgccg caccaggcca atctgcgcat catcaatttc
     2401 ctgggccgca agctgggcgt gcctgccgaa caagtcgtcg tcacggtcga ccggcatgcc
     2461 aatacctcgg ccgccagcgt gccgctggcg cttgacgccg cgcgccgcga cggccgcatc
     2521 aaggccggcc agacggtgct gatgcagggc gtgggcggcg ggttcacctg gggctcggtg
     2581 ctggtccgcc tgtaagcccg attaaaacga caagacgaaa ctctccatga aaatcgcgtt
     2641 tgtctttccc ggccaaggtt cgcaatcggt gggcatgctc gatgcctggg ccggcaatgc
     2701 cgccgtggcc gatgtgctgg cccgcgccgg cgccgcgctg gagcaggatc tcggcgccct
     2761 gattgcccag ggcccggctg agcagctcaa cctcaccacc aatacccagc ccgtcatgct
     2821 gacggcggcc tacgcctgct atgccgcctg gacggccgcg ggcggccgca agcccgatgt
     2881 catggccggc cacagcctgg gcgagtacac cgccctgacc gccgcgggtt cgctggccct
     2941 cgaagacgcc gtgcgcctgg tgcgcgtgcg cgccgatgcc atgcagaccg ccgtgccggt
     3001 ggggtcgggc ggcatggccg ccatcctggg cctggacgac gacgccgtgc gtgccgtgtg
     3061 cgcccaggcc acggcaagcg gccaacccgg cgaggtggtc gaggccgtca atttcaatgc
     3121 gccggcccag gtggtcatcg cgggctccaa ggccgcggtc gagcgcgcct gcgaggcggc
     3181 caaggccgcc ggcgccaagc gcgcgctgcc gctgccggtt tcggcgcctt ttcactccag
     3241 cctgctcaag cccgccgccg acgtgctggc cgacgcgctg ggcaaggtgc aagtggccgc
     3301 gcctgccatt gcggtcatca ataatgtcga cgtggcgcag gcgggcgacc ccgccggcat
     3361 ccgcgacgcg ctggtgcgcc aggcctggca tcccgtgcgc tgggtcgaaa ccatccaggc
     3421 catgaaggcg cagggcgtca cgcacgtggt cgaatgcggt cccggcaagg tgctggccgg
     3481 cctgaccaag cgcatcgacg gcgacctggt cggcctgacc attaccgatc ccgcctccct
     3541 tgaggccacc cttgccgcgc tggcggcgcc gcaataagca aggacacgga aatgacgacc
     3601 cccacgctca ctacgttcgc tgcccctcga gggggctcca gcctccttcg ggcggccgcg
     3661 cggaggctgg catgagcgcc actgaattgc aaggcaagac cgcgctggtt accggcgcca
     3721 cgcgcggcat cggccgcgcc atcgccctgg aactggccgg ccgcggcgcc accgtcgtcg
     3781 gcacggccac ctccgagtcg ggcgcccagt cggtgcagga ggccctggcc cccctgggcg
     3841 ggcgcggcgc cgtgctgaac gtgaccgatg cggcggcctg cgccgcgctg ctcgacgagc
     3901 tggccaggca gggcggcccg agcatcctgg tcaacaatgc cggcatcacc cgcgacaacc
     3961 tggccatgcg catgaaggac gaagactggg ccgccgtgat cgacaccaat ttgtcggcgg
     4021 tttttcgctt gtcgcgcggc gtgatgcgcg ccatgatgaa ggctcgctgg ggccgcatca
     4081 tcaacgtgac ctcggtggtg ggcgccagcg gcaacgccgg ccaggccaac tacgctgccg
     4141 ccaaggcggg cgtcgcgggc atggcgcgcg cgctggcccg cgaactgggc agccgcaatg
     4201 tcaccgtcaa ctgcgtggcg ccgggtttca tcgataccga catgacgcgc gtgctgggcg
     4261 aagagcaaac cgccgcgctg acccagcaaa ttccgctggg ccgcctgggc gcgccggaag
     4321 acatcgccca tgccgtggcg ttcctggcaa gcccccaggc cggctacatt acgggcacca
     4381 cgctgcatgt taacggcggg atgtatatgt aataacgttg gaacgccact gataaaagta
     4441 at
//
LOCUS       DASWPO010000345         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_975798, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000345 DASWPO010000000
VERSION     DASWPO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975798"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1935)
                     /locus_tag="VGC69_18365"
     CDS             complement(10..1935)
                     /locus_tag="VGC69_18365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0297298.1"
                     /translation="MSISQRLRIIVTILCCLLIALATLLLGYQWRGYHAARQAEDTYQ
                     SFRNTLIVMEDASAERGPTNGALGEDLPIPQARLDGLEKARQATDQALDTLSAQLANS
                     ACYACRSQLLAAKMARGDLNAARKNIDQLLALPRGQRSPEALTDAVNRMVDIIPLFIP
                     IADTIGVEIVKVDGNALDALQMARLASILREQAGLLGSRFTATLTGRRPLLPSEQLSI
                     ERSYGRVEQLYWLIQSRAQDNYAPAREALRRLQDQYMGEGLSYVAHVRAMTAQAPTPA
                     LPSTQQFAERYVPLMRPIVDFRNQMLDLVRAELAENCGRLRTRLAAITAATLVLIALL
                     VLMLWMFRQRVLRPFNEATEEIMSIAAGNISSGPRPHRYQGEIKALFDAMHTLKLHSL
                     DRLHAINYASLIQRAILPDRRMQGSLGKDYFVLWKPRDAVGGDFYLYSDVPQGGLLGL
                     IDCAGHSVPGALMTMLARSAINQATAQSDDQNPAAILMKTDEIMRGMFDAEHFNRSLA
                     TSMDVGLARIDRDARRLRYAGAKMSLYACNGAEVREYKGGRRSIAEKIRCDYVNIDLP
                     LEEGWTYYLCTDGFLDQAGGEQGYGFGNRRFADMLLQLAHLPLSEQAAHADATLTSYQ
                     GERGQRDDITLLAFRIS"
BASE COUNT          336 a          605 c          771 g          385 t
ORIGIN      
        1 ggtcgttatc tagctgatgc gaaatgccag cagggtgatg tcgtcgcgtt ggccgcgctc
       61 gccctggtag gaagtcagtg tcgcgtcggc gtgggcggcc tgttcggaca acggtagatg
      121 ggccagctgc agcagcatgt cggcgaagcg ccggttgccg aagccgtaac cttgttcgcc
      181 gccggcctgg tcgaggaagc cgtcggtgca tagatagtac gtccagcctt cttccagggg
      241 caggtcgatg tttacgtagt cgcaacgaat cttttccgcg atggagcgcc ggccgccctt
      301 gtattcgcgc acttcggcgc cgttgcaggc gtacagcgac atcttggcgc cggcatagcg
      361 cagcctgcgg gcgtcgcggt cgatgcgggc caggcccacg tccatgctgg tggccaggct
      421 gcggttgaag tgctcggcgt cgaacatgcc gcgcatgatc tcgtcggtct tcatcaggat
      481 ggcggccgga ttctggtcgt cggactgcgc ggtggcctgg ttgatggccg agcgcgccag
      541 catggtcatc agcgccccgg gcacgctgtg gccggcgcag tcgatcaggc ccagcaggcc
      601 gccctgggga acgtcgctgt acaggtagaa gtcgccgccc accgcatcgc gcggtttcca
      661 gagcacgaag tagtccttgc ccaggctgcc ctgcatccgg cgatcgggca ggatggcgcg
      721 ctggatgagg ctggcgtagt tgatggcatg caggcggtcc aggctgtgca gcttgagcgt
      781 gtgcatggcg tcgaacagcg ccttgatctc gccttggtag cgatgcgggc gcgggccgct
      841 ggagatattg ccggcggcga tcgacatgat ttcctcggtg gcctcgttga acgggcgcag
      901 gacgcgctgg cggaacatcc agagcatcag cacgagcagg gcgatgagga ccagcgtggc
      961 cgcggtgatc gccgccagcc gcgtgcgcag gcggccgcag ttctcggcca gctcggcgcg
     1021 gaccaggtcg agcatctggt tgcggaagtc gacgatgggc cgcatcaggg gcacatagcg
     1081 ctccgcgaac tgctgggtcg agggcagggc cggggtgggg gcttgcgccg tcatggcgcg
     1141 cacatgggcc acataggaca ggccctcgcc catgtactgg tcttgcaggc ggcgcagggc
     1201 ctcccgggcg ggcgcgtagt tgtcctgggc gcgcgactgg atcagccagt acagctgttc
     1261 gacgcggccg tacgagcgtt cgatggacag ctgctcggag ggcagcagcg ggcgccggcc
     1321 cgtcagcgtg gccgtgaagc gcgaccccag caggccggcc tgctcccgca ggatgctggc
     1381 cagccgcgcc atctgcaggg cgtccagggc gttgccgtcg accttgacga tctccacgcc
     1441 tatcgtgtcg gcgatgggga tgaagagcgg gatgatgtcg accatgcggt tgacggcatc
     1501 ggtcagcgcc tcgggcgaac gctggccgcg cgggagcgcc agcagctggt cgatgttctt
     1561 gcgcgccgca ttcaggtcgc cgcgggccat cttggcggcc aggagctggc tcctgcaggc
     1621 gtagcaggcg ctgttggcga gctgggcgga gagggtgtcg agcgcctggt cggtggcctg
     1681 gcgggccttc tccaggccgt ccaggcgtgc ctggggaatg ggcaggtctt ctcccagggc
     1741 gccgttggtg gggccgcgct cggccgaggc gtcttccatg acgatcagcg tgttgcggaa
     1801 ggactggtag gtgtcctcgg cctggcgtgc cgcgtggtag ccgcgccatt ggtagccgag
     1861 cagcaaggtg gccagcgcga tgagcagaca gcagaggatg gtgacaatga tccggaggcg
     1921 ctggctgatc gacatgaatg ggatggttgt gtatctaaaa attttgttgt gttcgcaatt
     1981 gtctcttatt tcttacgccc gaattattag ggacgtacag atatcgtttt ttgaggcaat
     2041 caatggaacg cagataataa acgccaaaaa aaaggggcca acacaaaaag tgctggc
//
LOCUS       DASWPO010000346         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2227090, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000346 DASWPO010000000
VERSION     DASWPO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227090"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGC69_18370"
     CDS             <1..267
                     /locus_tag="VGC69_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297299.1"
                     /translation="RRFLQDPETALGAAAMQTLRDIGARLDLDYAGIDFSLLPDGRVL
                     VFEANPTMLVHPEKPGGPYAHKNAYVRRILHAFADMLERRIIAG"
     gene            complement(269..2731)
                     /locus_tag="VGC69_18375"
     CDS             complement(269..2731)
                     /locus_tag="VGC69_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEY0297300.1"
                     /translation="MEPPIFGRLFLLAFVAGIGLAHLWADLPALPRLYAACAIGMLLC
                     LLAVRLRAYRLRAACLIAAGLLAGLADTGLQAHGRLSEMLADEHHNQVTRLRVQAIAL
                     SQDDESGRRFLAEAVGERPAGIPRRIRVTWHGRPGAQDTLPEVVPGQVWRMALILRRP
                     YGPANPHAMDLELRDFAEGVRAEGTVRGKPVLLDDQPWAGAGIAIERLRHRIRAGMRQ
                     ALGGQRYGPVLIALAIGDQAGVAREDWQVFNRSGISHLVSISGSHVTLVAAMGGLLMA
                     GLWRRARWRGVGVAQYLPAQLAGGWAALLVALGYCLLAGWGVPARRTFFMLACLAMAG
                     LARLPLGGSRLLALAGACVLALDPWGMLSPGFWLSFGAVAILMRIASAHEQPAQDRRE
                     RVRRVVAGFCKTQGAITLGLTPLLAFLLHQVSLGSPLANAIAVPVVGAVVTPLALLCG
                     ALSVAPGGQGLAWAAGWLGHGLFAAVMQPVGWVGRADWAAFSVAAAPWPWLLPALAGL
                     VFALQGKGGRWRLLGWLGMLPLLLWRPDRPPPGDWRLTALDVGQGSALLIETATRTLL
                     YDVGPRYYGGGDAGERVLAPYLRARGIRQVDALVVSHADLDHAGGLRSILQAFPVSVS
                     YASFDLAAHVRREAAHLSEADLGDAQWPSSQRPCEAGMQWESDGVLFRFIHPPPDAGR
                     LGGKGDSNARSCVLLVQGRRHAILLPGDAGLAQEARYAAALPPLAVVAAPHHGSSTSS
                     GQALVRAAGPTQVIVQAGYLNRFRHPSPDVVSRWQSAGASVWRTDLQGALTVESSARG
                     LAVHAQRTDARRYWHADLPWTP"
     gene            complement(2782..3618)
                     /locus_tag="VGC69_18380"
     CDS             complement(2782..3618)
                     /locus_tag="VGC69_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY0297301.1"
                     /translation="MPKFAANITMMFTEMPLLDRIDAAARMGFEAIECSQPYEADAGE
                     FAARARSAGIAVALLNFPAGDWSAGERGLACHPGRLADFRASVEAGISFAKQCGASRM
                     NCLAGVVPAGIDDAAARETLVSNLRHAAAELGNAGMDLLVEPINRYDLPGYAVAGSSG
                     AIEVLDAVGAPNAFLQYDVYHMQRMEGELAATLQRLIGRIGHVQIADTPGRHEPGTGE
                     INYRFLLPWLDHIGYQGWVGCEYVPKAGTEKGLAWREEHGVRGARQGSTESRDTVRGC
                     DV"
     gene            complement(3638..4066)
                     /locus_tag="VGC69_18385"
     CDS             complement(3638..4066)
                     /locus_tag="VGC69_18385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0297302.1"
                     /translation="MPVQRIDHINLSTSDLDRTRRFYKEILGLEDGPRPPFQRKGAWM
                     YLDGRPVIHLSTGRTPLTRSSDSFDHVAFYCRELSVFRERLRLHALHYEAYRVPAQNM
                     YQLFFRDPDGTEIELIFDLSEDPSPPDAETGRDATMGRTT"
     gene            complement(4081..>5123)
                     /locus_tag="VGC69_18390"
     CDS             complement(4081..>5123)
                     /locus_tag="VGC69_18390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297303.1"
                     /translation="RKAGDKIMKSAKRCALCTGGAAIALVQGLLLWFASQGAAMAQDA
                     SNDASYPDHPIRMIVPYSAGSASDVLARVTGDRLSQVMGQSVIVDIKPGASGVIGAQV
                     AAAAPHDGYTFVLLNDATAALYPALLPTKPYDMRKDFSPLSYAGDVRMVLIVNASTPV
                     KNVADLIALAKEKNGHLDYASGGYGSVQQVLMEIFMDAAGVKLSHVPYKGVVPAVTDV
                     VAGRVPVIFASLTSVLPYIKSGSVRALAVTSEHRVEALPDVPTMTEAGIAGFPSEPWN
                     AFFAPANVPAPVLARLSKALTEVLREPGVQAKLENIIAVRAGPPEELKQVLDRDIDRY
                     GSLVKKVGMKID"
BASE COUNT          918 a         1807 c         1632 g          766 t
ORIGIN      
        1 cggcgcttcc tgcaagaccc cgaaaccgcg ctcggcgccg cggccatgca aacgctgcgc
       61 gacatcggcg cgcgcctcga cctggattac gcgggcatcg atttttcctt gctgccggac
      121 gggcgcgtac tggttttcga ggccaacccg accatgctgg tccatccgga aaagcccggc
      181 ggcccctacg cgcacaaaaa cgcctatgtg cggcgcattc tccatgcctt cgcggacatg
      241 ctggaacgcc ggatcatcgc ggggtagatc acggcgtcca cggcaggtcg gcgtgccagt
      301 agcgccgggc gtccgtgcgt tgcgcatgca cggccagccc ccgcgccgac gactccaccg
      361 tcaaggcgcc ttgcaagtcc gtccgccata cggacgcgcc ggccgactgc cagcgcgaca
      421 ccacgtccgg cgagggatgg cgaaagcggt tcaggtagcc ggcctggacg atcacctgcg
      481 tcggccccgc ggcccgcacc agggcctggc ccgacgaggt ggatgaacca tggtgcggcg
      541 ccgccacgac agccagcggc ggcaatgccg cggcatagcg ggcctcctgc gccaggccgg
      601 catcgccggg cagcaggatg gcgtggcgcc ggccctgcac cagcagcacg cagctgcggg
      661 cattgctgtc gcccttgccg cccaggcggc ctgcatccgg tggcggatgg atgaaacgaa
      721 acagcacgcc gtcgctttcc cattgcatgc cggcttcgca aggccgctgg ctcgacggcc
      781 actgcgcgtc gcccaggtca gcctcggaca aatgggccgc ctcgcgcctg acgtgcgccg
      841 ccaggtcgaa cgaggcatag gacacactga ccggaaacgc ctgcaggatg ctgcgcagcc
      901 cgccggcgtg gtccaggtcg gcatgcgaca ccaccagcgc atccacctgg cgaattccgc
      961 gtgcgcgcag gtacggcgcc agcacccgct cgccggcatc gccgccgccg tagtagcgcg
     1021 gccccacatc gtaaagcagc gtgcgggtgg ccgtttcgat gagcagcgcg ctgccctggc
     1081 ccacgtccag cgcggttaat cgccagtcgc cgggcggcgg cctgtcgggc cgccacagca
     1141 acagcggcaa catgcccagc caccccagca ggcgccagcg gccgcccttg ccctgcaagg
     1201 caaagaccag gccggccaag gccggcagca gccagggcca gggcgccgcc gccacgctga
     1261 aagccgccca gtcggcccgg cccacccagc ccaccggctg catcacggcc gcgaacaagc
     1321 catgccccag ccagcccgcg gcccaggcca ggccctgccc gcccggcgca acggacaagg
     1381 cgccgcacag caaggccagg ggcgtcacca ccgccccgac gacaggcacg gcaatggcat
     1441 tggccagcgg cgaacccagc gacacctggt gcagcagaaa agccagcaaa ggcgtcaacc
     1501 ccagcgtgat cgcgccttgc gtcttgcaaa agccggccac gacgcgccgc acccgttcgc
     1561 ggcgatcctg ggcaggctgc tcatgcgcgg aggcgatgcg catcaaaatg gccacggcgc
     1621 cgaaagacag ccagaacccc ggcgacagca tgccccaggg gtccagcgcc agcacgcagg
     1681 caccggccag cgccaggagg cgcgaaccgc ccaagggcag ccgcgccagc cccgccatgg
     1741 ccaggcaggc cagcatgaaa aacgtacgcc gggccggcac gccccagccc gccagcaggc
     1801 aatagcccaa ggcgaccagc agggccgccc agccgcccgc cagctgcgcg ggcaggtatt
     1861 gcgccacgcc caccccgcgc cagcgggcgc gccgccacaa gccggccatg agcagcccgc
     1921 ccatggccgc caccagcgtg acgtgcgatc cgctgatcga gaccaggtgc gagatgccgc
     1981 tgcgattgaa gacctgccag tcctcccgcg ccacgccggc ctggtcgccg atggcgagcg
     2041 cgatcagcac cggcccatag cgctgcccgc ccaacgcttg ccgcatgccg gcgcggatgc
     2101 ggtggcgcag gcgctcgatg gcgatgccgg cgccggccca gggctggtcg tccagcagca
     2161 cgggcttgcc gcgcaccgtg ccttcggccc gcacgccttc ggcgaaatcg cgcagttcca
     2221 ggtccatggc atgcggattg gccgggccgt agggccggcg caggatcagg gccatgcgcc
     2281 agacctggcc gggcacgacc tcgggcaagg tgtcctgcgc gcccggccgg ccatgccagg
     2341 tcacgcggat gcggcgcggt atgcctgccg gccgttcgcc gacggcctcg gccagaaagc
     2401 ggcgcccgct ctcgtcgtcc tgcgacaacg cgatggcttg cacccgcagt cgcgtcacct
     2461 gattgtggtg ttcgtcggcc agcatctcgg acagccggcc atgcgcctgc aagccggtat
     2521 cggccaggcc ggccaacagg cccgcagcga tcaagcatgc agcgcgcagt cgataggcac
     2581 gcagtcgcac agccaacaga cacagcagca tgccaatggc gcaggctgcg tacagccgcg
     2641 gcaaagcagg caggtcggcc cacaggtgtg cgagtccgat accagcgacg aaagccagca
     2701 ggaataaacg gccgaagatg ggaggctcca tagaagcctc agcttagcga acctcccggc
     2761 ggcgtgcgcc ggcacaatga tctatacgtc acaccctcgc actgtgtcgc gtgactcggt
     2821 tgatccctga cgcgcgccgc gcacgccatg ctcctcacgc caggcgagcc ccttttcggt
     2881 tcctgccttg ggcacgtatt cgcagcccac ccagccctgg tatccgatgt gatcgagcca
     2941 tggcaataga aaccggtagt tgatttcgcc ggtgcccggt tcgtgccggc ctggcgtgtc
     3001 ggcgatctgc acatgcccga tacgccctat cagccgctgc agcgtggccg cgagctcgcc
     3061 ttccatgcgc tgcatgtgat aaacgtcata ctggagaaac gcattggggg cgcccaccgc
     3121 atcgagaacc tcgatggcgc cgctcgatcc ggccacggcg tacccgggca ggtcatagcg
     3181 gttgatgggt tcgaccagca aatccatgcc ggcattgccg agctcggccg cggcatggcg
     3241 cagattgctc accagcgttt cgcgcgccgc ggcatcgtct atgccggcgg gcaccacgcc
     3301 cgccaggcaa ttcatgcggg acgcgccgca ctgttttgca aaggaaatgc ctgcctcgac
     3361 gctcgcgcga aagtccgcga ggcgcccggg gtgacaggca aggccccgct caccggcgct
     3421 ccagtcacct gccgggaaat tgagcagcgc caccgcaata cctgcggatc gcgcgcgcgc
     3481 ggcgaattcc ccggcgtcgg cctcgtaggg ctgggaacat tcgattgctt cgaagcccat
     3541 tcgggccgcg gcatcgatgc gatccagcaa cggcatttcg gtgaacatca tcgtgatatt
     3601 ggcggcgaat ttcggcatga ctcatcccta tgcgtgatca ggtcgtgcga cccatggtgg
     3661 catcgcgccc cgtttcggcg tccggcggtg acggatcttc cgacagatcg aagatcaatt
     3721 cgatttccgt gccgtccgga tcgcggaaaa atagctggta catgttctgc gcgggaaccc
     3781 ggtacgcttc gtaatgaagc gcatgcaggc gcaatcgctc ccgaaagacg gacaactcgc
     3841 gacagtaaaa tgccacgtga tcgaaggaat cggagctgcg cgtcagcggc gtccggccgg
     3901 tggaaagatg gatgacggga cgtccgtcca ggtacatcca cgcccccttg cgctggaatg
     3961 gcggacgcgg gccgtcttcc aggccgagaa tttctttgta gaaacgtcgg gtccgatcca
     4021 ggtccgaggt gctcaagttg atgtggtcga tgcgttgaac cggcatgatt tttcttcgcc
     4081 tcaatcgatt ttcattccta ctttcttgac caggctgccg tagcgatcga tgtcgcggtc
     4141 gagcacctgc ttcagctctt ccggcggacc cgcgcgcaca gcgatgatgt tttcaagctt
     4201 ggcctggacc ccgggctccc taagcacctc ggtcaacgcc tttgaaagac gcgccagcac
     4261 cggagcgggc acgttggcgg gcgcaaagaa ggcattccac ggctcgctgg ggaaacccgc
     4321 gatgcccgct tccgtcatcg tgggcacatc cggaagcgct tcgacccgat gttcgctggt
     4381 gaccgccaag gcacgcacgc tgcctgattt gatatagggc aggacgctgg tcaggcttgc
     4441 gaagatcacc gggacccgac ccgccaccac gtccgtcacc gccgggacga cgcctttata
     4501 cggaacgtgg gacagcttca cgccggcggc atccatgaat atttccatca atacctgttg
     4561 cacgctcccg tagccacccg acgcatagtc cagatggccg tttttctctt tcgccaacgc
     4621 gatgagatcc gccacgttct tgactggcgt ggacgcattg acgatcaaca ccatacgcac
     4681 gtctcccgcg tagctcaacg gcgagaagtc cttgcgcatg tcgtatggct tcgtgggcag
     4741 caatgcgggg tagagtgcgg cagtcgcgtc gttgagcagc acgaaggtgt agccatcgtg
     4801 cggcgccgcg gccgcgacct gtgcgccgat gaccccgctt gcgccaggct tgatgtcgac
     4861 gatgaccgat tgccccatca cctgggacag gcgatctccg gtgacacgtg ccagcacgtc
     4921 cgacgcgctg cccgcggaat agggaacgat cattcgtatc ggatgatcgg gataggacgc
     4981 atcgttcgat gcgtcctggg ccattgccgc gccctggctg gcaaaccaga gcagcagccc
     5041 ctgcacgagg gcgatggccg cgccgcccgt gcacaacgcg caacgcttcg cgcttttcat
     5101 gattttgtct cctgcttttc tgt
//
LOCUS       DASWPO010000347        21068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1870946, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000347 DASWPO010000000
VERSION     DASWPO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 21068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21068
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870946"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGC69_18395"
     CDS             complement(<1..263)
                     /locus_tag="VGC69_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0297304.1"
                     /translation="MPLSTYRDDPQAIKDLVRETEVHKDLFVSPELYELEMERLFAVT
                     WVYVGHESQVPKPGDFYTTTVGDQSVVMVRHGDQSIRVLYNRCP"
     gene            complement(286..1248)
                     /locus_tag="VGC69_18400"
     CDS             complement(286..1248)
                     /locus_tag="VGC69_18400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY0297305.1"
                     /translation="MQTLALVVREVRQESPLIRAFRLAAADGGALPPYTPGSHLKVQV
                     PGLHDPRCYSLINTGGEAFAAPGEYRLGVRIEENSQGGSRYMHALKEGDTITAQGPAN
                     EFPLADHPGKTVLIAGGIGITPMASMAASLAAKGNDYVLHYSGRSRGQLAFLGELQAL
                     AGDKLAVHADDEPASAFDLDALLDAAPAGQHVYVCGPKGLIDAVIAGARKRDWDGKRV
                     HFELFTAAAPQAGDGAFEVELKQSGRVLQVPADKTIIDVLEAAGCDPMYDCKRGECGV
                     CQATVLEGTPDHRDYYLSDAEKAKGNVIQMCISRAKSARLVLDL"
     gene            complement(1275..2840)
                     /locus_tag="VGC69_18405"
     CDS             complement(1275..2840)
                     /locus_tag="VGC69_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEY0297306.1"
                     /translation="MDSAANLIPSNPIKIAILAMGGQGGGVLADWIVEMAEHAGWWAQ
                     TTSVPGVAQRTGATIYYLEIVPAADIKRAGHAPTLALMPTPGDVDLIVAAELMEAGRA
                     MQRGLVTPDRSVLITSSHRSYAIDEKTTPGNGIADPNQVLDVGREAARRFICFDLQAA
                     AEKAGSVISASLFGAIAGSQALPFPREEFEATIRRAGVGVEASLRAFALGFDGAAHAP
                     AQPAPIDLSRPEPEVPVSAKDKRVQALLDRVHTGFPAVAQPMLVAGLRRLIDYQDLAY
                     AHDYLEHVAAIRQLDVQYGGEKACWALTCSAARYVAVAMSYDDVIRVADLKTRGARFD
                     RVRKEVGVKEGQLLYTTEFMHPRLEEICGTLPAGLGRWLEKSALGKFVERRLGKGKRL
                     KSGTLSGFFSLYLMAGMRRWRRSTLRHQAEAASLKQWLDLIARIAPRDYALAVEVVEC
                     RRLVKGYSDTHVRGGGKYRTLLQAAEGLLGRPDAALAMRELRATALTEVQCAPLERQA
                     TEKLLPPQAAPAA"
     gene            complement(2853..5072)
                     /locus_tag="VGC69_18410"
     CDS             complement(2853..5072)
                     /locus_tag="VGC69_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEY0297307.1"
                     /translation="MAERSFKKEVQQLRIGAGEEFRGEGILAITKALLQSGVGYVGGY
                     QGSPISHLIDVLTDASDILEELGVHLETSASEATAAATLAASVMYPIRGAVAWKSTVG
                     TNVASDALANLASGGVTGGAVVIVGEDYGEGSSIMQERTHAFAMKSQMWLLDPRPNLE
                     TMVKSVEDSFELSEASNTPVMMQVRVRACHVHGRFIAKDNKRPAHTLAEAMESPRRDV
                     NRIVLPPAAYVHEHEKIKQRWPAAVKFIKERKLNEFFEGDIDDIGLIVQGGVYNNAIR
                     ALQLLGLADHFGNSRIPLYVLNVVYPIIDDEVIGFCKDKKAVLLLEEGQPDYIEQNLH
                     TILRRADIPTPLWGKNVLPLAGEYTTAVLRSGVEAFLKAWKLEALHAHRAVAADEQAA
                     KAANQLEITEVSRPRPVEQPITLHKRIAGLSKVVPQRPAGLCTGCPERPIFSAITLAQ
                     DKLGEHHISCDIGCHLFSILPPFNLGATTMGYGLGAASSSAFNIKGAKRSISIMGDGG
                     FWHNGLTSGIGNAVFNKFDGVIVIVDNFYSSATGGQDILSSRAQNAERATNNPIEAAV
                     RGVGVKWLRVIDHTYDVTKARNLLEEALTTDAEGPKVIIAQSECMLNKQRRVKPAFNK
                     AVKEGKRVVKERFGVDADVCSGDHACIRLSGCPSLSIKDSGDPLKVDPVASVDSSCVG
                     CGNCGEVAEAAVLCPSFYRADVVHNPTGWDRFAGRVRGAVIGYLQRRRETRLARYAL"
     gene            5388..5882
                     /locus_tag="VGC69_18415"
     CDS             5388..5882
                     /locus_tag="VGC69_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0297308.1"
                     /translation="MKSDKQQDTGPASSFINGYLAYLLAQVSHSISAEFHAQAHEAGL
                     SVTEWRVLSSLMGGSGLTIGELAFLAITKQPTLSKVVQRMEANGLVARAEVRADRRQT
                     RVSLTARGTRLAETLCQQAMRHQMAVLEPFGEARSAELMEMLRTLIVLHVPLPARGED
                     EAAD"
     gene            complement(5934..7511)
                     /locus_tag="VGC69_18420"
     CDS             complement(5934..7511)
                     /locus_tag="VGC69_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297309.1"
                     /translation="MRAALAGLFVFAIAPAFAKPLTVCTESSPDGFDVVQFNSLVTSN
                     ASADVVFDQLVTYDEAAKKVSPSLAERWEISDDGLAYTFHLRSNVQFQTTAWFKPTRA
                     FGADDVMFTFNRMLDPNDPWYKATPGGFPHAQSMGLSKLIKSVSKVDDHTVRFELTEP
                     NATFLSILTMGFASIYSAEYANQLLQAGKPADLNSQPVGTGPFSLRSYTKDAAIRYAV
                     NPSYWGGKPKIDALIYAITPDANVRAQKVKAGECQIALSPKPQDIAAAKGDKALKVEE
                     TPAFMTAFVALNTQKKPLDDAKVRQALNLAFDRASYLKAVFDDTATAAINPWPSSAWG
                     YNRSIQPYPYDPARARQLLAQAGYPNGFSTTIWVRPTGSVLNPNPGVGAQLLQADFAK
                     IGVRAEIKTIEWGQLIREAKQGQHDTLFMGWAGDNGDPDNYLTSLFSCNAVQSGTNFS
                     RYCDPQLDALIAQGKSTSDPAKRTQAYEAAQQIIHDQALWIPLGYPVAAVIVRATVSG
                     YQVSPFGRQDFTHVSVQ"
     gene            complement(7669..8154)
                     /locus_tag="VGC69_18425"
     CDS             complement(7669..8154)
                     /locus_tag="VGC69_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297310.1"
                     /translation="MPTPPSSPAAAPRFTQAELDMLAKTADALTAYLGRPVLAEVGVD
                     EDGVEWVTFGSTLDEKDKSAGDEEHLHVQLGGKDARLLGQSGGLPESADTYDCRYLLA
                     IQLSATEGERFVKLDGDGEECAWSDELAEVLPFSLEEEELPPLDDDEDEDDDDGAPPS
                     H"
     gene            complement(8207..9421)
                     /locus_tag="VGC69_18430"
     CDS             complement(8207..9421)
                     /locus_tag="VGC69_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297311.1"
                     /translation="MKPMFRSLRIRNYRLWAGCALISNIGSWMQRTAQDWLVLTDLTP
                     HNARAVGITMALQFGPQVLLIGLTGYAADHFDRRRLLLCTQGAMGALALGLGLLALSG
                     AAQLWQVYGFALALGCAAAFDMPARQTFVAELVGEEDLPNAVALNSTSFNLARMAGPA
                     LAGLLIAAMGTGWVFLLNACSFAAVIFALLRLRASELHSKERAARQRGNLAAGLRYVW
                     QREDLRAIMITLFLIGAFGLNFPIFIATMAVSVFHADAGRYGLLTTMMAVGSVCGALL
                     AAGRDKPGLHWLYLGVAVFGLGCATAALMPNYWLFGAALVIIGIAAQTVTTSANSLVQ
                     LSTEPAMRGRVIAILLAVALGSTPIGAPLVGWIANRFGPRWALGMAALSGFGGALVGL
                     RYLIRQRRPALQ"
     gene            complement(9427..9993)
                     /locus_tag="VGC69_18435"
                     /pseudo
     CDS             complement(9427..9993)
                     /locus_tag="VGC69_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            complement(10028..10330)
                     /locus_tag="VGC69_18440"
     CDS             complement(10028..10330)
                     /locus_tag="VGC69_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297312.1"
                     /translation="MHPISLFITGMLFCNSVPHLVCGLQGQRFPTPFAKPRGIGLSSP
                     LVNFVWGFANLLVALALYARHPAALDANADTLAMLAGVAVLGVFSAVHFGKVRNAA"
     gene            10516..11130
                     /locus_tag="VGC69_18445"
     CDS             10516..11130
                     /locus_tag="VGC69_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0297313.1"
                     /translation="MATATSDSRAAGVRAPKRERGRRRVEAILAAAMEVFAEKGYDAA
                     TMTEIAARSDTAIGSLYRFFASKEVLADALLLRYAQHVGQGLEEIGRQVPGLSAESVA
                     GLLVDFMVALQAQRRFAVVLVDARGGSGNRRALFRRTIRAGLAEILLKVSDKLTPARA
                     QTMAPVLQHLLKGVALAADEEPAVQAALTGEIRELARLYLLSVR"
     gene            complement(11164..11985)
                     /locus_tag="VGC69_18450"
     CDS             complement(11164..11985)
                     /locus_tag="VGC69_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY0297314.1"
                     /translation="MLDYEFMRNAYAAAGIVAVVAAMVGYFLVLRGQSFAGHALSHIG
                     FSGATGAALVGLAPLNGMLIAALAGGVCIGLLGERLAQRDVAIGIALSLSLGLGLLFL
                     HFYTAYATQASTLLFGNVLAVSGETVRALLVLGLACLAGLAAISRPLLFASLQPELAQ
                     ARGVSLRTYSALFMAIAALATVLSTQVVGILLVFSLMVGPPATAQRLTRRVGSGLALS
                     AALALACAWLGIALAYWTDWPCSFWITALSCAAYLAAQAWAWLRGRSAPRSAARP"
     gene            complement(12034..12834)
                     /locus_tag="VGC69_18455"
     CDS             complement(12034..12834)
                     /locus_tag="VGC69_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0297315.1"
                     /translation="MPAIELDRVRLALGGRTVLDDVSLAIEQREFIGVLGANGAGKTT
                     LMRAVLGLVEPAAGRIRVLGDAARRGNPAIGYLPQTRAIAPAVRLSGRHVVASALNGH
                     RLGLAWQGKAARREVDRVLDLTGARDLAERPIAELSGGERQRLLLAQALLGRPELLLL
                     DEPLVSLDPRRQQAMVDLVKALQAELGITVLLSAHELHPLLGALDRVLYLGQRHAALG
                     TVEEVVTGPVLSRLYGTRIDVVRAAGRLFVIAGGVDAETDAHRHEAHA"
     gene            complement(12834..13706)
                     /locus_tag="VGC69_18460"
     CDS             complement(12834..13706)
                     /locus_tag="VGC69_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY0297316.1"
                     /translation="MRRILPFVRFLACLSLLPAGAWAAPVPIVAAENFYGDVARQIGG
                     DDVSVISILQSPDQDPHLFEASPSVAREIAAARLVVQSGIGYDGWMEKLLQAGHDAGP
                     QVLTVAPLAGRQDGDNPHIWYDPQTMLAYAAVLTERLASADPEHAAGFRERLARFQAS
                     MHPVLAKIDALRASYAGTPVAATEPVFGYLIDAAGLLNRHAAFQLAVMNDTEPSASDV
                     AEFERSLQTHAVKLLVYNSQATDPLADRMQRLAERAGVPVLPVTETEPEGVAYQDWLL
                     GILDRLQQALSKSK"
     gene            13897..15570
                     /gene="ettA"
                     /locus_tag="VGC69_18465"
     CDS             13897..15570
                     /gene="ettA"
                     /locus_tag="VGC69_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY0297317.1"
                     /translation="MAQYVYTMNRVGKIVPPKRQILRDISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGVDKEIEGEAIPMPGLNIGYLPQEPQLDPGHTVRQAVEEGLGAAMAAKK
                     RLDEVYAAYAEPDADFDALAAEQADLEAIIAAAASSGADDIEHQMEIAADALRLPPWD
                     AIVGNLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLHKFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKDARLKQEESSESARQKTIKKEL
                     EWVRQNPKGRQAKAKARIARFEELSSYEYQKRNETQEIFIPVGERLGNEAIEFNGVSK
                     AYGDRLLIDNLSFKVPPGAIIGIIGPNGAGKSTLFRMIAGKEQPDSGEVAIGQTVKLA
                     YVDQSRDALEDKKTVFDAISDGADLLTVGKFEMSSRAYLGRFNFKGSDQSKIVGQLSG
                     GERGRLHMAKTLIAGGNVLLLDEPSNDLDVETLRALEDALLEFPGSVMVISHDRWFLD
                     RIATHILAFEGDSQVVFFDGNYQEYEADKVRRLGAEGAKPKRLRYKALK"
     gene            15787..16011
                     /locus_tag="VGC69_18470"
     CDS             15787..16011
                     /locus_tag="VGC69_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY0297318.1"
                     /translation="MNIRTLSQVSLAALLAISMAGCSSWDHMSRRSKTMVGGAALGGL
                     AGAVITGGGVLGTVGGAAIGGVVGDQVGKR"
     gene            16220..17350
                     /locus_tag="VGC69_18475"
     CDS             16220..17350
                     /locus_tag="VGC69_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297319.1"
                     /translation="MLLLTAGCVLATGQAFAAAKPVRIGVTTILSGPYADRGQSEQYG
                     VELALQQINQAGGVLGRPVEAFYADNADDTAKGLAAAKRLIEQQRVPVLIGALATPVT
                     RAIMPVANAAKVPLVIDISAGHEFVDAAGSGGYSYVFKTSPSDVDVAAGMMQWLKGKR
                     VGSVAIVADDNAFNLANAASMAQAAAAAGIKVLSSDVVAKGTTDLSGLLGKIGAAHPD
                     RIVTVLGPSSGLFFKAYEQDAAHIPLAGRIDFSAVKASLSPQFLAAGGLDQAAGVSVY
                     TPALDTPAVRAFAMAYEDKYGLAPTQRSLLGFEAARLVVDAIRRAKSDDPAAIERALK
                     STKMPSLLGGTFAMDAHNHAHTGMQILGWRGNGVAVLERIGA"
     gene            complement(17365..18123)
                     /locus_tag="VGC69_18480"
     CDS             complement(17365..18123)
                     /locus_tag="VGC69_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0297320.1"
                     /translation="MTRKILLITGASRGLGKSTLLNAARHGIDAVFTYNSNKAEADKV
                     VAEAAALGARAVALQLNVEDVAAFAAFAEQVKAALARHWQREQFDYLVNNAGTGIHAS
                     VADTTEAQFDTMMRQHLKAPFFLIQALLPLIADGGRIVNLSSGLARFSLPGFAAYASM
                     KGGVEVLTRYLALELGPRGIAVNTVAPGAIATDFGGGVVRDNAQMNAAIAKQTPLGRV
                     GLPDDIGGAIAMLLADENRWINAQRIEVSGGIHY"
     gene            18292..19212
                     /locus_tag="VGC69_18485"
     CDS             18292..19212
                     /locus_tag="VGC69_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0297321.1"
                     /translation="MNRYDAMRLFVRAAETSNFTQAADSLGVPKASMSNAVRQMETQL
                     GVRLFTRTTRRVALTQEGRMCLDRCKDILADIDDLESLFSTDKTVSGLLRVDMPHALA
                     CALVIPRLPDFFDQHPGLELELSTTDRRVDLLREGFDCVLRVGAVQESSLIARPLGEL
                     PQINCASPRYLARFGEPRTLEDLRGHRLVMYVPEPGMRDPGWQYHDGERYAHVPMGGV
                     LAVNGVDAYQEACLAGLGMIQVPAYGVQAYLDSGRLKEVMPAHQAAPLPVTLLYADRR
                     HAPLRVRVFMQWLAELVAGAYPGVPHAGRP"
     gene            complement(19223..19483)
                     /gene="minE"
                     /locus_tag="VGC69_18490"
     CDS             complement(19223..19483)
                     /gene="minE"
                     /locus_tag="VGC69_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEY0297322.1"
                     /translation="MSFLSFLIGEKKKTATVAKERLQIILAHERSGRNASPDYLPQLQ
                     KELVAVISKYVKINPEDIKVHLERQDTLEVLEVKIEMPQPEA"
     gene            complement(19487..20302)
                     /gene="minD"
                     /locus_tag="VGC69_18495"
     CDS             complement(19487..20302)
                     /gene="minD"
                     /locus_tag="VGC69_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEY0297323.1"
                     /translation="MARIVVVTSGKGGVGKTTTSASFSSGLAMRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDFVNVIQGEASLKQALIKDKQLENLFVLPASQTRDKDALTQEGV
                     GKVMDDLKEMGFDYIVCDSPAGIETGALMAAYFADDALVVTNPEVSSVRDSDRILGIL
                     AAKSRRAIESDEPVKEYLLLTRYNPKRVSDGEMLSLTDIEDILRIKLIGVIPESESVL
                     AASNQGLPAIHLQGTDVSEAYQDVVARYLGEERALRFTDYSKPGLLKRIFGGK"
     gene            complement(20420..>21068)
                     /gene="minC"
                     /locus_tag="VGC69_18500"
     CDS             complement(20420..>21068)
                     /gene="minC"
                     /locus_tag="VGC69_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEY0297324.1"
                     /translation="VVIDASRVEDAIDWTHLLAALRRYNLPAIGVVAEGGNLEAARQA
                     GLVPVDLSTATPRAAAAMPAAAPMAAQAAEPADPAEPPVAAPTPAVPGIPVAADTPPA
                     PSSAAMIVNRPLRSGQRVYARNTDLVVIGMVSQGAEVIADGNVHVYGPLRGKAMAGAR
                     GDTSARIFTTQLDPELLAVAGVYRVVEDKLDQRVHGKPALVSLDGETLKIEAIGE"
BASE COUNT         3337 a         6662 c         7566 g         3503 t
ORIGIN      
        1 gggcagcggt tgtacagcac gcggatgctc tggtcgccgt ggcgcaccat caccacggac
       61 tggtcgccca cggtggtggt gtagaaatcg ccgggcttgg gcacctggct ttcgtggccc
      121 acgtaaaccc aggtcacggc gaagaggcgt tccatttcga gctcgtagag ctccgggctg
      181 acgaagaggt ccttgtggac ctcggtttcg cgcaccaggt ccttgatggc ctgggggtcg
      241 tcgcggtagg tggatagtgg catgggaggc tcccgcaatc gggtcttaca ggtccagcac
      301 caggcgggcc gacttggcgc gcgagatgca catctggatg acattgccct tggccttctc
      361 ggcgtcggac aggtaatagt cgcggtggtc gggcgtgcct tcgagcacgg tggcctggca
      421 gaccccgcac tcgccgcgct tgcagtcgta catgggatcg cagccggctg cttcgagcac
      481 gtcgatgatg gtcttgtcgg cgggcacttg cagcacccgg ccggactgct tgagctcgac
      541 ctcgaaagcg ccgtcgccgg cctgcggcgc ggcggcggtg aagagctcga agtgcacgcg
      601 cttgccgtcc cagtcgcgtt tcctggcgcc ggcgatgacg gcgtcgatca ggcccttggg
      661 gccgcacacg tagacgtgct ggccggcggg cgcggcgtcg agcagggcgt cgagatcgaa
      721 ggcgctggcg ggctcgtcgt cggcgtggac ggccagcttg tcgccggcca gggcctgcag
      781 ctcgccgaga aaggcgagct ggccgcggct gcggccgctg tagtgcagga cgtagtcatt
      841 gcccttggcg gccagcgagg cggccatgct ggccatgggc gtgatgccga tgccgccggc
      901 gatcagcacg gtcttgcccg ggtggtcggc cagggggaat tcgttggccg ggccctgcgc
      961 ggtgatcgtg tcgccttcct tgagcgcgtg catgtagcgc gaaccgccct ggctgttttc
     1021 ttcgatgcgc acgcccaggc ggtattcccc gggcgcggcg aaggcctcgc cgccggtgtt
     1081 gatcagcgag tagcagcggg gatcgtgcag gccgggcact tgcaccttga ggtgcgagcc
     1141 cggggtgtag ggcggcaggg cgccgccatc ggcggcggcc agccgaaagg cgcggatcag
     1201 gggcgattcc tggcggactt cacggacgac gagggcgagg gtctgcatgg cggtcggttc
     1261 cggatggctg gcgctcaggc cgccggggcg gcctgaggcg gcagcagttt ttcggtggcc
     1321 tggcgctcca gcggggcgca ctgtacttcg gtcagggccg tggcgcgcag ttcgcgcatg
     1381 gccagggcgg catcgggccg gcccagcagg ccttcggcgg cctggagcag ggtgcggtat
     1441 ttgccgccgc cgcgcacatg ggtgtcgctg tagcccttga ccaggcggcg gcattccacg
     1501 acttcgacgg ccagcgcgta gtcgcgcggc gcgatcctgg cgatgaggtc cagccattgc
     1561 ttgaggctgg ccgcttcggc ctggtggcgc agcgtgctgc ggcgccagcg gcgcatgccg
     1621 gccatcaggt atagcgagaa gaagccggag agcgtgccgc tcttcaggcg cttgcccttg
     1681 cccagccggc gttcgacgaa tttgcccagc gccgattttt ccagccagcg gcccaggccg
     1741 gcgggcagcg tgccgcagat ctcttccagg cgcgggtgca tgaattcggt ggtgtagagc
     1801 agttggcctt ccttgacgcc gacttccttg cgcacgcggt cgaagcgcgc gccgcgggtc
     1861 ttgaggtcgg ccacgcggat cacgtcgtcg tagctcatgg ccacggccac atagcgcgcg
     1921 gcggagcagg tcagcgccca gcacgccttt tcgccgccgt actgcacgtc gagctggcgg
     1981 atggcggcca cgtgttccag gtagtcatgg gcgtaggcca ggtcctggta gtcgatcagg
     2041 cggcgcaggc cggccaccag catgggctgc gccacggccg gaaagccggt gtgcacgcgg
     2101 tcgagcaggg cctgcacgcg cttgtccttg gccgatacgg gcacctcggg ttcggggcgg
     2161 ctcaggtcga tgggggcggg ctgggccggc gcgtgggcgg cgccgtcgaa gcccagcgcg
     2221 aaggcgcgca ggctggcctc gacgcccacg cccgcgcggc ggatggtggc ctcgaattct
     2281 tcgcggggga agggcagggc ctggctgccg gcgatggcgc cgaagaggct ggcgctgatc
     2341 acgctgccgg ccttttcggc ggcggcctgc aggtcgaagc agatgaagcg cctggcggcc
     2401 tcgcggccca cgtccagcac ctggttgggg tcggcgatgc cgttgccggg cgtggtcttc
     2461 tcgtcgatgg cgtagctgcg gtgcgacgag gtgatgagca cgctgcggtc gggcgtgacc
     2521 aggccgcgct gcatggcgcg gccggcttcc atgagctcgg cggcgacgat caggtcgacg
     2581 tcgccgggcg tgggcatgag cgccagggtg ggcgcgtggc cggcgcgctt gatgtcggcc
     2641 gcgggcacga tttccaggta atagatggtg gcgccggtgc gctgggcgac gccgggcacc
     2701 gaggtggtct gcgcccacca gccggcatgc tcggccatct cgacgatcca gtcggccagc
     2761 acgccgccgc cctggccgcc catggccagg atggcgatct tgatcgggtt ggacggaatc
     2821 aggtttgctg cggaatccat ggtgatcctt ctttacagcg cgtagcgggc caggcgggtt
     2881 tcgcggcggc gctgcagata gccgatgacc gcgccgcgca cgcggccggc gaagcgatcc
     2941 cagccggtgg ggttgtgcac cacgtcggcg cggtagaacg acgggcagag cacggcggcc
     3001 tcggcgactt cgccgcagtt gccgcagccc acgcagctgc tgtcgaccga ggcgaccggg
     3061 tcgaccttga gcggatcgcc gctgtccttg atggacagcg aggggcagcc cgacaggcgg
     3121 atgcaggcgt ggtcgcccga gcacacgtcg gcgtcgacgc cgaagcgttc cttgacgacg
     3181 cgcttgcctt ccttgaccgc cttgttgaag gcgggcttga cgcggcgctg cttgttcagc
     3241 atgcactcgg actgcgcgat gatgaccttg ggaccctcgg catcggtggt gagcgcctct
     3301 tcgaggaggt tgcgcgcctt ggtgacgtcg taggtgtggt cgatgacgcg cagccacttc
     3361 acgcccacgc cgcgcacggc ggcctcgatg gggttgttgg tggcgcgctc ggcgttctgg
     3421 gcgcgcgagg acaggatgtc ctggccgccg gtggccgacg aatagaagtt gtcgacgatg
     3481 acgatgacgc cgtcgaactt gttgaacacc gcgttgccga tgccggaggt caggccgttg
     3541 tgccagaagc ccccgtcgcc catgatggag atggaccgct tggcgccctt gatgttgaag
     3601 gccgagctgc tggccgcgcc caggccgtag cccatggtgg tggcgcccag gttgaacggc
     3661 ggcaggatgg agaacaggtg gcagccgatg tcgcagctga tgtggtgctc gccgagcttg
     3721 tcctgcgcga gggtgatggc cgagaagatc ggccgctcgg ggcagcccgt gcataggccg
     3781 gcggggcgct gcggaacgac cttggacagg ccggcgatgc gcttgtgcag ggtgatgggc
     3841 tgctcgaccg ggcgggggcg cgagacctcg gtgatctcca gctggttggc ggccttggcc
     3901 gcctgttcgt cggcggccac ggcgcgatgg gcgtgcaggg cctcgagctt ccacgccttg
     3961 aggaaggcct cgacgccgct gcgcaagacg gcggtggtgt attcgccggc caagggcagc
     4021 acgttcttgc cccacagcgg cgtggggatg tcggcgcggc gcaggatggt gtgcaggttc
     4081 tgctcgatgt agtcgggctg gccctcctcg agcagcagca ccgccttctt gtccttgcag
     4141 aatccgatga cctcgtcgtc gatgatgggg tagacgacgt tgagcacgta cagcgggatg
     4201 cggctgttgc cgaagtggtc ggccaggccc agcaactgga gggcgcggat ggcgttgttg
     4261 tagacgccgc cctgcacgat caggccgatg tcatcgatgt cgccctcgaa gaattcgttg
     4321 agcttgcgct ccttgatgaa cttgacggcg gcgggccagc gctgcttgat cttctcgtgc
     4381 tcgtgcacgt aggcggccgg cggcagcacg atgcggttga cgtcgcgcct gggcgattcc
     4441 atggcctcgg ccagggtgtg ggcggggcgc ttgttgtcct tggcgatgaa gcggccgtgc
     4501 acgtggcagg cgcgcacgcg cacctgcatc atcaccgggg tgttgctggc ttcggacagc
     4561 tcgaaggagt cttccaccga cttgaccatg gtttccaggt tggggcgcgg gtcgagcagc
     4621 cacatctgcg acttcatggc gaaggcgtgg gtgcgctcct gcatgatgga ggagccttcg
     4681 ccgtagtcct cgcccacgat caccacggcg ccgccggtga cgccgcccga ggccaggttg
     4741 gccagggcgt cggaggccac gttggtgccc acggtggact tccaggccac cgcgccgcgg
     4801 atggggtaca tgaccgaggc ggccagggtg gccgccgccg tggcctcgga ggcgctggtc
     4861 tcgaggtgca cgccgagctc ttcgaggatg tcgctggcat cggtgagcac gtcgatcagg
     4921 tgcgaaatgg gcgagccttg atagccgccg acatagccta cgcccgattg cagcagggct
     4981 ttggtaatgg ccaggatgcc ctcgccgcgg aactcttcgc ccgcgccgat gcgcagctgc
     5041 tggacttctt ttttaaacga tcgctcagcc atggaactcc cctaggttca aaatacttta
     5101 ggagtcaact atttaattgt caattgaaaa gatgaatttt catgcaaaca aaaaaatagc
     5161 tctaaaaaac aagggtgtat atactcgtta tccatcgacg gatgctgcgt cgcacaggct
     5221 tccaagtggt gctttttcgt ggattttttc tagggtaaac ccgaggattc tgcggccttt
     5281 gcagcgcaat ggtgcgcggt tggcgcggct cgtcagtctt gcacggattt ggtgcgctga
     5341 cgagccgccc ttcctgccca ttgtcgatgt tgatgccttg caacatgatg aaatccgaca
     5401 agcagcaaga caccggtccg gcgtcatcgt tcatcaacgg ctacctggcc tatctgttgg
     5461 cgcaggtgag ccacagcatc tcggccgagt tccatgccca ggcccatgag gccggcttgt
     5521 cggtgacgga atggcgggtg ctgagcagcc tgatgggcgg cagcggcttg accatcggcg
     5581 agctggcctt tctcgccatc accaagcagc ccaccttgag caaggtggtg cagcgcatgg
     5641 aggccaacgg cctggtggcg cgggccgagg tgcgcgccga ccggcgccag acccgcgtgt
     5701 cgctgaccgc ccgcggcacc aggctggccg agacgctgtg ccagcaggcc atgcggcatc
     5761 agatggccgt gctggagccc ttcggcgagg cgcgcagcgc ggaactgatg gagatgctgc
     5821 gcaccctcat cgtgctgcac gtgcccttgc cggcgagggg cgaggacgaa gctgcggatt
     5881 agctggactg cggctcgcgg gatgcgcggt gcaacggttc tgtcatccgc cgctcattgc
     5941 acgctgacgt gggtgaagtc ctgccgtccg aaagggctca cctggtagcc gctgacggtg
     6001 gcgcgcacga tgacggcggc caccggatag cctaggggga tccacagcgc ctggtcgtgg
     6061 atgatctgtt gcgcggcctc gtaggcttgc gtgcgtttcg cgggatcgga cgtggacttg
     6121 ccctgcgcga tcagcgcatc gagctgcgga tcgcaatagc gcgagaagtt ggtgccggac
     6181 tggaccgcat tgcagctgaa cagcgaggtc aagtaattgt ccgggtcgcc gttgtcgccg
     6241 gcccagccca tgaacagcgt gtcgtgctgg ccctgcttcg cttcccggat caactggccc
     6301 cattcgatgg tcttgatctc ggcccgcacg ccgatcttgg cgaagtcggc ctgcaggagc
     6361 tgcgcgccca cgcccgggtt gggattgagc acgctgcccg tggggcgaac ccagatcgtc
     6421 gtcgagaacc cgttcggata accggcctgc gccagcagct gcctggcgcg ggcgggatcg
     6481 taagggtagg gctggatcga gcggttatac ccccaggcgc tcgacggcca gggattgatc
     6541 gccgccgtgg ccgtatcgtc gaacacggcc ttcaggtagg aggcgcggtc gaacgcgaga
     6601 ttcagcgcct ggcgcacctt ggcgtcgtcc agcggctttt tctgggtatt gagcgcgacg
     6661 aaggccgtca tgaaggcagg cgtttcctcg accttcagcg ctttgtcgcc cttggccgcc
     6721 gcgatgtcct gtggcttggg cgacaaggcg atctggcatt cgccggcctt caccttctgg
     6781 gcgcgcacgt tcgcatccgg cgtgatcgcg tagatcagcg cgtcgatttt cggcttgccg
     6841 ccccagtagg acgggttgac cgcgtagcgg atcgccgcgt ccttggtata gctcctgagg
     6901 ctgaacggac cggtgcccac cggctgcgag ttcaggtccg ccggcttgcc ggcttgcagc
     6961 agctggttgg catactcggc tgaatagatc gaagcaaagc ccatcgtcag gatggacagg
     7021 aacgtggcgt tcggctcggt cagctcgaac ctgacggtat ggtcgtcgac cttcgagacg
     7081 gacttgatca gtttcgaaag tcccatcgac tgcgcatgcg ggaacccgcc cggcgtcgcc
     7141 ttgtaccagg ggtcgttcgg gtcgagcatg cgattgaagg tgaacatgac gtcgtccgcg
     7201 ccgaaggcgc gggtcggctt gaaccacgcc gtcgtctgga actgcacgtt gctgcgcaaa
     7261 tggaacgtgt acgccaggcc gtcgtcgctg atttcccatc gttcggcaag cgaggggctg
     7321 acctttttcg cggcctcgtc gtaggtcacg agctgatcga agaccacgtc ggccgatgcg
     7381 ttcgaggtca ccagcgaatt gaactggacg acatcgaagc cgtccggact ggactcggtg
     7441 cagacggtaa gcggttttgc gaaggccggc gcgatggcga acacaaacag accggcaagc
     7501 gcggcgcgca tgggaatgta caacttcgaa cgcgtgggca tggcctctcc gatataaatc
     7561 ctatgcaaca gccacagagt gtacagaatg ccatgccgcg tttttcgcag atgcttataa
     7621 gcctgttctt tgcgcgcagc ccgcgaggga gatcgaaacg cgcccgactc aatgcgaggg
     7681 gggtgcgccg tcatcgtcat cctcgtcctc gtcgtcatcc aggggcggca gttcctcctc
     7741 ctcgagcgag aagggcagca cctcggccag ttcgtccgac caggcgcatt cctcgccgtc
     7801 gccgtccagc ttgacgaagc gctcgccttc ggtggccgac agctggatgg ccagcaggta
     7861 gcggcagtcg taggtatcgg cgctttcggg caggccgccg ctctgcccca ggaggcgcgc
     7921 gtccttgccg cccagctgca cgtgcaggtg ttcctcgtcg ccggcggact tgtctttctc
     7981 gtcgagcgtg gagccgaagg tgacccactc cacgccgtct tcgtccacgc ccacttcggc
     8041 cagcaccgga cggcccaggt aggccgtcaa ggcatcggcg gtcttggcga gcatgtccag
     8101 ttcggcttgg gtaaagcgcg gtgcggcggc gggggatgag ggcggggtgg gcatgacgaa
     8161 agcaacctgt gggcgatcgc gcggtatgcg cggggggcgg ggcggcttat tgtagtgccg
     8221 ggcgccgctg ccggatcagg tagcgcagcc ccaccagggc gccgccgaac cccgagagcg
     8281 cggccatgcc gagcgcccag cgcggcccga agcggttggc gatccagccc accaggggcg
     8341 cgccgatcgg cgtgctgccc agcgccacgg ccaggaggat ggcgatcacg cggccccgca
     8401 tggcgggctc ggtggacaac tgcaccaggc tgttggccga ggtggtgacg gtctgggcgg
     8461 cgatgccgat aatgacgagc gccgcgccga agagccagta gttgggcatg agcgcggccg
     8521 tcgcgcagcc caggccgaaa acggcgacgc cgagataaag ccagtgcagc ccgggcttgt
     8581 ccctgccggc ggccagcagg gcgccgcaga ccgagccgac ggccatcatc gtggtgagca
     8641 ggccgtagcg gccggcatcg gcatgaaaga ccgagaccgc catcgtggcg atgaagatgg
     8701 ggaagttcag cccgaaggcg ccgatcagga agagcgtgat catgatggcg cgcaggtcct
     8761 cgcgctgcca gacgtagcgc aggccggcgg ccaggttgcc gcgctggcgc gcggcgcgct
     8821 ccttgctgtg cagttccgag gcgcgcagcc gcagcagggc gaagatgacc gcggcgaacg
     8881 agcacgcatt gagcaggaac acccagcccg tgcccatggc cgcgatgagc aggcccgcca
     8941 gggccggccc ggccatgcgg gccaggttga acgaggtgga gttcagggcc acggcgttgg
     9001 gcaggtcttc ctcgccgacg agctcggcca cgaaagtctg ccgcgcgggc atgtcgaaag
     9061 ccgcggcgca gcccagcgcc agggcaaagc cgtagacctg ccacagctgc gcggcgccgg
     9121 acagcgcgag caggccgagc cccagcgcca gggcgcccat ggcgccctgg gtgcagagca
     9181 gcagccggcg gcggtcgaag tggtcggcgg cgtagccggt caggccgatc agcagcacct
     9241 gcgggccgaa ttgcagggcc atggtgatgc cgaccgcgcg cgcattgtgc ggcgtgagat
     9301 cggtcaggac gagccagtcc tgggcggtgc gctgcatcca ggagccgatg ttggagatca
     9361 gcgcgcagcc ggcccacagg cggtagttgc ggatgcgcag cgaacggaac atcggcttca
     9421 cgcggtttac gcccgggtgc gctcgagcag ctccaggatg tcgcgcgttg cgccggtctc
     9481 gcccaggcgc gggaaaatgc gcgtgaggct gttgtggtgg gcgtcggcgt gcaggtcggt
     9541 catggcgtcg agggccagcg tcacgttgta gcccagctcg taggcctggc gcgcggtcta
     9601 ttcgacgccg atgctggtgg ccacgccggt cagcacgata tgcgtgacgc ccaggccctt
     9661 gaggtgggca tcgagatcgg tgtgcatgaa ggcgccccag gtgcgcttgg tgaccacgtg
     9721 gtcctgcggc tgctggttca gctcggggat caggtcggcc cagtcgggcg ccggagtgcc
     9781 ggcgcggcgc gcctgctcga tgcggcccgg cgcgccgccg gccacgttga cgagcacgac
     9841 gggcaggccg tgcttgcgga atgcctcggc gagcttggcc gagtgttcga tgaccgatgc
     9901 gatctgattc ggcccggtgg gcagggcgac gatgcccttc tgcaggtcga tgacgacgag
     9961 ggcgactttc ggatcgagtg tggtgacggg catggcgtgt ggctccagtg tgttgcggaa
    10021 aaggggttca ggcggcgttg cgcactttgc cgaagtgcac ggctgagaac acgcccagca
    10081 cggcgacgcc cgccagcatg gccagggtgt cggcattggc gtcgagcgcg gccgggtgcc
    10141 gggcgtacag cgccagcgcg acgagcagat tggcgaaccc ccagacgaag ttgaccagcg
    10201 gcgacgaaag gccgatgccg cgcggcttgg cgaaaggcgt gggaaagcgc tggccctgca
    10261 ggccgcagac caggtgcggc acgctattgc agaacagcat gccggtgatg aacagggaga
    10321 tcgggtgcat ggggaagggg gaggcgggac aagaggttgt cgaatagtag aggatatcct
    10381 ctactatttc aattcaagat ataggtgctg atttcagtgc ttttaaatta ccaaacgctt
    10441 acaggtgcta tgtatattga taatatgcga ggataagctc aatatttctc aagttttatc
    10501 gatccggggc tgcccatggc aactgcaacg tccgactccc gcgccgccgg ggtgcgcgcg
    10561 cccaagcgcg agcgtggccg caggcgcgtc gaggccatcc tggccgccgc catggaggtg
    10621 ttcgccgaaa agggctacga cgcggcgacg atgaccgaga tcgccgcgcg gtccgacacg
    10681 gccatcggct cgctgtatcg cttcttcgcc tccaaggagg tgctggccga cgccttgctg
    10741 ctgcgctatg cccagcacgt gggacagggc ctggaagaaa tcggccgcca ggttcccggc
    10801 ctgtcggccg agagcgtggc cggcctgctg gtggacttca tggtggcctt gcaggcgcag
    10861 cgccgcttcg cggtcgtgct ggtcgacgcg cgcggcggca gcggcaaccg gcgcgcgctg
    10921 ttccgccgca cgatacgggc gggcctggcc gagatcctgc tcaaggtgtc ggacaagctc
    10981 acgcccgcgc gcgcccagac catggcgccg gtgctgcagc atctgctcaa gggcgtggcc
    11041 ctggcggcgg acgaagagcc ggccgtccag gcggcgctga cgggcgaaat ccgcgagttg
    11101 gcgcggcttt atctgctgtc ggtgcggtaa ccgaccccgg ttggcgcgcg ttccggcggc
    11161 gtgctagggc cgcgcggccg agcgcggcgc ggatcggccg cgcagccagg cccacgcctg
    11221 ggccgcaagg taggcggcgc agctcagggc cgtgatccag aaactgcagg gccagtcggt
    11281 ccagtaggcc agggcgatgc cgagccaggc gcaggccagc gccagcgcgg ccgacagcgc
    11341 caggccgctg cccacgcggc gcgtcaggcg ctgcgccgtg gccggcgggc cgaccatcag
    11401 cgagaacacc agcaggatgc cgacgacctg cgtgctcagc acggtggcca gcgcggcgat
    11461 ggccatgaag agcgccgagt acgtgcgcag cgacaccccc cgggcctgcg ccagctcggg
    11521 ctgcaggctg gcaaagagca ggggccggga gatcgcggcc agtccggcca ggcaggccaa
    11581 gcccaggacc agcagcgcgc gcaccgtctc gccggagacg gccagcacat tgccgaacag
    11641 cagcgtgctg gcctgggtgg cataggcggt atagaagtgc aggaacagca agcccaggcc
    11701 cagcgagagc gacagcgcga tgccgatggc cacgtcgcgc tgcgccagcc gttcgcccag
    11761 caggccgatg cagacgccgc cggccagcgc ggcgatcagc atgccgttca gcggcgccag
    11821 gccgacgagc gcggcgccgg tggcgccgga aaaaccgatg tgcgagagcg catggcctgc
    11881 gaagctctgg ccgcgcagca ccaggaaata gccgaccatg gcggcgacca cggcgacgat
    11941 gccggccgcc gcgtaggcat tgcgcatgaa ttcgtagtcg agcaaggtgg tggtcctgca
    12001 aagggaaata gcggggaacg ctgcgtcagg ccgtcaggcg tgcgcctcgt gccggtgcgc
    12061 gtcggtctcg gcatcgacgc caccggcgat gacgaagagc cggcccgccg cgcgcacgac
    12121 gtcgatgcgc gtgccgtaca ggcgcgacag gacgggcccg gtgacgactt cctccaccgt
    12181 gcccagcgcc gcatggcgct ggcccaggta cagcacgcga tccagggccc ccagcagcgg
    12241 atggagctcg tgggcgctga gcagcacggt gatgcccaat tcggcctgca gcgccttgac
    12301 caggtcgacc atggcctgct ggcggcgcgg gtccaggctg acgaggggtt cgtcgagcag
    12361 cagcagctcg ggccgtccca gcagggcctg ggccagcagc aggcgttgtc gttcgccgcc
    12421 ggacaattcg gcaatggggc gctcggccag gtcgcgcgcg cccgtcaggt cgagcacgcg
    12481 gtcgacttcg cgccgcgcgg ccttgccctg ccaggccagg ccgaggcggt ggccgttcag
    12541 cgcgctggcc accacgtgcc ggccgctcag gcgcacggcg ggcgcgatgg cccgcgtctg
    12601 cggcaggtag ccgatggcgg ggttgccgcg ccgcgccgcg tcccccagca cgcgaatacg
    12661 gccggccgcg ggctcgacca ggcccagcac ggcgcgcatc agggtggtct tgccggcgcc
    12721 gttggcgccc agcacgccga tgaattcccg ctgctcgatg gccaggctca cgtcgtccag
    12781 caccgtgcgc ccgcccaggg ccaggcgcac gcgatcaagt tcgatggcag gcattacttc
    12841 gacttcgaca gggcttgctg cagccggtcc aggatgccca ggagccagtc ctggtaggcc
    12901 acgccttccg gttccgtctc ggtgacaggc agcacgggca cgccggcgcg ctcggctagc
    12961 cgctgcatgc gatcggccag cggatcggtg gcctggctgt tgtagaccag cagcttgacc
    13021 gcatgggtct gcaggctgcg ctcgaactcg gccacgtccg acgcgctggg ctcggtgtcg
    13081 ttcatcaccg ccagctggaa ggcggcgtgc cggttgagca ggcccgccgc gtcgatcagg
    13141 tagccgaaca ccggttcggt cgcggccacg ggggtgccgg catagcttgc gcgcagcgcg
    13201 tcgatcttgg ccagcacggg atgcatggat gcctggaagc gggccagccg ttcgcggaaa
    13261 ccggccgcgt gttcggggtc cgcgctcgcc agccgctcgg tgagcacggc ggcataggcc
    13321 agcatggtct gcgggtcgta ccagatgtgc gggttgtcgc cgtcctggcg gccggccagc
    13381 ggcgcgacgg tcagcacctg cgggccggcg tcgtgcccgg cctgcagcag tttttccatc
    13441 cagccgtcgt agccgatgcc gctttgcacg accaggcggg cggcggcgat ttcgcgcgcc
    13501 acggaaggcg aggcttcgaa caggtgcgga tcctggtcgg gcgattgcag gatgctgatg
    13561 accgacacgt catcgccgcc gatctgccgg gcgacgtcgc catagaaatt ctcggcggcg
    13621 acgatgggca cgggcgcggc ccaggcgccg gcgggcagca ggctgaggca ggcgaggaag
    13681 cggacgaagg ggaggatgcg gcgcatgggg gttccgtgag gaaaatcggg atgcccgatg
    13741 tcgatactgt aacgttatat tataacaaat aaagaagaag gagggcgtcc cgcaccgaaa
    13801 cggagccctt gccggccgga aagccagcgc gtccgcacgc ggcgacagac ccgcctaaaa
    13861 taggacttcg atgttctctt ttcttaccta tccccgatgg cccaatacgt ctacaccatg
    13921 aatcgcgtcg gcaagatcgt gccgcccaag cggcagatcc tgcgcgacat ctcgctttcc
    13981 ttttttcccg gcgccaagat cggcgtgctg ggcctgaacg gctcgggcaa gtccaccttg
    14041 ctcaagatca tggccggcgt ggacaaggag atcgagggcg aagccattcc catgccgggc
    14101 ctgaacatcg gctacctgcc gcaggaaccg caactcgacc ccgggcacac ggtgcgccag
    14161 gcggtggaag aaggcctggg cgcggcgatg gccgccaaga agcgcctgga cgaggtgtac
    14221 gccgcctatg ccgaacccga cgcggacttc gacgcgctgg ccgccgagca ggccgacctg
    14281 gaagccatca tcgcggccgc cgcctccagc ggcgccgacg acatcgagca ccagatggaa
    14341 atcgcggccg acgcgctgcg cctgccgccc tgggacgcca tcgtgggcaa tctgtccggc
    14401 ggcgagaagc gccgcgtggc gctgtgccgc ctgctgctgt ccaagcccga catgctgctg
    14461 ctcgacgagc ccaccaacca cctggatgcg gaaagcgtgg agtggctgga gcagttcctg
    14521 cacaaattcc ccggcaccgt ggtggccgtg acccacgacc gctacttcct cgacaacgcc
    14581 gccgaatgga tcctcgaact ggaccgcggc cacggcatcc cctggaaggg caactacagc
    14641 tcctggctgg agcagaagga cgcccgcctc aagcaggaag agtcctccga gtcggcccgc
    14701 cagaagacca tcaagaaaga actggagtgg gtgcgccaga accccaaggg ccgccaggcc
    14761 aaggccaagg cgcgcatcgc ccgcttcgag gaactgtcgt cctacgaata ccagaagcgc
    14821 aacgagaccc aggaaatctt catccccgtg ggcgagcgcc tgggcaatga agccatcgag
    14881 ttcaacggcg tgagcaaggc ctacggcgat cgcctgctga tcgacaatct cagcttcaag
    14941 gtgcctcctg gcgccatcat cggcatcatc ggtcccaacg gcgcgggcaa gtcgacgctg
    15001 ttccgcatga tcgccggcaa agagcagccc gactcgggcg aggtcgcgat cggccagacg
    15061 gtcaagctgg cctatgtcga ccagtcgcgc gacgcgctgg aggacaagaa gacggtgttc
    15121 gacgccattt ccgacggcgc cgacctgctc accgtaggca agttcgagat gtcctcgcgc
    15181 gcctacctgg gccgcttcaa cttcaagggc agcgaccaga gcaagatcgt gggccagctg
    15241 tcgggcggcg aacgcggccg cctgcacatg gccaagacgc tgatcgccgg cggcaacgtg
    15301 ctgctgctgg acgaaccgtc caacgacctc gacgtggaaa cgctgcgcgc gctggaagac
    15361 gcgttgctcg agttccccgg cagcgtgatg gtcatcagcc atgatcgctg gttcctggac
    15421 cgcatcgcca cgcacatcct ggccttcgaa ggcgattcgc aggtggtgtt cttcgacggc
    15481 aactaccagg aatacgaagc cgacaaggtc aggcgcctgg gcgccgaggg cgcgaaaccg
    15541 aagcgtttgc gctacaaggc tttgaaataa ttgtgttgag ttcttaagac gcatcgccgc
    15601 cgcggccagc ctgccgggca gcgcggcggc ttcgctgcgt atagacacaa cttattggcg
    15661 atgctcacac cattgggggc gtcttctgga atcctgcgga taagaacaag ggcgcgcgag
    15721 cgccatcgtc gtattttcgg ctgccgtgct cccttgactg catcaaaaac aggcagggag
    15781 tttttcatga acattcgcac gctttcccag gtcagcctcg ccgcgctgct ggccatttcc
    15841 atggccggct gttcgtcgtg ggatcacatg agccgccgtt ccaagaccat ggtgggcggc
    15901 gcggcgctgg gcggtttggc gggcgcggtc atcaccggcg gcggcgtgct gggcaccgtg
    15961 ggcggcgcgg ccatcggcgg cgtcgttggc gaccaggtcg gcaagcgctg agcgccgctt
    16021 cgcctttctt gttcctttcg tttctccatg catccaggat tttctcctca cgggagtaac
    16081 tgaattcaat gggcgccgga ttgtgcccgg cccgggaagc tcctagcatg tctgtgccgc
    16141 ccgaccaggc ccaggccatg ccgtggccgg ccgcgcgggc ggcggcacca gggagaatgg
    16201 gatgagactg acgggaagaa tgctgttatt gacggccggc tgcgtcctgg ccaccggcca
    16261 ggcctttgcg gccgccaagc cggtgcggat cggcgtcacc acgatcctga gcggccctta
    16321 tgcggaccgc gggcaaagcg agcagtacgg cgtcgagctt gccttgcagc agatcaacca
    16381 ggccgggggc gtcctcggtc ggcccgtgga ggccttttac gccgacaatg ccgacgacac
    16441 cgcgaagggc ctcgcggccg ccaagcggct catcgagcag cagcgcgtcc ccgtgctgat
    16501 cggcgcgctg gccacgccgg tgacgcgcgc catcatgccg gtggccaacg cggcgaaggt
    16561 cccgctcgtg atcgacatct cggcgggcca cgagttcgtc gacgcggcgg gttcgggcgg
    16621 ctatagctac gtgttcaaga ccagcccttc cgacgtcgac gtggccgccg ggatgatgca
    16681 gtggctcaag ggcaagcgcg tgggcagcgt cgccatcgtc gccgacgaca atgccttcaa
    16741 tctcgccaat gcggcgtcca tggcgcaggc cgcggccgcc gcgggcatca aggtcctgtc
    16801 gtccgacgtc gtcgccaagg ggacgaccga tctctccggc ctgctgggca agatcggcgc
    16861 cgcgcatccg gatcgcatcg tcaccgtcct cggtccgtcc agcggcctgt tcttcaaggc
    16921 ctacgagcag gacgcggcgc atatccccct ggccggccgc atcgatttct cggcggtcaa
    16981 ggcgagcctg tcgccgcagt tcctggcggc cggcgggctc gaccaggcgg cgggcgtctc
    17041 ggtgtacacg cccgcgctcg acacgcccgc cgtgcgcgcc ttcgccatgg cctacgaaga
    17101 caaatacggt ctcgccccga cccagcgctc cctcctgggg ttcgaggcgg cgcgcctggt
    17161 cgtcgatgcg atccggcgcg cgaagtcgga cgaccccgcc gcgatcgagc gcgcgctcaa
    17221 gtcgacgaaa atgccgtccc tgctgggcgg cacgttcgcg atggacgcgc acaaccatgc
    17281 ccacaccggg atgcagatcc tgggctggcg cggcaacggg gtcgccgtgc tcgagcgcat
    17341 cggcgcgtag gccggccgcc tgcgtcagta gtggatgccg cccgacacct cgatgcgctg
    17401 ggcgttgatc cagcggtttt catcggccag cagcatggcg atggcgccgc cgatgtcgtc
    17461 gggcaagccg acgcggccca ggggggtctg cttggcgatc gcggcgttca tctgcgcgtt
    17521 gtcgcgcacc acgccgccgc cgaagtcggt cgcgatggcg cccggggcca ccgtgttcac
    17581 ggcgatgccg cgcgggccca gttccagggc gagatagcgc gtgagcacct ccacgccgcc
    17641 cttcatggac gcgtaggcgg cgaagcccgg cagcgagaag cgggccaggc cgctggacag
    17701 gttgacgatg cggccgccgt cggcgatgag cggcagcagg gcctggatga ggaagaaggg
    17761 ggccttgaga tgctggcgca tcatggtgtc gaactgggcc tcggtggtgt cggccacgct
    17821 ggcgtggatg ccggtgccgg cattgttgac caggtagtcg aactgttcgc gctgccagtg
    17881 ccgggccagc gcggccttga cctgttcggc gaaggcggca aaggccgcga cgtcttccac
    17941 gttgagctgc agggccacgg cgcgggcgcc cagggcggcg gcctcggcca cgaccttgtc
    18001 ggcctcggcc ttgttgctgt tgtaggtgaa gacggcgtcg atgccgtggc gggcggcgtt
    18061 gagcagggtg cttttgccca ggccgcggct ggcgccggtg atgagcagaa tcttgcgggt
    18121 catggatggc tcctggtgag gtgtgcccgt tgcgccggac acgatggaag acatcataat
    18181 tgggtgattt aattggataa atcaggtaaa aatgattttt ctgttcaaga aaatataaca
    18241 atacctgagt gcaagtccga atcatgagtc actacgcatg agtccccgcg catgaatcgc
    18301 tacgacgcca tgcggctttt cgtccgcgcc gcggaaacgt ccaatttcac gcaggcggcc
    18361 gacagcctgg gcgtgcccaa ggcgtcgatg tcgaatgccg tgcggcagat ggaaacccag
    18421 ctgggcgtgc gcctgttcac gcgcaccacg cgccgtgtcg cgctcacgca ggaaggccgc
    18481 atgtgcctgg accgctgcaa ggacatcctg gccgacatcg acgacctgga gtcgctgttc
    18541 tccaccgaca agaccgtcag cgggctgctg cgcgtggaca tgccgcatgc cctggcctgc
    18601 gcgctggtga ttcccaggct gccggatttt ttcgatcagc atccgggcct ggaactggag
    18661 ctgtcgacca ccgatcgccg cgtggacctg ctgcgcgagg gcttcgactg cgtgctgcgc
    18721 gtcggggcgg tgcaggagtc ttcgctgatc gcccggccgc tgggcgagct gccgcagatc
    18781 aactgcgcta gtccgcggta cctggcgcgc ttcggcgagc cgcgcacgct ggaagacctg
    18841 cgcgggcacc ggctggtgat gtacgtcccc gagcccggca tgcgcgaccc gggctggcag
    18901 tatcacgatg gcgagcgcta cgcgcatgtg cccatgggcg gcgtgctggc ggtgaacggc
    18961 gtggatgcct accaggaggc ctgcctggcc ggattgggca tgatccaggt gccggcctac
    19021 ggtgtgcagg cctacctgga ctcgggacga ctcaaggagg tgatgccggc ccaccaggcg
    19081 gcaccgctgc cggtgacgct gctttacgcg gaccggcggc acgcgccctt gcgggtgcgc
    19141 gtgttcatgc agtggctggc ggagctggtg gccggggcgt atccaggcgt cccgcatgcg
    19201 ggacgcccgt agcgcggcag gctcaggcct cgggctgcgg catctcgatc ttgacctcga
    19261 gcacttcgag ggtgtcctgc cgttcgagat ggaccttgat gtcctcggga ttgatcttga
    19321 cgtacttgga gatcacggcc acgagttctt tctgcagctg gggcagatag tcgggcgagg
    19381 cattgcggcc cgagcgttcg tgcgccagga tgatttgcag gcgttccttg gcgacggtgg
    19441 ccgtcttttt cttctcgcct atcaggaaag acaggaagga catggattat ttccctccga
    19501 agatacgctt gagcagcccc ggcttgctgt agtcggtgaa acgcagcgcg cgttcctcgc
    19561 ccagatagcg ggccaccacg tcctggtagg cctcggatac gtcggtgcct tggagatgga
    19621 tggcgggcag gccctggttg gacgcggcca gcaccgattc ggactccggg atcacgccga
    19681 tgagcttgat gcgcaggatg tcttcgatgt cggtgagcga gagcatttcg ccgtcgctga
    19741 cgcgcttggg gttgtagcgg gtgagcagca ggtattcctt gaccggctcg tcgctttcga
    19801 tggcgcggcg cgacttggcg gccaggatgc ccaggatgcg gtcggagtcg cgcaccgagg
    19861 agacctcggg gttggtgacc accagcgcgt cgtcggcgaa ataggcggcc atcagcgcgc
    19921 ccgtctcgat gccggcgggc gagtcgcaga cgatgtagtc aaagcccatt tccttgagat
    19981 cgtccatcac cttgcccacg ccttcctggg tgagggcgtc cttgtcgcgg gtttgcgagg
    20041 cgggcagcac gaagagattt tcgagctgct tgtccttgat gagagcctgc ttgagcgagg
    20101 cctcgccctg gatgacgttg acgaagtcgt acaccacgcg gcgttcgcag cccatgatga
    20161 ggtccaggtt gcgcagaccg acgtcgaaat cgatcacggc ggtcttgtgg ccacgcatgg
    20221 ccaggccgga agagaagctt gcgctggtcg tggttttacc cacgccaccc ttgccggaag
    20281 tcaccaccac gatacgtgcc atggtcgttt gatcctttta tttaaagtcg cgttatcgta
    20341 aagcaaaaag ccccacgggc ctttttccaa atgtgtgccg ggcgcgcctg ccgcgcgggg
    20401 gggggcgccc atcggcgcct cactcgccga tggcctcgat cttcagcgtc tcgccgtcca
    20461 gcgagaccag ggccggcttg ccgtgcaccc gctggtcgag cttgtcctcg accacgcggt
    20521 agacgccggc caccgccagc agctcggggt cgagctgcgt ggtgaagatg cgggccgagg
    20581 tgtcgccgcg cgcgccggcc atggccttgc cgcgcagcgg cccgtacacg tgcacgttgc
    20641 cgtcggcgat gacctcggct ccctggctga ccatgccgat caccaccagg tcggtgttgc
    20701 gcgcatagac gcgctggccc gagcgcaggg ggcggttgac gatcatggcc gcgctggagg
    20761 gcgcgggcgg cgtgtcggcg gcgacgggga ttcccggaac ggccggcgtc ggcgcggcca
    20821 cgggcggttc ggcgggatcg gccggttcgg ccgcttgcgc ggccatcggt gccgccgccg
    20881 gcatggcggc tgccgcgcgc ggcgtggccg tggacaggtc caccggcacc aggcctgcct
    20941 ggcgggcggc ctcaagattg ccgccttcgg ccaccacgcc gatggccggc aggttatagc
    21001 gccgcagggc ggccagcagg tgtgtccagt cgatggcgtc ctcgacgcgg ctggcgtcga
    21061 tcacgacg
//
LOCUS       DASWPO010000348        34326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_170989, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000348 DASWPO010000000
VERSION     DASWPO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 34326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34326
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170989"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VGC69_18505"
     CDS             <1..762
                     /locus_tag="VGC69_18505"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY0297325.1"
                     /translation="PGAGGAVGTQTLVSSAPDGYTLELAAPSPLIVAPYLIKNLSYDP
                     QKDLAPISLIAVVPAVLLVKEDSPIHTVQDLLDMARKEPGKLTFSSSGLGGTAHLSAQ
                     MLKVLANVDMLHVPYKGTAPAIEAVMSGQVTLTFADMTAGLAYAKNHQLRAVAVTTPT
                     RTPALPDVPTVAETVPGYEATVWYGVFAPKGTPQPIIDKLNSEIVKFVHSPDVDKILL
                     NEGAEPKGSTPEELRAFMRDEAARWGKVLQASGAQ"
     gene            complement(794..1894)
                     /locus_tag="VGC69_18510"
     CDS             complement(794..1894)
                     /locus_tag="VGC69_18510"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY0297326.1"
                     /translation="MLLINNEVVEHVLTMPECIEAQEQAFAGLLTGEAVGRPRLDMYV
                     PCERDDGYYRWGSVEGASGGVLAVRLKSDILVWPRDHAAGGSEQKYCMQPGTYCGLVF
                     LFSTQNGEPLALLNDGHLQHMRVGGAAGIGTRLLSRADSRELGIIGSGGMARTFLEAI
                     LAVRGIQRVRVYSRSKDKREAFAAETSRRYGIEVVPVDAPRDAVRGADIVATCTDSMT
                     PVLEPDWLEPGMHVVVLTPREISPDIVQRFDVAVQQGREDLPMPETERFRKGISGSPG
                     AFVAGTAEEQQRLPQPKKSRLPTEDWPVYTDVISGKAAGRTRDEQISFYYTVGNWGVQ
                     FSSVGGLVYRKAKAQGLGHDLPLEWFMQTIRN"
     gene            2174..2890
                     /locus_tag="VGC69_18515"
     CDS             2174..2890
                     /locus_tag="VGC69_18515"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0297327.1"
                     /translation="MIKKVQDSNLLLGEAAYESVREAILANVLKPGERVSEYKVAEWL
                     GISRTPAREGLRRLENEGLLAQHPRRGLVVASIDDAAVQELYAVRELLESEAAEQAAR
                     HASAAEIATLKHLVEIEASLSEKPEQMYEHNRTFHEQVYRAAHNRFLLKFLLLTADTL
                     STYRNVSTLVFKERRDEVLAEHRELCDAIARRDEAAARAVATRHVQNALRARTRVQHS
                     KLTSQAQQQRGPADLAKLEP"
     gene            complement(2908..4251)
                     /locus_tag="VGC69_18520"
     CDS             complement(2908..4251)
                     /locus_tag="VGC69_18520"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0297328.1"
                     /translation="MPTASQELAAYAAGLRGSDVPDDVRERARACIADTLACSVYGAR
                     FPWSAATAAYARRYGGLGPCTVFGVAGHGVAAPAAALANGAAAHAFEQDSLRFPGAGV
                     HPGATLVPAIAAACQETGADGETALRAFVAACEVLFRIGAATHHSSEKLGFHAPGLTG
                     PYGATVAAGVVYGLDAERIAHALGIAGSMSAGLLAFTKAGQGAMVKRLHMGRACEAGI
                     VAARLASEGYTGPETILEGKFGFLQTYCSEGEAALLTAGLGREWESRKICMKRYPCHV
                     TAQPAVQALRDMMREHGFAAGDVAGLSLRMSEKVVSHHDIRAPGDVMTAQYSVPFCVA
                     LALHRDPEDPRSFDESALADADIRRMCALIDLGAAQDLPTAWSARIDVALHDGRRLQA
                     LADSFTGMPQDPPDQASLRRRFVLLTEDTLGSQASMAWYEALAGLENQAVFPAPA"
     gene            complement(4265..5665)
                     /locus_tag="VGC69_18525"
     CDS             complement(4265..5665)
                     /locus_tag="VGC69_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0297329.1"
                     /translation="MSQSTLSDNRYTQGIAQFVSGLRYEAIPPEVVTRIKLLMLDSLG
                     CALYGAQLEWSRILQKTLGGLDTTQGCAVWGTGQKLSAPHAALVNGTQVQGFELDDVH
                     RQGVLHVGAVVLPALVSIAEMRPGMSGREFLTSAVAGYEIGPRVGICMGPEHIAQGWH
                     SGATLGVFSAAAGAARGLGLDVDRTVHALGIAGTQAAGLMAAQYGAMVKRMHAGRSSQ
                     SGLYGALFAEQGFTGIVNVLESEYGGYCTTFSRSTDRFKLEELTAGLGEVWQTMGIAL
                     KFYSCVGSNHSTLDAIRALQAEHPFGEADVEKIVVHGSQVTMDHVGWKYEPQGLTSAQ
                     LNLPYCVATWLLDGDCFVDQFTEDKVADPERMRVAGKVQVAHDAEITAKGSKFRHMVR
                     VEVFLKNGTRLARTVEAGRGNERDFASEADIVEKFDKLALHALQRPQAERIRDWVLNM
                     DQRADAAELPRLLAKA"
     gene            5782..6810
                     /locus_tag="VGC69_18530"
     CDS             5782..6810
                     /locus_tag="VGC69_18530"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297330.1"
                     /translation="MLRPLPPGGETVEIRRRQFIARGLALAAAPLAPRAALSATDADG
                     YPSHPMRLVIPFAPGGGTDIIGREIAHQLSLAWKQPVIVENRAGGNGTIGLANVANSP
                     ADGYSLSMVTASASVNVTLQGRTLPYSLIDGFAPITQVTSQPYLLVVSPALGVKSVQE
                     LLDLGRKRATPLTFGSSGIGGLSHLSGALFSSLSKVPMTHVPYKGGAPAMADVASRQI
                     DMLFSTQIEANGLIQGGMLQALAVTTARRAAASPNLPTMQEAGVAGYEVAGWYGLLAP
                     AHTPPEIVAKLNTEVNRVLTLGDVRQKIHADGSEPAGSTSAQFAAHIRSEVGRWRALI
                     AQLNIPTQ"
     gene            6807..8237
                     /locus_tag="VGC69_18535"
     CDS             6807..8237
                     /locus_tag="VGC69_18535"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY0297331.1"
                     /translation="MTTATEDETLAPPVDAAPGLARELGARVAGLSFERLSADALRWA
                     RMGILDTIGVTLAGAREDAPRLAARALDLGEGPSLVLGARRRVGALDAALINGTASHA
                     LDFDDCNNTLGGHPSAPVLSALIPLAEQLGSSGRDFVTAYVAGFEAETKIGLAVNFHH
                     YTKGWHPTATLGTFGAAAACARLMGLDAAGTATALALAASFASGIKANFGTMTKPLHV
                     GHCARNGLYAARLAREGFTANAARVFEHKQGFLDVFNGPGTYDTQRALDAWAKPLDIV
                     EPGIAVKQYPCCGSTHPALDAMLELVRRHGPRLEQVERVDAWIHARRLAHTNRPRPNS
                     PLDAKFSLQYVLARALLDGHVGVNDFIESAYRQPEVLALLPRIHVAAYNETGVGGFPE
                     ANHFGGLVRITLRDGSVLEAQVDQPLGRTSANPLPPALLRDKFSLCASAVLAPPAIPA
                     IAETIERIETLNSVGELTAQIHRATT"
     gene            8269..9765
                     /gene="tcuA"
                     /locus_tag="VGC69_18540"
     CDS             8269..9765
                     /gene="tcuA"
                     /locus_tag="VGC69_18540"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEY0297332.1"
                     /translation="MEQPTHHYDVIVVGKGNAALCAALSAQENGARVAVLEAASQDES
                     GGNSRFAGGVMRFAYASVDDLKTVTELTEEEIATSDYFTNTTDEFFDDLFRLTSFRTD
                     PQLSEILVTRSLETMQWLRGKGVKFVPNYGRQSSLVNGRRKFFGRLPIEVSGGGAGLV
                     AFLDRAAQKAGIDVHYNTRVQQLVLDGERVAGVLANQEGEQVEFLGKAVVLACGGFEA
                     NPEMRARYLGPGWELAKVRGTRFNQGDGLRMALGSGAASHGNWSGCHATGWDLNAPEF
                     GDVDVGDQFQKHSYIFGLLINAHGKRFVDEGLDFHSFTYAKYGGEVLKQPGQFAWQVF
                     DDKVTKLLRSEYRIKMITKATADTLEDLAQKLEGVDPEAFLQTVRAYNAAVKADVPFD
                     HTIKDGRGTVGIEPPKSNWAQALDTPPYHAYATTCGITFTFGGLRIDPDNGQVLDVHL
                     TPMPGLYCAGEMVGGLFYFNYPSGTGLVSGAVFGRIAGAGAAKEAAAG"
     gene            9963..11339
                     /locus_tag="VGC69_18545"
     CDS             9963..11339
                     /locus_tag="VGC69_18545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297333.1"
                     /translation="MTETLAIQPEAMPASRRRWAMFCVLLALALSSLDSAIANIALPT
                     IARTLATSNSNSIWIVNAYQLSVMVCLLPVAALGESLGLRRIYAAGLTIFTLASLCCA
                     LSPTITVLVCSRLAQGVGGACMSVSSVALVRGIYPRETVRNGYALVALAVAASGALGP
                     TIAALILSVASWPWLFLVNLPFGIIAVPLLLAVVPPDVRHPRPFDWIGAGLNALAFGL
                     VISGVHSLGDESIGVAVAMIVAGIACLGLLARQQMRQPQPLLPLDLLRIPQFALSVAA
                     SVCTYAAQILAYVSIPFLFEVNLHLTPVETGLLVTPWPLLVAFTAPLAGRLMSRHPAD
                     LIASVGLLVLACGLVLMAALPAEPAYGNIVWRLGVCGIGFGLFQTPNNTLMMTTGPAK
                     RSSAASGLNAVARFMGMALGSAVAAVILGVGGQHATIACLEVGAAFALVGAAASGARR
                     KWQGQGLS"
     gene            complement(11350..11523)
                     /locus_tag="VGC69_18550"
     CDS             complement(11350..11523)
                     /locus_tag="VGC69_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297334.1"
                     /translation="MSLFDLDLIPVIGGIGIVLLLVGFNLHDYKAGPVLMPLGMAMVL
                     GTIVLKIYIAAHR"
     gene            complement(11641..12624)
                     /locus_tag="VGC69_18555"
     CDS             complement(11641..12624)
                     /locus_tag="VGC69_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY0297335.1"
                     /translation="MSRRIAVIGAGGTIAMEGAHSFDWVDYGDSGIIHAVDEVVAGMS
                     FGLPFVDVVSHPFRALPGTGISPKDWLDLAREIQAVAASGEVDGIVVTHGTATLEETA
                     FFLHCTVRCGMPVVVCGSQRPPNTVSSDAVSSVRNALAAAAQAPVGVYVAMNGYLYSP
                     ADVSKTANHALDAFDAPEFGPLGRIEASGMLSMRRGALPPARVFDGSAWSAQDFARVD
                     VAYSYAGADGVAIDAYAAAGCAGLISVGFPPGRCTPAERAALLRAVRQGVTVVQASRA
                     LRGAVPLQAYNTADGILSGGGLSPGKLRILLMLALAGKLPRQEIQALLLSA"
     gene            complement(12621..12944)
                     /locus_tag="VGC69_18560"
     CDS             complement(12621..12944)
                     /locus_tag="VGC69_18560"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY0297336.1"
                     /translation="MSDTQQWSDVCQAGEVDEGDAKAFHVNGKRLCVVNDGKGYYALD
                     DVCTHGMAFLSDGFCDPEEGVIECPLHGGLFSYCDGSVKGAPAERPTRVYPILVENDV
                     VKVRL"
     gene            complement(13009..13548)
                     /locus_tag="VGC69_18565"
     CDS             complement(13009..13548)
                     /locus_tag="VGC69_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0297337.1"
                     /translation="MDPSADLARRLARLEAREQIDELMQRYAAAADRKYTALRQKQSA
                     DIVRAAAREQAECFTADARWAGGRFGGDLVGRQAIAAFFETSPWLFTAHHYGHPIMRI
                     DASGRSAAVRWRLLELGIREQDGKVALLTGVVDQDCAADDEGWRIARMQFASLHALEL
                     AGRPEQLRCLIPEGEPFDL"
     gene            complement(13582..13863)
                     /locus_tag="VGC69_18570"
     CDS             complement(13582..13863)
                     /locus_tag="VGC69_18570"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0297338.1"
                     /translation="MSVLCRVEQSGMGKAPFGIEGERLAARMERIRGTFDEAPGAEEM
                     IAVLDGEARLVCDDEDYALRAGLGLLVPAGMPLHWEVPGEAVVYRVKVK"
     gene            complement(13860..14285)
                     /locus_tag="VGC69_18575"
     CDS             complement(13860..14285)
                     /locus_tag="VGC69_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0297339.1"
                     /translation="MSKCQLFSFSEDLREVVPGGLYMQSVVGETMSVGVINFRLPAGP
                     GIEPKSHAHGEECTLQIRGGCTVNLGHSVTEPEGSVLLEEGSVMVMPAEQAHSGINHF
                     DAQGWSLRLNIVTPPRAEYGSKGRSKVYYPSADEKGASK"
     gene            complement(14297..14647)
                     /locus_tag="VGC69_18580"
     CDS             complement(14297..14647)
                     /locus_tag="VGC69_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297340.1"
                     /translation="MSNKLPESFGYLEPWNDWCLATETERNHRRVSLKFPEIKAFADA
                     MLPAVDAACRWIDAHQVDGKYDEQTLNLFHMLLSLAEVAPAIESYDPSVVVTDGYDSR
                     LFAADERHTLRPAI"
     gene            complement(14664..15956)
                     /locus_tag="VGC69_18585"
     CDS             complement(14664..15956)
                     /locus_tag="VGC69_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0297341.1"
                     /translation="MQAQPARLHDRYPELGRGPLPVAPYTDPAYFELEKKYLFKKTWL
                     HVGRVEEIPKVGDYIVREIPALDSSIVIARDRSKGIHAFHNVCSHRLNRIAYEPAGNT
                     RKFFCKFHGWAYDLDGSLSGVPDEKSFIDGLDKSRYGLTQVSVDTWNGFLFINLDPNP
                     SVSLKNFMEPMYAGFESYPFERFTACYAWNAVVDCNWKVALDAFQETYHVGYVHGRSI
                     AYALDQDEEGMLHPIDGVCGDLHRRLSIAGNPNTVYGNPQAATQGAAATDAAQASQGS
                     GRAIAAKALAVAANGTRLDFSDVKLPELLNWTRHPYWAFDINVVFPDFYLSCRPNYFQ
                     AYNFRPLTHNKFLFEARVYYPEMKTAGGRFYQEYMKVALRDVLLEDLSTLEMTQKAAE
                     TGAKSEMIIQDYELMVRHQAVVIDKIIQEGLRRDGQAA"
     gene            16068..16742
                     /locus_tag="VGC69_18590"
     CDS             16068..16742
                     /locus_tag="VGC69_18590"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0297342.1"
                     /translation="MSQTIVRVADLRQQVYDALKARITTGKFPSDAHLFEISLAKELN
                     VSRTPVREALAMLVRDGLLVQLARGFRFPRFSEQEIIEIIEIRLLLEPYAIERMILDN
                     TRAAMAALGAGMRRDLLAAGGGDARAYAEVHRKVRDDIYAHVGNRQLADAIAKYEDSI
                     HYIRVSTLNEEESRQVSYDGMLRLADAIARGEAGTARKLMEQQLINARLAFLDHVTAA
                     AELEAQ"
     gene            16739..17452
                     /locus_tag="VGC69_18595"
     CDS             16739..17452
                     /locus_tag="VGC69_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylmalonate decarboxylase"
                     /protein_id="HEY0297343.1"
                     /translation="MSLADKAYGYRARVGQLYPSGGLCDFEMQRMAPEGVQFVTTRMP
                     FRDTSKESDLALVRDLENNARLLADAQVGLIAFNCTAASLLCGPAVIRERIRAATGID
                     SITTIEAVEDALRRFSARRIGLFTPYRREVIESEKEYLVQQGYEICAEAHIECLDPVQ
                     QGSIPPATWLDLARRTDVSGCDTLLFSCAGIYLSPVLAGIEQATGKPVVSSNAALLWR
                     ILRELGIAGDPAHGRLFAA"
     gene            complement(17493..17954)
                     /locus_tag="VGC69_18600"
     CDS             complement(17493..17954)
                     /locus_tag="VGC69_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0297344.1"
                     /translation="MKPDAAADAYLVSEQVGHLLRRAYQRHVAIFQQRIPDTQLTSAQ
                     FVVLCAVHEQDACSLAEIVKHTAIDQATVRGVIDRLKVKKLISVAHDPQDKRKVLVTL
                     TAAGTALVRKTVPFAQEITESTFGALNPAERVAIVYLLRKMCDAAAPDAAA"
     gene            complement(17954..19087)
                     /locus_tag="VGC69_18605"
     CDS             complement(17954..19087)
                     /locus_tag="VGC69_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0297345.1"
                     /translation="MQGKPRIAIIGAGLGGAACAGLMARAGFNVRVYEQAPAFARLGA
                     GIHVGPNVMKVLRRIGIEDALSEQGSHPEAWYSRDGKTGEVMARIPLGDYAVERYGAA
                     YLTVHRGDFHDLLVKAIPSDAIAFGKQLTHVEDTGSVVRLGFADGSWEEADLAIGADG
                     VYSKLREYLLGPEQPKYAGYVGHRAVFPTPVDSGALPFDKCCKWWADDRHMMVYYLTG
                     KRDEIYYVTGVPEEGWDAGDRFVPSSKEEMREVFAGWHSTVQALIENTVEISKWSFLE
                     RDPLPLWSRGRIVLLGDACHPMKPHMAQGAAMAIEDAAMLTRVFEQVGFTDYEAAFKL
                     YADNRIERASKVQFVSHNNTWLRTNEDPAWCFGYDVFSEPLKG"
     gene            19289..21586
                     /locus_tag="VGC69_18610"
     CDS             19289..21586
                     /locus_tag="VGC69_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0297346.1"
                     /translation="MNLPAPRALHQDLLSAPNTLLVVRDPMPAAKPAPGQPGFASDYV
                     PDTPDIFIAVLPDGRVQAFNGHVDLGTGIRTALAQLAAEELDVAFGRVEMVLGHTEAA
                     PNQGPTIASASIQISAVPLQRAAAQARAWLLARAAALLDADPGALTVDDARISAPNGR
                     STDYWQVLAQGGGRIMLAAPADPVALKPASQHKLIGRGVARVDIPAKATGGLTFVHDV
                     RLPGMLHGRVVRPPYTGRDAGDFVGRSLRRVDEASIAHLPGVVKVVVRGDFVGVVAER
                     EEQAIRAARELRVEWTTPPAAPALGNLDEALLAIPAVRRQLKNEGDIEATLADPAMKA
                     LSATYVWPYQMHASIGPSCSVADWRDERLTVWSGTQNPHMLRIDLHRLLDLGEDRIEI
                     VRMEVAGCYGRNCADDVCADAALLARAVGRPVRVQLSRAQEHTWEPKGTAQRMQVSGA
                     VDAQGNLQAYDFVNRYPSNDAPTLALLLTGVVDPAPRRMEMGDRTSMPPYRYPNLRVA
                     CDDVPAIVRASWLRGVSALPNSFAHDCHIDELAELAGADPVQFRLRHLDDTRACDLIR
                     AVAEHAGWRAGQAGTRGQPGADGLLRGRGMAYARYIHSRFPGFGAAWSAWVIDLTLDP
                     RDGRIRIARIVVGQDTGMMVNPDGVRHQIHGNVLQSTSRALKEKVEFDSLGVRSREWG
                     AYPILNFDEVPQVDVVLMPRQDEPPMGAGESASVPGPAAIANALFDATGLRWREPPFT
                     PQAMLAGLRAANPAGRVDAAMPVPA"
     gene            complement(21654..22529)
                     /locus_tag="VGC69_18615"
     CDS             complement(21654..22529)
                     /locus_tag="VGC69_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0297347.1"
                     /translation="MRFDLESLRVFAAVVEQGSIAAAAQATHIVASAVSRRVSDLERD
                     AGTPLFERHSRGTRPTPAGQTLYRHARRIFEQFQQAEGELDEYSQGVRGHVRLSVNLT
                     AMVHYLPWALHRFLSANSAIKIDLIEKTSDIVLRTVENGTVDFGICAVTDPVVRLTCV
                     PYRVDRLFLVVPASHRYAGRVSLRFEDVLDEDFVGMQDGASIYTLSQRAAANHGRRLK
                     LRIQVTSFEAVRNMVAAGMGIGLLPEVALSETRSEVVKIPLDELWAERSLQIVYRDFD
                     TLPAAAQLLIEQLTP"
     gene            22751..24163
                     /gene="leuC"
                     /locus_tag="VGC69_18620"
     CDS             22751..24163
                     /gene="leuC"
                     /locus_tag="VGC69_18620"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0297348.1"
                     /translation="MPQTLFEKIWRRHAVRENDDGTTLLYIDRHLVNEVTSPQAFDGL
                     RLAGRRPSQPRSVLAMSDHNVPTTDRSQGIAAIADPVARAQVQALQANCREFGLTEFP
                     LNDVRQGILHVVAPEQGLTLPGTTLICGDSHTSTHGAFAALAFGVGTSDIEHVLATQC
                     LRLPRMKTLRVFIEGELPAGVTAKDVALALLARFGTNFGTGHAIEFCGGTVRAMSMEA
                     RMTLCNLSIEAGARCGMVAVDDITLRYLRERPLAPQGEQWANAEAYWRTLRSDPEAIF
                     DAELALDAAGIRPHVSWGTTPGMSCPVDAAVPDPEREADPVKRAGLRNALAYMDLAPG
                     TRIMDIRLDKVFIGSCTNARIEDLRDAARVVRGRKVASGIVQALVVPGSGLVKRQAEA
                     EGLDNIFRQAGFEWREPGCSMCLGMNEDRLNPGERCASTSNRNFEGRQGRGGRTHLVS
                     PAMAAAAAIAGHFVDVTCFQ"
     gene            24172..24819
                     /gene="leuD"
                     /locus_tag="VGC69_18625"
     CDS             24172..24819
                     /gene="leuD"
                     /locus_tag="VGC69_18625"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0297349.1"
                     /translation="MQAFTTLDRVVVPIDRPNVDTDAILPKQYMKMIGRTGFGLYLFD
                     EWRYLDPAEPGDDCAARRTVPDFPLNQPRYRDAGIMLGRANFGCGSSREHAAWALAEW
                     GIRALVAPSFAEIFRGNCLKNGLLPVVLPDTVIESLFVAVQATPGYHLAIDLSSQTVT
                     EPDGAGHRFDISSFHKAMLLEGLDEIALTLRHACEIDAFTNTRLAQEPWLAAAIR"
     gene            24959..26254
                     /locus_tag="VGC69_18630"
     CDS             24959..26254
                     /locus_tag="VGC69_18630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY0297350.1"
                     /translation="MEIAKNFVGREYCMHQTITEKIIARHARREYVRPGEFVEVSVDQ
                     TWSDDLGSPITLGLLKSNRVPRVFDADRVFVTAMVNAPAHSIETAAVMKDIRGLTSDF
                     DITLYEAGQAGIHNALGIQLGRTLPGELIVGGNSHACMAGALGCFATGMGSTDIAAIL
                     ATGRTWLEVPRTIRYRYTGKLKKWVTGKDLILHTIGLIGVAGADNCAMEFCGEPIEQL
                     SVEERLSMTNMAIEAGAQNAIIAPDVKTFAYVEKIAKRPFEPIYGDPGADVFQEIEIN
                     VDRLEPVISVPFLPSKTTSISQLGEVKLDQVYIGSCTNGWITDLRCAASILKGRKVAD
                     GLRVFVIPSTTQIYKQAIDEGLAQIFIEAGCALSMPTCGPCIGAQMGVLADGERCLST
                     SNRNFPGRQGHVRSETYLCNPAVAAASAIKGFICGPDDL"
     gene            26282..26815
                     /locus_tag="VGC69_18635"
     CDS             26282..26815
                     /locus_tag="VGC69_18635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEY0297351.1"
                     /translation="MWKQGNAWFLGDDVDTDQIMPARYLALKTAEDLAEHALEAVDPS
                     WPTIIKPGDFLIAGANFGCGSSREHAPLGLKGLGVGCIIAKSFARIFYRNSINIGLPL
                     FVLRDDIQHRSQAHRAWVDLSEGQIKFDEAGPVLQGVAPAQIVQDILMHGGLMAYTAA
                     RSGKVQEESKEAGGNAS"
     gene            26871..28232
                     /locus_tag="VGC69_18640"
     CDS             26871..28232
                     /locus_tag="VGC69_18640"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297352.1"
                     /translation="MDSTKATPEIRRAMRITTVSAIVGTAVEWYDYYLYATAAATIIN
                     HLFFPSYDHLTGTLLAYASFAIGFIVRPAGSVIFGRLGDRIGRKAVLYITLLLMGGST
                     TLMGLLPTYASAGLWAPVLLCVLRALQGLGSGAEYAGAVLMVVEYAPAKRRGFYASLP
                     YIGVALGLLLSLASFHLSSVISKDHFEDWGWRIPFLLSAVVVLLGLIIRSRLKETPVF
                     EQIRRDGAVSRAPITEVLRTSLRPMLCAWGARLGDNSLAYIYESFVIVYVTQQLKLPR
                     DLIVTALMWGSVLQCVTIPLFGWMSDLIGRKPVYMLGAIISGIAVFPFFWMLQTKDPL
                     MIYLGLFLVSPVAKIMMTSAQSAWLAEMFPSRVRYTGFALAREVTSPISGGIAPMVAV
                     ALLAAGGGSPHLVAWYVVALGAITTVSVLLGPETRGRSLDETTQDLPHAPIRQAAVSQ
                     NVH"
     gene            complement(28239..29717)
                     /locus_tag="VGC69_18645"
     CDS             complement(28239..29717)
                     /locus_tag="VGC69_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0297353.1"
                     /translation="MTFHLVPRVAALLTVLTGSAVVLAGCAVGPDYHDAPPVAQAALS
                     APAYARAPATAGVVPQAAPSDWWRQLNDATLTALIEAALAQSPDVHAAEARLRQARAQ
                     LGQQRAALTPSLTADVAAPRLRSPNVGSLLGGSSQQSGESQQSGGQGPLQLYSTSFDA
                     SWELDLFGGVRRATESAAAQAEAVSADLADVQVSLAAEVGQAYIGLRDEQQRLRLAQQ
                     SAEVQQKMLDLTRRRRAAGTAADLDVERLTSQVEGTRASLLPLDAQIVESLDRLAVLT
                     GKAPGDLDAQLSPAGDLPVMPASVPVGDPGALLKQRPDIRAAERRLAASYAQIGAKTA
                     DLFPKVTLMGVLGFTSTSFGDVLSKDSFTWMGIPFLQWNFLSFGRTRQAIVQAEAERD
                     EADARYQQTVLSALQDANDALSRYGHQREHVLRLQQVQVSAGRAAQLTRQRYAAGASS
                     LIDLLDAQRVEYSAQQDVVAGQASLLQDYVSLQKSLGLGWRT"
     gene            complement(29736..31310)
                     /locus_tag="VGC69_18650"
     CDS             complement(29736..31310)
                     /locus_tag="VGC69_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY0297354.1"
                     /translation="MVDAGAAHEEPRATVGDWIAVAAGSLGALTATLDISITNSALPQ
                     IQGAIGATGTEGTWISTGYLMSEIVMIPLAAWLTRVFGLRNFLLGNTVLFALFSIMCG
                     MSGSLGQMIAGRIGQGFTGGAMIPTAQTIIRSRLPRSQMPIGMTVFGLTVLLGPLLGP
                     VVGGWLAENISWHWCFLVNMPICGMLAVLLIIGLPTERARWGDFIKADWVGIAGLAMG
                     LSSLTVVLEEGQREHWFDSSMIVTLTIVSVVGMALIALSQFTAERPVMRLSLMRNPSY
                     ASVILIVFAVGAGLYGVSYLIPQFLGLVAGYNAEQAGGIMLVSGIPSFLMMPILPRLL
                     GKMDFRLLVIPGLLMFAVSCWLDLGITAQSVGHDFYGSQFLRGVAQILAMMPLNQASM
                     AAVSREDSGDAAGFFNMARNLGGSVGLALIGILIDRRTTYHVATLRESITANSTLAQD
                     YVANTSASWFARTGDLAYAQLRSLGLLARQIQQQALVMTYIDTYYWLAIALLACIPLA
                     LLLKTPRSGPPKPMEH"
     gene            complement(31303..32307)
                     /locus_tag="VGC69_18655"
     CDS             complement(31303..32307)
                     /locus_tag="VGC69_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY0297355.1"
                     /translation="MLIILAVVAWLAYWLLAGRFIESTDDAYLQADSVTMAPKVSGYV
                     AEVLVGDNQAVKAGDPLVRIDARQYQASFDQANATIAARQADIARSEAEIQQQQANAV
                     QAEAQAHMADIKARHASEEVRRYTPLAAAGAETNERLADLVSERDQALAALAANNAAV
                     NVARTQIQVATTQIAQSRAQLTAAQASARQSELDMQDTLVRATIAGRVGDRSVRVGQF
                     VQPGTRMLTVVPVQDVYLVANFKETQLGRMRPGQPATLSVDALPGVKLHGVVDSFAPG
                     TGSQFALLPPENATGNFTKIVQRVPVRIRVEAGGNADRLLPGLSVTVDVETRQDGNHG
                     "
     gene            32567..33970
                     /locus_tag="VGC69_18660"
     CDS             32567..33970
                     /locus_tag="VGC69_18660"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY0297356.1"
                     /translation="MFRAGQLNLHYSALVVASGLACALCNGLPFFILTQHLGLGEMPD
                     IHWARGLVVMAAFFCFSNFRLLITARHLWWILYRGFFRWIWVLLAAVLGLFYMVHTQE
                     ETERARQLISQWALVALPLQLACLAALRGFIHQINNLAGNRRKAVFFGMSPQASTLYL
                     RLQRSPILGIEVTGYYAEAPVASNFHGLVPPYLGRYETAWPRIQASEFDMVFIQPYIH
                     YGQERSDQIFEQLYNSTSTIYMVPETQWISNVTLSSVDIAGITLLSVHDTPIIGAARQ
                     LKRLMDLVIGGIAFLLLSPVMLAAALAVRLDTPGPILFKQVRYGERGQPFHVYKFRSM
                     YTDTGGPLRQVSQSDPRVTRVGRFLRRTSLDELPQLLNVLLGSMSLVGPRPHAVVHNE
                     LYRQQINGYMLRHSIKPGITGWAQVNGLRGETDTLEKMERRLEYDRYYITHWSLGLDF
                     WILLRTVWTVVHGNNAY"
     gene            complement(33981..>34326)
                     /locus_tag="VGC69_18665"
     CDS             complement(33981..>34326)
                     /locus_tag="VGC69_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY0297357.1"
                     /translation="SASMRYDPEGPEAEEHARRIRELAPQVVFACLGMPKQEKWAFRY
                     GPTLPGGIILCAGMAMRFAIGLQSRAPVWMQRNGLEWLWRMGSEPRRLVRRYLYDDLK
                     FFGLLWRQWRDR"
BASE COUNT         5723 a        11751 c        11212 g         5640 t
ORIGIN      
        1 cccggagccg gcggcgcggt gggcacgcag accctggtgt cttcagcgcc cgacggctat
       61 accctggaac tggccgcgcc ctcgcccttg atcgtggcgc cctacctgat caagaatctc
      121 agctacgacc cgcagaaaga cctcgcgccg atcagcctga tcgccgtggt gccggccgtg
      181 ctgctggtga aggaggactc gcccatccat accgtgcagg acctgctgga catggcccgc
      241 aaggagccgg gcaagctgac gttctcgtct tccggcctgg gcggcaccgc gcatttgtcg
      301 gcgcagatgc tcaaggtgct ggccaacgtc gacatgctgc acgtgccgta caagggcacc
      361 gcgccggcca tcgaggcggt gatgagcggc caggtcacgc tgaccttcgc cgacatgacc
      421 gccgggctgg cctacgccaa gaaccaccag ttgcgggcgg tcgcggtcac cacgcccacg
      481 cgcacgccgg cgctgccgga cgtgcccacg gtcgccgaga cggtgccggg ctatgaggcg
      541 accgtgtggt acggcgtgtt cgcgcccaag ggcacgccgc agcccatcat cgacaagctc
      601 aacagcgaga tcgtcaagtt cgtgcacagc cccgacgtcg acaagatact gctcaacgaa
      661 ggcgcagagc ccaagggttc gacgccggag gagctgcgcg ccttcatgcg cgacgaggcc
      721 gcgcgctggg gcaaggtgct ccaggcctcc ggcgcgcagt agggccggat gttcggcaag
      781 cggctcggtt gcctcagttg cgtatcgtct gcatgaacca ttcgagcggc aggtcgtggc
      841 ccaggccctg cgccttggct ttgcgataga ccaggccgcc caccgaagag aactgcacgc
      901 cccagttgcc cacggtgtag tagaagctga tctgctcgtc gcgggtgcgg ccggccgcct
      961 tgccggagat cacgtcggtg tagacgggcc agtcttcggt gggcaggcgc gacttcttgg
     1021 gctggggcag gcgctgctgc tcttcggccg tgcccgccac gaaggcgccc gggctgcccg
     1081 agatgccctt gcgaaagcgc tcggtctcgg gcatgggcag gtcttcgcgg ccctgctgga
     1141 cggccacgtc gaagcgctgc acgatgtccg ggctgatctc gcgcggcgtc agcaccacca
     1201 cgtgcatgcc gggttcgagc cagtcgggtt ccagcaccgg cgtcatgctg tcggtgcagg
     1261 tggcgacgat gtcggcgccg cgcaccgcgt cgcgcggcgc gtccaccggc acgacctcga
     1321 tgccgtagcg gcgcgaggtt tccgcggcga aggcttcgcg cttgtccttg ctgcggctgt
     1381 agacccgcac gcgctggatg ccgcgcaccg cgaggatcgc ttccaggaag gtgcgcgcca
     1441 tgccgcccga gccgatgatg cccagctcgc gcgagtcggc gcgcgacagc agccgcgtgc
     1501 cgatgcccgc cgcgccgccc acgcgcatgt gctgcaggtg gccgtcgttg agcagcgcca
     1561 gcggctcgcc gttctgcgtg ctgaaaagga acaccaggcc gcagtaggtg cccggctgca
     1621 tgcagtattt ctgctcgctg ccgcccgcgg cgtggtcgcg cggccagacc aggatgtcgg
     1681 acttcaggcg cacggcgagc acgccgccgc tggcgccctc gaccgagccc cagcggtagt
     1741 agccgtcgtc gcgctcgcaa ggcacgtaca tgtccagccg cggccggccg acggcctctc
     1801 cggtaagcaa gccggcgaag gcctgctcct gcgcctcgat gcactcgggc atcgtcagca
     1861 cgtgttcgac cacttcgttg ttgatcagca gcatcgcgtc tccctggttc tctcgtggat
     1921 gcgccgcggc ggcttgtccg gcgccggcgc aagtcatatg gaatactatg gaataccgaa
     1981 tgattttgca tgaggccgga aaaattttcc ctgaatatcg cgtgccagct atccgccccc
     2041 ggaaacgccg aataccccag gaacccgggc cgagccagga aatccagggc gccggggtaa
     2101 actttgccct gaatcgcgcg ggccgccgag gcatggctgc ccgaccggcc cagcgcatcg
     2161 cgggaacgcc caaatgataa aaaaggtcca ggattccaat ctgctgctcg gcgaggccgc
     2221 gtacgaatcc gtgcgcgagg ccatcctggc caatgtcctc aagccgggag agcgggtttc
     2281 cgagtacaag gtcgcggagt ggctgggcat cagccgcacg ccggcgcgcg agggactgcg
     2341 ccgcctcgag aacgagggcc tgctcgcgca gcacccccgg cgcggcctgg tggtggcttc
     2401 catcgacgac gcggccgtcc aggagctcta cgccgtgcgc gagctgctcg aaagcgaggc
     2461 ggccgagcag gccgcgcggc atgccagcgc cgcggaaatc gccacgctca agcacctggt
     2521 cgagatcgaa gcgagcttga gcgagaagcc cgagcagatg tacgagcaca accgcacctt
     2581 ccacgagcag gtctaccgcg cggcgcacaa ccgtttcctg ctcaagttcc tgctgctgac
     2641 cgccgatacg ctgtcgacct accgcaacgt ctccaccctg gttttcaagg agcggcgcga
     2701 cgaggtgctg gccgagcacc gcgagttgtg cgatgccatc gcgcggcgcg acgaggcggc
     2761 ggcccgcgcc gtcgccaccc gccacgtgca gaacgcgctg cgcgcacgca cccgggtgca
     2821 gcacagcaag ctcaccagcc aggcgcagca gcagcgcggg ccggccgacc tggccaagct
     2881 cgaaccataa gcgggatcgc gcaccgctca ggcgggcgcg ggaaagaccg cctggttttc
     2941 caggccggcc agggcctcat accaagccat cgatgcctgg ctccccaggg tgtcttccgt
     3001 cagcaggaca aagcgccggc gcaggctggc ctggtcgggg gggtcttgcg gcatgcccgt
     3061 gaaggaatcg gccagcgcct gcaggcgccg tccgtcatgc agcgcaacgt cgatgcgggc
     3121 gctccaggcc gtcggcagat cctgcgcggc gccaaggtcg atcaatgcgc acatgcgccg
     3181 gatgtcggca tcggccaggg ccgactcgtc gaacgaacgc gggtcctccg gatcgcgatg
     3241 cagggccagc gccacgcaga acggcacact gtactgcgcg gtcatcacgt cgcccggcgc
     3301 gcggatgtcg tggtggctca ccactttttc agacatgcgc agggacaggc ccgccacgtc
     3361 gccggcggca aagccgtgct cgcgcatcat gtcgcgcagg gcctgcacgg cgggctgggc
     3421 cgtgacgtgg caggggtagc gcttcatgca gatcttgcgc gactcccact cccggcccag
     3481 gccggccgta agcagcgcgg cctcgccctc gctgcagtag gtctgcagga atccgaactt
     3541 gccttcgagt atggtctcgg gcccggtata gccttcggac gccagccgcg ccgccacgat
     3601 gccggcctcg caggcgcggc ccatgtgcag gcgcttgacc atggccccct gcccggcctt
     3661 ggtgaaggcc aggagccccg ccgacatcga cccggcgatg cccagcgcgt gcgcgatgcg
     3721 ttcggcgtcc agcccgtaga ccacgccggc cgccaccgtg gcgccgtagg gccccgtcag
     3781 ccccggcgca tgaaagccca gtttttcgct gctgtgatgc gtggccgcgc cgatgcgaaa
     3841 cagcacttcg caggcggcca cgaaggcccg cagggccgtc tcgccatcgg cgccggtttc
     3901 ctggcaggcg gccgcgatgg ccggcaccag cgtggcgccc ggatgcacgc cggccccggg
     3961 aaagcgcagg ctgtcctgct cgaaggcgtg cgccgcggcg ccgttggcca gcgccgcggc
     4021 aggcgcggcc acgccgtgcc ccgcgacgcc gaagacggtg caaggcccca gcccgccata
     4081 gcggcgcgca taggccgccg tggcggcgct ccatggaaaa cgcgcgccgt acaccgagca
     4141 ggccagcgtg tcggcgatgc aggctcgcgc ccgctcgcgc acgtcgtccg gcacgtcgga
     4201 cccgcgcagc cccgccgcgt aagcagccaa ctcctgggaa gccgtcggca tggcgcttcc
     4261 cggatcaggc cttggccagc agccgcggca gttcggcggc gtcggcccgc tggtccatat
     4321 tgagcaccca gtcgcggatg cgttcagcct gcgggcgttg cagcgcgtgc agcgccagct
     4381 tgtcgaactt ctcgacgatg tcggcctcgc tggcgaaatc gcgctcgttg ccgcggccgg
     4441 cctccacggt gcgcgccagg cgcgtgccgt tcttcaggaa gacctcgacc cgcaccatgt
     4501 ggcggaactt ggaacccttg gcggtgatct ccgcgtcgtg cgccacctgc accttcccgg
     4561 ccacgcgcat gcgctcgggg tcggcgacct tgtcttcggt gaactggtcg acgaaacagt
     4621 cgccgtccag cagccaggtg gccacgcaat acggcagatt gagctgcgcg gaagtcagcc
     4681 cctgcggctc gtacttccag cccacgtggt ccatggtgac ctgcgagccg tgcaccacga
     4741 tcttttccac gtcggcctcg ccgaagggat gctcggcctg caaggcgcgg atcgcgtcca
     4801 gcgtgctgtg gttgctgccc acgcaggaat agaacttcag cgcgatgccc atggtctgcc
     4861 acacttcgcc caggcccgcg gtgagttctt ccagcttgaa gcggtcggtc gagcgcgaga
     4921 aggtcgtgca atagccgccg tactcgctct cgagcacatt gacgatgccg gtgaagccct
     4981 gctcggcgaa cagcgcgccg tacaaaccgc tttgcgagga gcggcccgca tgcatgcgct
     5041 tgaccatggc gccgtactgc gcagccatca ggccggccgc ctgcgtgccg gcgatgccca
     5101 gcgcgtgcac ggtgcgatcg acgtccagcc ccaggccgcg cgccgcgccg gccgcggccg
     5161 agaagacccc cagcgtggcg ccggagtgcc agccctgcgc gatgtgctcg ggccccatgc
     5221 agatgcccac ccgcgggccg atctcgtagc cggccaccgc cgacgtgagg aattcgcggc
     5281 cgctcatgcc cggccgcatc tcggcgatgg acaccagggc cggcagcacc acggcgccca
     5341 cgtgcagcac gccctggcgg tgcacgtcgt cgagttcgaa gccctgcacc tgcgtgccgt
     5401 tgaccagcgc cgcgtgcggc gcggacagct tctgcccggt gccccagacc gcgcagccct
     5461 gggtggtgtc gagcccgccc agcgttttct gcaggatgcg gctccattcc agctgcgcgc
     5521 cgtacagggc gcagcccagc gagtcgagca tcaatagctt gatgcgcgtg acgacttcgg
     5581 gggggatggc ttcgtagcgc agcccggaaa cgaactgggc gatgccttgg gtatagcgat
     5641 tgtctgaaag cgtggactgc gacatggggt ctcctctcgg cacagtaatg cttgtggttc
     5701 ttgtgtcatc ccaaaaatag tataccatgg tattctataa acggatgcgc tgccgccgcg
     5761 cctgccaggc acgacaagaa gatgctgcgc ccgctgccac ccggaggaga aaccgtggag
     5821 atacggagac gacaattcat cgcgcggggc ctggccctcg cagccgcgcc cctggcgccg
     5881 cgcgcggccc tgtccgccac ggacgcggac ggctaccctt cgcacccgat gcggctggtc
     5941 attcctttcg ccccgggcgg cggcaccgac atcatcggcc gcgaaatcgc ccaccagctc
     6001 tccctggcct ggaagcagcc ggtcatcgtg gagaaccgcg ccggcggcaa cggcaccatc
     6061 ggcctggcca atgtggccaa ctcgcccgcc gacggctact cgctgtccat ggtcacggcc
     6121 agcgcctcgg tcaacgtcac gctgcagggc cgcaccctgc cctacagcct gatcgacggc
     6181 ttcgcgccca tcacgcaagt gacttcgcag ccctacctgc tggtggtcag ccccgcgctg
     6241 ggcgtcaaga gcgtgcagga actgctggac ctgggccgca agcgcgccac cccgctgacc
     6301 ttcggctcgt cgggcatcgg cggcctgagc cacttgtcgg gcgcgctgtt ttcgtcgctc
     6361 tccaaggtgc ccatgacgca cgtgccctac aagggcggcg cgcccgccat ggccgacgtc
     6421 gccagccggc agatcgacat gctgttctcc acgcaaatcg aggccaacgg cctgatccag
     6481 ggcggcatgc tgcaggcgct ggccgtcacc accgcccggc gcgcggccgc ctcgccgaac
     6541 ctgcccacca tgcaggaagc cggcgtggcc ggctatgaag tggccggctg gtacggcctg
     6601 ctggcgccgg cccatacccc gccggagatc gtggccaagc tcaataccga ggtgaaccgc
     6661 gtgctcacgc tcggcgacgt gcggcagaag atccatgccg acggctccga acccgcgggc
     6721 agcacctcgg cgcaattcgc cgcgcacatc cggtccgaag tcggccgctg gcgcgccctc
     6781 atcgctcaac tgaacatccc cacacaatga ctaccgctac cgaagacgaa accctggcgc
     6841 cacccgtcga cgccgcgccc ggcctggcgc gcgaactggg cgcccgcgtg gccggcctgt
     6901 cgttcgagcg cctgtccgcg gacgccctgc gctgggcccg catgggcatc ctcgacacca
     6961 tcggcgtgac cttggccggc gcccgcgaag acgcgccgcg cctggcggcg cgcgcactcg
     7021 acctgggcga gggtccctcc ctggtgctgg gcgcgcgccg gcgcgtgggc gcgctcgatg
     7081 ccgcgctgat caacggcacc gcgtcgcacg cgctggactt cgacgactgc aacaacaccc
     7141 tgggcggcca cccctcggcc cccgtgctgt cggcgctgat cccgctggcc gaacagctcg
     7201 gcagctcagg acgcgacttc gtgaccgcct acgtggccgg cttcgaggcc gagaccaaga
     7261 tcgggctggc ggtgaacttc caccactaca ccaagggctg gcaccccacc gccacgctgg
     7321 gcaccttcgg cgccgccgcc gcctgcgccc ggctgatggg gctggacgcg gccggcaccg
     7381 ccacggcgct ggcgctggcc gcctcgttcg cctcgggcat caaggccaac ttcggcacca
     7441 tgaccaagcc gctgcacgtc ggccactgcg cccgcaacgg cctgtacgcc gcccggctgg
     7501 cgcgcgaggg cttcaccgcc aacgccgcgc gcgtgttcga gcacaagcag ggcttcctgg
     7561 acgttttcaa cgggccgggc acctacgaca cgcagcgcgc cctcgacgcc tgggccaagc
     7621 cgctggacat cgtcgagccc ggcatcgccg tcaagcaata cccttgctgc ggcagcaccc
     7681 accccgcgct cgatgccatg ctggaactgg tgcggcggca cggcccccgg ctcgagcagg
     7741 tcgagcgcgt ggacgcctgg atacacgcgc gccgcctggc ccataccaac cggccgcggc
     7801 ccaacagccc gctggatgcc aagttcagcc tgcaatatgt gctggcgcgc gcattgctcg
     7861 acggccatgt cggcgtgaac gatttcatcg agagcgccta ccggcagccc gaagtgctgg
     7921 cgctgctgcc gcgcatccac gtggcggcct acaacgagac cggcgtgggc ggcttccccg
     7981 aggccaatca cttcggcggg ctggtgcgca tcactctgcg cgacggcagc gtgctcgaag
     8041 cccaggtgga ccagccgctg ggccgcacct cggccaaccc cttgccgccc gccctgctgc
     8101 gcgacaagtt cagcctgtgc gcctcggccg tgctggcgcc gccggccatc cccgccatcg
     8161 ctgaaaccat cgagcgcatc gagaccctga acagcgtcgg cgaactcacg gcgcagatcc
     8221 atcgcgccac gacctgaccg aacaccccaa gacccagagg acacgcaatt ggaacagcct
     8281 actcaccact atgacgtcat cgtcgtcggc aagggcaatg ccgccctgtg cgccgccctg
     8341 tcggcccagg aaaacggcgc gcgcgtcgcc gtgctggaag ccgcatcgca ggacgaatcc
     8401 ggcggcaaca gccgtttcgc cggcggagtc atgcgctttg cctatgcgtc ggtggacgac
     8461 ctcaagaccg tcaccgagct cacggaagaa gagatcgcca ccagcgacta cttcaccaac
     8521 accaccgacg aattcttcga cgacctgttc cgcctgacca gcttccgcac cgatccgcag
     8581 ctctcggaaa tcctcgtgac ccgcagcctc gaaaccatgc agtggctgcg cggcaagggg
     8641 gtcaagttcg tgcccaatta cggccggcag tcctcgctgg tgaacgggcg gcgcaaattc
     8701 ttcggccggc tgcccatcga ggtgtccggc ggcggcgccg ggctggtggc attcctcgac
     8761 cgcgccgcgc agaaagcggg catcgacgtg cactacaaca cccgcgtcca gcagctcgtc
     8821 ctggacggcg agcgcgtggc cggcgtgctg gccaaccagg agggcgaaca ggtggaattc
     8881 ctcggcaagg ccgtggtgct ggcctgcggc ggcttcgagg ccaaccccga gatgcgcgcg
     8941 cgctacctgg ggccgggctg ggaactcgcc aaagtgcgcg gcacgcgctt caaccagggc
     9001 gacgggctgc gcatggcgct cggctccggc gcggcctcgc acggcaactg gtcgggctgc
     9061 cacgccaccg gctgggacct caacgccccc gagttcggcg acgtcgacgt cggcgaccag
     9121 ttccagaagc acagctacat cttcggcctg ctcatcaacg cccacggcaa gcgcttcgtc
     9181 gacgaaggcc tggacttcca ctcgttcacc tacgccaagt acggcggcga agtgctcaag
     9241 cagccgggcc agttcgcctg gcaggtgttc gacgacaagg tcaccaagct gctgcgctcc
     9301 gagtaccgca tcaagatgat caccaaggcc acggccgaca cgctggaaga cctggcccag
     9361 aagctggagg gcgtggaccc ggaggccttc ctgcagaccg tgcgcgccta caacgccgcc
     9421 gtcaaggccg acgtgccctt cgaccacacc atcaaggacg gccgcggcac cgtgggcatc
     9481 gagccgccca agtccaactg ggcccaggcg ctggacacgc cgccctacca tgcctacgcc
     9541 accacctgcg gcatcacctt cacgttcggc ggactgcgca tcgacccgga caacggccag
     9601 gtgctggacg tgcacctgac gcccatgccg gggctgtatt gcgcgggcga gatggtgggg
     9661 ggattgttct acttcaacta tccgagcggc acgggattgg tgtctggcgc ggtattcggc
     9721 cgcatcgcgg gcgcgggcgc cgcgaaagaa gccgccgcgg gttgacggcc gcggcgacta
     9781 ggctcaagcc gcccctgttc gcgcaggggc ggctttttca tttcggggca accggcacgg
     9841 gtcctggccc ccgaaagcct gtcgatgctg tgcgggataa gcgcagtccg gttcgacgac
     9901 ccattatgat gcggggcggc gaaccggaaa tcgccgcgcc gtccgcagcc ctatccttgc
     9961 tcatgactga aaccctcgcc attcaacctg aagccatgcc ggcatcccgg cgccgctggg
    10021 cgatgttctg cgtcctgctg gccctggcgc tcagctccct cgacagcgcc atcgcgaata
    10081 ttgccctgcc gacgatcgcc cgcacgttgg cgacgagcaa ttccaactcg atctggatag
    10141 tcaacgccta ccagctttcg gtcatggtgt gcctgctgcc cgtcgcggcg ctcggagaat
    10201 cgctgggact gcggcgtatc tatgcggcag gcctgaccat cttcaccctg gcctccttgt
    10261 gctgcgctct gtcgccgacg atcacggtgc tggtctgctc ccgcctggcg caaggcgtgg
    10321 gcggcgcctg catgtcggta tcgtccgtgg cgctggtgcg cggcatctac ccacgcgaaa
    10381 cggtccgcaa tggctatgcg ctcgtcgcgc tggcggtggc cgcctcgggc gcgctgggcc
    10441 ccacgatcgc cgccctgatc ctctcggtcg caagctggcc ctggctgttc ctggtgaatt
    10501 tgcccttcgg catcattgcc gtgccgctgc ttctcgcggt ggtaccgccc gatgtccgcc
    10561 atccccgccc gttcgactgg atcggcgccg gactcaacgc cctggcattc ggacttgtca
    10621 tcagcggcgt gcactccctg ggcgatgaaa gcatcggtgt cgccgtggcg atgatcgtcg
    10681 ccgggattgc ctgcctcggc ctgctcgccc gccagcaaat gcggcagccg cagccgctgc
    10741 tgcccctcga tctgctgcgc atcccgcagt tcgcgctatc ggtggcggca tcggtctgca
    10801 cctatgcggc gcagatcctg gcctatgtct ccattccctt tctgttcgag gtgaatctgc
    10861 acctgacacc ggtcgaaaca ggcctgcttg tcacgccatg gcccttgctg gtcgctttca
    10921 cggcgcccct tgccggacgc ctgatgtctc gccatccggc cgatctgatc gcgagcgtcg
    10981 gtttgctcgt gctggcctgc ggcctggtcc tgatggcggc gctgccggcc gagcccgcct
    11041 atgggaacat cgtgtggcgc ctcggcgttt gcggtattgg ctttggactt ttccagacgc
    11101 ccaacaacac cctcatgatg acgacgggcc ccgccaagcg cagcagcgcg gccagcggcc
    11161 tgaacgccgt cgcgcgcttc atgggcatgg ccttgggatc ggccgtggct gccgtgatcc
    11221 tcggggtggg cgggcagcat gcgacgatcg catgcctgga agtcggcgca gcctttgccc
    11281 tggtcggcgc cgccgccagc ggcgcgcggc gcaaatggca aggccagggc ctatcgtgat
    11341 tgccgacgcc tagcggtggg ccgcgatata gatcttgagg acgatagtcc cgagcaccat
    11401 cgccatgccc aggggcatga gcaccgggcc ggccttgtag tcgtgaagat tgaagccgac
    11461 caggagaagc acgatgccta tgccgccgat gacgggtatt agatccagat cgaagaggct
    11521 catggcttga gtatgggata gctatcgggg gacggcgcca agcggctggc gccaaggatg
    11581 cgtgcggctt gcaagaggcc attctatccg cctgggtgtc aattcaaaaa atttgttgat
    11641 tcaggccgag agaagcagcg cctggatctc ctgcctcggc agcttgccgg ccagcgccag
    11701 catcagcagg atgcgcagct tgcccggcga caggccgccg cccgacagga tgccgtcggc
    11761 cgtgttgtag gcctgcagcg gcacggcgcc gcgcagggcg cgcgaggcct gcaccaccgt
    11821 gacgccctgc ctgaccgcgc gcagcagcgc cgcgcgctcg gccggcgtgc aacggcccgg
    11881 cgggaagccc acgctgatga ggcccgcgca acccgccgcc gcataggcgt cgatggccac
    11941 gccgtccgcg ccggcatacg aataggccac gtcgacgcgc gcaaagtcct gggccgacca
    12001 ggccgagcca tcgaacacgc gcgccggcgg cagggcgccg cggcgcatgg acaacatgcc
    12061 gctggcctcg atgcggccca gcggaccgaa ctccggcgcg tcgaaggcat cgagcgcgtg
    12121 attggcggtc ttggaaacgt cggccgggct gtacaggtag ccgttcatgg ccacgtagac
    12181 gccgaccggc gcctgggccg cggcggccag cgcattgcgc acgctgctga cggcatcgct
    12241 ggacaccgtg ttgggcggcc gctgcgaacc gcagacgacg accggcatgc cacagcgcac
    12301 cgtgcaatgc aggaagaaag cggtctcttc cagggtggcg gtgccgtgcg tgacgacgat
    12361 gccgtccacc tcgccgctcg cggccacggc ctggatctcg cgggccagat ccagccagtc
    12421 cttgggggaa atgccggtgc ccggcagggc ccggaacgga tgcgacacga cgtcgacgaa
    12481 cggcaggccg aaactcatgc cggcgaccac ctcatccact gcgtggatga tgccgctgtc
    12541 gccgtagtcg acccaatcga aagaatgggc cccttccatg gcaatggtgc cgccggcacc
    12601 gatcaccgcg atcctgcggc tcacagccgc accttcacca cgtcgttctc gaccaggatc
    12661 gggtacaccc gggtcggcct ctcggcgggc gcgcccttga cgctgccgtc gcagtacgag
    12721 aacaggccgc cgtgcagggg gcactcgatg acgccctctt ccggatcgca gaaaccgtcg
    12781 gacaggaacg ccatgccgtg cgtgcacacg tcgtcgagcg cgtagtagcc cttgccgtcg
    12841 ttgaccacgc acaggcgctt gccgttgaca tggaaggcct tggcatcgcc ttcgtcgacc
    12901 tcgcctgcct ggcagacgtc ggaccattgt tgggtatcgc tcatgcagag cctcttggaa
    12961 tatgttgtga aagatgacgg aaaagccgga agcgccgcca ggcccgggtc aaagatcgaa
    13021 gggctcgcct tcggggatca ggcagcgcag ttgctccggc ctgccagcca gttcgagggc
    13081 atgcagcgag gcgaattgca tgcgggcgat gcgccagcct tcgtcgtcgg cggcgcaatc
    13141 ctggtcgacc acgcccgtca gcagggcgac cttgccgtcc tgctcgcgga tgccgagctc
    13201 gagcaggcgc cagcgcacgg cggccgaccg gcccgatgcg tcgatgcgca tgatgggatg
    13261 gccatagtga tgcgcggtga acagccaggg gctggtctcg aaaaacgcgg cgatggcctg
    13321 ccggccgacg aggtcgccgc cgaaacggcc gcccgcccag cgggcatcgg ccgtaaagca
    13381 ttcggcctgc tcgcgcgccg ccgcgcggac gatgtccgcg gactgcttct ggcgcaatgc
    13441 cgtgtacttg cgatcggcgg cggcggcgta gcgctgcatc agttcgtcga tctgctcgcg
    13501 cgcttcgagg cgcgcgagcc gccgggccag gtctgccgat gggtccatgt tcgtgccttg
    13561 cttgagttcg actacgttcg actacttgac cttgacgcgg tagaccacgg cttcgccggg
    13621 cacttcccag tgcaggggca tgccggcggg caccagcagg cccaggccgg cgcgcagcgc
    13681 gtagtcttcg tcgtcgcaga ccaggcgcgc ctcgccgtcg agcacggcga tcatttcctc
    13741 ggcgccgggc gcttcgtcga aggtgccgcg gatgcgctcc atgcgggcgg ccaggcgctc
    13801 gccctcgatg ccgaagggcg ccttgcccat gccgctctgt tcaacccggc acaaaacgct
    13861 catttcgatg ctcccttctc gtcggcgctg gggtaataca ccttggaccg gcccttgctg
    13921 ccgtactcgg cgcgcggcgg ggtcacgatg ttcaggcgca ggctccagcc ctgggcatcg
    13981 aaatgattga tgccggaatg cgcctgctcg gccggcatga ccatgaccga gccctcttca
    14041 agcagcacgg aaccctccgg ttcggtgacg ctgtggccca ggttgaccgt gcagccgccg
    14101 cggatctgca gcgtgcattc ttcgccgtgg gcgtgcgact tgggctcgat gcccgggccc
    14161 gcgggcaggc ggaaattgat gacgcccacg ctcatggtct cgcccaccac ggactgcatg
    14221 tacagcccgc cggggaccac ctcgcgcagg tcttcgctga aagaaaacaa ttggcacttg
    14281 gacatggcgc gcgacctcag atggcgggac gcagcgtgtg ccgctcgtcg gccgcgaaca
    14341 ggcgcgagtc gtagccgtcg gtcaccacga cgctggggtc gtaggactcg atggccggcg
    14401 ccacctcggc cagcgacagc agcatatgga acaggttcag cgtctgctca tcgtacttgc
    14461 cgtcgacctg gtgcgcgtcg atccaccggc acgccgcgtc gaccgcgggc agcatggcgt
    14521 cggcaaaggc cttgatctcg ggaaacttca ggctcacgcg gcggtggttg cgctcggtct
    14581 cggtggccag gcaccagtcg ttccagggtt ccaggtagcc gaaggattcg ggcagtttat
    14641 tgctcatggc gatgcgctcc gtatcaggcg gcctggccgt cgcggcgcag gccttcctgg
    14701 atgatcttgt cgatgacgac ggcctggtgc cgcaccatca attcgtagtc ctggatgatc
    14761 atttcactct tggcgccggt ctcggcggcc ttttgcgtca tttccaatgt gctgaggtct
    14821 tcgagcagca catcgcgcag ggcgaccttc atgtactcct ggtagaagcg gccgccggcc
    14881 gtcttcattt ccgggtagta gacgcgcgcc tcgaacagga acttgttgtg cgtgagggga
    14941 cggaagttgt aggcctggaa gtagttgggg cggcacgaca ggtagaagtc ggggaagacc
    15001 acattgatgt cgaaggccca gtacggatgc ctagtccagt tgagcagctc gggcagcttg
    15061 acgtcgctga agtccagcct ggtgccgttg gcggccaccg ccagcgcctt ggcggcgatg
    15121 gcgcggccgg agccctggct ggcctgcgcg gcgtcggtcg cggcggcgcc ttgcgtggcg
    15181 gcctgcgggt tgccgtacac cgtgttggga ttgcccgcga tggacaggcg gcggtgcagg
    15241 tcgccgcaca cgccgtcgat gggatgcagc atgccctctt cgtcctggtc gagcgcgtag
    15301 gcgatcgagc ggccatgcac atagcccacg tgataggttt cctggaaggc gtcgagcgcg
    15361 accttccagt tgcaatcgac cacggcgttc caggcgtagc aggcggtgaa gcgctcgaag
    15421 ggatagcttt cgaagcccgc gtacatgggc tccatgaagt tcttcaggct caccgagggg
    15481 ttcggatcga ggttgatgaa caagaagccg ttccaggtgt cgaccgacac ctgggtcagg
    15541 ccgtagcggc tcttgtcgag accgtcgatg aacgattttt cgtcgggcac gccgctcagg
    15601 ctgccgtcga ggtcgtaggc ccagccgtgg aacttgcaga agaacttgcg cgtgttgccg
    15661 gccggctcgt aggcgatgcg gttcaggcga tgcgagcaga cgttgtggaa ggcgtggatg
    15721 cccttgctgc ggtcgcgcgc gatgacgatg gacgaatcca gcgcggggat ttcgcgcacg
    15781 atgtagtcgc ccaccttggg gatttcctcg acccggccca cgtgcagcca ggttttcttg
    15841 aagaggtatt tcttctcgag ctcgaagtag gccggatcgg tatagggcgc taccggcagg
    15901 gggccgcggc cgagctcggg gtagcgatcg tggagtcggg caggttgggc ttgcatgatg
    15961 tgtcctccgc agttaggatt tagtgtatac gttatacgta atacgttaac ctagggttat
    16021 tactgaaccc gccagcattg ccaagcccgc ccaaaggagc accgcccatg tcccagacta
    16081 tcgtccgcgt ggcagatctg cgccagcagg tctatgacgc gctcaaggcg cgcatcacca
    16141 cggggaaatt cccgtcggac gcccacctgt tcgagatcag cctggccaag gaactgaacg
    16201 tgtcccgcac cccggtgcgc gaggcgctgg ccatgctggt gcgcgacgga ctgctggtgc
    16261 agctggcgcg cggctttcgc ttcccccggt tttccgagca ggagatcatc gagatcatcg
    16321 agatccgcct gctgctcgag ccctatgcca tcgagcgcat gatcctggac aacactcgcg
    16381 ccgccatggc cgccctgggc gccggcatgc gccgggacct gctggccgcc ggcggcggcg
    16441 acgcccgggc ctatgccgag gtgcaccgca aggtgcgcga cgacatctac gcccacgtcg
    16501 gcaaccggca gttggccgac gccatcgcca agtacgagga ttcgatccac tacatccgcg
    16561 tcagcacgct caacgaggaa gaatcgcgcc aggtgtccta cgacggcatg ctgcgcctgg
    16621 ccgacgccat cgcccgcggc gaggccggca cggcgcgcaa gctcatggag cagcaattga
    16681 tcaatgcccg cctggccttc ctggaccacg tgaccgccgc agccgagctg gaggcgcaat
    16741 gagcctggca gacaaggcat atggttatcg cgcccgcgtc gggcagctct acccttcggg
    16801 cgggctgtgc gatttcgaaa tgcagcgcat ggcgccggaa ggcgtgcagt tcgtcaccac
    16861 ccgcatgccc tttcgcgaca ccagcaagga gtccgacctg gcgctggtgc gggacctgga
    16921 aaacaatgcg cgcctgctgg ccgacgccca ggtgggactg atcgccttca actgcacggc
    16981 ggccagcctg ctgtgcgggc cggccgtcat ccgcgagcgc atccgcgccg ccacgggcat
    17041 agactcgatc accaccatcg aagccgtgga agacgccttg cgccgcttct cggcgcgccg
    17101 catcggcctg ttcacgccct atcgccgcga agtcatagaa agcgagaagg aatacctcgt
    17161 gcagcagggc tacgaaatct gcgccgaagc gcacatcgaa tgcctggacc cggtgcagca
    17221 aggttctatc ccgccggcca cctggctgga cctggcgcgc cggaccgacg tgagcggctg
    17281 cgacacgctg ctgttcagct gcgcggggat ttatctctcg ccggtgctgg ccgggatcga
    17341 acaggccacg ggcaagccgg tcgtgtcgag caatgccgcg ctgctctggc gcatattgcg
    17401 cgagctgggc atcgccggcg acccagcgca cgggcgcctg ttcgccgcgt agcgacgtcg
    17461 cgccgctacc gtcgcgccct tacgcccggt tcttacgccg cggcgtccgg cgcggcggca
    17521 tcgcacatct tgcgcaagag gtagacgatg gccacgcgct cggccggatt gagcgcgccg
    17581 aaggtgcttt cggtaatttc ctgggcaaag ggcaccgtct tgcgcaccag cgccgtcccg
    17641 gcggcggtca gcgtcaccag caccttgcgc ttgtcctgcg gatcgtgcgc cacgctgatg
    17701 agcttcttga ccttgaggcg atcgatcacg ccgcgcaccg tggcctggtc gatcgcggtg
    17761 tgcttgacga tctcggccag cgagcaggcg tcctgctcgt gcaccgcgca cagcaccacg
    17821 aactgggcgg aggtcagctg cgtatcgggg atgcgctgct ggaagatcgc cacgtggcgc
    17881 tggtaggcgc gccgcagcag gtggcccacc tgctccgata ccaggtatgc gtcggcggcg
    17941 gcgtcgggtt tcatcagccc ttgagcggtt cggagaacac gtcgtagccg aagcaccatg
    18001 cgggatcttc gttggtgcgc agccaggtgt tgttgtgcga gacgaactgc accttgctgg
    18061 cgcgttcgat gcggttgtcc gcgtacagct tgaaggccgc ctcgtagtcc gtgaagccga
    18121 cctgctcgaa cacgcgcgtg agcatggcgg cgtcctcgat ggccatggcc gccccttgcg
    18181 ccatgtgcgg cttcatgggg tggcaggcgt cgcccagcag cacgatgcgg ccgcggctcc
    18241 acaggggcag cgggtcgcgc tcgaggaacg accacttgga gatttccacc gtgttctcga
    18301 tcagcgcctg cacggtcgag tgccagccgg cgaagacctc gcgcatttct tccttgctgc
    18361 tgggcacgaa gcggtcgccc gcgtcccagc cctcttcggg cacgccggtg acgtagtaga
    18421 tctcgtcgcg cttgccggtg aggtagtaga ccatcatgtg gcggtcgtcg gcccaccact
    18481 tgcagcattt gtcgaaaggc agggcgccgc tgtcgactgg cgtgggaaag acggcgcgat
    18541 gcccgacgta gccggcatac ttgggctgct cggggcccag cagatattcg cgcagcttgg
    18601 aatagacgcc gtcggcgccg atggccaggt cggcctcttc ccagctgccg tccgcgaagc
    18661 ccaggcgcac cacgctgccg gtgtcttcca catgggtgag ctgtttgccg aaagcgatcg
    18721 cgtccgaagg gatcgccttg acgagcagat cgtggaaatc gccgcggtgc acggtgaggt
    18781 aggccgcgcc gtagcgctcc accgcatagt cgcccaaggg gatgcgggcc atgacctcgc
    18841 cggtcttgcc gtcgcggctg taccaggcct cggggtgcga gccctgctcg ctgagcgcgt
    18901 cctcgatgcc gatgcggcgc aggaccttca tgacgttggg accgacgtgg atgcccgcgc
    18961 ccagccgggc aaaagccggc gcctgctcgt agacgcgcac gttgaagccg gcgcgggcca
    19021 tcagcccggc gcatgccgcg ccgcccaagc cggcgccgat gatggcgatt ctgggtttgc
    19081 cttgcacgat tgttctcccg aacctgtagt ttcccagcct ggagctggca tgtttcacat
    19141 caaaatagag tgtatacgct cattttttgc aagccaaccg tcttcttcgg tttttcggga
    19201 atacccctat gaagcaacat acgaatccct cttgccaagc ctggccgcgg actgcaagaa
    19261 ttaagcgcac acattctatt ttccggccat gaacctgcct gcccccaggg ccttgcacca
    19321 ggacctgctg agcgccccca atacgctgct ggtcgtccgc gaccccatgc ccgccgccaa
    19381 gccggcgccg ggccagccgg gtttcgcctc cgactatgtg cccgatacgc cggacatctt
    19441 catcgccgtc ctgcccgacg gccgggtgca ggccttcaac ggccacgtcg atctcggcac
    19501 cggcatccgc accgccctgg cccagctcgc ggccgaagag ctcgacgtgg ccttcggccg
    19561 ggtcgagatg gtgctgggcc ataccgaagc cgcccccaac caggggccca ccatcgccag
    19621 cgccagcatc cagatctcgg ccgtgcccct gcagcgcgcc gccgcgcagg cacgcgcctg
    19681 gctgctggcg cgcgccgccg ccctgctgga cgccgacccc ggcgcgctca ccgtcgacga
    19741 tgcccggatc agcgcgccca acgggcgcag caccgactac tggcaagtgc tcgcccaggg
    19801 cggcggccgg atcatgctgg ccgcccccgc cgaccccgtc gcgctcaagc cggccagcca
    19861 gcacaagctc atcggccgcg gcgtcgcccg cgtggacatc cccgccaagg cgacgggcgg
    19921 gctcaccttc gtgcacgacg tgcgcctgcc cggcatgctg cacggccgcg tggtgcgccc
    19981 gccctacacc ggccgcgacg ccggcgattt cgtcggccgc agcctgcgcc gggtcgacga
    20041 ggcgtccatc gcgcacctgc cgggcgtggt gaaggtcgtc gtgcggggcg acttcgtcgg
    20101 cgtggtggcc gaacgcgaag aacaagccat ccgcgccgcc cgcgagctgc gcgtggagtg
    20161 gaccacgccg cccgccgccc ccgcgctcgg caatctggac gaggcgctgc tggccatccc
    20221 cgccgtgcgc cggcaactca agaacgaagg cgacatcgag gccaccctgg ccgatccggc
    20281 catgaaggcc ctgtcggcca cctatgtctg gccctaccag atgcacgcct ccatcggccc
    20341 ctcctgctcc gtggccgatt ggcgggacga acggctgacc gtatggtccg gcacgcagaa
    20401 cccgcacatg ctgcgcatcg acctgcatcg cctgctggac ctgggcgaag accgcatcga
    20461 gatcgtccgc atggaggtcg ccggctgcta cggccgcaac tgcgccgacg atgtctgcgc
    20521 cgacgccgcc ctgctggccc gcgccgtggg ccggcccgtg cgcgtgcagc tcagccgcgc
    20581 ccaggaacac acctgggagc ccaagggcac cgcgcagcgc atgcaggtca gcggcgcggt
    20641 cgacgcgcaa ggcaatctgc aagcctacga cttcgtcaac cgctatccat cgaacgacgc
    20701 gcccaccctg gccctgctgc tcaccggcgt cgtcgacccc gcgccgcgcc ggatggaaat
    20761 gggcgaccgg acctccatgc cgccctaccg ctacccgaac ctgcgcgtgg cctgcgacga
    20821 cgtcccggcc atcgtgcgcg cgtcatggct gcgcggcgtc tcggccctgc ccaattcgtt
    20881 cgcccacgac tgccatatcg acgagctggc cgagctggcc ggcgccgatc cggtgcagtt
    20941 tcgcctgcgc cacctggacg acacccgtgc ctgcgacctg atccgcgccg tggccgaaca
    21001 cgccggctgg cgcgcgggcc aggccggcac gcgcggacaa cccggcgccg acgggctgct
    21061 gcgcggccgc ggcatggcct atgcccgcta catccacagc cgcttccccg gtttcggcgc
    21121 ggcctggtcc gcctgggtga tagacctcac gctggacccg cgcgacggcc gcatccgcat
    21181 cgcacgcatc gtggtcggcc aggacaccgg catgatggtc aaccccgacg gcgtgcgcca
    21241 ccagatccac ggcaatgtgc tgcagtccac cagccgcgca ctcaaggaaa aagtggagtt
    21301 cgacagcctg ggcgtgcgca gccgcgaatg gggcgcctac cccatcctga atttcgacga
    21361 agtgccgcaa gtcgacgtgg tgctcatgcc gcgccaggac gagccgccca tgggcgcggg
    21421 cgaatccgcc tcggttcccg gcccggcggc catcgccaat gcgctcttcg acgccaccgg
    21481 gctgcgctgg cgcgaaccgc cgttcacgcc ccaggccatg ctggccggcc tgcgcgcggc
    21541 gaacccggcg ggccgggtcg acgcggcgat gcccgtgccc gcctgaacac aggcaagggc
    21601 ccacacctgc cacggatgac tgccgccgag cagggcaacc atggaagcgg aactcatggg
    21661 gtcaattgct cgatcaacaa ctgtgccgcg gccggcaaag tgtcaaagtc ccgatagacg
    21721 atttgtagcg aacgttccgc ccagagttcg tccagcggga ttttgaccac ttctgatcgt
    21781 gtttcggaaa gggccacttc cggcaacagg ccgataccca tgcccgcggc caccatattg
    21841 cgcacggctt cgaagctggt gacctggatg cgcagcttca ggcgcctgcc gtgattggcc
    21901 gcggcgcgct ggctcagcgt atagatcgag gcgccatcct gcatgccgac aaagtcttcg
    21961 tccaacacgt cctcaaagcg cagactgacg cggccggcat agcggtggct cgccggcaca
    22021 acgagaaaca gcctgtccac gcggtaggga acacaggtca ggcgcaccac aggatcggtc
    22081 accgcgcaga tgccgaaatc caccgtgccg ttctccaccg tgcgcagcac gatgtcgctg
    22141 gttttttcga tcaggtcgat cttgatggcg ctgttagccg acaagaaacg gtgcagcgcc
    22201 catggcaagt aatggaccat ggcagtgaga ttgaccgaca gccgcacgtg gccacgcacg
    22261 ccttgggagt attcgtccaa ctcgccttcg gcctgctgga actgctcgaa gatgcggcgg
    22321 gcgtggcggt acagcgtctg gccagccggc gtgggccgtg tgccccggct atggcgttcg
    22381 aagagcggcg tgccggcatc gcgctccagg tcggagaccc ggcgactgac cgccgaggcg
    22441 acgatatgtg tggcctgcgc cgcagcggcg atgctgcctt gctccacgac cgcggcgaat
    22501 acccgcaggg attcaagatc gaaacgcatg gccctgcctc ctggcctagg cggccatagc
    22561 catctctttt tgcgatggct gggttgtcga aaaagaaata gcggtcagca ggaccgagcg
    22621 ataggatgct cggatactct ctaggcagag gtctttttta tgctgaaacc ccggcactga
    22681 ctcctgttta gcgacgatca tagcccggct ttcaggagac aagctgccgg gcaacacttg
    22741 ccgattcgcg atgcctcaga ctctattcga aaaaatctgg cgccgacatg ctgtccggga
    22801 gaacgacgac ggcaccacgc tgctctacat cgatcgccat ctggtcaacg aagtgaccag
    22861 cccgcaggct ttcgacggcc tgcgcctggc cgggcgcagg ccgagccagc cgcgctcggt
    22921 actggccatg tcggaccata acgtcccgac cacggaccgc agccagggaa tcgcggccat
    22981 tgccgatccc gtggcacgcg cccaggtgca ggcactgcag gccaattgtc gcgagttcgg
    23041 cctgaccgag tttcctctca acgatgtccg gcaaggcatc ctgcatgtgg tcgcgcccga
    23101 gcaagggctg accctgccgg gcaccacgct gatctgcggc gactcgcaca ccagcacgca
    23161 tggcgccttc gcggcgctgg ccttcggcgt gggcacgtcc gacatcgagc acgtgctggc
    23221 cacgcaatgc ttgcgcctgc cgcgcatgaa gaccctgcgg gtcttcatcg agggcgagct
    23281 gcccgcgggc gtcaccgcca aggatgtagc gctggccctg ctggcacgct tcggcacgaa
    23341 tttcggcacc gggcacgcga tcgagttctg cggcggcacg gtgcgcgcca tgagcatgga
    23401 agcccgcatg acgctgtgca acttgtccat cgaggcgggc gcgcgttgcg gtatggtggc
    23461 cgtggacgac atcacgctgc gctacctgcg cgagcgcccc ctcgcgccac agggcgagca
    23521 atgggcaaac gccgaggctt actggcgcac gctgcgcagc gaccccgaag cgatcttcga
    23581 cgccgagctg gcgctggacg cagcaggcat ccgcccccat gtgtcctggg gcaccacgcc
    23641 cggcatgagc tgcccggtcg acgcagcggt gcccgacccc gagcgggaag ccgatccggt
    23701 caagcgtgct ggcttgcgca atgcgctggc ctatatggac ctggcgcccg gcacgcggat
    23761 catggacatc cgattggaca aagtattcat cggttcctgc accaatgctc gcatcgagga
    23821 tttgcgagat gcggcccgcg tggtgcgcgg ccgcaaggtg gcctcaggca tcgtgcaggc
    23881 gctggtggtg cccggctcgg gcctggtcaa gcgccaagcc gaggccgagg ggctggacaa
    23941 tatctttcgc caggcgggtt tcgaatggcg cgaaccgggc tgctcgatgt gcctaggcat
    24001 gaacgaagat cgcctcaacc ccggcgagcg ttgcgcttcg acctccaatc gcaatttcga
    24061 gggccgccag ggccgcggcg ggcgcacgca tctggtcagc ccagccatgg ccgccgccgc
    24121 cgcgatcgcc gggcatttcg tcgacgttac ctgtttccaa taggcccgga catgcaagcc
    24181 ttcactacgc tcgaccgggt ggtcgtgccc atcgatcgac ccaatgtcga tacggacgcc
    24241 atcctgccca agcagtacat gaaaatgata ggccgcaccg gttttggcct ctatctgttc
    24301 gacgaatggc gctacctcga cccggccgaa cccggcgacg actgcgccgc gcgccgcacc
    24361 gtgcctgact tcccgctcaa tcagccacgc taccgggatg ccggcattat gttgggacgc
    24421 gccaattttg gctgcggctc cagtcgcgaa cacgccgcct gggcacttgc cgaatggggg
    24481 attcgagcac tggtcgcacc aagttttgct gaaatttttc gcggcaactg cctcaagaat
    24541 ggactgctcc cggttgtatt gccggatacg gtgatcgaga gcttgtttgt cgcggtacaa
    24601 gcgacgcccg gttaccactt ggccatcgat ctatccagcc aaaccgtgac ggagcccgac
    24661 ggcgccggcc atcgcttcga catatcgtcc tttcacaagg ccatgctact cgaaggcctg
    24721 gacgaaatcg ccctgacgct tcggcatgcc tgcgagatcg atgcattcac caataccagg
    24781 ttggcgcagg aaccatggct tgccgcagcc atcaggtaga agaaattcgt catcgacact
    24841 catgccggac ccgattctgt tttgatccgg ttgcaatagt ttcgcgcaac gaatacacca
    24901 aattttcggc ccgcgcgcgt actttggcgc aggcgattgg caagcggcta cctgcgccat
    24961 ggaaatcgcc aaaaactttg tggggaggga atattgtatg catcagacca taactgaaaa
    25021 gattattgct cgccatgcca ggcgtgaata cgtccggcct ggcgagttcg tcgaagtcag
    25081 tgttgatcag acctggtcgg acgatttggg ttcaccgatt accttgggac ttctcaagtc
    25141 gaatcgcgta ccgcgcgtat tcgacgcgga ccgcgtattt gtgacggcaa tggtcaacgc
    25201 gccggcacat agtatcgaga ctgcggcggt gatgaaggac atccgcggac tgacaagcga
    25261 cttcgatata acactctacg aggccggtca ggccgggatt cacaatgccc tcggcatcca
    25321 gctgggcagg acgcttcccg gcgaattgat cgtgggaggc aattcgcacg cgtgcatggc
    25381 tggcgccctg ggctgctttg ccaccggcat ggggagcacc gatatcgccg cgatcctggc
    25441 gacagggcgg acttggctcg aggtcccccg cacaatacgc tatcgctaca ccggcaaact
    25501 aaaaaaatgg gttaccggca aggacctgat cctgcacacc atcggcctga tcggcgtggc
    25561 cggcgccgac aactgcgcca tggagttctg cggcgagccg atcgaacagc tcagcgtcga
    25621 ggaacgtctt tcgatgacca atatggcgat cgaggccggc gcccagaacg cgatcatcgc
    25681 tccggacgtc aagaccttcg catacgtaga gaagattgca aaacgcccgt tcgagccgat
    25741 ctacggcgat cccggcgcgg acgtatttca agaaatcgag atcaacgttg atcgccttga
    25801 accggtcatc tccgtccctt tcctgcccag caagacgact tcgatcagcc aactcgggga
    25861 agtaaaactc gatcaggtct atatcggatc atgtacgaat ggctggatca ctgacttgcg
    25921 ttgcgccgcg agcattctca aaggccgaaa agtcgccgac gggctgagag tgtttgtgat
    25981 accgagcaca acccagattt acaagcaggc gatcgacgag gggcttgcgc aaattttcat
    26041 cgaggccgga tgcgccctgt cgatgccaac gtgcggacca tgcatcggcg cgcaaatggg
    26101 cgttctcgcc gatggagagc gctgcctttc cacgtccaat cggaattttc caggcagaca
    26161 ggggcacgtt agaagcgaaa cctacctctg caaccccgca gtagccgctg cgagcgccat
    26221 caagggcttc atctgcgggc ccgacgatct ttgaagacac tcaaccatac agggatacgc
    26281 aatgtggaaa caaggtaacg catggttcct cggcgacgac gtagatacgg atcagatcat
    26341 gccggcgcgc tatcttgccc tgaagacggc ggaagatctc gcagagcatg cgctggaggc
    26401 cgtcgatcct tcatggccga ccattatcaa acccggagat ttcttgatcg cgggcgccaa
    26461 ctttggatgc gggagctccc gtgagcacgc ccccctcgga ctaaaagggc ttggcgtcgg
    26521 ttgcatcatc gcaaaatcgt tcgcgcgaat cttctatcgg aattcgatca atatcggcct
    26581 accgcttttc gttctccgcg acgacattca gcaccggtcc caggcccacc gggcatgggt
    26641 cgatctatcc gaggggcaaa taaagttcga cgaggccggc ccggtgctgc agggggtggc
    26701 gccggcccag atcgtccagg acatcctgat gcatggagga ctgatggcgt acacggccgc
    26761 acggtccgga aaggttcaag aggaaagcaa agaggctggc ggaaacgcct cctgacaata
    26821 accgggcgaa cgaaaaacac agcacgccca cataaggaag gagacatcaa gtggatagta
    26881 caaaggcgac cccagaaatc cgacgggcga tgcgcatcac gacagtaagc gcgatcgtag
    26941 gcacggccgt ggagtggtat gactattatc tttacgccac ggccgcggca acaatcatca
    27001 atcacctgtt cttcccgtcg tatgaccatc tgaccggtac gcttctcgcg tatgcgagtt
    27061 tcgcgatcgg gttcatcgtg cggccggcgg gcagcgtgat cttcggccga cttggcgacc
    27121 gcatcggccg caaggcggtg ctctacatca cgctgttgct gatgggcggg tccacgacgc
    27181 tcatgggcct gttgccaacg tatgcgagtg cgggcctctg ggcaccggtg ctgctgtgcg
    27241 tgctgcgcgc gctgcaaggc cttggttctg gcgccgaata cgcgggcgcc gtgctgatgg
    27301 tggtggagta tgcgccggcg aaacggcgcg gcttctatgc ctccctgccc tatatcggcg
    27361 tcgcgctcgg cctgctcttg tcgctggcga gcttccacct ctcgtcggtg atatccaagg
    27421 atcatttcga ggattggggc tggcgcattc cctttctgct gagcgcagtg gtggttttgc
    27481 tagggttgat aatccgcagc cggttgaagg aaacccctgt tttcgagcag atccgccgcg
    27541 acggcgccgt gtcgcgcgcg ccgatcaccg aagttctgag aacctcgctg cgtccgatgc
    27601 tttgcgcgtg gggcgcgcgc ctcggggaca actcccttgc ctatatttat gaatcgttcg
    27661 tgattgtcta tgtcacgcag caattgaaat tgccgagaga cctcattgtc accgcgctga
    27721 tgtggggttc ggtcttgcag tgcgtcacga ttccactctt cggctggatg tcggatctca
    27781 tcggcaggaa gccggtctac atgctcgggg cgatcatatc cgggatagcc gtcttcccat
    27841 tcttttggat gttgcagaca aaagatcctc tcatgatcta cctggggctg ttcttggttt
    27901 cgcccgtcgc caagatcatg atgacgtccg cgcaaagcgc atggctagcg gagatgttcc
    27961 cgtctcgcgt gcgctatacg ggtttcgcgc tcgcccgcga agtgacatcg ccgatttccg
    28021 gcggcatcgc gccgatggtc gcggtggcgc tgctcgcggc cggtggcggc tctccccacc
    28081 tggttgcatg gtacgtggtg gcactgggtg ccatcaccac ggtatccgtg ctgctcggac
    28141 cagaaacacg cggccgctcg ctggatgaaa cgacacagga tctcccgcac gcacccattc
    28201 ggcaagccgc cgtttcccag aacgtacatt gaccttcgtc aagtacgcca gcccaggccc
    28261 aggcttttct gcagcgagac gtagtcctgc aggagcgagg cctggccggc cacgacgtcc
    28321 tgctgcgccg agtattcgac gcgctgggca tcgagcaggt cgatcaggct ggacgcgccc
    28381 gccgcgtagc gctggcgcgt caactgcgcc gcgcgcccgg cggagacctg cacctgctgc
    28441 aggcgcagca cgtgctcgcg ctgatgcccg tagcgcgaca aggcgtcatt ggcatcctgc
    28501 aaggcggaca gcacggtttg ctggtagcgg gcgtcggcct cgtcgcgctc ggcctcggcc
    28561 tggacgatgg cctggcgggt acggccgaag ctcaggaaat tccactgcag gaagggaatg
    28621 cccatccacg tgaaactgtc cttgctcagc acgtcgccaa agctcgtcga ggtaaagccc
    28681 agcacgccca tcagcgtcac cttgggaaac aggtcggccg tcttggcgcc gatctgcgca
    28741 tagctggcgg ccagcctgcg ctcggcggcc cggatgtcgg gacgctgctt gagcagcgcg
    28801 cccgggtcgc ccacggggac gctggccggc atgacgggca ggtcgcccgc cggggacaat
    28861 tgcgcgtcca ggtcgcccgg ggccttgccc gtcagcaccg ccaggcggtc cagcgactcg
    28921 acgatctggg cgtccagcgg cagcagggac gcgcgcgtcc cctcgacctg gctggtgagc
    28981 cgctccacgt cgaggtcggc cgccgtgccc gcggcccggc gccgccgggt caggtcgagc
    29041 attttctgct gcacctcggc cgattgctgc gccaggcgca ggcgctgctg ctcgtcgcgc
    29101 agcccgatat aggcctgccc aacctccgcg gccagcgaca cctgcacgtc ggccagatcg
    29161 gccgacacgg cctcggcctg cgcggcggcc gactcggtgg cgcggcgcac gccgccgaac
    29221 aggtcgagct cccaggaggc atcgaaactg gtggaataga gctgcagggg tccctgcccg
    29281 ccggattgct gcgactcgcc ggactgctgc gacgagccgc ccagcagcga cccgacgttc
    29341 ggcgagcgca ggcgcggcgc cgcgacatcg gccgtcaggc tgggcgtcaa ggccgcccgc
    29401 tgctgcccga gctgggcgcg ggcctggcgc aggcgggctt cggctgcgtg cacgtcgggg
    29461 ctctgcgcca aggccgcttc gatcagggcg gtgagcgtgg cgtcgttcaa ctgccgccac
    29521 cagtcgctgg gcgcagcctg cggcacgacg ccggccgtgg cgggcgcgcg cgcataggcg
    29581 ggcgcggaca gcgcggcttg ggcgacaggc ggggcatcgt ggtagtccgg gccgaccgcg
    29641 cagcccgcca acaccaccgc cgaacccgtc aacaccgtca acagcgcggc aacccgcggc
    29701 acaaggtgaa acgtcatgcg catcgtcgca ggcattcagt gctccatcgg cttgggcggg
    29761 ccgctcctgg gcgtcttgag cagcagcgcc agcggtatgc aggccaggag cgcgattgcc
    29821 agccagtaat aggtatcgat ataagtcatg accagggcct gctgctgtat ctgccgggcc
    29881 agcaggccca ggctgcgcag ctgcgcgtag gccaggtcgc cggtgcgggc gaaccagctg
    29941 gcggacgtgt tggccacata gtcctgcgcc aaggtggaat tggcggtgat cgattcacgc
    30001 aacgtggcca cgtggtaggt cgtgcgccgg tcgatcagga tgccgatgag ggccaggccc
    30061 accgagccgc cgaggttgcg cgccatattg aagaagcccg cggcatcgcc ggagtcctcg
    30121 cgcgaaacgg ccgccatcga ggcctggttg agcggcatca tggccaggat ctgcgccacg
    30181 ccgcgcagga actgcgaacc gtagaaatca tggcccacgc tctgcgccgt gatgcccagg
    30241 tccagccagc agctgaccgc gaacatgagc aggcccggga tcaccagcag gcggaagtcc
    30301 atcttgccca ggaggcgcgg caggatgggc atcatcagga acgacggaat gcccgagacc
    30361 agcatgatgc ccccggcctg ctcggcgttg tagccggcga ccagcccgag gaactgcggg
    30421 atcaggtagg acaccccgta caggcccgcc cccaccgcga acacgatgag gatcacgctg
    30481 gcgtagctcg gattgcgcat cagggacagg cgcatgaccg gccgctcggc cgtgaactgc
    30541 gacagggcga tcaaggccat gccgacgacc gacacgatgg tgagcgtgac gatcatggac
    30601 gaatcgaacc agtgctcgcg ctgcccttct tccagcacga cggtcagcga gctgagcccc
    30661 atcgccaggc cggcgatgcc cacccagtcg gccttgatga agtcgcccca gcgcgcgcgc
    30721 tcggtgggca ggccgatgat cagcagcacg gccagcatcc cgcagatggg catattgacc
    30781 aggaagcacc agtgccagct gatgttctcg gccagccagc cgcccaccac gggcccgagc
    30841 agggggccga gcagcaccgt caggccgaac accgtcatgc cgatgggcat ctgcgagcgc
    30901 ggcaggcgcg agcggatgat ggtctgcgcc gtcgggatca tggcgccgcc ggtaaagccc
    30961 tggccgatgc gcccggcgat catctgcccc aggctgcccg acatgccgca catgatcgag
    31021 aacagcgcga acagcaccgt attgcccagc aggaagttgc gcaggccgaa cacgcgggtg
    31081 agccaggccg ccagcgggat catcacgatt tccgacatca ggtagccggt ggagatccag
    31141 gtgccttcgg tgccggtggc gccgatcgcg ccctggatct gcggcaatgc cgagttggtg
    31201 atcgagatgt cgagcgtggc cgtcagcgcg cccagcgaac cggcggccac cgcgatccag
    31261 tcgccgaccg tggcgcgcgg ctcctcgtgg gcggcgccgg cgtcaaccat gattgccgtc
    31321 ctggcgcgtc tcgacgtcca cggtcaccga caggccgggc agcagccggt cggcgttccc
    31381 gcccgcctcg acgcggatgc gcaccggcac ccgctgcacg atcttggtga agttgcccgt
    31441 ggcgttttcc gggggcagca aggcgaactg cgagccggtg cccggggcga agctgtcgac
    31501 cacgccatgc agcttcacgc ccggcagcgc gtccaccgac agggtggcgg gctggccggg
    31561 acgcatgcgg cccagctgcg tttccttgaa attggccacc aggtagacgt cctgcaccgg
    31621 caccaccgtc agcatgcggg tgccgggctg cacgaactgc ccgacccgca cgctgcggtc
    31681 gcccacccgg ccggcgatcg tggcgcgcac cagggtgtcc tgcatgtcga gttcggactg
    31741 gcgcgcgctg gcctgcgcgg cggtcagctg cgcgcgcgac tgcgcgatct gcgtggtggc
    31801 cacctggatc tgggtcctgg ccacgttgac ggccgcattg ttggccgcca gcgccgccag
    31861 ggcctggtcg cgctcgctca ccaggtccgc caggcgttcg ttggtttccg ccccggccgc
    31921 ggccagcggc gtgtagcggc ggacctcttc gctggcatgg cgggccttga tgtcggccat
    31981 gtgcgcctgg gcctcggcct ggaccgcgtt ggcctgctgc tgctggattt ccgcctcgga
    32041 acgggcgatg tcggcctggc gcgcggcaat cgtcgcattg gcctggtcga aggaggcctg
    32101 gtattggcgc gcgtcgatgc gcaccagcgg atcccccgcc ttgaccgcct ggttgtcccc
    32161 gaccaggacc tcggccacat agccgctgac cttgggcgcc atggtgacgc tgtcggcctg
    32221 cagataggca tcgtccgtgc tttcgatgaa acgccccgcc agcagccagt acgccaacca
    32281 ggcaaccaca gccaggataa tgagcatgcc tattgcaata aaatatttcc gcttgccaga
    32341 acgggcgctt gccgcatcct ggggaactgc tgccgctgaa actggggggg aactggacat
    32401 aggcacgacc ggggcaatga tggatggcgt gattttatag gttggcgccc gccggtttca
    32461 cgatcagcac attccgtgcc ggaattcctg tccccgacct tacaatccgt accagttacg
    32521 aaggacaacc cgccttgtcg ccctgacgag tcaacggaga tactcgatgt ttcgagcagg
    32581 tcaactcaac ctccactaca gcgccttggt cgtcgcatcc ggccttgcct gcgcgctctg
    32641 caatggcctg ccgttcttca tcctgaccca gcacttgggc ctcggggaga tgccggacat
    32701 ccactgggcg cggggcctgg tcgtcatggc ggcctttttc tgcttctcga acttcaggct
    32761 gctcatcacg gcgcgccacc tgtggtggat actctaccgc ggcttcttcc gctggatctg
    32821 ggtgctgctc gccgccgtgc tgggcctttt ctatatggtg cacacccagg aagagaccga
    32881 gcgggcgcgg cagctgatct cccagtgggc gctggtggcg ctgcccctgc agctggcctg
    32941 cctggccgcc ctgcgcggct tcatccacca gatcaacaac ctggcgggga accggcgcaa
    33001 ggccgtgttc ttcggcatga gccctcaggc cagcaccctg tacctgcgct tgcagcgctc
    33061 tcccatcctg ggcatcgagg tcaccggcta ctacgccgaa gcgcctgtcg ccagcaactt
    33121 ccatgggctg gtgccgcctt acctgggccg ctacgaaacc gcctggccgc gcatacaggc
    33181 ctccgaattc gacatggtgt tcatccagcc ctacatccat tacggccagg agcgcagcga
    33241 ccagatcttc gagcagttgt acaactcgac atcgaccatc tacatggtgc cggaaaccca
    33301 gtggatatcg aacgtcacgc tctcgagcgt ggacattgcc gggatcacgc tcctgtcggt
    33361 ccacgacacg cccatcatcg gcgccgcccg ccagctcaag cgcctgatgg acctggtgat
    33421 cggcggcatc gcattcctgc tgctgtcgcc ggtcatgctg gcggcggccc tggccgtgcg
    33481 gctcgacacg ccggggccca tactcttcaa gcaagtccgc tacggcgaac gcggccagcc
    33541 cttccatgtc tacaaattcc gctcgatgta caccgacacg ggcgggccgc tgcggcaagt
    33601 cagccagagc gatccgcgcg tgacgcgcgt gggccggttc ctgcgccgca catctctcga
    33661 cgaactgccg caattgctca acgtgctgct gggctcgatg agcctggtcg gcccgcgccc
    33721 gcacgccgtc gtccacaacg agctctaccg ccagcagatc aacggctaca tgctgcgcca
    33781 tagcatcaag ccgggcatca ccggatgggc gcaggtcaac gggctgcgcg gcgagaccga
    33841 caccctggag aaaatggagc gccggctcga atacgaccgc tactacatca cgcactggtc
    33901 gctgggcctg gacttctgga ttctgctgcg cacggtctgg accgtggtgc acggcaacaa
    33961 cgcctattga tacggcaggc tcagcggtcg cgccactgcc gccacagcag gccgaagaac
    34021 ttcaggtcgt cgtacagata gcggcgaacc agccggcgcg gctcgctgcc catgcgccac
    34081 agccattcca ggccgttgcg ctgcatccag accggcgcgc ggctctgcag gccgatggca
    34141 aatcgcatgg ccatgcccgc gcacaggatg atgccgccgg gcagcgtcgg cccgtagcgg
    34201 aaggcccact tctcctgctt gggcatgccc aggcaggcga acacgacctg cggcgcaagc
    34261 tcgcggatgc ggcgcgcgtg ctcctccgct tcgggcccct cgggatcgta tcgcatggag
    34321 gcgctg
//
LOCUS       DASWPO010000349        22204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2675851, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000349 DASWPO010000000
VERSION     DASWPO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 22204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22204
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675851"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1288)
                     /gene="gabT"
                     /locus_tag="VGC69_18670"
     CDS             complement(26..1288)
                     /gene="gabT"
                     /locus_tag="VGC69_18670"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY0297358.1"
                     /translation="MQNLDTTTRRQLATPRGVGVQFDFYAARAENATLWDTAGKQYID
                     FAGGIAVLATGHRHPKVIAAVTEQLNQFTHTAYQIVPYESYIALAEKLAVLAPIDGLK
                     KAAFFTTGAEANENALKIARVHTGRPGVVAFTGAFHGRTHMTVTLTGKVAPYKLALGS
                     MVPDVFHVPFPNATQGTSVADSIKALDLLFKSSIDPKRVAAIIIEPVQGEGGFNITPP
                     ELMVALRKVCDEHGIVFIVDEVQTGFARTGKMFAMEHHSVQADIITMAKSLAGGFPLS
                     GLVGRAEIMDAPAPGGLGGTYAGNPLSVAAALAVLDVIQEENLCARAAALGDKLAARL
                     KQLQAGNKKIADVRNLGSMVACELVDANGEPDAAAVSNVIKQAAEKGLILLSCGVYGN
                     VLRFLYPLTIPEAQFDRALDILSELLAK"
     gene            1471..2928
                     /locus_tag="VGC69_18675"
     CDS             1471..2928
                     /locus_tag="VGC69_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0297359.1"
                     /translation="MNQRLYRCLREAILDGTIAADTRLPASRDLAAELGIARNTVMHV
                     YAQLQAEGYTRSRQGSGTYVTDTLPDAYLASGRNARRASMVPARPALSARGARIVDGA
                     LASPFQWGAFMPGVPDVTEFPHQKFGRIAGALWRKPPPELLTYSYGGGLPALRETLAQ
                     HLAITRSIDCDPEQILLTEGVHQGIDLIARILGEAGDTAWIENPGYWGARSVLQANGI
                     RAVPRAVDEEGMHLPTEPVAAPPRLLFVTPSHQYPLGPVMSLARRRQLLALARQWGSW
                     IVEDDYDSEFRFTGRPIPCLQGLEPDTPVIYLGTFSKTLYPGLRLAYLVLPKALVPAF
                     QAAHAELYHEGHLMTHAAVATFMAEGHYAARVRRMRMLYGRRRAMLVNLIERRLGQHW
                     LHRDSSDAGLHLVLDLPEDLSDEAVVRAAHARGVLTRPLSRYYDASDGKPGGEPPRQG
                     LLLGYACVPEADIARKFEVLLDSLAEVARLRGKAS"
     gene            3016..4011
                     /locus_tag="VGC69_18680"
     CDS             3016..4011
                     /locus_tag="VGC69_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEY0297360.1"
                     /translation="MSDESSPLPPEVMASVAYLEGRRECEVPIEDIPGYIGREDRLLW
                     IGLREPTEDLLVRVLAQLGADSRCREDMLEPHARPKVLDYGDLVLVVAIMVEVQAERP
                     MFGETQILIGEGFLLTVRRGSVAPYATLRARLESVPGLLKRGSDYVAAELLDLLVDRY
                     AVALHRLESQVEAAEQKLLIRGSGMADIRKLYRQRRDLLRIHTALAPMVEISRRLARV
                     EMGPIDAQARPYFGEVADRVQRIDELINALREALVFAFEASQMMAQAQQNDTTRKLAA
                     WAAILAVPTAVAGIYGMNFEFMPELHWHWGYYMVLLGMAAVCGTLYWRFRRVRWL"
     gene            4032..4685
                     /locus_tag="VGC69_18685"
     CDS             4032..4685
                     /locus_tag="VGC69_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY0297361.1"
                     /translation="MNSYERQAVRGLAGGILGRRLGAGLSVLVLAAAGVLLHRYLPST
                     GGTGAAHASSPGSSVPAGAYDLVGTIVKVADGDTVTLLTDERQQHRIRLDSIDSPEAS
                     HGADEPGQPFAEAARQNLAALVAGKHLTARCYETDQYRRDVCALMLDDGSSANRAQVA
                     GGYAWAYTARHGAYLRDGAMPALQRQARQAGLGLWARPGAGEPWKWRYDCWKQRQCQ"
     gene            4685..6958
                     /locus_tag="VGC69_18690"
     CDS             4685..6958
                     /locus_tag="VGC69_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297362.1"
                     /translation="MPRALAACLAWAWVLMLALAGLSGCSPDNPINSPYAGGSLASNT
                     FYTAFVGRSPKYLDPASSYSTDETPYTYNIYETLYGYHYLKRPYELVPRAAAAIDPPR
                     YLDAQGHVLPADAPGDQIAESVYDIRIRPGIRYQPHPALARRADGSYAYYPVPADVLA
                     HAFALTDFAQTGTRELTAQDFVYAFRRLASPRVVSPIYSLMADYVVGLKDYGDALRKR
                     DAALRQGLPPGTRDLPWLDLREPDGFDGVQALDGHTLRIRVKGKYPQFKYWLAMTFTA
                     PVPWEADRFYSQPGMAGHDLSLNTWPIGTGPYMLVESLPNRRHVLARNPNFHGEPYPC
                     EGEPGDREQGLLDDCGKPTPFIDRIVFSIEKESVPLSGKFLQGYYDVPQVERGDYGVA
                     MRVAAGDSADKARLYREHGIRLPSTVETANWYMGFNWLDPVVGRGDTPEQQEKNRKLR
                     QAISIAFDWEEHVAVFEDGQAQVAHGPVPPGVVGFQNLPAGYNPEVYDLVDGKPVRKS
                     LDVAKRLLAEAGYPSGRSSRTGEPLVLYYDAMSGAGGDPQFDWMRRQLAKIGIQMDVR
                     STDYNRFQDKMRRGAAQLFMWGWNADYPDAENFLFLLYGPNGKAQHGGENAANYENPE
                     FDKLFEQMKYLDDGPGKARLVARMVAIVRHDAPWMFGYFPLSGGAYQQWVGNAKPTQM
                     VRNTLQYMKLDTALRVKSVLAWNRPIWWPLPLLALLLCLAVRPAWAALRRRERQAALP
                     PVRPAVPPGSPGPGGRP"
     gene            6955..7932
                     /locus_tag="VGC69_18695"
     CDS             6955..7932
                     /locus_tag="VGC69_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297363.1"
                     /translation="MIAYIARRLLYGVLILLGVNLLTFVLFFAVNTPDDMARLAIGGQ
                     RTSEEAVAKWKVEHGYDKPLFYNAARHGAARYTDTIFYQRSVPLLAMDFGASDAGRDI
                     DREIGARMGPSLALAVPTFILGLAASVAFALLLVYFRATRLDFWGVVACVVMLSISGL
                     FYIIAGQWLFSKVLRLVPYSGYSGGLDVLKFLALPVVVAVVSRLGPEARFYRALFLEE
                     IGKDYVRTARAKGLTERVVLFRHVLKNAMLPILTGTVSAIPLLFMGSLIAESFFGIPG
                     LGSYTIDAINAQDFSIVRAMVFLGAALYIVGLILADISYTLADPRVRFK"
     gene            7951..9480
                     /locus_tag="VGC69_18700"
     CDS             7951..9480
                     /locus_tag="VGC69_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297364.1"
                     /translation="MFKPILLWTDAALFLLLACVLAYAWHAARSATLRAAWAQVSRSA
                     PAMCSAVVLLVMAAIGLLDSLHYQPRLPPVPGAPADAPAVYAPAVRSALDGLLDATVL
                     TRTEKTYSAPLAWRQFTKETLLVDGQPASAGPSQAGDPPRGDGAQRQGGAVIRDFPRL
                     RHGGAYLTDPGAQWLPDLRMRLGWGLAGGALAALLAGALLGAVLARRHGGWRAAVSAL
                     CRGRTDLPWRPVFCTVAALCLLVGAVAGLASGYHVLGTDRTGNDVLWQSLKSVRTALV
                     IGSLTTAAMLPPAIFFGIASGYFKGRVDDAIQYLYTTITAIPGVLLVAACALMMQVYI
                     DRHASLFNTSAARADLRLFLLCMILGLTGWAGLCRLLRAETLKLRELDYVQAARAFGI
                     SHWRIMRRHLLPNVMHLVLITVVLEFSGLVLYEAVLSYLGIGVDPSMNSFGAMIDGAR
                     LEMSRDPMIWWNLFAAFVFMLALVLAANLFADAVREAFDPRLRRRKAGKPVAADAGAL
                     P"
     gene            9477..11156
                     /locus_tag="VGC69_18705"
     CDS             9477..11156
                     /locus_tag="VGC69_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297365.1"
                     /translation="MNAPLLDVTDLSVDVDGEFGLVRAVKRLRFAVARGETFALVGES
                     GCGKSLTALALLRLLPEAGRIAGGQAWLEGGDLNQLPEAAMRTVRGGRIGVIFQEPAT
                     SLNPVMRVGDQIVETLRAHTDLRGAAARERAVHWLSRVGIPDPAQRLDDYPFQFSGGQ
                     KQRIMIAIALAAEPSLLIADEPTTALDVTVQAQVLALLADIQRELGMAVLLITHDLAV
                     VRQVAHHVALMRAGEIVESASAADFFAAPRHAYARELFDAIPTYAKRGRPLSAVGRAA
                     WAAGSGQPAARAPGEVVLDVRDLRVHYPVRHKAWRRDWVKAVDGVSFTLRAGETLALL
                     GESGCGKTTTAKALLRLAEGARVTGQALLADRDVLTASRGELRRLRRDIQIVFQDPYA
                     SLDPRMRVGEILDEGIAALRPELPAAQRAGRARSLLARVGLPANAAQRYPHEFSGGQR
                     QRIAIARALAVEPRVLVCDEPTSALDVSVQAQILDLLRELQAELGIAYLFITHNFSVV
                     EYLADRVAVMHGGRIVECDTAHAVLHAPSHAMTRRLLEAVPRLDFGAGPRA"
     gene            complement(11255..11860)
                     /locus_tag="VGC69_18710"
     CDS             complement(11255..11860)
                     /locus_tag="VGC69_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0297366.1"
                     /translation="MNPSHLTHPNPEKRMLEQPNDRVYFLQERRNDQREESEEVSARS
                     GAIADITLVMNRRLGVYLAANQAAIAEKLDLSVTELKALELVQELGALPTGQLAQLMG
                     ISWGGTTALINRLEAAGYVERGRHPLDRRIIVIRPIKEKCQELVQAQQALADEVSFIG
                     RQFDTQQLHAVQAFLTQYMRALRHDALAWLHARPGVETWND"
     gene            complement(11888..13921)
                     /locus_tag="VGC69_18715"
     CDS             complement(11888..13921)
                     /locus_tag="VGC69_18715"
                     /inference="COORDINATES: protein motif:HMM:NF027314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like triple helix repeat-containing
                     protein"
                     /protein_id="HEY0297367.1"
                     /translation="MATLAAALSACGGGGGGIGANDGAPLASLDTSPALTDGLTGGNG
                     TGTGGDGTTPCAVCGITGTPGGGDGGGSGGSGTGTGDSGTGGSGGNGSGTGTATAGVL
                     QTALGTTGSAIDNLIPLGIQTPLSNAGAGADGLVQPVVNATTGLTQQIGKTTGLGSPA
                     DNLLTQVGGAVSSLGTQVSGAAGSTGNLGGVATGVGGLLTGVGSGVAAAGGLLDPNSP
                     SASQPLTTAVGDIVGGVNALTASLGGDSGPLAPLGTALAPLVGGALDNPTLGVTKPAL
                     TNLGTAVDNVAPLGLTATLGGVGATVDPLLTPVSNIVTNVGDKVGDATGLGAPVSGLL
                     QQVGAGVTKLGAQTGGLAAPLAGVGAGIADLGGLVKLDPNNPSPLGSALTDVTGGVKS
                     LTAGLMTPNATSGGTLQPGLTNTGAAVDNVLPLGATTVLANVGNAVDPVVDPAVSTVT
                     GLTQKVGAATKLGAPVASLLTNVDGAVTKLGDNSLAQKTGLSATLADVGSAVGSLGGV
                     VNAPTATANPVGSTLTSATAGVQALTSNLTGGLLNPITGSAGGATGGLGTVTAPVTAL
                     LAGVTGGVAGAPTGAGSLSNGSLTKTVSNVITAVTTTLASAAPIASTGSAGSAAAPLT
                     NLVGGVTNTLSTVVNGVTSATGSATGSTGAAGGLGGLGGLLGGLVTGVTGGLK"
     gene            complement(14313..16040)
                     /locus_tag="VGC69_18720"
     CDS             complement(14313..16040)
                     /locus_tag="VGC69_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY0297368.1"
                     /translation="MKIARTRFLPGVSFSALASCLLLNAPAAHADAPLRGNPVESLPS
                     IGRPSQPSAPAPTVAPIPTPEQQAVQARLAQRIVPSHFDVSGVHALPFAEVSGMLTPL
                     AGKETTVGDLVQQVNKITEMYRSRGYPLSFALLQNQNFENGLVVVTVVEGYIDKVDVT
                     GDIGAARARLEAIAQPLYTEKPLTQATLERVLNLMRTVPGVTITPNLDLPRRADGATE
                     LSVAATRRPVQVLGGVSDLGMGLQPLASVGTNSLTPLGEQVKLTGAIPLNGQDARYYA
                     GEVHVPIGHDGLAVKVDGYHYEAQPHDAAVESLGFRRTVTNDRLGLGLSYPLLLNNRR
                     SLTGTLGMYGVNGKDRYDLQGTDRWLQQDTRVRAATAELRYIDVLPERTTDVTLSVSH
                     GMTGLGASKDITSNYGYSAAPDVDLDFTRYNLTAKQSFALPAQFGAVFGLLGQYSPNK
                     LPSSEQVSYGRWRYAMGYPQGDQSGDKGIGVSAELNRRFPTGWQYVPTVQPYVMVDYA
                     RTWYNDPNLQSVNERHLASASVGVRLTDDKYYLFDLNVARPIGDLPANSSSRRLQFNA
                     NYSIFYDAL"
     gene            16619..16798
                     /locus_tag="VGC69_18725"
     CDS             16619..16798
                     /locus_tag="VGC69_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0297369.1"
                     /translation="MLTQVKNFWNDESGATAIEYGLIAGLIAVVIIGALTVLGTSLTD
                     LFTRVSDAVGSAGKS"
     gene            16932..17447
                     /locus_tag="VGC69_18730"
     CDS             16932..17447
                     /locus_tag="VGC69_18730"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY0297370.1"
                     /translation="MLRDTLWWAAYIAFGLWVAYSDFRMRRVPNHLLVIAAAAQGLWL
                     LGFSLGGTLPSVGAQGWRDALPAFALGLCFAVCWKQRLMGAGDVKFLAVQGLLLGIKP
                     WFAMLLLGSVLSGAHALMQVLSKRRGVAPRQRGGPYAAYLALAAIASVAFMPSNSPLC
                     SWCSSVWSTRY"
     gene            17417..17854
                     /locus_tag="VGC69_18735"
     CDS             17417..17854
                     /locus_tag="VGC69_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEY0297371.1"
                     /translation="MFFCLVYAILTYGVIMAAQQSINFAAEEGARRVLQWASDRPTRA
                     NAALTLAASTSAWVSGLGGMAATQIAVCDQSGTLTSNNANAATLCNGLVSTSDASRVA
                     VVVRYPYQANPLVPLLPGISYLVPTMLGAQASVQLADAGGQGG"
     gene            17920..18441
                     /locus_tag="VGC69_18740"
     CDS             17920..18441
                     /locus_tag="VGC69_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY0297372.1"
                     /translation="MAALEFALSITVMLMVVVGIVGFGALFWAQQKLSKAAGEGAQAA
                     LYASLTGQAVTPEQLQPLLQTAACTAVQREANLLIPSGSTALASGSGCSVQTALCSAL
                     PNGWSPAAGDAPAMCASVNLQYSLSNWPLVSLMATLAGSPLFRGANWFPTQLAAQAVV
                     QVTPSAVQSASSS"
     gene            18458..19432
                     /gene="cpaB"
                     /locus_tag="VGC69_18745"
     CDS             18458..19432
                     /gene="cpaB"
                     /locus_tag="VGC69_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY0297373.1"
                     /translation="MSRVTAQIAAVLLIVIAVVLGVLAWRLSQRPPAPPAPVVIASTR
                     NEPSRPTVPVVTAAKPIASGSRIEAGALTVEQWPMRPAQGHATLEGLVGQYVRLDISQ
                     GQPITDALLVKGLSTHLKPGERAVTIPVDEVSGVQNRVQPGDTVDLFYVVERGSEVPG
                     SQTRLLQARVPVLAYGMQSVDGAPPTEDNGKPSASRNNAPPPAPRNAVLAVPVEKVNE
                     LLLAARSGRLMMALRSPQDTDVPDPTLFTPRQAVLIARPDLTQEQKDHLKDPINSAYA
                     GDDLPQLAGPATPKPDAQPKPKPIKPRGKGAGGSIEVLRGSETQTVHY"
     gene            19474..20709
                     /locus_tag="VGC69_18750"
     CDS             19474..20709
                     /locus_tag="VGC69_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEY0297374.1"
                     /translation="MSAAHHGIGRSLQLLGLGLSLALAAMPAWAQQRAARPARAVPAK
                     AAVVSEIPSAPQGLEVPINGQLPLPLTATPTRVAVADPAVADVKVLAPSGGRPGSIML
                     LGRKMGATEVRVWTGNSREPQVWKVQVVAGLTNTGGVVQVDVKVVELSNTVMKEVGLN
                     WNGRSGPWSAGAGTGTGSALIQAGGFVGDLGYSIGKFTADLNLLQTNGLARVLAEPSL
                     VALSGQSASFLAGGEIPIPESGGLGTQNVVFKPFGIGLTVSPTVISRDRIALKVAPEA
                     SELDYSNGINLVNGTTTTIIPALRTRKADTMIELGDGESYIISGLVSRETMANVNKVP
                     FLGDLPVIGTFFKNVQYSQSDKELVIVVTPHLVQPIARGVALPMPGGRQEQSDGSTTA
                     WGYYLLGPAGNEQMPGFSR"
     gene            20721..22043
                     /locus_tag="VGC69_18755"
     CDS             20721..22043
                     /locus_tag="VGC69_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HEY0297375.1"
                     /translation="MNTSAKEGLTFQAAQFLFCSEDNAVAAALGQVLGGLGLLVQEVP
                     ALDTVSRRLGELAPSVVFLDFTADPVDVDQLMRSSDLARVLARVSPNLPRVAVGYSSR
                     PVGAIAALRAGVSDFIDLSGKPEDAVDVVQHLLAFPGEASAGSGAQRSVLMLGARSGV
                     GVSTLAVHAASFTQMRLHRWHEQRTGQPPLKQGKGEQEMLPLTERVSLLDIGYPVGDC
                     LLYLNLTSDFDFAEAARNINRLDGTMLSAAMAHTASGINVMSLPRDLTQLRDLSFADS
                     LSLYERLRQYSGIMLIDAGGHENIEFISQLGRACSDIWLVTDQSVSALVALDSLVAEL
                     GKQGIACERMRLVVNRYDERYGMDAGQIAQRFKLPLAGTLPDRTLSLMTCMNQGRLLH
                     EVTDRDPYVKSVRYLVGNLMDERFVQPARPQGKLSAWMHGSRRRSASF"
     gene            22053..>22204
                     /locus_tag="VGC69_18760"
     CDS             22053..>22204
                     /locus_tag="VGC69_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY0297376.1"
                     /translation="MIAPSMEFGSGNNSNEFASSQRFLDIKNQAYEHLLSRIEELGAE
                     FGRWTR"
BASE COUNT         3341 a         7314 c         7725 g         3824 t
ORIGIN      
        1 accccgcccg ccagtgttat gggaattact tggccagcag ttccgacaga atatccaggg
       61 cgcggtcgaa ttgcgcctcg gggatggtca ggggatacag gaagcgcagc acgttgccgt
      121 acacgccgca agacagcagg ataagacctt tttcggcggc ctgcttgatg acgttcgaca
      181 cggcggcggc atcgggctcg ccgttcgcgt cgaccagttc gcaggcgacc atcgagccca
      241 ggttgcgcac gtcggcgatc ttcttgttgc cggcctgcag ttgcttgagg cgggcggcca
      301 gcttgtcgcc gagcgcggcg gcgcgggcgc acaggttttc ttcctggatg acgtccagca
      361 cggcgagcgc ggcggccacc gacagcgggt tgccggcata ggtgccgccc aggccgccgg
      421 gggcgggggc gtccatgatc tcggcgcggc ccaccaggcc cgacaggggg aagccgccgg
      481 ccaggctctt ggccatggtg atgatgtcgg cctggacgct gtggtgctcc atggcgaaca
      541 tcttgccggt gcgggcgaag ccggtctgca cttcgtcgac gatgaacacg atgccgtgct
      601 cgtcgcagac cttgcgcagg gcgaccatca gctcgggcgg ggtgatgttg aaaccgcctt
      661 cgccctgcac gggctcgatg atgatggcgg cgacgcgctt gggatcgatg gacgacttga
      721 acagcaggtc gagcgccttg atcgagtcgg ccaccgaggt gccctgggtg gcgttgggga
      781 agggcacatg gaagacgtcg ggcaccatgg agccgagcgc cagcttgtag ggggcgacct
      841 tgccggtcag cgtgaccgtc atgtgggtgc ggccgtggaa ggcgcccgtg aaggcgacga
      901 cgcccgggcg gccggtgtgg acgcgggcga tcttgagcgc gttttcgttc gcttcggcgc
      961 cggtggtgaa gaaggcggcc ttcttcaggc cgtcgatggg cgccaggacg gccagctttt
     1021 cggccagggc gatgtagctt tcgtagggaa cgatctgata ggcggtgtgg gtgaactggt
     1081 tgagctgctc ggtgacggcg gcgatcacct tggggtggcg gtggccggtg gccagcacgg
     1141 cgatgccgcc ggcgaagtcg atgtattgct tgccggcggt gtcccacagc gtggcgtttt
     1201 cggcgcgggc ggcatagaaa tcgaattgga cgccgacgcc gcggggggtg gccagctggc
     1261 ggcgggtggt cgtgtctagg ttttgcatgg ggacctcgga tcggtgggac gccgtggcgc
     1321 ggcggaaaaa gaggggagag cctgtagcga aatttaatgc taggatggct ccattagtga
     1381 gagccacttc tgcaagattg gtggagccaa ttgcatacca ttgccggtga cctgctgctg
     1441 ctgcggctgg gcgaggcggc cgacggcccc atgaaccagc ggctgtaccg ctgcctgcgc
     1501 gaggccattc tggacggcac cattgccgcc gacacgcggc tgcctgcctc gcgggacctg
     1561 gcggccgaac tgggcatcgc ccgcaatacc gtgatgcacg tctatgccca attgcaggcc
     1621 gagggctata cgcgcagccg gcagggcagc ggcacctatg tcaccgacac cctgcccgac
     1681 gcctacctgg ccagcggccg caacgcgcgg cgcgccagca tggtgcccgc aaggccggcc
     1741 ttgtccgcgc gcggcgcgcg catcgtcgac ggcgcgttgg cctcgccttt ccagtggggg
     1801 gccttcatgc cgggggtgcc cgacgtgacc gagttccccc accagaaatt cggccgcatc
     1861 gccggcgccc tgtggcgcaa gccgccgccc gagctgctga cctactccta cggcggcggc
     1921 ctgccggcgc tgcgcgagac gctggcgcag cacctcgcga tcacgcgttc catcgattgc
     1981 gaccccgagc agatactgct gaccgaaggg gtgcaccagg gcatagatct gatcgcccgc
     2041 attctcggcg aggcgggcga cacggcctgg atcgagaacc cgggctactg gggcgcgcgc
     2101 tccgtcctgc aggccaacgg catccgggcc gtgccgcgcg cggtggacga ggaaggcatg
     2161 cacctgccga ccgaaccggt ggcggcgccg ccgcgcctgc tcttcgtgac gccatcgcac
     2221 cagtatcccc tggggccggt gatgtcgctg gcccggcggc gccaattgct ggccctggcg
     2281 cggcaatggg gcagctggat cgtcgaggac gactacgaca gcgagttccg cttcacgggc
     2341 cgccccattc cctgcctgca gggcctggag cccgataccc cggtgatcta cctgggcacg
     2401 ttcagcaaga ccctctaccc cggcctacgg ctggcctacc tggtgctgcc caaggcgctg
     2461 gtgccggcct tccaggccgc gcacgccgag ctgtaccacg aaggccacct gatgacgcac
     2521 gcggccgtgg cgaccttcat ggccgaaggg cattacgcgg cccgtgtgcg gcgcatgcgc
     2581 atgctgtacg ggcggcgccg cgccatgctg gtcaacctga tcgagcggcg cctggggcag
     2641 cactggctgc atcgcgactc cagcgatgcc ggcctgcacc tggtgctgga cctgcccgaa
     2701 gacctgtccg acgaggcggt ggtgcgtgcc gcccatgccc gcggcgtgct gacgcgaccg
     2761 ctgtcgcgct attacgatgc ctcggatggc aaacccggcg gcgagccgcc gcggcagggc
     2821 ctgctgctgg gctacgcctg cgtgcccgag gccgacatcg cccgcaagtt cgaagtgttg
     2881 ctcgacagcc tggccgaggt ggcccggttg cgcgggaaag catcgtgagg caaagccggc
     2941 ggccttgcgt aaactacgcc agtttcctcg ccgagccggt ccgcaccggc ctggcgcctt
     3001 gtggagaatg caggcatgtc tgatgaatcg agtcccttgc cgcccgaggt gatggcgtcc
     3061 gtggcctatc tggaaggccg gcgggagtgc gaggttccca tcgaggatat ccccggctat
     3121 atcggccgcg aggatcgcct gctctggata ggcctgcgcg aacccaccga ggacctgctg
     3181 gtgcgcgtgc tggcccagct gggcgccgat tcgcgctgcc gcgaggacat gctcgagccg
     3241 catgcgcggc ccaaggtgct cgactacggc gacctggtgc tggtggtggc catcatggtc
     3301 gaggtgcagg ccgaacggcc catgttcggc gaaacccaga tcctgatcgg cgaaggcttc
     3361 ctgctgaccg tgcggcgcgg ttcggtggcg ccctatgcca ccttgcgcgc gcgcctggag
     3421 tccgtgcccg gcctgctcaa gcgcggcagc gactatgtgg ctgccgagct gctcgatctg
     3481 ctggtcgacc gctatgcggt ggcgttgcac cggcttgagt cccaggtcga ggcggccgag
     3541 cagaagctgc tcatccgcgg ctccggcatg gccgacatcc gcaagctcta tcgccagcgc
     3601 cgcgacctgc tgcgcatcca taccgcgctg gcgcccatgg tcgagatcag ccgccgcctg
     3661 gcgcgcgtcg agatggggcc catcgatgcg caggcccgcc cgtatttcgg cgaggtggcc
     3721 gaccgcgtgc agcgtatcga cgaactgatc aacgccctgc gcgaagccct ggtgttcgcc
     3781 ttcgaagcca gccagatgat ggcgcaggcc cagcagaacg acaccacccg caagctggcg
     3841 gcctgggcgg ccatcctggc cgtgccgacc gccgtggcgg gcatctacgg catgaacttc
     3901 gagttcatgc ccgagctgca ctggcactgg ggctactaca tggtattgct cggcatggcg
     3961 gcggtctgcg gcacgctcta ctggcgcttt cgccgggtta ggtggctgta atcctttttc
     4021 gaggtgacgg gttgaacagc tacgaaagac aagccgtgcg cggcctggcc ggcggcatcc
     4081 tggggcgccg cctgggcgcc ggcctgagcg tcctggtgct ggcggcagcg ggagtcctgc
     4141 tgcatcgcta cctgccctcg acgggcggga cgggggcggc gcatgcttct tcgccgggca
     4201 gctcggtgcc cgccggggcc tacgatctgg tgggcacgat agtcaaggtg gccgacggcg
     4261 acaccgtcac gctattgacc gacgagcgcc agcagcaccg catccggctc gacagcatcg
     4321 actcgccgga agcgtcccac ggcgccgacg agcccggcca gccctttgcc gaggcggccc
     4381 gccagaacct ggccgcgctg gtggcgggca agcatctcac ggcgcgctgc tacgagaccg
     4441 accaataccg ccgcgatgtc tgcgccctga tgctggatga cggctcgtcg gccaaccgcg
     4501 cccaggtcgc gggcggctat gcctgggcct acacggcgcg gcacggcgcc tacctgcgcg
     4561 acggcgccat gcccgcgctg cagcgccagg ccaggcaggc cggcctcggc ctgtgggcgc
     4621 gaccgggggc gggcgagccc tggaaatggc gctacgactg ctggaagcag cgccagtgcc
     4681 agtgatgccg cgcgcgctgg cggcctgcct ggcctgggcc tgggtgctga tgctggcact
     4741 tgccggattg tcgggctgtt cgcccgacaa tcccatcaac agcccctatg ccggcggcag
     4801 cctggccagc aacacgttct atacggcctt cgtcggccgt tcgcccaagt acctcgatcc
     4861 ggccagttcc tactcgaccg acgagacgcc ctacacctac aacatctacg aaacgctcta
     4921 cggctatcac tacctgaagc ggccctatga actggtgccg cgcgccgcgg ccgccatcga
     4981 tccgccgcgc tacctggatg cccagggcca tgtcctgccc gccgacgcgc cgggcgacca
     5041 gatcgccgag agcgtctacg acatccgcat ccggcccggc atccgctatc agccgcatcc
     5101 ggcgctggcc cgccgcgccg acggcagcta cgcctattac cccgtgccgg ccgatgtgct
     5161 cgcgcatgcg tttgccctga ccgacttcgc gcagacgggc acgcgcgagc tgacggccca
     5221 ggattttgtc tatgccttcc ggcggctggc cagcccgcgc gtggtctcgc ccatctactc
     5281 cctgatggcg gactatgtgg tgggactgaa ggactatggc gacgcgctgc gcaagcgcga
     5341 tgccgccctg cggcagggac tgcccccggg cacgcgcgac ctgccctggc tggacctgcg
     5401 cgaacccgat ggcttcgacg gcgtgcaggc gctcgacggc cacacgctgc gcatccgcgt
     5461 gaagggcaag tatccgcagt tcaagtactg gctggccatg accttcaccg cgccggtgcc
     5521 ctgggaggcc gaccgcttct acagccagcc cggcatggcc gggcacgacc tgtcgctcaa
     5581 tacctggccc atcggcaccg ggccctatat gctggtggag tcgctgccca atcgacgcca
     5641 cgtgctggcg cgcaatccca atttccacgg cgagccttat ccctgcgagg gagagccggg
     5701 cgaccgcgag cagggcctgc tcgacgattg cggcaagccc acgcccttca tcgaccgcat
     5761 cgtattcagc atcgaaaagg aatcggtgcc cctgtcgggg aaattcctcc agggctatta
     5821 cgacgtgccc caggtcgagc gcggcgacta cggcgtggcc atgcgcgtgg ctgcgggcga
     5881 ctcggccgac aaggcccggc tgtatcgcga gcacggcata cgcctgccgt ccacggtcga
     5941 gacggccaac tggtatatgg gcttcaactg gctcgatccg gtggtggggc gcggcgacac
     6001 gcccgaacag caggaaaaga accgcaagct gcggcaggcc atcagcatcg ccttcgactg
     6061 ggaagagcac gtggccgtgt tcgaggatgg gcaggcccag gtggcgcatg gtcccgtgcc
     6121 gcccggggtg gtgggtttcc agaatctgcc ggccggctac aaccccgagg tctacgacct
     6181 ggtcgacggc aagcccgtgc gcaagtccct ggacgtggcc aagcgcctgc tggccgaggc
     6241 cggctacccc agcggccgca gcagcaggac gggcgagccc ctggtgcttt actacgatgc
     6301 catgagcggc gcgggcggcg atccgcagtt cgactggatg cgccgccagc tggccaagat
     6361 cggcatccag atggacgtgc gctccaccga ctacaaccgc ttccaggaca agatgcggcg
     6421 cggcgcggcg cagttgttca tgtggggctg gaatgccgac tacccggatg cggaaaattt
     6481 tctgttcctg ctgtatggcc ccaacggcaa ggcccagcat ggcggtgaaa acgccgccaa
     6541 ctacgaaaac ccggaattcg acaaactctt cgagcagatg aaatacctcg acgacggtcc
     6601 gggaaaagcc cggctggtgg cccgcatggt ggccatcgtg cggcatgacg cgccctggat
     6661 gttcggctac ttcccgctgt cgggcggggc ctaccagcag tgggtgggca atgccaagcc
     6721 tacgcagatg gtgcgcaata ccctgcagta catgaagctc gacacggcct tgcgggtcaa
     6781 gagcgtgctt gcgtggaacc ggcccatctg gtggcccctg ccgttgctgg ccttgctgct
     6841 gtgcctggcc gtgcggccgg cctgggcggc gctgcgccgc cgcgagcggc aggccgcctt
     6901 gccgccggtc cgtccggccg tcccgcctgg atcgcctggc ccgggaggac ggccatgatc
     6961 gcttatatcg cgcggcgcct gctgtatggc gtgttgatcc tgctgggcgt caacctgctg
     7021 accttcgtgc ttttcttcgc ggtgaacacg cccgacgaca tggcgcgcct ggccatcggc
     7081 gggcagcgca ccagcgaaga ggccgtggcc aaatggaagg tcgagcacgg ctacgacaag
     7141 ccgctcttct acaacgcggc caggcatggc gccgcgcgct ataccgacac gattttctac
     7201 cagcgctccg tgccgctgct ggccatggat ttcggtgctt cggacgcggg acgcgacatc
     7261 gaccgcgaga tcggcgcgcg catgggcccc agcctggcgc tggccgtgcc caccttcatc
     7321 ctgggcctcg cggccagcgt ggcgttcgcc ttgctgctgg tgtattttcg cgcgacgcgg
     7381 ctggacttct ggggcgtggt cgcctgcgtg gtgatgctgt ccatctcggg gctgttctac
     7441 atcatcgcgg ggcagtggct gttttccaag gtgttgcggc tggtgccgta ttcgggctat
     7501 tcgggcgggc tggatgtctt gaagttcctg gccttgccgg tggtggtggc cgtggtctcg
     7561 cgcctgggcc cggaagcgcg cttctaccgc gccctgttcc tggaagagat cggcaaggac
     7621 tacgtgcgca cggcgcgcgc caaggggctg accgaacgcg tcgtgctgtt tcgccatgtg
     7681 ctgaaaaacg ccatgctgcc catcctgacc gggacggtct cggccattcc gctgcttttc
     7741 atgggcagcc tgatcgccga gtcgtttttc ggcataccgg gcctgggcag ctacaccatc
     7801 gacgccatca acgcgcagga tttttccatc gtgcgggcca tggtgttcct gggggcggcg
     7861 ctgtatatcg tgggcctgat cctggccgac atttcctaca cgctggccga tccccgcgtg
     7921 cgtttcaagt aggcgacccg cggagccgac atgttcaaac cgatactgct ctggaccgac
     7981 gctgccctgt tcctgctgct ggcctgcgtg ctggcctatg cctggcatgc ggcgcgcagc
     8041 gccaccttgc gcgccgcctg ggcgcaggtg tcgcgcagcg cgccggccat gtgttcggcg
     8101 gtggtcctgc tggtgatggc ggcgatcggc ctgctggact cgctgcacta ccagccgcgc
     8161 ctgccgccgg tgcccggcgc gccggccgac gccccggcgg tgtacgcgcc cgccgtgcgc
     8221 tcggcgctcg acggcctgct cgacgccacg gtgctcacgc gcaccgagaa gacctattcc
     8281 gcgccgctgg cctggcgcca gttcaccaag gaaaccctgc tggtcgatgg gcagccggcg
     8341 agcgccgggc cgtcccaggc gggcgatcct ccccgggggg acggcgcgca gcgccaaggg
     8401 ggcgctgtca ttcgcgattt tccgcgcttg cggcatggcg gcgcctattt gacggaccct
     8461 ggcgcgcagt ggctgccgga cctgcgcatg cgcctggggt gggggctggc tggcggcgcc
     8521 ctggcggcct tgctcgccgg cgcgctgctg ggcgcggtcc tggccaggcg gcatggcggt
     8581 tggcgggccg cggtgagcgc gctatgccgg ggacgcacgg acttgccctg gcggcccgtg
     8641 ttctgcacgg tcgcggcgct gtgcctgctg gtcggcgcgg tggccggcct ggccagcggc
     8701 tatcacgtgc tgggcaccga ccgcacgggc aacgacgtgc tgtggcagtc gctcaagagc
     8761 gtgcgcacag cgctggtgat aggcagcctc accacggccg ccatgctgcc gccggccatt
     8821 ttcttcggca tcgcatcggg ctacttcaag ggccgcgtcg acgacgccat ccaatacctc
     8881 tacaccacca tcaccgccat tcccggcgtg ctgctggtgg ccgcctgcgc cctgatgatg
     8941 caggtgtata tcgaccgcca cgccagtctt ttcaatacct cggcggcgcg ggccgacctg
     9001 cgtcttttcc tgctgtgcat gatcctgggc ctgacgggct gggccggcct gtgccgcctg
     9061 ctgcgcgccg agaccctcaa gctgcgcgag ctcgactatg tgcaggcggc gcgcgccttc
     9121 gggatctcgc actggcgcat catgcgccgg cacctgctgc ccaatgtcat gcacctggtg
     9181 ctgatcaccg tggtgctcga gttttctggc ctggtgctgt acgaggccgt gctgtcctac
     9241 ctgggcatag gcgtggatcc gagcatgaat tccttcggcg ccatgatcga cggggcgcgt
     9301 ctcgaaatgt cgcgcgaccc gatgatctgg tggaacctct tcgccgcctt cgtcttcatg
     9361 ctggccctgg tgctggcggc caacctgttc gccgacgcgg tgcgcgaggc cttcgatccg
     9421 cgcctgcgcc ggcgcaaggc cggcaagcct gtcgccgcgg acgcgggagc cttgccatga
     9481 acgcgccctt gctcgacgtg acggacctga gcgtggacgt ggacggcgag ttcggcctgg
     9541 tgcgggccgt caagcgcctg cgattcgccg tggcgcgcgg cgaaaccttc gccctggtgg
     9601 gcgagtccgg ctgcggcaag agcctgacgg cgctggcctt gctgcggctc ctgccggaag
     9661 ccggccgcat cgcgggcggg caggcctggc tggagggcgg cgatctcaac cagctgccgg
     9721 aggccgccat gcggaccgtg cgcggcgggc gcatcggcgt catcttccag gaaccggcca
     9781 ccagcctgaa tccggtcatg cgggtgggcg accagatcgt cgagacgctg cgggcgcata
     9841 ccgatttgcg cggcgcggcg gcgcgcgagc gggccgtgca ctggctgagc cgcgtgggca
     9901 ttcccgaccc ggcgcagcgc ctggacgact atcccttcca gttctcgggc gggcagaagc
     9961 agcgcatcat gatcgccatc gcgctggccg ccgagcccag cctgctgatc gccgacgagc
    10021 cgaccacggc gctggacgtc accgtgcaag cccaggtgct ggcgctcttg gccgacatcc
    10081 agcgcgaact gggcatggcg gtgttgctga tcacccacga cctggccgtg gtgcgccagg
    10141 tggcccacca cgtggccctg atgcgcgccg gcgagatcgt cgaaagcgcg tccgcggccg
    10201 attttttcgc ggcgccgcgc catgcttacg cgcgtgaact gttcgacgcc attcccactt
    10261 acgccaagcg cgggcggccc ctctcggccg tgggccgggc ggcatgggcg gccgggagcg
    10321 ggcagccggc cgcgcgtgcg ccgggcgagg tggtgctcga tgtgcgggac ctgcgggtgc
    10381 attatcccgt ccggcacaag gcatggcggc gcgactgggt caaggcggtc gacggcgtga
    10441 gcttcaccct gcgggcgggc gagaccctgg cgctgttggg cgaatcgggc tgcggcaaga
    10501 ccaccacggc caaggccttg ttgagactgg ccgagggcgc ccgcgtcacg ggccaggccc
    10561 tgctggcgga tcgcgatgtg ctgaccgcgt cgcggggcga actgcgccgt ttgcggcgcg
    10621 acatccagat cgtgttccag gatccgtacg cgtcgctcga cccgcgcatg cgggtgggcg
    10681 agatcctgga cgaaggcatt gccgccctgc gtcccgaact gccggcggcc caacgcgccg
    10741 ggcgggcgcg cagcctgctg gcgcgggtcg gcctgcccgc gaacgcggcg cagcgctatc
    10801 cgcacgaatt ctccggcggc cagcgccagc gcatcgccat tgcgcgggcc ctggcggtcg
    10861 agccccgggt gctggtctgc gacgagccca cctcggccct ggatgtgtcg gtgcaggcgc
    10921 agatactcga cctgctgcgc gagctgcagg ccgaactggg cattgcctat ctcttcatca
    10981 cgcacaactt cagcgtggtc gaatacctgg ccgaccgggt ggcggtcatg cacggcgggc
    11041 gcatcgtcga atgcgacacg gcccacgccg tgctgcacgc gccgagccac gccatgacgc
    11101 gccgcctgct cgaggccgtg ccgcgcctgg acttcggcgc cggcccgcgc gcataaaaaa
    11161 cgcgccttgg gacaaggcgc gtatcaaaaa cggggaagcg ttcttggccg cttccgcgcg
    11221 aggggatccg accggtcagg acgggaaccg caactcagtc attccaggtt tccaccccgg
    11281 gacgggcatg cagccaggcc agcgcgtcgt gacgcagggc gcgcatgtac tgggtcagga
    11341 aggcctgcac ggcatgcagc tgctgcgtgt cgaactggcg gccgatgaag gagacttcgt
    11401 cggccagcgc ctgctgggcc tgcaccagct cctggcactt ttccttgatc gggcggatga
    11461 cgatgatgcg gcgatccagg ggatggcggc cgcgctcgac ataaccggcc gcttccaggc
    11521 ggttgatcag cgcggtggtg ccgccccagc tgatgcccat cagctgggcc agttggccgg
    11581 tgggcagggc gccgagctcc tgcaccagtt ccagggcctt gagctcggtc accgacaggt
    11641 ccagcttttc ggcgatggcg gcctggttgg cggccaggta gacgccgagc cggcggttca
    11701 tcaccagggt gatgtcggcg atggcgccgg agcgggccga gacttcctcg gattcttcgc
    11761 gctgatcgtt gcggcgttcc tgcaggaagt acacgcgatc attgggctgt tccaacatgc
    11821 gtttctccgg gttggggtgc gtgagatggg aaggattcat gggaggctgt tttccgcatc
    11881 cgccggctta cttcaggccg ccggtgacgc cggtcaccag gccgccgagc aggccgccca
    11941 gaccacccag gccgccagcg gcgcccgtgg agccggtagc cgaaccggtg gcggaggtca
    12001 cgccattgac taccgtgctc agcgtgttgg tcacgccgcc gaccaggttg gtcaggggcg
    12061 cggcggccga tccggccgaa cccgtcgagg cgatgggcgc cgcgctggcg agcgtggtgg
    12121 tcacggcggt gatcacgttg ctcaccgttt tggtcagcga gccgttgctc aggctgccag
    12181 ccccggtggg cgcgccggcg acaccgcccg tcacgccggc aagcagcgca gtcaccggag
    12241 ccgttaccgt gcccaggccg ccagtcgcgc cgccggccga acccgtgatc gggttgagca
    12301 ggccgccggt caggttggag gtcagggcct ggacgccggc ggtcgccgag gtcagtgtcg
    12361 aaccgaccgg gttggccgtc gcggtggggg cgttgaccac gccgcccagg ctgcccacgg
    12421 cgctgcccac gtcggccagc gtggccgaca ggccggtttt ctgggccagc gagttgtcgc
    12481 ccagtttggt cacggcgccg tccacattgg tcagcaggct ggcgacgggc gcgcccagct
    12541 tggtcgcggc gccgactttc tgcgtcaggc cggtcaccgt ggagacggcg ggatcgacga
    12601 ccgggtcgac ggcgttgccg acattggcca gcacggtggt cgcgccgagc ggcagcacat
    12661 tgtcgacggc cgcgccggtg ttggtcaggc cgggctgcag cgtgccgccg gaggtggcgt
    12721 tgggcgtcat caggccggcg gtcagcgact tcacgccgcc ggtgacgtca gtcaaggccg
    12781 aacccagcgg gctgggattg ttcgggtcga gtttcaccag gccgccgagg tcggcgatgc
    12841 ccgcgccgac gccagccagc ggcgcggcca ggccgccggt ctgggcgccc agcttggtca
    12901 cgcccgcgcc cacttgctgc agcaggccgg aaaccggagc gcccaggccc gtggcgtcgc
    12961 cgaccttgtc gccgacattg gtcacgatat tggagaccgg agtgagcagg gggtcgaccg
    13021 tggcgccgac gccgcccagg gtggcggtca ggccgagcgg cgcgacgttg tcgaccgcgg
    13081 tgccgagatt ggtcaatgcg ggtttggtca cgcccagggt cgggttgtcg agcgcgccgc
    13141 ccacgagggg ggccagcgcc gtgccgagcg gggcgagcgg gccgctgtcg ccgccgaggc
    13201 tggccgtcag cgcattcacg ccgccgacta tgtcgcccac ggccgtcgtc aacggttgcg
    13261 aggcgctggg cgagttgggg tcgagcaggc cgccggcggc ggccacgccg ctgcccacgc
    13321 cggtcagcag gccgcccacg ccggtggcca cgccgcccag attgcccgtg ctgccggccg
    13381 cgccgctcac ttgagtgccc aggctggaca cggcgccgcc cacttgggtc agcagattgt
    13441 cggcgggcga gcccaggccg gttgtcttgc cgatttgctg ggtcaggccg gtggtggcgt
    13501 tgaccaccgg ttgcaccagg ccatcggcgc cggcgccggc attgctcagc ggcgtctgga
    13561 tgcccagcgg aatcagattg tcgatggcgc tgccggtcgt gcccagcgcg gtttgcagga
    13621 cgccagccgt ggcagtcccg gtgcccgagc catttccgcc cgaaccgccg gtacccgagt
    13681 cgcccgtgcc cgtgcctgaa ccgccgctgc cgccgccatc gccgccgccg ggcgtgccgg
    13741 tgatgccgca gactgcgcag ggggtggtgc catcgccgcc ggtgccggtg ccattaccgc
    13801 cggtcagtcc atcggtcagc gcgggcgagg tgtccaggct ggccagcgga gcgccgtcat
    13861 tggcgccgat gccgccgcca ccgccgccgc aagcggaaag cgcagccgcc agggtagcca
    13921 tgcaaaaggt tgccgatgca cggcggaagg ggctgcgaga agccgcttgc gtattgatct
    13981 tgacagacat ggcactactc ctgaagtgga ctggtacaag gccgttctcc ggctgcttgt
    14041 cccttcagtc gcataaaccg tgccaacttt catcgagcta tcggtcaagc gtctcaagaa
    14101 cctttttaat gcgggtgtgt atttattaaa ttcctttttt atcagtaggt tatgaggtgt
    14161 attttcagta atttaaaatg caaataaatt gcaaatttac gatttcctaa cgtttgagat
    14221 agtgattttc gtatgaattt cgttccgatg ttacggtcgg cgttacgtta cgaacggccc
    14281 ggaaccgggt gggtaacggg ccacacgcgg acttacaggg cgtcgtagaa gatcgagtag
    14341 ttcgcgttga actgcaggcg gcggctcgag ctgttggcgg gcaggtcgcc gatggggcgg
    14401 gccacgttca ggtcgaacag gtagtacttg tcgtcggtca ggcgcacgcc caccgatgcc
    14461 gatgccaggt gccgctcatt gaccgattgc aggttggggt cgttgtacca ggtgcgggcg
    14521 tagtcgacca tgacgtaggg ctgcacggtc ggcacgtatt gccagcccgt ggggaagcgc
    14581 cggttcagct cggccgacac gccgatgccc ttgtcgccgc tctggtcgcc ctgcgggtag
    14641 cccatggcat agcgccagcg gccgtacgac acctgttccg aggacggcag cttgttgggg
    14701 ctgtactggc ccagcaggcc gaacacggcg ccgaactggg ccggcagggc aaacgactgt
    14761 ttggcggtca ggttgtagcg ggtgaagtcc aggtccacgt ccggcgccgc gctgtagcca
    14821 tagttggagg tgatgtcctt gctggcgccc agtcccgtca tgccgtgcga cacgctcaag
    14881 gtgacatcgg tggtgcgctc gggcagcacg tctatatagc gcagttcggc cgtggcggcc
    14941 cggacccggg tgtcctgctg cagccagcgg tcggtgccct gcaggtcgta gcggtccttg
    15001 ccgttgacgc cgtacatgcc gagggtgccc gtgagcgagc ggcggttgtt cagcagcagc
    15061 gggtacgaca ggcccagccc gaggcggtcg ttggtgacgg ttcgccgaaa gcccaggctt
    15121 tccacggccg cgtcgtgcgg ctgggcctca tagtgatagc catcgacctt cacggccagg
    15181 ccgtcgtggc cgatgggcac gtgcacctcg ccggcgtagt accgggcatc ctggccattg
    15241 agcgggatgg ctcccgtcaa cttgacctgc tcgcccaggg gcgtgaggct gttggtgccg
    15301 acgctggcca ggggctgcag gcccatgccc aggtcggaca cgccgcccag cacctgcacg
    15361 gggcggcgcg tggccgccac gctgagctcg gtggcgccgt cggcccggcg gggcaggtcg
    15421 agattggggg tgatggtcac gcctggcacg gtgcgcatca ggttgagcac gcgctcgagc
    15481 gtcgcctggg tcagcggttt ttccgtatag agcggctggg cgatggcttc cagccgcgcc
    15541 ctggccgcgc cgatgtcgcc ggtgacatcc accttgtcga tatagccttc gaccaccgtc
    15601 accaccacca ggccgttttc gaagttctgg ttctgcagca gggcgaacga caggggatag
    15661 ccgcgcgagc ggtacatctc ggtgatcttg ttgacctgct gcaccaggtc gcccacggtg
    15721 gtttccttgc cggccagcgg ggtcagcatg ccgctgactt ctgcaaaggg cagggcgtgc
    15781 acgcccgaca cgtcgaaatg gctcggcacg atgcgctggg ccaggcgggc ttgcacggcc
    15841 tgctgctcgg gcgtgggaat gggcgcgacc gtcggcgcgg gcgccgatgg ctgggagggg
    15901 cggcctatgg acggcaggga ttcgaccgga ttgccgcgca agggcgcgtc ggcatgcgcg
    15961 gccggagcgt tcagtaaaag gcaggacgcc agggcggaga aggaaacgcc gggaagaaaa
    16021 cgggtgcgcg cgattttcat gggctattta tatgcagagt cgccgagata atcattctgc
    16081 gcgaaatctt gccagcaaaa agccgggtgc gggcaggttt ttattttata tgtgggcaat
    16141 tattctcatt tttattatta acctgcagga gaaagttaaa gaattgcaaa gtcgtaatgt
    16201 ttaagacttt ttaagggaga taatcgcctg caagagagct tgaagattat tagcaatttt
    16261 gcgagcttgc gacataaaag ctaacgtaac agttgagcct atatggggta aaccctgatg
    16321 cgctaaaagt gcctgtgaca gagacttctc tagcatttgg gcgggagaaa cggattatct
    16381 gaatatacat tccgtgccaa ttttggcgat taatgtattt tcaaaatctt tcgtgctgca
    16441 ttttttgcag ataatcctcc cgcatcaggg cggtcctcgg acttctttag acgctcgggc
    16501 gtcgaattaa tggctgcggc agggcttgcg cccgactccg gccaaggtat gcatcgacgc
    16561 aagtcgacgc ttatgtggtt tgacaacgca ttaacgggta tgtcccggga ggttcaccat
    16621 gctgacccaa gtcaaaaact tctggaatga cgagtcgggc gccacggcca tcgaatatgg
    16681 cttgatcgcc ggtctcatcg cggtagtgat catcggtgcg ctgaccgtgc tgggcacgag
    16741 cctgaccgat ttgttcacgc gtgtgagcga cgcggtcggt agtgccggca agtcgtaagc
    16801 agttgggcgt ctgaactctc tttacgcgac ggccactgat cacggttgca gcgatgtgac
    16861 ctgatgccag tgttctcggt aaaccgggaa gcgcgtgtag atgcgcaagt gctggagtcg
    16921 cctctttgtt gttgttgagg gataccctgt ggtgggctgc ctacatcgcc ttcgggctct
    16981 gggtcgcata cagcgacttc cggatgcgcc gcgtacccaa tcacctgctg gtgattgcgg
    17041 ccgcggcaca ggggttatgg ctgctcgggt tctcgctggg tggcacgctg ccttccgtgg
    17101 gcgcgcaggg ctggcgcgac gcgctgccgg ccttcgcgct gggcctgtgc ttcgccgttt
    17161 gctggaagca gcggctcatg ggcgcgggcg acgtgaagtt cctggccgtg cagggcctgc
    17221 tgctgggcat caagccctgg ttcgcgatgc tgctgctggg ttccgtgctc agcggcgcgc
    17281 acgcgctgat gcaagtcctt tccaagcgcc gcggcgtggc gccgcggcag cgaggcgggc
    17341 cgtatgcggc ctatctggcg ctggcggcca tcgccagcgt ggcgtttatg ccgtcgaatt
    17401 cgcccttgtg ttcctggtgt tcttctgtct ggtctacgcg atactgacct acggcgtcat
    17461 catggcggcg cagcagtcca tcaactttgc cgccgaagag ggcgcgcgcc gcgtcctgca
    17521 gtgggcgagc gaccgcccca cgcgggccaa tgccgcgctc accctggccg cgtccacgtc
    17581 cgcctgggtg agcggcctgg gcggcatggc ggctacgcag atcgccgtgt gcgatcagag
    17641 cggcacgctc acttccaaca atgccaacgc cgccacgctg tgcaacggcc tggtgtccac
    17701 cagcgacgcc agccgcgtgg ccgtcgtggt gcgctatccc tatcaggcaa accctttggt
    17761 gccgcttctg ccgggcatca gctacctggt gccgaccatg ctgggcgcgc aggcctccgt
    17821 gcagttggcc gacgcgggcg gacagggcgg atgatgcggc gcgccggtcc aagccatgcc
    17881 gggagccatg cgtgtgcggc gcgccgtcgg caatcgggcg tggcggcgct cgaattcgcg
    17941 ctgtccatca ccgtcatgct gatggtggtg gtgggcatcg tcggtttcgg cgccctgttc
    18001 tgggcccagc aaaaattgtc caaggcggcg ggcgagggcg cgcaggccgc gctctatgcc
    18061 agcctgaccg gccaggccgt caccccggag caattgcagc cgctgctgca gacggcggcc
    18121 tgtaccgcgg tgcagcgcga agcgaacctg ctcattccca gcggctccac cgcgctggca
    18181 tcgggcagcg gctgttcggt gcagaccgcg ctgtgctcgg ccctgcctaa cggttggagt
    18241 ccggcggcgg gcgatgcgcc cgccatgtgc gccagcgtga accttcaata ttcgttaagc
    18301 aattggcctt tggtgtcatt gatggctacg ttggccggaa gtccgctttt tcgtggggca
    18361 aactggtttc ccacgcagct tgcggcacag gcggtcgtgc aagtgacgcc gtcggccgta
    18421 cagtccgcgt catcttcttg aaggcaggag ttccattgtg agtcgcgtta ccgcgcagat
    18481 agccgctgtt ttgctgattg tgatcgccgt ggtcctgggc gtcctggcct ggcgcctgtc
    18541 gcaacggcca cccgcgccgc cggcgccggt ggtcatcgcc tcgaccagga acgaaccgtc
    18601 ccgtcccacg gtgcccgtcg tcaccgccgc caaacccatc gcgtccggtt cgcgcatcga
    18661 agccggcgcc ctcacggtgg aacagtggcc gatgcgcccg gcgcagggac atgccacgct
    18721 cgagggactg gtggggcagt acgtgcggct cgacatctcc caagggcagc ccattaccga
    18781 cgccttgctg gtcaaaggcc tgtccacgca cctgaagccg ggcgagcgcg ccgtgacaat
    18841 cccggtcgat gaagtgtcgg gcgtgcagaa ccgcgtgcag ccgggcgata cggtggatct
    18901 tttctacgtg gtcgaacgcg gcagcgaagt cccgggatcg cagacccgcc tgctgcaggc
    18961 gcgcgtgccg gtgctggctt acggcatgca atcggtcgat ggcgcgccgc cgaccgagga
    19021 caacggcaag ccgtctgcgt cccgcaacaa cgcgccgccg cccgcgccgc gcaacgccgt
    19081 attggccgtc ccggtggaaa aggtcaatga gctgttgctg gccgcccgtt ccggccgcct
    19141 gatgatggcg ctgcgttcgc cgcaagacac cgacgttccc gatcccacgc tctttacgcc
    19201 gcgccaggcc gtgctgattg cgcgtcccga cctcacgcag gagcaaaaag atcacctgaa
    19261 agatccgatc aactcggcct atgccggcga cgatctgccg caactggccg gtcccgccac
    19321 gcccaagccc gacgcgcagc ccaaacccaa gcccatcaag ccgcgcggca agggcgccgg
    19381 gggcagcatc gaagtgctgc gcgggtccga aacgcaaacg gtgcattact gatgatgtcg
    19441 tccccccctt ccgctatccg gcaagagagc cccatgagcg ctgcccacca cggtatcggc
    19501 cgttccctgc aactgcttgg cctgggtctg agcctggcct tggccgccat gccggcctgg
    19561 gcgcagcagc gggccgcccg gcctgcccgg gccgtgccgg ccaaggccgc ggtggtgtcc
    19621 gagatccctt cggcgccgca gggtctcgaa gtgcccatca atggccaact gcccctgccg
    19681 ctgaccgcca cgcccacgcg tgtggcggtt gccgatcccg ccgtggccga tgtcaaggtc
    19741 ctggcgccca gcggcgggcg tcccggctcg atcatgctgc tcggccgcaa gatgggcgcc
    19801 accgaggtgc gggtctggac gggcaactcg cgcgagccgc aagtctggaa ggtgcaggtc
    19861 gtggccggcc tgaccaatac cggcggcgtg gtgcaggtgg acgtgaaggt cgtcgaattg
    19921 tccaacacgg tcatgaagga ggtcggcctg aactggaacg gacgcagcgg tccctggtcg
    19981 gcgggggccg gcaccggcac cggcagcgca ctgatccagg ccggcggctt cgtgggcgac
    20041 ctgggctata gcatcggcaa gttcaccgcg gacctcaacc tgctgcagac caacggcctg
    20101 gcccgcgtgc tggccgagcc gtcgctggtg gccctgtcgg gccagagcgc gagcttcctg
    20161 gccggcggcg agatccccat tcccgagtcg ggcggcctgg gcacgcagaa cgtcgtgttc
    20221 aagcccttcg gcatcggcct gacggtgtcg cctacggtca tctccaggga ccgcatcgcg
    20281 ctcaaggtgg cgcccgaggc cagcgagctc gactacagca acggcatcaa cctggtcaat
    20341 ggcaccacca ccacgatcat cccggcgctg cgcacccgca aggccgacac catgatcgaa
    20401 ctgggcgatg gcgagagcta catcatcagc ggcctggttt cgcgcgagac catggccaac
    20461 gtcaacaagg tgccgttcct gggcgacctg cccgtcatcg gcacgttctt caagaacgtg
    20521 cagtattccc agagcgacaa ggagctcgtc atcgtcgtga cgccgcacct ggtgcagccg
    20581 atcgcgcgcg gcgtggcgct gcccatgccc ggcggccgcc aggagcagag cgacgggtcg
    20641 accacggcct ggggctacta cctgttggga ccggccggca acgaacagat gccagggttc
    20701 tcgcggtgaa ttcgacgtcc atgaatacat ctgccaaaga agggctgact ttccaggcgg
    20761 cgcagttcct gttctgctcc gaggacaatg ccgtggccgc ggcgctgggc caggtgctgg
    20821 gcggcctggg cttgctggtg caagaggtgc cggcgctgga caccgtttcg cgccggttgg
    20881 gcgagcttgc gcccagcgtg gtgtttcttg atttcacggc cgatccggtc gatgtcgacc
    20941 agctcatgcg ctcgtccgac ctggcgcgcg tgctggcgcg cgtgtcgccc aatttgccgc
    21001 gcgtcgcggt gggctattcc tcgcgtccgg tgggcgccat cgcggcgctg cgggccggcg
    21061 tgtcggactt catcgacctg agcggcaagc ccgaagacgc cgtggacgtg gtgcagcact
    21121 tgctggcttt ccccggcgag gcgagcgcgg gcagcggcgc gcagcgcagc gtgctgatgc
    21181 tgggcgcgcg gtcgggcgtg ggggtgagca ccctggccgt gcatgcggcc agtttcacgc
    21241 agatgcgcct gcatcgctgg catgagcagc gcacgggaca gcccccgctc aagcagggca
    21301 agggcgagca ggaaatgctg ccgctcaccg agcgggtcag cttgctcgac atcggctacc
    21361 cggtgggcga ttgcctgctg tatctcaacc tcaccagcga tttcgacttc gccgaggcgg
    21421 cccgcaacat caacaggctc gatggcacca tgctcagcgc cgccatggcg cacaccgcgt
    21481 ccggcatcaa cgtcatgtcc ttgccgcgcg acctgacgca gctgcgcgac ctgtcgttcg
    21541 ccgattcact gtccctgtac gagcggctgc gccagtacag cggcatcatg ctgatcgacg
    21601 ccggcggcca cgagaacatc gagttcattt cgcagctggg gcgtgcctgc tcggacatct
    21661 ggctcgtcac cgaccagagc gtgagcgcgc tcgtcgcgct cgacagcctg gtcgccgagc
    21721 tgggcaagca gggcatagcg tgcgagcgga tgcgcctggt ggtcaaccga tacgacgaac
    21781 gctacggcat ggatgccggg cagatcgccc agcgcttcaa gctgccgctg gccggcacgc
    21841 tgccggatcg caccctgtcg ctcatgactt gcatgaacca gggccgcctg ctgcacgaag
    21901 tgaccgatcg cgacccctac gtgaagtccg tgcgctacct ggttggcaat ctcatggacg
    21961 agcgcttcgt gcagcccgcg cggccccaag gcaagctgtc cgcctggatg cacggctccc
    22021 gtcgccgttc ggccagcttc taaggaggag tcatgatcgc accctcgatg gagttcgggt
    22081 ccggcaacaa cagcaacgag tttgcgtcga gccagcgttt cctggacatc aagaaccagg
    22141 cctacgaaca cctgctctcg cgcatcgaag agctcggcgc cgaattcggc cgctggacgc
    22201 gcga
//
LOCUS       DASWPO010000350         7751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_262626, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000350 DASWPO010000000
VERSION     DASWPO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262626"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /gene="rrf"
                     /locus_tag="VGC69_18765"
     rRNA            <1..110
                     /gene="rrf"
                     /locus_tag="VGC69_18765"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..1726
                     /locus_tag="VGC69_18770"
     CDS             275..1726
                     /locus_tag="VGC69_18770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0297377.1"
                     /translation="MTSKDGLRWCRRLIAPDGEQPRHIPPLIIGASVFFACLFGIYTR
                     PVGFLATIWPANAIMLGILLRMRASASAPGWLAAGVAYLAADLLTGSTLLKALLLNGA
                     NLCGVATGYLICSRLSLDMLRLRQPASILYIVFAAAAAAAVAGVVGAIANPILFGGSV
                     ASGWTFWFATELANYIAILPVMLAAPAAGRWAAARMQLRSSVSGHAAIPIVAFALSCL
                     VATWVGGPGAIAFPVPALLWCGLAYSVFPTAVLTLLFGCWSLSIVSALYLSSPAGIVD
                     ESALVSIRLGASLIALAPIMLASVMESRNDLLARLHDLANHDPLTGADNRHAFREGAQ
                     RLFADQAWPVAVLAIDLDHFKIVNDTYGHPVGDEVLVAFAERVRRCLGVSDLLGRVGG
                     EEFAVMVPDCPMPDAAGLAQRLLEAIGGTPIILADKRAITITASIGIAVALDTDAISL
                     DKVLADADAALYRAKAKGRNRADISPGDREASR"
     gene            complement(1723..2673)
                     /locus_tag="VGC69_18775"
     CDS             complement(1723..2673)
                     /locus_tag="VGC69_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY0297378.1"
                     /translation="MNIHKHARLTFIRRLEMVQQLTDKQLTVPQAASAYGVTAPTVRK
                     WLGRFLAQGQTALADASSRPAVSPRAIAPAKALAIVELRRKRLTQARIAQALGVSAST
                     VSRVLARAGLSRLADLEPPEPVGRYEHEAPGDLLHIDIKKLGRIVRPSHRVTGNRRDG
                     VDGAGWDFVFVAIDDHARVAFTDIHPDECFPSAVQFLKNAVLYYERLGVTIRRLLTDN
                     GSAFRSHAFAALCRELGIKHRFTRPYRPQTNGKAERFIQSALREWAYAYTYQNSQHRA
                     DAMKSWLHHYNWHRPHQGIGRATPISRLKLDEYNLLTLHT"
     gene            complement(2784..3407)
                     /locus_tag="VGC69_18780"
     CDS             complement(2784..3407)
                     /locus_tag="VGC69_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0297379.1"
                     /translation="MAGVRQFNEDQVFDSALALFWDKGYTDTTMQDLAAATGVQRGSL
                     YHAYQDKETLFLRVFERYRSRFLEQVETALASPDLYTALRGFIDLLITSMLTGQPTRG
                     CLSTKTALSNDVFHEPIRHALQGILDGYSRLLRQRLARPDAAQGLALPAEQAIELILT
                     FTRGLVVIERVYQDPERLRASSETLLSVLLKKPGQGPTNRKRPANPS"
     gene            complement(3444..3599)
                     /locus_tag="VGC69_18785"
     CDS             complement(3444..3599)
                     /locus_tag="VGC69_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297380.1"
                     /translation="MKTVAMDTPSGALCADGRAYPRALAGKSIFDTHVIFDMLVNIDT
                     NDKNGVK"
     gene            3612..4823
                     /locus_tag="VGC69_18790"
     CDS             3612..4823
                     /locus_tag="VGC69_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEY0297381.1"
                     /translation="MTTAPAGAGASGAALLRYLFAGFCASLVTIGLARFVYTPLLPVL
                     IQAHWFTASQAVYLGAANLAGYLAGALIGRATGRRLGNVAALRGMMLVAALSFFACAV
                     PVSVAWFFAWRLVSGVAGGVVMVLIAATVLPHVPPARRGMAGGAIFVGAGLGIAASGT
                     LVPLLLDYGLQATWMGLGVLSAVLCAASWYAWPPMSATAAAAAMPSGAARAVHGAGVT
                     TLYVEYAFMAAGFVPGMVFLVDFVARGLGAGAHLGGLFWVVYGVGAIIGPALYGWLID
                     RLGVRATVRWVLLSQAVAMAGLYRVQGYAGIGALALVLGMFPAGLPPMILARLQQLIP
                     HDIPAQGLAWSRATIIFATLQAAAGYGYSMLFDASAGNHRLMFLTGAAWTLAALLLDL
                     APAWPAHAKAR"
     gene            complement(4890..5444)
                     /locus_tag="VGC69_18795"
     CDS             complement(4890..5444)
                     /locus_tag="VGC69_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY0297382.1"
                     /translation="MAKTVVVFFSGYGHTQRVAESVAQGAGAELIAIDAEGNLSDDAW
                     TTLDSADAIIFGAPTYMGSVPWQFKKFADASSKKWFVRAWQDKVFGGFTNSASLNGDK
                     QVTLITLQTLASQHGGLWVSLGLPPANKLASTRDDVNSLGGSVGALAQSPSDAGADAI
                     SSGDLETAKHYGARVAAIAKRLVG"
     gene            complement(5547..6251)
                     /locus_tag="VGC69_18800"
     CDS             complement(5547..6251)
                     /locus_tag="VGC69_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0297383.1"
                     /translation="MSDLSKFPITRKWPARHPDLIQLYSLPTPNGVKVSIMLEETGLP
                     YEAHRVDFATNYQTTPEFLSLSPNNKIPAILDPNGPDGKPFGLFESGAILVYLAGKAG
                     QFIPADTAGRYETLQWLMFQMGGIGPMFGQLGFFNKFAGKDIEDKRPRDRYVAESKRL
                     LGVLDRHLAKRDWIMGRAYTIADMATFPWVRNLIGFYEAGDLVGIADFPNVTRVLEAF
                     LARPAVARGLAIPAAA"
     gene            complement(6318..6722)
                     /locus_tag="VGC69_18805"
     CDS             complement(6318..6722)
                     /locus_tag="VGC69_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEY0297384.1"
                     /translation="MSRPSAESSACPCGTGLAYAACCGRWHRGAQCLQAPTAEALMRS
                     RYTAFTQDGLDYLLQTWHPDTRPAGLDPNPPGLKWLGLRIVAQAQQDPTHATVEFVAR
                     YRQAGKATRLHETSRFVRLDGRWLYLDGEFPA"
     gene            complement(6738..7010)
                     /locus_tag="VGC69_18810"
     CDS             complement(6738..7010)
                     /locus_tag="VGC69_18810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0297385.1"
                     /translation="MTDIIHHATQGRFESMVDGHLCELDYRLDGNRMAILHTGVPPQV
                     GGRGIAADLTRAALDLARSRGWQVLPLCSYAAAYLRRHPEYADLLG"
     gene            complement(7007..>7751)
                     /locus_tag="VGC69_18815"
     CDS             complement(7007..>7751)
                     /locus_tag="VGC69_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297386.1"
                     /translation="LIEGNVYGWTSQPILAAAAVAAISFVLVVKRERSHASPVLPRSL
                     YAMRGFGAANAIGFLINFGSFGQLFLLSLYLQQSQGASALAAGVQLLPMMGVFTIGNL
                     LSSRITARLSLRVTLVGGLVLSVIGGAAATLTGAQSYGSFVAWVALCNLGIGVAIPAM
                     TATTMRLAGRAHANGAAAALNANRQAGALVGVAVMGMLIHGLAGWPERLVLAFGIGAA
                     GYAIGALLAWRRLGYPAGTTTSPAPRTPS"
BASE COUNT         1184 a         2318 c         2770 g         1479 t
ORIGIN      
        1 gacgaccata gcgtctcggt accacccctt cccttcccga acaggaccgt gaaacgagac
       61 cgcgccgatg atagtggatg gacatctgtg aaagtaggtc atcgtcaggc tcttatccta
      121 aaaagcccac ctccctcgag gtgggctttt tacctttgtg gacggcagga ttccagccac
      181 ggtttggctc ggatgccttc gtcgcccgta gttgatggca aggatggtat ttgggggtac
      241 gatctccggt ctgccttgct ggtgggagac ggtcatgacc tcgaaggacg gcttgcgctg
      301 gtgccgaaga ctcatcgccc cagacggcga acagccaagg catatcccac cgctaataat
      361 cggcgcctcg gtttttttcg cttgcctgtt cggcatctac actcgcccgg tcggttttct
      421 cgctacgatt tggccggcca acgccatcat gctgggcata ctgcttcgca tgcgcgcatc
      481 ggcaagtgcg ccagggtggc tggctgccgg cgtggcgtac cttgccgcag accttcttac
      541 cggatccacg ctactcaagg ccctgctcct gaacggcgca aatctctgtg gcgtggcgac
      601 gggctacctg atttgctcgc ggctctccct tgacatgctc cggttgcggc aaccggcgtc
      661 aatactctat atcgtcttcg ccgcggccgc ggctgccgcc gtcgccggcg tggtcggcgc
      721 gattgccaat cccatcctgt tcggtggcag cgtcgccagc ggctggactt tctggttcgc
      781 gacggagctc gccaattaca tcgcgatatt gcccgtcatg cttgcggcgc cggccgccgg
      841 caggtgggcg gccgcacgga tgcaactgcg ctcgtcggtt tccgggcacg cagccattcc
      901 gatcgtggcg ttcgccttgt cctgcctggt cgcgacctgg gtcggggggc cgggcgccat
      961 cgcctttccc gtccctgcgc tactgtggtg cggcttggct tattccgtat tcccgacggc
     1021 cgtcctgacg ctgcttttcg gttgctggtc cctgagcatc gtttccgccc tttatttatc
     1081 cagtcccgcg ggcatcgtcg acgagagtgc gctcgtgtcg atcaggctgg gagcctccct
     1141 catcgcactt gcccccatca tgcttgccag cgtcatggag agccgcaacg acttgctggc
     1201 gaggttgcat gatctagcca accatgaccc attgaccggg gcggacaacc gccatgcctt
     1261 ccgggaaggc gcccagcggc tgtttgccga ccaggcatgg cccgttgccg tgctggctat
     1321 cgacctggat catttcaaga tcgtcaacga tacgtatggg catcctgtcg gcgacgaggt
     1381 tctcgtggca tttgccgagc gggtcaggcg ctgtctgggc gtctccgatc tgctggggcg
     1441 ggtaggcggc gaggagttcg cggtgatggt gcccgattgc ccgatgccgg atgcggcagg
     1501 cctcgcacaa cggcttctgg aggcgatcgg agggacgccg atcattctcg ccgacaaacg
     1561 tgcgataacg atcaccgcca gtatcggcat agccgtcgcg ctggatacgg atgcaatctc
     1621 cctcgataaa gttctcgcgg atgccgatgc tgcgctttac cgcgccaagg cgaaagggcg
     1681 caacagggcg gatatctcac ccggggatcg ggaagcttcg cgctaggtgt gaagagtcaa
     1741 gaggttgtat tcatccagtt tgagtctgga gatgggtgtg gcgcgcccga tgccctggtg
     1801 agggcgatgc cagttgtaat ggtgtagcca ggatttcatg gcatcggcac ggtgctggga
     1861 gttttggtag gtgtaggcat aggcccactc acgcaaggcc gattggatga atcgttcggc
     1921 cttgccattg gtctgtggcc ggtagggccg ggtgaagcga tgcttgatgc cgagttcgcg
     1981 gcatagcgcg gcgaaggcgt ggctgcggaa ggccgagccg ttgtcggtga gcagtcgccg
     2041 gatggtcacg cccaggcgtt cgtagtagag cacggcgttc ttcaagaact ggactgcgct
     2101 ggggaagcac tcgtcggggt ggatatcggt gaaggccaca cgggcgtggt catcaatggc
     2161 cacgaagacg aagtcccagc cggcaccgtc cacaccgtcg cggcggttgc cagtcacccg
     2221 gtggctcggg cgcacgatgc gcccgagctt cttgatgtcg atgtgcagca aatcaccggg
     2281 ggcctcatgt tcgtagcgcc ccaccggctc gggcggctcc agatcggcca ggcgcgacag
     2341 gccagcgcgg gccagtactc ggctgaccgt gctggccgac acgcccaggg cctgggcaat
     2401 gcgggcctgc gtcaggcgct tgcggcgaag ctcgacgatg gccagcgcct tggccggcgc
     2461 aatggcccga ggcgagacgg ccgggcgcga ggatgcatcg gccagggctg tctggccctg
     2521 ggccaggaag cggcccagcc acttgcgtac cgtcggtgcc gtcaccccgt aggcagaagc
     2581 tgcctggggc accgtgagct gtttgtcggt taactgctgg accatttcga gtcgacgtat
     2641 gaacgtcagt cgggcatgct tatgaatgtt catccggctg ggcctcctga gaatcctggg
     2701 tgtttggcga cttccagttt ctcaaacccg gttcggatga accatgcata caacctattg
     2761 aatcttcaca gctaggccac gcgtcaggac gggttcgcgg gccgcttacg attcgtcggg
     2821 ccttgtcccg gcttcttcag aagcaccgag agcagggttt ccgaactggc gcgcaggcgt
     2881 tcgggatcct ggtagacgcg ttcgatgacg accaggccgc gtgtgaacgt gaggatcagt
     2941 tcgatggcct gctcggcggg cagggccagg ccttgggcgg catcgggccg ggccaggcgc
     3001 tgcctgagca gccggctgta gccgtcgagt atgccttgca aggcgtggcg gatgggttcg
     3061 tggaagacgt cattgctcag ggccgtcttg gtggacaggc agccccgcgt gggctggccg
     3121 gtgagcatgg acgtgatgag caggtcgatg aaaccgcgca aggccgtgta caggtcgggc
     3181 gaggccagcg cggtttccac ctgctccaga aagcggctgc gatagcgttc gaagacgcgc
     3241 aggaacagcg tctccttgtc ctgataggca tggtagagcg aaccgcgctg cacaccggtg
     3301 gccgcggcca ggtcctgcat ggtggtgtcg gtgtagccct tgtcccagaa gagggccagg
     3361 gcggagtcga agacctggtc ttcgttgaac tgacgtacgc ctgccatggt gcggtgttgc
     3421 ttccttgcat gaatttcctg tgcttatttg acgccattct tgtcattcgt gtcaatattg
     3481 actagcatgt caaaaatgac atgagtgtca aagatgctct tgccagcaag agcgcgcgga
     3541 taggcccggc cgtctgcgca taacgcgccc gagggcgtat ccatggcaac ggttttcata
     3601 tgtccacctc catgacaacg gcgcctgcgg gcgcaggcgc atccggcgcg gccttgctgc
     3661 gctacctgtt cgccggtttt tgcgccagcc tggtcacgat aggcttggcg cgtttcgtct
     3721 acacgcccct gctgccggta ttgatccagg cgcactggtt cactgcgtcc caggcggttt
     3781 acctgggcgc ggccaacctg gccggttatc tcgccggcgc gttgatcggc cgcgcaacgg
     3841 gacgccgcct gggcaatgtc gcggccctgc ggggcatgat gctggtggcc gcgctgtctt
     3901 tcttcgcctg tgccgtgccg gtttcggttg cctggttctt tgcctggcgg ctggtgtcgg
     3961 gcgtggcggg tggcgtggtg atggtgctga ttgccgccac ggtgctgccg catgtgccgc
     4021 ctgcccgccg gggcatggca ggcggggcga ttttcgtcgg cgcgggcctg gggatcgcgg
     4081 cttccggcac cttggtgccg ctgctgctgg attacggcct gcaggcgacc tggatggggt
     4141 tgggcgtgct gtccgccgtg ttgtgcgcgg caagctggta tgcctggccg cccatgagcg
     4201 cgaccgctgc cgcggcagcg atgccttccg gcgcggcgcg ggcggtacac ggcgccggcg
     4261 tgacgacgct gtatgtcgag tatgcgttca tggccgcggg gttcgtgccg ggcatggtgt
     4321 ttctggtgga tttcgtcgcc cgcggcctgg gggccggtgc gcacctggga ggactattct
     4381 gggtcgtgta cggggtgggc gccatcatcg gcccggccct gtacggctgg ctgatcgatc
     4441 gcctcggcgt gcgggccacg gtgcgctggg tgctgctgtc gcaggcagtg gcgatggcgg
     4501 gcctgtatcg ggtccagggc tatgccggca tcggcgcgct ggccctggtg ctggggatgt
     4561 ttcccgcagg gttgccaccc atgatcctgg cccgcctgca gcagttgatt ccgcacgaca
     4621 tcccggcgca gggcttggcc tggagccggg cgacgatcat tttcgcgact ctgcaggccg
     4681 cggcgggata tgggtattcg atgctgttcg acgcgagcgc cggcaatcac cgcctgatgt
     4741 tcctgacggg tgccgcctgg acgctggccg cgctgcttct ggatcttgcg cctgcctggc
     4801 cggcgcatgc aaaggcccgc taaagagcgg gcctctgggg ggaaatccgc cattgccctg
     4861 tggcgatgac ggatggtctt gccgccggat cagccgacca ggcgcttggc gatggcggcc
     4921 acgcgggcgc cgtaatgctt ggcggtttcg agatcgccgg acgagatggc atcggcgccg
     4981 gcgtccgacg gcgactgcgc cagcgcgccg accgagccgc ccaggctgtt gacgtcgtca
     5041 cgggtggacg cgagcttgtt cgccgggggc aggcccaggc tgacccagag gccgccgtgc
     5101 tgcgaggcca gcgtctgcag ggtgatcagg gtgacctgct tgtcgccgtt gaggctggcg
     5161 ctgttggtga agccgccgaa gaccttgtcc tgccaggcgc ggacgaacca tttcttggag
     5221 ctggcgtcgg caaacttctt gaactgccag ggcaccgagc ccatataggt cggcgcgccg
     5281 aagatgatgg cgtcggcgct gtccagcgtg gtccaggcgt cgtcgctcag attgccttcg
     5341 gcgtcgatgg cgatcagttc ggcaccggcg ccttgggcga cgctttcagc gacgcgttgg
     5401 gtgtggccgt agccggagaa gaaaacaaca acagttttcg ccatgagaaa actccttgag
     5461 aatgaggcgg ccgtaaaaaa taggccagcc tcggactcta cgcgtcttgc tgccgcgcaa
     5521 caatcgacat ggatggaaca gtttgttcag gcagcggcgg ggatggccag cccgcgggcc
     5581 acggccggcc gggccaggaa ggcctcgagc acgcgggtca cgttgggaaa gtccgcgatg
     5641 ccgaccagat cgcccgcctc gtagaagccg atgaggttgc gcacccaggg gaaggtggcc
     5701 atgtcggcga tggtgtaggc gcggcccatg atccagtcgc gcttggccag gtgccggtcg
     5761 agcacgccca gcaggcgctt ggattcggcc acgtagcggt cgcgcgggcg cttgtcttcg
     5821 atatccttgc cggcgaattt gttgaagaag ccgagctggc cgaacatggg gccgatgccg
     5881 cccatctgga acatcagcca ctgcagggtt tcgtagcggc cggccgtgtc cgccgggatg
     5941 aactgcccgg ccttgcctgc caggtagacc aggatggcgc cggattcgaa cagcccgaag
     6001 ggtttgccgt cggggccgtt ggggtcgagg atggcgggga tcttgttgtt ggggctgagc
     6061 gagaggaact cgggggtggt ctggtagttg gtggcgaagt ccacgcgatg ggcttcatag
     6121 ggcaggccgg tttcttccag catgatggag accttcacgc cattgggcgt gggcagcgag
     6181 tacagctgga tgaggtcggg atggcgcgcg ggccacttgc gcgtgatggg aaatttcgac
     6241 agatcgctca tgggatcaaa gacctggagg ttgggatggc ggaaggcgcg ggccttgcac
     6301 aggtccgcgg ggggatctta tgccggaaat tcgccgtcga ggtagagcca gcggccgtcc
     6361 agcctgacga agcggctggt ttcgtgcaat cgggtggctt tgccggcctg gcgatagcgc
     6421 gccacgaatt cgaccgtggc atgggtgggg tcctgctgcg cctgggccac gatgcgcagg
     6481 cccagccact tgaggccggg cggattgggg tcgaggccgg cgggccgcgt gtcgggatgc
     6541 caggtctgca gcaggtagtc gagcccgtcc tgcgtgaagg cggtgtagcg cgagcgcatc
     6601 agggcctcgg ccgtgggggc ttgcaggcat tgcgcgcctc gatgccagcg gccgcagcat
     6661 gcggcgtagg ccaggccggt gccgcagggg caggcgctgg attcagcgga tggccggctc
     6721 atcgacacgg gccgttttca gccgagcagg tcggcgtatt cgggatggcg gcggaggtag
     6781 gccgcggcat aggagcagag tggcagcacc tgccagcccc ggctgcgcgc caggtcgagc
     6841 gccgcgcggg tgaggtcggc ggcgatgccg cgtccgccca cctggggcgg cacgccggtg
     6901 tgcaggatgg ccatgcgatt gccgtcgagg cggtagtcga gttcgcagag atggccgtcg
     6961 accatggact cgaatcggcc ttgcgttgcg tggtggatga tgtccgtcat gaaggggtgc
     7021 ggggtgcggg gctggtggtc gtgcctgcag gataacccag ccgccgccat gccagcagcg
     7081 cgccgatggc gtagcccgcc gcgccgatgc cgaaggccag gaccaggcgt tccggccagc
     7141 cggccaggcc gtgtatcagc atgcccatca cggcgacgcc gaccagcgcg ccggcctggc
     7201 ggttggcatt gagcgccgcc gcggcgccgt tggcgtgggc gcgaccggcg agccgcatgg
     7261 tggtggccgt catggccggg atggccacgc cgatgcccag attgcacagt gcgacccagg
     7321 ccacgaagct gccatatgac tgtgcgccgg tcagggttgc cgcggcgccg ccgatcacgc
     7381 tcaagaccag gccgccgacc agcgtgacgc gcaggctcag gcgggccgtg atgcgcgacg
     7441 acagcaggtt gccgatggtg aacacgccca tcatgggcag cagctgcacg cccgcggcca
     7501 gggcgctggc gccttgcgac tgctgcagat agaggctcag caggaacaac tggccgaacg
     7561 agccgaagtt gatgagaaag ccgatggcat tggccgcgcc gaagccgcgc atggcgtaca
     7621 ggctcctggg cagcaccggg ctggcgtggc tgcgttcgcg cttgaccacc agcacgaagc
     7681 tgatggcggc cacggcggcg gcggccagga tgggctggct ggtccagcca tagacattgc
     7741 cttcgatcag g
//
LOCUS       DASWPO010000351         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_441209, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000351 DASWPO010000000
VERSION     DASWPO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441209"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VGC69_18820"
     CDS             <1..397
                     /locus_tag="VGC69_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0297387.1"
                     /translation="LSLKHELRVMTAQGSGSVINISSTYGHEGAAFASLYAGSKHAVE
                     GITKSAALEVAGTGVRVNAVAPGPTDTGMLVRFTGTPEKKAALAGKVPLARIGRPEDI
                     AAAVRYLASEGAAFVTGQILTVDGGKTAG"
     gene            418..1518
                     /locus_tag="VGC69_18825"
     CDS             418..1518
                     /locus_tag="VGC69_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY0297388.1"
                     /translation="MNNVLFTPLDLGRIRLGHRVVMAPLTRLRADRDGDMPNALMREH
                     YRQRASDGGLIITEATTISPTARGYLGAPGLYTDAQVAGWRAIVDAVHQRGGRILAQL
                     WHVGRTSHASLAGGAAPVTASAVPYEGLAFTTAGWTPVSPARALETGEIAGIVADYAA
                     AAQRARDAGFDGIEIHAANGYLLDQFIQDGSNRRDDRYGGSIENRTRLLMEVVQATRA
                     VWGGGRIGVRISPSSSFNGMSDSDPDAVFGHIAESLADQDLAYLHVVEPRVVGSEAVA
                     DGLQPVASRELRPRFGGPVIAAGGFAGAEAQTEISHGNVDAVAFGRHFIANPDLPVRL
                     RAGLALNPYNRATFYGGGAQGYTDYPAAALAA"
     gene            1539..2285
                     /locus_tag="VGC69_18830"
     CDS             1539..2285
                     /locus_tag="VGC69_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0297389.1"
                     /translation="MNALSGKTALVTGASRGMGRASALALAKAGTQVLVHYGKAAQEA
                     EAVVAEIRQAGGKAEKVAADLSLPDGPHELARQARAIIGPRLDILVANAGIAKAATIE
                     DTSVADFDELFAVNVRAPFFLVQQLLPAMCKGSSIVLLSSLAAHSVVGNLAAYAATKG
                     AIDTLVKHFAAALGEREIRVNAVAPGIVPTDMSNFAKTEAGRDFTHSIQALKRLAAPQ
                     DIADAVVFLASDQARWMTGDTLRVDGGSKL"
     gene            2358..2990
                     /locus_tag="VGC69_18835"
     CDS             2358..2990
                     /locus_tag="VGC69_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0297390.1"
                     /translation="MKLYHSSKSPNARRVRIFIAEKGISIPAQAVDLGAKEQFSPAYA
                     AINPRRQVPALVLDDGTTIAEVPAIWRFIEEAFPEHPLLGSTAKEKALVAMWERRAEL
                     DGFMPVMEGVRNAVPGLKGRALSGPHDYEQIPDLIERSRQRVANFFADFDERLRAVPF
                     VAGDLFSAADITTLAAIDFAAGGLDLAVPATHTALLDWYGRASARPSATA"
     gene            complement(3035..4039)
                     /locus_tag="VGC69_18840"
     CDS             complement(3035..4039)
                     /locus_tag="VGC69_18840"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297391.1"
                     /translation="MHSFRQRCPAILARHALAAIVALGGLSLALPAAAAAAYPDKPIT
                     VINPWTPGGPADTVARPIMDKLSQRLGKAIIIENRPGANGVIGAAYVARAAPDGYTLL
                     FSHVGPITISPATQKDMPYDSLRDFAPITQIVSAATVLLVRPDLPIHNLKELIDYARA
                     HPGKLSYGSVGPGSTTHLAGEILAHMADVKLLHVPYKGAAPVVTDLLSGQISMAFLNI
                     AGAMPYIKSGQLRPIGVSTLKRSTLLPDLPAIAQTYPGFEVNSWYGLMAPAGTPKPIV
                     DRLQTEIAEILKMPDIVKLLSFNGLAPEGTTPEVYTQQIRKDLDRWQAVVKQAGLQPQ
                     "
     gene            complement(4082..4861)
                     /locus_tag="VGC69_18845"
     CDS             complement(4082..4861)
                     /locus_tag="VGC69_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0297392.1"
                     /translation="MSGTVITEVRDGVAWLTLNRPEALNAINEAVRAELPAALAAADA
                     REDVRVVVLRGAGPRAFCVGADIKEFSAVGSALAWRQQRAHRHWISAFDRVVKPTIAA
                     VHGHCLGGGLEIALACDIRIAADDASFGFPETGHGIIPGAGGTQRITRLLGQGVALDL
                     VLTGERIDAARALAMGLVSRVAPAAGLAELAAAVAARIAERPPLAVQFAKEAVRAGAE
                     LPLAAGLRYETDLSAHLINTEDRLEAARAFREKRKPVFTGR"
     gene            complement(4861..5271)
                     /locus_tag="VGC69_18850"
     CDS             complement(4861..5271)
                     /locus_tag="VGC69_18850"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY0297393.1"
                     /translation="MSTSDKQAAAALEPTGYWQRTRELGFTLPRCEDCGSFHFYPRPA
                     CPACGSERVAPQPASGRGQVYSHSVVYRAPSPAYAADVPYVVAIVATDEGPHLMTRIV
                     GMDPEAVAIGLRVRVRADGEGREPVFEPDAAREA"
     gene            complement(5276..>5562)
                     /locus_tag="VGC69_18855"
     CDS             complement(5276..>5562)
                     /locus_tag="VGC69_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0297394.1"
                     /translation="VELEDLGFCAKGEGGAFVENGAIGLGGRLPVTTHGGLLSAGHPG
                     LAGGMFHVLEGVRQLRGQAGERQVAGAGTALVHGNGGIVGLHCSVILGKE"
BASE COUNT          907 a         1976 c         1849 g          830 t
ORIGIN      
        1 gctgagcctc aagcacgagc tgcgggtcat gaccgcgcaa ggcagcggca gcgtcatcaa
       61 catctcgtcg acctatggcc acgagggcgc ggccttcgcc tcgctctacg cgggcagcaa
      121 gcacgcggtc gagggcatca ccaagtcggc cgcgctcgaa gtcgccggaa ccggcgtgcg
      181 cgtcaatgcc gtcgcccccg ggccgaccga tacggggatg ctggtccgct tcaccggcac
      241 ccccgagaag aaggcggcgc tggccggcaa ggtgccgctg gcccgcatcg gcaggcccga
      301 agacatcgcg gccgcggtgc gctacctcgc ctccgaaggc gcggctttcg tcaccggcca
      361 gattctgacc gtcgacggcg gcaagaccgc cggctgacca ggagaaagga acacatcatg
      421 aacaacgtcc tcttcacccc tctagacctc ggccgcatcc ggctcggcca ccgggtcgtc
      481 atggcaccgc tgacccgcct gcgcgccgac cgggatggcg atatgcccaa cgccctgatg
      541 cgcgagcatt accgccagcg cgcctccgac ggcggcctca tcatcaccga agcgaccacg
      601 atctcgccaa cggcccgagg ctaccttgga gcgcccgggc tctacaccga cgcccaagtg
      661 gcaggctggc gcgccatcgt cgatgccgtg caccaacgcg gcggccggat cctggcccag
      721 ctctggcacg tcggccggac ctcgcacgca agcctggccg gcggcgcggc gccggtcacc
      781 gcctcggccg tcccgtatga aggactggct ttcacgaccg caggctggac ccccgtttcg
      841 ccggcgcgcg cgctggaaac cggcgagatc gccggcatcg tggccgacta cgcggcggcc
      901 gcgcaacggg cacgcgatgc cggtttcgat ggcattgaaa tccacgcggc caacggctat
      961 ctgctcgacc agttcattca ggacggcagc aatcgccgcg acgaccgcta cggcggctcg
     1021 atcgagaacc gcacccgcct gctgatggaa gtcgtgcagg ccacgcgggc cgtctggggc
     1081 ggcggccgga tcggggtgcg catttctccc agcagcagct tcaacggcat gtcggacagc
     1141 gaccccgacg ccgtcttcgg ccatatcgcc gagtcgctcg ccgatcagga cctggcttac
     1201 ctgcacgtcg tcgagccgcg cgtcgtcggg tcggaggcag tggccgatgg cctgcagccg
     1261 gtggcttcgc gcgagctgcg cccacgcttc ggcggcccgg tgatcgcggc cggcggattt
     1321 gcgggcgccg aagcgcaaac cgagatctcc catggcaacg tcgatgcggt ggccttcgga
     1381 cggcacttca tcgccaaccc cgacctgccg gtccggctgc gtgcggggct ggcgctcaac
     1441 ccctacaacc gcgccacctt ctacggtggc ggcgcgcagg gctatacgga ctaccccgcg
     1501 gccgcgctgg ccgcctgaaa ccaccaggag cacacatcat gaatgcactt tccggaaaga
     1561 cggcgctggt taccggcgca tcgcgcggca tgggacgagc cagcgccctg gcgctggcga
     1621 aggccggcac ccaggtactc gtccactacg gcaaggccgc ccaggaagcc gaggcggtcg
     1681 tcgccgaaat ccgccaggcg ggaggcaagg ccgaaaaggt cgccgccgac ctgagcctgc
     1741 cggacgggcc gcatgaattg gcccggcagg cgcgcgccat cattggccca cgcctggaca
     1801 tcctggtggc caatgccggc atcgccaagg ccgccacgat cgaggacacg agcgtcgcag
     1861 acttcgacga gctgttcgcg gtgaatgtcc gcgcgccgtt cttcctggtc cagcaactgc
     1921 tgcccgcgat gtgcaagggc agcagcatcg tgctgctctc gtcgctggcc gcgcactcgg
     1981 tcgtcggcaa tcttgccgcc tatgcggcca ccaagggcgc catcgatacg ctggtcaagc
     2041 atttcgccgc ggccctgggc gagcgcgaaa tccgcgtcaa cgccgtggcg ccgggcatcg
     2101 tgccgaccga tatgtccaac ttcgccaaga ccgaggcggg acgtgacttt acgcacagca
     2161 tccaggcgct caaacgcctg gccgcgccgc aagacatcgc cgacgccgtg gtgtttctcg
     2221 cctcggacca ggcgcgctgg atgaccggcg acacgttgcg cgtcgacggc ggctcaaagc
     2281 tttgaccccc gggaacgggc cacgcggcat ccgcggtcct ttcgattccc tccctcacat
     2341 tgaacggagc aatacccatg aaactctatc attccagcaa gtcgcccaat gcgcgccgcg
     2401 tgcgcatctt cattgccgag aaaggcattt cgatcccggc ccaggcggtc gatctcggcg
     2461 cgaaagaaca gttctcgccg gcttatgccg cgatcaaccc gcgccgccag gtcccggcgc
     2521 tggtattgga cgacggcacg acgatcgccg aggtgccggc gatctggcgc ttcatcgaag
     2581 aagcttttcc cgagcatccg ctgctgggtt cgacggcaaa ggaaaaggcg ctcgtcgcca
     2641 tgtgggagcg ccgcgccgag ctggacggct tcatgccggt catggaaggc gtacgcaacg
     2701 cggtcccggg gttgaagggc cgcgcgctct ccggaccgca cgactatgag cagattcccg
     2761 acctgatcga acgcagcagg cagcgcgtgg cgaacttctt cgccgacttc gacgaacggc
     2821 tgcgagccgt tcccttcgtg gctggcgacc tgttctccgc cgcggacatt acgacgcttg
     2881 cggcgatcga ctttgcggcg ggagggctgg accttgccgt cccggcaacg cataccgcgc
     2941 tgctggactg gtacggacgg gcaagcgcgc gcccgagcgc cacagcctga ttttgctgaa
     3001 acgcgcccag gcccgggaag gcctgtttcc aagcctactg cggctgcagg ccggcctgtt
     3061 tcaccaccgc ctgccaacgg tcgagatcct tgcggatctg ctgcgtgtac acctccggcg
     3121 tcgtcccttc cggggccaaa ccgttgaacg acagcagctt gacgatatcg ggcatcttca
     3181 atatttcggc gatctccgtc tgcagccggt cgacgatggg cttgggcgtg cccgccggcg
     3241 ccatcaggcc ataccaggaa ttgacttcga agccgggata ggtctgggca atggcaggca
     3301 ggtcgggcag caatgtcgag cgcttgagcg tgctcacccc gatgggccgc aactggccgg
     3361 acttgatgta gggcatcgcc ccggcaatat tcaagaaggc catgctgatc tgcccgctga
     3421 gcaagtcggt gacgaccggc gccgccccct tgtacggcac gtgcagcagc ttcacgtcgg
     3481 ccatgtgggc aaggatctcg cccgccaggt gggtcgtgct gcccggtccc accgatccgt
     3541 acgacagctt gccgggatgc gcccgggcat agtcgatcag ctctttcagg ttgtggatgg
     3601 gcaggtcggg acgcaccagc agcacggtcg ccgcggacac gatctgcgtg atcggcgcga
     3661 aatcgcgcag cgaatcgtag ggcatgtctt tttgcgtggc cgggctgatc gtgatgggcc
     3721 cgacatgcga gaacagcagc gtgtagccgt ccggcgcggc gcgcgcgacg taggccgcgc
     3781 cgatcacgcc attggcgccc gggcggttct cgatgatgat ggccttgccc aggcgctgcg
     3841 acagcttgtc catgatgggc ctggccaccg tgtcggccgg accgcccggc gtccacggat
     3901 tgatgacggt aatgggcttg tccggatagg ccgcggccgc agccgccggc aaggcaaggg
     3961 acagtccgcc cagtgccacg atggcggcca gtgcgtgccg cgccaggatg gcggggcagc
     4021 gttgtcgaaa actatgcatg gatgtctcct cctgttatgg gtcgttccgc gcggcgtcat
     4081 gtcagcgccc ggtgaacacg ggtttgcgct tttcgcgaaa agcgcgtgcc gcctccaggc
     4141 ggtcttcggt attgatgaga tgggccgaga gatcggtttc atagcgcagg cccgcggcca
     4201 gcggcagctc ggcgccggcg cgcacggcct ccttggcgaa ctgcaccgcc agcggcgggc
     4261 gctcggcgat gcgcgcggca acggcggcgg ccagctcggc gagccccgcc gccggcgcca
     4321 cgcgcgacac caggcccatg gccagcgccc gcgcggcgtc gatgcgctcg ccggtcaata
     4381 ccaggtccaa ggccacgccc tgccccaata ggcgcgtgat gcgctgcgtg ccgccggcgc
     4441 cggggatgat gccgtgcccc gtctcgggaa atccgaacga ggcgtcgtcg gccgcgatgc
     4501 ggatgtcgca cgccagcgcg atctccaggc cgccgcccag gcaatggccg tgcaccgcgg
     4561 cgatcgtcgg cttgaccacg cggtcgaagg cggagatcca atgacgatgg gcgcgctgct
     4621 ggcgccacgc caaggccgaa ccaaccgcgg agaactcctt gatgtcggcc cccacgcaaa
     4681 aggcgcgcgg gcccgcgccg cgcagcacca cgacgcgcac gtcctcgcgc gcatcggccg
     4741 cggccagcgc ggcgggcagc tcggcacgca cggcctcgtt gatggcgttg agcgcctcgg
     4801 gccggttcag cgtcagccag gctacgccgt cgcgcacttc ggtaatcacg gttccggaca
     4861 tcacgcctcc ctggcggcat cgggttcgaa cacgggctcg cgcccctcgc catcggcgcg
     4921 cacccgcacg cgcaggccga tcgcgaccgc ctccgggtcc atgccgacga tgcgggtcat
     4981 caggtgcggc ccctcgtcgg tggccacgat ggccaccaca taaggcacat ccgccgcgta
     5041 agccggagaa ggcgcgcggt acaccaccga atggctgtac acctgccccc gcccgctggc
     5101 gggctgcggc gcgacacgct cgctgccgca cgccgggcag gccggacgcg gataaaaatg
     5161 gaaactgccg cagtcctcgc aacgcggcag cgtgaagccc agttcgcgcg tgcgctgcca
     5221 gtatccggtc ggctcgaggg ccgccgctgc ttgcttgtcg ctcgtactca tgcgtctact
     5281 ccttgcccag gatcacggaa caatgcaggc cgacaatgcc gccgttgccg tgcaccagcg
     5341 cggtcccggc gccggcgacc tgccgctcgc cggcctggcc gcgcaactgg cgcacccctt
     5401 cgagcacatg gaacatgccg ccggccaggc cgggatgccc ggccgacaac aggccgccgt
     5461 gggtcgtgac gggcaagcgt ccgcccaggc cgatggcgcc gttctcgacg aaagccccgc
     5521 cctcgccctt ggcgcaaaag cccaggtcct ccagctcgac ga
//
LOCUS       DASWPO010000352         5717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_533461, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000352 DASWPO010000000
VERSION     DASWPO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533461"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VGC69_18860"
     CDS             complement(<1..350)
                     /locus_tag="VGC69_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY0297395.1"
                     /translation="MSKKKQQKHPKSKHGESGHLCDDACEHRDDPHHHDDPGEPPRHE
                     DVAGHAPRPAHSAPAAGGIYLISPSSAVHNPESLDLACQRLAAEGFKTRVDRAARAVH
                     QRFAGTDKQRLAGLA"
     gene            complement(347..850)
                     /gene="tadA"
                     /locus_tag="VGC69_18865"
     CDS             complement(347..850)
                     /gene="tadA"
                     /locus_tag="VGC69_18865"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY0297396.1"
                     /translation="MAETAVPASPVDELDDRLMALALEQARLAGAAGEVPVGAIVADA
                     DGFVLGAGYNRTITDSDPTAHAEIVALRDAAARLGNYRLPGLSLYVTLEPCVMCMGAM
                     LHARLARVVFGADDPKTGACGSVLDVGAVEKINHHTAIRGGVMAEPCGDILRGFFRER
                     RRKEPTA"
     gene            complement(843..1694)
                     /locus_tag="VGC69_18870"
     CDS             complement(843..1694)
                     /locus_tag="VGC69_18870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0297397.1"
                     /translation="MSDVIALIFDFDDTLAPDSTTGFLESIGVDAAAFWKDSVDPLLS
                     NEDWDPVPAYFYRMIALSREGGHGGPVTRERLQQWGANLPLHDGVATLFSRLRAAVRA
                     AHPKIQLEFYLISSGIGDVVRHTPIAHEFTEIWASEFAYGKDGGIDFPRRLVSFTDKT
                     RYIFHIQKGIIGAEFRGKPFEVNRKVPADRLRVPLDQMVFVGDGYTDIPCFSLIRGKG
                     GYAFGVWDPKHKDKRSRAWGFIEEGRVSNLNQARYGEDAELYQWLEEAVTSLAARITL
                     NARTYRG"
     gene            complement(1706..2422)
                     /locus_tag="VGC69_18875"
     CDS             complement(1706..2422)
                     /locus_tag="VGC69_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297398.1"
                     /translation="MQISFIPLRRAVLALGGALTVALAPAAASAQAPAPLLSSPADIE
                     SHYQAEVARCNAGQSGEDQDTCLREAAAARQQALQGGMGDQGGYSQNAIDRCRRLPAS
                     QQQDCMALMRSSTQTEGSVQSGGVLREMVIPVTPGSASPAPGYAPPPRYAPPAPPVSP
                     AAPASPSVAPVPPSPAFPPPPGSAWATQPSTAMPAGPAGAPAADAPPPSTDSLLIPVP
                     TAPPPPLPAPGTTTAAPIQQ"
     gene            2584..4182
                     /locus_tag="VGC69_18880"
     CDS             2584..4182
                     /locus_tag="VGC69_18880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY0297399.1"
                     /translation="MTSPSSAPNLLDRARLLASITETNWDMVVIGGGATGLGTAVDAA
                     SRGFRTLLVEAADFAKGTSSRATKLVHGGVRYLAQGNISLVREALHERGLLHHNAPHL
                     AWPLGFVVPAYQLIDQPFYGIGLKLYDLLAGKLNLRPSRWLSRDQTLAHAPTLALQQG
                     GRPLRGGILYYDGQFDDARLAVALMRTLYDLGGTALNYVKARGLTRTDGRIDGVELED
                     VLGGTDLGAVKARCVINATGVWVDSVRRMDEPNAAGMVAPSQGVHLTLPRDFLPGDDA
                     ILIPKTDDGRVLFVVPWNGHTLVGTTDAPRADLPMEPKASDADVDFILRTAARYLSRA
                     PTRADVTSVWAGLRPLVKRTEAATKTLSREHTILVSQSGLVTVTGGKWTTYRRMAQDV
                     LDTAIDKHLLPAAACRTASLPLHGAPSDPADLPAPAGTPDSYYGTDLPALRALPGADH
                     VLIAGSGLTEAHVRHAARFELARSAEDVLARRNRALFLDARAALAAAPAVARILAEEL
                     DLGEAWQASTISDFESVARQYLPD"
     gene            4220..4702
                     /locus_tag="VGC69_18885"
     CDS             4220..4702
                     /locus_tag="VGC69_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEY0297400.1"
                     /translation="MSTYDKLQQLLQTNQIDYRLMHHPAAGKSEEVAAIRGTEVSQGA
                     KALVCRVKITSNTRKHVLAVFPADRQADLDAVAAAVGGKKASLVPHELARELTGCEIG
                     AIPPFTFNPELQLVVDPTLRTRHAEIAFNAGRLDASIVMGTEDYFRLAVPAQAPIIKP
                     "
     gene            4719..5489
                     /locus_tag="VGC69_18890"
     CDS             4719..5489
                     /locus_tag="VGC69_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY0297401.1"
                     /translation="MCLAVLALRCIPGVPVLVAANRDEFHARPTAPAARWPGEPAVYA
                     GRDLQAGGTWMGATAGGRYALVTNYREPGRHRPDAPSRGALVEAWLHGGQTAAGYMAQ
                     LHAAGERYNGFNLIVGDAGGAWYYGNRGGAPRELPAGIHGLSNHLLDTPWPKLVRTRR
                     AFERLIGSEPRLPATDALYAMLDDRVPAADDALPDTGLPRDRERLLSSPFIVSPDYGT
                     RVSTVLALHADGRGALHERRFDPQGRIAGDTALAFGGI"
     gene            complement(5486..>5717)
                     /locus_tag="VGC69_18895"
     CDS             complement(5486..>5717)
                     /locus_tag="VGC69_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0297402.1"
                     /translation="RVTKEGVIVIKAQSFRSQEKNREEAVERLLEMVREAARPVRPRR
                     ATRPTLASQRRRVEGKAQRGAIKRLRGRVGDG"
BASE COUNT          828 a         2110 c         1976 g          803 t
ORIGIN      
        1 gccaggccgg ccaggcgctg cttgtcggtg cccgcgaagc gctggtgcac cgcccgggcg
       61 gcgcggtcga cgcgggtctt gaagccttcg gccgccagcc gctggcaggc caggtccagc
      121 gactcggggt tgtgcaccgc cgacgagggc gagatgaggt agatgccgcc ggcggccggc
      181 gcggagtgcg cggggcgggg agcatgcccg gctacgtcct catgacgagg gggctcgcct
      241 gggtcgtcgt ggtgatgcgg gtcgtcgcga tgttcgcacg cgtcgtcgca caggtgcccg
      301 gactcgccgt gcttggactt gggatgtttt tgctgctttt tcttgctcat gcggtaggtt
      361 ccttgcggcg gcgctcacgg aagaacccgc gcaggatgtc cccgcaaggt tcggccatga
      421 cgccgccgcg gatggcggtg tgatgattga ttttctcgac ggcgcccacg tccagcacgc
      481 tgccgcaggc gccggtcttg gggtcgtccg cgccgaacac cacgcgcgcc aggcgcgcgt
      541 gcagcatggc gcccatgcac atcacgcagg gttcgagcgt gacgtagagc gacaggcccg
      601 gcaggcggta attgcccagg cgcgcggccg cgtcgcgcag cgcgacgatt tcggcatggg
      661 cggtggggtc gctgtcggtg atggtgcggt tgtagccggc gcccagcacg aacccatcgg
      721 catcggccac gatggcgccc accggcactt cgccggccgc gccggccagg cgggcctgct
      781 ccagcgccag cgccatcagg cggtcgtcga gttcgtcgac cggcgaagcg ggaacggcgg
      841 tctcagccac ggtaggtccg cgcgttgagg gtgatgcgtg cggccaagct ggtgacagcc
      901 tcttccagcc actggtacag ctcggcatcc tcgccgtagc gcgcctggtt gaggttggac
      961 acccgtcctt cctcgatgaa gccccaggcc cggctgcgct tgtccttgtg cttcggatcc
     1021 cacacgccga aggcatagcc gcccttgccc ctgatcaagg aaaagcacgg gatgtcggtg
     1081 tagccgtcgc cgacgaacac catctggtcg agcggcacgc gcaggcggtc ggccggcacc
     1141 ttgcggttga cctcgaaagg cttgccgcgg aactcggcgc cgatgatgcc cttctggatg
     1201 tggaagatgt agcgcgtctt gtcggtgaag ctgaccaggc ggcgcgggaa atcgatgccg
     1261 ccgtccttgc cgtaggcgaa ttcggaagcc catatctcgg tgaactcgtg cgcgatgggc
     1321 gtatgccgca ccacgtcgcc gatgccgctg gagatcaggt agaactcgag ctggatcttg
     1381 ggatgcgcgg cccgcacggc cgcgcgcagg cgcgagaaca gcgtggccac gccgtcgtgc
     1441 aggggcaggt tcgcgcccca ctgctgcagc cgctcccgcg tgaccgggcc gccgtgcccg
     1501 ccctcgcgcg acagcgcgat catgcgatag aagtaggccg gcaccggatc ccagtcttcg
     1561 ttggacagca gcgggtcgac cgagtctttc cagaaggccg ccgcatccac gccgatgctc
     1621 tccagaaagc ccgtggtgct gtcgggagcc agcgtgtcgt cgaaatcgaa gatcagggca
     1681 atgacgtcgg acatggcggt acgcgtcact gctggatcgg cgcggcggtg gtcgtgcccg
     1741 gagccggcag gggcggcggc ggcgcggtcg gcacggggat gagcagggaa tcggtcgagg
     1801 ggggcggcgc atcggccgcc ggcgcgccgg ccggacccgc cggcatggcc gtggacggct
     1861 gcgtcgccca ggccgagccg ggcggcggcg ggaatgccgg actgggcggg acaggcgcca
     1921 cgctgggcga ggccggcgcc gcaggcgaaa ccggcggcgc gggcggcgca tagcggggag
     1981 gcggcgcata gcccggcgcg ggagacgcgg agccgggcgt gacgggaatc accatttcac
     2041 gcagcacgcc gccgctttgc acgctgcctt cggtctgcgt ggaggatcgc atcaaggcca
     2101 tgcagtcctg ctgctgcgaa gcgggcaggc gccggcagcg gtcgatcgcg ttctggctgt
     2161 agccgccctg gtcgcccata ccgccctgca gggcttgctg gcgcgcggcg gcggcttcgc
     2221 gcaggcaggt gtcctggtct tcgccgctct ggcccgcatt gcagcgcgcc acctcggcct
     2281 ggtagtggga ttcgatgtcg gccgggctgg acagcagcgg cgccggggcc tgggccgatg
     2341 cggcggcggg cgccaaggcg accgtcaagg cgcctcccag ggccaggacc gcacggcgca
     2401 atggaatgaa agaaatctgc atggcatgct ccgtgaaaca cgggcgtcgc gcaagcggcg
     2461 ccacaccaga cagattgtga cacgactgaa cgacaaagcc ggtacgatac attgcagagt
     2521 attgcgccgc gccgcttccg ggcgcggccc ggccagccat cgcccaagga tccacgctgc
     2581 atcatgacga gcccctcctc cgccccgaac ctgctcgacc gcgcccgcct gctggcctcg
     2641 atcacggaaa cgaactggga catggtggtc atcggcggcg gcgccaccgg cctgggcacg
     2701 gcggtcgatg ccgcttcgcg cggattccgc acgctgctgg tggaggcggc cgacttcgcc
     2761 aagggcacgt ccagccgcgc caccaagctg gtgcacggcg gcgtgcgcta cctggcccag
     2821 ggcaatatct cgctggtgcg cgaggccctg cacgagcgcg gcctgctgca ccacaacgcg
     2881 ccgcacctgg cctggccgct gggcttcgtg gtgccggcct accaactcat cgaccagccc
     2941 ttctacggca tcggcctcaa gctctacgac ctgctggccg gcaagctcaa cctgcggccc
     3001 agccgctggc tgtcgcgcga ccagaccctg gcgcacgccc ccacgctggc cctgcagcag
     3061 ggcgggcgcc cgctgcgcgg cggcatcctc tattacgacg gccagttcga cgacgcgcgg
     3121 ctggccgtgg cgctgatgcg cacgctctac gacctgggcg gcacggcgct gaactacgtc
     3181 aaggcgcgcg gcctcacccg caccgacggc cgcatcgacg gcgtcgagct cgaggatgtg
     3241 ctgggcggca cggacctggg ggcagtcaag gcgcgctgcg tgatcaacgc cacgggcgtc
     3301 tgggtcgact cggtgcgccg catggacgaa cccaacgccg ccggcatggt ggcgcccagc
     3361 cagggcgtgc acctgacgct gccgcgcgac ttcctgccgg gcgacgacgc catcctcatc
     3421 cccaagaccg acgacggccg cgtgctgttc gtggtgccct ggaacggcca caccctcgtg
     3481 ggcaccaccg acgcgccgcg cgccgacctg cccatggaac ccaaggcctc cgatgccgac
     3541 gtggacttca tcctgcgcac ggccgcccgg tatctcagcc gcgcgcccac ccgcgccgac
     3601 gtcaccagcg tctgggccgg cctgcgcccc ctggtcaaac gcaccgaggc cgccaccaag
     3661 accctgtcgc gcgagcacac catcctggtg tcgcagtccg gcctggtgac ggtcacgggc
     3721 ggcaaatgga ccacctatcg ccgcatggcg caggacgtgc tcgacaccgc catcgacaag
     3781 catctgctgc cggccgccgc ctgccgcacc gcatcgctgc cgctgcacgg cgcgccgtcc
     3841 gaccccgccg acctgcccgc gccggctggc acgccggaca gctattacgg caccgacctg
     3901 cccgccctgc gcgccctgcc cggcgccgac catgtgctga tcgccggcag cggcctgacc
     3961 gaagcccatg tgcgccacgc ggcccgcttc gagctggccc gcagcgccga ggacgtgctg
     4021 gcgcgccgca accgggccct gttcctcgat gcccgcgccg ccctggccgc ggcgcccgcg
     4081 gtggcccgca tcctggccga ggaactggac ctgggcgagg cctggcaggc gagtaccata
     4141 agcgacttcg agtccgtcgc gcggcaatac ctgccggact gatcctgcca gacccataca
     4201 gccccgcctt cgatacgcca tgagcaccta cgacaagctg cagcagctgc tgcaaaccaa
     4261 ccagatcgac taccggctga tgcaccaccc cgccgccggc aagtccgagg aagtggcggc
     4321 catccgcggc accgaagtca gccagggcgc caaggcgctg gtgtgccggg tgaagatcac
     4381 ctccaacacg cgcaagcatg tgctggccgt atttccggcc gaccgccagg ccgacctcga
     4441 cgccgtggcc gcggccgtgg gcggcaagaa ggcctcgctg gtgccgcatg aactcgcgcg
     4501 cgaactcacc ggctgcgaga tcggcgccat cccgcccttc accttcaacc cggaactgca
     4561 gctggtggtc gaccccacgc tgcgcacccg ccacgccgag atcgctttca atgccggccg
     4621 gctcgatgcc tccatcgtga tgggcaccga agactacttc cgcctggccg tgcccgcgca
     4681 agcccccatc atcaaaccct gatcccccgg gccgccccat gtgccttgcc gtccttgccc
     4741 tgcgttgcat acccggcgtg ccggtgctgg tcgccgccaa ccgcgacgag ttccatgcgc
     4801 ggcccaccgc gcccgccgcg cgctggccgg gcgagcccgc ggtgtatgcc ggacgcgacc
     4861 tgcaggccgg cggcacctgg atgggcgcca ccgccggcgg ccgctacgcc ctggtgacca
     4921 actaccgcga acccggccgc caccggcccg acgccccctc gcgcggcgcg ctggtcgagg
     4981 catggctgca cggcggccag acggcggccg gctacatggc gcagctgcac gcggcgggcg
     5041 aacgctacaa cggcttcaac ctgatcgtgg gcgacgccgg cggcgcctgg tactacggca
     5101 accggggcgg cgcgccgcgc gaactgcccg ccggcatcca cggcctgtcc aaccacctgc
     5161 tggacacgcc ctggcccaag ctggtccgca cccgccgggc cttcgaacgc ctgatcggtt
     5221 ccgagccccg gctgcccgcc acggacgcgc tgtatgccat gctcgacgac cgcgtccccg
     5281 cggccgacga cgccctgccc gacaccggcc tgccgcgcga ccgcgaacgc ctgctgagca
     5341 gccctttcat cgtcagcccc gactacggca cgcgggtctc gaccgtgctg gccttgcatg
     5401 ccgatggccg cggcgcgctg catgaacggc gcttcgaccc gcagggccgc atcgccggcg
     5461 atacggcgct ggcattcggc gggatttagc cgtcccccac ccgcccgcgc agccgcttga
     5521 tcgcgccgcg ctgcgccttg ccctcgacgc gccgccgctg cgaagccagc gtgggccggg
     5581 tggcgcggcg cgggcgcacg ggccgcgccg cctcgcgcac catctccagc agccgctcga
     5641 ccgcctcttc gcggtttttt tcctggctgc gaaacgactg cgccttgatg acgatgacgc
     5701 cttccttggt gacgcgc
//
LOCUS       DASWPO010000353         7501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2142128, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000353 DASWPO010000000
VERSION     DASWPO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142128"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VGC69_18900"
     CDS             <1..647
                     /locus_tag="VGC69_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297403.1"
                     /translation="LDDEERAKPSHSHKLWAAICQPAVLYLCLIYALIQASVYGVVFY
                     LPTQVAGLMGAKVGFTVGLVSAIPWLCALLATWWIPSHADRTGHRRNTASLTLVMAAA
                     GIAVSVSVSTPLAGVLALCFAAAGFIAVQPVFWTFPSNALSGSVAAGGIALINSLGGV
                     GGFVAPIVKNWAETVFHSPAAGLYLLAGTTLLAAVLVLGLRDTAPAAARTHAAA"
     gene            710..1894
                     /gene="rhmD"
                     /locus_tag="VGC69_18905"
     CDS             710..1894
                     /gene="rhmD"
                     /locus_tag="VGC69_18905"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HEY0297404.1"
                     /translation="MALPTIKQVRAYTLKGGGADYHDQQDGHWIDDHIATPMSKYPAY
                     RQSRRSFGINVLGTLVVQIEASDGSVGFAVTTGGEPAAYIVEKHLARFLEGARVTDIE
                     LIWDQMYQSTLYYGRKGLVINTLSGVDLALWDLLGQVRGEPVHQLLGGAVRDELQFYA
                     TGARPDLAQKMGFIGGKLPLHHGPAEGEEGLRKNIEELATMRERVGPDFWLMLDCWMS
                     LDLNYATRLAQAAREYGLKWIEEALPPDDYWGYASLRKNVPAGMLVTTGEHEASRWGF
                     RMLLEMGCCDIIQPDVGWCGGITELLKISALADAHQALVIPHGSSVYSYHFVATRQNS
                     PFAEFLMMAPKADQVVPMFHPQLLGEPVPANGRMRLSELDKPGFGVTLNPDCVLHRPY
                     TH"
     gene            1914..2714
                     /locus_tag="VGC69_18910"
     CDS             1914..2714
                     /locus_tag="VGC69_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEY0297405.1"
                     /translation="MQLPINPFKAALAQGTPQYGIWAGFATPYAAEIVAGTGYDWMLV
                     DGEHAPNTAPSLLGQLQAVAPYATHPVVRPVNGDPALIKQLLDLGAQTLMVPMVETAE
                     QARALVRAMRYPPGGIRGVGGGLTRATRWDAVADYIRTAHEQLCLIVQVESRLGVKNV
                     AEIAAVEGVDAVFIGPADLSTGLGHAGDASQPEVQASIRRAIDATRAAGRACGILAPR
                     EEDARRYAEWGCTFIAVGIDISLLRQAAIDNLARYRGPGDAPAPSRTY"
     gene            2749..4182
                     /gene="aldA"
                     /locus_tag="VGC69_18915"
     CDS             2749..4182
                     /gene="aldA"
                     /locus_tag="VGC69_18915"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY0297406.1"
                     /translation="MTTVPMLQNYIGGEFSSSAELHDVLNPATGERLAQAPMASQQEV
                     DRAIAAARGAQKAWARKTAIERAGHLRRIAAKLREHAPRLARAITQEQGKILGLAEVE
                     VHFTADYMDYMAEWARRLEGEIITSDRPDESIFLLRKPLGVVAGILPWNFPFFLIARK
                     MAPALLTGNTIVIKPSEETPINCHEFTQLLAQTDLPAGVFNVVYGQGAVGGALSAHPG
                     VDMISFTGSVPTGSRIMAAAAGNITKLNLELGGKAPAIVLADADLDLAVKAIRDSRII
                     NTGQVCNCAERVYVERKVADEFVARIAQAMAATRYGDPIAQPDVEMGPLINQKGLDGV
                     AAKVRTAIGAGAQLVTGGRVADLGRGFHYEPTVLAGCRADMEIMRKEVFGPVLPVQVV
                     DDLDEAIALANDSEYGLTSSVYTRDLAKAMHAMREIDFGETYINRENFEAMQGFHAGV
                     RKSGIGGADGKHGLYEYTHTQVVYVQS"
     gene            complement(4228..4809)
                     /locus_tag="VGC69_18920"
     CDS             complement(4228..4809)
                     /locus_tag="VGC69_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY0297407.1"
                     /translation="MTTLDPALQALQSRQSLRFVRGPGPSDEELAQILQAGMSAPDHG
                     ALRPWRYVIIRGEAVGKLADMALDAVKRTGDPRMTPDKEKNVRAWLADVPLVLGVAQK
                     SFFNGKIPEHEQMLAVGASVMNVLNAAYMLGYGAFWSTGLGTHVPEVQEALGFDPLEY
                     RFLGFLAIGTPAVELPKAQRSDYREFVTEWSGA"
     gene            complement(4890..5195)
                     /locus_tag="VGC69_18925"
     CDS             complement(4890..5195)
                     /locus_tag="VGC69_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HEY0297408.1"
                     /translation="MATPNVPPRIVIEYCTLCQWLLRAGWMAQELLSTFSTDLGEVAL
                     VPGTGGVFRIRYDDQVIWDRTVDGGFPDARALKQRVRDRLDPDRDLGHIDGHGKAAG"
     gene            complement(5321..6409)
                     /locus_tag="VGC69_18930"
     CDS             complement(5321..6409)
                     /locus_tag="VGC69_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0297409.1"
                     /translation="MTDDKTSHGGRPEDSPALHQLYADFKSNHLNPLWTQIGDLMPRH
                     PKPKAVPHVWKWSTLYPLARQSGELVPVGRGGERRAIGLANPGLGGEAYISPTLWCAI
                     QYLGPHETAPEHRHSQNAFRFVVEGEGVWTVVNGDPVHMSRGDFLLTPGWCFHGHQNV
                     RDTPMAWLDGLDIPFSYTNDVGFFEVGKNGLTDSSTPDVSRSERLWCHPGLRPLSGLQ
                     NTVSSPIGAYRWEHTDRALAEQLKLEVEGYPGVAEPGHAAIRYVNPTTGGDVMPTIRA
                     EFHRLRAGVGTAPKREVGSSVFQVFEGRGAVILGDATHKLEKGDIFVVPSWVPWSLQA
                     ESQFDLFRFSDAPIIERLHFDRPAVAGL"
     gene            complement(6999..7361)
                     /locus_tag="VGC69_18935"
                     /pseudo
     CDS             complement(6999..7361)
                     /locus_tag="VGC69_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
BASE COUNT         1319 a         2546 c         2441 g         1195 t
ORIGIN      
        1 tcctcgacga cgaagaacgc gccaagccct cgcacagcca caagctatgg gccgcgatct
       61 gccagcccgc ggtgctgtac ctgtgcctga tctacgcgct catccaagcc agcgtctacg
      121 gcgtggtgtt ctacctgccc acccaggtgg cggggctgat gggcgccaag gtcggcttta
      181 ccgtcggcct ggtttcggcg attccgtggc tgtgcgccct gctggccacc tggtggatac
      241 ccagccacgc cgaccgcacg ggccatcgcc gcaacacggc cagcctgacg ctagtcatgg
      301 cggcggccgg catcgccgtc tcggtcagcg tatccactcc cctggccggc gtcctggcgc
      361 tgtgcttcgc ggccgcgggc ttcatcgccg tccagccggt cttctggacc ttcccgtcga
      421 acgccctgtc gggcagcgtc gccgcgggcg gcatcgcgct catcaactcg ctgggcgggg
      481 tcggcggctt cgtggcgccc atcgtcaaga actgggccga gacggttttc cactccccgg
      541 ccgcgggcct gtacttgctg gccgggacaa ccttgctggc cgccgtgctg gtgctaggcc
      601 tgcgcgacac cgcgccggcc gcggcgcgca cgcatgccgc ggcctgagcc atcccacccc
      661 ccggcacgac acccattaca cacagccatc acgaacgcag gagttttcca tggcactccc
      721 caccatcaag caggtccgcg cctataccct gaaaggcggc ggcgcggact accatgacca
      781 gcaggacggg cactggatcg acgatcacat cgccacgccc atgagcaagt accccgccta
      841 ccggcaaagc cgccgcagct tcggcatcaa cgtgctcggg acgctggtgg tgcagatcga
      901 ggcgtccgac gggtcggtgg gcttcgcggt gacgaccggc ggcgagcccg cggcctatat
      961 cgtggaaaag cacctggcgc gcttcctcga gggcgcccgc gtcaccgaca tcgagctcat
     1021 ctgggaccag atgtaccagt ccacgctcta ctacggccgc aagggcctgg tcatcaacac
     1081 cctctccggc gtcgacctgg cgctgtggga cctgctgggc caggtgcgcg gcgagcccgt
     1141 gcaccagttg ctgggcggcg cggtgcgcga cgagttgcag ttctacgcca ccggcgcgcg
     1201 gcccgacctg gcccagaaga tgggtttcat cggcggcaag ctgcccttgc accacggccc
     1261 ggccgaaggc gaggaagggc tgcgcaagaa catcgaagaa ctggccacca tgcgcgagcg
     1321 cgtcggcccg gacttctggc tgatgctcga ctgctggatg agcctggacc tgaactacgc
     1381 cacgcgcctg gcgcaggccg cgcgcgaata cgggctcaag tggatcgagg aggccctgcc
     1441 gcccgatgat tactggggct atgcctcgct gcgcaagaac gtgcccgccg gcatgctggt
     1501 cacgacgggc gagcacgagg cctcgcgctg ggggttccgc atgctgctcg agatggggtg
     1561 ctgcgacatt atccagcccg acgtgggctg gtgcggcggc atcaccgaac tgctgaagat
     1621 ctccgcgctg gccgacgcgc accaggcgct ggtgatcccg cacggctcgt cggtctatag
     1681 ctaccacttc gtcgccacgc gccagaacag ccccttcgcg gagttcctga tgatggcgcc
     1741 caaggccgac caggtcgtgc cgatgttcca cccgcagctg ctgggcgagc ccgtgccggc
     1801 caacggccgc atgcgcctgt ccgagctgga caagccgggc ttcggcgtga cgctcaaccc
     1861 cgactgcgtg ctgcatcggc cgtacacgca ctaaaacacc aaggaatacg cacatgcaat
     1921 tgcccatcaa ccccttcaag gcagccctgg cccagggcac gccgcaatac ggcatctggg
     1981 ccgggttcgc cacgccctac gcggccgaga tcgtggccgg caccggctac gactggatgc
     2041 tggtcgacgg cgaacacgcg cccaacacgg cgcccagcct gctgggccag ctgcaggccg
     2101 tggcgcccta cgccacgcat cccgtcgtgc gccccgtcaa cggcgacccc gcgctcatca
     2161 agcagttgct ggacctgggc gcgcagacct tgatggtccc catggtcgag acggccgagc
     2221 aggcgcgcgc gctggtgcgc gccatgcgct acccgccggg cggcatccgc ggcgtaggcg
     2281 ggggcctgac ccgcgccacg cgctgggacg ccgtcgccga ctacatccgg accgcgcacg
     2341 agcagctatg cctgatcgtg caggtggaat cgcgcctggg cgtgaagaac gtcgccgaga
     2401 tcgccgcggt ggaaggcgtg gacgccgtct tcatcggccc ggccgatctg tccaccggcc
     2461 tgggccatgc cggcgacgcc agccagcccg aggtgcaggc cagcatccgc cgcgccatcg
     2521 acgccacgcg cgccgccggc cgcgcctgcg gcatcctggc gccgcgcgag gaagacgcgc
     2581 gccgctacgc cgaatggggc tgcacgttca tcgcggtggg catcgacatc agcctgctgc
     2641 gccaagccgc catcgacaac ctggcgcgct atcgcggccc cggcgacgcc cccgcgccgt
     2701 cacgcacgta ctgagcgcgc gccccacccc acccagagga gactccccat gactactgtg
     2761 cccatgctcc agaactacat cggcggcgaa ttttccagca gcgccgaact gcacgacgtc
     2821 ctcaaccccg ccacgggaga acgcctggcc caggccccca tggcgtcgca gcaggaggtc
     2881 gaccgcgcca tcgccgccgc gcgcggcgcg cagaaagcct gggcgcgcaa gaccgccatc
     2941 gagcgcgcgg gacacctgcg ccgcatcgcc gccaagctgc gcgagcacgc gccgcgcctg
     3001 gcgcgcgcca tcacgcagga gcaaggcaag atcctgggcc tggccgaagt cgaggtgcac
     3061 ttcaccgccg actacatgga ctacatggcc gaatgggcgc gccgcctgga aggcgaaatc
     3121 atcaccagcg accgccccga cgaaagcatc ttcctgctgc gcaagccgct gggcgtggtg
     3181 gcgggcatcc tgccgtggaa cttcccgttc ttcctgatcg cgcgcaagat ggccccggcg
     3241 ctgctcacgg gcaacaccat cgtcatcaag cccagcgaag aaacgcccat caactgccac
     3301 gaattcaccc aactgctggc gcagaccgac ctgccggcgg gcgtgttcaa cgtggtgtac
     3361 ggccaaggcg ccgtcggcgg cgccttgtcg gcccacccgg gcgtggacat gatcagcttc
     3421 accggcagcg tgcccacggg ctcgcgcatc atggccgccg cggccggcaa catcaccaag
     3481 ctcaacctgg agctgggcgg caaggcgccc gccatcgtgc tggccgacgc cgacctcgac
     3541 ctggcggtga aggccatccg cgactcgcgc atcatcaata ccggccaggt ctgcaactgc
     3601 gccgagcgcg tctatgtcga gcgcaaggtg gccgatgaat tcgtcgcgcg catcgcccag
     3661 gccatggcgg ccacgcgcta tggcgatccc atcgcccagc ccgacgtcga gatgggcccg
     3721 ctcatcaacc agaagggcct ggacggcgtc gccgccaagg tgcgcacggc catcggcgcc
     3781 ggcgcccagc tcgtcaccgg cggccgggtc gccgacctgg gccgcggctt tcactacgag
     3841 cccacggtgc tggccggctg ccgcgccgac atggagatca tgcgcaagga agtcttcggt
     3901 ccggtgctgc ccgtccaggt cgtcgacgac ctggacgagg ccatcgccct ggccaacgat
     3961 tccgaatatg gcctgacctc gtcggtgtac acgcgcgacc tcgccaaggc catgcacgcc
     4021 atgcgcgaga tcgacttcgg cgaaacctat atcaaccgcg agaacttcga ggccatgcag
     4081 ggctttcatg ccggcgtgcg caaatccggc atcggcggag cggacggcaa gcacggtctc
     4141 tacgagtaca cgcacaccca ggtggtctac gtgcaaagct aggcggacaa ggaagaggcc
     4201 tgaagaaagc caagcccccg ccggccatca agcccccgac cactccgtga cgaactcacg
     4261 gtagtcggag cgctgcgctt tgggcagttc aacggcgggc gtgccgatgg ccaggaaccc
     4321 caggaagcgg tattccagcg ggtcgaagcc cagcgcttcc tggacctcgg gcacatgggt
     4381 gcccaggccg gtgctccaga acgcgccgta gcccagcatg taggccgcgt tcagcacatt
     4441 catcaccgac gcgccgacgg ccagcatctg ctcgtgctcg gggatcttgc cgttgaagaa
     4501 gcttttttgc gccacgccca gcaccagggg cacgtcggcc agccaggcgc gcacgttctt
     4561 ttccttgtcg ggcgtcatgc gcgggtcgcc ggtgcgcttg acggcgtcga gcgccatgtc
     4621 ggccagcttg ccgaccgcct cgccgcggat gatcacgtag cgccatggac gcagggcgcc
     4681 gtggtcgggc gcgctcatgc cggcctgcag gatctgcgcc agctcttcgt cggaaggccc
     4741 cgggccgcgc acgaaccgga gggactggcg ggactgcagg gcttgtaggg cgggatcgag
     4801 ggtagtcatt ctgtggagtt acgcaaaggg actgcgaaga tgagaaagat tctcatcata
     4861 cgcctattgg cccgatactc aggcatggtt cagcccgcgg ccttcccatg cccgtcgata
     4921 tgccccaggt cgcggtccgg atcgagccgg tcgcgcaccc gctgcttgag cgccctggca
     4981 tcgggaaagc cgccgtcgac ggtacggtcc cagatcacct ggtcgtcgta gcggatgcgg
     5041 aacaccccgc ccgtgcccgg caccagggcc acctcgccca agtcggtgga aaaggtggag
     5101 agcagttcct gggccatcca gccggcgcgc agcagccatt ggcagagcgt gcagtattcg
     5161 atgacgatgc gaggcggaac gttcggagtc gccatgccgt gctcctgata cgaaacggtt
     5221 gtcaggcctt attgtgcctt gcggccggct caagagcgac tggaaacgaa aaagaccgcg
     5281 caggcggcct tttccatcga agccccgaaa ggaattaggc ctacagcccg gcgaccgcag
     5341 gccggtcgaa atgcaggcgc tcgatgatgg gcgcgtcgga gaagcggaac aggtcgaact
     5401 ggctttcggc ctgcagcgac cagggcaccc agctcggcac gacgaagatg tcgcccttct
     5461 cgagcttgtg ggtcgcgtcc cccaggatga cggcgccgcg tccttcgaac acctggaaga
     5521 ccgaggaacc gacctcgcgc ttgggcgcgg tgccgacgcc tgcgcgcagc cggtggaatt
     5581 cggcgcggat cgtcggcatg acgtcgccgc ccgtcgtcgg attgacgtag cggatcgccg
     5641 cgtggcccgg ctcggcgacg ccgggatagc cttcgacctc gagcttcagc tgctcggcca
     5701 gcgcgcggtc ggtgtgttcc cagcgatagg cgccgatggg cgaagacacg gtgttctgca
     5761 ggccggacaa gggccgcagg ccggggtggc accacaagcg ctcgctgcgc gaaacatccg
     5821 gcgtgctgct gtcggtcagg ccattcttgc ccacctcgaa gaacccgacg tcgttcgtgt
     5881 agctgaacgg gatgtccagg ccgtcgagcc aggccatcgg cgtgtcgcgc acattctggt
     5941 ggccgtggaa gcaccagccg ggggtgagca ggaaatcgcc gcggctcatg tggaccgggt
     6001 cgccgttgac gaccgtccag accccttcgc cctcgaccac gaagcggaag gcgttctgcg
     6061 aatggcgatg ctcgggcgcg gtctcgtgcg ggccgagata ctggatcgcg caccacaggg
     6121 tgggcgaaat ataggcctcg ccgcccaggc ccggattggc gagcccgatg gcgcggcgct
     6181 ccccgccgcg gccgaccggg acgagttcgc ccgattgccg ggcgagcgga tacagcgtcg
     6241 accatttcca gacgtgcggc acggccttgg gcttgggatg ccgaggcatc aggtcgccga
     6301 tctgggtcca gagaggattg aggtggttgg acttgaagtc ggcgtagagc tgatgcagcg
     6361 cggggctgtc ttccggccgg ccgccatggg atgtcttatc gtccgtcatt gtcttgttcc
     6421 tcctattggt atggtctggg gactaagcca ttttatcttc ccgggtatac gataaattat
     6481 tcatttttcg tatcatccat actaaaaaaa gaatagacca gcttcaccta tctcttgtgc
     6541 aaatcggcga tgagggacgc ggaggcggct cagcggggga tgtaggtttt gatcacctct
     6601 gcccaaaata gacagcctgt cgccgtgatc agaacgaagc caagcaatag tgcattgaca
     6661 ggccgcttgc gccccacttt gttcaagatg atcgggaagg tagccccaag caagacgagg
     6721 cctgccgcgg taatgaggga aggcatgaca ttatttgaca actgacacta tcagtagcgg
     6781 aatgtatcac gcagcagcca ttctcattgg tgaggggtgt aaatggggac aattcaatat
     6841 gtatcaaaag atccgccgtc atgcgccacc cattttggcg tgtggcgggt cgtggcgcaa
     6901 gccaagcaaa aacagcccca cttgggggct gtcagcttga tatggcaagg atgggcaagc
     6961 agcggatcga agacgctact ttcgaagcgg ctggctgttc actgcagcgt gcgcgtgaac
     7021 acgaacttgc cgtctcgcca atccaccggc accacgtcgc gcgggccgaa gacgccttcc
     7081 agtatcagct tggcgaccgg attctcgatc ttctgctgaa tggcgcgctt caaggggcgc
     7141 gcgccaaaca ccgggtcgaa gcccgtgcgg gccagctcgg ccagcgcggc atcgctgatc
     7201 tccaggcgca tctcctgctt gtccagccgg tcggaaaggc gcttgagctg gatgcgggcg
     7261 atggactcga tgtgctgcgc ttccagtccg tggaacacca cgacttcgtc gatgcggttg
     7321 aggaactcgg gccgcagcgt ctgcttgagc tcgtcccaca aatgcgtatc ggctaagatg
     7381 atgtacttgg tgcggcacgg actgggtacg gtgtaagcgc aggaccagca gcggttctca
     7441 agcatctgtt ccctctaaat ccccattgag cagaagcgtt gtcgagcgcc gttaatgtat
     7501 c
//
LOCUS       DASWPO010000354         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1873090, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000354 DASWPO010000000
VERSION     DASWPO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873090"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VGC69_18940"
     CDS             complement(<1..245)
                     /locus_tag="VGC69_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY0297410.1"
                     /translation="MSQTAQSPALAEARLRTSMPVLLGWAIVIALLGWSWKGADMRPW
                     DFVANGSNMATFIADFFPPNFREWRMYIDETIVTIQIA"
     gene            complement(319..1305)
                     /gene="phnD"
                     /locus_tag="VGC69_18945"
     CDS             complement(319..1305)
                     /gene="phnD"
                     /locus_tag="VGC69_18945"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0297411.1"
                     /translation="MFVKTLSALFVTAAVVSAPAAHAAGDVLNFGLISTDTSTALRDR
                     WQPVLDDMSKATGLTIKPFFASDYAGVIEGMRFNKVQVAWFGNAGAVQAVDRSNGEVF
                     ARTTYANGDPGYWSVIVTAKDSPVKSIDEVIKNPKAYSYGAGDPNSTSGYLVPNFYLW
                     IANKINPSQDFKASRSANHEANLMAAMNKQVDVAVTNTETLQLYNDSNKDKGKKWEDS
                     VRVLWKSPIIPTDPIVRRKDLPDDVKKKISDFLLNYGKTPEEQAKLAKLSFGKFEASS
                     DKQLLVIRQLGLAGDKAKLESDTTTPDAEKKTKIAEIDAKLADLDKEIKANQ"
     gene            complement(1347..2207)
                     /gene="phnC"
                     /locus_tag="VGC69_18950"
     CDS             complement(1347..2207)
                     /gene="phnC"
                     /locus_tag="VGC69_18950"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEY0297412.1"
                     /translation="MTYAIDVQGLTKSFKAGRKALDNVSLQVHPGEMIALLGASGSGK
                     STLLRHLAGFIAGDAGCGSIRVNGHVIQENGRVNRSIRRSRAGIGFVFQQFNLVGRLP
                     VIVNVLTGLLTRMPMWRSFFRLFTRAEVAQGLQALRHVGIHETAFQRASTLSGGQQQR
                     AAIARTLVQNARIVLADEPIASLDPESSRRVMDTLAQINVDRQVTVVVSLHQVDTALR
                     YCQRVVALRQGQVVYDGPSCDLTHSMLQDLYGAELDQLREPAVALPPANDEAPFDAAA
                     PKVLDAAAAS"
     gene            2391..>3171
                     /locus_tag="VGC69_18955"
     CDS             2391..>3171
                     /locus_tag="VGC69_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0297413.1"
                     /translation="MRGWLMGLVLIGFFGSWAWPAGAQERVRFPSLDAQATPLDGYLY
                     AAPGQGRHPALVFMHGCGGLLTKSGKVVSRERQWAQRFNDLGVTVLMVDSMTPRQHGE
                     MCAPAHFDASIYRARPFDAYGALHFLRSRDDIRADRVGLVGWSAGGGAVLNAIRASSP
                     ARRAAGQGESFRAAVAFYPASCSRKRQGGDWSSPVPLLVLLGGKDVWIPLDDCKDLLG
                     HASPAAQLAWHVYDDAYHDFDWPGLRTHEAPAFRTRAGVVPI"
BASE COUNT          485 a          927 c         1162 g          597 t
ORIGIN      
        1 gcgatctgga tggtgacgat ggtttcgtcg atatacatcc gccactcccg gaaattggga
       61 gggaagaagt cggcgatgaa cgtcgccata ttgctgccgt tggcgacaaa atcccagggg
      121 cgcatatcgg cgcctttcca cgaccagccc agcagcgcaa tgacgatggc ccagcccagc
      181 aggacgggca tggacgtgcg caggcgcgct tcggcgaggg cgggagattg tgcggtttgg
      241 ctcatggcgg cgtggcgagg atatgaaaag caaaaacgcc gcggccgcct gggcgggccc
      301 gcggcgctgc aaggccgatt actggttggc cttgatttcc ttgtcgagat cggccagctt
      361 ggcatcgatc tcggcgatct tggtcttctt ctcggcgtcg ggggtcgtgg tgtcgctctc
      421 gagcttggcc ttgtcgccgg ccaggccaag ctggcggatc accagcagct gcttgtcgct
      481 ggaggcttcg aacttgccga agctcagctt ggccagcttg gcctgctctt cgggggtctt
      541 gccgtagttg aggaggaagt ccgagatctt cttcttcacg tcgtcgggca gatccttgcg
      601 gcgcacgatg gggtcggtcg ggatgatggg cgacttccac aggacgcgca cgctgtcttc
      661 ccacttcttg cccttgtcct tgttgctgtc gttgtacagc tgcagggtct cggtgttggt
      721 gacggcgacg tcgacctgct tgttcatggc ggccatcaga ttggcttcgt ggttggccga
      781 gcggctggcc ttgaagtcct gggaggggtt gatcttgttg gcgatccaca ggtagaagtt
      841 ggggaccaga tagcccgagg tcgagttcgg gtcgccggcg ccgtagctgt aggccttggg
      901 gttcttgatg acttcgtcga tgctcttgac cgggctgtcc ttggcggtaa cgatcaccga
      961 ccagtagccc gggtcgccgt tggcgtaggt ggtgcgcgcg aacacttcgc cattggaccg
     1021 gtccacggcc tggacggcgc ccgcgttgcc gaaccaggcg acctgcacct tgttgaagcg
     1081 catgccttcg atcacgccgg cgtagtccga ggcgaagaag ggcttgatgg tcaggccggt
     1141 ggccttgctc atgtcatcca ggacgggctg ccagcggtca cgcagggcag tggacgtatc
     1201 ggtcgagatg aggccgaagt tcaggacatc gccggcggca tgggcagccg gggcgctgac
     1261 gacagcggcc gtgacgaaca gggcggaaag cgtcttgacg aacatgaatg aaaggctccg
     1321 tgggggcggc tagaaaagca gggtggtcag gatgcggctg ccgcatccag aaccttgggc
     1381 gcggccgcgt cgaaaggcgc ttcgtcgttg gccgggggca gggcgacggc gggctcgcgc
     1441 agctggtcga gctcggcgcc gtacaggtcc tgcagcatgg agtgcgtcag gtcgcaggag
     1501 gggccgtcgt agacgacctg gccctggcgc agggcgacca cgcgctggca gtagcgcagg
     1561 gcggtgtcga cctggtgcag cgacaccacc acggtgacct ggcggtcgac gttgatctgg
     1621 gccagcgtgt ccatcacgcg gcgggaggac tcggggtcga gcgaggcgat gggctcgtcg
     1681 gccaggacga tgcgggcgtt ctgcaccagg gtgcgggcga tcgcggcgcg ctgctgctgg
     1741 ccgccggaca gcgtgctggc gcgctggaag gcggtttcat ggatgccgac gtggcgcagg
     1801 gcttgcaggc cctgggcgac ctcggcgcgc gtgaacaggc gaaagaagct gcgccacatg
     1861 ggcatgcgcg tcagcaggcc ggtgagcacg ttgacgatca ccggcaggcg gccgaccagg
     1921 ttgaactgct ggaagacgaa gccgatgccc gcgcgcgaac ggcggatcga acggttcacg
     1981 cggccgtttt cctggatgac gtggccgttg acgcggatgc tgccgcagcc cgcgtcgccg
     2041 gcgatgaaac cggccaggtg gcgcagcagg gtggatttgc ccgagcccga cgcgcccagc
     2101 agggcgatca tctcgcccgg atggacttgc agcgacacgt tgtcgagcgc tttgcggccg
     2161 gccttgaaac tcttggtcag tccttgtacg tcgatggcgt aggtcatggg ggctccggtg
     2221 caatattgct ggagcgagta tgcggagctt aaatgaccaa gccgtgaaaa cgtattcagt
     2281 ggcggacatg aatgtcagct caattacatg ggtttgtcat ttccttgaca tggttgtatc
     2341 tgggtatagt gcatagcgtg cggattcggc atgaaggcgg gaggagacct atgcgcggct
     2401 ggctgatggg cttggtgctg atcgggtttt ttggatcctg ggcatggccg gccggcgccc
     2461 aggaacgggt gcgttttccg tccctggacg cgcaggccac cccgctcgac ggctatctct
     2521 acgccgcgcc cgggcagggg cggcatcccg ccctggtttt catgcatggc tgcggcggct
     2581 tgctgacaaa gtcaggcaaa gtcgtctcgc gggagcggca gtgggcgcag cgcttcaatg
     2641 acctgggcgt gacggtgctg atggtcgaca gcatgacgcc gcggcagcac ggcgagatgt
     2701 gcgcgccggc gcatttcgat gcctccatct accgggcgcg gcctttcgac gcttacgggg
     2761 ctttgcattt cttgcggtcg cgcgacgaca tccgggccga tcgcgtcggc ctggtcggct
     2821 ggtcggcggg cggcggcgcc gtgctcaatg ccatccgcgc gtccagcccc gcccgccggg
     2881 cggcgggcca gggcgagtcg ttccgggccg ccgtggcttt ctatcccgcc agctgcagcc
     2941 gcaagcggca gggcggcgac tggtccagcc cggtgccgct gctggtgctc ctgggcggca
     3001 aggatgtctg gattccgctg gacgactgca aggacttgct cgggcatgcg tcgccggccg
     3061 cgcagctggc gtggcacgtc tacgacgacg cctaccatga cttcgactgg ccgggcctgc
     3121 gcacgcatga agcgcccgcc ttccgcaccc gggcgggcgt ggtccccatc g
//
LOCUS       DASWPO010000355        14311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_891624, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000355 DASWPO010000000
VERSION     DASWPO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 14311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14311
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891624"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VGC69_18960"
     CDS             <1..1196
                     /locus_tag="VGC69_18960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0297414.1"
                     /translation="VRRAEQAKFDLVFFADTPDTSADLHPGMMVRLEPLVLLGALSMV
                     TSRLGLVATVSTTYSQPYNVARAFASLDRMSGGRIGWNVVTGSSPNAAYNFGNEHHPD
                     HGSRYEMATEYLKVAKGLWDSWEDDALVADKATGVYMDTTKMHTLNHEGKYYQVKGPL
                     NASRPPQGYPVIFQAGASDTGKDFAAATAEVVFATQSILEDAVAFAKDLKDRVEKAGR
                     SRDSLKIMLGVAPMVGDSYEDAQRKVAELARLVKPEVALKTLSDRLGHDMSKFPLDEP
                     LPELPQSTMMQGHAVVLKSIAKKYNMTLRQLLDYAAVSSGHRLLMGTAEQVADDLQLW
                     FEAGAADGFVVLPSFYPTPFDRFVDEVVPILAERGVFRKEYAGSTLREHLGLERPAHP
                     LTLGR"
     gene            1214..1702
                     /locus_tag="VGC69_18965"
     CDS             1214..1702
                     /locus_tag="VGC69_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY0297415.1"
                     /translation="MDKIIAQDEFRDAVSRLGAAVHVVTTNGEGGLAGFTASAVCSLT
                     DTPAMLLVCLQKKSSAYESVKKNGVVCVNMLGSAGLELCKAFSGKTPMADRFADLAWK
                     SAATGSPVLPDSMIAFDCTVTEFKSVETHEILLCQVREIIYGDTQQPALIYYNRAFHH
                     LN"
     gene            complement(1734..2060)
                     /locus_tag="VGC69_18970"
     CDS             complement(1734..2060)
                     /locus_tag="VGC69_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297416.1"
                     /translation="MAAASEIDAPARISRLTSDVIDKDGKPIIDPENNKKTPQQGAAT
                     SVWCATSPRLDGMGGVYCVDCDIAQALPSDESPELHGVRPRAADPVAAGRLWQLSEQL
                     TGVRID"
     gene            1999..2202
                     /locus_tag="VGC69_18975"
     CDS             1999..2202
                     /locus_tag="VGC69_18975"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0297417.1"
                     /translation="MTSLVKREIRAGASISEAAAKVGYASESAFRNAFKRVTDMAPGQ
                     YRQTTERVTAEPRGSPNAETHDF"
     gene            complement(2265..4085)
                     /gene="typA"
                     /locus_tag="VGC69_18980"
     CDS             complement(2265..4085)
                     /gene="typA"
                     /locus_tag="VGC69_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY0297418.1"
                     /translation="MSRALRNVAIIAHVDHGKTTLVDQLLRQSGTFRENQAMVERVMD
                     SNDLEKERGITILAKNCAVEYEGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTIFVTRKALALGLKPIVVVNKIDRPGARPDFVINATFELFDKLGATEEQLDF
                     PVVYASGLSGYAGMTDDVRSGDMRPLFDAILKYVPQRDDDPNGALQMQITSLDYNNYV
                     GKIGVGRITRGRMRPGMDIVFLDGPEGAVQRGRINQVLKFSGLERVQVEEAEAGDIVL
                     INGIEDLNIGTTVTDPLTPEALPMLRVDEPTLTMNFMVNTSPLAGREGKFVTSRQLRD
                     RLDRELKANVALRVRETGDDTVFEVAGRGELHLTILLETMRREGYELAVSRPRVVFQE
                     IDGVKCEPFEALTVDVEDAHQGGVMEELGRRKGDLQDMQPDGRGRTRLEYIIPARGLI
                     GFQNEFLTLTRGTGLMSHIFHEYAPVRDGSIGERRNGVLISQDNGDAVAYALWKLQDR
                     GRMFVSPGEALYEGMIIGIHSRDNDLVVNPIKGKQLTNVRASGTDEAVRLVPPIQMSL
                     EYAVEFIDDDELVELTPKSIRLRKRYLSEHERRKASREGA"
     gene            complement(4100..4927)
                     /gene="truB"
                     /locus_tag="VGC69_18985"
     CDS             complement(4100..4927)
                     /gene="truB"
                     /locus_tag="VGC69_18985"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY0297419.1"
                     /translation="MAKRRGQPLDGVLLLDKPVGLSSNHALQRAKRTVDAAKAGHTGT
                     LDPFATGLLVCCMGRATKISGAMLDADKTYLATLQFGEETDSGDLTGIVTARAAEGFA
                     GVEEQALRDVLSRFMGKIEQIPPMYSALKRDGKPLYEYARQGIELERAPRQITIYRNE
                     LLSGFASTRAMQAVIEVACSKGTYIRTLAQDIGRALGCYAHLAALRRTHAGPFTLERA
                     VTLDTLQAMPDPKLALLALNELPAGLLPPGRSQGESGPPEGQQTEGAAWGLSPLPVK"
     gene            complement(4961..5368)
                     /gene="rbfA"
                     /locus_tag="VGC69_18990"
     CDS             complement(4961..5368)
                     /gene="rbfA"
                     /locus_tag="VGC69_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY0297420.1"
                     /translation="MSRHKSKSIPGRNLRLADQIQKDLAEIVQREVDVSRSGLITISG
                     VDLSADYAHAKVYFTVLGAEPDATAALLNEKSGWLHSLLYKLLHIHTVPTLRFYHDPQ
                     TERAIEMSSLIDRATRDNPGAASGVPDEPEEQS"
     gene            complement(5405..8359)
                     /gene="infB"
                     /locus_tag="VGC69_18995"
     CDS             complement(5405..8359)
                     /gene="infB"
                     /locus_tag="VGC69_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY0297421.1"
                     /translation="MSSNTVAQFATELKMPANVLLEQLRSAGVDLKSVDDAVTDSDKA
                     KLLDSLRRAHGGSEGKKITLTRRQTSEIRQADATGRSRTIQVEVRKKRVFVKRDPAEL
                     AAEQADAAAEENSAAVAQALPVEADIQVVDEPAPAPAPQAVEPVAPAAPVAEAVEPAA
                     AAPAVPVAPEPAPEPAVAAAPEPIAAAPQAEPEPESAPASKAAEAPAAPVLEQPEPAQ
                     AEAAAEPAAESVEAPAVEPAPTEAPKNAPRAAAKTASEPRRAAAPAPAAGNDDRRRAA
                     EAEAAALREMLNRPRKVLRAPEPEQAAAAAAALSGTLHKPAGKAAVVKKEAKPGGAPG
                     TKKTIKTAEVASTWTDDASRKKPADKPAANTRDSGWRAGGKGGKGGRNSRNQQSDRRA
                     EPAPQEFIAREIHVPETISVADLAHKMSVKAAEVIKHLMKLGQMVTINQVLDQETAMI
                     VVEELGHVAIAAKLDDPEAFLDEAAPVVSEAELLPRAPVVTVMGHVDHGKTSLLDYIR
                     RAKVAAGEAGGITQHIGAYHVKTDRGMVTFLDTPGHEAFTAMRARGAQATDIVILVVA
                     ADDGVMPQTREAIHHAKAAGVPLVVAINKIDKPDANPDRVKQELVAEQVVPEEYGGDV
                     PFVPVSAKTGAGIDDLLENVLLQAEVLELKAPVDAPAKGLVIEARLDKGRGPVATILV
                     QSGTLHRGDAVLAGASFGRVRAMLDENGKPIHEAGPSIPVEIQGLTEVPAAGDEIMVL
                     SDERKAREIALFRQGKFRDVKLARQQAAKLESMFDNLGEGVQNLPLIVKTDVQGSQEA
                     LVTALTKLSTDEVRVQVVHAAVGGISESDVNLAIASNAVVIGFNVRADQSAKKLAENN
                     GIDLRYYNIIYDAVDEVKAAMSGMLAPEKKEEIIGLVEIREIFSVSKVGNIAGCMVLD
                     GLVKRDAQVRLLRNNVVAWTGYLDSLRRFKDDVKEVKSGFDCGLTLRGYNDIQVGDQL
                     EVFEIKEVARTL"
     gene            complement(8445..9923)
                     /gene="nusA"
                     /locus_tag="VGC69_19000"
     CDS             complement(8445..9923)
                     /gene="nusA"
                     /locus_tag="VGC69_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY0297422.1"
                     /translation="MSREILLLVDALAREKNVTRDTVFTALESALASAMKKRFKDDAD
                     IRVAIDRETGSHEGFRRWLVVPDEAGLQEPDKQELLSDALDLVPGIEVGEYIEEPLEP
                     VEFGRIGAQAAKQAILQKIRDAEREQVLNDFLDRGETIVSGTVKRMDKGDAIIETGKI
                     EARLPRSEMIPKENLRISDRVRAFVLRVDSTLRGQQVILSRTSPEFIRQLFENEVPEI
                     EQGLLEIKAAARDPGVRAKIAVVAYDKRIDPIGTCVGMRGSRVTAVRNELGGEQVDIV
                     LWSDDPAQFVIGALAPANVESILVDEDKHAMDVVVDEENLPKAIGAKGQNVRLASELT
                     GWQINIMTPEESQNRQETERTGLRATFMNKLDVDEEVADILIDEGFTGIEEIAYVPMQ
                     ELLEIESFDEDTINELRARARNALLTEAIAQEERLETAQDLLDLDGMTAELAAKLAER
                     KVHTRDDLAELATDELAEISGLDEKSASDLIMRARAHWFDEE"
     gene            complement(9920..10423)
                     /gene="rimP"
                     /locus_tag="VGC69_19005"
     CDS             complement(9920..10423)
                     /gene="rimP"
                     /locus_tag="VGC69_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY0297423.1"
                     /translation="MADLYAMTQEALAGMDVELVDVERAAMGLLRVTIDKEGGVRIED
                     CEQVSRQLSRVYEVENIDYRRLEVGSPGIDRPLRSEADFLRFADSRIEIKLREAIDGQ
                     KVFTGTLRVPETAGGQATAGEAPAHPVFGLEFEAKKNDIQVLSFTLDDIERAKLDPVL
                     DFKGKKR"
     gene            complement(10725..12473)
                     /locus_tag="VGC69_19010"
     CDS             complement(10725..12473)
                     /locus_tag="VGC69_19010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY0297424.1"
                     /translation="MPEPEGAGAKPADAAAPESEARGRGRKLRTPFRRRRGDAVAAEG
                     GERPEQRPEQRLEPRSEQRPERKSKRGPRKPAGAAGQAAPAASAEAAVPGLDVDPHVL
                     ERETEQALAYLDSDARTEQRLGKYLNSEAVSPKLHKVLAEAGVGSRREMEELIVAGRV
                     SVNGEPAHIGQRVAPNDQVRVNGKPVSRQNTRKPPRVILYHKPAGEIVSHDDPGGRAS
                     VFARLPKLRTGKWLSVGRLDLNTEGLLIFTTSGDMANRIMHPRYGNEREYAVRVLGEM
                     DEATRKSLVEGIELEDGKAAFGALDYLGGDGSNRWYRVTLQEGRNREVRRMFEAVGVT
                     VSRLIRTRFGDVVLPRNLRRGRWEEVDAQLVSALMVQLGLLRDDDGEGRYEKQPTSHE
                     NALPPGFGTMERNGMNGARIGRRGKLQGGGQGSPGSVRSTPSDPFGTGLMITGGYANG
                     HPMGGDKAGSPKTTGSAKAGGGARAGGAKGGAKRGQGGGARKSAGQGPGQGPQGQGPG
                     QGQGKRPARAGGPKAAAGNANGGAGNGGTAGKRPSSAKSKPRKPGGRGDDWQPSGASA
                     HESRLGFLGGRGRNSR"
     gene            complement(12713..13270)
                     /gene="scpB"
                     /locus_tag="VGC69_19015"
                     /pseudo
     CDS             complement(12713..13270)
                     /gene="scpB"
                     /locus_tag="VGC69_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            13788..>14311
                     /locus_tag="VGC69_19020"
     CDS             13788..>14311
                     /locus_tag="VGC69_19020"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0297425.1"
                     /translation="MLYSFNRRSFLASSGALTLGVLVPGFSRAQTLDITSTVFGGVWE
                     KNYRAAIVDRFEQANGAKVNLRLGSSAEWLTNAIVNKAAPEIDLLMLPYPDSIKAIMA
                     DIGIELTPGDIPNIAKIEPIWWEQYKKRGVGLDYTTYGIAYRTDLVKEPIEKWTDLFR
                     PELAGKITLPDIGT"
BASE COUNT         2271 a         4659 c         4733 g         2648 t
ORIGIN      
        1 tcgtgcgccg cgccgagcag gccaagttcg acctggtgtt ctttgccgac acgcccgaca
       61 ccagcgccga cctgcacccc ggaatgatgg tccgcctcga gccgctggtg ctgctgggcg
      121 cgctgtcgat ggtgacgagc aggctgggcc tggtggccac ggtctcgacc acctactcgc
      181 agccctacaa cgtggcccgg gccttcgcct cgctcgaccg catgagcggg ggccggatcg
      241 gctggaacgt ggtcacgggt tcgagcccga acgcggccta caacttcggc aacgaacacc
      301 accccgacca cgggtcgcgc tacgagatgg cgaccgagta cctgaaggtg gccaaggggc
      361 tgtgggacag ctgggaggac gacgcgctgg tggccgacaa ggccacgggc gtgtacatgg
      421 acaccaccaa gatgcacacc ctcaaccatg aggggaagta ttaccaggtc aagggtccgc
      481 tcaatgccag ccgtccgccg cagggctatc cggtgatctt ccaggcgggc gcctcggaca
      541 cgggcaagga ctttgccgcg gccacggccg aggtggtgtt cgccacgcag tcgatcctgg
      601 aggacgcggt ggcgttcgcc aaggacctga aggaccgggt ggagaaggcg ggccgctcgc
      661 gcgattcgct gaagatcatg ctgggggtgg cgccgatggt cggtgatagc tatgaggacg
      721 cgcagcggaa ggtggccgag cttgcccggc tggtcaagcc ggaagtggcg ttgaagacgc
      781 tgtcggaccg gctgggccac gacatgtcga agtttcccct ggacgagccg ctgccggagc
      841 tgccgcaatc gaccatgatg cagggccatg cggtggtgct gaagtcgatc gccaagaagt
      901 acaacatgac gctgcgccag ctgctggact acgcggcggt cagttcgggg caccgactgc
      961 tgatgggcac ggccgagcag gtggccgacg atctgcagct gtggttcgag gcaggagcgg
     1021 ccgacggctt cgtggtgctg ccgtcgttct acccgacgcc gttcgaccgc ttcgtcgacg
     1081 aggtggtgcc gatcctggcc gagcgcggcg tcttccgcaa ggaatacgca ggctcgacgc
     1141 tgcgcgagca cctgggactg gaacgccccg cgcatcccct gaccctgggt cgctgaaccg
     1201 gaagccgcgc gccatggaca aaatcattgc ccaggatgaa ttcagggatg ccgtttcacg
     1261 cctgggcgcc gccgtccacg tcgtgaccac caatggcgaa ggcggactgg cggggtttac
     1321 cgcctcggcg gtctgcagcc tgaccgatac gccggccatg ctgctcgtct gcctgcagaa
     1381 aaagtcctcc gcctacgagt ccgtcaagaa gaacggcgtg gtctgcgtca atatgctggg
     1441 ctcggcgggc ctggaactat gcaaggcgtt cagcggcaag acccccatgg ccgaccggtt
     1501 cgccgatctg gcgtggaaaa gcgccgcgac gggctccccc gtgctgcccg actcgatgat
     1561 cgccttcgac tgcaccgtga cggaattcaa gagcgtggaa acgcacgaaa tcctgctgtg
     1621 ccaggtcagg gaaatcatct acggggacac ccagcaaccc gccctcatct actacaaccg
     1681 cgcgtttcat catctgaatt gacgattccc accgcgcagg cggaacacga cggttagtcg
     1741 atgcggacac cggtgagctg ttcgctcaac tgccagagac ggcccgccgc aaccggatca
     1801 gcggcgcgcg ggcgcacgcc atgcaattcc ggggactcat cggaaggcag ggcttgggcg
     1861 atatcgcagt ccacgcaata aacgccaccc atgccatcga gccggggact cgtcgcgcac
     1921 cagacgctgg tcgcggcgcc ttgctgcggc gtcttcttgt tgttttcagg gtcgatgatc
     1981 ggcttgccat ccttgtcgat gacgtcgctg gtcaaacgcg agattcgcgc cggcgcgtcg
     2041 atttccgagg cggcggccaa ggtgggctac gcttccgaaa gcgccttcag gaacgcattc
     2101 aagcgcgtta cggacatggc gccgggccag taccgacaga ccacggagcg agtgaccgca
     2161 gagccgcgtg gatctcccaa cgccgaaacg catgatttct gaccaggcat tggagaaaac
     2221 aaaaacccgc ctgacggcgg gtttttcatg caagcggcaa aggcttaggc gccttcgcgc
     2281 gaggccttgc ggcgctcgtg ctcggagaga tagcgcttgc gcaggcggat cgacttgggc
     2341 gtgagctcga cgagttcgtc gtcgtcgatg aattccaccg cgtattccag gctcatctgg
     2401 atgggcggca ccaggcgcac ggcttcgtcg gtacccgagg cgcgcacgtt ggtcagttgc
     2461 ttgcccttga tggggttgac caccaggtcg ttgtcgcggc tgtggatgcc gatgatcatg
     2521 ccttcgtaca gcgcctcgcc cgggctgacg aacatgcggc cgcgatcctg cagcttccac
     2581 aaggcatagg ccacggcgtc gccgttgtcc tggctgatga gcacgccgtt gcggcgctcg
     2641 ccgatcgagc cgtcgcgcac cggggcgtac tcgtggaaaa tgtgactcat caagcccgtg
     2701 ccgcgcgtca gcgtgaggaa ctcgttctgg aagccgatca agccgcgcgc cgggatgatg
     2761 tattccaggc gggtacggcc gcggccgtcg ggctgcatgt cttgcaggtc gcccttgcgg
     2821 cggcccagct cttccatcac gccgccctgg tgggcatctt ccacgtccac ggtcagcgcc
     2881 tcgaaaggct cgcacttcac gccgtcgatt tcctggaaca ccacgcgcgg gcgcgacacg
     2941 gccaactcgt agccttcgcg gcgcatggtt tccagcagga tggtcaggtg cagttcgccg
     3001 cggcccgcca cttcgaacac ggtatcgtcg cccgtttcgc gcacgcgcag cgccacgttg
     3061 gccttcagtt cgcggtcgag gcggtcgcgc agctggcggc tggtgacgaa cttgccttcg
     3121 cggccggcca ggggcgaggt attgaccatg aagttcatgg tcagggtggg ttcgtccacg
     3181 cgcagcatgg gcagcgcttc gggcgtgagc gggtcggtga cggtggtgcc gatgttgagg
     3241 tcttcgatgc cgttgatcag cacgatgtcg ccggcctcgg cttcttcgac ctgcacgcgc
     3301 tccaggccgc tgaatttcag cacctggttg atgcggccgc gctgcacggc gccttcgggg
     3361 ccgtcgagga agacgatatc catgccggga cgcatgcggc cgcgggtgat gcggcccacg
     3421 ccgatcttgc cgacgtagtt gttgtagtcc agcgacgtga tctgcatttg cagcgcgccg
     3481 ttggggtcgt cgtcgcgctg cggcacgtat ttgagaatgg catcgaacag aggacgcatg
     3541 tcgcccgaac gcacgtcgtc ggtcatgccg gcgtagcccg acaggcccga ggcgtacacc
     3601 accgggaagt ccagttgctc ttcggtggcg cccagcttgt cgaacagctc gaaagtggcg
     3661 ttgatgacga agtcgggacg cgcgcccgga cggtcgatct tgttgaccac gacgatgggc
     3721 tttaagccca gggccagcgc cttgcgggta acgaagatgg tctggggcat ggggccttcg
     3781 accgcatcga ccagcagcag cacgccgtcc accatggaca gcacgcgctc cacctcgccg
     3841 ccgaagtcgg cgtgtcccgg ggtgtcgacg atgttgatgt gcgtgccttc gtattcgacg
     3901 gcgcagttct tggccaggat ggtgatgccg cgctcttttt cgaggtcgtt ggagtccatg
     3961 acccgttcga ccatcgcctg attttcgcgg aaagtgcccg actggcgcag cagttggtcg
     4021 accagggtag ttttaccgtg gtcgacgtgg gcaatgatgg cgacgttgcg caaagcgcga
     4081 gacataatcg atccttaggt tatttgacag gcagcgggga aagcccccac gccgcacctt
     4141 cggtttgctg cccctcgggg gggccgcttt cgccttggga gcggcccggc ggcaaaaggc
     4201 ccgccggcaa ttcgttcaaa gcaagcagtg ccagcttggg atcgggcatg gcctgcaggg
     4261 tatccaacgt cacggcgcgc tccagcgtaa acggcccggc atgcgtgcgc cgcagcgccg
     4321 ccaggtgcgc gtaacacccc aaggcgcggc caatgtcctg ggccagcgtc cggatatacg
     4381 tgcctttgct gcaggccacc tcgatcacgg cctgcatggc ccgggttgac gcaaacccgg
     4441 aaagcagttc gttgcgatag atggtgatct gccgcggtgc gcgttcgagc tcgatgccct
     4501 ggcgcgcata ttcgtacagc ggtttgccgt cgcgcttgag cgccgaatac atgggcggaa
     4561 tctgttcgat cttgcccatg aaacgtgaca gcacatcgcg cagcgcctgc tcctccacac
     4621 cggcaaaacc ttccgccgcg cgggccgtga cgatgccggt caggtccccg ctatcggttt
     4681 cctcgccgaa ctgcagcgtg gcgaggtaag tcttgtcggc gtcgagcatg gcgccggaaa
     4741 tcttggtggc acggcccatg cagcacacca gcaggcccgt ggcaaacggg tccagcgtgc
     4801 cggtatggcc ggccttggcc gcatccaccg tgcgcttggc gcgctgcagg gcgtgattgc
     4861 tcgacaaacc caccggcttg tccagcaaaa gcaccccgtc gagcggctgc ccgcgtctct
     4921 tggccatcgt cgcaatccga aaaaaatcgc cgacagcccc tcaggactgt tcctcgggct
     4981 cgtcggggac accggatgcc gcgcccggat tgtcgcgcgt ggcgcggtcg atcagcgacg
     5041 acatttcgat ggcacgctcg gtctgcggat cgtggtagaa acgcaacgta ggcacggtat
     5101 gaatgtgcag cagcttgtac agcagggaat gcagccagcc cgacttctcg ttgagcaagg
     5161 cggccgtcgc atcgggctcg gcgcccagta cggtgaaata taccttggcg tgcgcatagt
     5221 cggccgagag atccacgccc gaaatcgtga tcagtcccga gcgcgagaca tcgacctcgc
     5281 gctggacgat ctcggccaga tccttctgga tctggtcggc cagccgcaga ttgcggccgg
     5341 ggatggactt ggatttatgg cggctcatga ctggctgata tgcggaaagc ggcggtgcac
     5401 caccttacag cgtgcgggcg acttccttga tctcgaagac ttccagctgg tcgcccacct
     5461 ggatatcgtt gtagccgcgc agggtcaggc cgcaatcgaa gccggacttg acttccttga
     5521 cgtcgtcctt gaagcggcgc agcgaatcga ggtagcccgt ccaggcaacc acgttgttgc
     5581 gcagcaggcg gacctgcgcg tcgcgcttga ccaggccgtc gagcaccatg caaccggcga
     5641 tgttgccgac cttggacacc gagaagatct cgcggatctc gaccaggccg atgatctctt
     5701 ccttcttctc gggagccagc atgcccgaca tggccgcctt cacttcatcc acggcgtcgt
     5761 agatgatgtt gtagtagcgc aggtcgatgc cgttgttctc ggccagcttc ttggcgctct
     5821 ggtcggcgcg cacgttgaag ccgatgacca cggcgttcga ggcgatggcc aggttgacgt
     5881 cggattccga gatgccgccc acggccgcgt gcaccacctg cacccgcacc tcgtcggtgg
     5941 agagcttggt gagcgcggtg accagcgctt cctgcgagcc ctgcacatcg gtcttgacga
     6001 tgagcggcag gttctgcacg ccctcgccca ggttgtcgaa catggactcg agcttggcgg
     6061 cctgctggcg ggccagcttg acgtcgcgga acttgccctg gcggaacagc gcgatttcgc
     6121 gcgccttgcg ctcgtcggac agcaccatga tctcgtcgcc ggcggcgggc acttcggtca
     6181 ggccctggat ttccaccgga atggaggggc ccgcctcgtg gatgggcttg ccgttctcgt
     6241 cgagcatggc gcgcacgcgg ccaaagctcg cgccggccag caccgcgtcg ccgcggtgga
     6301 gcgtgccgct ctgcaccaga atggtcgcca ccgggccgcg gcccttgtcc aggcgcgctt
     6361 cgatgaccag gcccttggca ggcgcgtcca ccggcgcttt caattccagc acctcggcct
     6421 gcagcagcac gttttcgagc agatcgtcga tgccggcgcc ggtcttggcc gaaaccggca
     6481 cgaagggcac gtcgccgccg tattcttcgg gcaccacctg ctcggccacc agttcctgct
     6541 tgacgcggtc ggggttggcg tcgggcttgt cgatcttgtt gatggcgacc accagcggca
     6601 cgccggccgc cttggcatgg tggatggctt cgcgcgtctg cggcatgacg ccgtcgtcgg
     6661 ccgccaccac caggatgacg atgtcggtgg cctgggcgcc gcgcgcacgc atggcggtga
     6721 acgcctcgtg gcccggggtg tcgaggaagg tcaccatgcc acggtcggtc ttcacgtggt
     6781 aggcgccgat gtgctgcgtg atgccgcccg cttcgcccgc ggcgaccttg gcgcggcgga
     6841 tgtaatccag cagcgaggtc ttgccgtggt cgacgtggcc catgacggtg accaccggcg
     6901 cgcgcggcag cagttcggct tcgctgacaa cgggcgcggc ttcgtccagg aaggcctcgg
     6961 ggtcgtcgag cttggcggcg atggcaacgt ggccgagctc ctcgaccacg atcatggcgg
     7021 tttcctggtc gagcacctgg ttgatggtga ccatctggcc cagcttcatg aggtgcttga
     7081 tgacctcggc cgccttgacg gacatcttgt gcgccaggtc ggccacgctg atggtctcgg
     7141 gcacgtggat ttcacgcgcg atgaattcct gcggcgcggg ttcggcgcgg cggtcggact
     7201 gctggttgcg ggagttcctg ccacccttgc cgcccttgcc gccggcacgc cagccgctgt
     7261 cgcgcgtgtt ggccgcgggc ttgtcggccg gcttcttgcg cgaagcgtcg tccgtccagg
     7321 tggaggccac ctcggcggtc ttgatggttt tcttggtgcc gggtgcgccg ccaggcttgg
     7381 cttccttctt gacgacggcg gccttgcccg cgggcttgtg cagcgtgccc gacaaggcag
     7441 cggcagcggc ggcggcctgt tcgggctcgg gcgcgcgcag caccttgcgc ggacgattca
     7501 gcatctcgcg cagggcggcc gcttcggcct cggcggcacg gcggcggtcg tcgttgccgg
     7561 cagccggcgc gggggccgcg gcgcggcgcg gctcggacgc cgtcttggcg gcggcgcgcg
     7621 gcgcgttctt gggcgcttcg gtgggcgcag gctcgacggc cggcgcttcg acggattcgg
     7681 cagcgggttc ggccgcggct tcagcctgcg cgggctccgg ctgctcaagc accggggcgg
     7741 caggcgcctc ggcagccttg gacgcaggcg cggattcggg ctcgggttcg gcctgcggcg
     7801 cagcggcaat aggctcgggg gcagcggcaa cggccggctc gggagccggc tcgggcgcta
     7861 ccggcacggc cggcgcggcg gccgcgggct cgacggcctc ggcaaccggt gcggcgggcg
     7921 ccacgggttc gaccgcctgc ggcgcgggtg ccggcgcggg ttcgtcgacg acctggatgt
     7981 cggcttcgac gggcagcgcc tgcgcaacgg ccgcggaatt ttcttcagcc gcggcgtcgg
     8041 cctgctcggc ggccagttcg gcgggatcgc gcttgacgaa gacgcgcttc ttgcgcactt
     8101 cgacctgaat ggtgcgcgaa cggccggttg catcggcctg gcggatttcc gaggtctgac
     8161 ggcgggtcag cgtgatcttc ttgccttcgc tgccgccatg ggcgcggcgc agcgagtcga
     8221 gcaacttcgc cttgtcgctg tcggtgacgg catcgtctac cgacttgagg tcgacgccag
     8281 ccgaacgcag ctgctccagc agcacgttgg caggcatttt cagttcggtg gcgaactggg
     8341 cgacggtatt actcgacatt aggctatctt cctttgcaac tatcttctgt atgccggccg
     8401 cgcaaagcgc gacgcggcga ttctttagcg acggtccatg gtcttcactc ttcgtcgaac
     8461 caatgggcgc gggcacgcat gatcaggtcg ctggccgact tctcgtccag accgctgatt
     8521 tccgcgagct cgtcggtggc gagttcggcc aggtcatcgc gcgtgtgcac cttgcgctcg
     8581 gccagcttgg cggccagctc ggccgtcatg ccgtcgagat cgagcaggtc ctgcgcggtt
     8641 tccaggcgtt cttcctgggc aatggcttcg gtcagcagcg cgttgcgggc acgggcgcgc
     8701 aactcgttga tggtgtcttc gtcgaacgac tcgatctcca gcagctcctg catgggcacg
     8761 taggcgatct cctcgatgcc ggtgaaacct tcgtcgatca ggatgtcggc cacttcttcg
     8821 tcgacatcga gcttgttcat gaaggtggca cgcagcccgg tacgctcggt ttcctggcgg
     8881 ttctggcttt cttccggcgt catgatgttg atctgccagc cggtcagctc ggaagccagg
     8941 cgcacgttct ggcccttggc gccaatggcc ttgggcaggt tttcttcgtc gaccaccacg
     9001 tccatggcgt gcttgtcttc atcgaccagg atggactcga cgttggccgg agccaacgcg
     9061 ccgatcacga actgcgccgg gtcatcggac cacagcacga tgtcgacctg ctcgccgccc
     9121 agctcgttgc gcacggcggt gacgcgcgaa ccgcgcatgc ccacgcaggt gccgatggga
     9181 tcgatgcgct tatcgtaagc caccacggcg atcttggcac gcacgcccgg gtcgcgggca
     9241 gccgccttga tctcgagcag gccctgttcg atttcgggca cttcgttctc gaagagctgg
     9301 cggatgaact cgggcgaggt gcgcgagagg atcacctgct ggccgcgcag ggtgctgtcc
     9361 acgcgcagca cgaaggcccg cacgcggtcg ctgatgcgca ggttttcctt ggggatcatc
     9421 tcgctgcgcg gcaggcgggc ttcgatcttg ccggtttcga tgatggcgtc gcccttgtcc
     9481 atgcgcttga cggtgccgga cacgatggtt tcgccgcgat cgaggaagtc gttgagcacc
     9541 tgctcgcgct cggcgtcgcg gatcttctgc aggatggcct gcttggcggc ctgggcgccg
     9601 atgcggccga attccaccgg ttcgagcggc tcctcgatgt actcgcccac ctcgatgccg
     9661 ggcacgaggt cgagcgcgtc ggacagcagt tcctgcttgt cgggttcttg caggccggct
     9721 tcgtcgggca ccaccagcca gcggcgaaag ccttcgtggc tgccggtttc gcggtcgatg
     9781 gcgacacgga tgtccgcgtc atccttgaaa cgctttttca tggccgaagc cagcgcgctt
     9841 tcgagcgccg tgaacacggt atcgcgcgtg acgttctttt cgcgcgccaa cgcatcgacc
     9901 aacagaagaa tttcgcgact catcgctttt tgcccttgaa atccagaacg ggatccagct
     9961 tggcgcgctc gatgtcatcg agcgtgaagc tcagcacttg gatatcgttc ttttttgcct
    10021 caaattccag accgaacacg gggtgcgcag gcgcttcgcc cgcagttgcc tgaccccctg
    10081 cagtctcggg aacacgtaac gtgccggtga ataccttttg gccgtcgatg gcctcgcgca
    10141 gcttgatctc gatgcgcgaa tccgcaaacc gcaggaagtc cgcctcgctg cgcaatggcc
    10201 ggtcgatacc gggcgagccc acttcgagcc gccggtaatc gatgttctcg acctcgtaca
    10261 cccgcgacaa ttgacgggag acctgttcgc aatcttcgat gcgaacgccg ccttccttgt
    10321 cgatggtgac gcgcagcaaa cccatggcgg cacgctcgac gtcgaccagc tcgacgtcca
    10381 tgcccgccag ggcttcctgg gtcattgcat ataaatcagc cacggatttg acaccgaatt
    10441 tgccactgaa tttgccacac cactctgcac atgtaattgc tatctatatt gctacgtata
    10501 ttcaaaaaaa atgggctgtc tgcccagccc accgtattac cgaaagcccg gcacaaaacg
    10561 cgtaccaggc tcggcctcag ttgttctttg cttgcagcgg ccgcgcccaa aaaagtgcgg
    10621 gcgaccaaag acgtcgctga caaacgggaa agtttaccag aaaaacgtaa aaattaccca
    10681 attgttcccg ggtttacccg ggtggggccc cacttgggta ggatttacct gctgttgcgt
    10741 ccccggccac ccaggaaacc caggcgcgat tcgtgcgccg aagccccgct gggttgccag
    10801 tcgtcgccac ggccgcccgg cttgcgcggc ttggacttgg cgctggacgg acgcttgccg
    10861 gccgtgccgc cgttgcccgc gccgccattg gcattgccgg ccgcggcctt gggaccgccc
    10921 gccctggcgg gacgcttacc ctgcccctgc cctggaccct ggccctgcgg accctggcct
    10981 gggccctgcc cggccgactt gcgtgcgccg ccaccctggc cgcgcttggc gccgcccttg
    11041 gcgccacccg ccctggcgcc accacccgcc ttggcgctcc ccgtcgtctt ggggctcccg
    11101 gccttgtcgc cgcccatggg gtggccgttg gcatagccgc cggtgatcat caggccggtg
    11161 ccgaagggat cggacggcgt ggaacgcacc gagccagggc tgccctggcc gccgccctgc
    11221 agcttgccgc gccggccgat gcgcgcgccg ttcatgccgt tgcgctccat cgtgccgaag
    11281 cccggcggca gcgcgttttc gtgcgacgtg ggctgctttt cgtagcgccc ctcgccgtcg
    11341 tcgtcgcgca gcagacccag ctgcaccatc agggcgctga ccagttgcgc gtcgacttct
    11401 tcccagcggc cacggcgcag attgcgcggc agcacgacgt cgccgaaacg ggtgcggatc
    11461 aggcggctga cggtgacgcc caccgcctcg aacatgcggc gcacttcgcg gttgcggcct
    11521 tcctgcaggg tgacgcggta ccagcggttg ctgccatcgc cgcccaggta atccagggcc
    11581 ccgaaagcgg ccttgccgtc ttccagctcg atgccctcga ccagcgactt gcgcgtggcc
    11641 tcgtccatct cgcccagcac gcgcacggcg tattcacgtt cgttgccgta gcgcggatgc
    11701 atgatgcgat tggccatgtc gcccgaggtc gtgaagatca acaggccttc ggtgttcagg
    11761 tccaggcgcc ccaccgacaa ccacttgccg gtgcgcagct tgggcaggcg cgcgaacacg
    11821 ctggcccggc cgcccggatc gtcgtggctg acgatttcgc cggccggctt gtgatagagg
    11881 atcacacgcg gcggctttcg ggtgttctgg cggctgacgg gcttgccgtt gacgcgcacc
    11941 tggtcgttgg gcgccacgcg ctggccgatg tgcgccggct cgccgttgac cgacacgcgg
    12001 ccggccacga tgagctcttc catttcgcgg cgcgaaccaa cgccggcctc ggccagcacc
    12061 ttgtgcagct tggggctaac ggcctcgctg ttgagatact tgcccaggcg ctgttcggtg
    12121 cgcgcgtcgg aatcgaggta ggccagggcc tgctcggtct cgcgctcgag cacgtgaggg
    12181 tccacatcca ggcccggcac cgcggcctcg gccgatgccg cgggagcagc ctgcccggcg
    12241 gcgccggcgg gcttgcgagg gccgcgcttg gatttgcgtt cggggcgctg ctcggagcgc
    12301 ggttcgagac gttgttcggg acgctgttca gggcgctcgc cgccctctgc cgcgacggca
    12361 tcgccgcgac ggcggcggaa gggggtgcgc agtttgcggc ctcggccgcg agcctcggat
    12421 tcaggggcgg cggcatcggc cggcttggcg cccgcgccct caggttcagg caatgacgtt
    12481 ttttcttgtg tatcgggatc ggttgtcact gagacagctc cggaggtcaa acttgagacg
    12541 gcctgtcgtc ggcaggctca tgatcttgtt cagggttggg cgccggggct gcatcgccct
    12601 cgacgtcgtg aatggttgtt tcaactgccg cttcggcccg ggattcgacc gccggctcga
    12661 cagccgctgc ttctgcttct gccgcctgcg cctggccagc gggctcgttg gccgcctcgc
    12721 ccaggtccag gctggccagc acggcggccg ggtcggtcga ttcaatcgac ggcagctcgt
    12781 ccagcgcgcg caggcccagg tcatcgagga actggcgcgt cgtgccgaag agcgccgggc
    12841 ggcccggggc gtcgcggtgg ccgatggcct cgatccagcc gcggtcttcc agcgccttga
    12901 cgatctgcga ggacaccgtc acgccgcgaa tatcttcgat gtcgccgcgc gtcaccggct
    12961 ggcgccaggc gatgatggcc agggtttcga gcaccgcgcg cgaatacttg ggcggctttt
    13021 cggggttgag ccgctccagg tagcgttgca tctgcggccg gctctggaaa cgccagccgg
    13081 aagccagttg caccagttcc aggccacgcc cggcccattg ggcctggagc gcttccagcc
    13141 actgctgcaa cgcagcgttg tcgatttccg attcttcgcc gaacagcttg cgcagctcgg
    13201 cggcctgcat cggttgggat gcgcacagca gcgcggtttc caggaccagt agcgcctcgt
    13261 tgtcactcat cgatatatca aaacccgaag gttgtaggtt caaacctgcc cccgcaacca
    13321 gatccagcct ggcgcttcgc gcggccagac cttggccgca ccggcatgct ctcggccagg
    13381 ccgaaagagc gaaatcaaga acgattgaac gatgtccgta cggggacaac ggcgcgccac
    13441 ggcgtttttt gaaatttgcg ggcggcggat tgccacgtac ctgccgtatg taaaccagcg
    13501 cactgggcac cccgcatcag gcacccatag ggcggttcac cggtgccatg cactccggaa
    13561 aaagctgcga ggcaggcaga cggcatgaaa gctgggctcc attatatacc cgtataggct
    13621 ccagacccaa gattgttctg cccgacacgc tagaaaagcg cggcgacccg ccctgccccc
    13681 gggtgcgcgc atcctccacg cgacatcctg ggccagcaaa agctagaagt tcacgatgat
    13741 aaattttata ctttataaaa acacaaatac catgacaagg gcacctcatg ttgtactcct
    13801 tcaatcgaag atcctttctt gcatcaagcg gcgcgctgac gctgggcgtc ctggtccccg
    13861 ggtttagccg cgcgcagacc ttggacatca cctccacggt attcggcggc gtctgggaaa
    13921 agaattatcg cgccgccatc gtcgatcgct tcgagcaggc caacggcgcc aaggtgaact
    13981 tgcggctcgg ctcgtcggcc gaatggctga ccaacgcaat cgtcaacaag gcagcccccg
    14041 aaatcgatct gctgatgctg ccctatcccg acagcatcaa ggccatcatg gccgatatcg
    14101 gcatcgagct gacgcccggg gacatcccca acatcgccaa gatcgaaccg atctggtggg
    14161 agcaatacaa gaagcggggc gtcggcctcg attacacgac ttacggcatc gcctaccgca
    14221 ccgatctggt caaagagccg atcgagaaat ggacggatct gttccgcccc gagctcgcgg
    14281 ggaaaatcac cctcccggac atcggcacct g
//
LOCUS       DASWPO010000356        20922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2408481, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000356 DASWPO010000000
VERSION     DASWPO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 20922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20922
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408481"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VGC69_19025"
     CDS             complement(<1..547)
                     /locus_tag="VGC69_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0297426.1"
                     /translation="MNPSTLIGFALGIVTVILVVAFSAIDSWAYLNLPGLAVVIGGTL
                     AALLISYPMREVLRIPKLLRAVFGNDKLDSQRDIDELSAMAQLWMNDDVHKVEEALKK
                     VANPFLRTGVQLIIDNTPEEQIIELLQWRIARLRAREQAESHMFRTMASFAQAFGMLG
                     TLIGLVDLMTVLGAGGMDIIGH"
     gene            complement(674..1501)
                     /locus_tag="VGC69_19030"
     CDS             complement(674..1501)
                     /locus_tag="VGC69_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0297427.1"
                     /translation="MTAADTAASGIAWADIHTPTRDLLRLEYRWIAPERRDAPLLVFL
                     HEGLGSIAIWRDWPEQVCAAAGCRGLVYSRYGYGQSTPRPAEEKLTPRYLHEQAHEVL
                     PALLAALGVDAEADKPVFYGHSDGGSIALLYAARYPQAVAGIAIAAPHIFVEDITVEG
                     IRQARELYLTTDLRGRLARYHADVDSAFWGWNDAWLDPVFRDWNIEREVRDIRCPILA
                     MQGIGDHYGTLEQIRGIARLAPQTRLLEIPACGHTPHKDQPKIVTRALVDFLRSLPR"
     gene            complement(1576..2352)
                     /locus_tag="VGC69_19035"
     CDS             complement(1576..2352)
                     /locus_tag="VGC69_19035"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY0297428.1"
                     /translation="MADTMHLGKGEIVAASLEKMETALHGAKMTRKEAVAETCRILFA
                     FGHDSALSGQITARAERPGTYWTQQLGLGFDEITADNLLLVDQDLNVLEGQGMPNPAN
                     RFHSWLYRARADIQCIVHSHAPHVSALAMLEVPLAISHMDTCLLYEQCAFLPKWPGIP
                     VGNEEGEIISTAIGDRRALLLAHHGLLVTGKSVEEACVMGVMFERAARLQLLAMAAGD
                     IQPIDPELGREAQRWVSTQKRYEATFAYYSRRARRELGQA"
     gene            complement(2397..3371)
                     /locus_tag="VGC69_19040"
     CDS             complement(2397..3371)
                     /locus_tag="VGC69_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY0297429.1"
                     /translation="MNHALRRMLQSVLFCSAALPALSHAAGYPDHAITMVVPFGAGGI
                     SDLVARATGRALSEQVGQSVIIENKPGAGGNIAADYVKRARPDGYTLLFAPVGLLAVN
                     PHTADGAPFDNSKDFSYLSLVASTPHVIVVNPSVPAHNLEELIALARRKPEGLTFGTA
                     GIGSSPYQGMVIMEESTGVKFLHVPFKSGIESVTNVVSGQVDMTFEATPVVMPFVNAR
                     KMRAIAVAHTSRIDSAPDLPSTAELGHPELLSGSAAGVVGPAGLSDDVVKILSTALTQ
                     AVAKPEFKALLKAQGTAAESSTPQGFKTLIAQEDKRWARLLAPSVAGK"
     gene            3527..4105
                     /locus_tag="VGC69_19045"
     CDS             3527..4105
                     /locus_tag="VGC69_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0297430.1"
                     /translation="MDDKENINVVGERLAVLRKLNALTLEEVANRATLTKSYLSKLER
                     GLSSPTIATVLKLARALDVSLDQLIGGTSPSREILHQHAADRVPFSPSSAREGYTYEA
                     IATDRPDKVMMPFIMAPPFTLEEQELMSHPGEELIYVVSGEIEVVFADRTVRMAAGDS
                     LYFNASLAHRSRSLGEVLAQALVVVSDPARRR"
     gene            complement(4111..5364)
                     /locus_tag="VGC69_19050"
     CDS             complement(4111..5364)
                     /locus_tag="VGC69_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEY0297431.1"
                     /translation="MQSDDTLLYGAQVMAWADKLAAWSEQDGNLTCTYLTPAHRAAAR
                     QLGEWMREAGFDTVRHDAVGNVVGRYKADAAVATPGLVVTGSHYDTVRNGGRYDGRLG
                     ILLPMAVVADLHRRGERLPYDFEVVGFAEEEGARYGTSFLASSAYIGRYSPAWLDLAD
                     AKGVTLRQAMDEAGLDPAGIAQAAIDPALIKHYFEVHIEQGPVLLDAGLALGVVTSIA
                     GSVRRRLLLKGRAGHAGTTPMHMRRDAACAAAEIALYVEARCGQAETLVGTVGMLEVP
                     SGSVNVIPGACRMSLDIRAADDATRDAAMADINRKIDEICTRRHVSVEDTQPAMTVPA
                     APCSAPHRALWRDVLSGLGLPDFELLSGAGHDAMTLEKITPVSMLFTRCGNGGISHNP
                     LEIITADDAELAGRAVEAFLRKLAD"
     gene            5666..6385
                     /locus_tag="VGC69_19055"
     CDS             5666..6385
                     /locus_tag="VGC69_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0297432.1"
                     /translation="MSNIQAIRGNHLLSVLSEVEWARIGPHLVLVDMPLGQVVYESGD
                     RLDHVYFPTTSIISLLYVMEDGASGEIAIVGNEGVVGIALFMGGETTPSRAVVQSAGY
                     AYRLNSHILKEEFHRAGPMQRLLLRYTQALITQMAQTAVCNRHHSIDQQLCRWLLLSM
                     DRLPSNKLKMTQELIANMLGVRRPGVTEAAMKLQDAGLIRYSYGNIEVLDRPGLEHRV
                     CECYAVVKREFDRLLPDLRAL"
     gene            6523..6957
                     /locus_tag="VGC69_19060"
     CDS             6523..6957
                     /locus_tag="VGC69_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0297433.1"
                     /translation="MKKHGILLLQWIVVLSATVTGGAALAADEMLPATQHAGAVSYVS
                     GGIGLDQSKALQRAMHNYPLVLTFVRARKNDREYLANVAVTIKDGQGNTVLDAASDGP
                     FMLVSLPNGRYTVSASFQGKSQQHAVAVSRSQHVRQTFSWPR"
     gene            6958..7875
                     /locus_tag="VGC69_19065"
     CDS             6958..7875
                     /locus_tag="VGC69_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEY0297434.1"
                     /translation="MPAYGHSLYLLPFDHRNSYVSDLFHFGFPLDARQQARVEDSKQV
                     IYEGFLAAVAHAVPERCAGILVDEAFGAGILRDARGRGYATAVSVERSGSDEFQFEYG
                     DDFAAHIEAVDPTFAKVLVRYNPEDDAAMNLRQAARLRALTQYCQRTHRLLMFELLVP
                     ATGRQLQGAHGDRSAYDLDVRPALMLAAIAALQDAGVEPAIWKIEGLDRSEDCRSMVA
                     AARRGGRGHVGCIVLGRGADEKKVIDWLETAAQVDGFIGFAVGRTSFYDAIADYVAGK
                     ATREQAAGRIAGQYAKWAAIFEQARKAGP"
     gene            complement(7897..9300)
                     /gene="zwf"
                     /locus_tag="VGC69_19070"
     CDS             complement(7897..9300)
                     /gene="zwf"
                     /locus_tag="VGC69_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY0297435.1"
                     /translation="MPPFKSDAFVFFGATGDLASKQIFPALQAMTRHGRLDIPVIGVG
                     RSALGREQFIAHARDSIDKHGVPDDDVFKTLAPRLQYISGDLLDSSTYRQLRAALGPA
                     RHPLFYLAIPPGMFGRVAKGLAESGYAHDARVIVEKPFGHDLASAQALNLLLTQSFPA
                     SAIFRIDHYLGKEPIQNLLYFRFANAILEPLWNREHVASVQVTMAESFGVQGRGSFYE
                     GVGTIRDVVQNHLLQVVSLLAADAPADGRPDALSDAKSRVFAAMRPIAPGEAVRGQFR
                     GYLDEKDVAPRSNVETYIALRLHIDNERWAGVPFYLRAGKRLAATSTEILVMLKPPPH
                     EIFDAMTSEQLNYFRFRISPDVLISIGTRVKIPGEAMLGQEVELVAHRHPGDEMTPYE
                     RLLDDAILGNAALFARYDCIEAAWRTVMPILGDSVPLVTYEPQTWGPEQADRIMTGDG
                     HWHNPAPTAAAPPMPEP"
     gene            complement(9303..10331)
                     /gene="gnd"
                     /locus_tag="VGC69_19075"
     CDS             complement(9303..10331)
                     /gene="gnd"
                     /locus_tag="VGC69_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY0297436.1"
                     /translation="MQIGMIGLGRMGSSMVRRLLKKGHECVVHDTHAELVNTLRQRGA
                     AGSDTLQDFVSRLEKPRTIWLMLPAAAVDAELAALLALLEAGDVIIDGGNSYYHDDIR
                     RSAELRAKGIHYVDVGTSGGIFGLERGYCLMIGGEPSVVQSLAPIFAALAPDAADAPA
                     APEPAAGAGSAQRGYLHCGPHGAGHFVKMVHNGIEYGLMAAYAEGFNILRNANAGSRA
                     DAADAETTPLRKPEHYQYEFDLPEIAEVWRHGSVIGSWLLDLTAGSLRDDPLLTQYAG
                     RVSDSGEGRWTIIAAIDEAVPAPVLSAALYTRFSSRGDADFADKILSAMRNSFGGHLE
                     KPATRSDR"
     gene            10557..10775
                     /locus_tag="VGC69_19080"
     CDS             10557..10775
                     /locus_tag="VGC69_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY0297437.1"
                     /translation="MRNIRTIIVTGMIVPAMLVLIAGCGGMSRRGQDTAVGAGVGGVA
                     GAVLTGGSVLGTVGGAAVGGVVGNQVGR"
     gene            10812..11504
                     /locus_tag="VGC69_19085"
     CDS             10812..11504
                     /locus_tag="VGC69_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0297438.1"
                     /translation="MPGPDDVVLLFDCDNTLFDNDAVLEDLRACMVREFGKTDSDRYW
                     TIFEALRLELGYVDYLGALQRYRRKDQSDTRVMRMSSFLIDYPFASRVYPGALDALRR
                     LRALGKTVIVSDGDVVFQPHKLKHAGLWDEVQGRVLIYIHKETMLDEIERCYPARRYV
                     MVDDKLRVLSAMKQVWGNRLTTVFPRQGHYALDAAAIAAYPPADVVIEHIGELDEAVL
                     AFQPGVDRAGRI"
     gene            11517..13190
                     /gene="pgi"
                     /locus_tag="VGC69_19090"
     CDS             11517..13190
                     /gene="pgi"
                     /locus_tag="VGC69_19090"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY0297439.1"
                     /translation="MKPISQRSLPLGAAAVSLTERPAWQALMAHYRQIRNLHLRDLFA
                     EDPSRSERMTLEGAGLYFDYSKHRITGETIALLLRLARECGLAQRIDAMFSGDAINAT
                     EQRAVLHVALRAPAGEHILVDDADVVPGVHAVLERMAVFCEDVRSGRWRGHTGRPIRN
                     VINIGVGGSGLGSEMACEALRHYSQRNMTFRFISNVDGTDFVENTRDLDPGETLFIIC
                     SKSFTTTETLSNARLAREWSLRALGDESAIRHHFVAVSTNAQGVADFGIDMAYMFGFW
                     DWVGGRYSMDSAGGLSIMMSIGAENFQALLAGFHSMDQHFRTTPFERNLPVLMGLLAV
                     WNSNFFGAETVAVLPYEQYLKRFPAYLQQLAMESNGKHVSSDGTRFAYGTGPIYWGEP
                     GTNGQHSFYQLLHQGTHIVPCDFIGFGRSLNPLGDTHDFLMANLFAQTEALAFGRTAA
                     QIEADGTPSWLAPNLVCEGNRPSSTILAESLTPETLGQLVALYEHSVFTQGTIWNIDS
                     FDQWGVELGKKLARKILPELQGAGPAELDHDSSTNALIRKYRHLRTPSP"
     gene            13217..14872
                     /gene="pgm"
                     /locus_tag="VGC69_19095"
     CDS             13217..14872
                     /gene="pgm"
                     /locus_tag="VGC69_19095"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEY0297440.1"
                     /translation="MRVSPLAGKPASRSILVNVPRLITAYYSDRPDPAIPEQRVVFGT
                     SGHRGSSLACSFNEWHVLAITQAICLYRAQRGIAGPLFIGIDTHALSEPAYASALEVL
                     AANDVEVMIAQNGEYTPTPAVSHAIIAYNRGRSAGLADGIVVTPSHNPPESGGFKYNP
                     PNGGPADSIVTGWIEAAANRLLENQLKGVRRTPLSKAMRASTTHRHDFLNAYVGDLVH
                     VIDMDVMRGAPIHLGVDPLGGAGVHYWEPIAARYGLNLTVLSDVVDPTFCFMSVDWDG
                     RIRMDPSSSYAMQTLIGQRDHFDVAFACDTDHDRHGIVTRYGGLLPPNHYLSVLVDYL
                     YAHRPRWRADAAVGKTLVSSQMIDRVAEKRGRALYEVPVGFKWFADGLLDGSLGYAGE
                     ESAGASCLRLDGTAWTTDKDGMVPALLAAEMTIRTGRDPAELYQDLTEQLGKPVERRV
                     QAAAMAPQRALLARLSPAQFSHTELADEKIERVLDHAPGNGAAIGGIKVATKNGWFAA
                     RPSGTEDIYKIYAESFLGEDHLTRIVDEAQAMVDSALSRVPLP"
     gene            complement(14864..16915)
                     /gene="tkt"
                     /locus_tag="VGC69_19100"
     CDS             complement(14864..16915)
                     /gene="tkt"
                     /locus_tag="VGC69_19100"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0297441.1"
                     /translation="MDTPTLDALCVNTLRFLAIDAVQKANSGHPGLPLGAAPMAYALW
                     SRFLKHNPANPAWFDRDRFVLSAGHGSMLLYSLLHLTGYDLPLEEIKRFRQWGSRTPG
                     HPERGLTPGVETTTGPLGQGMANGVGMAIAEAYLAARYNRPGFALVDHHTYGIVSDGD
                     LMEGVASEAASLAGHLQLGKLIYLYDDNQVTLSAGTDITFTEDRARRFEAYGWHTQCV
                     EDGNDLAAIGQALGHARAETQRPSLILVRTHLGYGSPNKQDSYKAHGSPLGAEETRLT
                     KQNLGWPTEPDFHIPDAALAHFRRALAQGSQQEAGWNTLLSSYAQAFPLLAEELRQVM
                     RGGLPDGWDRGIPIFPADAEGIATRVASGQVMNAIAPYLPTLLGGSADLDPSTFTALS
                     ELGDFAAPGANAHDRQGSGGGGWSRAGRNVHFGVREHAMGAVLNGLAAHGGIVPFGAT
                     FLIFSDYMRPPIRLAALMHLHVIFIFTHDSLALGEDGSTHQPVEQLASLRSVPGLNVI
                     RPADANETAVAWRIALETNDRPTALVLTRQNVPTLDRTWYAAADELRYGGYVLADAPG
                     GKPALILIATGSEVALAMAARERLLAQHIAVRVVSLPCWTLFDAQAQAYRDAVLPPSV
                     SARLAIEAGVPQGWHRYVGEHGGILGMDRFGASAPADVLLREYGFTVDNVCARALALL
                     G"
     gene            complement(16924..17514)
                     /locus_tag="VGC69_19105"
     CDS             complement(16924..17514)
                     /locus_tag="VGC69_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEY0297442.1"
                     /translation="MLRRKFMWSAPGSVLAVGFAIAGCTTTGASDVKTQGQQTDKRRT
                     INAGVDDTLTRLYAAAKGSHELVNKAHGVLVFPSVIDAGFIVGGQYGEGALRISDRTA
                     GYYSTVTGSIGWQIGAQSRAIIFLFMTDESLNQFRKADGWSAGGDASVAVLKVGANGA
                     VDTSTAVGQVNAFLLTNSGLMAGVSLEGTKVSKLVL"
     gene            complement(17660..17986)
                     /locus_tag="VGC69_19110"
     CDS             complement(17660..17986)
                     /locus_tag="VGC69_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297443.1"
                     /translation="MKLSNALSPARDSILKKWPAAAGLAIALACLLATPPLHAQGHRQ
                     GGQNHGQNRGQRHVQRQPVRQIHRQDNRRRGNYYQGYPGYVYGAPPIIYGPQAAPGIN
                     LFLPLR"
     gene            complement(17989..18459)
                     /locus_tag="VGC69_19115"
     CDS             complement(17989..18459)
                     /locus_tag="VGC69_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY0297444.1"
                     /translation="MQRTLLTPWPRTAIAFAVILLLGTAAALSNPVHAASPTTHAMPV
                     TAASSVDTRINSLHSRLQITATQEPLWQSIAQVMRDNAAALDALRQSRTADKSNMNAV
                     DNLRAYGEAIDAHADGIKKLETPFEALYASMSDAQKRNADLIFRDENHMMKKKG"
     gene            complement(18471..18758)
                     /locus_tag="VGC69_19120"
     CDS             complement(18471..18758)
                     /locus_tag="VGC69_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEY0297445.1"
                     /translation="MEYSKLIAATLATLALGACTGPPGPQGNTGNTGYTGRTGQRGDS
                     GYSGATGATGATGDTGATGDTGATGNTGARGNNGARGATSTDGAVLIVPTR"
     gene            complement(18905..19978)
                     /gene="tal"
                     /locus_tag="VGC69_19125"
     CDS             complement(18905..19978)
                     /gene="tal"
                     /locus_tag="VGC69_19125"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY0297446.1"
                     /translation="MKATTQLHALGQSLWLDNMTRDLLTSRTLEHYRDELCVTGLTSN
                     PTLFDEAIRNTSAYDKDIDMKIREGKSLEDLFFDLAIDDLTRAATLFRLIHEASHGVD
                     GWVSLEVSPLLADDTAGTIEKARQLHASAQCDNLFIKVPGTTAGILAVEELIFAGVPI
                     NVTLLFSQDHYLAAADAYARGIQRRIDADLDPKVHCVASIFISRWDKAVQASVPSALH
                     NLLGIAIAKQTYKAHREWLATPQWRKLADHGALPQRLLWASTGTKDPALPGGYYVEAL
                     AAPHTINTMPEKTLRQFAEQGEVNGAMREDGGNAEAILAEFALVGVDIVALAAQLQNE
                     GRQSFTRSWNNLMRVIATKGKSS"
     gene            20158..>20922
                     /locus_tag="VGC69_19130"
     CDS             20158..>20922
                     /locus_tag="VGC69_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY0297447.1"
                     /translation="MNQIPLFLSLVFIVAGAASSQWINVYLGPVLIVIGVLIGLSSKV
                     ANVWEKFVILRLGKLQSVKGAGFFMIIPVLDRIVAIIDERIQTTAFNAEQALTKDTVP
                     VNVDAIIFWHVSDAQKAALSITDYRQAIDRVSQTSLRELIGSSMLSMLLSDRAAADSR
                     LRTEIEGKTSAWGIAVSSVEIRDIAIPVALQDAMSRQAQAEREKQARVILGSAEAAIA
                     ANFVEAARIYENQPGALQLRAMNIIYETTKERGATIL"
BASE COUNT         3565 a         6386 c         7119 g         3852 t
ORIGIN      
        1 gatggccgat gatgtccatg ccgcccgcgc ccagcacggt catgaggtcg accaggccga
       61 tcagggtgcc cagcatgccg aaggcctgcg cgaagctggc catggtgcgg aacatgtggg
      121 actcggcctg ctcgcgggcg cgcagccggg cgatgcgcca ttgcagcagc tcgatgatct
      181 gctcttcggg cgtgttgtcg atgatgagct gcacgccggt gcgcaggaag gggttggcca
      241 ctttcttgag cgcttcctcg accttgtgca cgtcatcgtt catccacagc tgcgccatgg
      301 cgctgagttc gtcgatgtcg cgctggctgt cgagcttgtc gttgccgaac acggcgcgca
      361 gcagcttggg gatgcgcagc acttcgcgca tcggatagct gatgagcagg gcggccaggg
      421 tgccgccgat caccacggcc aggccgggca ggttcaggta ggcccagctg tcgatggccg
      481 agaaggccac caccaggatg acggtgacga tgccgagggc gaagccgatg agggtagacg
      541 ggttcatgca ggcgattcta gccagcctgg cggccgggca atcgtcggga aaaaggccga
      601 aaaccttggg gtttcgatac ggtcgtcgat acggtcgtcg acgcggccgg acagccggcc
      661 gaaaggggcc ggatcagcgg ggcaggctgc gcaagaaatc gaccagcgcc cgggtgacga
      721 tcttgggctg gtccttgtgc ggcgtgtggc cgcaggcggg aatctccagg agccgggtct
      781 gcggcgccag ccgggcaatg ccgcggatct gttccagcgt gccgtagtgg tcgccgatgc
      841 cctgcatggc caggatgggg cagcggatgt cccgcacctc gcgctcgatg ttccagtcgc
      901 gaaagacggg gtcgagccag gcgtcgttcc agccccagaa ggccgagtcc acgtcggcgt
      961 ggtagcgggc cagcctgccg cgcaggtcgg tggtcaggta gagctcgcgg gcctggcgga
     1021 tgccttcgac ggtgatgtct tcgacgaaga tgtgcggcgc ggcgatcgcg atgcccgcca
     1081 cggcctgcgg gtagcgcgcg gcatatagca gcgcgatcga gccgccgtcg ctgtggccgt
     1141 agaaaaccgg tttgtcggcc tcggcgtcca cgcccagggc cgccagcagg gcgggcagca
     1201 cttcatgggc ctgctcgtgc agatagcggg gcgtgagctt ttcctcggcg ggccgcgggg
     1261 tggactgccc gtagccgtag cgcgaataca ccaggccccg gcagccggcg gcggcgcaga
     1321 cctgctcggg ccagtcgcgc cagatcgcga tcgaccccag gccctcgtgc aggaagacca
     1381 gcagcggcgc atcgcggcgc tcgggggcta tccagcggta ttccagcctg agcaggtcgc
     1441 gggtcggcgt gtggatgtcg gcccaggcaa tgccggaggc ggcggtatcg gcggcagtca
     1501 tggcgctagc atacaaaatt acccgcgccg gcgtcccggc gcggcaccgg tggccgcgcc
     1561 gcctggccgg gcggctcagg cctggcccag ctcgcggcgg gcccggcgcg agtaataggc
     1621 aaacgtggct tcgtagcgct tttgcgtgct gacccagcgc tgcgcctcgc ggcccagttc
     1681 cgggtcgatg ggctggatgt cgccggcggc catggccagc agctgcaggc gcgccgcgcg
     1741 ctcgaacatc acgcccatca cgcaggcttc ttccacgctc ttgcccgtga ccagcaggcc
     1801 gtggtgggcc agcagcaggg cgcgcctgtc gccgatggcg gtcgagatga tctcgccctc
     1861 ttcgttgccc accgggatgc ccggccactt gggcaggaaa gcgcactgct catagagcag
     1921 gcaggtgtcc atgtgcgaga tggccagcgg cacttccagc atggccaggg ccgagacatg
     1981 cggggcatgc gagtggacga tgcactggat atcggcgcgg gcgcggtaca gccacgaatg
     2041 gaagcggttg gccgggttgg gcatgccctg accttcgagc acattgaggt cctggtcgac
     2101 cagcagcagg ttgtcggccg tgatctcgtc gaagcccagg ccaagctgct gggtccagta
     2161 ggtgccgggg cgctcggccc gcgccgtgat ctggcccgac agggccgagt cgtggccaaa
     2221 ggcgaacagg atgcggcagg tttccgcgac cgcttctttc cgcgtcatct tggcgccgtg
     2281 cagcgccgtt tccattttct cgagcgaggc ggcgacgatc tcgcccttgc caagatgcat
     2341 ggtatctgcc atgcggatct cctgaggttg gttcttatgg ggggagctgg atcagcttac
     2401 ttgccggcga ccgagggcgc caacagcctg gcccagcgct tgtcttcctg cgcgatcaag
     2461 gtcttgaagc cttgcggcgt ggaggattcg gcggccgtgc cctgggcctt cagcagcgcc
     2521 ttgaattccg gcttggccac ggcctgggtc agcgccgtgc tcaggatctt gaccacgtcg
     2581 tcgctcaggc cggcggggcc gaccacgccg gcggccgagc ccgacaggag ctcgggatgg
     2641 cccagttcgg ccgtcgaggg caggtcgggc gcgctgtcga tgcgcgaggt atgcgccacg
     2701 gcgatcgccc gcattttccg cgcattgacg aaaggcatga cgacgggcgt ggcctcgaag
     2761 gtcatgtcca cctggccgga cacgacgttg gtgaccgatt cgatgccgct cttgaacggc
     2821 acgtgcagga atttcacgcc ggtcgattct tccatgatga ccataccctg gtagggcgag
     2881 ctgccgatgc cggccgtgcc gaaggtgagg ccttcgggct tgcgcctggc cagggcgatc
     2941 agctcttcca ggttgtgcgc gggcaccgag gggttgacca cgatgacgtg cggggtcgat
     3001 gccaccagcg agaggtagct gaagtccttg ctgttgtcga aaggcgcgcc gtcggccgtg
     3061 tgggggttga cggccagcag gcccacgggg gcgaacagca gcgtgtagcc gtcgggccgg
     3121 gcgcgcttga cgtagtcggc cgcgatattg ccgcccgcgc cgggcttgtt ttcgatgatg
     3181 acggactggc cgacctgctc ggacagggca cggccggtgg cgcgggccac cagatcggag
     3241 atgccgcccg cgccgaaggg caccaccatc gtgatcgcat ggtcgggata gccggcggcg
     3301 tgggacaggg ccggcaatgc ggcgctgcag aacaggacgg attgcagcat tcggcgtagg
     3361 gcgtggttca ttgaaggcat ctccatcggg catgagtcgt gtcggaagcg cgccaaggtg
     3421 tccggcacgg cctgcgcgcc atgacacaat agcacttaat gacacaatgg tgtcatatcg
     3481 gaaacgaaac tgccaactgg aaaaccctag gaagtaaaat attcagatgg atgacaagga
     3541 aaatatcaac gtggtgggcg agcgcctggc cgtcttgcgc aaattgaatg cgctgaccct
     3601 ggaagaggtg gccaaccggg cgacgctgac caagagctat ctgtccaaac tcgagcgcgg
     3661 cttgtcatcg ccgaccatcg ccacggtgct caagctcgcc cgtgccctgg acgtcagcct
     3721 cgatcagctc atcggcggca cgtcgcccag ccgggagatc ctgcaccagc atgccgccga
     3781 ccgcgtgccg ttcagcccgt cgagcgcccg cgagggctat acctacgaag ccatcgccac
     3841 cgaccggccc gacaaggtca tgatgccctt catcatggcg ccgccgttta cgctggaaga
     3901 gcaggaattg atgagccatc ccggcgaaga gctcatctac gtggtttcgg gcgagatcga
     3961 ggtggtgttc gcggatcgca ccgtgcgcat ggcggcaggc gactcgctgt acttcaacgc
     4021 gtcgctggcg caccgctcgc gctcgctggg ggaggtgctg gcgcaggcgc tggtggtggt
     4081 cagcgatcct gcccggcgcc gctgaaacag tcagtcggcc agcttgcgca gaaaggcctc
     4141 gacggcccgg cctgcgagtt cggcatcgtc ggccgtgatg atttccagcg ggttgtggct
     4201 gatcccgcca ttgccgcagc gcgtgaacag catgctcacg ggggtgatct tctcgagcgt
     4261 catggcgtcg tggccggcgc ccgacaagag ttcgaagtcc ggcaggccca gcccggacag
     4321 cacgtcgcgc cacagggccc ggtgcggggc ggagcagggc gcggccggca ccgtcatggc
     4381 gggctgggtg tcctcgacgc tgacatggcg gcgcgtgcag atttcgtcga tcttgcggtt
     4441 gatgtcggcc atggccgcat cgcgcgtggc gtcgtcggcg gcgcggatgt ccaggctcat
     4501 gcggcaggcg cccgggatca cgttgaccga cccggagggc acttccagca tgccgacggt
     4561 gcccaccagg gtttcggcct ggccgcagcg cgcttccacg tagagcgcga tctcggccgc
     4621 ggcgcaggcg gcgtcgcggc gcatgtgcat gggggtggtg ccggcgtggc cggcgcggcc
     4681 cttgagcagc aggcggcggc gcacgctgcc cgcgatcgag gtgaccacgc ccagcgccag
     4741 gccggcgtcg agcagcaccg ggccctgttc gatgtgcact tcgaaataat gcttgatgag
     4801 cgccgggtcg atggcggcct gggcgatgcc ggccgggtcc aggccggcct cgtccatggc
     4861 ctggcgcagc gtgacgccct tggcatcggc caggtccagc caggcggggc tgtagcggcc
     4921 gatataggcc gacgaggcca ggaaggacgt gccgtagcgc gcgccttctt cctcggcaaa
     4981 gccgaccacc tcgaaatcgt agggcagccg ttcgccgcgc cggtgcaggt ccgccacgac
     5041 cgccatcggc agcaggatgc ccagccggcc gtcgtagcgg ccgccgttgc gcacggtgtc
     5101 gtagtggctg ccggtgacga ccagcccggg ggtggccacg gcggcatcgg ccttgtagcg
     5161 gccgaccaca ttgcccacgg catcgtggcg cacggtgtcg aagccggctt cccgcatcca
     5221 ttcgcccagc tgcctggccg cggcgcggtg cgcgggggtg aggtaggtgc aggtgaggtt
     5281 gccgtcctgt tcggaccagg ccgccagctt gtcggcccag gccatgacct gggcgccata
     5341 cagaagagtg tcgtcggatt gcattgcgag tgccgcggga tgaaagtcgg tcaggcgcta
     5401 tttaagccca atgcccgggg tttgtgtggg aaatggcccc aggattaccc cgaggccgcg
     5461 ggctcgcctg ccccgcggtt ttcggcggac gccgttctgc catgccgcgc cttgcgcggt
     5521 acggcgaggg tttgcggagt agtctggtaa ggtggtgtgt acggtatcga acagagtgcc
     5581 ggcctggctc ctggtatgtt ctttccggaa aaatatcgag ccgtcgaata cgacactctt
     5641 ttccctcgaa taccaaggat gtcccatgtc gaacatacaa gcaattcggg gcaatcactt
     5701 gctctcggtc ctgtccgagg tggagtgggc ccgcatcggc ccgcatctgg tgctggtgga
     5761 catgccgctg gggcaggtcg tctatgaatc gggcgaccgg ctcgaccatg tctatttccc
     5821 caccacgtcc attatttcct tgctctacgt gatggaagac ggcgcatccg gcgagattgc
     5881 catcgtcggc aacgagggcg tggtcggcat cgcccttttc atgggcggcg aaaccacgcc
     5941 cagccgcgcg gtcgtgcaga gcgcaggata cgcgtaccgc ttgaattcgc acatcctgaa
     6001 agaggagttc catcgcgcgg gcccgatgca gcggctgctg ctgcgctata cccaggcgct
     6061 catcacgcag atggcgcaga ccgcggtgtg caatcgccat cattccattg atcagcaact
     6121 atgccgctgg ctgctgctga gcatggaccg cctgccgtcg aacaagctga agatgaccca
     6181 ggagctgatc gccaacatgc tgggcgtgcg caggcctggc gttaccgagg cggcgatgaa
     6241 gctgcaggat gccggcctga tccgctacag ctacggcaat atcgaggtgc tggaccggcc
     6301 cgggctcgaa caccgcgtgt gcgagtgcta tgccgtggtc aagcgcgaat tcgaccggct
     6361 gctgcccgat ttgagagccc tgtaggccgg ccggcggaga tttccgattt gggtgcggca
     6421 ccgtaccgac cttggccgaa atgcggcgca gcctgttctc ctggggtgcc gccgcgcttg
     6481 cagcgcggcg tatcagcctg aatgcgttta acggggtgcg atatgaaaaa gcatggaatc
     6541 ctgctcttgc aatggatcgt ggtcttgagc gccacagtaa cggggggagc cgcgcttgcc
     6601 gccgatgaaa tgctgcccgc aacacagcat gcgggagcgg tgagctatgt gtccggcggg
     6661 atcggcctcg accagtcgaa agccctccag cgcgccatgc acaattaccc cttggtgctg
     6721 acgttcgtcc gcgcgaggaa aaatgatcgc gaatacctgg ccaacgtagc ggtaaccatc
     6781 aaggacgggc agggcaatac ggttctggac gccgcgtcgg acggtccgtt catgctggtc
     6841 tccctgccca atgggcgcta cacggtcagc gccagcttcc agggcaaatc gcagcagcat
     6901 gccgtggccg tttcccggtc ccagcacgtt cggcaaacgt tcagctggcc caggtagatg
     6961 ccggcatatg ggcattccct ctacctgctg ccattcgacc accgcaactc ctatgtaagc
     7021 gacctgttcc attttggatt tccgctcgat gcccggcagc aggcgcgggt cgaagacagc
     7081 aaacaggtca tttatgaggg gttcctggcc gccgtggcgc atgccgtgcc tgagcggtgc
     7141 gccggcatac tggtcgacga ggctttcggc gccgggattc tgcgcgatgc gcgcggccgc
     7201 ggctatgcga cggccgtatc ggtcgagcgc agcggctccg atgagttcca gttcgaatac
     7261 ggcgacgatt tcgcggcgca tatcgaagcg gtcgatccga ctttcgccaa agtgctggtt
     7321 cgctacaacc cggaagacga tgcggcgatg aatctgcggc aggccgcgcg gttgagggcg
     7381 ctcacgcagt attgccagag aacccaccgc ttgctgatgt tcgaattgct ggtgcccgca
     7441 accggcaggc agttgcaagg cgcgcatggc gaccgttcgg cctatgacct ggatgtccgg
     7501 cctgccttga tgctggccgc gatagccgcc ctgcaggatg ccggcgtgga gccggccatc
     7561 tggaagatcg agggcttgga ccgaagcgag gactgccgca gcatggtcgc ggcggcgcgc
     7621 cgcggcggcc gcggccatgt cggctgcatc gtgctggggc gcggggccga cgagaagaaa
     7681 gtgatcgatt ggctcgagac cgcggcgcaa gtcgacggct tcatcggctt tgccgtgggc
     7741 cggaccagtt tctatgatgc catcgccgat tacgtggccg gcaaggccac gcgcgaacag
     7801 gcggcaggcc gcatcgccgg gcaatacgcg aagtgggcgg cgatcttcga gcaggcgcgc
     7861 aaagccgggc cctgagcggc cggcttgcgg tctggcctat ggttccggca tcggcggggc
     7921 ggccgcggtg ggcgcggggt tgtgccagtg gccgtcgccg gtcatgatcc ggtcggcttg
     7981 ctcggggccc caggtctgcg gttcgtaggt gaccagcggc acgctgtccc ccagtatcgg
     8041 catcaccgtg cgccaggccg cttcgatgca gtcgtagcgg gcaaatagcg ccgcgttgcc
     8101 caggatcgca tcgtcgagca ggcgctcgta gggcgtcatt tcatcgccgg gatggcgatg
     8161 ggcgaccagt tcgacttcct ggcccagcat ggcctcgccg ggtatcttga cgcgcgtgcc
     8221 tatcgagatc agcacgtcgg ggctgatcct gaaccgaaag taattcaatt gctccgacgt
     8281 catcgcgtcg aagatctcgt ggggcggcgg cttgagcatg accaggatct cggtggaggt
     8341 cgcggccagg cgcttgccgg cgcgcaggta aaacggcacg cctgcccagc gctcgttgtc
     8401 gatatgcagg cgcagggcga tataggtttc tacgttggag cggggagcga catctttttc
     8461 gtcgagatat ccgcggaact ggccgcgcac ggcttcgccg ggggcaatcg gccgcatggc
     8521 tgcgaagacg cgcgatttcg catcgctcag cgcgtcgggg cggccatcgg ccggggcgtc
     8581 ggccgcgagc agcgaaacca cctgaagcaa gtggttctgc accacgtcgc gtatcgtgcc
     8641 cacgccctca tagaaactgc cgcgtccttg caccccgaac gattcggcca tggtgacctg
     8701 gacgctggcg acatgctccc ggttccagag cggttcgaga atcgcattcg cgaagcggaa
     8761 atagagcagg ttctggatcg gctccttgcc gaggtagtga tcgatgcgaa agatggccga
     8821 cgcgggaaac gactgcgtca gcagcagatt gagcgcttgc gccgaggcga ggtcgtgccc
     8881 gaacggcttt tcgacgatca cgcgggcgtc gtgcgcatag cccgactcgg ccaggccctt
     8941 ggccacgcgg ccgaacatcc cgggggggat ggccagatag aacagcgggt gcctcgctgg
     9001 tcccagtgcg gcgcgcagct gccggtaggt cgaactgtcc agcaggtcgc cgctgatgta
     9061 ctggagccgg ggggcaagcg tcttgaacac gtcgtcgtcc gggacgccat gcttgtcgat
     9121 gctgtcgcgg gcatgggcga tgaactgctc gcggcccaag gccgatctgc ccacgccaat
     9181 cacgggaata tcgaggcggc cgtgccgggt catggcctgc agcgcgggaa atatctgctt
     9241 gctggccagg tcgccggtgg cgccgaaaaa aacgaaggca tccgatttga aagggggcat
     9301 gatcaacggt cggaccgggt tgccggcttt tccagatggc cgccgaatga attccgcatg
     9361 gcggacagga tcttgtcggc gaagtccgca tcgccgcgcg aactgaagcg tgtgtagagc
     9421 gccgcgctca gcacgggcgc cgggacggcc tcgtcgatgg cggcgatgat cgtccagcgt
     9481 ccctcgccgg aatccgagac ccggcccgcg tactgcgtca gcagggggtc gtcccgcagc
     9541 gagccggcgg tgagatccaa taaccaggaa ccgatgacac tgccatggcg ccagacctcc
     9601 gcgatttccg gcaggtcgaa ctcatattga taatgttcag gcttgcgcag cggcgtggtt
     9661 tccgcatcgg cggcatcggc ccgcgagccg gcattggcgt tgcgcaagat attgaaaccc
     9721 tcggcatagg cggccatgag cccgtattcg atgccgttgt ggaccatctt gacgaaatgt
     9781 cccgcgccat gcggaccgca gtgcagatag cctcgctggg cgctgccggc gccggcggcc
     9841 ggttccggcg ccgcgggggc gtcggccgcg tcgggagcca gtgcggcaaa tatcggggca
     9901 agcgactgca cgacgctcgg ttccccgcct atcatcaggc aatagcctct ttcgagcccg
     9961 aagattccgc cgctcgtccc gacatccacg taatgaatcc ccttggcccg taattcggcg
    10021 ctgcgccgga tgtcgtcgtg gtaataggaa tttccgccat cgatgatgac gtcgccggcc
    10081 tcaagcaggg caagcaaggc cgccagttcc gcgtcgaccg cggcggcggg cagcatcagc
    10141 cagatggttc gaggtttttc caggcgcgaa acgaaatcct ggagcgtgtc gctaccggcc
    10201 gcgccgcgtt gccgcagtgt gttgacaagc tcggcatggg tgtcgtggac gacgcattcg
    10261 tggcccttct tcaacaggcg ccggaccatg ctgctgccca tgcgacccag tcctatcatg
    10321 ccgatttgca tggtgcttcc ttggaaattg gcggaggttc tcttgcccac ggtgcgcccg
    10381 gcgggggctg gattcagtcc gctaccgtac cgcggcccgg cgtgtacgct ggcagactga
    10441 ggtaagggca ttcctcactc agactgaaag cagtccggtg aatgatgcac aggcattcga
    10501 tggcgatggc cgataggcca tgccgggtcg ccgcggctgt tcacaaggag aacagaatga
    10561 ggaatattcg taccatcatc gtgactggaa tgatcgtgcc ggccatgctc gtgcttatcg
    10621 ccgggtgcgg cggcatgtcg cgtcggggac aagacaccgc ggtcggtgcc ggggtaggcg
    10681 gcgtggcggg agccgtcctg acgggcggga gcgtattggg cacggtcggc ggcgccgccg
    10741 ttggaggcgt cgtcggcaac caggtcggga gatgatcccg atgcttgcat ccgccgcgcg
    10801 cggggggacg catgcccggc cccgatgacg tcgtgcttct cttcgattgc gacaacacgc
    10861 tgtttgacaa cgacgcggtg ctggaagacc tgcgggcatg catggtccgg gaattcggaa
    10921 aaaccgatag cgatcggtac tggacgattt tcgaagccct gcgtctcgag ctgggctatg
    10981 tggactatct gggcgccctg cagcgctatc ggcgcaaaga ccagagcgat acgcgtgtga
    11041 tgcgtatgtc ttcattcctg atcgactatc ccttcgccag ccgggtgtac ccgggcgcgc
    11101 tggacgcgct gcggcgtctg cgcgccctgg gtaaaaccgt catcgtctcc gacggcgacg
    11161 tcgtcttcca gccgcacaag ctcaagcacg ccgggctgtg ggacgaggtg caggggcgcg
    11221 tgctgatcta tatccataaa gaaaccatgc tcgacgagat cgagaggtgc tatcccgccc
    11281 ggcgctacgt gatggtcgac gacaagctgc gcgtcttgtc ggcgatgaaa caggtatggg
    11341 ggaaccgcct gacgacggtc ttcccgcgcc aaggccacta tgcgctggat gccgcggcca
    11401 tcgcggctta tccgccggct gatgtcgtca tcgagcatat cggcgagctg gacgaagccg
    11461 tcctggcttt tcagccgggc gtcgacaggg ccggccgtat ttaacaagga gtttccatga
    11521 aaccgatatc ccagcgatcg ctgccgcttg gcgccgcggc cgtgtcgctt accgagcgtc
    11581 ccgcctggca ggcgctgatg gcgcattacc ggcagattcg caatctgcac ctgcgcgacc
    11641 tcttcgcgga agacccctcg cgcagcgagc gcatgacgct cgaaggggcc ggcctttatt
    11701 ttgattattc gaagcatcgg ataaccggcg agacgattgc gctcttgctg cggctggccc
    11761 gcgagtgcgg cctggcgcag cgtatcgacg cgatgttcag cggcgacgcg atcaatgcga
    11821 ccgagcagcg ggcggtgctt cacgtggccc tgcgcgcgcc ggccggcgaa cacatcctgg
    11881 tcgatgacgc cgacgttgtg cctggggtgc acgcggtgct cgaacgcatg gcggttttct
    11941 gcgaggacgt ccgcagcggc cgctggcgcg gccacaccgg ccggccgatc cgcaacgtca
    12001 tcaatatcgg cgtcggcggt tccggcctgg gttcggagat ggcttgcgaa gcgttgcgcc
    12061 actatagcca gcgcaatatg acattccgtt tcatctccaa cgtggacggc accgacttcg
    12121 tcgaaaacac gcgcgatctc gaccccgggg agaccttgtt catcatttgt tcgaaatcct
    12181 tcacgacgac cgagaccctg agcaatgcgc gccttgcccg cgagtggagt ttgcgcgccc
    12241 tgggtgacga gagcgcaata cgtcaccact ttgtcgccgt gtcgaccaat gcgcaaggcg
    12301 tggcggattt cggcatcgac atggcgtata tgttcggctt ttgggactgg gtcggcggcc
    12361 ggtattcgat ggactcggca ggagggctgt cgatcatgat gtccatcggg gccgagaact
    12421 ttcaggcgct gctggccggc ttccattcga tggaccagca tttccgcacg acgccgttcg
    12481 agcgcaacct acccgtcctg atgggtttgc ttgcggtctg gaacagcaat ttcttcggcg
    12541 cggagacggt tgcggtattg ccctacgaac aatatttgaa gcgtttcccc gcgtatctcc
    12601 agcagttggc gatggaaagc aatggtaagc atgtctcgtc cgacggaacg cgattcgctt
    12661 acggaaccgg tccgatctac tggggcgagc cgggaaccaa cggccagcat tcgttttacc
    12721 agctcctcca ccagggtacc catatcgtcc cgtgcgattt catcggcttc ggccgaagcc
    12781 tgaacccgct tggcgatacg catgacttcc tgatggcgaa tctcttcgcg cagaccgaag
    12841 cgctggcctt cggcaggacg gcggcgcaga tcgaagccga cggcacgccg tcctggctgg
    12901 cgccgaatct cgtctgcgaa ggcaatcgcc cctccagcac gattctggcc gaaagcctga
    12961 cgccggaaac gctgggacaa cttgttgcac tttacgagca cagcgttttc acgcaaggta
    13021 cgatctggaa tatcgactcg ttcgatcaat ggggtgtcga gttgggcaag aagcttgcgc
    13081 ggaaaatcct ccccgagctt cagggggcgg gcccggccga gctggatcac gatagttcaa
    13141 ccaatgcgct gatccggaag tatcgccatc tgcggacgcc gtcgccatga atccgaatga
    13201 tgcaggaggt agctcgatgc gtgtcagtcc cctcgccggg aaaccggctt cccgttcaat
    13261 actggtcaat gttcccaggc tgatcacggc gtattacagc gaccgcccgg atcccgcaat
    13321 accggaacaa cgggtggtat tcgggacgtc ggggcatcga ggctcgtcgc tggcttgcag
    13381 cttcaacgag tggcatgtgc tggcgatcac gcaggccatt tgcctgtatc gcgcgcagcg
    13441 gggcatagcg gggccgctgt tcatcggcat agacacgcat gcgctttccg agccggccta
    13501 cgcgagcgcg ctcgaggtgc tggccgccaa cgatgtggaa gtcatgattg cgcaaaatgg
    13561 cgaatacacg cccacgcccg ccgtgtcgca cgccatcatc gcctacaacc gcggccgcag
    13621 cgcaggcctg gccgacggga tcgtcgtgac gccttcgcac aacccgccgg aaagcggcgg
    13681 tttcaaatac aacccgccga acggtggtcc ggccgattcg atcgtgacgg gctggatcga
    13741 agcggcggcg aaccgtctgc tcgaaaacca gctcaagggg gtgcgccgca cgccgctatc
    13801 gaaggccatg cgcgcctcga ccacgcatcg gcatgatttt ctcaatgcct acgtgggcga
    13861 tctcgtccat gtcatcgaca tggacgtgat gcgcggcgca ccgatccatc tgggcgtcga
    13921 tccgctcggt ggcgcgggtg tgcactactg ggagccgatc gcggcgcgct acggcctgaa
    13981 cctcaccgta ctcagcgatg tggtcgatcc gaccttctgc ttcatgagcg tcgattggga
    14041 cgggcgcatc cgcatggatc cgtcgtcgtc ctacgccatg cagacgctga tcggccagcg
    14101 ggaccacttc gacgtggcgt tcgcgtgcga taccgatcac gaccgccatg gcatcgtcac
    14161 ccggtacggc gggctgctgc cgccgaatca ctatttgtcg gttctcgtcg attatctcta
    14221 tgcgcaccgg ccccgatggc gggcggatgc ggcagtgggc aagaccctgg tcagcagcca
    14281 gatgatcgat cgcgtggcgg aaaagcgcgg ccgcgcgctt tacgaagtgc cggtggggtt
    14341 caagtggttt gccgacggcc tgctcgatgg ttcgctgggt tatgcgggcg aggaaagcgc
    14401 gggcgcgagc tgcctgcggc tcgacggcac ggcctggacg accgacaagg acggcatggt
    14461 gcctgccttg ctggccgccg agatgacgat acggacgggc cgcgatcccg cggagcttta
    14521 ccaggatctg accgagcagc tgggcaagcc ggtcgagcgc cgtgtccagg cggcggccat
    14581 ggcgccgcag cgcgcgctgc tggcgcggct ttcgccggcg caattttccc ataccgagct
    14641 ggcggacgag aagatcgagc gggtgctcga ccacgcgccg ggcaatggcg cagccatcgg
    14701 cggcatcaag gtggccacga agaacggatg gttcgccgcg cggccttcgg gcacggaaga
    14761 tatctacaag atctatgcgg agagcttcct cggcgaggac cacttgacgc gcatcgtcga
    14821 cgaagcccag gcgatggtcg atagcgcgct gagccgggtg ccgctaccct agcagcgcca
    14881 aggctcgcgc gcaaacgttg tcgacggtaa atccgtattc gcgcagcagg acgtccgccg
    14941 gcgccgaggc gccgaaacgg tccatgccga gtatgccgcc atgttcgccg acatagcggt
    15001 gccagccctg cggcacgccg gcctcgatcg ccagccgcgc gctcaccgat ggcggcagca
    15061 cggcatcccg gtacgcttgc gcctgggcgt cgaacagggt ccagcagggc agcgagacca
    15121 cgcgcaccgc gatgtgctgc gccagcaagc gttcgcgcgc ggccatcgcc agcgcgactt
    15181 cggagccggt ggcgatcagg atcaatgccg gcttgccgcc gggtgcgtcc gcgagcacgt
    15241 agccgccgta ccgaagttcg tcggcagcgg cataccaggt gcggtcgagg gtcggcacgt
    15301 tctggcgtgt caggaccagc gcggtgggac gatcgttcgt ttccagcgca atgcgccagg
    15361 ccaccgccgt ttcgttggcg tcggcggggc gtatgacatt gagcccgggc accgaccgca
    15421 ggctcgcgag ctgttcgacg ggctggtgcg tcgagccgtc ttcgccgagc gccaggctgt
    15481 cgtgcgtgaa gataaaaatc acgtgcagat gcatgagcgc ggccagtcgg atcggcggac
    15541 gcatgtaatc ggaaaagatc aggaatgtcg cgccgaaagg gacgatgccg ccatgggcgg
    15601 ccaggccgtt gagcacggcg cccatggcat gttcgcgcac gccgaagtgc acgttgcggc
    15661 cggcgcggct ccagccgccg ccgcccgagc cttgccggtc gtgggcattg gcgccgggcg
    15721 cggcgaagtc gccaagttcg gacaaggcgg taaaggtcga cggatcgaga tccgccgagc
    15781 cgcctagcag tgttggcaga tagggggcaa tcgcgttcat gacctggccc gatgcgaccc
    15841 gtgtggcgat gccttcggca tccgcgggaa aaatcgggat gccacggtcc cagccgtcgg
    15901 gcagtccgcc gcgcatgact tgccgcaatt cttcggcaag cagggggaaa gcctgcgcat
    15961 aggaagagag cagggtgttc cacccggcct cctgctgcga gccctgcgcc agtgcgcggc
    16021 ggaaatgcgc cagggctgcg tcgggaatat ggaagtccgg ctcggttggc cagccgaggt
    16081 tctgcttggt caggcgcgtt tcttcggcgc ccagcggcga gccatgcgcc ttgtacgagt
    16141 cctgcttgtt gggcgaacca tagccgaggt gcgtgcgcac caggatcagg gatggacgtt
    16201 gcgtttcggc tcgggcgtga cccagcgcct gcccgatggc cgccagatcg ttgccgtcct
    16261 cgacacactg ggtatgccag ccgtaagcct cgaagcgccg ggcacggtct tcggtaaacg
    16321 tgatgtccgt gcccgcggaa agcgtgacct ggttgtcgtc gtacaggtag atcagcttgc
    16381 cgagctggag gtggccggcc agggaggccg cttccgacgc aacgccttcc atcaggtcgc
    16441 cgtcgctgac gatgccgtag gtgtggtgat cgacgagggc gaatcccgga cggttgtagc
    16501 gcgcggcgag gtaggcttcg gcaatggcca tgccgacgcc gttggccatt ccctggccaa
    16561 gcggtccggt cgttgtttcc acgcccggcg tcaggccgcg ttcgggatgc cccggcgtgc
    16621 ggctgcccca ctggcggaac cgcttgatct cttcgagcgg cagatcgtag ccagtcaagt
    16681 gcagcaggct gtacagcagc atggagccgt gcccggccga cagcacgaac cggtcgcgat
    16741 cgaaccaggc cggattggcc gggttgtgct tgagaaagcg ggaccatagc gcgtaagcca
    16801 tgggcgcggc gccgagcggc aggccaggat ggccgctatt ggctttttgt accgcgtcga
    16861 tggccagaaa gcgcaaggtg ttgacgcaca gcgcatccag tgtcggagtg tccatagggt
    16921 ccgctacagc acgagcttgc tgaccttggt gccttcaagc gaaacccccg ccatcaatcc
    16981 gctgttggtc agcaggaagg cattgacctg cccgaccgcg gtgctggtgt cgacagcgcc
    17041 attcgctccg actttcaaga cggccaccga tgcgtcgccg ccagccgacc agccgtcggc
    17101 cttgcggaac tggttgagcg attcatcggt catgaacagg aagatgatcg cgcgggattg
    17161 cgcgccgatc tgccatccaa tcgagccggt taccgtgctg tagtacccgg cagtgcggtc
    17221 gctgatgcgc agcgcgcctt cgccgtactg gccgccgacg ataaaacccg catcgatgac
    17281 cgacggaaat accagcacgc catgggcctt gttgacgagt tcatgggaac ccttggccgc
    17341 ggcataaagg cgagtcagcg tgtcgtcgac accagcgttg atcgtacggc gcttgtcggt
    17401 ctgctgccct tgcgttttga catcggaggc gccggtggtg gtgcagccgg cgatggcgaa
    17461 gccgaccgca agcaccgatc cgggcgcgct ccacatgaat tttcgtctta gcattatctt
    17521 ctcctggttc attgatgacg tgttcgacgc tacatgagtc cgtgcgtcca tgctgtacga
    17581 aagcgtacag agcgccctgt ttctcatccg ccgtggcgat cacgggcggg gcctgtccac
    17641 tgctggcctg cctgcgaagc tagcgcagcg gcaggaacag attgatgcca ggcgcggctt
    17701 gcggcccgta gatgatgggc ggcgcgccgt agacatagcc gggatagcct tgatagtagt
    17761 tgccccggcg gcggttatcc tgtcgatgta tctgccggac gggctgcctc tggacatgcc
    17821 gctggccgcg attctggccg tgattctgcc cgccttggcg atggccttgc gcgtgcagcg
    17881 gcggcgttgc caacaggcag gccagtgcga tggccaggcc ggccgcggcc ggccatttct
    17941 tcagtatcga atctctcgct ggagataatg cattcgagag cttcatgatc agcccttctt
    18001 tttcatcatg tgattttcat cgcgaaaaat caggtcggca ttgcgcttct gggcgtccga
    18061 catgctggcg tagagcgctt cgaagggcgt ttcgagtttc ttgatgccgt ccgcatgcgc
    18121 gtcgattgcc tcgccatagg ctcgaagatt gtccacggcg ttcatattgc tcttgtcggc
    18181 ggtgcgggac tgtctgagcg catccaatgc ggcggcattg tcgcgcatga cctgcgcaat
    18241 ggactgccag aggggttcct gggtggccgt gatctgcagg cgcgagtgaa gactgttgat
    18301 gcgtgtatcg accgaactgg cggccgtgac gggcatggcg tgcgtcgtgg gcgatgccgc
    18361 gtggacggga ttggacagcg ctgccgccgt gccgagaagc agaatgactg cgaatgcgat
    18421 ggcggttcgc ggccaggggg taaggagagt gcgttgcatg gtgcgcctcc ttagcgggtc
    18481 gggacgatga gcaccgcgcc gtcggtggag gtcgcgccgc gcgcgccgtt gttgcctctt
    18541 gctcctgtgt tgccggtcgc gcccgtatcg ccggtcgcgc ctgtatcacc ggtcgctcca
    18601 gttgcgccgg tcgcgccgct gtagcccgag tccccccgct gtccggttct tccggtatag
    18661 ccggtattgc cggtattgcc ttgaggtccc ggcgggcctg tgcaggcgcc caatgcgagc
    18721 gtagccaggg tggcggcaat cagtttcgaa tattccatga tgactccttt gttttgtctg
    18781 catgaatgat gtagacacta cgggaacatc gcagcctatt cggtacgcta ccgagcatag
    18841 acctgaaaga attcaaaaat aggcaagcag gcaagacggc catcccattc gtgacggccg
    18901 cgagtcagct ggatttccct tttgttgcaa tgactctcat caggttgttc caggatcggg
    18961 tgaacgattg ccggccttca ttctggagct gggcggcaag cgccacgatg tccacgccca
    19021 ccagcgcgaa ttcggcaaga atggcttccg cgttgccgcc gtcttcccgc attgcgccgt
    19081 tcacttcccc ttgttccgcg aactggcgca gggttttctc cggcatggtg ttgatcgtgt
    19141 gcggagcggc cagcgcctcg acatagtatc cgccaggcaa tgccggatct ttcgtcccgg
    19201 tgctggccca caagaggcgt tggggcaggg cgccatgatc ggcgagcttg cgccactgcg
    19261 gcgtagccag ccactcccga tgggccttgt atgtctgctt cgcaatggcg atgcccagga
    19321 gattgtgcaa cgccgaggga acgctggcct gcacggcctt gtcccagcgg ctgatgaaga
    19381 tcgatgccac gcagtggact ttcggatcga gatcggcatc tatccggcgc tggatgccgc
    19441 gcgcataggc atcggccgcg gccagatagt ggtcttggga aaacagcagt gtgacattga
    19501 tcggcacgcc ggcgaagatc agttcttcga cggccaggat tcccgccgtc gtgccgggaa
    19561 ccttgatgaa caggttgtcg cattgcgcgc tggcatggag ttgcctggct ttttcgatcg
    19621 tgccggctgt gtcgtcggcc agcagcggag acacttccag cgaaacccag ccgtcaacgc
    19681 catggcttgc ctcatggatg aggcgaaaca gcgtggcggc tcgcgtaagg tcatcgatcg
    19741 ccaggtcgaa gaacagatcc tcgagcgatt ttccttcccg gattttcatg tcgatgtctt
    19801 tgtcgtaggc gctggtgttt cgtatggcct cgtcaaagag cgtcgggttg gatgtcaggc
    19861 cggtgacgca tagttcgtcg cggtagtgct cgagcgtgcg gctggtcagc agatcgcgcg
    19921 tcatgttgtc gagccacagg ctttggccaa gggcgtgaag ttgcgttgtt gccttcatgg
    19981 gagacctcgc tggcgcgtcg gggagaagaa cctgaattcc gattcaccat gcacgcaatc
    20041 gcgcctaaaa gcaagccgga tattgcgtac ggcagcgtac agactcgggc atgaaaagtg
    20101 gccaccgtgt attggatgtc ttgagttttg atcgttcatt gaagaaggtt ggccactatg
    20161 aatcagattc ccttatttct atcccttgtt ttcattgtgg cgggcgctgc ttcaagtcag
    20221 tggatcaacg tttatctggg tcctgtcctg atcgtcatcg gggttctcat cggcctgtca
    20281 tccaaggtgg ccaatgtctg ggagaagttc gtgatcctca ggctcggcaa gctgcagagc
    20341 gtcaagggag ccggcttctt catgatcatt cccgtcctgg atcgcatcgt ggcgatcatc
    20401 gatgaacgga tccagaccac ggcattcaac gcggagcagg cgctgaccaa ggatacggtg
    20461 ccggtcaatg tcgacgcgat tattttctgg catgtgagcg atgcgcagaa agcggcgctt
    20521 tccattaccg actaccgcca agcgatcgat cgcgtatcgc agacctcctt gcgtgaattg
    20581 atcgggtctt caatgctgtc catgctgctt tcggatcgcg cggcggcgga ctcgcgtctt
    20641 cggaccgaga tagaaggcaa gacctccgcg tggggcatcg cggtcagttc cgtggagata
    20701 cgggacatcg cgattccggt cgcgctgcag gatgcgatgt cccgccaggc gcaggccgag
    20761 cgcgaaaagc aggcccgcgt catcctgggt tcggcggaag cggcgattgc cgcgaatttc
    20821 gtcgaggcgg cgaggattta tgaaaaccag cccggcgcgc tgcaattgcg cgcgatgaac
    20881 atcatttatg aaacgaccaa ggagcggggc gcaaccatcc tc
//
LOCUS       DASWPO010000357         6260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_173374, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000357 DASWPO010000000
VERSION     DASWPO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173374"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VGC69_19135"
     CDS             complement(<1..737)
                     /locus_tag="VGC69_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297448.1"
                     /translation="MAADAMSGKPRVLSVEGLSIALPKGADRELAVRDVSLEIFKGET
                     VCLIGESGSGKSMVANAVMGLLPQPYVRPVAGRIDFEGRNLLAMTDRGLRKLRGEKMA
                     MVFQDPLSALNPLLRIGTQLDEMLRAHRSGMPAEERREKIVQALREVELPNPGLLGQS
                     YPFRLSGGQRQRVLIAAALLLEPDLLIADEPTTALDVTTQAAILALIHRLQQKTDTAV
                     LFITHDFGVVSEIADRVAVMRHGEIVER"
     gene            complement(739..1578)
                     /locus_tag="VGC69_19140"
     CDS             complement(739..1578)
                     /locus_tag="VGC69_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297449.1"
                     /translation="MKQTFWRRFCSNRGALIGLVILTIVVLVAVFAGVLAPNDPWDMV
                     QRPFLPPFIDPNLPLGSDVLGRDVYSGLIMGARVSLLIGVSAALAGLLIGITVGALAG
                     YFGGMVDTALMKLTELFQAIPHIVLAIVLVAIFHPSVVSIVVAISVVSWPSVARLVRG
                     EFLSLKERDYVHASILAGQSRLQIIVFQILPNAAGPIVVMGSLMVATAILTESALSFL
                     GLSDPNVMTWGYMIGAGKTALRTAWWAVALPGLAILLTVLAINLVGEGLSDGLNTRIR
                     VGG"
     gene            complement(1655..2629)
                     /locus_tag="VGC69_19145"
     CDS             complement(1655..2629)
                     /locus_tag="VGC69_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297450.1"
                     /translation="MSRLLFFVKRVLQGIVVILLIAVINFFLVRAAPGDPVSVLAGEA
                     GASDPMFVEQLTRHFGLDKPLPEQLATYMLHVVQLDLGQSYRQGQPVLTLIVDRLPAT
                     LLLTVTALIVSLIVGVFLGAASSMRRGSLVDTLISLLSLVFYAMPLFWLGLMLVLLFS
                     VHLDWLPGFGYETVGGAATGWARAIDIGRHLVLPVVTLASFYVAIYARMTRTSMLEVN
                     QMDFVKTARAKGLSPMRILTHHVMRNALLPVLTLAGIQAGAIVGGTVLVETVFAWPGI
                     GRLMFDSLMQRDYNLLLGSFLFTAALAVFFNILTDLAYTIVDPRIQLK"
     gene            complement(2681..4246)
                     /locus_tag="VGC69_19150"
     CDS             complement(2681..4246)
                     /locus_tag="VGC69_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297451.1"
                     /translation="MKRRDFMRAGAYGASAFFLDLTLPQWARAAQGGVLNIVVQPEPP
                     GLMLGMVQNGPTQLVSGNIFEGLLRYDEKLNPMPGLATKWEQAADGLSYTFTLKQGVT
                     WHDGKPFTAQDVVFSATKFLPEVHARFRANMTHVDSVTAPDDYTVVFKLKAPVGPFLG
                     IFEVGSMPITPRHLYEGTDYRNNPANNAPIGTGAFAFKEWKRGEYIHLVKNPGYHEPG
                     LPHVDEVYFQIIPDAASRAAAFETGKIDILPGGSVEYFDVPRLSKLPKVQVTSKGWEF
                     FAPHALMWVNNKNPALADKRVRKAILHALNRQAMVDVVLQGYGKVALGPFNSAIKFFS
                     DKVEKYPYDPKKAQDLLKAAGYKGEKLVMLPLPYGETWQRIAEMAKQNLVKAGFNIDL
                     QALDIATRNKRVESRDFDLTFTYLYEYGDPELGVARNYVSSSIANGSLYNNVASYSNP
                     EVDKLFAAGAVASDPAKRGEIYAQVQKILVDDAPVAWLFEMFFPTIYHDRVSNVVNSG
                     IGVNDGLARTQFV"
     gene            complement(4284..5510)
                     /locus_tag="VGC69_19155"
     CDS             complement(4284..5510)
                     /locus_tag="VGC69_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0297452.1"
                     /translation="MAVSNTGPDAASGPLPAAFRHLLLTRMGSSFAYQIVSVALGWQV
                     YAMTGNPLDLGLIGLAQFLPMLVLTLFVGQAADRYNRRAIVAICLTVEACAALALGAS
                     ALTGTGGRGLLYGLVVLMSIARAFESPTLPTLLPALVPRASIARGTAMFTSANQAAQI
                     LGPALGGVGYGLGAGWVYCVAALLFLLAMLSIVTVAVPHQARRNEVVTLGTFFAGIKF
                     IFQRRILLGTLSLDLFSVLLGGATALLPIYAKDILHAGPWALGLLRASPACGAILMSL
                     ALARHSLRGHIGRQLFSALLVFGLATVVFGLSRSIPLSIFALATIGAADSLSMVVRGT
                     LVQLNTPNEMLGRVSAVNMLFVGTSNQLGEFESGLTAALLGAVPAVIVGGVGTIVVTL
                     LWMWWFPELRRLRSLQ"
     gene            complement(5554..>6260)
                     /locus_tag="VGC69_19160"
     CDS             complement(5554..>6260)
                     /locus_tag="VGC69_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0297453.1"
                     /translation="TGLIERRRATLLPPPAEAPADVLALACAAVLAGQSLPASQVPAA
                     PADPWDARDGWRLGGGAYVQTIEWLANAAARCATIGHKGAHWRIDCGAGLQDYHWTTQ
                     AAAAGAAGLAVRVVLDGRDLGGAVVAQGEQVHVFMDGNTYVLERHDAMAHAGDDEQGH
                     AGGLAAPMPGKIIAISVKAGDTVRKGQALLVMEAMKMEHTIAAPADGTVREVFYAVGD
                     QVTEGAELAAIDAADK"
BASE COUNT         1109 a         2083 c         2137 g          931 t
ORIGIN      
        1 cgctcgacga tctcgccatg ccgcatcacc gccacccggt cggcgatctc cgacaccacg
       61 ccgaaatcat gcgtgatgaa gagcactgcc gtgtcggtct tttgctgcag gcgatggatg
      121 agcgccagga tggccgcctg cgtggtcacg tccagggccg tggtcggctc gtcggcgatc
      181 agcaggtcgg gctcgagcag cagggcggcc gcgatcagca cgcgctggcg ctgcccgccc
      241 gacaggcgga acgggtagct ctggcccagc agcccggggt tgggcagctc cacttcgcgc
      301 agggcctgca cgatcttctc gcggcgctcc tcggccggca tgccggaccg gtgcgcgcgc
      361 agcatctcgt ccagctgtgt gccgatgcgc agcagcgggt tcagcgccga cagcgggtcc
      421 tggaagacca tggccatttt ctcgccgcgc agcttgcgca ggccgcggtc ggtcatggcc
      481 agcaggttcc tgccctcgaa atcgatgcgc ccggccaccg gccgcacata gggctgcggc
      541 aagaggccca tgaccgcatt ggccaccatg gacttgcccg acccgctctc gccgatcagg
      601 cagacggttt cgcccttgaa gatctccagg ctcacgtccc tgaccgccag ctcgcggtcg
      661 gcgcccttgg gcagggcgat gctcaggccc tcgacggaca ggacgcgcgg cttgccgctc
      721 attgcatcgg ccgccatgtc agccgcccac ccggatgcgc gtgttcaggc cgtcgctcag
      781 gccttcgccc accaggttga tggccagcac cgtcagcagg atggccagcc ccggcagcgc
      841 cacggcccac caggccgtgc gcagcgccgt cttgccggcg ccgatcatgt agccccaggt
      901 catcacgttg ggatcggaga ggcccaggaa gctcagcgcc gattcggtga ggatggccgt
      961 ggccaccatc agcgaaccca tcaccacgat ggggccggcc gcgttgggca ggatctggaa
     1021 gacgatgatc tgcagccggc tctggcccgc caggatggaa gcgtgcacat agtcgcgctc
     1081 tttgagcgac aggaattcgc cgcgcaccag ccgcgccacc gacggccacg acaccaccga
     1141 gatcgccacc acgatggaca ccaccgacgg gtggaagatc gccaccagca cgatggccag
     1201 cacgatgtgc ggaatggcct ggaacagctc ggtgagcttc atcagcgccg tgtcgaccat
     1261 gccgccgaaa tagccggcca gcgcccccac cgtgatgccg atgagcagcc ccgccagcgc
     1321 ggccgacacg ccgatcagga gcgacacgcg cgcgcccatg atcagccccg aatacacatc
     1381 gcggcccagc acgtcggaac ccagcggcag gttggggtcg atgaagggcg gcaggaaggg
     1441 gcgctgcacc atgtcccagg gatcgttggg cgccagcacg ccggcgaaca cggccaccag
     1501 caccacgatg gtcaggatga ccaggccgat cagggcgccg cggttgctgc agaatctacg
     1561 ccagaaggtt tgtttcattt cagcctccgc ccggccgccc gaaggaggct gaagccccct
     1621 cggggggcag cgaaggtagt gagcgtgggg gtcgtcattt cagttggatg cgcggatcga
     1681 cgatggtgta ggccaggtcg gtcaggatat tgaagaacac ggccagggcg gcggtgaaga
     1741 gaaagctgcc cagcagcagg ttgtagtcgc gctgcatgag ggaatcgaac atcaggcggc
     1801 cgatgccggg ccaggcgaag acggtctcca ccagcaccgt gccgcccacg atggcgccgg
     1861 cctggatgcc cgccagggtg agcaccggca agagcgcgtt gcgcatcaca tgatgcgtga
     1921 gaatgcgcat cggcgacagg cccttggcgc gcgccgtctt gacgaagtcc atctggttga
     1981 cctccagcat ggaggtgcgc gtcatgcgcg catagatggc cacgtagaac gaggccaggg
     2041 tgaccaccgg cagcaccagg tgccggccga tatcgatcgc ccgggcccag cccgttgccg
     2101 ccccgcccac ggtctcgtag ccgaagccgg gcagccagtc caggtgcacc gagaacagca
     2161 gcaccagcat caggcccagc cagaagagcg gcatggcata gaacaccagc gacagcaggc
     2221 tgatgagcgt gtccaccagc gacccgcgcc gcatgctgga cgcggctccc aggaacaccc
     2281 ccacgatgag cgacacgatg agcgcggtca ccgtcagcag cagggtggcc ggcaggcggt
     2341 cgacgatcag ggtcagcacg ggctggccct gccggtacga ctggcccagg tcgagctgca
     2401 ccacatggag catgtaggtg gccaattgct cgggcagggg cttgtccagc ccgaagtgcc
     2461 gggtgagctg ttcgacgaac atcgggtcgg aggccccggc ctcgccggcc agcaccgaga
     2521 ccgggtcgcc cggcgcggcg cgcaccagga aaaaattgat caccgcgatc agcaggatga
     2581 ccacgatgcc ctgcagcacg cgcttgacga agaaaagtag acggctcatg gatgcttctg
     2641 aatggcggac ggccctgtgt acgggccgtc cgctctgggc ctagacgaac tgggtgcggg
     2701 ccaggccgtc gttgacgccg atgcccgagt tcaccacatt gctcacgcgg tcatggtaaa
     2761 tggtggggaa gaacatctcg aacagccagg ccaccggcgc gtcgtcgacg aggatcttct
     2821 gcacctgcgc gtagatctcg ccgcgcttgg ccgggtcgct tgccaccgcg ccggcggcaa
     2881 agagcttgtc gacctcgggg ttgctgtagc tcgccacgtt gttgtagagc gagccattgg
     2941 cgatggacga gctgacgtag ttgcgcgcca cgccgagctc gggatcgcca tactcgtaca
     3001 agtaggtgaa ggtgaggtcg aagtcgcgcg actccacgcg cttgttgcgc gtggcgatgt
     3061 ccagcgcctg caggtcgata ttgaagcccg ccttgacgag gttctgcttg gccatctcgg
     3121 cgatgcgctg ccaggtctcc ccatagggca gcggcagcat caccagcttc tcgcccttgt
     3181 agccggctgc tttcagcagg tcctgggcct ttttcgggtc gtagggatac ttctcgacct
     3241 tgtcggaaaa gaacttgatg gccgagttga agggccccag cgccaccttg ccgtagccct
     3301 gcagcaccac gtcgaccatg gcctggcggt tgagcgcatg caggatggcc ttgcgcaccc
     3361 gcttgtcggc cagcgccggg ttcttgttgt tgacccacat gagcgcgtgc ggcgcgaaga
     3421 actcccagcc cttggaagtg acctgcacct tgggcagctt gctcaggcgc ggcacgtcga
     3481 aatactcgac gctgccgccg ggcaggatgt cgatcttgcc ggtttcgaag gcggcggcgc
     3541 gcgaggcggc gtcggggatg atctggaaat acacctcgtc cacgtgcggc aggcccggct
     3601 cgtggtagcc ggggttcttg accaggtgga tgtactcgcc gcgcttccat tccttgaacg
     3661 cgaaggcgcc ggtgccgatg ggcgcgttgt tcgcggggtt gttgcggtag tcggtgcctt
     3721 cgtacaggtg gcgcggcgtg atgggcatgg agcccacctc gaagatgccc agaaaggggc
     3781 cgaccggcgc cttgagcttg aagaccacgg tgtagtcgtc gggcgcggtc acgctgtcga
     3841 cgtgggtcat gttggcgcga aagcgcgcgt ggacctcggg caggaacttg gtggcggaga
     3901 agaccacgtc ctgcgcggtg aagggcttgc cgtcatgcca ggtgacgccc tgcttgaggg
     3961 tgaaggtgta gctgagcccg tcggcggcct gttcccactt ggtggccagg ccaggcatgg
     4021 gattgagctt ttcgtcgtag cgcagcaggc cttcgaagat attgcccgac accagctggg
     4081 tggggccgtt ctgcaccatg cccagcatca ggcccggcgg ctcgggctgc accacgatat
     4141 tgagcacgcc gccctgcgcg gcgcgcgccc attgcggcaa agtcaaatcc agaaaaaacg
     4201 cgctggcacc ataagcacca gcgcgcatga aatcgcgtcg tttcacaatt accccttgtt
     4261 gtgcttcaaa ctcctcccga aagctattgc aatgagcgta gtctgcgcaa ctcgggaaac
     4321 caccacatcc acaggagagt gaccacgatc gtgcccacac cgcccacgat cacggccggc
     4381 accgcgccca gcaaggcggc tgtcaggccg gactcgaatt cgcccagttg attggacgtg
     4441 ccgacgaaca gcatattgac ggcgctgacc cggcccagca tctcgttggg cgtattgagc
     4501 tgcaccaggg tgccgcgcac caccatgctc aacgagtccg cggccccgat cgtggccaat
     4561 gcaaagatgg ataaaggaat cgacctcgac aggccgaaga ccacggtggc caggccgaac
     4621 accagcaggg cggagaacag ctgccggccg atatggccgc gcaggctgtg gcgcgccagc
     4681 gccagggaca tcaggatggc gccgcaggcc ggcgaggcgc gcagcaggcc cagggcccag
     4741 ggcccggcgt gcaggatgtc cttggcgtag atcggcaaga gcgccgtcgc accgcccagc
     4801 aatacggaga acaggtccag cgacaaggtg cccagcaaaa tgcggcgctg gaagatgaac
     4861 ttgatgcccg cgaagaacgt gcccagcgtg acgacttcgt tgcgccgcgc ctggtgcggc
     4921 acggccacgg tgacgatgct cagcatggcc agcaggaaca gcagcgcggc cacgcaatac
     4981 acccagcccg cgcccaggcc atagcccacg ccgcccagcg ccggcccgag gatctgcgcg
     5041 gcctggttgg cggacgtgaa catggccgtg ccgcgcgcga tgctcgcgcg cggcaccagg
     5101 gcgggcagca gggtgggcag ggtgggggat tcgaaggccc gcgcgatgct catcaggacc
     5161 accaggccgt acagcagccc ccgcccgccc gtgccggtca gggccgatgc gcccagcgcc
     5221 agcgcggcgc aggcttccac ggtcaggcaa atggccacga tggcgcggcg gttgtagcga
     5281 tcggccgcct ggcccacgaa aagcgtcagc accagcatgg gcaggaactg cgccaggccg
     5341 atcaggccca ggtccagcgg gttgcccgtc atggcgtaga cctgccagcc cagggcgacg
     5401 gagacgatct gataggcgaa cgaagaaccc atgcgggtca gcaaaagatg ccggaaagcc
     5461 gcgggaagcg ggcccgatgc ggcatcgggc ccggtattgg aaacagccat gaggcaaccc
     5521 tgataaggcg cgctcagcgc cgagggcctt attctactta tcggcggcgt cgatggcggc
     5581 cagctcggcg ccttcggtca cctggtcgcc cacggcgtag aacacttcac gcacggtgcc
     5641 gtcggccggc gcggcgatgg tgtgctccat tttcatggcc tccatcacca gcagggcctg
     5701 gcccttcctg accgtgtcgc cggccttgac cgaaatggcg atgatcttgc cgggcatggg
     5761 cgcggccagc ccgccggcat ggccctgctc gtcgtcgccc gcgtgggcca tggcgtcgtg
     5821 gcgctcgagc acataggtat tgccgtccat gaagacatgc acctgctcgc cctgcgcgac
     5881 cacggcgccg cccaggtcgc ggccgtccag caccacgcgc acggccaggc cggccgcgcc
     5941 ggcggcggcg gcctgcgtcg tccagtggta gtcctgcagc cccgcgccgc aatcgatccg
     6001 ccagtgcgcg cccttgtgcc cgatcgtggc gcagcgcgcg gcggcgttcg ccagccattc
     6061 gatcgtctgc acgtaggcgc cgccgcccag ccgccagccg tcgcgggcgt cccacgggtc
     6121 ggcgggggcc gccgggacct gcgaggccgg caggctctgg cccgccagca cggcggcgca
     6181 agccagggcc agcacatcgg cgggagcctc ggcgggcggc ggcagcagcg tggcgcggcg
     6241 gcgttcgatc agcccggtgt
//
LOCUS       DASWPO010000358         6772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_264760, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000358 DASWPO010000000
VERSION     DASWPO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264760"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VGC69_19165"
     CDS             <1..413
                     /locus_tag="VGC69_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0297454.1"
                     /translation="LPETMIERDGAFYVRSVMGGRPGGLAHFAPEALAEYERCAGLPG
                     WATGVCEDYRASATLDLEHDRAGRAAGERLRQPLRVLWGARGAVGANFDVPGLWSAVA
                     DTVSGQALPAAHYLPEEVPDLVLREAERFFGAAD"
     gene            499..717
                     /locus_tag="VGC69_19170"
     CDS             499..717
                     /locus_tag="VGC69_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY0297455.1"
                     /translation="MSDDSSPADAAPGGVFTMSDSPCVAICSTLFDDVCRGCGRTAEE
                     VSNWVFLSEEEKRAVWVRIRAQGYPRRP"
     gene            799..1845
                     /locus_tag="VGC69_19175"
     CDS             799..1845
                     /locus_tag="VGC69_19175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0297456.1"
                     /translation="MLIALVTDAWRPQVNGVVRTWTTMQQILTSWGHQLVVVSPEGGR
                     TVPAPSEPDLRLCLQPGKQLRRLLGDTVPDALHIATEGPLGLAARRMALRRGWRFTTS
                     FHTMFPEYLEARMGIPASLPWRYMRWFHRPSQRVLVPTPTVRGIVASRGIERSIVWAR
                     GVDTQKFQPEARDGLADLPRPIYLSVGRVAREKNLDAFLGLDLPGSKVVVGGGPDEAR
                     LKRKFPGVHFMGARPDTELPKFYSAADVFVFPSLTDTFGLVMFEAMACGTPVAAFRNE
                     APMAVVKPGLTGYLDDDLARACRDALGLDRGAVREYALTRTWDAIAHDLLAALVPLVE
                     GTEFSASDLAAARS"
     regulatory      1888..2013
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            2118..3521
                     /gene="ahcY"
                     /locus_tag="VGC69_19180"
     CDS             2118..3521
                     /gene="ahcY"
                     /locus_tag="VGC69_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0297457.1"
                     /translation="MTTKATDYIVADMSLAGWGRKELSIAETEMPGLMAIREEFGARQ
                     PLKGARIAGSLHMTIQTGVLIETLVALGADVRWASCNIFSTQDHAAAAIAATGTPVFA
                     VKGETLEQYWEYTHKIFEWPGEQANMILDDGGDATLLLHLGTKAETDISVLAKPTSEE
                     ERVLFAAIKKTLARDPKWYSTRLGQIKGVTEETTTGVHRLYQMSQKGELAFAGINVND
                     SVTKSKFDNLYGCRESLVDGIKRATDVMVAGKVAVVAGFGDVGKGCAQALAALRAQVW
                     VTEIDPICALQASMEGYKVVTMDEAAAHADIFVTATGNYHVITRKHMDAMKDQAIVCN
                     IGHFDNEIDVAGIEDLQWEEIKPQVDHVIFPDGKRIILLAQGRLVNLGCATGHPSFVM
                     SSSFANQTIAQIELFTRNESYTKGQVYVLPKHLDEKVAMLHLKKLGAKLTTLSKEQAD
                     YIGVPVQGPFKADHYRY"
     gene            3707..4042
                     /locus_tag="VGC69_19185"
     CDS             3707..4042
                     /locus_tag="VGC69_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY0297458.1"
                     /translation="MSLILVWILNAVALLVVAYLLPGITVASFGSALIAALVLGLLNM
                     LVKPVLVLLTLPITIVTLGLFLIVLNALLFWLAGSILRGFQVSGFWWAAGGAILYSII
                     SGLLSRIIP"
     gene            4052..4894
                     /gene="metF"
                     /locus_tag="VGC69_19190"
     CDS             4052..4894
                     /gene="metF"
                     /locus_tag="VGC69_19190"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY0297459.1"
                     /translation="MTATTQAYSLEFFPPRDPAAHERLVETARQLLVVQPKYVSVTFG
                     AGGSTQAGTSEAVRTLRGMGCDAAPHLSCMGATRARLREILQAYQAEGVKRLVALRGD
                     LPSGMGGDSGELRHARDLVAFIRAETGYWFHIEVAAYREMHPQAHNASTDLAHFADKV
                     KAGASSAITQYFFNADAYFDFVERARSLGVDVPIVPGIMPITNSSQLLRFSDMCGAEV
                     PRWIRLRLAEFGDDKASIRAFGHEVVTGLCERLLQGGAPGLHFYTLNQAESTLGVWRA
                     LTGR"
     gene            complement(4954..5817)
                     /locus_tag="VGC69_19195"
     CDS             complement(4954..5817)
                     /locus_tag="VGC69_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEY0297460.1"
                     /translation="MTYCVAVRLNSGLVFLSDSRTNAGVDQISVFRKLKVFEQPGERM
                     MVLMTAGNLALSQAVVNLLRQHDASHPDSLWQAPNMAEAARRIGEAVREVHRRDAASL
                     AEQGVEFNVNIIFGGQIGNEPCRLFQVYAAGNFIEASNECPYIQIGEAKYGKPILDRV
                     LAPGTTLDEAAKCMLISMDSTLRSNISVGLPLDLLVYETGALRVTHFACIDEDNEYFR
                     MIRTTWSNRLRQVFGEIPDPAWTAAAAVDALQPMRRSQQPVRVESAAIGAEQYLPLQV
                     LAQSNGDKQAS"
     gene            complement(5865..6632)
                     /locus_tag="VGC69_19200"
     CDS             complement(5865..6632)
                     /locus_tag="VGC69_19200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0297461.1"
                     /translation="MSRSGPSPDAIADSAPDLFESENAALEQARAVHASPEAGTHAYR
                     QSLGELIGHFERLMRQTHRMIRHSDRAEREMQALNHQLQALAQQLEYRATHDALTGVF
                     NRSAVIELSNQNLARDSAVMIVLDVDHFKRINDDYGHPTGDAVLAGIVACLRRIVGEA
                     GILGRVGGEEFTVLLPGYKLDEAVRMTETMREAIAGHRFDPPVDRQVTASFGLSDNPR
                     GSGFDIAYNLADAALYAAKRGGRNRVEVSRPERDAAT"
     gene            complement(6642..>6772)
                     /locus_tag="VGC69_19205"
     CDS             complement(6642..>6772)
                     /locus_tag="VGC69_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HEY0297462.1"
                     /translation="SGNRVVLNWHYDAEDETILEFGEELRDDFQAIEFTNRPTSEQ"
BASE COUNT         1058 a         2297 c         2257 g         1160 t
ORIGIN      
        1 ccctgcccga aaccatgatc gagcgcgacg gcgccttcta tgtgcgctcg gtgatgggcg
       61 ggcggcccgg cggcctggcg catttcgcgc ccgaggcgct ggccgagtac gagcgctgcg
      121 cgggcctgcc cggctgggcc acgggggtgt gcgaggacta ccgcgcctcg gccacgctgg
      181 acctggagca cgaccgcgcc ggccgcgccg ccggcgagcg cctgcgccag cccctgcgcg
      241 tgttgtgggg cgcgcgcggc gcggtcggcg ccaatttcga tgtgccgggc ctgtggtcgg
      301 ccgtggcgga tacggtgtcc gggcaggccc tgccggcggc gcattacctg cccgaggagg
      361 tgccggacct ggtgctgcgg gaggcggagc gcttcttcgg cgcggcggac tgaacggcgc
      421 gggcggctca gccccgtatc ccgcgcgggc acgcggggct tgtcaaaagg gacgcgggcc
      481 gggtgctaag cttgcggcat gtccgatgat tcttctcccg ccgacgctgc cccgggcggc
      541 gtcttcacca tgagcgactc gccctgcgtg gccatctgct ccaccctgtt cgacgacgtg
      601 tgccggggtt gcgggcgcac ggccgaggaa gtgtccaact gggtgttcct cagcgaggaa
      661 gaaaagcgcg ccgtgtgggt gcgcatccgg gcccagggat atccgcgccg gccgtagtgt
      721 tgcaagcctg ctgcatgtct tgggcatgtc atatcgggca ggcctaatcc cggcattgct
      781 tacgctgccg gagcccgcat gctcatcgca ctcgtcacgg atgcatggcg tccgcaagtc
      841 aatggcgtgg tccgcacctg gaccaccatg cagcagatcc tgaccagctg gggccaccag
      901 ctggtggtgg tcagccccga gggcgggcgt accgtccccg cgccgtccga gcccgacctg
      961 cgcctgtgcc tgcagccggg caagcagctg cgccgcctcc tgggcgatac cgtgcccgat
     1021 gccctgcaca tcgccaccga gggcccgctg ggcctggcgg cgcgccgcat ggcgctgcgc
     1081 cgcggctggc gcttcaccac ttcgttccac accatgttcc cggagtacct ggaggcccgc
     1141 atgggcatcc cggcgtccct gccctggcgc tacatgcgct ggttccaccg gccttcgcag
     1201 cgcgtgctgg tgcccacgcc cacggtgcgc ggcatcgtgg cctcgcgggg catcgagcgg
     1261 tcgatcgtgt gggcccgagg ggtggatacg cagaagttcc agcccgaggc gcgcgacggc
     1321 ctggccgacc tgccgcgccc catctacctg agcgtgggcc gcgtggcgcg cgagaagaac
     1381 ctggacgcct ttctcgggct ggacctgccc ggcagcaagg tggtggtggg cggcggcccc
     1441 gacgaggcgc gcttgaagcg caagttcccc ggggtgcact tcatgggcgc gcgccccgat
     1501 accgaactgc ccaagttcta ctcggccgcc gacgtgttcg tgtttcccag cctgaccgat
     1561 accttcggcc tggtgatgtt cgaggccatg gcctgcggca cgccggtggc ggcgtttcgc
     1621 aacgaggcgc cgatggccgt ggtcaagccg gggctcaccg gctatctgga cgacgacctg
     1681 gcccgcgcct gccgcgacgc cctcggcctg gaccgcggcg cggtgcgcga gtatgcgctg
     1741 acgcgcacct gggatgccat cgcgcatgat ctgctggccg cgctggtgcc gctggtcgaa
     1801 ggcaccgagt tcagcgcttc ggacctggcg gcggcgcgtt cctgacgccg ttttgccgga
     1861 gcgcatcgtc cgggtacaat ccgcctcagt cctgaggagc gctgcgacgc ccccggatga
     1921 cggcgcaagc ctgtccccgg ggagccgcca ggctcaggaa ccgtcttctg gcgcggtgcc
     1981 cgcaccgcgc tgccgcaacg gcgctcacct ttactgcatc agccggtaaa agtgagcgtt
     2041 gttcccaatg tctcgccttg cccggctggt gtgcgctgat tgcgtactga ttaccgcctt
     2101 taatggagca cgagaccatg accaccaaag ctaccgacta tatcgtcgcc gacatgtcgc
     2161 tggccggctg gggccgcaaa gagctgtcca tcgccgaaac cgaaatgccc ggcctgatgg
     2221 ccatccgcga agaattcggc gccaggcagc cgctgaaggg cgcgcgcatc gccggcagcc
     2281 tgcacatgac catccagacc ggcgtgctga tcgagacgct ggtggccctg ggcgccgacg
     2341 tgcgctgggc ctcgtgcaac atcttctcca cgcaggacca cgccgccgcg gccatcgccg
     2401 ccaccggcac gccggtgttc gccgtcaagg gcgaaacgct cgagcagtac tgggaataca
     2461 cccacaagat cttcgaatgg cccggcgagc aggccaacat gatcctggac gacggcggcg
     2521 acgccacgct gctgctgcac ctgggcacca aggccgagac cgacatctcg gtgctggcca
     2581 agcccacgag cgaagaagag cgcgtgctgt tcgcggccat caagaagacg ctggcgcgcg
     2641 atcccaagtg gtactcgacc cgcctgggcc agatcaaggg cgtgaccgag gaaaccacca
     2701 ccggcgtgca ccgcctctac cagatgtcgc agaagggcga gctggccttc gccggcatca
     2761 acgtcaacga ctcggtcacc aagtccaagt tcgacaacct gtacggctgc cgcgaatcgc
     2821 tggtcgacgg catcaagcgc gccaccgacg tgatggtggc cggcaaagtc gccgtggtgg
     2881 cgggcttcgg cgacgtgggc aagggctgcg cccaggccct ggcggccctg cgcgcgcaag
     2941 tgtgggtgac tgaaatcgat ccgatctgcg ccctgcaggc ctcgatggaa ggctacaagg
     3001 tcgtcaccat ggacgaagcc gccgcgcacg ccgacatctt cgtcacggcc accggcaact
     3061 accacgtcat cacccgcaag cacatggacg ccatgaaaga ccaggccatc gtgtgcaata
     3121 tcggccactt cgacaatgaa atcgacgtcg ccggcatcga agacctgcaa tgggaagaga
     3181 tcaagccgca ggtcgaccac gtgatcttcc ccgacggcaa gcgcatcatc ctgctggccc
     3241 agggccgcct ggtgaacctg ggctgcgcca ccggccaccc ctcgttcgtg atgtcctcgt
     3301 ccttcgccaa ccagaccatc gcgcagatcg agttgttcac gcgcaacgag tcctacacca
     3361 agggccaggt ctatgtgctg cccaagcacc tggacgaaaa ggtcgccatg ctgcacctga
     3421 agaagctggg cgccaagctg accacgctct ccaaggaaca ggccgactac atcggcgtgc
     3481 cggtgcaagg cccgttcaag gccgaccact accgttattg atatgctgat agcctgttgt
     3541 ttgagttctt gagacgttcc caggttgccg ggcaggcccc ggcgccctcg gcccctgcgg
     3601 ggcttccccg ccacagaggc gccgccaggg cctgcccggc aacctgggaa ctgcgcaaaa
     3661 atcgtgcaac gggtattgtt aactgcttga gcatgggaga gacaaaatga gcctgatact
     3721 cgtctggatt ctgaacgccg tggctttgct ggtcgtggcc tatttgctgc ccggcatcac
     3781 cgtggccagc ttcggctccg cgctgattgc cgcgctcgtg ctgggcctgc tcaacatgct
     3841 ggtcaagccg gtgctggtgc tgcttaccct gcccatcacc atcgtcacgc tcggactctt
     3901 cctgatcgtg ctcaacgccc tgctgttctg gcttgccggc tccatcctgc gcggcttcca
     3961 ggtctccggc ttctggtggg ccgcgggcgg cgcgattctc tacagcatca tctccggact
     4021 gctgtctcgc atcattccct gacggccgcc catgactgca acgacccagg cctacagcct
     4081 cgaattcttt cccccgcgcg acccggccgc ccacgagcgc ctggtcgaga cggccaggca
     4141 actgctggtc gtccagccca aatacgtcag cgtcaccttc ggcgccggcg gctcgaccca
     4201 ggccggcacg tccgaggccg tgcgcacgct gcgcggcatg ggctgcgacg cggcgccgca
     4261 cctgtcgtgc atgggcgcca cgcgcgccag gctgcgcgag atcctgcagg cctaccaggc
     4321 cgagggcgta aagcgcctgg tggcgctgcg cggcgacctg ccctcgggca tgggcggcga
     4381 cagcggcgaa ctgcgccatg cccgcgacct ggtggccttc atccgcgcag aaaccggcta
     4441 ctggttccac atcgaagtgg cggcctatcg cgagatgcac ccgcaggccc acaatgcctc
     4501 gaccgacctc gcgcacttcg cagacaaggt caaggccggc gccagctcgg ccatcacgca
     4561 atacttcttc aacgccgacg cctatttcga cttcgtcgag cgggcgcggt cattgggcgt
     4621 ggacgtgccc atcgtgccgg gcatcatgcc catcaccaac tccagccagc tgctgcgctt
     4681 ctccgacatg tgcggcgccg aagtgccgcg ctggatccgc ctgcgcctgg ccgagttcgg
     4741 cgacgacaag gcctccatcc gcgctttcgg ccacgaggtg gtgaccggct tgtgcgaacg
     4801 cctgttgcag ggcggcgccc cgggcctgca tttctatacg ctgaaccagg cggaatcgac
     4861 gctgggcgtc tggcgggcat tgacgggcag ataaagcccc cacgccgcgc cttcggcttg
     4921 ctgcccggcg gcaaaaatgg cccatctacc tattcacgac gcctgcttgt ccccgttgct
     4981 ttgcgccagc acctgcagcg gcaggtactg ctcggccccg atggcggccg attccacccg
     5041 caccggctgc tgcgagcggc gcatcggctg cagggcgtcg accgcggcgg cggccgtcca
     5101 ggccggatcg ggaatctcgc cgaacacctg ccgcaggcga ttgctccagg tggtgcggat
     5161 catgcggaaa tactcgttgt cttcgtcgat gcaggcgaag tgcgtcacgc gcagcgcgcc
     5221 ggtctcgtag accagcaggt cgagcggcag gcccaccgaa atattcgagc gcagcgtcga
     5281 gtccatggaa atcagcatgc acttggccgc ttcgtccagc gtcgtgccgg gggcgagcac
     5341 gcggtccagg atgggcttgc cgtacttggc ctcgccgatc tggatatagg ggcattcgtt
     5401 gctggcttcg atgaaattgc ccgcggcgta cacctggaac aagcggcagg gttcgttgcc
     5461 gatctggccg ccgaaaatga tgttgacgtt gaattccacg ccttgttcgg cgagcgaagc
     5521 ggcgtcgcgg cggtggactt cgcgcacggc ctcgccgatc cggcgcgcgg cctcggccat
     5581 attgggcgcc tgccagagcg aatcggggtg gctggcatcg tgctgcctga gcaggttgac
     5641 cacggcctgg ctcagggcca gattgccggc cgtcatcagc accatcatgc gttcgccggg
     5701 ctgctcgaac actttgagct tgcggaatac gctgatctgg tcgacccccg cgttggtgcg
     5761 cgaatcggaa agaaagacca ggccggagtt caggcggacg gcgacgcaat atgtcatgac
     5821 ggttggcttt gacggattgc gcacattgta agcaaagccg cgcttcaggt cgcggcgtcc
     5881 cgttcggggc gggatacctc gacccggttg cgtcctcccc gcttggcggc gtacagggcg
     5941 gcatcggcca ggttgtaggc gatgtcgaag ccgctgccac gggggttgtc gctgaggccg
     6001 aaactggcgg tgacctgccg gtcgaccggc gggtcgaagc ggtggccagc gatggcctcg
     6061 cgcatggttt cggtcatacg cacggcctcg tcgagcttat agcccggcag cagcacggtg
     6121 aattcctccc cgcccacccg gccgaggatg cccgcctcgc cgacgatgcg gcgcaggcag
     6181 gcgacgatgc cggcgagcac cgcgtcgccc gtgggatggc cgtagtcgtc gttgatgcgc
     6241 ttgaagtggt cgacatcgag cacgatcatg acggcgctgt ccctggccag gttctggttc
     6301 gacagttcga tgacggcact gcggttgaac acccccgtca gcgcgtcgtg ggtggcgcgg
     6361 tattcgagtt gctgggcgag cgcctgcaat tgatggttga gcgcctgcat ctcgcgttcg
     6421 gcgcggtcgc tatggcggat catgcggtgg gtctggcgca tcaggcgctc gaaatgcccg
     6481 atcagctccc ccagggattg ccggtacgcg tgcgtgccgg cctcgggcga ggcatgcacc
     6541 gccctggctt gctcgagcgc ggcgttctcg ctctcgaaga gatccggcgc gctgtccgcg
     6601 atcgcatccg gagaggggcc tgatctggac atcggcgctg tttattgctc ggaggtgggg
     6661 cggttggtga actcgatcgc ctggaagtca tcccgcagct cttcgccgaa ttcaaggatg
     6721 gtttcgtcct cggcgtcgta atgccagttc aggacaacgc ggttgcccga tt
//
LOCUS       DASWPO010000359        29017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_800717, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000359 DASWPO010000000
VERSION     DASWPO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 29017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29017
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800717"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1162
                     /locus_tag="VGC69_19210"
     CDS             47..1162
                     /locus_tag="VGC69_19210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0297463.1"
                     /translation="MSDSRSAAVIVIGGGLHGSSAALHLARAGVQALVIEKNYVGRHA
                     SGVNAGGVRTLARHVAEIPLALASRELWFRIEELVDDDCGFEQHGQVRVAENEADVAV
                     LGERLRLMQSLGHTHETWIDADELRAMIPKIAPSVRGGVIAREDAAADPYRTTAAFRR
                     KAASLGARFLEGVRVHEVRRDGAQWAVATSAGGYTAPIVINCAGAWADRIAAQWGEPV
                     PLQPISPMMLVTLRMDHFLDPVVIGTGRPLSFKQRTNGTVLIGGGRRAWLDRDAEWTE
                     LDFRELGAGARTVCDLFPHMKDAIVNRGWAGIEAAMPDEIPVIGPSSTREGAFHAFGF
                     SAHGFELGPIVGRIMAELVTRGETSLPIGPFSIRRFA"
     gene            1218..2165
                     /locus_tag="VGC69_19215"
     CDS             1218..2165
                     /locus_tag="VGC69_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0297464.1"
                     /translation="MQDLQILLQSYGSWLVFLNVLVEQAGLPIPSYPILIASGAMSDG
                     PVGWISAWLLATLACWAADSVWYWAGGRYGGGLLSMICKVSLSQDSCIRQTQKLFLRI
                     GVRALLVSKFLPGAGALTNVMNGFAGTPYRRFLAFELPGALIWSGSGLLLGAVFRGVV
                     NQVIGLIGQYGKWGLLLLVFVLAAYLVVRAFRRWLLVRSLRVVPRLPVDELLQWQAAG
                     RTPVVIDVRPGLDSLLRIPGAIVADLRSPIPDLALEEDTPIVVYCACPNEISAARLAA
                     RLQAAGYRNTWALLGGYEAWERKARAVSGTPAVQEAGGA"
     gene            2232..3167
                     /gene="panE"
                     /locus_tag="VGC69_19220"
     CDS             2232..3167
                     /gene="panE"
                     /locus_tag="VGC69_19220"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0297465.1"
                     /translation="MRILVLGAGGTGGFFGGRAAQAGVDVTFLVRPARAALLREKGLR
                     IKTKDGTDVIQPKLVTADALGGPYDVVILSCKAYDLDGAIESLKPIMQQNPKAAILPI
                     LNGMRHYDALDAAFGKERVLGGLCQVTATIGPDGEIVIMGPFGAIIFGEREGGLSERG
                     EAIRQALEPASFTTKLSADIYQDVWEKYTFLCTLAAATCLMRGSVGEIVRAAGGEHFM
                     RSLLIEINGVAQGHGHGMRPNAEAFTVGALTDPASGVTASMFRDLRQGGQIEGEHLIG
                     DMVQRAEKLGLKAPCLRTAYTHIQVYLAQRAAQQA"
     gene            3164..3418
                     /locus_tag="VGC69_19225"
     CDS             3164..3418
                     /locus_tag="VGC69_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297466.1"
                     /translation="MSAVPGFEPVELARSYLLLNHGPTVLVGSAHAGRRNVMAAAWAD
                     PQAFSGNRWHFASDARRSLHYVAGGTFFATGEPFSVPVET"
     gene            3422..3922
                     /locus_tag="VGC69_19230"
     CDS             3422..3922
                     /locus_tag="VGC69_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEY0297467.1"
                     /translation="MRRIYLAGFDVFRADAVAQGERLKALCLAHGFEGLYPLDNALPA
                     GLAGRAAAQWIYEQNIGLIRRADLVMANLNAFRGLEPDSGTAFEVGYGAALGKPVWAY
                     TGDARPLVAQAGAGRDPRDASRVVDAQGYSVEDFGLNVNLMLACAARVVAGDAQACLR
                     AMAAAG"
     gene            complement(3975..4682)
                     /gene="ribB"
                     /locus_tag="VGC69_19235"
     CDS             complement(3975..4682)
                     /gene="ribB"
                     /locus_tag="VGC69_19235"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0297468.1"
                     /translation="MSHLTASNTSLFNQPFAARLAQALQQLRLGRPIILMDDADRENE
                     ADLIVAADTLTVPVMAQLIRDGSGIVCLCLPGETLDRLGLAPMVAHNGSRHGTAFTVS
                     IEARQGVTTGVSAADRVTTIQAAIAPAAAGTDLVSPGHVFPLRAQPGGVLARRGHTEG
                     SVDLAALAGLSPAGVLCELMNPDGTMMRGAELVRYAAAHGLVALHIAELADHLRDLRQ
                     AKAKGADAEREAETHAA"
     regulatory      complement(4785..4930)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4989..5480)
                     /locus_tag="VGC69_19240"
     CDS             complement(4989..5480)
                     /locus_tag="VGC69_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY0297469.1"
                     /translation="MTTDTAKPEKPWHVVRRSKLHGNGIFAARKIPEGTRIIEYLGAR
                     ISDKEADRRHPTNPDDPFHTFFFSLSSGKIIDGGDHGNDARWINHSCEPNCESQEGTG
                     GKRVYIVALRDIKRGEELFYDYGLVLDDRITKKLKENYRCLCGAPSCRGTMLALPKKK
                     KKK"
     gene            complement(5500..5976)
                     /locus_tag="VGC69_19245"
     CDS             complement(5500..5976)
                     /locus_tag="VGC69_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0297470.1"
                     /translation="MHLDKFDLAILKVLQDDARASLNDIGAAVGLSATPCWNRIKRME
                     REGVIRGYTVDIAPETLGFMDTVIVHVTLESHSEETLYEFGRALAEIPEVLEAFLVSG
                     DYDYYIRIAVRDTRDYERLLRERLYRIPGIRHSKSGFVLRSLKESRLPLSVVTPAA"
     gene            6071..7939
                     /gene="ilvB"
                     /locus_tag="VGC69_19250"
     CDS             6071..7939
                     /gene="ilvB"
                     /locus_tag="VGC69_19250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY0297471.1"
                     /translation="MVGMNDRLSTPLLADLDATDRDDFPAGAPPANGARRLLDALIAC
                     GVDTVFGYPGGAVLPLYDALHAEPRIRHVLVRHEQAAVHAAEGYARTTGKPGVVFVTS
                     GPGMANTTSGLLDALCDSIPVLCISGQVATGAIGTDAFQECDALGISRSVTKWNVQIR
                     AVEEVADIVARAHALTRQGRPGPVLVDFPKDVQLAAEPAAREAGDPAARVRCLAALRA
                     QRQAGKRVQRIPQGAVQRAAALIAQARRPVFYGGGGLINAGPEACAAFTALVRAVGAP
                     CTLTLMGLGAYPASDAQFLGMLGMHGTLEANLAMHQADLVVCVGARFDDRITGKLADF
                     CPHARKIHLDIDPASINKVVRADEALVGDAGKLAQALRDALARHDLPAARLAGWWTRI
                     EGWRARRCLDYADTDDANLPQRLMRRLDAALAGRDAIVSTDVGQHQMWAAQYLRFDRP
                     NRWLTSGGAGTMGYGLPAAIGAQVAHPDAAVVCVSGDASVLMNIQELATAVQHRAPVK
                     VVLCNNGYMGMVRQWQELIHGGRYSHSYNEALPDFVALARAFGWQAERVGDPAQLDAA
                     LARCLDHAGPYFLDVVVASQENCFPMIPAGCGHDRIMLAEGVWHEEPASAGSSSFD"
     gene            complement(7973..8584)
                     /locus_tag="VGC69_19255"
     CDS             complement(7973..8584)
                     /locus_tag="VGC69_19255"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0297472.1"
                     /translation="MSSYFYDPAQGHGLPHDPLKAIVAPRVIGWISTRSAQGVLNLAP
                     YSFFGIFSTSPPIIGFSSEGTKDSIRNIEETGEFVWNFTSRALAEQMNRSSAPVAPEV
                     DEFALAGLTPAAGRQVSVPHVGQAPAALECKLLQVIRLHTLDGRPMENRLALGQVVGV
                     HIQEAFLKDGLFDTWAAQPVMRTGYQADYAAIGEQFRMIRPKA"
     gene            8702..9745
                     /locus_tag="VGC69_19260"
     CDS             8702..9745
                     /locus_tag="VGC69_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0297473.1"
                     /translation="MTRKAASRSSIVEVARKAGVSPATVSRAFNQPDLLRPATLARIE
                     AVARSDGFRPNRVGRSLRAGSTRTIGLLLPTLGNPVFAECFEGAEQHARQAGYSVMMA
                     TTGYAPEVEVTAVQTFIDHQVDGLILTVSAPARNTALAMLQAAELPHVLAYNESGTHP
                     FVSVDNHAAARDMMQALAALGHRRVAMVTGPLSASDRARRRLDGARVQAGALGLDEVA
                     HLVMPAHTAASLELLQAALRGPAAPTALFCSNDLLAASVITHLVAMGLSVPRDLSVCG
                     FDGMAIGALMVPPLTSVEQPSRQIGATACAHLLTRLLGGQTSSVRLPHRILSGGTVAA
                     PPLSTRTARRKTA"
     gene            9742..10755
                     /locus_tag="VGC69_19265"
     CDS             9742..10755
                     /locus_tag="VGC69_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297474.1"
                     /translation="MKRMINTALRCALAALALGGGAAQAAETAICYNCPPEWGDWAGQ
                     IKAIKDATGIVVPPDNKNSGQALAAIAAEKGNPVADVTYLGVTFGYQAAKDGLVQSYK
                     PAHWDDIPAGLKDPDGKWFAIHSGTMGFMVNVDALHGKPVPKSWADLLKPEYKGLVGY
                     LDPASAFVGYVGAVAVNRALGGALDNFGPGIEWFKKLQANQPIVPKQTAYARVISGEI
                     PILLDYDFDAYRAKYTDGANIDFVIPSEGTITVPYVMSMVANAPHAANAHKVLDFVLS
                     DKGQALWANAYLRPVRASAMSPEAQKKFLPDSEYARAGTVDYLQMANAQRAFSDLYTK
                     EVH"
     gene            10757..11575
                     /locus_tag="VGC69_19270"
     CDS             10757..11575
                     /locus_tag="VGC69_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0297475.1"
                     /translation="MVHRRGWLVFAAPAAALFLAFWLFPMAGLALRGASPVDGRSAYL
                     IVVTEPRYWRSLFNTAALSLAATLGTLLVSLPVGRFLAWHRDFPGRRILVGLLMFPLA
                     FPGVVVGFLIILLAGRQGVVGQFTQWLTGEPLVFAYNMGGLFLGYLYFSLPRTIGVIE
                     AAASQMDASLLEAARTLGASAWRRFVDIELPALRPALIGAGAMGFATNMGAFGTVFTL
                     GTRIDVLPMTIYNEFTNYANIPVAAALSILLGLVTWVALYIAHSVAGSSAGAGA"
     gene            11628..12443
                     /locus_tag="VGC69_19275"
     CDS             11628..12443
                     /locus_tag="VGC69_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0297476.1"
                     /translation="MSTLHTKADARLGLAVTLAVAAFLIGPVVLSILAGLTKNYFVGI
                     ASGLTLRWVAQVWDLYADTIWRSLFVALLTLAICLLAGVPAAWVLTLYRGRLARALEE
                     LLTLPVAVPGLASALALIVSWGTVTGLRTSSLFIVIGHVLFTLPFMVRSTRASMAGAG
                     LAVLDEAAATLGASRLTRFLTVVVPNARAGILTGGLTVLTLSIGEFNLTWLLHTPLTQ
                     TLPVGLADSYASMRLEVASAYTLVFLLMLMPLLVGMQWLAGQGTRRAAGGKGA"
     gene            12498..13478
                     /locus_tag="VGC69_19280"
     CDS             12498..13478
                     /locus_tag="VGC69_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297477.1"
                     /translation="MNKTSITLRECAKTWPDGTRALQPLDLRVEGGEILALLGPSGCG
                     KTTLLRLICGLETSDAGGKIYYGDDDITHLSPERRGAGVVFQSYALFPNMSVADNVAY
                     GLRVRGQDAARRKQRALDMLALVRMQDYADRNVTQLSGGQRQRVALARALAIEPRVLL
                     LDEPLTALDAKLREHLRVELAQMLRELGVTTVIVTHDQDEAMMLGDRIAVMSAGRIEQ
                     IGTAETLYRQPATDFVGEFLGTLCHVRAATADGLLVPGASELAFRPHHAALLAPQPES
                     LQARVAARFFLGGTVRYELALPDGQRFSAMAPADSAHQAGDAVSIKLVRS"
     gene            13511..14332
                     /locus_tag="VGC69_19285"
     CDS             13511..14332
                     /locus_tag="VGC69_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEY0297478.1"
                     /translation="MLIAQISDLHIRMPGQKAYRVVETDRYLPPAVEALNALDPLPDL
                     AILSGDLTDFGRHVEYAHLRRMLDGLRMPYYLLAGNHDERLQLAQSFDHLPYLQGTGE
                     FLQYTVEDHPVRFIMLDTVVPKASHGELCGRRLAWLADRLAEQPGRPTIVAMHHPPFA
                     TGIAHMDEIGLLRGAPAFKALVARYPNIERILCGHLHRTIFRRFAGTVVSTCPSPAHQ
                     VVLDLRPQGPSAFIMEPPGFQLHHWCDGALNTHHAYIGDYPGPYPFHEDGMLIDE"
     gene            14436..15476
                     /locus_tag="VGC69_19290"
     CDS             14436..15476
                     /locus_tag="VGC69_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297479.1"
                     /translation="MLVLENLGKQYAGNVAVDGVNLHAPAGSFTALLGPSGCGKSTTL
                     RMIAGLDAPTAGRILIGGRDVTQLAPAQRRIAMVFQSYALFPHLSVRENILFGLRVRK
                     EPERDFDRRLSRVAGLLGLSALLDRRPAQLSGGQQQRVALGRAVISEAPVCLMDEPLS
                     NLDAQLRQDMRREIRGLQQALGITMVYVTHDQTEAMSMADQVVLLNAGRIEQQGTPQA
                     LYARPATPFAARFIGTPPMNLIAIEPLFGQSVIRGTHGPAIAGAPGHARLLGLRPEHL
                     RLDASGVPAEIESVEYFGADSIALCRVGDGRIAVRVGGHLRAERGTRTGLAWNPGHQH
                     FFSADPADTAAA"
     gene            15514..16800
                     /locus_tag="VGC69_19295"
     CDS             15514..16800
                     /locus_tag="VGC69_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297480.1"
                     /translation="MQRIVLKTLAASLAGAFFALPAYAATQVEFYYPVAVGGPITKIV
                     DDLAAQFNKENPDIVIKPVYSGTYQDTIAKALTALKGGTPPQLAVLLSTDMFTLIDED
                     AIAPIDSLAQSDDDKKWLGGFYDAFMANTRANGHTWGVPFQRSTIVAYYNKDLFKEAG
                     LDPEQGPATWAELVDDAKKLTKKDASGNVTQWGVEIPSSGGYGYWMLQALATPNDALL
                     MNQAGTETYLNKPAVVEAAQYWYDLAYKQGVMPKGTTEWATIPKDFLEKKAAIVWTTT
                     GNLTNIATNASFPFGVAMMPKNKRGGSPTGGGNFYVFKQGTPAQQQAALKFAKWATSP
                     EHAADWGIATGYVAVTPAAWETPKMQAYVQKLPAAAVARDQLAVSVAEFSTHDNQRVT
                     KVVNDAMQAIMTDAKKPRQALDDAQKEAGRLLKSYQ"
     gene            16826..17704
                     /locus_tag="VGC69_19300"
     CDS             16826..17704
                     /locus_tag="VGC69_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0297481.1"
                     /translation="MNHRRLDAVYGWLMLLPAVVLLAAFTHYPAVATVWDSLFSTPKG
                     VRPAHFVGLDNYLGMFDDDVFWQAWWNNLAYAAVTVPVSIAVALIMALWVNGRLRGRG
                     FLRMAYFTPTVLPMVAVANIWLFFYTPQYGLIAQVMHGFGLPGYNWLGSRGTALPALM
                     LVAVWKESGFFMIFYLAALQTLSPSLREAALLEGASRWQYFRRVLWPMLMPTTLFVLV
                     NAVINAFRLIDHVVVMTHGGPDNASMLLLYYIYQVGFSFWDTAYAATLTVVLLTVLAV
                     MALVKFRWFDRKTHYQ"
     gene            17707..18507
                     /locus_tag="VGC69_19305"
     CDS             17707..18507
                     /locus_tag="VGC69_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY0297482.1"
                     /translation="MTRKLDTIGAWLLGLLWILPLAYAAWAAFHPPAYATRFELLAPL
                     TLENFKRAWAAAPFARYFLNTFMLVTMVLAAQFVLSTLAGYAFVRFNFRGRDFLFLMV
                     LLQLMVMPDILLVENYRTMSWLGVRDTVFAIGLPYFASGFGIFLLRQTFKTVPRELEE
                     AARVEGANPLQVLMKVYVPLAKPTYVAYGLVSVSTHWNNFLWPLIITNSVGARPLTVG
                     LQVFSGTDQGVDWSVITAATLLSAGPLLVAFLLFQRQFVQSFMRAGIR"
     gene            complement(18518..19453)
                     /locus_tag="VGC69_19310"
     CDS             complement(18518..19453)
                     /locus_tag="VGC69_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY0297483.1"
                     /translation="MPHPPRKVIITCAVTGAIHTPTMSPHLPITAQEIADAAVEAAEA
                     GAAIVHLHARDPETGKPDQSPEAFAPFLKDIKQRSNCVINITTGGAPTMLVQERVRPA
                     AHFKPEVASLNMGSMNFGLYPMLARHNEFKYDWEKPYLEGSKDRIFRNTFTDIEYILT
                     TCGENGTRFEVECYDIGHLYTLAHFVERGIIKPPLFVQSVFGLLGGIGTHPEDVAHMK
                     RTADRLFGDDYRWSVLGAGRSQLPIAAMSAAMGSNVRVGLEDSLWIGRGRLAESNAQQ
                     VRAARQIIEGLGLEIASPDEARKMLALKGRDAVGF"
     gene            complement(19501..20046)
                     /locus_tag="VGC69_19315"
     CDS             complement(19501..20046)
                     /locus_tag="VGC69_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297484.1"
                     /translation="MTSRHDVPNPAEIGANPLDKPDAYGNDAPTSKAVSRNRNGFVIA
                     RKFGNLKAEDDAPSVNSKPEYVRLACHANPRPAAEGTIRYIYLLGTPSWNPKTHPYRP
                     PSGDSATDERLVLGTACGKRHSRHLPRTGRRLRPPMRRSAATLRCLPAGDALAEFDTA
                     FLPDTLANARTILALLASLGI"
     gene            20258..21091
                     /locus_tag="VGC69_19320"
     CDS             20258..21091
                     /locus_tag="VGC69_19320"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0297485.1"
                     /translation="MKEDDISAPADAEDEGGHRAAILHDDIYSTLRHDLVTGGLVPGQ
                     RVSIRTLAERFETSFLPVRDALKRLVAEHALVMLPNRTVLVPMMTRERFQELLQVRLM
                     LETMLSRQAAARIVHDEVVKLDAINEAMQQAVRDGDVRRYLAANHAFHFGLYEAGGSE
                     VALPIVDKLWVQVGPFLNAVFNQQGTRSAGGNHAEVLKALRRHDPIGVAEAIHSDLAN
                     AADLILANVEFPEWAYEPKPRRMKHPNGGAHEQEALSQDGPKAAVSPDRRASGRKANQ
                     E"
     gene            21128..22339
                     /locus_tag="VGC69_19325"
     CDS             21128..22339
                     /locus_tag="VGC69_19325"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0297486.1"
                     /translation="MTKKPIMPAVLAALTAAASLAWPGTGRAEPGVTDTEITIGMFAP
                     LSGPLVAFGIDPLAAAKMLYEEVNKAGGINGRKIKTLVEDDKCNPNDLVAVVKKFVTV
                     DKVFLLHGGSCTAAISAASDYINREKVPFAMLNAAGDNSVFPPTRYVFGSFQGTQRTY
                     SAVFARFAGEYLKAKRAAIIVHDDEYGKANASMAKATFDKIGVETVDIERIAPDISDV
                     TAPMLKIRAAKPDVILSGVYPAPAVLVAQKYGEFGMTGIPLLQAAQGIPTPSVFAKNV
                     ANPAALQNFYYTWAFNDIGDAAVREKYINMFKAYNNGREPGPFMTSGLPSAMAVIEAL
                     KAAGRDLTREGFITAMEKLDYQSDIMPGPLQFGPNRRDAARTMRIIKYDGVTQQVMPG
                     SFSWDGKQPTE"
     gene            22398..23282
                     /locus_tag="VGC69_19330"
     CDS             22398..23282
                     /locus_tag="VGC69_19330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0297487.1"
                     /translation="MQAILSGIVIGCVYALIALSLVIIYKSTDVVNFAAGEFVMVAAY
                     AGLLLLDHYKINYAVMTVLAVGLAFVLGGLFERVALSRIIGFKYATEAALVPMVVATI
                     GLSFMLKGAVRVIPSTQEVLRLPPIFSGPPVFFGDLVLQRQDIGIVGVAVLVMILLSL
                     GFNLTATGKALRATSQNSRAAALIGIPVKGMRTMAWAVAAGLAGISGILLAPKLLMTP
                     DMGSVFMMGIAASIVGGFTSLVGCVIGGILLGVLQNLVGIFVSPQAITVTPFFIIMLV
                     LVVRPNGLFGEAMRLKKV"
     gene            23284..24297
                     /locus_tag="VGC69_19335"
     CDS             23284..24297
                     /locus_tag="VGC69_19335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0297488.1"
                     /translation="MYGSPRYPELAGLLVIVAVGFVLPWLLPEYFLYTCNMLMTYAIL
                     AIGLDLLLGWSGQFAFAHIAFFGIGIYGTALLESRLGVPYVLSVAASAGLAGLLGIVI
                     GLPATRLRMVYLALATYAFAECAQWVFRSWDQVTGGSDGLRFPPPDIFGYTTGTDSRA
                     FPVVALMLALVLGAALYLTRSSLGRQMCAIRDSEHVAAASGIDVRRIKVIAFAISAVF
                     AGIAGGTYTLFQSFVNADVLGASQLVTVLTMIVIGGSGSIVGVLLGVAVIGLLPELLR
                     IMPQSMLVWQEFIYGAILVLSVVGMPKGLWGLIKPRFGKKSKETKTAPVPPTHSTFTE
                     IAE"
     gene            24294..25061
                     /locus_tag="VGC69_19340"
     CDS             24294..25061
                     /locus_tag="VGC69_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297489.1"
                     /translation="MSAFLSLDDISVRFGGLKAVDRFSMEVQEGSIHALIGPNGAGKS
                     TVFNCISRFCTPSEGGIHFDGRDLLSLPAHRVVSSGIGRTFQNLELLKRMTVLENVLI
                     GQTAHVPRYVSFGPGAKRKRTEAQAVREAEAVLEETNLLPYRDTLAGELDFGHQKLLD
                     LARALAGKPRLLLLDEPAAGLRNREISFVDELLVKLAHSRRLTIILVEHVMPLVMSIA
                     DRITVLNFGQKIAEGAPAEIRCHPKVIEAYLGSDAHA"
     gene            25054..25758
                     /locus_tag="VGC69_19345"
     CDS             25054..25758
                     /locus_tag="VGC69_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297490.1"
                     /translation="MLRLSNIEAFYGSVQALDNVSLEVGDSQIVCLLGANGAGKTTML
                     NCVSGLVAAPRGKITFDGEDITNIGAEGVVARGIVQVPEGREIFPAMNVEDNLVLGAW
                     QKKQSRRHTREQLERIYGLFPRLRERARQHAGTLSGGEQQMLMIGRALLAEPKLIMFD
                     EPSLGLSPLLVQQVFGIIRKIHEQGMPILLVEQNARLALKVSSYCYVIENGEIRFHGP
                     SQALMENEEISKAYLG"
     gene            25779..26552
                     /locus_tag="VGC69_19350"
     CDS             25779..26552
                     /locus_tag="VGC69_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0297491.1"
                     /translation="MTKLSEYKDKYECIKFERRDDILQMTVHTNGDSLRWGLVPHREL
                     PMAFFDVANDYENKVVILTGTGAEFSGPQVIPSDGHPIFPTRPKMELVDALLSEGKQG
                     LLNFLQIDVPVITAFNGPAWRHGEIPLMADIVIAADTIAFQDSAHFQGGLVPGDGVHV
                     VYPLLLGVNRARYFLLMGQILRAKEALEYGLVSEVLPPDRVLPRAWEIARNMASKPKS
                     LLRASRAVLTEKLKRDAQDMISFGLYAELLALQDKPDAI"
     gene            26568..26879
                     /locus_tag="VGC69_19355"
     CDS             26568..26879
                     /locus_tag="VGC69_19355"
                     /inference="COORDINATES: protein motif:HMM:NF014479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HEY0297492.1"
                     /translation="MLFALKIEVSVPADVPQETKADLRKRENARAIELIKQGRLKRIF
                     RVVGEVSNISLWEAETLEQLHADVGSLPLYPYMKVHVTPLIEHPVTAAWKETNGALPP
                     F"
     gene            26929..28164
                     /locus_tag="VGC69_19360"
     CDS             26929..28164
                     /locus_tag="VGC69_19360"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0297493.1"
                     /translation="MSTNSITVFKGDTSRLKRMITVPDRMSPEGYRLELEKIFSRAWL
                     PTLSVSDVPENGSYTVVDVPPKKTSVIVVRGNDGVVRAFQNVCRHRGDMLVHKGNGKT
                     KQFVCGFHGWRYSTEGALAGVTDETQFPDIERDKLGLVPVNCEVWNDLVFLNFEKTPH
                     VTLKEWLGGIWDEYSGYAEGREKVADHRVVLKANWNVAVNAFCEGYHNMYIHKYTVPD
                     YQGGKGNPLRHRAYIEIGKRFGRYSAHANMDHKRTPAEAVLYGRSKGLFPSFPKTDPN
                     TLTPGINPSRFGEWAFDIVHLFPHFVFNPTANSHQYMWFWPVDAEHVEIRVMSYMFKA
                     RNAADRLAQAYSKVRLREVLREDLATMEAASRMISTGALPCMVFSEQEMLIQNHYTIA
                     DEMTGRNEPSVDRYAKAAA"
     gene            28180..28539
                     /locus_tag="VGC69_19365"
     CDS             28180..28539
                     /locus_tag="VGC69_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297494.1"
                     /translation="MTNQKLPDGFADLEPFVAEWAIDNEKDRYLKLHASQIEHLRDFY
                     NMVFERLDQIIERLNQTPLSQFSEQERTLMNLAMTFAETAHPIDLKWQGVDFNDAYTW
                     EKFDFRGVSLAPMRETA"
     gene            28539..>29017
                     /locus_tag="VGC69_19370"
     CDS             28539..>29017
                     /locus_tag="VGC69_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEY0297495.1"
                     /translation="MEMVIAARRMEAEDIVSLDLVAPAGGSLPPFEAGAHVDVQVTPA
                     ITRQYSLSNDPAETRRYRLAVLAEPDSRGGSAGVHAGLPTGSRVTIGMPRNLFRLDES
                     AGRSILLAGGIGITPLLAMAYRLRTLGRDFELHYCVRGESRIAFRAELAAGPLARHV"
BASE COUNT         4681 a         9653 c         9577 g         5106 t
ORIGIN      
        1 cggatcaaga aaaagggcgg cgcgtagcgc cggcggggcg gcttccatga gcgactcgcg
       61 cagcgcggca gtcatcgtca tcggcggtgg cctgcacggc agctcggccg ccctgcacct
      121 ggcgcgcgcc ggcgtgcagg cgctggtgat cgagaagaac tacgtgggcc gccacgcctc
      181 gggcgtgaat gccgggggcg tgcgcaccct ggcgcgccac gtggccgaga tcccgctggc
      241 gctggcctcg cgcgaactct ggttccgcat cgaggagctg gtcgacgacg actgcggctt
      301 cgagcagcac ggccaggtgc gggtggcgga gaacgaggcc gatgtcgccg tgctcggcga
      361 gcggctgcgc ctgatgcagt cgctgggcca tacgcacgag acctggatag acgccgacga
      421 actgcgggcc atgatcccga agatcgcgcc ttcggtgcga ggcggcgtga tcgcgcgcga
      481 ggacgcggcg gcggacccgt atcgcacgac cgcggccttt cgccgcaagg cggccagcct
      541 gggcgcgcgt ttcctggaag gcgtccgggt ccacgaggtc aggcgcgacg gcgcgcagtg
      601 ggcggtcgcg accagcgcgg gcggctacac cgcgcccatc gtgatcaatt gcgccggcgc
      661 ctgggccgac cgcatcgccg cgcaatgggg cgagcccgtg cccctgcagc ccatcagccc
      721 catgatgctg gtcaccctgc gcatggatca tttcctcgac ccggtggtga tcgggacggg
      781 ccggcccttg tcgttcaagc agcgcaccaa cggcacggtg ctgatcggcg gcgggcggcg
      841 cgcctggctg gaccgcgacg ccgaatggac ggagctggac ttccgcgaac tgggcgcggg
      901 cgcgcgcacg gtgtgcgacc tgtttccgca catgaaggac gccatcgtca accgcggctg
      961 ggccggcatc gaggcggcca tgcccgacga gatcccggtc atcggcccaa gctcgacccg
     1021 ggaaggcgcc ttccatgcct tcggcttctc ggcgcacggc ttcgagctgg gccccatcgt
     1081 cggccgcatc atggccgaac tggtcacgcg gggcgagacc agcctgccta tcgggccttt
     1141 cagcatccgc cggtttgcct gagccataat aggcgcgaac cttccccggc ccccgcgccg
     1201 atctttccac ggctttcatg caagacctgc aaattctgct gcaaagctac ggcagttggc
     1261 tggttttcct caacgtgctg gtcgaacagg ccggcctgcc gattccttcc tatccgatcc
     1321 tgatcgcttc gggtgcgatg tcggacggcc ccgtcggctg gatatcggcc tggctgctcg
     1381 ccaccctggc ctgctgggcg gccgattcgg tctggtattg ggcgggcggg cgttatggcg
     1441 gcggcctcct gagcatgatc tgcaaggtgt cgctgtcgca ggattcctgc atacgccaga
     1501 cccagaagct gttcctgcgg atcggggtgc gcgccttgct ggtttccaag ttcctgcccg
     1561 gcgcgggggc gctcaccaat gtgatgaacg gtttcgccgg cacgccgtat cggcgctttt
     1621 tggcgttcga gctgcccggg gccctgatct ggagcggttc ggggctgttg ctgggcgccg
     1681 tgttccgcgg cgtggtcaac caggtgatcg gtctgatcgg ccagtacggc aagtggggcc
     1741 tgctgctgct ggtgttcgtg ctggcggcct atctggtcgt gcgcgctttc cgccgctggc
     1801 tgctggtgcg cagcctgcgc gtggtgccgc gcctgccggt cgacgaactg ctgcaatggc
     1861 aggccgccgg ccgcacgccg gtggtgatcg atgtgcggcc cggcctggat tccctattgc
     1921 gcatacccgg cgccatcgtg gccgacctgc gatcgcccat tcccgatctg gccctggaag
     1981 aggacacccc catcgtcgtg tactgcgcct gtcccaacga gatctcggcc gccaggctgg
     2041 cggcccggct gcaggctgca ggctaccgca atacctgggc cctgctgggc ggctacgagg
     2101 cctgggagcg caaggcccgg gccgtttccg gcacgccggc cgtccaggag gccggcgggg
     2161 cataatgtgg cgcatcgatt cgtgcccgtg tgggcgcgcc atttcaatca agcgtaagcg
     2221 tggagcaagt catgcggata ttggtattgg gcgccggcgg caccggcggc ttcttcggcg
     2281 gccgggcggc gcaggccggc gtggatgtga ccttcctggt gcgcccggcg cgcgcggccc
     2341 tgctgcgcga gaaggggctg cgcatcaaga ccaaggacgg cacggacgtg atccagccca
     2401 agctggtgac ggccgatgcg ctgggcgggc cctacgacgt ggtgatcctg agctgcaagg
     2461 cctacgacct ggatggcgcc atcgagtcgc tcaagcccat catgcagcaa aaccccaagg
     2521 cggcgatcct gcccatcctc aacggcatgc ggcactatga cgcgctcgac gcggccttcg
     2581 gcaaggaacg cgtactgggc ggcctgtgcc aggtcacggc caccatcggc ccggacggcg
     2641 agatcgtgat catgggccct ttcggcgcca tcatcttcgg cgaacgcgaa ggcggcttga
     2701 gcgagcgcgg cgaggccatc cgccaggcgc tggaacccgc gtccttcacc accaagctca
     2761 gcgccgacat ctaccaggat gtctgggaga aatacacctt cctctgcacc ctggccgccg
     2821 ccacctgcct gatgcgcggc agcgtgggcg agatcgtccg cgccgcgggc ggcgagcact
     2881 tcatgcgcag cctgctcatc gagatcaacg gcgtggcgca aggccatggc catggcatgc
     2941 ggcccaatgc cgaagccttt accgtgggcg cgctgaccga ccccgcctcg ggcgtgacgg
     3001 cgtcgatgtt ccgcgacctg cgccagggcg ggcagatcga gggcgagcac ctcatcggcg
     3061 acatggtgca gcgcgcggag aagctgggac tgaaagcgcc ctgcctgcgc acggcctaca
     3121 cccatatcca ggtctacctg gcgcagcgcg ccgcccaaca ggcatgagcg ccgtgccggg
     3181 tttcgagccg gtcgagcttg cgcgctcgta cctgctgctg aaccacggcc ccaccgtgct
     3241 ggtgggcagc gcgcacgcgg gccggcgcaa tgtcatggcg gcggcctggg cggatccgca
     3301 ggccttctcg ggcaaccgct ggcacttcgc cagcgatgcg cggcgttccc tgcactacgt
     3361 ggcgggcggg acgttcttcg cgacgggcga gcccttcagc gtgcctgtgg agacgtgacg
     3421 catgcgccgc atctacctgg cggggttcga cgtgttccgc gccgatgccg tggcgcaggg
     3481 cgagcggctc aaggccctgt gcttggcgca cggcttcgag gggctgtatc cgctggacaa
     3541 tgccttgccc gccggcctgg ccggccgcgc ggcggcgcaa tggatctatg agcagaacat
     3601 cggcctgatc cgccgggccg acctggtcat ggccaatctc aacgcgttcc gcggactcga
     3661 gcccgactcc ggcacggcct tcgaggtggg ctacggcgcg gcgctgggca agccggtctg
     3721 ggcctatacc ggcgatgccc gcccgttggt ggcgcaggcc ggcgcgggcc gcgacccgcg
     3781 cgacgcctcg cgcgtggtcg acgcgcaagg ctatagcgtc gaagacttcg gcctgaacgt
     3841 gaacctgatg ctggcctgcg cggcgcgcgt cgtggccggc gacgcgcagg cctgcctgcg
     3901 ggcgatggcc gcggccggct gacggcggcc gcgttcaggg gccgcgccca ggtcccacga
     3961 tcgaacgacc ccgctcaggc ggcgtgcgtc tcggcttccc tctcggcgtc cgcccccttg
     4021 gcctttgcct ggcgcaggtc ccgtagatgg tcggccagtt cggcaatatg cagcgcgacc
     4081 agcccgtgcg ccgccgcgta gcggaccagt tcggcgccgc gcatcatggt gccgtcggga
     4141 ttcatcaact cgcacagcac gccggccggg ctgagccccg ccagcgcggc caggtcgacc
     4201 gacccttcgg tatgcccgcg ccgcgccaag acgccgcccg gctgggcgcg cagcgggaac
     4261 acgtggccgg ggctgaccag gtcggtcccg gccgcggcgg gcgcgatggc ggcctggatg
     4321 gtcgtgacgc gatcggccgc cgacacgccc gtggtcacgc cctggcgagc ctcgatcgac
     4381 acggtgaacg ccgtgccgtg gcggctgccg ttgtgggcca ccatgggcgc caggcccagg
     4441 cggtcgagcg tctcgccggg caggcacagg cagacgatgc cgctgccgtc gcggatcagc
     4501 tgggccatga cgggcaccgt gagggtgtcg gcggcgacga tcaggtcggc ttcgttttcg
     4561 cggtcggcat cgtccatcag gatgatgggg cggcccaggc gcagttgctg cagcgcttgc
     4621 gcgagccgcg cggcaaaggg ctggttgaag agggaggtat tgcttgcagt aaggtgggac
     4681 atgaaacgct ctcgcggaca aggtggcgaa aaacgtttca gggctgcgga cagccgaacg
     4741 caataacacc ggcgcaagac atcgcccgag ccgggatcgt gtcggcacat cttctttcat
     4801 ccggactgtg accgtcggcc ctggcatctc accaggtctg ctgaccctgc cggcaggcag
     4861 gcgctcgcgg gctcgccgca ttgcgcggca taccgccggt ggggaatctc accccgccct
     4921 gaagacgtac tgtttggtac tggttcggcc gcgcacttcg cgcggccgag atccattgta
     4981 cgcccggctt actttttctt cttctttttg ggcagcgcca gcatggtgcc gcggcaggag
     5041 ggcgcgccgc acaggcagcg atagttttct ttcagcttct tggtgatgcg gtcgtcgagc
     5101 accaggccgt agtcgtagaa gagctcctcg ccgcgcttga tgtcgcgcag ggccacgatg
     5161 tagacgcgct tgccgcccgt gccttcctgc gattcgcagt tgggctcgca cgagtggttg
     5221 atccagcgcg cgtcgttgcc gtggtcgccg ccatcgatga tcttgcccga ggagagcgag
     5281 aagaagaagg tgtggaaggg gtcgtccggg ttggtgggat ggcgccggtc ggcctctttg
     5341 tcggaaatgc gggcgcccag gtattcgatg atgcgggtgc cttcggggat cttgcgcgcg
     5401 gcgaagatgc cgttgccgtg cagcttggag cggcgcacga cgtgccaggg cttttcgggt
     5461 tttgcggtgt cggtggtcat gggatgcgat cttagcggtt caggcagccg gcgtcacgac
     5521 cgacagcggc aggcgcgact ctttcaggct gcgcagcacg aagcccgact tgctgtggcg
     5581 gatgccgggg atgcggtaga gccgttcgcg cagcaggcgt tcgtagtcgc gcgtgtcgcg
     5641 cacggcgatg cggatgtaat agtcgtaatc gcccgacacc aggaaggcct cgagcacctc
     5701 ggggatttcg gcgagcgcgc ggccgaactc gtacagggtt tcctcgctgt ggctttcgag
     5761 cgtgacgtgc acgatgaccg tgtccatgaa gcccagggtt tccggcgcga tgtccaccgt
     5821 gtagccgcgg atcacgcctt cgcgttccat gcgcttgatg cggttccagc acggggtggc
     5881 cgacaggccc acggcggcgc cgatgtcgtt gaggctggcg cgggcgtcgt cctgcaaaac
     5941 cttgaggatg gcgagatcga acttgtcgag gtgcattttt cttataaatt gaatatatga
     6001 agattaattt acttcatata tcgggttgta gaagaaaata agacgaaaat tcttcccttt
     6061 ggccgataag atggttggca tgaacgaccg tctttccacc cctctgctgg ccgacctgga
     6121 cgcgaccgat cgcgacgatt ttccggccgg tgcgccgccc gccaacggcg cccgccgcct
     6181 gctcgatgcc ctgattgcct gcggcgtcga cacggtgttc ggctatcccg gcggcgcggt
     6241 gctgcccttg tacgacgccc tgcatgccga gccgcgcatc cgccacgtgc tggtgcgcca
     6301 cgagcaggcc gccgtgcacg cggccgaagg ctacgcgcgc accaccggca agccgggcgt
     6361 ggtgttcgtc acctccgggc cgggcatggc caacaccacg tcgggcctgc tggacgcgct
     6421 gtgcgattcg attcccgtcc tgtgcatcag cggccaggtg gccaccggcg ccatcggcac
     6481 cgatgccttc caggagtgcg atgcgctggg catctcgcgc tcggtgacca aatggaacgt
     6541 gcagatccgc gccgtagaag aagtggcgga catcgtggcc cgcgcccatg cgctgacgcg
     6601 gcagggccgg cccgggcccg tgctggtgga ctttcccaag gatgtgcagc tggccgccga
     6661 gccggcggcg cgcgaggctg gcgacccggc ggcccgggtt cgctgcctgg ccgccttgcg
     6721 cgcgcagcgc caggccggca agcgcgtcca gcgcattccg caaggcgcgg tgcagcgcgc
     6781 ggcggcgctc atcgcgcagg cgcgccgtcc ggtgttctac ggcggcggcg ggctgatcaa
     6841 tgccggcccc gaggcctgcg cggccttcac ggcgctggtg cgcgccgtgg gggcgccctg
     6901 caccctgacc ctgatgggcc tgggcgccta cccggccagc gacgcgcagt tcctgggcat
     6961 gctgggcatg catggcacgc tcgaggccaa cctggccatg caccaggccg acctggtggt
     7021 ctgcgtgggg gcgcgcttcg acgaccgcat caccggcaag ctggcggact tctgtccgca
     7081 cgcgcgcaag atccacctgg acatcgaccc ggcctcgatc aacaaggtgg tgcgggcgga
     7141 cgaggccctg gtgggcgatg caggcaagct ggcgcaggcc ctgcgggacg ccctggcgcg
     7201 gcacgatctg ccggccgcgc gcctggccgg ctggtggacg cgcatcgagg gctggcgcgc
     7261 gcggcgctgc ctggattacg cggacacgga cgacgccaac ctgccgcagc gcctgatgcg
     7321 caggctcgat gccgcgctgg ccggccgcga tgccattgtc tccaccgacg tgggccagca
     7381 ccagatgtgg gcggcgcaat acctgcgctt cgaccgcccc aaccgctggt tgacctcggg
     7441 cggcgccggc accatgggct acggcctgcc ggcggccatc ggcgcgcagg tcgcgcaccc
     7501 ggatgccgcc gtggtgtgcg tgagcggcga cgcgtcggtg ctcatgaata tccaggagct
     7561 ggccacggcc gtccagcatc gcgcgccggt caaggtggtg ttgtgcaaca acggctatat
     7621 gggcatggtg cgccagtggc aggagctgat ccacggcggg cgctacagcc acagctacaa
     7681 cgaggcgctg ccggacttcg tggccctggc ccgcgccttc ggctggcagg ccgagcgggt
     7741 gggcgacccg gcgcagctcg acgcggcgct ggcgcgttgc ctggaccatg ccggccctta
     7801 tttcctggac gtggtggtgg cgtcgcagga aaactgcttc cccatgatcc cggccggctg
     7861 cgggcacgac cgcatcatgc tggccgaggg cgtgtggcat gaagaaccgg cgtcggcggg
     7921 aagttcgtct ttcgactgag aactgggccg tatcggtcct agccggcgcg gctcaggcct
     7981 tcggcctgat catccggaac tgttccccga tggcggcata gtcggcctgg tacccggtcc
     8041 gcatcacggg ctgggccgcc caggtatcga acaggccgtc cttcaggaag gcctcctgga
     8101 tatgcacgcc gaccacctgc ccgagcgcga gccggttctc catgggccgc ccgtcgagcg
     8161 tgtgcaggcg tatcacttgc agcagcttgc attcgagcgc ggccggcgct tgcccgacat
     8221 gcggcaccga gacctggcgg cccgcggccg gcgtgagtcc ggccagcgcg aattcgtcga
     8281 cctcgggcgc gaccggcgcc gaggagcggt tcatctgctc ggccagggcg cgcgaggtga
     8341 agttccagac gaactcgccg gtttcctcga tattgcggat gctgtctttg gtgccctcgc
     8401 tggaaaagcc aatgatcgga ggcgaggtcg agaagatgcc gaagaagcta tagggcgcca
     8461 ggttcaggac gccttgcgcg ctgcgggtcg aaatccagcc gatcacgcgt ggcgcgacga
     8521 tggccttgag cggatcgtgg ggcaggccgt ggccttgggc ggggtcgtag aagtagctgc
     8581 tcatcgtcgt aatggggtcc tgtgccatga aataaggttg tcattcatgg ggcgcagact
     8641 aagcgtatca gttgaaaacg ttttcaattt atacaccccc ttgtcgattt cccgcgccgc
     8701 cgtgacacgt aaagccgcca gccgttcgtc catcgtcgaa gtcgcccgca aggcgggcgt
     8761 ctcgcccgcg accgtgtcgc gcgcgttcaa ccagcccgac ctgctcaggc cggccacgct
     8821 ggcgcgcatc gaggcggtgg cgcgcagcga cggctttcgc cccaaccgcg tgggccgcag
     8881 cctgcgcgcc ggcagcacgc gcaccatcgg cctgctgctg cccacgctgg gcaatccggt
     8941 cttcgccgaa tgtttcgaag gcgccgaaca gcacgcgcgc caggccggct acagcgtcat
     9001 gatggcgacc accggctatg cgcccgaggt cgaggtcacg gcggtgcaga ccttcatcga
     9061 ccaccaggtc gacgggctca tcctcaccgt cagcgcgccg gcgcgcaaca ccgcgctggc
     9121 gatgctgcag gcggccgagc tgccgcatgt gctggcctat aacgaatccg gcacgcaccc
     9181 cttcgtctcg gtcgacaacc atgccgccgc gcgcgacatg atgcaggccc tggccgcgct
     9241 gggccatcgg cgcgtcgcca tggtcaccgg cccgctgtcc gcctccgacc gggcgcgccg
     9301 gcgcctggac ggcgcgcgcg tccaggccgg ggcgctgggc ctggacgagg tcgcgcacct
     9361 ggtcatgccg gcgcataccg cggccagcct cgaattgctg caggccgcgc tgcgcggccc
     9421 ggccgcgccc acggcgctgt tctgttccaa cgacctgctg gccgcctcgg tgattacgca
     9481 cctggtggcc atggggctgt ccgtgccgcg cgacctctcg gtgtgcggct tcgacggcat
     9541 ggccatcggc gcgctgatgg tgccgccgct gacttccgtg gagcagccca gccgccagat
     9601 cggcgccacg gcctgcgccc atttgctgac ccgcctgctg gggggccaaa ccagttcggt
     9661 gcgtctgccg caccggattc tctcgggagg cacggttgcc gcgccgcccc tatccactcg
     9721 taccgcgagg agaaaaaccg catgaaacgc atgatcaaca ccgcattgcg ctgcgcgttg
     9781 gccgccctgg ccctcggcgg cggcgccgcc caggccgccg aaacggccat ttgctacaac
     9841 tgcccgccgg aatggggcga ctgggctggc cagatcaagg ccatcaagga cgccaccggc
     9901 atcgtcgttc cgcccgacaa caagaactcg ggccaggccc tggccgcgat cgccgccgaa
     9961 aaaggcaacc ccgtggccga cgtgacctac ctgggcgtga cctttggcta ccaggccgcc
    10021 aaggacggcc tggtgcagtc gtacaagccg gcgcattggg acgacattcc cgccggcctg
    10081 aaggatcccg acggcaagtg gttcgccatt cactcgggca ccatgggctt catggtcaat
    10141 gtggacgcgc tgcacggcaa gccggtgccc aagtcgtggg ccgacctgct caagcccgag
    10201 tacaagggcc tggtcggcta cctggatccg gcctcggcct tcgtgggcta tgtgggcgcc
    10261 gtggcggtca accgcgccct gggcggcgcg ctggacaact tcggtcccgg catcgagtgg
    10321 ttcaagaagc tgcaggccaa ccagcccatc gtccccaagc agacggccta tgcccgcgtg
    10381 atctcgggcg agatccccat cctgctggac tatgacttcg acgcctaccg cgccaagtac
    10441 accgacggcg ccaacatcga cttcgtcatc ccttccgagg gcacgatcac cgtgccttac
    10501 gtgatgagca tggtggccaa cgccccgcac gccgccaacg cccacaaggt gctggatttc
    10561 gtgctgtcgg acaagggcca ggcgctgtgg gccaatgcct atctgcgtcc ggtgcgcgcc
    10621 agcgcgatgt cgcccgaagc gcagaagaag ttcctgcccg acagcgaata cgcccgcgcc
    10681 ggcacggtgg actacctgca gatggccaac gcccagcgcg ccttctcgga cctctacacc
    10741 aaggaagtcc actgacatgg ttcatcgccg cggatggctg gttttcgccg cgccggccgc
    10801 ggcgctgttc ctggcttttt ggctgtttcc catggccggg ctggcgctac gcggcgccag
    10861 ccccgtcgat gggcgttcgg cctacctgat cgtggtgacc gagccgcgct actggcgcag
    10921 ccttttcaac acggccgccc tgtcgctggc ggcgacactg ggcaccttgc tggtttccct
    10981 gccggtcggg cgcttcctgg cctggcatcg cgactttccc ggccgccgca tcctggtggg
    11041 actgctgatg tttcccctgg ctttccccgg cgtggtggtg ggctttctga tcatcctgct
    11101 ggccggccgc caaggggtgg tgggccagtt cacgcagtgg ctgacgggcg agcccctggt
    11161 gttcgcctac aacatgggcg gcctgttcct gggctatctg tatttttcat tgccgcgcac
    11221 catcggcgtg atcgaggcgg ccgcctcgca gatggacgcc agcctgctcg aggcggcccg
    11281 cacgctgggc gcctcggcct ggcggcgctt cgtcgacatc gagctgccgg cgctccggcc
    11341 cgcgctgatc ggggcgggcg cgatgggctt cgccaccaat atgggcgcct tcggcacggt
    11401 gttcacgctg ggcacgcgca tcgacgtgct gcccatgacc atctacaacg agttcaccaa
    11461 ctacgccaac atcccggtgg cggcggcgct atcgatcctg ctgggcctgg tcacttgggt
    11521 ggcgctgtac atcgcgcaca gcgtggccgg ttcgagcgcg ggggcgggcg catgatgcgt
    11581 gcctcccgcc tcgcgctacg tgttcggcga ccccccaagg gggcaccatg agtaccttgc
    11641 ataccaaggc cgacgcccgc ctgggcctgg ccgtgacgct ggccgtggcg gcttttctca
    11701 tcggccccgt ggtgctgtcg atactggccg gcctgaccaa gaactacttc gtcggcattg
    11761 ccagcggcct cacgctgcgc tgggtggcgc aggtctggga cctgtacgcc gacaccatct
    11821 ggcgttcgct gttcgtggcc ctgttgacgc tcgccatctg cctgctggcc ggcgtgccgg
    11881 ccgcctgggt gctgacgctg taccgcggcc gcctggcgcg cgcgctcgaa gaactgctga
    11941 ccctgccggt ggccgtgccg ggcctggcct cggcgctggc gctgatcgtg tcctggggca
    12001 cggtgacggg cctgcgcacc agctccctgt tcatcgtcat cggccatgtg ctgttcaccc
    12061 tgcctttcat ggtgcggtcc acgcgcgcca gcatggcggg cgcgggcctg gcggtgctgg
    12121 acgaggcggc cgccacgctg ggcgcttcgc ggctcacgcg cttcctgacc gtggtggtgc
    12181 ccaatgcccg ggccggcatc ctcaccggcg gcttgacggt gctgacgctg tccatcggcg
    12241 aattcaacct gacctggctg ctgcacacgc cgcttaccca gaccctgccc gtgggcctgg
    12301 ccgacagcta cgcctcgatg cggctggaag tggcttcggc ctacaccctg gttttcctgc
    12361 tgatgctcat gccgctgctg gtgggcatgc agtggctggc cgggcagggc acgcgccgcg
    12421 cggccggcgg caagggcgct tgaagacacg tgcctgaaga cgaacccggc cgaccggccc
    12481 gaccacgcca tcccaacatg aacaagactt ccatcaccct gcgcgaatgc gccaagacct
    12541 ggccggacgg cacgcgggcc ctgcagccgc tggacctgcg cgtcgagggc ggcgaaatcc
    12601 tcgccctgct gggcccctcg ggctgcggca agaccacctt gctgcgcctg atctgcggcc
    12661 tggaaaccag cgacgccggc ggcaagatct attacggcga cgacgacatc acgcacttgt
    12721 cgcccgagcg gcgcggcgcc ggcgtggtgt tccagagcta tgcgctcttt cccaatatga
    12781 gcgtggccga caacgtggcc tacggcctgc gcgtgcgcgg ccaggacgcc gcgcgccgca
    12841 agcagcgcgc cctggacatg ctggcgctgg tgcgcatgca ggactacgcc gatcgcaatg
    12901 tgacgcagct ctcgggcggc cagcgccagc gcgtggccct ggcccgggcc ctggccatcg
    12961 agccgcgcgt gctgctgctc gacgagccgc tgaccgcgct ggatgccaag ctgcgcgagc
    13021 acctgcgcgt ggagctggcg cagatgctgc gcgagctggg cgtcaccacc gtcatcgtca
    13081 cgcacgacca ggacgaggcc atgatgctgg gcgaccgcat cgccgtgatg tcggccggcc
    13141 gaatcgagca gatcggcacg gccgaaacgc tgtaccgcca gccggccacg gacttcgtgg
    13201 gcgagttcct gggcacgctg tgccacgtgc gcgccgcgac cgcggacggc ctgctggtgc
    13261 cgggcgcgag cgagctggcc ttccgcccgc accacgccgc gctgctggcg ccgcagcccg
    13321 agtccctgca ggcccgcgtc gcggcgcgat ttttcctggg cggcacggtg cgctacgagc
    13381 ttgccttgcc cgacggccag cggttctccg ccatggcgcc cgccgacagc gcccaccagg
    13441 ccggcgatgc ggtcagcatc aagctggtcc ggtcttaaac agattccctg caaccattca
    13501 ggacgcaccc atgctcatcg cccagatttc cgacctgcac atccgcatgc ccgggcaaaa
    13561 ggcctaccgg gtggtcgaga ccgaccgcta cctgccgccc gccgtagagg ccctcaatgc
    13621 cctggacccg ctgcccgacc tggcgatcct cagcggcgac ctcaccgatt tcggccggca
    13681 tgtcgaatac gcgcacctgc gccggatgct cgacggcctg cgcatgccct actacctgct
    13741 ggccggcaac cacgacgagc gcctgcagct cgcgcaatcg ttcgaccacc tgccctatct
    13801 gcaaggaacc ggcgagttcc tgcagtacac ggtcgaagat catcccgtgc gcttcatcat
    13861 gctcgacacc gtggtgccca aggccagcca cggcgagctg tgcgggcgcc gcctggcctg
    13921 gctggccgac cgcctggccg agcagcccgg ccggccgacc atcgtggcca tgcaccatcc
    13981 gcctttcgcc acgggcatcg cccacatgga cgaaatcggc ctgctgcgcg gcgcgccggc
    14041 cttcaaagcg ctggtggcgc gctatcccaa tatcgagcgc attctgtgcg gccacctgca
    14101 tcgcaccatt ttccgccgct tcgccggcac ggtggtgtcc acctgtccga gccccgcgca
    14161 ccaggtggtg ctggacctgc ggccccaggg gccctcggcc ttcatcatgg agccgccggg
    14221 cttccagctg caccactggt gcgacggcgc gctcaacacg caccatgcct acatcggcga
    14281 ctatcccggc ccctatccct tccacgaaga cggcatgctg atcgacgagt aattaagctg
    14341 attgaaagac tgacatgcgg cggtcatctt gggtctgcag aatgaaaagg ttttcattcg
    14401 acggtcccaa gcttccttga gacccggtcc ttcccatgct cgtcctggaa aatctcggca
    14461 aacagtacgc cggcaatgtc gcggtcgatg gcgtgaacct gcacgcgccg gccggcagct
    14521 tcacggcctt gctggggcct tcgggctgcg gcaaatcgac gaccctgcgc atgatcgccg
    14581 gcctggacgc gcccaccgcg ggccgcatcc tgatcggcgg gcgcgacgtc acgcagcttg
    14641 cgcccgcgca gcggcgcatc gccatggtgt tccagtcgta tgcgctgttt ccgcacctgt
    14701 cggtgcgcga gaacatcctg ttcggcctgc gcgtgcgcaa ggagcccgag cgcgatttcg
    14761 accgccgcct gtcgcgcgtg gcgggcctgc tgggcctgtc ggccctgctc gaccgcaggc
    14821 ccgcgcagct ctcgggcgga cagcagcagc gcgtggcgct gggacgcgcg gtgatttccg
    14881 aggcgcccgt ctgcctgatg gacgagccgc tttccaatct cgacgcccaa ttgcgccagg
    14941 acatgcgccg cgagatccgc ggcctgcagc aggcgctggg catcaccatg gtgtacgtca
    15001 cccacgacca gaccgaggcc atgagcatgg ccgaccaggt cgtgctgctc aacgccggcc
    15061 gcatcgaaca gcagggcacg ccgcaagcgc tctacgcgcg gccggccacg cccttcgcgg
    15121 cgcgcttcat cggcacgccg cccatgaacc tgatcgccat cgagcccttg ttcgggcagt
    15181 ccgtcatccg cggcacgcac ggcccggcga tcgcgggcgc gcctggccat gcgcggctgc
    15241 tgggcctgcg gcccgagcac ttgcggctcg acgcgtcggg cgtgccggcc gagatcgagt
    15301 ccgtggagta tttcggcgcc gattccatcg ccctgtgccg cgtgggcgac ggccgcatcg
    15361 ccgtgcgcgt gggcggccac ctgcgcgccg agcgcggcac gcgcacgggc ctggcctgga
    15421 accccgggca ccagcacttt ttttctgccg atcccgccga cacggcggct gcatgacccc
    15481 tgcttcaaca ctcaacccag aggaagacaa cacatgcaac gcattgtcct gaaaaccttg
    15541 gcggccagcc tggccggcgc ctttttcgcc ctgcccgcgt acgccgcgac gcaagtggag
    15601 ttctactacc cggtcgccgt gggcggcccc atcaccaaga tcgtcgatga cctggcggcc
    15661 cagttcaaca aggaaaatcc cgacatcgtc atcaagccgg tctactcggg cacgtaccag
    15721 gacacgatcg ccaaggccct gaccgcgctc aagggcggca cgccgccgca attggccgtg
    15781 ctgctgtcga ccgacatgtt cacgctgatc gacgaggacg ccatcgcgcc catcgacagc
    15841 ctggcgcagt ccgacgacga caaaaaatgg ctgggcggct tctacgacgc cttcatggcg
    15901 aacacccgcg ccaacggcca cacctggggc gtgcccttcc agcgctcgac catcgtggcc
    15961 tactacaaca aggatctctt caaggaagcc ggcctcgacc ccgagcaggg tcccgccacc
    16021 tgggccgaac tggtcgacga cgccaagaag ctcaccaaga aagacgcctc gggcaatgtc
    16081 acgcagtggg gcgtggaaat tccctcgagc ggcggctatg gctactggat gctccaggcg
    16141 ctggccacgc ccaacgacgc gctgctgatg aaccaggctg gcaccgagac ctacctgaac
    16201 aagcccgccg tggtcgaagc cgcccagtac tggtacgacc tggcctacaa gcagggtgtg
    16261 atgccgaagg gcaccaccga atgggccacc atccccaagg atttcctgga aaagaaggcc
    16321 gccatcgtct ggacgaccac gggcaacctg accaacatcg ccaccaatgc cagcttcccc
    16381 ttcggcgtgg cgatgatgcc caagaacaag cgcggcggca gtcccacggg cggcggcaac
    16441 ttctacgtct tcaagcaggg cacgcccgcg cagcagcagg ccgcgctgaa gttcgccaag
    16501 tgggccactt cgcccgagca cgccgccgac tggggcatcg ccaccggcta cgtagccgtg
    16561 acgccggccg cctgggaaac gcccaagatg caggcctacg tccagaagct gcccgccgcc
    16621 gccgtggcgc gcgaccagct ggccgtgagc gtcgccgagt tctccacgca cgacaaccag
    16681 cgcgtgacca aggtggtcaa cgacgcgatg caggcgatca tgaccgacgc caagaagccc
    16741 cggcaggcgc tggacgacgc gcagaaggaa gccggccgcc tgctcaagtc ctaccagtag
    16801 acggcggcca cggggacgcg agcgtatgaa ccatcgcaga ctcgacgcgg tgtacggctg
    16861 gctgatgctg ctgccggccg tcgtgctgct cgcggccttc acgcattacc cggctgtcgc
    16921 caccgtgtgg gacagcctgt tctccacccc caagggcgtc cgcccggcgc atttcgtggg
    16981 gctggacaac tacctcggca tgttcgatga cgacgtgttc tggcaggcct ggtggaacaa
    17041 cctggcctac gccgccgtca cggtcccggt ttccattgcc gtggccttga tcatggcgct
    17101 gtgggtcaac ggcaggctgc gcggacgcgg cttcctgcgc atggcttatt tcacgcccac
    17161 ggtcctgccc atggtggccg tggccaatat ctggctgttc ttctataccc cgcaatacgg
    17221 cttgatcgcg caggtcatgc atgggttcgg cctgcccggc tacaactggc tgggctcgcg
    17281 cggcacggcc ttgccggcct tgatgctggt ggcggtgtgg aaagaatcgg gtttcttcat
    17341 gatcttctac ctggcggcgc tgcagaccct ctcgccgtcg ctgcgcgagg cggcgctgct
    17401 cgaaggcgcc tcgcgctggc agtatttccg gcgcgtgctg tggccgatgc tcatgcctac
    17461 caccctgttc gtgctggtca atgccgtcat caacgctttc cggctgatcg accacgtggt
    17521 ggtgatgacg cacggcggcc ccgacaacgc cagcatgctg ctgctctact acatctacca
    17581 ggtgggtttc agcttctggg acacggccta cgccgccacg ctcaccgtgg tgctgctgac
    17641 ggtgctggcc gtcatggcgc tggtgaagtt tcgctggttc gaccgcaaga cgcactacca
    17701 atagccatga cccgcaagct cgatacgata ggcgcctggt tgctgggcct gctgtggatc
    17761 ctgcccctgg cctatgccgc ctgggcggcc ttccacccgc ccgcctacgc cacgcggttc
    17821 gagctgctgg cgccgctgac gctggagaac ttcaagcgcg cctgggcggc cgcgcccttc
    17881 gcccgctact tcctcaacac cttcatgctg gtgaccatgg tgctggccgc gcagttcgtg
    17941 ttgtccacgc tggcgggcta cgcattcgtg cgcttcaatt tccgcgggcg cgacttcctg
    18001 ttcctgatgg tgctgctgca gctgatggtc atgcccgaca tcctgctggt cgagaactac
    18061 cgcaccatga gctggctggg cgtgcgcgac acggtgttcg ccatcggcct gccgtatttc
    18121 gcctcgggct tcggcatctt cctgctgcgc cagaccttca agacggtgcc gcgcgagctg
    18181 gaagaggcgg cgcgggtcga gggggcgaac cccctgcagg tattgatgaa ggtgtatgtg
    18241 ccgctggcca agccgactta cgtggcctat ggcctggtgt cggtgagcac gcactggaac
    18301 aacttcctgt ggccgctcat catcaccaat tcggtgggcg cgcggccgct gacggtgggg
    18361 ctgcaggtgt tctcgggaac cgaccagggg gtggactggt cggtgatcac cgccgcgacg
    18421 ctgctgtcgg ccgggccctt gctggtcgcg ttcttgctgt ttcagcggca gttcgtgcag
    18481 tcgttcatgc gggcgggaat acgctgagta cggaatctca gaatccgacg gcgtccctgc
    18541 cttttagcgc gagcatcttg cgggcttcgt cgggcgaggc aatttcaagc ccgagcccct
    18601 cgatgatctg gcgcgcagcg cggacctgct gtgcattcga ctcggccaat cgcccgcgtc
    18661 cgatccacag cgaatcttcc agccccactc tgacattgct gcccatcgct gccgacatcg
    18721 ccgcaatggg caactgactg cgtccggcgc cgagcacgga ccagcgataa tcgtcgccga
    18781 aaagccggtc ggcggtgcgc ttcatgtggg cgacatcctc cggatgggtg ccgatgcctc
    18841 cgagcagccc gaagacgctt tggacgaaga ggggcggctt gatgatgccg cgctcgacga
    18901 aatgcgccag cgtgtagaga tggccgatgt cgtagcactc tacttcgaag cgcgtgccgt
    18961 tctccccgca ggtcgtcagg atgtactcga tatcagtgaa ggtattgcgg aagatgcggt
    19021 ctttggagcc ttccagatag ggtttttccc agtcgtactt gaactcgttg tggcgggcca
    19081 gcatcggata gagcccgaaa ttcatcgaac ccatgttgag gctcgcgacc tccggcttga
    19141 aatgcgccgc gggacgcacg cgctcctgca ccagcatggt gggagcgccg ccggtcgtga
    19201 tgttgatcac gcagttcgag cgctgcttga tgtccttgag gaatggagca aaggcctccg
    19261 ggctctggtc gggcttgccg gtctcggggt cgcgcgcgtg cagatgcacg attgccgcgc
    19321 cggcctcggc ggcctcgacc gcggcatccg cgatttcctg tgctgtgatc ggcagatgtg
    19381 gcgacatggt cggggtgtgg atcgctccgg tgacggcgca ggtgatgatg accttccggg
    19441 ggggatgtgg catggcgtgt acctcattag gttatggccg atcctaatcg gcagtcggtt
    19501 tcagatgccg aggctggcaa gaagggcgag gatggtccgc gcgttcgcga gggtatccgg
    19561 caggaatgct gtatcgaatt cggcgagggc atcacccgcc ggcaggcaac gcaacgtggc
    19621 ggccgaccgg cgcatagggg gacgaagacg acgcccagtc cgtggcagat gccgggaatg
    19681 tcgtttcccg catgccgtac ccaggacgag tctttcgtct gtggcactgt cgccggatgg
    19741 agggcggtat gggtgtgttt tagggttcca ggaaggcgtc cccaataaat agatataacg
    19801 aatagtgcct tctgcggctg gccgagggtt ggcatggcat gccagccgta catattcggg
    19861 cttgctgttg acggaaggcg cgtcgtcttc cgccttgagg tttccgaatt ttctcgcgat
    19921 gacgaagccg tttctgtttc tggaaactgc cttggacgtc ggggcgtcgt tgccgtatgc
    19981 gtccggcttg tcgagtgggt ttgcgccgat ttccgcgggg tttggcacgt cgtggcgcga
    20041 cgtcatgtcc ctgcggccaa caaaccccag gcacgaaacc gggggctctc ctggattgtg
    20101 cgtctccggc atatcctcgc cttttttatg gtgatatgaa aagtgatata acgatcttgt
    20161 aacactccct tgacatggtg gcaagcggag ttcatgatgc ctgcaaaatg aggttgtagc
    20221 gccgaattct cggccttaga agaatggggt tccaagtttg aaagaagacg atatatcagc
    20281 tcccgctgac gccgaagatg agggcgggca ccgcgccgcc atcctgcatg acgacatcta
    20341 ctcgacgctg cgtcacgact tggtgacggg ggggttggtg ccagggcagc gtgtctcgat
    20401 ccgtactttg gcggaacgct tcgaaaccag ctttcttccc gtgcgggacg cgctcaagcg
    20461 gttggtggcg gaacatgccc tggtcatgct gcccaatagg acggttctcg tgccgatgat
    20521 gacgcgtgaa cgctttcagg agttgctgca ggtgcgcctg atgctcgaaa ccatgcttag
    20581 ccgccaggcc gccgcgcgta tcgttcatga cgaggtcgtc aagctcgacg cgatcaacga
    20641 ggcaatgcag caggcagtcc gcgacggtga tgtgcgccgc tatcttgccg ccaaccacgc
    20701 gttccatttc ggtctctacg aggcgggggg aagtgaagtc gccctgccga tcgtcgacaa
    20761 actctgggtt caggtgggcc ccttcctgaa cgccgtcttc aaccagcagg ggacccggag
    20821 cgccggcggc aatcacgccg aagtgctgaa ggcgctacgc cgacacgacc ccatcggcgt
    20881 tgccgaggcg attcatagcg atctcgccaa tgccgccgac ctgattctgg cgaacgtcga
    20941 gttccccgaa tgggcatacg aacccaagcc ccgacgcatg aaacatccga atgggggcgc
    21001 gcatgaacag gaggcgctct cgcaagacgg accgaaggcc gcggtaagcc ccgatagaag
    21061 ggcaagcggc cgaaaagcga accaagaata aataaaacga atcaaaaaaa taccaggagg
    21121 agacaaaatg accaagaaac ccatcatgcc ggccgtgctg gccgccctga cggccgcggc
    21181 ctcgctggcc tggcctggaa ccggccgagc cgagccgggc gtgaccgaca cggaaatcac
    21241 tatcgggatg ttcgcgccgc tctcggggcc cctcgtcgcc ttcggtatcg atccattggc
    21301 ggccgccaaa atgctctacg aggaggtcaa caaggcgggc ggcatcaacg gccgcaagat
    21361 caagacgctg gtcgaggacg acaagtgcaa tccgaacgac cttgttgcgg tagtcaagaa
    21421 gttcgtcacc gtggacaaag tgttcctgct gcacggcggg tcctgcacgg cggccatcag
    21481 tgcggcttcg gactacatca accgcgagaa ggtgcccttc gcgatgctca acgcggctgg
    21541 cgacaactcg gtctttccgc ccacacgcta cgtgttcggt tccttccagg gaacgcagcg
    21601 tacgtattcc gccgtattcg cccgtttcgc cggtgaatac ctgaaggcca agcgcgccgc
    21661 gatcatcgtg cacgacgacg agtacggcaa ggcgaacgcc tccatggcca aggcgacctt
    21721 cgacaagatt ggcgtcgaga cggtcgacat cgagcgcatc gcaccggaca tctccgacgt
    21781 gaccgcgccc atgctgaaga tccgcgcggc caagcctgac gtcattcttt ccggcgtcta
    21841 tcccgcgccg gccgtgctgg tcgcccagaa atacggcgag tttggcatga cgggcatccc
    21901 gctgctccag gcggcgcagg gcattccaac gccgtcggtc ttcgccaaga acgtcgcgaa
    21961 cccggcggcg ctgcagaact tctattacac ctgggctttc aatgacatcg gcgatgccgc
    22021 agtccgggag aagtacatca acatgttcaa ggcctacaac aatggccgcg agcccggccc
    22081 gttcatgacg agcggcctgc cttcggccat ggcggtcatc gaggccttga aggccgcggg
    22141 ccgcgacctg acgcgcgagg gcttcatcac ggcgatggag aagctcgatt atcagagcga
    22201 catcatgccc gggccgctgc agttcggccc gaaccgccgc gacgcggcgc ggacgatgcg
    22261 gatcatcaaa tacgacggcg tgacccagca agtcatgccg ggctccttca gctgggacgg
    22321 caagcagccg acggagtgac cgccggattc gaggtcgcac accacttcac tcgaccgagg
    22381 aaggaagagc catggttatg caggcaatcc tgagcggcat cgtgatcggc tgcgtttacg
    22441 cactgatcgc gctctcgctc gtcatcatct acaaatcgac cgacgtggtg aacttcgccg
    22501 ccggcgagtt cgtcatggtt gcggcctatg ccgggctgct gctgctcgat cattacaaga
    22561 tcaactacgc ggtcatgacc gtgctggccg tcgggctcgc cttcgtcttg ggcgggttgt
    22621 tcgagcgcgt ggcgctcagc cgcatcatcg gcttcaaata tgcgaccgag gccgcgctgg
    22681 tgccgatggt agtggccacg atcggcctgt ccttcatgct caagggcgcc gtgcgggtga
    22741 tcccctcgac ccaggaggtc ctgcgcttgc cgccgatctt ctccggaccg ccggtctttt
    22801 tcggcgatct cgtcctgcag cgccaggata tcggcatcgt gggcgtggcg gtgctggtga
    22861 tgatcctcct ctcgctcggc ttcaacctga ccgcgaccgg caaggcgctg cgcgccacca
    22921 gccagaactc ccgcgccgcg gcgctgatcg gcataccggt caaggggatg cgtacgatgg
    22981 cttgggctgt ggccgcgggg ctcgccggca tttcaggcat cctgctcgcg cccaagctgc
    23041 tcatgacgcc cgatatgggt tcggtattca tgatgggcat cgccgcgtcc atcgtcggcg
    23101 gcttcacgtc gctcgtgggg tgcgtgattg gcggcatcct gctcggcgtc ctgcagaatc
    23161 tggtcggcat cttcgtctcg ccgcaagcga tcaccgtcac cccgtttttc atcatcatgc
    23221 tcgtgctcgt cgtccggccc aacggcctgt tcggcgaagc gatgcggcta aaaaaggtct
    23281 gacatgtacg gttcgcctcg ctatccggaa ctggccggac tcctcgtcat cgtcgccgtg
    23341 ggcttcgtgc tgccgtggct gttgcccgag tacttcctct atacgtgcaa tatgctgatg
    23401 acctacgcga tactggccat cggcctggac ctgctgcttg gctggtcggg acagtttgcc
    23461 ttcgcgcaca tcgccttctt cggtatcggc atctacggta ccgcgctgct cgaaagccgc
    23521 ctcggcgtgc cttacgtgtt gagcgtggcg gcttccgccg gccttgccgg cctgctcggc
    23581 atcgtcatcg gcctgccggc gacccggctg cgcatggttt atctggcgct cgccacgtat
    23641 gccttcgctg aatgcgcgca gtgggtgttc cgatcctggg atcaggtcac tggcggcagc
    23701 gacgggctgc gttttccccc gccagacatc ttcggctaca caaccggcac cgacagccgc
    23761 gcttttcccg tggtcgcgct gatgctcgcc ctggtgttgg gcgcagcgct ctatctgacg
    23821 agatcctcgc tcggccggca gatgtgcgcc atccgcgaca gcgagcacgt cgccgcggcg
    23881 tcgggcatcg acgtgcggcg catcaaggtg atcgctttcg ccatctccgc ggtcttcgcc
    23941 gggatcgcgg ggggcaccta cacgctgttc caatccttcg tcaacgcgga cgtgctgggc
    24001 gcatcgcagt tggtgacggt gctcaccatg attgtcattg gcggctccgg ctcgatcgtc
    24061 ggcgtgctcc tgggcgtggc ggtgattggc ctcctgcccg agctcttgag gatcatgccg
    24121 cagagcatgc tggtctggca ggagttcatc tacggcgcca tcctggtcct gtcggtggtc
    24181 ggcatgccca agggcctgtg ggggctgatc aagccgcggt tcggtaaaaa gagcaaggaa
    24241 accaagactg cgcctgtccc gccgacgcac tctaccttca cggagattgc ggaatgagcg
    24301 cttttctctc cctggacgac atcagcgtgc gcttcggcgg actcaaggcg gtcgaccgtt
    24361 tctccatgga agtccaggaa ggctccatcc atgcgctgat tggcccgaac ggtgctggca
    24421 agtcgacggt gttcaactgc atttcccgat tctgcacgcc gagcgaggga ggcatccatt
    24481 tcgacggtcg ggacctgctc tcgctgccgg cacaccgcgt ggtctcgtcg ggcatcggtc
    24541 gtacctttca gaacctcgaa ctgctcaagc ggatgactgt gctcgagaat gtactgattg
    24601 gccaaaccgc gcatgtgccg cgctatgtct cgttcgggcc gggcgcgaag cgcaagagga
    24661 ccgaggcgca ggccgtgcgc gaggccgagg cggtgctgga ggaaaccaac ctcctgcctt
    24721 atcgcgacac gctcgccggg gagctggatt tcggtcatca gaaactcctg gaccttgccc
    24781 gcgcgctcgc cggcaagccg cgcctgctgc tgcttgacga gccggccgcg gggctgcgaa
    24841 accgcgagat ttcctttgtc gatgagttgc tggtcaagct cgcccattcg cggcggttga
    24901 cgatcattct cgtcgagcat gtcatgccgc tggtcatgtc gattgccgac cggatcacgg
    24961 tactcaattt cggccagaag atcgctgaag gcgcgccggc ggagatacgc tgccatccca
    25021 aggtgatcga ggcctatctc gggagcgacg cccatgctta ggctgtccaa catcgaggcg
    25081 ttttacggat cggtccaggc gctcgacaac gtttccctgg aggtcggcga cagtcaaatc
    25141 gtctgtctgc tcggcgccaa cggcgccggc aagaccacga tgctcaactg cgtgtccggg
    25201 ctggtggctg cgccgcgcgg caagataacc tttgacgggg aagacatcac caatatcgga
    25261 gcggaaggcg ttgtggccag aggcatcgtg caggtgccgg aaggccgcga gatcttcccg
    25321 gcgatgaacg tggaagacaa cctcgtgctg ggcgcctggc agaaaaagca aagcaggcgc
    25381 cacacgcgcg agcagctcga gcgcatttac ggcttgttcc cgcgcctgcg cgagcgcgcc
    25441 cggcaacatg ccggaacctt gtccggcggc gagcagcaga tgctgatgat cggccgcgcg
    25501 ctgctcgccg agcccaagct gatcatgttc gacgagccca gcctcggcct ttcgccgctc
    25561 ctcgtgcagc aggtcttcgg catcatccgg aagatccacg aacagggaat gccgattctg
    25621 ctcgtcgagc agaacgcccg gctggccctc aaggtcagtt cctattgcta cgtgatcgag
    25681 aacggcgaga tccgctttca cggtccgagc caagcgctca tggaaaatga ggaaatcagc
    25741 aaagcctatc tcggctaaca gaaaggagga gaccagacat gaccaagcta tccgaataca
    25801 aagacaaata cgaatgcatc aagttcgagc ggcgcgacga catcttgcag atgacggtgc
    25861 acaccaacgg tgattcgctt cgctgggggt tggtgccgca ccgcgagctg ccgatggctt
    25921 ttttcgacgt cgccaacgac tatgagaaca aggtggtgat ccttaccggg accggcgccg
    25981 agttcagcgg cccgcaggtc atcccgtccg acggccatcc gatattcccg actcgtccga
    26041 agatggagtt ggtcgatgcc ctgctctcgg agggcaagca aggactgctg aacttcctgc
    26101 agattgacgt gccggtgatt accgccttca acgggccggc ttggcgccac ggcgaaattc
    26161 cgcttatggc cgacatcgtc atcgcggccg ataccatcgc cttccaggac tcggcgcatt
    26221 tccagggcgg tctcgttccg ggcgacggcg tgcatgtcgt ctatcccctg ctgcttggcg
    26281 tcaatcgggc gcgctatttc ctgctgatgg gtcagatcct gcgcgccaag gaggcgctcg
    26341 aatacggcct ggtctccgag gtattgccgc ccgacagggt gctgccacgc gcctgggaga
    26401 tcgccaggaa tatggcctcg aagccgaaga gcctgctacg cgcttcgcgc gccgtgctga
    26461 ccgagaaact caagcgggac gcccaggaca tgatttcctt cgggctttat gccgaactcc
    26521 tggctctcca ggacaaaccc gacgcaatct aacgaggaga cccacacatg ctgttcgctt
    26581 tgaaaatcga agtttccgtt cccgccgacg tcccgcagga gaccaaggcg gacctgcgca
    26641 agcgggaaaa cgcgcgggcc atcgagctca tcaagcaggg ccggctgaag cgcatcttcc
    26701 gcgtggtcgg cgaggtgtcg aacatcagcc tctgggaggc cgagacgctg gagcagttgc
    26761 atgccgacgt cggctcgctg ccgctctacc cctacatgaa ggtgcacgtg acgccgctca
    26821 tcgagcatcc ggtgaccgcc gcctggaaag agacgaacgg cgccctgccg ccattttgaa
    26881 cgaacgcgcc cggcccgcgg ccggcgcaac gcatcatagg agacgatcat gagcaccaac
    26941 agcatcaccg ttttcaaggg cgacacctct cgtctcaaga ggatgatcac cgtccccgac
    27001 agaatgagcc cggaaggcta tcgactcgaa ctggagaaga tcttcagccg ggcgtggctg
    27061 ccgaccctca gcgtatcgga tgttcccgag aacggctcgt ataccgtcgt cgacgtgccg
    27121 ccgaagaaga cctcggtgat cgtcgtgcgc ggtaacgacg gtgtcgtccg cgcttttcag
    27181 aacgtctgcc ggcaccgcgg tgacatgctg gtccacaaag gtaacggtaa gacgaagcag
    27241 ttcgtttgcg gcttccacgg ttggcgctac tcgaccgagg gtgcgctggc cggcgtgacc
    27301 gacgagacgc agtttccgga catcgagcgc gacaagctcg gcctggtccc ggtcaattgc
    27361 gaggtctgga acgacctggt cttcctgaac ttcgagaaga cgccgcacgt cacgctcaag
    27421 gaatggctcg gcggcatctg ggatgaatac agcggctatg ccgaaggccg ggagaaggtc
    27481 gccgaccatc gcgttgtgct caaggccaac tggaacgtcg ccgtgaatgc cttctgcgag
    27541 ggctatcaca acatgtacat ccacaagtac acggtgcccg actaccaggg cggcaagggt
    27601 aatccgctgc gccatcgcgc ctatatcgag atcggcaagc gcttcggccg ctattcggcg
    27661 cacgccaata tggaccacaa acgcacgccg gcggaggccg tgctctacgg ccgctccaag
    27721 gggctgttcc cgtcgttccc gaagaccgat cccaacacct tgacgcccgg catcaatccg
    27781 tcgcgcttcg gcgaatgggc cttcgacatc gtccatctct ttccgcattt cgtgttcaac
    27841 ccgaccgcca acagccacca gtacatgtgg ttttggccag tcgacgccga gcacgtggaa
    27901 atccgggtga tgagctatat gttcaaggcg cgcaacgccg ccgaccggct tgcccaggct
    27961 tactcgaagg tgcgcctgcg cgaggtgctg cgcgaggacc tcgccaccat ggaggccgcg
    28021 agccggatga tctcgacggg tgccttgccc tgcatggtct tctccgagca ggagatgctg
    28081 atccagaacc attacaccat cgccgacgag atgacgggcc gcaacgagcc ctccgtcgac
    28141 cggtacgcca aggcggccgc ctgagcaggg agactgaata tgacgaacca gaagcttccg
    28201 gacggcttcg ccgatctcga accctttgtg gccgaatggg cgatcgacaa cgagaaggat
    28261 cgctacctga agctgcatgc ctcacagatc gagcatctgc gcgatttcta caacatggtc
    28321 ttcgagcggc tcgaccagat catcgagcgg ctgaaccaga cgccgctctc ccagttctct
    28381 gagcaagagc ggacgctgat gaacttggcc atgactttcg ccgagacggc tcatccgatc
    28441 gacctgaaat ggcagggcgt tgatttcaac gacgcctaca cctgggagaa gttcgacttc
    28501 cgcggcgtga gcctggcgcc gatgagggag acggcttgat ggaaatggtg atcgccgcga
    28561 ggcgcatgga ggccgaggac atcgtttcgc tcgacctggt cgcgccggcg ggcggcagcc
    28621 tgccgccgtt cgaggccggg gcgcatgtcg acgtgcaggt cacgccggcg atcacacggc
    28681 aatactcgct cagcaacgac ccggccgaga cgcgacgcta tcggctggcg gtgctggccg
    28741 agccggattc gcgcggggga tcggccggcg tccatgccgg cttgccgaca ggaagcaggg
    28801 tgacgatcgg catgccgcgc aacctgttcc ggctcgatga atccgccggc cgctccatcc
    28861 tgctcgccgg cggcatcggc atcacgccgc ttttggccat ggcctatcgg ctccggacgc
    28921 tggggcggga cttcgaactg cactactgcg tgcgcggcga gtcgcgcatt gccttccggg
    28981 ccgaactggc ggccggtccg ctggcgcggc acgtttt
//
LOCUS       DASWPO010000360         6431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1961839, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000360 DASWPO010000000
VERSION     DASWPO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961839"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="VGC69_19375"
     CDS             complement(<1..1476)
                     /locus_tag="VGC69_19375"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0297496.1"
                     /translation="MTEFTASRPQSPDMPPPDALRRRLLQQGGLAALALALPGAGRAA
                     GGPGMAEAVDWGRQAIRQAMAGGRQAPAVSVALLVDGELAWQEAFGWALPGQARATPR
                     TRFNIGSVSKVLAALAAVILQDRGLIRLDTPVADYLPDFSMLSPEYRRITVRHLLSHS
                     SGLPGTNGRDIFAFAPLPGYAADTEASLARTHLKHAPGELAVYCNDGFTLIEQVVLAV
                     TCKPYPAFVQAAILDPLGMRDSGYGLAPLPAGSYGHAVWRGRTLGQEFVSAYATGGLA
                     STPGDMMRLARMFIDGGVFEGRRIVSEAGVAEMGRDQTAGLAINPCPEWRWGLGWDST
                     AQFGLAAAGVPCWEKNGGTAFYATEFFVLPQARMALLLTGSGQGYNPTAIAEGVLLRA
                     LRGRGAIRALPAPAGRDVPPQVAVEAELPREAAGIYGNNEGPYQASFAPDLSLEIHRW
                     QAGKWQPVAEGLRLRSDGWWWTDAPGASSFRFDMAPGHRYLI"
     gene            1677..2966
                     /locus_tag="VGC69_19380"
     CDS             1677..2966
                     /locus_tag="VGC69_19380"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY0297497.1"
                     /translation="MPQATPHALPSYLDAEHIGPWGIYLQQIDRVTPYLGSLARWVET
                     LKRPKRALIVDVPIEMDDGRIAHYEGYRVQHNVSRGPGKGGVRFHQDVTLSEVMALAA
                     WMSIKNAAVNLPYGGAKGGIRVDPHGLSRAELERMTRRYTSEIGVIIGPSKDIPAPDV
                     NTNAQTMAWMMDTYSMNHGSTATGVVTGKPVSLGGSLGRVEATGRGVFVVACEAARDQ
                     GVAVAGARVIVQGFGNVGGTAARLFHEAGARIVAVQDHTGTIYKPGGLDVPKLLEHVE
                     HHGGVGGYAGVQTLDPADFWSLETEFLIPAALEDQINESNAGRIRARIVVEGANGPTT
                     PAADDILHDKGIYLVPDVLANAGGVTVSYFEWVQDFSSFFWSEDEINQRLERLMREAY
                     AAVAHMAREHKVSLRTAAFIVACTRILQAREVRGLYP"
     gene            complement(2972..3817)
                     /locus_tag="VGC69_19385"
     CDS             complement(2972..3817)
                     /locus_tag="VGC69_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HEY0297498.1"
                     /translation="MRISRYAYGLLWAGLASSAGVLAGCGSTPSDNISLAGTPTPAPA
                     APTATAASPGTPLQTGDVRTVPVRSEQSRPGCRGEDCPEIKIVSVAFPDMPGLTAAVD
                     HGLAGMTWVDENLRGSYQDLSGYIAYFWRTARSGERTWLQASLRGVVGDVLSVELGTS
                     QQLAGAAHSIPATRFLLWRRSTGKEIGLDDVLVAGRHAQFVAALRLAHGQWLQAKPGY
                     REDPKGYAKLWPFVESDNVALTPQGLVVKYDAYTIAPYSDGEPEIVLPWDTVRAFVRP
                     EFLPG"
     gene            complement(3817..4617)
                     /locus_tag="VGC69_19390"
     CDS             complement(3817..4617)
                     /locus_tag="VGC69_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEY0297499.1"
                     /translation="MTEAPRNETVRTAWSDYDWQLIHEAPQAPLLHMALDAVMTDEVG
                     AGLRPPTLRIWEWAAPAVVIGRFQSVKNEVDMQAADRYGIAVTRRVSGGGAMFIEPGN
                     SITYSLSVPQALVAGMSFQESYEYLDRWVIKALHDLGIKAWYQPLNDITSEGGKIGGA
                     AQARRNGAVLHHVTMSYDIDADKMVQVLRIGREKLSDKGTASAKKRVDPLRTQTGLPR
                     EAIIERMLQTFAGMCRVSPAAIGAATLSEAQRQVREKFATPEWMTLVP"
     gene            complement(4614..4889)
                     /locus_tag="VGC69_19395"
     CDS             complement(4614..4889)
                     /locus_tag="VGC69_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HEY0297500.1"
                     /translation="MHGEYKVPGGKLVVVDLDVESGQLANVRLSGDFFLEPPEALDDI
                     NRGLTGLPSSADETELALAVASALPADVEMFGFSPEAVAVVVRRALA"
     gene            complement(4899..5603)
                     /locus_tag="VGC69_19400"
     CDS             complement(4899..5603)
                     /locus_tag="VGC69_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY0297501.1"
                     /translation="MIDLYYWTTPNGHKVTLFLEETGLPYAIHPVNIGKGEQFRPEFL
                     KIAPNNRIPAIVDQAPVGGGAPIPVFESGAILLYLAEKVGQFIPTDVRGRVEVLQWLF
                     WQMGGLGPMAGQNHHFSGYAPEPIPYAIDRYVKETNRLYGVLDQRLADRAFVAGNDYS
                     IADMACYPWIVPHERQGQKLDDFPHLQRWFDAIAARPATLRAYQKAKEVNTVPSVSDE
                     ASRRVLFGQTAQSTKR"
     gene            complement(5653..6150)
                     /locus_tag="VGC69_19405"
     CDS             complement(5653..6150)
                     /locus_tag="VGC69_19405"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY0297502.1"
                     /translation="MTSIYDFSAVDIDGATRSLGDYAGQVLLVVNVASQCGFTPQYAG
                     LDALQRGYAGLDFTVLGFPCNQFGHQEPGDEAEIKRFCETQYGVSFPMFAKIEVNGDG
                     AHPLYRWLKSQKPGLLGTEAIKWNFTKFLVGRDGQVIARFAPTDAPESLRMDIESALA
                     QAKPV"
BASE COUNT         1070 a         2240 c         2110 g         1011 t
ORIGIN      
        1 gatcaggtag cgatgcccgg gcgccatgtc gaaacggaag ctggacgccc ccggcgcgtc
       61 cgtccaccac cagccgtcgc tgcgcaacct cagcccctcg gccaccggct gccacttgcc
      121 cgcctgccag cgatggatct ccagcgacag atcgggcgcg aaactggcct ggtacgggcc
      181 ttcgttgttg ccgtagatgc ccgccgcctc gcgcggcaac tccgcttcga cggccacctg
      241 gggcggcacg tcccggccag ccggcgcggg cagggcgcgg atcgcgccgc gaccccgcag
      301 ggcgcgcagc aacacgcctt cggcgatggc cgtcgggttg tagccctgcc cgctgcccgt
      361 cagcaagagg gccatgcgcg cctggggcag cacgaagaat tccgtggcgt aaaaggccgt
      421 gccgccgttt ttctcccagc acggcacgcc cgcggccgcc aggccgaact gcgccgtgct
      481 gtcccagccc aggccccagc gccattccgg gcaagggttg atggccaggc cggccgtctg
      541 gtcgcggccc atctcggcca cgcccgcctc ggacacgatg cgccgccctt cgaacacgcc
      601 gccgtcgatg aacatccgcg ccaggcgcat catgtcgccc ggcgtgctgg ccaggccgcc
      661 ggtcgcgtag gccgatacga attcctggcc cagcgtcctg ccccgccaca cggcgtggcc
      721 gtagctgccg gcgggcaagg gggccagtcc atagccggaa tcccgcatgc ccagcgggtc
      781 gaggatggcc gcttgcacga aggccggata gggcttgcag gtgacggcca gcaccacctg
      841 ctcgatcagc gtgaaaccgt cgttgcaata caccgccagt tcgcccggcg cgtgcttgag
      901 atgcgtgcgg gccagggagg cctcggtatc ggccgcataa cccggcaggg gcgcgaaggc
      961 gaagatgtcg cgcccattgg tgccgggcag gcccgaactg tggctgagca gatgccgcac
     1021 ggtgatccgc cggtactcgg gcgagagcat ggaaaaatcc ggcaaataat cggcgacggg
     1081 cgtgtccagg cggatcagcc cacggtcctg caggatgacg gccgccagcg cggccagcac
     1141 cttgctgacc gaaccgatat tgaagcgcgt gcgcggcgtg gctctggcct ggccgggcag
     1201 ggcccagccg aaggcctcct gccaggccag ttcaccgtcc accagcaggg ccacggatac
     1261 cgccggcgcc tggcgcccgc ccgccatggc ctgcctgatg gcctggcggc cccagtccac
     1321 ggcttcggcc atgcctggac cgcccgcggc ccggcccgcg ccgggcaggg ccagcgccag
     1381 ggcggccagc ccgccttgct ggagcaggcg gcggcgtaag gcgtcgggcg ggggcatatc
     1441 aggagattgc gggcggctgg ccgtgaattc ggtcatgatt tttcctggcg taggcatgca
     1501 agagacgcgc aataggcagc cggactatag gggaagccag ctcctggcaa aacgaaaaga
     1561 tggccgatat taataagtcc ccaattaatt taaataaaca taaaataggg acttgtcttc
     1621 atttgtgtcg attgtgtggc tagaatggcg caactttcag tctggagacc acttccatgc
     1681 ctcaagcgac cccccacgcc ttgccttcct atctggatgc ggagcacatc ggcccctggg
     1741 gcatctactt gcagcagatc gaccgcgtca cgccctacct gggctccctg gcacgctggg
     1801 tcgagaccct caagcggccc aagcgggcgc tcatcgtcga tgtgcccatc gagatggacg
     1861 acggccgcat cgcccactac gagggctacc gcgtgcagca caacgtctcg cgcggcccgg
     1921 gcaagggcgg ggtgcgcttc caccaggacg tcacgctctc cgaggtcatg gccctggccg
     1981 cctggatgtc gatcaagaac gcggccgtca acctgccata cggcggtgcc aagggcggca
     2041 tccgcgtcga cccgcacggg ctgtcgcgcg ccgagctcga gcgcatgacg cgccgctaca
     2101 cctcggaaat cggcgtcatc atcggcccct ccaaggatat tccggcgccc gacgtcaaca
     2161 ccaacgccca gaccatggcc tggatgatgg acacctattc catgaaccac ggctccaccg
     2221 ccaccggcgt ggtcaccggc aagcccgtct ccctgggcgg cagcctgggc cgcgtcgagg
     2281 ccaccggccg aggcgtcttc gtcgtggcct gcgaggccgc gcgcgaccag ggcgtggccg
     2341 tcgccggcgc gcgcgtcatc gtgcagggct tcggcaacgt cggcggcacc gccgcgcgcc
     2401 tgttccacga agccggcgcc aggatcgtcg ccgtgcagga ccacaccggc acgatctaca
     2461 agcccggcgg cctggacgtc cccaagctgc tcgaacacgt cgagcaccat ggcggggtgg
     2521 gcggctacgc cggcgtccag acgctggacc cggccgattt ctggagcctg gagaccgaat
     2581 tcctcattcc cgccgccctc gaggaccaga tcaacgaaag caacgccggc cggatccgcg
     2641 ccaggatcgt cgtcgagggc gccaacggcc ccaccacgcc ggcggccgac gacatcctgc
     2701 acgacaaggg catctacctg gtgcccgacg tgctggccaa cgcgggcggc gtcacggtga
     2761 gctatttcga atgggtgcag gacttctcca gctttttctg gagcgaggac gagatcaacc
     2821 agcgcctcga gcgcctcatg cgcgaagcct atgcggccgt cgcccacatg gcccgcgagc
     2881 acaaggtcag cctgcgcacc gccgccttca tcgtggcgtg cacgcgcatc ctgcaggcgc
     2941 gcgaagtgcg cgggctgtat ccgtaatcga atcagcccgg caggaactcc ggccgcacga
     3001 acgcgcggac cgtatcccag ggcagcacga tctcgggttc tccgtcggag tacggcgcga
     3061 tcgtgtacgc gtcgtacttc accaccagcc cttgcggggt gagcgccaca ttgtcgcttt
     3121 ccacgaaagg ccacagcttg gcatagccct tcgggtcctc ccgatagcct ggcttggcct
     3181 gcagccattg cccgtgcgcc aggcgcagcg ccgccacgaa ctgcgcgtgc cggcccgcga
     3241 ccagcacgtc gtcgagcccg atctccttgc cggtgctgcg gcgccatagc aggaagcgcg
     3301 tagccggaat cgaatgcgcc gcgcccgcga gctgttggct cgtgcccagc tcgacgctga
     3361 gcacgtcgcc caccacgccc ctcaggctgg cttgcagcca agtccgctcg ccggagcgcg
     3421 ccgtgcgcca gaaatacgcg atatagcccg acaagtcctg gtaggaaccg cgcaggttct
     3481 cgtccaccca cgtcatgccc gccaggccgt gatcgactgc ggccgtcagt cccggcatgt
     3541 ccggaaacgc cacgctgact atcttgatct ccgggcagtc ttcacccctg caacccggcc
     3601 gggattgttc ggaacgtacc ggcacggtgc gcacgtcgcc ggtctggagc ggcgtgcctg
     3661 gcgacgcggc cgttgccgta ggcgccgccg gggcgggcgt aggcgtgccc gccaggctga
     3721 tattgtccga aggggtgctg ccgcatccgg ccagcacgcc ggccgacgag gccaagccgg
     3781 cccagagcag gccgtacgca tatctgctga tccgcatcat ggcaccaggg tcatccattc
     3841 aggggtggcg aacttctcgc gcacctggcg ctgcgcctcg ctcagcgtcg ctgcgccgat
     3901 ggcggccggc gacacccggc acatgcccgc gaaagtctgc agcatgcgct cgatgatggc
     3961 ctcgcgcggc aaacccgtct gggtgcgcag cgggtcgacc cgcttcttgg cgctggccgt
     4021 gcccttgtcg gacagttttt cgcggccgat gcgcagcacc tgcaccattt tgtcggcgtc
     4081 gatgtcgtac gacatggtca cgtggtgcag caccgcgccg ttgcgccgcg cctgcgccgc
     4141 cccgccgatc ttgccgccct cggaagtaat gtcgttgagc ggctggtacc aggccttgat
     4201 gcccaggtcg tgcagcgcct tgatcaccca gcggtccagg tactcgtagg actcctggaa
     4261 gctcatgccc gccaccaacg cctgcggcac cgacagcgag taggtaatgc tgttgcccgg
     4321 ctcgatgaac atggccccgc cgccgctcac ccggcgcgtc acggcgatgc cgtagcggtc
     4381 ggcggcctgc atgtccactt cgttcttcac cgactggaag cggccgatca ccacggctgg
     4441 cgcggcccat tcccagatgc gcagggtagg aggccgcagg cccgcgccga cctcgtcggt
     4501 catgacggcg tccagcgcca tgtgcagcag gggcgcctgc ggcgcctcgt ggatgagctg
     4561 ccagtcgtag tcgctccagg ccgtgcggac ggtctcgttc ctgggggcct cggtcatgcc
     4621 agcgccctcc gcaccaccac ggccaccgct tcgggcgaga agccgaacat ttcgacgtcg
     4681 gcgggcaatg cgctggccac ggccagcgcc agctcggtct cgtccgccga ggacggcagg
     4741 cccgtcaggc cgcggttgat gtcgtccagc gcctccggcg gctccaggaa gaaatcgccg
     4801 gagaggcgca cgttggccag ttggccgctt tccacgtcca ggtcgaccac caccagcttg
     4861 ccgcctggca ccttgtattc accatgcatg gccagccctc agcgcttggt cgactgggcc
     4921 gtctggccga acaggacgcg gcgcgaggcc tcgtcgctca ccgatggcac ggtgttgact
     4981 tccttggcct tctggtaggc gcgcagcgtg gccggacgcg ccgcgatggc atcgaaccag
     5041 cgctgcagat gggggaaatc atcgagcttc tggccctggc gctcgtgcgg gacgatccag
     5101 gggtagcagg ccatgtcggc gatggaatag tcattgcccg ccacgaaggc ccgatccgcc
     5161 agccgctggt cgagcacgcc atacaagcgg ttggtttcct tgacgtagcg atcgatggcg
     5221 tagggaatgg gctcgggcgc atagcccgag aaatggtgat tctgcccggc catggggccc
     5281 aggccgccca tctgccagaa gagccactgc agcacctcca cgcggccgcg cacatccgtc
     5341 gggatgaact ggccgacctt ctcggccaag tacagcagga tggcgcccga ctcgaagacc
     5401 gggatcggcg cgccgccgcc gaccggcgcc tggtcgacga tggccgggat gcggttgttg
     5461 ggagcgatct tcaggaactc gggccggaac tgctcgccct tgccgatatt gaccgggtgg
     5521 atggcgtagg gcaggccggt ttcttcaagg aagagcgtga ctttgtggcc gttgggcgtg
     5581 gtccagtaat acaggtcgat cattatgtgc tgtccggatt gtgtacttaa gcatcgactc
     5641 aatgcgatac catcataccg gcttggcctg cgcgagcgcc gactcgatgt ccatgcgcag
     5701 ggactccggc gcatcggtgg gggcaaagcg cgcgatcacc tggccgtccc ggcccaccag
     5761 gaacttggtg aaattccatt tgatggcctc ggtgcccagc aggccgggct tctgcgactt
     5821 gagccaacgg tacagcggat gggcgccgtc gccgttgacc tcgatcttgg cgaacatggg
     5881 aaaactcacg ccgtactggg tttcgcagaa gcgcttgatc tcggcctcgt cgcccggctc
     5941 ctggtggccg aactggttgc agggaaaccc cagcaccgtg aaatcgaggc cggcatagcc
     6001 gcgctgcagc gcatcgagcc ccgcatactg cggcgtgaag ccgcattgcg aggctacgtt
     6061 gaccaccagc agcacctgcc cggcatagtc tcccaggctg cgcgtggcgc cgtcgatgtc
     6121 gacggcggaa aaatcgtaaa tagaggtcat gcgtctggct cccggccggc gtgcggccat
     6181 gcgtatatgc ctttataccg ccgacccaaa caaaacgggc cgtcatgtcc cgcatgacgg
     6241 cccgtttgga gagaagcgcc gggctcagtt cagcctggcg ccgcccacca cgaagcggcc
     6301 cagccccgac accagcaagg acaaggcgcc gaacaggtac atggcctgca gttccagcgc
     6361 atagccgccg gtgttcgggt cgaggctgaa gaactggctg gtatgcacca gcagcagggc
     6421 cacgatcatg t
//
LOCUS       DASWPO010000361         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_1337482, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000361 DASWPO010000000
VERSION     DASWPO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337482"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VGC69_19410"
     CDS             <1..279
                     /locus_tag="VGC69_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0297503.1"
                     /translation="LYDSSADMLRDANAGRVNAIVIDYPIAAYGIAQGTFPKLHMVKS
                     FKPTLPGEIGIATRKGDAELLGKISKGLANIKANGKLDAIIKKWGLAD"
     gene            324..977
                     /locus_tag="VGC69_19415"
     CDS             324..977
                     /locus_tag="VGC69_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY0297504.1"
                     /translation="MSQFFIDAQEYLPILMQGVKLTIIVTLGTLVLSTLLGLVWAVMR
                     ASGIRALAWASACVVNVLRGIPVIVVLFYIYFVMPDVGISLTALQAAIIGLGVAYSAY
                     HAENFRAAIQAIDPGQIEAAMSMGMGWGLTMRRVILPQAFKIVLPPYGNIMIMMLKDS
                     SQASTITVAELALQGKLIAVSTFKNATVFNLVALLYLIMCVPLILIVRHFEKRGARR"
     gene            986..1717
                     /locus_tag="VGC69_19420"
     CDS             986..1717
                     /locus_tag="VGC69_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY0297505.1"
                     /translation="MIQLHGVHKRFGDLEVLKGIDAKVDRGEVVCVIGPSGSGKSTLL
                     RCVNGLERYNSGEIVVDGIKVDCDAPSIVDIRTQVAMVFQRFNLFPHRTALENVIEGP
                     VYVKKEPRAQVLERGRALLDKVGLADKVDAYPSQLSGGQQQRVAIARALAMQPKAILF
                     DEPTSALDPELVGDVLGVMRQLAEAGLTMLVVTHEMSFAREVADRVLFCDGGVVVEQG
                     AAREVLNHPQHARTQDFLHRVLHPM"
     gene            1727..3019
                     /locus_tag="VGC69_19425"
     CDS             1727..3019
                     /locus_tag="VGC69_19425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0297506.1"
                     /translation="MAQASPFPLSPALWAATAVAAPQTASLSESVKADVLIIGAGYAG
                     LSAALHLAEQGVNAVVLEAREIGFGGSGRNGGQVIPGLKYDPDDLMAMYGPERGARLV
                     QFVGGTADTVFGLIDKHGMDVPRLRVGWIQGAHTAKALQLTESRARQWQRLGANVEVL
                     DRAGAQALMGNDRYLGGWVDKRAGSIQPLSYVRGLAKAALAAGARIYTDSPVAGLRRE
                     GSQWTARTAGGAQVAAERVVMCTNAYGGKLWPGLQSSIIDACTYQVATRPLPPEIRST
                     ILPQGHFVSDTRNLLLYFRLDHQGRLLMGGRGPFREPKGPADWAHLERVLAKMFPQAA
                     GQPIEYRWCGRVAITRDYLPHLHEPAPGLLADIGCQGRGVGLQTAMGRAMAQYLVSGD
                     QKALPVPVTPITGFPLYGLRRLYVGAVVNYYRMIDGGL"
     gene            complement(3054..4400)
                     /locus_tag="VGC69_19430"
     CDS             complement(3054..4400)
                     /locus_tag="VGC69_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0297507.1"
                     /translation="MAEAEWLAHYVATDGAGNLVNSIGDLVRSGRLEAGARLPTVRAL
                     GARMGVSPATVAAAWSQLRRQGLIETRRRGGTVVVYDASAPDASWQGLGTGQSLDLAQ
                     GLVDRALLPELGTAFQAALGNRHVHGAAKDHCIAPLQEAAAPTWPFRPQAWSTAGSGT
                     EGALLAVAAAVRRAGQPRVAIESPTSPRLLDLCRTLGLSLLPVDCDDAGPLPESLREA
                     LARDPAAFIYQPRAQIPLGHSVPQERVRALADELARAPRTVAVEDDFFGPLARDGVHS
                     IGAYLPERVLFVRTYCNAYGIDLRTCILAGAALLVDTVRELRSHGAAMNSRILQGALA
                     HLLTDPDTEAWIEAARERYASRRVALVEALRGEGFAVRNRDGLGIWLPVEKEEQAIVN
                     LAEHGISAGGGSRCFPAGPAGSHLRLSTGRLPDNPGHIVEIAQAFAAAAGHPRPAR"
     gene            4700..5518
                     /locus_tag="VGC69_19435"
     CDS             4700..5518
                     /locus_tag="VGC69_19435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0297508.1"
                     /translation="MSSPSASAAPPRRRRWPDRPGLARVALPLGVLAAVAVLQEGLTR
                     SGVVNPVRFPPMSSVVSTLFDEAASGRLWAPLAGTGANWLASLALSFLVALALAVALN
                     ALPLVHALMAPVIAFLRPMPCTALIPLVILSLGATAQGMVFLTTFGTVWQMLPVLARA
                     VGRVDDVARDTARVFGFTPLQVLRWLTLRALEPHILTALRIGATASLTLLISMELLTG
                     ASGVGRIISLAYAGSNLKLMYAYVLLSGLLGIVVNLASRQVLAHRLRYLEGGAR"
     gene            5515..>6210
                     /locus_tag="VGC69_19440"
     CDS             5515..>6210
                     /locus_tag="VGC69_19440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0297509.1"
                     /translation="MSRRILRSFILRPFASRRFALGTLGAVLAIGAWLGLSWHSGNAF
                     FPPLHRMLLDAASYWFTAAGLADIASTLTNMGLGLVLGIALGAAGGLLIGQVRVLDLA
                     LSPLIEFVRAIPSVALLPAAIGILGIGSAMKVASIALSVMWPVLLSTIDGMRHLPAQW
                     LDTARVFHFSAWRRQLLVVVPALVPRILAGVNVAVPLSLVVAVTSEMIGTTAGIGSVV
                     LNAQYTFDVERMWS"
BASE COUNT          895 a         2122 c         2114 g         1079 t
ORIGIN      
        1 ctgtatgact ccagcgccga tatgctgcgc gatgccaacg cgggccgtgt caacgccatc
       61 gtgatcgact atccgatcgc tgcctacggc attgcgcagg gcacgttccc caagctgcac
      121 atggtcaaga gcttcaagcc cacattgccg ggtgagatcg gtatcgccac ccgcaagggc
      181 gatgccgagt tgctgggcaa gatcagcaag ggcctggcca atatcaaggc caacggcaag
      241 ctcgacgcca tcatcaagaa atggggcttg gccgactgac gcgggcaagc tgaacgctgc
      301 aagccttcat ggaatcctag gctatgtctc aattcttcat tgacgcgcag gagtatctgc
      361 cgatcctgat gcagggcgtc aagctcacca tcatcgtgac cttgggcacg ctggtgctgt
      421 cgaccctgct gggcctggtg tgggccgtca tgcgcgcatc gggcattcgc gcgctggcct
      481 gggccagtgc ctgcgtggtc aacgtgctgc gcggcattcc ggtcatcgtg gtgctgttct
      541 acatctactt cgtgatgccg gacgtcggca tttcgctgac ggccctgcag gcggccatca
      601 tcggcctggg cgttgcgtat tcggcctacc atgccgagaa cttccgcgcg gccatccagg
      661 ccatcgaccc tgggcagatc gaggccgcca tgtcgatggg catgggatgg gggctgacca
      721 tgcgccgggt gatcctgccc caggccttca agatcgtgct gccaccctac ggcaatatca
      781 tgatcatgat gctcaaggat tcgtcccagg cctccaccat caccgtggcc gaacttgccc
      841 tgcagggcaa gctgatcgcg gtgtccacct tcaagaacgc cacggtattc aacctggtgg
      901 ccttgctcta cctgatcatg tgcgtgccgc tcatcctcat cgtgcgccat ttcgagaagc
      961 ggggggcgcg tcgatgagcg aacccatgat ccagctgcat ggcgtgcaca agcgtttcgg
     1021 cgatctggag gtgctcaagg gcatcgacgc caaggtcgat cgtggcgaag tggtctgcgt
     1081 catcgggccg tcgggctcgg gcaagtccac gctcctgcgc tgcgtcaacg gcctggagcg
     1141 ctacaacagc ggcgagatcg tcgtcgacgg catcaaggtc gactgcgacg cgccgtccat
     1201 cgtggacatc cgcacccagg tggcgatggt gttccagcgc ttcaacttgt tcccgcaccg
     1261 cacggccctg gagaacgtca tcgaggggcc ggtctacgtc aagaaggaac cgcgcgccca
     1321 ggtgctcgag cgcggccgcg ccctgctcga caaggtgggc ctggccgaca aggtcgacgc
     1381 ctatccctcg cagctctcgg gcgggcagca gcagcgcgtg gccattgccc gggcgctggc
     1441 catgcagccc aaggccatcc tgttcgacga gcccacctcg gcgctcgacc ccgagctggt
     1501 gggcgacgtg ctgggcgtca tgcgccagct ggccgaggcc ggcctgacca tgctggtcgt
     1561 cacccatgaa atgagcttcg cccgcgaggt ggccgaccgc gtgctgttct gcgacggcgg
     1621 cgtggtcgtc gagcaggggg ccgcccgcga agtgctgaac catccccagc atgcccgcac
     1681 gcaggacttc ctgcaccgcg tgctgcatcc gatgtaagga atccccatgg cgcaagcctc
     1741 accctttccc ttgtcgccgg cgttgtgggc ggccacggcc gttgccgcgc cgcagacggc
     1801 gtccctgtcc gagtccgtca aggcggacgt gctcatcatc ggcgcgggct atgccggcct
     1861 gagcgccgcg ctgcacctgg ccgagcaggg cgtcaacgcc gtggtgctcg aggcgcgcga
     1921 gatcggtttc ggcgggtcgg gccgcaatgg cgggcaggtc attcccggcc tcaagtacga
     1981 tccggacgat ctgatggcca tgtacggccc cgagcgtggc gcgcggctgg tgcagttcgt
     2041 cggcggcacg gccgacacgg tcttcgggct catcgacaag cacggcatgg acgtgccgcg
     2101 cctgcgcgtg ggctggatac agggggcgca tacggccaag gccctgcagc tgaccgagtc
     2161 gcgggcccgg cagtggcagc gcctgggcgc caatgtcgag gtgctcgacc gcgcgggcgc
     2221 ccaggccctg atgggcaacg accgctatct gggcggctgg gtcgacaagc gggccggaag
     2281 cattcagccc ctttcttatg tgcggggctt ggccaaggcg gcgctcgccg cgggcgcgcg
     2341 catttacacc gacagcccgg tggcgggatt gcgccgcgag ggttcgcaat ggacggcccg
     2401 caccgccggc ggcgcgcagg tcgcggcaga acgcgtggtg atgtgcacca atgcctacgg
     2461 cggcaagctc tggccggggc tgcagtcgtc catcatcgat gcctgcacct atcaggtggc
     2521 cacccggccg ctgccccccg agatccgcag caccatcctg ccgcaagggc atttcgtgtc
     2581 cgacacgcgc aatctgctgc tgtacttccg gctcgaccac cagggccgcc tgctgatggg
     2641 gggccggggt ccgttccgcg agcccaaggg ccccgccgat tgggcccacc tggaacgcgt
     2701 gctggccaag atgttcccgc aggccgcggg ccagcccatc gaataccgct ggtgcggccg
     2761 cgtggccatc actcgcgact acctgccgca cttgcatgaa ccggcgcccg gcctgctggc
     2821 cgacatcggc tgccaggggc gcggcgtggg cctgcagacc gcgatgggcc gcgccatggc
     2881 gcaatacctg gtgagcggcg accagaaggc cttgcccgtg ccggtcacgc cgatcacggg
     2941 ctttccgctg tacggcttgc gccgcttgta cgtcggcgcc gtcgtcaact actaccgcat
     3001 gatcgacggc gggctgtagc ggcgggcgca ggcgaagtgg gaggccatgc gcgtcagcgc
     3061 gccggccggg gatgccccgc cgcggcggca aaggcctgcg cgatctcgac gatgtggccg
     3121 ggattgtccg gcaggcgccc cgtggacagg cgcaggtgcg agcccgcggg cccggccgga
     3181 aagcagcggc tgccgccgcc tgccgaaatg ccatgctcgg ccaggttgac gatggcctgt
     3241 tcttcttttt ccaccggcag ccagatgccc aggccgtcgc ggttgcgcac ggcaaacccc
     3301 tcgccgcgca gggcttccac cagcgccacg cgccgcgagg catagcgttc gcgggcggcc
     3361 tcgatccatg cctcggtgtc cgggtccgtc agcagatggg cgagcgcgcc ttgcaggatg
     3421 cggctgttca tcgcggcgcc gtggctgcgc agttcccgga cggtgtcgac gagcaaggcc
     3481 gcgccggcca ggatgcaggt ccgcaggtcg atgccgtagg cattgcaata agtgcgcacg
     3541 aacaggacgc gctcgggcag gtaggcgccg atgctgtgga cgccgtcgcg cgcgagcggg
     3601 ccgaagaaat cgtcttccac ggccacggtg cgcggcgcgc gcgcgagctc gtcggccagg
     3661 gcgcgcacgc gctcctgggg cacggagtgg ccaaggggaa tctgggcgcg gggctggtag
     3721 atgaatgccg ccggatcccg cgccagcgct tcgcgcagcg attcgggcag cgggcccgcg
     3781 tcgtcgcagt ccaccggtag cagcgacagc ccgagcgtgc ggcagaggtc gagcagcctg
     3841 gggctggtgg gcgattcgat ggccacccgc ggctggccgg cccggcgcac ggcggcggcc
     3901 acggcaagca gggcgccttc ggtgccgctg cccgccgtgc tccatgcctg gggcctgaac
     3961 ggccaggtgg gggcggcggc ttcctgcagg ggcgcgatgc aatgatcctt ggctgcgccg
     4021 tggacgtggc ggtttcccag ggcggcctgg aacgccgtcc cgagttcggg caacagggcg
     4081 cgatcgacca ggccctgggc gagatccagc gattgcccgg tgcccaggcc ttgccaggaa
     4141 gcatccggcg ccgaggcatc gtagacgacc acggtgccgc cccggcggcg ggtttcgatc
     4201 aggccttgcc ggcgcaattg cgaccaggcc gcggcgaccg tggccgggct gacgcccatg
     4261 cgggcgccca gcgcgcgcac ggtcggcagc cgcgctccgg cttccaggcg tccggagcgg
     4321 accaggtccc cgatgctatt gaccaggttg ccggcgccat cggtcgcgac gtagtgggcc
     4381 agccactccg cctccgccaa ttgtcccgat tcgtctgaga cctcgcgccg gttcaatccc
     4441 cttcctcccc aggccgtccg cccggatccc ggcatggcgc ctgattgtga acaaattcca
     4501 tatttttcga atcatacctg ccgcgaagca ggatgaaaca cagtggatag ccccgaattt
     4561 tccagcctta tcaggcgatc cgttagggct tatttgatct gaacatattt aataaaaata
     4621 ataaacatat tttcattgga tattggcgcc ccatgcggtc cggaggattc aatacggcat
     4681 ggaatcgctg atcccgttca tgtcttcccc atccgccagc gccgcgccgc cgcgccggcg
     4741 ccgctggccc gatcggcccg gccttgccag ggtcgcgctg ccgctgggcg tgctggccgc
     4801 cgtcgccgtc ctccaggaag gcctgacgcg ttcgggcgtg gtcaatccgg tgcgcttccc
     4861 gccgatgagc agcgtcgtgt cgacgctgtt cgacgaggcg gccagcggcc gcctatgggc
     4921 gccgctggcc ggcacgggcg cgaactggct ggcatcgctg gcgctgtcct tcctcgtcgc
     4981 cctggcgctg gcggtggcgc tcaatgcctt gccgctcgtc cacgcgctga tggcgccggt
     5041 catcgctttc ctgcgcccca tgccctgcac ggcgctgatc cccctggtca ttctttcctt
     5101 gggggccacg gcgcaaggca tggtcttcct gacgaccttc ggcacggtct ggcaaatgct
     5161 gccggtgctg gcgcgcgcgg tcggccgcgt cgacgacgtc gcccgcgata cggcgcgggt
     5221 gttcgggttc acgccgctgc aggtcctgcg ctggctcacg ctgcgcgcgc tggagcccca
     5281 catcctgacc gccctgcgca tcggcgccac cgcctcgctg acgctgctga tcagcatgga
     5341 attgctgacc ggagcttcgg gtgtcggccg gatcatctcc ctggcgtatg cgggttcgaa
     5401 cctgaagctg atgtatgcct atgtcctgct ctcggggctg ctcggcatcg tggtcaacct
     5461 ggcgtcgcgc caggtcctgg cgcaccgcct gcggtatctg gaaggcggtg cgcgatgagc
     5521 cggcgcattc ttcgttcctt catcctccgc cccttcgctt cccgccggtt tgcgctgggc
     5581 accctgggcg cggtcctggc gatcggcgcc tggctcgggc tcagctggca ttccggaaat
     5641 gccttcttcc cgccgctgca tcgcatgctg ctggacgccg cctcgtactg gttcacggcc
     5701 gccggcctgg cggacatcgc ttccacgctg accaatatgg ggctaggcct ggtgctcggc
     5761 atcgccctgg gcgccgccgg cgggctgctc atcggccagg tgcgcgtgct cgacctggcc
     5821 ttgtctcccc tgatcgagtt cgtgcgcgcg attcccagcg tggcgctgct gcccgcggcc
     5881 attggcattc tcgggatagg cagcgccatg aaggtcgcca gcatcgcctt gagcgtcatg
     5941 tggcccgtgc tcctgagcac gatcgacggc atgcggcact tgcccgccca atggctcgat
     6001 acggcgcgcg tctttcattt cagcgcctgg cgccggcagt tgctggtcgt cgtgccggcg
     6061 ctggtgccgc gcatcctggc cggcgtcaac gtcgccgtcc cgctgagcct ggtggtggcc
     6121 gtcaccagcg aaatgatcgg caccacggcg ggcatcggtt ccgtggtgct caacgcgcaa
     6181 tacaccttcg atgtcgagcg catgtggtcg
//
LOCUS       DASWPO010000362         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2052415, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000362 DASWPO010000000
VERSION     DASWPO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052415"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1635)
                     /gene="lysS"
                     /locus_tag="VGC69_19445"
     CDS             complement(103..1635)
                     /gene="lysS"
                     /locus_tag="VGC69_19445"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY0297510.1"
                     /translation="MTEQSTAPQPQDENRLIAERRAKLAKLREAGVAFPNDFVPDAHA
                     AHLHAKYDDQEQEALQAAHVQVKVAGRMMLKRVMGRASFATLQDGSGRIQIYLDRGTL
                     GEESYAAFKQWDIGDLIAIEGSVFKTNKGELSVHAGTARLLAKSLRPLPDKFHGVADQ
                     ELRYRQRYVDLIMTEETRRTFAARSKAVGAIRQFMLDAGFLEVETPMLHPIPGGAAAK
                     PFITHHNALDMQMFLRIAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMME
                     FYAAYADYRWQMDFTESLIRQAAIAAAGSAVLQYQGRELDLARPFHRLTICEAILGHA
                     PEKGHHYTQQQLDDADFVRAELKRLGADVNGPVLSRAGLGALQLALFEETAESQLWEP
                     TYIIDYPVEVSPLARESDTRPGITERFELFITGREIANGFSELNDPEDQAARFRAQVE
                     AKDAGDEEAMYYDADYIRALEYGMPPTGGCGVGIDRLVMLLTDSPSIRDVILFPHLRR
                     ED"
     gene            complement(1645..2400)
                     /locus_tag="VGC69_19450"
     CDS             complement(1645..2400)
                     /locus_tag="VGC69_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0297511.1"
                     /translation="MIDTIAVLTGASRGLGASLARGLLRPGVKLVTLARRDDAELAAQ
                     AKAAGAELEQIQVDLSDLAAAEAAGQRIAALLAPGARRYLLINNAGTVDPVVASPAMT
                     DGPAIARAFNLNVTAVMLLTARFLQAVAGLDAQRRVINISSGAGRSPTAGWGVYCATK
                     AALDIYTRVLNQEQGAAGVRAVSLAPGIVDTDMQAAIRSSDPAHFPALAKFQDFKKSG
                     ALAQPDAVAARILAYLDRDDFGATEIDDIRNYQ"
     gene            complement(2447..>2566)
                     /gene="prfB"
                     /locus_tag="VGC69_19455"
     CDS             complement(2447..>2566)
                     /gene="prfB"
                     /locus_tag="VGC69_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY0297512.1"
                     /translation="VLDQSRIKDLRTNVEISNTQKVLDGDLDPFIQESLKQGI"
BASE COUNT          353 a          769 c          994 g          450 t
ORIGIN      
        1 cgttcttccg cgagcaggtc gtggagtacg ccaagctgat caagcgctac cagatacccc
       61 agcagtaggc cggagggggg ccggacccgg tccggcccgg gctcagtctt cccggcgcag
      121 gtgggggaaa aggatcacgt cgcggatgct ggggctgtcg gtgagcagca tgaccaggcg
      181 gtcgatgccc acgccgcagc cgccggtggg cggcatgccg tattccagtg cgcggatgta
      241 gtcggcgtcg tagtacatgg cctcttcgtc gccggcgtcc ttggcctcga cctgggcgcg
      301 aaagcgcgcg gcctggtcct cgggatcgtt gagctcggag aagccgttgg cgatctcgcg
      361 gccggtgatg aagagctcga agcgctcggt gatgccgggg cgcgtgtcgg attcgcgggc
      421 cagcggcgag acttccaccg gatagtcgat gatgtaggtg ggttcccaca gctgcgactc
      481 ggcggtctct tcgaagagcg ccagctgcag cgcgcccagg ccggcgcgcg acagcacggg
      541 gccgttcacg tcggcgccca gccgcttgag ttcggcgcgc acgaagtcgg cgtcgtcgag
      601 ctgttgctgg gtatagtggt ggcccttttc cggagcgtgc ccgaggatgg cctcgcagat
      661 ggtcaggcgg tggaagggcc tggccaggtc cagctcgcgg ccctggtact gcagcacggc
      721 gctgccggcg gcggcaatcg ccgcctggcg gatcagcgat tcggtgaagt ccatctgcca
      781 gcggtagtcg gcataggccg cgtagaactc catcatggtg aattcggggt tgtgccgcgg
      841 gctcacgcct tcgttgcgga agttgcggtt gatctcgaac acgcgctcga agccgcccac
      901 caccaggcgc ttgaggtaga gctcgggcgc gatgcgcagg aacatctgca tgtccagcgc
      961 attgtggtgg gtgatgaagg gcttggccgc cgcgccgccg gggatggggt gcagcatggg
     1021 cgtctcgact tccaggaagc cggcgtccag catgaactgg cggatggcgc ccacggcctt
     1081 gctgcgcgcc gcgaaggtgc ggcgcgtttc ttcggtcatg atgaggtcga cgtagcgctg
     1141 gcggtagcgc agttcctggt cggccacgcc gtggaacttg tcgggcaggg gccgcaggga
     1201 cttggccagc aggcgcgccg tgccggcgtg caccgagagc tcgcccttgt tggtcttgaa
     1261 gaccgaacct tcgatcgcga tcaggtcgcc gatgtcccac tgcttgaagg ccgcgtagga
     1321 ctcctcgccc agggtgccgc ggtccaggta gatctggatg cggccgctgc cgtcctgcag
     1381 ggtggcgaag ctggcgcggc ccatgacgcg cttgagcatc atgcggccgg ccaccttgac
     1441 ctgcacgtgg gcggcctgca gggcttcctg ctcctggtcg tcgtacttgg cgtggaggtg
     1501 ggcggcgtgc gcgtcgggca cgaagtcgtt ggggaaggcg acaccggctt cccgcagctt
     1561 ggccagcttg gctcgccgct cggcgatcaa gcggttttcg tcttgcggct gcggagcggt
     1621 cgattgttcg gtcatggcgg ggaattattg gtaattgcgg atgtcgtcga tttcggtggc
     1681 gccgaagtcg tcgcggtcca ggtaggcgag gatgcgggcg gccacggcgt ccggctgggc
     1741 cagggcgccg ctcttcttga agtcctggaa cttggccagg gccgggaagt gggccgggtc
     1801 gctggaacgg atggcggcct gcatgtcggt gtcgacgatg ccgggggcca gcgacacggc
     1861 gcgcacgccg gccgcgccct gttcctggtt gagcacgcgg gtatagatat cgagcgcggc
     1921 cttggtggcg cagtacacgc cccagcccgc ggtggggctg cggccggcgc ccgacgagat
     1981 gttgatcacg cggcgctgcg cgtcgaggcc ggcgacggcc tgcaggaagc gcgcggtcag
     2041 cagcatgacg gccgtgacgt tgaggttgaa ggcccgcgcg atggcggggc cgtcggtcat
     2101 ggccgggctg gccaccaccg ggtccacggt gccggcgttg ttgatgagca ggtagcggcg
     2161 cgcgccgggc gcgagcaggg cggcgatgcg ctggccggcg gcctcggcgg cggcgaggtc
     2221 ggacaggtcg acctggatct gctccagctc ggcgccggcg gccttggcct gggcggccag
     2281 ttcggcgtcg tcgcggcgag ccagcgtgac cagtttcacg ccggggcgca gcaggccgcg
     2341 cgccagggag gcgcccaggc cgcgcgaggc gccggtaaga acggcaatgg tatcgatcat
     2401 gtcttggttt ccccagtatg gatgtcggag cgaggcgcga ggcgggtcag atgccttgct
     2461 tgaggctttc ctggatgaag ggatccaggt cgccgtcgag cactttctgc gtgttggaga
     2521 tttcgacgtt ggtgcgcagg tccttgatgc ggctctggtc gagcac
//
LOCUS       DASWPO010000363        19401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2498478, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000363 DASWPO010000000
VERSION     DASWPO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 19401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19401
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498478"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGC69_19460"
     CDS             <1..259
                     /locus_tag="VGC69_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY0297513.1"
                     /translation="PTFKLTFKPDGTFEPARLEVPAGRFKIDLVNASKEPVEFESIPL
                     RKEKVLGPGVESFVVITISRPGEYPFFDDFHPNVKGTLVVK"
     gene            277..1134
                     /locus_tag="VGC69_19465"
     CDS             277..1134
                     /locus_tag="VGC69_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEY0297514.1"
                     /translation="MEQVLFIVWRECVEALLVVGILYSWLKASPEGRRGLPYLWGGVA
                     GGLALALALALVLLGVSEWLSDTGQEWFQGIMSLVACALVVQMVVWMKQHGRTLKREL
                     ETGARDSIARDNWWGVFTLVAIAVAREGSETVVFLYGTISAAGSGAAGGQSFAALTLA
                     GVAGLAAAGLTFWLLQLGGKLITWRRFFKLTEILLLLLAGSLLVGGLDHLISLDVMPT
                     LMDPVWDSSWLLSDSAGLGKILADFAGYRAYPALSSLLCWLAYWVVVWALLRRVGRQS
                     GAPARQVAQ"
     gene            1177..2577
                     /locus_tag="VGC69_19470"
     CDS             1177..2577
                     /locus_tag="VGC69_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY0297515.1"
                     /translation="MTAVIGRASVRFADFLRDHAPLLRKLQWGIVAVYAFLLAVPAAM
                     PLPGNTASVFNNLTIAAQFAFWGIWWPFVLVSMPVLGRAWCGWLCPEGMLTEWASEHG
                     RGHAIPKWMRWGGWPFVAFALTTVYGQLISVYQYPLAVLVVLGGSTAGAMAVGWYYGH
                     SKRVWCKYLCPVNGVFSLLAKLAPWHYKVSEDAWRQSLSTERRRVIRIESINCAPLVP
                     LRHMKGASDCHMCGRCSGYRDAIALTPRSPEAEIVEVAQGDAWQTALICLGLMGIAVG
                     AFLWSASPWYVDLKQLIATWLIDHNVMWPLADNAPWFILTHYPEVNDSFSWLDGALIL
                     VFAMGAAAVVGGSLYLALWIADRLAPMQGAAGRFAGLHKLAQALIPSAGVGVFLGLSA
                     TTITLLQHEGMATGWVRPVRFTLLALALAWSLRLAWRLLGRRTAGAGRRALAWLAFAA
                     GLAPFAAAWVLFFAVW"
     gene            2657..3526
                     /locus_tag="VGC69_19475"
     CDS             2657..3526
                     /locus_tag="VGC69_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0297516.1"
                     /translation="MPSYTFHLVNVFAESTFGGNPLCVFDDARGMDEAAMQSLALQFN
                     LSETTFVLPSGEADAQVRIFTPGYEMRFAGHPTLGTAHVVRGVRGLGNDLTLQFKAGS
                     VPVRAQGDLWTLVPPLDGAPRTAPPTQSPAEIAALLGLSPDDLLETPLWVDTGADQLL
                     VPVKTADAVRRAQPDAARLAQWPASSLGHKTAYVFAFTGDRQDGRDVVLSRYFFSKEG
                     GGVAEDPGTGSACANLGGWLLARGRALPSSILVRQGEQAGRPCTLYLDVDQDGTIRVG
                     GRVIALGKGEIAL"
     gene            complement(3521..5395)
                     /locus_tag="VGC69_19480"
     CDS             complement(3521..5395)
                     /locus_tag="VGC69_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY0297517.1"
                     /translation="MADSHAAGLPKSRPVGHRPWTAVADGLLGRLVEIPAAILVAAEV
                     VILFTGIVARYVFRNPLVWSDELASILFLWLAMLGSVVALRRGEHMRMTAFVNRAQPA
                     TRAFLDLVAIAAALAFLLLILGPSWEYASEERYVTTAALEIPNIWRAAAMPVGAALMI
                     LLTLLRLIDQANWRTTLKALALVAVIVAALVALRPVFAGLGNINLLIFFVGVVAAAVF
                     AGVPIAFAFGLATFGHLALTTDVPMMIVVSRMDEGMSHLILLAVPLFVFLGLLIEMTD
                     MARRMVAFLASLLGHVRGGLSYVLIGAMYLVSGISGAKAADMAAVAPVLFPEMQKRGA
                     NEGELVALLSATGAQTETIPPSLVLITIGSVTGVSITALFTGGLLPGVVLGLMLCLVV
                     HWRCRREDLSTVQKAEGKAISRAFFVALPALALPFVIRASVVEGVATATEVSTIGIVY
                     AMLIGLLVYRHFDFKRLGPMLVNTAALSGSILLIIGAATGMAWALTQSGFSQQLTALM
                     QGLPGGAAAFMAVSIVAFIILGSVLEGIPAIVLFGPLLFPIARAMGIHEVHYAMVVIL
                     SMGLGLFAPPFGVGYYAACAISRVTPDAGIRPIIGYLVTIAIGIVIVAAVPWLSVGFL
                     "
     gene            complement(5401..6408)
                     /locus_tag="VGC69_19485"
     CDS             complement(5401..6408)
                     /locus_tag="VGC69_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY0297518.1"
                     /translation="MNPTRRKLMAGGGAILALAPFQRTLAAPEFRYKYANNLPPTHPM
                     NLRANEAAANIARDTNGRFELAVFPSSQLGSDTDTLSQLRAGAVEFFTLSGLILSTLV
                     PAASISGIGFAFPDYGTVWKGMDGDLGAFIRSEIEGKGLVVMDKIWDNGFRQVTTSSK
                     PINGPDDFRDMKIRVPVSPLWTSMFKALGAAPASINFNEVYSSLQTKIVDAQENPLAI
                     IQTAKLYEVQKYCSMTNHMWDGFWFLGNRRAWEKLPKDIQAVVARHVNAAAMAERDDV
                     AALDKQLQGKLADAGLVFNNTEANPIRDALRKAGFYTEWKGKYGDKAWGLLEKSVGQL
                     S"
     gene            6665..7540
                     /locus_tag="VGC69_19490"
     CDS             6665..7540
                     /locus_tag="VGC69_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0297519.1"
                     /translation="MSGGTARVAGAQAISRAMRVLRAVARMRGEGAGLLALTRDAGMS
                     KPTVHRLLAALMAEGMVEQDPDTRKYFLGPECHVLGNLAGERHGMNHAAADVVARLAR
                     QCGDSAFFSLRSDVFAVCMLREEGDYPLKTYVLMPGDRHPLGVGAGSLAILSALSDDE
                     VEECLRANAALLEERYPNYSPGFLREQVAESRERGYAVNRGRVVAGSWGLGVPLRDAA
                     GRVLGALSIAAVESRLDAERQPQLARLLWREAQRLQDMDSKTTTRRQTCPSEQSSWDG
                     PTFLSANSSSQTLKA"
     gene            7462..8634
                     /locus_tag="VGC69_19495"
     CDS             7462..8634
                     /locus_tag="VGC69_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY0297520.1"
                     /translation="MSKRAVIVGWSHIPFGKLVEPDTESLMARVSGAALDHAGVPREA
                     VDGIYVGVFNNGFDKQDFQGALVALSEPALAYTPATRLENACATGSAAIHAAMDFIEA
                     GRGRIALVVGAEKMTSQPNPVIGDILLNASYRKEEANVEGGFAGVFAQIARAYFARYG
                     DRSEELARIAAKNHKNGVDNPYAQIRKDLGFDFCNEVSDKNPYVAAPLRRTDCSLVSD
                     GAAALVLADEDTARGLARAVGFRARRHANDIMPLSRRDATSFEGARRAWRQALDDARI
                     DLHDLSLVETHDCFTIAELLEYEAMGLAAPGEGYKVVREGITEKTGRLPVNVSGGLKA
                     KGHPIGATGVSMHVLACMQLMGEAGAMQVPGARLAGVFNMGGAAVANYVSILERRK"
     gene            8631..10283
                     /locus_tag="VGC69_19500"
     CDS             8631..10283
                     /locus_tag="VGC69_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY0297521.1"
                     /translation="MSPAVARQAPLGGVAPVSQRVMNLATFLSQSARRLPERPALACG
                     DTVVTWRELDRRAAALARALSEQGIGKGDRVLMHSRNNLELVEIMFAVFRLGAVWVPT
                     NFRIAPDEAAYLAEASGASAFFCQGEFAEHVRAVVEAVPGLRLLVRIGPGDFGQAEVA
                     ELRNRYLDQPLPPADVTRDDPCWFFFTSGTTGRPKAAVLTHGQMAFVVTSHLCDLMPG
                     TTDADASLVVAPLSHGAGIHLLTQVAHGAASILPEGNRFDPDDIWRLVQQWRVSNMFT
                     VPTIVKMLVEHPSVDAWDHASLRYVIYAGAPMYREDQKRALNKLGKVLVQYFGLGEVT
                     GNITVFPPHLHEAEDGPAARIGTCGFERTGMQVSIQDAAGREVEPGVTGEVCVCGPAV
                     FAGYYDNPQANAKSFRNGWFRTGDLGHMDAQGYVYITGRESDMYISGGSNIYPREIEE
                     KTLAHPAVAEVAVLGVPDPVWGEVGVMVCVLREGQQLEEPALQGWLEGNLARYKLPRR
                     IFFWDALPRSGYGKITKKMVREELAARGCLPLQAQAASTAGA"
     gene            10355..11116
                     /locus_tag="VGC69_19505"
     CDS             10355..11116
                     /locus_tag="VGC69_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0297522.1"
                     /translation="MQAKQEVVLVTGGVSGIGLAVTRGLVDEGWKVLAADISQETLAA
                     AAKALPADRVRCVRMDVCDEQQVGEVLKAHEAFGPLAGLVNSAGTGRDVSFFDTDAEL
                     MRSMLEVNLVGTFNVAKQAALRMRERRQGSIVNIASVSGIRGNMGRTAYGASKGGVIT
                     MTRVMAVELAEHGIRVNVVAPGPVETPLVAQMHTSAARESWLRVVPQRRYAQPEELSG
                     TIGWLMDEARASYVTGQVICVDGGFTAGGMLSQSY"
     gene            11188..11778
                     /locus_tag="VGC69_19510"
     CDS             11188..11778
                     /locus_tag="VGC69_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEY0297523.1"
                     /translation="MQDHAKGGGLPRPRAWTHPGPVQAQRITRCGAARGRHLRIALPP
                     GASLFDGLVQPLAALGIRNAAINILGGEFEQLVFCVAPPDPTGRVAVAYTDPIDAGRA
                     YLLGANATLGSNEAGQPMVHCHGGLRDAAGQLLGGHLLPQACMVGRQELIAFAVSLEG
                     FAVQVGYDPETMISLFQPVALAGAPDRAESSMEAVQ"
     gene            11775..12167
                     /locus_tag="VGC69_19515"
     CDS             11775..12167
                     /locus_tag="VGC69_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY0297524.1"
                     /translation="MNHTIESGKTGRVAYARVAPNEDLVGAVEKFCAAEGFRHAFVRG
                     SLGSLIDACLAGPNGTVQQIAGPAVEILSMGGEVRPGADGDLVAALSGVVADPQGRLY
                     GGPFVAGHNPVLMTFELTLEEWVPDSGV"
     gene            12324..13121
                     /locus_tag="VGC69_19520"
     CDS             12324..13121
                     /locus_tag="VGC69_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0297525.1"
                     /translation="MREAKTLKPWLKTAAFAGGLLAIALSGAQAAELHVMTSGGFTAA
                     YNQLGPRFAQATGNTLDTILGPSMGQAPQAIPNRLANGEPGDVVIMVGYALDDLIKQG
                     KVIPDSRVELADSAIGMVVREGQPKPDIGSVKAFRQTLLKAKSIAYSDSASGVYIERE
                     MFKKLGVESQVKSKAKMVERIPVAGEVAKGTYQLGFQQVSELLPIKGVTFVGKIPASV
                     QSITRYAAGIPVNAPHPQEAKALLQYLASPEARPVVQATGLDPVIAK"
     gene            complement(13108..14007)
                     /locus_tag="VGC69_19525"
     CDS             complement(13108..14007)
                     /locus_tag="VGC69_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0297526.1"
                     /translation="MTLSQIRAFVAVAQYNGFTAAARALGTSHSTLSSQIQSIEREYG
                     GELFHRRSRRVELSALGLGLLPLARRMVTLESDMTALLDDSGALRRGFLKIGAVGPYH
                     VTEMIEAYHELYPGIKLSVALGNSEVVLDELDKYLCDVGVLASWLPDERYYMQPYARY
                     PVIAFVHKNHRFAGRKSIALPELTNEPLLMREPGSTTRKALEDALLEHGVTVQVAMEI
                     GSREALRESVARGLGVGTVSMSEYVPDPRLWPLRIEGDVVSTHIHACCLRERRESRLI
                     ASFFEAVDLCMRNSGAAEPLTSR"
     gene            complement(14129..16342)
                     /locus_tag="VGC69_19530"
     CDS             complement(14129..16342)
                     /locus_tag="VGC69_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEY0297527.1"
                     /translation="MIKINRRNFLRAAAASAGLGAFPPSIQRALAIPANNRTGTIQDI
                     EHVVIFMQENRSFDHYFGMLPGVRGYSDRFTIPMASGKPVWYQNATSSRVLQPFYLNP
                     TAANSNGANVGGAHSWSDQQNAWDQGRMASWPNAKSLDASMGYLLESDLSYHWALAKA
                     FTLCDAYYTSQHCGTFPNRAYMWSGTNGSSVSNYNLINNDGIGYTVSGNTIGPSAIGL
                     TWTTYPERLQAAGISWKVYQRYNDNSNNNQLAPFASYRAGIEAANAQGYPVSTPYSAT
                     LEAISPLIKGVGNTLPTTGPGGQGDYLQAFIDDINAGQLPQVSWIVAPAKQSEHPGSS
                     IPDEGAYWIETVLNALTANPDIWSKTVLLVNYDENDAFFDHVPPPCPPSPNGDGSYAG
                     KSTVDVSNEYYTKSVPPIESAAATPPAIDGRPFGPCVRVPMLVISPWSVGGWVNSQTF
                     DHTSTLRFLEQRFGIQESNISPWRRAVFGDMTNCFDFKNPDNALPALVNAPTLAQANA
                     FFTTQTASGTATPAATLAGATPLPQQPLAVRPSRALPYQLHTSANVDASTGLVWLTCS
                     NTGTQGAVFHVYDRYNLAAIPRRYTVEAGKESFDSWNPAANASPNTGKYSLWVLGPNG
                     YHRLFEGSTAGPGNGASPEVRVCYYHANTAVYLTVMNLGNSPATLTISANAYRTDGPW
                     TYTVNPGMQVEPSWSLADSQGWYDFTLSDGAGFVRRFAGRLETGKDSVSDPAMGV"
     gene            complement(16395..16592)
                     /locus_tag="VGC69_19535"
     CDS             complement(16395..16592)
                     /locus_tag="VGC69_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297528.1"
                     /translation="MKTTAFLRLAAAALCTAALLSGCGGDDNTAPQPPDQTPIVKPPA
                     TPGTDSGACPTCQNSQLDCAP"
     gene            complement(16607..18811)
                     /locus_tag="VGC69_19540"
     CDS             complement(16607..18811)
                     /locus_tag="VGC69_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0297529.1"
                     /translation="MTIPKDPACARPAMQDRQTGRPTHPARGAASLMRACALAVALGA
                     ALAGCGGDNGNAPSSASQPSTAQTAGTGTGAGHTLLIELDGATYSAVQTGIANGSLPN
                     LATLQIQVAYSGGVSGALTQQANLATPGWATLLTGTWANRHMVSSDAAGQVLRASSLF
                     DRIKTANAGLNGVAVASPGLAALLAPQKSTGSLDALDDCSTSSTADDCVTSQAAALID
                     GSYAAVVAQYHSAADAAFNFGLGSPQYAATLKQLDTNVGALLAETAKSSGRWLVALTA
                     GYGLNAAGNADGLPLLAESTTFIGLNQAGNDPAGVAAVPSTLNNLYSYASIADITPTL
                     LAWLHAMPDAASYAMDGGELIGDLPAANINVAFNTATTSAALTWQAPPSGDIAVLRNG
                     QQLATLPAGTTTYTDTGLGQYLATAGNQQLNYTVQAGAGTALRATLTPETAFIPLATT
                     LSNGLLAYYPFSNSLPPQDKLGNTTLGPTRADQPATTGTPVAGPFDSGYGLLIDENQR
                     DAGNYEAYMLTFNSASQDITNTAAPSYTVGFWFKVPQNMCITRGDYPLFSNKNFASGA
                     NPGVAIAIYNNGASGIGCTIHFNMGDGSHRVDPAASFSNQQWVYVAEAFDGPNNALYT
                     YVWDPVFGLRSATTSTSALTPLSRLYNAPGGPITVTSGATTYSGAYMGWGLATDGSGQ
                     YYTEQLTAGNKPLDTTYQTAFADLAIWNRVLTANEVQSLYQSRQALSTLVGN"
     gene            complement(19000..>19401)
                     /locus_tag="VGC69_19545"
     CDS             complement(19000..>19401)
                     /locus_tag="VGC69_19545"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0297530.1"
                     /translation="NSTEQTWRTLEDLRRQKIRLAIDDFGTGYSALSYLAKLPLDLLK
                     IDRSFVLTIDAAQDTTVTLDAIIQLGHSLDVQLVAEGVDSPTQFDYLAARGVMFIQGY
                     LFARPMPSAQFAEWYAANGARMAPQQAAAGA"
BASE COUNT         2983 a         6088 c         7031 g         3299 t
ORIGIN      
        1 gcccaccttc aagctcacct tcaagcccga cggcaccttc gagccggcgc gcctcgaagt
       61 gccggccggc cggttcaaga tcgacctggt caatgccagc aaggagccgg tggaattcga
      121 aagcattccc ctgcgcaagg aaaaggtact gggcccgggc gtggagtcct tcgtcgtcat
      181 taccatctcg cggcccggcg aatacccctt cttcgatgat ttccatccta acgtgaaggg
      241 cactttggtg gtcaagtaga acgggccgca tcggttatgg aacaggttct tttcatcgtc
      301 tggcgcgaat gcgtcgaagc cctgctggtg gtgggcattc tgtattcgtg gctgaaggcc
      361 agcccggaag gccgccgcgg cctgccctat ctgtggggcg gcgtggcggg cggcctggcc
      421 ctggccctgg ccctggccct ggtgctgctg ggcgtgtccg agtggctgag cgacacgggg
      481 caggagtggt tccagggcat catgtcgctg gtcgcctgcg cgctggtggt gcagatggtg
      541 gtgtggatga agcagcacgg ccgcaccctc aagcgcgaac tggaaaccgg cgccagggat
      601 tccatcgcgc gcgacaactg gtggggcgtc ttcacgctgg tggcgatcgc cgtggcgcgc
      661 gaaggcagcg agacggtggt gtttctgtac ggcacgattt ccgccgcggg ttcgggcgcg
      721 gccggcggac agtccttcgc ggccttgacc ctggccggcg tggccggcct ggccgcggcg
      781 gggctgacgt tctggctgct gcagctgggc ggcaagttga tcacctggcg gcgtttcttc
      841 aaactgaccg agatcctgct gctcttgctg gccggttcgc tgctggtcgg cggactggac
      901 cacctgatct cgctggacgt catgcccacg ctgatggatc cggtctggga cagttcctgg
      961 ctgttgagcg acagcgccgg cctgggcaag atcctggccg atttcgcggg ctaccgcgcc
     1021 tatccggcct tgagttcgct gctgtgctgg ctggcctact gggtggtggt ctgggcgctg
     1081 ctgcgccggg tgggacgcca gtcgggggcg ccggcccggc aggtggcaca atagtatctt
     1141 tcgcttcgtg cagtgagtga cacaagggtt cagtccatga ctgccgtgat cgggcgcgcc
     1201 agcgtccgct tcgccgattt tctacgcgac catgcaccgc tgctgcgcaa gctgcagtgg
     1261 gggatcgtcg ctgtctacgc attcctgctg gccgtgccgg ccgccatgcc gctgccgggc
     1321 aacacggcct cggtgttcaa caacctgacc atcgccgcgc agttcgcctt ctgggggatc
     1381 tggtggccct tcgtgctggt gtccatgccg gtcctggggc gcgcctggtg cggctggctg
     1441 tgccccgaag gcatgctcac cgaatgggcc agcgagcacg gccgcggcca tgccatcccg
     1501 aagtggatgc gctggggcgg ctggcccttc gtggccttcg cgctcaccac ggtgtacggg
     1561 cagctcatca gcgtctatca atatccgctc gccgtgcttg tcgtgctggg cggctcgacc
     1621 gcgggggcca tggccgtggg ctggtattac ggccacagca agcgcgtgtg gtgcaaatac
     1681 ctgtgcccgg tcaacggcgt gttctcgttg ctggccaagc tggcgccctg gcattacaag
     1741 gtcagcgaag acgcctggcg gcagtccttg tcgacggagc ggcggcgggt gatacgcatc
     1801 gagtccatca attgcgcgcc gctggtgcca ttgcgccaca tgaagggcgc gagcgattgc
     1861 catatgtgcg gccgctgcag cggctaccgc gacgccatcg cgctgacgcc gcgctcgccc
     1921 gaggccgaga tcgtcgaggt cgctcagggc gatgcctggc agacggcgct gatctgcctg
     1981 ggcctgatgg gcatcgcggt gggcgccttc ctgtggagcg cgagcccttg gtacgtggac
     2041 ctgaagcagc tcatcgccac atggctgatc gaccacaatg tgatgtggcc gctggccgac
     2101 aatgcgcctt ggttcattct gacgcactat cccgaggtca acgacagctt ctcgtggctg
     2161 gatggtgcgc tgatcctggt gttcgcgatg ggcgccgcgg ccgtggtggg cggctcgctc
     2221 tacctggccc tgtggatagc cgaccgcttg gcgccgatgc agggcgcggc cggccgtttt
     2281 gccggcctgc acaagctggc ccaggccttg atcccttccg cgggcgtcgg cgtgttcctg
     2341 gggctgtcgg ccaccaccat taccctgctg cagcatgagg gcatggcgac gggctgggtg
     2401 cggcccgtgc gattcaccct gctggcgctg gcgctggcct ggtcgctgcg cctggcttgg
     2461 cgcctgctgg ggcggcggac tgccggcgcg ggtcgccgcg ccttggcgtg gctggcattc
     2521 gccgccgggc tcgcgccctt cgcggcggcc tgggtgctgt tcttcgctgt ctggtagttt
     2581 ttcgcgcgat ctgttccggt gcggtggcgg cggcatgcgc taaggtggtt gcccgctgac
     2641 atcccggaaa gccgccatgc cgtcctatac ctttcacctc gtcaacgtct ttgccgaatc
     2701 cactttcggc ggcaatccct tgtgcgtgtt cgatgacgcg cggggcatgg acgaggccgc
     2761 catgcagagc ctggcgctgc agttcaatct ttccgagacg accttcgtgc tgccctcggg
     2821 cgaggcggat gcgcaggtgc gcatcttcac gccgggttac gagatgcgct ttgccgggca
     2881 tcccacgctg ggcacggcgc acgtggtgcg cggcgtccgc ggcctgggca atgatctgac
     2941 gctgcaattc aaggcgggat cggtgccggt gcgggcgcag ggcgacctct ggacgctggt
     3001 gccgcccctg gacggcgcgc cgcgtacggc gccgcccacg caatctcctg ccgaaatcgc
     3061 ggccctgctg ggcctgtcgc cggacgactt gctggaaacc ccgctgtggg tcgacacggg
     3121 cgccgaccag ttgctggtgc cggtgaagac ggccgatgcc gtgcgccgcg cgcagccgga
     3181 cgcggcgcgg ctagcgcaat ggccggccag cagcctgggg cacaagacgg cctatgtctt
     3241 cgccttcacc ggcgacaggc aggacgggcg cgacgtggtg ctgtcgcgct atttcttttc
     3301 caaggaaggc ggcggcgtgg cggaggatcc gggcacgggg tcggcctgcg ccaacctggg
     3361 cggctggctg ctggcgcgcg gccgcgcctt gccgtccagc atcctggtgc gccagggcga
     3421 gcaggccggg cggccgtgca cgctatacct ggacgtggac caggacggca cgatccgcgt
     3481 aggcggccgc gtcatcgcgc tgggcaaggg cgagatcgcg ctatagaaac ccgaccgaca
     3541 gccagggaac ggccgccacg atcacgatgc cgatggcgat cgtgaccagg tatccgatga
     3601 tgggccggat gccggcgtcc ggcgttacgc ggctgatggc gcaggcggcg tagtagccca
     3661 ccccgaacgg cggggcgaac aggcccagcc ccatggagag gatcaccacc atcgcgtaat
     3721 gcacctcgtg gatgcccatg gcgcgggcga tgggaaacag cagggggccg aacagcacga
     3781 tggcgggtat cccctcgagc acgctgccca ggatgatgaa ggccacgatg gacaccgcca
     3841 tgaaggcggc cgcgccgccc ggcaggccct gcatcagggc cgtgagttgc tgcgagaagc
     3901 ccgattgcgt cagggcccag gccatgcccg tggccgcgcc gatgatgagc aggatggagc
     3961 ccgacagcgc cgccgtgttc accagcatgg ggcccagccg cttgaagtcg aagtggcggt
     4021 agaccagcag gccgatcagc atggcgtaga cgatgccgat ggtcgagacc tcggtggcgg
     4081 tggccacgcc ttcgaccacg gaagcgcgga tgacgaaggg cagggccagc gcgggcaggg
     4141 ccacgaagaa ggcgcgggag atggccttgc cttcggcctt ttgcacggtg gagaggtctt
     4201 cgcgccggca gcgccagtgc accaccaggc acagcatcag gcccagcacc acgccgggca
     4261 gcaagccgcc ggtaaagagc gccgtgatcg acacgccggt gaccgagccg atggtgatga
     4321 gcaccaggct gggcggaatg gtttcggtct gcgcgccggt ggccgagagc agggccacca
     4381 gttcgccttc gttggcgccg cgcttttgca tctcgggaaa cagcaccggc gccaccgcgg
     4441 ccatgtcggc ggccttggcg cccgagatgc ccgacaccag gtacatggcg ccgatcagca
     4501 catacgacag gccgccgcgc acgtggccca gcaggctggc gagaaaggcc accatgcggc
     4561 gggccatgtc cgtcatctcg atcaggaggc cgaggaagac gaagagcggc acggccagca
     4621 ggatcagatg cgacatgcct tcgtccatgc ggctgaccac gatcatcatg ggcacatcgg
     4681 tggtcagcgc caggtggccg aaggtggcca ggccgaaggc gaaggcaatg ggcacgccgg
     4741 caaagaccgc ggccgccacc acgccgacga agaagatgag caggttgata ttgcccaggc
     4801 cggcgaacac gggccgcagc gcgaccagcg cggccacgat cacggccacc agcgccagcg
     4861 ccttcagcgt ggtgcgccag ttggcctggt cgatcaggcg cagcaaggtg aggaggatca
     4921 tcaacgcggc gccgaccggc atggccgcgg cccgccagat attggggatc tcgagcgcgg
     4981 cggtggtgac gtagcgctct tccgaggcgt attcccagct ggggcccagg atgagcagca
     5041 aaaaggccag ggccgccgcg atggccacca ggtcgaggaa ggcgcgggtt gccggctgcg
     5101 cgcggttgac gaaagccgtc atgcgcatgt gctcgccgcg ccgcagggcc acgaccgacc
     5161 ccagcatggc cagccacagg aagaggatgg aggcgagctc gtcggaccag accagcggat
     5221 tgcggaacac atagcgcgcc acgatgccgg tgaacaggat gacgacctcg gcggccacga
     5281 ggatggccgc cgggatttcc accaaccgac ccagcagccc gtcggccacc gccgtccagg
     5341 ggcggtggcc gacgggccgg gacttgggca gcccggccgc gtgggagtcg gccatgacgc
     5401 tcacgacaac tgcccgacgg atttctccag caggccccag gctttgtcgc cgtactttcc
     5461 tttccattcg gtatagaaac cggccttgcg cagcgcgtcg cggatgggat tggcctcggt
     5521 gttgttgaag accaggccgg cgtcggccag cttgccctgc aactgcttgt ccagcgcggc
     5581 cacgtcgtcg cgctcggcca tggccgcggc gttgacgtgc ctggccacca cggcctggat
     5641 gtccttgggc agcttttccc aggcccgccg gttgcccagg aaccagaagc cgtcccacat
     5701 gtggttggtc atcgagcagt acttctgcac ctcgtacagc ttggcggtct ggatgatggc
     5761 cagcgggttt tcctgcgcgt cgacgatctt ggtctgcagc gaggaataga cctcgttgaa
     5821 gttgatgctg gccggcgcgg cgcccagcgc cttgaacatg gacgtccaca gcgggctgac
     5881 cggcacgcgg atcttcatgt cgcggaaatc gtcgggaccg ttgatgggct tgctgctggt
     5941 cgtgacctgg cggaatccgt tgtcccagat cttgtccatc accaccaggc ccttgccctc
     6001 gatctccgag cggatgaatg cgccgagatc gccgtccatg cccttccaga ccgtgccgta
     6061 gtcggggaag gcaaaaccga tgccgctgat cgaggccgcg ggcaccagcg tggagagaat
     6121 caggcccgac agggtgaaga actcgaccgc gcccgcgcgc aactggctca gggtgtcggt
     6181 gtcggagccg agctggctgc tggggaagac ggcgagctcg aagcggccgt tggtgtcgcg
     6241 cgcgatattg gccgcggcct cgttggcgcg caggttcatc gggtgcgtgg gcggcaggtt
     6301 gttggcgtac ttgtagcgga attcgggggc cgccaaagta cgttggaagg gggcgagcgc
     6361 caaaatggca ccgccgcccg ccatgagctt gcgtcgagtc gggttcatgc tgtctcctgg
     6421 tgtattgccc tctgggttta ttccctatgc gcgggctgtt ttgaatgcat gatatgcctg
     6481 cgattggcgc cgcagggtgg tctacatggt agacctatga atcggctctt gaagctattc
     6541 aaacatatcg tttattctgt gtaaagcctt tctgatttcg tctatttcga accagaaaga
     6601 atcacagaaa gaatcatcca tggtctacac aatggaccat ttggcggacg ccgaggaagg
     6661 ctgcatgtct ggtggaacgg cccgggttgc aggggcacag gctatctcgc gggccatgcg
     6721 cgtgctgcgc gccgtggcgc gcatgcgtgg cgagggcgcg ggcctcttgg cgctcacgcg
     6781 cgacgcgggc atgagcaagc ccacggtgca tcgattgctg gccgcgctca tggccgaggg
     6841 catggtcgag caggacccgg atacgcgcaa atacttcctg ggtcccgaat gccacgtgct
     6901 gggcaatctg gcgggcgagc gccacggcat gaaccatgcc gcggccgacg tcgtggcccg
     6961 cctggcgcgc cagtgcggcg attcggcctt cttttcgctg cgcagcgacg tgttcgccgt
     7021 ctgcatgctg cgcgaggagg gcgactatcc gctcaagacg tacgtgctca tgccgggcga
     7081 ccggcacccg ctgggggtgg gcgcgggcag cctggccatt ctgtcggcct tgtccgacga
     7141 cgaggtcgaa gaatgcctgc gggccaacgc ggccttgctc gaggagcggt atcccaatta
     7201 ttcacccggc ttcctgcgcg agcaggtggc cgagtcgcgt gagcgcggct atgcggtcaa
     7261 tcgcggccgc gtcgtcgcgg gttcgtgggg gctgggcgtg ccattgcgcg acgccgccgg
     7321 ccgcgtgctg ggcgcgctga gcatcgcggc ggtcgaaagc cgcctcgatg ccgagcgcca
     7381 gccgcagctg gcccgtctgc tgtggcgcga ggcccagcgc ctgcaagaca tggattccaa
     7441 aacaaccacc aggagacaaa catgtccaag cgagcagtca tcgtgggatg gtcccacatt
     7501 cctttcggca aactcgtcga gccagacact gaaagcctga tggcccgcgt gtcgggcgcg
     7561 gcgctcgacc atgccggcgt gccgcgcgag gcggtcgacg gcatttacgt cggcgtgttc
     7621 aacaacggtt tcgacaagca ggacttccag ggcgcgctgg tggcgctgtc cgagcccgcg
     7681 ctggcctaca cgccggccac ccggttggag aacgcctgcg ccacgggctc ggcggccatc
     7741 catgccgcca tggacttcat cgaggccggg cgcggccgca tcgccctggt ggtcggcgcc
     7801 gagaaaatga cctcgcagcc caatccggtg atcggcgaca tcctgctcaa cgccagctac
     7861 cgcaaggaag aggcgaatgt cgagggcggc ttcgccggcg tgttcgccca gatcgcccgc
     7921 gcctatttcg cgcgctacgg cgatcgcagc gaggagctgg cccgcatcgc cgccaagaac
     7981 cacaagaacg gggtggacaa tccctatgcg cagatccgca aggacctggg cttcgacttc
     8041 tgcaacgagg tgtcggacaa gaacccctat gtggccgcgc ccctgcgccg cacggattgc
     8101 tcgctggtgt ccgacggcgc cgcggccttg gtgctggccg acgaggacac ggcccggggc
     8161 ctggcgcgcg ccgtgggctt tcgggcgcgc cgccatgcca acgacatcat gccgctgtcg
     8221 cggcgcgacg ccacttcctt cgagggcgcg cggcgcgcct ggcggcaagc cctggacgat
     8281 gccaggatcg atctgcatga cctgagcctg gtcgaaacgc acgactgctt caccatcgcc
     8341 gagctgctcg agtacgaggc catgggcctg gccgcgccgg gcgagggcta caaagtggtg
     8401 cgcgagggca tcaccgagaa gaccggccgc ctgcccgtga acgtgtcggg cgggctcaag
     8461 gccaaggggc atcccatcgg cgcgacgggc gtgtccatgc acgtgctggc ctgcatgcag
     8521 ctgatgggcg aggccggcgc catgcaggtg cccggcgcca ggctggcggg cgtgttcaat
     8581 atgggcggcg cggcggtggc caactacgtc agcatcctgg agcgccgcaa atgagcccgg
     8641 ccgtggctcg gcaagcgccg ctgggcggcg tggccccggt atcgcagcgg gtgatgaacc
     8701 tggccacttt cctgtcgcag tccgcgcgcc gcctgcccga gcggcccgcg ctggcatgcg
     8761 gcgataccgt cgtgacctgg cgcgaactgg accggcgcgc cgccgcgctg gcgcgcgcct
     8821 tgtccgaaca gggcatcggc aagggcgacc gcgtgctcat gcactcgcgc aacaatctcg
     8881 agctggtcga gatcatgttc gcggtgttcc ggctgggggc ggtatgggtg cccaccaact
     8941 ttcgcatcgc gcccgacgag gcggcctacc tggccgaggc ctcgggcgca tcggcctttt
     9001 tctgccaggg cgagtttgcc gagcatgtgc gcgccgtggt cgaggcggtg cctggcctgc
     9061 gcttgctggt gcgcatcggg ccgggcgatt tcggccaggc cgaggtggcc gaactgcgca
     9121 accgctatct cgaccagccc ttgccgccgg ccgatgtgac gcgcgacgac ccgtgctggt
     9181 tcttcttcac ctcgggcacg acggggcggc ccaaggccgc cgtgctcacg cacggccaga
     9241 tggcctttgt ggtcaccagc catttgtgcg acttgatgcc cggcaccacc gacgccgatg
     9301 cgagcctggt ggtggcgccg ctttcgcacg gggccggcat ccatctgctg acgcaggtgg
     9361 cgcacggggc ggcgagcatc ctgcccgaag gcaaccggtt cgatcccgac gacatctggc
     9421 gcctggtgca gcagtggcgg gtcagcaata tgttcacggt gcccaccatc gtcaagatgc
     9481 tggtggagca tccgtcggtc gacgcgtggg accacgccag cctgcgctat gtgatctatg
     9541 cgggcgcgcc catgtaccgc gaagaccaga agcgggccct gaacaagctg ggcaaggtgc
     9601 tggtgcagta cttcgggctg ggcgaggtga cgggcaatat caccgtgttt ccgccgcacc
     9661 tgcacgaggc cgaggacggc cccgcggcgc gcatcggcac ctgcggtttc gaacgcacgg
     9721 gcatgcaggt ctcgatacag gatgccgcag gccgcgaggt cgagccgggc gtcacgggag
     9781 aggtgtgcgt atgcggcccg gccgtgttcg ccggctacta cgacaaccca caggccaatg
     9841 ccaagtcgtt tcgcaacggc tggttccgca ccggcgacct gggccacatg gatgcgcaag
     9901 ggtatgtcta catcaccggg cgcgagtccg atatgtacat atcgggcggc tccaacatct
     9961 acccgcgcga gatcgaggaa aagaccctgg cgcatccggc cgtcgccgaa gtggccgtgc
    10021 tgggcgtgcc cgatccggta tggggcgagg tgggcgtgat ggtctgcgtg ctgcgcgaag
    10081 gccagcagct cgaagagcct gcgctgcagg gctggctgga aggcaatctg gcccgctaca
    10141 aattgccgcg gcgcattttc ttctgggacg ccttgccgcg ttcgggctac ggcaagatca
    10201 ccaagaagat ggtgcgcgaa gaactggccg cgcgcggatg cctgcccttg caggcacagg
    10261 ccgcgagcac cgcaggcgcc tgatttcagg ccaggcaagt cccggcgaca cggcacgcag
    10321 aggccgtgcg ccggcaaaac agaggagaca aaccatgcaa gccaagcagg aagtcgtact
    10381 ggtgacaggc ggcgtatcgg gtatcgggct ggccgtgacg cgcggcctgg tcgacgaagg
    10441 gtggaaagtg ctggcggccg acatctcgca ggagacgctg gccgccgcgg ccaaggcgct
    10501 gccggcggac cgcgtgcgct gtgtgcggat ggacgtgtgc gacgaacagc aggtcggcga
    10561 ggtgttgaag gcccacgagg ccttcggacc gctggccggc ctggtcaatt cggccggcac
    10621 cggccgcgac gtgtccttct tcgacaccga cgccgagctc atgcgcagca tgctcgaggt
    10681 caatctggtc ggcaccttca atgtggccaa gcaggcggcc ctgcgcatgc gcgagcgccg
    10741 gcagggctcc atcgtcaaca tcgcgtcggt gtcgggcata cgcggcaata tgggccgcac
    10801 cgcctacggc gcgtccaagg gcggcgtcat caccatgacg cgcgtcatgg ctgtggagct
    10861 ggccgagcat ggcatccgcg tcaacgtcgt ggcgccgggt ccggtggaga cgccgctggt
    10921 ggcccagatg catacctcgg ccgcgcgcga gtcctggctg cgcgtggtgc cgcagcggcg
    10981 ctatgcccag cccgaggaat tgagcggcac cataggctgg ctgatggacg aggcccgcgc
    11041 cagttatgta acaggacagg tgatctgtgt tgacggcggc tttaccgccg gcggtatgct
    11101 gagccaaagc tattaaaagc agtcccaccc cggcagccgg cctacccgtg ccgactcgcc
    11161 ggttcgccat aagcgaggag acacatcatg caagaccatg caaaaggggg cggcctgccg
    11221 cgccccaggg cctggacgca tcccgggccg gtacaggcgc agcgcattac ccgctgcggc
    11281 gcggcgcgag ggcgccatct ccgcatcgcg ctgccgccgg gcgcaagcct gttcgacggc
    11341 ctggtgcagc cgctggcggc gctgggcata cgcaatgccg cgatcaatat cctgggcggt
    11401 gaattcgagc aactggtctt ctgcgtggcg ccgcccgatc cgaccgggcg cgtggccgtg
    11461 gcctataccg atcccatcga tgcgggccgg gcctatctgc tgggagccaa tgccaccctg
    11521 ggcagcaacg aggccgggca gcccatggtg cactgccacg gcgggctgcg cgacgcggcg
    11581 gggcaattgc tcggcgggca tctgctgccg caggcctgca tggtcggccg gcaggaactg
    11641 atcgcgtttg ccgtgtccct ggaagggttc gccgtgcagg tcggctacga cccggagacc
    11701 atgatttccc tgttccagcc ggtggcgctg gcaggcgcgc cggatcgcgc cgagtcgtcc
    11761 atggaggccg tgcaatgaac cacaccatcg aaagcggcaa gaccggccgc gtggcttacg
    11821 cgcgggtggc gcccaacgag gatctggtcg gcgccgtgga gaagttctgc gcggcggaag
    11881 gctttcgcca tgccttcgtg cgcggctcgc tgggcagcct gatcgatgcc tgcctggccg
    11941 gcccgaacgg cacggtgcag cagatcgccg gcccggctgt cgagatactg agcatggggg
    12001 gcgaggtgcg cccgggagcc gacggcgacc tggttgcggc gctatcgggc gtggtggccg
    12061 acccgcaggg caggctctac ggcgggccgt tcgtggcggg gcacaacccg gtgctgatga
    12121 ccttcgagct cacgctggaa gagtgggtgc cggatagcgg ggtttagcca ggcgatcttg
    12181 ctctcttccc aggggagtat cgtcaaggcc atgcaggccc gcgcttgagc gatgcctgct
    12241 tgccgggagg ggtattcatc ccattgacgg gttccgccgt gtaagctcgc cctcccttta
    12301 agctgcaata aaaggagact cccatgcgag aagcaaagac cctgaaaccc tggttgaaaa
    12361 ccgccgcctt cgccggcggc ctgcttgcca tcgcgctgag cggcgcgcag gcggccgagc
    12421 tgcacgtgat gacttccggc ggattcaccg ccgcgtacaa ccagctcggc ccacgctttg
    12481 cccaggccac gggcaatacg ctcgacacta tcctcggccc gtcgatgggc caagcgcccc
    12541 aggcgattcc caaccggctc gccaacggcg aacccggcga cgtggtcatc atggtcggct
    12601 atgcgctcga cgacctgatc aagcagggca aggtcatacc cgactcgcgc gtcgaactgg
    12661 ccgattccgc catcggcatg gtggtgcgcg aaggccagcc caagccggac atcggctcgg
    12721 tcaaggcgtt ccgccagacc ctgctcaagg ccaagtccat cgcctattcc gacagcgcga
    12781 gcggcgtcta catcgaacgc gagatgttca agaagctcgg cgtcgaaagc caggtcaagt
    12841 ccaaggccaa gatggtcgag cgcatcccgg tcgccggcga agtggccaag ggcacctacc
    12901 agctcggctt ccagcaggtc agcgagctgt tgccgatcaa gggcgtgacc ttcgtcggca
    12961 agatcccggc ctccgttcag tcgatcacgc gctatgcggc cggcatcccg gtcaatgcgc
    13021 cgcatccgca agaagcgaag gccttgctcc agtatctcgc ctcgcccgag gcccgccctg
    13081 tcgtgcaagc aaccggcctg gatccggtca tcgcgaagta agcggttcgg ccgcgcccga
    13141 gttgcgcatg cacaggtcga cggcttcgaa gaacgaggcg atcagccggc tttcccgccg
    13201 ctcgcgcagg cagcaggcgt ggatgtgcgt cgagacgacg tcgccttcga tgcgcaacgg
    13261 ccacagccgc gggtccggca cgtattccga catcgagacc gtgcccacgc ccagcccgcg
    13321 cgcgaccgat tcccgcaggg cttcgcggct gccgatctcc atggcgacct gcacggtcac
    13381 gccgtgttcc agcagggcgt cctcgagcgc cttgcgcgtg gtggagccgg gttcgcgcat
    13441 cagcagcggc tcgttggtca gttccggcag ggcgatggat ttccggcccg cgaaacgatg
    13501 gttcttgtgc acgaaggcga tcaccggata gcgcgcatag ggctgcatgt aatagcgttc
    13561 gtccggcagc cagctggcca gcacccccac gtcgcaaagg tatttgtcga gctcgtccag
    13621 cacgacctcc gagttgccga gcgccaccga cagcttgatg cccggataga gctcgtggta
    13681 ggcctcgatc atctcggtga cgtggtaggg gccgaccgcg ccgatcttca ggaaaccccg
    13741 gcgcagcgcg ccgctgtcgt ccagcagcgc ggtcatgtcg ctttcgagcg tgaccatgcg
    13801 gcgcgcgagc ggcagcagtc ccaggcccag cgccgagagc tcgacgcgcc ggctgcgccg
    13861 gtggaagagc tcgccgccgt actcgcgctc gatcgactgg atctgcgaac tgagggtgct
    13921 gtgactggtg ccgagcgcgc gcgcggcggc ggtgaagccg ttgtactgcg cgacggcgac
    13981 gaaggcgcgg atctggctga gcgtcatgtg ggcctgcgcc gggcgaggct cggcagggtg
    14041 atgaaggcga agtcttcatt gtcgaatcgg catgtgacca tcgcatttcc ggcccgcgcc
    14101 cggtctgcgc cggggcgcgg gccgggattc agacgcccat ggccgggtcg ctgacgctgt
    14161 ccttgcccgt ttcgagccgg ccggcgaagc gccgcacgaa gcccgcgccg tcgctcagcg
    14221 tgaagtcata ccagccctgc gagtcggcca ggctccagga gggctccact tgcatgccgg
    14281 ggttgacggt ataggtccag gggccgtcgg tgcggtaggc gttggcggag atggtgagcg
    14341 tggctggcga attgcccagg ttcatgaccg tcaggtagac cgcggtgttg gcgtggtagt
    14401 agcagacgcg cacctccggg ctggcgccgt tgcccggccc ggccgtgctg ccctcgaaca
    14461 gccggtggta gccgttgggg ccgagcaccc agaggctgta cttgcccgtg ttgggagagg
    14521 cgttggcggc ggggttccac gaatcgaacg attccttgcc ggcctcgacc gtgtagcggc
    14581 gcgggatcgc cgcgaggttg tagcggtcgt agacgtggaa tacggccccc tgcgtgccgg
    14641 tattggagca ggtcagccag accagcccgg tgctcgcgtc cacgttggcg ctggtgtgca
    14701 actgataggg cagcgcgcgc gagggccgga ccgccagggg ctgctgcggc aggggggtcg
    14761 cgccggcgag ggtcgcggcg ggcgtggcgg ttccgctggc ggtctgcgtg gtgaagaatg
    14821 cgttggcctg cgccagggtc ggcgcattga ccagcgcggg cagggcgttg tcggggttct
    14881 tgaagtcgaa gcagttggtc atgtcgccga acacggcgcg gcgccagggg ctgatattgg
    14941 actcctggat gccgaagcgc tgttccagga agcgcagcgt cgaggtgtgg tcgaaggtct
    15001 gcgagttgac ccagccgccc acgctccagg gcgagatcac cagcatgggc acgcgcacgc
    15061 aggggccgaa gggccggccg tcgatggcgg gcggggtggc cgccgccgac tcgatcggcg
    15121 gcacggactt ggtgtagtac tcgttggaca cgtccacggt ggacttgccc gcgtagctgc
    15181 cgtcgccgtt gggcgagggc gggcacggcg gcggcacgtg gtcgaagaag gcgtcgttct
    15241 cgtcatagtt gaccagcagc accgtcttgc tccagatgtc cgggttggcg gtcagcgcat
    15301 tgaggacggt ctctatccaa taggcgccct cgtccgggat ggagctgccg ggatgttcgc
    15361 tttgcttggc aggagccacg atccagctga cctgcggcaa ttggccggcg ttgatgtcgt
    15421 cgatgaatgc ctgcaggtag tcgccctgtc cgcccgggcc cgtggtgggc agcgtattgc
    15481 ccacgccctt gatgagcggc gagatggcct ccagcgtggc cgagtagggc gtgctgaccg
    15541 ggtagccttg cgcgttcgcg gcttcgatgc ctgcgcggta gctggcgaag ggagccagct
    15601 ggttgttgtt gctattgtcg ttgtagcgct ggtagacctt ccagctgatg ccggcggcct
    15661 gcaggcgctc ggggtaggtg gtccaggtca ggccgatggc ggagggaccg atcgtgttgc
    15721 ccgagacggt gtagccgata ccgtcgttgt tgatcaggtt gtagttgctc acgctggagc
    15781 cattggtgcc gctccacata taggcccggt tcgggaaggt gccgcagtgc tgcgaggtgt
    15841 agtaggcgtc gcagagggtg aaggccttgg ccagggccca gtggtaggac aggtcggact
    15901 ccagcaaata gcccatcgag gcgtccaggc tcttggcgtt gggccaggac gccatgcggc
    15961 cctggtccca ggcattttgc tggtcggacc aggagtgcgc cccgccgacg ttggcgccat
    16021 tgctgttggc ggccgtgggg ttcaggtaga agggttgcag gacccggctg gacgtcgcgt
    16081 tctggtacca caccggcttg cccgaggcca tggggatggt gaagcggtcg ctgtagccgc
    16141 gcacgcccgg cagcatgccg aaataatggt cgaaggaacg gttctcctgc atgaagatga
    16201 cgacgtgctc gatgtcctgg atggtgccgg tccggttgtt cgcgggaatg gcgagcgccc
    16261 gctggatgga gggcgggaag gcgcccagtc cggcgctggc cgcggcggcg cgcaggaagt
    16321 tgcggcgatt gattttgatc atggtgtagg cggtaggaga cgtggattcg aggcgctggc
    16381 gtcggcccgc ggcgtcaggg cgcgcagtcg agctgcgagt tctggcaggt cgggcaggcg
    16441 cccgagtccg tgcccggggt ggcgggcggc ttgacgatcg gcgtctggtc gggcggctgc
    16501 ggcgcggtgt tgtcgtcgcc gccgcagccc gagagcaggg ccgcggtgca gagggcggcc
    16561 gcggcgagcc gcaggaaggc agtcgttttc atggctgtgc cgctccttag ttgccgacca
    16621 gcgtggacag tgcctgcctg gactggtaga gcgactgcac ctcgttggcg gtcagcacgc
    16681 ggttccatat ggccaggtcg gcgaaggcgg tctggtaggt ggtgtcgaga ggcttgttgc
    16741 cggcggtcag ctgttccgtg tagtactggc ccgagccgtc ggtggcgagg ccccagccca
    16801 tataggcgcc gctataggtc gtcgcgccgc tggtgacggt gatgggaccg cccggcgcgt
    16861 tgtagagcct ggacaggggc gtcagcgcgc tcgtcgaggt ggtggcgctg cgcaggccga
    16921 agacgggatc ccagacatag gtgtagagcg cgttgttcgg gccgtcgaat gcctcggcca
    16981 cgtagaccca ttgctggttg ctgaaggacg ccgcgggatc cacgcggtgg ctgccgtcgc
    17041 ccatgttgaa gtggatggtg cagcctatgc cggaggcgcc gttgttgtag atggcgatcg
    17101 cgacgcccgg gttggcgccg gaggcgaagt tcttgttgga gaacaaggga tagtcgcccc
    17161 gggtaatgca catgttctgc ggaaccttga accagaagcc gacggtgtag gacggcgccg
    17221 cggtgttggt gatgtcctgg ctcgccgaat tgaaggtgag catataggct tcgtagttgc
    17281 cggcgtcgcg ttggttttcg tcgatcagca agccgtagcc gctgtcgaac gggccggcga
    17341 cgggggtgcc ggtcgttgcg ggctggtcgg cccgggtggg gcccagggtg gtgttgccca
    17401 gcttgtcctg gggcggcagg ctgttgctga aggggtagta ggccagcagc ccgttggaca
    17461 gcgtcgtcgc gagcgggatg aacgcggttt ccggcgtgag cgtggcgcgc aaggccgtgc
    17521 ccgcgcccgc ctgcacggtg tagttcaact gctggttgcc ggccgtcgcc aggtattgcc
    17581 cgaggccggt gtcggtgtag gtggtggtgc ccgcgggcag ggtggcgagc tgttggccgt
    17641 tgcgcagcac ggcgatatct ccgctgggcg gcgcttgcca ggtcagcgcg gcgctggtgg
    17701 tggcggtgtt gaacgcgaca ttgatgttgg cagccggcag gtcgccgatc agttcgccgc
    17761 cgtccatggc gtagctggcc gcgtcgggca tggcgtgcag ccaggcgagc agcgtgggcg
    17821 tgatgtcggc gatgctggcg taggaataca ggttgttgag cgtcgacggc acggccgcca
    17881 cgccggcagg gtcgttgccg gcctggttga gcccgatgaa ggtggtcgat tccgccagca
    17941 gcggcaggcc gtcggcgttg ccggccgcgt tcaggccgta gcccgcggtc agcgcgacca
    18001 gccagcggcc gctgcttttc gcggtttcgg ccagcagcgc gccgacgttc gtgtcgagct
    18061 gcttgagcgt ggctgcgtat tgcggggagc cgagcccgaa gttgaatgcc gcgtcggcgg
    18121 cggaatggta ttgcgcgacc acggccgcgt agctgccgtc gatcagcgcg gcggcctggc
    18181 tcgtcacgca gtcgtcggcg gtgctgctcg tcgagcaatc gtcgagcgca tccagcgagc
    18241 ccgtgctttt ttgcggcgcg agcagcgcgg ccagtcccgg cgaggcgacg gccacgccgt
    18301 tcaggccggc attggccgtc ttgatcctgt cgaacagcga actcgcgcgc aggacctgcc
    18361 cggcggcgtc cgaggacacc atgtggcgat tggcccaggt tccggtgagg agagtcgccc
    18421 aacctggcgt ggccaggttg gcctgctgcg tcagcgcgcc cgacacgccg ccgctgtagg
    18481 ccacctggat ctgcagcgtg gcgagattgg gcagggaacc gttggcaatg ccggtctgca
    18541 cggcgctgta ggtggcgccg tcgagttcga tgagcagcgt gtggccggca cccgtgcccg
    18601 tgccggcggt ttgcgcggtg gaaggctggg aagcggacga cggcgcgttg ccgttatcgc
    18661 cgccgcagcc ggccagcgcg gcgccgagcg ccaccgccag cgcacaggcg cgcatcaggc
    18721 tcgccgcgcc gcgggcggga tgtgtcggtc tgccggtttg ccggtcttgc atggcgggcc
    18781 tggcgcaagc ggggtctttc gggatggtca tggtgttcct gcgtgaaatg aggagtctgc
    18841 cggtgggctg cccatgcgtg cctggctggg cgaaggcgta agggcaatgt aaagacgcag
    18901 cgagacatgc ccgtgacgaa agcccgggag atttccgcaa gttctgttga tgttttcgtc
    18961 agacgggggc ccgccgagcg gcgggccctc gcggccgggc taggcgccgg ccgcggcttg
    19021 ctgcggcgcc atgcgcgcgc cgttggccgc gtaccactcg gcgaattgcg ccgaaggcat
    19081 gggccgggca aagagatagc cctggatgaa catcacgccg cgcgcggcca ggtaatcgaa
    19141 ctgcgtcggc gagtccacgc cttcggcgac aagctgcacg tccaggctgt ggccgagctg
    19201 gatgatggca tccagcgtca cggtggtgtc ttgcgcggca tcgatcgtca gcacgaagct
    19261 gcggtcgatt ttcagcaggt cgagagggag cttggccagg tagctcaatg ccgaatagcc
    19321 ggtgccgaaa tcatcgatgg caaggcgtat tttttgccgg cgcaggtctt ccagcgtgcg
    19381 ccaggtctgc tccgtgctgt t
//
LOCUS       DASWPO010000364        12579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2052808, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000364 DASWPO010000000
VERSION     DASWPO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12579
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052808"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGC69_19550"
     CDS             complement(<1..765)
                     /locus_tag="VGC69_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY0297531.1"
                     /translation="MSNSTFAGTYPFAGRQVNRMGYGAMQLAGPGVFGPPKDPAAAVA
                     VLREALAAGVNHIDTSDFYGPHITNRLIKEALHPYPGDLLIVTKVGAVRGDDAAWLAA
                     QEPADIERGVHDNLRNLGLDALDVVNMRIMGNIHVPSEGSIARQVEALAALQQRGLVR
                     HIGLSNASAAQVEEAQGIAEIACVQNHYNLVHRDDDALIDALAEQGIAYVPFFPLGGF
                     TPIQSGELNRIAASVGATPMQLALAWLLRRAPNILLI"
     gene            870..1793
                     /locus_tag="VGC69_19555"
     CDS             870..1793
                     /locus_tag="VGC69_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0297532.1"
                     /translation="MDMSDLAAFVSVAQAGGFRDAARVGGVSASSLSTAVQRLEAKLG
                     MRLLNRTTRSVAPTEAGRRLLEQLAPLFGEIEAALDVLNGFRDRPVGTLRLNVPAGVS
                     HTLLPPILSGFLKAYPEIQVEVVVEDGFVNLLEIGCDAGIRYDERLEQDMIAVPIGPR
                     VQRFATAAAPAYLDARGRPGHPRDLLGHACLRGQFAGGVTPLWHFERDGEVMHIDPPP
                     GPLLVRPGVAITLMVRAAIDGLGIIHLWEDMLRPQLDSGLLAPLLEPWWQRFPGPFLY
                     YPGRRHLPAPLRAFVDFLKTPAPAPRAAPDG"
     gene            complement(1800..3239)
                     /locus_tag="VGC69_19560"
     CDS             complement(1800..3239)
                     /locus_tag="VGC69_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0297533.1"
                     /translation="MTETEYQELDGLALAALLKKKEVSSTQLMEHAIALSRTRGVELN
                     AIRYERYDDALQWARQWQHAGAFQGIPFLLKDSSLASKRLPMSLGSRLFTDTSFTFDA
                     TLTQRFEAAGLIPFARTSVPEFCMAPTTEAVANGAVTINPWDAARSTGGSSGGAAVAV
                     ASRIVPVAHGSDGGGSIRIPAACCGVYGFKATRGRVPTGPTKGEIWGGMGTDGVLSRT
                     VRDTAAAMDAICGREAGAPYDSADPAQPFLASVGEARRKPLRIGVWTTAWDGIPIAPE
                     CLEGVRFTAQLCRDLGHEVVEAAPPALDYWRVVNSHINVLASNIVSSVHAKLAALKRA
                     LRDDDLEPALMDGYEMGKTLTAEQYIDAINCFHQAGRALQLDIDQYDLILSPMLAQLP
                     VPLGYLAMQGKFTDFREKIARYAVFSAVMNVSGQPAASMPVHWTQDGVPVGVQVMGQF
                     GRDDLVLRLSAEIERAAPWVDRFPARFRK"
     gene            complement(3268..4071)
                     /locus_tag="VGC69_19565"
     CDS             complement(3268..4071)
                     /locus_tag="VGC69_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY0297534.1"
                     /translation="MSIKHRFPPIDERLLLQSVSRALDVLEAFAEKPDMKSLSEIAAA
                     AGINKSAAQRISQTLLNRGYLEQTERGSLTLGRLLLDRAFDYLRSNPLIEKAVPILND
                     LRKTTGERVDLSLFDAEHDNLTVVYAVRTQSKRELFYATLAGRRMPTCVATGGRACMA
                     VLPDEVVEQIIADSELTMITPKTRIHPEQIWEKVRDARRDGYSVQEEETLLGEVALAA
                     VIRDHNGTPVGAIHIAGSLGEWASDEFCKRFSPLALEAARALSLWNSHR"
     gene            complement(4111..4434)
                     /locus_tag="VGC69_19570"
     CDS             complement(4111..4434)
                     /locus_tag="VGC69_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0297535.1"
                     /translation="MPTITFIAADASQTEVQAANGDSVMHAALGAHLKGILGECGGSL
                     ACATCHVYVDEAWADKLAPMSDLENEMLDATACERKPGSRLCCQIEMSDALDGLKIAL
                     PESQL"
     gene            complement(4459..5676)
                     /locus_tag="VGC69_19575"
     CDS             complement(4459..5676)
                     /locus_tag="VGC69_19575"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0297536.1"
                     /translation="MSDITLEAPGKAPELDVDPYDEAVLDSPYAVHAAIRAAGPVAYL
                     KRYGVYAMGRHRNVQPTLKDWQTFSSTGGSGIADIRKPGNWRPASAIVEVDPPRHTYV
                     RSAMQKVMSPQLIRSWREMFTREAERLVDELVRKEYVDGVHDLSEAYIATTFPPALGL
                     RDSPERRENLFLLGSLNFDAQGPRNPRYEATQQRADEIMPWHDAMMRRENLLPGGFGA
                     RLYDAVDAGELEPELAPLLVRSFLRGGLDTTSSSISAALWYLAMNPAQYALLHEDPSR
                     ARPALEEAMRLETPIQTAARLTTREVVVDGTAIPNDSKVMMMLASANRDPDFWDRPDE
                     YDLTRSTLGHVALGNGIHMCIGQMIARLEGECILAAVAQRVRGIGLEGAPVRRINNVL
                     RSLKSLPLRLEAA"
     gene            complement(5686..6516)
                     /locus_tag="VGC69_19580"
     CDS             complement(5686..6516)
                     /locus_tag="VGC69_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY0297537.1"
                     /translation="MKHDKSVKWPGGARCAIMMTFDFDAETLWTSRDPANANRPGILS
                     QGTYGAKVGVPKLVEMLDEEDVKATFFVPGWTAENHTDKVELILKGGHEVGHHSYSHR
                     WSDGDERAEIEEMEKGLDALKRTVGVVPKGYRSPAGEVSPKLFELLKQHGFLYDTSLM
                     DDINPYRHKMPDGSQGVIELPWHWSLDDAPYALFSVKNPRPIFTNEHMLSIFKAEFRE
                     IYRWGGLFDLVCHPQISGRPSRIALLRELIAYIRTFPDVWFATGAEVAQSWSDAHENK
                     "
     gene            complement(6556..7305)
                     /locus_tag="VGC69_19585"
     CDS             complement(6556..7305)
                     /locus_tag="VGC69_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0297538.1"
                     /translation="MSKRFQGKSVIVTGAARGIGAATAKRFADEGASVLLADVLPEVL
                     ETAEAVGGQGVVVDVTASEAGALLARTALEAFGRIDVLVNNAGIGGSKRLEDSDDALL
                     ARIVDTNLGSMMRITRAVLPHLPRPGGSIVNLSSIFGLVGYPGTTAYGVAKAGVAQFT
                     QQLTSEVAHLGIRVNAIAPGVVVTPMTEGHLKNPHYVKLMVDSTPMGRVCQPEEQAAV
                     IAFLASDEASFVCGVTIPVDGGYLAARYIPN"
     gene            complement(7348..8556)
                     /locus_tag="VGC69_19590"
     CDS             complement(7348..8556)
                     /locus_tag="VGC69_19590"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0297539.1"
                     /translation="MTDAAIAAAAPELDIDPYEDAVLESPFAMFEAIRQAPAVYLKRH
                     DVYAIGRHREVQAAMKDWQTFSSTGGIGLTDVRKPDTWRARSPIGDVDPPDHTKVRTV
                     MQRILSPQVIRAWRAGFEEEAERLLDELVGKEYVDGVQDLAEAYVSTSFPKVMGIRHT
                     PQLRENWYLMGELNFDSQGPRNARYLATQQRADELISDWQQASMQRESLLPGGFGIKI
                     FEAADAGAMAPELAPLLIRSFLRGGLDTTTSMLSAVLWYLARDPSQYARLREDPALAR
                     PALDEAMRLETPSQTAYRLTMRDVVVDGTRIPADKKVMLQLGAANRDPAFWDRPDAFD
                     VQRNPVGHLALGHGIHMCIGQMVARLEGECVLNALTRRVSRLGLEGPPARKLNNALRG
                     FKTLPLRLQA"
     gene            complement(8603..9373)
                     /locus_tag="VGC69_19595"
     CDS             complement(8603..9373)
                     /locus_tag="VGC69_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0297540.1"
                     /translation="MSEQRFAGRVAFVTGAASGLGRASAVMLAREGARMMLFDRDAAG
                     LKDVQALCPGSIVHVGDASVAADVSAAAELSRKALGPAEFLVASAGINGIPKQAIEYT
                     EEEWDRLFAINVKGPWLACKAIIPQMRELGKGSIVLFSSTAGLAGSGFLPAYSASKGA
                     VALFARSLALNHAREGIRVNCVCPGTIDTPMAQQMFVQAAELGGAAPPTVDVMRNRIP
                     MGRFGLAEEIADAVLYFLSDAASYTTGVTMPIDGGLKA"
     gene            complement(9366..10085)
                     /locus_tag="VGC69_19600"
     CDS             complement(9366..10085)
                     /locus_tag="VGC69_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297541.1"
                     /translation="MLRLNNIVAGYGRTRILHGVSLEVPKGSLVALLGGNGTGKSTTL
                     KSIVGIVTPQEGDIVFDGQLIKDVPVEQRSALGLSLVPQGKEVFGAMSVEENLLMGAY
                     HRRRDRVGVAEDLEGIYLRFRRLRERRNVAAGMLSGGERQMLAIGRSLMSRPRMLLLD
                     EPSAALAPKVVEEIADAILALRELGLTLLLVEQNVGMALDIADYIYVIRGGRIAYERE
                     VGEGVAMDELREFYLGGKDHV"
     gene            complement(10072..10863)
                     /locus_tag="VGC69_19605"
     CDS             complement(10072..10863)
                     /locus_tag="VGC69_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0297542.1"
                     /translation="MSAAPILQARGIHRRFGGVVAVKDASLTVHAGEIVGLIGPNGSG
                     KSTLVNLITGELEVNGGTFTFCGKDVTALPPYKRARLGMARSFQMIRLFGSLSVEENL
                     LLAHHIHMKSSTLDTIVGSARAVQEEDEVRAKARELLQWFELETFAKRPAMALSIGQQ
                     RMVELARGLISQPRLFLLDEPAAGLSPPNVDRLIAIIGRMRAEFGMSILLVEHDMRVV
                     RELCDQVVVLDSGEMIAQGKPEVVVNDPVVVEAYIGSGWKRRAKA"
     gene            complement(10860..11816)
                     /locus_tag="VGC69_19610"
     CDS             complement(10860..11816)
                     /locus_tag="VGC69_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0297543.1"
                     /translation="MKKNLSLAILVVLLALAPVLATGSSYIVSLLLLMLVYVILTTGL
                     NLVVGYCGQFSFAQGAFFGIGAYTAGIMSRDLHTGFWVHLPAGMILAGLFGLLLGIPA
                     LRLKGHFLAIVTIAFQTIVYLGLVQWTSFTGGQNGLMVPPVGKVALFGATLFTIEGLN
                     SNYWMVLVATVVLVFVAARLVKSRLGREWVAVRDDEVLASAIGLNATRAKLTAFVASA
                     AFAGAAGVMMAHYMGNITPDDYTIWISCTVVAMMIVGGRGTFYGPMLGVALLTLLPEM
                     LGDFARYRMLFFGVLLVLSIALLPEGIAGRMRKLREMKEAQP"
     gene            complement(11813..>12579)
                     /locus_tag="VGC69_19615"
     CDS             complement(11813..>12579)
                     /locus_tag="VGC69_19615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0297544.1"
                     /translation="FAHGEFYMLGAYTGVILVTTYGWPVWAAMGGVLLVAIILGVGAE
                     GLLHKMLKDRDATSSIISSFGLAVVLQNSALIGFGPSPMMLQTRYSTIGVDMGPVYLP
                     LQQLLIPVAMALLIALLYLVLRYTWTGRSLRAMAQHATVARLCGVQVRRVAIIAFIVA
                     ALLAGIAGFLMSSVYMVYPIIGNMIVLKAFTVVILGGMGNVAGAAAAGLLLGIVESLT
                     SAYLGNGLRDIVGFVIVIAVLLLRPQGLFGKSFQRS"
BASE COUNT         2053 a         4301 c         4161 g         2064 t
ORIGIN      
        1 aatcagcaga atgttcggcg cgcggcgcag cagccacgcc agcgcgagct gcatgggcgt
       61 cgcgcccacg gacgccgcga tccggttcaa ttcgcccgac tggatgggcg tgaacccgcc
      121 cagcgggaaa aacggcacgt aggcaatgcc ctgctcggcg agcgcatcga tcagcgcgtc
      181 atcgtcgcga tgcacgaggt tgtagtgatt ctgcacgcag gcgatttcgg cgatgccctg
      241 cgcctcctcg acctgcgcgg cgctcgcatt gctcaggccg atgtggcgca ccaggccgcg
      301 ctgctgcagt gccgccagcg cctcgacctg ccgcgcgatc gagccctcgg acggcacatg
      361 gatattgccc atgatgcgca tgttcacgac atcgagcgca tccagcccca ggttgcgcag
      421 attgtcgtgc accccgcgtt cgatgtccgc gggttcctgg gccgcaagcc acgcggcatc
      481 gtcgccgcgc acggccccga ccttggtgac gatgagcaga tcgccgggat acgggtgcag
      541 cgcctccttg atgaggcggt tggtgatgtg cggcccgtag aagtcgctgg tatcgatgtg
      601 attcacgccg gcggccagcg cctcgcgcag cacggcgacg gccgcggcgg gatccttggg
      661 cggcccgaag acgcccgggc cggcgagctg catggcgccg tagcccatgc gattgacctg
      721 gcggccggcg aacggatagg tgccggcgaa agtggaattg gacatgtcgc gctcctgatt
      781 gatgacagcg accagtatgg ccagaacctc tttgtttgat aatctaccca aatccaaaca
      841 ggtcgttcga aatttcgaac aatattccca tggacatgag tgatctcgcc gccttcgtct
      901 cggtcgccca ggccggcggt tttcgcgatg cggcgcgcgt gggcggcgtc tcggcctcga
      961 gcctgagcac ggccgtgcag cggctcgagg ccaagctcgg gatgcgcctg ctcaaccgca
     1021 cgacgcgcag cgtcgccccg accgaggccg gccggcgcct gctggagcag cttgcgccgc
     1081 tctttggcga aatcgaagcg gcgctcgacg tgctgaacgg ttttcgcgac aggccggtcg
     1141 gcacgctgcg gctcaatgtc ccggccggcg tctcccacac cctcctgccc cccatcctgt
     1201 ccgggttcct gaaggcctat cccgagatcc aggtcgaggt cgtcgtcgag gacggcttcg
     1261 tcaacctgct cgaaatcggc tgcgatgcgg gcatccgcta cgacgagcgc ctcgagcagg
     1321 acatgatcgc cgtgccgatc gggccgcgcg tccagcgttt cgcgacagcc gccgcgcccg
     1381 cctatctcga cgcgcgcgga cgccccggcc atcctcgcga cctgctaggg catgcctgcc
     1441 tgcgcggcca gttcgcgggc ggcgtgacgc cgctctggca tttcgagcgc gacggggaag
     1501 tgatgcacat cgatccgccg cccgggccct tgctggtgcg ccccggcgtc gcgatcacgc
     1561 tcatggtccg cgccgcgatc gacgggctgg gcatcatcca tctttgggaa gacatgctgc
     1621 gcccgcagct ggacagcggc ctgctggcgc cgctgctcga accctggtgg caacgcttcc
     1681 cggggccctt tctctactac cccgggcgca ggcacctgcc cgcgccgcta cgggcatttg
     1741 tcgacttcct gaagaccccg gcgccggcgc cgcgcgcggc gcctgacggc tgagggcgct
     1801 tatttcctga atcgagccgg aaagcgatcc acccagggcg cggcgcgctc gatctcggcc
     1861 gacaggcgca gcaccaggtc gtcgcggccg aactggccca tgacctgcac gccgaccggc
     1921 acgccatcct gggtccagtg caccggcatg ctggcggccg gctggccgga cacgttcatc
     1981 accgccgaaa acaccgcgta gcgggcgatt ttctcgcgga agtcggtgaa cttgccctgc
     2041 atggccaggt agcccagcgg caccggcagc tgcgccagca tgggcgaaag aatcaggtcg
     2101 tactggtcga tgtcgagctg cagcgcgcgg cccgcctggt gaaagcaatt gatcgcatcg
     2161 atgtactgct cggccgtgag cgtcttgccc atttcgtagc cgtccatcag cgccggctcc
     2221 aggtcgtcat cgcgcagggc gcgcttgagc gcggccagct tggcatgcac cgaggacacg
     2281 atgttcgagg ccagcacatt gatgtggctg ttcacgacgc gccagtagtc cagcgccggc
     2341 ggcgccgcct cgaccacctc gtggcccagg tcgcggcaca gctgggccgt gaaccgcacg
     2401 ccttccaggc attccggcgc aatgggaatg ccgtcccagg ccgtcgtcca gacgccgatg
     2461 cgcagcggct tgcgcctggc ctcgccgacc gacgccagga aaggctgggc cggatcggcg
     2521 ctgtcgtagg gcgcgccggc ttcccggccg cagatcgcat ccatggccgc cgccgtgtcg
     2581 cgcaccgtgc gcgacagcac gccgtcggtg cccatgccgc cccagatctc gcccttggtg
     2641 gggccggtgg gcacgcgccc gcgcgtggcc ttgaagccat acaccccgca gcacgcggcc
     2701 gggatgcgga tcgagccgcc gccgtcgctg ccatgggcca ccggcacgat gcgcgaggcc
     2761 acggccacgg ccgccccgcc gctggagccg cctgtcgaac gcgcggcgtc ccaggggttg
     2821 atcgtgaccg cgccattggc caccgcctcg gtggtgggcg ccatgcagaa ctccggcacc
     2881 gaggtccgcg cgaaggggat caggccggcg gcctcgaagc gctgcgtcag cgtggcatcg
     2941 aaggtaaagg aagtatcggt gaacaggcgc gaacccaggc tcatgggcag gcgcttggaa
     3001 gccaggctcg agtccttcag caggaaggga atgccctgga acgcgccggc gtgctgccat
     3061 tgcctggccc attgcagcgc gtcgtcataa cgctcgtagc ggatggcatt gagctcgacg
     3121 ccgcgcgtgc gcgacagggc gatggcgtgc tccatcagct gcgtcgacga gacctccttc
     3181 tttttcagca gcgccgccag cgccaggccg tccagttcct ggtattcggt ttcagtcatc
     3241 agtcttcctt tctaggtgat ctcgttatca gcgatgcgag ttccacaggc tcaaggcgcg
     3301 ggccgcttcg agcgccagcg ggctgaagcg cttgcagaat tcatcggagg cccattcgcc
     3361 caacgagccc gcgatgtgga tggcgcccac gggcgtgccg ttatggtcgc ggatgaccgc
     3421 cgccagggcc acttcgccca gcagcgtctc ttcttcctgg acggaatagc cgtcgcggcg
     3481 ggcatcgcgc actttctccc agatctgctc ggggtgaatc cgggtcttgg gcgtgatcat
     3541 cgtgagttcg gagtccgcga taatctgctc gacgacctca tcgggcagca cggccatgca
     3601 ggcccgcccg ccggtggcga cgcaggtcgg catgcgccgc cccgccagcg tggcgtagaa
     3661 gagctcgcgc ttgctctggg tccgcacggc atacaccacg gtcaggttgt cgtgctcggc
     3721 gtcgaacagg ctcaaatcca cgcgctcgcc cgtggtcttg cgcaggtcgt tgaggatggg
     3781 cacggctttt tcgatcaggg gattggagcg caggtaatcg aaggcccggt ccagcagcag
     3841 cctgcccagc gtgaggctgc cgcgctcggt ctgttccagg tagccccggt tgagcagggt
     3901 ctggctgatg cgctgcgcgg cgctcttgtt gatgcccgcg gccgccgcga tctcgctcag
     3961 cgacttcatg tcgggctttt ccgcgaaggc ctccaataca tccagtgccc gcgataccga
     4021 ctgcagcagc agacgttcgt caatgggagg aaagcggtgc ttgatgctca taaatgaata
     4081 cttctgctaa atgggctggc cgggcaggac tcacagctgc gattccggca aagcgatctt
     4141 caggccgtcg agcgcgtcgg acatctcgat ctggcagcac aggcggctgc cgggcttgcg
     4201 ctcgcatgcc gtggcgtcga gcatctcgtt ttccaggtcg gacatgggag ccagtttatc
     4261 cgcccaggcc tcgtccacat agacgtggca ggtcgcgcaa gccagggatc cgccgcactc
     4321 gcccaggatg cccttgaggt gggcgcccag cgcggcgtgc atgacgctgt cgccattggc
     4381 ggcctggact tccgtctgcg aggcgtcggc ggcgatgaaa gtgatggtcg gcatgaagct
     4441 tcctttatgc aggtcaggtc aggcggcctc gagccgcagc ggcagcgact tcaggctgcg
     4501 caacacattg ttgatacggc gcaccggcgc cccttccagg ccgatcccgc gcacgcgctg
     4561 cgccacggcc gccaggatgc attcgccttc caggcgcgcg atcatctggc cgatgcacat
     4621 atggatgccg ttgcccagcg ccacatggcc cagcgtggag cgggtcaggt cgtattcgtc
     4681 gggccggtcc cagaaatcgg ggtcgcgatt ggccgaggcc agcatcatca tgaccttgct
     4741 gtcattgggg atcgccgtgc cgtcgaccac cacctcgcgc gtggtcaggc gggcggccgt
     4801 ctggatgggg gtttccaggc gcatggcctc ttccagggca ggccgcgcgc gggacggatc
     4861 ctcatgcagc agcgcgtact gcgccgggtt catcgccagg taccacagcg ccgccgagat
     4921 cgagctgctg gtggtgtcca gcccaccgcg caggaacgaa cgcaccagca ggggcgccag
     4981 ttccggctcg agttcgcccg cgtcgacggc gtcgtacaag cgcgcgccga agccgccggg
     5041 caacaggttc tcgcgccgca tcatggcgtc atgccagggc atgatctcgt cggcgcgctg
     5101 ctgcgtggcc tcgtagcggg gattgcgcgg cccctgcgcg tcgaagttca gcgagcccag
     5161 caggaacagg ttctcgcggc gctcgggcga atcgcgcagg cccagggccg gcggaaaggt
     5221 cgtggcgata taggcttccg acaggtcgtg gacgccgtcc acatattcct tccgcaccag
     5281 ctcgtcgacc aggcgctcgg cctcccgggt gaacatctcg cgccaggagc ggatcagctg
     5341 cggcgacatg accttctgca tggccgagcg cacataggta tgccgcggcg ggtcgacttc
     5401 gacaatggcg ctggccggac gccagttgcc cggcttgcgg atgtcggcaa tgcccgaccc
     5461 gcccgtggac gagaaggtct gccagtcctt cagcgtgggc tgcacattgc gatggcgccc
     5521 catggcgtag acgccgtagc gcttgagata ggccaccggc ccggccgcgc ggatggcggc
     5581 gtggaccgcg tagggcgagt cgagcacggc ctcgtcatag gggtcgacat ccagctccgg
     5641 cgccttgccc ggcgcctcca gggtgatgtc actcatggca gcctctcact tgttctcgtg
     5701 ggcatccgac caggactggg ccacctcggc gccggtggcg aaccacacgt cggggaaggt
     5761 gcggatatag gcgatcagtt cgcgcagcag cgcgatgcgg ctggggcggc cgctgatctg
     5821 cggatggcac accaggtcga acaggccgcc ccagcggtag atctcgcgga actcggcctt
     5881 gaagatgctg agcatgtgct cgttggtgaa gatgggacgc gggttcttca ccgagaacag
     5941 cgcatagggc gcatcgtcca ggctccagtg ccagggcagc tcgatcacgc cctggctgcc
     6001 gtcgggcatc ttgtggcggt aggggttgat gtcgtccatc agggacgtgt cgtacaggaa
     6061 gccgtgctgc ttgagcagct cgaagagctt ggggctgacc tcgcccgccg gcgagcggta
     6121 gcccttgggc accacgccga cggtgcgttt gagcgcgtcc aggccttttt ccatttcctc
     6181 gatctcggcc cgctcgtcgc cgtccgacca gcggtgcgaa tagctgtggt gcccgacctc
     6241 gtggccgccc ttcaggatga gctcgacctt gtcggtatgg ttttccgccg tccagcccgg
     6301 cacgaagaac gtggccttga cgtcttcttc atccagcatt tcgacgagct tgggcacgcc
     6361 caccttggcc ccgtaggtgc cctggctgag gatgcccggc cggttggcgt tggccggatc
     6421 gcgcgacgtc cacagcgttt cggcatcgaa atcgaaggtc atcatgatcg cgcagcgcgc
     6481 gccgcccggc catttgacgc tcttgtcatg tttcatggtc ttcactcccg gcgctcagcg
     6541 cgcccaagtc atcgatcagt tggggatgta tcgggcggcc aggtagccgc catcgacggg
     6601 tatcgtcacg ccgcacacga acgacgcctc atcggacgcc aggaacgcga tcacggcggc
     6661 ctgctcctcg ggctggcaga cgcggcccat cggggtcgag tccaccatca gcttgacgta
     6721 atgcgggttt ttcaagtgtc cctcggtcat gggcgtgacc accacgcccg gggcgatggc
     6781 attgacccgg atgcccaggt gcgcgacctc gctggtgagc tgctgggtga actgcgccac
     6841 gccggccttg gccacgccat aggccgtggt gccgggatag cccaccaggc cgaagatcga
     6901 cgacaggttc acgatactgc cgcccggcct gggcaggtgc ggcagcaccg cgcgcgtgat
     6961 gcgcatcatc gaccccaggt tggtgtcgac gatgcgggcc agcagcgcat cgtccgagtc
     7021 ttccaggcgc ttggagcccc cgatgccggc attgttgacc agcacgtcga tgcggccgaa
     7081 ggcctcgagc gccgtccgcg ccagcaaggc gccggcttcg gaagccgtca cgtcgaccac
     7141 aacgccctgc ccgcccacgg cttcggccgt ctccagcacc tcgggcagga catcggccag
     7201 gagcaccgag gccccttcat cggcgaagcg cttggccgtg gccgcgccga tgccgcgcgc
     7261 cgcgcccgtg acaatgacgc tttttccctg aaatcgtttg gacatggtgt tcatcctgtt
     7321 cgtttgctgt tttccggcgc cgtccgctca ggcctgcagg cgcaggggca aggtcttgaa
     7381 gccgcgcagg gcattgttga gcttgcgcgc cggcggcccc tccaggccga gccggctcac
     7441 ccgcctggtc agcgcgttga gcacgcactc gccttccagg cgcgcgacca tctgtccgat
     7501 acacatgtgg atgccgtggc ccagcgccag gtgcccgacc gggttgcgct ggacgtcgaa
     7561 cgcgtcgggc ctgtcccaga aggccggatc gcggttggcc gcgcccagct gcagcatcac
     7621 tttcttgtct gcgggaatgc gcgtgccgtc caccacgaca tcgcgcatgg tcagacggta
     7681 ggccgtctgc gaaggcgtct ccagccgcat ggcctcgtcc agcgccgggc gggccagcgc
     7741 cggatcctcg cgcagccggg catattgcga cgggtcccgg gccaggtacc acagcaccgc
     7801 ggacagcatg ctcgtcgtcg tgtccaggcc gccgcgcagg aaggaacgta tcagcagggg
     7861 cgccagttcc ggcgccatcg cgccggcatc ggccgcctcg aagatcttga tgccgaaacc
     7921 gcccggcagc aggctctcgc gctgcatgct ggcctgttgc caatcactga tgagctcgtc
     7981 ggcgcgctgc tgcgtggcca ggtagcgggc gttgcgcggc ccctggctgt cgaaattgag
     8041 ctcgcccatc agataccagt tctcgcgcag ctggggcgta tgccgtatgc ccatgacctt
     8101 gggaaaggag gtcgacacat aggcttcggc aaggtcctgg acgccgtcca cgtattcctt
     8161 cccgaccagc tcgtcgagca gccgttcggc ttcttcttcg aagcccgcgc gccaagcgcg
     8221 tatgacctgc ggcgacagga tgcgctgcat gacggtgcgc accttggtat ggtccggcgg
     8281 gtccacgtcg ccgatggggc tcctggcgcg ccaggtatcc ggcttgcgca cgtccgtcag
     8341 gccgatgccg ccggtcgacg agaacgtttg ccagtctttc atcgccgcct ggacctcgcg
     8401 gtggcggccg atggcataga cgtcatggcg cttcaggtac accgccggcg cctggcggat
     8461 tgcctcgaac atggcgaagg gggactcgag caccgcgtcc tcgtaggggt cgatgtcgag
     8521 ttcgggcgcc gcggctgcaa tggctgcatc ggtcatggcc tggttccttt ccggcttgct
     8581 tgccgtgtcg cgcgcaggcc cgtcaggcct tgagcccgcc gtcgatgggc atcgtcacgc
     8641 ccgtggtgta gctggcggca tcgctcagga agtacagcac cgcgtcggcg atctcctcgg
     8701 ccaggccgaa acgccccatc ggaatgcggt tgcgcatcac gtcgaccgtc ggcggcgccg
     8761 ctccgcccaa ctcggcggcc tgcacgaaca tctgctgcgc catgggcgtg tcgatggtgc
     8821 ccggacacac gcaattgacc cggatgccct cgcgcgcatg gttcagcgcc aggctgcgcg
     8881 cgaacagggc cacggcgccc ttggaggccg aataggccgg caggaagccc gagcccgcca
     8941 ggcccgccgt cgacgagaac agcacgatcg agcctttgcc cagttcgcgc atctgcggaa
     9001 tgatcgcctt gcacgccagc cagggaccct tgacgttgat ggcgaagagc cggtcccact
     9061 cctcttcggt gtactcgatg gcctgcttgg gaatgccgtt gatgccggcc gaggccacga
     9121 ggaactcggc cgggcccaag gccttgcggg acagctcggc cgcggcgctc acgtcggccg
     9181 ccaccgaggc atcgccgaca tggacgatcg agcccgggca cagggcctgc acgtccttca
     9241 ggccggccgc gtcgcggtcg aacagcatca tcctggcgcc ttcccgcgcc agcatcacgg
     9301 cgctcgcccg gcccaggccg gaagccgcgc ccgtcacaaa agccacccgt cccgcgaaac
     9361 gttgctcaga catgatcctt accccctaga tagaattcgc gcagttcatc catggccacc
     9421 ccctcgccca cctcgcgctc gtaggcgatg cgcccgccgc ggatcacata gatgtagtcc
     9481 gcaatgtcca atgccatgcc cacgttctgc tccaccagca gcagggtcag gcccagttcg
     9541 cgcaaggcca ggatggcgtc ggcgatttct tccacgacct tgggcgccag cgcggccgac
     9601 ggctcgtcga gcagcagcat gcggggccgg gacatcagcg aacggccgat ggccagcatc
     9661 tggcgttcgc cgcccgacaa catgcccgcc gccacattgc gccgctcgcg caggcgccgg
     9721 aagcgcaggt agatgccctc caggtcctcg gccacgccga cccggtcgcg gcgccgatgg
     9781 taggcgccca tgagcaggtt ctcctcgacg ctcatggcgc cgaagacttc cttgccctgc
     9841 ggcaccagcg acaggcccag ggccgaccgc tgctcgaccg ggacgtcctt gatgagctgg
     9901 ccgtcgaaca cgatgtcgcc ttcctggggc gtgacgatgc cgacgatgct cttcaaggta
     9961 gtggacttgc ccgtgccgtt gccgcccagc agcgccacca ggctgccctt gggcacctcg
    10021 agcgacacgc catggagaat gcgcgtgcgg ccgtagcccg cgacgatgtt gttaagcctt
    10081 agcacggcgc ttccatcccg aaccgatata agcctcgacc acgaccgggt cgttgaccac
    10141 gacctccggc ttgccctggg cgatcatttc accgctatcg agcaccacca cctggtcgca
    10201 caattcgcgc accacgcgca tatcgtgctc gaccagcagg atgctcatgc cgaactcggc
    10261 gcgcatccgg ccgatgatgg cgatcaggcg gtcgacattg gggggagaca ggcccgccgc
    10321 cggctcgtcc agcagaaaca gcctgggctg gctgatcagg ccgcgggcca gttccaccat
    10381 gcgctgctgg ccgatggaca aggccatggc cgggcgcttg gcgaacgtct ccagttcgaa
    10441 ccattgcagc agctcgcgcg ccttggcgcg cacctcgtcc tcttcctgca cggcgcgggc
    10501 gctgcccacg atggtgtcca gcgtgctgga cttcatgtgg atgtgatgcg ccagcagcag
    10561 gttctcctcg acgctcagcg aaccgaacag ccgtatcatc tggaagctgc gcgccatgcc
    10621 caggcgggcc cgcttgtacg gcggcagggc cgtgacgtcc ttgccgcaga aggtgaacgt
    10681 cccgccattg acctcgagct cgcccgtgat caggttgacc agggtggact tgcccgagcc
    10741 attggggccg atcaggccga cgatctcgcc ggcatggacc gtcaggctgg cgtccttgac
    10801 cgccaccacg ccgccgaagc ggcggtggat gccccgggcc tgcaaaatgg gcgccgcgct
    10861 catggctggg cctccttcat ctcgcgcagc ttgcgcatgc ggccggcgat gccttcgggc
    10921 agcagggcaa tcgacagcac caggagaacg ccgaagaaca gcatgcggta gcgcgcgaaa
    10981 tcgcccagca tctcgggcag cagcgtcagg agcgccacgc ccagcatggg gccgtagaag
    11041 gtgccgcggc cgccgacgat catcatggcc accaccgtgc acgaaatcca gatggtgtag
    11101 tcgtcggggg tgatgttgcc catgtagtgc gccatcatca cgcccgccgc ccccgcgaat
    11161 gccgccgacg cgacgaatgc cgtcagcttg gcgcgcgtgg cgttcaggcc gatggccgaa
    11221 gccaggacct cgtcgtcgcg cacggccacc cattcgcggc ccaggcgcga tttcaccagc
    11281 cgcgcggcga cgaataccag caccaccgtc gccaccagga ccatccagta attggagttc
    11341 agcccctcga tggtgaacag ggtcgcgccg aagagcgcga ccttgccgac gggcggcacc
    11401 atcaggccgt tctggccgcc ggtgaacgag gtccattgca ccaggcccag gtacacgatg
    11461 gtctggaagg cgatggtcac gatggccagg aaatgccctt tcaggcgcag cgccgggatg
    11521 ccgagcagca ggccgaagag gccggccagg atcatgccgg ccggcaggtg cacccagaag
    11581 cccgtatgca ggtcgcggga catgatgccg gcggtgtagg cgccgatgcc gaaaaaggcg
    11641 ccctgggcga acgaaaactg cccgcaatag cccaccacca gattcaagcc ggtcgtcagg
    11701 atcacataca ccagcatcaa gagcagcagc gacacgatat agctgctacc cgtcgcgagg
    11761 acaggcgcca gggccagcag cacgaccagg atcgcgaggg agaggttttt tttcatgagc
    11821 gttggaacga cttgccgaac agaccctgcg gacgcagcag caggacagcg atgacgatca
    11881 cgaaaccgac gatgtcgcgc aggccgttgc ccaggtaggc cgaggtcagc gactcgacga
    11941 tgcccagcag cagcccggcc gccgcggcgc cggccacatt gcccatgccg cccaggatca
    12001 cgaccgtgaa tgccttgagc acgatcatgt tgccgatgat gggatagacc atgtacaccg
    12061 acgacatcaa aaagcccgcg atgccggcca gcaaggccgc cacgatgaag gcgatgatgg
    12121 ccaccctgcg cacctgcacc ccgcacagcc gcgccacggt ggcgtgctgg gccatggcgc
    12181 gcaggctgcg gcccgtccag gtgtagcgca gcaccaggta cagcagggcg atcagcagcg
    12241 ccatcgccac cgggatcagc aattgctgca ggggcaggta gaccggcccc atgtcgaccc
    12301 cgatggtcga gtacctggtc tgcagcatca tggggctggg gccgaaaccg atcagcgccg
    12361 agttctgcag caccaccgcc aggccgaagg aagagatgat ggaactggtc gcgtcgcggt
    12421 ccttgagcat cttgtgcagc aggccttccg cgcccacgcc caggatgatc gccaccagca
    12481 gcacgccgcc catggccgcc cagaccggcc agccgtaggt ggtgacgagg atcacccccg
    12541 tatacgcgcc cagcatgtag aactcgccgt gcgcgaaat
//
LOCUS       DASWPO010000365         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_266256, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000365 DASWPO010000000
VERSION     DASWPO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266256"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGC69_19620"
     CDS             complement(<1..269)
                     /locus_tag="VGC69_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0297545.1"
                     /translation="MDLRFTPEELAFREEVRTFIRENLPADTRDRMKLGHPPRKEDTV
                     RWQRILNAKGWAALSWPKEWGGPGWSAIQRMIFLEENLMAPAPELS"
     gene            complement(608..1264)
                     /locus_tag="VGC69_19625"
     CDS             complement(608..1264)
                     /locus_tag="VGC69_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY0297546.1"
                     /translation="MAFLLGTAIGFERQWRQRSAGLRTNVLVAVGAAAFADLGLRLRG
                     VEGSTQIVAYVVSGIGFLGAGVILKDGTNIRGLNTAATLWCSAAVGAFCGTGLTVEAV
                     LLTLTILAANTMLRPLVNWVNRRPITADITEALYQVHAICRPDAVADARDLLDAELAQ
                     ANYPIREIETLSSGDEWVELAATLVPTTADAAELDAVITALERSPQIRSATWTVATTA
                     "
BASE COUNT          256 a          568 c          531 g          287 t
ORIGIN      
        1 gacagctccg gtgccggcgc catcaggttt tcctcgagga agatcatccg ctggatggca
       61 ctccagcccg ggccgcccca ttccttcggc caggacagcg ccgcccagcc ctttgcgttg
      121 aggattcgct gccagcgcac ggtgtcttcc ttacgcggcg gatgccccag cttcatgcgg
      181 tcgcgcgtat ccgcgggcag attttcgcga atgaacgtgc gcacttcctc gcggaaagcg
      241 agctcttccg gggtgaagcg aagatccatg gactactcct cctactcgcc ggtaactcta
      301 atcgtgccgg gaattgtgcg caatccgccg cgcacgcggc cttccgcctg ccgctgaacg
      361 ctacagccgt cccgcgcgac gccgccgcgt cgcccgtacg cctgccacgt gccatgcccg
      421 ccgcacgtac gtctgccacg tgccgtgccc gccacaggcc gtgcccgccg cacgacgccc
      481 gccacaggcc gtgcccgtca catgccgtgt ccgccgcacg tacgcctgcc acgtgccaag
      541 cccgcggcac gctaagcccg tcacgggcca tgatcgtcgc ccgtgcatgc cgatccgtcg
      601 gcgcggccta ggctgtggtc gccactgtcc aggtcgcgga gcggatctgc ggcgagcgct
      661 ccagcgccgt gatgaccgcg tcaagttccg ccgcgtccgc cgtcgttggg accagggtgg
      721 cggcgagttc gacccattcg tcgccgctgc tgagggtctc gatctcgcga atcggatagt
      781 ttgcctgagc cagctcggca tccagcagat cgcgcgcgtc ggctaccgcg tcggggcgac
      841 agatggcgtg cacctgatag agcgcctcgg tgatgtccgc ggtgattggc cggcggttca
      901 cccagttcac gagtggccgg agcatggtgt tcgccgcgag aatggtgagc gtcagcagca
      961 ccgcctcgac ggtcagcccg gtgccgcaaa aggcgcccac ggcagccgaa caccacaacg
     1021 tggcggcggt gtttaggccg cggatgttgg tcccgtcctt gaggatgacg ccggcgccca
     1081 gaaagcctat gccgctgacc acgtaagcga cgatttgtgt gctcccctcg acgccgcgca
     1141 gccgcaggcc aaggtcggcg aatgcggccg cacccacagc gacgaggacg ttggtgcgca
     1201 ggcccgctga gcgttgccgc cactggcgct cgaagccgat agccgtgccg agcaggaagg
     1261 ccatcacgag ggcgatgacc gtgcggagca ggaccgctag tcccagccag tcgccgaaaa
     1321 taagacgaag gtccaccacg ctgctcgctt ccggctgcac cggtcgccta ctgctgcgat
     1381 ccagcgatcc taggggctaa tttgtcgtct ggggcaacat ttcggtcgtc gtggatttgc
     1441 tacccggtgg gccggcagcg caattcggcc gacgcggaaa tttgtctttt atagcgccgg
     1501 tcggctttgt ccattcacca tgactggtcc atccctgcgc gagacggtgg cgtcgcacgt
     1561 gggcggcgcg tttcccggat gtcagtaggc tcggttacgg cagagtgcgc ccagggtctt
     1621 gcttcgcgcc agatagaatc cg
//
LOCUS       DASWPO010000366         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_266429, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000366 DASWPO010000000
VERSION     DASWPO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266429"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VGC69_19630"
     CDS             complement(<1..554)
                     /locus_tag="VGC69_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0297547.1"
                     /translation="MLSSLLAAFYETARQGGVTAAARQLSVSQPTVTTRIQQLEARYG
                     VTLFHRRGSRLELTQTGAGLMPLVRQMLQIQGDIDFKLRNAHELQGGSLHIGTTGPHY
                     IVSTIAAFRRQYPNAHIGIEVGNSLAVLESLFEHRVDIAVASQPLADSRVLQYEIASD
                     PLVLVAHRDHPLARRSTVTFQDMS"
     gene            874..2373
                     /locus_tag="VGC69_19635"
     CDS             874..2373
                     /locus_tag="VGC69_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY0297548.1"
                     /translation="MPSSDLSRRPYIVAAIMIAMAMVAIEATIVSTAMPQIAADLHGL
                     DLYSWVFSAFLLAQTTMTVVFGKLADVIGRRPMMLLGIAIFLLGSLLSGFAPSMPWLI
                     AFRLIQGVGAAAVQPLCMIIVADYYPAAQRGKVQGYLASVWAISAVLGPLVGGLIIHH
                     LSWAWIFWINVPVGIAAATTFMLYMRSDTVHKRPSIDVLGALLFTVAVASLMMVLADG
                     ANLPPGWVWKLLVVCVASAAIFAWHEQRVADPMVSFKLWGSRAMASANTATMLSGMAL
                     IGLTSFLPMYAQGVLHQSPIAAGIVLTLIMVGWPAGATLAARNFTKTGLRPVFIVGSV
                     LIPVGSLFMVFLQPGSSPLQAAAGSLIMGFGMGLFSISCLILIPEIVDRSQQGSATAS
                     NLFSRNLGSTLGAAVFGAVLNYGMTNNSTGTAITSDQLKHALESSDASGQVVGQILQH
                     SLHLTFLAVFIVSAAILALVLYIPRAATAHLSKDTLAEKAKHAAEHATG"
     gene            complement(2496..2825)
                     /locus_tag="VGC69_19640"
     CDS             complement(2496..2825)
                     /locus_tag="VGC69_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY0297549.1"
                     /translation="MSSESYYFWVVVLLIACAVITRAGYMLVGAYLPLPDGVRRALRY
                     APVAALTAIVVPDLLPWRDGAGPVFDTKLVAGVAGALIFLRTRNSVLVIVAGMVVLWL
                     LRWAAGA"
     gene            complement(2825..3571)
                     /locus_tag="VGC69_19645"
     CDS             complement(2825..3571)
                     /locus_tag="VGC69_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY0297550.1"
                     /translation="MAADSFDAAPDAAARREARAAFCEGARQISPALVVNSTWGLVTG
                     VAMVKSGMSTLVALVITVFVYSGTLQLTVLPLLAAGAPLWLVTAAGGVVNVRFVIFDA
                     GLYPFFRDRPWFERMALGFFNSDVAFAMFMARHADSPTPATPAQRWYYIGLILPCWIT
                     WELFSIAGVYLSAFVPTSWSLEFAATLALLAMAVPMASSRPMLATMAAAGVVAWLGQA
                     LPLRLGLAASVVLGIVAGMLAERRIHKKRA"
     gene            3672..>4006
                     /locus_tag="VGC69_19650"
     CDS             3672..>4006
                     /locus_tag="VGC69_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY0297551.1"
                     /translation="MSTMADRDGFIWYDGKLVPWREATTHVLTHSLHYGLSVFEGVRA
                     YKTDNGTAIFRLQDHTRRLLNSAHIYQMPVPFDAAALNQAQREVVKANKLESCYLRPL
                     VFYGSEKMGV"
BASE COUNT          695 a         1395 c         1276 g          640 t
ORIGIN      
        1 caggacatgt cctggaaggt gaccgtggac cggcgcgcca gggggtggtc gcgatgcgcg
       61 accagcacca gggggtcgga agcgatttcg tactgcagga cgcgcgaatc ggccagcggc
      121 tgcgacgcga cggcgatatc cacgcggtgc tcgaacaggg actcaagcac ggccagggaa
      181 ttgccaacct cgatgccgat gtgcgcgttc ggatactgcc ggcggaacgc ggcgatcgtg
      241 gacacgatgt agtgcggccc cgtcgtgccg atgtgcagcg agccaccctg cagctcgtgg
      301 gcatttcgca gcttgaaatc gatgtcgccc tggatctgca gcatctgcct gaccagcggc
      361 atgagtccgg cccctgtctg cgtcagctca agccggctgc cgcgccggtg gaacagggtc
      421 acgccgtagc gcgcctccag ttgctggatc ctcgtggtca cggtcggctg gctgaccgac
      481 aactgcctgg ccgccgccgt caccccaccc tgtcgcgcgg tttcgtagaa cgcagcaaga
      541 agggacgaga gcatggtgcg aaaggccgga ggatgcgcgg aaaggcgcag ggaaccggca
      601 gattctgcgg gcgccagttg acgccaagat gaataaaagt tgttttttgc cgacgaaata
      661 gcgctcgagg cccgccagcg cctgcgcata aaggcccttc cgggaacgat gcctcggcca
      721 aacaaaggca tctcgcctga taagcttgcc attatctttt atagttatac aattcgtgcc
      781 caattgtggg gcatgcgaca ttctccatcc ttcttctcgc ggccgcgcct cccggcgcac
      841 agcgccgcgt cgtcgtcgtt tctgctttct ttcatgccct cctccgatct atcccgccgg
      901 ccctatattg tggccgccat catgattgcc atggccatgg tggccatcga agcaaccatc
      961 gtttcaaccg ccatgcccca gatcgccgcc gacctgcacg gcctcgatct ctacagctgg
     1021 gtgttttccg ccttcctgct ggcccagacc accatgaccg tggtgttcgg caagctggcc
     1081 gacgtgatcg gccggcggcc catgatgctg ctgggcatcg ccatcttcct gctgggctcg
     1141 ctgctgtcgg gcttcgcccc gagcatgccc tggctgatcg ccttccgcct gatccagggc
     1201 gtgggggccg ccgccgtcca gccgctgtgc atgatcatcg tggccgacta ctacccggcc
     1261 gcccagcgcg gcaaggtgca gggctacctg gccagcgtct gggccatctc ggccgtgctc
     1321 ggccccctgg tgggcggcct gatcatccac cacctgtcct gggcctggat cttctggatc
     1381 aacgtgcccg tgggcattgc cgccgcgacc accttcatgc tctacatgcg cagcgacacg
     1441 gtgcacaagc ggccgtccat cgacgtgctc ggcgccctgc tcttcaccgt ggccgtggcc
     1501 tcgctgatga tggtgctggc cgacggcgcc aacctgcctc ccggctgggt atggaagctg
     1561 ctggtcgtgt gcgtggcgtc ggcggcgatc ttcgcctggc acgagcagcg cgtggccgac
     1621 cccatggtgt cgttcaagct gtggggcagc cgggccatgg cgtcggccaa cacggccacc
     1681 atgctgtcgg gcatggccct gatcggcctg acctccttcc tgcccatgta tgcccagggc
     1741 gtgctgcacc agagccccat cgcggcgggc atcgtgctga ccctgatcat ggtcggctgg
     1801 cctgccggcg ccacgctggc ggcccgcaac ttcaccaaga ccggcctgcg cccggtgttc
     1861 atcgtcggca gcgtcctcat cccggtcggt tcgctcttca tggtgttcct gcagccgggc
     1921 agctcgcccc tgcaggccgc cgccggctcg ctgatcatgg gtttcggcat gggcctgttc
     1981 agcatcagct gcctgatcct gatcccggaa atcgtcgacc gctcgcagca aggcagcgcc
     2041 acggcgtcca acctgttctc gcgcaacctg ggcagcaccc tgggcgccgc ggtgttcggc
     2101 gccgtgctga actacggcat gaccaacaac agcaccggca cggccatcac ctcggaccag
     2161 ctcaagcatg ccctggagtc cagcgatgcg agcggccagg tggtgggcca gatcctgcag
     2221 cactcgctgc acctgacctt ccttgccgtg ttcatcgtct cggccgccat cctggccctg
     2281 gtgctgtaca tcccgcgcgc cgccaccgcg cacctgtcga aagacacctt ggccgaaaaa
     2341 gccaagcacg ccgccgaaca cgcaacaggt taatcagggt tccagggcag gggcctgaaa
     2401 agcgtagcga gcggccggag ttcgcgccga gaagcgcagc cgtgctggtg cacggcgagc
     2461 atcgcaggtg cgaaatctgg ccgcgcagta gcttttcagg ctcctgcggc ccatctcaat
     2521 agccacaaaa ccaccatgcc ggcgacgatc accagcaccg aattgcgggt gcgcagaaag
     2581 atcagggcgc cggcgacgcc cgccaccagc ttggtgtcga acaccgggcc cgcgccgtcg
     2641 cgccagggca gcaggtcggg caccacgatg gccgtcagcg ccgccaccgg ggcatagcgc
     2701 agggcgcggc gcacgccgtc gggcaggggc aggtaggcgc ccacgagcat gtagccggcg
     2761 cgcgtgatca ccgcgcacgc gatcagcagc acgacgaccc agaagtaata ggactcgctc
     2821 gacatcacgc cctcttcttg tggatgcgcc gctcggccag catgcccgcc acgatgccca
     2881 gcaccacgga agccgccagt cccaggcgca agggcagcgc ctgccccagc caggccacca
     2941 cgccggccgc cgccatcgtg gccagcatgg ggcgggaact ggccatcggc accgccatgg
     3001 ccagcagggc cagggtggcg gcgaactcca gcgaccagga cgtcggcacg aacgcgctca
     3061 ggtagacgcc ggcaatggaa aacagctccc aggtgatcca gcagggcagg atcaggccga
     3121 tgtagtacca gcgctgcgcc ggcgtggccg gcgtgggcga atccgcgtgg cgcgccatga
     3181 acattgcgaa ggccacgtcg gaattgaaga agcccagggc catgcgctcg aaccagggcc
     3241 ggtcgcggaa gaacgggtac agccccgcgt cgaagatgac gaagcgcacg ttgaccacgc
     3301 cgcccgccgc ggtcaccagc cacagcggcg ccccggccgc caagagcggc agcaccgtca
     3361 gctgcagggt gccggagtag acgaacaccg tgatgacgag ggcgacgagc gtgctcatgc
     3421 ccgacttgac catcgccacc cccgtcacca gcccccaggt gctgttgacg acgagcgccg
     3481 gcgaaatctg ccgcgcgccc tcgcagaagg cggcgcgggc ttcgcgtcgg gcggcggcat
     3541 ccggtgccgc gtcgaaggag tcagcggcca cgggtctcct ccgggcctgc gcagcgacac
     3601 aacataatgg cgcgcattgt aaccgcccgg cttgatacaa taagccccaa aatccaaggg
     3661 aggacaccac catgtcgacg atggcggatc gggacggctt catctggtat gacggcaagc
     3721 tcgtgccgtg gcgcgaggcc accacgcatg tcctgaccca ctccctgcac tacggcctgt
     3781 cggtgttcga aggcgtgcgc gcctacaaga ccgacaacgg cacggccatc ttccgcctgc
     3841 aggaccacac caggcggctg ctcaactcgg cgcacatcta ccagatgccg gtgcccttcg
     3901 atgccgcggc gctcaaccag gcgcagcgcg aggtggtcaa ggccaacaag ctcgaatcct
     3961 gctacctgcg ccccctggtc ttctacggct cggaaaaaat gggcgt
//
LOCUS       DASWPO010000367         6141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_2499485, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000367 DASWPO010000000
VERSION     DASWPO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499485"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VGC69_19655"
     CDS             complement(<1..816)
                     /locus_tag="VGC69_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEY0297552.1"
                     /translation="MSMPMKWTVWLVSCVLASQAVAQSAPPAGMPAPASLLPGGDASA
                     SGTPAMPAPPPPPGSPGAPGAPAATSVAGASAMHYSLEQLGAQYAVNLRGVDGSNSLE
                     VGVRDDQVVTGARLNLRYAYSPALLPDLSHINVLVNDQVATSIALPKETGGTSLQRTI
                     ELPPYLFTSFSTLRLQLIGHYTMQCEDPLHSSLWANISNRSMLDVETEPLLLPNDLAS
                     LPVPFFDRHDRRRLDLPFVFASNPSESALESAGVVSSWFGALAGYRGARFPVTV"
     gene            complement(906..3281)
                     /locus_tag="VGC69_19660"
     CDS             complement(906..3281)
                     /locus_tag="VGC69_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEY0297553.1"
                     /translation="MNTRFAAPGLSARRARLRLPHALAVAAALLCASADMMAVQAQNA
                     PLAPAGAPQAPAAAPAMTVPTAPAASPLPADAYTYSLPLSMLDASDGLMLRGTGNING
                     VSFGLRQDERVVRAQFDLDYSYSPALLPAISHIKVLLNSEVVGAIGLPQPAPDVTQPR
                     MVDLPVGALSEYNRLNLELVGHTSLREDDAMSPNLWAQIGPRGAVLLTVVPVDLPNDL
                     ALLPVPFFDAHDVRAQDLPVVLPQAPDASRLESAGIVSSWFGSLAGFRGAHFTASLGA
                     IPAHGHAVVLAQPGEIPGLTMPEVDGPTLAIVPNPNDPHGKLLLVTGHNEAEIRQAAS
                     ALTLGTQTLAGSVARIGGLVPIQPRKPFDAPNWLRHDRPVEFGELMPANNFTVEGLRP
                     GPITLNLRLPPGLFNWHGDGIQVDLHYRYTPRPGSSGSMLAVLAGSQPLQSQPLQSFP
                     IPPSDSPAYRRALGLTPTGHVQLRVPTYLLPPLATLQFRYVYEYLKRFDGTDNVSSPV
                     MSAIDPTSTIDISQLPRYAAMPDLEAFGDAGFPFTRMADLSETAVILPDQPEAGDYSA
                     YLTMMGGMGESTGYPTLGVTVAGAAQAESLRDKDLLVLASGDNQPLINAWRSDMPRGF
                     FGEGVRHTGLWGWLWDRWDSVSEPSDRRPVAAAAKAGSYRSDAEGGMVAGLESPLASG
                     RSVVVVSGNSSTGLDAMVRTLQANRYLLSTKPESKVRGSLAVVQGGAVTSLLDEQRYY
                     IGRLPPWLAVEWFFANHMLLLLCAMLASALLIGLLGSLALRMRAQRRLGNA"
     gene            complement(3278..5512)
                     /gene="bcsA"
                     /locus_tag="VGC69_19665"
     CDS             complement(3278..5512)
                     /gene="bcsA"
                     /locus_tag="VGC69_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016759 - cellulose synthase activity
                     [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HEY0297554.1"
                     /translation="MTQPRTKSTKSRAPRNDAAASQRVDDILLLPLWTHRWIQLLLLL
                     VAVLLVIPAITVDLSLVEQGLLAVCCIIAAMLMNKVRGRLITVSIVVLSIATSLRYMY
                     WRVTETLHFESVIDGVFGYILLGAELYALAMLLLSYFQTVWPLRRKPVPLPADASAWP
                     TVDVYIPTFSEPLSVVRQTVLAAASLDWPADRLRVYLLDDGRRDEFREFCEQVGVNYL
                     TRPDNLHAKAGNLNAALRRTDGEFIAVFDCDHIVTRSFLQICMGWFLKDPKLGMLQTP
                     HVFFSPDPFERNLGTFKNVPNEGELFYGVVQDGNDLWNATFFCGSCAVMRREALEAVG
                     GVATESVTEDALTSLKMNRQGYDTAYLGVPQAAGLATESLSRHIGQRVRWARGMAEIF
                     RNNNPLMGTGLKLSQRFCYLAAMLHFFFGLPRIVFLLTPMAYLFFGARVFNASALMVM
                     TYALPHIVLSSLGGSRIQGSFRHSFWNEVYETVLAWYVTVPSIQALLWPGSKGFNVTQ
                     KGGLIKQAFFDWALARPYIGLLLVNVAGMLTGIVLLMFRPDFDIVMTILINMIWAFYN
                     VVITSAAVGVASEERQLRVTPRVQISLPAAIGLADGRRIACLTSDFSQGGLGLVLPQG
                     APDVPRGEKVTVSVFRDDYEGIFPAEVSLSRGSSLGVSLLALNIEQEKDLSRVTFGRA
                     DNWALSWGKKKPDAPLESMGQVILIGMGGFRLLLRNMFSWSVVRLNSPSQAKATRRQG
                     LKQP"
     gene            complement(5509..>6141)
                     /locus_tag="VGC69_19670"
     CDS             complement(5509..>6141)
                     /locus_tag="VGC69_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HEY0297555.1"
                     /translation="GGNPEDIAGLSRASVAGTPWRDACKRSASGIYVLPFGQVNEVDL
                     QALERQIAVRPDWLSSHLESLGLSNDALVILDTPPGPSEYLRQALGVADLVISVLLAD
                     AASYATLPMIEQMIQRYSGNRPGSLGHVCVINQVDAARRLSRDSVNLIRGALGDRVIG
                     VVHQDQSVSESLACGHTVVEHDATSLGRADLMQCADWVLRRLRAKEEVTQ"
BASE COUNT         1069 a         2065 c         2094 g          913 t
ORIGIN      
        1 caccgtcacc gggaagcgcg cgccgcggta gcccgccagc gcgccgaacc acgacgagac
       61 gacgccggcc gattccagcg cggattcgct ggggttggac gcgaacacga agggcaggtc
      121 caggcggcgg cggtcgtgcc ggtcgaaaaa cggcaccggc aagctggcca gatcgttggg
      181 cagcagcagc ggctcggtct cgacgtccag catgctgcgg ttgctgatgt tggcccacaa
      241 gctggaatgc aaggggtcct cgcactgcat cgtgtagtgg ccgatcagct gcaggcgcag
      301 cgtgctgaac gaggtgaaca gatagggcgg cagctcgatc gtgcgctgca ggctggtacc
      361 gcccgtttcc ttgggcaggg cgatggacgt tgccacctgg tcgttgacca gcacgttgat
      421 gtgcgacagg tcgggcagca gcgccggcga ataggcgtag cgcagattca ggcgcgcccc
      481 ggtgacgacc tgatcgtcgc gcacgccgac ttccagcgag ttgctgccgt ccacgccgcg
      541 caggttgacg gcatattgcg cgcccagttg ctccagcgaa tagtgcatgg ccgacgcgcc
      601 cgccacggaa gtcgccgcag gcgcccctgg cgctcccggc gaaccgggcg gcggaggcgg
      661 cgcgggcatg gccggcgtgc cggacgccga cgcatccccg ccgggcagca acgaagccgg
      721 cgccggcatg ccggcgggcg gcgcagactg ggccacagcc tgcgaggcca gcacgcagct
      781 gaccagccac accgtccatt tcatcggcat ggacatgcgg cgcgcagtgc tcggttcaga
      841 aatgtcgtgc cgtcttctac gcatggatcg tcgtcctttt cactacgttt atggctatca
      901 aaggctcagg cgttgcccaa tctgcgctgg gctcgcatac gcaaggccag gctgcccaac
      961 aggccgatca acaaggcgct ggccagcatc gcgcagagca gcagcagcat gtgattggca
     1021 aaaaaccatt ccacggccag ccagggtggc aggcggccga tgtaataacg ttgttcgtcg
     1081 agcaggctgg tgaccgcacc gccttgcacc acggccaggc tgccgcggac cttgctctcc
     1141 ggctttgtgc tgaggagata gcggttggcc tgcagcgtgc gcaccatggc gtccaggccg
     1201 gtggagctgt tgcccgacac gaccaccacg ctgcgccccg aggccagcgg cgactccagg
     1261 ccagccacca tgccgccctc ggcatcgcta cggtaggacc cggccttggc ggccgcggcc
     1321 accgggcgcc ggtcgctcgg ctccgagacc gaatcccacc tatcccacag ccagccccac
     1381 aggccggtgt gccgcacgcc ttcgccgaag aagccgcgcg gcatgtcgct gcgccaggca
     1441 ttgatcagcg gctggttgtc gcccgaggcc agtaccagca agtccttgtc gcgcagcgac
     1501 tcggcctgcg ccgcgccggc caccgtcacg cccagcgtgg ggtagccggt ggactcgccc
     1561 atgccgccca tcatcgtcag gtaggccgaa tagtcgcccg cttcgggctg gtcgggcagg
     1621 atcaccgccg tttcggacag gtcggccatg cgcgtgaagg ggaagcccgc atcgccgaaa
     1681 gcttccaggt cgggcatggc cgcgtatcgc ggcagctggg aaatgtcgat cgtcgaagtc
     1741 gggtcgatgg cgctcatgac cggactggat acgttgtccg tgccgtcgaa gcgcttgaga
     1801 tattcgtaca catagcggaa ctgcagcgtc gccagcggcg gcagcaggta ggtgggcacg
     1861 cgcagctgga cgtgccccgt cggggtcagg cccagcgccc tgcggtaggc gggcgaatcg
     1921 ctggggggaa tcggaaagct ttgcagcggc tggctctgca gcggctggct gcccgccagc
     1981 accgccagca tggacccgct cgagcccggt cgcggggtat agcggtagtg caggtccacc
     2041 tggatgccgt cgccgtgcca gttgaacagg cccggcggca ggcgcaggtt caaggtgatg
     2101 ggtccgggac gcaggccttc gacggtgaag ttgttggccg gcatcagctc gccaaactcc
     2161 accgggcggt cgtggcgcag ccaattgggc gcatcgaacg gcttgcgcgg ctggatcgga
     2221 accaggccgc cgatccgggc cacggagccg gccagcgtct gcgtgcccaa ggtcagcgcc
     2281 gaagcggcct ggcggatctc ggcctcgttg tggcccgtga ccagcagcag cttgccgtgc
     2341 gggtcgttgg ggttgggaac gatggccagg gtcgggccat ccacctcggg catggtcaaa
     2401 ccggggatct cgcccggctg cgccaacacc acggcgtggc catgcgcggg gatcgcgccc
     2461 aggctggcgg taaagtgcgc gccgcgaaag cccgccagcg agccgaacca cgacgacacg
     2521 atgcccgcgg attccagccg gctggcgtcg ggcgcctgcg gcaacaccac cggcaggtcc
     2581 tgcgcgcgga cgtcgtgcgc atcgaagaac ggcacgggca gcaacgccag gtcgttgggc
     2641 aggtccaccg gcaccaccgt cagcagcacc gcgccgcgcg gcccgatctg agcccacaga
     2701 ttgggactca tcgcgtcgtc ttcgcgcagg ctggtgtggc cgaccagctc caggttcagg
     2761 cggttgtact cggacagggc ccccacgggc aggtccacca tgcgcggctg ggtgacgtcc
     2821 ggcgccggct gaggcaggcc gatggcgccg accacctcgc tgttgagcag caccttgatg
     2881 tgcgaaatcg cgggcagcag cgccggcgaa taggaataat ccaggtcgaa ctgggcgcgc
     2941 accacgcgct cgtcctggcg caggccgaag ctcacgccgt tgatattgcc ggtgccgcgc
     3001 agcatcaggc cgtcgctcgc gtccagcatc gacagcggca ggctgtaggt gtaggcatcg
     3061 gccggcaggg gcgatgcggc cggcgcggtt gggacggtca tggccggcgc ggccgcaggc
     3121 gcttgcggcg ccccggccgg cgcaagcggc gcgttctgcg cctgcacggc catcatgtcg
     3181 gcggacgcgc acagcagcgc cgccgccacg gccagcgcat ggggcagtcg caagcgggca
     3241 cggcgcgcgc tcaagcccgg ggcagcgaac ctggtgttca tggttgcttc aacccctgcc
     3301 tgcgggtcgc cttggcctga gagggagagt tcagacgcac cacgctccac gaaaacatat
     3361 tgcgcagcag cagcctgaag ccgcccatgc cgatgagaat cacctgcccc atggactcca
     3421 gcggcgcatc gggcttcttc tttccccagg acagggccca gttgtcggcc cgcccgaaag
     3481 tgacccgcga caggtccttc tcttgttcga tgttcagggc cagcaggctc acgcccaggg
     3541 agctgccccg gctgagcgat acctcggcgg ggaaaatgcc ctcgtagtcg tcgcggaaca
     3601 ccgacacggt gaccttctcg ccgcgcggca cgtccggcgc gccttggggc agcaccagcc
     3661 ccaggccgcc ttgcgagaaa tcgctggtga gacaggcgat gcggcgaccg tcggccaggc
     3721 caatcgcggc gggcagactg atctgcacgc gcggcgtgac gcgcagttgc ctttcctcgc
     3781 tggcgacgcc gacggccgcg ctggtgatga cgacgttgta gaacgcccag atcatgttga
     3841 tcaggatggt catcacgatg tcgaagtcgg gccggaacat cagcagcacg atgccggtca
     3901 gcatccccgc cacattcacc agcagcaggc cgatataggg ccgggccagc gcccagtcga
     3961 agaaggcctg cttgatcagc ccgcctttct gggtcacgtt gaagcccttg ctgcccggcc
     4021 acagcagcgc ctggatcgac ggcaccgtga cgtaccaggc caggaccgtt tcgtagacct
     4081 cgttccagaa agaatggcgg aagctgccct ggatgcgcga gccgcccagg cttgacagca
     4141 cgatgtgcgg caaagcgtag gtcatgacca tcagcgccga ggcattgaac acgcgcgcgc
     4201 cgaaaaacag ataggccatg ggcgtgagca gaaacacgat gcgcggcaga ccgaaaaaga
     4261 agtgcagcat cgcggccaga tagcaaaacc gctgggacag cttcaggccc gtccccatca
     4321 aggggttgtt gttgcggaag atctccgcca tgccgcgcgc ccaacgcaca cgctggccga
     4381 tatggcgcga caggctctcg gtggccaggc cggccgcctg cggcacaccc agataggccg
     4441 tgtcgtagcc ctggcggttc atcttcagcg aggtcagggc gtcttccgtg acgctttcgg
     4501 tcgccacgcc gcccacggcc tccagagcct cgcgccgcat caccgcgcag gagccgcaga
     4561 agaaggtggc gttccacaga tcgttgccgt cctgcaccac gccatagaac agctcgccct
     4621 cgttgggcac gttcttgaaa gtgccgaggt tgcgctcgaa agggtcgggc gagaagaaca
     4681 cgtgcggcgt ctgcagcatg cccagcttcg ggtccttcag gaaccagccc atgcaaatct
     4741 gcaggaacga gcgcgtcacg atgtggtcgc agtcgaacac cgcgatgaac tcgccatcgg
     4801 ttctgcgcag cgccgcgttg aggttgcccg ccttggcgtg caggttatcg ggtcgcgtca
     4861 ggtagttcac gccgacctgt tcgcagaact cgcggaactc gtcgcgacgg ccgtcgtcca
     4921 gcaggtacac gcgcagccgg tcggccggcc agtcgagcga cgcggccgcc agcaccgtct
     4981 gccgcaccac gctcagcggc tcgctgaagg tggggatgta cacgtccacc gtgggccagg
     5041 ccgacgcatc cgcgggcagc ggcacgggct tgcgccgcag cggccagacc gtctggaagt
     5101 agctcaacag cagcatcgcc aaggcgtaca gttcggctcc cagcaggata tagccgaaca
     5161 cgccgtctat cacgctctcg aagtgcaagg tctccgtcac gcgccaatac atgtagcgca
     5221 gcgaggtggc gatcgacagc accacgatcg acaccgtgat cagccggccg cgcaccttgt
     5281 tcatcagcat cgcggcgata atgcagcaga ccgccaacag gccttgctcg accaggctca
     5341 agtccaccgt gatggcgggg atgaccagca gcacggcgac gagcagcagc agcagctgga
     5401 tccagcgatg cgtccacaag ggcagcagca ggatgtcgtc cacgcgctgg ctggcggccg
     5461 cgtcgttgcg aggggcgcgc gacttggtcg acttcgtccg cggctgcgtc attgggtcac
     5521 ctcttccttg gcgcgcaggc ggcgcagcac ccaatcggcg cattgcatca ggtcggcccg
     5581 gcccaggctg gtggcgtcat gctcgacgac cgtgtggccg caggccagcg attcgctcac
     5641 ggactggtcc tggtgcacca cgccgatgac gcgatcgccc agcgcgccgc ggatcaggtt
     5701 cacggaatcc cgggacagac gccgcgcggc gtcgacctgg ttgatcacgc agacatgccc
     5761 gaggctgccg ggccgattgc cgctatagcg ttggatcatc tgctcgatca tcggcagcgt
     5821 ggcgtacgag gccgcgtcgg ccagcagcac ggaaatcacc aggtcggcca cgcccagggc
     5881 ctggcgcagg tattcggacg gacccggcgg ggtatccagg atcaccagcg catcgttgga
     5941 caggcccagg ctctccaggt ggctcgacag ccagtccggg cgaacggcga tctggcgctc
     6001 cagcgcctgc aggtcgacct cgtttacctg gccgaaaggc agtacgtaaa tacccgacgc
     6061 gctgcgcttg caggcatcgc gccaaggcgt ccccgccacg ctggcgcgcg acaggccggc
     6121 aatgtcttcc gggttgccgc c
//
LOCUS       DASWPO010000368        15016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_803051, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000368 DASWPO010000000
VERSION     DASWPO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 15016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15016
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803051"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGC69_19675"
     CDS             <1..182
                     /locus_tag="VGC69_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0297556.1"
                     /translation="VLVRVGDDAARINLELLKRGIIVRPTAGDGLPEWLRISIGLPQE
                     NARFIEALTQILQRP"
     gene            179..1105
                     /locus_tag="VGC69_19680"
     CDS             179..1105
                     /locus_tag="VGC69_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY0297557.1"
                     /translation="MSSPGAAARIPVLAVVGVGLIGGSFAAALRQAGRAGRVLGVGRD
                     PQSLRRACELGLIDEAVGAAEAARRADLVMLAAPVGSLGPLLAEMRPHLKPGIVLTDA
                     GSTKAEVVRAARAALGDRIGCFVPGHPIAGAERTGPQAAEAGLYQGRIVVLCPLPENA
                     PQDVEQVRLAWQDCGAQVCDMEAGLHDRVLASVSHLPHFLAAVYMAQVAGEPDAAQRL
                     ALAGSGFRDFTRIAAGSPEMWRDIFLSNRAAMQAELAAVRAVLDQAEQALAAGDGAAL
                     LGLMEGASQARRNWHLGSGRLNNENTGSPKSP"
     gene            1102..2415
                     /gene="aroA"
                     /locus_tag="VGC69_19685"
     CDS             1102..2415
                     /gene="aroA"
                     /locus_tag="VGC69_19685"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY0297558.1"
                     /translation="MKLDHLDLPRATGARGTVALPGSKSISNRVLLLAALAAGVTEIG
                     GLLDSDDTRVMLAALRLLGVPVEDLGPGSVRVRGTAPFPVEQAEIFLGNAGTAFRPLT
                     AALALMGGDYRLSGVPRMHERPIGDLVEALNGLGARVGYLGQPGYPPLRIGRGRPRAD
                     APVRVQGSVSSQFLTALLLAAPLLARAAGRPVTLEVIGELISKPYIEITLNLMARYGV
                     AVAREGWQRFIVPADAAYLSPGRILVEGDASSASYFLALGAIGGGPVRVTGVGADSIQ
                     GDVAFARTLQAMGARVESGPDWLQASGVNAARGETLRAFDADFNLIPDAAMTAAALAL
                     YADGPCLLRNIGSWRVKETDRIHAMQTELAKLGAQVESGPDWLRVAPPAQWRDADIGT
                     YDDHRMAMCFSLAAFGPAKVRILEPGCVSKTFPTYFDIYADLIQT"
     gene            2513..3172
                     /gene="cmk"
                     /locus_tag="VGC69_19690"
     CDS             2513..3172
                     /gene="cmk"
                     /locus_tag="VGC69_19690"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY0297559.1"
                     /translation="MSHIVPVITIDGPTASGKGTVAHRVAQALGWAVLDSGALYRLTA
                     KAAIKHGIAAEDAPALARAAQSLDACFEGERILLEGEDVTDVIRQEQVGNLASRIAGY
                     PELRQALLARQRAFRVAPGLVADGRDMGTVVFPDAPLKVFLIAAAEARAQRRYKQLID
                     KGISANIPDLLRDLQARDARDMQRTAAPLAPAEDARELDSSNLTIEQTVQAVLSMWQA
                     R"
     gene            3332..5044
                     /gene="rpsA"
                     /locus_tag="VGC69_19695"
     CDS             3332..5044
                     /gene="rpsA"
                     /locus_tag="VGC69_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY0297560.1"
                     /translation="MSSLNQQVSGGESFADLFAQSLKSQDMKSGEVISAEVVRIDHNF
                     VVVNAGLKSEALIPLEEFLNDHGEVEVQPGDYVSVAIDSLENGYGDTILSRDRAKRLS
                     AWLSLETALENGELVTGTITGKVKGGLTVMTNGIRAFLPGSLVDLRPVKDTTPYEGKT
                     LEFKVIKLDRKRNNVVLSRRQVLEASMGEERQKLLETLHEGAVVKGVVKNITDYGAFV
                     DLGGIDGLLHITDMAWRRVRHPSEVLTVGQEVEAKVLKFDQEKSRVSLGVKQLGEDPW
                     VGLARRYPQGTRLFGKVTNLTDYGAFVEVEAGIEGLVHVSEMDWTNKNVDPRKVVTLG
                     EEVEVMVLEIDEDRRRISLGMKQCRQNPWEEFATNFKRGDKVRGAIKSITDFGVFVGL
                     PGGIDGLVHLSDLSWTDTGEEAVRNFKKGDELEAVVLGIDTDKERISLGVKQLEGDPF
                     NNFVATHDKGAVVPGTIKSVEPKGVVVTLSVDVEGYLRASEISSGRVEDATTVMKAGD
                     NIEAMIINVDRKTRSIQLSIKARDNAETAETIQRMSEASASSGTTNLGALLKAKLDQQ
                     RGEG"
     gene            5060..5416
                     /locus_tag="VGC69_19700"
     CDS             5060..5416
                     /locus_tag="VGC69_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY0297561.1"
                     /translation="MTKSELIAALAARYPQLAARDTDYAVKTMLDAMTQALASGQRIE
                     IRGFGSFSLSQRSPRVGRNPKSGEQVLVPGKQVPHFKAGKELRERVDLVAGELAGGAD
                     GSSDPTSSELDLHAMH"
     gene            5571..7130
                     /locus_tag="VGC69_19705"
     CDS             5571..7130
                     /locus_tag="VGC69_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914518.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY0297562.1"
                     /translation="MRHLEVIDYGVIAVYFALTAFIGFSATRRATSSDDLFLAGRSLG
                     PVVVGFSLFASNISSDTLVGLPGAAYRNGISASNYEWMAGVVLVFTAIFVLPVLMRSR
                     VATMPEFMERRFDWRLRRYLSVITLFLSIILDTAGTLYAGSLVLSIFIPGLALWQACW
                     VLALFTALYTACGGLRAVVYADILQALVLLGGSACLAIIMFGHFDYSWSVLTARVDAN
                     KLSLIRPLDDPGVPWLGLVTGLPIVGFYYWTMNQYVVQRILGARNAATAGRAALFAAL
                     LKLLPIFLMTIPGAMASVLLPGLAQPDQVYPAMVSAFAPTGIRGLILAGLMAALMSTV
                     SATLNSAATLVALDFVQPARPRLTSSQLAWVGRVATLFIALIAAAWAPMIQHFAGLWG
                     YLQQVFAFVASPLVATFLMGLWVARLGSSAALRGLVCGHVLSAVCFVLYQAGIVHLHF
                     TVLGGVLCAATALMTWAWMRVLRDRDQPDGQGREILSHARFNLAGMPRDVLWGCAAVL
                     VLTGVIVVALR"
     gene            complement(7178..8263)
                     /locus_tag="VGC69_19710"
     CDS             complement(7178..8263)
                     /locus_tag="VGC69_19710"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY0297563.1"
                     /translation="MKTHKIAVIAGDGIGKEVMPEGLRVVEAAAKRFGIKLAFKHIEW
                     ASCDYYAKHGDMMPPDWFDQLKDCEAIYFGAVGWPDIVPDHISLWGSLIKFRRDFDEY
                     VNLRPARLMPGVPCPLAGRKPGDIDMYIVRENTEGEYSSLGGKMFPGTDREFVIQESI
                     FSRKGTDRIIKYAFELAQSRPKKHLTSATKSNGIFISMPYWDERFAEMGKQYPDVRQD
                     KYHIDILTARFVISPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANINPDRTLPS
                     LFEPVHGSAPDIYGKNIANPVGMIWSGAMMLDFLGSGQGVYRQAHDAIMRAIEQTLAA
                     GQTTGDLGGKLDTTEAGMAVAAALEAA"
     gene            8392..8715
                     /locus_tag="VGC69_19715"
     CDS             8392..8715
                     /locus_tag="VGC69_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY0297564.1"
                     /translation="MRYLIWVLRLLVFIAVLIFAVKNTDPVKVNFYADYTVQSVPLIV
                     VMLVTFVVGTVFGLLLTVPVAMRRRREASRLRREVERLQAAAHPADVTPAMVPPESIA
                     PMSPL"
     gene            8746..10002
                     /gene="lapB"
                     /locus_tag="VGC69_19720"
     CDS             8746..10002
                     /gene="lapB"
                     /locus_tag="VGC69_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEY0297565.1"
                     /translation="MDFEPWWLIFIPMLFALGWLAARFDFRQMLRETRTLPDSYFRGL
                     NFLLNEEPDRAIDAFVEVAKLDPETTELHFALGSLFRRRGEMERAIRVHQSLLNRADL
                     PVAERDHAQHELAQDFLKSGMLDRAESTFSTLKDTRYALPALRSLVRIYESEHDWPRA
                     IEVVKTLQGLVDEPVPQIVHYHCELALTALSAKPVNFEAANVALDAADHAAKSFDAAI
                     SRGARVRIAMLRAQMAQLEQNPKRERLYLESILASAPEYAGLVAERLLANYTQTGEAA
                     DGLDVLSRHYAQHASLDLFNVVFRELRAQRGAAPAWRFAREALRSHPSLLGLDRLLEA
                     ELANPVPAAAIAGADESVPSDTRRGEAVSGGDLTLLRSLIHKHTQRLDRYVCRSCGFQ
                     PRSFYWQCPGCNSWETSSPRRLEELE"
     gene            9999..10943
                     /gene="rfaE1"
                     /locus_tag="VGC69_19725"
     CDS             9999..10943
                     /gene="rfaE1"
                     /locus_tag="VGC69_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY0297566.1"
                     /translation="MMADPSFPAQAISRSRVLVVGDVMLDRYWFGEVDRISPEAPVPV
                     VHVARREDRLGGAANVARNVAALGGLVTLVGVTGSDEAGARIGQLAQEAGVQSDLVSD
                     AEHPTTLKMRVLGRQQQLLRIDFEQHPSDGALDGVGAAVARHLPHHDVVVLSDYAKGA
                     LERVESLIALARQHKVPVLVDPKGDDYTRYKGATLVTPNRSEMQLAVGRWKSEDDLTA
                     RAQQLRADLQLEALLVTRSEQGMTLFADAGREHVDAQAHEVFDVSGAGDTVLATLALM
                     RAAGLSWAEAMGWANRAGGIVVGKLGTSTVTAAELAGV"
     gene            10946..11932
                     /gene="rfaD"
                     /locus_tag="VGC69_19730"
     CDS             10946..11932
                     /gene="rfaD"
                     /locus_tag="VGC69_19730"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY0297567.1"
                     /translation="MFIVTGAAGFIGSNLVRGLNRRGIDDIIAVDDLTDGDKFRNLAD
                     CRIADYLDKDDFRARVLEGRLPDVRAVLHQGACSDTTERNGRFMLDNNYRVTLELFEY
                     CQSRFIPFLYASSAAVYGGSSVYVEDPANEAPLNVYGYSKLLFDQVLRRRMAQRTSQV
                     VGLRYFNVYGPHEQHKGRMASVAFHNMNQFLAEGHVRLFGGWDGYGDGEQRRDFISVE
                     DVVAVNLYFLDHPEQSGIFNCGTGRAQPFNDVAAAVVNALRAERGEAALPLAELARAG
                     FVRYIPFPDDLKGRYQSYTQADVSNLRAAGFAAPMRDVQTGVAEYVRAWRAQ"
     gene            12064..12498
                     /locus_tag="VGC69_19735"
     CDS             12064..12498
                     /locus_tag="VGC69_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HEY0297568.1"
                     /translation="MNPFLHDPVARPAPRADVPTAVAASARRMRRRLMAGPMGRLAAA
                     GGLSLAMASAHALDVNAASAEQLEALRGVGPKTARIIVQERLRGGRFESIEDLSDRVR
                     GIGPKRAQALQASGLTVGAGAASAQAAGAAPRPAAPKSPGRP"
     gene            12630..13544
                     /gene="cysM"
                     /locus_tag="VGC69_19740"
     CDS             12630..13544
                     /gene="cysM"
                     /locus_tag="VGC69_19740"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEY0297569.1"
                     /translation="MNITDYPTIEQTIGNTPLVRLQRIPGQAGAARGNVILAKLEGNN
                     PAGSVKDRPAMSMIRRAEERGEIKPGDTLIEATSGNTGIGLAMAAAMRGYRMILIMPD
                     NLSLERRASMTAYGAQLILTPADKGGMEYARDLATEMQSEGKGKVLDQFANPDNPRAH
                     IETTGPEIWQQTGGRITHFVSAMGTTGTIMGVSTYLKGRSPAIQVIGAQPAEGSQIPG
                     IRKWPEAYLPKIFDAGRVDAYESIEQKDAEVMARRMAAEEGIFAGISSAGALIAALRV
                     AERASDATIVFVVCDRGDRYLSTGVFNP"
     gene            complement(13757..14929)
                     /gene="mltB"
                     /locus_tag="VGC69_19745"
     CDS             complement(13757..14929)
                     /gene="mltB"
                     /locus_tag="VGC69_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEY0297570.1"
                     /translation="MFICRRLLQIGVLTPFLLSACASPHPPATAPDLPSTASSSSPIR
                     IGPSEPTAPESSGEPSATVLPSGRLRPEVDAFARDLAARQNLPLDRVLGSLSSARYSA
                     TVARLILPSTPDHKVWRSWLTYRSRFVEPKRIRWGAEFWQANAATLNQAAQRYGVPAK
                     IIAGIIGVETIYGRNMGSFRVMDALATLAFDYPVPDKPDRAEMFRGQLADFITLALQG
                     RLDMDTRGSYAGAIGMPQFMPGSITRYAVDGSGNTGGPIDLANNPADAIMSVGNYLAS
                     HGWVRGVPVFAPVTLPADPSALVAGGLEPTLDWDKLKAAGARVSAGGGEGAWSRQPLG
                     IVDLVEEARGTAQYRTGTPNFFAITQYNHSYFYATAVSDLADAVAAQVAPASAGAL"
BASE COUNT         2334 a         5099 c         5062 g         2521 t
ORIGIN      
        1 tcgtgctggt gcgcgtgggc gatgatgctg cgcgcatcaa cctggaactg ctcaagcgcg
       61 gcatcatcgt gcgtcccacg gccggcgacg gcctgcccga gtggctgcgc atttccatcg
      121 gcttgccgca ggaaaatgcc cgcttcatcg aggcgctgac gcaaatcctg caaagaccat
      181 gagttccccc ggcgctgccg cgcgcattcc ggtgctggcc gtggtcggcg tcgggttgat
      241 cggcggctcg ttcgccgcgg ccttgcggca ggcgggcagg gcggggcggg tgctgggcgt
      301 gggccgcgac ccgcaatcgc tgcggcgcgc ctgcgaactg ggcctgatcg acgaggccgt
      361 cggcgccgcc gaggcggcgc gccgtgccga cctggtgatg ctggccgcgc cggtgggcag
      421 cctcggcccg ctgctggccg agatgcgtcc gcacctcaag cccggcattg tgctgaccga
      481 cgcgggcagc accaaggccg aggtggtgcg ggccgcgcgc gctgcgctgg gcgatcggat
      541 cggctgcttc gtgccgggcc atcccattgc cggcgccgag cgcaccgggc cgcaggccgc
      601 cgaggccggc ttgtaccagg gccgcatcgt ggtgttgtgc cccttgccgg aaaacgcgcc
      661 gcaggacgtc gaacaggtgc gcctggcctg gcaggactgc ggggcgcaag tatgcgatat
      721 ggaggccggt ctgcacgacc gcgtgctggc ctcggtcagc catctgccgc atttcctggc
      781 ggccgtctat atggcgcagg tggcgggcga gcccgatgcc gcgcagcgcc tggccctggc
      841 gggctcgggc tttcgggatt tcacgcgcat cgcggccggt tcgcccgaga tgtggcgcga
      901 cattttcctt tccaaccgcg cggccatgca ggccgagctg gccgctgtgc gcgccgtgct
      961 ggaccaggcc gagcaggcgc tggccgccgg ggacggcgcc gcgctgctgg gcttgatgga
     1021 gggcgcctcg caggcgcgcc gcaactggca tctgggcagc gggcggctga acaacgaaaa
     1081 tacgggatcg ccgaagtcgc catgaagctc gatcatctcg atctgccgcg cgcgaccggg
     1141 gcccggggca ccgtggcgct gccgggttcc aagagcattt ccaatcgcgt gctgctgctg
     1201 gccgcgctgg ccgcgggcgt cacggagatt ggcggcctgc tcgattccga cgacacccgc
     1261 gtcatgctgg ccgccttgcg cctgctgggc gtgccggtcg aggacctggg gccgggcagc
     1321 gtgcgggtgc ggggcaccgc gccctttccg gtcgaacagg ccgagatctt cctgggcaat
     1381 gccggcacgg ccttccggcc gctgacggcg gcgttggcgc tgatgggcgg cgactaccgc
     1441 ctgtcgggcg tgccgcgcat gcacgagcgc cccatcggcg acctggtcga ggcgctcaac
     1501 ggcctgggcg ccagggtggg ctacctgggc cagcctggct atccgccctt gcgcatcggc
     1561 cgcggccggc cgcgcgccga tgccccggtg cgggtgcagg gcagcgtgtc cagccagttt
     1621 ctcacggccc tgctgctggc cgcgcctctg ctggcgcgcg ccgcgggccg gcctgtcacc
     1681 ctcgaggtca tcggcgaact gatctccaag ccgtatatcg agatcacgct caacctgatg
     1741 gcgcgctacg gcgtggcggt ggcgcgcgaa ggctggcagc gcttcatcgt gccggccgac
     1801 gcggcctacc tcagcccggg gcgcatcctg gtcgagggcg acgcgtcgtc ggcctcgtac
     1861 ttcctggcgc tgggcgccat cggcggcggt ccggtgcgcg tgaccggcgt gggcgccgac
     1921 agcatccagg gggacgtggc ttttgcccgg accctgcagg ccatgggcgc gcgcgtcgaa
     1981 tccggccccg actggctgca ggccagcggc gtgaacgcgg cccggggcga gacgctgcgc
     2041 gccttcgacg ccgacttcaa tctgatcccc gacgccgcca tgacggccgc cgccctggcc
     2101 ctgtatgccg acggcccgtg cctcctgcgc aatatcggca gctggcgcgt caaggaaacc
     2161 gaccgcatcc acgccatgca gaccgaactg gccaagctgg gcgcgcaggt cgagtccggc
     2221 cccgactggc tgcgggtggc tccgccagcg caatggcgcg atgccgacat aggcacctat
     2281 gatgaccatc gcatggcgat gtgtttttcg ctggcggctt tcggcccggc caaggtgcgc
     2341 atcctggagc cggggtgcgt gagcaagacc ttcccgacgt atttcgatat ttatgcggac
     2401 ttgatccaga cctgagccga ggaccccgca gaaatccgaa gcacagcccc caccgggggc
     2461 gggcatcggc attccgcagc gcagcctcgg tttctcaaga actcaaaacg ccatgagcca
     2521 tatcgttcca gtcatcacca tcgacggccc gaccgcctcc ggcaagggca ccgtggccca
     2581 tcgggtcgcc caggccctgg gctgggcggt gctcgacagt ggcgccctgt accgcctgac
     2641 cgccaaggcg gcgatcaagc acggcatcgc ggccgaggat gccccggccc tggcccgggc
     2701 ggcccagtcg ctggacgcgt gtttcgaggg agaacgcatc cttctggaag gagaagacgt
     2761 cacggacgtc atccgccagg agcaggtggg caacctggct tcgcgcatcg ccggctaccc
     2821 cgaactgcgc caggcgctcc tggcccgcca gcgggctttc cgggtcgcgc cgggcctggt
     2881 ggccgacggc cgagatatgg ggacggttgt ctttcccgac gcgccgctca aggtatttct
     2941 catcgccgct gccgaggcga gggcgcaaag acgctataag caattgatcg ataagggaat
     3001 ttctgctaac ataccggacc ttttgcgaga tttgcaggct cgggacgcgc gcgatatgca
     3061 gcgcacggcc gcgccgcttg ctcccgcgga ggatgcacgg gagctggatt cgtcgaacct
     3121 gactatcgag cagacggtgc aggcagtatt gagtatgtgg caggctcgct gagcctgcct
     3181 tgccatgtcg tagcacggcc ctgtcggcgc gtcgcatgca atgcatgcgc gttggccagc
     3241 aggttttatc actccactgt gccagcgcgc atgttcgctg aacgcggtgt gtaatttaac
     3301 ggccaatcct ggcccatgga ccttcaacct aatgtcttcc cttaaccagc aagtctccgg
     3361 cggcgaaagc tttgccgacc tgtttgccca aagcctcaag agccaggaca tgaagtccgg
     3421 cgaggtcatc agcgccgaag tcgtacgcat cgaccacaac ttcgtggtcg tcaacgccgg
     3481 cctgaagtcc gaagcgctca tcccgctcga agaattcctc aacgatcatg gcgaagtcga
     3541 agtccaaccc ggcgactacg tctcggtggc catcgattcc ctggaaaacg gctacggcga
     3601 caccatcctg tcgcgtgacc gcgccaagcg cctgtcggcc tggctgtcgc tggaaaccgc
     3661 gctggaaaac ggcgaactgg tcaccggcac catcaccggc aaggtcaagg gcggcctgac
     3721 cgtcatgacc aacggcatcc gcgccttcct gcccggctcg ctggtcgacc tgcgtcccgt
     3781 caaggacacc acgccgtacg aaggcaagac cctcgaattc aaggtcatca agctcgatcg
     3841 caagcgcaac aacgtcgtgc tgtcgcgccg ccaggtgctg gaagccagca tgggcgaaga
     3901 gcgccagaag ctgctcgaaa ccctgcacga aggcgcagtg gtcaagggcg tggtcaagaa
     3961 catcaccgac tacggcgcgt tcgtggacct gggcggcatc gacggcctgc tgcacatcac
     4021 cgatatggcc tggcgccgcg tgcgccaccc ctccgaagtc ctcaccgtgg gccaggaagt
     4081 cgaagccaag gtgctcaagt tcgaccagga aaagagccgc gtctccctgg gcgtcaagca
     4141 gctgggcgaa gatccgtggg tgggcctggc tcgccgctac ccgcaaggca cccgcctgtt
     4201 cggcaaggtg accaacctga ccgactacgg cgcgttcgtt gaagtcgaag ccggcatcga
     4261 aggcctggtg cacgtgtccg aaatggactg gaccaacaag aacgtcgatc cgcgcaaggt
     4321 cgtgaccctg ggcgaagaag tcgaagtcat ggtcctggaa atcgacgaag accgtcgtcg
     4381 tatctcgctg ggcatgaagc agtgccgtca gaacccgtgg gaagagttcg ccacgaactt
     4441 caagcgcggc gacaaggtcc gcggcgccat caagtcgatc accgacttcg gcgtgttcgt
     4501 cggcctgccc ggcggcatcg atggcctggt gcacctgtcc gacctgtcct ggacggatac
     4561 cggcgaagaa gccgtgcgca acttcaagaa gggcgacgag ctcgaagccg tggtcctggg
     4621 catcgacacc gacaaggaac gcatctcgct gggcgtcaag cagctcgaag gcgatccctt
     4681 caacaacttc gtcgccacgc atgacaaggg cgccgtcgtt ccgggcacga tcaagtcggt
     4741 cgagcccaag ggcgtcgtcg tgaccctgtc ggtcgacgtt gaaggctacc tgcgcgcttc
     4801 cgagatctcc tcgggccgcg tcgaagacgc caccaccgtg atgaaggccg gcgacaacat
     4861 cgaagccatg atcatcaacg tcgaccgcaa gacgcgctcg atccaactgt cgatcaaggc
     4921 tcgcgacaac gccgaaaccg ccgagaccat ccaacgcatg tccgaagcca gcgcttcgtc
     4981 gggcaccacg aacctgggcg ctctgctcaa ggccaagctc gaccagcagc gcggcgaagg
     5041 ctgatagcgg gttgactcag tgaccaagtc ggagctgatc gcagcgttgg cggcccgcta
     5101 tccccagctg gccgcccgcg acaccgatta cgccgtcaag accatgctcg acgcgatgac
     5161 ccaggccctg gcctcgggcc agcgcatcga gatccgcggt tttggcagtt tctcgctgtc
     5221 gcaacggtct ccccgggttg ggcgcaatcc caagtcgggc gagcaggtgc tggtgcctgg
     5281 caagcaggtg ccgcacttca aggccggcaa ggaactgcgc gagcgtgtcg acctggtcgc
     5341 cggcgaactc gccggcggcg ctgacgggtc ttccgacccc acgtcatccg aactcgacct
     5401 gcacgccatg cattgagcgt tgcagcgccg gataaaaaag gcctttccag gcgactggaa
     5461 aggccttttg ctttgggccc ctgcatgcag ccgtcttcgc ctcccagggc ccgcgccttg
     5521 cgcaggggct tgggttttgc ctgcggcgag atgatgttaa ggtttagtcg atgcgacatc
     5581 ttgaagtgat cgattacggc gtcatcgccg tgtattttgc gttgacggca ttcatcgggt
     5641 tttccgccac gcggcgcgcc acctcctcgg atgatctgtt cctggccgga cgcagcctgg
     5701 gaccggtggt cgtgggcttt tccctgttcg cctccaacat ttcgtccgac acgctggtgg
     5761 ggctgcccgg cgcggcctac cgcaacggca tctcggcatc caactacgag tggatggccg
     5821 gcgtggtgct ggtcttcacg gcgatctttg tgctgccggt gctgatgcgc tcgcgggtgg
     5881 cgaccatgcc cgagttcatg gaacggcgtt tcgactggcg cctgcgccgg tatttgtcgg
     5941 tcatcacgct ttttctctcc atcatcctgg atacggcggg caccttgtac gcgggcagcc
     6001 tggtgctgtc gatcttcatc cccgggctgg cgttgtggca ggcgtgctgg gtcctggcgc
     6061 tgttcaccgc gctttatacg gcctgcggcg gcctgcgtgc cgtggtctat gccgatatcc
     6121 tgcaggcgct ggtgctgctg ggcggttcgg cttgcctggc catcatcatg tttggccact
     6181 tcgactacag ctggtcggtg ctgacggcac gggtggatgc gaacaagctg tcgctgatcc
     6241 gtccgctgga cgatccgggc gtgccctggc tggggctcgt cacgggcctg cccatcgtgg
     6301 gcttttatta ctggacgatg aaccagtatg tggtgcagcg gatattgggc gcgcgcaacg
     6361 cggccacggc gggccgcgcg gcgctctttg ccgcgctgct caagctcctg cccattttcc
     6421 tgatgaccat cccgggcgcc atggccagcg tattgctgcc ggggctggcg cagcccgacc
     6481 aggtgtatcc cgccatggtg tcggcctttg cgccgaccgg catccgaggc ctgatcctgg
     6541 cgggcctgat ggcggcgttg atgtccacgg tgtcggccac cttgaattcg gccgccaccc
     6601 tggtggcgct ggatttcgtg cagccggcgc ggccgaggct gacgtcctcg caattggcct
     6661 gggtggggcg ggtggccacg ctgttcatcg ccctgattgc cgcggcctgg gcgccgatga
     6721 tccagcactt tgccggcctg tggggctatt tgcagcaggt gttcgccttt gtggcctcgc
     6781 cgctggtggc gaccttcctg atggggctgt gggtggcgcg cctgggctcg tcggcggccc
     6841 tgcgcggcct ggtgtgcggg catgtgctgt ccgcggtgtg cttcgtgctg taccaggccg
     6901 gtatcgtgca cctgcatttc accgtgctgg gcggcgtgct ctgcgccgcg acggcgctta
     6961 tgacctgggc ctggatgcgg gtgctgcggg atcgcgacca gcctgacggg caggggcggg
     7021 aaatcctgtc gcatgcccgg ttcaacctgg ccggcatgcc ccgcgacgtg ctgtggggct
     7081 gcgccgcggt gctggtgctg accggcgtga tcgtggtggc cctgcgctga aagacgccgg
     7141 tgcggacgcc tgccggcgtc cgcctccggt gcgatcccta cgccgcttcc aatgccgccg
     7201 cgaccgccat gccggcctcg gtggtatcga gcttgccgcc caggtcgccc gtggtctggc
     7261 cggcggcgag cgtctgctcg atggcgcgca tgatggcgtc gtgggcctgg cggtagacgc
     7321 cctggccgct gcccaggaag tccagcatca tggcgcccga ccagatcatg ccgaccgggt
     7381 tggcgatgtt cttgccgtag atgtcgggcg ccgagccgtg cactggctcg aacagcgagg
     7441 gcagggtgcg gtcggggttg atgttggccg agggagcgat gccgatggtg ccggtgcagg
     7501 ccgggcccag gtcggacagg atgtcgccga acaggttcga agccaccacc acgtcgaagc
     7561 gctcggggct gatgacgaaa cgcgccgtca ggatgtcgat gtggtacttg tcctggcgca
     7621 cgtcggggta ttgcttgccc atctcggcga agcgttcgtc ccagtagggc atgctgatga
     7681 agatgccgtt ggacttggtg gccgaggtca ggtgcttctt ggggcggctt tgcgccagct
     7741 cgaaggcgta cttgatgatg cggtcggtgc ccttgcgcga gaagatcgat tcctggatga
     7801 cgaactcgcg gtcggtgccg ggaaacatct tgccgcccag ggacgaatac tcgccctcgg
     7861 tgttctcgcg cacgatgtac atgtcgatgt cgcccggctt gcggccggcc agggggcagg
     7921 gcacgcccgg catcaggcgg gcagggcgca ggttcacgta ttcgtcgaaa tcgcggcgga
     7981 acttgatcag cgacccccac agcgaaatat ggtcgggcac gatgtccggc cagcccaccg
     8041 cgccgaaata gatggcctcg cagtccttga gctggtcgaa ccagtcgggc ggcatcatgt
     8101 cgccgtgctt ggcgtagtag tcgcaactgg cccactcgat gtgcttgaac gccagcttga
     8161 tgccgaagcg ttttgccgcg gcctcgacca cgcgcaggcc ttcgggcatg acttccttgc
     8221 cgattccgtc gccggcaatg acggcgatct tgtgcgtttt cacttccctc tccccttgct
     8281 tggtttgtcc atggattgga gggccaatct agcacggctg cagccgggtg ggccaagggg
     8341 gccggcgcaa gttacaatcc gaggtccgaa ttcctgctgg gagcctgtgt catgcgctac
     8401 ctcatttggg tcctgcgact gcttgtcttc attgcggtgc tgatcttcgc cgtgaagaac
     8461 accgaccccg tcaaggtcaa tttctacgcc gactacacgg tgcagagcgt gccgctcatc
     8521 gtggtcatgc tggtgacctt cgtggtgggc accgtcttcg gcctgctgtt gacggtgccg
     8581 gtggccatgc ggcggcgccg cgaggcctcg cgcctgcgcc gcgaagtcga gcgcctgcag
     8641 gccgcggccc accccgccga cgtgacgccg gccatggtgc cgccggagtc catcgccccc
     8701 atgtcgccgc tctgagccgg ctgtttttgt tttcgagcct gattcgtgga ttttgaacct
     8761 tggtggttga ttttcattcc catgctgttt gccttgggat ggctggccgc gcgcttcgat
     8821 ttccggcaga tgctgcgtga aacgcgcacc ctgccggact cgtatttccg cggcctgaat
     8881 ttcctgctca acgaagagcc cgaccgggcc atcgacgcct tcgtcgaggt ggccaagctc
     8941 gacccggaaa ccaccgagct gcactttgcc ctgggcagcc tgtttcgccg ccgcggcgag
     9001 atggagcgcg ccatccgcgt gcaccagagc ctgctcaacc gcgccgacct gccggtggcc
     9061 gagcgcgacc acgcccagca cgagctggcg caggacttcc tcaagtccgg catgctcgac
     9121 cgcgccgaaa gcaccttcag cacgctcaag gacacgcgct acgccttgcc ggcgctgcgt
     9181 tcgctggtgc gcatctacga gtccgagcac gactggccgc gcgccatcga ggtggtcaag
     9241 accctgcagg gcctggtcga cgagcccgtg ccgcagatcg tgcattacca ctgcgaactg
     9301 gcgctgacgg cgctctcggc caagccggtg aacttcgagg ccgccaacgt ggcgctggac
     9361 gccgccgacc atgcggccaa gagcttcgac gccgccatct cgcgcggcgc ccgggtgcgc
     9421 atcgccatgc tgcgcgcgca gatggcgcag ctcgagcaga accccaagcg cgagcgcctg
     9481 tacctggagt ccatcctggc gagcgcgccc gagtacgccg gcctggtggc cgagcgcctg
     9541 ctggccaatt acacccagac gggcgaggcg gccgacggcc tggacgtgct cagccggcat
     9601 tatgcgcagc atgcctcgct ggatctcttc aatgtggtgt tccgcgagct gcgggcccag
     9661 cgcggcgcgg cgccggcctg gcgctttgcc cgcgaggcgc tgcgcagcca tccctcgctg
     9721 ctgggactgg accgcctgct cgaggccgaa ctggccaacc cggtgccggc cgccgcgatc
     9781 gcgggcgccg atgagtcggt gcccagcgat acgcgccgtg gcgaggccgt ctccggcggc
     9841 gacctgaccc tgctgcgcag tctcatccac aagcacacgc agcggctcga ccgctatgtc
     9901 tgccggtctt gcggtttcca gccgcgcagc ttctattggc agtgcccggg ctgcaattcc
     9961 tgggagacct cgtcgccccg ccgcctggag gaactggaat gatggccgat ccgtcctttc
    10021 ccgcccaggc catctcgcgc agccgcgtgc tggtggtggg cgacgtcatg ctcgaccgct
    10081 actggttcgg cgaggtcgac cgcatttcgc ccgaggcgcc ggtgcccgtg gtgcacgtgg
    10141 cccgccgcga agaccgcctg ggcggcgccg ccaacgtggc gcgcaatgtg gcggccctgg
    10201 gcggactggt cacgctggtg ggcgtgacgg gctccgacga ggccggcgcg cgcatcggcc
    10261 agctcgcgca agaggccggc gtgcagtccg acctggtcag cgatgccgag caccccacca
    10321 cgctcaagat gcgcgtgctg ggccgccagc agcaattgct gcgcatcgat ttcgagcagc
    10381 atccgtcgga tggcgcgctc gacggagtgg gcgcggcggt ggcgcgccat ctgccgcacc
    10441 acgacgtggt ggtgctgtcg gactacgcca agggcgcgct cgagcgcgtc gagtccctga
    10501 tcgcgctggc gcgccagcac aaggtgccgg tgctggtcga ccccaaaggc gacgattaca
    10561 cgcgctacaa aggggccacg ctggtcacgc ccaaccgttc cgaaatgcag ctggccgtgg
    10621 gccgctggaa gagcgaggac gacctgaccg cgcgcgccca gcaattgcgc gccgatctgc
    10681 agctcgaagc cttgctggtg acgcgctcgg agcagggcat gaccctgttc gccgacgcgg
    10741 ggcgcgagca cgtcgatgcc caggcgcacg aggtgttcga cgtgtccggc gcgggcgaca
    10801 ccgtgctggc caccctggcc ctgatgcgcg ccgccggcct gtcctgggcc gaggccatgg
    10861 gctgggccaa ccgcgccggc ggcatcgtgg tgggcaagct gggcacctcc accgttaccg
    10921 ccgcggaact ggcaggagtc tgatcatgtt tatcgttact ggcgccgccg gcttcatcgg
    10981 cagcaacctg gtgcggggcc tgaaccgccg cggcatcgac gatatcatcg cggtcgacga
    11041 cctcaccgac ggcgacaaat tccgcaatct ggccgactgc cgcattgcag actatctcga
    11101 caaggacgat ttccgcgccc gcgtgctgga aggccggctg cccgacgtgc gcgccgtgct
    11161 gcatcaggga gcctgctcgg acaccaccga gcgcaacggc cgcttcatgc tcgacaacaa
    11221 ctaccgcgtc acgctggagt tgttcgagta ctgccagagc cgcttcattc ccttcctgta
    11281 tgcctcgtcg gccgcggtgt acggcggttc gtcggtctat gtggaagacc cggccaatga
    11341 ggcgccgctg aacgtctacg gctattccaa gctgttgttc gaccaggtgt tgcgccgccg
    11401 catggcgcag cgcacctcgc aggtggtggg gctgcgctac ttcaacgtgt acggcccgca
    11461 cgagcagcac aagggccgca tggcctcggt ggctttccac aacatgaacc agttcctggc
    11521 cgaagggcat gtgcggctgt tcggcggctg ggacggctac ggcgacggcg agcagcgccg
    11581 cgacttcatt tcggtggagg acgtggtggc cgtgaacctg tacttcctcg accaccctga
    11641 acagtcgggc attttcaact gcggcaccgg ccgcgcccag cccttcaacg acgtggccgc
    11701 cgccgtggtc aacgccttgc gcgccgagcg cggcgaggcg gccctgccgc tggccgagct
    11761 ggccagggcc gggttcgtcc gctacatccc tttccccgac gacctcaagg gccgctacca
    11821 gagctacacc caggccgacg tctcgaacct gcgcgccgcg ggtttcgccg cgcccatgcg
    11881 cgacgtgcag accggcgtgg ccgaatacgt gcgcgcctgg cgcgcccaat aaggccgcat
    11941 ttcccgaggc cgtacttccc gcaacggaca tttccgttca ccgcgtctac ccgcatcatg
    12001 cgccgctgcg catgatggct tgccgggccg tcatccgacc gcccggcaca ccaggagatg
    12061 cgcatgaatc cctttctgca cgaccccgtt gcccggcccg cgccgcgcgc ggatgtcccg
    12121 accgccgttg ccgcgtccgc caggcgcatg cgccgccgct tgatggccgg cccgatgggg
    12181 cgcctggccg cggcgggcgg cctgagcctg gccatggcct cggcccatgc gctcgacgtc
    12241 aacgctgcca gcgccgaaca actcgaggcc ttgcgcggcg tggggcccaa gacggcgcgg
    12301 atcatcgtcc aggaacgcct gcgcggcggg cgcttcgaat ccatcgagga cttgtccgac
    12361 cgggtgcggg gcatcggccc gaaacgggcg caggccttgc aggcgtccgg gttgaccgtg
    12421 ggcgccggcg ccgcgtctgc ccaggccgcg ggcgcggcgc ccaggccggc ggcaccgaaa
    12481 tcgccgggca ggccctgaag gcggtcgccg gggcggccat catcttgggg tggccacctt
    12541 caggcggcca ccctacgtca aatatggcta tgcggcgatg ggggtgggcc tggaggtatg
    12601 atcatcgctc gaaatcctag gcatttggca tgaatatcac cgattacccc accatcgaac
    12661 agaccatcgg caatacgccg ctggtgcgcc tgcagcgcat ccccggccag gcaggcgcgg
    12721 cgcgcggcaa cgtcatcctc gccaagctcg aaggcaacaa ccccgccggt tccgtgaagg
    12781 acaggccggc catgtccatg atccggcgcg ccgaagagcg cggcgagatc aagcccggcg
    12841 acaccctcat cgaggccacc agcggcaaca ccggcatcgg cctggccatg gccgcggcca
    12901 tgcgcggcta ccgcatgatc ctcatcatgc ccgacaacct ttcgctcgag cgccgcgcct
    12961 ccatgacggc ctacggcgcg cagctcatcc tcacccccgc cgacaaaggc ggcatggaat
    13021 acgcgcgcga cctcgccacc gaaatgcagt ccgaaggcaa aggcaaggtg ctcgaccagt
    13081 tcgccaatcc cgacaacccg cgcgcccaca tcgagaccac cggccccgag atctggcagc
    13141 agacgggcgg ccgcattacc cacttcgtca gcgccatggg gaccaccggc accatcatgg
    13201 gcgtgtccac ctacctgaag ggccgcagcc ccgccatcca ggtcatcggc gcccagccgg
    13261 ccgaaggctc gcagatcccc ggcatccgca aatggcccga ggcctacctg cccaagatct
    13321 tcgacgccgg ccgcgtcgac gcctacgagt ccatcgagca gaaagacgcc gaagtcatgg
    13381 cccgccgcat ggcggccgag gaagggatct tcgccggcat ctcgtcggcc ggcgccctga
    13441 tcgcagccct gcgcgtggcc gagcgcgcca gcgacgccac catcgtcttc gtcgtgtgcg
    13501 accgcggcga ccgctatctg tcgacgggcg tgttcaatcc gtaatttgca attcgcgggt
    13561 gctgcttgcg atcgtccagg cagtagataa tgcagtctgc cgagggcggc gggtgtggcg
    13621 gggcgcctca gcggcggggc agcccgcgca gcgggccgag ggcgcctgat ggcgcgcccg
    13681 agcccgacgc tgcgaagccc cgccataccc gccgccctcg acagacgttg caagaaccaa
    13741 agccctacaa cactctctac aacgcccccg ccgaagcagg cgccacctgc gcagccaccg
    13801 catccgccag atccgacacc gccgtcgcgt agaaataact gtggttgtac tgcgtgatgg
    13861 caaagaaatt cggcgtgccc gtgcggtatt gcgccgtgcc gcgcgcttcc tccaccaggt
    13921 ccacgatgcc cagcggctgc ctgctccagg cgccttcgcc gccgccggcc gacacccgcg
    13981 cgcccgcggc cttgagcttg tcccagtcca gcgtcggctc gagcccgccc gccaccaaag
    14041 ccgaaggatc cgcgggcagc gtcaccggtg caaacaccgg cacgccgcgc acccagccgt
    14101 gcgaagccag gtaattgccc accgacatga tggcgtccgc cggattgttc gccagatcga
    14161 tgggcccgcc ggtattgccc gaaccgtcca ccgcatagcg cgtaatgctg cccggcatga
    14221 actgcggcat gccgatcgcg ccggcgtacg agccgcgcgt gtccatgtcc aggcggccct
    14281 gcagcgccag cgtgatgaaa tccgccaact ggccgcggaa catctcggcg cggtccggct
    14341 tgtcgggcac ggggtaatcg aaggccagcg tcgcgagcgc gtccatcacc cggaaactgc
    14401 ccatattgcg gccatagatc gtttcgaccc cgatgatgcc ggcgatgatc ttcgccggca
    14461 cgccataacg ctgcgccgcc tggttcagcg tggcggcatt ggcctgccag aactcggcgc
    14521 cccagcggat gcgcttgggc tcgacgaagc gcgagcggta ggtgagccag ctgcgccaca
    14581 ccttgtggtc gggcgtggac ggcaggatca ggcgcgccac cgtggcgctg tagcgcgcgc
    14641 tggacaggct gcccagcacc cggtccagcg gcaggttctg gcgggccgcc aggtcgcgcg
    14701 cgaaggcatc cacctcgggg cgcaggcggc cgctgggcag gacggtggcc gagggttcgc
    14761 cggaggattc cggcgcggtg ggctcggacg ggccgatacg gatcgggctg gaagacgatg
    14821 cggtggaggg aagatcgggg gcggtggctg gcgggtgggg cgatgcgcag gctgagagaa
    14881 gaaaaggggt cagcacgccg atttgcagta aacgcctaca gatgaacata atcttcccta
    14941 tggagacgct ctatcttacc cacccgtcgt gccggctgca cgaaatgggc aactggcacc
    15001 ccgagagccc ggagag
//
LOCUS       DASWPO010000369         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bordetella sp. isolate SMAG_U3469 k141_714205, whole
            genome shotgun sequence.
ACCESSION   DASWPO010000369 DASWPO010000000
VERSION     DASWPO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959287
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bordetella sp. (soil metagenome)
  ORGANISM  Bordetella sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Alcaligenaceae; Bordetella.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 13:43:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,955
            CDSs (total)                      :: 3,918
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 37
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 7 of 37
            Pseudo Genes (incomplete)         :: 18 of 37
            Pseudo Genes (internal stop)      :: 13 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Bordetella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714205"
                     /isolate="SMAG_U3469"
                     /isolation_source="soil"
                     /db_xref="taxon:28081"
                     /environmental_sample
                     /country="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..969)
                     /gene="ugpA"
                     /locus_tag="VGC69_19750"
     CDS             complement(88..969)
                     /gene="ugpA"
                     /locus_tag="VGC69_19750"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEY0297571.1"
                     /translation="MEKRVVFGHKTLPYLLLAPQLLITVIFFFLPAGQAIWQSMRLED
                     AFGLSSQFVGLENFADLFSQQAYLDSFKVTAVFSILVAFVGLALALLLAVMANRVVRG
                     ASVYKTLLIWPYAVAPAVVGVLWLFLFSPSVGILAVALKILGVNWNPRLDGPQAMTLV
                     LIAAVWKQISYNFLFFLAGLQSIPRSLIEAAAIDGASPVRRFWTIVFPLLSPTTFFLL
                     VINVIYAFFDTFAVIDTTTQGGPGTATDILVYKVYSDGFRGLDLGSSASQSVVLMFIV
                     VALTVVQFRYVDRKVQY"
     gene            complement(1133..2443)
                     /gene="ugpB"
                     /locus_tag="VGC69_19755"
     CDS             complement(1133..2443)
                     /gene="ugpB"
                     /locus_tag="VGC69_19755"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEY0297572.1"
                     /translation="MRFSHLALAVAATGVFGITSAQAATEIQFWHSMEGALGDRVNAI
                     ADDFNKANPDYVIKPVYKGSYGESMNAGIAAFRAGNAPDILQVFEVGTATMMYAKGAI
                     KPVQQMSEEAGDPIDPKAFIGAVAGYYSSADGKLVSMPFNSSTVVFYYNKDAFKKAGL
                     DPEKPPKTWEELAADGAKLKAAGMECGYTTGWPSWSQLETFSAWHNVPYATKDNGFGG
                     LDARLAINTPLHQRHLENLAKLAKDGIFMYGGRGDDPNALFISGKCAMITGSSGMRAN
                     VIKNAKFDFGTSTIPYYSDVKGAPQNAIIGGASLWVFANKKPEVYKGITKFFKYLASP
                     SVAASWHQNTGYVPVTKAAYELTKSQGFYDKNPGTDVGVQQLNVQTTAQSRGVRLGFL
                     PQIREIEDADIESIVSGKMSAKDGLADMTKRGDELLEKFQKSVN"
     gene            2655..3485
                     /gene="proC"
                     /locus_tag="VGC69_19760"
     CDS             2655..3485
                     /gene="proC"
                     /locus_tag="VGC69_19760"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY0297573.1"
                     /translation="MENITLAFIGGGNMATALASGLAGKVCAAGDIHVIDVNEAGHAA
                     WRARGMTVASAPGEMLRRSKVWVYAVKPQQMREAVRQSQPWLAPDTLVISIAAGLRAD
                     TLAGWLGGPGQPWRRLVRCMPNTPALVGAGITGLAATPGVTEADRELAGRLLQSVGEV
                     VWVDGDAGLDAVTALSGSGPAYVFLLLEALAAGGQALGLTEAQARQLAQATVAGSAKL
                     AGQSSDTPAVLREKVTSKGGTTAAALDVFRQAGFADTVRAAMDAAARRSRELAEEFGK
                     "
     gene            complement(3488..3952)
                     /locus_tag="VGC69_19765"
     CDS             complement(3488..3952)
                     /locus_tag="VGC69_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0297574.1"
                     /translation="MSKEKTSFVYVTYIRSTPEKVFEAITQPDIARRYWGHENVSDWQ
                     PGSTWEHVRANEQRTVELLGKVVEAAPPTRLVITWANASQAADPASYSRVSFDIAEYE
                     EMVRLTVTHDELEAGSGMAKGIRQGWPIVLSSLKSLLETGQGIDVFAKPKAA"
     gene            complement(3949..4269)
                     /locus_tag="VGC69_19770"
     CDS             complement(3949..4269)
                     /locus_tag="VGC69_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0297575.1"
                     /translation="MDADKVFKALADPTRRKLLDLLYESNGQTLGQLCEHLDMARQSA
                     TQHIGVLEAANLVSTVRRGREKLHFINPVPLHEVYERWVRKFERQRLGLLHDLKKELE
                     GEQP"
     gene            complement(4380..>4520)
                     /locus_tag="VGC69_19775"
     CDS             complement(4380..>4520)
                     /locus_tag="VGC69_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY0297576.1"
                     /translation="NSQHRADAMKSWLHHYNWHRPHQGIGRAVPISRLNLDGYNLLTL
                     HS"
BASE COUNT          748 a         1320 c         1618 g          834 t
ORIGIN      
        1 catcgccacc ccgcagagca gaatgacgtg ggccagaata tccagccagg gacggcgttc
       61 aaccattgtt attagcctcg gacaaaatca atactgcact ttgcggtcga cgtaccggaa
      121 ctgtacgacc gtcaaggcaa ccacaatgaa catcagcacg accgactggg acgcggagga
      181 acccaggtcc aggccgcgga agccgtcgct gtacaccttg tagaccagga tgtcggtggc
      241 cgtgccgggc ccgccctgcg tggtggtgtc gatcaccgcg aaggtgtcga agaaggcgta
      301 gatgacgttg atcaccagca ggaagaaggt ggtgggcgag agcaggggga agacgatggt
      361 ccagaagcgc cgcacgggcg aggcgccgtc gatggcggcg gcctcgatca gcgagcgggg
      421 gatggactgc aggccggcca ggaagaacag gaagttgtag gagatctgct tccagacggc
      481 ggcgatgagc accagggtca tggcctgcgg gccgtccagg cgcgggttcc agttcacgcc
      541 gaggatcttg agcgccacgg cgagtatccc gaccgagggc gagaacagga acagccacaa
      601 tacgcccacc acggccggcg ccacggcata aggccagatg aggagcgttt tgtagacgct
      661 ggcgccgcgc accacccggt tggccatcac ggccaggagc agcgccaggg ccaggcccac
      721 gaaggccacc aggatggaaa atacggcggt gaccttgaag gaatccaggt aggcctgctg
      781 cgagaacagg tcggcgaagt tttccagccc gacgaattgc gacgaaaggc cgaaggcgtc
      841 ttcgagccgc atggattgcc agatggcctg gccggcgggc aggaagaaga agatgaccgt
      901 gatcagcagc tggggcgcca gcagcagata gggcaatgtc ttgtggccga aaacgacgcg
      961 tttttccatg ggcagggcgg gttccggagg cgctggagcc tgatggccgc aagcataacc
     1021 gcaaaattga aacagctttt gaaagacaag ggggcgaact tttcccgggg ggaaaagcgc
     1081 ccgccttgcc gaaggcgcat gcgcgcgggc gggcggtttg cccgccgcgg gatcagttca
     1141 cgctcttctg gaacttctcg agcagttcgt cgccgcgctt ggtcatgtcg gccaggccgt
     1201 ccttggcgga catcttgccg gacacgatgc tttcgatgtc ggcgtcctcg atttcgcgga
     1261 tctgcggcag gaagcccagg cgcacgccgc gcgactgcgc ggtggtctgc acgttgagct
     1321 gctgcacgcc gacgtcggtg ccggggttct tgtcgtagaa gccctggctc ttggtcagct
     1381 cgtaggcggc cttggtgacg ggcacgtagc cggtgttctg gtgccaggac gccgccacgg
     1441 aagggctggc caggtacttg aagaacttgg tgatgccctt gtagacctcg ggcttcttgt
     1501 tggcgaagac ccacagcgag gcgccgccga tgatggcgtt ctgcggcgcg cccttgacgt
     1561 cgctgtagta ggggatggtc gaggtgccaa agtcgaactt ggcgttcttg atgacgttgg
     1621 cgcgcatgcc ggacgagccg gtgatcatgg cgcacttgcc gctgatgaag agcgcgttgg
     1681 gatcgtcgcc gcgcccgccg tacatgaaga tgccgtcctt ggcgagcttg gccaggtttt
     1741 ccaggtggcg ctggtgcagc ggggtattga tggccaggcg ggcgtcgagg ccgccgaagc
     1801 cgttgtcctt ggtggcgtag ggcacgttat gccaggccga gaaggtctcg agctgcgacc
     1861 acgacggcca gccggtggta tagccgcact ccatgccggc ggccttgagc ttggcgccgt
     1921 cggcggccag ttcttcccag gtcttgggcg gcttttcggg gtccaggccg gccttcttga
     1981 aggcgtcctt gttgtagtag aagaccaccg tcgagctgtt gaagggcatg gacaccagct
     2041 tgccgtcggc cgacgagtag tagccggcca ctgcgccgat gaaggccttg ggatcgatcg
     2101 ggtcgccggc ttcttcggac atttgctgca ccggcttgat ggcgcccttg gcgtacatca
     2161 tggtggcggt gccgacttcg aacacttgca ggatgtcggg ggcgttgccg gcgcggaagg
     2221 cggcgatgcc ggcgttcatg gactcgccgt agctgccctt gtagacgggc ttgatgacgt
     2281 agtcggggtt ggctttgttg aagtcgtcgg caatggcgtt cacgcggtcg cccagggcgc
     2341 cttccatcga gtgccagaac tgaatttcgg tagcggcttg ggccgaggtg atgccgaata
     2401 cgccggtggc ggcgacggcg agggccaggt gtgagaatcg cattgcaact tcctccaggg
     2461 gactatcggc acggcgccgg tatggtgtgc gcgggctgaa tgaccgcacc gggtacccac
     2521 tgggcctcgg tgcgaacccg gcactttatg aattgtttat gacacgggcg tgactttcac
     2581 attcagcatg gtggctgtca atggtccggg tctccgtctc ctcgtttctt gatgcctcgg
     2641 attacaatct tttcatggaa aacatcacgc ttgcgttcat cggcggcggc aatatggcca
     2701 cggccctggc ttcggggctg gcgggcaagg tttgcgcggc cggcgacatt cacgtcatcg
     2761 acgtcaacga ggccggccac gcggcctggc gggcgcgcgg catgacggtg gcgtcggcgc
     2821 cgggtgagat gttgcgccgc agcaaggtgt gggtctacgc ggtcaagccg cagcagatgc
     2881 gcgaggcggt gcggcagagc caaccctggc tggcgcccga tacgctggtc atcagcattg
     2941 cggccggcct gcgggccgat acgctggcgg gctggctggg cgggccgggc cagccctggc
     3001 ggcggctggt gcgctgcatg ccgaacacgc cggcgctggt gggcgcgggc atcacgggcc
     3061 tggcggcaac gccgggcgtg accgaggccg accgcgagct ggccgggcgc ctgctgcagt
     3121 cggtgggcga ggtggtctgg gtggacggcg acgccggcct ggacgcggtg acggccttgt
     3181 cgggcagcgg cccggcctat gtgttcttgc tgctggaagc cctggcggcc ggcggccagg
     3241 cgctaggcct gaccgaggcg caggcccgcc agttggcgca ggccacggtg gccggttcgg
     3301 ccaagctggc cgggcagtcc agcgatacgc cggcggtgct gcgcgagaaa gtgacctcca
     3361 agggcggcac gacggccgcg gcgctggacg tgttcaggca ggccggcttc gcggatacgg
     3421 tgcgggcggc gatggacgcg gcggcccgcc ggtcccggga actggccgag gaattcggca
     3481 agtaaattca ggccgccttc ggcttggcga aaacgtcgat gccttgccct gtttcgagca
     3541 gggacttcag gctggaaaga acgatcggcc agccctgccg gatgcccttg gccatgccgc
     3601 tgccggcctc gagttcgtcg tgggtgacgg tcagccgcac catctcctcg tattccgcga
     3661 tgtcgaagct cacccggctg tagctggccg gatcggcggc ctgcgaggcg ttcgcccagg
     3721 tgatgaccag gcgggtcggc ggcgcggcct cgaccacctt gccgagcagc tcgaccgtgc
     3781 gctgctcgtt ggcgcgcacg tgctcccagg tcgacccggg ttgccagtcg gagacgtttt
     3841 cgtggcccca gtagcgccgc gcgatgtccg gctgggtgat ggcctcgaac accttttccg
     3901 gcgtcgagcg gatgtaggtc acgtagacga agctggtctt ctctttgctc atggttgctc
     3961 tccttcgagt tctttcttca ggtcgtgcag caggccgagc cgctggcgtt cgaatttccg
     4021 cacccagcgc tcgtagacct cgtgcagcgg caccggattg atgaaatgca gcttctcccg
     4081 gccacgccgg accgtgctca ccaggttggc cgcctccagg accccgatgt gctgcgtagc
     4141 cgattgccgc gccatgtcca ggtgttcgca gagctgtccc agcgtctgcc cgttgctctc
     4201 gtaaagcagg tcgagcagct ttctgcgtgt cgggtcggcc agtgccttga aaaccttgtc
     4261 agcgtccatc cgttgccttc gtgcgtgagg agataatatg caggtaaata cctgcatgtc
     4321 aagcattgcc ttgatccacg cgccgcgctg tgccggcgcc gcaaggtgtg ctggcaggcc
     4381 tagctgtgaa gagtcaagag gttatatccg tcgaggttga gtctggagat ggggacagcg
     4441 cggccgatgc cctggtgtgg tcgatgccag ttgtagtggt gtagccagga tttcatggca
     4501 tcggctcgat gttgggagtt
//
